To what do firms attribute success? An application of attribution theory to the secondary woodworking industry

Science.gov (United States)

Matthew Bumgardner; Urs. Buehlmann

2015-01-01

Small firms are a critical component of the secondary woodworking industry and are important to hardwood lumber demand in the US. Understanding managers' perceptions of competitiveness in these firms is important to those working with the industry to help maintain a viable wood manufacturing base. One area of interest relative to the overall business environment...

ADVANCED DESIGN SOLUTIONS FOR HIGH-PRECISION WOODWORKING MACHINES

Directory of Open Access Journals (Sweden)

Giuseppe Lucisano

2016-03-01

Full Text Available With the aim at performing the highest precision during woodworking, a mix of alternative approaches, fruitfully integrated in a common design strategy, is essential. This paper represents an overview of technical solutions, recently developed by authors, in design of machine tools and their final effects on manufacturing. The most advanced solutions in machine design are reported side by side with common practices or little everyday expedients. These design actions are directly or indirectly related to the rational use of materials, sometimes very uncommon, as in the case of magnetorheological fluids chosen to implement an active control in speed and force on the electro-spindle, and permitting to improve the quality of wood machining. Other actions are less unusual, as in the case of the adoption of innovative anti-vibration supports for basement. Tradition or innovation, all these technical solutions contribute to the final result: the highest precision in wood machining.

Attraction of Foreign Investments in the Sphere of Economic Development of the Forest Resource Potential and Woodworking Industry: Institutional and Territorial Preconditions

Directory of Open Access Journals (Sweden)

Dzyubenko Oleksandr M.

2017-12-01

Full Text Available It has been found that it is possible to overcome the existing investment deficit in the forestry and woodworking segments of the forestry sector of the national economy on the basis of expanding the list of forms, methods and sources of attracting foreign investments into the sphere of economic development of the forest resource potential and wood processing. There revealed main trends in the dynamics of expenses for the maintenance and operation of equipment, as well as the maintenance and operation of forest roads across the regional forestry and hunting management departments. The priorities of improving the institutional environment for attracting foreign investments in the sphere of economic development of the forest resource potential and woodworking industry are substantiated based on implementing by state forest enterprises joint projects with foreign partners, as well as projects within the framework of public-private partnership agreements.

48 CFR 225.401-70 - End products subject to trade agreements.

Science.gov (United States)

2010-10-01

... Pub. L. 103-139. FSG Category/Description 22 Railway equipment 23 Motor vehicles, trailers, and cycles... Engine accessories 30 Mechanical power transmission equipment 32 Woodworking machinery and equipment 34... record equipment and ADP equipment 75 Office supplies and devices 76 Books, maps, and other publications...

Dating furniture and coopered vessels without waney edge - Reconstructing historical wood-working in Austria with the help of dendrochronology.

Science.gov (United States)

Klein, Andrea; Nemestothy, Sebastian; Kadnar, Julia; Grabner, Michael

In the present study, 208 furniture and 168 coopered vessels from three Austrian museums were examined. Dendrochronology was used to date objects and to extract further information such as the necessary time for seasoning, wood loss through wood-working and methods of construction. In most cases sampling was done by sanding the cross section and making digital photographs using a picture frame and measuring digitally. The dendrochronological dates of the sampled furniture range between 1524 and 1937. The group of furniture includes cupboards, chests, tables, benches, commodes and beds. In many cases furniture was artfully painted and sometimes even shows a painted year. With the help of dendrochronology it was proved that some objects had been painted for some time after construction, or had been over-painted. Most furniture, however, was painted immediately after completion. In this case, the seasoning and storage time of the boards and the wood loss due to shaping can be verified. As an average value, 14 years have passed between the dendrochronological date of the outermost ring and the painting. The time span includes time of seasoning and storage and the rings lost by wood-working. This leads, on the one hand to a short storage time of less than 10 years and on the other hand to very little wood loss due to manufacturing. Those boards being less shaped turned out to be back panels of cupboards, therefore they are recommended to be sampled for dating. Coopered vessels were dated between 1612 and 1940. There was evidence that staves were split and not sawn in many cases. The staves were often split out of the outermost part of the tree and hardly any wood was worked away which was proved by the close dendrochronological dates of the single staves of a vessel. Since there is a short time of storage and only little wood loss through wood-working, dating of objects without a waney edge becomes reasonable.

Improving machinery reliability

CERN Document Server

Bloch, Heinz P

1998-01-01

This totally revised, updated and expanded edition provides proven techniques and procedures that extend machinery life, reduce maintenance costs, and achieve optimum machinery reliability. This essential text clearly describes the reliability improvement and failure avoidance steps practiced by best-of-class process plants in the U.S. and Europe.

Pumping machinery theory and practice

CERN Document Server

Badr, Hassan M

2014-01-01

Pumping Machinery Theory and Practice comprehensively covers the theoretical foundation and applications of pumping machinery. Key features: Covers characteristics of centrifugal pumps, axial flow pumps and displacement pumpsConsiders pumping machinery performance and operational-type problemsCovers advanced topics in pumping machinery including multiphase flow principles, and two and three-phase flow pumping systemsCovers different methods of flow rate control and relevance to machine efficiency and energy consumptionCovers different methods of flow rate control and relevance to machine effi

Matrix analysis of electrical machinery

CERN Document Server

Hancock, N N

2013-01-01

Matrix Analysis of Electrical Machinery, Second Edition is a 14-chapter edition that covers the systematic analysis of electrical machinery performance. This edition discusses the principles of various mathematical operations and their application to electrical machinery performance calculations. The introductory chapters deal with the matrix representation of algebraic equations and their application to static electrical networks. The following chapters describe the fundamentals of different transformers and rotating machines and present torque analysis in terms of the currents based on the p

46 CFR 58.01-50 - Machinery space, noise.

Science.gov (United States)

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Machinery space, noise. 58.01-50 Section 58.01-50... MACHINERY AND RELATED SYSTEMS General Requirements § 58.01-50 Machinery space, noise. (a) Each machinery space must be designed to minimize the exposure of personnel to noise in accordance with IMO A.468(XII...

Cavitation in Hydraulic Machinery

Energy Technology Data Exchange (ETDEWEB)

Kjeldsen, M.

1996-11-01

The main purpose of this doctoral thesis on cavitation in hydraulic machinery is to change focus towards the coupling of non-stationary flow phenomena and cavitation. It is argued that, in addition to turbulence, superimposed sound pressure fluctuations can have a major impact on cavitation and lead to particularly severe erosion. For the design of hydraulic devices this finding may indicate how to further limit the cavitation problems. Chapter 1 reviews cavitation in general in the context of hydraulic machinery, emphasizing the initial cavitation event and the role of the water quality. Chapter 2 discusses the existence of pressure fluctuations for situations common in such machinery. Chapter 3 on cavitation dynamics presents an algorithm for calculating the nucleation of a cavity cluster. Chapter 4 describes the equipment used in this work. 53 refs., 55 figs.,10 tabs.

29 CFR 1910.215 - Abrasive wheel machinery.

Science.gov (United States)

2010-07-01

... be securely fastened to the spindle and the bearing surface shall run true. When more than one wheel... 29 Labor 5 2010-07-01 2010-07-01 false Abrasive wheel machinery. 1910.215 Section 1910.215 Labor... OCCUPATIONAL SAFETY AND HEALTH STANDARDS Machinery and Machine Guarding § 1910.215 Abrasive wheel machinery. (a...

Strategies for Improving Enterprise Standardization Management of Tropical Crop Machinery

Institute of Scientific and Technical Information of China (English)

无

2007-01-01

@@ There are two categories of tropical crop machinery. One comprises operation machinery that is used for planting, managing and harvesting tropical crops, while the other comprises process machinery for processing tropical crops. Tropical crop machinery is distinguished from other agricultural machinery by the special crops that such machinery cultivates and processes.

FY 1998 survey report. Industrial technology history/succession survey (Survey of originality and creativity of machinery technology of the Japanese industry); 1998 nendo chosa hokokusho. Sangyo gijutsu rekishi keisho chosa (kokunai sangyo kikai gijutsu no dokusosei to sozosei no chosahen)

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

From a viewpoint of long-term development/creative R and D promotion of the industrial technology of Japan, this survey clarifies where there are originality/creativity for technological innovations of Japan and contributes to selecting future technical themes and working out developmental methods. Aiming at succeeding to the industrial technology, this report arranges the results of the examinational research on machinery technology legacy conducted in FY 1998. The total number of registration is 530. The items of classification are as follows: machine tool (cutting processing, plastic processing), power/energy machine (external combustion engine, internal combustion engine, fluid machine, generator/motor), traffic machine (automobile, rolling stock, ship, aircraft, bicycle), industrial machine 1 (iron/steel-making machine, casting machine, mining machine, food producing machine, woodworking machine, textile machine, paper-making machine, printing machine, chemical machine), industrial machine 2 (agriculture/forestry/fisheries machine, civil engineering/construction machine, medical machine, ceramics machine), measuring device/experimental equipment (measuring device, experimental device, design device, machine watch/calculator), machine structure (plant, bridge), etc. (NEDO)

On Development of Agricultural Machinery Operating Service in Chongqing

Institute of Scientific and Technical Information of China (English)

Chongjing; TAN; Shi; YANG

2015-01-01

Development of agricultural machinery operating service in Chongqing takes on rapid increase in number of service organizations,diversified service methods,improvement in service level,and constant service income. However,there are some problems,including unreasonable composition and small scale of service organization,imbalanced development of four service methods,low service level,and low operating income of agricultural machinery households. To accelerate development of agricultural machinery operating service in Chongqing,it is recommended to take following measures: adjusting subsidy for purchase and operation of agricultural machinery; improving fiscal and taxation and financial system; speeding up infrastructure construction,establishing agricultural machinery information network,and improving organizational form and methods of agricultural machinery operating service.

Machinery Vibration Monitoring Program at the Savannah River Site

International Nuclear Information System (INIS)

Potvin, M.M.

1990-01-01

The Reactor Maintenance's Machinery Vibration Monitoring Program (MVMP) plays an essential role in ensuring the safe operation of the three Production Reactors at the Westinghouse Savannah River Company (WRSC) Savannah River Site (SRS). This program has increased machinery availability and reduced maintenance cost by the early detection and determination of machinery problems. This paper presents the Reactor Maintenance's Machinery Vibration Monitoring Program, which has been documented based on Electric Power Research Institute's (EPRI) NP-5311, Utility Machinery Monitoring Guide, and some examples of the successes that it has enjoyed

46 CFR 252.33 - Hull and machinery insurance.

Science.gov (United States)

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Hull and machinery insurance. 252.33 Section 252.33... Subsidy Rates § 252.33 Hull and machinery insurance. (a) Subsidy items. The fair and reasonable net premium costs (including stamp taxes) of hull and machinery, increased value, excess general average...

46 CFR 282.23 - Hull and machinery insurance.

Science.gov (United States)

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Hull and machinery insurance. 282.23 Section 282.23... COMMERCE OF THE UNITED STATES Calculation of Subsidy Rates § 282.23 Hull and machinery insurance. (a) Subsidy items. The fair and reasonable net premium costs (including stamp taxes) of hull and machinery...

Bacterial mitotic machineries

DEFF Research Database (Denmark)

Gerdes, Kenn; Møller-Jensen, Jakob; Ebersbach, Gitte

2004-01-01

Here, we review recent progress that yields fundamental new insight into the molecular mechanisms behind plasmid and chromosome segregation in prokaryotic cells. In particular, we describe how prokaryotic actin homologs form mitotic machineries that segregate DNA before cell division. Thus, the P......M protein of plasmid R1 forms F actin-like filaments that separate and move plasmid DNA from mid-cell to the cell poles. Evidence from three different laboratories indicate that the morphogenetic MreB protein may be involved in segregation of the bacterial chromosome.......Here, we review recent progress that yields fundamental new insight into the molecular mechanisms behind plasmid and chromosome segregation in prokaryotic cells. In particular, we describe how prokaryotic actin homologs form mitotic machineries that segregate DNA before cell division. Thus, the Par...

46 CFR 97.30-5 - Accidents to machinery.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Accidents to machinery. 97.30-5 Section 97.30-5 Shipping... Reports of Accidents, Repairs, and Unsafe Equipment § 97.30-5 Accidents to machinery. (a) In the event of an accident to a boiler, unfired pressure vessel, or machinery tending to render the further use of...

46 CFR 196.30-5 - Accidents to machinery.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Accidents to machinery. 196.30-5 Section 196.30-5... Reports of Accidents, Repairs, and Unsafe Equipment § 196.30-5 Accidents to machinery. (a) In the event of an accident to a boiler, unfired pressure vessel, or machinery tending to render the further use of...

46 CFR 109.205 - Inspection of boilers and machinery.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Inspection of boilers and machinery. 109.205 Section 109... OPERATIONS Tests, Drills, and Inspections § 109.205 Inspection of boilers and machinery. The chief engineer or engineer in charge, before he assumes charge of the boilers and machinery of a unit shall inspect...

46 CFR 185.208 - Accidents to machinery.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Accidents to machinery. 185.208 Section 185.208 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) OPERATIONS Marine Casualties and Voyage Records § 185.208 Accidents to machinery. The owner, managing...

46 CFR 122.208 - Accidents to machinery.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Accidents to machinery. 122.208 Section 122.208 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING MORE THAN 150... Voyage Records § 122.208 Accidents to machinery. The owner, managing operator, or master shall report...

29 CFR 1915.164 - Ship's propulsion machinery.

Science.gov (United States)

2010-07-01

... 29 Labor 7 2010-07-01 2010-07-01 false Ship's propulsion machinery. 1915.164 Section 1915.164 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) OCCUPATIONAL SAFETY AND HEALTH STANDARDS FOR SHIPYARD EMPLOYMENT Ship's Machinery and Piping Systems § 1915.164 Ship's...

Chronic sinusitis and woodworking as risk factors for cancer of the maxillary sinus in northeast Japan.

Science.gov (United States)

Shimizu, H; Hozawa, J; Saito, H; Murai, K; Hirata, H; Takasaka, T; Togawa, K; Konno, A; Kimura, Y; Kikuchi, A

1989-01-01

In the period 1983 to 1985, 66 patients presented to six Japanese university hospitals with squamous cell carcinoma of the maxillary sinus. Using self-administered questionnaires, a case-control study was conducted to examine history of nasal diseases, occupational exposures, and other possible risk factors for this disease. For each patient, two controls were selected from the general population, matched to the patient by sex, age (+/- 5 years), and district of residence. A history of chronic sinusitis was associated with a 2.3-fold increase in risk (p = 0.05). A high relative risk was also observed in males with an occupational history of woodworking or joinery, particularly when these jobs involved sanding or lathing practices (RR = 7.5, p = 0.02). No association between cigarette smoking and maxillary sinus cancer was observed in this study and no evidence was found that indoor air pollution in the home is involved in cancer development.

Vibration vector monitoring of rotating machinery: A predictive/preventative maintenance technique

International Nuclear Information System (INIS)

Humes, B.R.

1990-01-01

Monitoring of overall vibration amplitudes to indicate machinery faults is a standard practice in most industries. The appearance of shaft cracks in machines retrofitted for extended life have prompted development of higher levels of machinery monitoring. Part 1 of this paper discusses vibration vector monitoring for machinery malfunction prediction and failure prevention. Machinery faults which can be diagnosed by this type of monitoring, such as rotor rubs, loose parts, shaft cracks, ..., are presented along with their most common characteristics. The newest, most effective methods of permanent machinery monitoring are presented and critiqued. An extensive case history is presented in Part 2 in which a potentially disastrous machinery fault was predicted using vibration vector monitoring and analysis. The addition of vector monitoring to the normal, overall vibration monitoring proved more effective in diagnosing the machinery fault and predicting impending failure

Accelerating the Transformation of Economic Development Mode to Promote the Sable and Rapid Development of Industry%加快转变经济发展方式 推进行业平稳较快发展

Institute of Scientific and Technical Information of China (English)

张新民

2012-01-01

It is pointed out through the interpretation of the ＂Twelfth Five-Year Plan＂ of the woodworking machinery industry that the next five years is a very import period when the transformation development of woodworking machinery industry and the breakthrough development of industry transfer should be accelerated, structural adjustment and technical innovation should serve as important support for the development of woodworking machinery industry in the following five years , the change in the marked demand should be followed closely to realize the shift to new industries featuring structural optimization, advanced technology, cleanness and safety and high added value, enterprise famous brands should be expanded, strong alliance between enterprises should be promoted in order to be bigger and stronger and great importance should be attached to the soft strength construction of enterprises in terms of human resources, corporate culture and internal management to realize the long-term development of enterprises and enhance the intangible wealth for enterprise development.%对木工机械行业“十二五”规划进行解读，指出未来五年是加快木机行业转型发展、产业转移突破发展的关键五年，要坚持把结构调整和科技创新作为未来五年木机行业发展的重要支撑，要紧跟市场需求变化。向结构优化、技术先进、清洁安全、附加值高的新型产业转向，要扩大企业知名品牌，推进强强联合，做大做强，要重视企业的人力资源、企业文化、内部管理等软实力建设，增加企业发展的无形财富，实现企业长久发展。

Risk assessments and safe machinery ensuring compliance with the EU directives

CERN Document Server

Jespen, Torben

2016-01-01

This book describes the prerequisites for the placing on the market and the safe use of machinery in compliance with the relevant EU Directives, especially the Machinery Directive 2006/42. It provides readers with high-level knowledge concerning the Essential Health and Safety Requirements (EHSR) that machinery must fulfill. The approach and principles of the Machinery Directive were most recently made worldwide acknowledged in the ILO code of practice on safe machinery, released in 2013. The book addresses that code, as well as providing valuable insight into other EU Product and Workplace legislation. Focusing on the key aspect of safe machinery, the “machinery safety risk assessment”, which allows readers to better understand the more difficult aspects of risk assessments, the book equips readers to tackle problems at the manufacturing stage and in different use scenarios, introducing them to risk reduction techniques and functional safety aspects.

46 CFR 182.465 - Ventilation of spaces containing diesel machinery.

Science.gov (United States)

2010-10-01

... furnish natural or powered supply and exhaust ventilation. The total inlet area and the total outlet area... 46 Shipping 7 2010-10-01 2010-10-01 false Ventilation of spaces containing diesel machinery. 182... Ventilation of spaces containing diesel machinery. (a) A space containing diesel machinery must be fitted with...

The Research of Computer Aided Farm Machinery Designing Method Based on Ergonomics

Science.gov (United States)

Gao, Xiyin; Li, Xinling; Song, Qiang; Zheng, Ying

Along with agricultural economy development, the farm machinery product type Increases gradually, the ergonomics question is also getting more and more prominent. The widespread application of computer aided machinery design makes it possible that farm machinery design is intuitive, flexible and convenient. At present, because the developed computer aided ergonomics software has not suitable human body database, which is needed in view of farm machinery design in China, the farm machinery design have deviation in ergonomics analysis. This article puts forward that using the open database interface procedure in CATIA to establish human body database which aims at the farm machinery design, and reading the human body data to ergonomics module of CATIA can product practical application virtual body, using human posture analysis and human activity analysis module to analysis the ergonomics in farm machinery, thus computer aided farm machinery designing method based on engineering can be realized.

46 CFR 97.15-15 - Examination of boilers and machinery.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Examination of boilers and machinery. 97.15-15 Section... VESSELS OPERATIONS Tests, Drills, and Inspections § 97.15-15 Examination of boilers and machinery. It shall be the duty of the chief engineer when assuming charge of the boilers and machinery of a vessel to...

46 CFR 32.35-1 - Boilers and machinery-TB/ALL.

Science.gov (United States)

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Boilers and machinery-TB/ALL. 32.35-1 Section 32.35-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL EQUIPMENT, MACHINERY, AND HULL REQUIREMENTS Main and Auxiliary Machinery § 32.35-1 Boilers and machinery—TB/ALL. Boilers, main and auxiliary...

46 CFR 78.17-30 - Examination of boilers and machinery.

Science.gov (United States)

2010-10-01

... 46 Shipping 3 2010-10-01 2010-10-01 false Examination of boilers and machinery. 78.17-30 Section... OPERATIONS Tests, Drills, and Inspections § 78.17-30 Examination of boilers and machinery. It shall be the duty of the chief engineer when assuming charge of the boilers and machinery of a vessel to examine...

Machinery condition monitoring principles and practices

CERN Document Server

Mohanty, Amiya Ranjan

2015-01-01

Find the Fault in the MachinesDrawing on the author's more than two decades of experience with machinery condition monitoring and consulting for industries in India and abroad, Machinery Condition Monitoring: Principles and Practices introduces the practicing engineer to the techniques used to effectively detect and diagnose faults in machines. Providing the working principle behind the instruments, the important elements of machines as well as the technique to understand their conditions, this text presents every available method of machine fault detection occurring in machines in general, an

46 CFR 196.15-15 - Examination of boilers and machinery.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Examination of boilers and machinery. 196.15-15 Section... VESSELS OPERATIONS Test, Drills, and Inspections § 196.15-15 Examination of boilers and machinery. (a) It shall be the duty of the chief engineer when he assumes charge of the boilers and machinery of a vessel...

Vibration of hydraulic machinery

CERN Document Server

Wu, Yulin; Liu, Shuhong; Dou, Hua-Shu; Qian, Zhongdong

2013-01-01

Vibration of Hydraulic Machinery deals with the vibration problem which has significant influence on the safety and reliable operation of hydraulic machinery. It provides new achievements and the latest developments in these areas, even in the basic areas of this subject. The present book covers the fundamentals of mechanical vibration and rotordynamics as well as their main numerical models and analysis methods for the vibration prediction. The mechanical and hydraulic excitations to the vibration are analyzed, and the pressure fluctuations induced by the unsteady turbulent flow is predicted in order to obtain the unsteady loads. This book also discusses the loads, constraint conditions and the elastic and damping characters of the mechanical system, the structure dynamic analysis, the rotor dynamic analysis and the system instability of hydraulic machines, including the illustration of monitoring system for the instability and the vibration in hydraulic units. All the problems are necessary for vibration pr...

46 CFR 78.33-5 - Accidents to machinery.

Science.gov (United States)

2010-10-01

... 46 Shipping 3 2010-10-01 2010-10-01 false Accidents to machinery. 78.33-5 Section 78.33-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS OPERATIONS Reports of Accidents, Repairs, and Unsafe Equipment § 78.33-5 Accidents to machinery. (a) In the event of an accident...

Open innovation and supply chain management in food machinery ...

African Journals Online (AJOL)

Open innovation and supply chain management in food machinery supply chain: a ... This paradigm describes a new approach to internal R&D management, which ... a picture of the adoption of open innovation in the food machinery industry.

46 CFR 169.629 - Compartments containing gasoline machinery or fuel tanks.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Compartments containing gasoline machinery or fuel tanks. 169.629 Section 169.629 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL... gasoline machinery or fuel tanks. Spaces containing gasoline machinery or fuel tanks must have natural...

46 CFR 116.620 - Ventilation of machinery and fuel tank spaces.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Ventilation of machinery and fuel tank spaces. 116.620... AND ARRANGEMENT Ventilation § 116.620 Ventilation of machinery and fuel tank spaces. In addition to the requirements of this subpart, ventilation systems for spaces containing machinery or fuel tanks...

46 CFR 91.15-1 - Standards in inspection of hulls, boilers, and machinery.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Standards in inspection of hulls, boilers, and machinery... hulls, boilers, and machinery. In the inspection of hulls, boilers, and machinery of vessels, the..., respecting material and inspection of hulls, boilers, and machinery, and the certificate of classification...

46 CFR 189.15-1 - Standards in inspection of hulls, boilers, and machinery.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Standards in inspection of hulls, boilers, and machinery... inspection of hulls, boilers, and machinery. In the inspection of hulls, boilers, and machinery of vessels... chapter, respecting material and construction of hulls, boilers, and machinery, and certificate of...

46 CFR 71.15-1 - Standards in inspection of hulls, boilers, and machinery.

Science.gov (United States)

2010-10-01

... 46 Shipping 3 2010-10-01 2010-10-01 false Standards in inspection of hulls, boilers, and machinery..., boilers, and machinery. In the inspection of hulls, boilers, and machinery of vessels, the standards... and inspection of hulls, boilers, and machinery, and the certificate of classification referring...

49 CFR 1242.47 - Machinery (account XX-27-40).

Science.gov (United States)

2010-10-01

... 49 Transportation 9 2010-10-01 2010-10-01 false Machinery (account XX-27-40). 1242.47 Section 1242...-Equipment § 1242.47 Machinery (account XX-27-40). Separate common expenses on the basis of the freight/passenger separation of administration (account XX-27-01). ...

46 CFR 169.315 - Ventilation (other than machinery spaces).

Science.gov (United States)

2010-10-01

... section is satisfied, a vessel having only a natural ventilation system must satisfy the following: V/A≥1... 46 Shipping 7 2010-10-01 2010-10-01 false Ventilation (other than machinery spaces). 169.315... SCHOOL VESSELS Construction and Arrangement Hull Structure § 169.315 Ventilation (other than machinery...

40 CFR 180.521 - Fumigants for grain-mill machinery; tolerances for residues.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Fumigants for grain-mill machinery... Tolerances § 180.521 Fumigants for grain-mill machinery; tolerances for residues. (a) General. Fumigants may be safely used in or on grain-mill machinery in accordance with the following prescribed conditions...

Genome-scale modeling of the protein secretory machinery in yeast

DEFF Research Database (Denmark)

Feizi, Amir; Österlund, Tobias; Petranovic, Dina

2013-01-01

The protein secretory machinery in Eukarya is involved in post-translational modification (PTMs) and sorting of the secretory and many transmembrane proteins. While the secretory machinery has been well-studied using classic reductionist approaches, a holistic view of its complex nature is lacking....... Here, we present the first genome-scale model for the yeast secretory machinery which captures the knowledge generated through more than 50 years of research. The model is based on the concept of a Protein Specific Information Matrix (PSIM: characterized by seven PTMs features). An algorithm...

Machinery failure analysis and troubleshooting practical machinery management for process plants

CERN Document Server

Bloch, Heinz P

2012-01-01

Solve the machinery failure problems costing you time and money with this classic, comprehensive guide to analysis and troubleshooting  Provides detailed, complete and accurate information on anticipating risk of component failure and avoiding equipment downtime Includes numerous photographs of failed parts to ensure you are familiar with the visual evidence you need to recognize Covers proven approaches to failure definition and offers failure identification and analysis methods that can be applied to virtually all problem situations Demonstr

Radiation technologies in metallurgy and machinery

International Nuclear Information System (INIS)

Meshkov, I.N.

1990-01-01

Applications of electron beam accelerators for technologies in metallurgy and machinery are discussed. Processes described are provided with special industrial accelerators, developed in the Institute of Nuclear Physics, Novosibirsk. (author)

FOREWORD: 26th IAHR Symposium on Hydraulic Machinery and Systems

Science.gov (United States)

Wu, Yulin; Wang, Zhengwei; Liu, Shuhong; Yuan, Shouqi; Luo, Xingqi; Wang, Fujun

2012-11-01

The 26th IAHR Symposium on Hydraulic Machinery and Systems, will be held in Beijing, China, 19-23 August 2012. It is jointly organized by Tsinghua University, State Key Laboratory of Hydro Science and Hydraulic Engineering, China, Jiangsu University, Xi'an University of Technology, China Agricultural University, National Engineering Research Center of Hydropower Equipment and Dongfang Electric Machinery Co., Ltd. It is the second time that China hosts such a symposium. By the end of 2011, the China electrical power system had a total of 1 050 GW installed power, out of which 220 GW was in hydropower plants. The energy produced in hydropower facilities was 662.6 TWh from a total of 4,720 TWh electrical energy production in 2011. Moreover, in 2020, new hydropower capacities are going to be developed, with a total of 180 GW installed power and an estimated 708 TWh/year energy production. And in 2011, the installed power of pumped storage stations was about 25GW. In 2020, the data will be 70GW. At the same time, the number of pumps used in China is increasing rapidly. China produces about 29,000,000 pumps with more than 220 series per year. By the end of 2011, the Chinese pumping system has a total of 950 GW installed power. The energy consumed in pumping facilities was 530 TWh in 2011. The pump energy consumption accounted for about 12% of the national electrical energy production. Therefore, there is a large market in the field of hydraulic machinery including water turbines, pump turbines and a variety of pumps in China. There are also many research projects in this field. For example, we have conducted National Key Research Projects on 1000 MW hydraulic turbine, and on the pump turbines with high head, as well as on the large capacity pumps for water supply. Tsinghua University of Beijing is proud to host the 26th IAHR Symposium on Hydraulic Machinery and Systems. Tsinghua University was established in 1911, after the founding of the People's Republic of China. It

Fault size classification of rotating machinery using support vector machine

Energy Technology Data Exchange (ETDEWEB)

Kim, Y. S.; Lee, D. H.; Park, S. K. [Korea Hydro and Nuclear Power Co. Ltd., Daejeon (Korea, Republic of)

2012-03-15

Studies on fault diagnosis of rotating machinery have been carried out to obtain a machinery condition in two ways. First is a classical approach based on signal processing and analysis using vibration and acoustic signals. Second is to use artificial intelligence techniques to classify machinery conditions into normal or one of the pre-determined fault conditions. Support Vector Machine (SVM) is well known as intelligent classifier with robust generalization ability. In this study, a two-step approach is proposed to predict fault types and fault sizes of rotating machinery in nuclear power plants using multi-class SVM technique. The model firstly classifies normal and 12 fault types and then identifies their sizes in case of predicting any faults. The time and frequency domain features are extracted from the measured vibration signals and used as input to SVM. A test rig is used to simulate normal and the well-know 12 artificial fault conditions with three to six fault sizes of rotating machinery. The application results to the test data show that the present method can estimate fault types as well as fault sizes with high accuracy for bearing an shaft-related faults and misalignment. Further research, however, is required to identify fault size in case of unbalance, rubbing, looseness, and coupling-related faults.

Fault size classification of rotating machinery using support vector machine

International Nuclear Information System (INIS)

Kim, Y. S.; Lee, D. H.; Park, S. K.

2012-01-01

Studies on fault diagnosis of rotating machinery have been carried out to obtain a machinery condition in two ways. First is a classical approach based on signal processing and analysis using vibration and acoustic signals. Second is to use artificial intelligence techniques to classify machinery conditions into normal or one of the pre-determined fault conditions. Support Vector Machine (SVM) is well known as intelligent classifier with robust generalization ability. In this study, a two-step approach is proposed to predict fault types and fault sizes of rotating machinery in nuclear power plants using multi-class SVM technique. The model firstly classifies normal and 12 fault types and then identifies their sizes in case of predicting any faults. The time and frequency domain features are extracted from the measured vibration signals and used as input to SVM. A test rig is used to simulate normal and the well-know 12 artificial fault conditions with three to six fault sizes of rotating machinery. The application results to the test data show that the present method can estimate fault types as well as fault sizes with high accuracy for bearing an shaft-related faults and misalignment. Further research, however, is required to identify fault size in case of unbalance, rubbing, looseness, and coupling-related faults

The Autophagic Machinery in Enterovirus Infection.

Science.gov (United States)

Lai, Jeffrey K F; Sam, I-Ching; Chan, Yoke Fun

2016-01-27

The Enterovirus genus of the Picornaviridae family comprises many important human pathogens, including polioviruses, rhinovirus, enterovirus A71, and enterovirus D68. They cause a wide variety of diseases, ranging from mild to severe life-threatening diseases. Currently, no effective vaccine is available against enteroviruses except for poliovirus. Enteroviruses subvert the autophagic machinery to benefit their assembly, maturation, and exit from host. Some enteroviruses spread between cells via a process described as autophagosome-mediated exit without lysis (AWOL). The early and late phases of autophagy are regulated through various lipids and their metabolizing enzymes. Some of these lipids and enzymes are specifically regulated by enteroviruses. In the present review, we summarize the current understanding of the regulation of autophagic machinery by enteroviruses, and provide updates on recent developments in this field.

A Survey of Fish Production and Processing Machinery in Rivers ...

African Journals Online (AJOL)

Survey of fish production and processing machinery in Port Harcourt City Local Government Area of Rivers State, Nigeria was carried out to evaluate the followings: different machines used for fish production and processing, the most acceptable machine, effect of cost of machinery on the fish farmer, whether gender has ...

Detection of Early Faults in Rotating Machinery Based on Wavelet Analysis

Directory of Open Access Journals (Sweden)

Meng Hee Lim

2013-01-01

Full Text Available This paper explores the application of wavelet analysis for the detection of early changes in rotor dynamics caused by common machinery faults, namely, rotor unbalance and minor blade rubbing conditions. In this paper, the time synchronised wavelet analysis method was formulated and its effectiveness to detect machinery faults at the early stage was evaluated based on signal simulation and experimental study. The proposed method provides a more standardised approach to visualise the current state of rotor dynamics of a rotating machinery by taking into account the effects of time shift, wavelet edge distortion, and system noise suppression. The experimental results showed that this method is able to reveal subtle changes of the vibration signal characteristics in both the frequency content distribution and the amplitude distortion caused by minor rotor unbalance and blade rubbing conditions. Besides, this method also appeared to be an effective tool to diagnose and to discriminate the different types of machinery faults based on the unique pattern of the wavelet contours. This study shows that the proposed wavelet analysis method is promising to reveal machinery faults at early stage as compared to vibration spectrum analysis.

BIOMECHANIC EVALUATION OF CARPENTRY WORKERS IN THE DISTRITO FEDERAL, BRAZIL

Directory of Open Access Journals (Sweden)

Nilton Cesar Fiedler

2010-08-01

Full Text Available The aim of this study was the biomechanical assessment of carpentry woodworkers, located in Brasília, DF. It was filmed the profile of each worker during the performance of his activities in the carpentry and the forces involved in the work were assessed. The image of each woodworker was congealed to accomplish the measurement of articulation angles. The data were submitted to the software of posture analysis “Winowas” (OWAS Method and to the biomechanic model of posture prognosis and static forces, developed by Michigan University. The OWAS method showed that, for all machines and carpentries assessed, the worst posture occurred when the worker lifted and placed the pieces of wood on the floor and during the feeding in the smoother. The tridimensional biomechanic model registered the worst posture in different phases of the work cycle. In the first one, there were problems in all articulations, except the hips, when placing the pieces on the floor from the smoother. In the second one, there were problems in all articulations, except the elbows and the L5-S1 column disc, by feeding the surface planer. The third one, the ankles were the most injured when feeding the smoother, the surface planer, the circular saw and the band saw. According to the results, the woodworkers should try to eliminate the constant work standing upright, use auxiliary machinery to handle pieces of wood, reduce the load during feeding the machines and improve postures.

The capacity of sugar beet farms’ machinery and equipment

Directory of Open Access Journals (Sweden)

Małgorzata BZOWSKA – BAKALARZ

2012-12-01

Full Text Available The survey investigates into equipment of sugar beet farms of the Lublin region, Poland, with machinery – with reference to plantation size and yields. To assess the production potential of the farms, the authors determined the age structure of the machinery owned by the farmers and established the scale of investment in new equipment. The machinery most important for sugar beet production are pre-sowing and post-harvest tillage units, sprayers, seed drills, combine harvesters and self-unloading trailers. In most cases, the surveyed farmers own most of these machines, but they are often obsolete: 37% of them is in operation for more than 15 years. As for the machines dedicated solely to sugar beet growing (harvesters and seeders, their age structure is most unfavourable – 70% of them have been used for over 15 years. A trend towards increasing plantation sizes provides incentives for introducing innovation to cultivation methods. However, the scale of investment in new machinery is small, especially in the case of small and medium-sized farms that dominate in the region. The authors surveyed also the scale of using professional services in the field of tillage processes to determine changes in farming practices.

Multiscale singular value manifold for rotating machinery fault diagnosis

Energy Technology Data Exchange (ETDEWEB)

Feng, Yi; Lu, BaoChun; Zhang, Deng Feng [School of Mechanical Engineering, Nanjing University of Science and Technology,Nanjing (United States)

2017-01-15

Time-frequency distribution of vibration signal can be considered as an image that contains more information than signal in time domain. Manifold learning is a novel theory for image recognition that can be also applied to rotating machinery fault pattern recognition based on time-frequency distributions. However, the vibration signal of rotating machinery in fault condition contains cyclical transient impulses with different phrases which are detrimental to image recognition for time-frequency distribution. To eliminate the effects of phase differences and extract the inherent features of time-frequency distributions, a multiscale singular value manifold method is proposed. The obtained low-dimensional multiscale singular value manifold features can reveal the differences of different fault patterns and they are applicable to classification and diagnosis. Experimental verification proves that the performance of the proposed method is superior in rotating machinery fault diagnosis.

STUDY OF THE PARAMETERS OF EFFICIENCY IN CENTRES FOR REPAIR OF AGRICULTURAL MACHINERY

Directory of Open Access Journals (Sweden)

Natalia Stoyanova

2015-06-01

Full Text Available The paper makes a thorough study of the parameters of efficiency in the centers for the repair of agricultural machinery, considering production and technological structure, the basic principles for design of the process of service, the quantitative indicators for servicing. It presents a theoretical model for the management of services in the service business, taking into account the basic system requirements for maintenance of agricultural machinery, the main elements of the standards of customer service, choice of forms for maintenance of agricultural machinery. Opportunities are proposed for the optimization of repair activities in the centers for repair of agricultural machinery.

Condition Monitoring of Machinery in Non-Stationary Operations : Proceedings of the Second International Conference "Condition Monitoring of Machinery in Non-Stationnary Operations"

CERN Document Server

Bartelmus, Walter; Chaari, Fakher; Zimroz, Radoslaw; Haddar, Mohamed

2012-01-01

Condition monitoring of machines in non-stationary operations (CMMNO) can be seen as the major challenge for research in the field of machinery diagnostics. Condition monitoring of machines in non-stationary operations is the title of the presented book and the title of the Conference held in Hammamet - Tunisia March 26 – 28, 2012. It is the second conference under this title, first took place in Wroclaw - Poland , March 2011. The subject CMMNO comes directly from industry needs and observation of real objects. Most monitored and diagnosed objects used in industry works in non-stationary operations condition. The non-stationary operations come from fulfillment of machinery tasks, for which they are designed for. All machinery used in different kind of mines, transport systems, vehicles like: cars, buses etc, helicopters, ships and battleships and so on work in non-stationary operations. The papers included in the book are shaped by the organizing board of the conference and authors of the papers. The papers...

Design and Realization of Rotating Machinery Conditions Monitoring System Based on Labview

Science.gov (United States)

Fan, Qiyuan

Nonlinear dynamic analysis of rotating machinery system has always been the hot spot of the rotational dynamics research. This article sets up a rotating machinery condition monitoring system to realize the measurement of system dynamic characteristic parameters based on NI(National Instruments) virtual instruments technology. The measurement of vibration signal of rotating machinery system is achieved by using NI company general data acquisition module of NI company. Meanwhile, by analyzing and processing the acquired data using Labview 2012, the dynamic characteristics, such as .the speed of the rotating machinery system, the axis trajectory, spectrum parameters, are attained. The measurement results show that the rotating machinery condition monitoring system based on Labview is easy to operate, easy to realize the function extension and maintenance, and that it can be used in the industrial engineering projects with rotation characteristics. Labview as the development tools used by virtual instrument function, is very powerful data acquisition software products support is one of the features of it, so using Labview programming and data acquisition is simple and convenient [1].

Emergence and modular evolution of a novel motility machinery in bacteria.

Directory of Open Access Journals (Sweden)

Jennifer Luciano

2011-09-01

Full Text Available Bacteria glide across solid surfaces by mechanisms that have remained largely mysterious despite decades of research. In the deltaproteobacterium Myxococcus xanthus, this locomotion allows the formation stress-resistant fruiting bodies where sporulation takes place. However, despite the large number of genes identified as important for gliding, no specific machinery has been identified so far, hampering in-depth investigations. Based on the premise that components of the gliding machinery must have co-evolved and encode both envelope-spanning proteins and a molecular motor, we re-annotated known gliding motility genes and examined their taxonomic distribution, genomic localization, and phylogeny. We successfully delineated three functionally related genetic clusters, which we proved experimentally carry genes encoding the basal gliding machinery in M. xanthus, using genetic and localization techniques. For the first time, this study identifies structural gliding motility genes in the Myxobacteria and opens new perspectives to study the motility mechanism. Furthermore, phylogenomics provide insight into how this machinery emerged from an ancestral conserved core of genes of unknown function that evolved to gliding by the recruitment of functional modules in Myxococcales. Surprisingly, this motility machinery appears to be highly related to a sporulation system, underscoring unsuspected common mechanisms in these apparently distinct morphogenic phenomena.

46 CFR 169.631 - Separation of machinery and fuel tank spaces from accommodation spaces.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Separation of machinery and fuel tank spaces from accommodation spaces. 169.631 Section 169.631 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED... machinery and fuel tank spaces from accommodation spaces. (a) Machinery and fuel tank spaces must be...

Application of DNA Machineries for the Barcode Patterned Detection of Genes or Proteins.

Science.gov (United States)

Zhou, Zhixin; Luo, Guofeng; Wulf, Verena; Willner, Itamar

2018-06-05

The study introduces an analytical platform for the detection of genes or aptamer-ligand complexes by nucleic acid barcode patterns generated by DNA machineries. The DNA machineries consist of nucleic acid scaffolds that include specific recognition sites for the different genes or aptamer-ligand analytes. The binding of the analytes to the scaffolds initiate, in the presence of the nucleotide mixture, a cyclic polymerization/nicking machinery that yields displaced strands of variable lengths. The electrophoretic separation of the resulting strands provides barcode patterns for the specific detection of the different analytes. Mixtures of DNA machineries that yield, upon sensing of different genes (or aptamer ligands), one-, two-, or three-band barcode patterns are described. The combination of nucleic acid scaffolds acting, in the presence of polymerase/nicking enzyme and nucleotide mixture, as DNA machineries, that generate multiband barcode patterns provide an analytical platform for the detection of an individual gene out of many possible genes. The diversity of genes (or other analytes) that can be analyzed by the DNA machineries and the barcode patterned imaging is given by the Pascal's triangle. As a proof-of-concept, the detection of one of six genes, that is, TP53, Werner syndrome, Tay-Sachs normal gene, BRCA1, Tay-Sachs mutant gene, and cystic fibrosis disorder gene by six two-band barcode patterns is demonstrated. The advantages and limitations of the detection of analytes by polymerase/nicking DNA machineries that yield barcode patterns as imaging readout signals are discussed.

46 CFR 177.620 - Ventilation of machinery and fuel tank spaces.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Ventilation of machinery and fuel tank spaces. 177.620... fuel tank spaces. In addition to the requirements of this subpart, ventilation systems for spaces containing machinery or fuel tanks must comply with the requirements of part 182 of this chapter. ...

Structural design of nuclear reactor machinery and equipment

International Nuclear Information System (INIS)

Hara, Hideki

1992-01-01

Since the machinery, equipment and piping which compose nuclear power station facilities are diverse, when those are designed, consideration is given sufficiently to the objective of use and the importance of the object machinery and equipment so that those can maintain the soundness over the design life. In this report, on the contents and the design standard in the design techniques for nuclear reactor machinery and equipment, the way of thinking is shown, taking an example of reactor pressure vessel which is stipulated as the vessel kind 1 in the 'Technical standard of structures and others regarding nuclear facilities for electric power generation', Notice No. 501 of the Ministry of International Trade and Industry. The reactor pressure vessel of 1350 MWe improved type BWR (ABWR) is used under the condition of 87.9 kg/cm 2 and 302 degC, and the inside diameter is about 7.2 m, the inside height is about 21 m, and the wall thickness is about 170 mm. The design standard for reactor pressure vessels and its way of thinking, breakdown prevention design and the design techniques for reactor pressure vessels are described. (K.I.)

Targeting Cell Polarity Machinery to Exhaust Breast Cancer Stem Cells

Science.gov (United States)

2017-10-01

AWARD NUMBER: W81XWH-15-1-0644 TITLE: Targeting Cell Polarity Machinery to Exhaust Breast Cancer Stem Cells PRINCIPAL INVESTIGATOR: Chun-Ju...Targeting Cell Polarity Machinery to Exhaust Breast Cancer Stem Cells 5a. CONTRACT NUMBER 5b. GRANT NUMBER W81XWH-15-1-0644 5c. PROGRAM ELEMENT...Unlimited 13. SUPPLEMENTARY NOTES 14. ABSTRACT Cancer stem cells (CSCs), a cell population with acquired perpetuating self-renewal properties which

Fault Diagnosis for Rotating Machinery: A Method based on Image Processing.

Directory of Open Access Journals (Sweden)

Chen Lu

Full Text Available Rotating machinery is one of the most typical types of mechanical equipment and plays a significant role in industrial applications. Condition monitoring and fault diagnosis of rotating machinery has gained wide attention for its significance in preventing catastrophic accident and guaranteeing sufficient maintenance. With the development of science and technology, fault diagnosis methods based on multi-disciplines are becoming the focus in the field of fault diagnosis of rotating machinery. This paper presents a multi-discipline method based on image-processing for fault diagnosis of rotating machinery. Different from traditional analysis method in one-dimensional space, this study employs computing method in the field of image processing to realize automatic feature extraction and fault diagnosis in a two-dimensional space. The proposed method mainly includes the following steps. First, the vibration signal is transformed into a bi-spectrum contour map utilizing bi-spectrum technology, which provides a basis for the following image-based feature extraction. Then, an emerging approach in the field of image processing for feature extraction, speeded-up robust features, is employed to automatically exact fault features from the transformed bi-spectrum contour map and finally form a high-dimensional feature vector. To reduce the dimensionality of the feature vector, thus highlighting main fault features and reducing subsequent computing resources, t-Distributed Stochastic Neighbor Embedding is adopt to reduce the dimensionality of the feature vector. At last, probabilistic neural network is introduced for fault identification. Two typical rotating machinery, axial piston hydraulic pump and self-priming centrifugal pumps, are selected to demonstrate the effectiveness of the proposed method. Results show that the proposed method based on image-processing achieves a high accuracy, thus providing a highly effective means to fault diagnosis for

Fault Diagnosis for Rotating Machinery: A Method based on Image Processing.

Science.gov (United States)

Lu, Chen; Wang, Yang; Ragulskis, Minvydas; Cheng, Yujie

2016-01-01

Rotating machinery is one of the most typical types of mechanical equipment and plays a significant role in industrial applications. Condition monitoring and fault diagnosis of rotating machinery has gained wide attention for its significance in preventing catastrophic accident and guaranteeing sufficient maintenance. With the development of science and technology, fault diagnosis methods based on multi-disciplines are becoming the focus in the field of fault diagnosis of rotating machinery. This paper presents a multi-discipline method based on image-processing for fault diagnosis of rotating machinery. Different from traditional analysis method in one-dimensional space, this study employs computing method in the field of image processing to realize automatic feature extraction and fault diagnosis in a two-dimensional space. The proposed method mainly includes the following steps. First, the vibration signal is transformed into a bi-spectrum contour map utilizing bi-spectrum technology, which provides a basis for the following image-based feature extraction. Then, an emerging approach in the field of image processing for feature extraction, speeded-up robust features, is employed to automatically exact fault features from the transformed bi-spectrum contour map and finally form a high-dimensional feature vector. To reduce the dimensionality of the feature vector, thus highlighting main fault features and reducing subsequent computing resources, t-Distributed Stochastic Neighbor Embedding is adopt to reduce the dimensionality of the feature vector. At last, probabilistic neural network is introduced for fault identification. Two typical rotating machinery, axial piston hydraulic pump and self-priming centrifugal pumps, are selected to demonstrate the effectiveness of the proposed method. Results show that the proposed method based on image-processing achieves a high accuracy, thus providing a highly effective means to fault diagnosis for rotating machinery.

Modern principles used in conformity assessment of machinery from forestry sector

Directory of Open Access Journals (Sweden)

Antonov Anca Elena

2017-01-01

Full Text Available The paper is aiming to implement the general principles of risk prevention at employer’s level, with respect to occupational risks evaluation, the elimination of risk and accident factors, and information of workers which are using the machinery in the forestry sector. For the use of machinery in the forestry sector in terms of economic performance and a level of maximum safety, it is necessary to ensure the user guides set by the manufacturer in terms of commissioning, use and to provide appropriate safe working operations and interventions and to guarantee the technical and environmental requirements, including appropriate measures and means of protection against accidents and occupational disease. The impact of occupational risks for machinery used in this sector can be reduced through the application of modern principles in conformity assessment and certification and, where appropriate, through technical diagnostics and inspection, taking into account the provisions of the new Machinery Directive 2006/42 / EC which is imposing the obligation of manufacturer to implement conformity assessment procedures in accordance with the methods of assessment and verification of safety at the certification bodies, notified at the European Commission. The paper aims to develop modern technical tools for conformity assessment and verification of this category of machines used in the forestry sector that would provide prerequisite for increasing competitiveness of employers in the market economy. Applying these tools of modern technology for manufacturers and users of this category of machinery provides the necessary conditions for placing on the market of safe products with a appropriate safety level, in the intended using conditions, in order to guarantee the essential requirements for safety and health, technical and environmental conditions, including measures and means of protection. The result of this research is to develop technical tools needed to

The accident analysis of mobile mine machinery in Indian opencast coal mines.

Science.gov (United States)

Kumar, R; Ghosh, A K

2014-01-01

This paper presents the analysis of large mining machinery related accidents in Indian opencast coal mines. The trends of coal production, share of mining methods in production, machinery deployment in open cast mines, size and population of machinery, accidents due to machinery, types and causes of accidents have been analysed from the year 1995 to 2008. The scrutiny of accidents during this period reveals that most of the responsible factors are machine reversal, haul road design, human fault, operator's fault, machine fault, visibility and dump design. Considering the types of machines, namely, dumpers, excavators, dozers and loaders together the maximum number of fatal accidents has been caused by operator's faults and human faults jointly during the period from 1995 to 2008. The novel finding of this analysis is that large machines with state-of-the-art safety system did not reduce the fatal accidents in Indian opencast coal mines.

Densification of chipper harvested SRC using on-farm machinery

Energy Technology Data Exchange (ETDEWEB)

Paulson, M.

2003-07-01

This report gives details of a project to density wood chips using on-farm machinery in order to avoid problems encountered in bulk handling and storage of low density short rotation cultivation (SRC) wood chips - especially as some customers can only accept baled material. Trials using different lengths of chips produced by a standard SRC harvester are described, and the failure to produce acceptable bales is reported. The potential cost of modifying equipment is deemed to make the baling of SRC chips using standard farm machinery currently not viable.

49 CFR 393.130 - What are the rules for securing heavy vehicles, equipment and machinery?

Science.gov (United States)

2010-10-01

... heavy vehicles, equipment and machinery? (a) Applicability. The rules in this section apply to the transportation of heavy vehicles, equipment and machinery which operate on wheels or tracks, such as front end... heavy vehicles, equipment or machinery with crawler tracks or wheels. (1) In addition to the...

Intelligence and the machinery of government: conceptualizing the intelligence community

OpenAIRE

Davies, PHJ

2010-01-01

This article argues that the failure to address intelligence agencies as public organizations part and parcel with the overt machinery of government constitutes a significant lacuna both in the specialist study of intelligence and the broader discipline of public administration studies. The role and status of intelligence institutions as aspects of the machinery of central government is examined, along with the prospects of certain key paradigms in the field for understanding those institutio...

Vibration Feature Extraction and Analysis for Fault Diagnosis of Rotating Machinery-A Literature Survey

OpenAIRE

Saleem Riaz; Hassan Elahi; Kashif Javaid; Tufail Shahzad

2017-01-01

Safety, reliability, efficiency and performance of rotating machinery in all industrial applications are the main concerns. Rotating machines are widely used in various industrial applications. Condition monitoring and fault diagnosis of rotating machinery faults are very important and often complex and labor-intensive. Feature extraction techniques play a vital role for a reliable, effective and efficient feature extraction for the diagnosis of rotating machinery. Therefore, deve...

High voltage electrification of tractor and agricultural machinery – A review

International Nuclear Information System (INIS)

Moreda, G.P.; Muñoz-García, M.A.; Barreiro, P.

2016-01-01

Highlights: • CO_2 emission reduction calls for energy-efficient mobile work machines. • Energy recovery could be possible in some agricultural machinery operations. • Owning and operating costs of electrified tractor-implement pairs should be analyzed. • Plug-in hybrid electric tractors running along tramlines make sense. • Electrification allows for precise speed control, noise reduction and flexible design. - Abstract: Reduction of both pollutant emissions and fossil fuel dependency is an objective of energy policies worldwide. In many countries, governments promote the use of efficient vehicles like the hybrid electric vehicle. Incorporation of electric drives in tractor and agricultural machinery presents advantages in terms of increased energy efficiency and expanded functionalities. Higher efficiency means reduction in fuel consumption and subsequent decrease in CO_2 emission. New functionalities improve work quality and increase operator comfort. Tractor electrification takes advantage of decoupling loads and drives from the engine, which allows operating the latter at its highest efficiency point. Major advantages of machinery electrification are torque and speed control, noise reduction, and a more flexible design. In this paper, a review of the state-of-the-art of agricultural machinery high voltage electrification is presented.

CCPIT Machinery Exhibition Succeeded in Kuala Lumpur

Institute of Scientific and Technical Information of China (English)

无

2005-01-01

@@ From August 18 to 20, 2005, China Council for the Promotion of International Trade(CCPIT) held China Machinery and Electronics Trade Exhibition, CME 2005 in Kuala Lumpur, the capital of Malaysia on behalf of China, a good job has been done.

Structural insights into the bacterial carbon - phosphorus lyase machinery

DEFF Research Database (Denmark)

Seweryn, Paulina; Van, Lan Bich; Kjeldgaard, Morten

2015-01-01

Phosphorus is required for all life and microorganisms can extract it from their environment through several metabolic pathways. When phosphate is in limited supply, some bacteria are able to use phosphonate compounds, which require specialized enzymatic machinery to break the stable carbon......–phosphorus (C–P) bond. Despite its importance, the details of how this machinery catabolizes phosphonates remain unknown. Here we determine the crystal structure of the 240-kilodalton Escherichia coli C–P lyase core complex (PhnG–PhnH–PhnI–PhnJ; PhnGHIJ), and show that it is a two-fold symmetric hetero...

THE MEASUREMENT AND DISTRIBUTION OF WOOD DUST

Directory of Open Access Journals (Sweden)

Andrea Rosario Proto

2010-03-01

Full Text Available In Italy, the woodworking industry presents many issues in terms of occupational health and safety. This study on exposure to wood dust could contribute to the realization of a prevention model in order to limit exposure to carcinogenic agents to the worker. The sampling methodology illustrated the analysis of dust emissions from the woodworking machinery in operation throughout the various processing cycles. The quantitative and qualitative assessment of exposure was performed using two different methodologies. The levels of wood dust were determined according to EN indications and sampling was conducted using IOM and Cyclon personal samplers. The qualitative research of wood dust was performed using an advanced laser air particle counter. This allowed the number of particles present to be counted in real time. The results obtained allowed for an accurate assessment of the quality of the dust emitted inside the workplace during the various processing phases. The study highlighted the distribution of air particles within the different size classes, the exact number of both thin and ultra-thin dusts, and confirmed the high concentration of thin dust particles which can be very harmful to humans.

46 CFR 167.65-60 - Examination of boilers and machinery by engineer.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Examination of boilers and machinery by engineer. 167.65-60 Section 167.65-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL... machinery by engineer. It shall be the duty of an engineer when he assumes charge of the boilers and...

CCPIT Machinery Exhibition Succeeded in Kuala Lumpur

Institute of Scientific and Technical Information of China (English)

无

2005-01-01

　　From August 18 to 20, 2005, China Council for the Promotion of International Trade(CCPIT) held China Machinery and Electronics Trade Exhibition, CME 2005 in Kuala Lumpur, the capital of Malaysia on behalf of China, a good job has been done.……

Web-based Interactive Simulator for Rotating Machinery.

Science.gov (United States)

Sirohi, Vijayalaxmi

1999-01-01

Baroma (Balance of Rotating Machinery), the Web-based educational engineering interactive software for teaching/learning combines didactical and software ergonomical approaches. The software in tutorial form simulates a problem using Visual Interactive Simulation in graphic display, and animation is brought about through graphical user interface…

Directional Wigner-Ville distribution and its application for rotating-machinery condition monitoring

International Nuclear Information System (INIS)

Kim, Dong Wan; Ha, Jae HOng; Shin, Hae Gon; Lee, Yoon Hee; Kim, Young Baik

1996-01-01

Vibration analysis is one of the most powerful tools available for the detection and isolation of incipient faults in mechanical systems. The methods of vibration analysis in use today and under continuous study are broad band vibration monitoring, time domain analysis, and frequency domain analysis. In recent years, great interest has been generated concerning the use of time-frequency representation and its application for a machinery diagnostics and condition monitoring system. The objective of the research described in this paper was to develop a new diagnostic tool for the rotating machinery. This paper introduces a new time-frequency representation, Directional Wigner-Ville Distribution, which analyses the time-frequency structure of the rotating machinery vibration

ERGONOMIC AND ECOLOGICALSAFETY - FACTORSNESSARY TO IMPROVETHE COMPETITIVENESS OF THE DOMESTIC CONSTRUCTION MACHINERY

Directory of Open Access Journals (Sweden)

Volkov Andrey Anatol’evich

2017-03-01

Full Text Available The analysis of output and domestic used construction machinery showed that the most vulnerable link remains a problem of underreporting of ergonomics and environmental safety requirements. Compliance with ergonomic requirements enhances the performance of mechanized operations, leading to a decrease in construction costs. Increased environmental requirements reduce the negative impact on air basin. These results suggest the need to improve the structures of building machines. Made recommendations for a comprehensive solution to the problem. The measures to improve environmental safety, ergonomic design requirements of construction machinery, mechanisms of the optimal development of the domestic construction machinery, through the development and implementation of appropriate measures. Keywords: ergonomics of construction equipment, environmental safety of construction equipment

46 CFR 111.103-1 - Power ventilation systems except machinery space ventilation systems.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Power ventilation systems except machinery space ventilation systems. 111.103-1 Section 111.103-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Power ventilation systems except machinery space ventilation systems. Each power ventilation system must...

A Method of Rotating Machinery Fault Diagnosis Based on the Close Degree of Information Entropy

Institute of Scientific and Technical Information of China (English)

GENG Jun-bao; HUANG Shu-hong; JIN Jia-shan; CHEN Fei; LIU Wei

2006-01-01

This paper presents a method of rotating machinery fault diagnosis based on the close degree of information entropy. In the view of the information entropy, we introduce four information entropy features of the rotating machinery, which describe the vibration condition of the machinery. The four features are, respectively, denominated as singular spectrum entropy, power spectrum entropy, wavelet space state feature entropy and wavelet power spectrum entropy. The value scopes of the four information entropy features of the rotating machinery in some typical fault conditions are gained by experiments, which can be acted as the standard features of fault diagnosis. According to the principle of the shorter distance between the more similar models, the decision-making method based on the close degree of information entropy is put forward to deal with the recognition of fault patterns. We demonstrate the effectiveness of this approach in an instance involving the fault pattern recognition of some rotating machinery.

Fault Diagnosis for Rotating Machinery Using Vibration Measurement Deep Statistical Feature Learning

Directory of Open Access Journals (Sweden)

Chuan Li

2016-06-01

Full Text Available Fault diagnosis is important for the maintenance of rotating machinery. The detection of faults and fault patterns is a challenging part of machinery fault diagnosis. To tackle this problem, a model for deep statistical feature learning from vibration measurements of rotating machinery is presented in this paper. Vibration sensor signals collected from rotating mechanical systems are represented in the time, frequency, and time-frequency domains, each of which is then used to produce a statistical feature set. For learning statistical features, real-value Gaussian-Bernoulli restricted Boltzmann machines (GRBMs are stacked to develop a Gaussian-Bernoulli deep Boltzmann machine (GDBM. The suggested approach is applied as a deep statistical feature learning tool for both gearbox and bearing systems. The fault classification performances in experiments using this approach are 95.17% for the gearbox, and 91.75% for the bearing system. The proposed approach is compared to such standard methods as a support vector machine, GRBM and a combination model. In experiments, the best fault classification rate was detected using the proposed model. The results show that deep learning with statistical feature extraction has an essential improvement potential for diagnosing rotating machinery faults.

Fault Diagnosis for Rotating Machinery Using Vibration Measurement Deep Statistical Feature Learning.

Science.gov (United States)

Li, Chuan; Sánchez, René-Vinicio; Zurita, Grover; Cerrada, Mariela; Cabrera, Diego

2016-06-17

Fault diagnosis is important for the maintenance of rotating machinery. The detection of faults and fault patterns is a challenging part of machinery fault diagnosis. To tackle this problem, a model for deep statistical feature learning from vibration measurements of rotating machinery is presented in this paper. Vibration sensor signals collected from rotating mechanical systems are represented in the time, frequency, and time-frequency domains, each of which is then used to produce a statistical feature set. For learning statistical features, real-value Gaussian-Bernoulli restricted Boltzmann machines (GRBMs) are stacked to develop a Gaussian-Bernoulli deep Boltzmann machine (GDBM). The suggested approach is applied as a deep statistical feature learning tool for both gearbox and bearing systems. The fault classification performances in experiments using this approach are 95.17% for the gearbox, and 91.75% for the bearing system. The proposed approach is compared to such standard methods as a support vector machine, GRBM and a combination model. In experiments, the best fault classification rate was detected using the proposed model. The results show that deep learning with statistical feature extraction has an essential improvement potential for diagnosing rotating machinery faults.

Performance of machinery in potato production in one growing season

Directory of Open Access Journals (Sweden)

Kun Zhou

2015-12-01

Full Text Available Statistics on the machinery performance are essential for farm managers to make better decisions. In this paper, the performance of all machineries in five sequential operations, namely bed forming, stone separation, planting, spraying and harvesting in the potato production system, were investigated during one growing season. In order to analyse and decompose the recorded GPS data into various time and distance elements for estimation of the machinery performance, an automatic GPS analysis tool was developed. The field efficiency and field capacity were estimated for each operation. Specifically, the measured average field efficiency was 71.3% for bed forming, 68.5% for stone separation, 40.3% for planting, 69.7% for spraying, and 67.4% for harvesting. The measured average field capacities were 1.46 ha/h, 0.53 ha/h, 0.47 ha/h, 10.21 ha/h, 0.51 ha/h, for the bed forming, stone separation, planting, spraying, and harvesting operations, respectively. These results deviate from the corresponding estimations calculated based on norm data from the American Society of Agricultural and Biological Engineers (ASABE. The deviations indicate that norms provided by ASABE cannot be used directly for the prediction of performance of the machinery used in this work. Moreover, the measured data of bed forming and stone separation could be used as supplementary data for the ASABE which does not provide performance norms for these two operations. The gained results can help farm managers to make better management and operational decisions that result in potential improvement in productivity and profitability as well as in potential environmental benefits.

Performance of machinery in potato production in one growing season

Energy Technology Data Exchange (ETDEWEB)

Zhou, K.; Jensen, A.L.; Bochtis, D.D.; Sørensen, C.G.

2015-07-01

Statistics on the machinery performance are essential for farm managers to make better decisions. In this paper, the performance of all machineries in five sequential operations, namely bed forming, stone separation, planting, spraying and harvesting in the potato production system, were investigated during one growing season. In order to analyse and decompose the recorded GPS data into various time and distance elements for estimation of the machinery performance, an automatic GPS analysis tool was developed. The field efficiency and field capacity were estimated for each operation. Specifically, the measured average field efficiency was 71.3% for bed forming, 68.5% for stone separation, 40.3% for planting, 69.7% for spraying, and 67.4% for harvesting. The measured average field capacities were 1.46 ha/h, 0.53 ha/h, 0.47 ha/h, 10.21 ha/h, 0.51 ha/h, for the bed forming, stone separation, planting, spraying, and harvesting operations, respectively. These results deviate from the corresponding estimations calculated based on norm data from the American Society of Agricultural and Biological Engineers (ASABE). The deviations indicate that norms provided by ASABE cannot be used directly for the prediction of performance of the machinery used in this work. Moreover, the measured data of bed forming and stone separation could be used as supplementary data for the ASABE which does not provide performance norms for these two operations. The gained results can help farm managers to make better management and operational decisions that result in potential improvement in productivity and profitability as well as in potential environmental benefits. (Author)

Machinery and labour force requirements for forest chip production in Finland in 2020

Energy Technology Data Exchange (ETDEWEB)

Kaerhae, K.; Strandsroem, M. (Metsaeteho Oy, Helsinki (Finland)), Email: kalle.karha@metsateho.fi, Email: markus.strandstrom@metsateho.fi; Lahtinen, P.; Elo, J. (Poeyry Energy Oy, Espoo (Finland)), Email: perttu.lahtinen@poyry.com, Email: juha.elo@poyry.com

2009-07-01

The research carried out by Metsaeteho Oy and Poeyry Energy Oy estimated how much machinery and labour would be needed for large-scale forest chip production if the use of forest chips increases extensively in Finland during the coming decade. If the production and consumption of forest chips are 25 to 30 TWh in Finland 2020, then 1,900 to 2,200 machinery units, i.e. machines and trucks, would be needed. This would mean total investments in production machinery of 530 to 630 million euro (VAT 0 %). The labour demand would be 3,400 to 4,000 machine operators and drivers, and 4,200 to 5,100 labour years including indirect labour. Respectively, if the production and consumption of forest chips is 15 to 20 TWh in Finland in 2020, then the production machinery requirement would be 1,100 to 1,500 machines and trucks. The total machinery investment cost would be 320 to 420 million euro (VAT 0 %) and the calculated labour demand 2,000 to 2,700 machine operators and drivers (2,500 to 3,400 labour years). The results of the study indicated that forest chip production resources will be a major bottleneck in reaching the consumption target of 12 million m3, i.e. around 24 TWh of forest chips in Finland by 2020. (orig.)

Spatiotemporal Regulation of Nuclear Transport Machinery and Microtubule Organization

Science.gov (United States)

Okada, Naoyuki; Sato, Masamitsu

2015-01-01

Spindle microtubules capture and segregate chromosomes and, therefore, their assembly is an essential event in mitosis. To carry out their mission, many key players for microtubule formation need to be strictly orchestrated. Particularly, proteins that assemble the spindle need to be translocated at appropriate sites during mitosis. A small GTPase (hydrolase enzyme of guanosine triphosphate), Ran, controls this translocation. Ran plays many roles in many cellular events: nucleocytoplasmic shuttling through the nuclear envelope, assembly of the mitotic spindle, and reorganization of the nuclear envelope at the mitotic exit. Although these events are seemingly distinct, recent studies demonstrate that the mechanisms underlying these phenomena are substantially the same as explained by molecular interplay of the master regulator Ran, the transport factor importin, and its cargo proteins. Our review focuses on how the transport machinery regulates mitotic progression of cells. We summarize translocation mechanisms governed by Ran and its regulatory proteins, and particularly focus on Ran-GTP targets in fission yeast that promote spindle formation. We also discuss the coordination of the spatial and temporal regulation of proteins from the viewpoint of transport machinery. We propose that the transport machinery is an essential key that couples the spatial and temporal events in cells. PMID:26308057

Research on bearing fault diagnosis of large machinery based on mathematical morphology

Science.gov (United States)

Wang, Yu

2018-04-01

To study the automatic diagnosis of large machinery fault based on support vector machine, combining the four common faults of the large machinery, the support vector machine is used to classify and identify the fault. The extracted feature vectors are entered. The feature vector is trained and identified by multi - classification method. The optimal parameters of the support vector machine are searched by trial and error method and cross validation method. Then, the support vector machine is compared with BP neural network. The results show that the support vector machines are short in time and high in classification accuracy. It is more suitable for the research of fault diagnosis in large machinery. Therefore, it can be concluded that the training speed of support vector machines (SVM) is fast and the performance is good.

Application of Choi—Williams Reduced Interference Time Frequency Distribution to Machinery Diagnostics

Directory of Open Access Journals (Sweden)

Howard A. Gaberson

1995-01-01

Full Text Available This article discusses time frequency analysis of machinery diagnostic vibration signals. The short time Fourier transform, the Wigner, and the Choi–Williams distributions are explained and illustrated with test cases. Examples of Choi—Williams analyses of machinery vibration signals are presented. The analyses detect discontinuities in the signals and their timing, amplitude and frequency modulation, and the presence of different components in a vibration signal.

An Association of Unique microRNA Turnover Machinery with Prostate Cancer Progression

Science.gov (United States)

2017-10-01

targeting of critical androgen receptor -604 coregulator interactions in prostate cancer . Nature communications 4, 1923, 605 doi:10.1038/ncomms2912 (2013...AWARD NUMBER: W81XWH-16-1-0474 TITLE: An Association of Unique microRNA Turnover Machinery with Prostate Cancer Progression PRINCIPAL INVESTIGATOR...14 Sep 2017 4. Title An Association of Unique microRNA Turnover Machinery with Prostate Cancer Progression 5a. CONTRACT NUMBER 5b. GRANT NUMBER

Molecular building blocks and their architecture in biologically/environmentally compatible soft matter chemical machinery.

Science.gov (United States)

Toyota, Taro; Banno, Taisuke; Nitta, Sachiko; Takinoue, Masahiro; Nomoto, Tomonori; Natsume, Yuno; Matsumura, Shuichi; Fujinami, Masanori

2014-01-01

This review briefly summarizes recent developments in the construction of biologically/environmentally compatible chemical machinery composed of soft matter. Since environmental and living systems are open systems, chemical machinery must continuously fulfill its functions not only through the influx and generation of molecules but also via the degradation and dissipation of molecules. If the degradation or dissipation of soft matter molecular building blocks and biomaterial molecules/polymers can be achieved, soft matter particles composed of them can be used to realize chemical machinery such as selfpropelled droplets, drug delivery carriers, tissue regeneration scaffolds, protocell models, cell-/tissuemarkers, and molecular computing systems.

The RNAi Inheritance Machinery of Caenorhabditis elegans.

Science.gov (United States)

Spracklin, George; Fields, Brandon; Wan, Gang; Becker, Diveena; Wallig, Ashley; Shukla, Aditi; Kennedy, Scott

2017-07-01

Gene silencing mediated by dsRNA (RNAi) can persist for multiple generations in Caenorhabditis elegans (termed RNAi inheritance). Here we describe the results of a forward genetic screen in C. elegans that has identified six factors required for RNAi inheritance: GLH-1/VASA, PUP-1/CDE-1, MORC-1, SET-32, and two novel nematode-specific factors that we term here (heritable RNAi defective) HRDE-2 and HRDE-4 The new RNAi inheritance factors exhibit mortal germline (Mrt) phenotypes, which we show is likely caused by epigenetic deregulation in germ cells. We also show that HRDE-2 contributes to RNAi inheritance by facilitating the binding of small RNAs to the inheritance Argonaute (Ago) HRDE-1 Together, our results identify additional components of the RNAi inheritance machinery whose conservation provides insights into the molecular mechanism of RNAi inheritance, further our understanding of how the RNAi inheritance machinery promotes germline immortality, and show that HRDE-2 couples the inheritance Ago HRDE-1 with the small RNAs it needs to direct RNAi inheritance and germline immortality. Copyright © 2017 by the Genetics Society of America.

Machinery health prognostics: A systematic review from data acquisition to RUL prediction

Science.gov (United States)

Lei, Yaguo; Li, Naipeng; Guo, Liang; Li, Ningbo; Yan, Tao; Lin, Jing

2018-05-01

Machinery prognostics is one of the major tasks in condition based maintenance (CBM), which aims to predict the remaining useful life (RUL) of machinery based on condition information. A machinery prognostic program generally consists of four technical processes, i.e., data acquisition, health indicator (HI) construction, health stage (HS) division, and RUL prediction. Over recent years, a significant amount of research work has been undertaken in each of the four processes. And much literature has made an excellent overview on the last process, i.e., RUL prediction. However, there has not been a systematic review that covers the four technical processes comprehensively. To fill this gap, this paper provides a review on machinery prognostics following its whole program, i.e., from data acquisition to RUL prediction. First, in data acquisition, several prognostic datasets widely used in academic literature are introduced systematically. Then, commonly used HI construction approaches and metrics are discussed. After that, the HS division process is summarized by introducing its major tasks and existing approaches. Afterwards, the advancements of RUL prediction are reviewed including the popular approaches and metrics. Finally, the paper provides discussions on current situation, upcoming challenges as well as possible future trends for researchers in this field.

Availability analysis of selected mining machinery

Directory of Open Access Journals (Sweden)

Brodny Jarosław

2017-06-01

Full Text Available Underground extraction of coal is characterized by high variability of mining and geological conditions in which it is conducted. Despite ever more effective methods and tools, used to identify the factors influencing this process, mining machinery, used in mining underground, work in difficult and not always foreseeable conditions, which means that these machines should be very universal and reliable. Additionally, a big competition, occurring on the coal market, causes that it is necessary to take action in order to reduce the cost of its production, e.g. by increasing the efficiency of utilization machines. To meet this objective it should be pro-ceed with analysis presented in this paper. The analysis concerns to availability of utilization selected mining machinery, conducted using the model of OEE, which is a tool for quantitative estimate strategy TPM. In this article we considered the machines being part of the mechanized longwall complex and the basis of analysis was the data recording by the industrial automation system. Using this data set we evaluated the availability of studied machines and the structure of registered breaks in their work. The results should be an important source of information for maintenance staff and management of mining plants, needed to improve the economic efficiency of underground mining.

Comparison of Management-Operational Efficiency of Agricultural Machinery Operating Systems (Case Study Alborz Province

Directory of Open Access Journals (Sweden)

A Omidi

2017-10-01

Full Text Available Introduction Measuring the efficiency of operating systems in comparison with the methods of comparing the performance of systems explains the various dimensions of issues such as, the lack of full use of agricultural machinery capacity, improper selection of machine, incorrect use of machinery, ownership, etc.. Any improvement in operating system conditions reduces costs,, consumption of inputs, increases the efficiency of production factors and consequently reduces the price and increases agricultural profitability. The main objective of this research is to compare the operational-management efficiency of operating systems in Alborz province and comparison of managerial and operational efficiency of agricultural machinery farming systems by calculating the efficiency of its major components in agricultural machinery farming systems including efficiency, social, economic, technical-operational and managerial and ranking them in order to understand the optimal model of agricultural machinery systems. Materials and Methods This research is a survey study.The study population was beneficiaries of agricultural machinery in the Alborz province which in the multi-stage random sample was determined. Alborz province has 31,438 agricultural operations, of which 543 are exploited agricultural machinery. Cochran formula was used to determine sample size. Since, Cronbach's alpha coefficient greater than 0.7 was obtained by questionnaire, the reliability of the questionnaires was assessed as desirable. To calculate the efficiency the component data were extracted from 4 specialized questionnaires after the initial examination and encoding, then they were analyzed using the software SPSS, MCDM Engine. TOPSIS techniques were used for ranking managerial performance operating system for operating agricultural machinery Alborz province. Results and Discussion The results showed that social efficiency of dedicated-professional operation with an average of 6.6 had

[Effects of heavy machinery operation on the structural characters of cultivated soils in black soil region of Northeast China].

Science.gov (United States)

Wang, En-Heng; Chai, Ya-Fan; Chen, Xiang-Wei

2008-02-01

With the cultivated soils in black soil region of Northeast China as test objects, this paper measured their structural characters such as soil strength, bulk density, and non-capillary porosity/capillary porosity (NCP/CP) ratio before and after heavy and medium-sized machinery operation, aimed to study the effects of machinery operation on the physical properties of test soils. The results showed that after machinery operation, there existed three distinct layers from top to bottom in the soil profiles, i.e., plowed layer, cumulative compacted layer, and non-affected layer, according to the changes of soil strength. Under medium-sized machinery operation, these three layers were shallower, and there was a new plow pan at the depth between 17.5 and 30 cm. Heavy machinery operation had significant positive effects on the improvement of topsoil structure (P heavy machinery, the bulk density of topsoil decreased by 7.2% and 3.5%, respectively, and NCP/CP increased by 556.6% after subsoiling, which would benefit water infiltration, reinforce water storage, and weaken the threat of soil erosion. The main action of heavy machinery operation was soil loosening, while that of medium-sized machinery operation was soil compacting.

On the reversed Brayton cycle with high speed machinery

Energy Technology Data Exchange (ETDEWEB)

Backman, J.

1996-12-31

This work was carried out in the laboratory of Fluid Dynamics, at Lappeenranta University of Technology during the years 1991-1996. The research was a part of larger high speed technology development research. First, there was the idea of making high speed machinery applications with the Brayton cycle. There was a clear need to deepen the knowledge of the cycle itself and to make a new approach in the field of the research. Also, the removal of water from the humid air seemed very interesting. The goal of this work was to study methods of designing high speed machinery for the reversed Brayton cycle, from theoretical principles to practical applications. The reversed Brayton cycle can be employed as an air dryer, a heat pump or a refrigerating machine. In this research the use of humid air as a working fluid has an environmental advantage, as well. A new calculation method for the Brayton cycle is developed. In this method especially the expansion process in the turbine is important because of the condensation of the water vapour in the humid air. This physical phenomena can have significant effects on the level of performance of the application. Also, the influence of calculating the process with actual, achievable process equipment efficiencies is essential for the development of future machinery. The above theoretical calculations are confirmed with two different laboratory prototypes. (53 refs.)

On the reversed Brayton cycle with high speed machinery

Energy Technology Data Exchange (ETDEWEB)

Backman, J

1997-12-31

This work was carried out in the laboratory of Fluid Dynamics, at Lappeenranta University of Technology during the years 1991-1996. The research was a part of larger high speed technology development research. First, there was the idea of making high speed machinery applications with the Brayton cycle. There was a clear need to deepen the knowledge of the cycle itself and to make a new approach in the field of the research. Also, the removal of water from the humid air seemed very interesting. The goal of this work was to study methods of designing high speed machinery for the reversed Brayton cycle, from theoretical principles to practical applications. The reversed Brayton cycle can be employed as an air dryer, a heat pump or a refrigerating machine. In this research the use of humid air as a working fluid has an environmental advantage, as well. A new calculation method for the Brayton cycle is developed. In this method especially the expansion process in the turbine is important because of the condensation of the water vapour in the humid air. This physical phenomena can have significant effects on the level of performance of the application. Also, the influence of calculating the process with actual, achievable process equipment efficiencies is essential for the development of future machinery. The above theoretical calculations are confirmed with two different laboratory prototypes. (53 refs.)

Machinery fault diagnosis using joint global and local/nonlocal discriminant analysis with selective ensemble learning

Science.gov (United States)

Yu, Jianbo

2016-11-01

The vibration signals of faulty machine are generally non-stationary and nonlinear under those complicated working conditions. Thus, it is a big challenge to extract and select the effective features from vibration signals for machinery fault diagnosis. This paper proposes a new manifold learning algorithm, joint global and local/nonlocal discriminant analysis (GLNDA), which aims to extract effective intrinsic geometrical information from the given vibration data. Comparisons with other regular methods, principal component analysis (PCA), local preserving projection (LPP), linear discriminant analysis (LDA) and local LDA (LLDA), illustrate the superiority of GLNDA in machinery fault diagnosis. Based on the extracted information by GLNDA, a GLNDA-based Fisher discriminant rule (FDR) is put forward and applied to machinery fault diagnosis without additional recognizer construction procedure. By importing Bagging into GLNDA score-based feature selection and FDR, a novel manifold ensemble method (selective GLNDA ensemble, SE-GLNDA) is investigated for machinery fault diagnosis. The motivation for developing ensemble of manifold learning components is that it can achieve higher accuracy and applicability than single component in machinery fault diagnosis. The effectiveness of the SE-GLNDA-based fault diagnosis method has been verified by experimental results from bearing full life testers.

The phototransduction machinery in the rod outer segment has a strong efficacy gradient

KAUST Repository

Mazzolini, Monica

2015-05-04

Rod photoreceptors consist of an outer segment (OS) and an inner segment. Inside the OS a biochemical machinery transforms the rhodopsin photoisomerization into electrical signal. This machinery has been treated as and is thought to be homogenous with marginal inhomogeneities. To verify this assumption, we developed a methodology based on special tapered optical fibers (TOFs) to deliver highly localized light stimulations. By using these TOFs, specific regions of the rod OS could be stimulated with spots of light highly confined in space. As the TOF is moved from the OS base toward its tip, the amplitude of saturating and single photon responses decreases, demonstrating that the efficacy of the transduction machinery is not uniform and is 5-10 times higher at the base than at the tip. This gradient of efficacy of the transduction machinery is attributed to a progressive depletion of the phosphodiesterase along the rod OS. Moreover we demonstrate that, using restricted spots of light, the duration of the photoresponse along the OS does not increase linearly with the light intensity as with diffuse light. © 2015, National Academy of Sciences. All rights reserved.

Non-machinery dialysis that achieves blood purification therapy without using full-scale dialysis machines.

Science.gov (United States)

Abe, Takaya; Onoda, Mistutaka; Matsuura, Tomohiko; Sugimura, Jun; Obara, Wataru; Sato, Toshiya; Takahashi, Mihoko; Chiba, Kenta; Abe, Tomiya

2017-09-01

An electrical or water supply and a blood purification machine are required for renal replacement therapy. There is a possibility that acute kidney injury can occur in large numbers and on a wide scale in the case of a massive earthquake, and there is the potential risk that the current supply will be unable to cope with acute kidney injury cases. However, non-machinery dialysis requires exclusive circuits and has the characteristic of not requiring the full-scale dialysis machines. We performed perfusion experiments that used non-machinery dialysis and recent blood purification machines in 30-min intervals, and the effectiveness of non-machinery dialysis was evaluated by the assessing the removal efficiency of potassium, which causes lethal arrhythmia during acute kidney injury. The non-machinery dialysis potassium removal rate was at the same level as continuous blood purification machines with a dialysate flow rate of 5 L/h after 15 min and continuous blood purification machines with a dialysate flow rate of 3 L/h after 30 min. Non-machinery dialysis required an exclusive dialysate circuit, the frequent need to replace bags, and new dialysate exchanged once every 30 min. However, it can be seen as an effective renal replacement therapy for crush-related acute kidney injury patients, even in locations or facilities not having the full-scale dialysis machines.

Acoustic Camera as a Fool for Identifying Machinery and Equipment Failures

Directory of Open Access Journals (Sweden)

Ľudmila Pavlikova

2018-03-01

Full Text Available Sound and noise are as old as humanity itself. They have accompanied civilization, evolution, and development for centuries. Music and speech represent not only the key elements of human life but also unpleasant feelings of noise that have always been an integral part of human existence. As industrial development has required more energy, powerful machinery, and equipment, there have been still noisier machines. Traffic has grown quickly due to the number and speed of vehicles. For that reason, an acoustic camera is used for the dynamic visualization of machinery and equipment noise as it analyses the sources of noise in details. Subsequently, qualified measures are introduced based on the results of the analysis. The paper considers launching another application. According to the proposed methodology, its use in identifying machinery and equipment failures and their maintenance is proved. The experiment was performed on a four-wheel lawn mower. The primary focus was on the identification of failures using an acoustic camera. The previous method allowed to quickly, precisely and efficiently identifying the failures in two out of five tested machines.

Fluorescence excitation-emission matrix spectroscopy for degradation monitoring of machinery lubricants

Science.gov (United States)

Sosnovski, Oleg; Suresh, Pooja; Dudelzak, Alexander E.; Green, Benjamin

2018-02-01

Lubrication oil is a vital component of heavy rotating machinery defining the machine's health, operational safety and effectiveness. Recently, the focus has been on developing sensors that provide real-time/online monitoring of oil condition/lubricity. Industrial practices and standards for assessing oil condition involve various analytical methods. Most these techniques are unsuitable for online applications. The paper presents the results of studying degradation of antioxidant additives in machinery lubricants using Fluorescence Excitation-Emission Matrix (EEM) Spectroscopy and Machine Learning techniques. EEM Spectroscopy is capable of rapid and even standoff sensing; it is potentially applicable to real-time online monitoring.

Safeness of radiological machinery

International Nuclear Information System (INIS)

Yokoyama, Shun

1979-01-01

The human factors affecting the safeness of radiological machinery, which are often very big and complicated machines, are described from the stand point of handling. 20 to 50% of the troubles on equipments seem to be caused by men. This percentage will become even higher in highly developed equipments. Human factors have a great influence on the safeness of radiological equipments. As the human factors, there are sensory factors and knowledge factors as well as psychological factors, and the combination of these factors causes mishandling and danger. Medical services at present are divided in various areas, and consist of the teamwork of the people in various professions. Good human relationship, education and control are highly required to secure the safeness. (Kobatake, H.)

Welding technologies for nuclear machinery and equipment

International Nuclear Information System (INIS)

Kobayashi, Masahiro; Yokono, Tomomi.

1991-01-01

The main welding methods applied to nuclear machinery and equipment are shielded metal arc welding, submerged arc welding, MAG welding and TIG welding. But in the last 10 years, in order to improve the reliability required for the welding of nuclear machinery and equipment, the welding technologies aiming at the reduction of heat input, the decrease of the number of welding pass and the automatic control of welding factors have been applied for the main purpose of bettering the quality and excluding human errors. The merits and the technology of narrow gap, pulsed MAG welding and melt-through welding are explained. As the automation of TIG welding, image processing type narrow gap, hot wire TIG welding and remote control type automatic TIG welding are described. For the longitudinal welding of active metal sheet products, plasma key-hole welding is applied. Since the concentration of its arc is good, high speed welding with low heat input can be done. For the stainless steel cladding by welding, electroslag welding has become to be employed in place of conventional submerged arc welding. Arc is not generated in the electroslag welding, and the penetration into base metal is small. (K.I.)

Rotating Machinery Predictive Maintenance Through Expert System

Directory of Open Access Journals (Sweden)

M. Sarath Kumar

2000-01-01

Full Text Available Modern rotating machines such as turbomachines, either produce or absorb huge amount of power. Some of the common applications are: steam turbine-generator and gas turbine-compressor-generator trains produce power and machines, such as pumps, centrifugal compressors, motors, generators, machine tool spindles, etc., are being used in industrial applications. Condition-based maintenance of rotating machinery is a common practice where the machine's condition is monitored constantly, so that timely maintenance can be done. Since modern machines are complex and the amount of data to be interpreted is huge, we need precise and fast methods in order to arrive at the best recommendations to prevent catastrophic failure and to prolong the life of the equipment. In the present work using vibration characteristics of a rotor-bearing system, the condition of a rotating machinery (electrical rotor is predicted using an off-line expert system. The analysis of the problem is carried out in an Object Oriented Programming (OOP framework using the finite element method. The expert system which is also developed in an OOP paradigm gives the type of the malfunctions, suggestions and recommendations. The system is implemented in C++.

46 CFR 35.25-1 - Examination of boilers and machinery by engineer-T/ALL.

Science.gov (United States)

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Examination of boilers and machinery by engineer-T/ALL... Engine Department § 35.25-1 Examination of boilers and machinery by engineer—T/ALL. It shall be the duty of an engineer when assuming charge of the boilers to examine the same forthwith and thoroughly. If...

Machinery prognostics and prognosis oriented maintenance management

CERN Document Server

Yan, Jihong

2014-01-01

This book gives a complete presentatin of the basic essentials of machinery prognostics and prognosis oriented maintenance management, and takes a look at the cutting-edge discipline of intelligent failure prognosis technologies for condition-based maintenance.  Latest research results and application methods are introduced for signal processing, reliability moelling, deterioration evaluation, residual life prediction and maintenance-optimization as well as applications of these methods.

Optimization Model for Machinery Selection of Multi-Crop Farms in Elsuki Agricultural Scheme

Directory of Open Access Journals (Sweden)

Mysara Ahmed Mohamed

2017-07-01

Full Text Available The optimization machinery model was developed to aid decision-makers and farm machinery managers in determining the optimal number of tractors, scheduling the agricultural operation and minimizing machinery total costs. For purpose of model verification, validation and application input data was collected from primary & secondary sources from Elsuki agricultural scheme for two seasons namely 2011-2012 and 2013-2014. Model verification was made by comparing the numbers of tractors of Elsuki agricultural scheme for season 2011-2012 with those estimated by the model. The model succeeded in reducing the number of tractors and operation total cost by 23%. The effect of optimization model on elements of direct cost saving indicated that the highest cost saving is reached with depreciation, repair and maintenance (23% and the minimum cost saving is attained with fuel cost (22%. Sensitivity analysis in terms of change in model input for each of cultivated area and total costs of operations showing that: Increasing the operation total cost by 10% decreased the total number of tractors after optimization by 23% and total cost of operations was also decreased by 23%. Increasing the cultivated area by 10%, decreased the total number of tractors after optimization by(12% and total cost of operations was also decreased by 12% (16669206 SDG(1111280 $ to 14636376 SDG(975758 $. For the case of multiple input effect of the area and operation total cost resulted in decrease maximum number of tractors by 12%, and the total cost of operations also decreased by 12%. It is recommended to apply the optimization model as pre-requisite for improving machinery management during implementation of machinery scheduling.

Machinery for Forest Chip Production in Finland in 2007 and in the Future

Energy Technology Data Exchange (ETDEWEB)

Kaerhae, Kalle (Metsaeteho Oy, P.O. Box 101, FI-00171 Helsinki (Finland))

2008-10-15

Metsaeteho Oy's study consisted of a survey of the production machinery for forest chips used by energy plants in 2007. The major forest chip suppliers in Finland were involved in the study. In addition, the machinery and equipment stocked by the manufacturers and vendors of energy wood harvester heads, stump lifting devices, and chippers were also surveyed. The study provided also an estimate of future machinery requirements for forest chip production in Finland. The study estimated that a total of 1,100 machine and truck units were employed in the production of forest chips for energy plants in 2007. A total of 770 machine and truck units were contracted for the major forest chip suppliers in 2007. Increasing forest chip consumption will considerable increase the demand for additional forest chip production resources in the future. If the consumption of forest chips by energy plants in 2015 reaches 15 TWh, i.e. about 7.5 mill. m3, then the forest machine and truck requirement will be over 1,700 units. The corresponding machinery requirement at an energy plant with a forest chip consumption of 25 TWh (approx. 12.5 mill. m3), will be close to 2,300 machine and truck units

Power electronics and electric machinery challenges and opportunities in electric and hybrid vehicles

Energy Technology Data Exchange (ETDEWEB)

Adams, D.J.; Hsu, J.S.; Young, R.W. [Oak Ridge National Lab., TN (United States); Peng, F.Z. [Univ. of Tennessee, Knoxville, TN (United States)

1997-06-01

The development of power electronics and electric machinery presents significant challenges to the advancement of electric and hybrid vehicles. Electronic components and systems development for vehicle applications have progressed from the replacement of mechanical systems to the availability of features that can only be realized through interacting electronic controls and devices. Near-term applications of power electronics in vehicles will enable integrated powertrain controls, integrated chassis system controls, and navigation and communications systems. Future applications of optimized electric machinery will enable highly efficient and lightweight systems. This paper will explore the areas where research and development is required to ensure the continued development of power electronics and electric machines to meet the rigorous demands of automotive applications. Additionally, recent advances in automotive related power electronics and electric machinery at Oak Ridge National Laboratory will be explained. 3 refs., 5 figs.

The experimental study on efficiency improvement of turbo machinery supported with magnetic bearings

International Nuclear Information System (INIS)

Park, In Hwang; Park, Young Ho; Han, Dong Chul

2007-01-01

To implement a conventional electromagnetic bearing in small turbo machinery, it has problems such as load capacity and size. Therefore, in this paper, these problems are resolved by using a permanent magnet biased electromagnetic bearing as a thrust bearing of small turbo machinery. Because the flux path of the bearing is designed by reluctance path modulation using an electromagnet and a permanent magnet, the bearing improves upon non-linearity, power consumption, size and load capacity of a conventional electromagnetic bearing. Test rotating the shaft over 500,000DN were carried out to verify the performance of the proposed small turbo machinery. In addition, the relationships between mass flow rate and pressure rise were measured as changing the tip clearance to verify the feasibility of efficiency improvement and active surge control and these results were compared with theoretical results

Evaluation of radiofrequency dielectric heaters workers exposure

International Nuclear Information System (INIS)

Benes, M.; Del Frate, S.; Villalta, R.

2008-01-01

Radiofrequency dielectric heaters (RFDH) are widely used in the woodworking industry for gluing laminates by applying pressure and RF heating. The workers operating such equipment remain in the vicinity of the machinery all day and can therefore be exposed to considerable levels of electric and magnetic field at RFs. This work describes the method used to measure the strength of fields generated by this particular machinery. This procedure is based on current methods cited in the literature and introduces the necessary modifications to meet this specific case. In particular, as there is often a scarcity of technical data available relating to such heaters, it is suggested that a spectrum analyser be used for measurements in the frequencies domain. On the basis of the data obtained the norms of reference are established, the instrumentation to be used in successive stages determined as well as the identification of possible sources of interference from spurious signals. Furthermore, a mapping of the field strengths is presented and the means of determining the decay curve as a function of distance. This last type of measurement is done to estimate the effectiveness of grounding the machinery. The report ends with an estimate of the exposure of workers to electromagnetic fields and also some recommendations for reducing risk. (authors)

Fluid machinery application, selection, and design

CERN Document Server

Wright, Terry

2013-01-01

Published nearly a decade ago, Fluid Machinery: Performance, Analysis, and Design quickly became popular with students, professors, and professionals because of its comprehensive and comprehensible introduction to the fluid mechanics of turbomachinery. Renamed to reflect its wider scope and reorganized content, this second edition provides a more logical flow of information that will enhance understanding. In particular, it presents a consistent notation within and across chapters, updating material when appropriate. Although the authors do account for the astounding growth in the field of com

The kinematics of machinery outlines of a theory of machines

CERN Document Server

Reuleaux, Franz

2012-01-01

A classic on the kinematics of machinery, this volume was written by the Father of Kinematics. Reuleaux writes with authority and precision, developing the subject from its fundamentals. 450 figures. 1876 edition.

The Design of High Reliability Magnetic Bearing Systems for Helium Cooled Reactor Machinery

International Nuclear Information System (INIS)

Swann, M.; Davies, N.; Jayawant, R.; Leung, R.; Shultz, R.; Gao, R.; Guo, Z.

2014-01-01

The requirements for magnetic bearing equipped machinery used in high temperature, helium cooled, graphite moderated reactor applications present a set of design considerations that are unlike most other applications of magnetic bearing technology in large industrial rotating equipment, for example as used in the oil and gas or other power generation applications. In particular, the bearings are typically immersed directly in the process gas in order to take advantage of the design simplicity that comes about from the elimination of ancillary lubrication and cooling systems for bearings and seals. Such duty means that the bearings will usually see high temperatures and pressures in service and will also typically be subject to graphite particulate and attendant radioactive contamination over time. In addition, unlike most industrial applications, seismic loading events become of paramount importance for the magnetic bearings system, both for actuators and controls. The auxiliary bearing design requirements, in particular, become especially demanding when one considers that the whole mechanical structure of the magnetic bearing system is located inside an inaccessible pressure vessel that should be rarely, if ever, disassembled over the service life of the power plant. Lastly, many machinery designs for gas cooled nuclear power plants utilize vertical orientation. This circumstance presents its own unique requirements for the machinery dynamics and bearing loads. Based on the authors’ experience with machine design and supply on several helium cooled reactor projects including Ft. St. Vrain (US), GT-MHR (Russia), PBMR (South Africa), GTHTR (Japan), and most recently HTR-PM (China), this paper addresses many of the design considerations for such machinery and how the application of magnetic bearings directly affects machinery reliability and availability, operability, and maintainability. Remote inspection and diagnostics are a key focus of this paper. (author)

Managing the exploitation life of the mining machinery for an unlimited duration of time

Directory of Open Access Journals (Sweden)

Vujić Slobodan

2011-01-01

Full Text Available The problem of determining the optimum exploitation life of machinery, namely, the optimum time for machinery and equipment replacement, represents a complex and highly responsible engineering task. Taking into consideration the situation prevailing at coal pit mines in Serbia, the tasks of this rank are very complex and difficult. To make a decision on the replacement of capital equipment and machinery, such as bucket wheel excavators within the mentioned systems, implies a management task of utmost responsibility. It requires high professional and analytical knowledge as well as reliable arguments, based on a multidisciplinary professional approach. In this paper, the authors present their views on the problem of establishing the optimum exploitation life of bucket wheel excavators, offering an algorithm, based on dynamic programming, as a solution.

Noise test system of rotating machinery in nuclear power station based on microphone array

Science.gov (United States)

Chang, Xincai; Guan, Jishi; Qi, Liangcai

2017-12-01

Rotating machinery plays an important role in all walks of life. Once the equipment fails, equipment maintenance and shutdown will cause great social harm and economic losses. Equipment safety operations at nuclear power stations have always been of top priority. It is prone to noise when the equipment is out of order or aging. Failure to find or develop equipment at the initial stage of equipment failure or ageing will pose a serious threat to the safety of the plant’s equipment. In this paper, sound imaging diagnosis technology is applied as a supplementary method to the condition monitoring and diagnosis system of rotating machinery in nuclear power stations. It provides a powerful guarantee for the condition monitoring and fault diagnosis of rotating machinery in nuclear power stations.

Present Situation and Activities of ISO 14000 series in Heavy Machinery Industries; Jukikai meka no taiou jokyo

Energy Technology Data Exchange (ETDEWEB)

Tomisawa, Yukio. [Ishikawajima-Harima Heavy Industries Corp., Tokyo (Japan). Research Institute

1999-01-15

This paper shows the outline of the present situation and activities of ISO14000 series, environmental management system in heavy machinery industries. Today, main heavy machinery industries have already certified to ISO 14001 until this summer. The aim of the introduction of ISO 14001 are to establish the system improvement continuously by PDCA, to have essential elements in international trade and to keep up with the risk management. Some example of conducted ISO 14001 in heavy machinery industries are shown. The trend of heavy machinery industries will be able to predict to three key words, internationalization, network and opening information. The new role expected to environmental material engineer are indicated to research and develop ecomaterial by using LCA and to support the recycling, waste treatment and other technical basis of the environmental management system. (author)

A Modelling Approach to Multibody Dynamics of Fluid Power Machinery with Hydrodynamic Lubrication

DEFF Research Database (Denmark)

Johansen, Per; Rømer, Daniel; Andersen, Torben Ole

2013-01-01

The efficiency potential of the digital displacement technology and the increasing interest in hydraulic transmissions in wind and wave energy applications has created an incentive for development of high efficiency fluid power machinery. Modelling and analysis of fluid power machinery loss...... mechanisms is necessary in order to accommodate this demand. At present fully coupled thermo-elastic models for various tribological interfaces has been presented. However, in order to analyse the interaction between tribological interfaces in fluid power pumps and motors, these interface models needs...

Production method of hydrogen jet plasma process in hydro machinery

International Nuclear Information System (INIS)

Amini, F.

2007-01-01

The purpose of present paper is to the process of plasma formation in hydro machinery when a hydro turbine operates at various conditions and load rejection. By investigation the power, shock pressure , and impact effects of hydro machinery, it is revealed that energy and hydrogen are generated by the plasma process. The investigation on several turbines of various hydro power plants reveals that cold fusion process in hydro machinery generates hydrogen. The hypothesis concerning the participation of alkaline metals in river water and the atomic nuclei of the runner blade material in the formation of hydrogen are considered. It is possible to assume hydrogen, deuterium, helium, and tritium atoms (based on Dr. Mizuno and Dr. Kanarev theories) that are formed, diffuse into cavitation bubbles. The plasma is generated during the collapse of the bubble; thus, the quantity of burnt hydrogen determine the volume of generating hydrogen and the impact force caused by hydrogen explosion (noise).There are five main notions, which can determine hydrogen and plasma process: (1) turbine power effect, (2) high shock pressure, (3) crack on turbine parts, (4) impacts effects and (4) the lift of rotating parts. The frequency of the excitation lies in a range from 0.786 to 1.095 Hz.In future, it may be possible to design hydro turbines based on the plasma process that generates hydrogen; or there may exist turbines that rotate with a mixture of hydrogen explosion and water energies

Rotating machinery surveillance system reduces plant downtime and radiation exposure

International Nuclear Information System (INIS)

Bohanick, J.S.; Robinson, J.C.; Allen, J.W.

1988-01-01

A rotating machinery surveillance system (RMSS) was permanently installed at Grand Gulf nuclear station (GGNS) as part of a program sponsored by the US Department of Energy whose goal was to reduce radiation exposure to power plant personnel resulting from the inspection, maintenance, and repair of rotating machinery. The RMSS was installed at GGNS in 1983 to continuously monitor 173 analog vibration signals from proximity probes mounted on 26 machine trains and ∼450 process data points via a computer data link. Vibration frequency spectra, i.e., the vibration amplitude versus frequency of vibration, and various characterizations of these spectra are the fundamental data collected by the RMSS for performing machinery diagnostics. The RMSS collects vibration frequency spectra on a daily basis for all the monitored rotating equipment and automatically stores the collected spectra for review by the vibration engineer. Vibration spectra automatically stored by the RMSS fall into categories that include the last normal, alarm, minimum and maximum, past three-day data set, baseline, current, and user-saved spectra. During first and second fuel-cycle operation at GGNS, several significant vibration problems were detected by the RMSS. Two of these are presented in this paper: recirculation pumps and turbine-generator bearing degradation. The total reduction in personnel radiation exposure at GGNS from 1985 to 1987 due to the presence of the RMSS was estimated to be in the range from 49 to 54 person-rem

46 CFR 182.460 - Ventilation of spaces containing machinery powered by, or fuel tanks for, gasoline.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Ventilation of spaces containing machinery powered by, or fuel tanks for, gasoline. 182.460 Section 182.460 Shipping COAST GUARD, DEPARTMENT OF HOMELAND..., gasoline. (a) A space containing machinery powered by, or fuel tanks for, gasoline must have a ventilation...

Multiple Cylinder Free-Piston Stirling Machinery

Science.gov (United States)

Berchowitz, David M.; Kwon, Yong-Rak

In order to improve the specific power of piston-cylinder type machinery, there is a point in capacity or power where an advantage accrues with increasing number of piston-cylinder assemblies. In the case of Stirling machinery where primary energy is transferred across the casing wall of the machine, this consideration is even more important. This is due primarily to the difference in scaling of basic power and the required heat transfer. Heat transfer is found to be progressively limited as the size of the machine increases. Multiple cylinder machines tend to preserve the surface area to volume ratio at more favorable levels. In addition, the spring effect of the working gas in the so-called alpha configuration is often sufficient to provide a high frequency resonance point that improves the specific power. There are a number of possible multiple cylinder configurations. The simplest is an opposed pair of piston-displacer machines (beta configuration). A three-cylinder machine requires stepped pistons to obtain proper volume phase relationships. Four to six cylinder configurations are also possible. A small demonstrator inline four cylinder alpha machine has been built to demonstrate both cooling operation and power generation. Data from this machine verifies theoretical expectations and is used to extrapolate the performance of future machines. Vibration levels are discussed and it is argued that some multiple cylinder machines have no linear component to the casing vibration but may have a nutating couple. Example applications are discussed ranging from general purpose coolers, computer cooling, exhaust heat power extraction and some high power engines.

INTEGRAL ASSESSMENT OF EFFICIENCY OF A FLEET OF AGRICULTURAL MACHINERY AND TRACTORS

Directory of Open Access Journals (Sweden)

V. M. Korotchenya

2015-01-01

Full Text Available An indicator for an integral assessment of efficiency of a fleet of agricultural machinery and tractors is proposed. Its calculating consists in multiplying partial efficiencies together: technical and price efficiency of agricultural production, and eco-efficiency of a fleet of agricultural machinery and tractors. Using an axiomatic method, the concept of efficiency of fleet was studied within broader category of productivity and in wider scales - within agriculture. The most general and obvious statements according to which efficiency of machine and tractor fleet is considered as indirectly settlement size counted proceeding from efficiency of agricultural production, were accepted as axioms. In general, efficiency is a ratio between productivity of an object in question and that of the theoretical ideal or an object with maximum productivity efficiency of which is accepted equal to unity or 100%. Technical efficiency of agricultural production characterizes the ability of an agricultural sector to produce the technically maximum output of agricultural produce obtained from the available resources (land, labor, machines, etc.. Price efficiency evaluates the ability of an agricultural sector to produce output of agricultural produce with regard to employing the optimal mix of resources given their prices. Because the theoretical ideal does not exist, efficiency of an agricultural sector, e.g. in Russia and of its fleet of agricultural machinery and tractors, is measured against agriculture of another country with maximum productivity efficiency of which is accepted as equal to unity or 100 percent. The calculation can be made on the basis of the Data Envelopment Analysis method. It is possible to calculate price and economic efficiency of Russian agriculture (fleet of agricultural machinery and tractors due to use its domestic prices.

Control Systems of Rubber Dryer Machinery Components Using Programmable Logic Control (PLC)

Science.gov (United States)

Hendra; Yulianto, A. S.; Indriani, A.; Hernadewita; Hermiyetti

2018-02-01

Application of programmable logic control (PLC) is widely used on the control systems in the many field engineering such as automotive, aviation, food processing and other industries [1-2]. PLC is simply program to control many automatic activity, easy to use, flexible and others. PLC using the ladder program to solve and regulated the control system component. In previous research, PLC was used for control system of rotary dryer machine. In this paper PLC are used for control system of motion component in the rubber dryer machinery. Component of rubber dryer machine is motors, gearbox, sprocket, heater, drying chamber and bearing. Principle working of rubber dryer machinery is wet rubber moving into the drying chamber by sprocket. Sprocket is driven by motors that conducted by PLC to moving and set of wet rubber on the drying chamber. Drying system uses greenhouse effect by making hanger dryer design in the form of line path. In this paper focused on motion control system motors and sensors drying rubber using PLC. The results show that control system of rubber dryer machinery can work in accordance control input and the time required to dry the rubber.

Vibration Feature Extraction and Analysis for Fault Diagnosis of Rotating Machinery-A Literature Survey

Directory of Open Access Journals (Sweden)

Saleem Riaz

2017-02-01

Full Text Available Safety, reliability, efficiency and performance of rotating machinery in all industrial applications are the main concerns. Rotating machines are widely used in various industrial applications. Condition monitoring and fault diagnosis of rotating machinery faults are very important and often complex and labor-intensive. Feature extraction techniques play a vital role for a reliable, effective and efficient feature extraction for the diagnosis of rotating machinery. Therefore, developing effective bearing fault diagnostic method using different fault features at different steps becomes more attractive. Bearings are widely used in medical applications, food processing industries, semi-conductor industries, paper making industries and aircraft components. This paper review has demonstrated that the latest reviews applied to rotating machinery on the available a variety of vibration feature extraction. Generally literature is classified into two main groups: frequency domain, time frequency analysis. However, fault detection and diagnosis of rotating machine vibration signal processing methods to present their own limitations. In practice, most healthy ingredients faulty vibration signal from background noise and mechanical vibration signals are buried. This paper also reviews that how the advanced signal processing methods, empirical mode decomposition and interference cancellation algorithm has been investigated and developed. The condition for rotating machines based rehabilitation, prevent failures increase the availability and reduce the cost of maintenance is becoming necessary too. Rotating machine fault detection and diagnostics in developing algorithms signal processing based on a key problem is the fault feature extraction or quantification. Currently, vibration signal, fault detection and diagnosis of rotating machinery based techniques most widely used techniques. Furthermore, the researchers are widely interested to make automatic

Part-time farmers and accidents with agricultural machinery: a moderated mediated model on the role played by frequency of use and unsafe beliefs.

Science.gov (United States)

Caffaro, Federica; Roccato, Michele; Micheletti Cremasco, Margherita; Cavallo, Eugenio

2018-01-25

We aimed at testing a model of the direct and indirect effects of being a part-time farmer on the probability of being involved in an agricultural machinery-related accident, considering the role played by unsafe beliefs and the frequency of use of machinery. Two-hundred and fifty-two Italian men, regular users of agricultural machinery (age: Mean = 45.1 years, standard Deviation = 17.5), were administered a paper-and-pencil questionnaire addressing their relation with work, unsafe beliefs, and previous experience of machinery-related accidents. Being a part-time farmer showed a positive association with unsafe beliefs only among occasional machinery users. Unsafe beliefs in turn showed a positive association with accidents. The study gave a novel contribution to the knowledge of the chain of events connecting part-time farmers with machinery-related accidents. Preventive training interventions targeting part-timer farmers using agricultural machinery just occasionally should be developed.

2006 China Machinery and Electronical Products Trade Fair:Ample Fruits Shown

Institute of Scientific and Technical Information of China (English)

无

2006-01-01

@@ 2006 Malaysia China Machinery and Electronical Products Trade Fair, organized by China Council for the Promotion of International Trade(CCPIT), was held in August in Prince World Trade Center(PWTC), Kuala Lumpur.

Research on prediction of agricultural machinery total power based on grey model optimized by genetic algorithm

Science.gov (United States)

Xie, Yan; Li, Mu; Zhou, Jin; Zheng, Chang-zheng

2009-07-01

Agricultural machinery total power is an important index to reflex and evaluate the level of agricultural mechanization. It is the power source of agricultural production, and is the main factors to enhance the comprehensive agricultural production capacity expand production scale and increase the income of the farmers. Its demand is affected by natural, economic, technological and social and other "grey" factors. Therefore, grey system theory can be used to analyze the development of agricultural machinery total power. A method based on genetic algorithm optimizing grey modeling process is introduced in this paper. This method makes full use of the advantages of the grey prediction model and characteristics of genetic algorithm to find global optimization. So the prediction model is more accurate. According to data from a province, the GM (1, 1) model for predicting agricultural machinery total power was given based on the grey system theories and genetic algorithm. The result indicates that the model can be used as agricultural machinery total power an effective tool for prediction.

Implications of material selection on the design of packaging machinery.

Science.gov (United States)

Merritt, J P

2009-01-01

Material selection has significant implications on the design and cost of horizontal-form-fill-seal packaging machinery. To avoid excessive costs, machine redesigns and project delays, material selection must be reconciled early in the project and revisited throughout the construction of the machine.

Scattering transform and LSPTSVM based fault diagnosis of rotating machinery

Science.gov (United States)

Ma, Shangjun; Cheng, Bo; Shang, Zhaowei; Liu, Geng

2018-05-01

This paper proposes an algorithm for fault diagnosis of rotating machinery to overcome the shortcomings of classical techniques which are noise sensitive in feature extraction and time consuming for training. Based on the scattering transform and the least squares recursive projection twin support vector machine (LSPTSVM), the method has the advantages of high efficiency and insensitivity for noise signal. Using the energy of the scattering coefficients in each sub-band, the features of the vibration signals are obtained. Then, an LSPTSVM classifier is used for fault diagnosis. The new method is compared with other common methods including the proximal support vector machine, the standard support vector machine and multi-scale theory by using fault data for two systems, a motor bearing and a gear box. The results show that the new method proposed in this study is more effective for fault diagnosis of rotating machinery.

THE ROLE OF POSTHARVEST MACHINERIES AND PACKAGING IN MINIMIZING AFLATOXIN CONTAMINATION IN PEANUT

Directory of Open Access Journals (Sweden)

Raffi Paramawati

2016-10-01

Full Text Available As a tropical country with relatively high humidity and temperature, Indonesia is struggling with aflatoxin which frequently contaminates peanut. Aflatoxin is a carcinogenic toxic substance that could cause liver cancer. Due to the increasing concern on food safety, the Indonesian Drugs and Foods Agency specifies the maximum aflatoxin allowed in peanut as much as 20 ppb. However, researches showed that aflatoxin contamination in peanut in Indonesia is much higher than the threshold. The study was carried out to observe the effect of using postharvest machineries and packaging  treatments on aflatoxin contamination in peanut. Reduction of postharvest processes was conducted by using series of machineries, e.g. thresher, dryer, and sheller. Packaging treatments, e.g. vacuum plastic pack, hermetic glass chamber, and polyethylene (PE plastic wrap were carried out during storage at ambient temperature (25-27°C. The results showed that using machineries in postharvest handling produced peanut free from aflatoxin contamination. However, without effective packaging, the aflatoxin level would increase during storage. Hermetic packaging could protect peanut from the mold as indicated by low level of aflatoxin contamination.

Large-Scale Analysis Exploring Evolution of Catalytic Machineries and Mechanisms in Enzyme Superfamilies.

Science.gov (United States)

Furnham, Nicholas; Dawson, Natalie L; Rahman, Syed A; Thornton, Janet M; Orengo, Christine A

2016-01-29

Enzymes, as biological catalysts, form the basis of all forms of life. How these proteins have evolved their functions remains a fundamental question in biology. Over 100 years of detailed biochemistry studies, combined with the large volumes of sequence and protein structural data now available, means that we are able to perform large-scale analyses to address this question. Using a range of computational tools and resources, we have compiled information on all experimentally annotated changes in enzyme function within 379 structurally defined protein domain superfamilies, linking the changes observed in functions during evolution to changes in reaction chemistry. Many superfamilies show changes in function at some level, although one function often dominates one superfamily. We use quantitative measures of changes in reaction chemistry to reveal the various types of chemical changes occurring during evolution and to exemplify these by detailed examples. Additionally, we use structural information of the enzymes active site to examine how different superfamilies have changed their catalytic machinery during evolution. Some superfamilies have changed the reactions they perform without changing catalytic machinery. In others, large changes of enzyme function, in terms of both overall chemistry and substrate specificity, have been brought about by significant changes in catalytic machinery. Interestingly, in some superfamilies, relatives perform similar functions but with different catalytic machineries. This analysis highlights characteristics of functional evolution across a wide range of superfamilies, providing insights that will be useful in predicting the function of uncharacterised sequences and the design of new synthetic enzymes. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

Electric machinery and drives in thermal power stations

International Nuclear Information System (INIS)

1974-01-01

The following subjects were dealt with during the VDE meeting: 1) Requirements made by the electric network on the generators and their excitation equipment, and the influence thereof on their design; 2) requirements made by the power station process on the electric drives and the influence thereof on type and design; 3) requirements made on protective measures from the point of the electric power station machinery. (TK) [de

Targeting Transcription Elongation Machinery for Breast Cancer Therapy

Science.gov (United States)

2017-05-01

ABSTRACT: This project focuses on the important but under-studied role of the P-TEFb- dependent transcription elongation machinery in human breast...molecule CDK9 inhibitors can be used to halt breast cancer metastasis. 8 experimental groups to test various drug dosage and frequency regimes will...tumor cells, which are said to be ’ addicted ’ to this protein. Consistently, pharmacological inhibition of Hsp90 has demonstrated great promise in

Progress in control equipment for fuel-handling machinery

International Nuclear Information System (INIS)

Nutting, B.A.

1986-01-01

The paper outlines the development of the equipment used to control the fuel-handling machinery associated with nuclear reactors, from the early electromechanical equipment, through solid-state switching logic to programmable controllers and microprocessors. The control techniques have developed along with the technology, and modern systems offer versatility, reliability and ease of design, operation and maintenance. Future trends and developments are discussed together with possible limiting factors. (author)

Application of logical analysis of data to machinery-related accident prevention based on scarce data

International Nuclear Information System (INIS)

Jocelyn, Sabrina; Chinniah, Yuvin; Ouali, Mohamed-Salah; Yacout, Soumaya

2017-01-01

This paper deals with the application of Logical Analysis of Data (LAD) to machinery-related occupational accidents, using belt-conveyor-related accidents as an example. LAD is a pattern recognition and classification approach. It exploits the advancement in information technology and computational power in order to characterize the phenomenon under study. The application of LAD to machinery-related accident prevention is innovative. Ideally, accidents do not occur regularly, and as a result, companies have little data about them. The first objective of this paper is to demonstrate the feasibility of using LAD as an algorithm to characterize a small sample of machinery-related accidents with an adequate average classification accuracy. The second is to show that LAD can be used for prevention of machinery-related accidents. The results indicate that LAD is able to characterize different types of accidents with an average classification accuracy of 72–74%, which is satisfactory when compared with other studies dealing with large amounts of data where such a level of accuracy is considered adequate. The paper shows that the quantitative information provided by LAD about the patterns generated can be used as a logical way to prioritize risk factors. This prioritization helps safety practitioners make decisions regarding safety measures for machines. - Highlights: • LAD is presented as an innovative approach to prevent machinery-related accidents. • LAD is applied to a very small database of belt-conveyor-related accidents. • Despite scarce data, LAD generates patterns with adequate classification accuracy. • The patterns characterize different types of belt-conveyor-related accidents. • The patterns are useful to belt conveyor risk identification and risk estimation.

EXPLORING LINKS AMONG INVENTORY AND FINANCIAL PERFORMANCE IN THE AGRICULTURAL MACHINERY INDUSTRY

Directory of Open Access Journals (Sweden)

Dimitris Folinas

2014-10-01

Full Text Available The paper aims to investigate the effects of Inventory Turnover and Inventory Days on firm performance in the United Kingdom agricultural machinery industry by examining past literature reviews and empirical evidence of a primary research. Specific performance measures such as Earnings before Interest and Tax to Sales Ratio, Gross Profit to Sales Ratio, and Return on Assets are examined by conducting statistical analyses to determine the correlations between inventory and financial performance in agricultural machinery industry. The analysis of Inventory Turnover with financial performance measures doesn’t indicate any links between these variables. Furthermore, based on the results, Inventory Days plays a role in the financial performance of organisations however to varying degrees.

FOREWORD: The XXV IAHR Symposium on Hydraulic Machinery and Systems marks half a century tradition

Science.gov (United States)

Susan-Resiga, Romeo

2010-05-01

IAHR75_logoUPT90_logoARFT_logo International Association of Hydro-Environment Engineering and Research'Politehnica' University of TimisoaraRomanian Academy - Timisoara Branch The 25th edition of the IAHR Symposium on Hydraulic Machinery and Systems, held in Timisoara, Romania, 20-24 September 2010, jointly organized by the 'Politehnica' University of Timisoara and the Romanian Academy - Timisoara Branch, marks a half century tradition of these prestigious symposia. However, it is the first time that Romania hosts such a symposium, and for good reasons. The Romanian electrical power system has a total of 20,630 MW installed power, out of which 6,422 MW in hydropower plants. The energy produced in hydropower facilities was in 2008 of 17,105 GWh from a total of 64,772 GWh electrical energy production. Moreover, for the period 2009-2015, new hydropower capacities are going to be developed, with a total of 2,157 MW installed power and an estimated 5,770 GWh/year energy production. Within the same period of time, the refurbishment, modernization and repair programs will increase the actual hydropower production with an estimated 349 GWh/year. The 'Politehnica' University of Timisoara is proud to host the 25th IAHR Symposium on Hydraulic Machinery and Systems, in the year of its 90th anniversary. The 'Politehnica' University of Timisoara is one of the largest and most well-known technical universities from Central and Eastern Europe. It was founded in 1920, a short time after the union into one state of all the Romanian territories, following the end of the First World War, in order to respond to the need engineers felt by the Romanian society at that time, within the economical development framework. During its 90 years of existence, 'Politehnica' University of Timisoara educated over 100,000 engineers, greatly appreciated both in Romania and abroad, for their competence and seriousness. King Ferdinand I of Romania said while visiting the recently established

Designing a machinery control system (MCS) security testbed

OpenAIRE

Desso, Nathan H.

2014-01-01

Approved for public release; distribution is unlimited Industrial control systems (ICS) face daily cyber security threats, can have a significant impact to the security of our nation, and present a difficult challenge to defend. Critical infrastructures, including military systems like the machinery control systems (MCS) found onboard modern U.S. warships, are affected because of their use of commercial automation solutions. The increase of automated control systems within the U.S. Navy sa...

Fault diagnosis in rotating machinery by vibration analysis

International Nuclear Information System (INIS)

Behzad, M.; Asayesh, M.

2002-01-01

Dynamic behavior of unbalanced bent shaft has been investigated in this research. Finite element method is used for unbalance response calculation of a bent shaft. The result shows the effect of bent on the unbalance response. The angle between bent vector and unbalance force, position and type of supports, shaft diameter and disk position can affect the outcome. The results of this research can significantly help in fault diagnosis in rotating machinery

Analysis of electric machinery and drive systems

CERN Document Server

Krause, Paul C; Sudhoff, Scott D; Pekarek, Steven

2013-01-01

Introducing a new edition of the popular reference on machine analysis Now in a fully revised and expanded edition, this widely used reference on machine analysis boasts many changes designed to address the varied needs of engineers in the electric machinery, electric drives, and electric power industries. The authors draw on their own extensive research efforts, bringing all topics up to date and outlining a variety of new approaches they have developed over the past decade. Focusing on reference frame theory that has been at the core of this work since the first edition, th

Electrification of agricultural machinery; Elektrifizierung von Landmaschinen

Energy Technology Data Exchange (ETDEWEB)

Goetz, Manuel; Grad, Karl; Weinmann, Olrik [ZF Friedrichshafen AG, Friedrichshafen (Germany)

2012-10-15

As early as 2009, ZF exhibited a generator system for agricultural machinery at Agritechnica under the name Terra+ which consisted of an electric motor in the transmission housing. As part of the ElecTra project, the company is now presenting its first tractor prototype with this generator system at Agritechnica 2011. The project involves combining the electrification of the tractor's auxiliary systems with electric drives for an attachment. The electrification of the implement was carried out in cooperation with Amazone, manufacturer of implements for the agricultural industry. (orig.)

The Machinery for Enforcement of Domestic Arbitral Awards in Nigeria

African Journals Online (AJOL)

Nnamdi Azikiwe University Journal of International Law and Jurisprudence ... Arbitration is a private means of resolving dispute which is resorted to, chiefly because the parties choose to avoid as much as possible employing the state machinery for dispute resolution, namely the court and its dreaded time consuming ...

Guidelines for meeting emission regulations for rotating machinery with mechanical seals

Science.gov (United States)

Mechanical seals have played a major role during many years in minimizing emissions to atmosphere in rotating machinery. A review is presented of the continuing innovations and new technologies to improve mechanical seal performance in terms of emissions.

Industry sector analysis, Mexico: Oil and gas field machinery and equipment. Export Trade Information

International Nuclear Information System (INIS)

1990-04-01

The Industry Sector Analyses (I.S.A.) for oil and gas field machinery and equipment contains statistical and narrative information on projected market demand, end-users, receptivity of Mexican consumers to U.S. products, the competitive situation - Mexican production, total import market, U.S. market position, foreign competition, and competitive factors, and market access - Mexican tariffs, non-tariff barriers, standards, taxes and distribution channels. The I.S.A. provides the United States industry with meaningful information regarding the Mexican market for oil and gas field machinery and equipment

The iron-sulfur cluster assembly machineries in plants: current knowledge and open questions

Science.gov (United States)

Couturier, Jérémy; Touraine, Brigitte; Briat, Jean-François; Gaymard, Frédéric; Rouhier, Nicolas

2013-01-01

Many metabolic pathways and cellular processes occurring in most sub-cellular compartments depend on the functioning of iron-sulfur (Fe-S) proteins, whose cofactors are assembled through dedicated protein machineries. Recent advances have been made in the knowledge of the functions of individual components through a combination of genetic, biochemical and structural approaches, primarily in prokaryotes and non-plant eukaryotes. Whereas most of the components of these machineries are conserved between kingdoms, their complexity is likely increased in plants owing to the presence of additional assembly proteins and to the existence of expanded families for several assembly proteins. This review focuses on the new actors discovered in the past few years, such as glutaredoxin, BOLA and NEET proteins as well as MIP18, MMS19, TAH18, DRE2 for the cytosolic machinery, which are integrated into a model for the plant Fe-S cluster biogenesis systems. It also discusses a few issues currently subjected to an intense debate such as the role of the mitochondrial frataxin and of glutaredoxins, the functional separation between scaffold, carrier and iron-delivery proteins and the crosstalk existing between different organelles. PMID:23898337

Application of Functional Link Artificial Neural Network for Prediction of Machinery Noise in Opencast Mines

Directory of Open Access Journals (Sweden)

Santosh Kumar Nanda

2011-01-01

Full Text Available Functional link-based neural network models were applied to predict opencast mining machineries noise. The paper analyzes the prediction capabilities of functional link neural network based noise prediction models vis-à-vis existing statistical models. In order to find the actual noise status in opencast mines, some of the popular noise prediction models, for example, ISO-9613-2, CONCAWE, VDI, and ENM, have been applied in mining and allied industries to predict the machineries noise by considering various attenuation factors. Functional link artificial neural network (FLANN, polynomial perceptron network (PPN, and Legendre neural network (LeNN were used to predict the machinery noise in opencast mines. The case study is based on data collected from an opencast coal mine of Orissa, India. From the present investigations, it could be concluded that the FLANN model give better noise prediction than the PPN and LeNN model.

Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes.

Science.gov (United States)

Iwasaki, Shintaro; Kobayashi, Maki; Yoda, Mayuko; Sakaguchi, Yuriko; Katsuma, Susumu; Suzuki, Tsutomu; Tomari, Yukihide

2010-07-30

Small silencing RNAs--small interfering RNAs (siRNAs) or microRNAs (miRNAs)--direct posttranscriptional gene silencing of their mRNA targets as guides for the RNA-induced silencing complex (RISC). Both siRNAs and miRNAs are born double stranded. Surprisingly, loading these small RNA duplexes into Argonaute proteins, the core components of RISC, requires ATP, whereas separating the two small RNA strands within Argonaute does not. Here we show that the Hsc70/Hsp90 chaperone machinery is required to load small RNA duplexes into Argonaute proteins, but not for subsequent strand separation or target cleavage. We envision that the chaperone machinery uses ATP and mediates a conformational opening of Ago proteins so that they can receive bulky small RNA duplexes. Our data suggest that the chaperone machinery may serve as the driving force for the RISC assembly pathway. Copyright 2010 Elsevier Inc. All rights reserved.

DIESEL ENGINE SYSTEMS. AGRICULTURAL MACHINERY--SERVICE OCCUPATIONS, MODULE NUMBER 15.

Science.gov (United States)

Ohio State Univ., Columbus. Center for Vocational and Technical Education.

ONE OF A SERIES DESIGNED TO HELP TEACHERS PREPARE POSTSECONDARY STUDENTS FOR AGRICULTURAL MACHINERY SERVICE OCCUPATIONS AS PARTS MEN, MECHANICS, MECHANIC'S HELPERS, AND SERVICE SUPERVISORS, THIS GUIDE AIMS TO DEVELOP STUDENT UNDERSTANDING OF THE CONSTRUCTION AND OPERATING PRINCIPLES OF DIESEL ENGINES. IT WAS DEVELOPED BY A NATIONAL TASK FORCE ON…

Updating the model TREMOD - Mobile Machinery (TREMOD-MM); Aktualisierung des Modells TREMOD - Mobile Machinery (TREMOD-MM)

Energy Technology Data Exchange (ETDEWEB)

Helms, Hinrich; Lambrecht, Udo; Knoerr, Wolfram [ifeu - Institut fuer Energie- und Umweltforschung Heidelberg gGmbH, Heidelberg (Germany)

2010-05-15

In the context of the project ''Development of a model for the computation of the air pollutant emissions and the fuel consumption of combustion engines in mobile devices and machines'', the Institute for Energy and Environmental Research GmbH (Heidelberg, Federal Republic of Germany) has created the model TREMOD-MM (TREMOD Mobile Machinery). Thus a detailed computation of the emissions from mobile devices and machines in the agriculture, construction industry, forestry and gardening as well as the sport shipping and passenger shipping can be accomplished. Strongly differentiated data are considered to the age structure, engine performance, use and emission behaviour. Thus it is possible to compute the emissions for different scenarios in high degree of detail.

Hybridisation of powertrains for construction machinery; Hybridisierung von Antriebsstraengen fuer Baumaschinen

Energy Technology Data Exchange (ETDEWEB)

Mohr, Mark; Goetz, Manuel; Fellmann, Martin [ZF Friedrichshafen AG (Germany). Vorentwicklung Arbeitsmaschinen-Antriebe; Brehmer, Udo [ZF Passau GmbH (Germany). Entwicklung Baumaschinengetriebe

2010-04-15

Increasing fuel prices and increased environmental requirements lead, for construction machinery, to an increased importance of measures to reduce fuel consumption. The hybrid drive in this sector could become an economical option by using synergies for example with the commercial vehicle section. (orig.)

Energy-autonomous wireless vibration sensor for condition-based maintenance of machinery

NARCIS (Netherlands)

Wang, Z.; Bouwens, F.; Vullers, R.; Petré, F.; Devos, S.

2011-01-01

This paper addresses the development of an energy-autonomous wireless vibration sensor for condition-based monitoring of machinery. Such technology plays an increasingly important role in modern manufacturing industry. In this work, energy harvesting is realized by resorting to a custom designed

Cell secretion machinery: Studies using the AFM

International Nuclear Information System (INIS)

Jena, Bhanu P.

2006-01-01

A new field in biology, 'nano-cell biology', has emerged from the successful use of force microscopy in understanding the structure and dynamics of cells and biomolecules, at nm resolution and in real time. Atomic force microscopy, in combination with conventional tools and approaches (electron microscopy, electrophysiology, X-ray diffraction, photon correlation spectroscopy, mass spectroscopy, biochemistry, and molecular biology), has revealed for the first time, the universal molecular machinery and mechanism of secretion in cells. Secretion occurs in all living cells and involves the delivery of intracellular products to the cell exterior. Secretory products are packaged and stored in membranous sacs or vesicles within the cell. When the cell needs to secrete these products, the secretory vesicles containing them, dock and fuse at plasma membrane-associated supramolecular structures called Porosome, to release their contents. Specialized cells for neurotransmission, enzyme secretion, or hormone release utilize a highly regulated secretory process. During secretion, swelling of secretory vesicles results in a build-up of intravesicular pressure, allowing expulsion of vesicular contents. The extent of vesicle swelling dictates the amount of vesicular contents expelled. The discovery of the porosome as the universal secretory machinery, its isolation, its structure and dynamics at nm resolution and in real time, its biochemical composition and functional reconstitution into artificial lipid membrane, have been determined. The molecular mechanism of secretory vesicle swelling, and the fusion of opposing bilayers, i.e., the fusion of secretory vesicle membrane at the base of the porosome membrane, has also been resolved

Motion control of multi-actuator hydraulic systems for mobile machineries: Recent advancements and future trends

Science.gov (United States)

Xu, Bing; Cheng, Min

2018-06-01

This paper presents a survey of recent advancements and upcoming trends in motion control technologies employed in designing multi-actuator hydraulic systems for mobile machineries. Hydraulic systems have been extensively used in mobile machineries due to their superior power density and robustness. However, motion control technologies of multi-actuator hydraulic systems have faced increasing challenges due to stringent emission regulations. In this study, an overview of the evolution of existing throttling control technologies is presented, including open-center and load sensing controls. Recent advancements in energy-saving hydraulic technologies, such as individual metering, displacement, and hybrid controls, are briefly summarized. The impact of energy-saving hydraulic technologies on dynamic performance and control solutions are also discussed. Then, the advanced operation methods of multi-actuator mobile machineries are reviewed, including coordinated and haptic controls. Finally, challenges and opportunities of advanced motion control technologies are presented by providing an overall consideration of energy efficiency, controllability, cost, reliability, and other aspects.

Robust IFE Based Order Analysis of Rotating Machinery in Virtual Instrument

International Nuclear Information System (INIS)

Guo, Y; Chi, Y L; Huang, Y Y; Qin, S R

2006-01-01

Character analysis plays an important role in fault-find and diagnosis of rotating machinery. Order analysis is one of the major methods in character analysis for the analysis of non-stationary vibration signals in run-up or coast down of rotating machinery. An order analysis method, which employs instantaneous frequency estimation based on time-frequency analysis, is introduced. In contrast with traditional order analysis methods, this method avoids the use of tachometer and other special hardware; hence it makes the application of order analysis simplified. The order analysis introduced in the paper with the character that only software is depended for order tracking makes it specially satisfy the requirement of Virtual Instruments. Corresponding order analysis items, such as order spectrum, order spectrum matrix and tracking order spectrum etc., which are applied in Virtual Instruments, are also introduced. A test example is provided to demonstrate the validity of the method presented

Research on monitoring technology of axial gap change about high-speed rotating machinery

International Nuclear Information System (INIS)

Zhang Xiaochan; Liu Fanglei; Hu Shihua; Xie Qing; Li Zhen

2014-01-01

This paper describes that the only measuring point of high-speed rotating machinery (speed monitoring transducer) measuring the operation of the axial gap change and application. According to mechanism analysis the speed monitoring transducer's signal, prove its amplitude changes including the axial gap change information. To carry out the speed monitoring transducer qualitative and quantitative axial gap change research, Find the output signal amplitude and clearance change corresponding relationship formula of speed monitoring transducer, define the measurement method. Based on the above analsis, manufacture the single channel measurement devices and multiple unit measurement system, provide an important fault decision of high-speed rotating machinery, it can be applied to new equipment development and production. (authors)

Fault Severity Estimation of Rotating Machinery Based on Residual Signals

Directory of Open Access Journals (Sweden)

Fan Jiang

2012-01-01

Full Text Available Fault severity estimation is an important part of a condition-based maintenance system, which can monitor the performance of an operation machine and enhance its level of safety. In this paper, a novel method based on statistical property and residual signals is developed for estimating the fault severity of rotating machinery. The fast Fourier transformation (FFT is applied to extract the so-called multifrequency-band energy (MFBE from the vibration signals of rotating machinery with different fault severity levels in the first stage. Usually these features of the working conditions with different fault sensitivities are different. Therefore a sensitive features-selecting algorithm is defined to construct the feature matrix and calculate the statistic parameter (mean in the second stage. In the last stage, the residual signals computed by the zero space vector are used to estimate the fault severity. Simulation and experimental results reveal that the proposed method based on statistics and residual signals is effective and feasible for estimating the severity of a rotating machine fault.

Effects of high-gradient magnetic fields on living cell machinery

International Nuclear Information System (INIS)

Zablotskii, V; Lunov, O; Kubinova, S; Polyakova, T; Dejneka, A; Sykova, E

2016-01-01

A general interest in biomagnetic effects is related to fundamental studies of the influence of magnetic fields on living objects on the cellular and whole organism levels. Emerging technologies offer new directions for the use of high-gradient magnetic fields to control cell machinery and to understand the intracellular biological processes of the emerging field of nanomedicine. In this review we aim at highlighting recent advances made in identifying fundamental mechanisms by which magnetic gradient forces act on cell fate specification and cell differentiation. The review also provides an analysis of the currently available magnetic systems capable of generating magnetic fields with spatial gradients of up to 10 MT m −1 , with the focus on their suitability for use in cell therapy. Relationships between experimental factors and underlying biophysical mechanisms and assumptions that would ultimately lead to a deeper understanding of cell machinery and the development of more predictive models for the evaluation of the effects of magnetic fields on cells, tissue and organisms are comprehensively discussed. (topical review)

Vibration sensor data denoising using a time-frequency manifold for machinery fault diagnosis.

Science.gov (United States)

He, Qingbo; Wang, Xiangxiang; Zhou, Qiang

2013-12-27

Vibration sensor data from a mechanical system are often associated with important measurement information useful for machinery fault diagnosis. However, in practice the existence of background noise makes it difficult to identify the fault signature from the sensing data. This paper introduces the time-frequency manifold (TFM) concept into sensor data denoising and proposes a novel denoising method for reliable machinery fault diagnosis. The TFM signature reflects the intrinsic time-frequency structure of a non-stationary signal. The proposed method intends to realize data denoising by synthesizing the TFM using time-frequency synthesis and phase space reconstruction (PSR) synthesis. Due to the merits of the TFM in noise suppression and resolution enhancement, the denoised signal would have satisfactory denoising effects, as well as inherent time-frequency structure keeping. Moreover, this paper presents a clustering-based statistical parameter to evaluate the proposed method, and also presents a new diagnostic approach, called frequency probability time series (FPTS) spectral analysis, to show its effectiveness in fault diagnosis. The proposed TFM-based data denoising method has been employed to deal with a set of vibration sensor data from defective bearings, and the results verify that for machinery fault diagnosis the method is superior to two traditional denoising methods.

DETERMINATION OF AGRICULTURAL MACHINERY OPERATORS’ OPINIONS ABOUT THE CABIN COMFORT IN ESKİŞEHİR

Directory of Open Access Journals (Sweden)

Özge ACARBAŞ BALTACI

2015-08-01

Full Text Available Comfort has a great importance in the interior design of tractor and agricultural machinery cabins. Operators are exposed to muscoskeletal system disorders since they spend long time periods during the day in these vehicles. There is a few work in the literature reporting operators’ opinions about cabin comfort of these machineries. In this study, a questionnaire was conducted in order to get information about agricultural machinery operators’ opinions about the comfort of their vehicles. Tractor cabins and combine harvester machine cabins were selected as machineries. The study was conducted in Eskişehir in Turkey. Questionnaire was composed of four groups of questions and five ordered response levels were used in the Likert's scale. Demographic questions, general questions about the machine, personal evaluation questions and open ended questions were asked to the operators. After the questionnaire completed, collected data were classified according to the machine type. Frequency tables were used to present the results. Visibility and the accessibility were the most satisfied issues for the tractor operators with 55.9% and 55.4% percentages, respectively. Seat comfort has the highest satisfaction degree with 43.7% for the combine harvester operators. Cronbach Alpha reliability coefficient was used for the satisfaction questions in the applied questionnaire. The reliability of the study was high with coefficients of 0.878 and 0.940 for the tractor and combine harvester questionnaires, respectively. This study will support design and development process of new products by considering operator opinions.

Conflicting views on the membrane fusion machinery and the fusion pore

DEFF Research Database (Denmark)

Sørensen, Jakob B

2009-01-01

of the assembly of the fusogenic SNARE-complex. Here, I review conflicting views on the function of the core fusion machinery consisting of the SNAREs, Munc18, complexin, and synaptotagmin. Munc18 controls docking of vesicles to the plasma membrane and initial SNARE-complex assembly, whereas complexin...

Technical development of fluid machinery area

International Nuclear Information System (INIS)

Chung, Kyung Nam; Kim, Jin Young; Kim, Yang Ik

2008-01-01

In this paper, recent research activity of Hyundai Heavy Industries in the fluid machinery area is introduced. Technical development has been carried out in pumps, turbines, construction equipment, side thrusters, engine lubrication flow, etc. Here the technology of pumps and cooling of construction equipment will be dealt with. We have actively used computational fluid dynamics in the performance analysis of pump models and the design of new models of various industrial pumps or marine pumps. And a cooling analysis system composed of 3D flow analysis and 1D cooling analysis has been established, and applied to the design of cooling systems of new models of excavators and wheel loaders. The above mentioned technology is presented in details, and some future works are mentioned

Recent Advances in Precision Machinery and Manufacturing Technology

DEFF Research Database (Denmark)

Liu, Chien-Hung; Hsieh, Wen-Hsiang; Chang, Zong-Yu

2014-01-01

Precision machinery and manufacturing technology are be- coming more important in current and future technologies. New knowledge in this field will aid in the advancement of various technologies that are needed to gain industrial competitiveness. To this end, the special issue aims to disseminate...... the latest advancements of relevant fundamental and applied research works of high quality to the inter- national community. The topics of the accepted articles in the special issue include precision manufacturing pro- cesses, measurements and control, robotics and automation, machine tools, advanced...

A Novel Fault Diagnosis Method for Rotating Machinery Based on a Convolutional Neural Network.

Science.gov (United States)

Guo, Sheng; Yang, Tao; Gao, Wei; Zhang, Chen

2018-05-04

Fault diagnosis is critical to ensure the safety and reliable operation of rotating machinery. Most methods used in fault diagnosis of rotating machinery extract a few feature values from vibration signals for fault diagnosis, which is a dimensionality reduction from the original signal and may omit some important fault messages in the original signal. Thus, a novel diagnosis method is proposed involving the use of a convolutional neural network (CNN) to directly classify the continuous wavelet transform scalogram (CWTS), which is a time-frequency domain transform of the original signal and can contain most of the information of the vibration signals. In this method, CWTS is formed by discomposing vibration signals of rotating machinery in different scales using wavelet transform. Then the CNN is trained to diagnose faults, with CWTS as the input. A series of experiments is conducted on the rotor experiment platform using this method. The results indicate that the proposed method can diagnose the faults accurately. To verify the universality of this method, the trained CNN was also used to perform fault diagnosis for another piece of rotor equipment, and a good result was achieved.

Outer membrane targeting of Pseudomonas aeruginosa proteins shows variable dependence on the components of Bam and Lol machineries.

Science.gov (United States)

Hoang, Hanh H; Nickerson, Nicholas N; Lee, Vincent T; Kazimirova, Anastasia; Chami, Mohamed; Pugsley, Anthony P; Lory, Stephen

2011-01-01

In Gram-negative bacteria, the Lol and Bam machineries direct the targeting of lipidated and nonlipidated proteins, respectively, to the outer membrane (OM). Using Pseudomonas aeruginosa strains with depleted levels of specific Bam and Lol proteins, we demonstrated a variable dependence of different OM proteins on these targeting pathways. Reduction in the level of BamA significantly affected the ability of the β-barrel membrane protein OprF to localize to the OM, while the targeting of three secretins that are functionally related OM proteins was less affected (PilQ and PscC) or not at all affected (XcpQ). Depletion of LolB affected all lipoproteins examined and had a variable effect on the nonlipidated proteins. While the levels of OprF, PilQ, and PscC were significantly reduced by LolB depletion, XcpQ was unaffected and was correctly localized to the OM. These results suggest that certain β-barrel proteins such as OprF primarily utilize the complete Bam machinery. The Lol machinery participates in the OM targeting of secretins to variable degrees, likely through its involvement in the assembly of lipidated Bam components. XcpQ, but not PilQ or PscC, was shown to assemble spontaneously into liposomes as multimers. This work raises the possibility that there is a gradient of utilization of Bam and Lol insertion and targeting machineries. Structural features of individual proteins, including their β-barrel content, may determine the propensity of these proteins for folding (or misfolding) during periplasmic transit and OM insertion, thereby influencing the extent of utilization of the Bam targeting machinery, respectively. Targeting of lipidated and nonlipidated proteins to the outer membrane (OM) compartment in Gram-negative bacteria involves the transfer across the periplasm utilizing the Lol and Bam machineries, respectively. We show that depletion of Bam and Lol components in Pseudomonas aeruginosa does not lead to a general OM protein translocation defect

29 CFR 1918.98 - Qualifications of machinery operators and supervisory training.

Science.gov (United States)

2010-07-01

...) Qualification of machinery operators. (1) Only an employee determined by the employer to be competent by reason...). Therefore, employers who act in accordance with the employment provisions (Title I) of the ADA (42 U.S.C... authority; elements of accidents prevention; attitudes, leadership and motivation; hazards of longshoring...

Dedicated monitoring and machinery protection systems on reciprocating compressors

Energy Technology Data Exchange (ETDEWEB)

Grande, Alvaro; Wenisch, Markus [Hoerbiger Ventilwerke GmbH and Co KG, Wien (Austria); Jacobs, Denis [HOERBIGER do Brasil Industria de Equipamentos, Cajamar, SP (Brazil)

2012-07-01

Growing demands on reciprocating compressors (recips) in the process gas industry require particular solutions for machinery protection and performance monitoring systems. Compared to rotating equipment, monitoring systems for recips have to consider the special mechanical and physical characteristics, such as oscillating masses, variable vibration behaviour and varying operating conditions. Furthermore, they provide valuable information about the performance of cylinder related components allowing the operator the optimization of efficiency and availability, and therefore increase production. (author)

Fault diagnosis of rotating machinery using an improved HHT based on EEMD and sensitive IMFs

International Nuclear Information System (INIS)

Lei, Yaguo; Zuo, Ming J

2009-01-01

A Hilbert–Huang transform (HHT) is a time–frequency technique and has been widely applied to analyzing vibration signals in the field of fault diagnosis of rotating machinery. It analyzes the vibration signals using intrinsic mode functions (IMFs) extracted using empirical mode decomposition (EMD). However, EMD sometimes cannot reveal the signal characteristics accurately because of the problem of mode mixing. Ensemble empirical mode decomposition (EEMD) was developed recently to alleviate this problem. The IMFs generated by EEMD have different sensitivity to faults. Some IMFs are sensitive and closely related to the faults but others are irrelevant. To enhance the accuracy of the HHT in fault diagnosis of rotating machinery, an improved HHT based on EEMD and sensitive IMFs is proposed in this paper. Simulated signals demonstrate the effectiveness of the improved HHT in diagnosing the faults of rotating machinery. Finally, the improved HHT is applied to diagnosing an early rub-impact fault of a heavy oil catalytic cracking machine set, and the application results prove that the improved HHT is superior to the HHT based on all IMFs of EMD

Abrasive Wear of Alloyed Cast Steels Applied for Heavy Machinery

Directory of Open Access Journals (Sweden)

Studnicki A.

2015-03-01

Full Text Available In the paper the results and analysis of abrasive wear studies were shown for two grades of cast steels: low-alloyed cast steel applied for heavy machinery parts such as housing, covers etc. and chromium cast steels applied for kinetic nodes of pin-sleeve type. Studies were performed using the modified in Department of Foundry pin-on-disc method.

MODELING ENVIRONMENTAL IMPACT OF MACHINERY SECTORS TO PROMOTE SUSTAINABLE DEVELOPMENT OF THAILAND

Directory of Open Access Journals (Sweden)

Pruethsan Sutthichaimethee

2016-01-01

Full Text Available The objective of this research is to propose an indicator to evaluate environmental impacts from the machinery sectors of Thailand, leading to more sustainable consumption and production in this sector of the economy. The factors used to calculate the forward linkage, backward linkage and real benefit included the total environmental costs. The highest total environmental cost was railway equipment need to be resolved immediately because it uses natural resources in carrying capacity, higher than standard environmental cost, and contribute to low real benefit. Electric accumulator & battery, secondary special industrial machinery, motorcycle, bicycle & other carriages, and engines and turbines need to monitor closely because they are able to link to other production sectors more than other production sector do and they have high environmental cost. In order to decide the sustainable development strategy of the country, there is a need to use this research to support decision-making.

Identification and analysis of the RNA degrading complexes and machinery of Giardia lamblia using an in silico approach.

Science.gov (United States)

Williams, Christopher W; Elmendorf, Heidi G

2011-11-29

RNA degradation is critical to the survival of all cells. With increasing evidence for pervasive transcription in cells, RNA degradation has gained recognition as a means of regulating gene expression. Yet, RNA degradation machinery has been studied extensively in only a few eukaryotic organisms, including Saccharomyces cerevisiae and humans. Giardia lamblia is a parasitic protist with unusual genomic traits: it is binucleated and tetraploid, has a very compact genome, displays a theme of genomic minimalism with cellular machinery commonly comprised of a reduced number of protein components, and has a remarkably large population of long, stable, noncoding, antisense RNAs. Here we use in silico approaches to investigate the major RNA degradation machinery in Giardia lamblia and compare it to a broad array of other parasitic protists. We have found key constituents of the deadenylation and decapping machinery and of the 5'-3' RNA degradation pathway. We have similarly found that all of the major 3'-5' RNA degradation pathways are present in Giardia, including both exosome-dependent and exosome-independent machinery. However, we observe significant loss of RNA degradation machinery genes that will result in important differences in the protein composition, and potentially functionality, of the various RNA degradation pathways. This is most apparent in the exosome, the central mediator of 3'-5' degradation, which apparently contains an altered core configuration in both Giardia and Plasmodium, with only four, instead of the canonical six, distinct subunits. Additionally the exosome in Giardia is missing both the Rrp6, Nab3, and Nrd1 proteins, known to be key regulators of noncoding transcript stability in other cells. These findings suggest that although the full complement of the major RNA degradation mechanisms were present - and likely functional - early in eukaryotic evolution, the composition and function of the complexes is more variable than previously

Assessment of exposure to manganese in welding operations during the assembly of heavy excavation machinery accessories.

Science.gov (United States)

Smargiassi, A; Baldwin, M; Savard, S; Kennedy, G; Mergler, D; Zayed, J

2000-10-01

Welder exposure to metals in various industrial sectors is poorly characterized. We had the opportunity to carry out an exploratory study to characterize manganese exposure in welding operations in a recently established Quebec factory that assembled accessories for heavy excavation machinery. Ten workers were sampled for total manganese for at least two consecutive days out of three followed by two consecutive days for respirable manganese (with a size selective sampler with a median cut-off of 4 microns), during a typical week in the summer of 1998. Parts being welded were characterized as large or small. Small parts were those being welded on tables during subassembly. Workers were divided into two groups according to the parts they were welding. Seventy-eight percent of the total manganese exposure levels of welding operations during the assembly of large accessories of heavy excavation machinery exceeded the manganese American Conference of Governmental Industrial Hygienists (ACGIH) threshold limit value (TLV) of 0.20 mg/m3 (GM 0.24 mg/m3, n = 14) while none exceeded the TLV during the assembly of small pieces (GM 0.06 mg/m3, n = 8). Welding operations during the assembly of large heavy excavation machinery accessories may pose a significant health hazard. Considering the importance of task-related variables affecting exposure among workers, further studies are needed to better characterize exposure determinants of welding operations during the assembly of heavy excavation machinery accessories.

Monitoring of vibrating machinery using artificial neural networks

International Nuclear Information System (INIS)

Alguindigue, I.E.; Loskiewicz-Buczak, A.

1991-01-01

The primary source of vibration in complex engineering systems is rotating machinery. Vibration signatures collected from these components render valuable information about the operational state of the system and may be used to perform diagnostics. For example, the low frequency domain contains information about unbalance, misalignment, instability in journal bearing and mechanical looseness; analysis of the medium frequency range can render information about faults in meshing gear teeth; while the high frequency domain will contain information about incipient faults in rolling-element bearings. Trend analysis may be performed by comparing the vibration spectrum for each machine with a reference spectrum and evaluating the vibration magnitude changes at different frequencies. This form of analysis for diagnostics is often performed by maintenance personnel monitoring and recording transducer signals and analyzing the signals to identify the operating condition of the machine. With the advent of portable fast Fourier transform (FFT) analyzers and ''laptop'' computers, it is possible to collect and analyze vibration data an site and detect incipient failures several weeks or months before repair is necessary. It is often possible to estimate the remaining life of certain systems once a fault has been detected. RMS velocity, acceleration, displacements, peak value, and crest factor readings can be collected from vibration sensors. To exploit all the information embedded in these signals, a robust and advanced analysis technique is required. Our goal is to design a diagnostic system using neural network technology, a system such as this would automate the interpretation of vibration data coming from plant-wide machinery and permit efficient on-line monitoring of these components

Air formaldehyde and solvent concentrations during surface coating with acid-curing lacquers and paints in the woodworking and furniture industry.

Science.gov (United States)

Thorud, Syvert; Gjolstad, Merete; Ellingsen, Dag G; Molander, Paal

2005-06-01

An investigation of contemporary exposure to formaldehyde and organic solvents has been carried out during surface coating with acid-curing lacquers and paints in the Norwegian woodworking and furniture industry over a period of 3 years. The investigation covered 27 factories of different sizes and with different types of production, and totally 557 parallel formaldehyde and solvent samples were collected. The formaldehyde concentration (geometric mean) was 0.15 ppm (range 0.01-1.48 ppm) with about 10% of the samples exceeding the Norwegian occupational exposure limit of 0.5 ppm. The solvent concentration as additive effect (geometric mean) was 0.13 (range 0.0004-5.08) and about 5% of the samples exceeded the Norwegian occupational exposure limit. The most frequently occurring solvents from acid-curing lacquers were n-butyl acetate, ethanol, ethyl acetate and 1-butanol, which were found in 88-98% of the samples. Toluene, n-butyl acetate and 1-butanol were the only solvents with maximum concentrations exceeding their respective occupational exposure limits. Curtain painting machine operators were exposed to the highest concentrations of both formaldehyde (geometric mean 0.51 ppm, range 0.08-1.48 ppm) and organic solvents (additive effect, geometric mean 1.18, range 0.02-5.08). Other painting application work tasks such as automatic and manual spray-painting, manual painting and dip painting, showed on average considerably lower concentrations of both formaldehyde (geometric means 0.07-0.16 ppm) and organic solvents (additive effect, geometric mean 0.02-0.18). Non-painting work tasks also displayed moderate concentrations of formaldehyde (geometric means 0.11-0.17 ppm) and organic solvents (additive effect, geometric mean 0.04-0.07).

Conceptual design and analysis of roads and road construction machinery for initial lunar base operations

Science.gov (United States)

Sines, Jeffrey L.; Banks, Joel; Efatpenah, Keyanoush

1990-01-01

Recent developments have made it possible for scientists and engineers to consider returning to the Moon to build a manned lunar base. The base can be used to conduct scientific research, develop new space technology, and utilize the natural resources of the Moon. Areas of the base will be separated, connected by a system of roads that reduce the power requirements of vehicles traveling on them. Feasible road types for the lunar surface were analyzed and a road construction system was designed for initial lunar base operations. A model was also constructed to show the system configuration and key operating features. The alternate designs for the lunar road construction system were developed in four stages: analyze and select a road type; determine operations and machinery needed to produce the road; develop machinery configurations; and develop alternates for several machine components. A compacted lunar soil road was selected for initial lunar base operations. The only machinery required to produce this road were a grader and a compactor. The road construction system consists of a main drive unit which is used for propulsion, a detachable grader assembly, and a towed compactor.

The Antioxidant Machinery of Young and Senescent Human Umbilical Vein Endothelial Cells and Their Microvesicles

Directory of Open Access Journals (Sweden)

Guillermo Bodega

2017-01-01

Full Text Available We examine the antioxidant role of young and senescent human umbilical vein endothelial cells (HUVECs and their microvesicles (MVs. Proteomic and Western blot studies have shown young HUVECs to have a complete and well-developed antioxidant system. Their MVs also contain antioxidant molecules, though of a smaller and more specific range, specialized in the degradation of hydrogen peroxide and the superoxide anion via the thioredoxin-peroxiredoxin system. Senescence was shown to be associated with a large increase in the size of the antioxidant machinery in both HUVECs and their MVs. These responses might help HUVECs and their MVs deal with the more oxidising conditions found in older cells. Functional analysis confirmed the antioxidant machinery of the MVs to be active and to increase in size with senescence. No glutathione or nonpeptide antioxidant (ascorbic acid and vitamin E activity was detected in the MVs. Endothelial cells and MVs seem to adapt to higher ROS concentrations in senescence by increasing their antioxidant machinery, although this is not enough to recover completely from the senescence-induced ROS increase. Moreover, MVs could be involved in the regulation of the blood plasma redox status by functioning as ROS scavengers.

A sequential fuzzy diagnosis method for rotating machinery using ant colony optimization and possibility theory

Energy Technology Data Exchange (ETDEWEB)

Sun, Hao; Ping, Xueliang; Cao, Yi; Lie, Ke [Jiangnan University, Wuxi (China); Chen, Peng [Mie University, Mie (Japan); Wang, Huaqing [Beijing University, Beijing (China)

2014-04-15

This study proposes a novel intelligent fault diagnosis method for rotating machinery using ant colony optimization (ACO) and possibility theory. The non-dimensional symptom parameters (NSPs) in the frequency domain are defined to reflect the features of the vibration signals measured in each state. A sensitive evaluation method for selecting good symptom parameters using principal component analysis (PCA) is proposed for detecting and distinguishing faults in rotating machinery. By using ACO clustering algorithm, the synthesizing symptom parameters (SSP) for condition diagnosis are obtained. A fuzzy diagnosis method using sequential inference and possibility theory is also proposed, by which the conditions of the machinery can be identified sequentially. Lastly, the proposed method is compared with a conventional neural networks (NN) method. Practical examples of diagnosis for a V-belt driving equipment used in a centrifugal fan are provided to verify the effectiveness of the proposed method. The results verify that the faults that often occur in V-belt driving equipment, such as a pulley defect state, a belt defect state and a belt looseness state, are effectively identified by the proposed method, while these faults are difficult to detect using conventional NN.

Industrial policy and technology diffusion : evidence from paper making machinery in Indonesia

NARCIS (Netherlands)

Dijk, van M.; Szirmai, A.

2006-01-01

In this paper, we analyze the diffusion and adoption of paper making machinery in the Indonesian pulp and paper industry, from 1923 till 2000. We develop a machine level index of technological sophistication (mach), which measures the technological distance of each paper machine to the world

New Fault Recognition Method for Rotary Machinery Based on Information Entropy and a Probabilistic Neural Network.

Science.gov (United States)

Jiang, Quansheng; Shen, Yehu; Li, Hua; Xu, Fengyu

2018-01-24

Feature recognition and fault diagnosis plays an important role in equipment safety and stable operation of rotating machinery. In order to cope with the complexity problem of the vibration signal of rotating machinery, a feature fusion model based on information entropy and probabilistic neural network is proposed in this paper. The new method first uses information entropy theory to extract three kinds of characteristics entropy in vibration signals, namely, singular spectrum entropy, power spectrum entropy, and approximate entropy. Then the feature fusion model is constructed to classify and diagnose the fault signals. The proposed approach can combine comprehensive information from different aspects and is more sensitive to the fault features. The experimental results on simulated fault signals verified better performances of our proposed approach. In real two-span rotor data, the fault detection accuracy of the new method is more than 10% higher compared with the methods using three kinds of information entropy separately. The new approach is proved to be an effective fault recognition method for rotating machinery.

A Diagnosis Method for Rotation Machinery Faults Based on Dimensionless Indexes Combined with K-Nearest Neighbor Algorithm

Directory of Open Access Journals (Sweden)

Jianbin Xiong

2015-01-01

Full Text Available It is difficult to well distinguish the dimensionless indexes between normal petrochemical rotating machinery equipment and those with complex faults. When the conflict of evidence is too big, it will result in uncertainty of diagnosis. This paper presents a diagnosis method for rotation machinery fault based on dimensionless indexes combined with K-nearest neighbor (KNN algorithm. This method uses a KNN algorithm and an evidence fusion theoretical formula to process fuzzy data, incomplete data, and accurate data. This method can transfer the signals from the petrochemical rotating machinery sensors to the reliability manners using dimensionless indexes and KNN algorithm. The input information is further integrated by an evidence synthesis formula to get the final data. The type of fault will be decided based on these data. The experimental results show that the proposed method can integrate data to provide a more reliable and reasonable result, thereby reducing the decision risk.

Industrial policy and technology diffusion : evidence from paper making machinery in Indonesia

NARCIS (Netherlands)

Dijk, van M.; Szirmai, A.

2005-01-01

In this paper, we analyse the diffusion and adoption of paper making machinery in the Indonesian pulp and paper industry, from 1923 till 2000. The two main questions in this paper are: (1) What patterns of technology diffusion can be discerned in the Indonesian paper manufacturing sector? (2) What

46 CFR 167.15-25 - Inspection standards for hulls, boilers and machinery.

Science.gov (United States)

2010-10-01

... SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS Inspections § 167.15-25 Inspection standards for hulls, boilers and... 46 Shipping 7 2010-10-01 2010-10-01 false Inspection standards for hulls, boilers and machinery... inspection. These rules may be purchased from the American Bureau of Shipping (ABS), ABS Plaza, 16855...

Transportation of part supply improvement in agricultural machinery assembly plant

Science.gov (United States)

Saysaman, Anusit; Chutima, Parames

2018-02-01

This research focused on the problem caused by the transportation of part supply in agricultural machinery assembly plant in Thailand, which is one of the processes that are critical to the whole production process. If poorly managed, it will affect transportation of part supply, the emergence of sink cost, quality problems, and the ability to respond to the needs of the customers in time. Since the competition in the agricultural machinery market is more intense, the efficiency of part transportation process has to be improved. In this study, the process of transporting parts of the plant was studied and it was found that the efficiency of the process of transporting parts from the sub assembly line to its main assembly line was 83%. The approach to the performance improvement is done by using the Lean tool to limit wastes based on the ECRS principle and applying pull production system by changing the transportation method to operate as milkrun for transportation of parts to synchronize with the part demands of the main assembly line. After the transportation of parts from sub-assembly line to the main assembly line was improved, the efficiency raised to 98% and transportation process cost was saved to 540,000 Baht per year.

Assessment of the most significant causes of transportation and machinery accidents on collieries

CSIR Research Space (South Africa)

Oberholzer, JW

1995-08-01

Full Text Available The purpose of this study is to identify those areas, classified according to the SAMRASS data base system under the codes relating to underground transport and machinery type accidents that give cause to the greatest amount of accidents...

The adaptive response of lichens to mercury exposure involves changes in the photosynthetic machinery

International Nuclear Information System (INIS)

Nicolardi, Valentina; Cai, Giampiero; Parrotta, Luigi; Puglia, Michele; Bianchi, Laura; Bini, Luca; Gaggi, Carlo

2012-01-01

Lichens are an excellent model to study the bioaccumulation of heavy metals but limited information is available on the molecular mechanisms occurring during bioaccumulation. We investigated the changes of the lichen proteome during exposure to constant concentrations of mercury. We found that most of changes involves proteins of the photosynthetic pathway, such as the chloroplastic photosystem I reaction center subunit II, the oxygen-evolving protein and the chloroplastic ATP synthase β-subunit. This suggests that photosynthesis is a target of the toxic effects of mercury. These findings are also supported by changes in the content of photosynthetic pigments (chlorophyll a and b, and β-carotene). Alterations to the photosynthetic machinery also reflect on the structure of thylakoid membranes of algal cells. Response of lichens to mercury also involves stress-related proteins (such as Hsp70) but not cytoskeletal proteins. Results suggest that lichens adapt to mercury exposure by changing the metabolic production of energy. - Highlights: ► Lichens exposed to Hg° vapors accumulate this metal irreversibly. ► Hg° interferes with physiological processes of the epiphytic lichen Evernia prunastri. ► Hg° promotes changes in the concentration of photosynthetic pigments. ► Hg° treatment causes changes in the ultrastructure of the photobiont plastids. ► Hg° induces changes in the protein machinery involved in the photosynthesis pathway. - Mercury affects the photosynthetic protein machinery of lichens.

46 CFR 62.50-30 - Additional requirements for periodically unattended machinery plants.

Science.gov (United States)

2010-10-01

... include machinery plants and spaces that are automated to the degree that they are self-regulating and... engineers' accommodations. Other than fire or flooding alarms, this may be accomplished by summarized visual... in § 111.103 of this chapter; and (7) Voice communications with the bridge. (i) Oil leakage. Leakages...

Targeting apoptotic machinery as approach for anticancer therapy: Smac mimetics as anticancer agents

Directory of Open Access Journals (Sweden)

Nevine M.Y. Elsayed

2015-06-01

Full Text Available Apoptosis is a chief regulator of cellular homeostasis. Impairment of apoptotic machinery is a main characteristic of several diseases such as cancer, where the evasion of apoptosis is a cardinal hallmark of cancer. Apoptosis is regulated by contribution of pro- and anti- apoptotic proteins, where caspases are the main executioners of the apoptotic machinery. IAP (inhibitors of apoptosis proteins is a family of endogenous inhibitors of apoptosis, which perform their function through interference with the function of caspases. Smac (second mitochondria-derived activator of caspases is endogenous inhibitor of IAPs, thus it is one of the major proapoptotic endogenous proteins. Thus, the development of Smac mimetics has evolved as an approach for anticancer therapy. Several Smac mimetic agents have been introduced to clinical trial such as birinapanet 12. Herein, the history of development of Smac mimetics along with the recent development in this field is briefly discussed.

4th International Conference on Condition Monitoring of Machinery in Non-Stationary Operations

CERN Document Server

Zimroz, Radoslaw; Bartelmus, Walter; Haddar, Mohamed

2016-01-01

The book provides readers with a snapshot of recent research and technological trends in the field of condition monitoring of machinery working under a broad range of operating conditions. Each chapter, accepted after a rigorous peer-review process, reports on an original piece of work presented and discussed at the 4th International Conference on Condition Monitoring of Machinery in Non-stationary Operations, CMMNO 2014, held on December 15-16, 2014, in Lyon, France. The contributions have been grouped into three different sections according to the main subfield (signal processing, data mining, or condition monitoring techniques) they are related to. The book includes both theoretical developments as well as a number of industrial case studies, in different areas including, but not limited to: noise and vibration; vibro-acoustic diagnosis; signal processing techniques; diagnostic data analysis; instantaneous speed identification; monitoring and diagnostic systems; and dynamic and fault modeling. This book no...

Research on a Rotating Machinery Fault Prognosis Method Using Three-Dimensional Spatial Representations

Directory of Open Access Journals (Sweden)

Xiaoni Dong

2016-01-01

Full Text Available Process models and parameters are two critical steps for fault prognosis in the operation of rotating machinery. Due to the requirement for a short and rapid response, it is important to study robust sensor data representation schemes. However, the conventional holospectrum defined by one-dimensional or two-dimensional methods does not sufficiently present this information in both the frequency and time domains. To supply a complete holospectrum model, a new three-dimensional spatial representation method is proposed. This method integrates improved three-dimensional (3D holospectra and 3D filtered orbits, leading to the integration of radial and axial vibration features in one bearing section. The results from simulation and experimental analysis on a complex compressor show that the proposed method can present the real operational status and clearly reveal early faults, thus demonstrating great potential for condition-based maintenance prediction in industrial machinery.

Developing an Online Learning Media Using Smartphone for the Electrical Machinery Course

Science.gov (United States)

Muchlas

2018-01-01

This research is aimed to prepare a desktop-based learning media that can be used to support an online lab activities using android smartphones in Electrical Machinery Course at the Department of Electrical Engineering for the undergraduate level. This work uses a conceptual development model which integrates some sub systems of internet…

Repetitive stimulation of autophagy-lysosome machinery by intermittent fasting preconditions the myocardium to ischemia-reperfusion injury.

Science.gov (United States)

Godar, Rebecca J; Ma, Xiucui; Liu, Haiyan; Murphy, John T; Weinheimer, Carla J; Kovacs, Attila; Crosby, Seth D; Saftig, Paul; Diwan, Abhinav

2015-01-01

Autophagy, a lysosomal degradative pathway, is potently stimulated in the myocardium by fasting and is essential for maintaining cardiac function during prolonged starvation. We tested the hypothesis that intermittent fasting protects against myocardial ischemia-reperfusion injury via transcriptional stimulation of the autophagy-lysosome machinery. Adult C57BL/6 mice subjected to 24-h periods of fasting, every other day, for 6 wk were protected from in-vivo ischemia-reperfusion injury on a fed day, with marked reduction in infarct size in both sexes as compared with nonfasted controls. This protection was lost in mice heterozygous null for Lamp2 (coding for lysosomal-associated membrane protein 2), which demonstrate impaired autophagy in response to fasting with accumulation of autophagosomes and SQSTM1, an autophagy substrate, in the heart. In lamp2 null mice, intermittent fasting provoked progressive left ventricular dilation, systolic dysfunction and hypertrophy; worsening cardiomyocyte autophagosome accumulation and lack of protection to ischemia-reperfusion injury, suggesting that intact autophagy-lysosome machinery is essential for myocardial homeostasis during intermittent fasting and consequent ischemic cardioprotection. Fasting and refeeding cycles resulted in transcriptional induction followed by downregulation of autophagy-lysosome genes in the myocardium. This was coupled with fasting-induced nuclear translocation of TFEB (transcription factor EB), a master regulator of autophagy-lysosome machinery; followed by rapid decline in nuclear TFEB levels with refeeding. Endogenous TFEB was essential for attenuation of hypoxia-reoxygenation-induced cell death by repetitive starvation, in neonatal rat cardiomyocytes, in-vitro. Taken together, these data suggest that TFEB-mediated transcriptional priming of the autophagy-lysosome machinery mediates the beneficial effects of fasting-induced autophagy in myocardial ischemia-reperfusion injury.

Repetitive stimulation of autophagy-lysosome machinery by intermittent fasting preconditions the myocardium to ischemia-reperfusion injury

Science.gov (United States)

Godar, Rebecca J; Ma, Xiucui; Liu, Haiyan; Murphy, John T; Weinheimer, Carla J; Kovacs, Attila; Crosby, Seth D; Saftig, Paul; Diwan, Abhinav

2015-01-01

Autophagy, a lysosomal degradative pathway, is potently stimulated in the myocardium by fasting and is essential for maintaining cardiac function during prolonged starvation. We tested the hypothesis that intermittent fasting protects against myocardial ischemia-reperfusion injury via transcriptional stimulation of the autophagy-lysosome machinery. Adult C57BL/6 mice subjected to 24-h periods of fasting, every other day, for 6 wk were protected from in-vivo ischemia-reperfusion injury on a fed day, with marked reduction in infarct size in both sexes as compared with nonfasted controls. This protection was lost in mice heterozygous null for Lamp2 (coding for lysosomal-associated membrane protein 2), which demonstrate impaired autophagy in response to fasting with accumulation of autophagosomes and SQSTM1, an autophagy substrate, in the heart. In lamp2 null mice, intermittent fasting provoked progressive left ventricular dilation, systolic dysfunction and hypertrophy; worsening cardiomyocyte autophagosome accumulation and lack of protection to ischemia-reperfusion injury, suggesting that intact autophagy-lysosome machinery is essential for myocardial homeostasis during intermittent fasting and consequent ischemic cardioprotection. Fasting and refeeding cycles resulted in transcriptional induction followed by downregulation of autophagy-lysosome genes in the myocardium. This was coupled with fasting-induced nuclear translocation of TFEB (transcription factor EB), a master regulator of autophagy-lysosome machinery; followed by rapid decline in nuclear TFEB levels with refeeding. Endogenous TFEB was essential for attenuation of hypoxia-reoxygenation-induced cell death by repetitive starvation, in neonatal rat cardiomyocytes, in-vitro. Taken together, these data suggest that TFEB-mediated transcriptional priming of the autophagy-lysosome machinery mediates the beneficial effects of fasting-induced autophagy in myocardial ischemia-reperfusion injury. PMID:26103523

Diagnostic suite neuro-fuzzy in an advanced alarm monitoring and predictive diagnostic system for rotating machinery

International Nuclear Information System (INIS)

Geruzzi, P.

1999-01-01

The 'Foxboro SCADA', former 'Automation Systems Division' of Nuovo Pignone, at the end of eighty years, has been involved in the development of a flexible and powerful Diagnostic System for Rotating Machinery designed and manufactured in other divisions of the Company. This system amalgamates, in a single computer, all the functionality nowadays necessary to correctly manage locally and remotely the Evolutionary Maintenance of rotating machines as well as the relevant plants. It's specially designed to plan preventive and emergency maintenance procedures and to help the maintenance staff/service in preventing the occurrence of failures or severe damage to complete turbo-machinery plant including turbine, compressor and other machines. The system is designed to supervise and to analyze the operating state of one or more turbo- machinery units such as turbo-compressors, turbo-generators and turbo-pumps giving an effective support to plan preventive and breakdown maintenance monitoring the performances of each turbo-group's element and analyzing a large number of thermodynamic and mechanical parameters related to high pressure turbines, low pressure turbines, combustion chambers, axial compressors and load (compressors, generators, and pumps). A brief presentation of the system is provided (author) (ml)

Research Using Virtual Reality: Mobile Machinery Safety in the 21st Century

Directory of Open Access Journals (Sweden)

Giselle P. Delgado

2013-04-01

Full Text Available Whole-body vibration is a significant health risk for between 4% and 7% of the work force in North America. In addition, many factors compound the health risks of heavy machinery operators. For example, twisted trunk and neck postures stiffen the spine and increase the transmission of vibration to the head. Similarly, workers adopt awkward postures in order to gain appropriate lines of sight for machine operations. Although the relative contribution of these various issues can be evaluated in field studies and models, we propose that virtual reality is a powerful medium for investigating issues related to health and safety in mining machine operators. We have collected field data of posture and vibration, as well as visual environment, for a forklift operating in a warehouse. This paper describes the process and outcome of this field data collection, and provides a discussion on the next steps to develop and test the virtual reality model to enable laboratory testing. Our ongoing studies will evaluate the interplay between posture and vibration under conditions replicating routine heavy machinery operations, such as underground mining.

49 CFR 1242.42 - Administration, repair and maintenance, machinery repair, equipment damaged, dismantling retired...

Science.gov (United States)

2010-10-01

... repair, equipment damaged, dismantling retired property, fringe benefits, other casualties and insurance, lease rentals, joint facility rents, other rents, depreciation, joint facility, repairs billed to others... maintenance, machinery repair, equipment damaged, dismantling retired property, fringe benefits, other...

Folding machineries displayed on a cation-exchanger for the concerted refolding of cysteine- or proline-rich proteins

Directory of Open Access Journals (Sweden)

Lee Dae-Hee

2009-03-01

Full Text Available Abstract Background Escherichia coli has been most widely used for the production of valuable recombinant proteins. However, over-production of heterologous proteins in E. coli frequently leads to their misfolding and aggregation yielding inclusion bodies. Previous attempts to refold the inclusion bodies into bioactive forms usually result in poor recovery and account for the major cost in industrial production of desired proteins from recombinant E. coli. Here, we describe the successful use of the immobilized folding machineries for in vitro refolding with the examples of high yield refolding of a ribonuclease A (RNase A and cyclohexanone monooxygenase (CHMO. Results We have generated refolding-facilitating media immobilized with three folding machineries, mini-chaperone (a monomeric apical domain consisting of residues 191–345 of GroEL and two foldases (DsbA and human peptidyl-prolyl cis-trans isomerase by mimicking oxidative refolding chromatography. For efficient and simple purification and immobilization simultaneously, folding machineries were fused with the positively-charged consecutive 10-arginine tag at their C-terminal. The immobilized folding machineries were fully functional when assayed in a batch mode. When the refolding-facilitating matrices were applied to the refolding of denatured and reduced RNase A and CHMO, both of which contain many cysteine and proline residues, RNase A and CHMO were recovered in 73% and 53% yield of soluble protein with full enzyme activity, respectively. Conclusion The refolding-facilitating media presented here could be a cost-efficient platform and should be applicable to refold a wide range of E. coli inclusion bodies in high yield with biological function.

Necrosome core machinery: MLKL.

Science.gov (United States)

Zhang, Jing; Yang, Yu; He, Wenyan; Sun, Liming

2016-06-01

In the study of regulated cell death, the rapidly expanding field of regulated necrosis, in particular necroptosis, has been drawing much attention. The signaling of necroptosis represents a sophisticated form of a death pathway. Anti-caspase mechanisms (e.g., using inhibitors of caspases, or genetic ablation of caspase-8) switch cell fate from apoptosis to necroptosis. The initial extracellular death signals regulate RIP1 and RIP3 kinase activation. The RIP3-associated death complex assembly is necessary and sufficient to initiate necroptosis. MLKL was initially identified as an essential mediator of RIP1/RIP3 kinase-initiated necroptosis. Recent studies on the signal transduction using chemical tools and biomarkers support the idea that MLKL is able to make more functional sense for the core machinery of the necroptosis death complex, called the necrosome, to connect to the necroptosis execution. The experimental data available now have pointed that the activated MLKL forms membrane-disrupting pores causing membrane leakage, which extends the prototypical concept of morphological and biochemical events following necroptosis happening in vivo. The key role of MLKL in necroptosis signaling thus sheds light on the logic underlying this unique "membrane-explosive" cell death pathway. In this review, we provide the general concepts and strategies that underlie signal transduction of this form of cell death, and then focus specifically on the role of MLKL in necroptosis.

Reconstitution of Biosynthetic Machinery for the Synthesis of the Highly Elaborated Indole Diterpene Penitrem

DEFF Research Database (Denmark)

Liu, Chengwei; Tagami, Koichi; Minami, Atsushi

2015-01-01

KULNJ). Importantly, without conventional gene disruption, reconstitution of the biosynthetic machinery provided sufficient data to determine the pathway. It was thus demonstrated that the Aspergillus oryzae reconstitution system is a powerful method for studying the biosynthesis of complex natural products....

OPTIMIZATION MODEL FOR VEHICLE ROUTING AND EQUIPMENT REPLACEMENT IN FARM MACHINERY

OpenAIRE

Grano, Carolina; Abensur, Eder

2017-01-01

ABSTRACT: An equipment replacement decision takes into account economic engineering models based on discounted cash flow (DCF) such as the Annual Equivalent Cost (AEC). Despite a large number of researches on industrial assets replacement, there is a lack of studies applied to farm goods. This study aimed at assessing an alternative model for economic decision analysis on farm machinery replacement, with no restrictions on the number of replacements and assessed goods during a defined timelin...

Triple Recycling Channel Strategies for Remanufacturing of Construction Machinery in a Retailer-Dominated Closed-Loop Supply Chain

Directory of Open Access Journals (Sweden)

Min Huang

2017-12-01

Full Text Available Firms engaged in remanufacturing activities generally adopt more than one recycling channel to collect more used products and gain more profits. This paper explores the optimal strategies for a retailer-dominated closed-loop supply chain (CLSC with a triple recycling channel in the construction machinery remanufacturing context. In this special system, the retailer is the leader and authorized by the original equipment manufacturer (OEM to remanufacture. Moreover, the OEM, the retailer, and the secondary market all take part in the used products collection activities. Considering the differentiation of the OEM, the retailer, and the secondary market in collecting the used construction machinery, a mathematical model of the CLSC system based on reasonable assumptions is built, the closed-form optimal pricing decisions are derived, and the optimal collection efforts allocation strategies are explored within the framework of the game theory. In addition, the impacts of the reverse logistics cost coefficient, the competing coefficient, and the buy-back price coefficient on the supply chain performance are elaborately analyzed. These achievements provide decision makers with managerial insights and offer efficient guidelines for the construction machinery remanufacturing firms to solve similar puzzles.

77 FR 64462 - Conference on International Trade Issues Facing U.S. Machinery Manufacturing Industries

Science.gov (United States)

2012-10-22

... manufacturing is one of the largest and most competitive sectors of the American manufacturing economy. The... billion in 2011. The economic impact of machinery manufacturing extends throughout the American economy... service industries. Process control and other automation technologies enable end-users to maximize the...

Legal regulations of restrictions of air pollution made by non-road mobile machinery-the case study for Europe: a review.

Science.gov (United States)

Waluś, Konrad J; Warguła, Łukasz; Krawiec, Piotr; Adamiec, Jarosław M

2018-02-01

The high awareness of intensification and frequency of smog phenomenon all over the world in XXI age makes for detailed analyses of the reasons of its formation and prevention. The governments of the developed countries and conscious of real hazards, including many European countries, aim to restrict the emission of harmful gases. In literature, we can find the discussions on the influence of this phenomenon on the health and life of inhabitants of contaminated areas. Some elaborations of prognostic models, descriptions of pollution sources, the manner of their restriction, and the analysis of causal-consecutive correlation are also popular. The influence of pollutions resulting from the operation of vehicles, planes, and the industry are well described. However, every machine and device which is driven with a combustion engine has the effect on the general level of anthropogenic pollutions. These drives are subject of different regulations limiting their emission for service conditions and applications. One of the groups of such machines described in European and American regulations is non-road mobile machinery. The aim of this paper is the presentation of the problem of weak analysis and application of engineering and technological tools for machinery drive emission, despite of many publications on hazards and problems of emission. These machines have the influence on both the increase of global contamination and the machine users. The regulations of the European Union take into consideration the generated hazards and restrict the emission of machine exhaust gases by approval tests-these regulations are continually improved, and the effects of these works are new emission limits in 2019. However, these activities seem to be liberal as opposed to limits of the emission for passenger and goods vehicles where the technological development of the construction is greater and the regulations are the most rigorous. During the analysis of the development of non

The Machinery Of Climate Anti-Science, Its Efforts Against Education, Top To Bottom

Science.gov (United States)

Mashey, J. R.

2014-12-01

"There's always one every year" a fine local science teacher said after once again being hassled by a vocal parent for teaching appropriate climate science in school. How does that happen? The machinery of climate anti-science starts from the top with funders working through a maze of money paths, think tanks and front groups, employing spokespeople who can be portrayed as experts. While much of the money flows are still dark, some have been exposed over the last few years, and the effects finally filter down to the state and local levels of education. Among others, the Heartland Institute has a long history of trying to inject anti-science into K-12 and college education, having sent books, DVDs or brochures to teachers or school boards, as well as monthly newsletters to state legislators. Such are aimed at the top of the state or local organizations that affect education. For a vocal subset of the citizenry, a constant flow of misinformation from books, blogs, newsletters and some newspapers and magazines stirs action such as writing letters to editors, complaining to schools, calling on pseudo-experts and demanding equal time for pseudoscience. As the teacher said, it only takes one person to cause trouble at the local level. After a brief review of the overall machinery, this focuses on examples of anti-education tactics seen already, with some brief advice for climate scientists and educators who need to understand the machinery that supports such tactics. Some earlier history is included in http://www.desmogblog.com/2012/10/23/fakery-2-more-funny-finances-free-tax, but other attempts have surfaced in last few years, including state-wide efforts to reject Common Core Educational standard to avoid teaching climate science. Fortunately, school boards sometimes respond quite well, including one just recently in Pennsylvania.

Human Machine Interaction by Simulation of Dynamics of Construction Machinery

DEFF Research Database (Denmark)

Langer, Thomas Heegaard

-body vibration exposure was more than 20 percent and at the same time the fuel consumption was reduced significant. Training of operators is hence beneficial for both employees and employers of the construction industry. The whole-body vibration exposure on operators of dump trucks are dominated by off-road......This industrial Ph.D. project concerns whole-body vibrations in human operated construction machinery. The emissions of these vibrations is closely related to the subjective experience of comfort and in some cases these vibrations can occur in a level which can cause the operator back disorders...

WAVELETS AND PRINCIPAL COMPONENT ANALYSIS METHOD FOR VIBRATION MONITORING OF ROTATING MACHINERY

OpenAIRE

Bendjama, Hocine; S. Boucherit, Mohamad

2017-01-01

Fault diagnosis is playing today a crucial role in industrial systems. To improve reliability, safety and efficiency advanced monitoring methods have become increasingly important for many systems. The vibration analysis method is essential in improving condition monitoring and fault diagnosis of rotating machinery. Effective utilization of vibration signals depends upon effectiveness of applied signal processing techniques. In this paper, fault diagnosis is performed using a com...

78 FR 31897 - Agency Information Collection Activities; Proposed Collection; Comment Request; CPSC Table Saw...

Science.gov (United States)

2013-05-28

... Commission. ACTION: Notice. SUMMARY: The Consumer Product Safety Commission (CPSC or Commission) is... respondents, advertisements will be placed on popular Web sites, in woodworking magazines, and posted in woodworking guilds with their cooperation. Respondents will have the option to go through a screening process...

Physical and Mechanical Properties of Palm Oil Frond and Stem Bunch for Developing Pruner and Harvester Machinery Design

Directory of Open Access Journals (Sweden)

Yazid Ismi Intara

2013-06-01

Full Text Available A development of oil palm pruner and harvester machinery design implemented in the field still faces a problem due to the lack of effective and efficient design which is need to be solved. It was noted that in order to develop the design, an early data and information of physical and mechanical properties of palm oil frond and stem fruits is critically important. The objective of the research was to obtain the physical and mechanical properties of palm oil frond and stem in order to develop the design of pruner and harvester machinery. The result showed that tool machinery was been advantageous by the physical properties of the plant i.e. the total weight of frond and leaf which enable to support the cutting process. The average of total weight of frond and leaf was 16.8 kg. The diagonal cutting trajectory was been more advantageous because of total weight and frond shape toward to the different of the plant tissue area. The measurement result shows that cutting curve follows the time required for cutting. The comparison among cutting curve shows differences in cutting thickness or length. In this case, the thickness is linear with cutting time. Besides, those curves show differences at the height which determine the maximum value of tested material cutting resistance. Alternative solution for machinery development design is pruner-harvester for height plant below 6 m and among 6 to 12 m. For below 6 m, pruner-harvester was designed by incorporating motor as power source and cutter-disc as the knife cutter. That condition was relied on that estate which was maintenance intensively commonly used cutter-disc. Pruner-harvester above 6 m and up to 12 m was improved based on manual egrek-designed by adding fresh fruit bunch alley supply glide in order to keep the fruits still in intact form. The consideration was based on affectivity and efficiency. It also considers homogenous ecological of palm oil plant which should be maintained to reduce global

Accessing and operating agricultural machinery: Advancements in assistive technology for users with impaired mobility.

Science.gov (United States)

Ehlers, Shawn G; Field, William E

2018-02-14

This research focused on the advancements made in enabling agricultural workers with impaired mobility to access and operate off-road agricultural machinery. Although not a new concept, technological advancements in remote-controlled lifts, electronic actuators, electric over hydraulic controllers, and various modes of hand controls have advanced significantly, allowing operators with limited mobility to resume a high level of productivity in agricultural-related enterprises. In the United States, approximately 1.7% of the population is living with some form of paralysis or significant mobility impairment. When paired with the 2012 USDA Agriculture Census of 3.2 million farmers, it can be extrapolated that these technologies could impact 54,000 agricultural workers who have encountered disabling injuries or disease, which inhibit their ability to access and operate tractors, combines, and other self-propelled agricultural machines. Advancements in agricultural-specific technologies can allow for many of these individuals to regain the ability to effectively operate machinery once more.

Vibration Signal Forecasting on Rotating Machinery by means of Signal Decomposition and Neurofuzzy Modeling

Directory of Open Access Journals (Sweden)

Daniel Zurita-Millán

2016-01-01

Full Text Available Vibration monitoring plays a key role in the industrial machinery reliability since it allows enhancing the performance of the machinery under supervision through the detection of failure modes. Thus, vibration monitoring schemes that give information regarding future condition, that is, prognosis approaches, are of growing interest for the scientific and industrial communities. This work proposes a vibration signal prognosis methodology, applied to a rotating electromechanical system and its associated kinematic chain. The method combines the adaptability of neurofuzzy modeling with a signal decomposition strategy to model the patterns of the vibrations signal under different fault scenarios. The model tuning is performed by means of Genetic Algorithms along with a correlation based interval selection procedure. The performance and effectiveness of the proposed method are validated experimentally with an electromechanical test bench containing a kinematic chain. The results of the study indicate the suitability of the method for vibration forecasting in complex electromechanical systems and their associated kinematic chains.

78 FR 60844 - Wooden Bedroom Furniture From the People's Republic of China: Partial Rescission of Antidumping...

Science.gov (United States)

2013-10-02

... Furniture Co., Ltd. Dorbest Ltd., Rui Feng Woodwork Co., Ltd. Aka Rui Feng Woodwork (Dongguan) Co., Ltd., Rui Feng Lumber Development Co., Ltd. Aka Rui Feng Lumber Development (Shenzhen) Co., Ltd., Fine... Fung Wooden Factory, Sun Fung Co., Shin Feng Furniture Co., Ltd., Stupendous International Co., Ltd...

CONTRIBUTIONS OF CAD / CAE TECHNOLOGY IN DEVELOPMENT PROJECTS OF AGRICULTURAL MACHINERY AND IMPLEMENTS: A CASE STUDY

Directory of Open Access Journals (Sweden)

Flavio Ricci Martinez

2017-12-01

Full Text Available This work aims to understand the contributions of CAD / CAE technology development projects Agricultural Machinery and Implements. The segment of Agricultural Machinery and Implements part of capital goods, which provides items that will be used to produce goods and services in other sectors and that is strategic in many economically and for innovation and technological diffusion in the country sector. To achieve the proposed objective a case study was conducted at a manufacturer of this type of product (combines, planters, tractors, etc. located in the region of Ribeirão Preto. Among the main contributions can excel found to reduce the time and costs of the project, the largest customer interaction, integration with the staff and the increased ability to test alternative solutions for solving problems.

Domestication of self-splicing introns during eukaryogenesis : the rise of the complex spliceosomal machinery

NARCIS (Netherlands)

Vosseberg, Julian; Snel, Berend

2017-01-01

ᅟ: The spliceosome is a eukaryote-specific complex that is essential for the removal of introns from pre-mRNA. It consists of five small nuclear RNAs (snRNAs) and over a hundred proteins, making it one of the most complex molecular machineries. Most of this complexity has emerged during

Cholinergic Machinery as Relevant Target in Acute Lymphoblastic T Leukemia

Directory of Open Access Journals (Sweden)

Oxana Dobrovinskaya

2016-08-01

Full Text Available Various types of non-neuronal cells, including tumors, are able to produce acetylcholine (ACh, which acts as an autocrine/paracrine growth factor. T lymphocytes represent a key component of the non-neuronal cholinergic system. T cells-derived ACh is involved in a stimulation of their activation and proliferation, and acts as a regulator of immune response. The aim of the present work was to summarize the data about components of cholinergic machinery in T lymphocytes, with an emphasis on the comparison of healthy and leukemic T cells. Cell lines derived from acute lymphoblastic leukemias of T lineage (T-ALL were found to produce a considerably higher amount of ACh than healthy T lumphocytes. Additionally, ACh produced by T-ALL is not efficiently hydrolyzed, because acetylcholinesterase (AChE activity is drastically decreased in these cells. Up-regulation of muscarinic ACh receptors was also demonstrated at expression and functional level, whereas nicotinic ACh receptors seem to play a less important role and not form functional channels in cells derived from T-ALL. We hypothesized that ACh over-produced in T-ALL may act as an autocrine growth factor and play an important role in leukemic clonal expansion through shaping of intracellular Ca2+ signals. We suggest that cholinergic machinery may be attractive targets for new drugs against T-ALL. Specifically, testing of high affinity antagonists of muscarinic ACh receptors as well as antagomiRs, which interfere with miRNAs involved in the suppression of AChE expression, may be the first choice options.

Evaluation Study of VTAE Wood Technics Programs.

Science.gov (United States)

Wisconsin State Board of Vocational, Technical, and Adult Education, Madison.

A survey of former students of the Wisconsin Vocational, Technical, and Adult Education (VTAE) wood technics programs and employers in woodworking industries was conducted during spring of 1985. General objectives were to determine job classifications, types of businesses, and relative importance of tasks or duties in various woodworking-related…

Adaptive Change Detection for Long-Term Machinery Monitoring Using Incremental Sliding-Window

Science.gov (United States)

Wang, Teng; Lu, Guo-Liang; Liu, Jie; Yan, Peng

2017-11-01

Detection of structural changes from an operational process is a major goal in machine condition monitoring. Existing methods for this purpose are mainly based on retrospective analysis, resulting in a large detection delay that limits their usages in real applications. This paper presents a new adaptive real-time change detection algorithm, an extension of the recent research by combining with an incremental sliding-window strategy, to handle the multi-change detection in long-term monitoring of machine operations. In particular, in the framework, Hilbert space embedding of distribution is used to map the original data into the Re-producing Kernel Hilbert Space (RKHS) for change detection; then, a new adaptive threshold strategy can be developed when making change decision, in which a global factor (used to control the coarse-to-fine level of detection) is introduced to replace the fixed value of threshold. Through experiments on a range of real testing data which was collected from an experimental rotating machinery system, the excellent detection performances of the algorithm for engineering applications were demonstrated. Compared with state-of-the-art methods, the proposed algorithm can be more suitable for long-term machinery condition monitoring without any manual re-calibration, thus is promising in modern industries.

Utility machinery vibration monitoring guide: Final report

International Nuclear Information System (INIS)

Moore, T.T.; Thomas, C.C.

1987-08-01

Section I of this guide presents a methodology for developing machinery vibration monitoring programs specifically designed for application within the utility industry. The methodology is designed to enhance a monitoring program and can be used at the outset of program development or as a reference after programs have been started. Section I evaluates all aspects of the monitoring program, including Objectives and Goals, Information Type, Timing and Format, Data Analysis, Data Acquisition, Measurement and Transducer Selection, Personnel and Organization, Program Instrumentation, Program Costs, Program Justification, and Implementation of a Monitoring Program. The methodology is then applied to two host utility plants in Section II, which contains the monitoring programs developed by Gulf States Utilities and Philadelphia Electric Company using this guide. Section III contains the histories of several different types of existing utility monitoring programs. Some of the lessons learned, including the recommendations of these ''mature'' programs for persons starting new programs, are included

Autophagic machinery activated by dengue virus enhances virus replication

International Nuclear Information System (INIS)

Lee, Y.-R.; Lei, H.-Y.; Liu, M.-T.; Wang, J.-R.; Chen, S.-H.; Jiang-Shieh, Y.-F.; Lin, Y.-S.; Yeh, T.-M.; Liu, C.-C.; Liu, H.-S.

2008-01-01

Autophagy is a cellular response against stresses which include the infection of viruses and bacteria. We unravel that Dengue virus-2 (DV2) can trigger autophagic process in various infected cell lines demonstrated by GFP-LC3 dot formation and increased LC3-II formation. Autophagosome formation was also observed under the transmission electron microscope. DV2-induced autophagy further enhances the titers of extracellular and intracellular viruses indicating that autophagy can promote viral replication in the infected cells. Moreover, our data show that ATG5 protein is required to execute DV2-induced autophagy. All together, we are the first to demonstrate that DV can activate autophagic machinery that is favorable for viral replication

Integrated logistics management system for operation of machinery and equipment

Directory of Open Access Journals (Sweden)

Józef Frąś

2014-09-01

Full Text Available Background: The main issue in the operations of machinery and equipment, which is the subject of theoretical and empirical research is to provide high reliability and durability with qualitative post-trade services of machinery and equipment. Quality of service can be achieved through planned maintenance activities supported by computer technology. The article presents the concept of an integrated system of logistics management operation of machinery and equipment, especially special one for stationary transport equipment. At the outset, it emphasized the importance and essence of technological transport and storage systems storage in modern manufacturing enterprise. Then the objective and the method of research have been set. An essential part of deliberations in the article is the concept of integrated logistics management system operation for stationary transport equipment. Authors of this article have presented the results the implementation and operation of the system. The results are presented in a descriptive and graphic form. Methods: The purpose of this article is to present the concept of implementing an integrated logistics management system for operation of stationary transport equipment. It goes through combination of planning, event logging service, warehouse management in the field of spare parts, account and records of the cost of service activities. The paper presents an analysis and evaluation method of brainstorming a new approach to logistics management operation stationary transport equipment. Authors takes into account the specific conditions of use of transport equipment and conduct the service, which have a significant impact on the time and place of cost and service as well. It should be noted that the developed system has been implemented. It was also carried out an assessment of its functionality and efficiency as the new IT tool for logistics management operation. Results and conclusions: The paper presents a new

MODELING ENVIRONMENTAL IMPACT OF MACHINERY SECTORS TO PROMOTE SUSTAINABLE DEVELOPMENT OF THAILAND

OpenAIRE

Pruethsan Sutthichaimethee

2016-01-01

The objective of this research is to propose an indicator to evaluate environmental impacts from the machinery sectors of Thailand, leading to more sustainable consumption and production in this sector of the economy. The factors used to calculate the forward linkage, backward linkage and real benefit included the total environmental costs. The highest total environmental cost was railway equipment need to be resolved immediately because it uses natural resources in carrying capacity, higher ...

Intelligent method for diagnosing structural faults of rotating machinery using ant colony optimization.

Science.gov (United States)

Li, Ke; Chen, Peng

2011-01-01

Structural faults, such as unbalance, misalignment and looseness, etc., often occur in the shafts of rotating machinery. These faults may cause serious machine accidents and lead to great production losses. This paper proposes an intelligent method for diagnosing structural faults of rotating machinery using ant colony optimization (ACO) and relative ratio symptom parameters (RRSPs) in order to detect faults and distinguish fault types at an early stage. New symptom parameters called "relative ratio symptom parameters" are defined for reflecting the features of vibration signals measured in each state. Synthetic detection index (SDI) using statistical theory has also been defined to evaluate the applicability of the RRSPs. The SDI can be used to indicate the fitness of a RRSP for ACO. Lastly, this paper also compares the proposed method with the conventional neural networks (NN) method. Practical examples of fault diagnosis for a centrifugal fan are provided to verify the effectiveness of the proposed method. The verification results show that the structural faults often occurring in the centrifugal fan, such as unbalance, misalignment and looseness states are effectively identified by the proposed method, while these faults are difficult to detect using conventional neural networks.

Investigation and Development of the Thermal Preparation System of the Trailbuilder Machinery Hydraulic Actuator

Science.gov (United States)

Konev, V.; Polovnikov, E.; Krut, O.; Merdanov, Sh; Zakirzakov, G.

2017-07-01

It’s determined that the main part of trailbuilders operated in the North is the technology equipped by the hydraulic actuator. Further development of the northern territories will demand using of various means and ways machinery thermal preparation, and also the machinery of the northern fulfillment. On this basis problems in equipment operation are defined. One of the main is efficiency supplying of a hydraulic actuator. On the basis of the operating conditions’ analysis of trailbuilder hydraulic actuator operation it is determined, that under low negative temperatures the means of thermal preparation are necessary. The existing systems warm up only a hydraulic tank or warming up of the hydro equipment before the machinery operation is carried out under loading with intensive wears. Thus, with the purpose to raise the efficiency of thermal hydraulic actuator, operated far from stationary bases autonomous, energy saving, not expensive in creation and operation systems are necessary. In accordance with the analysis of means and ways of the thermal preparation of the hydraulic actuator and the thermal balance calculations of the (internal) combustion engine the system of the hydraulic actuator heating is offered and is being investigated. It contains a local hydraulic actuator warming up and the system of internal combustion engine heat utilization. Within research operation conditions of the local hydraulic actuator heating are viewed and determined, taking into account constructive changes to the local hydraulic actuator heating. Mathematical modelling of the heat technical process in the modernized hydraulic actuator is considered. As a result temperature changes of the heat-transfer and the hydraulic cylinder in time are determined. To check the theoretical researches and to define dependences on hydraulic actuator warming up, the experimental installation is made. It contains the measuring equipment, a small tank with the heat exchanger of the burnt gases

Comparative study of tool machinery sliding systems; comparison between plane and cylindrical basic shapes

Science.gov (United States)

Glăvan, D. O.; Babanatsas, T.; Babanatis Merce, R. M.; Glăvan, A.

2018-01-01

The paper brings in attention the importance that the sliding system of a tool machinery is having in the final precision of the manufacturing. We are basically comparing two type of slides, one constructed with plane surfaces and the other one with circular cross-sections (as known as cylindrical slides), analysing each solution from the point of view of its technology of manufacturing, of the precision that the particular slides are transferring to the tool machinery, cost of production, etc. Special attention is given to demonstrate theoretical and to confirm by experimental works what is happening with the stress distribution in the case of plane slides and cylindrical slides, both in longitudinal and in cross-over sections. Considering the results obtained for the stress distribution in the transversal and longitudinal cross sections, by composing them, we can obtain the stress distribution on the semicircular slide. Based on the results, special solutions for establishing the stress distribution between two surfaces without interact in the contact zone have been developed.

Manifold Learning with Self-Organizing Mapping for Feature Extraction of Nonlinear Faults in Rotating Machinery

Directory of Open Access Journals (Sweden)

Lin Liang

2015-01-01

Full Text Available A new method for extracting the low-dimensional feature automatically with self-organization mapping manifold is proposed for the detection of rotating mechanical nonlinear faults (such as rubbing, pedestal looseness. Under the phase space reconstructed by single vibration signal, the self-organization mapping (SOM with expectation maximization iteration algorithm is used to divide the local neighborhoods adaptively without manual intervention. After that, the local tangent space alignment algorithm is adopted to compress the high-dimensional phase space into low-dimensional feature space. The proposed method takes advantages of the manifold learning in low-dimensional feature extraction and adaptive neighborhood construction of SOM and can extract intrinsic fault features of interest in two dimensional projection space. To evaluate the performance of the proposed method, the Lorenz system was simulated and rotation machinery with nonlinear faults was obtained for test purposes. Compared with the holospectrum approaches, the results reveal that the proposed method is superior in identifying faults and effective for rotating machinery condition monitoring.

Vibration analysis for trending ageing in rotating machinery

International Nuclear Information System (INIS)

Sinha, S.K.; Rama Rao, A.

2006-01-01

The need for condition monitoring system for important equipment and machinery is a growing requirement in every industry and more so in the nuclear power plants because of stringent safety requirements. This is largely because of the inherent benefit of being able to promote predictive maintenance practice rather than uneconomical preventive maintenance practice in the plant. Forerunner among the condition monitoring parameter is vibration signatures measured on a rotating machine. It is known that every moving element in a rotating machine generates vibration signal that is uniquely its own. Detection of such signals and monitoring the changing conditions in a machine through vibration analysis is a technique involving the knowledge of engineering art and the mathematical theory. This blend of sound engineering judgement and vibration data interpretation skill is in fact the basis of vibration diagnostic techniques. (author)

ENVIRONMENTAL TECHNOLOGY VERIFICATION REPORT, SWINE WASTE ELECTRIC POWER AND HEAT PRODUCTION--MARTIN MACHINERY INTERNAL COMBUSTION ENGINE

Science.gov (United States)

Under EPA’s Environmental Technology Verification program, which provides objective and scientific third party analysis of new technology that can benefit the environment, a combined heat and power system designed by Martin Machinery was evaluated. This paper provides test result...

Research Using Virtual Reality: Mobile Machinery Safety in the 21st Century

OpenAIRE

Dickey, James; Eger, Tammy; Frayne, Ryan; Delgado, Giselle; Ji, Xiaoxu

2013-01-01

Whole-body vibration is a significant health risk for between 4% and 7% of the work force in North America. In addition, many factors compound the health risks of heavy machinery operators. For example, twisted trunk and neck postures stiffen the spine and increase the transmission of vibration to the head. Similarly, workers adopt awkward postures in order to gain appropriate lines of sight for machine operations. Although the relative contribution of these various issues can be evaluated in...

To Problem of Heat Load Distribution among Boilers with Various Systems for Regulation of Draught Machinery Productivity

Directory of Open Access Journals (Sweden)

V. I. Nazarov

2010-01-01

Full Text Available The paper shows that while distributing loads among boilers an account of the power consumed by draught machinery with various regulation systems shifts an optimum point of load distribution by 2–5%.

Fuel use and emissions from non-road machinery in Denmark from 1985-2004 - and projections from 2005-2030

International Nuclear Information System (INIS)

Winther, M.; Nielsen, Ole-Kenneth

2006-01-01

This report documents the updated 1985-2004 fuel use and emission inventory for non road machinery and recreational craft in Denmark. The inventory comprises the emission components of SO 2 , NO x , NMVOC, CH 4 , CO, CO 2 , N 2 O, NH 3 and TSP, and in addition a fuel use and emission forecast is presented from 2005-2030. The calculated results are grouped into the sub-sectors agriculture, forestry, industry, household/gardening and inland waterways, according to the structure of the CollectER database used for all Danish sources. The report explains the existing EU emission directives for non road machinery, the actual fuel use and emission factors used, sources of background and operational data, calculation methods and the calculated fuel use and emission results. (au)

Effect of machinery wheel load on grass yield

DEFF Research Database (Denmark)

Green, Ole; Jørgensen, Rasmus Nyholm; Kristensen, Kristian

2010-01-01

Effect of machinery wheel load on grass   Ole Green1, Rasmus N. Jørgensen2, Kristian Kristensen3, René Gislum3, Dionysis Bochtis1, & Claus G. Sørensen1   1University of Aarhus, Dept. of Agricultural Engineering 2University of Southern Denmark, Inst. of Chemical Eng., Biotechnology and Environmental...... 3University of Aarhus, Dept. of Genetics and Biotechnology   Corresponding author: Ole Green Address & e-mail: Research Centre Foulum, Blichers Allé 20, 8830 Tjele. Ole.Green@agrsci.dk     Abstract   Different traffic intensities have been shown to have a negative influence on the yield of grass...... and clover. A full scale grass-clover field trial was established to estimate the effect on clover-grass yields as a function of different wheel loads and tire pressures. The trial comprised 16 different traffic intensities with 35 replicates and 1 traffic free treatment with 245 replicates, totalling 17...

Computer System For Diagnostics of Mobile Machinery Transmission

Directory of Open Access Journals (Sweden)

G. L. Antipenko

2004-01-01

Full Text Available A new method for diagnostics of mechanical transmissions of mobile machinery is proposed in the paper. The method presupposes an application of computing equipment and its purpose is to decrease labor-consumption of diagnostics procedure and increase diagnostics efficiency.The method is based on comparison of duration of impulse periods picked up at primary transducers which are installed at transmission input and output. A signal picked up at a flywheel ring gear is taken as a reference signal.While selecting clearances of one and then the direction in speed-up - braking transmission regime changes in number of reference impulses at output provide data on angular clearance value in every gearing. As data are supplied registration and processing of results and forecasting of residual resource are to be done with the help of a program on the basis of realized algorithms for every gearing.

Mechanical design handbook for elastomers. [the design of elastomer dampers for application in rotating machinery

Science.gov (United States)

Darlow, M.; Zorzi, E.

1981-01-01

A comprehensive guide for the design of elastomer dampers for application in rotating machinery is presented. Theoretical discussions, a step by step procedure for the design of elastomer dampers, and detailed examples of actual elastomer damper applications are included. Dynamic and general physical properties of elastomers are discussed along with measurement techniques.

Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery*

Science.gov (United States)

Gakh, Oleksandr; Ranatunga, Wasantha; Smith, Douglas Y.; Ahlgren, Eva-Christina; Al-Karadaghi, Salam; Thompson, James R.; Isaya, Grazia

2016-01-01

Fe-S clusters, essential cofactors needed for the activity of many different enzymes, are assembled by conserved protein machineries inside bacteria and mitochondria. As the architecture of the human machinery remains undefined, we co-expressed in Escherichia coli the following four proteins involved in the initial step of Fe-S cluster synthesis: FXN42–210 (iron donor); [NFS1]·[ISD11] (sulfur donor); and ISCU (scaffold upon which new clusters are assembled). We purified a stable, active complex consisting of all four proteins with 1:1:1:1 stoichiometry. Using negative staining transmission EM and single particle analysis, we obtained a three-dimensional model of the complex with ∼14 Å resolution. Molecular dynamics flexible fitting of protein structures docked into the EM map of the model revealed a [FXN42–210]24·[NFS1]24·[ISD11]24·[ISCU]24 complex, consistent with the measured 1:1:1:1 stoichiometry of its four components. The complex structure fulfills distance constraints obtained from chemical cross-linking of the complex at multiple recurring interfaces, involving hydrogen bonds, salt bridges, or hydrophobic interactions between conserved residues. The complex consists of a central roughly cubic [FXN42–210]24·[ISCU]24 sub-complex with one symmetric ISCU trimer bound on top of one symmetric FXN42–210 trimer at each of its eight vertices. Binding of 12 [NFS1]2·[ISD11]2 sub-complexes to the surface results in a globular macromolecule with a diameter of ∼15 nm and creates 24 Fe-S cluster assembly centers. The organization of each center recapitulates a previously proposed conserved mechanism for sulfur donation from NFS1 to ISCU and reveals, for the first time, a path for iron donation from FXN42–210 to ISCU. PMID:27519411

Biosynthetic machinery of ionophore polyether lasalocid: enzymatic construction of polyether skeleton.

Science.gov (United States)

Minami, Atsushi; Oguri, Hiroki; Watanabe, Kenji; Oikawa, Hideaki

2013-08-01

Diversity of natural polycyclic polyethers originated from very simple yet versatile strategy consisting of epoxidation of linear polyene followed by epoxide opening cascade. To understand two-step enzymatic transformations at molecular basis, a flavin containing monooxygenase (EPX) Lsd18 and an epoxide hydrolase (EH) Lsd19 were selected as model enzymes for extensive investigation on substrate specificity, catalytic mechanism, cofactor requirement and crystal structure. This pioneering study on prototypical lasalocid EPX and EH provides insight into detailed mechanism of ionophore polyether assembly machinery and clarified remaining issues for polyether biosynthesis. Copyright © 2013 Elsevier Ltd. All rights reserved.

Machinery running state identification based on discriminant semi-supervised local tangent space alignment for feature fusion and extraction

International Nuclear Information System (INIS)

Su, Zuqiang; Xiao, Hong; Zhang, Yi; Tang, Baoping; Jiang, Yonghua

2017-01-01

Extraction of sensitive features is a challenging but key task in data-driven machinery running state identification. Aimed at solving this problem, a method for machinery running state identification that applies discriminant semi-supervised local tangent space alignment (DSS-LTSA) for feature fusion and extraction is proposed. Firstly, in order to extract more distinct features, the vibration signals are decomposed by wavelet packet decomposition WPD, and a mixed-domain feature set consisted of statistical features, autoregressive (AR) model coefficients, instantaneous amplitude Shannon entropy and WPD energy spectrum is extracted to comprehensively characterize the properties of machinery running state(s). Then, the mixed-dimension feature set is inputted into DSS-LTSA for feature fusion and extraction to eliminate redundant information and interference noise. The proposed DSS-LTSA can extract intrinsic structure information of both labeled and unlabeled state samples, and as a result the over-fitting problem of supervised manifold learning and blindness problem of unsupervised manifold learning are overcome. Simultaneously, class discrimination information is integrated within the dimension reduction process in a semi-supervised manner to improve sensitivity of the extracted fusion features. Lastly, the extracted fusion features are inputted into a pattern recognition algorithm to achieve the running state identification. The effectiveness of the proposed method is verified by a running state identification case in a gearbox, and the results confirm the improved accuracy of the running state identification. (paper)

Prediction model for sound transmission from machinery in buildings: feasible approaches and problems to be solved

NARCIS (Netherlands)

Gerretsen, E.

2000-01-01

Prediction models for the airborne and impact sound transmission in buildings have recently been established (EN 12354- 1&2:1999). However, these models do not cover technical installations and machinery as a source of sound in buildings. Yet these can cause unacceptable sound levels and it is

Optimization of advenced liquid natural gas-fuelled combined cycle machinery systems for a high-speed ferry

DEFF Research Database (Denmark)

Tveitaskog, Kari Anne; Haglind, Fredrik

2012-01-01

. Furthermore, practical and operational aspects of using these three machinery systems for a high-speed ferry are discussed. Two scenarios are evaluated. The first scenario evaluates the combined cycles with a given power requirement, optimizing the combined cycle while operating the gas turbine at part load...

C4 photosynthetic machinery: insights from maize chloroplast proteomics

Directory of Open Access Journals (Sweden)

Qi eZhao

2013-04-01

Full Text Available C4 plants exhibit much higher CO2 assimilation rates than C3 plants. The specialized differentiation of mesophyll cell (M and bundle sheath cell (BS type chloroplasts is unique to C4 plants and improves photosynthesis efficiency. Maize (Zea mays is an important crop and model with C4 photosynthetic machinery. Current high-throughput quantitative proteomics approaches (e.g., 2DE, iTRAQ, and shotgun proteomics have been employed to investigate maize chloroplast structure and function. These proteomic studies have provided valuable information on C4 chloroplast protein components, photosynthesis, and other metabolic mechanisms underlying chloroplast biogenesis, stromal and membrane differentiation, as well as response to salinity, high/low temperature, and light stress. This review presents an overview of proteomics advances in maize chloroplast biology.

Mutations in SNRPB, encoding components of the core splicing machinery, cause cerebro-costo-mandibular syndrome.

Science.gov (United States)

Bacrot, Séverine; Doyard, Mathilde; Huber, Céline; Alibeu, Olivier; Feldhahn, Niklas; Lehalle, Daphné; Lacombe, Didier; Marlin, Sandrine; Nitschke, Patrick; Petit, Florence; Vazquez, Marie-Paule; Munnich, Arnold; Cormier-Daire, Valérie

2015-02-01

Cerebro-costo-mandibular syndrome (CCMS) is a developmental disorder characterized by the association of Pierre Robin sequence and posterior rib defects. Exome sequencing and Sanger sequencing in five unrelated CCMS patients revealed five heterozygous variants in the small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB) gene. This gene includes three transcripts, namely transcripts 1 and 2, encoding components of the core spliceosomal machinery (SmB' and SmB) and transcript 3 undergoing nonsense-mediated mRNA decay. All variants were located in the premature termination codon (PTC)-introducing alternative exon of transcript 3. Quantitative RT-PCR analysis revealed a significant increase in transcript 3 levels in leukocytes of CCMS individuals compared to controls. We conclude that CCMS is due to heterozygous mutations in SNRPB, enhancing inclusion of a SNRPB PTC-introducing alternative exon, and show that this developmental disease is caused by defects in the splicing machinery. Our finding confirms the report of SNRPB mutations in CCMS patients by Lynch et al. (2014) and further extends the clinical and molecular observations. © 2014 WILEY PERIODICALS, INC.

The pupylation machinery is involved in iron homeostasis by targeting the iron storage protein ferritin.

Science.gov (United States)

Küberl, Andreas; Polen, Tino; Bott, Michael

2016-04-26

The balance of sufficient iron supply and avoidance of iron toxicity by iron homeostasis is a prerequisite for cellular metabolism and growth. Here we provide evidence that, in Actinobacteria, pupylation plays a crucial role in this process. Pupylation is a posttranslational modification in which the prokaryotic ubiquitin-like protein Pup is covalently attached to a lysine residue in target proteins, thus resembling ubiquitination in eukaryotes. Pupylated proteins are recognized and unfolded by a dedicated AAA+ ATPase (Mycobacterium proteasomal AAA+ ATPase; ATPase forming ring-shaped complexes). In Mycobacteria, degradation of pupylated proteins by the proteasome serves as a protection mechanism against several stress conditions. Other bacterial genera capable of pupylation such as Corynebacterium lack a proteasome, and the fate of pupylated proteins is unknown. We discovered that Corynebacterium glutamicum mutants lacking components of the pupylation machinery show a strong growth defect under iron limitation, which was caused by the absence of pupylation and unfolding of the iron storage protein ferritin. Genetic and biochemical data support a model in which the pupylation machinery is responsible for iron release from ferritin independent of degradation.

HYDRAULIC UNITS FOR DRIVING SYSTEMS OF RUNNING EQUIPMENT IN ROAD CONSTRUCTION MACHINERY

Directory of Open Access Journals (Sweden)

A. Ja. Kotlobai

2016-01-01

Full Text Available Operational efficiency of multi-functional road construction machines depends on number of working bodies which are simultaneously performing technological operations. Systems for propulsion pto to the running equipment drive and active working bodies of road construction machines are developing in the way of using three-axis hydraulic drives. When designing a hydraulic system for road construction machinery dividing of power flow from propulsion to the running equipment drive and active working bodies is considered as rather essential problem. Leading companies do not pay attention to the development of flow divider designs, preferring to produce more expensive multi-flow pumps. One of the ways to increase efficiency of multi-functional road construction machinery is an implementation of running equipment hydraulic driving system based on a mono-aggregate pump unit which consists of a pump and a volumetric divider of power fluid flow. A principle of volumetric division and summing-up of power fluid flows, technical realization and methodology for calculation of key parameters of discrete flow distributors has been developed on the basis of discrete hydraulics regulations. The paper presents results of mathematical modeling of hydraulic systems equipped with the discrete flow distributor. Analysis of a dual-motor hydraulic drive operation has shown the following results: a discrete flow distributor ensures independent load mode of the current consumer circuit operation from the load mode of the second consumer circuit within a wide range of loads; rational value of working fluid flow discretization parameter is the following value interval k = 4–6, maximum value of parameter efficiency is reached when an angular velocity of a distributor rotor coincides with the angular velocity of a pump shaft; discrete flow distributor provides a possibility to change parameters of hydraulic flow feeding in consumers’ pressure lines within a wide range

Fuel use and emissions from non-road machinery in Denmark from 1985-2004 - and projections from 2005-2030

Energy Technology Data Exchange (ETDEWEB)

Winther, M.; Nielsen, Ole-Kenneth [National Environmental Research Inst. (Denmark)

2006-08-31

This report documents the updated 1985-2004 fuel use and emission inventory for non road machinery and recreational craft in Denmark. The inventory comprises the emission components of SO{sub 2}, NO{sub x}, NMVOC, CH{sub 4}, CO, CO{sub 2}, N{sub 2}O, NH{sub 3} and TSP, and in addition a fuel use and emission forecast is presented from 2005-2030. The calculated results are grouped into the sub-sectors agriculture, forestry, industry, household/gardening and inland waterways, according to the structure of the CollectER database used for all Danish sources. The report explains the existing EU emission directives for non road machinery, the actual fuel use and emission factors used, sources of background and operational data, calculation methods and the calculated fuel use and emission results. (au)

MRP transporters as membrane machinery in the bradykinin-inducible export of ATP.

Science.gov (United States)

Zhao, Yumei; Migita, Keisuke; Sun, Jing; Katsuragi, Takeshi

2010-04-01

Adenosine triphosphate (ATP) plays the role of an autocrine/paracrine signal molecule in a variety of cells. So far, however, the membrane machinery in the export of intracellular ATP remains poorly understood. Activation of B2-receptor with bradykinin-induced massive release of ATP from cultured taenia coli smooth muscle cells. The evoked release of ATP was unaffected by gap junction hemichannel blockers, such as 18alpha-glycyrrhetinic acid and Gap 26. Furthermore, the cystic fibrosis transmembrane regulator (CFTR) coupled Cl(-) channel blockers, CFTR(inh)172, 5-nitro-2-(3-phenylpropylamino)-benzoic acid, Gd3(+) and glibenclamide, failed to suppress the export of ATP by bradykinin. On the other, the evoked release of ATP was greatly reduced by multidrug resistance protein (MRP) transporter inhibitors, MK-571, indomethacin, and benzbromarone. From western blotting analysis, blots of MRP 1 protein only, but not MRP 2 and MRP 3 protein, appeared at 190 kD. However, the MRP 1 protein expression was not enhanced after loading with 1 muM bradykinin for 5 min. Likewise, niflumic acid and fulfenamic acid, Ca2(+)-activated Cl(-) channel blockers, largely abated the evoked release of ATP. The possibility that the MRP transporter system couples with Ca2(+)-activated Cl(-) channel activities is discussed here. These findings suggest that MRP transporters, probably MRP 1, unlike CFTR-Cl(-) channels and gap junction hemichannels, may contribute as membrane machinery to the export of ATP induced by G-protein-coupled receptor stimulation.

Construction of a Vibration Monitoring System for HANARO's Rotating Machinery and Analysis of Pump Vibration Signals

International Nuclear Information System (INIS)

Ryu, Jeong Soo; Yoon, Doo Byung

2005-01-01

HANARO is an open-tank-in-pool type research reactor with a thermal power of 30MW. In order to remove the heat generated by the reactor core and the reflector vessel, primary cooling pumps and reflector cooling pumps circulate coolant. These pumps are installed at the RCI(Reactor Concrete Island) which is covered by heavy concrete hatches. For the prevention of an abnormal operation of these pumps in the RCI, it is necessary to construct a vibration monitoring system that provides an alarm signal to the reactor control room when the rotating speed or the vibration level exceeds the allowable limit. The first objective of this work is to construct a vibration monitoring system for HANARO's rotating machinery. The second objective is to verify the possibility of condition monitoring of the rotating machinery. To construct a vibration monitoring system, as a first step, the standards and references related to the vibration monitoring system were investigated. In addition, to determine the number and the location of sensors that can effectively characterize the overall vibration of a pump, the vibration of the primary cooling pumps and the reflector cooling pumps were measured. Based on these results, detailed construction plans for the vibration monitoring system for HANARO were established. Then, in accordance with the construction plans, the vibration monitoring system for HANARO's rotating machinery was manufactured and installed at HANARO. To achieve the second objective, FFT analysis and bearing fault detection of the measured vibration signals were performed. The analysis results demonstrate that the accelerometers mounted at the bearing locations of the pumps can effectively monitor the pump condition

INTEGRATED MANAGEMENT SYSTEM FOR A SOCIETY FROM INDUSTRY OF MACHINERY AND EQUIPMENT

Directory of Open Access Journals (Sweden)

Constanţa RĂDULESCU

2014-05-01

Full Text Available In this paper shows the importance that it has SMI in a society of the industry of machinery and equipment. An integrated management system is the mode to design, implement and certify two or more management systems: Quality - Environment, Quality -Environment - Safety and Occupational Health. By implementing the system, the society themselves hires in the development and continuous improvement of the system in accordance with the ones three standards and with objective of activity of the society. Also, in the paper themselves presents the advantages and disadvantages of implementing this integrated system.

Prognostics and health management design for rotary machinery systems—Reviews, methodology and applications

Science.gov (United States)

Lee, Jay; Wu, Fangji; Zhao, Wenyu; Ghaffari, Masoud; Liao, Linxia; Siegel, David

2014-01-01

Much research has been conducted in prognostics and health management (PHM), an emerging field in mechanical engineering that is gaining interest from both academia and industry. Most of these efforts have been in the area of machinery PHM, resulting in the development of many algorithms for this particular application. The majority of these algorithms concentrate on applications involving common rotary machinery components, such as bearings and gears. Knowledge of this prior work is a necessity for any future research efforts to be conducted; however, there has not been a comprehensive overview that details previous and on-going efforts in PHM. In addition, a systematic method for developing and deploying a PHM system has yet to be established. Such a method would enable rapid customization and integration of PHM systems for diverse applications. To address these gaps, this paper provides a comprehensive review of the PHM field, followed by an introduction of a systematic PHM design methodology, 5S methodology, for converting data to prognostics information. This methodology includes procedures for identifying critical components, as well as tools for selecting the most appropriate algorithms for specific applications. Visualization tools are presented for displaying prognostics information in an appropriate fashion for quick and accurate decision making. Industrial case studies are included in this paper to show how this methodology can help in the design of an effective PHM system.

A new structural framework for integrating replication protein A into DNA processing machinery

Energy Technology Data Exchange (ETDEWEB)

Brosey, Chris; Yan, Chunli; Tsutakawa, Susan; Heller, William; Rambo, Robert; Tainer, John; Ivanov, Ivaylo; Chazin, Walter

2013-01-17

By coupling the protection and organization of single-stranded DNA (ssDNA) with recruitment and alignment of DNA processing factors, replication protein A (RPA) lies at the heart of dynamic multi-protein DNA processing machinery. Nevertheless, how RPA coordinates biochemical functions of its eight domains remains unknown. We examined the structural biochemistry of RPA's DNA-binding activity, combining small-angle X-ray and neutron scattering with all-atom molecular dynamics simulations to investigate the architecture of RPA's DNA-binding core. The scattering data reveal compaction promoted by DNA binding; DNA-free RPA exists in an ensemble of states with inter-domain mobility and becomes progressively more condensed and less dynamic on binding ssDNA. Our results contrast with previous models proposing RPA initially binds ssDNA in a condensed state and becomes more extended as it fully engages the substrate. Moreover, the consensus view that RPA engages ssDNA in initial, intermediate and final stages conflicts with our data revealing that RPA undergoes two (not three) transitions as it binds ssDNA with no evidence for a discrete intermediate state. These results form a framework for understanding how RPA integrates the ssDNA substrate into DNA processing machinery, provides substrate access to its binding partners and promotes the progression and selection of DNA processing pathways.

Emerging roles of the spliceosomal machinery in myelodysplastic syndromes and other hematological disorders.

Science.gov (United States)

Visconte, V; Makishima, H; Maciejewski, J P; Tiu, R V

2012-12-01

In humans, the majority of all protein-coding transcripts contain introns that are removed by mRNA splicing carried out by spliceosomes. Mutations in the spliceosome machinery have recently been identified using whole-exome/genome technologies in myelodysplastic syndromes (MDS) and in other hematological disorders. Alterations in splicing factor 3 subunit b1 (SF3b1) were the first spliceosomal mutations described, immediately followed by identification of other splicing factor mutations, including U2 small nuclear RNA auxillary factor 1 (U2AF1) and serine arginine-rich splicing factor 2 (SRSF2). SF3b1/U2AF1/SRSF2 mutations occur at varying frequencies in different disease subtypes, each contributing to differences in survival outcomes. However, the exact functional consequences of these spliceosomal mutations in the pathogenesis of MDS and other hematological malignancies remain largely unknown and subject to intense investigation. For SF3b1, a gain of function mutation may offer the promise of new targeted therapies for diseases that carry this molecular abnormality that can potentially lead to cure. This review aims to provide a comprehensive overview of the emerging role of the spliceosome machinery in the biology of MDS/hematological disorders with an emphasis on the functional consequences of mutations, their clinical significance, and perspectives on how they may influence our understanding and management of diseases affected by these mutations.

Emerging roles of the spliceosomal machinery in myelodysplastic syndromes and other hematologic disorders

Science.gov (United States)

Visconte, V; Makishima, H; Maciejewski, JP; Tiu, RV

2013-01-01

In humans, the majority of all protein-coding transcripts contain introns that are removed by mRNA splicing carried out by spliceosomes. Mutations in the spliceosome machinery have recently been identified using whole exome/genome technologies in myelodysplastic syndromes (MDS) and in other hematologic disorders. Alterations in Splicing Factor 3 Subunit b1 (SF3b1) were the first spliceosomal mutations described, immediately followed by identification of other splicing factor mutations, including U2 Small Nuclear RNA Auxillary Factor 1 (U2AF1) and Serine Arginine Rich Splicing Factor 2 (SRSF2). SF3b1/U2AF1/SRSF2 mutations occur at varying frequencies in different disease subtypes, each contributing to differences in survival outcomes. However, the exact functional consequences of these spliceosomal mutations in the pathogenesis of MDS and other hematologic malignancies remain largely unknown and subject to intense investigation. For SF3b1, a gain of function mutation may offer the promise of new targeted therapies for diseases that carry this molecular abnormality that can potentially lead to cure. This review aims to provide a comprehensive overview of the emerging role of the spliceosome machinery in the biology of MDS/hematologic disorders with an emphasis on the functional consequences of mutations, their clinical significance, and perspectives on how they may influence our understanding and management of diseases affected by these mutations. PMID:22678168

Structural Insights Into The Bacterial Carbon-Phosphorus Lyase Machinery

DEFF Research Database (Denmark)

Brodersen, Ditlev Egeskov

the proteins encoded in the phn operon act in concert to catabolise phosphonate remain unknown. We have determined the crystal structure of a 240 kDa Escherichia coli carbon-phosphorus lyase core complex at 1.7 Å and show that it comprises a highly intertwined network of subunits with several unexpected......Phosphonate compounds act as a nutrient source for some microorganisms when phosphate is limiting but require a specialised enzymatic machinery due to the presence of the highly stable carbon-phosphorus bond. Despite the fundamental importance to microbial metabolism, the details of how...... structural features. The complex contains at least two different active sites and suggest a revision of current models of carbon-phosphorus bond cleavage. Using electron microscopy, we map the binding site of an additional protein subunit, which may use ATP for driving conformational changes during...

Skoda Concern's cooperation with State Machinery Design Research Institute

International Nuclear Information System (INIS)

Valchar, J.; Kuhn, L.

1988-01-01

The main areas are presented of cooperation between the Skoda Plzen Concern and the State Machinery Design Research Institute in Prague-Bechovice. This is mainly the development of steam turbines, from 50 MW turbines to the present 1000 MW saturated steam turbines designed for nuclear power plants. Main attention is centred on conditions of the boiling crisis in the steam turbine circuit, and its consequences. This study is served by the experimental equipment of the institute and its computer. The cooperation of the two institutions in the field of testing and diagnostic equipment is centred on measuring natural oscillations of turbine blades, the diagnostics of vibrations of steam turbines, the measurement of the humidity of saturated steam, optical measurements of the parameters of saturated steam, ultrasound diagnostics and the measurement of turbine blade deformation caused by hydraulic effects. (Z.M.). 8 figs

Stepwise Diagnosis for Rotating Machinery Using Force Identification Approach

Directory of Open Access Journals (Sweden)

Shozo Kawamura

2012-01-01

Full Text Available Machine condition monitoring and diagnosis have become increasingly important, and the application of these processes has been widely investigated. The authors previously proposed a stepwise diagnosis method for a beam structure. In that method, the location of the abnormality is first estimated using the force identification approach, and then the cause of the abnormality is identified. In this study, the stepwise diagnosis method was improved specifically for rotating machinery. The applicability of the proposed method was checked by using the experimental data. In the case of a rotor system with unbalance, it was shown that the location of the abnormality and its severity could be identified, and, in the case of a rotor system with stationary rubbing, the location of the abnormality could be accurately identified. Therefore, it was confirmed that the proposed diagnostic method is feasible for actual application.

Recent evolutions of refrigerating machineries and heat pumps; Evolutions recentes des machines a froid et thermopompes

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-12-31

This book of proceedings reports on 10 papers (or series of transparencies) concerning some recent developments about refrigerating machineries and heat pumps as used in space heating, air-conditioning and industrial refrigeration. Various aspects are developed: thermodynamic cycles, thermal performances, dimensioning, modeling, refrigerants substitution, design of flanged exchangers, compressors etc.. (J.S.)

46 CFR 167.65-70 - Reports of accidents, repairs, and unsafe boilers and machinery by engineers.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Reports of accidents, repairs, and unsafe boilers and... of accidents, repairs, and unsafe boilers and machinery by engineers. (a) Before making repairs to a boiler of a nautical school ship the engineer in charge shall report, in writing, the nature of such...

Recent evolutions of refrigerating machineries and heat pumps; Evolutions recentes des machines a froid et thermopompes

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-12-31

This book of proceedings reports on 10 papers (or series of transparencies) concerning some recent developments about refrigerating machineries and heat pumps as used in space heating, air-conditioning and industrial refrigeration. Various aspects are developed: thermodynamic cycles, thermal performances, dimensioning, modeling, refrigerants substitution, design of flanged exchangers, compressors etc.. (J.S.)

Engineering yeast transcription machinery for improved ethanol tolerance and production.

Science.gov (United States)

Alper, Hal; Moxley, Joel; Nevoigt, Elke; Fink, Gerald R; Stephanopoulos, Gregory

2006-12-08

Global transcription machinery engineering (gTME) is an approach for reprogramming gene transcription to elicit cellular phenotypes important for technological applications. Here we show the application of gTME to Saccharomyces cerevisiae for improved glucose/ethanol tolerance, a key trait for many biofuels programs. Mutagenesis of the transcription factor Spt15p and selection led to dominant mutations that conferred increased tolerance and more efficient glucose conversion to ethanol. The desired phenotype results from the combined effect of three separate mutations in the SPT15 gene [serine substituted for phenylalanine (Phe(177)Ser) and, similarly, Tyr(195)His, and Lys(218)Arg]. Thus, gTME can provide a route to complex phenotypes that are not readily accessible by traditional methods.

Modulation of DNA methylation machineries in japanese rice fish (Oryzias latipes) embryogenesis by ethanol and 5-azacytidine

Science.gov (United States)

As a sequel of our investigations on the impact of epigenome in inducing fetal alcohol spectrum disorder (FASD) phenotypes in Japanese rice fish, we investigated on several DNA methylation machinery genes including DNA methyl transferase 3ba (dnmt3ba) and methyl binding proteins (MBPs), namely, mbdl...

Restoration of GABA production machinery in Lactobacillus brevis by accessible carbohydrates, anaerobiosis and early acidification.

Science.gov (United States)

Wu, Qinglong; Shah, Nagendra P

2018-02-01

Lactobacillus brevis is an efficient cell factory for producing bioactive γ-aminobutyric acid (GABA) by its gad operon-encoded glutamic acid decarboxylase (GAD) system. However, little mechanistic insights have been reported on the effects of carbohydrate, oxygen and early acidification on GABA production machinery in Lb. brevis. In the present study, GABA production from Lb. brevis was enhanced by accessible carbohydrates. Fast growth of this organism was stimulated by maltose and xylose. However, its GABA production was highly suppressed by oxygen exposure, but was fully restored by anaerobiosis that up-regulated the expression of gad operon in Lb. brevis cells. Although the level of cytosolic acidity was suitable for the functioning of GadA and GadB, early acidification of the medium (ipH 5 and ipH 4) restored GABA synthesis strictly in aerated cells of Lb. brevis because the expression of gad operon was not up-regulated in them. We conclude that GABA production machinery in Lb. brevis could be restored by accessible carbohydrates, anaerobiosis and early acidification. This will be of interest for controlling fermentation for synthesis of GABA and manufacturing GABA-rich fermented vegetables. Copyright © 2017. Published by Elsevier Ltd.

Protein-protein interactions within the ensemble, eukaryotic V-ATPase, and its concerted interactions with cellular machineries.

Science.gov (United States)

Balakrishna, Asha Manikkoth; Manimekalai, Malathy Sony Subramanian; Grüber, Gerhard

2015-10-01

The V1VO-ATPase (V-ATPase) is the important proton-pump in eukaryotic cells, responsible for pH-homeostasis, pH-sensing and amino acid sensing, and therefore essential for cell growths and metabolism. ATP-cleavage in the catalytic A3B3-hexamer of V1 has to be communicated via several so-called central and peripheral stalk units to the proton-pumping VO-part, which is membrane-embedded. A unique feature of V1VO-ATPase regulation is its reversible disassembly of the V1 and VO domain. Actin provides a network to hold the V1 in proximity to the VO, enabling effective V1VO-assembly to occur. Besides binding to actin, the 14-subunit V-ATPase interacts with multi-subunit machineries to form cellular sensors, which regulate the pH in cellular compartments or amino acid signaling in lysosomes. Here we describe a variety of subunit-subunit interactions within the V-ATPase enzyme during catalysis and its protein-protein assembling with key cellular machineries, essential for cellular function. Copyright © 2015 Elsevier Ltd. All rights reserved.

Automation of mining machinery at RAG; Automation von Bergbaumaschinen bei der RAG Deutsche Steinkohle

Energy Technology Data Exchange (ETDEWEB)

Barabasch, Uwe [Zentralstab Kernbereich, RAG Deutsche Steinkohle AG, Herne (Germany); Weiss, Hans-Juergen [Bergwerk Prosper-Haniel, RAG Deutsche Steinkohle AG, Bottrop (Germany); Kotke, Frank [Elektrotechnik unter Tage, Zentralstab Kernbereich der RAG Deutsche Steinkohle AG, Herne (Germany)

2009-11-05

The improvement of processes specific to mining in the collieries of RAG and the improvement of the ergonomic conditions in the deep coal mining deposits of Germany require a higher degree of automation and control of the processes in progress. A higher degree of automation is also re-quired here for the machinery and systems used. RAG will be consolidating its engineering and research activities in these areas over the coming years. (orig.)

The National Shipbuilding Research Program, 1997 Ship Production Symposium, Paper No. 21: A Parametric Approach to Machinery Unitization in Shipbuilding

National Research Council Canada - National Science Library

Jaquith, Peter E; Burns, Richard M; Duneclift, Lee A; Gaskari, Massood; Green, Thomas; Silveira, John L; Walsh, Anthony

1997-01-01

During the past ten years, both U.S. and foreign shipyards have developed advanced unitization concepts that include multi-level assemblies representing large vertical segments of ship machinery spaces...

Electric machinery and drives in thermal power stations. Elektrische Maschinen und Antriebe in thermischen Kraftwerken

Energy Technology Data Exchange (ETDEWEB)

1974-01-01

The following subjects were dealt with during the VDE meeting: 1) Requirements made by the electric network on the generators and their excitation equipment, and the influence thereof on their design; 2) requirements made by the power station process on the electric drives and the influence thereof on type and design; 3) requirements made on protective measures from the point of the electric power station machinery.

Energy machineries on a manifold: Application to the construction of new energy representations of gauge groups

International Nuclear Information System (INIS)

Marion, J.

1984-01-01

The introduction of the concepts of energy machinery and energy structure of a manifold allows to construct a large class of energy representations of gauge groups including, as a very particular case, the ones known up to now. A synthesis of earlier works allows to give a sufficient condition for the irreducibility of these representations. (orig./HSI)

Dengue virus-induced regulation of the host cell translational machinery

Directory of Open Access Journals (Sweden)

C.S.A. Villas-Bôas

2009-11-01

Full Text Available Dengue virus (DV-induced changes in the host cell protein synthesis machinery are not well understood. We investigated the transcriptional changes related to initiation of protein synthesis. The human hepatoma cell line, HepG2, was infected with DV serotype 2 for 1 h at a multiplicity of infection of one. RNA was extracted after 6, 24 and 48 h. Microarray results showed that 36.5% of the translation factors related to initiation of protein synthesis had significant differential expression (Z-score ≥ ±2.0. Confirmation was obtained by quantitative real-time reverse transcription-PCR. Of the genes involved in the activation of mRNA for cap-dependent translation (eIF4 factors, eIF4A, eIF4G1 and eIF4B were up-regulated while the negative regulator of translation eIF4E-BP3 was down-regulated. This activation was transient since at 24 h post-infection levels were not significantly different from control cells. However, at 48 h post-infection, eIF4A, eIF4E, eIF4G1, eIF4G3, eIF4B, and eIF4E-BP3 were down-regulated, suggesting that cap-dependent translation could be inhibited during the progression of infection. To test this hypothesis, phosphorylation of p70S6K and 4E-BP1, which induce cap-dependent protein synthesis, was assayed. Both proteins remained phosphorylated when assayed at 6 h after infection, while infection induced dephosphorylation of p70S6K and 4E-BP1 at 24 and 48 h of infection, respectively. Taken together, these results provide biological evidence suggesting that in HepG2 cells DV sustains activation of the cap-dependent machinery at early stages of infection, but progression of infection switches protein synthesis to a cap-independent process.

Design and modeling of an advanced marine machinery system including waste heat recovery and removal of sulphur oxides

DEFF Research Database (Denmark)

Frimann Nielsen, Rasmus; Haglind, Fredrik; Larsen, Ulrik

2014-01-01

the efficiency of machinery systems. The wet sulphuric acid process is an effective way of removing flue gas sulphur oxides from land-based coal-fired power plants. Moreover, organic Rankine cycles (ORC) are suitable for heat to power conversion for low temperature heat sources. This paper describes the design...... that an ORC placed after the conventional waste heat recovery system is able to extract the sulphuric acid from the exhaust gas, while at the same time increase the combined cycle thermal efficiency by 2.6%. The findings indicate that the technology has potential in marine applications regarding both energy...... and modeling of a highly efficient machinery system which includes the removal of exhaust gas sulphur oxides. The system consists of a two-stroke diesel engine, the wet sulphuric process for sulphur removal, a conventional steam Rankine cycle and an ORC. Results of numerical modeling efforts suggest...

Customer relationship management in the agricultural machinery market

Directory of Open Access Journals (Sweden)

Amanda Letícia Pit Nunes

Full Text Available ABSTRACT: Customer Relationship Management can be regarded as a business approach. The objective was to know the customers, meet their expectations, and thus build customer loyalty. Although, the agricultural sector makes significant economic contributions to the Brazilian market and induces sharp competition among its companies, a huge opportunity still presents itself for the diffusion and implementation of CRM in the agricultural machinery sector. This study aimed to highlight the importance of customer management, by introducing the customer relationship management (CRM concept. This is possible in the event of reselling agricultural machines, with the intention of retaining the customers and raising the profitability of these companies. It is necessary to understand CRM as more than a mere a concept or a tool. It is a business strategy, an endeavor that must be endorsed by the entire company. The concessionaire must be perceived as greater than a mere reseller. It is to be viewed rather as a problem solver, as one who offers services that are high in quality and meet client specifics.

Neural networks for the monitoring of rotating machinery

International Nuclear Information System (INIS)

Alguindigue, I.E.; Loskiewicz-Buczak

1991-01-01

Vibration monitoring of components in engineering systems and plants involves the collection of vibration data and detailed analysis to detect features which reflect the operational state of the machinery. The analysis leads to the identification of potential failures and their causes, and makes it possible to perform efficient preventive maintenance. This paper describes a methodology for the automation of some of the activities related to motion and vibration monitoring in these systems. The technique involves training a neural network to model the inter- relationship between signals from two related sensors mounted on an engineering system or component at a time when it is known to be operating properly. Then one signal (or its characteristics) is put into the neural network model to predict the second signal (or its characteristics). This predicted signal is continuously compared with the actual signal A deviation between the predicted and actual signal indicates a changing relationship, usually failure of the component or system. This deviation may be quantified and provides meaningful information about the degree of degradation and deterioration of the component

Verification of the machinery condition monitoring technology by fault simulation tests

International Nuclear Information System (INIS)

Maehara, Takafumi; Watanabe, Yukio; Osaki, Kenji; Higuma, Koji; Nakano, Tomohito

2009-01-01

This paper shows the test items and equipments introduced by Japan Nuclear Energy Safety Organization to establish the monitoring technique for machinery conditions. From the result of vertical pump simulation tests, it was confirmed that fault analysis was impossible by measuring the accelerations on both motor and pump column pipes, however, was possible by measuring of pump shaft vibrations. Because hydraulic whirls by bearing wear had significant influences over bearing misalignments and flow rates, the monitoring trends must be done under the same condition (on bearing alignments and flow rates). We have confirmed that malfunctions of vertical pumps can be diagnosed using measured shaft vibration by ultrasonic sensors from outer surface of pump casing on the floor. (author)

10th International Conference on Vibration Engineering and Technology of Machinery

CERN Document Server

2015-01-01

The VETOMAC-X Conference covered a holistic plethora of relevant topics in vibration and engineering technology including condition monitoring, machinery and structural dynamics, rotor dynamics,  experimental techniques, finite element model updating, industrial case studies, vibration control and energy harvesting, and signal processing. These proceedings contain not only all of the nearly one-hundred peer-reviewed presentations from authors representing more than twenty countries, but also include six invited lectures from renowned experts: Professor K. Gupta, Mr W. Hahn, Professor A.W. Lees, Professor John Mottershead, Professor J.S. Rao, and Dr P. Russhard. This work is of interest to researchers and practitioners alike, and is an essential book for most of libraries of higher academic institutes.

Conservation of proteo-lipid nuclear membrane fusion machinery during early embryogenesis.

Science.gov (United States)

Byrne, Richard D; Veeriah, Selvaraju; Applebee, Christopher J; Larijani, Banafshé

2014-01-01

The fusogenic lipid diacylglycerol is essential for remodeling gamete and zygote nuclear envelopes (NE) during early embryogenesis. It is unclear whether upstream signaling molecules are likewise conserved. Here we demonstrate PLCγ and its activator SFK1, which co-operate during male pronuclear envelope formation, also promote the subsequent male and female pronuclear fusion. PLCγ and SFK1 interact directly at the fusion site leading to PLCγ activation. This is accompanied by a spatially restricted reduction of PtdIns(4,5)P2. Consequently, pronuclear fusion is blocked by PLCγ or SFK1 inhibition. These findings identify new regulators of events in the early embryo and suggest a conserved "toolkit" of fusion machinery drives successive NE fusion events during embryogenesis.

Mechatronics Applied to Fluid Film Bearings: Towards More Efficient Machinery

DEFF Research Database (Denmark)

Cerda Varela, Alejandro Javier

the machine is defined as a mechatronic system. This integrated approach comprises the usage of machine elements capable of modifying their characteristics, by using in a combined way mechanical elements, sensors, processing units and actuators. The research project entitled "Mechatronics Applied to Fluid......The current trends regarding turbomachinery design and operation demand for an expansion of the operational boundaries of these mechanical systems, regarding production rate, reliability and adaptability. In order to face the new requirements, it is necessary to migrate towards a new concept, where...... Film Bearings: Towards more Efficient Machinery" was aimed at improving the state of the art regarding the usage of fluid film bearings as "smart" machine elements. Specifically, this project dealt with a tilting pad journal bearing design that features a controllable lubrication system, capable...

Assessment of the Vibrations Effects Caused by Technical Seismicity Due to the Railway traffic on High-sensitivity Machinery

Science.gov (United States)

Papán, Daniel; Valašková, Veronika; Demeterová, Katarína

2016-10-01

The numerical and experimental approach in structural dynamics problems is more and more current nowadays. This approach is applied and solved in many research and developing institutions of the all the world. Vibrations effect caused by passing trains used in manufacturing facilities can affect the quality of the production activity. This effect is possible to be solved by a numerical or an experimental way. Numerical solution is not so financially and time demanding. The main aim of this article is to focus on just experimental measurement of this problem. In this paper, the case study with measurement due to cramped conditions realized in situ is presented. The case study is located close to railway. The vibration effect caused by passing trains on the high-sensitivity machinery contained in this object were observed. The structure was a high-sensitivity machine that was placed in a construction process. For the measurements, the high-sensitivity standard vibrations equipment was used. The assessments of measurements’ results were performed for the technological conditions and Slovak Standard Criteria. Both of these assessments were divided to amplitude and frequency domain. The amplitude criterion is also divided to peak particle velocity and RMS (Root Mean Square). Frequency domain assessment were realised using the frequency response curves obtained from high-sensitivity machinery manufacturer. The frequency limits are established for each axis of triaxle system. The measurement results can be predicted if the vibration have to be reduced. Measurement implemented in the production hall should obtain materials to determine the seismic loading and response of production machinery caused by technical seismicity.

The development of natural circulation operation support program for ship nuclear power machinery

International Nuclear Information System (INIS)

Hao, Jianli; Chen, Wenzhen; Chen, Zhiyun

2012-01-01

Highlights: ► The natural circulation under various ocean and ship motion conditions is studied. ► A natural circulation operation support computer program (NCOSP) is developed with Simulink. ► The NCOSP program has the merit of easy input preparation, fast and accurate simulation. ► The NCOSP is suitable for the fast parameter simulation of ship nuclear power machinery. -- Abstract: The existing simulation program of ship nuclear power machinery (SNPM) cannot adequately deal with the natural circulation (NC) operation and the effects of various ocean conditions and ship motion. Aiming at the problem, the natural circulation operation support computer program for SNPM is developed, in which the momentum conservation equation of the primary loop, some heat transfer and flow resistance models and equations are modified for the various ocean conditions and ship motion. The additional pressure loss model and effective height model for the control volume in the gyration movement, simple harmonic rolling and pitching movements are also discussed in the paper. Furthermore, the transient response to load change under NC conditions is analyzed by the developed program. The results are compared with those predicted by the modified RELAP5/mod3.2 code. It is shown that the natural circulation operation support program (NCOSP) is simple in the input preparation, runs fast and has a satisfactory precision, and is therefore very suitable for the operating field support of SNPM under the conditions of NC.

Characterization and transcriptional analysis of two gene clusters for type IV secretion machinery in Wolbachia of Armadillidium vulgare

DEFF Research Database (Denmark)

Félix, Christine; Pichon, Samuel; Braquart-Varnier, Christine

2008-01-01

Wolbachia are maternally inherited alpha-proteobacteria that induce feminization of genetic males in most terrestrial crustacean isopods. Two clusters of vir genes for a type IV secretion machinery have been identified at two separate loci and characterized for the first time in a feminizing Wolb...

Immunoglobulin E-mediated sensitization to pine and beech dust in relation to wood dust exposure levels and respiratory symptoms in the furniture industry.

Science.gov (United States)

Schlünssen, Vivi; Kespohl, Sabine; Jacobsen, Gitte; Raulf-Heimsoth, Monika; Schaumburg, Inger; Sigsgaard, Torben

2011-03-01

Wood dust exposure may cause Immunoglobulin E (IgE)-mediated allergic diseases. Our objectives were to estimate pine and beech dust sensitization rates among woodworkers and a reference group, explore the association between exposure and sensitization and between sensitization and respiratory symptoms, and finally investigate the impact of proteinogenic specific IgE (sIgE) epitopes on respiratory symptoms. In a Danish study among 52 furniture factories and 2 reference factories, we evaluated the workers' asthma and rhinitis status using questionnaires and blood samples collected from 1506 woodworkers and 195 references. Workers with asthma symptoms (N=298), a random study sample (N=399) and a random rhinitis sample (N=100) were evaluated for IgE-mediated sensitization to pine and beech dust. The prevalence of pine and beech sensitization among current woodworkers was 1.7 and 3.1%, respectively. No differences in sensitization rates were found between woodworkers and references, but the prevalence of wood dust sensitization was dose-dependently associated with the current level of wood dust exposure. No relation was observed between wood dust sensitization per se and respiratory symptoms. Only symptomatic subjects had proteinogenic IgE epitopes to pine. Increased odds ratios for sIgE based on proteinogenic epitopes to beech and respiratory symptoms were found, although they were not statistically significant. Sensitization rates to pine and beech were the same for woodworkers and references but dependent on the current wood dust exposure level. The importance of beech and pine wood sensitization is limited, but may be of clinical significance for a few workers if the IgE epitopes are proteinogenic.

Occupational Accidents with Agricultural Machinery in Austria.

Science.gov (United States)

Kogler, Robert; Quendler, Elisabeth; Boxberger, Josef

2016-01-01

The number of recognized accidents with fatalities during agricultural and forestry work, despite better technology and coordinated prevention and trainings, is still very high in Austria. The accident scenarios in which people are injured are very different on farms. The common causes of accidents in agriculture and forestry are the loss of control of machine, means of transport or handling equipment, hand-held tool, and object or animal, followed by slipping, stumbling and falling, breakage, bursting, splitting, slipping, fall, and collapse of material agent. In the literature, a number of studies of general (machine- and animal-related accidents) and specific (machine-related accidents) agricultural and forestry accident situations can be found that refer to different databases. From the database Data of the Austrian Workers Compensation Board (AUVA) about occupational accidents with different agricultural machinery over the period 2008-2010 in Austria, main characteristics of the accident, the victim, and the employer as well as variables on causes and circumstances by frequency and contexts of parameters were statistically analyzed by employing the chi-square test and odds ratio. The aim of the study was to determine the information content and quality of the European Statistics on Accidents at Work (ESAW) variables to evaluate safety gaps and risks as well as the accidental man-machine interaction.

Plant Virus Infection and the Ubiquitin Proteasome Machinery: Arms Race along the Endoplasmic Reticulum.

Science.gov (United States)

Verchot, Jeanmarie

2016-11-19

The endoplasmic reticulum (ER) is central to plant virus replication, translation, maturation, and egress. Ubiquitin modification of ER associated cellular and viral proteins, alongside the actions of the 26S proteasome, are vital for the regulation of infection. Viruses can arrogate ER associated ubiquitination as well as cytosolic ubiquitin ligases with the purpose of directing the ubiquitin proteasome system (UPS) to new targets. Such targets include necessary modification of viral proteins which may stabilize certain complexes, or modification of Argonaute to suppress gene silencing. The UPS machinery also contributes to the regulation of effector triggered immunity pattern recognition receptor immunity. Combining the results of unrelated studies, many positive strand RNA plant viruses appear to interact with cytosolic Ub-ligases to provide novel avenues for controlling the deleterious consequences of disease. Viral interactions with the UPS serve to regulate virus infection in a manner that promotes replication and movement, but also modulates the levels of RNA accumulation to ensure successful biotrophic interactions. In other instances, the UPS plays a central role in cellular immunity. These opposing roles are made evident by contrasting studies where knockout mutations in the UPS can either hamper viruses or lead to more aggressive diseases. Understanding how viruses manipulate ER associated post-translational machineries to better manage virus-host interactions will provide new targets for crop improvement.

Plant Virus Infection and the Ubiquitin Proteasome Machinery: Arms Race along the Endoplasmic Reticulum

Directory of Open Access Journals (Sweden)

Jeanmarie Verchot

2016-11-01

Full Text Available The endoplasmic reticulum (ER is central to plant virus replication, translation, maturation, and egress. Ubiquitin modification of ER associated cellular and viral proteins, alongside the actions of the 26S proteasome, are vital for the regulation of infection. Viruses can arrogate ER associated ubiquitination as well as cytosolic ubiquitin ligases with the purpose of directing the ubiquitin proteasome system (UPS to new targets. Such targets include necessary modification of viral proteins which may stabilize certain complexes, or modification of Argonaute to suppress gene silencing. The UPS machinery also contributes to the regulation of effector triggered immunity pattern recognition receptor immunity. Combining the results of unrelated studies, many positive strand RNA plant viruses appear to interact with cytosolic Ub-ligases to provide novel avenues for controlling the deleterious consequences of disease. Viral interactions with the UPS serve to regulate virus infection in a manner that promotes replication and movement, but also modulates the levels of RNA accumulation to ensure successful biotrophic interactions. In other instances, the UPS plays a central role in cellular immunity. These opposing roles are made evident by contrasting studies where knockout mutations in the UPS can either hamper viruses or lead to more aggressive diseases. Understanding how viruses manipulate ER associated post-translational machineries to better manage virus–host interactions will provide new targets for crop improvement.

Young and Especially Senescent Endothelial Microvesicles Produce NADPH: The Fuel for Their Antioxidant Machinery

Directory of Open Access Journals (Sweden)

Guillermo Bodega

2018-01-01

Full Text Available In a previous study, we demonstrated that endothelial microvesicles (eMVs have a well-developed enzymatic team involved in reactive oxygen species detoxification. In the present paper, we demonstrate that eMVs can synthesize the reducing power (NAD(PH that nourishes this enzymatic team, especially those eMVs derived from senescent human umbilical vein endothelial cells. Moreover, we have demonstrated that the molecules that nourish the enzymatic machinery involved in NAD(PH synthesis are blood plasma metabolites: lactate, pyruvate, glucose, glycerol, and branched-chain amino acids. Drastic biochemical changes are observed in senescent eMVs to optimize the synthesis of reducing power. Mitochondrial activity is diminished and the glycolytic pathway is modified to increase the activity of the pentose phosphate pathway. Different dehydrogenases involved in NADPH synthesis are also increased. Functional experiments have demonstrated that eMVs can synthesize NADPH. In addition, the existence of NADPH in eMVs was confirmed by mass spectrometry. Multiphoton confocal microscopy images corroborate the synthesis of reducing power in eMVs. In conclusion, our present and previous results demonstrate that eMVs can act as autonomous reactive oxygen species scavengers: they use blood metabolites to synthesize the NADPH that fuels their antioxidant machinery. Moreover, senescent eMVs have a stronger reactive oxygen species scavenging capacity than young eMVs.

Hormone activities and the cell cycle machinery in immunity-triggered growth inhibition.

Science.gov (United States)

Reitz, M U; Gifford, M L; Schäfer, P

2015-04-01

Biotic stress and diseases caused by pathogen attack pose threats in crop production and significantly reduce crop yields. Enhancing immunity against pathogens is therefore of outstanding importance in crop breeding. However, this must be balanced, as immune activation inhibits plant growth. This immunity-coupled growth trade-off does not support resistance but is postulated to reflect the reallocation of resources to drive immunity. There is, however, increasing evidence that growth-immunity trade-offs are based on the reconfiguration of hormone pathways, shared by growth and immunity signalling. Studies in roots revealed the role of hormones in orchestrating growth across different cell types, with some hormones showing a defined cell type-specific activity. This is apparently highly relevant for the regulation of the cell cycle machinery and might be part of the growth-immunity cross-talk. Since plants are constantly exposed to Immuno-activating microbes under agricultural conditions, the transition from a growth to an immunity operating mode can significantly reduce crop yield and can conflict our efforts to generate next-generation crops with improved yield under climate change conditions. By focusing on roots, we outline the current knowledge of hormone signalling on the cell cycle machinery to explain growth trade-offs induced by immunity. By referring to abiotic stress studies, we further introduce how root cell type-specific hormone activities might contribute to growth under immunity and discuss the feasibility of uncoupling the growth-immunity cross-talk. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation

Science.gov (United States)

Schermelleh, Lothar; Haemmer, Andrea; Spada, Fabio; Rösing, Nicole; Meilinger, Daniela; Rothbauer, Ulrich; Cardoso, M. Cristina; Leonhardt, Heinrich

2007-01-01

Postreplicative maintenance of genomic methylation patterns was proposed to depend largely on the binding of DNA methyltransferase 1 (Dnmt1) to PCNA, a core component of the replication machinery. We investigated how the slow and discontinuous DNA methylation could be mechanistically linked with fast and processive DNA replication. Using photobleaching and quantitative live cell imaging we show that Dnmt1 binding to PCNA is highly dynamic. Activity measurements of a PCNA-binding-deficient mutant with an enzyme-trapping assay in living cells showed that this interaction accounts for a 2-fold increase in methylation efficiency. Expression of this mutant in mouse dnmt1−/− embryonic stem (ES) cells restored CpG island methylation. Thus association of Dnmt1 with the replication machinery enhances methylation efficiency, but is not strictly required for maintaining global methylation. The transient nature of this interaction accommodates the different kinetics of DNA replication and methylation while contributing to faithful propagation of epigenetic information. PMID:17576694

The grouting handbook a step-by-step guide for foundation design and machinery installation

CERN Document Server

Harrison, Donald M

2013-01-01

Minimize loss of revenue and the downtime of critical assets by avoiding foundation cracking, poor bonds, and initial alignment changes. After their successful introduction as a maintenance material, machinery grouts are now being used for equipment placement in new construction. While certainly suitable for both markets and applications, a successful installation depends on proper grout selection, application, foundation preparation, and forming methods. Therefore, guidelines on their uses and limitations are needed for engineers and maintenance personnel. Based on 45 years of field experi

KDH – Kunst Design Håndværk: Et Folkeskoleforsøg

DEFF Research Database (Denmark)

Chemi, Tatiana

2009-01-01

before many other Danish schools Engum School tried in 2009 to integrate three different creative subjects (woodwork, needlecraft and visual art) to create a new subject: Design. This initiative aimed at inspire creativity and innovation in students.......before many other Danish schools Engum School tried in 2009 to integrate three different creative subjects (woodwork, needlecraft and visual art) to create a new subject: Design. This initiative aimed at inspire creativity and innovation in students....

Improved spectral kurtosis with adaptive redundant multiwavelet packet and its applications for rotating machinery fault detection

International Nuclear Information System (INIS)

Chen, Jinglong; Zi, Yanyang; He, Zhengjia; Yuan, Jing

2012-01-01

Rotating machinery fault detection is significant to avoid serious accidents and huge economic losses effectively. However, due to the vibration signal with the character of non-stationarity and nonlinearity, the detection and extraction of the fault feature turn into a challenging task. Therefore, a novel method called improved spectral kurtosis (ISK) with adaptive redundant multiwavelet packet (ARMP) is proposed for this task. Spectral kurtosis (SK) has been proved to be a powerful tool to detect and characterize the non-stationary signal. To improve the SK in filter limitation and enhance the resolution of spectral analysis as well as match fault feature optimally, the ARMP is introduced into the SK. Moreover, since kurtosis does not reflect the actual trend of periodic impulses, the SK is improved by incorporating an evaluation index called envelope spectrum entropy as supplement. The proposed method is applied to the rolling element bearing and gear fault detection to validate its reliability and effectiveness. Compared with the conventional frequency spectrum, envelope spectrum, original SK and some single wavelet methods, the results indicate that it could improve the accuracy of frequency-band selection and enhance the ability of rotating machinery fault detection. (paper)

Model of Environmental Problems Priority Arising from the use of Environmental and Natural Resources in Machinery Sectors of Thailand

Science.gov (United States)

Sutthichaimethee, Pruethsan; Sawangdee, Yothin

2016-05-01

The objective of this research is to propose an indicator to evaluate environmental impacts from the Machinery sectors of Thailand, leading to more sustainable consumption and production in this sector of the economy. The factors used to calculate the Forward Linkage, Backward Linkage and Real Benefit were the Total Environmental Costs. The highest total environmental cost was Railway Equipment which needs to be resolved immediately because it uses natural resources more than its carrying capacity, higher environmental cost than standard, and contributes low real benefit. Electric Accumulator & Battery, Secondary Special Industrial Machinery, Motorcycle, Bicycle & Other Carriages, and Engines and Turbines need to be monitored closely because they are able to link to other production sectors more than any other production sectors do, and they have high environmental cost. To decide a sustainable development strategy of the country, therefore, results of this research must be used to support decision-making.

Effects of the Occupational Safety and Health Administration's control of hazardous energy (lockout/tagout) standard on rates of machinery-related fatal occupational injury.

Science.gov (United States)

Bulzacchelli, Maria T; Vernick, Jon S; Webster, Daniel W; Lees, Peter S J

2007-10-01

To evaluate the impact of the United States' federal Occupational Safety and Health Administration's control of hazardous energy (lockout/tagout) standard on rates of machinery-related fatal occupational injury. The standard, which took effect in 1990, requires employers in certain industries to establish an energy control program and sets minimum criteria for energy control procedures, training, inspections, and hardware. An interrupted time-series design was used to determine the standard's effect on fatality rates. Machinery-related fatalities, obtained from the National Traumatic Occupational Fatalities surveillance system for 1980 through 2001, were used as a proxy for lockout/tagout-related fatalities. Linear regression was used to control for changes in demographic and economic factors. The average annual crude rate of machinery-related fatalities in manufacturing changed little from 1980 to 1989, but declined by 4.59% per year from 1990 to 2001. However, when controlling for demographic and economic factors, the regression model estimate of the standard's effect is a small, non-significant increase of 0.05 deaths per 100 000 production worker full-time equivalents (95% CI -0.14 to 0.25). When fatality rates in comparison groups that should not have been affected by the standard are incorporated into the analysis, there is still no significant change in the rate of machinery-related fatalities in manufacturing. There is no evidence that the lockout/tagout standard decreased fatality rates relative to other trends in occupational safety over the study period. A possible explanation is voluntary use of lockout/tagout by some employers before introduction of the standard and low compliance by other employers after.

Chronic clenbuterol treatment compromises force production without directly altering skeletal muscle contractile machinery

Science.gov (United States)

Py, G; Ramonatxo, C; Sirvent, P; Sanchez, A M J; Philippe, A G; Douillard, A; Galbès, O; Lionne, C; Bonnieu, A; Chopard, A; Cazorla, O; Lacampagne, A; Candau, R B

2015-01-01

Clenbuterol is a β2-adrenergic receptor agonist known to induce skeletal muscle hypertrophy and a slow-to-fast phenotypic shift. The aim of the present study was to test the effects of chronic clenbuterol treatment on contractile efficiency and explore the underlying mechanisms, i.e. the muscle contractile machinery and calcium-handling ability. Forty-three 6-week-old male Wistar rats were randomly allocated to one of six groups that were treated with either subcutaneous equimolar doses of clenbuterol (4 mg kg−1 day−1) or saline solution for 9, 14 or 21 days. In addition to the muscle hypertrophy, although an 89% increase in absolute maximal tetanic force (Po) was noted, specific maximal tetanic force (sPo) was unchanged or even depressed in the slow twitch muscle of the clenbuterol-treated rats (P muscle contraction and relaxation force kinetics indicated that clenbuterol treatment significantly reduced the rate constant of force development and the slow and fast rate constants of relaxation in extensor digitorum longus muscle (P fast rate constant of relaxation in soleus muscle (P fibres (fast twitch fibres) from clenbuterol-treated animals demonstrated decreased amplitude after 14 days (−19%, P < 0.01) and 21 days (−25%, P < 0.01). In conclusion, we showed that chronic clenbuterol treatment reduces contractile efficiency, with altered contraction and relaxation kinetics, but without directly altering the contractile machinery. Lower Ca2+ release during contraction could partially explain these deleterious effects. PMID:25656230

Optimization of advanced liquid natural gas-fuelled machineries for a high-speed ferry

DEFF Research Database (Denmark)

Tveitaskog, Kari Anne; Haglind, Fredrik

-based optimization routine are used. The top cycle is modeled as the aero-derivative gas turbine LM2500, while the following five options for bottoming cycles are modeled: ∙ Single pressure steam cycle ∙ Dual-pressure steam cycle ∙ ORC using Toluene as the working fluid with an intermediate oil loop ∙ ABC with inter......This report is aimed at designing and optimizing combined cycles in order to define the most suitable machinery system for the future high-speed Incat ferry operated by Mols-Linien. For this purpose, an in-house numerical simulation tool called DNA (Dynamic Network Analysis) and a genetic algorithm...

Towards improving the administrative machinery for health care in the Midwestern State of Nigeria.

Science.gov (United States)

Ebie, J C

1976-01-01

The paper discusses the present machinery for the administration of health care facilities in the Midwestern State of Nigeria and makes suggestions for improvement. The multiplicity of autonomous authorities involved in the running of health care facilities and the compartmentalization of health care into 'preventive' aspects (managed by the State Ministry of Health and Local Authorities) and 'curative' aspects (managed by the State Hospitals Management Board) are seen as the main disadvantages of the present system. A new administrative set-up is suggested, the highlights of which include the creation of a number of Area Health Boards that will have responsibility for all State Government and Local Authority health care facilities in their respective geographically defined areas of jurisdiction (this will abolish the artificial division between the administrations of 'preventive' and 'curative' aspects of health care), more professional divisions in the state Ministry of Health (which will retain responsibility on behalf of government for policy matters and the provision of health care facilities) than at the moment, a State Health Service Commission and A State Health Advisory Committee. It is important for doctors and other personnel in the health care field to know something about the administrative machinery of the health care delivery system in which they work. Apart from doctors who are trained in certain postgraduate fields, most other doctors do not appear to have any formal training in or early exposure to medical administration and yet, some of them get called upon during their career to undertake administrative duties at a very high level. This paper describes the present system of administration of health care facilities in the Midwestern State and offers suggestions for consideration for improvement. It is a well known fact that the administration of health care facilities in the Midwestern State has improved considerably in recent years. The

Charcot-Marie-Tooth disease-linked protein SIMPLE functions with the ESCRT machinery in endosomal trafficking

OpenAIRE

Lee, Samuel M.; Chin, Lih-Shen; Li, Lian

2012-01-01

Mutations in small integral membrane protein of lysosome/late endosome (SIMPLE) cause autosomal dominant, Charcot-Marie-Tooth disease (CMT) type 1C. The cellular function of SIMPLE is unknown and the pathogenic mechanism of SIMPLE mutations remains elusive. Here, we report that SIMPLE interacted and colocalized with endosomal sorting complex required for transport (ESCRT) components STAM1, Hrs, and TSG101 on early endosomes and functioned with the ESCRT machinery in the control of endosome-to...

An integrated multi-sensor fusion-based deep feature learning approach for rotating machinery diagnosis

Science.gov (United States)

Liu, Jie; Hu, Youmin; Wang, Yan; Wu, Bo; Fan, Jikai; Hu, Zhongxu

2018-05-01

The diagnosis of complicated fault severity problems in rotating machinery systems is an important issue that affects the productivity and quality of manufacturing processes and industrial applications. However, it usually suffers from several deficiencies. (1) A considerable degree of prior knowledge and expertise is required to not only extract and select specific features from raw sensor signals, and but also choose a suitable fusion for sensor information. (2) Traditional artificial neural networks with shallow architectures are usually adopted and they have a limited ability to learn the complex and variable operating conditions. In multi-sensor-based diagnosis applications in particular, massive high-dimensional and high-volume raw sensor signals need to be processed. In this paper, an integrated multi-sensor fusion-based deep feature learning (IMSFDFL) approach is developed to identify the fault severity in rotating machinery processes. First, traditional statistics and energy spectrum features are extracted from multiple sensors with multiple channels and combined. Then, a fused feature vector is constructed from all of the acquisition channels. Further, deep feature learning with stacked auto-encoders is used to obtain the deep features. Finally, the traditional softmax model is applied to identify the fault severity. The effectiveness of the proposed IMSFDFL approach is primarily verified by a one-stage gearbox experimental platform that uses several accelerometers under different operating conditions. This approach can identify fault severity more effectively than the traditional approaches.

Hepatitis C Virus Proteins Interact with the Endosomal Sorting Complex Required for Transport (ESCRT) Machinery via Ubiquitination To Facilitate Viral Envelopment.

Science.gov (United States)

Barouch-Bentov, Rina; Neveu, Gregory; Xiao, Fei; Beer, Melanie; Bekerman, Elena; Schor, Stanford; Campbell, Joseph; Boonyaratanakornkit, Jim; Lindenbach, Brett; Lu, Albert; Jacob, Yves; Einav, Shirit

2016-11-01

Enveloped viruses commonly utilize late-domain motifs, sometimes cooperatively with ubiquitin, to hijack the endosomal sorting complex required for transport (ESCRT) machinery for budding at the plasma membrane. However, the mechanisms underlying budding of viruses lacking defined late-domain motifs and budding into intracellular compartments are poorly characterized. Here, we map a network of hepatitis C virus (HCV) protein interactions with the ESCRT machinery using a mammalian-cell-based protein interaction screen and reveal nine novel interactions. We identify HRS (hepatocyte growth factor-regulated tyrosine kinase substrate), an ESCRT-0 complex component, as an important entry point for HCV into the ESCRT pathway and validate its interactions with the HCV nonstructural (NS) proteins NS2 and NS5A in HCV-infected cells. Infectivity assays indicate that HRS is an important factor for efficient HCV assembly. Specifically, by integrating capsid oligomerization assays, biophysical analysis of intracellular viral particles by continuous gradient centrifugations, proteolytic digestion protection, and RNase digestion protection assays, we show that HCV co-opts HRS to mediate a late assembly step, namely, envelopment. In the absence of defined late-domain motifs, K63-linked polyubiquitinated lysine residues in the HCV NS2 protein bind the HRS ubiquitin-interacting motif to facilitate assembly. Finally, ESCRT-III and VPS/VTA1 components are also recruited by HCV proteins to mediate assembly. These data uncover involvement of ESCRT proteins in intracellular budding of a virus lacking defined late-domain motifs and a novel mechanism by which HCV gains entry into the ESCRT network, with potential implications for other viruses. Viruses commonly bud at the plasma membrane by recruiting the host ESCRT machinery via conserved motifs termed late domains. The mechanism by which some viruses, such as HCV, bud intracellularly is, however, poorly characterized. Moreover, whether

The optimization model of the logging machinery usage in forestry practice

Directory of Open Access Journals (Sweden)

Jitka Janová

2009-01-01

Full Text Available The decision support systems commonly used in industry and economy managerial practice for optimizing the processes are based on algoritmization of the typical decision problems. In Czech forestry business, there is a lack of developed decision support systems, which could be easily used in daily practice. This stems from the fact, that the application of optimization methods is less successful in forestry decision making than in industry or economy due to inherent complexity of the forestry decision problems. There is worldwide ongoing research on optimization models applicable in forestry decision making, but the results are not globally applicable and moreover the cost of possibly arising software tools are indispensable. Especially small and medium forestry companies in Czech Republic can not afford such additional costs, although the results of optimization could positively in­fluen­ce not only the business itself but also the impact of forestry business on the environment. Hence there is a need for user friendly optimization models for forestry decision making in the area of Czech Republic, which could be easily solved in commonly available software, and whose results would be both, realistic and easily applicable in the daily decision making.The aim of this paper is to develop the optimization model for the machinery use planning in Czech logging firm in such a way, that the results can be obtained using MS EXCEL. The goal is to identify the integer number of particular machines which should be outsourced for the next period, when the total cost minimization is required. The linear programming model is designed covering the typical restrictions on available machinery and total volume of trees to be cut and transported. The model offers additional result in the form of optimal employment of particular machines. The solution procedure is described in detail and the results obtained are discussed with respect to its applicability in

Compression-absorption (resorption) refrigerating machinery. Modeling of reactors; Machine frigorifique a compression-absorption (resorption). Modelisation des reacteurs

Energy Technology Data Exchange (ETDEWEB)

Lottin, O; Feidt, M; Benelmir, R [LEMTA-UHP Nancy-1, 54 - Vandoeuvre-les-Nancy (France)

1998-12-31

This paper is a series of transparencies presenting a comparative study of the thermal performances of different types of refrigerating machineries: di-thermal with vapor compression, tri-thermal with moto-compressor, with ejector, with free piston, adsorption-type, resorption-type, absorption-type, compression-absorption-type. A prototype of ammonia-water compression-absorption heat pump is presented and modeled. (J.S.)

Compression-absorption (resorption) refrigerating machinery. Modeling of reactors; Machine frigorifique a compression-absorption (resorption). Modelisation des reacteurs

Energy Technology Data Exchange (ETDEWEB)

Lottin, O.; Feidt, M.; Benelmir, R. [LEMTA-UHP Nancy-1, 54 - Vandoeuvre-les-Nancy (France)

1997-12-31

This paper is a series of transparencies presenting a comparative study of the thermal performances of different types of refrigerating machineries: di-thermal with vapor compression, tri-thermal with moto-compressor, with ejector, with free piston, adsorption-type, resorption-type, absorption-type, compression-absorption-type. A prototype of ammonia-water compression-absorption heat pump is presented and modeled. (J.S.)

Development and demonstration of surveillance and diagnostics of rotating machinery for reducing radiation exposure to nuclear power plant personnel: Final report

International Nuclear Information System (INIS)

Allen, J.W.; Bohanick, J.S.

1988-01-01

This program was designed to reduce radiation exposure to power plant personnel resulting from inspection, maintenance, and repair of rotating equipment. The new rotating machinery monitoring system for this program was installed at GGNS during August 1983. This document provides a functional description of the hardware and software that comprise the system and discusses the application of the monitoring system to achieving overall program goals. The analyses of the monitored rotating machinery during the plant startup phase and after the plant's first fuel cycle are presented in addition to the radiation dose reduction which occurred as a direct and indirect result of the RMSS. The dose reduction program at GGNS is reviewed and recommendations made to incorporate this program with the RMSS. 28 refs., 64 figs., 43 tabs

SKODA Nuclear Machinery - tradition and expertise in nuclear power industry

International Nuclear Information System (INIS)

Svitak, F.

1997-01-01

The SKODA Nuclear Machinery company is a major manufacturer of nuclear reactor assemblies and supplier of WWER type primary coolant circuits. In the past, the company was nearly a monopolistic manufacturer of WWER reactor assemblies supplied to the Central and East European countries (except the USSR) grouped in the former Council of Mutual Economic Assistance. Over the 1980-1993 period, 21 units of the WWER-440 type and 3 units of the WWER-1000 type were manufactured. The company keeps abreast of technological progress and has been switching to new manufacturing areas, such as compact storage racks for spent fuel pools, hermetic cable bushings, spent fuel storage and transport casks, and cooperation in the manufacture of neutron flux measuring channels. Technological services provided to nuclear power plants constitute another important field of the company's business. The company's combined expertise in Soviet and Western designed PWRs is a considerable asset. (P.A.)

Model of Environmental Problems Priority Arising from the use of Environmental and Natural Resources in Machinery Sectors of Thailand

Directory of Open Access Journals (Sweden)

Sutthichaimethee Pruethsan

2016-05-01

Full Text Available The objective of this research is to propose an indicator to evaluate environmental impacts from the Machinery sectors of Thailand, leading to more sustainable consumption and production in this sector of the economy. The factors used to calculate the Forward Linkage, Backward Linkage and Real Benefit were the Total Environmental Costs. The highest total environmental cost was Railway Equipment which needs to be resolved immediately because it uses natural resources more than its carrying capacity, higher environmental cost than standard, and contributes low real benefit. Electric Accumulator & Battery, Secondary Special Industrial Machinery, Motorcycle, Bicycle & Other Carriages, and Engines and Turbines need to be monitored closely because they are able to link to other production sectors more than any other production sectors do, and they have high environmental cost. To decide a sustainable development strategy of the country, therefore, results of this research must be used to support decision-making.

A novel component of the mitochondrial genome segregation machinery in trypanosomes

Directory of Open Access Journals (Sweden)

Anneliese Hoffmann

2016-07-01

Full Text Available We recently described a new component (TAC102 of the mitochondrial genome segregation machinery (mtGSM in the protozoan parasite Trypanosoma brucei. T. brucei belongs to a group of organisms that contain a single mitochondrial organelle with a single mitochondrial genome (mt-genome per cell. The mt-genome consists of 5000 minicircles (1 kb and 25 maxicircles (23 kb that are catenated into a large network. After replication of the network its segregation is driven by the separating basal bodies, which are homologous structures to the centrioles organizing the spindle apparatus in many eukaryotes. The structure connecting the basal body to the mt-genome was named the Tripartite Attachment Complex (TAC owing its name to the distribution across three areas in the cell including the two mitochondrial membranes.

Improvement of finishing antifriction treatment without abrasive of the rubbing parts surfaces of agricultural machineries

Directory of Open Access Journals (Sweden)

I.V. Shepelenk

2014-06-01

Full Text Available The wear of machines and mechanisms after rubbing is a major concern. The costs of manufacturing and restoration parts for agricultural machinery are enormous such as the trunnions pinions of hydraulic pumps. Finishing treatment antifriction without abrasive (FTAA is the existing method of manufacturing and restoration, but it has disadvantages like low work efficiency and the frequent replacement of the instrument. That is why a new method of FTAA parts type ''tree'' has been developed called vibratory finishing treatment antifriction without abrasive, (VFTAA method. The study was conducted at the laboratory of the State Technical University of Kirovograd (Ukraine where turn 16K20, the profilograph-profilometer "Talysurf-5", the scanning electron microscope REM-106I, friction machine MI-1M and the stand KI-28097-02M were used respectively for machining, study of micro relief before and after the VFTAA, microstructure, wear resistance and the determination of the break-in period of parts. The results showed that the VFTAA helped reduce the roughness Ra of the samples studied by half compared to the samples processed by polishing and 1.3 times compared to those treated with the FTAA, the break-in period has been reduced four times , this leads to an increase in the life of the hydraulic pump. This technology can be recommended for manufacturing and repair of hydraulic units of agricultural machineries.

New technologies in Islamic countries. Power engineering, transport, oil industry, machinery construction, building construction and information technologies problems

International Nuclear Information System (INIS)

Sharipova, N.S.

1999-01-01

The published proceedings contain brief presentations concerning new technologies in power engineering, transport, oil industry, machinery construction, building construction and information technologies presented to the International scientific and technical conference: New technologies in Islamic countries, which was organized within frame work of 6 General Assembly of Federation of engineering Institutes of Islamic countries (FEIIC). (author)

Defective HLA class I antigen processing machinery in cancer.

Science.gov (United States)

Cai, Lei; Michelakos, Theodoros; Yamada, Teppei; Fan, Song; Wang, Xinhui; Schwab, Joseph H; Ferrone, Cristina R; Ferrone, Soldano

2018-02-27

Malignant transformation of cells is frequently associated with defective HLA class I antigen processing machinery (APM) component expression. This abnormality may have functional relevance, since it may have a negative impact on tumor cell recognition by cognate T cells. Furthermore, HLA class I APM abnormalities appear to have clinical significance, since they are associated with poor prognosis in several malignant diseases and may play a role in the resistance to immune checkpoint inhibitor-based immunotherapy. In this paper, we have reviewed the literature describing abnormalities in HLA class I APM component expression in many types of cancer. These abnormalities have been reported in all types of cancer analyzed with a frequency ranging between a minimum of 35.8% in renal cancer and a maximum of 87.9% in thyroid cancer for HLA class I heavy chains. In addition, we have described the molecular mechanisms underlying defects in HLA class I APM component expression and function by malignant cells. Lastly, we have discussed the clinical significance of HLA class I APM component abnormalities in malignant tumors.

Structure, Function and Regulation of the Hsp90 Machinery

Directory of Open Access Journals (Sweden)

Jing Li

2013-06-01

Full Text Available Heat shock protein 90 (Hsp90 is an ATP-dependent molecular chaperone which is essential in eukaryotes. It is required for the activation and stabilization of a wide variety of client proteins and many of them are involved in important cellular pathways. Since Hsp90 affects numerous physiological processes such as signal transduction, intracellular transport, and protein degradation, it became an interesting target for cancer therapy. Structurally, Hsp90 is a flexible dimeric protein composed of three different domains which adopt structurally distinct conformations. ATP binding triggers directionality in these conformational changes and leads to a more compact state. To achieve its function, Hsp90 works together with a large group of cofactors, termed co-chaperones. Co-chaperones form defined binary or ternary complexes with Hsp90, which facilitate the maturation of client proteins. In addition, posttranslational modifications of Hsp90, such as phosphorylation and acetylation, provide another level of regulation. They influence the conformational cycle, co-chaperone interaction, and inter-domain communications. In this review, we discuss the recent progress made in understanding the Hsp90 machinery.

Deep Fault Recognizer: An Integrated Model to Denoise and Extract Features for Fault Diagnosis in Rotating Machinery

Directory of Open Access Journals (Sweden)

Xiaojie Guo

2016-12-01

Full Text Available Fault diagnosis in rotating machinery is significant to avoid serious accidents; thus, an accurate and timely diagnosis method is necessary. With the breakthrough in deep learning algorithm, some intelligent methods, such as deep belief network (DBN and deep convolution neural network (DCNN, have been developed with satisfactory performances to conduct machinery fault diagnosis. However, only a few of these methods consider properly dealing with noises that exist in practical situations and the denoising methods are in need of extensive professional experiences. Accordingly, rethinking the fault diagnosis method based on deep architectures is essential. Hence, this study proposes an automatic denoising and feature extraction method that inherently considers spatial and temporal correlations. In this study, an integrated deep fault recognizer model based on the stacked denoising autoencoder (SDAE is applied to both denoise random noises in the raw signals and represent fault features in fault pattern diagnosis for both bearing rolling fault and gearbox fault, and trained in a greedy layer-wise fashion. Finally, the experimental validation demonstrates that the proposed method has better diagnosis accuracy than DBN, particularly in the existing situation of noises with superiority of approximately 7% in fault diagnosis accuracy.

Comparative genomics of phylogenetically diverse unicellular eukaryotes provide new insights into the genetic basis for the evolution of the programmed cell death machinery.

Science.gov (United States)

Nedelcu, Aurora M

2009-03-01

Programmed cell death (PCD) represents a significant component of normal growth and development in multicellular organisms. Recently, PCD-like processes have been reported in single-celled eukaryotes, implying that some components of the PCD machinery existed early in eukaryotic evolution. This study provides a comparative analysis of PCD-related sequences across more than 50 unicellular genera from four eukaryotic supergroups: Unikonts, Excavata, Chromalveolata, and Plantae. A complex set of PCD-related sequences that correspond to domains or proteins associated with all main functional classes--from ligands and receptors to executors of PCD--was found in many unicellular lineages. Several PCD domains and proteins previously thought to be restricted to animals or land plants are also present in unicellular species. Noteworthy, the yeast, Saccharomyces cerevisiae--used as an experimental model system for PCD research, has a rather reduced set of PCD-related sequences relative to other unicellular species. The phylogenetic distribution of the PCD-related sequences identified in unicellular lineages suggests that the genetic basis for the evolution of the complex PCD machinery present in extant multicellular lineages has been established early in the evolution of eukaryotes. The shaping of the PCD machinery in multicellular lineages involved the duplication, co-option, recruitment, and shuffling of domains already present in their unicellular ancestors.

New technologies in Islamic countries. Power engineering, transport, oil industry, machinery construction, building construction and information technologies problems

International Nuclear Information System (INIS)

Sharipova, N.S.

1999-01-01

This issue contains papers, which reflect the most important achievements of new technologies in power engineering, transport, oil industry, machinery construction, building construction and information technologies presented to the International Scientific and Technical Conference: New technologies in Islamic countries, which was organized within frame work of 6 General Assembly of Federation of Engineering Institutes of Islamic Countries (FEIIC). (author)

Small machinery for pumping and compressing helium near 40K

International Nuclear Information System (INIS)

Swift, W.L.; Sixsmith, H.

1984-01-01

There is a significant need for small, reliable pumps and compressors suitable for circulating helium at temperatures near 4 0 K. Most pumps or compressors which have been developed to data for these applications are designed for relatively limited use. They are generally used in laboratory environments where life requirements for the experiments may be relatively short, being of the order of several hundred hours. In recent years, several applications have been identified where pumps or compressors (at liquid helium temperatures) must have high reliability if they are to be used successfully. These applications include liquid helium circulation systems through superconducting magnets and transmission lines, and cold compression applications where the compressor is used as a vacuum pump to lower the temperature of a liquid helium bath. This paper discusses the technical considerations which must be taken into account in the design and development of machinery to meet these needs. The design of a centrifugal machine which can act as a helium pump or compressor is presented

Radiation survey of aircraft and heavy machinery scrap.

Science.gov (United States)

Idriss, Hajo; Salih, Isam; Gumaa, Elsadig; Yassin, Abbas; Yousif, E H; Abdel Hamid, Saad Eldeen M; Sam, A K

2012-12-01

This study was conducted primarily to survey aircraft and heavy machinery at 30 locations within Khartoum State using handheld radiation survey meters to detect and identify any radiation sources that might be present and to estimate radiation dose levels. The survey has resulted in detection of 16 sealed sources of (90)Sr and one of (226)Ra in aircraft scrap. Of course, (90)Sr sources are used in military aircraft as temperature sensors while (226)Ra is used for indicating fuel levels. These sources were found intact without spreading radioactivity contamination; however, none was detected in heavy machine scrap. The levels of radiation dose measured at 0.1m from the source fall within the range of 25.1-40.2 μSv/h with an average value of 33.52 ± 4.06 μSv/h. These orphan sources have been separated from the scrap, tested for possible leakage, conditioned and stored in waste management facility. The result of this study has revealed without doubt that the scrap constitute a serious source of public exposure and highlights the importance of legislation making radiation monitoring of scrap in the country mandatory before it is sold to metal industry for reprocessing. Copyright © 2012 Elsevier Ltd. All rights reserved.

Exploiting immune cell metabolic machinery for functional HIV cure and the prevention of inflammaging.

Science.gov (United States)

Palmer, Clovis S; Palchaudhuri, Riya; Albargy, Hassan; Abdel-Mohsen, Mohamed; Crowe, Suzanne M

2018-01-01

An emerging paradigm in immunology suggests that metabolic reprogramming and immune cell activation and functions are intricately linked. Viral infections, such as HIV infection, as well as cancer force immune cells to undergo major metabolic challenges. Cells must divert energy resources in order to mount an effective immune response. However, the fact that immune cells adopt specific metabolic programs to provide host defense against intracellular pathogens and how this metabolic shift impacts immune cell functions and the natural course of diseases have only recently been appreciated. A clearer insight into how these processes are inter-related will affect our understanding of several fundamental aspects of HIV persistence. Even in patients with long-term use of anti-retroviral therapies, HIV infection persists and continues to cause chronic immune activation and inflammation, ongoing and cumulative damage to multiple organs systems, and a reduction in life expectancy. HIV-associated fundamental changes to the metabolic machinery of the immune system can promote a state of "inflammaging", a chronic, low-grade inflammation with specific immune changes that characterize aging, and can also contribute to the persistence of HIV in its reservoirs. In this commentary, we will bring into focus evolving concepts on how HIV modulates the metabolic machinery of immune cells in order to persist in reservoirs and how metabolic reprogramming facilitates a chronic state of inflammation that underlies the development of age-related comorbidities. We will discuss how immunometabolism is facilitating the changing paradigms in HIV cure research and outline the novel therapeutic opportunities for preventing inflammaging and premature development of age-related conditions in HIV + individuals.

Arabidopsis mRNA polyadenylation machinery: comprehensive analysis of protein-protein interactions and gene expression profiling

Directory of Open Access Journals (Sweden)

Mo Min

2008-05-01

Full Text Available Abstract Background The polyadenylation of mRNA is one of the critical processing steps during expression of almost all eukaryotic genes. It is tightly integrated with transcription, particularly its termination, as well as other RNA processing events, i.e. capping and splicing. The poly(A tail protects the mRNA from unregulated degradation, and it is required for nuclear export and translation initiation. In recent years, it has been demonstrated that the polyadenylation process is also involved in the regulation of gene expression. The polyadenylation process requires two components, the cis-elements on the mRNA and a group of protein factors that recognize the cis-elements and produce the poly(A tail. Here we report a comprehensive pairwise protein-protein interaction mapping and gene expression profiling of the mRNA polyadenylation protein machinery in Arabidopsis. Results By protein sequence homology search using human and yeast polyadenylation factors, we identified 28 proteins that may be components of Arabidopsis polyadenylation machinery. To elucidate the protein network and their functions, we first tested their protein-protein interaction profiles. Out of 320 pair-wise protein-protein interaction assays done using the yeast two-hybrid system, 56 (~17% showed positive interactions. 15 of these interactions were further tested, and all were confirmed by co-immunoprecipitation and/or in vitro co-purification. These interactions organize into three distinct hubs involving the Arabidopsis polyadenylation factors. These hubs are centered around AtCPSF100, AtCLPS, and AtFIPS. The first two are similar to complexes seen in mammals, while the third one stands out as unique to plants. When comparing the gene expression profiles extracted from publicly available microarray datasets, some of the polyadenylation related genes showed tissue-specific expression, suggestive of potential different polyadenylation complex configurations. Conclusion An

The U.S. Department of Labor's Tractor and Machinery Certification Program: management styles and perceptions held by community stakeholders and instructors.

Science.gov (United States)

Jepsen, S D

2012-07-01

A mixed-mode, descriptive study was conducted on the U.S. Department of Labor's (DOL) Tractor and Machinery Certification Program. Legislated by the Fair Labors Standards Act, the Hazardous Occupations Order in Agriculture (HOOA) was enacted in 1968 as a public policy measure to reduce the number of injuries to youth on farms. An educational exemption allows youth 14 and 15 years of age to work for hire after they successfully complete a training program. In the 40+ years since the legislation went into effect prescribing such training exemptions, deficiencies and variations have occurred in the quality of the educational program and the system by which agencies certify young people. To gain a better understanding of the DOL Tractor and Machinery Certification program, community stakeholders were asked to identify management practices, curriculum resources, and perceptions of the DOL program. The study design used qualitative data from 49 agricultural representatives participating in regional focus groups and quantitative data from 330 community instructors responding to an electronic-formatted questionnaire (representing a 70.7% response rate) in an effort to answer the question: "What is the current status of the DOL Tractor and Machinery Certification program in the United States?" The findings revealed that 55.2% of the instructors taught a qualifying DOL program. Of these, the certification was administered through an Extension or 4-H program (68.7%), an agricultural education program (24.7%), or as a combination of Extension and agricultural education (6.0%). Course instructors believed the training was beneficial to students and had opportunity to attract more students than currently enrolled These instructors placed a higher value on standardized teaching materials than on standardized testing procedures; they also supported the need for additional teaching aids, which included hands-on activities, videotapes, student workbooks, and DVDs. Study participants

A comparison study of support vector machines and hidden Markov models in machinery condition monitoring

International Nuclear Information System (INIS)

Miao, Qiang; Huang, Hong Zhong; Fan, Xianfeng

2007-01-01

Condition classification is an important step in machinery fault detection, which is a problem of pattern recognition. Currently, there are a lot of techniques in this area and the purpose of this paper is to investigate two popular recognition techniques, namely hidden Markov model and support vector machine. At the beginning, we briefly introduced the procedure of feature extraction and the theoretical background of this paper. The comparison experiment was conducted for gearbox fault detection and the analysis results from this work showed that support vector machine has better classification performance in this area

Transcriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reduction.

Science.gov (United States)

Tetteh, Antonia Y; Sun, Katherine H; Hung, Chiu-Yueh; Kittur, Farooqahmed S; Ibeanu, Gordon C; Williams, Daniel; Xie, Jiahua

2014-01-01

Bacteria can reduce toxic selenite into less toxic, elemental selenium (Se(0)), but the mechanism on how bacterial cells reduce selenite at molecular level is still not clear. We used Escherichia coli strain K12, a common bacterial strain, as a model to study its growth response to sodium selenite (Na2SeO3) treatment and then used quantitative real-time PCR (qRT-PCR) to quantify transcript levels of three E. coli selenopolypeptide genes and a set of machinery genes for selenocysteine (SeCys) biosynthesis and incorporation into polypeptides, whose involvements in the selenite reduction are largely unknown. We determined that 5 mM Na2SeO3 treatment inhibited growth by ∼ 50% while 0.001 to 0.01 mM treatments stimulated cell growth by ∼ 30%. Under 50% inhibitory or 30% stimulatory Na2SeO3 concentration, selenopolypeptide genes (fdnG, fdoG, and fdhF) whose products require SeCys but not SeCys biosynthesis machinery genes were found to be induced ≥2-fold. In addition, one sulfur (S) metabolic gene iscS and two previously reported selenite-responsive genes sodA and gutS were also induced ≥2-fold under 50% inhibitory concentration. Our findings provide insight about the detoxification of selenite in E. coli via induction of these genes involved in the selenite reduction process.

Developmental Programming: Impact of Prenatal Testosterone Excess on Steroidal Machinery and Cell Differentiation Markers in Visceral Adipocytes of Female Sheep.

Science.gov (United States)

Puttabyatappa, Muraly; Lu, Chunxia; Martin, Jacob D; Chazenbalk, Gregorio; Dumesic, Daniel; Padmanabhan, Vasantha

2017-01-01

Prenatal testosterone (T)-treated female sheep manifest reduced adipocyte size and peripheral insulin resistance. The small adipocyte phenotype may reflect defects in adipogenesis and its steroidal machinery. To test whether prenatal T treatment from gestational days 30 to 90 alters the visceral adipose tissue (VAT) steroidal machinery and reduces adipocyte differentiation, we examined expression of the steroidogenic enzymes, steroid receptors, and adipocyte differentiation markers at fetal day 90 and postnatal ages 10 and 21 months. Because gestational T treatment increases fetal T and maternal insulin, the contributions of these were assessed by androgen receptor antagonist or insulin sensitizer cotreatment, either separately (at fetal day 90 and 21 months of age time points) or together (10 months of age). The effects on adipogenesis were assessed in the VAT-derived mesenchymal stem cells (AT-MSCs) from pre- and postpubertal time points to evaluate the effects of pubertal steroidal changes on adipogenesis. Our results show that VAT manifests potentially a predominant estrogenic intracrine milieu (increased aromatase and estrogen receptor α) and reduced differentiation markers at fetal day 90 and postnatal 21 months of age. These changes appear to involve both androgenic and metabolic pathways. Preliminary findings suggest that prenatal T treatment reduces adipogenesis, decreases expression of differentiation, and increases expression of commitment markers at both pre- and postpubertal time points. Together, these findings suggest that (1) increased commitment of AT-MSCs to adipocyte lineage and decreased differentiation to adipocytes may underlie the small adipocyte phenotype of prenatal T-treated females and (2) excess T-induced changes in steroidal machinery in the VAT likely participate in the programming/maintenance of this defect.

Automation facilities for agricultural machinery control

Directory of Open Access Journals (Sweden)

A. Yu. Izmaylov

2017-01-01

Full Text Available The possibility of use of the automation equipment for agricultural machinery control is investigated. The authors proposed solutions on creation of the centralized unified automated information system for mobile aggregates management. In accordance with the modern requirements this system should be open, integrated into the general schema of agricultural enterprise control. Standard hardware, software and communicative features should be realized in tasks of monitoring and control. Therefore the schema should be get with use the unified modules and Russian standards. The complex multivariate unified automated control system for different objects of agricultural purpose based on block and modular creation should correspond to the following principles: high reliability, simplicity of service, low expenses in case of operation, the short payback period connected to increase in productivity, the reduced losses when harvesting, postharvest processing and storage, the improved energetic indices. Technological processes control in agricultural production is exercised generally with feedback. The example without feedback is program control by temperature in storage in case of the cooling mode. Feedback at technological processes control in agricultural production allows to optimally solve a problem of rational distribution of functions in man-distributed systems and forming the intelligent ergonomic interfaces, consistent with professional perceptions of decision-makers. The negative feedback created by the control unit allows to support automatically a quality index of technological process at the set level. The quantitative analysis of a production situation base itself upon deeply formalized basis of computer facilities that promotes making of the optimal solution. Information automated control system introduction increases labor productivity by 40 percent, reduces energetic costs by 25 percent. Improvement of quality of the executed technological

Mobile-Dose: A Dose-Meter Designed for Use in Automatic Machineries for Dose Manipulation in Nuclear Medicine

Science.gov (United States)

de Asmundis, Riccardo; Boiano, Alfonso; Ramaglia, Antonio

2008-06-01

Mobile-Dose has been designed for a very innovative use: the integration in a robotic machinery for automatic preparation of radioactive doses, to be injected to patients in Nuclear Medicine Departments, with real time measurement of the activity under preparation. Mobile-Dose gives a constant measurement of the dose during the filling of vials or syringes, triggering the end of the filling process based on a predefined dose limit. Several applications of Mobile-Dose have been delivered worldwide, from Italian hospitals and clinics to European and Japanese ones. The design of such an instrument and its integration in robotic machineries, was required by an Italian company specialised in radiation protection tools for nuclear applications, in the period 2001-2003. At the time of its design, apparently no commercial instruments with a suitable interfacing capability to the external world existed: we designed it in order to satisfy all the strict requirements coming from the medical aspects (precision within 10%, repeatability, stability, time response) and from the industrial conceiving principles that are mandatory to ensure a good reliability in such a complicated environment. The instrument is suitable to be used in standalone mode too, thanks to its portability and compactness and to the intelligent operator panel programmed for this purpose.

Damage detection in rotating machinery by means of entropy-based parameters

Science.gov (United States)

Tocarciuc, Alexandru; Bereteu, Liviu; ǎgǎnescu, Gheorghe Eugen, Dr

2014-11-01

The paper is proposing two new entropy-based parameters, namely Renyi Entropy Index (REI) and Sharma-Mittal Entropy Index (SMEI), for detecting the presence of failures (or damages) in rotating machinery, namely: belt structural damage, belt wheels misalignment, failure of the fixing bolt of the machine to its baseplate and eccentricities (i.e.: due to detaching a small piece of material or bad mounting of the rotating components of the machine). The algorithms to obtain the proposed entropy-based parameters are described and test data is used in order to assess their sensitivity. A vibration test bench is used for measuring the levels of vibration while artificially inducing damage. The deviation of the two entropy-based parameters is compared in two states of the vibration test bench: not damaged and damaged. At the end of the study, their sensitivity is compared to Shannon Entropic Index.

木材切削工具の切れ味測定と切れ味評価-11 : 改良鉋の切れ味-1 : 各種改良鉋の新調時における切れ味

OpenAIRE

杉山, 滋

1991-01-01

The invention of the Japanese hand plane with a cap iron was the most important advance in the history of woodworking tools in the last one hundred years. It has seemingly a very simple mechanism, but it demands a lot of skill to adjust the each part of the plane. Therefore, it is not suitable for a general amateur and an educational use. The Japanese hand plane is one of the basic tools used by junior high school students for woodworking, and several kinds of improved hand planes designed fo...

木材切削工具の切れ味測定と切れ味評価-12 : 改良鉋の切れ味-2 : 調整後の初期鉋削段階における切れ味

OpenAIRE

杉山, 滋

1992-01-01

The invention of the Japanese hand plane with a cap iron was the most important advance in the history of woodworking tools in the last one hundred years. It has seemingly a very simple mechanism, but it demands a lot of skill to adjust the each part of the plane. Therefore, it is not suitable for a general amateur and an educational use. The Japanese hand plane is one of the basic tools used by junior high school students for woodworking, and several kinds of improved hand planes designed fo...

Machinery penile injuries associated with traditional trousers of the Yoruba of South-western Nigeria: A consideration for proper work clothes

Directory of Open Access Journals (Sweden)

A.A. Popoola

2012-03-01

Full Text Available Appropriateness of garments for different vocations to avoid unintended injury to the worker has always been recognized. This report of two cases of machinery penile injuries in patients wearing the traditional trousers of the Yoruba tribe of south-western Nigeria emphasizes the need for wearing appropriate clothes when operating machines.

The Endoplasmic Reticulum Coat Protein II Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis*

Science.gov (United States)

Fryer, Lee G. D.; Jones, Bethan; Duncan, Emma J.; Hutchison, Claire E.; Ozkan, Tozen; Williams, Paul A.; Alder, Olivia; Nieuwdorp, Max; Townley, Anna K.; Mensenkamp, Arjen R.; Stephens, David J.; Dallinga-Thie, Geesje M.; Shoulders, Carol C.

2014-01-01

Triglycerides and cholesterol are essential for life in most organisms. Triglycerides serve as the principal energy storage depot and, where vascular systems exist, as a means of energy transport. Cholesterol is essential for the functional integrity of all cellular membrane systems. The endoplasmic reticulum is the site of secretory lipoprotein production and de novo cholesterol synthesis, yet little is known about how these activities are coordinated with each other or with the activity of the COPII machinery, which transports endoplasmic reticulum cargo to the Golgi. The Sar1B component of this machinery is mutated in chylomicron retention disorder, indicating that this Sar1 isoform secures delivery of dietary lipids into the circulation. However, it is not known why some patients with chylomicron retention disorder develop hepatic steatosis, despite impaired intestinal fat malabsorption, and why very severe hypocholesterolemia develops in this condition. Here, we show that Sar1B also promotes hepatic apolipoprotein (apo) B lipoprotein secretion and that this promoting activity is coordinated with the processes regulating apoB expression and the transfer of triglycerides/cholesterol moieties onto this large lipid transport protein. We also show that although Sar1A antagonizes the lipoprotein secretion-promoting activity of Sar1B, both isoforms modulate the expression of genes encoding cholesterol biosynthetic enzymes and the synthesis of cholesterol de novo. These results not only establish that Sar1B promotes the secretion of hepatic lipids but also adds regulation of cholesterol synthesis to Sar1B's repertoire of transport functions. PMID:24338480

The Resource Benefits Evaluation Model on Remanufacturing Processes of End-of-Life Construction Machinery under the Uncertainty in Recycling Price

Directory of Open Access Journals (Sweden)

Qian-wang Deng

2017-02-01

Full Text Available In the process of end-of-life construction machinery remanufacturing, the existence of uncertainties in all aspects of the remanufacturing process increase the difficulty and complexity of resource benefits evaluation for them. To quantify the effects of those uncertainty factors, this paper makes a mathematical analysis of the recycling and remanufacturing processes, building a resource benefits evaluation model for the end-of-life construction machinery. The recycling price and the profits of remanufacturers can thereby be obtained with a maximum remanufacturing resource benefit. The study investigates the change regularity of the resource benefits, recycling price, and profits of remanufacturers when the recycling price, quality fluctuation coefficient, demand coefficient, and the reusing ratio of products or parts are varying. In the numerical experiment, we explore the effects of uncertainties on the remanufacturing decisions and the total expected costs. The simulated analysis shows when the quality fluctuation coefficient is approaching to 1, the values of the profits of remanufacturer, the maximal resource benefits and recycling price grade into constants.

Injury/Fatality-Causing Incidents Involving the Rearward Movement of Agricultural Machinery: Types, Causes, and Preventive Measures

Directory of Open Access Journals (Sweden)

Shawn G. Ehlers

2017-02-01

Full Text Available The research reported here sought to more fully understand the types and causative factors of injury/fatality incidents resulting from the rearward-movement of tractors and other self-propelled agricultural machinery, with the view that such findings might lead to the development, improvement, and/or better utilization of safety procedures, design principles, and technologies that would prevent—or at least markedly reduce—their occurrence. Thus, the scope of this study focused only on rearward-travel (not mechanical malfunction incidents, and principally on agricultural equipment (although cases involving similar equipment in industrial or construction settings were also drawn upon. Applying these two criteria, a search of published and online sources uncovered more than 100 documented cases, 35 of which could clearly be identified as rearward-movement incidents, of which 28 (80% were fatal. Each of these 35 cases were then assessed, based on the type of machine, type of worksite, and type/description of incident (i.e., ‘scenario’, which fell into one of three distinct categories or classifications—(1 co-worker run over/crushed/otherwise injured because operator loses visual contact with co-worker; (2 bystander run over/crushed/otherwise injured because operator is unaware of bystander’s presence; and (3 operator run over/crushed/otherwise injured because operator loses visual contact with, or is unaware of, a stationary object or a hazard. Then, from each scenario, a representative incident (i.e., case study was selected for a more in-depth analysis. The collective findings, from these three case studies and all 35 machinery rearward-movement incidents, were as follows: (1 The ‘victim’ could be the machine operator as well as a co-worker or a bystander; (2 The specific site of the co-worker or bystander injury/fatality was at the base of the machine’s rear tires or tracks, at the hitching point, or behind a towed implement; (3

Performance of refrigerating machineries with new refrigerants; Performance des machines frigorifiques avec les nouveaux refrigerants

Energy Technology Data Exchange (ETDEWEB)

Bailly, A; Jurkowski, R [CIAT, 01 - Culoz (France)

1998-12-31

This paper reports on a comparative study of the thermal performances of different refrigerants like R-22, R-134a, R-404A and R-407C when used as possible substitutes for the HCFC22 refrigerant in a given refrigerating machinery equipped with compact high performance plate exchangers. Thermal performances are compared in identical operating conditions. The behaviour of the two-phase exchange coefficient is analyzed with respect to the different parameters. The composition of the mixture after one year of operation has been analyzed too and the influence of oil on the performances is studied. (J.S.)

Performance of refrigerating machineries with new refrigerants; Performance des machines frigorifiques avec les nouveaux refrigerants

Energy Technology Data Exchange (ETDEWEB)

Bailly, A.; Jurkowski, R. [CIAT, 01 - Culoz (France)

1997-12-31

This paper reports on a comparative study of the thermal performances of different refrigerants like R-22, R-134a, R-404A and R-407C when used as possible substitutes for the HCFC22 refrigerant in a given refrigerating machinery equipped with compact high performance plate exchangers. Thermal performances are compared in identical operating conditions. The behaviour of the two-phase exchange coefficient is analyzed with respect to the different parameters. The composition of the mixture after one year of operation has been analyzed too and the influence of oil on the performances is studied. (J.S.)

Transcriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reduction

Directory of Open Access Journals (Sweden)

Antonia Y. Tetteh

2014-01-01

Full Text Available Bacteria can reduce toxic selenite into less toxic, elemental selenium (Se0, but the mechanism on how bacterial cells reduce selenite at molecular level is still not clear. We used Escherichia coli strain K12, a common bacterial strain, as a model to study its growth response to sodium selenite (Na2SeO3 treatment and then used quantitative real-time PCR (qRT-PCR to quantify transcript levels of three E. coli selenopolypeptide genes and a set of machinery genes for selenocysteine (SeCys biosynthesis and incorporation into polypeptides, whose involvements in the selenite reduction are largely unknown. We determined that 5 mM Na2SeO3 treatment inhibited growth by ∼50% while 0.001 to 0.01 mM treatments stimulated cell growth by ∼30%. Under 50% inhibitory or 30% stimulatory Na2SeO3 concentration, selenopolypeptide genes (fdnG, fdoG, and fdhF whose products require SeCys but not SeCys biosynthesis machinery genes were found to be induced ≥2-fold. In addition, one sulfur (S metabolic gene iscS and two previously reported selenite-responsive genes sodA and gutS were also induced ≥2-fold under 50% inhibitory concentration. Our findings provide insight about the detoxification of selenite in E. coli via induction of these genes involved in the selenite reduction process.

Role of microRNAs and microRNA machinery in the pathogenesis of diffuse large B-cell lymphoma

International Nuclear Information System (INIS)

Caramuta, S; Lee, L; Özata, D M; Akçakaya, P; Georgii-Hemming, P; Xie, H; Amini, R-M; Lawrie, C H; Enblad, G; Larsson, C; Berglund, M; Lui, W-O

2013-01-01

Deregulation of microRNA (miRNA) expression has been documented in diffuse large B-cell lymphoma (DLBCL). However, the impact of miRNAs and their machinery in DLBCL is not fully determined. Here, we assessed the role of miRNA expression and their processing genes in DLBCL development. Using microarray and RT-qPCR approaches, we quantified global miRNAs and core components of miRNA-processing genes expression in 75 DLBCLs (56 de novo and 19 transformed) and 10 lymph nodes (LN). Differential miRNA signatures were identified between DLBCLs and LNs, or between the de novo and transformed DLBCLs. We also identified subsets of miRNAs associated with germinal center B-cell phenotype, BCL6 and IRF4 expression, and clinical staging. In addition, we showed a significant over-expression of TARBP2 in de novo DLBCLs as compared with LNs, and decreased expression of DROSHA, DICER, TARBP2 and PACT in transformed as compared with de novo cases. Interestingly, cases with high TARBP2 and DROSHA expression had a poorer chemotherapy response. We further showed that TARBP2 can regulate miRNA-processing efficiency in DLBCLs, and its expression inhibition decreases cell growth and increases apoptosis in DLBCL cell lines. Our findings provide new insights for the understanding of miRNAs and its machinery in DLBCL

Condition monitoring of machinery using motor current signature analysis

International Nuclear Information System (INIS)

Kryter, R.C.; Haynes, H.D.

1989-01-01

Motor current signature analysis (MCSA) is a powerful monitoring tool for motor-driven equipment that provides a nonintrusive means for detecting the presence of mechanical and electrical abnormalities in the motor and the driven equipment, including altered conditions in the process ''downstream'' of the motor-driven equipment. It was developed at the Oak Ridge National Laboratory as a means for determining the effects of aging and service wear systems, but it is applicable to a broad range of machinery. MCSA is based on the recognition that an electric motor (ac or dc) driving a mechanical load acts as an efficient and permanently available transducer by sensing mechanical load variations, large and small, long-term and rapid, and converting them into variations in the induced current generated in the motor windings. These motor current variations are carried by the electrical cables processes as desired. Motor current signatures, obtained in both time and over time to provide early indication of degradation. Successful applications of MCSA technology (patent applied for) include not only motor-operated valves but also pumps of various designs, blowers, and air conditioning systems. Examples are presented briefly, and speculation regarding the applicability of MCSA to a broader range of equipment monitoring and production line testing is also given. 1 ref., 13 figs

The PurR regulon in Lactococcus lactis – transcriptional regulation of the purine nucleotide metabolism and translational machinery

DEFF Research Database (Denmark)

Jendresen, Christian Bille; Martinussen, Jan; Kilstrup, Mogens

2012-01-01

Purine nucleotides are either synthesized de novo from 5-phosphoribosyl-1-pyrophosphate (PRPP) or salvaged from the environment. In Lactococcus lactis, transcription of the de novo synthesis operons, purCSQLF and purDEK, has genetically been shown to be activated by the PurR protein when bound to......-related functions. Of special interest is the presence of PurBox motifs in rrn promoters, suggesting a novel connection between nucleotide availability and the translational machinery....

The pathological consequences of impaired genome integrity in humans; disorders of the DNA replication machinery.

Science.gov (United States)

O'Driscoll, Mark

2017-01-01

Accurate and efficient replication of the human genome occurs in the context of an array of constitutional barriers, including regional topological constraints imposed by chromatin architecture and processes such as transcription, catenation of the helical polymer and spontaneously generated DNA lesions, including base modifications and strand breaks. DNA replication is fundamentally important for tissue development and homeostasis; differentiation programmes are intimately linked with stem cell division. Unsurprisingly, impairments of the DNA replication machinery can have catastrophic consequences for genome stability and cell division. Functional impacts on DNA replication and genome stability have long been known to play roles in malignant transformation through a variety of complex mechanisms, and significant further insights have been gained from studying model organisms in this context. Congenital hypomorphic defects in components of the DNA replication machinery have been and continue to be identified in humans. These disorders present with a wide range of clinical features. Indeed, in some instances, different mutations in the same gene underlie different clinical presentations. Understanding the origin and molecular basis of these features opens a window onto the range of developmental impacts of suboptimal DNA replication and genome instability in humans. Here, I will briefly overview the basic steps involved in DNA replication and the key concepts that have emerged from this area of research, before switching emphasis to the pathological consequences of defects within the DNA replication network; the human disorders. Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

Real-time Monitoring System for Rotating Machinery with IoT-based Cloud Platform

Energy Technology Data Exchange (ETDEWEB)

Jeong, Haedong; Kim, Suhyun; Woo, Sunhee; Kim, Songhyun; Lee, Seungchul [Ulsan Nat’l Institute Science and Technology, Ulsan (Korea, Republic of)

2017-06-15

The objective of this research is to improve the efficiency of data collection from many machine components on smart factory floors using IoT(Internet of things) techniques and cloud platform, and to make it easy to update outdated diagnostic schemes through online deployment methods from cloud resources. The short-term analysis is implemented by a micro-controller, and it includes machine-learning algorithms for inferring snapshot information of the machine components. For long-term analysis, time-series and high-dimension data are used for root cause analysis by combining a cloud platform and multivariate analysis techniques. The diagnostic results are visualized in a webbased display dashboard for an unconstrained user access. The implementation is demonstrated to identify its performance in data acquisition and analysis for rotating machinery.

Real-time Monitoring System for Rotating Machinery with IoT-based Cloud Platform

International Nuclear Information System (INIS)

Jeong, Haedong; Kim, Suhyun; Woo, Sunhee; Kim, Songhyun; Lee, Seungchul

2017-01-01

The objective of this research is to improve the efficiency of data collection from many machine components on smart factory floors using IoT(Internet of things) techniques and cloud platform, and to make it easy to update outdated diagnostic schemes through online deployment methods from cloud resources. The short-term analysis is implemented by a micro-controller, and it includes machine-learning algorithms for inferring snapshot information of the machine components. For long-term analysis, time-series and high-dimension data are used for root cause analysis by combining a cloud platform and multivariate analysis techniques. The diagnostic results are visualized in a webbased display dashboard for an unconstrained user access. The implementation is demonstrated to identify its performance in data acquisition and analysis for rotating machinery.

Comparison of methods for separating vibration sources in rotating machinery

Science.gov (United States)

Klein, Renata

2017-12-01

Vibro-acoustic signatures are widely used for diagnostics of rotating machinery. Vibration based automatic diagnostics systems need to achieve a good separation between signals generated by different sources. The separation task may be challenging, since the effects of the different vibration sources often overlap. In particular, there is a need to separate between signals related to the natural frequencies of the structure and signals resulting from the rotating components (signal whitening), as well as a need to separate between signals generated by asynchronous components like bearings and signals generated by cyclo-stationary components like gears. Several methods were proposed to achieve the above separation tasks. The present study compares between some of these methods. The paper also presents a new method for whitening, Adaptive Clutter Separation, as well as a new efficient algorithm for dephase, which separates between asynchronous and cyclo-stationary signals. For whitening the study compares between liftering of the high quefrencies and adaptive clutter separation. For separating between the asynchronous and the cyclo-stationary signals the study compares between liftering in the quefrency domain and dephase. The methods are compared using both simulated signals and real data.

POLISH FARM MACHINERY MARKET AFTER ACCESSION TO THE EUROPEAN UNION – PRICES OF MEANS OF AGRICULTURAL MECHANIZATION

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Jan Pawlak

2014-12-01

Full Text Available VAT for farm machinery has been increased from 0 to 22% level since the accession to the EU. This was a main reason for rise in prices of a set of 34 farm machines which in June 2004 was by 28.1% higher than a year earlier. Thanks to the decrease in prices of the most expensive farm machines – harvester threshers, during the years 2006--2009 the price level was quite stable, even though prices of most machines rose. From 2010 moderate, but generally higher than the inflation rate rise in price of the set was observed. During the years 2003-2013 the price of the set of 34 farm machines grew more expensive by 66.7%, whilst the inflation index increased by 32.6%. In 2004, 2005, 2010 and 2013 the rate of increase of prices of farm machines was higher than dynamics of the inflation index (relatively by 16.8, 15.5, 3.5 and 3.1 per cent points. In spite of machinery price growth, the equivalent of the set of 34 machines in form of mass or volume of swine and cattle for slaughter, wheat, milk and potato was in 2013 by 1.0 to 33.3% lower than in 2003. The above mentioned set as related to the unit of mass of rye and sugar beet was by 4.1 and 40.3% more expensive.

DNA replication machinery is required for development in Drosophila.

Science.gov (United States)

Kohzaki, Hidetsugu; Asano, Maki; Murakami, Yota

2018-01-01

 In Drosophila , some factors involved in chromosome replication seem to be involved in gene amplification and endoreplication, which are actively utilized in particular tissue development, but direct evidence has not been shown. Therefore, we examined the effect of depletion of replication factors on these processes. First, we confirmed RNAi knockdown can be used for the depletion of replication factors by comparing the phenotypes of RNAi knockdown and deletion or point mutants of the components of DNA licensing factor, MCM2, MCM4 and Cdt1. Next, we found that tissue-specific RNAi knockdown of replication factors caused tissue-specific defects, probably due to defects in DNA replication. In particular, we found that depletion inhibited gene amplification of the chorion gene in follicle cells and endoreplication in salivary glands, showing that chromosomal DNA replication factors are required for these processes. Finally, using RNAi, we screened the genes for chromosomal DNA replication that affected tissue development. Interestingly, wing specific knockdown of Mcm10 induced wing formation defects. These results suggest that some components of chromosomal replication machinery are directly involved in tissue development.

Hijacking of the host SCF ubiquitin ligase machinery by plant pathogens

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Shimpei eMagori

2011-11-01

Full Text Available The SCF (SKP1-CUL1-F-box protein ubiquitin ligase complex mediates polyubiquitination of proteins targeted for degradation, thereby controlling a plethora of biological processes in eukaryotic cells. Although this ubiquitination machinery is found and functional only in eukaryotes, many non-eukaryotic pathogens also encode F-box proteins, the critical subunits of the SCF complex. Increasing evidence indicates that such non-eukaryotic F-box proteins play an essential role in subverting or exploiting the host ubiquitin/proteasome system for efficient pathogen infection. A recent bioinformatic analysis has identified more than 70 F-box proteins in 22 different bacterial species, suggesting that use of pathogen-encoded F-box effectors in the host cell may be a widespread infection strategy. In this review, we focus on plant pathogen-encoded F-box effectors, such as VirF of Agrobacterium tumefaciens, GALAs of Ralstonia solanacearum, and P0 of Poleroviruses, and discuss the molecular mechanism by which plant pathogens use these factors to manipulate the host cell for their own benefit.

Optimization of Hydraulic Machinery Bladings by Multilevel CFD Techniques

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Thum Susanne

2005-01-01

Full Text Available The numerical design optimization for complex hydraulic machinery bladings requires a high number of design parameters and the use of a precise CFD solver yielding high computational costs. To reduce the CPU time needed, a multilevel CFD method has been developed. First of all, the 3D blade geometry is parametrized by means of a geometric design tool to reduce the number of design parameters. To keep geometric accuracy, a special B-spline modification technique has been developed. On the first optimization level, a quasi-3D Euler code (EQ3D is applied. To guarantee a sufficiently accurate result, the code is calibrated by a Navier-Stokes recalculation of the initial design and can be recalibrated after a number of optimization steps by another Navier-Stokes computation. After having got a convergent solution, the optimization process is repeated on the second level using a full 3D Euler code yielding a more accurate flow prediction. Finally, a 3D Navier-Stokes code is applied on the third level to search for the optimum optimorum by means of a fine-tuning of the geometrical parameters. To show the potential of the developed optimization system, the runner blading of a water turbine having a specific speed n q = 41 1 / min was optimized applying the multilevel approach.

Model of Environmental Problems Priority Arising from the use of Environmental and Natural Resources in Machinery Sectors of Thailand

OpenAIRE

Sutthichaimethee Pruethsan; Sawangdee Yothin

2016-01-01

The objective of this research is to propose an indicator to evaluate environmental impacts from the Machinery sectors of Thailand, leading to more sustainable consumption and production in this sector of the economy. The factors used to calculate the Forward Linkage, Backward Linkage and Real Benefit were the Total Environmental Costs. The highest total environmental cost was Railway Equipment which needs to be resolved immediately because it uses natural resources more than its carrying cap...

Radiation survey of aircraft and heavy machinery scrap

International Nuclear Information System (INIS)

Idriss, Hajo; Salih, Isam; Gumaa, Elsadig; Yassin, Abbas; Yousif, E.H.; Abdel Hamid, Saad Eldeen M.; Sam, A.K.

2012-01-01

This study was conducted primarily to survey aircraft and heavy machinery at 30 locations within Khartoum State using handheld radiation survey meters to detect and identify any radiation sources that might be present and to estimate radiation dose levels. The survey has resulted in detection of 16 sealed sources of 90 Sr and one of 226 Ra in aircraft scrap. Of course, 90 Sr sources are used in military aircraft as temperature sensors while 226 Ra is used for indicating fuel levels. These sources were found intact without spreading radioactivity contamination; however, none was detected in heavy machine scrap. The levels of radiation dose measured at 0.1 m from the source fall within the range of 25.1–40.2 μSv/h with an average value of 33.52±4.06 μSv/h. These orphan sources have been separated from the scrap, tested for possible leakage, conditioned and stored in waste management facility. The result of this study has revealed without doubt that the scrap constitute a serious source of public exposure and highlights the importance of legislation making radiation monitoring of scrap in the country mandatory before it is sold to metal industry for reprocessing. - Highlights: ► Sealed radioactive sources ( 90 Sr and 226 Ra) were detected in aircraft scrap. ► No source was detected in heavy machine scrap. ► Radiation dose measured at 0.1 m from the source can be used to estimate exposure to public. ► Monitoring of scrap was found to be useful for protection (from orphan sources).

An Efficient Method of Vibration Diagnostics For Rotating Machinery Using a Decision Tree

Directory of Open Access Journals (Sweden)

Bo Suk Yang

2000-01-01

Full Text Available This paper describes an efficient method to automatize vibration diagnosis for rotating machinery using a decision tree, which is applicable to vibration diagnosis expert system. Decision tree is a widely known formalism for expressing classification knowledge and has been used successfully in many diverse areas such as character recognition, medical diagnosis, and expert systems, etc. In order to build a decision tree for vibration diagnosis, we have to define classes and attributes. A set of cases based on past experiences is also needed. This training set is inducted using a result-cause matrix newly developed in the present work instead of using a conventionally implemented cause-result matrix. This method was applied to diagnostics for various cases taken from published work. It is found that the present method predicts causes of the abnormal vibration for test cases with high reliability.

Comparative study of the usability of directional commands for two different conceptions of agricultural machinery

Directory of Open Access Journals (Sweden)

Ricardo Kozoroski Veiga

2015-09-01

Full Text Available Santa Catarina showed a significant increase in its fleet of agricultural machinery in the past 40 years. For the historical origins, the state experienced a strong farm household that grows in small portions of land. This small size of farms boosted the purchase of farm machinery such as small power tillers. It is estimated that in Santa Catarina there are more than 20,000 machines like these. The initial design of this machine its use is intended for operations on foot position for possessing it commands designed for the walking posture. But a large proportion of power tillers is used to transport cargo and personnel, by adapting implement that allows driving in the sitting position. This modification is incomplete because it does not allow the installation of steering wheel. Thus the inadequacy of command increases the difficulty in driving the vehicle and may potentiate human error, leading to the accident. To compare the usability and rate of errors in triggering commands power tiller and tractors, the work used two methods: analysis uses the model Usa-Design (U-D© and usability testing. A panel was positioned in front of the two agricultural machines simulated obstacles appearing on the right or left side of operator, in a position to rise or fall, and observed the reaction to the same stimulus. A sample of 53 volunteers showed that for all groups (experience levels the error was greater for the  power tiller. The tractor had 5.7 % error while the power tiller this value was 49.1 %. The results found by U-D© model was similar with the found in the test and pointed out serious deficiencies in functional and safety aspects of power tiller.

The microRNA machinery regulates fasting-induced changes in gene expression and longevity in Caenorhabditis elegans.

Science.gov (United States)

Kogure, Akiko; Uno, Masaharu; Ikeda, Takako; Nishida, Eisuke

2017-07-07

Intermittent fasting (IF) is a dietary restriction regimen that extends the lifespans of Caenorhabditis elegans and mammals by inducing changes in gene expression. However, how IF induces these changes and promotes longevity remains unclear. One proposed mechanism involves gene regulation by microRNAs (miRNAs), small non-coding RNAs (∼22 nucleotides) that repress gene expression and whose expression can be altered by fasting. To test this proposition, we examined the role of the miRNA machinery in fasting-induced transcriptional changes and longevity in C. elegans We revealed that fasting up-regulated the expression of the miRNA-induced silencing complex (miRISC) components, including Argonaute and GW182, and the miRNA-processing enzyme DRSH-1 (the ortholog of the Drosophila Drosha enzyme). Our lifespan measurements demonstrated that IF-induced longevity was suppressed by knock-out or knockdown of miRISC components and was completely inhibited by drsh-1 ablation. Remarkably, drsh-1 ablation inhibited the fasting-induced changes in the expression of the target genes of DAF-16, the insulin/IGF-1 signaling effector in C. elegans Fasting-induced transcriptome alterations were substantially and modestly suppressed in the drsh-1 null mutant and the null mutant of ain-1 , a gene encoding GW182, respectively. Moreover, miRNA array analyses revealed that the expression levels of numerous miRNAs changed after 2 days of fasting. These results indicate that components of the miRNA machinery, especially the miRNA-processing enzyme DRSH-1, play an important role in mediating IF-induced longevity via the regulation of fasting-induced changes in gene expression. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Vibration monitoring of EDF rotating machinery using artificial neural networks

International Nuclear Information System (INIS)

Alguindigue, I.E.; Loskiewicz-Buczak, A.; Uhrig, R.E.; Hamon, L.; Lefevre, F.

1991-01-01

Vibration monitoring of components in nuclear power plants has been used for a number of years. This technique involves the analysis of vibration data coming from vital components of the plant to detect features which reflect the operational state of machinery. The analysis leads to the identification of potential failures and their causes, and makes it possible to perform efficient preventive maintenance. Earlydetection is important because it can decrease the probability of catastrophic failures, reduce forced outgage, maximize utilization of available assets, increase the life of the plant, and reduce maintenance costs. This paper documents our work on the design of a vibration monitoring methodology based on neural network technology. This technology provides an attractive complement to traditional vibration analysis because of the potential of neural networks to operate in real-time mode and to handle data which may be distorted or noisy. Our efforts have been concentrated on the analysis and classification of vibration signatures collected by Electricite de France (EDF). Two neural networks algorithms were used in our project: the Recirculation algorithm and the Backpropagation algorithm. Although this project is in the early stages of development it indicates that neural networks may provide a viable methodology for monitoring and diagnostics of vibrating components. Our results are very encouraging

Human Parvovirus B19 Utilizes Cellular DNA Replication Machinery for Viral DNA Replication.

Science.gov (United States)

Zou, Wei; Wang, Zekun; Xiong, Min; Chen, Aaron Yun; Xu, Peng; Ganaie, Safder S; Badawi, Yomna; Kleiboeker, Steve; Nishimune, Hiroshi; Ye, Shui Qing; Qiu, Jianming

2018-03-01

Human parvovirus B19 (B19V) infection of human erythroid progenitor cells (EPCs) induces a DNA damage response and cell cycle arrest at late S phase, which facilitates viral DNA replication. However, it is not clear exactly which cellular factors are employed by this single-stranded DNA virus. Here, we used microarrays to systematically analyze the dynamic transcriptome of EPCs infected with B19V. We found that DNA metabolism, DNA replication, DNA repair, DNA damage response, cell cycle, and cell cycle arrest pathways were significantly regulated after B19V infection. Confocal microscopy analyses revealed that most cellular DNA replication proteins were recruited to the centers of viral DNA replication, but not the DNA repair DNA polymerases. Our results suggest that DNA replication polymerase δ and polymerase α are responsible for B19V DNA replication by knocking down its expression in EPCs. We further showed that although RPA32 is essential for B19V DNA replication and the phosphorylated forms of RPA32 colocalized with the replicating viral genomes, RPA32 phosphorylation was not necessary for B19V DNA replication. Thus, this report provides evidence that B19V uses the cellular DNA replication machinery for viral DNA replication. IMPORTANCE Human parvovirus B19 (B19V) infection can cause transient aplastic crisis, persistent viremia, and pure red cell aplasia. In fetuses, B19V infection can result in nonimmune hydrops fetalis and fetal death. These clinical manifestations of B19V infection are a direct outcome of the death of human erythroid progenitors that host B19V replication. B19V infection induces a DNA damage response that is important for cell cycle arrest at late S phase. Here, we analyzed dynamic changes in cellular gene expression and found that DNA metabolic processes are tightly regulated during B19V infection. Although genes involved in cellular DNA replication were downregulated overall, the cellular DNA replication machinery was tightly

Optimization of Ventilation and Alarm Setting During the Process of Ammonia Leak in Refrigeration Machinery Room Based on Numerical Simulation

Directory of Open Access Journals (Sweden)

Dongliang Liu

2017-03-01

Full Text Available In order to optimize the ventilation effect of ammonia leakage in the refrigeration machinery room, a food processing enterprise is selected as the subject investigated. The velocity and concentration field distribution during the process of ammonia leakage are discussed through simulation of refrigeration machinery room using CFD software. The ventilation system of the room is optimized in three aspects which are named air distribution, ventilation volume and discharge outlet. The influence of the ammonia alarm system through ventilation is also analyzed. The results show that it will be better to set the discharge outlet at the top of the plant than at the side of the wall, and the smaller of the distance between the air outlet and the ammonia gathering area, the better of the effect of ventilation will be. The air flow can be improved and the vortex flow can be reduced if the ventilation volume, the number of air vents and the exhaust velocity are reasonably arranged. Not only the function of the alarm could be ensured, but also the scope of the detection area could be enlarged if the detectors are set on the ceiling of the refrigeration units or the ammonia storage vessel.

The cooperation between the autophagy machinery and the inflammasome to implement an appropriate innate immune response: do they regulate each other?

OpenAIRE

Abdelaziz, Dalia H. A.; Khalil, Hany; Cormet-Boyaka, Estelle; Amer, Amal O.

2015-01-01

Autophagy is originally described as the main catabolic pathway responsible for maintaining intracellular nutritional homeosta-sis that involves the formation of a unique vacuole, the autophago-some, and the interaction with the endosome-lysosome pathways. This conserved machinery plays a key role in immune-protection against different invaders, including pathogenic bacteria, intracellular parasites, and some viruses like herpes simplex and hepatitis C virus. Importantly, autophagy is linked ...

The novel chloroplast outer membrane kinase KOC1 is a required component of the plastid protein import machinery.

Science.gov (United States)

Zufferey, Mónica; Montandon, Cyrille; Douet, Véronique; Demarsy, Emilie; Agne, Birgit; Baginsky, Sacha; Kessler, Felix

2017-04-28

The biogenesis and maintenance of cell organelles such as mitochondria and chloroplasts require the import of many proteins from the cytosol, a process that is controlled by phosphorylation. In the case of chloroplasts, the import of hundreds of different proteins depends on translocons at the outer and inner chloroplast membrane (TOC and TIC, respectively) complexes. The essential protein TOC159 functions thereby as an import receptor. It has an N-terminal acidic (A-) domain that extends into the cytosol, controls receptor specificity, and is highly phosphorylated in vivo However, kinases that phosphorylate the TOC159 A-domain to enable protein import have remained elusive. Here, using co-purification with TOC159 from Arabidopsis , we discovered a novel component of the chloroplast import machinery, the regulatory kinase at the outer chloroplast membrane 1 (KOC1). We found that KOC1 is an integral membrane protein facing the cytosol and stably associates with TOC. Moreover, KOC1 phosphorylated the A-domain of TOC159 in vitro , and in mutant koc1 chloroplasts, preprotein import efficiency was diminished. koc1 Arabidopsis seedlings had reduced survival rates after transfer from the dark to the light in which protein import into plastids is required to rapidly complete chloroplast biogenesis. In summary, our data indicate that KOC1 is a functional component of the TOC machinery that phosphorylates import receptors, supports preprotein import, and contributes to efficient chloroplast biogenesis. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Increasing Nucleosome Occupancy Is Correlated with an Increasing Mutation Rate so Long as DNA Repair Machinery Is Intact

Science.gov (United States)

Taylor, Jared F.; Khattab, Omar S.; Chen, Yu-Han; Chen, Yumay; Jacobsen, Steven E.; Wang, Ping H.

2015-01-01

Deciphering the multitude of epigenomic and genomic factors that influence the mutation rate is an area of great interest in modern biology. Recently, chromatin has been shown to play a part in this process. To elucidate this relationship further, we integrated our own ultra-deep sequenced human nucleosomal DNA data set with a host of published human genomic and cancer genomic data sets. Our results revealed, that differences in nucleosome occupancy are associated with changes in base-specific mutation rates. Increasing nucleosome occupancy is associated with an increasing transition to transversion ratio and an increased germline mutation rate within the human genome. Additionally, cancer single nucleotide variants and microindels are enriched within nucleosomes and both the coding and non-coding cancer mutation rate increases with increasing nucleosome occupancy. There is an enrichment of cancer indels at the theoretical start (74 bp) and end (115 bp) of linker DNA between two nucleosomes. We then hypothesized that increasing nucleosome occupancy decreases access to DNA by DNA repair machinery and could account for the increasing mutation rate. Such a relationship should not exist in DNA repair knockouts, and we thus repeated our analysis in DNA repair machinery knockouts to test our hypothesis. Indeed, our results revealed no correlation between increasing nucleosome occupancy and increasing mutation rate in DNA repair knockouts. Our findings emphasize the linkage of the genome and epigenome through the nucleosome whose properties can affect genome evolution and genetic aberrations such as cancer. PMID:26308346

Increasing Nucleosome Occupancy Is Correlated with an Increasing Mutation Rate so Long as DNA Repair Machinery Is Intact.

Directory of Open Access Journals (Sweden)

Puya G Yazdi

Full Text Available Deciphering the multitude of epigenomic and genomic factors that influence the mutation rate is an area of great interest in modern biology. Recently, chromatin has been shown to play a part in this process. To elucidate this relationship further, we integrated our own ultra-deep sequenced human nucleosomal DNA data set with a host of published human genomic and cancer genomic data sets. Our results revealed, that differences in nucleosome occupancy are associated with changes in base-specific mutation rates. Increasing nucleosome occupancy is associated with an increasing transition to transversion ratio and an increased germline mutation rate within the human genome. Additionally, cancer single nucleotide variants and microindels are enriched within nucleosomes and both the coding and non-coding cancer mutation rate increases with increasing nucleosome occupancy. There is an enrichment of cancer indels at the theoretical start (74 bp and end (115 bp of linker DNA between two nucleosomes. We then hypothesized that increasing nucleosome occupancy decreases access to DNA by DNA repair machinery and could account for the increasing mutation rate. Such a relationship should not exist in DNA repair knockouts, and we thus repeated our analysis in DNA repair machinery knockouts to test our hypothesis. Indeed, our results revealed no correlation between increasing nucleosome occupancy and increasing mutation rate in DNA repair knockouts. Our findings emphasize the linkage of the genome and epigenome through the nucleosome whose properties can affect genome evolution and genetic aberrations such as cancer.

The Machinery of Authoritarian Care: Dramatising Breast Cancer Treatment in 1970s Britain.

Science.gov (United States)

Toon, Elizabeth

2014-08-01

This article examines the professional and public response to the television play Through the Night , which aired on BBC1 in December 1975. One of the first British mass media portrayals of a woman's experience being treated for breast cancer, this play attracted a large audience and considerable attention from both critics and everyday viewers. My analysis of the play draws on sources documenting expert responses to the play in its production stages, as well as critics' and viewers' responses to what the play said about breast cancer treatment in particular, and about Britons' experiences of medical institutions more broadly. Together, I argue, these sources help us see how Through the Night 's critique of what one expert called 'the machinery of authoritarian care' reverberated with and supported the efforts of professionals anxious to improve patient experience, and how it crystallised the concerns of activists and everyday viewers.

Nuclear export of human hepatitis B virus core protein and pregenomic RNA depends on the cellular NXF1-p15 machinery.

Science.gov (United States)

Yang, Ching-Chun; Huang, Er-Yi; Li, Hung-Cheng; Su, Pei-Yi; Shih, Chiaho

2014-01-01

Hepatitis B virus (HBV) core protein (HBc) can shuttle between nucleus and cytoplasm. Cytoplasm-predominant HBc is clinically associated with severe liver inflammation. Previously, we found that HBc arginine-rich domain (ARD) can associate with a host factor NXF1 (TAP) by coimmunoprecipitation. It is well known that NXF1-p15 heterodimer can serve as a major export receptor of nuclear mRNA as a ribonucleoprotein complex (RNP). In the NXF1-p15 pathway, TREX (transcription/export) complex plays an important role in coupling nuclear pre-mRNA processing with mRNA export in mammalian cells. Here, we tested the hypothesis whether HBc and HBV specific RNA can be exported via the TREX and NXF1-p15 mediated pathway. We demonstrated here that HBc can physically and specifically associate with TREX components, and the NXF1-p15 export receptor by coimmunoprecipitation. Accumulation of HBc protein in the nucleus can be induced by the interference with TREX and NXF1-p15 mediated RNA export machinery. HBV transcripts encodes a non-spliced 3.5 kb pregenomic RNA (pgRNA) which can serve as a template for reverse transcription. Cytoplasmic HBV pgRNA appeared to be reduced by siRNA treatment specific for the NXF1-p15 complex by quantitative RT-qPCR and Northern blot analyses. This result suggests that the pgRNA was also exported via the NXF1-p15 machinery. We entertain the hypothesis that HBc protein can be exported as an RNP cargo via the mRNA export pathway by hijacking the TREX and NXF1-p15 complex. In our current and previous studies, HBc is not required for pgRNA accumulation in the cytoplasm. Furthermore, HBc ARD can mediate nuclear export of a chimeric protein containing HBc ARD in a pgRNA-independent manner. Taken together, it suggests that while both pgRNA and HBc protein exports are dependent on NXF1-p15, they are using the same export machinery in a manner independent of each other.

Quantum-mechanical machinery for rational decision-making in classical guessing game

Science.gov (United States)

Bang, Jeongho; Ryu, Junghee; Pawłowski, Marcin; Ham, Byoung S.; Lee, Jinhyoung

2016-02-01

In quantum game theory, one of the most intriguing and important questions is, “Is it possible to get quantum advantages without any modification of the classical game?” The answer to this question so far has largely been negative. So far, it has usually been thought that a change of the classical game setting appears to be unavoidable for getting the quantum advantages. However, we give an affirmative answer here, focusing on the decision-making process (we call ‘reasoning’) to generate the best strategy, which may occur internally, e.g., in the player’s brain. To show this, we consider a classical guessing game. We then define a one-player reasoning problem in the context of the decision-making theory, where the machinery processes are designed to simulate classical and quantum reasoning. In such settings, we present a scenario where a rational player is able to make better use of his/her weak preferences due to quantum reasoning, without any altering or resetting of the classically defined game. We also argue in further analysis that the quantum reasoning may make the player fail, and even make the situation worse, due to any inappropriate preferences.

Quantum-mechanical machinery for rational decision-making in classical guessing game.

Science.gov (United States)

Bang, Jeongho; Ryu, Junghee; Pawłowski, Marcin; Ham, Byoung S; Lee, Jinhyoung

2016-02-15

In quantum game theory, one of the most intriguing and important questions is, "Is it possible to get quantum advantages without any modification of the classical game?" The answer to this question so far has largely been negative. So far, it has usually been thought that a change of the classical game setting appears to be unavoidable for getting the quantum advantages. However, we give an affirmative answer here, focusing on the decision-making process (we call 'reasoning') to generate the best strategy, which may occur internally, e.g., in the player's brain. To show this, we consider a classical guessing game. We then define a one-player reasoning problem in the context of the decision-making theory, where the machinery processes are designed to simulate classical and quantum reasoning. In such settings, we present a scenario where a rational player is able to make better use of his/her weak preferences due to quantum reasoning, without any altering or resetting of the classically defined game. We also argue in further analysis that the quantum reasoning may make the player fail, and even make the situation worse, due to any inappropriate preferences.

3rd International Conference on Condition Monitoring of Machinery in Non-Stationary Operations

CERN Document Server

Rubini, Riccardo; D'Elia, Gianluca; Cocconcelli, Marco; Chaari, Fakher; Zimroz, Radoslaw; Bartelmus, Walter; Haddar, Mohamed

2014-01-01

This book presents the processings of the third edition of the Condition Monitoring of Machinery in Non-Stationary Operations (CMMNO13) which was held in Ferrara, Italy. This yearly event merges an international community of researchers who met – in 2011 in Wroclaw (Poland) and in 2012 in Hammamet (Tunisia) – to discuss issues of diagnostics of rotating machines operating in complex motion and/or load conditions. The growing interest of the industrial world on the topics covered by the CMMNO13 involves the fields of packaging, automotive, agricultural, mining, processing and wind machines in addition to that of the systems for data acquisition.The participation of speakers and visitors from industry makes the event an opportunity for immediate assessment of the potential applications of advanced methodologies for the signal analysis. Signals acquired from machines often contain contributions from several different components as well as noise. Therefore, the major challenge of condition monitoring is to po...

Autophagy response: manipulating the mTOR-controlled machinery by amino acids and pathogens.

Science.gov (United States)

Fader, Claudio Marcelo; Aguilera, Milton Osmar; Colombo, María Isabel

2015-10-01

Macroautophagy is a self-degradative process that normally maintains cellular homeostasis via a lysosomal pathway. It is induced by different stress signals, including nutrients and growth factors' restriction as well as pathogen invasions. These stimuli are modulated by the serine/threonine protein kinase mammalian target of rapamycin (mTOR) which control not only autophagy but also protein translation and gene expression. This review focuses on the important role of mTOR as a master regulator of cell growth and the autophagy pathway. Here, we have discussed the role of intracellular amino acid availability and intracellular pH in the redistribution of autophagic structures, which may contribute to mammalian target of rapamycin complex 1 (mTORC1) activity regulation. We have also discussed that mTORC1 complex and components of the autophagy machinery are localized at the lysosomal surface, representing a fascinating mechanism to control the metabolism, cellular clearance and also to restrain invading intracellular pathogens.

Cavitation and gas-liquid flow in fluid machinery and devices. FED-Volume 190

International Nuclear Information System (INIS)

O'Hern, T.J.; Kim, J.H.; Morgan, W.B.; Furuya, O.

1994-01-01

Cavitation and gas-liquid two-phase flow have remained important areas in many industrial applications and constantly provided challenges for academic researchers and industrial practitioners alike. Cavitation and two-phase flow commonly occur in fluid machinery such as pumps, propellers, and fluid devices such as orifices, valves, and diffusers. Cavitation not only degrades the performance of these machines and devices but deteriorates the materials. Gas-liquid two-phase flow has also been known to degrade the performance of pumps and propellers and can often induce an instability. The industrial applications of cavitation and two-phase flow can be found in power plants, ship propellers, hydrofoils, and aerospace equipment, to name but a few. The papers presented in this volume reflect the variety and richness of cavitation and gas-liquid two-phase flow in various flow transporting components and the increasing role they play in modern and conventional technologies. Separate abstracts were prepared for 35 papers in this book

Neo-industrialization of Kuzbass economy in innovative development of coal industry and machinery

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Balabanova Anna

2017-01-01

Full Text Available As part of rental-resource model and access restrictions to capital and new technologies, the attempts to encourage import substitution made by the government do not generate innovative type of economic development and reconstruction of economy’s manufacturing industry competitive on the domestic market. In conditions of searching for a new model of Russian economy development the analysis of a number of problems such as industry degradation, reducing the competitiveness of Russian producers, the growth of structural imbalances and overcoming the negative macroeconomic trends becomes strategically important. This raises the value of neo-industrialization as the integrator of resource security, scientific and industrial potential of Russian economy and high capacity of the domestic market in the innovative development system. A special role neo-industrialization plays for the economic development of old industrial regions, which include Kuzbass (Western Siberia, Russia. Neo-industrial development of Kuzbass economy should be initiated by the technological modernization of mining machinery and innovative development of coal industry.

[Investigation about prevention behavior for dust workers in machinery, ceramic, and metallurgy industry].

Science.gov (United States)

Shen, Fu-hai; Ma, Qing-kun; Xiao, Shu-yu; Cui, Feng-tao; Meng, Qing-di; Yang, Xiu-qing; Qi, Hui-sheng; Fan, Xue-yun; Yao, San-qiao

2011-01-01

The purposes of this thesis were to study the behavior about workers exposed to dust and provide scientific basis for health promotion. We designed a questionnaire and carry it on the 746 dust workers in the 3 representative corporations of Machinery, Ceramic, and Metallurgy Industry. All data were input into computer. And a database was established with Excel. SPSS11.5 statistical analysis software was used to analyze the influence on protecting behavioral between the application of qualifications, different jobs, training or protection, and other aspects etc. The rates were 94.4% and 75.3% about the regular physical examination and requirements for protective equipment. The rate of choosing an effective way of protection was generally low (15.4%). There was significant difference for among different educational background workers (P Metallurgy Industry. Those who were not educated had a lower using rate about the protection behavior, regular physical examination, and requirements for protective equipment than those educated.

Liquid metal current collectors for high-speed rotating machinery

International Nuclear Information System (INIS)

Carr, S.L.

1976-01-01

Recent interest in superconducting motors and generators has created a renewed interest in homopolar machinery. Homopolar machine designs have always been limited by the need for compact, high-current, low-voltage, sliding electrical curent collectors. Conventional graphite-based solid brushes are inadequate for use in homopolar machines. Liquid metals, under certain conditions of relative sliding velocities, electrical currents, and magnetic fields are known to be capable of performing well in homopolar machines. An effort to explore the capabilities and limits of a tongue-and-groove style current collector, utilizing sodium-potassium eutectic alloy (NaK) as the working fluid in high sliding speed operation is reported here. A double current collector generator model with a 14.5-cm maximum rotor diameter, 20,000 rpm rotational capability, and electrical current carrying ability was constructed and operated successfully at a peripheral velocity of 125 m/s. The limiting factor in these experiments was a high-speed fluid-flow instability resulting in the ejection of the working fluid from the operating portions of the collectors. The effects of collector size and geometry, working fluid (NaK or water), and cover gas pressure are reported. Hydrodynamic frictional torque-speed curves are given for the two fluids and for several geometries. Electrical resistances as a function of peripheral velocity at 60 amperes are reported, and the phenomenology of the high-speed fluid-flow instabilities is discussed. The possibility of long-term high-speed operation of current collectors of the tongue-and-groove type, along with experimental and theoretical hydrodynamic friction losses at high peripheral velocities, is considered

Application of an automatic pattern recognition for aleatory signals for the surveillance of nuclear reactor and rotating machinery

International Nuclear Information System (INIS)

Nascimento, J.A. do.

1982-02-01

An automatic pattern recognition program PSDREC, developed for the surveillance of nuclear reactor and rotating machinery is described and the relevant theory is outlined. Pattern recognition analysis of noise signals is a powerful technique for assessing 'system normality' in dynamic systems. This program, with applies 8 statistical tests to calculated power spectral density (PSD) distribution, was earlier installed in a PDP-11/45 computer at IPEN. To analyse recorded signals from three systems, namely an operational BWR power reactor (neutron signals), a water pump and a diesel engine (vibration signals) this technique was used. Results of the tests are considered satisfactory. (Author) [pt

Design and modeling of an advanced marine machinery system including waste heat recovery and removal of sulphur oxides

DEFF Research Database (Denmark)

Frimann Nielsen, Rasmus; Haglind, Fredrik; Larsen, Ulrik

2013-01-01

-stroke diesel engine and a conventional waste heat recovery system. The results suggest that an organic Rankine cycle placed after the conventional waste heat recovery system is able to extract the sulphuric acid from the exhaust gas, while at the same time increase power generation from waste heat by 32...... consists of a two-stroke diesel engine, the wet sulphuric process for sulphur removal and an advanced waste heat recovery system including a conventional steam Rankine cycle and an organic Rankine cycle. The results are compared with those of a state-of-the-art machinery system featuring a two...

From DNA to proteins via the ribosome: Structural insights into the workings of the translation machinery

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Agirrezabala Xabier

2010-04-01

Full Text Available Abstract Understanding protein synthesis in bacteria and humans is important for understanding the origin of many human diseases and devising treatments for them. Over the past decade, the field of structural biology has made significant advances in the visualisation of the molecular machinery involved in protein synthesis. It is now possible to discern, at least in outline, the way that interlocking ribosomal components and factors adapt their conformations throughout this process. The determination of structures in various functional contexts, along with the application of kinetic and fluorescent resonance energy transfer approaches to the problem, has given researchers the frame of reference for what remains as the greatest challenge: the complete dynamic portrait of protein synthesis in the cell.

Modulation of DNA methylation machineries in Japanese rice fish (Oryzias latipes) embryogenesis by ethanol and 5-azacytidine.

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Dasmahapatra, Asok K; Khan, Ikhlas A

2016-01-01

As a sequel of our investigations on the impact of epigenome in inducing fetal alcohol spectrum disorder (FASD) phenotypes in Japanese rice fish, we have investigated on several DNA methylation machinery genes including DNA methyl transferase 3ba (dnmt3ba) and methyl binding proteins (MBPs), namely, mbd1b, mbd3a, mbd3b, and mecp2 at the transcription level. Studies were made during normal development, from 0day post fertilization (dpf) to hatching, and also exposing the fertilized eggs to ethanol or a DNMT inhibitor, 5-azacytidine (5-azaC). We observed that during development, all these genes followed distinct expression patterns, generally high mRNA copies in early phases (0-1dpf) and significantly low mRNA copies prior to or after hatching. Ethanol (100-500mM, 0-2dpf) was unable to alter any of these mRNAs in 2dpf; additional four day (2-6dpf) maintenance of these embryos in ethanol-free environment, on 6dpf, was also unable to establish any significant difference in these mRNA levels in comparison with the corresponding controls. However, continuous exposure of fertilized eggs in 300mM ethanol, 0-6dpf, showed significantly high mRNA copies only in MBPs (mbd1b, mbd3a, mbd3b, mecp2). 5-azaC (2mM) on 2dpf was able to enhance only mbd3b mRNA. Removal of 5-azaC and maintenance of these embryos in clean medium, 2-6dpf, showed significantly enhanced mbd3b and mecp2 mRNAs compared to corresponding controls on 6dpf. Our studies showed that in Japanese rice fish embryogenesis both ethanol and 5-azaC have the potential to specifically modulate the developmental rhythm of DNA methylation machineries. Published by Elsevier Inc.

Performance Analysis of Wind-Induced Piezoelectric Vibration Bimorph Cantilever for Rotating Machinery

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Gongbo Zhou

2015-01-01

Full Text Available Harvesting the energy contained in the running environment of rotating machinery would be a good way to supplement energy to the wireless sensor. In this paper, we take piezoelectric bimorph cantilever beam with parallel connection mode as energy collector and analyze the factors which can influence the generation performance. First, a modal response theory model is built. Second, the static analysis, modal analysis, and piezoelectric harmonic response analysis of the wind-induced piezoelectric bimorph cantilever beam are given in detail. Finally, an experiment is also conducted. The results show that wind-induced piezoelectric bimorph cantilever beam has low resonant frequency and stable output under the first modal mode and can achieve the maximum output voltage under the resonant condition. The output voltage increases with the increase of the length and width of wind-induced piezoelectric bimorph cantilever beam, but the latter increasing amplitude is relatively smaller. In addition, the output voltage decreases with the increase of the thickness and the ratio of metal substrate to piezoelectric patches thickness. The experiment showed that the voltage amplitude generated by the piezoelectric bimorph cantilever beam can reach the value simulated in ANSYS, which is suitable for actual working conditions.

A Decision-Analytic Feasibility Study of Upgrading Machinery at a Tools Workshop

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M. L. Chew Hernandez

2012-04-01

Full Text Available This paper presents the evaluation, from a Decision Analysis point of view, of the feasibility of upgrading machinery at an existing metal-forming workshop. The Integral Decision Analysis (IDA methodology is applied to clarify the decision and develop a decision model. One of the key advantages of the IDA is its careful selection of the problem frame, allowing a correct problem definition. While following most of the original IDA methodology, an addition to this methodology is proposed in this work, that of using the strategic Means-Ends Objective Network as a backbone for the development of the decision model. The constructed decision model uses influence diagrams to include factual operator and vendor expertise, simulation to evaluate the alternatives and a utility function to take into account the risk attitude of the decision maker. Three alternatives are considered: Base (no modification, CNC (installing an automatic lathe and CF (installation of an automatic milling machine. The results are presented as a graph showing zones in which a particular alternative should be selected. The results show the potential of IDA to tackle technical decisions that are otherwise approached without the due care.

Effect of ursodeoxycholic acid on bile acid profiles and intestinal detoxification machinery in primary biliary cirrhosis and health.

Science.gov (United States)

Dilger, Karin; Hohenester, Simon; Winkler-Budenhofer, Ursula; Bastiaansen, Barbara A J; Schaap, Frank G; Rust, Christian; Beuers, Ulrich

2012-07-01

Ursodeoxycholic acid (UDCA) exerts anticholestatic, antifibrotic and antiproliferative effects in primary biliary cirrhosis (PBC) via mechanisms not yet fully understood. Its adequate biliary enrichment is considered mandatory for therapeutic efficacy. However, precise determination of biliary enrichment of UDCA is not possible in clinical practice. Therefore, we investigated (i) the relationship between biliary enrichment and plasma pharmacokinetics of UDCA, (ii) the effect of UDCA on plasma and biliary bile acid composition and conjugation patterns, and (iii) on the intestinal detoxification machinery in patients with PBC and healthy controls. In 11 PBC patients and 11 matched healthy subjects, cystic bile and duodenal tissue were collected before and after 3 weeks of administration of UDCA (15 mg/kg/day). Extensive pharmacokinetic profiling of bile acids was performed. The effect of UDCA on the intestinal detoxification machinery was studied by quantitative PCR and Western blotting. The relative fraction of UDCA and its conjugates in plasma at trough level[x] correlated with their biliary enrichment[y] (r=0.73, p=0.0001, y=3.65+0.49x). Taurine conjugates of the major hydrophobic bile acid, chenodeoxycholic acid, were more prominent in bile of PBC patients than in that of healthy controls. Biliary bile acid conjugation patterns normalized after treatment with UDCA. UDCA induced duodenal expression of key export pumps, BCRP and P-glycoprotein. Biliary and trough plasma enrichment of UDCA are closely correlated in PBC and health. Taurine conjugation may represent an adaptive mechanism in PBC against chenodeoxycholic acid-mediated bile duct damage. UDCA may stabilize small intestinal detoxification by upregulation of efflux pumps. Copyright © 2012. Published by Elsevier B.V.

Functional simulations of power electronics components in series-hybrid machinery for the needs of OEM

Energy Technology Data Exchange (ETDEWEB)

Liukkonen, M.; Hentunen, A.; Kyyrae, J. (Department of Electrical Engineering, Helsinki University of Technology, Espoo (Finland)); Suomela, J. (Department of Automation and Systems, Helsinki University of Technology, Espoo (Finland))

2008-07-01

A method for rapid control prototyping of the series-hybrid transmission system is proposed in this paper. The rapid control prototyping needs simulation submodels from all system components in order to develop supervisory control software. The same simulation models can also be used to optimize the drive train. The target framework for the rapid control prototyping method is the original equipment manufacturer (OEM), where the objective is to build devices from subcontractor's components. The machinery industry, as a target group, uses high power ratings for the creation of motion, which leads to high voltage and current values used in the system. Therefore, prototyping is started with careful simulations. This paper also seeks to create a general idea about the structure of the series-hybrid power transmission and assists the start of the process for designing the supervisory control. (orig.)

Caenorhabditis elegans histone methyltransferase MET-2 shields the male X chromosome from checkpoint machinery and mediates meiotic sex chromosome inactivation.

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Paula M Checchi

2011-09-01

Full Text Available Meiosis is a specialized form of cellular division that results in the precise halving of the genome to produce gametes for sexual reproduction. Checkpoints function during meiosis to detect errors and subsequently to activate a signaling cascade that prevents the formation of aneuploid gametes. Indeed, asynapsis of a homologous chromosome pair elicits a checkpoint response that can in turn trigger germline apoptosis. In a heterogametic germ line, however, sex chromosomes proceed through meiosis with unsynapsed regions and are not recognized by checkpoint machinery. We conducted a directed RNAi screen in Caenorhabditis elegans to identify regulatory factors that prevent recognition of heteromorphic sex chromosomes as unpaired and uncovered a role for the SET domain histone H3 lysine 9 histone methyltransferase (HMTase MET-2 and two additional HMTases in shielding the male X from checkpoint machinery. We found that MET-2 also mediates the transcriptional silencing program of meiotic sex chromosome inactivation (MSCI but not meiotic silencing of unsynapsed chromatin (MSUC, suggesting that these processes are distinct. Further, MSCI and checkpoint shielding can be uncoupled, as double-strand breaks targeted to an unpaired, transcriptionally silenced extra-chromosomal array induce checkpoint activation in germ lines depleted for met-2. In summary, our data uncover a mechanism by which repressive chromatin architecture enables checkpoint proteins to distinguish between the partnerless male X chromosome and asynapsed chromosomes thereby shielding the lone X from inappropriate activation of an apoptotic program.

Racial Differences in Expression Levels of miRNA Machinery-Related Genes, Dicer, Drosha, DGCR8, and AGO2, in Asian Korean Papillary Thyroid Carcinoma and Comparative Validation Using the Cancer Genome Atlas

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Jaegil Kim

2017-01-01

Full Text Available Aberrant regulation of microRNA (miRNA machinery components is associated with various human cancers, including papillary thyroid carcinoma (PTC, which is the most common type of thyroid cancer, and a higher prevalent female malignancy. The purpose of this study is to investigate racial differences in mRNA expression levels of four miRNA machinery components, Dicer, Drosha, DGCR8, and AGO2, and their correlations with clinicopathological characteristics. Forty PTC samples from female Asian Korean PTC patients were enrolled. Using qPCR, we examined mRNA expression levels of the components and next validated our results by comparison with results of female white American in the TCGA PTC project. Interestingly, mRNA expression levels of the selected factors were altered in the TCGA PTC samples. However, only Drosha showed a significantly lower expression level in Asian Korean PTC samples. Furthermore, the mRNA expression levels of the four components showed no association with clinicopathological characteristics in both groups. On the other hand, positive correlations were observed between altered mRNA expression levels of Dicer and Drosha and DGCR8 and Drosha in TCGA PTC samples. These findings collectively revealed that altered mRNA expression levels of miRNA machinery components might be responsible for racial differences in the carcinogenesis of PTC.

EEMD Independent Extraction for Mixing Features of Rotating Machinery Reconstructed in Phase Space

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Zaichao Ma

2015-04-01

Full Text Available Empirical Mode Decomposition (EMD, due to its adaptive decomposition property for the non-linear and non-stationary signals, has been widely used in vibration analyses for rotating machinery. However, EMD suffers from mode mixing, which is difficult to extract features independently. Although the improved EMD, well known as the ensemble EMD (EEMD, has been proposed, mode mixing is alleviated only to a certain degree. Moreover, EEMD needs to determine the amplitude of added noise. In this paper, we propose Phase Space Ensemble Empirical Mode Decomposition (PSEEMD integrating Phase Space Reconstruction (PSR and Manifold Learning (ML for modifying EEMD. We also provide the principle and detailed procedure of PSEEMD, and the analyses on a simulation signal and an actual vibration signal derived from a rubbing rotor are performed. The results show that PSEEMD is more efficient and convenient than EEMD in extracting the mixing features from the investigated signal and in optimizing the amplitude of the necessary added noise. Additionally PSEEMD can extract the weak features interfered with a certain amount of noise.

The Intracellular Localization of the Vanillin Biosynthetic Machinery in Pods of Vanilla planifolia.

Science.gov (United States)

Gallage, Nethaji J; Jørgensen, Kirsten; Janfelt, Christian; Nielsen, Agnieszka J Z; Naake, Thomas; Dunski, Eryk; Dalsten, Lene; Grisoni, Michel; Møller, Birger Lindberg

2018-02-01

Vanillin is the most important flavor compound in the vanilla pod. Vanilla planifolia vanillin synthase (VpVAN) catalyzes the conversion of ferulic acid and ferulic acid glucoside into vanillin and vanillin glucoside, respectively. Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) of vanilla pod sections demonstrates that vanillin glucoside is preferentially localized within the mesocarp and placental laminae whereas vanillin is preferentially localized within the mesocarp. VpVAN is present as the mature form (25 kDa) but, depending on the tissue and isolation procedure, small amounts of the immature unprocessed form (40 kDa) and putative oligomers (50, 75 and 100 kDa) may be observed by immunoblotting using an antibody specific to the C-terminal sequence of VpVAN. The VpVAN protein is localized within chloroplasts and re-differentiated chloroplasts termed phenyloplasts, as monitored during the process of pod development. Isolated chloroplasts were shown to convert [14C]phenylalanine and [14C]cinnamic acid into [14C]vanillin glucoside, indicating that the entire vanillin de novo biosynthetic machinery converting phenylalanine to vanillin glucoside is present in the chloroplast.

Today's status of application of high power electron beam welding to heavy electric machinery

International Nuclear Information System (INIS)

Kita, Hisanao; Okuni, Tetsuo; Sejima, Itsuhiko.

1980-01-01

The progress in high energy welding is remarkable in recent years, and electron beam welding is now widely used in heavy industries. However, there are number of problems to be solved in the application of high power electron beam welding to ultra thick steel plates (over 100 mm). The following matters are described: the economy of high power electron beam welding; the development of the welding machines; the problems in the actual application; the instances of the welding in a high-pressure spherical gas tank, non-magnetic steel structures and high-precision welded structures; weldor training; etc. For the future rise in the capacities of heavy electric machinery, the high efficiency by high power electron beam welding will be useful. The current status is its applications to the high-precision welding of large structures with 6 m diameter and the high-quality welding of heavy structures with 160 mm thickness. (J.P.N.)

The machinery of Nod-like receptors: refining the paths to immunity and cell death.

Science.gov (United States)

Saleh, Maya

2011-09-01

One of the fundamental aspects of the innate immune system is its capacity to discriminate between self and non-self or altered self, and to quickly respond by eliciting effector mechanisms that act in concert to restore normalcy. This capacity is determined by a set of evolutionarily conserved pattern recognition receptors (PRRs) that sense the presence of microbial motifs or endogenous danger signals, including tissue damage, cellular transformation or metabolic perturbation, and orchestrate the nature, duration and intensity of the innate immune response. Nod-like receptors (NLRs), a group of intracellular PRRs, are particularly essential as evident by the high incidence of genetic variations in their genes in various diseases of homeostasis. Here, I overview the signaling mechanisms of NLRs and discuss the mounting evidence of evolutionary conservation between their pathways and the cell death machinery. I also describe their effector functions that link the sensing of danger to the induction of inflammation, autophagy or cell death. © 2011 John Wiley & Sons A/S.

PRRT2 Is a Key Component of the Ca2+-Dependent Neurotransmitter Release Machinery

Science.gov (United States)

Valente, Pierluigi; Castroflorio, Enrico; Rossi, Pia; Fadda, Manuela; Sterlini, Bruno; Cervigni, Romina Ines; Prestigio, Cosimo; Giovedì, Silvia; Onofri, Franco; Mura, Elisa; Guarnieri, Fabrizia C.; Marte, Antonella; Orlando, Marta; Zara, Federico; Fassio, Anna; Valtorta, Flavia; Baldelli, Pietro; Corradi, Anna; Benfenati, Fabio

2016-01-01

Summary Heterozygous mutations in proline-rich transmembrane protein 2 (PRRT2) underlie a group of paroxysmal disorders, including epilepsy, kinesigenic dyskinesia, and migraine. Most of the mutations lead to impaired PRRT2 expression, suggesting that loss of PRRT2 function may contribute to pathogenesis. We show that PRRT2 is enriched in presynaptic terminals and that its silencing decreases the number of synapses and increases the number of docked synaptic vesicles at rest. PRRT2-silenced neurons exhibit a severe impairment of synchronous release, attributable to a sharp decrease in release probability and Ca2+ sensitivity and associated with a marked increase of the asynchronous/synchronous release ratio. PRRT2 interacts with the synaptic proteins SNAP-25 and synaptotagmin 1/2. The results indicate that PRRT2 is intimately connected with the Ca2+-sensing machinery and that it plays an important role in the final steps of neurotransmitter release. PMID:27052163

PRRT2 Is a Key Component of the Ca2+-Dependent Neurotransmitter Release Machinery

Directory of Open Access Journals (Sweden)

Pierluigi Valente

2016-04-01

Full Text Available Heterozygous mutations in proline-rich transmembrane protein 2 (PRRT2 underlie a group of paroxysmal disorders, including epilepsy, kinesigenic dyskinesia, and migraine. Most of the mutations lead to impaired PRRT2 expression, suggesting that loss of PRRT2 function may contribute to pathogenesis. We show that PRRT2 is enriched in presynaptic terminals and that its silencing decreases the number of synapses and increases the number of docked synaptic vesicles at rest. PRRT2-silenced neurons exhibit a severe impairment of synchronous release, attributable to a sharp decrease in release probability and Ca2+ sensitivity and associated with a marked increase of the asynchronous/synchronous release ratio. PRRT2 interacts with the synaptic proteins SNAP-25 and synaptotagmin 1/2. The results indicate that PRRT2 is intimately connected with the Ca2+-sensing machinery and that it plays an important role in the final steps of neurotransmitter release.

港口管理港口流动机械设备轮胎运营管理%Mnanagement of Tyre for Mobile Machinery Equipemnt of Port

Institute of Scientific and Technical Information of China (English)

王乾

2011-01-01

It is useful to enterprise for reducing cost and enhancing to strengthening management of tyre for mobile machinery equipment of port. This paper analyzes the difficulties in the running management. Then it puts forward measures from 6 aspects of the standard and profesional management of tyre of mobile machinery equipment, which has got good effect.%对港口流动机械轮胎加强管理有助于企业降低成本，提高效益。分析了大连港湾集装箱码头目前流动机械设备轮胎运营管理面临的困难，从6个方面提出了流动机械设备轮胎规范化、专业化管理的措施，取得了很好的效果。

Dengue Virus Selectively Annexes Endoplasmic Reticulum-Associated Translation Machinery as a Strategy for Co-opting Host Cell Protein Synthesis.

Science.gov (United States)

Reid, David W; Campos, Rafael K; Child, Jessica R; Zheng, Tianli; Chan, Kitti Wing Ki; Bradrick, Shelton S; Vasudevan, Subhash G; Garcia-Blanco, Mariano A; Nicchitta, Christopher V

2018-04-01

A primary question in dengue virus (DENV) biology is the molecular strategy for recruitment of host cell protein synthesis machinery. Here, we combined cell fractionation, ribosome profiling, and transcriptome sequencing (RNA-seq) to investigate the subcellular organization of viral genome translation and replication as well as host cell translation and its response to DENV infection. We report that throughout the viral life cycle, DENV plus- and minus-strand RNAs were highly partitioned to the endoplasmic reticulum (ER), identifying the ER as the primary site of DENV translation. DENV infection was accompanied by an ER compartment-specific remodeling of translation, where ER translation capacity was subverted from host transcripts to DENV plus-strand RNA, particularly at late stages of infection. Remarkably, translation levels and patterns in the cytosol compartment were only modestly affected throughout the experimental time course of infection. Comparisons of ribosome footprinting densities of the DENV plus-strand RNA and host mRNAs indicated that DENV plus-strand RNA was only sparsely loaded with ribosomes. Combined, these observations suggest a mechanism where ER-localized translation and translational control mechanisms, likely cis encoded, are used to repurpose the ER for DENV virion production. Consistent with this view, we found ER-linked cellular stress response pathways commonly associated with viral infection, namely, the interferon response and unfolded protein response, to be only modestly activated during DENV infection. These data support a model where DENV reprograms the ER protein synthesis and processing environment to promote viral survival and replication while minimizing the activation of antiviral and proteostatic stress response pathways. IMPORTANCE DENV, a prominent human health threat with no broadly effective or specific treatment, depends on host cell translation machinery for viral replication, immune evasion, and virion biogenesis. The

76 FR 30098 - Notice of Petitions by Firms for Determination of Eligibility To Apply for Trade Adjustment...

Science.gov (United States)

2011-05-24

.... Quality Casting and Aluminum 324 Hill Road, 11-May-11 The firm manufactures Products (QCAP). Franklin, NH 03235. products of aluminum, bronze, and zinc alloys using high-quality sand casting. Sauder Woodworking...

The RNAi machinery controls distinct responses to environmental signals in the basal fungus Mucor circinelloides.

Science.gov (United States)

Nicolás, Francisco E; Vila, Ana; Moxon, Simon; Cascales, María D; Torres-Martínez, Santiago; Ruiz-Vázquez, Rosa M; Garre, Victoriano

2015-03-25

RNA interference (RNAi) is a conserved mechanism of genome defence that can also have a role in the regulation of endogenous functions through endogenous small RNAs (esRNAs). In fungi, knowledge of the functions regulated by esRNAs has been hampered by lack of clear phenotypes in most mutants affected in the RNAi machinery. Mutants of Mucor circinelloides affected in RNAi genes show defects in physiological and developmental processes, thus making Mucor an outstanding fungal model for studying endogenous functions regulated by RNAi. Some classes of Mucor esRNAs map to exons (ex-siRNAs) and regulate expression of the genes from which they derive. To have a broad picture of genes regulated by the silencing machinery during vegetative growth, we have sequenced and compared the mRNA profiles of mutants in the main RNAi genes by using RNA-seq. In addition, we have achieved a more complete phenotypic characterization of silencing mutants. Deletion of any main RNAi gene provoked a deep impact in mRNA accumulation at exponential and stationary growth. Genes showing increased mRNA levels, as expected for direct ex-siRNAs targets, but also genes with decreased expression were detected, suggesting that, most probably, the initial ex-siRNA targets regulate the expression of other genes, which can be up- or down-regulated. Expression of 50% of the genes was dependent on more than one RNAi gene in agreement with the existence of several classes of ex-siRNAs produced by different combinations of RNAi proteins. These combinations of proteins have also been involved in the regulation of different cellular processes. Besides genes regulated by the canonical RNAi pathway, this analysis identified processes, such as growth at low pH and sexual interaction that are regulated by a dicer-independent non-canonical RNAi pathway. This work shows that the RNAi pathways play a relevant role in the regulation of a significant number of endogenous genes in M. circinelloides during exponential

A methodology for determining and controlling the buffers before floating bottlenecks in heavy machinery production

Directory of Open Access Journals (Sweden)

R. Lenort

2013-07-01

Full Text Available Heavy machinery industry is characterized by a number of specific features that cause significant variations in the processing time of products in the individual workplaces and frequent occurrence of floating bottlenecks, which change their positions. Depending on the product range being processed, a given workplace is the bottleneck only for some period of time. When the bottleneck moves to another workplace, it leads to unnecessary loss of capacity of the floating bottleneck. To maximize the utilization, it is necessary to protect those bottlenecks by creating special buffers. The objective of this article is to design a methodology used for the determination and control of buffers that are going to protect the floating bottlenecks from operating capacity losses caused by transfer of the constrain to another workplace. These buffers are referred to as „power buffers“. The designed methodology has been verified in the process of forged pieces machining.

Analysis of occupational accidents: prevention through the use of additional technical safety measures for machinery.

Science.gov (United States)

Dźwiarek, Marek; Latała, Agata

2016-01-01

This article presents an analysis of results of 1035 serious and 341 minor accidents recorded by Poland's National Labour Inspectorate (PIP) in 2005-2011, in view of their prevention by means of additional safety measures applied by machinery users. Since the analysis aimed at formulating principles for the application of technical safety measures, the analysed accidents should bear additional attributes: the type of machine operation, technical safety measures and the type of events causing injuries. The analysis proved that the executed tasks and injury-causing events were closely connected and there was a relation between casualty events and technical safety measures. In the case of tasks consisting of manual feeding and collecting materials, the injuries usually occur because of the rotating motion of tools or crushing due to a closing motion. Numerous accidents also happened in the course of supporting actions, like removing pollutants, correcting material position, cleaning, etc.

A Lagrangian parcel based mixing plane method for calculating water based mixed phase particle flows in turbo-machinery

Science.gov (United States)

Bidwell, Colin S.

2015-05-01

A method for calculating particle transport through turbo-machinery using the mixing plane analogy was developed and used to analyze the energy efficient engine . This method allows the prediction of temperature and phase change of water based particles along their path and the impingement efficiency and particle impact property data on various components in the engine. This methodology was incorporated into the LEWICE3D V3.5 software. The method was used to predict particle transport in the low pressure compressor of the . The was developed by NASA and GE in the early 1980s as a technology demonstrator and is representative of a modern high bypass turbofan engine. The flow field was calculated using the NASA Glenn ADPAC turbo-machinery flow solver. Computations were performed for a Mach 0.8 cruise condition at 11,887 m assuming a standard warm day for ice particle sizes of 5, 20 and 100 microns and a free stream particle concentration of . The impingement efficiency results showed that as particle size increased average impingement efficiencies and scoop factors increased for the various components. The particle analysis also showed that the amount of mass entering the inner core decreased with increased particle size because the larger particles were less able to negotiate the turn into the inner core due to particle inertia. The particle phase change analysis results showed that the larger particles warmed less as they were transported through the low pressure compressor. Only the smallest 5 micron particles were warmed enough to produce melting with a maximum average melting fraction of 0.18. The results also showed an appreciable amount of particle sublimation and evaporation for the 5 micron particles entering the engine core (22.6 %).

Decreased expression of RNA interference machinery, Dicer and Drosha, is associated with poor outcome in ovarian cancer patients

Energy Technology Data Exchange (ETDEWEB)

Merritt, William M.; Lin, Yvonne G.; Han, Liz Y.; Kamat, Aparna A.; Spannuth, Whitney A.; Schmandt, Rosemarie; Urbauer, Diana; Pennacchio, Len A.; Cheng, Jan-Fang; Zeidan, Alexandra; Wang, Hua; Mueller, Peter; Lenburg, Marc E.; Gray, Joe W.; Mok, Samuel; Birrer, Michael J.; Lopez-Berestein, Gabriel; Coleman, Robert L.; Bar-Eli, Menashe; Sood, Anil K.

2008-05-06

The clinical and functional significance of RNA interference (RNAi) machinery, Dicer and Drosha, in ovarian cancer is not known and was examined. Dicer and Drosha expression was measured in ovarian cancer cell lines (n=8) and invasive epithelial ovarian cancer specimens (n=111) and correlated with clinical outcome. Validation was performed with previously published cohorts of ovarian, breast, and lung cancer patients. Anti-Galectin-3 siRNA and shRNA transfections were used for in vitro functional studies. Dicer and Drosha mRNA and protein levels were decreased in 37% to 63% of ovarian cancer cell lines and in 60% and 51% of human ovarian cancer specimens, respectively. Low Dicer was significantly associated with advanced tumor stage (p=0.007), and low Drosha with suboptimal surgical cytoreduction (p=0.02). Tumors with both high Dicer and Drosha were associated with increased median patient survival (>11 years vs. 2.66 years for other groups; p<0.001). In multivariate analysis, high Dicer (HR=0.48; p=0.02), high-grade histology (HR=2.46; p=0.03), and poor chemoresponse (HR=3.95; p<0.001) were identified as independent predictors of disease-specific survival. Findings of poor clinical outcome with low Dicer expression were validated in separate cohorts of cancer patients. Galectin-3 silencing with siRNA transfection was superior to shRNA in cell lines with low Dicer (78-95% vs. 4-8% compared to non-targeting sequences), and similar in cell lines with high Dicer. Our findings demonstrate the clinical and functional impact of RNAi machinery alterations in ovarian carcinoma and support the use of siRNA constructs that do not require endogenous Dicer and Drosha for therapeutic applications.

The Saccharomyces cerevisiae MUM2 gene interacts with the DNA replication machinery and is required for meiotic levels of double strand breaks.

Science.gov (United States)

Davis, L; Barbera, M; McDonnell, A; McIntyre, K; Sternglanz, R; Jin , Q; Loidl, J; Engebrecht, J

2001-01-01

The Saccharomyces cerevisiae MUM2 gene is essential for meiotic, but not mitotic, DNA replication and thus sporulation. Genetic interactions between MUM2 and a component of the origin recognition complex and polymerase alpha-primase suggest that MUM2 influences the function of the DNA replication machinery. Early meiotic gene expression is induced to a much greater extent in mum2 cells than in meiotic cells treated with the DNA synthesis inhibitor hydroxyurea. This result indicates that the mum2 meiotic arrest is downstream of the arrest induced by hydroxyurea and suggests that DNA synthesis is initiated in the mutant. Genetic analyses indicate that the recombination that occurs in mum2 mutants is dependent on the normal recombination machinery and on synaptonemal complex components and therefore is not a consequence of lesions created by incompletely replicated DNA. Both meiotic ectopic and allelic recombination are similarly reduced in the mum2 mutant, and the levels are consistent with the levels of meiosis-specific DSBs that are generated. Cytological analyses of mum2 mutants show that chromosome pairing and synapsis occur, although at reduced levels compared to wild type. Given the near-wild-type levels of meiotic gene expression, pairing, and synapsis, we suggest that the reduction in DNA replication is directly responsible for the reduced level of DSBs and meiotic recombination. PMID:11238403

Modeling integrated cellular machinery using hybrid Petri-Boolean networks.

Directory of Open Access Journals (Sweden)

Natalie Berestovsky

Full Text Available The behavior and phenotypic changes of cells are governed by a cellular circuitry that represents a set of biochemical reactions. Based on biological functions, this circuitry is divided into three types of networks, each encoding for a major biological process: signal transduction, transcription regulation, and metabolism. This division has generally enabled taming computational complexity dealing with the entire system, allowed for using modeling techniques that are specific to each of the components, and achieved separation of the different time scales at which reactions in each of the three networks occur. Nonetheless, with this division comes loss of information and power needed to elucidate certain cellular phenomena. Within the cell, these three types of networks work in tandem, and each produces signals and/or substances that are used by the others to process information and operate normally. Therefore, computational techniques for modeling integrated cellular machinery are needed. In this work, we propose an integrated hybrid model (IHM that combines Petri nets and Boolean networks to model integrated cellular networks. Coupled with a stochastic simulation mechanism, the model simulates the dynamics of the integrated network, and can be perturbed to generate testable hypotheses. Our model is qualitative and is mostly built upon knowledge from the literature and requires fine-tuning of very few parameters. We validated our model on two systems: the transcriptional regulation of glucose metabolism in human cells, and cellular osmoregulation in S. cerevisiae. The model produced results that are in very good agreement with experimental data, and produces valid hypotheses. The abstract nature of our model and the ease of its construction makes it a very good candidate for modeling integrated networks from qualitative data. The results it produces can guide the practitioner to zoom into components and interconnections and investigate them

Exploiting immune cell metabolic machinery for functional HIV cure and the prevention of inflammaging [version 1; referees: 4 approved

Directory of Open Access Journals (Sweden)

Clovis S. Palmer

2018-01-01

Full Text Available An emerging paradigm in immunology suggests that metabolic reprogramming and immune cell activation and functions are intricately linked. Viral infections, such as HIV infection, as well as cancer force immune cells to undergo major metabolic challenges. Cells must divert energy resources in order to mount an effective immune response. However, the fact that immune cells adopt specific metabolic programs to provide host defense against intracellular pathogens and how this metabolic shift impacts immune cell functions and the natural course of diseases have only recently been appreciated. A clearer insight into how these processes are inter-related will affect our understanding of several fundamental aspects of HIV persistence. Even in patients with long-term use of anti-retroviral therapies, HIV infection persists and continues to cause chronic immune activation and inflammation, ongoing and cumulative damage to multiple organs systems, and a reduction in life expectancy. HIV-associated fundamental changes to the metabolic machinery of the immune system can promote a state of “inflammaging”, a chronic, low-grade inflammation with specific immune changes that characterize aging, and can also contribute to the persistence of HIV in its reservoirs. In this commentary, we will bring into focus evolving concepts on how HIV modulates the metabolic machinery of immune cells in order to persist in reservoirs and how metabolic reprogramming facilitates a chronic state of inflammation that underlies the development of age-related comorbidities. We will discuss how immunometabolism is facilitating the changing paradigms in HIV cure research and outline the novel therapeutic opportunities for preventing inflammaging and premature development of age-related conditions in HIV+ individuals.

Deep neural networks: A promising tool for fault characteristic mining and intelligent diagnosis of rotating machinery with massive data

Science.gov (United States)

Jia, Feng; Lei, Yaguo; Lin, Jing; Zhou, Xin; Lu, Na

2016-05-01

Aiming to promptly process the massive fault data and automatically provide accurate diagnosis results, numerous studies have been conducted on intelligent fault diagnosis of rotating machinery. Among these studies, the methods based on artificial neural networks (ANNs) are commonly used, which employ signal processing techniques for extracting features and further input the features to ANNs for classifying faults. Though these methods did work in intelligent fault diagnosis of rotating machinery, they still have two deficiencies. (1) The features are manually extracted depending on much prior knowledge about signal processing techniques and diagnostic expertise. In addition, these manual features are extracted according to a specific diagnosis issue and probably unsuitable for other issues. (2) The ANNs adopted in these methods have shallow architectures, which limits the capacity of ANNs to learn the complex non-linear relationships in fault diagnosis issues. As a breakthrough in artificial intelligence, deep learning holds the potential to overcome the aforementioned deficiencies. Through deep learning, deep neural networks (DNNs) with deep architectures, instead of shallow ones, could be established to mine the useful information from raw data and approximate complex non-linear functions. Based on DNNs, a novel intelligent method is proposed in this paper to overcome the deficiencies of the aforementioned intelligent diagnosis methods. The effectiveness of the proposed method is validated using datasets from rolling element bearings and planetary gearboxes. These datasets contain massive measured signals involving different health conditions under various operating conditions. The diagnosis results show that the proposed method is able to not only adaptively mine available fault characteristics from the measured signals, but also obtain superior diagnosis accuracy compared with the existing methods.

General Information about Paranasal Sinus and Nasal Cavity Cancer

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... such as those found in the following jobs: Furniture-making. Sawmill work. Woodworking (carpentry). Shoemaking. Metal-plating. ... cancer treatment that uses drugs to stop the growth of cancer cells, either by killing the cells ...

Hypersensitivity Pneumonitis

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... breed animals or birds, cheese washers, woodworkers, and wine makers—have a greater chance of exposure to ... this test, talk to your doctor about the benefits and possible risks of this procedure. Lung biopsies ...

Modeling of turbulent flows in cooling channels of turbo-machineries; Modelisation des ecoulements turbulents dans des canaux de refroidissement de turbomachines

Energy Technology Data Exchange (ETDEWEB)

Bidart, A.; Caltagirone, J.P.; Parneix, S. [Laboratoire MASTER-ENSCPB, 33 - Talence (France)

1997-12-31

The MASTER laboratory has been involved since several years in the creation and utilization of modeling tools for the prediction of 3-D turbulent flows and heat transfers in turbine blades in order to optimize the cooling systems of turbo-machineries. This paper describes one of the test-cases that has been used for the validation of the `Aquilon` calculation code developed in this aim. Then, the modeling performed with the `Fluent` industrial code in order to evaluate the possible improvements of the Aquilon code, is presented. (J.S.) 5 refs.

What's your relationship with computerized manufacturing technologies -- functional, dysfunctional or non-existent?

Science.gov (United States)

Jan Wiedenbeck; Jeff Parsons; Bruce Beeken

2009-01-01

Computer-aided manufacturing (CAM), in which computer-aided design (CAD) and computer numerically controlled (CNC) machining are integrated for the production of parts, became a viable option for the woodworking industry in the 1980s.

Dealing with the Effects of Trauma: A Self-Help Guide

Science.gov (United States)

... painting, drawing, woodworking, making a sculpture, reading fiction, comics, mystery novels, or inspira- tional writings, doing crossword ... for yourself. If you want to get more education for yourself so you can do work that ...

Arabidopsis TCP Transcription Factors Interact with the SUMO Conjugating Machinery in Nuclear Foci

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Magdalena J. Mazur

2017-11-01

Full Text Available In Arabidopsis more than 400 proteins have been identified as SUMO targets, both in vivo and in vitro. Among others, transcription factors (TFs are common targets for SUMO conjugation. Here we aimed to exhaustively screen for TFs that interact with the SUMO machinery using an arrayed yeast two-hybrid library containing more than 1,100 TFs. We identified 76 interactors that foremost interact with the SUMO conjugation enzyme SCE1 and/or the SUMO E3 ligase SIZ1. These interactors belong to various TF families, which control a wide range of processes in plant development and stress signaling. Amongst these interactors, the TCP family was overrepresented with several TCPs interacting with different proteins of the SUMO conjugation cycle. For a subset of these TCPs we confirmed that the catalytic site of SCE1 is essential for this interaction. In agreement, TCP1, TCP3, TCP8, TCP14, and TCP15 were readily SUMO modified in an E. coli sumoylation assay. Strikingly, these TCP-SCE1 interactions were found to redistribute these TCPs into nuclear foci/speckles, suggesting that these TCP foci represent sites for SUMO (conjugation activity.

Application of the international guidelines for machinery breakdown prevention at nuclear power plants

International Nuclear Information System (INIS)

Wendland, W.G.

2001-01-01

For more than forty years as a specialized branch of the worldwide insurance industry, the nuclear insurance pools have underwritten property damage protection for nuclear facilities throughout the world. At power plants insured by the pools, an enviable record of operational safety has been attained. Nevertheless, electrical and mechanical equipment does break down occasionally. Although these failures do not necessarily compromise nuclear safety, they can cause significant damage to equipment, leading to a considerable loss of generating revenue and causing sizeable insurance losses. Since insurance companies have a large financial stake in nuclear power plants, their goal is to minimize insurance losses, including the failure of systems and equipment and ensuing consequential damages. To ensure that the insurance risk is properly underwritten, insurance companies analyze loss information, develop loss prevention guidelines and focus loss control activities on those areas where insurance risk is most significant. This paper provides a chronology of the development of the ''International Guidelines for Machinery Breakdown Prevention at Nuclear Power Plants'' and describes the results of insurance inspections conducted using these guidelines. Included is a summary of guideline content and of insurance loss experience between 1962 and 1999. (author)

Analysis of occupational accidents: prevention through the use of additional technical safety measures for machinery

Science.gov (United States)

Dźwiarek, Marek; Latała, Agata

2016-01-01

This article presents an analysis of results of 1035 serious and 341 minor accidents recorded by Poland's National Labour Inspectorate (PIP) in 2005–2011, in view of their prevention by means of additional safety measures applied by machinery users. Since the analysis aimed at formulating principles for the application of technical safety measures, the analysed accidents should bear additional attributes: the type of machine operation, technical safety measures and the type of events causing injuries. The analysis proved that the executed tasks and injury-causing events were closely connected and there was a relation between casualty events and technical safety measures. In the case of tasks consisting of manual feeding and collecting materials, the injuries usually occur because of the rotating motion of tools or crushing due to a closing motion. Numerous accidents also happened in the course of supporting actions, like removing pollutants, correcting material position, cleaning, etc. PMID:26652689

Opposite roles of the Arabidopsis cytokinin receptors AHK2 and AHK3 in the expression of plastid genes and genes for the plastid transcriptional machinery during senescence.

Science.gov (United States)

Danilova, Maria N; Kudryakova, Natalia V; Doroshenko, Anastasia S; Zabrodin, Dmitry A; Rakhmankulova, Zulfira F; Oelmüller, Ralf; Kusnetsov, Victor V

2017-03-01

Cytokinin membrane receptors of the Arabidopsis thaliana AHK2 and AHK3 play opposite roles in the expression of plastid genes and genes for the plastid transcriptional machinery during leaf senescence Loss-of-function mutants of Arabidopsis thaliana were used to study the role of cytokinin receptors in the expression of chloroplast genes during leaf senescence. Accumulation of transcripts of several plastid-encoded genes is dependent on the АНК2/АНК3 receptor combination. АНК2 is particularly important at the final stage of plant development and, unlike АНК3, a positive regulator of leaf senescence. Cytokinin-dependent up-regulation of the nuclear encoded genes for chloroplast RNA polymerases RPOTp and RPOTmp suggests that the hormone controls plastid gene expression, at least in part, via the expression of nuclear genes for the plastid transcription machinery. This is further supported by cytokinin dependent regulation of genes for the nuclear encoded plastid σ-factors, SIG1-6, which code for components of the transcriptional apparatus in chloroplasts.

ANALISIS KUANTITATIF DAN KUALITATIF PENGENDALIAN BAHAYA TEMPAT KERJA DI PERUSAHAAN MACHINERY PT. X BEKASI

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Wahyu Setia Wisesa

2016-10-01

Full Text Available Perusahaan wajib menerapkan keselamatan dan kesehatan kerja, tetapi bagaimana manajemen dan karyawan dalam mempraktekan pengendalian bahaya di tempat kerja perlu dilakukan pengkajian. Tujuan penelitian ini adalah untuk mencari pengaruh pengendalian bahaya di tempat kerja secara kuantitatif pada perusahaan machinery PT. X Bekasi. Metode yang digunakan adalah kuesioner dan pengamatan lapangan dengan melibatkan 56 karyawan secara acak sebagai responden. Secara kuantitatif kuesioner diolah menggunakan uji statistik dengan SPSS versi 21 untuk mencari korelasi secara linier regresi sedangkan secara kualitatif pengamatan didokumentasikan berdasarkan kelengkapan penunjang pengendalian bahaya di tempat kerja. Hasil yang diperoleh menunjukkan adanya korelasi secara linier antara pengendalian bahaya di tempat kerja (Y, terhadap desain organisasi (X1, faktor pengendalian manusia (X2 dan pengendalian teknis (X3 dalam bentuk : Y= -5,355 + 1,037 . X1 + 0,996 . X2 + 1,017. X3 dengan koefisien determinasi = 0,994. Hasil pengamatan kualitatif di lapangan menunjukkan masih perlu peran aktif dari pihak manajemen perusahaan dan karyawan dalam menerapkan peraturan K3 serta mengendalikan bahaya di tempat kerja.

Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCID.

Science.gov (United States)

Sauer, Aisha V; Brigida, Immacolata; Carriglio, Nicola; Hernandez, Raisa Jofra; Scaramuzza, Samantha; Clavenna, Daniela; Sanvito, Francesca; Poliani, Pietro L; Gagliani, Nicola; Carlucci, Filippo; Tabucchi, Antonella; Roncarolo, Maria Grazia; Traggiai, Elisabetta; Villa, Anna; Aiuti, Alessandro

2012-02-09

Adenosine acts as anti-inflammatory mediator on the immune system and has been described in regulatory T cell (Treg)-mediated suppression. In the absence of adenosine deaminase (ADA), adenosine and other purine metabolites accumulate, leading to severe immunodeficiency with recurrent infections (ADA-SCID). Particularly ADA-deficient patients with late-onset forms and after enzyme replacement therapy (PEG-ADA) are known to manifest immune dysregulation. Herein we provide evidence that alterations in the purine metabolism interfere with Treg function, thereby contributing to autoimmune manifestations in ADA deficiency. Tregs isolated from PEG-ADA-treated patients are reduced in number and show decreased suppressive activity, whereas they are corrected after gene therapy. Untreated murine ADA(-/-) Tregs show alterations in the plasma membrane CD39/CD73 ectonucleotidase machinery and limited suppressive activity via extracellular adenosine. PEG-ADA-treated mice developed multiple autoantibodies and hypothyroidism in contrast to mice treated with bone marrow transplantation or gene therapy. Tregs isolated from PEG-ADA-treated mice lacked suppressive activity, suggesting that this treatment interferes with Treg functionality. The alterations in the CD39/CD73 adenosinergic machinery and loss of function in ADA-deficient Tregs provide new insights into a predisposition to autoimmunity and the underlying mechanisms causing defective peripheral tolerance in ADA-SCID.

Data Base Directions; the Next Steps. Proceedings of the Workshop of the National Bureau of Standards and the Association for Computing Machinery (Fort Lauderdale, Florida, October 29-31, 1975).

Science.gov (United States)

Berg, John L., Ed.

To investigate the information needs of managers making decisions regarding the use of data base technology, the National Bureau of Standards and the Association for Computing Machinery held a workshop with approximately 80 experts in five major areas: auditing, evolving technology, government regulation, standards, and user experience. Results of…

Hepatitis C Virus Proteins Interact with the Endosomal Sorting Complex Required for Transport (ESCRT Machinery via Ubiquitination To Facilitate Viral Envelopment

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Rina Barouch-Bentov

2016-11-01

Full Text Available Enveloped viruses commonly utilize late-domain motifs, sometimes cooperatively with ubiquitin, to hijack the endosomal sorting complex required for transport (ESCRT machinery for budding at the plasma membrane. However, the mechanisms underlying budding of viruses lacking defined late-domain motifs and budding into intracellular compartments are poorly characterized. Here, we map a network of hepatitis C virus (HCV protein interactions with the ESCRT machinery using a mammalian-cell-based protein interaction screen and reveal nine novel interactions. We identify HRS (hepatocyte growth factor-regulated tyrosine kinase substrate, an ESCRT-0 complex component, as an important entry point for HCV into the ESCRT pathway and validate its interactions with the HCV nonstructural (NS proteins NS2 and NS5A in HCV-infected cells. Infectivity assays indicate that HRS is an important factor for efficient HCV assembly. Specifically, by integrating capsid oligomerization assays, biophysical analysis of intracellular viral particles by continuous gradient centrifugations, proteolytic digestion protection, and RNase digestion protection assays, we show that HCV co-opts HRS to mediate a late assembly step, namely, envelopment. In the absence of defined late-domain motifs, K63-linked polyubiquitinated lysine residues in the HCV NS2 protein bind the HRS ubiquitin-interacting motif to facilitate assembly. Finally, ESCRT-III and VPS/VTA1 components are also recruited by HCV proteins to mediate assembly. These data uncover involvement of ESCRT proteins in intracellular budding of a virus lacking defined late-domain motifs and a novel mechanism by which HCV gains entry into the ESCRT network, with potential implications for other viruses.

Cancer incidence among workers exposed to softwood dust in Lithuania.

Science.gov (United States)

Smailyte, Giedre

2012-06-01

The aim of this study was to assess cancer incidence in a cohort of woodworkers exposed to softwood dust in a Lithuanian wooden joinery products factory. The study population consisted of 1518 workers (1080 men and 438 women) employed in the factory for at least 1 year between 1947 and 1996 and living in Lithuania on 1 January 1978, when the follow-up for cancer incidence began. The follow-up period for cancer was 1978-2007. Cancer risk was assessed by standardised incidence ratios (SIR) with reference to the national population. Overall cancer incidence was not increased among woodworkers. However, the number of mouth and pharynx cancer cases among male woodworkers was significantly increased compared with expected numbers (SIR 2.19, 95% CI 1.17 to 3.74). A higher risk was found for cancer of the buccal cavity than for pharyngeal cancer (SIRs 2.83 and 1.45, respectively). The SIR for larynx cancer was also elevated (SIR 1.39, 95% CI 0.64 to 2.64) among men, while the number of lung cancer cases was higher than expected only among women (SIR 2.07, 95% CI 00.57 to 5.31). This results of this study support the hypothesis that exposure to softwood dust may increase the risk of oral and pharyngeal cancer. No support was found for an increased risk of other respiratory cancers among workers exposed to softwood dust.

Development and demonstration of surveillance and diagnostics of rotating machinery for reducing radiation exposure to nuclear power plant personnel: Appendices: Final report

International Nuclear Information System (INIS)

Allen, J.W.; Bohanick, J.S.

1988-01-01

This program was designed to reduce radiation exposure to power plant personnel resulting from inspection, maintenance, and repair of rotating equipment. The new rotating machinery monitoring system for this program was installed at GGNS during August 1983. The following nine appendices are presented: signals monitored at GGNS; definition of characterized spectral values; instructions for alignment and balance programs; machine diagrams; FFT program; software module descriptions; sample radiation survey forms used for exposure study; radiation exposure compared to other plants; and a technical section instruction for the vibration monitoring program at GGNS

Three dimensional flow phenomena in fluid machinery; Proceedings of the winter annual meeting, Miami Beach, FL, November 17-22, 1985

International Nuclear Information System (INIS)

Hamed, A.; Herring, J.; Povinelli, L.

1985-01-01

Papers in this volume provide an overview of the latest developments in experimental measurements and analytical and numerical predictions of three-dimensional flows in fluid machinery. Topics discussed include three-dimensional cascade testing of turbine nozzles at high exit Mach number; the use of a secondary flow computation in the compressor design process; an experimental investigation of static propeller flow field; and calculation of three-dimensional boundary layers on rotating turbine blades. Papers are also presented on a three-dimensional solution method for turbomachinery analysis; analysis of rotational inviscid flows in curved passages; and a mathematical model for the analysis of fluid flow in a scroll

UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii

Science.gov (United States)

Allorent, Guillaume; Lefebvre-Legendre, Linnka; Chappuis, Richard; Kuntz, Marcel; Truong, Thuy B.; Niyogi, Krishna K.; Goldschmidt-Clermont, Michel

2016-01-01

Life on earth is dependent on the photosynthetic conversion of light energy into chemical energy. However, absorption of excess sunlight can damage the photosynthetic machinery and limit photosynthetic activity, thereby affecting growth and productivity. Photosynthetic light harvesting can be down-regulated by nonphotochemical quenching (NPQ). A major component of NPQ is qE (energy-dependent nonphotochemical quenching), which allows dissipation of light energy as heat. Photodamage peaks in the UV-B part of the spectrum, but whether and how UV-B induces qE are unknown. Plants are responsive to UV-B via the UVR8 photoreceptor. Here, we report in the green alga Chlamydomonas reinhardtii that UVR8 induces accumulation of specific members of the light-harvesting complex (LHC) superfamily that contribute to qE, in particular LHC Stress-Related 1 (LHCSR1) and Photosystem II Subunit S (PSBS). The capacity for qE is strongly induced by UV-B, although the patterns of qE-related proteins accumulating in response to UV-B or to high light are clearly different. The competence for qE induced by acclimation to UV-B markedly contributes to photoprotection upon subsequent exposure to high light. Our study reveals an anterograde link between photoreceptor-mediated signaling in the nucleocytosolic compartment and the photoprotective regulation of photosynthetic activity in the chloroplast. PMID:27930292

UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii.

Science.gov (United States)

Allorent, Guillaume; Lefebvre-Legendre, Linnka; Chappuis, Richard; Kuntz, Marcel; Truong, Thuy B; Niyogi, Krishna K; Ulm, Roman; Goldschmidt-Clermont, Michel

2016-12-20

Life on earth is dependent on the photosynthetic conversion of light energy into chemical energy. However, absorption of excess sunlight can damage the photosynthetic machinery and limit photosynthetic activity, thereby affecting growth and productivity. Photosynthetic light harvesting can be down-regulated by nonphotochemical quenching (NPQ). A major component of NPQ is qE (energy-dependent nonphotochemical quenching), which allows dissipation of light energy as heat. Photodamage peaks in the UV-B part of the spectrum, but whether and how UV-B induces qE are unknown. Plants are responsive to UV-B via the UVR8 photoreceptor. Here, we report in the green alga Chlamydomonas reinhardtii that UVR8 induces accumulation of specific members of the light-harvesting complex (LHC) superfamily that contribute to qE, in particular LHC Stress-Related 1 (LHCSR1) and Photosystem II Subunit S (PSBS). The capacity for qE is strongly induced by UV-B, although the patterns of qE-related proteins accumulating in response to UV-B or to high light are clearly different. The competence for qE induced by acclimation to UV-B markedly contributes to photoprotection upon subsequent exposure to high light. Our study reveals an anterograde link between photoreceptor-mediated signaling in the nucleocytosolic compartment and the photoprotective regulation of photosynthetic activity in the chloroplast.

Stac3 is a component of the excitation-contraction coupling machinery and mutated in Native American myopathy

Science.gov (United States)

Horstick, Eric J.; Linsley, Jeremy W.; Dowling, James J.; Hauser, Michael A.; McDonald, Kristin K.; Ashley-Koch, Allison; Saint-Amant, Louis; Satish, Akhila; Cui, Wilson W.; Zhou, Weibin; Sprague, Shawn M.; Stamm, Demetra S.; Powell, Cynthia M.; Speer, Marcy C.; Franzini-Armstrong, Clara; Hirata, Hiromi; Kuwada, John Y.

2013-01-01

Excitation-contraction coupling, the process that regulates contractions by skeletal muscles, transduces changes in membrane voltage by activating release of Ca2+ from internal stores to initiate muscle contraction. Defects in EC coupling are associated with muscle diseases. Here we identify Stac3 as a novel component of the EC coupling machinery. Using a zebrafish genetic screen, we generate a locomotor mutation that is mapped to stac3. We provide electrophysiological, Ca2+ imaging, immunocytochemical and biochemical evidence that Stac3 participates in excitation-contraction coupling in muscles. Furthermore, we reveal that a mutation in human STAC3 as the genetic basis of the debilitating Native American myopathy (NAM). Analysis of NAM stac3 in zebrafish shows that the NAM mutation decreases excitation-contraction coupling. These findings enhance our understanding of both excitation-contraction coupling and the pathology of myopathies. PMID:23736855

Core RNAi machinery and gene knockdown in the emerald ash borer (Agrilus planipennis).

Science.gov (United States)

Zhao, Chaoyang; Alvarez Gonzales, Miguel A; Poland, Therese M; Mittapalli, Omprakash

2015-01-01

The RNA interference (RNAi) technology has been widely used in insect functional genomics research and provides an alternative approach for insect pest management. To understand whether the emerald ash borer (Agrilus planipennis), an invasive and destructive coleopteran insect pest of ash tree (Fraxinus spp.), possesses a strong RNAi machinery that is capable of degrading target mRNA as a response to exogenous double-stranded RNA (dsRNA) induction, we identified three RNAi pathway core component genes, Dicer-2, Argonaute-2 and R2D2, from the A. planipennis genome sequence. Characterization of these core components revealed that they contain conserved domains essential for the proteins to function in the RNAi pathway. Phylogenetic analyses showed that they are closely related to homologs derived from other coleopteran species. We also delivered the dsRNA fragment of AplaScrB-2, a β-fructofuranosidase-encoding gene horizontally acquired by A. planipennis as we reported previously, into A. planipennis adults through microinjection. Quantitative real-time PCR analysis on the dsRNA-treated beetles demonstrated a significantly decreased gene expression level of AplaScrB-2 appearing on day 2 and lasting until at least day 6. This study is the first record of RNAi applied in A. planipennis. Copyright © 2015 Elsevier Ltd. All rights reserved.

Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCID

Science.gov (United States)

Sauer, Aisha V.; Brigida, Immacolata; Carriglio, Nicola; Jofra Hernandez, Raisa; Scaramuzza, Samantha; Clavenna, Daniela; Sanvito, Francesca; Poliani, Pietro L.; Gagliani, Nicola; Carlucci, Filippo; Tabucchi, Antonella; Roncarolo, Maria Grazia; Traggiai, Elisabetta; Villa, Anna

2012-01-01

Adenosine acts as anti-inflammatory mediator on the immune system and has been described in regulatory T cell (Treg)–mediated suppression. In the absence of adenosine deaminase (ADA), adenosine and other purine metabolites accumulate, leading to severe immunodeficiency with recurrent infections (ADA-SCID). Particularly ADA-deficient patients with late-onset forms and after enzyme replacement therapy (PEG-ADA) are known to manifest immune dysregulation. Herein we provide evidence that alterations in the purine metabolism interfere with Treg function, thereby contributing to autoimmune manifestations in ADA deficiency. Tregs isolated from PEG-ADA–treated patients are reduced in number and show decreased suppressive activity, whereas they are corrected after gene therapy. Untreated murine ADA−/− Tregs show alterations in the plasma membrane CD39/CD73 ectonucleotidase machinery and limited suppressive activity via extracellular adenosine. PEG-ADA–treated mice developed multiple autoantibodies and hypothyroidism in contrast to mice treated with bone marrow transplantation or gene therapy. Tregs isolated from PEG-ADA–treated mice lacked suppressive activity, suggesting that this treatment interferes with Treg functionality. The alterations in the CD39/CD73 adenosinergic machinery and loss of function in ADA-deficient Tregs provide new insights into a predisposition to autoimmunity and the underlying mechanisms causing defective peripheral tolerance in ADA-SCID. Trials were registered at www.clinicaltrials.gov as NCT00598481/NCT00599781. PMID:22184407

Fault Diagnosis of Rotating Machinery Based on Multisensor Information Fusion Using SVM and Time-Domain Features

Directory of Open Access Journals (Sweden)

Ling-li Jiang

2014-01-01

Full Text Available Multisensor information fusion, when applied to fault diagnosis, the time-space scope, and the quantity of information are expanded compared to what could be acquired by a single sensor, so the diagnostic object can be described more comprehensively. This paper presents a methodology of fault diagnosis in rotating machinery using multisensor information fusion that all the features are calculated using vibration data in time domain to constitute fusional vector and the support vector machine (SVM is used for classification. The effectiveness of the presented methodology is tested by three case studies: diagnostic of faulty gear, rolling bearing, and identification of rotor crack. For each case study, the sensibilities of the features are analyzed. The results indicate that the peak factor is the most sensitive feature in the twelve time-domain features for identifying gear defect, and the mean, amplitude square, root mean square, root amplitude, and standard deviation are all sensitive for identifying gear, rolling bearing, and rotor crack defect comparatively.

Flow-Induced Pulsation and Vibration in Hydroelectric Machinery Engineer’s Guidebook for Planning, Design and Troubleshooting

CERN Document Server

Dörfler, Peter; Coutu, André

2013-01-01

Since the 1970’s, an increasing amount of specialized research has focused on the problems created by instability of internal flow in hydroelectric power plants. However, progress in this field is hampered by the inter­disciplinary nature of the subject, between fluid mechanics, structural mechanics and hydraulic transients. Flow-induced Pulsation and Vibration in Hydroelectric Machinery provides a compact guidebook explaining the many different underlying physical mechanisms and their possible effects.   Typical phenomena are described to assist in the proper diagnosis of problems and various key strategies for solution are compared and considered with support from practical experience and real-life examples. The link between state-of the-art CFD computation and notorious practical problems is discussed  and quantitative data is provided on  normal levels of vibration and pulsation so realistic limits can be set for future projects. Current projects are also addressed as the possibilities and limitatio...

Improved hybrid isolator with maglev actuator integrated in air spring for active-passive isolation of ship machinery vibration

Science.gov (United States)

Li, Yan; He, Lin; Shuai, Chang-geng; Wang, Chun-yu

2017-10-01

A hybrid isolator consisting of maglev actuator and air spring is proposed and developed for application in active-passive vibration isolation system of ship machinery. The dynamic characteristics of this hybrid isolator are analyzed and tested. The stability and adaptability of this hybrid isolator to shock and swing in the marine environment are improved by a compliant gap protection technique and a disengageable suspended structure. The functions of these new engineering designs are proved by analytical verification and experimental validation of the designed stiffness of such a hybrid isolator, and also by shock adaptability testing of the hybrid isolator. Finally, such hybrid isolators are installed in an engineering mounting loaded with a 200-kW ship diesel generator, and the broadband and low-frequency sinusoidal isolation performance is tested.

TAC102 Is a Novel Component of the Mitochondrial Genome Segregation Machinery in Trypanosomes.

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Roman Trikin

2016-05-01

Full Text Available Trypanosomes show an intriguing organization of their mitochondrial DNA into a catenated network, the kinetoplast DNA (kDNA. While more than 30 proteins involved in kDNA replication have been described, only few components of kDNA segregation machinery are currently known. Electron microscopy studies identified a high-order structure, the tripartite attachment complex (TAC, linking the basal body of the flagellum via the mitochondrial membranes to the kDNA. Here we describe TAC102, a novel core component of the TAC, which is essential for proper kDNA segregation during cell division. Loss of TAC102 leads to mitochondrial genome missegregation but has no impact on proper organelle biogenesis and segregation. The protein is present throughout the cell cycle and is assembled into the newly developing TAC only after the pro-basal body has matured indicating a hierarchy in the assembly process. Furthermore, we provide evidence that the TAC is replicated de novo rather than using a semi-conservative mechanism. Lastly, we demonstrate that TAC102 lacks an N-terminal mitochondrial targeting sequence and requires sequences in the C-terminal part of the protein for its proper localization.

Integration of Signaling Pathways with the Epigenetic Machinery in the Maintenance of Stem Cells

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Luca Fagnocchi

2016-01-01

Full Text Available Stem cells balance their self-renewal and differentiation potential by integrating environmental signals with the transcriptional regulatory network. The maintenance of cell identity and/or cell lineage commitment relies on the interplay of multiple factors including signaling pathways, transcription factors, and the epigenetic machinery. These regulatory modules are strongly interconnected and they influence the pattern of gene expression of stem cells, thus guiding their cellular fate. Embryonic stem cells (ESCs represent an invaluable tool to study this interplay, being able to indefinitely self-renew and to differentiate towards all three embryonic germ layers in response to developmental cues. In this review, we highlight those mechanisms of signaling to chromatin, which regulate chromatin modifying enzymes, histone modifications, and nucleosome occupancy. In addition, we report the molecular mechanisms through which signaling pathways affect both the epigenetic and the transcriptional state of ESCs, thereby influencing their cell identity. We propose that the dynamic nature of oscillating signaling and the different regulatory network topologies through which those signals are encoded determine specific gene expression programs, leading to the fluctuation of ESCs among multiple pluripotent states or to the establishment of the necessary conditions to exit pluripotency.

Machinery Fault Diagnosis Using Two-Channel Analysis Method Based on Fictitious System Frequency Response Function

Directory of Open Access Journals (Sweden)

Kihong Shin

2015-01-01

Full Text Available Most existing techniques for machinery health monitoring that utilize measured vibration signals usually require measurement points to be as close as possible to the expected fault components of interest. This is particularly important for implementing condition-based maintenance since the incipient fault signal power may be too small to be detected if a sensor is located further away from the fault source. However, a measurement sensor is often not attached to the ideal point due to geometric or environmental restrictions. In such a case, many of the conventional diagnostic techniques may not be successfully applicable. In this paper, a two-channel analysis method is proposed to overcome such difficulty. It uses two vibration signals simultaneously measured at arbitrary points in a machine. The proposed method is described theoretically by introducing a fictitious system frequency response function. It is then verified experimentally for bearing fault detection. The results show that the suggested method may be a good alternative when ideal points for measurement sensors are not readily available.

Software para estimativa do custo operacional de máquinas agrícolas - MAQCONTROL Development of software to compute operational costs of farm machinery - MAQCONTROL

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Liane Piacentini

2012-06-01

Full Text Available A seleção e a otimização de sistemas mecanizados são os principais objetivos da mecanização racional. Não é suficiente uma compra adequada do maquinário agrícola se sua utilização não for controlada em aspectos operacionais e financeiros. Neste trabalho, é descrito o desenvolvimento de software para estimativa do custo operacional de máquinas agrícolas (MAQCONTROL, utilizando o ambiente de desenvolvimento Borland Delphi e o banco de dados Firebird. Os custos operacionais foram divididos em fixos e variáveis. Nos custos fixos, foram estimadas as despesas com depreciação, juros, alojamento e seguros. Nos custos variáveis, foi dada ênfase aos custos de manutenção como: óleos lubrificantes, filtros, pneus, graxa, combustível, pequenos reparos e troca de peças. Os resultados demonstraram a eficiência do software para os objetivos propostos. Assim, o MAQCONTROL pode ser uma importante ferramenta no processo de administração rural, pois reduz os custos da informação e agiliza a determinação precisa dos custos operacionais de máquinas agrícolas.The rational mechanization has as main objectives the selection and optimization of mechanized systems. An adequate purchase of agricultural machinery is not sufficient if its use is not controlled in operational and financial aspects. This work describes the development of software to estimate operational costs of agricultural machinery (MAQCONTROL, using Borland Delphi's development environment and Firebird database. The operational costs were divided in fixed and variable. In fixed costs, the expenses with depreciation, interest, storage and insurance were estimated. In variable costs, the emphasis was given to the expenses on maintenance, lubricating oils, filters, tires, grease, fuel, small repairs, and parts replacement. Results have shown the software efficiency for the proposed objectives. Therefore, the MAQCONTROL software proved to be an important tool in the rural

The effect of different workplace nanoparticles on the immune systems of employees

Science.gov (United States)

Kurjane, Natalja; Zvagule, Tija; Reste, Jelena; Martinsone, Zanna; Pavlovska, Ilona; Martinsone, Inese; Vanadzins, Ivars

2017-09-01

Currently, nanoparticles are widely present in the environment and are being used in various industrial technologies. Nanoparticles affect immune functions, causing different immune responses. The aim of the current study was to evaluate several cytokines, interleukin (IL)-1b, IL-6, IL-8, tumour necrosis factor-a (TNF-α), interferon-γ, adhesive molecule sICAM-1, macrophage inhibitory protein 1a (MIP1a) and secretory immunoglobulin A, in nasal lavage fluid and in the peripheral blood of healthy subjects exposed to workplace nanoparticles. Thirty-six employees from three different environments were examined: 12 from a metalworking company, 12 from a woodworking company and 12 office workers. The nanoparticles in the different workplaces were detected in the air in the immediate vicinity of the employees. The particle number concentration and surface area values were significantly higher in the workplaces of the metal- and woodworking industries, but concentrations of mass were lower (the measurements were performed by an electrical low-pressure impactor ELPI+). Energy dispersive X-ray spectroscopy (EDS, an attachment to a high-resolution SEM) was used to provide elemental analysis or chemical characterization of the dust particles in a low-vacuum field-free mode operating at a potential of 15 kV spot 3.0. The technique used provided quantitative and spatial analyses of the distribution of elements through mapping (two to three parallel measurements) and point analysis (four to five parallel measurements). Samples from the metal industry contained more ultramicroscopic and nanometric particles, e.g. toxic metals such as Zn, Mn and Cr, and fewer microscopic dust particles. The nasal lavage and peripheral blood were taken at the beginning and the end of the working week, when immune indices were measured. Our data showed a statistically significant increased level of the pro-inflammatory cytokine TNF-α in serum in both exposed groups compared with office workers as

Endosomal Escape and Delivery of CRISPR/Cas9 Genome Editing Machinery Enabled by Nanoscale Zeolitic Imidazolate Framework

KAUST Repository

Alsaiari, Shahad K.

2017-12-22

CRISPR/Cas9 is a combined protein (Cas9) and an engineered single guide RNA (sgRNA) genome editing platform that offers revolutionary solutions to genetic diseases. It has, however, a double delivery problem owning to the large protein size and the highly charged RNA component. In this work, we report the first example of CRISPR/Cas9 encapsulated by nanoscale zeolitic imidazole frameworks (ZIFs) with a loading efficiency of 17% and enhanced endosomal escape promoted by the protonated imidazole moieties. The gene editing potential of CRISPR/Cas9 encapsulated by ZIF-8 (CC-ZIFs) is further verified by knocking down the gene expression of green fluorescent protein by 37% over 4 days employing CRISPR/Cas9 machinery. The nanoscale CC-ZIFs are biocompatible and easily scaled-up offering excellent loading capacity and controlled co-delivery of intact Cas9 protein and sgRNA.

Endosomal Escape and Delivery of CRISPR/Cas9 Genome Editing Machinery Enabled by Nanoscale Zeolitic Imidazolate Framework

KAUST Repository

Alsaiari, Shahad K.; Patil, Sachin; Alyami, Mram Z.; Alamoudi, Kholod; Aleisa, Fajr A; Merzaban, Jasmeen; Li, Mo; Khashab, Niveen M.

2017-01-01

CRISPR/Cas9 is a combined protein (Cas9) and an engineered single guide RNA (sgRNA) genome editing platform that offers revolutionary solutions to genetic diseases. It has, however, a double delivery problem owning to the large protein size and the highly charged RNA component. In this work, we report the first example of CRISPR/Cas9 encapsulated by nanoscale zeolitic imidazole frameworks (ZIFs) with a loading efficiency of 17% and enhanced endosomal escape promoted by the protonated imidazole moieties. The gene editing potential of CRISPR/Cas9 encapsulated by ZIF-8 (CC-ZIFs) is further verified by knocking down the gene expression of green fluorescent protein by 37% over 4 days employing CRISPR/Cas9 machinery. The nanoscale CC-ZIFs are biocompatible and easily scaled-up offering excellent loading capacity and controlled co-delivery of intact Cas9 protein and sgRNA.

The dynamic nature and territory of transcriptional machinery in the bacterial chromosome

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Ding Jun Jin

2015-05-01

Full Text Available Our knowledge of the regulation of genes involved in bacterial growth and stress responses is extensive; however, we have only recently begun to understand how environmental cues influence the dynamic, three-dimensional distribution of RNA polymerase (RNAP in Escherichia coli on the level of single cell, using wide-field fluorescence microscopy and state-of-the-art imaging techniques. Live-cell imaging using either an agarose-embedding procedure or a microfluidic system further underscores the dynamic nature of the distribution of RNAP in response to changes in the environment. A general agreement between live-cell and fixed-cell images has validated the formaldehyde-fixing procedure, which is a technical breakthrough in the study of the cell biology of RNAP. In this review we use a systems biology perspective to summarize the advances in the cell biology of RNAP in E. coli, including the discoveries of the bacterial nucleolus, the spatial compartmentalization of the transcription machinery at the periphery of the nucleoid, and the segregation of the chromosome territories for the two major cellular functions of transcription and replication in fast-growing cells. Our understanding of the coupling of transcription and bacterial chromosome (or nucleoid structure is also summarized. Using E. coli as a simple model system, co-imaging of RNAP with DNA and other factors during growth and stress responses will continue to be a useful tool for studying bacterial growth and adaptation in changing environment.

Langerhans cells and subsets of lymphocytes in the nasal mucosa

DEFF Research Database (Denmark)

Hellquist-Dahl, B; Olsen, K E; Irander, K

1991-01-01

Langerhans cells and different lymphocytes were studied in the nasal mucosa of 39 woodwork teachers and a control group of 14 healthy subjects. Ten of the woodwork teachers were sensitized as determined by skin prick test. A panel of different monoclonal antibodies was applied on the frozen nasal...... mucosal specimens. Intraepithelial CD1-positive dendritic cells were found in all specimens. However, there was no difference between the number of these Langerhans cells found in the study group and the number found in the controls. In every specimen the intraepithelial lymphocyte population...... was dominated by T lymphocytes, and there were relatively few B cells. Similarly the ratio between CD4- and CD8-positive lymphocytes in the study group and the controls was the same. In all specimens there was a dominance of T suppressor/cytotoxic cells compared with T helper/inducer cells. The study confirms...

Monitoring of a micro-smart grid: Power consumption data of some machineries of an agro-industrial test site.

Science.gov (United States)

Fabrizio, Enrico; Biglia, Alessandro; Branciforti, Valeria; Filippi, Marco; Barbero, Silvia; Tecco, Giuseppe; Mollo, Paolo; Molino, Andrea

2017-02-01

For the management of a (micro)-smart grid it is important to know the patters of the load profiles and of the generators. In this article the power consumption data obtained through a monitoring activity developed on a micro-smart grid in an agro-industrial test-site are presented. In particular, this reports the synthesis of the monitoring results of 5 loads (5 industrial machineries for crop micronization, corncob crashing and other similar processes). How these data were used within a monitoring and managing scheme of a micro-smart grid can be found in (E. Fabrizio, V. Branciforti, A. Costantino, M. Filippi, S. Barbero, G. Tecco, P. Mollo, A. Molino, 2017) [1]. The data can be useful for other researchers in order to create benchmarks of energy use input appropriate energy demand values in optimization tools for the industrial sector.

The effects of environmental chemical carcinogens on the microRNA machinery.

Science.gov (United States)

Izzotti, A; Pulliero, A

2014-07-01

The first evidence that microRNA expression is early altered by exposure to environmental chemical carcinogens in still healthy organisms was obtained for cigarette smoke. To date, the cumulative experimental data indicate that similar effects are caused by a variety of environmental carcinogens, including polycyclic aromatic hydrocarbons, nitropyrenes, endocrine disruptors, airborne mixtures, carcinogens in food and water, and carcinogenic drugs. Accordingly, the alteration of miRNA expression is a general mechanism that plays an important pathogenic role in linking exposure to environmental toxic agents with their pathological consequences, mainly including cancer development. This review summarizes the existing experimental evidence concerning the effects of chemical carcinogens on the microRNA machinery. For each carcinogen, the specific microRNA alteration signature, as detected in experimental studies, is reported. These data are useful for applying microRNA alterations as early biomarkers of biological effects in healthy organisms exposed to environmental carcinogens. However, microRNA alteration results in carcinogenesis only if accompanied by other molecular damages. As an example, microRNAs altered by chemical carcinogens often inhibits the expression of mutated oncogenes. The long-term exposure to chemical carcinogens causes irreversible suppression of microRNA expression thus allowing the transduction into proteins of mutated oncogenes. This review also analyzes the existing knowledge regarding the mechanisms by which environmental carcinogens alter microRNA expression. The underlying molecular mechanism involves p53-microRNA interconnection, microRNA adduct formation, and alterations of Dicer function. On the whole, reported findings provide evidence that microRNA analysis is a molecular toxicology tool that can elucidate the pathogenic mechanisms activated by environmental carcinogens. Copyright © 2014 Elsevier GmbH. All rights reserved.

Whole body vibration exposure in heavy earth moving machinery operators of metalliferrous mines.

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Vanerkar, A P; Kulkarni, N P; Zade, P D; Kamavisdar, A S

2008-08-01

As mining operations get mechanized, the rate of profit generation increases and so do the rate of occupational hazards. This study deals with one such hazard - occupational vibration. The present study was carried out to determine the whole body vibration (WBV) exposure of the heavy earth moving machinery (HEMM) operators in two types of metalliferous mines in India, when they were engaged in the mining activity. Cross-comparison was done of the vibration dose value (VDV) for HEMM operators as well as each type of mine. The VDV for the shovel operator in bauxite mine was observed to be 13.53 +/- 5.63 m/s(7/4) with 25% of the readings higher than the prescribed limit whereas in iron ore mine VDV for dumper operator was 10.81 +/- 3.44 m/s(7/4) with 14.62% readings on the higher side. Cross-comparison of the VDV values for bauxite and iron ore mines revealed that it was 9.57 +/- 4.93 and 8.21 +/- 5.12 m/s(7/4) with 21.28 and 14.95% of the readings on the higher side respectively. The Student's t test level was found to be insignificant for both type of mines, indicating that the WBV exposure is not dependent on the type of mine but is dependent on the working condition and type of HEMM in operation.

Occupational exposure and health problems in small-scale industry workers in Dar es Salaam, Tanzania: a situation analysis.

NARCIS (Netherlands)

Rongo, L.M.B.; Barten, F.J.M.H.; Msamanga, G.I.; Heederik, D.; Dolmans, W.M.V.

2004-01-01

BACKGROUND: Workers in informal small-scale industries (SSI) in developing countries involved in welding, spray painting, woodwork and metalwork are exposed to various hazards with consequent risk to health. Aim To assess occupational exposure and health problems in SSI in Dar es Salaam, Tanzania.

Role of Shwachman-Bodian-Diamond syndrome protein in translation machinery and cell chemotaxis: a comparative genomics approach

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Vasieva O

2011-09-01

Full Text Available Olga VasievaInstitute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom; Fellowship for the Interpretation of Genomes, Burr Ridge, IL, USAAbstract: Shwachman-Bodian-Diamond syndrome (SBDS is linked to a mutation in a single gene. The SBDS proinvolved in RNA metabolism and ribosome-associated functions, but SBDS mutation is primarily linked to a defect in polymorphonuclear leukocytes unable to orient correctly in a spatial gradient of chemoattractants. Results of data mining and comparative genomic approaches undertaken in this study suggest that SBDS protein is also linked to tRNA metabolism and translation initiation. Analysis of crosstalk between translation machinery and cytoskeletal dynamics provides new insights into the cellular chemotactic defects caused by SBDS protein malfunction. The proposed functional interactions provide a new approach to exploit potential targets in the treatment and monitoring of this disease.Keywords: Shwachman-Bodian-Diamond syndrome, wybutosine, tRNA, chemotaxis, translation, genomics, gene proximity

A Selective Assay to Detect Chitin and Biologically Active Nano-Machineries for Chitin-Biosynthesis with Their Intrinsic Chitin-Synthase Molecules

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Hildgund Schrempf

2010-09-01

Full Text Available A new assay system for chitin has been developed. It comprises the chitin-binding protein ChbB in fusion with a His-tag as well as with a Strep-tag, the latter of which was chemically coupled to horseradish peroxidase. With the resulting complex, minimal quantities of chitin are photometrically detectable. In addition, the assay allows rapid scoring of the activity of chitin-synthases. As a result, a refined procedure for the rapid purification of yeast chitosomes (nano-machineries for chitin biosynthesis has been established. Immuno-electronmicroscopical studies of purified chitosomes, gained from a yeast strain carrying a chitin-synthase gene fused to that for GFP (green-fluorescence protein, has led to the in situ localization of chitin-synthase-GFP molecules within chitosomes.

Wavelet transform based on inner product in fault diagnosis of rotating machinery: A review

Science.gov (United States)

Chen, Jinglong; Li, Zipeng; Pan, Jun; Chen, Gaige; Zi, Yanyang; Yuan, Jing; Chen, Binqiang; He, Zhengjia

2016-03-01

As a significant role in industrial equipment, rotating machinery fault diagnosis (RMFD) always draws lots of attention for guaranteeing product quality and improving economic benefit. But non-stationary vibration signal with a large amount of noise on abnormal condition of weak fault or compound fault in many cases would lead to this task challenging. As one of the most powerful non-stationary signal processing techniques, wavelet transform (WT) has been extensively studied and widely applied in RMFD. Numerous publications about the study and applications of WT for RMFD have been presented to academic journals, technical reports and conference proceedings. Many previous publications admit that WT can be realized by means of inner product principle of signal and wavelet base. This paper verifies the essence on inner product operation of WT by simulation and field experiments. Then the development process of WT based on inner product is concluded and the applications of major developments in RMFD are also summarized. Finally, super wavelet transform as an important prospect of WT based on inner product are presented and discussed. It is expected that this paper can offer an in-depth and comprehensive references for researchers and help them with finding out further research topics.

Applications of cell-free protein synthesis in synthetic biology: Interfacing bio-machinery with synthetic environments.

Science.gov (United States)

Lee, Kyung-Ho; Kim, Dong-Myung

2013-11-01

Synthetic biology is built on the synthesis, engineering, and assembly of biological parts. Proteins are the first components considered for the construction of systems with designed biological functions because proteins carry out most of the biological functions and chemical reactions inside cells. Protein synthesis is considered to comprise the most basic levels of the hierarchical structure of synthetic biology. Cell-free protein synthesis has emerged as a powerful technology that can potentially transform the concept of bioprocesses. With the ability to harness the synthetic power of biology without many of the constraints of cell-based systems, cell-free protein synthesis enables the rapid creation of protein molecules from diverse sources of genetic information. Cell-free protein synthesis is virtually free from the intrinsic constraints of cell-based methods and offers greater flexibility in system design and manipulability of biological synthetic machinery. Among its potential applications, cell-free protein synthesis can be combined with various man-made devices for rapid functional analysis of genomic sequences. This review covers recent efforts to integrate cell-free protein synthesis with various reaction devices and analytical platforms. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Association between SNPs in microRNA-machinery genes and tuberculosis susceptibility in Chinese Tibetan population.

Science.gov (United States)

Song, Xingbo; Li, Siyue; QuCuo, MeiLang; Zhou, MeiLang; Zhou, Yi; Hu, Xin; Zhou, Juan; Lu, Xiaojun; Wang, Jun; Hua, Wei; Ye, Yuanxin; Ying, Binwu; Wang, Lanlan

2013-10-01

Tuberculosis (TB) is caused by infection with Mycobacterium tuberculosis and remains a leading cause of morbidity and mortality caused by infectious agents worldwide. Although our current understanding of the pathogenesis of TB is far from clear, there is a growing body of evidence suggesting a genetic contribution to the etiology of TB. By analyzing 294 TB cases and 287 healthy controls in a Chinese Tibetan population, we used a candidate gene approach to evaluate the association between six single nucleotide polymorphisms (rs10719, rs3757, rs3742330, rs636832, rs7813, and rs3744741) in microRNA machinery genes and TB susceptibility. The genotypic distributions of rs3757 and rs3744741 in controls were not in accordance with the Hardy–Weinberg Equilibrium (P microRNA-632 (miR-632) and that the G allele alters the affinity of microRNA-mRNA binding by disrupting the local structure of dicer 1, ribonuclease type III (DICER) mRNA, presumably allowing for upregulated DICER expression. Taken together, our data suggest that common genetic variations DICER may influence TB risk, possibly through miR-632-mediated regulation. Replication of our studies in other populations will strengthen our understanding of this association.

A Fault Diagnosis Model Based on LCD-SVD-ANN-MIV and VPMCD for Rotating Machinery

Directory of Open Access Journals (Sweden)

Songrong Luo

2016-01-01

Full Text Available The fault diagnosis process is essentially a class discrimination problem. However, traditional class discrimination methods such as SVM and ANN fail to capitalize the interactions among the feature variables. Variable predictive model-based class discrimination (VPMCD can adequately use the interactions. But the feature extraction and selection will greatly affect the accuracy and stability of VPMCD classifier. Aiming at the nonstationary characteristics of vibration signal from rotating machinery with local fault, singular value decomposition (SVD technique based local characteristic-scale decomposition (LCD was developed to extract the feature variables. Subsequently, combining artificial neural net (ANN and mean impact value (MIV, ANN-MIV as a kind of feature selection approach was proposed to select more suitable feature variables as input vector of VPMCD classifier. In the end of this paper, a novel fault diagnosis model based on LCD-SVD-ANN-MIV and VPMCD is proposed and proved by an experimental application for roller bearing fault diagnosis. The results show that the proposed method is effective and noise tolerant. And the comparative results demonstrate that the proposed method is superior to the other methods in diagnosis speed, diagnosis success rate, and diagnosis stability.

Activation of Short and Long Chain Fatty Acid Sensing Machinery in the Ileum Lowers Glucose Production in Vivo.

Science.gov (United States)

Zadeh-Tahmasebi, Melika; Duca, Frank A; Rasmussen, Brittany A; Bauer, Paige V; Côté, Clémence D; Filippi, Beatrice M; Lam, Tony K T

2016-04-15

Evidence continues to emerge detailing the myriad of ways the gut microbiota influences host energy homeostasis. Among the potential mechanisms, short chain fatty acids (SCFAs), the byproducts of microbial fermentation of dietary fibers, exhibit correlative beneficial metabolic effects in humans and rodents, including improvements in glucose homeostasis. The underlying mechanisms, however, remain elusive. We here report that one of the main bacterially produced SCFAs, propionate, activates ileal mucosal free fatty acid receptor 2 to trigger a negative feedback pathway to lower hepatic glucose production in healthy rats in vivo We further demonstrate that an ileal glucagon-like peptide-1 receptor-dependent neuronal network is necessary for ileal propionate and long chain fatty acid sensing to regulate glucose homeostasis. These findings highlight the potential to manipulate fatty acid sensing machinery in the ileum to regulate glucose homeostasis. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

A Guide for Equipping Industrial Arts Facilities.

Science.gov (United States)

American Industrial Arts Association, Washington, DC. Equipment Guide Committee.

A guide for planning new and revising existing industrial arts facilities which gives a listing of tools and equipment recommended for each of the major areas of instruction (automotive and power mechanics, ceramics, drafting, electronics, elementary, general shop, graphic arts, metalworking, plastics, and woodworking). General descriptions and…

Health education: Effect on knowledge and practice of workplace ...

African Journals Online (AJOL)

Background: There has been increasing incidence of occupational diseases among woodworkers due to exposure to preventable hazards in the workplace. The objective of this study was to determine the effects of health education on the knowledge and practice of workplace hygiene and protective measures among ...

Altered Machinery of Protein Synthesis in Alzheimer's: From the Nucleolus to the Ribosome.

Science.gov (United States)

Hernández-Ortega, Karina; Garcia-Esparcia, Paula; Gil, Laura; Lucas, José J; Ferrer, Isidre

2016-09-01

Ribosomes and protein synthesis have been reported to be altered in the cerebral cortex at advanced stages of Alzheimer's disease (AD). Modifications in the hippocampus with disease progression have not been assessed. Sixty-seven cases including middle-aged (MA) and AD stages I-VI were analyzed. Nucleolar chaperones nucleolin, nucleophosmin and nucleoplasmin 3, and upstream binding transcription factor RNA polymerase I gene (UBTF) mRNAs are abnormally regulated and their protein levels reduced in AD. Histone modifications dimethylated histone H3K9 (H3K9me2) and acetylated histone H3K12 (H3K12ac) are decreased in CA1. Nuclear tau declines in CA1 and dentate gyrus (DG), and practically disappears in neurons with neurofibrillary tangles. Subunit 28 ribosomal RNA (28S rRNA) expression is altered in CA1 and DG in AD. Several genes encoding ribosomal proteins are abnormally regulated and protein levels of translation initiation factors eIF2α, eIF3η and eIF5, and elongation factor eEF2, are altered in the CA1 region in AD. These findings show alterations in the protein synthesis machinery in AD involving the nucleolus, nucleus and ribosomes in the hippocampus in AD some of them starting at first stages (I-II) preceding neuron loss. These changes may lie behind reduced numbers of dendritic branches and reduced synapses of CA1 and DG neurons which cause hippocampal atrophy. © 2015 International Society of Neuropathology.

Repetitive transient extraction for machinery fault diagnosis using multiscale fractional order entropy infogram

Science.gov (United States)

Xu, Xuefang; Qiao, Zijian; Lei, Yaguo

2018-03-01

The presence of repetitive transients in vibration signals is a typical symptom of local faults of rotating machinery. Infogram was developed to extract the repetitive transients from vibration signals based on Shannon entropy. Unfortunately, the Shannon entropy is maximized for random processes and unable to quantify the repetitive transients buried in heavy random noise. In addition, the vibration signals always contain multiple intrinsic oscillatory modes due to interaction and coupling effects between machine components. Under this circumstance, high values of Shannon entropy appear in several frequency bands or high value of Shannon entropy doesn't appear in the optimal frequency band, and the infogram becomes difficult to interpret. Thus, it also becomes difficult to select the optimal frequency band for extracting the repetitive transients from the whole frequency bands. To solve these problems, multiscale fractional order entropy (MSFE) infogram is proposed in this paper. With the help of MSFE infogram, the complexity and nonlinear signatures of the vibration signals can be evaluated by quantifying spectral entropy over a range of scales in fractional domain. Moreover, the similarity tolerance of MSFE infogram is helpful for assessing the regularity of signals. A simulation and two experiments concerning a locomotive bearing and a wind turbine gear are used to validate the MSFE infogram. The results demonstrate that the MSFE infogram is more robust to the heavy noise than infogram and the high value is able to only appear in the optimal frequency band for the repetitive transient extraction.

Waldorf Education: An Innovative Tradition.

Science.gov (United States)

Harrington, Sheila

1993-01-01

Waldorf Schools represent the largest nonsectarian school movement in the world, shunning fads and technology and relying on the creative gifts of teachers and students. Studies include eurythmy, woodworking, weaving, and traditional academic subjects, and no commercial textbooks are used. Despite teacher/funding shortages, the system continues to…

The Presynaptic v-ATPase Reversibly Disassembles and Thereby Modulates Exocytosis but Is Not Part of the Fusion Machinery

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Anna Bodzęta

2017-08-01

Full Text Available Vacuolar H+-ATPase (v-ATPase is a multi-subunit complex comprising two domains: the cytosolic V1 domain catalyzing ATP hydrolysis and the membranous V0 sector translocating protons across membranes. In addition to proton pumping, a direct function of the V0 proteolipid ring in membrane fusion has been proposed for yeast vacuolar fusion and synaptic vesicle exocytosis in Drosophila. Here, we show in cultured hippocampal neurons that in recycling synaptic vesicles, v-ATPases are only transiently assembled in a pH-dependent fashion during the tightly coupled cycle of exo-endocytosis. Upon locking v-ATPase in an assembled state by saliphenylhalamide, we observed use- and time-dependent release depression for stimuli exceeding release of primed vesicles but no abrogation of exocytosis. Thus, the membranous V0 sector is not part of the exocytotic fusion machinery. Instead, v-ATPase modulates release upstream of docking to favor fusion of fully filled synaptic vesicles.

The V-ATPase membrane domain is a sensor of granular pH that controls the exocytotic machinery.

Science.gov (United States)

Poëa-Guyon, Sandrine; Ammar, Mohamed Raafet; Erard, Marie; Amar, Muriel; Moreau, Alexandre W; Fossier, Philippe; Gleize, Vincent; Vitale, Nicolas; Morel, Nicolas

2013-10-28

Several studies have suggested that the V0 domain of the vacuolar-type H(+)-adenosine triphosphatase (V-ATPase) is directly implicated in secretory vesicle exocytosis through a role in membrane fusion. We report in this paper that there was a rapid decrease in neurotransmitter release after acute photoinactivation of the V0 a1-I subunit in neuronal pairs. Likewise, inactivation of the V0 a1-I subunit in chromaffin cells resulted in a decreased frequency and prolonged kinetics of amperometric spikes induced by depolarization, with shortening of the fusion pore open time. Dissipation of the granular pH gradient was associated with an inhibition of exocytosis and correlated with the V1-V0 association status in secretory granules. We thus conclude that V0 serves as a sensor of intragranular pH that controls exocytosis and synaptic transmission via the reversible dissociation of V1 at acidic pH. Hence, the V-ATPase membrane domain would allow the exocytotic machinery to discriminate fully loaded and acidified vesicles from vesicles undergoing neurotransmitter reloading.

Enhanced Expression of Interferon-γ-Induced Antigen-Processing Machinery Components in a Spontaneously Occurring Cancer

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Fulvia Cerruti

2007-11-01

Full Text Available In human tumors, changes in the surface expression and/or function of major histocompatibility complex (MHC class I antigens are frequently found and may provide malignant cells with a mechanism to escape control of the immune system. This altered human lymphocyte antigen (HLA class I phenotype can be caused by either structural alterations or dysregulation of genes encoding subunits of HLA class I antigens and/or components of the MHC class I antigen-processing machinery (APM. Herein we analyze the expression of several proteins involved in the generation of MHC class I epitopes in feline injection site sarcoma, a spontaneously occurring tumor in cats that is an informative model for the study of tumor biology in other species, including humans. Eighteen surgically removed primary fibrosarcoma lesions were analyzed, and an enhanced expression of two catalytic subunits of immunoproteasomes, PA28 and leucine aminopeptidase, was found in tumors compared to matched normal tissues. As a functional counterpart of these changes in protein levels, proteasomal activities were increased in tissue extracts from fibrosarcomas. Taken together, these results suggest that alterations in the APM system may account for reduced processing of selected tumor antigens and may potentially provide neoplastic fibroblasts with a mechanism for escape from T-cell recognition and destruction.

Secondary School Design: Workshop Crafts.

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Department of Education and Science, London (England).

Design features are described for school shop facilities. Some general requirements common to most workshops are discussed; and specific design information is provided for general woodwork, general metalwork, and combined wood and metalwork facilities. The grouping of the workshop crafts and their relation to other parts of the school are also…

New insights into the interplay between the translation machinery and nonsense-mediated mRNA decay factors.

Science.gov (United States)

Raimondeau, Etienne; Bufton, Joshua C; Schaffitzel, Christiane

2018-06-19

Faulty mRNAs with a premature stop codon (PTC) are recognized and degraded by nonsense-mediated mRNA decay (NMD). Recognition of a nonsense mRNA depends on translation and on the presence of NMD-enhancing or the absence of NMD-inhibiting factors in the 3'-untranslated region. Our review summarizes our current understanding of the molecular function of the conserved NMD factors UPF3B and UPF1, and of the anti-NMD factor Poly(A)-binding protein, and their interactions with ribosomes translating PTC-containing mRNAs. Our recent discovery that UPF3B interferes with human translation termination and enhances ribosome dissociation in vitro , whereas UPF1 is inactive in these assays, suggests a re-interpretation of previous experiments and modification of prevalent NMD models. Moreover, we discuss recent work suggesting new functions of the key NMD factor UPF1 in ribosome recycling, inhibition of translation re-initiation and nascent chain ubiquitylation. These new findings suggest that the interplay of UPF proteins with the translation machinery is more intricate than previously appreciated, and that this interplay quality-controls the efficiency of termination, ribosome recycling and translation re-initiation. © 2018 The Author(s).

Proteomic Analysis of Chloroplast-to-Chromoplast Transition in Tomato Reveals Metabolic Shifts Coupled with Disrupted Thylakoid Biogenesis Machinery and Elevated Energy-Production Components1[W

Science.gov (United States)

Barsan, Cristina; Zouine, Mohamed; Maza, Elie; Bian, Wanping; Egea, Isabel; Rossignol, Michel; Bouyssie, David; Pichereaux, Carole; Purgatto, Eduardo; Bouzayen, Mondher; Latché, Alain; Pech, Jean-Claude

2012-01-01

A comparative proteomic approach was performed to identify differentially expressed proteins in plastids at three stages of tomato (Solanum lycopersicum) fruit ripening (mature-green, breaker, red). Stringent curation and processing of the data from three independent replicates identified 1,932 proteins among which 1,529 were quantified by spectral counting. The quantification procedures have been subsequently validated by immunoblot analysis of six proteins representative of distinct metabolic or regulatory pathways. Among the main features of the chloroplast-to-chromoplast transition revealed by the study, chromoplastogenesis appears to be associated with major metabolic shifts: (1) strong decrease in abundance of proteins of light reactions (photosynthesis, Calvin cycle, photorespiration) and carbohydrate metabolism (starch synthesis/degradation), mostly between breaker and red stages and (2) increase in terpenoid biosynthesis (including carotenoids) and stress-response proteins (ascorbate-glutathione cycle, abiotic stress, redox, heat shock). These metabolic shifts are preceded by the accumulation of plastid-encoded acetyl Coenzyme A carboxylase D proteins accounting for the generation of a storage matrix that will accumulate carotenoids. Of particular note is the high abundance of proteins involved in providing energy and in metabolites import. Structural differentiation of the chromoplast is characterized by a sharp and continuous decrease of thylakoid proteins whereas envelope and stroma proteins remain remarkably stable. This is coincident with the disruption of the machinery for thylakoids and photosystem biogenesis (vesicular trafficking, provision of material for thylakoid biosynthesis, photosystems assembly) and the loss of the plastid division machinery. Altogether, the data provide new insights on the chromoplast differentiation process while enriching our knowledge of the plant plastid proteome. PMID:22908117

Human Adenovirus Core Protein V Is Targeted by the Host SUMOylation Machinery To Limit Essential Viral Functions.

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Freudenberger, Nora; Meyer, Tina; Groitl, Peter; Dobner, Thomas; Schreiner, Sabrina

2018-02-15

Human adenoviruses (HAdV) are nonenveloped viruses containing a linear, double-stranded DNA genome surrounded by an icosahedral capsid. To allow proper viral replication, the genome is imported through the nuclear pore complex associated with viral core proteins. Until now, the role of these incoming virion proteins during the early phase of infection was poorly understood. The core protein V is speculated to bridge the core and the surrounding capsid. It binds the genome in a sequence-independent manner and localizes in the nucleus of infected cells, accumulating at nucleoli. Here, we show that protein V contains conserved SUMO conjugation motifs (SCMs). Mutation of these consensus motifs resulted in reduced SUMOylation of the protein; thus, protein V represents a novel target of the host SUMOylation machinery. To understand the role of protein V SUMO posttranslational modification during productive HAdV infection, we generated a replication-competent HAdV with SCM mutations within the protein V coding sequence. Phenotypic analyses revealed that these SCM mutations are beneficial for adenoviral replication. Blocking protein V SUMOylation at specific sites shifts the onset of viral DNA replication to earlier time points during infection and promotes viral gene expression. Simultaneously, the altered kinetics within the viral life cycle are accompanied by more efficient proteasomal degradation of host determinants and increased virus progeny production than that observed during wild-type infection. Taken together, our studies show that protein V SUMOylation reduces virus growth; hence, protein V SUMOylation represents an important novel aspect of the host antiviral strategy to limit virus replication and thereby points to potential intervention strategies. IMPORTANCE Many decades of research have revealed that HAdV structural proteins promote viral entry and mainly physical stability of the viral genome in the capsid. Our work over the last years showed that this

A method for estimating the contribution of seed potatoes, machinery and soil tare in field infestations with potato cyst nematodes on a national scale.

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Goeminne, M; Demeulemeester, K; Viaene, N

2011-01-01

In order to make a cost benefit analysis for the management of the potato cyst nematodes Globodera rostochiensis and G. pallida, we developed a method to estimate the relative importance of three basic distribution channels of potato cyst nematodes: seed potatoes, machinery and soil tare. The baseline is determined by the area planted with potatoes, the area infested with potato cysts, the proportion of resistant potato cultivars and the distribution of cysts trough different channels. This quantification forms a basis for the evaluation of the effects of different control measures for potato cyst nematode on a national scale. The method can be useful as an example for application in other countries.

Creativity--A Dynamic Approach to Industrial Education

Science.gov (United States)

Markowitz, John, Jr.

1974-01-01

The author presents a number of unique programs and projects which have proved successful in one high school's woodworking and graphic arts classes in terms of motivating high student interest, growth in skills, good community relations--and a financial profit. The chief objective is self-discovery, the model is Outward Bound. (AJ)

Inedible Nuts and Non-Lightning Bolts

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Rynone, William

2010-01-01

In this article, the author provides detailed information on a wide variety of commonly used screws, bolts, and other fasteners. The information has been gathered in his engineering career and outside interests (maintenance on his car and airplane, and woodworking). The topic should be of interest to students and educators in many technical…

What would be the effects of a carbon tax in Japan: an historic analysis of subsidies and fuel pricing on the iron & steel, chemical, and machinery industries

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Takako Wakiyama

2016-06-01

Full Text Available This study examines how a carbon tax could affect industrial-related carbon dioxide (CO2 emissions in Japan. Rather than forecasting the effects of a tax, the paper employs a time-series autoregressive moving average (ARMA model to determine how past subsidies and fuel price changes affected investments in energy and carbon intensity in Japan’s iron & steel, chemical, and machinery industries from 1993 to 2004. The results suggest the impacts varied greatly across industries. In the iron & steel industry, subsidies and price changes produced negligible effects on investments in energy and carbon intensity. This may be because existing iron & steel technologies have long lifetimes and substantial replacement costs. It may also be because the few large companies dominating the industry were relatively immune to subsidy provisions and pricing changes. In the chemical industry, subsidies and fuel prices gave rise to investments that improved carbon and energy intensity. This may be because the industry has relatively higher operation costs that could be cut easily given financial incentives. In the machinery industry, two of three fuel price changes (oil and gas, but not subsidy provisions, yielded improvements in carbon and energy intensity. This may reflect the heterogeneity of companies and products comprising the industry. Overall, the study underscores that policymakers need to tailor the rates and revenue recycling provisions of a carbon tax to an industry’s unique features to stimulate CO2 reductions.

A new tool for life cycle inventories of agricultural machinery operations

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Daniela Lovarelli

2016-03-01

Full Text Available The interest in environmental assessments about agricultural processes is fast growing and asking for new tools for accurate impact evaluations. The methodology commonly used to go through these studies is the life cycle assessment, of which the inventory phase (life cycle inventory, LCI is an essential step. For studies focusing on agricultural productions, the completion of LCI is particularly complex: taking into account the pedo-climatic and mechanical operative variability is evidently difficult. However, the prediction of the environmental impact of mechanical operations caused by the agricultural sector is essential to quantify the impact categories for which it is responsible. A new tool, ENVIAM, was developed to complete LCI to guarantee the availability of local data that describe the mechanical and pedo-climatic conditions occurring in the Po Valley area and widely applicable as well. It calculates mechanical power requests, directly consumed inputs (i.e., fuel, lubricant and material consumption of a productive system by taking into account soil texture, specific machinery operations and coupling solutions as defined by the user. A subdivision of working time and defined engine load have been considered to calculate fuel consumption; with regard to outputs, exhaust gases emissions from internal combustion engines have been assessed by evaluating the emissive stages of belonging as stated by the EU Directive. A case study was also performed to highlight the differences that occur when an analysis is fulfilled in a context with features different from the average, and resulted in significant variations for the inventory. In more details, a comparison was carried out both with Ecoinvent database and within ENVIAM. With regard to fuel consumption, by changing the soil texture, the analysis showed a range between 64%-184% for sandy and clay soils, respectively, if compared with medium texture ones. With this tool, local contexts defined

Language and Interactional Discourse: Deconstrusting the Talk- Generating Machinery in Natural Convresation

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Amaechi Uneke Enyi

2015-08-01

Full Text Available The study entitled. “Language and Interactional Discourse: Deconstructing the Talk - Generating Machinery in Natural Conversation,” is an analysis of spontaneous and informal conversation. The study, carried out in the theoretical and methodological tradition of Ethnomethodology, was aimed at explicating how ordinary talk is organized and produced, how people coordinate their talk –in- interaction, how meanings are determined, and the role of talk in the wider social processes. The study followed the basic assumption of conversation analysis which is, that talk is not just a product of two ‘speakers - hearers’ who attempt to exchange information or convey messages to each other. Rather, participants in conversation are seen to be mutually orienting to, and collaborating in order to achieve orderly and meaningful communication. The analytic objective is therefore to make clear these procedures on which speakers rely to produce utterances and by which they make sense of other speakers’ talk. The datum used for this study was a recorded informal conversation between two (and later three middle- class civil servants who are friends. The recording was done in such a way that the participants were not aware that they were being recorded. The recording was later transcribed in a way that we believe is faithful to the spontaneity and informality of the talk. Our finding showed that conversation has its own features and is an ordered and structured social day by- day event. Specifically, utterances are designed and informed by organized procedures, methods and resources which are tied to the contexts in which they are produced, and which participants are privy to by virtue of their membership of a culture or a natural language community.  Keywords: Language, Discourse and Conversation

Antioxidant machinery differs between melanic and light nestlings of two polymorphic raptors.

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Galván, Ismael; Gangoso, Laura; Grande, Juan M; Negro, Juan J; Rodríguez, Airam; Figuerola, Jordi; Alonso-Alvarez, Carlos

2010-10-14

Colour polymorphism results from the expression of multiallelic genes generating phenotypes with very distinctive colourations. Most colour polymorphisms are due to differences in the type or amount of melanins present in each morph, which also differ in several behavioural, morphometric and physiological attributes. Melanin-based colour morphs could also differ in the levels of glutathione (GSH), a key intracellular antioxidant, because of the role of this molecule in melanogenesis. As GSH inhibits the synthesis of eumelanin (i.e. the darkest melanin form), individuals of darker morphs are expected to have lower GSH levels than those of lighter morphs. We tested this prediction in nestlings of two polymorphic raptors, the booted eagle Hieraaetus pennatus and the Eleonora's falcon Falco eleonorae, both of which occur in two morphs differing in the extent of eumelanic plumage. As expected, melanic booted eagle nestlings had lower blood GSH levels than light morph eagle nestlings. In the Eleonora's falcon, however, melanic nestlings only had lower GSH levels after controlling for the levels of other antioxidants. We also found that melanic female eagle nestlings had higher levels of antioxidants other than GSH and were in better body condition than light female eagle nestlings. These findings suggest an adaptive response of melanic nestlings to compensate for reduced GSH levels. Nevertheless, these associations were not found in falcons, indicating species-specific particularities in antioxidant machinery. Our results are consistent with previous work revealing the importance of GSH on the expression of melanic characters that show continuous variation, and suggest that this pathway also applies to discrete colour morphs. We suggest that the need to maintain low GSH levels for eumelanogenesis in dark morph individuals may represent a physiological constraint that helps regulate the evolution and maintenance of polymorphisms.

Effects of machinery traffic in forestry on soil properties and tree growth

International Nuclear Information System (INIS)

Jansson, Karl-Johan

1998-01-01

This thesis deals with various aspects of machinery-traffic impact in forestry. A commercially available density and water content gauge was evaluated and calibrated against the traditional soil core method. Wet bulk density was measured with satisfactory accuracy with the gauge, whereas water content was not. However, this problem was solved by correcting the water content measurements using equations developed as part of the study. The gauge was thus found to be a useful tool for determining dry bulk density. The two principle ground-contact organs of a vehicle, i.e. wheels and tracks, were compared in terms of their influence on some soil physical properties after various numbers of passes. The depth to which the soil was compacted was about 40-50 cm for both vehicles and they altered measured soil properties to similar extents except in the uppermost 5-10 cm. The increase in bulk density in the topsoil caused by the wheeled vehicle was not as great as that caused by the tracked one. The effects of traffic on tree growth after mechanized cleaning operations were studied at various sites in southern and middle Sweden. The machines used were light and only shallow ruts were formed. Neither of the machines increased penetration resistance to any greater extent. Three years after the trafficking treatment, no statistically significant differences in growth increment were found between trees standing close to a rut and trees at a larger distance from the rut. Finally, the effect of static load on fine roots of Norway spruce and Scots pine were assessed in the laboratory. Pine, but not spruce, showed a reduced root growth capacity after roots had been exposed to load, while static load reduced the ability of spruce roots to reduce TTC but had no corresponding effect on pine roots. No differences were found in electrolyte leakage 74 refs, 5 figs

STUDY REGARDING THE OPERATING SPEED OF FORAGE HARVESTING MACHINERY IN ORDER TO OPTIMIZE THE WORKING CAPACITY AND FUEL CONSUME

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V. MOISE

2007-10-01

Full Text Available The efficiency of forage production machinery system is partially reflected in someclassical economical indices, because the real efficiency of mechanical tillageutilization at forage harvesting and preparation must be reflected in the foragequality increasing and feed superior valorization by the farm livestock. An area of7.8 alfalfa ha was harvested with U-650M tractor and ROTO 165 rotary mower andwith U-650M tractor and Zakaz rotary mower at different speeds in field work.Average values of the speed in parcel work, work effective capacity, work capacityduring shift, specific work capacity on cutter width, fuel consume per land unit, andspecific fuel consume per mass unit were determined for each speed in field work.Power matching between the tractor and the mower do not always meet therequirements when forming harvesting mechanized systems. Classical mowers withcommon cutting units do not sufficiently load the U-650M tractor existing inmany Romanian farms, which determines an unfavorable functioning regime ofthe tractor engine correlated with increased fuel consumes.

A study on the detection of misfiring cylinder of the diesel engine in excavation machinery using improved ALM analysis

Energy Technology Data Exchange (ETDEWEB)

Nagayama, M.; Kawamura, Y. [Tsukuba Univ., Tsukuba, Ibaraki (Japan); Ujihira, M. [Hokkaido Univ., Sapporo (Japan). School of Engineering; Akhmetov, D.F.; Ito, F.; Aoshima, N. [NTT DoCoMo Inc. (Japan)

2005-07-01

Mechanical problems in large-scale mining equipment can result in serious losses in operational efficiency and profits. A misfiring cylinder in a diesel engine can cause engine failure in a short period of time. Regular maintenance and inspection of machinery is therefore critical for mining and construction operations. For that reason, an innovative method was developed to detect whether a cylinder is misfiring, and if so, which one. This study established a simple, reliable and sure method using the improved aggregative learning method (ALM) analysis for the early diagnosis and repair of misfiring cylinders. A minimum number of sensors were applied on the side of 2 cylinders on a diesel engine in order to set up an abnormal diagnostic system that detects anomalies at an early stage by analyzing the acceleration waveform using the ALM method. The detection method is better than traditional methods that require accelerometers. 6 refs., 1 tab., 5 figs.

Genomic, proteomic and biochemical analysis of the chitinolytic machinery of Serratia marcescens BJL200.

Science.gov (United States)

Tuveng, Tina R; Hagen, Live Heldal; Mekasha, Sophanit; Frank, Jeremy; Arntzen, Magnus Øverlie; Vaaje-Kolstad, Gustav; Eijsink, Vincent G H

2017-04-01

The chitinolytic machinery of Serratia marcescens BJL200 has been studied in detail over the last couple of decades, however, the proteome secreted by this Gram-negative bacterium during growth on chitin has not been studied in depth. In addition, the genome of this most studied chitinolytic Serratia strain has until now, not been sequenced. We report a draft genome sequence for S. marcescens BJL200. Using label-free quantification (LFQ) proteomics and a recently developed plate-method for assessing secretomes during growth on solid substrates, we find that, as expected, the chitin-active enzymes (ChiA, B, C, and CBP21) are produced in high amounts when the bacterium grows on chitin. Other proteins produced in high amounts after bacterial growth on chitin provide interesting targets for further exploration of the proteins involved in degradation of chitin-rich biomasses. The genome encodes a fourth chitinase (ChiD), which is produced in low amounts during growth on chitin. Studies of chitin degradation with mixtures of recombinantly produced chitin-degrading enzymes showed that ChiD does not contribute to the overall efficiency of the process. ChiD is capable of converting N,N'-diacetyl chitobiose to N-acetyl glucosamine, but is less efficient than another enzyme produced for this purpose, the Chitobiase. Thus, the role of ChiD in chitin degradation, if any, remains unclear. Copyright © 2017 Elsevier B.V. All rights reserved.

Theoretical research for natural circulation operational characteristic of ship nuclear machinery under ocean conditions

Energy Technology Data Exchange (ETDEWEB)

Yan Binghuo [Department of Nuclear Science and Engineering, Naval University of Engineering, Wuhan 430033 (China)], E-mail: yanbh1986@163.com; Yu Lei [Department of Nuclear Science and Engineering, Naval University of Engineering, Wuhan 430033 (China)], E-mail: yulei301@163.com

2009-06-15

Based on the two-phase drift flux model and the multi-pressure nodes matrix solving method, natural circulation thermal hydraulic analysis models for the Nuclear Machinery (NM) under ocean conditions are developed. The neutron physical activities and the responses of the reactivity control systems are described by the two-group, 3-dimensional space and time dependent neutron kinetics model. Reactivity feedback is calculated by coupling the neutron physics and thermal hydraulic codes, and is tested by comparison with experiments. Using the models developed, the natural circulation operating characteristics of NM in rolling and pitching motions and the transitions between forced circulation (FC) to natural circulation (NC) are analyzed. The results show that the influence of the rolling motion increases as the rolling amplitude is increased, and as the rolling period becomes shorter. The results also show that for this NM, with the same rolling period and rolling angle, the influence of pitching motion on natural circulation is greater than that of rolling motion. Furthermore, the oscillation period for pitching motion is the same as the pitching period, while the oscillation period for rolling is one half of the rolling period. In the ocean environment, excessive flow oscillation of the natural circulation may cause the control rods to respond so frequently that the NM would not be able to realize the transition from the FC to NC steadily. However, the influence of ocean environment on the transition from NC to FC is limited.

An SVM-Based Classifier for Estimating the State of Various Rotating Components in Agro-Industrial Machinery with a Vibration Signal Acquired from a Single Point on the Machine Chassis

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Ruben Ruiz-Gonzalez

2014-11-01

Full Text Available The goal of this article is to assess the feasibility of estimating the state of various rotating components in agro-industrial machinery by employing just one vibration signal acquired from a single point on the machine chassis. To do so, a Support Vector Machine (SVM-based system is employed. Experimental tests evaluated this system by acquiring vibration data from a single point of an agricultural harvester, while varying several of its working conditions. The whole process included two major steps. Initially, the vibration data were preprocessed through twelve feature extraction algorithms, after which the Exhaustive Search method selected the most suitable features. Secondly, the SVM-based system accuracy was evaluated by using Leave-One-Out cross-validation, with the selected features as the input data. The results of this study provide evidence that (i accurate estimation of the status of various rotating components in agro-industrial machinery is possible by processing the vibration signal acquired from a single point on the machine structure; (ii the vibration signal can be acquired with a uniaxial accelerometer, the orientation of which does not significantly affect the classification accuracy; and, (iii when using an SVM classifier, an 85% mean cross-validation accuracy can be reached, which only requires a maximum of seven features as its input, and no significant improvements are noted between the use of either nonlinear or linear kernels.

Rapid RNase L-driven arrest of protein synthesis in the dsRNA response without degradation of translation machinery.

Science.gov (United States)

Donovan, Jesse; Rath, Sneha; Kolet-Mandrikov, David; Korennykh, Alexei

2017-11-01

Mammalian cells respond to double-stranded RNA (dsRNA) by activating a translation-inhibiting endoribonuclease, RNase L. Consensus in the field indicates that RNase L arrests protein synthesis by degrading ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs). However, here we provide evidence for a different and far more efficient mechanism. By sequencing abundant RNA fragments generated by RNase L in human cells, we identify site-specific cleavage of two groups of noncoding RNAs: Y-RNAs, whose function is poorly understood, and cytosolic tRNAs, which are essential for translation. Quantitative analysis of human RNA cleavage versus nascent protein synthesis in lung carcinoma cells shows that RNase L stops global translation when tRNAs, as well as rRNAs and mRNAs, are still intact. Therefore, RNase L does not have to degrade the translation machinery to stop protein synthesis. Our data point to a rapid mechanism that transforms a subtle RNA cleavage into a cell-wide translation arrest. © 2017 Donovan et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

Experiences with fuels B30 and B 100 in haulage, railway operation and agricultural machinery

Energy Technology Data Exchange (ETDEWEB)

Matejovsky, V. [QMS Consulting, Prague (Czech Republic); Hendrych, K.; Mares, V. [PREOL, Lovosice (Czech Republic)

2013-06-01

High prices of diesel fuel have increased an interest in cheaper biodiesel, especially for vehicles with high fuel consumption and not only for haulage vehicle parks but also for railway vehicles and heavy agricultural machinery. When price difference between standard diesel B7 and cheaper biodiesel B100 reached more than 10% it was a sufficient benefit for operators to use biodiesel but this fuel had not been approved for all vehicles types by their manufacturers. Despite this problem, some operators have begun to use biodiesel also for vehicles not having the approval. To prevent operational problems and misgiving of engines damage, the transition to alternative fuel was organized as field tests of one or more vehicles from the operator's fleet. The tests usually started with B30 fuel and if no operational problems occurred the second stage continued with B100. The tested vehicles were under permanent surveillance at least during one year of operation and once a month and later once in a quarter a deeper inspections were made including engine diagnostics, emissions testing, engine oil sampling for laboratory examination, injectors tenting and filters and fuel hoses condition evaluation. The presentation includes the results of vehicles inspections and the measures that had to be done to prevent engines failure and to ensure trouble-free operation of vehicles using biofuels. (orig.)

Effect of ploughing-down of grapevine chips on soil structure when using special agricultural machinery

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Barbora Badalíková

2012-01-01

Full Text Available Within the period of 2008–2011, changes in soil structure were studied in two selected localities: one of them was situated in vineyards of the University Training Farm of Mendel University in Žabčice near Brno, the other was in vineyards situated in the cadastre of wine-growing municipality Velké Bílovice. Established were altogether three variants of experiments with application of crushed grapevine wood (chips: Variant 1 – control; Variant 2 – crushed grapevine wood ploughed down to the depth of 0.10 m; Variant 3 – crushed grapevine wood + grass spread on the soil surface as a mulch. Grapevine canes were crushed to chips using a special agricultural machinery while the soil in inter-rows was processed using conventional tilling machines. The obtained results showed that the best coefficient of structurality (expressing the degree of destruction of soil structure was recorded in Variants 2 in both localities. Considering values of this coefficient it could be concluded that just this variant showed a positive effect on soil structure. This variant reduced the compaction of soil caused by the movement of agricultural machines in vineyard inter-rows Crushed grapevine waste wood can therefore compensate losses of organic matter in soil. Better values of structurality coefficient were recorded in the locality Žabčice.

Adaptive Wavelet Threshold Denoising Method for Machinery Sound Based on Improved Fruit Fly Optimization Algorithm

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Jing Xu

2016-07-01

Full Text Available As the sound signal of a machine contains abundant information and is easy to measure, acoustic-based monitoring or diagnosis systems exhibit obvious superiority, especially in some extreme conditions. However, the sound directly collected from industrial field is always polluted. In order to eliminate noise components from machinery sound, a wavelet threshold denoising method optimized by an improved fruit fly optimization algorithm (WTD-IFOA is proposed in this paper. The sound is firstly decomposed by wavelet transform (WT to obtain coefficients of each level. As the wavelet threshold functions proposed by Donoho were discontinuous, many modified functions with continuous first and second order derivative were presented to realize adaptively denoising. However, the function-based denoising process is time-consuming and it is difficult to find optimal thresholds. To overcome these problems, fruit fly optimization algorithm (FOA was introduced to the process. Moreover, to avoid falling into local extremes, an improved fly distance range obeying normal distribution was proposed on the basis of original FOA. Then, sound signal of a motor was recorded in a soundproof laboratory, and Gauss white noise was added into the signal. The simulation results illustrated the effectiveness and superiority of the proposed approach by a comprehensive comparison among five typical methods. Finally, an industrial application on a shearer in coal mining working face was performed to demonstrate the practical effect.

An improvement of agricultural equipment manufacturing by using lean techniques : A case study of agricultural machinery company

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Nattariya Pakdeepunya

2015-06-01

Full Text Available The objective of this research was to study and improve the manufacturing process of agricultural equipment to reduce wastes by using the LEAN tools: a case study of agricultural machinery company. This study found that the main wastes were inefficiency of transportation and working process. The Systematic Layout Planning Pattern (SLP had been applied. Moreover, the principles of ECRS, the change in input factors (a number of employees, together with simulation and the Analysis Hierarchy Process (AHP were also used to determine the appropriate plant layout alternatives by considering the factors such as the productivity, materials handling distance, facilities utilization, and space utilization. The result shared that the best suitable layout is the layout No. 1. Then more improvement the layout No. 1 with the ECRS and the change of input factors (the amount of employees ,it was found that increased productivity by 16.10%, increased facilities utilization by 15.73% and decreased materials handling distance by 48.12%. Furthermore, the number of employees and the average time of workpiece in system could be reduced from 7 to 6 persons, and 54.41 minutes to 45.65 minutes respectively.

Dynamic expression of the translational machinery during Bacillus subtilis life cycle at a single cell level.

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Alex Rosenberg

Full Text Available The ability of bacteria to responsively regulate the expression of translation components is crucial for rapid adaptation to fluctuating environments. Utilizing Bacillus subtilis (B. subtilis as a model organism, we followed the dynamics of the translational machinery at a single cell resolution during growth and differentiation. By comprehensive monitoring the activity of the major rrn promoters and ribosomal protein production, we revealed diverse dynamics between cells grown in rich and poor medium, with the most prominent dissimilarities exhibited during deep stationary phase. Further, the variability pattern of translational activity varied among the cells, being affected by nutrient availability. We have monitored for the first time translational dynamics during the developmental process of sporulation within the two distinct cellular compartments of forespore and mother-cell. Our study uncovers a transient forespore specific increase in expression of translational components. Finally, the contribution of each rrn promoter throughout the bacterium life cycle was found to be relatively constant, implying that differential expression is not the main purpose for the existence of multiple rrn genes. Instead, we propose that coordination of the rrn operons serves as a strategy to rapidly fine tune translational activities in a synchronized fashion to achieve an optimal translation level for a given condition.

Protein Machineries Involved in the Attachment of Heme to Cytochrome c: Protein Structures and Molecular Mechanisms

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Carlo Travaglini-Allocatelli

2013-01-01

Full Text Available Cytochromes c (Cyt c are ubiquitous heme-containing proteins, mainly involved in electron transfer processes, whose structure and functions have been and still are intensely studied. Surprisingly, our understanding of the molecular mechanism whereby the heme group is covalently attached to the apoprotein (apoCyt in the cell is still largely unknown. This posttranslational process, known as Cyt c biogenesis or Cyt c maturation, ensures the stereospecific formation of the thioether bonds between the heme vinyl groups and the cysteine thiols of the apoCyt heme binding motif. To accomplish this task, prokaryotic and eukaryotic cells have evolved distinctive protein machineries composed of different proteins. In this review, the structural and functional properties of the main maturation apparatuses found in gram-negative and gram-positive bacteria and in the mitochondria of eukaryotic cells will be presented, dissecting the Cyt c maturation process into three functional steps: (i heme translocation and delivery, (ii apoCyt thioreductive pathway, and (iii apoCyt chaperoning and heme ligation. Moreover, current hypotheses and open questions about the molecular mechanisms of each of the three steps will be discussed, with special attention to System I, the maturation apparatus found in gram-negative bacteria.

Fault Diagnosis of Rotating Machinery Based on the Multiscale Local Projection Method and Diagonal Slice Spectrum

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Yong Lv

2018-04-01

Full Text Available The vibration signals of bearings and gears measured from rotating machinery usually have nonlinear, nonstationary characteristics. The local projection algorithm cannot only reduce the noise of the nonlinear system, but can also preserve the nonlinear deterministic structure of the signal. The influence of centroid selection on the performance of noise reduction methods is analyzed, and the multiscale local projection method of centroid was proposed in this paper. This method considers both the geometrical shape and statistical error of the signal in high dimensional phase space, which can effectively eliminate the noise and preserve the complete geometric structure of the attractors. The diagonal slice spectrum can identify the frequency components of quadratic phase coupling and enlarge the coupled frequency component in the nonlinear signal. Therefore, the proposed method based on the above two algorithms can achieve more accurate results of fault diagnosis of gears and rolling bearings. The simulated signal is used to verify its effectiveness in a numerical simulation. Then, the proposed method is conducted for fault diagnosis of gears and rolling bearings in application researches. The fault characteristics of faulty bearings and gears can be extracted successfully in the researches. The experimental results indicate the effectiveness of the novel proposed method.

Enhancing the safety and efficiency of the driving gear of coal mining machinery by using water as a hydraulic fluid and enhancing the reliability of scraper-chain conveyors; Erhoehung der Sicherheit und Leistungsfaehigkeit der Antriebstechnik von Arbeitsmaschinen durch Verwendung von Wasserhydraulik sowie Erhoehung der Zuverlaessigkeit der Kettenkratzerfoerderer

Energy Technology Data Exchange (ETDEWEB)

Reichel, J.; Boeing, R.; Graetz, A.; Loehning, H.D.; Plum, D.

1997-12-31

The objective pursued is to increasingly use water or high water-content fluids as a substitute for other hydraulic fluids in driving gear of mining machinery. The state of the art of the technology is represented only by individual solutions achieved for given purposes which are not suitable for other applications, let alone for coal mining machinery. The research project was to identify hydraulic components that will permit the use of water or watery substances as a hydraulic fluid in mining applications. The components have been found and further developed, and finally systems with linear and rotatory drives have been tested at various test facilities in order to derive information on the system behaviour of pressurized fluids and machinery components and their suitability for coal mining applications. (orig./CB) [Deutsch] In der untertaegigen Antriebstechnik sollen vermehrt Wasser und wasserhaltige Fluessigkeiten eingesetzt werden. Der Stand der Technik fuehrt bei der Anwendung von Wasserhydraulik immer wieder nur Einzelloesungen auf, die nicht allgemein und insbesondere im Steinkohlenbergbau angewendet werden koennen. Im Rahmen dieses Forschungsvorhabens wurden fuer die Wasserhydraulik geeignete Komponenten untersucht, weiterentwickelt und schliesslich Systeme mit linearen und rotatorischen Antrieben auf verschiedenen Pruefstaenden erprobt, um Aussagen ueber das Systemverhalten von Druckfluessigkeit und Bauelementen fuer Bergbauanwendungen zu bekommen. (orig./MSK)

我国大豆精量播种机械发展现状及趋势%Development Status and Trends of Soybean Precision Seeding Machinery in China

Institute of Scientific and Technical Information of China (English)

张晓刚; 刘伟; 余永昌

2012-01-01

　　大豆播种机械作为大豆机械化生产中的主要工具，其性能的优劣直接影响到播种质量及大豆产量。介绍了我国东北地区、黄淮海区域和南方区域大豆精密播种技术的应用现状，阐述了大豆精量排种器以及免耕防堵技术的理论研究现状，指出我国大豆精密播种技术需要解决的关键问题，从而为我国大豆精量播种机械的发展方向提供参考。%　　As the main tool of soybean mechanization production, the quality performance of the soybean planting machinery, directly influences the sowing quality and soybean yield. This article reviewed the present situation of soybean precision seeding technology application in Northeastern, Huang-huai-hai and the Southern regions, and elaborated the theoretical status on soybean precise metering device and no-tillage block-up defense technology in China. Key problems in soybean precision planting technology were pointed out, which provided a reference for the development of soybean precision sowing machinery in China.

The Art and Craft of Science

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Root-Bernstein, Robert; Root-Bernstein, Michele

2013-01-01

Walter Alvarez, a doctor and physiologist of some renown, decided to send his scientifically talented son, Luis, to an arts and crafts school where Luis took industrial drawing and woodworking instead of calculus. Luis Alvarez won the Nobel Prize in physics in 1968. Einstein was certainly not a standout in his mathematics and physics classes. Yet…

How small firms contrast with large firms regarding perceptions, practices, and needs in the U.S

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Urs Buehlmann; Matthew Bumgardner; Michael. Sperber

2013-01-01

As many larger secondary woodworking firms have moved production offshore and been adversely impacted by the recent housing downturn, smaller firms have become important to driving U.S. hardwood demand. This study compared and contrasted small and large firms on a number of factors to help determine the unique characteristics of small firms and to provide insights into...

Recent advances in forest products research and development

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Joseph E. Jakes

2016-01-01

Wood has always played an integral role in meeting our materials needs. The earliest evidence of woodworking dates back 1.5 million years to the modern human ancestor Homo erectus.1 Many characteristics that made wood a favorable material for our ancestors—relative abundance, ease of forming and shaping, exceptional strength-toweight ratio,...

Housing's impact on wood products sales

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Matt Bumgardner; Urs Buehlmann; Karen. Koenig

2017-01-01

What are the market conditions for woodworkers involved in construction-based sectors and what investments are planned to improve capabilities in the current business environment? For a fifth straight year, construction-based mar­kets continued their growth trends in 2016, as U.S. spending on single family housing, multi-family housing, and nonresidential construction...

Tools. Unit 9: A Core Curriculum of Related Instruction for Apprentices.

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New York State Education Dept., Albany. Bureau of Occupational and Career Curriculum Development.

The tool handling unit is presented to assist apprentices to acquire a general knowledge on the use of various basic tools. The unit consists of seven modules: (1) introduction to hand tools and small power tools; (2) measuring tools: layout and measuring tools for woodworking; (3) measuring tools: feeler gauge, micrometer, and torque wrench; (4)…

Housing and the wood industry, trends & market conditions

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Urs Buehlmann; Matt Bumgardner; Al Schuler; K. Koenig

2011-01-01

Housing markets continue to have major impacts on the secondary wood industry. So, what are the steps being taken by wood products manufacturers in order to stay viable? As a follow-up to last year's article, "Housing Market's Impact on the Secondary Woodworking Industry" (Wood & Wood Products, July 2010), the focus of this year's study was...

Output-only cyclo-stationary linear-parameter time-varying stochastic subspace identification method for rotating machinery and spinning structures

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Velazquez, Antonio; Swartz, R. Andrew

2015-02-01

Economical maintenance and operation are critical issues for rotating machinery and spinning structures containing blade elements, especially large slender dynamic beams (e.g., wind turbines). Structural health monitoring systems represent promising instruments to assure reliability and good performance from the dynamics of the mechanical systems. However, such devices have not been completely perfected for spinning structures. These sensing technologies are typically informed by both mechanistic models coupled with data-driven identification techniques in the time and/or frequency domain. Frequency response functions are popular but are difficult to realize autonomously for structures of higher order, especially when overlapping frequency content is present. Instead, time-domain techniques have shown to possess powerful advantages from a practical point of view (i.e. low-order computational effort suitable for real-time or embedded algorithms) and also are more suitable to differentiate closely-related modes. Customarily, time-varying effects are often neglected or dismissed to simplify this analysis, but such cannot be the case for sinusoidally loaded structures containing spinning multi-bodies. A more complex scenario is constituted when dealing with both periodic mechanisms responsible for the vibration shaft of the rotor-blade system and the interaction of the supporting substructure. Transformations of the cyclic effects on the vibrational data can be applied to isolate inertial quantities that are different from rotation-generated forces that are typically non-stationary in nature. After applying these transformations, structural identification can be carried out by stationary techniques via data-correlated eigensystem realizations. In this paper, an exploration of a periodic stationary or cyclo-stationary subspace identification technique is presented here for spinning multi-blade systems by means of a modified Eigensystem Realization Algorithm (ERA) via

Falls From Agricultural Machinery: Risk Factors Related to Work Experience, Worked Hours, and Operators' Behavior.

Science.gov (United States)

Caffaro, Federica; Roccato, Michele; Micheletti Cremasco, Margherita; Cavallo, Eugenio

2018-02-01

Objective We investigated the risk factors for falls when egressing from agricultural tractors, analyzing the role played by worked hours, work experience, operators' behavior, and near misses. Background Many accidents occur within the agricultural sector each year. Among them, falls while dismounting the tractor represent a major source of injuries. Previous studies pointed out frequent hazardous movements and incorrect behaviors adopted by operators to exit the tractor cab. However, less is known about the determinants of such behaviors. In addition, near misses are known to be important predictors of accidents, but they have been under-investigated in the agricultural sector in general and as concerns falls in particular. Method A questionnaire assessing dismounting behaviors, previous accidents and near misses, and participants' relation with work was administered to a sample of Italian tractor operators ( n = 286). Results A mediated model showed that worked hours increase unsafe behaviors, whereas work experience decreases them. Unsafe behaviors in turn show a positive association with accidents, via the mediation of near misses. Conclusions We gave a novel contribution to the knowledge of the chain of events leading to fall accidents in the agricultural sector, which is one of the most hazardous industries. Applications Besides tractor design improvements, preventive training interventions may focus on the redesign of the actual working strategies and the adoption of engaging training methods in the use of machinery to optimize the learning of safety practices and safe behaviors.