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Sample records for lysis processes estudio

  1. Study of the phage production efficiency in the bacteria lysis processes; Estudio del rendimiento en fagos para los procesos de lisis bacteriana

    Energy Technology Data Exchange (ETDEWEB)

    Vidania Munoz, R de; Garces, F; Davila, C A

    1979-07-01

    In this work we present a search for the best production conditions of {lambda}vir and{lambda} clear phages In E coli K12 and E coli C{sub 6}00 infected cells respectively. By keeping fixed some parameters of the process as the bacterial and phage generation times and the bacterial burst side, we have found that the lysis yield is strongly dependent on the multiplicity and in a lesser degree on the infection time. It appears from the experimental results that other variables are important, as infection efficiency and approach time from phages to bacteria. We will try to describe the lysis phenomenon by a numerical model on the bases of the se experimental results. (Author) 11 refs.

  2. Study of the phage production efficiency in the bacteria lysis processes

    International Nuclear Information System (INIS)

    Vidania Munoz, R. de; Garces, F.; Davila, C. A.

    1979-01-01

    In this work we present a search for the best production conditions of λvir andλ clear phages In E coli K12 and E coli C 6 00 infected cells respectively. By keeping fixed some parameters of the process as the bacterial and phage generation times and (he bacterial burst side, we have finder that the lysis yield is strongly dependent on the multiplicity and in a lesser degree on the infection time. It appears from the experimental results that other variables are important, as infection efficiency and approach time from phages to bacteria. We will try to describe the lysis phenomenon by a numerical model on the bases of the se experimental results. (Author) 11 refs

  3. Artificial intelligence versus statistical modeling and optimization of continuous bead milling process for bacterial cell lysis

    Directory of Open Access Journals (Sweden)

    Shafiul Haque

    2016-11-01

    Full Text Available AbstractFor a commercially viable recombinant intracellular protein production process, efficient cell lysis and protein release is a major bottleneck. The recovery of recombinant protein, cholesterol oxidase (COD was studied in a continuous bead milling process. A full factorial Response Surface Model (RSM design was employed and compared to Artificial Neural Networks coupled with Genetic Algorithm (ANN-GA. Significant process variables, cell slurry feed rate (A, bead load (B, cell load (C and run time (D, were investigated and optimized for maximizing COD recovery. RSM predicted an optimum of feed rate of 310.73 mL/h, bead loading of 79.9% (v/v, cell loading OD600 nm of 74, and run time of 29.9 min with a recovery of ~3.2 g/L. ANN coupled with GA predicted a maximum COD recovery of ~3.5 g/L at an optimum feed rate (mL/h: 258.08, bead loading (%, v/v: 80%, cell loading (OD600 nm: 73.99, and run time of 32 min. An overall 3.7-fold increase in productivity is obtained when compared to a batch process. Optimization and comparison of statistical vs. artificial intelligence techniques in continuous bead milling process has been attempted for the very first time in our study. We were able to successfully represent the complex non-linear multivariable dependence of enzyme recovery on bead milling parameters. The quadratic second order response functions are not flexible enough to represent such complex non-linear dependence. ANN being a summation function of multiple layers are capable to represent complex non-linear dependence of variables in this case; enzyme recovery as a function of bead milling parameters. Since GA can even optimize discontinuous functions present study cites a perfect example of using machine learning (ANN in combination with evolutionary optimization (GA for representing undefined biological functions which is the case for common industrial processes involving biological moieties.

  4. Factors influencing lysis time stochasticity in bacteriophage λ

    Directory of Open Access Journals (Sweden)

    Dennehy John J

    2011-08-01

    Full Text Available Abstract Background Despite identical genotypes and seemingly uniform environments, stochastic gene expression and other dynamic intracellular processes can produce considerable phenotypic diversity within clonal microbes. One trait that provides a good model to explore the molecular basis of stochastic variation is the timing of host lysis by bacteriophage (phage. Results Individual lysis events of thermally-inducible λ lysogens were observed using a temperature-controlled perfusion chamber mounted on an inverted microscope. Both mean lysis time (MLT and its associated standard deviation (SD were estimated. Using the SD as a measure of lysis time stochasticity, we showed that lysogenic cells in controlled environments varied widely in lysis times, and that the level of lysis time stochasticity depended on allelic variation in the holin sequence, late promoter (pR' activity, and host growth rate. In general, the MLT was positively correlated with the SD. Both lower pR' activities and lower host growth rates resulted in larger SDs. Results from premature lysis, induced by adding KCN at different time points after lysogen induction, showed a negative correlation between the timing of KCN addition and lysis time stochasticity. Conclusions Taken together with results published by others, we conclude that a large fraction of λ lysis time stochasticity is the result of random events following the expression and diffusion of the holin protein. Consequently, factors influencing the timing of reaching critical holin concentrations in the cell membrane, such as holin production rate, strongly influence the mean lysis time and the lysis time stochasticity.

  5. Maintenance, endogeneous, respiration, lysis, decay and predation

    DEFF Research Database (Denmark)

    loosdrecht, Marc C. M. Van; Henze, Mogens

    1999-01-01

    mechanism is microbiologically correct. The lysis/decay model mechanism is a strongly simplified representation of reality. This paper tries to review the processes grouped under endogenous respiration in activated sludge models. Mechanisms and processes such as maintenance, lysis, internal and external...... decay, predation and death-regeneration are discussed. From recent microbial research it has become evident that cells do not die by themselves. Bacteria are however subject to predation by protozoa. Bacteria store reserve polymers that in absence of external substrate are used for growth...

  6. Lysis from without

    Science.gov (United States)

    2011-01-01

    In this commentary I consider use of the term “lysis from without” (LO) along with the phenomenon's biological relevance. LO originally described an early bacterial lysis induced by high-multiplicity virion adsorption and that occurs without phage production (here indicated as LOV). Notably, this is more than just high phage multiplicities of adsorption leading to bacterial killing. The action on bacteria of exogenously supplied phage lysin, too, has been described as a form of LO (here, LOL). LOV has been somewhat worked out mechanistically for T4 phages, has been used to elucidate various phage-associated phenomena including discovery of the phage eclipse, may be relevant to phage ecology, and, with resistance to LO (LOR), is blocked by certain phage gene products. Speculation as to the impact of LOV on phage therapy also is fairly common. Since LOV assays are relatively easily performed and not all phages are able to induce LOV, a phage's potential to lyse bacteria without first infecting should be subject to at least in vitro experimental confirmation before the LOV label is applied. The term “abortive infection” may be used more generally to describe non-productive phage infections that kill bacteria. PMID:21687534

  7. Spontaneous Tumor Lysis Syndrome

    Directory of Open Access Journals (Sweden)

    Alicia C. Weeks MD

    2015-08-01

    Full Text Available Tumor lysis syndrome (TLS is a known complication of malignancy and its treatment. The incidence varies on malignancy type, but is most common with hematologic neoplasms during cytotoxic treatment. Spontaneous TLS is thought to be rare. This case study is of a 62-year-old female admitted with multisystem organ failure, with subsequent diagnosis of aggressive B cell lymphoma. On admission, laboratory abnormalities included renal failure, elevated uric acid (20.7 mg/dL, and 3+ amorphous urates on urinalysis. Oliguric renal failure persisted despite aggressive hydration and diuretic use, requiring initiation of hemodialysis prior to chemotherapy. Antihyperuricemic therapy and hemodialysis were used to resolve hyperuricemia. However, due to multisystem organ dysfunction syndrome with extremely poor prognosis, the patient ultimately expired in the setting of a terminal ventilator wean. Although our patient did not meet current TLS criteria, she required hemodialysis due to uric acid nephropathy, a complication of TLS. This poses the clinical question of whether adequate diagnostic criteria exist for spontaneous TLS and if the lack of currently accepted guidelines has resulted in the underestimation of its incidence. Allopurinol and rasburicase are commonly used for prevention and treatment of TLS. Although both drugs decrease uric acid levels, allopurinol mechanistically prevents formation of the substrate rasburicase acts to solubilize. These drugs were administered together in our patient, although no established guidelines recommend combined use. This raises the clinical question of whether combined therapy is truly beneficial or, conversely, detrimental to patient outcomes.

  8. Tumor lysis syndrome in children

    International Nuclear Information System (INIS)

    Suarez, Amaranto

    2004-01-01

    Tumor lysis syndrome is a metabolic emergency characterized by electrolyte alteration with or without acute renal failure. It occurs mainly in patients with malignant tumors that have a high growth fraction, or after cytotoxic therapy, as a result of the massive degradation of malignant cells and the release of high amounts of intracellular elements that exceed the capacity of renal excretion. The objective of the treatment is the prevention of nephropathy due to uric acid deposits, and the correction of metabolic acidosis and electrolyte alterations. This paper reviews the incidence, the physiopathology, and the treatment of tumor lysis syndrome in children

  9. Synthesis and functioning of the colicin E1 lysis protein: Comparison with the colicin A lysis protein

    International Nuclear Information System (INIS)

    Cavard, D.

    1991-01-01

    The colicin E1 lysis protein, CelA, was identified as a 3-kDa protein in induced cells of Escherichia coli K-12 carrying pColE1 by pulse-chase labeling with either [ 35 S]cysteine or [ 3 H]lysine. This 3-kDa protein was acylated, as shown by [2- 3 H]glycerol labeling, and seemed to correspond to the mature CelA protein. The rate of modification and processing of CelA was different from that observed for Cal, the colicin A lysis protein. In contrast to Cal, no intermediate form was detected for CelA, no signal peptide accumulated, and no modified precursor form was observed after globomycin treatment. Thus, the rate of synthesis would not be specific to lysis proteins. Solubilization in sodium dodecyl sulfate of the mature forms of both CelA and Cal varied similarly at the time of colicin release, indicating a change in lysis protein structure. This particular property would play a role in the mechanism of colicin export. The accumulation of the signal peptide seems to be a factor determining the toxicity of the lysis proteins since CelA provoked less cell damage than Cal. Quasi-lysis and killing due to CelA were higher in degP mutants than in wild-type cells. They were minimal in pldA mutants

  10. Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation.

    Science.gov (United States)

    Di Carlo, Dino; Jeong, Ki-Hun; Lee, Luke P

    2003-11-01

    A highly effective, reagentless, mechanical cell lysis device integrated in microfluidic channels is reported. Sample preparation, specifically cell lysis, is a critical element in 'lab-on-chip' applications. However, traditional methods of cell lysis require purification steps or complicated fabrication steps that a simple mechanical method of lysis may avoid. A simple and effective mechanical cell lysis system is designed, microfabricated, and characterized to quantify the efficiency of cell lysis and biomolecule accessibility. The device functionality is based on a microfluidic filter region with nanostructured barbs created using a modified deep reactive ion etching process. Mechanical lysis is characterized by using a membrane impermeable dye. Three main mechanisms of micro-mechanical lysis are described. Quantitative measurements of accessible protein as compared to a chemically lysed sample are acquired with optical absorption measurements at 280 and 414 nm. At a flow rate of 300 microL min(-1) within the filter region total protein and hemoglobin accessibilities of 4.8% and 7.5% are observed respectively as compared to 1.9% and 3.2% for a filter without nanostructured barbs.

  11. Chemical Cell Lysis System Applicable to Lab-on-a-Disc.

    Science.gov (United States)

    Lim, Dayeseul; Yoo, Jae Chern

    2017-09-01

    The design and fabrication of a heating system has been a significant challenge in implementing chemical lysis on a lab-on-a-disc (LOD). The proposed system contains a sample inlet, phase change material (PCM) array, heating chamber, and valve in a single disc, providing cost-effective, rapid, and fully automated chemical cell lysis. Compared to the conventional cell lysis system, our cell lysis system has many advantages, such as a compact structure that is easily integrated into the LOD and reduced processing time and labor. The experiments are conducted with Salmonella typhimurium strains to demonstrate the performance. The experimental results show that the proposed approach is greatly effective in realizing a chemical cell lysis system on an LOD with higher throughput in terms of purity and yield of DNA.

  12. Stretching single fibrin fibers hampers their lysis.

    Science.gov (United States)

    Li, Wei; Lucioni, Tomas; Li, Rongzhong; Bonin, Keith; Cho, Samuel S; Guthold, Martin

    2017-09-15

    Blood clots, whose main structural component is a mesh of microscopic fibrin fibers, experience mechanical strain from blood flow, clot retraction and interactions with platelets and other cells. We developed a transparent, striated and highly stretchable substrate made from fugitive glue (a styrenic block copolymer) to investigate how mechanical strain affects lysis of single, suspended fibrin fibers. In this suspended fiber assay, lysis manifested itself by fiber elongation, thickening (disassembly), fraying and collapse. Stretching single fibrin fibers significantly hampered their lysis. This effect was seen in uncrosslinked and crosslinked fibers. Crosslinking (without stretching) also hampered single fiber lysis. Our data suggest that strain is a novel mechanosensitive factor that regulates blood clot dissolution (fibrinolysis) at the single fiber level. At the molecular level of single fibrin molecules, strain may distort, or hinder access to, plasmin cleavage sites and thereby hamper lysis. Fibrin fibers are the major structural component of a blood clot. We developed a highly stretchable substrate made from fugitive glue and a suspended fibrin fiber lysis assay to investigate the effect of stretching on single fibrin fibers lysis. The key findings from our experiments are: 1) Fibers thicken and elongate upon lysis; 2) stretching strongly reduces lysis; 3) this effect is more pronounced for uncrosslinked fibers; and 4) stretching fibers has a similar effect on reducing lysis as crosslinking fibers. At the molecular level, strain may distort plasmin cleavage sites, or restrict access to those sites. Our results suggest that strain may be a novel mechanobiological factor that regulates fibrinolysis. Copyright © 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

  13. Low-Cost Energy-Efficient 3-D Nano-Spikes-Based Electric Cell Lysis Chips

    KAUST Repository

    Riaz, Kashif

    2017-05-04

    Electric cell lysis (ECL) is a promising technique to be integrated with portable lab-on-a-chip without lysing agent due to its simplicity and fast processing. ECL is usually limited by the requirements of high power/voltage and costly fabrication. In this paper, we present low-cost 3-D nano-spikes-based ECL (NSP-ECL) chips for efficient cell lysis at low power consumption. Highly ordered High-Aspect-Ratio (HAR). NSP arrays with controllable dimensions were fabricated on commercial aluminum foils through scalable and electrochemical anodization and etching. The optimized multiple pulse protocols with minimized undesirable electrochemical reactions (gas and bubble generation), common on micro parallel-plate ECL chips. Due to the scalability of fabrication process, 3-D NSPs were fabricated on small chips as well as on 4-in wafers. Phase diagram was constructed by defining critical electric field to induce cell lysis and for cell lysis saturation Esat to define non-ECL and ECL regions for different pulse parameters. NSP-ECL chips have achieved excellent cell lysis efficiencies ηlysis (ca 100%) at low applied voltages (2 V), 2~3 orders of magnitude lower than that of conventional systems. The energy consumption of NSP-ECL chips was 0.5-2 mJ/mL, 3~9 orders of magnitude lower as compared with the other methods (5J/mL-540kJ/mL). [2016-0305

  14. Microfluidic device for acoustic cell lysis

    Science.gov (United States)

    Branch, Darren W.; Cooley, Erika Jane; Smith, Gennifer Tanabe; James, Conrad D.; McClain, Jaime L.

    2015-08-04

    A microfluidic acoustic-based cell lysing device that can be integrated with on-chip nucleic acid extraction. Using a bulk acoustic wave (BAW) transducer array, acoustic waves can be coupled into microfluidic cartridges resulting in the lysis of cells contained therein by localized acoustic pressure. Cellular materials can then be extracted from the lysed cells. For example, nucleic acids can be extracted from the lysate using silica-based sol-gel filled microchannels, nucleic acid binding magnetic beads, or Nafion-coated electrodes. Integration of cell lysis and nucleic acid extraction on-chip enables a small, portable system that allows for rapid analysis in the field.

  15. Increased Resistance to osmotic lysis of sickled erythrocytes ...

    African Journals Online (AJOL)

    treated with CNw had significantly reduced osmotic lysis when compared with the untreated set (P<0.05, respectively) at various hypotonic NaCl concentrations. Various Hb genotypes exhibited a graded increase in osmotic pressure lysis in ...

  16. Comparison of lysis-centrifugation with lysis-filtration and a conventional unvented bottle for blood cultures.

    OpenAIRE

    Gill, V J; Zierdt, C H; Wu, T C; Stock, F; Pizzo, P A; MacLowry, J D

    1984-01-01

    Evaluation of a commercially available lysis-centrifugation blood culture system (Isolator, DuPont Co., Wilmington, Del.) and a lysis-filtration blood culture system for 3,111 cultures showed that both methods had comparable recoveries (73 and 68%, respectively) of significant aerobic and facultatively anaerobic isolates. The unvented conventional blood culture bottle had a recovery rate of 59%. Although the lysis-centrifugation and lysis-filtration systems had comparable recoveries of pathog...

  17. Miniature acoustic wave lysis system and uses thereof

    Science.gov (United States)

    Branch, Darren W.; Vreeland, Erika Cooley; Smith, Gennifer Tanabe

    2016-12-06

    The present invention relates to an acoustic lysis system including a disposable cartridge that can be reversibly coupled to a platform having a small, high-frequency piezoelectric transducer array. In particular, the system releases viable DNA, RNA, and proteins from human or bacterial cells, without chemicals or additional processing, to enable high-speed sample preparation for clinical point-of-care medical diagnostics and use with nano/microfluidic cartridges. Also described herein are methods of making and using the system of the invention.

  18. STUDIES ON THE BACTERIOPHAGE OF D'HERELLE : IX. EVIDENCE OF HYDROLYSIS OF BACTERIAL PROTEIN DURING LYSIS.

    Science.gov (United States)

    Hetler, D M; Bronfenbrenner, J

    1928-07-31

    1. During the process of lysis by bacteriophage, there is an appreciable increase in the amount of free amino acid present in the culture. 2. The increase of free amino acid is due to hydrolysis of bacterial protein.

  19. Micro Corona Ionizer as an Ozone Source for Bacterial Cell Lysis

    Science.gov (United States)

    Lee, Eun-Hee; Lim, Hyun Jeong; Chua, Beelee; Son, Ahjeong

    2015-04-01

    DNA extraction is a critical process of DNA assays including polymerase chain reaction (PCR), microarrays, molecular cloning, and DNA hybridization which has been well established and can be implemented by commercial kits. DNA extraction involves cell lysis, precipitation, and purification through the combination of physical and chemical processes. Cell lysis is essential to high DNA recovery yield which can be achieved via a variety of physical, chemical, and enzymatic methods. However, these methods were originally developed for bioassays that were labor intensive, time consuming, and vulnerable to contamination and inhibition. Here, we proposed to employ a micro corona ionizer as an ozone source to lyse bacterial cells. Ozone has been well known and used as a disinfectant which allows cell lysis and DNA extraction. Previously, we have shown that a micro corona ionizer is capable of generating a significant amount of ozone. In this study, we employed the micro corona ionizer for the bacterial cell lysis which consists of a 50 μm diameter cantilever wire as the discharge cathode and a 50 μm thick copper foil as anode. Applied voltages varied from 1900 to 2200 V with corresponding corona currents from 16 to 28 μA. The resultant ozone (concentration > 0.14 ppm) generated from the micro corona ionizer was bubbled into the sample via a miniature pump. We demonstrated the cell lysis of Pseudomonas putida as the target bacterium using the micro corona ionizer. At a flow rate of 38 ml/min and applied corona voltage of 2000 V, 98.5 ± 0.2% lysis (normalized to sonication result) was achieved after 10 min. In comparison, untreated and air-treated samples showed normalized % lysis of 11.9 ± 2.4 and 36.1 ± 1.7%, respectively. We also showed that the cell lysis efficiency could be significantly increased by increasing the flow rate and the applied corona voltage. By comparing the experimental results for continuous and pulsed treatment, we verified that the percentage of

  20. The euglobulin clot lysis time to assess the impact of nanoparticles on fibrinolysis

    Energy Technology Data Exchange (ETDEWEB)

    Minet, Valentine, E-mail: valentine.minet@unamur.be; Alpan, Lutfiye; Mullier, François [University of Namur – UNamur, Department of Pharmacy, Namur Thrombosis and Hemostasis Center (NTHC), Namur Nanosafety Center (NNC), NAmur Research Institute for Life Sciences NARILIS (Belgium); Toussaint, Olivier [Laboratory of Cellular Biochemistry and Biology (URBC) (Belgium); Lucas, Stéphane [University of Namur (UNamur), Research Centre for the Physics of Matter and Radiation (PMR-LARN), Namur Nanosafety Center NNC, NAmur Research Institute for Life Sciences NARILIS (Belgium); Dogné, Jean-Michel; Laloy, Julie, E-mail: julie.laloy@unamur.be [University of Namur – UNamur, Department of Pharmacy, Namur Thrombosis and Hemostasis Center (NTHC), Namur Nanosafety Center (NNC), NAmur Research Institute for Life Sciences NARILIS (Belgium)

    2015-07-15

    Nanoparticles (NPs) are developed for many applications in various fields, including nanomedicine. The NPs used in nanomedicine may disturb homeostasis in blood. Secondary hemostasis (blood coagulation) and fibrinolysis are complex physiological processes regulated by activators and inhibitors. An imbalance of this system can either lead to the development of hemorrhages or thrombosis. No data are currently available on the impact of NPs on fibrinolysis. The objectives of this study are (1) to select a screening test to study ex vivo the impact of NPs on fibrinolysis and (2) to test NPs with different physicochemical properties. Euglobulin clot lysis time test was selected to screen the impact of some NPs on fibrinolysis using normal pooled plasma. A dose-dependent decrease in the lysis time was observed with silicon dioxide and silver NPs without disturbing the fibrin network. Carbon black, silicon carbide, and copper oxide did not affect the lysis time at the tested concentrations.

  1. Pressure-mediated reduction of ultrasonically induced cell lysis

    International Nuclear Information System (INIS)

    Ciaravino, V.E.; Miller, M.W.; Carstensen, E.L.

    1981-01-01

    Chinese hamster V-79 cells, exposed in polystyrene tubes for 5 min to 1-MHz continuous-wave ultrasound, were lysed more by a 10 than a 5 W/cm 2 intensity. Higher atmospheric pressure was needed to eliminate lysis with the former relative to the latter intensity, but lysis by 10 W/cm 2 was completely climinated with 2 atm of hydrostatic pressure. The reduction in lysis per unit increase in atmospheric pressure was comparable for both ultrasound intensities

  2. Solubilization of proteins: the importance of lysis buffer choice.

    Science.gov (United States)

    Peach, Mandy; Marsh, Noelle; Miskiewicz, Ewa I; MacPhee, Daniel J

    2015-01-01

    The efficient extraction of proteins of interest from cells and tissues is not always straightforward. Here we demonstrate the differences in extraction of the focal adhesion protein Kindlin-2 from choriocarcinoma cells using NP-40 and RIPA lysis buffer. Furthermore, we demonstrate the use of a more denaturing urea/thiourea lysis buffer for solubilization, by comparing its effectiveness for solubilization of small heat-shock proteins from smooth muscle with the often utilized RIPA lysis buffer. Overall, the results demonstrate the importance of establishing the optimal lysis buffer for specific protein solubilization within the experimental workflow.

  3. Low-Cost Energy-Efficient 3-D Nano-Spikes-Based Electric Cell Lysis Chips

    KAUST Repository

    Riaz, Kashif; Leung, Siu; Fan, Zhiyong; Lee, Yi-Kuen

    2017-01-01

    Electric cell lysis (ECL) is a promising technique to be integrated with portable lab-on-a-chip without lysing agent due to its simplicity and fast processing. ECL is usually limited by the requirements of high power/voltage and costly fabrication

  4. Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate

    Science.gov (United States)

    Culbertson, Christopher T [Oak Ridge, TN; Jacobson, Stephen C [Knoxville, TN; McClain, Maxine A [Knoxville, TN; Ramsey, J Michael [Knoxville, TN

    2008-09-02

    Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.

  5. 21 CFR 864.7275 - Euglobulin lysis time tests.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Euglobulin lysis time tests. 864.7275 Section 864.7275 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Kits and Packages § 864.7275 Euglobulin lysis...

  6. Integration of nanoparticle cell lysis and microchip PCR for one-step rapid detection of bacteria.

    Science.gov (United States)

    Wan, Weijie; Yeow, John T W

    2012-04-01

    This paper describes an integrated microchip system as an efficient and cost-effective solution involving Nanotechnology and Lab-on-a-Chip technology for the rapid detection of bacteria. The system is based on using surface-modified gold nanoparticles for efficient cell lysis followed by microchip PCR without having to remove the nanoparticles from the PCR solution. Poly(quaternary ammonium) modified gold nanoparticles are used to provide a novel and efficient cell lysis method without the need to go through time-consuming, expensive and complicated microfabrication processes as most of current cell lysis methods for Lab-on-a-Chip applications do. It also facilitates the integration of cell lysis and PCR by sharing the same reaction chamber as PCR uses. It is integrated with a prototype microchip PCR system consisting of a physical microchip PCR device and an automated temperature control mechanism. The research work explores solutions for the problem of PCR inhibition caused by gold nanoparticles as well as for the problem of non-specific PCR amplification in the integrated microchip system. It also explores the possibility of greatly reducing PCR cycling time to achieve the same result compared to the protocol for a regular PCR machine. The simplicity of the setup makes it easy to be integrated with other Lab-on-a-Chip functional modules to create customized solutions for target applications.

  7. Squamous cell carcinoma complicating an hereditary epidermo-lysis bullosa

    International Nuclear Information System (INIS)

    Mseddi, M.; Turki, H.; Marrekchi, S.; Abdelmaksoud, W.; Masmoudi, A.; Bouassida, S.; Zahaf, A.

    2004-01-01

    The dystrophic form of hereditary epidermo-lysis bullosa is associated with an increased frequency of squamous cell carcinoma. We report a new case. An 18-year-old patient, carrying a Hallopeau Siemens hereditary epidermo-lysis bullosa, presented a subcutaneous nodular lesion, for 1 year that ulcerated and budded with inguinal lymphadenopathy. The histological study ted to the conclusion of a well differentiated squamous cell carcinoma. The patient was treated surgically. Tumor and metastatic lymph nodes were excised. A radiotherapy was decided but the postoperative course was fatal due to an infection and to a deterioration of her general condition. Squamous cell carcinoma frequently occurs on the cicatricial lesion of hereditary epidermo-lysis bullosa and usually affects males with recessive hereditary epidermo-lysis bullosa. Metastases are frequent, precocious and multiple. The treatment may be surgical. The particularities of our observation are the young age of patient and the localization. (author)

  8. Urea enhances cell lysis of Schizosaccharomyces pombe ura4 mutants.

    Science.gov (United States)

    Nishino, Kohei; Kushima, Misaki; Kaino, Tomohiro; Matsuo, Yasuhiro; Kawamukai, Makoto

    2017-07-01

    Cell lysis is induced in Schizosaccharomyces pombe ∆ura4 cells grown in YPD medium, which contains yeast extract, polypeptone, and glucose. To identify the medium components that induce cell lysis, we first tested various kinds of yeast extracts from different suppliers. Cell lysis of ∆ura4 cells on YE medium was observed when yeast extracts from OXOID, BD, Oriental, and Difco were used, but not when using yeast extract from Kyokuto. To determine which compounds induced cell lysis, we subjected yeast extract and polypeptone to GC-MS analysis. Ten kinds of compounds were detected in OXOID and BD yeast extracts, but not in Kyokuto yeast extract. Among them was urea, which was also present in polypeptone, and it clearly induced cell lysis. Deletion of the ure2 gene, which is responsible for utilizing urea, abolished the lytic effect of urea. The effect of urea was suppressed by deletion of pub1, and a similar phenotype was observed in the presence of polypeptone. Thus, urea is an inducer of cell lysis in S. pombe ∆ura4 cells.

  9. Electrical lysis: dynamics revisited and advances in On-chip operation.

    Science.gov (United States)

    Morshed, Bashir; Shams, Maitham; Mussivand, Tofy

    2013-01-01

    Electrical lysis (EL) is the process of breaking the cell membrane to expose the internal contents under an applied high electric field. Lysis is an important phenomenon for cellular analysis, medical treatment, and biofouling control. This paper aims to review, summarize, and analyze recent advancements on EL. Major databases including PubMed, Ei Engineering Village, IEEE Xplore, and Scholars Portal were searched using relevant keywords. More than 50 articles published in English since 1997 are cited in this article. EL has several key advantages compared to other lysis techniques such as chemical, mechanical, sonication, or laser, including rapid speed of operation, ability to control, miniaturization, low cost, and low power requirement. A variety of cell types have been investigated for including protoplasts, E. coli, yeasts, blood cells, and cancer cells. EL has been developed and applied for decontamination, cytology, genetics, single-cell analysis, cancer treatment, and other applications. On-chip EL is a promising technology for multiplexed automated implementation of cell-sample preparation and processing with micro- or nanoliter reagents.

  10. Rapid kinetics of lysis in human natural cell-mediated cytotoxicity: some implications

    International Nuclear Information System (INIS)

    Bloom, E.T.; Babbitt, J.T.

    1983-01-01

    The entire lytic process of natural cell-mediated cytotoxicity against sensitive target cells can occur rapidly, within minutes. This was demonstrated by 51 chromium release and in single-cell assays. At the cellular level, most of the target cell lysis occurred within 15-30 min after binding to effector cells. The enriched natural killer cell subpopulation of lymphocytes obtained by Percoll density gradient centrifugation (containing greater than 70% large granular lymphocytes (LGL)) was the most rapidly lytic population by 51 chromium release. However, in the single-cell assay, the rate of lysis of bound target cells was quite similar for the LGL-enriched effector subpopulation and the higher density subpopulation of effector cells recognized previously. Both the light and dense effector cells contained similar numbers of target binding cells. Therefore, that the light subpopulation effected lysis more rapidly and to a greater extent than the dense subpopulation suggested that the low-density effector cells probably recycled more rapidly than those of higher density. This was corroborated by the finding that when conjugates were formed at 29 degrees C for the single-cell assay, a significant number of dead unconjugated targets could be observed only on the slides made with the LGL-enriched effector cells but not on those made with dense effector cell. Lysis continued to increase in the chromium-release assay probably because of recycling, recruitment, and/or heterogeneity of the effector cells, and/or because of heterogeneity or delayed death of the target cells

  11. Role of the SRRz/Rz1 lambdoid lysis cassette in the pathoadaptive evolution of Shigella.

    Science.gov (United States)

    Leuzzi, Adriano; Grossi, Milena; Di Martino, Maria Letizia; Pasqua, Martina; Micheli, Gioacchino; Colonna, Bianca; Prosseda, Gianni

    2017-06-01

    Shigella, the etiological agent of bacillary dysentery (shigellosis), is a highly adapted human pathogen. It evolved from an innocuous ancestor resembling the Escherichia coli strain by gain and loss of genes and functions. While the gain process concerns the acquisition of the genetic determinants of virulence, the loss is related to the adaptation of the genome to the new pathogenic status and occurs by pathoadaptive mutation of antivirulence genes. In this study, we highlight that the SRRz/Rz 1 lambdoid lysis cassette, even though stably adopted in E. coli K12 by virtue of its beneficial effect on cell physiology, has undergone a significant decay in Shigella. Moreover, we show the antivirulence nature of the SRRz/Rz 1 lysis cassette in Shigella. In fact, by restoring the SRRz/Rz 1 expression in this pathogen, we observe an increased release of peptidoglycan fragments, causing an unbalance in the fine control exerted by Shigella on host innate immunity and a mitigation of its virulence. This strongly affects the virulence of Shigella and allows to consider the loss of SRRz/Rz 1 lysis cassette as another pathoadaptive event in the life of Shigella. Copyright © 2017 Elsevier GmbH. All rights reserved.

  12. Inhibition of pneumococcal autolysis in lysis-centrifugation blood culture.

    OpenAIRE

    Lehtonen, O P

    1986-01-01

    The recovery of Streptococcus pneumoniae from the Isolator lysis-centrifugation blood culture has been low in many studies. The poor survival of pneumococci was not due to toxicity of the Isolator medium but to autolysis before plating. This autolysis was completely inhibited by adding 10 mM phosphorylcholine to the Isolator medium.

  13. effect of natural blue-green algal cells lysis on freshwater quality

    African Journals Online (AJOL)

    Compaq

    released into water due to algal cells lysis was performed by placing samples in two ... Keywords; Algae, cells lysis, Fatty acids, gas chromatography time-of-flight mass spectrometry, water quality ... Factors such as municipal and industrial.

  14. Surgical treatment of bilateral nondisplaced isthmic lysis by interlaminar fixation device

    Directory of Open Access Journals (Sweden)

    Keyvan Mostofi

    2017-01-01

    Full Text Available Study Design: Spondylolysis is a defect in the portion of pars interarticularis. The latter affects approximately 6% of the population. It is caused by repetitive trauma in hyperextension. Low back pain is the most common symptom. Methods: We implanted interspinous process devices in 12 patients with isthmic lysis without spondylolisthesis for low back pain. The purpose of the surgery was to conduct a minimally invasive procedure. Results: In eight cases, patients became asymptomatic. In two cases, there has been a considerable improvement. In two cases, no change had been noted. Conclusion: This good result motivates us to consider this approach a part of therapeutic arsenal for some cases of spondylolysis.

  15. Direct Cellular Lysis/Protein Extraction Protocol for Soil Metaproteomics

    Energy Technology Data Exchange (ETDEWEB)

    Chourey, Karuna [ORNL; Jansson, Janet [Lawrence Berkeley National Laboratory (LBNL); Verberkmoes, Nathan C [ORNL; Shah, Manesh B [ORNL; Chavarria, Krystle L. [Lawrence Berkeley National Laboratory (LBNL); Tom, Lauren M [Lawrence Berkeley National Laboratory (LBNL); Brodie, Eoin L. [Lawrence Berkeley National Laboratory (LBNL); Hettich, Robert {Bob} L [ORNL

    2010-01-01

    We present a novel direct protocol for deep proteome characterization of microorganisms in soil. The method employs thermally assisted detergent-based cellular lysis (SDS) of soil samples, followed by TCA precipitation for proteome extraction/cleanup prior to liquid chromatography-mass spectrometric characterization. This approach was developed and optimized using different soils inoculated with genome-sequenced bacteria (Gram-negative Pseudomonas putida or Gram-positive Arthrobacter chlorophenolicus). Direct soil protein extraction was compared to protein extraction from cells isolated from the soil matrix prior to lysis (indirect method). Each approach resulted in identification of greater than 500 unique proteins, with a wide range in molecular mass and functional categories. To our knowledge, this SDS-TCA approach enables the deepest proteome characterizations of microbes in soil to date, without significant biases in protein size, localization, or functional category compared to pure cultures. This protocol should provide a powerful tool for ecological studies of soil microbial communities.

  16. Dark membrane lysis and photosensitization by 3-carbethoxypsoralen

    Energy Technology Data Exchange (ETDEWEB)

    Muller-Runkel, R.; Grossweiner, L.I. (Illinois Inst. of Tech., Chicago (USA). Dept. of Physics)

    1981-03-01

    Aqueous solutions of 3-carbethoxypsoralen (3-CPs) induced lysis of egg lecithin liposomes and whole human erythrocytes in the dark. Near-UV irradiation of 3-CPs sensitized the inactivation of lysozyme attributed to the production of reactive radical intermediates. The implications of these findings for the use of 3-CPs as a sensitizer in psoralen + UV-A (PUVA) therapy of psoriasis are discussed.

  17. Dark membrane lysis and photosensitization by 3-carbethoxypsoralen

    International Nuclear Information System (INIS)

    Muller-Runkel, R.; Grossweiner, L.I.

    1981-01-01

    Aqueous solutions of 3-carbethoxypsoralen (3-CPs) induced lysis of egg lecithin liposomes and whole human erythrocytes in the dark. Near-UV irradiation of 3-CPs sensitized the inactivation of lysozyme attributed to the production of reactive radical intermediates. The implications of these findings for the use of 3-CPs as a sensitizer in psoralen + UV-A (PUVA) therapy of psoriasis are discussed. (author)

  18. Bacterial Infochemicals are Drivers of Algal Lysis

    Science.gov (United States)

    Whalen, K.; Deering, R.; Rowley, D. C.; El Gamal, A.; Schorn, M.; Moore, B. S.; Johnson, M. D.; Mincer, T. J.; Harvey, E.

    2016-02-01

    Processing of organic matter by bacteria forces oceanic biogeochemical cycles, food web structure and ultimately environmental stoichiometry. A newly emerging picture of the microbial loop suggests that bacteria are not merely passive recipients of dissolved organic matter (DOM) from phytoplankton exudate. Rather, heterotrophic bacteria can mediate the flow of DOM by actively producing soluble algicidal compounds. However, deciphering those chemical signals that determine these interactions has remained a challenge. Here, we report the isolation of 2-heptyl-4-quinolone (HHQ), released by Pseudoalteromonas piscicida, a marine gamma-proteobacteria isolated from plastic debris in the North Atlantic. Both 2-heptyl-3-hydroxy-4-quinolone and its immediate precursor, HHQ are known to function as antibiotics and quorum sensing signaling molecules with crucial roles in virulence, and apoptosis in eukaryotic cells (e.g. fungi and mammalian cells). Our ecologically-relevant screening of live cells and filtrate from P. piscicida cultures caused a significant decrease in the growth rate of the bloom-forming coccolithophore, Emiliania huxleyi. Bioassay-guided fraction of P. piscicida extracellular crude extracts identified HHQ, which induced mortality in three strains of E. huxleyi with an IC50 in the nanomolar range. In contrast, the marine chlorophyte, Dunaliella tertiolecta and diatom, Phaeodactylum tricornutum were unaffected by HHQ exposures (IC50 > 10 micromolar), but were susceptible to extracts of P. piscicida, indicating this bacterium may produce a cocktail of algicidal compounds specific to different phytoplankton guilds. The ability of HHQ to influence phytoplankton growth suggests that alkylquinolone-signaling molecules play a fundamental role in interkingdom interactions, ultimately influencing shifts in phytoplankton population dynamics. This study implicates a new role for HHQ beyond its importance in quorum sensing.

  19. Impaired respiration elicits SrrAB-dependent programmed cell lysis and biofilm formation in Staphylococcus aureus

    Science.gov (United States)

    Mashruwala, Ameya A; van de Guchte, Adriana; Boyd, Jeffrey M

    2017-01-01

    Biofilms are communities of microorganisms attached to a surface or each other. Biofilm-associated cells are the etiologic agents of recurrent Staphylococcus aureus infections. Infected human tissues are hypoxic or anoxic. S. aureus increases biofilm formation in response to hypoxia, but how this occurs is unknown. In the current study we report that oxygen influences biofilm formation in its capacity as a terminal electron acceptor for cellular respiration. Genetic, physiological, or chemical inhibition of respiratory processes elicited increased biofilm formation. Impaired respiration led to increased cell lysis via divergent regulation of two processes: increased expression of the AtlA murein hydrolase and decreased expression of wall-teichoic acids. The AltA-dependent release of cytosolic DNA contributed to increased biofilm formation. Further, cell lysis and biofilm formation were governed by the SrrAB two-component regulatory system. Data presented support a model wherein SrrAB-dependent biofilm formation occurs in response to the accumulation of reduced menaquinone. DOI: http://dx.doi.org/10.7554/eLife.23845.001 PMID:28221135

  20. Estudio EPIFARM

    Directory of Open Access Journals (Sweden)

    Martín Morales A

    2010-12-01

    Full Text Available INTRODUCCIÓN Los farmacéuticos comunitarios pueden ser un importante primer punto de contacto con los pacientes con disfunción eréctil (DE, pero hasta la fecha no hay ningún estudio sobre las características de los hombres que acuden a un farmacéutico solicitando consejo o tratamiento para la DE. OBJETIVO Caracterizar los perfiles de los hombres que solicitan tratamiento para la DE en la farmacia, con o sin receta de inhibidores de la fosfodiesterasa tipo 5 (iPDE5. MÉTODOS Entre septiembre y noviembre de 2008 se realizó un estudio observacional, transversal y multicéntrico en farmacias comunitarias de España. De aquellos hombres que solicitaban consejo o tratamiento para la DE, cada investigador reclutó un paciente que tenía receta médica de iPDE5 y otro que acudía sin receta médica. Los farmacéuticos del estudio completaron un cuestionario de datos demográficos, clínicos y conductuales del paciente, incluido el Cuestionario de salud sexual para varones (Sexual Health Inventory for Men. VARIABLES PRINCIPALES Características demográficas y respuestas a los cuestionarios. RESULTADOS 574 farmacéuticos seleccionaron a 1.147 pacientes, de los cuales 1.113 fueron incluidos en el análisis. No se observaron diferencias estadísticas entre los grupos en cuanto al peso, la hipertensión, la diabetes mellitus, la hipercolesterolemia, la dislipidemia, la depresión o el estrés. Tampoco se observaron diferencias estadísticas respecto a la gravedad de la DE (p = 0,7892 ni a la proporción de hombres sin DE en cada grupo (p = 0,5755. En ambos grupos, los pacientes habían presentado síntomas de DE durante una media de veintiséis meses antes de la primera consulta a un profesional sanitario. Para el 60,2% de los pacientes incluidos en el grupo sin receta, la visita a la farmacia fue la primera ocasión en la que habían hablado de su DE con un profesional sanitario, y el 50% de aquellos que habían hablado previamente de la DE lo hab

  1. Aggregate Formation During the Viral Lysis of a Marine Diatom

    Directory of Open Access Journals (Sweden)

    Yosuke Yamada

    2018-05-01

    Full Text Available Recent studies have suggested that the viral lysis of microbes not only facilitates the conversion of particulate organic matter into dissolved organic matter, but also promotes the formation of organic aggregates, which enhance the export of organic carbon from the surface ocean to the deep sea. However, experimental data supporting this proposition are limited. Here, we tested the hypothesis that the viral infection of marine diatoms enhances aggregate formation. We used a model system consisting of Chaetoceros tenuissimus, a bloom-forming diatom with an approximate cell size of 3–10 μm, and a DNA virus, CtenDNAV type II, which replicates in the nucleus of C. tenuissimus. The volume of large particles (50–400 μm in equivalent spherical diameters, determined from photographic images was measured over time (up to 15 days in the diatom-alone control and a virus-added diatom culture. We also determined the concentrations of Coomassie-stainable particles (CSP, proteinaceous particles and transparent exopolymeric particles (TEP, acid-polysaccharide-rich particles with colorimetric methods. The total volume of large particles was significantly higher (5–59 fold in the virus-added diatoms than in the diatom-alone control during the period in which the viral lysis of the diatoms proceeded. One class of large particles produced in the virus-added diatoms was flake-shaped. The flakes were tightly packed and dense, and sank rapidly, possibly playing an important role in the vertical delivery of materials from the surface to the deep sea. The bulk CSP concentrations tended to be higher in the virus-added diatoms than in the diatom-alone control, whereas the reverse was true for the TEP. These results suggest that proteinaceous polymers are involved in aggregate formation. Our data support the emerging notion that the viral lysis of microbes facilitates aggregate formation and the export of organic carbon in the ocean.

  2. A novel clot lysis assay for recombinant plasminogen activator.

    Science.gov (United States)

    Jamialahmadi, Oveis; Fazeli, Ahmad; Hashemi-Najafabadi, Sameereh; Fazeli, Mohammad Reza

    2015-03-01

    Recombinant plasminogen activator (r-PA, reteplase) is an engineered variant of alteplase. When expressed in E. coli, it appears as inclusion bodies that require refolding to recover its biological activity. An important step following refolding is to determine the activity of refolded protein. Current methods for enzymatic activity of thrombolytic drugs are costly and complex. Here a straightforward and low-cost clot lysis assay was developed. It quantitatively measures the activity of the commercial reteplase and is also capable of screening refolding conditions. As evidence for adequate accuracy and sensitivity of the current assay, r-PA activity measurements are shown to be comparable to those obtained from chromogenic substrate assay.

  3. Evaluation of Lysis Methods for the Extraction of Bacterial DNA for Analysis of the Vaginal Microbiota.

    Directory of Open Access Journals (Sweden)

    Christina Gill

    Full Text Available Recent studies on the vaginal microbiota have employed molecular techniques such as 16S rRNA gene sequencing to describe the bacterial community as a whole. These techniques require the lysis of bacterial cells to release DNA before purification and PCR amplification of the 16S rRNA gene. Currently, methods for the lysis of bacterial cells are not standardised and there is potential for introducing bias into the results if some bacterial species are lysed less efficiently than others. This study aimed to compare the results of vaginal microbiota profiling using four different pretreatment methods for the lysis of bacterial samples (30 min of lysis with lysozyme, 16 hours of lysis with lysozyme, 60 min of lysis with a mixture of lysozyme, mutanolysin and lysostaphin and 30 min of lysis with lysozyme followed by bead beating prior to chemical and enzyme-based DNA extraction with a commercial kit.After extraction, DNA yield did not significantly differ between methods with the exception of lysis with lysozyme combined with bead beating which produced significantly lower yields when compared to lysis with the enzyme cocktail or 30 min lysis with lysozyme only. However, this did not result in a statistically significant difference in the observed alpha diversity of samples. The beta diversity (Bray-Curtis dissimilarity between different lysis methods was statistically significantly different, but this difference was small compared to differences between samples, and did not affect the grouping of samples with similar vaginal bacterial community structure by hierarchical clustering.An understanding of how laboratory methods affect the results of microbiota studies is vital in order to accurately interpret the results and make valid comparisons between studies. Our results indicate that the choice of lysis method does not prevent the detection of effects relating to the type of vaginal bacterial community one of the main outcome measures of

  4. Evaluation of Lysis Methods for the Extraction of Bacterial DNA for Analysis of the Vaginal Microbiota.

    Science.gov (United States)

    Gill, Christina; van de Wijgert, Janneke H H M; Blow, Frances; Darby, Alistair C

    2016-01-01

    Recent studies on the vaginal microbiota have employed molecular techniques such as 16S rRNA gene sequencing to describe the bacterial community as a whole. These techniques require the lysis of bacterial cells to release DNA before purification and PCR amplification of the 16S rRNA gene. Currently, methods for the lysis of bacterial cells are not standardised and there is potential for introducing bias into the results if some bacterial species are lysed less efficiently than others. This study aimed to compare the results of vaginal microbiota profiling using four different pretreatment methods for the lysis of bacterial samples (30 min of lysis with lysozyme, 16 hours of lysis with lysozyme, 60 min of lysis with a mixture of lysozyme, mutanolysin and lysostaphin and 30 min of lysis with lysozyme followed by bead beating) prior to chemical and enzyme-based DNA extraction with a commercial kit. After extraction, DNA yield did not significantly differ between methods with the exception of lysis with lysozyme combined with bead beating which produced significantly lower yields when compared to lysis with the enzyme cocktail or 30 min lysis with lysozyme only. However, this did not result in a statistically significant difference in the observed alpha diversity of samples. The beta diversity (Bray-Curtis dissimilarity) between different lysis methods was statistically significantly different, but this difference was small compared to differences between samples, and did not affect the grouping of samples with similar vaginal bacterial community structure by hierarchical clustering. An understanding of how laboratory methods affect the results of microbiota studies is vital in order to accurately interpret the results and make valid comparisons between studies. Our results indicate that the choice of lysis method does not prevent the detection of effects relating to the type of vaginal bacterial community one of the main outcome measures of epidemiological studies

  5. Polymer Coatings in 3D-Printed Fluidic Device Channels for Improved Cellular Adherence Prior to Electrical Lysis.

    Science.gov (United States)

    Gross, Bethany C; Anderson, Kari B; Meisel, Jayda E; McNitt, Megan I; Spence, Dana M

    2015-06-16

    This paper describes the design and fabrication of a polyjet-based three-dimensional (3D)-printed fluidic device where poly(dimethylsiloxane) (PDMS) or polystyrene (PS) were used to coat the sides of a fluidic channel within the device to promote adhesion of an immobilized cell layer. The device was designed using computer-aided design software and converted into an .STL file prior to printing. The rigid, transparent material used in the printing process provides an optically transparent path to visualize endothelial cell adherence and supports integration of removable electrodes for electrical cell lysis in a specified portion of the channel (1 mm width × 0.8 mm height × 2 mm length). Through manipulation of channel geometry, a low-voltage power source (500 V max) was used to selectively lyse adhered endothelial cells in a tapered region of the channel. Cell viability was maintained on the device over a 5 day period (98% viable), though cell coverage decreased after day 4 with static media delivery. Optimal lysis potentials were obtained for the two fabricated device geometries, and selective cell clearance was achieved with cell lysis efficiencies of 94 and 96%. The bottleneck of unknown surface properties from proprietary resin use in fabricating 3D-printed materials is overcome through techniques to incorporate PDMS and PS.

  6. Lab-on-a-Disc Platform for Automated Chemical Cell Lysis.

    Science.gov (United States)

    Seo, Moo-Jung; Yoo, Jae-Chern

    2018-02-26

    Chemical cell lysis is an interesting topic in the research to Lab-on-a-Disc (LOD) platforms on account of its perfect compatibility with the centrifugal spin column format. However, standard procedures followed in chemical cell lysis require sophisticated non-contact temperature control as well as the use of pressure resistant valves. These requirements pose a significant challenge thereby making the automation of chemical cell lysis on an LOD extremely difficult to achieve. In this study, an LOD capable of performing fully automated chemical cell lysis is proposed, where a combination of chemical and thermal methods has been used. It comprises a sample inlet, phase change material sheet (PCMS)-based temperature sensor, heating chamber, and pressure resistant valves. The PCMS melts and solidifies at a certain temperature and thus is capable of indicating whether the heating chamber has reached a specific temperature. Compared to conventional cell lysis systems, the proposed system offers advantages of reduced manual labor and a compact structure that can be readily integrated onto an LOD. Experiments using Salmonella typhimurium strains were conducted to confirm the performance of the proposed cell lysis system. The experimental results demonstrate that the proposed system has great potential in realizing chemical cell lysis on an LOD whilst achieving higher throughput in terms of purity and yield of DNA thereby providing a good alternative to conventional cell lysis systems.

  7. Lab-on-a-Disc Platform for Automated Chemical Cell Lysis

    Directory of Open Access Journals (Sweden)

    Moo-Jung Seo

    2018-02-01

    Full Text Available Chemical cell lysis is an interesting topic in the research to Lab-on-a-Disc (LOD platforms on account of its perfect compatibility with the centrifugal spin column format. However, standard procedures followed in chemical cell lysis require sophisticated non-contact temperature control as well as the use of pressure resistant valves. These requirements pose a significant challenge thereby making the automation of chemical cell lysis on an LOD extremely difficult to achieve. In this study, an LOD capable of performing fully automated chemical cell lysis is proposed, where a combination of chemical and thermal methods has been used. It comprises a sample inlet, phase change material sheet (PCMS-based temperature sensor, heating chamber, and pressure resistant valves. The PCMS melts and solidifies at a certain temperature and thus is capable of indicating whether the heating chamber has reached a specific temperature. Compared to conventional cell lysis systems, the proposed system offers advantages of reduced manual labor and a compact structure that can be readily integrated onto an LOD. Experiments using Salmonella typhimurium strains were conducted to confirm the performance of the proposed cell lysis system. The experimental results demonstrate that the proposed system has great potential in realizing chemical cell lysis on an LOD whilst achieving higher throughput in terms of purity and yield of DNA thereby providing a good alternative to conventional cell lysis systems.

  8. Isolation of Mycobacterium chelonei with the lysis-centrifugation blood culture technique.

    OpenAIRE

    Fojtasek, M F; Kelly, M T

    1982-01-01

    Mycobacterium chelonei was isolated from a patient by the lysis-centrifugation and the conventional two-bottle blood culture methods. The lysis-centrifugation method was significantly more sensitive and rapid than the conventional method in detecting and isolating this organism; quantitations done by this method were useful for monitoring response to therapy.

  9. Droplet Microfluidics for Compartmentalized Cell Lysis and Extension of DNA from Single-Cells

    Science.gov (United States)

    Zimny, Philip; Juncker, David; Reisner, Walter

    Current single cell DNA analysis methods suffer from (i) bias introduced by the need for molecular amplification and (ii) limited ability to sequence repetitive elements, resulting in (iii) an inability to obtain information regarding long range genomic features. Recent efforts to circumvent these limitations rely on techniques for sensing single molecules of DNA extracted from single-cells. Here we demonstrate a droplet microfluidic approach for encapsulation and biochemical processing of single-cells inside alginate microparticles. In our approach, single-cells are first packaged inside the alginate microparticles followed by cell lysis, DNA purification, and labeling steps performed off-chip inside this microparticle system. The alginate microparticles are then introduced inside a micro/nanofluidic system where the alginate is broken down via a chelating buffer, releasing long DNA molecules which are then extended inside nanofluidic channels for analysis via standard mapping protocols.

  10. Clot formation and lysis in platelet rich plasma of healthy donors and patients with resistant hypertension

    Directory of Open Access Journals (Sweden)

    I. I. Patalakh

    2018-04-01

    Full Text Available Hemostatic balance in blood is affected by numerous factors, including coagulation and fibrinolytic proteins, the wide spectrum of their inhibitors, and blood cells. Since platelets can participate in contradictory processes, they significantly complicate the whole picture. Therefore, nowadays the development of global assays of hemostasis, which can reflect the physiological process of hemostasis and can be used for point-of-care diagnosis of thrombosis, is crucial. This paper outlines a new approach we used to analyze the capabilities of clot waveform analysis tools to distinguish the response of platelet-rich plasma from healthy donors and patients with arterial hypertension caused by stimulation of coagulation and lysis (with exogenous thrombin and recombinant tissue-type plasminogen activator, respectively. In donor plasma, when the clot degradation was accompanied by 40 IU/ml of recombinant tissue-type plasminogen activator, platelets potentiated fibrinolysis more than coagulation, which ultimately shifts the overall balance to a profibrinolytic state. At the same time, for patients with hypertension, platelets, embedded in clot obtained from platelet-rich plasma, showed a weaker ability to stimulate fibrinolysis. The obtained data gives the evidence that platelets can act not only as procoagulants but also as profibrinolytics. By simultaneously amplifying coagulation and fibrinolysis, making their rates comparable, platelets would control plasma procoagulant activity, thereby regulating local hemostatic balance, the size and lifetime of the clot. Moreover, clot waveform analysis may be used to distinguish the effects of platelet-rich plasma on clotting or lysis of fibrin clots in healthy donors and patients with essential hypertension.

  11. Tratamiento estadístico de la falta de respuesta en estudios epidemiológicos transversales Statistical processing of non-response in transversal epidemiological studies

    Directory of Open Access Journals (Sweden)

    Eduardo Carracedo-Martínez

    2006-08-01

    Full Text Available En los estudios epidemiológicos, la falta de respuestas constituye una gran limitación por la pérdida de validez y el poder estadístico que implica, sea que se produzca en forma de participación parcial (el sujeto deja alguna pregunta sin contestar o como ausencia de participación (el individuo no contesta ninguna pregunta. En este trabajo se realiza una revisión de la bibliografía científica sobre los distintos métodos para realizar un tratamiento estadístico de los datos cuando no se obtienen respuestas; la finalidad es contrarrestar las limitaciones de la información, siempre en el caso de estudios epidemiológicos no longitudinales. La mayor parte de los métodos estadísticos se centra en el tratamiento de la participación parcial (datos faltantes y puede clasificarse en dos grandes grupos: de asignación y de datos completos. La elección correcta del método para un estudio requiere un análisis previo de la matriz de los datos, en relación con el mecanismo de generación de datos faltantes y el porcentaje del total de los datos que representa.In epidemiological surveys, non-response constitutes a great limitation due to the loss of validity and statistical power it represents, whether such a loss occurs due to partial participation (the individual fails to answer certain variables or due to total lack of participation (the individual does not answer any variable. This paper reviews the scientific literature on the different methods to process statistic data when non-response has occurred in non-longitudinal studies, so as to counteract their effect in such studies. Most statistical methods focus on dealing with partial participation (missing data. These methods, of which there is a great variety, can be classified into two large groups: imputation and complete data. For accurate selection of the study method, it is necessary to study the data matrix beforehand, observing the missing data generation mechanism, as well as the

  12. Tumor lysis syndrome in a patient with metastatic colon cancer after treatment with oxaliplatin and 5-Fu

    Directory of Open Access Journals (Sweden)

    Ruo-Han Tseng

    2016-12-01

    Full Text Available Tumor lysis syndrome in solid tumors is a rare occurrence, with a poor prognosis. We present the case of a patient of recurrent colon cancer who received chemotherapy with FOLFOX regimen (lencovorin, fluorouracil, and oxaliplatin with subsequent tumor lysis. We present a recurrent rectal cancer patient suffered from tumor lysis syndrome after salvage FOLFOX regimen. After treat with CVVH with improved conscious status. In this case report, we had review the tumor lysis in solid tumor.

  13. Lysis of Chlamydomonas reinhardtii by high-intensity focused ultrasound as a function of exposure time.

    Science.gov (United States)

    Bigelow, Timothy A; Xu, Jin; Stessman, Dan J; Yao, Linxing; Spalding, Martin H; Wang, Tong

    2014-05-01

    Efficient lysis of microalgae for lipid extraction is an important concern when processing biofuels. Historically, ultrasound frequencies in the range of 10-40 kHz have been utilized for this task. However, greater efficiencies might be achievable if higher frequencies could be used. In our study, we evaluated the potential of using 1.1 MHz ultrasound to lyse microalgae for biofuel production while using Chlamydomonas reinhardtii as a model organism. The ultrasound was generated using a spherically focused transducer with a focal length of 6.34 cm and an active diameter of 6.36 cm driven by 20 cycle sine-wave tone bursts at a pulse repetition frequency of 2 kHz (3.6% duty cycle). The time-average acoustic power output was 26.2 W while the spatial-peak-pulse-average intensity (ISPPA) for each tone burst was 41 kW/cm(2). The peak compressional and rarefactional pressures at the focus were 102 and 17 MPa, respectively. The exposure time was varied for the different cases in the experiments from 5s to 9 min and cell lysis was assessed by quantifying the percentage of protein and chlorophyll release into the supernate as well as the lipid extractability. Free radical generation and lipid oxidation for the different ultrasound exposures were also determined. We found that there was a statistically significant increase in lipid extractability for all of the exposures compared to the control. The longer exposures also completely fragmented the cells releasing almost all of the protein and chlorophyll into the supernate. The cavitation activity did not significantly increase lipid oxidation while there was a minor trend of increased free radical production with increased ultrasound exposure. Copyright © 2013 Elsevier B.V. All rights reserved.

  14. Evaluation of conventional castaneda and lysis centrifugation blood culture techniques for diagnosis of human brucellosis.

    Science.gov (United States)

    Mantur, Basappa G; Mangalgi, Smita S

    2004-09-01

    We investigated the role of the lysis centrifugation blood culture technique over the conventional Castaneda technique for the diagnosis of human brucellosis. The lysis centrifugation technique has been found to be more sensitive in both acute (20% higher sensitivity; P centrifugation was in the mean detection time, which was only 2.4 days in acute and 2.7 days in chronic cases, with 103 out of 110 (93.6%) and 17 out of 20 (85%) cultures from acute and chronic brucellosis, respectively, detected before the conventional culture was positive. Our results confirmed the potential usefulness of the lysis technique in diagnosis and institution of appropriate antibiotic therapy.

  15. Rare incidence of tumor lysis syndrome in metastatic prostate cancer following treatment with docetaxel.

    Science.gov (United States)

    Bhardwaj, Sharonlin; Varma, Seema

    2018-03-01

    Tumor lysis syndrome is a serious and sometimes lethal complication of cancer treatment that is comprised of a set of metabolic disturbances along with clinical manifestations. Initiating chemotherapy in bulky, rapidly proliferating tumors causes rapid cell turnover that in turn releases metabolites into circulation that give rise to metabolic derangements that can be dangerous. This syndrome is usually seen in high-grade hematological malignancies. Less commonly, tumor lysis syndrome can present in solid tumors and even rarely in genitourinary tumors. In this report, the authors describe a specific case of tumor lysis syndrome in a patient with metastatic prostate cancer following treatment with docetaxel.

  16. Nucleation of holin domains and holes optimizes lysis timing of E. coli by phage λ

    Science.gov (United States)

    Ryan, Gillian; Rutenberg, Andrew

    2007-03-01

    Holin proteins regulate the precise scheduling of Escherichia coli lysis during infection by bacteriophage λ. Inserted into the host bacterium's inner membrane during infection, holins aggregate to form rafts and then holes within those rafts. We present a two-stage nucleation model of holin action, with the nucleation of condensed holin domains followed by the nucleation of holes within these domains. Late nucleation of holin rafts leads to a weak dependence of lysis timing on host cell size, though both nucleation events contribute equally to timing errors. Our simulations recover the accurate scheduling observed experimentally, and also suggest that phage-λ lysis of E.coli is optimized.

  17. Viral lysis of Phaeocystis pouchetii: implications for algal population dynamics and heterotrophic C, N and P cycling

    DEFF Research Database (Denmark)

    Haaber, Jakob Brandt Borup; Middelboe, Mathias

    2009-01-01

    in the microbial food web was associated with significant N and P mineralization, supporting the current view that viral lysates can be an important source of inorganic nutrients in marine systems. In the presence of R. salina, the generated NH(4)(+) supported 11% of the observed R. salina growth. Regrowth...... of virus-resistant P. pouchetii following cell lysis was observed in long-term incubations (150 days), and possibly influenced by nutrient availability and competition from R. salina. The observed impact of viral activity on autotrophic and heterotrophic processes provides direct experimental evidence...

  18. Catheter placement for lysis of spontaneous intracerebral hematomas: does a catheter position in the core of the hematoma allow more effective and faster hematoma lysis?

    Science.gov (United States)

    Malinova, Vesna; Schlegel, Anna; Rohde, Veit; Mielke, Dorothee

    2017-07-01

    For the fibrinolytic therapy of intracerebral hematomas (ICH) using recombinant tissue plasminogen activator (rtPA), a catheter position in the core of the hematoma along the largest clot diameter was assumed to be optimal for an effective clot lysis. However, it never had been proven that core position indeed enhances clot lysis if compared with less optimal catheter positions. In this study, the impact of the catheter position on the effectiveness and on the time course of clot lysis was evaluated. We analyzed the catheter position using a relative error calculating the distance perpendicular to the catheter's center in relation to hematoma's diameter and evaluated the relative hematoma volume reduction (RVR). The correlation of the RVR with the catheter position was evaluated. Additionally, we tried to identify patterns of clot lysis with different catheter positions. The patient's outcome at discharge was evaluated using the Glasgow outcome score. A total of 105 patients were included in the study. The mean hematoma volume was 56 ml. The overall RVR was 62.7 %. In 69 patients, a catheter position in the core of the clot was achieved. We found no significant correlation between catheter position and hematoma RVR (linear regression, p = 0.14). Core catheter position leads to more symmetrical hematoma RVR. Faster clot lysis happens in the vicinity of the catheter openings. We found no significant difference in the patient's outcome dependent on the catheter position (linear regression, p = 0.90). The catheter position in the core of the hematoma along its largest diameter does not significantly influence the effectiveness of clot lysis after rtPA application.

  19. Criticality Studies in a Pilot Plant for Processing MTR-Type Irradiated Fuels; Estudios de Criticidad de una Planta Piloto para el Tratamiento de Combustibles Irradiados Tipo ' MTR '

    Energy Technology Data Exchange (ETDEWEB)

    Pereira Sanchez, G.; Uriarte Hueda, A. [Junta de Energia Nuclear, Division de Materiales Madrid (Spain)

    1966-05-15

    A number of theoretical studies on nuclear safety have been carried out in a pilot plant being constructed at the Junta de Energia Nuclear in Madrid for processing irradiated fuels from the MTR-type experimental reactor JEN-1. The study was carried out working with aqueous and organic solutions at two levels of {sup 235}U enrichment - 20% and 93%. The paper is divided into two main parts: the first deals with the individual items of equipment, and the interactions between these are studied in the second part. The calculations in this second part have been made using three different methods to make it more certain that the system as a whole can never be critical. The first method employed is based on the solid angle concept and makes it possible to fix the maximum {sup 235}U concentrations within the system. The second method, based on the albedo, supplies the value of the multiplication factor K of the whole assembly as a function of the concentration of {sup 235}U. In the last part, the distribution of the equipment is compared with other similar systems and experimental tests from other sources. Finally, the paper specifies the conditions for working the installation which ensure that a nuclear accident can never occur. (author) [Spanish] Se ha efectuado una serie de estudios teoricos sobre la seguridad nuclear de una planta piloto, que se encuentra en construccion en la Junta de Energfa Nuclear situada en Madrid, para el tratamiento de combustibles irradiados procedentes del reactor experimental JEN-1 del tipo MTR. El estudio se ha realizado utilizando disoluciones, tanto acuosas como organicas, con dos grados de enriquecimiento, 20% y 93% en {sup 235}U. Este trabajo comprende dos partes principales: en la primera se han considerado las distintas unidades del equipo individualmente y en la segunda se han estudiado las interacciones entre ellas. El calculo de esta segunda parte se ha hecho por tres metodos diferentes para tener una mayor seguridad de que el

  20. Hypochlorite- and hypobromite-mediated radical formation and its role in cell lysis

    DEFF Research Database (Denmark)

    Hawkins, C L; Brown, B E; Davies, Michael Jonathan

    2001-01-01

    . In this study it is shown that HOBr induces red blood cell lysis at approximately 10-fold lower concentrations than HOCl, whereas with monocyte (THP1) and macrophage (J774) cells HOCl and HOBr induce lysis at similar concentrations. The role of radical formation during lysis has been investigated by EPR spin...... trapping, and it is shown that reaction of both oxidants with each cell type generates cell-derived radicals. Red blood cells exposed to nonlytic doses of HOCl generate novel nitrogen-centered radicals whose formation is GSH dependent. In contrast, HOBr gives rise to nitrogen-centered, membrane......-derived protein radicals. With lytic doses of either oxidant, protein (probably hemoglobin)-derived, nitrogen-centered radicals are observed. Unlike the red blood cells, treatment of monocytes and macrophages with HOCl gives significant radical formation only under conditions where cell lysis occurs concurrently...

  1. Mycobacterium tuberculosis bacteremia detected by the Isolator lysis-centrifugation blood culture system.

    OpenAIRE

    Kiehn, T E; Gold, J W; Brannon, P; Timberger, R J; Armstrong, D

    1985-01-01

    Mycobacterium tuberculosis was detected by the Isolator lysis-centrifugation blood culture system from the blood of a patient with tuberculosis of the breast. The organism also grew on conventional laboratory media inoculated with pleural fluid from the patient.

  2. Effects of copper, organic mercury and a mixture of the two on glycerol lysis of erythrocytes.

    OpenAIRE

    宮地,芳之

    1987-01-01

    The effects of copper, organic mercury and a mixture of the two on glycerol lysis of erythrocytes were examined. Copper ion and organic mercury (EMP; ethylmercury phosphate, and PCMB; sodium p-chloromercuricbenzoate) inhibited glycerol lysis of erythrocytes. The inhibitory effects was dependent on the incubation period. An equimolor solution of copper ion and EMP showed between copper ion and EMP. Similar results were obtained with copper and PCMB.

  3. [A comparative study of blood culture conventional method vs. a modified lysis/centrifugation technique for the diagnosis of fungemias].

    Science.gov (United States)

    Santiago, Axel Rodolfo; Hernández, Betsy; Rodríguez, Marina; Romero, Hilda

    2004-12-01

    The purpose of this work was to compare the efficacy of blood culture conventional method vs. a modified lysis/centrifugation technique. Out of 450 blood specimens received in one year, 100 where chosen for this comparative study: 60 from patients with AIDS, 15 from leukemic patients, ten from febrile neutropenic patients, five from patients with respiratory infections, five from diabetics and five from septicemic patients. The specimens were processed, simultaneously, according to the above mentioned methodologies with daily inspections searching for fungal growth in order to obtain the final identification of the causative agent. The number (40) of isolates recovered was the same using both methods, which included; 18 Candida albicans (45%), ten Candida spp. (25%), ten Histoplasma capsulatum (25%), and two Cryptococcus neoformans (5%). When the fungal growth time was compared by both methods, growth was more rapid when using the modified lysis/centrifugation technique than when using the conventional method. Statistical analysis revealed a significant difference (pcentrifugation technique showed to be more efficacious than the conventional one, and therefore the implementation of this methodology is highly recommended for the isolation of fungi from blood.

  4. Radiation-induced enhancement of enzymatic cell lysis of Micrococcus radiodurans

    International Nuclear Information System (INIS)

    Watanabe, H.; Takehisa, M.; Iizuka, H.

    1981-01-01

    The intact cells of M. radiodurans were rendered sensitive to the action of lytic enzyme (P2-2 enzyme) by irradiation. The radiation-induced enhancement of cell lysis with P2-2 enzyme was completely prevented by the addition of t-butanol and irradiation at liquid nitrogen temperature. These results indicate that the enhancement is due to indirect action resulting from OH radicals. Cell lysis by lysozyme was enhanced only when the cells were irradiated under N 2 O. The enhancement of cell lysis with lysozyme was also prevented by adding alcohols. On the other hand, when lipid components in cells were removed by extraction with n-butanol, the radiation-induced enhancement of cell lysis with P2-2 enzyme and lysozyme was not observed. From these results it is concluded that the enhancement of enzymatic cell lysis by irradiation is attributable to alteration in the lipid-rich layer of the cell wall caused by OH radicals

  5. Radiation-induced enhancement of enzymatic cell lysis of Micrococcus radiodurans

    Energy Technology Data Exchange (ETDEWEB)

    Watanabe, H.; Takehisa, M. [Japan Atomic Energy Research Inst., Takasaki, Gunma, Takasaki Radiation Chemistry Research Establishment (Japan); Iizuka, H.

    1981-10-15

    The intact cells of M. radiodurans were rendered sensitive to the action of lytic enzyme (P2-2 enzyme) by irradiation. The radiation-induced enhancement of cell lysis with P2-2 enzyme was completely prevented by the addition of t-butanol and irradiation at liquid nitrogen temperature. These results indicate that the enhancement is due to indirect action resulting from OH radicals. Cell lysis by lysozyme was enhanced only when the cells were irradiated under N{sub 2}O. The enhancement of cell lysis with lysozyme was also prevented by adding alcohols. On the other hand, when lipid components in cells were removed by extraction with n-butanol, the radiation-induced enhancement of cell lysis with P2-2 enzyme and lysozyme was not observed. From these results it is concluded that the enhancement of enzymatic cell lysis by irradiation is attributable to alteration in the lipid-rich layer of the cell wall caused by OH radicals.

  6. [Treatment of surface burns with proteolytic enzymes: mathematic description of lysis kinetics].

    Science.gov (United States)

    Domogatskaia, A S; Domogatskiĭ, S P; Ruuge, E K

    2003-01-01

    The lysis of necrotic tissue by a proteolytic enzyme applied to the surface of a burn wound was studied. A mathematical model was proposed, which describes changes in the thickness of necrotic tissue as a function of the proteolytic activity of the enzyme. The model takes into account the inward-directed diffusion of the enzyme, the counterflow of interstitial fluid (exudates) containing specific inhibitors, and the extracellular matrix proteolysis. It was shown in terms of the quasi-stationary approach that the thickness of the necrotic tissue layer decreases exponentially with time; i.e., the lysis slows down as the thickness of the necrotic tissue layer decreases. The dependence of the characteristic time of this decrease on enzyme concentration was obtained. It was shown that, at high enzyme concentrations (more than 5 mg/ml), the entire time of lysis (after the establishment of quasi-stationary equilibrium) is inversely proportional to the concentration of the enzyme.

  7. Lysis-deficient phages as novel therapeutic agents for controlling bacterial infection

    Directory of Open Access Journals (Sweden)

    Kempashanaiah Nanjundappa

    2011-08-01

    Full Text Available Abstract Background Interest in phage therapy has grown over the past decade due to the rapid emergence of antibiotic resistance in bacterial pathogens. However, the use of bacteriophages for therapeutic purposes has raised concerns over the potential for immune response, rapid toxin release by the lytic action of phages, and difficulty in dose determination in clinical situations. A phage that kills the target cell but is incapable of host cell lysis would alleviate these concerns without compromising efficacy. Results We developed a recombinant lysis-deficient Staphylococcus aureus phage P954, in which the endolysin gene was rendered nonfunctional by insertional inactivation. P954, a temperate phage, was lysogenized in S. aureus strain RN4220. The native endolysin gene on the prophage was replaced with an endolysin gene disrupted by the chloramphenicol acetyl transferase (cat gene through homologous recombination using a plasmid construct. Lysogens carrying the recombinant phage were detected by growth in presence of chloramphenicol. Induction of the recombinant prophage did not result in host cell lysis, and the phage progeny were released by cell lysis with glass beads. The recombinant phage retained the endolysin-deficient genotype and formed plaques only when endolysin was supplemented. The host range of the recombinant phage was the same as that of the parent phage. To test the in vivo efficacy of the recombinant endolysin-deficient phage, immunocompromised mice were challenged with pathogenic S. aureus at a dose that results in 80% mortality (LD80. Treatment with the endolysin-deficient phage rescued mice from the fatal S. aureus infection. Conclusions A recombinant endolysin-deficient staphylococcal phage has been developed that is lethal to methicillin-resistant S. aureus without causing bacterial cell lysis. The phage was able to multiply in lytic mode utilizing a heterologous endolysin expressed from a plasmid in the propagation host

  8. Tumor lysis syndrome following endoscopic radiofrequency interstitial thermal ablation of colorectal liver metastases.

    LENUS (Irish Health Repository)

    Barry, B D

    2012-02-03

    Radiofrequency interstitial thermal ablation (RITA) provides a palliative option for patients suffering from metastatic liver disease. This procedure can be performed using a laparoscopic approach with laparoscopic ultrasound used to position the RITA probe. We describe a case of laparoscopic RITA performed for colorectal liver metastasis that was complicated by tumor lysis syndrome (TLS) following treatment. We consider RITA to be a safe procedure, as supported by the literature, but where intracorporal tumor lysis is the treatment goal we believe that the systemic release of tumor products can overwhelm the excretory capacity; therefore, TLS is an inevitable consequence in some patients.

  9. Complement lysis activity in autologous plasma is associated with lower viral loads during the acute phase of HIV-1 infection.

    Directory of Open Access Journals (Sweden)

    Michael Huber

    2006-11-01

    Full Text Available BACKGROUND: To explore the possibility that antibody-mediated complement lysis contributes to viremia control in HIV-1 infection, we measured the activity of patient plasma in mediating complement lysis of autologous primary virus. METHODS AND FINDINGS: Sera from two groups of patients-25 with acute HIV-1 infection and 31 with chronic infection-were used in this study. We developed a novel real-time PCR-based assay strategy that allows reliable and sensitive quantification of virus lysis by complement. Plasma derived at the time of virus isolation induced complement lysis of the autologous virus isolate in the majority of patients. Overall lysis activity against the autologous virus and the heterologous primary virus strain JR-FL was higher at chronic disease stages than during the acute phase. Most strikingly, we found that plasma virus load levels during the acute but not the chronic infection phase correlated inversely with the autologous complement lysis activity. Antibody reactivity to the envelope (Env proteins gp120 and gp41 were positively correlated with the lysis activity against JR-FL, indicating that anti-Env responses mediated complement lysis. Neutralization and complement lysis activity against autologous viruses were not associated, suggesting that complement lysis is predominantly caused by non-neutralizing antibodies. CONCLUSIONS: Collectively our data provide evidence that antibody-mediated complement virion lysis develops rapidly and is effective early in the course of infection; thus it should be considered a parameter that, in concert with other immune functions, steers viremia control in vivo.

  10. Mechanisms of fever production and lysis: lessons from experimental LPS fever.

    Science.gov (United States)

    Roth, Joachim; Blatteis, Clark M

    2014-10-01

    Fever is a cardinal symptom of infectious or inflammatory insults, but it can also arise from noninfectious causes. The fever-inducing agent that has been used most frequently in experimental studies designed to characterize the physiological, immunological and neuroendocrine processes and to identify the neuronal circuits that underlie the manifestation of the febrile response is lipopolysaccharide (LPS). Our knowledge of the mechanisms of fever production and lysis is largely based on this model. Fever is usually initiated in the periphery of the challenged host by the immediate activation of the innate immune system by LPS, specifically of the complement (C) cascade and Toll-like receptors. The first results in the immediate generation of the C component C5a and the subsequent rapid production of prostaglandin E2 (PGE2). The second, occurring after some delay, induces the further production of PGE2 by induction of its synthesizing enzymes and transcription and translation of proinflammatory cytokines. The Kupffer cells (Kc) of the liver seem to be essential for these initial processes. The subsequent transfer of the pyrogenic message from the periphery to the brain is achieved by neuronal and humoral mechanisms. These pathways subserve the genesis of early (neuronal signals) and late (humoral signals) phases of the characteristically biphasic febrile response to LPS. During the course of fever, counterinflammatory factors, "endogenous antipyretics," are elaborated peripherally and centrally to limit fever in strength and duration. The multiple interacting pro- and antipyretic signals and their mechanistic effects that underlie endotoxic fever are the subjects of this review.

  11. Clot lysis time in platelet-rich plasma: method assessment, comparison with assays in platelet-free and platelet-poor plasmas, and response to tranexamic acid.

    Science.gov (United States)

    Panes, Olga; Padilla, Oslando; Matus, Valeria; Sáez, Claudia G; Berkovits, Alejandro; Pereira, Jaime; Mezzano, Diego

    2012-01-01

    Fibrinolysis dysfunctions cause bleeding or predisposition to thrombosis. Platelets contain several factors of the fibrinolytic system, which could up or down regulate this process. However, the temporal relationship and relative contributions of plasma and platelet components in clot lysis are mostly unknown. We developed a clot lysis time (CLT) assay in platelet-rich plasma (PRP-CLT, with and without stimulation) and compared it to a similar one in platelet-free plasma (PFP) and to another previously reported test in platelet-poor plasma (PPP). We also studied the differential effects of a single dose of tranexamic acid (TXA) on these tests in healthy subjects. PFP- and PPP-CLT were significantly shorter than PRP-CLT, and the three assays were highly correlated (p plasma PAI-1, von Willebrand factor, fibrinogen, LDL-cholesterol, and triglycerides (p platelet aggregation/secretion, platelet counts, and pro-coagulant tests to explore factor X activation by platelets, PRP clotting time, and thrombin generation in PRP. Among all the studied variables, PFP-CLT was independently associated with plasma PAI-1, LDL-cholesterol, and triglycerides and, additionally, stimulated PRP-CLT was also independently associated with plasma fibrinogen. A single 1 g dose of TXA strikingly prolonged all three CLTs, but in contrast to the results without the drug, the lysis times were substantially shorter in non-stimulated or stimulated PRP than in PFP and PPP. This standardized PRP-CLT may become a useful tool to study the role of platelets in clot resistance and lysis. Our results suggest that initially, the platelets enmeshed in the clot slow down the fibrinolysis process. However, the increased clot resistance to lysis induced by TXA is overcome earlier in platelet-rich clots than in PFP or PPP clots. This is likely explained by the display of platelet pro-fibrinolytic effects. Focused research is needed to disclose the mechanisms for the relationship between CLT and plasma

  12. Plasma Clot Lysis Time and Its Association with Cardiovascular Risk Factors in Black Africans

    NARCIS (Netherlands)

    Z. de Lange (Zelda); M. Pieters (Marlien); J.C. Jerling (Johann); A. Kruger (Annamarie); D.C. Rijken (Dingeman)

    2012-01-01

    textabstractStudies in populations of European descent show longer plasma clot lysis times (CLT) in patients with cardiovascular disease (CVD) than in controls. No data are available on the association between CVD risk factors and fibrinolytic potential in black Africans, a group undergoing rapid

  13. Changes in bacterial and eukaryotic community structure after mass lysis of filamentous cyanobacteria associated with viruses

    NARCIS (Netherlands)

    Hannen, E.J. van; Zwart, G.; Agterveld, M.P. van; Gons, H.J.; Ebert, J.; Laanbroek, H.J.

    1999-01-01

    During an experiment in two laboratory-scale enclosures filled with lake water (130 liters each) we noticed the almost-complete lysis of the cyanobacterial population. Based on electron microscopic observations of viral particles inside cyanobacterial filaments and counts of virus-like particles,

  14. Changes in bacterial and eukaryotic community structure after mass lysis of filamentous cyanobacteria associated with viruses

    NARCIS (Netherlands)

    Van Hannen, E.J.; Zwart, G.; Van Agterveld, M.P.; Gons, H.J.; Ebert, J.; Laanbroek, H.J.

    1999-01-01

    During an experiment in two laboratory-scale enclosures filled with lake water (130 liters each) we noticed the almost-complete lysis of the cyanobacterial population. Based on electron microscopic observations of viral particles inside cyanobacterial filaments and counts of virus-like particles, we

  15. Quantifying enzymatic lysis: estimating the combined effects of chemistry, physiology and physics

    International Nuclear Information System (INIS)

    Mitchell, Gabriel J; Weitz, Joshua S; Nelson, Daniel C

    2010-01-01

    The number of microbial pathogens resistant to antibiotics continues to increase even as the rate of discovery and approval of new antibiotic therapeutics steadily decreases. Many researchers have begun to investigate the therapeutic potential of naturally occurring lytic enzymes as an alternative to traditional antibiotics. However, direct characterization of lytic enzymes using techniques based on synthetic substrates is often difficult because lytic enzymes bind to the complex superstructure of intact cell walls. Here we present a new standard for the analysis of lytic enzymes based on turbidity assays which allow us to probe the dynamics of lysis without preparing a synthetic substrate. The challenge in the analysis of these assays is to infer the microscopic details of lysis from macroscopic turbidity data. We propose a model of enzymatic lysis that integrates the chemistry responsible for bond cleavage with the physical mechanisms leading to cell wall failure. We then present a solution to an inverse problem in which we estimate reaction rate constants and the heterogeneous susceptibility to lysis among target cells. We validate our model given simulated and experimental turbidity assays. The ability to estimate reaction rate constants for lytic enzymes will facilitate their biochemical characterization and development as antimicrobial therapeutics

  16. A simple and novel modification of comet assay for determination of bacteriophage mediated bacterial cell lysis.

    Science.gov (United States)

    Khairnar, Krishna; Sanmukh, Swapnil; Chandekar, Rajshree; Paunikar, Waman

    2014-07-01

    The comet assay is the widely used method for in vitro toxicity testing which is also an alternative to the use of animal models for in vivo testing. Since, its inception in 1984 by Ostling and Johansson, it is being modified frequently for a wide range of application. In spite of its wide applicability, unfortunately there is no report of its application in bacteriophages research. In this study, a novel application of comet assay for the detection of bacteriophage mediated bacterial cell lysis was described. The conventional methods in bacteriophage research for studying bacterial lysis by bacteriophages are plaque assay method. It is time consuming, laborious and costly. The lytic activity of bacteriophage devours the bacterial cell which results in the release of bacterial genomic material that gets detected by ethidium bromide staining method by the comet assay protocol. The objective of this study was to compare efficacy of comet assay with different assay used to study phage mediated bacterial lysis. The assay was performed on culture isolates (N=80 studies), modified comet assay appear to have relatively higher sensitivity and specificity than other assay. The results of the study showed that the application of comet assay can be an economical, time saving and less laborious alternative to conventional plaque assay for the detection of bacteriophage mediated bacterial cell lysis. Copyright © 2014 Elsevier B.V. All rights reserved.

  17. Lysis of fresh human solid tumors by autologous lymphocytes activated in vitro with lectins

    International Nuclear Information System (INIS)

    Mazumder, A.; Grimm, E.A.; Zhang, H.Z.; Rosenberg, S.A.

    1982-01-01

    Human peripheral blood lymphocytes (PBL), obtained from patients with a variety of cancers, were incubated in vitro with phytohemagglutinin, concanavalin A, and crude or lectin-free T-cell growth factors. The lectin-activated PBL of nine patients were capable of lysing fresh autologous tumor during a 4-hr 51Cr release assay. Multiple metastases from the same patient were equivalently lysed by these activated autologous PBL. No lysis of fresh PBL or lectin-induced lymphoblast cell targets was seen, although tumor, PBL, and lymphoblast cells were shown to be equally lysable using allosensitized cells. The activated cells could be expanded without loss of cytotoxicity in crude or lectin-free T-cell growth factors. The generation of cells lytic to fresh autologous tumor was dependent on the presence of adherent cells, although the lytic cell itself was not adherent. Proliferation was not involved in the induction of lytic cells since equal lysis was induced in irradiated and nonirradiated lymphocytes. Lectin was not required in the lytic assay, and the addition of alpha-methyl-D-mannoside to concanavalin A-activated lymphoid cells did not increase the lysis of fresh tumor cells. Activation by lectin for 3 days appears to be an efficient and convenient method for generating human cells lytic to fresh autologous tumor. These lytic cells may be of value for studies of the cell-mediated lysis of human tumor and possibly for tumor immunotherapy as well

  18. Quantifying enzymatic lysis: estimating the combined effects of chemistry, physiology and physics.

    Science.gov (United States)

    Mitchell, Gabriel J; Nelson, Daniel C; Weitz, Joshua S

    2010-10-04

    The number of microbial pathogens resistant to antibiotics continues to increase even as the rate of discovery and approval of new antibiotic therapeutics steadily decreases. Many researchers have begun to investigate the therapeutic potential of naturally occurring lytic enzymes as an alternative to traditional antibiotics. However, direct characterization of lytic enzymes using techniques based on synthetic substrates is often difficult because lytic enzymes bind to the complex superstructure of intact cell walls. Here we present a new standard for the analysis of lytic enzymes based on turbidity assays which allow us to probe the dynamics of lysis without preparing a synthetic substrate. The challenge in the analysis of these assays is to infer the microscopic details of lysis from macroscopic turbidity data. We propose a model of enzymatic lysis that integrates the chemistry responsible for bond cleavage with the physical mechanisms leading to cell wall failure. We then present a solution to an inverse problem in which we estimate reaction rate constants and the heterogeneous susceptibility to lysis among target cells. We validate our model given simulated and experimental turbidity assays. The ability to estimate reaction rate constants for lytic enzymes will facilitate their biochemical characterization and development as antimicrobial therapeutics.

  19. Lysis of typhus-group rickettsia-infected targets by lymphokine activated killers

    International Nuclear Information System (INIS)

    Carl, M.; Dasch, G.A.

    1986-01-01

    The authors recently described a subset of OKT8, OKT3-positive lymphocytes from typhus-group rickettsia immune individuals which were capable of lysing autologous PHA-blasts or Epstein-Barr virus transformed B cells (LCL) infected with typhus-group rickettsiae. In order to determine if killing by these effectors was HLA-restricted, they stimulated peripheral blood mononuclear cells (PBMC) from typhus-group rickettsia immune individuals in vitro with typhus-group rickettsia-derived antigen for one week and then measured lysis of autologous LCL or HLA-mismatched LCL in a 4-6 hour Cr 51 -release assay. There was significant lysis of both the autologous and the HLA-mismatched infected targets as compared to the corresponding uninfected targets. Since this suggested that the effectors were lymphokine activated killers (LAK) rather than cytotoxic T lymphocytes, they then tested this hypothesis by stimulating PBMC from both immune and non-immune individuals in vitro for one week with purified interleukin 2 and measuring lysis of infected, autologous LCL. PBMC thus treated, from both immune and non-immune individuals, were capable of significantly lysing autologous, infected LCL as compared to the non-infected control. They therefore conclude that targets infected with typhus-group rickettsiae are susceptible to lysis to LAK

  20. Electrochemical lysis at the stage of endoresection for large posterior intraocular tumors

    Directory of Open Access Journals (Sweden)

    Yu. A. Belyy

    2012-01-01

    Full Text Available Purpose: to design the new combined technique of endoresection with intraoperative intraocular electrochemical lysis at the tumor destruction stage for large posterior intraocular tumors.Methods: 3 patients (3 eyes with large choroidal melanomas t3N0M0 (tumor thickness — 8-10 mm, base diameter — 13-15 mm, juxtapapillary localization. Mean age was 55.4 years old. Endoresection with intraoperational intraocular electrochemical lysis of the tumor was performed. Electrochemical lysis was performed with use of the technical unit ECU 300 (Soering, Germany and the original method of combined intratumoral positioning of two platinum electrodes: anode and cathode.Results: the tumor was removal completely in all 3 cases. the anatomical retinal reattachment was reached in all patients. Sclera was safe in all 3 cases. Visual acuity was not changed (NLP. At the place of the removal tumor a surgical choroidal coloboma without pigmentation all over scleral bed and periphery was shown in all cases in distant postoperative period (from 1.5 to 3 years. No local recurrences or metastasis were revealed in all patients.Conclusion: Further investigations in clinical group are necessarily to determinate the real possibilities of the combined method and the indications for endoresection with intraoperative intraocular electrochemical lysis for large intraocular tumors. 

  1. Fractalkine expression induces endothelial progenitor cell lysis by natural killer cells.

    Directory of Open Access Journals (Sweden)

    Dilyana Todorova

    Full Text Available BACKGROUND: Circulating CD34(+ cells, a population that includes endothelial progenitors, participate in the maintenance of endothelial integrity. Better understanding of the mechanisms that regulate their survival is crucial to improve their regenerative activity in cardiovascular and renal diseases. Chemokine-receptor cross talk is critical in regulating cell homeostasis. We hypothesized that cell surface expression of the chemokine fractalkine (FKN could target progenitor cell injury by Natural Killer (NK cells, thereby limiting their availability for vascular repair. METHODOLOGY/PRINCIPAL FINDINGS: We show that CD34(+-derived Endothelial Colony Forming Cells (ECFC can express FKN in response to TNF-α and IFN-γ inflammatory cytokines and that FKN expression by ECFC stimulates NK cell adhesion, NK cell-mediated ECFC lysis and microparticles release in vitro. The specific involvement of membrane FKN in these processes was demonstrated using FKN-transfected ECFC and anti-FKN blocking antibody. FKN expression was also evidenced on circulating CD34(+ progenitor cells and was detected at higher frequency in kidney transplant recipients, when compared to healthy controls. The proportion of CD34(+ cells expressing FKN was identified as an independent variable inversely correlated to CD34(+ progenitor cell count. We further showed that treatment of CD34(+ circulating cells isolated from adult blood donors with transplant serum or TNF-α/IFN-γ can induce FKN expression. CONCLUSIONS: Our data highlights a novel mechanism by which FKN expression on CD34(+ progenitor cells may target their NK cell mediated killing and participate to their immune depletion in transplant recipients. Considering the numerous diseased contexts shown to promote FKN expression, our data identify FKN as a hallmark of altered progenitor cell homeostasis with potential implications in better evaluation of vascular repair in patients.

  2. A mechanism of acquired resistance to complement-mediated lysis by Entamoeba histolytica.

    Science.gov (United States)

    Gutiérrez-Kobeh, L; Cabrera, N; Pérez-Montfort, R

    1997-04-01

    Some Entamoeba histolytica strains resist complement-mediated lysis by serum. Susceptible and resistant strains activate the complement system equivalently, but resistant amebas evade killing by membrane attack complexes. Our objective was to determine the mechanism by which trophozoites of E. histolytica resist lysis by human serum. Amebas were made resistant to lysis by incubation with increasing concentrations of normal human serum. The possibility that resistant cells ingest membrane attack complexes was explored by subcellular fractionation of susceptible and resistant trophozoites treated with sublytic concentrations of human serum containing radiolabeled C9. In both cases, most of the label was in the fractions containing plasma membrane. The susceptible strain consistently showed more label associated with these fractions than the resistant strain. Thus, the possibility that the membrane attack complexes were released to the medium was explored. Both resistant and susceptible trophozoites release to the medium similar amounts of material excluded by Sepharose CL-2B in the presence or absence of normal human serum. Labeled C9 elutes together with the main bulk of proteins from the medium: this indicates that it is not in vesicles or high molecular weight aggregates. Coincubation of susceptible amebas with lysates of resistant trophozoites confers resistance to susceptible cells within 30 min. Resistance to lysis by serum can also be acquired by susceptible amebas after coincubation with lysates from human erythrocytes or after feeding them with whole human red blood cells. Resistant but not susceptible trophozoites show intense immunofluorescent staining on their surface with anti-human erythrocytic membrane antibody. These results suggest that amebas acquire resistance to lysis by serum by incorporating into their membranes complement regulatory proteins.

  3. Method and apparatus for iterative lysis and extraction of algae

    Science.gov (United States)

    Chew, Geoffrey; Boggs, Tabitha; Dykes, Jr., H. Waite H.; Doherty, Stephen J.

    2015-12-01

    A method and system for processing algae involves the use of an ionic liquid-containing clarified cell lysate to lyse algae cells. The resulting crude cell lysate may be clarified and subsequently used to lyse algae cells. The process may be repeated a number of times before a clarified lysate is separated into lipid and aqueous phases for further processing and/or purification of desired products.

  4. Propuesta sobre el proceso productivo para financiar empresas del deporte a partir del estudio de sus seguidores = Proposal on the production process to finance companies sport from studying his followers

    OpenAIRE

    Cárdenas-Polanía, Wilson Andrés

    2015-01-01

    Cárdenas-Polanía Wilson Andrés. Propuesta sobre el proceso productivo para financiar empresas del deporte a partir del estudio de sus seguidores = Proposal on the production process to finance companies sport from studying his followers. Journal of Education, Health and Sport. 2015;5(12):630-646. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.44759 http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%2812%29%3A630-646 http://pbn.nauka.gov.pl/works/694742 Formerly Jour...

  5. de estudios observacionales

    Directory of Open Access Journals (Sweden)

    Erik von Elm

    2008-01-01

    un documento de explicación y elaboración al que puede accederse libremente en los sitios web de PLoS Medicine, Annals of Internal Medicine y Epidemiology. Esperamos que la declaración STROBE contribuya a mejorar la calidad de la publicación de los estudios observacionales.

  6. Superiority of SDS lysis over saponin lysis for direct bacterial identification from positive blood culture bottle by MALDI-TOF MS.

    Science.gov (United States)

    Caspar, Yvan; Garnaud, Cécile; Raykova, Mariya; Bailly, Sébastien; Bidart, Marie; Maubon, Danièle

    2017-05-01

    Fast species diagnosis has an important health care impact, as rapid and specific antibacterial therapy is of clear benefit for patient's outcome. Here, a new protocol for species identification directly from positive blood cultures is proposed. Four in-house protocols for bacterial identification by MS directly from clinical positive blood cultures evaluating two lytic agents, SDS and saponin, and two protein extraction schemes, fast (FP) and long (LP) are compared. One hundred and sixty-eight identification tests are carried out on 42 strains. Overall, there are correct identifications to the species level in 90% samples for the SDS-LP, 60% for the SDS-FP, 48% for the saponin LP, and 43% for the saponin FP. Adapted scores allowed 92, 86, 72, and 53% identification for SDS-LP, SDS-FP, saponin LP, and saponin FP, respectively. Saponin lysis is associated with a significantly lower score compared to SDS (0.87 [0.83-0.92], p-value saponin lysis and the application of this rapid and cost-effective protocol in daily routine for microbiological agents implicated in septicemia. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  7. Spontaneous tumour lysis syndrome in a case of multiple myeloma – A rare occurrence

    Directory of Open Access Journals (Sweden)

    Kavitha Saravu

    2013-03-01

    Full Text Available We describe a case of a 40-year-old male patient who was found to have multiple myeloma with spontaneous tumour lysis syndrome (TLS, following a compression fracture of the L–2 vertebrae. Multiple myeloma was confirmed by bone marrow analysis and the M–band on serum protein electrophoresis. Hyperuricaemia (26.2 mg/dL, hyperkalaemia (> 7.0 mEq/L, hyperphosphatemia (16.2 mg of phosphorus/dL, normocalcemia and acute kidney injury, prior to anticancer treatment suggested spontaneous TLS. Inciting events for tumour lysis, such as chemotherapy, dehydration and exposure to steroids were absent. Patient received hydration, hypourecemic drugs and haemodialysis. This case report highlights the rare presentation of multiple myeloma with spontaneous TLS.

  8. A case of cetuximab-related tumour lysis syndrome in metastatic rectal carcinoma

    Science.gov (United States)

    Haroon, Muhammad; Kwong, Whye Yan; Cantwell, Brian; Walker, Frank

    2010-01-01

    A 60-year-old man was diagnosed with a moderately differentiated adenocarcinoma in November 2006. The computed tomography (CT), magnetic resonance imaging (MRI) and whole-body positron emission tomography–CT (PET–CT) scan showed the presence of multiple liver metastases which were confined to its right lobe. He had the first session of a combined therapy with cetuximab and 5-fluorouracil (5-FU) in March 2009; however, soon afterwards, he presented with the symptoms, signs and biochemistry suggestive of tumour lysis syndrome. Our unusual case highlights that tumour lysis syndrome can also develop in ‘low risk’ category tumours, and that clinicians should be vigilant in identifying at-risk patients. PMID:28657052

  9. Mass entrapment and lysis of Mesodinium rubrum cells in mucus threads observed in cultures with Dinophysis

    DEFF Research Database (Denmark)

    Ojamäe, Karin; Hansen, Per Juel; Lips, Inga

    2016-01-01

    The entrapment and death of the ciliate Mesodinium rubrum in the mucus threads in cultures with Dinophysis is described and quantified. Feeding experiments with different concentrations and predator–prey ratios of Dinophysis acuta, Dinophysis acuminata and M. rubrum to study the motility loss...... and aggregate formation of the ciliates and the feeding behaviour of Dinophysis were carried out. In cultures of either Dinophysis species, the ciliates became entrapped in the mucus, which led to the formation of immobile aggregates of M. rubrum and subsequent cell lysis. The proportion of entrapped ciliates...... was influenced by the concentration of Dinophysis and the ratio of predator and prey in the cultures. At high cell concentrations of prey (136 cells mL−1) and predator (100 cells mL−1), a maximum of 17% of M. rubrum cells became immobile and went through cell lysis. Ciliates were observed trapped in the mucus...

  10. Cell-density-dependent lysis and sporulation of Myxococcus xanthus in agarose microbeads.

    OpenAIRE

    Rosenbluh, A; Nir, R; Sahar, E; Rosenberg, E

    1989-01-01

    Vegetative cells of Myxococcus xanthus were immobilized in 25-microns-diameter agarose microbeads and incubated in either growth medium or sporulation buffer. In growth medium, the cells multiplied, glided to the periphery, and then filled the beads. In sporulation buffer, up to 90% of the cells lysed and ca. 50% of the surviving cells formed resistant spores. A strong correlation between sporulation and cell lysis was observed; both phenomena were cell density dependent. Sporulation proficie...

  11. [Intraoperative lysis and neurostimulation as added therapy in surgery of popliteal artery aneurysm].

    Science.gov (United States)

    Peiper, C; Heye, K; Ktenidis, K; Horsch, S

    1997-01-01

    Additional therapy of symptomatic popliteal artery aneurysm includes intraoperative lysis for the treatment of the descending thrombosis and spinal cord stimulation for cases of chronic deterioration of the peripheral perfusion state. Between 1989 and 1996, we treated 50 patients with 55 symptomatic aneurysms using this concept, 18 of them as emergency cases. We reached a postoperative amputation rate of 12.7% and good long-term functional results in 34 of 37 patients.

  12. Arthroscopic lysis and lavage in patients with temporomandibular anterior disc displacement without reduction

    Czech Academy of Sciences Publication Activity Database

    Machoň, V.; Šedý, Jiří; Klíma, K.; Hirjak, D.; Foltán, R.

    2012-01-01

    Roč. 41, č. 1 (2012), s. 109-113 ISSN 0901-5027 R&D Projects: GA MŠk(CZ) LC554; GA ČR GAP304/10/0320 Grant - others:GA MŠk(CZ) 1M0538 Program:1M Institutional research plan: CEZ:AV0Z50390703 Keywords : temporomandibular joint * arthroscopic lysis * arthroscopic lavage Subject RIV: FJ - Surgery incl. Transplants Impact factor: 1.521, year: 2012

  13. Normal human serum (HS) prevents oxidant-induced lysis of cultured endothelial cells (ECs)

    International Nuclear Information System (INIS)

    Callahan, K.S.; Harlan, J.M.

    1986-01-01

    Most studies demonstrating oxidant lysis of cultured ECs are performed in serum-free media or media containing low concentrations of bovine serum. The authors found that HS protects human and bovine ECs from lysis caused by reagent H 2 O 2 or glucose/glucose oxidase (GO)-generated H 2 O 2 . EC injury was assessed by 51 Cr release, cell detachment, or trypan blue dye exclusion. Protective HS activity was dose-dependent with concentrations greater than or equal to 25% preventing lethal injury. Cytotoxicity at 24 hrs, induced by 20 mU/ml GO, was 90.1 +/- 5.2% without HS vs 1.7 +/- 4.6% with 25% HS present (20 exp). Similar protection was observed with heparinized plasma. Of note, comparable concentrations of bovine serum were devoid of protective activity. Addition of fatty acid-free albumin to the media was also without protective effect. Preliminary characterization showed HS activity was stable to 60 0 C for 30 min, non-dialyzable at 25,000 MW cutoff, and retained in delipidated serum. The HS protection was not merely due to scavenging of exogenous H 2 O 2 as A23187-induced EC lysis was also prevented by HS. Protective activity was not reproduced by purified cerruloplasmin or transferrin. In conclusion, unidentified factor(s) present in HS protect cultured ECs from oxidant-induced lysis. Since endothelium is normally exposed to 100% plasma, the authors suggest that in vitro studies of oxidant-mediated injury be performed in the presence of HS. Factor(s) in HS may play an important role in modulating oxidant-induced vascular injury in vivo

  14. Design and Modelling of a Microfluidic Electro-Lysis Device with Controlling Plates

    Science.gov (United States)

    Jenkins, A.; Chen, C. P.; Spearing, S.; Monaco, L. A.; Steele, A.; Flores, G.

    2006-04-01

    Many Lab-on-Chip applications require sample pre-treatment systems. Using electric fields to perform cell lysis in bio-MEMS systems has provided a powerful tool which can be integrated into Lab-on-a- Chip platforms. The major design considerations for electro-lysis devices include optimal geometry and placement of micro-electrodes, cell concentration, flow rates, optimal electric field (e.g. pulsed DC vs. AC), etc. To avoid electrolysis of the flowing solution at the exposed electrode surfaces, magnitudes and the applied voltages and duration of the DC pulse, or the AC frequency of the AC, have to be optimized for a given configuration. Using simulation tools for calculation of electric fields has proved very useful, for exploring alternative configurations and operating conditions for achieving electro cell-lysis. To alleviate the problem associated with low electric fields within the microfluidics channel and the high voltage demand on the contact electrode strips, two ''control plates'' are added to the microfluidics configuration. The principle of placing the two controlling plate-electrodes is based on the electric fields generated by a combined insulator/dielectric (glass/water) media. Surface charges are established at the insulator/dielectric interface. This paper discusses the effects of this interface charge on the modification of the electric field of the flowing liquid/cell solution.

  15. Comparison of the lysis-centrifugation and agitated biphasic blood culture systems for detection of fungemia.

    Science.gov (United States)

    Murray, P R

    1991-01-01

    Although the detection of fungemia has been improved by the use of vented or biphasic blood culture bottles, the best recovery and earliest detection have been reported in the Isolator lysis-centrifugation system. It was recently demonstrated that improved detection of both bacteria and fungi was accomplished by mechanically agitating blood culture bottles for the first 24 h of incubation. In this study the detection of fungemia by use of the Isolator system was compared with that of an agitated biphasic system. A total of 182 fungi were isolated from blood specimens inoculated into both culture systems. No difference in the overall recovery of fungi or individual species of yeasts was observed between the two systems. However, all seven isolates of Histoplasma capsulatum were recovered in the Isolator system only. The time required to detect fungemia with each of the two systems was also compared. No statistically significant difference was observed. From the data collected during this 18-month study, it can be concluded that the overall recovery and time of detection of yeasts are equivalent in the lysis-centrifugation system and the agitated biphasic blood culture system. The lysis-centrifugation system is still superior for the detection of filamentous fungi such as H. capsulatum. PMID:1993772

  16. A self-lysis pathway that enhances the virulence of a pathogenic bacterium.

    Science.gov (United States)

    McFarland, Kirsty A; Dolben, Emily L; LeRoux, Michele; Kambara, Tracy K; Ramsey, Kathryn M; Kirkpatrick, Robin L; Mougous, Joseph D; Hogan, Deborah A; Dove, Simon L

    2015-07-07

    In mammalian cells, programmed cell death (PCD) plays important roles in development, in the removal of damaged cells, and in fighting bacterial infections. Although widespread among multicellular organisms, there are relatively few documented instances of PCD in bacteria. Here we describe a potential PCD pathway in Pseudomonas aeruginosa that enhances the ability of the bacterium to cause disease in a lung infection model. Activation of the system can occur in a subset of cells in response to DNA damage through cleavage of an essential transcription regulator we call AlpR. Cleavage of AlpR triggers a cell lysis program through de-repression of the alpA gene, which encodes a positive regulator that activates expression of the alpBCDE lysis cassette. Although this is lethal to the individual cell in which it occurs, we find it benefits the population as a whole during infection of a mammalian host. Thus, host and pathogen each may use PCD as a survival-promoting strategy. We suggest that activation of the Alp cell lysis pathway is a disease-enhancing response to bacterial DNA damage inflicted by the host immune system.

  17. Effect of ultrasonic frequency on the mechanism of formic acid sono-lysis

    International Nuclear Information System (INIS)

    Chave, T.; Nikitenko, S.I.; Navarro, N.M.; Pochon, P.; Bisel, I.

    2011-01-01

    The kinetics and mechanism of formic acid sono-chemical degradation were studied at ultrasonic frequencies of 20, 200, and 607 kHz under argon atmosphere. Total yield of HCOOH sono-chemical degradation increases approximately 6-8-fold when the frequency increased from 20 to 200 or to 607 kHz. At low ultrasonic frequencies, HCOOH degradation has been attributed to oxidation with OH . radicals from water sono-lysis and to the HCOOH decarboxylation occurring at the cavitation bubble-liquid interface. With high-frequency ultrasound, the sono-chemical reaction is also influenced by HCOOH dehydration. Whatever the ultrasonic frequency, the sono-lysis of HCOOH yielded H 2 and CO 2 in the gas phase as well as trace, amounts of oxalic acid and formaldehyde in the liquid phase. However, CO and CH 4 formations were only detected under high frequency ultrasound. The most striking difference between low frequency and high frequency ultrasound is that the sono-lysis of HCOOH at high ultrasonic frequencies initiates Fischer-Tropsch hydrogenation of carbon monoxide. (authors)

  18. Estudio de validez predictiva

    OpenAIRE

    Barquero-Segura, José Antonio

    2003-01-01

    Proyecto de investigación Este documento es un informe ejecutivo de la información obtenida de los estudios sobre la validez predictiva de los parámetros de selección de estudiantes y su relación con el rendimiento académico. En él se presenta una síntesis de información más relevante obtenida en los últimos 17 años.

  19. Blood culture bottles are superior to lysis-centrifugation tubes for bacteriological diagnosis of spontaneous bacterial peritonitis.

    OpenAIRE

    Siersema, P D; de Marie, S; van Zeijl, J H; Bac, D J; Wilson, J H

    1992-01-01

    The conventional method of ascitic fluid culturing was compared with the bedside inoculation of ascites into blood culture bottles and into lysis-centrifugation tubes. The conventional culture method was compared with the blood culture bottle method in 31 episodes of spontaneous bacterial peritonitis (SBP). Cultures were positive with the conventional culture method in 11 (35%) episodes and with the blood culture bottle method in 26 (84%) episodes (P less than 0.001). The lysis-centrifugation...

  20. un estudio comparativo

    Directory of Open Access Journals (Sweden)

    Federico Varona

    2007-01-01

    Full Text Available La comunicación efectiva es uno de los mayores retos que tienen hoy las organizaciones y empresas tanto a nivel nacional como internacional (global. Este artículo presenta los resultados de la investigación realizada por un equipo internacional de investigadores interesados en descubrir y comparar las conductas comunicativas o estilos de comunicación de los empleados finlandeses y mexicanos cuando interactúan con sus superiores. Para ello presentamos: primero, un breve marco teórico del estudio; segundo, la metodología; tercero, los resultados del análisis estadístico comparativo entre los empleados de Finlandia y México; cuarto, las conclusiones generales y su explicación cultural; y quinto, las implicaciones teóricas y prácticas de este estudio con respecto a las competencias comunicativas necesarias para la comunicación efectiva entre empleados y superiores tanto en organizaciones nacionales como internacionales (globales.

  1. Leukocyte Lysis and Cytokine Induction by the Human Sexually Transmitted Parasite Trichomonas vaginalis.

    Directory of Open Access Journals (Sweden)

    Frances Mercer

    2016-08-01

    Full Text Available Trichomonas vaginalis (Tv is an extracellular protozoan parasite that causes the most common non-viral sexually transmitted infection: trichomoniasis. While acute symptoms in women may include vaginitis, infections are often asymptomatic, but can persist and are associated with medical complications including increased HIV susceptibility, infertility, pre-term labor, and higher incidence of cervical cancer. Heightened inflammation resulting from Tv infection could account for these complications. Effective cellular immune responses to Tv have not been characterized, and re-infection is common, suggesting a dysfunctional adaptive immune response. Using primary human leukocyte components, we have established an in vitro co-culture system to assess the interaction between Tv and the cells of the human immune system. We determined that in vitro, Tv is able to lyse T-cells and B-cells, showing a preference for B-cells. We also found that Tv lysis of lymphocytes was mediated by contact-dependent and soluble factors. Tv lysis of monocytes is far less efficient, and almost entirely contact-dependent. Interestingly, a common symbiont of Tv, Mycoplasma hominis, did not affect cytolytic activity of the parasite, but had a major impact on cytokine responses. M. hominis enabled more diverse inflammatory cytokine secretion in response to Tv and, of the cytokines tested, Tv strains cleared of M. hominis induced only IL-8 secretion from monocytes. The quality of the adaptive immune response to Tv is therefore likely influenced by Tv symbionts, commensals, and concomitant infections, and may be further complicated by direct parasite lysis of effector immune cells.

  2. Leukocyte Lysis and Cytokine Induction by the Human Sexually Transmitted Parasite Trichomonas vaginalis

    Science.gov (United States)

    Mercer, Frances; Diala, Fitz Gerald I.; Chen, Yi-Pei; Molgora, Brenda M.; Ng, Shek Hang; Johnson, Patricia J.

    2016-01-01

    Trichomonas vaginalis (Tv) is an extracellular protozoan parasite that causes the most common non-viral sexually transmitted infection: trichomoniasis. While acute symptoms in women may include vaginitis, infections are often asymptomatic, but can persist and are associated with medical complications including increased HIV susceptibility, infertility, pre-term labor, and higher incidence of cervical cancer. Heightened inflammation resulting from Tv infection could account for these complications. Effective cellular immune responses to Tv have not been characterized, and re-infection is common, suggesting a dysfunctional adaptive immune response. Using primary human leukocyte components, we have established an in vitro co-culture system to assess the interaction between Tv and the cells of the human immune system. We determined that in vitro, Tv is able to lyse T-cells and B-cells, showing a preference for B-cells. We also found that Tv lysis of lymphocytes was mediated by contact-dependent and soluble factors. Tv lysis of monocytes is far less efficient, and almost entirely contact-dependent. Interestingly, a common symbiont of Tv, Mycoplasma hominis, did not affect cytolytic activity of the parasite, but had a major impact on cytokine responses. M. hominis enabled more diverse inflammatory cytokine secretion in response to Tv and, of the cytokines tested, Tv strains cleared of M. hominis induced only IL-8 secretion from monocytes. The quality of the adaptive immune response to Tv is therefore likely influenced by Tv symbionts, commensals, and concomitant infections, and may be further complicated by direct parasite lysis of effector immune cells. PMID:27529696

  3. Tumor Lysis Syndrome (TLS following intrathecal chemotherapy in a child with acute myelogenous leukemia (AML

    Directory of Open Access Journals (Sweden)

    Chana L. Glasser, MD

    2017-01-01

    Full Text Available Tumor Lysis Syndrome (TLS is a well-known complication of induction therapy for hematologic malignancies. It is characterized by rapid breakdown of malignant white blood cells (WBCs leading to metabolic derangements and serious morbidity if left untreated. Most commonly, TLS is triggered by systemic chemotherapy, however, there have been case reports of TLS following intrathecal (IT chemotherapy, all in patients with acute lymphoblastic leukemia (ALL/lymphoma. Here, we report the first case of a patient with acute myelogenous leukemia (AML who developed TLS following a single dose of IT cytosine arabinoside (Ara-C.

  4. Lysis of Gymnodinium breve by cultures of the green alga Nannochloris eucaryotum.

    Science.gov (United States)

    Pérez, E; Sawyers, W G; Martin, D F

    2001-01-01

    Laboratory cultures of Florida's red tide organism, Gymnodinium breve, were killed by the green alga Nannochloris eucaryotum. Studies involved organism-organism interaction as well as organism-cell-free culture (N. eucaryotum) interaction. Both studies demonstrated that N. eucaryotum adversely affected Florida's red tide organism. The lysis has been attributed to compounds called APONINs (apparent oceanic naturally occurring cytolins). N. eucaryotum crude APONIN was extracted from cell-free cultures, partially purified and fractionated. The fractions were bioassayed against G. breve, and 'fingerprints' of the deleterious fractions were obtained.

  5. Investigation of an optimal cell lysis method for the study of the zinc metalloproteome of Histoplasma capsulatum.

    Science.gov (United States)

    Donnell, Anna M; Lewis, Stephanie; Abraham, Sami; Subramanian, Kavitha; Figueroa, Julio Landero; Deepe, George S; Vonderheide, Anne P

    2017-10-01

    This work sought to assess optimal extraction conditions in the study of the metalloproteome of the dimorphic fungus Histoplasma capsulatum. One of the body's responses to H. capsulatum infection is sequestration of zinc within host macrophage (MØ), as reported by Vignesh et al. (Immunity 39:697-710, 2013) and Vignesh et al. (PLOS Pathog 9:E1003815, 2013). Thus, metalloproteins containing zinc were of greatest interest as it plays a critical role in survival of the fungus. One challenge in metalloproteomics is the preservation of the native structure of proteins to retain non-covalently bound metals. Many of the conventional cell lysis, separation, and identification techniques in proteomics are carried out under conditions that could lead to protein denaturation. Various cell lysis techniques were investigated in an effort to both maintain the metalloproteins during lysis and subsequent analysis while, at the same time, serving to be strong enough to break the cell wall, allowing access to cytosolic metalloproteins. The addition of 1% Triton x-100, a non-ionic detergent, to the lysis buffer was also studied. Seven lysis methods were considered and these included: Glass Homogenizer (H), Bead Beater (BB), Sonication Probe (SP), Vortex with 1% Triton x-100 (V, T), Vortex with no Triton x-100 (V, NT), Sonication Bath, Vortex, and 1% Triton x-100 (SB, V, T) and Sonication Bath, Vortex, and no Triton x-100 (SB, V, NT). A Qubit® Assay was used to compare total protein concentration and inductively coupled plasma-mass spectrometry (ICP-MS) was utilized for total metal analysis of cell lysates. Size exclusion chromatography coupled to ICP-MS (SEC-HPLC-ICP-MS) was used for separation of the metalloproteins in the cell lysate and the concentration of Zn over a wide molecular weight range was examined. Additional factors such as potential contamination sources were also considered. A cell lysis method involving vortexing H. capsulatum yeast cells with 500 μm glass beads

  6. Dos estudios de casos

    Directory of Open Access Journals (Sweden)

    Hernán Guillermo Salazar Morales

    1989-04-01

    Full Text Available RESUMEN A continuación se presenta dos casos de estudio en donde se puede apreciar a la forma  como se establecen  las relaciones en una organización y lo que una DECISION  puede llegar a incidir en el desarrollo de las operaciones de una empresa. Decisiones basadas en la intuición, o en corazonadas, frecuentemente conducen en dirección equivocada, ocasionando pérdidas en tiempo, personal y dinero. El proceso de toma de decisiones deben ser TAREAS ESTRUCTURADAS que se sustenten con hechos. Su ejecución debe hacerse secuencialmente y cumplir con cada uno de sus pasos. El diseño de los  casos está orientado para que  sirvan como material de aplicación en la asignatura de  taller de planeación.

  7. DE AGUA. ESTUDIO PRELIMINAR

    Directory of Open Access Journals (Sweden)

    Esther E. Pellizzari

    2015-01-01

    Full Text Available El objetivo del presente estudio fue investigar la resistencia al arsénico en cultivos puros de Pseudomonas aeruginosa , aislada de aguas subterráneas de Presidencia Roque Sáenz Peña, provincia de Chaco y evaluar la posibilidad de su uso para la remoción de este contaminante presente en las aguas subterráneas. Las cepas fueron inmovilizadas en piedra natural y se cu ltivaron en caldo de sales y 1 mgAs/L . Se observó la resistencia al arsénico y la formación de biofilm , logrando la interacción entre la s células, roca y arsénico . L a remoción de arsénico se evaluó durante 3 meses y el porcentaje de eliminación de arsénico al final del experimento fue 60%.

  8. Software for the nuclear reactor dynamics study using time series processing; Software para el estudio de la dinamica de reactores nucleares mediante el procesamiento de series temporales

    Energy Technology Data Exchange (ETDEWEB)

    Valero, Esbel T.; Montesino, Maria E. [Instituto Superior de Ciencia y Tecnologia Nuclear (ISCTN), La Habana (Cuba)

    1997-12-01

    The parametric monitoring in Nuclear Power Plant (NPP) permits the operational surveillance of nuclear reactor. The methods employed in order to process this information such as FFT, autoregressive models and other, have some limitations when those regimens in which appear strongly non-linear behaviors are analyzed. In last years the chaos theory has offered new ways in order to explain complex dynamic behaviors. This paper describes a software (ECASET) that allow, by time series processing from NPP`s acquisition system, to characterize the nuclear reactor dynamic as a complex dynamical system. Here we show using ECASET`s results the possibility of classifying the different regimens appearing in nuclear reactors. The results of several temporal series processing from real systems are introduced. This type of analysis complements the results obtained with traditional methods and can constitute a new tool for monitoring nuclear reactors. (author). 13 refs., 3 figs.

  9. Treatment of cutting fluid: comparative study of different processes of recycling; Tratamiento de fluidos de corte estudio comparativo de los diferentes procesos de reciclaje

    Energy Technology Data Exchange (ETDEWEB)

    Labarta Carreno, C.E.; Ipinar, E.

    1997-12-31

    The environmental concerning about cutting fluid (commonly known in Spain as taladrines) is deeply analyzed in this paper by the authors. They describe the history of these hazardous effluents what kind there, are their characteristics, and finally they study comparatively the different industrial processes for their treatment. (Author) 7 refs.

  10. Resistance of some leukemic blasts to lysis by lymphokine activated killer (LAK) cells

    Energy Technology Data Exchange (ETDEWEB)

    Panayotides, P; Sjoegren, A -M; Reizenstein, P; Porwit, A. Immunopathology Lab., Dept. of Pathology, Karolinska Hospital, Stockholm; Wasserman, J

    1988-01-01

    Peripheral blood mononuclear cells (PBMC) from healthy donors and AML patients in remission were stimulated with phytohemagglutinin (PHA) and recombinant interleukin-2 (IL-2). These stimulated cells (lymphokine activated killer (LAK) cells) showed increased DNA synthesis as measured by /sup 3/H-Thymidine uptake. A synergistic effect of PHA and IL-2 was found. LAK cells' ability to kill acute myeloid leukemia (AML) blasts was investigated by the /sup 51/Cr release assay. LAK cells showed a cytotoxicity (over 10% specific /sup 51/Cr release) against 9/12 leukemic blasts, even at effector/target (E/T) ratios as low as 5:1. However, on average only 22.2% (SD 11.8) and 36.5% (SD 12.5) /sup 51/Cr release were obtained in 4- and 18-hour cytotoxicity assays, respectively, at an E/T ratio of 20:1. Leukemic blasts in 3/12 AML cases and normal PBMC were entirely resistant to lysis, even at an E/T ratio of 80:1. Susceptibility to lysis was not correlated to peanut-agglutinin receptor expression. LAK cells were more cytotoxic towards the K-562 cell line (natural killer activity) than unstimulated PBMC.

  11. Capacity of tumor necrosis factor to augment lymphocyte-mediated tumor cell lysis of malignant mesothelioma

    International Nuclear Information System (INIS)

    Bowman, R.V.; Manning, L.S.; Davis, M.R.; Robinson, B.W.

    1991-01-01

    Recombinant human tumor necrosis factor (rHuTNF) was evaluated both for direct anti-tumor action against human malignant mesothelioma and for its capacity to augment the generation and lytic phases of lymphocyte-mediated cytotoxicity against this tumor. rHuTNF was directly toxic by MTT assay to one of two mesothelioma cell lines evaluated, but had no effect on susceptibility to subsequent lymphocyte-mediated lysis of either line. TNF alone was incapable of generating anti-mesothelioma lymphokine-activated killer cell (LAK) activity. Furthermore, it did not augment the degree or LAK activity produced by submaximal interleukin-2 (IL-2) concentrations nor did it augment lysis of mesothelioma cells by natural killer (NK) or LAK effector cells during the 4-hr 51chromium release cytolytic reaction. The studies also suggest that mesothelioma targets are less responsive to TNF plus submaximal IL-2 concentrations than the standard LAK sensitive target Daudi, raising the possibility that intermediate LAK sensitive tumors such as mesothelioma may require separate and specific evaluation in immunomodulation studies. This in vitro study indicates that use of low-dose rHuTNF and IL-2 is unlikely to be an effective substitute for high-dose IL-2 in generation and maintenance of LAK activity in adoptive immunotherapy for mesothelioma

  12. Biological activity analysis of native and recombinant streptokinase using clot lysis and chromogenic substrate assay.

    Science.gov (United States)

    Mahboubi, Arash; Sadjady, Seyyed Kazem; Mirzaei Saleh Abadi, Mohammad; Azadi, Saeed; Solaimanian, Roya

    2012-01-01

    DETERMINATION OF STREPTOKINASE ACTIVITY IS USUALLY ACCOMPLISHED THROUGH TWO ASSAY METHODS: a) Clot lysis, b) Chromogenic substrate assay. In this study the biological activity of two streptokinase products, namely Streptase®, which is a native product and Heberkinasa®, which is a recombinant product, was determined against the third international reference standard using the two forementioned assay methods. The results indicated that whilst the activity of Streptase® was found to be 101 ± 4% and 97 ± 5% of the label claim with Clot lysis and Chromogenic substrate assay respectively, for Heberkinasa® the potency values obtained were 42 ± 5% and 92.5 ± 2% of the label claim respectively. To shed some light on the reason for this finding, the n-terminal sequence of the streptokinase molecules present in the two products was determined. The results showed slight differences in the amino acid sequence of the recombinant product in comparison to the native one at the amino terminus. This finding supports those of other workers who found that n-terminal sequence of the streptokinase molecule can have significant effect on the activity of this protein.

  13. HLA-E-expressing pluripotent stem cells escape allogeneic responses and lysis by NK cells.

    Science.gov (United States)

    Gornalusse, Germán G; Hirata, Roli K; Funk, Sarah E; Riolobos, Laura; Lopes, Vanda S; Manske, Gabriel; Prunkard, Donna; Colunga, Aric G; Hanafi, Laïla-Aïcha; Clegg, Dennis O; Turtle, Cameron; Russell, David W

    2017-08-01

    Polymorphisms in the human leukocyte antigen (HLA) class I genes can cause the rejection of pluripotent stem cell (PSC)-derived products in allogeneic recipients. Disruption of the Beta-2 Microglobulin (B2M) gene eliminates surface expression of all class I molecules, but leaves the cells vulnerable to lysis by natural killer (NK) cells. Here we show that this 'missing-self' response can be prevented by forced expression of minimally polymorphic HLA-E molecules. We use adeno-associated virus (AAV)-mediated gene editing to knock in HLA-E genes at the B2M locus in human PSCs in a manner that confers inducible, regulated, surface expression of HLA-E single-chain dimers (fused to B2M) or trimers (fused to B2M and a peptide antigen), without surface expression of HLA-A, B or C. These HLA-engineered PSCs and their differentiated derivatives are not recognized as allogeneic by CD8 + T cells, do not bind anti-HLA antibodies and are resistant to NK-mediated lysis. Our approach provides a potential source of universal donor cells for applications where the differentiated derivatives lack HLA class II expression.

  14. HLA-E-expressing pluripotent stem cells escape allogeneic responses and lysis by NK cells

    Science.gov (United States)

    Gornalusse, Germán G.; Hirata, Roli K.; Funk, Sarah; Riolobos, Laura; Lopes, Vanda S.; Manske, Gabriel; Prunkard, Donna; Colunga, Aric G.; Hanafi, Laïla-Aïcha; Clegg, Dennis O.; Turtle, Cameron; Russell, David W.

    2017-01-01

    Polymorphisms in the human leukocyte antigen (HLA) class I genes can cause the rejection of pluripotent stem cell (PSC)-derived products in allogeneic recipients. Disruption of the Beta-2 Microglobulin (B2M) gene eliminates surface expression of all class I molecules, but leaves the cells vulnerable to lysis by natural killer (NK) cells. Here we show that this ‘missing self’ response can be prevented by forced expression of minimally polymorphic HLA-E molecules. We use adeno-associated virus (AAV)-mediated gene editing to knock in HLA-E genes at the B2M locus in human PSCs in a manner that confers inducible, regulated, surface expression of HLA-E single-chain dimers (fused to B2M) or trimers (fused to B2M and a peptide antigen), without surface expression of HLA-A, B or C. These HLA-engineered PSCs and their differentiated derivatives are not recognized as allogeneic by CD8+ T cells, do not bind anti-HLA antibodies, and are resistant to NK-mediated lysis. Our approach provides a potential source of universal donor cells for applications where the differentiated derivatives lack HLA class II expression. PMID:28504668

  15. Clotrimazole enhances lysis of human erythrocytes induced by t-BHP.

    Science.gov (United States)

    Lisovskaya, Irene L; Shcherbachenko, Irina M; Volkova, Rimma I; Ataullakhanov, Fazoil I

    2009-08-14

    Clotrimazole (CLT) is an antifungal and antimalarial agent also effective as a Gardos channel inhibitor. In addition, CLT possesses antitumor properties. Recent data provide evidence that CLT forms a complex with heme (hemin), which produces a more potent lytic effect than heme alone. This study addressed the effect of CLT on the lysis of normal human erythrocytes induced by tert-butyl hydroperoxide (t-BHP). For the first time, it was shown that 10 microM CLT significantly enhanced the lytic effect of t-BHP on erythrocytes in both Ca(2+)-containing and Ca(2+)-free media, suggesting that the effect is not related to Gardos channels. CLT did not affect the rate of free radical generation, the kinetics of GSH degradation, methemoglobin formation and TBARS generation; therefore, we concluded that CLT does not cause additional oxidative damage to erythrocytes treated with t-BHP. It is tempted to speculate that CLT enhances t-BHP-induced changes in erythrocyte volume and lysis largely by forming a complex with hemin released during hemoglobin oxidation in erythrocytes: the CLT-hemin complex destabilizes the cell membrane more potently than hemin alone. If so, the effect of CLT on cell membrane damage during free-radical oxidation may be used to increase the efficacy of antitumor therapy.

  16. Revisiting bistability in the lysis/lysogeny circuit of bacteriophage lambda.

    Directory of Open Access Journals (Sweden)

    Michael Bednarz

    Full Text Available The lysis/lysogeny switch of bacteriophage lambda serves as a paradigm for binary cell fate decision, long-term maintenance of cellular state and stimulus-triggered switching between states. In the literature, the system is often referred to as "bistable." However, it remains unclear whether this term provides an accurate description or is instead a misnomer. Here we address this question directly. We first quantify transcriptional regulation governing lysogenic maintenance using a single-cell fluorescence reporter. We then use the single-cell data to derive a stochastic theoretical model for the underlying regulatory network. We use the model to predict the steady states of the system and then validate these predictions experimentally. Specifically, a regime of bistability, and the resulting hysteretic behavior, are observed. Beyond the steady states, the theoretical model successfully predicts the kinetics of switching from lysogeny to lysis. Our results show how the physics-inspired concept of bistability can be reliably used to describe cellular phenotype, and how an experimentally-calibrated theoretical model can have accurate predictive power for cell-state switching.

  17. Isolation and Expression of the Lysis Genes of Actinomyces naeslundii Phage Av-1

    Science.gov (United States)

    Delisle, Allan L.; Barcak, Gerard J.; Guo, Ming

    2006-01-01

    Like most gram-positive oral bacteria, Actinomyces naeslundii is resistant to salivary lysozyme and to most other lytic enzymes. We are interested in studying the lysins of phages of this important oral bacterium as potential diagnostic and therapeutic agents. To identify the Actinomyces phage genes encoding these species-specific enzymes in Escherichia coli, we constructed a new cloning vector, pAD330, that can be used to enrich for and isolate phage holin genes, which are located adjacent to the lysin genes in most phage genomes. Cloned holin insert sequences were used to design sequencing primers to identify nearby lysin genes by using whole phage DNA as the template. From partial digestions of A. naeslundii phage Av-1 genomic DNA we were able to clone, in independent experiments, inserts that complemented the defective λ holin in pAD330, as evidenced by extensive lysis after thermal induction. The DNA sequence of the inserts in these plasmids revealed that both contained the complete lysis region of Av-1, which is comprised of two holin-like genes, designated holA and holB, and an endolysin gene, designated lysA. We were able to subclone and express these genes and determine some of the functional properties of their gene products. PMID:16461656

  18. All-in-one nanowire-decorated multifunctional membrane for rapid cell lysis and direct DNA isolation.

    KAUST Repository

    So, Hongyun

    2014-11-24

    This paper describes a handheld device that uses an all-in-one membrane for continuous mechanical cell lysis and rapid DNA isolation without the assistance of power sources, lysis reagents, and routine centrifugation. This nanowire-decorated multifunctional membrane was fabricated to isolate DNA by selective adsorption to silica surface immediately after disruption of nucleus membranes by ultrasharp tips of nanowires for a rapid cell lysis, and it can be directly assembled with commercial syringe filter holders. The membrane was fabricated by photoelectrochemical etching to create microchannel arrays followed by hydrothermal synthesis of nanowires and deposition of silica. The proposed membrane successfully purifies high-quality DNA within 5 min, whereas a commercial purification kit needs more than an hour.

  19. All-in-one nanowire-decorated multifunctional membrane for rapid cell lysis and direct DNA isolation.

    KAUST Repository

    So, Hongyun; Lee, Kunwoo; Murthy, Niren; Pisano, Albert P

    2014-01-01

    This paper describes a handheld device that uses an all-in-one membrane for continuous mechanical cell lysis and rapid DNA isolation without the assistance of power sources, lysis reagents, and routine centrifugation. This nanowire-decorated multifunctional membrane was fabricated to isolate DNA by selective adsorption to silica surface immediately after disruption of nucleus membranes by ultrasharp tips of nanowires for a rapid cell lysis, and it can be directly assembled with commercial syringe filter holders. The membrane was fabricated by photoelectrochemical etching to create microchannel arrays followed by hydrothermal synthesis of nanowires and deposition of silica. The proposed membrane successfully purifies high-quality DNA within 5 min, whereas a commercial purification kit needs more than an hour.

  20. Procesos indentitarios en el paisaje contemporáneo. Caso de estudio de la Vega de Granada. / Identity processes in the contemporary landscape. Case of study of the Vega de Granada.

    Directory of Open Access Journals (Sweden)

    Maria Teresa Zapiain Aizpuru

    2011-09-01

    Full Text Available ResumenConsiderando la distinción de significado establecida entre los conceptos de espacio, territorio y paisaje, y su relación indisociable con la cultura y la identidad, en su primera parte, este artículo expone una propuesta teórica de interpretación de los procesos identitarios vinculados con el territorio. En la segunda parte, se explora, por medio de un estudio empírico en la Vega de Granada, la relación entre territorio e identidad, así como la influencia de ciertas transformaciones socioeconómicas y culturales en los procesos de identificación con el lugar.Palabras clave Territorio, paisaje, cultura, identidad, Vega de Granada. AbstractConsidering the meaningful distinction drawn between the concepts of space, territory and landscape, and its inseparable relationship with culture and identity, in its first part, this paper presents a theoretical proposal for the interpretation of identity processes associated with the territory. In the second part, is explored the relation between territory and identity, as well as the influence of certain socio-economic and cultural transformations in identity processes through an empirical study of the Vega de GranadaKeywordsTerritory, landscape, culture, identity, Vega de Granada.

  1. Desalination of brackish waters by electrodialysis. I. Process variable studies; Desalacion de aguas salobres por electrodialisis. I. Estudio de las variables del proceso

    Energy Technology Data Exchange (ETDEWEB)

    Alonso Lopez, J; Sainz Sastre, J A

    1972-07-01

    This study was carried out with solutions of 5000, 3000 and 1000 ppm of NaCl, and 3000 and 1000 ppm of NaSO{sub 4}. A stack-pack of 20 pairs membranes, Nepton lonics 61 AZL 183 -cationic- and 111 BZL 183 -anionic- with 220 cm{sup 2}/membrane was used. For the above mentioned Solutions the following values were determined: Limiting current density; the values of n and K in the expression which relates the L.C.D. with flow rate; dependence of spent energy on flow rate; spent energy and time of operation versus initial current and concentration of the solution, and finally the influence of the concentration potential on the electrodialytic process. A discussion of the results obtained is included. (Author) 18 refs.

  2. Study of the ortho-positron annihilation process in zeolite Y; Estudio del proceso de aniquilacion de ortopositronio en zeolita Y

    Energy Technology Data Exchange (ETDEWEB)

    Alvarado D, M E

    2010-07-01

    For several years a great interest has existed for the study of the natural and synthetic zeolites due to its properties. The porosity, one of their main characteristics allows that these materials are used as molecular sieves, catalysts, gases drying, etc. In order to investigating the porosity and other zeolite properties one carries out the study of the process of positron annihilation lifetime spectroscopy (Pals). This is a technique that provides information about the size and the pores form since is highly sensitive to the free volume and the superficial area of those porous materials as the zeolites. The study began with the elaboration of zeolite Y tablets in a hydraulic press where different pressures (from 0 to 1.26 GPa) and masses (70, 80 and 100 mg) were proven to obtain the estimate porosity of each tablet. A graph was elaborated and the effect of the mass and pressure with regard to the zeolite porosity was analyzed. Later on, the powder and tablets of 70 mg were characterized by means of X-ray diffraction (the glass size, interplanar distance, length and the volume of the unitary cell); scanning electron microscopy (the particles size and morphology); thermo gravimetric analysis (dehydration temperature and the stability up to 700 C) and the Brunauer Emmett Teller method (specific area). After the zeolite Y tablets characterization was carried out the positron annihilation process by means of Pals where its free volume of zeolite Y was analyzed, which includes to the structural cavities and the interparticle volume. The powdered zeolite was analyzed to different experimental conditions (preparation of the sample and the Pals equipment) to obtain the optimal conditions (a window with a time of 400 ns and a enlarged energy window) of analysis. On the other hand, the tablets were analyzed under optimal conditions to obtain the four components of time and intensity ({tau}, {Iota}), result of the different ways of positrons annihilation in the zeolite

  3. Measurement of electrochemical noise for the study of corrosion processes of metallic alloys; Medida de ruido electroquimico para el estudio de rocesoso de corrosion de aleaciones metalicas

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez-Amaya, J. M.; Bethencourt, M.; Gonzalez-Rovira, L.; Botana, F. J.

    2009-07-01

    Electrochemical noise (EN) is a technique that allows the estimation of both the rate and the corrosion mechanism of different metallic alloys by means of the measurement and the analysis of the fluctuations of current and voltage. Its main advantage against other electrochemical techniques is that during the measurement process, the corrosive systems under study are not instrumentally disturbed, and therefore, the systems are kept at their natural corrosion potential. Two steps are necessary to use this technique: measurement and analysis of the EN signals. In this paper, the most important concepts related only to the measurement of EN are revised. The parameters most employed in the literature to analyse the EN signals will be described in another paper. In the present article, the experimental devices normally used to measure EN signals are firstly analysed. Subsequently, the most important properties of the EN signals are studied. Finally, the external sources of instrumental noise that can affect to the EN signals are described. (Author) 65 refs.

  4. Analysis of five streptokinase formulations using the euglobulin lysis test and the plasminogen activation assay

    Directory of Open Access Journals (Sweden)

    Couto L.T.

    2004-01-01

    Full Text Available Streptokinase, a 47-kDa protein isolated and secreted by most group A, C and G ß-hemolytic streptococci, interacts with and activates human protein plasminogen to form an active complex capable of converting other plasminogen molecules to plasmin. Our objective was to compare five streptokinase formulations commercially available in Brazil in terms of their activity in the in vitro tests of euglobulin clot formation and of the hydrolysis of the plasmin-specific substrate S-2251(TM. Euglobulin lysis time was determined using a 96-well microtiter plate. Initially, human thrombin (10 IU/ml and streptokinase were placed in individual wells, clot formation was initiated by the addition of plasma euglobulin, and turbidity was measured at 340 nm every 30 s. In the second assay, plasminogen activation was measured using the plasmin-specific substrate S-2251(TM. Streptase(TM was used as the reference formulation because it presented the strongest fibrinolytic activity in the euglobulin lysis test. The Unitinase(TM and Solustrep(TM formulations were the weakest, showing about 50% activity compared to the reference formulation. All streptokinases tested activated plasminogen but significant differences were observed. In terms of total S-2251(TM activity per vial, Streptase(TM (75.7 ± 5.0 units and Streptonase(TM (94.7 ± 4.6 units had the highest activity, while Unitinase(TM (31.0 ± 2.4 units and Strek(TM (32.9 ± 3.3 units had the weakest activity. Solustrep(TM (53.3 ± 2.7 units presented intermediate activity. The variations among the different formulations for both euglobulin lysis test and chromogenic substrate hydrolysis correlated with the SDS-PAGE densitometric results for the amount of 47-kDa protein. These data show that the commercially available clinical streptokinase formulations vary significantly in their in vitro activity. Whether these differences have clinical implications needs to be investigated.

  5. Estudio comparativo de los procesos de socialización alimentaria de adolescentes diagnosticados y no diagnosticados de trastornos alimentarios (Comparative study of food socialization processes of teenagers diagnosed / not diagnosed with eating disorders

    Directory of Open Access Journals (Sweden)

    Eva Zafra Aparici

    2015-05-01

    Full Text Available Resumen: En este artículo se exponen los resultados de un estudio cualitativo y cuantitativo en el que se comparan los procesos de socialización alimentaria de adolescentes diagnosticados de algún Trastornos del Comportamiento Alimentario (TCA con otros no diagnosticados. Se analizan los elementos en común y divergencia entre sus respectivas experiencias de aprendizaje alimentario, lo cual nos ha permitido conocer y profundizar en las causas socioculturales de los TCA; causas que, indiscutiblemente, van ligadas a los sentidos y significados que adquiere la alimentación en nuestro contexto actual y cotidiano.Abstract: In this paper are exposed the results of a qualitative and quantitative study that compare the food socialization processes of boys and girls diagnosed of some eating disorder with others not diagnosed. We have analyzed the common elements and divergence between food learning experiences. This analysis has allowed us to know and deepen in the sociocultural causes of these problems, indisputably linked to the senses and meanings that food acquires in our current context.

  6. Procesos de búsqueda de información y zonas de intervención: un estudio de investigadores en Literatura Information seeking process: A study of Literature researchers

    Directory of Open Access Journals (Sweden)

    Susana Romanos de Tiratel

    2002-06-01

    Full Text Available Este artículo informa sobre un estudio exploratorio de un grupo de personas, graduadas en Literatura, que, durante un lapso prolongado de sus vidas, se dedican a investigar un tema para obtener el doctorado. Se identifican las estrategias involucradas en el proceso de búsqueda de información (PBI en actividades complejas; se establece que las actividades del PBI se combinan y se influyen dinámica y recurrentemente; se identifican las zonas de intervención en el PBI; se utilizan modelos combinados para describir los momentos del PBI y verificar la viabilidad de su aplicación.This paper informs about an exploratory study referred to a group of literature postgraduates who, for many years, were devoted to research a topic in order to obtain a doctor degree. The involved strategies in the information seeking process (ISP in complex activities are identified; it is established that the ISP activities combined themselves and are dynamically and recurrently influenced by each other; the ISP zones of intervention are identified; combined models are used to describe the ISP moments, and to verify the models application viability.

  7. DIAGNÓSTICO DEL PROCESO DE COMUNICACIÓN ORGANIZACIONAL. CASO DE ESTUDIO QUIMEFA / DIAGNOSIS OF THE ORGANIZATIONAL COMMUNICATION PROCESS. STUDY CASE OF QUIMEFA

    Directory of Open Access Journals (Sweden)

    Mónica Queris-Rojas

    2012-04-01

    Full Text Available La comunicación es un proceso que ocurre en cualquier tipo de organización y además, es el que involucra permanentemente, tanto a los empleados como a los usuarios. El trabajo realizado propone una metodología conformada por 4 pasos, la cual se basa en el Decreto 281, las Normas Cubanas 3000:2007, la norma ISO 9001:2008, la mejora continua y la función de directivos como principales gestores de la comunicación dentro de una entidad. Dicha metodología permitió definir las primeras bases sobre las cuales sustentar el diseño del sistema de comunicación empresarial en la Oficina Central del Grupo Empresarial Farmacéutico QUIMEFA. Con su aplicación se constatan los elementos que aún hacen deficiente la comunicación y se llega a la creación de un plan para corregir la situación comunicativa actual, aunque se propone la necesidad de la búsqueda de un método para aplicar las medidas propuestas de manera eficaz.AbstractThe communication is a process that happens in any type of organization and it also involves permanently, both the employees and the users. The carried out work propose a methodology constituted by 4 steps, which is based on the Decree 281, the Cuban Standards 3000:2007, the ISO 9001:2008 Standard, the continuous improvement and the function of managers as main agents of the communication inside an organization. This methodology allowed the definition of the first bases for the design of the managerial communication system in the Head Office of the Pharmaceutical Managerial Group QUIMEFA. Its implementation permits to verify the elements that still make the communication deficient and a plan is created in order to correct the current communicative situation, although it is suggested the need of searching a method that allows the application of the proposed measures effectively.

  8. MEXCODE* CASO DE ESTUDIO

    Directory of Open Access Journals (Sweden)

    Gabriel Arturo García Gómez

    2000-01-01

    Full Text Available El caso plantea la reactivación de un proyecto que se presentó en el año 1995 como alternativa para el desarrollo del centro del Valle y mejora de la eficiencia en el manejo del comercio exterior de la Cuenca Pacífica. El proyecto se revivió a raíz de la necesidad de la compañía patrocinadora de mejorar sus flujos de caja proyectados y la reciente autorización para su funcionamiento. El proyecto es el popularmente denominado “Puerto Seco” de la ciudad de Guadalajara de Buga, internamente nombrado Mexcode. Los estudios iniciales estaban encaminados a que el proyecto fuera manejado por el municipio de Buga, sin embargo, posteriormente se vio la necesidad de buscar que pasara a manos de la empresa privada. El municipio, después de analizar las propuestas de varios inversionistas de la región en las cuales se evaluaba la ubicación dentro de la zona de los predios de sus empresas, su área, proximidad a la línea férrea y a la doble calzada Buga-Tuluá (sitio de paso del 80% de la carga de importación y exportación por Buenaventura y evaluar aspectos relativos a su trayectoria empresarial y compromiso con el desarrollo de la región, decidió otorgarle el proyecto al Grupo Empresarial Agri-Supplies S.A.

  9. Estudio del CH interestelar

    Science.gov (United States)

    Olano, C.; Lemarchand, G.; Sanz, A. J.; Bava, J. A.

    El objetivo principal de este proyecto consiste en el estudio de la distribución y abundancia del CH en nubes interestelares a través de la observación de las líneas hiperfinas del CH en 3,3 GHz. El CH es una molécula de amplia distribución en el espacio interestelar y una de las pocas especies que han sido observadas tanto con técnicas de radio como ópticas. Desde el punto de vista tecnológico se ha desarrollado un cabezal de receptor que permitirá la realización de observaciones polarimétricas en la frecuencia de 3,3 GHz, con una temperatura del sistema de 60 K y un ancho de banda de 140 MHz, y que será instalado en el foco primario de la antena parabólica del IAR. El cabezal del receptor es capaz de detectar señales polarizadas, separando las componentes de polarización circular derecha e izquierda. Para tal fin el cabezal consta de dos ramas receptoras que amplificarán la señal y la trasladarán a una frecuencia más baja (frecuencia intermedia), permitiendo de esa forma un mejor transporte de la señal a la sala de control para su posterior procesamiento. El receptor además de tener características polarimétricas, podrá ser usado en el continuo y en la línea, utilizando las ventajas observacionales y de procesamiento de señal que actualmente posee el IAR.

  10. A single lysis solution for the analysis of tissue samples by different proteomic technologies

    DEFF Research Database (Denmark)

    Gromov, P.; Celis, J.E.; Gromova, I.

    2008-01-01

    -based proteomics (reverse-phase lysate arrays or direct antibody arrays), allowing the direct comparison of qualitative and quantitative data yielded by these technologies when applied to the same samples. The usefulness of the CLB1 solution for gel-based proteomics was further established by 2D PAGE analysis...... dissease, is driving scientists to increasingly use clinically relevant samples for biomarker and target discovery. Tissues are heterogeneous and as a result optimization of sample preparation is critical for generating accurate, representative, and highly reproducible quantitative data. Although a large...... number of protocols for preparation of tissue lysates has been published, so far no single recipe is able to provide a "one-size fits all" solubilization procedure that can be used to analyse the same lysate using different proteomics technologies. Here we present evidence showing that cell lysis buffer...

  11. Hyphal growth of phagocytosed Fusarium oxysporum causes cell lysis and death of murine macrophages.

    Directory of Open Access Journals (Sweden)

    Katja Schäfer

    Full Text Available Fusarium oxysporum is an important plant pathogen and an opportunistic pathogen of humans. Here we investigated phagocytosis of F. oxysporum by J774.1 murine cell line macrophages using live cell video microscopy. Macrophages avidly migrated towards F. oxysporum germlings and were rapidly engulfed after cell-cell contact was established. F. oxysporum germlings continued hyphal growth after engulfment by macrophages, leading to associated macrophage lysis and escape. Macrophage killing depended on the multiplicity of infection. After engulfment, F. oxysporum inhibited macrophages from completing mitosis, resulting in large daughter cells fused together by means of a F. oxysporum hypha. These results shed new light on the initial stages of Fusarium infection and the innate immune response of the mammalian host.

  12. Hyphal growth of phagocytosed Fusarium oxysporum causes cell lysis and death of murine macrophages.

    Science.gov (United States)

    Schäfer, Katja; Bain, Judith M; Di Pietro, Antonio; Gow, Neil A R; Erwig, Lars P

    2014-01-01

    Fusarium oxysporum is an important plant pathogen and an opportunistic pathogen of humans. Here we investigated phagocytosis of F. oxysporum by J774.1 murine cell line macrophages using live cell video microscopy. Macrophages avidly migrated towards F. oxysporum germlings and were rapidly engulfed after cell-cell contact was established. F. oxysporum germlings continued hyphal growth after engulfment by macrophages, leading to associated macrophage lysis and escape. Macrophage killing depended on the multiplicity of infection. After engulfment, F. oxysporum inhibited macrophages from completing mitosis, resulting in large daughter cells fused together by means of a F. oxysporum hypha. These results shed new light on the initial stages of Fusarium infection and the innate immune response of the mammalian host.

  13. Continuous nucleus extraction by optically-induced cell lysis on a batch-type microfluidic platform.

    Science.gov (United States)

    Huang, Shih-Hsuan; Hung, Lien-Yu; Lee, Gwo-Bin

    2016-04-21

    The extraction of a cell's nucleus is an essential technique required for a number of procedures, such as disease diagnosis, genetic replication, and animal cloning. However, existing nucleus extraction techniques are relatively inefficient and labor-intensive. Therefore, this study presents an innovative, microfluidics-based approach featuring optically-induced cell lysis (OICL) for nucleus extraction and collection in an automatic format. In comparison to previous micro-devices designed for nucleus extraction, the new OICL device designed herein is superior in terms of flexibility, selectivity, and efficiency. To facilitate this OICL module for continuous nucleus extraction, we further integrated an optically-induced dielectrophoresis (ODEP) module with the OICL device within the microfluidic chip. This on-chip integration circumvents the need for highly trained personnel and expensive, cumbersome equipment. Specifically, this microfluidic system automates four steps by 1) automatically focusing and transporting cells, 2) releasing the nuclei on the OICL module, 3) isolating the nuclei on the ODEP module, and 4) collecting the nuclei in the outlet chamber. The efficiency of cell membrane lysis and the ODEP nucleus separation was measured to be 78.04 ± 5.70% and 80.90 ± 5.98%, respectively, leading to an overall nucleus extraction efficiency of 58.21 ± 2.21%. These results demonstrate that this microfluidics-based system can successfully perform nucleus extraction, and the integrated platform is therefore promising in cell fusion technology with the goal of achieving genetic replication, or even animal cloning, in the near future.

  14. Stabilizing additives added during cell lysis aid in the solubilization of recombinant proteins.

    Directory of Open Access Journals (Sweden)

    David J Leibly

    Full Text Available Insoluble recombinant proteins are a major issue for both structural genomics and enzymology research. Greater than 30% of recombinant proteins expressed in Escherichia coli (E. coli appear to be insoluble. The prevailing view is that insolubly expressed proteins cannot be easily solubilized, and are usually sequestered into inclusion bodies. However, we hypothesize that small molecules added during the cell lysis stage can yield soluble protein from insoluble protein previously screened without additives or ligands. We present a novel screening method that utilized 144 additive conditions to increase the solubility of recombinant proteins expressed in E. coli. These selected additives are natural ligands, detergents, salts, buffers, and chemicals that have been shown to increase the stability of proteins in vivo. We present the methods used for this additive solubility screen and detailed results for 41 potential drug target recombinant proteins from infectious organisms. Increased solubility was observed for 80% of the recombinant proteins during the primary and secondary screening of lysis with the additives; that is 33 of 41 target proteins had increased solubility compared with no additive controls. Eleven additives (trehalose, glycine betaine, mannitol, L-Arginine, potassium citrate, CuCl(2, proline, xylitol, NDSB 201, CTAB and K(2PO(4 solubilized more than one of the 41 proteins; these additives can be easily screened to increase protein solubility. Large-scale purifications were attempted for 15 of the proteins using the additives identified and eight (40% were prepared for crystallization trials during the first purification attempt. Thus, this protocol allowed us to recover about a third of seemingly insoluble proteins for crystallography and structure determination. If recombinant proteins are required in smaller quantities or less purity, the final success rate may be even higher.

  15. Prediction of recurrent venous thromboembolism by clot lysis time: a prospective cohort study.

    Science.gov (United States)

    Traby, Ludwig; Kollars, Marietta; Eischer, Lisbeth; Eichinger, Sabine; Kyrle, Paul A

    2012-01-01

    Venous thromboembolism (VTE) is a chronic disease, which tends to recur. Whether an abnormal fibrinolytic system is associated with an increased risk of VTE is unclear. We assessed the relationship between fibrinolytic capacity (reflected by clot lysis time [CLT]) and risk of recurrent VTE. We followed 704 patients (378 women; mean age 48 yrs) with a first unprovoked VTE for an average of 46 months after anticoagulation withdrawal. Patients with natural coagulation inhibitor deficiency, lupus anticoagulant, cancer, homozygosity for factor V Leiden or prothrombin mutation, or requirement for indefinite anticoagulation were excluded. Study endpoint was symptomatic recurrent VTE. For measurement of CLT, a tissue factor-induced clot was lysed by adding tissue-type plasminogen activator. Time between clot formation and lysis was determined by measuring the turbidity. 135 (19%) patients had recurrent VTE. For each increase in CLT of 10 minutes, the crude relative risk (RR) of recurrence was 1.13 (95% CI 1.02-1.25; p = 0.02) and was 1.08 (95% CI 0.98-1.20; p = 0.13) after adjustment for age and sex. For women only, the adjusted RR was 1.14 (95% CI, 0.91-1.42, p = 0.22) for each increase in CLT of 10 minutes. CLT values in the 4(th) quartile of the female patient population, as compared to values in the 1(st) quartile, conferred a risk of recurrence of 3.28 (95% CI, 1.07-10.05; p = 0.04). No association between CLT and recurrence risk was found in men. Hypofibrinolysis as assessed by CLT confers a moderate increase in the risk of recurrent VTE. A weak association between CLT and risk of recurrence was found in women only.

  16. Prediction of recurrent venous thromboembolism by clot lysis time: a prospective cohort study.

    Directory of Open Access Journals (Sweden)

    Ludwig Traby

    Full Text Available Venous thromboembolism (VTE is a chronic disease, which tends to recur. Whether an abnormal fibrinolytic system is associated with an increased risk of VTE is unclear. We assessed the relationship between fibrinolytic capacity (reflected by clot lysis time [CLT] and risk of recurrent VTE. We followed 704 patients (378 women; mean age 48 yrs with a first unprovoked VTE for an average of 46 months after anticoagulation withdrawal. Patients with natural coagulation inhibitor deficiency, lupus anticoagulant, cancer, homozygosity for factor V Leiden or prothrombin mutation, or requirement for indefinite anticoagulation were excluded. Study endpoint was symptomatic recurrent VTE. For measurement of CLT, a tissue factor-induced clot was lysed by adding tissue-type plasminogen activator. Time between clot formation and lysis was determined by measuring the turbidity. 135 (19% patients had recurrent VTE. For each increase in CLT of 10 minutes, the crude relative risk (RR of recurrence was 1.13 (95% CI 1.02-1.25; p = 0.02 and was 1.08 (95% CI 0.98-1.20; p = 0.13 after adjustment for age and sex. For women only, the adjusted RR was 1.14 (95% CI, 0.91-1.42, p = 0.22 for each increase in CLT of 10 minutes. CLT values in the 4(th quartile of the female patient population, as compared to values in the 1(st quartile, conferred a risk of recurrence of 3.28 (95% CI, 1.07-10.05; p = 0.04. No association between CLT and recurrence risk was found in men. Hypofibrinolysis as assessed by CLT confers a moderate increase in the risk of recurrent VTE. A weak association between CLT and risk of recurrence was found in women only.

  17. Biological variation in tPA-induced plasma clot lysis time.

    Science.gov (United States)

    Talens, Simone; Malfliet, Joyce J M C; Rudež, Goran; Spronk, Henri M H; Janssen, Nicole A H; Meijer, Piet; Kluft, Cornelis; de Maat, Moniek P M; Rijken, Dingeman C

    2012-10-01

    Hypofibrinolysis is a risk factor for venous and arterial thrombosis, and can be assessed by using a turbidimetric tPA-induced clot lysis time (CLT) assay. Biological variation in clot lysis time may affect the interpretation and usefulness of CLT as a risk factor for thrombosis. Sufficient information about assay variation and biological variation in CLT is not yet available. Thus, this study aimed to determine the analytical, within-subject and between-subject variation in CLT. We collected blood samples from 40 healthy individuals throughout a period of one year (average 11.8 visits) and determined the CLT of each plasma sample in duplicate. The mean (± SD) CLT was 83.8 (± 11.1) minutes. The coefficients of variation for total variation, analytical variation, within-subject variation and between-subject variation were 13.4%, 2.6%, 8.2% and 10.2%, respectively. One measurement can estimate the CLT that does not deviate more than 20% from its true value. The contribution of analytical variation to the within-subject variation was 5.0%, the index of individuality was 0.84 and the reference change value was 23.8%. The CLT was longer in the morning compared to the afternoon and was slightly longer in older individuals (> 40 years) compared to younger (≤40 years) individuals. There was no seasonal variation in CLT and no association with air pollution. CLT correlated weakly with fibrinogen, C-reactive protein, prothrombin time and thrombin generation. This study provides insight into the biological variation of CLT, which can be used in future studies testing CLT as a potential risk factor for thrombosis.

  18. Estenosis traqueal: Estudio preliminar

    Directory of Open Access Journals (Sweden)

    Rafael Pinilla González

    2000-12-01

    Full Text Available Se realizó un estudio retrospectivo y descriptivo con la utilización de los expedientes clínicos de 5 pacientes con estenosis traqueal, mayoritariamente como consecuencia de intubación prolongada, y en el que se tuvo en cuenta aspectos relacionados con el diagnóstico, el tratamiento y las complicaciones. Existió un predominio del sexo femenino y edad media de 44,6 años. La intubación prolongada de origen diverso fue la causa de la estenosis en 4 de los 5 pacientes y en 1 fue posterior a la reparación de lesión traqueal yatrógena. La instalación de la estenosis tuvo lugar entre 8 días y 3 meses. La tomografía lineal en 3 pacientes y en 2 la radiografía simple de tráquea anteroposterior y lateral verificaron el diagnóstico. La endoscopia traqueobronquial preoperatoria fue de utilidad en la caracterización de la estenosis. En todos los pacientes se realizó tratamiento quirúrgico al resecar el área estenótica con posterior anastomosis terminoterminal y fijación del cuello en flexión durante 8 días como promedio. Dos pacientes mostraron granulomas en el nivel de la línea de sutura, los cuales fueron tratados por vacuolización con láser. No se reportó mortalidad en la muestra estudiadaA retrospective and descriptive study was conducted by using the medical histories of 5 patients with tracheal stenosis, mostly as a result of prolongued intubation. Aspects connected with the diagnosis, treatment and complications were taken into account. It was observed a predominance of females and an average age of 44.6. Prolongued intubation of different origin was the cause of stenosis in 4 of the 5 patients, whereas in the other it occurred after the reparation of the tracheal and athrogenous lesion. The installation of stenosis took place between 8 days and 3 months. Lineal tomography in 3 patients and anteroposterior and lateral simple X-ray of the trachea in 2 confirmed the diagnosis. Preoperative tracheobronchial endoscopy was

  19. Estudios Experimentales 2 Parte: Estudios Cuasi-Experimentales

    OpenAIRE

    Manterola, Carlos; Otzen, Tamara

    2015-01-01

    Los estudios experimentales, se caracterizan por la valoración del efecto de una o más intervenciones, habitualmente de forma comparativa con otra intervención, o un placebo; y el carácter prospectivo, de la recolección de datos y seguimiento. Agrupados bajo esta denominación, existe una diversidad de diseños, entre los que se encuentran los estudios cuasi-experimentales (ECE), que se caracterizan especialmente por la ausencia de asignación aleatoria. El objetivo de este manuscrito, es report...

  20. Role of adrenal hormones and prostaglandins in the control of mouse thymocytes lysis.

    Science.gov (United States)

    Durant, S; Seillan, C; Duval, D; Homo-Delarche, F

    1984-01-01

    The cytolytic actions of glucocorticoids and of agents increasing cyclic AMP were studied in vitro in thymocyte suspensions isolated from adrenalectomized or hydrocortisone-treated mice. Although considered as corticoresistant cells, the thymocytes isolated from hydrocortisone-treated mice were lysed to the same extent although more slowly in vitro by dexamethasone than whole thymocyte populations (i.e. corticosensitive cells). Moreover, these two cell populations were shown to contain comparable amounts of glucocorticoid receptors and to be almost equally sensitive to the metabolic effects of glucocorticoids when measured by inhibition of RNA and DNA synthesis. Studies performed with corticosensitive cells showed that prostaglandin E2, isoproterenol and dibutyrilcyclic AMP were also able to induce cell lysis and that, isoproterenol and dexamethasone exerted additive cytolytic action in vitro. In vivo experiments showed also an additive effect of steroids and isoproterenol on thymus atrophy. In contrast, cells isolated from hydrocortisone-treated animals were not sensitive to the cytotoxic action of prostaglandin E2, isoproterenol and dibutyril cyclic AMP. This difference between the two populations was not associated with any difference in the responsiveness of adenylate cyclase as determined following isoproterenol-induced accumulation of cyclic AMP. The cytolytic action of dexamethasone but also that of prostaglandin E2 and isoproterenol, could be blocked in the presence of cycloheximide, an inhibitor of protein synthesis, thus suggesting that glucocorticoids and agents increasing cyclic AMP control the synthesis of some proteins involved in the triggering of cell lysis. Among the hypotheses proposed to explain the differences between in vitro and in vivo sensitivity of lymphoid cell to glucocorticoids, it was suggested that the drug may in vivo indirectly control the viability or the proliferation of thymocytes through the release of other mediators. We have

  1. Features of target cell lysis by class I and class II MHC restricted cytolytic T lymphocytes

    International Nuclear Information System (INIS)

    Maimone, M.M.; Morrison, L.A.; Braciale, V.L.; Braciale, T.J.

    1986-01-01

    The lytic activity of influenza virus-specific muvine cytolytic T lymphocyte (CTL) clones that are restricted by either H-2K/D (class I) or H-2I (class II) major histocompatibility (MHC) locus products was compared on an influenza virus-infected target cell expressing both K/D and I locus products. With the use of two in vitro measurements of cytotoxicity, conventional 51 Cr release, and detergent-releasable radiolabeled DNA (as a measure of nuclear disintegration in the early post-lethal hit period), the authors found no difference between class I and class II MHC-restricted CTL in the kinetics of target cell destruction. In addition, class II MHC-restricted antiviral CTL failed to show any lysis of radiolabeled bystander cells. Killing of labeled specific targets by these class II MHC-restricted CTL was also efficiently inhibited by unlabeled specific competitor cells in a cold target inhibition assay. In sum, these data suggest that class I and class II MHC-restricted CTL mediate target cell destruction by an essentially similar direct mechanism

  2. Plasma clot lysis time and its association with cardiovascular risk factors in black Africans.

    Directory of Open Access Journals (Sweden)

    Zelda de Lange

    Full Text Available Studies in populations of European descent show longer plasma clot lysis times (CLT in patients with cardiovascular disease (CVD than in controls. No data are available on the association between CVD risk factors and fibrinolytic potential in black Africans, a group undergoing rapid urbanisation with increased CVD prevalence. We investigated associations between known CVD risk factors and CLT in black Africans and whether CLTs differ between rural and urban participants in light of differences in CVD risk.Data from 1000 rural and 1000 urban apparently healthy black South Africans (35-60 years were cross-sectionally analysed.Increased PAI-1(act, BMI, HbA1c, triglycerides, the metabolic syndrome, fibrinogen concentration, CRP, female sex and positive HIV status were associated with increased CLTs, while habitual alcohol consumption associated with decreased CLT. No differences in CLT were found between age and smoking categories, contraceptive use or hyper- and normotensive participants. Urban women had longer CLT than rural women while no differences were observed for men.CLT was associated with many known CVD risk factors in black Africans. Differences were however observed, compared to data from populations of European descent available in the literature, suggesting possible ethnic differences. The effect of urbanisation on CLT is influenced by traditional CVD risk factors and their prevalence in urban and rural communities.

  3. Floor of the nose mucosa lysis and labial abscess caused by a bee sting.

    Science.gov (United States)

    Alemán Navas, Ramón Manuel; Martínez Mendoza, María Guadalupe; Herrera, Henry; Herrera, Helen Piccolo de

    2009-01-01

    Hymenoptera order includes bees, which have a stinging apparatus at the tail capable of delivering venom to the affected tissues. Myocardial infarction, acute renal failure, Necrotizing fasciitis, fatal infection and hemifacial asymmetry, are some of the unusual reactions reported following hymenoptera stings. This paper reports a case of bee sting in the right floor of the nose that mimicked an odontogenic infection affecting the upper lip, canine space and nasal cavity such as in cases of infection secondary to pulpal or periodontal pathology of the anterior teeth. After a thorough clinical and radiographic examination, odontogenic infection was discarded and the diagnosis of floor of the nose mucosal lysis and lip abscess secondary to a bee sting was made. This case was successfully managed with adequate incision, drainage and antibiotics without any further complication. There are several reports of unusual reactions following hymenoptera stings. However, just a few of them referred to infections of local reactions and none of them related to the anatomic location affected in the patient of the present case. Early diagnosis and treatment prevented infection dissemination and the likelihood of tissue necrosis as in previously reported cases of Necrotizing fasciitis.

  4. Characterization of cell lysis in Pseudomonas putida induced upon expression of heterologous killing genes

    DEFF Research Database (Denmark)

    Ronchel, M.C.; Molina, L.; Witte, A.

    1998-01-01

    Active biological containment systems are based on the controlled expression of killing genes. These systems are of interest for the Pseudomonadaceae because of the potential applications of these microbes as bioremediation agents and biopesticides, The physiological effects that lead to cell dea...... protein was the killing agent. In both cases, cell death occurred as a result of impaired respiration, altered membrane permeability, and the release of some cytoplasmic contents to the extracellular medium.......Active biological containment systems are based on the controlled expression of killing genes. These systems are of interest for the Pseudomonadaceae because of the potential applications of these microbes as bioremediation agents and biopesticides, The physiological effects that lead to cell death......, respectively. Expression of the killing genes is controlled by the LacI protein, whose expression is initiated from the XylS-dependent Pm promoter. Under induced conditions, killing of P. putida CMC12 cells mediated by phi X174 lysis protein E was faster than that observed for P. putida CMC4, for which the Gef...

  5. Large-scale clinical comparison of the lysis-centrifugation and radiometric systems for blood culture

    International Nuclear Information System (INIS)

    Brannon, P.; Kiehn, T.E.

    1985-01-01

    The Isolator 10 lysis-centrifugation blood culture system (E. I. du Pont de Nemours and Co., Inc., Wilmington, Del.) was compared with the BACTEC radiometric method (Johnston Laboratories, Inc., Towson, Md.) with 6B and 7D broth media for the recovery of bacteria and yeasts. From 11,000 blood cultures, 1,174 clinically significant organisms were isolated. The Isolator system recovered significantly more total organisms, members of the family Enterobacteriaceae, Staphylococcus spp., and yeasts. The BACTEC system recovered significantly more Pseudomonas spp., Streptococcus spp., and anaerobes. Of the Isolator colony counts, 87% measured less than 11 CFU/ml of blood. Organisms, on an average, were detected the same day from each of the two culture systems. Only 13 of the 975 BACTEC isolates (0.01%) were recovered by subculture of growth-index-negative bottles, and 12 of the 13 were detected in another broth blood culture taken within 24 h. Contaminants were recovered from 4.8% of the Isolator 10 and 2.3% of the BACTEC cultures

  6. Plasma clot lysis time and its association with cardiovascular risk factors in black Africans.

    Science.gov (United States)

    de Lange, Zelda; Pieters, Marlien; Jerling, Johann C; Kruger, Annamarie; Rijken, Dingeman C

    2012-01-01

    Studies in populations of European descent show longer plasma clot lysis times (CLT) in patients with cardiovascular disease (CVD) than in controls. No data are available on the association between CVD risk factors and fibrinolytic potential in black Africans, a group undergoing rapid urbanisation with increased CVD prevalence. We investigated associations between known CVD risk factors and CLT in black Africans and whether CLTs differ between rural and urban participants in light of differences in CVD risk.Data from 1000 rural and 1000 urban apparently healthy black South Africans (35-60 years) were cross-sectionally analysed.Increased PAI-1(act), BMI, HbA1c, triglycerides, the metabolic syndrome, fibrinogen concentration, CRP, female sex and positive HIV status were associated with increased CLTs, while habitual alcohol consumption associated with decreased CLT. No differences in CLT were found between age and smoking categories, contraceptive use or hyper- and normotensive participants. Urban women had longer CLT than rural women while no differences were observed for men.CLT was associated with many known CVD risk factors in black Africans. Differences were however observed, compared to data from populations of European descent available in the literature, suggesting possible ethnic differences. The effect of urbanisation on CLT is influenced by traditional CVD risk factors and their prevalence in urban and rural communities.

  7. Tumour lysis syndrome: A rare acute presentation of locally advanced testicular cancer – Case report and review of literature

    Directory of Open Access Journals (Sweden)

    Marcus Chow

    2016-01-01

    Full Text Available Tumour lysis syndrome (TLS is a potentially fatal complication of malignancy or its treatment. This uncommon syndrome comprises laboratory findings of hyperuricaemia, hypocalcaemia, hyperkalaemia and hyperphosphataemia. A literature search revealed a total of eight patients, with testicular cancer, who had TLS. All these patients had metastatic disease. We present a unique case of a 47-year-old gentleman we saw in clinic, who presented with a rapidly growing right groin mass and acute breathlessness, and discuss the diagnosis and management of TLS. TLS is extremely rare in testicular cancer but necessitates the awareness of urologists. TLS can occur spontaneously in testicular malignancy. Cell lysis in a rapidly proliferating germ cell tumour is a possible mechanism. The prompt identification and institution of management for TLS is crucial to improve clinical outcomes.

  8. Study of a novel cell lysis method with titanium dioxide for Lab-on-a-Chip devices.

    Science.gov (United States)

    Wan, Weijie; Yeow, John T W

    2011-06-01

    In this paper, a novel method is proposed and demonstrated to be able to lyse gram-negative (E. coli) bacteria cells for Lab-on-a-Chip applications. The proposed method incorporates using titanium dioxide particles as photocatalysts and a miniaturized UV LED array as an excitation light source to perform cell lysis on microchips. The experimental result demonstrates the feasibility of the proposed prototype device. The working device suggests an inexpensive, easy to be fabricated and effective way for microchip cell lysis. The miniaturized UV LED array and the microchip with a reaction chamber can be easily integrated with other functional components to form a customized whole Lab-on-a-Chip system.

  9. Sulfolobus turreted icosahedral virus c92 protein responsible for the formation of pyramid-like cellular lysis structures.

    Science.gov (United States)

    Snyder, Jamie C; Brumfield, Susan K; Peng, Nan; She, Qunxin; Young, Mark J

    2011-07-01

    Host cells infected by Sulfolobus turreted icosahedral virus (STIV) have been shown to produce unusual pyramid-like structures on the cell surface. These structures represent a virus-induced lysis mechanism that is present in Archaea and appears to be distinct from the holin/endolysin system described for DNA bacteriophages. This study investigated the STIV gene products required for pyramid formation in its host Sulfolobus solfataricus. Overexpression of STIV open reading frame (ORF) c92 in S. solfataricus alone is sufficient to produce the pyramid-like lysis structures in cells. Gene disruption of c92 within STIV demonstrates that c92 is an essential protein for virus replication. Immunolocalization of c92 shows that the protein is localized to the cellular membranes forming the pyramid-like structures.

  10. Fed-Batch Production of Bacterial Ghosts Using Dielectric Spectroscopy for Dynamic Process Control

    Directory of Open Access Journals (Sweden)

    Andrea Meitz

    2016-03-01

    Full Text Available The Bacterial Ghost (BG platform technology evolved from a microbiological expression system incorporating the ϕX174 lysis gene E. E-lysis generates empty but structurally intact cell envelopes (BGs from Gram-negative bacteria which have been suggested as candidate vaccines, immunotherapeutic agents or drug delivery vehicles. E-lysis is a highly dynamic and complex biological process that puts exceptional demands towards process understanding and control. The development of a both economic and robust fed-batch production process for BGs required a toolset capable of dealing with rapidly changing concentrations of viable biomass during the E-lysis phase. This challenge was addressed using a transfer function combining dielectric spectroscopy and soft-sensor based biomass estimation for monitoring the rapid decline of viable biomass during the E-lysis phase. The transfer function was implemented to a feed-controller, which followed the permittivity signal closely and was capable of maintaining a constant specific substrate uptake rate during lysis phase. With the described toolset, we were able to increase the yield of BG production processes by a factor of 8–10 when compared to currently used batch procedures reaching lysis efficiencies >98%. This provides elevated potentials for commercial application of the Bacterial Ghost platform technology.

  11. Study of the flooding and dehydration processes of a PEM fuel cell using the EIS technique; Estudio de los procesos de inundacion y deshidratacion en una celda de combustible tipo PEM mediante la tecnica EIS

    Energy Technology Data Exchange (ETDEWEB)

    Loyola-Morales, F.; Cano-Castillo, U. [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)]. E-mail: feloyola@yahoo.com.mx

    2009-09-15

    In this work, a study was conducted of the flooding and dehydration processes of a PEM fuel cell using the EIS technique. The experiments were conducted in a 50 cm{sup 2} cell. The gradual flooding of the system was induced by operating the cell at a potential of 0.3 V and maintaining the gas outlet closed (that is, stoichiometry of 1 for the anode (H{sub 2}) and the cathode (O{sub 2})) to enable the water produced by the reaction to accumulate inside. The gradual dehydration was induced by operating the cell at a potential of 0.3V and establishing a oxidized gas flow at a stoichiometry of 4. EIS tests were applied throughout both processes. The results showed that the EIS technique is highly sensitive for the analysis of the different degrees of the flooding processes by monitoring variations in the imaginary components of total impedance (Z{sup )} or the phase angle ({theta}). For low degrees of flooding, the technique had good sensitivity, between 1 and 6 Hz, while at high degrees of flooding the technique's greatest sensitivity was limited to a range between 1 and 2 Hz. In the case of the dehydration process of the system, the results showed that this type of process can be analyzed for variations in the value of the real component (Z{sup '}) as well as for the imaginary component of total impedance and variations in the phase angle. The analysis of dehydration with Z{sup '} was possible at a rather wide range, from 100 to 1000 Hz; with Z{sup }or {theta} it was only possible at a range of 20 to 200 Hz. [Spanish] En el presente trabajo, se llevo a cabo el estudio de los procesos de inundacion y deshidratacion de una celda de combustible tipo PEM mediante la tecnica EIS. Los experimentos fueron realizados en una celda de 50 cm{sup 2}. La inundacion gradual del sistema se indujo operando la celda a un potencial de 0.3 V de celda y manteniendo la salida de gases cerrada (i. e. estequiometria de 1 tanto en anodo (H{sub 2}) como en catodo (O{sub 2

  12. Regulated programmed lysis of recombinant Salmonella in host tissues to release protective antigens and confer biological containment

    OpenAIRE

    Kong, Wei; Wanda, Soo-Young; Zhang, Xin; Bollen, Wendy; Tinge, Steven A.; Roland, Kenneth L.; Curtiss, Roy

    2008-01-01

    We have devised and constructed a biological containment system designed to cause programmed bacterial cell lysis with no survivors. We have validated this system, using Salmonella enterica serovar Typhimurium vaccines for antigen delivery after colonization of host lymphoid tissues. The system is composed of two parts. The first component is Salmonella typhimurium strain χ8937, with deletions of asdA and arabinose-regulated expression of murA, two genes required for peptidoglycan synthesis a...

  13. A super-family of transcriptional activators regulates bacteriophage packaging and lysis in Gram-positive bacteria

    Science.gov (United States)

    Quiles-Puchalt, Nuria; Tormo-Más, María Ángeles; Campoy, Susana; Toledo-Arana, Alejandro; Monedero, Vicente; Lasa, Íñigo; Novick, Richard P.; Christie, Gail E.; Penadés, José R.

    2013-01-01

    The propagation of bacteriophages and other mobile genetic elements requires exploitation of the phage mechanisms involved in virion assembly and DNA packaging. Here, we identified and characterized four different families of phage-encoded proteins that function as activators required for transcription of the late operons (morphogenetic and lysis genes) in a large group of phages infecting Gram-positive bacteria. These regulators constitute a super-family of proteins, here named late transcriptional regulators (Ltr), which share common structural, biochemical and functional characteristics and are unique to this group of phages. They are all small basic proteins, encoded by genes present at the end of the early gene cluster in their respective phage genomes and expressed under cI repressor control. To control expression of the late operon, the Ltr proteins bind to a DNA repeat region situated upstream of the terS gene, activating its transcription. This involves the C-terminal part of the Ltr proteins, which control specificity for the DNA repeat region. Finally, we show that the Ltr proteins are the only phage-encoded proteins required for the activation of the packaging and lysis modules. In summary, we provide evidence that phage packaging and lysis is a conserved mechanism in Siphoviridae infecting a wide variety of Gram-positive bacteria. PMID:23771138

  14. RNA integrity as a quality indicator during the first steps of RNP purifications : A comparison of yeast lysis methods

    Directory of Open Access Journals (Sweden)

    Jansen Ralf-Peter

    2004-10-01

    Full Text Available Abstract Background The completion of several genome-sequencing projects has increased our need to assign functions to newly identified genes. The presence of a specific protein domain has been used as the determinant for suggesting a function for these new genes. In the case of proteins that are predicted to interact with mRNA, most RNAs bound by these proteins are still unknown. In yeast, several protocols for the identification of protein-protein interactions in high-throughput analyses have been developed during the last years leading to an increased understanding of cellular proteomics. If any of these protocols or similar approaches shall be used for the identification of mRNA-protein complexes, the integrity of mRNA is a critical factor. Results We compared the effect of different lysis protocols on RNA integrity. We report dramatic differences in RNA stability depending on the method used for yeast cell lysis. Glass bead milling and French Press lead to degraded mRNAs even in the presence of RNase inhibitors. Thus, they are not suitable to purify intact mRNP complexes or to identify specific mRNAs bound to proteins. Conclusion We suggest a novel protocol, grinding deep-frozen cells, for the preparation of protein extracts that contain intact RNAs, as lysis method for the purification of mRNA-protein complexes from yeast cells.

  15. Determining Human Clot Lysis Time (in vitro with Plasminogen/Plasmin from Four Species (Human, Bovine, Goat, and Swine

    Directory of Open Access Journals (Sweden)

    Omaira Cañas Bermúdez

    2015-05-01

    Full Text Available Cardiovascular disease is the leading cause of death worldwide, including failures in the plasminogen/plasmin system which is an important factor in poor lysis of blood clots. This article studies the fibrinolytic system in four species of mammals, and it identifies human plasminogen with highest thrombolysis efficiency. It examines plasminogen from four species (human, bovine, goat, and swine and identifies the most efficient one in human clot lysis in vitro. All plasminogens were identically purified by affinity chromatography. Human fibrinogen was purified by fractionation with ethanol. The purification of both plasminogen and fibrinogen was characterized by one-dimensional SDS-PAGE (10%. Human clot formation in vitro and its dissolution by plasminogen/plasmin consisted of determining lysis time from clot formation to its dilution. Purification of proteins showed greater than 95% purity, human plasminogen showed greater ability to lyse clot than animal plasminogen. The article concludes that human plasminogen/plasmin has the greatest catalysis and efficiency, as it dissolves human clot up to three times faster than that of irrational species.

  16. [Ultrasound dynamics lysis apex thrombus as an objective criterion of effectiveness of anticoagulation therapy in venous thrombosis].

    Science.gov (United States)

    Kalinin, R E; Suchkov, I A; Pshennikov, A S; Agapov, A B

    2016-01-01

    To assess the effectiveness of anticoagulant therapy (ACT) for the treatment of patients with deep venous thrombosis (DVT) of the lower extremities. The study considered ultrasonic characteristics of lysis of the proximal part of thrombus: localization and nature of venous thrombosis, the length and diameter of the proximal floating part of the thrombus, and duration of the venous thrombosis. Depending on the ACT options patients were divided into 3 groups: Group 1 (18 patients) received rivaroxaban, group 2 (19 patients) received enoxaparin sodium with subsequent transition to warfarin, and 3 group (19 patietns) received enoxaparin sodium, followed by administration of rivaroxaban. Treatment with rivaroxaban was preferable over standard ACT with enoxaparin/warfarin with regards to the lysis of thrombus when duration of thrombosis did not exceed 10 days. In 10.5% of patients who received warfarin flotation of thrombi remained for 14 days; the length of the floating part of the thrombi did not exceed 3 cm. Such circumstances and inability to reach a therapeutic INR value required cava filter placement. Treatment with enoxaparin sodium followed by the administration of rivaroxaban was found to be the most efficient ACT regimen as there was no negative dynamics of ultrasound characteristics of lysis of thrombi at any duration of the disease.

  17. Comparison of clot lysis activity and biochemical properties of originator tenecteplase (Metalyse® with those of an alleged biosimilar

    Directory of Open Access Journals (Sweden)

    Werner eKliche

    2014-02-01

    Full Text Available The bioengineered tissue plasminogen activator tenecteplase is an important treatment modality of acute myocardial infarction recommended by international guidelines. Following introduction of originator tenecteplase (brand names Metalyse® and TNKase®, a ‘biosimilar’ tenecteplase became available for commercial use in India under the brand name Elaxim® in the absence of Indian biosimilar guidelines which came into force from September 15th, 2012. Based on a report of biochemical and fibrinolytical differences between Metalyse and Elaxim, we have systematically compared them in a range of routine quality testing assays. As compared to Metalyse, Elaxim exhibited less clot lysis activity and contained less of the two-chain form of tenecteplase. Even upon full in vitro conversion to the two-chain form Elaxim exhibited less clot lysis activity. This was linked to differences in sialic acid content and glycosylation pattern with Elaxim exhibiting less bi- and more tetra-antennary glycosylation, leading to different charge heterogeneity profile. Regarding purity, Elaxim contained more tenecteplase aggregates and, in contrast to Metalyse, considerable amounts of Chinese hamster ovary cell protein. Taken together these data demonstrate that Metalyse and Elaxim differ considerably in clot lysis activity and biochemical properties. These data question whether Elaxim indeed can be considered a ‘biosimilar’ of Metalyse, i.e. whether and to which extent the clinical efficacy and safety properties of Metalyse can be extrapolated to Elaxim in the absence of comparative clinical data.

  18. Sulfolobus Turreted Icosahedral Virus c92 Protein Responsible for the Formation of Pyramid-Like Cellular Lysis Structures

    DEFF Research Database (Denmark)

    Snyder, Jamie C; Brumfield, Susan K; Peng, Nan

    2011-01-01

    Host cells infected by Sulfolobus turreted icosahedral virus (STIV) have been shown to produce unusual pyramid-like structures on the cell surface. These structures represent a virus-induced lysis mechanism that is present in Archaea and appears to be distinct from the holin/endolysin system desc...... disruption of c92 within STIV demonstrates that c92 is an essential protein for virus replication. Immunolocalization of c92 shows that the protein is localized to the cellular membranes forming the pyramid-like structures.......Host cells infected by Sulfolobus turreted icosahedral virus (STIV) have been shown to produce unusual pyramid-like structures on the cell surface. These structures represent a virus-induced lysis mechanism that is present in Archaea and appears to be distinct from the holin/endolysin system...... described for DNA bacteriophages. This study investigated the STIV gene products required for pyramid formation in its host Sulfolobus solfataricus. Overexpression of STIV open reading frame (ORF) c92 in S. solfataricus alone is sufficient to produce the pyramid-like lysis structures in cells. Gene...

  19. Relative efficacy of the argon green, argon blue-green, and krypton red lasers for 10-0 nylon subconjunctival laser suture lysis.

    Science.gov (United States)

    Mudgil, A V; To, K W; Balachandran, R M; Janigian, R H; Tsiaras, W G

    1999-01-01

    To determine the optimal wavelength for subconjunctival laser suture lysis. 130 black monofilament 10-0 nylon sutures were sewn subconjunctivally into the bare sclera of enucleated rabbit globes. The lowest energy levels facilitating laser suture lysis were determined for the argon green (514.5 NM), argon blue-green (488.0 NM, 514.5 NM), and krypton red (647.1 NM) wavelengths. In addition, absorption spectroscopy was performed on the suture material and conjunctiva using the Perkin Elmer W/VIS Lambda 2 spectrometer. Krypton red produced the fewest buttonhole defects, and it was also the most efficient energy source for suture lysis (P = 0.0001) under nontenectomized conjunctiva. Absorbance spectra studies revealed peak absorbance at 628 NM for the 10-0 nylon suture material. Based on animal and absorption spectroscopy studies, krypton red may be a safer and more efficient wavelength for subconjunctival laser suture lysis.

  20. Technical study of the CO{sub 2} capture process with monoethanolamine for a thermoelectric plant; Estudio tecnico del proceso de captura de CO{sub 2} con monoetanolamina para una planta termoelectrica

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez Diaz, Abigail; Franco Nava, Jose Manuel; Peralta Martinez, Maria Vita; Gonzalez Santalo, Jose Miguel [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico); Franco Lopez, Rogelio; Carreon Silva, Ramon [Comision Federal de Electricidad (Mexico)

    2010-07-01

    Within the frame of the Special Program of Climatic Change (PECC) emitted by the Federal Government, the study of the process of CO{sub 2} capture is carried out post-combustion for application in generating power stations, as a possible alternative to reduce the CO{sub 2} emissions in Mexico. The simulation of the process of CO{sub 2} capture of gases generated by a thermoelectric power plant of 350 MW was carried out, that would use coal as fuel, in the processes simulator ASPEN HYSYS. For this process two columns are required: one of absorption, in which the gases and the solvent enter, that in this case were a solution of monoethanolamine (MEA) at 30%. MEA reacts with the CO{sub 2} contained in gases, retaining it, so that the remaining gases that are emitted to the atmosphere contain no longer MEA and the captured CO{sub 2} passes to the second column where these two components are separated, using thermal energy to regenerate the MEA releasing the CO{sub 2}. The released CO{sub 2} leaves though the upper part of the column and the MEA recovered that is reused in the absorption column, through the bottom. The CO{sub 2} concentration in gases of the combustion is of 14.54% by volume. The simulation was realized defining a capture efficiency of 90%, which threw a thermal power consumption to regenerate MEA of 4.75 GJt/ton CO{sub 2} that would be provided by the thermoelectric power station. Considering a capacity of 280 ton/h (by train) of the gas to be treated the height of the desertion and absorption columns was determined, as well as the MEA solution flow. [Spanish] Dentro del marco del Programa Especial de Cambio Climatico (PECC) emitido por el Gobierno Federal, se lleva a cabo el estudio del proceso de captura de CO{sub 2} poscombustion para aplicacion en centrales generadoras, como una posible alternativa para reducir las emisiones de CO{sub 2} en Mexico. Se llevo a cabo la simulacion del proceso de captura de CO{sub 2} de los gases generados por una

  1. Comparison of the lysis centrifugation method with the conventional blood culture method in cases of sepsis in a tertiary care hospital.

    Science.gov (United States)

    Parikh, Harshal R; De, Anuradha S; Baveja, Sujata M

    2012-07-01

    Physicians and microbiologists have long recognized that the presence of living microorganisms in the blood of a patient carries with it considerable morbidity and mortality. Hence, blood cultures have become critically important and frequently performed test in clinical microbiology laboratories for diagnosis of sepsis. To compare the conventional blood culture method with the lysis centrifugation method in cases of sepsis. Two hundred nonduplicate blood cultures from cases of sepsis were analyzed using two blood culture methods concurrently for recovery of bacteria from patients diagnosed clinically with sepsis - the conventional blood culture method using trypticase soy broth and the lysis centrifugation method using saponin by centrifuging at 3000 g for 30 minutes. Overall bacteria recovered from 200 blood cultures were 17.5%. The conventional blood culture method had a higher yield of organisms, especially Gram positive cocci. The lysis centrifugation method was comparable with the former method with respect to Gram negative bacilli. The sensitivity of lysis centrifugation method in comparison to conventional blood culture method was 49.75% in this study, specificity was 98.21% and diagnostic accuracy was 89.5%. In almost every instance, the time required for detection of the growth was earlier by lysis centrifugation method, which was statistically significant. Contamination by lysis centrifugation was minimal, while that by conventional method was high. Time to growth by the lysis centrifugation method was highly significant (P value 0.000) as compared to time to growth by the conventional blood culture method. For the diagnosis of sepsis, combination of the lysis centrifugation method and the conventional blood culture method with trypticase soy broth or biphasic media is advocable, in order to achieve faster recovery and a better yield of microorganisms.

  2. Virus-specific HLA-restricted lysis of herpes simplex virus-infected human monocytes and macrophages mediated by cytotoxic T lymphocytes

    Energy Technology Data Exchange (ETDEWEB)

    Torpey, D.J. III

    1987-01-01

    Freshly-isolated peripheral blood human monocytes and 5 day in vitro cultured macrophages were infected with herpes simplex virus type 1 (HSV-1), labeled with /sup 51/Cr, and used as target cells in a 12-14 hour cell-mediated cytotoxicity assay. Mononuclear leukocytes (MNL) from HSV-1 non-immune individuals, whether unstimulated or stimulated with HSV-1 antigen, did not mediate significant lysis of either target cell. HSV-immune MNL, both freshly-isolated and cultured for 5 days without antigen, demonstrated only low levels of natural killer (NK) cell-mediate lysis. MNL from HSV-immune individuals incubated for 5 days in vitro with HSV-1 antigen mediated significant virus-specific lysis of both target cells. Mean virus-specific lysis of autologous monocytes was 8.5(/+-/2.0)% compared to a three-fold greater virus-specific lysis of autologous macrophages. Greater than 70% of this lytic activity was mediated by Leu-11-negative, T3-positive cytotoxic T lymphocytes (CTL). Allogeneic target cells lacking a common HLA determinant were not significantly lysed while T8-positive CTL mediated infrequent lysis of target cells sharing a common HLA-A and/or HLA-B determinant. T4-positive lymphocytes were demonstrated to be the predominant cell mediating lysis of autologous target cells and allogeneic target cells sharing both HLA-A and/or HLA-B plus HLA-DR determinants with the CTL; the T4-positive cell was the sole CTL mediator of lysis of allogeneic target cells having a common HLA-DR determinant.

  3. Virus-specific HLA-restricted lysis of herpes simplex virus-infected human monocytes and macrophages mediated by cytotoxic T lymphocytes

    International Nuclear Information System (INIS)

    Torpey, D.J. III.

    1987-01-01

    Freshly-isolated peripheral blood human monocytes and 5 day in vitro cultured macrophages were infected with herpes simplex virus type 1 (HSV-1), labeled with 51 Cr, and used as target cells in a 12-14 hour cell-mediated cytotoxicity assay. Mononuclear leukocytes (MNL) from HSV-1 non-immune individuals, whether unstimulated or stimulated with HSV-1 antigen, did not mediate significant lysis of either target cell. HSV-immune MNL, both freshly-isolated and cultured for 5 days without antigen, demonstrated only low levels of natural killer (NK) cell-mediate lysis. MNL from HSV-immune individuals incubated for 5 days in vitro with HSV-1 antigen mediated significant virus-specific lysis of both target cells. Mean virus-specific lysis of autologous monocytes was 8.5(/+-/2.0)% compared to a three-fold greater virus-specific lysis of autologous macrophages. Greater than 70% of this lytic activity was mediated by Leu-11-negative, T3-positive cytotoxic T lymphocytes (CTL). Allogeneic target cells lacking a common HLA determinant were not significantly lysed while T8-positive CTL mediated infrequent lysis of target cells sharing a common HLA-A and/or HLA-B determinant. T4-positive lymphocytes were demonstrated to be the predominant cell mediating lysis of autologous target cells and allogeneic target cells sharing both HLA-A and/or HLA-B plus HLA-DR determinants with the CTL; the T4-positive cell was the sole CTL mediator of lysis of allogeneic target cells having a common HLA-DR determinant

  4. Local lysis with Alteplase for the treatment of acute embolic leg ischemia following the use of the Duett trademark closure device: preliminary results

    International Nuclear Information System (INIS)

    Schuermann, K.; Buecker, A.; Wingen, M.; Tacke, J.; Wein, B.; Guenther, R.W.; Janssens, U.

    2004-01-01

    Purpose: To analyze retrospectively the result of the alteplase lysis therapy of embolic complications following the use of the Duett closure device. Methods and Materials: For 3.5 years, the Duett closure device was used in 1,398 angiographies to close the femoral puncture site. The Duett device consists of a balloon and a liquid procoagulant containing collagen and thrombin, which is injected into the puncture tract under endovascular balloon protection of the arterial puncture site. In 9 patients (0.64%), the procoagulant was incidentally injected into the femoral artery causing acute leg ischemia. Eight patients received local lysis therapy with alteplase via a contralateral femoral access. One patient underwent surgery. On average, 21 mg alteplase (4-35 mg) were administered within 14 h (4-21 h). The course of the lysis was followed angiographyically and clinically. All patients were inteerviewed by telephone 23 months (4-35 months) later.Results: In 3 patients, lysis was complete. In 5 patients, only little thrombotic material remained. In all patients, symptoms of ischemia resolved completely within the first hours after initiation of lysis. In 5 cases, bleeding occurred at the puncture site closed with the Duett device during lysis, including development of a false aneurysm in 2 cases. Complications led to premature termination (n=2) or interruption of the lysis (n=3). All complications were treated conservatively. Clinically, long-term sequelae were paresthesia and hypoesthesia in the lower leg and foot in 2 patients treated with lysis, and in the patient who underwent surgery. (orig.) [de

  5. Learning in artistic gymnastics. An experimental study with children analysing some variables in that process Aprendizaje en gimnasia artística. Un estudio experimental con niños que analiza ciertas variables del proceso

    Directory of Open Access Journals (Sweden)

    J. López

    2010-09-01

    Full Text Available

    Today the training of sports skills has lead to the conception of new approaches to attain maximum results. In practice, many teaching methods are used, yet most of the articles on motor learning or sports training refer to the total or global and the partial or analytic methods, both of interest in the field of gymnastics, offering a number of important combinations between either extreme.

    Opinions differ concerning effectiveness, and such differences also exist in gymnastics. Carrasco (1977, nevertheless, proposes "mini-circuits" as the ideal teaching method in gymnastics. In looking for a practical solution to global or analytical teaching, one experimental group study was undertaken with children participating in Sports Schools between the ages of 9 and 11. The aim was to compare the effect of three training sessions (analytical training, "mini-circuit" training, mixed training on the learning and recall of gymnastic skills.

    Interested in both final performance as well as the teaching process, the following variables were studied: motor activity time, waiting time, total number of global movements, total number of feedbacks emitted by the teacher (amount and direction, and total number of spot checks. A pre-test, post-test and re- test design was used with three groups to assess the three training sessions. Each group was trained to learn the same variable-dependent outcome.

    The results of the study showed that the "mini-circuit" training was the most effective learning and recall method. The most highly-influence process variables were both the type of aids and type of feedback provided. Overall, it is worth highlighting the importance of using the "mini-circuit" method with children. From a pedagogic perspective, this is an important finding to take into consideration, which could yield important results during schooling.
    KEY WORDS

  6. Viral lysis of photosynthesizing microbes as a mechanism for calcium carbonate nucleation in seawater

    Science.gov (United States)

    Lisle, John T.; Robbins, Lisa L.

    2016-01-01

    Removal of carbon through the precipitation and burial of calcium carbonate in marine sediments constitutes over 70% of the total carbon on Earth and is partitioned between coastal and pelagic zones. The precipitation of authigenic calcium carbonate in seawater, however, has been hotly debated because despite being in a supersaturated state, there is an absence of persistent precipitation. One of the explanations for this paradox is the geochemical conditions in seawater cannot overcome the activation energy barrier for the first step in any precipitation reaction; nucleation. Here we show that virally induced rupturing of photosynthetic cyanobacterial cells releases cytoplasmic-associated bicarbonate at concentrations ~23-fold greater than in the surrounding seawater, thereby shifting the carbonate chemistry toward the homogenous nucleation of one or more of the calcium carbonate polymorphs. Using geochemical reaction energetics, we show the saturation states (Ω) in typical seawater for calcite (Ω = 4.3), aragonite (Ω = 3.1), and vaterite (Ω = 1.2) are significantly elevated following the release and diffusion of the cytoplasmic bicarbonate (Ωcalcite = 95.7; Ωaragonite = 68.5; Ωvaterite = 25.9). These increases in Ω significantly reduce the activation energy for nuclei formation thresholds for all three polymorphs, but only vaterite nucleation is energetically favored. In the post-lysis seawater, vaterite's nuclei formation activation energy is significantly reduced from 1.85 × 10−17 J to 3.85 × 10−20 J, which increases the nuclei formation rate from highly improbable (nucleation of calcium carbonate in seawater describes a mechanism through which the initial step in the production of carbonate sediments may proceed. It also presents an additional role of photosynthesizing microbes and their viruses in marine carbon cycles and reveals these microorganisms are a collective repository for concentrated and reactive dissolved inorganic carbon (DIC

  7. Mingled Mortality: the Interplay Between Protist Grazing and Viral Lysis on Emiliania huxleyi Cell Fate

    Science.gov (United States)

    Harvey, E.; Bidle, K. D.; Johnson, M. D.

    2016-02-01

    The coccolithophore, Emiliania huxleyi plays a prominent role in global carbon cycling, as their calcite coccoliths account for a third of all oceanic calcite production. Mortality due to grazing by microzooplankton is the largest contributor to phytoplankton loss in the marine environment. However, viral infection of E. huxleyi is now thought to be as important as grazing pressure in contributing to its mortality. To understand the influence of viral infection on grazing dynamics, we examined the response of the dinoflagellate predator, Oxyrrhis marina to E. huxleyi infected with four different strains of the E. huxleyi virus (EhV). Grazing rate was significantly slower on E. huxleyi cultures that had been infected for 48 h compared to an uninfected control and this reduction in grazing rate was dependent on the strain identity of infecting EhVs. Additional experimentation indicated that grazing was the primary source of E. huxleyi loss ( 78-98%) during the first 24 h of exposure to both predator and virus. However, as viral infection progressed into the late lytic phase (48 h hour post infection), the relative contribution of grazing to total E. huxleyi mortality decreased ( 5-60%). These results suggest that mortality is partitioned along a gradient between predator-based consumption and virus-induced cell lysis, dependent on the timing of infection. Deciphering the relative importance and interactive nature of these alga-predator-viral interactions will help to elucidate the mechanisms that drive bulk measurements of phytoplankton loss, a necessary understanding to interpret and predict phytoplankton population dynamics and associated biogeochemical cycling.

  8. Chitosan as coagulant on cyanobacteria in lake restoration management may cause rapid cell lysis.

    Science.gov (United States)

    Mucci, Maíra; Noyma, Natalia Pessoa; de Magalhães, Leonardo; Miranda, Marcela; van Oosterhout, Frank; Guedes, Iamê Alves; Huszar, Vera L M; Marinho, Marcelo Manzi; Lürling, Miquel

    2017-07-01

    Combining coagulant and ballast to remove cyanobacteria from the water column is a promising restoration technique to mitigate cyanobacterial nuisance in surface waters. The organic, biodegradable polymer chitosan has been promoted as a coagulant and is viewed as non-toxic. In this study, we show that chitosan may rapidly compromise membrane integrity and kill certain cyanobacteria leading to release of cell contents in the water. A strain of Cylindrospermopsis raciborskii and one strain of Planktothrix agardhii were most sensitive. A 1.3 h exposure to a low dose of 0.5 mg l -1 chitosan already almost completely killed these cultures resulting in release of cell contents. After 24 h, reductions in PSII efficiencies of all cyanobacteria tested were observed. EC50 values varied from around 0.5 mg l -1 chitosan for the two sensitive strains, via about 5 mg l -1 chitosan for an Aphanizomenon flos-aquae strain, a toxic P. agardhii strain and two Anabaena cylindrica cultures, to more than 8 mg l -1 chitosan for a Microcystis aeruginosa strain and another A. flos-aquae strain. Differences in sensitivity to chitosan might be related to polymeric substances that surround cyanobacteria. Rapid lysis of toxic strains is likely and when chitosan flocking and sinking of cyanobacteria is considered in lake restoration, flocculation efficacy studies should be complemented with investigation on the effects of chitosan on the cyanobacteria assemblage being targeted. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  9. Phenotypic variations in osmotic lysis of Sahel goat erythrocytes in non-ionic glucose media.

    Science.gov (United States)

    Igbokwe, Nanacha Afifi; Igbokwe, Ikechukwu Onyebuchi

    2016-03-01

    Erythrocyte osmotic lysis in deionised glucose media is regulated by glucose influx, cation efflux, and changes in cell volume after water diffusion. Transmembrane fluxes may be affected by varied expression of glucose transporter protein and susceptibility of membrane proteins to glucose-induced glycosylation and oxidation in various physiologic states. Variations in haemolysis of Sahel goat erythrocytes after incubation in hyposmotic non-ionic glucose media, associated with sex, age, late pregnancy, and lactation, were investigated. The osmotic fragility curve in glucose media was sigmoidal with erythrocytes from goats in late pregnancy (PRE) or lactation (LAC) or from kid (KGT) or middle-aged (MGT) goats. Non-sigmoidal phenotype occurred in yearlings (YGT) and old (OGT) goats. The composite fragility phenotype for males and non-pregnant dry (NPD) females was non-sigmoidal. Erythrocytes with non-sigmoidal curves were more stable than those with sigmoidal curves because of inflectional shift of the curve to the left. Erythrocytes tended to be more fragile with male than female sex, KGT and MGT than YGT and OGT, and LAC and PRE than NPD. Thus, sex, age, pregnancy, and lactation affected the haemolytic pattern of goat erythrocytes in glucose media. The physiologic state of the goat affected the in vitro interaction of glucose with erythrocytes, causing variations in osmotic stability with variants of fragility phenotype. Variations in the effect of high extracellular glucose concentrations on the functions of membrane-associated glucose transporter, aquaporins, and the cation cotransporter were presumed to be relevant in regulating the physical properties of goat erythrocytes under osmotic stress.

  10. Viral lysis of photosynthesizing microbes as a mechanism for calcium carbonate nucleation in seawater

    Science.gov (United States)

    Lisle, John T.; Robbins, Lisa L.

    2016-01-01

    Removal of carbon through the precipitation and burial of calcium carbonate in marine sediments constitutes over 70% of the total carbon on Earth and is partitioned between coastal and pelagic zones. The precipitation of authigenic calcium carbonate in seawater, however, has been hotly debated because despite being in a supersaturated state, there is an absence of persistent precipitation. One of the explanations for this paradox is the geochemical conditions in seawater cannot overcome the activation energy barrier for the first step in any precipitation reaction; nucleation. Here we show that virally induced rupturing of photosynthetic cyanobacterial cells releases cytoplasmic-associated bicarbonate at concentrations ~23-fold greater than in the surrounding seawater, thereby shifting the carbonate chemistry toward the homogenous nucleation of one or more of the calcium carbonate polymorphs. Using geochemical reaction energetics, we show the saturation states (Ω) in typical seawater for calcite (Ω = 4.3), aragonite (Ω = 3.1), and vaterite (Ω = 1.2) are significantly elevated following the release and diffusion of the cytoplasmic bicarbonate (Ωcalcite = 95.7; Ωaragonite = 68.5; Ωvaterite = 25.9). These increases in Ω significantly reduce the activation energy for nuclei formation thresholds for all three polymorphs, but only vaterite nucleation is energetically favored. In the post-lysis seawater, vaterite's nuclei formation activation energy is significantly reduced from 1.85 × 10−17 J to 3.85 × 10−20 J, which increases the nuclei formation rate from highly improbable (seawater describes a mechanism through which the initial step in the production of carbonate sediments may proceed. It also presents an additional role of photosynthesizing microbes and their viruses in marine carbon cycles and reveals these microorganisms are a collective repository for concentrated and reactive dissolved inorganic carbon (DIC) that is currently not accounted for

  11. Large Gastric Perforation Sealed by Splenic Lysis: Emphasis on Indirect Signs – A Rare Case Report

    International Nuclear Information System (INIS)

    Garg, Lalit; Jain, Mansi; Taori, Kishor; Patil, Ajinky; Hatgaonkar, Anand; Rathod, Jawhar; Shah, Swenil; Patwa, Darshan; Kasat, Akshat

    2015-01-01

    Gastric perforation is a life-threatening condition, requiring early and reliable discovery. The delay before surgical treatment is a strong determinant of poor outcome, associated complications and hospitalization costs. By using ultrasound and multi-detector computed tomography (MDCT) we can further evaluate undiagnosed cases of silent gastric perforations presenting with non-specific acute abdomen. Here we bring forth the role of a radiologist in cases of perforation which present with indirect signs involving the organs forming the stomach bed, like the spleen, pancreas and kidney. A 25-year-old male patient presented with an acute onset of severe upper abdominal pain radiating to the back and vomiting. MDCT of the abdomen was done which revealed atrophic pancreas with organized collection in the sub-capsular location indenting the superior pole of the left kidney. Spleen was not visualized. The most striking imaging finding in that case was destruction of the splenic parenchyma with protrusion of the remaining tissue into the stomach lumen. The hypothesis behind this was a cascade of events which started with gastric perforation, spillage of highly destructive gastric juice over the stomach bed and finally becoming silent with rapid sealing of the defect by the omentum and the spleen. Acute abdomen is a diagnostic challenge to a clinician and radiologist with gastric perforation being a great mimicker of other urgent abdominal pathologies. To avoid a delayed diagnosis or a misdiagnosis, familiarity with typical and atypical imaging features is essential as in our case of splenic lysis. It acted as the 2 nd policeman and provided a great clue to solve the diagnostic dilemma

  12. Estudios sobre plantas andinas,- V

    Directory of Open Access Journals (Sweden)

    Cuatrecasas José

    1943-12-01

    Full Text Available Desde hace diez años, he venido interesándome por el conocimiento y clasificación del género Diplostephium, distribuido abundantemente en las zonas frías de los Andes, desde Venezuela al norte de Chile con un enclave en Costa Rica. Las excursiones realizadas en 1932 me permitieron descubrir dos nuevas especies colombianas (1 y el estudio de la colección Isern me dio oportunidad de conocer otras especies ecuatorianas y peruanas (2. A raíz del abundante material recogido en mis excursiones por Colombia desde el año 1938, decidí llevar a cabo el estudio monográfico del género; para ella diversos centros de los Estados Unidos pusieron a mi disposición sus coIecciones: Smithsonian Institution (United States National Herbarium, Field Museum of Natural History de Chicago y New York Botanical Garden, los cuales me remitieron el material para este fin al Instituto de Ciencias Naturales de Bogotá; el trabajo fue iniciado en este centro, pero al trasladarme a la Escuela Superior de Agricultura Tropical de Cali, la dirección del Instituto me permitió llevar para el Valle todos los ejemplares, incluso los del Herbario Nacional Colombiano, y así pude concluir el estudio provisional, en mi nuevo lugar de trabajo.

  13. Estudios sobre Plantas Andinas, X

    Directory of Open Access Journals (Sweden)

    Cuatrecasas José

    1967-09-01

    Full Text Available Durante la preparación del género Baccharis para Prima Flora Colombiana tuve que estudiar gran número de especies andinas, tropicales y extratropicales de fuera de Colombia; el objeto principal de tales estudios fue el de tipificar las especies íntimamente relacionadas con las colombianas y establecer su diferenciación taxonómica. La consulta de los tipos específicos y la identificación de gran número de colecciones procedentes de las regiones andinas hasta el sur del continente americano permitió precisar el concepto de bastantes especies y de su área geográfica, al mismo tiempo que el establecimiento de numerosas sinonimias.  La mayor parte de las novedades taxonómicas y de los comentarios derivados de tal estudio va incluída en el texto de las Astereae de Colombia a publicar en breve. El objeto de este artículo es dar a conocer otra parte de las novedades relativas a especies andinas de los países vecinos y describir varias entidades taxonómicas deficientemente conocidas, incorporando a su conocimiento los resultados de muchas disecciones llevadas a cabo en los mencionados estudios.  El trabajo basico para estas notas fue hecho en la Smithsonian Institution; se consultaron también las colecciones de los herbarios europeos de Londres, París, Florencia, Ginebra y Madrid en viaje hecho en otoño de 1963. National Science Foundation de Washington D.C. subvencionó los trabajos.

  14. LOS ESTUDIOS DE POBREZA URBANA

    Directory of Open Access Journals (Sweden)

    Rina De León Herrera

    2007-08-01

    Full Text Available En este artículo se intenta mostrar en forma sucinta la evolución de los estudios de pobreza urbana; se retoma para ello la producción escritural de investigadores que han hecho aportes valiosos sobre la temática en diferentes épocas y espacios geográficos. La información se ha organizado en dos unidades de análisis: la producción escritural en los países desarrollados y en los países en desarrollo.

  15. Esclavizada en los Estudios Poscoloniales

    Directory of Open Access Journals (Sweden)

    Nellys Montenegro De la Hoz

    2014-01-01

    Full Text Available Este ensayo va a centrarse en el análisis de las formas de resis - tencia e identidad esclavizada presentes en Changó, el gran putas de Manuel Zapata Olivella, a través del análisis de los discur - sos hegemónicos y subalternos que hacían parte del sistema de poder, y la intervención de las teorías o estudios poscoloniales que cuestionan dichas perspectivas para redefinir las diferencias de las identidades contradictorias y desarrollar de esta manera literaturas deslindadas de las estructuras ideológicas y hegemónicas.

  16. Concerning the role of cell lysis-cryptic growth in anaerobic side-stream reactors: the single-cell analysis of viable, dead and lysed bacteria.

    Science.gov (United States)

    Foladori, P; Velho, V F; Costa, R H R; Bruni, L; Quaranta, A; Andreottola, G

    2015-05-01

    In the Anaerobic Side-Stream Reactor (ASSR), part of the return sludge undergoes alternating aerobic and anaerobic conditions with the aim of reducing sludge production. In this paper, viability, enzymatic activity, death and lysis of bacterial cells exposed to aerobic and anaerobic conditions for 16 d were investigated at single-cell level by flow cytometry, with the objective of contributing to the understanding of the mechanisms of sludge reduction in the ASSR systems. Results indicated that total and viable bacteria did not decrease during the anaerobic phase, indicating that anaerobiosis at ambient temperature does not produce a significant cell lysis. Bacteria decay and lysis occurred principally under aerobic conditions. The aerobic decay rate of total bacteria (bTB) was considered as the rate of generation of lysed bacteria. Values of bTB of 0.07-0.11 d(-1) were measured in anaerobic + aerobic sequence. The enzymatic activity was not particularly affected by the transition from anaerobiosis to aerobiosis. Large solubilisation of COD and NH4(+) was observed only under anaerobic conditions, as a consequence of hydrolysis of organic matter, but not due to cell lysis. The observations supported the proposal of two independent mechanisms contributing equally to sludge reduction: (1) under anaerobic conditions: sludge hydrolysis of non-bacterial material, (2) under aerobic conditions: bacterial cell lysis and oxidation of released biodegradable compounds. Copyright © 2015 Elsevier Ltd. All rights reserved.

  17. A Simple Method for DNA Extraction from Mature Date Palm Leaves: Impact of Sand Grinding and Composition of Lysis Buffer

    Science.gov (United States)

    Arif, Ibrahim A.; Bakir, Mohammad A.; Khan, Haseeb A.; Ahamed, Anis; Al Farhan, Ahmad H.; Al Homaidan, Ali A.; Al Sadoon, Mohammad; Bahkali, Ali H.; Shobrak, Mohammad

    2010-01-01

    Molecular marker techniques have been widely used for cultivar identification of inbred date palms (Phoenix dactylifera L.; Arecaceae) and biodiversity conservation. Isolation of highly pure DNA is the prerequisite for PCR amplification and subsequent use such as DNA fingerprinting and sequencing of genes that have recently been developed for barcoding. To avoid problems related to the preservation and use of liquid nitrogen, we examined sterile sand for grinding the date palm leaves. Individual and combined effects of sodium chloride (NaCl), polyvinylpyrrolidone (PVP) and lithium chloride (LiCl) with the cetyltrimethylammonium bromide (CTAB) method for a DNA yield of sufficient purity and PCR amplification were evaluated in this study. Presence of LiCl and PVP alone or together in the lysis buffer did not significantly improve the DNA yield and purity compared with the addition of NaCl. Our study suggested that grinding of date palm leaf with sterile sand and inclusion of NaCl (1.4 M) in the lysis buffer without the costly use of liquid nitrogen, PVP and LiCl, provides a DNA yield of sufficient purity, suitable for PCR amplification. PMID:20957085

  18. Studies on cytotoxic and clot lysis activity of probiotically fermented cocktail juice prepared using Camellia sinensis and Punica grantum

    Science.gov (United States)

    Biswas, Ananya; Deori, Meenakshi; Nivetha, A.; Mohansrinivasan, V.

    2017-11-01

    In the current research the effect of probiotic microorganisms viz; Lactococcus lactis and Lactobacillus plantarum on fermentation of Camellia sinensis and Punica grantum was studied. In vitro test were done to analyze the anticancer, antioxidant and atherosclerosis (clot lysis) properties of fermented juice. The juice was fermented for 48 and 96h, during which concentration of phenolic content, total acid content and free radical scavenging activity of the sample was analyzed by DPPH assay (α, α-diphenyl-β-picrylhydrazyl). Dropping of pH was observed after 48 h of fermentation. The clot lysis activity was found to be 80 % in 100μl concentration of fermented cocktail juice. The 96 h fermented sample has shown around 70% inhibition against colon cancer cell lines. Analytical study of HPLC proves the organic acid production such as ascorbic acid in superior amount for 96h of fermented sample, Based on the retention time, the corresponding peaks were detected at 4.919 and 4.831 min.

  19. Enhanced lysis of herpes simplex virus type 1-infected mouse cell lines by NC and NK effectors

    Energy Technology Data Exchange (ETDEWEB)

    Colmenares, C.; Lopez, C.

    1986-05-01

    Spontaneously cytotoxic murine lymphocytes lysed certain cell types infected by herpes simplex virus type 1 (HSV-1) better than uninfected cells. Although HSV-1 adsorbed to the surface of all the target cells, those in which the virus replicated more efficiently were lysed to a greater extent. As targets, the authors used cell lines that, when uninfected, were spontaneously lysed by NK cells (YAC-1) or by NC cells (WEHI-164). They also used a fibroblastoid cell line (M50) and a monocytic tumor line (PU51R), which were not spontaneously killed. NK cells lysed HSV-1-infected YAC cells better than uninfected cells, and an NC-like activity selectively lysed HSV-1-infected WEHI cells. These findings were consistent with the results of experiments performed to define the role of interferon in induction of virus-augmented cytolysis. Increased lysis of YAC-HSV and PU51R-HSV was entirely due to interferon activation and was completely abolished by performing the /sup 51/Cr-release assay in the presence of anti-interferon serum. The data show that HSV-1 infection of NK/NC targets induces increased cytotoxity, but the effector cell responsible for lysis is determined by the uninfected target, or by an interaction between the virus and target cell, rather than by a viral determinant alone.

  20. Organic and Inorganic Nitrogen Impact Chlorella variabilis Productivity and Host Quality for Viral Production and Cell Lysis.

    Science.gov (United States)

    Cheng, Yu-Shen; Labavitch, John; VanderGheynst, Jean S

    2015-05-01

    Microalgae have been proposed as a potential feedstock for biofuel production; however, cell disruption is usually required for collection and utilization of cytoplasmic polysaccharides and lipids. Virus infection might be one approach to disrupt the cell wall. The concentration of yeast extract and presence of KNO3 in algae cultivation media were investigated to observe their effects on Chlorella variabilis NC64A physiology and composition and the subsequent effect on production of Chlorella virus and disruption of infected cells. Cytoplasmic starch accumulation increased from 5% to approximately 35% of the total dry weight when yeast extract decreased from 1 to 0.25 g L(-1). When cells were cultured with the lowest nitrogen levels, the total polysaccharide accounted for more than 50% of the cell wall, which was 1.7 times higher than the content in cells cultured with the highest nitrogen levels. The C/N ratio of the algal biomass decreased by a factor of approximately 2 when yeast extract increased from 0.25 to 1 g L(-1). After virus infection, cells with a low C/N ratio produced a 7.6 times higher burst size than cells with a high C/N ratio, suggesting that the nitrogen content in C. variabilis has a large influence on viral production and cell lysis. The results have implications on management of nitrogen for both the synthesis of products from algae and product recovery via viral lysis.

  1. Urate oxidase for the prevention and treatment of tumour lysis syndrome in children with cancer.

    Science.gov (United States)

    Cheuk, Daniel Kl; Chiang, Alan Ks; Chan, Godfrey Cf; Ha, Shau Yin

    2017-03-08

    Tumour lysis syndrome (TLS) is a serious complication of malignancies and can result in renal failure or death. Previous reviews did not find clear evidence of benefit of urate oxidase in children with cancer. This review is the second update of a previously published Cochrane review. To assess the effects and safety of urate oxidase for the prevention and treatment of TLS in children with malignancies. In March 2016 we searched CENTRAL, MEDLINE, Embase, and CINAHL. In addition, we searched the reference lists of all identified relevant papers, trials registers and other databases. We also screened conference proceedings and we contacted experts in the field and the manufacturer of rasburicase, Sanofi-aventis. Randomised controlled trials (RCT) and controlled clinical trials (CCT) of urate oxidase for the prevention or treatment of TLS in children under 18 years with any malignancy. Two review authors independently extracted trial data and assessed individual trial quality. We used risk ratios (RR) for dichotomous data and mean difference (MD) for continuous data. We included seven trials, involving 471 participants in the treatment groups and 603 participants in the control groups. No new studies were identified in the update. One RCT and five CCTs compared urate oxidase and allopurinol. Three trials tested Uricozyme, and three trials tested rasburicase for the prevention of TLS.The RCT did not evaluate the primary outcome (incidence of clinical TLS). It showed no clear evidence of a difference in mortality (both all-cause mortality (Fisher's exact test P = 0.23) and mortality due to TLS (no deaths in either group)), renal failure (Fisher's exact test P = 0.46), and adverse effects between the treatment and the control groups (Fisher's exact test P = 1.0). The frequency of normalisation of uric acid at four hours (10 out of 10 participants in the treatment group versus zero out of nine participants in the control group, Fisher's exact test P oxidase (RR 9.10, 95

  2. Application of Doehlert experimental design in the optimization of experimental variables for the Pseudozyma sp. (CCMB 306 and Pseudozyma sp. (CCMB 300 cell lysis

    Directory of Open Access Journals (Sweden)

    Amanda Reges de Sena

    2012-12-01

    Full Text Available This study aimed to verify the influence of pH and temperature on the lysis of yeast using experimental design. In this study, the enzymatic extract containing β-1,3-glucanase and chitinase, obtained from the micro-organism Moniliophthora perniciosa, was used. The experiment showed that the best conditions for lysis of Pseudozyma sp. (CCMB 306 and Pseudozyma sp. (CCMB 300 by lytic enzyme were pH 4.9 at 37 ºC and pH 3.9 at 26.7 ºC, respectively. The lytic enzyme may be used for obtaining various biotechnology products from yeast.

  3. Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices

    Directory of Open Access Journals (Sweden)

    Philippe Renaud

    2012-08-01

    Full Text Available Here we present an electrical lysis throughput of 600 microliters per minute at high cell density (108 yeast cells per ml with 90% efficiency, thus improving the current common throughput of one microliter per minute. We also demonstrate the extraction of intracellular luciferase from mammalian cells with efficiency comparable to off-chip bulk chemical lysis. The goal of this work is to develop a sample preparation module that can act as a stand-alone device or be integrated to other functions already demonstrated in miniaturized devices, including sorting and analysis, towards a true lab-on-a-chip.

  4. RASCAL [Radiological Assessment System for Consequence AnaLysis]: A screening model for estimating doses from radiological accidents

    International Nuclear Information System (INIS)

    Sjoreen, A.L.; Athey, G.F.; Sakenas, C.A.; McKenna, T.J.

    1988-01-01

    The Radiological Assessment System for Consequence AnaLysis (RASCAL) is a new MS-DOS-based dose assessment model which has been written for the US Nuclear Regulatory Commission for use during response to radiological emergencies. RASCAL is designed to provide crude estimates of the effects of an accident while the accident is in progress and only limited information is available. It has been designed to be very simple to use and to run quickly. RASCAL is unique in that it estimates the source term based on fundamental plant conditions and does not rely solely on release rate estimation (e.g., Ci/sec of I-131). Therefore, it can estimate consequences of accidents involving unmonitored pathways or projected failures. RASCAL will replace the older model, IRDAM. 6 refs

  5. Detection of fungal DNA in lysis-centrifugation blood culture for the diagnosis of invasive candidiasis in neonatal patients.

    Science.gov (United States)

    Trovato, L; Betta, P; Romeo, M G; Oliveri, S

    2012-03-01

    We report data concerning the detection of fungal DNA directly from lysis-centrifugation blood culture to assess its value in the detection of fungaemia in 86 of the 347 patients admitted to the neonatal intensive-care unit between January 2009 and December 2010. The sensitivity and specificity of the PCR were 87.5% and 98.5%, respectively, with a positive predictive value of 93.3% and a negative predictive value of 97.1%. Detection of fungal DNA directly from blood culture Isolator 1.5 microbial tubes, without prior cultivation, is a promising approach for the rapid detection of Candida spp. in neonates with suspected candidaemia. © 2011 The Authors. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases.

  6. Influence of environmental variation on the bacterioplankton community and its loss to viral lysis in the Curonian Lagoon

    Science.gov (United States)

    Šulčius, Sigitas; Reunamo, Anna; Paškauskas, Ričardas; Leskinen, Piia

    2018-05-01

    Coastal lagoons are continuously exposed to strong environmental gradients that determine the distribution and trophic interactions of microbial communities. Therefore, in this study we assessed whether and how environmental changes influence the bacterial community and its vulnerability to viral infection and lysis along the major environmental gradient in the Curonian Lagoon. We found significant differences in bacterial community profiles, their richness and evenness between the riverine, freshwater southern part and the Baltic Sea water intrusion-influenced northern part of the lagoon, suggesting strong environmental control of the structure of bacterial communities. Viruses were found to be play an important role in bacterial mortality in the Curonian Lagoon, being responsible for the removal of 20-50% of the bacterial standing stock. We observed differences in virioplankton decay rates and virus burst sizes between the northern and southern parts of the lagoon. However, no relationships were found between viral activity and bacterial communities within the lagoon ecosystem. The frequency of infected cells and virus-mediated bacterial mortality (VMBM) remained constant among the sampling sites irrespective of differences in bacteria community assemblages and environmental conditions. The results indicate that factors determining changes in bacterial diversity are different from the factors limiting their vulnerability to viral infection and lysis. This study also suggests that under changing environmental conditions, virus-bacteria interactions are more stable than the interacting viral and bacterial communities themselves. These findings are important for understanding the functioning of the coastal ecosystems under the rapidly changing local (spatial and temporal) and global (e.g. eutrophication, climate change) conditions.

  7. Regulated programmed lysis of recombinant Salmonella in host tissues to release protective antigens and confer biological containment.

    Science.gov (United States)

    Kong, Wei; Wanda, Soo-Young; Zhang, Xin; Bollen, Wendy; Tinge, Steven A; Roland, Kenneth L; Curtiss, Roy

    2008-07-08

    We have devised and constructed a biological containment system designed to cause programmed bacterial cell lysis with no survivors. We have validated this system, using Salmonella enterica serovar Typhimurium vaccines for antigen delivery after colonization of host lymphoid tissues. The system is composed of two parts. The first component is Salmonella typhimurium strain chi8937, with deletions of asdA and arabinose-regulated expression of murA, two genes required for peptidoglycan synthesis and additional mutations to enhance complete lysis and antigen delivery. The second component is plasmid pYA3681, which encodes arabinose-regulated murA and asdA expression and C2-regulated synthesis of antisense asdA and murA mRNA transcribed from the P22 P(R) promoter. An arabinose-regulated c2 gene is present in the chromosome. chi8937(pYA3681) exhibits arabinose-dependent growth. Upon invasion of host tissues, an arabinose-free environment, transcription of asdA, murA, and c2 ceases, and concentrations of their gene products decrease because of cell division. The drop in C2 concentration results in activation of P(R), driving synthesis of antisense mRNA to block translation of any residual asdA and murA mRNA. A highly antigenic alpha-helical domain of Streptococcus pneumoniae Rx1 PspA was cloned into pYA3681, resulting in pYA3685 to test antigen delivery. Mice orally immunized with chi8937(pYA3685) developed antibody responses to PspA and Salmonella outer membrane proteins. No viable vaccine strain cells were detected in host tissues after 21 days. This system has potential applications with other Gram-negative bacteria in which biological containment would be desirable.

  8. Perdurabilidad empresarial: concepto, estudios, hallazgos

    Directory of Open Access Journals (Sweden)

    Hugo Alberto Rivera Rodríguez

    2012-06-01

    Full Text Available La literatura en dirección de empresas durante los últimos 20 años ha analizado diferentes aspectos de la perdurabilidad empresarial, sobre todo lo relacionado con el papel del líder, la asociación con la rentabilidad, y los efectos que genera en el tejido empresarial. Sin embargo, aún existen muchos aspectos en los cuales se debe profundizar. El primero de ellos es dar solución a la diversidad terminológica existente. Al no tener un consenso sobre el concepto, se utilizan de manera indiscriminada términos como longevidad, sostenibilidad, éxito, supervivencia, duración, etc., los cuales en lugar de aclarar, generan confusión. Por otro lado, tampoco hay evidencia suficiente para sugerir alternativas que permitan a las empresas lograr ser perdurables. El artículo que se presenta se fundamenta en una revisión exhaustiva del concepto de perdurabilidad empresarial, y hace una relación de los principales hallazgos encontrados en la literatura sobre las condiciones que pueden facilitar la perdurabilidad empresarial. Con ello se pretende de avanzar en el estudio de la relación entre la perdurabilidad, el comportamiento estratégico de las empresas y desempeño financiero.

  9. Using SPIRAL (Single Pollen Isotope Ratio AnaLysis) to estimate C 3- and C 4-grass abundance in the paleorecord

    Science.gov (United States)

    Nelson, David M.; Hu, Feng Sheng; Scholes, Daniel R.; Joshi, Neeraj; Pearson, Ann

    2008-05-01

    C 3 and C 4 grasses differ greatly in their responses to environmental controls and influences on biogeochemical processes (e.g. water, carbon, and nutrient cycling). Difficulties in distinguishing between these two functional groups of grasses have hindered paleoecological studies of grass-dominated ecosystems. Stable carbon isotopic analysis of individual grains of grass pollen using a spooling-wire microcombustion device interfaced with an isotope-ratio mass spectrometer holds promise for improving C 3 and C 4 grass reconstructions. This technique, SPIRAL (Single Pollen Isotope Ratio AnaLysis), has only been evaluated using pollen of known C 3 and C 4 grasses. To test the ability of SPIRAL to reproduce the abundance of C 3 and C 4 grasses on the landscape, we measured δ13C values of > 1500 individual grains of grass pollen isolated from the surface sediments of ten lakes in areas that span a large gradient of C 3- and C 4-grass abundance, as determined from vegetation surveys. Results indicate a strong positive correlation between the δ13C-based estimates of % C 4-grass pollen and the abundance of C 4 grasses on the landscape. The % C 4-grass pollen slightly underestimates the actual abundance of C 4 grasses at sites with high proportions of C 4 grasses, which can be corrected using regression analysis. Comparison of the % C 4-grass pollen with C/N and δ13C measurements of bulk organic matter illustrates the distinct advantages of grass-pollen δ13C as a proxy for distinguishing C 3 and C 4 shifts within the grass family. Thus SPIRAL promises to advance our understanding of grassland ecology and evolution.

  10. Funciones profesorales: un estudio factorial

    Directory of Open Access Journals (Sweden)

    Pedro S. de VICENTE RODRÍGUEZ

    2009-11-01

    Full Text Available La preocupación por la «cantidad» ha privado durante mucho tiempo en el ámbito educativo; ha sido considerado esencial por los gobiernos la consecución de una «educación para todos». Sin embargo, el aumento de los costos no ha conllevado una mejora sustancial, una disminución efectiva de la ineficacia de los sistemas tradicionales de enseñanza; de ello dan fe las tasas de abandonos y repeticiones de curso. Esto conduce a considerar que la falta de correspondencia entre el aumento de los recursos destinados a educación y el rendimiento de las instituciones educativas sea un problema de «calidad» más que de «cantidad». De ahí que se hable hoy insistentemente de la mejora de la calidad de la educación. Al tema de la «calidad de la educación» se dedicó en 1981 un seminario organizado por la Escuela Asturiana de estudios Hispánicos. En él, Víctor García Hoz definía la calidad educativa «como el modo de ser de la educación que reúne las características de integridad, coherencia y eficacia»1, entendiendo la eficacia como la denotación de que el fin perseguido ha sido alcanzado, más que como producción de algo material, con lo que es posible hablar en términos de eficacia refiriéndose tanto a lo cuantitativo como a lo cualitativo.

  11. Estudio comparativo de la productividad y calidad obtenidas en la soldadura de tubos de calidad T9 empleados en el sector petroquímico, mediante los procesos TIG, HW-TIG y PAW Comparative study of productivity and quality obtained in tube welding quality T9 employees in the petrochemical industry, through TIG, HW-TIG and PAW processes

    Directory of Open Access Journals (Sweden)

    César García González

    2012-09-01

    Full Text Available Tradicionalmente, la fabricación soldada de tuberías de acero Cr-Mo empleadas en serpentines de hornos para la industria petroquímica se ha venido realizando de forma masiva mediante procesos de baja productividad, principalmente TIG para la pasada de raíz y SMAW para las pasadas de relleno, ambos manuales. En este estudio se muestran los resultados de la investigación realizada por el Centro Tecnológico AIMEN, en relación al empleo de tecnologías de alto rendimiento, concretamente TIG, HW-TIG y PAW robotizadas, aplicadas a los mismos materiales y productos. De este modo se calculan y comparan cuatro índices de productividad: Tiempo de Soldadura, Tasa de Deposición, Material Depositado y Volumen de Gas Consumido. El objetivo del estudio es contribuir a la reducción de los tiempos de producción, de los costes de fabricación, y a la mejora de la calidad del producto.Traditionally, the manufacture of welded Cr-Mo steel pipes used in furnace coils for petrochemical industry has been carried out on a massive scale by low productivity processes, mainly TIG for root pass and SMAW for layers of filling run, both of them manuals. This study presents the results of a research, conducted by AIMEN Technology Centre, related to the use of high performance technologies, specifically robotic processes such as TIG, TIG-HW and PAW, applied to the same materials and products. Thus, four productivity ratios have been calculated and compared: welding time, deposition rate, deposited material and volume of consumed gas. The aim of the study is to contribute to the reduction of the production time, manufacturing costs and to the improvement of the product quality.

  12. Flow Rate Capacity Reduction Due to Temporal and Dynamic Processes in Large Pipelines. Study with Field Measurements; Efectos dinamicos y temporales en la reduccion de la capacidad de conduccion en grandes acueductos. Estudio con medidas en prototipo

    Energy Technology Data Exchange (ETDEWEB)

    Carmona Paredes, Rafael; Ortiz Nunez, Luis Alfonso; Sanchez Huerta, Alejandro [Universidad Nacional Autonoma de Mexico (Mexico)

    2002-06-01

    More than 15 years of operation have show that some water transport pressurized pipelines change their flow rate capacity faster than expected due to normal roughness increase. As explained by the tubular pinch effects, the radial migration of suspended particles in a flow can produce a high concentration close to the pipe wall. The non-uniform particle concentration leads to higher velocities at the center of the tube, equivalent to a reduced hydraulic section that increases the head losses. A model to explain field measurements at the Chapala-Guadalajara Aqueduct is proposed that suggests to hydraulic engineers to be more distrustful when using traditional head loss formulas to analyze water transport pipelines. [Spanish] La perdida de la capacidad de conduccion es un grave problema en la operacion de grandes acueductos. Mas de 15 anos de estudios y de inspeccion directa al interior de las tuberias de varios sistemas de abastecimiento de agua potable han mostrado el desarrollo de capas de material fino fuertemente adheridas a la pared de los tubos. En algunos casos, la variacion de la perdida de carga no ha podido ser explicada con los modelos tradicionales de crecimiento de la rugosidad interna, por lo que para explicar de forma adecuada las mediciones de campo realizadas en el acueducto Chapala-Guadalajara ha sido necesario incorporar de manera simultanea fenomenos dinamicos y temporales. Con base en el fecto de elongacion tubular (o tubular pinch effect), descrito por otros autores, mas observaciones directas al interior de las tuberias, en este trabajo se plantea como una posible de la disminucion de capacidad de conduccion en el acueducto Chapala-Guadalajara un cambio aparente en la seccion efectiva del flujo, originado por la migracion radial hacia la pared del tubo de las particulas suspendidas en el agua. El modelo que propone reproduce las medidas en campo con diferencias menores al 10% e invita a reflexionar sobre las practicas convencionales para

  13. Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1

    NARCIS (Netherlands)

    Fernández Ramírez, Mónica D.; Nierop Groot, Masja N.; Smid, Eddy J.; Hols, Pascal; Kleerebezem, Michiel; Abee, Tjakko

    2018-01-01

    Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products. This study provides new insights into biofilm

  14. Estudio de similitud del proceso de gestión de riesgos en proyectos de outsourcing de software: utilización de un método Similarity study of risk management process in software outsourcing projects: using a method

    Directory of Open Access Journals (Sweden)

    Gloria Piedad Gasca Hurtado

    2010-07-01

    Full Text Available En este artículo, se realiza un estudio de estándares y modelos de ingeniería de software con el fin de determinar similitudes entre los mismos, desde el punto de vista del proceso de gestión de riesgos en el ámbito de outsourcing de software. Para seleccionar y analizar dichos estándares y modelos de manera formal, se utiliza el método MSSS desarrollado a partir de un profundo análisis de diferentes mapeos de estándares y modelos, que dan lugar una serie de pasos o pautas que permiten formalizar y organizar el estudio. Esta investigación permite, además de determinar las similitudes, hacer una comparación de cada estándar y modelo, previa selección y clasificación de los mismos según el ámbito en el que se enfoca.In this paper, a study of software engineering standards and models to determine similarities between them has been carried out, from the viewpoint of risk management process in the field of software outsourcing. To select and review these standards and models, MSSS model was used, which was developed from a thorough analysis of different mapping standards and models, leading a series of steps or guidelines to help formalize and organize the study. Besides determining similarities, this research makes a comparison of each standard and model, prior selection and classification of them, according to the area it focuses.

  15. Thermodynamic study of residual heat from a high temperature nuclear reactor to analyze its viability in cogeneration processes; Estudio termodinamico del calor residual de un reactor nuclear de alta temperatura para analizar su viabilidad en procesos de cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Santillan R, A.; Valle H, J.; Escalante, J. A., E-mail: santillanaura@gmail.com [Universidad Politecnica Metropolitana de Hidalgo, Boulevard acceso a Tolcayuca 1009, Ex-Hacienda San Javier, 43860 Tolcayuca, Hidalgo (Mexico)

    2015-09-15

    In this paper the thermodynamic study of a nuclear power plant of high temperature at gas turbine (GTHTR300) is presented for estimating the exploitable waste heat in a process of desalination of seawater. One of the most studied and viable sustainable energy for the production of electricity, without the emission of greenhouse gases, is the nuclear energy. The fourth generation nuclear power plants have greater advantages than those currently installed plants; these advantages have to do with security, increased efficiencies and feasibility to be coupled to electrical cogeneration processes. In this paper the thermodynamic study of a nuclear power plant type GTHTR300 is realized, which is selected by greater efficiencies and have optimal conditions for use in electrical cogeneration processes due to high operating temperatures, which are between 700 and 950 degrees Celsius. The aim of the study is to determine the heat losses and the work done at each stage of the system, determining where they are the greatest losses and analyzing in that processes can be taken advantage. Based on the study was appointed that most of the energy losses are in form of heat in the coolers and usually this is emitted into the atmosphere without being used. From the results a process of desalination of seawater as electrical cogeneration process is proposed. This paper contains a brief description of the operation of the nuclear power plant, focusing on operation conditions and thermodynamic characteristics for the implementation of electrical cogeneration process, a thermodynamic analysis based on mass and energy balance was developed. The results allow quantifying the losses of thermal energy and determining the optimal section for coupling of the reactor with the desalination process, seeking to have a great overall efficiency. (Author)

  16. Study of the pyrochemical treatment-recycling process of the Molten Salt Reactor fuel; Estudio de sistema de un proceso de tratamiento-reciclaje piroquimico del combustible de un reactor de sales fundidas

    Energy Technology Data Exchange (ETDEWEB)

    Boussier, H.; Heuer, D.

    2010-07-01

    The Separation Processes Studies Laboratory (Commissariat a l'energie Atomique) has made a preliminary assessment of the reprocessing system associated with Molten Salt Fast Reactor (MSFR). The scheme studied in this paper is based on the principle of reductive extraction and metal transfer that constituted the core process designed for the Molten Salt Breeder Reactor (MSBR), although the flow diagram has been adapted to the current needs of the Molten Salt Fast Reactor (MSFR).

  17. Preliminary degradation process study of infectious biological waste in a 5 k W thermal plasma equipment.; Estudio Preliminar de Proceso de degradacion de residuos biologico-infecciosos en un equipo de plasma termico de 5 kW

    Energy Technology Data Exchange (ETDEWEB)

    Xochihua S M, M C

    1998-12-31

    This work is a preliminary study of infectious biological waste degradation process by thermal plasma and was made in Thermal Plasma Applications Laboratory of Environmental Studies Department of the National Institute of Nuclear Research (ININ). Infectious biological waste degradation process is realized by using samples such polyethylene, cotton, glass, etc., but the present study scope is to analyze polyethylene degradation process with mass and energy balances involved. Degradation method is realized as follow: a polyethylene sample is put in an appropriated crucible localized inside a pyrolysis reactor chamber, the plasma jet is projected to the sample, by the pyrolysis phenomena the sample is degraded into its constitutive particles: carbon and hydrogen. Air was utilized as a recombination gas in order to obtain the higher percent of CO{sub 2} if amount of O{sub 2} is greater in the recombination gas, the CO generation is reduced. The effluent gases of exhaust pyrolysis reactor through are passed through a heat exchanger to get cooled gases, the temperature water used is 15 Centigrade degrees. Finally the gases was tried into absorption tower with water as an absorbent fluid. Thermal plasma degradation process is a very promising technology, but is necessary to develop engineering process area to avail all advantages of thermal plasma. (Author).

  18. Olive oil glycero lysis with the immobilized lipase Candida antarctica in a solvent free system

    Energy Technology Data Exchange (ETDEWEB)

    Singh, A. K.; Mukhopadhyay, M.

    2012-11-01

    In the present work, the solvent free lipase glycerolysis of olive oil for the production of monoglyceride (MG) and diglyceride (DG) with an immobilized Lipase B Candida antarctica was studied. The experiments were performed in batch mode by varying different process parameters. The Results showed that the MG and DG yields were dependent on operating conditions such as time, temperature, glycerol/ oil molar ratio, enzyme concentration and the water content in glycerol. The optimum operating time for maximum MG, 26 wt% and DG, 30 wt% production was 3h. The initial reaction rate was studied by varying different process parameters for 1h. The initial reaction rate increased at 30 degree centigrade temperature, 2:1 glycerol/oil molar ratio, 3.5% (w/w) water content in glycerol and 0.015g of enzyme loading. Comparative data for MG and DG yields for different oils and enzyme combinations were presented.

  19. Sub-apoptotic dosages of pro-oxidant vitamin cocktails sensitize human melanoma cells to NK cell lysis.

    Science.gov (United States)

    Tremante, Elisa; Santarelli, Lory; Lo Monaco, Elisa; Sampaoli, Camilla; Ingegnere, Tiziano; Guerrieri, Roberto; Tomasetti, Marco; Giacomini, Patrizio

    2015-10-13

    Alpha-tocopheryl succinate (αTOS), vitamin K3 (VK3) and vitamin C (ascorbic acid, AA) were previously shown to synergistically promote different death pathways in carcinoma cells, depending on their concentrations and combinations. Similar effects were observed herein in melanoma cells, although αTOS behaved as an antagonist. Interestingly, suboptimal cell death-inducing concentrations (1.5 μM αTOS/20 μM AA/0.2 μM VK3) effectively up-regulated activating Natural Killer (NK) cell ligands, including MICA (the stress-signaling ligand of the NKG2D receptor), and/or the ligands of at least one of the natural cytotoxicity receptors (NKp30, NKp44 and NKp46) in 5/6 melanoma cell lines. Only an isolated MICA down-regulation was seen. HLA class I, HLA class II, ULBP1, ULBP2, ULBP3, Nectin-2, and PVR displayed little, if any, change in expression. Ligand up-regulation resulted in improved lysis by polyclonal NK cells armed with the corresponding activating receptors. These results provide the first evidence for concerted induction of cell death by cell-autonomous and extrinsic (immune) mechanisms. Alarming the immune system much below the cell damage threshold may have evolved as a sensitive readout of neoplastic transformation and oxidative stress. Cocktails of vitamin analogues at slightly supra-physiological dosages may find application as mild complements of melanoma treatment, and in chemoprevention.

  20. Early postnatal diagnosis of hereditary spherocytosis by combining light microscopy, acidified glycerol lysis test and eosin-5'-maleimide binding assay.

    Science.gov (United States)

    Andres, Oliver; Eber, Stefan; Speer, Christian P

    2015-12-01

    Exact diagnosis of hereditary spherocytosis (HS) is widely considered unreliable around birth. However, early postnatal diagnosis at the beginning of congenital hemolysis may be essential for managing neonatal anemia and hemolytic icterus, identifying those at high risk for severe hyperbilirubinemia, irreversible kernicterus, or sudden need for red cell transfusion. We analyzed 37 blood samples from neonates or infants up to six weeks of life that had been collected in-house or shipped to our laboratory due to suspected red cell membrane disorder. By combining assessment of red cell morphology, acidified glycerol lysis test (AGLT), and eosin-5'-maleimide (EMA) binding assay, we were able to clearly exclude HS in 22 and confirm HS in 10 patients, of which one had undergone red cell transfusion prior to blood sampling. Assessment of red cell morphology and normal test results allowed diagnosis of infantile pyknocytosis or Heinz body anemia in three neonates. Re-evaluation of five patients with inconsistent results of AGLT and EMA binding led to confirmation of HS in two cases. Automated analysis of hematologic parameters revealed elevated proportion of hyperdense cells to be a highly significant indicator for HS in neonatal infants. We showed that assessment of red cell morphology in combination with AGLT and EMA binding assay is a reliable basis for confirming or rejecting suspected diagnosis of HS even in neonates. Our data underline the necessity for blood sampling and laboratory exploration in suspected red cell membrane or enzyme defects at the earliest occasion.

  1. High-intensity focused ultrasound sonothrombolysis: the use of perfluorocarbon droplets to achieve clot lysis at reduced acoustic power.

    Science.gov (United States)

    Pajek, Daniel; Burgess, Alison; Huang, Yuexi; Hynynen, Kullervo

    2014-09-01

    The purpose of this study was to evaluate use of intravascular perfluorocarbon droplets to reduce the sonication power required to achieve clot lysis with high-intensity focused ultrasound. High-intensity focused ultrasound with droplets was initially applied to blood clots in an in vitro flow apparatus, and inertial cavitation thresholds were determined. An embolic model for ischemic stroke was used to illustrate the feasibility of this technique in vivo. Recanalization with intravascular droplets was achieved in vivo at 24 ± 5% of the sonication power without droplets. Recanalization occurred in 71% of rabbits that received 1-ms pulsed sonications during continuous intravascular droplet infusion (p = 0.041 vs controls). Preliminary experiments indicated that damage was confined to the ultrasonic focus, suggesting that tolerable treatments would be possible with a more tightly focused hemispheric array that allows the whole focus to be placed inside of the main arteries in the human brain. Copyright © 2014 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

  2. Thrombin activatable fibrinolysis inhibitor and clot lysis time in pregnant patients with antiphospholipid syndrome: relationship with pregnancy outcome and thrombosis.

    Science.gov (United States)

    Martinez-Zamora, Maria Angeles; Tassies, Dolors; Carmona, Francisco; Espinosa, Gerard; Cervera, Ricard; Reverter, Juan Carlos; Balasch, Juan

    2009-12-01

    Antiphospholipid syndrome (APS) pregnancies are associated with thrombotic obstetric complications, despite treatment. This study evaluated Thrombin Activatable Fibrinolysis Inhibitor (TAFI) levels, TAFI gene polymorphisms and Clot Lysis Time (CLT) in pregnant patients with APS in relation to pregnancy outcome and thrombosis. Group 1 consisted of 67 pregnant patients with APS. Group 2 included 66 pregnant patients with uneventful term pregnancies and delivery. Patients were sampled during each trimester and at baseline. TAFI antigen and CLT and two polymorphisms of the TAFI gene, Ala147Thr and +1542C/G, were determined. Significantly prolonged CLT was found at baseline in Group 1. Allele distribution of the TAFI gene polymorphisms was similar in both groups. Basal TAFI and CLT in patients with APS having an adverse or a good obstetrical outcome were similar. Comparison of TAFI and CLT baseline levels in patients with APS with or without previous thrombosis showed no statistical differences. Patients with APS have impairment in fibrinolysis evidenced by prolonged CLT at baseline. TAFI and CLT do not seem to be useful as markers of obstetric outcome or risk of thrombosis in patients with APS.

  3. Genome scan of clot lysis time and its association with thrombosis in a protein C deficient kindred

    Science.gov (United States)

    Meltzer, M.E.; Hasstedt, S.J.; Vossen, C.Y.; Callas, P.W.; de Groot, Ph.G.; Rosendaal, F.R.; Lisman, T.; Bovill, E.G.

    2011-01-01

    Summary Background Previously we found increased clot lysis time (CLT), as measured with a plasma-based assay, to increase the risk of venous thrombosis in two population-based case-control studies. Genes influencing CLT are yet unknown. Objectives and Patients/Methods We tested CLT as risk factor for venous thrombosis in Kindred Vermont II (n=346), a pedigree suffering from a high thrombosis risk, partially attributable to a type I protein C deficiency. Furthermore we tested for quantitative trait loci (QTL) for CLT using variance component linkage analysis. Results Protein C deficient family members had shorter CLT than non-deficient members (median CLT 67 versus 75 minutes). One standard deviation increase in CLT increased risk of venous thrombosis 2.4-fold in non-deficient family members. Protein C deficiency without elevated CLT increased risk 6.9-fold. Combining both risk factors yielded a 27.8-fold increased risk. Heritability of CLT was 42-52%. We found suggestive evidence of linkage on chromosome 11 (62 cM), partly explained by the prothrombin 20210A mutation, and on chromosome 13 (52 cM). Thrombin Activatable Fibrinolysis Inhibitor genotypes did not explain the variation in CLT. Conclusion Hypofibrinolysis appears to increase thrombosis risk in this family especially in combination with protein C deficiency. Protein C deficiency is associated with short CLT. CLT is partly genetically regulated. Suggestive QTL were found on chromosome 11 and 13. PMID:21575129

  4. Research on the Primary Processes of Radiation-Initiated Polymerization; Recherches sur les processus primaires de polymerisation amorcee par rayonnements; Issledovaniya osnovnykh protsessov polimerizatsii pod vozdejstviem oblucheniya; Estudios sobre los procesos primarios en la polimerizacion radioinducida

    Energy Technology Data Exchange (ETDEWEB)

    Okamura, S; Manabe, T; Higashimura, T; Oishi, Y; Futami, S

    1960-07-15

    styrolene amorcee au moyen de rayons gamma dans une solution de dichlorure de methylene a basse temperature. Le rapport direct constate entre la vitesse de polymerisation et l'intensite repond a une caracteristique du mecanisme de polymerisation ionique. La composition du copolymere obtenu avec le methacrylate de methyle vient egalement a l'appui de cette hypothese. (author) [Spanish] Observando la extincion de la luminiscencia del terienilo inducida por los rayos gamma, se ha estudiado el proceso de transmision de la energia de excitacion en soluciones bencenicas de monomeros, polimeros y copolimeros. La facilidad con que la energia se transmite del benceno a los diversos monomeros responde al siguiente orden decreciente: monomeros conjugados > monomeros no conjugados > compuestos saturados correspondientes. Tambien se realizaron experimentos sobre la extincion de la luminiscencia utilizando polimeros disueltos en benceno. El poder de extincion de los polimeros disminuye con arreglo al siguiente orden: caucho natural > poliestireno > acetato de polivinilo > polimeros acrilicos. Los resultados obtenidos al emplear un copolimero de estireno-metacrilato de metilo indicaron que el poder extintor especifico de los grupos estireno del copolimero es inferior al observado en el caso del homopolimero. La polimerizacion inducida por rayos gamma del estireno disuelto en diclorometileno tambien se estudio a bajas temperaturas. Los resultados obtenidos en lo que se refiere a la relacion existente entre la velocidad de polimerizacion y la intensidad de irradiacion parecen indicar que el mecanismo es de naturaleza ionica. La composicion del copolimero obtenido a partir de metacrilato de metilo tambien corrobora esta opinion. (author) [Russian] Peredacha ehnergii vozbuzhdeniya v benzol'nykh rastvorakh monomerov, polimerov i sopolimerov izuchayutsya putem nablyudeniya kvenchinga trifinil'noj fluorestsentsii, poluchaemogo v rezul'tate gamma-oblucheniya. Sposobnost' peredachi ehnergii ot

  5. Development and implementation of tPA clot lysis activity assay using ACL TOP™ hemeostasis testing system in QC laboratories

    Directory of Open Access Journals (Sweden)

    Lichun Huang

    2017-12-01

    Full Text Available This report describes the design, development, validation and long-term performance of tPA clot lysis activity assay using Advanced Chemistry Line Total Operational Performance (ACL TOP™ Homeostasis Testing System. The results of the study demonstrated robust and stable performance of the analytical method. The accuracy of the assay, expressed by percent recovery is 98–99%. The intermediate precision and repeatability precision, expressed as Relative Standard Deviation (RSD, was 3% and less than 2% respectively. The validated range is from 70% to 130% of the target potency of 5.8 × 105 IU/mg. The linearity of this range, expressed in correlation coefficient, is 0.997. After the assay is transferred to a QC laboratory, the assay retained high accuracy and precision with a success rate of >99%. Keywords: Potency assay, Clot lysis, Comparability, Automation

  6. Process

    Directory of Open Access Journals (Sweden)

    Eleanor T. Campbell

    2008-01-01

    Full Text Available El propósito de este estudio fue obtener una más clara comprensión del proceso de toma de decisiones en estudiantes de Enfermería que van a recibir su licenciatura, durante el cuidado de los pacientes. Se empleó un diseño descriptivo cualitativo, basado en preguntas abiertas semi-estructuradas, con el fin de recolectar información acerca de las decisiones, culturalmente diversas, tomadas por las estudiantes de Enfermería durante el cuidado de pacientes adultos, en una variedad de situaciones clínicas. Los temas que surgieron incluyen capacidad de comunicación, conocimiento, heroísmo, ansiedad y frustración. Los descubrimientos hallados indican que los estudiantes se propusieron metas en forma recíproca a todo lo largo del proceso de toma de decisiones. El autor empleó la teoría de la consecución de metas, de Imogene King, para interpretar los descubrimientos de este estudio y relacionar la teoría con la práctica. La toma de decisiones clínicas es un punto importante dentro de la educación y práctica de la Enfermería. La Facultad, entendiendo qué son los procesos de pensamiento, se anticipa con un diseño de estrategias específicas que refuerzan el razonamiento en la práctica clínica. Una parte de este proceso es ayudar a los estudiantes a salir de la dependencia que generan cuando quieren memorizar la información contenida en los textos, y hacerlos avanzar hacia la aplicación de los conceptos provenientes, tanto de las artes liberales, como de las ciencias biológicas y de Enfermería. Se trata de un especial desafío para las facultades y personal docente de Enfermería encargado de impartir educación a los estudiantes de la carrera, así como para el recién egresados de otras áreas de formación y de experiencia. Los resultados de estudios anteriores sobre toma de decisiones clínicas presentan conclusiones conflictivas e inconclusas, concernientes a determinadas consecuencias predecibles y otros factores

  7. El Impacto de los Procedos de Producción en el Rendimiento de la PYME Manufacturera de México: un Estudio Empírico (The impact of manufacturing processes on the performance of manufacturing SME´S in Mexico: an empirical study

    Directory of Open Access Journals (Sweden)

    María del Carmen Martínez Serna

    2012-12-01

    Full Text Available Los inicios del nuevo siglo se caracterizan por un alto desarrollo de la maquinaria y equipo, así como por la innovación y la tecnología, lo que ha transformado los medios de manufactura tradicionales en sistemas de manufactura flexibles, con la finalidad de mantener o adquirir una ventaja competitiva. En este sentido, este estudio analiza los resultados de una encuesta aplicada a 125 Pymes manufactureras de Aguascalientes (México para conocer los efectos que ejercen los procesos de producción en el rendimiento de las Pymes. La literatura analizada identifica tres actividades o factores básicos con los que se pueden medir los procesos de producción: automatización del proceso, confiabilidad del proceso y control administrativo. Así, los resultados obtenidos muestran que tanto el proceso de automatización como la confiabilidad del proceso y el control administrativo afectan positivamente el rendimiento de las Pymes.   ABSTRACT The beginning of the 21st. century is marked by an outstanding development of machinery and equipment, innovation, and technology that transforms traditional manufacturing methods into flexible manufacturing systems, aiming at acquiring or maintaining a competitive advantage. This work analyzes results of a survey practiced on 125 manufacturing SME´s from Aguascalientes (Mexico, aiming to understand the effect of production processes in SME performance. The literature researched identifies three basic activities or factors used to measure productive processes: process automation, process reliability and managerial control. The result shows that process automation, as well as process reliability and managerial control, positively affect SME performance.

  8. Soil pretreatment and fast cell lysis for direct polymerase chain reaction from forest soils for terminal restriction fragment length polymorphism analysis of fungal communities

    Directory of Open Access Journals (Sweden)

    Fei Cheng

    Full Text Available Abstract Humic substances in soil DNA samples can influence the assessment of microbial diversity and community composition. Using multiple steps during or after cell lysis adds expenses, is time-consuming, and causes DNA loss. A pretreatment of soil samples and a single step DNA extraction may improve experimental results. In order to optimize a protocol for obtaining high purity DNA from soil microbiota, five prewashing agents were compared in terms of their efficiency and effectiveness in removing soil contaminants. Residual contaminants were precipitated by adding 0.6 mL of 0.5 M CaCl2. Four cell lysis methods were applied to test their compatibility with the pretreatment (prewashing + Ca2+ flocculation and to ultimately identify the optimal cell lysis method for analyzing fungal communities in forest soils. The results showed that pretreatment with TNP + Triton X-100 + skim milk (100 mM Tris, 100 mM Na4P2O7, 1% polyvinylpyrrolidone, 100 mM NaCl, 0.05% Triton X-100, 4% skim milk, pH 10.0 removed most soil humic contaminants. When the pretreatment was combined with Ca2+ flocculation, the purity of all soil DNA samples was further improved. DNA samples obtained by the fast glass bead-beating method (MethodFGB had the highest purity. The resulting DNA was successfully used, without further purification steps, as a template for polymerase chain reaction targeting fungal internal transcribed spacer regions. The results obtained by terminal restriction fragment length polymorphism analysis indicated that the MethodFGB revealed greater fungal diversity and more distinctive community structure compared with the other methods tested. Our study provides a protocol for fungal cell lysis in soil, which is fast, convenient, and effective for analyzing fungal communities in forest soils.

  9. Comparison of the Lysis Centrifugation Method with the Conventional Blood Culture Method in Cases of Sepsis in a Tertiary Care Hospital

    OpenAIRE

    Parikh, Harshal R; De, Anuradha S; Baveja, Sujata M

    2012-01-01

    Introduction : Physicians and microbiologists have long recognized that the presence of living microorganisms in the blood of a patient carries with it considerable morbidity and mortality. Hence, blood cultures have become critically important and frequently performed test in clinical microbiology laboratories for diagnosis of sepsis. Objectives: To compare the conventional blood culture method with the lysis centrifugation method in cases of sepsis. Materials and Methods: Two hundred ...

  10. Hyperphosphatemia during spontaneous tumor lysis syndrome culminate in severe hyphosphatemia at the time of blast crisis of Phneg CML to acute myelomoncytic leukemia

    Directory of Open Access Journals (Sweden)

    Salomon Ophira

    2012-08-01

    Full Text Available Abstract Extreme swing of phosphor from severe hyperphosphatemia to severe hypophosphatemia in a patient with blast crisis of myeloid origin was the result of imbalance between massive apoptosis of leukemic cells in the context of spontaneous tumor lysis syndrome and massive production of leukemic cells with only 1% of blast in peripheral blood. The mutated p53 protein suggested acting as oncogene in the presented case and possibly affecting phosphor status.

  11. Reduction of excess sludge in a sequencing batch reactor by lysis-cryptic growth using quick lime for disintegration under low temperature.

    Science.gov (United States)

    Lv, Xiao-Mei; Song, Ju-Sheng; Li, Ji; Zhai, Kun

    2017-08-01

    In the present study, quick-lime-based thermal-alkaline sludge disintegration (SD) under low temperature was combined with cryptic growth to investigate the excess sludge reduction efficiency in the sequencing batch reactor (SBR). The optimized condition of SD was as follows: T = 80℃, pH = 11, t = 180 min, and the SD rate was about 42.1%. With 65.6% of excess sludge disintegrated and returned to the SBR, the system achieved sludge reduction rate of about 40.1%. The lysis-cryptic growth still obtained satisfactory sludge reduction efficiency despite the comparative low SD rate, which suggested that disintegration rate might not be the decisive factor for cryptic-growth-based sludge reduction. Lysis-cryptic growth did not impact the effluent quality, yet the phosphorus removal performance was enhanced, with effluent total phosphorus concentration decreased by 0.3 mg/L (33%). Crystal compounds of calcium phosphate precipitate were detected in the system by Fourier transform infrared spectroscopy and X-ray diffraction, which indicated the phosphorus removal potential of SD using lime. Moreover, endogenous dehydrogenase activity of activated sludge in the lysis-cryptic system was enhanced, which was beneficial for sludge reduction. SD and cryptic growth in the present study demonstrates an economical and effective approach for sludge reduction.

  12. Leukocyte function-associated antigen-1-dependent lysis of Fas+ (CD95+/Apo-1+) innocent bystanders by antigen-specific CD8+ CTL.

    Science.gov (United States)

    Kojima, H; Eshima, K; Takayama, H; Sitkovsky, M V

    1997-09-15

    Exquisite specificity toward Ag-bearing cells (cognate targets) is one of the most important properties of CD8+ CTL-mediated cytotoxicity. Using highly Ag-specific CD8+ CTL lines and clones, which spare noncognate, Ag-free targets, we found that in the presence of Ag-bearing targets the CTL acquire the ability to lyse noncognate target cells (bystanders). It is shown that the unexpectedly rapid and efficient lysis of bystanders by Ag-activated CTL is mediated by a Fas ligand (FasL)/Fas-based mechanism and does not depend on perforin. The CTL lysed Fas-expressing bystanders, but spared the Fas-negative or anti-Fas mAb-resistant bystander cells. Accordingly, the FasL-deficient gld/gld CTL did not kill bystanders, while perforin-deficient CTL did. Unlike anti-Fas mAb-induced cell death, the lysis of bystanders was not only FasL/Fas dependent but also required adhesion molecule LFA-1 on the surface of the activated CTL. Lysis of bystanders is viewed as acceptable "collateral" damage, but the persistent presence of activated CTL could result in immunopathologies involving functional Fas-expressing tissues.

  13. Demonstration of NK cell-mediated lysis of varicella-zoster virus (VZV)-infected cells: characterization of the effector cells

    Energy Technology Data Exchange (ETDEWEB)

    Tilden, A.B.; Cauda, R.; Grossi, C.E.; Balch, C.M.; Lakeman, A.D.; Whitley, R.J.

    1986-06-01

    Infection with varicella-zoster virus (VZV) rendered RAJI cells more susceptible to lysis by non-adherent blood lymphocytes. At an effector to target ratio of 80:1 the mean percentage of /sup 51/Cr release of VZV-infected RAJI cells was 41 +/- 12%, whereas that of uninfected RAJI cells was 15 +/- 6%. The increased susceptibility to lysis was associated with increased effector to target conjugate formation in immunofluorescence binding assays. The effector cells cytotoxic for VZV-infected RAJI cells were predominantly Leu-11a/sup +/ Leu-4/sup -/ granular lymphocytes as demonstrated by fluorescence-activated cell sorting. The effector cell active against VZV-infected RAJI cells appeared similar to those active against herpes simplex virus (HSV)-infected cells, because in cold target competition experiments the lysis of /sup 51/Cr-labeled VZV-infected RAJI cells was efficiently inhibited by either unlabeled VZV-infected RAJI cells (mean 71% inhibition, 2:1 ratio unlabeled to labeled target) or HSV-infected RAJI cells (mean 69% inhibition) but not by uninfected RAJI cells (mean 10% inhibition). In contrast, competition experiments revealed donor heterogeneity in the overlap between effector cells for VZV- or HSV-infected RAJI vs K-562 cells.

  14. Study of hypercharge exchange processes 0{sup -}1/2+{yields}1{sup -} 1/2+; Estudio de procesos 0-1/2+{yields}1-1/2+ con intercambio de hipercarga

    Energy Technology Data Exchange (ETDEWEB)

    Albajar Molera, M c; Aguilar Beniter de Lugo, M.

    1981-07-01

    In this work we present a formalism for the reconstruction of the transitivity amplitudes governing the processes of the type 0-1/2+{yields}1-1/2+. The formalism uses the information contained in the decay angular correlations and takes into account the existence of mixed spin configurations in the final state 0-1/2+{yields}(0{down_arrow}, 1-)1/2+ (Author) 10 refs.

  15. Troquel sensorizado para el estudio de las principales fuerzas que intervienen en el proceso de embutición. // Sensitivity Die for the study of the main forces in the rivet process.

    Directory of Open Access Journals (Sweden)

    L. A. Valdivia Pérez

    2006-05-01

    Full Text Available El control de las fuerzas que intervienen en el desarrollo del embutido, así como también en la generación de la fuerza defricción, es una vía para lograr la disminución de los defectos en las piezas y minimizar la fricción. Mediante el empleo dela electrónica moderna y las técnicas de sensorización, como complemento necesario, puede obtenerse un control integraldel proceso.En el presente trabajo se muestra el proceso de diseño y selección de los componentes de un sistema de regulación ycontrol para un troquel que ha sido diseñado y construido con el objetivo de conocer el comportamiento de las principalesfuerzas que intervienen en el proceso de embutición. Como elementos de medición y control de los parámetros del procesose utilizan diversos tipos de sensores o captadores.Palabras claves: Control de fuerzas en el embutido, troquel sensorizado, sistema de control, captador, fricción ydesgaste en troqueles.____________________________________________________________________________Abstract.The control of forces that intervene in the development of the deep drawing process, as well as in the generation of thefriction force it is a way to achieve the decrease of defects in spares, and to minimize friction.The present paper deals with the process of design and selection of components of the regulation and control system for adie designed and build for the analysis of the principal forces in the deep drawing process. Different types of sensors orcollectors have been used in order to allow the control and the measurement of parameters.Keywords: Deep drawing process, control system, captator, friction and wear in die.

  16. Flocculation-coagulation behaviour study of tailings of a fluorspar mineral processing plant; Estudio del comportamiento de las colas de una planta de beneficio de mineral de fluorita frente a procesos floculacion-coagulacion

    Energy Technology Data Exchange (ETDEWEB)

    Dzioba, B. R.; Diaz, A. A.; Menendez-Aguado, J. M.

    2004-07-01

    The objective of this work is to carry out a selection study of the most adequate flocculation-coagulation reagents to reduce the solids content in the overflow from the settling lagoons of a fluorspar processing plant. This overflow has 1/ solids content, clarified from a feed of 25% solids in the settling lagoons, and is pilled into a river. The importance of this work is enhanced by the fact that the plant is located in a low water resources area, and that eventually the water from the river is used for agriculture or even human consumption. It is relevant to find a working methodology which allow to improve the efficiency of the clarifying process and minimize the environmental impact. Regarding the research methodology, a series of trials were made at natural pH and 25 degree centigree to evaluate the action of those reagents which previously probed to be more efficient in this case: as flocculants were tried BOZEFLOC C 65, SEPARAN AP 273 P. SEPARAN MG 200, and as coagulant aluminium sulfate. The possible interaction of flocculation-coagulation processes was also studied, to obtain optimum result. As analysis criterium the total flocculation time was used, and as all essays were made in 100 ml probes, it corresponds with flocculation rate. As result of this study it can be concluded that the most efficient floculant was SEPARAN MG 200, at 4 g/T. with the observation that an excess of aluminium sulfate gets down the efficiency. (Author) 6 refs.

  17. Geochemical study of water-rock interaction processes on geothermal systems of alkaline water in granitic massif; Estudio geoquimico de los procesos de interaccion agua-roca sobre sistemas goetermales de aguas alcalinas en granitoides

    Energy Technology Data Exchange (ETDEWEB)

    Buil gutierrez, B; Garcia Sanz, S; Lago San Jose, M; Arranz Uague, E; Auque Sanz, L [Universidad de Zaragoza (Spain)

    2002-07-01

    The study of geothermal systems developed within granitic massifs (with alkaline waters and reducing ORP values) is a topic of increasing scientific interest. These systems are a perfect natural laboratory for studying the water-rock interaction processes as they are defined by three main features: 1) long residence time of water within the system, 2) temperature in the reservoir high enough to favour reaction kinetics and finally, 3) the comparison of the chemistry of the incoming and outgoing waters of the system allows for the evaluation of the processes that have modified the water chemistry and its signature, The four geothermal systems considered in this paper are developed within granitic massifs of the Spanish Central Pyrenes; these systems were studied from a geochemical point of view, defining the major, trace and REE chemistry of both waters and host rocks and then characterizing the composition and geochemical evolution of the different waters. Bicarbonate-chloride-sodic and bicarbonate-sodic compositions are the most representative of the water chemistry in the deep geothermal system, as they are not affected by secondary processes (mixing, conductive cooling, etc). (Author)

  18. Physicochemical studies on sunflower oil blended with cold pressed tiger nut oil during the deep frying process; Estudios fisicoquimicos sobre mezclas de aceite de girasol con aceite de chufa prensado en frio durante el proceso de fritura

    Energy Technology Data Exchange (ETDEWEB)

    Al Rehah, F. M.; Anany, A. M.

    2012-07-01

    Sunflower oils were blended with different levels of cold pressed tiger nut oil. Blended oils were obtained by mixing tiger nut oil with sunflower oil at the volume ratios of 0:100, 10: 90, 20: 80, 30: 70, 40: 60, 50:50 and 100: 0. The effects of deep frying on physico-chemical parameters (Free Fatty Acid (FFA), Peroxide Value (PV), thiobarbituric acid value (TBA), iodine value, Total Polar Compounds (TPC), color and viscosity) were evaluated over 30 hours of the frying process. The total phenolic content of native oils was determined. GLC analysis was performed to illustrate the fatty acid composition of sunflower oil, tiger nut oil and binary mixtures of them as well as their oxidation rates. The pure and blended oils were heated at 180 degree centigrade {+-} 5 degree centigrade, then frozen French fried potatoes were fried every 30 min. Oil samples were taken every 5 h and the entire continuous frying period was 30 h. The results showed that fresh sunflower oil had significantly the highest value of COX (7.25); while tiger nut oil had significantly the lowest (2.24). Mixing sunflower oil with different levels of tiger nut oil led to an increase in its stability against oxidation. The phenolic content of cold pressed tiger nut oil was about 3.3 times as high as that of sunflower oil. The analytical data showed that the lowest deterioration during the frying process occurred in tiger nut oil and the highest in sunflower. The changes in the physico-chemical parameters were controlled and significantly (P < 0.05) decreased when tiger nut /sunflower oil (W/W) proportions were varied between 20/80 to 50/50. The obtained results indicate that mixing sunflower oil with cold pressed tiger nut oil increased the stability and hence improved the quality of sunflower oil during the frying process. (Author) 68 refs.

  19. Comparative analysis of triangular rigid zone models in the mechanical study of drawing processes by upper bound; Analisis compartivo de modelos de bloques rigidos triangulares en el estudio mecanico de procesos de estirado por limite superior

    Energy Technology Data Exchange (ETDEWEB)

    Rubio, E. M.; Domingo, R.; Gonzalez, C.; Sanz, A.

    2004-07-01

    To study the optimised geometrical configuration to carry out mechanical drawing of plates is the main objective of this paper. To accomplish this objective, a comparative analysis of some suitable geometrical and kinematic configurations of the material located in the deformation zone has been made. Concretely, several triangular rigid zone models have been chosen, for each one, the overall energy involved in the process have been calculated and an estimation of its different components has been made. The calculation of the energy has been achieved applying the Upper Bound Theorem under plane strain and partial friction conditions. In addition, the range of use for the selected configurations has been established. (Author) 19 refs.

  20. Influence of the physico-chemical process as previous treatment to the electrocoagulation. Study with leachate of a landfill; Influencia del proceso fisicoquimico como tratamiento previo a la electrocoagulacion. Estudio con lixiviados de un relleno

    Energy Technology Data Exchange (ETDEWEB)

    Mercado Martinez, I. D.; Reyes Avila, D.

    2007-07-01

    This article has as purpose to show the influence of the method physico-chemical as previous process to the novel technique of electrocoagulation, presenting as a new alternative to depurate wastewaters. to obtain it, was done tests measuring the very parameters to leachates so much affluent as effluent from treatment plant of the Sanitary Landfill La Esmeralda, situated near to the Manizales city(Colombia), and so much before as later to apply this electrochemical technology, looking for this way to compare the obtained removals. (Author)

  1. Viability study on using calcium carbonate for the boron adsorption process in waste waters; Estudio de viabilidad del proceso de adsorcion de boro de aguas residuales con carbonato calcico

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez Guerreiro, M. J.; Munoz Camacho, E.; Bernal Pita da Veiga, M. B.

    2009-07-01

    This study evaluates how viable it is to employ calcium carbonate for the boron adsorption process in waters that could be contaminated by this element. A residue form mussel shells-abundant in Galicia, northwestern Spain, was used. The data gathered from the experiments show that the performance of the boron adsorption within the sample is below 2%. Despite the inferior data obtained, the general aim was reached. An attempt was made to find solutions to the environmental problem caused by the residues mentioned above. (Author) 11 refs.

  2. El creciente campo de los Estudios Sensoriales

    Directory of Open Access Journals (Sweden)

    David Howes

    2014-09-01

    Full Text Available Este ensayo presenta una breve descripción acerca del giro sensorial en la investigación contemporánea, y propone algunas perspectivas de trabajo para futuras investigaciones. Esta labor no pretende ser exhaustiva y, más bien, busca indicar las principales tendencias en este campo. El ensayo, en su primera parte, ofrece una mirada general sobre la aparición y el desarrollo de la historia y la antropología de los sentidos. En la segunda parte, la atención se concentra en cómo el campo de los estudios sensoriales puede ser, de otro lado, conceptualizado como compuesto de cultura visual, cultura auditiva (o estudios del sonido, cultura del olfato, cultura del gusto y cultura del tacto. Se ofrece una explicación acerca de la génesis de estas divisiones. Posteriormente, se presenta una visión general de algunas cuestiones claves en los estudios sensoriales, como la pregunta por el número de los sentidos y la relación entre orden sensorial y orden social. El ensayo concluye con ocho proposiciones para los estudios sensoriales.

  3. El creciente campo de los Estudios Sensoriales

    Directory of Open Access Journals (Sweden)

    David Howes

    2014-08-01

    Full Text Available Este ensayo presenta una breve descripción acerca del giro sensorial en la investigación contemporánea, y propone algunas perspectivas de trabajo para futurasinvestigaciones. Esta labor no pretende ser exhaustiva y, más bien, busca indicar las principales tendencias en este campo. El ensayo, en su primera parte, ofrece una mirada general sobre la aparición y el desarrollo de la historia y la antropología de los sentidos. En la segunda parte, la atención se concentra en cómo el campo de los estudios sensoriales puede ser, de otro lado, conceptualizado como compuesto de cultura visual, cultura auditiva (o estudios del sonido, cultura del olfato, cultura del gusto y cultura del tacto. Se ofrece una explicación acerca de la génesis de estas divisiones. Posteriormente,se presenta una visión general de algunas cuestiones claves en los estudios sensoriales, como la pregunta por el número de los sentidos y la relación entre orden sensorial y orden social. El ensayo concluye con ocho proposiciones para los estudios sensoriales.

  4. Estudio del proceso de génesis instrumental del artefacto simbólico función exponencial Study of the process of instrumental genesis of the symbolic artifact for the exponential function

    Directory of Open Access Journals (Sweden)

    Mihály André Martínez Miraval

    2018-05-01

    Full Text Available This article describes a study of the exponential function on students of the first semester of a private University in Lima, Peru. The research was aimed at analyzing the Instrumental genesis process of this mathematical object assisted by the use of Geogebra software. Rabardel's Instrumental Approach was used as a theoretical framework. Likewise, some notions of Artigue's Didactic Engineering were used as a methodology, focusing on a priori and a posteriori analysis. The students’ answers allowed to identify the use of schemes built and activated, while interacting with the symbolic artifact for the exponential function. The use of Geogebra favored the instrumentalization and instrumentation of the addressed mathematical object since it allowed the students to validate their conjectures. Keywords:

  5. Síndrome de lise tumoral: uma revisão abrangente da literatura Acute tumor lysis syndrome: a comprehensive review

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    Michael Darmon

    2008-09-01

    égias baseadas no risco dos pacientes são necessários para limitar a alta morbidade e mortalidade desta complicação.Tumor lysis syndrome is characterized by the massive destruction of malignant cells and the release in the extra-cellular space of their content. While Tumor lysis syndrome may occur spontaneously before treatment, it usually develops shortly after the initiation of cytotoxic chemotherapy. These metabolites can overwhelm the homeostatic mechanisms with development of hyperuricaemia, hyperkalaemia, hyperphosphataemia, and hypocalcaemia. These biological manifestations may lead to clinical manifestations including, acute kidney injury, seizure, or sudden death that require intensive care. Since clinical tumor lysis syndrome is associated with a poor prognosis both prevention of tumor lysis syndrome and prevention of clinical consequences of tumor lysis syndrome are mandatory. The objective of this review is to describe pathophysiological mechanisms, biological and clinical manifestations of tumor Lysis syndrome, and to provide upto-date guidelines to ensure prevention of tumor lysis syndrome. Review of selected studies on tumor lysis syndrome published at the PubMed database www.pubmed.gov during the last 20 years. Additional references were retrieved from the studies initially selected. Tumor lysis syndrome is a frequent and life-threatening complication of the newly diagnosed malignancies. Preventive measures, including hydration, uricolytic agents, eviction of factors predisposing to acute kidney injury and, in the more severe patients, on prophylactic renal replacement therapy, are required to prevent or limit clinical consequences of Tumor lysis syndrome. However optimal timing and modalities of prevention remains unknown and may be modified by the changing spectrum of patients at risk of tumor lysis syndrome. Development and validation of risk based strategies is required to limit the high morbidity and mortality of this complication.

  6. Hyperuricemia and tumor lysis syndrome in children with non-Hodgkin’s lymphoma and acute lymphoblastic leukemia

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    Betül Sevinir

    2011-03-01

    Full Text Available Objective: This study aimed to examine the incidence, clinical characteristics, and outcome of hyperuricemia and tumor lysis syndrome (TLS in children with non-Hodgkin’s lymphoma (NHL and acute lymphoblastic leukemia (ALL.Materials and Methods: This retrospective study included data from 327 patients (113 NHL and 214 ALL.Results: Hyperuricemia occurred in 26.5% and 12.6% of the patients with NHL and ALL, respectively. The corresponding figures for TLS were 15.9% and 0.47% (p=0.001. All hyperuricemic NHL patients had advanced disease and renal involvement was present in 53%. All hyperuricemic ALL patients had a leukocyte count >50,000 mm3 at the time of diagnosis. Among the hyperuricemic NHL and ALL patients, 96.6% and 66.6% had LDH ≥500 UI/L, respectively. Treatment consisted of hydration and allopurinol; none of the patients received urate oxidase. Among the patients that developed TLS, 26.3% had laboratory TLS, 42.1% had grade I or II TLS, and 31.6% had grade III or IV TLS. Uric acid levels returned to normal after a mean period of 3.5±2.5 and 3.05±0.8 d in NHL and ALL groups, respectively. In all, 7% of the patients with hyperuricemia required hemodialysis. None of the patients died.Conclusion: In this series the factors associated with a high-risk for TLS were renal involvement in NHL and high leucocyte count in ALL. Management with allopurinol and hydration was effective in this group of patients with high tumor burden.

  7. Genome scan of clot lysis time and its association with thrombosis in a protein C-deficient kindred.

    Science.gov (United States)

    Meltzer, M E; Hasstedt, S J; Vossen, C Y; Callas, P W; DE Groot, Ph G; Rosendaal, F R; Lisman, T; Bovill, E G

    2011-07-01

     Previously, we found increased clot-lysis time (CLT), as measured with a plasma-based assay, to increase the risk of venous thrombosis in two population-based case-control studies. The genes influencing CLT are as yet unknown.  We tested CLT as risk factor for venous thrombosis in Kindred Vermont II (n = 346), a pedigree suffering from a high thrombosis risk, partially attributable to a type I protein C deficiency. Furthermore, we tested for quantitative trait loci (QTLs) for CLT, using variance component linkage analysis.  Protein C-deficient family members had shorter CLTs than non-deficient members (median CLT 67 min vs. 75 min). One standard deviation increase in CLT increased the risk of venous thrombosis 2.4-fold in non-deficient family members. Protein C deficiency without elevated CLT increased the risk 6.9-fold. Combining both risk factors yielded a 27.8-fold increased risk. The heritability of CLT was 42-52%. We found suggestive evidence of linkage on chromosome 11 (62 cM), partly explained by the prothrombin 20210A mutation, and on chromosome 13 (52 cM). Thrombin-activatable fibrinolysis inhibitor genotypes did not explain the variation in CLT. Hypofibrinolysis appears to increase thrombosis risk in this family, especially in combination with protein C deficiency. Protein C deficiency is associated with short CLT. CLT is partly genetically regulated. Suggestive QTLs were found on chromosomes 11 and 13. © 2011 International Society on Thrombosis and Haemostasis.

  8. Host lysozyme-mediated lysis of Lactococcus lactis facilitates delivery of colitis-attenuating superoxide dismutase to inflamed colons

    Science.gov (United States)

    Ballal, Sonia A.; Veiga, Patrick; Fenn, Kathrin; Michaud, Monia; Kim, Jason H.; Gallini, Carey Ann; Glickman, Jonathan N.; Quéré, Gaëlle; Garault, Peggy; Béal, Chloé; Derrien, Muriel; Courtin, Pascal; Kulakauskas, Saulius; Chapot-Chartier, Marie-Pierre; van Hylckama Vlieg, Johan; Garrett, Wendy S.

    2015-01-01

    Beneficial microbes that target molecules and pathways, such as oxidative stress, which can negatively affect both host and microbiota, may hold promise as an inflammatory bowel disease therapy. Prior work showed that a five-strain fermented milk product (FMP) improved colitis in T-bet−/− Rag2−/− mice. By varying the number of strains used in the FMP, we found that Lactococcus lactis I-1631 was sufficient to ameliorate colitis. Using comparative genomic analyses, we identified genes unique to L. lactis I-1631 involved in oxygen respiration. Respiration of oxygen results in reactive oxygen species (ROS) generation. Also, ROS are produced at high levels during intestinal inflammation and cause tissue damage. L. lactis I-1631 possesses genes encoding enzymes that detoxify ROS, such as superoxide dismutase (SodA). Thus, we hypothesized that lactococcal SodA played a role in attenuating colitis. Inactivation of the sodA gene abolished L. lactis I-1631’s beneficial effect in the T-bet−/− Rag2−/− model. Similar effects were obtained in two additional colonic inflammation models, Il10−/− mice and dextran sulfate sodium-treated mice. Efforts to understand how a lipophobic superoxide anion (O2−) can be detoxified by cytoplasmic lactoccocal SodA led to the finding that host antimicrobial-mediated lysis is a prerequisite for SodA release and SodA’s extracytoplasmic O2− scavenging. L. lactis I-1631 may represent a promising vehicle to deliver antioxidant, colitis-attenuating SodA to the inflamed intestinal mucosa, and host antimicrobials may play a critical role in mediating SodA’s bioaccessibility. PMID:26056274

  9. DEPENDENCE BETWEEN ACYLATION DEGREE AND SPECIFIC LYSIS ACTITIVY OF THE PSEUDOMONAS AERUGINOSA М6 BACTERIOPHAGE

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    Martynov A. V.

    2016-06-01

    Full Text Available Introduction. The purpose of this work is to study the influence of various degrees of protein’s acylation on lytic activity and other biological properties of Pseudomonas M6 bacteriophage. Materials and methods. The subject of the study were samples of the Pseudomonas O 12 bacteriophage, hereinafter named the M6 phage. Pure phage lines were obtained from individual standard-morphology phage plaques (plaque-forming units, or PFUs after ten passes over the indicator strain. In subsequent experiments, M6 phage samples were selected that had a sufficient level of purity (homogeneous in the morphology of virions that inactivated the homologic antiphage serum by no less than 99.0% and the heterologic antibacterial serum by no more than 1%, and a titer no lower than 10 PFU per ml (PFU/ml. Titration of the phages was conducted using the standard bilayer agar. The crude protein content in the phage suspensions was determined using the spectroscopic method (at 280 and 260 nm. Succinylation of the phage samples was conducted according to the method developed by T.J. Molenaar. The phage’s lytic activity spectrum was determined through spot tests on 224 strains of the Pseudomonas genus and 106 strains of other genera (correspondingly, of strains Enterobacter -40, Escherichia -10, Citrobacter -8, Hafnia -7, Serratia -6, Shigella -10, Salmonella -8, Proteus -7 through the use of a phage suspension containing 10 5 - 10 6 PFU/ml. (This concentration of infectious phage corpuscles corresponds with the critical distribution of the M6 phage. The results of the research were subjected to statistical processing using single-factor dispersion analysis. Results and discussion. One hundred percent phage protein acylation leads to a loss of adsorption ability on sensitive cells and is accompanied by a significant (more than 103 times decrease in the level of specific lytic activity in the phage preparations. The succinylation type being studied does not cause

  10. Study in stationary state of the subcriticality of intermediate configurations of core in the reloading process of a BWR; Estudio en estado estacionario de la subcriticidad de configuraciones intermedias de nucleo en el proceso de recarga de un BWR

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez, J.L.; Montes, J.L.; Perusquia, R.; Ortiz, J.J. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)]. e-mail: jlhm@nuclear.inin.mx

    2006-07-01

    In this work is carried out the simulation in three dimensions with the COREMASTER-PRESTO code, of the behavior of the reactor core in different stages of the change process of fuel assemblies. To carry out the simulation, this code requires of a database of nuclear parameters that includes those that can associate to the areas of an assemblies that they don't contain fuel and in its place there is moderator. These nuclear parameters are calculated with the AURORA-HELIOS-ZENITH-TABGEN system. One of the approaches that were carried out consisted on designing a 'water assemble', that is to say, an axial arrangement of 25 'water cells'. To obtain the appropriate 'water cell' its were carried out some selective test cases, since it presents in two cases the necessity to find an enough minimum value of fissile material for the correct execution of HELIOS, firstly, and later on COREMASTER-PRESTO. In the first case, the situation is solved when placing symmetrically 6 bars with natural uranium in the lateral areas of the cell; with that which the value of k{sub inf} of 0.1592 is obtained in the calculations with the HELIOS code in the cold condition to zero power (CZP), and 0% of vacuums. For the second case the cell includes symmetrically 28 bars with natural uranium, and the k{sub inf} value is 0.45290. These values are the maximum through the life of the 'cell.' As part of the activities that are developed during the fuel substitution, this the one of evaluating the subcriticality of the core each determined number of substitution movements. The obtained results when evaluating the k-effective in cold condition, in 5 different intermediate core configurations, as the loading process of the fuel advances are presented. To make the evaluation with CM-PRESTO in each configuration it was proceeded to complete the rest of the 444 assemblies with the one denominated 'water assemble'. In all the evaluated cases the

  11. Taxonomy and Physiology of un-wanted bacterial flora in activated sludge process. Study in a pilot plant; Taxonomia y fisiologia de la flora bacteriana indeseable en el proceso de fangos activados. Estudio de una plant piloto

    Energy Technology Data Exchange (ETDEWEB)

    Berrocal Escobar, M.; Lopez Fernandez, C. L.; Arias Fernandez, M. E.; Perez Leblic, M. I.; Zapatero Martin, I.; Leton Garcia, P.; Garcia Calvo, E. [Universidad de Alcala de Henares. Madrid (Spain); Aznar Munoz, R.; Rodriguez Medina, P. [Departamento Tecnico y de Calidad de Seragua, S.A. Madrid (Spain)

    1998-12-31

    The activated sludge used in the wastewater depuration in treatment plants could be considered as an artificial microbial ecosystem in balance. In this community which is constituted by free and flocculated bacteria, protozoa, rotifers, nematodes and a few other invertebrates, the stability of the system is maintained by the continuous food competition. The breakdown of this stability due to a high proliferation of filametous bacteria drive to the phenomenon called bulking. Nowadays, to avoid bulking is one of the main objectives in research because is the main cause of the malfunction of wastewater depuration interfering with compaction, settling, thickening and, concentration of activated sludge. In the present work, a taxonomical and physiological study of the microbial community which carries out the cleaning of wastewater in an activated sludge system has been performed by using an airlift bioreactor working in continuous. Activated sludge coming from a conventional wastewater plant was used as inoculum (starter culture). The nutritional conditions and bioreactor system parameters in which the filamentous bacteria grow in excess have been established. Several of filamentous bacteria responsible for bulking have been identified: Sphaerotilus natans, type 021N, Nocardia spp., Microthrix parvicella, Thiotrix I, Thiotrix II, type 0803, type 0581, Nostocoida limicola I and III and, type 1863. In addition, protozoa of groups involved in the depuration process (free-swimming ciliates, attached ciliates, crawling ciliates, carnivorous ciliates, flagellates and amoebae) were observed as well as rotifer and nematode populations. (Author) 13 refs.

  12. Representación del conocimiento y percepción subjetiva del proceso de aprendizaje profesional: estudio cualitativo en personal de enfermería Knowledge representation and perception of the professional learning process: qualitative study in nursing

    Directory of Open Access Journals (Sweden)

    M. Jiménez-Adán

    2010-09-01

    Full Text Available Objetivo. Presentamos una investigación cualitativa cuyo objetivo fue conocer las percepciones de un grupo de enfermeras y enfermeros sobre su proceso de aprendizaje profesional. Materiales y métodos. Se utilizó una metodología de análisis de textos que combinó el análisis de contingencias y las Redes Asociativas Pathfinder. Resultados y conclusiones. Los resultados indican que el factor más importante del aprendizaje profesional es la relación personal y la ayuda de otros compañeros, no sólo durante la práctica profesional, sino también durante la formación inicial.Aim. We present a qualitative research whose goal was to determine the perceptions of a group of nurses about their professional learning process. Materials and methods. We used a text analysis methodology that combined contingency analysis and Pathfinder Associative Networks. Results and conclusions. The results indicate that the most important thing in professional learning is the personal relationship and the help of other colleagues, not only during practice, but also during initial training.

  13. Propuesta sobre el proceso productivo para financiar empresas del deporte a partir del estudio de sus seguidores = Proposal on the production process to finance companies sport from studying his followers

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    Wilson Andrés Cárdenas-Polanía

    2015-12-01

    Abstract The follower of sport should be the focus of the operation of sport companies. For that we must understand their needs so we can develop the business model that gives direction to the sport company to achieve financial sustainability. The proposal is based on social psychology and sociology at the theoretical part and in two methodological research: A transversal cross-correlation descriptive and the other is based on statistical analysis of textual data. Taking some of its conclusions, the proposal aimed to channel the needs of the follower of sports to an exchange called cultural consumption. For this, the necessary elements that the company must develop to maximize emotions, motivations and identities during games, tournaments and sporting events. It does this using the amplification of symbolic values, develop statistics and promote the practice of sport. With this tour, arise actors that the process for its success must accompany: Athletes, media and advertisers. With these inputs we can make an intersection between the follower and the consumer of the sponsor.   Keywords: sport marketing, fan of sports, business model, costumer needs [1] Profesor de la Facultad de Marketing en la Universidad de Santo Tomás (Bogotá-Colombia. Es Candidato a doctor en Administración de Empresas de la Universidad Católica de Argentina (UCA, Magister en Psicología del Consumidor de la Fundación Universitaria Konrad Lorenz (Colombia y Administrador de Empresas de la Pontificia Universidad Javeriana (Colombia. Ha trabajado en empresas del deporte desde 2000. Mail: proyectos.consultor@gmail.com  Twitter: @acardenas74 LinkedIn: co.linkedin.com/in/AndresCardenaspolania

  14. O processo de análise do ambiente e sua relação com a aprendizagem organizacional: um estudo de caso El proceso de análisis del medio ambiente y su relación con la aprendizaje en organizaciones: un caso de estudio The process of environment analysis and its relationship to organizational learning: a case study

    Directory of Open Access Journals (Sweden)

    Adilson Caldeira

    2012-12-01

    estrategia de negocios de hoy. En el plano empírico, se presenta una investigación cualitativa que hace un estudio de caso en Petrobras, en que los datos se recopilaron mediante entrevistas. En primer lugar, presentamos los datos históricos y la descripción del análisis ambiental realizado en el proceso de planificación estratégica en la organización estudiada. A continuación, se presentan las opiniones de los directivos en relación con el aprendizaje derivado del proceso. Las declaraciones de los ejecutivos fueron clasificados por categorías y sometidos a análisis de contenido. Los resultados muestran que los consultados entienden que el análisis del medio ambiente es un medio de aprendizaje en la organización y dicen que que el aprendizaje contribuye al rendimiento del negocio mediante el desarrollo de sus habilidades para mejorar su actuación administrativa. Así, el estudio proporciona la visión de una asociación entre los procesos de planificación estratégica y el aprendizaje en las organizaciones.This study considered the question: "Which are the contributions of environment analysis to organizational learning?" It principiates by means of a literature review that discusses the key concepts and applications related to ways in which learning occurs in organizations. Subsequently, a brief frame concerning to business strategy contextualizes the theoretical and conceptual studies that permeates the discussion of this matter presently. Empirically, a qualitative research was conducted by means of a single case study approach conducted at Petrobras, using interviews . After presenting the history of company and its strategic planning process, the study discusses how managers see their learning when analysing environment. The research reveals that managers see environment analysis as a way to provide learning. They also believe that outcoming learning contributes to company's performance by improving its management ability. The study provided a first look

  15. Casos de Estudio de Distribuciones de Probabilidad para Turismo

    OpenAIRE

    Fernández Morales, Antonio

    2016-01-01

    Este trabajo propone diversos casos de estudio para el estudio de las distribuciones de probabilidad aplicadas a la investigación y la práctica profesional en el ámbito del turismo. Se afronta el estudio de distribuciones de probabilidad, tanto de variables aleatorias discretas como continuas.

  16. Estudios de competitividad en sistemas urbano - territoriales

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    Esteban Soms García

    2007-05-01

    El Ministerio de Planificación y Cooperación de Chile, ha desarrollado varios estudios relacionados con la competitividad regional, destinados a pronosticar y evaluar los impactos positivos y negativos que podrían derivarse de los recientes Acuerdos de Libre Comercio suscritos por Chile con la Comunidad Europea, Estados Unidos y Corea, más los acuerdos ad portas con los Países de la APEC. En lo referente a competitividad urbana, destacan algunos estudios y proyectos relacionados con el programa gubernamental "Ciudades para el Bicentenario", impulsado el Ministerio de Vivienda y Urbanismo y los Gobiernos Regionales de Antofagasta, Bio Bio, Valparaíso y Santiago.

  17. Estrategia organizacional: una propuesta de estudio

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    Ángela Lucía Noguera Hidalgo

    2014-01-01

    Full Text Available El concepto de estrategia en el contexto de las organizaciones empresariales es uno de los temas que genera gran interés en los asuntos del management. Sin embargo, la proposición de nuevos enfoques no ha aportado significativamente al avance en el estudio de este concepto. El estancamiento se hace evidente, razón por la cual el presente documento esboza una propuesta que reúne algunos de los retos para el estudio de la estrategia. En él se presenta una revisión que deja por sentados los posibles caminos a seguir, los cuales contribuyen a la perdurabilidad de las organizaciones.

  18. What Variables Are Associated With the Outcome of Arthroscopic Lysis and Lavage Surgery for Internal Derangement of the Temporomandibular Joint?

    Science.gov (United States)

    Haeffs, Tyler H; D'Amato, Lindsay N; Khawaja, Shehryar N; Keith, David A; Scrivani, Steven J

    2018-04-26

    Arthroscopic lysis and lavage surgery (AS) is an effective modality that can decrease pain and increase maximum interincisal opening (MIO) in patients with internal derangement (ID) of the temporomandibular joint (TMJ). However, some patients remain in pain or have limited mandibular range of motion despite AS. The purpose of this study was to determine the effectiveness, prevalence of adverse effects, and predictors of response to TMJ AS in patients with TMJ arthralgia and ID. A retrospective cohort study was conducted using data of patients who had undergone AS by a single surgeon (D.A.K.) from September 2010 to April 2015 in the Department of Oral and Maxillofacial Surgery at Massachusetts General Hospital (Boston, MA). Variables, including demographic data, medical history, and clinical presentation, were extracted and analyzed. Criteria for surgical success were defined as a postoperative MIO of at least 35 mm and a postoperative pain level no higher than 3 on an 11-point Likert-type numeric verbal pain rating scale. Appropriate descriptive and analytic statistics were computed and significance was set at a P value less than .05. Of the 247 participants, 226 (91.5%) were women. The mean age of the sample was 38 ± 15.4 years. Successful surgical outcome was achieved in 62.3% of patients. Based on logistic regression analysis, higher initial mean pain score and concurrent use of benzodiazepines were the only variables that predicted an unsuccessful surgical outcome (P < .001; P = .005). Adverse effects were reported by 13.4% of patients, the most common being postoperative increase in pain (13.4%), temporary malocclusion (1.2%), and temporary paresthesia in the preauricular region (0.4%). The results from this study indicate that in patients with ID of the TMJ unresponsive to noninvasive treatments, high initial pain scores and concurrent use of benzodiazepines are correlated with an unsuccessful outcome after AS. Copyright © 2018. Published by

  19. Cell Lysis and Detoxification of Cyanotoxins Using a Novel Combination of Microbubble Generation and Plasma Microreactor Technology for Ozonation

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    Jagroop Pandhal

    2018-04-01

    Full Text Available There has been a steady rise in the incidences of algal blooms globally, and worryingly, there is increasing evidence that changes in the global climate are leading to a shift toward cyanobacterial blooms. Many cyanobacterial genera are harmful, producing several potent toxins, including microcystins, for which there are over 90 described analogues. There are a wide range of negative effects associated with these toxins including gastroenteritis, cytotoxicity, hepatotoxicity and neurotoxicity. Although a variety of oxidation based treatment methods have been described, ozonation and advanced oxidation are acknowledged as most effective as they readily oxidise microcystins to non-toxic degradation products. However, most ozonation technologies have challenges for scale up including high costs and sub-optimum efficiencies, hence, a low cost and scalable ozonation technology is needed. Here we designed a low temperature plasma dielectric barrier discharge (DBD reactor with an incorporated fluidic oscillator for microbubble delivery of ozone. Both technologies have the potential to drastically reduce the costs of ozonation at scale. Mass spectrometry analysis revealed very rapid (<2 min destruction of two pure microcystins (MC-LR and MC-RR, together with removal of by-products even at low flow rate 1 L min−1 where bubble size was 0.56–0.6 mm and the ozone concentration within the liquid was 20 ppm. Toxicity levels were calculated through protein phosphatase inhibition assays and indicated loss of toxicity as well as confirming the by-products were also non-toxic. Finally, treatment of whole Microcystis aeruginosa cells showed that even at these very low ozone levels, cells can be killed and toxins (MC-LR and Desmethyl MC-LR removed. Little change was observed in the first 20 min of treatment followed by rapid increase in extracellular toxins, indicating cell lysis, with most significant release at the higher 3 L min−1 flow rate compared to 1 L

  20. Influence of polymer architecture on antigens camouflage, CD47 protection and complement mediated lysis of surface grafted red blood cells.

    Science.gov (United States)

    Chapanian, Rafi; Constantinescu, Iren; Rossi, Nicholas A A; Medvedev, Nadia; Brooks, Donald E; Scott, Mark D; Kizhakkedathu, Jayachandran N

    2012-11-01

    Hyperbranched polyglycerol (HPG) and polyethylene glycol (PEG) polymers with similar hydrodynamic sizes in solution were grafted to red blood cells (RBCs) to investigate the impact of polymer architecture on the cell structure and function. The hydrodynamic sizes of polymers were calculated from the diffusion coefficients measured by pulsed field gradient NMR. The hydration of the HPG and PEG was determined by differential scanning calorimetry analyses. RBCs grafted with linear PEG had different properties compared to the compact HPG grafted RBCs. HPG grafted RBCs showed much higher electrophoretic mobility values than PEG grafted RBCs at similar grafting concentrations and hydrodynamic sizes indicating differences in the structure of the polymer exclusion layer on the cell surface. PEG grafting impacted the deformation properties of the membrane to a greater degree than HPG. The complement mediated lysis of the grafted RBCs was dependent on the type of polymer, grafting concentration and molecular size of grafted chains. At higher molecular weights and graft concentrations both HPG and PEG triggered complement activation. The magnitude of activation was higher with HPG possibly due to the presence of many hydroxyl groups per molecule. HPG grafted RBCs showed significantly higher levels of CD47 self-protein accessibility than PEG grafted RBCs at all grafting concentrations and molecular sizes. PEG grafted polymers provided, in general, a better shielding and protection to ABO and minor antigens from antibody recognition than HPG polymers, however, the compact HPGs provided greater protection of certain antigens on the RBC surface. Our data showed that HPG 20 kDa and HPG 60 kDa grafted RBCs exhibited properties that are more comparable to the native RBC than PEG 5 kDa and PEG 10 kDa grafted RBCs of comparable hydrodynamic sizes. The study shows that small compact polymers such as HPG 20 kDa have a greater potential in the generation of functional RBC for therapeutic

  1. ESTUDIOS (INTERCULTURALES EN CLAVE DE-COLONIAL

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    Catherine Walsh

    2010-01-01

    Full Text Available Los «estudios culturales» en América Latina forman parte de una política de nombrar inscrita en legados y cartografiados frecuentemente como totalidad, ocultando o dejando pasar por alto las diferencias a su interior. Este articula examina desde dónde nacen los estudios culturales en América Latina en general y en la Universidad Andina Simón Bolívar en Quito en particular, con qué política de nombramiento, qué proyecto(s y qué bases y perspectivas de conocimiento. Considera qué implica concebir y construir los estudios culturales como proyecto político-intelectual, inter-cultural, inter-epistémico y de orientación de-colonial y los desafíos y obstáculos al respecto, incluyendo dentro de la problemática misma de la «uni»-versidad.

  2. Proliferative and phenotypical characteristics of human adipose tissue-derived stem cells: comparison of Ficoll gradient centrifugation and red blood cell lysis buffer treatment purification methods.

    Science.gov (United States)

    Najar, Mehdi; Rodrigues, Robim M; Buyl, Karolien; Branson, Steven; Vanhaecke, Tamara; Lagneaux, Laurence; Rogiers, Vera; De Kock, Joery

    2014-09-01

    Adult human subcutaneous adipose tissue harbors a multipotent stem cell population, the so-called human adipose tissue-derived mesenchymal stromal cells (AT-MSCs). These cells are able to differentiate in vitro into various cell types and possess immunomodulatory features. Yet procedures to obtain AT-MSCs can vary significantly. The two most extensively used AT-MSC purification techniques are (i) density gradient centrifugation using Ficoll and (ii) red blood cell (RBC) lysis buffer treatment of the stromal vascular fraction. In the context of potential clinical cell therapy, the stem cell yield after purification and upon consecutive passages, as well as the purity of the obtained cell population, are of utmost importance. We investigated the expansion capacity and purity of AT-MSCs purified by both procedures immediately after isolation and upon consecutive passages. We also investigated possible purification-dependent differences in their expression of immune-inhibitory factors and cell adhesion molecules. We found that RBC lysis buffer treatment is a more robust and easier method to purify AT-MSCs than density gradient fractionation. However, the resulting AT-MSC-RBC population contains a significantly higher number of CD34(+) cells, particularly during the first passages after plating. From passage 4 onward, no significant differences could be observed between both populations with respect to the immunophenotype, expansion capacity and expression of immune inhibitory factors and cell adhesion molecules. Our data show that RBC lysis buffer treatment may be a good alternative to density fractionation, providing a faster, more robust and easier method to purify AT-MSCs with biologically preserved characteristics. Copyright © 2014 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

  3. An improved in-house lysis-filtration protocol for bacterial identification from positive blood culture bottles with high identification rates by MALDI-TOF MS.

    Science.gov (United States)

    Tsuchida, Sachio; Murata, Syota; Miyabe, Akiko; Satoh, Mamoru; Takiwaki, Masaki; Matsushita, Kazuyuki; Nomura, Fumio

    2018-05-01

    Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is now a well-established method for identification of microorganisms from positive blood cultures. Pretreatments to effectively remove non-bacterial proteins are a prerequisite for successful identification, and a variety of protocols have been reported. Although commercially available kits, mainly the Sepsityper Kit, are increasingly used, the identification rates reported often are not satisfactory, particularly for Gram-positive isolates. We developed a new, in-house lysis-filtration protocol and prospectively evaluated its performance compared to the Sepsityper kit. The in-house protocol consists of three simple steps: lysis by ammonium chloride, aspiration with a syringe fitted with a 0.45-μm membrane, and centrifugation to collect microbes. The novel protocol requires only 20 min. Performance of the in-house protocol was evaluated using a total of 117 monomicrobial cases of positive blood culture. Medium from blood culture bottles was pretreated by the in-house protocol or the commercial kit, and isolated cells were subjected to direct identification by mass spectrometry fingerprinting in parallel with conventional subculturing for reference identification. The overall MALDI-TOF MS-based identification rates with score > 1.7 and > 2.0 obtained using the in-house protocol were 99.2% and 85.5%, respectively, whereas those obtained using the Sepsityper Kit were 85.4% and 61.5%, respectively. For Gram-positive cases, the in-house protocol yielded scores >1.7 and > 2.0 at 98.5% and 76.1%, respectively, whereas the commercial kit yielded these scores at 76.1% and 43.3%, respectively. Although these are preliminary results, these values suggest that this easy lysis-filtration protocol deserves assessment in a larger-scale test. Copyright © 2018 Elsevier B.V. All rights reserved.

  4. Evaluation of cell lysis procedures and use of a micro fluidic system for an automated DNA-based cell identification in interplanetary missions

    Science.gov (United States)

    Hall, J. A.; Felnagle, E.; Fries, M.; Spearing, S.; Monaco, L.; Steele, A.

    2006-12-01

    A Modular Assay System for Solar System Exploration (MASSE) is being developed to include sample handling, pre-treatment, separation and analysis of biological target compounds by both DNA and protein microarrays. To better design sensitive and accurate initial upstream sample handling of the MASSE instrument, experiments investigating the sensitivity and potential extraction bias of commercially available DNA extraction kits between classes of environmentally relevant prokaryotes such as gram-negative bacteria ( Escherichia coli), gram-positive bacteria ( Bacillus megatarium), and Archaea ( Haloarcula marismortui) were performed. For extractions of both planktonic cultures and spiked Mars simulated regolith, FTA ® paper demonstrated the highest sensitivity, with detection as low as ˜1×10 1 cells and ˜3.3×10 2 cells, respectively. In addition to the highest sensitivity, custom modified application of FTA ® paper extraction protocol is the simplest in terms of incorporation into MASSE and displayed little bias in sensitivity with respect to prokaryotic cell type. The implementation of FTA paper for environmental microbiology investigations appears to be a viable and effective option potentially negating the need for other pre-concentration steps such as filtration and negating concerns regarding extraction efficiency of cells. In addition to investigations on useful technology for upstream sample handling in MASSE, we have also evaluated the potential for μTAS to be employed in the MASSE instrument by employing proprietary lab-on-a-chip development technology to investigate the potential for microfluidic cell lysis of different prokaryotic cells employing both chemical and biological lysis agents. Real-time bright-field microscopy and quantitative PMT detection indicated that that gram positive, gram negative and archaeal cells were effectively lyzed in a few seconds using the microfluidic chip protocol developed. This included employing a lysis buffer with

  5. Application of mitomycin C after endoscopic lysis of congenital laryngeal web combined with epiglottic hypoplasia in a middle-aged man.

    Science.gov (United States)

    Roh, Jong-Lyel

    2006-04-01

    Laryngeal webs and epiglottic hypoplasias are uncommon congenital anomalies. Anterior glottic web combined with epiglottic hypoplasia was found in a middle-aged man presenting with hoarseness and dyspnea on exertion. This can be considered as a unique isolated defect of the larynx during early fetal development. The laryngeal web can be successfully treated in a single stage with endoscopic lysis and topical application of mitomycin C for prevention of anterior glottic restenosis. This case and prior reports suggest that the novel approach may be effective in the treatment of laryngeal webs.

  6. Daratumumab-mediated lysis of primary multiple myeloma cells is enhanced in combination with the human anti-KIR antibody IPH2102 and lenalidomide

    DEFF Research Database (Denmark)

    Nijhof, I. S.; Lammerts van Bueren, J. J.; van Kessel, B.

    2015-01-01

    Despite recent treatment improvements, multiple myeloma remains an incurable disease. Since antibody-dependent cell-mediated cytotoxicity is an important effector mechanism of daratumumab, we explored the possibility of improving daratumumab-mediated cell-mediated cytotoxicity by blocking natural...... killer cell inhibitory receptors with the human monoclonal anti-KIR antibody IPH2102, next to activation of natural killer cells with the immune modulatory drug lenalidomide. In 4-hour antibody-dependent cell-mediated cytotoxicity assays, IPH2102 did not induce lysis of multiple myeloma cell lines...... effective treatment strategies can be designed for multiple myeloma by combining daratumumab with agents that independently modulate natural killer cell function....

  7. CTL lysis: there is a hyperbolic relation of killing rate to exocytosable granzyme A for highly cytotoxic murine cytotoxic T lymphocytes.

    Science.gov (United States)

    Poe, M; Wu, J K; Talento, A; Koo, G C

    1996-06-10

    The lysis of susceptible targets by efficient cytotoxic T lymphocytes (CTL) increases both with time and with the ratio of CTL to target. Simple methods for calculating a killing rate constant from the time dependence of killing and for calculating the relation of the killing rate constant to the concentration of exocytosable granzyme A are given. Application of these methods to the killing of target cells by the highly efficient mouse CTL AR1 is presented. AR1 needed granzyme A for efficient killing. AR1 contained sufficient exocytosable granzyme A to kill at about 80% of the rate possible at infinite exocytosable granzyme A.

  8. Changes in clot lysis levels of reteplase and streptokinase following continuous wave ultrasound exposure, at ultrasound intensities following attenuation from the skull bone

    Directory of Open Access Journals (Sweden)

    Roijer Anders

    2008-08-01

    Full Text Available Abstract Background Ultrasound (US has been used to enhance thrombolytic therapy in the treatment of stroke. Considerable attenuation of US intensity is however noted if US is applied over the temporal bone. The aim of this study was therefore to explore possible changes in the effect of thrombolytic drugs during low-intensity, high-frequency continuous-wave ultrasound (CW-US exposure. Methods Clots were made from fresh venous blood drawn from healthy volunteers. Each clot was made from 1.4 ml blood and left to coagulate for 1 hour in a plastic test-tube. The thrombolytic drugs used were, 3600 IU streptokinase (SK or 0.25 U reteplase (r-PA, which were mixed in 160 ml 0.9% NaCl solution. Continuous-wave US exposure was applied at a frequency of 1 MHz and intensities ranging from 0.0125 to 1.2 W/cm2. For each thrombolytic drug (n = 2, SK and r-PA and each intensity (n = 9 interventional clots (US-exposed, n = 6 were submerged in thrombolytic solution and exposed to CW-US while control clots (also submerged in thrombolytic solution, n = 6 were left unexposed to US. To evaluate the effect on clot lysis, the haemoglobin (Hb released from each clot was measured every 20 min for 1 hour (20, 40 and 60 min. The Hb content (mg released was estimated by spectrophotometry at 540 nm. The difference in effect on clot lysis was expressed as the difference in the amount of Hb released between pairs of US-exposed clots and control clots. Statistical analysis was performed using Wilcoxon's signed rank test. Results Continuous-wave ultrasound significantly decreased the effects of SK at intensities of 0.9 and 1.2 W/cm2 at all times (P 2 and at 1.2 W/cm2, following 40 min exposure at 0.3, 0.6, 0.9 and at 1.2 W/cm2, and following 60 min of exposure at 0.05 0.3, 0.6, 0.9 and at 1.2 W/cm2 (all P Conclusion Increasing intensities of CW-US exposure resulted in increased clot lysis of r-PA-treated blood clots, but decreased clot lysis of SK-treated clots.

  9. Transcatheter arterial embolization with trisacryl gelatin microspheres (Embosphere®) leads to life-threatening tumor lysis syndrome in a rectal carcinoid patient with hepatic metastases

    International Nuclear Information System (INIS)

    Lo, Yuan-Hao; Tsai, Ming-Tsun; Kuo, Chen-Yu; Liu, Wen-Sheng; Lee, Rheun-Chuan; Yeh, Yi-Chen; Li, Chung-Pin; Chen, Jinn-Yang; Chao, Yee

    2012-01-01

    The incidence of gastrointestinal carcinoids appears to be increasing, and the rectum is the third most common location. Transcatheter arterial embolization (TAE) with trisacryl gelatin microspheres (Embosphere ® ) has been reported as an effective method for hepatic metastases of rectal carcinoids. Complications are uncommon and usually of minor consequence. We report an unusual case of a 34-year-old man with tumor lysis syndrome following TAE with Embosphere ® in a patient with multiple hepatic metastases of a rectal carcinoid. Early detection and effective treatment are essential for this rare but potentially catastrophic complication

  10. Análise da produção do conhecimento em processo de enfermagem: estudo exploratório-descritivo Análisis de la producción de conocimiento sobre el proceso de enfermería: estudio exploratorio-descriptivo Analysis of knowledge production on the nursing process: exploratory-descriptive study

    Directory of Open Access Journals (Sweden)

    Erika Christiane Marocco Duran

    2011-06-01

    Full Text Available Estudo descritivo-exploratório que objetiva analisar a produção do conhecimento em processo de enfermagem com base nas dissertações de mestrado e teses de doutorado defendidas nos programas de pós-graduação em enfermagem do Brasil, por meio dos relatórios do Centro de Estudos e Pesquisas em Enfermagem (CEPEn de 1972 a 2007 e diagnosticar as publicações em bases de dados indexadas. Encontrou-se 122 dissertações de mestrado, com 42 publicações, 26 teses de doutorado, com 15 publicações. Verificou-se maior publicação a partir de 2000, sendo a pesquisa qualitativa mais prevalente. A tendência temática mais evidenciada foi assistência de enfermagem, sendo o levantamento e validação de diagnóstico de enfermagem e todas as fases do processo os assuntos mais abordados. Existem lacunas nas publicações sobre o tema, sobretudo no levantamento da produção do conhecimento. Pesquisas que analisem esta interface possivelmente podem qualificar a implementação do processo de enfermagem.Estudio descriptivo-exploratorio con objeto de analizar la producción de conocimiento sobre el proceso de enfermería basado en las disertaciones de maestría y tesis de doctorado defendidas en los programas de postgrado en enfermería de Brasil, mediante los informes del Centro de Estudios e Investigaciones en Enfermería (CEPEn entre 1972 y 2007 y diagnosticar las publicaciones en bases de datos indexadas. Fueron encontradas 122 disertaciones de maestría, con 42 publicaciones, y 26 tesis de doctorado, con 15 publicaciones. Se verificó mayor publicación a partir de 2000, siendo la investigación cualitativa más prevalente. La tendencia temática más evidenciada fue el asistencia de enfermería, siendo el análisis y la validación de diagnóstico de enfermería y todas las fases del proceso los tópicos más tratados. Existen lagunas en las publicaciones sobre el tema, sobretodo en el análisis de la producción de conocimiento

  11. Lisis de adherencias epidurales (Parte 1): Fisiopatología y clínica Percutaneous Epidural lysis of adhesions

    OpenAIRE

    G. Racz; J. C. Flores

    2012-01-01

    Según estudios epidemiológicos toda la población tiene altas probabilidades de sufrir dolor lumbar en algún momento de su vida. Este cuadro suele ser reconocido como lumbalgia o lumbociática. En su forma clínica más común se observa mejoría rápida y solo presentan síntomas persistentes 5-10% de los casos (1). En la década de 1990 en EEUU, el sector salud registró costes estimados en miles de millones de dólares destinados al tratamiento del dolor lumbar, y teniendo en cuenta la tendencia a la...

  12. Identidad y producción del espacio en los procesos de transformación y especialización de la ciudad. Estudio de caso del barrio Justicia (Chueca / Identity and space production in the processes of transformation and specialization of the city.

    Directory of Open Access Journals (Sweden)

    Helga von Breymann Miranda

    2011-09-01

    Full Text Available ResumenLa reflexión teórica más reciente se ha interesado por la relación entre las transformaciones urbanas asociadas a los movimientos sociales, los procesos de formación identitaria y las políticas urbanas. En esta investigación se realiza un breve repaso por aportes teóricos relevantes desde un plano general –la importancia del vínculo del planeamiento con los procesos de diferenciación social– a otro particular –el desarrollo de una espacialidad LGBT (Lesbiana, Gay, Bisexual y Transexual en sectores concretos de la ciudad¬. A continuación, el artículo expone como caso de estudio, el proceso de transformación que ha llevado el barrio de Justicia/Chueca, hoy reconocido barrio gay de Madrid, en las dos últimas décadas. Los resultados obtenidos, al confrontar la teoría con el caso del barrio madrileño, permitieron valorar la importancia de la identidad del colectivo LGBT y los vecinos del barrio en el proceso de cambio urbano de Justicia.Palabras clave Transformación urbana, movimientos sociales, Chueca, barrio gay, LGBT. AbstractMost recent theoretic thought has gained an interest on the relation between urban transformation as linked to social movement, identity processes and urban politics. This investigation briefly summarizes relevant theoretical support from general aspects such as the importance of linking planning with processes of social differentiation. It also summarizes the mentioned support to a particular aspect, which is the development of a LGBT (Lesbian, Gay, Bisexual and Transexual spatiality in specific areas of the city. Further, the article refers as case study to the transformation process that the Justicia/Chueca neighborhood, today known as the gay neighborhood of Madrid, has undergone in the last two decades. The results obtained by counter posing theory with Madrid’s neighborhood has allowed placement of value to the importance of the identity of the LGBT community and its neighbors in the

  13. ESTUDIOS TRANSCULTURALES DEL BURNOUT: LOS ESTUDIOS TRANSCULTURALES BRASIL-ESPAÑA

    Directory of Open Access Journals (Sweden)

    Macarena Gálvez Herrer

    2003-07-01

    Full Text Available Los estudios transculturales no son un ejercicio secundario y complementario en el estudio de la psicología; un modelo antropológico de la conducta humana obliga a plantear que las variables culturales no son secundarias sino primarias. Por estas razones el estudio del “burnout†o desgaste profesional puede profundizarse mediante la utilización de los métodos transculturales. No es suficiente con la simple comparación de resultados, sino que es necesario buscar instrumentos propios de cada cultura y establecer la comparación no sólo del síndrome de desgaste profesional sino de todos los diferentes elementos del proceso. El trabajo presentado establece las bases teóricas de este planteamiento, y expone, mediante un ejemplo, algunas de las vías posibles para una psicología transcultural del burnout.

  14. Estudios transculturales del burnout: Los estudios transculturales Brasil-España

    Directory of Open Access Journals (Sweden)

    Bernardo Moreno Jiménez

    2003-01-01

    Full Text Available Los estudios transculturales no son un ejercicio secundario y complementario en el estudio de la psicología; un modelo antropológico de la conducta humana obliga a plantear que las variables culturales no son secundarias sino primarias. Por estas razones el estudio del “burnout” o desgaste profesional puede profundizarse mediante la utilización de los métodos transculturales. No es suficiente con la simple comparación de resultados, sino que es necesario buscar instrumentos propios de cada cultura y establecer la comparación no sólo del síndrome de desgaste profesional sino de todos los diferentes elementos del proceso. El trabajo presentado establece las bases teóricas de este planteamiento, y expone, mediante un ejemplo, algunas de las vías posibles para una psicología transcultural del burnout

  15. Fibrin-specific and effective clot lysis requires both plasminogen activators and for them to be in a sequential rather than simultaneous combination.

    Science.gov (United States)

    Pannell, R; Li, S; Gurewich, V

    2017-08-01

    Thrombolysis with tissue plasminogen activator (tPA) has been a disappointment and has now been replaced by an endovascular procedure whenever possible. Nevertheless, thrombolysis remains the only means by which circulation in a thrombosed artery can be restored rapidly. In contrast to tPA monotherapy, endogenous fibrinolysis uses both tPA and urokinase plasminogen activator (uPA), whose native form is a proenzyme, prouPA. This combination is remarkably effective as evidenced by the fibrin degradation product, D-dimer, which is invariably present in plasma. The two activators have complementary mechanisms of plasminogen activation and are synergistic in combination. Since tPA initiates fibrinolysis when released from the vessel wall and prouPA is in the blood, they induce fibrinolysis sequentially. It was postulated that this may be more effective and fibrin-specific. The hypothesis was tested in a model of clot lysis in plasma in which a clot was first exposed to tPA for 5 min, washed and incubated with prouPA. Lysis was compared with that of clots incubated with both activators simultaneously. The sequential combination was almost twice as effective and caused less fibrinogenolysis than the simultaneous combination (p < 0.0001) despite having significantly less tPA, as a result of the wash. A mechanism is described by which this phenomenon can be explained. The findings are believed to have significant therapeutic implications.

  16. A Simple Method for DNA Extraction from Mature Date Palm Leaves: Impact of Sand Grinding and Composition of Lysis Buffer

    Directory of Open Access Journals (Sweden)

    Mohammad Al Sadoon

    2010-09-01

    Full Text Available Molecular marker techniques have been widely used for cultivar identification of inbred date palms (Phoenix dactylifera L.; Arecaceae and biodiversity conservation. Isolation of highly pure DNA is the prerequisite for PCR amplification and subsequent use such as DNA fingerprinting and sequencing of genes that have recently been developed for barcoding. To avoid problems related to the preservation and use of liquid nitrogen, we examined sterile sand for grinding the date palm leaves. Individual and combined effects of sodium chloride (NaCl, polyvinylpyrrolidone (PVP and lithium chloride (LiCl with the cetyltrimethylammonium bromide (CTAB method for a DNA yield of sufficient purity and PCR amplification were evaluated in this study. Presence of LiCl and PVP alone or together in the lysis buffer did not significantly improve the DNA yield and purity compared with the addition of NaCl. Our study suggested that grinding of date palm leaf with sterile sand and inclusion of NaCl (1.4 M in the lysis buffer without the costly use of liquid nitrogen, PVP and LiCl, provides a DNA yield of sufficient purity, suitable for PCR amplification.

  17. TNF induction of EL4 hyposensitivity to lysis by recombinant (soluble) and membrane-associated TNFs: TNF binding, internalization, and degradation.

    Science.gov (United States)

    Fishman, M; Costlow, M

    1994-04-01

    EL4 mouse thymoma cells sensitive to TNF-mediated lysis only in the presence of cycloheximide (S-EL4) or in the presence or absence of cycloheximide (N-EL4) were used in these experiments. Murine tumor cell line (S-EL4) sensitivity to TNF cytotoxicity is augmented when cycloheximide is added together with TNF or when cycloheximide is added 1 hr before or after TNF. No enhanced sensitivity is observed when target cells are incubated with cycloheximide 2-4 hr before or after the addition of TNF. In the absence of cycloheximide, S-EL4 cells preexposed to murine TNF are less susceptible to lysis by TNF and TNF receptor-conjugated TNF but are lysed by integral membrane TNF. TNF-induced hyposensitivity is partially reversed by actinomycin D or by culturing the preexposed cells for 4 hr prior to TNF lytic assay. TNF preincubation of N- and S-EL4 cells results in an immediate decrease in 125I-TNF binding due to TNF receptor occupancy. Recovery of TNF-R occupancy and TNF internalization were subsequently noted.

  18. ESTUDIO DE FRACCIONES EN CONTEXTOS SONOROS

    Directory of Open Access Journals (Sweden)

    Alexander Conde

    2016-01-01

    Full Text Available En este ensayo resaltamos los vínculos cognitivos entre las matemáticas y la música, que pueden favorecer procesos de enseñanza y aprendizaje de las fracciones en la matemática escolar. Las actividades exhibidas provienen de una investigación que promueve experiencias sensoriales en el campo rítmico para favorecer la construcción de nociones matemáticas. Dichas actividades posibilitan un engranaje armónico entre las matemáticas y la música convergentes en el tiempo y el sonido como los objetos de estudio común entre éstas disciplinas. El análisis de la investigación que aquí reportamos es producto de la aplicación de las actividades en diferentes programas de formación de profesorado de México y Francia, alrededor de la enseñanza y aprendizaje de las matemáticas en contextos interdisciplinarios. Este análisis se desarrolló desde tres categorías referentes a las nociones de unidad relativa, relación parte-parte y equipartición, las cuales son nociones fundamentales para el estudio de las fracciones en la matemática escolar. Encontramos que la enseñanza con enfoque integrador requiere no solo conocimientos especializados, sino un cambio de creencias en torno a las opiniones del profesorado sobre la organización del currículo, su enseñanza y la manera en que aprende la población estudiantil. Un aporte de esta experiencia es ofrecer a docentes elementos teóricos y didácticos para el estudio de las fracciones en contextos interdisciplinarios.

  19. Estudio de un caso de control interno

    OpenAIRE

    Alfonso Pirela

    2005-01-01

    El estudio se efectuó con el objetivo de analizar el control interno en el Almacén de la Facultad de ciencias Económicas y Sociales de la Universidad del Zulia. La metodología fue descriptiva. Se utilizó como población a todo el personal del almacén. Los resultados arrojaron que el control del almacén no cuenta con un sistema de control interno integrado que le permita llevar con efectividad las actividades de recepción, almacenamiento y despacho de la mercancía.

  20. Estudio de un caso de control interno

    Directory of Open Access Journals (Sweden)

    Alfonso Pirela

    2005-09-01

    Full Text Available El estudio se efectuó con el objetivo de analizar el control interno en el Almacén de la Facultad de ciencias Económicas y Sociales de la Universidad del Zulia. La metodología fue descriptiva. Se utilizó como población a todo el personal del almacén. Los resultados arrojaron que el control del almacén no cuenta con un sistema de control interno integrado que le permita llevar con efectividad las actividades de recepción, almacenamiento y despacho de la mercancía.

  1. Estudio sobre redes sociales y estudiantes

    OpenAIRE

    Toledo Morales, Purificación; Sánchez García, José Manuel

    2012-01-01

    En este estudio pretendemos analizar cómo una muestra de 150 estudiantes utiliza las redes sociales, y explorar el impacto de estas en el rendimiento académico de los mismos. Los datos recogidos de la aplicación y análisis de una escala de opinión revelaron que mas de la mitad de los estudiantes encuestados utilizan internet con una finalidad puramente recreativa, y las redes sociales para estar en contacto con los amigos. La mayoría de los encuestados rechazan las solicitudes de amistad de d...

  2. Amplificadores con transistores. Estudio y dimensionado

    OpenAIRE

    Lubiano García, Adrián

    2017-01-01

    Este trabajo es un estudio de las distintas configuraciones de los amplificadores con transistores vistos en la asignatura de Electrónica Analógica del tercer curso del Grado en Ingeniería en Electrónica Industrial y Automática de la Escuela de Ingenierías Industriales de la Universidad de Valladolid. En este trabajo se mostrarán los pasos seguidos en la creación de una aplicación con Visual Basic para la realización de los ejercicios de las distintas configuraciones, así...

  3. Estudio del álbum sin palabras

    OpenAIRE

    Bosch Andreu, Emma

    2015-01-01

    [spa] Estudio del Álbum Sin Palabras tiene como objetivo principal definir, analizar y categorizar los álbumes sin palabras destinados principalmente al público infantil y juvenil dando a conocer sus características, peculiariades y diversidad tipológica, para facilitar las tareas de análisis y de mediación de investigadores, educadores, bibliotecarios y cualquier persona interesada en los libros con imágenes. En el capítulo titulado «Un nuevo mapa para los Libros Sin Palabras» situamos e...

  4. Anorexia nervosa: un estudio de casos

    OpenAIRE

    Zusman, Lillyana

    2013-01-01

    La Anorexia Nervosa es un trastorno de alimentación que se define (etimológicamente) como una "pérdida nerviosa del apetito". Se caracteriza por la actitud consciente, voluntaria y rotunda de los sujetos  de tener un exceso de peso que intentan modificar por vía de la inanición. A partir del estudio de casos, se propone la distinción entre una Anorexia Nervosa Estructural -aquella en la que predomina el conflicto intrapsíquico primario y arcaico, y que manifiesta una conducta aislada y retraí...

  5. Estudio sobre encofrados de madera modernos

    Directory of Open Access Journals (Sweden)

    de la Peña Aznar, Juan M.

    1980-11-01

    Full Text Available The author continues the development of the subject «Modern Timber Formwork», by summing up the comparative examination —already carried out in Part III of this Study— of the different types of glued timber existing on the market. In addition, the loads and stresses allowable for coniferous timber and the proposal for establishing Regulations for the Timber Section of the Research and Experimental Forestry Institute of Spain are given. In part V of the author's Study, published in this article, the important subject of the glues used for joining timber, a truly vital point in order to obtain louvered timber beams which are simply glued together, is approached.

    El autor continúa el desarrollo del tema sobre «Encofrados de madera modernos», resumiendo el estudio comparativo —ya hecho en la Parte III de este Estudio— de las diferentes vigas de madera encolada existentes en el mercado dando, además, las cargas y tensiones admisibles para maderas coníferas y la propuesta de Reglamentación de la Sección de Maderas del Instituto Forestal de Investigaciones y Experiencias de España. En la parte V del Estudio del autor, publicada en este articulo, se aborda el importante tema de las colas empleadas para las uniones de madera, algo realmente vital para la obtención de vigas de madera en celosía simplemente encoladas.

  6. Diphtheria toxin- and Pseudomonas A toxin-mediated apoptosis. ADP ribosylation of elongation factor-2 is required for DNA fragmentation and cell lysis and synergy with tumor necrosis factor-alpha.

    Science.gov (United States)

    Morimoto, H; Bonavida, B

    1992-09-15

    We have reported that diphtheria toxin (DTX) mediates target cell lysis and intranucleosomal DNA fragmentation (apoptosis) and also synergizes with TNF-alpha. In this paper, we examined which step in the pathway of DTX-mediated inhibition of protein synthesis was important for induction of cytolytic activity and for synergy. Using a DTX-sensitive tumor cell line, we first examined the activity of the mutant CRM 197, which does not catalyze the ADP ribosylation of elongation factor-2 (EF-2). CRM 197 was not cytolytic for target cells and did not mediate intranucleosomal DNA fragmentation of viable cells. The failure of CRM 197 to mediate target cell lysis suggested that the catalytic activity of DTX is prerequisite for target cell lysis. This was corroborated by demonstrating that MeSAdo, which blocks the biosynthesis of diphthamide, inhibited DTX-mediated protein synthesis inhibition and also blocked target cell lysis. Furthermore, the addition of nicotinamide, which competes with NAD+ on the DTX action site of EF-2, also blocked DTX-mediated lysis. These findings suggest that ADP-ribosylation of EF-2 may be a necessary step in the pathway leading to target cell lysis. In contrast to the sensitive line, the SKOV-3 tumor cell line is sensitive to protein synthesis inhibition by DTX but is not susceptible to cytolysis and apoptosis by DTX. Thus, protein synthesis inhibition by DTX is not sufficient to mediate target cell lysis. The synergy in cytotoxicity obtained with the combination of DTX and TNF-alpha was examined in order to determine the pathway mediated by DTX in synergy. Like the direct lysis by DTX, synergy was significantly reduced by MeSAdo and by nicotinamide. Furthermore, synergy was not observed with combination of CRM 197 and TNF-alpha. These results demonstrate that, in synergy, DTX may utilize the same pathway required for its cytolytic activity. Pseudomonas aeruginosa exotoxin shared most the properties shown for DTX. Altogether, these findings

  7. Estudios Experimentales 1 Parte: El Ensayo Clínico

    OpenAIRE

    Manterola, Carlos; Otzen, Tamara

    2015-01-01

    Los estudios experimentales, se caracterizan por la valoración del efecto de una o más intervenciones, habitualmente de forma comparativa con otra intervención, o un placebo; y el carácter prospectivo, de la recolección de los datos y el seguimiento de los grupos en estudio. Bajo la denominación de estudios experimentales, existe una diversidad de diseños, desde los ensayos clínicos (EC) y sus variantes, hasta los estudios cuasi-experimentales y los experientos naturales. El objetivo de este ...

  8. Estudio “Cumple con SAM”

    Directory of Open Access Journals (Sweden)

    Escribá MP

    2009-06-01

    Full Text Available INTRODUCCIÓN Existen estudios que demuestran los beneficios de los sistemas personalizados de dosificación para aumentar la adherencia al tratamiento en pacientes polimedicados. Recientemente se puso en el mercado un sistema de administración de medicamentos (SAM, portable y de pequeño tamaño, que combina un reloj con alarmas programables y varios compartimentos en donde colocar las formas farmacéuticas de los medicamentos. Para la valoración de su utilidad se ha realizado un estudio piloto de satisfacción sobre el SAM entre los pacientes diana de estos dispositivos, solicitando, tras un breve período de uso, su opinión sobre las características del mismo. Se ha evaluando también la mejora del cumplimiento. MATERIAL Y MÉTODOS 43 farmacéuticos comunitarios voluntarios de 26 oficinas de farmacia ubicadas en 5 provincias reclutaron sobre la base de unos criterios de inclusión y exclusión 302 pacientes incumplidores para que utilizasen durante al menos 15 días el SAM y, posteriormente, rellenasen una encuesta de opinión sobre éste. RESULTADOS La opinión del 91% de los pacientes participantes en el estudio es que el SAM es útil, muy útil o imprescindible como sistema de administración de medicamentos. El incumplimiento autocomunicado pasa del 87% al 23% de pacientes. El SAM puede ser útil para los pacientes como sistema de administración de medicamentos en la mejora de la adherencia a su tratamiento farmacológico en la medida en que disminuye los olvidos. Sin embargo, también ponen de manifiesto algunos problemas de diseño mejorables como son el excesivo tamaño, dificultad de apertura e intensidad del sonido. Estos dos últimos problemas dificultan su uso entre la población de mayor edad.

  9. Un estudio sobre el rendimiento universitario

    Directory of Open Access Journals (Sweden)

    Albaladejo Pina, I. P.

    1999-01-01

    Full Text Available El objetivo del presente trabajo es contrastar los rendimientos académicos de alumnos de dos cursos de la Facultad de Ciencias Económicas y Empresariales de la Universidad de Murcia, uno con el antiguo plan de estudios y otro con el actualmente en vigor. Analizamos el éxito y el fracaso para los estudiantes de cada uno de estos cursos, así como las variables socioeconómicas y educacionales que pueden influir en su rendimiento. Comprobamos, mediante unas estimaciones logit, que tanto unas como otras variables son factores condicionantes de este rendimiento. En particular, destacamos la influencia de haber cursado la opción C de COU en cuanto a fracasar o no en su primer curso universitario.

  10. Estudio sobre encofrados de madera modernos

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    de la Peña Aznar, Juan M.

    1980-12-01

    Full Text Available The author continues the development of the subject «Modern Timber Formwork», by summing up the comparative examination —already carried out in Part III of this Study— of the different types of glued timber existing on the market. In addition, the loads and stresses allovk/able for coniferous timber and the proposal for establishing Regulations for the Timber Section of the Research and Experimental Forestry Institute of Spain are given. In part V of the author's Study, published in this article, the important subject of the glues used for joining timber, a truly vital point in order to obtain louvered timber beams which are simply glued together, is approached.

    El autor continúa el desarrollo del tema sobre «Encofrados de madera modernos», resumiendo el estudio comparativo —ya hecho en la Parte III de este Estudio— de las diferentes vigas de madera encolada existentes en el mercado dando, además, las cargas y tensiones admisibles para maderas coníferas y la propuesta de Reglamentación de la Sección de Maderas del Instituto Forestal de Investigaciones y Experiencias de España. En la parte V del Estudio del autor, publicada en este artículo, se aborda el importante tema de las colas empleadas para las uniones de madera, algo realmente vital para la obtención de vigas de madera en celosía simplemente encoladas.

  11. Lysis to Kill: Evaluation of the Lytic Abilities, and Genomics of Nine Bacteriophages Infective for Gordonia spp. and Their Potential Use in Activated Sludge Foam Biocontrol.

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    Zoe A Dyson

    Full Text Available Nine bacteriophages (phages infective for members of the genus Gordonia were isolated from wastewater and other natural water environments using standard enrichment techniques. The majority were broad host range phages targeting more than one Gordonia species. When their genomes were sequenced, they all emerged as double stranded DNA Siphoviridae phages, ranging from 17,562 to 103,424 bp in size, and containing between 27 and 127 genes, many of which were detailed for the first time. Many of these phage genomes diverged from the expected modular genome architecture of other characterized Siphoviridae phages and contained unusual lysis gene arrangements. Whole genome sequencing also revealed that infection with lytic phages does not appear to prevent spontaneous prophage induction in Gordonia malaquae lysogen strain BEN700. TEM sample preparation techniques were developed to view both attachment and replication stages of phage infection.

  12. In black South Africans from rural and urban communities, the 4G/5G PAI-1 polymorphism influences PAI-1 activity, but not plasma clot lysis time.

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    Zelda de Lange

    Full Text Available Data on genetic and environmental factors influencing PAI-1 levels and their consequent effect on clot lysis in black African populations are limited. We identified polymorphisms in the promoter area of the PAI-1 gene and determined their influence on PAI-1act levels and plasma clot lysis time (CLT. We also describe gene-environment interactions and the effect of urbanisation. Data from 2010 apparently healthy urban and rural black participants from the South African arm of the PURE study were cross-sectionally analysed. The 5G allele frequency of the 4G/5G polymorphism was 0.85. PAI-1act increased across genotypes in the urban subgroup (p = 0.009 but not significantly in the rural subgroup, while CLT did not differ across genotypes. Significant interaction terms were found between the 4G/5G polymorphism and BMI, waist circumference and triglycerides in determining PAI-1act, and between the 4G/5G polymorphism and fibrinogen and fibrinogen gamma prime in determining CLT. The C428T and G429A polymorphisms did not show direct relationships with PAI-1act or CLT but they did influence the association of other environmental factors with PAI-1act and CLT. Several of these interactions differed significantly between rural and urban subgroups, particularly in individuals harbouring the mutant alleles. In conclusion, although the 4G/5G polymorphism significantly affected PAI-1act, it contributed less than 1% to the PAI-1act variance. (Central obesity was the biggest contributor to PAI-1act variance (12.5%. Urbanisation significantly influenced the effect of the 4G/5G polymorphism on PAI-1act as well as gene-environment interactions for the C428T and G429A genotypes in determining PAI-1act and CLT.

  13. In black South Africans from rural and urban communities, the 4G/5G PAI-1 polymorphism influences PAI-1 activity, but not plasma clot lysis time.

    Science.gov (United States)

    de Lange, Zelda; Rijken, Dingeman C; Hoekstra, Tiny; Conradie, Karin R; Jerling, Johann C; Pieters, Marlien

    2013-01-01

    Data on genetic and environmental factors influencing PAI-1 levels and their consequent effect on clot lysis in black African populations are limited. We identified polymorphisms in the promoter area of the PAI-1 gene and determined their influence on PAI-1act levels and plasma clot lysis time (CLT). We also describe gene-environment interactions and the effect of urbanisation. Data from 2010 apparently healthy urban and rural black participants from the South African arm of the PURE study were cross-sectionally analysed. The 5G allele frequency of the 4G/5G polymorphism was 0.85. PAI-1act increased across genotypes in the urban subgroup (p = 0.009) but not significantly in the rural subgroup, while CLT did not differ across genotypes. Significant interaction terms were found between the 4G/5G polymorphism and BMI, waist circumference and triglycerides in determining PAI-1act, and between the 4G/5G polymorphism and fibrinogen and fibrinogen gamma prime in determining CLT. The C428T and G429A polymorphisms did not show direct relationships with PAI-1act or CLT but they did influence the association of other environmental factors with PAI-1act and CLT. Several of these interactions differed significantly between rural and urban subgroups, particularly in individuals harbouring the mutant alleles. In conclusion, although the 4G/5G polymorphism significantly affected PAI-1act, it contributed less than 1% to the PAI-1act variance. (Central) obesity was the biggest contributor to PAI-1act variance (12.5%). Urbanisation significantly influenced the effect of the 4G/5G polymorphism on PAI-1act as well as gene-environment interactions for the C428T and G429A genotypes in determining PAI-1act and CLT.

  14. La auditoria sociolaboral y los estudios de relaciones laborales

    OpenAIRE

    Benito Bermejo, José Luis

    2016-01-01

    Se analizan los estudios de Relaciones Laborales en relación al perfil profesional de auditor sociolaboral, haciendo un repaso de la historia de ambos, así como comparando la titulación con otras titulaciones. Se concluye con una propuesta de futuro para los estudios de Relaciones Laborales. Grado en Relaciones Laborales y Recursos Humanos (Segovia)

  15. Estudios sobre postmodernidad y estudios culturales: ¿sinónimos?

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    Dr. Roberto A. Follari

    2000-01-01

    Full Text Available La postmodernidad exige para ser comprendida una dimensión sociohistórica y otra filosófica que le resulta irrenunciables. También hay una serie de fenómenos propiamente culturales, que son los que los denominados "estudios culturales" (G. Canclini. M. Barbero, etc. han asumido en Latinoamérica. Tales estudios han aportado una descripción de fenómenos nuevos, que no son analizados por ningún otro discurso teórico (tribus urbanas, identidades lábiles, influencia de los medios en los nuevos estilos de consumo, etc.. Con ello se da cuenta de parte de la cuestión postmodernidad, pero sin dudase deja fuera otras: lo filosófico y aún lo social y lo político quedan fuera de esa especie de antropología del presente. Por lo tanto, un enfoque integral de lo postmoderno incluye los estudios culturales, pero está lejos de limitarse a ellos.

  16. Escala de Metas de Estudio para Estudiantes Universitarios

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    María Victoria Pérez Villalobos

    2009-01-01

    Full Text Available Actualmente disponemos de gran cantidad de trabajos que muestran la implicación de las metas de estudio en la motivación por estudiar y aprender. Este trabajo analiza la motivación académica y, especialmente, la variable "metas de estudio". El principal propósito de esta investigación es conocer las características psicométricas de la adaptación de la Escala de Metas de Estudio a la población universitaria chilena. Los participantes son 542 estudiantes chilenos, de distintas facultades universitarias. Los resultados del análisis factorial y de consistencia interna son aceptables en las tres subescalas. Estos resultados fundamentan el uso de la Escala de Metas de Estudio para evaluar la motivación al estudio de alumnas y alumnos universitarios en Chile (CL.

  17. PARATIROIDECTOMIA ENDOSCOPICA. ESTUDIO EXPERIMENTAL EN EL PERRO

    Directory of Open Access Journals (Sweden)

    Jorge Ramón Lucena Olavarrieta

    2008-01-01

    Full Text Available La primera paratiroidectomía exitosa fue realizada en Viena, Austria, en 1925. Los procedimientos laparoscópicos estuvieron inicialmente limitadas a las zonas corporales con cavidades preexistentes, recientemente, el acceso a espacios anatómicos potenciales ha extendido las indicaciones de la cirugía endoscópica minimamnete invasiva.Desde el primer reporte sobre la paratiroidectomía endoscópica en 1996, las técnicas video-asistidas han comenzado a utilizarse en la cirugía del cuello, y en varias series se han documentado la factibilidad y seguridad de este tipo de abordaje. La finalidad de esta investigación fue el determinar la factibilidad, seguridad y eficacia de la paratiroidectomía endoscopia en el modelo canino.Entre el 25 de noviembre del 2005 y 15 de octubre del 2007, se intervinieron en el Instituto de Cirugía Experimental de la Universidad Central de Venezuela 31 perros, mestizos sanos desparasitados,asignándose a dos grupos: homogéneos "A" 16, a cirugía convencional, "B" 15 a cirugía video-asistida. Se midieron las siguientes variables; sexo, peso, exámenes de laboratorio, laringoscopia pre y postoperatoria, posición, dirección de la incisión, longitud, localización, valores pre y postoperatorios de calcio, fósforo, proteína C reactiva, hemoglobina, hematocrito, tipo de disección, número y posición de las paratiroides, identificación de los nervios recurrentes y rama externa del nervio laríngeo superior, sangramiento, dificultades intraoperatorias, tiempo, resultado del estudio histopatológico.Las deducciones de este estudio experimental proporcionan evidencias de que la paratiroidectomía endoscópica es factible y segura. El abordaje mínimo tiene mejores resultados cosméticos y el potencial de disminuir la morbi-mortalidad.

  18. Estudio de estabilidad de tabletas de propiltiouracilo 50 mg

    Directory of Open Access Journals (Sweden)

    María Olga Valdés Bendoyro

    Full Text Available Se desarrolló el estudio de estabilidad de las tabletas de propiltiouracilo 50 mg y se determinó su fecha de vencimiento. Este estudio se realizó por los métodos de vida de estante y de estabilidad acelerada mediante cromatografía líquida de alta eficiencia, validados en el Centro de Investigación y Desarrollo de Medicamentos. El estudio de vida de estante se desarrolló por un periodo de 24 meses a temperatura ambiente; mientras que el estudio de estabilidad acelerada se efectuó sometiendo el producto a la influencia de la luz, la humedad y la temperatura; se realizó el análisis durante 3 meses, para los 2 primeros y durante 6 meses para el estudio de la temperatura. La formulación de propiltiouracilo tabletas 50 mg cumplió con las especificaciones de calidad descritas en la farmacopea. Los resultados del estudio de estabilidad por vida de estante después de transcurridos los 24 meses indicaron que el producto mantenía los parámetros que determinan su calidad durante ese tiempo, y en los estudios acelerados no se observó degradación significativa del producto. Se estableció 2 años como fecha de vencimiento en las condiciones señaladas.

  19. planes de estudio y orientaciones oficiales

    Directory of Open Access Journals (Sweden)

    María Eugenia Fernández Fraile

    2005-01-01

    Full Text Available El presente artículo se propone, a través del análisis de los cambios introducidos en los Planes de estudios que más incidencia tienen sobre la enseñanza de las lenguas vivas (1900, 1926, 1934, 1938, 1953/1957, 1970 y 1990, destacar las tendencias evolutivas o las líneas maestras que configuran el concepto del Francés como Lengua Extranjera en tanto que disciplina escolar Se abordan así, en una presentación cronológica, las siguientes cuestiones: la regulación de la materia en sus aspectos “materiales” (horario, cursos, el concepto “social” e “institucional” de la disciplina (en conexión con el resto de las lenguas vivas y, finalmente, los objetivos-contenidos didácticos y las orientaciones metodológicas presentes en los textos oficiales.

  20. Estudio comparativo de la cultura organizacional

    Directory of Open Access Journals (Sweden)

    Góngora, Norberto H.

    2014-12-01

    Full Text Available El presente trabajo da a conocer resultados parciales de la investigación realizada en el marco del proyecto acreditado “Estudio de las Culturas Organizacionales como determinantes de las personas que trabajan en ellas”, cuyo objetivo general es corroborar las características de cada tipo cultural descriptas en la teoría.Los datos que se exponen son el resultado de varios proyectos de investigación desarrollados utilizando la misma metodología. Se ha obtenido gran cantidad de información para analizar variables asociadas al análisis de la cultura organizacional y así, clasificar cada caso en un tipo cultural. Luego, a partir de la base de datos obtenida se han comparado los datos con las características que describe la teoría, a fin de verificarlos o ponerlos en discusión.La conclusión más importante es que la cultura incide en múltiples aspectos de las conductas de las personas y muchos fenómenos, entre ellos los estudiados en este trabajo, así lo demuestran. Es fundamental estudiar este aspecto de las organizaciones en profundidad y no sólo clasificarlas en uno u otro tipo cultural, ya que existen relaciones entre variables que brindan mayor información que resulta relevante para comprender el comportamiento de las personas.

  1. Un estudio de resentimiento en ancianos institucionalizados

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    Dante Gazzolo D.

    1987-06-01

    Full Text Available El presente estudio es una investigación realizada por los Servicios de Psicología del Instituto Nacional de Salud Mental "Honorio Delgado - Hideyo Noguchi", y del Hogar "lgnacia Rodulfa V da. de Canevaro", a través del cual se intenta un acercamiento al devenir psicológico del anciano institucionalizado. Este, al residir permanentemente al interior de un asilo, manifiesta sentimientos de frustración, fracaso y resentimiento, entre otros. Se intenta determinar el grado de: resentimiento que se hace presente en ancianos institucionalizados, tomando en cuenta variables diferenciadoras como condición de vivienda (nivel socio-económico, sexo, edad, estado civil, grado de instrucción, actividad laboral, presencia  o ausencia de hijos, y tiempo de residencia en el asilo.Para tal fin, se evaluó a una muestra de 53  ancianos, 32 mujeres y 21 hombres, 18 de nivel socio-económico alto, y 35 de nivel socio-económico bajo, a través de un Inventario de Actitudes hacia la Vida, encontrando que los sujetos de nivel socio-económico bajo presentan mayores grados de resentimiento que los sujetos de nivel socio-económico alto, observándose, de otro lado, mayores niveles de resentimiento en el sexo masculino que en el femenino.

  2. Anorexia nervosa: un estudio de casos

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    Lillyana Zusman Tinman

    2013-09-01

    Full Text Available La Anorexia Nervosa es un trastorno de alimentación que se define (etimológicamente como una "pérdida nerviosa del apetito". Se caracteriza por la actitud consciente, voluntaria y rotunda de los sujetos  de tener un exceso de peso que intentan modificar por vía de la inanición. A partir del estudio de casos, se propone la distinción entre una Anorexia Nervosa Estructural -aquella en la que predomina el conflicto intrapsíquico primario y arcaico, y que manifiesta una conducta aislada y retraída- y una Anorexia Nervosa Reactiva, aquella en la que predomina un conflicto intrafamiliar algo más posterior y, por lo tanto, una personalidad más abierta y compatible con el medio. Anorexia Nervosa is an Eating Disorder defined (etimoligacally as a "nervouse loss of apetite". It is characterized by a conscious, voluntary and categoric attitude of the sick patient to refrain from eating. He/she has a firm conviction of having excess weight which they try to modify by starvation. Through a case study, a distinction between two types of Anorexia Nervosa is proposed: a Structural Anorexia Nervosa -in which an intrapsyhic, primary, arcaic conflic prevails, leading to an isolated, withdrawal conduct- and a Reactive Anorexia Nervosa, in which a later intrafamilial conflict prevails leading, therefore, to a more open personality, compatible with the enviroment.

  3. Estudio electrofisiologico en la neuropatia por Vincristina

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    Olga P. Sanz

    1975-12-01

    Full Text Available Diez pacientes afectados por diversas patologías que requerían tratamiento crónico con Vincristina, fueron sometidos a estudios electrofisiológicos en los que se valoró: el número de unidades motoras (UM funcionantes en los músculos de la eminencia tenar, los valores de los incrementos medios de UM, velocidad de conducción motora y su latencia residual en el nervio mediano, la velocidad de conducción sensitiva del mismo nervio y el estado de la transmisión neuromuscular. Los valores obtenidos fueron comparados con grupos controles. Los resultados mostraron disminución del número de UM; las UM remanentes presentaron amplitud reducida junto a otras cuyo tamaño no superaba el del grupo control, hecho que sugiere la incapacidad de lograr una reinervación adecuada. Las velocidades de conducción motora y sensitiva mostraron valores diminuídos, con mayor compromiso en los segmentos distales. Junto a estos datos se halló respuesta miasteniforme al estímulo repetitivo. Todos estos resultados permiten postular la existencia de un compromiso de la unidad motora, abarcando todos sus segmentos, en pacientes intoxicados con Vincristina.

  4. ESTUDIOS CURRICULARES Y LA HISTORIA DEL PRESENTE

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    Thomas S. Popkewitz

    2010-01-01

    Full Text Available El campo de la historia del currículum, así como más ampliamente el de la historia de la educación, está organizado por tradiciones de historia intelectual y social; antes más preocupado por la organización y el cambio en las ideas; más recientemente con las ideas como representación institucional y cambios sociales encontrados en la política y en los actuales desarrollos programáticos. Este artículo formula una perspectiva sobre el currículum como una historia del presente. Se considera el currículum como "ordenanzas de conversión" pensando el currículum como narrativas de pertenencia nacional y ciencia emparejada con temas de salvación que generan tesis culturales sobre modos de vida. La sección segunda habla de la Educación Americana Progresiva en un reformismo protestante que, cruzado el Atlántico, se preocupa por la cuestión social. La sección final explora las tesis culturales generadas en la formación de las matemáticas, el alfabetismo, y el plan de estudios de música.

  5. Method and apparatus for processing algae

    Science.gov (United States)

    Chew, Geoffrey; Reich, Alton J.; Dykes, Jr., H. Waite; Di Salvo, Roberto

    2012-07-03

    Methods and apparatus for processing algae are described in which a hydrophilic ionic liquid is used to lyse algae cells. The lysate separates into at least two layers including a lipid-containing hydrophobic layer and an ionic liquid-containing hydrophilic layer. A salt or salt solution may be used to remove water from the ionic liquid-containing layer before the ionic liquid is reused. The used salt may also be dried and/or concentrated and reused. The method can operate at relatively low lysis, processing, and recycling temperatures, which minimizes the environmental impact of algae processing while providing reusable biofuels and other useful products.

  6. Aplicação do processo de enfermagem baseado na teoria de OREM: estudo de caso com uma adolescente grávida Aplicación de lo proceso de enfermería basado en la teoría de OREM: estudio de caso en una adolescente embarazada Application of the nursing process based in OREM'S theory: a case study with a pregnant adolescent

    Directory of Open Access Journals (Sweden)

    Gilson de Vasconcelos Torres

    1999-04-01

    Full Text Available Estudo do tipo descritivo com abordagem qualitativa, objetivando a aplicação do processo de enfermagem baseado na Teoria do Autocuidado de Orem, através de um estudo de caso em uma adolescente grávida, buscando identificar os diagnósticos de enfermagem na referida cliente, baseado nos diagnósticos de enfermagem da NANDA. Diante dos resultados obtidos identificou-se três diagnósticos: adaptação prejudicada, distúrbio do padrão do sono e processo familiar alterado. Destacou-se a aplicabilidade do processo de enfermagem baseado em Orem e a importância dos diagnósticos de enfermagem na prestação dos cuidados de enfermagem à cliente.Estudio de tipo descriptivo con un enfoque cualitativo, cuyo objetivo fue aplicar el proceso de enfermería utilizando la Teoría del Autocuidado de OREM, a través de un estudio de caso en una adolescente embarazada, buscando identificar los diagnósticos de la cliente, en base a los diagnósticos de la NANDA. De acuerdo con los resultados encontrados, se identificaron tres diagnósticos: adaptación perjudicada, disturbio del sueño y alteración del proceso familiar, fue relevante la aplicación del proceso de enfermería de Orem y la importancia de los diagnósticos de enfermería para dar los cuidados de enfermería a la cliente.This is a descriptive study with a qualitative approach that aimed at applying the nursing process based in Orem Self-Care Theory, through a case study with a pregnant adolescent in order to identify the nursing diagnosis in the above mentioned clients, based on NANDA'S nursing diagnoses. Results obtained identified three nursing diagnoses: prejudiced adaptation, sleep disturb and familiar change process. The application of the nursing process based in Orem and the importance of the diagnosis identified for clients nursing care were evidenced.

  7. Technical study for the automation and control of processes of the chemical processing plant for liquid radioactive waste at Racso Nuclear Center; Estudio tecnico para la automatizacion y control de procesos de la planta de tratamiento quimico de los residuos liquidos radiactivos del Centro Nuclear Racso

    Energy Technology Data Exchange (ETDEWEB)

    Quevedo D, M; Ayala S, A

    1997-07-01

    The purpose of this study is to introduce the development of an automation and control system in a chemical processing plant for liquid radioactive waste of low and medium activity. The control system established for the chemical processing plant at RACSO Nuclear Center is described. It is an on-off sequential type system with feedback. This type of control has been chosen according to the volumes to be treated at the plant as processing is carried out by batches. The system will be governed by a programmable controller (PLC), modular, with a minimum of 24 digital inputs, 01 analog input, 16 digital outputs and 01 analog input. Digital inputs and outputs are specifically found at the level sensors of the tanks and at the solenoid-type electro valve control. Analog inputs and outputs have been considered at the pH control. The comprehensive system has been divided into three control bonds, The bonds considered for the operation of the plant are described, the plant has storing, fitting, processing and clarifying tanks. National Instruments' Lookout software has been used for simulation, constituting an important tool not only for a design phase but also for a practical one since this software will be used as SCADA system. Finally, the advantages and benefits of this automation system are analyzed, radiation doses received by occupationally exposed workers are reduced and reliability on the operation on the system is increased. (authors)

  8. Los estudios culturales en Centroamérica.

    OpenAIRE

    Fumero, Patricia

    2012-01-01

    En el presente artículo se considerará el inicio de los estudios culturales en Centroamérica y las diversas corrientes que influyeron en este cambio. Posteriormente, se analizará la problemática que supone el estudio del istmo, en términos de su diversidad y del desarrollo de adecuadas perspectivas comparativas. Por último, se revisarán los avances logrados por los estudios literarios y los desafíos planteados en el futuro inmediato.

  9. Recursos bibliográgicos sobre los estudios en italiano

    OpenAIRE

    Caprara, Giovanni

    2009-01-01

    Este artículo bibliográfico busca ofrecer una visión panorámica actualizada (y representativa) de los Estudios de Traducción en lengua italiana. En ella se clasifican por temas objeto de estudio toda una serie de obras (libros, monografías colectivas, etc.) que inciden sobre el estudio de la Traducción e Interpretación desde la perspectiva de la lengua y la cultura italianas o que, aunque hayan sido realizados por autores extranjeros, su publicación utiliza como lengua vehicular de comunic...

  10. Estudio comparativo entre SIG propietario y SIG libre

    OpenAIRE

    Mesa Díaz, Juan Ramón

    2008-01-01

    Estudio comparativo entre SIG propietario y SIG libre, focalizado en los casos particulares de Geomedia Pro (SIG Propietario) y gvSIG (SIG Libre). En el estudio se procede a determinar cuáles son los aspectos destacables de un SIG, para poder evaluarlos, posteriormente, en los dos SIG objeto del estudio y obtener una ponderación definitoria de cada SIG. A continuación, algunos de los aspectos evaluados en cada SIG: interoperabilidad, conexión a bases de datos espaciales, aspectos económ...

  11. Xenodiagnostico, hemocultura e teste de lise mediada pelo complemento, como critérios de seleção de pacientes chagásicos crônicos para quimioterapia Xenodiagnosis, hemoculture and complement mediated lysis tests as criteria for selection of chronic chagasic patients for chemotherapy

    Directory of Open Access Journals (Sweden)

    Vera Lúcia Pereira

    1989-10-01

    Full Text Available O tratamento etiológico da doença de Chagas é iniciado geralmente apenas quando se dispõe de um diagnóstico parasitológico positivo. Na tentativa de aumentar o número de candidatos assim selecionados para o tratamento específico, estudamos 36 pacientes chagásicos crônicos associando o xenodiagnóstico, a hemocultura e o teste de lise mediada pelo complemento, em duas séries sucessivas, intercaladas de um mínimo de 60 dias. A sensibilidade do xenodiagnóstico e da hemocultura foi respectivamente de 30,5% e de 8,3% na primeira série e de 36,1% e de 19,4% nas duas séries. Foram positivos, em pelo menos uma das duas provas, 17 (47,2% dos pacientes. Destes, entretanto, somente 9 (53,0% mostraram teste de lise constantemente positivo enquanto que em 5 (29,4% o teste foi negativo e 3 (17,6% apresentaram resultados ora positivos, ora negativos. Nos pacientes com provas parasitológicas negativas, o teste de lise foi positivo em 4 (15,8%, negativo em 9 (47,4% e discordante em 6 (31,5%. Assim, o teste de lise mediada pelo complemento não se constitue em bom método de triagem de candidatos ao tratamento. Apesar da baixa sensibilidade, as provas parasitológicas ainda constituem o instrumento mais seguro para o clínico.Normally specific treatment of chronic Chagas' disease begins only after a positive parasitological diagnosis has been established. Xenodiagnosis, hemoculture and complement mediated lysis were associated, and repeated, as an attempt to increase the number of selected candidates for specific treatment. Thirty six chronic chagasic patients were submitted to two series of the above tests, with a minimal interval of 60 days. In the first series of tests sensitivity of xenodiagnosis and hemoculture were 30.5% and 8.3% respectively. Processing of a second sample increased sensitivity to 36.1% (xenodiagnosis and 19.4% (hemoculture; 47.2% were shown to be positive by at least one of these tests. From the positive cases, 29.4% were

  12. Implantes dentales en pacientes adultos postrauma dentoalveolar. Estudio descriptivo

    Directory of Open Access Journals (Sweden)

    Edgardo González

    2016-04-01

    Conclusiones: En este estudio se presenta un protocolo establecido y se establece la necesidad de un diagnóstico detallado para planificar la rehabilitación mediante implantes dentales posterior a un trauma con un equipo multidisciplinario.

  13. Automatic Sample and Data Processing in Studies of Calcium Metabolism in Rats; Traitement Automatique des Echantillons et des Donnees dans l'Etude du Metabolisme Calcique chez le Rat; Avtomaticheskaya obrabotka obraztsov i dannykh pri izuchenii obmena kal'tsiya u krysy; Tratamiento Automatico de las Muestras y de los Datos en el Estudio del Metabousmo del Calcio en la Rata

    Energy Technology Data Exchange (ETDEWEB)

    Onkelinx, C.; Richelle, L. J.; Debras, J. [Universite de Liege (Belgium)

    1965-10-15

    The study of calcium metabolism in rats as a function of age or various forms of treatment entails experiments on large numbers of animals. These investigations involve: (i) studying the way in which the serum concentration of a tracer dose of {sup 45}Ca injected intravenously varies as a function of time, and (ii) carrying out measurements of chemical and radiochemical balance. By combining these two types of information and subjecting them to mathematical analysis it is possible to evolve a general model of calcium metabolism. This model can then be used to deduce the size of the exchangeable compartments and the relative importance of the different metabolic paths, such as intestinal absorption, renal and intestinal excretion, and deposition and elimination of bone calcium. The authors' work on these subjects was facilitated by the development of automatic methods for measuring the samples and processing the data, and these methods are the subject of their paper. Processing of samples: the radioactivity measurements are carried out on small samples (20-40 {lambda}) of plasma, removed at repeated intervals, and on total quantities of faeces and urine excreted in a given period. The measuring apparatus used comprises a feed, a low-background anti-coincidence counter and a digital computer; the measurements obtained from the computer are then recorded on a printer. The novel features of the sample preparation techniques used and the performance achieved by the measuring apparatus, are discussed, with special reference to the (statistical) counting conditions, which are checked by the computer each time new measurements have to be calculated. Processing of data: this is done by an IBM-7040 digital computer, into which are fed the programme for the calculation and all the un-corrected experimental data in the form of punched cards, separately for each animal. There are three stages to the data-processing operation, namely: (1) converting the raw data and calculating

  14. El estudio de la cadena productiva del fique

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    Maria Eugenia Morales Rubiano

    2002-09-01

    Full Text Available El estudio recopila los resultados más importantes obtenidos en el trabajo de grado denominado "Estudio de factibilidad de la cadena productiva del fique cultivado en Colombia", el cual tomo como punto de referencia el sector fiquero para estimas la factibilidad de aplicar un esquema de cadena productiva, a través de la estructura y análisis estratégico del sistema.

  15. 白血病合并肿瘤溶解综合征的诊治%Diagnosis and treatment of tumor lysis syndrome associated with leukemia

    Institute of Scientific and Technical Information of China (English)

    秘营昌

    2011-01-01

    肿瘤溶解综合征(TLS)是血液肿瘤科常见的急症,临床表现主要有高尿酸血症、高钾血症、高磷酸血症、低钙血症等,最终导致肾功能衰竭、心律失常、癫痫、神经系统并发症,甚至死亡.目前对TLS诊断、疾病危险度判断、治疗观点较一致,提高了TLS的诊断、治疗水平.%Tumor lysis syndrome (TLS) is a life-threatening oncological emergency characterized by metabolic abnormalities including hyperuricaernia,hyperphosphataemia,hyperkalaemia and hypocalcaemia. These metabolic complications will eventually lead to a series of complications. Now that the diagnosis of TI.S has been clarified, a panel of TLS experts have offered recommendations for evaluation of risks and prophylaxis of TLS to improve the prognosis of patients with TLS.

  16. Use of conivaptan to allow aggressive hydration to prevent tumor lysis syndrome in a pediatric patient with large-cell lymphoma and SIADH.

    Science.gov (United States)

    Rianthavorn, Pornpimol; Cain, Joan P; Turman, Martin A

    2008-08-01

    The available treatment options for hyponatremia secondary to SIADH are limited and not completely effective. Conivaptan is a vasopressin 1a and 2 receptor antagonist recently approved by the US Food and Drug Administration (FDA) for treating euvolemic and hypervolemic hyponatremia in adult patients. However, data on efficacy and safety of conivaptan in pediatrics are limited. We report a case of a 13-year-old boy with extensively metastasized anaplastic large-cell lymphoma. He also developed hyponatremia due to syndrome of inappropriate antidiuretic hormone secretion (SIADH) prior to chemotherapy initiation. SIADH management in this case was complicated when fluid restriction was not safely attainable. Conivaptan played a significant role in this situation by allowing provision of a large amount of intravenous fluid prior to and during induction chemotherapy. It proved to be an important component in preventing uric acid nephropathy/tumor lysis syndrome. Conivaptan induced free-water clearance as indicated by increased urine output and decreased urine osmolality. The patient responded to conivaptan without any adverse effects.

  17. The challenges of treating paraganglioma patients with 177Lu-DOTATATE PRRT: Catecholamine crises, tumor lysis syndrome and the need for modification of treatment protocols

    International Nuclear Information System (INIS)

    Makis, William; Mccann, Karey; Mcewan, Alexander J. B.

    2015-01-01

    A high percentage of paragangliomas express somatostatin receptors that can be utilized for targeted radioisotope therapy. The aim of this study was to describe and discuss the challenges of treating these tumors with 177 Lu-[DOTA0,Tyr3]octreotate (DOTATATE) radioisotope therapy using established protocols. Three paraganglioma patients were treated with 4–5 cycles of 177 Lu-DOTATATE and were evaluated for side effects and response to therapy. Two of the three patients developed severe adverse reactions following their first 177 Lu-DOTATATE treatment. One patient developed a catecholamine crisis and tumor lysis syndrome within hours of treatment, requiring intensive care unit (ICU) support, and another developed a catecholamine crisis 3 days after treatment, requiring hospitalization. The treatment protocols at our institution were subsequently modified by increasing the radioisotope infusion time from 15 to 30 min, as recommended in the literature, to 2–4 h and by reducing the administered dose of 177 Lu-DOTATATE. Subsequent 177 Lu-DOTATATE treatments utilizing the modified protocols were well tolerated, and response to therapy was achieved in all three patients, resulting in significantly improved quality of life. 177 Lu-DOTATATE is an exciting new therapeutic option in the management of paragangliomas; however, current treatment protocols described in the literature may need to be modified by lengthening the infusion time and/or lowering the initial treatment dose to prevent or reduce the severity of adverse reactions

  18. The challenges of treating paraganglioma patients with {sup 177}Lu-DOTATATE PRRT: Catecholamine crises, tumor lysis syndrome and the need for modification of treatment protocols

    Energy Technology Data Exchange (ETDEWEB)

    Makis, William; Mccann, Karey; Mcewan, Alexander J. B. [Dept. of Diagnostic Imaging, Cross Cancer Institute, Alberta (China)

    2015-09-15

    A high percentage of paragangliomas express somatostatin receptors that can be utilized for targeted radioisotope therapy. The aim of this study was to describe and discuss the challenges of treating these tumors with {sup 177}Lu-[DOTA0,Tyr3]octreotate (DOTATATE) radioisotope therapy using established protocols. Three paraganglioma patients were treated with 4–5 cycles of {sup 177}Lu-DOTATATE and were evaluated for side effects and response to therapy. Two of the three patients developed severe adverse reactions following their first {sup 177}Lu-DOTATATE treatment. One patient developed a catecholamine crisis and tumor lysis syndrome within hours of treatment, requiring intensive care unit (ICU) support, and another developed a catecholamine crisis 3 days after treatment, requiring hospitalization. The treatment protocols at our institution were subsequently modified by increasing the radioisotope infusion time from 15 to 30 min, as recommended in the literature, to 2–4 h and by reducing the administered dose of {sup 177}Lu-DOTATATE. Subsequent {sup 177}Lu-DOTATATE treatments utilizing the modified protocols were well tolerated, and response to therapy was achieved in all three patients, resulting in significantly improved quality of life. {sup 177}Lu-DOTATATE is an exciting new therapeutic option in the management of paragangliomas; however, current treatment protocols described in the literature may need to be modified by lengthening the infusion time and/or lowering the initial treatment dose to prevent or reduce the severity of adverse reactions.

  19. Las javieradas como caso de estudio

    Directory of Open Access Journals (Sweden)

    Mª Cruz Porcal Gonzalo

    2006-01-01

    Full Text Available A pesar de su todavía modesta participación en la economía y en el empleo regional, el turismo en Navarra ha mostrado durante los últimos años un significativo dinamismo tal y como, por ejemplo, lo testimonia el incremento del número de visitantes y de las empresas turísticas. Favorecido por una serie de circunstancias de índole variada, este desarrollo turístico se sustenta en una gran diversidad geográfica y cultural patente en los paisajes, el arte, las fiestas, la gastronomía, etc. Pero Navarra es, a su vez, una región que secularmente ha otorgado un gran peso a la tradición y a las creencias religiosas. En este contexto, algunas de las numerosas acciones emprendidas por la administración regional para dinamizar el sector turístico se han encaminado a revalorizar dos importantes legados culturales: el castillo de Javier y el Camino de Santiago. Se aprecia que ambos se hallan estrechamente relacionados con el turismo cultural, el turismo religioso y las peregrinaciones, unos fenómenos que hunden sus raíces en tiempos pasados pero que continúan teniendo en la actualidad una indudable relevancia a escala mundial. La intención primordial del presente trabajo es analizar el turismo de peregrinaciones en Navarra, más concretamente, el que tiene como destino del viaje la cuna de San Francisco Javier. Con dicho afán, se realiza una reflexión conceptual previa y un breve repaso de las fiestas y tradiciones religiosas que forman parte del patrimonio cultural intangible de Navarra, haciendo particular hincapié en las romerías y en el Camino de Santiago. Al hilo de ello, se afronta, a continuación, el estudio de la peregrinación más singular y populosa de Navarra: la Javierada. Su origen, sus principales rasgos, el perfil y las motivaciones de los peregrinos, los caracteres e hitos más importantes del recorrido, las peculiaridades del destino —entendido como lugar sagrado y espacio turístico— constituyen las principales

  20. Improvement of the obtention process of {sup 131} I of ININ through studies of equipment design and process parameters fitting in the simulator; Perfeccionamiento del proceso de obtencion del {sup 131} I del ININ mediante estudios de diseno del equipo y ajustes de parametros del proceso en el simulador

    Energy Technology Data Exchange (ETDEWEB)

    Zepeda M, M C

    2006-07-01

    The main objective of the work was to achieve a high yield of the reaction that happens among the vapors of {sup 131} I and the NaOH to obtain sodium iodide (Na{sup 131} I) in solution optimizing the contact time of the molecules in gaseous state of {sup 131} I with the NaOH molecules through the bubbling velocity of the {sup 131} I in the solution of NaOH 0.1 N, as well as the operation temperature during the distillation process by dry via starting from tellurium dioxide (TeO{sub 2}) with the purpose of that the {sup 131} I that is obtained in the form of Na{sup 131} I solution presents the necessary quality that allows to distribute it in the market. The particular objectives were: a) To improve the distillation equipment used for the obtaining of {sup 131} I starting from irradiated tellurium dioxide redrawing the dilution system with the purpose of recovering the {sup 131} I in a minimum volume of sodium hydroxide (NaOH). b) To achieve a bigger time of contact among the molecules of {sup 131} I in gaseous state with the molecules of NaOH in solution through the bubbling velocity and of the redrawing of the dilution system. c) To implement the production of {sup 131} I by dry distillation via starting from tellurium dioxide, obtaining it with a high radionuclide and radiochemical purity. d) To obtain {sup 131} I with the necessary specifications that it marks the pharmacopoeia and it can be used with medical ends. (Author)

  1. ESTUDIOS DE CASOS Y CONTROLES: A PROPÓSITO DE DOS ESTUDIOS EN CIMEL

    Directory of Open Access Journals (Sweden)

    Francisco Javier Bonilla-Escobar

    2013-08-01

    Full Text Available Los estudios de casos y controles han existido desde el siglo XIX con el analisis de Jhon Snow en Inglaterra sobre la fuente del Colera, y han tomado inusitada importancia despues de su puesta en marcha en diversos campos de la ciencia moderna (1, siendo objeto de amplias discusiones metodologicas hace mas de 50 anos. En los dos numeros anteriores de la revista CIMEL, se encuentran dos de estas publicaciones, tituladas: Factores asociados al Sindrome de Aspiracion Meconial en el hospital Jose Cayetano Heredia Piura-Peru, de Purizaca y col. (2, y Factores asociados al desarrollo de preeclampsia en un hospital de Piura, Peru, de Benites-Condor y col. (3. Reconociendo la importancia de este tipo de publicaciones y el trabajo requerido para su elaboracion, queremos mencionar aciertos y falencias metodologicas que hemos encontrado en estas publicaciones y las cuales afectan las afirmaciones de los autores

  2. Los estudios sobre paisaje en Estudios Geográficos (1940-2009

    Directory of Open Access Journals (Sweden)

    Arroyo Ilera, Fernando

    2010-12-01

    Full Text Available Not available.Los doscientos sesenta y nueve números editados de esta revista son un excelente banco de pruebas donde observar la evolución y los cambios experimentados por la Geografía española, en los últimos setenta años en los que Estudios Geográficos (EG se viene publicando ininterrumpidamente. La sucesión de temas, su tratamiento y enfoque, las citas bibliográficas, etc., constituyen una excelente referencia de gran interés para cualquier ciencia y, en especial para la nuestra, que ya en otras ocasiones ha sido utilizada para valorar la evolución metodológica de la Geografía.

  3. Produção de protoplastos e lise da parede celular de leveduras utilizando β-1,3 glucanase Protoplasts production and yeast cell wall lysis using β-1,3 glucanase

    Directory of Open Access Journals (Sweden)

    Luciana Francisco Fleuri

    2010-06-01

    Full Text Available O presente trabalho visou a aplicação da β-1,3 glucanase lítica, obtida do microrganismo Cellulosimicrobium cellulans 191, na produção de protoplastos e na lise da parede celular de leveduras. A preparação bruta da enzima foi capaz de lisar as leveduras Kluyveromyces lodderi, Saccharomyces cerevisiae (Fleischmann e Itaiquara, S. cerevisiae KL-88, S. diastaticus NCYC 713, S. cerevisiae NCYC 1001, Candida glabrata NCYC 388, Kluyveromyces marxianus NCYC 587 e Hansenula mrakii NCYC 500. A β-1,3 glucanase purificada foi capaz de lisar as leveduras Saccharomyces cerevisiae KL-88, Saccharomyces capensis, Debaromyces vanriji, Pachysolen tannophillus, Kluyveromyces drosophilarum, Candida glabrata, Hansenula mrakii e Pichia membranaefaciens e formar protoplastos de Saccharomyces cerevisiae KL-88.The aim of this work was the application of lytic β-1,3 glucanase obtained from Cellulosimicrobium cellulans strain 191 in the production of protoplasts and lysis of yeast cell walls. The crude extract demonstrated lysis activity against the yeasts Kluyveromyces lodderi, Saccharomyces cerevisiae (Fleischmann and Itaiquara, S. cerevisiae KL-88, S. diastaticus NCYC 713, S. cerevisiae NCYC 1001, Candida glabrata NCYC 388, Kluyveromyces marxianus NCYC 587, and Hansenula mrakii NCYC 500. The purified β-1,3 glucanase demonstrated lysis activity against the yeasts Saccharomyces cerevisiae KL-88, Saccharomyces capensis, Debaromyces vanriji, Pachysolen tannophillus, Kluyveromyces drosophilarum, Candida glabrata, Hansenula mrakii, and Pichia membranaefaciens, and it was able to produce Saccharomyces cerevisiae KL-88 protoplasts.

  4. Um Estudo do Processo de Certificação ISO no Departamento de Oficinas da Companhia do Metropolitano de São PauloA Study of the ISO Certification Process in the Department of repair shop of the Companhia do Metropolitano in São PauloUn Estudio del Proceso de Certificación ISO en el Departamento de Talleres de la Compañía del Metropolitano de São Paulo

    Directory of Open Access Journals (Sweden)

    NETO, Diamantino Augusto Sardinha

    2004-09-01

    Full Text Available RESUMOEste estudo tem por objetivo investigar como foi a certificação ISO 9000 no Departamento de Oficinas da Companhia do Metropolitano de São Paulo – Metrô, no processo do Material Rodante, enfocando a avaliação do processo vivenciado pelos participantes diretos. A pesquisa foi realizada entre os funcionários do Departamento para estudar as impressões do grupo sobre a certificação e seus resultados. A análise dos dados permitiu verificar a percepção dos funcionários sobre a implantação e identificar mudanças ocorridas, principalmente no que tange à documentação, organização e procedimentos de manutenção. Com este trabalho pretende-se contribuir para os interessados na certificação nas normas ISO 9000 e seus efeitos nas empresas, especialmente nas de economia mista, como a que foi objeto deste estudo.ABSTRACTThis study aims to investigate how was the ISO 9002 certification process in the Repair Department of the Companhia do Metropolitano de São Paulo – Metrô, in the Wheeling Material process, focusing the evaluation of the process lived by the direct participants. The survey has been performed among the Department employees’ in order to study the group’s impression on the certification and its results. The data analysis allowed verifying the employees’ perception on the process and identifying the changes with the certification mainly with reference to documentation, organization and maintenance procedures. With this work we intend to contribute for further studies on certification in accordance with ISO 9000 requirements and its effects on the organizations, specially the ones with mixed economy, like this that was the subject of this research.RESUMENEste estudio tiene por finalidad investigar como fue la certificación ISO 9000 en el Departamento de Talleres de la Compañía del Metropolitano de São Paulo – Metro, en el proceso del Material Rodante, enfocando la evaluación del proceso vivido por los

  5. Anti-PD-L1/TGFβR2 (M7824) fusion protein induces immunogenic modulation of human urothelial carcinoma cell lines, rendering them more susceptible to immune-mediated recognition and lysis.

    Science.gov (United States)

    Grenga, Italia; Donahue, Renee N; Gargulak, Morgan L; Lepone, Lauren M; Roselli, Mario; Bilusic, Marijo; Schlom, Jeffrey

    2018-03-01

    Avelumab has recently been approved by the Food and Drug Administration for the therapy of Merkel cell carcinoma and urothelial carcinoma. M7824 is a novel first-in-class bifunctional fusion protein comprising a monoclonal antibody against programmed death-ligand 1 (PD-L1, avelumab), fused to the extracellular domain of human transforming growth factor beta (TGFβ) receptor 2, which functions as a TGFβ "trap." Advanced urothelial tumors have been shown to express TGFβ, which possesses immunosuppressive properties that promote cancer progression and metastasis. The rationale for a combined molecule is to block the PD-1/PD-L1 interaction between tumor cells and immune cell infiltrate and simultaneously reduce or eliminate TGFβ from the tumor microenvironment. In this study, we explored the effect of M7824 on invasive urothelial carcinoma cell lines. Human urothelial (transitional cell) carcinoma cell lines HTB-4, HTB-1, and HTB-5 were treated with M7824, M7824mut (M7824 that is mutated in the anti-PD-L1 portion of the molecule and thus does not bind PD-L1), anti-PD-L1 (avelumab), or IgG1 isotype control monoclonal antibody, and were assessed for gene expression, cell-surface phenotype, and sensitivity to lysis by TRAIL, antigen-specific cytotoxic T lymphocytes and natural killer cells. M7824 retains the ability to mediate antibody-dependent cellular cytotoxicity of tumor cells, although in some cases to a lesser extent than anti-PD-L1. However, compared to anti-PD-L1, M7824 increases (A) gene expression of molecules involved in T-cell trafficking in the tumor (e.g., CXCL11), (B) TRAIL-mediated tumor cell lysis, and (C) antigen-specific CD8 + T-cell-mediated lysis of tumor cells. These studies demonstrate the immunomodulatory properties of M7824 on both tumor cell phenotype and immune-mediated lysis. Compared to anti-PD-L1 or M7824mut, M7824 induces immunogenic modulation of urothelial carcinoma cell lines, rendering them more susceptible to immune

  6. β-1,3 Glucanases e quitinases: aplicação na lise de leveduras e inibição de fungos β-1,3 glucanases and chitinases: application in the yeast cell lysis and fungi inhibition

    Directory of Open Access Journals (Sweden)

    Luciana Francisco Fleuri

    2008-08-01

    Full Text Available Objetivou-se, no presente trabalho, a aplicação de β-1,3 glucanases e quitinases da linhagem Cellulosimicrobium cellulans 191 na lise de leveduras e inibição de fungos, respectivamente. O delineamento experimental mostrou que as melhores condições para a lise de Saccharomyces cerevisiae KL-88 pela β-1,3 glucanase foi pH 6,5 e 35ºC. As células de leveduras incubadas por 10 h em frascos sem agitação mostraram-se mais susceptíveis à lise pela ação da enzima. Foi obtido maior lise da levedura quando a suspensão de células foi submetida ao tratamento com β-1,3 glucanase e cisteína 1mM. A enzima invertase intracelular ou ligada à célula de S. cerevisiae KL-88 e K. marxianus NCYC 587 foi extraída após tratamento da suspensão celular com β-1,3 glucanase, sendo que o tratamento prévio das leveduras com a enzima aumentou a susceptibilidade das células à lise com ultra-som. A preparação de quitinase foi capaz de formar halos de inibição de alguns fungos.The aim of this work was the application of β-1,3 glucanases and chitinases by Cellulosimicrobium cellulans 191 strain on yeast cell lysis and fungi inhibition, respectively. The experimental design study showed that the best conditions to Saccharomyces cerevisiae KL-88 lysis by β-1,3 glucanase extract were pH 6,5 and 35ºC. This study also demonstrated that the yeast cells were more susceptible to lysis after 10 h of cultivation in flasks without agitation. Lysis activity was increased when S. cerevisiae KL-88 cell suspension was treated with β-1,3 glucanase and cystein 1mM. The enzyme invertase of S. cerevisiae KL-88 and Kluyveromyces marxianus NCYC 587 was extracted after treatment of cell suspension with β-1,3 glucanase and the previous treatment of yeasts with the enzyme, increased the susceptibility to lysis when ultrasonic treatment was used. The chitinase presented growth inhibition halos for some of the fungi.

  7. ESTUDIOS DE PERIODISMO EN ARGENTINA: ANTECEDENTES E INTERROGANTES

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    Adriana Amado Suárez

    2014-06-01

    Full Text Available Este artículo presenta de forma resumida una revisión de antecedentes de los estudios empíricos de periodismo en Argentina. En las publicaciones de los últimos años los investigadores coincidían en señalar falta de datos de la profesión y el escaso desarrollo de los marcos teóricos afines a los estudios de periodismo. La investigación local priorizó abordajes y métodos que no se ocupan del conjunto de los periodistas. Antes bien, buena parte de la investigación confunde análisis de los medios y los mensajes con el estudio del periodismo y sigue sin brindar datos que permitan conocer las condiciones de trabajo y el perfil profesional de los periodistas argentinos.

  8. Addition of a sequence from α2-antiplasmin transforms human serum albumin into a blood clot component that speeds clot lysis

    Directory of Open Access Journals (Sweden)

    Gataiance Sharon

    2009-03-01

    Full Text Available Abstract Background The plasma protein α2-antiplasmin (α2AP is cross-linked to fibrin in blood clots by the transglutaminase factor XIIIa, and in that location retards clot lysis. Competition for this effect could be clinically useful in patients with thrombosis. We hypothesized that fusion of N-terminal portions of α2-antiplasmin to human serum albumin (HSA and production of the chimeric proteins in Pichia pastoris yeast would produce a stable and effective competitor protein. Results Fusion protein α2AP(13-42-HSA was efficiently secreted from transformed yeast and purified preparations contained within a mixed population the full-length intact form, while fusions with longer α2AP moieties were inefficiently secreted and/or degraded. The α2AP(13-42-HSA protein, but not recombinant HSA, was cross-linked to both chemical lysine donors and fibrin or fibrinogen by factor XIIIa, although with less rapid kinetics than native α2AP. Excess α2AP(13-42-HSA competed with α2AP for cross-linking to chemical lysine donors more effectively than a synthetic α2AP(13-42 peptide, and reduced the α2AP-dependent resistance to fibrinolysis of plasma clots equally effectively as the peptide. Native α2AP was found in in vivo clots in rabbits to a greater extent than α2AP(13-42, however. Conclusion In this first report of transfer of transglutamination substrate status from one plasma protein to another, fusion protein α2AP(13-42-HSA was shown to satisfy initial requirements for a long-lasting, well-tolerated competitive inhibitor of α2-antiplasmin predicted to act in a clot-localized manner.

  9. Performance assessment of two lysis methods for direct identification of yeasts from clinical blood cultures using MALDI-TOF mass spectrometry.

    Science.gov (United States)

    Jeddi, Fakhri; Yapo-Kouadio, Gisèle Cha; Normand, Anne-Cécile; Cassagne, Carole; Marty, Pierre; Piarroux, Renaud

    2017-02-01

    In cases of fungal infection of the bloodstream, rapid species identification is crucial to provide adapted therapy and thereby ameliorate patient outcome. Currently, the commercial Sepsityper kit and the sodium-dodecyl sulfate (SDS) method coupled with MALDI-TOF mass spectrometry are the most commonly reported lysis protocols for direct identification of fungi from positive blood culture vials. However, the performance of these two protocols has never been compared on clinical samples. Accordingly, we performed a two-step survey on two distinct panels of clinical positive blood culture vials to identify the most efficient protocol, establish an appropriate log score (LS) cut-off, and validate the best method. We first compared the performance of the Sepsityper and the SDS protocols on 71 clinical samples. For 69 monomicrobial samples, mass spectrometry LS values were significantly higher with the SDS protocol than with the Sepsityper method (P < .0001), especially when the best score of four deposited spots was considered. Next, we established the LS cut-off for accurate identification at 1.7, based on specimen DNA sequence data. Using this LS cut-off, 66 (95.6%) and 46 (66.6%) isolates were correctly identified at the species level with the SDS and the Sepsityper protocols, respectively. In the second arm of the survey, we validated the SDS protocol on an additional panel of 94 clinical samples. Ninety-two (98.9%) of 93 monomicrobial samples were correctly identified at the species level (median LS = 2.061). Overall, our data suggest that the SDS method yields more accurate species identification of yeasts, than the Sepsityper protocol. © The Author 2016. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  10. Prevalence of Complement-Mediated Cell Lysis-like Gene (sicG) in Streptococcus dysgalactiae subsp. equisimilis Isolates From Japan (2014-2016).

    Science.gov (United States)

    Takahashi, Takashi; Fujita, Tomohiro; Shibayama, Akiyoshi; Tsuyuki, Yuzo; Yoshida, Haruno

    2017-07-01

    Streptococcus dysgalactiae subsp. equisimilis (SDSE; a β-hemolytic streptococcus of human or animal origin) infections are emerging worldwide. We evaluated the clonal distribution of complement-mediated cell lysis-like gene (sicG) among SDSE isolates from three central prefectures of Japan. Group G/C β-hemolytic streptococci were collected from three institutions from April 2014 to March 2016. Fifty-five strains (52 from humans and three from animals) were identified as SDSE on the basis of 16S rRNA sequencing data.; they were obtained from 25 sterile (blood, joint fluid, and cerebrospinal fluid) and 30 non-sterile (skin-, respiratory tract-, and genitourinary tract-origin) samples. emm genotyping, multilocus sequence typing, sicG amplification/sequencing, and random amplified polymorphic DNA (RAPD) analysis of sicG-positive strains were performed. sicG was detected in 30.9% of the isolates (16 human and one canine) and the genes from the 16 human samples (blood, 10; open pus, 3; sputum, 2; throat swab, 1) and one canine sample (open pus) showed the same sequence pattern. All sicG-harboring isolates belonged to clonal complex (CC) 17, and the most prevalent emm type was stG6792 (82.4%). There was a significant association between sicG presence and the development of skin/soft tissue infections. CC17 isolates with sicG could be divided into three subtypes by RAPD analysis. CC17 SDSE harboring sicG might have spread into three closely-related prefectures in central Japan during 2014-2016. Clonal analysis of isolates from other areas might be needed to monitor potentially virulent strains in humans and animals. © The Korean Society for Laboratory Medicine

  11. Excess sludge reduction in activated sludge processes by integrating ultrasound treatment

    International Nuclear Information System (INIS)

    Perez-Elvira, S.; Fdz-Polanco, M.; Plaza, F. I.; Garralon, G.; Fdz-Polanco, F.

    2009-01-01

    Biological sludge produced in the activated sludge process can be minimised modifying the water line, the sludge line or the final disposal strategy. Selecting the water line the general idea is to reduce the sludge producing the yield coefficient by means of the called lysis cryptic growth process. The main techniques referenced in literature are onization, chlorination and chemical and heat treatment. Ultrasounds are widely used to increase anaerobic biodegradability but are not reported as system to control excess sludge production. (Author)

  12. Process Innovation in Local Governments: an empirical study of continuous improvement effortA Inovação de Processos nas Administrações Locais: um estudo empírico de seu esforço de melhoria contínuaInnovación de Procesos de Administraciones Locales: un estudio empírico sobre su esfuerzo de mejora continua

    Directory of Open Access Journals (Sweden)

    SUÁREZ-BARRAZA, Manuel Francisco

    2013-06-01

    Full Text Available ABSTRACTThis article seeks to explore and understand how applies the process innovation (PI in Spanish local administrations over the years. Therefore, we conducted a qualitative study with a case study research method of four Spanish municipalities, using the following strategies for data gathering: direct observation, participatory observation, document analysis, and semi-structures in-depth interviews. Theories or schools of thought in which the study is based Public Management and Operations Management in the field of Process Innovation. The findings indicate that the implementation of the PI address in local governments is presented through a series of evolutionary stages and improvement activities. The impact in the different processes and public services has been beneficial in terms of internal performance and satisfaction to the citizen. Therefore, when such public institutions have had the will and the commitment of politicians and senior technicians to innovate their work processes, that effort has been the practice of steadily over the years. It is one of the first studies to explore the application of Process Innovation in public management of local governments.RESUMOO presente artigo busca explorar e compreender como se aplica a Inovação de Processos (IP em administrações locais espanholas ao longo dos anos. Para isso, realizou-se um estudo qualitativo com o método de caso de quatro prefeituras (ayuntamientos espanholas, utilizando para a coleta de dados as seguintes estratégias: observação direta, observação participativa, análise documental e entrevistas semiestruturadas em profundidade. As teorias ou escolas de pensamento sobre as quais o estudo é sustentado incluem a administração pública e a gestão de operações em seu campo de inovação de processos. Os resultados indicam que a aplicação de IP nos governos locais é apresentada por meio de uma série de etapas evolutivas e atividades de melhoria. O impacto

  13. Estudio molecular de pacientes colombianos afectados por enanismo esencial

    OpenAIRE

    Navarrete Vargas, Julie Viviana

    2017-01-01

    Los síndromes de enanismo esencial microcefálico son un grupo de enfermedades monogénicas infrecuentes que se caracterizan principalmente por talla baja extrema proporcionada de inicio prenatal y microcefalia severa. En los pacientes que formaron parte del presente estudio se investigaron variantes en el gen PCNT debido a que presentaban hallazgos clínicos compatibles con el síndrome MOPD II (enanismo esencial osteodisplásico microcefálico tipo II). Posteriormente, se amplió el estudio con...

  14. Estudio de factibilidad para la implantación de un restaurante de cocina en Quito

    OpenAIRE

    Abril Donoso, Aníbal Trosky

    2007-01-01

    Componentes y sustentos técnicos. Identificación y descripción de la empresa. Análisis de la industria o sector. Productos y servicos de la empresa. Datos importantes para entrar al mercado. Estudio de mercado. Objetivs del estudio de mercado. Investigación del mercado objetivo. Estudio técnico. Objetivos del estudio técnico. Localización del proyecto. Ingeniería del proyecto. Organización y administración. Estudio financiero. Objetivos de estudio financiero. Sistema contable de la empresa. C...

  15. ¿Quién domina los procesos territoriales? Importancia de los diferentes capitales para un desarrollo sustentable. Caso de estudio: Partido de Balcarce, Argentina // Who dominates the territorial processes?Importance of the different capitals for sustainable development. Case study: Balcarce, Argentina

    Directory of Open Access Journals (Sweden)

    Alejandra Auer

    2017-06-01

    Full Text Available En las últimas décadasestán teniendo lugaren Argentina procesos territorialescomo la agriculturización (intensificación y avance de la agricultura sobre otros usos de la tierra yel desarrollo de planes de ordenamiento territorial rural. En ambos casos los agentes del territorio afectan la evolución de estos procesos y a la vez son afectados por los mismos. Este estudio analizaambos procesos en el Partido de Balcarce (Argentina bajo el enfoque de campos sociales de Pierre Bourdieu. Se observa que la posición de los agentes dentro del campo no solo está determinada por el volumen total del capital que poseen, sino también por la estructura de dicho capital y por el capital dominante dentro del campo, afectandoesto las relaciones de poder entre agentes. Si bien el capital que rige la evolución de ambos procesos es el económico, se observa queel capital social cumple un rol estratégico dentro del campo de la comunidad rural y el capital simbólico en el campo delordenamiento territorial.

  16. UN MODELO LÓGICO-FORMAL PARA EL ESTUDIO DE LOS ARGUMENTOS EMOCIONALES EN LOS PROCESOS DE CONSTRUCCIÓN DE ACUERDOS/ A LOGICAL-FORMAL MODEL FOR THE STUDY OF EMOTIONAL ARGUMENTS IN NEGOTIATION PROCESSES/ UM MODELO LÓGICO-FORMAL PARA O ESTUDO DOS ARGUMENTOS EMOCIONAIS NOS PROCESSOS DE CONSTRUÇÃO DE ACORDOS

    Directory of Open Access Journals (Sweden)

    Antonio Aguilera Ontiveros

    2011-12-01

    Full Text Available Los argumentos son parte de un proceso comunicativo con el cual se trata de incidir en la acción de otros. Gilbert (1994 identifica cuatro modos de argumentación: el modo lógico, el modo emocional, el modo visceral y el modo kisceral. Siguiendo la línea de investigación en psicología computacional marcada por Ortony, Clore y Collins (1988 y el modelo de resolución de conflictos usando negociaciones basadas en argumentos propuesto por Jung y Tambe (2001, este trabajo presenta un modelo lógico-formal para el estudio de un modo concreto de argumentos emocionales dentro del contexto de formación de consensos enmarcado en un proceso de negociación/coordinación. Se discuten sus implicaciones en los modelos cognitivos emocionales basados en el proceso de apreciación/evaluación de la emoción.

  17. El fenómeno administrativo como objeto de estudio

    Directory of Open Access Journals (Sweden)

    Carlos Ramírez Cardona

    2013-07-01

    Full Text Available El perfil de la ciencia o de las ciencias llamadas administrativas resulta confuso cuan teoría general y el objeto de estudio de otras disciplinas relacionadas con el proceso administrativo o que constituyen campos de aplicación de principios y técnicas de la teoría general.

  18. HÁBITOS DE ESTUDIO VS. FRACASO ACADÉMICO

    Directory of Open Access Journals (Sweden)

    Martha Rocío Torres Narváez

    2009-01-01

    Full Text Available El objetivo del artículo es exponer algunas estrategias de apoyo pedagógico que, articuladas con la estructura curricular del Programa de Fisioterapia de la Universidad del Rosario, apoyan el proceso de aprendizaje de los estudiantes a partir de las categorías analizadas con la aplicación del instrumento "Inventario de hábitos de estudio" a los estudiantes que cursaron una de las asignaturas de mayor fracaso académico. Los hábitos de estudio tienen una implicación en el rendimiento académico y esto influye en cómo se enfrenta el reto de asumir las responsabilidades de ser universitario. Como parte de la metodología, se realizó un análisis de los resultados de la aplicación del inventario antes nombrado, en aras de identificar y replantear las estrategias pedagógicas. Para esto se revisaron los planes de asignaturas y se determinaron las actividades extracurriculares que se diseñaron para abordar la problemática de la deserción estudiantil relacionada con el fracaso académico. El estudio realizado determinó la importancia del desarrollo de habilidades o hábitos de estudio apropiados para el buen desempeño del estudiante universitario. Además, comprobó que deben considerarse en el entorno universitario la cultura de aprendizaje en el proceso de formación, las habilidades de trabajo en equipo, la apropiación y el desarrollo de conocimiento, así como las buenas relaciones interpersonales, para disminuir el fracaso académico y mejorar los hábitos de estudio.

  19. Method and apparatus for lysing and processing algae

    Science.gov (United States)

    Chew, Geoffrey; Reich, Alton J.; Dykes, Jr., H. Waite H.; Di Salvo, Roberto

    2013-03-05

    Methods and apparatus for processing algae are described in which a hydrophilic ionic liquid is used to lyse algae cells at lower temperatures than existing algae processing methods. A salt or salt solution is used as a separation agent and to remove water from the ionic liquid, allowing the ionic liquid to be reused. The used salt may be dried or concentrated and reused. The relatively low lysis temperatures and recycling of the ionic liquid and salt reduce the environmental impact of the algae processing while providing biofuels and other useful products.

  20. Urolitiasis obstructiva en ovinos: estudio de caso Urolitiasis obstructiva en ovinos: estudio de caso

    Directory of Open Access Journals (Sweden)

    Abner Josué Gutiérrez Chávez

    2012-02-01

    Full Text Available A 5-months-old, commercial-crossbreed male lamb was remitted to post-morten study. The clinical history about of animal included: signs of severe abdominal distension, anorexia, poliaquiuria, straguria and prolonged urination posture, urine dribbling and blood-tinged urine. Necropsy revealed ascitis and a hemorrhagic fibrine membrane floating in approximately 8 litres of urine caused by a rupture of the urinary bladder in consequence of a total blockage of urethral lumen by multiple small stones localized in the sigmoid flexure of the animal. Obstructive urolithiasis is a common problem in the fed-flocks affecting between 20 % and 30 % of the animals. A diet with adequate mineral balance could reduce the health risk of formation of urinary stones. Un ovino macho de 5 meses de edad fue presentado para su estudio patológico. La historia clínica del animal incluía: postración, dificultad para incorporarse, distensión abdominal severa, pérdida de apetito, estranguria, poliaquiuria y hematuria. A la necropsia se observó la presencia de 8 litros aproximadamente de orina en cavidad abdominal y una membrana fibrino-hemorrágica flotante adherida a la vejiga a nivel de la lesión de ruptura, por una sobre distensión provocada por la presencia obstructiva total de cálculos urinarios localizados en la luz de la uretra a nivel de la flexura sigmoidea del animal. La urolitiasis obstructiva es una patología que se presenta en el 20 % - 30 % de los animales en engorda. Un adecuado balance mineral de la dieta rica en concentrados puede reducir el riesgo de la formación de urolitos.

  1. Lysis of endogenously infected CD4+ T cell blasts by rIL-2 activated autologous natural killer cells from HIV-infected viremic individuals.

    Directory of Open Access Journals (Sweden)

    Manuela Fogli

    2008-07-01

    Full Text Available Understanding the cellular mechanisms that ensure an appropriate innate immune response against viral pathogens is an important challenge of biomedical research. In vitro studies have shown that natural killer (NK cells purified from healthy donors can kill heterologous cell lines or autologous CD4+ T cell blasts exogenously infected with several strains of HIV-1. However, it is not known whether the deleterious effects of high HIV-1 viremia interferes with the NK cell-mediated cytolysis of autologous, endogenously HIV-1-infected CD4+ T cells. Here, we stimulate primary CD4+ T cells, purified ex vivo from HIV-1-infected viremic patients, with PHA and rIL2 (with or without rIL-7. This experimental procedure allows for the significant expansion and isolation of endogenously infected CD4+ T cell blasts detected by intracellular staining of p24 HIV-1 core antigen. We show that, subsequent to the selective down-modulation of MHC class-I (MHC-I molecules, HIV-1-infected p24(pos blasts become partially susceptible to lysis by rIL-2-activated NK cells, while uninfected p24(neg blasts are spared from killing. This NK cell-mediated killing occurs mainly through the NKG2D activation pathway. However, the degree of NK cell cytolytic activity against autologous, endogenously HIV-1-infected CD4+ T cell blasts that down-modulate HLA-A and -B alleles and against heterologous MHC-I(neg cell lines is particularly low. This phenomenon is associated with the defective surface expression and engagement of natural cytotoxicity receptors (NCRs and with the high frequency of the anergic CD56(neg/CD16(pos subsets of highly dysfunctional NK cells from HIV-1-infected viremic patients. Collectively, our data demonstrate that the chronic viral replication of HIV-1 in infected individuals results in several phenotypic and functional aberrancies that interfere with the NK cell-mediated killing of autologous p24(pos blasts derived from primary T cells.

  2. Dextran sulphate crowding and sodium deoxycholate lysis of primary breast fibroblast cells achieve extracellular matrix deposition and decellularization for breast cancer stem cell culture

    Directory of Open Access Journals (Sweden)

    Aroem Naruni

    2016-01-01

    Full Text Available AbstrakLatar belakang: Lingkungan mikro yaitu sel stromal dam matriks ekstraseluler saat ini dinyatakansebagai kontributor dalam perkembangan tumor. Beberapa penelitian telah mengembangkan matriksekstraseluler yang mendukung perkembangan sel in vitro. Matriks ekstraseluler adalah suatu komplekssusunan supramolekuler dari berbagai macam glycoprotein dan proteoglycan. Matriks ekstraselulermenyediakan integritas jaringan, bertindak sebagai scaffold alami tempat sel melekat dan berinteraksiserta berperan sebagai reservoir pertumbuhan sel. Penelitian ini bertujuan untuk mendapatkan deposisidan deselularisasi yang optimal pada matriks ekstraseluler.Metode: Dalam penelitian ini, kami mengembangkan cells crowder untuk meningkatkan deposit matriksekstraseluler dari kultur sel primer fibroblast payudara yang diperoleh dari spesimen hasil operasimammoplasty. Dextran 500 kDa ditambahkan dalam media kultur DMEM lengkap yang telah ditambahkan0.5% FBS dan 100μM L-ascorbic acid 2-phosphate. Setelah tujuh hari, sel dilisis dengan menggunakanSodium Deoxycolate (DOC.Hasil: Deposisi matriks ekstraseluler dan proses deselulerisasi dari sel primer fibroblas payudara dapatterdeteksi dengan menggunakan antibodi Rabbit anti human fibronectin yang selanjutnya ditambahkandengan anti rabbit IgG yang telah dikonjugasi dengan Alexa Fluor 488.Kesimpulan: Penambahan dextran sulfat dan prosesing lysis dengan sodium deoxycolate dapatmeningkatkan deposisi dan menghasilkan deselularisasi matriks ekstraseluler. (Health Science Journalof Indonesia 2015;6:43-7Kata kunci: matriks ekstra selular, kanker mammae, stem cell, sel fibroblast AbstractBackground: The microenvironment including stromal cells and extracellular matrix (ECM is now consideredan active contributor to tumor progression. Certain studies have developed ECM which supports a suitable cellulargrowth in vitro. The ECM is a complex supramolecular assembly of a variety of glycoproteins and proteoglycans

  3. NCG61/24: Plan de Estudios de M??ster en Biomedicina Regenerativa

    OpenAIRE

    Universidad de Granada

    2012-01-01

    Plan de Estudios de M??ster en Biomedicina Regenerativa. Resoluci??n de 5 de marzo de 2012, de la Universidad de Granada, por la que se publica el plan de estudios de M??ster en Biomedicina Regenerativa

  4. CIUDADANOS A PARTIR DEL ESTUDIO GEOGRAFICO

    Directory of Open Access Journals (Sweden)

    Elsa Amanda R. de Toledo

    2007-12-01

    Full Text Available RESUMEN:En este artículo se plantea el concepto de ciudadanía que fundamenta los programas de Ciencias Sociales en la Educación básica de Colombia y su relación con la enseñanza geográfica en el mismo ciclo y en la formación de educadores. Relación que se presenta en la síntesis de algunas experiencias de práctica pedagógica. Establece el vínculo entre la enseñanza de la geografía y la formación de ciudadanos. Así mismo, valora la importancia de tener conciencia del espacio en el que se vive para avanzar en la construcción de ciudadanía y por ende consolidar relaciones más humanas en el espacio urbano. La ciudad se concibe como un espacio pedagógico y didáctico que permite la formación de ciudadanos con pensamiento reflexivo y crítico. PALABRAS-CLAVE: Ciudadanía; práctica pedagógica; escuela; competencias ciudadanas; formación de ciudadanos; espacio público.  ABSTRACT:This article sets up the meaning of citizenship as basis of the Social Studies program, and its relationship with the geography teaching-learning process not only in Elementary School, but also in the Teachers formation process in Colombia. That relationship is evident in the synthesis of diverse pedagogical experiences in the classroom, and reveals the link between the geography learning- teaching exchange and the citizens formation process.Moreover, it emphasises the importance of raising awareness about building up a real citizenship sense, and to consolidate a more humanistic relationship in the urban space. Finally, the city is considered as a pedagogical and didactic context that allows a reflective and critical citizens formation process.KEYWORDS: Citizenship, pedagogical training, school, citizenship competences, citizens formation, public context, space awareness. RÉSUMÉ:Dans cet article se pose le concept de citoyenneté qui fonde les programmes de Sciences Sociales dans l'Éducation basique de la Colombie et sa relation avec l

  5. Same day identification and full panel antimicrobial susceptibility testing of bacteria from positive blood culture bottles made possible by a combined lysis-filtration method with MALDI-TOF VITEK mass spectrometry and the VITEK2 system.

    Directory of Open Access Journals (Sweden)

    Alexandra Machen

    Full Text Available Rapid identification and antimicrobial susceptibility testing of microorganisms causing bloodstream infections or sepsis have the potential to improve patient care. This proof-of-principle study evaluates the Lysis-Filtration Method for identification as well as antimicrobial susceptibility testing of bacteria directly from positive blood culture bottles in a clinical setting. A total of 100 non-duplicated positive blood cultures were tested and 1012 microorganism-antimicrobial combinations were assessed. An aliquot of non-charcoal blood culture broth was incubated with lysis buffer briefly before being filtered and washed. Microorganisms recovered from the filter membrane were first identified by using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight VITEK® Mass Spectrometry (VITEK MS. After quick identification from VITEK MS, filtered microorganisms were inoculated to VITEK®2 system for full panel antimicrobial susceptibility testing analysis. Of 100 bottles tested, the VITEK MS resulted in 94.0% correct organism identification to the species level. Compared to the conventional antimicrobial susceptibility testing methods, direct antimicrobial susceptibility testing from VITEK®2 resulted in 93.5% (946/1012 category agreement of antimicrobials tested, with 3.6% (36/1012 minor error, 1.7% (7/1012 major error, and 1.3% (13/1012 very major error of antimicrobials. The average time to identification and antimicrobial susceptibility testing was 11.4 hours by using the Lysis-Filtration method for both VITEK MS and VITEK®2 compared to 56.3 hours by using conventional methods (p<0.00001. Thus, the same-day results of microorganism identification and antimicrobial susceptibility testing directly from positive blood culture can be achieved and can be used for appropriate antibiotic therapy and antibiotic stewardship.

  6. Same Day Identification and Full Panel Antimicrobial Susceptibility Testing of Bacteria from Positive Blood Culture Bottles Made Possible by a Combined Lysis-Filtration Method with MALDI-TOF VITEK Mass Spectrometry and the VITEK2 System

    Science.gov (United States)

    Machen, Alexandra; Drake, Tim; Wang, Yun F. (Wayne)

    2014-01-01

    Rapid identification and antimicrobial susceptibility testing of microorganisms causing bloodstream infections or sepsis have the potential to improve patient care. This proof-of-principle study evaluates the Lysis-Filtration Method for identification as well as antimicrobial susceptibility testing of bacteria directly from positive blood culture bottles in a clinical setting. A total of 100 non-duplicated positive blood cultures were tested and 1012 microorganism-antimicrobial combinations were assessed. An aliquot of non-charcoal blood culture broth was incubated with lysis buffer briefly before being filtered and washed. Microorganisms recovered from the filter membrane were first identified by using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight VITEK® Mass Spectrometry (VITEK MS). After quick identification from VITEK MS, filtered microorganisms were inoculated to VITEK®2 system for full panel antimicrobial susceptibility testing analysis. Of 100 bottles tested, the VITEK MS resulted in 94.0% correct organism identification to the species level. Compared to the conventional antimicrobial susceptibility testing methods, direct antimicrobial susceptibility testing from VITEK®2 resulted in 93.5% (946/1012) category agreement of antimicrobials tested, with 3.6% (36/1012) minor error, 1.7% (7/1012) major error, and 1.3% (13/1012) very major error of antimicrobials. The average time to identification and antimicrobial susceptibility testing was 11.4 hours by using the Lysis-Filtration method for both VITEK MS and VITEK®2 compared to 56.3 hours by using conventional methods (p<0.00001). Thus, the same-day results of microorganism identification and antimicrobial susceptibility testing directly from positive blood culture can be achieved and can be used for appropriate antibiotic therapy and antibiotic stewardship. PMID:24551067

  7. Same day identification and full panel antimicrobial susceptibility testing of bacteria from positive blood culture bottles made possible by a combined lysis-filtration method with MALDI-TOF VITEK mass spectrometry and the VITEK2 system.

    Science.gov (United States)

    Machen, Alexandra; Drake, Tim; Wang, Yun F Wayne

    2014-01-01

    Rapid identification and antimicrobial susceptibility testing of microorganisms causing bloodstream infections or sepsis have the potential to improve patient care. This proof-of-principle study evaluates the Lysis-Filtration Method for identification as well as antimicrobial susceptibility testing of bacteria directly from positive blood culture bottles in a clinical setting. A total of 100 non-duplicated positive blood cultures were tested and 1012 microorganism-antimicrobial combinations were assessed. An aliquot of non-charcoal blood culture broth was incubated with lysis buffer briefly before being filtered and washed. Microorganisms recovered from the filter membrane were first identified by using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight VITEK® Mass Spectrometry (VITEK MS). After quick identification from VITEK MS, filtered microorganisms were inoculated to VITEK®2 system for full panel antimicrobial susceptibility testing analysis. Of 100 bottles tested, the VITEK MS resulted in 94.0% correct organism identification to the species level. Compared to the conventional antimicrobial susceptibility testing methods, direct antimicrobial susceptibility testing from VITEK®2 resulted in 93.5% (946/1012) category agreement of antimicrobials tested, with 3.6% (36/1012) minor error, 1.7% (7/1012) major error, and 1.3% (13/1012) very major error of antimicrobials. The average time to identification and antimicrobial susceptibility testing was 11.4 hours by using the Lysis-Filtration method for both VITEK MS and VITEK®2 compared to 56.3 hours by using conventional methods (pdirectly from positive blood culture can be achieved and can be used for appropriate antibiotic therapy and antibiotic stewardship.

  8. Rapid Detection and Identification of Candidemia by Direct Blood Culturing on Solid Medium by Use of Lysis-Centrifugation Method Combined with Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)

    Science.gov (United States)

    Idelevich, Evgeny A.; Grünastel, Barbara

    2016-01-01

    ABSTRACT Candida sepsis is a life-threatening condition with increasing prevalence. In this study, direct blood culturing on solid medium using a lysis-centrifugation procedure enabled successful Candida species identification by matrix-assisted laser desorption–ionization time of flight mass spectrometry on average 3.8 h (Sabouraud agar) or 7.4 h (chocolate agar) before the positivity signal for control samples in Bactec mycosis-IC/F or Bactec Plus aerobic/F bottles, respectively. Direct culturing on solid medium accelerated candidemia diagnostics compared to that with automated broth-based systems. PMID:27795344

  9. Rapid Detection and Identification of Candidemia by Direct Blood Culturing on Solid Medium by Use of Lysis-Centrifugation Method Combined with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).

    Science.gov (United States)

    Idelevich, Evgeny A; Grünastel, Barbara; Becker, Karsten

    2017-01-01

    Candida sepsis is a life-threatening condition with increasing prevalence. In this study, direct blood culturing on solid medium using a lysis-centrifugation procedure enabled successful Candida species identification by matrix-assisted laser desorption-ionization time of flight mass spectrometry on average 3.8 h (Sabouraud agar) or 7.4 h (chocolate agar) before the positivity signal for control samples in Bactec mycosis-IC/F or Bactec Plus aerobic/F bottles, respectively. Direct culturing on solid medium accelerated candidemia diagnostics compared to that with automated broth-based systems. Copyright © 2016 American Society for Microbiology.

  10. doméstico. Discusiones y estudios recientes

    Directory of Open Access Journals (Sweden)

    Patricia Arias

    2013-01-01

    Full Text Available Con base en la revisión de algunos estudios recientes que se han llevado a cabo en diver- sas comunidades rurales de las nuevas regiones migratorias, en este artículo se revisan, de manera crítica, dos interpretaciones de los estudios sobre la familia rural: la economía campesina como unidad de producción-consumo y el ciclo de desarrollo de la unidad doméstica. En las condiciones actuales la migración, interna e internacional, desem- peña un papel decisivo en las comunidades rurales. Muchos estudios han constatado la voluntad de las mujeres de salir de los grupos domésticos y sumarse a los flujos migra- torios por motivos particulares, por situaciones y demandas específicas de ellas; su salida ha contribuido al resquebrajamiento de los sistemas tradicionales de organización y re- producción de la familia campesina. Las feministas, y más tarde los estudios con la perspectiva de género, criticaron la visión de que las familias rurales constituían unidades de producción-consumo donde las decisiones correspondían a un modelo de estrategias familiares de sobrevivencia y reproducción (Hondagneu-Sotelo, 2007; Wolf, 1990. Ese modelo privilegiaba la homo- geneidad, la colectividad, la solidaridad y el consenso, es decir, suponía que en los hogares no había conflictos ni tensiones a la hora de tomar decisiones que a todos com- prometían (Ariza, 2007. La familia era una “unidad económica moral” que se susten- taba en los principios de “reciprocidad, consenso y altruismo” (Grasmuck y Pessar, 1991. Los estudios desde el enfoque de género señalaron que en las familias había relacio- nes de poder basadas en una distribución jerárquica y desigual de los derechos, recursos y autoridad que afectaban especialmente a las mujeres (Ariza, 2007; González Montes, 2002; Hondagneu-Sotelo, 2007; Wolf, 1990. Las críticas alcanzaron a los estudios migratorios: la migración no era un fenómeno exclusivamente de los hombres, las mi

  11. Apoyo a Estudios Geodinamicos con GPS en Guatemala

    Science.gov (United States)

    Robles, V. R.

    2013-05-01

    El Instituto Geografico Nacional de Guatemala implemento 17 estaciones GNSS en el año 2009, como un proyecto de credito mixto de donacion de equipamiento del Gobierno de Suiza, el cual, este equipamiento de estaciones CORS GNSS es un sistema de recepción y transmisión de datos crudos GPS RInex que utiliza la tecnologia Spider Web de Leica, asi mismo este sistema esta sirviendo para el espablecimiento de un marco geodesico nacional de coordenadas geodesicas oficiales, el cual se calculan u obtienen las velocidades en tiempos temporales programados de las 17 Estaciones CORS. La infraestructura del marco geodesico de Guatemala esta sirviendo de base para las aplicaciones de estudios geodinamicos como el monitoreo de del desplazamiento de las placas tectonicas por medio de un estudio que se inicio en el año de 1999, llamado medicion con GPS el sistema de Fallas de los rios Polochic Motagua de Guatemala, tambien para un estudio que se implemento para deformación de corteza terrestre local en un Volcan Activo de Guatemala llamado Pacaya. Para el estudio de medicion con GPS en el sistema de falla de los Rios del polochic Motagua se implementaron 16 puntos para medir con GPS de dos frecuencias en el año de 1999, el cual, tres puntos son estaciones geodesicas CORS IGS llamados GUAT, ELEN y HUEH, despues en el año de 2003 se hizo otra medicion en un total de 20 puntos, que permitió calcular las velocidades de desplazamieinto de los puntos en mención, usando como referencia el modelo NUVEL 1A de DeMets de la placa de Norteamerica. Este estudio fue en cooperación internacional por la universidad de Nice de Francia y el IGNde Francia. Para el estudio del monitoreo con GPS del volcan activo de Guatemala, se implementaron cuatro puntos al rededor del volcan, el cual, se realizan cuatro mediciones al año, que permiten determinar axialmente la distancias entre los puntos, y rebisar estadisticamente cual es el comportamiento de las distancias en funcion del tiempo, si

  12. Individuos centrales como protagonistas del proceso de innovación: un estudio con blogs españoles relacionados con alimentación = Central individuals as leading actors in the innovation process: a case study with Spanish food-related weblogs

    Directory of Open Access Journals (Sweden)

    César Sahelices Pinto

    2014-06-01

    Full Text Available En el momento actual, el sector agroalimentario español atraviesa una de sus etapas más inciertas. Mientras el consumo extradoméstico se desploma, el doméstico se ralentiza por la búsqueda de fórmulas más racionales que resulten en un menor gasto. Las exportaciones, la válvula de escape, crecen a ritmos nunca antes vistos abriéndose paso en escenarios internacionales especialmente regulados. Como consecuencia de este entorno, el esfuerzo dirigido hacia la innovación se convierte en ineludible para las compañías del sector. Ante este hecho, el mismo consumidor se perfila como un recurso muy importante para la obtención de información acerca de nuevas ideas y necesidades no satisfechas que, gracias a Internet y a la Web 2.0, se encuentra ahora más que nunca al alcance de la empresa. Este trabajo de investigación aborda el estudio de una de estas redes sociales online, basado en el examen del comportamiento innovador de los individuos que las forman y en la identificación de aquéllos que socialmente destacan sobre los demás. El documento finaliza con una reflexión y justificación globales e indica además ciertos aspectos de utilidad para el mundo empresarial y sendas líneas de investigación futura.The Spanish food sector experiences at the present an especially uncertain conjunction. Whereas the extra-domestic consumption collapses, the domestic consumption slows down. Moreover, consumers look for more rational options at lower costs. In contrast, exports grow at really high rates in particularly regulated international scenarios. As a result of this scene, innovation efforts are unavoidable for food companies. In view of this, the very consumer, who is now easily reachable on the Internet and Web 2.0, represents a valuable resource for obtaining information about new ideas and uncovered needs. This research paper, working with one of these online social networks, presents an examination of the innovative behavior of

  13. Influence of available resources on medical practitioners' decision-making process and practice: study of a reference hospital emergency department Influencia de los recursos disponibles en la práctica clínica: estudio en una unidad de urgencias de un hospital de referencia

    Directory of Open Access Journals (Sweden)

    Juan Viñas Salas

    2007-04-01

    Full Text Available Objective: To evaluate variations in the observation period in the emergency department (ED in response to bed availability. Methods: A quasi-experimental pre-test post-test study without a control group was conducted in the ED observation ward over 2 1-month periods. During this time the only variable that changed was the number of beds available, which decreased from 20 (pre-test period to 16 (post-test period. Results: The ED attended 7,725 patients: 3,706 patients in pre-test period, 335 of whom were admitted to the observation ward, with an average length of stay of 1,105.4 minutes per patient, and 4,019 patients in post-test period, 570 of whom were admitted to the observation ward, with an average length of stay of 686.1 minutes per patient (p Objetivo: Evaluar la variación del indicador "periodo de tiempo de observación" dedicado a cada paciente, en respuesta a una reducción del número de camas disponibles. Métodos: Se realizó un estudio «quasiexperimental preprueba postprueba» sin grupo control en una unidad de urgencias durante 2 períodos de un mes. En este tiempo el único cambio fue el número de camas disponibles, que disminuyeron de 20 (período A a 16 (período B. Resultados: El servicio de urgencias atendió a 7.725 pacientes: 3.706 pacientes en el período A, 335 de los cuales fueron admitidos en la unidad de observación con una estancia media de 1.105,4 minutos por paciente; y 4.019 pacientes en el período B, 570 de los cuales fueron admitidos en la unidad de observación con una estancia media de 686,1 minutos por paciente (p < 0,001. No hubo variaciones en la mortalidad, readmisiones o quejas. Conclusiones: Una disminución en el número de camas disponibles para observación comporta una reducción en el tiempo de estancia de los pacientes.

  14. Estudio de la legislación ambiental en licencias de dragados de puertos en Brasil y España. Estudio de caso

    OpenAIRE

    VIÑES BERNARDO, MARGARITA

    2011-01-01

    Este TFC consistirá en la realización de un estudio de la legislación ambiental existente en relación al dragado de puertos en Brasil y en España, en el análisis de los procesos de licenciamiento y de la metodología de la monitorización del dragado aplicada en un estudio de caso. Viñes Bernardo, M. (2011). Estudio de la legislación ambiental en licencias de dragados de puertos en Brasil y España. Estudio de caso. http://hdl.handle.net/10251/10344. Archivo delegado

  15. Homicidas juveniles en Bogotá, estudio de grupos focales

    Directory of Open Access Journals (Sweden)

    Franklin Escobar Córdoba

    2015-07-01

    Materiales y Métodos. Estudio cualitativo mediante técnica de grupos focales. Resultados. Se encontró como el factor de riesgo más implicado la disponibilidad y uso de armas. Otros  factores de riesgo determinantes de la conducta delincuencial afectan la disposición del joven homicida para tener un comportamiento criminal y las estrategias de control que son elegidas por el individuo, dichos factores son apreciados de maneras distintas por los jóvenes homicidas y los no homicidas Conclusión. Este estudio arroja información clave que puede ser utilizada en el diseño e implementación de estrategias para enfrentar el homicidio juvenil como problema de salud pública.

  16. La actuación y los Estudios Literarios

    Directory of Open Access Journals (Sweden)

    Karina Mauro

    2011-05-01

    de los estudios literarios en la investigación sobre la Actuación a partir del análisis de los conceptos de representación y mimesis aristotélica y de los problemas que acarrea la aplicación de estos conceptos en el análisis de la acción del actor en escena.

  17. Estudio hidrogeológico del valle de Mala

    OpenAIRE

    Ministerio de Agricultura. Dirección General de Aguas, Suelos e Irrigaciones

    1980-01-01

    Determina el recurso hídrico subterráneo del valle del río Mala, mediante el análisis e investigación de las características hidrogeológicas, en base a las informaciones de diferentes etapas del estudio; para de esta manera, racionalizar mejor su uso, dando cumplimiento así a lo dispuesto por la Ley General de Aguas.

  18. Generalidades de los estudios de casos y controles

    Directory of Open Access Journals (Sweden)

    Alejandro González Garay

    2018-01-01

    Full Text Available ANTECEDENTES: los estudios de casos y controles son de utilidad cuando se buscan factores de riesgo para enfermedades poco comunes o que tienen un periodo de latencia prologado. OBJETIVO: identificar las principales características del estudio de casos y controles (Ca-Co para favorecer la disminución de sesgos durante su evaluación o conducción. DESCRIPCIÓN: este diseño se caracteriza por la selección de sujetos con o sin el evento de interés. Éstos se comparan para identificar los factores de riesgo que favorecieron la presencia de este evento. La finalidad de este tipo de estudios es la de inferir una relación causal expresada mediante razón de momios y los intervalos de confianza al 95%. CONCLUSIONES: el diseño de Ca-CO es relativamente fácil y rápido de realizar. Sin embargo, es susceptible a múltiples fuentes de sesgo, las cuales deben de ser minimizadas para obtener mayor confiabilidad de las inferencias obtenidas.

  19. Estudios marxistas de la cultura y los medios

    Directory of Open Access Journals (Sweden)

    Roy Alfaro Vargas

    2016-05-01

    Full Text Available Este artículo introduce los Estudios Marxistas de la Cultura y los Medios (EMCM a Latinoamérica y expone los principales fundamentos teóricos y epistemológicos de los estudios marxistas de la cultura y los medios. Se analiza la relación entre la teoría de la auto-organización, la dialéctica y la teoría crítica, alrededor del estudio de la Web 2.0 y de los sitios de redes sociales. Además, se explica el rol de la teoría marxiana del valor dentro de los EMCM, como medio para establecer la crítica del trabajo digital alienado realizado por el usuario de los sitios de redes sociales. En este sentido, se expone el concepto de Web 3.0 como medio para la superación dialéctica de la Web 2.0, en cuanto proceso de participación democrática, basada en la construcción de medios alternativos.

  20. Direct identification of microorganisms from positive blood cultures using the lysis-filtration technique and matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): a multicentre study.

    Science.gov (United States)

    Farina, Claudio; Arena, Fabio; Casprini, Patrizia; Cichero, Paola; Clementi, Massimo; Cosentino, Marina; Degl'Innocenti, Roberto; Giani, Tommaso; Luzzaro, Francesco; Mattei, Romano; Mauri, Carola; Nardone, Maria; Rossolini, Gian Maria; Serna Ortega, Paula Andrea; Vailati, Francesca

    2015-04-01

    Microbial identification from blood cultures is essential to institute optimal antibiotic therapy and improve survival possibilities. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been successfully applied to identify bacteria and yeasts from positive blood cultures broths. The aim of this multicentre study was to evaluate the reliability of the lysis-filtration technique associated with MALDI-TOF MS to directly identify microorganisms from 765 positive blood cultures collected in six Italian hospitals. Overall, 675/765 (78.1%) blood isolates were correctly identified at the species level, with significant differences between Gram-negative and Gram-positive bacteria (92.6%, and 69.8%, respectively). Some difficulties arise in identifying Streptococcus pneumoniae, Staphylococcus aureus, yeasts and anaerobes. The lysis-filtration protocol is a suitable procedure in terms of performance in identifying microorganisms, but it is quite expensive and technically time-consuming since the time of filtration is not regular for all the samples. The application of the MALDI-TOF MS technique to the direct microbial identification from positive blood cultures is a very promising approach, even if more experience must be gained to minimize errors and costs.

  1. Estudio y aplicación de diferentes metodologías de cálculo para el conocimiento gradual del proceso constructivo y comportamiento mecánico de una arquería de la Mezquita de Córdoba = Study and application of different calculation methodologies for the gradual knowledge of the construction process and mechanical behavior of an arcade of the Mosque of Cordoba

    Directory of Open Access Journals (Sweden)

    María Escalada Marco-Gardoqui

    2017-12-01

    Full Text Available La mecánica de las estructuras de fábrica ha sido durante mucho tiempo de alguna manera abandonada en la enseñanza universitaria durante la licenciatura de arquitectura, en comparación con otros campos del conocimiento, como el hormigón armado, el acero y la madera. Una parte importante de nuestro patrimonio arquitectónico se erigió empleando estas técnicas ampliamente difundidas antaño, pero ahora olvidadas tanto en la práctica constructiva como en el marco teórico de la universidad, más centrado en tecnologías contemporáneas e innovadoras. Los avances del software computacional como son los modelos macro/micro de elementos finitos nos permiten realizar un análisis extremadamente complejo de estructuras de mampostería, pero paradójicamente, al mismo tiempo, se distancian de las limitaciones y la forma en que los antiguos concibieron y entendieron la arquitectura. Los maestros canteros fueron capaces de construir perfectamente sin la ayuda de tales pruebas precisas sobre los materiales. Por lo tanto, la mentalidad que hay que adoptar a la hora de abordar la enseñanza del comportamiento de las estructuras de mampostería es aquella en la que se estimule al alumnado a apropiarse del conocimiento no solo de la geometría, sino también y principalmente de las fases del proceso constructivo y de las técnicas acordes al período histórico. Solo una vez elaboradas ciertas hipótesis sobre ellas, se deberá comenzar con el cálculo gráfico-numérico. El objetivo del presente trabajo es exponer una metodología de enseñanza basada en programas de optimización y ejemplificada con el estudio de un caso concreto: el análisis de las arquerías de la Mezquita de Córdoba y de los posibles sistemas de contrarresto provisionales de empujes mediante tirantes y apeos, buscando la rentabilidad material de los mismos. Se plantea un recorrido progresivo, con un gradiente de conocimiento, a través de un estudio gráfico-numérico que engloba

  2. Comprehensive Cross-Clade Characterization of Antibody-Mediated Recognition, Complement-Mediated Lysis, and Cell-Mediated Cytotoxicity of HIV-1 Envelope-Specific Antibodies toward Eradication of the HIV-1 Reservoir.

    Science.gov (United States)

    Mujib, Shariq; Liu, Jun; Rahman, A K M Nur-Ur; Schwartz, Jordan A; Bonner, Phil; Yue, Feng Yun; Ostrowski, Mario A

    2017-08-15

    Immunotherapy with passive administration of broadly neutralizing HIV-1 envelope-specific antibodies (bnAbs) in the setting of established infection in vivo has yielded mixed results. The contribution of different antibodies toward the direct elimination of infected cells is poorly understood. In this study, we determined the ability of 12 well-characterized anti-HIV-1 neutralizing antibodies to recognize and eliminate primary CD4 T cells infected with HIV-1 belonging to clades A, B, C, and D, via antibody-dependent complement-mediated lysis (ADCML) and antibody-dependent cell-mediated cytotoxicity (ADCC), in vitro We further tested unique combinations of these antibodies to determine the optimal antibody cocktails to be tested in future clinical trials. We report that antibody binding to infected CD4 T cells is highly variable and correlates with ADCML and ADCC processes. Particularly, antibodies targeting the envelope glycan shield (2G12) and V1/V2 site (PG9, PG16, and PGT145) are best at recognizing HIV-1-infected CD4 T cells. However, only PG9 and PG16 and their combinations with other bnAbs sufficiently induced the elimination of HIV-1-infected CD4 T cells by ADCML, ADCC, or both. Notably, CD4 binding site antibodies VRC01, 3BNC117, and NIH45-46 G54W did not exhibit recognition of infected cells and were unable to induce their killing. Future trials geared toward the development of a cure for HIV/AIDS should incorporate V1/V2 antibodies for maximal clearance of infected cells. With the use of only primary immune cells, we conducted a comprehensive cross-clade physiological analysis to aid the direction of antibodies as therapeutics toward the development of a cure for HIV/AIDS. IMPORTANCE Several antibodies capable of neutralizing the majority of circulating HIV-1 strains have been identified to date and have been shown to prevent infection in animal models. However, the use of combinations of such broadly neutralizing antibodies (bnAbs) for the treatment and

  3. El problema del abandono de los estudios universitarios. [The dropout problem in university study

    Directory of Open Access Journals (Sweden)

    Lidia Cabrera

    2006-07-01

    Full Text Available This article focuses on the dropout of university students, which in some cases can be as high as 50%. This study presents and analyses the dropout rates that are found in several Spanish universities. Using the results of national and international studies as background and the findings from our own investigation, we present a theoretical framework that identifies the definition of dropouts, the causes, the explanatory models and solutions used to understand this issue. The article concludes by presenting a critical perspective of university learning in relation to the social role that universities represent, and addresses the actual processes of change, particularly in light of the European convergence. En este artículo se analiza el fenómeno problemático del abandono de los estudios universitarios, que alcanza hasta el 50% en algunas titulaciones. Para ello, partimos de la presentación y análisis de los porcentajes de abandono de distintas universidades españolas. Posteriormente, al amparo de distintos estudios nacionales e internacionales, y nuestros resultados de investigación, elaboramos un perfil teórico que contempla: definición, causas, modelos explicativos y soluciones. Finalmente, ofrecemos una perspectiva crítica de la enseñanza universitaria, en relación al papel social que esta representa, y en relación a los actuales procesos de cambio, sobre todo el proceso de convergencia europea.

  4. The real value of the customer in the process of crm and accounting: a case study in a retail supermarket El valor real del cliente en el proceso de crm y la contabilidad: un estudio de caso en empresa del sector minorista supermercadista O valor real do cliente no processo de crm e a contabilidade: um estudo de caso em empresa do setor de varejo supermercadista

    Directory of Open Access Journals (Sweden)

    Tatiane da Silva Ferreira Vichroski

    2010-12-01

    Full Text Available As important as the gathering of transactional data and types and patterns of the customers is today, assessment of customer value, as precisely as possible, is critical in order to analyze and understand each of them in today's competitive marketplace. Thus, a way is proposed to assess the real value of the customer (LTV - Life Time Value for a retail supermarket based on their transactional and financial history as well as customer interactions with the organization. An exploratory case study was conducted in a supermarket to understand how LTV may be used in decision making in the process of relationship management with the customer (CRM - Customer Relationship Management. Results disclosed that the company with knowledge of the real value of its portfolio of customers can treat each customer segment on a more distinct personalized basis. This facilitates allocation of investments for the strategies of the relationship avoiding errors and helping the business to grow. Finally, the importance of accounting information generated by this process is emphasized as a modest academic contribution to the progress of studies on LTV, a subject widely discussed in international literature.En el mercado competitivo actual, tan importante cuanto reunir datos de transacciones, tipos y modelos de clientes es estimar lo más precisamente posible el valor del cliente, como forma de analizar y entender cada consumidor. En esa perspectiva, este trabajo propone una forma de estimar el valor real del cliente (LTV - Life Time Value del comercio minorista supermercadista con base en su historia de transacciones y financiera, bien como en sus interacciones con la organización. Para tanto, fue realizado un estudio exploratorio de caso único en empresa del ramo supermercadista, con el propósito de conocer como el LTV puede ser empleado al tomarse decisiones en el proceso de gerenciar las relaciones con el cliente (CRM - Customer Relationship Management. Como

  5. ISO 14006. Experiencias previas de estudios de arquitectura que han adoptado el estándar de ecodiseño UNE 150301:2003

    Directory of Open Access Journals (Sweden)

    Arana-Landín, G.

    2012-09-01

    Full Text Available This paper aims to analyze the pioneer UNE 150301 standard, as well as its adoption process and its practical results in the sector of architecture studios. First, the structure, the aim and the scope of norm UNE 150301 have been analysed. Second, the standard's implementation has been examined, concluding that 73% of the companies that have obtained the certificate are architecture studios. A case study has therefore been carried out with the participation of five architecture studios pioneers. These experiences have let us know the main aspects and difficulties of the process. In conclusion, the adoption of the standard UNE 150301 can be a helpful tool in order to reduce the environmental impact of the products and obtain some competitive advantages such as cost reduction, improvement in energy efficiency of the product and a better adaptation to acts and regulations.

    En este artículo se analiza la experiencia de implantación de la norma UNE 150301 de ecodiseño en el sector de los estudios de arquitectura. Tras el examen de la estructura de norma UNE 150301, de sus objetivos y de su alcance, se analiza su difusión, destacándose que el 73% de las empresas certificadas en España son estudios de arquitectura. A continuación se estudia el proceso de implantación real, mediante el estudio de caso que lo analiza en cinco estudios de arquitectura. El estudio realizado permite conocer de primera mano las principales claves y dificultades del proceso de implantación del estándar, así como los resultados obtenidos, entre los que destaca una reducción del impacto ambiental. Se constata, en suma, que la norma UNE 150301 es una herramienta que puede proporcionar ventajas competitivas interesantes a las empresas del sector de los estudios de arquitectura.

  6. Estudios pioneros en torno al origen del lenguaje natural

    Directory of Open Access Journals (Sweden)

    Martínez Contreras, Jorge

    2011-02-01

    Full Text Available It has being an ancient desire to ask apes what their natural lives are. De la Mettrie was the first to propose, in the 18th C., that the sign language of deaf adults could be used with them since they do not speak. We enhance here some of the pioneering projects of the two strategies for this endeavor: the use of ASL and the utilization of lexigrams and computers. Besides the ancient communicative quest with them, the evolutionist’s perspective has seen in these studies a way to find out how the natural language (NL emerged in hominids. If it is clear that apes do no possess totally the NL, the linguistic turn in primatology has left way, as in philosophy, to more complex field and laboratory cognitive studies of less anthropocentric nature.

    Tratar de hablar con los simios y preguntarles cosas sobre su vida natural es un viejo deseo. De la Mettrie propone, en el s. XVIII, que se les enseñe el lenguaje de los sordos en vista de que no pueden hablar. Resaltamos aquí algunos estudios pioneros en relación con las dos estrategias llevadas a cabo para tratar de hablar con los simios: el uso del lenguaje americano de sordos (ASL y el de los lexigramas y computadoras. Además del viejo interés interrogativo humano hacia ellos, la perspectiva evolucionista ha visto en estos estudios un medio de investigar cómo surgió el lenguaje natural (LN en homínidos. Si queda claro que los simios no poseen totalmente el LN, el giro lingüistico en primatología ha cedido, como en filosofía, el paso a estudios cognitivos más complejos, de campo y de laboratorio, de orientación menos antropomórfica.

  7. The comparative study of pressing and extrusion like processes of construction ceramic products in the Metropolitan Area of Cucuta; Estudio comparativo de las tecnicas de extrusion y prensado como procesos de conformado de productos ceramicos de construccion en el Area Metropolitana de Cucuta

    Energy Technology Data Exchange (ETDEWEB)

    Gelves, J. F.; Monroy, R.; Sanchez, J.; Ramirez, R. P.

    2013-02-01

    The present work studies the principal variables of control in the manufacturing process of construction pieces of the Metropolitan Area of San Jose de Cucuta by extrusion and pressing techniques for its forming. The investigation was taken out using clayey samples of the two principal geological formations of the region where the raw material is taken for processing at an industrial level. The clayey samples milling was made by dry means as well as by moisture means and its particle size was measured. Subsequently the forming process was taken over by using an hydraulic press and extruder with vacuum system , both equipment s at laboratory scale, the pieces shaped were dry and firing between 980 degree centigrade and 1180 degree centigrade at the end of the process the tests were made to determine water absorption, contraction and mass loss at the pieces firing. The study results left to see that the extrusion technique allowed a faster vitrification for the region's clay in comparing with the pressing technique, the contractions of drying and firing are less marked on the pressing techniques with standard deviations much lower than in extrusion. (Author) 13 refs.

  8. Instituciones educativas sustentables en Colombia caso de estudio colegio Rochester

    OpenAIRE

    García Gonzalez, Verónica; Bonilla Ruiz, Juliana; Cadena Sanchez, Laura Andrea

    2015-01-01

    En el proyecto en curso se pretende explicar cuáles son las condiciones y requisitos para implementar la cultura de organizaciones verdes en las instituciones educativas de nuestro país. Partiendo desde la transformación del colegio Rochester en un green school, teniendo en cuenta los requerimientos que exige el sistema de certificación LEED “Leadership Energy & Enviromental Desing” y los procesos administrativos que este involucra. Este estudio se llevara a cabo a través de una metodología q...

  9. Estudio de los Tweens y las Redes Sociales, Tacna, 2016.

    OpenAIRE

    Mamani Mamani, Deysi Gaby; Gonzalo Jara, Gustavo Richard; Yufra Tozo, Wilfredo Renato

    2016-01-01

    La tesis propuesta en las siguientes páginas: “Estudio de los Tweens y las Redes Sociales, Tacna, 2016”, busca poner en evidencia las preferencias, razones de uso, y uso, de las redes sociales por parte del grupo etario conformado por los tweens o pre adolescentes (Niños entre 8 y 12 años). La investigación presentada es de carácter descriptivo, de diseño no experimental y transversal, y se llevó a cabo aplicando un cuestionario (Asistido), conformado por 11 preguntas, a 146 niños entre la...

  10. Estudio del abandono empleando un modelo de riesgos proporcionales

    OpenAIRE

    Gonzalez, Mariana Verónica

    2017-01-01

    En este trabajo se aplica  un modelo de riesgo semi-paramétrico para identificar algunas variables que contribuyen a explicar el fenómeno del abandono estudiantil en una unidad académica de la Universidad Nacional de Córdoba (UNC), Argentina. La inactividad académica prolongada se toma como proxy de la variable dependiente. Los resultados muestran que el promedio del secundario aparece como importante predictor de la deserción. Además, se detectó que los estudiantes que costean sus estudios c...

  11. Cuidadores de Cartagena y su contexto de cuidado: estudio comparativo

    OpenAIRE

    Montalvo-Prieto, Amparo; Flórez-Torres, Inna Elida

    2009-01-01

    Objetivo: comparar las características sociodemográficas y de relación de cuidado de cuidadores de niños y adultos con enfermedades crónicas discapacitantes de Cartagena, Colombia. Método: estudio descriptivo, cuantitativo, de corte transversal. Se realizó un muestreo no probabilístico, por conveniencia, entre el 2006 y el 2007. Participaron 300 cuidadores: 98 de niños y 202 de adultos con enfermedades crónicas. Se utilizó la encuesta de caracterización de los cuidadores. Para describir las a...

  12. Aborto y Parlamento: un estudio sobre Brasil, Uruguay y Argentina

    OpenAIRE

    Rocha,Maria Isabel Baltar da; Rostagnol,Susana; Gutiérrez,María Alicia

    2009-01-01

    El presente trabajo desarrolla un estudio comparativo entre Brasil, Argentina y Uruguay sobre las legislaciones y las acciones de diversos actores en relación a la despenalización y/o legalización del aborto. El tema se instala en la agenda pública en los tres países a partir del proceso de democratización iniciado en la década de los 80 produciéndose diversas intervenciones tanto del movimiento de mujeres, como de las corporaciones médicas y otros actores sociales en el transcurso de la déca...

  13. Estudios de impacto ambiental en viaductos de gran longitud

    OpenAIRE

    Moya Matute, David

    2009-01-01

    El contenido de esta tesina forma parte de un proyecto concedido por el Ministerio de Educación y Ciencia en el año 2006 y coordinado entre el Departamento de Ingeniería de la Construcción de la UPC y el Departamento de Ingeniería Civil de la UCLM. El objeto de dicho proyecto de investigación es el estudio de la optimización de viaductos de gran longitud desde distintas vertientes, como son: estructural, plazo de ejecución, impacto ambiental, seguridad y salud, costes globales, et...

  14. Análisis y estudio del microcemento

    OpenAIRE

    PENADÉS SANZ, JONATAN

    2015-01-01

    [es] El presente Trabajo Final de Grado trata sobre el microcemento, que es un revestimiento continuo multicapa a base de resinas poliméricas, áridos y elementos cementosos. En este trabajo se analizan sus propiedades y su forma de aplicación, también se mencionan algunos consejos a tener en cuenta para su correcto empleo, y se estudian tanto sus ventajas e inconvenientes como sus rendimientos y costes. Además del estudio teórico se desarrolla una aplicación práctica mediante la realización d...

  15. Estudio de viabilidad de una central de restaurantes

    OpenAIRE

    Sáez Lanaspa, Idoya

    2015-01-01

    Mediante este estudio de viabilidad hemos analizado un nuevo campo dentro de la gastronomía y veremos su impacto en el mercado. Queremos presentar un nuevo concepto de restaurante, fuera de lo tradicional, de comida rápida basado en el buen gusto, la comida saludable e inspirado en una nueva idea de servicio más común en otros países como Estados Unidos. Instalado en un contenedor reciclado, reformado y adaptado con zona de comedor y cocina visible, creando un ambiente agrad...

  16. Estudio de factibilidad para un calzado personalizable femenino

    OpenAIRE

    Durán Pimienta, Oscar Adrián; Jaimes Santamaría, Juan Camilo

    2011-01-01

    El presente documento describe el proceso de investigación realizado por dos estudiantes de la Universidad EAFIT sobre la factibilidad de desarrollar un calzado personalizable femenino, en este proceso se llevaron a cabo diferentes estudios primarios con el fin de poseer una contextualización sobre la situación económica tanto del sector calzado, cuero y sus manufacturas, como del país -- Además se realizó una investigación sobre los diferentes procesos necesarios para la realizac...

  17. Aprender a dormir sin mam??: estudio de casos

    OpenAIRE

    ??lvarez Lozano, Marta

    2015-01-01

    Dado el caso de un ni??o con un h??bito incorrecto de sue??o, con sus correspondientes consecuencias, se lleva a cabo un estudio de casos que pretende modificar este h??bito a trav??s de un programa de intervenci??n basado en la reeducaci??n del sue??o mediante la modificaci??n de conducta. Para proceder a modificarla se ha trabajado con un m??todo de desensibilizaci??n sistem??tica, que a su vez, se ayuda de un sistema de econom??a de fichas para causar motivaci??n en el sujeto, y adem??s, d...

  18. Aproximaciones proteómicas para el estudio del envejecimiento

    OpenAIRE

    Fanjul Hevia, Víctor

    2014-01-01

    La población mundial está envejeciendo progresivamente y la edad es el factor principal de riesgo de enfermedad cardiovascular (ECV), que a su vez es el principal problema de salud en países desarrollados. Aún no se conocen en profundidad los mecanismos moleculares que provocan el envejecimiento ni aquéllos que originan la ECV, y su estudio podría conducir hacia el desarrollo de estrategias preventivas o de terapias que alarguen significativamente la vida y mejoren su calidad. La progeria ...

  19. Stuart Hall sobre “hacer estudios culturales”

    OpenAIRE

    Mato, Daniel; Universidad Nacional Tres de Febrero (Untref), Buenos Aires, Argentina

    2016-01-01

    Traducción de Emeshe Juhász MininbergEmeshe Juhász Mininberg es escritora e investigadora independiente. Doctora en Filosofía y Letras, Yale University, Estados Unidos. Traductora y editora de textos de crítica cultural y de artes plásticas. Ha publicado varios artículos sobre ciudadanía, cultura e identidad nacional en tiempos de globalización, que aparecen, entre otros, en Cultura, política y sociedad: perspectivas latinoamericanas (Clacso, 2005) y en el Diccionario de estudios culturales l...

  20. Estudio electrocinético de un suelo sometido

    OpenAIRE

    Hernandez-luis, Felipe; Ramirez, Carolina; Arbelo, Carmen

    2011-01-01

    En el presente trabajo se analizan algunos cambios producidos en las propiedades de un suelo sometido a impacto térmico. Para llevar a cabo este estudio se utiliza una técnica electrocinética basada en los mismos principios que la electrorremediación. El análisis se enfoca en la variación, durante el tratamiento, de la corriente de electrólisis y de la diferencia de potencial generada en el interior del suelo en una región cercana a la cámara de los electrodos...

  1. Estudio de la Esquizofrenia en el Núcleo Familiar

    OpenAIRE

    Núñez-Borja Castro, Luis Alberto

    2017-01-01

    La presente tesis desarrolla el Estudio de la Esquizofrenia en la familia a partir de tres casos de hogares que cuentan entre sus miembros con un paciente diagnosticado con Esquizofrenia y con el cual conviven día a día. Se evaluó los cambios que ocurren y están ocurriendo en el comportamiento en el seno de tres familias que viven con un paciente diagnosticado con este trastorno mental. Para llevar a cabo esta investigación se trabajó con una muestra de tres pacientes aquejados con este...

  2. Estado Actual y Retos Futuros en el Estudio del Burnout

    OpenAIRE

    Salanova Soria, Marisa; Llorens Gumbau, Susana

    2008-01-01

    El objetivo principal de este artículo es ofrecer una revisión de la actual investigación aplicada del síndrome de quemarse por el trabajo o burnout. Comenzamos con el concepto de burnout, su evolución desde los primeros estudios en ocupaciones de servicios, hasta la actualidad en donde la investigación ha demostrado la importancia de considerar otras ocupaciones, y ampliar las dimensiones clave del burnout. En segundo lugar, examinaremos cómo evaluar el burnout, en función de la pos...

  3. Modelos parentales en el contexto urbano: un estudio exploratorio

    OpenAIRE

    Martínez Licona, José Francisco; García Rodrigo, María Isabel; Collazo Martínez, Jonathan Carlos

    2017-01-01

    Resumen: El propósito de este trabajo es explorar las características que tienen las familias potosinas en cuanto a su cultura parental; es decir, explorar sus pautas de crianza, los diferentes vínculos de apego que se construyen y los patrones de éxito que se promueven para relacionarlos con sus características biográficas. El estudio se efectuó en la zona conurbada de San Luis Potosí (México), con la participación de 1008 familias de diferentes niveles culturales y se utilizó un instrumento...

  4. Estudio de los galactanos del alga roja pterocladiella Capillacea

    OpenAIRE

    Errea, María Inés

    2001-01-01

    Existen dos familias de polisacáridos sintetizados por algas rojas, los carragenanos y el ágar, que, debido a sus propiedades reológicas, que les permiten estabilizar soluciones o emulsiones acuosas, son utilizados en la industria farmacéutica y alimentaria de todo el mundo. Este Trabajo de Tesis comprende los tópicos que se indican a continuación: a) Estudio de la estructura de los galactanos que componen el sistema de polisacáridos de la agarofita Plerocladíella capillacea (Gelidiales, Geli...

  5. Efectividad de la maniobra de Sellick: un estudio con Glidescope

    OpenAIRE

    Muñoz Ávalos, Natalia; Robledo, María de la Flor

    2014-01-01

    La compresión cricoidea fue descrita en 1961 por Sellick como método para reducir el riesgo de broncoaspiración durante la fase de inducción de una anestesia general, formando parte de los pilares de la secuencia de intubación rápida. No obstante, cada vez son más los detractores que cuestionan la efectividad de esta maniobra a la luz de las últimas publicaciones . El presente estudio propone verificar la efectividad de la presión cricoidea en ocluir la luz esofágica mediante visión dir...

  6. Estudios sobre metales arqueológicos quemados

    OpenAIRE

    Montero Ruiz, Ignacio; Rovira Llorens, Salvador

    2002-01-01

    El registro arqueológico nos ofrece materiales metálicos que han sido sometidos a un proceso de quemado ya sea intencional, por su inclusión en ajuares funerarios con el rito de cremación, bien de manera accidental por fuegos o incendios. Se intentan observar e identificar las huellas que este proceso ha podido dejar en materiales metálicos no ferreos: unas veces visualmente reconocibles por la propia deformación del objeto y otras acudiendo a los estudios metalográficos para observar la estr...

  7. Traumatismo torácico. Estudio de un cuatrienio

    OpenAIRE

    Moya Rosa, Enrique J; Marrero Peláez, Niuris

    2002-01-01

    Se realizó un estudio descriptivo con los expedientes clínicos de 252 pacientes afectados por trauma de tórax, ingresados en la unidad de politrauma del Hospital Provincial Docente Manuel Ascunce Domenech de Camagüey, desde enero de 1996 a diciembre de1999. Analizamos las variables edad, sexo, causas, tipo de lesión, su relación con otras regiones lesionadas, así como el estado de los pacientes al egresar. Existió un predominio del sexo masculino con respecto al femenino; los grupos de edades...

  8. El abandono en las etapas iniciales de los estudios superiores

    OpenAIRE

    Norma Zandomeni; Sandra Canale; Andrea Pacifico; Fernanda Pagura

    2016-01-01

    En este artículo se presenta una investigación sobre abandono de los estudios superiores realizada en una universidad argentina. Con un abordaje metodológico cuanti-cualitativo, se analizan datos secundarios y se recupera la perspectiva de distintos actores mediante talleres, encuestas y entrevistas. Se aborda el abandono que se produce en el proceso del ingreso y el generado en los dos primeros años, tanto en su cuantificación como en los factores que operan en el mismo. En el primer caso el...

  9. Wear studies in the shearing process by means of irradiated tools; Etudes d'usure dans les operations de cisaillement, au moyen d'outils irradies; Issledovaniya problemy iznosa v protsesse skalyvaniya posredstvom obluchennykh instrumentov; Estudios de desgaste en las operaciones de cizallamiento, realizados con ayuda de herramientas irradiadas

    Energy Technology Data Exchange (ETDEWEB)

    Sata, Toshio; Abe, Kunio; Nakajima, Kiyoshi [Institute of Physical and Chemical Research, Komagome, Bunkyo-Ku, Tokyo (Japan)

    1962-01-15

    pastilles, tandis que la radioactivite des trous variait a peine. Cela montre que l'usure de la matrice peut etre mesuree d'apres la radioactivite des pastilles, et celle du poincon d'apres la radioactivite des trous. On a eu recours a cette methode pour suivre le processus d'usure du poincon et de la matrice en employant differents lubrifiants et differentes toles, ce qui a permis de faire les constatations suivantes: 1. Au debut du poinconnage, le taux d'usure du poincon et celui de la matrice sont tres eleves mais diminuent rapidement, pour devenir finalement stationnaires apres decoupage d'environ 4 a 500 pieces. 2. L'usure du poincon est d'environ 20 % plus forte que celle de la matrice. 3. Les lubrifiants contenant des additifs qui resistent aux pressions extremes, tels que le chlore, le phosphore ou le soufre, reduisent l'usure des outils, tandis que les huiles minerales raffinees n'apportent guere d'amelioration. 4. La plus forte usure des outils se produit lors du poinconnage des toles les plus dures. Cette usure est environ trois fois plus forte pour l'acier inoxydable et environ six fois plus forte pour l'acier riche en silicium que pour l'acier pauvre en carbone. (author) [Spanish] Se ha estudiado el desgaste de las herramientas utilizadas en el corte de laminas metalicas con punzones y matrices de acero rapido irradiados en un reactor. Se recortaron a punzon discos de 10 mm de diametro en laminas de acero pobre en carbono, acero inoxidable y acero al silicio de 0,5 mm de espesor en estado seco y lubricado. Despues del punzonamiento, se midio la radiactividad de los discos y de los orificios. Al reemplazar la matriz radiactiva por otra no irradiada, se detecto escasa actividad en los discos, mientras que la radiactividad de los orificios apenas sufrio variacion. Ello demuestra que el desgaste de la matriz y del punzon pueden medirse en funcion de la radiactividad de los discos y de los orificios, respectivamente. Con ayuda de este metodo, se estudio el desgaste

  10. Study of the quasi-two body processes including {sigma}(1385) production in {pi}{sup -}p and K{sup -} reactions at 4 GeV/c; Estudio de procesos cuasi-dos cuerpos con produccion de {sigma} (1385) en interacciones {pi}{sup -}p y K{sup p} A 4 GeV/c

    Energy Technology Data Exchange (ETDEWEB)

    Aguilar-Benitez, M; Salicio, J

    1981-07-01

    An analysis of (1385) production in reactions of the type 0{sup +} 1/z 4 +{yields}>-1{sup +} 3+/2 and 0{sup -} + 1/2+{yields} 0{sup -} + 3*/2 is presented. A determination of the {sigma}(1385) production parameters Is performed and the results are compared with the predictions from several models. A transversity amplitudes reconstruction describing the processes {pi} {sup p} ->K(890) {sigma}(1385) and K{sup -}p {yields} 3{yields}{phi}(1385), {zeta}{sup -}{sigma}(1385) is obtained in a model independent way. We observe dominance of unnatural partly exchange in the production mechanisms. Exchanges of exotic quantum numbers are established by the study of {pi}p {yields} K{sup 0}+ {sigma}(1385)s and K{sup -} p{yields}>{pi}+{sigma}(1385){+-} processes. Additive quark model predictions are reasonable agreement with the experimental data. (Author)

  11. Una revisión general a los hábitos y técnicas de estudio en el ámbito universitario

    Directory of Open Access Journals (Sweden)

    María Fernanda Enríquez Villota

    2015-01-01

    Full Text Available El presente artículo pretende desarrollar una revisión teórica a partir de la selección, organización, sistematización y análisis de un grupo de textos y documentos electrónicos referidos a los hábitos y técnicas de estudio. Todo ello con la intención de responder los siguientes interrogantes: ¿Qué son los hábitos de estudio y cómo afectan el aprendizaje de los universitarios?, ¿qué son las técnicas de estudio?, ¿cuáles son las técnicas de estudio más empleadas por los estudiantes universitarios en la actualidad? Se concluyó que el estudio de este tema tiene una importante vigencia, debido a la necesidad práctica de evaluar, comprender y mejorar estos factores en los estudiantes de todos los niveles escolares, y en especial en los que cursan estudios superiores, a fin de optimizar los procesos de enseñanza y aprendizaje desarrollados en los contextos escolares. Abstract This article aims to develop a theoretical review on the theme ‘Habits and Study Skills’ with the purpose on showing the selection, organization, systematization and analysis of a group of texts and electronic documents related to the subject. The results seek to answer questions such as: Which are the study habits and how do they affect learning for university students? What are study skills? And which are some of the study techniques employed by most college students today? Concluding that the study is very relevant because of the practical need to assess, understand and improve these factors in students of all school levels and especially in higher education, with the purpose of optimizing the teaching and learning processes developed in school contexts.

  12. Estudio sobre el estado nutricional, calidad de vida, y capacidad funcional en pacientes con fibromialgia. Estudio ENCAVI

    OpenAIRE

    Arranz, Laura Isabel

    2012-01-01

    [spa] La fibromialgia es una enfermedad reumática, clasificada con el código OMS CIE-10- M79.7 (versión 2007), de carácter crónico que causa dolor muscular generalizado, rigidez, fatiga, alteraciones del sueño y trastornos cognitivos entre otros síntomas. La prevalencia en España está alrededor del 2.4%, aunque algunos estudios han situado esta cifra entorno al 4%, siendo las mujeres las que más la padecen, con gran diferencia respecto a los hombres. La fibromialgia, además, se presenta frecu...

  13. Contexto actual de los estudios preclínicos

    Directory of Open Access Journals (Sweden)

    Thelvia I. Ramos

    2016-09-01

    Full Text Available El desarrollo acelerado de la Industria Biofarmacéutica, y en particular de la producción de biológicos, ha permitido el incremento de la investigación clínica, teniendo en consideración la novedad de las sustancias que se desarrollan. El éxito de un producto solo se logra a través de la realización de los estudios clínicos controlados, lo cual significa que este ha sido validado a través de un sistema de gestión de calidad, principal determinante para que la molécula en estudio, pueda ser autorizada y comercializada posterior a la demostración de su seguridad y eficacia. Previo a la presentación de una solicitud para un nuevo producto ante las autoridades regulatorias, es necesario pasar por la etapa de investigación preclínica.

  14. Viviendo en Flatland. El estudio comparado del crimen violento

    Directory of Open Access Journals (Sweden)

    Luis David Ramírez de Garay

    2013-05-01

    Full Text Available Esta nota de investigación indaga sobre el notorio rezago que la criminología y la sociología del crimen tienen en la aplicación de la metodología comparada. A diferencia de la ciencia política y algunas áreas de la investigación sociológica, la metodología comparada no ocupa un lugar preponderante en la investigación empírica del crimen. Sin embargo, existen algunos valiosos ejemplos sobre las ventajas del estudio comparado del crimen y del crimen violento. Por ello, incluyo una breve revisión del estado actual de dichos estudios y de sus inherentes limitaciones teóricas y metodológicas. Finalmente, concluyo la nota presentando mi propuesta para incorporar la metodología comparada en la agenda de investigación sobre las bases sociales del crimen y del crimen violento

  15. ESTUDIO CINEANTROPOMÉTRICO DEL JUGADOR DE TENIS ADOLESCENTE

    Directory of Open Access Journals (Sweden)

    Gema Torres Luque

    2006-01-01

    Full Text Available El propósito de este estudio fue aproximarse al perfil cineantropométrico de jugadores de tenis de edad adolescente. Para ello se seleccionaron 75 jugadores de tenis (47 varones y 26 mujeres a los cuales se les determinó el perfil antropométrico, composición corporal y somatotipo, mostrando a su vez las diferencias en cuanto al género. Todas las valoraciones se realizaron siguiendo las recomendaciones del Grupo Español de Cineantropometría, los tenistas fueron evaluados a la misma hora del día. Los resultados muestran diferencias significativamente mayores en los tenistas en peso, talla, perímetros bioestiloide y bicondíleo húmero, así como en diámetros en brazo contraído y perímetro del muslo y pierna. A su vez, las tenistas muestran un mayor porcentaje de grasa corporal y un menor porcentaje de masa ósea, no existiendo diferencias significativas respecto al porcentaje de masa muscular. El somatotipo marca una tendencia ecto mesomórfica para el género masculino, y una tendencia meso endomórfica para el género femenino. El estudio cineantropométrico del tenista adolescente permitirá una aproximación de referencia al perfil funcional del tenista y contribuir al máximo rendimiento deportivo.

  16. Estudio de inmunogenicidad para dos vacunas recombinantes contra hepatitis B

    Directory of Open Access Journals (Sweden)

    O. Juliao

    1991-12-01

    Full Text Available Este estudio compara la inmunogenicidad (seroconversión, seroprotección e Hiperrespuesta, producida por dos vacunas recombinantes contra la hepatitis B (Engerix-B de Bélgica y Cubana, en dos esquemas (012 y 016 meses, empleando los métodos de cuantificación para Anti-HBsAg (Abbott y Organón, los cuales fueron también comparados. En el estudio participaron 257 voluntarios,  divididos al azar en 4 grupos (dos vacunas, dos esquemas. Resultados: los dos métodos de Abbon y Organon, no presentan diferencias estadísticas significativas. La vacuna cubana muestra una mayor respuesta inmunogénica para dos dosis de vacuna y para el esquema 012. No hay diferencia entre los esquemas 012 y 016 y en el esquema 016 no se ven diferencias estadísticamente significativas con la vacuna Engerix-B. En esta Última el esquema 016 muestra mejores resultados que el 012.

  17. Litiasis renal: estudio y manejo endocrinológico

    Directory of Open Access Journals (Sweden)

    V. Gilberto González, Dr.

    2013-09-01

    Full Text Available La litiasis renal es causa de importante morbilidad y costo económico, afectando hasta el 15% de la población. Además, la litiasis renal puede ser expresión también de enfermedades extrarrenales, entre las cuales destaca riesgo aumentado de osteoporosis. Estudios nacionales muestran que las causas de litiasis renal en Chile son similares a las comunicadas internacionalmente, destacando la hipercalciuria idiopática como el principal factor de riesgo. A pesar de los avances en técnicas urológicas de remoción de cálculo, éstas no modifican la historia natural de la litiasis renal. Así, en los pacientes sin tratamiento médico, la recurrencia es la regla más que la excepción. En esta revisión se actualiza el estudio y manejo endocrinológico de la litiasis renal, para el cual la evidencia muestra que éste es eficaz y seguro en la prevención de recurrencia de litiasis renal y control de enfermedades asociadas, complementando así el manejo urológico de esta importante enfermedad.

  18. Preliminares al Estudio del Chancro y la Fusariosis del Cacao

    Directory of Open Access Journals (Sweden)

    Garcés O. Carlos

    1939-08-01

    Full Text Available El presente estudio es el fruto de año y medio de investigaciones continuas, durante las cuales el autor ha pretendido recoger el máximum de observaciones personales que puede obtenerse cuando, a más de la deficiencia técnica y de laboratorio que ocurre en quienes se inician en Patología Vegetal, se dispone de medios que requiere habilidad y conocimientos profundos por parte del observador y considerable campo de experimentación. No hay por este motivo creación de doctrinas sino simplemente exposición de observaciones experimentales, que pueden estar erradas por deficiencia técnica, pero que se han efectuado con gran pulcritud de conciencia. El autor agradece sinceramente q quienes con voluntad inmejorable se prestaron a suministrarle toda clase de datos relacionados con este estudio y rinde homenaje de cordial reconocimiento al doctor Ramón Mejía Franco, constante propulsor de sus esfuerzos y guía seguro en la mayor parte de las experimentaciones.

  19. LIDERAZGO MULTICULTURAL: ESTUDIO COMPARATIVO INDIA-MÉXICO

    Directory of Open Access Journals (Sweden)

    BERTA ERMILA MADRIGAL TORRES

    2017-01-01

    Full Text Available En este artículo se presenta un estudio de caso de una empresa donde colaboran directivos de dos culturas: México e India; se plantean preguntas de investigación como: ¿cuáles son los valores, actitudes y habilidades que ambas culturas esperan de sus líderes?, ¿cuáles son las habilidades del líder y sus estilos de liderazgo? y ¿cuál es la distancia y dimensión del poder de los líderes de ambas culturas? Se diseñó un instrumento con las cinco características de la descripción de la personalidad enumeradas por Goldberg (1990, la aproximación de estilo de liderazgo de Madrigal (2009, y dimensión de la incertidumbre y distancia del poder con la teoría de Northouse (2012. Para poder llevar a cabo este estudio se encuestaron a 102 directivos de cultura india y mexicana en diferentes aspectos relacionados con la percepción de liderazgo. Los resultados demuestran que tanto los mexicanos como los indios tienden a preferir un estilo de liderazgo democrático. Finalmente se presentan como hallazgos las diferencias existentes en el colectivismo institucional y en la orientación al futuro de ambas culturas.

  20. Multi-parameter flow cytometry as a process analytical technology (PAT) approach for the assessment of bacterial ghost production.

    Science.gov (United States)

    Langemann, Timo; Mayr, Ulrike Beate; Meitz, Andrea; Lubitz, Werner; Herwig, Christoph

    2016-01-01

    Flow cytometry (FCM) is a tool for the analysis of single-cell properties in a cell suspension. In this contribution, we present an improved FCM method for the assessment of E-lysis in Enterobacteriaceae. The result of the E-lysis process is empty bacterial envelopes-called bacterial ghosts (BGs)-that constitute potential products in the pharmaceutical field. BGs have reduced light scattering properties when compared with intact cells. In combination with viability information obtained from staining samples with the membrane potential-sensitive fluorescent dye bis-(1,3-dibutylarbituric acid) trimethine oxonol (DiBAC4(3)), the presented method allows to differentiate between populations of viable cells, dead cells, and BGs. Using a second fluorescent dye RH414 as a membrane marker, non-cellular background was excluded from the data which greatly improved the quality of the results. Using true volumetric absolute counting, the FCM data correlated well with cell count data obtained from colony-forming units (CFU) for viable populations. Applicability of the method to several Enterobacteriaceae (different Escherichia coli strains, Salmonella typhimurium, Shigella flexneri 2a) could be shown. The method was validated as a resilient process analytical technology (PAT) tool for the assessment of E-lysis and for particle counting during 20-l batch processes for the production of Escherichia coli Nissle 1917 BGs.

  1. Optimization and studies of the welding processes, automation of the sealing welding system and fracture mechanics in the vessels surveillance in nuclear power plants; Optimizacion y estudios de los procesos de soldadura, automatizacion del sistema de soldadura de sellado y mecanica de fractura en la vigilancia de vasijas en nucleoelectricas

    Energy Technology Data Exchange (ETDEWEB)

    Gama R, G.

    2011-07-01

    Inside this work the optimization of two welding systems is described, as well as the conclusion of a system for the qualification of containers sealing in the National Institute of Nuclear Research that have application in the surveillance programs of nuclear reactors vessels and the correspondent extension of the operation license. The test tubes Charpy are assay to evaluate the embrittlement grade, when obtaining the increment in the reference temperature and the decrease of the absorbed maximum energy, in the transition curve fragile-ductile of the material. After the test two test tube halves are obtained that should take advantage to follow the surveillance of the vessel and their possible operation extension, this is achieved by means of rebuilding (being obtained of a tested test tube two reconstituted test tubes). The welding system for the rebuilding of test tubes Charpy, was optimized when diminishing the union force at solder, achieving the elimination of the rejection for penetration lack for spill. For this work temperature measurements were carried out at different distances of the welding interface from 1 up to 12 mm, obtaining temperature profiles. With the maximum temperatures were obtained a graph and equation that represents the maximum temperature regarding the distance of the interface, giving as a result practical the elimination of other temperature measurements. The reconstituted test tubes were introduced inside pressurized containers with helium of ultra high purity to 1 pressure atmosphere. This process was carried out in the welding system for containers sealing, where an automatic process was implemented by means of an application developed in the program LabVIEW, reducing operation times and allowing the remote control of the process, the acquisition parameters as well as the generation of welding reports, avoiding with this the human error. (Author)

  2. Estudio epidemiológico de salud oral en adultos. Comunidad Valenciana, 2006

    OpenAIRE

    Eustaquio Raga, Mª Vicenta

    2008-01-01

    RESUMEN En las últimas dos décadas se han venido realizando estudios epidemiológicos de salud oral en adultos en las diferentes comunidades autónomas españolas. Así la ausencia de dicho estudio de ámbito autonómico valenciano justifica plenamente su realización. En la realización del presente estudio nos hemos propuesto como objetivo general el evaluar el estado de salud bucodental de la población adulta y mayor de la Comunidad Valenciana. Se ha diseñado un estudio transversal o de p...

  3. GC/MS in the study of deterioration process of historical monuments: characterization of the black crust formed in the Cathedral of Seville; GC/MS en el estudio del proceso de deterioro de monumentos historicos: caracterizacion de la costra negra presente en la Catedral de Sevilla

    Energy Technology Data Exchange (ETDEWEB)

    Reyes, J. [Programa de Corrosion del Golfo de Mexico. Universidad Autonoma de Campeche. Av. Agustin Melgar s/n, Campeche (Mexico); Hermosin, B.; Saiz J, C. [Instituto de Recursos Naturales y Agrobiologia de Sevilla. Avenida de la Reina Mercedes 10. Sevilla, Espana (Spain)]. e-mail: javreyes@uacam.mx

    2006-07-01

    Here, we present an interesting application of Gas Chromatography/Mass Spectrometry (GC/MS) technique which permitted to differentiate successfully between natural and anthropogenic compounds present in the black crust formed in the Cathedral of Seville. This building, considered as one of the most important historic and cultural European monuments suffers from intense blackening of its outside walls and sculptures due to the interaction with the urban environment. Fragment of black crusts were removed from the Cathedral walls and extraction process with organic solvents was carried out. The aliphatic, aromatic and polar fractions were separated and analyzed using GC/MS. A complex organic composition of the black crusts indicated the accumulative character of the deposition process of atmospheric particles as a function of the time. The presence of high molecular weight n-alkanes, n-fatty acids and natural molecular markers identified during the study were indicative of current biogenic contributions. It was also found an important group of petrogenic molecular markers related with diesel soot emissions from motor engines. The diesel soot and molecular markers were entrapped within the black crust mineral matrix and contribute to the observed blackening of the Seville Cathedral. (Author)

  4. Study of the thermo-mechanical behavior of medium carbon microalloyed steel during hot forming process using an artificial neural network; Estudio del comportamiento termo-mecanico de un acero microaleado de medio carbono durante un proceso de conformado en caliente usando una red neuronal artificial

    Energy Technology Data Exchange (ETDEWEB)

    Alcelay, I.; Pena, E.; Al Omar, A.

    2016-10-01

    The thermo-mechanical behavior of medium carbon microalloyed steel has been analyzed by an Artificial Neural Network (ANN). The flow curves for training the ANN have been obtained from the hot compression tests, carried out over a temperature range varying from 900 to 1150 degree centigrade and at different true strain rates ranging from 10{sup -}4 to 10 s{sup -}1. It has been found that the ANN model developed in this study is capable to predict accurately and efficiently the flow behavior of the studied steel and there is a good agreement between the experimental results and the ANN results. To analyze the formability of the studied steel, processing maps have been constructed on the basis of the Dynamic Materials Model (DMM), using the ANN values of the flow stress. The study of maps reveals the different domains of the flow behavior of the studied steel and shows the great similarity between the experimental results and the theoretical results, so the use of the ANN can constitute an interesting alternative for design and study of hot forming processes. (Author)

  5. Estudio del impacto ambiental de medicamentos de control especial en Bogotá, Colombia. Caso de estudio: Lorazepam

    Directory of Open Access Journals (Sweden)

    Ibeth Eileen López

    2016-01-01

    Full Text Available El aumento de la población y la cobertura de los sistemas de salud, así como el crecimiento del mercado farmacéutico, ha dado lugar a la presencia de una nueva generación de residuos peligrosos, que no habían sido considerados como tal. En Colombia, no se han realizado suficientes estudios relacionados con el impacto ambiental ocasionado por la disposición final de medicamentos, porque no existen cifras disponibles acerca de la cantidad depositada diariamente en rellenos sanitarios, destruida en plantas gestoras de residuos peligrosos o encontrada en plantas de tratamiento de aguas residuales. Se encontró en esta investigación que sólo en Bogotá, se destruyen en promedio anualmente 9.310.123 unidades farmacéuticas de productos de control especial, sin considerar las unidades desechadas a nivel doméstico. Para estudiar el impacto ambiental, se seleccionó el principio activo lorazepam, la información sobre la cantidad del medicamento consumida y destruida en plantas gestoras de residuos peligrosos en la ciudad de Bogotá, durante los años 2012 y 2013, fue aportada por el Fondo Nacional de Estupefacientes. Los datos fueron evaluados utilizando la matriz de Leopold, software especializado y ensayos de laboratorio complementarios. Este estudio pretende sensibilizar a fabricantes, prescriptores y pacientes sobre la responsabilidad ambiental en el uso de medicamentos de control especial.

  6. Instrumental set up for the study of water erosion processes to different scales in the upper Barranc de Carraixet water basin; Diseno instrumental para el estudio de los procesos de erosion hidrica a distintas escalas en la cuenca alta del Barranc del Carraixet

    Energy Technology Data Exchange (ETDEWEB)

    Rubio, J. L.; Pascual-Aguilar, J. A.; Andreu, V.

    2009-07-01

    As a result of the rainfall conversion into overland flow, two major fluxes are established, the water flow and the associated erosion flow. The process complexity and heterogeneity together with the numerous factor that interact recommend the use of multi-scale approaches for a better understanding. Thus the methodological approach established in this work focus on a hierarchical instrumental implementation at different spatial scales within the same watershed, the Barranc the Carraixet Head Waters, in the vicinity of the city of Valencia, spain. For the instrumentation setting up, four data gathering structures have been designed, from the micro plot scale to the medium size drainage basin of several square kilometres. (Author) 5 refs.

  7. Spectrographic determination of impurities in ammonium bifluoride. III. Study of the processes of vaporization, transport and excitation of the elements Al, B, Cu and Cr; Determinacion espectrografico de impurezas en bifluoruro amonico. III. Estudio de los procesos de volatilizacion, transporte y excitacion de los elementos Al, B, Cu, Cr

    Energy Technology Data Exchange (ETDEWEB)

    Alduan, F A; Roca, M; Capdevila, C

    1979-07-01

    The influences of the processes of vaporization, transport and excitation on the shape of the volatilization-excitation curves and on the values of the spectral-line intensities have been investigated in a method for the spectrographic determination of Al, B, Cu and Cr In ammonium bifluoride samples by direct current are excitation in Scribner type electrodes, with addition of different matrices (graphite, 63203, GeO{sub 2}, MgO and Zn0). The reaction products in the electrode cavity have been identified by X-ray powder diffraction analysis and the percentages of vaporized and diffused element evaluated through analysis by total-burning spectrographic methods. In addition, the values of both the number of particles entering the discharge column and the transport efficiencies have been calculated. Thus, the origin of most observed differences has been explained. (Author) 11 refs.

  8. Aplicación de la resistencia de ruido al estudio de pinturas ricas en zinc

    Directory of Open Access Journals (Sweden)

    Espada Recarey, Luis

    2001-02-01

    Full Text Available Electrochemical Noise has been one of the more useful analysis methods to the study the corrosion processes due to the fact that it is simple and cheap. The objective of this work is to check the efficiency of this technique when it is applied to the study of Rich Zinc Paints and specifically the Noise Resistance parameter which is very efficiency to study metal-electrolyte systems. Then this technique was applied to Rich Zinc Paints systems of which we have already information by means of electrochemical impedance spectroscopy. The outcomes of this paper show the efficiency of applying Noise Resistance technique to explain the behavior of this kind of systems. REVISION 34 Obtención de la tenacidad de fractura dinámica de iniciación de materiales elásticos y lineales Determination of dynamic fracture initiation toughness of linear elastic materials

    La medida e interpretación del ruido electroquímico, REQ se está consolidando como uno de los métodos de análisis más utilizados para la interpretación del fenómeno de la corrosión, por su sencillez y bajo coste frente a otros tipos de metodologías del estudio. El objeto de este trabajo es comprobar la eficacia de la técnica de ruido electroquímico aplicada al estudio de las pinturas ricas en zinc, concretamente, en lo relativo al parámetro "resistencia de ruido" que tan eficaz se ha mostrado en el estudio de sistemas metal desnudoelectrolito. Para ello, se aplicó esta técnica de análisis a muestras de pinturas ricas en zinc, de las que ya se poseía información sobre su comportamiento, a través de la aplicación a las mismas muestras de la impedancia electroquímica, EIE. Los resultados de este articulo muestran la eficacia de la aplicación de la resistencia de ruido, a la hora de explicar el comportamiento de este tipo de sistemas.

  9. Plagas, enfermedades y saberes agrícolas en el Caribe, un estudio de caso

    Directory of Open Access Journals (Sweden)

    Fernández Prieto, Leida

    2011-08-01

    Full Text Available From the new approaches in social studies of science and technology concerned with the analysis of sites where science is developed and how it circulates, I am interested to observe the Caribbean as an island-center in the tropical agronomic knowledge development. For this, I study how the scientific steps were formulated to eradicate the plagues that currently affect more intensely coconut plantations in the Caribbean and Latin America, called Lethal Yellowing Disease of Coconut, which has destroyed the industry crops in many areas. The epidemic first appeared in the nineteenth century in the Caribbean Antilles, which forced local scientists to develop a database of global knowledge. In particular, I observe the process in Cuba from scientific discussions generated at the Royal Academy of Medical Sciences, Physical and Natural Sciences of Havana, as well as the entry of U.S. scientists in the new imperial context of early twentieth century, who expanded their field of study throughout the English and Spanish Caribbean through the exchange of ideas and experiences.

    A partir de los nuevos enfoques en los estudios sociales de la ciencia y la tecnología interesados en el análisis de los sitios donde se produce la ciencia y cómo circula, me interesa observar el Caribe como isla-centro en la construcción del saber agronómico tropical. Para ello estudio cómo se fueron formulando científicamente los pasos a seguir para erradicar una de las plagas que actualmente afectan con más intensidad las plantaciones de cocos del Caribe y América Latina, llamada Amarillamiento Letal del Cocotero (ALC, la cual ha destruido la industria de la copra en muchas zonas productoras. La epidemia apareció por vez primera en el siglo XIX en el Caribe antillano, donde obligó a los científicos locales a elaborar un sistema de conocimiento global. En particular, observo ese proceso en Cuba a partir de los debates científicos generados en la Real

  10. Estudio de Recursos Humanos del Sector Salud en Colombia.

    Directory of Open Access Journals (Sweden)

    Gustavo Malagón Londoño

    1997-12-01

    Full Text Available

    Antecedentes


    Siendo el recurso humano el en cargado de movilizar los demás recursos e impulsarlos de tal manera que conlleve a una dinámica de producción masiva de bienes o servicios, su estudio para llegar a una identificación real, categorización, clasificación; es necesario en cualquier área del conocimiento.

    Frente al nuevo esquema de prestación de servicios propuestos, los profesionales de la salud han perdido aquella posición digna, de respeto y liderazgo dentro del sistema y dentro de su entorno social, lo cual ha llevado a muchos de ellos a desertar de sus oficios originales, con lo cual se llega a la falla en la instauración del Sistema.

    Así mismo la Ley 100 de 1993 que debió ser vista como una nueva oportunidad de negocios dentro de las diferentes profesiones de la salud, se transformó en una seria amenaza para el ejercicio independiente de cada profesión.

    No es apropiado ni satisfactorio dejar un proceso decisorio de tal magnitud en manos de otros sectores que únicamente verán y obtendrán estadísticas del acceso de la población colombiana a estudios superiores y los renglones en los cuales quieren desempeñarse y se desempeñarán en un futuro. El sector de la salud debe tomar un claro papel protagónico frente a la promulgación de políticas de formación y utilización de su recurso humano.

    La Academia Nacional de Medicina consciente del vacío existente en el sector con respecto al tema, ha querido desde su posición estratégica con respecto a las profesiones del área y las universidades, liderar un proceso que conlleve a la organización de un Estudio del recurso humano del Sector Salud en Colombia, tomando como punto de partida el censo de los trabajadores de la salud en todas las áreas, de tal manera que se identifique su disponibilidad, su capacidad, la ubicación, las facilidades laborales en zonas aisladas y su grado de satisfacción, entre otras

  11. O Ambiente Público-Privado no Processo de Tomada de Decisão quanto à Operação Portuária no Brasil: um estudo de casoPublic-Private Environment in the Port Operation Decision-Making Process in Brazil: a case studyLos Sectores Público y Privado en el Proceso de la Toma de Decisiones con respecto a la Operación Portuaria Brasil: un estudio de caso

    Directory of Open Access Journals (Sweden)

    SERRA, Laura Thomaz e

    2009-06-01

    study seeks to evaluate the public-private relationship in the process of decision making in companies, with the objective of analyzing the export process under the perspective of the decisions oriented to the transaction costs management in the company. In short, the research points out that the public-private relationship shows as a result inappropriate environment, tightening the competition on the global scenario. In the case studied the basic parameters around which the attributes are formed, cost and level of service are adversely affected by this relationship. In turn, the institutional environment in Brazil, with regard to port operations, imposes severe transaction costs.RESUMENEn el ámbito de las decisiones de naturaleza microeconómica y de gestión de empresas se observa un creciente reconocimiento de la importancia de las decisiones referentes al transporte, para el desempeño de actividad logística; esto se debe a que ellas asumen trade-offs relevantes con otras funciones y procesos logísticos, como por ejemplo, existencias, almacenamiento y compras, y afectan directamente los resultados de los servicios y costos logísticos. Esto requiere considerar los transportes no como “demanda derivada”, según la tradición neoclásica, sino como variables explicativas de decisiones empresariales. De forma que en el presente artículo se buscó evaluar la relación público privada en la toma de decisiones empresariales, por medio de un estudio de caso, que tuvo por finalidad analizar el proceso de exportación desde la perspectiva de las decisiones empresariales relacionadas con la gestión de los costos de transacción. En resumen, la investigación demuestra que esta relación público privada proyecta un ambiente poco adecuado para la incuestionable exacerbación de la competición en el ámbito global. Es evidente en el caso investigado, que los parámetros básicos alrededor de los cuales se forman los atributos, costos y nivel de servicio, resultan

  12. Dissolution studies of natural analogues spent fuel and U(VI)-Silicon phases of and oxidative alteration process; Estudios de disolucion de analogos naturales de combustible nuclear irradiado y de fases de U(VI)-Silicio representativas de un proceso de alteracion oxidativa

    Energy Technology Data Exchange (ETDEWEB)

    Perez Morales, I

    2000-07-01

    In order to understand the long-term behavior of the nuclear spent fuel in geological repository conditions, we have performed dissolution studies with natural analogues to UO{sub 2} as well as with solid phases representatives of the oxidative alteration pathway of uranium dioxide, as observed in both natural environment and laboratory studies. In all cases, we have studied the influence of the bicarbonate concentration in the dissolution process, as a first approximation to the groundwater composition of a granitic environment, where carbonate is one of the most important complexing agents. As a natural analogue to the nuclear spent fuel some uraninite samples from the Oklo are deposit in Gabon, where chain fission reactions took place 2000 millions years ago, as well as a pitchblende sample from the mine Fe ore deposit, in Salamanca (spain) have been studied. The studies have been performed at 25 and 60 degree centigree and 60 degree centigree, and they have focussed on the determination of both the thermodynamic and the kinetic properties of the different samples studied, using batch and continuous experimental methodologies, respectively. (Author)

  13. Comparative study on systems of residual water treatment in the process industry by evaporation, using fossils fuels or solar energy; Estudio comparativo sobre sistemas de tratamiento de aguas residuales de la industria de procesamiento por evaporacion, utilizando combustibles fosiles o energia solar

    Energy Technology Data Exchange (ETDEWEB)

    Landgrave Romero, Julio; Canseco Contreras, Jose [Facultad de Quimica, UNAM (Mexico)

    1996-07-01

    The residual water treatment of the process industry, nowadays is an imminent necessity in our country. In the present study two different forms are considered to concentrate residual waters: multiple effect evaporation and solar evaporation. The use of solar evaporation lagoons is a good possibility to conserving energy by means of the diminution of fossil fuel consumption. The design basis of the evaporation systems via multiple effect, as well as solar evaporation, the results of the respective sizing and the estimation of the corresponding costs are presented. A practical case is described on the cooking of cotton linters (flock) [Spanish] El tratamiento de aguas residuales de la industria de proceso, hoy en dia es una necesidad inminente en nuestro pais. En el presente trabajo se consideran dos formas distintas para concentrar las aguas residuales: evaporacion de multiple efecto y evaporacion solar. El empleo de lagunas de evaporacion solar es una buena posibilidad para conseguir el ahorro de energia mediante disminucion del consumo de combustibles fosiles. Se presentan las bases de diseno de los sistemas de evaporacion via multiple efecto, asi como solar, los resultados del dimensionamiento respectivo y la estimacion de los costos correspondientes. Se describe un caso practico sobre el cocido de linters de algodon (borra)

  14. Antes de los Estudios Culturales. Robert Warshow y la experiencia

    Directory of Open Access Journals (Sweden)

    Antonio Lastra

    2008-01-01

    Full Text Available Este artículo estudia la influencia del concepto de experiencia que desarrolla Robert Warshow. Warshow ha supuesto el contrapunto americano a las teorías desarrolladas por Walter Benjamien en Europa. Si bien ambos insistieron en la pobreza de la experiencia, como resultado de la tecnificación de la sociedad, Warshow supo introducir matizaciones que influyeron en todo el círculo de intelectuales concentrados en Nueva York durante la etapa de consolidación de los Estudios Culturales. This paper studies the influence of the experience concept by Robert Warshow. Warshow is the American counterpoint to theories developed by Walter Benjamin in Europe. Although both insisted in the poverty of experience, as a result of technique, Warshow knew how to introduce nuances that influenced intellectuals in New York as Cultural Studies emerged.

  15. Tiempo de Respuestas y Experiencia de Usuario Estudio Experimental

    Directory of Open Access Journals (Sweden)

    Delvis Echeverria

    2016-12-01

    Full Text Available La investigación está fundamentada por un estudio experimental, enfocado en la evaluación del comportamiento de los usuarios finales interactuando con tiempos de respuestas en aplicaciones reales. Se tomará como base el aporte de los autores Doherty y Thadani, investigadores de IBM relacionado con los tiempos de respuestas, y las investigaciones de Jacob Nielsen. Se llevarán a cabo un test con usuarios finales, y se expondrán optimizaciones a través de del uso de herramientas que contribuirán a mejorar los tiempos de respuestas de las aplicaciones web. Todo esto engloba una primera instancia de un proyecto que se está llevando a cabo en la empresa GeneXus Consulting, pretendiéndose lograr un punto de encuentro entre el rendimiento de las aplicaciones y la experiencia de usuario.

  16. Primeros resultados sobre el estudio de oscilaciones no radiales

    Science.gov (United States)

    Córsico, A.; Benvenuto, O. G.

    En el Observatorio de La Plata se ha comenzado a elaborar un código de pulsaciones el cual resuelve el problema de las oscilaciones no radiales en el caso adiabático. Dicho código está basado en la técnica de diferencias finitas ampliamente usado en cálculos de estructura y evolución estelar. En este trabajo se presentan los primeros resultados encontrados aplicando el código mencionado al caso de una polítropa de índice n=3. Se presentan los valores de las autofrecuencias y las autofunciones para diferentes modos de pulsación de dicha configuración politrópica. En un futuro próximo, se aplicará este programa al estudio de las pulsaciones no radiales de estrellas enanas blancas.

  17. Estudios inmunologicos en hamsters (Cricetus auratus infectados con Schistosoma mansoni

    Directory of Open Access Journals (Sweden)

    Eduardo Monge

    1986-08-01

    Full Text Available Los resultados de este trabajo muestran que el hamster (Cricetus auratus puede ser utilizado como un modelo experimental para estudios inmunológicos en la infección por Schistosoma mansoni. Los datos obtenidos, relativos a inmunidad concomitante, producción de anticuerpo letal e inmunosupresión se asemejan a los conseguidos en otros modelos experimentales ya establecidos. Estas observaciones indican que el hámster, además de ser un hospedero satisfactorio para el mantenimiento del parásito en el laboratorio, puede ser considerado como un modelo experimental alterno cuyo crecimiento y mantenimiento son relativamente simples y además es un animal de fácil manejo.

  18. Gordailu, Centro de Patrimonio Cultural Mueble de Gipuzkoa. Estudios previos

    Directory of Open Access Journals (Sweden)

    Maite Barrio Olano

    2009-08-01

    Full Text Available La Diputación Foral de Gipuzkoa ha impulsado la creación de una nueva infraestructura cultural: el Centro de Patrimonio Cultural Mueble de Gipuzkoa, denominado Gordailu (palabra en euskera cuyo significado es “depósito”. Este Centro tendrá como misión la gestión integral del patrimonio cultural mueble del territorio, partiendo de un núcleo central, el depósito in situ de colecciones de distinta titularidad. Dentro de la variedad de temas de estudio y análisis que implica un proyecto de esta envergadura, en el presente artículo se relatan los relativos al plan de necesidades, evaluación de colecciones y definición de condiciones medioambientales.

  19. Estudio multifrecuencia del medio interestelar cercano a HD 192281

    Science.gov (United States)

    Arnal, E. M.; Cappa, C.; Cichowolski, S.; Pineault, S.; St-Louis, N.

    Una de las causas que modifica la estructura y dinámica del medio interestelar es la acción que los vientos de las estrellas de gran masa ejercen sobre el mismo. En este trabajo, mediante el uso de datos interferométricos obtenidos en la banda de radio en la transición de 21-cm del Hidrógeno neutro y de imágenes de la emisión de continuo en las bandas de 408 y 1420 MHz, de imágenes HIRES del satélite IRAS en 60 y 100 micrones, y de observaciones de continuo obtenidas con radiotelescopios de disco simple en 2695, 4850 y 8350 MHz se ha realizado un estudio multifrecuencia de los efectos que los vientos estelares de HD 192281, una estrella de tipo espectral O5 Vn((f))p, han tenido sobre el medio interestelar que rodea a la misma.

  20. El abandono en las etapas iniciales de los estudios superiores

    Directory of Open Access Journals (Sweden)

    Norma Zandomeni

    2016-01-01

    Full Text Available En este artículo se presenta una investigación sobre abandono de los estudios superiores realizada en una universidad argentina. Con un abordaje metodológico cuanti-cualitativo, se analizan datos secundarios y se recupera la perspectiva de distintos actores mediante talleres, encuestas y entrevistas. Se aborda el abandono que se produce en el proceso del ingreso y el generado en los dos primeros años, tanto en su cuantificación como en los factores que operan en el mismo. En el primer caso el abandono se asocia principalmente a factores exógenos a la institución mientras que en el segundo toman fuerza factores internos a la universidad.

  1. El experimento formativo en el estudio del lenguaje egocentrico

    OpenAIRE

    Rosalía Montealegre

    1998-01-01

    Este escrito analiza dos métodos experimentales: el genético-modelador y el estadístico (método de evaluación cuantitativa) en el estudio de lenguaje egocéntrico como regulador y planificador de la acción en el niño. Se presentan unas retlexiones teóricas sobre los métodos y su empleo en la investigación realizada por la autora en la Universidad Estatal de Moscú sobre «El papel del lenguaje en la solución de tareas espaciales por niños de edad preescolar». A diferencia de la...

  2. Estudio comparativo del desarrollo de las inteligencias múltiples en alumnos que cursan o no estudios de danza en un conservatorio

    OpenAIRE

    Athanassopoulos-Zamorano, Néstor

    2015-01-01

    El objetivo de este trabajo es analizar si al comparar dos grupos de estudiantes, formados por alumnos que cursan estudios de danza en el conservatorio y alumnos que no los cursan, existen diferencias estadísticamente significativas en las Inteligencias Múltiples. La muestra total del estudio está compuesta por 175 personas, conformando dos grandes grupos, alumnos que estudian danza de manera oficial y alumnos que no estudian danza de manera oficial. Para conocer el nivel de las Inteligenc...

  3. Estudio de preformulación del citrato de dietilcarbamazina

    Directory of Open Access Journals (Sweden)

    Rafael León Rodríguez

    1999-08-01

    Full Text Available Se realizó el estudio de preformulación del citrato de dietilcarbamazina con el objetivo de conocer las características físico-químicas y tecnológicas de este fármaco para el posterior desarrollo de una tableta de 50 mg de dosis, el cual incluyó la determinación del tamaño de partícula micronizada y sin micronizar según el método de microscopia óptica, de manera que quedó demostrado la influencia que presenta éste en la disolución del fármaco in vitro. Se efectuó el estudio de incompatibilidad principio activo-excipientes mediante el método de calorimetría diferencial de barrido; no se encontró ninguna interacción entre éstos, en las condiciones de trabajo experimentales. Se estudiaron otras propiedades físico-químicas y tecnológicas del fármaco.The study of diethycarbamazine citrate preformulation with a view to finding uot the physico - chemical and tachnological characteristics of this pharmaceutical for further production of a 50 mg tablet was performed. This study included the determination of the size of micronized and non-micronized particles by optical microscopy in which the influence of size on the pharmaceutical dissolving in vitro was shown. Drug - excipient incompatibility was analysed using differential scannig calorimetry and no interaction was found under experimental working conditions. Other physico-chemical & technological properties of this pharmaceutical were examined.

  4. Un estudio exploratorio de capacidades emocionales en adultos mayores

    Directory of Open Access Journals (Sweden)

    Zoe S. Bello Dávila

    2012-01-01

    Full Text Available Abstract The study of the emotional world of the elderly is a fundamental requisite for guaranteeing the well-being and health of this group. The objective of this research paper was to characterize a group of elderly people according to their motional capabilities. The group consisted of 122 elderly persons: 107 female, 15 male. All attendants participated in a “Círculo de Abuelos” (Circle of Grandparents. The study was descriptive, it sought to gather information about the responses of older adults in situations of emotional content by completing a questionnaire. The results provided a profile of capacities where areas of strength and weakness are clearly distinguished. Selfconfidence and the possibility of fulfilling projects are among the strengths while the weaknesses are found in interpersonal or intrapersonal managing of emotions. These results have led to a better understanding of the emotional world of the elderly and guide future educational activities. Resumen El estudio del mundo emocional de los adultos mayores es un requisito fundamental para garantizar el estado de bienestar y salud de los mismos. El presente artículo de investigación se fijó como objetivo caracterizar a un grupo de ancianos, en función de sus capacidades emocionales. Los participantes fueron 122 ancianos, en concreto 107 mujeres y 15 hombres, que asisten a un “Círculo de Abuelos”. El estudio fue de tipo descriptivo, pues pretendió recoger información acerca de las respuestas de los adultos mayores ante situaciones de contenido emocional que se exploran por medio de un cuestionario elaborado al efecto. Los resultados ofrecen un perfil de capacidades en que la autoconfianza y la posibilidad de elaborar proyectos se erigen como fortalezas, mientras que las debilidades se expresan en las dificultades para el manejo emocional tanto a nivel intra como interpersonal. Estos resultados han permitido lograr una mejor comprensión del mundo emocional del

  5. Estudio epidemiológico de la dermatitis atópica desde la farmacia comunitaria: Estudio DAFAC

    Directory of Open Access Journals (Sweden)

    Moreno P.

    2014-03-01

    Full Text Available Objetivos: Diseñar y evaluar la utilidad de un protocolo de actuación del farmacéutico comunitario ante las consultas realizadas sobre dermatitis atópica en la farmacia comunitaria, acordando con dermatólogos los criterios de derivación. Conocer los motivos de consulta más habituales y determinar en qué casos constituyen motivo de derivación al médico. Métodos: Estudio epidemiológico realizado en farmacias comunitarias españolas entre febrero del 2011 y enero del 2012. Se incluyeron en el estudio todos los usuarios de las farmacias que consultan un problema de salud dermatológico. Los farmacéuticos participantes, tras recibir formación específica online, aplicaron el protocolo de actuación consensuado. Se registraron los motivos de consulta, los casos de derivación al médico, la existencia de sobreinfecciones, el uso de productos adecuados para el cuidado de la piel. Resultados/Discusión: Participaron 259 farmacéuticos de 15 comunidades autónomas que registraron 688 intervenciones válidas. La distribución por sexo de los pacientes corresponde a 354 (51,5 % de sexo masculino y 334 (48,5 % del femenino, con mayor incidencia de la patología en menores de 14 años. En el 70% de los casos no se requirió la derivación al dermatólogo. Motivos de consulta: prurito, irritación, dificultad para dormir, ansiedad y estrés, y sobreinfección (35%, principal causa de derivación. La elaboración y difusión del protocolo y criterios de derivación, así como los resultados del estudio a los socios de SEFAC (Sociedad Española de Farmacia Comunitaria y al resto de farmacéuticos comunitarios permitirán la resolución de muchos de los casos de dermatitis atópica, contribuyendo así a la mejora de la calidad de vida del paciente.

  6. Modelo de estudio de dos informativas familias colombianas con Síndrome de Usher

    Directory of Open Access Journals (Sweden)

    C. González

    2001-07-01

    Full Text Available Establecer y evaluar un modelo de abordaje para el estudio del Síndrome de Usher, que abarca el diagnóstico clínico de los pacientes, establecimiento y confirmación del subtipo mediante estudios moleculares y posterior correlación genotipo-fenotipo.

  7. Modelo de estudio de dos informativas familias colombianas con Síndrome de Usher

    OpenAIRE

    Tamayo ML.; González C.; Gelvez N.

    2001-01-01

    Establecer y evaluar un modelo de abordaje para el estudio del Síndrome de Usher, que abarca el diagnóstico clínico de los pacientes, establecimiento y confirmación del subtipo mediante estudios moleculares y posterior correlación genotipo-fenotipo.

  8. Los estudios longitudinales en la prevención de las enfermedades cardiovasculares

    Directory of Open Access Journals (Sweden)

    Ignacio Balaguer Vintró

    2004-01-01

    Full Text Available Los estudios longitudinales de cohortes bien definidas han contribuido a la identificación de los factores de riesgo de la cardiopatía coronaria y otras complicaciones clínicas de la aterosclerosis. Después de comentar las conclusiones de los estudios de la aterosclerosis experimental y los factores de riesgo sugeridos por el estudio de una serie de infartos de miocardio en adultos jóvenes en comparación con controles apareados, se expone la metodología, el desarrollo y los resultados de los estudios longitudinales realizados en Estados Unidos desde 1949: Twin Cities, Framingham, Pooling Project, Western Collaborative, Puerto Rico, Evans County, NI-HONSAN, San Francisco, Harvard, Bogalusa y CARDIA. Se presta especial atención a las hipótesis propuestas al inicio del estudio de Framingham y a los obstáculos y cambios para continuar el proyecto después de los primeros veinticuatro años. A continuación se expone el Seven Countries Study, ideado y dirigido por Ancel Keys y primer estudio realizado con metodología centralizada en varios países, y los estudios longitudinales realizados en diversos países de Europa: Whitehall, Manresa, París, British Regional, Northwick Park, Caerphilly, Speedwell, PROCAM. Se analiza el papel de los estudios longitudinales en la metodología de los estudios posteriores: hijos e hijas de los participantes en Framingham, estudios longitudinales basados en cuestionarios, estudios de otros posibles factores de riesgo, prevalencia de factores de riesgo en estudios retrospectivos, ensayos de intervención primaria (MRFT, WHO European Collaborative Trial y el de Goteburgo y la participación de los equipos entrenados en el Proyecto MONICA. Se señalan los temas todavía en debate en relación con la metodología y los resultados de los estudios longitudinales: exámenes periódicos de los participantes en las cohortes de los estudios epidemiológicos, cambios en la definición de nuevos casos de accidentes

  9. Procedimiento de análisis para el estudio del proceso constructivo documentado del Colegio de Corpus Christi de Valencia

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    Lerma, C.

    2014-03-01

    Full Text Available The analysis procedure of the historic building construction sequence must establish the relationship between the documentation study that is conserved and its current state, noting the inconsistencies that occur. This paper focuses on the Seminary-School of Corpus Christi of Valencia, which is 400 years old and retains plenty of original documents relating to its construction. All aspects of the architecture and the construction of the building are analyzed in detail. A graphic study of the costs and three-dimensional hypothesis of the building construction sequence clarify how occurred its construction process. The proposed methodology can be used to study other construction processes in historical buildings.El procedimiento de análisis de la secuencia constructiva de un edificio histórico debe establecer la relación entre el estudio de la documentación que se conserva y el estado actual del mismo, advirtiendo las incongruencias que se produzcan. Este trabajo se centra en el Colegio-Seminario de Corpus Christi de Valencia, que cuenta con una antigüedad de 400 años y conserva abundante documentación original relativa a su construcción. Se analizan con detalle todos los aspectos relacionados con la arquitectura y la construcción del edificio, aclarando cómo se produjo el proceso de construcción, facilitando un estudio gráfico de los gastos y una hipótesis tridimensional de la secuencia constructiva. La metodología que se propone puede servir para el estudio de procesos constructivos en otros edificios históricos.

  10. Centro de Estudios para el Desarrollo Rural Sustentable y la Soberanía Alimentaria (CEDRSSA. Estudios e investigaciones: nueva ruralidad; enfoques y propuestas para América Latina

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    Rosa Inés Babilonia Ballesteros

    2014-01-01

    Full Text Available Centro de Estudios para el Desarrollo Rural Sustentable y la Soberanía Alimentaria (CEDRSSA. Estudios e investigaciones:nueva ruralidad; enfoques y propuestas para América Latina.

  11. The use of radioactive inserts in the study of metal deformation during tube-making processes; Emploi de fils metalliques radioactifs pour l'etude des deformations des metaux pendant la fabrication de tubes; Ispol'zovanie radioaktivnykh vtulok dlya izucheniya deformatsii metallov pri protsesse izgotovleniya trub; Empleo de insertos radiactivos en el estudio de la deformacion de los metales durante la fabricacion de tubos

    Energy Technology Data Exchange (ETDEWEB)

    Davison, W H.T. [Tube Investments Research Laboratories, Cambridge (United Kingdom)

    1962-01-15

    This paper reports on the use of radioactive inserts in studying the deformation of steel in hot rotary piercing for tube making. Iron or steel wires are activated in the pile, inserted in tubes drilled in iron or steel billets, which are then processed under normal production conditions. Careful monitoring shows that the radiation exposures and contamination of the plant are quite negligible. On cooling, the billets are scanned in the laboratory, using a collimated scintillationcounter mounted normal to the surface of the pierced billet, and isodose curves show the shape of the deformed insert normal to the tube surface. A similar scan of a section normal to the axis of the tube gives a second plot; from a combination of the two plots, a three-dimensional picture is obtained. Some results are quoted, but the main emphasis of the paper is on the experimental technique, which has quite general applicability to metal deformation studies. The insert must be located within a millimeter or thereabouts, for which a higher resolution is required than is normal in medical scanning techniques. The problems of achieving this are discussed in terms of {gamma}-energy, collimator design and energy discrimination. (author) [French] Le present memoire traite de l'emploi de fils metalliques radioactifs pour l'etude de la deformation de l'acier durant le forage rotatif a chaud en vue de la fabrication des tubes. Des fils de fer ou d'acier sont actives dans un reacteur et places dans des cavites tabulaires menagees a l'interieur de billettes de fer ou d'acier que l'on transforme ensuite dans les conditions de fabrication normales. Un controle radiologique minutieux montre que les dangers d'irradiation et de contamination dans l'usine sont pratiquement negligeables. Apres refroidissement, les billettes sont examinees en laboratoire au moyen d'un compteur a scintillations avec collimateur de faible ouverture, monte de facon que l'axe soit perpendiculaire a la surface de la billette

  12. Experimental Study of the Process of Neutron Thermalization in Time in Aqueous Moderators; Etude Experimentale du Processus de Thermalisation des Neutrons en Fonction du Temps dans les Ralentisseurs Contenant de l'Eau; Ehksperimental'noe izuchenie protsessa termalizatsii nejtronov vo vremeni v vodorodosoderzhashchikh zamedlitelyakh; Estudio Experimental del Proceso de Termalizacion de Neutrones en Funcion del Tiempo en Moderadores Acuosos

    Energy Technology Data Exchange (ETDEWEB)

    Ishmaev, S. N.; Mostovoj, V. I.; Sadikov, I. P.; Chernyshov, A. A. [Gosudarstvennyj Komitet po Ispol' zovaniju Atomnoj Ehnergii SSSR, Institut Atomnoj Ehnergii Im. I.V.Kurchatova, Moskva, SSSR (Russian Federation)

    1965-08-15

    Z gN bol'shogo razmera (V{sup 2} = 3,8 * 10{sup -2} sm{sup 2}), otnosjatsja k oblasti vremen zamedlenija (t > 30 mksek), gde srednjaja energija nejtronov nizhe 0,13 jev i obmen energiej mezhdu nejtronnym gazom i sredoj proishodit, v osnovnom, za schet vozbuzhdenija akusticheskih kolebanij reshetki. V jetoj oblasti process termalizacii idet sravnitel'no medlenno i vremja, neobhodimoe dlja ustanovlenija ravnovesnogo spektra nejtronov, okazyvaetsja porjadka 400 mksek. V sborke iz ZrH malogo razmera (V{sup 2} Asymptotically-Equal-To 20 * 10{sup -2} sm{sup 2}) projavljaetsja sil'nyj jeffekt diffuzionnogo ohlazhdenija. Spektry, izmerennye pri bol'shih vremenah zamedlenija, sil'no otlichajutsja ot maksvellovskogo raspredelenija s temperaturoj sredy. Vremja, neobhodimoe dlja ustanovlenija ravnovesnogo energeticheskogo raspredelenija, vozrastaet s umen'sheniem razmerov sistemy. energeticheskie spektry nejtronov v vode (V{sup 2} = 2,56 * 10{sup -2} sm{sup -2}) predstavleny dlja vremen zamedlenija bol'shih 13 mksek, gde iskazhenija za schet razreshenija neznachitel'ny. Forma spektra polnost'ju sovpadaet s raspredeleniem Maksvella pri komnatnoj temperature lish' spustja Tilde-Operator 100 mksek posle vspyshki bystryh nejtronov. Nabljudaemoe v jeksperimente vremja ustanovlenija ravnovesnogo spektra nejtronov v 3 - 4 raza bol'she, chem dajut raschety, vypolnennye razlichnymi avtorami. Obsuzhdajutsja vozmozhnye prichiny rashozhdenij. (author)

  13. STRATEGY FORMATION AS A COLLECTIVE PROCESS AND MULTIPLE CULTURAL PERSPECTIVES: A QUALITATIVE STUDY IN AN EXCLUSIVE FURNITURE STORE FORMACIÓN DE ESTRATEGIA COMO UN PROCESO COLECTIVO Y MÚLTIPLES PERSPECTIVAS CULTURALES: UN ESTUDIO CUALITATIVO EN UNA TIENDA DE MUEBLES EXCLUSIVA FORMAÇÃO DE ESTRATÉGIA COMO UM PROCESSO COLETIVO E MÚLTIPLAS PERSPECTIVAS CULTURAIS: UM ESTUDO QUALITATIVO EM UMA LOJA EXCLUSIVA DE MÓVEIS

    Directory of Open Access Journals (Sweden)

    Fabiano Larentis

    2013-01-01

    Full Text Available Understanding an organization and its strategy formation processes implies to go beyond its tangible elements, eg, physical structure and product offerings. The organizational complexity demands a deeper understanding to explain some intangibles such as organizational culture. Organizational culture as a system of symbols and meanings (ALVESSONl, 2003, defines how strongly a company conducts its business. Moreover, according to Mintzberg, Ahlstrand and Lampel (2000, the formation of strategy can be seen as a process of social interaction based on common understandings to members of an organization, presenting cultural characteristics. Moreover, considering the studies of Martin and Frost (2001, organizational culture has three different perspectives: the perspectives of integration, differentiation and fragmentation. Through a qualitative research in an exclusive planned furniture store, this study identifies and evaluates the cultural perspectives offered by Martin and Frost, considering the formation of strategy as a collective (cultural process. The results demonstrate, although the predominance of differentiation perspective (subcultures, its relation to different situations of integration (consensus, and fragmentation (a mixture of consensus and conflict produces complementary outputs, interfering in various ways to realize the strategies: plan, pattern, position, perspective and trick. From this analysis, this paper provides a better understanding of some cultural elements that underlie the formation of an organization's strategies, especially when cultural perspectives are considered.Comprender una organización y sus procesos de formación de estrategia implica ir más allá de sus elementos tangibles, como estructura física y productos ofrecidos. La complejidad organizacional exige una comprensión más profunda de sus características, que implica necesariamente el entendimiento de algunos elementos intangibles, como

  14. Estudio de las cualidades inmunoestimulantes de nuevas bacterias probióticas asociadas al cultivo de lv

    OpenAIRE

    Gullian, Mariel; Rodríguez, Jenny

    2002-01-01

    Estudio de las cualidades inmunoestimulantes de nuevas bacterias probióticas asociadas al cultivo de LV Estudio de las cualidades inmunoestimulantes de nuevas bacterias probióticas asociadas al cultivo de LV

  15. Estudio de impacto ambiental de un aeródromo

    Directory of Open Access Journals (Sweden)

    Gómez Orea, Domingo

    1996-04-01

    actividades, estimulan las iniciativas locales y revalorizan los territorios contiguos. Se expone en este artículo una síntesis del Estudio de Impacto Ambiental de un futuro aeródromo, actualmente en fase de proyecto, cuya vinculación al procedimiento administrativo de EIA, viene determinada por la legislación específica vigente en la actualidad: R.D. 1302/86. El documento técnico sobre el que se hace el estudio corresponde al plan especial del aeródromo. Un criterio básico en la concepción de aeropuertos y aeródromos privados es la compatibilidad con la habitabilidad del entorno y con las condiciones ecológicas y paisajísticas, criterio que debe intervenir en la orientación de las pistas, trayectorias en la maniobras de despegue y aterrizaje... incluso en la localización y diseño de estacionamientos, hangares y demás instalaciones. Esta idea aconseja concebir el proyecto con sensibilidad ambiental, desde las primeras fases, evitando repercutir la responsabilidad en este tema al estudio de impacto ambiental. Se adopta un estilo propio de la divulgación, en la idea de que más que los datos técnicos, que sólo interesan a la tramitación del proyecto, el lector encontrará mayor utilidad en los aspectos metodológicos y en aquéllos que han permitido al equipo redactor formar criterio sobre los costes y beneficios ambientales del proyecto y sobre la aceptabilidad de éste, en suma. Se ha utilizado una metodología clásica, de acuerdo con los requerimientos del reglamento de EIA.

  16. Etica, la academia, y las organizaciones sociales: estudio de caso

    Directory of Open Access Journals (Sweden)

    Sans, Mónica

    2005-01-01

    Full Text Available La Constitución de la República Oriental del Uruguay señala, en su Artículo 70: "El Estado propenderá al desarrollo de la investigación científica...". Sin embargo, la repatriación, a instancias de una organización de descendientes de indígenas autotitulados charrúas (ADENCH y con apoyo del Parlamento, de los restos del cacique charrúa Vaimaca Perú, muerto en cautividad en Francia en 1833, motivó controversias acerca de si los restos del mismo podían ser estudiados. Esta discusión no fue académica, si bien hubo derivaciones que influyeron sobre otras investigaciones, sino que fue suscitada por dicha organización la cual se opuso al estudio de los restos llegando a interponer varios recursos legales; uno de éstos, que condujo a un juicio, motivó un fallo histórico de un Tribunal de Apelaciones a favor del "interés general" por conocer "sin ataduras, sin secretos y sin misterios, la identidad de nuestro pueblo...". Sin embargo, y sin que se hiciera mención de dicha sentencia, el Parlamento aprobó posteriormente la Ley 17.767 que prohíbe expresamente la realización de "experimentos y estudios científicos" en los restos de Vaimaca Perú, violando según algunos autores, la propia Constitución y planteando, además de la discordancia entre dos Poderes del Estado, la oposición entre diversos intereses. A partir de estos hechos se propone analizar los valores éticos que subyacen a la investigación, si deben plantearse limitaciones a ésta y de ser así, cuáles, e incluso, cómo se debe normar sobre las mismas.

  17. Estudio de caso y aprendizaje cooperativo en la universidad

    Directory of Open Access Journals (Sweden)

    Pedro Aramendi Jauregui

    2014-01-01

    Full Text Available Diseñar los planes de estudio en función del desarrollo de competencias demanda cambios metodológicos importantes. El método de caso es una de las estrategias de enseñanza y aprendizaje más adecuadas para desarrollar las competencias orientadas al trabajo en equipo y al fomento de la colaboración. El artículo que se presenta es un resumen del proyecto de innovación “El estudio de caso y el aprendizaje cooperativo en la universidad” (PIE-2010-2012 financiado por la Universidad del País Vasco. El objetivo general del proyecto es implementar el método de caso en las asignaturas de Organización de Centros (SocioEducativos (titulaciones de Pedagogía y Educación Social. MÉTODO: Se han seleccionado dos grupos de segundo curso de la titulación de Educación Social (uno en euskera y otro en castellano y otros dos del mismo curso de Pedagogía en ambos idiomas. En uno de los grupos de cada titulación se trabajaron técnicas de dinámica de grupo y en otro se desarrolló la estrategia del método de caso sin la ayuda de ellas. El alumnado evaluó la experiencia mediante un diario de grupo. RESULTADOS: Los datos indican que el alumnado está satisfecho con las actividades de aprendizaje realizadas en clase, las competencias de la titulación se desarrollan de manera satisfactoria y el funcionamiento de los grupos experimentales, en general, es mejor que el de los grupos control. La eficacia del trabajo grupal está estrechamente vinculada con variables de tipo relacional. DISCUSIÓN: La cooperación, la ayuda y la supervisión de tareas entre los miembros del grupo fomentan la interacción y el sentimiento de seguridad entre los estudiantes que tienen dificultades para lograr los objetivos de aprendizaje. Un alumno o alumna que se siente respaldado por su grupo a nivel académico, afectivo y relacional probablemente va a percibir un contexto de trabajo más seguro, inclusivo y acogedor.

  18. Obesidad infantil: un nuevo enfoque para su estudio

    Directory of Open Access Journals (Sweden)

    Fabian Leonardo Muñoz Muñoz

    2017-01-01

    Full Text Available La Organización Mundial de la Salud ( OMS ha catalogado la obesidad infantil como la mayor crisis de la salud pública en el mundo. En su informe de 2014 indica que se registraron cuarenta y dos millones de niños menores de 5 años con sobrepeso; de ellos, treinta y cinco millones viven en países en desarrollo. La obesidad infantil es una enfermedad que tiene como origen una cadena causal compleja, de etiología multifactorial, en la que interactúan factores individuales y contextuales; en ellos el niño se encuentra inmerso, y ejercen influencia sobre su comportamiento, en diversos entornos y en diferentes niveles de jerarquía. Esta revisión presenta una descripción de algunos estudios y esfuerzos realizados para carac - terizar, prevenir y controlar la epidemia de obesidad en esta etapa del ciclo vital. Se propone, así mismo, un abordaje para el estudio de la obesidad, denominado ‘Enfoque de sistemas complejos’, el cual permite analizar cómo los factores individuales y contextuales se relacionan, y cómo esa interacción genera nuevas propiedades que no pueden explicarse a partir de los procedimientos habituales de análisis epidemiológico; para ello será necesario el trabajo en equipo, con enfoque multidisciplinario, que incluya médicos, pediatras, nutricionistas, epidemiólogos, salubristas, estadísticos, ingenieros, entre otros profesionales, que aporten sus conocimientos y habilidades, que permitan entender la obesidad como un fenómeno dinámi - co que requiere ser abordado con un enfoque integral e integrador, que trascienda la mirada reduccionista de los factores de riesgo del proceso salud – enfermedad. Solo entonces se podrá mejorar la capacidad para comprender la salud desde una perspectiva clásica hasta un sistema verdaderamente dinámico.

  19. LOS GENES BRCA1 y BRCA2. ESTUDIO MOLECULAR

    Directory of Open Access Journals (Sweden)

    N. Alonso

    2006-11-01

    Full Text Available RESUMENEn los últimos años, se realizaron numerosos estudios para establecer la predisposición hereditaria al cáncer y las alteraciones mutacionales a nivel de genes susceptibles de originar cáncer de mama y ovario. En 1994 se identificaron los genes BRCA1 (Breast Cancer Gene 1 y BRCA2 (Breast Cancer Gene 2 como susceptibles de cáncer de mama y ovario. En la actualidad se sabe que las mutaciones en BRCA1 y BRCA2 están lejos de explicar la totalidad de los casos de cáncer de mama y/o ovario, y a pesar de que se postulan alteraciones mutacionales en otros genes como CHEK2, TP53 y PTEN, el BRCA1 y BRCA2, siguen teniendo su importancia y utilidad en la valoración del riesgo de predisposición hereditaria. Aunque las cifras son variables según los distintos estudios y autores, se trata en cualquier caso de porcentajes importantes. Entre el 15 y el 85% de las mujeres portadoras de mutación BRCA 1 o BRCA 2 tienen riesgo de desarrollar un cáncer de mama y entre un 10 y 60% de desarrollar un cáncer de ovario. ABSTRACT:In the last years, numerous studies were made to establish the hereditary predisposition to the cancer and the mutationals alterations at level of genes susceptible to originate breast and ovarian cancers. In 1994 genes BRCA1 (Breast Cancer Gene 1 and BRCA2 were identified (Breast Cancer Gene 2 as susceptible of both of breast and ovarian cancers. At the present time, it is knows that the mutations in BRCA 1 and BRCA 2 are far from explaining the totality of the cases of breast cancer and/or ovary, and although mutationals alterations in other genes like CHEK2, TP53 and PTEN, the BRCA1 and BRCA2 are postulated, they continue having his importance and utility in the valuation of the risk of hereditary predisposition. Correlations between both BRCA1 and BRCA2 levels with tumour grade metastasis and prognostic accuracy. Between 15 and 85% of the carrying women of mutation BRCA 1 or BRCA 2 have risk of developing a cancer of breast

  20. Razonamiento covariacional en el estudio de funciones cuadráticas

    Directory of Open Access Journals (Sweden)

    Jhony Alexander Villa-Ochoa

    2012-10-01

    Full Text Available En este artículo se usa el marco conceptual de Carlson et al. (2003 para discutir los resultados de un estudio de caso, el cual describe la forma como un estudiante razona covariacionalmente al enfrentarse a situaciones de variación asociadas a funciones cuadráticas. El estudio se ideó para desarrollar una línea convergente de indagación (Yin, 2009, la cual se centró en las descripciones que el estudiante realizaba a medida que abordaba las situaciones diseñadas para el estudio; dichas descripciones fueron trianguladas con las producciones escritas y los elementos teóricos. Desde las acciones que el estudiante evidenció, se pudo observar que el proceso de razonamiento covariacional no es un proceso lineal pero sí recursivo. Así mismo, este estudio de caso pone en evidencia el hecho de que existen estudiantes que pueden aproximarse a una interpretación variacional de las concavidades de una gráfica, sin que ello exija un estudio previo del cálculo diferencial. Del estudio se desprenden algunas implicaciones tanto para el marco conceptual abordado en este estudio como para el diseño de situaciones orientadas al aula de clase.

  1. Familias reconstituidas: Un estudio sobre las nuevas estructuras familiares

    Directory of Open Access Journals (Sweden)

    ISABEL ESPINAR FELLMANN

    2003-01-01

    Full Text Available En la mayoría de las investigaciones sobre la familia, los estudios se han centrado en las familias intactas o primeras familias. Sin embargo, es cada vez mayor el número de familias cuya configuración incluye un segundo matrimonio e hijos de una unión anterior (i.e. familias reconstituidas. En la consolidación del complejo proceso de ajuste de estas familias se han identificado unas fuentes comunes de estrés relacionadas principalmente con la formación de un nuevo sistema familiar. El objetivo de este artículo es realizar una revisión de las variables que inciden en el bienestar y/o malestar psicológico sobre una muestra de padrastros y madrastras españoles en el seno de estas nuevas configuraciones familiares. En una primera fase se realizaron, desde un diseño cualitativo, 20 entrevistas en profundidad a una muestra de padrastros y madrastras. En una segunda fase, se diseñó un cuestionario que fue respondido por 77 sujetos con la finalidad de analizar la posible relación entre alguna de las variables estudiadas.

  2. Actividad metacognitiva en estudiantes universitarios: un estudio preliminar

    Directory of Open Access Journals (Sweden)

    Kelly Cantillo Anaya

    2014-01-01

    Full Text Available Esta investigación tuvo como objetivo dar cuenta de niveles de actividad metacognitiva en estudiantes universitarios, estableciendo diferencias en función de variables como la edad y semestre acadé - mico de los participantes, para lo cual se utilizó el Cuestionario de Autorreporte de Actividad Metacognitiva. En el estudio participa - ron 95 estudiantes de ambos sexos, que cursaban primero, quinto y décimo semestre en un programa de psicología de una universidad pública. Los datos arrojados permiten establecer que la actividad metacognitiva, en relación tanto con el semestre como con la edad en la muestra estudiada, se ubica en un nivel medio bajo. En ninguno de los semestres, ni en los rangos de edad se registran niveles altos en los componentes de Conciencia, Control y Autopoiesis. No se encontró correlación significativa entre actividad metacognitiva y semestre aca - démico cursado, como tampoco con la edad.

  3. Breve estudio comparativo de los modernos medios de transporte terrestre

    Directory of Open Access Journals (Sweden)

    de Andrés Conde, M. Carmen

    1974-04-01

    Full Text Available Interurban Planning Section General Division of Highways and Subsidiary Roads In this study a series of data regarding the modern means of transport, available at present, are being compiled and compared in order to facilitate the choice of the most suitable one for each case. In order to understand better the various using conditions we have listed the most prominent known characteristics of each of them in alphabetical order in the definitions. With some of these characteristics as a point of departure, such as speed and capacity we have obtained criteria as to the application field of every means of transport.En este estudio se recopilan y comparan una serie de datos actualmente disponibles sobre modernos medios de transporte terrestre, con objeto de facilitar la elección del más adecuado en cada caso. Para ayudar a la comprensión de sus condiciones de explotación, se han resumido en las definiciones las características conocidas más destacadas de cada uno de ellos, ordenándolos por orden alfabético. A partir de algunas de estas características de explotación, como velocidad y capacidad, se han obtenido criterios sobre el campo de aplicación de cada medio de transporte.

  4. INTELIGENCIA Y RENDIMIENTO DEPORTIVO: UN ESTUDIO SOBRE LA INTELIGENCIA EMOCIONAL

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    Gerardo Araya Vargas

    2001-06-01

    Full Text Available Este estudio tuvo el propósito de determinar la correlación entre los índices de inteligencia tradicional (razonamiento analógico, C. I. e inteligencia emocional (C.E. con el rendimiento deportivo. Participaron 10 basketbolistas, entre los 17 y 25 años (media = 20.1 años, todas mujeres. Se les aplicó pruebas de C. I. (Raven y de C. E. (Goleman y luego se sometió a los sujetos a dos pruebas de precisión (tiro libre y tiro de tres puntos y posteriormente el entrenador evaluó mediante la Escala de Rendimiento Emocional Percibido por el Técnico, la capacidad de manejo emocional de las basketbolistas en situación de juego. El puntaje de C. E. se correlacionó significativamente (r=0.71; p < 0.05 con la precisión en el tiro de tres puntos. En los otros casos las correlaciones fueron bajas y no significativas. La evaluación del entrenador mostró (rbis = -0.87; p < 0.05 correlación significativa y negativa entre el índice C. E. y la percepción del efecto negativo de las emociones sobre el rendimiento, por tanto existe evidencia preliminar de que este índice podría ser valioso como predictor del rendimiento en circunstancias de alta exigencia.

  5. Cuidadores de Cartagena y su contexto de cuidado: estudio comparativo

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    Amparo Montalvo-Prieto

    2009-01-01

    Full Text Available Objetivo: comparar las características sociodemográficas y de relación de cuidado de cuidadores de niños y adultos con enfermedades crónicas discapacitantes de Cartagena, Colombia. Método: estudio descriptivo, cuantitativo, de corte transversal. Se realizó un muestreo no probabilístico, por conveniencia, entre el 2006 y el 2007. Participaron 300 cuidadores: 98 de niños y 202 de adultos con enfermedades crónicas. Se utilizó la encuesta de caracterización de los cuidadores. Para describir las asociaciones entre las variables seleccionadas se empleó el análisis factorial de correspondencias múltiples. Resultados: ser cuidador de niños se asoció con ser padre o madre, ejercer el rol durante 37 meses o más, dedicarse a actividades del hogar y estar en unión libre. Ser cuidador de adultos se asoció con pacientes mayores, llevar 36 meses o menos como cuidador, ser hijo del paciente y estar casado. Conclusiones: las variables que mejor diferenciaron los dos grupos de cuidadores fueron nivel socioeconómico, estado civil y tiempo de dedicación al cuidado. El grupo de cuidadores de adultos presentó mayor variabilidad en las distintas categorías estudiadas.

  6. humano. Un estudio en 3º de ESO

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    Jesús Ramón Girón Gambero

    2015-01-01

    alimentos. La investigación se ha llevado a cabo con el objetivo de estudiar el desempeño del alumnado en elanálisis de un video publicitario en un contexto real de aula, mediante un estudio de caso en 3º de EducaciónSecundaria Obligatoria. Se utiliza un anuncio televisivo que incluye un modelo para explicar cómo actúa unconocido producto probiótico en el organismo. Para obtener datos y evidencias se han utilizado el diario delprofesor, grabaciones en vídeo de las clases y los cuadernos de trabajo de los estudiantes. En los resultados sedestacan los elementos más fácilmente interpretables y las dificultades del alumnado para comprender el modelodel anuncio. Finalmente, como implicación educativa, se formulan algunas ideas que podrían formar parte de un modelo escolar que explique cómo actúan las defensas en el intestino humano, integrando aquellos aspectos positivos identificados en el anuncio con algunos elementos de los propios modelos de los alumnos y las ideas científicas al respecto.

  7. Estudio sobre las motivaciones para recorrer el Camino de Santiago

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    Antonio Granero Gallegos

    2007-09-01

    Full Text Available Este estudio se ha centrado en conocer las motivaciones del viajero jacobeo actual, mayor de 15 años, que andando o en bicicleta recorre el Camino de Santiago, analizando las relaciones referentes a las necesidades de evasión de la vida cotidiana y la experiencia activa a través de la actividad físico-deportiva. La investigación se ha desarrollado mediante la aplicación de un cuestionario autoadministrado a una muestra de 1.071 sujetos, utilizando el procedimiento de muestreo estratificado polietápico con afijación proporcional, con un margen de error muestral del ± 3 % y un nivel de confianza del 95,5%. Los resultados han puesto de manifiesto la adaptación de esta ruta, tradicionalmente recorrida por motivos religiosos, a la época contemporánea, constituyendo, en la actualidad, un escenario en el que están presentes los distintos elementos del ocio y las actividades físico-deportivo-recreativas en el medio natural, vinculada con el turismo rural, deportivo, de aventura o cultural, y donde el sujeto busca también alejarse del cotidiano estrés urbano.

  8. Proyectos educativos: estudio comparativo entre Venezuela y Colombia

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    Mineira Finol

    2005-09-01

    Full Text Available El Proyecto Educativo Integral Comunitario (PEIC y el Proyecto Educativo Institucional constituyen la categoría de comparación entre Venezuela y Colombia; a tal fin, este estudio de tipo comparativo-documental se centró en determinar congruencias y discrepancias entre PEIC y PEI, utilizando el método comparativo estructurado en cuatro fases: descripción, interpretación, yuxtaposición y comparación, propuesto por Hilker y Bereday (1972 Entre los resultados se tienen: congruencias en cuanto surgen sobre las bases de diagnóstico externos efectuados por organismos internacionales UNESCO, CEPAL ante la inefectividad y baja productividad de ambos países; a nivel interno responden a políticas educativas reformadoras de currículum y leyes,. Discrepancias referidas al tiempo de implementación; en Venezuela a partir de 1996; Colombia en 1992; este último, consolidó las bases para la participación a través del gobierno escolar y de un sistema de evaluación institucional, muy incipiente en Venezuela.

  9. Estudio de los componentes antioxidantes y actividad antioxidante en tomates

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    Luz M. Zapata

    2007-01-01

    Full Text Available El objetivo de este estudio fue investigar la concentración de los componentes antioxidantes presentes en el tomate y la actividad antioxidante total durante su crecimiento, almacenamiento, maduración y comercialización. Los antioxidantes analizados fueron: licopeno, β-caroteno, ácido L-ascórbico y fenoles totales. La actividad antioxidante se midió utilizando los métodos: "Ferric reducing/antioxidant power" (FRAP y decoloración del β-caroteno. Los antioxidantes ácido L-ascórbico, licopeno y β-caroteno fueron 2,6 veces mayor en la comercialización respecto de los valores iniciales, mientras que la actividad antioxidante en la última etapa fue 2,1 y 3,5 veces más alta que en el crecimiento para los métodos FRAP y decoloración de b-caroteno, respectivamente. En consecuencia, el consumidor dispone de un fruto rico en antioxidantes, lo que le atribuye la capacidad de captación de los radicales libres presentes en nuestro cuerpo que podrían disminuir los riesgos que contraer enfermedades crónicas.

  10. Auto concept y rasgos de personalidad: Un estudio correlacional

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    Débora Cecílio Fernandes

    Full Text Available Este estudio ha investigado las relaciones entre los rasgos de personalidad y auto concepto. Fueron aplicadas la Escala de Traços de Personalidade para Crianças y la Escala de Autoconceito Infanto-Juvenil en 389 niños, con edad de 8 a 10 años de escuelas públicas y particulares. Se han hallado diferencias de sexo para casi todas las medidas hechas. Para los varones, ha sido observado correlaciones positivas entre extroversión y auto conceptos escolar y familiar, neuroticismo y psicoticismo con auto conceptos personal y social, y sociabilidad con auto concepto familiar; y correlaciones negativas entre extroversión y auto concepto social, psicoticismo y auto concepto familiar, y sociabilidad y auto concepto personal. Para las niñas, fueron observadas correlaciones positivas entre el auto concepto social y psicoticismo y sociabilidad con el familiar; y correlaciones negativas entre extroversión y el social, psicoticismo y neuroticismo con el familiar. Los análisis de los grupos extremos confirmaron los datos encontrados.

  11. La periferia: voz y sentido en los estudios urbanos

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    Daniel Hiernaux

    2004-01-01

    Full Text Available En este trabajo exploramos la voz ‘periferia’ el contexto latinoamericano: cómo cambia tiempo que el fenómeno denotado se transforma y cómo agrega nuevos componentes en el diálogo entre la palabra y el fenómeno. Por ser una voz reciente, también revisamos otras voces emparentadas (‘arrabal’ y ‘suburbio’, reconstruyendo el uso y sentido cada una de ellas y la sustitución de unas otras hasta que aparece la palabra ‘periferia’. La expansión de las metrópolis y la constitución de amplios espacios periféricos sido de mucho interés en los estudios urbanos. No obstante, hay numerosos aspectos poco analizados. En el primer apartado revisamos voces antecedentes. Luego reconstruimos contexto en el que se comienza a referir territorio de expansión de la ciudad con palabra ‘periferia’, así como sus implicaciones en cuanto al sentido de la voz. En el apartado siguiente analizamos las dimensiones que incorporó la voz ‘periferia’ en relación procesos urbanos; para terminar con los desafíos actuales en torno a ese territorio las formas de nombrarlo

  12. VIOLENCIAS PATRIARCALES EN LOS ENTORNOS VIRTUALES. UN ESTUDIO CON ADOLESCENTES

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    Trinidad Donoso-Vázquez

    2016-05-01

    Full Text Available En este estudio se analizan las violencias patriarcales que tienen lugar en los entornos virtuales. La muestra está formada por 155 adolescentes de primer curso de educación segundaria obligatoria (12 años y cuarto de educación secundaria obligatoria (16 años de la ciudad de Barcelona (España. Un 51,4% eran chicas y un 48,6% chicos. Se aplicó una escala tipo Likert de tres alternativas de respuesta. La escala constaba de 23 ítems que describían agresiones realizadas en los entornos virtuales (nunca, algunas veces, muchas veces. La escala muestra una fiabilidad alta en relación al índice de Alpha de Cronbach, (0,887 lo que indica una adecuada consistencia interna de sus ítems. Los resultados demuestran que internet es un espacio generizado, donde los ataques se realizan por no cumplir los impuestos normativos asignados a los géneros. Es preocupante que en todos los indicadores los chicos puntúen más alto que las chicas menos en los ítems que tienen que ver con el control y posesión de la pareja y en estereotipos sexistas femeninos.

  13. Convivencia escolar. Un estudio sobre buenas prácticas

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    María José Caballero Grande

    2010-06-01

    Full Text Available Este trabajo de investigación se centra en la convivencia escolar y puede enmarcarse en la línea de educación para la paz.La finalidad del mismo ha sido conocer algunas peculiaridades de prácticas educativas  que fomentan la paz y la convivencia en el ámbito escolar. Para esto, se ha recogido información, mediante entrevista estructurada, en diez centros  educativos de la provincia de Granada, acogidos a la Red Andaluza  “Escuela, espacio de paz”, sobre la puesta en marcha, desarrollo y evaluación de actuaciones encaminadas hacia la cohesión del grupo, la gestión democrática de normas, la educación en valores, las habilidades socioemocionales y la regulación pacífica de conflictos.Del análisis de las entrevistas, se desprende que hay una serie de medidas y actuaciones que se llevan a cabo en los centros educativos que favorecen la buena convivencia. La necesidad de cooperación entre todos los actores intervinientes en el proceso educativo o  la importancia de sistematizar e institucionalizar el trabajo que eduque para el fomento de una cultura de paz, conforman entre otras las conclusiones a las que se llega en este estudio.

  14. Estudio de Caso de la Experiencia Subjetiva del Cuerpo

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    Edith Flores Pérez

    2007-01-01

    Full Text Available Estudio de caso que explora la experiencia subjetiva del cuerpo en la trayectoria con el uso de drogas a través del relato de vida. Se utilizó la entrevista abierta bajo los supuestos metodológicos del relato autobiográfico. Se propuso al participante “Hablar de sí mismo y contar su experiencia con el uso de drogas”. La estrategia analítica se diseñó con base en la propuesta de Barthes (1990 para el análisis estructural de los relatos. Los resultados muestran que en el relato emerge una subjetividad que articula modos de haber sido y ser cuerpo como instancias constitutivas de la existencia. Desde el punto de vista de la corporalidad, la trayectoria con el uso de drogas deviene en una relación de saber-poder con el propio cuerpo, donde la experiencia subjetiva se entreteje en una red de controles y normas sociales. El análisis del cuerpo como experiencia enriquece la comprensión de prácticas como el uso de drogas.

  15. Razonamiento covariacional en el estudio de funciones cuadráticas

    OpenAIRE

    Jhony Alexander Villa-Ochoa

    2012-01-01

    En este artículo se usa el marco conceptual de Carlson et al. (2003) para discutir los resultados de un estudio de caso, el cual describe la forma como un estudiante razona covariacionalmente al enfrentarse a situaciones de variación asociadas a funciones cuadráticas. El estudio se ideó para desarrollar una línea convergente de indagación (Yin, 2009), la cual se centró en las descripciones que el estudiante realizaba a medida que abordaba las situaciones diseñadas para el estudio; dichas ...

  16. ESTUDIO SOBRE LA DINÁMICA TEMPORAL DE MATERIAL PARTICULADO PM 10 EMITIDO EN COCHABAMBA, BOLIVIA

    OpenAIRE

    Salini Calderón, Giovanni Angelo; Medina Mitma, Evelin Jhovana

    2017-01-01

    RESUMEN En este documento se presenta un estudio de series temporales de PM10 que muestran la mala calidad del aire en Cochabamba, mediante parámetros estadísticos usados en estudios sobre dinámica no lineal. El promedio diario de PM10 sigue patrones similares al de grandes ciudades que poseen altos índices de contaminación ambiental. Uno de los parámetros resultó del mismo orden y característica que los presentados en trabajos similares sobre el estudio de caoticidad en variables de contamin...

  17. Estudio de la cultura, sus manifestaciones y efectos en una Red Inter-Organizacional

    OpenAIRE

    Guevara Alarcon, Laura Natalie

    2015-01-01

    El presente trabajo tiene como propósito el estudio de la cultura, y el impacto que tiene esta en una red inter-organizacional. Para esto se realizó un estudio documental en el cual se hizo una revisión bibliográfica de los principales conceptos relacionados con la cultura y el enfoque de trabajo en red. Asimismo para dar cumplimiento al objetivo de la investigación, se realizó el análisis de varios estudios empíricos que muestran las relaciones entre cultura y redes y que a su vez re...

  18. Estudio de la variabilidad genética en camélidos bolivianos

    OpenAIRE

    Barreta Pinto, Julia

    2013-01-01

    El estudio de los camélidos sudamericanos es de gran interés en los países andinoscomo Perú, Bolivia, Chile, Argentina, debido a su importante valor económico y suimportancia en el mantenimiento y desarrollo de las poblaciones rurales en dichos países. Dada la falta de estudios genéticos centrados en las poblaciones de camélidos quehabitan en Bolivia, y la necesidad de realizar una valoración de la diversidad genética deestas poblaciones, la presente Tesis doctoral ha abordado el estudio gené...

  19. El estudio de las psicopatologías en Puerto Rico

    OpenAIRE

    Alfonso Martínez Taboas

    1995-01-01

    El estudio de las psicopatologías en Puerto Rico fue un campo poco productivo durante las primeras siete décadas de este siglo. Durante este término de tiempo las publicaciones profesionales fueron exiguas y mayormente anecdóticas. Sin embargo, la década de los años ochenta fue una muy productiva en términos de publicaciones profesionales y estudios de corte empírico. Principalmente se destacan una serie programática de estudios epidemiológicos que abarcan poblaciones de niñ...

  20. Caractereisticas de la dieta de una muestra de adolescentes con sobrepeso y obesidad: estudio EVASYON

    OpenAIRE

    Suárez, N.; Wärnberg, Julia; Zapatera, Belén; Campoy, Cristina; Martí, Amelia; Garragori, J. M.; Marcos, Ascensión

    2014-01-01

    La prevalencia de sobrepeso y obesidad en niños y adolescentes ha aumentado en los últimos años en nuestro país, liderando la prevalencia europea de obesidad infantil. El propósito de este estudio es obtener información sobre la calidad de la dieta de una muestra de adolescentes con sobrepeso y obesidad antes de comenzar un estudio de intervención integral de nutrición, actividad física y psicología EVASYON es un estudio multicéntrico realizado en 5 hospitales españoles (Granada, Madrid, Pamp...

  1. Estudio del estado del arte y perspectivas de los metales críticos

    OpenAIRE

    Lluch Fruns, Nuria

    2015-01-01

    El presente proyecto esta centrado en el estudio del estado del arte y las perspectivas de los metales críticos, seleccionados por su estado geopolítico, riesgo que supone su escasez en el suministro y su correspondiente riesgo medioambiental. Los metales en los que se basa el siguiente estudio aparecen listados a continuación (por orden alfabético): Antimonio, Berilio, Cobalto, Galio, Indio, Metales del Grupo Platino, Niobio, Tantalio y Tungsteno. El estudio consta de una defi...

  2. Estudio por espectroscopia Raman-IR del estado de orden en materia carbonosa

    OpenAIRE

    Carbajo Hijarrubia, Juan

    2016-01-01

    La espectroscopia Raman es una técnica analítica no destructiva y no intrusiva recientemente introducida en el estudio de la paleobiología. En esta línea se pretende emplear está técnica en el estudio de la posible vida en Marte y en el estudio de la vida en la Tierra. El empleo de la espectroscopia micro Raman nos permite analizar en detalle las muestras a la escala típica del grano mineral. La espectroscopia Raman muestra la información vibracional de los grupos moleculares y su ordenamient...

  3. Síndrome de lisis tumoral en un paciente con cáncer de riñón tratado con sunitinib Tumor lysis syndrome in a patient with a renal carcinoma treated with sunitinib

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    Ezequiel Rodríguez-Reimúndes

    2011-04-01

    Full Text Available El síndrome de lisis tumoral (SLT es un trastorno metabólico que ocurre como consecuencia de una destrucción celular masiva. Se caracteriza por la presencia de hiperuricemia, hiperfosfatemia, hipocalcemia e hiperkalemia, y predispone al desarrollo de insuficiencia renal aguda. En la mayoría de los casos el SLT ocurre luego de instaurarse un tratamiento antitumoral y es más frecuente en tumores de alto grado de malignidad y alta sensibilidad a la quimioterapia. Presentamos el caso de un paciente con diagnóstico de cáncer de riñón recidivado que presenta un SLT e insuficiencia renal aguda luego de iniciar tratamiento con sunitinib.The tumor mor lysis syndrome (TLS is a metabolic disorder resulting from a massive tumor breakdown. It is characterized by hyperuricemia, hyperphosphatemia, hypocalcemia and hyperkalemia and predisposes to acute renal failure. TLS usually occurs after the initiation of cytotoxic therapy and is more frequent in the case of neoplasias with a high proliferative rate or that are highly chemo-sensitive. We report the case of a man with a recurrent kidney cancer who presented with a TLS and acute renal failure after initiation of sunitinib.

  4. ESTUDIO CONTRASTIVO DE LA ELIPSIS EN INGLÉS TÉCNICO Y LITERARIO ESTUDIO CONTRASTIVO DE LA ELIPSIS EN INGLÉS TÉCNICO Y LITERARIO

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    Asunción Jaime

    2007-05-01

    Full Text Available Various studies have shown that reading comprehension is one of the most complex communicative skills for Spanish students, who have difficulties in accurately understanding the message conveyed in the written text, in particular in those cases in which the use of cohesion devices based on the principle of "verbatim recoverability" is involved. One, and probably the most complex of such textual resources is ellipsis, as it lacks a distinctive mark in the text indicating the omission of some part of the information. The main objective of the present work is to gain a deeper understanding of the process of textual ellipsis through the comparative analysis of literary vs. technical texts for teaching purposes.Diversos estudios han demostrado que la comprensión lectora es una de las destrezas lingüísticas de mayor complejidad para nuestros estudiantes que tienen problemas a la hora de descifrar los recursos textuales que utiliza la lengua inglesa para transmitir el mensaje, basados en la economía del lenguaje. Parte de ese complejo entramado textual consiste en omitir algunas unidades de información que son recuperables del resto del discurso. Pero, dado que este recurso discursivo de la elisión carece de marca distintiva en el texto, su identificación resulta más complicada que la de otros aspectos retóricos. Por ello hemos querido profundizar en la comprensión del proceso de elisión mediante un análisis comparativo de utilización de este recurso en inglés literario y científico con fines fundamentalmente pedagógicos.

  5. Local lysis with Alteplase for the treatment of acute embolic leg ischemia following the use of the Duett {sup trademark} closure device: preliminary results; Lokale Alteplase-Lyse zur Therapie der akuten embolischen Beinischaemie nach Einsatz des Duett {sup trademark} -Verschluss-Systems: vorlaeufige Ergebnisse

    Energy Technology Data Exchange (ETDEWEB)

    Schuermann, K.; Buecker, A.; Wingen, M.; Tacke, J.; Wein, B.; Guenther, R.W. [Klinik fuer Radiologische Diagnostik des Universitaetsklinikums der RWTH Aachen (Germany); Janssens, U. [Medizinische Klinik I (Kardiologie) des Universitaetsklinikums der RWTH Aachen (Germany)

    2004-04-01

    Purpose: To analyze retrospectively the result of the alteplase lysis therapy of embolic complications following the use of the Duett closure device. Methods and Materials: For 3.5 years, the Duett closure device was used in 1,398 angiographies to close the femoral puncture site. The Duett device consists of a balloon and a liquid procoagulant containing collagen and thrombin, which is injected into the puncture tract under endovascular balloon protection of the arterial puncture site. In 9 patients (0.64%), the procoagulant was incidentally injected into the femoral artery causing acute leg ischemia. Eight patients received local lysis therapy with alteplase via a contralateral femoral access. One patient underwent surgery. On average, 21 mg alteplase (4-35 mg) were administered within 14 h (4-21 h). The course of the lysis was followed angiographyically and clinically. All patients were inteerviewed by telephone 23 months (4-35 months) later.Results: In 3 patients, lysis was complete. In 5 patients, only little thrombotic material remained. In all patients, symptoms of ischemia resolved completely within the first hours after initiation of lysis. In 5 cases, bleeding occurred at the puncture site closed with the Duett device during lysis, including development of a false aneurysm in 2 cases. Complications led to premature termination (n=2) or interruption of the lysis (n=3). All complications were treated conservatively. Clinically, long-term sequelae were paresthesia and hypoesthesia in the lower leg and foot in 2 patients treated with lysis, and in the patient who underwent surgery. (orig.) [German] Ziel: Das Ergebnis der Alteplase-Lysetherapie von Embolien nach Einsatz des Duett-Verschluss-Systems wurde retrospektiv untersucht. Methoden: Innerhalb von 3,5 Jahren wurde nach 1398 Angiographien das Duett-System zum Verschluss der femoralen Punktionsstelle eingesetzt. Das System besteht aus einem Ballon und einem fluessigen Prokoagulans (Kollagen, Thrombin), das

  6. Estudios de economía y cooperación social. Un estudio experimental con estudiantes universitarios en Chile

    Directory of Open Access Journals (Sweden)

    Javier Núñez

    2009-01-01

    Full Text Available Este artículo aporta nuevas pruebas experimentales del efecto de estudiar economía en dos dimensiones de la cooperación social: la confianza y la reciprocidad. Estas dimensiones son aproximadas por medio del conocido juego de confianza. Empleando técnicas de muestreo e igualación (matching, y un reclutamiento de sujetos por invitación selectiva en vez de convocatoria abierta, el trabajo permite separar el efecto asociado al estudio de la economía del efecto asociado al potencial sesgo de selección en las preferencias de quienes escogen estudiar economía. La muestra está compuesta por estudiantes universitarios de economía y de otras carreras con escaso o nulo contenido de economía en Chile. Los resultados muestran que los montos enviados por los estudiantes de economía en la primera etapa del juego de confianza son significativamente menores en comparación con los estudiantes de otras carreras, lo que indica una menor propensión de los primeros a confiar en los demás. A su vez, encontramos una menor inclinación de los economistas a devolver los montos prometidos, lo que muestra una menor tendencia a la reciprocidad de la confianza depositada en ellos en el juego de confianza.

  7. Los estudios de fecundidad en poblaciones contemporáneas como referentes directos para los estudios paleodemográficos

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    Patricia Olga Hernández Espinoza

    2014-01-01

    Full Text Available Las limitantes del trabajo paleodemográfico, como la obtención de datos sobre la fecundidad de las sociedades antiguas, han impulsado el desarrollo de estrategias metodológicas para obtener esta información con algún grado de certeza. En este estudio contrastamos dos tipos de metodologías para obtener datos sobre fecundidad: la paleodemográfica basada en el análisis bioarqueológico de una serie esquelética maya prehispánica, utilizando el modelo de Weiss, y la metodología demográfica moderna aplicada a datos censales de Quintana Roo, con los que se calcularon varios indicadores de fecundidad. Los resultados muestran que el método estadístico que hemos aplicado en nuestras investigaciones permite tener un acercamiento confiable a los niveles de fecundidad y mortalidad que pudieron haber tenido estos grupos en el pasado, pues las estimaciones paleodemográficas presentan valores consistentes con la información obtenida de los censos, con un cálculo de cuatro a seis hijos para el grupo maya prehispánico de Chac Mool.

  8. Estudio neuroepidemiológico nacional (EPINEURO colombiano

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    Gustavo Pradilla A.

    2003-08-01

    Full Text Available OBJETIVO: Determinar la prevalencia de ocho problemas neurológicos -migraña, enfermedad cerebrovascular, enfermedad de Parkinson, neuropatía periférica, trastornos del desarrollo neurológico, epilepsia, demencia y secuelas de traumatismo craneoencefálico- en Colombia como parte del estudio neuroepidemiológico nacional (EPINEURO. MÉTODOS: Se realizó una encuesta transversal en dos fases entre septiembre de 1995 y agosto de 1996. En la primera fase se identificó a las personas que podían estar padeciendo alguna enfermedad neurológica y en la segunda se hizo el diagnóstico específico. Se examinó una muestra de 8 910 personas distribuidas en proporción con la población de cada una de las cinco zonas geográficas de Colombia, que son las regiones central, sudoccidental, noroccidental, oriental y la costa caribe. La evaluación se realizó según una versión modificada del protocolo de neuroepidemiología de la Organización Mundial de la Salud, además de un examen físico simplificado. A los posibles pacientes de demencia identificados se les aplicó un examen neuropsicológico, diseñado para este estudio, con el fin de profundizar en los aspectos cognoscitivos. Se aplicaron las escalas de Hachinski, Yesavage y Blessed y los criterios diagnósticos del NINCDS-ADRDA para la enfermedad de Alzheimer, los del NINDS-AIREN para la demencia de origen vascular y los del DSM-IV para todo tipo de demencia. Se realizó una encuesta para determinar si había secuelas de traumatismo craneoencefálico. Las personas mayores de 12 años fueron evaluadas por neurólogos de adultos y los menores de 12 años por un neuropediatra. Se calcularon las prevalencias de trastornos neurológicos por edad y sexo, tanto por región como para el país en su totalidad, y sus intervalos de confianza de 95%. RESULTADOS: La prevalencia por 1 000 habitantes de los trastornos investigados fueron las siguientes: migraña, 71,2 (intervalo de confianza de 95% [IC95

  9. Estudio comparativo entre bibliotecas universitarias latinoamericanas y estadounidenses

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    Arenas Franco, María Luisa

    2002-06-01

    Full Text Available The paper presents the first results of a comparative study done by the Library System (SIBUC and the Industrial and System Engineering Department of the Pontificia Universidad Católica de Chile. The objective of the study is the development of indicators for University Libraries to compare themselves and to identify their relative position in relation to other peer or leader institutions at the national, regional and international level. With the collaboration of four Chilean and two Latin-American prestigious Universities data weal obtained. In adition, information regarding ten American Universities was obtained from the online version of US News. Nineteen indicators for the following three main areas: budget, relationship between resources/Faculty and Studens, and Efficiency on the use of resources were developed so that the institutions, for bench-marking purpose, are able to evaluate their own strategic position and to improve their weaknesses and maintain their strengths. Finally, benefits that can be obtained from the use of the nineteen indicators are stated for SIBUC, as well as for other Latin-American University Libraries.

    Se presentan los primeros resultrados de un estudio comparativo (benchmarking iniciado por el Sistema de Bibliotecas de la Pontificia Universidad Católica de Chile (SIBUC en conjunto con el Departamento de Ingeniería Industrial y de Sistemas de esta misma universidad. Su objetivo es el establecimiento de índices para bibliotecas universitarias, que les permitan a través del tiempo compararse e identificar su posición relativa respecto a otras instituciones pares o líderes en el ámbito nacional, latinoamericano e internacional. para ello, se contó con la colaboración en Chile de cuatro de sus principales universidades, en Latinoamérica de dos universidades de prestigio, y de estados Unidos se obtuvo a través de internet de diez universidades incluidas en ranking por la US News. El estudio arroj

  10. Estudio del modelo de desgaste propuesto por Archard

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    FABIO ALEXANDER SUÁREZ BUSTAMANTE

    2005-01-01

    Full Text Available El comportamiento de componentes de máquinas y de materiales necesita ser optimizado para reducir los costos de operación en las plantas industriales. Este hecho ha impulsado la Tribología, un área relacionada con el estudio de la fricción, la lubricación y el desgaste. Uno de los principales objetivos de esta área del conocimiento es elaborar modelos matemáticos para expresar la variación del desgaste durante el contacto de superficies en movimiento, con el fin de predecir la vida útil de los elementos de máquinas. El modelo de Archard para desgaste por deslizamiento en seco, formulado en 1953, es un modelo clásico que plantea una variación del desgaste inversamente proporcional a la dureza del material y directamente proporcional a la carga aplicada. En el presente trabajo, se ha utilizado el método de modelamiento de problemas físicos, propuesto por Asbhy, para analizar los modelos propuestos por Archard (Modelo de contactos múltiples en superficies rugosas y modelo de desgaste mecánico. Con base en él se ha podido realizar un análisis profundo a dichos modelos, mostrando un panorama más amplio del modelo de Archard; se discuten aspectos no mostrados en los textos y artículos de tribología, donde el tratamiento dado a este modelo resulta ser superficial, desconociendo las limitaciones y la perspectiva que tuvo Archard sobre los aspectos que deberían considerarse en un modelo de desgaste.

  11. Estudio taxonómico del genero Byttneria Loefling (Sterculiaceae

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    Carmen Lelia Cristóbal

    1976-01-01

    Full Text Available Byttneria es el único género de la tríbu Byttnerieae representado en América' cuyo estudio taxonómico no había sido encarado en su totalidad. Las contribuciones más importantes son las que' se hicieron en floras, como la de K. Schumann (1886, para Brasil b la de Arenes (1959, para Madagascar. Aunque se trata de importantes trabajos, la limitación de los mismos, impidió valorizar en su medida los cáracteres morfológicos que definen las secciones de este género. Es así COmo ahora'al comparar morfólógicamente las 131 especies que lo componen, se propone la división del género en 6 secciones, de las cuales 4 s?n descriptas por pnmera vez.En este trabajo el tratamiento a nivel de especie no es uniforme. He revisado críticamente, en primer término, las 79 especies americanas, de las cuales 27 resultaron nuevas para la ciencia. Con respecto a las 52 restantes que son extraamericanas estudié la mayor parte de los tipos y procedí de la siguiente manera. Acepto las 27 especies delimitadas por Arenes endémicas de Mada:gascar, e incluyo una lista de las mismas. Enumero las 6 especies de Africa continental, de las cuales una vive también en América. Analizo y redescribó B. herbacea, endemismo de la India, que constituye por sí sola la sección Gerontogaea. Finalmente, enumero bajo la sección Vahihara las 19 especies asiáticas restantes, propias de Indochina e Indonesia, con algunas notas críticas.

  12. Estudio Preliminar del Género Hevea en Colombia

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    Evans Schultes Richard

    1946-04-01

    Full Text Available Aunque en el territorio colombiano se han venido explotando los árboles caucheros del género Hevea durante casi medio siglo, solo ha sido en los últimos años cuando los botánicos han podido estudiar científicamente las especies en este país. En el presente artículo me limito a exponer de manera suscinta la identificación específica y la distribución geográfica del género Hevea en Colombia, valiéndome de las identificaciones botánicas preliminares que se han hecho hasta ahora, así como de los datos que he recogido personalmente y las informaciones que me han sido suministradas en fuentes fidedignas. Es de lamentarse la falta de exploración botánica en la hoya del río Inírida y sus afluentes que por ser, junto con el Guaviare, la región situada más al noreste de la zona de extensión del género Hevea, deja una laguna en los conocimientos que actualmente poseemos sobre la distribución geográfica de este género botánico. Igualmente carecemos de datos botánicos respecto de los ríos de menor importancia tales como el Yarí, el Cahuinarí y el Cotuhé, aunque sí los tenemos de las regiones mencionadas, lo mismo de otras vastas extensiones de la Amazonía Colombiana, podremos completar a satisfacción el estudio cuyo esbozo provisional presento en este trabajo.

  13. Estudio de la anurofauna del municipio de Ibagué

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    M. H. Bernal

    2000-07-01

    Full Text Available Colombia es considerada, en el mundo, el país más rico en especies de anfibios (Lynch, 1998. De las 4.300 especies registradas en el planeta, Colombia presenta 583 (Ruiz et al., 1996. Esto refleja la gran riqueza que el país tiene respecto a la diversidad  no sólo de anfibios, sino también de otros grupos de animales y plantas. Entre los anfibios el mayor número de especies se encuentra en el orden Anura, los comúnmente conocidos como ranas y sapos, le sigue el orden Urodela (salamandras y por ultimo esta el Apoda (cecilias. En Colombia se han reportado 540 especies de anuros, pertenecientes a 56 géneros y 9 familias (Ruiz et 01., 1996. Por otra parte, entre las regiones de Colombia con mayor aporte a la diversidad de anuros esta la Cordillera Central. Estimulados por esta riqueza de anuros y dada las amplias zonas del país aún desconocidas a nivel de inventarios, entre ellas el municipio de Ibagué, el cual por su posición geográfica en la Cordillera Central y por su variedad de niveles altitudinales, ecosistemas y el hábitat para el albergue de los anuros, se llevó a cabo el presente estudio, con el propósito de reconocer el aporte de este municipio a la diversidad descrita con respecto a la Cordillera Central ya Colombia.

  14. Estudio descriptivo de algunas variables emocionales en las personas mayores

    Directory of Open Access Journals (Sweden)

    Igone Etxeberria

    2016-08-01

    Full Text Available Se analizó el funcionamiento emocional (afecto positivo y negativo, satisfacción con la vida, soledad y estrategias de regulación de las personas centenarias en comparación con el de personas de menor edad (65-74, 75-84 y 85-94 años. Participaron en el estudio 257 personas mayores sin deterioro cognitivo y sin dependencia para las Actividades de la Vida Diaria (AVD. Los resultados revelaron que, en las personas centenarias, se producía una disminución de la afectividad positiva en comparación con los grupos de 65 a 74 años y de 75 a 84 años, pero no así con el de 85 a 94 años. Además, presentaban menor afecto negativo y más satisfacción con la vida (en comparación con el grupo de 65 a 74 años, al tiempo que manifestaban sentirse más solas (en comparación con los grupos de 65 a 74 años y de 75 a 84 años. Con respecto a las estrategias de regulación, en general, las personas centenarias utilizan en menor medida las estrategias proactivas y la solución de problemas y en mayor medida las pasivas, al menos en la regulación de la tristeza. En conclusión, a pesar de experimentar menos afecto positivo y más soledad se produce cierta disminución en el afecto negativo, e incluso cierto aumento de la satisfacción con la vida. En lo que respecta a la regulación emocional, las personas centenarias utilizan menos las estrategias proactivas y más las pasivas (tristeza.

  15. El cuerpo, en el campo de estudio de la fisioterapia

    Directory of Open Access Journals (Sweden)

    Adriana Prieto Rodríguez

    2005-04-01

    Full Text Available Objetivo: describir el concepto de cuerpo como parte de la investigación sobre la categoría disponibilidad corporal y su explicación para la fisioterapia. Material y métodos: investigación teórica, en la cual se aplicó el método fenomenológico para la definición de las categorías emergentes, se desarrollaron las fases de documentación, procesamiento analítico de los documentos e interpretación y construcción de subcategorías. Se empleó la técnica de análisis documental a una muestra de 59 documentos de 82 reunidos. Como criterio de validez interna para el proceso de interpretación se efectuó lectura de los documentos por parte de cada una de las investigadoras y posterior contrastación. Resultados: se identificaron dos enfoques interpretativos, el monismo y el dualismo, que generan relaciones centradas en la condición material o espiritual del cuerpo. Desde el dualismo, el estudio del cuerpo en la profesión se dirige a su capacidad funcional y adaptación orgánica; desde el monismo, la fisioterapia reconoce al cuerpo con respecto a su condición de lenguaje, de interacción social y de conciencia. Conclusiones: se reconoce el cuerpo como un lenguaje humano con implicaciones teóricas y metodológicas para la profesión. Ello exige integrar los aspectos orgánicos, simbólicos y psíquicos del cuerpo, en los procesos de formación de pregrado y avanzada.

  16. ANÁLISIS DEL CONTRATO DE LECTURA DE DOS PUBLICACIONES PERIÓDICAS DE CIENCIAS SOCIALES DE ARGENTINA: ESTUDIOS. REVISTA DEL CENTRO DE ESTUDIOS AVANZADOS (UNC Y ESTUDIOS SOCIALES (UNL

    Directory of Open Access Journals (Sweden)

    Florencia María Páez

    2012-01-01

    Full Text Available Pretendemos con este trabajo efectuar un análisis del contrato de lectura de dos publicaciones periódicas de Argentina que se inscriben en el campo de estudios de las ciencias sociales. Partimos de las ideas de Eliseo Verón, esbozadas en “Cuando leer es hacer: la enunciación en el discurso de la prensa gráfica” (1984, y realizamos una apropiación de las categorías del autor teniendo en cuenta las particularidades propias de las revistas científicas y el modo de funcionamiento del subcampo de publicaciones de ciencias sociales del país, en íntima relación con los capitales en juego en el campo científico en general. Hemos escogido uno de los últimos números de cada una de las revistas Estudios del Centro de Estudios Avanzados de la UNC, y Estudios Sociales, de la Universidad Nacional del Litoral. Los criterios que fueron atendidos al momento de la elección de estas revistas apuntaron a la factibilidad de efectuar una comparación entre los contratos de lectura de ambas, según la propuesta de Verón en torno a este tipo de análisis. Este estudio nos permite profundizar nuestra comprensión del funcionamiento de la comunicación científica gráfica, puntualmente posibilita conocer las estrategias que las publicaciones despliegan para captar a los académicos (potenciales lectores y autores en las revistas y para convencer de la calidad científica de su trabajo editorial a los encargados de elaborar índices y catálogos de publicación.

  17. ESTUDIO DEL EFECTO DE LA CRANEOACUPUNTURA EN PACIENTES CON ENFERMEDAD VASCULAR CEREBRAL. (EVC)

    OpenAIRE

    PEREZ BRAVO, MIGUEL

    2009-01-01

    LA ENFERMEDAD VASCULAR CEREBRAL (EVC) EN MEXICO ES UN IMPORTANTE PROBLEMA DE SALUD PUBLICA, ES LA TERCERA CAUSA DE MUERTE Y LA SEGUNDA EN PRODUCIR INCAPACIDAD NEUROLOGICA. EL OBJETIVO DE ESTE ESTUDIO FUE EVALUAR EL EFECTO DE LA CRANEOACUPUNTURA EN PACIENTES CON ENFERMEDAD VASCULAR CEREBRAL (ZHONG FENG). EL PRESENTE ES UN ESTUDIO EXPERIMENTAL, PROSPECTIVO Y LONGITUDINAL, REALIZADO EN EL HOSPITAL DEL ISSSTE DE LA CIUDAD DE PUEBLA, A UN GRUPO DE 11 PACIENTES DE AMBOS SEXOS, CON...

  18. Situación de los Estudios Clínicos en Colombia

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    Alexander Carreño Dueñas

    2013-06-01

    Full Text Available Antecedentes: Los estudios clínicos son un tipo de investigación que ayudan a la ciencia médica a encontrar nuevas formas de prevenir, detectar, diagnosticar o tratar enfermedades y hoy en día son los estudios que aportan la mayor evidencia científica frente al uso de nuevas terapias farmacológicas. Objetivo: Describir la situación actual de los estudios clínicos en Colombia. Métodos: Se consultaron las bases de datos de registro de estudios clínicos, se realizó una revisión de los aspectos regulatorios y la normatividad relacionada. Resultados: no es posible determinar la cantidad exacta de estudios clínicos registrados en Colombia debido a que no se cuenta con un sistema de registro. Los estudios clínicos registrados difieren en las bases de datos consultadas. Desde 1995 a 2013 se identificaron 738 ensayos únicos, el 90% de ellos fueron patrocinados por industrias farmacéuticas, 62% fueron fase III y se han desarrollado principalmente en enfermedades crónicas como el cáncer, las enfermedades cardiovasculares y las degenerativas. Conclusiones: Colombia carece de un sistema de registro de estudios clínicos que permita realizar un seguimiento adecuado por parte de la comunidad y los profesionales de la salud. Debido a que el mayor porcentaje de estos estudios han sido patrocinados por la Industria Farmacéutica, se hace necesario fomentar y desarrollar estrategias que permitan el desarrollo de investigación propia

  19. Estudio de las propiedades mecánicas del sistema óseo

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    Alvaro Mendoza G.

    1991-03-01

    Full Text Available Los estudios adelantados fueron realizados para el área de Biomecánica, tratando de que su desarrollo fuera lo más científico posible; y aplicado al estudio del sistema óseo del hombre, ya que él posee un material que tiene un comportamiento que hace posible las aplicaciones de conceptos mecánicos y físicos de la Ingenlerla para su análisis.

  20. Estudio de las propiedades mecánicas del sistema óseo

    OpenAIRE

    Alvaro Mendoza G.

    2011-01-01

    Los estudios adelantados fueron realizados para el área de Biomecánica, tratando de que su desarrollo fuera lo más científico posible; y aplicado al estudio del sistema óseo del hombre, ya que él posee un material que tiene un comportamiento que hace posible las aplicaciones de conceptos mecánicos y físicos de la Ingenlerla para su análisis.

  1. TENDENCIAS TRANSDISCIPLINARIAS EN LOS ESTUDIOS MÉTRICOS DE LA INFORMACIÓN Y SU RELACIÓN CON LA GESTIÓN DE LA INFORMACIÓN Y DEL CONOCIMIENTO

    Directory of Open Access Journals (Sweden)

    Salvador Gorbea-Portal

    2013-06-01

    Full Text Available Se perfila una nueva tendencia en los Estudios Métricos de la Información a partir del enfoque transdisciplinario propio de la naturaleza de este tipo de estudio, a través del cual se identifican y definen un conjunto de procesos y metodologías convergentes con este tipo de estudio, conocidas como: KDD (Knowledge Discovery Database – Descubrimiento de Conocimiento en Bases de Datos, la Minería de Datos, de Texto y de la WWW, la Bibliomining (Bibliominería y el OLAP (Online Analytical Processing (Procesamiento Analítico en Línea, utilizadas para el descubrimiento de conocimiento en los repositorios de datos (Data Warehouse, así como la vinculación de esta convergencia con los Sistemas de Gestión de Información y de Conocimiento, finalmente se propone un modelo en el que se muestran estas relaciones.

  2. Aportaciones de la paleohistología humana al estudio de biomateriales

    Directory of Open Access Journals (Sweden)

    Nacarino Meneses, C.

    2012-12-01

    Full Text Available The deep study of archaeological human bone could provide relevant information to biomaterials science, as it could tell how the implant process of bioglasses and bioresorbable ceramics is. In this paper, we propose to study, by means of different microscopic, spectroscopic, and X-ray diffraction techniques, the histological and mineral bone variability throughout ontogeny. Extrapolating this data, we could have a better knowledge of biodegradable materials implant. In different ages, we could notice different tissues in cortical bone: fibrolamellar bone is characteristic of early stages of life while secondary or harvesian bone is in adult individuals. Raman and infrared spectroscopy suggest an increase of critallinity in the inorganic matrix during live. Finally, the X-ray diffraction study of bone tissue shows β-calcium phosphate and hydroxyapatite as the main mineral bone components.

    El estudio en profundidad del hueso humano arqueológico puede aportar información relevante a la ciencia de los biomateriales, al informar sobre la evolución del proceso de implante de biovidrios o biocerámicas reabsorbibles. En este trabajo, se propone una aproximación, mediante diferentes técnicas de microscopía, espectroscopía y difracción de rayos X, a la variabilidad histológica y mineral del tejido óseo en el transcurso de la ontogenia y así, poder realizar una extrapolación que permita comprender mejor el implante de materiales biodegradables. Respecto a la variabilidad histológica, se han observado diferentes tipos de tejidos en la cortical del hueso en las distintas edades, siendo el hueso fibrolaminar principal en etapas tempranas de la vida, y el hueso secundario o haversiano en etapas adultas. Los análisis de espectroscopía Raman e infrarroja indican un aumento en la cristalinidad de la parte inorgánica del hueso a lo largo del ciclo vital. Por último, el estudio del tejido óseo mediante difracción de rayos X muestra la

  3. REDUCTION OF EXCESS SLUDGE PRODUCTION IN AN ACTIVATED SLUDGE SYSTEM BASED ON LYSIS-CRYPTIC GROWTH, UNCOUPLING METABOLISM AND FOLIC ACID ADDITION

    Directory of Open Access Journals (Sweden)

    V. F. Velho

    Full Text Available Abstract The following sludge reduction alternatives were tested in wastewater biological reactors: oxic-settling-anaerobic (OSA-process; ultrasonic disintegration (UD; chlorination (CH; 3,3',4',5-tetrachlorosalicylanilide (TCS; and folic acid (FA. Compared to the control system, UD reduced 55% of the sludge production, and greater substrate and nutrient removal efficiency was achieved. CH worsened the sludge settleability and increased the SVI values; the system achieved 25% of sludge reduction. OSA showed 50% and 60% of sludge reduction after 16 and 10 hours under anaerobic conditions, respectively. The observed sludge yield during TCS addition was decreased by 40%, and the sludge settleability worsened. FA presented the highest sludge reduction (75%, and the system improved the nutrient removal efficiency by 30% compared to the control system and maintained the sludge properties. Acute toxicity conducted with Daphnia magna classified the effluent from the sludge reduction systems as non-toxic for discharge into water sources.

  4. Corte de placas solares con láser. Estudio de modificaciones microestructurales

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    Ramírez Del Valle, A. J.

    2003-06-01

    Full Text Available In photovoltaic solar cells manufacturing process, system breaks are produced. Recycling improves the material profitability. In this sense, manufacturing of components with non-straight shapes is not feasible using traditional methods. The processing of these materials through Nd-YAG laser, allows manufacturing with any geometric shape. This work shows a study of solar cells cutting process with laser. First of all, it is necessary to carry out a parameter optimization in order to make an accurate material cutting. The microstructure of the cutting area and possible microcompositional changes were studied by scanning electronic miscroscopy (SEM and X-ray analysis (EDX.

    En el proceso de fabricación de placas fotovoltaicas se producen roturas de las células solares, por tanto, el reciclaje es necesario para mejorar la rentabilidad de este proceso, además, la fabricación de piezas de formas curvas no es factible por los métodos tradicionales. El procesado de estos materiales mediante un láser de Nd-YAG, permitirá la fabricación en cualquier forma geométrica además del mejor aprovechamiento de las placas desechadas por estar rotas. El presente trabajo muestra un estudio del proceso de corte de placas solares con láser. En primer lugar se procede a la optimización de los parámetros necesarios para realizar el corte de este material. La microestructura de la zona de corte y los posibles cambios microcomposicionales que se hayan podido producir, se estudian mediante microscopía electrónica de barrido, con microanálisis de energías dispersivas de Rayos X.

  5. Hemostatic findings of pleural fluid in dogs and the association between pleural effusions and primary hyperfibrino(geno)lysis: A cohort study of 99 dogs.

    Science.gov (United States)

    Zoia, Andrea; Drigo, Michele; Piek, Christine J; Simioni, Paolo; Caldin, Marco

    2018-01-01

    The primary objective of this study was to determine if activation of coagulation and fibrinolysis occurs in canine pleural effusions. Thirty-three dogs with pleural effusions of different origin were studied. Pleural effusion fibrinogen concentrations were significantly lower, while pleural fibrin-fibrinogen degradation products (FDPs) and D-dimer concentrations were significantly higher than those in plasma (P dogs with pleural effusion, and whether the presence of a concurrent inflammatory process may have activated the hemostatic cascade, with its intrinsically linked secondary hyperfibrinolysis, masking the concurrent PHF. The previously 33 selected dogs with pleural effusion (group 1) were compared to two control groups of 33 healthy (group 2) and 33 sick dogs without pleural effusion (group 3). Serum fibrinogen, FDPs, D-dimer, C-reactive protein (CRP), fibrinogen/CRP ratio, and frequency of PHF were determined. Fibrinogen, FDPs, D-dimer and CRP concentrations in group 1 were significantly increased compared to group 2 (P dogs with pleural effusion compared to healthy dogs. Nevertheless, the decrease in the fibrinogen/CRP ratio in group 1 compared to group 3, considering the higher FDPs and similar D-dimer concentrations, would suggest that PHF is also more frequent in dogs with pleural effusion compared to sick control dogs, and that this phenomenon is hidden due to concurrent secondary hyperfibrinolysis.

  6. ESTUDIO MULTICÉNTRICO LONGITUDINAL DE DEPLECIÓN DE LINFOCITOS B EN LUPUS ERITEMATOSO SISTÉMICO REFRACTARIO: ESTUDIO LESIMAB

    OpenAIRE

    Nieves-Martín, Laura

    2014-01-01

    Cohorte multicéntrica retrospectiva de pacientes con LES refractario a terapia estándar que fueron tratados con rituximab. Estudio de efectividad y seguridad tras uno o varios cursos de tratamiento. Así mismo análisis lipídico básico de los pacientes antes y después del tratamiento.

  7. Genómica Funcional de Plantas: Estudio del Desarrollo de Flores y Frutos Genómica Funcional de Plantas: Estudio del Desarrollo de Flores y Frutos

    Directory of Open Access Journals (Sweden)

    Octavio Rubén Salazar Moya

    2012-02-01

    Full Text Available La última fase del desarrollo floral es la fertilización de los óvulos y la formación de los frutos,que son muy importantes tanto biológica como económicamente. Notoriamente, más del 80% de los alimentos que son consumidos por el ser humano proviene de flores y frutos.La obtención de conocimientos acerca de las bases moleculares del desarrollo de frutos en especies modelo es de gran interés científico, y un paso indispensable para poder facilitar investigaciones y de ser factible, aplicaciones en frutos de consumo humano. Especialmente en un país como México, con tal riqueza en la diversidad de frutos, este tipo de estudios es necesario y científicamente muy interesante, y tiene repercusiones económicas potenciales importantes. La meta del laboratorio es descubrir genes nuevos involucrados en el desarrollo de frutos, empleando los recursos que brindan plantas modelo como Arabidopsisthaliana. Se hace un enfoque especial en genes que afectan la identidad celular, morfología y que causan partenocarpia (frutos carentes de semillas, para más tarde estudiarlos enotras especies y hacer ensayos para conocer sus alcances en dichas especies.The last stages of fl oral development are ovule fertilization and fruit formation. Fruits are very important both biologically and economically. Notably, more than 80% of human food is obtained from flowers and fruits. Gathering basic knowledge about the molecular mechanisms of fruit development from model species is of great scientific interest, and is an essential step to facilitate research and, when feasible, applications in fruits consumed by humans. Especially in countries like Mexico, which has such a great diversity of fruits, this kind of research is both necessary and scientifically interesting, and has potentially important economic repercussions. The goal of the lab is to discover new genes that are involved inflower development, making use of the resources provided by model plants like

  8. Hemostatic findings of pleural fluid in dogs and the association between pleural effusions and primary hyperfibrino(geno)lysis: A cohort study of 99 dogs

    Science.gov (United States)

    Drigo, Michele; Piek, Christine J.; Simioni, Paolo; Caldin, Marco

    2018-01-01

    The primary objective of this study was to determine if activation of coagulation and fibrinolysis occurs in canine pleural effusions. Thirty-three dogs with pleural effusions of different origin were studied. Pleural effusion fibrinogen concentrations were significantly lower, while pleural fibrin-fibrinogen degradation products (FDPs) and D-dimer concentrations were significantly higher than those in plasma (P pleural fluids, there is evidence of coagulation activation and fibrinolysis. The secondary aims of the current study were to determine if primary hyperfibrinolysis ([PHF] i.e., elevated plasma FDPs with a normal D-dimer concentrations), occurs in dogs with pleural effusion, and whether the presence of a concurrent inflammatory process may have activated the hemostatic cascade, with its intrinsically linked secondary hyperfibrinolysis, masking the concurrent PHF. The previously 33 selected dogs with pleural effusion (group 1) were compared to two control groups of 33 healthy (group 2) and 33 sick dogs without pleural effusion (group 3). Serum fibrinogen, FDPs, D-dimer, C-reactive protein (CRP), fibrinogen/CRP ratio, and frequency of PHF were determined. Fibrinogen, FDPs, D-dimer and CRP concentrations in group 1 were significantly increased compared to group 2 (P pleural effusion compared to healthy dogs. Nevertheless, the decrease in the fibrinogen/CRP ratio in group 1 compared to group 3, considering the higher FDPs and similar D-dimer concentrations, would suggest that PHF is also more frequent in dogs with pleural effusion compared to sick control dogs, and that this phenomenon is hidden due to concurrent secondary hyperfibrinolysis. PMID:29462172

  9. Resiliencia en estudiantes universitarios. Un estudio comparado entre carreras

    Directory of Open Access Journals (Sweden)

    Juan Francisco Caldera Montes

    2016-07-01

    Full Text Available El presente trabajo tuvo como propósito conocer el nivel de resiliencia en una muestra de estudiantes universitarios de una institución de educación superior pública del estado de Jalisco, México. Además de ello, se analizó la relación y diferencia entre la resiliencia y las variables de sexo, edad y nivel socioeconómico. Para medir la variable de interés se utilizó la Escala de Resiliencia desarrollada por Wagnild y Young (1993 en su versión traducida y adaptada al español por Heilemann, Lee y Kury (2003, la cual está constituida por 25 reactivos en escalamiento tipo Likert. Los resultados muestran niveles altos de resiliencia en los estudiantes universitarios, no encontrándose diferencias significativas entre las variables carrera, sexo y edad de los estudiantes. Se concluye que la resiliencia alta es una característica muy generalizada en la mayoría de los estudiantes universitarios. Finalmente, se sugiere la ampliación de la línea de investigación con otros tamaños muestrales y otro tipo de carreras, así como la ejecución de trabajos que relacionen a la resiliencia con otras variables psicosociales no contempladas en el estudio. Abstract This paper aimed to know the level of resilience from a sample of university students in a public institution of education in the state of Jalisco, México. In addition to that, the relationship and difference between resilience and the variables of gender, age and socioeconomic status were analyzed. For this research, it was used the Resilience Scale developed by Wagnild and Young (1993 in the version translated and adapted to Spanish by Heilemann, Kury and Lee (2003, which consists of 25 reagents in the Likert scaling. This was used to measure the variable of interest. The results show high levels of resilience in university students and there were no significant differences between the career, sex and age variables. It was concluded that high resilience is a very generalized

  10. Estudio observacional de habilidades quirúrgicas en residentes

    Directory of Open Access Journals (Sweden)

    Eduardo B. Arribalzaga

    Full Text Available Introducción: existen actualmente "agujeros negros" en la transmisión de conocimientos aplicables que obligan a poner atención en los mecanismos de adquisición de destrezas y habilidades. El objetivo es evaluar características cualitativas de las habilidades en 2 tipos de procedimientos técnicos básicos al alcance de un cirujano general. Material y Métodos: estudio preliminar exploratorio observacional prospectivo en un Hospital Universitario consistente en la observación de los pasos de una toracotomía axilar o una dermolipectomía abdominal anterior con neo ombligo. Se analizó la práctica de las operaciones programadas, no discriminando que médicos residentes las habían efectuado. Los datos se medían en una lista de cotejos con una escala tipo Likert modificada de 3 items. Cada paso de la técnica observada era evaluado sin considerar el tiempo de duración de cada maniobra. Los evaluadores eran ajenos al equipo quirúrgico actuante ubicados donde no se los veía (en miradores para evitar sesgos o maniobras que afectaran la actividad. Se usaron pruebas estadísticas de ANOVA y prueba de t para detectar validez y diferencias en los resultados. Resultados: observada una toracotomía, todos los pasos quirúrgicos fueron evaluados por encima del valor 2 (bueno, sin variaciones en el desarrollo de cada paso ni existir diferencias significativas en la comparación de destrezas. Similares resultados se hallaron en la observación de la otra técnica. Conclusiones: con un sencillo método de observación directa inadvertida por el evaluado con criterios específicos se hizo un diagnóstico de situación demostrando la existencia de habilidades adquiridas en cirujanos en formación durante programas de residencia.

  11. Estudio demográfico de pseudophoenix sargentii

    Directory of Open Access Journals (Sweden)

    1992-01-01

    Full Text Available ETUDE DEMOGRAPHIQUE DE PSEUDOPHOENIX SARGENTII. Une étude démographique est presentée pour six populations du palmier Pseudophoenix sargentii de la presqu‘île du Yucatán au Mexique. La structure des populations a été analysée pour chacune grâce aux modèles de survie, de mortalité et de croissance. Pour déterminer le taux de croissance des populations une analyse matricielle a été utilisée. Une analyse d’élasticité a également été réalisée en vue de déterminer quels stades du cycle biologique avaient le plus fort impact sur le taux de croissance des populations. Les résultats montrent qu‘il existe une variabilité spatiale assez importante du comportement des populations. Se presenta un estudio demográfico de seis poblaciones de la palma Pseudophoenix sargentii en la Península de Yucatán, México. Se analiza la estructura de las poblaciones de acuerdo a los patrones de sobrevivencia, mortalidad y crecimiento de cada una. Se realizan simulaciones matriciales para determinar la tasa de crecimiento de las poblaciones, y un análisis de elasticidad con objeto de determinar los estadios del ciclo de vida con mayor influencia en la tasa de crecimiento poblacional. Los resultados muestran que existe gran variabilidad espacial en el comportamiento de las poblaciones. DEMOGRAPHIC STUDY OF PSEUDOPHOENIX SARGENTII. A demographic study of six populations of the palm Pseudophoenix sargentii in the Yucatan Peninsula, Mexico, is presented. The population structure is analyzed and interpreted in terms of survival, mortality and growth patterns of each phenological stage into which the populations were divided. A matrix population analysis was conducted to determine the populations’ finite growth rates, and elasticity analysis was used to determine the relative contribution of each stage to the population growth rate. The results show a great deal of spatial variability among populations.

  12. Vólvulo del sigmoides: Morbilidad; estudio de 63 pacientes

    Directory of Open Access Journals (Sweden)

    Eddy Sierra Enrique

    1998-04-01

    Full Text Available Se realiza un estudio de 63 pacientes que presentaron obstrucción intestinal por vólvulo del sigmoides, a los cuales se les trató por diferentes métodos en el Hospital Yekatit 12, de Addis Abeba, Etiopía, durante el período de agosto de 1988 a julio de 1990. Esta afección se presentó en el 87,3 % de los pacientes con más de 50 años y es la causa más frecuente de obstrucción intestinal en dicha región, pues representa el 75,0 % de la serie estudiada. Se trataron médicamente de urgencia por medio de la reducción no quirúrgica del vólvulo del sigmoides un total de 12 pacientes, para el 19,0 % de la serie; en el 75,0 % de ellos se obtuvo la reducción del vólvulo, mientras que en el 25,0 % restante se fracasó. Los resultados del tratamiento quirúrgico de urgencia por la técnica de la desvolvulación, mostraron una recurrencia del 55,5 %. La mortalidad por tratamiento médico es cero, y por tratamiento quirúrgico es 13,7 %. Dentro de las distintas formas de tratamiento quirúrgico, el de resección y anastomosis primaria presenta el 23,8 % de mortalidadA study of 63 patients who suffered from intestinal obstruction due to sigmoid volvulus is conducted. They were treated by different methods at the Yekatit 12 Hospital, in Addis Abeba, Ethiopia, from August, 1988, to July, 1990. This affection was present in 87,3 % of the patients over 50 and it is the most frequent cause of intestinal obstruction in that region, since it accounts for 75.0 % of the series studied. 12 patients were urgently treated by means of the non-surgical reduction of the sigmoid volvulus, accounting for 19.0 %. Volvulus reduction was possible in 75 % of them, where as it failed in 25 %. The results of the urgent surgical treatment by the removal of the volvulus showed a recurrence of 55.5 %. The mortality from medical treatment was null, whereas the mortality from surgical treatment was 13.7 %. Among the different forms of surgical treatment, resection and

  13. Estudio comparativo de seis dominios científicos nacionales

    Directory of Open Access Journals (Sweden)

    Espinosa-Calvo, M.ª Eugenia

    2009-09-01

    Full Text Available This work analyses the area of materials science. Materials science has existed since the earliest civilizations. The development and evolution of societies has been intimately linked to the ability of their members to produce and shape materials to meet their needs. However, materials science has only recently been established as a recognised academic discipline, a consequence of the agglomeration of different areas of science. The citation habits and scientograms associated with this field could therefore be related to the scientific tradition and culture of each country. In this study, the citation networks of six of the most developed countries in the year 2002 were examined using a new technique that graphically represents these networks as scientograms. The technique makes use of cocitation between topic categories of the JCR to generate a network of categories which is graphically represented. The scientograms obtained, which represent the citation networks of each country examined, reflect the cultures of the different nations studied.

    En este trabajo se analiza la clase Ciencia de los Materiales presente desde las civilizaciones más antiguas ya que el desarrollo y la evolución de las sociedades siempre han estado íntimamente vinculados a la capacidad que han tenido sus miembros para producir y conformar los materiales necesarios para satisfacer sus necesidades, pero como disciplina se ha establecido de manera muy reciente por la aglomeración de distintas categorías científicas pertenecientes a otras áreas. Por los que los hábitos de citación y los scientogramas podrían estar relacionados con la tradición y cultura de la ciencia existente en cada país en el año estudiado. Para realizar el estudio hemos utilizado las redes de citación de seis de los países más desarrollados en el año 2002, utilizando una técnica novedosa que las representa gráficamente mediante unos gráficos denominados scientogramas. Esta t

  14. Methodology for electrical studies in industrial networks including the study of electric arc; Metodologia para los estudios electricos en redes industriales incluyendo el estudio de arco electrico

    Energy Technology Data Exchange (ETDEWEB)

    Rasgado Casique, Jose Pepe; Silva Farias, Jose Luis [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)]. E-mail: jrasgado@iie.org.mx; jlsilva@iie.org.mx

    2010-11-15

    This article presents a methodology for conducting electrical studies in industrial networks. The methodology included the study of arc flash as a very important area of current basic electrical studies, such as power flow, short circuit and coordination. The aim of this study is to determine the Personal Protective Equipment (PPE) and flash protection boundary for personnel working with or near energized equipment, based on the IEEE Std 1584-2004 and NFPA-70E- 2004. Also included are criteria and recommendations to reduce incident energy level (cal/cm{sup 2}). At work we used a distribution network for industrial type test. The studies were carried out using a commercial program for the analysis of electrical networks. [Spanish] En este articulo se presenta una metodologia para llevar a cabo los estudios electricos en redes industriales. En la metodologia se incluye al estudio de arco electrico como un area muy importante de los estudios electricos basicos actuales, como: flujos de potencia, cortocircuito y coordinacion de protecciones. El objetivo de dicho estudio es determinar el Equipo de Proteccion Personal (EPP) apropiado y los limites de proteccion para el personal que opera con o cerca de equipo energizado, con base en las normas IEEE Std. 1584-2004 y la NFPA-70E-2004. Ademas, se incluyen criterios y recomendaciones para disminuir el nivel de energia incidente (cal/cm{sup 2}). En el trabajo se utilizo una red de distribucion tipo industrial de prueba. Los estudios se llevaron a cabo utilizando un programa comercial para el analisis de redes electricas.

  15. Estudio de la viabilidad en la impresión en 3D de piezas poliméricas para elevadores

    OpenAIRE

    HARO MARTÍ, ALEJANDRO

    2016-01-01

    [ES] Se realizara el estudio de la viabilidad del uso de la impresión 3D en la sustitución de determinadas piezas utilizadas en instalaciones elevadoras. El estudio abarca desde la selección de la pieza polimérica a sustituir. Estudio de los esfuerzos a los que se ve implicada. Estudio del material empleado y su posible sustitución. Estudio del empleo del proceso de impresión 3D en los posibles materiales disponibles. Estudio económico. Haro Martí, A. (2016). Estudio de la viabilidad en la...

  16. Inactivation of the Autolysis-Related Genes lrgB and yycI in Staphylococcus aureus Increases Cell Lysis-Dependent eDNA Release and Enhances Biofilm Development In Vitro and In Vivo.

    Directory of Open Access Journals (Sweden)

    Cristiana Ossaille Beltrame

    Full Text Available Staphylococcus aureus ica-independent biofilms are multifactorial in nature, and various bacterial proteins have been associated with biofilm development, including fibronectin-binding proteins A and B, protein A, surface protein SasG, proteases, and some autolysins. The role of extracellular DNA (eDNA has also been demonstrated in some S. aureus biofilms. Here, we constructed a Tn551 library, and the screening identified two genes that affected biofilm formation, lrgB and yycI. The repressive effect of both genes on the development of biofilm was also confirmed in knockout strains constructed by allelic recombination. In contrast, the superexpression of either lrgB or yycI by a cadmium-inducible promoter led to a decrease in biofilm accumulation. Indeed, a significant increase in the cell-lysis dependent eDNA release was detected when lrgB or yycI were inactivated, explaining the enhanced biofilm formed by these mutants. In fact, lrgB and yycI genes belong to distinct operons that repress bacterial autolysis through very different mechanisms. LrgB is associated with the synthesis of phage holin/anti-holin analogues, while YycI participates in the activation/repression of the two-component system YycGF (WalKR. Our in vivo data suggest that autolysins activation lead to increased bacterial virulence in the foreign body animal model since a higher number of attached cells was recovered from the implanted catheters inoculated with lrgB or yycI knockout mutants.

  17. The solubilisation of boar sperm membranes by different detergents - a microscopic, MALDI-TOF MS, 31P NMR and PAGE study on membrane lysis, extraction efficiency, lipid and protein composition

    Directory of Open Access Journals (Sweden)

    Müller Karin

    2009-11-01

    Full Text Available Abstract Background Detergents are often used to isolate proteins, lipids as well as "detergent-resistant membrane domains" (DRMs from cells. Different detergents affect different membrane structures according to their physico-chemical properties. However, the effects of different detergents on membrane lysis of boar spermatozoa and the lipid composition of DRMs prepared from the affected sperm membranes have not been investigated so far. Results Spermatozoa were treated with the selected detergents Pluronic F-127, sodium cholate, CHAPS, Tween 20, Triton X-100 and Brij 96V. Different patterns of membrane disintegration were observed by light and electron microscopy. In accordance with microscopic data, different amounts of lipids and proteins were released from the cells by the different detergents. The biochemical methods to assay the phosphorus and cholesterol contents as well as 31P NMR to determine the phospholipids were not influenced by the presence of detergents since comparable amounts of lipids were detected in the organic extracts from whole cell suspensions after exposure to each detergent. However, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry applied to identify phospholipids was essentially disturbed by the presence of detergents which exerted particular suppression effects on signal intensities. After separation of the membrane fractions released by detergents on a sucrose gradient only Triton X-100 and sodium cholate produced sharp turbid DRM bands. Only membrane solubilisation by Triton X-100 leads to an enrichment of cholesterol, sphingomyelin, phosphatidylinositol and phosphatidylethanolamine in a visible DRM band accompanied by a selective accumulation of proteins. Conclusion The boar sperm membranes are solubilised to a different extent by the used detergents. Particularly, the very unique DRMs isolated after Triton X-100 exposure are interesting candidates for further studies regarding the

  18. Bleeding and infection with external ventricular drainage: a systematic review in comparison with adjudicated adverse events in the ongoing Clot Lysis Evaluating Accelerated Resolution of Intraventricular Hemorrhage Phase III (CLEAR-III IHV) trial.

    Science.gov (United States)

    Dey, Mahua; Stadnik, Agnieszka; Riad, Fady; Zhang, Lingjiao; McBee, Nichol; Kase, Carlos; Carhuapoma, J Ricardo; Ram, Malathi; Lane, Karen; Ostapkovich, Noeleen; Aldrich, Francois; Aldrich, Charlene; Jallo, Jack; Butcher, Ken; Snider, Ryan; Hanley, Daniel; Ziai, Wendy; Awad, Issam A

    2015-03-01

    Retrospective series report varied rates of bleeding and infection with external ventricular drainage (EVD). There have been no prospective studies of these risks with systematic surveillance, threshold definitions, or independent adjudication. To analyze the rate of complications in the ongoing Clot Lysis: Evaluating Accelerated Resolution of Intraventricular Hemorrhage Phase III (CLEAR III) trial, providing a comparison with a systematic review of complications of EVD in the literature. Patients were prospectively enrolled in the CLEAR III trial after placement of an EVD for obstructive intraventricular hemorrhage and randomized to receive recombinant tissue-type plasminogen activator or placebo. We counted any detected new hemorrhage (catheter tract hemorrhage or any other distant hemorrhage) on computed tomography scan within 30 days from the randomization. Meta-analysis of published series of EVD placement was compiled with STATA software. Growing or unstable hemorrhage was reported as a cause of exclusion from the trial in 74 of 5707 cases (1.3%) screened for CLEAR III. The first 250 patients enrolled have completed adjudication of adverse events. Forty-two subjects (16.8%) experienced ≥1 new bleeds or expansions, and 6 of 250 subjects (2.4%) suffered symptomatic hemorrhages. Eleven cases (4.4%) had culture-proven bacterial meningitis or ventriculitis. Risks of bleeding and infection in the ongoing CLEAR III trial are comparable to those previously reported in EVD case series. In the present study, rates of new bleeds and bacterial meningitis/ventriculitis are very low despite multiple daily injections, blood in the ventricles, the use of thrombolysis in half the cases, and generalization to >60 trial sites.

  19. Estudios de impacto ambiental y sus tendencias en Colombia

    Directory of Open Access Journals (Sweden)

    Hernández Luis Alfredo

    1994-12-01

    Full Text Available

    Un Estudio de Impacto Ambiental (ElA constituye una herramienta de planificación, ordenamiento y toma de decisiones de las acciones del hombre y/o la naturaleza para preservar o mantener una oferta de bienes naturales para el desarrollo sostenible de la sociedad. El EIA busca identificar, describir, evaluar y controlar los efectos que las acciones del hombre tengan sobre el medio, incluyendo al hombre como el agente principal que induce cambios sobre el ambiente. Antes de 1993 los EIA se caracterizaban por la existencia de una formulación ambiental aceptable en términos de los estudios ecológicos (Artículos 27 y 28 del Decreto Ley 2811 de 1974, 220 dentro del marco de la Ley 23 de 1973 o código de los recursos naturales, no constituían elementos decisorios para proyecto u obras de desarrollo, y eran muy descriptivos, extensos y poco analíticos. El componente socioeconómico no tenía ninguna relación con los elementos de la naturaleza. Además, algunos El A se hacían posteriormente a la ejecución de un proyecto, además de constituir un requisito más dentro de la documentación exigida, no existían consulta ni responsabilidad a nivel de las autoridades del orden territorial, no se consultaba ni educaba a la comunidad sobre los alcances en el espacio y en el tiempo de proyectos u obras. Finalmente, carecían de planes de seguimiento, monitoreo y control. Y no se establecían la reposición o compensación ambiental ni los costos ambientales tenían repercusión en la relación costo-beneficio del proyecto. Actualmente, los EIA tienen connotaciones bien diferentes. Desde el punto de vista institucional, la

  20. Estudio del comportamiento térmico del polvo de aluminio estabilizado con el yeso

    Directory of Open Access Journals (Sweden)

    López-Delgado, A.

    2005-12-01

    Full Text Available The aluminium dust is a hazardous waste from the secondary aluminium remelting industry. A stabilization/solidification process was developed at CENIM with the aim of decreasing its reactivity. Gypsum was used as a binder material. This work summarizes the study of the thermal behavior of the aluminium dust stabilized with gypsum, in order to establish the influence of the residue in the reactions of hydration/dehydration of the gypsum during the hardening process, namely: partial dehydration of calcium sulphate dihydrate, dehydration of calcium sulphate hemi-hydrate, dehydration of aluminium hydroxide and anhydrite soluble/anhydrite insoluble transformation. The activation energy associated to all those reactions was determined by Differential Scanning Calorimetry.

    El polvo de aluminio (pAl es un residuo peligroso procedente de la metalurgia secundaría del aluminio. Con objeto de reducir la reactividad del mismo, en el CENIM se ha desarrollado un proceso de estabilización/solidificación mediante su fraguado con yeso. En este trabajo se presenta el estudio del comportamiento térmico del pAl estabilizado con el yeso, con el fin de establecer la influencia del residuo en las reacciones de hidratación/deshidratación del yeso durante el proceso del fraguado, a saber: deshidratación parcial del sulfato cálcico dihidrato, deshidratación del hemihidrato, deshidratación del hidróxido de aluminio y transformación de anhidrita soluble en anhidrita insoluble. Asimismo se ha estudiado la cinética asociada a cada una de las reacciones anteriormente mencionadas mediante Calorimetría Diferencial de Barrido.

  1. Los estudios de la audiencia: una visión crítica desde la economía política

    Directory of Open Access Journals (Sweden)

    José A. Vela Montero

    2001-01-01

    Full Text Available El autor expone y analiza desde una perspectiva diacrónica la evolución de los estudios culturales desde su nacimiento hasta la actualidad. En especial, este estudio descriptivo se centra en el proceso y las consecuencias del abandono del análisis sociológico marxista y, en concreto, de los postulados de la economía política. Un abandono que, en la práctica, tiene como consecuencia la incapacidad de los estudios culturales para llevar a cabo su objetivo original y fundamental: la transformación social. ABSTRACTThis article analyses the development of the cultural studies from its birth until present time. Especially, this descriptive study is focus in the process and the consequences of the abandonment of the sociological Marxist analysis and, in short, of the postulates of the political economy. An abandonment that, in the practice, it has as consequence the inability of the cultural studies to carry out their original and fundamental objective: the social transformation.

  2. Los estudios culturales y la construcción social del patrimonio cultural

    Directory of Open Access Journals (Sweden)

    Rosa Elena Malavassi Aguilar

    2017-01-01

    Full Text Available El presente artículo forma parte una investigación más amplia, cuyo tema es la construcción social del patrimonio urbano y arquitectónico en la ciudad de San José, Costa Rica. El proyecto tiene por objetivo analizar la forma en que se ha construido el concepto de patrimonio en Costa Rica, específicamente en la ciudad de San José, su capital. En este texto se realiza una revisión de los postulados de los principales autores de la corriente de los estudios culturales, para definir un esquema de análisis aplicable al caso de estudio. El artículo se estructura en cuatro apartados: inicia con la obra de los pioneros de este tipo de estudios en Inglaterra, luego se analiza su repercusión en otras latitudes, por ejemplo, el desarrollo de los estudios poscoloniales en lugares como la India, para luego pasar a los trabajos de autores latinoamericanos, y finalmente hacer referencia a su impacto en el desarrollo de los estudios culturales en Costa Rica.

  3. Motivación y Hábitos de estudio en estudiantes de la Universidad Alas Peruanas, Filial Juliaca

    OpenAIRE

    Quispe Tapia, David

    2017-01-01

    El objetivo de la presente tesis es sustentar la influencia de la motivación en el habito de estudio en los estudiantes de la Universidad Alas Peruana, filial Juliaca. Asimismo, conocer de qué manera influye la motivación intrínseca y la motivación extrínseca en los hábitos de estudio. Los métodos y el estudio corresponde al tipo cualitativo, básico y con un diseño descriptivo correlacional, con una población de estudio de 160 estudiantes de los cuales se obtuvo una muestra ...

  4. Management of Pediatric Tumor Lysis Syndrome

    African Journals Online (AJOL)

    Urate oxidase in prevention and treatment of hyperuricemia associated with lymphoid malignancies. Leukemia. 1997. Nov;11(11):1813-6. 43. Elitek [package insert]. Bridgewater, NJ: Sanofi-. Aventis US LLC; 2009. 44. Pui CH, Mahmoud HH, Wiley JM, Woods GM,. Leverger G, Camitta B, Hastings C, Blaney SM, Relling.

  5. Estudio y caracterización de vidriados vitrocerámicos basados en piroxeno

    Directory of Open Access Journals (Sweden)

    Lucas, F.

    2004-10-01

    Full Text Available A method was proposed to develop pyroxene-based glass-ceramic glazes. First, was studied, by X-ray diffraction and scanning electron microscopy, the effect of several additives in the monophasic crystallization of pyroxene from glasses in the CaO•MgO•Al2O3•SiO2 quaternary system. After, it was determined the sinterization intervals and thermal properties of the glasses, by hot stage microscopy and dilatometry. Finally, some studied glasses were chosen and glazed tiles were developed under fast firing wall- and floor-tile industrial cycles. The results proved the reproducibility of the microstructural characteristics obtained in the previous study with glasses. The measurement of different mechanical properties confirmed their potential application in nowadays industrial processing.

    En este trabajo se presenta una metodología para desarrollar vidriados vitrocerámicos basados en piroxeno. En primer lugar se ha estudiado, mediante difracción de rayos X y microscopía electrónica de barrido, el efecto de diferentes aditivos en la cristalización monofásica de piroxeno a partir de vidrios en el sistema CaO•MgO•Al2O3•SiO2. Posteriormente, se han determinado los intervalos de sinterización y características térmicas de los vidrios, mediante microscopía de calefacción y dilatometría. Finalmente, con los vidrios seleccionados se han preparado piezas esmaltadas mediante tratamientos térmicos utilizados habitualmente en la industria de pavimento y revestimiento, comprobando la reproducibilidad de las características microestructurales obtenidas en los estudios iniciales en vidrios. Se han medido sobre las piezas esmaltadas diferentes propiedades que permiten confirmar su potencial aplicación en procesados industriales de monococción y/o bicocción.

  6. LOS ESTUDIOS DE MERCADO EN APOYO A LA EXPORTACIÓN DE SERVICIOS PROFESIONALES: ESTUDIO DE CASO DE LA INVERSIONES GAMMA S/A

    Directory of Open Access Journals (Sweden)

    Mercedes Sánchez-Sánchez

    2016-01-01

    Full Text Available El trabajo refiere la importancia de los estudios de mercado (EM en la toma de decisiones en las organizaciones. Dichos estudios caracterizan el entorno: competitivo, económico, social, regulador, político etc., y determinan las amenazas y las oportunidades a que se enfrenta la organización, para comercializar sus productos y servicios. Se presenta un estudio de caso que refleja los resultados alcanzados por la aplicación de los EM por la empresa cubana Inversiones Gamma S/A, comercializadora de servicios profesionales del Ministerio de Ciencia, Tecnología y Medio Ambiente de Cuba (CITMA, en la identificación de socios comerciales y en la concertación de alianzas estratégicas, con empresas que brindan servicios medioambientales y de riesgo industrial para las industrias petroleras: PEMEX (México, PDVSA (Venezuela y Petroamazonas (Ecuador. Así como también con empresas que realizan el servicio de restauración de playas en: Cancún, Bahamas y Jamaica.

  7. Estudio inmobiliario de Torrejón de Ardoz

    Directory of Open Access Journals (Sweden)

    Fernando Roch Peña

    1999-07-01

    This paper -presented as document to help in the Torrejón city centre planning process- describes the circumstances in which the property developer machine works in a township of the metropolitan area of Madrid, as well as its relation with the processes of social differentiation in this context ruled by the self owned housing. This study shows how property developer activity changes spaces created with social homogeneous into new "good quality spaces" intentionally self excluded, whilst the number of empty houses increases and old 60's and 70's districts decline threatening to become social exclusion areas. New "good quality spaces" intentionally self excluded appear into spaces which were in its origin socially homogeneous, meanwhile the amount of empty houses increases and the old districts of the 60's and 70's decline threatening become areas of social exclusion. The need of correcting these processes opens a specific opportunity for the local urban policy through planning and management.

  8. Envejecimiento satisfactorio e indicadores de fragilidad en los mayores de la comunidad. Estudio Octabaix

    OpenAIRE

    Ferrer, Assumpta; Formiga, Francesc; Sanz, Héctor; Monserrate, Elena; Verges, Dolors

    2014-01-01

    El envejecimiento satisfactorio como estado óptimo de un proceso de adaptación es poco conocido en las personas más mayores. Objetivo: Describir envejecimiento satisfactorio y analizar su asociación con indicadores de fragilidad en personas de 86 años. Diseño: Estudio descriptivo transversal al segundo año de seguimiento de un ensayo clínico (estudio Octabaix). Emplazamiento: Siete centros de atención primaria. Participantes: Personas nacidas en 1924, no institucionalizadas. Me...

  9. Estudio experimental del comportamiento del hormigón confinado sometido a compresión

    OpenAIRE

    Aire Untiveros, Carlos Máximo

    2002-01-01

    La tesis presenta los resultados de un extenso estudio experimental de probetas cilíndricas de hormigón sometidas a confinamiento lateral cargadas axialmente. En el estudio se consideró el confinamiento activo y pasivo. El confinamiento activo consistió en aplicar una presión hidrostática en una célula triaxial y el confinamiento pasivo fue mediante tubos de acero rellenos de hormigón y probetas de hormigón zunchadas con polímeros reforzados con fibras (FRP) de carbono y vidrio. Se ensayaron ...

  10. Estudio teórico de formas inusuales y modificadas de los ácidos nucleicos

    OpenAIRE

    Faustino Pló, Ignacio

    2013-01-01

    1) Estudio teórico de nucleobases modificadas. Las modificaciones químicas de ácidos nucleicos tienen una amplia variedad de aplicaciones tanto en clínica, utilizadas como agentes antisentido, así como en el estudio de las estructuras de los propios ácidos nucleicos, las interacciones proteína DNA o en la catálisis de ácidos nucleicos por poner algunos ejemplos. Actualmente se sintetizan cientos de análogos de nucleósidos estándar en laboratorios farmacéuticos, algunos de ellos como, ...

  11. Estudio de caso: estrategias internacionales aplicadas a la empresa Astro Maquinaria Ltda.

    OpenAIRE

    Araujo Peñate, Ana Maria; Piza Mora, Juan David; Velasco Zamora, Maria Ximena

    2013-01-01

    En el estudio de caso se podrá encontrar información relacionada a la empresa ASTRO MAQUINARIA Ltda. la cual lleva más de 20 años en el mercado, generando soluciones a las necesidades de maquinaria y equipos, especialmente en el área de bombeo. Desde el principio la empresa ha estado importando desde Estados Unidos el portafolio de productos que ofrecen, actualmente tienen la representación comercial de la empresa Pentair Pump Group. Este estudio de caso pretende identificar cual es la estrat...

  12. Estudio de los metabolitos secundarios exudados por las hojas de Aloe barbadensis

    OpenAIRE

    López González, Germán

    2016-01-01

    Debido a las numerosas propiedades terapéuticas Aloe Barbadensis, como cicatrizantes, hidratantes digestivas o anti-inflamatorias; se plantea el estudio, aislamiento y determinación estructural de los componentes de su exudado. Este se obtiene al hacer un corte en la hoja, y contiene principalmente antraquinonas y glicósidos de antronas, algunos de ellos con actividad anti-cancerígena reportada. Se realizará también un estudio biodirigido del mismo y se utilizarán técnicas como la resonancia ...

  13. Estudio de eficiencia de una acería en Sestao

    OpenAIRE

    Pindado Cebrián, Javier

    2014-01-01

    El objetivo final del proyecto es la realización de un estudio que permita optimizar la eficiencia de una instalación de aire comprimido, instalada en una planta de producción de acero, reduciendo de este modo, el consumo energético de la misma. La instalación objeto de estudio cuenta con dos salas independientes que generan el caudal demandado por la instalación. Una de ellas, la que más caudal genera, consta de seis compresores, mientras que la otra está equipada con cuatro máquinas. ...

  14. Estudio del balance energético en velocistas de alto rendimiento

    OpenAIRE

    Lorente Gutiérrez, Jesús

    2015-01-01

    El objetivo de este estudio fue evaluar el balance energético en tres atletas de alto rendimiento durante 28 días, que coincidieron con el periodo competitivo de pista cubierta. La ingesta energética fue estudiada a partir de registros alimentarios durante los 28 días. Del mismo modo, el gasto energético fue estimado por tres métodos, mediante registros de actividad durante 28 días, mediante el estudio del ritmo metabólico basal estudiado por calorimetría indirecta, aplicándole el fa...

  15. La inteligencia emocional en jóvenes universitarios: un estudio descriptivo

    OpenAIRE

    Vera Pedroza, A.; Alejandre Espinosa, M.; Piña Vázquez, D.L.; Segura Hernández, A.

    2016-01-01

    El estudio desarrolla el tema de la inteligencia emocional en estudiantes pertenecientes a la Facultad de Pedagogía de la Universidad Veracruzana, campus Poza Rica-Tuxpan, Veracruz, México; durante el periodo agosto 2015-enero 2016. Siendo el objetivo principal del trabajo determinar el nivel de inteligencia emocional de los alumnos universitarios y su relación en el contexto escolar. La metodología utilizada en el estudio fue de corte cuantitativa-descriptiva, que se caracteriza por recabar ...

  16. Un estudio exploratorio del perfil de las mujeres empresarias en el Perú

    OpenAIRE

    Avolio Alecchi, Beatrice

    2008-01-01

    La investigación identifica el perfil de las mujeres empresarias en el Perú mediante la exploración cualitativa de sus características demográficas; sus antecedentes educativos, laborales y familiares; sus habilidades administrativas; la naturaleza de sus empresas; los factores que las han estimulado a convertirse en empresarias; y los obstáculos para el desarrollo de sus empresas. El estudio utiliza el paradigma cualitativo basado en estudios de caso de veinticuatro mujeres empresarias en el...

  17. Relaciones entre variables de personalidad y manifestaciones del estrés laboral: un estudio exploratorio

    OpenAIRE

    Herrero García, Alicia

    2012-01-01

    El presente trabajo explora la forma de interactuar de algunas manifestaciones de estrés laboral y diferentes características de la personalidad. Se pretende analizar la relevancia de las variables de personalidad y los efectos que éstas pueden tener sobre los trastornos derivados del estrés laboral, es decir, el papel de la personalidad como moderadora de dichos trastornos. Para realizar el estudio se entregó una batería de cuestionarios a 50 sujetos participantes. El estudio cuenta con una ...

  18. Percentiles de salto con contramovimiento en escolares de Bogotá, Colombia: Estudio FUPRECOL

    OpenAIRE

    Ferro Vargas, Martha

    2016-01-01

    Objetivo: Determinar la distribución por percentiles de salto con contramovimiento (CMJ) en una población escolar de Bogotá, Colombia, perteneciente al estudio Fuprecol. Métodos: Estudio transversal realizado entre 2846 niños y 2754 adolescentes, entre 9 a 17 años de edad, pertenecientes a 18 instituciones educativas oficiales de Bogotá, Colombia. Se evaluó el CMJ, de acuerdo, con lo establecido por la batería de condición física, Fuprecol. Se calcularon, los percentiles (P3, P...

  19. GORDURA, DISCRIMINACIÓN Y CLASISMO: UN ESTUDIO EN JÓVENES DE SANTIAGO DE CHILE

    OpenAIRE

    María Alejandra Energici Sprovera; Elaine Acosta Gonzáles; Florencia Borquez Grancelli; Macarena Huaiquimilla Paredes

    2017-01-01

    Resumen El estudio de la obesidad desde la psicología social se ha realizado principalmente desde una aproximación cognitivo conductual omitiendo los contextos sociales en que se realizan juicios discriminadores. Con el objetivo de comprender los significados con que se construye la gordura y su interacción con otras formas de exclusión social, hemos realizado un estudio cualitativo de jóvenes de Santiago de Chile. Trabajamos con tres grupos de discusión, que analizamos siguiendo las directri...

  20. Estudio sobre las variables que intervienen en el abandono físico o negligencia infantil

    OpenAIRE

    Moreno Manso, Juan Manuel

    2002-01-01

    La escasez de estudios en materia de abandono físico o negligencia determinan un desconocimiento bastante importante de la tipología de maltrato infantil, considerada hoy por hoy como la de mayor incidencia, tanto a través de estudios nacionales como internacionales. Por ello, a través del análisis de diecinueve variables individuales, sociales, relacionales y familiares, pretendemos aportar un mayor conocimiento sobre una práctica de desprotección infantil con...