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Sample records for lycopersicon esculentum cultivar

  1. Performance de six cultivars de tomates Lycopersicon esculentum ...

    African Journals Online (AJOL)

    ... de déterminer sur la parcelle après apport d\\'insecticides, les incidences du TYLCV ... others diseases: the yellow leaf curl, bacterial wilt and root knot nematodes. In order to identify performants cultivars, six improved cultivars of tomato were ...

  2. Performance de six cultivars de tomates Lycopersicon esculentum ...

    African Journals Online (AJOL)

    Administrateur

    Elle s'est déroulée sur deux cycles de production, l'un en saison sèche et ... respectively. The results obtained allowed us to make a hierarchical classification of the cultivars according to their ..... Plan directeur du développement agricole ...

  3. Plant Spacing and Cultivar Affects Yield Components, Qualitative Traits and Early Ripening of Tomato (Lycopersicon esculentum

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    Ebrahim MAMNOIE

    2013-12-01

    Full Text Available Two-year field trial was set up on sandy clay soil in the Jiroft and Kahnouj Agricultural Research Center with the objective to determine the effect of plant spacing and different cultivars on the yield and qualitative characteristics of tomato (Lycopersicon esculentum. This experiment was performed as split plot based on complete randomized block design with 3 replications. The main plots were in – row spacing in 4 levels include 0.3, 0.4, 0.5 and 0.6 m and the subplots were cultivars naming ‘Shef ’, ‘Peto Early CH’ and ‘FDT 202’. The results showed that, fruit length to diameter ratio, total acidity, fruit number per plant, mean fruit weight, yield per plant, total yield and first harvest to total harvests ratio were significantly affected by plant spacing. In addition, cultivars showed significant effect on all traits evaluated (p<0.01. In this experiment, fruit length to diameter ratio and total acidity increased as plant spacing increased, however it had no effect on total soluble solids. In this study, total fruit yield is being increased while the yield per plant, number of fruit per plant and fruit weight is being reduced by increased number of plants per unit area. Although among tomato cultivar, ‘Peto Early CH’ had a higher yield over other cultivars, but cultivar ‘Shef ’ showed higher yield in the first harvest. Generally it seems according to the results collected that plant spacing 0.3 m and ‘Shef ’ cultivar owing to better adaptation and higher commercial yield for production in Jiroft city is suggested.

  4. Evaluation of volatile metabolites as markers in Lycopersicon esculentum L. cultivars discrimination by multivariate analysis of headspace solid phase microextraction and mass spectrometry data.

    Science.gov (United States)

    Figueira, José; Câmara, Hugo; Pereira, Jorge; Câmara, José S

    2014-02-15

    To gain insights on the effects of cultivar on the volatile metabolomic expression of different tomato (Lycopersicon esculentum L.) cultivars--Plum, Campari, Grape, Cherry and Regional, cultivated under similar edafoclimatic conditions, and to identify the most discriminate volatile marker metabolites related to the cultivar, the chromatographic profiles resulting from headspace solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-qMS) analysis, combined with multivariate analysis were investigated. The data set composed by the 77 volatile metabolites identified in the target tomato cultivars, 5 of which (2,2,6-trimethylcyclohexanone, 2-methyl-6-methyleneoctan-2-ol, 4-octadecyl-morpholine, (Z)-methyl-3-hexenoate and 3-octanone) are reported for the first time in tomato volatile metabolomic composition, was evaluated by chemometrics. Firstly, principal component analysis was carried out in order to visualise data trends and clusters, and then, linear discriminant analysis in order to detect the set of volatile metabolites able to differentiate groups according to tomato cultivars. The results obtained revealed a perfect discrimination between the different Lycopersicon esculentum L. cultivars considered. The assignment success rate was 100% in classification and 80% in prediction ability by using "leave-one-out" cross-validation procedure. The volatile profile was able to differentiate all five cultivars and revealed complex interactions between them including the participation in the same biosynthetic pathway. The volatile metabolomic platform for tomato samples obtained by HS-SPME/GC-qMS here described, and the interrelationship detected among the volatile metabolites can be used as a roadmap for biotechnological applications, namely to improve tomato aroma and their acceptance in the final consumer, and for traceability studies. Copyright © 2013 Elsevier Ltd. All rights reserved.

  5. Investigating the Tolerance of Tomato (Lycopersicon esculentum Cultivars to Broomrape (Orobanche aegyptiaca pers in Khorassan Razavi

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    M. Zafarian,

    2014-03-01

    Full Text Available To investigate the tolerance of tomato cultivars to Egyptian broomrape (Orobanche aegyptiaca pers., an experiment was conducted in a randomized complete block design with 11 treatments and 3 replications in mazrae Nemoune Astan Quds Razavi in Mashhad, Iran, 2012. Treatment were 11 varieties (Peto early CH, Sterling (Karon, Khorram, Petorak, DNP 3005, PS 6515, SPEEDY, IDEN, VADI STAR, FIRINZEH and DNP 3001 which were transplanted in the field along with broomrape. Sampling was done at two stages: 1- after appearance and establishment of broomrape on tomato root where the dry weight, stem number and node numbers of broomrape on tomato root and tomato dry weight were measured and 2- at the end of growing season where tomato fruit weight and its yield were determined. Result indicated that Sterling and Khorram cultivars did have the least broomrape dry weight, stem number and node numbers of broomrape on tomato root and while produced highest plant dry weight, fruit and yield as compared to the other cultivars. Thus, may be considered as tolerant cultivars. Petorak and DNP 3001 on the other hand, presented the most broomrape dry weight, stem number and node number on tomato root. However, Petorak, Peto early CH and FIRINZEH cultivars produced the least plant weight, fruit and yield and thus, they can be called the sensitive cultivars. DNP 3001 being highly attacked by broomrape produced increased fruit yield and therefore compensated its ill effects.

  6. Soluble Proteins, Proline, Carbohydrates and Na+/K+ Changes in Two Tomato (Lycopersicon esculentum Mill. Cultivars under in vitro Salt Stress

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    Fariba Amini

    2005-01-01

    Full Text Available The effect of NaCl stress on soluble proteins, proline, carbohydrates and Na+/K+ of two tomato (Lycopersicon esculentum Mill. cultivars Isfahani and Shirazy were studied. Seeds were germinated on medium containing only water agar, then transferred to MS medium supplemented with different concentrations of NaCl (0, 40, 80, 120 and 160 mM for 21 days. Increasing of salinity resulted in increasing of soluble proteins in stem & leaf of cv. Isfahani but decreasing in cv. Shirazy. Soluble proteins in roots of both cultivars showed some variations. When concentration of NaCl in the medium was increased proline contents of stem-leaf and roots in both cultivars increased significantly. However, cv. Shirazy showed higher amount of proline level. Proline content in stem-leaf in comparing with roots was higher in two cultivars. In response to increasing of salt concentration of the medium, the average amount of total carbohydrate in stem-leaf of cv. Shirazy increased but, in cv. Isfahani level of carbohydrate decreased. When explants form both cultivars were exposed to the higher concentration of salt the level of carbohydrate in roots increased. High-level salinity decreased the reduced sugars in both cv. either in stem-leaf or roots. Salt stress increased Na+ and decreased K+ content in both cultivars significantly.

  7. Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation

    Science.gov (United States)

    Muntean, Cristina M.; Leopold, Nicolae; Tripon, Carmen; Coste, Ana; Halmagyi, Adela

    2015-06-01

    In this work the surface-enhanced Raman scattering (SERS) spectra of five genomic DNAs from non-cryopreserved control tomato plants (Lycopersicon esculentum Mill. cultivars Siriana, Darsirius, Kristin, Pontica and Capriciu) respectively, have been analyzed in the wavenumber range 400-1800 cm-1. Structural changes induced in genomic DNAs upon cryopreservation were discussed in detail for four of the above mentioned tomato cultivars. The surface-enhanced Raman vibrational modes for each of these cases, spectroscopic band assignments and structural interpretations of genomic DNAs are reported. We have found, that DNA isolated from Siriana cultivar leaf tissues suffers the weakest structural changes upon cryogenic storage of tomato shoot apices. On the contrary, genomic DNA extracted from Pontica cultivar is the most responsive system to cryopreservation process. Particularly, both C2‧-endo-anti and C3'-endo-anti conformations have been detected. As a general observation, the wavenumber range 1511-1652 cm-1, being due to dA, dG and dT residues seems to be influenced by cryopreservation process. These changes could reflect unstacking of DNA bases. However, not significant structural changes of genomic DNAs from Siriana, Darsirius and Kristin have been found upon cryopreservation process of tomato cultivars. Based on this work, specific plant DNA-ligand interactions or accurate local structure of DNA in the proximity of a metallic surface, might be further investigated using surface-enhanced Raman spectroscopy.

  8. Genetic diversity analysis based on molecular marker and quantitative traits of the response of different tomato (Lycopersicon esculentum Mill. cultivars to drought stress

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    Metwali Ehab M.R.

    2016-01-01

    Full Text Available The drought tolerance of tomato (Lycopersicon esculentum Mill. is a trait needing urgent improvement due to recent climate changes and limited water availability. We therefore conducted a greenhouse screening experiment to identify tomato cultivars with improved drought tolerance. Several sensitivity and tolerance indices were computed based on morphological markers. With the aim of establishing a correlation to these markers, a total of 16 inter-simple sequence repeat (ISSR primers were used, the genetic diversity among cultivars was elucidated and clustering the cultivars into groups based on their molecular profiles was performed. The obtained results indicated that selection indices, such as geometric mean productivity (GMP, mean productivity (MP, tolerance index (TOL,and stress tolerance index (STI, represented suitable indices for screening the drought tolerance of tomato cultivars. An interesting correlation of the ISSR analyses to these morphological findings was established according to 83 detectable fragments derived from 10 primers. The highest value of the effective multiplex ratio (EMR and marker index (MI was detected for primer INC7 followed by INC1. Based on Jaccard's similarity coefficients, the genetic distance of the genotypes varied from 0.702 to 0.942 with a mean value of 0.882. The results showed a clear-cut separation of the 15 tomato cultivars due to their genetic variability, making them a valuable genetic source for their incorporation into potential breeding programs. Molecular data were in good agreement with the results as regards selection indices, and both of them will be useful tools for improvement of the tomato germplasm.

  9. Folate content in tomato ( Lycopersicon esculentum ). influence of cultivar, ripeness, year of harvest, and pasteurization and storage temperatures.

    Science.gov (United States)

    Iniesta, M Dolores; Pérez-Conesa, Darío; García-Alonso, Javier; Ros, Gaspar; Periago, M Jesús

    2009-06-10

    The effects of cultivar, on-vine ripening, and year of harvest on the folate content of raw tomatoes were studied. Folate content in hot-break tomato puree (HTP) subjected to pasteurization at different temperatures and its evolution during the shelf life of tomato juice were also investigated. 5-Methyltetrahydrofolate (5-CH(3)-H(4)-folate) was the only folate compound identified in raw tomatoes and HTP, but tetrahydrofolate (H(4)-folate) was 10% of the folate detected in tomato juice. The content of folates in raw tomatoes ranged from 4.1 to 35.3 microg/100 g of fresh weight and was highly influenced by all of the factors studied. No clear trend of folate content with ripening stage was observed. The extractability of 5-CH(3)-H(4)-folate from HTP increased significantly after pasteurization at 98 degrees C for 40 s, but higher temperatures decreased its content. Tomato juice showed folate losses during storage independent of the storage temperature. Folate losses were higher when tomato juice was packed in glass bottles than in Tetra Pak.

  10. Isolation and characterisation of somatic hybrids between Lycopersicon esculentum and Lycopersicon peruvianum.

    NARCIS (Netherlands)

    Wijbrandi, J.

    1989-01-01

    Several desirable traits, such as disease resistances, have been introduced from more or less related wild Lycopersicon species, into the cultivated tomato, L.esculentum , by classical breeding techniques. Somatic hybridisation by protoplast fusion can enhance the germplasm pool available for tomato

  11. Análisis de la heterosis y de la habilidad combinatoria entre diferentes cultivares de tomate, Lycopersicon esculentum Mill. a partir de un cruzamiento dialélico

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    Vallejo Cabrera Franco Alirio

    1989-06-01

    Full Text Available Se realizó el análisis de la heterosis y de la habilidad combinatoria entre 21 cultivares de tomate, para el carácter producción por planta y sus componentes a partir de un cruzamiento dialélico (6 progenitores, 15 híbridos F1, sin incluir los recíprocos: utilizando el método experimental 2, modelo I propuesto por Griffing (1956. Se encontraron diferencias altamente significativas para los efectos de hcg y de hce para todos los caracteres a excepción del carácter producción de frutos de primera (frutos de más de 180 g. Los híbridos Motelle x Ángela I 5100, Motelle x Zambao y Licapal 21 x Zambao presentaron altos efectos de h c e para el carácter producción por planta. Los anteriores efectos asociados con altas heterobeltiosis hacen que estos híbridos sean promisorios para una posterior comercialización.An analysis of heterosis and combining ability of traits related with per plant production was carried out using a diallele crossing between different tomate cultivars, Lycopersicon esculentum Mill (six parent and 15 F1 hybrids from as possible crossing in one direction, according to the methodology proposed by Griffing (1956, selecting experimental method 2, and model 1. In the genetic variation of all traits related to per plant production, except in first class fruit trait, the general combining ability (gen non addite action effects participate jointly and in highly significant manner. Hybrids Motelle x Angela I 5100, Motelle x Zambao y Licapal21 x Zambao, exhibited high s c a effects for the trait per plant production. Former effects related to high heterobeltyosis make these hybrids promising for later marketing.

  12. Capítulo XI: análisis de la heterosis y de la habilidad combinatoria entre diferentes cultivares de tomate, Lycopersicon esculentum, Mill. a partir de un cruzamiento dialélico

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    Sanint P. Rodrigo

    1994-12-01

    Full Text Available

    An analysis of heterosis and combining ability of traits related with per plant production was carried out using a diallel crossing between different tomato cultivars, Lycopersicon esculentum Mill (six parents and 15 F, hybrids from all possible crossing in one direction, according to the methodology proposed by Griffing (1956, selecting experimental method 2, and model 1. In the genetic variation of all traits related to per plant production, the general combining ability (addite gene action and specific combining abilyty (non-aditive gene action effects participate jointly and in high1y significant manner. Hybrids Motelle x Angela I 5200, Motelle x Zarnbao y Licapal 21 x Zarnbao, exhibited high s.c.a. effects for the trait per plant production. Former effects related to high heterosis make these hybrids promising for later marketing.

    Se analizó la heterosis y la habilidad combinatoria para el carácter producción por planta y sus componentes, a partir de un cruzamiento dialélico (6 progenitores, 15 híbridos F1 sin incluir los recíprocos utilizando el método experimental 2, modelo 1 propuesto por Griffing (1956. Se encontraron diferencias altamente significativas para los efectos de h.c.g. y de h.c.e. para todos los caracteres estudiados. Los híbridos Motelle x Angela I 5100, Motelle x Zambao y Licapal-21 x Zambao presentaron altos efectos de h.c.e. para el carácter producción por planta. Los anteriores efectos asociados con altas heteroheltiosis hacen que estos híbridos sean promisorios para posterior comercialización.

  13. Allelopathic potential of Asarum europaeum toward Lycopersicon esculentum

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    Monica MARIAN

    2011-05-01

    Full Text Available Asarum europaeum L. contains water-soluble substances which manifest allelopathic potential. Aqueous extracts from leaves and stems of Asarum europaeum were assayed to determine their allelopathic effects on Lycopersicon esculentum and Zea mays seeds germination and early seedling growth. The germination of the investigated seeds was found to be inhibited with increasing of the Asarum europaeum L. extract concentration. Moreover, the active substances extracted from leaves were found to be more inhibitory on the seeds germination in comparison with those extracted from stems.

  14. The Effects of Planting Distances and Different Stages of Maturity on the Quality of Three Cultivars of Tomatoes (Lycopersicon esculentum Mill

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    Atefeh TABASI

    2013-08-01

    Full Text Available In this investigation the effects of cultivar, row spacing and different stages of maturity on qualitative characteristics of tomato (ascorbic acid, total soluble solids (TSS, β-carotene and lycopene have been evaluated. Experiment was performed by factorial analysis with 3 replicates in completely randomized design (CRD. First treatment was three cultivars of tomato, second treatment was four planting distances and third treatment was different stages of maturity. The results showed that all treatments had significant influence on the levels of ascorbic acid, soluble solids, β-carotene and lycopene. Generally, wider spacing and deep red fruits had the highest quality. Therefore, choosing appropriate cultivars, special planting distances and suitable stage of maturity can increase fruit quality of tomato.

  15. Three-year comparative study of polyphenol contents and antioxidant capacities in fruits of tomato (Lycopersicon esculentum Mill.) cultivars grown under organic and conventional conditions.

    Science.gov (United States)

    Anton, Dea; Matt, Darja; Pedastsaar, Priit; Bender, Ingrid; Kazimierczak, Renata; Roasto, Mati; Kaart, Tanel; Luik, Anne; Püssa, Tõnu

    2014-06-04

    In the present study, four tomato cultivars were grown under organic and conventional conditions in separate unheated greenhouses in three consecutive years. The objective was to assess the influence of the cultivation system on the content of individual polyphenols, total phenolics, and antioxidant capacity of tomatoes. The fruits were analyzed for total phenolic content by the Folin-Ciocalteau method and antioxidant capacity by the DPPH free radical scavenging assay. Individual phenolic compounds were analyzed using HPLC-DAD-MS/MS. Among 30 identified and quantified polyphenols, significantly higher contents of apigenin acetylhexoside, caffeic acid hexoside I, and phloretin dihexoside were found in all organic samples. The content of polyphenols was more dependent on year and cultivar than on cultivation conditions. Generally, the cultivation system had minor impact on polyphenols content, and only a few compounds were influenced by the mode of cultivation in all tested cultivars during all three years.

  16. Características fisiológicas de microtomateiros fitocromo-mutantes Physiological characteristics of micro-tomato (Lycopersicon esculentum P. Miller) phytochrome-mutants

    National Research Council Canada - National Science Library

    Hyrandir Cabral de Melo; Evaristo Mauro de Castro; Ângela Maria Soares; Cynthia de Oliveira; Sílvio Júnio Ramos

    2009-01-01

    ... (Lycopersicon esculentum P. Miller cv. Micro-Tom) fitocromo-mutantes. A cultivar Micro-Tom e os mutantes aurea (deficiente na biossíntese do cromóforo dos fitocromos), atroviolacea (atv) e high pigment1 (hp1...

  17. Tomato bushy stunt virus (TBSV) infecting Lycopersicon esculentum.

    Science.gov (United States)

    Hafez, El Sayed E; Saber, Ghada A; Fattouh, Faiza A

    2010-01-01

    Tomato bushy stunt virus (TBSV) was detected in tomato crop (Lycopersicon esculentum) in Egypt with characteristic mosaic leaf deformation, stunting, and bushy growth symptoms. TBSV infection was confirmed serologically by ELISA and calculated incidence was 25.5%. Basic physicochemical properties of a purified TBSV Egh isolate were identical to known properties of tombusviruses of isometric 30-nm diameter particles, 41-kDa coat protein and the genome of approximately 4800 nt. This is the first TBSV isolate reported in Egypt. Cloning and partial sequencing of the isolate showed that it is more closely related to TBSV-P and TBSV-Ch than TBSV-Nf and TBSV-S strains of the virus. However, it is distinct from the above strains and could be a new strain of the virus which further confirms the genetic diversity of tombusviruses.

  18. Tomato ( Lycopersicon esculentum ) seeds: new flavonols and cytotoxic effect.

    Science.gov (United States)

    Ferreres, Federico; Taveira, Marcos; Pereira, David M; Valentão, Patrícia; Andrade, Paula B

    2010-03-10

    In this study, seeds of Lycopersicon esculentum Mill. were analyzed by HPLC/UV-PAD/MS(n)-ESI. Fourteen flavonoids were identified, including quercetin, kaempferol, and isorhamnetin derivatives, with 13 of them being reported for the first time in tomato seeds. The major identified compounds were quercetin-3-O-sophoroside, kaempferol-3-O-sophoroside, and isorhamnetin-3-O-sophoroside. A significant cell proliferation inhibition (>80%), against rat basophile leukemia (RBL-2H3) cell line, was observed with this extract (IC(50) = 5980 microg/mL). For acetylcholinesterase inhibitory activity, a concentration-dependent effect was verified (IC(20) = 2400 microg/mL). The same behavior was noted regarding antioxidant capacity, evaluated against DPPH (IC(10) = 284 microg/mL), nitric oxide (IC(25) = 396 microg/L), and superoxide radicals (IC(25) = 3 microg/mL).

  19. Genetics of Hybrid Incompatibility Between Lycopersicon esculentum and L. hirsutum

    Science.gov (United States)

    Moyle, Leonie C.; Graham, Elaine B.

    2005-01-01

    We examined the genetics of hybrid incompatibility between two closely related diploid hermaphroditic plant species. Using a set of near-isogenic lines (NILs) representing 85% of the genome of the wild species Lycopersicon hirsutum (Solanum habrochaites) in the genetic background of the cultivated tomato L. esculentum (S. lycopersicum), we found that hybrid pollen and seed infertility are each based on 5–11 QTL that individually reduce hybrid fitness by 36–90%. Seed infertility QTL act additively or recessively, consistent with findings in other systems where incompatibility loci have largely been recessive. Genetic lengths of introgressed chromosomal segments explain little of the variation for hybrid incompatibility among NILs, arguing against an infinitesimal model of hybrid incompatibility and reinforcing our inference of a limited number of discrete incompatibility factors between these species. In addition, male (pollen) and other (seed) incompatibility factors are roughly comparable in number. The latter two findings contrast strongly with data from Drosophila where hybrid incompatibility can be highly polygenic and complex, and male sterility evolves substantially faster than female sterility or hybrid inviability. The observed differences between Lycopersicon and Drosophila might be due to differences in sex determination system, reproductive and mating biology, and/or the prevalence of sexual interactions such as sexual selection. PMID:15466436

  20. A major QTL introgressed from wild Lycopersicon hirsutum confers chilling tolerance to cultivated tomato (Lycopersicon esculentum).

    Science.gov (United States)

    John Goodstal, F; Kohler, Glenn R; Randall, Leslie B; Bloom, Arnold J; St Clair, Dina A

    2005-09-01

    Many plants of tropical or subtropical origin, such as tomato, suffer damage under chilling temperatures (under 10 degrees C but above 0 degrees C). An earlier study identified several quantitative trait loci (QTLs) for shoot turgor maintenance (stm) under root chilling in an interspecific backcross population derived from crossing chilling-susceptible cultivated tomato (Lycopersicon esculentum) and chilling-tolerant wild L. hirsutum. The QTL with the greatest phenotypic effect on stm was located in a 28 cM region on chromosome 9 (designated stm 9), and enhanced chilling-tolerance was conferred by the presence of the Lycopersicon hirsutum allele at this QTL. Here, near-isogenic lines (NILs) were used to verify the effect of stm 9, and recombinant sub-NILs were used to fine map its position. Replicated experiments were performed with NILs and sub-NILs in a refrigerated hydroponic tank in the greenhouse. Sub-NIL data was analyzed using least square means separations, marker-genotype mean t-tests, and composite interval mapping. A dominant QTL controlling shoot turgor maintenance under root chilling was confirmed on chromosome 9 using both NILs and sub-NILs. Furthermore, sub-NILs permitted localization of stm 9 to a 2.7 cM interval within the original 28 cM QTL region. If the presence of the L. hirsutum allele at stm 9 also confers chilling-tolerance in L. esculentum plants grown under field conditions, it has the potential to expand the geographic areas in which cultivated tomato can be grown for commercial production.

  1. Tomato ( Lycopersicon esculentum prevents lead-induced testicular toxicity

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    Emmanuel O Salawu

    2009-01-01

    Full Text Available Background: Lead, an example of heavy metals, has, for decades, being known for its adverse effects on various body organs and systems such that their functions are compromised. Aim: In the present study, the ability of lead to adversely affect the male reproductive system was investigated and tomato ( Lycopersicon esculentum : Source of antioxidants paste (TP was administered orally to prevent the adverse effects of Pb. Materials and Methods: Fifteen Sprague Dawley rats, randomised into three groups (n = 5, were used for this study. Animals in Group A served as the control and were drinking distilled water. Animals in Groups B and C were drinking 1% Pb (II acetate (LA. Group C animals were, in addition to drinking LA, treated with 1.5 ml of TP/day. All treatments were for 8 weeks. Statistical Analysis Used: A Mann-Whitney U -test was used to analyse the results obtained. Results: The obtained results showed that Pb caused a significant reduction in the testicular weight, sperm count, life-death ratio, sperm motility, normal sperm morphology, and plasma and tissue superoxide dismutase and catalase activity, but a significant increase in plasma and tissue malondialdehyde concentration. But, Pb did not cause any significant change in the serum testosterone level. TP, however, significantly reduced these adverse effects of Pb. Conclusion: These findings lead to the conclusion that TP significantly lowered the adverse effects of Pb exposure on the kidney as well as Pb-induced oxidative stress.

  2. Lycopersicon esculentum seeds: an industrial byproduct as an antimicrobial agent.

    Science.gov (United States)

    Taveira, Marcos; Silva, Luís R; Vale-Silva, Luís A; Pinto, Eugénia; Valentão, Patrícia; Ferreres, Federico; Guedes de Pinho, Paula; Andrade, Paula B

    2010-09-08

    Lycopersicon esculentum (tomato) fruit is a widely studied matrix. However, only few works focus their attention on its seeds, which constitute a major byproduct of the tomato processing industry. In this study the antimicrobial potential of ten different tomato seed extracts from "Bull's heart" and "Cherry" varieties were analyzed against Gram-positive (Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus luteus, Enterococcus faecalis and Bacillus cereus) and Gram-negative (Proteus mirabilis, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium) bacteria and fungi (Candida albicans, Aspergillus fumigatus and Trichophyton rubrum). Regarding antibacterial capacity, the different extracts were revealed to be active only against Gram-positive bacteria, E. faecalis being the most susceptible one (MIC: 2.5-10 mg/mL). Concerning antifungal activity, "Bull's heart" extracts were the most active. In a general way C. albicans was the most susceptible species (MIC: 5-10 mg/mL). The chemical composition of the extracts was also pursued, concerning organic acids, phenolics and fatty acids, in order to establish a possible relationship with the observed antimicrobial effect.

  3. Effect of textile waste water on tomato plant, Lycopersicon esculentum.

    Science.gov (United States)

    Marwari, Richa; Khan, T I

    2012-09-01

    In this study Sanganer town, Jaipur was selected as study area. The plants of Lycopersicon esculentum var. K 21(Tomato) treated with 20 and 30% textile wastewater were analyzed for metal accumulation, growth and biochemical parameters at per, peak and post flowering stages. Findings of the study revealed that chlorophyll content was most severely affected with the increase in metal concentration. Total chlorophyll content showed a reduction of 72.44% while carbohydrate, protein and nitrogen content showed a reduction of 46.83, 71.65 and 71.65% respectively. With the increase in waste water treatment the root and shoot length, root and shoot dry weight and total dry weight were reduced to 50.55, 52.06, 69.93, 72.42, 72.10% respectively. After crop harvesting, the fruit samples of the plants treated with highest concentration of textile waste water contained 2.570 mg g(-1)d.wt. of Zn, 0.800 mg g(-1) d.wt. Cu, 1.520 mg g(-1) d.wt. Cr and 2.010 mg g(-1) d.wt. Pb.

  4. Morphogenetic Potential of Tomato (Lycopersicon esculentum cv. ‘Arka Ahuti’ to Plant Growth Regulators

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    Kanakapura K. NAMITHA

    2013-05-01

    Full Text Available A highly reproducible in vitro regeneration method for tomato (Lycopersicon esculentum Mill. cultivar ‘Arka Ahuti’ was established by using hypocotyl, leaf and cotyledon explants from in vitro raised seedlings on Murashige and Skoog medium supplemented with different concentrations and combinations of hormones 6-Benzylamino purine (2 to 4 mg/L and Indole-3-acetic acid (0.1 to 1 mg/L. The medium supplemented with 2 mg/L 6-benzylamino purine and 0.1 mg/L indole-3-acetic acid was found to be the best for inducing direct shoot regeneration and multiple shoots per explant from hypocotyl explants. Callus induction was observed in all the explants and regeneration of shoots was also promoted by all these combinations. Shoots were transferred to the elongation medium which also induced 100% rooting. After hardening, plants were transferred to soil. Thus, a tissue culture base line was established for ‘Arka Ahuti’ cultivar of tomato for obtaining direct regeneration using hypocotyl, leaf and cotyledon as explants.

  5. Shoot regeneration from GUS-transformed tomato (Lycopersicon esculentum) hairy root.

    Science.gov (United States)

    Moghaieb, Reda E A; Saneoka, Hirofumi; Fujita, Kounosuke

    2004-01-01

    To study the influence of genetic background on the transformation and regeneration of cultivated tomato plants, hairy root lines of tomato (Lycopersicon esculentum) were obtained by inoculating the hypocotyl explants of three tomato cultivars with the Agrobacterium rhizogenes strain DCAR-2, which harbors the pBI-121 binary vector. The Ri-T-DNA transformation into the plant DNA was confirmed by both of mikimopine and GUS assay analyses. The regeneration efficiency from hairy root explants was assessed. The data indicated that white embryonic calli were formed within two weeks in the presence of 2 mgl(-1) 2, 4-D plus 0.25 mgl(-1) kinetin. Adventitious shoots emerged from the embryonic callus in the presence of 1 mgl(-1) GA3 along with 0.5 mgl(-1) NAA. The regeneration frequency was higher in the cultivar UC-97, followed by Momotaro and then Edkawi. Molecular confirmation of the integration of the GUS gene into the hairy root-derived plants genomes was done via PCR using GUS-specific primers and also using Southern blotting analysis. Our data shows that regeneration is possible from hairy roots of the cultivated tomato and this system could be used to produce transgenic tomato plants expressing the genes present in Agrobacterium rhizogenes binary vectors.

  6. Desenvolvimento de protocolo para microenxertia do tomateiro Lycopersicon esculentum Mill = Developmental of protocol for micrograffiting to tomato Lycopersicon esculentum Mill

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    Luciano Façanha Marques

    2010-01-01

    Full Text Available A murcha bacteriana, causada por Ralstonia solanacearum, é a principal doença vascular de plantas em todo o mundo. O agente causal é uma bactéria que ocorre em todas as regiões do Brasil, predominando em condições de umidade e temperatura elevadas, fatores propícios aodesenvolvimento do patógeno. Este agente causal é de difícil controle, pois sobrevive nos mais diversos hospedeiros, especialmente Solanaceas, além de outras famílias de importância alimentar, condimentar e medicinal. O uso de espécies do gênero Solanum na enxertia convencional e minienxertia em tomateiro Lycopersicon esculentum é limitada em função da incompatibilidade. Neste trabalho, utilizou-se o método da microenxertia para obtenção de plantas resistentes a doenças como a murcha bacteriana, tendo como cavalo a espécie Solanum palinacanthum Dun. Utilizaram-se dois métodos de microenxertia: convencional em T-invertido e corte em bisel. Foram testados meios de culturas para execução das duas práticas demicropropagação; para isso, realizou-se experimento em diferentes condições ambientais, com presença e ausência de luminosidade e diferentes tratamentos correspondentes a teores de açúcares e sais, submetidos a cinco avaliações com intervalos semanais. Melhor resultado foi encontrado quando se utilizou microenxertia em T-invertido e meio de cultura constituído de sais de MS1/8 de força acrescido de 30 g L-1 de sacarose.The bacterial wilt, caused by Ralstonia solanacearum, is the most important vascular disease among plants in the world. The causal agent is a strain of bacteria found in all regions of Brazil, mainly in places with high humidity and temperature. This bacterium is difficult to control with chemical products, being able to survive in diverse hosts, especially in species of Solanaceae as well as other families of great food, condimental, or medicinal importance. The use of species of the genus Solanum has been attempted, but the

  7. Attenuated Lead Induced Apoptosis in Rat Hepatocytes in the Presence of Lycopersicon Esculentum

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    Hamidreza Ahmadi Ashtiani

    2016-05-01

    Full Text Available Lead (Pb, has, for decades, being known for its adverse effects on various body organs and systems. In the present study, the damage of Pb on the Liver tissue apoptosis was investigated, and Lycopersicon esculentum as an antioxidants source was administered orally to prevent the adverse effects of Pb. Eighteen Wistar rats, randomized into three groups (n=6, were used for this study. Animals in Group A served as the control and were drinking distilled water. Animals in Groups B and C were drinking 1%Lead acetate (LA. Group C animals were, in addition to drinking LA, treated with 1.5 ml/day of Lycopersicon esculentum. Treatments were for three months. The obtained results showed that lead acetate caused significant reductions in the liver weight, plasma and tissue superoxide dismutase and catalase activity, but a significant increase in plasma and tissue malondialdehyde concentration but Lycopersicon esculentum have an inhibitory effect on LA liver adverse effect. So, it can be concluded that Lycopersicon esculentum have a significant protective effect on liver lead acetate adverse effects as well as, lead acetate -induced oxidative stress.

  8. [Bioaccumulation of cadmium and zinc in tomato (Lycopersicon esculentum L.)].

    Science.gov (United States)

    Sbartai, Hana; Djebar, Med Reda; Sbartai, Ibtissem; Berrabbah, Houria

    2012-09-01

    This work aims at evaluating the accumulation of cadmium (Cd) and zinc (Zn) (trace elements) in the organs of young tomato plants (Lycopersicon esculentum L. var. Rio Grande) and their effects on the rate of chlorophyll and enzyme activities involved in the antioxidant system: catalase (CAT), glutathion-S-transferase (GST) and peroxysase ascorbate (APX). Plants previously grown on a basic nutrient solution were undergoing treatment for 7 days, either by increasing concentrations of CdCl(2) or ZnSO(4) (0, 50, 100, 250, 500 μM) or by the combined concentrations of Cd and Zn (100/50, 100/100, 100/250, 100/500 μM). The results concerning the determination of metals in the various compartments of tomato plants as a function of increasing concentrations of Cd or Zn, suggest a greater accumulation of Cd and Zn in the roots compared to leaves. The combined treatment (Cd/Zn) interferes with the absorption of the two elements according to their concentrations in the culture medium. The presence of Zn at low concentrations (50 μM of Zn/100 μM Cd) has little influence on the accumulation of Cd in the roots and leaves, while the absorption of these two elements in the leaves increases and decreases in roots when their concentrations are equivalent (100/100 μM) compared to treatment alone. When the concentration of Zn is higher than that of Cd (500 μM of Zn/100 μM Cd) absorption of the latter is inhibited in the roots while increasing their translocation to the leaves. Meanwhile, the dosage of chlorophylls shows that they tend to decrease in a dose-dependent for both treatments (Cd or Cd/Zn), however, treatment with low concentrations of Zn (50 and 100 μM) stimulates chlorophyll synthesis. However, treatment with different concentrations of Cd seems to induce the activity of the enzymes studied (CAT, APX, GST). It is the same for treatment with different concentrations of Zn and this particularly for the highest concentrations. Finally, the combined treatment (Zn

  9. Functional Characterization of Mi, a Root-knot Nematode Resistance Gene from Tomato( Lycopersicon esculentum L.)

    Institute of Scientific and Technical Information of China (English)

    Ru-Gang Chen; Li-Ying Zhang; Jun-Hong Zhang; Wei Zhang; Xue Wang; Bo Ouyang; Han-Xia Li; Zhi-Biao Ye

    2006-01-01

    Root-knot nematodes (Meloidogyne spp.) cause major economic damage to numerous crop species around the world. Plant resistance is the most important attribute that is able to suppress invasion by the rootknot nematodes. In the present study, a candidate root-knot nematode resistance gene (Mi) was isolated from the resistant tomato (Lycopersicon esculentum L.) line RN-1. Expression profiling analysis revealed that this gene was expressed specifically in the roots, stems, and leaves, but not in the flowers or fruits.To verify the real function of this candidate gene, both sense and inteference RNA (RNAi) vectors were constructed. We obtained 31 transgenic plants with between one and seven copies of T-DNA inserts of sense Mi from two nematode-susceptible tomato cultivars as assayed by polymerase chain reaction (PCR)and Southern blotting analysis. Reverse transcription-PCR analysis revealed that expression levels of the Mi gene varied in different transgenic plants. Nematode assays showed that the resistance to root-knot nematodes was significantly improved in some transgenic lines compared with untransformed susceptible controls and that the resistance was heritable in selfed progeny. Loss of function via RNAi further confirmed the role of the Mi gene and the original resistant lines became susceptible to root-knot nematodes.

  10. Estudios genéticos básicos para la creación de nuevos cultivares de tomate, Lycopersicon esculentum Mill., adaptados a las condiciones de Colombia: interpretación in6tegral de la investigación

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    Vallejo Cabrera Franco Alirio

    1994-12-01

    Full Text Available

    El Programa de investigación: "Mejoramiento Genético y Producción de Semillas de Hortalizas" se viene desarrollando en la Facultad de Ciencias Agropecuarias de Palmira, desde 1985. Busca crear y seleccionar cultivares (variedades o híbridos adaptados a las condiciones, necesidades y recursos del horticultor colombiano y a la formación de investigadores en la carrera de Agronomía y en sus diferentes Programas de Posgrados, en las áreas de Producción Vegetal, Mejoramiento Genético, Sistemas de Semillas y Suelos y Aguas. El Programa involucra cuatro hortalizas (tomate, pimentón, zapallo y cilantro las cuales se  consideran importantes y/o promisorias para la  agricultura colombiana. No se descarta que, en el futuro, el programa incluya otras especies. En el presente trabajo se entregan los resultados de la investigación relacionados, con la especie tomate, Lycopersicon esculentum Mill.

  11. Infection of Cultured Thin Cell Layer Roots of Lycopersicon esculentum by Meloidogyne incognita

    OpenAIRE

    Radin, D. N.; Eisenback, J. D.

    1991-01-01

    A new aseptic culture system for studying interactions between tomato (Lycopersicon esculentum) and Meloidogyne incognita is described. Epidermal thin cell layer explants from peduncles of tomato produced up to 20 adventitious roots per culture in 4-9 days on Murashige &Scoog medium plus kinetin and indole acetic acid. Rooted cultures were transferred to Gamborg's B-5 medium and inoculated with infective second-stage juveniles. Gall formation was apparent 5 days after inoculation and egg prod...

  12. Induction of volatile organic compound in the leaves of Lycopersicon esculentum by chitosan oligomer

    Institute of Scientific and Technical Information of China (English)

    He Peiqing; Lin Xuezheng; Shen Jihong; Huang Xiaohang; Chen Kaoshan; Li Guangyou

    2005-01-01

    Induction of VOCs (volatile organic compounds) in the leaves of Lycopersicon esculentum by chitosan oligomer elictor was studied. The results demonstrated that VOCs in chitosan oligomer-treated leaves showed stronger inhibitory activity against Botrytis cinerea than that in water-treated leaves, and the spore germination was reduced by 22.1% in 144h after elicitor treatment at a concentration of 1.0%. A total of 16 constituents were detected in water-treated leaves, and chitosan oligomer treatment increased the amount of VOCs production. Chitosan oligomer at different concentration and different time courses of induction treatment could induce different amount of VOCs. Chitosan oligomer resulted in an optimal production of VOCs in 144h after elicitation at concentration of 0.6%. Chitosan oligomer also enhanced activtity of PAL and LOX. The results showed that the enhancement of VOCs production after chitosan oligomer treatment might be an important agent for L.esculentum acquiring resistance against pathogen.

  13. Mannosyl- and Xylosyl-Containing Glycans Promote Tomato (Lycopersicon esculentum Mill.) Fruit Ripening.

    Science.gov (United States)

    Priem, B; Gross, K C

    1992-01-01

    The oligosaccharide glycans mannosylalpha1-6(mannosylalpha1-3)mannosylalpha1-6(mannosylalpha1-3) mannosylbeta1-4-N-acetylglucosamine and mannosylalpha1-6(mannosylalpha1-3)(xylosylbeta1-2) mannosylbeta1-4-N-acetylglucosaminyl(fucosylalpha1-3) N-acetylglucosamine were infiltrated into mature green tomato fruit (Lycopersicon esculentum Mill., cv Rutgers). Coinfiltration of 1 nanogram per gram fresh weight of the glycans with 40 micrograms per gram fresh weight galactose, a level of galactose insufficient to promote ripening, stimulated ripening as measured by red coloration and ethylene production.

  14. Chemical analysis of a polysaccharide of unripe (green) tomato (Lycopersicon esculentum).

    Science.gov (United States)

    Chandra, Krishnendu; Ghosh, Kaushik; Ojha, Arnab K; Islam, Syed S

    2009-11-02

    A polysaccharide (PS-I) isolated from the aqueous extract of the unripe (green) tomatoes (Lycopersicon esculentum) consists of D-galactose, D-methyl galacturonate, D-arabinose, L-arabinose, and L-rhamnose. Structural investigation of the polysaccharide was carried out using total acid hydrolysis, methylation analysis, periodate oxidation study, and NMR studies ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments the structure of the repeating unit of the polysaccharide (PS-I) was established as: [structure: see text].

  15. Nitrogen Requirements for Growth and Early Fruit Development of Drip-Irrigated Processing Tomato (Lycopersicon esculentum Mill.) in Portugal

    Science.gov (United States)

    The effect of continuous application of small quantities of nitrogen (N) in irrigation water and N applied as starter on growth and development of processing tomato (Lycopersicon esculentum Mill.), from transplanting to beginning of fruit set, was studied in two experiments: a pot experiment and a f...

  16. Effects of Lycopersicon esculentum extract on hair growth and alopecia prevention.

    Science.gov (United States)

    Choi, Jae-Suk; Jung, Sung Kyu; Jeon, Min-Hee; Moon, Jin-Nam; Moon, Woi-Sook; Ji, Yi-Hwa; Choi, In Soon; Wook Son, Sang

    2013-01-01

    To evaluate the potential hair growth-promoting activity and the expression of cell growth factors of Lycopersicon esculentum extracts, each 3% (w/w) of ethyl acetate extract (EAE), and supercritical CO2 extract (SCE) of L. esculentum and isolated lycopene Tween 80 solution (LTS) and test hair tonic (THT) containing LTS were applied on the dorsal skin of C57BL/6 mice, once a day for 4 weeks. At week 4, LTS and THT exhibited hair growth-promoting potential similar to that of 3% minoxidil as a positive control (PC). Further, in the LTS group, a significant increase of mRNA expression of vascular endothelial growth factor (VEGF), keratinocyte growth factor, and insulin-like growth factor-1 (IGF-1) was observed than PC, as well as the negative control (NC). In the THT group, increases in IGF-1 and decrease in VEGF and transforming growth factor-β expression were significant over the NC. In a histological examination in the THT group, the induction of anagen stage of hair follicles was faster than that of NC. In the Draize skin irritation study for THT, no observable edema or erythema was observed on all four sectors in the back skin after exposure for 24 or 72 h for any rabbit. Therefore, this study provides reasonable evidence that L. esculentum extracts promote hair growth and suggests that applications could be found in hair loss treatments without skin irritation at moderate doses.

  17. ACTIVIDAD BIOLÓGICA DEL PECTIMORF EN LA MORFOGÉNESIS In Vitro DEL TOMATE (Lycopersicon esculentum, Mill VAR. AMALIA

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    Dagmara Plana

    2003-01-01

    Full Text Available Diferentes investigaciones realizadas en el Laboratorio de Biotecnología de las plantas del Instituto Nacional de Ciencias Agrícolas (INCA, han demostrado el papel de los oligogalacturónidos como biorreguladores del crecimiento y desarrollo in vitro. En este trabajo se estudió la actividad biológica de diferentes concentraciones de la mezcla de oligogalacturónidos conocida como Pectimorf, en la respuesta morfogenética in vitro del tomate (Lycopersicon esculentum, Mill. En los ensayos realizados se emplearon como explantes segmentos apicales de hipocótilos y cotiledones provenientes de plántulas del cultivar Amalia, cultivadas en medio basal complementado con diferentes concentraciones de Pectimorf (1, 5, 10 y 15 mg.L-1 enpresencia o no de bajas concentraciones de BAP (0.25, 0.5 y 1 mg.L-1. Se puso de manifiesto la acción favorable del producto sobre la regeneración in vitro de los explantes estudiados, siendo la dosis de 10 mg.L-1 de Pectimorf la que favoreció la regeneración de plantas a partir de hipocótilos. En el caso de los cotiledones, las mejores combinaciones fueron aquellas donde se emplearon 10 mg.L-1 de Pectimorf con 0.25 y 0.5 mg.L-1 de BAP como complemento del medio de cultivo.

  18. Growth and development of tomato plants Lycopersicon Esculentum Mill. under different saline conditions by fertirrigation with pretreated cheese whey wastewater.

    Science.gov (United States)

    Prazeres, Ana R; Carvalho, Fátima; Rivas, Javier; Patanita, Manuel; Dôres, Jóse

    2013-01-01

    Pretreated cheese whey wastewater (CWW) has been used at different salinity levels: 1.75, 2.22, 3.22, 5.02 and 10.02 dS m(-1) and compared with fresh water (1.44 dS m(-1)). Two cultivars (cv.) of the tomato plant Lycopersicon Esculentum Mill. (Roma and Rio Grande) were exposed to saline conditions for 72 days. Salinity level (treatment) had no significant effects on the fresh weight and dry matter of the leaves, stems and roots. Similar results were found when specific leaf area, leaflet area, ramifications number of 1st order/plant, stem diameter and length, nodes number/stem and primary root length were considered. Conversely, the salinity level significantly influenced the Soil Plant Analysis Development (SPAD) index and the distance between nodes in the plant stem. In the first case, an increase of 21% was obtained in the salinity levels of 5.02 and 10.02 dS m(-1) for cv. Rio Grande, compared with the control run. The results showed that the pretreated CWW can be a source of nutrients for tomato plants, with reduced effects on growth and development.

  19. Control genético del carácter número de frutos por planta y sus componentes en un cruzamiento dialélico entre cultivares de tomate "chonto", Lycopersicon esculentum Mill

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    Urrego P. Jorge E.

    1990-06-01

    Full Text Available Se realizó el análisis genético del carácter número de frutos por planta y sus componentes utilizando un cruzamiento dialélico entre siete cultivares de tomate "chonto" (Ángela Gigante, Licapal- 21, Raminho, Olho Roxo, 1258, 1465, y 1507. El análisis genético-estadístico se efectuó utilizando la metodología desarrollada por Hayman (1954 a, 1954b. No se detectó evidencia de epistasis para ninguno de los caracteres estudiados y los datos experimentales se ajustaron al modelo aditivo-dominante. La acción génica aditiva y no aditiva participaron en forma conjunta y significativa en la expresión de los caracteres número de frutos por planta y número de inflorescencias por planta. Solamente la acción génica no aditiva participó en forma significativa en la expresión del carácter número de frutos por inflorescencia. Se detectó la presencia de sobredominacia en los tres caracteres estudiados. Se pudieron estimar los límites de selección para el carácter número de frutos por planta (34.12 y 3.15 y para el carácter número de frutos por inflorescencia (4.37 y 0.29 para padres completamente dominantes y recesivos, respectivamente. Para el carácter número de inflorescencias por planta, no fue posible estimarlos debido a la baja correlación presentada entre el grado de dominacia y el valor medio de los progenitores.Genetic analysis for fruits per plant, clusters per plant and fruits per cluster characters was carried out using a diallel crossing between seven "chonto" tomato cultivars (Angela Gigante, Licapal- 21, Raminho, Olho Roxo, 1258, 1465 and 1507. Was made a genetic- statistic analysis using the methodology developed by Hayman (1954 a, 1954b. There was no evidence of epistasis in any of characters studied and the experimental data are adjusted to the additive-dominant model. Additive gene action and non-additive gene action participate jointly and highly significant manner, in the fruits per plant, and clusters per

  20. Amino acid sequences of heterotrophic and photosynthetic ferredoxins from the tomato plant (Lycopersicon esculentum Mill.).

    Science.gov (United States)

    Kamide, K; Sakai, H; Aoki, K; Sanada, Y; Wada, K; Green, L S; Yee, B C; Buchanan, B B

    1995-11-01

    Several forms (isoproteins) of ferredoxin in roots, leaves, and green and red pericarps in tomato plants (Lycopersicon esculentum Mill.) were earlier identified on the basis of N-terminal amino acid sequence and chromatographic behavior (Green et al. 1991). In the present study, a large scale preparation made possible determination of the full length amino acid sequence of the two ferredoxins from leaves. The ferredoxins characteristic of fruit and root were sequenced from the amino terminus to the 30th residue or beyond. The leaf ferredoxins were confirmed to be expressed in pericarp of both green and red fruit. The ferredoxins characteristic of fruit and root appeared to be restricted to those tissue. The results extend earlier findings in demonstrating that ferredoxin occurs in the major organs of the tomato plant where it appears to function irrespective of photosynthetic competence.

  1. Infection of Cultured Thin Cell Layer Roots of Lycopersicon esculentum by Meloidogyne incognita.

    Science.gov (United States)

    Radin, D N; Eisenback, J D

    1991-10-01

    A new aseptic culture system for studying interactions between tomato (Lycopersicon esculentum) and Meloidogyne incognita is described. Epidermal thin cell layer explants from peduncles of tomato produced up to 20 adventitious roots per culture in 4-9 days on Murashige &Scoog medium plus kinetin and indole acetic acid. Rooted cultures were transferred to Gamborg's B-5 medium and inoculated with infective second-stage juveniles. Gall formation was apparent 5 days after inoculation and egg production by mature females occurred within 25 days at 25 C in the susceptible genotypes Rutgers and Red Alert. Resistant genotypes LA655, LA656, and LA1022 exhibited a characteristic hypersensitive response. This system provides large numbers of cultured root tips for studies on the molecular basis of the host-parasite relationship.

  2. Acetylcholine causes rooting in leaf explants of in vitro raised tomato (Lycopersicon esculentum Miller) seedlings.

    Science.gov (United States)

    Bamel, Kiran; Gupta, Shrish Chandra; Gupta, Rajendra

    2007-05-30

    The animal neurotransmitter acetylcholine (ACh) induces rooting and promotes secondary root formation in leaf explants of tomato (Lycopersicon esculentum Miller var. Pusa Ruby), cultured in vitro on Murashige and Skoog's medium. The roots originate from the midrib of leaf explants and resemble taproot. ACh at 10(-5) M was found to be the optimum over a wide range of effective concentrations between 10(-7) and 10(-3) M. The breakdown products, choline and acetate were ineffective even at 10(-3) M concentration. ACh appears to have a natural role in tomato rhizogenesis because exogenous application of neostigmine, an inhibitor of ACh hydrolysis, could mimic the effect of ACh. Neostigmine, if applied in combination with ACh, potentiated the ACh effect.

  3. Genetic control of modified genomic region in a firm ripening tomato (Lycopersicon esculentum Mill. mutant

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    Adilson Ricken Schuelter

    2006-01-01

    Full Text Available Studies involving the firm tomato (Lycopersicon esculentum mutant have shown that pleiotropy or geneticlinkage are responsible for modifications in morphological and postharvest traits. The objective of this report was to evaluatethe hypothesis of pleiotropy or genetic linkage linked to morphologic traits and to verify the effect of QTL on fruit firmness.Plants of mutant firm and L. cheesmani were intercrossed; the F2 and F3 generations were analyzed for segregation ofmorphological traits and firmness, and the RAPD technique was used for the F2 population. Results showed that the recessivepleiotropic gene is responsible for the morphological traits, but environmental and/or genetic factors affect the penetranceand expressivity of the mutation. By the RAPD analysis, a QTL was detected in the group represented by the markers AS-08622,AQ-16747 and l-2 that explains 29.77% of the variation to fruit firmness.

  4. RESISTÊNCIA DE TOMATEIRO (Lycopersicon esculentum Mill. A Stemphylium solani Weber RESISTANCE OF TOMATO (Lycopersicon esculentum Mill. TO THE Stemphylium solani Weber

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    Reinaldo Soares de Paula

    2007-09-01

    ="justify">In the climate conditions of the Brazilian central west regions, tomato can be cultivated the whole year. However, the production is low and quite onerous in function of different factors Besides, different diseases affect the crop. Among the most being the fungal important is the fungi Stemphylium solani, responsible for damages of qualitative and quantitative nature in tomato. With the objective of finding a way to minimize the effect of these pathogen, an experiment was conduced at the experimental fields of the Federal University of Goiás, Goiânia-GO, Brazil. It is located at an altitude of 730 m, latitude of 16° 41’S and longitude of 49° 17’W, with temperature varying among 20 to for 40ºC, relative humidity (average of the month of 80% in August, 83% in September and 85% in October. Precipitation in August, September and October of 1999 was respectively 0.0 mm, 69.6 mm, and 118.4 mm. The main objectives were to test levels of resistance of commercial varieties, genotypes belonging to the bank of germoplasm of Embrapa-CNPH and F1 generations of tomato Lycopersicon esculentum in field conditions to S. solani. The evaluations were accomplished at the 36, 43, 50, 57, 64, 71, 78 and 85 days after the seedlings planting combining diagramatic scale and grades. Was considered the percentage of infected area on the leaf (PIAL and the also grades: 1 – without symptoms; 2 – spraid lesions; 3 – gathering lesions; 4 – partial dryness of the leaf; 5 – death of the leaf and 6 – death of the plant. The differentiation among genotypes was made through analysis of variance of the values of area under disease progress curve and by of Tukey’s test at the level of 5% of probability and also the logistic model. Regarding resistance to S. solani, Ohio 4013, Yoshimatusu and TSW-10 behaved as resistant, among the indeterminate. Among the determined ones, F1 (Hawaii 7998 x Monense showed higher resistance. The genotypes Ohio 4013 and

  5. Phytotoxic effects of a natural bloom extract containing microcystins on Lycopersicon esculentum.

    Science.gov (United States)

    El Khalloufi, Fatima; El Ghazali, Issam; Saqrane, Sana; Oufdou, Khalid; Vasconcelos, Vitor; Oudra, Brahim

    2012-05-01

    The cyanobacterial toxins microcystins (MC) are known to affect many processes in plants. Their presence in the water used for irrigation may have considerable impact on the survivorship, growth and development of plants. In this study, a crude extract of a toxic cyanobacterial bloom from "Lalla Takerkoust" reservoir (Morocco) was used to study the effects of extract containing MC on tomato plants (Lycopersicon esculentum). Five MC variants: MC-LR, MC-FR, MC-LY, MC-(H4)-YR and DMC-LR were identified by HPLC in the cyanobacterial extract. Exposure of the seeds to the crude extract (containing 22.24 μg MC mL(-1)) caused a reduction of germination up to 85%. Experiments showed that 30 days exposure of plant to the cyanobacterial extract containing MC caused inhibition of L. esculentum growth and productivity, as well as harmful effects on photosystem II activity, measured by Fv/Fm fluorescence. An accumulation of nutrients Na(+), K(+) and Ca(2+) was also registered. The activity of peroxidase and phenolic content indicated that the extract caused an oxidative stress. The tissue necrosis of leaves was also a consequence of MC exposure indicating a disorder in the exposed plant metabolism.

  6. INTENSIDAD DE LOS SABORES BÁSICOS DEL TOMATE (Lycopersicon esculentum EN SEIS ESTADOS DE MADUREZ INTENSITY OF BASIC TASTES OF TOMATO (Lycopersicon esculentum IN SIX MATURITY STAGES

    Directory of Open Access Journals (Sweden)

    KATHERIN CASTRO

    2009-06-01

    Full Text Available El tomate (Lycopersicon esculentum, la hortaliza más sembrada en Colombia, es considerada una de las más importantes por su volumen de producción y demanda. La exigencia del consumidor en sus características organolépticas hace necesario un estudio detallado acerca de sus propiedades fisicoquímicas y sensoriales. El análisis sensorial se realizó con un panel entrenado de cinco jueces y las muestras de tomate fueron escogidas de acuerdo con parámetros físicos de color (Cuadro 1 y calidad en los seis estados de madurez del fruto. Se empleó el método de perfil descriptivo, con una escala de respuesta de 0 a 10 puntos, con el fin de cuantificar la intensidad de los sabores básicos (dulce, ácido, amargo, salado y umami. Se realizó un diseño aleatorizado en bloques con seis réplicas por cada tratamiento y un nivel de significancia del 5%. Este indicó la inexistencia de diferencias significativas en las intensidades para cada estado de madurez (p≤ 0,05, y la existencia de diferencias significativas en las intensidades para cada sabor básico evaluado (p>0,05. Mediante el perfil sensorial, se logró establecer que en los estados de madurez 1 y 2, el atributo de mayor intensidad fue el ácido, en los estados de madurez 3, 4 y 5, el atributo que sobresalió fue el dulce y en el estado 6, el atributo percibido en mayor grado fue el umami. Los sabores amargo y salado, se percibieron en una baja intensidad, en los diferentes estados de madurez.The tomato (Lycopersicon esculentum, the vegetable more seeding in the country, is considered one of most important by its volume of production and demand. The exigency of the consumer in sensory characteristics makes necessary a detailed study about its physicochemistry and sensory properties. The sensory analysis was made with a trained panel of five judges and the tomato samples were chosen according to parameters of color and quality in the six ripeness stages of the fruit. Was made using the

  7. Ciclo de maturação e produção de etileno de tomates (Lycopersicon esculentum, Mill. transgênicos Ripening cycle and ethylene production of transgenic tomatoes (Lycopersicon esculentum, Mill.

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    Ana Lúcia CHAVES

    1998-04-01

    Full Text Available Objetivou-se estudar o comportamento fisiológico de frutos de tomateiro (Lycopersicon esculentum, Mill., da cv. Kadá, transformados geneticamente, via Agrobacterium tumefaciens, com o clone de DNA pMEL1, em orientação antisenso, e de frutos desta mesma cultivar, não transformados. O estudo fisiológico foi realizado avaliando-se a duração do ciclo de maturação dos frutos, amadurecidos na planta e após a colheita no estádio verde-maduro, e sua produção de etileno. Os frutos transformados amadurecidos na planta tiveram um ciclo total médio de 27 dias, enquanto os amadurecidos após a colheita, tiveram este intervalo prolongado a 50 dias. Ao contrário, os tomates não transformados apresentaram um ciclo de maturação mais acelerado quando colhidos no estádio verde-maduro, em relação aos frutos amadurecidos nas plantas. Os valores foram, em média, de 20 e 30 dias, respectivamente. Estes resultados estão correlacionados com as variações na produção de etileno observada nos dois genótipos. Frutos não transformados produziram, em média, 10,46 nL de etileno.g-1.h-1, enquanto os transgênicos tiveram sua produção de etileno diminuída para 0,13 nL.g-1.h-1. Pode-se concluir, então, que a redução da produção de etileno, verificada nos tomates transformados, é necessária, mas não é suficiente para prolongar o ciclo de maturação e aumentar a durabilidade dos frutos. Para que isto ocorra, é necessário que se proceda à colheita dos tomates no estádio verde-maduro.This work was carried out to study the physiological behaviour of tomato fruits (Lycopersicon esculentum, Mill., var. Kada, geneticaly transformed, via Agrobacterium tumefaciens, with the pMEL1 DNA clone, in antisense orientation, and nontransformed ones, from the same variety, growth in a greenhouse. The physiological study was conducted for evaluating the ripening cycle of fruits, ripened attached and detached from the plants in the mature-green stage

  8. Origin and evolution of tomato production Lycopersicon esculentum in México

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    Tarsicio Medina Saavedra

    Full Text Available ABSTRACT: Lycopersicon esculentum known as tomato, although has an Andean origin is a contribution of Mexico to the world is, being the first agricultural product to be exported. This research aimed to review the literature in relation to the origin and evolution of the production of tomato in Mexico within the historical development of the country. In ancient times, the tomato was cultivated in milpas (open field and chinampas (artificial islands for riparian agriculture using sustainable methods. Spanish colonizers showed the tomato to the rest of the world and diversified its uses. In independent Mexico, haciendas and railroads integrated the different farming regions. Production decreased during the Mexican revolution, and with land reform, the milpa returned. During the Green Revolution (1970, Sinaloa stood out, with the separation of two systems, subsistence, and modern with technology programs. Biotechnological development (1990 emerged parallel to organic production. So actually with this system, we could return to more sustainable pre-Hispanic ecological principles with less environmental impact.

  9. Effects of hydrogen fluoride on plant-pathogen interactions. [Lycopersicon esculentum; Phaseolus vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    McCune, D.C.; Weinstein, L.H.; Mancini, J.F.; van Lueken, P.

    1973-01-01

    Experiments in fumigation chambers with tomato (Lycopersicon esculentum) and pinto bean (Phaseolus vulgaris) plants were performed to assess the effects of hydrogen fluoride on plant-pathogen (fungal and bacterial) interactions. Hydrogen fluoride was found to alter the plant-pathogen interaction, although the kind and consistency of effect caused by HF depended upon the host, pathogen, and several other factors. A reduction in powdery mildew probably indicates that HF was affecting the infectivity of the pathogen itself because reduction in disease was proportional to the length of the exposure period, infection was continuous during the exposure period, and the pathogen itself was epiphytic. The effect of fluoride on bean rust may have been due to accumulated fluoride in the leaf having a direct or indirect effect on the pathogen because both pre- and post-inoculation exposures to HF were effective and additive. Other evidence for an indirect effect of fluoride was found in halo-blight where stem collapse was affected but foliar symptoms were not, and the site affected was spatially removed from the site of fluoride accumulation. Effects on early blight of tomato also indicated an effect of fluoride in the leaf.

  10. Lycopersicon esculentum Extract Enhances Cognitive Function and Hippocampal Neurogenesis in Aged Mice

    Science.gov (United States)

    Bae, Jung-Soo; Han, Mira; Shin, Hee Soon; Shon, Dong-Hwa; Lee, Soon-Tae; Shin, Chang-Yup; Lee, Yuri; Lee, Dong Hun; Chung, Jin Ho

    2016-01-01

    A decrease in adult neurogenesis is associated with the aging process, and this decrease is closely related to memory impairment. Tomato (Lycopersicon esculentum) is a fruit with diverse bioactive nutrients that is consumed worldwide. In this study, we investigated the cognition-enhancing effect of tomato ethanolic extracts (TEE) in aged mice. Six weeks of oral TEE administration in 12-month-old aged mice significantly increased their exploration time of novel objects when compared to vehicle-treated mice. The TEE supplement increased doublecortin (DCX)-positive cells and postsynaptic density-95 (PSD95) expression in mice hippocampus. Moreover, we found an increased expression of brain-derived neurotrophic factor (BDNF) and subsequently-activated extracellular-signal-regulated kinase (ERK)/cAMP response element binding (CREB) signaling pathway in the TEE-supplemented mice hippocampus. In conclusion, the oral administration of TEE exhibits a cognition-enhancing effect, and the putative underlying mechanism is the induction of BDNF signaling-mediated proliferation and synapse formation in the hippocampus. These findings indicate that TEE could be a candidate for treatment of age-related memory impairment and neurodegenerative disorders. PMID:27792185

  11. EFFECTS OF RADIO FREQUENCY / MICROWAVE ON THE CHEMICAL COMPOSITION AND ANTIOXIDANT POTENTIAL OF LYCOPERSICON ESCULENTUM L.

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    Rammal Marwa

    2013-09-01

    Full Text Available In our work, the effects of radio frequency / microwave on the morphology, chemical composition and the antioxidant power of the plant Lycopersicon esculentum Mill. grown in our laboratory have been accomplished. The obtained results showed that after 10 days of exposure of this plant to electromagnetic fields with high frequency (1250 MHz the stems were long with less leaves than the non exposed plant. However, after 20 days of exposure to this same frequency the stems were long and contain more leaves than the non exposed plant. These leaves were larger and thicker in comparison with those of the non exposed plant. On the other side, the phytochemical screening of the ethanolic extract revealed the presence of flavonoids in the exposed and non exposed plant. Alkaloids, phenols and saponins were only present in non exposed plant. The tannins were absent in the exposed and non exposed plant. Therefore, resins were highly expressed in the exposed plant. On the other side, the x-ray fluorescence indicated the presence of various trace elements more particularly niobium and molybdenum. After exposure, the amount of these elements varies. On the other hand, the DPPH and H2O2 tests showed an important decrease in the antioxidant potential after exposure to studied frequency. This decrease was from 42 % to 18 % at the concentration 0.5 mg/ml. All of these results show that the high frequency emitted by the electromagnetic fields exert a strong effect on the plant and by consequence on human health.

  12. Environmental controls over methanol production, emission, and δ13C values from Lycopersicon esculentum

    Science.gov (United States)

    Oikawa, P.; Giebel, B. M.; Mak, J. E.; Riemer, D. D.; Swart, P. K.; Lerdau, M.

    2009-12-01

    Phytogenic methanol is the dominant source of methanol to the atmosphere, where it is the second most abundant organic compound. Beyond methanol’s role in atmospheric chemistry, it is an indicator of plant function and is linked to plant wound response. Methanol emissions are considered to be a by-product of cell wall expansion and, more specifically, the demethylation of pectin by pectin methylesterase (PME) in cell walls. Production of methanol was investigated in mature and immature tomato Lycopersicon esculentum via measurement of methanol flux, foliar PME activity, and methanol extraction from leaf, root, and stem tissues. δ13C values for mature and immature methanol emissions were also measured using a GC-IRMS system. Environmental control over methanol production and emission was studied by changing temperature and light while holding stomatal conductance constant. As seen previously, mature leaf methanol emissions were significantly less than immature emissions. Surprisingly, preliminary results suggest mature leaf methanol production to be similar to immature leaves, indicating an enhanced metabolic sink for methanol in mature leaves. These data enhance our understanding of methanol production, a term which is not well constrained in current methanol flux models.

  13. Effect of salinity on tomato (Lycopersicon esculentum Mill.) during seed germination stage.

    Science.gov (United States)

    Singh, Jogendra; Sastry, E V Divakar; Singh, Vijayata

    2012-01-01

    A study was conducted using ten genetically diverse genotypes along with their 45F1 (generated by diallel mating) under normal and salt stress conditions. Although, tomato (Lycopersicon esculentum Mill.) is moderately sensitive to salinity but more attention to salinity is yet to be required in the production of tomato. In present study, germination rate, speed of germination, dry weight ratio and Na(+)/K(+) ratio in root and shoot, were the parameters assayed on three salinity levels; control, 1.0 % NaCl and 3.0 % NaCl with Hoagland's solution. Increasing salt stress negatively affected growth and development of tomato. When salt concentration increased, germination of tomato seed was reduced and the time needed to complete germination lengthened, root/shoot dry weight ratio was higher and Na(+) content increased but K(+) content decreased. Among the varieties, Sel-7 followed by Arka Vikas and crosses involving them as a parent were found to be the more tolerant genotypes in the present study on the basis of studied parameters.

  14. Simultaneous ozone fumigation and fluoranthene sprayed as mists negatively affected cherry tomato (Lycopersicon esculentum Mill).

    Science.gov (United States)

    Oguntimehin, Ilemobayo; Eissa, Fawzy; Sakugawa, Hiroshi

    2010-07-01

    Ozone (O(3)) fumigated at 120 microg L(-1) for 12 hd(-1) was combined with 10 microM fluoranthene, and other treatments, including Mannitol solution to investigate the interaction of the two pollutants on tomato plant (Lycopersicon esculentum Mill). Using ten treatments including Mannitol solution and a control, exposure experiment was conducted for 34 d inside six growth chambers used for monitoring the resulted ecophysiological changes. Visible foliar injury, chlorophyll a fluorescence, leaf pigment contents, CO(2) uptake and water vapor exchange were monitored in tomato. Ozone or fluoranthene independently affected some ecophysiological traits of the tomato. In addition, simultaneous treatments with the duo had increased (additive) negative effects on the photosynthesis rate (A(max)), stomatal conductance (g(s)), chlorophyll pigment contents (Chl a, Chl b and Chl((a+b))) and visible foliar symptoms. Contrarily, alleviation of the negative effects of O(3) on the leaf chlorophyll a fluorescence variables by fluoranthene occurred. Mannitol solution, which functioned as a reactive oxygen species scavenger was able to mitigate some negative effects of the two pollutants on the tomato plants.

  15. Uptake, localization, and speciation of cobalt in Triticum aestivum L. (wheat) and Lycopersicon esculentum M. (tomato).

    Science.gov (United States)

    Collins, Richard N; Bakkaus, Estelle; Carrière, Marie; Khodja, Hicham; Proux, Olivier; Morel, Jean-Louis; Gouget, Barbara

    2010-04-15

    The root-to-shoot transfer, localization, and chemical speciation of Co were investigated in a monocotyledon (Triticum aestivum L., wheat) and a dicotyledon (Lycopersicon esculentum M., tomato) plant species grown in nutrient solution at low (5 muM) and high (20 muM) Co(II) concentrations. Cobalt was measured in the roots and shoots by inductively coupled plasma-mass spectrometry. X-ray absorption spectroscopy measurements were used to identify the chemical structure of Co within the plants and Co distribution in the leaves was determined by micro-PIXE (particle induced X-ray emission). Although the root-to-shoot transport was higher for tomato plants exposed to excess Co, both plants appeared as excluders. The oxidation state of Co(II) was not transformed by either plant in the roots or shoots and Co appeared to be present as Co(II) in a complex with carboxylate containing organic acids. Cobalt was also essentially located in the vascular system of both plant species indicating that neither responded to Co toxicity via sequestration in epidermal or trichome tissues as has been observed for other metals in metal hyperaccumulating plants.

  16. Expression of human coagulation Factor IX in transgenic tomato (Lycopersicon esculentum).

    Science.gov (United States)

    Zhang, Hui; Zhao, Lingxia; Chen, Yuhui; Cui, Lijie; Ren, Weiwei; Tang, Kexuan

    2007-10-01

    In the present study, a plant binary expression vector PG-pRD12-hFIX (where PG is polygalacturonase) harbouring the hFIX (human coagulation Factor IX) gene was constructed and introduced into tomato (Lycopersicon esculentum) via Agrobacterium tumefaciens-mediated transformation. After kanamycin selection, 32 putative independent transgenic tomato plants were regenerated. PCR and Southern-blot analyses confirmed the transgenic status of some plants. RT (reverse transcription)-PCR analysis for the expression of the introduced gene (hFIX) demonstrated that the hFIX gene was expressed specifically in fruits of the tomato. Western-blot analysis confirmed the presence of a 56 kDa band specific to hFIX in the transformed tomatoes. ELISA results showed that the expression of hFIX protein reached a maximum of 15.84 ng/g fresh weight in mature fruit. A blood-clotting assay demonstrated the clotting activity of the expressed hFIX protein in transgenic tomato fruits. This is the first report on the expression of hFIX in plants, and our research provides potentially valuable knowledge for further development of the plant-derived therapeutic proteins.

  17. Hygienic quality of traditional processing and stability of tomato (Lycopersicon esculentum) puree in Togo.

    Science.gov (United States)

    Ameyapoh, Yaovi; de Souza, Comlan; Traore, Alfred S

    2008-09-01

    Microbiological and physicochemical qualities of a tomato (Lycopersicon esculentum) puree production line (ripe tomato, washing, cutting, pounding, bleaching, straining, bottling and pasteurization) and its preservation in Togo, West Africa, were studied using the HACCP method. Samples generated during the steps described previously were analyzed by determining sensory, chemical and microbiological characteristics. Samples were analyzed using MPN for coliform populations and plate count methodology for other bacteria. The microorganisms involved in spoilage of the opened products were moulds of genera Penicillium, Aspergillus, Fusarium, Geotrichum, Mucor and gram-positive Bacillus bacteria. The preserved tomato puree exhibited a pH value of 4.3, 90% water content, 0.98 water activity (aw) and an average ascorbic acid level of 27.3mg/100g. Results showed that the critical control point (CCP) of this tomato puree processing line is the pasteurization stage. The analysis of selected microbiological and physicochemical parameters during the preservation of bottled tomato puree indicated that this product was stable over 22 months at 29 degrees C. But the stability of the opened product stored at 29 degrees C did not exceed two months.

  18. Season-controlled changes in biochemical constituents and oxidase enzyme activities in tomato (Lycopersicon esculentum Mill.).

    Science.gov (United States)

    Sen, Supatra; Mukherji, S

    2009-07-01

    Season-controlled changes in biochemical constituents viz. carotenoids (carotene and xanthophyll) and pectic substances along with IAA-oxidase and polyphenol oxidase (PPO) enzyme activities were estimated/assayed in leaves of Lycopersicon esculentum Mill. (tomato) in two developmental stages--pre-flowering (35 days after sowing) and post-flowering (75 days after sowing) in three different seasons--summer rainy and winter Carotenoid content along with pectic substances were highest in winter and declined significantly in summer followed by rainy i.e. winter > summer > rainy. Carotenoid content was significantly higher in the pre-flowering as compared to post-flowering in all three seasons while pectic substances increased in the post-flowering as compared to pre-flowering throughout the annual cycle. IAA oxidase and PPO enzyme activities were enhanced in rainy and decreased sharply in summer and winter i.e. rainy > summer > winter. Both the enzymes exhibited higher activity in the post-flowering stage as compared to pre-flowering in all three seasons. These results indicate winter to be the most favourable season for tomato plants while rainy season environmental conditions prove to be unfavourable (stressful) with diminished content of carotenoid and pectic substances and low activities of IAA oxidase and PPO, ultimately leading to poor growth and productivity.

  19. Lycopersicon esculentum Extract Enhances Cognitive Function and Hippocampal Neurogenesis in Aged Mice

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    Jung-Soo Bae

    2016-10-01

    Full Text Available A decrease in adult neurogenesis is associated with the aging process, and this decrease is closely related to memory impairment. Tomato (Lycopersicon esculentum is a fruit with diverse bioactive nutrients that is consumed worldwide. In this study, we investigated the cognition-enhancing effect of tomato ethanolic extracts (TEE in aged mice. Six weeks of oral TEE administration in 12-month-old aged mice significantly increased their exploration time of novel objects when compared to vehicle-treated mice. The TEE supplement increased doublecortin (DCX-positive cells and postsynaptic density-95 (PSD95 expression in mice hippocampus. Moreover, we found an increased expression of brain-derived neurotrophic factor (BDNF and subsequently-activated extracellular-signal-regulated kinase (ERK/cAMP response element binding (CREB signaling pathway in the TEE-supplemented mice hippocampus. In conclusion, the oral administration of TEE exhibits a cognition-enhancing effect, and the putative underlying mechanism is the induction of BDNF signaling-mediated proliferation and synapse formation in the hippocampus. These findings indicate that TEE could be a candidate for treatment of age-related memory impairment and neurodegenerative disorders.

  20. Ripening Physiology of Fruit from Transgenic Tomato (Lycopersicon esculentum) Plants with Reduced Ethylene Synthesis.

    Science.gov (United States)

    Klee, H. J.

    1993-07-01

    The physiological effects of reduced ethylene synthesis in a transgenic tomato (Lycopersicon esculentum) line expressing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase enzyme have been examined. Fruit from the transgenic line 5673 ripen significantly slower than control fruit when removed from the vine early in ripening. In contrast, fruit that remain attached to the plants ripen much more rapidly, exhibiting little delay relative to the control. Ethylene determinations on attached fruit revealed that there was significantly more internal ethylene in attached than detached fruit. The higher ethylene content can fully account for the observed faster on-the-vine ripening. All of the data are consistent with a catalytic role for ethylene in promoting many, although not all, aspects of fruit ripening. Biochemical analyses of transgenic fruit indicated no significant differences from controls in the levels of ACC oxidase or polygalacturonase. Because transgenic fruit are significantly firmer than controls, this last result indicates that other enzymes may have a significant role in fruit softening.

  1. Características fisiológicas de microtomateiros fitocromo-mutantes Physiological characteristics of micro-tomato (Lycopersicon esculentum P. Miller phytochrome-mutants

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    Hyrandir Cabral de Melo

    2009-10-01

    Full Text Available Objetivou-se, neste trabalho, caracterizar aspectos fisiológicos de microtomateiros (Lycopersicon esculentum P. Miller cv. Micro-Tom fitocromo-mutantes. A cultivar Micro-Tom e os mutantes aurea (deficiente na biossíntese do cromóforo dos fitocromos, atroviolacea (atv e high pigment1 (hp1;ambos superexpressam eventos mediados por fitocromos foram cultivados em condições controladas de luz e temperatura e caracterizados no estágio de floração. O mutante hp1 obteve as maiores taxas de fotossíntese potencial e de conteúdo de carotenóides. O mutante aurea manteve taxas de fotossíntese potencial similares à cultivar Micro-Tom, mesmo expressando o mais baixo conteúdo de clorofilas, e também expressou o maior conteúdo de nitrogênio entre os demais microtomateiros. Os mutantes aurea e hp1 obtiveram os menores conteúdos de açúcares solúveis totais. O mutante atv expressou o maior conteúdo de clorofilas e também a menor razão clorofila a/b.The objective of this work was to characterize physiological aspects of micro-tomato (Lycopersicon esculentum P. Miller cv. Micro-Tom phytochrome-mutants. Plants of Micro-Tom cultivar and aurea (deficient in phytochrome chromophore biosynthesis, high pigment1 (hp1 and atroviolacea (atv (both super express phytochrome events-mediated mutants were cultivated under controlled light and temperature and evaluated in flowering stage. The hp1 mutant expressed the highest rates of potential photosynthesis and also the content of total carotenoids. Aurea mutant maintained similar potential photosynthesis rates as the Micro-Tom cultivar, even containing low chlorophyll content, and expressed the highest content of nitrogen among all micro-tomatoes studied. Total soluble sugars were lower in aurea and hp1 mutants. The atv mutant expressed the highest content of chlorophylls and also the lowest rate of chlorophyll a/b.

  2. EVALUACIÓN DE CALIDAD EN FRUTOS DE SIETE GENOTIPOS NATIVOS DE JITOMATE (Lycopersicon esculentum var. cerasiforme)

    OpenAIRE

    2009-01-01

    El objetivo de este estudio fue evaluar la calidad en frutos de siete genotipos nativos de jitomate (Lycopersicon esculentum var. cerasiforme): JCPRV-05, JCPVR-09, JCPRV-10, JCPRV-43, JCPRV-70, JCPRV-71, y JCPRV-76, cultivados en hidroponía sin recirculación y bajo invernadero. Como testigo se usó un híbrido comercial de jitomate tipo 'cherry' (H-790). Se evaluó firmeza, días para alcanzar 7 % de pérdida de peso (DPP), pH, sólidos solubles totales, acidez titulable, contenido de licopeno, de ...

  3. Antioxidant Activity of Tomato (Lycopersicon esculentum L.) of Low Soluble Solids and Development of a Shelf Stable Spread

    OpenAIRE

    Balaswamy K; Prabhakara Rao PG; Pragati Yadav; Narsing Rao G; Sulochanamma G; Satyanarayana A

    2015-01-01

    Tomato (Lycopersicon esculentum L.) is known to be associated with a reduced risk of developing a certain chronic diseases and cancers due to the presence of lycopene, a bioactive carotenoid. Commercially, the vegetable is used in the preparation of tomato puree or ketchup, but the local varieties with lower total soluble solids was found to be not useful for the same and fetching not enough prices during glut and being wasted without processing. In the present study, the local...

  4. Enhanced biosynthesis of quercetin occurs as A photoprotective measure in Lycopersicon esculentum mill. under Acute UV-B exposure

    Directory of Open Access Journals (Sweden)

    Abhilasha Shourie

    2014-06-01

    Full Text Available Lycopersicon esculentum respond to UV-B by enhanced synthesis of flavonoid quercetin, a strong antioxidant that helps the plants to well acclimatize to UV-B stress. Three weeks old plants of L. esculentum were subjected to acute UV-B irradiation for 20, 40 and 60 minutes daily until 28 days and analyzed for the morphological and biochemical changes. UV-B exposure for 40 and 60 minutes considerably affected the growth and biomass of L. esculentum. The leaves were deformed, developed chlorosis and abscised early as compared to the unexposed plants. Biomass declined by 35% and total chlorophyll decreased by 24.7% due to disintegration of chloroplasts. Enhancement was seen in the content of carotenoids, anthocyanins and total flavonoids by 15, 33.3 and 22.8%, respectively, which was attributed to the photoprotective role of these compounds as potential quenchers of excess excitation energy. Quercetin content decreased on UV-B exposure to 20 and 40 min, and thereafter increased significantly by 5.19% on 60 min of exposure. This pattern probably indicated that the over-expression of genes involved in its biosynthesis such as phenylalanine ammonia lyase (PAL, chalcone synthase (CHS, flavanone 3-hydroxylase (F3H and dihydroflavonol 4-reductase (DFR occurred only after certain threshold exposure (60 min, which could be the strategy for developing tolerance against UV-B stress in L. esculentum.

  5. In vitro and in vivo inoculation of four endophytic bacteria on Lycopersicon esculentum.

    Science.gov (United States)

    Botta, Anna Lucia; Santacecilia, Alessandra; Ercole, Claudia; Cacchio, Paola; Del Gallo, Maddalena

    2013-09-25

    Four bacteria selected on the basis of their capability of fixing atmospheric nitrogen, stimulating plant-growth, and protecting the host plant from pathogens - Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, Burkholderia ambifaria - were inoculated on tomato seeds either singularly, in couple and in a four bacteria mixer. Aim of this research was to evaluate: (1) effect of single and mixed cultures on the inoculated plant - plant growth, dry weight, root length and surface, number of leaves, among others; (2) colonization and interactions of the bacteria inside the host plant; (3) localization inside the host of single bacterial strains marked with the gusA reporter gene. The results obtained indicate that all selected microbial strains have colonized Lycopersicon esculentum but in a different way, depending on the single species. A. brasilense, G. diazotrophicus inoculated in vitro singularly and together were the best plant colonizers. In vivo essays, instead, B. ambifaria and the four-bacteria mixer gave the best results. It was possible to localize both A. brasilense and H. seropedicae inside the plant by the gusA reporter gene. The bacterial strains occur along the root axis from the apical zone until to the basal stem, on the shoot from the base up to the leaves. The four bacteria actively colonize tomato seeds and establish an endophytic community inside the plant. This review gives new information about colonization processes, in particular how bacteria interact with plants and whether they are likely to establish themselves in the plant environment after field application as biofertilizers or biocontrol agents.

  6. Retention of Photoinduction of Cytosolic Enzymes in aurea Mutant of Tomato (Lycopersicon esculentum).

    Science.gov (United States)

    Goud, K. V.; Sharma, R.

    1994-06-01

    The tomato (Lycopersicon esculentum Mill.) aurea (au) mutant has been characterized as a phytochrome-deficient mutant lacking spectrally detectable phytochrome A in etiolated seedlings. Seedlings of au grown under red light (RL) lack phytochrome regulation of nuclear genes encoding plastidic proteins, possess ill-developed chloroplasts, and are slow to de-etiolate. In the present study, the effect of phytochrome deficiency on photoinduction of enzymes in etiolated au seedlings was investigated. The photoinduction of the cytosolic enzymes amylase and nitrate reductase (NR) and of the plastidic enzyme nitrite reductase (NiR) in au was compared with that in the isogenic wild-type (WT) tomato and the high-pigment (hp) mutant with exaggerated phytochrome response. In WT and hp, both brief RL pulses and continuous RL induced amylase, NR, and NiR activities, whereas in au no photoinduction of enzymes was observed with brief RL pulses, and continuous RL induced only amylase and NR activities. The time courses of photoinduction of NR and amylase in au under continuous RL followed patterns qualitatively similar to hp and WT. A blue-light pretreatment prior to continuous RL exposure was ineffective in inducing NiR activity in au. Only continuous white light could elicit a photoinduction of NiR in au seedlings. The norflurazon-triggered loss of photoinduction of NiR in WT and hp indicated that NiR photoinduction depended on chloroplast biogenesis. The results indicate that observed photoinduction of NR and amylase in au may be mediated by a residual phytochrome pool.

  7. Production and characterisation of monoclonal antibodies to phytoene synthase of Lycopersicon esculentum.

    Science.gov (United States)

    Fraser, P D; Misawa, N; Sandmann, G; Johnson, J; Schuch, W; Bramley, P M

    1998-10-01

    Monoclonal antibodies have been prepared against the tomato (Lycopersicon esculentum Mill.) fruit ripening-enhanced phytoene synthase (PSY1). The antigen was prepared as a beta-galactosidase fusion protein by cloning a 1.13 kb fragment of Psy1 cDNA into pUR291, followed by transformation of E. coli. The fusion protein, induced by IPTG, was purified by preparative SDS-PAGE and used to elicit an immune response. The cell lines were screened for cross-reactivity against beta-galactosidase-phytoene synthase fusion protein in E. coli extracts using western blotting and ELISA detection procedures. Positive clones were further screened for their ability to cross-react with the mature phytoene synthase protein on western blots as well as their ability to inhibit enzyme activity. Eleven monoclonal lines were obtained. Nine of these, all of the IgM isotype, exhibited strong responses to phytoene synthase of ripe tomato fruit on western blots, but did not inhibit enzyme activity effectively. The other two lines (IgG/la 2 isotypes) inhibited phytoene synthase activity in ripe tomato stroma, but produced a poor response to the protein on western blots. The monoclonals identified a ripe fruit phytoene synthase of 38 kDa, exclusively located in the chromoplast. In contrast, antibodies were unable to detect microbial phytoene synthases, nor phytoene synthase of maize leaf, tomato chloroplast or mango fruit extracts, either on western blots or from inhibition of phytoene synthase activity. However, they did cross-react with a 44 kDa protein from carrot leaf stroma and with three different proteins (44, 41, and 37 kDa) in carrot root. Cross-reactivity was also found with a 37 kDa protein from pumpkin fruit stroma.

  8. Cloning of tomato (Lycopersicon esculentum Mill.) arginine decarboxylase gene and its expression during fruit ripening.

    Science.gov (United States)

    Rastogi, R; Dulson, J; Rothstein, S J

    1993-11-01

    Arginine decarboxylase (ADC) is the first enzyme in one of the two pathways of putrescine biosynthesis in plants. The genes encoding ADC have previously been cloned from oat and Escherichia coli. Degenerate oligonucleotides corresponding to two conserved regions of ADC were used as primers in polymerase chain reaction amplification of tomato (Lycopersicon esculentum Mill.) genomic DNA, and a 1.05-kb fragment was obtained. This genomic DNA fragment encodes an open reading frame of 350 amino acids showing about 50% identity with the oat ADC protein. Using this fragment as a probe, we isolated several partial ADC cDNA clones from a tomato pericarp cDNA library. The 5' end of the coding region was subsequently obtained from a genomic clone containing the entire ADC gene. The tomato ADC gene contains an open reading frame encoding a polypeptide of 502 amino acids and a predicted molecular mass of about 55 kD. The predicted amino acid sequence exhibits 47 and 38% identify with oat and E. coli ADCs, respectively. Gel blot hybridization experiments show that, in tomato, ADC is encoded by a single gene and is expressed as a transcript of approximately 2.2 kb in the fruit pericarp and leaf tissues. During fruit ripening the amount of ADC transcript appeared to peak at the breaker stage. No significant differences were seen when steady-state ADC mRNA levels were compared between normal versus long-keeping Alcobaca (alc) fruit, although alc fruit contain elevated putrescine levels and ADC activity at the ripe stage. The lack of correlation between ADC activity and steady-state mRNA levels in alc fruit suggests a translational and/or posttranslational regulation of ADC gene expression during tomato fruit ripening.

  9. Phytochemical and nutrient/antinutrient interactions in cherry tomato (Lycopersicon esculentum) fruits.

    Science.gov (United States)

    Oyetayo, Folake Lucy; Ibitoye, Muyiwa Femi

    2012-07-01

    The fruit of the cherry tomato (Lycopersicon esculentum (Solanaceae)) was analysed for mineral and antinutrient composition. Phosphorus (33.04 ± 0.21 mg/100g) was the most abundant mineral in the fruit, followed by calcium (32.04 ± 0.06 mg/100 g), and potassium (11.9 ± 0.1 mg/100 g) and manganese (9.55 ± 0.28 mg/100 g) were also present in appreciable quantities. Antinutrients, including phytate, glycoside, saponin and tannin, were screened and quantified. Phytate (112.82 ± 0.1 mg/100 g), glycoside (2.33 ± 0.00 mg/100 g), saponin (1.31 ± 0.00 mg/100g) and tannin (0.21 ± 0.00 mg/100 g) were present in the fruit but phlobatanin and glycosides with steroidal rings were not found. The calculated calcium:phytate ratio of the fruits was below the critical value and the calculated [calcium] [phytate]:[zinc] molar ratio was less than the critical value. The calcium:phosphorus ratio (0.97 mg/100 g) shows the fruit to be a good source of food nutrients, while the sodium:potassium value was less than 1. Ca/P ratio below 0.5 indicates deficiency of these minerals while Na/K ratio above 1 is detrimental because of excessive sodium levels. The results of the study generally revealed the fruit to be rich in minerals but containing insufficient quantities of antinutrients to result in poor mineral bioavailability.

  10. Padrão de identidade e qualidade do tomate (Lycopersicon esculentum Mill. de mesa

    Directory of Open Access Journals (Sweden)

    Ferreira Sila Mary Rodrigues

    2004-01-01

    Full Text Available O valor comercial do tomate, Lycopersicon esculentum Mill., é definido pelas características de identidade e qualidade presentes no fruto que podem ser avaliadas pela classificação do produto. A presente revisão de literatura teve como objetivo descrever o padrão de identidade e qualidade do tomate de mesa, através da classificação. O tomate de mesa é classificado em grupos, subgrupos, classes e tipos. De acordo com o formato do fruto, o tomate é classificado em dois grupos: oblongo, quando o diâmetro longitudinal é maior que o transversal e redondo, quando o diâmetro longitudinal é menor ou igual ao transversal. Em função do estádio de maturação, o tomate é classificado em cinco subgrupos: verde maduro, pintado, rosado, vermelho e vermelho maduro. A classe é definida em função do maior diâmetro transversal do fruto em relação ao grupo a que pertença. O tomate oblongo é classificado em três classes: grande, médio e pequeno. O tomate redondo é classificado em quatro classes: gigante, grande, médio e pequeno. De acordo com os índices de ocorrência de defeitos graves e leves presentes nos frutos, o tomate é classificado também em tipo: extra, especial ou selecionado e comercial. São considerados defeitos graves: podridão, passado, queimado, dano por geada, podridão apical, e defeitos leves: dano, mancha, ocado, deformado e imaturo. A amostra de tomate que ultrapasse os índices de defeitos previstos pela legislação do Ministério da Agricultura é classificada como fora do padrão.

  11. Genealogy and fine mapping of obscuravenosa, a gene affecting the distribution of chloroplasts in leaf veins, and evidence of selection during breeding of tomatoes (Lycopersicon esculentum; Solanaceae).

    Science.gov (United States)

    Jones, Carl M; Rick, Charles M; Adams, Dawn; Jernstedt, Judy; Chetelat, Roger T

    2007-06-01

    In the processes of plant domestication and variety development, some traits are under direct selection, while others may be introduced by indirect selection or linkage. In the cultivated tomato (Lycopersicon esculentum = Solanum lycopersicum), and all other Solanaceae examined, chloroplasts are normally absent from subepidermal and mesophyll cells surrounding the leaf veins, and thus, veins appear clear upon subillumination. The tomato mutant obscuravenosa (obv), in contrast, contains chloroplasts in cells around the vein, and thus, veins appear as dark as the surrounding leaf tissue. Among tomato cultivars, the obv allele is common in processing varieties bred for mechanical harvest, but is otherwise rare. We traced the source of obv in processing tomatoes to the cultivar Earliana, released in the 1920s. The obv locus was mapped to chromosome 5, bin 5G, using introgression lines containing single chromosome segments from the wild species L. pennellii. This region also contains a quantitative trait locus (QTL) for plant height, pht5.4, which cosegregated with SP5G, a paralog of self-pruning (sp), the gene that controls the switch between determinate and indeterminate growth in tomato. The pht5.4 QTL was partially dominant and associated with a reduced percentage of red fruit at harvest. Our data suggest that the prevalence of obv in nearly all processing varieties may have resulted from its tight linkage to a QTL conferring a more compact, and horticulturally desirable, plant habit.

  12. Low-temperature-related growth and photosynthetic performance of alloplasmic tomato (Lycopersicon esculentum Mill.) with chloroplasts from L-hirsutum Humb. & Bonpl.

    NARCIS (Netherlands)

    Dolstra, O; Venema, JH; Groot, PJ; van Hasselt, PR

    2002-01-01

    Growth and photosynthetic performance were analyzed in alloplasmic tomato at a high- (25/17degreesC; HTR) and low-temperature regime (12/6degreesC; LTR) in order to establish the role of cytoplasmic variation on low-temperature tolerance of tomato ( Lycopersicon esculentum Mill.). Four alloplasmic t

  13. Salt-mediated changes in leaf mesophyll cells of Lycopersicon esculentum Mill. plants

    Directory of Open Access Journals (Sweden)

    Magdalena Gapinska

    2014-09-01

    Full Text Available Five-week-old tomato plants (Lycopersicon esculentum cv. Perkoz grown in pots containing garden soil in a growth chamber were submitted to 50 or 150 mM NaCl for 1 h, 2 and 5 days. Tomato leaf anatomy generally did not change after short time salinity, except 5-day-treatment with 150 mM NaCl, where changed cell shape (shrunk and deformed simultaneously with increased volume of intercellular spaces (IS were observed. Although leaf hydration (H depleted only 1 h after 150 mM NaCl treatment both salt concentrations generated two coexisting populations of salt-affected mesophyll cells: (i slightly-affected (Sl-A which showed incipient plasmolysis or slightly changed shapes, and (ii severely-affected (Sv-A which showed severe plasmolysis; serious deformation of cell shape or disorganization including cell degeneration. In Sl-A cells salinity changed location and shape of chloroplasts which were: more rounded, with oversized starch grains (SG (2d or more flat (5d. Salt-mediated changes were becoming more distinguished and pronounced with length of 150 mM NaCl treatment. The amount of salt-affected cells was changing during the experiment and depended on the salt concentration. In 50 mM-treated plants salt-affected cells appeared 1 h after treatment (~40% and raised up to 78% on 2nd day, however the population of Sl-A cells dominated. In 150 mM NaCl-treated plants the percentage of affected cells raised during the experiment from 75% to 99%. Firstly Sl-A cells dominated, but on the 5th day the majority was Sv-A. Salt-affected cells were distributed quite evenly in palisade or spongy mesophyll, except 2 d after treatment with 50 mM NaCl, when their number was higher in the palisade mesophyll. Sv-A cells in the spongy mesophyll were located mostly near the bundle while in the palisade mesophyll more irregularly. Different susceptibility of cells to salt stress might be the consequence of an unequal distribution of osmotic stress and subsequent ionic

  14. Relationships between fruit exocarp antioxidants in the tomato (Lycopersicon esculentum) high pigment-1 mutant during development.

    Science.gov (United States)

    Andrews, Preston K.; Fahy, Deirdre A.; Foyer, Christine H.

    2004-04-01

    Development-dependent changes in fruit antioxidants were examined in the exocarp (epidermal and hypodermal tissues) of the monogenic recessive tomato (Lycopersicon esculentum L.) mutant high pigment (hp-1) and its wild-type parent 'Rutgers' grown under non-stress conditions in a greenhouse. The hp-1 mutant was chosen for this study because the reportedly higher lycopene and ascorbic acid (AsA) contents of the fruit may alter its tolerance to photooxidative stress. Throughout most of fruit development, reduced AsA concentrations in the exocarp of hp-1 were 1.5 to 2.0 times higher than in 'Rutgers', but total glutathione concentrations were similar in both genotypes. Only in ripe red fruit were reduced AsA and total glutathione concentrations lower in hp-1 than in 'Rutgers'. The redox ratios (reduced : reduced + oxidized) of AsA in hp-1 and 'Rutgers' exocarps were similar and usually > 0.9, however, the redox ratio of glutathione was lower in hp-1 than in 'Rutgers' throughout development. Lycopene concentrations in ripe red fruit were about 5 times higher in hp-1 than in 'Rutgers'. Large increases in the specific enzyme activities of superoxide dismutase (EC 1.15.1.1), ascorbate peroxidase (EC 1.11.1.11), and monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) occurred during fruit development in both genotypes, with an inverse relationship between the activities of these enzymes and chlorophyll content. Glutathione reductase (EC 1.6.4.2) and MDHAR-specific activities were higher in hp-1 than 'Rutgers' only at the later stages of fruit development. Dehydroascorbate reductase (EC 1.8.5.1) activities, however, were usually higher in 'Rugters' than in hp-1. Catalase (CAT, EC 1.11.1.6) activities increased with fruit development until the fruit were orange/light red, when CAT was higher in 'Rutgers' than in hp-1, but then declined in the ripe red fruit of both genotypes. These results suggest that elevated AsA in the exocarp of hp-1 fruit early in fruit development may

  15. A molecular linkage map of tomato displaying chromosomal locations of resistance gene analogs based on a Lycopersicon esculentum x Lycopersicon hirsutum cross.

    Science.gov (United States)

    Zhang, L P; Khan, A; Niño-Liu, D; Foolad, M R

    2002-02-01

    A molecular linkage map of tomato was constructed based on a BC1 population (N = 145) of a cross between Lycopersicon esculentum Mill. line NC84173 (maternal and recurrent parent) and Lycopersicon hirsutum Humb. and Bonpl. accession PI126445. NC84173 is an advanced breeding line that is resistant to several tomato diseases, not including early blight (EB) and late blight (LB). PI126445 is a self-incompatible accession that is resistant to many tomato diseases, including EB and LB. The map included 142 restriction fragment length polymorphism (RFLP) markers and 29 resistance gene analogs (RGAs). RGA loci were identified by PCR amplification of genomic DNA from the BC1 population, using ten pairs of degenerate oligonucleotide primers designed based on conserved leucine-rich repeat (LRR), nucleotide binding site (NBS), and serine (threonine) protein kinase (PtoKin) domains of known resistance genes (R genes). The PCR-amplified DNAs were separated by denaturing polyacrylamide gel electrophoresis (PAGE), which allowed separation of heterogeneous products and identification and mapping of individual RGA loci. The map spanned 1469 cM of the 12 tomato chromosomes with an average marker distance of 8.6 cM. The RGA loci were mapped to 9 of the 12 tomato chromosomes. Locations of some RGAs coincided with locations of several known tomato R genes or quantitative resistance loci (QRLs), including Cf-1, Cf-4, Cf-9, Cf-ECP2, rx-1, and Cm1.1 (chromosome 1); Tm-1 (chromosome 2); Asc (chrromosme 3); Pto, Fen, and Prf (chromosome 5); 01-1, Mi, Ty-1, Cm6.1, Cf-2, CF-5, Bw-5, and Bw-1 (chromosome 6); I-1, 1-3, and Ph-1 (chromosome 7); Tm-2a and Fr1 (chromosome 9); and Lv (chromosome 12). These co-localizations indicate that the RGA loci were either linked to or part of the known R genes. Furthermore, similar to that for many R gene families, several RGA loci were found in clusters, suggesting their potential evolutionary relationship with R genes. Comparisons of the present map with

  16. Induction of Volatile Organic Compounds of Lycopersicon esculentum Mill. and Its Resistance to Botrytis cinerea Pers. by Burdock Oligosaccharide

    Institute of Scientific and Technical Information of China (English)

    Pei-Qing He; Li Tian; Kao-Shan Chen; Lin-Hua Hao; Guang-You Li

    2006-01-01

    In the present study, we investigated the induction of volatile organic compounds (VOCs) of Lycopersicon esculentum Mill. and its resistance to Botrytis cinerea Pers. by burdock oligosaccharide. The disease severity of L. esculentum was evaluated 48 h after treatment with 0.6% burdock oligosaccharide, followed by inoculation with a spore suspension of B. cinerea. The formation of O()2, the activity of lipoxygenases (LOX), peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), and the quantity and quality of changes in VOCs were determined a period of time after treatment with 0.6% burdock oligosaccharide. The results demonstrated that the disease index in treated plants was decreased by 42.5% compared with control 96 h after inoculation. The production of O()2 reached a maximum 6 h after treatment (1.36-fold compared with control). There was an increase in LOX, POD, CAT and SOD activity in response to burdock oligosaccharide treatment and the enzymes showed different trends in the time-course of induction. At 120 h after treatment,(E)-2-hexenal was increased by 92% compared with control, whereas methyl salicylate showed a gradual increase with induction period. Previous results had demonstrated that chitosan elicitor enhanced the production VOCs of L. esculentum and decreased plant susceptibility towards B. cinerea. Together, these findings suggest that increasing the production of VOCs in response to burrdock oligosaccharide may be an important mechanism for L. esculentumin its defense against pathogens. In addition, burrdock oligosaccharide may act as a potent elicitor of resistance to disease in L. esculentum.

  17. Capítulo V: evaluación de especies silvestres de Lycopersicon sp. como fuente de resistencia al insecto plaga Scrobipalpula absoluta (Meyrick y su intento de transferencia a Lycopersicon esculentum Mill

    Directory of Open Access Journals (Sweden)

    Vallejo Cabrera Franco Alirio

    1994-12-01

    Full Text Available

    En condiciones de campo, se evaluaron 32 introducciones silvestres de Lycopersicon sp. y una de la especie cultivada, L. esculentum Mill, con el fin de determinar la resistencia a Scrobipalpula absoluta (Meyrick. Se utilizó el diseño experimental bloques completos al azar con 4 repeticiones. Como bordes se colocaron tres variedades comerciales susceptibles al insecto (Chonto "Mata Verde", Napoli Y Chonto "Santa Cruz Kada" con el fin de tener una fuente de infestación natural y permanente del cogollero dentro del experimento. Se evaluaron los caracteres número de cogollos afectados, número de hojas dañadas, número de ampollas e intensidad de daño. Todas las introducciones de L. hirsutum y L. peruvianum fueron altamente resistentes. Las introducciones 1406 y 1407 de L. pimpinellifolium también fueron altamente resistentes. La introducción 1759 de L. esculentum y L. esculentum var. ceraciforme fueron muy susceptibles. Las introducciones de L. pimpinellifolium y L. hirsutum no tuvieron dificultad para hibridarse con L. esculentum var. Tropic. El cruzamiento L. esculentum x L. peruvianum no presentó semilla híbrida y se requirió efectuar cultivo de embriones inmaduros, in vitro, utilizando el medio Murashige & Skoog pero sin lograrse resultados positivos.

    Thirty two (32 wild accessions of Lycopersicon sp. and one (1 cultivated accession of Lycopersicon esculentum Mill were evaluated to determinate the resistance to Scrobipalpula absoluta (Meyrick. The experiment was conducted with a complete random design and four repetitions. Three (3 susceptibles cultivated accessions of L. esculentum (Chonto "Mata Verde", Napoli and Chonto "Santa Cruz Kada" were sowed to have natural infestation of S. absoluta. Damage intensity, number of buds damaged, number of leaves demaged and number of "blister" in the leaves were evaluated, All evaluated accessions of L. peruvianum showed heigh resistence. 1406 and 1407 accessions of L. pimpinellifolium

  18. Nicotine promotes rooting in leaf explants of in vitro raised seedlings of tomato, Lycopersicon esculentum Miller var. Pusa Ruby.

    Science.gov (United States)

    Bamel, Kiran; Gupta, Rajendra; Gupta, Shrish C

    2015-11-01

    Nicotine promotes rooting in leaf explants of tomato (Lycopersicon esculentum Miller var. Pusa Ruby). Nicotine at 10(-9) to 10(-3) M concentrations was added to the MS basal medium. The optimum response (three-fold increase in rooting) was obtained at 10(-7) M nicotine-enriched MS medium. At the same level i.e. 10(-7) M Nicotine induced dramatic increase (11-fold) in the number of secondary roots per root. We have shown earlier that exogenous acetylcholine induces a similar response in tomato leaves. Since nicotine is an agonist of one of the two acetylcholine receptors in animals, its ability to simulate ACh action in a plant system suggests the presence of the same molecular mechanism operative in both, animal and plant cells. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. Phytochrome-mediated induction of phenylalanine ammonia-lyase in the cotyledons of tomato (Lycopersicon esculentum Mill.) plants.

    Science.gov (United States)

    Lercari, B; Sodi, F; Fastami, C

    1982-01-01

    Phenylalanine ammonia-lyase (PAL; EC 4.3.1.5.) induction in cotyledons from 96-h dark-grown Lycopersicon esculentum Mill. was studied in response to continuous light and hourly light pulses (blue, red, far red). The increases of PAL promoted by blue and red pulses are reversed completely by immediately following 758 nm irradiations. The response to continuous red light could be substituted for by hourly 6-min red light pulses. The effect of continuous red treatments is mainly due to a multiple induction effect of phytochrome. In contrast to red light, hourly light pulses with far red and blue, light can only partially substitute for continuous irradiation. The continuous blue response could be due to a combination of a multiple induction response and of a high irradiance response of phytochrome. The continuous far red response, could represent a high irradiance response of phytochrome. Dichromatic irradiations indicate that phytochrome is the photoreceptor controlling the light response (PAL) in tomato seedlings.

  20. Influence of manganese on yielding of tomato (Lycopersicon esculentum Mill. cultivated in rockwool

    Directory of Open Access Journals (Sweden)

    Tomasz Kleiber

    2014-06-01

    Full Text Available The aim of the conducted studies was to determine the effect of different concentrations of manganese in nutrient solution on yielding of tomato (Lycopersicon esculentum Mill. cvs. ‘Alboney F1’ and ‘Emotion F1’ for optimization of the nutrition (experiment I and to determine the threshold of tolerance of plants on the mentioned nutrient (experiment II. Plants were grown in rockwool using a nutrient solution with the differentiated contents of manganese (mg·dm-3: experiment I, 2008-2011 – control (0.06, 0.3, 0.6, 1.2 (described as Mn-0, Mn-0.3, Mn-0.6, Mn-1.2, experiment II, 2012 – 2.4, 4.8, 9.6, 19.2 (described as Mn-2.4, Mn-4.8, Mn-9.6, Mn-19.2. For plant fertigation was used standard nutrient solution with the following chemical composition (mg·dm-3: N-NH4 – 2.2, N-NO3 – 230, P – 50, K – 430, Ca – 145, Mg – 65, Cl – 35, S-SO4 – 120, Fe – 2.48, Zn – 0.50, Cu – 0.07. pH value was 5.50, and EC – 3.00 mS·cm-1. The earliest symptoms of manganese poisoning plants were observed in case of Mn-19.2 – a month and a half after the beginning of the cultivation, and in the case of Mn-9.6 – after 2.5 months of exposure to the nutrient solution. Initial signs of plants poisoning applying manganese Mn-4.8 were observed after a 3-month use of the nutrient solution. To the level of Mn-1.2 there was a significant effect of manganese on the tomatoes yield of class I, III, IV and VI. The variety affected the average yield of class I and V, only. The highest marketable fruit yield of ‘Alboney F1’ was obtained using nutrient solutions with manganese content of 0.3 and 0.6 mg·dm-3. In the case of ‘Emotion F1’ the yield by using Mn-0 and Mn-0.3 was significantly lower than in the case of Mn-0.6. In the application of the nutrient solution containing 1.2 mg Mn per 1 dm3 significant reducing of yielding was found while the plants were not observed for symptoms resulting from excessive manganese nutrition. In range of

  1. Proteínas antifúngicas contra p. infestans en los espacios intercelulares de hojas de tomate (lycopersicon esculentum cerasiforme) posibles defensinas de plantas

    OpenAIRE

    Luz Dary Riaño; Humberto Miguel Zamora

    2008-01-01

    Se aislaron y caracterizaron parcialmente proteínas antifúngicas de los espacios intercelulares de hojas de tomate Lycopersicon esculentum cerasiforme, variedad que ha mostrado resistencia en campo a Phytophthora Infestans; se observó quedespués de inoculación con el patógeno dichas proteínas se acumularon sistémicamente en la planta. Las proteínas identificadasmostraron características de Defensinas de plantas, una nueva f...

  2. Polyamine metabolism in ripening tomato fruit. I. Identification of metabolites of putrescine and spermidine. [Lycopersicon esculentum Mill

    Energy Technology Data Exchange (ETDEWEB)

    Rastogi, R.; Davies, P.J. (Cornell Univ., Ithaca, NY (USA))

    1990-11-01

    The metabolism of (1,4-{sup 14}C)putrescine and (terminal methylene-{sup 3}H)spermidine was studied in the fruit pericarp (breaker stage) discs of tomato (Lycopersicon esculentum Mill.) cv Rutgers, and the metabolites identified by high performance liquid chromatography and gas chromatography-mass spectrometry. The metabolism of both putrescine and spermidine was relatively slow; in 24 hours about 15% of each amine was metabolized. The {sup 14}C label from putrescine was incorporated into spermidine, {gamma}-aminobutyric acid (GABA), glutamic acid, and a polar fraction eluting with sugars and organic acids. In the presence of gabaculine, a specific inhibitor of GABA:pyruvate transminase, the label going into glutamic acid, sugars and organic acids decreased by 80% while that in GABA increased about twofold, indicating that the transamination reaction is probably a major fate of GABA produced from putrescine in vivo. ({sup 3}H)Spermidine was catabolized into putrescine and {beta}-alanine. The conversion of putrescine into GABA, and that of spermidine into putrescine, suggests the presence of polyamine oxidizing enzymes in tomato pericarp tissues. The possible pathways of putrescine and spermidine metabolism are discussed.

  3. Products Released from Enzymically Active Cell Wall Stimulate Ethylene Production and Ripening in Preclimacteric Tomato (Lycopersicon esculentum Mill.) Fruit.

    Science.gov (United States)

    Brecht, J K; Huber, D J

    1988-12-01

    Enzymically active cell wall from ripe tomato (Lycopersicon esculentum Mill.) fruit pericarp release uronic acids through the action of wall-bound polygalacturonase. The potential involvement of products of wall hydrolysis in the induction of ethylene synthesis during tomato ripening was investigated by vacuum infiltrating preclimacteric (green) fruit with solutions containing pectin fragments enzymically released from cell wall from ripe fruit. Ripening initiation was accelerated in pectin-infiltrated fruit compared to control (buffer-infiltrated) fruit as measured by initiation of climacteric CO(2) and ethylene production and appearance of red color. The response to infiltration was maximum at a concentration of 25 micrograms pectin per fruit; higher concentrations (up to 125 micrograms per fruit) had no additional effect. When products released from isolated cell wall from ripe pericarp were separated on Bio-Gel P-2 and specific size classes infiltrated into preclimacteric fruit, ripening-promotive activity was found only in the larger (degree of polymerization >8) fragments. Products released from pectin derived from preclimacteric pericarp upon treatment with polygalacturonase from ripe pericarp did not stimulate ripening when infiltrated into preclimacteric fruit.

  4. Olive mill wastewater triggered changes in physiology and nutritional quality of tomato (Lycopersicon esculentum mill) depending on growth substrate.

    Science.gov (United States)

    Ouzounidou, G; Asfi, M; Sotirakis, N; Papadopoulou, P; Gaitis, F

    2008-10-30

    We have studied the changes in the physiology and nutritional quality of Lycopersicon esculentum exposed to olive mill wastewater (OMW) with regard to cultivation in sand and soil. Tomato plant performance decreased with increasing concentration of OMW to both substrates. Root was more sensitive to OMW than the upper parts of the plants, grown either in sand or in soil for 10 days and 3 months, respectively, probably due to the direct OMW toxicity on roots as compared to other parts. Significant restriction on uptake and translocation of nutrients (K, Na, Fe, Ca and Mg) under OMW application was found. The decrease in the photochemical efficiency of PSII photochemistry in the light adapted state and the big decrease in photochemical quenching, indicate that OMW resulted in diminished reoxidation of Q(A)(-) and started to inactivate the reaction centers of PSII. The OMW supply on soil and sand, resulted in leaf water stress and lesser water use efficiency. Plants treated with high OMW concentration, produced fewer but bigger tomatoes as compared to plants treated with lower OMW concentration. Generally, fruit yield and nutritional value was inhibited under OMW application.

  5. Characterization and quantification of phenolic compounds in four tomato (Lycopersicon esculentum L.) farmers' varieties in northeastern Portugal homegardens.

    Science.gov (United States)

    Barros, Lillian; Dueñas, Montserrat; Pinela, José; Carvalho, Ana Maria; Buelga, Celestino Santos; Ferreira, Isabel C F R

    2012-09-01

    Tomato (Lycopersicon esculentum L.) is one of the most widely consumed fresh and processed vegetables in the world, and contains bioactive key components. Phenolic compounds are one of those components and, according to the present study, farmers' varieties of tomato cultivated in homegardens from the northeastern Portuguese region are a source of phenolic compounds, mainly phenolic acid derivatives. Using HPLC-DAD-ESI/MS, it was concluded that a cis p-coumaric acid derivative was the most abundant compound in yellow (Amarelo) and round (Batateiro) tomato varieties, while 4-O-caffeolyquinic acid was the most abundant in long (Comprido) and heart (Coração) varieties. The most abundant flavonoid was quercetin pentosylrutinoside in the four tomato varieties. Yellow tomato presented the highest levels of phenolic compounds (54.23 μg/g fw), including phenolic acids (43.30 μg/g fw) and flavonoids (10.93 μg/g fw). The phenolic compounds profile obtained for the studied varieties is different from other tomato varieties available in different countries, which is certainly related to genetic features, cultivation conditions, and handling and storage methods associated to each sample.

  6. Negative effects of fluoranthene on the ecophysiology of tomato plants (Lycopersicon esculentum Mill) Fluoranthene mists negatively affected tomato plants.

    Science.gov (United States)

    Oguntimehin, Ilemobayo; Eissa, Fawzy; Sakugawa, Hiroshi

    2010-02-01

    Cherry tomato plants (Lycopersicon esculentum Mill) were sprayed with fluoranthene and mixture of fluoranthene and mannitol solutions for 30d. The exposure was carried out in growth chambers in field conditions, and the air was filtered through charcoal filters to remove atmospheric contaminants. Plants were sprayed with 10microM fluoranthene as mist until they reached the fruiting stage, and the eco-physiological parameters were measured to determine the effects of the treatments. We measured CO(2) uptake and water vapour exchange, chlorophyll fluorescence, leaf pigment contents, visual symptoms and biomass allocation. Fluoranthene which was deposited as mist onto leaves negatively affected both growth and the quality of tomato plants, while other treatments did not. The photosynthetic rate measured at saturated irradiance was approximately 37% lower in fluoranthene-treated plants compared with the control group. Other variables, such as stomata conductance, the photochemical efficiency of PSII in the dark, Chl a, Chl b, and the total chlorophyll contents of the tomato leaves were significantly reduced in the fluoranthene-treated plants. Tomato plants treated with fluoranthene showed severe visible injury symptoms on the foliage during the exposure period. Mannitol (a reactive oxygen scavenger) mitigated effects of fluoranthene; thus, reactive oxygen species generated through fluoranthene may be responsible for the damaged tomato plants. It is possible for fluoranthene to decrease the aesthetic and hence the economic value of this valuable crop plant.

  7. Physiological and biochemical responses of fruit exocarp of tomato (Lycopersicon esculentum Mill.) mutants to natural photo-oxidative conditions.

    Science.gov (United States)

    Torres, Carolina A; Andrews, Preston K; Davies, Neal M

    2006-01-01

    Photo-oxidative stress was imposed under natural solar radiation on exposed and shaded sections of detached fruit of immature green tomato (Lycopersicon esculentum Miller = Solanum lycopersicum L.) mutants (anthocyanin absent, beta-carotene, Delta, and high pigment-1) and their nearly isogenic parents ('Ailsa Craig' and 'Rutgers'). After 5 h exposure to high solar irradiance, either with or without ultraviolet (UV) radiation, surface colour changes, pigment composition, photosynthetic efficiency, antioxidant metabolites and enzyme activities, and selected flavonoids and antioxidant proteins in exocarp tissue were evaluated. The imposed photo-oxidative stress reproduced the symptoms observed on attached fruit. Both high temperature and solar irradiance caused fruit surface discoloration with faster degradation of chlorophyll (Chl) than carotenoids (Car), leading to an increase in the Car/Chl ratio. Surface bleaching was mostly caused by visible light, whereas elevated temperatures were mostly responsible for the inactivation of photosynthesis, measured as decreased F(v)/F(m). Ascorbate, glutathione, and total soluble protein concentrations decreased in the exocarp as the duration of exposure increased. Specific activities of superoxide dismutase, ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase (MDHAR), glutathione reductase (GR), and catalase increased with exposure, suggesting that these proteins were conserved during the imposed stress. GR protein expression remained stable during the imposed stress, whereas, MDHAR protein expression increased. Quercetin and kaempferol concentrations increased rapidly upon exposure, but not to UV radiation, suggesting rapid photo-protection in response to visible light; however, naringenin synthesis was not induced. The apparent increased tolerance of hp-1 fruit is discussed.

  8. Characterization of endophytic Bacillus strains from tomato plants (Lycopersicon esculentum) displaying antifungal activity against Botrytis cinerea Pers.

    Science.gov (United States)

    Kefi, Asma; Ben Slimene, Imen; Karkouch, Ines; Rihouey, Christophe; Azaeiz, Sana; Bejaoui, Marwa; Belaid, Rania; Cosette, Pascal; Jouenne, Thierry; Limam, Ferid

    2015-12-01

    Eighty endophytic bacteria were isolated from healthy tissues of roots, stems, leaves and fruits of tomato plants (Lycopersicon esculentum). Four strains, named BL1, BT5, BR8 and BF11 were selected for their antagonism against Botrytis cinerea, a phytopathogenic fungus responsible of gray mold in several important crops, with growth inhibitory activity ranging from 27 to 53%. Morphological, biochemical, and molecular parameters as 16S rDNA sequencing demonstrated that the selected bacterial strains were related to Bacillus species which are known to produce and secrete a lot of lipopeptides with strong inhibitory effect against pathogen mycelial growth. Electrospray mass spectrometry analysis showed that these strains produced heterogeneous mixture of antibiotics belonging to fengycin and surfactin for BL1 and BT5, to iturin and surfactin for BR8, to bacillomycin D, fengycin and surfactin for BF11. Furthermore, these bacteria exhibited biocontrol potential by reducing the disease severity when tested on detached leaflets. Based on their antifungal activity against Botrytis cinerea, these strains could be used for biological control of plant diseases.

  9. RESISTÊNCIA DE TOMATEIRO (Lycopersicon esculentum AO PATÓGENO Alternaria solani RESISTANCE OF TOMATO (Lycopersicon esculentum TO Alternaria solani PATHOGEN

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    Wilson Ferreira de Oliveira

    2007-09-01

    Full Text Available

    O objetivo deste trabalho foi avaliar níveis de resistência de variedades comerciais de tomateiro ao patógeno Alternaria solani. Os genótipos são pertencentes ao banco de germoplasma do CNPH-Embrapa, compreendendo híbridos F1, que foram avaliados em condições de campo. Os ensaios foram conduzidos nos campos experimentais da Escola de Agronomia e Engenharia de Alimentos da Universidade Federal de Goiás, em Goiânia-GO, a uma altitude de 730 m, latitude de 16° 41'S e longitude de 49° 17'W. As avaliações foram realizadas aos 36, 43, 50, 57, 64, 71, 78 e 85 dias após o transplantio, combinando-se escala diagramática e de notas. As variedades Ohio 4013 e CNPH 738 foram as mais resistentes entre os materiais tutorados. Entre os materiais rasteiros os mais resistentes foram o híbrido F1 Hawaii 7998 x Monense e Rotam 4. O genótipo Ohio 4013 e híbrido F1 Hawaii 7998 x Monense foram os que mais se destacaram para resistência a este patógeno, podendo ser indicados para futuros programas de melhoramento.

    PALAVRAS-CHAVE: Tomateiro; resistência; Alternaria solani.

    The main objective of this study was to test levels of resistance of commercial varieties, genotypes belonging to the germoplasm bank of CNPH-Embrapa and hibrids F1 of tomat to Alternaria solani, in field condition. The trials were conduced at the experimental fields of the Federal University of Goiás, Goiânia-GO, Brasil. It is located at an altitude of 730 m, latitude of 16° 41'S and longitude of 49° 17'W. The evaluations were accomplished at the 36, 43, 50, 57, 64, 71, 78 and 85 days after the seedlings planting, combining diagramatic scale and grades. Among indeterminate cultivars Ohio 4013 and CNPH 738 were the most resistants. Among determined ones the most

  10. Efectos genéticos y heterosis de tomate (Lycopersicon esculentum Mill. en campo e invernadero para rendimiento y calidad Genetic effects and heterosis in tomato (Lycopersicon esculentum Mill. in field and greenhouse for yield and quality

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    David Sánchez Aspeytia

    Full Text Available El cultivo de tomate (Lycopersicon esculentum Mill. ocupa el segundo lugar de importancia en México, por su gran producción de 2.22 millones de toneladas que alcanzo en el año 2007, con un rendimiento promedio de 35.54 t ha-1. Para la región noreste de México este cultivo representa una alternativa agrícola bajo condiciones de agricultura protegida debido a la poca disponibilidad de agua y a las temperaturas extremas que llegan a ser hasta 48 ºC en verano. El conocimiento de la habilidad combinatoria general y específica de materiales genéticos es esencial para lograr diversos objetivos en un programa de mejoramiento tales como: desarrollar híbridos y variedades, incrementar variabilidad genética y evitar erosión genética. El objetivo de la presente investigación fue evaluar los efectos genéticos de cuatro progenitores y seis cruzas directas posibles, evaluados en campo e invernadero bajo el análisis de variables de rendimiento y de calidad. En el análisis de varianza combinado hubo diferencias altamente significativas (p≤ 0.01 entre ambientes para peso promedio de fruto y rendimiento así como diferencias significativas (p≤ 0.05 para días a primer corte, donde las condiciones ambientales de cada localidad fueron diferentes para los genotipos en general, incluyendo progenitores e híbridos, peso promedio de fruto y rendimiento, indicándonos que los genotipos se comportaron diferente y que los híbridos difirieron en su comportamiento debido a la diversidad genética de los progenitores.Tomato cultivation (Lycopersicon esculentum Mill. ranks second place of importance in Mexico, for its big production of 2.22 million tons that reached in year 2007, with an average yield of 35.54 t ha-1. For northeast region of Mexico this crop represents an agricultural alternative under protected agriculture conditions due to little water availability and to extreme temperatures that rise up to 48 ºC in summer. Knowledge of general and

  11. Absisik asit (ABA) uygulamalarının domates (Lycopersicon esculentum Mill.) Fidelerinde bazı fizyolojik ve morfolojik özellikler ile kök gelişim değerlerine etkisi

    OpenAIRE

    2011-01-01

           Çalışma ile farklı doz ve sürelerde uygulanan absisik asit (ABA)'in domates (Lycopersicon esculentum Mill.) fidelerde oluşturduğu stresin; fizyolojik, morfolojik ve gelişim parametrelerine etkisi tespit edilmiş, ABA'nın domates fide yetiştiriciliğinde kullanılabilecek optimum doz ve uygulama süresi belirlenmiştir. Lycopersicon esculentum cv....

  12. EVALUACIÓN DE CARACTERÍSTICAS DE INTERÉS AGRONÓMICO DE SIETE GENOTIPOS NATIVOS DE JITOMATE (Lycopersicon esculentum Mill.) CULTIVADOS EN HIDROPONÍA

    OpenAIRE

    2012-01-01

    México es considerado el centro de domesticación del jitomate (Lycopersicon esculentum Mill.). Sin embargo, existe escasa información que permita el aprovechamiento de las formas nativas de esta especie. El objetivo de la presente investigación fue evaluar algunas características de interés agronómico de siete genotipos provenientes de los estados de Guerrero y Puebla, cultivados en invernadero e hidroponía, y compararlos con un híbrido comercial de jitomate cherry (H-790). Se evaluaron día...

  13. Effects of Different Sources of Nutrition on Quantitative and Qualitative Characteristics of Lycopersicon esculentum under Ecological Cropping System

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    M.B. Amiri

    2016-02-01

    Full Text Available Introduction: Increasing usage of chemical fertilizers imposes irreparable damages to the environment. Disadvantages of chemical fertilizers has led to more attention to the application of organic fertilizers and manures. The use of organic fertilizers and livestock, especially in nutrient poor soils, it is necessary to maintain soil quality. Plant growth promoting rhizobacteria (PGPR occupy the rhizosphere of many plant species and have beneficial effects on the host plant. They may influence the plant in a direct or indirect manner. A direct mechanism would be to increase plant growth by supplying the plant with nutrients and hormones. Indirect mechanisms on the otherhand, include, reduced susceptibility to diseases, and activing a form of defese referred to as induces systematic resistance. Examples of bacteria which have been found to enhance plant growth, include Pseudomonas, Enterobacter and Arthrobacter. Biofertilizers contain organic compounds that increase soil fertility either directly or as a result of their decay (9, 10. Tomato (Lycopersicon esculentum L. belongs to the nightshade family, Solanaceae. The plant typically grow 1-3 meters in height and a weak stem. It is a perennial in its native habitat, although often grown outdoors in temperate climates as an annual. An average common tomato weighs approximately 100 grams. Tomatoes contain the carotene lycopene, one of the most powerful natural antioxidants. In some studies, lycopene, especially in cooked tomatoes, has been found to help prevent prostate cancer. Lycopene has also been shown to improve the skin’s ability to protect against harmful UV rays. Tomatoes might help in managing human neurodegenerative diseases. The lycopene has no effect on the risk of developing diabetes, but may help relieve the oxidative stress of people who already have diabetes. The purpose of this study was the possibility of replacing chemical fertilizers with biofertilizers, reducing production

  14. Aftereffect conditions of prolonged space flight on physiological and biochemical processes and plant resistance Lycopersicon esculentum Mill. to pathogens

    Science.gov (United States)

    Mishchenko, Lidiya

    2016-07-01

    Tomatoes (Lycopersicon esculentum Mill.) - one of the most popular vegetables in Ukraine, they are a valuable product of therapeutic and dietetic foods because they contain a significant amount of nutrients and essential to the human body minerals and vitamins, but by the content of carotenoids - lycopene and β-carotene - is a powerful antioxidant. Therefore, tomato plants can be used successfully to astronauts on long space flights. We aftereffect was studied factors of space flight on the variety of tomato seeds Mir-1, which lasted (6 years) were on an orbital space station "Mir". Then, also after long-term storage in 2011, seeds were sown in the laboratory and received seedlings grown in field conditions Kiev region. The resulting seeds of the tomato crop in 2011 ("Space" and still) we used in our subsequent field studies in Kyiv and Poltava regions. We have previously shown that the "space" seeds had shown in 2011-2012 increased resistance to viruses PVY and PVM natural infectious background. Therefore, it is necessary continue the investigation and started to observe in future years, including 2015 and to analyze the results obtained. Because plants grown constantly in the field natural infectious background, there was a high probability of their defeat pathogens of different nature, including viruses. The works of many authors proved reduce the concentration of carotene and lycopene in tomatoes with the defeat of viruses (Raithak, 2012). In addition, the control plants were observed symptoms of such that is a viral infection, namely in 2011 - leaves curl in 2012 - except leaves curl and even mosaics. The research results were confirmed in 2013, namely on the plants of "space" seed no symptoms of, and in control - detection of potato virus Y (method RT-PCR) and symptoms of leaf curl and mosaic. During the bearing samples were taken leaves of the options and experiment conducted determination of photosynthetic pigments. It should be emphasized that in plant

  15. Effect of 1-methylcyclopropene post-harvest treatment on ripening process in cherry tomato fruit (Lycopersicon esculentum var. cerasiforme).

    Science.gov (United States)

    Opiyo, Arnold M; Ying, Tie-Jin

    2005-02-01

    The responses of cherry tomato (Lycopersicon esculentum var. cerasiforme) fruits to post-harvest treatment with 1-MCP were investigated. The maturity stage at which 1-MCP application is most effective in delaying the ripening process was determined, and then the effects of different concentrations (0, 0.035, 0.07 and 0.11 microL/L) of 1-MCP on ethylene production, fruit softening, chlorophyll, lycopene and carotenoids contents of mature green (MG) cherry tomato fruits were assessed. 1-MCP at 0.07 and 0.11 microL/L reduced fruit C(2)H(4) production, delayed the C(2)H(4) peak at ambient temperature. Although 1-MCP at 0.035 microL/L was effective in retarding fruit ripening, it did not suppress endogenous ethylene production. Fruit softening was suppressed by 1-MCP, but its initiation was not affected by 1-MCP. The rate of chlorophyll degradation and its pattern of change with time, and the initiation of lycopene biosynthesis as well as its accumulation were all affected by 1-MCP, but only the accumulation of carotenoids was suppressed. Accumulation of lycopene and carotenoids was almost permanently hampered by 1-MCP at 0.07 microL/L or higher concentrations, and fruit color could not reach the control level even 2 weeks after 1-MCP treatment, indicating the close association of the metabolism of these pigments with ethylene perception. Since the concentration of 0.11 microL/L of 1-MCP was so high that it did not elicit additional response very much than 0.07 microL/L, these concentrations were considered to be practically effective concentrations for cherry tomato at MG stage. The effective 1-MCP concentrations might provide a useful reference to the levels of ethylene receptors as well as ethylene sensitivity in a specific fruit at given development stage.

  16. Effect of Simulated Acid Rain on the Germination, Growth, Elements, Protein and Photosynthetic Pigments Contents in Tomato (Lycopersicon esculentum

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    M. Askary

    2014-04-01

    Full Text Available Uncontrolled use of fossil fuels in industries and the transport sector has led to an increase in concentrations of gaseous pollutants such as sulphur dioxide (SO2, nitrogen dioxide (NO2 and their derivatives and ozone (O3. In addition to dry and wet deposition of these gases has been the major route of influx in ionic form into the ecosystem. This investigation was evaluated the effects of simulated acid rain (SAR with different pH (6.8 as control, 6.5, 6, 5.5, 5, 4.5, 4, 3.5, 3 and 2.5 on germination, growth, elements, protein, photosynthetic pigments contents of Lycopersicon esculentum in hydroponic culture. Experiments were conducted at research laboratory of arak university in summer of 1391. Results were showed that from pH=6.8 until pH=5/5 significantly increased P and K and protein content, root and shoot dry and wet weight. SAR exposure with high acidity (pH=5/5 until pH=2.5 significantly suppressed germination, growth index, measured elements as P and K, protein and photosynthetic pigments, while significant increased sulphur contect from 150% to 550% compared to controls. Maximal amounts sulphur were measured in pH=2/5. Acid rain in low pH were decrease plant growth and make protein and incearsed sulphur content in leaf. As regards, low acidity promoted the growth of tomato plants and high acidity inhibit, Therefore, it is recommended that tomato plants cultures in soils with low acidity.

  17. Hypoglycaemic Effects of Dietary Intake of Ripe and Unripe Lycopersicon esculentum (Tomatoes on Streptozotocin-Induced Diabetes Mellitus in Rats

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    A. M. Akinnuga

    2010-01-01

    Full Text Available Ripe and unripe tomatoes have been implicated in prevention of chronic diseases such as cancer probably due to their antioxidant, antibiotic, antifungal and anti-inflammatory properties. Problem statement: This study was designed to investigate whether ripe and unripe tomatoes will have hypoglycaemic effect in a chronic disease such as diabetes mellitus that has been characterized with hyperglycaemia. Approach: Twenty albino Wistar rats were divided into 4 groups (2 control and 2 test groups of 5 rats each. The normal and diabetic control groups were given citrate buffer (intraperitoneally and normal rat chow and 65 mg kg-1 streptozotocin (intraperitoneally and normal rat chow respectively. The test groups were given 65 mg kg-1 streptozotocin via intraperitoneal route and either a mixture of ripe or unripe tomato and normal rat chow. In all groups, the blood samples were obtained at the tail vein of the animals and the fasting blood glucose level were monitored and determined on the 1st, 3rd and 14th day of consumption of different feed combinations. Results: There was significant difference in blood glucose level in animals fed on ripe and unripe Lycopersicon esculentum (tomato compared to the normal and diabetic control groups on the 3rd and 14th day without significant difference on the 1st day. Conclusion: Both high-lycopene ripe tomato and high-tomatine unripe tomato have hypoglycaemic effect in diabetic mellitus at short period of dietary intake therefore this suggest that consumers may benefit by not only eating high-lycopene ripe tomatoes, but also high-tomatine unripe tomatoes.

  18. Effect of Different Doses of NK Chemical Fertilizers and Compost on Growth and Yield Attributes of Tomato (Lycopersicon esculentum Mill.

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    Thayamini Harold Seran

    2016-07-01

    Full Text Available This study was conducted to evaluate the effect of NK chemical fertilizers in combination with compost on the growth and yield attributes of tomato (Lycopersicon esculentum Mill.. The pot experiment was done in a complete randomized block design possessing eight treatments with four replicates. Fruit weight, pulp weight, seed weight, total soluble solid, leaf area and dry weights of plat parts were taken and fruit yield was calculated. All the collected data were subjected to statistical analysis. The results revealed that there were significant differences in fruit and seed weights, total soluble solid, pulp weight, 100 seed weight, pulp consistency, leaf area and crop residue. In these parameters, higher mean values were recorded in chemical fertilizers (7.5 g N + 6 g K2O + 15 g P2O5 per m2 with compost (2 kg per m2 than those in the chemical fertilizers applied alone (9.0 g N + 8 g K2O + 15 g P2O5 per m2as standard control. Total soluble solid and fruit yield were 5.73 obrix and 3.21 kg/m2 respectively in the chemical fertilizers with compost (7.5 g N + 6 g K2O + 15 g P2O5 +2 kg compost per m2 treated plants and these were statically on par with the standard control which gave 4.36 obrix total soluble solid and 3.05 kg/m2 fruit yield. The result could be concluded that application of chemical fertilizers (7.5 g N + 6 g K2O + 15 g P2O5 per m2 with compost (2 kg per m2 could increase the total soluble solid and fruit yield as well as reduce the usage of chemical fertilizers in tomato cultivation over the standard control in sandy regosol.

  19. Efficiency of local Indonesia honey bees (Apis cerana L.) and stingless bee (Trigona iridipennis) on tomato (Lycopersicon esculentum Mill.) pollination.

    Science.gov (United States)

    Putra, Ramadhani Eka; Kinasih, Ida

    2014-01-01

    Tomato (Lycopersicon esculentum Mill.) is considered as one of major agricultural commodity of Indonesia farming. However, monthly production is unstable due to lack of pollination services. Common pollinator agent of tomatoes is bumblebees which is unsuitable for tropical climate of Indonesia and the possibility of alteration of local wild plant interaction with their pollinator. Indonesia is rich with wild bees and some of the species already domesticated for years with prospect as pollinating agent for tomatoes. This research aimed to assess the efficiency of local honey bee (Apis cerana L.) and stingless bee (Trigona iridipennis), as pollinator of tomato. During this research, total visitation rate and total numbers of pollinated flowers by honey bee and stingless bee were compared between them with bagged flowers as control. Total fruit production, average weight and size also measured in order to correlated pollination efficiency with quantity and quality of fruit produced. Result of this research showed that A. cerana has slightly higher rate of visitation (p>0.05) and significantly shorter handling time (p tomato flowers. However, honey bee pollinated tomato flowers more efficient pollinator than stingless bee (80.3 and 70.2% efficiency, respectively; p tomatoes were similar (p>0.05). Based on the results, it is concluded that the use of Apis cerana and Trigona spp., for pollinating tomatoes in tropical climates could be an alternative to the use of non-native Apis mellifera and bumblebees (Bombus spp.). However, more researches are needed to evaluate the cost/benefit on large-scale farming and greenhouse pollination using both bees against other bee species and pollination methods.

  20. The effect of the lenght of storage on the amount of lycopene in the fruits of tomato (Lycopersicon esculentum L.

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    Anton Uher

    2008-01-01

    Full Text Available We focused on tomatoes for industrial processing due to its economical importance for its lycopene content. The objective of our research is to find the variation of lycopene content in tomato fruits depending upon the length of after harvest storage and thermic treatment, which is inevitable when being industrialy processed. From the point of view of nutritional qualities the most relevant contentual substance of tomatos are carotenoids, included lycopene.At average for tree following experimental years we learnt significant differences regarding the content of lycopene and the length of storage of tomato fruits. Immediately after the harvest and proccesing tomato fruits contained, at average for tree years, 103.24 mg of lycopene. After 14 days the content of lycopene declined to 46.76 mg . kg−1 of fresh mass. After 30 days the average value dropped to 29.26 mg . kg−1. This fact comfirms that boiling respectively thermic treatment increases the content of lycopene in tomato fruits, particulary in our experiment to the value 83.33 mg . kg−1. At varieties Ladislav, Peto 86, Prémium, Salus the content of lycopene has even risen in comparison with its content up to 48 hours after the harvest. Tomato (Lycopersicon esculentum L. belongs to the most signifficant vegetable varieties either for its exploitation in processing industry as well as for its nutritional value with extraordinary beneficial effect for human organism.Although the content of lycopene is genetically stable attribute, its content in our experiment ranged from 45.39 mg . kg−1 (Prémium variety to 77.98 mg . kg−1 (Zámčan variety, which are significant differences.

  1. NUTRIÇÃO DO TOMATEIRO (Lycopersicon esculentum EM FUNÇÃO DE DOSES DE FERTILIZANTES ORGÂNICOS

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    José André Custódio da Silva

    2009-01-01

    Full Text Available The study aimed to evaluate the effect of two doses of organic material in the nutrition of tomato (Lycopersicon esculentum, cv Santa Cruz, grown in pots in an Oxisol Ultisol Distrocoeso the municipality of Rio Largo - AL. The design was in randomized blocks in factorial 2 X 4 (two and four fertilizer doses with 5 replicates. The experimental unit consisted of a vessel with capacity for 12 dm3 containing 15 kg of soil. The fertilizer materials were: 1 fertilizer - manure from corral + chicken manure + fertilizer and the filter cake of 2 - to corral manure + chicken manure + sugar cane bagasse, in doses 0, 5, 10 and 15 t ha-1. The applications were split in the following way: 10% at the time of transplantation, and 15% at 20, 34, 48, 62, 76 and 90 days after transplanting (DAT in coverage. The seedlings were produced in the tomato seed, on March 10, 2008, in polystyrene trays with 128 cells, after transplantation and were selected for the vessel on April 02, 2008, leaving two plants per pot . The plants were conducted in mentoring and single stem, and where, therefore, all cultural and treatment plant. There was a collection of leaves 4 + from the apex of the plant, to assess nutritional status at the beginning of flowering. Among the nutrients, the nitrogen was absorbed the most, followed by: potassium, calcium, sulfur, magnesium and phosphorus, whereas among the micronutrients, in increasing order of absorption was the following: boron, zinc, copper, manganese and iron. According to analysis of variance and regression analysis of the fertilizer did not affect the levels of nutrients in the plant, with only difference between the doses.

  2. HIGH TEMPERATURE EFFECT ON TOMATO (Lycopersicon esculentum PIGMENT AND PROTEIN CONTENT AND CELLULAR VIABILITY

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    Daymi Camejo

    2001-01-01

    Full Text Available Cultivares de tomate con diferente sensibilidad a las altas temperaturas (Campbell-28 y Amalia y el tipo silvestre Nagcarlang, fueron expuestos a diferentes condiciones de estrés (45oC-dos horas de exposición, 45oC-tres horas de exposición y 25oC como control. Los contenidos de clorofila “a”, “b” y total fueron determinados, así como los de carotenoides y proteinas totales solubles. La relación clorofila “a”/“b” y clorofila/carotenoides fue estimada a partir de los resultados obtenidos. Las variables evaluadas fueron modificadas según el cultivar y la condición de estrés. La selección de Nagcarlang como modelo de termotolerancia permite establecer que variables tales como: relación de clorofila “a”/“b”, clorofila/carotenoides, contenido de proteínas totales solubles y viabilidad celular, pudieran ser útiles para describir cultivares tolerantes a las altas temperaturas, dependiendo del tiempo de exposición al estrés.

  3. Yield and quality of tomato (Lycopersicon esculentum Mill. fruit harvested from plants grown in mulched soil

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    Joanna Majkowska-Gadomska

    2012-12-01

    Full Text Available A study investigating the yield of field-grown tomatoes was conducted in 2007–2009 in the Garden of the Research and Experimental Station of the University of Warmia and Mazury in Olsztyn. The experimental materials comprised two tomato cultivars, 'Bawole Serce' and 'Złoty Ożarowski'. Tomato plants were grown in bare soil and in soil mulched with black non-woven PP 50 fabric. Cultivar selection had a significant effect only on average early yield of 'Bawole Serce'. The highest average early yield for three years of the study was recorded for 'Bawole Serce' grown in mulched soil, while the lowest one for 'Złoty Ożarowski' grown in mulched plots. Tomato marketable yield was significantly higher in both treatments where 'Bawole Serce' plants were grown. Fruits harvested from tomato plants 'Złoty Ożarowski' had a higher dry matter content. Soil mulching significantly increased the dry matter content of 'Złoty Ożarowski' tomato fruit. The experimental factors had no influence on the concentrations of L-ascorbic acid, total sugars, and organic acids. Nitrate levels in tomato fruit were within permissible limits, and they were significantly affected by the cultivation method and the method x cultivar interaction. Nitrate accumulation was reduced in tomato plants 'Złoty Ożarowski' grown in mulched soil.

  4. Influence of modified atmosphere and ethylene levels on quality attributes of fresh tomatoes (Lycopersicon esculentum Mill.).

    Science.gov (United States)

    Domínguez, Irene; Lafuente, María T; Hernández-Muñoz, Pilar; Gavara, Rafael

    2016-10-15

    Controlling storage atmosphere is a key factor for delaying postharvest fruit quality loss. The objective of this study is to evaluate its influence on physico-chemical, sensorial and nutritional quality attributes of two tomato fruit cultivars (Delizia and Pitenza) that respectively have a short- and long-storage life. To that end, the effect of two types of bags with different gas permeability, combined or not with an ethylene sorbent, on tomato organoleptic and nutritional properties were compared during fruit storage at 13°C. CO2 and O2 were critical factors for controlling tomato postharvest behaviour. Weight loss, firmness, color and visual quality were only affected by bag permeability just as total sugar content and acidity for Pitenza tomatoes. (trans)-2-Hexenal also appears to be related with CO2 and O2 levels. Lycopene, total phenols (TP) and ascorbic acid (AA) contents were also affected by the packaging form and the storage length. Ethylene removal in combination with MAP led to a higher content in TP and AA in the short-life tomato cultivar.

  5. Identification of QTLs for early blight ( Alternaria solani) resistance in tomato using backcross populations of a Lycopersicon esculentum x L. hirsutum cross.

    Science.gov (United States)

    Foolad, R.; Zhang, P.; Khan, A. A.; Niño-Liu, D.; Lin, Y.

    2002-05-01

    Most commercial cultivars of tomato, Lycopersicon esculentum Mill., are susceptible to early blight (EB), a devastating fungal ( Alternaria solani Sorauer) disease of tomato in the northern and eastern parts of the U.S. and elsewhere in the world. The disease causes plant defoliation, which reduces yield and fruit quality, and contributes to significant crop loss. Sources of resistance have been identified within related wild species of tomato. The purpose of this study was to identify and validate quantitative trait loci (QTLs) for EB resistance in backcross populations of a cross between a susceptible tomato breeding line (NC84173; maternal and recurrent parent) and a resistant Lycopersicon hirsutum Humb. and Bonpl. accession (PI126445). Sixteen hundred BC(1) plants were grown to maturity in a field in 1998. Plants that were self-incompatible, indeterminant in growth habit, and/or extremely late in maturity, were discarded in order to eliminate confounding effects of these factors on disease evaluation, QTL mapping, and future breeding research. The remaining 145 plants (referred to as the BC(1) population) were genotyped for 141 restriction fragment length polymorphism (RFLP) markers and 23 resistance gene analogs (RGAs), and a genetic linkage map was constructed. BC(1) plants were evaluated for disease symptoms throughout the season, and the area under the disease progress curve (AUDPC) and the final percent defoliation (disease severity) were determined for each plant. BC(1) plants were self-pollinated and produced BC(1)S(1) seed. The BC(1)S(1) population, consisting of 145 BC(1)S(1) families, was grown and evaluated for disease symptoms in replicated field trials in two subsequent years (1999 and 2000) and AUDPC and/or final percent defoliation were determined for each family in each year. Two QTL mapping approaches, simple interval mapping (SIM) and composite interval mapping (CIM), were used to identify QTLs for EB resistance in the BC(1) and BC(1)S(1

  6. Polyuronides in Avocado (Persea americana) and Tomato (Lycopersicon esculentum) Fruits Exhibit Markedly Different Patterns of Molecular Weight Downshifts during Ripening.

    Science.gov (United States)

    Huber, D. J.; O'Donoghue, E. M.

    1993-01-01

    Avocado (Persea americana) fruit experience a rapid and extensive loss of firmness during ripening. In this study, we examined whether the chelator solubility and molecular weight of avocado polyuronides paralleled the accumulation of polygalacturonase (PG) activity and loss in fruit firmness. Polyuronides were derived from ethanolic precipitates of avocado mesocarp prepared using a procedure to rapidly inactivate endogenous enzymes. During ripening, chelator (cyclohexane-trans-1,2-diamine tetraacetic acid [CDTA])-soluble polyuronides increased from approximately 30 to 40 [mu]g of galacturonic acid equivalents (mg alcohol-insoluble solids)-1 in preripe fruit to 150 to 170 [mu]g mg-1 in postclimacteric fruit. In preripe fruit, chelator-extractable polyuronides were of high molecular weight and were partially excluded from Sepharose CL- 2B-300 gel filtration media. Avocado polyuronides exhibited marked downshifts in molecular weight during ripening. At the postclimacteric stage, nearly all chelator-extractable polyuronides, which constituted from 75 to 90% of total cell wall uronic acid content, eluted near the total volume of the filtration media. Rechromatography of low molecular weight polyuronides on Bio-Gel P-4 disclosed that oligomeric uronic acids are produced in vivo during avocado ripening. The gel filtration behavior and pattern of depolymerization of avocado polyuronides were not influenced by the polyuronide extraction protocol (imidazole versus CDTA) or by chromatographic conditions designed to minimize interpolymeric aggregation. Polyuronides from ripening tomato (Lycopersicon esculentum) fruit extracted and chromatographed under conditions identical with those used for avocado polyuronides exhibited markedly less rapid and less extensive downshifts in molecular weight during the transition from mature-green to fully ripe. Even during a 9-d period beyond the fully ripe stage, tomato fruit polyuronides exhibited limited additional depolymerization and

  7. Interaction of Polyamines, Abscisic Acid, Nitric Oxide, and Hydrogen Peroxide under Chilling Stress in Tomato (Lycopersicon esculentum Mill.) Seedlings.

    Science.gov (United States)

    Diao, Qiannan; Song, Yongjun; Shi, Dongmei; Qi, Hongyan

    2017-01-01

    Polyamines (PAs) play a vital role in the responses of higher plants to abiotic stresses. However, only a limited number of studies have examined the interplay between PAs and signal molecules. The aim of this study was to elucidate the cross-talk among PAs, abscisic acid (ABA), nitric oxide (NO), and hydrogen peroxide (H2O2) under chilling stress conditions using tomato seedlings [(Lycopersicon esculentum Mill.) cv. Moneymaker]. The study showed that during chilling stress (4°C; 0, 12, and 24 h), the application of spermidine (Spd) and spermine (Spm) elevated NO and H2O2 levels, enhanced nitrite reductase (NR), nitric oxide synthase (NOS)-like, and polyamine oxidase activities, and upregulated LeNR relative expression, but did not influence LeNOS1 expression. In contrast, putrescine (Put) treatment had no obvious impact. During the recovery period (25/15°C, 10 h), the above-mentioned parameters induced by the application of PAs were restored to their control levels. Seedlings pretreated with sodium nitroprusside (SNP, an NO donor) showed elevated Put and Spd levels throughout the treatment period, consistent with increased expression in leaves of genes encoding arginine decarboxylase (LeADC. LeADC1), ornithine decarboxylase (LeODC), and Spd synthase (LeSPDS) expressions in tomato leaves throughout the treatment period. Under chilling stress, the Put content increased first, followed by a rise in the Spd content. Exogenously applied SNP did not increase the expression of genes encoding S-adenosylmethionine decarboxylase (LeSAMDC) and Spm synthase (LeSPMS), consistent with the observation that Spm levels remained constant under chilling stress and during the recovery period. In contrast, exogenous Put significantly increased the ABA content and the 9-cis-epoxycarotenoid dioxygenase (LeNCED1) transcript level. Treatment with ABA could alleviate the electrolyte leakage (EL) induced by D-Arg (an inhibitor of Put). Taken together, it is concluded that, under chilling

  8. Effect of Organic and Biological Fertilizers on Growth and Yield of Tomatoes (Lycopersicon esculentum Mill. and Bacterial Colonization

    Directory of Open Access Journals (Sweden)

    H. Makarian

    2016-02-01

    Full Text Available Introduction: In recent decades, excessive use of chemical fertilizers causes environmental problems such as water resource pollution and decrease in soil fertility. Organic matters are excellent sources of plant-available nutrients and their addition to soil could maintain high microbial populations and activities. In crop studies, Prabha et al. (2007 reported that there was excellent plant growth as well as yield in garlic plants that received vermicompost as nutrient in the field (28. Recent studies confirmed that, a number of bacterial species mostly associated with the plant rhizosphere, are found to be beneficial for plant growth, yield and crop quality. Therefore, the objective of this study is to investigate the growth promoting effects of organic and bio-fertilizers on tomato growth and yield. Materials and Methods: A factorial experiment in randomized complete block design with three replications was conducted at the College of Agricultural, University of Shahrood in 2011. Geographically, the site is located in Bastam (36° 25’E, 54° 58’N, 1349 m a.s.l..The climate of this region is semi-arid. Treatments included three levels of organic fertilizers: vermicompost (1300 kgha-1, cow manure (3350 kgha-1, and control, biological fertilizer in four levels (Pseudomonas putyda, Pseudomonas fluorescens, Azotobacter chrococcum and control. The bacterial suspension for each species was applied at a rate of 3 liters per hectare. Metribuzin herbicide (wettable 80% powder was used at a rate of 1000 gr. ha-1. Petopride No. 2' variety of tomato (Lycopersicon esculentum Mill. was used in the present experiment. At the time of harvesting, the plant characteristics namely lengths and diameter of stem, number and weight of fruit, weight of stem and leaf were also registered. Statistical analyses of data were performed with statistical software Mstatc. Significant differences between means refer to the probability level of 0.05 by LSD test. Results

  9. Osmotic adjustment and the growth response of seven vegetable crops following water-deficit stress. [Phaseolus vulgaris L. ; Beta vulgaris L. ; Abelmoschus esculentus; Pisum sativum L. ; Capsicum annuum L. ; Spinacia oleracea L. ; Lycopersicon esculentum Mill

    Energy Technology Data Exchange (ETDEWEB)

    Wullschleger, S.D. (Oak Ridge National Lab., TN (United States)); Oosterhuis, D.M. (Univ. of Arkansas, Fayetteville (United States))

    1991-09-01

    Growth-chamber studies were conducted to examine the ability of seven vegetable crops- Blue Lake beam (Phaseolus vulgaris L.) Detroit Dark Red beet (Beta vulgaris L.) Burgundy okra (Abelmoschus esculentus) (Moench), Little Marvel pea (Pisum sativum L), California Wonder bell pepper (Capsicum annuum L), New Zealand spinach (Spinacia oleracea L), and Beefsteak tomato (Lycopersicon esculentum Mill.) - to adjust osmotically in response to water-deficit stress. Water stress was imposed by withholding water for 3 days, and the adjustment of leaf and root osmotic potentials upon relief of the stress and rehydration were monitored with thermocouple psychrometers. Despite similar reductions in leaf water potential and stomatal conductance among the species studied reductions in lead water potential an stomatal conductance among the species, crop-specific differences were observed in leak and root osmotic adjustment. Leaf osmotic adjustment was observed for bean, pepper, and tomato following water-deficit stress. Root osmotic adjustment was significant in bean, okra, pea and tomato. Furthermore, differences in leaf and root osmotic adjustment were also observed among five tomato cultivars. Leaf osmotic adjustment was not associated with the maintenance of leaf growth following water-deficit stress, since leaf expansion of water-stressed bean and pepper, two species capable of osmotic adjustment, was similar to that of spinach, which exhibited no leaf osmotic adjustment.

  10. Acetylcholine suppresses shoot formation and callusing in leaf explants of in vitro raised seedlings of tomato, Lycopersicon esculentum Miller var. Pusa Ruby.

    Science.gov (United States)

    Bamel, Kiran; Gupta, Rajendra; Gupta, Shirish C

    2016-06-02

    We present experimental evidence to show that acetylcholine (ACh) causes decrease in shoot formation in leaf explants of tomato (Lycopersicon esculentum Miller var Pusa Ruby) when cultured on shoot regeneration medium. The optimum response was obtained at 10(-4) M ACh-enriched medium. ACh also causes decrease in percentage of cultures forming callus and reduces the callus mass. Inhibitors of enzymatic hydrolysis of ACh, neostigmine and physostigmine, also suppresses callogenesis and caulogenesis. On the other hand, the breakdown products of Ach, choline and acetate, do not alter the morphogenic response induced on the shoot regeneration medium. Neostigmine showed optimal reduction in shoot formation at 10(-5) M. The explants cultured on neostigmine augmented medium showed decline in the activity of ACh hydrolyzing enzyme acetylcholinesterase. ACh and neostigmine added together showed marked reduction in callus mass. These results strongly support the role of ACh as a natural regulator of morphogenesis in tomato plants.

  11. Evaluation of biological nematicides on the control of root-knot nematode (Meloidogyne incognita Chitwood at protected crops house in tomato (Lycopersicon esculentum Mill.

    Directory of Open Access Journals (Sweden)

    Pedro Fidel Fuentes Chaviano

    2011-06-01

    Full Text Available This research was carried out on Tomato (Lycopersicon esculentum Mill. variety HA 3105 at the protected crops houses in Empresa Azucarera Melanio Hernández of Sancti Spiritus, located at the southwest of Tuinucú, from November 2009 to May 2010 with the objective of evaluating the effectiveness of biological nematicides on the control of the root-knot nematode, Meloidogyne incognita Chitwood. Three treatments were used: Agrocelhone, biological nematicide HeberNem and HeberNem + Trichoderma. Biological nematicides showed better results than Agrocelhone regarding the length of the leaf, the height of the plant, the yield of tomato and the control of nematodes HeberNem showed the best results in the control of the nematode, decreasing the infestation from degree V to degree II.

  12. Factors affecting the production of seeds in fully fertile tomatoes (Lycopersicon esculentum L. Mill. and those showing a tendency to parthenocarpy

    Directory of Open Access Journals (Sweden)

    Barbara Gabara

    2014-01-01

    Full Text Available Comparative studies on the development of the female gametophyte, pollination and fertilization in two lines of Lycopersicon esculentum, Kholodostoykye (Kh, fertile and A33 (with a tendency to parthenocarpy have revealed that seed production is affected by disturbances in embryo sac formation but mainly by its degeneration after anthesis, which is especially visible in line A33. Moreover, delayed development of some embryo sacs and incomplete pollination due to various stigma levels seem to be responsible for the diminution of seed number in line A33. Deep fluorescence of numerous pollen grains as well as whole pollen tubes in 83.3 per cent of A33 stigmas and only 24.1 per cent in the Kh line points to the heterogeneity of pollen. This could be one more reason for reduced fertility. The results of application of plant growth regulators (auxin, PCIB which affect seed production in tomato of line A33 remain inconclusive.

  13. Modelo de producción de tomate Lycopersicon esculentum Mill usando como sustrato la mezcla cachaza-carbonilla en diferentes dosis de fertilización

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    Bruzón C. Serapio F.

    1996-12-01

    Full Text Available

    The industrial subproducts filter cake press and botton fly ash were used in mixtures (3:1 like sustratum for the tomato Lycopersicon esculentum Mill (Celebrity HF1 production. The trial constituted six treatments each one repeated three times. Half of treatments were sowed in the mixture and the other half in the soil, using three fertilization levels: 0, 300 and 600 kg/ha 18-18*-18. There were productions of 140, 158 and 178 ton/ha for the three fertilization levels using the filter press cake substate and 140, 143 and 160 t/ha tor the three fertilization levels in the soil. Such results are showing the excellent quality of the mixture filter cake press: coal dust (3:1 because of the similar results, which were obtained in one soil with excellent physics and chemical properties.

    Los subproductos industriales cachaza y carbonilla se utilizaron en mezcla (3:1 como sustrato para producción de tomate Lycopersicon esculentum Mili, Celebrity HF1. El experimento se diseñó en bloques completos al azar con arreglo factorial; constó de seis tratamientos y tres repeticiones cada uno, la mitad de los tratamientos se sembró en la mezcla y la otra mitad en suelo, utilizándose tres niveles de fertilización: 0, 300 y 600 kg/Ha 18-18-18. Se obtuvieron producciones de 140, 158 y 178 ton/ha de tomate para los tres niveles de fertilización en el sustrato cachaza y 140, 143 y 160 para los tres niveles de fertilización en el suelo, lo que demuestra la excelente calidad de la mezcla cachaza: carbonilla (3:1, al obtenerse resultados similares al suelo de excelentes propiedades físicas y químicas.

     

  14. Response of superoxide dismutase isoenzymes in tomato plants (Lycopersicon esculentum) during thermo-acclimation of the photosynthetic apparatus.

    Science.gov (United States)

    Camejo, Daymi; Martí, María del C; Nicolás, Emilio; Alarcón, Juan J; Jiménez, Ana; Sevilla, Francisca

    2007-11-01

    Seedlings of Lycopersicon esculentum Mill. var. Amalia were grown in a growth chamber under a photoperiod of 16 h light at 25 degrees C and 8 h dark at 20 degrees C. Five different treatments were applied to 30-day-old plants: Control treatment (plants maintained in the normal growth conditions throughout the experimental time), heat acclimation (plants exposed to 35 degrees C for 4 h in dark for 3 days), dark treatment (plants exposed to 25 degrees C for 4 h in dark for 3 days), heat acclimation plus heat shock (plants that previously received the heat acclimation treatment were exposed to 45 degrees C air temperature for 3 h in the light) and dark treatment plus heat shock (plants that previously received the dark treatment were exposed to 45 degrees C air temperature for 3 h in the light). Only the heat acclimation treatment increased the thermotolerance of the photosynthesis apparatus when the heat shock (45 degrees C) was imposed. In these plants, the CO(2) assimilation rate was not affected by heat shock and there was a slight and non-significant reduction in maximum carboxylation velocity of Rubisco (V(cmax)) and maximum electron transport rate contributing to Rubisco regeneration (J(max)). However, the plants exposed to dark treatment plus heat shock showed a significant reduction in the CO(2) assimilation rate and also in the values of V(cmax) and J(max). Chlorophyll fluorescence measurements showed increased thermotolerance in heat-acclimated plants. The values of maximum chlorophyll fluorescence (F(m)) were not modified by heat shock in these plants, while in the dark-treated plants that received the heat shock, the F(m) values were reduced, which provoked a significant reduction in the efficiency of photosystem II. A slight rise in the total superoxide dismutase (SOD) activity was found in the plants that had been subjected to both heat acclimation and heat shock, and this SOD activity was significantly higher than that found in the plants subjected to

  15. Lycopersicon esculentum Mill.

    African Journals Online (AJOL)

    HP

    2015-06-03

    Jun 3, 2015 ... clusters were monogenotypic and cluster I possessed highest number of genotypes numbering 25. Out ... be used to compare the genetic dis-similarity between different species ... order to maintain the native flavour and taste.

  16. Gamma radiation effects of Cobalt-60 on tomatoes (Lycopersicon esculentum Mill.) stored at room temperature and under refrigerated conditions; Efeito da radiacao gama do Cobalto-60 em tomates (Lycopersicon esculentum Mill.) armazenados em temperatura ambiente e refrigeracao

    Energy Technology Data Exchange (ETDEWEB)

    Penna, Raquel Medeiros de Almeida

    1994-07-01

    This work was carried out in two distinct experiments. Tomatoes (Lycopersicon esculentum Mill.) of the variety 'Santa Clara in the 'turning' stage (first experiment) and of the variety 'Santa Cruz' in the 'mature-green' stage (second experiment), were irradiated in a Cobalt-60 source with doses of 0.0; 0.5; 1.0; 1.5 e 2.0 kGy, and stored at room temperature (20{+-}2 deg C) and under refrigeration (9 deg C). The aim of the experiments was to verify the effects of the gamma-radiation on the extension of the market life of tomatoes stored under two different temperatures. The following types of analysis were carried out: physical (weight and skin resistance), chemical (brix, pH), sensorial (general appearance, external coloration and firmness) and organoleptic (aroma and flavor), during 30 days of storage, with four evaluations. At the end of the experiment microbiologic and microscopic analyses of histological sections were also performed. In both experiments, occurred a greater loss of weight in the irradiated tomatoes in relation to the controls proportional to the increase in the period of storage, but not to the increase of doses. This effect was more pronounced in fruits stored at room temperature. Concerning the resistance, the fruits of the first experiment showed that irradiation causes softening of the fruit skin. This softening was proportional to the increase of doses, in the 1, 10 e 30th day, and also the fruits irradiated with 1.0, 1.5 and 2.0 kGy showed a higher softening on both temperatures. In the second experiment, on both temperatures, the fruits showed an increase of skin resistance until the 10th day, which diminished until the 30th day. The content of soluble solids was altered by irradiation in the first experiment, and this resulted in a decrease of Brix proportional to the increase of dose. In the second experiment, the radiation induced a maximum Brix value at the 10th day in the fruits maintained under

  17. Activation of the cell cycle in tomato (Lycopersicon esculentum Mill.) seeds during osmoconditioning as related to temperature and oxygen

    NARCIS (Netherlands)

    Ozbingöl, N.; Corbineau, F.; Groot, S.P.C.; Bino, R.J.; Côme, D.

    1999-01-01

    Using flow cytometric analyses of the nuclear DNA content, we studied the effects of various conditions of osmopriming on the activation of the cell cycle in embryo root tips of tomato (Lycopersicon esculentum‘Elko’) seeds. In dry untreated seeds, 90.7% of the nuclei revealed 2C signals. Priming of

  18. Activation of the cell cycle in tomato (Lycopersicon esculentum Mill.) seeds during osmoconditioning as related to temperature and oxygen

    NARCIS (Netherlands)

    Ozbingöl, N.; Corbineau, F.; Groot, S.P.C.; Bino, R.J.; Côme, D.

    1999-01-01

    Using flow cytometric analyses of the nuclear DNA content, we studied the effects of various conditions of osmopriming on the activation of the cell cycle in embryo root tips of tomato (Lycopersicon esculentum‘Elko’) seeds. In dry untreated seeds, 90.7% of the nuclei revealed 2C signals. Priming of

  19. Métodos de remoção da mucilagem e qualidade fisiológica de sementes de tomate (Lycopersicon esculentum, Mill. Methods of mucilage remotion and physiological quality of tomato (Lycopersicon sculentum Mill. seeds

    Directory of Open Access Journals (Sweden)

    C. Cavariani

    1994-04-01

    Full Text Available Objetivando estudar diferentes métodos de remoção da mucilagem e a qualidade de sementes de tomate (Lycopersicon esculentum Mill., conduziu-se um experimento, em delineamento inteiramente casualizado com quatro repetições. Após extração manual, sementes e mucilagens foram submetidas aos seguintes tratamentos de remoção: lavagem imediata, fermentação por três dias, ácido clorídrico a 2,5% (25 ml/kg, durante 2 horas, ácido acético a 10% (25 ml/kg de material e hidróxido de amônia a 2,5% (25 ml/kg, durante 2 horas. Seguiu-se a avaliação da qualidade fisiológica das sementes através das determinações de porcentagem de germinação e vigor, pela porcentagem de emergência em areia, condutividade elétrica e pesos das matérias verde e seca de plântulas. Os resultados permitiram concluir que os métodos de remoção por lavagem imediata, fermentação, ácido clorídrico e ácido acético comercial não afetaram a qualidade fisiológica de sementes de tomate; a porcentagem de germinação não foi afetada pelos diferentes métodos de remoção e o método hidróxido de amônia prejudica o vigor das sementes.Different methods of mucilage remotion and quality of tomato (Lycopersicon esculentum Mill. seeds were studied, in a complete randomized design experiment with four replications. After manual extraction, seeds and slimes were submitted to the following remotion treatments: immediate washing, three-day fermentation, hydrochloric acid at 2,5% (25 mg/kg for 2 hrs., acetic acid at 10% (25 ml/kg of material, and ammonia hydroxide at 2,5% (25 ml/kg, for 2 hrs.. A physiological quality evaluation of seeds was carried out through the percentage of germination and vigor, percentage of in-sand emergence, electrical conductivity, and weight of fresh and dry matters of seedlings. The results showed that the methods of immediate washing, fermentation, commercial hydrochloric and acetic acids do not affect the physiological quality of

  20. PROTEÍNAS ANTIFÚNGICAS CONTRA P. INFESTANS EN LOS ESPACIOS INTERCELULARES DE HOJAS DE TOMATE (Lycopersicon esculentum cerasiforme POSIBLES DEFENSINAS DE PLANTAS

    Directory of Open Access Journals (Sweden)

    Luz Dary Riaño

    2008-03-01

    Full Text Available Se aislaron y caracterizaron parcialmente proteínas antifúngicas de los espacios intercelulares de hojas de tomate Lycopersicon esculentum cerasiforme, variedad que ha mostrado resistencia en campo a Phytophthora Infestans; se observó quedespués de inoculación con el patógeno dichas proteínas se acumularon sistémicamente en la planta. Las proteínas identificadasmostraron características de Defensinas de plantas, una nueva familia de proteínas con bajo peso molecular, carga positiva a pH fisiológico y actividad antifúngica evaluada in vitro contra P. infestans.Los análisis electroforéticos en geles de poliacrilamida con SDS-Tricina en condiciones reductoras y no reductoras, sugirieron que están asociadas en trímeros y tetrámeros y poseen pesos moleculares de 5,2 kDa.

  1. Antioxidant-enzyme reaction to the oxidative stress due to alpha-cypermethrin, chlorpyriphos, and pirimicarb in tomato (Lycopersicon esculentum Mill.).

    Science.gov (United States)

    Chahid, Karim; Laglaoui, Amin; Zantar, Said; Ennabili, Abdeslam

    2015-11-01

    Tomato (Lycopersicon esculentum Mill.) becomes one of the world's foremost vegetables, and its world production and consumption have increased fairly quickly. The capacity to induce oxidative stress in tomato plant, exposed to three xenobiotics such as alpha-cypermethrin, chlorpyriphos, and pirimicarb, was investigated by the evaluation of lipid peroxidation by measuring malondialdehyde (MDA) rate; also, we studied the response of tomato to this stress by assessing the response of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), glutathione-s-transferase (GST), and glutathione reductase (GR). The effect of the insecticides was observed using four concentrations (25, 50, 75, and 100%) for germinating seeds and only the recommended concentration in agriculture (100%) for growing plants. Our results show an important accumulation of MDA, demonstrating the increase of lipid peroxidation in consequence of the excessive reactive oxygen species (ROS) production due to insecticide treatment. In response to this oxidative stress in tomato seedlings and plants, the activities of antioxidant-enzyme system were generally enhanced. The electrophoretic analysis showed also the apparition of new isoenzymes as the case for CAT and POD.

  2. Effects of Crop Development on the Emission of Volatiles in Leaves of Lycopersicon esculentum and Its Inhibitory Activity to Botrytis cinerea and Fusarium oxysporum

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Volatiles emitted from the leaves of Lycopersicon esculentum at the two-, ten-leaf and anthesis periods were collected by a gas absorbing method and analyzed by gas chromatography (GC)-mass spectrometry. In total, 33 compounds of volatiles emitted from three developmental stage plants were separated and identified, and quantitatively analyzed by the internal standard addition method. All of the samples of volatile were found to be rich in monoterpenes and sesquiterpenes.β-phellandrene and caryophyllene predominated in the volatiles of the leaves of plants at the two- and ten-leaf stages.Furthermore, (E)-2-hexenal were the dominant components in the volatiles emitted from anthesis plants. The results of volatiles analyzed show that the compositions varied depending on the developmental stages. The volatiles emitted from crushed tomato leaves of plants at the anthesis stage had the most strongly inhibitory activity against the spore germination and hyphal growth of Botrytis cinerea and Fusarium oxysporum, followed by ten- and two-leaf plants. However, the activity of volatiles, emitted from the leaves of plants at the two-leaf stage, in inhibiting F. oxysporum was greater than B. cinerea.

  3. The role of cow dung and kitchen manure composts and their non-aerated compost teas in reducing the incidence of foliar diseases of Lycopersicon esculentum (Mill

    Directory of Open Access Journals (Sweden)

    A. Ngakou

    2014-06-01

    Full Text Available Compost teas are fermented watery extracts of composted materials used for their beneficial effect on plants. A study was conducted in the field to compare the efficacy of cow dung and kitchen manure composts and their derived non-aerated compost teas on disease symptoms expression and severity of Lycopersicon esculentum. The experimental layout was a complete randomised block design comprising six treatments, each of which was repeated three times: the negative control plot (Tm-; the positive control or fungicide plot (Tm+; the cow dung compost plot (Cpi; the kitchen manure compost plot (Cpii; the compost tea derived cow dung plot (Tci; and the compost tea derived kitchen manure plot (Tcii. Compost tea derived cow dung was revealed to be richer in elemental nutrients (N, P, K than compost tea from kitchen manure, and significantly (p < 0.0001 enhanced fruit yield per plant. Similarly, the two composts and their derived compost teas significantly (p < 0.0001 reduced the incidence and severity of disease symptoms compared to the controls, with the highest efficacy accounting for cow dung compost and compost tea. Although the non-aerated compost teas were not amended with micro-organisms, these results suggest that the two compost teas in use were rich enough in microbial pathogen antagonists, and therefore, are perceived as potential alternatives to synthetic chemical fungicides. Future work will attempt to identify these microbial antagonists with highly suppressive activity in the non-aerated compost teas.

  4. Polyhydroxyalcanoates of strains of Azospirillum spp. isolated of roots of Lycopersicon esculentum Mill. “tomato” and Oryza sativa L. “rice” in Lambayeque

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    Katty Baca

    2010-12-01

    Full Text Available In this work was determined the concentration of polyhydroxyalkanoates (PHAs of Azospirillum strains isolated from roots of Lycopersicon esculentum Mill "tomato" and Oryza sativa L. "rice" as an alternative to accumulation of petroleum-based plastics. Previously disinfected root were plated in Nfb semisolid medium where nitrogen-fixing bacteria were recognized by a whitish film on the surface and turn from green to blue. The genus Azospirillum was identified in Congo red agar medium, obtained 96 isolates of A. lipoferum and A. brasilense on tomato and rice. Batch fermentation was performed with broth Azotobacter modified feeding a saturated solution of malic acid every 12 hours and were stained with Sudan Black B. Strains were selected with the greatest number of PHAs granules (in tomato, 18 of A. lipoferum and 2 of A. brasilense; in rice, 10 of A. lipoferum and 10 of A. brasilense and quantified the biomass and PHAs. PHAs concentration reached 0.661 gL-1 in A. lipoferum KM(T-73 and 0.738 gL-1 in A. brasilense KM(T-19, both isolated from tomato. Strains of A. lipoferum and A. brasilense isolated from tomato reached a higher concentration of biomass and PHAs against the strains of rice.

  5. Caracterización de germoplasma de tomate (Lycopersicon esculentum, Mill con vistas a la implementación de un programa de fitomejoramiento participativo

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    A. Solís

    2001-01-01

    Full Text Available En las áreas de la Estación Territorial de Investigaciones Agropecuarias de Holguín (ETIAH, sobre un suelo Pardo sin carbonato, se realizó un estudio con 18 líneas de tomate (Lycopersicon esculentum, Mill obtenidas por el campesino Jorge Pupo1, con el objetivo de caracterizarlas y establecer una estrategia de mejora que permita alcanzar genotipos con características varietales adaptadas a las condiciones del territorio. La información disponible fue sometida a variados análisis de la estadística descriptiva y multivariada, ambos procederes adecuados para este tipo de trabajo, a fin de profundizar en la caracterización de la población. Los resultados indicaron una alta variabilidad y segregación genética en los materiales evaluados, por lo que se recomienda continuar el proceso de evaluación y selección hasta alcanzar la estabilidad varietal; no obstante, los de mayores perspectivas fueron la J10-87, J4-90, J4-92 y J6-93, que mostraron características agrícolas favorables y mayores potencialidades que las variedades más comúnmente cultivadas en el territorio.

  6. Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in Lambayeque

    Directory of Open Access Journals (Sweden)

    Cynthia Escobar

    2011-03-01

    Full Text Available The objective of this research was characterize and determine the effect of native strains of Azotobacter spp. on the vegetative growth of Lycopersicon esculentum Mill. "Tomato" as an alternative to the indiscriminate use of chemical fertilizers. Samples were taken from roots and rhizosphere soil of vegetables from which dilutions (10-4 in Ashbysucrose broth and incubated at 30 ° C to observe a yellow color, turbidity and surface film. The genus Azotobacter was identified in mineral agar without nitrogen and Ashby-benzoate, obtaining 96 strains with a yield of 7.10 to 57.99 mgL-1 indoleacetic acid, 0.13 to 1.64 mgL-1 of fixed nitrogen as ammonium and up to 1.61% efficiency in the solubilization of phosphate Bayovar rock. It obtained a cell suspension (10 8 of each of the four strains with the highest values and inoculated independently and in consortium, as well as a combination with 50% urea-100 % phosphate rock in the rhizosphere of Rio Grande tomato, in a completely randomized design. The entire native strains increased height, root volume, total dry matter, shoot and root, compared with absolute control.

  7. The impact of enhanced atmospheric carbon dioxide on yield, proximate composition, elemental concentration, fatty acid and vitamin C contents of tomato (Lycopersicon esculentum).

    Science.gov (United States)

    Khan, Ikhtiar; Azam, Andaleeb; Mahmood, Abid

    2013-01-01

    The global average temperature has witnessed a steady increase during the second half of the twentieth century and the trend is continuing. Carbon dioxide, a major green house gas is piling up in the atmosphere and besides causing global warming, is expected to alter the physico-chemical composition of plants. The objective of this work was to evaluate the hypothesis that increased CO(2) in the air is causing undesirable changes in the nutritional composition of tomato fruits. Two varieties of tomato (Lycopersicon esculentum) were grown in ambient (400 μmol mol(-1)) and elevated (1,000 μmol mol(-1)) concentration of CO(2) under controlled conditions. The fruits were harvested at premature and fully matured stages and analyzed for yield, proximate composition, elemental concentration, fatty acid, and vitamin C contents. The amount of carbohydrates increased significantly under the enhanced CO(2) conditions. The amount of crude protein and vitamin C, two important nutritional parameters, decreased substantially. Fatty acid content showed a mild decrease with a slight increase in crude fiber. Understandably, the effect of enhanced atmospheric CO(2) was more pronounced at the fully matured stage. Mineral contents of the fruit samples changed in an irregular fashion. Tomato fruit has been traditionally a source of vitamin C, under the experimental conditions, a negative impact of enhanced CO(2) on this source of vitamin C was observed. The nutritional quality of both varieties of tomato has altered under the CO(2) enriched atmosphere.

  8. Bioefficacy, residue dynamics and safety assessment of the combination fungicide trifloxystrobin 25% + tebuconazole 50%-75 WG in managing early blight of tomato (Lycopersicon esculentum Mill.).

    Science.gov (United States)

    Saha, Sujoy; Purath, Ahammed Shabeer Thekkum; Jadhav, Manjusha R; Loganathan, M; Banerjee, Kaushik; Rai, A B

    2014-01-01

    This paper reports the in vitro and in vivo bioefficacy of a combination fungicide trifloxystrobin (25%) + tebuconazole (50%) against early blight disease of tomato (Lycopersicon esculentum Mill.) caused by Alternaria solani and their corresponding pre-harvest intervals (PHI) with reference to the maximum residue limits (European Union). Bioefficacy of the test fungicide combination revealed that in vitro conditions manifested the best control (75.1%) at 350 mg kg(-1) against 76.2% control under field conditions. A sample preparation method based on ethyl acetate extraction and estimation by LC-MS multiple reaction monitoring (MRM) was validated in tomato fruits at 0.01 mg/kg and dissipation studies were conducted in field at single and double doses. The residues of both the compounds on all the sampling days were below the European Union maximum residue limits (EU-MRLs) and the maximum permissible intakes (MPIs) were calculated on the basis of prescribed acceptable daily intake (ADI). The combined bioefficacy and residue dynamics information will support label-claim of this fungicide combination for the management of early blight in tomato.

  9. Developmental changes in antioxidant metabolites, enzymes, and pigments in fruit exocarp of four tomato (Lycopersicon esculentum Mill.) genotypes: beta-carotene, high pigment-1, ripening inhibitor, and 'Rutgers'.

    Science.gov (United States)

    Torres, C A; Andrews, P K

    2006-01-01

    In surface cell layers of fleshy fruit, antioxidants must limit photooxidative reactions that generate reactive oxygen species (ROS) in high light. Our objective was to measure changes in the concentrations of antioxidant metabolites and pigments, and the activities of enzymes of the Mehler-peroxidase, ascorbate-glutathione cycle in fruit exocarp tissue under non-stress conditions of the following fruit-specific tomato (Lycopersicon esculentum Mill.=Solanum lycopersicum) mutants and their parent: (1) beta-carotene (B), (2) high pigment (hp-1), (3) ripening inhibitor (rin), and (4) the nearly isogenic wild-type 'Rutgers'. Developmental variables included days after anthesis (DAA) and fruit surface color. The highest total ascorbic acid (AsA) concentration was in the exocarp of immature green fruit of hp-1, being 32% higher than 'Rutgers'. The hp-1 mutant also had the highest chlorophyll and total carotenoid concentrations, comprised mostly of lycopene in red ripe fruit; whereas, beta-carotene comprised 90% of the carotenoids in B. Although enzyme activities varied within genotype, they generally increased with development, then decreased as fruit maturity was reached, being coupled with AsA and glutathione (GSH) concentrations. In all mutants, dark-green (DG) exocarp had more chlorophyll and protein, higher concentrations of reduced AsA and GSH, and usually lower enzyme activities than light-green (LG) exocarp taken from the same fruit.

  10. Study of radiation in fresh tomatoes (lycopersicon esculentum Mill) and in the levels of sauce lycopene; Estudo da radiacao ionizante em tomates in natura (lycopersicum esculentum Mill) e no teor de licopeno do molho

    Energy Technology Data Exchange (ETDEWEB)

    Fabbri, Adriana Diaz Toni

    2009-07-01

    The purpose of the present study was to determine the effects of ionizing radiation in tomatoes (Lycopersicon esculentum Mill) and at the same time to evaluate the influence of irradiation on the content of lycopene in a sauce made with these fruits irradiated. For this reason tomatoes were subjected to five treatments: control, T1 (0.25 kGy), T2 (0.5 kGy), T3 (1.0 kGy) and T4 (2.0 kGy) and evaluated at 3, 6, 9, 12, 15 and 20 days after irradiation, for the following analysis: maturity, texture, color, soluble solids, pH, acidity and total mass. Measurements were made of lycopene in fresh tomatoes and sauce by open column chromatography according to the ripening of them. The results showed that only the mass was not significantly different (p> 0.05). Treatments 1 and 2 proved to be effective in delaying the maturation and in the maintenance of firm texture (p <0.05). While T4 caused chemical reactions in the structure of tomato, compelling it to mature earlier because of pectin degradation. The completion of a sauce made from tomatoes irradiated to 0.25 kGy, didn't show a significant difference (p> 0.05) when compared to control. However, it didn't indicate lycopene degradation, as the doses of 1.0 and 2.0 kGy. Thus we can conclude that low doses are effective for maintaining pH, firmness, delayed senescence, mass and, in addition to not degrading the major bioactive compound of tomato, lycopene, suggest a higher bioavailability of this one, depending on the radiation application. (author)

  11. Caracterización de cepas nativas de Azotobacter spp. y su efecto en el desarrollo de Lycopersicon esculentum Mill. “tomate” en Lambayeque

    Directory of Open Access Journals (Sweden)

    Cynthia Escobar

    2011-01-01

    Full Text Available El objetivo de la presente investigación fue caracterizar y determinar el efecto de cepas nativas de Azotobacter spp. en el desarrollo vegetativo de Lycopersicon esculentum Mill. “tomate”, como una alternativa al uso indiscriminado de fertilizantes químicos. Se tomaron muestras de raíces y suelo rizosférico de hortalizas con las que se realizaron diluciones (10-4 en caldo Ashby-Sacarosa y se incubaron a 30 ºC hasta observar un color amarillo, turbidez y película superficial. El género Azotobacter se identificó en agar mineral sin nitrógeno y Ashby-Benzoato, obteniéndose 96 cepas con una producción de 7.10 a 57.99 mgL-1 de ácido indolacético, 0.13 a 1.64 mgL-1 de nitrógeno fijado como amonio y hasta 1.61 % de eficiencia en la solubilización de roca fosfórica de Bayóvar. Se obtuvo una suspensión celular (108 de cada una de las cuatro cepas con los mayores valores y se inocularon independientemente y en consorcio, así como una combinación con 50 % de urea-100 % de roca fosfórica, en la rizósfera de tomate cv. Río Grande, en un diseño experimental completamente aleatorio. Todas las cepas nativas incrementaron la altura, volumen radicular, materia seca total, parte aérea y radicular frente al testigo absoluto.

  12. Polihidroxialcanoatos de cepas de Azospirillum spp. aisladas de raíces de Lycopersicon esculentum Mill. “tomate” y Oryza sativa L. “arroz” en Lambayeque

    Directory of Open Access Journals (Sweden)

    Katty Baca

    2010-01-01

    Full Text Available En este trabajo se determinó la concentración de polihidroxialcanoatos (PH As de cepas de Azospirillum aisladas de raíces de Lycopersicon esculentum Mill. “tomate” y Oryza sativa L. “arroz”, como una alternativa ante la acumulación de plásticos derivados del petróleo. R aíces previamente desinfectadas se sembraron en medio Nfb se misólido, donde las bacterias fijadoras de nitrógeno se reconocieron por una película blanquecina bajo la superficie y el viraje del indicador al azul. El género Azospirillum se identificó en medio rojo de Congo, obteniéndose 96 cepas de A. lipoferum y A. brasilense en tomate y arroz. Se realizó una fermentación discontinua con caldo Azotobacter modificado, alimentando con una solución saturada de ácido málico cada 12 horas y se realizaron tinciones con Sudán Negro B. Se seleccionaron las cepas con mayor nú mero de gránulos de PHAs (en tomate , 18 de A. lipoferum y 2 de A. brasilense y en arroz, 10 de A. lipoferum y 10 de A. brasilense y se cuantificó la biomasa y PHAs. La concentración de PHAs alcanzó 0 . 661 gL - 1 en A. lipoferum KM(T - 73 y 0 . 738 gL - 1 en A. br asilense KM(T - 19. Las cepas de A. lipoferum y A. brasilense aisladas de tomate alcanzaron una mayor concentración de biomasa y PHAs frente a las cepas aisladas de arroz.

  13. β(1,3 GLUCANASAS DE LOS ESPACIOS INTERCELULARES DE HOJAS DE TOMATE LYCOPERSICON ESCULENTUM CERACIFORME DESPUES DE INFECCIÓN CON PHYTOPHTHORA INFESTANS.

    Directory of Open Access Journals (Sweden)

    Humberto M. Zamora

    2009-06-01

    Full Text Available Análisis en líquido de lavado intercelular de Lycopersicon esculentum ceraciforme L1568 perteneciente al germoplasma nacional con resistencia en campo a P. infestans mostró la presencia de cuatro   β (1,3 glucanasas después de infección con el patógeno. Las plantas se inocularon con el patógeno para inducir aumento en la concentración de las proteínas PR. Después de 15 días de inoculadas, se extrajo el líquido de lavado intercelular de los foliolos de la planta utilizando agua destilada. Las proteínas extraídas se liofilizaron, se  resuspendieron en buffer, se purificaron utilizando cromatografía de intercambio iónico y electroforesis preparativa,  esto permitió la  caracterización parcial de una β (1,3  glucanasa básica con peso molecular  de 36,8 kDa y PI  9,2 y tres ácidas de  35.4, 30.1 y 7.2 kDa  con puntos isoeléctricos de 3.8; 3.6 y 5.0 respectivamente. Además se determinaron las propiedades cinéticas de cada enzima, KM, Vmax, Eo y K3 encontrando que la proteína básica no ha sido reportada en la literatura hasta ahora.

  14. Exposure of Lycopersicon esculentum to microcystin-LR: effects in the leaf proteome and toxin translocation from water to leaves and fruits.

    Science.gov (United States)

    Gutiérrez-Praena, Daniel; Campos, Alexandre; Azevedo, Joana; Neves, Joana; Freitas, Marisa; Guzmán-Guillén, Remédios; Cameán, Ana María; Renaut, Jenny; Vasconcelos, Vitor

    2014-06-11

    Natural toxins such as those produced by freshwater cyanobacteria have been regarded as an emergent environmental threat. However, the impact of these water contaminants in agriculture is not yet fully understood. The aim of this work was to investigate microcystin-LR (MC-LR) toxicity in Lycopersicon esculentum and the toxin accumulation in this horticultural crop. Adult plants (2 month-old) grown in a greenhouse environment were exposed for 2 weeks to either pure MC-LR (100 μg/L) or Microcystis aeruginosa crude extracts containing 100 μg/L MC-LR. Chlorophyll fluorescence was measured, leaf proteome investigated with two-dimensional gel electrophoresis and Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF)/TOF, and toxin bioaccumulation assessed by liquid chromatography-mass spectrometry (LC-MS)/MS. Variations in several protein markers (ATP synthase subunits, Cytochrome b6-f complex iron-sulfur, oxygen-evolving enhancer proteins) highlight the decrease of the capacity of plants to synthesize ATP and to perform photosynthesis, whereas variations in other proteins (ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit and ribose-5-phosphate isomerase) suggest an increase of carbon fixation and decrease of carbohydrate metabolism reactions in plants exposed to pure MC-LR and cyanobacterial extracts, respectively. MC-LR was found in roots (1635.21 μg/kg fw), green tomatoes (5.15-5.41 μg/kg fw), mature tomatoes (10.52-10.83 μg/kg fw), and leaves (12,298.18 μg/kg fw). The results raise concerns relative to food safety and point to the necessity of monitoring the bioaccumulation of water toxins in agricultural systems affected by cyanotoxin contamination.

  15. Long-distance signals regulating stomatal conductance and leaf growth in tomato (Lycopersicon esculentum) plants subjected to partial root-zone drying.

    Science.gov (United States)

    Sobeih, Wagdy Y; Dodd, Ian C; Bacon, Mark A; Grierson, Donald; Davies, William J

    2004-11-01

    Tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) plants were grown with roots split between two soil columns. After plant establishment, water was applied daily to one (partial root-zone drying-PRD) or both (well-watered control-WW) columns. Water was withheld from the other column in the PRD treatment, to expose some roots to drying soil. Soil and plant water status were monitored daily and throughout diurnal courses. Over 8 d, there were no treatment differences in leaf water potential (psileaf) even though soil moisture content of the upper 6 cm (theta) of the dry column in the PRD treatment decreased by up to 70%. Stomatal conductance (gs) of PRD plants decreased (relative to WW plants) when of the dry column decreased by 45%. Such closure coincided with increased xylem sap pH and did not require increased xylem sap abscisic acid (ABA) concentration ([X-ABA]). Detached leaflet ethylene evolution of PRD plants increased when of the dry column decreased by 55%, concurrent with decreased leaf elongation. The physiological significance of enhanced ethylene evolution of PRD plants was examined using a transgenic tomato (ACO1AS) with low stress-induced ethylene production. In response to PRD, ACO1AS and wild-type plants showed similar xylem sap pH, [X-ABA] and gs, but ACO1AS plants showed neither enhanced ethylene evolution nor significant reductions in leaf elongation. Combined use of genetic technologies to reduce ethylene production and agronomic technologies to sustain water status (such as PRD) may sustain plant growth under conditions where yield would otherwise be significantly reduced.

  16. Identification and quantification of stilbenes in fruits of transgenic tomato plants (Lycopersicon esculentum Mill.) by reversed phase HPLC with photodiode array and mass spectrometry detection.

    Science.gov (United States)

    Nicoletti, Isabella; De Rossi, Antonella; Giovinazzo, Giovanna; Corradini, Danilo

    2007-05-02

    Reversed-phase high-performance liquid chromatography (RP-HPLC) with photodiode array (PDA) and mass spectrometry (MS) detection was employed to study the accumulation of stilbenes and other naturally occurring polyphenol intermediates of flavonoid pathway in tomato fruits of plants genetically modified to synthesize resveratrol. The transgenic tomato fruits were obtained by overexpression of a grapevine gene encoding the enzyme stilbene synthase in tomato plants (Lycopersicon esculentum Mill.). Stilbenes and flavonoids, either glycosylated or free, were simultaneosly identified by electrospray interface (ESI)-MS in negative ionization mode and were quantified by PDA detection at the wavelength corresponding to their maximum absorbance. The two detectors were coupled online with an HPLC system utilizing a narrow-bore C18 reversed-phase column, which was eluted by a multistep gradient of increasing concentration of acetonitrile in water containing 0.5% (v/v) formic acid. The results of these analysis revealed that the genetic modification of the tomato plants originated different levels of accumulation of four stilbenes (i.e., trans- and cis-piceid and trans- and cis-resveratrol) in their fruit depending on the stages of ripening. Either at immature or at mature stages of ripening the stilbenes were preferentially accumulated in the fruit peel as the glycosylated form. The highest amount of trans-piceid and trans-resveratrol were found in the peel of fruits harvested at mature stage of ripening. The variations in the levels of rutin, naringenin, and chlorogenic acid found in the samples extracted from the fruits of transgenic tomato plants, in comparison to that determined in the control lines, seemed to be related to the genetic transformation, whose effect on the flavonoid biosynthetic pathway needs to be elucidated by additional studies.

  17. Exposure of Lycopersicon Esculentum to Microcystin-LR: Effects in the Leaf Proteome and Toxin Translocation from Water to Leaves and Fruits

    Directory of Open Access Journals (Sweden)

    Daniel Gutiérrez-Praena

    2014-06-01

    Full Text Available Natural toxins such as those produced by freshwater cyanobacteria have been regarded as an emergent environmental threat. However, the impact of these water contaminants in agriculture is not yet fully understood. The aim of this work was to investigate microcystin-LR (MC-LR toxicity in Lycopersicon esculentum and the toxin accumulation in this horticultural crop. Adult plants (2 month-old grown in a greenhouse environment were exposed for 2 weeks to either pure MC-LR (100 μg/L or Microcystis aeruginosa crude extracts containing 100 μg/L MC-LR. Chlorophyll fluorescence was measured, leaf proteome investigated with two-dimensional gel electrophoresis and Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF/TOF, and toxin bioaccumulation assessed by liquid chromatography-mass spectrometry (LC-MS/MS. Variations in several protein markers (ATP synthase subunits, Cytochrome b6-f complex iron-sulfur, oxygen-evolving enhancer proteins highlight the decrease of the capacity of plants to synthesize ATP and to perform photosynthesis, whereas variations in other proteins (ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit and ribose-5-phosphate isomerase suggest an increase of carbon fixation and decrease of carbohydrate metabolism reactions in plants exposed to pure MC-LR and cyanobacterial extracts, respectively. MC-LR was found in roots (1635.21 μg/kg fw, green tomatoes (5.15–5.41 μg/kg fw, mature tomatoes (10.52–10.83 μg/kg fw, and leaves (12,298.18 μg/kg fw. The results raise concerns relative to food safety and point to the necessity of monitoring the bioaccumulation of water toxins in agricultural systems affected by cyanotoxin contamination.

  18. 5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes.

    Science.gov (United States)

    Rosati, Fabiana; Bardazzi, Irene; De Blasi, Paola; Simi, Lisa; Scarpi, Dina; Guarna, Antonio; Serio, Mario; Racchi, Milvia L; Danza, Giovanna

    2005-08-01

    The full-length cDNA (LeDET2) encoding a 257 amino acid protein homolog of Arabidopsis DET2 (AtDET2) was isolated in tomato (Lycopersicon esculentum). LeDET2 has 76% similarity with AtDET2 and structural characteristics conserved among plant and mammalian steroid 5alpha-reductases (5alphaRs). LeDET2 is ubiquitously expressed in tomato tissues with higher levels in leaf than in stem, root, seed and callus. When expressed in mammalian cells (COS-7), recombinant LeDET2 was active on substrates typical of mammalian 5alphaRs (progesterone, testosterone, androstenedione), but reduced at very low levels campestenone, the substrate described for AtDET2. Similar results were obtained with the expression in COS-7 of recombinant AtDET2 that showed 5alphaR activity for progesterone and not for campestenone. Recombinant LeDET2 was inhibited by several inhibitors of the human 5alphaRs and the application of an active inhibitor to tomato seedlings induced dwarfism and morphological changes similar to BR-deficient mutants. In tomato tissues, campestenone was 5alpha-reduced in leaf, stem and root homogenates, like progesterone and testosterone, while androstenedione was converted to testosterone, evidencing for the first time a 17beta-hydroxysteroid dehydrogenase activity in plants. Moreover, two separate 5alphaR activities with different kinetic characteristic and response to inhibitors were characterized in tomato tissues. The presence of two 5alphaR isoenzymes was demonstrated also in Arabidopsis using the det2-1 mutant, in which a residual 5alphaR activity for campestenone and progesterone was evidenced and characterized. Therefore, the existence of two isoenzymes of 5alphaR is probably characteristic of the whole plant kingdom highlighting the similarities between the animal and plant steroid biosynthetic pathways.

  19. Habilidad combinatoria para el carácter producción por planta y sus componentes primarios en un cruzamiento dialélico de siete líneas de tomate "Chonto" Lycopersicon esculentum, Mill

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    Vallejo Cabrera Franco Alirio

    1990-06-01

    Full Text Available Se evaluaron siete líneas progenitoras de tomate "Chonto" (Ángela Gigante, Licapal21, Raminho, Olho Roxo, 1258,1475 Y 1507 y 21 híbridos, sin incluir los recíprocos, mediante el método experimental 2 y modelo tipo 1 propuesto por Griffing (1956. En la expresión de los caracteres producción por planta y peso promedio de fruto se encontraron diferencias significativas tanto para la habilidad combinatoria general (acción génica aditiva como para la habilidad combinatoria específica (acción génica no aditiva, pero con predominio de ésta última. En la expresión del carácter número de frutos por planta se encontró diferencias para los dos tipos de habilidad combinatoria pero, con predominio de la acción génica aditiva. Los híbridos Licapal 21 x Raminho, Ángela Gigante x 1258 y Ángela Gigante x Raminho exhibieron los mayores efectos de habilidad combinatoria específica y los mayores valores promedios, para el carácter producción por planta.An analysis of combining ability of traits related with production per plant was carried out using a diallel crossing between different "Chonto" tomato cultivar, Lycopersicon esculentum Mill. (Seven parents and 21 F1 hybrids from all possible crossing in one direction, according to the methodology propose d by Griffing (1956, selecting experimental method 2, and model. Significant differences were found for the two types of combining ability (g.c.a. and s.c.a. on the expression of the characters production per plant, fruits per plant and fruit mean weight. A non-additive gene action was prevailing for the characters production per plant and fruit mean weight. Hybrids Licapal- 21 x Raminho, Angela Gigante x 1258 and Angela Gigante x Rarninho, exhibited high s.c.a. effects and heigh mean production per plant.

  20. Effects of exogenous epibrassinolide on photosynthetic characteristics in tomato (Lycopersicon esculentum Mill) seedlings under weak light stress.

    Science.gov (United States)

    Wang, Ming; Jiang, Weijie; Yu, Hongjun

    2010-03-24

    The effects of three concentrations (0.1, 0.01, 0.001 mg/kg) of exogenous 24-epibrassinolide on leaf photosynthesis, chlorophyll content, chlorophyll fluorescence, and parameters of light response curve in tomato seedlings under 150 micromol x m(-2) x s(-1) weak light stress were studied, with two tomato cultivars, 'Zhongza9', tolerant, and 'Zhongshu6', sensitive to weak light stress. The results showed that the net photosynthetic rate (Pn), maximal photochemical quantum efficiency of PSII (Fv/Fm), light saturation point (LSP), and dark respiration rate (Rd) decreased remarkably under weak light, but the chlorophyll content, especially chlorophyll b (chlb) content, increased obviously compared with normal light intensity control. However, exogenous 24-epibrassinolide alleviated the decrease of leaf Pn and Fv/Fm and induced the further increase of chlb content as well as the further decrease of Rd and chla/chlb under weak light stress, which indicated that exogenous 24-epibrassinolide could enhance plant tolerance to weak light and diminish damage from weak light. However, the optimum concentrations were different between the two cultivars; 0.1 mg/kg 24-epibrassinolide showed the best induction effects in 'Zhongshu6', and the best level for 'Zhongza9' was 0.01 mg/kg 24-epibrassinolide.

  1. CHANGES INDUCED BY HIGH TEMPERATURES IN PHOTOSYNTHESIS AND ANTIOXIDANT RESPONSE ON TWO GENOTYPES OF TOMATO (Lycopersicon esculentum

    Directory of Open Access Journals (Sweden)

    Daymi Camejo

    2002-01-01

    Full Text Available Dos cultivares de tomate con distinta susceptibilidad a las altas temperaturas (cv Amalia y el tipo silvestre Nagcarlang fueron expuestos a diferentes condiciones de estrés (45oC-2horas, 45oC-3horas y 25oC como control. El intercambio gaseoso y la fluorescencia de clorofila fueron determinados así como la respuesta antioxidante expresada por la actividad SOD y APX. Las variables fisiológicas y bioquímicas evaluadas fueron modificadas dependiendo del genotipo y el tiempo de exposición al estrés. El intercambio gaseoso fue reducido por la condición de estrés (45oC-3horas, mientras la fluorescencia de clorofila fue reducida desde las primeras horas de impuesto el estrés en el cv Amalia. Las variables evaluadas pueden ser útiles para describir genotipos tolerantes a las altas temperaturas.

  2. Effect of temperature on the occurrence of O/sub 2/ and CO/sub 2/ insensitive photosynthesis in field grown plants. [Phaseolus vulgaris; Capsicum annum; Lycopersicon esculentum, Scrophularia desertorum; Cardaria draba, Populus fremontii

    Energy Technology Data Exchange (ETDEWEB)

    Sage, R.F.; Sharkey, T.D.

    1987-07-01

    The sensitivity of photosynthesis to O/sub 2/ and CO/sub 2/ was measured in leaves from field grown plants of six species (Phaseolus vulgaris, Capsicum annuum, Lycopersicon esculentum, Scrophularia desertorum, Cardaria draba, and Populus fremontii) from 5/sup 0/C to 35/sup 0/C using gas-exchange techniques. In all species but Phaseolus, photosynthesis was insensitive to O/sub 2/ in normal air below a species dependent temperature. CO/sub 2/ insensitivity occurred under the same conditions that resulted in O/sub 2/ insensitivity. A complete loss of O/sub 2/ sensitivity occurred up to 22/sup 0/C in Lycopersicon but only up to 6/sup 0/C in Scrophularia. In Lycopersicon and Populus, O/sub 2/ and CO/sub 2/ insensitivity occurred under conditions regularly encountered during the cooler portions of the day. Because O/sub 2/ insensitivity is an indicator of feedback limited photosynthesis, these results indicate that feedback limitations can play a role in determining the diurnal carbon gain in the field. At higher partial pressures of CO/sub 2/ the temperature at which O/sub 2/ insensitivity occurred was higher, indicating that feedback limitations in the field will become more important as the CO/sub 2/ concentration in the atmosphere increases.

  3. Effect of Seed Inoculation with Pseudomonas fluorescence and Glomus esculentum on Quantitative and Qualitative Yield ofTwo Forage Corn Cultivars

    Directory of Open Access Journals (Sweden)

    M. Pourebrahimi

    2014-02-01

    Full Text Available In order to investigate the effect of Pseudomonas fluorescence strain 93 bacteria and Glomus esculentum fungus on quantitative and qualitative yield of two forage corn cultivars, an RCBD based factorial field experiment with three replications was conducted at the Agricultural Research Station of the University of Guilan in 2008. Treatments were considered as phosphorus chemical fertilizer, seed inoculation with Glomus esculentum mycorrhiza fungus, seed inoculation with Pseudomonas fluorescence strain 93 bacteria, seed inoculation with Pseudomonas fluorescence strain 93 bacteria and Glomus esculentum mycorrhiza fungus, and two corn cultivars (SC704 and SC647. No application of chemical fertilizer and microorganisms was considered as control. Results of ANOVA showed that plant height and leaf area, plant SPAD value, and plant biological yield were affected by cultivar × fertilizer interaction, significantly. The highest plant height (249.30 cm, SPAD value (52.30, and biological yield (251.51 g/plant were related to cultivar SC704 which treated with chemical phosphorous fertilizer or inoculated with bacteria-fungus combination. The maximum plant leaf area was achieved in chemical phosphorous fertilizer followed by application of both bacteria and fungus treatments. Inoculation with both bacteria and fungus also resulted to highest dry matter digestibility (77.37 % and crude protein (12.61 %. However, the highest (54.41 % and lowest (34.38 % crude fiber was related to control and bacteria-fungus combination treatments, respectively. Among two cultivars used in the experiment, SC704 was superior according to examine crop traits. Therefore, it could be expected that inoculating the seeds of SC704 corn cultivar with Pseudomonas fluorescence- Glomus esculentum combination resulted to reduce the use of phosphorus fertilizers and increase in the quality and quantity of forage corn.

  4. Callus formation and organogenesis of tomato (Lycopersicon ...

    African Journals Online (AJOL)

    USER

    2010-07-12

    Jul 12, 2010 ... In vitro culture response was assessed in tomato (Lycopersicon esculentum Mill. c. v. Omdurman) for ... successful application of plant tissue culture presupposes the establishment of ..... Kino (Pterocarpus marsupium Roxb.) ...

  5. Ribonuclease activity of buckwheat plant (Fagopyrum esculentum cultivars with different sensitivities to buckwheat burn virus

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    Y. R. Sindarovska

    2014-06-01

    Full Text Available Ribonucleases (RNases are present in base-level amounts in intact plants, but this level is able to increase greatly under stress conditions. The possible cause for such an increase is protection against plant RNA-virus attack. Buckwheat burn virus (BBV is a highly virulent pathogen that belongs to Rhabdoviridae family. In our study, we have analyzed the correlation between RNase activity and resistance of different buckwheat cultivars to BBV infection. Two cultivars, Kara-Dag and Roksolana, with different sensitivities to BBV have been used. Kara-Dag is a cultivar with medium sensitivity to virus and Roksolana is a tolerant cultivar. It has been shown that the base level of RNase activity in Roksolana cultivar was in most cases higher than the corresponding parameter in Kara-Dag cultivar. Both infected and uninfected plants of Roksolana cultivar demonstrated high RNase activity during two weeks. Whereas infected plants of Kara-Dag cultivar demonstrated unstable levels of RNase activity. Significant decline in RNase activity was detected on the 7th day post infection with subsequent gradual increase in RNase activity. Decline of the RNase activity during the first week could promote the virus replication and therefore more successful infection of upper leaves of plants. Unstable levels of RNase activity in infected buckwheat plants may be explained by insufficiency of virus-resistant mechanisms that determines the medium sensitivity of the cultivar to BBV. Thus, plants of buckwheat cultivar having less sensitivity to virus, displayed in general higher RNase activity.

  6. Ribonuclease activity of buckwheat plant (Fagopyrum esculentum) cultivars with different sensitivities to buckwheat burn virus.

    Science.gov (United States)

    Sindarovska, Y R; Guzyk, O I; Yuzvenko, L V; Demchenko, O A; Didenko, L F; Grynevych, O I; Spivak, M Ya

    2014-01-01

    Ribonucleases (RNases) are present in base-level amounts in intact plants, but this level is able to increase greatly under stress conditions. The possible cause for such an increase is protection against plant RNA-virus attack. Buckwheat burn virus (BBV) is a highly virulent pathogen that belongs to Rhabdoviridae family. In our study, we have analyzed the correlation between RNase activity and resistance of different buckwheat cultivars to BBV infection. Two cultivars, Kara-Dag and Roksolana, with different sensitivities to BBV have been used. Kara-Dag is a cultivar with medium sensitivity to virus and Roksolana is a tolerant cultivar. It has been shown that the base level of RNase activity in Roksolana cultivar was in most cases higher than the corresponding parameter in Kara-Dag cultivar. Both infected and uninfected plants of Roksolana cultivar demonstrated high RNase activity during two weeks. Whereas infected plants of Kara-Dag cultivar demonstrated unstable levels of RNase activity. Significant decline in RNase activity was detected on the 7th day post infection with subsequent gradual increase in RNase activity. Decline of the RNase activity during the first week could promote the virus replication and therefore more successful infection of upper leaves of plants. Unstable levels of RNase activity in infected buckwheat plants may be explained by insufficiency of virus-resistant mechanisms that determines the medium sensitivity of the cultivar to BBV. Thus, plants of buckwheat cultivar having less sensitivity to virus, displayed in general higher RNase activity.

  7. Evaluation of the use of radiation in microbiological control in tomato (Lycopersicon esculentum mill.) sold in the CEASA, Pernambuco

    Energy Technology Data Exchange (ETDEWEB)

    Vicalvi, Maria Claudia V.; Solidonio, Evelyne G.; Silva, Marcio de Albuquerque; Costa Junior, Carlos Eduardo de Oliveira; Alcantara, Moacir Paulo [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Pos-graduation program in Energetics and Nuclear Tecnologies; Miranda, Rita de Cassia M. de [Universidade Federal de Sergipe (UFS), Aracaju, SE (Brazil). Lab. of Chromatographic Analysis and Flavor; Sena, Kesia Xisto F.R. de [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. of Antibiotics; Colaco, Waldeciro, E-mail: wcolaco@ufpe.com.b [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. of Nuclear Energy

    2011-07-01

    Tomato is one of the most consumed fruits in the world and also one of the agricultural products with most losses due to its high perishability. Nevertheless, there are only a few studies on the post-harvest life of tomatoes and therefore are still many undefined parameters related to the treatment and shelf life of different cultivars of that fruit. Its contamination occurs from the pre-to post-harvest crop. The diseases caused by food borne pathogenic microorganisms are a serious public health problem. The bacteria of the Enterobacteriaceae are responsible for large outbreaks of gastroenteritis in humans that may occur due to bacteria per se or by toxins produced by them. Thus, the development of technologies that make the food safe for human consumption has been growing in recent years. Preservation methods employ physical or chemical processes. Among such methods, irradiation is a preventive treatment for food safety. Irradiation can reduce post-harvest losses by killing insect pests in fruits, grains, spices, or by reducing food spoilage organisms by inhibiting the sprouting of plants and delaying the ripening of fruits. The objective of this research was to evaluate the potential of using different doses of gamma radiation to treat tomatoes sold in CEASA-PE and to perform the overall count of microorganisms in control and irradiated samples. The study used two lots containing 60 tomatoes such that 15 of them were used as the control group and 45 were irradiated with one of 3 different doses (1.0 kGy, 1.5 kGy and 2.0 kGy). For microbiological analysis, the tomatoes were sliced, and weighed (after removal of peel) in order to obtain samples weighing 25g. Each sample was added to an Erlenmeyer containing sterile water by agitation for 15 minutes. Aliquots of the washings were sown for exhaustion in sheep blood agar. After growth, colony counting was performed. The results revealed contamination in all tomato groups analyzed with the control groups exhibiting

  8. RESPUESTA DIFERENCIAL A LA SALINIDAD DE GENOTIPOS DE TOMATE (Lycopersicon esculentum Mill. EN PRIMERAS ETAPAS FENOLÓGICAS

    Directory of Open Access Journals (Sweden)

    Francisco Higinio Ruiz Espinoza

    2014-09-01

    Full Text Available La salinidad afecta tanto el crecimiento vegetativo como el desarrollo reproductivo de las plantas: puede reducir el número de flores, incrementar la esterilidad y alterar la duración de la floración y la maduración. En México, el tomate es una de las especies hortícolas de mayor importancia económica y social, en virtud del valor de su producción y por la demanda de mano de obra que genera, por lo que el objetivo del presente trabajo fue evaluar el efecto de la salinidad en variables morfológicas, en dos etapas fenológicas (germinación y crecimiento vegetativo inicial, de ocho genotipos de tomate, “Tropic, Feroz, Ace, Super Río Grande, Yaqui, Missouri, Vita y Floradade”, los cuales fueron expuestos a estrés salino mediante la adición de NaCl en concentraciones de 0, 50, 100, 150 y 200 mM, en un diseño experimental completamente al azar con cuatro repeticiones. En la etapa de germinación se midieron las variables morfométricas: porcentaje de germinación, peso fresco y seco de parte aérea, peso fresco de raíces. En la etapa de crecimiento vegetativo inicial se midieron: longitud de tallo, peso fresco de tallo, numero de hojas y área foliar. Los resultados de la etapa de germinación muestran que conforme se incrementó la concentración salina, el porcentaje de germinación disminuyó, siendo “Tropic y Yaqui” los que mostraron valores superiores. Por su parte, “Tropic y Missouri” mostraron el mayor peso fresco y seco de parte aérea, mientras que los genotipos con menor tolerancia en esta etapa fueron “Feroz, Vita y Ace”. En la etapa de crecimiento vegetativo inicial se determinó que los cultivares que presentaron mejor respuesta o mayor tolerancia al estrés salino fueron “Missouri, Tropic, Feroz y Vita”, mientras que los genotipos con menor tolerancia a la salinidad en esta etapa fueron “Floradade y Ace”. Se concluye que en ambas etapas fenológicas existe un diferencial potencial entre los

  9. Metabolomic analysis and differential expression of anthocyanin biosynthetic genes in white- and red-flowered buckwheat cultivars (Fagopyrum esculentum).

    Science.gov (United States)

    Kim, Yeon Bok; Park, Soo-Yun; Thwe, Aye Aye; Seo, Jeong Min; Suzuki, Tastsuro; Kim, Sun-Ju; Kim, Jae Kwang; Park, Sang Un

    2013-11-01

    Red-flowered buckwheat ( Fagopyrum esculentum ) is used in the production of tea, juice, and alcohols after the detoxification of fagopyrin. In order to investigate the metabolomics and regulatory of anthocyanin production in red-flowered (Gan-Chao) and white-flowered (Tanno) buckwheat cultivars, quantitative real-time RT-PCR (qRT-PCR), gas chromatography time-of-flight mass spectrometry (GC-TOFMS), and high performance liquid chromatography (HPLC) were conducted. The transcriptions of FePAL, FeC4H, Fe4CL1, FeF3H, FeANS, and FeDFR increased gradually from flowering stage 1 and reached their highest peaks at flowering stage 3 in Gan-Chao flower. In total 44 metabolites, 18 amino acids, 15 organic acids, 7 sugars, 3 sugar alcohols, and 1 amine were detected in Gan-Chao flowers. Two anthocyanins, cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside, were identified in Gan-Chao cultivar. The first component of the partial least-squares to latent structures-discriminate analysis (PLS-DA) indicated that high amounts of phenolic, shikimic, and pyruvic acids were present in Gan-Chao. We suggest that transcriptions of genes involved in anthocyanin biosynthesis, anthocyanin contents, and metabolites have correlation in the red-flowered buckwheat Gan-Chao flowers. Our results may be helpful to understand anthocyanin biosynthesis in red-flowered buckwheat.

  10. Effects of super absorbent with different application methods on soil moisture, soil salinity and Lycopersicon esculentum growth%保水剂施用方式对土壤水盐及番茄生长的影响

    Institute of Scientific and Technical Information of China (English)

    耿桂俊; 白岗栓; 杜社妮; 于健; 李曼

    2011-01-01

    In order to determine the effects of BJ2101-L super absorbent with various application methods, field experiments were conducted to investigate their effects on soil moisture, soil salinity and Lycopersicon esculentum growth in Hetao irrigation district, Inner Mongolia.In this paper, BJ2101-L super absorbent was applied with four treatments of furrowing, mixing, holing and broadcasting.The results showed that furrowing, holing, mixing significantly increased soil moisture in 0 -80 cm soil layer,particularly there were significant effects during Lycopersicon esculentum fruit-setting stage, and soil moisture in furrowing, mixing and holing treatments increased by 12.66% , 9.40% and 7.50% in 0 -80 cm soil layer compared to the control respectively.BJ2101-L super absorbent inhibited the accumulation of soil salinity and could improve the survival rate of Lycopersicon esculentum seedlings.The yields in furrowing, mixing, holing and broadcasting treatments increased by 37.70% , 30.93% , 26.35% and 12.87% compared to the control, and water use efficiency increased by 56.58% , 44.98%, 32.58%and 24.05% against the control respectively.Optimum application method of BJ2101-L super absorbent should be furrowing application in Hetao irrigation district in Lycopersicon esculentum production.%为探讨保水剂不同施用方式的应用效果,在河套灌区,以不施为对照,开展撒施、穴施、沟施和混施BJ2101-L保水剂对土壤水分、盐分和番茄生长影响的田间试验.结果表明:1)沟施、穴施和混施均显著提高0~80 cm土层土壤水分,特别在番茄坐果期较对照提高了12.66%、9.40%和7.50%;2)保水剂可有效抑制土壤盐分的积累,提高番茄幼苗成活率,沟施、穴施、混施和撒施番茄产量较对照提高了37.70%、30.93%、26.35%和12.87%,水分利用效率提高了56.58%、44.98%、32.58%和24.05%.因此,河套灌区番茄生产中保水剂应以沟施为主.

  11. Capítulo VII: variabilidad genética y ambiental entre líneas de tomate "chonto", Lycopersicon esculentum Mill, sin utilizar pruebas de progenie

    Directory of Open Access Journals (Sweden)

    Vallejo Cabrera Franco Alirio

    1994-12-01

    Full Text Available

    This paper reports the results of a study on genetic and environmental variability between 43 lines of "Chonto" tomato, Lycopersicon esculentum Mill, without using progeny offspring test. The experimental material were planted under field conditions using three randomized blocks. Data on individual plants were recorded for yield/plant, number of fruit/plant, average fruit weight, number of inflorescences/plant, number of fruits/inflorescence, number of locules/fruit, average locule weight, fruit shape index, pericarp thickness, days to flowering, harvest lenght, plant heigh, first inflorescence heigh, internode lenght, reaction to the presence of Scrobipalpula absoluta and Xanthomonas vesicatoria. The quantitative characters were subjeted to variance analysis in orden to evaluate the genetic and environmental variation. Heritability in the wide sense, genetic variability coeficient and the expected progress by selection were estimated. Fenotipic, environmental and genetic correlations among some evaluated characters were also carried out. Important  genotipic differences, at 1% of probability, for the evaluated characters were found. Generally, in the most of the evaluated characters the largest contribution of the total variation correspond to the genetic variation. The contribution of the environmental variation was very low.

    Con el fin de analizar la variabilidad genética y ambiental, de algunos caracteres agronómicos importantes en tomate "chonto" se evaluaron 43 líneas, en condiciones de campo utilizando un diseño experimental de bloques al azar con tres repeticiones. Los datos experimentales se obtuvieron a partir de tres plantas individuales por repetición y en plena competencia. Se evaluaron los siguientes caracteres cuantitativos: producción por planta, número de frutos por planta, peso promedio de frutos, número de inflorescencias por planta, número de frutos por inflorescencias, número de lóculos por fruto, peso

  12. 番茄Metacaspase基因(LeM)的克隆及其在大肠杆菌Rosetta中的高效表达%Cloning of Lycopersicon esculentum Metacaspase Gene (LeM) and Its Effective Expression in Escherichia coli Rosetta

    Institute of Scientific and Technical Information of China (English)

    马秋敏; 张雅丽; 刘金娜; 车英慧; 谢寒丁; 罗云波; 曲桂芹

    2011-01-01

    为获得可溶性的番茄Metacaspase蛋白,后续研究Metacaspase蛋白的酶学特性以及功能,利用RT-PCR方法克隆了番茄(Lycopersicon esculentum)中Metacaspase(LeM)全长cDNA,并成功地构建了LeM基因的原核表达载体重组质粒pET28a-LeM,转入大肠杆菌(Escherichia coli)BL21(DE3)PlysS和Rosetta中进行诱导表达,并对诱导表达条件进行了优化.SDS-PAGE结果显示,在37℃条件下,1 mmol/L IPTG诱导表达量最高,在BL21(DE3)PlysS菌株中重组蛋白主要以包涵体形式存在,而相同条件下在Rosetta菌株中多为可溶性表达.同时酶活测定显示,Rosetta上清中LeM的活性为33 RFU/min,显著高于BL21(DE3)PlysS 7RFU/min.本实验表明,不同菌株对Metacaspase的可溶性表达有很大影响.%To obtain the fusion protein of Lyco persicon esculentum Metacaspase (LeM) and study the characteristics and function of it, we cloned the open reading frame of LeM in Lycopersicon esculentum by RT-PCR, and successfully constructed the prokaryotic expression vector pET28a-LeM for LeM expression.The recombinant plasmid pET28a-LeM was transformed into Escherichia coli BL21 (DE3)PlysS and Rosette for expression, induced by IPTG, and the conditions of the expression was optimized.SDS-PAGE revealed that the best expression was induced by 1 mmol/L IPTG at 37℃ and the recombinant protein expressed mainly as inclusion body in E.coli BL21(DE3)PlysS while as fusion protein in E.coli Rosetta at the same condition.Enzyme activity assay showed that the activity of LeM in E.coli Rosetta was 33 RFU/min, which was much higher than 7 RFU/min of E.coli BL21 (DE3)PlysS.We demonstrate that the strain has a great influence on the expression of LeM fusion protein.

  13. Study on Separation Force between Tomato(Lycopersicon esculentum)Fruit and Stalk%加工番茄果茎分离力研究

    Institute of Scientific and Technical Information of China (English)

    张进; 毕新胜; 肖彬彬; 黄国伟

    2012-01-01

    [Objective] The aim was to study separation force between tomato fruit and stalk, to provide a theoretical basis for realizing mechanized harvest of tomato in Xinjiang and designing its separation device. [ Method] The tomato of different sizes, types, maturity, which were now largely cultivated in Xinjiang, were selected to measured their separation force between fruit and stalk, and then the experimental data was analyzed using SPSS data processing system. [ Result ] The results showed that the separation force between the fruit and stalk was different among different tomato varieties, but the gap was not large, and the separation force between tomato fruit and stalk was 5 - 30 N. The separation force between fruit and stalk of tomato was became small with its increasing of maturity and fruit length, while which became big with fruit weight. The nonlinear relationship between separating force between fruit and stalk of tomato and its shape(X1 stands for tomato length,X2 stands for tomato diameter,X3 stands for tomato fruit weight) under red ripe, green ripe and yellow ripe were Y= -38. 724 173 1 - 0. 275 597 259 20X1 + 2.819 463 816 8Z, - 0.032 358 548 87X22 +0.001 019 364 693 3X32 ,F = 2. 046 9,P=0. 0193; Y=-6.682 740 10+0.455 089 173 7X1+ 0. 134 872 969 06X2 +0.151 548 034 07X3 - 0.005 066 287 946X1X2 - 0. 004 933 150 310X1X3 +0.004 653 746 236X2X3 ,F =3. 3805,P = 0. 0042;Y= 105.928 032 8-8. 830 147 147X1 +7. 367 868 154X2 - 0. 466 247 543 0X3 +0. 072 647 951 54X12 - 0. 071 425 628 00X22 + 0. 003 594 294 685X32,F = 2. 297 6 ,P = 0. 011 7, respectively. [ Conclusion] The study provides a theoretical basis on tomato harvesting machinery research.%[目的]研究加工番茄(Lycopersicon esculentum)果茎分离力,为实现新疆番茄机械化收获和设计番茄分离装置提供理论基础.[方法]以目前新疆大规模种植的加工番茄为研究对象,采集不同大小、种类、成熟度的加工番茄,测量果实与茎秆之间的分离力,运

  14. A reliable begomovirus inoculation method for screening Lycopersicon esculentum lines Um método de inoculação de begomovírus confiável para a seleção de linhagens de Lycopersicon esculentim

    Directory of Open Access Journals (Sweden)

    Alice K Inoue-Nagata

    2007-09-01

    Full Text Available Due to failures of infection after mechanical inoculation of begomovirus on tomato (Lycopersicon esculentum Mill. plants, an efficient and reliable begomovirus inoculation method was developed using the whitefly vector (Bemisia tabaci. Virus acquisition was carried out using a 'Tube-cage', made of a polypropylene tube, containing one to two day-old whiteflies and a tomato plant apex infected with a begomovirus isolate. After 48 h of acquisition access period, inoculation was done using a 'Ring-cage', containing viruliferous whiteflies, attached to the abaxial side of the leaf of young tomato plants. 48 hours after acquisition, the whiteflies were eliminated and the inoculated plants were incubated for 28 days under greenhouse conditions. Three viruliferous whiteflies per plant were enough to cause infection in susceptible tomato cv. Viradoro. This procedure was applied to test the resistance of tomato line 486-1 (resistant line in comparison with 'Viradoro' (susceptible cultivar. Dot blot hybridization confirmed the susceptibility of 'Viradoro' and the resistance of the line 486-1, showing the efficiency of this method for screening plants for disease resistance. This method is also a useful tool in detecting the presence of virus in inoculated (lower leaves and in non-inoculated (upper leaves in a given plant. Using this procedure, it was observed that the resistance of the line 486-1 probably is expressed in the inoculated leaf without virus translocation to the upper leaves.Devido às falhas encontradas no método de inoculação de begomovírus em plantas de tomateiro, um método eficiente e confiável de inoculação de begomovírus foi desenvolvido utilizando como vetor a mosca-branca (Bemisia tabaci. A aquisição viral foi realizada em uma armadilha ('Tube-cage' feita a partir de um tubo de polipropileno contendo moscas-brancas de um ou dois dias de idade e o ápice de uma planta de tomate infectada com um begomovírus. Após 48 horas de

  15. Analysis of low-temperature tolerance of a tomato (Lycopersicon esculentum) cybrid with chloroplasts from a more chilling-tolerant L-hirsutum accession

    NARCIS (Netherlands)

    Venema, JH; Eekhof, M; van Hasselt, PR

    2000-01-01

    Growth and photosynthesis of an alloplasmic tomato (cybrid), i.e. line AH47, containing the nuclear genome of the chilling-sensitive cytoplasmic albino mutant of L. esculentum Mill. 'Large Red Cherry' (LRC) and the plastome of a more chilling-tolerant high-altitude accession of the related wild spec

  16. 番茄果实中成熟衰老相关small RNAs的最新研究进展%Latest Research Progress of Small RNAs Related to Tomato (Lycopersicon esculentum) Fruit Ripening and Senescence

    Institute of Scientific and Technical Information of China (English)

    左进华; 王清; 鞠正; 曹东艳; 朱本忠; 罗云波; 高丽朴

    2016-01-01

    番茄(Lycopersicon esculentum)是研究果实成熟衰老的模式材料,其成熟是一个多基因调控的高度协调的遗传调控过程,伴随着色泽、质地、风味、香气和其他代谢等许多显著的生理生化变化.近年来,small RNAs作为一种新型的转录后调控因子受到越来越多的关注,几乎参与了动植物中所有的生理和生化过程.在植物中参与生长发育、果实成熟、生物和非生物胁迫反应等过程.本文系统地综述了番茄果实中small RNAs的分离鉴定现状,对番茄果实中与乙烯信号途径、颜色、风味、软化等代谢途径相关的small RNAs进行了系统总结,并展望了未来small RNAs在果实成熟过程中的相关研究方向.

  17. Anti-phytopathogen potential of endophytic actinobacteria isolated from tomato plants (Lycopersicon esculentum) in southern Brazil, and characterization of Streptomyces sp. R18(6), a potential biocontrol agent.

    Science.gov (United States)

    de Oliveira, Margaroni Fialho; da Silva, Mariana Germano; Van Der Sand, Sueli T

    2010-09-01

    Tomato plants (Lycopersicon esculentum) are highly susceptible to phytopathogen attack. The resulting intensive application of pesticides on tomato crops can affect the environment and health of humans and animals. The objective of this study was to select potential biocontrol agents among actinobacteria from tomato plants, in a search for alternative phytopathogen control. We evaluated 70 endophytic actinobacteria isolated from tomato plants in southern Brazil, testing their antimicrobial activity, siderophore production, indoleacetic acid production, and phosphate solubility. The actinomycete isolate with the highest antimicrobial potential was selected using the agar-well diffusion method, in order to optimize conditions for the production of compounds with antimicrobial activity. For this study, six growth media (starch casein-SC, ISP2, Bennett's, Sahin, Czapek-Dox, and TSB), three temperatures (25 degrees C, 30 degrees C, and 35 degrees C) and different pH were tested. Of the actinobacteria tested, 88.6% showed antimicrobial activity against at least one phytopathogen, 72.1% showed a positive reaction for indoleacetic acid production, 86.8% produced siderophores and 16.2% showed a positive reaction for phosphate solubility. Isolate R18(6) was selected due to its antagonistic activity against all phytopathogenic microorganisms tested in this study. The best conditions for production were observed in the SC medium, at 30 degrees C and pH 7.0. The isolate R18(6) showed close biochemical and genetic similarity to Streptomyces pluricolorescens.

  18. LeMAPK1, LeMAPK2, and LeMAPK3 are associated with nitric oxide-induced defense response against Botrytis cinerea in the Lycopersicon esculentum fruit.

    Science.gov (United States)

    Zheng, Yanyan; Hong, Hui; Chen, Lin; Li, Jingyuan; Sheng, Jiping; Shen, Lin

    2014-02-12

    Nitric oxide (NO) and mitogen-activated protein kinases (MAPKs) are signal molecules involved in the disease resistance of plants. To investigate the role of tomato MAPKs in the NO-mediated defense response, mature green tomatoes (Lycopersicon esculentum Mill. cv. Qian-xi) were treated with a MAPKs inhibitor (1,4-diamino-2,3-dicyano-1,4-bis(o-amino-phenylmercapto) butadiene (U0126)), NO donor sodium nitroprusside (SNP), and SNP plus U0126. Treatment with U0126 increased the incidence of disease and size of lesion areas in the tomato fruits after being inoculated with Botrytis cinerea. NO enhanced the resistance of the tomato fruits against Botrytis cinerea invasion and the activities of nitric oxide synthase, Chitinase, β-1,3-glucanase, polyphenol oxidase, and phenylalanine ammonia-lyase. However, the effects of NO on disease resistance were weakened by the MAPKs inhibitor. Meanwhile, the relative expression of LeMAPK1, LeMAPK2, and LeMAPK3 in the (SNP + U0126)-treated fruits was lower than that in the SNP-treated fruits. The results suggest that LeMAPK1/2/3 are involved in NO-induced disease resistance of tomato fruits against Botrytis cinerea.

  19. Regeneração de plantas híbridas entre Lycopersicon esculentum e L. peruvianum a partir de calos com dois anos de cultura in vitro Regeneration of Lycopersicon esculentum X L. peruvianum hybrid plants from two year old callus culture

    Directory of Open Access Journals (Sweden)

    Walter José Siqueira

    1988-01-01

    Full Text Available Calos obtidos da cultura in vitro de embrião imaturo do cruzamento interespecífico L. esculentum x L. peruvianum, praticamente perderam a capacidade morfogenética, após dois anos de subcultura. Na tentativa de recuperação do processo de organogênese desses calos, realizaram-se dois experimentos, utilizando-se os fitorreguladores ácido indolacético (IAA e 6-benziladenina (6-BA, cujas concentrações foram combinadas em dialélicos de 5 x 5 e 3 x 3. A composição de sais minerais e vitaminas baseou-se no meio de Murashige e Skoog, adicionando-se sacarose a 3% e ágar a 0,8%, e ajustando-se o pH final dos meios de cultura para 5,5. As condições para o dialélico 5 x 5 foram fotoperíodo de 16 horas de luz a 600 lux e temperatura de 25 ± 3°C. No dialélico 3 x 3, os tratamentos foram mantidos em câmara de crescimento a 2.000 lux, sob a mesma variação de temperatura e fotoperíodo. Em cada frasco, inoculou-se um calo com cerca de 1 cm³, totalizando quinze repetições. Avaliaram-se o desenvolvimento de calos, atribuindo-se uma escala de notas de 1 a 5, e a presença de plantas (organogênese após 30 dias de cultura. Observou-se o número total de plantas por tratamento, bem como o desenvolvimento das plantas em centímetro. No dialélico 5 x 5, a organogênese foi apenas incipiente em três tratamentos, porém as melhores combinações para o desenvolvimento dos calos foram de 0,5, 2,5 e 5,0µM de IAA com 2,5µM de 6-BA. No dialélico 3 x 3, houve a indução de plantas em sete tratamentos, sendo mais eficientes 25 e 50µM de 6-BA, sem auxina. O tratamento de 0,5 e 10,0µM de IAA e 6-BA, respectivamente, permitiu simultaneamente o crescimento de calos e a regeneração de plantas. Nota-se a influência das condições ambientais de manutenção das culturas, principalmente da intensidade de luz.Callus from Lycopersicon esculentum x L. peruvianum interespecific hybrids cultured in vitro lost their morphogenetic abilities after two

  20. A bench-scale, cost effective and simple method to elicit Lycopersicon esculentum cv. PKM1 (tomato) plants against Cucumber mosaic virus attack using ozone-mediated inactivated Cucumber mosaic virus inoculum.

    Science.gov (United States)

    Sudhakar, N; Nagendra-Prasad, D; Mohan, N; Murugesan, K

    2007-12-01

    Studies were undertaken to evaluate ozone for inactivation of Cucumber mosaic virus present in the inoculum and to stimulate Lycopersicon esculentum cv. PKM1 (tomato) plants against Cucumber mosaic virus infection by using the inactivated Cucumber mosaic virus inoculum. Application of a T(4) (0.4mg/l) concentration of ozone to the inoculum containing Cucumber mosaic virus resulted in complete inactivation of the virus. The inactivated viral inoculum was mixed with a penetrator (delivery agent), referred to as T(4) preparation, and it was evaluated for the development of systemic acquired resistance in the tomato plants. Application of a T(4) preparation 5 days before inoculation with the Cucumber mosaic virus protected tomato plants from the effects of Cucumber mosaic virus. Among the components of the inactivated virus tested, coat protein subunits and aggregates were responsible for the acquired resistance in tomato plants. In field trials, the results of enzyme-linked immunosorbent assay revealed that, Cucumber mosaic virus accumulation was significantly less for all the test plants (16%) sprayed with the T(4) preparation than untreated control plants (89.5%) at 28 days postinoculation (dpi). A remarkable increase in the activities of the total soluble phenolics (10-fold) and salicylic acid (16-fold) was detected 5 days after the treatment in foliar extracts of test plants relative to untreated control plants. The results showed that treatment of tomato plants with inactivated viral inoculum led to a significant enhancement of protection against Cucumber mosaic virus attack in a manner that mimics a real pathogen and induces systemic acquired resistance.

  1. In situ determination of growing stages and harvest time of tomato (Lycopersicon esculentum ) fruits using fiber-optic visible-near-infrared (Vis-NIR) spectroscopy.

    Science.gov (United States)

    Yang, Haiqing; Kuang, Boyan; Mouazen, Abdul Mounem

    2011-08-01

    Nondestructive in situ measurement of tomato fruits is essential to determine growing stages and to assist in automatic picking of fruits. This study evaluates the applicability of visible and near-infrared (Vis-NIR) spectroscopy for in situ determination of growing stages and harvest time of three cultivars of tomato fruits. A mobile fiber-type AgroSpec Vis-NIR spectrophotometer (Tec5 Co., Germany) with a spectral range of 350-2200 nm was used to measure tomato spectra in reflection mode. A new growing stage (GS) index defined as the ratio of the current growing age in days to the on-vine duration before harvest in days was proposed. After dividing spectra into a calibration set (70%) and an independent prediction set (30%), spectra in the calibration set were subjected to a partial least squares regression (PLSR) with leave-one-out cross-validation to establish calibration models relating GS to the spectra of tomato fruits. Separate models were developed for each tomato cultivar and compared with a general model that used combined spectra of all three cultivars. The results show that PLSR based on the new GS is successful and robust in predicting the growing stages and harvest time of tomato fruits. Validation of calibration models on the independent prediction set indicates that successful prediction of GS can be achieved using the three models developed separately for each cultivar with a coefficient of determination (R(2)) of 0.91-0.92, root mean square error of prediction (RMSEP) of 0.081-0.097, and residual prediction deviation (RPD) of 3.29-3.70. General calibration using the combined spectra produces good prediction performance, although less accurate than that for the three individual cultivar models. The analysis of regression coefficient plots resulting from PLSR analysis indicates consistent assignment of important wavelengths for individual cultivar spectra and combined spectra. It is concluded that the Vis-NIR PLSR based on GS index can be adopted

  2. Capítulo X: habilidad combinatoria para el carácter producción por planta y sus componentes primarios en un cruzamiento dialélico de siete líneas de tomate "Chonto" Lycopersicon esculentum, Mill

    Directory of Open Access Journals (Sweden)

    García Aura A.

    1994-12-01

    Full Text Available

    An analysis combining ability of traits related with production per plant was carried out using a diallel crossing between different "Chonto" tomato cultivar, Lycopersicon esculentum Mill (seven parents and 21 F1 hybrids from all possible crossing in one direction, according to the methodology proposed by Griffing (1956, selecting experimental method 2, and model 1. Significant differences were found for the two types of combining ability (g.c.a. and s.c.a. on the expression of the characters production per plant, fruits per plant and fruit mean weight. A non-additive gene action was prevailing for the characters production per plant and fruit mean weight. Hybrids Licapal-21 x Raminho, Angela Gigante x 1258 and Angela Gigante x Raminho, exhibited high s.c.a. effects and heigh mean production per plant.

    Se evaluaron siete líneas progenitoras de tomate "Chonto" (Angela Gigante, Licapal 21, Raminho, Olho Roxo, 1258, 1475 Y 1507 y 21 híbridos, sin inclui los recíprocos, mediante el método experimental 2 y modelo tipo 1 propuesto por Griffing (1956. En la expresión de los caracteres producción por planta y peso promedio de fruto se encontraron diferencias significativas tanto para la habilidad combinatoria general (acción génica aditiva como para la habilidad combinatoria específica (acción génica no aditiva, pero con predominio de ésta última. En la expresión del carácter número de frutos por planta se encontró diferencias para los dos tipos de habilidad combinatoria, pero con predominio de la acción génica aditiva. Los híbridos Licapal 21 x Raminho, Angela Gigante x 1258 y Angela Gigante x Raminho exhibieron los mayores efectos de habilidad combinatoria específica y los mayores valores promedios, para el carácter producción por planta.

  3. The Effect of Methyl Jasmonate Vapour on Some Characteristics of Fruit Ripening, Carotenoids and Tomatine Changes in Tomato (Lycopersicon esculentum Mill.

    Directory of Open Access Journals (Sweden)

    Janusz Czapski

    2013-12-01

    Full Text Available Tomato ripening in normal red-fruited cultivar (Fiorin was delayed by treatment with methyl jasmonate (JA-Me vapour. A visual scoring system for describing tomato ripening was used. Surface of fruits exposed to JA-Me vapour, increased in yellow and decreased in red as determined by HunterLab colour meter. JA-Me significantly altered the firmness of fruits after 21 days storage. Vapour of JA-Me enhanced the level of β-carotene in outer part (peel with 3 mm pericarp tissue of fruit, while it had no effect in peeled fruit pericarp. JA-Me treatment decreased the level of lycopene in outer part and pericarp tissue, however, in outer part lycopene content decreased at a higher rate than in pericarp. Amount of tomatine in fruits treated with JA-Me had enhanced four-fold in outer part and by 62% in peeled fruit pericarp as compared with the control.

  4. Pretreated cheese whey wastewater management by agricultural reuse: chemical characterization and response of tomato plants Lycopersicon esculentum Mill. under salinity conditions.

    Science.gov (United States)

    Prazeres, Ana R; Carvalho, Fátima; Rivas, Javier; Patanita, Manuel; Dôres, Jóse

    2013-10-01

    The agricultural reuse of pretreated industrial wastewater resulting from cheese manufacture is shown as a suitable option for its disposal and management. This alternative presents attractive advantages from the economic and pollution control viewpoints. Pretreated cheese whey wastewater (CWW) has high contents of biodegradable organic matter, salinity and nutrients, which are essential development factors for plants with moderate to elevated salinity tolerance. Five different pretreated CWW treatments (1.75 to 10.02 dS m(-1)) have been applied in the tomato plant growth. Fresh water was used as a control run (average salinity level=1.44 dS m(-1)). Chemical characterization and indicator ratios of the leaves, stems and roots were monitored. The sodium and potassium leaf concentrations increased linearly with the salinity level in both cultivars, Roma and Rio Grande. Similar results were found in the stem sodium content. However, the toxic sodium accumulations in the cv. Roma exceeded the values obtained in the cv. Rio Grande. In this last situation, K and Ca uptake, absorption, transport and accumulation capacities were presented as tolerance mechanisms for the osmotic potential regulation of the tissues and for the ion neutralization. Consequently, Na/Ca and Na/K ratios presented lower values in the cv. Rio Grande. Na/Ca ratio increased linearly with the salinity level in leaves and stems, regardless of the cultivar. Regarding the Na/K ratio, the values demonstrated competition phenomena between the ions for the cv. Rio Grande. Despite the high chloride content of the CWW, no significant differences were observed for this nutrient in the leaves and stems. Thus, no nitrogen deficiency was demonstrated by the interaction NO3(-)/Cl(-). Nitrogen also contributes to maintain the water potential difference between the tissues and the soil. Na, P, Cl and N radicular concentrations were maximized for high salinity levels (≥2.22 dS m(-1)) of the pretreated CWW.

  5. 番茄玉米间作对番茄叶片光合特性的影响%Influences of Tomato (Lycopersicon esculentum )/Maize (Zea mays L.) Intercropping on Photosynthetic Characteristics of Tomato Leaves

    Institute of Scientific and Technical Information of China (English)

    董华芳

    2012-01-01

    [Objective] The purpose was to discuss tomato leaves photosynthetic characteristics under tomato/maize intercropping. [Method] The 3 tomato varieties ( 6629, Jinaihong and Yuxing) and 2 maize varieties(Tiannuo8hao and Tianmi2hao) were used to study the influences of tomato/ maize intercropping on photosynthetic characteristics of tomato leaves by using the Ii-6400 portable photosynthesis system. [Result] The results showed that there were highly significant differences on the net photosynthetic rate, conductance to H2O and the intercellular CO2 concentration of tomato leaves between tomato/maize intercropping and monoculture. The net photosynthetic rate and conductance to H2O of tomato leaves under tomato/maize intercropping were decreased significantly compared with monoculture. The intercellular C02 concentration of tomato leaves under tomato/maize intercropping was increased significantly compared with monoculture. But the influences to 3 varieties were different, the influences to 6629 was lower relatively, and the Jinaihong and Yuxing were larger relatively. [ Conclusion] The study provides a theoretical basis for tomato cultivation and management.%[目的]探讨番茄(Lycopersicon esculentum)玉米(Zea mays L.)间作条件下的番茄叶片光合特性.[方法]以6629、金矮红、豫星3个番茄品种和甜糯八号、田蜜二号2个玉米品种为试验材料,将番茄与玉米进行间作,利用Li-6400便携式光合仪在自然光照条件下,测定番茄叶片的净光合速率、气孔导度等参数.[结果]番茄玉米间作降低了番茄叶片的净光合速率和气孔导度,增大了其叶片胞间CO2浓度,与单作相比,差异均达到了极显著水平.不同番茄品种变化幅度不同,其中6629受影响较小,金矮红和豫星受影响较大.[结论]该研究可为番茄栽培管理提供理论依据.

  6. Effects of Oscillating Pollination on Fruit Setting, Production and Quality of Tomato(Lycopersicon esculentum) in Spring%振荡授粉在春番茄坐果及产量品质上的效果

    Institute of Scientific and Technical Information of China (English)

    李军; 孔庆祥; 刘凤军; 王先龙; 高娟

    2012-01-01

    [Objective] The aim was to discuss the effects of oscillating pollination on fruit setting, production and quality of tomato in spring, and find out a new no hormone pollination method for tomato. [ Method ] The rate of fruii setting, speed of fruit setting, speed of fruit enlargement, production and quality of tomato treated by oscillating pollination, applying Fanqieling and natural pollination were comparatively analyzed in spring. [ Result] The results showed that oscillating pollination could improve setting rate and growth of fruit. The fruit setting rate was the highest in three treatments on first truss fruit after applying Fanqieling, while it was the highest on second and third truss after oscillating pollination. The bunch uniformity, fruit enlargement speed, early - stage yield were higher with applying Fanqieling than those with oscillating pollination. The content of solution sugar was significant lower with oscillating pollination than that with natural pollination, while the content of lycopene was significant higher. [ Conclusion ] Oscillating pollination can replace Fanqieling to promote tomato fruit setting, and realize no hormone tomato production.%[目的]探讨振荡授粉对春番茄(Lycopersicon esculentum)坐果、产量和品质的影响,以寻找一种番茄无激素授粉的新方法.[方法]以春番茄为材料,对比振荡授粉、施用番茄灵和自然授粉对其坐果率、坐果速度、番茄果实膨大速度、产量和品质的影响.[结果]与自然授粉相比,振荡授粉与施用番茄灵增加了番茄的坐果率,促进了果实发育.第1穗花坐果率施用番茄灵较好,在第2、3穗花上振荡授粉较好.振荡授粉在坐果整齐度、果实膨大连度及早期产量等方面不如施用番茄灵.振荡授粉的番茄可溶性糖含量稍差于自然授粉,但番茄红素含量显著升高.[结论]振荡授粉可以代替番茄灵促进番茄坐果,实现无激素番茄生产.

  7. Utilização do Ozônio no Tratamento Pós-Colheita do Tomate (Lycopersicon esculentum Mill

    Directory of Open Access Journals (Sweden)

    Rogério Simão

    2011-06-01

    Full Text Available O armazenamento de hortaliças no período pós-colheita é fator preocupante na logística de produtos perecíveis no ramo do agronegócio. Este artigo tem como objetivo mostrar o emprego do gás Ozônio (O3 para o aumento de vida de prateleira de frutos de tomate a fim de mantê-los adequados por mais tempo para a comercialização. Exemplares da cultivar Débora foram tratados com 1 ppm (vol/vol de Ozônio durante 24 horas a 25°C e 65% de umidade relativa. Qualidades físicas como perda de massa, estádio de maturação, injúria e senescência foram avaliadas após 15 dias de armazenamento. Comparados com uma amostra de controle, os frutos tratados com Ozônio apresentaram menos perda de massa, menor porcentagem de injúrias causada por fungos e maior retardamento no apodrecimento, mas pouca diferença quanto ao nível de maturação.

  8. 秘鲁番茄与栽培番茄回交一代的获得及其染色体片段分析%Obtain and Chromosome Fragments Analysis of BC1 Populations between Ly-copersicon esculentum Mill. and L. peruvianum

    Institute of Scientific and Technical Information of China (English)

    赵鹤; 王孝宣; 杜永臣; 高建昌; 国艳梅

    2013-01-01

      In this paper,cultivated tomato Lycopersicon esculentum Mill. 1052 was used as female parent and wild tomato L. peruvianum LA3858 was used as male parent.One F1 hybrid was obtained by embryo rescuing method.The hybrid was backcrossed with Lycopersicon esculentum Mill. 1052 to generate BC1 progeny.And 6 single plants of backcross F1 species hybrids were obtained. Moreover,129 primers of CAPS,SSR and InDel on 12 chromosomes were used to analyze chromosome fragments of BC1. The results showed that these 6 BC1 plants contained the whole genome of L. peruvianum LA3858.%  以栽培番茄1052为母本,以秘鲁番茄LA3858为父本进行杂交,通过幼胚挽救培养获得了1株F1植株,再以栽培番茄1052为母本进行回交,通过幼胚挽救获得了6株BC1单株,利用129个分布于12条染色体上的CAPS、SSR和InDel标记对BC1单株进行染色体片段分析,结果表明6株BC1单株中12条染色体含有的秘鲁番茄片段涵盖了秘鲁番茄LA3858的整个基因组。

  9. Growing Tomato (Lycopersicon esculentum L.) in Nematode ...

    African Journals Online (AJOL)

    Infested Soil and the Pest Implications in Poorly Managed. Post-harvested Fields ... In this study, the effect of Procarvian carpensis manure at a rate of 5tons/ha and the balanced ... nematode in Tabora environmental condition. Furthermore ...

  10. Comparison of regeneration potentials in tissue cultures of primitive and cultivated tomato species (Lycopersicon sp.

    Directory of Open Access Journals (Sweden)

    M. Lech

    2014-01-01

    Full Text Available Regeneration capacities of two tomato cultivars: Potentat and Rutgers, and of three accessions of wild tomato species: Lycopersicon peruvianum PI 128650, L. peruvianum var. dentatum PI 128655 and L. glandulosum were studied using an universal medium suitable for regeneration of those plants from leaf pieces in tissue culture. Fragments of leaf blades were taken from plants raised in greenhouse conditions and placed on a modified MS medium containing 0.3 mg/l IAA and 3.0 mg/l BAP solidified with 1% agar. The explants were transferred every 4-5 weeks on fresh medium of the same composition. It was shown that all the three primitive tomato species revealed much higher multiplication coefficients than the two cultivars. Appropriate values were: 11 - for L. glandulosum, 8 - for L. peruvianum, 7 - for L. peruvianum var. dentatum, 4 - for L. esculentum cv. Potentat and 2 - cv. Rutgers. Completely regenerated plants were obtained from all the tested species, but organogenesis occurred almost two weeks earlier in wild tomatoes than in the culitivated varieties of L. esculentum.

  11. EVALUACIÓN DE NUEVOS CULTIVARES DE TOMATE (Lycopersicon esculentum MILL.) EN LOS PERÍODOS TEMPRANO Y ÓPTIMO DE SIEMBRA EN EL OCCIDENTE DE CUBA

    OpenAIRE

    Moya, C.; A. Oliva; Marta Álvarez; C. Morales; Marilyn Florido; Dagmara Plana

    2001-01-01

    Se evaluaron siete nuevas variedades procedentes de distintos programas de mejoramiento genético de esta especie, una variedad local y un material semi-silvestre con cierto grado de domesticación, los cuales se compararon con la variedad Campbell-28, considerada entre los materiales de mayor plasticidad utilizados en Cuba. Las siembras se ejecutaron el 4 de septiembre en la campaña 1997-1998 y el 3 de diciembre en la campaña 1998-1999, esta última con el objetivo de medir el potencial product...

  12. The inheritance of chilling tolerance in tomato (Lycopersicon spp).

    NARCIS (Netherlands)

    Venema, J.H.; Linger, P.; Heusden, van A.W.; Hasselt, van P.R.; Bruggemann, W.

    2005-01-01

    During the past 25 years, chilling tolerance of the cultivated (chilling-sensitive) tomato Lycopersicon esculentum and its wild, chilling-tolerant relatives L. peruvianum and L. hirsutum (and, less intensively studied, L. chilense) has been the object of several investigations. The final aim of thes

  13. The inheritance of chilling tolerance in tomato (Lycopersicon spp.)

    NARCIS (Netherlands)

    Venema, JH; Linger, P; van Heusden, AW; van Hasselt, PR; Brueggemann, W

    2005-01-01

    During the past 25 years, chilling tolerance of the cultivated (chilling-sensitive) tomato Lycopersicon esculentum and its wild, chilling-tolerant relatives L. peruvianum and L. hirsutum (and, less intensively studied, L. chilense) has been the object of several investigations. The final aim of thes

  14. 种子低温处理对低夜温下番茄幼苗生长的影响%Effects of Tomato( Lycopersicon esculentum) Seeds Treated with Low Temperature on Their Growth of Seedlings under Low Night Temperature

    Institute of Scientific and Technical Information of China (English)

    陈丽平; 赵方贵; 杜广宾; 李敏; 王富

    2012-01-01

    [目的]研究番茄(Lycopersicon esculentum)种子低温处理对低夜温条件下番茄幼苗生长的影响,探讨番茄幼苗在低夜温下的抗冷性,为低温下获得高质量的番茄幼苗提供理论依据.[方法]以草炭与珍珠岩(2∶1)为育苗基质,番茄L402种子为供试材料.先将番茄402种子浸种6h,然后分别于0、4、8和16℃低温条件下进行6、12和24h低温处理,对照为0h处理.之后,于16℃下静置0.5h,再后在25 ~30℃下催芽,播种于72穴育苗盘中.育苗温度为18 ~ 23℃(白天)/6~12℃(夜间).培养至3片真叶时测定幼苗的各项生长指标(干重、鲜重、根冠比)和生理指标[根系活力、超氧化物歧化酶(SOD)活性及叶绿素、脯氨酸、可溶性蛋白、可溶性糖和丙二醛(MDA)含量].[结果]种子低温处理能促进低夜温下番茄幼苗的生长,提高幼苗叶绿素含量、可溶性蛋白和脯氨酸的含量,并能增加番茄幼苗在低夜温下的根系活力和SOD活性,同时降低了MDA含量,其中0℃下处理6h对番茄幼苗在低夜温下的抗冷性提高最大.[结论]种子低温处理能增加番茄幼苗对低夜温的抵抗能力,有利于提高低温季节番茄幼苗的质量.%[ Objective] The purpose was to study the effects of seed treated with low temperature on their growth of seedlings at low night temperature, discuss their resistance to cool under low night temperature, provide a theoretical basis for obtaining high quality tomato seedlings under low temperature. [Method] Tomato(L402) seeds soaking 6 h with water were treated 6, 12, and 24 h in 0(CK), 4, 8, and 16 ℃ respectively. Then after stewing them 0.5 h under 16 ℃, they were accelerating germination ane sowing in raising seedling dish with 72 holes under 25-30 ℃. Raising seedling temperature was 18 — 23 ℃ at day and 6 - 12 ℃ at night, and raising matrix was mixture of ratio of peat and perlite tailings 2: 1 respectively. All kinds of growth indices( Dry weight

  15. Adaptive evolution of the water stress-induced gene Asr2 in Lycopersicon species dwelling in arid habitats.

    Science.gov (United States)

    Frankel, Nicolas; Hasson, Esteban; Iusem, Norberto D; Rossi, Maria Susana

    2003-12-01

    The Asr2 gene encodes a putative transcription factor that is up-regulated in leaves and roots of tomato plants exposed to water-deficit stress. This gene was first cloned and characterized in a cultivar of commercial tomato (Lycopersicon esculentum cv. Ailsa Craig). In this work, we report the complete coding sequences of the orthologous Asr2 genes in six wild tomato lineages: L. hirsutum, L. cheesmanii, L. esculentum v. cerasiforme, L. chilense, L. peruvianum v. humifusum and L. peruvianum f. glandulosum. Estimates of the Ka/Ks ratio (omega) in pairwise comparisons within the genus Lycopersicon were equal or greater than 1 (a signature of adaptive evolution) when involving L. chilense and L. peruvianum v. humifusum. Interestingly, these two species are distinct from the others in their adaptation to dry habitats. We also mapped the detected substitutions onto a phylogenetic tree of the genus Lycopersicon. Remarkably, there are two and three amino acid substitutions, which contrast with the absence of synonymous substitutions along the terminal branches leading to L. chilense and L. peruvianum v. humifusum, respectively. Likelihood ratio tests confirmed that omega values in the branches leading to these species are significantly different from the remaining branches of the tree. Moreover, inferred changes in the branches leading to these species that inhabit dry areas are nonconservative and may be associated with dramatic alterations in ASR2 protein conformation. In this work, we demonstrate accelerated rates of amino acid substitutions in the Asr2 gene of tomato lineages living in dry habitats, thus giving support to the hypothesis of adaptive Darwinian evolution.

  16. 植物声频控制技术对番茄生长性状、叶绿素及内源激素影响的研究%Effects of Plant Acoustic Frequency Technology on the Growth Traits, Chlorophyll Content and Endogenous Hormones of Lycopersicon esculentum

    Institute of Scientific and Technical Information of China (English)

    孟庆午; 周清; 郑劭婧; 高杨

    2012-01-01

    植物声频控制技术是利用作物与声波的协同关系,对作物发射一定波长和频率的声波,以促进作物生长及提高产量、质量的农业新技术.试验以番茄为试验材料,研究植物声频控制技术对番茄生长性状、叶绿素含量及内源激素生长素、赤霉素、玉米素核苷和脱落酸含量的影响.结果表明,植物声频控制技术能显著促进番茄的生长,可提高产量与叶片叶绿素的含量,还能刺激番茄内源激素生长素的分泌,并且刺激作用具有一定的时间积累效应.%Plant acoustic frequency technology was a new agricultural technology using certain wavelengths and frequencies of sound on the crops according to the sound and crops cooperative relations to increase the growth, yield and the quality of the crops. The effects of plant acoustic frequency control technology on Lycopersicon esculentum Mill, growth traits, chlorophyll content and endogenous plant hormones auxin, gibberellin, zeatin riboside and abscisic acid content were studied. The results showed that the plant acoustic frequency control technology could significantly promote the growth, yield and chlorophyll content of L. esculentum, stimulate the producing of endogenous hormone ghrelin secretion, which has time accumubation effect.

  17. Mycorrhizal fungi in tomatoplantless Lycopersicon esculentum Miller Efecto de hongos micorrizogenos en tomate Lycopersicon esculentum Miller

    Directory of Open Access Journals (Sweden)

    Sánchez de Prager Marina

    1995-12-01

    Full Text Available

    Two soils from Cauca Department (Colombia were pasteurized; soil analysis showed that they contained 3.0 and 9.0 ppm P, pH 4.8 and 6.2, respectively. They were fertilized with 0, 30 and 60 kg P/ha. Tomato plantlets var. Chonto Santa Cruz were inoculated with 11 VAM isolates of the CIAT VAM fungal germplasm and one VAM native fungi. Plantlets were grown in pots. Fifty two days after plants were harvested and height, dry matter, tissue analysis tor N, P, K, Ca, Mg and B was performed; fungus infection and health were determined. The statistical analysis showed that the major differences in this bioassay were due to a type of soils and their nutritional conditions. Plant dry matter and height increased with the increased levels of fertilizer. Best grow results were obtained with 60 kg P/ha. Dry matter, heigth and nutrient uptake (N, P, K, Ca and B were significantly different between introduced VAM and the native fungi. VAM fungi isolates had different behavior in each soil. This confirmed the soil influence in the symbiosis effectiveness.

    En condiciones de invernadero, en dos suelos del Departamento del Cauca (Colombia, previamente pasteurizados, con contenidos de P de 3.0 y 9.0 ppm y pH de 4.8 y 6.2 respectivamente, sujetos a fertilización con O, 30 y 60 kg de P/ha, se probaron 11 cepas de hongos micorrizógenos provenientes de diferentes regiones del país y con la flora nativa de cada suelo. Las plantas se cosecharon a los 52 días de transplante y se les determinó altura, materia seca, análisis foliar de N, P, K. Ca, Mg y B, infección por hongos micorrizógenos y sanidad del cultivo. Las mayores diferencias en este ensayo se debieron al tipo de suelo y sus condiciones nutricionales. La materia seca y altura de las plantas aumentaron en ambos suelos, al incrementarse la fertilización, los mejores resultados en desarrollo vegetativo y reproductivo ocurrieron con 60 kg de P/ha. En materia seca, altura y absorción de N, P, K, Ca, y B, hubo diferencias altamente significativas entre cepas introducidas y flora nativa. Las cepas variaron en su comportamiento en los 2 suelos, lo cual corrobora la alta influencia de este factor en la efectividad de la simbiosis.

  18. Effect of acclimation to suboptimal temperature on chilling-induced photodamage : comparison between a domestic and a high-altitude wild Lycopersicon species

    NARCIS (Netherlands)

    Venema, JH; Villerius, L; van Hasselt, PR

    2000-01-01

    Plants of a domestic (Lycopersicon esculentum [L.] Mill. cv. Abunda) and a high-altitude wild Lycopersicon species (L. peruvianum Mill. LA 385) were grown at near-optimal (25/20 degrees C) or suboptimal (16/14 degrees C) temperature. Leaf discs from just fully expanded leaves were exposed to an

  19. Effect of acclimation to suboptimal temperature on chilling-induced photodamage : comparison between a domestic and a high-altitude wild Lycopersicon species

    NARCIS (Netherlands)

    Venema, JH; Villerius, L; van Hasselt, PR

    2000-01-01

    Plants of a domestic (Lycopersicon esculentum [L.] Mill. cv. Abunda) and a high-altitude wild Lycopersicon species (L. peruvianum Mill. LA 385) were grown at near-optimal (25/20 degrees C) or suboptimal (16/14 degrees C) temperature. Leaf discs from just fully expanded leaves were exposed to an irra

  20. The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato (Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles.

    Science.gov (United States)

    Alharby, Hesham F; Metwali, Ehab M R; Fuller, Michael P; Aldhebiani, Amal Y

    2016-11-01

    Five cultivars of tomato having different levels of salt stress tolerance were exposed to different treatments of NaCl (0, 3 and 6 g L(-1)) and ZnO-NPs (0, 15 and 30 mg L(-1)). Treatments with NaCl at both 3 and 6 g L(-1) suppressed the mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) genes in all cultivars while plants treated with ZnO-NPs in the presence of NaCl, showed increments in the mRNA expression levels. This indicated that ZnO-NPs had a positive response on plant metabolism under salt stress. Superior expression levels of mRNA were observed in the salt tolerant cultivars, Sandpoint and Edkawy while the lowest level was detected in the salt sensitive cultivar, Anna Aasa. SDS-PAGE showed clear differences in patterns of protein expression among the cultivars. A negative protein marker for salt sensitivity and ZnO-NPs was detected in cv. Anna Aasa at a molecular weight of 19.162 kDa, while the tolerant cultivar Edkawy had two positive markers at molecular weights of 74.991 and 79.735 kDa.

  1. Cultivars

    Directory of Open Access Journals (Sweden)

    Oleg Blum

    2011-01-01

    Full Text Available Some modern agrochemicals with antioxidant potential were tested for their protective effect against ozone injury using clover and tobacco ozone-sensitive cultivars as model plants subjected to ambient ozone at two sites (Kyiv city in Ukraine and Szarów village in Poland. All used agrochemicals showed partial protective effects against ozone injury on clover and tobacco. Conducted studies confirmed the effectiveness of modern fungicides belonging to strobilurin group as protectants of sensitive crops against ozone damage. The effectiveness of new growth regulators “Emistym C” and “Agrostymulin” was showed for the first time. Out of the studied agrochemicals, fungicide “Strobi” and natural growth regulator “Emistym C” demonstrated the best protective effects. These agrochemicals present promise for further studies of their possible utilization for enhancement of ozone tolerance of sensitive crops.

  2. DOES LYCOPERSCON ESCULENTUM (TOMATO)

    African Journals Online (AJOL)

    Dr. Ajani

    Respective values for fibroblast and angiogenesis of the scar tissues in ... Key words: Wound healing, Lycoperscon esculentum, Wound contraction, fibroplasia ... We sought to investigate the role of L. esculentum in the management of wound.

  3. Introgressions of Lycopersicon pennellii improve growth and development of greenhouse tomatoes

    NARCIS (Netherlands)

    Manga Owona, S.

    2005-01-01

    This thesis focused on the possibilities to improve greenhouse tomato (Lycopersicon esculentum cv. Moneymaker) in terms of yield. The domestic tomato has a very narrow genetic base which makes breeding for better performance a difficult task. The wild, crossable relatives of tomato present the possi

  4. Introgressions of Lycopersicon pennellii improve growth and development of greenhouse tomatoes

    NARCIS (Netherlands)

    Manga Owona, S.

    2005-01-01

    This thesis focused on the possibilities to improve greenhouse tomato (Lycopersicon esculentum cv. Moneymaker) in terms of yield. The domestic tomato has a very narrow genetic base which makes breeding for better performance a difficult task. The wild, crossable relatives of tomato present the

  5. Capítulo VI: evaluación de la resistencia al pasador del fruto de tomate Neoleucinodes elegantalis (Gueneé en materiales L. hirsutum Humb y Bonpl y L. pimpinellifolium (Just mill y su transferencia a materiales cultivados de tomate L. esculentum Mill

    Directory of Open Access Journals (Sweden)

    Salinas Helbert

    1994-12-01

    Full Text Available

    La investigación tuvo como objetivo estudiar el ciclo de vida del pasador  del fruto del tomate, N. elegantalis y evaluar la resistencia genética en diferentes accesiones de Lycopersicon y en poblaciones derivadas de cruzamientos interespecíficos entre L. esculentum, L. pimpinellifolium y L. hirsutum. La evaluación se realizó en condiciones de campo, utilizando un diseño de bloques completos al azar, con cuatro repeticiones. Se midieron los siguientes caracteres: estados del ciclo de vida, número de posturas, cantidad de frutos dañados, número de perforaciones de entrada, número de larvas por fruto e intensidad del daño. Se determinó el ciclo de vida del insecto plaga. Las especies silvestres fueron calificadas como muy resistentes o resistentes. Las variedades comerciales fueron calificadas como susceptible o medianamente susceptibles. Las poblaciones segregantes provenientes de los cruzamientos interespecíficos fueron calificados como resistentes o ligeramente susceptibles, indicando la posibilidad de introgresión genética de la resistencia. El insecto plaga  tiene mayor preferencia por fenotipos con frutos de mayor peso promedio y pericarpio duro.

    The research was carried out to study the life cicle of N. elegantalis, and the identification of resistence to the insect among Lycopersicon accessions and derivated populations from crossing between L. esculentum, L. pimpinellifolium and L. hirsutum. The life cicle of N. elegantalis was determinated. The wild species L. hirsutum and L. pimpinellifolium were very resistant and resistant, respectively. The Lycopersicon cultivars were susceptibles and derivated populationes from interspecific crossing were resistant or intermedium susceptible. There were associations between the fruit size, fruit firmness, fruit weight and susceptible expression in the plants from crossing between L. hirsutum, L. pimpinellifolium and commercial cultivars.

  6. 过表达LeNLP4转录因子提高番茄抗低温胁迫能力%Overexpression of LeNLP4 Transcription Factor Improved the Chilling Toler-ance of Tomato (Lycopersicon esculentum Mill.)

    Institute of Scientific and Technical Information of China (English)

    左衍秋; 马娜娜; 梁晓庆; 岳萌萌; 孟庆伟

    2014-01-01

    NAC (NAM-ATAF1,2-CUC2) family members play important roles in various environmental re-sponses. In order to investigate the function of LeNLP4 under chilling stress, LeNLP4 was isolated from tomato (Lycopersicon esculentum) and transgenic plants were obtained. qRT-PCR confirmed that the expression of LeNLP4 was induced by chilling stress. Compared with the wild type (WT), transgenic tomato plants have higher growth increment and the maximal photochemistry efifciency of photosystem II (Fv/Fm), higher capabili-ty for scavenging hydrogen peroxide (H2O2) and superoxide radical (O2.-), higher ascorbate peroxidase (APX) and superoxide dismutase (SOD), and lower malondialdehyde (MDA) content and the relative electric conduc-tivity (REC) under chilling treatment. Expression level of SlCBF1 in transgenic lines was higher than that in WT. These results indicated that overexpression of LeNLP4 enhanced chilling tolerance of transgenic tomato plants.%NAC (NAM-ATAF1,2-CUC2)转录因子在植物胁迫响应中起重要作用。为了探讨LeNLP4基因在番茄抗低温胁迫中的功能,分离了番茄LeNLP4转录因子基因,并获得转正义LeNLP4基因番茄植株。荧光定量PCR分析表明, LeNLP4的表达受低温诱导。与野生型植株相比,在4℃胁迫下转基因植株具有较高的生长量和光系统II (PSII)最大光化学效率(Fv/Fm)、过氧化氢(H2O2)和超氧阴离子(O2.-)清除速率、抗坏血酸过氧化物酶(APX)和超氧化物歧化酶(SOD)活性,以及较低的丙二醛(MDA)含量和相对电导率(REC)。过表达株系中SlCBF1的表达高于野生型。上述结果表明, LeNLP4的过表达提高了转基因番茄抗低温胁迫能力。

  7. İn Vitro Koşullarda Domates (Lycopersicon esculentum Mill. Bitkisinde Hipokotil ve Kotiledon Eksplantlarından Kallus ve Sürgün Oluşumu

    Directory of Open Access Journals (Sweden)

    Emel YILMAZ

    2015-01-01

    Full Text Available In this study, the effects of various plant growth regulators and culture conditions on the callus induction and shoot regeneration in M-28 hybrid cultivar of tomato have been evaluated. The seeds were germinated in half strength (½ Murashige-Skoog (MS medium and the cotyledon (5x5 mm and hypocotyl (5 mm segments of the 10 day old seedlings were cultured on semi-solide MS medium containing different concentrations and combinations of plant growth regulators. The best result on callus formation, both explants (cotyledon and hypocotyl and both culture conditions (photoperiodic condition at 16 hours light8 hours dark ve 25 days dark condition after photoperiodic condition, was found MS medium supplemented with 1 mg/l BAP+1 mg/l NAA (%100 callus formation. At the adventitious shoot induction, under dark condition after photoperiodic condition, the best results were obtained from MS medium supplemented with 2 mg/l Kin+0.2 mg/l IAA, only 2 mg/l BAP and only 3 mg/l TDZ and both explants (regenerated explant from %72 to %100. The highest number of shoots regenerated per explant (17.30±5.72 number, was found under dark condition after photoperiodic condition at MS medium supplemented with TDZ of cotyledon explants. The highest adventitious shoot length (33.00±0.57 mm and the number of leaf in shoot (3.00±0.00 number were obtained at MS supplemented with 1 mg/l BAP+1 mg/l NAA of hypocotyl explants and photoperiodic conditions

  8. 根围促生细菌(PGPR)蜡质芽胞杆菌AR156对番茄的诱导耐旱性研究%Enhancement of Tomato(Lycopersicon esculentum) Tolerance to Drought Stress by Plant-Growth-Promoting Rhizobacterium (PGPR) Bacillus cereus AR156

    Institute of Scientific and Technical Information of China (English)

    王春娟; 郭亚辉; 王超; 刘红霞; 牛冬冬; 王云鹏; 郭坚华

    2012-01-01

    根围促生细菌(PGPR)蜡质芽胞杆菌(Bacillus cereus)AR156是具有很高应用前景的生防菌剂,为了研究其对番茄(Lycopersicon esculentum)的诱导耐旱机理,本研究通过检测干旱胁迫条件下叶片相对含水量、丙二醛(MDA)含量、相对电导率、叶绿素含量和根系还原能力,抗氧化酶活性以及胞质抗坏血酸还原酶基因(cAPX)、单脱氢抗坏血酸还原酶基因(MDHAR)和核酮糖-1,5-二磷酸羧化酶(Rubisco)小亚基基因(rbcS)的表达情况,从表型、生理水平和分子水平上进行阐释.研究发现,干旱胁迫条件下AR156培养上清诱导后,番茄叶片相对含水量为82%,与对照组相比提高了44%;MDA含量为2.2x10-3μmol/g FW,降低了21.4%,相对电导率为56.0%,降低了28.2%;叶绿素a、叶绿素b和总叶绿素含量分别增加了16%、20%和20.2%;根系还原强度为0.336 mg/g/h,是对照组的两倍;另外,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性也显著提高;同时,分子水平的检测发现rbcS在干旱处理过程中—直保持较高水平,cA PX和MDHAR的表达在干旱处理后期也显著高于对照组;并且干旱胁迫处理20 d后重新浇水24 h,番茄存活率达到90.91%,比对照组高1.6倍.结果表明,AR156培养上清可以通过保护细胞膜的完整性、维持较高水平的光合效率和提高抗氧化酶活性来提高番茄的系统耐旱性,初步阐释了PGPR AR156的诱导耐旱机理,为AR156作为优良生物类耐旱诱导因子提供了理论依据.%Plant-growth promoting rhizobacterium (PGPR) Bacillus cereus AR156 is a potential bio-pesticide. In order to study the mechanism of drought tolerance in tomato (Lycopersicon esculentum) induced by B. cereus AR156, we tested relative water content, malondialdehyde (MDA) content, relative electrical conductivity and chlorophyll content of leaves and recovery intension of roots, the antioxidant enzymes activities and the

  9. Chloroplast DNA evolution and phylogenetic relationships in Lycopersicon.

    Science.gov (United States)

    Palmer, J D; Zamir, D

    1982-08-01

    Chloroplast DNA was purified from 12 accessions that represent most of the species diversity in the genus Lycopersicon (family Solanaceae) and from 3 closely related species in the genus Solanum. Fragment patterns produced by digestion of these DNAs with 25 different restriction endonucleases were analyzed by agarose gel electrophoresis. In all 15 DNAs, a total of only 39 restriction site mutations were detected among 484 restriction sites surveyed, representing 2,800 base pairs of sequence information. This low rate of base sequence change is paralleled by an extremely low rate of convergent change in restriction sites; only 1 of the 39 mutations appears to have occurred independently in two different lineages. Parsimony analysis of shared mutations has allowed the construction of a maternal phylogeny for the 15 accessions. This phylogeny is generally consistent with relationships based on morphology and crossability but provides more detailed resolution at several places. All accessions within Lycopersicon form a coherent group, with two of the three species of Solanum as outside reference points. Chloroplast DNA analysis places S. pennellii firmly within Lycopersicon, confirming recent studies that have removed it from Solanum. Red-orange fruit color is shown to be a monophyletic trait in three species of Lycopersicon, including the cultivated tomato, L. esculentum. Analysis of six accessions within L. peruvianum reveals a limited amount of intraspecific polymorphism which, however, encompasses all the variation observed in L. chilense and L. chmielewskii. It is suggested that these latter two accessions be relegated to positions within the L. peruvianum complex.

  10. 1-甲基环丙烯采后处理对樱桃番茄果实成熟过程的影响%Effect of 1-Methylcyclopropene Post-harvest Treatment on Ripening Process in Cherry Tomato Fruit (Lycopersicon esculentum var.cerasiforme)

    Institute of Scientific and Technical Information of China (English)

    Arnold M.Opiyo; 应铁进

    2005-01-01

    研究了不同浓度(0、0.035、0.07和0.11μL/L)的乙烯受体竞争性抑制剂1-甲基环丙烯(1-MCP)采后处理对绿熟期樱桃番茄的乙烯合成、果实软化、果实色素(叶绿素、茄红素、β-胡萝卜素)含量消长的影响.0.07 μL/L及其以上浓度的1-MCP降低了前期乙烯合成,同时推迟了乙烯释放高峰,但0.035 μL/L浓度的1-MCP处理并不能抑制内源乙烯合成.1-MCP显著延迟了果实软化和叶绿素降解,但并不影响这两个过程的启动.茄红素合成的启动和积累均受到了1-MCP抑制,而1-MCP并不推迟β-胡萝卜素合成的启动,只抑制其积累.这些结果提示了乙烯调节成熟生理过程的不同机制.对于绿熟期的樱桃番茄,0.07~0.11μL/L的1-MCP是实用的有效处理浓度.1-MCP有效浓度可能用于了解果实的乙烯受体水平和乙烯敏感性.%The responses of cherry tomato(Lycopersicon esculentum var. cerasiforme) fruits to post-harvest treatment with 1-MCP were investigated.The maturity stage at which 1-MCP application is most effective in delaying the ripening process was determined, and then the effects of different concentrations (0, 0.035, 0.07 and 0.11 μL/L) of 1-MCP on ethylene production, fruit softening, chlorophyll, lycopene and carotenoids contents of mature green (MG)cherry tomato fruits were assessed. 1-MCP at 0.07 and 0.11 μL/L reduced fruit C2H4 production, delayed the C2H4 peak at ambient temperature. Although 1-MCP at 0.035 μL/L was effective in retarding fruit ripening,it did not suppress endogenous ethylene production.Fruit softening was suppressed by 1-MCP, but its initiation was not affected by 1-MCP. The rate of chlorophyll degradation and its pattern of change with time,and the initiation of lycopene biosynthesis as well as its accumulation were all affected by 1-MCP, but only the accumulation of carotenoids was suppressed. Accumulation of lycopene and carotenoids was almost permanently hampered by 1-MCP at 0.07 μL/L or

  11. Effects of water and nitrogen coupling on the fruit yield and quality of Lycopersicon esculentum in the middle reaches of the Heihe River%水氮耦合对黑河中游加工番茄产量和品质的影响

    Institute of Scientific and Technical Information of China (English)

    郭艳红; 张凯; 陈延昭; 韩国君; 陈年来

    2014-01-01

    试验以加工番茄‘里格尔87-5’为材料,采用二因素随机区组设计,设充分灌溉(CK)、轻度亏缺灌溉和中度亏缺灌溉3个水分梯度,设低氮、中氮、高氮3个氮素水平,探讨土壤水分亏缺的程度和氮素水平对番茄果实产量和品质的影响。结果表明:番茄果实产量为轻度亏水及中氮组合处理下最高,中度亏缺灌溉、施氮量过低或过高都不利于番茄果实产量的形成;中度亏水高氮组合显著提高番茄果实干物质和可溶性固形物含量;中氮水平下轻度和中度亏水处理均能显著提高果实番茄红素和果实抗坏血酸含量。综合认为,最优的水氮配比为轻度亏水中氮水平和中度亏水中氮水平的组合。%To promote the development of water-saving agriculture in the midstream of Heihe,we con-ducted an experiment on Lycopersicon esculentum cv.‘Riegel 87-5’to study the effects of water and nitro-gen coupling on the open field of tomato in Linze County in the midstream of Heihe.Double factors ran-domized block design was adopted and three water gradients,full irrigation,light deficit irrigation and me-dium defficit irrigation were arranged.Meanwhile,three nitrogen levels,low nitrogen,medium nitrogen and high nitrogen were also set to study the effects of water stress and nitrogen level on the fruit yield and quality of tomato.The results showed that tomato had the highest yield in field with light hydropenia and medium nitrogen.Medium deficit irrigation,low or high nitrogen level were not good for the fruit yields of tomato.Applying medium deficit irrigation together with high nitrogen could remarkably increase the con-tent of dry matter and soluble solids of tomato.Applying light or medium deficit irrigation under the condi-tion of medium nitrogen could greatly increase the content of lycopene and ascorbic acid of the fruit.In summary,the best water-nitrogen formulas were light deficit irrigation with medium

  12. Análise dialélica da capacidade combinatória de cultivares de tomateiro Diallel analysus of the combining ability of tomato cultivars

    Directory of Open Access Journals (Sweden)

    Antonio Teixeira do Amaral Júnior

    1996-01-01

    Full Text Available Os cultivares de tomateiro (Lycopersicon esculentum Mill. Ângela I.5100, Floradade, IPA-05, IPA-06, Jumbo e Santa Clara, e os 15 possíveis híbridos F1s foram avaliados quanto à produção de frutos comerciáveis (PFC, espessura média da polpa (EMP, número médio de lóculos por fruto (NML e teor de sólidos solúveis (TSS, com o objetivo de identificar parentais e híbridos promissores para programas de melhoramento. A análise dialélica, com base no método de Griffing, revelou a predominância dos efeitos gênicos não aditivos para o caráter PFC, ao contrário de EMP, enquanto NML acusou equivalência para os efeitos aditivos e não aditivos. Em decorrência das estimativas da capacidade geral de combinação (Gi os cultivares Ângela I.5100, IPA-05 e IPA-06 são indicados para o desenvolvimento de programas de melhoramento.The tomato (Lycopersicon esculentum Mill. cultivars Ângela I.5100, Floradade, IPA-05, IPA-06, Jumbo and Santa Clara and their diallelic crosses were evaluated for commercial fruit weight (CFW, average thickness of the pule (ATP, average number of loculi per fruit (NLF and content of soluble solids (CSS. The objective was to identify the superior progenitors and the promising hybrids for plant breeding programs. The diallel analysis based on Griffings Methodology, showed a prevailing non-additive genic effects for CFW, the opposite being observed for ATP meanwhile NLF showed an equal value for additive and non-additive effects. Based on general combining ability estimates, the cultivars Ângela I.5100, IPA-05 and IPA-06, are indicated to plant breeding programs.

  13. Kristalisasi Likopen Dari Buah Tomat (Lycopersicon esculentum) Menggunakan Antisolvent

    OpenAIRE

    Sinaga, Deviana Christianty Stefania

    2015-01-01

    Lycopene is a carotenoid compounds which as an antioxidants and has a red pigment found in plant tissues such as tomatoes. Lycopene could be made into potential compound to health of human body through processed tomato products. Studies on the utilization of lycopene had been conducted to determine the effect of comparison feed and solvent (F/S) and the addition of methanol for extraction of lycopene from tomatoes using mixture solvent of hexane:ethyl acetate (1:1) followed ...

  14. Somatic hybridization between Lycopersicon esculentum and Solanum tuberosum

    NARCIS (Netherlands)

    Schoenmakers, H.C.H.

    1993-01-01

    This thesis describes an analysis of the possibilities and limitations of asymmetric somatic hybridization between tomato and potato. For this, nitrate reductase-deficient tomato mutants were isolated and characterized. It was shown that two of these mutations could be used as selectable

  15. MENINGKATKAN PERTUMBUHAN TANAMAN TOMAT (Lycopersicon esculentum DENGAN SISTEM BUDIDAYA HIDROPONIK

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    Catur Wasonowati

    2011-03-01

    Full Text Available Tomato is vegetable that has high economic and nutritional value. Hydroponics is the cultivation of plants without soil but using rice husk charcoal, rockwool, pumice, sand, gravel, perlite water and air media. This research aims to study the effect of nutrients and polybag size on growth and yield of hydroponic tomatoes. The experiment was conducted in Kassa House of Agroekoteknologi Departement Faculty of Agriculture Trunojoyo University. Fertilizers used were Hidrogroup and Greentonik while polybag size are 30x30 cm, 30x40 cm and 40x40 cm. The design of this study using Complete Randomized Design (CRD with 3 replications. The result showed that this research affect to the vegetative phase. No interaction between type of nutrition and polybag size on plant height, leaf number, stem diameter, number of flowers, flowering time of tomato. Type of nutrition significantly affect plant height, leaf number, stem diameter, flower number and wet and dry weight of stems and leaves, whereas the size of polybags significantly affects in the number of leaves, wet and dry weight of stems and leaves of tomato.

  16. Regulation of tomato (Lycopersicon esculentum Mill.) fruit setting ...

    African Journals Online (AJOL)

    Dandena

    2012-06-26

    Jun 26, 2012 ... In general, the study indicated that 2, 4-D is important in tomato ..... Research Experience and Production. Prospects. Research Report 43. Ethiopian Agricultural Research. Organization Addis Ababa, Ethiopia. p. 48.

  17. The incorporation gene of tomato fruit firmness (Lycopersicon esculentum Mill.

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    Zdravković Jasmina

    2009-01-01

    Full Text Available Tomato fruit firmness is a polygenetic trait and depends on firmness components pericarp thickness, firmness of epidermis and firmness of flash. The accumulation of favourable traits ratio for each component (towards the increase of expression the fruit firmness can be increased. This paper deals with aspects of increasing fruit firmness by increasing firmness of epidermis and thickness of pericarp. By using genotypes with rin (ripening inhibitor gene, we were able to accomplish great firmness of fruits, especially firmness of flash. The expression of these traits cause the asynchronization of maturing process so the fruits do not over mature or soften. Genetic effects have been evaluated by researching the average values of fruit firmness in six diallel parent lines (D-150, S-49, S-35, H-52, Kg-z and SP-109 and progeny (P1, P2, F1, F2, BC1 and BC2 by applying additive dominant model with three and six parameters (Mather and Jinks, 1982. Mean values of fruit firmness for parents and progeny were significantly different. Firmness of fruits is a trait influenced first of all by additive gene since they were found in all researched combinations. Epystatic gene effect was important in inheriting process for all three two-gene interactions. The stabile duplicate type of epystsase was found, which in this case reduces the unfavourable effects of dominant genes of parents with soft fruits. .

  18. Flow cytometric analysis of oil palm: a preliminary analysis for cultivars and genomic DNA alteration

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    Warawut Chuthammathat

    2005-12-01

    Full Text Available DNA contents of oil palm (Elaeis guineensis Jacq. cultivars were analyzed by flow cytometry using different external reference plant species. Analysis using corn (Zea mays line CE-777 as a reference plant gave the highest DNA content of oil palm (4.72±0.23 pg 2C-1 whereas the DNA content was found to be lower when using soybean (Glycine max cv. Polanka (3.77±0.09 pg 2C-1 or tomato (Lycopersicon esculentum cv. Stupicke (4.25±0.09 pg 2C-1 as a reference. The nuclear DNA contents of Dura (D109, Pisifera (P168 and Tenera (T38 cultivars were 3.46±0.04, 3.24±0.03 and 3.76±0.04 pg 2C-1 nuclei, respectively, using soybean as a reference. One haploid genome of oil palm therefore ranged from 1.56 to 1.81±109 base pairs. DNA contents from one-year-old calli and cell suspension of oil palm were found to be significantly different from those of seedlings. It thus should be noted that genomic DNA alteration occurred in these cultured tissues. We therefore confirm that flow cytometric analysis could verify cultivars, DNA content and genomic DNA alteration of oil palm using soybean as an external reference standard.

  19. Development of Bemisia tabaci (Gennadius,1889 biotype B on Lycopersicon spp. genotypes

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    Marilene Fancelli

    2002-01-01

    Full Text Available Whiteflies are phytophagous insects, whose nymphs and adults suck the phloem sap, causing direct damage due to host plant weakness. In tomato (Lycopersicon spp. crops, they are important vectors of limiting fitoviruses. The objective of this study was to determine the effects of Lycopersicon spp. genotypes on Bemisia tabaci (Gennadius, 1889 biotype B development under greenhouse conditions. The evaluated genotypes were LA462 (L. peruvianum, LA716 (L. pennellii, LA1584 (L. pimpinellifolium, LA1609 (L. peruvianum, LA1739 (L. hirsutum, P25 (L. esculentum, PI134417 (L. hirsutum f. glabratum and Santa Clara (L. esculentum. LA716 was non-preferred for oviposition by the whitefly, which suggests an antixenotic effect. LA1584 showed an antibiotic resistance because nymphal survival was reduced and nymphal developmental time was increased. Antixenotic resistance was observed in LA1739 and PI134417, based on a reduction of oviposition. PI134417 also reduced nymphal survival, which suggests an antibiotic effect, but LA1739 was suitable for insect development. LA1609 was highly preferred for oviposition, however it reduced insect survival. P25 and Santa Clara (L. esculentum were highly preferred for oviposition.

  20. Effects of the Lycopersicon chmielewskii sucrose accumulator gene (sucr) on fruit yield and quality parameters following introgression into tomato.

    Science.gov (United States)

    Chetelat, R T; Deverna, J W; Bennett, A B

    1995-07-01

    A gene controlling fruit sucrose accumulation, sucr, was introgressed from the wild tomato species Lycopersicon chmielewskii into the genetic background of a hexose-accumulating cultivated tomato, L. esculentum. During introgression, the size of the L. chmielewskii chromosomal segment containing sucr was reduced by selection for recombination between RFLP markers for the sucr gene and flanking loci. The effects of sucr on soluble solids content, fruit size, yield and other fruit parameters were studied in the genetic background of the processing tomato cultivar 'Huntl00'. In a segregating BC5F2 generation, the smallest introgression containing sucr-associated markers was necessary and sufficient to confer high-level sucrose accumulation, the effects of which were completely recessive. Fruit of sucr/sucr genotypes were smaller than those of +/sucr or +/+ genotypes at all stages of development. The timing of sugar accumulation and total sugar concentration were unaffected by sugar composition. No differences in total fruit biomass (fresh weight of red and green fruit) at harvest were observed between the genotypes, and sucrose accumulators produced greater numbers of fruit than hexose accumulators in one family. However, the proportion of ripe fruit at harvest, and hence yield of ripe fruit, as well as average ripe fruit weight and seed set were reduced in sucr/sucr genotypes. Sucrose accumulation was also associated with increased soluble solids content, consistency, serum viscosity, predicted paste yield and acidity, and decreased color rating. In the first backcross to L. chmielewskii, hexose accumulators (+/sucr) had larger fruit than sucrose accumulators (sucr/sucr), while no difference in soluble solids was detected.

  1. Cell wall glycosidase activities and protein content variations during fruit development and ripening in three texture contrasted tomato cultivars

    Science.gov (United States)

    Konozy, Emadeldin H.E.; Causse, Mathilde; Faurobert, Mireille

    2012-01-01

    Excessive softening is the main factor limiting fruit shelf life and storage. It is generally acceptable now that softening of fruit which occurs during the ripening is due to synergistic actions of several enzymes on cell wall polysaccharides. As a subject for this study, we have assayed some glycosidase activities using three tomato species (Lycopersicon esculentum) contrasted for their texture phenotypes; the cherry tomato line Cervil (Solanum lycopersicum var. cerasiforme), a common taste tomato line Levovil (S. lycopersicum Mill.) and VilB a modern line, large, firmer and with good storage capability. Four glycosidase activities namely α-galactosidase, β-galactosidase, β-mannosidase and β-glucosidase were extracted from tomato’s cell wall of the three species. Cell wall protein from fruits pericarp was extracted and compared among the three cultivars at the following stages; 14 days post anthesis (14DPA) fruit; 21 days post anthesis (21DPA), turning (breaker), red and over ripe. When glycolytic activities were also compared among these cultivars at the precited development stages, gross variations were noticed from stage to stage and also from species to species in accordance with the fruit firmness status. Interestingly, VilB cultivar, the firmer among the other two, though possessed the highest total protein content, exhibited the lowest enzymatic activities. Taken together, these results may therefore allow us to conclude that studies of glycolytic activities in a single tomato cultivar cannot be generalized to all species. On the other hand, relating fruit development to glycosidase activities should logically be coupled to these enzymes from cell wall compartment. PMID:23961187

  2. Influence of the Host Cultivar on Disease and Viral Accumulation Dynamics in Tomato under Mixed Infection with Potato virus X and Tomato mosaic virus

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    O.S. Balogun

    2005-04-01

    Full Text Available The primary leaves of seedlings of tomato (Lycopersicon esculentum Mill. cultivar Fukuju No. 2 (a common Japanese cultivar that is susceptible to Tobacco mosaic virus (TMV, genus Tobamovirus were inoculated at the five-true leaf stage with the O strain of Potato virus X (PVX, genus Potexvirus and with a mixture of that strain plus Tomato mosaic virus (ToMV, genus Tobamovirus. Inoculation resulted in varying degrees of disease manifestation. During the acute stage of the resulting severe disease (between 5 and 12 days postinoculation, PVX and ToMV levels rose considerably in both the inoculated and the systematically infected leaves. Furthermore, levels of PVX in the systemically infected upper leaves (positions 5 to 7 of plants with a mixed infection were three to six times as high as in plants given the single infection, as determined by direct double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA. In tomato cv. GCR 236 (+/+, symptom manifestation and the accumulation of both PVX and ToMV closely followed the pattern recorded for cv. Fukuju No. 2. In cv. GCR 237 (Tm-1 plants, however, only PVX accumulated while ToMV whether inoculated singly or mixed with PVX was detected neither in the inoculated nor in the systemically infected leaves even 14 days after inoculation. In contrast to other cultivars, SDS-PAGE, Western blot and Northern blot hybridization did not reveal any enhancement of the coat protein and genomic RNA of PVX in such systemically infected leaves. Consequently, the characteristic severe symptoms normally associated with mixed infection in TMV-susceptible cultivars were absent.

  3. Mechanisms and genetic control of interspecific crossing barriers in Lycopersicon. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Mutschler, M.A.

    1997-04-30

    Deficiency of Lycopersicon esculentum allele (E) was observed from the RFLP and isozyme data of the F{sub 2} populations derived from the cross L. esculentum x L. pennellii. The genome composition of the F{sub 2} populations containing L. pennellii cytoplasm (F{sub 2}{sup Lp4}) has a lower proportion of the homozygous L. pennellii (PP) genotypes and a higher proportion of heterozygote (EP) genotypes than that of the F{sub 2} populations containing L. esculentum cytoplasm (F{sub 2}{sup Le}). A lower proportion of the L. pennellii alleles (P) was also observed in F{sub 2}{sup Lp4} as compared to F{sub 2}{sup Le} when each marker locus was tested individually. To study the effects of gametic and zygotic selection on segregation distortion, the expected patterns of segregation at a marker locus were derived for ten selection models with gametic or zygotic selection at a hidden linked locus. Segregation distortion caused by four of the selection models studied can be uniquely identified by the patterns of significance expected for the likelihood ratio tests at the marker loci. Comparison of the chromosomal regions associated with specific selection models across populations (of this experiment and previous publications) indicated that the segregation distortion observed in chromosome 10 is associated with zygotic selection affecting both arms of the chromosome, and cytoplasm substitution has the effect of decreasing the segregation distortion on the long arm of the chromosome.

  4. Mechanisms and genetic control of interspecific crossing barriers in Lycopersicon. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Mutschler, M.A.

    1997-04-30

    Deficiency of Lycopersicon esculentum allele (E) was observed from the RFLP and isozyme data of the F{sub 2} populations derived from the cross L. esculentum x L. pennellii. The genome composition of the F{sub 2} populations containing L. pennellii cytoplasm (F{sub 2}{sup Lp4}) has a lower proportion of the homozygous L. pennellii (PP) genotypes and a higher proportion of heterozygote (EP) genotypes than that of the F{sub 2} populations containing L. esculentum cytoplasm (F{sub 2}{sup Le}). A lower proportion of the L. pennellii alleles (P) was also observed in F{sub 2}{sup Lp4} as compared to F{sub 2}{sup Le} when each marker locus was tested individually. To study the effects of gametic and zygotic selection on segregation distortion, the expected patterns of segregation at a marker locus were derived for ten selection models with gametic or zygotic selection at a hidden linked locus. Segregation distortion caused by four of the selection models studied can be uniquely identified by the patterns of significance expected for the likelihood ratio tests at the marker loci. Comparison of the chromosomal regions associated with specific selection models across populations (of this experiment and previous publications) indicated that the segregation distortion observed in chromosome 10 is associated with zygotic selection affecting both arms of the chromosome, and cytoplasm substitution has the effect of decreasing the segregation distortion on the long arm of the chromosome.

  5. Caracterización y evaluación de recursos genéticos del genero Lycopersicon

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    Vallejo Cabrera Franco Alirio

    1993-12-01

    Full Text Available

    Mediante los descriptores de IBPGR se caracterizaron y evaluaron 118 introducciones del género Lycopersicon, en el Centro Experimental de la Universidad Nacional Sede Palmira. Se utilizó el diseño experimental de Bloques al azar con dos repeticiones; la parcela experimental estuvo constituida por seis plantas. Se estudiaron nueve caracteres relacionados con la planta, diez caracteres relacionados con el fruto, componentes del rendimiento y presencia o ausencia de plagas y enfermedades. Se encontró gran variabilidad para la mayoría de los caracteres. Las variedades de la especie cultivada, L. esculentum, presentaron las mayores producciones por planta y el mayor tamaño de fruto. Las especies silvestres; presentaron mayor resistencia a plagas y enfermedades. Se destacaron los siguientes variedades de L. esculentum por presentar altas producciones y tolerancia a plagas y enfermedades: ADB 18, ADB 400, 1600, 1601 Y Rio Fuego.

    118 accessions of the genus Lycopersicon were characterized and evaluated to determinate the genetic potential, using the IBPGR descriptors. The experiment was conducted, using a complete random design and two repetitions. Characteristics concerned to the plant, fruit, yield components and resistence to insects and disease was studied. Important fenotipic differences were founded. The varieties of the cultivated specie L. esculentum, showed heigh productions per plant and heigh average fruit weight. The wild accessions showed heigh resistence to the insects and pathogens. ADBI8, ADB 400, 1600, 1601 and Rio Fuego accessions of the L. esculentum showd heigh productions per plant and heigh resistence to the insects and pathogens.

  6. EFECTO DE LA SALINIDAD EN LA CONDUCTIVIDAD HIDRÁULICA DE LAS RAÍCES Y LAS RELACIONES HÍDRICAS EN HOJAS DE DOS ESPECIES DE TOMATE (L. esculentum Y L. chessmanii)

    OpenAIRE

    D. Morales; Rodríguez, P; J. M. Dell'Amico; María de J. Sánchez- Blanco; A. Torrecillas

    2003-01-01

    El presente trabajo se realizó con el objetivo de estudiar el comportamiento de la conductividad hidráulica de las raíces y las relaciones hídricas en hojas de dos especies de tomate (Lycopersicon esculentum var. Amalia y Lycopersicon cheesmanii) sometidas por períodos cortos a diferentes tratamientos con NaCl en el medio. Las plantas se desarrollaron en macetas con arena sílice introducidas en bandejas en las que se adicionó solución nutritiva de Hoagland. A un grupo de cuatro plantas por tr...

  7. PENDEKATAN MULTIVARIAT UNTUK PENGUKURAN KUALITAS TOMAT (Lycopersicon esculentum BERDASARKAN PARAMETER WARNA Multivariate Approach to The Measurement of Tomato (Lycopersicon esculentum Quality Based on Color Parameters

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    Rudiati Evi Masithoh

    2012-05-01

    Full Text Available In this study, multivariate linear regression (MLR was used to predict the content of Brix, total carotene, citric acid,and vitamin C of tomato based on RGB color parameters. Tomatoes were stored at 6 °C and 28 °C then their quality parameters were measured. R, G, and B values were measured non-destructively using computer vision system developed in the previous study. Brix, total carotene, citric acid, and vitamin C were determined by conventional procedures in the laboratory. Data analysis showed that the MLR calibration models could be used to predict Brix, total carotene, citric acid, and vitamin C with R2  of  0.77and 0.72, 0.902 and 0.85, 0.71 and 0.77, as well as 0.88 and 0.82 for temperature of 6 °C and 28 °C, respectively. ABSTRAK Pada penelitian ini, multivariate linier regression (MLR digunakan untuk memprediksi kandungan Brix, karoten total,asam sitrat, dan vitamin C dari tomat berdasarkan parameter warna RGB. Tomat disimpan pada suhu 6 °C dan 28 °C kemudian diukur parameter kualitasnya. Nilai R, G, dan B diukur secara non-destructive dari computer vision system yang dikembangkan pada penelitian sebelumnya. Parameter kualitas Brix, karoten total, asam sitrat, dan vitamin C ditentukan secara destructive dengan prosedur konvensional di laboratorium. Analisis data menunjukkan bahwa model kalibrasi MLR dapat digunakan untuk memprediksi Brix, karoten total, asam sitrat, dan vitamin C dengan R2 sebesar0,77dan 0,72, 0,902 dan 0,85, 0,71 dan 0,77, serta 0,88 dan 0,82 untuk suhu 6 °C dan 28 °C secara berturutan.

  8. Microorganismos benéficos como biofertilizantes eficientes para el cultivo del tomate (Lycopersicon esculentum, Mill Beneficial microorganisms as efficient biofertilisers for tomato crops (Lycopersicon esculentum, Mill

    Directory of Open Access Journals (Sweden)

    Hernández Annia

    2005-12-01

    Full Text Available En el uso y manejo de biofertilizantes en la agricultura, uno de los principales problemas es el desconocimiento de las especies presentes en los agroecosistemas y en la rizosfera de los cultivos, para su posible utilización eficiente. Desde el punto de vista ecológico, es importante conocer los integrantes de la comunidad bacteriana que favorecen su aplicación como inoculantes y propician un efecto agrobiológico positivo en los cultivos agrícolas. Esta investigación se desarrolló con el objetivo de evaluar la efectividad agrobiológica de Azospirillum sp, en el crecimiento, desarrollo y rendimiento en el cultivo del tomate. Para ello, se partió de seleccionar el género microbiano predominante en la rizosfera del cultivo y posteriormente se evaluó el efecto de su inoculación a partir de la respuesta del cultivo. Los resultados demostraron que los géneros Pseudomonas, Azospirillum, Azotobacter, Bacillus y Streptomyces, forman parte de la comunidad microbiana de la rizosfera del tomate, en las condiciones estudiadas, y que Azospirillum es el género dominante. La inoculación artificial de esta rizobacteria causó un efecto positivo sobre el crecimiento de las plántulas, así como en el estado nutricional de las plantas, con un rendimiento agrícola superior a un 11 % con respecto a las plantas testigo. Se obtuvo un alto nivel poblacional en la rizosfera de las plantas inoculadas. Palabras clave: rizosfera, inoculante, crecimiento, rendimiento.One of the main problems regarding the efficient use and management of biofertilizers in agriculture lies in the unknown species present in agro-ecosystems and crop rhizospheres. From the ecological point of view, it is important to know the members of the bacterial population allowing them to be applied as inoculants and enable a positive agro-biological effect on agricultural crops. This investigation was aimed at evaluating the agro-biological effectiveness of Azospirillum sp. on tomato growth, development and yield. The predominating microbial genus in the crop rhizosphere was thus selected and the effect of inoculating it was then evaluated, judging by the crop's response. Results showed that Pseudomonas, Azospirillum, Azotobacter, Bacillus and Streptomyces belonged to the microbial population of the tomato rhizosphere under the studied conditions, Azospirillum being the most prominent genus. Artificial inoculation of this rhizobacteria caused a positive effect on seedling growth as well as on plant nutritional stage, the agricultural yield being higher in 11% compared to control plants. A high microbial population level was recorded in the rhizosphere of the inoculated plants. Key words: rhizosphere, inoculant, growth, yield.

  9. Trace elements and toxic metals in intensively produced tomatoes (lycopersicon esculentum Metales traza y metales tóxicos en tomates producidos intensivamente (lycopersicon esculentum

    Directory of Open Access Journals (Sweden)

    G. Luis

    2012-10-01

    Full Text Available Introduction: Tomato is considered a healthy food due to its high content in lycopene and other health-promoting natural compounds. Tomatoes have, undoubtedly, assumed the status of a food with functional properties, considering the epidemiological evidence of reducing the risk of certain types of cancers. Objective: Samples of tomatoes from Morocco were analyzed for the mineral composition. Methods: 94 tomato samples from Morocco were analyzed. Flame Atomic Absorption Spectrophotometry (FAAS was used to determine essential elements (Copper (Cu, iron (Fe, manganese (Mn and zinc (Zn and Atomic Absorption Spectrophotometry with Graphite Furnace (GAAS was used to analyzed cadmium (Cd and lead (Pb. Results: The mean concentrations were 0.17 mg/kg, 0.73 mg/kg, 0.20 mg/kg, 0.44 mg/kg, 7.58 μg/kg and 15.8 μg/kg for Cu, Fe, Mn, Zn, Cd and Pb, respectively. The highest contribution to the intakes was observed for Cu (0.67% for adults while that Zn showed the lowest contribution (0.14% for adult males and 0.20% for adult females. Conclusions: Tomatoes are a good source of essential elements for the diet, mainly iron and zinc. Tomatoes consumption does not significantly affect the intake of heavy metals.Introducción: El tomate es considerado un alimento saludable debido a su alto contenido en licopeno y otros componentes naturales promotores de salud. Indudablemente, los tomates han asumido el estatus de un alimento con propiedades funcionales, considerando desde un punto de vista epidemiológico reducir ciertos tipos de cánceres Objetivo: Analizar la composición mineral de muestras de tomates de Marruecos. Métodos: 94 muestras de tomates de Marruecos fueron analizadas. Espectrofotometría de Absorción Atómica por llama (FAAS se usó para determinar elementos esenciales (Cobre (Cu, hierro (Fe, manganeso (Mn y zinc (Zn y Espectrofotometría de Absorción Atómica con cámara de grafito (GAAS se usó para analizar cadmio (Cd y plomo (Pb. Resultados: Las concentraciones medias fueron 0,17 mg/kg, 0,73 mg/kg, 0,20 mg/kg, 0,44 mg/kg, 7,58 μg/kg and 15,8 μg/kg para Cu, Fe, Mn, Zn, Cd y Pb, respectivamente. La contribución a la ingesta más alta fue observada para el Cu (0,67% para adultos mientras que el Zn presentó la contribución más baja (0,14% para hombres adultos y 0,20% para mujeres adultas. Conclusión: Los tomates son una buena fuente de elementos esenciales para la dieta, principalmente hierro y zinc. El consumo de tomates no afecta significativamente a la ingesta de metales pesados.

  10. Genes from Lycopersicon chmielewskii affecting tomato quality during fruit ripening.

    Science.gov (United States)

    Azanza, F; Kim, D; Tanksley, S D; Juvik, J A

    1995-08-01

    Three chromosomal segments from the wild tomato, L. chmielewskii, introgressed into the L. esculentum genome have been previously mapped to the middle and terminal regions of chromosome 7 (7M, 7T respectively), and to the terminal region of chromosome 10 (10T). The present study was designed to investigate the physiological mechanisms controlled by the 7M and 7T segments on tomato soluble solids (SS) and pH, and their genetic regulation during fruit development. The effects of 7M and 7T were studied in 64 BC2F5 backcross inbred lines (BILs) developed from a cross between LA 1501 (an L. esculentum line containing the 7M and 7T fragments from L. chmielewskii), and VF145B-7879 (a processing cultivar). BILs were classified into four homozygous genotypes with respect to the introgressed segments based on RFLP analysis, and evaluated for fruit chemical characteristics at different harvest stages. Gene(s) in the 7M fragment reduce fruit water uptake during ripening increasing pH, sugars, and SS concentration. Gene(s) in the 7T fragment were found to be associated with higher mature green fruit starch concentration and red ripe fruit weight. Comparisons between tomatoes ripened on or off the vine suggest that the physiological mechanisms influenced by the L. chmielewskii alleles are dependent on the translocation of photosynthates and water during fruit ripening.

  11. Natural variation in the Pto pathogen resistance gene within species of wild tomato (Lycopersicon). I. Functional analysis of Pto alleles.

    Science.gov (United States)

    Rose, Laura E; Langley, Charles H; Bernal, Adriana J; Michelmore, Richard W

    2005-09-01

    Disease resistance to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) in the cultivated tomato, Lycopersicon esculentum, and the closely related L. pimpinellifolium is triggered by the physical interaction between plant disease resistance protein, Pto, and the pathogen avirulence protein, AvrPto. To investigate the extent to which variation in the Pto gene is responsible for naturally occurring variation in resistance to Pst, we determined the resistance phenotype of 51 accessions from seven species of Lycopersicon to isogenic strains of Pst differing in the presence of avrPto. One-third of the plants displayed resistance specifically when the pathogen expressed AvrPto, consistent with a gene-for-gene interaction. To test whether this resistance in these species was conferred specifically by the Pto gene, alleles of Pto were amplified and sequenced from 49 individuals and a subset (16) of these alleles was tested in planta using Agrobacterium-mediated transient assays. Eleven alleles conferred a hypersensitive resistance response (HR) in the presence of AvrPto, while 5 did not. Ten amino acid substitutions associated with the absence of AvrPto recognition and HR were identified, none of which had been identified in previous structure-function studies. Additionally, 3 alleles encoding putative pseudogenes of Pto were isolated from two species of Lycopersicon. Therefore, a large proportion, but not all, of the natural variation in the reaction to strains of Pst expressing AvrPto can be attributed to sequence variation in the Pto gene.

  12. Natural variation in the Pto disease resistance gene within species of wild tomato (Lycopersicon). II. Population genetics of Pto.

    Science.gov (United States)

    Rose, Laura E; Michelmore, Richard W; Langley, Charles H

    2007-03-01

    Disease resistance to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) in the host species Lycopersicon esculentum, the cultivated tomato, and the closely related L. pimpinellifolium is triggered by the physical interaction between the protein products of the host resistance (R) gene Pto and the pathogen avirulence genes AvrPto and AvrPtoB. Sequence variation at the Pto locus was surveyed in natural populations of seven species of Lycopersicon to test hypotheses of host-parasite coevolution and functional adaptation of the Pto gene. Pto shows significantly higher nonsynonymous polymorphism than 14 other non-R-gene loci in the same samples of Lycopersicon species, while showing no difference in synonymous polymorphism, suggesting that the maintenance of amino acid polymorphism at this locus is mediated by pathogen selection. Also, a larger proportion of ancestral variation is maintained at Pto as compared to these non-R-gene loci. The frequency spectrum of amino acid polymorphisms known to negatively affect Pto function is skewed toward low frequency compared to amino acid polymorphisms that do not affect function or silent polymorphisms. Therefore, the evolution of Pto appears to be influenced by a mixture of both purifying and balancing selection.

  13. Capítulo III: caracterización y evaluación de recursos genéticos del género Lycopersicon

    Directory of Open Access Journals (Sweden)

    Sierra Tulio Alberto

    1994-12-01

    Full Text Available

    Con el fin de determinar el potencial genético de 118 introducciones del género Lycopersicon se realizó la caracterización y evaluación, en el Centro Experimental de la Universidad Nacional Sede Palmira, utilizando los descriptores recomendados por el IBPGR (1981. Se utilizó el diseño experimental de Bloques al azar con dos repeticiones. La parcela experimental estuvo constituida por seis plantas. Se estudiaron nueve caracteres relacionados con la planta, diez caracteres relacionados con el fruto, componentes del rendimiento y  presencia o ausencia de plagas y enfermedades. Se encontró una gran variabilidad para la mayoría de los caracteres estudiados. Las variedades de la especie cultivada, L. esculentum, presentaron las mayores producciones por planta y el mayor tamaño de fruto, en comparación con las especies silvestres; sin embargo, estas últimas presentaron mayor resistencia a plagas y enfermedades. Se destacaron los siguientes variedades de L. esculentum por presentar altas producciones y tolerancia a plagas y enfermedades: ADB 18, ADB 400, 1600, 1604  y Río Fuego.

    118 accessions of the genus Lycopersicon were characterized and evaluated to determinate the genetic potential, using the IBPGR descriptors. The experiment was conducted, using a complete random design and two repetitions. Characteristics concerned to the plant, fruit yield components and resistence to insects and disease was studied. Important fenotipic differences, for the evaluated characters, were founded. The varieties of the cultivated specie L. esculentum, showed heigh productions per plant and heigh average fruit weight. The wild accessions showed heigh resistence to the insects and pathogens. ADB18,  ADB 400, 1600, 1601 and Rio Fuego accessions of the L. esculentum  showd heigh productions per plant and heigh resistence to the insects and pathogens.

  14. Identification of conserved microRNAs and their target genes in tomato (Lycopersicon esculentum).

    Science.gov (United States)

    Yin, Zujun; Li, Chunhe; Han, Xiulan; Shen, Fafu

    2008-05-15

    MicroRNAs (miRNAs) are a class of non-coding RNAs that have important gene regulation roles in various organisms. To date, a total of 1279 plant miRNAs have been deposited in the miRNA miRBase database (Release 10.1). Many of them are conserved during the evolution of land plants suggesting that the well-conserved miRNAs may also retain homologous target interactions. Recently, little is known about the experimental or computational identification of conserved miRNAs and their target genes in tomato. Here, using a computational homology search approach, 21 conserved miRNAs were detected in the Expressed Sequence Tags (EST) and Genomic Survey Sequence (GSS) databases. Following this, 57 potential target genes were predicted by searching the mRNA database. Most of the target mRNAs appeared to be involved in plant growth and development. Our findings verified that the well-conserved tomato miRNAs have retained homologous target interactions amongst divergent plant species. Some miRNAs express diverse combinations in different cell types and have been shown to regulate cell-specific target genes coordinately. We believe that the targeting propensity for genes in different biological processes can be explained largely by their protein connectivity.

  15. Quality comparison of hydroponic tomatoes (Lycopersicon esculentum) ripened on and off vine

    Science.gov (United States)

    Arias, R.; Lee, T. C.; Specca, D.; Janes, H.

    2000-01-01

    There is a general belief that the quality of tomatoes ripened on vine is better than tomatoes ripened off the vine, influencing among other parameters, the price of this commodity. We compared the quality of hydroponic tomatoes ripened on and off vine by chemical, physical, and sensory evaluation to find what attributes are affected and to what extent. Lycopene, beta-carotene, total and soluble solids, moisture content, ascorbic acid, acidity, pH, texture, and color were analyzed. Tomatoes ripened on vine had significantly more lycopene, beta-carotene, soluble and total solids, higher a* and lower L*, and were firmer. However, a 100-judge panel rated only the color and overall liking of the vine-ripened tomatoes as more intense than the fruit ripened off vine. Therefore, the chemical and physical differences were mostly not large enough to influence the panelist's perception. The characterization of tomatoes ripened on and off vine may help to guide post-harvest handling and treatment and to improve the quality of tomatoes ripened off vine.

  16. Characterization and content of flavonoid glycosides in genetically modified tomato (Lycopersicon esculentum) fruits.

    Science.gov (United States)

    Le Gall, Gwénaëlle; DuPont, M Susan; Mellon, Fred A; Davis, Adrienne L; Collins, Geoff J; Verhoeyen, Martine E; Colquhoun, Ian J

    2003-04-23

    There is a growing interest in producing food plants with increased amounts of flavonoids because of their potential health benefits. Tomatoes contain small amounts of flavonoids, most of which are located in the peel of the fruit. It has been shown that flavonoid accumulation in tomato flesh, and hence an overall increase in flavonoid levels in tomato fruit, can be achieved by means of simultaneous overexpression of the maize transcription factors LC and C1. Fruit from progeny of two modified lines (2027 and 2059) was selected for a detailed analysis and individual identification of flavonoids, at different stages of maturity. Nine major flavonoids were detected in the flesh of transgenic ripe tomatoes. LC/NMR, LC/MS, and LC/MS/MS enabled us to identify these as kaempferol-3,7-di-O-glucoside (1), kaempferol-3-O-rutinoside-7-O-glucoside (2), two dihydrokaempferol-O-hexosides (3 and 4), rutin (5), kaempferol-3-O-rutinoside (6), kaempferol-3-O-glucoside (7), naringenin-7-O-glucoside (8) and naringenin chalcone (9), which were quantified by HPLC/DAD. All but 5, 6, and 9 were detected in tomato for the first time. The total flavonoid glycoside content of ripe transgenic tomatoes of line 2059 was about 10-fold higher than that of the controls, and kaempferol glycosides accounted for 60% of this. Kaempferol glycosides comprised around 5% of the flavonoid glycoside content of ripe control tomatoes (the rest was rutin and naringenin chalcone). The rutin concentration in both transgenic and control fruits was similar.

  17. Characterization of an ethylene-related small multigene family from Lycopersicon esculentum

    Energy Technology Data Exchange (ETDEWEB)

    Holdsworth, M.

    1987-01-01

    cDNA clones derived from a tomato ripening-related cDNA library were used in RNA dot-blot experiments to investigate changes in the abundance of ripening related mRNAs during both natural and ethylene-induced ripening. Accumulation of the ripening-related mRNAs during natural ripening began at the time of autocatalytic ethylene production by the fruit, reached a maximum in orange fruit and declined as they became red. Analysis of the induction kinetics of these mRNAs revealed several patterns of expression as tomatoes ripened. The pTOM 13 cDNA insert was sequenced and used to identify related sequences in a tomato genomic library. 21 hybridizing genomic clones were isolated and divided into three groups of similar sequences based on their restriction maps. The DNA sequences of two of these groups of genomic clones that hybridized to pTOM 13 were determined. This allowed the identification of an incomplete pTOM 13-homologous gene, and a closely related complete gene. Nuclei were isolated from unwounded and wounded leaves and were used in run-off transcription experiments in the presence of (..cap alpha../sup 32/P)UTP. (/sup 32/P)-labelled RNA obtained from transcription experiments was used in dot-blot experiments against pTOM 13 and related genomic subclones. The results of these experiments demonstrated that the accumulation of pTOM 13-related genes in leaves may be controlled at transcriptional and post-transcriptional levels.

  18. Quality perception of organically grown tomatoes (Lycopersicon esculentum Mill. in Vienna, Austria

    Directory of Open Access Journals (Sweden)

    PK Ng’ang’a

    2015-12-01

    Full Text Available Austria is one of the major organic tomato producing countries for local and export marketing. These tomatoes are produced in parts of Austria especially around Vienna where their production system has to meet stringent organic quality standards in both local and international markets. These quality standards may put considerable strain on farmers and are normally formulated without famers’ participation so may not be wholly representative of the farmers’ quality interpretation. The aim of this paper is therefore to determine the Austrian organic tomatoes growers’ perception and practice of quality and challenges. Qualitative semi-structured interviews were carried out among 28 organic tomatoes farmers in Vienna, Austria. Findings suggest that quality of organic tomatoes is mainly perceived in terms of both informal values (big fruit size, long shelf life, food security and amount of income received from tomato sales as well as formal norms (non- application of chemicals, human health, damage free, sweet taste, red colour, and juiciness. There were no gendered differences in quality perception among the growers. High costs of production inputs were identified as the main challenge to attaining quality in organic tomatoes. Following these findings, there is need for effective participation of growers in formulation of standards as well as subsidizing of production inputs by the government. The Austrian tomato growers as well as local and international retailers should work closely to increase the price received by the Austrian organic tomato growers so that it more adequately covers their production costs.

  19. The effect of water stress and salinity on growth and physiology of tomato (Lycopersicon esculentum Mil.

    Directory of Open Access Journals (Sweden)

    Giannakoula Anastasia E.

    2013-01-01

    Full Text Available Carotenoids like lycopene are important pigments found in photosynthetic pigment-protein complexes in plants. They are responsible for the bright colors of fruits and vegetables and perform various functions in photosynthesis. Our research has shown that the application of moderate salt stress on tomato plants can enhance lycopene and potentially other antioxidant concentrations in fruits. The increase in lycopene in response to salt stress in the tomato fruits varied from 20% to 80%. Although the specific biological mechanisms involved in increasing fruit lycopene deposition has not been clearly elucidated, evidence suggests that increasing antioxidant concentrations is a primary physiological response of the plant to salt stress. Additionally drought stress during cultivation increased the antioxidant capacity of tomato fruit while maintaining the lycopene concentration. In addition, the effects of silicium were investigated, added to the nutrient solution either at low concentration or at an increased concentration. The present study clearly indicates that an enhanced silicium supply to tomato increases markedly the lycopene contents, irrespective of the salinity status in the tomato fruit.

  20. Evaluación de densidades de siembra en tomate (Lycopersicon esculentum Mill en invernadero

    Directory of Open Access Journals (Sweden)

    Jos\\u00E9 Cruz Carrillo

    2003-01-01

    Full Text Available Esta investigación se realizó de junio a diciembre del 2001 en el módulo de horticultura protegida del Instituto Tecnológico Agropecuario de Oaxaca No. 23 ubicado en la ex Hacienda de Nazareno, Xoxocotlan, Oax., con el propósito de evaluar el efecto de tres densidades de plantación en el desarrollo vegetativo y producci ón de diferentes híbridos de tomate con crecimiento indeterminado cultivados bajo invernadero. Los híbridos de tomate fueron el SXT, DRW3410 y SXT0289. No hubo diferencia estad ística para los híbridos, sólo para densidades de plantación, obteniendo los mejores rendimientos 5,3 plantas/m2 y 4 plantas/ m2 con 17,52 y 17,37 kg/m2, respectivamente, lo que represent ó en promedio un rendimiento de 151,6 t/ha.

  1. Quality comparison of hydroponic tomatoes (Lycopersicon esculentum) ripened on and off vine

    Science.gov (United States)

    Arias, R.; Lee, T. C.; Specca, D.; Janes, H.

    2000-01-01

    There is a general belief that the quality of tomatoes ripened on vine is better than tomatoes ripened off the vine, influencing among other parameters, the price of this commodity. We compared the quality of hydroponic tomatoes ripened on and off vine by chemical, physical, and sensory evaluation to find what attributes are affected and to what extent. Lycopene, beta-carotene, total and soluble solids, moisture content, ascorbic acid, acidity, pH, texture, and color were analyzed. Tomatoes ripened on vine had significantly more lycopene, beta-carotene, soluble and total solids, higher a* and lower L*, and were firmer. However, a 100-judge panel rated only the color and overall liking of the vine-ripened tomatoes as more intense than the fruit ripened off vine. Therefore, the chemical and physical differences were mostly not large enough to influence the panelist's perception. The characterization of tomatoes ripened on and off vine may help to guide post-harvest handling and treatment and to improve the quality of tomatoes ripened off vine.

  2. Superoxide dismutase: A possible protective agent against sunscald in tomatoes (lycopersicon esculentum mill.).

    Science.gov (United States)

    Rabinowitch, H D; Sklan, D

    1980-03-01

    Superoxide dismutase (SOD, EC 1.15.1.1) was concentrated from mature-green tomato fruits by gel chromatography. The enzyme was inhibited by cyanide but not by chloroform-ethanol, and appears to contain zinc and lesser amounts of copper. SOD-activity levels were high in immature green fruits, declined to a minimum in the mature-green and breaker stages known to be most susceptible to sunscald damage, increased again until the fruits were pink, and finally decreased through the red-ripe and overripe stages to the level of the mature-green fruit. When tolerance to sunscald damage was induced in mature-green fruits by controlled temperature treatment and samples of the fruits were challenged at various times during this process with a combined heat-and-light treatment known to cause sunscald, SOD activity was found to be inversely related to the susceptibility of the fruit to sunscald damage. It is suggested that superoxide is involved in sunscald injury to tomatoes and that tolerance is acquired through increases in SOD activity. Possibly SOD acts as a general protective agent against photodynamic damage to green tissues in plants that have become conditioned as the result of normal diurnal temperature fluctuations.

  3. Expression of carotenoid biosynthetic pathway genes and changes in carotenoids during ripening in tomato (Lycopersicon esculentum).

    Science.gov (United States)

    Namitha, Kanakapura Krishnamurthy; Archana, Surya Narayana; Negi, Pradeep Singh

    2011-04-01

    To study the expression pattern of carotenoid biosynthetic pathway genes, changes in their expression at different stages of maturity in tomato fruit (cv. Arka Ahuti) were investigated. The genes regulating carotenoid production were quantified by a dot blot method using a DIG (dioxigenin) labelling and detection kit. The results revealed that there was an increase in the levels of upstream genes of the carotenoid biosynthetic pathway such as 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR), 4-hydroxy-3-methyl-but-2-enyl diphosphate reductase (Lyt B), phytoene synthase (PSY), phytoene desaturase (PDS) and ζ-carotene desaturase (ZDS) by 2-4 fold at the breaker stage as compared to leaf. The lycopene and β-carotene content was analyzed by HPLC at different stages of maturity. The lycopene (15.33 ± 0.24 mg per 100 g) and β-carotene (10.37 ± 0.46 mg per 100 g) content were found to be highest at 5 days post-breaker and 10 days post-breaker stage, respectively. The lycopene accumulation pattern also coincided with the color values at different stages of maturity. These studies may provide insight into devising gene-based strategies for enhancing carotenoid accumulation in tomato fruits.

  4. Stability of lycopene in cv. Saladette tomatoes (Lycopersicon esculentum Mill.) stored under different conditions

    OpenAIRE

    R.M. Galicia; R. Verde; E. Ponce; R.O. González; C. Saucedo; Guerrero, I

    2008-01-01

    The objectives of this study were to determine the concentration of lycopene in cv. Saladette tomato subjected to blanching (thermal treatment), to extract the carotenoid and to evaluate the stability of the pigment in solutions added with and without antioxidants, in conditions of darkness at 4, 20 and 60 °C, and in fluorescent light at 20 °C during 30 days. The concentration of lycopene in non blanched tomatoes was 79.20 ug/g, while in blanched tomatoes it was 75.25 ug/g, none presenting an...

  5. Behaviour of the disease resistance gene Asc in protoplasts of Lycopersicon esculentum mill

    NARCIS (Netherlands)

    Moussatos, V.; Witsenboer, H.; Hille, J.; Gilchrist, D.

    1993-01-01

    Action of Asc, a single dominant Mendelian gene controlling disease response at the whole plant level, was detected at the level of individual cells. Protoplasts, freshly isolated from resistant (Asc/Asc) and susceptible (asc/asc) tomato isolines, were differentially sensitive to AAL toxin as observ

  6. BIO-EFFICACY OF INSECTICIDES AGAINST FRUIT BORER (HELICOVERPA ARMIGERA IN TOMATO (LYCOPERSICON ESCULENTUM

    Directory of Open Access Journals (Sweden)

    Ravi Kumar Katroju,

    2014-02-01

    Full Text Available Field experiment carried out during kharif, 2012 at Student’s Farm, College of Agriculture, Rajendranagar, Hyderabad to evaluate the efficacy of insecticides viz., emamectin benzoate 5 SG @11 g a.i. ha-1, emamectin benzoate 5 SG @ 22 g a.i. ha-1, profenophos 50 EC @ 500 g a.i. ha-1, profenophos 50 EC @1000 g a.i. ha-1, spinosad 45 SC @ 100 g a.i. ha-1, bifenthrin 10 EC @ 100 g a.i. ha-1 and Bacillus thuringiensis @ 25 g a.i. ha-1against tomato fruit borer (Helicoverpa armigera. Among all the insecticides, profenophos (1000 g a.i. ha-1 was found to be the most effective one with a maximum reduction in fruit borer population (65.20%, minimum per cent of fruit damage (28.80% and maximum yield (26.43 kg/20 m2 followed by bifenthrin @ 100 g a.i.ha-1 with reduced larval population of 64.51% and damaged fruits 32.60%.

  7. Impact of Drip Irrigation Scheduling on Vegetative Parameters in Tomato (Lycopersicon esculentum Mill. Under Unheated Greenhouse

    Directory of Open Access Journals (Sweden)

    S.M. Alaoui

    2014-01-01

    Full Text Available Grafted Tomatoes were grown on a fine sandy soil using drip irrigation and plastic mulch to evaluate the effects of irrigation scheduling on water requirements and vegetative parameters under typical Massa greenhouses growing conditions. Capacitive sensors were used to automatically schedule irrigations. The result of this study shows that irrigation dose and frequency does not affect stem diameter in grafted tomato plant, no significant effect on leaves number has been observed. But irrigation scheduling have a large effect on root’s development, The root containers results indicated that a water stress equivalent to 50%ETc and 20% frequency can lead to deep root system; that makes possible to sustain a suitable vegetative canopy if doses and frequencies are well managed in a daily scale; It was possible save 50% of irrigation water.

  8. ANALYSIS OF CAROTENOIDS AND LYCOPENE IN TOMATO (LYCOPERSICON ESCULENTUM MILL. AND THEIR RETENTION IN TOMATO JUICE

    Directory of Open Access Journals (Sweden)

    Ján Mareček

    2012-04-01

    Full Text Available 800x600 Normal 0 21 false false false SK X-NONE X-NONE In this work we investigated the effect of variety and processing on the content of carotenoids and lycopene in fruits and Tomato juice from the fruit after heat treatment. The experiment included four varieties are edible tomato for industrial processing (Báb, Žiara PK, Šampion and Roti PK. The concentration of total carotenoids and lycopene were determined spectrophotometrically on UV-VIS spectrophotometer Jenway at a wavelength of 445 and 472 nm. The highest average content of carotenoids in fruits were recorded at a variety Roti PK (7.0 mg/100 g-1 and lowest in variety Báb (4.8 mg/100 g-1. Heat treatment had a statistically significant positive effect on the lycopene content, changes in carotenoid content were not significant. Effect of genotype (variety for the content of the endpoint was significantly important.doi:10.5219/195 Normal 0 21 false false false SK X-NONE X-NONE

  9. Molecular chaperone activity of tomato (Lycopersicon esculentum) endoplasmic reticulum-located small heat shock protein.

    Science.gov (United States)

    Mamedov, Tarlan G; Shono, Mariko

    2008-03-01

    The gene encoding the small heat shock protein (sHSP), LeHSP21.5, has been previously cloned from tomato (GenBank accession no. AB026983). The deduced amino acid sequence of this tomato sHSP was most similar to that of other endoplasmic reticulum (ER)-localized sHSPs (ER-sHSP) and can be predicted to target the ER. We examined whether the gene product of LeHSP21.5 (probable ER-sHSP) can act as molecular chaperone. For functional analysis, LeHSP21.5 protein was expressed in Escherichia coli as His(6)-tagged protein in the C-terminal and purified. We confirmed that ER-sHSP could provide thermal protection of soluble proteins in vitro. We compared the thermal stability of E. coli strain BL21 (DE3) transformed with pET-ER-sHSP with the control E. coli strain BL21(DE3) transformed with only the pET vector under heat shock and IPTG-induced conditions. Most of the protein extracts from E. coli cells expressing ER-sHSP were protected from heat-induced denaturation, whereas extracts from cells not expressing ER-sHSP were very heat-sensitive under these conditions. A similar protective effect was observed when purified ER-sHSP was added to an E. coli cell extract. ER-sHSP prevented the thermal aggregation and inactivation of citrate synthase. These collective findings indicate that ER-sHSP can function as a molecular chaperone in vitro.

  10. Enzymatic properties of chorismate synthase isozymes of tomato (Lycopersicon esculentum Mill.).

    Science.gov (United States)

    Braun, M; Henstrand, J M; Görlach, J; Amrhein, N; Schmid, J

    1996-01-01

    Three plastidic chorismate synthase isozymes (CS1, CS2 and CS2 delta) of tomato were identified by isolation of the corresponding cDNAs. These three cDNAs are derived from only two genes (LeCS1 and LeCS2). This additional complexity results from differential splicing of the primary transcript of one of the genes (LeCS2) giving rise to two different transcripts (CS2 and CS2 delta transcripts). All three isozymes were individually expressed in Escherichia coli both as precursor proteins with N-terminal transit peptides and as mature proteins. Only the mature but not the precursor isozymes CS1 and CS2 were enzymatically active. The enzyme CS2 delta was unstable in E. coli. Both CS1 and CS2 were purified to near homogeneity and their enzymatic properties were analyzed. They differ substantially in their Km values for the substrate 5-enol-pyruvylshikimate 3-phosphate (11 and 80 microM for the mature forms of CS1 and CS2, respectively). The two isozymes appear to be active only as oligomers, and the potential physiological implications of this result are discussed.

  11. Preferential Promotion of Lycopersicon esculentum (Tomato) Growth by Plant Growth Promoting Bacteria Associated with Tomato.

    Science.gov (United States)

    Vaikuntapu, Papa Rao; Dutta, Swarnalee; Samudrala, Ram Babu; Rao, Vukanti R V N; Kalam, Sadaf; Podile, Appa Rao

    2014-12-01

    A total of 74 morphologically distinct bacterial colonies were selected during isolation of bacteria from different parts of tomato plant (rhizoplane, phylloplane and rhizosphere) as well as nearby bulk soil. The isolates were screened for plant growth promoting (PGP) traits such as production of indole acetic acid, siderophore, chitinase and hydrogen cyanide as well as phosphate solubilization. Seven isolates viz., NR4, NR6, RP3, PP1, RS4, RP6 and NR1 that exhibited multiple PGP traits were identified, based on morphological, biochemical and 16S rRNA gene sequence analysis, as species that belonged to four genera Aeromonas, Pseudomonas, Bacillus and Enterobacter. All the seven isolates were positive for 1-aminocyclopropane-1-carboxylate deaminase. Isolate NR6 was antagonistic to Fusarium solani and Fusarium moniliforme, and both PP1 and RP6 isolates were antagonistic to F. moniliforme. Except RP6, all isolates adhered significantly to glass surface suggestive of biofilm formation. Seed bacterization of tomato, groundnut, sorghum and chickpea with the seven bacterial isolates resulted in varied growth response in laboratory assay on half strength Murashige and Skoog medium. Most of the tomato isolates positively influenced tomato growth. The growth response was either neutral or negative with groundnut, sorghum and chickpea. Overall, the results suggested that bacteria with PGP traits do not positively influence the growth of all plants, and certain PGP bacteria may exhibit host-specificity. Among the isolates that positively influenced growth of tomato (NR1, RP3, PP1, RS4 and RP6) only RS4 was isolated from tomato rhizosphere. Therefore, the best PGP bacteria can also be isolated from zones other than rhizosphere or rhizoplane of a plant.

  12. Pre-meal tomato (Lycopersicon esculentum) intake can have anti-obesity effects in young women?

    Science.gov (United States)

    Vinha, Ana F; Barreira, Sérgio V P; Costa, Anabela S G; Alves, Rita C; Oliveira, M Beatriz P P

    2014-12-01

    The effect of pre-meal tomato intake in the anthropometric indices and blood levels of triglycerides, cholesterol, glucose, and uric acid of a young women population (n = 35, 19.6 ± 1.3 years) was evaluated. During 4 weeks, daily, participants ingested a raw ripe tomato (∼90 g) before lunch. Their anthropometric and biochemical parameters were measured repeatedly during the follow-up time. At the end of the 4 weeks, significant reductions were observed on body weight (-1.09 ± 0.12 kg on average), % fat (-1.54 ± 0.52%), fasting blood glucose (-5.29 ± 0.80 mg/dl), triglycerides (-8.31 ± 1.34 mg/dl), cholesterol (-10.17 ± 1.21 mg/dl), and uric acid (-0.16 ± 0.04 mg/dl) of the participants. The tomato pre-meal ingestion seemed to interfere positively in body weight, fat percentage, and blood levels of glucose, triglycerides, cholesterol, and uric acid of the young adult women that participated in this study.

  13. Dissipation and decontamination of bifenthrin residues in tomato (Lycopersicon esculentum Mill).

    Science.gov (United States)

    Chauhan, Reena; Monga, Samriti; Kumari, Beena

    2012-07-01

    A field experiment was conducted at the Research Farm of CCS HAU, Hisar to study the dissipation and decontamination behavior of bifenthrin on tomato crop following the application of 25 g a.i ha(-1) (T(1)) and 50 g a.i ha(-1) (T(2)). Samples were collected periodically on the sampling days after applications. Residues were reached below detectable level of 0.005 mg kg(-1) on 10(th) day after application showing half-life period of 1.83 and 2.05 days at room temperature and 2.02 and 2.32 days under refrigerated condition for single and double dose, respectively. Processing was found effective in reducing the residues of bifenthrin in tomato fruits. Maximum reduction (42.10-45.23 %) was observed by washing + boiling followed by washing (16.66-19.04 %). Reduction was slightly less when samples were stored under refrigerated conditions as compared to room temperature conditions.

  14. [Callose accumulation during treatment of tomato (Lycopersicon esculentum L.) cells with biotic elicitors].

    Science.gov (United States)

    Emel'ianov, V I; Kravchuk, Zh N; Poliakovskiĭ, S A; Dmitriev, A P

    2008-01-01

    Time-course of induced accumulation of callose in tomato cells has been studied. Localization of callose in L. esculenthum cells was investigated by fluorescent microscopy technique, and the optimal time for its determination was found. Callose accumulation in tomato cells treated with different biotic elicitors was determined. Nonlinear dependence between callose accumulation and concentration of chitin oligomers (with 3-5 N-acetylglucosamine fragments) was established. Increasing of callose accumulation in tomato cells was proportional to the increase of concentration ofchitin dimer and chitosan in the culture medium.

  15. Effect of partial root drying on growth and photosynthesis of tomato (Lycopersicon esculentum L.

    Directory of Open Access Journals (Sweden)

    Savić Slađana

    2004-01-01

    Full Text Available Tomato plants were grown in commercial compost with the root system divided equally in two parts (PRD technique. At the end of vegetative and during generative stage of development, half of the root system was exposed to drought, while the remainder of the root system was irrigated. One PRD treatment took c.10 days and during this period the soil water content in the dry root side was reduced to 30%. After this, the treatment was reversed, allowing the previously dry compartment to be well-watered and the well-watered compartment to dry down. In control plants both compartments were watered daily to drip point throughout the experimental period. During experimental period the following measurements were done: plant height, leaf number and area, number of flower trusses, number and diameter of fruit leaf gas exchange (photosynthesis and transpiration, leaf water potential leaf apoplastic pH and water-use efficiency (WUE. Obtained results of plants height and leaf number and area showed that, as a consequence of PRD treatment, the growth of whole plants was reduced, but not fruit, although WUE was increased. These results pointed out that with PRD technique it is possible to reduce irrigation water without significant reduction effect on tomato yield.

  16. Detection and molecular characterization of tomato yellow leaf curl virus naturally infecting Lycopersicon esculentum in Egypt.

    Science.gov (United States)

    Rabie, M; Ratti, C; Abdel Aleem, E; Fattouh, F

    2017-01-01

    Tomato yellow leaf curl virus (TYLCV) infections of tomato crops in Egypt were widely spread in 2014. Infected symptomatic tomato plants from different governorates were sampled. TYLCV strains Israel and Mild (TYLCV-IL, TYLCV-Mild) were identified by multiplex and real-time PCR. In addition, nucleotide sequence analysis of the V1 and V2 protein genes, revealed ten TYLCV Egyptian isolates (TYLCV from TY1 to 10). Phylogenetic analysis showed their high degree of relatedness with TYLCV-IL Jordan isolate (98%). Here we have showed the complete nucleotide sequence of the TYLCV Egyptian isolate TY10, sampled from El Beheira. A high degree of similarity to other previously reported Egyptian isolates and isolates from Jordan and Japan reflect the importance of phylogenetic analysis in monitoring virus genetic diversity and possibilities for divergence of more virulent strains or genotypes.

  17. Heat tolerance in Field Grown Tomatoes (Lycopersicon esculentum Mill.) under Semi Arid Conditions of West Africa

    DEFF Research Database (Denmark)

    Kugblenu, Y O; Oppong Danso, E; Ofori, K;

    2013-01-01

    One major reason for extremely low production of tomato in Ghana is that the length of the growing season last only for a few months due to the high temperature influx during the remaining months. The temperatures recorded during these months are above the optimum for tomato flowering and fruiting...

  18. Estudo teórico e experimental de secagem de tomate (Lycopersicon esculentum)

    OpenAIRE

    Luiz, Marcia Ramos

    2012-01-01

    Secagem de alimentos é um processo utilizado para assegurar a qualidade e a estabilidade dos alimentos, considerando que a diminuição da quantidade de água do material reduz a atividade biológica e as mudanças químicas e físicas que ocorrem durante o armazenamento. O objetivo deste trabalho foi estudar a transferência de calor e massa na secagem de tomate cortado em fatias e avaliar as características da cinética de secagem em dois equipamentos resistivos e uma bomba de calor. ...

  19. Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill. mutant

    Directory of Open Access Journals (Sweden)

    Elizanilda R. do Rêgo

    1999-03-01

    Full Text Available A naturally occurring yellow tomato fruit mutant cv. Santa Clara was reciprocally crossed with the red wild type, after which F1 plants were self pollinated or backcrossed with both parents. Plants from F1 generations produced all fruits with a homogeneous deep red color when ripe. F2 plants showed a 3:1 red:yellow segregation of fruit color, and 100% red when backcrossed with red wild type or 1:1 red:yellow segregation in backcrosses with the yellow mutant; hence, yellow fruit color was determined by a recessive allele. Based on reciprocal crosses, fruit color is unlikely to be determined by maternal genes. Accumulation of lycopene dropped by 99.3% and b-carotene by 77% in ripe yellow fruits, compared to the red wild type. Leaf and flower chlorophyll and total carotenoid concentrations were not affected by the yellow mutation. However, the mutant fruit had a higher rate of chlorophyll degradation during fruit ripening, whilst fruit from the F1 generation showed lower rates of degradation, similar to that observed in red wild type fruits.Neste trabalho avaliou-se a herança da cor do fruto de um mutante natural da cv. Santa Clara, por meio da análise das gerações F1 e segregantes, obtidas mediante cruzamento entre plantas da cv. Santa Clara normal e o mutante amarelo. A caracterização das plantas normais, mutantes e F1 foi feita com base na análise quantitativa dos pigmentos carotenóides e clorofila em flores, folhas e frutos verdes e maduros. Plantas F1 e provenientes do retrocruzamento com o progenitor normal apresentaram 100% de frutos vermelhos. A semelhança entre os F1 recíprocos mostra que há ausência de herança materna para as características avaliadas. Em gerações segregantes, as freqüências observadas foram compatíveis com herança monogênica pelo teste qui-quadrado, com dominância completa para o gene que confere cor vermelha. Os frutos amarelos apresentaram teores reduzidos de b-caroteno e licopeno, enquanto o híbrido apresentou teores intermediários desses carotenóides quando comparados com o genótipo normal. Os níveis de clorofila em frutos verde-maduros e maduros mutantes foram menores que nos frutos normais, evidenciando o papel dos carotenóides sobre a fotoproteção da clorofila. A concentração de clorofila e carotenóides, em folhas e flores, não foi afetada pela mutação.

  20. EFEITO DE TRICOMAS, ALELOQUÍMICOS E NUTRIENTES NA RESISTÊNCIA DE LYCOPERSICON HIRSUTUM À TRAÇA-DO-TOMATEIRO EFFECT OF TRICHOMES, ALELLOCHEMICALS AND MINERALS ON THE RESISTANCE OF LYCOPERSICON HIRSUTUM TO TOMATO LEAF MINER

    Directory of Open Access Journals (Sweden)

    GERMANO LEÃO DEMOLIN LEITE

    1999-11-01

    Full Text Available Estudos foram conduzidos com o objetivo de verificar o efeito de tricomas, aleloquímicos e nutrientes nas folhas de partes do dossel das plantas na resistência de Lycopersicon hirsutum à traça- do-tomateiro, Tuta absoluta (Lepidoptera: Gelechiidae. Foram quantificados os teores de 2-tridecanona (2-TD, 2-undecanona (2-UD, N, P, K, Ca e Mg, densidade e tipos de tricomas e tamanho das folhas nos terços apical, mediano e basal do dossel de plantas de L. hirsutum e de L. esculentum e estudaram- se os efeitos destes fatores sobre características biológicas de T. absoluta. Observou-se elevação no teor de 2-TD da base para o ápice do dossel. Não se detectou diferença significativa quanto ao número de ovos de T. absoluta ao longo do dossel de L. hirsutum, constatando-se em L. esculentum maior oviposição nos terços apical e mediano do que no basal. As folhas do terço apical de L. hirsutum apresentaram maior efeito deletério sobre as larvas de traça.The objective of this work was to study the effect of trichomes, alellochemicals and minerals in the leaves of different canopy heights on the resistance of Lycopersicon hirsutum to tomato leaf miner, Tuta absoluta (Lepidoptera: Gelechiidae. Effects of 2-tridecanone (2-TD, 2-undecanone (2-UD, N, P, K, Ca and Mg levels, density and types of trichomes and leaf area on apical, medium and basal parts of plant dossel of L. hirsutum and L. esculentum on the oviposition and mines number of T. absoluta was studied. Production of 2-TD increased from the bottom to the top of the canopy. The apical part of plants of L. hirsutum showed more antibiotic effect on the caterpillar. T. absoluta oviposited more on leaves of the apical and medium portion of the plants than in the basal parts of L. esculentum.

  1. Anti-ulcer potential of Oxystelma esculentum

    Directory of Open Access Journals (Sweden)

    Devang J Pandya

    2011-01-01

    Full Text Available Oxystelma esculentum is a perennial twiner growing near water-logged areas in the Indian subcontinent. It is used traditionally in stomach ulcers. The present work deals with the investigation of anti-ulcer potential of O. esculentum. The plant was successively extracted with solvents of varying polarities, which served as the test extracts. Anti-ulcer effect was checked in Wistar rats using aspirin- and ethanol-induced acute ulcer models. The petroleum ether extract was found to possess the most effective anti-ulcer activity. This proves the traditional claim of the plant as an anti-ulcer drug. Phytochemical screening of this extract revealed the presence of important classes of compounds like cardenolides, flavonoids, phenolics, sterols and triterpenoids. This bioactivity-guided phytochemical screening can guide further therapeutic investigations and isolation of pharmacologically active compounds from Oxystelma esculentum.

  2. ISOLATION OF EPICATECHIN FROM OXYSTELMA ESCULENTUM

    Directory of Open Access Journals (Sweden)

    D.J. Pandya* and I.S. Anand

    2013-01-01

    Full Text Available Background: Oxystelma esculentum is used traditionally as diuretic, laxative, hepatoprotective and anti-ulcer.Objectives: The present work deals with the isolation and structure elucidation of Epicatechin from O. esculentum. Methods: Methanolic extract of the entire plant of O. esculentum was subjected to pilot TLC experiments and column chromatography. Results: This yielded a pure, white compound which resolved at Rf 0.3 on TLC plate using the mobile phase toluene: ethyl acetate (5:4 when developed in iodine chamber. This compound was subjected to UV, IR and 1H-NMR spectral analysis and its structure elucidation revealed it to be Epicatechin.Conclusion: This compound has been reported for the first time in this plant and can serve as a useful tool in its standardization.

  3. Two Loci from Lycopersicon hirsutum LA407 Confer Resistance to Strains of Clavibacter michiganensis subsp. michiganensis.

    Science.gov (United States)

    Kabelka, E; Franchino, B; Francis, D M

    2002-05-01

    ABSTRACT We used molecular markers to identify quantitative trait loci (QTL) that contribute to resistance to bacterial canker of tomato caused by Clavibacter michiganensis subsp. michiganensis. Resistance was first identified as a marker-trait association in an inbred backcross (IBC) population derived from crossing Lycopersicon hirsutum accession (LA407) with L. esculentum. Single-marker QTL analysis suggested that at least two loci originating from L. hirsutum LA407, Rcm 2.0 on chromosome 2 and Rcm 5.1 on chromosome 5, contribute to resistance in replicated trials. Two segregating F(2) populations were developed by crossing resistant inbred backcross lines (IBLs) to elite L. esculentum lines and used to confirm QTL associations detected in the IBC population. In these populations, realized heritability estimates were higher for selection based on maximal disease than for selection based on disease progression. Realized heritability in the population carrying Rcm 2.0 was 0.63 and 0.14, respectively, for each selection criteria. Realized heritability estimates were 0.85 for selection based on maximal disease and 0.37 for selection based on disease progression in a population carrying Rcm 5.1. The disease response of F(3) families selected for resistance suggested that both Rcm 2.0 and Rcm 5.1 confer resistance to bacterial strains in the repetitive sequence-based polymerase chain reaction DNA fingerprint classes A and C. Markers linked to Rcm 2.0 explained up to 56% of the total phenotypic variation for resistance in one population, and markers linked to Rcm 5.1 explained up to 73% of the total phenotypic variation for resistance in a separate population.

  4. BARLEY CULTIVARS

    African Journals Online (AJOL)

    1993-06-13

    Jun 13, 1993 ... models were each based on several parametric statistics: 1. Thevarianceofacultivaracrossenvironments. This approach uses the variance of a cultivar ... The predicted yield values areadjusted ... Subsistence farmers risks.

  5. Mathematical modelling of the osmotic dehydration of cherry tomato (Lycopersicon esculentum var. cerasiforme Modelagem matemática da desidratação osmótica de tomate cereja (Lycopersicon esculentum var. cerasiforme

    Directory of Open Access Journals (Sweden)

    Patricia Moreira AZOUBEL

    2000-08-01

    Full Text Available Osmotic dehydration of cherry tomato as influenced by osmotic agent (sodium chloride and a mixed sodium chloride and sucrose solutions and solution concentration (10 and 25% w/w at room temperature (25°C was studied. Kinetics of water loss and solids uptake were determined by a two parameter model, based on Fick's second law and applied to spherical geometry. The water apparent diffusivity coefficients obtained ranged from 2.17x10-10 to 11.69x10-10 m²/s.O presente trabalho teve como objetivo o estudo da influência do agente osmótico (cloreto de sódio e a mistura cloreto de sódio-sacarose e da concentração da solução (10 e 25% p/p na desidratação osmótica de tomate cereja na temperatura ambiente (25°C. A cinética de perda de água foi determinada através de uma equação de dois parâmetros, baseada na segunda lei de Fick e aplicada para uma geometria esférica. Os coeficientes de difusividade aparente obtidos ficaram na faixa de 2,17x10-10 a 11,69x10-10 m²/s.

  6. CRECIMIENTO DE PLANTAS DE TOMATE (Lycopersicon esculentum Mill) EN UN SUELO ROJO COMPACTADO TRATADO CON LODO DE DEPURADORA

    OpenAIRE

    E. Utria; Inés Reynaldo; Cabrera, A.; Ada Morúa

    2004-01-01

    Con el objetivo de evaluar y comparar la respuesta de algunos componentes del crecimiento en plantas de tomate, en un suelo Ferralítico Rojo compactado tratado con varias dosis de lodo de depuradora, se realizó el presente trabajo en el Instituto Nacional de Ciencias Agrícolas. Se hizo un experimento que se repitió por dos años, en los cuales los resultados mostraron un incremento de la altura de la planta, diámetro del tallo, superficie foliar, masa seca de la parte aérea y radical de las pl...

  7. Evaluation of two composts for the improvement of crop yield using tomato (Lycopersicon esculentum as test crop

    Directory of Open Access Journals (Sweden)

    Fawole Oluyemisi B.

    2016-01-01

    Full Text Available In search of a more environmentally friendly alternative to the use of chemical fertilizers, a study was conducted to evaluate the use of compost for improved crop productivity. We compared the succession of microorganisms in the compost heaps using hot bed method of composting. They contained grass clippings, sawdust, NPK fertilizer, ashes, corn cobs, bean chaff, vegetable stalks, newspaper shreds and soil arranged in layers in a round structure. Poultry dropping was the organic nitrogen source of one heap while pig waste was used for the other heap. Samples were taken weekly and analyzed using soil dilution method for isolation of moulds on potato dextrose agar medium. The qualities of composts after eight weeks were evaluated by performance and yield of tomato crops. Eleven fungal isolates were obtained in compost containing poultry dropping and nine fungal isolates were obtained from compost containing pig manure. The predominant mycoflora of poultry dropping compost at 3 weeks of composting was Fusarium pallidoroseum (23.08% while Aspergillus fumigatus (38.96% dominated compost containing pig waste. Fungi isolated from the composts included cellulolytic fungi like Chaetomium sp. and Phoma sp. Soil amended with both composts improved the growth and yield of tomato crop significantly. It was concluded that compost containing poultry droppings was richer and therefore encouraged higher microbial activity than compost containing pig waste. Knowledge of the microbial succession during composting and conditions required could further be employed to enhance composting.

  8. Cultivation Methods for Tomato (Lycopersicon esculentum Mill.) with High Soluble Solids Content Using the Vertically Split Root System

    National Research Council Canada - National Science Library

    HAYASHI, Hiroyuki; TOYOFUKU, Kyoko; TAGUCHI, Takiko; OGAWA, Atsushi

    2014-01-01

    For the cultivation of tomatoes with high soluble solids content, the root system was split vertically and irrigation to the upper part of the root system was stopped to create split vertically (SR treatment...

  9. Composting Phragmites australis Cav. plant material and compost effects on soil and tomato (Lycopersicon esculentum Mill.) growth.

    Science.gov (United States)

    Toumpeli, Anna; Pavlatou-Ve, Athina K; Kostopoulou, Sofia K; Mamolos, Andreas P; Siomos, Anastasios S; Kalburtji, Kiriaki L

    2013-10-15

    Composting organic residues is a friendly to the environment alternative to producing fertilizer. This research was carried out to study the process of composting Phragmites australis Cav. plant material alone or with animal manure on a pilot-scale, to evaluate firstly the quality of the composts produced and secondly, using a pot experiment, the effects of their application on soil physicochemical characteristics and tomato plants development. For the compost production a randomized complete block design was used with five treatments (five compost types) and four replications. For the pot experiment, a completely randomized design was used with 17 treatments (plain soil, soil with synthetic fertilizer and the application of five compost types, at three rates each) and five replications. Compost N increased with composting time, while C/N ratio decreased significantly and by the end it ranged from 43.3 for CM to 22.6 for CY. Compost pH became almost neutral, ranging from 6.73 for CY to 7.21 for CM3Y3AM4 by the end. Compost combinations CY7AM3 and CM7AM3 had a more positive influence on the soil physicochemical characteristics than the others. Soil N, P, Ca and Mg concentrations and the reduction of clay dispersion were the highest when CM7AM3 compost was added. The macro-aggregate stability was the highest for CY7AM3, which also sustained plant growth. The latter compost combination improved most of the soil physicochemical characteristics and plant growth especially, when the application rate was 4% (w/w), which equals to 156 Mg ha(-1). Copyright © 2013 Elsevier Ltd. All rights reserved.

  10. The distribution of ATP within tomato (Lycopersicon esculentum Mill.) embryos correlates with germination whee as total ATP concentration does not

    NARCIS (Netherlands)

    Spoelstra, P.; Joosen, R.V.L.; Hilhorst, H.W.M.

    2002-01-01

    The distribution of ATP in tomato seeds was visualized by monitoring the luminescence of frozen sections on top of a gel containing all the components of the luciferase reaction, but excluding ATP. ATP was imaged in germinating tomato seeds at intervals of 3, 6, 17, 24 and 48 h and in seeds with pri

  11. Tomato (Lycopersicon esculentum, cv CXD271BIO) yield and quality during converion from conventional to organic production

    OpenAIRE

    Nardo, N.; Foddai, MS; E.Azzini; Baiamonte, I; Di Ferdinando, S; Paoletti, S.; Vizioli, V; Paoletti, F.

    2011-01-01

    The conversion of a conventional farm to the methods of the organic farming is a very critical stage of technical and economical investment. From an agronomical point of view, usually the conversion needs a very different length of time with respect to that set by the regulation, because the organic farming is based on the soil fertility and, in general, on the preservation at the equilibrium of the farm’s ecosystem. These elements affect time and method of the conversions that, although base...

  12. The distribution of ATP within tomato (Lycopersicon esculentum Mill.) embryos correlates with germination whee as total ATP concentration does not

    NARCIS (Netherlands)

    Spoelstra, P.; Joosen, R.V.L.; Hilhorst, H.W.M.

    2002-01-01

    The distribution of ATP in tomato seeds was visualized by monitoring the luminescence of frozen sections on top of a gel containing all the components of the luciferase reaction, but excluding ATP. ATP was imaged in germinating tomato seeds at intervals of 3, 6, 17, 24 and 48 h and in seeds with

  13. AVALIAÇÃO DE PROGÊNIES DE TOMATE (LYCOPERSICON ESCULENTUM) PARA CULTIVO SOB TEMPERATURAS ELEVADAS

    OpenAIRE

    Noda, Hiroshi; MACHADO,Francisco Manoares

    1992-01-01

    Foram avaliadas três progênies F7 de tomate άο cruzamento HT-16, do programa de melhoramento genético do Departamento de Ciências Agronômicas do INPA, para o caráter pegamento de frutos. Os ensaios foram realizados em casa de vegetação com cobertura de plástico transparente e por um período de 37 dias, que cobriu toda a fase de florescimento e frutificação do primeiro ao oitavo racimo; tomou-se dados sobre a temperatura do ar no interior da casa de vegetação, por meio de um termohig...

  14. Biochar filters reduced the toxic effects of nickel on tomato (Lycopersicon esculentum L.) grown in nutrient film technique hydroponic system.

    Science.gov (United States)

    Mosa, Ahmed; El-Banna, Mostafa F; Gao, Bin

    2016-04-01

    This work used the nutrient film technique to evaluate the role of biochar filtration in reducing the toxic effects of nickel (Ni(2+)) on tomato growth. Three hydroponic treatments: T1 (control), T2 (with Ni(2+)), and T3 (with Ni(2+) and biochar) were used in the experiments. Scanning electron microscopy equipped with energy dispersive X-ray spectroscopy and Fourier transform spectroscopy was used to characterize the pre- and post-treatment biochar samples. The results illustrated that precipitation, ion exchange, and complexation with surface functional groups were the potential mechanisms of Ni(2+) removal by biochar. In comparison to the control, the T2 treatment showed severe Ni-stress with alterations in cell wall structure, distortions in cell nucleus, disturbances in mitochondrial system, malformations in stomatal structure, and abnormalities in chloroplast structure. The biochar filters in T3 treatment reduced dysfunctions of cell organelles in root and shoot cells. Total chlorophyll concentration decreased by 41.6% in T2 treatment. This reduction, however, was only 20.8% due to the protective effect of the biochar filters. The presence of Ni(2+) in the systems reduced the tomato fruit yield 58.5% and 31.9% in T2 and T3, respectively. Nickel concentrations reached the toxic limit in roots, shoots, and fruits in T2, which were not observed in T3. Biochar filters in T3 also minimized the dramatic reductions in nutrients concentration in roots, shoots, and fruits, which occurred in T2 treatment due to the severe Ni-stress. Findings from this work suggested that biochar filters can be used on farms as a safeguard for wastewater irrigation. Copyright © 2016 Elsevier Ltd. All rights reserved.

  15. CRECIMIENTO DE PLANTAS DE TOMATE (Lycopersicon esculentum Mill) EN UN SUELO ROJO COMPACTADO TRATADO CON LODO DE DEPURADORA

    OpenAIRE

    E. Utria; Inés Reynaldo; Cabrera, A; Ada Morúa

    2004-01-01

    Con el objetivo de evaluar y comparar la respuesta de algunos componentes del crecimiento en plantas de tomate, en un suelo Ferralítico Rojo compactado tratado con varias dosis de lodo de depuradora, se realizó el presente trabajo en el Instituto Nacional de Ciencias Agrícolas. Se hizo un experimento que se repitió por dos años, en los cuales los resultados mostraron un incremento de la altura de la planta, diámetro del tallo, superficie foliar, masa seca de la parte aérea y radical de las pl...

  16. Rapid Inactivation of Chloroplastic Ascorbate Peroxidase is Responsible for Oxidative Modification to Rubisco in Tomato (Lycopersicon esculentum) under Cadmium Stress

    Institute of Scientific and Technical Information of China (English)

    Kai-Lang Liu; Lin Shen; Jia-Qi Wang; Ji-Ping Sheng

    2008-01-01

    To investigate the sensitive site of antioxidant systems in chloroplast under cadmium stress and its consequence on reactive oxygen species production and action, the sub-organellar localization of chloroplast superoxide dismutases (SOD,EC 1.15.1.1) and ascorbic peroxidase (APX, EC 1.11.1.11) isoenzymes and changes of enzymes activities under cadmium stress were investigated in tomato seedlings. Two APX isoforms, one thylakoid-bound and one stromal, were detected. Cd at 50 μM induced a moderate increase of SOD activities but a rapid inactivation of both APX isoenzymes. APX inactivation was mainly related to the decrease of ascorbate concentration, as supported by in vitro treatment of exogenous ascorbate and APX kinetic properties under Cd stress. H2O2 accumulation in chloroplast, as a consequence of APX inactivation,was associated with a 60% loss of Rubisco (EC 4.1.1.39) activity, which could be partially accounted for by a 10% loss of Rubisco content. Protein oxidation assay found that the Rubisco large subunit was the most prominent carbonylated protein; the level of carbonylated Rubisco large subunit increased fivefold after Cd exposure. Thiol groups in the Rubisco large subunit were oxidized, as indicated by non-reducing electrophoresis. Treating crude extract with H2O2 resulted in a similar pattern of protein oxidation and thiols oxidation with that observed in Cd-treated plants. Our study indicates that APXs in the chloroplast is a highly sensitive site of antioxidant systems under Cd stress, and the inactivation of APX could be mainly responsible for oxidative modification to Rubisco and subsequent decrease in its activity.

  17. APLICACIÓN FOLIAR DE ÁCIDO GLUTÁMICO EN PLANTAS DE JITOMATE (Lycopersicon esculentum Mill.)

    OpenAIRE

    José Reynaldo Serna-Rodríguez; Rogelio Castro-Brindis; María Teresa Colinas-León; Jaime Sahagún-Castellanos; Juan Enrique Rodríguez-Pérez

    2011-01-01

    El ácido glutámico es un aminoácido que participa en diversos procesos metabólicos en la planta. El objetivo fue determinar si la aplicación foliar de ácido glutámico produce mejor desarrollo de la planta de jitomate y un incremento en la producción de frutos. El experimento fue desarrollado bajo condiciones de invernadero e hidroponia. Las variables en las cuales hubo respuesta positiva a la aplicación de ácido glutámico fueron: contenido de clorofila b, peso de frutos frescos, peso de fruto...

  18. Effect of some commonly used pesticides on seed germination, biomass production and photosynthetic pigments in tomato (Lycopersicon esculentum).

    Science.gov (United States)

    Shakir, Shakirullah Khan; Kanwal, Memoona; Murad, Waheed; Zia ur Rehman; Shafiq ur Rehman; Daud, M K; Azizullah, Azizullah

    2016-03-01

    Pesticides are highly toxic substances. Their toxicity may not be absolutely specific to the target organisms but can adversely affect different processes in the non-target host plants. In the present study, the effect of over application of four commonly used pesticides (emamectin benzoate, alpha-cypermethrin, lambda-cyhalothrin and imidacloprid) was evaluated on the germination, seedling vigor and photosynthetic pigments in tomato. The obtained results revealed that seed germination was decreased by the pesticides and this effect was more prominent at early stages of exposure. All the tested pesticides reduced the growth of tomato when applied in higher concentration than the recommended dose, but at lower doses the pesticides had some stimulatory effects on growth as compared to the control. A similar effect of pesticides was observed on the photosynthetic pigments, i.e. a decrease in pigments concentrations was caused at higher doses but an increase was observed at lower doses of pesticides. The calculation of EC50 values for different parameters revealed the lowest EC50 values for emamectin (ranged as 51-181 mg/L) followed by alpha-cypermethrin (191.74-374.39), lambda-cyhalothrin (102.43-354.28) and imidacloprid (430.29-1979.66 mg/L). A comparison of the obtained EC50 values for different parameters of tomato with the recommended doses revealed that over application of these pesticides can be harmful to tomato crop. In a few cases these pesticides were found toxic even at the recommended doses. However, a field based study in this regard should be conducted to further verify these results.

  19. Solanum tuberosum and Lycopersicon esculentum Leaf Extracts and Single Metabolites Affect Development and Reproduction of Drosophila melanogaster.

    Directory of Open Access Journals (Sweden)

    Emanuela Ventrella

    Full Text Available Glycoalkaloids are secondary metabolites commonly found in Solanaceae plants. They have anti-bacterial, anti-fungal and insecticidal activities. In the present study we examine the effects of potato and tomato leaf extracts and their main components, the glycoalkaloids α-solanine, α-chaconine and α-tomatine, on development and reproduction of Drosophila melanogaster wild-type flies at different stages. Parental generation was exposed to five different concentrations of tested substances. The effects were examined also on the next, non-exposed generation. In the first (exposed generation, addition of each extract reduced the number of organisms reaching the pupal and imaginal stages. Parent insects exposed to extracts and metabolites individually applied showed faster development. However, the effect was weaker in case of single metabolites than in case of exposure to extracts. An increase of developmental rate was also observed in the next, non-exposed generation. The imagoes of both generations exposed to extracts and pure metabolites showed some anomalies in body size and malformations, such as deformed wings and abdomens, smaller black abdominal zone. Our results further support the current idea that Solanaceae can be an impressive source of molecules, which could efficaciously be used in crop protection, as natural extract or in formulation of single pure metabolites in sustainable agriculture.

  20. Solanum tuberosum and Lycopersicon esculentum Leaf Extracts and Single Metabolites Affect Development and Reproduction of Drosophila melanogaster

    Science.gov (United States)

    Ventrella, Emanuela; Adamski, Zbigniew; Chudzińska, Ewa; Miądowicz-Kobielska, Mariola; Marciniak, Paweł; Büyükgüzel, Ender; Büyükgüzel, Kemal; Erdem, Meltem; Falabella, Patrizia; Scrano, Laura; Bufo, Sabino Aurelio

    2016-01-01

    Glycoalkaloids are secondary metabolites commonly found in Solanaceae plants. They have anti-bacterial, anti-fungal and insecticidal activities. In the present study we examine the effects of potato and tomato leaf extracts and their main components, the glycoalkaloids α-solanine, α-chaconine and α-tomatine, on development and reproduction of Drosophila melanogaster wild-type flies at different stages. Parental generation was exposed to five different concentrations of tested substances. The effects were examined also on the next, non-exposed generation. In the first (exposed) generation, addition of each extract reduced the number of organisms reaching the pupal and imaginal stages. Parent insects exposed to extracts and metabolites individually applied showed faster development. However, the effect was weaker in case of single metabolites than in case of exposure to extracts. An increase of developmental rate was also observed in the next, non-exposed generation. The imagoes of both generations exposed to extracts and pure metabolites showed some anomalies in body size and malformations, such as deformed wings and abdomens, smaller black abdominal zone. Our results further support the current idea that Solanaceae can be an impressive source of molecules, which could efficaciously be used in crop protection, as natural extract or in formulation of single pure metabolites in sustainable agriculture. PMID:27213896

  1. Clay pot irrigation for tomato (Lycopersicon esculentum Mill production in the north east semiarid region of Ethiopia

    Directory of Open Access Journals (Sweden)

    Kebede Woldetsadik

    2011-06-01

    Full Text Available Water shortage is one of the major constraints for production of horticultural crops in arid and semiarid regions. A field experiment was conducted to determine irrigation water and fertilizer use efficiency, growth and yield of tomato under clay pot irrigation at the experimental site of Sekota Dryland Agricultural Research Center, Lalibela, Ethiopia in 2009/10. The experiment comprised of five treatments including furrow irrigated control and clay pot irrigation with different plant population and fertilization methods, which were arranged in Randomized Complete Block Design with three replications. The highest total and marketable fruit yields were obtained from clay pot irrigation combined with application of nitrogen fertilizer with irrigation water irrespective of difference in plant population. The clay pot irrigation had seasonal water use of up to 143.71 mm, which resulted in significantly higher water use efficiency (33.62 kg m-3 as compared to the furrow irrigation, which had a seasonal water use of 485.50 mm, and a water use efficiency of 6.67 kg m-3. Application of nitrogen fertilizer with irrigation water in clay pots improved fertilizer use efficiency of tomato by up to 52% than band application with furrow or clay pot irrigation. Thus, clay pot irrigation with 33,333 plants ha-1 and nitrogen fertilizer application with irrigation water in clay pots was the best method for increasing the yield of tomato while economizing the use of water and nitrogen fertilizer in a semiarid environment.

  2. Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling*#

    Science.gov (United States)

    Diao, Qian-Nan; Song, Yong-Jun; Shi, Dong-Mei; Qi, Hong-Yan

    2016-01-01

    Polyamines (PAs) and nitric oxide (NO) are vital signals in modulating plant response to abiotic stress. However, to our knowledge, studies on the relationship between NO and PAs in response to cold stress in tomato are limited. Accordingly, in this study, we investigated the effects of putrescine (Put) and spermidine (Spd) on NO generation and the function of Spd-induced NO in the tolerance of tomato seedling under chilling stress. Spd increased NO release via the nitric oxide synthase (NOS)-like and nitrate reductase (NR) enzymatic pathways in the seedlings, whereas Put had no such effect. Moreover, H2O2 might act as an upstream signal to stimulate NO production. Both exogenous NO donor (sodium nitroprusside (SNP)) and Spd enhanced chilling tolerance in tomato, thereby protecting the photosynthetic system from damage. Compared to chilling treatment alone, Spd enhanced the gene expressions of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and their enzyme activities in tomato leaves. However, a scavenger or inhibitor of NO abolished Spd-induced chilling tolerance and blocked the increased expression and activity due to Spd of these antioxidant enzymes in tomato leaves under chilling stress. The results showed that NO induced by Spd plays a crucial role in tomato’s response to chilling stress. PMID:27921397

  3. GERMINATION PERFORMANCE OF SEQUENTIALLY HARVESTED TOMATO (LYCOPERSICON ESCULENTUM MILL.) SEED LOTS DURING SEED DEVELOPMENT UNDER SALT AND OSMOTIC STRESS

    OpenAIRE

    OZCOBAN, Mustafa; Demir, Ibrahim

    2006-01-01

    This work concluded that tomato seeds harvested 70 days after anthesis showed the maximum germination not only under water but also salt stress. Seeds harvested earlier or later were more sensitive to stress at germinations. High Ki value of the lots indicated high resistance under stress conditions.

  4. GERMINATION PERFORMANCE OF SEQUENTIALLY HARVESTED TOMATO (LYCOPERSICON ESCULENTUM MILL. SEED LOTS DURING SEED DEVELOPMENT UNDER SALT AND OSMOTIC STRESS

    Directory of Open Access Journals (Sweden)

    Mustafa OZCOBAN

    2006-10-01

    Full Text Available This work concluded that tomato seeds harvested 70 days after anthesis showed the maximum germination not only under water but also salt stress. Seeds harvested earlier or later were more sensitive to stress at germinations. High Ki value of the lots indicated high resistance under stress conditions.

  5. Un Micoinsecticida como Agente de Control de la Mosca Blanca (Bemisia argentifolii Bellows y Perrings) en Jitomate (Lycopersicon esculentum Mill.)

    OpenAIRE

    Jesús Loera Gallardo; Ernesto Salgado Sosa

    2004-01-01

    Se evaluó la eficacia del hongo entomopatógeno Beauveria bassiana, elaborado como suspensión emulsificable a concentración de 2.1 x 1010 esporas mL-1 y aplicado en dosis de 1.0, 1.5 y 2.0 L ha-1, contra la mosca blanca Bemisia argentifolii en la variedad Flora Dade de jitomate. Se incluyó como testigo al insecticida endosulfán en concentrado emulsificable (350 g de i.a. L-1) y aplicado en dosis de 2.0 L ha-1. Se evaluó el número de ninfas vivas de mosca blanca encontradas en un área de 2 cm d...

  6. Short term changes in methanol emission and pectin methylesterase activity are not directly affected by light in Lycopersicon esculentum

    Directory of Open Access Journals (Sweden)

    M. T. Lerdau

    2011-04-01

    Full Text Available Plants are an important source of atmospheric methanol (MeOH, the second most abundant organic gas after methane. Factors regulating phytogenic MeOH production are not well constrained in current MeOH emission models. Previous studies have indicated that light may have a direct influence on MeOH production. As light is known to regulate cell wall expansion, it was predicted that light would stimulate MeOH production through the pectin methylesterase (PME pathway. MeOH emissions normalized for stomatal conductance (gs did not, however, increase with light over short time scales (20–30 min. After experimentally controlling for gs and temperature, no light activation of PME activity or MeOH emission was observed. The results clearly demonstrate that light does not directly influence short-term changes in MeOH production and emission. Our data suggest that substrate limitation may be important in regulating MeOH production over short time scales. Future investigation of the long-term impacts of light on MeOH production may increase understanding of MeOH emission dynamics at the seasonal time scale.

  7. Short term changes in methanol emission and pectin methylesterase activity are not directly affected by light in Lycopersicon esculentum

    Directory of Open Access Journals (Sweden)

    M. T. Lerdau

    2011-01-01

    Full Text Available Plants are an important source of atmospheric methanol (MeOH, the second most abundant organic gas after methane. Factors regulating phytogenic MeOH production are not well constrained in current MeOH emission models. Previous studies have indicated that light may have a direct influence on MeOH production. As light is known to regulate cell wall expansion, it was predicted that light would stimulate MeOH production through the pectin methylesterase (PME pathway. MeOH emissions normalized for stomatal conductance (gs did not, however, increase with light over short time scales (20–30 min. After experimentally controlling for gs and temperature, no light activation of PME activity or MeOH emission was observed. The results clearly demonstrate that light does not directly influence short-term changes in MeOH production and emission. Our data suggest that substrate limitation may be important in regulating MeOH production over short time scales. Future investigation of the long-term impacts of light on MeOH production may increase understanding of MeOH emission dynamics at the seasonal time scale.

  8. Impact of Intensity and Exposure Duration of Magnetic Field on Seed Germination of Tomato (Lycopersicon esculentum L.)

    OpenAIRE

    Hassan FEIZI; Hossein SAHABI; Parviz REZVANI MOGHADDAM; Nasser SHAHTAHMASSEBI; Omran GALLEHGIR; Shahram AMIRMORADI

    2012-01-01

    In order to study the effect of seed hydro priming and magnetic field on tomato seed germination an experiment was conducted in laboratory of Ferdowsi University of Mashhad, Iran, in 2010. The experimental treatments were all combinations of two levels of hydro priming (use of dry seed and soaked seed for five h in distilled water) and eight levels of magnetic field treatments (pretreatment of seeds in 15 mT for 5, 15 and 25 minutes, pretreatment of seeds in 25 mT for 5, 15 and 25 minutes, co...

  9. Impact of Intensity and Exposure Duration of Magnetic Field on Seed Germination of Tomato (Lycopersicon esculentum L.

    Directory of Open Access Journals (Sweden)

    Hassan FEIZI

    2012-02-01

    Full Text Available In order to study the effect of seed hydro priming and magnetic field on tomato seed germination an experiment was conducted in laboratory of Ferdowsi University of Mashhad, Iran, in 2010. The experimental treatments were all combinations of two levels of hydro priming (use of dry seed and soaked seed for five h in distilled water and eight levels of magnetic field treatments (pretreatment of seeds in 15 mT for 5, 15 and 25 minutes, pretreatment of seeds in 25 mT for 5, 15 and 25 minutes, continuous magnetic field with 3 mT and control with four replications. Results indicated that hydro priming of seeds reduced mean germination time significantly. Root length increased by 14 percent in seed hydro priming treatment in comparison with dry seed treatment. Shoot length, seedling length and vigor index of hydro primed seeds increased by 7, 12 and 13 percent, respectively compared with dry seeds. Exposure of seeds with 3 mT continuous magnetic field and 25 mT for 5 min increased root length by 29 and 25 percents, respectively in comparison with control. The highest shoot length, seedling length and vigor index were obtained in 3 mT continuous magnetic field and 25 mT for 5 min.

  10. Capítulo II: consideraciones generales sobre el mejoramiento genético de tomate, Lycopersicon esculentum, Mill

    OpenAIRE

    Vallejo Cabrera Franco Alirio

    1994-01-01

    Objetivos del programa de mejoramiento genético del tomate: Los objetivos del programa de mejoramiento dependen de los problemas que se presentan en el cultivo en las diferentes zonas de Colombia, del uso que se le va a dar al fruto (mesa o industria) y especialmente de las condiciones y recursos del agricultor, procesador y consumidor.

  11. Foliar application of calcium chloride and borax influences plant growth, yield, and quality of tomato (Lycopersicon esculentum Mill.) fruit

    OpenAIRE

    RAB, Abdur; Haq, Ihsan-ul

    2012-01-01

    The influence of CaCl2 and borax on growth, yield, and quality of tomato was investigated during the years 2009 and 2010. The experiment was laid out with a randomized complete block design. Calcium chloride (0.3% and 0.6%) and borax (0.2% and 0.4%) solutions were applied as foliar sprays either alone or in combination and data were recorded for plant height, branches per plant, flowers per cluster, fruits per plant, yield, fruit weight, fruit firmness, and total soluble solid content of the ...

  12. Short term changes in methanol emission and pectin methylesterase activity are not directly affected by light in Lycopersicon esculentum

    Science.gov (United States)

    Oikawa, P. Y.; Li, L.; Timko, M. P.; Mak, J. E.; Lerdau, M. T.

    2011-04-01

    Plants are an important source of atmospheric methanol (MeOH), the second most abundant organic gas after methane. Factors regulating phytogenic MeOH production are not well constrained in current MeOH emission models. Previous studies have indicated that light may have a direct influence on MeOH production. As light is known to regulate cell wall expansion, it was predicted that light would stimulate MeOH production through the pectin methylesterase (PME) pathway. MeOH emissions normalized for stomatal conductance (gs) did not, however, increase with light over short time scales (20-30 min). After experimentally controlling for gs and temperature, no light activation of PME activity or MeOH emission was observed. The results clearly demonstrate that light does not directly influence short-term changes in MeOH production and emission. Our data suggest that substrate limitation may be important in regulating MeOH production over short time scales. Future investigation of the long-term impacts of light on MeOH production may increase understanding of MeOH emission dynamics at the seasonal time scale.

  13. Nutritional composition and antioxidant activity of four tomato (Lycopersicon esculentum L.) farmer' varieties in Northeastern Portugal homegardens.

    Science.gov (United States)

    Pinela, José; Barros, Lillian; Carvalho, Ana Maria; Ferreira, Isabel C F R

    2012-03-01

    The nutritional and antioxidant composition of four tomato Portuguese farmer' varieties widely cultivated in homegardens was determined. The analysed components included macronutrients, individual profiles of sugars and fatty acids by chromatographic techniques, hydrophilic antioxidants such as vitamin C, phenolics, flavonols and anthocyanins, and lipophilic antioxidants such as tocopherols, β-carotene and lycopene. Furthermore, the antioxidant activity was evaluated through DPPH scavenging activity, reducing power, β-carotene bleaching inhibition and TBARS formation inhibition. One of the four varieties, which is locally known as round tomato or potato tomato, proved to be the most powerful in antioxidant activity (EC50 values≤1.63 mg/ml), phenolic compounds (phenolics 31.23 mg ClAE/g extract, flavonols 6.36 mg QE/g extract and anthocyanins 3.45 mg ME/g extract) and carotenoids (β-carotene 0.51 mg/100 g and lycopene 9.49 mg/100 g), while the so-called yellow tomato variety revealed interesting nutritional composition, including higher fructose (3.42 g/100 g), glucose (3.18 g/100 g), α-linolenic acid (15.53%) and total tocopherols (1.44 mg/100 g) levels. Overall, these farmer' varieties of garden tomato cultivated in the Northeastern Portuguese region could contribute as sources of important antioxidants related to the prevention of chronic diseases associated to oxidative stress, such as cancer and coronary artery disease.

  14. Accumulation of cell wall-bound phenolic metabolites and their upliftment in hairy root cultures of tomato (Lycopersicon esculentum Mill.).

    Science.gov (United States)

    Mandal, Sudhamoy; Mitra, Adinpunya

    2008-07-01

    Alkaline hydrolysis of cell wall material of tomato hairy roots yielded ferulic acid as the major phenolic compound. Other phenolics were 4-hydroxybenzoic acid, vanillic acid, 4-hydroxybenzaldehyde, vanillin and 4-coumaric acid. The content of phenolics was much higher at the early stage of hairy root growth. The ferulic acid content decreased up to 30 days and then sharply increased to 360 microg/g at 60 days of growth. Elicitation of hairy root cultures with Fusarium mat extract (FME) increased ferulic acid content 4-fold after 24 h. As the pathogen-derived elicitors have specific receptors in plants, FME may thus be used for inducing resistance against Fusarium oxysporum f. sp. lycopersici.

  15. Agronomic properties of wastewater sludge biochar and bioavailability of metals in production of cherry tomato (Lycopersicon esculentum).

    Science.gov (United States)

    Hossain, Mustafa K; Strezov, Vladimir; Chan, K Yin; Nelson, Peter F

    2010-02-01

    This work presents agronomic values of a biochar produced from wastewater sludge through pyrolysis at a temperature of 550 degrees C. In order to investigate and quantify effects of wastewater sludge biochar on soil quality, growth, yield and bioavailability of metals in cherry tomatoes, pot experiments were carried out in a temperature controlled environment and under four different treatments consisting of control soil, soil with biochar; soil with biochar and fertiliser, and soil with fertiliser only. The soil used was chromosol and the applied wastewater sludge biochar was 10tha(-1). The results showed that the application of biochar improves the production of cherry tomatoes by 64% above the control soil conditions. The ability of biochar to increase the yield was attributed to the combined effect of increased nutrient availability (P and N) and improved soil chemical conditions upon amendment. The yield of cherry tomato production was found to be at its maximum when biochar was applied in combination with the fertiliser. Application of biochar was also found to significantly increase the soil electrical conductivity as well as phosphorus and nitrogen contents. Bioavailability of metals present in the biochar was found to be below the Australian maximum permitted concentrations for food.

  16. Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling.

    Science.gov (United States)

    Diao, Qian-Nan; Song, Yong-Jun; Shi, Dong-Mei; Qi, Hong-Yan

    Polyamines (PAs) and nitric oxide (NO) are vital signals in modulating plant response to abiotic stress. However, to our knowledge, studies on the relationship between NO and PAs in response to cold stress in tomato are limited. Accordingly, in this study, we investigated the effects of putrescine (Put) and spermidine (Spd) on NO generation and the function of Spd-induced NO in the tolerance of tomato seedling under chilling stress. Spd increased NO release via the nitric oxide synthase (NOS)-like and nitrate reductase (NR) enzymatic pathways in the seedlings, whereas Put had no such effect. Moreover, H2O2 might act as an upstream signal to stimulate NO production. Both exogenous NO donor (sodium nitroprusside (SNP)) and Spd enhanced chilling tolerance in tomato, thereby protecting the photosynthetic system from damage. Compared to chilling treatment alone, Spd enhanced the gene expressions of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and their enzyme activities in tomato leaves. However, a scavenger or inhibitor of NO abolished Spd-induced chilling tolerance and blocked the increased expression and activity due to Spd of these antioxidant enzymes in tomato leaves under chilling stress. The results showed that NO induced by Spd plays a crucial role in tomato's response to chilling stress.

  17. Residues and dissipation kinetics of carbendazim and diethofencarb in tomato (Lycopersicon esculentum Mill.) and intake risk assessment.

    Science.gov (United States)

    Li, Huidong; du, Hongxia; Fang, Liping; Dong, Zhan; Guan, Shuai; Fan, Wenjing; Chen, Zilei

    2016-06-01

    Dissipation behaviors and residues of carbendazim and diethofencarb in combination in tomato were investigated. The half-lives were 2.1-3.4 days for carbendazim, and 1.8-3.2 days for diethofencarb at a dose of 1.5 times of the recommended dosage. The residues of carbendazim and diethofencarb were below the maximum residue limits (MRLs) in China one day after application of the combination. The ultimate residues were significantly lower than the maximum permissible intake (MPI) in China at the recommended high dose for both child and adult. The values of the maximum dietary exposure for carbendazim and diethofencarb were 0.26 and 0.27 mg per person per day, respectively. The theoretical maximum daily intake (TMDI) values for carbendazim and diethofencarb were 1.5 and 0.5 mg/day, respectively. The dietary exposure was lower than the MPI, which indicates the harvested tomato samples under the experimental conditions (open field) are safe for human consumption at the recommended high dosage of the wettable powder.

  18. Influence of simulated Quinclorac drift on the accumulation and movement of herbicide in tomato (Lycopersicon esculentum) plants.

    Science.gov (United States)

    Lovelace, Michael L; Hoagland, Robert E; Talbert, Ronald E; Scherder, Eric F

    2009-07-22

    Quinclorac (3,7-dichloro-8-quinolinecarboxylic acid) is a herbicide commonly used in rice, and its drift has been suspected of causing injury to off-target tomato fields throughout Arkansas. Studies were conducted to evaluate the effects of single and multiple simulated quinclorac drift applications on tomato plant growth and development. Residues extracted from tomato plants treated with 0.42 g of ai ha(-1) were below the detection limit of liquid chromatography-double mass spectrometry (LC-MS/MS) analysis. Quinclorac residue levels and half-lives in tomato tissue increased as the application rate and number of applications increased. From 3 to 72 h after (14)C-quinclorac treatment of plants, most of the absorbed (14)C was retained in the treated leaf, and translocations of (14)C out of the treated leaf of vegetative and flowering tomato plant tissues were similar. Of the (14)C that translocated out of the treated leaf, the greatest movement was acropetally. The flower cluster contained 1% of the total absorbed (14)C, which suggests the potential for quinclorac translocation into tomato fruit. More extensive research will be required to understand the impact that quinclorac may have on tomato production in the area.

  19. Immobilization of tomato (Lycopersicon esculentum) pectinmethylesterase in calcium alginate beads and its application in fruit juice clarification.

    Science.gov (United States)

    Bogra, Pushpa; Kumar, Ashwani; Kuhar, Kalika; Panwar, Surbhi; Singh, Randhir

    2013-11-01

    Clarity of fruit juices is desirable to maintain an aesthetically pleasing quality and international standards. The most commonly used enzymes in juice industries are pectinases. A partially-purified pectinmethylesterase from tomato was entrapped in calcium alginate beads and used for juice clarification. The activity yield was maximum at 1 % (w/v) CaCl2 and 2.5 % (w/v) alginate. The immobilized enzyme retained ~55 % of its initial activity (5.7 × 10(-2) units) after more than ten successive batch reactions. The Km, pH and temperature optima were increased after immobilization. The most effective clarification of fruit juice (%T620 ~60 %) by the immobilized enzyme was at 4 °C with a holding time of 20 min. The viscosity dropped by 56 % and the filterability increased by 260 %. The juice remains clear after 2 months of storage at 4 °C.

  20. Characterization of culturable bacteria isolated from hot springs for plant growth promoting traits and effect on tomato (Lycopersicon esculentum) seedling.

    Science.gov (United States)

    Patel, Kinjal Samir; Naik, Jinal Hardik; Chaudhari, Sejal; Amaresan, Natarajan

    2017-03-23

    To elucidate the functional diversity of hot spring bacteria, 123 bacteria were isolated and screened for evaluating their multifunctional plant growth promoting (PGP) properties. The antagonistic activity against different phytopathogens showed the presence of a high amount of biocontrol bacteria in the hot springs. During screening for PGP properties, 61.0% isolates showed production of indole acetic acid and 23.6% showed inorganic phosphate solubilization qualitatively. For production of extracellular enzymes, it was found that 61.0% isolates produced lipase, 56.9% produced protease, and 43.9% produced cellulase. In extreme properties, half of the isolates showed tolerance to 5% NaCl (w/v) and 48.8% isolates survived heat shock at 70°C. The identification of 12 multipotential bacteria based on 16S rRNA gene sequencing revealed that the bacteria belonged to Aneurinibacillus aneurinilyticus and Bacillus spp. Bacterization of tomato seeds showed that the hot spring bacteria promoted shoot height, fresh shoot weight, root length, and fresh root weight of tomato seedlings, with values ranging from 3.12% to 74.37%, 33.33% to 350.0%, 16.06% to 130.41%, and 36.36% to 318.18%, respectively, over the control. This research shows that multifunctional bacteria could be isolated from the hot springs. The outcome of this research may have a potential effect on crop production methodologies used in saline and arid environments.

  1. Effective removal of tetracycline from aqueous solution using activated carbon prepared from tomato (Lycopersicon esculentum Mill.) industrial processing waste.

    Science.gov (United States)

    Sayğılı, Hasan; Güzel, Fuat

    2016-09-01

    Activated carbon (TAC) prepared under optimized conditions with ZnCl2 activation from a new precursor; tomato industrial processing waste (TW), was applied as an adsorbent to remove tetracycline (TC) from aqueous solution. The factors (TAC dosage, initial TC concentration, contact time, ionic strength and solution temperature) affecting the adsorption process were examined at natural pH (5.7) of TAC-TC system in aqueous solution. Kinetic data was found to be best complied by the pseudo-second order model. The isotherm analysis indicated that the equilibrium data could be represented by the Langmuir model. The maximum adsorption capacity was identified as 500.0mgg(-1) at 308K.

  2. Genetic and physical analysis of a YAC contig spanning the fungal disease resistance locus Asc of tomato (Lycopersicon esculentum)

    NARCIS (Netherlands)

    Mesbah, L.A.; Kneppers, T.J.A.; Takken, F.L.W.; Laurent, P.; Hille, J.; Nijkamp, H.J.J.

    1998-01-01

    The Alternaria stem canker disease of tomato is caused by the necrotrophic fungal pathogen Alternaria alternata f. sp. lycopersici (AAL). The fungus produces AAL toxins that kill the plant tissue. Resistance to the fungus segregates as a single locus, called Asc, and has been genetically mapped on c

  3. Essential Oils as Biocides for the Control of Fungal Infections and Devastating Pest (Tuta absoluta) of Tomato (Lycopersicon esculentum Mill.).

    Science.gov (United States)

    Bouayad Alam, Samira; Dib, Mohammed El Amine; Djabou, Nassim; Tabti, Boufeldja; Gaouar Benyelles, Nassira; Costa, Jean; Muselli, Alain

    2017-07-01

    Thymus capitatus and Tetraclinis articulata essential oils as well their major components (carvacrol and α-pinene) were evaluated for their antifungal and insecticidal activities. Both oils showed good in vitro antifungal activity against Fusarium oxysporum, Aspergillus niger, Penicillium sp., Alternaria alternata, and Botrytis cinerea, the fungi causing tomato rot. In vivo results indicate the efficacies of both essential oils and carvacrol of reduce postharvest fungal pathogens, such as B. cinerea and Al. alternata that are responsible of black and gray rot of tomato fruit. Disease incidence of Al. alternata and B. cinerea decreased on average from 55% to 80% with essential oil of Th. capitatus and pure carcvacrol, while Te. articulata essential oil exhibited inhibition of fungal growth of 55% and 25% against Al. alternata and B. cinerea, respectively, with concentration of 0.4 μl/l air. The insecticidal activity of Th. capitatus and Te. articulata essential oils exhibited also a good insecticidal activity. At the concentration of 0.2 μl/ml air, the oils caused mortality over 80% for all larval stages of Tuta absoluta and 100% mortality for the first-instar after 1.5 h only of exposure. α-Pinene presented lower insecticidal and antifungal activities compared to essential oils of Th. capitatus, Te. articulata and pure carvacrol. Thus, these essential oils can be used as a potential source to develop control agents to manage some of the main pests and fungal diseases of tomato crops. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

  4. Evaluation of Low Pressure Fogging System for Improving Crop Yield of Tomato (Lycopersicon esculentum Mill.: Grown under Heat Stress Conditions

    Directory of Open Access Journals (Sweden)

    Kobi Shilo

    2013-06-01

    Full Text Available In Mediterranean regions, many tomato plants are grown throughout the hot summer period (May–September in sheltered cultivation, mainly for plant protection reasons. Most of the shelters that are used are low cost net houses covered with 50 mesh insect proof net. In most cases these net houses have a flat roof and no ventilation or climate control measures. This insufficient ventilation during the hot summer leads to above optimal air temperatures and causes moderate heat stress inside the shelters, which leads to yield reduction. The aim of this study was to evaluate the ability of a simple and inexpensive low pressure fogging system installed in a naturally ventilated net house to lower temperatures and improve the yield during the summer. The study showed that in areas where relative air humidity (RH during the daytime is less than 60%, tomato plants improved their performance when grown through the summer in net houses under moderate heat stress. Under fogging conditions pollen grain viability and fruit set were significantly improved. This improvement influenced the yield picked during September (104–136 DAP. However, total seasonal yield was not affected by the fogging treatment.

  5. Salt tolerance in Lycopersicon species. IV. Efficiency of marker-assisted selection for salt tolerance improvement.

    Science.gov (United States)

    Monforte, A J; Asíns, M J; Carbonell, E A

    1996-10-01

    The usefulness of marker-assisted selection (MAS) to develop salt-tolerant breeding lines from a F2 derived from L. esculentum x L. pimpinellifolium has been studied. Interval mapping methodology of quantitative trait locus (QTL) analysis was used to locate more precisely previously detected salt tolerance QTLs. A new QTL for total fruit weight under salinity (TW) near TG24 was detected. Most of the detected QTLs [3 for TW, 5 for fruit number, (FN) and 4 for fruit weight (FW)] had low R (2) values, except the FW QTL in the TG180-TG48 interval, which explains 36.6% of the total variance. Dominant and overdominant effects were detected at the QTLs for TW, whereas gene effects at the QTLs for FJV and FW ranged from additive to partial dominance. Phenotypic selection of F2 familes and marker-assisted selection of F3 families were carried out. Yield under salinity decreased in the F2 generation. F3 means were similar to those of the F1 as a consequence of phentoypic selection. The most important selection response for every trait was obtained from the F3 to F4 where MAS was applied. While F3 variation was mainly due to the within-family component, in the F4 the FN and FW between-family component was larger than the within-family one, indicating an efficient compartmentalization and fixation of QTLs into the F4 families. Comparison of the yield of these families under control versus saline conditions showed that fruit weight is a key trait to success in tomato salt-tolerance improvement using wild Lycopersicon germplasm. The QTLs we have detected under salinity seem to be also working under control conditions, although the interaction family x treatment was significant for TW, thereby explaining the fact that the selected families responded differently to salinity.

  6. Induction of streptomycin resistance in the wild tomato Lycopersicon peruvianum

    NARCIS (Netherlands)

    Jansen, C.E.; Snel, E.A.M.; Akerboom, M.J.E.; Nijkamp, H.J.J.; Hille, J.

    1990-01-01

    A protoplast mutagenesis and cell selection system was used for the isolation of streptomycin resistant Lycopersicon peruvianum colonies. Protoplasts were treated with the mutagen N-nitroso-methylurea and could be regenerated into fertile plants, carrying the streptomycin resistant character. Severa

  7. Genetic differences in growth within and between Lycopersicon species

    NARCIS (Netherlands)

    Lindhout, Pim; Pet, Gerard; Jansen, Ritsert; Jansen, Hans

    1991-01-01

    Growth analyses were carried out on 88 accessions of five Lycopersicon species. Experiments were conducted in a climate room at 19/14°C day/night temperature which was irradiated at 20 W/m2 for eight hours per day. Large differences in plant weights between wild species and the cultivated tomato wer

  8. Long-life tomato cultivars growing under the hydroponic Nutrient Film Technique

    Directory of Open Access Journals (Sweden)

    Gualberto Ronan

    2002-01-01

    Full Text Available Farmers are interested in hydroponic tomato cultivation because of the high demand of high quality products by the consumers. Three trials here carried out under greenhouse conditions in Marília, SP, Brazil, using the hydroponic nutrient film technique, in order to evaluate yield and quality of long-life salad tomato (Lycopersicon esculentum varieties, during 1998 and 1999. Four varieties (Carmen, Diva, Graziela, and Vita were tested using a randomized block design, with six replications. The variety x crop season interaction effect was significant, meaning that varieties have distinct performances during the different cropping seasons. The Vita variety presented commercial valid yield, with mean fruit weights higher than the other varieties.

  9. Allergenicity assessment of genetically modified cucumber mosaic virus (CMV) resistant tomato (Solanum lycopersicon).

    Science.gov (United States)

    Lin, Chih-Hui; Sheu, Fuu; Lin, Hsin-Tang; Pan, Tzu-Ming

    2010-02-24

    Cucumber mosaic virus (CMV) has been identified as the causal agent of several disease epidemics in most countries of the world. Insect-mediated virus diseases, such as those caused by CMV, caused remarkable loss of tomato (Solanum lycopersicon) production in Taiwan. With expression of the CMV coat protein gene (Cmvcp) in a local popular tomato cultivar L4783, transgenic tomato line R8 has showed consistent CMV resistance through T(0) to T(8). In this report, the allergenicity of the CMV coat protein (CMV cp) expressed in transgenic tomato R8 was assessed by investigation of the expression of the transgene source of protein, sequence similarity with known allergens, and resistance to pepsin hydrolysis. There is no known account for either the CMV or its coat protein being an allergen. The result of a bioinformatic search also showed no significant homology between CMV cp and any known allergen. The pepsin-susceptible property of recombinant CMV cp was revealed by a simulated gastric fluid (SGF) assay. Following the most recent FAO/WHO decision tree, all results have indicated that CMV cp was a protein with low possibility to be an allergen and the transgenic tomato R8 should be considered as safe as its host.

  10. Lipid Profile in Different Parts of Edible Jellyfish Rhopilema esculentum.

    Science.gov (United States)

    Zhu, Si; Ye, Mengwei; Xu, Jilin; Guo, Chunyang; Zheng, Huakun; Hu, Jiabao; Chen, Juanjuan; Wang, Yajun; Xu, Shanliang; Yan, Xiaojun

    2015-09-23

    Jellyfish Rhopilema esculentum has been exploited commercially as a delicious food for a long time. Although the edible and medicinal values of R. esculentum have gained extensive attention, the effects of lipids on its nutritional value have rarely been reported. In the present of study, the lipid profile including lipid classes, fatty acyl compositions, and fatty acid (FA) positions in lipids from different parts (oral arms, umbrella, and mouth stalk) of R. esculentum was explored by ultraperformance liquid chromatography--electrospray ionization--quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS). More than 87 species from 10 major lipid classes including phosphatidylcholine (PC), lysophosphatidylcholine (LPC), phosphatidylethanolamine (PE), lysophosphatidylethanolamine (LPE), phosphatidylinositol (PI), lysophosphatidylinositol (LPI), phosphatidylserine (PS), ceramide (Cer), ceramide 2-aminoethylphosphonate (CAEP), and triacylglycerol (TAG) were separated and characterized. Semiquantification of individual lipid species in different parts of R. esculentum was also conducted. Results showed that glycerophospholipids (GPLs) enriched in highly unsaturated fatty acids (HUFAs) were the major compenents in all parts of R. esculentum, which accounted for 54-63% of total lipids (TLs). Considering the high level of GPLs and the FA compositions in GPLs, jellyfish R. esculentum might have great potential as a health-promoting food for humans and as a growth-promoting diet for some commercial fish and crustaceans. Meanwhile, LPC, LPE, and LPI showed high levels in oral arms when compared with umbrella and mouth stalk, which may be due to the high proportion of phospholipase A2 (PLA2) in oral arms. Moreover, a high CAEP level was detected in oral arms, which may render cell membranes with resistance to chemical hydrolysis by PLA2. The relatively low TAG content could be associated with specific functions of oral arms.

  11. Régénération in vitro de plantes de tomate Lycopersicon esculenteum Mill. adaptées au NaCl

    Directory of Open Access Journals (Sweden)

    Abir, M.

    2006-01-01

    Full Text Available In vitro Regeneration of NaCl-adapted Tomato Plants (Lycopersicon esculenteum Mill.. In vitro growth seedlings of tomato (Lycopersicon esculentum Mill. Var. Sabra were used as source of explants: internode segment and leaf fragment. These explants were cultivated into a MS-based medium containing 0.2 mg.l-1ANA and 2 mg.l-1 BAP, supplemented or not with NaCl at 3 and 6 g.l-1 in order to regenerate salt-tolerant plants. Results show that internode's segment and leaf fragment formed callus both in the control and in the saline media (3 and 6 g.l-1. However NaCl affected calli growth and mineral uptake. The callus growth was stimulated by NaCl at 3 g.I-1, but reduced at 6 g.I-1. Na+ and Cl- content of calli increased in saline media with respect to the control. K+ content decreased slightly with salinity, but Ca2+ content did not change. Leaf callus showed higher K+ and Ca2+ contents both in the control and saline media and were more salt tolerant than internodes callus. Latest ones regenerated shoots only on control medium. Leaf callus adapted to 3 g.l-1 NaCl were able to regenerate shoots, but with high percentage of hyperhydricity. Regenerated plants were rooted and acclimatized successfully and they tolerated 3 g.l-1 NaCl added to irrigation solution, when cultivated on peat under glasshouse.

  12. CULTIVAR RELEASE - BRS Saturno – triticale cultivar

    Directory of Open Access Journals (Sweden)

    Alfredo do Nascimento Junior

    2011-01-01

    Full Text Available The triticale cultivar BRS Saturno was developed by Embrapa (Brazilian Agricultural Research Corporation inpartnership with the Fundação Pró-Sementes de Apoio à Pesquisa. It is the result of a cross between the genotypes PFT 512 and CEP28 – Guará, adapted to the environmental conditions of cultivation in southern Brazil.

  13. Exploring genetic variation in the tomato (Solanum section Lycopersicon) clade by whole-genome sequencing.

    Science.gov (United States)

    Aflitos, Saulo; Schijlen, Elio; de Jong, Hans; de Ridder, Dick; Smit, Sandra; Finkers, Richard; Wang, Jun; Zhang, Gengyun; Li, Ning; Mao, Likai; Bakker, Freek; Dirks, Rob; Breit, Timo; Gravendeel, Barbara; Huits, Henk; Struss, Darush; Swanson-Wagner, Ruth; van Leeuwen, Hans; van Ham, Roeland C H J; Fito, Laia; Guignier, Laëtitia; Sevilla, Myrna; Ellul, Philippe; Ganko, Eric; Kapur, Arvind; Reclus, Emannuel; de Geus, Bernard; van de Geest, Henri; Te Lintel Hekkert, Bas; van Haarst, Jan; Smits, Lars; Koops, Andries; Sanchez-Perez, Gabino; van Heusden, Adriaan W; Visser, Richard; Quan, Zhiwu; Min, Jiumeng; Liao, Li; Wang, Xiaoli; Wang, Guangbiao; Yue, Zhen; Yang, Xinhua; Xu, Na; Schranz, Eric; Smets, Erik; Vos, Rutger; Rauwerda, Johan; Ursem, Remco; Schuit, Cees; Kerns, Mike; van den Berg, Jan; Vriezen, Wim; Janssen, Antoine; Datema, Erwin; Jahrman, Torben; Moquet, Frederic; Bonnet, Julien; Peters, Sander

    2014-10-01

    We explored genetic variation by sequencing a selection of 84 tomato accessions and related wild species representative of the Lycopersicon, Arcanum, Eriopersicon and Neolycopersicon groups, which has yielded a huge amount of precious data on sequence diversity in the tomato clade. Three new reference genomes were reconstructed to support our comparative genome analyses. Comparative sequence alignment revealed group-, species- and accession-specific polymorphisms, explaining characteristic fruit traits and growth habits in the various cultivars. Using gene models from the annotated Heinz 1706 reference genome, we observed differences in the ratio between non-synonymous and synonymous SNPs (dN/dS) in fruit diversification and plant growth genes compared to a random set of genes, indicating positive selection and differences in selection pressure between crop accessions and wild species. In wild species, the number of single-nucleotide polymorphisms (SNPs) exceeds 10 million, i.e. 20-fold higher than found in most of the crop accessions, indicating dramatic genetic erosion of crop and heirloom tomatoes. In addition, the highest levels of heterozygosity were found for allogamous self-incompatible wild species, while facultative and autogamous self-compatible species display a lower heterozygosity level. Using whole-genome SNP information for maximum-likelihood analysis, we achieved complete tree resolution, whereas maximum-likelihood trees based on SNPs from ten fruit and growth genes show incomplete resolution for the crop accessions, partly due to the effect of heterozygous SNPs. Finally, results suggest that phylogenetic relationships are correlated with habitat, indicating the occurrence of geographical races within these groups, which is of practical importance for Solanum genome evolution studies.

  14. Cell-wall-associated endo-B-mannanase increases in the skin and outer pericarp of tomato fruits during ripening

    NARCIS (Netherlands)

    Bewley, J.D.; Banik, M.; Bourgault, R.; Fuertado, A.; Toorop, P.; Hilhorst, H.W.M.

    2000-01-01

    Activity of endo-ß-mannanase increases during ripening of tomato (Lycopersicon esculentum Mill.) fruit of the cultivar Trust. ß-Mannoside mannohydrolase is also present during ripening, but its pattern of activity is different from that of endo-ß-mannanase. The increase in endo-ß-mannanase activity

  15. GenBank blastx search result: AK243656 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK243656 J100088L22 AF203480.1 AF203480 Lycopersicon esculentum cultivar R11-12 (35...S::Pto in Rio Grande x Money Maker)' phosphoenolpyruvate carboxylase kinase mRNA, partial cds. PLN 2e-53 1 ...

  16. GenBank blastx search result: AK243187 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK243187 J100039E11 AF203480.1 AF203480 Lycopersicon esculentum cultivar R11-12 (35...S::Pto in Rio Grande x Money Maker)' phosphoenolpyruvate carboxylase kinase mRNA, partial cds. PLN 1e-41 1 ...

  17. GenBank blastx search result: AK241003 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK241003 J065053E13 AF203480.1 AF203480 Lycopersicon esculentum cultivar R11-12 (35...S::Pto in Rio Grande x Money Maker)' phosphoenolpyruvate carboxylase kinase mRNA, partial cds. PLN 1e-14 1 ...

  18. Influence of sub-optimal temperature on tomato growth and yield : a review

    NARCIS (Netherlands)

    Ploeg, van der A.; Heuvelink, E.

    2005-01-01

    The effects of temperature on growth, development and yield of tomato (Lycopersicon esculentum) are reviewed with special emphasis on cultivar differences. The focus is on sub-optimal temperatures, above the level where chilling injury occurs. Temperature has a large effect on all aspects of develop

  19. Spatial distribution of bumblebees foraging on two cultivars of tomato in a commercial greenhouse.

    Science.gov (United States)

    Lefebvre, Diane; Pierre, Jacqueline

    2006-10-01

    The spatial distribution of foraging bumblebees, Bombus terrestris L. (Hymenoptera: Apidae), was studied in a greenhouse planted with two cultivars of tomato, Lycopersicum esculentum Mill. (Solanaceae), in two patches. In both patches, bumblebee densities per square meter were measured on plots, and the results showed that their densities were nearly similar. The densities of available flowers, their pollen production, and availability also were measured. Our results showed that, although the cultivars greatly differed in flower density, flower morphology, and pollen production, their pollen availability (i.e., pollen actually collected by bumblebees per square meter) was approximately the same. Therefore, the mean quantities of pollen collected per bumblebee were similar in each patch. Knowing that bumblebees do not visit different varieties randomly, our results suggest that the major factor affecting the bumblebee distribution among patches was the density of available resource. Results are discussed both from an applied point of view and in relation to the assumptions of the ideal free distribution theory.

  20. Processamento mínimo de tomates (Lycopersicum esculentum Mill) :

    OpenAIRE

    Rosa, Mônica de Caldas

    1999-01-01

    Dissertação (Mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias. O perfil atual de consumidores tem impulsionado o incremento de vegetais minimamente processados, considerados produtos de conveniência, prontos para consumo, que mantém as características próximas ao do similar in natura. Com os objetivos de minimizar os custos de produção e manter a qualidade destes produtos, foi realizado um estudo de processamento mínimo de tomates (Lycopersicum esculentum Mil...

  1. Evaluation of the Effect of Ecologic on Root Knot Nematode, Meloidogyne incognita, and Tomato Plant, Lycopersicon esculenum

    Directory of Open Access Journals (Sweden)

    Gary W. Lawrence

    2008-06-01

    Full Text Available Nonchemical methods and strategies for nematode management including cultural methods and engineered measures have been recommended as an alternative to methyl bromide (a major soil fumigant, due to its role in the depletion of the ozone layer. Hence, an international agreement has recently been reached calling for its reduced consumption and complete phasing out. This present research evaluates the potential of Ecologic, a biological, marine shell meal chitin material, as a soil amendment management agent for root knot nematode, Meloidogyne incognita, control, and its effect on the growth of Floradel tomato plant, Lycopersicon esculentum. To accomplish this goal, studies were conducted during which, experimental pots were set up in greenhouse environments using sterilized soil inoculated with 5,000 root-knot eggs per 1500 g soil. There were 4 treatments and 5 replications. Treatments were: No chitin; 50 g chitin; 100 g chitin; and 200 g chitin. A two-week wait period following Ecologic amendment preceded Floradel tomato planting to allow breakdown of the chitin material into the soil. Fresh and dry weights of shoot and root materials were taken as growth end-points. A statistically significant difference (p ≤ 0.05 was obtained with regard to the growth rate of L. esculentum at 100 g chitin treatment compared to the control with no chitin. Mean fresh weights of Floradel tomato were 78.0 ± 22.3g, 81.0 ± 20.3g, 109.0 ± 25.4g and 102.0 ± 33.3g at 0, 50, 100 and 200g chitin, respectively. The analysis of root knot nematode concentrations indicated a substantial effect on reproduction rate associated with chitin amendment. Study results showed a significant decrease in both root knot nematode eggs and juveniles (J2 at 100g and 200g Ecologic chitin levels, however, an increase in nematode concentrations was recorded at the 50g Ecologic chitin level (p ≤ 0.05. The mean amounts of J2 population, as

  2. Determination of heavy metals in soil and different parts of Diplazium esculentum (medicinal fern)

    Science.gov (United States)

    Jasim, Hind S.; Idris, Mushrifah; Abdullah, Aminah; Kadhum, A. A. H.

    2014-09-01

    Diplazium esculentum is a widely used medicinal fern in Malaysia and other regions worldwide. Heavy metals in plants should be determined because prolonged human intake of toxic trace elements, even at low doses, results in organ malfunction and causes chronic toxicity. Hence, substantial information should be obtained from plants that grow on soils containing high concentrations of heavy metals. This study aimed to determine the physicochemical characteristics of soil and heavy metal concentrations (Pb, Cr, Mn, Cu, and Zn) in different parts of D. esculentum and soil, which were collected from the fern garden of Universiti Kebangsaan Malaysia. Results showed that heavy metals were highly accumulated in D. esculentum roots.

  3. Pessegueiro: cultivar BRS kampai

    Directory of Open Access Journals (Sweden)

    Maria do Carmo Bassols Raseira

    2010-12-01

    Full Text Available RESUMO O programa de Melhoramento Genético de Pessegueiros da Embrapa Clima Temperado tem, entre seus objetivos, a obtenção de cultivares produtoras de frutas para consumo in natura, com características que satisfaçam às exigências dos consumidores. Alguns dos grandes centros consumidores, como é o caso de São Paulo e Curitiba, preferem pêssegos de polpa branca e sabor doce. A cultivar BRS Kampai, obtida de um cruzamento entre 'Chimarrita' e 'Flordaprince', alia a baixa necessidade em frio, o que é uma vantagem em regiões subtropicais, à boa aparência, com sabor superior a qualquer um dos parentais. A colheita dos frutos desta cultivar inicia-se geralmente, em meados de novembro, em Pelotas-RS (em Atibaia, São Paulo, inicia-se na segunda quinzena de outubro, poucos dias antes das cultivares Rubimel (polpa amarela e Premier (polpa branca, sendo ótima substituta para esta última.

  4. EVALUACIÓN Y SELECCIÓN DE NUEVAS LÍNEAS DE TOMATE (Lycopersicon esculentum Mill. CON ALTOS RENDIMIENTOS Y FRUTOS DE ALTA CALIDAD

    Directory of Open Access Journals (Sweden)

    C. Moya

    2005-01-01

    Full Text Available El presente trabajo se llevó a cabo en el Instituto Nacional de Ciencias Agrícolas (INCA, con el objetivo de seleccionar y evaluar nuevas líneas y variedades de tomate de crecimiento indeterminado, obtenidas en el programa de mejora del instituto e introducidas recientemente en el país, las cuales fueran capaces de aportar altos rendimientos y frutos de alta calidad, en las nuevas tecnologías de cultivo utilizadas en la producción comercial. El trabajo se desarrolló durante el período comprendido entre las campañas de invierno 1999- 2000 y 2002-2003, durante el cual se evaluaron 14 combinaciones F1. Se obtuvo la generación F2 y se procedió en cada una de ellas a la selección de las plantas que cumplían con los criterios de selección establecidos, utilizándose un fruto de cada planta, procedimiento que se continuó hasta la generación F5, con la comparación de seis de ellas con el híbrido comercial FA-180 y las variedades Floradel, Saint Pierre y Virginia. El sustrato utilizado se conformó con una mezcla de suelo Ferralítico Rojo y materia orgánica en proporción de 2:1. Se realizaron evaluaciones morfológicas y fenológicas así como determinaciones de rendimiento y sus principales componentes. El diseño experimental utilizado fue un completamente aleatorizado, sembrándose 10 plantas por variedad a una distancia de siembra de 70 cm entre hileras y 25 cm entre plantas. Los resultados experimentales resultantes permiten recomendar las líneas 25, 31, 26 y 24, y la nueva variedad Virginia para pruebas a mayor escala y por su empleo como progenitores en futuros programas de mejora.

  5. The Effect of MethyI Jasmonate on Ethylene Production, ACC Oxidase Activity and Carbon Dioxide Evolution in the Yellowish-Tangerine Tomato Fruits (Lycopersicon esculentum Mill.

    Directory of Open Access Journals (Sweden)

    Janusz Czapski

    2013-12-01

    Full Text Available The yellowish-tangerine tomato (cv. Bursztyn in the green, light yellow and yellow stages of ripening were treated with 0.1% and 1.0% of methyl jasmonate (JA-Me in lanolin paste and kept for several days and then they were evaluated for production of ethylene, ACC oxidase activity and CO2 evolution. Production of endogenous ethylene in mature green fruits was low and increased during ripening. JA-Me stimulated ethylene production and ACC oxidase activity in all investigated stages of fruit ripening. Slices excised from mature green fruits produced highest amount of carbon dioxide as compared to more advanced stages of ripening. JA-Me in O,1 % and 1,0% concentrations increased significantly CO2 evolution in green fruits, while in light yellow and yellow fruits only higher concentration of JA-Me stimulated carbon dioxide production.

  6. Cloning and Expression of an Alternative Oxidase Gene from Lycopersicon esculentum%番茄交替氧化酶基因的克隆和表达

    Institute of Scientific and Technical Information of China (English)

    宋从凤; Broth wayne; 王金生; 胡晋生

    2004-01-01

    A full-length cDNA gene (LeAoxlau) was isolated from a cDNA library made from ripening fruit probing with alternative oxidase (AOX) gene fragments, obtained by degenerate primer PCR. Sequence analysis showed that LeAoxlau was 1 418 bp long and contained a 1 077-bp open reading frame encoding a about 40 kD precursor protein which is processed to a mature protein of 32 kD. Southern blot analysis suggested LeAoxlau is present as a single copy in the genome of tomato. RT-PCR analysis indicated LeAoxlau was expressed in roots, stems, leaves and cotyledons of tomato plants. A recombinant construct containing the open reading frame sequence of the LeAoxlau was transformed into Escherichia coli to express the alternative oxidase precursor protein.%利用简并PCR扩增产物做探针筛选番茄cDNA基因文库获得一个全长交替氧化酶cDNA基因LeAoxlau.经序列分析得出,该基因全长1 418bp,编码区序列长1 077 bp,编码约40 kD的前体蛋白.该蛋白在转运到线粒体时被加工成32kD的成熟蛋白.Southern印迹杂交分析结果显示该基因以单拷贝形式存在于番茄的基因组中RT-PCR显示,该基因在在番茄植株的根、茎、叶和子叶中表达.重组表达实验表明该基因能在大肠杆菌中表达.

  7. Análisis de la acumulación de azúcares en pericarpios de dos genotipos silvestres de jitomate (lycopersicon esculentum)

    OpenAIRE

    Eleazar Martínez Barajas

    2003-01-01

    Para determinar las bases bioquímicas de la acumulación de azúcares en frutos maduros de jitomates silvestres, se analizaron dos genotipos que acumulan hexosas y que presentan grandes diferencias en el contenido de azúcares. La acumulación de hexosas se inició al principio del período de maduración. Los fotosintatos generados por la planta durante el período de maduración fueron muy importantes para determinar los niveles de azúcares en los frutos maduros. El pericarpio del genotipo que acumu...

  8. Application of a LED-based reflectance sensor for the assessing in situ the lycopene content of tomatoes (Lycopersicon esculentum Mill.)

    Science.gov (United States)

    Mignani, Anna G.; Ciaccheri, Leonardo; Mencaglia, Andrea A.; Tuccio, Lorenza; Agati, Giovanni

    2015-05-01

    Nondestructive in situ determination of the antioxidant lycopene of fresh tomato fruits is of large interest for the growers, willing to optimize the harvest time for high quality products. For this, we developed a portable LED-based colorimeter which was able to measure reflectance spectra of whole tomatoes in the 400-750 nm range. The tomato skins from the same samples were then frozen in liquid nitrogen, extracted with an acetone/ethanol/hexane mixture and analyzed by means of a spectrophotometer for their lycopene content. Concentration of lycopene was varying between 70 and 550 mg/Kg fresh weight skin. Partial Least Square regression was used to correlate spectral data to the tomato lycopene content. The multivariate processing of the reflectance data showed that lycopene content could be nicely predicted with a coefficient of determination R2=0.945 and a root mean square error of cross-validation RMSECV=57 mg/Kg skin fresh weight. These results suggest that portable, low-cost and compact LED-based sensors appear to be promising instruments for the nondestructive assessment of tomato lycopene even in the field.

  9. Efectividad de la combinación biofertilizantes-análogo de brasinoesteroides en la nutrición del tomate (Lycopersicon esculentum Mill

    Directory of Open Access Journals (Sweden)

    Elein Terry

    2001-01-01

    Full Text Available Con el objetivo de estudiar el efecto producidopor la combinación de biofertilizantes (AZOFERT y ECOMIC–análogo de brasinoesteroides (BIOBRAS-16 sobre el crecimiento, desarrollo y rendimiento del tomate, se llevó a cabo un experimento de campo en los años 1998 y 1999, sobre un suelo Ferralítico Rojo compactado. Se realizaron combinaciones simples y mixtas de los biofertilizantes más el BIOBRAS-16, obteniéndose como resultados que la coinoculación AZOFERT-ECOMIC más la aplicación del BIOBRAS-16 al inicio de la floración del cultivo del tomate fue la combinación que permitió incrementar el rendimiento del cultivo, además de permitir el ahorro de 60 kg.ha-1 de nitrógeno. Se logró una alta población de la rizobacteria en la rizosfera del cultivo así como un porcentaje de infección positivo del hongo micorrizógeno.

  10. Depth of dormancy in tomato (Lycopersicon esculentum Mill.) seeds is related to the progression of the cell cycle prior to the induction of dormancy

    NARCIS (Netherlands)

    Castro, de R.D.; Bino, R.J.; Jing, H.C.; Hilhorst, H.W.M.

    2001-01-01

    Cell cycle activities are initiated following imbibition of non-dormant seeds. However, it is not known whether cell cycle related events other than DNA replication also remain suppressed in imbibed dormant seeds. The objective of this study was to demonstrate that the transitions between the non-do

  11. Perfeccionamiento del sustrato en el modelo V.S.P. para la producción de plántulas de tomate Lycopersicon esculentum Mill

    OpenAIRE

    Gómez Zambrano Jairo; Bruzón C. Serapio F.

    1996-01-01

    To the sustrate (filter press cake of sugar cane and botton fly ash 3:1 v/v) poultry manure and lombricompost from cow dung at levels of 0,6 and 12% v/v were aditioned and soluble sources of Nitrogen (0,150, 300), phosphorus (0,100,200), iron (0,40,60) and copper (0,20,40 g/m3).  It were planted tomato seeds of Santa Clara variety in plastic cups. The experimental desing was one complete randomnized with eigh repetitions. After 35 days the high (cm), the breadt...

  12. Dynamic assessment of air temperature for tomato (Lycopersicon esculentum mill) cultivation in a naturally ventilated net-screen greenhouse under tropical lowlands climate

    NARCIS (Netherlands)

    Shamshiri, R.; Beveren, van P.; Che Man, H.; Zakaria, A.J.

    2017-01-01

    Net-screen covered greenhouses operating on natural ventilation are used as a sustainable approach for closed-field cultivation of fruits and vegetables and to eliminate insect passage and the subsequent production damage. The objective of this work was to develop a real-time assessment framework fo

  13. 番茄同源四倍体离体诱导研究%Study on in Viro Induction of Autotetraploid of Tomato(Lycopersicon esculentum Mlll.)

    Institute of Scientific and Technical Information of China (English)

    吴志刚; 刘丹赤; 宋明

    2012-01-01

    [Objective] Study on in viro induction of autotetraploid of tomato was conducted by using the conbation of the tissue culture technology and the colchicine treatment. [ Method] With the tomato variety Yufen 109 as the tested material, based on the establishment of the tomato plant efficient in viro regeneration system, taking the cotyledons callus with the green bud growing point as the induction materials, the in viro induction of autotetraploid of tomato was conducted reap. By means of mixed culture method and immersion method with the colchicine. [ Result] When taking the cotyledons callus with the green bud growing point as the induction materials and in viro induction of autotetraploid of tomato was adopted by the mixed culture method, the treatment with 0. 2% colchicines for 48 h could get highest induction rate, being 50% , while that by the immersion method got higher induction rate, but had a great harm on the explant. [ Conclusion ] The higher induction rate was obtained by using the mixed culture method, which provided a new way for the improvement of variety and innovation and abundance of germplasm resources in the tomato.%[目的]利用组织培养技术与秋水仙素相结合进行番茄四倍体的离体诱导研究.[方法]以番茄品种渝粉109为试验材料,在建立番茄植株高效离体再生体系的基础上,以带绿芽生长点的子叶愈伤组织为诱导材料,利用秋水仙素分别采用混培法和浸渍法进行番茄同源四倍体的离体诱导.[结果]以带绿芽生长点的子叶愈伤组织为诱导材料,番茄同源四倍体离体诱导采用混培法,0.2%的秋水仙素处理48h时获得了最高诱导率,为50.0%;浸渍法虽然诱导率较高,但对外植体的药害程度大.[结论]通过混培法获得了较高的诱导率,为番茄品种改良和种质资源的创新与丰富提供了一条新的途径.

  14. EFECTO DE LA COMPOSTA Y TÉ DE COMPOSTA EN EL CRECIMIENTO Y PRODUCCIÓN DE TOMATE (LYCOPERSICON ESCULENTUM MILL.) EN INVERNADERO

    OpenAIRE

    2015-01-01

    The effect of two organic fertilizers was evaluated within the development of this project, compost at concentrations of 3, 4, 5 and 6 tons per hectare and compost tea at concentrations of 10, 20, 30, 40 and 50 % v / v, for the production of tomato variety Hermosa saladette under greenhouse conditions. Treatments were divided into entirely randomized plots. The statistical analysis of the outcomes found significant differences in plant height variables and titratable acidity, while the perfor...

  15. Capítulo VIII: heredabilidad del rendimiento y sus componentes en tomate, Lycopersicon esculentum, Mill.; correlaciones genéticas y ambientales

    Directory of Open Access Journals (Sweden)

    Lobo Arias Mario

    1994-12-01

    Full Text Available

    El presente estudio se desarrolló con el fin de determinar la heredabilidad, en sentido amplio, del rendimiento y sus componentes en tomate y a la vez describir las relaciones genotípicas y ambientales para dichos componentes en una población constituida por tres líneas endocriadas (Ponderosa Red, Chonto y Red Cherry como progenitores y las F1 provenientes de los cruzamientos entre los progenitores en una sola dirección. La heredabilidad se estimó en base a los cuadrados medios esperados del análisis de varianza y las correlaciones genotípicas y ambientales en base a los cuadrados medios esperados del análisis de varianza y productos cruzados medios del análisis de covarianza. Para el rendimiento se estimó una heredabilidad de 79.3%, para el número total de frutos de 93.107%, para peso promedio de de fruto de 4.25%. Los coeficientes de correlación genotípicos estimados fueron: para número de frutos por planta y peso promedio de frutos de -0.985; para número de inflorescencias por planta y número de frutos por inflorescencia de 0,541; para número de lóculos por fruto y peso promedio de lóculos de 0.826. Los coeficientes de correlación ambiental estimados fueron: para número de frutos por planta y peso promedio de frutos de -0.512; para rendimiento y número de frutos por planta de -0.065; para rendimiento y peso promedio de frutos de 0.613; para número de inflorescencias y número de frutos por inflorescencias de -0.878; para número de lóculos por fruto y peso promedio de lóculos de -0.779. Lo anterior indica que el método de mejoramiento para rendimiento, dentro de la población estudiada, seria la selección, tomando como índice de selección apropiado el peso promedio de frutos pero sin descuidar el número de frutos. Se debe maximizar las condiciones favorables de suelo y manejo de cultivo para permitir una adecuada manifestación de los dos componentes primarios del rendimiento.

    This study intended to determine heritability, in the wide sense, of tomato yield and its components and their genotypic and environmental correlations in a population composed of three inbred lines (Ponderosa Red, Chonto y Red Cherry and the F1 from one direction crosses. Heritability was estimated from the expected mean squares and genotypic and environmental correlations were calculated from E.M.S. and cross products from the analysis of covariance. For total yield, number of fruits per plant and mean fruit weight calculated heritability were 79.3%, 93.107% and 94.25%, respectively. Genotypic correlation coefficients were: between number of fruits per plant and mean fruit weight -0.985; between number of clusters and fruits per cluster 0.541; between locules por fruit and mean weight of locule 0.826. Environmental correlations were: between number of fruits per plant and mean weight of fruits 0.512; between yield and number of fruits per plant 0.065; between yield and mean weight of fruits 0.613; between number of clusters and fruits per cluster 0.878; between number of locules per fruit and mean locule weight 0.779. The results indicate that the best method to improve yields would be a selection index considering mean weight of fruits and giving some consideration to number of fruits per plant. In order to let these characteristics to express themselves maximum soil and crop management should be provided.

  16. Different blue-light requirement for the accumulation of transcripts from nuclear genes for thylakoid proteins in Nicotiana tabacum and Lycopersicon esculentum.

    Science.gov (United States)

    Palomares, R; Herrmann, R G; Oelmüller, R

    1991-11-01

    We have isolated recombinant lambda gt11 phages which carry cDNA clones for the major light-harvesting chlorophyll a/b-binding proteins of photosystem I (LHCPI) and II (LHCPII), subunit II of photosystem I, a chlorophyll a/b-binding protein of photosystem II (CP24), the Rieske iron-sulphur protein of the cytochrome b6/f complex, and the 33, 23 and 16 kDa proteins of the water-oxidizing complex of photosystem II from Nicotiana tabacum. The nucleotide sequences of cDNA clones encoding the precursors for LHCPI and the FeS protein are presented. If tobacco or tomato seedlings, or seedlings of a phytochrome-deficient aurea mutant of tomato which lacks more than 95% of the phytochrome of the isogenic wild type, are kept in blue light, the transcript level of each of these genes is higher than in seedlings grown in red light suggesting the involvement of a blue-UVA-light photoreceptor. In the case of LHCPI, a 1 min blue-light pulse applied to red-light-grown seedlings is sufficient to increase the transcript levels to those present in blue-light-grown seedlings, whereas almost no increase is observed for transcripts encoding the FeS and 33 kDa proteins. If dark-grown tomato seedlings receive a single far-red-light pulse, significant stimulation is detected for LHCPI transcripts, whereas transcripts encoding the FeS and 33 kDa proteins are not stimulated. It is concluded that the lower light requirement for the increase in the LHCPI transcript level is not specific for one of the light-dependent signal transduction chains.

  17. Effects of 2,4-D and 4-CPA on yield and quality of the tomato, Lycopersicon esculentum Miller (Solanaceae)

    DEFF Research Database (Denmark)

    Gemici, Meliha; Türkyilmaz, B.; Tan, Kit

    2007-01-01

    economy. Our tests confirm that application of concentrations in excess of the recommended dosage produces deformed and inferior fruit, increases fruit number and incidence of parthenocarpy. The use of growth regulators led to an increase in the levels of the internal plant hormone indol-3 acetic acid......Synthetic plant growth substances augment plant growth when applied at specific concentrations; they are also reported as without causing significant increases in the amount of internal plant hormones. Exceeding recommended dosages, however, affects yield and quality adversely. An experiment......, to test the effects of high concentrations of two growth regulators 2,4- dichlorophenoxy acetic acid (2,4-D) and 4-chlorophenoxy acetic acid (4-CPA) on fruit development in the tomato plant, was carried out. This plant is grown on a large scale in Turkey, contributing to and supporting the national...

  18. Reúso del tezontle: efecto en sus características físicas y en la producción de tomate (Lycopersicon esculentum Mill

    Directory of Open Access Journals (Sweden)

    Eduardo Rodríguez Díaz

    2013-10-01

    Full Text Available Se estudiaron los cambios en las propiedades físicas del Tezontle por efecto de su reúso como sustrato para el cultivo de tomate (primer uso T0, primer reúso T1 y segundo reúso T2, así como la influencia de dichos cambios en la producción. La investigación se realizó en Tlajomulco, Jalisco bajo invernadero, en un sistema hidropónico, de agosto de 2009 a septiembre de 2010. La variedad de tomate fue la SUN 7705. El diseño experimental fue completamente al azar y se evaluaron las propiedades físicas del sustrato (granulometría, densidad aparente, retención de humedad y aireación al inicio y al final de cada ciclo de cultivo; asimismo, la redistribución del tamaño de partículas, así como algunas variables de respuesta del cultivo (peso de fruto, grados Brix, altura de planta y diámetro de tallo. Se observó un aumento en la capacidad de retención de humedad del tezontle de 43% en T0, 46% en T1 y 48% en T2, una disminución de la capacidad de aireación de 12% en T0, 9% en T1 y 8% en T2, debido a cambios en la distribución del tamaño de partícula por el reúso; dichos cambios no afectaron agronómicamente las variables de peso de fruto y grados Brix; las diferencias estadísticas, solo fueron significativas en altura de planta y diámetro de tallo. Los resultados obtenidos demostraron que el tezontle como sustrato puede utilizarse durante tres ciclos consecutivos de cultivo de tomate hidropónico sin demeritar su productividad, permitiendo un ahorro aproximado de ciento sesenta mil pesos por hectárea por cada reúso.

  19. Evaluation of salt tolerance in ectoine-transgenic tomato plants (Lycopersicon esculentum) in terms of photosynthesis, osmotic adjustment, and carbon partitioning.

    Science.gov (United States)

    Moghaieb, Reda E A; Nakamura, Akiko; Saneoka, Hirofumi; Fujita, Kounosuke

    2011-01-01

    Ectoine is a common compatible solute in halophilic bacteria. Its biosynthesis originates from L-aspartate β-semialdehyde and requires three enzymes: L-2, 4-diaminobutyric acid aminotransferase (gene: ect B), L-2,4-diaminobutyric acid acetyl transferase (gene: ect A) and L-ectoine synthase (gene: ect C). Genetically engineered tomato plants expressing the three H. elongata genes (ectA, ectB, and ectC) generated showed no phenotypic abnormality. Expression of the ectoine biosynthetic genes was detected in the T3 transgenic plants by Northern blot analysis. The ectoine accumulating T3 plants were evaluated for salt tolerance by examining their photosynthestic activity, osmotic adjustment and carbon partitioning. Nuclear magnetic resonance (NMR) detected the accumulation of ectoine. The concentration of ectoine increased with increasing salinity. The transgenic lines showed higher activities of peroxidase, while the malondialdehyde (MDA) concentration was decreased under salinity stress condition. In addition, preservation of higher rates of photosynthesis and turgor values as compared to control was evident. Within a week of ( 13) CO 2 feeding, salt application led to increases in the partitioning of ( 13) C into roots at the expense of ( 13) C in the other plant parts. These results suggest that under saline conditions ectoine synthesis is promoted in the roots of transgenic plants, leading to an acceleration of sink activity for photosynthate in the roots. Subsequently, root function such as water uptake is improved, compared with wild-type plants. In this way, the photosynthetic rate is increased through enhancement of cell membrane stability in oxidative conditions under salt stress.

  20. Etude du comportement des cultures de tomate (Lycopersicon esculentum Mill. et de piment (Capsicum annuum L. conduites en lignes simples et lignes jumelées sous serre

    Directory of Open Access Journals (Sweden)

    Boujelben, A.

    2004-01-01

    Full Text Available Behaviour Study of Tomato Lycopercicum esculuntum Mill. and Red Pepper Capsicum annuum L. Crops under Greenhouse Conditions Conducted in Single and Twinned Rows. Tomato (Lycopercicum esculuntum Mill. and red pepper (Capsicum annuum L. crops under greenhouse conditions and drip irrigation are widely developed in Tunisia. Although, it is known that drip irrigation permitted some water savings. Other cultural techniques such as single or twinned planting rows combined with drip irrigation system could improve the water management. In this aim we have conducted an experimental trial under greenhouse adopting drip irrigation with simple and double amounts of water and single and twinned planting rows. Some agronomic traits in the occurrence the height of plants, the fruit number and yield per plant, the precocity and the water efficiency were studied. The red pepper culture with a spacing of 80 cm between lines, gave the best results in the simple row and simple amount of irrigation treatment. On the other hand, tomato, in twinned rows (90 cm among rows with double amount of water gave the best reaps in yield and more precocious. Whereas the higher water irrigation efficiency was obtained for the treatment combining the twinned planting rows and simple amount of water.

  1. In vitro selection of NaCl-tolerant mutant of Lycopersicon esculentum Mill%栽培番茄耐盐突变体的离体筛选

    Institute of Scientific and Technical Information of China (English)

    陈火英; 张建华; 张晓宁

    2002-01-01

    栽培番茄品种"矮黄"的子叶作为外植体诱导愈伤组织,用NaCl进行直接高盐胁迫筛选.结果表明,225 mmol/LNaCl直接高盐胁迫获得了耐盐突变体,10株完整的再生耐盐植株,在150 mmol/LNaCl的盐胁迫下,成活率可达70%,而未经胁迫筛选过的原始株成活率则为零.其中,1株耐盐突变株能正常开花、结果.对其后代离体培养的茎尖和下胚轴外植体耐盐性进行比较,表明其后代仍然保持耐盐性.%To obtain salt-tolerant tomato plants, NaCl at a different level was added into the culture medium. Cotyledon-derived calli tolerant to 225mmol/L NaCl were selected in vitro and regenerated into plants. The 10 plants regenerated from salt-tolerant calli obtained by a direct use of 225mmol/L NaCl showed high tolerance when subjected to NaCl stress. Plants retained tolerance following transplant to containers filled with a mixture of peat and vermiculite (3∶1) and watered with 150 mmol/L NaCl. But only one salt-tolerant regenerants flowered and fruited, and transferred tolerance to their progeny following self-pollination.

  2. Ethylene emission and PR protein synthesis in ACC deaminase producing Methylobacterium spp. inoculated tomato plants (Lycopersicon esculentum Mill.) challenged with Ralstonia solanacearum under greenhouse conditions.

    Science.gov (United States)

    Yim, Woojong; Seshadri, Sundaram; Kim, Kiyoon; Lee, Gillseung; Sa, Tongmin

    2013-06-01

    Bacteria of genus Methylobacterium have been found to promote plant growth and regulate the level of ethylene in crop plants. This work is aimed to test the induction of defense responses in tomato against bacterial wilt by stress ethylene level reduction mediated by the ACC deaminase activity of Methylobacterium strains. Under greenhouse conditions, the disease index value in Methylobacterium sp. inoculated tomato plants was lower than control plants. Plants treated with Methylobacterium sp. challenge inoculated with Ralstonia solanacearum (RS) showed significantly reduced disease symptoms and lowered ethylene emission under greenhouse condition. The ACC and ACO (1-aminocyclopropane-1-carboxylate oxidase) accumulation in tomato leaves were significantly reduced with Methylobacterium strains inoculation. While ACC oxidase gene expression was found higher in plants treated with R. solanacearum than Methylobacterium sp. treatment, PR proteins related to induced systemic resistance like β-1,3-glucanase, PAL, PO and PPO were increased in Methylobacterium sp. inoculated plants. A significant increase in β-1,3-glucanase and PAL gene expression was found in all the Methylobacterium spp. treatments compared to the R. solanacearum treatment. This study confirms the activity of Methylobacterium sp. in increasing the defense enzymes by modulating the ethylene biosynthesis pathway and suggests the use of methylotrophic bacteria as potential biocontrol agents in tomato cultivation.

  3. Tomato (Lycopersicon esculentum) Supplementation Induces Changes in Cardiac miRNA Expression, Reduces Oxidative Stress and Left Ventricular Mass, and Improves Diastolic Function.

    Science.gov (United States)

    Pereira, Bruna L B; Arruda, Fernanda C O; Reis, Patrícia P; Felix, Tainara F; Santos, Priscila P; Rafacho, Bruna P; Gonçalves, Andrea F; Claro, Renan T; Azevedo, Paula S; Polegato, Bertha F; Okoshi, Katashi; Fernandes, Ana A H; Paiva, Sergio A R; Zornoff, Leonardo A M; Minicucci, Marcos F

    2015-11-19

    The aim of this study was to evaluate the effects of tomato supplementation on the normal rat heart and the role of oxidative stress in this scenario. Male Wistar rats were assigned to two groups: a control group (C; n = 16), in which animals received a control diet + 0.5 mL of corn oil/kg body weight/day, and a tomato group (T; n = 16), in which animals received a control diet supplemented with tomato +0.5 mL of corn oil/kg body weight/day. After three months, morphological, functional, and biochemical analyses were performed. Animals supplemented with tomato had a smaller left atrium diameter and myocyte cross-sectional area (CSA) compared to the control group (C group: 474 (415-539); T group: 273 (258-297) µm²; p = 0.004). Diastolic function was improved in rats supplemented with tomato. In addition, lipid hydroperoxide was lower (C group: 267 ± 46.7; T group: 219 ± 23.0 nmol/g; p = 0.039) in the myocardium of rats supplemented with tomato. Tomato intake was also associated with up-regulation of miR-107 and miR-486 and down-regulation of miR-350 and miR-872. In conclusion, tomato supplementation induces changes in miRNA expression and reduces oxidative stress. In addition, these alterations may be responsible for CSA reduction and diastolic function improvement.

  4. Burkholderia caballeronis sp. nov., a nitrogen fixing species isolated from tomato (Lycopersicon esculentum) with the ability to effectively nodulate Phaseolus vulgaris.

    Science.gov (United States)

    Martínez-Aguilar, Lourdes; Salazar-Salazar, Corelly; Méndez, Rafael Díaz; Caballero-Mellado, Jesús; Hirsch, Ann M; Vásquez-Murrieta, María Soledad; Estrada-de los Santos, Paulina

    2013-12-01

    During a survey of Burkholderia species with potential use in agrobiotechnology, a group of 12 strains was isolated from the rhizosphere and rhizoplane of tomato plants growing in Mexico (Nepantla, Mexico State). A phylogenetic analysis of 16S rRNA gene sequences showed that the strains are related to Burkholderia kururiensis and Burkholderia mimosarum (97.4 and 97.1 %, respectively). However, they induced effective nitrogen-fixing nodules on roots of Phaseolus vulgaris. Based on polyphasic taxonomy, the group of strains represents a novel species for which the name Burkholderia caballeronis sp. nov. is proposed. The type species is TNe-841(T) (= LMG 26416(T) = CIP 110324(T)).

  5. Potential pollinators of tomato, Lycopersicon esculentum (Solanaceae), in open crops and the effect of a solitary bee in fruit set and quality.

    Science.gov (United States)

    Santos, A O R; Bartelli, B F; Nogueira-Ferreira, F H

    2014-06-01

    We identified native bees that are floral visitors and potential pollinators of tomato in Cerrado areas, described the foraging behavior of these species, and verified the influence of the visitation of a solitary bee on the quantity and quality of fruits. Three areas of tomato crops, located in Minas Gerais, Brazil, were sampled between March and November 2012. We collected 185 bees belonging to 13 species. Exomalopsis (Exomalopsis) analis Spinola, 1853 (Hymenoptera: Apidae) was the most abundant. Ten species performed buzz pollination. Apis mellifera L. 1758 (Hymenoptera: Apidae) and Paratrigona lineata (Lepeletier, 1836) (Hymenoptera: Apidae) could also act as pollinators. The fruit set and number of seeds obtained from the pollination treatment by E. analis were higher than those in the control group. Our results allowed the identification of potential tomato pollinators in Cerrado areas and also contributed information regarding the impact of a single species (E. analis) on fruit set and quality. Although most of the visiting bees show the ability for tomato pollination, there is an absence of adequate management techniques, and its usage is difficult with the aim of increasing the crop production, which is the case for E. analis. Species such as Melipona quinquefasciata, P. lineata, and A. mellifera, which are easy to handle, are not used for pollination services. Finally, it is suggested that a combination of different bee species that are able to pollinate the tomato is necessary to prevent the super-exploitation of only a single species for pollination services and to guarantee the occurrence of potential pollinators in the crop area.

  6. Molecular characterization of plant growth promoting rhizobacteria that enhance peroxidase and phenylalanine ammonia-lyase activities in chile (Capsicum annuum L.) and tomato (Lycopersicon esculentum Mill.).

    Science.gov (United States)

    Sharma, Alok; Pathak, Ashutosh; Sahgal, Manvika; Meyer, Jean-Marie; Wray, Victor; Johri, Bhavdish N

    2007-11-01

    Pythium and Phytophthora species are associated with damping-off diseases in vegetable nurseries and reduce seedling stand and yield. In this study, bacterial isolates were selected on the basis of in vitro antagonism potential to inhibit mycelial growth of damping-off pathogens along with plant growth properties for field assessment in wet and winter seasons. We demonstrate efficacy of bacterial isolates to protect chile and tomato plants under natural vegetable nursery and artificially created pathogen-infested (Pythium and Phytophthora spp.) nursery conditions. After 21 days of sowing, chile and tomato plants were harvested and analysed for peroxidase and phenylalanine ammonia-lyase activities. Pseudomonas sp. strains FQP PB-3, FQA PB-3 and GRP(3 )were most effective in increasing shoot length (P > 0.05%) in both artificial and natural field sites. For example, Pseudomonas sp. FQA PB-3 treatment increased shoot length by 40% in the artificial Pythium 4746 infested nursery site in chile plants in the wet season. The bacterial treatments significantly increased the activity of peroxidase and phenylalanine ammonia-lyase in chile and tomato plant tissues, which are well known as indicators of an active lignification process. Thus, we conclude that treatment with potential bacterial plant growth promoting agents help plants against pathogen invasion by modulating plant peroxidase and phenylalanine ammonia-lyase activities.

  7. Influence of thermal processing on hydrolysis and stability of folate poly-gamma-glutamates in broccoli (Brassica oleracea var. italica), carrot (Daucus carota) and tomato (Lycopersicon esculentum).

    Science.gov (United States)

    Munyaka, Ann Wambui; Verlinde, Philippe; Mukisa, Ivan Muzira; Oey, Indrawati; Van Loey, Ann; Hendrickx, Marc

    2010-04-14

    The folate poly-gamma-glutamate profile, their concentrations, and hydrolysis by endogenous gamma-glutamyl hydrolase (GGH) were evaluated in broccoli, carrot and tomato. Further studies on the effect of time and temperature on folate poly-gamma-glutamate hydrolysis and stability were carried out in broccoli since this vegetable showed the highest long-chain and total folate poly-gamma-glutamate concentration. The evolution of l-ascorbic acid, total phenols and Trolox equivalent antioxidant capacity (TEAC) values was evaluated in parallel. Upon thermal inactivation of GGH prior to crushing, it was observed that broccoli, carrot and tomato contained poly-gamma-glutamates with one to seven glutamate residues but differed in the predominant poly-gamma-glutamates. Crushing of raw broccoli, carrot and tomato resulted in significant poly-gamma-glutamate profile changes in broccoli and carrot (indicating GGH-catalyzed hydrolysis) but not in tomato. In this study, the actual crushing of raw broccoli matrix had a greater effect on folate poly-gamma-glutamate hydrolysis than incubation conditions (0-30 min at 25-55 degrees C). During treatments at 25-140 degrees C, folate retention was higher at 80 and 100 degrees C than at the other temperatures. A similar trend in thermal stability was observed for folates, vitamin C, total phenols and TEAC value, an indication that conditions that result in endogenous antioxidants degradation might also result in folate degradation.

  8. Redox-mediated decolorization of Direct Red 23 and Direct Blue 80 catalyzed by bioaffinity-based immobilized tomato (Lycopersicon esculentum) peroxidase.

    Science.gov (United States)

    Matto, Mahreen; Husain, Qayyum

    2008-10-01

    The aim of this study was to investigate the role of concanavalin A (Con A)-cellulose-bound tomato peroxidase for the decolorization of direct dyes. Cellulose was used as an inexpensive material for the preparation of bioaffinity support. Con A-cellulose-bound tomato peroxidase exhibited higher efficiency in terms of dye decolorization as compared to soluble enzyme under various experimental conditions. Both Direct Red 23 and Direct Blue 80 dyes were recalcitrant to the action of enzyme without a redox mediator. Six compounds were investigated for redox-mediating property. Immobilized peroxidase decolorized both dyes to different extent in the presence of all the used redox mediators. However, 1-hydroxybenzotriazole emerged as a potential redox mediator for tomato peroxidase catalyzed decolorization of direct dyes. These dyes were maximally decolorized at pH 6.0 and 40 degrees C by soluble and immobilized peroxidase. The absorption spectra of the untreated and treated dyes exhibited a marked difference in the absorption at various wavelengths. Immobilized tomato peroxidase showed a lower Michaelis constant than the free enzyme for both dyes. Soluble and immobilized tomato peroxidase exhibited significantly higher affinity for Direct Red 23 compared to Direct Blue 80.

  9. The reorganization of root anatomy and ultrastructure of syncytial cells in tomato (Lycopersicon esculentum Mill. infected with potato cyst nematode (Globodera rostochiensis Woll.

    Directory of Open Access Journals (Sweden)

    Sylwia Fudali

    2011-01-01

    Full Text Available The sequence of anatomical and ultrastructural events leading to the syncytium development in tomato roots infected with Globodera rostochiensis was examined. The syncytia were preferentially induced in cortical or pericyclic cells in the elongation zone of root. They developed towards the vascular cylinder by incorporation of new cells via local cell wall breakdown. After surrounding primary phloem bundle and reaching xylem tracheary elements syncytia spread along vascular cylinder. Roots in primary state of growth seemed to be the best place for syncytium induction as syncytia formed in the zone of secondary growth were less hypertrophied. At the ultrastructural level syncytial elements were characterized by strong hypertrophy, breakdown of central vacuole, increased volume of cytoplasm, proliferation of organelles, and enlargement of nuclei. On the syncytial wall adjoining vessels the cell wall ingrowths were formed, while the syncytial walls at interface of phloem were considerably thickened. They lacked of functional plasmodesmata and did not form any ingrowths. Using immunofluorescent-labelling and immunogold-labelling methods tomato expansin 5 protein was localized in nematode infected roots. The distribution of LeEXP A5 was restricted only to the walls of syncytia. The protein distribution pattern indicated that LeEXP A5 could mediates cell wall expansion during hypertrophy of syncytial elements.

  10. Induction of protection against the necrotrophic pathogens Phytophthora citrophthora and Alternaria solani in Lycopersicon esculentum Mill. by a novel synthetic glycoside combined with amines.

    Science.gov (United States)

    Flors, Víctor; Miralles, M Carmen; González-Bosch, Carmen; Carda, Miguel; García-Agustín, Pilar

    2003-04-01

    1,2,3,4-tetra-O-acetyl-6-ethyladipate-beta- D-glucopyranose (TOGE), a glycoside derivative of adipic acid monoethyl ester and 1,2,3,4-tetra-O-acetyl-beta- D-glucopyranose, was synthesized and the resistance-inducing activity shown by TOGE-1 (TOGE plus furfurylamine) and TOGE-2 (TOGE plus 1,3-diaminepropane) was assayed. TOGE-1 and TOGE-2 protected tomato plants against two different fungal pathogens Phytophthora citrophthora and Alternaria solani. Foliar treatments at very low concentrations (2.5 mg l(-1) TOGE, 0.5 mg l(-1) amine) clearly reduced the disease incidence for both pathogens. TOGE-2 application was the most effective on intact plants as well as on detached leaves, reducing fungal growth by more than 46% with respect to control plants. On the other hand TOGE-1 treatment reduced fungal advance by 21%. These results demonstrate a high protective effect against fungal infections for both chemicals. A possible direct antimicrobial effect was discounted due to the weak activity observed in vitro against these pathogens at the low concentrations used in plants. TOGE-2 treatment clearly activates resistance against both pathogens and improves the protective effect previously shown by FGA mixture (adipic acid monoethyl ester, 1,2,3,4-tetra-O-acetyl-beta- D-glucopyranose and furfurylamine) [V. Flors et al. (2001) J Agric Food Chem 49:2569-2575]. The obtained results indicate that TOGE-1 and TOGE-2 act as resistance inducers. Although their mode of action is still unknown, pre-challenge studies have demonstrated the induction of the phenylpropanoid pathway and antioxidant activities. Both chemicals have demonstrated a beneficial effect on plant development, increasing chlorophyll and protein contents, photosynthetic rate and water-use efficiency. The improvement of plant growth and development produced by these treatments suggests crop tolerance to these chemicals, although effective formulations that are safe to humans must be developed before this technology can be used commercially.

  11. BIOFERTILIZATION USING RHIZOBACTERIA AND AMF IN THE PRODUCTION OF TOMATO (Lycopersicon esculentum Mill. AND ONION (Allium cepa L. SEEDLINGS. II. ROOT COLONIZATION AND NUTRITIONAL STATUS

    Directory of Open Access Journals (Sweden)

    L. E. Pulido

    2003-01-01

    Full Text Available Como complemento a estudios precedentes de la biofertilización en posturas de plantas hortícolas sobre suelos Ferralíticos Rojos compactados, eútricos y en áreas experimentales de la Universidad de Ciego de Ávila, se evaluaron los efectos de la inoculación simple y la coinoculación, mediante el recubrimiento de semillas y sin aplicar fertilizantes minerales, con rizobacterias promotoras del crecimiento vegetal -RPCV- (Azospirillum brasilense, Azotobacter chroococcum y Burkholderia cepacia y hongos micorrízicos arbusculares - HMA- (Glomus clarum y G. fasciculatum en algunos indicadores de la colonización radical por los microorganismos y el estado nutricional de plántulas de tomate y cebolla. A partir de los resultados, se evidenció que, para ambos cultivos, las poblaciones de A. chroococcum, B. cepacia y A. brasilense se incrementaron significativamente en aquellos tratamientos inoculados con estas rizobacterias, encontrando, en general, los mayores valores en los tratamientos que fueron coinoculados. Respecto a la micorrización, los mayores porcentajes de colonización micorrízica y masa del endófito en tomate se obtuvieron mediante la coinoculación de A. brasilense con ambas especies de HMA y, para la cebolla, la máxima colonización la realizó G. fasciculatum aplicada de forma independiente, mientras que la masa del endófito fue mayor en la coinoculación de G. clarum + A. chroococcum. En relación con el estado nutricional de las plantas, en tomate, los tratamientos con presencia conjunta de A. brasilense y ambas especies de HMA fueron los que hicieron mayores extracciones de N y estuvieron entre los que realizaron mayores extracciones de P y K. En cebolla, todos los tratamientos inoculados con ambos tipos de microorganismos fueron capaces de extraer mayores cantidades de N, P y K. Todos estos resultados permiten explicar las causas de la obtención de posturas de adecuada calidad mediante la biofertilización sin el uso de fertilizantes minerales.

  12. 番茄心室形成的遗传规律初探%Studies on the Inheritance of Locule Formation in Tomatoes (Lycopersicon esculentum Mill.)

    Institute of Scientific and Technical Information of China (English)

    李悦; 李天来; 王丹

    2007-01-01

    To fully understand the tomato ovary locule formation of inheritance,two varieties,ovary multi-locule (MLK1) and few-locule (FL1),were used in these studies.Using reciprocal cross,self-cross,and backcross,we have constructed various groups to seek the genetic law and mechanisms of tomato locule formation,for modifying the tomato breeding theory.The parental and the resulting F1 and F2 generations have been used to examine the heredity of the locule number.The results showed that few-locule was incompletely dominant.The data from backcross demonstrated a significant effect on the locule number,whereas,the reciprocal cross showed an insignificant effect.Not surprisingly,the locule number was controlled by nuclear genomes,not by exogenous substances.The model that inherited the locule number belonged to the additive-dominant model: additive effects played a very important role and were partially dominant.In addition,the results revealed that the locule number in tomato was mainly controlled by a single gene,whereas,it was modulated by a number of other genes.Finally,the general inheritability and narrow inheritability of the locule number were 69.44% and 52.98%,respectively.%应用番茄多心室自交系和少心室自交系进行正交、反交及F1代自交和回交,对双亲及获得的正反交F1、F2及回交各世代植株的心室数进行遗传分析.结果表明:少心室为不完全显性,回交效应显著,正反交差异不显著,呈现核遗传,细胞质作用不显著.番茄心室数符合加性-显性模型,以加性效应为主,属不完全显性;控制心室数的最少主基因对数是1对,还受到其他微效基因的控制.番茄心室数广义遗传力为69.44%,狭义遗传力为52.98%.

  13. Comparative evaluation of biochemical changes in tomato (Lycopersicon esculentum Mill. infected by Alternaria alternata and its toxic metabolites (TeA, AOH and AME

    Directory of Open Access Journals (Sweden)

    Mukesh Meena

    2016-09-01

    Full Text Available In the present study, we have evaluated the comparative biochemical defense response generated against Alternaria alternata and its purified toxins viz. alternariol (AOH, alternariol monomethyl ether (AME and tenuazonic acid (TeA. The necrotic lesions developed due to treatment with toxins were almost similar as those produced by pathogen, indicating the crucial role of these toxins in plant pathogenesis. An oxidative burst reaction characterized by the rapid and transient production of a large amount of reactive oxygen species (ROS occurs following the pathogen infection/toxin exposure. The maximum concentration of H2O2 produced was reported in pathogen infected samples (22.2-fold at 24 h post inoculation followed by TeA (18.2-fold, AOH (15.9-fold, and AME (14.1-fold in treated tissues. DAB staining predicted the possible sites of H2O2 accumulation while the extent of cell death were measured by Evans blue dye. The extent of lipid peroxidation and MDA content was higher (15.8-fold at 48 h in the sample of inoculated leaves of pathogen, when compared to control. The cellular damages were observed as increased MDA content and reduced chlorophyll. The activities of antioxidative defense enzymes increased in both pathogen infected as well as toxin treated samples. SOD, activity was 5.9- fold higher at 72 h post inoculation in leaves followed by TeA (5.0-fold, AOH (4.1-fold and AME (2.3-fold treated leaves than control. Catalase activity was found to be increased upto 48 h post inoculation and maximum in pathogen challenged samples followed by other toxins. The native PAGE results showed the variations in the intensities of isozyme (SOD and CAT bands in the pathogen infected and toxin treated samples. APx and GR activities followed the similar trend to scavenge the excess H2O2. The reduction in CAT activities after 48 h post inoculation demonstrate that the biochemical defense programming shown by the host against the pathogen is not well efficient resulting in the compatible host-pathogen interaction. The elicitor (toxins induced biochemical changes depends on the potential toxic effects (extent of ROS accumulation, amount of H2O2 produced. Thus, a fine tuning occurs for the defense related antioxidative enzymes against detoxification of key ROS molecules and effectively regulated in tomato

  14. Effect of different growing substrates on the plant water relations and marketable fruit yield greenhouse-grown tomato (Lycopersicon esculentum Mill.

    Directory of Open Access Journals (Sweden)

    Edward Borowski

    2012-10-01

    Full Text Available In the period 2009-2011, a study was conducted in a greenhouse, using fertigation, to determine water relations and fruit yield of tomato grown in different substrates. Tomato plants were grown on rockwool slabs, 15 dm3  in volume, and on slabs of the same volume made of the following straw chaff: rape straw; rape straw + peat (3:1; rape straw + pine bark (3:1; triticale straw; triticale straw + peat (3:1; triticale straw + pine bark (3:1. 2 tomato plants were grown on each slab, leaving 22 fruit clusters on each plant during the period from February to October. The obtained results showed that water potential, stomatal conductance, transpiration, water saturation deficit, and leaf free proline content in tomato grown on rockwool and on rape or triticale straw chaff substrates did not differ statistically significantly. Also, no significant differences were found in marketable tomato fruit yield and dry matter content in tomato fruits. Peat or pine bark addition to rape or triticale straw substrates had no significant effect on the change in their commercially useful traits. In the opinion of the present authors, substrates made of rape or triticale straw alone, and even more so with the addition of peat or bark, are not inferior in any way to commonly used rockwool.

  15. Perfeccionamiento del sustrato en el modelo V.S.P. para la producción de plántulas de tomate Lycopersicon esculentum Mill

    Directory of Open Access Journals (Sweden)

    Gómez Zambrano Jairo

    1996-12-01

    Full Text Available

    To the sustrate (filter press cake of sugar cane and botton fly ash 3:1 v/v poultry manure and lombricompost from cow dung at levels of 0,6 and 12% v/v were aditioned and soluble sources of Nitrogen (0,150, 300, phosphorus (0,100,200, iron (0,40,60 and copper (0,20,40 g/m3.  It were planted tomato seeds of Santa Clara variety in plastic cups. The experimental desing was one complete randomnized with eigh repetitions. After 35 days the high (cm, the breadth (cm, the number of leaves, the stem diameter and the fresh and dry weight of foliage and root system wre evaluates. The V. S. P. model can be perfected with the combinated aditions of lombricompost in leveIs of 6 and 12% v/v and 150 to 300 g. of Nitrogen as urea applied in solution to the cups before the sowing. It was relevant the mixture of 6% of lombricompost plus 300 9 of Nitrogen. The phosphorus had a minor effect. The Fe and the Cu don't had effect upon the model.

    Al sustrato (3 partes de cachaza y 1 carbonilla v/v se adicionaron gallinaza y lombricompuestos de bovinaza en niveles de 0,6 y 12% v/v y fuentes solubles de Nitrógeno (0,150,300, Fósforo (0,100,200, Hierro (0,40,60 y Cobre (0,20,40 g/rrf3. En vasos plásticos de 330 mi, se sembraron semillas de tomate de la variedad Santa Clara. El diseño experimental fue completamente al azar con 8 repeticiones. A los treinta y cinco días se evaluaron las variables altura de planta (cm, envergadura (cm, peso fresco y seco del follaje y de la raíz y grosor del tallo. El modelo V.S.P. es perfeccionable con las adiciones combinadas de lombricompuesto, en niveles de 6 y 12% v/v y 150 ó 300 g. de nitrógeno como úrea aplicado en forma líquida a los vasos como abonado de fondo, antes de la siembra de las semillas. Fue muy relevante la mezcla de 6% de lombricompuesto con 300 9 de nitrógeno. El fósforo tuvo menor efecto mejorador del modelo. El Cu y el Fe tuvieron menos efecto que el logrado con N y P.

  16. Functional analyses of nanoparticle toxicity: a comparative study of the effects of TiO2 and Ag on tomatoes (Lycopersicon esculentum).

    Science.gov (United States)

    Song, Uhram; Jun, Heeju; Waldman, Bruce; Roh, Jinkyu; Kim, Younghun; Yi, Jongheop; Lee, Eun Ju

    2013-07-01

    Engineered nanoparticles (NPs), increasingly used in industry, enter and migrate through biological ecosystems. NPs may create some acute toxicity, but their overall effects on living organisms remain largely unknown. In particular, the behavior of NPs in natural conditions and their consequent ecological effects are still poorly understood. In this study, we developed methods to test the phytotoxicity of two distinctly different NPs, one aerosol (nano-TiO2), and the other colloidal silver (AgNP), by specifically considering their tendencies to agglomerate and form precipitates. First we examined effects of these NPs on germination and root elongation. While exposure to neither of these NPs resulted in acute toxicity on germination, silver NPs caused significantly decreased root elongation at every concentration we tested. We found that the hydrodynamic diameters of AgNPs were much smaller than those of nano-TiO2, which induced higher uptake and phytotoxicity. Based on the agglomeration behavior of the NPs, greenhouse trials were run using commercial soil, for nano-TiO2, and Hoagland's solution, for AgNP. Phytotoxicity of silver NPs in the mature plants was demonstrated by lower chlorophyll contents, higher superoxide dismutase activity and less fruit productivity, while nano-TiO2 resulted in higher superoxide dismutase activity at the highest concentration (5000mg/kg). Both nano-TiO2 and AgNPs were taken up into plant stems, leaves and fruits. Our results suggest that further studies of the ecological effects of nanoparticles and steps to mitigate appropriate management strategies are required.

  17. USO COMBINADO DE MICROORGANISMOS BENÉFICOS Y PRODUCTOS BIOACTIVOS COMO ALTERNATIVA PARA LA PRODUCCIÓN DE TOMATE (Lycopersicon esculentum, Mill

    Directory of Open Access Journals (Sweden)

    Elein Terry

    2005-01-01

    Full Text Available Con el objetivo de conocer la efectividad agrobiológica de la combinación de microorganismos benéficos y productos bioactivos, sobre el desarrollo, rendimiento y la calidad interna en el cultivo del tomate (variedad 'Amalia', se llevó a cabo un experimento de campo en el 2000 y 2001, sobre un suelo Ferralítico Rojo lixiviado típico. Los tratamientos consistieron en plantas coinoculadas (Glomus clarum- Azospirillum brasilense y asperjadas foliarmente con el producto bioactivo Biostan en diferentes momentos del desarrollo de las plantas. Los resultados mostraron una relación armónica y ecológicamente compatible entre ambos productos, permitiendo un adecuado estado nutricional de las plantas, así como posibilitó un rendimiento de 35 t.ha-1, siendo superior en un 17 % al testigo de producción; por otra parte, los frutos obtenidos con estos tratamientos tuvieron una mejor calidad bromatológica.

  18. Molecular cloning and characterization of ETHYLENE OVERPRODUCER 1-LIKE 1 gene, LeEOL1, from tomato (Lycopersicon esculentum Mill.) fruit.

    Science.gov (United States)

    Zhu, Hong-Liang; Zhu, Ben-Zhong; Shao, Yi; Lin, Xi-Jin; Wang, Xiao-Guang; Gao, Hong-Yan; Xie, Yun-Hong; Li, Ying-Cong; Luo, Yun-Bo

    2007-04-01

    Recently, ETHYLENE OVERPRODUCER 1 (ETO1) had been cloned and identified as a negative post-transcriptional regulator in the ethylene biosynthesis in Arabidopsis. However, little was known about the role of ETO1 in other species, especially in tomato, which was an ideal model for studying the biosynthesis of ethylene during tomato fruit ripening. In this study, a tomato ETHYLENE OVERPRODUCER 1-LIKE 1 (LeEOL1) was cloned. The LeEOL1 cDNA was 3,515 bp long and carried an ORF that putatively encoded a polypeptide of 886 amino acids with a predicted molecular mass of 95 kDa. It shared 74% identity in amino acid sequence with Arabidopsis EOL1 and had one BTB (Broad-complex, Tramtrack, Bric-à-brac) domain and two TPR (tetratricopeptide repeat) domains, which were also conserved domains in AtEOL1. RT-PCR analysis of the temporal expression of LeEOL1 showed that its transcript decreased companied with increase of ethylene production in tomato ripening. The level of LeEOL1 transcripts in wild type tomato fruit at mature green stage did not distinctively change when treated with exogenous ethylene.

  19. Antibacterial and anticandidal activity of Tylosema esculentum (marama) extracts

    OpenAIRE

    Amanda Minnaar; Avrelija Cencic; Martha Kandawa-Schulz; Jose Jackson; Petrina T. Kapewangolo; Sam O. Yeboah; Runner T. Majinda; C.J. Schoeman; Yolandi van Zyl; Gyebi Duodu; Walter Chingwaru

    2011-01-01

    Bean and tuber extracts of Tylosema esculentum (marama) – an African creeping plant – were obtained using ethanol, methanol and water. Based on information that T. esculentum is used traditionally for the treatment of various diseases, the antibacterial and anticandidal effects of tuber and bean extracts were investigated. The antimicrobial activity of the extracts was tested on methicillin-resistant Staphylococcus aureus (MRSA, A...

  20. Improvement of regeneration of Lycopersicon pennellii protoplasts by decreasing ethylene production

    NARCIS (Netherlands)

    Rethmeier, N.O.M.; Jansen, C.E.; Snel, E.A.M.; Nijkamp, H.J.J.; Hille, J.

    1991-01-01

    Lycopersicon pennellii shoots, cultured in vitro for more than a year (type I plants) produced few viable protoplasts in contrast to shoots cultured in vitro for less than five months (type II plants). Ethylene production of both plant types was compared. The low viability of plant type I protoplast

  1. Exploring genetic variation in the tomato (Solanum section Lycopersicon) clade by whole-genome sequencing

    NARCIS (Netherlands)

    Aflitos, S.; Schijlen, E.; de Jong, H.; de Ridder, D.; Smit, S.; Finkers, R.; Wang, J.; Zhang, G.; Li, N.; Mao, L.; Bakker, F.; Dirks, R.; Breit, T.; Gravendeel, B.; Huits, H.; Struss, D.; Swanson-Wagner, R.; van Leeuwen, H.; van Ham, R.C.H.J.; Fito, L.; Guignier, L.; Sevilla, M.; Ellul, P.; Ganko, E.; Kapur, A.; Reclus, E.; de Geus, B.; van de Geest, H.; te Lintel Hekkert, B.; van Haarst, J.; Smits, L.; Koops, A.; Sanchez-Perez, G.; van Heusden, A.W.; Visser, R.; Quan, Z.; Min, J.; Liao, L.; Wang, X.; Wang, G.; Yue, Z.; Yang, X.; Xu, N.; Schranz, E.; Smets, E.; Vos, R.; Rauwerda, J.; Ursem, R.; Schuit, C.; Kerns, M.; van den Berg, J.; Vriezen, W.; Janssen, A.; Datema, E.; Jahrman, T.; Moquet, F.; Bonnet, J.; Peters, S.

    2014-01-01

    We explored genetic variation by sequencing a selection of 84 tomato accessions and related wild species representative of the Lycopersicon, Arcanum, Eriopersicon and Neolycopersicon groups, which has yielded a huge amount of precious data on sequence diversity in the tomato clade. Three new referen

  2. THE EFFICIENCY OF NEW INSECTICIDE CARBAMATE IN CHEMICAL CONTROL OF TOMATO LEAFMINER (Scrobipalpuloides absoluta MEYRIC, 1917 IN TOMATO PLANTS (Lycopersicon esculentum, MILL EFICIÊNCIA DE NOVO INSETICIDA CARBAMATO NO CONTROLE QUÍMICO DA TRAÇA (Scrobipalpuloides absoluta Meyric1917 SOBRE TOMATEIRO (Lycopersicon esculentum, Mill

    Directory of Open Access Journals (Sweden)

    Antônio Lopes da Silva

    2007-09-01

    Full Text Available

    The tomato leafminer (Scrobipalpuloides absoluta, a common pest of tomato plants, is known in many regions in Brazil. In order to determine the efficiency of new insecticide alanycarb, to control the tomato leafminer a field experiment was carried out in Goiânia, Goiás, Brazil. The treatments and dosage of the insecticides per hectare were: alanycarb 300: 1.0, 1.5 and 2.0 l; cartap 500 BR: 2.0 kg; abamectin 18 CE: 1.0 1 plus an untreated check. The results obtained in this experiment showed that all insecticides were efficient in controlling the tomato leafminer until 8 days after treatment application and they were able to increase productivity.

    KEY-WORDS: Scrobipalpuloides absoluta; tomato leafminer; chemical control.

    O experimento foi realizado na área de horticultura da Escola de Agronomia da Universidade Federal de Goiás, visando ao controle químico da traça do tomateiro (Scrobipalpuloides absoluta Meyric, 1917, no período de setembro a dezembro de 1995, para avaliar a eficiência do inseticida Onic 300 (Alanycarb nas dosagens de 1,0, 1,5 e 2,0 litros do produto formulado por ha, comparando-o com Cartap 500 BR (Cartap na dosagem de 2,4 kg/ha, Vertimec 18 CE (Abamectin na dosagem de 1,0 l/ha misturado a um óleo mineral e uma testemunha. Pelos resultados obtidos concluiu-se que todos os inseticidas utilizados controlaram eficientemente a traça em tomateiro até 8 dias após aplicação e que todos os tratamentos promoveram aumento de produtividade.

    PALAVRAS-CHAVE: Traça; Scrobipalpuloides absoluta; controle químico; tomateiro.

  3. Bioatividade de produtos fitossanitários utilizados na cultura do tomateiro (Lycopersicon esculentum Mill. a Trichogramma pretiosum Riley, 1879 (Hymenoptera: Trichogrammatidae nas gerações F1 e F2 Bioactivity of pesticides used in tomato (Lycopersicon esculentum Mill. crop to Trichogramma pretiosum Riley, 1879 (Hymenoptera: Trichogrammatidae in F1 and F2 generations

    Directory of Open Access Journals (Sweden)

    Geraldo Andrade Carvalho

    2003-04-01

    Full Text Available A bioatividade de dezoito produtos químicos utilizados no controle de pragas e doenças do tomateiro, sobre duas linhagens de Trichogramma pretiosum Riley, 1879 (L9=Alegre, ES e L10= Venda Nova do Imigrante, ES, nas gerações F1 e F2, foi investigada em laboratório. Ovos de Anagasta kuehniella (Zeller contendo o parasitóide em diferentes estágios de desenvolvimento (ovo-larva, pré-pupa e pupa foram tratados por meio de imersão nas respectivas caldas químicas. Os inseticidas triflumuron, clorfluazuron, deltametrina, Bacillus thuringiensis, lambdacialotrina, teflubenzuron, acefato, pirimicarb e ciromazina, e os fungicidas benomil, iprodiona, clorotalonil e dimetomorf, independente da linhagem, não reduziram a longevidade das fêmeas de T. pretiosum da geração F1. Os inseticidas abamectin, cartap, metamidofós e lambdacialotrina afetaram a razão sexual de indivíduos da geração F1, e não reduziram a taxa de emergência de parasitóides da F2, independente do estágio de desenvolvimento e da origem da população de T. pretiosum. Parasitóides de Venda Nova do Imigrante, ES (L10 mostraram-se mais susceptíveis que os de Alegre, ES (L9 aos efeitos dos compostos avaliados. De modo geral, a fase de pupa de T. pretiosum, independente da população, apresentou maior tolerância aos produtos testados. Recomenda-se a realização de novos testes para outras populações desse parasitóide que serão utilizadas no controle de pragas, pois podem responder de forma diversa aos produtos fitossanitários avaliados.The bioactivity of eighteen pesticides commonly used to pests and diseases control on tomato, in two strains of Trichogramma pretiosum Riley, 1879 (L9=Alegre, ES and L10= Venda Nova do Imigrante, ES, Brazil in F1 and F2 generations, was evaluated under laboratory conditions. The bioassays were conducted in a climatic chamber at 25 ± 2 ºC, 60 ± 10% RH and 14 hours of photophase. Eggs of Anagasta kuehniella (Zeller containing the parasitoid in different phases of development (egg-larva, pre-pupa and pupa were treated by immersion in aqueous dilutions of the products. The insecticides triflumuron, chlorfluazuron, deltamethrin, Bacillus thuringiensis, lambdacyalothrin, abamectin, cartap and methamidophos, did not decrease the longevity of females T. pretiosum in F1 generation independently of the parasitoid strain. Abamectin, cartap, methamidophos and lambdacyalothrin affected the sexual ratio in F1, and did not decrease the emergence of parasitoids in F2 generation, independently of stage of development and origin. The L10 strain was more susceptible to all pesticides as compared to L9. The pupal stage of T. pretiosum was more tolerant to the products, independently of its origin. It is recommended new tests with other strains of this parasitoid which may respond differently to the products used, before field recomendations are made.

  4. Pessegueiro: cultivar BRS kampai Peach cultivar BRS kampai

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    Maria do Carmo Bassols Raseira

    2010-12-01

    Full Text Available RESUMO O programa de Melhoramento Genético de Pessegueiros da Embrapa Clima Temperado tem, entre seus objetivos, a obtenção de cultivares produtoras de frutas para consumo in natura, com características que satisfaçam às exigências dos consumidores. Alguns dos grandes centros consumidores, como é o caso de São Paulo e Curitiba, preferem pêssegos de polpa branca e sabor doce. A cultivar BRS Kampai, obtida de um cruzamento entre 'Chimarrita' e 'Flordaprince', alia a baixa necessidade em frio, o que é uma vantagem em regiões subtropicais, à boa aparência, com sabor superior a qualquer um dos parentais. A colheita dos frutos desta cultivar inicia-se geralmente, em meados de novembro, em Pelotas-RS (em Atibaia, São Paulo, inicia-se na segunda quinzena de outubro, poucos dias antes das cultivares Rubimel (polpa amarela e Premier (polpa branca, sendo ótima substituta para esta última.The peach breeding program of Embrapa Clima Temperado has, among the objectives the development of fresh market cultivars that fulfill the consumer's preference. Some of the largest consumer centers, such as São Paulo and Curitiba, prefer white flesh peaches with sweet flavor. Cultivar BRS Kampai originated from a cross between 'Chimarrita' and 'Flordaprince', adds the low chilling requirement, advantageous for subtropical areas, to the good appearance and flavor superior to any of the parents. The harvest period of this cultivar begins mid November, in Pelotas, RS, a few days before Rubimel (yellow flesh and Premier (white flesh cultivars (and in the second half of October, in Atibaia, SP being a good substitute for the later.

  5. Cultivar development of allogamous crops

    National Research Council Canada - National Science Library

    Souza Jr., Cláudio Lopes de

    2011-01-01

    .... It was reviewed the genetic structure of these species and its relationship with the development of cultivars, the technologies used to develop hybrids and improved open-pollinated varieties and their F1...

  6. How to maintain improved cultivars

    NARCIS (Netherlands)

    Parlevliet, J.E.

    2007-01-01

    Improved cultivars loose their identity and healthiness unless maintained properly. Contaminating and degrading forces, such as outcrossing, volunteer plants, mixing, natural selection, mutation and seed-borne diseases, are at the root of this. Maintenance selection can prevent this deterioration.

  7. BRS Progresso – Rye cultivar

    Directory of Open Access Journals (Sweden)

    Alfredo do Nascimento Junior

    2014-10-01

    Full Text Available The rye cultivar BRS Progresso, developed by the Brazilian Agricultural Research Corporation (Embrapa, is the result of a synthetic cross of 18 open-pollinated, self-incompatible lines, resistant to stem rust.

  8. Micropropagation of four blueberry cultivars

    Directory of Open Access Journals (Sweden)

    Arturo Brenes Angulo

    2015-06-01

    Full Text Available Sharpblue, Woodard, Bonita and Delite cultivars were propagated from leaf explants. Leaf sections from in vitro singlenode explants were compared with those from plants in the field. The explants were placed in WPM medium supplemented with 1.5 mg.l-1 TDZ to promote sprouting, then subcultured to fresh WPM medium supplemented with 0.5 or 1.0 mg.l-1 zeatin to enhance adventitious shoot growth. Shoot clump length and number of shoots >2mm and >1cm in each clump were determined. Shoots 8 to 10 cm were harvested at 8 weeks and subcultured in WPM medium supplemented with 0, 1, 2, 4 or 8 mg.l-1 IBA for rooting. After 10 weeks, shoots were transplanted to greenhouse for acclimatization. The leaf segments cultured in WPM medium with TDZ formed adventitious shoot clumps in all varieties, regardless of leaf source. A significant effect of cultivar, source of explant and interaction cultivar x leaf source was found for shoots >2 mm. Clump size varied significantly among cultivars, leaf source, and interaction cultivars x source x zeatin concentration, when transfering to a medium with 2 concentrations of the growth regulator, but no differences due to cytokinin concentration were found. Number of shoots >2mm and length of the longest shoot varied significantly with cultivar and the interaction cultivar x source of explant in WPM + zeatin medium. Woodard explants from in vitro leaf sections produced the highest number of shoots (44.70±13.34 and Delite the lowest (21.50±4.99. Shoots subcultured in WPM with different IBA concentrations had les than 10% rooting in all treatments. However, when transplanted to greenhouse 100% rooting was obtained, regardless of in vitro treatment with IBA or leaf explant source. All the microshoots of the 4 cultivars were successfully greenhouse acclimatized and plantlets later established in the field.

  9. Occurrence of Candida orthopsilosis in Brazilian tomato fruits (Lycopersicum esculentum Mill.

    Directory of Open Access Journals (Sweden)

    D. Robl

    2014-01-01

    Full Text Available We aimed to isolate and identify yeasts found in the tomato fruit in order to obtain isolates with biotechnological potential, such as in control of fungal diseases that damage postharvest fruits. We identified Candida orthopsilosis strains LT18 and LT24. This is the first report of this yeast on Lycopersicum esculentum fruits in Brazil.

  10. Occurrence of Candida orthopsilosis in Brazilian tomato fruits (Lycopersicum esculentum Mill.)

    OpenAIRE

    Robl, D.; Thimoteo, S.S.; de Souza, G.C.C.F.; M.R. Beux; Dalzoto,P.R.; Pinheiro, R L; PIMENTEL, I. C.

    2014-01-01

    We aimed to isolate and identify yeasts found in the tomato fruit in order to obtain isolates with biotechnological potential, such as in control of fungal diseases that damage postharvest fruits. We identified Candida orthopsilosis strains LT18 and LT24. This is the first report of this yeast on Lycopersicum esculentum fruits in Brazil.

  11. Occurrence of Candida orthopsilosis in Brazilian tomato fruits (Lycopersicum esculentum Mill.).

    Science.gov (United States)

    Robl, D; Thimoteo, S S; de Souza, G C C F; Beux, M R; Dalzoto, P R; Pinheiro, R L; Pimentel, I C

    2014-01-01

    We aimed to isolate and identify yeasts found in the tomato fruit in order to obtain isolates with biotechnological potential, such as in control of fungal diseases that damage postharvest fruits. We identified Candida orthopsilosis strains LT18 and LT24. This is the first report of this yeast on Lycopersicum esculentum fruits in Brazil.

  12. Rheological and pasting properties of buckwheat (Fagopyrum esculentum Moench) flours with and without jet-cooking

    Science.gov (United States)

    Pasting, rheological and water-holding properties of buckwheat (Fagopyrum esculentum) flour obtained from whole achenes separated into three particle sizes, and three commercial flours (Fancy, Supreme and Farinetta) were measured with or without jet-cooking. Fancy had instantaneous paste viscosity ...

  13. Differential expression of flavonoid biosynthesis genes and accumulation of phenolic compounds in common buckwheat (Fagopyrum esculentum).

    Science.gov (United States)

    Li, Xiaohua; Park, Nam Il; Xu, Hui; Woo, Sun-Hee; Park, Cheol Ho; Park, Sang Un

    2010-12-01

    Common buckwheat (Fagopyrum esculentum) is a short-season grain crop that is a source of rutin and other phenolic compounds. In this study, we isolated the cDNAs of 11 F. esculentum enzymes in the flavonoid biosynthesis pathway, namely, phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate:CoA ligase (4CL) 1 and 2, chalcone synthase (CHS), chalcone isomerase (CHI), flavone 3-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), flavonol synthase (FLS) 1 and 2, and anthocyanidin synthase (ANS). Quantitative real-time polymerase chain reaction analysis showed that these genes were most highly expressed in the stems and roots. However, high performance liquid chromatography analysis indicated that their flavonoid products, such as rutin and catechin, accumulated in the flowers and leaves. These results suggested that flavonoids may be transported within F. esculentum. In addition, light and dark growth conditions affected the expression levels of the biosynthesis genes and accumulation of phenolic compounds in F. esculentum sprouts.

  14. Determination of L-ascorbic acid in Lycopersicon fruits by capillary zone electrophoresis.

    Science.gov (United States)

    Galiana-Balaguer, L; Roselló, S; Herrero-Martínez, J M; Maquieira, A; Nuez, F

    2001-09-15

    This study shows an improved method for the determination of L-ascorbic acid (l-AA) in fruits of Lycopersicon by capillary zone electrophoresis (CZE). Two backgrounds electrolytes (BGEs) have been tested: (i) 400 mM borate at pH 8.0 and 1 x 10(-2)% hexadimethrine bromide, for the separation of Eulycopersicon subgenus species; and (ii) as in BGE(i) but supplemented with 20% (v/v) acetonitrile, for the separation of species of the Eriopersicon subgenus. The present procedures were compared with two routine methods-enzymatic assay and potentiometric titration with 2,6-dichlorophenol-indophenol. While these routine methods presented some difficulties in quantifying l-AA in several Lycopersicon fruits, CZE was successfully applied in all the analyzed samples. The proposed CZE protocols give lower detection limits (<0.4 microg ml(-1)); are cheaper, quicker, and highly reproducible; and can be applied to analyze large series of samples (ca. 50 samples per day) which is utmost importance, not only in screening trials for internal quality and tomato breeding programs, but also in systematic and routine characterization of Lycopersicon fruits.

  15. EVALUACIÓN DE LA TOLERANCIA AL CALOR A NIVEL DE PLANTA EN EL GERMOPLASMA DE TOMATE (Solanum L. sección Lycopersicon subsección Lycopersicon) CONSERVADO Ex Situ

    OpenAIRE

    Marilyn Florido; Marta Álvarez; Regla M. Lara; Dagmara Plana; Varela, M.; T. Shagarodsky; Moya, C.

    2009-01-01

    Este trabajo se desarrolló con el objetivo de evaluar la tolerancia al calor en una muestra representativa del germoplasma de tomate (Solanum L. sección Lycopersicon subsección Lycopersicon) conservado ex situ en las colecciones cubanas e identificar las accesiones más tolerantes para su empleo en los programas de mejoramiento genético. Para ello se evaluó el porcentaje de fructificación en los períodos óptimo y estresante (primavera-verano) del cultivo, para calcular los criterios de estrés ...

  16. RELACION ENTRE LA TERMOESTABILIDAD DE LA MEMBRANA, LA VIABILIDAD CELULAR Y LOS CRITERIOS DE TOLERANCIA EN LA EVALUACIÓN DE LA TOLERANCIA AL CALOR EN TOMATE (Solanum L. sección Lycopersicon subsección Lycopersicon)

    OpenAIRE

    Marilyn Florido Bacallao; Marta Álvarez Gil; Mario Varela Nualles; Regla M. Lara Rodríguez; Dagmara Plana Ramos; Tomás Shagarodsky Scull; Carlos Moya López

    2011-01-01

    El presente trabajo se desarrolló con el objetivo deconocer si existe variabilidad en cuanto a tolerancia al calor enuna muestra representativa del germoplasma de tomate (Solanum L. sección Lycopersicon subsección Lycopersicon) conservadoex situ en las colecciones cubanas e identificar las accesionesmás tolerantes para su empleo en los programas de mejoramiento genético. Para ello se calcularon los parámetros estadísticos:media, rango y coeficiente de variación de los caracterestermoestabilid...

  17. IPR 118 - Bread wheat cultivar

    Directory of Open Access Journals (Sweden)

    Carlos Roberto Riede

    2007-01-01

    Full Text Available Wheat cultivar IPR 118 developed by IAPAR has a good yield potential and is widely adapted. It is earlymaturing and moderately tolerant to shattering and soil aluminum, moderately resistant to leaf rust and presents high glutenstrength for bread-making. The overall yield exceeded controls by 13%.

  18. Tylosema esculentum (Marama) Tuber and Bean Extracts Are Strong Antiviral Agents against Rotavirus Infection

    OpenAIRE

    Walter Chingwaru; Runner T. Majinda; Sam O. Yeboah; Jackson, Jose C.; Petrina T. Kapewangolo; Martha Kandawa-Schulz; Avrelija Cencic

    2011-01-01

    Tylosema esculentum (marama) beans and tubers are used as food, and traditional medicine against diarrhoea in Southern Africa. Rotaviruses (RVs) are a major cause of diarrhoea among infants, young children, immunocompromised people, and domesticated animals. Our work is first to determine anti-RV activity of marama bean and tuber ethanol and water extracts; in this case on intestinal enterocyte cells of human infant (H4), adult pig (CLAB) and adult bovine (CIEB) origin. Marama cotyledon ethan...

  19. Stem morphology and vascular anatomy of Fagopyrum esculentum Moench. under retardant chlormequat chloride action

    Directory of Open Access Journals (Sweden)

    Oleksandr E. Smirnov

    2015-05-01

    Full Text Available Effects of different concentrations of retardant chlormequat chloride on hypocotyl and first internode lengths of common buckwheat (Fagopyrum esculentum Moench. were investigated. It was established that treatment of buckwheat plants with chlormequat chloride before presowing led to: reduction of stem length, activation of early and intensive lignification of vascular and mechanical elements, increasing number and size of vascular bundles in correlation with functional reconstructions on donor-acceptor connections of plant organs.

  20. Genetic base of Brazilian irrigated rice cultivars

    Directory of Open Access Journals (Sweden)

    Hudson de Oliveira Rabelo

    2015-08-01

    Full Text Available The aim of this study was to estimate the genetic base of Brazilian irrigated rice cultivars released in the period from 1965 to 2012. The genealogies of the cultivars were obtained based on information from marketing folders, websites, crossings records, and scientific articles. The following factors were calculated: relative genetic contribution (RGC, accumulated genetic contribution (AGC, frequency (in percentage of each ancestor in the genealogy (FAG, number of ancestors that constitute each cultivar (NAC,number of ancestors responsible for 60%, 70%, 80% and 90% of the genetic base (NAGB, and average number of ancestor per cultivar (ANAC. The cultivars were also grouped based on the period of release (1965-1980, 1981-1990, 1991-2000 and 2001-2012. For each grouping, the previously described factors were also estimated. A total of 110 cultivars were studied and it was concluded that the genetic base of Brazilian irrigated rice cultivars is narrow.

  1. 'Gloriosa': cultivar de alface americana tropicalizada 'Gloriosa': crisphead tropicalized cultivar

    Directory of Open Access Journals (Sweden)

    Fernando C. Sala

    2008-09-01

    Full Text Available 'Gloriosa' é uma cultivar de alface (Lactuca sativa L. americana tropicalizada e resistente a Thielaviopsis basicola. Foi selecionada a partir de variantes da cultivar Lucy Brown, pelo método genealógico. Progênies S1 a S6 foram selecionadas para resistência a T. basicola, características hortícolas, tolerância ao pendoamento precoce e adaptação ao cultivo de verão. Apresenta ciclo precoce de 40 a 50 dias pós-transplante, pendoamento lento, cabeças grandes e três camadas de folhas externas. Possui tolerância a Xanthomonas campestris pv. vitians e suporta o cultivo nos períodos de elevadas temperaturas e pluviosidade. Trata-se da primeira cultivar de alface americana tropicalizada no Brasil.'Gloriosa' is a tropicalized crisphead lettuce (Lactuca sativa L. resistant to Thielaviopsis basicola. It was derived by selection from cv. Lucy Brown variants by pedigree method. Progenies S1 to S6 were selected for T. basicola resistance, desirable horticulture traits, slowbolting and adaptation for Brazilian summer crop. It presents a precocious cycle within 40 to 50 days of being planted, slowbolting, large head and three layers of frame levels. It is tolerant to Xanthomonas campestris pv. vitians and is able to be grown under high temperature and rainy summer in southern of Brazil. It is the first crisphead lettuce type developed for tropical regions of Brazil.

  2. Cultivar de soja BRS 205

    Directory of Open Access Journals (Sweden)

    Bonato Emídio Rizzo

    2002-01-01

    Full Text Available A BRS 205, cultivar de soja de ciclo semiprecoce, obtida do cruzamento [BR-16(2 x Ocepar 8] x Tracy-M, é indicada para o Rio Grande do Sul, em semeaduras de novembro. Possui crescimento determinado, plantas de flor branca, pubescência marrom, porte baixo e grãos de hilo preto. Tem resistência ao cancro-da-haste (Diaporthe phaseolorum f. sp. meridionalis, à podridão-parda-da-haste (Phialophora gregata, à mancha-olho-de-rã (Cercospora sojina e à pústula bacteriana (Xanthomonas axonopodis pv. glycines. É moderadamente resistente ao oídio (Microsphaera diffusa. O rendimento médio de grãos foi 6% e 11% superior ao das cultivares RS 7-Jacuí e BR-16, respectivamente.

  3. Microsporogenesis of Apricot Cultivars in Hungary

    Directory of Open Access Journals (Sweden)

    Veronika HAJNAL

    2013-12-01

    Full Text Available Like all phenological processes, microsporogenesis is mainly determined genetically, but its phenotypical expression is greatly influenced by environmental factors, particularly the temperature. During the 3-year experimental period, the process of microsporogenesis was examined in eight apricot cultivars originated in North America and in Romania. The Hungarian cultivar ‘Gönci magyar kajszi’ was used as the control. Based on the results it was possible to rank the cultivars in terms of their microsporogenesis schedule. The same order was found in all three years. This order also indicates the yield reliability of the cultivars. Endodormancy ended between January 10th and 25th in the buds of the earliest cultivar ‘Pinkcot’, but not until February 5-10th in the latest cultivar ‘Harlayne’. Three of the cultivars had faster flower bud development than the control, in the order ‘Pinkcot’, ‘Orange Red’ and ‘Harcot’. Growing these cultivars thus involves greater risk than for ‘Gönci magyar kajszi’ in Hungary, due to their rapid winter flower bud development. Some cultivars found to have slower flower bud development than ‘Gönci magyar kajszi’; ‘Litoral’, ‘Harogem’, ‘Comandor’, ‘Sirena’ and ‘Harlayne’ – thus these cultivars can be grown more reliably.

  4. Ozone sensitivity of currant tomato (Lycopersicon pimpinellifolium), a potential bioindicator species

    Energy Technology Data Exchange (ETDEWEB)

    Iriti, Marcello [Istituto di Patologia Vegetale, Universita di Milano, Via Celoria 2, 20133 Milan (Italy); Belli, Lucia [Istituto di Patologia Vegetale, Universita di Milano, Via Celoria 2, 20133 Milan (Italy); Nali, Cristina [Dipartimento di Coltivazione e Difesa delle Specie Legnose ' G. Scaramuzzi' , Universita di Pisa, Via del Borghetto 80, Pisa (Italy); Lorenzini, Giacomo [Dipartimento di Coltivazione e Difesa delle Specie Legnose ' G. Scaramuzzi' , Universita di Pisa, Via del Borghetto 80, Pisa (Italy); Gerosa, Giacomo [Dipartimento di Matematica e Fisica, Universita Cattolica del Sacro Cuore, Via Musei 41, 25121 Brescia (Italy); Faoro, Franco [CNR, Istituto di Virologia Vegetale, Sezione di Milano, Via Celoria, 2 20133 Milan (Italy)]. E-mail: f.faoro@ivv.cnr.it

    2006-05-15

    The wild tomato species Lycopersicon pimpinellifolium (currant tomato) was exposed to different O{sub 3} concentration, both in controlled environment fumigation facilities and in open-top chambers, to assess its sensitivity and to verify its potential as a bioindicator plant. Plants appeared particularly sensitive to O{sub 3} at an early stage of growth, responding with typical chlorotic spots within 24 h after exposure to a single pulse of 50 ppb for 3 h, and differentiating peculiar symptoms, such as reddish necrotic stipples, bronzing and extensive necrosis, depending on O{sub 3} concentration. Histo-cytochemical investigations with 3,3'-diaminobenzidine, to localize H{sub 2}O{sub 2}, and Evans blue, to detect dead cells, suggested that currant tomato sensitivity to O{sub 3} could be due to a deficiency in the anti-oxidant pools. The combination of these stainings proved to be useful, either to predict visible symptoms, early before their appearance, and to validate leaf ozone injury. - Currant tomato (Lycopersicon pimpinellifolium) was found to be very sensitive to ozone.

  5. Phenotypic response of Lycopersicon chilense to water deficit Respuesta fenotípica de Lycopersicon chilense al deficit hídrico

    Directory of Open Access Journals (Sweden)

    CARLOS MALDONADO

    2003-03-01

    Full Text Available ABSTRACT Environmental-induced phenotypic variation in plants is often considered to be a functional response that maximizes fitness in heterogeneous environments. Lycopersicon chilense, a tomato species endemic to Atacama Desert, shows altitudinal phenotypics variations in their natural environments, which could be due to different soil water availabilities. It is hypothesized that (a seeds coming from populations of different environments, cultivated in the same environment, will have similar phenotypes, if populations are not genetically differentiated, and that (b the different populations subjected to two drought levels should vary their phenotypic constitution with respect to the control groups. The responses of twenty phenotypic traits to different irrigation levels were studied in nine wild populations of Lycopersicon chilense. Seeds were collected from populations along an altitudinal gradient (from 20 m to 3,075 m, transferred to a common environment and grown under three soil water conditions: low (80 % FC, moderate (40 % FC and severe (20 % FC. In spite of the climatic differences in their natural habitat the phenotypic responses of plants growing in the same environment was similar in the nine populations. Significant differences among populations were only observed in three out of twenty traits (fruit fresh weight, fruit volume and number of seeds per fruit. Soil water deficit induced a phenotypic response in twelve characters; among these: root dry weight, cover, number of seeting fruits and number of seeds per fruit showed the highest significance. An interaction between population and drought treatment was found only for fresh weight of fruits, fruit volume and number of seeds per fruit. Our data indicate that the phenotypic response does not differ among populations growing under similar environmental conditions. Probably the phenotypic response of L. chilense in their natural habitats is related to physiological and metabolic

  6. Mosquitocidal properties of Oxystelma esculentum (Asclepiadaceae-Indian medicinal plant tested against Ades aegypti (Linn. (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    Kuppusamy Elumalai

    2015-04-01

    Full Text Available Objective: To evaluate the mosquitocidal activities of various solvent extract of Oxystelma esculentum (O. esculentum against the medically important dengue vector mosquito, Aedes aegypti (Ae. aegypti L. Methods: A total of 25 early third instar larvae of Ae. aegypti were exposed to various concentrations (60-300 mg/L and were assayed in the laboratory by using the protocol of World Health Organization, 2005; the 24 h LC50 values of the O. esculentum leaf extract was determined by probit analysis. The ovicidal activity was determined against the freshly laid eggs of Ae. aegypti to various concentrations ranging from 50-300 mg/L under laboratory conditions. The pupicidal activity was determined against pupae of Ae. aegypti to various concentrations ranging from 70-280 mg/L after 24 h of exposure to the concern extract. The repellent efficacy was determined against adult female mosquito species at 1.0, 2.0 and 3.0 mg/ cm2 under laboratory conditions. Results: The LC50 value of methanol extract of O. esculentum against 3rd instar larvae of Ae. aegypti was 125.82 mg/L. The same extract showed 100% egg mortality at 250 mg/L and also pupicidal activity observed against the pupae of Ae. aegypti at 280 mg/L. Conclusions: The present results suggest that the O. esculentum leaf extracts provided an excellent, potential phytopesticide for controlling Ae. aegypti mosquito.

  7. Mosquitocidal properties of Oxystelma esculentum (Asclepiadaceae)-Indian medicinal plant tested against Ades aegypti (Linn.) (Diptera:Culicidae)

    Institute of Scientific and Technical Information of China (English)

    Kuppusamy Elumalai; Kaliyamoorthy Krishnappa

    2015-01-01

    Objective: To evaluate the mosquitocidal activities of various solvent extract of Oxystelma esculentum (O. esculentum) against the medically important dengue vector mosquito, Aedes aegypti (Ae. aegypti) L. Methods: A total of 25 early third instar larvae of Ae. aegypti were exposed to various concentrations (60-300 mg/L) and were assayed in the laboratory by using the protocol of World Health Organization, 2005; the 24 h LC50 values of the O. esculentum leaf extract was determined by probit analysis. The ovicidal activity was determined against the freshly laid eggs of Ae. aegypti to various concentrations ranging from 50-300 mg/L under laboratory conditions. The pupicidal activity was determined against pupae of Ae. aegypti to various concentrations ranging from 70-280 mg/L after 24 h of exposure to the concern extract. The repellent efficacy was determined against adult female mosquito species at 1.0, 2.0 and 3.0 mg/cm2 under laboratory conditions. Results: The LC50 value of methanol extract of O. esculentum against 3rd instar larvae of Ae. aegypti was 125.82 mg/L. The same extract showed 100% egg mortality at 250 mg/L and also pupicidal activity observed against the pupae of Ae. aegypti at 280 mg/L. Conclusions: The present results suggest that the O. esculentum leaf extracts provided an excellent, potential phytopesticide for controlling Ae. aegypti mosquito.

  8. Fontes de resistência em acessos de Solanum (secção Lycopersicon a Verticillium dahliae raças 1 e 2 Sources of resistance to Verticillium dahliae races 1 and 2 in accessions of Solanum (section Lycopersicon

    Directory of Open Access Journals (Sweden)

    Bruno Eduardo C de Miranda

    2010-12-01

    Full Text Available A murcha-de-verticílio (Verticillium dahliae é uma das doenças mais severas do tomateiro (Solanum lycopersicum L.. Duas raças fisiológicas de V. dahliae foram caracterizadas infectando o tomateiro e ambas estão presentes no Brasil. A estratégia de controle mais efetiva para isolados da raça 1 tem sido a utilização de cultivares resistentes em associação com práticas culturais. Entretanto, não existem cultivares comerciais de tomate resistentes à raça 2 do patógeno. Neste contexto, torna-se importante a identificação de novas fontes de resistência que sejam efetivas contra as duas raças de V. dahliae. Buscou-se fontes de resistência a isolados de V. dahliae (raças 1 e 2. Cem acessos de uma coleção de germoplasma contendo espécies cultivadas e selvagens de Solanum (secção Lycopersicon foram inicialmente inoculados pelo método de imersão de raízes com um isolado de V. dahliae raça 1 (5 mL de uma suspensão de 10(6 conídios/mL em condições de casa de vegetação. A avaliação foi feita aos 30 dias após a inoculação, usando uma escala de notas variando de 1 (planta sadia a 5 (planta morta. Foram considerados resistentes os acessos que apresentaram nota média entre 1 e 2. Um grupo composto por 38 acessos classificados como resistentes neste primeiro ensaio foi reavaliado para resistência a outros quatro isolados de V. dahliae pertencentes às raças 1 e 2. As cultivares Ponderosa e Floradade foram utilizadas como controles. O ensaio foi conduzido em casa de vegetação em delineamento experimental de blocos ao acaso em arranjo fatorial 5 x 40, com três repetições (vasos com quatro plantas. A avaliação foi feita baseada nos parâmetros epidemiológicos: período de incubação e índice de doença. Foram identificados acessos com resistência raça-específica e também com resistência a ambas as raças. Estes acessos podem ser indicados para futuros programas de melhoramento genético visando incorporar

  9. Impact of suboptimal temperature on growth, photosynthesis, leaf pigments and carbohydrates of domestic and high-altitude wild Lycopersicon species

    NARCIS (Netherlands)

    Venema, J.H.; Posthumus, F.S; van Hasselt, P.R

    1999-01-01

    The impact of near-optimal (25/20 degrees C) and suboptimal (16/14 degrees C) day/night temperatures on growth, photosynthesis, pigment composition and carbohydrate content was compared between domestic and high-altitude wild Lycopersicon species. When related to the relative shoot growth rate

  10. Impact of suboptimal temperature on growth, photosynthesis, leaf pigments and carbohydrates of domestic and high-altitude wild Lycopersicon species

    NARCIS (Netherlands)

    Venema, J.H.; Posthumus, F.S; van Hasselt, P.R

    1999-01-01

    The impact of near-optimal (25/20 degrees C) and suboptimal (16/14 degrees C) day/night temperatures on growth, photosynthesis, pigment composition and carbohydrate content was compared between domestic and high-altitude wild Lycopersicon species. When related to the relative shoot growth rate (RSGR

  11. Dicty_cDB: CFJ532 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 2039 |AW622039.1 EST312837 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cD...1828 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX10C9 5', ... 3 AI775579 |AI775579.1 EST256679 tomato resistant, Cornell Lycopersicon esculent

  12. Dicty_cDB: VFD860 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available F051619 |BF051619.1 EST436849 tomato developing/immature green fruit Lycopersicon...9099.1 EST414391 tomato developing/immature green fruit Lycopersicon esculentum c... Cornell University Lycopersicon esculentum cDNA clone cTOC1D13 5', mRNA sequence. 50 0.038 1 BE459099 |BE45

  13. NS Pudarka: A new winter wheat cultivar

    Directory of Open Access Journals (Sweden)

    Hristov Nikola

    2014-01-01

    Full Text Available The high-yielding, medium late winter wheat cultivar NS Pudarka was developed by crossing genetic divergent parents: line NMNH-07 and cv. NS 40S and Simonida. In cultivar NS Pudarka genes responsible for high yield potential, very good technological quality, resistance to lodging, low temperature and diseases, were successfully combined. It was registered by Ministry of agriculture, forestry and water management of Serbia Republic in 2013. This cultivar has wide adaptability and stability of yield that enable growing in different environments with optimal agricultural practice. On the base of technological quality this cultivar belongs to the second quality class, A2 farinograph subgroup and second technological group.

  14. Use of multiline cultivars and cultivar mixtures for disease management.

    Science.gov (United States)

    Mundt, C C

    2002-01-01

    The usefulness of mixtures (multiline cultivars and cultivar mixtures) for disease management has been well demonstrated for rusts and powdery mildews of small grain crops. Such mixtures are more useful under some epidemiological conditions than under others, and experimental methodology, especially problems of scale, may be crucial in evaluating the potential efficacy of mixtures on disease. There are now examples of mixtures providing both low and high degrees of disease control for a wide range of pathosystems, including crops with large plants, and pathogens that demonstrate low host specificity, or are splash dispersed, soilborne, or insect vectored. Though most analyses of pathogen evolution in mixtures consider static costs of virulence to be the main mechanism countering selection for pathogen complexity, many other potential mechanisms need to be investigated. Agronomic and marketing considerations must be carefully evaluated when implementing mixture approaches to crop management. Practical difficulties associated with mixtures have often been overestimated, however, and mixtures will likely play an increasingly important role as we develop more sustainable agricultural systems.

  15. Effects of tomato juices (Lycopersicum esculentum Mill) consumption on plasma lycopene levels of male light smokers

    OpenAIRE

    Ida Gunawan; Sri S. Sudardjat; Septelia I. Wanandi

    2004-01-01

    The study was an experimental study with pre- and post-test design, to evaluate the effects of tomato juices (Lycopersicum esculentum Mill) consumption on plasma lycopene level. Blood sample of 27 subjects with average age of 34.70 ± 5.74 years old, moderate education level (85.2%), experienced direct contact with working environmental pollutant (40.74%) and smoked kretek cigarettes (77.78%) were given treatment with 350 gram/day of tomato juices (Lycopersicum esculentum Mill) which had been ...

  16. �Saruman� Apple Cultivar

    Directory of Open Access Journals (Sweden)

    Radu SESTRAS

    2010-06-01

    Full Text Available The apple cultivar Saruman was obtained at Fruit Research Station Cluj, Romania (FRS Cluj, and homologated in 2007. Saruman was released by hybridization between Cluj III-VI-5-26 selection (Parmain dOr, open pollinated and NJ 46. The trees are vigorous, spreading shape, and with medium crop yield. The fruits have large size, conic shape and mostly red (purple coloration; they have white flesh with a sweet, crisp, aromatic flavour and low acidity. Fruits become ripe in the last decade of August, first decade of September and the fruits are proper for dessert and well suited for cooking, applesauce, cider, pies.

  17. Isolasi Dan Identifikasi Terpenoid dari Fraksi n-Butanol Herba Lampasau (Diplazium esculentum Swartz

    Directory of Open Access Journals (Sweden)

    Maria Dewi Astuti

    2017-03-01

    Full Text Available Abstrak Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa kimia yang diisolasi dari fraksi n-butanol ekstrak metanol herba lampasau (Diplazium esculentum Swartz. Ekstrak metanol diperoleh secara maserasi dan difraksinasi berturut-turut denganpetroleum eter, etil asetat, dan n-butanol. Fraksi n­-butanol difraksinasidengan kromatografi kolom dengan fase diam silika gel dihasilkan fraksi A, B, C, dan D. Fraksi B dimurnikan dengan kromatografi lapis tipis preparatif pada silika geldihasilkan isolat B1. Isolat B1 berupa padatan tidak berwarna danberfluoresensi putih di bawah lampu UV 366 nm. Panjang gelombang maksimum pada spektra UV  isolat B1 adalah 225 nm dan 272.5 nm yang menunjukkan adanya ikatan rangkap tak terkonjugasi. Spektra IR isolat B1 menunjukkan adanya gugus C=C, –OH, C=O lakton, –CO, C–H ulur, dan C–H tekuk. Spektra 1H-NMR isolat B1 menunjukkan sinyal proton pada ikatan rangkap, proton –OH, proton pada –CH2 yang terikat atom oksigen, serta proton gugus metil –CH3. Berdasarkan data spektra UV, IR, dan 1H-NMR maka isolat B1 disarankan sebagai turunan senyawa triterpenoid hopan-lakton. Kata kunci : diplazium esculentum Swartz, fraksi n-butanol, triterpenoid hopan-lakton  Abstract The research  aims to identify chemical compounds isolated fromn-butanol fraction methanol extract of lampasau herbs (Diplazium esculentum Swartz. The methanol extract was obtained by maceration and fractioned by petroleum ether, ethyl acetate, andn-butanol. N-butanol fraction was fractionated using column chromatography on silica gel produced fractions A, B, C, and D. Fraction B was purified by preparative thin layer chromatography on silica gel produced isolate B1. Isolate B1was colorless solid and has white fluorescent under UV lamp 366 nm. The maximum wavelength on UV spectra of B1 are 225 nm and 272,5 nm indicates the unconjugated double bond. IR spectra of B1 showed the vibration of C=C, –OH, C=O lactone, –CO, C

  18. Role of nano-SiO2 in germination of tomato (Lycopersicum esculentum seeds Mill.)

    OpenAIRE

    Manzer H. Siddiqui; Al-Whaibi, Mohamed H.

    2013-01-01

    Agricultural biotechnology is very familiar with the properties of nanomaterial and their potential uses. Therefore, the present experiment was conducted to test the beneficial effects of nanosilicon dioxide (nSiO2: size- 12 nm) on the seed germination of tomato (Lycopersicum esculentum Mill. cv Super Strain B). Application of nSiO2 significantly enhanced the characteristics of seed germination. Among the treatments, 8 g L−1 of nSiO2 improved percent seed germination, mean germination time, s...

  19. RESPONSE OF CABBAGE CULTIVARS TO BLACK ROT INFECTION

    African Journals Online (AJOL)

    Yield stability of white cabbage (Brassica oleracea var. capitata) cultivars were ... and stable cultivar(s) with adequate resistance to black rot caused by ..... Principles and Procedures of Statistics: ... black rot development in introduced cabbage.

  20. Genetic Diversity within Cultivar Population and among Different Cultivars of Common Buckwheat%甜荞品种内与品种间的遗传多样性研究

    Institute of Scientific and Technical Information of China (English)

    王忠景; 冯佰利; 柴岩; 胡银岗

    2009-01-01

    以9个甜荞品种为材料,1个苦荞品种为对照,每个品种随机取10粒种子发芽,提取基因组DNA,采用长随机引物和内含子切接点引物的PCR分子标记技术,对甜荞品种内和品种间的遗传多样性进行了分析.结果表明:(1)所使用的引物均能在 9份甜荞品种和1份苦荞品种中稳定扩增出条带,共获得了45条条带,其中多态性条带42条,占总数的93.3%;(2)甜荞同一品种内存在一定的遗传差异,但遗传距离较小,大部分不同甜荞品种间的遗传差异大于其品种内的遗传差异.因而,在甜荞品种鉴定和遗传多样性研究中,通过品种内适当混合取样可以较好地代表和反映甜荞品种特性.%Common buckwheat (F.esculentum) is an allogamy crop,which is entomophilous heterogony.Revealing the genetic diversity within cultivar population has great theoretical and practical significance for the establishment of the sampling strategy in its genetic diversity study.Nine common buckwheat varieties were used as materials,and one tartary buckwheat variety was as control in this study.Ten seeds of each cultivar were randomly picked for seedling germination and genomic DNA extraction.PCR with intron-splice junction primers and long random primers was employed to reveal the genetic diversity within cultivar population and among cultivars of common buckwheat.The results showed:(1)All of the primers used could amplify stable bands in 9 common buckwheat cultivars and 1 tartary buckwheat cultivar,the total bands amplified were 45,of which 42 bands were polymorphic,accounting for 93.3% of total bands;(2)There were differences between different individuals of the same common buckwheat cultivar,but the difference within the cultivar population was much smaller than that among different cultivars.Therefore,taking mixed sample of appropriate seedlings of one cultivar could reflect the characteristics of the cultivar in the germplasm idenfication and genetic diversity study

  1. CULTIVAR IMPACT ON THE CHEMICAL CONTENT AND GRAIN TECHNOLOGICAL QUALITIES OF SOME DURUM WHEAT CULTIVARS

    Directory of Open Access Journals (Sweden)

    Tanko Kolev

    2011-11-01

    Full Text Available A comparative experiment was carried out with five Bulgarian and five foreign durum wheat cultivars. The aim of the experiment was to determine the chemical content and grain technological quality of some Bulgarian and foreign durum wheat cultivars grown under the agriecological conditions of Southern Bulgaria. The Vazhod cultivar proved to give the highest durum wheat grain yield, followed by Beloslava cultivar. Out of the foreign durum wheat cultivars the Durumko was notable for its higher productivity. The crude protein content in the grain was highest in Zagorka, Yavor and Yukon. The highest yield of gluten was reported in Beloslava, Vazhod and Zagorka.

  2. Introgression of High Yield Genes from Lycopersicon hirsutum acc. LA1777 Using CAPS Marker

    Institute of Scientific and Technical Information of China (English)

    LI Hong; WANG Xiao-xuan; SONG Ming; GAO Jian-chang; GUO Yan-mei; ZHU De-wei; DAI Shan-shu; DU Yong-chen

    2007-01-01

    The idea behind this study is to show that using high yield genes from a wild tomato can enrich tomato breeding resources and accelerate tomato breeding programs. In this study, the near-isogenic line TA1229 containing a 24-cM introgression at the bottom of chromosome 1 from Lycopersicon acc. LA1777, affects several higher yield traits. The TA1229 × 9706 BC1population was analyzed by marker-assisted selection and the traits of the population were evaluated. Twenty-three recombinant individuals that carried a shorter segment than TA1229 were obtained. Among them, 16 lines with the chromosome 1 recombinant segment can increase tomato yield and a QTL affecting yield was found between TG53 and TG158. Sixteen recombinant lines are useful to improve the tomato variety.

  3. Cannabis - from cultivar to chemovar.

    Science.gov (United States)

    Hazekamp, A; Fischedick, J T

    2012-01-01

    The medicinal use of Cannabis is increasing as countries worldwide are setting up official programs to provide patients with access to safe sources of medicinal-grade Cannabis. An important question that remains to be answered is which of the many varieties of Cannabis should be made available for medicinal use. Drug varieties of Cannabis are commonly distinguished through the use of popular names, with a major distinction being made between Indica and Sativa types. Although more than 700 different cultivars have already been described, it is unclear whether such classification reflects any relevant differences in chemical composition. Some attempts have been made to classify Cannabis varieties based on chemical composition, but they have mainly been useful for forensic applications, distinguishing drug varieties, with high THC content, from the non-drug hemp varieties. The biologically active terpenoids have not been included in these approaches. For a clearer understanding of the medicinal properties of the Cannabis plant, a better classification system, based on a range of potentially active constituents, is needed. The cannabinoids and terpenoids, present in high concentrations in Cannabis flowers, are the main candidates. In this study, we compared cultivars obtained from multiple sources. Based on the analysis of 28 major compounds present in these samples, followed by principal component analysis (PCA) of the quantitative data, we were able to identify the Cannabis constituents that defined the samples into distinct chemovar groups. The study indicates the usefulness of a PCA approach for chemotaxonomic classification of Cannabis varieties.

  4. Effects of Soybean Cultivars on Soymilk Quality

    Directory of Open Access Journals (Sweden)

    Aziadekey, M.

    2001-01-01

    Full Text Available Soymilk was prepared from twelve soybean cultivars grown under the same environmental conditions to evaluate their effects on soymilk characteristics. Significant correlations were observed between the Chemical composition of the seeds and the resultant soymilk. Soymilk solids were significantly affected by seed size and seed phosphorus contents. Cultivars with dark hilum produced soymilk with less attractive colour.

  5. Complete Chloroplast Genome Sequence of Tartary Buckwheat (Fagopyrum tataricum and Comparative Analysis with Common Buckwheat (F. esculentum.

    Directory of Open Access Journals (Sweden)

    Kwang-Soo Cho

    Full Text Available We report the chloroplast (cp genome sequence of tartary buckwheat (Fagopyrum tataricum obtained by next-generation sequencing technology and compared this with the previously reported common buckwheat (F. esculentum ssp. ancestrale cp genome. The cp genome of F. tataricum has a total sequence length of 159,272 bp, which is 327 bp shorter than the common buckwheat cp genome. The cp gene content, order, and orientation are similar to those of common buckwheat, but with some structural variation at tandem and palindromic repeat frequencies and junction areas. A total of seven InDels (around 100 bp were found within the intergenic sequences and the ycf1 gene. Copy number variation of the 21-bp tandem repeat varied in F. tataricum (four repeats and F. esculentum (one repeat, and the InDel of the ycf1 gene was 63 bp long. Nucleotide and amino acid have highly conserved coding sequence with about 98% homology and four genes--rpoC2, ycf3, accD, and clpP--have high synonymous (Ks value. PCR based InDel markers were applied to diverse genetic resources of F. tataricum and F. esculentum, and the amplicon size was identical to that expected in silico. Therefore, these InDel markers are informative biomarkers to practically distinguish raw or processed buckwheat products derived from F. tataricum and F. esculentum.

  6. Complete Chloroplast Genome Sequence of Tartary Buckwheat (Fagopyrum tataricum) and Comparative Analysis with Common Buckwheat (F. esculentum).

    Science.gov (United States)

    Cho, Kwang-Soo; Yun, Bong-Kyoung; Yoon, Young-Ho; Hong, Su-Young; Mekapogu, Manjulatha; Kim, Kyung-Hee; Yang, Tae-Jin

    2015-01-01

    We report the chloroplast (cp) genome sequence of tartary buckwheat (Fagopyrum tataricum) obtained by next-generation sequencing technology and compared this with the previously reported common buckwheat (F. esculentum ssp. ancestrale) cp genome. The cp genome of F. tataricum has a total sequence length of 159,272 bp, which is 327 bp shorter than the common buckwheat cp genome. The cp gene content, order, and orientation are similar to those of common buckwheat, but with some structural variation at tandem and palindromic repeat frequencies and junction areas. A total of seven InDels (around 100 bp) were found within the intergenic sequences and the ycf1 gene. Copy number variation of the 21-bp tandem repeat varied in F. tataricum (four repeats) and F. esculentum (one repeat), and the InDel of the ycf1 gene was 63 bp long. Nucleotide and amino acid have highly conserved coding sequence with about 98% homology and four genes--rpoC2, ycf3, accD, and clpP--have high synonymous (Ks) value. PCR based InDel markers were applied to diverse genetic resources of F. tataricum and F. esculentum, and the amplicon size was identical to that expected in silico. Therefore, these InDel markers are informative biomarkers to practically distinguish raw or processed buckwheat products derived from F. tataricum and F. esculentum.

  7. Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture

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    Pratta Guillermo

    2000-01-01

    Full Text Available The phenotypic stability of morphometric traits in Lycopersicon spp. (stem perimeter at the base, middle and top, and number of flowers per cluster was measured by multivariate analysis through a progeny test in order to estimate the genetic stability of these traits. Principal components were calculated for two groups of Lycopersicon spp., non-regenerated plants and the progeny of regenerated plants. Analysis of variance was performed to support principal component analysis. Both groups presented similar eigenvalues and eigenvectors, while no significant differences were found between any of the traits studied. These results indicated that the phenotypic structure was the same among the progeny of regenerated and non-regenerated plants, so that no variation would occur in in vitro culture. Multivariate analysis proved to be an appropriate methodology for the measurement of the stability of morphometric traits after one regeneration cycle.

  8. �Saruman� Apple Cultivar

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    Radu SESTRAS

    2010-06-01

    Full Text Available The apple cultivar �Saruman� was obtained at Fruit Research Station Cluj, Romania (FRS Cluj, and homologated in 2007. �Saruman� was released by hybridization between �Cluj III-VI-5-26� selection (�Parmain d�Or�, open pollinated and �NJ 46�. The trees are vigorous, spreading shape, and with medium crop yield. The fruits have large size, conic shape and mostly red (purple coloration; they have white flesh with a sweet, crisp, aromatic flavour and low acidity. Fruits become ripe in the last decade of August, first decade of September and the fruits are proper for dessert and well suited for cooking, applesauce, cider, pies.

  9. �Sauron� Apple Cultivar

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    Radu SESTRAS

    2010-06-01

    Full Text Available The new apple cultivar Sauron was obtained at Fruit Research Station Cluj, Romania (FRS Cluj, and homologated in 2007. Sauron was identified in open pollinated population of Cluj 3/83 selection [Cluj III-VI-5-26 selection (Parmain dOr, open pollinated x NJ 46]. The trees have moderate vigour, upright then spread, with moderate productivity. Fruits are medium to large, usually red, with a portion being greenish or yellow-green and purple red vertically striped. The fruit has good quality, being soft eating apple due to their lack of crispness. Quality indices include firmness, crispness and excellent flavour. Fruits become ripe between 15 August and 5 September and the fruits are good quality, being proper for dessert and industrialization (juice, applesauce, pies, and cider.

  10. �Sauron� Apple Cultivar

    Directory of Open Access Journals (Sweden)

    Radu SESTRAS

    2010-06-01

    Full Text Available The new apple cultivar �Sauron� was obtained at Fruit Research Station Cluj, Romania (FRS Cluj, and homologated in 2007. �Sauron� was identified in open pollinated population of �Cluj 3/83� selection [�Cluj III-VI-5-26� selection (�Parmain d�Or�, open pollinated x �NJ 46�]. The trees have moderate vigour, upright then spread, with moderate productivity. Fruits are medium to large, usually red, with a portion being greenish or yellow-green and purple red vertically striped. The fruit has good quality, being soft eating apple due to their lack of crispness. Quality indices include firmness, crispness and excellent flavour. Fruits become ripe between 15 August and 5 September and the fruits are good quality, being proper for dessert and industrialization (juice, applesauce, pies, and cider.

  11. Cultivar de soja BRS 66

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    Bertagnolli Paulo Fernando

    2002-01-01

    Full Text Available BRS 66, oriunda do cruzamento BR 83-147 x FT-Abyara, é indicada para cultivo no Rio Grande do Sul. Na média de 21 ambientes no RS, no período de 1993/94 a 1995/96, essa cultivar apresentou rendimento médio de grãos de 2.883 kg/ha. Apresenta resistência ao cancro-da-haste, à podridão-parda-da-haste, à mancha-olho-de-rã, à raça 1 de Phytophthora sojae, à pústula-bacteriana e ao oídio. Tem flor branca, pubescência marrom, tegumento da semente amarelo-fosco, hilo marrom e tipo de crescimento determinado. É de ciclo médio e apresenta estatura da planta de média a alta, com boa resistência ao acamamento.

  12. Caracterización y evaluación de recursos genéticos del genero Lycopersicon

    OpenAIRE

    Vallejo Cabrera Franco Alirio; Sierra Tulio Alberto; Osorio Erika Rocío

    1993-01-01

    Mediante los descriptores de IBPGR se caracterizaron y evaluaron 118 introducciones del género Lycopersicon, en el Centro Experimental de la Universidad Nacional Sede Palmira. Se utilizó el diseño experimental de Bloques al azar con dos repeticiones; la parcela experimental estuvo constituida por seis plantas. Se estudiaron nueve caracteres relacionados con la planta, diez caracteres relacionados con el fruto, componentes del rendimiento y presencia o ausencia de plagas y enfermedade...

  13. Dicty_cDB: CHS483 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ey sequence. 38 0.14 2 AI484136 |AI484136.1 EST248943 tomato resistant, Cornell Lycopersicon esculentum cDNA...490786.1 EST241494 tomato shoot, Cornell Lycopersicon esculentum cDNA clone cLEB3H9 similar to cinnamic acid...34.1 EST255034 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER8N1, mRNA sequence. 38 0.15

  14. Dicty_cDB: CHD312 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available mplete sequence. 42 0.056 6 AI484136 |AI484136.1 EST248943 tomato resistant, Cornell Lycopersicon esculentum...6 |AI490786.1 EST241494 tomato shoot, Cornell Lycopersicon esculentum cDNA clone cLEB3H9 similar to cinnamic...I773934.1 EST255034 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER8N1, mRNA sequence. 38

  15. Application of protein-phenolic based coating on tomatoes (Lycopersicum esculentum Aplicação de coberturas proteicas e fenólicas em tomates (Lycopersicum esculentum

    Directory of Open Access Journals (Sweden)

    Eliane Pereira Cipolatti

    2012-09-01

    Full Text Available The aim of this study was to investigate the use of protein-phenolic based coating made from fermented rice bran on cherry tomatoes (Lycopersicum esculentum. Tests were performed with glycerol 3% (v/v, glycerol with protein-phenolic rice bran extract (5%, glycerol with protein-phenolic extract after 96 hours of fermentation (5%, and a control (without coating. The coated cherry tomatoes were kept at room temperature for 28 days. Mass loss, pH and acidity, total soluble solids, and carotenoids were determined every 96 hours. The coating made from the biomass extract reduced the carotenoid and acidity levels in the fruits studied by 17 and 21.1%, respectively, compared to the control. The coating proved an efficient barrier to water vapor with mass loss of 57% less than the control suggesting that it can be used as an alternative for vegetable tissue conservation.Este trabalho teve como objetivo estudar a utilização de películas, à base de compostos proteicos e fenólicos provenientes de farelo de arroz fermentado, em tomates (Lycopersicum esculentum. Foram realizados testes com: glicerol 3% (v/v; glicerol com extrato fenólico e proteico do farelo de arroz (5%; glicerol com extrato fenólico e proteico da biomassa gerada em 96 hours (5%, e um controle (sem a película. Os tomates revestidos foram mantidos à temperatura ambiente durante 28 dias, sendo determinados, a cada 96 horas, os seguintes aspectos: a perda de massa, o pH e a acidez, os sólidos solúveis totais e os carotenoides. A película elaborada com os extratos da biomassa reduziu os níveis de carotenoides e acidez dos frutos estudados em 17 e 21,1%, respectivamente, em relação ao controle. A película também foi eficiente como barreira ao vapor de água; assim, com perda de massa 57% inferior à do controle, sugere-se que esta poderá ser utilizada como alternativa para conservação desse tecido vegetal.

  16. Main: ERELEE4 [PLACE

    Lifescience Database Archive (English)

    Full Text Available t) of tomato (L.e.) E4 and carnation GST1 genes; GST1 is related to senescence; Found in the 5'-LTR region o...; tomato (Lycopersicon esculentum); carnation (Dianthus caryophyllus); Lycopersicon chilense; AWTTCAAA ...

  17. Methyl de-esterification as a major factor regulating the extent of pectin depolymerization during fruit ripening: a comparison of the action of avocado (Persea americana) and tomato (Lycopersicon esculentum) polygalacturonases.

    Science.gov (United States)

    Wakabayashi, Kazuyuki; Hoson, Takayuki; Huber, Donald J

    2003-06-01

    Pectinmethylesterase (PME, EC 3.2.1.11) and polygalacturonase (PG, EC 3.2.1.15) are known to operate in tandem to degrade methylesterified polyuronides. In this study, PGs purified from tomato and avocado fruit were compared in terms of their capacity to hydrolyze water-soluble polyuronides from avocado before and following enzymic or chemical de-esterification. When assayed using polygalacturonic acid or polyuronides from avocado fruit, the activity of PG from tomato fruit was 3-4 times higher than that from avocado fruit. High molecular mass, low methylesterified (33%) water-soluble polyuronides (WSP) from pre-ripe avocado fruit (day 0) were partially depolymerized upon incubation with purified avocado and tomato PGs. In contrast, middle molecular mass, highly methylesterified (74%) WSP from day 2 fruit were largely resistant to the action of both PGs. PME or weak alkali treatment of highly methylesterified WSP decreased the methylesterification values to 11 and 4.5%, respectively. Treatment of de-esterified WSP with either avocado or tomato PGs caused extensive molecular mass downshifts, paralleling those observed during avocado fruit ripening. Although PME and PG are found in many fruits, the pattern of depolymerization of native polyuronides indicates that the degree of cooperativity between these enzymes in vivo differs dramatically among fruits. The contribution of PME to patterns of polyuronide depolymerization observed during ripening compared with physically compromised fruit tissues is discussed.

  18. PATRONES ELECTROFORÉTICOS DE PEROXIDASAS, CATALASAS, SUPERÓXIDO DISMUTASAS Y PROTEINAS TOTALES EN PLÁNTULAS DE TOMATE (Lycopersicon esculentum Mill SOMETIDAS A ESTRÉS DE TEMPERATURAS

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    Marilyn Florido

    2002-01-01

    Full Text Available El presente trabajo se desarrolló con el objetivo de detectar perfiles electroforéticos polimórficos, que puedan ser utilizados como marcadores genéticos para asistir la selección de accesiones de tomate tolerantes al calor. Para el desarrollo del trabajo se efectuó un análisis electroforético de peroxidasas, catalasas, superóxido dismutasas y proteínas totales de muestras controles y tratadas a 40°C durante 90 min. de cuatro accesiones con diferente grado de tolerancia a altas temperaturas: Nagcarlang, L-10-3, Campbell-28 y HC 3880. Se pudo observar que los patrones isoenzimáticos de peroxidasas y catalasas no se afectaron por el estrés de calor; no obstante, los perfiles electroforéticos de isoenzimas superóxido dismutasas mostraron una disminución de actividad en las accesiones Campbell-28 y HC 3880, susceptibles al calor con respecto al control. Se evidenció además la inducción de una banda de 100 kDa, ante la presencia del estrés de temperatura en las cuatro accesiones evaluadas.

  19. The application of high-concentration short-time chlorine dioxide treatment for selected specialty crops including Roma tomatoes (Lycopersicon esculentum), cantaloupes (Cucumis melo ssp. melo var. cantaloupensis) and strawberries (Fragaria×ananassa).

    Science.gov (United States)

    Trinetta, V; Linton, R H; Morgan, M T

    2013-06-01

    The effects of high-concentration short-time chlorine dioxide (ClO2) gas treatment on food-borne pathogens inoculated onto the surface of tomatoes, cantaloupes, and strawberries were studied. Produce were spot-inoculated with a mixture of Salmonella enterica (serotypes Montevideo, Javiana and Baildon), Escherichia coli O157:H7 (serotypes 204 P, EDL 933 and C792) or Listeria monocytogenes (serotypes Scott A, F 5069 and LCDC 81-861), and treated with ClO2 gas at 10 mg/l for 180 s. After ClO2 gas treatment, surviving populations were determined and shelf-life studies were conducted (microbial spoilage population, change in color and overall appearance). Significant microbial reduction (p 0.05). Results obtained suggest the potential use of high-concentration short-time ClO2 gas treatment as an effective online pathogen inactivation technology for specialty crops in large-scale produce packing operations.

  20. Métodos étodos de controle de lepidópteros na cultura do tomateiro (Lycopersicon esculentum Mill. = Methods for caterpillars’ control in the culture of tomato

    Directory of Open Access Journals (Sweden)

    Anatoli Lebedenco

    2007-07-01

    Full Text Available Foram avaliados 5 métodos de controle das lagartas que atacam a cultura do tomateiro: método convencional de aplicação de produtos fitossanitários; manejo integrado de pragas (MIP com produtos sintéticos; MIP com o emprego de extrato de Azadirachta indica (Nim à 5%; ensacamento das pencas de tomate e testemunha. Os métodos queutilizaram produtos fitossanitários (convencional e MIP foram eficientes no controle das lagartas do tomateiro e, em conseqüência, promoveram um aumento na produtividade da cultura em 156 e 165% respectivamente, em comparação à testemunha. O número de pulverizações foi reduzido em até 66,7%, quando se adotou o MIP comparado ao convencional. O tratamento MIP-Nim foi significativamente igual à testemunha, denotando não ser eficiente no controle das lagartas broqueadoras. O ensacamento das pencas de frutos de tomate consistiu em uma alternativa promissora na produção de frutos sem a presença de inseticidas.Five methods for the control of caterpillars, which attack the culture of tomato, were analyzed: the conventional method of application of phytosanitary products; the integrated pest management (IPM; the IPM with the use of Azadirachta indica extract at 5% (Neem; the sacking of the tomato fruit immediately after flowering, and the control plants (without any treatment against pest. The methods that used phytosanitary products (conventional and IPM efficiently controlled the caterpillars on the tomato plants and, as a consequence, promoted an increase in the productivity of the culture of 156 and 165% respectively, if compared with the control plants. The number of pulverizations had a reduction of 66.7% when the IPM was used compared to the conventional. The performance of the IPMNeem treatment was significantly the same as the control, denoting poor efficiency in controlling the borer caterpillars. The method of sacking the tomato fruit consisted of a promising alternative in the production without the presence of insecticides.

  1. Efecto de Bombus atratus (Hymenoptera: Apidae sobre la productividad de tomate (Lycopersicon esculentum Mill. bajo invernadero en la Sabana de Bogotá, Colombia

    Directory of Open Access Journals (Sweden)

    Cure José Ricardo

    2007-06-01

    Full Text Available

    En varios países se utilizan con éxito especies de abejorros del género Bombus para la polinización de tomate, reportándose incrementos de productividad hasta del 40%. En la Sabana de Bogotá existen varias especies nativas del género, por lo que se planteó examinar su potencial, desde el punto de vista de su cría en cautiverio y de su utilización como polinizadores de tomate y otras solanáceas. En este trabajo se evaluó el potencial de la especie nativa Bombus atratus como polinizador de tomate, mediante la introducción al cultivo de colonias criadas en cautiverio. Se comparó la autopolinización espontánea de la planta, frente a la obtenida con ayuda de las obreras de B. atratus. Los frutos visitados por ellas presentaron incrementos significativos para las variables ‘peso fresco del fruto’ (40,9%, ‘número de semillas’ (103,3%, ‘diámetro ecuatorial’ (14,3% y ‘proporción de lóculos bien desarrollados’ (42,2%. La relativa facilidad para la cría de esta especie en cautiverio, y el potencial demostrado en el aumento de productividad del tomate bajo invernadero, muestran la necesidad de profundizar en estos estudios en Colombia.

  2. Effects of cell suspension and cell·free culture filtrate of Pseudomonas aeruginosa in the control of root rot-root kont disease complex of tomato (Lycopersicon esculentum Mill.

    Directory of Open Access Journals (Sweden)

    I. A. Siddiqui

    2013-12-01

    Full Text Available The plant growth-promoting rhizobacterium Pseudomonas aeruginosa strain IE-6 was tested for antagonistic activity towards Meloidogyne javanica, the root-knot nematode and soilbome root-infecting fungi viz., Macrophomina phaseolina, Fusarium solani and Rhizoctonia solani under laboratory and greenhouse conditions. Cell-free culture filtrate of the bacterium caused significant reduction in egg hatching of M.javanica and inhibited radial growth of fungi in vitro. Cell-free culture filtrate also caused lyses in mycelium of F.solani. Under greenhouse conditions, soil drenches with the aqueous cell suspension or cell-free culture resulted in a considerable reduction in nematode population densities in soil and subsequent root-knot development due to M.javanica. In addition to nematode control, rhizobacterium application also inhibited root-infection caused by soilborne root~infecting fungi with significant enhancement of growth of tomato seedlings.

  3. CARACTERIZACIÓN MORFOAGRONÓMICA DE UNA COLECCIÓN DE VARIEDADES, LÍNEAS E HÍBRIDOS DE TOMATE (Lycopersicon esculentum Mill) EN CONDICIONES DE ORGANOPÓNICOS

    OpenAIRE

    Moya, C.; Marta Álvarez; Marilyn Florido; Dagmara Plana

    2003-01-01

    El presente trabajo se realizó con el objetivo de encontrar fuentes genéticas de interés para los programas de mejoramiento genético del tomate, encaminados a la obtención de variedades con características superiores a las tradicionales en las diferentes formas de producción agrícola. En el trabajo se muestra la caracterización morfoagronómica de 43 accesiones de tomate, entre las que se incluyen variedades, líneas e híbridos de crecimiento determinado e indeterminado. Como testigo se utiliza...

  4. Análisis del comportamiento de poblaciones segregantes en la primera generación de autogamia de híbridos comerciales de tomate Lycopersicon esculentum Mill Analysis of segregatinf F2 selfed populations in comercial tomato hybrids

    Directory of Open Access Journals (Sweden)

    Baena García Diosdado

    1992-12-01

    Full Text Available

    Se evaluaron las manifestaciones heteróticas en la primera generación de autogamia de cuatro híbridos comerciales de tomate. Los ensayos se realizaron en el Centro Experimental de la Universidad Nacional, Seccional Palmira. Los cuatro híbridos fueron seleccionados por su buen desempeño agronómico, los cuales se compararon con dos líneas avanzadas F6 y una variedad comercial de gran aceptación entre los agricultores. El trabajo se justificó debido a los altos costos de la semilla híbrida importada de tomate tipo-Milano, factor limitante para su empleo a nivel de pequeños agricultores. Las plántulas (F2, híbridos, líneas y variedad se sometieron al sistema de doble trasplante. Cuando las plántulas tenían 10 días se midieron descriptores cuantitativos como longitud del hipocotilo y área de las hojas cotiledonares. Antes de que las plantas se llevaran al vivero definitivo (campo, se hicieron lecturas individuales de altura de planta, diámetro basal, diámetro intermedio y longitud de la tercera hoja. Los resultados arrojaron rendimientos similares entre plantas F2 e híbridos, lo cual abre la posibilidad de usar la semilla F2 como una alternativa de bajo costo para los pequeños agricultores, comparada con la semilla híbrida.

    The heterotic expressions in the first generation of autogamy of 4 commercial tomato hybrids were evaluated. The trial was conducted at the Agronomy School of the National University - Palmira. The four hybrids were chosen for their good agronomic characteristics and were compared with a well known commercial variety. This is justificied for the high cost for the Milano-type tomate hybrid seed limits its use among small fanners. The plantlets (F2, Hybrids, lines and variety were transplanted twice. The experimental materials were evaluated using plantet quantitative traits such as hypocotil length and cotiledonary leaf area, at 10 days. Plant heigh, basal steam diameter, mean steam diameter and third leaf length were measured individually before the plants were set out in the field (at 30 days. The result indicate: (i F2 plant yields were similar to hybrids indicating the feasilibity of using tomato seed, (ii The economy of using F2 seed results in much lower costs, cornpared to hybrid seed. Small croppers could adopt this practice.

  5. LA BIOFERTILIZACIÓN CON RIZOBACTERIAS Y HONGOS MICORRÍZICOS ARBUSCULARES EN LA PRODUCCIÓN DE POSTURAS DE TOMATE (Lycopersicon esculentum Mill. Y CEBOLLA (Allium cepa L.. I. CRECIMIENTO VEGETATIVO

    Directory of Open Access Journals (Sweden)

    L. E. Pulido

    2003-01-01

    Full Text Available En áreas experimentales de la Universidad de Ciego de Ávila, sobre un suelo Ferralítico Rojo compactado eútrico y durante dos campañas hortícolas sucesivas, se estudió el efecto de la inoculación, simple y combinada, mediante recubrimiento de las semillas y prescindiendo de la fertilización mineral, con cuatro y cinco especies, respectivamente, de rizobacterias promotoras del crecimiento vegetal -RPCV- (Azospirillum brasilense, Azotobacter chroococcum, Burkholderia cepacia y Pseudomonas fluorescens y de hongos micorrízicos arbusculares -HMA- (Glomus clarum, G. fasciculatum, G. mosseae, G. agregatum y G. versiculiferum, sobre algunos indicadores del crecimiento de posturas de tomate y cebolla, tomando como criterio de evaluación la altura y la longitud radical de las plántulas. Los resultados mostraron que, para el tomate, la inoculación con Azospirillum brasilense, Azotobacter chroococcum y Burkholderia cepacia permitió obtener posturas de calidad equivalente a la alcanzada con la fertilización mineral, mientras que para la cebolla, solo Azospirillum brasilense y Azotobacter chroococcum lograron que las posturas tuvieran dicha calidad. En relación con la inoculación con HMA, las especies Glomus clarum, G. fasciculatum y G. mosseae, para ambos cultivos, produjeron posturas con valores de altura y longitud radical considerados óptimos. Con las coinoculaciones de RPCV + HMA se lograron posturas de calidad superior a la alcanzada con las mejores variantes de inoculación simple, destacándose las combinaciones de G. clarum y G. fasciculatum con A. brasilense para el tomate y de G. clarum y G. fasciculatum con A. chroococcum para la cebolla.

  6. The characterization of novel mycorrhiza-specific phosphate transporters from ¤Lycopersicon esculentum¤ and ¤Solanum tuberosum¤ uncovers functional redundancy in symbiotic phosphate transport in solanaceous species

    DEFF Research Database (Denmark)

    Nagy, F.; Karandashov, V.; Chague, W.

    2005-01-01

    Solanaceous species are among the >200 000 plant species worldwide forming a mycorrhiza, that is, a root living in symbiosis with soil-borne arbuscular-mycorrhizal (AM) fungi. An important parameter of this symbiosis, which is vital for ecosystem productivity, agriculture, and horticulture......, is the transfer of phosphate (Pi) from the AM fungus to the plant, facilitated by plasma membrane-spanning Pi transporter proteins. The first mycorrhiza-specific plant Pi transporter to be identified, was StPT3 from potato [Nature 414 (2004) 462]. Here, we describe novel Pi transporters from the solanaceous...... species tomato, LePT4, and its orthologue StPT4 from potato, both being members of the Pht1 family of plant Pi transporters. Phylogenetic tree analysis demonstrates clustering of both LePT4 and StPT4 with the mycorrhiza-specific Pi transporter from Medicago truncatula [Plant Cell, 14 (2002) 2413] and rice...

  7. Antibacterial and anticandidal activity of Tylosema esculentum (marama extracts

    Directory of Open Access Journals (Sweden)

    Amanda Minnaar

    2011-03-01

    Full Text Available Bean and tuber extracts of Tylosema esculentum (marama – an African creeping plant – were obtained using ethanol, methanol and water. Based on information that T. esculentum is used traditionally for the treatment of various diseases, the antibacterial and anticandidal effects of tuber and bean extracts were investigated. The antimicrobial activity of the extracts was tested on methicillin-resistant Staphylococcus aureus (MRSA, ATCC 6538, Mycobacterium terrae (ATCC 15755, Corynebacterium diphtheriae (clinical and Candida albicans (ATCC 2091. We performed the broth microdilution test for the determination of the minimum inhibitory concentration (MIC and a method to determine survival of microorganisms after in vitro co-incubation with the highest concentrations of T. esculentum extracts, followed by assessment of colony counts. Ethanol and methanol (phenolic bean extracts exhibited higher potency against bacteria and yeast than aqueous extracts. Marama bean seed coat crude ethanolic extract (MSCE and seed coat polyphenolic fractions, especially soluble-bound fraction (MSCIB, were highly antimicrobial against M. terrae, C. diphtheriae and C. albicans. All marama bean polyphenolic fractions, namely cotyledon acidified methanol fraction (MCAM, seed coat acidified methanol fraction (MSCAM, cotyledon insoluble-bound fraction (MCIB, seed coat insoluble-bound fraction (MSCIB, cotyledon-free polyphenolic fraction (MCFP and seed coat free polyphenolic fraction (MSCFP had high antimicrobial effects as shown by low respective MIC values between 0.1 mg/mL and 1 mg/mL. These MIC values were comparable to those of control antimicrobials used: amphotericin B (0.5 mg/mL and cesfulodin (0.1 mg/mL against C. diphtheriae, streptomycin (1.0 mg/mL and gentamicin (0.4 mg/mL against M. terrae, and amphotericin B (0.05 mg/mL against C. albicans

  8. Flower colours and pigments in tulip cultivars

    NARCIS (Netherlands)

    Eijk, van J.P.; Keulen, van H.A.; Dijk, van A.J.

    1987-01-01

    Aanvullend op eerder onderzoek, waarbij bijna 500 oude en nieuwe tulpencultivars op bloempigmenten werden geanalyseerd, worden in tabelvorm van diezelfde cultivars nu de bloemkleur en de relatieve hoeveelheden carotenoiden, delfinidine, cyanidine, pelargonidine en flavonolen aangegeven

  9. Phenotypic profiles of Armenian grape cultivars

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    Aroutiounian Rouben

    2015-01-01

    Full Text Available The conservation and sustainable use of grapevine biodiversity in Armenia is particularly important due to the large number of traditional local varieties. Being partially different from European grapevine gene pool, the material of Armenian local cultivars significantly contributes to the understanding of the genetic variation and is valuable source for target selection. During last years many Armenian grapevine cultivars have been already described and their genotypes determined, but some local varieties and wild accessions remain unidentified and their phenotypic characteristics overlooked. The comprehensive analysis of phenotypes is essential for research, including genetic association studies, cultivar evaluation and selection. The goal of our research was the phenotyping on the base of reproductive, carpological and analytical characteristics of 80 Armenian aboriginal and new grape cultivars. Description of phenotypic profiles is important step towards identification and conservation of genetic resources of Armenian grapes. In future, these data can be applied for breeding of improved grape varieties targeted to fresh consumption and wine production.

  10. Efeito de produtos fitossanitários utilizados na cultura do tomateiro (Lycopersicon esculentum Mill. sobre Trichogramma pretiosum Riley, 1879 nas gerações F1 e F2 em ovos de Anagasta kuehniella (Zeller, 1879 Effect of pesticides used on tomato crop (Lycopersicon esculentum Mill. on Trichogramma pretiosum Riley, 1879 in F1 and F2 generations on Anagasta kuehniella (Zeller, 1879 eggs

    Directory of Open Access Journals (Sweden)

    Geraldo Andrade Carvalho

    2003-04-01

    Full Text Available Duas populações de Trichogramma pretiosum Riley, 1879, provenientes de Alegre, ES = L9 e Venda Nova do Imigrante, ES = L10, foram submetidas aos principais produtos fitossanitários utilizados na cultura do tomateiro, após serem multiplicadas em ovos de Anagasta kuehniella (Zeller. Os ovos desse hospedeiro foram tratados e oferecidos ao parasitismo decorridas 0, 24 e 48 horas após o tratamento, e mantidos em câmaras climáticas a 25 ± 2ºC, UR de 60 ± 10% e fotofase de 14 horas. Os inseticidas deltametrina, abamectin e metamidofós, independentemente da linhagem de T. pretiosum, reduziram a longevidade de fêmeas da geração maternal. Triflumuron, clorfluazuron, benomil, clorotalonil, Bacillus thuringiensis, mancozeb, dimetomorf, tebufenozide, teflubenzuron, acefato, pirimicarbe, iprodiona, metamidofós e ciromazina não afetaram a capacidade de parasitismo de T. pretiosum na geração F1, independentemente da origem da população, e não afetaram a porcentagem de emergência dos indivíduos da geração F2, das duas linhagens.Two Trichogramma pretiosum Riley, 1879 populations from Alegre ES = L9 and Venda Nova do Imigrante, ES = L10 (Brazil were treated with the main pesticides used on tomato crop after reared on Anagasta kuehniella (Zeller eggs. The eggs were treated and submitted to parasitism on 0, 24 and 48 hours and kept in climatic chambers at 25 ± 2 ºC, 60 ± 10% RH and 14 hours photophase. The longevity of T. pretiosum (mother females, independently of strain, was decreased for insecticides deltamethrin, abamectin and methamidophos. No influence was detected in the parasitism capacity in F1 generation of T. pretiosum with the products triflumuron, chlorfluazuron, benomyl, chlorotalonil, Bacillus thuringiensis, mancozeb, dimetomorf, tebufenozide, teflubenzuron, acefate, pirimicarb, iprodione, methamidophos and ciromazine, and these compounds did not effected the emergence in F2 generation of this parasitoid, independently of the strain.

  11. Parental Selection in Rice Cultivar Improvement

    Institute of Scientific and Technical Information of China (English)

    Stanley Omar P B.SAMONTE; Rodante E.TABIEN; Lloyd T.WILSON

    2013-01-01

    The evaluation of rice (Oryza sativa L.) cultivars assists breeders in identifying useful trait relationships and in selecting parents as donors of specific traits.This study was conducted to compare long-grain rice cultivars using genotype x trait (GT) biplot analysis and determine potential donors of traits related to grain yield and quality.Seventeen cultivars in the 2005 and 2006 Uniform Regional Rice Nursery in Beaumont,Texas,USA were analyzed for 20 traits using GT biplot analysis.The GT biplots showed the diversity among cultivars with regards to yield-related traits.Cultivars recommended as donor parents were:Trenasse,Spring,Presidio,and Cocodrie for high grain yield and head rice rate;Trenasse and Presidio for semi-dwarfness; Banks for tall plant height; Wells for high flag leaf area,panicle weight,and number and mass of filled grains per panicle; Hidalgo for high tiller density; Francis for high number of spikelets per panicle; Spring and Trenasse for short flowering duration; Cheniere for more days to heading and maturity and Spring for less days; and Spring and Hidalgo for high grain weight.Breeders can use these cultivars with specific traits to increase grain yield and quality.

  12. Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye

    Science.gov (United States)

    Li, Cuiping; Yu, Huahua; Feng, Jinhua; Chen, Xiaolin; Li, Pengcheng

    2009-02-01

    In this study, several methods were compared for the efficiency to concentrate venom from the tentacles of jellyfish Rhopilema esculentum Kishinouye. The results show that the methods using either freezing-dry or gel absorption to remove water to concentrate venom are not applicable due to the low concentration of the compounds dissolved. Although the recovery efficiency and the total venom obtained using the dialysis dehydration method are high, some proteins can be lost during the concentrating process. Comparing to the lyophilization method, ultrafiltration is a simple way to concentrate the compounds at high percentage but the hemolytic activities of the proteins obtained by ultrafiltration appear to be lower. Our results suggest that overall lyophilization is the best and recommended method to concentrate venom from the tentacles of jellyfish. It shows not only the high recovery efficiency for the venoms but high hemolytic activities as well.

  13. Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye

    Institute of Scientific and Technical Information of China (English)

    YU Huahua; FENG Jinhua; CHEN Xiaolin; LI Pengcheng

    2009-01-01

    In this study, several methods were compared for the efficiency to concentrate venom from the tentacles of jellyfish Rhopilema esculentum Kishinouye. The results show that the methods using either freezing-dry or gel absorption to remove water to concentrate venom are not applicable due to the low concentration of the compounds dissolved. Although the recovery efficiency and the total venom obtained using the dialysis dehydration method are high, some proteins can be lost during the concentrating process. Comparing to the lyophilization method, ultrafiltration is a simple way to concentrate the compounds at high percentage but the hemolytic activities of the proteins obtained by ultrafiltration appear to be lower. Our results suggest that overall lyophilization is the best and recommended method to concentrate venom from the tentacles of jellyfish. It shows not only the high recovery efficiency for the venoms but high hemolytic activities as well.

  14. RESPONSE OF PHENOLIC METABOLISM INDUCED BY ALUMINIUM TOXICITY IN FAGOPYRUM ESCULENTUM MOENCH. PLANTS.

    Science.gov (United States)

    Smirnov, O E; Kosyan, A M; Kosyk, O I; Taran, N Yu

    2015-01-01

    Buckwheat genus (Fagopyrum Mill.) is one of the aluminium tolerant taxonomic units of plants. The aim of the study was an evaluation of the aluminium (50 μM effect on phenolic accumulation in various parts of buckwheat plants (Fagopyrum esculentum Moench). Detection of increasing of total phenolic content, changes in flavonoid and anthocyanin content and phenylalanine ammonia-lyase activity (PAL) were revealed over a period of 10 days of exposure to aluminium. The most significant effects of aluminium treatment on phenolic compounds accumulation were total phenolic content increasing (by 27.2%) and PAL activity rising by 2.5 times observed in leaves tissues. Received data could be helpful to understand the aluminium tolerance principles and relationships of phenolic compounds to aluminium phytotoxicity.

  15. Application of protein-phenolic based coating on tomatoes (Lycopersicum esculentum

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    Eliane Pereira Cipolatti

    2012-09-01

    Full Text Available The aim of this study was to investigate the use of protein-phenolic based coating made from fermented rice bran on cherry tomatoes (Lycopersicum esculentum. Tests were performed with glycerol 3% (v/v, glycerol with protein-phenolic rice bran extract (5%, glycerol with protein-phenolic extract after 96 hours of fermentation (5%, and a control (without coating. The coated cherry tomatoes were kept at room temperature for 28 days. Mass loss, pH and acidity, total soluble solids, and carotenoids were determined every 96 hours. The coating made from the biomass extract reduced the carotenoid and acidity levels in the fruits studied by 17 and 21.1%, respectively, compared to the control. The coating proved an efficient barrier to water vapor with mass loss of 57% less than the control suggesting that it can be used as an alternative for vegetable tissue conservation.

  16. Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye

    Institute of Scientific and Technical Information of China (English)

    FENG Jinhua; YU Huahua; LI Cuiping; XING Ronge; LIU Song; WANG Lin; CAI Shengbao; LI Pengcheng

    2009-01-01

    The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry. Four types of nematocysts were identified: mastigophores, euryteles, and atrichous and holotrichous isorhiza. SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa, and that glutamic acid was the main amino acid. A hemolytic activity assay showed that the activity of the nematocyst venom (RNV) was strongest in Tris-HCl buffer (50 mmol/L, pH 7.8, 5% glycerol, 0.5 mmol/L EDTA, 0.1 mol/L NaCl). The hemolytic activity was related to protein concentration and the HU_(50) against chicken erythrocytes was 0.91 μg/mL.

  17. Antioxidant activity of tartary (Fagopyrum tataricum (L.) Gaertn.) and common (Fagopyrum esculentum moench) buckwheat sprouts.

    Science.gov (United States)

    Liu, Chia-Ling; Chen, Yih-Shyuan; Yang, Joan-Hwa; Chiang, Been-Huang

    2008-01-01

    This study compared the differences of two types of buckwheat sprouts, namely, common buckwheat ( Fagopyrum esculentum Moench) and tartary buckwheat ( Fagopyrum tataricum (L.) Gaertn.), in general composition, functional components, and antioxidant capacity. The ethanol extracts of tartary buckwheat sprouts (TBS) had higher reducing power, free radical scavenging activity, and superoxide anion scavenging activity than those of common buckwheat sprouts (CBS). As for chelating effects on ferrous ions, CBS had higher values than TBS. Rutin was the major flavonoid found in these two types of buckwheat sprouts, and TBS was 5 fold higher in rutin than CBS. The antioxidant effects of buckwheat sprouts on human hepatoma HepG2 cells revealed that both of TBS and CBS could decrease the production of intracellular peroxide and remove the intracellular superoxide anions in HepG2 cells, but TBS reduced the cellular oxidative stress more effectively than CBS, possibly because of its higher rutin (and quercetin) content.

  18. Trace element water improves the antioxidant activity of buckwheat (Fagopyrum esculentum Moench) sprouts.

    Science.gov (United States)

    Liu, Chia-Ling; Chen, Yih-Shyuan; Yang, Joan-Hwa; Chiang, Been-Huang; Hsu, Cheng-Kuang

    2007-10-31

    Buckwheat (Fagopyrum esculentum Moench) was grown in trace element water (TEW) (100, 200, 300, 400, and 500 ppm) and deionized water (DIW) to evaluate whether the beneficial effects of trace elements on the antioxidant activity could be accomplished with the supplement of TEW. At 300 ppm, TEW significantly increased the Cu, Zn, Mn, and Fe contents in buckwheat sprout but not the Se content. However, the levels of rutin, isoorientin, vitexin, and isovitexin did not differ between buckwheat sprouts grown in TEW and DIW. The ethanolic extract from buckwheat sprout grown in 300 ppm of TEW showed higher ferrous ion chelating activity and inhibitory activity toward lipid peroxidation than that grown in DIW. The extract in the TEW group also enhanced intracellular superoxide dismutase activity and lowered reactive oxygen species and superoxide anion in the human Hep G2 cell. It was concluded that TEW could increase the antioxidant activities of buckwheat sprouts.

  19. RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon.

    Science.gov (United States)

    Miller, J C; Tanksley, S D

    1990-10-01

    Forty single-copy, nuclear probes of known chromosomal position were used to examine restriction fragment length polymorphism in the tomato genus Lycopersion. The probes were from three libraries: one cDNA, and two genomic libraries [Symbol: see text]ne genomic made with EcoRI and the other with PstI. Total DNA from 156 plants representing eight species was cut with five different restriction enzymes and scored in 198 probe-enzyme combinations. Genetic distances between accessions (populations) and species were calculated from the resultant restriction patterns and proportion of shared bands. Accessions belonging to the same species largely clustered together, confirming their current classification. However, one mountain accession, classified as L. peruvianum var. humifusum (LA2150), was sufficiently distinct from the other accessions of L. peruvianum that it may qualify as a separate species L. esculentum and L. pimpinellifolium were the least clearly differentiated, possibly reflecting introgressive hybridization, known to have been promoted by man in recent history. Dendrograms constructed from cDNA versus genomic clones were nearly identical in their general grouping of species. The dendrograms revealed two major dichotomies in the genus: one corresponding to mating behavior [self-compatible (SC) versus self-incompatible (SI) species] and the other corresponding to fruit color (red versus green-fruited species). The ratio of withinversus between-accession diversity was much lower for SC species, indicating that most of the diversity within these species exists between populations, rather than within populations. Overall, the amount of genetic variation in the SI species far exceeded that found in SC species. This result is exemplified by the fact that more genetic variation could be found within a single accession of one of the SI species (e.g., L. peruvianum) than among all accessions tested of any one of the SC species (e.g., L. esculentum or L

  20. Growth and Photosynthetic Pigment Accumulation in Lycopersicum esculentum Mill. in Response to Light and Nutrient Stress

    Directory of Open Access Journals (Sweden)

    Gideon Olarewaju OKUNLOLA

    2014-06-01

    Full Text Available This study aimed at determining the responses of some of the growth parameters of Lycopersicum esculentum to light and nutrient stress. The focus is equally orientated in determining the effect of light and nutrient stress on the photosynthetic pigment accumulation in plants. Tomato seeds were grown in pretreated sand and were watered with distilled water until the experimental culture was fully established. After this, the plants were transplanted into 60 plastic pots, with holes bored at the bottom to allow proper drainage of the excess water during the course of the experiment. The plants were divided into four groups of fifteen pots each. A group of plants was submitted to stress of nutrient lack by only administering 100 ml of complete nutrient solution, once every four days. The nutrient solution if applied daily was considered to be adequate for the plants. Another group of plants was lightly stressed, by placing them under shade, while adequate light was gotten in direct sunlight. Sampling was carried out at weekly intervals, starting from the seventh day after the first treatment. Plants were randomly picked from each of the four treatments. Three replicates were used for each parameter. The results showed that there was a reduction in photosynthetic pigment accumulation in the plants when both light and nutrient were limited. The data obtained from the study were first tested between normality and assumption of constant variance. A two-way analysis of variance (ANOVA was carried out considering both factors (light and nutrient as source's of variation to investigate the effects of full light and full nutrient (FLFN , full light and partial nutrient (FLPN, partial light and full nutrient (PLFN and partial light and partial nutrient (PLPN the parameters studied in Lycopersicum esculentum.

  1. Comparison of polyphenol oxidase expression in glandular trichomes of solanum and lycopersicon species.

    Science.gov (United States)

    Yu, H; Kowalski, S P; Steffens, J C

    1992-12-01

    Tetralobulate glandular trichomes are present on the foliage of many solanaceous species. Resistance of many of these species to insects is conditioned by the ability of trichomes to rupture upon contact and to rapidly polymerize their contents, resulting in entrapment of insects in hardened trichome exudate. In the wild potato, Solanum berthaultii, polymerization of trichome exudate is initiated by a soluble M(r) 59,000 polyphenol oxidase (PPO), which is a dominant protein constituent of the organ. PPOs, although ubiquitous in angiosperms, typically display great heterogeneity in molecular weight and are found at low levels in plant cells. Because of the unusually high accumulation and tissue-specific expression of the M(r) 59,000 PPO in S. berthaultii glandular trichomes, we analyzed trichome proteins of a number of Lycopersicon and Solanum species to assess the extent to which possession of the M(r) 59,000 PPO is conserved. Trichomes were collected manually and examined for PPO activity, immuno-cross-reactivity with S. berthaultiiM(r) 59,000 PPO, and protein content. In addition, N-terminal amino acid sequences were obtained for five trichome PPOs. All species analyzed possessed trichome PPOs similar in structure and level of expression to that of S. berthaultii. The relationship between sequences and structures of these conserved PPOs and the variable PPOs of leaf is discussed.

  2. Selenium fertilization alters the chemical composition and antioxidant constituents of tomato (Solanum lycopersicon L.).

    Science.gov (United States)

    Schiavon, Michela; Dall'acqua, Stefano; Mietto, Anna; Pilon-Smits, Elizabeth A H; Sambo, Paolo; Masi, Antonio; Malagoli, Mario

    2013-11-06

    Although selenium (Se) is a known anticarcinogen, little is known regarding how Se affects other nutritional qualities in crops. Tomato ( Solanum lycopersicon ) was supplied with 0-50 μM selenate and analyzed for elemental composition and antioxidant compounds. When supplied at low doses (5 and 10 μM) via the roots, Se stimulated the synthesis of phenolic compounds in leaves and reduced the levels of Mo, Fe, Mn, and Cu in roots. At higher doses (25 and 50 μM Se) leaf glutathione levels were 3-5-fold enhanced. Supply of selenate via foliar spray (0, 2, or 20 mg Se plant(-1)) resulted in Se-biofortified tomato fruits, with Se levels low enough not to pose a health risk. The Se-biofortified fruits showed enhanced levels of the antioxidant flavonoids naringenin chalcone and kaempferol and a concomitant decrease of cinnamic acid derivatives. Thus, tomato fruits can be safely enriched with Se, and Se biofortification may enhance levels of other neutraceutical compounds.

  3. CULTIVAR RELEASE-IPR 98: Rust-resistant dwarf arabica coffee cultivar for dense spacing

    Directory of Open Access Journals (Sweden)

    Tumoru Sera

    2008-01-01

    Full Text Available The IPR 98 coffee cultivar is a dwarf plant smaller than the Catuaí Vermelho IAC-81 cultivar, completelyresistant to Hemileia vastatrix Berk. et Br. with medium precocity in ripening and superior quality and high yields with semidense,dense and super dense spacing in lower temperature coffee regions of the state of Paraná.

  4. Cultivar identification and genetic relationship of pineapple (Ananas comosus) cultivars using SSR markers.

    Science.gov (United States)

    Lin, Y S; Kuan, C S; Weng, I S; Tsai, C C

    2015-11-25

    The genetic relationships among 27 pineapple [Ananas comosus (L.) Merr.] cultivars and lines were examined using 16 simple sequence repeat (SSR) markers. The number of alleles per locus of the SSR markers ranged from 2 to 6 (average 3.19), for a total of 51 alleles. Similarity coefficients were calculated on the basis of 51 amplified bands. A dendrogram was created according to the 16 SSR markers by the unweighted pair-group method. The banding patterns obtained from the SSR primers allowed most of the cultivars and lines to be distinguished, with the exception of vegetative clones. According to the dendrogram, the 27 pineapple cultivars and lines were clustered into three main clusters and four individual clusters. As expected, the dendrogram showed that derived cultivars and lines are closely related to their parental cultivars; the genetic relationships between pineapple cultivars agree with the genealogy of their breeding history. In addition, the analysis showed that there is no obvious correlation between SSR markers and morphological characters. In conclusion, SSR analysis is an efficient method for pineapple cultivar identification and can offer valuable informative characters to identify pineapple cultivars in Taiwan.

  5. Resistance of some commercial winter wheat cultivars to Tilletia tritici

    Directory of Open Access Journals (Sweden)

    Staletić Mirjana D.

    2007-01-01

    Full Text Available This paper deals with the resistance of twenty commercial winter wheat cultivars to common bunt causal agent (Tilletia tritici. Significant differences among the cultivars concerning the infection percent were observed, as well as the differences in the level of commercial cultivars' resistance to T. tritici. Most of the studied cultivars belonged to susceptible categories, and just few of them to the resistant ones. Cultivar Lasta was classified as highly resistant during the both investigation years in Kragujevac, while in Leposavić Lasta and Tiha were classified as resistant. The other studied cultivars were more or less susceptible.

  6. Allelopathic evaluation of ethanol extracts of Copaifera sabulicola on the initial development of Lactuca sativa, Lycopersicum esculentum, and Zea mays

    Directory of Open Access Journals (Sweden)

    Manoel Viana Linhares Neto

    2014-09-01

    Full Text Available This paper aimed to evaluate the allelopathic potential of ethanol extracts from stem and leaves of Copaifera sabulicola on seed germination and seedling growth of Lactuca sativa and Lycopersicum esculentum, as well as to evaluate the allelopathic effect of these extracts on seedling germination and growth of Zea mays, through bioassays under laboratory conditions. The extracts were tested at the concentrations 0, 250, 500, and 1,000 mg.L-1. We evaluated the germination percentage, germination average speed, germination speed index, and seedling average length. All parameters underwent variance analysis, and the mean values were compared by means of Scott-nott test p 0.05. We found out that, in L. sativa, the extract from stem promoted signicant stimuli on germination percentage and root growth, while the extract from leaves only stimulated root growth. In L. esculentum, the extract from stem promoted signicant stimulus on hypocotyl and root growth, in turn, the extract from leaves stimulated only root growth. In Z. mays, both extracts signicantly inhibited germination. Therefore, we found out the allelopathic potential of ethanol extracts of C. sabulicola on L. sativa and L. esculentum, as well as inhibitory allelopathic effect on Z. mays.

  7. Allelopathic evaluation of ethanol extracts of Copaifera sabulicola on the initial development of Lactuca sativa, Lycopersicum esculentum, and Zea mays

    Directory of Open Access Journals (Sweden)

    Manoel Viana Linhares Neto

    2014-05-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2014v27n3p23 This paper aimed to evaluate the allelopathic potential of ethanol extracts from stem and leaves of Copaifera sabulicola on seed germination and seedling growth of Lactuca sativa and Lycopersicum esculentum, as well as to evaluate the allelopathic effect of these extracts on seedling germination and growth of Zea mays, through bioassays under laboratory conditions. The extracts were tested at the concentrations 0, 250, 500, and 1,000 mg.L-1. We evaluated the germination percentage, germination average speed, germination speed index, and seedling average length. All parameters underwent variance analysis, and the mean values were compared by means of Scott-Knott test (p ≤ 0.05. We found out that, in L. sativa, the extract from stem promoted significant stimuli on germination percentage and root growth, while the extract from leaves only stimulated root growth. In L. esculentum, the extract from stem promoted significant stimulus on hypocotyl and root growth, in turn, the extract from leaves stimulated only root growth. In Z. mays, both extracts significantly inhibited germination. Therefore, we found out the allelopathic potential of ethanol extracts of C. sabulicola on L. sativa and L. esculentum, as well as inhibitory allelopathic effect on Z. mays.

  8. Cruzamientos interespecíficos de Lycopersicon spp. y estudios preliminares de la resistencia genética a cogollero, Scrobipalpula absoluta Interespecific hybrids of Lycopersicon spp and preliminares studies of genetic resistance to pinworns Scrobipalpula absoluta

    Directory of Open Access Journals (Sweden)

    Estrada Salazar Edgar Iván

    1992-12-01

    Full Text Available Entre 1990 y 1992 se realizó en Palmira y Candelaria, Valle del Cauca, Colombia, una investigación con las especies silvestres L. hirsutum y L. pimpinellifolium,  las variedades comerciales de L. esculentum cv. Santa Clara cv Angela gigante y cv tropic. Se obtuvieron híbridos interespecíficos, retrocruzas (BC y poblaciones F2, se utilizó la especie cultivada como progenitor femenino. La evaluación de la resistencia genética se efectuó con base a criterios asociados a número de yemas afectadas/planta, número de hojas afectadas/planta y número de ampollas/planta. Para cada población se generó un índice de resistencia que considera de manera integral las variables en estudio. La efectividad de cruzamiento en híbridos interespecíficos y  retrocruzamientos fue de 9% y 5% respectivamente. La germinación en semillas híbridas fue del 25% y en retrocruzamientos del 35%. Se encontraron plantas que expresaron resistencia a cogollero con valores de índice similar a la especie silvestre, tanto para la L. hirsutum (9 y L. pimpinellifolium (25. Existió asociación entre la presencia de tricomas y la expresión de resistencia en las plantas de los cruzamientos con L. hirsutum; sin embargo esto no ocurrió en los cruzamientos con L. pimpinellifolium.This work was carried out in Palmira and Candelaria during 1990 to 1992 to evaluate the genetic resistance to pinworn, the genotypes used were: wild species: L. hirsuhl1n and L. pimpinellifolium; commercial varieties; L. esculentum cv. Santa Clara, L. esculentum cv Angela gigante and L. esculentum cv. Tropic. The trial to obtain  the experimental populations; (interspecific hybrids, back crossings and F2 populations was done under field conditions, using the cultivated species as female parent. A resistance index was generated which integrated all variables in study. The results presented: a low effectivity of crossings in interspecific hybrids and back crossings with 9% and 5% respectively

  9. Cadmium accumulation in different pakchoi cultivars and screening for pollution-safe cultivars

    Institute of Scientific and Technical Information of China (English)

    Ying CHEN; Ting-qiang LI; Xuan HAN; Zhe-li DING; Xiao-e YANG; Ye-fei JIN

    2012-01-01

    The selection and breeding of pollution-safe cultivars (PSCs) is a practicable and cost-effective approach to minimize the influx of heavy metal to the human food chain.In this study,both pot-culture and field experiments were conducted to identify and screen out cadmium pollution-safe cultivars (Cd-PSCs) from 50 pakchoi (Brassica rapa L.ssp.chinensis) cultivars for food safety.When treated with 1.0 or 2.5 mg/kg Cd,most of the pakchoi cultlvars (>70%)showed greater or similar shoot biomass when compared with the control.This result indicates that pakchoi has a considerable tolerance to soil Cd stress.Cd concentrations in the shoot varied significantly (P<0.05) between cultivars:in two Cd treatments (1.0 and 2.5 mg/kg),the average values were 0.074 and 0.175 mg/kg fresh weight (FW),respectively.Cd concentrations in the shoots of 14 pakchoi cultivars were lower than 0.05 mg/kg FW.In pot-culture experiments,both enrichment factors (EFs) and translocation factors (TFs) of six pakchoi cultivars were lower than 1.0.The field studies further confirmed that the Hangzhouyoudonger,Aijiaoheiye 333,and Zaoshenghuajing cultivars are Cd-PSCs,and are therefore suitable for growth in low Cd-contaminated soils (≤1.2 mg/kg) without any risk to food safety.

  10. Capacidade produtiva de cultivares de berinjela

    Directory of Open Access Journals (Sweden)

    Antonini Antonio C. C.

    2002-01-01

    Full Text Available Determinou-se a capacidade produtiva de diferentes híbridos e cultivares de berinjela. Sete híbridos (Nápoli, Diamante Negro, Ciça, F-100, Super F-100, Kiko e F-2000 e duas cultivares de polinização aberta (Suzuki e Florida Market foram avaliados. O delineamento experimental foi em blocos ao acaso, constituído de nove tratamentos e três repetições. Cada repetição foi composta de oito plantas. As mudas foram plantadas no espaçamento 1,5m x 1,0m. A cultivar Suzuki produziu a maior quantidade de frutos por planta (27 frutos embora tenha apenas diferido significativamente da cultivar Flórida Market (13 frutos. Os híbridos testados apresentaram número de frutos variando de 20 a 25. Não foi verificada diferença significativa para o número de frutos de 1ª e 2ª categoria entre os materiais testados. Flórida Market e Kiko apresentaram a maior massa média do fruto (362,5 e 358,7 g, respectivamente, diferindo significativamente da cultivar Suzuki, que produziu frutos com a menor massa (178,9 g. As maiores produções por planta foram obtidas com o híbrido Kiko que diferiu significativamente da cultivar Suzuki. Foi verificado que os híbridos Kiko, Diamante Negro, Ciça e F-2000 apresentam potencial de produção semelhante ao Nápoli, atual padrão de mercado.

  11. CULTIVAR RELEASE-IAC-Alvorada and IAC-Diplomata: new common bean cultivars

    Directory of Open Access Journals (Sweden)

    Sérgio Augusto Morais Carbonell

    2008-01-01

    Full Text Available The aim of common bean breeding programs is the development of high-yielding cultivars, with multipledisease resistance and high technological and nutritional grain quality. Two cultivars, IAC-Alvorada with carioca grain, andIAC-Diplomata with black grain, meet these standards, according to the results of trials of Value for Cultivation and Use(VCU 2005/2006/2007 in São Paulo. The cultivars, developed by the Instituto Agronômico de Campinas, were registered bythe MAPA/RNC.

  12. Intensity of Tuta absoluta (Meyrick, 1917 (Lepidoptera: Gelechiidae and Liriomyza spp. (Diptera: Agromyzidae attacks on Lycopersicum esculentum Mill. Leaves Intensidade de ataque de Tuta absoluta (Meyrick, 1917 (Lepidoptera: Gelechiidae e de Liriomyza spp. (Diptera: Agromyzidae em folhas de Lycopersicum esculentum Mill.

    Directory of Open Access Journals (Sweden)

    Germano Leão Demolin Leite

    2004-02-01

    Full Text Available The objective of this study was to determine the effect of height of leaves in the canopy of plants, leaf organic compounds, concentrations of leaf nitrogen and potassium, leaf trichomes and cristalliferous idioblasts densities on the attack intensity of three tomato (Lycopersicon esculentum Mill. cv. Santa Clara crops by Tuta absoluta (Meyrick (Lepidoptera: Gelechiidae and Liriomyza spp. (Diptera: Agromyzidae, under field conditions. The experimental design was arranged in randomized blocks, with three replications, each being one tomato crop. Analysis of variance and the Tukey’s multiple range test (5% significance were used to test the effect of canopy height, trichome and cristalliferous idioblasts densities on the number of mines produced by T. absoluta and Liriomyza spp. and on the number of T. absoluta eggs. Pearson’s correlation (5% significance was used to evaluate the relationships between leaf organic compounds, leaf N and K concentrations, leaf trichome and cristalliferous idioblasts densities and the number of mines produced by T. absoluta and Liriomyza spp. and the number of T. absoluta eggs. Highest insect attack occurred in the final stage of the culture. A higher number of Liriomyza spp. mines/leaf was recorded in the lower (1.50 than in the upper (0.02 level of the tomato plants, the opposite was observed for the number of T. absoluta eggs/leaf (0.13 and 0.57, respectively. The number of T. absoluta mines/leaf concentrated more on the median (10.23 and apical regions (8.63 than on the basal (4.93. No significant effect of the trichomes and cristalliferous idioblasts densities of leaves was noted on T. absoluta and Liriomyza ssp. populations. Apparently, the terpenes affected oviposition of T. absoluta while leaf potassium affected Liriomyza spp. attack.Objetivou-se com este estudo determinar as influências da posição das folhas no dossel das plantas, dos compostos orgânicos foliares, das concentrações de nitrogênio e

  13. Response of broccoli and cabbage hybrid cultivars to clomazone

    Science.gov (United States)

    Clomazone herbicide (Command 3ME) is registered for cabbage in the U.S., but not for other cultivar groups within Brassica oleracea. Cabbage cultivars vary in clomazone tolerance, and recommended use rates can cause severe foliar chlorosis and yield reduction to susceptible cultivars. The objectiv...

  14. Distinguishing Nonpareil marketing group almond cultivars through multivariate analyses.

    Science.gov (United States)

    Ledbetter, Craig A; Sisterson, Mark S

    2013-09-01

    More than 80% of the world's almonds are grown in California with several dozen almond cultivars available commercially. To facilitate promotion and sale, almond cultivars are categorized into marketing groups based on kernel shape and appearance. Several marketing groups are recognized, with the Nonpareil Marketing Group (NMG) demanding the highest prices. Placement of cultivars into the NMG is historical and no objective standards exist for deciding whether newly developed cultivars belong in the NMG. Principal component analyses (PCA) were used to identify nut and kernel characteristics best separating the 4 NMG cultivars (Nonpareil, Jeffries, Kapareil, and Milow) from a representative of the California Marketing Group (cultivar Carmel) and the Mission Marketing Group (cultivar Padre). In addition, discriminant analyses were used to determine cultivar misclassification rates between and within the marketing groups. All 19 evaluated carpological characters differed significantly among the 6 cultivars and during 2 harvest seasons. A clear distinction of NMG cultivars from representatives of the California and Mission Marketing Groups was evident from a PCA involving the 6 cultivars. Further, NMG kernels were successfully discriminated from kernels representing the California and Mission Marketing Groups with overall kernel misclassification of only 2% using 16 of the 19 evaluated characters. Pellicle luminosity was the most discriminating character, regardless of the character set used in analyses. Results provide an objective classification of NMG almond kernels, clearly distinguishing them from kernels of cultivars representing the California and Mission Marketing Groups.

  15. Growth and development in Achimenes cultivars

    NARCIS (Netherlands)

    Vlahos, J.C.

    1991-01-01

    Achimenes is a herbaceous perennial of the Gesneriaceae family. Its origin is Central and South America. It has been cultivated as a pot plant since the Victorian era. Extensive hybridization has produced many attractive cultivars which have been Introduced in

  16. Blueberry Cultivars for Oregon (EC 1308)

    Science.gov (United States)

    This Extension publication gives an overview of the types of blueberries and the blueberry cultivars that might be grown in Oregon or elsewhere in the Pacific Northwest. Bush and berry characteristics, yield potential, and suitability for commercial or home garden production are given for over 30 bl...

  17. Growth and development in Achimenes cultivars.

    NARCIS (Netherlands)

    Vlahos, J.C.

    1991-01-01

    Achimenes is a herbaceous perennial of the Gesneriaceae family. Its origin is Central and South America. It has been cultivated as a pot plant since the Victorian era. Extensive hybridization has produced many attractive cultivars which have been Introduced in commercial floriculture both In the Uni

  18. Micropropagation of peach rootstocks and cultivars.

    Science.gov (United States)

    Balla, Ildikó; Mansvelt, Lucienne

    2013-01-01

    Peach (Prunus persica (L.) Batsch) is one of the most popular stone fruits, commercially produced largely in Mediterranean and, to a lesser extent, in continental climatic conditions. Several breeding programs with different aims release annually large numbers of new cultivars. Micropropagation offers a suitable method to provide the growers of sufficient quantities of rootstocks, as well as of pathogen-free planting material of old and new cultivars.An effective four-step micropropagation procedure for cultivar and rootstock production is described here, based on the use of modified MS and WPM media. The health status of the initial shoot tips is very important, also because the growth and proliferation rate of shoot cultures from virus-infected clones are generally very poor. Proliferation and elongation phases depend on the major macro-elements, as well as the content and ratio of plant growth regulators. It is important to grow the cultures at 22°C, as hyperhydricity may develop at higher temperatures. Although sucrose is the most common carbon source used during proliferation and rooting, for some peach cultivars and rootstocks the replacement of sucrose (10 g/L) with glucose (20 g/L) in the rooting medium improves the rooting and survival rates of plants in the acclimatization phase. The rooting rate of the rootstock "Cadaman" is improved with the chelated form of iron FeEDDHA at 150 mg/L. Rooted plants are acclimatized in greenhouse under high humidity conditions.

  19. Distribution of Glucosinolates in Brassica oleracea cultivars

    NARCIS (Netherlands)

    Castro, A; Aires, A; Rosa, E; Bloem, E; Stulen, I.; De Kok, LJ

    2004-01-01

    Glucosinolates are important secondary compounds, commonly found in Brassicaceae. To what extent these sulfur and nitrogen containing compounds contribute to the total sulfur pool was investigated. In various cultivars of Brassica oleracea viz. curly kale, pointed cabbage, red cabbage, savoy cabbage

  20. Substrates in the development potted gerbera cultivar

    Directory of Open Access Journals (Sweden)

    Fernanda Ludwig

    2015-11-01

    Full Text Available The gerbera is a prominent culture that has gained in the Brazilian market of flowers and ornamental plants and there is a great demand for adequate substrates for its production. This study was conducted to evaluate the growth and distribution of dry mass and nitrogen in potted gerbera, depending on the substrates and cultivars. Experimental design was randomized blocks in a 5x2 factorial arrangement (5 substrates and 2 cultivars. Red and Cherry cultivars were evaluated at 1, 15, 29, 43 and 50 days of acclimation (DAA to the leaves number, leaf area, dry mass of aerial parts (leaf and flower and accumulation of nitrogen. From 29 DAA, the inflorescence has established as the drain and has contributed significantly to the total dry mass. The accumulation of nitrogen was similar in vegetative and reproductive periods. The traits evaluated varied depending on the substrate used, influenced by physical and chemical properties of the same. The growth, dry matter and nitrogen distribution potted gerbera cultivars Cherry and Red are influenced by physical and chemical characteristics of substrates, with better results in substrates with bulk density of less than 530 kg m-3 and pH values between 5.5 and 6.8

  1. Yield potential of pigeon pea cultivars

    Science.gov (United States)

    Yield potential of twelve vegetable pigeon pea (Cajanus cajun) cultivars was evaluated at two locations in eastern Kenya during 2012 and 2013 cropping years. Pigeon pea pod numbers, seeds per pod, seed mass, grain yield and shelling percentage were quantified in three replicated plots, arranged in a...

  2. Características fisiológicas de microtomateiros fitocromo-mutantes

    OpenAIRE

    Melo,Hyrandir Cabral de; Castro,Evaristo Mauro de; Soares,Ângela Maria; Oliveira,Cynthia de; Ramos,Sílvio Júnio

    2009-01-01

    Objetivou-se, neste trabalho, caracterizar aspectos fisiológicos de microtomateiros (Lycopersicon esculentum P. Miller cv. Micro-Tom) fitocromo-mutantes. A cultivar Micro-Tom e os mutantes aurea (deficiente na biossíntese do cromóforo dos fitocromos), atroviolacea (atv) e high pigment1 (hp1;ambos superexpressam eventos mediados por fitocromos) foram cultivados em condições controladas de luz e temperatura e caracterizados no estágio de floração. O mutante hp1 obteve as maiores taxas de fotoss...

  3. Características fisiológicas, anatômicas e ultra estruturais de micro-tomateiros fitocromo-mutantes

    OpenAIRE

    Melo,Hyrandir Cabral de

    2014-01-01

    O uso de microtomateiros fitocromo-mutantes é uma estratégia para o estudo do papel dos fitocromos sobre o desenvolvimento das plantas. O objetivo deste trabalho foi caracterizar aspectos fisiológicos, anatômicos e ultra-estruturais em microtomateiros (Lycopersicon esculentum cv. Micro-Tom) fitocromo-mutantes. A cultivar Micro-Tom (MT) e os mutantes aurea (subexpressa fitocromos), atroviolacea (atv) e hp1 (ambos superexpressam eventos mediados por fitocromos) foram cultivados em condições con...

  4. Soluciones nutritivas diluidas para la producción de jitomate a un racimo

    OpenAIRE

    Teodoro Gómez-Hernández; Felipe Sánchez-del Castillo

    2003-01-01

    Este trabajo se realizó con el propósito de determinar qué tanto pueden disminuirse las concentraciones de los nutrimentos de la solución nutritiva que normalmente se usa en siembras comerciales para la producción hidropónica de jitomate (Lycopersicon esculentum Mill.) en invernadero, sin que se afecte el rendimiento ni la calidad de fruto. Se trabajó con el cultivar Solarset despuntado para dejar sólo un racimo por planta a una densidad de 24 plantas m-2 de superficie. El riego se manejó sin...

  5. Differential response of domestic and wild Lycopersicon species to chilling under low light : Growth, carbohydrate content, photosynthesis and the xanthophyll cycle

    NARCIS (Netherlands)

    Venema, JH; Posthumus, F; van Hasselt, PR; de Vries, Michal

    1999-01-01

    The response of five Lycopersicon species to 14 days moderate chilling at 10 degrees C under low light (75 mu mol m(-2) s(-1)) and subsequent recovery was examined by measurements on relative shoot growth rate, leaf dry matter and carbohydrate content, CO2-exchange and pigment composition. In additi

  6. Isolation, Characterization and Evaluation of Collagen from Jellyfish Rhopilema esculentum Kishinouye for Use in Hemostatic Applications

    Science.gov (United States)

    Cheng, Xiaochen; Liu, Chenguang

    2017-01-01

    Hemostat has been a crucial focus since human body is unable to control massive blood loss, and collagen proves to be an effective hemostat in previous studies. In this study, collagen was isolated from the mesoglea of jellyfish Rhopilema esculentum Kishinouye and its hemostatic property was studied. The yields of acid-soluble collagen (ASC) and pepsin-soluble (PSC) were 0.12% and 0.28% respectively. The SDS-PAGE patterns indicated that the collagen extracted from jellyfish mesoglea was type I collagen. The lyophilized jellyfish collagen sponges were cross-linked with EDC and interconnected networks in the sponges were revealed by scanning electron microscope (SEM). Collagen sponges exhibited higher water absorption rates than medical gauze and EDC/NHS cross-linking method could improve the stability of the collagen sponges. Compared with medical gauze groups, the blood clotting indexes (BCIs) of collagen sponges were significantly decreased (P < 0.05) and the concentration of collagen also had an influence on the hemostatic property (P < 0.05). Collagen sponges had an improved hemostatic ability compared to the gauze control in tail amputation rat models. Hemostatic mechanism studies showed that hemocytes and platelets could adhere and aggregate on the surface of collagen sponge. All properties make jellyfish collagen sponge to be a suitable candidate used as hemostatic material and for wound healing applications. PMID:28103327

  7. Tylosema esculentum (Marama Tuber and Bean Extracts Are Strong Antiviral Agents against Rotavirus Infection

    Directory of Open Access Journals (Sweden)

    Walter Chingwaru

    2011-01-01

    Full Text Available Tylosema esculentum (marama beans and tubers are used as food, and traditional medicine against diarrhoea in Southern Africa. Rotaviruses (RVs are a major cause of diarrhoea among infants, young children, immunocompromised people, and domesticated animals. Our work is first to determine anti-RV activity of marama bean and tuber ethanol and water extracts; in this case on intestinal enterocyte cells of human infant (H4, adult pig (CLAB and adult bovine (CIEB origin. Marama cotyledon ethanolic extract (MCE and cotyledon water extract (MCW without RV were not cytotoxic to all cells tested, while seed coat and tuber extracts showed variable levels of cytotoxicity. Marama cotyledon ethanolic and water extracts (MCE and MCW, resp. (≥0.1 mg/mL, seed coat extract (MSCE and seed coat water extract (MSCW (0.01 to 0.001 mg/mL, especially ethanolic, significantly increased cell survival and enhanced survival to cytopathic effects of RV by at least 100% after in vitro co- and pre-incubation treatments. All marama extracts used significantly enhanced nitric oxide release from H4 cells and enhanced TER (Ω/cm2 of enterocyte barriers after coincubation with RV. Marama cotyledon and seed coat extracts inhibited virion infectivity possibly through interference with replication due to accumulation of nitric oxide. Marama extracts are therefore promising microbicides against RV.

  8. Effect of vermicompost on growth, yield and nutrition status of tomato (Lycopersicum esculentum).

    Science.gov (United States)

    Azarmi, Rasool; Ziveh, Parviz Sharifi; Satari, Mohammad Reza

    2008-07-15

    An experiment was conducted to determine the effects of vermicompost on growth, yield and fruit quality of tomato (Lycopersicum esculentum var. Super Beta) in a field condition. The experiment was a randomized complete block design with four replications. The different rates of vermicompost (0, 5, 10 and 15 t ha(-1)) was incorporated into the top 15 cm of soil. During experiment period, fruits were harvested twice in a week and total yield were recorded for two months. At the end of experiment, growth characteristics such as leaf number, leaf area and shoot dry weights were determined. The results revealed that addition of vermicompost at rate of 15 t ha(-1) significantly (at p Vermicompost with rate of 15 t ha(-1) increased EC of fruit juice and percentage of fruit dry matter up to 30 and 24%, respectively. The content of K, P, Fe and Zn in the plant tissue increased 55, 73, 32 and 36% compared to untreated plots respectively. The result of our experiment showed addition of vermicompost had significant (p < 0.05) positive effects on growth, yield and elemental content of plant as compared to control.

  9. Vermicompost as a soil supplement to improve growth, yield and fruit quality of tomato (Lycopersicum esculentum).

    Science.gov (United States)

    Gutiérrez-Miceli, Federico A; Santiago-Borraz, Jorge; Montes Molina, Joaquín Adolfo; Nafate, Camerino Carlos; Abud-Archila, Miguel; Oliva Llaven, María Angela; Rincón-Rosales, Reiner; Dendooven, Luc

    2007-11-01

    The effects of earthworm-processed sheep-manure (vermicompost) on the growth, productivity and chemical characteristics of tomatoes (Lycopersicum esculentum) (c.v. Rio Grande) were investigated in a greenhouse experiment. Five treatments were applied combining vermicompost and soil in proportions of 0:1, 1:1, 1:2, 1:3, 1:4 and 1:5 (v/v). Growth and yield parameters were measured 85 days and 100 days after transplanting. Addition of vermicompost increased plant heights significantly, but had no significant effect on the numbers of leaves or yields 85 days after transplanting. Yields of tomatoes were significantly greater when the relationship vermicompost:soil was 1:1, 1:2 or 1:3, 100 days after transplanting. Addition of sheep-manure vermicompost decreased soil pH, titratable acidity and increased soluble and insoluble solids, in tomato fruits compared to those harvested from plants cultivated in unamended soil. Sheep-manure vermicompost as a soil supplement increased tomato yields and soluble, insoluble solids and carbohydrate concentrations.

  10. Growth and rutin production in hairy root cultures of buckwheat (Fagopyrum esculentum M.).

    Science.gov (United States)

    Lee, Sook-Young; Cho, Soo-In; Park, Min-Hee; Kim, Yong-Kyung; Choi, Jae-Eul; Park, Sang-Un

    2007-01-01

    Buckwheat (Fagopyrum esculentum Moench.) is a potentially important source of rutin, a natural flavonoid with antihyperglycemic, antihypertensive, and antioxidative properties. To examine in vitro production of rutin, we established a hairy root culture of buckwheat by infecting leaf explants with Agrobacterium rhizogenes R1000, and tested the growth conditions and rutin production rates of these cultures. Ten hairy root clones were established; their growth and rutin production rates ranged from 233 to 312 (mg dry wt per 30 mL flask, and 0.8 to 1.2 (mg/g dry wt), respectively. Clone H8, which had high growth and rutin production rates (312 mg dry wt per 30 mL flask and 1.2 mg/g dry wt, respectively), was selected for further experiments. H8 showed maximal growth and rutin content at 30 days in culture in MS medium. Of four tested culture media, half-strength MS medium was found to induce the highest levels of growth (378 mg dry wt per 30 mL flask) and rutin production (1.4 mg/g dry wt) by clone H8. In contrast, supplementation with auxins (0.1-1 mg/l IAA, IBA and NAA) increased the growth rate, but had no significant effect on rutin production by H8. Collectively, these findings indicate that hairy root cultures of buckwheat culture could be a valuable alternative approach for rutin production.

  11. Pengaruhh konsentrasi larutan gula terhadap karakteristik manisan kering tomat (Lycopersicum esculentum

    Directory of Open Access Journals (Sweden)

    WAWAN BUNTARAN

    2011-05-01

    Full Text Available Buntaran W, Astirin PA, Mahajoeno M. 20011. Pengaruh konsentrasi larutan gula terhadap karakteristik manisan kering tomat (Lycopersicum esculentum. Bioteknologi 8: 1-9. Tujuan penelitian ini untuk mempelajari pengaruh perendaman dalam larutan gula terhadap karakteristik manisan tomat kering dan untuk menetapkan konsentrasi larutan gula yang tepat sehingga dihasilkan manisan tomat kering dengan karakteristik yang baik. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok (RAK yang terdiri dari empat perlakuan dengan enam kali ulangan. Perlakuan yang digunakan adalah konsentrasi larutan gula dimana untuk kelompok A (40%, B (50%, C (60% dan D (70%, selama 18 jam. Variabel yang diamati adalah kandungan air, abu, vitamin C dan uji organoleptik meliputi rasa, warna, aroma serta uji tekstur. Data dianalisis menggunakan Anova (Analisis of Variance dilanjutkan dengan uji DMRT (Duncan Multiple Range Test. Hasil penelitiaan menunjukkan bahwa konsentrasi larutan gula berpengaruh terhadap kandungan air, kandungan abu, kandungan vitamin C, tekstur serta warna,rasa dan aroma manisan. Manisan tomat kering kelompok A (40% relatif lebih baik dengan kandungan air 24,20%%, kandungan abu 0,62% dan vitamin C 31,15 mg/100 g. Syarat mutu kandungan manisan kering buah-buahan maximal 25% (SII No.0718¬2003, kandunggan abu bahan makanan maximal 1,0% (SII 0272.90 dan dan kandungan vitamin C tidak banyak berkurang dimana pada tomat adalah 30-40 mg/100 g. Hasil uji organoleptik menunjukkan bahwa kelompok A (40% mendapat nilai tertinggi terhadap rasa (3,98, aroma (3,89 dan warna (3,98.

  12. Characterisation of the arabinose-rich carbohydrate composition of immature and mature marama beans (Tylosema esculentum).

    Science.gov (United States)

    Mosele, Minah M; Hansen, Ase S; Engelsen, Søren B; Diaz, Jerome; Sørensen, Iben; Ulvskov, Peter; Willats, William G T; Blennow, Andreas; Harholt, Jesper

    2011-08-01

    Marama bean (Tylosema esculentum) is an important component of the diet around the Kalahari Desert in Southern Africa where this drought resistant plant can grow. The marama bean contains roughly 1/3 proteins, 1/3 lipids and 1/3 carbohydrates, but despite its potential as dietary supplement little is known about the carbohydrate fraction. In this study the carbohydrate fraction of "immature" and "mature" marama seeds are characterised. The study shows that the marama bean contains negligible amounts of starch and soluble sugars, both far less than 1%. The cell wall is characterised by a high arabinose content and a high resistance to extraction as even a 6M NaOH extraction was insufficient to extract considerable amounts of the arabinose. The arabinose fraction was characterised by arabinan-like linkages and recognised by the arabinan antibody LM6 and LM13 indicating that it is pectic arabinan. Two pools of pectin could be detected; a regular CDTA (1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid) or enzymatically extractable pectin fraction and a recalcitrant pectin fraction containing the majority of the arabinans, of which about 40% was unextractable using 6M NaOH. Additionally, a high content of mannose was observed, possibly from mannosylated storage proteins.

  13. Tylosema esculentum (Marama) Tuber and Bean Extracts Are Strong Antiviral Agents against Rotavirus Infection.

    Science.gov (United States)

    Chingwaru, Walter; Majinda, Runner T; Yeboah, Sam O; Jackson, Jose C; Kapewangolo, Petrina T; Kandawa-Schulz, Martha; Cencic, Avrelija

    2011-01-01

    Tylosema esculentum (marama) beans and tubers are used as food, and traditional medicine against diarrhoea in Southern Africa. Rotaviruses (RVs) are a major cause of diarrhoea among infants, young children, immunocompromised people, and domesticated animals. Our work is first to determine anti-RV activity of marama bean and tuber ethanol and water extracts; in this case on intestinal enterocyte cells of human infant (H4), adult pig (CLAB) and adult bovine (CIEB) origin. Marama cotyledon ethanolic extract (MCE) and cotyledon water extract (MCW) without RV were not cytotoxic to all cells tested, while seed coat and tuber extracts showed variable levels of cytotoxicity. Marama cotyledon ethanolic and water extracts (MCE and MCW, resp.) (≥0.1 mg/mL), seed coat extract (MSCE) and seed coat water extract (MSCW) (0.01 to 0.001 mg/mL), especially ethanolic, significantly increased cell survival and enhanced survival to cytopathic effects of RV by at least 100% after in vitro co- and pre-incubation treatments. All marama extracts used significantly enhanced nitric oxide release from H4 cells and enhanced TER (Ω/cm(2)) of enterocyte barriers after coincubation with RV. Marama cotyledon and seed coat extracts inhibited virion infectivity possibly through interference with replication due to accumulation of nitric oxide. Marama extracts are therefore promising microbicides against RV.

  14. The Control of Broomrape (Orobanche aegyptiaca in Tomato (Lycopersicum esculentum Mill Farms

    Directory of Open Access Journals (Sweden)

    K. Shirdel

    2014-12-01

    Full Text Available Broomrape would have irreversible effects on tomatoes in all growth stages and the damage would be unrecoverable. In order to chemically control Orobanche spp with consumption of bio-fertilizer in tomato cultivation, an experiment as factorial based on complete randomized blocks design in three replications and 12 plots was carried out at a farm which is located in Mahabad city (Iran. In this experiment, the experimental factors were including: A- sulfosulfuron 35 g/ha in three levels (application, at 40 days after transplanting of seedlings, application at 60 days and after sample transplanting without any chemical spraying, B and C: bio- fertilizers, Barvar -2 and Nitrajin in two levels (application and non-application of bio-fertilizer, were taken place. The results showed that Sulfosulfuron treatment at the rate of 35 gr/ha, with- twice application of 40 and 60 days after transplanting of seedlings decreased the biomass of Orobanche aegyptiaca in surface level and also it was possible to decrease the biomass per tomato bush and Orobanche aegyptiaca biomass to amount of 75%, 57% and 60% respectively compare to the main treatment without applying any kind of spray application. The applied bio-fertilizer-2 decreased the amount of fruit and economical function of tomato. The applying phosphate biofertilizer-2 resulted in a decrease in economical function of Lycopersicum esculentum and number of ripen fruits. However none of the applied treatment was influential on diameter and weight of ripen fruits.

  15. CULTIVAR RELEASE-CD 115: Soft Wheat Cultivar for colder regions

    Directory of Open Access Journals (Sweden)

    Volmir Sergio Marchioro

    2008-01-01

    Full Text Available The cultivar CD 115 was developed by the Cooperativa Central de Pesquisa Agrícola (COODETEC and isrecommended for cultivation in the states of Rio Grande do Sul, Santa Catarina and Paraná. The cultivar is tolerant todiseases and pre-harvest sprouting. The mean grain yield in four years of evaluation was 3,420 kg ha-1, exceeding thecontrols by 7%.

  16. CULTIVAR RELEASE - BRSMG União: common bean cultivar with jalo grain for the state of Minas Gerais

    Directory of Open Access Journals (Sweden)

    Magno Antonio Patto Ramalho

    2012-12-01

    Full Text Available The bean cultivar BRSMG União as a new option of a bean cultivar with jalo grains for the state of Minas Gerais. The cultivar BRSMG União had an average grain yield of 9.8% above the mean of the controls (Jalo EEP 558 and BRS Radiante and was resistant to powdery-mildew.

  17. Screening of Carnation Cultivars for Resistance to Meloidogyne incognita.

    Science.gov (United States)

    Cho, M R; Kim, J Y; Song, C; Ko, J Y; Na, S Y; Yiem, M S

    1996-12-01

    A total of 33 carnation cultivars cultured in Korea were screened for resistance to the southern root-knot nematode, Meloidogyne incognita. Carnations were tested by either inoculating with 5,000 eggs or by transplanting into a mixture of bedding medium and soil infested with an average of 435 second-stage juveniles/300 cm(3) soil. Cultivars, Desio, Castelaro, Kappa, Rara, Izu Pink, Target, and Antalia were highly resistant to M. incognita. Twelve cultivars were moderately resistant, and the remaining 14 cultivars were susceptible. These results were similar to those obtained when the cultivars were subjected to field populations of the condition on a carnation farm.

  18. Dicty_cDB: SFH171 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available |AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA c...82264 |AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA se

  19. Dicty_cDB: AFO831 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 2, WORKING DRAFT SEQUENCE. 46 0.55 1 AI773934 |AI773934.1 EST255034 tomato resistant, Cornell...90786 |AI490786.1 EST241494 tomato shoot, Cornell Lycopersicon esculentum cDNA cl...136 |AI484136.1 EST248943 tomato resistant, Cornell Lycopersicon esculentum cDNA

  20. Dicty_cDB: FCL-AD22 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available sequence. 80 4e-11 1 AI782264 |AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDN... |AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA

  1. Dicty_cDB: AFI206 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K... AI782264 |AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRN

  2. Dicty_cDB: AFO228 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available mato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA ...I782264 |AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum

  3. Dicty_cDB: CFJ845 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 3754.1 EST321699 tomato flower buds 3-8 mm, Cornell University Lycopersicon esculentum cDNA clone cTOB13E3 5... EST402393 tomato root, plants pre-anthesis, Cornell University Lycopersicon esculentum cDNA clone cLEY19A14

  4. Copper-induced oxidative damage, antioxidant response and genotoxicity in Lycopersicum esculentum Mill. and Cucumis sativus L.

    Science.gov (United States)

    İşeri, Özlem Darcansoy; Körpe, Didem Aksoy; Yurtcu, Erkan; Sahin, Feride Iffet; Haberal, Mehmet

    2011-09-01

    Adequate copper (Cu(2+)) concentrations are required for plants; however, at higher concentrations it can also cause multiple toxic effects. In the present study, lipid peroxidation, hydrogen peroxide levels as well as ascorbate peroxidase (APX: EC 1/11/1/11) and catalase (CAT: EC 1.11.1.6) activities were determined in Lycopersicum esculentum Mill. and Cucumis sativus L. seedlings after 7-day exposure to copper sulfate. In addition, DNA damage in these two crops was assessed by measuring micronucleus (MN) frequency and tail moments (TM) as determined by Comet assay. Inhibitory copper concentrations (EC(50): 30 and 5.5 ppm for L. esculentum and C. sativus, respectively) were determined according to dose-dependent root inhibition curves, and EC(50) and 2×EC(50) were applied. Malondialdehyde (MDA) and H(2)O(2) levels significantly increased in all groups studied. CAT activity increased in treatment groups of C. sativus. APX activity increased in L. esculentum seedlings due to 2×EC(50) treatment. Reductions in mitotic indices (MI) represented Cu(2+)dependent root growth inhibition in all treatment groups studied. According to TMs and MN frequencies, copper exposure induced significant DNA damage (p sativus roots. In conclusion, Cu(2+)induced oxidative damage, elevations in H(2)O(2) levels and alterations in APX and CAT activities, as well as significant DNA damage in nuclei of both study groups. To our knowledge, this is the first comparative and comprehensive study demonstrating the effects of copper on two different plant species at relevant cytotoxic concentrations at both biochemical and genotoxicity levels with multiple end points.

  5. Inbreeding and genetic diversity analysis in a hatchery release population and clones of Rhopilema esculentum based on microsatellite markers

    Science.gov (United States)

    Tian, Tao; Chen, Zaizhong; Wang, Mosang; Hu, Yulong; Wang, Weiji

    2017-05-01

    Ten microsatellite markers were used to analyze the levels of genetic diversity and inbreeding in a hatchery release population of Rhopilema esculentum Kishinouye (Scyphozoa: Rhizostomatidae). A total of 85 alleles were detected in 600 individuals. Within-population levels of observed ( H o) and expected ( H e) heterozygosity ranged from 0.152 to 0.839 (mean=0.464) and from 0.235 to 0.821 (mean=0.618), respectively. The polymorphism information content (PIC) of each marker ranged from 0.207 to 0.795 with an average of 0.580, indicating that the hatchery population maintained a high level of genetic diversity. Inbreeding levels were estimated in the hatchery population and the inbreeding coefficient was 0.203. This result revealed that a certain level of inbreeding occurred within the population. Meanwhile, we also determined genetic diversity at the clone level. Several polyps from the same scyphistomae were genotyped at the ten microsatellite loci and there was virtually no difference in their genotypes. Furthermore, we calculated the probabilities of exclusion. When both parents were known, the average exclusion probability of ten loci was 99.99%. Our data suggest that the ten microsatellite markers can not only be used to analyze the identity of individuals but they can also be applied to parentage identification. Our research provides a theoretical basis and technical support for genetic diversity detection and reasonable selection of R. esculentum hatchery populations. These findings support the use of releasing studies and conservation of R. esculentum germplasm resources.

  6. Inbreeding and genetic diversity analysis in a hatchery release population and clones of Rhopilema esculentum based on microsatellite markers

    Science.gov (United States)

    Tian, Tao; Chen, Zaizhong; Wang, Mosang; Hu, Yulong; Wang, Weiji

    2016-07-01

    Ten microsatellite markers were used to analyze the levels of genetic diversity and inbreeding in a hatchery release population of Rhopilema esculentum Kishinouye (Scyphozoa: Rhizostomatidae). A total of 85 alleles were detected in 600 individuals. Within-population levels of observed (H o) and expected (H e) heterozygosity ranged from 0.152 to 0.839 (mean=0.464) and from 0.235 to 0.821 (mean=0.618), respectively. The polymorphism information content (PIC) of each marker ranged from 0.207 to 0.795 with an average of 0.580, indicating that the hatchery population maintained a high level of genetic diversity. Inbreeding levels were estimated in the hatchery population and the inbreeding coefficient was 0.203. This result revealed that a certain level of inbreeding occurred within the population. Meanwhile, we also determined genetic diversity at the clone level. Several polyps from the same scyphistomae were genotyped at the ten microsatellite loci and there was virtually no difference in their genotypes. Furthermore, we calculated the probabilities of exclusion. When both parents were known, the average exclusion probability of ten loci was 99.99%. Our data suggest that the ten microsatellite markers can not only be used to analyze the identity of individuals but they can also be applied to parentage identification. Our research provides a theoretical basis and technical support for genetic diversity detection and reasonable selection of R. esculentum hatchery populations. These findings support the use of releasing studies and conservation of R. esculentum germplasm resources.

  7. Remote sensing and serological analysis of the resistance of tomato plants (Lycopersicon escylentum L.) to Tomato mosaic virus (ToMV)

    Science.gov (United States)

    Krezhova, Dora; Hristova, Dimitrina; Iliev, Ilko; Yanev, Tony

    Diseases caused by Tomato mosaic virus (ToMV) are among the most important factors lim-iting tomato production worldwide, as they can completely destroy the crop. ToMV occurs in most countries of the world, and causes disease epidemics in many crops. Systemic acquired resistance (SAR) is an inducible defence mechanism that plays a central role in disease re-sistance. SAR is induced by most pathogens that cause tissue necrosis. Spectral reflectance and chlorophyll fluorescence analysis were applied to establish injury of young tomato plants (Lycopersicon escylentum L.) infected with ToMV. Leaf spectral reflectance and chlorophyll fluorescence were registered by a portable Ocean Optics spectrometer USB 2000 in the visi-ble and near infrared spectral ranges (450-850 nm) at a spectral resolution of 1.5 nm. As a model system, tomato plants of cultivar Nuton resistant to ToMV were used. The plants were grown in a green house under controlled conditions. They were divided into six groups. The first group consisted of untreated (control) plants. At growth stage 4-6 expanded leaf, the second group was inoculated with ToMV. The other four groups were treated with following growth regulators: preparations Spermine, MEIA (beta-monomethyl ester of itaconic acid), (benzo(1,2,3)thiadiazole-7-carbothioic acid-S-methyl ester) and Phytoxin VS. On the next day, the tomato plants of these four groups were inoculated with ToMV. The viral concentrations in the plants were determined by the serological method Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). All analysis were performed on detached leaves from 20 uninfected and up to 20 leaves from infected plants on the 7th and 14th day after the inocu-lation. The differences between the reflectance spectra of virus-infected and uninfected leaves were analysed in the four most informative for green plants wavelength intervals: green (520-580 nm), red (640-680 nm), red edge (690-710 nm) and near infrared (720-760 nm

  8. Antihypertensive effect of long-term oral administration of jellyfish (Rhopilema esculentum) collagen peptides on renovascular hypertension.

    Science.gov (United States)

    Zhuang, Yongliang; Sun, Liping; Zhang, Yufeng; Liu, Gaoxiang

    2012-02-01

    Antihypertensive effect of long-term oral administration of jellyfish (Rhopilema esculentum) collagen peptides (JCP) on renovascular hypertension rats (RVHs) was evaluated. The systolic blood pressure and diastolic blood pressure of the RVHs were significantly reduced with administration of JCP (p oral treatment with high dose JCP (p > 0.05). Furthermore, effect of JCP on angiotensin II (Ang II) concentration of plasma had no significance (p > 0.05), but JCP significantly inhibited the Ang II concentration in RVHs' kidney (p < 0.05). The kidney should be the target site of JCP.

  9. Aplicação de coberturas proteicas e fenólicas em tomates (Lycopersicum esculentum)

    OpenAIRE

    Eliane Pereira Cipolatti; Larine Kupski; Meritaine da Rocha; Melissa dos Santos Oliveira; Jaqueline Garda Buffon; Eliana Badiale Furlong

    2012-01-01

    The aim of this study was to investigate the use of protein-phenolic based coating made from fermented rice bran on cherry tomatoes (Lycopersicum esculentum). Tests were performed with glycerol 3% (v/v), glycerol with protein-phenolic rice bran extract (5%), glycerol with protein-phenolic extract after 96 hours of fermentation (5%), and a control (without coating). The coated cherry tomatoes were kept at room temperature for 28 days. Mass loss, pH and acidity, total soluble solids, and carote...

  10. Dicty_cDB: AFF396 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone ...cTOD1C15, mRNA sequence. 92 2e-15 2 AI778006 |AI778006.1 EST258885 tomato susceptible, Cornell Lycopersicon ... |AW219042.1 EST301524 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA c...1.1 EST355887 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD11C15

  11. Dicty_cDB: VFC392 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 17896.1 EST296610 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD1C...15, mRNA sequence. 92 2e-15 2 AI778006 |AI778006.1 EST258885 tomato susceptible, Cornell Lycopersicon escule...9042.1 EST301524 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone c...T355887 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD11C15 5' end

  12. EVALUACIÓN DE LA TOLERANCIA AL CALOR A NIVEL DE PLANTA EN EL GERMOPLASMA DE TOMATE (Solanum L. sección Lycopersicon subsección Lycopersicon CONSERVADO Ex Situ

    Directory of Open Access Journals (Sweden)

    Marilyn Florido

    2009-01-01

    Full Text Available Este trabajo se desarrolló con el objetivo de evaluar la tolerancia al calor en una muestra representativa del germoplasma de tomate (Solanum L. sección Lycopersicon subsección Lycopersicon conservado ex situ en las colecciones cubanas e identificar las accesiones más tolerantes para su empleo en los programas de mejoramiento genético. Para ello se evaluó el porcentaje de fructificación en los períodos óptimo y estresante (primavera-verano del cultivo, para calcular los criterios de estrés siguientes: índice de susceptibilidad al estrés (ISE, valor de tolerancia al estrés (TOL, índice de tolerancia al estrés (ITE, fructificación geométrica del estrés (FGE e índice de tolerancia relativo (IT. Se clasificaron las 122 accesiones por su tolerancia al calor a nivel de planta en base al porcentaje de fructificación en siembras de primavera-verano y la relación entre los índices de tolerancia evaluados se efectuó mediante análisis Biplot. Se pudo comprobar que en el germoplasma evaluado existieron accesiones con altos porcentajes de fructificación en el período de primavera-verano, lo cual indica que en el germoplasma que se conserva ex situ en Cuba existen accesiones tolerantes al calor, que pueden ser explotadas en los programas de mejoramiento genético para obtener variedades e híbridos con buen comportamiento en estas condiciones.

  13. RELACION ENTRE LA TERMOESTABILIDAD DE LA MEMBRANA, LA VIABILIDAD CELULAR Y LOS CRITERIOS DE TOLERANCIA EN LA EVALUACIÓN DE LA TOLERANCIA AL CALOR EN TOMATE (Solanum L. sección Lycopersicon subsección Lycopersicon

    Directory of Open Access Journals (Sweden)

    Marilyn Florido Bacallao

    2011-01-01

    Full Text Available El presente trabajo se desarrolló con el objetivo deconocer si existe variabilidad en cuanto a tolerancia al calor enuna muestra representativa del germoplasma de tomate (Solanum L. sección Lycopersicon subsección Lycopersicon conservadoex situ en las colecciones cubanas e identificar las accesionesmás tolerantes para su empleo en los programas de mejoramiento genético. Para ello se calcularon los parámetros estadísticos:media, rango y coeficiente de variación de los caracterestermoestabilidad de la membrana celular (TMC y viabilidadcelular (VC, evaluados a nivel de tejido, así como de los caracteresy criterios de tolerancia: fructificación potencial (FP, fructificaciónen condiciones de estrés (FE, fructificación geométrica del estrés(FGE, tolerancia (TOL, índice de susceptibilidad al estrés (ISE,índice de tolerancia al estrés (ITE e índice de tolerancia relativo(ITR, evaluados a nivel de planta. Se evaluó, asimismo, la relaciónexistente entre estos, mediante estimaciones del índice de coincidencia de las mejores y peores accesiones, y la correlaciónexistente entre ellos. Se pudo comprobar que las evaluaciones anivel de tejido, especialmente la TMC, tuvo altos índices de coincidencia y correlación positiva con los criterios de toleranciaa nivel de planta, sobre todo con ITE o FGE, determinándose 18accesiones que coincidentemente se clasificaron como tolerantes al calor por todos los indicadores evaluados, las cuales seproponen como progenitores en los programas de mejoramientopara tolerancia al calor en el cultivo.

  14. Textural properties of mango cultivars during ripening.

    Science.gov (United States)

    Jha, Shyam Narayan; Jaiswal, Pranita; Narsaiah, Kairam; Kaur, Poonam Preet; Singh, Ashish Kumar; Kumar, Ramesh

    2013-12-01

    Firmness and toughness of fruit, peel and pulp of seven different mango cultivars were studied over a ripening period of ten days to investigate the effects of harvesting stages (early, mid and late) on fruit quality. Parameters were measured at equatorial region of fruits using TA-Hdi Texture Analyzer. The textural characteristics showed a rapid decline in their behaviour until mangoes got ripened and thereafter, the decline became almost constant indicating the completion of ripening. However, the rate of decline in textural properties was found to be cultivar specific. In general, the changes in textural attributes were found to be significantly influenced by ripening period and stage of harvesting, but firmness attributes (peel, fruit and pulp) of early harvested mangoes did not differ significantly from mid harvested mangoes, while peel, fruit and pulp firmness of late harvested mangoes were found to be significantly lower than early and mid harvested mangoes.

  15. Effect of various sugar solution concentrations on characteristics of dried candy tomato (Lycopersicum esculentum

    Directory of Open Access Journals (Sweden)

    EDWI MAHAJOENO

    2010-07-01

    Full Text Available Buntaran W, Astirin PA, Mahajoeno M. 2009. Effect of various sugar solution concentrations on characteristics of dried candy tomato (Lycopersicum esculentum. Nusantara Bioscience 2: 55-61. The aims of the research were to study the effects of sugar syrup concentration on dried candy tomato characteristics and to determine the proper sugar solution concentration that gives the best characteristics of dry candy tomatoes. The research used Randomized Block Design Method with four treatments and six times repetitions. The treatment that be used was immersing the tomato in sugar solution, with concentration of A (40%, B (50%, C (60%, and D (70% group in 18 hours. The variables measured were water content, ash, vitamin C and organoleptic tests include flavor, color, flavor and texture test. Data were analyzed using ANOVA test (Analysis of Variance followed by DMRT (Duncan Multiple Range Test. The result showed that sugar solution concentration had different effect on water content, ash content, vitamin C content, texture, and organoleptic test for colour, taste, and flavor of the dry candy tomato. The best characteristics of dry tomato candy was obtained on A (40% group, with water content of 24.20%, ash content of 0.62%, and vitamin C content of 31.15 mg/100 g. Standar quality of water content for dry fruit candy was maximal 25% (SII No.0718-2003 and maximal allowed ash content for food materials was 1.0% (SII 0272.90. Vitamin C content was not much decreased compared with ripe tomato i.e. 30-40 mg/100. Organoleptic tets result indicated that A (40% group get the highest score, i.e. 3,98 for taste, 3,89 for flavor, and 3,98 for colour.

  16. Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis

    Institute of Scientific and Technical Information of China (English)

    QIAO Hongjin; LIU Xiangquan; ZHANG Xijia; JIANG Haibin; WANG Jiying; ZHANG Limin

    2013-01-01

    Amplified fragment length polymorphisms (AFLP) markers were developed to assess the genetic variation of populations and clones of Rhopilema esculentum Kishinouye (Scyphozoa,Rhizostomatidae).One hundred and seventy-nine loci from 56 individuals of two hatchery populations and two wild populations were genotyped with five primer combinations.The polymorphic ratio,Shannon's diversity index and average heterozygosity were 70.3%,0.346 and 0.228 for the white hatchery population,74.3%,0.313,and 0.201 for the red hatchery population,79.3%,0.349,and 0.224 for the Jiangsu wild population,and 74.9%,0.328 and 0.210 for the Penglai wild population,respectively.Thus,all populations had a relatively high level of genetic diversity.A specific band was identified that could separate the white from the red hatchery population.There was 84.85% genetic differentiation within populations.Individual cluster analysis using unweighted pair-group method with arithmetic mean (UPGMA) suggested that hatchery populations and wild populations could be divided.For the hatchery populations,the white and red populations clustered separately; however,for the wild populations,Penglai and Jiangsu populations clustered together.The genetic diversity at the clone level was also determined.Our data suggest that there are relatively high genetic diversities within populations but low genetic differentiation between populations,which may be related to the long-term use of germplasm resources from Jiangsu Province for artificial seeding and releasing.These findings will benefit the artificial seeding and conservation of the germplasm resources.

  17. Physical, chemical and biological characteristics of space flown tomato (Lycopersicum esculentum) seeds

    Science.gov (United States)

    Esyanti, Rizkita R.; Dwivany, Fenny M.; Almeida, Maria; Swandjaja, Leonita

    2016-11-01

    Several research showed that space flown treated seeds had a different characteristic with that of ground treated seed, which eventually produced a different characteristic of growth and productivity. Research was conducted to study the physical, chemical and biological properties, such as the rate of germination and the growth of tomato (Lycopersicum esculentum) space flown seeds compared with that of control one. Observations of physical properties using a SEM showed that there were pores on the surface of some tomato space flown seeds. Observations using a stereo and inverted microscope showed that the coat layer of space flown seeds was thinner than control seeds. The total mineral content in the control seeds (22.88%) was averagely higher than space flown seeds (18.66%), but the average carbohydrate content in control seed was lower (15.2 ± 2.79%) than the space flown seeds (9.02 ± 1.87%). The level of auxin (IAA) of control seeds (142 ± 6.88 ppm) was averagely lower than the space flown seeds (414 ± 78.84 ppm), whereas the level of cytokinins (zeatin) for the control seeds (381 ± 68.86 ppm) was higher than the space flown seeds (68 ± 9.53 ppm), and the level of gibberellin (GA3) for the control seeds (335 ± 10.7 ppm) was higher than the space flown seeds (184 ± 7.4 ppm). The results of this study showed that the physical and chemical properties of tomato space flown seeds were generally different compare with that to control seeds, so that it might also be resulted in different germination and growth characteristic. The germination test showed that space flown seeds had lower germination rate compare to control. The growth pattern indicated that planted space flown seeds generally grew better than control. However, those data were more homogenous in control seeds compare to that in space flown tomato seeds.

  18. Effects of methyl jasmonate on accumulation of flavonoids in seedlings of common buckwheat (Fagopyrum esculentum Moench).

    Science.gov (United States)

    Horbowicz, M; Wiczkowski, W; Koczkodaj, Danuta; Saniewski, M

    2011-09-01

    The jasmonates, which include jasmonic acid and its methyl ester (MJ), play a central role in regulating the biosynthesis of many secondary metabolites, including flavonoids, and also are signaling molecules in environmental stresses. Synthesis of anthocyanins pigments is a final part of flavonoids pathway route. Accumulation of the pigments in young seedlings is stimulated by various environmental stresses, such as high-intensity light, wounding, pathogen attack, drought, sugar and nutrient deficiency. The anthocyanins take part in defense system against excess of light and UV-B light, and therefore it is probably main reason why young plant tissues accumulate enlarged levels of the pigments. The effects of exogenously applied MJ on level of anthocyanins, glycosides of apigenin, luteolin, quercetin and proanthocyanidins in seedlings of common buckwheat (Fagopyrum esculentum Moench) were studied. MJ decreased contents of all the found cyanidin glycosides and its aglycone in hypocotyls of buckwheat seedlings. However contents of particular anthocyanins in cotyledons of buckwheat seedlings treated with the plant hormone were not significantly different from the control. Applied doses of MJ did not affect levels of quercetin, apigenin and luteolin glycosides in the analyzed parts of buckwheat seedlings: cotyledons and hypocotyls. On the other hand, treatment of buckwheat seedlings with MJ clearly stimulated of proanthocyanidins biosynthesis in hypocotyls. We suggest that methyl jasmonate induces in hypocotyls of buckwheat seedlings the leucocyanidin reductase or anthocyanidin reductase, possible enzymes in proanthocyanidins synthesis, and/or inhibits anthocyanidin synthase, which transforms leucocyanidin into cyanidin. According to our knowledge this is the first report regarding the effect of methyl jasmonate on enhancing the accumulation of proanthocyanidins in cultivated plants.

  19. Relative competitiveness of soybean cultivars with barnyardgrass

    Directory of Open Access Journals (Sweden)

    Marlon Ouriques Bastiani

    Full Text Available ABSTRACT The aim of this work was to evaluate the competitiveness between soybean cultivars and barnyardgrass, based on morphological and physiological characteristics of species. The experiments were conducted in completely randomized experimental design, with 4 replications. In the first study, for both soybean and barnyardgrass, it was determined the population of plants in which shoot dry matter became constant and independent of the population (16 plants∙pot−1 or 400 plants∙m−2. In the second study, 2 experiments were conducted to evaluate the competitiveness of BMX Apolo RR and BMX Potência RR soybean cultivars with barnyardgrass plants, both carried out in replacement series under different proportions of plants∙pot−1 (100:0; 75:25; 50:50; 25:75 and 0:100 between the crop and the weed. The analysis of the species competitiveness was determined through diagrams applied to replacement series experiments and use of relative competitiveness indexes. At 44 days after the emergence of species, the physiological and morphological parameters of the crop and the weed were evaluated. The BMX Apolo RR and BMX Potência RR soybean cultivars show similar competitiveness when competing with the barnyardgrass; therefore, the ability of one species to interfere on another is equivalent. For plant height, barnyardgrass displays higher competitiveness compared to BMX Apolo RR, with early cycle and short height. The intraspecific competition is more important to barnyardgrass than interspecific competition with soybean cultivars, resulting in negative effects on the morphological and physiological characteristics of species.

  20. RB975952 – Early maturing sugarcane cultivar

    Directory of Open Access Journals (Sweden)

    Monalisa Sampaio Carneiro

    2015-08-01

    Full Text Available RB975952 is an early maturing sugarcane cultivar released for the South-Central region of Brazil. It should be harvested between April and May, and it is recommended for planting in environments with medium to high production potential. RB975952 has high resistance levels to the main diseases of the crop, it also has a good shoot development after mechanical harvesting, and high sucrose yields.

  1. Evaluation of garlic cultivars for polyphenolic content and antioxidant properties.

    Directory of Open Access Journals (Sweden)

    Shuxia Chen

    Full Text Available Two phenolic compound parameters (total phenolic and flavonoid contents and 5 antioxidant parameters (DPPH [2, 2-diphenyl-1-picrylhydrazyl] radical scavenging activity, HRSC (hydroxyl radical scavenging capacity, FRAP (ferric ion reducing antioxidant power, CUPRAC (cupric ion reducing antioxidant capacity, and MCA (metal chelating activity were measured in bulbs and bolts of 43 garlic cultivars. The bulbs of cultivar '74-x' had the highest phenolic content (total phenolic, flavonoids and the strongest antioxidant capacity (DPPH, FRAP, and CUPRAC, followed by bulbs of cultivar 'Hanzhong purple'; the bulbs of cultivar 'Gailiang' had the lowest phenolic content and antioxidant capacity (FRAP, CUPRAC, MCA. The bolts of 'Hanzhong purple' also had higher phenolic content. Principal components analysis (PCA separated the cultivars into 3 groups according to phenolic and flavonoid contents and strength of antioxidant activity. The first group had higher HRSC, FRAP, and flavonoid content; the second group had higher total phenolic content and MCA; some cultivars in the third group had higher HRSC and FRAP. All 8 test garlic bulb extracts successfully prevented Human Vascular Endothelial Cell death and significantly prevented reactive-oxygen species (ROS formation in oxidative stress model, in which cultivar '74-x' had highest protection capability, following by cultivar 'Hanzhong purple', and the bulbs of cultivar 'No. 105 from Korea' had the lower protection capability against cell death and ROS formation. The protection capability in vivo of these garlic cultivars was consistent with their phenolic content and antioxidant capacity.

  2. Evaluation of garlic cultivars for polyphenolic content and antioxidant properties.

    Science.gov (United States)

    Chen, Shuxia; Shen, Xiaoqing; Cheng, Siqiong; Li, Panpan; Du, Junna; Chang, Yanxia; Meng, Huanwen

    2013-01-01

    Two phenolic compound parameters (total phenolic and flavonoid contents) and 5 antioxidant parameters (DPPH [2, 2-diphenyl-1-picrylhydrazyl] radical scavenging activity, HRSC (hydroxyl radical scavenging capacity), FRAP (ferric ion reducing antioxidant power), CUPRAC (cupric ion reducing antioxidant capacity), and MCA (metal chelating activity) were measured in bulbs and bolts of 43 garlic cultivars. The bulbs of cultivar '74-x' had the highest phenolic content (total phenolic, flavonoids) and the strongest antioxidant capacity (DPPH, FRAP, and CUPRAC), followed by bulbs of cultivar 'Hanzhong purple'; the bulbs of cultivar 'Gailiang' had the lowest phenolic content and antioxidant capacity (FRAP, CUPRAC, MCA). The bolts of 'Hanzhong purple' also had higher phenolic content. Principal components analysis (PCA) separated the cultivars into 3 groups according to phenolic and flavonoid contents and strength of antioxidant activity. The first group had higher HRSC, FRAP, and flavonoid content; the second group had higher total phenolic content and MCA; some cultivars in the third group had higher HRSC and FRAP. All 8 test garlic bulb extracts successfully prevented Human Vascular Endothelial Cell death and significantly prevented reactive-oxygen species (ROS) formation in oxidative stress model, in which cultivar '74-x' had highest protection capability, following by cultivar 'Hanzhong purple', and the bulbs of cultivar 'No. 105 from Korea' had the lower protection capability against cell death and ROS formation. The protection capability in vivo of these garlic cultivars was consistent with their phenolic content and antioxidant capacity.

  3. Effect of Heat Shock Treatment and Aloe Vera Coating to Chilling Injury Symptom in Tomato (Lycopersicon asculantum Mill.

    Directory of Open Access Journals (Sweden)

    Sutrisno

    2012-04-01

    Full Text Available This research was undertaken to determine the effect of length in heat shock and edible coating as pre-storage treatment to Chilling Injury (CI symptom reflected by ion leakage induced and quality properties in tomato (Lycopersicon asculantum Mill.. Heat Shock Treatment (HST was conducted at three different levels of length, which were, 20; 40 and 60 min. Edible coating was conducted using aloe vera gel. The result showed that HST and Aloe Vera Coating (AVC were more effective to reduce CI symptom at lower chilling storage. Prolong exposure to heated water may delay climacteric peak. The length of heat shock, AVC treatment and low temperature storage significantly affected the tomato quality parameter but not significantly different for each treatment except weight loss. HST for 20 min at ambient temperature was significantly different to other treatment.

  4. ASPECTOS MORFOANATÔMICOS DE FRUTOS DE TOMATEIRO CULTIVAR ÂNGELA GIGANTE, SUBMETIDOS A TRATAMENTOS COM REGULADORES VEGETAIS

    Directory of Open Access Journals (Sweden)

    MARIA BERNARDETE GONÇALVES MARTINS

    1997-01-01

    Full Text Available O presente trabalho teve como finalidade obter dados morfológicos de frutos de tomateiro, Lycopersicon esculentum Mill. `Ângela Gigante', submetidos à ação de reguladores vegetais, em dois ensaios que ocorreram em épocas distintas, em casa de vegetação. As mudas foram selecionadas e transplantadas para vasos com capacidade de 12 L de terra, contendo uma mistura de solo argiloso, areia, matéria orgânica e uma adubação mineral complementar de N, P, K. No segundo ensaio, após o transplante das mudas, além da adubação mineral complementar de N, P, K, efetuaram-se adubações adicionais (fertirrigação. Em ambos os ensaios, quando as plantas atingiram quatro folhas definitivas, realizaram-se as pulverizações com giberelina, GA3 50 mg/L; ácido naftalenacético, NAA 100 mg/L; cloreto (2-cloroetil trimetilamônio, CCC 1.500 mg/L e ácido succínico -2,2 dimetil-hidrazida, SADH 3.000 mg/L. Em relação aos estudos anatômicos, observou-se que os tratamentos com retardadores vegetais (CCC e SADH produziram frutos firmes, com formato tipo barril e ombros salientes; entretanto, em seção transversal, notou-se perda de viscosidade e atrofia de sementes, principalmente nos frutos de plantas tratadas com SADH. Os tratamentos com NAA e GA3 causaram eventual formação de frutos geminados. O tratamento com GA3 apresentou o parênquima do pericarpo com grãos de amido em processo de fragmentação, provavelmente em virtude de o GA3 acelerar a atividade da amilase, afetando o processo de maturação dos frutos e transformando o amido em açúcares. Notaram-se no mesocarpo células com grande quantidade de cristais de oxalato de cálcio sob a forma de areia cristalina. Do tratamento com CCC resultaram frutos suculentos com células da placenta degeneradas, deixando livre grande quantidade de mucilagem. O pericarpo apresentou grande quantidade de grãos de amido composto em toda a extensão, provavelmente por haver um atraso no processo de

  5. Cadmium accumulation in different rice cultivars and screening for pollution-safe cultivars of rice

    Energy Technology Data Exchange (ETDEWEB)

    Yu Hui [State Key Laboratory for Biocontrol, also School of Life Sciences, Sun Yat-sen University, Guangzhou 510275 (China); Wang Junli [State Key Laboratory for Biocontrol, also School of Life Sciences, Sun Yat-sen University, Guangzhou 510275 (China); Fang Wei [Biology Department, Long Island University, Brooklyn, NY 11201 (United States); Yuan Jiangang [State Key Laboratory for Biocontrol, also School of Life Sciences, Sun Yat-sen University, Guangzhou 510275 (China); Yang Zhongyi [State Key Laboratory for Biocontrol, also School of Life Sciences, Sun Yat-sen University, Guangzhou 510275 (China)]. E-mail: adsyzy@mail.sysu.edu.cn

    2006-11-01

    Large areas of contaminated land are being used for agricultural production in some countries due to the high demand for food. To minimize the influx of pollutants to the human food chain through consumption of agricultural products, we propose the concept of pollution-safe cultivars (PSCs), i.e. cultivars whose edible parts accumulate a specific pollutant at a level low enough for safe consumption, even when grown in contaminated soil. We tested the feasibility of the PSC concept by growing 43 cultivars of paddy rice (Oryza sativa L., including 20 normal and 23 hybrid cultivars) under a high (75.69-77.55 mg kg{sup -1}) and a low (1.75-1.85 mg kg{sup -1}) cadmium (Cd) exposure. These pot experiments took place in the spring and summer of 2004. At the low level of Cd exposure, 30 out of the 43 tested cultivars were found to be Cd-PSCs. Grain Cd concentrations were highly correlated (p < 0.01) between the two experiments, suggesting that Cd accumulation in rice grain is genotype-dependent and that the selection of PSCs is possible, at least at a certain level of soil contamination. No Cd-PSCs were found under the high level of Cd exposure. Yield was enhanced in some cultivars and depressed in others in response to elevated soil Cd, indicating that farmers cannot rely on yield depression as an indicator of toxicity of the grains. It is therefore important and feasible to screen for PSCs and to establish PSC breeding programs to effectively and efficiently reduce the risk of human exposure to soil pollutants, such as Cd, through crop consumption.

  6. Screening of extraction methods for glycoproteins from jellyfish ( Rhopilema esculentum) oral-arms by high performance liquid chromatography

    Science.gov (United States)

    Ren, Guoyan; Li, Bafang; Zhao, Xue; Zhuang, Yongliang; Yan, Mingyan; Hou, Hu; Zhang, Xiukun; Chen, Li

    2009-03-01

    In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish ( Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear ( r = 0.9984, y = 4.5895 x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish ( R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.

  7. Screening of Extraction Methods for Glycoproteins from Jellyfish (Rhopilema esculentum) Oral-Arms by High Performance Liquid Chromatography

    Institute of Scientific and Technical Information of China (English)

    REN Guoyan; LI Bafang; ZHAO Xue; ZHUANG Yongliang; YAN Mingyan; HOU Hu; ZHANG Xiukun; CHEN Li

    2009-01-01

    In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotcin. The results showed that the calibration curves for peak area plotted against con-centration for TGP were linear (r=0.9984, y=4.5895x+47.601) over concentrations ranging from 50 to 400mgL-1. The mean extrac-tion recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms bv four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water ex-traction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophtlized extract was dissolved in Milli-Q water and ana-lyzed directly on a short TSK-GEL G4000PWXL (7.8mm×300mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.Kay words HPLC; glycoprotein; jellyfish; extraction method

  8. Characterization of oats (Avena sativa L.) cultivars using machine vision.

    Science.gov (United States)

    Sumathi, S; Balamurugan, P

    2013-10-15

    Machine vision or image analysis is an important tool in the study of morphology of any materials. This technique has been used successfully to differentiate the eleven oats cultivars based on morphological characters. The geometry of seeds was measured through image analyzer and the variation was observed and recorded. From the recorded data, the cluster analysis was carried out and it revealed that the cultivars could be grouped into two main clusters based on similarity in the measured parameters. Cultivar Sabzar, UPO 212, OL 9 and OL 88 formed one main cluster. The another main cluster includes cv. Kent, OS 6, UPO 94, HFO 114, OS 7, HJ 8 and JHO 822 with many sub clusters. Among the cultivars HJ 8 and JHO 822 has more similarity in all measured parameters than other cultivars. Thus morphological characterization through seed image analysis was found useful to discriminate the cultivars.

  9. Dicty_cDB: VFG581 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 6610 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDN...A clone cTOD1C15, mRNA sequence. 92 3e-15 2 AI778006 |AI778006.1 EST258885 tomato susceptible, Cornell Lycop...524 tomato root during/after fruit set, Cornell University Lycopersicon esculentu...o flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cT

  10. Rooting of stem segments from fig tree cultivars

    OpenAIRE

    Rayane Barcelos Bisi; Guilherme Locatelli; Caio Morais de Alcântara Barbosa; Rafael Pio; Rodrigo Vieira Balbi

    2016-01-01

    ABSTRACT. Although Brazil is the largest fig (Ficus carica L.) producer in the Southern Hemisphere, it mainly uses only one cultivar, 'Roxo de Valinhos'. In addition, propagation is almost entirely through hardwood cuttings. Therefore, the aim of this study was to establish a propagation method that provides more successful rooting of stem segments of fig cultivars for the purpose of expanding the genetic base of the fig tree. The cultivars used were 'Brunswick', 'Calabacita', 'Negro de Bursa...

  11. Screening of Carnation Cultivars for Resistance to Meloidogyne incognita

    OpenAIRE

    Cho, M. R.; Kim, J. Y.; Song, C.; Ko, J.Y.; Na, S. Y.; Yiem, M. S.

    1996-01-01

    A total of 33 carnation cultivars cultured in Korea were screened for resistance to the southern root-knot nematode, Meloidogyne incognita. Carnations were tested by either inoculating with 5,000 eggs or by transplanting into a mixture of bedding medium and soil infested with an average of 435 second-stage juveniles/300 cm³ soil. Cultivars, Desio, Castelaro, Kappa, Rara, Izu Pink, Target, and Antalia were highly resistant to M. incognita. Twelve cultivars were moderately resistant, and the re...

  12. Cultivar identification in T. aestivum using highly polymorphic RFLP probes.

    Science.gov (United States)

    Vaccino, P; Accerbi, M; Corbellini, M

    1993-08-01

    Two probes, specific for HMW-glutenins and γ-gliadins have been used to identify 50 common wheat Italian cultivars, most of which are closely related, and four common wheat cultivars originating outside Italy. The probes revealed complex polymorphic patterns; three probe/enzyme combinations had the necessary sensitivity for the identification of all 54 cultivars. As already shown for potato and barley, the use of four-cutter restriction enzymes and polyacrylamide gels proved particularly useful for detecting polymorphism.

  13. Photosynthetic Diurnal Variation of Soybean Cultivars with High Photosynthetic Efficiency

    Institute of Scientific and Technical Information of China (English)

    MAN Wei-qun; DU Wei-guang; ZHANG Gui-ru; LUAN Xiao-yan; GE Qiao-ying; HAO Nai-bin; CHEN Yi

    2002-01-01

    The photosynthetic characters were investigated among soybean cultivars with high photosynthetic efficiency and high yield. The results indicated that: 1) There were significant differences in photosynthetic rate (Ph) and dark respiration rate (DR) under saturation light intensity and appropriate temperature.2) There were a little difference in light compensation point among them. Photo flux density (PFD) were mong the cultivars. Diurnal variation of Pn was shown a curve with two peaks. 4) The cultivars with high photosynthetic efficiency were subjected less to photoinhibition than that with high yield. Critical temperatures of photoinhibition in high photosynthetic efficiency cultivars were higher than that of high yield.

  14. Rational regional distribution of sugarcane cultivars in China.

    Science.gov (United States)

    Luo, Jun; Pan, Yong-Bao; Xu, Liping; Grisham, Michael Paul; Zhang, Hua; Que, Youxiong

    2015-10-26

    Knowing yield potential and yield stability of sugarcane cultivars is of significance in guiding sugarcane breeding and rationalising regional distribution of sugarcane cultivars. In the present study, a heritability-adjusted genotype main effect plus genotype × environment (HA-GGE) biplot program was used to analyze the cane and sucrose yields of 44 newly released sugarcane cultivars at eight pilot test sites. The cane and sucrose yields of nine cultivars were higher than those of the control cultivar ROC22. From the perspective of cane yield, cultivars FN 40 and YZ 06-407 were well adapted to a wider range of conditions and produced relatively high cane yields in several pilot sites. From the perspective of sucrose yield, cultivars LC 03-1137, FN 38, FN 41, MT 01-77 and LC 05-136 were well adapted to a wide range of conditions and produced relatively high sucrose yields. Based on these results, three high yielding and widely adapted cultivars, namely, FN 39, LC 05-136, and YZ 05-51 were recommended for production in three major Chinese sugarcane planting areas. The results will provide a theoretical basis for recommending the effective use and rational regional distribution of sugarcane cultivars in China.

  15. Consumer evaluation of scab-resistant apple cultivars in Sweden

    Directory of Open Access Journals (Sweden)

    Å. JÖNSSON

    2008-12-01

    Full Text Available The feasibility of using untrained consumers for sensory evaluation was investigated on Swedish-grown scab-resistant apple cultivars in 2002 (11 cultivars and 2003 (15 cultivars. The consumers evaluated five cultivars each, including the control cultivar ‘Aroma’. Each cultivar was scored for appearance, texture, juiciness, taste and overall impression by a minimum of 100 consumers in 2002 and by 200 in 2003. All quality attributes were positively correlated when calculations were performed across all consumer scores, with overall impression and taste having the strongest association. ‘Rubinola’ and K:1160 emerged as the best liked cultivars overall. Cultivar means were analysed for relationships with some previously scored or measured variables and with consumer profile data. Positive correlations with overall impression were obtained for amount of red colour in 2002, and for sugar levels in 2003. Among consumer profile variables, age had the largest influence on consumer scores, with some indications that older consumers preferred (or at least tolerated soft-fleshed cultivars better than did younger consumers. Minimum number of consumers needed to obtain reliable results was determined by computer simulations indicating that the error rate became stabilized already with 50 consumers.;

  16. Zinc and copper uptake by plants under two transpiration rates. Part II. Buckwheat (Fagopyrum esculentum L.).

    Science.gov (United States)

    Tani, F H; Barrington, S

    2005-12-01

    To evaluate the environmental risks of irrigating crops with treated wastewater, a study was undertaken to quantify heavy metal uptake by 4-week old buckwheat (Fagopyrum esculentum L.) plants during 18 days of irrigation with 8 different Cu and Zn solutions under two transpiration rates (TR). At 4 weeks, potted buckwheat plants were transferred into one of the two growth chambers, offering either a high or low vapour pressure deficit (VDP) for, respectively, a high or low TR. Triplicate pots received one of the 8 irrigation treatments containing one of two Zn levels (0 and 25 mg/L) combined with one of four Cu levels (0, 5, 10 and 15 mg/L). Daily TR were measured by weighing the evapo-transpired water lost from the planted pot, less was the evaporation loss measured from triplicate non-planted pots. After 0, 6, 12 and 18 days of treatment, the stems and leaves of three randomly selected plants were harvested and after 18 days, the roots were harvested to determine Cu and Zn uptake. The treatments did not affect TR in terms of dry plant mass, indicating the absence of toxic effects. Irrigating with Zn, without Cu, increased dry biomass production, whereas the lowest biomass occurred with 15 and 30 mg/L of Cu with and without 25 mg/L of Zn, respectively, because higher applications of heavy metal significantly reduced soil pH. Plant Cu and Zn uptake increased with TR. With higher levels of Cu, Zn uptake by buckwheat was significantly reduced, while Zn had a slight but non-significant impact on Cu uptake. Previously and in a study exposing wheat plants to the same conditions, Cu significantly increased Zn uptake, while Zn had a slight but insignificant negative effect on Cu uptake. The buckwheat roots contained the greatest levels of Cu and Zn, indicating their role in moderating heavy metal uptake. Also, both Cu and Zn had a synergetic effect on each other in terms of root levels, and a similar observation was made in the earlier similar experiment using wheat plants

  17. CULTIVAR RELEASE-BRS 217 Flora: Early-maturing soybean cultivar

    Directory of Open Access Journals (Sweden)

    Plínio Itamar de Mello de Souza

    2008-01-01

    Full Text Available The soybean (Glycine max L. Merr. cultivar BRS 217 Flora was developed by Embrapa and released forproduction in the states of Goiás, Minas Gerais, Bahia, Mato Grosso and the Distrito Federal, Brazil. It is resistant to stemcanker, frog-eye leaf spot, bacterial pustule, and partially resistant to powdery mildew.

  18. UEL 7: nova cultivar de Dendrobium UEL 7: a new Dendrobium cultivar

    Directory of Open Access Journals (Sweden)

    Ricardo Tadeu de Faria

    2011-09-01

    Full Text Available UEL 7 é uma cultivar de Dendrobium nobile desenvolvida no Departamento de Agronomia da Universidade Estadual de Londrina resultante do cruzamento das plantas matrizes D3 x D8 e selecionada pela coloração das flores amarelas e com largura e comprimento semelhantes (7,0 cm, formando praticamente um círculo. As plantas foram polinizadas artificialmente, e as sementes semeadas em meio de cultura in vitro. A cultivar apresenta flores de coloração amarela, labelo com o centro marrom escuro e o pseudobulbo apresenta em mé dia 11 flores. As flores têm durabilidade de aproximadamente 30 dias.UEL 7 is a Dendrobium nobile's cultivar developed at The Department of Agronomy of Universidade Estadual de Londrina and is the result of the crossing between D3 x D8 and selected for its yellow flowers and format with similar width and length (7.0 cm, almost forming a circle. The plants were pollinated artificially, and the seeds sowed in growth media in vitro. The cultivar presents flowers of yellow coloration, labelo with the center dark brown color and the pseudobulb presents 11 flowers on average. The flowers durability is about 30 days.

  19. CULTIVAR RELEASE - FAEM Carlasul: new white oat cultivar with high grain yield

    Directory of Open Access Journals (Sweden)

    Antônio Costa de Oliveira

    2012-01-01

    Full Text Available The white oat cultivar FAEM Carlasul was developed at the Plant Genomics and Breeding Center, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, as a result of the cross between UFRGS 10 and 90SAT-28 (Coronado2/Cortez3/Pendek/ME 1563. It is characterized by high yield and grain quality.

  20. Efeito do uso de diferentes compostos na produção de tomate (Lycopersicum esculentum Mill em cultivo orgânico

    Directory of Open Access Journals (Sweden)

    M. S. Teodoro

    2015-12-01

    Full Text Available O objetivo do presente trabalho foi avaliar, em condições de casa de vegetação, a influência de cinco compostos orgânicos na adubação de plantio e de cobertura de tomate híbrido do tipo Caqui. O delineamento experimental adotado foi o de blocos casualizados, com quatro repetições. Foram avaliados cinco tratamentos, contendo biomassa vegetal provenientes do banco de leguminosas da Embrapa Meio-Norte. As leguminosas utilizadas na confecção dos compostos foram o guandu Cultivar Fava Larga (Cajanus cajan, a Crotalária juncea (Crotalaria juncea L., a Leucena (Leucaena leucocephala, a Gliricídia (Gliricidia sepium e a Mucuna preta (Mucuna aterrima. A gramínea utilizada foi o capim elefante (Pennisetum purpureum. Os frutos do tomate começaram a ser colhidos no início do amadurecimento, por volta de 70 a 120 dias após o transplantio. Avaliaram-se a produção total de frutos (PTF, produção de frutos comerciais (PFC, número total de frutos (NTF, número de frutos comerciais (NFC, peso médio de frutos comerciais (PMFC e diâmetro médio de frutos (DMF. Foram registradas ocorrências de pragas nas condições experimentais, detectando-se a presença de nematóides (Meloydogines spp e cochonilha (Maconellicoccus hirsutus. Não houve diferença significativa entre os tratamentos estudados, entretanto, o tratamento 2 foi o que se destacou quanto as variáveis analisadas, principalmente para PTF (54,5 t/ha e PFC (49,6 t/ha. Concluiu-se que o uso de compostos orgânicos com palhadas de leguminosas e gramíneas é uma alternativa técnica e economicamente viável, podendo reduzir o uso integral do esterco bovino na adubação de plantio e de cobertura do tomate produzido no sistema orgânico.Effect of the use of different compounds in the production of tomato (Lycopersicum esculentum Mill in organic farmingAbstract: - The purpose of this study was to evaluate, in greenhouse conditions, the influence of five organic compounds on fertilizing