Institutional Memory and the US Air Force

Science.gov (United States)

2016-01-01

38 | Air & Space Power Journal Institutional Memory and the US Air Force Lt Col Daniel J. Brown, USAF Disclaimer: The views and opinions expressed...or implied in the Journal are those of the authors and should not be construed as carrying the official sanction of the Department of Defense, Air...national defense. After each ad- vance is tested in combat, a new round of intellectual sparring commences regarding Summer 2016 | 39 Institutional Memory

Air Force Academy Aeronautics Digest, Spring/Summer 1980

Science.gov (United States)

1980-10-01

Forc- Academy, CO 80840 II CONTROLLING OFFICE NAME AND ADDRESS I 3-WOMBEIR OF PGI I1. MONITORING AGENCY NAiE A AOFlighdtipIt Ican.Co pu10olsin O nc*) I...the 1700’s. Over the years Pitot tubes have been made extremely small so as not to disturb the flow field with the intruding probe. Additionally, the...as experience with automited data acqtIsition systems increases. Some very general method,, of calibra- *Lt Col, USAF, Tenure Associate Professor of

USAF Weapon System Evaluation Program

National Research Council Canada - National Science Library

1999-01-01

During this task period, Schafer Corporation provided engineering services and analysis to the USAF at Eglin AFB, Florida in direct support of the USAF Air-to-Surface Weapon System Evaluation Program (WSEP...

An Economic Analysis of the Truth in Negotiations Act

Science.gov (United States)

2016-01-27

Associate Professor 1st Lt Crystal Champion, USAF Capt Meredith Ellen, USAF Capt Jenny Walk, USAF Graduate School of Business & Public Policy...Professor 1st Lt Crystal Champion, USAF Capt Meredith Ellen, USAF Capt Jenny Walk, USAF Graduate School of Business & Public Policy Naval...appropriate cases, an adjustable price. Fixed-price contracts providing for an adjustable price may include a ceiling price, a target price

Air & Space Power Journal. Volume 22, Number 3, Fall 2008

Science.gov (United States)

2008-09-01

5 Lt Gen Stephen G . Wood, USAF Maj Christopher A . Johnson, DM , USAF...Lt Gen Stephen G. Wood, USAF MAj ChriStopher A. johnSon, dM , USAF Today nearly 28,000 american warriors stand shoulder to shoulder with our...their book with a crisp sum- mary of military strategy and war between the eigh- teenth and late twentieth centuries to show that al- though the practices

The American Defense of Long Island 1776: Destined for Failure?

Science.gov (United States)

2008-01-01

Smallwood’s Md. Cont., Col. William Smallwood Haslet’s Del. Cont., Col. John Haslet Pa. State Rifle Rgt., Col. Samuel Miles Pa. State Bn. ofMusketry, Col...Foot, Maj. Norman Lamont ofLamont 16th Light Dragoons, Lt. Col. William Harcourt 17th Light Dragoons, Lt. Col. Samuel Birch Corps de Reserve: Lt. Gen

Expression of cysLT1 and cysLT2 Receptor in Chronic Hyperplastic Eosinophilic Sinusitis

International Nuclear Information System (INIS)

Ouyang, Yuhui; Kamijo, Atsushi; Murata, Shin-ichi; Okamoto, Atsushi; Endo, Shuichiro; Katoh, Ryohei; Masuyama, Keisuke

2009-01-01

Elevated production of cysteinyl leukotrienes (cysLTs) from sinus tissues and abundant sinus eosinophils are characteristic features of chronic hyperplastic eosinophilic sinusitis (CHS). CysLTs exert their action through G-protein-coupled receptors named cysLTs receptor type I (cysLT1R) and type II (cysLT2R). These expressions of cysLT receptors in the sinus mucosa have yet to be clarified and the relationship between eosinophilia and the expression of these receptors remains obscure. We compared the expressions of cysLT1R and cysLT2R in the sinus mucosa in patients with CHS, non-eosinophilic chronic sinusitis (NECS), and control sinus tissues; and analyzed the correlation between the expression of CysLTRs and the presence of sinus eosinophils by immunohistochemistry and real-time PCR. A significantly higher percentage of eosinophils expressing cysLT2R protein was observed in patients with CHS compared with NECS and controls. In addition, cysLT2R mRNA expression in CHS was significantly higher than in NECS and controls. Furthermore, a positive correlation was observed between cysLT2R mRNA expression and the number of infiltrated eosinophils. In contrast, the cysLT1R mRNA expression did not differ significantly among these groups. The effect of cysLTs on sinus eosinophils may be mediated through the cysLT2R in patients with CHS. These results may suggest the therapeutic benefit of cysLT2R antagonists in CHS

USAF Institute for National Security Studies 1998 Research Results Conference

National Research Council Canada - National Science Library

1998-01-01

The USAF Institute for National Security Studies (INSS), in cooperation with HQ USAF Nuclear and Counterproliferation Directorate, sponsored its 6th annual Research Results Conference on 19 - 20 November 1998...

Combination L-T3 and L-T4 therapy for hypothyroidism.

Science.gov (United States)

Wartofsky, Leonard

2013-10-01

Because of the longstanding controversy regarding whether hypothyroid patients can be optimally replaced by treatment with levothyroxine (L-T4) alone, numerous studies have addressed potential benefits of combined therapy of triiodothyronine (T3) with L-T4. Results of these studies have failed to support a potential benefit of combined therapy. A strong argument for the addition of L-T3 to L-T4 monotherapy has been lacking until recent genetic studies indicated a rationale for such therapy among a small fraction of the hypothyroid patient population. Interest in this issue has focused on the importance of the deiodinases in maintaining the euthyroid state and the role of genetic polymorphisms in the deiodinase genes that would affect thyroid hormone concentrations in both blood and tissues. One such polymorphism in the D2 gene, Thr92Ala, is associated with reduced T4 to T3 activation in skeletal muscle and thyroid, linked to obesity and alterations in thyroid-pituitary feedback, and in responses to thyroid hormone treatment. Although our professional organizations continue to recommend L-T4 alone for the treatment of hypothyroidism, the possibility of a D2 gene polymorphism should be considered in patients on L-T4 monotherapy who continue to complain of fatigue in spite of dosage achieving low normal serum thyroid stimulating hormone levels. A suggestive clue to the presence of this polymorphism could be a higher than normal free T4/free T3 ratio. Clinicians could consider adding T3 as a therapeutic trial in selected patients. Future well controlled clinical trials will be required to more fully resolve the controversy.

Record of Operations Against Soviet Russia on Northern and Western Fronts of Manchuria, and in Northern Korea (August 1945)

Science.gov (United States)

1954-09-01

Col Masao Segawa Col Kaoru Okano Col Shigeru Imada - Maj Akizo Yokoyama Capt Toyonobu Kondo Maj Toru Mi tano - Lt Col Sen Nagai - 1st Lt Ichiro...nchurians, and its commander, 1st Lieutenant Ishikawa , was killed. The only unit of the division to engage in action--the 5th Company of the 24lst...Lieutenant: 161 Irie, Major: 43 Ishikawa , 1st Lt: 160 Itung River: 15, 37, 42-43 Iwai, Lt Gen, Torajiro (Cmdr lOath Div): 143, 147-48, 154 Japanese

Sampling and Data Gathering Strategies for Future USAF Anthropometry

Science.gov (United States)

1976-02-01

1973 USAF 1946 27 178.13 195u 23 178.49 - 0.36 cm It is difficult in light of data such as these to accept the ikea that changes in stature are solely...studying the size and shape factors which differentiate the successful from the unsuccessful ejectors. B0 .1," The official end of USAF activities in

Implementation of LT codes based on chaos

International Nuclear Information System (INIS)

Zhou Qian; Li Liang; Chen Zengqiang; Zhao Jiaxiang

2008-01-01

Fountain codes provide an efficient way to transfer information over erasure channels like the Internet. LT codes are the first codes fully realizing the digital fountain concept. They are asymptotically optimal rateless erasure codes with highly efficient encoding and decoding algorithms. In theory, for each encoding symbol of LT codes, its degree is randomly chosen according to a predetermined degree distribution, and its neighbours used to generate that encoding symbol are chosen uniformly at random. Practical implementation of LT codes usually realizes the randomness through pseudo-randomness number generator like linear congruential method. This paper applies the pseudo-randomness of chaotic sequence in the implementation of LT codes. Two Kent chaotic maps are used to determine the degree and neighbour(s) of each encoding symbol. It is shown that the implemented LT codes based on chaos perform better than the LT codes implemented by the traditional pseudo-randomness number generator. (general)

Mutational analysis of COL1A1 and COL1A2 genes among Estonian osteogenesis imperfecta patients.

Science.gov (United States)

Zhytnik, Lidiia; Maasalu, Katre; Reimann, Ene; Prans, Ele; Kõks, Sulev; Märtson, Aare

2017-08-15

Osteogenesis imperfecta (OI) is a rare bone disorder. In 90% of cases, OI is caused by mutations in the COL1A1/2 genes, which code procollagen α1 and α2 chains. The main aim of the current research was to identify the mutational spectrum of COL1A1/2 genes in Estonian patients. The small population size of Estonia provides a unique chance to explore the collagen I mutational profile of 100% of OI families in the country. We performed mutational analysis of peripheral blood gDNA of 30 unrelated Estonian OI patients using Sanger sequencing of COL1A1 and COL1A2 genes, including all intron-exon junctions and 5'UTR and 3'UTR regions, to identify causative OI mutations. We identified COL1A1/2 mutations in 86.67% of patients (26/30). 76.92% of discovered mutations were located in the COL1A1 (n = 20) and 23.08% in the COL1A2 (n = 6) gene. Half of the COL1A1/2 mutations appeared to be novel. The percentage of quantitative COL1A1/2 mutations was 69.23%. Glycine substitution with serine was the most prevalent among missense mutations. All qualitative mutations were situated in the chain domain of pro-α1/2 chains. Our study shows that among the Estonian OI population, the range of collagen I mutations is quite high, which agrees with other described OI cohorts of Northern Europe. The Estonian OI cohort differs due to the high number of quantitative variants and simple missense variants, which are mostly Gly to Ser substitutions and do not extend the chain domain of COL1A1/2 products.

Kinetic studies on binding of thyroid hormones (L-T3 and L-T4) to the receptors of lymphocyte cells isolated from uraemia subjects

International Nuclear Information System (INIS)

Al-Sultani, A.S.J.

1989-01-01

The levels of L-T 3 , L-T 4 and TSH in uremic sera have been measured by (RIA), and shown to have a decrease in both L-T 3 and L-T 4 levels with normal level of TSH for most specimens used in this study. Kinetics properties for binding of thyroid hormones L-T 3 and L-T 4 with nuclear receptors of human lymphocyte cells extracted from uremic patient have been studied and compared this result with control and hypothyroidism subjects and we obtained that uremic condition have a large effect on these nuclear receptors properties. Dissociation constant (K d ) and maximal binding capacity (MBC) of both L-T 3 and L-T 4 with these nuclear receptors have been determined, and we obtained that uremic condition did not affect on (K d ) values for both L-T 3 and L-T 4 but it affected on (MBC) values compared with normal subject. 8 tabs.; 25 figs.; 203 refs

Bronislaw Malinowski’nin Kültür Teorisi

OpenAIRE

Aman, Fatih

2014-01-01

Bir toplumu anlamak o toplumun kültürünü anlamaya bağlıdır. Özellikle “her toplumun kendine özgü olduğu” gerçeği kültür konusunda birçok araştırmanın yapılmasını sağlamış ve kültürel araştırmaları daha da ilginç kılmıştır. Bu araştırmalardan birisi de Bronislaw Malinowski’ye aittir. O, kültürün kökeniyle ilgilenmiş, kültürün fonksiyonlarını tanımlamış, kültürel araştırma tekniklerini ilk defa bilimsel ölçütlerle ortaya koymuş ve eleştirileriyle toplamda bir kültür teorisi oluşturmuştur. Malin...

An Assessment of the USAFE School Board Test Program

Science.gov (United States)

2002-07-01

2000. USAFE Strategic Plan on Dependent Education, Headquarters, USAFE, October 1998. Vajont ES, http://www.vajo-es.eu.odedodea.edu/ Vogelweh ES...Pordenone ES K–6 109 2/2/0 Vajont ES K–6 157 2/2/0 Aviano AB 4 1,365 11/11/1 4/4/1 10/0 Incirlik ES K–6 475 3/3/0 Incirlik HS 7–12 217...Aviano) K–6 109 AF SB VAJONT ES (Aviano) K–6 157 AVIANO ES K–6 629 AVIANO HS 7–12 470 VICENZA ES K–6 480 A IAC VICENZA HS 7–12 251

Mutation Analysis of COL1A1 and COL1A2 in Fetuses with Osteogenesis Imperfecta Type II/III.

Science.gov (United States)

Wang, Wenbo; Wu, Qichang; Cao, Lin; Sun, Li; Xu, Yasong; Guo, Qiwei

2015-01-27

Aim: To analyze COL1A1/2 mutations in prenatal-onset OI for determine the proportion of mutations in type I collagen genes among prenatal onset OI and to provide additional data for genotype-phenotype analyses. Material and Methods: Ten cases of severe fetal short-limb dwarfism detected by antenatal ultrasonography were referred to our center. Before the termination of pregnancy, cordocentesis was performed for fetal karyotype and COL1A1/2 gene sequencing analysis. Postmortem radiographic examination was performed at all instances for definitive diagnosis. Results: COL1A1 and COL1A2 SNP and mutations were identified in all the cases. Among these, one synonymous SNP and four synonymous SNPs were recognized in COL1A1/2, respectively, seven cases have distinct heterozygous mutations and six new COL1A1/2 gene mutations were identified. Conclusion: There has been substantial progress in the identification of the molecular defects responsible for skeletal dysplasias. With the constant increase in the number of identified mutations in COL1A1 and COL1A2, genotype-phenotype correlation is becoming increasingly pertinent. © 2015 S. Karger AG, Basel.

Translations on Narcotics and Dangerous Drugs No. 294

Science.gov (United States)

1977-04-13

surveillance over the activity of the smugglers. The investigations of Col Mahmud Fahmi and Lt Col ’Isam al-Tarsawi showed that the smuggler, Anwar Ibrahim...Mahmud Fahmi , It Col ’Isam al-Tarsawi, Lt Col Tariq Salim and It Col Muhammad Sha’rawi raided "the Jinn’s" house in ’Abidin and seized four large...Yesterday a car dealer and his associate were arraigned in court on charges of transporting 205 kg of hashish. The first accused was Reza

Mutation analysis of COL4A3 and COL4A4 genes in a Chinese ...

Indian Academy of Sciences (India)

2016-10-24

，. People's Republic of China ... mutations in COL4A3 and COL4A4 genes which encode type IV collagen α3 and α4 chainsrespectively can .... hematuria and evidence for activation of the unfolded protein response. Focal and ...

Low expression of CysLT1R and high expression of CysLT2R mediate good prognosis in colorectal cancer

DEFF Research Database (Denmark)

Magnusson, Cecilia; Mezhybovska, Maryna; Lörinc, Ester

2010-01-01

Colorectal cancer is the third most common cancer type in the Western world. In search of new treatment possibilities, the inflammation mediators, know as cysteinyl leukotrienes (CysLTs), have been shown to regulate intestinal epithelial cell survival and proliferation via the CysLT(1)R, and cell...... microarray of 329 colorectal patients. We found that high nuclear expression of CysLT(1)R is associated with a poor prognosis, whereas high nuclear expression of CysLT(2)R is associated with a good prognosis. We also observed that patients with colorectal tumours characterised by high CysLT(1)R but low Cys...

2015 USAFA Research Report: Discover Falcon Innovation

Science.gov (United States)

2015-01-01

geotechnical, structures, environmental and construction. First, in the geotechnical division, Lt Col Chris Senseney is researching “Computational Modeling of...communicate. “It’s a new area for us,” said Lt Col Chris McClernon, director of the center. “We started it last summer, because we know communications...Sales de Souza, Major Mark Braun, 1st Lt Melanie Ortiz, 1st Lt Seth Rodgers and Cadet 1st Class Jordan Higgins are working on a research project

Introducing Leo LT - the "three-headed lion"

Index Scriptorium Estoniae

2008-01-01

Leedu valitsus kiitis heaks seaduseparandused, mille tulemusena saab võtta tuumajaama ehitaja, investeerimis- ja energiafirma Leo LT strateegilise tähtsusega firmade hulka. Kui parlament valitsuse ettepanekud heaks kiidab, saab riik 61,7% Leo LT ja 38,3% NDX Energija aktsiatest

TEO LT hakkab kaotama atraktiivsust / Romet Kreek

Index Scriptorium Estoniae

Kreek, Romet, 1972-

2011-01-01

Autori sõnul on Leedu telekomiettevõtte TEO LT puhul raske oodata, et käive ja kasum kasvaksid, parimal juhul on need stabiilsed. Osalt on selle põhjuseks tehnoloogilised muutused, osalt suuromaniku poliitika. TEO LT I kvartali majandusnäitajatest

Mutation analysis of the COL1A1 and COL1A2 genes in Vietnamese patients with osteogenesis imperfecta.

Science.gov (United States)

Ho Duy, Binh; Zhytnik, Lidiia; Maasalu, Katre; Kändla, Ivo; Prans, Ele; Reimann, Ene; Märtson, Aare; Kõks, Sulev

2016-08-12

The genetics of osteogenesis imperfecta (OI) have not been studied in a Vietnamese population before. We performed mutational analysis of the COL1A1 and COL1A2 genes in 91 unrelated OI patients of Vietnamese origin. We then systematically characterized the mutation profiles of these two genes which are most commonly related to OI. Genomic DNA was extracted from EDTA-preserved blood according to standard high-salt extraction methods. Sequence analysis and pathogenic variant identification was performed with Mutation Surveyor DNA variant analysis software. Prediction of the pathogenicity of mutations was conducted using Alamut Visual software. The presence of variants was checked against Dalgleish's osteogenesis imperfecta mutation database. The sample consisted of 91 unrelated osteogenesis imperfecta patients. We identified 54 patients with COL1A1/2 pathogenic variants; 33 with COL1A1 and 21 with COL1A2. Two patients had multiple pathogenic variants. Seventeen novel COL1A1 and 10 novel COL1A2 variants were identified. The majority of identified COL1A1/2 pathogenic variants occurred in a glycine substitution (36/56, 64.3 %), usually serine (23/36, 63.9 %). We found two pathogenic variants of the COL1A1 gene c.2461G > A (p.Gly821Ser) in four unrelated patients and one, c.2005G > A (p.Ala669Thr), in two unrelated patients. Our data showed a lower number of collagen OI pathogenic variants in Vietnamese patients compared to reported rates for Asian populations. The OI mutational profile of the Vietnamese population is unique and related to the presence of a high number of recessive mutations in non-collagenous OI genes. Further analysis of OI patients negative for collagen mutations, is required.

CrossTalk: The Journal of Defense Software Engineering. Volume 24, Number 1, Jan/Feb 2011

Science.gov (United States)

2011-02-01

Aircraft Sustainment Group Tony Henderson 309th Software Maintenance Group Lt. Col. Brian Hermann, Ph.D. Defense Information Systems Agency Lt. Col...Solutions, Inc. Gordon Sleve Robbins Gioia LLC Larry Smith Software Technology Support Center Dr. John Sohl Weber State University Elizabeth Starrett OO-ALC

Mutation analysis of COL4A3 and COL4A4 genes in a Chinese

Indian Academy of Sciences (India)

Autosomal dominant Alport syndrome (ADAS) accounts for 5% of all cases of Alport syndrome (AS), a primary basement membrane disorder arising from mutations in genes encoding the type IV collagen protein family.Mutationsin COL4A3 and COL4A4 genes were reported to be associated with ADAS. In this study, clinical ...

A Systematic Review of Clinical Practice Guidelines' Recommendations on Levothyroxine Therapy Alone versus Combination Therapy (LT4 plus LT3) for Hypothyroidism.

Science.gov (United States)

Kraut, Eyal; Farahani, Pendar

2015-12-04

Patients with hypothyroidism are increasingly enquiring about the benefit of using combination therapy of levothyroxine (LT4) and liothyronine (LT3) as a potential treatment for hypothyroidism. Combination therapy, however, remains controversial. The purpose of this study was to systematically review available hypothyroidism treatment recommendations from clinical practice guidelines from around the world to identify the consensus regarding combination therapy. Clinical practice guidelines were obtained from searches of PubMed, EMBASE, and MEDLINE, using several combinations of MeSH terms. The search was limited to clinical guidelines in English-language publications, published between January 1, 1990 and May 1, 2015. A quantitative approach was utilized for data synthesis. Thirteen guidelines were identified, including three regarding pregnancy, two regarding pediatric populations and eight regarding adult populations. There were six guidelines from North America, four guidelines from Europe and three guidelines from South America. Twelve of the guidelines were published after 2010. Nine guidelines addressed combination therapy of LT4 plus LT3, and all nine concluded that LT4 therapy alone is the standard of care, with insufficient evidence to recommend widespread combination therapy. Only the 2012 ETA Guidelines and the 2015 BTA Guidelines concluded that combination therapy could be used, although only in certain circumstances and as an experimental treatment. This systematic review illustrates that clinical practice guidelines worldwide do not recommend and do not support routine use of combination LT4 and LT3 therapy to treat hypothyroidism.

Determining Effective Leadership Behaviors for USAF Company Grade Officers

National Research Council Canada - National Science Library

Berry, Warren

1998-01-01

... (Yukl and Van Fleet, 1986). The purpose of this investigation is to determine the critical leadership behaviors required by junior officers at the direct level of responsibility in the USAF, and to determine differences...

Effective Measurement of Reliability of Repairable USAF Systems

Science.gov (United States)

2012-09-01

ix I. Introduction ...RELIABILITY OF REPAIRABLE USAF SYSTEMS I. Introduction General Issue The United States relies on complex systems to protect and project the national...Under Secretary of Defense for Acquisition, Technology, and Logistics, Implementing a Lifecycle Mangement Framework, Washington DC, DC: DoD, 2008

Joint Department of Defense, Advance Planning Briefing to Industry, Held at Kissimmee, Florida, on 30 April - 2 May 1996

Science.gov (United States)

1996-05-01

Systems 17 Motion Bases Upgraded Aero Misc > Contact Info • AMC, Lt Col Letica , Phone (618) 256-5696 L8 Upgrade Flow Plan Advance Planning Briefing...Projected Schedule: FY 05 > Requirement Document: AMMP >• Projected Program Size: ? < $40M > Funding Status/Stability: > POCs: • AMC, Lt Col Letica

Increasing doses effect of L-T4 and L-T3 in the hypothalamus - hypophysis - thyroid in patients carrier of congenital and acquired hypothyroidism

International Nuclear Information System (INIS)

Cavaliere, H.

1987-01-01

The pituitary and peripheral response to L-T4 and L-T3 therapy were studied in 12 patients with congenital goitrous hypothyroidism, in 10 patients with an ectopic thyroid and onset of hypothyroidism at 3-8 years of age, and in 6 patients with adult-onset hypothyroidism, after they had had their chronic thyroid hormone replacement therapy discontinued for 30 days. They were first treated with increasing L-T4 (0.1, 0.2, and 0.4 mg daily) followed by L-T3 (0.05 and 0.2 mg daily) after stopping thyroid medication for another month. Ten normal subjects were treated identically. Since all patients received similar doses of thyroid hormones (μg/Kg of body weight) and had similar serum levels of T4 and T3 on each dose of L-T4 or L-T3, this paper concludes that congenitally hypothyroid patients have persistent pituitary resistance, but no peripheral resistance, to thyroid hormone. (author)

Rotavirus 2/6 Viruslike Particles Administered Intranasally with Cholera Toxin, Escherichia coli Heat-Labile Toxin (LT), and LT-R192G Induce Protection from Rotavirus Challenge

Science.gov (United States)

O’Neal, Christine M.; Clements, John D.; Estes, Mary K.; Conner, Margaret E.

1998-01-01

We have shown that rotavirus 2/6 viruslike particles composed of proteins VP2 and VP6 (2/6-VLPs) administered to mice intranasally with cholera toxin (CT) induced protection from rotavirus challenge, as measured by virus shedding. Since it is unclear if CT will be approved for human use, we evaluated the adjuvanticity of Escherichia coli heat-labile toxin (LT) and LT-R192G. Mice were inoculated intranasally with 10 μg of 2/6-VLPs combined with CT, LT, or LT-R192G. All three adjuvants induced equivalent geometric mean titers of rotavirus-specific serum antibody and intestinal immunoglobulin G (IgG). Mice inoculated with 2/6-VLPs with LT produced significantly higher titers of intestinal IgA than mice given CT as the adjuvant. All mice inoculated with 2/6-VLPs mixed with LT and LT-R192G were totally protected (100%) from rotavirus challenge, while mice inoculated with 2/6-VLPs mixed with CT showed a mean 91% protection from challenge. The availability of a safe, effective mucosal adjuvant such as LT-R192G will increase the practicality of administering recombinant vaccines mucosally. PMID:9525668

Feedback in LT codes for prioritized and non-prioritized data

DEFF Research Database (Denmark)

Sørensen, Jesper Hemming; Popovski, Petar; Østergaard, Jan

2012-01-01

In this paper feedback in LT codes is investigated. The considered type of feedback is acknowledgments, where information on which symbols have been decoded is given to the transmitter. Our analysis reveals that acknowledgments has a very low potential in LT codes with standard degree distributions....... Motivated by this, we analyze the impact of acknowledgments on multi-layer LT codes. In this case, feedback proves advantageous. By using only a single feedback message, it is possible to achieve a significant performance improvement compared to traditional LT codes....

Tuberculose du col utérin simulant un cancer du col utérin : à propos ...

African Journals Online (AJOL)

Tuberculose du col utérin simulant un cancer du col utérin : à propos d'un cas, au Centre Hospitalier Universitaire Souro Sanou de Bobo-Dioulasso, Burkina Faso. A. Dembélé, V. Konségré, E. Birba, D.A. Somé, H. Zamané, A.S. Ouédraogo, S. Kiemtoré, S. Ouattara, A. Lamien-Sanou, M. Bambara, B. Bonané/Thiéba ...

The Automated DC Parameter Testing of GaAs MESFETs Using the Singer Automatic Integrated Circuit Test System.

Science.gov (United States)

1980-09-01

USING THE SINGER AUTOMATIC INTEGRATED CIRCUIT TEST SYSTEM, THOMAS L. HARPER AFIT/EE/GE/80- 7 Ist LT USAF -- -- - - __ AFIT/EE/GE/80-7 THE AUTOMATED DC...THOMAS L. HARPER ist Lt USAF Graduate Electrical Engineering September 1980 it’ Codes A _ _ _ J PREFACE This report is in support of the ongoing effort in...8217.-- I *t -1 ,p - tUel-, ir. ( /.s , j Yf) L) b ..... l P i:. +’ ,T i~: ",,’+l l L V i i ,’b : O Iil r, P V 47 C’+t ( ’ I ViH 47 V ’L 4 £, ,.;l 1 , h

Rotavirus 2/6 Viruslike Particles Administered Intranasally with Cholera Toxin, Escherichia coli Heat-Labile Toxin (LT), and LT-R192G Induce Protection from Rotavirus Challenge

OpenAIRE

O’Neal, Christine M.; Clements, John D.; Estes, Mary K.; Conner, Margaret E.

1998-01-01

We have shown that rotavirus 2/6 viruslike particles composed of proteins VP2 and VP6 (2/6-VLPs) administered to mice intranasally with cholera toxin (CT) induced protection from rotavirus challenge, as measured by virus shedding. Since it is unclear if CT will be approved for human use, we evaluated the adjuvanticity of Escherichia coli heat-labile toxin (LT) and LT-R192G. Mice were inoculated intranasally with 10 μg of 2/6-VLPs combined with CT, LT, or LT-R192G. All three adjuvants induced ...

Detection of COL III in Parchment by Amino Acid Analysis

DEFF Research Database (Denmark)

Vestergaard Poulsen Sommer, Dorte; Larsen, René

2016-01-01

Cultural heritage parchments made from the reticular dermis of animals have been subject to studies of deterioration and conservation by amino acid analysis. The reticular dermis contains a varying mixture of collagen I and III (COL I and III). When dealing with the results of the amino acid...... analyses, till now the COL III content has not been taken into account. Based on the available amino acid sequences we present a method for determining the amount of COL III in the reticular dermis of new and historical parchments calculated from the ratio of Ile/Val. We find COL III contents between 7...... and 32 % in new parchments and between 0.2 and 40 % in the historical parchments. This is consistent with results in the literature. The varying content of COL III has a significant influence on the uncertainty of the amino acid analysis. Although we have not found a simple correlation between the COL...

Thermoelectric coefficient L(T) of polycrystalline silver doped BSCCO samples

International Nuclear Information System (INIS)

Rodriguez, J.E.; Marino, A.

1998-01-01

We present a study of the thermoelectric coefficient L(T) of polycrystalline silver doped BSCCO samples. The quantity L(T) relates the thermoelectric coefficient S(T) with the electrical conductivity σ (T) and gives an indication of the influence of the order parameter fluctuations (OPF) on S(T) in the mean field region (Mfr). The results of L(T) indicate that the critical behavior of S(T) above the superconducting transition is not only driven by σ (T). These results suggest that in the Mfr, L(T) is affected by thermodynamic fluctuations of the superconducting order parameter (OPF). The OPF effects show a two-dimensional (2D) character in the entire Mfr. (Author)

The transmembrane collagen COL-99 guides longitudinally extending axons in C. elegans.

Science.gov (United States)

Taylor, Jesse; Unsoeld, Thomas; Hutter, Harald

2018-06-01

We have identified the transmembrane collagen, COL-99, in a genetic screen for novel genes involved in axon guidance in the nematode C. elegans. COL-99 is similar to transmembrane collagens type XIII, XXIII and XXV in vertebrates. col-99 mutants exhibit guidance defects in axons extending along the major longitudinal axon tracts, most prominently the left ventral nerve cord (VNC). COL-99 is expressed in the hypodermis during the time of axon outgrowth. We provide evidence that a furin cleavage site in COL-99 is essential for function, suggesting that COL-99 is released from the cells producing it. Vertebrate homologs of COL-99 have been shown to be expressed in mammalian nervous systems and linked to various neurological disease but have not been associated with guidance of extending neurons. col-99 acts genetically with the discoidin domain receptors ddr-1 and ddr-2, which are expressed by neurons affected in col-99 mutants. Discoidin domain receptors are activated by collagens in vertebrates. DDR-1 and DDR-2 may function as receptors for COL-99. Our results establish a novel role for a transmembrane collagen in axonal guidance and asymmetry establishment of the VNC. Copyright © 2018 Elsevier Inc. All rights reserved.

Trigon Farming ostis Kaiu LT / Margus Mikomägi

Index Scriptorium Estoniae

Mikomägi, Margus, 1956-

2006-01-01

Raplamaalt suuruselt neljas põllumajandusühistu Kaiu LT müüdi üldkoosoleku otsusega ühel häälel firmale Trigon Farming. Kaiu LT nõukogu esimees on viimased kolm aastat olnud Riigikogu liige Tiit Tammsaar. Lisa: Trigon Tarming investeerib põllumajandusse

Assessment of Head-Injured Aircrew: Comparison of FAA and USAF Procedures

National Research Council Canada - National Science Library

Fiedler, Edna

2001-01-01

.... The purpose of this paper is to first explain the major differences in missions between the USAF and the FAA, and then outline these procedures and rules, note their similarities and differences...

Critical early roles for col27a1a and col27a1b in zebrafish notochord morphogenesis, vertebral mineralization and post-embryonic axial growth.

Science.gov (United States)

Christiansen, Helena E; Lang, Michael R; Pace, James M; Parichy, David M

2009-12-29

Fibrillar collagens are well known for their links to human diseases, with which all have been associated except for the two most recently identified fibrillar collagens, type XXIV collagen and type XXVII collagen. To assess functions and potential disease phenotypes of type XXVII collagen, we examined its roles in zebrafish embryonic and post-embryonic development. We identified two type XXVII collagen genes in zebrafish, col27a1a and col27a1b. Both col27a1a and col27a1b were expressed in notochord and cartilage in the embryo and early larva. To determine sites of type XXVII collagen function, col27a1a and col27a1b were knocked down using morpholino antisense oligonucleotides. Knockdown of col27a1a singly or in conjunction with col27a1b resulted in curvature of the notochord at early stages and formation of scoliotic curves as well as dysmorphic vertebrae at later stages. These defects were accompanied by abnormal distributions of cells and protein localization in the notochord, as visualized by transmission electron microscopy, as well as delayed vertebral mineralization as detected histologically. Together, our findings indicate a key role for type XXVII collagen in notochord morphogenesis and axial skeletogenesis and suggest a possible human disease phenotype.

Childhood presentation of COL4A1 mutations

NARCIS (Netherlands)

Shah, S.; Ellard, S.; Kneen, R.; Lim, M.; Osborne, N.; Rankin, J.; Stoodley, N.; van der Knaap, M.S.; Whitney, A.; Jardine, P.

2012-01-01

Aim To describe the clinical and radiological features of four new families with a childhood presentation of COL4A1 mutation. Method We retrospectively reviewed the clinical presentation. Investigations included radiological findings and COL4A1 mutation analysis of the four cases. Affected family

Childhood presentation of COL4A1 mutations

NARCIS (Netherlands)

Shah, Siddharth; Ellard, Sian; Kneen, Rachel; Lim, Ming; Osborne, Nigel; Rankin, Julia; Stoodley, Neil; van der Knaap, Marjo; Whitney, Andrea; Jardine, Philip

2012-01-01

To describe the clinical and radiological features of four new families with a childhood presentation of COL4A1 mutation. We retrospectively reviewed the clinical presentation. Investigations included radiological findings and COL4A1 mutation analysis of the four cases. Affected family members were

Col4a1 mutations cause progressive retinal neovascular defects and retinopathy.

Science.gov (United States)

Alavi, Marcel V; Mao, Mao; Pawlikowski, Bradley T; Kvezereli, Manana; Duncan, Jacque L; Libby, Richard T; John, Simon W M; Gould, Douglas B

2016-01-27

Mutations in collagen, type IV, alpha 1 (COL4A1), a major component of basement membranes, cause multisystem disorders in humans and mice. In the eye, these include anterior segment dysgenesis, optic nerve hypoplasia and retinal vascular tortuosity. Here we investigate the retinal pathology in mice carrying dominant-negative Col4a1 mutations. To this end, we examined retinas longitudinally in vivo using fluorescein angiography, funduscopy and optical coherence tomography. We assessed retinal function by electroretinography and studied the retinal ultrastructural pathology. Retinal examinations revealed serous chorioretinopathy, retinal hemorrhages, fibrosis or signs of pathogenic angiogenesis with chorioretinal anastomosis in up to approximately 90% of Col4a1 mutant eyes depending on age and the specific mutation. To identify the cell-type responsible for pathogenesis we generated a conditional Col4a1 mutation and determined that primary vascular defects underlie Col4a1-associated retinopathy. We also found focal activation of Müller cells and increased expression of pro-angiogenic factors in retinas from Col4a1(+/Δex41)mice. Together, our findings suggest that patients with COL4A1 and COL4A2 mutations may be at elevated risk of retinal hemorrhages and that retinal examinations may be useful for identifying patients with COL4A1 and COL4A2 mutations who are also at elevated risk of hemorrhagic strokes.

A novel formulation of L-thyroxine (L-T4) reduces the problem of L-T4 malabsorption by coffee observed with traditional tablet formulations.

Science.gov (United States)

Vita, Roberto; Saraceno, Giovanna; Trimarchi, Francesco; Benvenga, Salvatore

2013-02-01

The purpose of this work is to evaluate if the coffee-associated malabsorption of tablet levothyroxine (L-T4) is reduced by soft gel capsule. We recruited 8 patients with coffee-associated L-T4 malabsorption including one hypothyroid patient. For 6 months, the patients were switched to the capsule maintaining the L-T4 daily dose. Patients took the capsule with water, having coffee 1 h later (proper habit, PH) on days 1-90, or with coffee ≤ 5 min later (improper habit, IH) on days 91-180. After 6 months, 2 patients volunteered for an acute loading test of 600 μg L-T4 (capsule) ingested with water (PH) or with coffee (IH). In the single hypothyroid patient, the post-switch TSH ranged 0.06-0.16 mU/L (PH) versus 5.8-22.4 mU/L pre-switch (PH) and 0.025-0.29 mU/L (IH) versus 26-34 mU/L pre-switch (IH). In the other 7 patients, post-switch TSH was 0.41 ± 0.46 (PH) versus 0.28 ± 0.20 pre-switch (PH) (P = 0.61) and 0.34 ± 0.30 (IH) versus 1.23 ± 1.47 pre-switch (IH) (P coffee influenced L-T4 pharmacokinetics minimally. Soft gel capsules can be used in patients who are unable/unwilling to change their IH of taking L-T4.

Strategic Studies Quarterly. Volume 2, Number 3, Fall 2008

Science.gov (United States)

2008-01-01

Managing Editor Betty R. Littlejohn, Editorial Assistant Jerry L. Gantt, Content Editor Sherry Terrell , Editorial Assistant Steven C. Garst...factsheet.asp?id=107 . Ibid. 9. Lt Col Sebastian M. Convertino II, CDR Lou Anne DeMattei, and Lt Col Tammy Knierim, Flying and Fighting in...PhD, Editor-in-Chief L. Tawanda Eaves, Managing Editor Betty R. Littlejohn, Editorial Assistant Jerry L. Gantt, Content Editor Sherry Terrell

Airborne Operations in World War II, European Theater

Science.gov (United States)

1956-09-01

zones might have to be changed to suit the ground situation. , . Caroes were to consist of 6 bundles in each plane and six more carried in pararacks in...III, and Pt IV; Hq 508th PIR, SITREP, 18 Sep 44, in Box 2022, Item 2037; Itr, Lt Col L. G. Mendez to Lt Col Krebs, 8 Oct 44, in hist file 440th TC Gp

Heritage, Image and Identity: The Evolution of USAF Leadership

Science.gov (United States)

2011-02-16

up-in-coming “ Generation Z ” (also known as the “Net or Digital Generation”), which is the most connected and high-tech generation ever seen. 40...for future RPA warrior leaders. 43 The USAF has already set the ground work to position “ Generation Z ” RPA pilots for future senior leadership

An Empirical Investigation of USAF Logistics Readiness Officer Mission Sets

Science.gov (United States)

2013-03-01

xi I. Introduction ...USAF LOGISTICS READINESS OFFICER MISSION SETS I. Introduction Background “While its mission and purpose are constant, the logistics...one can see that information mangement and business acumen are near the top in each one. Acquisitions and process improvement were the KSAs with

EuroCirCol: A key to New Physics

CERN Multimedia

Johannes Gutleber

2015-01-01

Monday 1 June saw the start of EuroCirCol, the EC-funded part of the FCC study that will develop the conceptual design for an energy-frontier hadron collider.   Attendees at the EuroCirCol meeting at CERN. The EuroCirCol kick-off event at CERN on 2 to 4 June brought together 62 participants to constitute governance bodies, commit to the project plan and align the organisation, structures and processes of 16 institutions from 10 countries. The goal of the project is to conceive a post-LHC research infrastructure around a 100 km circular energy-frontier hadron collider capable of reaching 100 TeV collisions. The project officially started on 1 June and will run for four years. The total estimated budget of 11.2 MEUR includes a 2.99 MEUR contribution from the Horizon 2020 programme dedicated to the development of new world-class research infrastructures. EuroCirCol will deliver a design for a hadron collider as part of the broader Future Circular Collider (FCC) study. It will provide input to an...

Joint Task Force - Port Opening: Can this Emerging Capability Expedite Operational Objectives Throughout the Range of Military Operations?

Science.gov (United States)

2009-10-26

for Acquisition, Technology, and Logistics, 30 July 2007). 16 Craig Koontz , ―U.S. Transportation Command,‖ PowerPoint, 23 September 2009, Newport, RI...Support Group. To Lt Col Michael W. Pratt, Naval War College. Memorandum, 30 September 2009. Koontz , Craig. ―U.S. Transportation Command...PowerPoint. 23 September 2009. 22 Koontz , Craig. Contractor/Advisor to CDR U.S. Transportation Command. To Lt Col Michael W. Pratt, 28

Chief of Staff of the Air Force

Science.gov (United States)

> CSAF Posture Statement USAF Posture Statement FY18 Featured Videos &amp;amp;amp;amp ;amp;amp;amp;amp;amp;lt;!-- &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;br /& ;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp

Design and Analysis of LT Codes with Decreasing Ripple Size

DEFF Research Database (Denmark)

Sørensen, Jesper Hemming; Popovski, Petar; Østergaard, Jan

2012-01-01

In this paper we propose a new design of LT codes, which decreases the amount of necessary overhead in comparison to existing designs. The design focuses on a parameter of the LT decoding process called the ripple size. This parameter was also a key element in the design proposed in the original...... work by Luby. Specifically, Luby argued that an LT code should provide a constant ripple size during decoding. In this work we show that the ripple size should decrease during decoding, in order to reduce the necessary overhead. Initially we motivate this claim by analytical results related...... to the redundancy within an LT code. We then propose a new design procedure, which can provide any desired achievable decreasing ripple size. The new design procedure is evaluated and compared to the current state of the art through simulations. This reveals a significant increase in performance with respect...

Mainosideoista sähköisiin sisältöihin : sisältöstrategian merkitys mainostoimistojen digitaalisissa palveluissa

OpenAIRE

Pasanen, Riikka

2012-01-01

Sisältöstrategia on suunnittelukäytäntö, jonka tähtää liiketoiminnallisiin tavoitteisiin vaikuttavan verkkosisällön avulla. Tutkimuksessa tarkastellaan hyvän verkkosisällön kriteerejä ja pohditaan, voisiko mainostoimisto ottaa oppia sisältöstrategian käytännöistä ja tarjota sen avulla parempaa palvelua asiakkailleen. Tutkimusta varten on haastateltu neljää mainonnan strategisen suunnittelun ja asiakasjohdon ammattilaista, joiden näkemyksiä on peilattu tutkijan pitkään kokemukseen alalta sekä ...

Hybrid Threat Center of Gravity Analysis: Cutting the Gordian Knot

Science.gov (United States)

2016-04-04

19b. TELEPHONE NUMBER (Include area code) 04/04/2016 Master’s Thesis 22-7-2015 to 04-04-2016 HYBRID THREAT CENTER OF GRAVITY ANALYSIS: CUTTING THE...CENTER OF GRAVITY ANALYSIS: CUTTING THE GORDIAN KNOT By Michael D. Reilly LtCol, USMC Intentionally left blank...HYBRID THREAT CENTER OF GRAVITY ANALYSIS: CUTTING THE GORDIAN KNOT By Michael D. Reilly LtCol, USMC A paper submitted to the Faculty of the Joint Advanced

The Application of LT-Table in TRIZ Contradiction Resolving Process

Science.gov (United States)

Wei, Zihui; Li, Qinghai; Wang, Donglin; Tian, Yumei

TRIZ is used to resolve invention problems. ARIZ is the most powerful systematic method which integrates all of TRIZ heuristics. Definition of ideal final result (IFR), identification of contradictions and resource utilization are main lines of ARIZ. But resource searching of ARIZ has fault of blindness. Alexandr sets up mathematical model of transformation of the hereditary information in an invention problem using the theory of catastrophes, and provides method of resource searching using LT-table. The application of LT-table on contradiction resolving is introduced. Resource utilization using LT-table is joined into ARIZ step as an addition of TRIZ, apply this method in separator paper punching machine design.

Development and Employment of Fixed-Wing Gunships 1962-1972

Science.gov (United States)

1982-01-01

Carl Berger. Mr. Eugene P. Sagstetter, Mary F. Loughlin, and Vanessa D. Allen edited, proofread, and purged the manuscript of the typographical...General Coun.el. USAF Dr. Forrest C. Pogue Lt. General Charles G. Cleveland Smithsonian Institution USAF Commander. Air University. ATC Dr. Edward L...GiUNSHIP I (AC-47) Major Interdiction Areas (Southeast Asia) A NORTH VIETNAM Bar 1 LAOS Steel Tiger THAILAND ’ * Tiger Hound *~ ~T39 VAN DEVELOPMENT OF FIXED

Dietary Lecithin Decreases Skeletal Muscle COL1A1 and COL3A1 Gene Expression in Finisher Gilts

Directory of Open Access Journals (Sweden)

Henny Akit

2016-06-01

Full Text Available The purpose of this study was to investigate the effect of dietary lecithin on skeletal muscle gene expression of collagen precursors and enzymes involved in collagen synthesis and degradation. Finisher gilts with an average start weight of 55.9 ± 2.22 kg were fed diets containing either 0, 4, 20 or 80 g/kg soybean lecithin prior to harvest for six weeks and the rectus abdominis muscle gene expression profile was analyzed by quantitative real-time PCR. Lecithin treatment down-regulated Type I (α1 procollagen (COL1A1 and Type III (α1 procollagen (COL3A1 mRNA expression ( p < 0.05, respectively, indicating a decrease in the precursors for collagen synthesis. The α-subunit of prolyl 4-hydroxylase (P4H mRNA expression also tended to be down-regulated ( p = 0.056, indicating a decrease in collagen synthesis. Decreased matrix metalloproteinase-1 (MMP-1 mRNA expression may reflect a positive regulatory response to the reduced collagen synthesis in muscle from the pigs fed lecithin ( p = 0.035. Lecithin had no effect on tissue inhibitor metalloproteinase-1 (TIMP-1, matrix metalloproteinase-13 (MMP-13 and lysyl oxidase mRNA expression. In conclusion, lecithin down-regulated COL1A1 and COL3A1 as well as tended to down-regulate α-subunit P4H expression. However, determination of muscle collagen content and solubility are required to support the gene functions.

A Shrinking Army in Europe: Can the US Achieve Its Military Strategic Goals Without It?

Science.gov (United States)

2014-06-13

French and was lauded as an operational strength by both French generals and subsequent Armée De Terre after action reviews.153 The joint headquarters...2013): 48. 151LtCol Yannick Legrand and LtCol Emmanuel Durville, “French Update September-October 2013,” Armée de Terre Special Update (October 2013...surveillance and reconnaissance assets (ISR) provided much of the targeting information to French howitzers and aircraft.158 The Armée De Terre also proved

Disasters and Impact of Sleep Quality and Quantity on National Guard Medical Personnel

Science.gov (United States)

2018-04-30

Maryon, T., & Rowan, S. (2018). Using technology to advance the science of nursing research . International Journal of Social Science & Technology, 3(2...Health Sciences , the Department of Defense , or the U.S. Government. TriService Nursing Research (TSNRP). The views of ReadibandT are not necessarily...3216.02_AFl40-402 ~ i College of Nursing 4/11/2018 1 Research Team LtCol (Retired)Denise Smart LtCol Stephanie Rowan, MN. ChiefNurse 149 Medical Group

An Educational Methodology for Enhancing Familiarity with United States Air Force Combat Logistics.

Science.gov (United States)

1983-09-01

8217ON 01 ’ fS 0A^𔃻W en Or Ertered) "- ; .: -? " ’_ ". i. . :~ ~~~~~~~.. .. .. . . ." .i . ,7 - , ’ ’ _:-: . ,_ ,_ _ .- LSSR 44-83 AN EDUCATIONAL... lectures ; personal interviews; conferences; phone calls; books and other publications. Additionally, Lt Col Thomas Harrington and Lt Col James Annesser...manmade hostile environ- Itions ment without suffering an abortive Lmpairment I of its ability to accomplish its designed mission (47:676). Fle

Upper bound on the non-colorability threshold of the 2+p-COL problem

International Nuclear Information System (INIS)

Cruz, R.

2004-10-01

The 2p-COL problem introduced by Walsh, smoothly interpolates between P and NP by mixing together the polynornial 2-coloring problem and the NP complete 3-coloring problem. A natural upper bound on the non colorability of the 2+p-COL problem in min {r-bar 2 /(1 - p)r-bar 3 }, where r-bar 2 and r-bar 3 are the upper bounds on 2-COL and 3-COL thresholds respectively. In this paper we improve this upper-bound for each 0.73 ≤ p 1. This means that for p ≥ 0.73 the 2+p-COL problem does not behave like the 2-COL problem. We use the method developed by Kaporis et al., which combines the concept of legal rigid colorings introduced by Achlioptas and Molloy with the occupancy problem for random allocations of balls into bins. (author)

USAF/SCEEE Summer Faculty Research Program (1979). Volume 2

Science.gov (United States)

1979-12-01

K. Schwarzschild , Math. Phys, Kiasse, Grottingen Nachrichten, p. 41 (1906). . 28-21 I -. 4; 1979 USAF - SCEEE SUMMER FACULTY RESEARCH PROGRAM...Wollam, 1968, p. 57. 22. Richard H. Hall, Organizations Structure and Process (New Jersey: Prentice-Hall, Inc., 2nd ed., 1978). 23. Karl E. Weick, The...The Analysis of the U.S. Army Aircraft Maintenance System, Battelle Memorial Institute, 1970. (AD703839) Weick, Karl E. The Social Psychology of

ColVI myopathies: where do we stand, where do we go?

Directory of Open Access Journals (Sweden)

Allamand Valérie

2011-09-01

Full Text Available Abstract Collagen VI myopathies, caused by mutations in the genes encoding collagen type VI (ColVI, represent a clinical continuum with Ullrich congenital muscular dystrophy (UCMD and Bethlem myopathy (BM at each end of the spectrum, and less well-defined intermediate phenotypes in between. ColVI myopathies also share common features with other disorders associated with prominent muscle contractures, making differential diagnosis difficult. This group of disorders, under-recognized for a long time, has aroused much interest over the past decade, with important advances made in understanding its molecular pathogenesis. Indeed, numerous mutations have now been reported in the COL6A1, COL6A2 and COL6A3 genes, a large proportion of which are de novo and exert dominant-negative effects. Genotype-phenotype correlations have also started to emerge, which reflect the various pathogenic mechanisms at play in these disorders: dominant de novo exon splicing that enables the synthesis and secretion of mutant tetramers and homozygous nonsense mutations that lead to premature termination of translation and complete loss of function are associated with early-onset, severe phenotypes. In this review, we present the current state of diagnosis and research in the field of ColVI myopathies. The past decade has provided significant advances, with the identification of altered cellular functions in animal models of ColVI myopathies and in patient samples. In particular, mitochondrial dysfunction and a defect in the autophagic clearance system of skeletal muscle have recently been reported, thereby opening potential therapeutic avenues.

Effects of 18 months of L-T4 replacement in women with subclinical hypothyroidism.

LENUS (Irish Health Repository)

Adrees, M

2012-02-01

CONTEXT: Some of the cardiovascular and renal abnormalities seen in overt hypothyroidism have also been reported in subclinical hypothyroidism (SCH). Short-term L-T4 replacement in SCH improves cardiovascular risk markers and reduces carotid intima-media thickness (CIMT), a surrogate marker of atherosclerosis. The haemodynamic and renal effects of L-T4 replacement in SCH are poorly understood. OBJECTIVES: To compare cardiovascular risk factors and renal variables in women with SCH and normal women. To study the effects of L-T4 replacement in SCH subjects on these variables and on structural and functional changes in common carotid and brachial arteries. DESIGN: Fifty-six women with SCH before and after L-T4 replacement for 18 months and 56 normal women of similar age distribution were studied. Blood Pressure (BP), plasma lipids and homocysteine were measured and renal function evaluated [estimation of glomerular filtration rate (eGFR) using standard equations and measurement of serum Cystatin-C] in women with SCH before and after 18 months of l-T4, and in healthy women. CIMT and endothelial function (using brachial artery ultrasound) were studied before and after L-T4 in a subgroup of women with SCH. RESULTS: Systolic and diastolic BP, total cholesterol, triglyceride, LDL-cholesterol, lipoprotein(a) and homocysteine were greater in SCH (P < 0.05), and following L-T4 replacement decreased (P < 0.05) to levels that no longer differed from normal subjects. Estimated GFR was reduced and serum Cystatin-C increased (P < 0.05) in SCH. These variables also normalized following L-T4. Following L-T4 replacement the carotid artery baseline diameter increased by 7.1% and CIMT decreased by a mean value of 13%, while brachial artery diameter increased basally by 12.5% and following endothelium-dependent vasodilatation by 17.5% (P < 0.05). However, the increment following reactive hyperaemia did not differ before or following L-T4 replacement. CONCLUSION: Normalization of

Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis

International Nuclear Information System (INIS)

Kibe, A.; Shimada, K.; Tagaki, Y.

1979-01-01

Hybrid ColE1 plasmids called ColE1-cos lambda-guaA or ColE1-cos lambda-gal can be efficiently transduced into various E.coli K-12 cells through packaging into lambda phage particles. Using these plasmids, repair of ultraviolet-light (UV) damaged ColE1 DNAs was studied in various UV sensitive E.coli K-12 mutants. The host mutations uvrA and uvrB markedly reduced host-cell reactivation of UV-irradiated ColE1-cos lambda-guaA. Pre-existing hybrid ColE1 plasmids had no effect on the frequency of lambda phage-mediated transduction of another differentially marked hybrid ColE1 DNAs. ColE1-cos lambda-guaA and ColE1-cos lambda-gal DNAs could temporarily but not stably co-exist in E.coli K-12 recA cells. The presence of ColE1-cos lambda-gal in uvrB cells promoted the repair of super-infected UV-irradiated ColE1-cos lambda-guaA about 7-fold. The same ColE1-cos lambda-gal plasmid in a uvrB recA double mutant did not have this promoting effect. These results indicate that the effect of resident hybrid ColE1 plasmids is manifested by the host recA + gene function(s) and suggest that ColE1 plasmit itself provides no recA + -like functions. (orig.) [de

Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis

Energy Technology Data Exchange (ETDEWEB)

Kibe, A; Shimada, K; Tagaki, Y [Kyushu Univ., Fukuoka (Japan). Dept. of Biochemistry

1979-01-01

Hybrid ColE1 plasmids called ColE1-cos lambda-guaA or ColE1-cos lambda-gal can be efficiently transduced into various E.coli K-12 cells through packaging into lambda phage particles. Using these plasmids, repair of ultraviolet-light (UV) damaged ColE1 DNAs was studied in various UV sensitive E.coli K-12 mutants. The host mutations uvrA and uvrB markedly reduced host-cell reactivation of UV-irradiated ColE1-cos lambda-guaA. Pre-existing hybrid ColE1 plasmids had no effect on the frequency of lambda phage-mediated transduction of another differentially marked hybrid ColE1 DNAs. ColE1-cos lambda-guaA and ColE1-cos lambda-gal DNAs could temporarily but not stably co-exist in E.coli K-12 recA cells. The presence of ColE1-cos lambda-gal in uvrB cells promoted the repair of super-infected UV-irradiated ColE1-cos lambda-guaA about 7-fold. The same ColE1-cos lambda-gal plasmid in a uvrB recA double mutant did not have this promoting effect. These results indicate that the effect of resident hybrid ColE1 plasmids is manifested by the host recA/sup +/ gene function(s) and suggest that ColE1 plasmit itself provides no recA/sup +/-like functions.

USAF Logistics Process Optimization Study for the Aircraft Asset Sustainment Process. Volume 2

National Research Council Canada - National Science Library

Adamson, Anthony

1998-01-01

.... It is published as three separate volumes. Volume I, USAF Logistics Process Optimization Study for the Aircraft Asset Sustainment Process -- Phase II Report, discusses the result and cost/benefit analysis of testing three initiatives...

USAF Logistics Process Optimization Study for the Aircraft Asset Sustainment Process. Volume 1

National Research Council Canada - National Science Library

Adamson, Anthony

1998-01-01

.... It is published as three separate volumes. Volume I, USAF Logistics Process Optimization Study for the Aircraft Asset Sustainment Process -- Phase II Report, discusses the result and cost/benefit analysis of testing three initiatives...

2012 ETA Guidelines: The Use of L-T4 + L-T3 in the Treatment of Hypothyroidism

DEFF Research Database (Denmark)

Nygaard, Birte

2012-01-01

. Results: Suggested explanations for persisting symptoms include: awareness of a chronic disease, presence of associated autoimmune diseases, thyroid autoimmunity per se, and inadequacy of L-T4 treatment to restore physiological thyroxine (T4) and triiodothyronine (T3) concentrations in serum and tissues...

Genetic influences on ovulation of primary oocytes in LT/Sv strain mice.

Science.gov (United States)

Everett, Clare A; Auchincloss, Catherine A; Kaufman, Matthew H; Abbott, Catherine M; West, John D

2004-11-01

A high proportion of LT/Sv strain oocytes arrest in meiotic metaphase I (MI) and are ovulated as diploid primary oocytes rather than haploid secondary oocytes. (Mus musculus castaneus x LT/SvKau)F1 x LT/SvKau backcross females were analysed for the proportion of oocytes that arrested in MI and typed by PCR for a panel of microsatellite DNA sequences (simple sequence repeat polymorphisms) that differed between strain LT/SvKau and M. m. castaneus. This provided a whole genome scan of 86 genetic markers distributed over all 19 autosomes and the X chromosome, and revealed genetic linkage of the MI arrest phenotype to markers on chromosomes 1 and 9. Identification of these two chromosomal regions should facilitate the identification of genes involved in mammalian oocyte maturation and the control of meiosis.

The transcription factor Lc-Maf participates in Col27a1 regulation during chondrocyte maturation

Energy Technology Data Exchange (ETDEWEB)

Mayo, Jaime L.; Holden, Devin N. [Department of Microbiology and Molecular Biology, Brigham Young University, 591 WIDB, Provo, UT 84602 (United States); Barrow, Jeffery R. [Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT 84602 (United States); Bridgewater, Laura C., E-mail: laura_bridgewater@byu.edu [Department of Microbiology and Molecular Biology, Brigham Young University, 591 WIDB, Provo, UT 84602 (United States)

2009-08-01

The transcription factor Lc-Maf, which is a splice variant of c-Maf, is expressed in cartilage undergoing endochondral ossification and participates in the regulation of type II collagen through a cartilage-specific Col2a1 enhancer element. Type XXVII and type XI collagens are also expressed in cartilage during endochondral ossification, and so enhancer/reporter assays were used to determine whether Lc-Maf could regulate cartilage-specific enhancers from the Col27a1 and Col11a2 genes. The Col27a1 enhancer was upregulated over 4-fold by Lc-Maf, while the Col11a2 enhancer was downregulated slightly. To confirm the results of these reporter assays, rat chondrosarcoma (RCS) cells were transiently transfected with an Lc-Maf expression plasmid, and quantitative RT-PCR was performed to measure the expression of endogenous Col27a1 and Col11a2 genes. Endogenous Col27a1 was upregulated 6-fold by Lc-Maf overexpression, while endogenous Col11a2 was unchanged. Finally, in situ hybridization and immunohistochemistry were performed in the radius and ulna of embryonic day 17 mouse forelimbs undergoing endochondral ossification. Results demonstrated that Lc-Maf and Col27a1 mRNAs are coexpressed in proliferating and prehypertrophic regions, as would be predicted if Lc-Maf regulates Col27a1 expression. Type XXVII collagen protein was also most abundant in prehypertrophic and proliferating chondrocytes. Others have shown that mice that are null for Lc-Maf and c-Maf have expanded hypertrophic regions with reduced ossification and delayed vascularization. Separate studies have indicated that Col27a1 may serve as a scaffold for ossification and vascularization. The work presented here suggests that Lc-Maf may affect the process of endochondral ossification by participating in the regulation of Col27a1 expression.

The ColM Family, Polymorphic Toxins Breaching the Bacterial Cell Wall

Directory of Open Access Journals (Sweden)

Maarten G. K. Ghequire

2018-02-01

Full Text Available Bacteria host an arsenal of antagonism-mediating molecules to combat for ecologic space. Bacteriocins represent a pivotal group of secreted antibacterial peptides and proteins assisting in this fight, mainly eliminating relatives. Colicin M, a model for peptidoglycan-interfering bacteriocins in Gram-negative bacteria, appears to be part of a set of polymorphic toxins equipped with such a catalytic domain (ColM targeting lipid II. Diversifying recombination has enabled parasitism of different receptors and has also given rise to hybrid bacteriocins in which ColM is associated with another toxin module. Remarkably, ColM toxins have recruited a diverse array of immunity partners, comprising cytoplasmic membrane-associated proteins with different topologies. Together, these findings suggest that different immunity mechanisms have evolved for ColM, in contrast to bacteriocins with nuclease activities.

A SEARCH FOR L/T TRANSITION DWARFS WITH PAN-STARRS1 AND WISE. II. L/T TRANSITION ATMOSPHERES AND YOUNG DISCOVERIES

International Nuclear Information System (INIS)

Best, William M. J.; Liu, Michael C.; Magnier, Eugene A.; Aller, Kimberly M.; Chambers, K. C.; Flewelling, H.; Hodapp, K. W.; Kaiser, N.; Tonry, J. L.; Wainscoat, R. J.; Waters, C.; Deacon, Niall R.; Redstone, Joshua; Burgett, W. S.; Draper, P.; Metcalfe, N.

2015-01-01

The evolution of brown dwarfs from L to T spectral types is one of the least understood aspects of the ultracool population, partly for lack of a large, well-defined, and well-characterized sample in the L/T transition. To improve the existing census, we have searched ≈28,000 deg 2 using the Pan-STARRS1 and Wide-field Infrared Survey Explorer surveys for L/T transition dwarfs within 25 pc. We present 130 ultracool dwarf discoveries with estimated distances ≈9–130 pc, including 21 that were independently discovered by other authors and 3 that were previously identified as photometric candidates. Seventy-nine of our objects have near-IR spectral types of L6–T4.5, the most L/T transition dwarfs from any search to date, and we have increased the census of L9–T1.5 objects within 25 pc by over 50%. The color distribution of our discoveries provides further evidence for the “L/T gap,” a deficit of objects with (J − K) MKO  ≈ 0.0–0.5 mag in the L/T transition, and thus reinforces the idea that the transition from cloudy to clear photospheres occurs rapidly. Among our discoveries are 31 candidate binaries based on their low-resolution spectral features. Two of these candidates are common proper motion companions to nearby main sequence stars; if confirmed as binaries, these would be rare benchmark systems with the potential to stringently test ultracool evolutionary models. Our search also serendipitously identified 23 late-M and L dwarfs with spectroscopic signs of low gravity implying youth, including 10 with vl-g or int-g gravity classifications and another 13 with indications of low gravity whose spectral types or modest spectral signal-to-noise ratio do not allow us to assign formal classifications. Finally, we identify 10 candidate members of nearby young moving groups (YMG) with spectral types L7–T4.5, including three showing spectroscopic signs of low gravity. If confirmed, any of these would be among the coolest known YMG members and would

Linking Acquisition Decisionmaking With National Military Strategy

Science.gov (United States)

1990-10-01

oil ; to defend vital US interests in the Pacific; and to respond to...milestone. The discussion of these issues is documented in a System Concept Paper ( SCP ) which includes a description of the selected alternative to...Wishbone Terrace Cabin John, MD. 20818 LtGen. George Sylvester, USAF (Ret.) 1 126 Takoma Lane Palm Beach Shores, FL. 33404 3 General William Y. Smith, USAF (Ret.) 6541 Brooks Place Falls Church, VA. 22044 3 I - I I I I I I DL,-I

Mastering Autodesk Inventor 2015 and Autodesk Inventor LT 2015

CERN Document Server

Waguespack, Curtis

2014-01-01

A comprehensive guide to Autodesk Inventor and Inventor LT This detailed reference and tutorial provides straightforward explanations, real-world examples, and practical tutorials that focus squarely on teaching Autodesk Inventor tips, tricks, and techniques. The book also includes a project at the beginning to help those new to Inventor quickly understand key interface conventions and capabilities. In addition, there is more information on Inventor LT, new practice drawings at the end of each chapter to reinforce lessons learned, and thorough coverage of all of Inventor's new features. The au

Colágeno na cartilagem osteoartrótica

Directory of Open Access Journals (Sweden)

Ana Paula P. Velosa

2003-06-01

Full Text Available A cartilagem articular é um tecido altamente especializado, composto por células, os condrócitos, e um conjunto de macromoléculas, como o colágeno e os proteoglicanos. O colágeno é uma proteína fibrilar que garante resistência ao tecido, enquanto os proteoglicanos têm a função de mola biológica, sendo responsáveis pela compressibilidade da cartilagem. A complexa interação entre estas duas proteínas garante a elasticidade. Estas características específicas da cartilagem são essenciais para amortecer as grandes forças de impacto a que as articulações diartrodiais estão submetidas, sem muito gasto de energia, visto tratar-se de um tecido avascular. Em processos artrósicos ocorre um desequilíbrio entre a produção de componentes da matriz extracelular e destruição pelas metaloproteases, levando à degradação e perda do tecido cartilaginoso. A fase inicial da osteoartrose é marcada por perda de fragmentos de proteoglicanos para o líquido sinovial, aumento dos colágenos tipo II e tipo VI, aparecimento dos colágenos I e III, não típicos da cartilagem, e diminuição do colágeno tipo IX, que é importante para manter a integridade da matriz extracelular, além do entumescimento da cartilagem. Como conseqüência, a cartilagem perde suas características específicas, levando a alterações na função articular. A evolução da doença promove diminuição significativa das proteínas, até mesmo do colágeno tipo XI, que tem localização mais interna na estrutura da fibrila heterotípica, e, portanto levando até a exposição do osso. Até o momento, o tratamento da osteoartrose está baseado principalmente no controle da dor e/ou inflamação, não diminuindo ou impedindo a degradação da cartilagem articular. Neste aspecto a perspectiva de tratamento futuro da osteoartrose estaria na utilização de inibidores das metaloproteases associadas a condroprotetores interferindo no "turnover" da cartilagem e

Tools for the Conceptual Design and Engineering Analysis of Micro Air Vehicles

Science.gov (United States)

2009-03-01

Approved: Dr. Robert A. Canfield Thesis Co-Advisor Date Lt.Col. Frederick G. Harmon Thesis Co-Advisor Date Lt.Col. Christopher Shearer Committee...this journey would have been felt in much greater amount. I would like to extend my sincere gratitude to Dr. Robert A. Canfield who provided an...rc=root_chord; tc=tip_chord; tr=taper_ratio; datcom_sa; cbar=2/3*rc*(1+tr+tr^2)/(1+tr) %mean aerodynamic chord Raymer p.56 Ybar=b/6*((1+2*tr)/(1+tr

The Egyptian Military Elite: An Operational Code

Science.gov (United States)

1977-09-01

17 Jan 1955. 6. Salem, Major Salah, "Mehalla’s Great Welcome For Major Salem," Egyptian Gazette, p. 1, 7 Jan 1955. 7. Muhieldin, Lt. Col. Zakaria, "The...Wheel of Reform Will Not Turn Back," Egyptian Gazette, p. 1, 26 Jan 1955. 8. Gohar, Lt. Col. Salah, "Imaginary Incident Explored," Egyptian Gazette...Says Army is Fully Prepared," Al Akhbar , FBIS Vol. 172, p. Al, 4 Sept 1956. 12. Sadat, Anwar al, "Western Powers Determined to Grab Canal," Al

Abortive phage-infection and UV-protection markers on ColI plasmids from epidemic strains of Salmonella

International Nuclear Information System (INIS)

Barker, R.M.

1988-01-01

Cultures of Escherichia coli carrying ColI plasmids received in conjugation from strains of Salmonella typhimurium and S. agona were examined for abortive infection (Abi) of phage BF23 and for enhanced resistance to the lethal action of UV-irradiation (Uvr). The Abi character of stored cultures of E. coli was also compared with the reaction of the same stock culture tested 5 years before. Seven of the eight potential types differentiated by three characters were represented among 160 ColI plasmids: ColIa Abi + Uvr + (3 plasmids), ColIa Abi - Uvr + (1), ColIa Abi - Uvr-> (2), ColIb Abi + Uvr + (85), ColIb Abi + Uvr - (5), ColIb Abi - Uvr + (4), ColIb Abi-? Uvr - (60). Recognition that different plasmid types could be carried by strains of a clone proved useful in the interpretation of the epidemic spread of strains of S. typhimurium of phage type/biotype 141/9f in Scotland and in tracing the ancestry of a recently emerged rhamnose non-fermenting mutant strain of S. agona. (author)

Comparison Results on the Basic Phenomena between CAP and CONTEMPT-LT

Energy Technology Data Exchange (ETDEWEB)

Choo, Yeon Joon; Hong, Soon Joon; Hwang, Su Hyun; Kim, Min Ki; Lee, Byung Chul [FNC Tech., SNU, Seoul (Korea, Republic of); Ha, Sang Jun; Choi, Hoon [KHNP Central Research Institute, Daejeon (Korea, Republic of)

2011-10-15

A development project for the domestic design code was launched to be used for the safety and performance analysis of pressurized light water reactors. As a part of this project, CAP (Containment Analysis Package) code has been developing for the containment safety and performance analysis side by side with SPACE. CAP Beta version has been released lately and validation processes are under way currently. Code by code comparison activity is scheduled in the validation processes and the first comparable code is CONTEMPT-LT. CONTEMPT-LT was developed to predict the longterm behavior of water-cooled nuclear reactor containment systems subjected to postulated loss-ofcoolant accident (LOCA) conditions. CONTEMPT-LT calculates the time variation of compartment pressures, temperatures, mass and energy inventories, heat structure temperature distributions, and energy exchange with adjacent compartments, leakage on containment response. Models are provided for fan cooler and cooling spray as engineered safety systems. Any compartment may have both a liquid pool region and an air-vapor atmosphere region above the pool. Each region is assumed to have a uniform temperature, but the temperatures of the two regions may be different. As mentioned above, CONTEMP-LT has the similar code features and it, therefore, is expected to show the similar analysis performance with CAP. In this study, the code performances were compared for the same phenomena between CAP and CONTEMPT-LT. Code comparison is carried out through two stages; separate and integral effect comparison

Comparison Results on the Basic Phenomena between CAP and CONTEMPT-LT

International Nuclear Information System (INIS)

Choo, Yeon Joon; Hong, Soon Joon; Hwang, Su Hyun; Kim, Min Ki; Lee, Byung Chul; Ha, Sang Jun; Choi, Hoon

2011-01-01

A development project for the domestic design code was launched to be used for the safety and performance analysis of pressurized light water reactors. As a part of this project, CAP (Containment Analysis Package) code has been developing for the containment safety and performance analysis side by side with SPACE. CAP Beta version has been released lately and validation processes are under way currently. Code by code comparison activity is scheduled in the validation processes and the first comparable code is CONTEMPT-LT. CONTEMPT-LT was developed to predict the longterm behavior of water-cooled nuclear reactor containment systems subjected to postulated loss-ofcoolant accident (LOCA) conditions. CONTEMPT-LT calculates the time variation of compartment pressures, temperatures, mass and energy inventories, heat structure temperature distributions, and energy exchange with adjacent compartments, leakage on containment response. Models are provided for fan cooler and cooling spray as engineered safety systems. Any compartment may have both a liquid pool region and an air-vapor atmosphere region above the pool. Each region is assumed to have a uniform temperature, but the temperatures of the two regions may be different. As mentioned above, CONTEMP-LT has the similar code features and it, therefore, is expected to show the similar analysis performance with CAP. In this study, the code performances were compared for the same phenomena between CAP and CONTEMPT-LT. Code comparison is carried out through two stages; separate and integral effect comparison

COL11A1 in FAP polyps and in sporadic colorectal tumors

International Nuclear Information System (INIS)

Fischer, Heléne; Salahshor, Sima; Stenling, Roger; Björk, Jan; Lindmark, Gudrun; Iselius, Lennart; Rubio, Carlos; Lindblom, Annika

2001-01-01

We previously reported that the α-1 chain of type 11 collagen (COL11A1), not normally expressed in the colon, was up-regulated in stromal fibroblasts in most sporadic colorectal carcinomas. Patients with germline mutations in the APC gene show, besides colonic polyposis, symptoms of stromal fibroblast involvement, which could be related to COL11A1 expression. Most colorectal carcinomas are suggested to be a result of an activated Wnt- pathway, most often involving an inactivation of the APC gene or activation of β-catenin. We used normal and polyp tissue samples from one FAP patient and a set of 37 sporadic colorectal carcinomas to find out if the up-regulation of COL11A1 was associated with an active APC/β-catenin pathway. In this study we found a statistically significant difference in COL11A1 expression between normal tissue and adenomas from one FAP patient, and all adenomas gave evidence for an active APC/β-catenin pathway. An active Wnt pathway has been suggested to involve stromal expression of WISP-1. We found a strong correlation between WISP-1 and COL11A1 expression in sporadic carcinomas. Our results suggest that expression of COL11A1 in colorectal tumors could be associated with the APC/β-catenin pathway in FAP and sporadic colorectal cancer

CysLT(1)R antagonists inhibit tumor growth in a xenograft model of colon cancer.

Science.gov (United States)

Savari, Sayeh; Liu, Minghui; Zhang, Yuan; Sime, Wondossen; Sjölander, Anita

2013-01-01

The expression of the inflammatory G-protein coupled receptor CysLT1R has been shown to be upregulated in colon cancer patients and associated with poor prognosis. The present study investigated the correlation between CysLT1R and colon cancer development in vivo using CysLT1R antagonists (ZM198,615 or Montelukast) and the nude mouse xenograft model. Two drug administration regimens were established. The first regimen was established to investigate the importance of CysLT1R in tumor initiation. Nude mice were inoculated with 50 µM CysLT1R antagonist-pretreated HCT-116 colon cancer cells and received continued treatment (5 mg/kg/day, intraperitoneally). The second regimen aimed to address the role of CysLT1R in tumor progression. Nude mice were inoculated with non-pretreated HCT-116 cells and did not receive CysLT1R antagonist treatment until recordable tumor appearance. Both regimens resulted in significantly reduced tumor size, attributed to changes in proliferation and apoptosis as determined by reduced Ki-67 levels and increased levels of p21(WAF/Cip1) (Pcolon cancer cell line HCT-116 and CysLT1R antagonists. In addition to significant reductions in cell proliferation, adhesion and colony formation, we observed induction of cell cycle arrest and apoptosis in a dose-dependent manner. The ability of Montelukast to inhibit growth of human colon cancer xenograft was further validated by using two additional colon cancer cell lines, SW-480 and HT-29. Our results demonstrate that CysLT1R antagonists inhibit growth of colon cancer xenografts primarily by reducing proliferation and inducing apoptosis of the tumor cells.

Gaming the System: A Game Theory Analysis of Theater Airborne ISR

Science.gov (United States)

2014-02-13

Brown , Jason M., Col, USAF. "Strategy for Intelligence, Surveillance, and Reconnaissance." AWC Research Report, Air University, 2013. Builder, Carl H...Nathan, MAJ, USA (division collection manager in Regional Command – South, 2012- 2013), Interview by Author. September 2013. Morgan, Gareth . Images of

Defense AT and L. Volume 37, Number 6

Science.gov (United States)

2008-12-01

Uncertainty, and Trouble Escaping the RUT of Program Instability Col. Brian Shimel, USAF “However beautiful the strategy, you should occasionally...fielding of cutting-edge equipment in a teleconference with bloggers and online journalists. “This decision reflects the need to move more aggres- sively

Scoliosis in osteogenesis imperfecta caused by COL1A1/COL1A2 mutations - genotype-phenotype correlations and effect of bisphosphonate treatment.

Science.gov (United States)

Sato, Atsuko; Ouellet, Jean; Muneta, Takeshi; Glorieux, Francis H; Rauch, Frank

2016-05-01

Bisphosphonates are widely used to treat children with osteogenesis imperfecta (OI), a bone fragility disorder that is most often caused by mutations in COL1A1 or COL1A2. However, it is unclear whether this treatment decreases the risk of developing scoliosis. We retrospectively evaluated spine radiographs and charts of 437 patients (227 female) with OI caused by mutations in COL1A1 or COL1A2 and compared the relationship between scoliosis, genotype and bisphosphonate treatment history. At the last follow-up (mean age 11.9 [SD: 5.9] years), 242 (55%) patients had scoliosis. The prevalence of scoliosis was highest in OI type III (89%), followed by OI type IV (61%) and OI type I (36%). Moderate to severe scoliosis (Cobb angle ≥25°) was rare in individuals with COL1A1 haploinsufficiency mutations but was present in about two fifth of patients with triple helical glycine substitutions or C-propeptide mutations. During the first 2 to 4years of bisphosphonate therapy, patients with OI type III had lower Cobb angle progression rates than before bisphosphonate treatment, whereas in OI types I and IV bisphosphonate treatment was not associated with a change in Cobb angle progression rates. At skeletal maturity, the prevalence of scoliosis (Cobb angle >10°) was similar in patients who had started bisphosphonate treatment early in life (before 5.0years of age) and in patients who had started therapy later (after the age of 10.0years) or had never received bisphosphonate therapy. Bisphosphonate treatment decreased progression rate of scoliosis in OI type III but there was no evidence of a positive effect on scoliosis in OI types I and IV. The prevalence of scoliosis at maturity was not influenced by the bisphosphonate treatment history in any OI type. Copyright © 2016 Elsevier Inc. All rights reserved.

Simulation-Based e-Learning Tools for Science,Engineering, and Technology Education(SimBeLT)

Science.gov (United States)

Davis, Doyle V.; Cherner, Y.

2006-12-01

The focus of Project SimBeLT is the research, development, testing, and dissemination of a new type of simulation-based integrated e-learning set of modules for two-year college technical and engineering curricula in the areas of thermodynamics, fluid physics, and fiber optics that can also be used in secondary schools and four-year colleges. A collection of sophisticated virtual labs is the core component of the SimBeLT modules. These labs will be designed to enhance the understanding of technical concepts and underlying fundamental principles of these topics, as well as to master certain performance based skills online. SimBeLT software will help educators to meet the National Science Education Standard that "learning science and technology is something that students do, not something that is done to them". A major component of Project SimBeLT is the development of multi-layered technology-oriented virtual labs that realistically mimic workplace-like environments. Dynamic data exchange between simulations will be implemented and links with instant instructional messages and data handling tools will be realized. A second important goal of Project SimBeLT labs is to bridge technical skills and scientific knowledge by enhancing the teaching and learning of specific scientific or engineering subjects. SimBeLT builds upon research and outcomes of interactive teaching strategies and tools developed through prior NSF funding (http://webphysics.nhctc.edu/compact/index.html) (Project SimBeLT is partially supported by a grant from the National Science Foundation DUE-0603277)

AutoCAD 2015 and AutoCAD LT 2015 bible

CERN Document Server

Finkelstein, Ellen

2014-01-01

The perfect reference for all AutoCAD users AutoCAD 2015 and AutoCAD LT 2015 Bible is the book you want to have close at hand to answer those day-to-day questions about this industry-leading software. Author and Autodesk University instructor Ellen Finkelstein guides readers through AutoCAD 2015 and AutoCAD LT 2015 with clear, easy-to-understand instruction and hands-on tutorials that allow even total beginners to create a design on their very first day. Although simple and fundamental enough to be used by those new to CAD, the book is so comprehensive that even Autodesk power u

Purposeful Development of the Intelligence, Surveillance, and Reconnaissance for Space Cadre

Science.gov (United States)

2016-07-13

This article would not have been possible without the invaluable thoughts and perspectives provided by Col Gay “Charlie” McGillis, USAF, retired, and...should move on to a Twenty-Fifth Air Force or intelligence community assignment that allows them to fulfill ISR requirements that, when answered

Entomopathogenic fungi in predatory beetles (Col: Carabidae and Staphylinidae) from agricultural fields

DEFF Research Database (Denmark)

Steenberg, T; Langer, V; Esbjerg, P

1995-01-01

Prevalence of entomopathogenic fungi was studied in overwintering ground beetles (Col.: Carabidae) and rove beetles (Col.: Staphylinidae) collected from fields of lucerne, white cabbage and white cabbage undersown with white clover. In general infection levels in adult ground beetles and rove bee...

UEP LT Codes with Intermediate Feedback

DEFF Research Database (Denmark)

Sørensen, Jesper Hemming; Popovski, Petar; Østergaard, Jan

2013-01-01

We analyze a class of rateless codes, called Luby transform (LT) codes with unequal error protection (UEP). We show that while these codes successfully provide UEP, there is a significant price in terms of redundancy in the lower prioritized segments. We propose a modification with a single inter...... intermediate feedback message. Our analysis shows a dramatic improvement on the decoding performance of the lower prioritized segment....

USAF Summer Research Program - 1995 High School Apprenticeship Program Final Reports, Volume 14, Rome Laboratory

National Research Council Canada - National Science Library

Moore, Gary

1995-01-01

The United States Air Force High School Apprenticeship Program's (USAF HSAP) purpose is to place outstanding high school students whose interests are in the areas of mathematics, engineering, and science to work in a laboratory environment...

USAF TPS L-23 Shear Wind Observed Optimized Path Investigation for NASA (SENIOR ShWOOPIN)

National Research Council Canada - National Science Library

Gordon, Randy; Fails, Robert; Baase, Solomon; Eckberg, Jason; Ryan, Charles; Smith, Chris

2006-01-01

The SENIOR ShWOOPIN TMP was conducted at the request of the USAF TPS as part of a NASA investigation into the viability of aircraft endurance enhancement through the extraction of energy from horizontal wind gradients...

Evaluation the COL9A2 gene with high myopia

Science.gov (United States)

Zhang, Dingding; Huang, Maomin

2017-11-01

This paper investigates the association of the COL9A2 gene between high myopia and normal controls in the Han Chinese population. It shows that the frameshift mutation (D281fs) in the COL9A2 gene is not associated with high myopia in the Han Chinese population, and the two novel variants(c.143G＞C and c.884G＞A) may contribute to the development of high myopia.

Air & Space Power Journal (ASPJ). Volume 26, Number 6

Science.gov (United States)

2012-12-01

and Reconnaissance Col Matthew M. Hurley, USAF Thirty years ago, at the dawn of the digital age, the notion of a synthetic , virtual realm where human...amphetamines, a refined, high-value drug made domestically and having advantages over both marijuana (which requires space to grow and whose bulk

Low temperature solid oxide electrolytes (LT-SOE): A review

Science.gov (United States)

Singh, B.; Ghosh, S.; Aich, S.; Roy, B.

2017-01-01

Low temperature solid oxide fuel cell (LT-SOFC) can be a source of power for vehicles, online grid, and at the same time reduce system cost, offer high reliability, and fast start-up. A huge amount of research work, as evident from the literature has been conducted for the enhancement of the ionic conductivity of LT electrolytes in the last few years. The basic conduction mechanisms, advantages and disadvantages of different LT oxide ion conducting electrolytes {BIMEVOX systems, bilayer systems including doped cerium oxide/stabilised bismuth oxide and YSZ/DCO}, mixed ion conducting electrolytes {doped cerium oxides/alkali metal carbonate composites}, and proton conducting electrolytes {doped and undoped BaCeO3, BaZrO3, etc.} are discussed here based on the recent research articles. Effect of various material aspects (composition, doping, layer thickness, etc.), fabrication methods (to achieve different microstructures and particle size), design related strategies (interlayer, sintering aid etc.), characterization temperature & environment on the conductivity of the electrolytes and performance of the fuel cells made from these electrolytes are shown in tabular form and discussed. The conductivity of the electrolytes and performance of the corresponding fuel cells are compared. Other applications of the electrolytes are mentioned. A few considerations regarding the future prospects are pointed.

Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNA

Science.gov (United States)

Hershfield, Vickers; Boyer, Herbert W.; Yanofsky, Charles; Lovett, Michael A.; Helinski, Donald R.

1974-01-01

DNA fragments obtained from EcoRI endonuclease digestion of bacteriophage ϕ80pt190 (trp+) and the plasmid ColE1 were covalently joined with polynucleotide ligase. Transformation of Escherichia coli trp- strains to tryptophan independence with the recombined DNA selected for reconstituted ColE1 plasmids containing the tryptophan operon and the ϕ80 immunity region. Similarly, an EcoRI endonuclease generated fragment of plasmid pSC105 DNA containing the genetic determinant of kanamycin resistance was inserted into the ColE1 plasmid and recovered in E. coli. The plasmids containing the trp operon (ColE1-trp) and the kanamycin resistance gene were maintained under logarithmic growth conditions at a level of 25-30 copies per cell and accumulate to the extent of several hundred copies per cell in the presence of chloramphenicol. Cells carrying the ColE1-trp plasmid determined the production of highly elevated levels of trp operon-specific mRNA and tryptophan biosynthetic enzymes. Images PMID:4610576

The Saccharomyces boulardii CNCM I-745 Strain Shows Protective Effects against the B. anthracis LT Toxin

Directory of Open Access Journals (Sweden)

Rodolphe Pontier-Bres

2015-10-01

Full Text Available The probiotic yeast Saccharomyces boulardii (S. boulardii has been prescribed for the prophylaxis and treatment of several infectious diarrheal diseases. Gastrointestinal anthrax causes fatal systemic disease. In the present study, we investigated the protective effects conferred by Saccharomyces boulardii CNCM I-745 strain on polarized T84 columnar epithelial cells intoxicated by the lethal toxin (LT of Bacillus anthracis. Exposure of polarized T84 cells to LT affected cell monolayer integrity, modified the morphology of tight junctions and induced the formation of actin stress fibers. Overnight treatment of cells with S. boulardii before incubation with LT maintained the integrity of the monolayers, prevented morphological modification of tight junctions, restricted the effects of LT on actin remodeling and delayed LT-induced MEK-2 cleavage. Mechanistically, we demonstrated that in the presence of S. boulardii, the medium is depleted of both LF and PA sub-units of LT and the appearance of a cleaved form of PA. Our study highlights the potential of the S. boulardii CNCM I-745 strain as a prophylactic agent against the gastrointestinal form of anthrax.

The Saccharomyces boulardii CNCM I-745 strain shows protective effects against the B. anthracis LT toxin.

Science.gov (United States)

Pontier-Bres, Rodolphe; Rampal, Patrick; Peyron, Jean-François; Munro, Patrick; Lemichez, Emmanuel; Czerucka, Dorota

2015-10-30

The probiotic yeast Saccharomyces boulardii (S. boulardii) has been prescribed for the prophylaxis and treatment of several infectious diarrheal diseases. Gastrointestinal anthrax causes fatal systemic disease. In the present study, we investigated the protective effects conferred by Saccharomyces boulardii CNCM I-745 strain on polarized T84 columnar epithelial cells intoxicated by the lethal toxin (LT) of Bacillus anthracis. Exposure of polarized T84 cells to LT affected cell monolayer integrity, modified the morphology of tight junctions and induced the formation of actin stress fibers. Overnight treatment of cells with S. boulardii before incubation with LT maintained the integrity of the monolayers, prevented morphological modification of tight junctions, restricted the effects of LT on actin remodeling and delayed LT-induced MEK-2 cleavage. Mechanistically, we demonstrated that in the presence of S. boulardii, the medium is depleted of both LF and PA sub-units of LT and the appearance of a cleaved form of PA. Our study highlights the potential of the S. boulardii CNCM I-745 strain as a prophylactic agent against the gastrointestinal form of anthrax.

Mixed Methods Approach to Identify Factors and the Extent to Which They Influence Medical/Surgical Prime Vendor Use

Science.gov (United States)

2014-03-27

would not have been as enjoyable. I would also like to thank Col (ret) Don Faust, Col Duane Bragg and Lt Col (ret) Chris Canales for being...on the effectiveness of an IS utility in an organization (Dong, Neufeld, Higgins , 2009). Evidence suggests that top managers must adjust their...Neufeld, Higgins , 2009) and to advancements in organizational performance (Russell, Terborg, and Powers, 1985; Bartel, 1994; Knoke and Kalleberg, 1994

CysLT2 receptor activation is involved in LTC4-induced lung air-trapping in guinea pigs.

Science.gov (United States)

Sekioka, Tomohiko; Kadode, Michiaki; Yonetomi, Yasuo; Kamiya, Akihiro; Fujita, Manabu; Nabe, Takeshi; Kawabata, Kazuhito

2017-01-05

CysLT 1 receptors are known to be involved in the pathogenesis of asthma. However, the functional roles of CysLT 2 receptors in this condition have not been determined. The purpose of this study is to develop an experimental model of CysLT 2 receptor-mediated LTC 4 -induced lung air-trapping in guinea pigs and use this model to clarify the mechanism underlying response to such trapping. Because LTC 4 is rapidly converted to LTD 4 by γ-glutamyltranspeptidase (γ-GTP) under physiological conditions, S-hexyl GSH was used as a γ-GTP inhibitor. In anesthetized artificially ventilated guinea pigs with no S-hexyl GSH treatment, i.v. LTC 4 -induced bronchoconstriction was almost completely inhibited by montelukast, a CysLT 1 receptor antagonist, but not by BayCysLT 2 RA, a CysLT 2 receptor antagonist. The inhibitory effect of montelukast was diminished by treatment with S-hexyl GSH, whereas the effect of BayCysLT 2 RA was enhanced with increasing dose of S-hexyl GSH. Macroscopic and histological examination of lung tissue isolated from LTC 4 -/S-hexyl-GSH-treated guinea pigs revealed air-trapping expansion, particularly at the alveolar site. Inhaled LTC 4 in conscious guinea pigs treated with S-hexyl GSH increased both airway resistance and airway hyperinflation. On the other hand, LTC 4 -induced air-trapping was only partially suppressed by treatment with the bronchodilator salmeterol. Although montelukast inhibition of LTC 4 -induced air-trapping was weak, treatment with BayCysLT 2 RA resulted in complete suppression of this air-trapping. Furthermore, BayCysLT 2 RA completely suppressed LTC 4 -induced airway vascular hyperpermeability. In conclusion, we found in this study that CysLT 2 receptors mediate LTC 4 -induced bronchoconstriction and air-trapping in S-hexyl GSH-treated guinea pigs. It is therefore believed that CysLT 2 receptors contribute to asthmatic response involving air-trapping. Copyright © 2016 Elsevier B.V. All rights reserved.

A role for inflammatory mediators in heterologous desensitization of CysLT1 receptor in human monocytes

Science.gov (United States)

Capra, Valérie; Accomazzo, Maria Rosa; Gardoni, Fabrizio; Barbieri, Silvia; Rovati, G. Enrico

2010-01-01

Cysteinyl-leukotrienes (cysteinyl-LT) are rapidly generated at sites of inflammation and, in addition to their role in asthma, rhinitis, and other immune disorders, are increasingly regarded as significant inflammatory factors in cancer, gastrointestinal, cardiovascular diseases. We recently demonstrated that in monocyte/macrophage–like U937 cells, extracellular nucleotides heterologously desensitize CysLT1 receptor (CysLT1R)-induced Ca2+ transients. Given that monocytes express a number of other inflammatory and chemoattractant receptors, this study was aimed at characterizing transregulation between these different stimuli. We demonstrate that in U937 cells and in primary human monocytes, a series of inflammatory mediators activating Gi-coupled receptor (FPR1, BLT1) desensitize CysLT1R-induced Ca2+ response unidirectionally through activation of PKC. Conversely, PAF-R, exclusively coupled to Gq, cross-desensitizes CysLT1R without the apparent involvement of any kinase. Interestingly, Gs-coupled receptors (β2AR, H1/2R, EP2/4R) are also able to desensitize CysLT1R response through activation of PKA. Heterologous desensitization seems to affect mostly the Gi-mediated signaling of the CysLT1R. The hierarchy of desensitization among agonists may be important for leukocyte signal processing at the site of inflammation. Considering that monocytes/macrophages are likely to be the major source of cysteinyl-LT in many immunological and inflammatory processes, shedding light on how their receptors are regulated will certainly help to better understand the role of these cells in orchestrating this complex network of integrated signals. PMID:19965602

Sharing the Burden and Risk: An Operational Assessment of the Reserve Components in Operation Iraqi Freedom

Science.gov (United States)

2016-10-01

Assessment, Joint Test and Evaluation, 1 June 2004. E-3 Commander Intent Met” field. Fields left blank or comments such as “UNK” and “ clouds obscured...Report of the Military Compensation and Retirement Modernization Commission: Final Report, 29 January 2015. Miller, Drew, Col USAFR, HQ USAF A8FX

TUMOR-SPECIFIC EXPRESSION AND ALTERNATIVE SPLICING OF THE COL6A3 GENE IN PANCREATIC CANCER

Science.gov (United States)

Arafat, Hwyda; Lazar, Melissa; Salem, Khalifa; Chipitsyna, Galina; Gong, Qiaoke; Pan, Te-Cheng; Zhang, Rui-Zhu; Yeo, Charles J.; Chu, Mon-Li

2011-01-01

Introduction Pancreatic ductal adenocarcinoma (PDA) is a highly lethal disease in which a prominent desmoplastic reaction is a defining characteristic. Fibrillar collagens, such as collagen I and to a lesser extent, collagen III and V comprise the majority of this stromal fibrosis. Type VI collagen (COL6) forms a microfibrillar network associated with type I collagen fibrils. The expression of COL6 has been linked to inflammation and survival. Importantly, tumor-specific alternative splicing in COL6A3 has been identified in several cancers by genome exon arrays. We evaluated the expression and localization of COL6A3 in PDA and premalignant lesions and explored the presence of alternative splicing events. Methods We analyzed paired PDA-normal (n=18), IPMN (n=5), pancreatic cystadenoma (n=5), and eight PDA cell lines with RT-PCR, using unique primers that identify total COL6A3 gene and alternative splicing sites in several of its exons. Western blot analysis and immunohistochemistry were used to analyze the expression levels and localization of COL6A3 protein in the different lesions, and in two animal models of PDA. Results COL6A3 protein levels were significantly upregulated in 77% of the paired PDA-adjacent tissue examined. COL6A3 was mainly present in the desmoplastic stroma of PDA, with high deposition around the malignant ducts and in between the sites of stromal fatty infiltration. Analysis of the COL6A3 splice variants showed tumor-specific consistent inclusion of exons 3 and 6 in 17 of the 18 (94%) paired PDA-adjacent tissues. Inclusion of exon 4 was exclusively tumor-specific, with barely detectable expression in the adjacent tissues. IPMN and pancreatic cystadenomas showed no expression of any of the examined exons. Total COL6A3 mRNA and exon 6 were identified in six PDA cell lines, but only two cell lines (MIA PACA-2 and ASPC-1) expressed exons 3 and 4. In both the xenograft and transgenic models of PDA, COL6A3 immunoreactivity was present in the stroma

A novel COL4A3 mutation causes autosomal-recessive Alport syndrome in a large Turkish family.

Science.gov (United States)

Uzak, Asli Subasioglu; Tokgoz, Bulent; Dundar, Munis; Tekin, Mustafa

2013-03-01

Alport syndrome (AS) is a genetically heterogeneous disorder that is characterized by hematuria, progressive renal failure typically resulting in end-stage renal disease, sensorineural hearing loss, and variable ocular abnormalities. Only 15% of cases with AS are autosomal recessive and are caused by mutations in the COL4A3 or COL4A4 genes, encoding type IV collagen. Clinical data in a large consanguineous family with four affected members were reviewed, and genomic DNA was extracted. For mapping, 15 microsatellite markers flanking COL4A3, COL4A4, and COL4A5 in 16 family members were typed. For mutation screening, all coding exons of COL4A3 were polymerase chain reaction- amplified and Sanger-sequenced from genomic DNA. The disease locus was mapped to chromosome 2q36.3, where COL4A3 and COL4A4 reside. Sanger sequencing revealed a novel mis-sense mutation (c.2T>C; p.M1T) in exon 1 of COL4A3. The identified nucleotide change was not found in 100 healthy ethnicity-matched controls via Sanger sequencing. We present a large consanguineous Turkish family with AS that was found to have a COL4A3 mutation as the cause of the disease. Although the relationship between the various genotypes and phenotypes in AS has not been fully elucidated, detailed clinical and molecular analyses are helpful for providing data to be used in genetic counseling. It is important to identify new mutations to clarify their clinical importance, to assess the prognosis of the disease, and to avoid renal biopsy for final diagnosis.

Advancements in the treatment of hypothyroidism with L-T4 liquid formulation or soft gel capsule: an update.

Science.gov (United States)

Fallahi, Poupak; Ferrari, Silvia Martina; Ruffilli, Ilaria; Ragusa, Francesca; Biricotti, Marco; Materazzi, Gabriele; Miccoli, Paolo; Antonelli, Alessandro

2017-05-01

The most recent advance concerning levothyroxine (L-T4) therapy is the development of novel oral formulations: the liquid preparation, and the soft gel capsule. Areas covered: This review evaluates the most recent clinical studies about these new formulations. The liquid formulation has been shown to overcome: the food and beverages intereference with L-T4 tablets absorption, caused by food or coffee at breakfast; malabsorption induced by the increased gastric pH, resulting from atrophic gastritis, or due to proton-pump inhibitors; and malabsorption after bariatric surgery. The use of liquid L-T4 has been studied also in pregnancy, newborns and infants, suggesting a better bioequivalence than tablets. Finally, liquid L-T4 is more active than tablets in the control of thyroid-stimulating hormone (TSH) in hypothyroid patients without malabsorption, drug interference, or gastric disorders, leading to a hypothesized higher absorption of liquid L-T4 also in these patients. Few studies have evaluated soft gel L-T4 with promising results in patients with malabsorption related to coffee or gastritis. Expert opinion: Liquid L-T4 (and soft gel capsules) are more active than the tablet L-T4 in the control of TSH in hypothyroid patients with gastric disorders, malabsorption, or drug interference, but also in patients without absorption disorders.

Mutations in COL1A1 and COL1A2 and dental aberrations in children and adolescents with osteogenesis imperfecta – A retrospective cohort study

Science.gov (United States)

Dahllöf, Göran; Lindahl, Katarina; Kindmark, Andreas; Grigelioniene, Giedre; Åström, Eva; Malmgren, Barbro

2017-01-01

Osteogenesis imperfecta (OI) is a heterogeneous group of disorders of connective tissue, caused mainly by mutations in the collagen I genes (COL1A1 and COL1A2). Dentinogenesis imperfecta (DGI) and other dental aberrations are common features of OI. We investigated the association between collagen I mutations and DGI, taurodontism, and retention of permanent second molars in a retrospective cohort of 152 unrelated children and adolescents with OI. The clinical examination included radiographic evaluations. Teeth from 81 individuals were available for histopathological evaluation. COL1A1/2 mutations were found in 104 individuals by nucleotide sequencing. DGI was diagnosed clinically and radiographically in 29% of the individuals (44/152) and through isolated histological findings in another 19% (29/152). In the individuals with a COL1A1 mutation, 70% (7/10) of those with a glycine substitution located C-terminal of p.Gly305 exhibited DGI in both dentitions while no individual (0/7) with a mutation N-terminal of this point exhibited DGI in either dentition (p = 0.01). In the individuals with a COL1A2 mutation, 80% (8/10) of those with a glycine substitution located C terminal of p.Gly211 exhibited DGI in both dentitions while no individual (0/5) with a mutation N-terminal of this point (p = 0.007) exhibited DGI in either dentition. DGI was restricted to the deciduous dentition in 20 individuals. Seventeen had missense mutations where glycine to serine was the most prevalent substitution (53%). Taurodontism occurred in 18% and retention of permanent second molars in 31% of the adolescents. Dental aberrations are strongly associated with qualitatively changed collagen I. The varying expressivity of DGI is related to the location of the collagen I mutation. Genotype information may be helpful in identifying individuals with OI who have an increased risk of dental aberrations. PMID:28498836

Persistence of Salmonella Typhimurium LT2 in Soil Enhanced after Growth in Lettuce Medium

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Kornelia Smalla

2017-04-01

Full Text Available The persistence of Salmonella in the environment is influenced by a multitude of biotic and abiotic factors. In addition, its persistence can be influenced by preadaptation before the introduction into the environment. In order to study how preadaptation changes the survival of Salmonella in soil and therefore its potential to colonize the phytosphere, we developed a new medium based on lettuce material [lettuce medium (LM]. Salmonella enterica serovar Typhimurium strain LT2 was used as a model for Salmonella in this study. LT2 was inoculated into soil microcosms after pregrowth in Luria Bertani (LB broth or in LM. Survival of LT2 in soil was monitored over 56 days by plate counts and quantification of the Typhimurium-specific gene STM4497 using qPCR in total community DNA for which primers and TaqMan probe were designed in this study. Significantly enhanced persistence was observed for LT2 pregrown in LM compared to LT2 pregrown in LB, indicating a preadaptation effect. Surprisingly, no improved survival could be observed for S. Typhimurium strain 14028s and S. enterica serovar Senftenberg after pregrowth on LM. This indicates a high strain specificity of preadaptation. Results from previous studies suggested that biofilm formation could enhance the survival of human pathogens in various environments and might contribute to enhanced survival on plants. In vitro biofilm assays with several Salmonella strains revealed a strain-specific effect of LM on the biofilm formation. While LM significantly improved the biofilm formation of S. Senftenberg, the biofilm formation of LT2 was better in LB. This indicates that the better survival of LM-pregrown LT2 in soil was not linked to an improved ability to form biofilms but was likely due to other factors. Most importantly, this study showed that the medium used to pregrow Salmonella can influence its survival in soil and its biofilm formation which might influence the fate of Salmonella in soil.

Persistent Notochord in a Fetus with COL2A1 Mutation

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Elisabeth Codsi

2015-01-01

Full Text Available Multiple anomalies including micromelia, poor mineralization of the vertebrae, and a persistent notochord were identified on second trimester ultrasound in a fetus with a COL2A1 mutation. To our knowledge, this represents the first case of a persistent notochord associated with a COL2A1 mutation in humans. In this case report, we describe ultrasound and postmortem findings and review the pathogenesis associated with a persistent notochord.

Overexpression of COL11A1 by cancer-associated fibroblasts: clinical relevance of a stromal marker in pancreatic cancer.

Directory of Open Access Journals (Sweden)

Carmen García-Pravia

Full Text Available BACKGROUND: The collagen11A1 (COL11A1 gene is overexpressed in pancreatic cancer. The expression of COL11A1 protein could be involved in desmoplastic events in pancreatic cancer, but an antibody that specifically stains the COL11A1 protein is not currently available. METHODS AND FINDINGS: A total of 54 pancreatic ductal adenocarcinomas (PDAC, 23 chronic pancreatitis (CP samples, and cultured peritumoral stromal cells of PDAC (passages 3-6 were studied. Normal human pancreas tissue samples were obtained through a cadaveric organ donation program. 1 Validation of COL11A1 gene overexpression by q-RT-PCR. FINDINGS: the expression of COL11A1 gene is significantly increased in PDAC samples vs. normal and CP samples. 2 Analysis of COL11A1 by immunohistochemistry using highly specific anti-proCOL11A1 antibodies. FINDINGS: anti-proCOL11A1 stains stromal cells/cancer-associated fibroblasts (CAFs of PDAC but it does not stain chronic benign condition (chronic pancreatitis stromal cells, epithelial cells, or normal fibroblasts. 3 Evaluation of the discrimination ability of the antibody. FINDINGS: anti-proCOL11A1 immunostaining accurately discriminates between PDAC and CP (AUC 0.936, 95% CI 0.851, 0.981. 4 Phenotypic characterization of proCOL11A1+ stromal cells co-staining with mesenchymal, epithelial and stellate cell markers on pancreatic tissue samples and cultured peritumoral pancreatic cancer stromal cells. FINDINGS: ProCOL11A1+ cells present co-staining with mesenchymal, stellate and epithelial markers (EMT phenotype in different proportions. CONCLUSIONS/SIGNIFICANCE: Detection of proCOL11A1 through immunostaining with this newly-developed antibody allows for a highly accurate distinction between PDAC and CP. Unlike other available antibodies commonly used to detect CAFs, anti-proCOL11A1 is negative in stromal cells of the normal pancreas and almost absent in benign inflammation. These results strongly suggest that proCOL11A1 is a specific marker for

Structural characterization of the Salmonella typhimurium LT2 umu operon

International Nuclear Information System (INIS)

Thomas, S.M.; Crowne, H.M.; Pidsley, S.C.; Sedgwick, S.G.

1990-01-01

The umuDC operon of Escherichia coli encodes functions required for mutagenesis induced by radiation and a wide variety of chemicals. The closely related organism Salmonella typhimurium is markedly less mutable than E. coli, but a umu homolog has recently been identified and cloned from the LT2 subline. In this study the nucleotide sequence and structure of the S. typhimurium LT2 umu operon have been determined and its gene products have been identified so that the molecular basis of umu activity might be understood more fully. S. typhimurium LT2 umu consists of a smaller 417-base-pair (bp) umuD gene ending 2 bp upstream of a larger 1,266-bp umuC gene. The only apparent structural difference between the two operons is the lack of gene overlap. An SOS box identical to that found in E. coli is present in the promoter region upstream of umuD. The calculated molecular masses of the umuD and umuC gene products were 15.3 and 47.8 kilodaltons, respectively, which agree with figures determined by transpositional disruption and maxicell analysis. The S. typhimurium and E. coli umuD sequences were 68% homologous and encoded products with 71% amino acid identity; the umuC sequences were 71% homologous and encoded products with 83% amino acid identity. Furthermore, the potential UmuD cleavage site and associated catalytic sites could be identified. Thus the very different mutagenic responses of S. typhimurium LT2 and E. coli cannot be accounted for by gross differences in operon structure or gene products. Rather, the ability of the cloned S. typhimurium umuD gene to give stronger complementation of E. coli umuD77 mutants in the absence of a functional umuC gene suggests that Salmonella UmuC protein normally constrains UmuD protein activity

Ripple design of LT codes for AWGN channel

DEFF Research Database (Denmark)

Sørensen, Jesper Hemming; Koike-Akino, Toshiaki; Orlik, Philip

2012-01-01

In this paper, we present an analytical framework for designing LT codes in additive white Gaussian noise (AWGN) channels. We show that some of analytical results from binary erasure channels (BEC) also hold in AWGN channels with slight modifications. This enables us to apply a ripple-based design...

Mutations in COL1A1 and COL1A2 and dental aberrations in children and adolescents with osteogenesis imperfecta - A retrospective cohort study.

Directory of Open Access Journals (Sweden)

Kristofer Andersson

Full Text Available Osteogenesis imperfecta (OI is a heterogeneous group of disorders of connective tissue, caused mainly by mutations in the collagen I genes (COL1A1 and COL1A2. Dentinogenesis imperfecta (DGI and other dental aberrations are common features of OI. We investigated the association between collagen I mutations and DGI, taurodontism, and retention of permanent second molars in a retrospective cohort of 152 unrelated children and adolescents with OI. The clinical examination included radiographic evaluations. Teeth from 81 individuals were available for histopathological evaluation. COL1A1/2 mutations were found in 104 individuals by nucleotide sequencing. DGI was diagnosed clinically and radiographically in 29% of the individuals (44/152 and through isolated histological findings in another 19% (29/152. In the individuals with a COL1A1 mutation, 70% (7/10 of those with a glycine substitution located C-terminal of p.Gly305 exhibited DGI in both dentitions while no individual (0/7 with a mutation N-terminal of this point exhibited DGI in either dentition (p = 0.01. In the individuals with a COL1A2 mutation, 80% (8/10 of those with a glycine substitution located C terminal of p.Gly211 exhibited DGI in both dentitions while no individual (0/5 with a mutation N-terminal of this point (p = 0.007 exhibited DGI in either dentition. DGI was restricted to the deciduous dentition in 20 individuals. Seventeen had missense mutations where glycine to serine was the most prevalent substitution (53%. Taurodontism occurred in 18% and retention of permanent second molars in 31% of the adolescents. Dental aberrations are strongly associated with qualitatively changed collagen I. The varying expressivity of DGI is related to the location of the collagen I mutation. Genotype information may be helpful in identifying individuals with OI who have an increased risk of dental aberrations.

Containment pressure analysis model using CONTEMPT-LT

International Nuclear Information System (INIS)

Gupta, R.N.

1975-09-01

An analytical model for evaluating the reactor containment pressure transient following a loss-of-coolant accident (LOCA) is presented. The model uses the CONTEMPT-LT computer program developed by Aerojet Nuclear Company. The sample problem studied is the containment response following the most severe postulated LOCA at the Yankee Rowe Nuclear Power Station. The results show good agreement with the response predicted by Westinghouse Electric Corporation. (auth)

A COL11A2 mutation in Labrador retrievers with mild disproportionate dwarfism.

Science.gov (United States)

Frischknecht, Mirjam; Niehof-Oellers, Helena; Jagannathan, Vidhya; Owczarek-Lipska, Marta; Drögemüller, Cord; Dietschi, Elisabeth; Dolf, Gaudenz; Tellhelm, Bernd; Lang, Johann; Tiira, Katriina; Lohi, Hannes; Leeb, Tosso

2013-01-01

We describe a mild form of disproportionate dwarfism in Labrador Retrievers, which is not associated with any obvious health problems such as secondary arthrosis. We designate this phenotype as skeletal dysplasia 2 (SD2). It is inherited as a monogenic autosomal recessive trait with incomplete penetrance primarily in working lines of the Labrador Retriever breed. Using 23 cases and 37 controls we mapped the causative mutation by genome-wide association and homozygosity mapping to a 4.44 Mb interval on chromosome 12. We re-sequenced the genome of one affected dog at 30x coverage and detected 92 non-synonymous variants in the critical interval. Only two of these variants, located in the lymphotoxin A (LTA) and collagen alpha-2(XI) chain gene (COL11A2), respectively, were perfectly associated with the trait. Previously described COL11A2 variants in humans or mice lead to skeletal dysplasias and/or deafness. The dog variant associated with disproportionate dwarfism, COL11A2:c.143G>C or p.R48P, probably has only a minor effect on collagen XI function, which might explain the comparatively mild phenotype seen in our study. The identification of this candidate causative mutation thus widens the known phenotypic spectrum of COL11A2 mutations. We speculate that non-pathogenic COL11A2 variants might even contribute to the heritable variation in height.

General Urban Warfare Amphibious Logistics Applications. Volume 1. Technical Report.

Science.gov (United States)

1983-06-23

condiments , tea, powdered milk, etc., but these will probably he available in the local economy. If not, and if a determination i. made to provide any of...March, 1978, pp. 78-83. Marsh, LtCol J.O., McCormick , lstLt M., et al. "Combat Support: Shore Party and Logistics; Engineer; Communications Air

Annual Report of the Chief, National Guard Bureau for the Fiscal Year 1970

Science.gov (United States)

1970-06-30

Arizona Maj. Gen. Jackson Bogle Arkansas Maj. Gen. Charles H. Wilson California Maj. (Jen. Clenn C. Ames Color,idd Maj. Gen. )oe C. Moffitt...Lynwood F. Hoxsie South Carolina Col. James C. Dozier South Dakota Maj. Edward P. Rowen Tennessee Col. Percy C Miller Texas Col. Vernon M. Schofield...Johnny J. PSG. ARNG. Illinois Huebner, Herman H. Sp4. ARNG. California Jackson , Paul N. Cap».. AflNG, North Carolina Kauhaihao, John K. 1st Lt

COL Application Content Guide for HTGRs: Revision to RG 1.206, Part 1 - Status Report

Energy Technology Data Exchange (ETDEWEB)

Wayne Moe

2012-08-01

A combined license (COL) application is required by the Nuclear Regulatory Commission (NRC) for all proposed nuclear plants. The information requirements for a COL application are set forth in 10 CFR 52.79, “Contents of Applications; Technical Information in Final Safety Analysis Report.” An applicant for a modular high temperature gas-cooled reactor (HTGR) must develop and submit for NRC review and approval a COL application which conforms to these requirements. The technical information necessary to allow NRC staff to evaluate a COL application and resolve all safety issues related to a proposed nuclear plant is detailed and comprehensive. To this, Regulatory Guide (RG) 1.206, “Combined License Applications for Nuclear Power Plants” (LWR Edition), was developed to assist light water reactor (LWR) applicants in incorporating and effectively formatting required information for COL application review (Ref. 1). However, the guidance prescribed in RG 1.206 presumes a LWR design proposal consistent with the systems and functions associated with large LWR power plants currently operating under NRC license.

CHROMagar COL-APSE: a selective bacterial culture medium for the isolation and differentiation of colistin-resistant Gram-negative pathogens

DEFF Research Database (Denmark)

Abdul Momin, Muhd Haziq F; Bean, David C; Hendriksen, Rene S.

2017-01-01

medium (SuperPolymyxin). Methodology. The medium was challenged with 84 isolates, including polymyxin B (POL B)-susceptible and -resistant type strains and colistin (COL)-resistant organisms recovered from human and animal samples. Susceptibility to COL and POL B was determined by agar dilution and broth...... microtitre dilution. The lower limit for the detection of COL-resistant organisms was also calculated for both CHROMagar COL-APSE and SuperPolymyxin media. The ability to isolate and correctly differentiate COL-resistant organisms within mixed cultures was also assessed and compared using both media. Results...

The Coast Artillery Journal. Volume 78, Number 5, September-October 1935

Science.gov (United States)

1935-10-01

entire area seemed deserted bue other watchers, as did I, knew better. I / walked back to the tank where it nestled • in a hollow and told Ridout to move...Association appointed a board of officersconsisting of: Lt. Col. F. P. Hardaway, c.A.c., Lt. Co!. E. B. Gray, C.A.C., and Major Milo Brinkley, CA-Res

Variability of Two Young L/T Transition Brown Dwarfs

Science.gov (United States)

Allers, Katelyn; Biller, Beth; Gallimore, Jack; Crossfield, Ian

2015-10-01

We propose for photometric monitoring observations of WISEP J004701.06+680352 (hereinafter W0047) and 2MASSWJ2244316+204343 (hereinafter 2M2244) using Spitzer/IRAC. Both objects are kinematically confirmed L7 members of the 150 Myr old AB Doradus moving group and show remarkable spectral similarity in both the near-IR and optical. The WoW survey found that L/T transition brown dwarfs having detected mid-IR variability are redder than the typical J - K color for their spectral type. A Cycle 11 exploration program (P.I. Metchev) is investigating the geometrical dependence of color and variability by expanding the original WoW sample. If inclination and J - K color are correlated (as predicted by Metchev et al.), then the spectral and photometric diversity seen across the L/T transition could be explained by geometry rather than diversity in atmospheric chemistry and dynamics. This would have wide ranging implications for the way we model cloud dissipation for brown dwarfs and extrasolar planets. Our proposed observations will provide an important test of the Metchev et al. prediction complementary to their Cycle 11 program. W0047 and 2M2244 are the same age, and have remarkably similar colors (J - K = 2.55 and 2.46 mags, respectively) and underlying spectra. Thus, if Metchev's prediction about the correlation of inclination and spectral morphology holds true, we would expect that W0047 and 2M2244 should have similar inclinations. However, the measured v sin(i) values for W0047 and 2M2244 are quite different. This difference in v sin(i) could be due to spin-axis inclination (with W0047 having a smaller i) or orbital period (with W0047 having a longer period), both of which we will determine from our proposed observations. This test is a unique opportunity, as there are no other free-floating L/T transition dwarfs known to be both coeval and spectrally similar. Our proposed observations will also extend the spectral type range for young objects surveyed for variability

A COL11A2 mutation in Labrador retrievers with mild disproportionate dwarfism.

Directory of Open Access Journals (Sweden)

Mirjam Frischknecht

Full Text Available We describe a mild form of disproportionate dwarfism in Labrador Retrievers, which is not associated with any obvious health problems such as secondary arthrosis. We designate this phenotype as skeletal dysplasia 2 (SD2. It is inherited as a monogenic autosomal recessive trait with incomplete penetrance primarily in working lines of the Labrador Retriever breed. Using 23 cases and 37 controls we mapped the causative mutation by genome-wide association and homozygosity mapping to a 4.44 Mb interval on chromosome 12. We re-sequenced the genome of one affected dog at 30x coverage and detected 92 non-synonymous variants in the critical interval. Only two of these variants, located in the lymphotoxin A (LTA and collagen alpha-2(XI chain gene (COL11A2, respectively, were perfectly associated with the trait. Previously described COL11A2 variants in humans or mice lead to skeletal dysplasias and/or deafness. The dog variant associated with disproportionate dwarfism, COL11A2:c.143G>C or p.R48P, probably has only a minor effect on collagen XI function, which might explain the comparatively mild phenotype seen in our study. The identification of this candidate causative mutation thus widens the known phenotypic spectrum of COL11A2 mutations. We speculate that non-pathogenic COL11A2 variants might even contribute to the heritable variation in height.

USAF Hearing Conservation Program, DOEHRS-HC Data Repository Annual Report: CY15

Science.gov (United States)

2017-05-31

does not license the holder or any other person or corporation or convey any rights or permission to manufacture, use, or sell any patented invention...be aware that notwithstanding any other provision of law , no person shall be subject to any penalty for failing to comply with a collection of...purpose of this report is to provide a corporate view of the status of the USAF HCP with data reported from the Defense Occupational and Environmental

Corrections and additions to CONTEMPT-LT computer codes for containment analysis

International Nuclear Information System (INIS)

Eerikaeinen, Lauri.

1980-01-01

The report presents a new version of CONTEMPT-LT/26 tainment code. The corrections and additions are applicable also to other CONTEMPT-LT versions. Thermodynamical background of corrections are shortly described, and in addition, some essential points which should be taken into account in the analysis of a pressure suppression containment have been pointed out. The results obtained by the corrected version have been compared to those calculated by the original program, and to the measured data in the Marviken containment experiment No 10. Finally, it has been indicated that for reliable pressure suppression analysis a wide ranging condensation model for air-steam mixture is necessary. (author)

Air & Space Power Journal. Volume 21, Number 4, Winter 2007

Science.gov (United States)

2007-01-01

Col Robyn Read, USAF, Retired 42 Chinese Servicemen’s...flying with hand move­ ments (“shooting their watch”), and sipping tea from porcelain glasses— a scene typical of flying squadrons around the world...opportunities in Arabic, Chinese , english, french, portuguese, and spanish. to submit an article in any of these languages, please re­ fer to the submission

Diferença no tamanho de cascas de ovos de colônias de Panstrongylus megistus

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Rosa João A da

2003-01-01

Full Text Available O objetivo do estudo foi avaliar as mensurações de cascas de ovos de duas colônias laboratoriais e uma silvestre de P. megistus. De cada uma das colônias, 50 cascas de ovos foram mensuradas por meio de microscópio estereoscópico e sistema de análise de imagem QWin. A análise estatística foi efetuada utilizando o teste t-Student, não pareado no programa INSTAT. A largura, o comprimento e o diâmetro da abertura opercular das cascas de ovos de uma colônia silvestre foram maiores que de duas colônias laboratoriais de P. megistus.

Type II heat-labile enterotoxins from 50 diverse Escherichia coli isolates belong almost exclusively to the LT-IIc family and may be prophage encoded.

Directory of Open Access Journals (Sweden)

Michael G Jobling

Full Text Available Some enterotoxigenic Escherichia coli (ETEC produce a type II heat-labile enterotoxin (LT-II that activates adenylate cyclase in susceptible cells but is not neutralized by antisera against cholera toxin or type I heat-labile enterotoxin (LT-I. LT-I variants encoded by plasmids in ETEC from humans and pigs have amino acid sequences that are ≥ 95% identical. In contrast, LT-II toxins are chromosomally encoded and are much more diverse. Early studies characterized LT-IIa and LT-IIb variants, but a novel LT-IIc was reported recently. Here we characterized the LT-II encoding loci from 48 additional ETEC isolates. Two encoded LT-IIa, none encoded LT-IIb, and 46 encoded highly related variants of LT-IIc. Phylogenetic analysis indicated that the predicted LT-IIc toxins encoded by these loci could be assigned to 6 subgroups. The loci corresponding to individual toxins within each subgroup had DNA sequences that were more than 99% identical. The LT-IIc subgroups appear to have arisen by multiple recombinational events between progenitor loci encoding LT-IIc1- and LT-IIc3-like variants. All loci from representative isolates encoding the LT-IIa, LT-IIb, and each subgroup of LT-IIc enterotoxins are preceded by highly-related genes that are between 80 and 93% identical to predicted phage lysozyme genes. DNA sequences immediately following the B genes differ considerably between toxin subgroups, but all are most closely related to genomic sequences found in predicted prophages. Together these data suggest that the LT-II loci are inserted into lambdoid type prophages that may or may not be infectious. These findings raise the possibility that production of LT-II enterotoxins by ETEC may be determined by phage conversion and may be activated by induction of prophage, in a manner similar to control of production of Shiga-like toxins by converting phages in isolates of enterohemmorhagic E. coli.

[Survival properties of ETEC surface-displayed K88ac-LT(B) on Lactobacillus casei].

Science.gov (United States)

Wei, Chunhua; Liu, Jiankui; Hou, Xilin; Wang, Guihua; Yu, Liyun

2009-01-01

K88ac-LT(B) gene derived from pQE30-K88ac-LT(B) was cloned into the expression vector pLA and then the recombinant vector was transformed into the competent cells Lactobacillus casei 525. The recombinant bacteria were grown at 37 degrees C, in MRS broth. Western blotting analysis with rabbit-anti-K88ac-LT(B) polyclonal serum indicated that the recombinant protein reacted with the specific antibodies. The results showed that the molecular weight of the recombinant protein was about 71.2 kD. The K88ac-LT(B) fusion protein on the cell surface was confirmed by immunofluorescence mciroscopy and flow cytometric analysis. In addition, the survival of recombinant Lactobacillus casei 525 was studied in imitative gastrointestinal environments such as artificial gastro fluid (pH 1.5-5.5), artificial intestinal fluid, bile(0.3-3.0 g/L). The results indicated that the recombinant strain survived well in artificial gastric fluids at pH 2.5-4.5 in 5 h. The recombinant Lactobacillus casei 525 could slowly grow in the artificial intestinal fluid for different time, and could survive in 0.3% bile.

An apple B-box protein, MdCOL11, is involved in UV-B- and temperature-induced anthocyanin biosynthesis.

Science.gov (United States)

Bai, Songling; Saito, Takanori; Honda, Chikako; Hatsuyama, Yoshimichi; Ito, Akiko; Moriguchi, Takaya

2014-11-01

Our studies showed that an apple B-box protein, MdCOL11, the homolog of AtBBX22, is involved in UV-B- and temperature-induced anthocyanin biosynthesis in apple peel. Anthocyanin is responsible for the red pigmentation in apple peel and a R2R3 MYB gene, MdMYBA/1/10, a homolog of MdMYBA, controls its accumulation. Arabidopsis PAP1 is under the control of a series of upstream factors involved in light signal transduction and photomorphogenesis, such as ELONGATED HYPOCOTYL 5 (HY5) and B-box family (BBX) proteins. In this study, we identified and characterized the homolog of Arabidopsis BBX22 in apple, designated as MdCOL11. Overexpression of MdCOL11 in Arabidopsis enhanced the accumulation of anthocyanin. In apples, MdCOL11 was differentially expressed in all tissues, with the highest expression in petals and the lowest expression in the xylem. Transcripts of MdCOL11 noticeably accumulated at the ripening stage, concomitant with increases in the expressions of anthocyanin biosynthesis-related genes. In an in vitro treatment experiment, MdCOL11 was upregulated in an ultra-violet (UV)-B- and temperature-dependent manner, together with the inductions of anthocyanin biosynthesis-related genes and anthocyanin accumulation in apple peel. Furthermore, a dual-luciferase assay indicated that (1) MdCOL11 regulated the expression of MdMYBA and (2) MdCOL11 was a target of MdHY5. Taken together, our results suggest that MdCOL11 is involved in MdHY5-mediated signal transduction and regulates anthocyanin accumulation in apple peel, which sheds new light on anthocyanin accumulation in apples.

From the Underground to the High Ground: The Insurgent Use of Airpower

Science.gov (United States)

2011-02-16

government. Contents Disclaimer……………………………………………………………………………….....i Contents…………………………………………………………………………………...ii Biography ...19 Biography Lt. Col. John G. Bunnell is a student at Air War College. Prior to Air University, Lt. Col. Bunnell served as an F...The Jerusalem Post, 19 April 2005. 45 Lisa Myers, “Hezbollah drone threatens Israel,” NBC Nightly News, 4 December 2005. 46 Roee Nahmias

Japanese Studies on Manchuria. Volume 11. Part 3. Book B. Small Wars and Border Problems, The Nomonhan Incident

Science.gov (United States)

1956-09-05

ranks and posts as of the time of the Nomonhan Incident): UaJ. Gen. Yuzaburo Hata, CG, 3d Heavy Field Artillery Brigade MaJ. Gen. Kotoku Sato , CG...111.. 1 II ^72 Col Mikio Sakoi I {X 23 Lt Col Yoozo Azumo _L [j Co 11. . 1 23 Tokahide Ise I I II 23 Isamu Saito fHBCSüJ

Case of Small Vessel Disease Associated with COL4A1 Mutations following Trauma

Directory of Open Access Journals (Sweden)

Joao McONeil Plancher

2015-06-01

Full Text Available With this case report, we would like to heighten the awareness of clinicians about COL4A1 as a single-gene disorder causing cerebral small vessel disease and describe a previously unreported pathogenic missense substitution in COL4A1 (p.Gly990Val and a new clinical presentation. We identified a heterozygous putatively pathogenic mutation of COL4A1 in a 50-year-old female with a history of congenital cataracts and glaucoma who presented with multiple diffusion-positive infarcts and areas of contrast enhancement following mild head trauma. We believe that this presentation of multiple areas of acute brain and vascular injury in the setting of mild head trauma is a new manifestation of this genetic disorder. Imaging findings of multiple acute infarcts and regions of contrast enhancement with associated asymptomatic old deep microhemorrhages and leukomalacia in adults after head trauma should raise a high suspicion for a COL4A1 genetic disorder. Radiographic patterns of significant leukoaraiosis and deep microhemorrhages can also be seen in patients with long-standing vasculopathy associated with hypertension, which our patient lacked. Our findings demonstrate the utility of genetic screening for COL4A1 mutations in young patients who have small vessel vasculopathy on brain imaging but who do not have significant cardiovascular risk factors.

Use of Information Technology Tools in Source Selection Decision Making: A Study on USAF's KC-X Tanker Replacement Program

National Research Council Canada - National Science Library

Kaymaz, Sidar; Diri, Alaattin

2008-01-01

... and subjectivity is usually inevitable in this kind of a decision making process. The purpose of this project is to demonstrate how the USAF's current source selection method (color rating method...

CHROMagar COL-APSE: a selective bacterial culture medium for the isolation and differentiation of colistin-resistant Gram-negative pathogens

DEFF Research Database (Denmark)

Abdul Momin, Muhd Haziq F; Bean, David C; Hendriksen, Rene S.

2017-01-01

Purpose. A selective chromogenic culture medium for the laboratory isolation and differentiation of colistin resistant Acinetobacter, Pseudomonas, Stenotrophomonas and Enterobacteriaceae spp. (CHROMagar COL-APSE) was developed, evaluated and compared to an existing selective bacterial culture......-resistant non-fermentative bacteria (Acinetobacter, Pseudomonas and Stenotrophomonas). CHROMagar COL-APSE was also more sensitive in supporting the growth of Enterobacteriaceae with COL resistance associated with the carriage of mcr-1. Conclusion. CHROMagar COL-APSE is a sensitive and specific medium...

Severe Hemolytic Jaundice in a Neonate with a Novel COL4A1 Mutation.

Science.gov (United States)

Tomotaki, Seiichi; Mizumoto, Hiroshi; Hamabata, Takayuki; Kumakura, Akira; Shiota, Mitsutaka; Arai, Hiroshi; Haginoya, Kazuhiro; Hata, Daisuke

2016-12-01

We report our experience with a preterm infant with severe hemolytic jaundice who required exchange transfusion just after birth. The patient was negative for alloimmune hemolysis as a result of maternal-fetal blood type incompatibility, and tests for inherited defects in erythrocyte metabolism, membrane function, and hemoglobin synthesis were normal. We also performed a bone marrow examination, but could not identify the cause of hemolysis. The patient had several other complications, including porencephaly, epilepsy, elevated serum levels of creatine kinase, and persistent microscopic hematuria. Later, we detected a genetic mutation in COL4A1, which was recently found to be associated with hemolytic anemia. We therefore believe that all of the patient's clinical features, including hemolytic anemia, were due to the mutation in COL4A1. Genetic testing for COL4A1 mutations is recommended in neonates who exhibit hemolytic disease of unknown etiology, especially when other complications compatible with COL4A1-related disorders are present. Copyright © 2014. Published by Elsevier B.V.

COL-3, a chemically modified tetracycline, inhibits lipopolysaccharide-induced microglia activation and cytokine expression in the brain.

Directory of Open Access Journals (Sweden)

Rawan Abdulhameed Edan

Full Text Available Microglia activation results in release of proinflammatory molecules including cytokines, which contribute to neuronal damage in the central nervous system (CNS if not controlled. Tetracycline antibiotics such as minocycline inhibit microglial activation and cytokine expression during CNS inflammation. In the present study we found that administration of chemically modified tetracycline-3 (COL-3, inhibits lipopolysaccharide (LPS-induced microglial and p38 MAPK activation, as well as the increase in TNF-α, but not IL-1β expression, in the brains of BALB/c mice. COL-3 has been described to have no antibacterial activity. We observed that COL-3 had no activity against a Gram-negative bacteria, Escherichia coli; however surprisingly, COL-3 had antibacterial activity against a Gram-positive bacteria Staphylococcus aureus, with a minimum inhibitory concentration of 1 mg/ml. Our data show that COL-3 has some antibacterial activity against S. aureus, inhibits LPS-induced neuroinflammation, and displays potential as a therapeutic agent for treatment of conditions involving CNS inflammation.

Evolution of Regions Containing Antibiotic Resistance Genes in FII-2-FIB-1 ColV-Colla Virulence Plasmids.

Science.gov (United States)

Moran, Robert A; Hall, Ruth M

2018-05-01

Three ColV virulence plasmids carrying antibiotic resistance genes were assembled from draft genome sequences of commensal ST95, ST131, and ST2705 Escherichia coli isolates from healthy Australians. Plasmids pCERC4, pCERC5, and pCERC9 include almost identical backbones containing FII-2 and FIB-1 replicons and the conserved ColV virulence region with an additional ColIa determinant. Only pCERC5 includes a complete, uninterrupted F-like transfer region and was able to conjugate. pCERC5 and pCERC9 contain Tn1721, carrying the tet(A) tetracycline resistance determinant in the same location, with Tn2 (bla TEM ; ampicillin resistance) interrupting the Tn1721 in pCERC5. pCERC4 has a Tn1721/Tn21 hybrid transposon carrying dfrA5 (trimethoprim resistance) and sul1 (sulfamethoxazole resistance) in a class 1 integron. Four FII-2:FIB-1 ColV-ColIa plasmids in the GenBank nucleotide database have a related transposon in the same position, but an IS26 has reshaped the resistance gene region, deleting 2,069 bp of the integron 3'-CS, including sul1, and serving as a target for IS26 translocatable units containing bla TEM , sul2 and strAB (streptomycin resistance), or aphA1 (kanamycin/neomycin resistance). Another ColV-ColIa plasmid containing a related resistance gene region has lost the FII replicon and acquired a unique transfer region via recombination within the resistance region and at oriT. Eighteen further complete ColV plasmid sequences in GenBank contained FIB-1, but the FII replicons were of three types, FII-24, FII-18, and a variant of FII-36.

Potential probiotic of Lactobacillus johnsonii LT171 for chicken ...

African Journals Online (AJOL)

The objective of this research was to investigate the potential probiotic of Lactobacillus johnsonii LT171. It had aggregation (60 min) and antibacterial effects against Salmonella Enteritidis, Salmonella Typhimurium and Escherichia coli O78:K80. It showed amylase and protease activity and high clear zone in culture medium ...

Bone mineral properties in growing Col1a2(+/G610C) mice, an animal model of osteogenesis imperfecta.

Science.gov (United States)

Masci, Marco; Wang, Min; Imbert, Laurianne; Barnes, Aileen M; Spevak, Lyudmila; Lukashova, Lyudmila; Huang, Yihe; Ma, Yan; Marini, Joan C; Jacobsen, Christina M; Warman, Matthew L; Boskey, Adele L

2016-06-01

The Col1a2(+/G610C) knock-in mouse, models osteogenesis imperfecta in a large old order Amish family (OOA) with type IV OI, caused by a G-to-T transversion at nucleotide 2098, which alters the gly-610 codon in the triple-helical domain of the α2(I) chain of type I collagen. Mineral and matrix properties of the long bones and vertebrae of male Col1a2(+/G610C) and their wild-type controls (Col1a2(+/+)), were characterized to gain insight into the role of α2-chain collagen mutations in mineralization. Additionally, we examined the rescuability of the composition by sclerostin inhibition initiated by crossing Col1a2(+/G610C) with an LRP(+/A214V) high bone mass allele. At age 10-days, vertebrae and tibia showed few alterations by micro-CT or Fourier transform infrared imaging (FTIRI). At 2-months-of-age, Col1a2(+/G610C) tibias had 13% fewer secondary trabeculae than Col1a2(+/+), these were thinner (11%) and more widely spaced (20%) than those of Col1a2(+/+) mice. Vertebrae of Col1a2(+/G610C) mice at 2-months also had lower bone volume fraction (38%), trabecular number (13%), thickness (13%) and connectivity density (32%) compared to Col1(a2+/+). The cortical bone of Col1a2(+/G610C) tibias at 2-months had 3% higher tissue mineral density compared to Col1a2(+/+); Col1a2(+/G610C) vertebrae had lower cortical thickness (29%), bone area (37%) and polar moment of inertia (38%) relative to Col1a2(+/+). FTIRI analysis, which provides information on bone chemical composition at ~7μm-spatial resolution, showed tibias at 10-days did not differ between genotypes. Comparing identical bone types in Col1a2(+/G610C) to Col1a2(+/+) at 2-months-of-age, tibias showed higher mineral-to-matrix ratio in trabeculae (17%) and cortices (31%). and in vertebral cortices (28%). Collagen maturity was 42% higher at 10-days-of-age in Col1a2(+/G610C) vertebral trabeculae and in 2-month tibial cortices (12%), vertebral trabeculae (42%) and vertebral cortices (12%). Higher acid-phosphate substitution

Investigation of Public Charging Infrastructure : Case study Gränby sportfält

OpenAIRE

Dahl, Emma; Hedström, Andreas; Lindgren, Anna

2017-01-01

The municipal company Sportfastigheter AB is currently renovating and developing Gränby sportfält, a sports field in Uppsala. Adjacent to the sports field, a parking lot for 700 vehicles is located, where Sportfastigheter AB is preparing to install charging points for electric vehicles (EVs) at some of the places. This bachelor thesis aims to investigate how a public charging solution should be modeled, with the parking lot at Gränby sportfält as a case study. The investigation involves estim...

Designing an efficient LT-code with unequal error protection for image transmission

Science.gov (United States)

S. Marques, F.; Schwartz, C.; Pinho, M. S.; Finamore, W. A.

2015-10-01

The use of images from earth observation satellites is spread over different applications, such as a car navigation systems and a disaster monitoring. In general, those images are captured by on board imaging devices and must be transmitted to the Earth using a communication system. Even though a high resolution image can produce a better Quality of Service, it leads to transmitters with high bit rate which require a large bandwidth and expend a large amount of energy. Therefore, it is very important to design efficient communication systems. From communication theory, it is well known that a source encoder is crucial in an efficient system. In a remote sensing satellite image transmission, this efficiency is achieved by using an image compressor, to reduce the amount of data which must be transmitted. The Consultative Committee for Space Data Systems (CCSDS), a multinational forum for the development of communications and data system standards for space flight, establishes a recommended standard for a data compression algorithm for images from space systems. Unfortunately, in the satellite communication channel, the transmitted signal is corrupted by the presence of noise, interference signals, etc. Therefore, the receiver of a digital communication system may fail to recover the transmitted bit. Actually, a channel code can be used to reduce the effect of this failure. In 2002, the Luby Transform code (LT-code) was introduced and it was shown that it was very efficient when the binary erasure channel model was used. Since the effect of the bit recovery failure depends on the position of the bit in the compressed image stream, in the last decade many e orts have been made to develop LT-code with unequal error protection. In 2012, Arslan et al. showed improvements when LT-codes with unequal error protection were used in images compressed by SPIHT algorithm. The techniques presented by Arslan et al. can be adapted to work with the algorithm for image compression

RNase H and replication of ColE1 DNA in Escherichia coli.

OpenAIRE

Naito, S; Uchida, H

1986-01-01

Amber mutations within the rnh (RNase H) gene of Escherichia coli K-12 were isolated by selecting for bacteria capable of replicating in a sup+ background replication-defective cer-6 mutant of the ColE1 replicon. The cer-6 mutation is an alteration of one base pair located 160 nucleotides upstream of the unique replication origin of this plasmid. Subsequently, we determined the DNA alterations present within these mutants. ColE1 DNA replicated in rnh(Am) recA cells, indicating that (i) RNase ...

Kültür ve Sanat Ekonomisi Kapsamında Talep Analizleri Yardımı ile Kültür Politikasına Teorik Yaklaşım: Türkiye Örneği

Directory of Open Access Journals (Sweden)

Duygu BÜYÜKYAZICI

2016-08-01

Full Text Available Kültür-sanat ekonomisinin bağımsız bir disiplin olarak ortaya çıkmasını sağlayantemel tez, devletin sanatı desteklemesi gerektiği önermesidir. Gösteri sanatı formlarınıniktisadi araçlarla incelenmesi sonucunda ortaya konan maliyet hastalığı argümanı, devletsanatları desteklemezse sanatların yok olacağı öngörüsünü içerir. Söz konusu analizlerdenönce devlet-sanat ilişkisi farklı disiplinlerin gözlem alanında iken, bu değişkenlerin yanınaekonomi teriminin eklenmesi tartışmaları bilimsel zemine çekmiş ve engin incelemeleriberaberinde getirmiştir. Devletin finansal ve kurumsal anlamda sanatı desteklemesi politikayapıcıların nezdinde erdemli mallar varsayımı ile gerçekleşirken, literatürdekitemellendirmeler büyük ölçüde piyasa başarısızlıkları ekseninde olmuş ve maliyet hastalığıargümanı çürütülmüştür. Devletin sanatı destekleme formu ve miktarına yönelik tercihlerkültür politikasının önemli payını oluştururken, söz konusu politikaların edinilmiş başarısıancak talep analizleri ile tartılabilir. Türkiye’nin kültür politikasına hem teorik biryaklaşımda bulunmayı hem de ulaşılmış başarısını araştırmayı amaçlayan buçalışmada, kültür-sanat ekonomisindeki mikroekonomik talep analizlerinden yola çıkarak,talep profilinin kültür politikasındaki önemi vurgulanmakta ve devletin kültür-sanata olandesteği kadar biçiminin öneminin de altı çizilmektedir. Talep analizlerinin gelişmesidoğrultusunda, talebin profiline yönelik farklı kültür politikaları gerekecek, kültür-sanatayönelik çoğunlukla yetersiz veri ve analiz ile tespit edilen destek, üretim vesübvansiyonların içerik ve miktarı farklılaşarak optimal düzeye yakınsanacaktır. Piyasabaşarısızlıklar da kültür politikasının temellendiği teorik faz olarak analize eklenmiş, pozitifdışsallıklar özelinde devletin sanatı desteklemesinin sebepleri

Fetal origin of brain damage in 2 infants with a COL4A1 mutation: fetal and neonatal MRI

NARCIS (Netherlands)

Vermeulen, R. J.; Peeters-Scholte, C.; van Vugt, J. J. M.; van Vught, J. J. M. G.; Barkhof, F.; Rizzu, P.; van der Schoor, S. R. D.; van der Knaap, M. S.

2011-01-01

Mutations in the gene COL4A1, encoding collagen IV A1, are associated with familial porencephaly. Previously, COL4A1 mutation-associated antenatal hemorrhages have been suggested by early post-natal imaging. We describe 2 children with fetal intracerebral hemorrhages and a COL4A1 mutation. There was

Variação do volume de gotas de colírios lubrificantes disponíveis no mercado brasileiro

Directory of Open Access Journals (Sweden)

Vitorugo Silvestre Nascimento

Full Text Available RESUMO Objetivo: Avaliar a variação intra e interexaminadores do volume de gotas dispensados de frascos de colírios lubrificantes disponíveis no mercado. Métodos: Foram estudados cinco frascos de colírios lubrificantes e dezenove voluntários participaram deste estudo. A massa média de gotas de 20µl dos colírios foi obtida utilizando micropipeta e balança de precisão e como padrão para comparação com a massa das gotas obtidas pelos voluntários. Cinco gotas de cada frasco foram pesadas individualmente com o tubo de colírio perpendicular à balança, usando o primeiro e segundo dedos da mão direita, de forma que a pressão fosse aplicada somente no meio do frasco. Os experimentos foram realizados em uma sala climatizada a temperatura ambiente (21±1°C. Resultados: Todos os frascos de colírios apresentaram variação estatisticamente significante das massas das gotas obtidas pelos examinadores quando comparadas com a massa média padrão de 0,0182±0,0014g, com exceção da comparação entre os dados do colírio A com o colírio D, que não apresentou variação estatisticamente significante. Conclusão: O presente estudo demonstra a ausência de uniformidade das gotas dispensadas pelos frascos de colírios disponíveis no mercado e a sua inadequação à real necessidade, uma vez que as gotas dispensadas são maiores do que o indicado. Esse fato torna-se um problema quando se trata de período de tratamento prolongado, especialmente com colírios dispendiosos como os indicados para a terapêutica do glaucoma. Nesse sentido, a padronização das gotas de colírios se faz necessária.

Using the Freiburg Acuity and Contrast Test to measure visual performance in USAF personnel after PRK.

Science.gov (United States)

Dennis, Richard J; Beer, Jeremy M A; Baldwin, J Bruce; Ivan, Douglas J; Lorusso, Frank J; Thompson, William T

2004-07-01

Photorefractive keratectomy (PRK) may be an alternative to spectacle and contact lens wear for United States Air Force (USAF) aircrew and may offer some distinct advantages in operational situations. However, any residual corneal haze or scar formation from PRK could exacerbate the disabling effects of a bright glare source on a complex visual task. The USAF recently completed a longitudinal clinical evaluation of the long-term effects of PRK on visual performance, including the experiment described herein. After baseline data were collected, 20 nonflying active duty USAF personnel underwent PRK. Visual performance was then measured at 6, 12, and 24 months after PRK. Visual acuity (VA) and contrast sensitivity (CS) data were collected by using the Freiburg Acuity and Contrast Test (FrACT), with the subject viewing half of the runs through a polycarbonate windscreen. Experimental runs were completed under 3 glare conditions: no glare source and with either a broadband or a green laser (532-nm) glare annulus (luminance approximately 6090 cd/m) surrounding the Landolt C stimulus. Systematic effects of PRK on VA relative to baseline were not identified. However, VA was almost 2 full Snellen lines worse with the laser glare source in place versus the broadband glare source. A significant drop-off was observed in CS performance after PRK under conditions of no glare and broadband glare; this was the case both with and without the windscreen. As with VA, laser glare disrupted CS performance significantly and more than broadband glare did. PRK does not appear to have affected VA, but the changes in CS might represent a true decline in visual performance. The greater disruptive effects from laser versus broadband glare may be a result of increased masking from coherent spatial noise (speckle) surrounding the laser stimulus.

Project AIR FORCE Modeling Capabilities for Support of Combat Operations in Denied Environments

Science.gov (United States)

2015-01-01

within the Air Force and OSD.7 First, we thank Generals Herbert Carlisle, PACAF/CC, and Janet Wolfenbarger, AFMC/CC, for sponsoring this work. Gen...A5X, Brig Gen (s) John Cherrey, AF/A5XS, Col Jordan Thomas, AFA5XS, Lt Col Nathan Mead , AF/A5XS, Col Rich Gannon, AF/A9F, Ray Miller, AF/A9FC...linear programming (LP), which was developed in the 1940s by George Danzig to solve problems relevant to military logistics. Setting up MIP is

Avaliação do custo de colírios lubrificantes a base exclusivamente de carboximetilcelulose no mercado brasileiro

Directory of Open Access Journals (Sweden)

Paulo Estacia

2013-10-01

Full Text Available OBJETIVO: Avaliar o custo do uso dos colírios lubrificantes mais utilizados no mercado nacional que apresentam na formulação o mesmo princípio ativo - carboximetilcelulose, considerando o número total de gotas presente em cada frasco dos colírios. MÉTODOS: Foi realizado um estudo experimental utilizando três frascos de cada um dos colírios Lacrifilm® (colírio 1 e Fresh Tears® (colírio 2. Para análise do custo dos colírios contou-se o número de gotas de cada frasco correlacionando os preços dos mesmos. O preço considerado para cada medicação foi o mínimo ao consumidor com alíquota de 17% publicado para o mês de janeiro de 2012. A análise estatística foi efetuada em SPSS® 18. A comparação das variáveis quantitativas analisadas foi procedida através do teste não paramétrico Mann-Whitney e correlação linear de Spearman, sendo considerada uma diferença estatisticamente significante um valor de probabilidade inferior a 0,05. RESULTADOS: Verificou-se que há diferença estatisticamente significativa entre as marcas de colírios avaliados com relação ao número total de gotas. O colírio 1 apresentou o maior número de gotas. CONCLUSÃO: O colírio 1, que tem o maior número de gotas por frasco, também é o que tem o preço mais acessível. Portanto, verifica-se que é o produto mais econômico, ou seja, o paciente pagará menos por cada gota.

Volume da gota dos colírios lubrificantes: estudo farmacoeconômico

Directory of Open Access Journals (Sweden)

Alexandre Xavier da Costa

2015-12-01

Full Text Available RESUMO Objetivo: Determinar o volume médio das gotas produzidas pelos colírios lubrificantes em diferentes ângulos de inclinação e determinar o custo médio do tratamento. Métodos: Determinação do volume da gota de 3 frascos originais dos colírios lubrificantes Artelac®, Hylo Comod®, Lacrima® Plus, Systane® UL, Lacrifilm®, Hyabak®, Lacribell®, Ecofilm®, Mirugell®, Plenigell®, Fresh Tears®, Optive® e Endura® à inclinação de 90º e 45º. Determinou-se o número médio de gotas em cada frasco e foi feita avaliação farmacoeconômica dos colírios. Resultados: O volume das gotas variou de 32,2 a 64,0 µL a 45o e de 29,1 a 65,1 µL a 90o. A diferença entre as gotas em cada inclinação foi de 2 a 24% e o custo anual dos colírios de acordo com a inclinação variou de R$2,73 a R$130,73. A Duração Máxima de Tratamento (DMT foi de 29,3 a 51,4 dias na inclinação de 45o, e de 28,8 a 48,4 dias a 90º, sendo que a diferença na DMT foi de 0,5 até 8 dias a mais ou a menos, de acordo com a marca. Conclusão: Nenhum dos colírios estudados apresentou gotas ideais para o olho humano, levando a um desperdício do produto e maior custo para o fabricante e para o consumidor. Percebemos que existe uma variação significativa no volume da gota de acordo com a inclinação do frasco, e que uma variação maior do que 10% traria impactos financeiros para o paciente.

Polarized Structure Function $\\sigma_{LT'}$ for $p({\\vec e},e'K^+)\\Lambda$ in the Nucleon Resonance Region

Energy Technology Data Exchange (ETDEWEB)

Nasseripour, Rakhsha; Raue, Brian; Ambrozewicz, Pawel; Carman, Daniel; Amaryan, Moscov; Amaryan, Moskov; Anciant, Eric; Anghinolfi, Marco; Asavapibhop, Burin; Asryan, Gegham; Audit, Gerard; Auger, Thierry; Avagyan, Harutyun; Baghdasaryan, Hovhannes; Baillie, Nathan; Ball, J.P.; Ball, Jacques; Ball, J.P.; Ball, Jacques; Ball, J.P.; Ball, Jacques; Ball, J.P.; Ball, Jacques; Baltzell, Nathan; Barrow, Steve; Battaglieri, Marco; Beard, Kevin; Bedlinskiy, Ivan; Bektasoglu, Mehmet; Bellis, Matthew; Benmouna, Nawal; Berman, Barry; Biselli, Angela; Blaszczyk, Lukasz; Bonner, Billy; Bouchigny, Sylvain; Boyarinov, Sergey; Bradford, Robert; Branford, Derek; Briscoe, William; Brooks, William; Burkert, Volker; Butuceanu, Cornel; Calarco, John; Careccia, Sharon; Casey, Liam; Cetina, Catalina; Chen, Shifeng; Cheng, Lu; Cole, Philip; Collins, Patrick; Coltharp, Philip; Cords, Dieter; Corvisiero, Pietro; Crabb, Donald; Crede, Volker; Dale, Daniel; Dashyan, Natalya; De Masi, Rita; De Vita, Raffaella; De Sanctis, Enzo; Degtiarenko, Pavel; Dennis, Lawrence; Deur, Alexandre; Dhuga, Kalvir; Dickson, Richard; Djalali, Chaden; Dodge, Gail; Doughty, David; Dragovitsch, Peter; Dugger, Michael; Dytman, Steven; Dzyubak, Oleksandr; Egiyan, Hovanes; Egiyan, Kim; Elfassi, Lamiaa; Elouadrhiri, Latifa; Eugenio, Paul; Fatemi, Renee; Fedotov, Gleb; Feldman, Gerald; Feuerbach, Robert; Forest, Tony; Fradi, Ahmed; Funsten, Herbert; Garcon, Michel; Gavalian, Gagik; Gevorgyan, Nerses; Gilfoyle, Gerard; Giovanetti, Kevin; Girard, Pascal; Girod, Francois-Xavier; Goetz, John; Gothe, Ralf; Gothe, Ralf; Griffioen, Keith; Guidal, Michel; Guillo, Matthieu; Guler, Nevzat; Guo, Lei; Gyurjyan, Vardan; Hafidi, Kawtar; Hakobyan, Hayk; Hanretty, Charles; Hardie, John; Heddle, David; Hersman, F.; Hicks, Kenneth; Hleiqawi, Ishaq; Holtrop, Maurik; Hu, Jicun; Hyde, Charles; Ilieva, Yordanka; Ireland, David; Ishkhanov, Boris; Isupov, Evgeny; Ito, Mark; Jenkins, David; Jo, Hyon-Suk; Johnstone, John; Joo, Kyungseon; Juengst, Henry; Kalantarians, Narbe; Kellie, James; Khandaker, Mahbubul; Kim, Kui; Kim, Kyungmo; Kim, Wooyoung; Klein, Andreas; Klein, Franz; Kossov, Mikhail; Krahn, Zebulun; Kramer, Laird; Kubarovsky, Valery; Kuhn, Joachim; Kuhn, Sebastian; Kuleshov, Sergey; Kuznetsov, Viacheslav; Lachniet, Jeff; Laget, Jean; Langheinrich, Jorn; Lawrence, David; Livingston, Kenneth; Lu, Haiyun; Lukashin, Konstantin; MacCormick, Marion; Manak, Joseph; Markov, Nikolai; Mattione, Paul; McAleer, Simeon; McKinnon, Bryan; McNabb, John; Mecking, Bernhard; Mestayer, Mac; Meyer, Curtis; Mibe, Tsutomu; Mikhaylov, Konstantin; Minehart, Ralph; Mirazita, Marco; Miskimen, Rory; Mokeev, Viktor; Moreno, Brahim; Moriya, Kei; Morrow, Steven; Moteabbed, Maryam; Mueller, James; Munevar Espitia, Edwin; Mutchler, Gordon; Nadel-Turonski, Pawel; Niccolai, Silvia; Niculescu, Gabriel; Niculescu, Maria-Ioana; Niczyporuk, Bogdan; Niroula, Megh; Niyazov, Rustam; Nozar, Mina; Osipenko, Mikhail; Ostrovidov, Alexander; Park, Kijun; Pasyuk, Evgueni; Paterson, Craig; Pereira, Sergio; Peterson, Gerald; Philips, Sasha; Pierce, Joshua; Pivnyuk, Nikolay; Pocanic, Dinko; Pogorelko, Oleg; Pozdnyakov, Sergey; Preedom, Barry; Price, John; Procureur, Sebastien; Prok, Yelena; Protopopescu, Dan; Qin, Liming; Riccardi, Gregory; Ricco, Giovanni; Ripani, Marco; Ritchie, Barry; Rosner, Guenther; Rossi, Patrizia; Rubin, Philip; Sabatie, Franck; Salamanca, Julian; Salgado, Carlos; Santoro, Joseph; Sapunenko, Vladimir; Sayre, Donald; Schumacher, Reinhard; Serov, Vladimir; Shafi, Aziz; Sharabian, Youri; Sharov, Dmitri; Shvedunov, Nikolay; Simionatto, Sebastio; Skabelin, Alexander; Smith, Elton; Smith, Lee; Sober, Daniel; Sokhan, Daria; Stavinsky, Aleksey; Stepanyan, Samuel; Stepanyan, Stepan; Stokes, Burnham; Stoler, Paul; Strakovski, Igor; Strauch, Steffen; Taiuti, Mauro; Taylor, Shawn; Tedeschi, David; Thoma, Ulrike; Thompson, Richard; Tkabladze, Avtandil; Tkachenko, Svyatoslav; Ungaro, Maurizio; Vineyard, Michael; Vlassov, Alexander; Wang,

2008-06-01

The first measurements of the polarized structure function $\\sigma_{LT'}$ for the reaction $p(\\vec e,e'K^+)\\Lambda$ in the nucleon resonance region are reported. Measurements are included from threshold up to $W$=2.05 GeV for central values of $Q^2$ of 0.65 and 1.00 GeV$^2$, and nearly the entire kaon center-of-mass angular range. $\\sigma_{LT'}$ is the imaginary part of the longitudinal-transverse response and is expected to be sensitive to interferences between competing intermediate s-channel resonances, as well as resonant and non-resonant processes. The results for $\\sigma_{LT'}$ are comparable in magnitude to previously reported results from CLAS for $\\sigma_{LT}$, the real part of the same response. An intriguing sign change in $\\sigma_{LT'}$ is observed in the high $Q^2$ data at $W\\approx 1.9$ GeV. Comparisons to several existing model predictions are shown.

Defense AT&L (Volume 35, Number 4, July-August 2006)

National Research Council Canada - National Science Library

2006-01-01

...: Project Management in Afghanistan," by Lt. Col. William T. Cooley, et al. -- Successful rebuilding of the Afghan National Army and Afghan National Police is critical to long-term U.S. security...

Twenty-Seven-Year Follow-Up of Vietnam Air War USAF POWs and Matched Controls Not Captured: A Qualitative Study.

Science.gov (United States)

Sledge, William; Rozanova, Julia; Dorset, Julianne

2018-01-01

To provide a follow up of a 1976 study of the impact of captivity on U.S. Air Force (USAF) POWs and USAF Controls matched for time in Southeast Asia, military rank and aircraft crew position. Qualitative study of replies to open ended questions of positive and negative changes due to their captivity/combat experiences made by participants (POWs and Controls) who replied in both 1976 and in 2003. Both groups acknowledged positive and negative effects of the experiences in 1976. In 1976 and 2003 the POWs mainly reported negative effects on career and family domains but positive effects of individual development and growth. Controls reported mild negative effects on family in 1976, and benefits to their careers and sense of self in both 1976 and 2003. Captivity during the Vietnam War for USAF included two types of extreme duress which were the incarceration itself; and the repatriation experience which entailed re-assimilation despite loss of occupation and disrupted families. Despite these obstacles, POWs exhibited substantial resilience in achieving self-growth and how they regarded themselves psychologically in comparison to their matched control fellow aviators who while also suffering a lesser separation from family, tended to prosper in their careers and were proud of their accomplishments. Long term separation from work, family and friends and the inability to return to their families and careers with the effectiveness demanded by their ambition were a more devastating ongoing consequence of their captivity than the immediate suffering of their imprisonment.

Novel UEP LT Coding Scheme with Feedback Based on Different Degree Distributions

Directory of Open Access Journals (Sweden)

Li Ya-Fang

2016-01-01

Full Text Available Traditional unequal error protection (UEP schemes have some limitations and problems, such as the poor UEP performance of high priority data and the seriously sacrifice of low priority data in decoding property. Based on the reasonable applications of different degree distributions in LT codes, this paper puts forward a novel UEP LT coding scheme with a simple feedback to compile these data packets separately. Simulation results show that the proposed scheme can effectively protect high priority data, and improve the transmission efficiency of low priority data from 2.9% to 22.3%. Furthermore, it is fairly suitable to apply this novel scheme to multicast and broadcast environments since only a simple feedback introduced.

USAF Logistics Process Optimization Study for the Aircraft Asset Sustainment Process. Volume 3. Future to be Asset Sustainment Process Model

National Research Council Canada - National Science Library

Adamson, Anthony

1998-01-01

.... It is published as three separate volumes. Volume I, USAF Logistics Process Optimization Study for the Aircraft Asset Sustainment Process -- Phase II Report, discusses the result and cost/benefit analysis of testing three initiatives...

Characterization of LT-LiXO1-YNIYO2 electrodes for rechargeable lithium cells

CSIR Research Space (South Africa)

Gummow, RJ

1993-12-01

Full Text Available -spinel in character and that LT- Li0.4Co0.sNi0.102 is a defect spinel with spinel notation {Li0.s\\[:\\]0.2}sa \\[Co, 6Nio.2D0.2104. Electrochemical data.--The charge and discharge pro- files for the first four cycles of Li/LT-LiCoO2, Li... on the B sites of an A\\[B2104 spinel structure. The spinel phase is significantly more stable to lithium insertion/extraction reactions than the quasi-spinel phase. It is believed that by optimizing the processing conditions...

Nanoscale characterization and local piezoelectric properties of lead-free KNN-LT-LS thin films

Energy Technology Data Exchange (ETDEWEB)

Abazari, M; Safari, A [Glenn Howatt Electroceramics Laboratories, Department of Materials Science and Engineering, Rutgers-The state University of New Jersey, Piscataway, NJ 08854 (United States); Choi, T; Cheong, S-W [Rutgers Center for Emergent Materials, Department of Physics and Astronomy, Rutgers-The state University of New Jersey, Piscataway, NJ 08854 (United States)

2010-01-20

We report the observation of domain structure and piezoelectric properties of pure and Mn-doped (K{sub 0.44},Na{sub 0.52},Li{sub 0.04})(Nb{sub 0.84},Ta{sub 0.1},Sb{sub 0.06})O{sub 3} (KNN-LT-LS) thin films on SrTiO{sub 3} substrates. It is revealed that, using piezoresponse force microscopy, ferroelectric domain structure in such 500 nm thin films comprised of primarily 180{sup 0} domains. This was in accordance with the tetragonal structure of the films, confirmed by relative permittivity measurements and x-ray diffraction patterns. Effective piezoelectric coefficient (d{sub 33}) of the films were calculated using piezoelectric displacement curves and shown to be {approx}53 pm V{sup -1} for pure KNN-LT-LS thin films. This value is among the highest values reported for an epitaxial lead-free thin film and shows a great potential for KNN-LT-LS to serve as an alternative to PZT thin films in future applications.

Nanoscale characterization and local piezoelectric properties of lead-free KNN-LT-LS thin films

Science.gov (United States)

Abazari, M.; Choi, T.; Cheong, S.-W.; Safari, A.

2010-01-01

We report the observation of domain structure and piezoelectric properties of pure and Mn-doped (K0.44,Na0.52,Li0.04)(Nb0.84,Ta0.1,Sb0.06)O3 (KNN-LT-LS) thin films on SrTiO3 substrates. It is revealed that, using piezoresponse force microscopy, ferroelectric domain structure in such 500 nm thin films comprised of primarily 180° domains. This was in accordance with the tetragonal structure of the films, confirmed by relative permittivity measurements and x-ray diffraction patterns. Effective piezoelectric coefficient (d33) of the films were calculated using piezoelectric displacement curves and shown to be ~53 pm V-1 for pure KNN-LT-LS thin films. This value is among the highest values reported for an epitaxial lead-free thin film and shows a great potential for KNN-LT-LS to serve as an alternative to PZT thin films in future applications.

In Vitro Toxicity of Aluminum Nanoparticles in Rat Alveolar Macrophages

Science.gov (United States)

2006-03-01

including intravenous, intramuscular , and subcutaneous injections, and including oral and ocular administration (Kreuter, 1991). NPs allow delivery of... NANOPARTICLES IN RAT ALVEOLAR MACROPHAGES THESIS Andrew J Wagner, 1st Lt, USAF AFIT/GES/ENV/06M-06 DEPARTMENT OF THE AIR FORCE AIR UNIVERSITY ORCE...TOXICITY OF ALUMINUM NANOPARTICLES IN RAT ALVEOLAR MACROPHAGES THESIS Presented to the Faculty Department of Systems and Engineering

A Gene Gun-mediated Nonviral RNA trans-splicing Strategy for Col7a1 Repair

Directory of Open Access Journals (Sweden)

Patricia Peking

2016-01-01

Full Text Available RNA trans-splicing represents an auspicious option for the correction of genetic mutations at RNA level. Mutations within COL7A1 causing strong reduction or absence of type VII collagen are associated with the severe skin blistering disease dystrophic epidermolysis bullosa. The human COL7A1 mRNA constitutes a suitable target for this RNA therapy approach, as only a portion of the almost 9 kb transcript has to be delivered into the target cells. Here, we have proven the feasibility of 5′ trans-splicing into the Col7a1 mRNA in vitro and in vivo. We designed a 5′ RNA trans-splicing molecule, capable of replacing Col7a1 exons 1–15 and verified it in a fluorescence-based trans-splicing model system. Specific and efficient Col7a1 trans-splicing was confirmed in murine keratinocytes. To analyze trans-splicing in vivo, we used gene gun delivery of a minicircle expressing a FLAG-tagged 5′ RNA trans-splicing molecule into the skin of wild-type mice. Histological and immunofluorescence analysis of bombarded skin sections revealed vector delivery and expression within dermis and epidermis. Furthermore, we have detected trans-spliced type VII collagen protein using FLAG-tag antibodies. In conclusion, we describe a novel in vivo nonviral RNA therapy approach to restore type VII collagen expression for causative treatment of dystrophic epidermolysis bullosa.

Spring 2008 Industry Study. Privatized Military Operations Industry

Science.gov (United States)

2008-01-01

Department of Defense Civilian Lt Col Nathan “ Chili ” Lindsay, United States Air Force COL Darius Lukowski, Polish Army Ms. Diane Meyer, Department...efforts of the USG. Recommendations This Liberia case study demonstrates the benefits and costs of using contractors for the execution of U.S...PMFs and the USG to explore innovative ways to partner such that both parties would benefit by identifying requirements and capabilities in a more

Comparison of Maxilla Mandibular Transverse Ratios With Class II Anteroposterior Discrepancies

Science.gov (United States)

2014-03-20

skeletal relationships. Malocclusion can occur in all three planes of space and result from dental , skeletal, or both dental and skeletal anomalies ...somewhat surprising when considering the prevalence of dental anomalies such as root dilacerations and morphologic anomalies . The data suggests that...Approval: \\SCJ o------===== Date 10Jul14 SERVICE DEAN APPROVAL 1. Name: DREW W. FALUS, COL, USAF. DC DEAN, GRADUATE DENTAL EDUCATION 2. School (if

Leadership training in Endocrinology fellowship A survey of program directors and recent graduates

Science.gov (United States)

2017-06-16

Program Directors and Recent Graduates presented at/ published to SAMHS and Universities Research Forum (SURF) 2017, San Antonio, TX 16 June 2017 m...Research Division may pay for your basic journal publishing charges (to include costs for tables and black and white photos). We cannot pay for...efforts. LINDA STEEL-GOODWIN, Col, USAF, BSC Director , Clinical Investigations & Research Support Warrior Medics - Mission Ready - Patient Focused

Human LT-alpha-mediated resistance to autoimmune diabetes is induced in NOD, but not NOD-scid, mice and abrogated by IL-12.

Science.gov (United States)

Miyaguchi, S; Satoh, J; Takahashi, K; Sakata, Y; Nakazawa, T; Miyazaki, J; Toyota, T

2001-01-01

Systemic administration of human lymphotoxin-alpha (hLT-alpha) made NOD mice resistant not only to spontaneous autoimmune type 1 diabetes mellitus but also to cyclophosphamide (CY)-induced diabetes and diabetes transfer by diabetic NOD spleen cells (triple resistance). In this study we analyzed the mechanisms of hLT-alpha-induced resistance, focusing on (1) hLT-alpha-induced resistance in the pancreatic beta cell, (2) CY-resistant suppressor cells, (3) suppression of induction or function of effector cells for beta cell destruction, or (4) others. To examine the first possibility in vitro, a NOD-derived beta cell line (MIN6N) was pretreated with hLT-alpha and then mixed with diabetic NOD spleen cells and MIN6N cell viability was measured. Treatment with hLT-alpha did not protect MIN6N cells but rather enhanced cytotoxicity. Next NOD-scid mice were pretreated with hLT-alpha and then transferred with diabetic NOD spleen. All the recipients developed diabetes. These results excluded the first possibility. The second possibility was also excluded by a cotransfer experiment, in which diabetic NOD spleen cells were cotransferred to NOD-scid mice with nontreated or hLT-alpha-treated nondiabetic NOD spleens. There was no significant difference in diabetes incidence between the two groups. To observe the third possibility, spleen cells of hLT-alpha-treated triple-resistant NOD mice were transferred to NOD-scid mice. Diabetes developed in the recipients, although the onset of diabetes was slightly delayed. Finally, hLT-alpha-treated triple-resistant NOD mice developed diabetes 1 week after daily IL-12 treatment. In summary, hLT-alpha administration made NOD mice resistant to effector cells for beta cell destruction. This resistance was induced in NOD, but not in NOD-scid, mice, indicating that lymphocytes were obligatory for the resistance. However, it was not mediated by transferable suppressor cells. Because effector cells were present in hLT-alpha-treated NOD spleen and

AutoCAD / AutoCAD LT 2014 fundamentals metric

CERN Document Server

ASCENT center for technical knowledge

2014-01-01

The objective of AutoCAD/AutoCAD LT 2014 Fundamentals is to enable students to create a basic 2D drawing in the AutoCAD software. Even at this fundamental level, the AutoCAD software is one of the most sophisticated computer applications that you are likely to encounter. Therefore learning to use it can be challenging. To make the process easier and provide flexibility for instructors and students, the training guide is divided into two parts that can be taken independently.

LT-CFB. Further development and commercialization. Final report. [Low Temperature Circulating Fluid Bed gasifier]; LT-CFB. Videreudvikling og kommercialisering. Slutrapport

Energy Technology Data Exchange (ETDEWEB)

Stoholm, P. [DFBT, Roskilde (Denmark); Birk Henriksen, U.; Ahrentfeldt, J. [Technical Univ. of Denmark. DTU Chemical Engineering, CHEC Research Centre, Roskilde (Denmark); Cramer, J.; Dietrich, J.; Christiansen, Knud [FORCE Technology, Kgs. Lyngby (Denmark); Krogh, J. [Anhydro A/S, Soeborg (Denmark)

2011-12-15

The starting point for the project was the good results achieved in a previous project, in which a series of experiments were carried out with a 500 kW pilot plant at teh Technical University of Denmark. The main task was then to find a way forward towards further scale-up, demonstration and commercialization. The project's partners chose to study three possible sites for demonstration. However, during the project both framework conditions as well as DONG Energy's strategy changed, which resulted in the company taking over the LT-CFB technology in December 2009. As a first step a 6 MW demonstration plant will now be set up at the Asnaes power plant expected to start in spring 2011. The project has also been buit a new mobile 100 kW LT-CFB plant for the further optimization of the process and for short-term trials of new fuels. At the new plant a number of successful trials were performed with straw and residual fibers. Furthermore, it is experimentally demonstrated that it is possible to cool the tar-containing gas to approx. 300 degrees C and purify the gas in a bag filter, so it can be used in natural gas-fired power plant boilers. With the new mobile system, it will now be easier to perform experiments with a number of new fuels such as unsorted municipal waste, bone meal and dried sewage sludge. (LN)

USAF Flight Surgeon Survey: Aircrew Mental Health Referrals and Satisfaction with Local Mental Health Providers Response

Science.gov (United States)

2008-05-01

Autogenic training exercise; A treatment for airsickness in military pilots. International Journal of Aviation Psychology, 2005; 15(4): 395-412...flying during training , humanitarian, and operational missions can be extremely taxing. Flight surgeons often observe or hear of changes in the...health care is to ease and resolve the emotional or behavioral difficulties of an aviator while attempting to preserve a highly trained USAF asset

Linkage mapping of the gene for Type III collagen (COL3A1) to human chromosome 2q using a VNTR polymorphism

Energy Technology Data Exchange (ETDEWEB)

Tiller, G.E.; Polumbo, P.A.; Summar, M.L. (Vanderbilt Univ. Medical Center, Nashville, TN (United States))

1994-03-15

The gene for the [alpha]1(III) chain of type III collagen, COL3A1, has been previously mapped to human chromosome 2q24.3-q31 by in situ hybridization. Physical mapping by pulsed-field gel electrophoresis has demonstrated that COL3A1 lies within 35 kb of COL5A2. The authors genotyped the CEPH families at the COL3A2 locus using a pentanucleotide repeat polymorphism within intron 25. They demonstrated significant linkage to 18 anonymous markers as well as the gene for carbamyl phosphate synthetase (CPSI), which had been previously mapped to this region. No recombination was seen between COL3A1 and COL5A2 (Z = 9.93 at [theta] = 0) or D2S24 (Z = 10.55 at [theta] = 0). The locus order is (D2S32-D2S138-D2S148)-(D2S24-COL5A2-COL3A1)-(D2S118-D2S161), with odds of 1:2300 for the next most likely order. These relationships are consistent with the physical mapping of COL3A1 to the distal portion of 2q and place it proximal to CPSI by means of multipoint analysis. These linkage relationships should prove useful in further studies of Ehlers-Danlos syndrome type IV and carbamyl phosphate synthetase I deficiency and provide an additional framework for localizing other genes in this region. 13 refs., 2 figs., 1 tab.

Compilation of Abstracts of Theses Submitted by Candidates for Degrees, 1 October 1981 - 30 September 1982.

Science.gov (United States)

1983-05-01

Interactive Program to 238 LT, USCG Design Supercavitating PropellerBlades METEOROLOGY 239 Conant, P. R. A Study of East-Coast Cyclogenesis 241 CAPT, USAF...Engineering 237 • -- . -- -.. .-.. . .. ,- ---.-"-. • - , , - A SIMPLE INTERACTIVE PROGRAM TO DESIGN SUPERCAVITATING PROPELLER BLADES Marc B. Wilson...desk top computer (i.e.. HP 9845) was developed for a first cut approximation for the design of a supercavitating propeller blade. This program elimi

Russian Perspectives on Network-Centric Warfare: The Key Aim of Serdyukov’s Reform

Science.gov (United States)

2011-01-01

has faced with its GLObal’naya NAvigatsionnaya Sputnikovaya Sistema —(GLONASS) system, and introducing advanced digitized communications in the armed...of targeting data will be accomplished by organizing virtual channels for data transmission or via a web -portal. The successful function- ing of the...Operations: Tactical Web Takes Shape,” Signal, November 2003; Col. Alan D. Campen, USAF (Ret.), “Look Closely At Network-Centric Warfare: Technology Can

Novel COL4A1 mutations associated with HANAC syndrome: a role for the triple helical CB3[IV] domain.

Science.gov (United States)

Plaisier, Emmanuelle; Chen, Zhiyong; Gekeler, Florian; Benhassine, Safa; Dahan, Karine; Marro, Béatrice; Alamowitch, Sonia; Paques, Michel; Ronco, Pierre

2010-10-01

The COL4A1 gene encodes the α1-chain of type IV collagen, which is ubiquitously expressed in basement membranes. Mutations in COL4A1 have been reported in autosomal-dominant porencephaly and in patients with symptomatic small vessel brain disease, inconstantly associated with eye defects. We have previously reported three COL4A1 mutations associated with a systemic phenotype that we called HANAC (Hereditary Angiopathy, Nephropathy, Aneurysms, and Cramps). We carried out a clinical and genetic study of three families presenting with characteristic features of HANAC syndrome. Common systemic signs included arterial retinal tortuosity and muscle cramps, with a variable combination of small vessel brain disease, Raynaud phenomena, and kidney defects. Three novel COL4A1 missense substitutions are described, which affect highly conserved glycine residues within the collagenous domain of the protein. All six known mutations associated with the HANAC phenotype are localized within the CB3[IV] fragment of COL4A1, which encompasses major integrin-binding sites. Our results confirm that HANAC syndrome is a distinct clinical entity within the COL4A1-related disorders, which is characterized by systemic involvement and usually asymptomatic brain disease. The restricted distribution of COL4A1 mutations within the CB3[IV] region is a characteristic of the reports of patients with HANAC, which suggests that abnormal cell-type IV collagen interactions may underlie the systemic defects observed in this syndrome. Copyright © 2010 Wiley-Liss, Inc.

Tissue-specific mosaicism for a lethal osteogenesis imperfecta COL1A1 mutation causes mild OI/EDS overlap syndrome.

Science.gov (United States)

Symoens, Sofie; Steyaert, Wouter; Demuynck, Lynn; De Paepe, Anne; Diderich, Karin E M; Malfait, Fransiska; Coucke, Paul J

2017-04-01

Type I collagen is the predominant protein of connective tissues such as skin and bone. Mutations in the type I collagen genes (COL1A1 and COL1A2) mainly cause osteogenesis imperfecta (OI). We describe a patient with clinical signs of Ehlers-Danlos syndrome (EDS), including fragile skin, easy bruising, recurrent luxations, and fractures resembling mild OI. Biochemical collagen analysis of the patients' dermal fibroblasts showed faint overmodification of the type I collagen bands, a finding specific for structural defects in type I collagen. Bidirectional Sanger sequencing detected an in-frame deletion in exon 44 of COL1A1 (c.3150_3158del), resulting in the deletion of three amino acids (p.Ala1053_Gly1055del) in the collagen triple helix. This COL1A1 mutation was hitherto identified in four probands with lethal OI, and never in EDS patients. As the peaks on the electropherogram corresponding to the mutant allele were decreased in intensity, we performed next generation sequencing of COL1A1 to study mosaicism in skin and blood. While approximately 9% of the reads originating from fibroblast gDNA harbored the COL1A1 deletion, the deletion was not detected in gDNA from blood. Most likely, the mild clinical symptoms observed in our patient can be explained by the mosaic state of the mutation. © 2017 Wiley Periodicals, Inc.

Effects of a malfunctional column on conventional and FeedCol-simulated moving bed chromatography performance.

Science.gov (United States)

Song, Ji-Yeon; Oh, Donghoon; Lee, Chang-Ha

2015-07-17

The effects of a malfunctional column on the performance of a simulated moving bed (SMB) process were studied experimentally and theoretically. The experimental results of conventional four-zone SMB (2-2-2-2 configuration) and FeedCol operation (2-2-2-2 configuration with one feed column) with one malfunctional column were compared with simulation results of the corresponding SMB processes with a normal column configuration. The malfunctional column in SMB processes significantly deteriorated raffinate purity. However, the extract purity was equivalent or slightly improved compared with the corresponding normal SMB operation because the complete separation zone of the malfunctional column moved to a lower flow rate range in zones II and III. With the malfunctional column configuration, FeedCol operation gave better experimental performance (up to 7%) than conventional SMB operation because controlling product purity with FeedCol operation was more flexible through the use of two additional operating variables, injection time and injection length. Thus, compared with conventional SMB separation, extract with equivalent or slightly better purity could be produced from FeedCol operation even with a malfunctional column, while minimizing the decrease in raffinate purity (less than 2%). Copyright © 2015 Elsevier B.V. All rights reserved.

A novel COL11A1 missense mutation in siblings with non-ocular Stickler syndrome.

Science.gov (United States)

Kohmoto, Tomohiro; Tsuji, Atsumi; Morita, Kei-Ichi; Naruto, Takuya; Masuda, Kiyoshi; Kashimada, Kenichi; Enomoto, Keisuke; Morio, Tomohiro; Harada, Hiroyuki; Imoto, Issei

2016-01-01

Stickler syndrome (STL) is an autosomal, dominantly inherited, clinically variable and genetically heterogeneous connective tissue disorder characterized by ocular, auditory, orofacial and skeletal abnormalities. We conducted targeted resequencing using a next-generation sequencer for molecular diagnosis of a 2-year-old girl who was clinically suspected of having STL with Pierre Robin sequence. We detected a novel heterozygous missense mutation, NM_001854.3:n.4838G>A [NM_001854.3 (COL11A1_v001):c.4520G>A], in COL11A1, resulting in a Gly to Asp substitution at position 1507 [NM_001854.3(COL11A1_i001)] within one of the collagen-like domains of the triple helical region. The same mutation was detected in her 4-year-old brother with cleft palate and high-frequency sensorineural hearing loss.

EuroCirCol - Horizon 2020 Research and Innovation Action

CERN Document Server

AUTHOR|(CDS)2108815; Benedikt, Michael; Zimmermann, Frank; Schulte, Daniel; Tommasini, Davide; Seryi, Andrei; Perez, Francis

2015-01-01

EuroCirCol is a conceptual design study for a post-LHC research infrastructure based on an energy-frontier 100 TeV circular hadron collider. ​The award of the 2013 Nobel Prize for Physics acknowledged the leading role of Europe in particle physics, which has a global community of over 10'000 scientists. To reinforce its pole position throughout the 21st century, Europe must be ready to propose an ambitious post-LHC accelerator project by 2018/19. This is one of the main recommendations of the updated European Strategy for Particle Physics, adopted by the CERN Council in May 2013. The EuroCirCol conceptual design study is a direct response to this recommendation, carried out through a collaboration of institutes and universities worldwide. A new research infrastructure of such scale depends on the feasibility of key technologies pushed beyond current state of the art. Innovative designs for accelerator magnets to achieve high-quality fields up to 16 T and for a cryogenic beam vacuum system to cope with unpre...

Evaluation of a patient with classical Ehlers-Danlos syndrome due to a 9q34 duplication affecting COL5A1.

Science.gov (United States)

Kuroda, Yukiko; Ohashi, Ikuko; Naruto, Takuya; Ida, Kazumi; Enomoto, Yumi; Saito, Toshiyuki; Nagai, Jun-Ichi; Kurosawa, Kenji

2018-03-09

Ehlers-Danlos syndrome classical type is a connective tissue disorder characterized by skin hyperextensibility, atrophic scarring, and joint hypermobility. The condition typically results from mutations in COL5A1 or COL5A2 leading to the functional haploinsufficiency. Here, we report of a 24-year-old male with mild intellectual disability, dysmorphic features, and a phenotype consistent with Ehlers-Danlos syndrome classical type. A copy number variant-calling algorithm from panel sequencing data identified the deletions exons 2-11 and duplications of exons 12-67 within COL5A1. Array comparative genomic hybridization confirmed a 94 kb deletion at 9q34.3 involving exons 2-11 of COL5A1, and a 3.4 Mb duplication at 9q34.3 involving exons 12-67 of COL5A1. © 2018 Japanese Teratology Society.

Contempt-LT: a computer program for predicting containment pressure-temperature response to a loss-of-coolant accident

International Nuclear Information System (INIS)

Wheat, L.L.; Wagner, R.J.; Niederauer, G.F.; Obenchain, C.F.

1975-06-01

CONTEMPT-LT is a digital computer program, written in FORTRAN IV, developed to describe the long-term behavior of water-cooled nuclear reactor containment systems subjected to postulated loss-of-coolant accident (LOCA) conditions. The program calculates the time variation of compartment pressures, temperatures, mass and energy inventories, heat structure temperature distributions, and energy exchange with adjacent compartments. The program is capable of describing the effects of leakage on containment response. Models are provided to describe fan cooler and cooling spray engineered safety systems. Up to four compartments can be modeled with CONTEMPT-LT, and any compartment except the reactor system may have both a liquid pool region and an air-vapor atmosphere region above the pool. Each region is assumed to have a uniform temperature, but the temperatures of the two regions may be different. CONTEMPT-LT can be used to model all current boiling water reactor pressure suppression systems, including containments with either vertical or horizontal vent systems. CONTEMPT-LT can also be used to model pressurized water reactor dry containments, subatmospheric containments, and dual volume containments with an annulus region, and can be used to describe containment responses in experimental containment systems. The program user defines which compartments are used, specifies input mass and energy additions, defines heat structure and leakage systems, and describes the time advancement and output control. CONTEMPT-LT source decks are available in double precision extended-binary-coded-decimal-interchange-code (EBCDIC) versions. Sample problems have been run on the IBM360/75 computer. (U.S.)

Autodesk Inventor 2012 and Inventor LT 2012 Essentials

CERN Document Server

Tremblay, Thom

2011-01-01

Essential guide to learning Autodesk Inventor and Inventor LT The new Essentials books from Sybex are beautiful, task-based, full-color Autodesk Official Training Guides that help you get up to speed on Autodesk topics quickly and easily. Inventor Essentials thoroughly covers core features and functions of Autodesk's industry-leading 3D mechanical design software, teaching you what you need to become quickly productive with the software. By following the book's clear explanations, practical tutorials, and step-by-step exercises, you'll cover all the bases. Topics include drawing, modeling part

Loss of Col3a1, the gene for Ehlers-Danlos syndrome type IV, results in neocortical dyslamination.

Directory of Open Access Journals (Sweden)

Sung-Jin Jeong

Full Text Available It has recently been discovered that Collagen III, the encoded protein of the type IV Ehlers-Danlos Syndrome (EDS gene, is one of the major constituents of the pial basement membrane (BM and serves as the ligand for GPR56. Mutations in GPR56 cause a severe human brain malformation called bilateral frontoparietal polymicrogyria, in which neurons transmigrate through the BM causing severe mental retardation and frequent seizures. To further characterize the brain phenotype of Col3a1 knockout mice, we performed a detailed histological analysis. We observed a cobblestone-like cortical malformation, with BM breakdown and marginal zone heterotopias in Col3a1⁻/⁻ mouse brains. Surprisingly, the pial BM appeared intact at early stages of development but starting as early as embryonic day (E 11.5, prominent BM defects were observed and accompanied by neuronal overmigration. Although collagen III is expressed in meningeal fibroblasts (MFs, Col3a1⁻/⁻ MFs present no obvious defects. Furthermore, the expression and posttranslational modification of α-dystroglycan was undisturbed in Col3a1⁻/⁻ mice. Based on the previous finding that mutations in COL3A1 cause type IV EDS, our study indicates a possible common pathological pathway linking connective tissue diseases and brain malformations.

Hypothyroid Patients Encoding Combined MCT10 and DIO2 Gene Polymorphisms May Prefer L-T3 + L-T4 Combination Treatment - Data Using a Blind, Randomized, Clinical Study

DEFF Research Database (Denmark)

Carlé, Allan; Faber, Jens; Steffensen, Rudi

2017-01-01

on the cellular membrane transport-facilitating monocarboxylate transporter (MCT10) gene (rs17606253), and asked in which of the 2 treatment periods patients felt better (i.e., which treatment was preferred). RESULTS: 27 out of 45 patients (60%) preferred the combination therapy. Two polymorphisms (rs225014 (DIO2......, Thr92Ala) and rs17606253 (MCT10)) were combined yielding 3 groups: none vs. 1 of 2 vs. both SNPs present, and 42 vs. 63 vs. 100% of our patients in the 3 groups preferred the combined treatment (Jongheere-Terpstra trend test, p = 0.009). CONCLUSION: The present study indicates that the combination...... of polymorphisms in DIO2 (rs225014) and MCT10 (rs17606253) enhances hypothyroid patients' preference for L-T4 + L-T3 replacement therapy. In the future, combination therapy may be restricted or may be even recommended to individuals harbouring certain polymorphisms....

2011 JSOU and NDIA SO/LIC Division Essays

Science.gov (United States)

2011-07-01

Khakrez dataset with broad-brush strokes , we focus more specifically on the common linkages between the two dominant tribes: the Popolzai and Alikozai...consequently only one potential heat source for IR-guided MANPADS versus the AC-130’s four engines. Cost. The OA-X would also be considerably more...Col. Philip Cochran flew P-40s in North Africa and Lt. Col. John Alison flew them with the Flying Tigers in China.32 AFSOC should supplement its

Pertinencia de los postgrados para economistas en el Núcleo LUZ–COL

Directory of Open Access Journals (Sweden)

Ana Teresa Prieto Sánchez

2002-01-01

Full Text Available El presente artículo tiene como finalidad presentar los resultados obtenidos en un estudio de mercado, cuyo objetivo fue determinar la pertinencia de los postgrados para economistas en el Núcleo LUZ-COL. En dicho estudio se analizaron las expectativas, necesidades y percepciones de los usuarios potenciales del servicio de postgrado, considerando como usuarios potenciales (demanda a los entes empleadores de la COL, docentes de economía en el Núcleo, egresados del programa de economía en el Núcleo y estudiantes del último semestre de la carrera. Estos elementos sirvieron de base para conocer la pertinencia o no del postgrado. La metodología utilizada se fundamentó en una investigación descriptiva y cualitativa, en donde se utilizó el cuestionario como instrumento, para recabar la información primaria. Los resultados muestran una alta necesidad de los programas de postgrados para economistas y delimitan una demanda potencial considerable. El análisis permitió concluir que sí son pertinentes los postgrados para economistas en el Núcleo COL, en las áreas de gerencia de empresas, formulación y evaluación de proyectos, costos o finanzas; del tipo maestría o especialización profesional. En virtud de los resultados, se recomienda, desarrollar estrategias viables que permitan a los tomadores de decisión implementar el postgrado para economistas en el Núcleo.

El rol del professor en l’aprenentatge col·laboratiu mediat per ordinador

Directory of Open Access Journals (Sweden)

Ingrid Noguera-Fructuoso

2009-10-01

Full Text Available En aquest article es presenten els resultats d'una investigació sobre el canvi de rol que es produeix en el professorat en l'ús de les TIC com a eina per a l'aprenentatge col·laboratiu en educació primària i secundària. L'interès es focalitza en l'ús concret d'una eina dissenyada per a l'aprenentatge col·laboratiu, el BSCL, sota el model pedagògic de qüestionament progressiu. En primer lloc, es duu a terme una revisió teòrica. En segon lloc, s'exposen les preguntes d'investigació, la justificació d'aquesta, es descriu el disseny i l'enfocament metodològic escollit, l'estudi de casos. Els resultats obtinguts revelen que hi ha una tendència cap al canvi de rol en el professorat en l'ús del BSCL com a eina per a l'aprenentatge col·laboratiu així com l'eficàcia del model de qüestionament progressiu en la construcció de coneixement de l'alumnat.

Complete COL1A1 allele deletions in osteogenesis imperfecta

NARCIS (Netherlands)

van Dijk, Fleur S.; Huizer, Margriet; Kariminejad, Ariana; Marcelis, Carlo L.; Plomp, Astrid S.; Terhal, Paulien A.; Meijers-Heijboer, Hanne; Weiss, Marjan M.; van Rijn, Rick R.; Cobben, Jan M.; Pals, Gerard

Purpose: To identify a molecular genetic cause in patients with a clinical diagnosis of osteogenesis imperfecta (OI) type I/IV. Methods: The authors performed multiplex ligation-dependent probe amplification analysis of the COL1A1 gene in a group of 106 index patients. Results: In four families with

Complete COL1A1 allele deletions in osteogenesis imperfecta

NARCIS (Netherlands)

van Dijk, Fleur S.; Huizer, Margriet; Kariminejad, Ariana; Marcelis, Carlo L.; Plomp, Astrid S.; Terhal, Paulien A.; Meijers-Heijboer, Hanne; Weiss, Marjan M.; van Rijn, Rick R.; Cobben, Jan M.; Pals, Gerard

2010-01-01

To identify a molecular genetic cause in patients with a clinical diagnosis of osteogenesis imperfecta (OI) type I/IV. The authors performed multiplex ligation-dependent probe amplification analysis of the COL1A1 gene in a group of 106 index patients. In four families with mild osteogenesis

Cisto gigante de colédoco Giant choledochal cyst

Directory of Open Access Journals (Sweden)

Olival Cirilo Lucena da Fonseca-Neto

2007-12-01

Full Text Available INTRODUÇÃO: A doença cística das vias biliares é anomalia congênita que pode acometer as vias biliares intra e/ou extra-hepáticas. A extra-hepática inclui os cistos de colédoco e a intra-hepática é conhecida por Doença de Caroli. Os cistos de colédoco de tamanho gigante são muito raros. OBJETIVO: Relatar o caso de um cisto de colédoco de tamanho gigante em uma paciente feminina. RELATO DE CASO: Mulher de 19 anos foi admitida com história de icterícia e acolia fecal há sete dias. Referia dor epigástrica associada com ingestão de dieta rica em gordura. Nos antecedentes pessoais relatou dois episódios de icterícia, aos 8 e 14 anos, que progrediram espontaneamente. No exame físico apresentava icterícia (+3/+4 e uma massa palpável indolor em mesogástrio foram os únicos achados. A ultrassonografia demonstrou grande formação cística de paredes finas adjacente ao hepatocolédoco, pâncreas e rim direito que media 18,5 x 10,2 cm. A colangioressonância confirmou o grande cisto de colédoco e hepatojejunoanastomose em "Y" de Roux após excisão do cisto e colecistectomia foi realizada. A formação cística media 20 x 15,5 x 12,5 cm e com um volume médio de 1000 mL. A paciente encontra-se em acompanhamento ambulatorial sem alterações hepatobiliares após o sétimo mês da operação. CONCLUSÃO: O cisto de colédoco deve fazer parte do diagnóstico diferencial em pacientes adultos jovens com icterícia e massa palpável; no entanto, a diferenciação entre ele e neoplasia maligna deve ser pesquisada.BACKGROUND: Choledochal cyst represents a rare congenital anomaly, eventually associated with intra and extrahepatic biliary tract disorders. Extrahepatic diseases include choledochal cysts and congenital dilation of the lower intrahepatic bile duct is known as Caroli's disease. Giant choledochal cyst constitutes a very rare abnormality. AIM:To report a giant choledochal cyst in a female patient. CASE REPORT: A 19-year

Ion heating at the disruptive instability in the LT-3 Tokamak

International Nuclear Information System (INIS)

Bell, M.G.; Hutchinson, I.H.

1976-01-01

Measurements of the ion temperature and the toroidal current density and electric field during the disruptive instability in LT-3 are presented. Rapid ion heating and strong current inhibition have been observed. Fluctuation measurements suggest that these effects may be attributable to the excitation of ion cyclotron drift waves in the plasma

Genetics Home Reference: COL4A1-related brain small-vessel disease

Science.gov (United States)

... hemorrhage Johns Hopkins Medicine Department of Neurology and Neurosurgery: Intracerebral Hemorrhage Johns Hopkins Medicine Department of Neurology and Neurosurgery: Stroke MalaCards: col4a1-related brain small-vessel disease ...

Stimulation of Escherichia coli F-18Col- Type-1 fimbriae synthesis by leuX

DEFF Research Database (Denmark)

Newman, Joseph V.; Burghoff, Robert L.; Pallesen, Lars

1994-01-01

Escherichia coli F-18, a normal human fecal isolate, is an excellent colonizer of the streptomycin-treated mouse large intestine. E. coli F-18Col-, a derivative of E. coli F-18 which no longer makes the E. coli F-18 colicin, colonizes the large intestine as well as E. coli F-18 when fed to mice...... alone but is eliminated when fed together with E. coli F-18. Recently we randomly cloned E. coli F-18 DNA into E. coli F-18Col- and let the mouse intestine select the best colonizer. In this way, we isolated a 6.5-kb E. coli F-18 DNA sequence that simultaneously stimulated synthesis of type 1 fimbriae...... and enhanced E. coli F-18Col- colonizing ability. In the present investigation we show that the gene responsible for stimulation of type 1 fimbriae synthesis appears to be leuX, which encodes a tRNA specific for the rare leucine codon UUG. Moreover, it appears that expression of leuX may be regulated by two...

Air and Space Power Journal. Volume 30, Number 3, Fall 2016

Science.gov (United States)

2016-01-01

industrial controller, and the processors on a modern car are all part of cyberspace, although only some of them are routinely connected to the Internet...pdf. Col Tom Torkelson, USAF Colonel Torkelson (USAFA; MA, University of Oklahoma; MS, Air Force Institute of Tech - nology; MAAS, Air University... 2019 , the F-35 will have be- come fully operational and a capable replacement, working alongside legacy air- craft (like the F-16) to conduct CAS in

LT^2C^2: A language of thought with Turing-computable Kolmogorov complexity

Directory of Open Access Journals (Sweden)

Santiago Figueira

2013-03-01

Full Text Available In this paper, we present a theoretical effort to connect the theory of program size to psychology by implementing a concrete language of thought with Turing-computable Kolmogorov complexity (LT^2C^2 satisfying the following requirements: 1 to be simple enough so that the complexity of any given finite binary sequence can be computed, 2 to be based on tangible operations of human reasoning (printing, repeating,. . . , 3 to be sufficiently powerful to generate all possible sequences but not too powerful as to identify regularities which would be invisible to humans. We first formalize LT^2C^2, giving its syntax and semantics, and defining an adequate notion of program size. Our setting leads to a Kolmogorov complexity function relative to LT^2C^2 which is computable in polynomial time, and it also induces a prediction algorithm in the spirit of Solomonoff’s inductive inference theory. We then prove the efficacy of this language by investigating regularities in strings produced by participants attempting to generate random strings. Participants had a profound understanding of randomness and hence avoided typical misconceptions such as exaggerating the number of alternations. We reasoned that remaining regularities would express the algorithmic nature of human thoughts, revealed in the form of specific patterns. Kolmogorov complexity relative to LT^2C^2 passed three expected tests examined here: 1 human sequences were less complex than control PRNG sequences, 2 human sequences were not stationary showing decreasing values of complexity resulting from fatigue 3 each individual showed traces of algorithmic stability since fitting of partial data was more effective to predict subsequent data than average fits. This work extends on previous efforts to combine notions of Kolmogorov complexity theory and algorithmic information theory to psychology, by explicitly proposing a language which may describe the patterns of human thoughts.Received: 12

The Effect Of Supraphysiologic Blood Pressure on Traumatic Brain Injury and Proximal Tissue Beds During Resuscitative Balloon Occlusion of the Aorta and Variable Aortic Control in a Porcine Model (Sus scrofa) of Polytrauma.

Science.gov (United States)

2017-04-27

Control In A Porcine Model (Sus Scrofa) Of Polytrauma . PRINCIPAL INVESTIGATOR (PI) / TRAINING COORDINATOR (TC): Lt Col Timothy Williams DEPARTMENT...Mandatory) The Effect of REBOA, Partial Aortic Occlusion and Aggressive Blood Transfusion on Traumatic Brain Injury in a Swine Polytrauma Model

Wound healing genes and susceptibility to cutaneous leishmaniasis in Brazil: Role of COL1A1

Science.gov (United States)

Almeida, Lucas; Oliveira, Joyce; Guimarães, Luiz Henrique; Carvalho, Edgar M; Blackwell, Jenefer M

2015-01-01

Previous studies have demonstrated a role for wound healing genes in resolution of cutaneous lesions caused by Leishmania spp. in both mice and humans, including the gene FLI1 encoding Friend leukaemia virus integration 1. Reduction of Fli1 expression in mice has been shown to result in up-regulation of collagen type I alpha 1 (Col1a1) and alpha 2 (Col1a2) genes and, conversely, in down-regulation of the matrix metalloproteinase 1 (Mmp1) gene, suggesting that Fli1 suppression is involved in activation of the profibrotic gene program. Here we examined single nucleotide polymorphisms (SNPs) in these genes as risk factors for cutaneous (CL) and mucosal leishmaniasis (ML), and leishmaniasis per se, caused by L. braziliensis in humans. SNPs were genotyped in 168 nuclear families (250 CL; 87 ML cases) and replicated in 157 families (402 CL; 39 ML cases). Family-based association tests (FBAT) showed the strongest association between SNPs rs1061237 (combined P=0.002) and rs2586488 (combined P=0.027) at COL1A1 and CL disease. This contributes to our further understanding of the role of wound healing in the resolution of CL disease, providing potential for therapies modulating COL1A1 via drugs acting on FLI1. PMID:25562121

PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samples Reveals Their Importance as Gene Capture Platforms

OpenAIRE

Manuel Ares-Arroyo; Cristina Bernabe-Balas; Alfonso Santos-Lopez; Maria R. Baquero; Kashi N. Prasad; Dolores Cid; Carmen Martin-Espada; Alvaro San Millan; Bruno Gonzalez-Zorn

2018-01-01

ColE1 plasmids are important vehicles for the spread of antibiotic resistance in the Enterobacteriaceae and Pasteurellaceae families of bacteria. Their monitoring is essential, as they harbor important resistant determinants in humans, animals and the environment. In this work, we have analyzed ColE1 replicons using bioinformatic and experimental approaches. First, we carried out a computational study examining the structure of different ColE1 plasmids deposited in databases. Bioinformatic an...

Association between the clinical classification of hypothyroidism and reduced TSH in LT4 supplemental replacement treatment for pregnancy in China.

Science.gov (United States)

Zhang, Lyu; Zhang, Zhaoyun; Ye, Hongying; Zhu, Xiaoming; Li, Yiming

2016-01-01

The study was aimed to evaluate the effects of levothyroxine (LT4) supplemental replacement treatment for pregnancy and analyze the associations between the clinical classification of hypothyroidism and reduced thyroid-stimulating hormone (TSH) in LT4 therapy. Totally, 195 pregnant women with hypothyroidism receiving routine prenatal care were enrolled. They were categorized into three groups: overt hypothyroidism (OH), subclinical hypothyroidism (SCH) with negative thyroperoxidase antibody (TPOAb), and SCH with positive TPOAb. The association between the clinical classification and reduced TSH in LT4 supplemental replacement treatment was assessed. The results indicated that reduced TSH was significantly different among the groups according to the clinical classifications (p = 0.043). The result was also significantly different between patients with OH and patients with SCH and negative TPOAb (p = 0.036). Similar result was reported for the comparison between patients with OH and patients with SCH and positive TPOAb (p = 0.016). Multiple variable analyses showed that LT4 supplementation, gestational age and the variable of clinical classifications were associated with reduced TSH independently. Our data suggested that the therapeutic effect of substitutive treatment with LT4 was significantly associated with different clinical classifications of hypothyroidism in pregnancy and the treatment should begin as soon as possible after diagnosis.

Receptores do factor estimulante de colónias de macrófagos do robalo

OpenAIRE

Oliveira, Márcio Adriano Guiomar de

2010-01-01

O receptor do factor estimulante de colónias de macrófagos, também conhecido como receptor do factor estimulante de colónias-1 (CSF1R), é um receptor de um factor de crescimento hematopoiético que é especificamente expresso em células do sistema fagocítico-mononuclear e desempenha um papel essencial no desenvolvimento e regulação destas células. O CSF1R já foi descrito em vários mamíferos e a sua biologia tem sido exaustivamente caracterizada nestes vertebrados mas o conheci...

The use of the laryngeal tube disposable (LT-D) by paramedics during out-of-hospital resuscitation-an observational study concerning ERC guidelines 2005.

Science.gov (United States)

Wiese, C H R; Semmel, T; Müller, J U; Bahr, J; Ocker, H; Graf, B M

2009-02-01

In the current guidelines of the European Resuscitation Council (ERC), tracheal intubation, as an instrument for securing the airway during resuscitation, has become less important for persons not trained in this method. For those persons, different supraglottic airway devices are recommended by the ERC. The present investigation deals with the application of the laryngeal tube disposable (LT-D) during pre-hospital resuscitation by paramedics. During a period of 2 years (2006-2008), we registered all cardiac arrest situations in which the LT-D had been applied according to the ERC guidelines 2005. Therefore, we investigated one emergency medical system in Germany. During the defined period, 92 resuscitation attempts, recorded on standardised data sheets, were included. The LT-D was used in 46% of all cardiac arrest situations. Overall, the LT-D was successfully inserted in more than 90% of all cases on first attempt. In 95% of all cases, no problems concerning ventilation of the patient were described. As an alternative airway device recommended by the ERC in 2005, the LT-D may enable airway control rapidly and effectively. Additionally, by using the LT-D, a reduced "no-flow-time" and a better outcome may be possible.

Yoğurt Yapımında Saf Kültürün Kullanımı

Directory of Open Access Journals (Sweden)

Sevda Kılıç

2015-02-01

Full Text Available Yoğurt, sütün L. bulgaricus ve S. thermophilus bakterilerini belirli oranlarda içeren kültürle aşılanması sonucu elde edilen bir süt mamülüdür. Yoğurt teknolojisinde yaklaşık 70 yıldır kültür kullanılmaktadır. Kültürün hazırlanmasında yararlanılan bakteriler, belirli özellikleri dikkate alınarak seçilmektedir. Daha sonra yağsız süt tozundan hazırlanan süte aşılanarak 42-44 oC’de inkübasyona bırakılmakta ve pH 4.7 olduğunda inkübasyona son verilerek çoğaltımı ve aktifleşmeleri sağlanmaktadır. Günümüzde diğer ülkelerde olduğu gibi ülkemizde de kültür kullanımı gıda maddeleri tüzüğünün 51. maddesine göre zorunlu tutulmaktadır. Ancak buna rağmen kültür, tam anlamıyla bilinmemekte veya bilinçli olarak kullanılmamaktadır. Yoğurt teknolojisinde kültür, kullanımı ile daha kaliteli yoğurt yapılmaktadır. Uzun süre tat, aroma ve yapısı bozulmadan saklanabilmekte, raf ömrü uzamaktadır. Normal sertlikte olan yoğurtta asitlik istenilen düzeyde olduğu için su salmada görülmez.

C2 System Engineering for the Adversities of the Amazon Environment

Science.gov (United States)

2013-06-01

G. Pinto – Col Engineer Departamento de Ciência e Tecnologia (Science and Technology Department) agoncal@gmail.com Andersonn Kohl – Lt Col...Departamento de Ciência e Tecnologia , (2) Centro de Desenvolvimento de Sistemas, (3) 4º Centro de Telemática de Área, (4) Centro Tecnológico do...Integration. (pp. 14). Paper at 17 th ICCRTS [8] Guedes, A.R. & Leite, R.M.O, (2012) Estudo de Viabilidade Técnica e Econômica da Tecnologia HAP em

A Study to Determine Patient Waiting Time at the Outpatient Pharmacy at Wilford Hall USAF Medical Center

Science.gov (United States)

1988-06-01

at Wilford Hall USAF Medical Center significantly reduced the patient wait time at the main outpatient pharmacy. Satellite pharmacies have been ).’l...PRESENTING TO WINDOW 1, 19 MAR 88. 47 C:. A’.’E-:A: -ESCRIRTIONS PER PATIENT ...........48 H. WILFORD HALL MEDICAL CENTER OUTPATIENT QUESTIONNAIRE...that wait times at tne outpatient pharmacy were excessive. It was this concern that motivated the Medical Center Administrator to request that patient

PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samples Reveals Their Importance as Gene Capture Platforms

Directory of Open Access Journals (Sweden)

Manuel Ares-Arroyo

2018-03-01

Full Text Available ColE1 plasmids are important vehicles for the spread of antibiotic resistance in the Enterobacteriaceae and Pasteurellaceae families of bacteria. Their monitoring is essential, as they harbor important resistant determinants in humans, animals and the environment. In this work, we have analyzed ColE1 replicons using bioinformatic and experimental approaches. First, we carried out a computational study examining the structure of different ColE1 plasmids deposited in databases. Bioinformatic analysis of these ColE1 replicons revealed a mosaic genetic structure consisting of a host-adapted conserved region responsible for the housekeeping functions of the plasmid, and a variable region encoding a wide variety of genes, including multiple antibiotic resistance determinants. From this exhaustive computational analysis we developed a new PCR-based technique, targeting a specific sequence in the conserved region, for the screening, capture and sequencing of these small plasmids, either specific for Enterobacteriaceae or specific for Pasteurellaceae. To validate this PCR-based system, we tested various collections of isolates from both bacterial families, finding that ColE1 replicons were not only highly prevalent in antibiotic-resistant isolates, but also present in susceptible bacteria. In Pasteurellaceae, ColE1 plasmids carried almost exclusively antibiotic resistance genes. In Enterobacteriaceae, these plasmids encoded a large range of traits, including not only antibiotic resistance determinants, but also a wide variety of genes, showing the huge genetic plasticity of these small replicons. Finally, we also used a metagenomic approach in order to validate this technique, performing this PCR system using total DNA extractions from fecal samples from poultry, turkeys, pigs and humans. Using Illumina sequencing of the PCR products we identified a great diversity of genes encoded by ColE1 replicons, including different antibiotic resistance

Identification of a novel COL1A1 frameshift mutation, c.700delG, in a Chinese osteogenesis imperfecta family

Science.gov (United States)

Wang, Xiran; Pei, Yu; Dou, Jingtao; Lu, Juming; Li, Jian; Lv, Zhaohui

2015-01-01

Osteogenesis imperfecta (OI) is a family of genetic disorders associated with bone loss and fragility. Mutations associated with OI have been found in genes encoding the type I collagen chains. People with OI type I often produce insufficient α1-chain type I collagen because of frameshift, nonsense, or splice site mutations in COL1A1 or COL1A2. This report is of a Chinese daughter and mother who had both experienced two bone fractures. Because skeletal fragility is predominantly inherited, we focused on identifying mutations in COL1A1 and COL1A2 genes. A novel mutation in COL1A1, c.700delG, was detected by genomic DNA sequencing in the mother and daughter, but not in their relatives. The identification of this mutation led to the conclusion that they were affected by mild OI type I. Open reading frame analysis indicated that this frameshift mutation would truncate α1-chain type I collagen at residue p263 (p.E234KfsX264), while the wild-type protein would contain 1,464 residues. The clinical data were consistent with the patients’ diagnosis of mild OI type I caused by haploinsufficiency of α1-chain type I collagen. Combined with previous reports, identification of the novel mutation COL1A1-c.700delG in these patients suggests that additional genetic and environmental factors may influence the severity of OI. PMID:25983617

A PTEN-COL17A1 fusion gene and its novel regulatory role in Collagen XVII expression and GBM malignance.

Science.gov (United States)

Yan, Xiaoyan; Zhang, Chuanbao; Liang, Tingyu; Yang, Fan; Wang, Haoyuan; Wu, Fan; Wang, Wen; Wang, Zheng; Cheng, Wen; Xu, Jiangnan; Jiang, Tao; Chen, Jing; Ding, Yaozhong

2017-10-17

Collagen XVII expression has recently been demonstrated to be correlated with the tumor malignance. While Collagen XVII is known to be widely distributed in neurons of the human brain, its precise role in pathogenesis of glioblastoma multiforme (GBM) is unknown. In this study, we identified and characterized a new PTEN-COL17A1 fusion gene in GMB using transcriptome sequencing. Although fusion gene did not result in measurable fusion protein production, its presence is accompanied with high levels of COL17A1 expression, revealed a novel regulatory mechanism of Collagen XVII expression by PTEN-COL17A1 gene fusion. Knocked down Collagen XVII expression in glioma cell lines resulted in decreased tumor invasiveness, along with significant reduction of MMP9 expression, while increased Collagen XVII expression promotes invasive activities of glioma cells and associated with GBM recurrences. Together, our results uncovered a new PTEN-COL17A1 fusion gene and its novel regulatory role in Collagen XVII expression and GBM malignance, and demonstrated that COL17A1 could serve as a useful prognostic biomarker and therapeutic targets for GBM.

The Antiaircraft Journal. Volume 93, Number 6, November-December 1950

Science.gov (United States)

1950-12-01

compromise. Russia and the satellites deposited a reser- vation against freeing the original Detaining Power from responsibility. HUl\\ IANE TREATMENT OF...H., 543 Flynn Ave., Redwood City, Calif. Cox, Thomas R., 46 Maitland Rd., Stamford, Conn. DeRita, Joseph, Box 83, Lafayette, R. I. DiEnno, John, Jr... Ian 2-40 Broudy, Harold, Capt. 2- 2 Cunningham, Elmo E., 1st Lt. 5- 7 Currie, A. A., Lt. Col. 1-26 Dillon, Joseph V., Brig. Gen 6-34 Dineley, R. L., M

A Civilian/Military Trauma Institute: National Trauma Research Coordinating Center

Science.gov (United States)

2011-10-01

Rebuttals, Q&A Salon A Craniofacial Trauma LtCol Cecila Schmalbach 1540-1550 Speaker: Dr. Manuel Lopez Title: OIF: Perspective of H&N Surgeon in...Intubation Endoscope. Station 10 Video Laryngoscope Dr. (Maj) Elvin Cruz , Staff Anesthesiologist, Wilford Hall Medical Center Practice video

Mega, February 1941: The role of the 1st South African Irish Regiment

African Journals Online (AJOL)

1 Bn Natal Mounted Rifles (Lt Col N.D. McMillan). Field Force Brigade .... by Mr West, in the following extract from his memoirs4: ..... rian and Gold Coast battalions), in which the. European ...... they arrived near the bottom of the ridge which.

Mastering Autodesk Inventor 2014 and Autodesk Inventor LT 2014

CERN Document Server

Waguespack, Curtis

2013-01-01

An Autodesk Official Press guide to the powerful mechanical design software Autodesk Inventor has been used to design everything from cars and airplanes to appliances and furniture. This comprehensive guide to Inventor and Inventor LT features real-world workflows and work environments, and is packed with practical tutorials that focus on teaching Inventor tips, tricks, and techniques. Additionally, you can download datasets to jump in and practice on any exercise. This reference and tutorial explains key interface conventions, capabilities, tools, and techniques, including desi

BLM Communications Use Lease to USAF to Conduct Patriot Communications Exercises in Lincoln County, Nevada. Final Environmental Assessment

Science.gov (United States)

2008-08-01

Endangered Species Act of 1973, 1988 Amendments (16 USC § 1531 et seq.) FAA = Federal Aviation Authority FOC = Fiber Optic Cable FONSI...Disturbed Salt Desert Scrub The site was moderately grazed with denser vegetation and basalt rock type soil. Common species include blackbrush...volume, mild eruptions of basalt occurred in the region, resulting in basaltic cinder cones and lava flows (USAF, 1999). No Radar/Communications

Always an Airman: Enhancing the USAF Reserve’s Continuum of Service

Science.gov (United States)

2012-02-09

meet the needs of serving their nation, their families, and their civilian careers is paramount.”5 Lt Gen Charles Stenner Jr., the Chief of Air...Charles Stenner , Jr., Chief of Air Force Reserve, email to author, January 18, 2012. 8 Department of Defense, Managing the Reserve Components as an

Generation of Col2a1-EGFP iPS cells for monitoring chondrogenic differentiation.

Directory of Open Access Journals (Sweden)

Taku Saito

Full Text Available Induced pluripotent stem cells (iPSC are a promising cell source for cartilage regenerative medicine; however, the methods for chondrocyte induction from iPSC are currently developing and not yet sufficient for clinical application. Here, we report the establishment of a fluorescent indicator system for monitoring chondrogenic differentiation from iPSC to simplify screening for effective factors that induce chondrocytes from iPSC. We generated iPSC from embryonic fibroblasts of Col2a1-EGFP transgenic mice by retroviral transduction of Oct4, Sox2, Klf4, and c-Myc. Among the 30 clones of Col2a1-EGFP iPSC we established, two clones showed high expression levels of embryonic stem cell (ESC marker genes, similar to control ESC. A teratoma formation assay showed that the two clones were pluripotent and differentiated into cell types from all three germ layers. The fluorescent signal was observed during chondrogenic differentiation of the two clones concomitant with the increase in chondrocyte marker expression. In conclusion, Col2a1-EGFP iPSC are useful for monitoring chondrogenic differentiation and will contribute to research in cartilage regenerative medicine.

The Akashi Kaikyo Bridge and the Storebælt Bridge

DEFF Research Database (Denmark)

Gimsing, Niels Jørgen

1999-01-01

With the completion of the Akashi Kaikyo Bridge and the Storebælt East Bridge the development of the suspension bridge technology in the 20th century has manifested itself in two impressive structures. With the present echnology may bridges of similar (and also more modest) dimensions...... will undoubtedly be built far into the next century. For bridges going beyond the spans of existing bridges it is, however, likely that new concepts will be developed....

Joint Force Quarterly. Issue 67, 4th Quarter, October 2012

Science.gov (United States)

2012-10-01

Future of Cyber War ,” Survival 53, no. 1 [January 2011], 24.). 43 According to reports, representatives from the International Atomic Energy Agency who...Braden Hisey, and Jessica Todd 63 Space and the Joint Fight By Robert L. Butterworth 71 The Regional Special Operations Headquarters: Franchising the...and Resource Strategy RADM John N. Christenson, USN Naval War College Brig Gen Stephen T. Denker, USAF Air Command and Staff College LtGen George

Biotech Business Lessons for Defense Acquisition

Science.gov (United States)

2016-01-01

repudiates what func- tional personnel hold dear—that their hurdles are vital to some moral or ethical responsibility to protect or safeguard...94 Strategic Studies Quarterly ♦ Summer 2016 Biotech Business Lessons for Defense Acquisition Col David L. Peeler Jr., USAF Abstract The desire to...examples and lessons from private business sectors would serve DOD interests well. ✵ ✵ ✵ ✵ ✵ Acquisition reform! No other two words so stress or trouble

Deliberate Force. A Case Study in Effective Air Campaigning

Science.gov (United States)

2000-01-01

1995); Laura Silber and Allan Little, Yugoslavia: Death of a Nation (New York: TV Books/Penguin USA, 1996); Miron Rezun, Europe and War in the Balkans...takeoff to recovery back at Aviano and then finally carried through with the actual missions. As a result of the increasing number of units tasked to...went a renaissance. Col John R. Baker from Headquarters USAF, Director of Operations, led a US joint- service assess- ment team to the CAOC on 24

Autodesk Inventor 2013 and Autodesk Inventor LT 2013 essentials

CERN Document Server

Tremblay, Thom

2012-01-01

Get up to speed with Autodesk Inventor, the leading manufacturing design program This Autodesk Official Training Guide thoroughly covers the fundamentals of Autodesk Inventor 2013 and Inventor LT 2013. Focusing on basics such as using the interface, creating parts and assemblies, applying standards and styles, creating 2D drawings from 3D data, and more, it teaches you everything you need to become quickly productive with the software. Whether you're a new student learning CAD, preparing for certification, or updating your Inventor skills, this is the fast, thorough grounding you need. Featu

A new avian fauna from the early-middle Eocene Lillebælt Clay Formation of Denmark

DEFF Research Database (Denmark)

Lindow, Bent Erik Kramer

A number of hitherto undescribed fossil bird remains have been recovered from the Lillebælt Clay Formation of central Denmark, which is early-middle Eocene in age (~50 to 43 mya). The core of the material consists of fossils acquired through the Danish ‘Danekræ' fossil treasure trove legislation......, a member of the extinct 'pseudo-toothed birds' and the first representative of this group known from Denmark. Other taxa present include remains of Lithornithidae and a new taxon possessing a massive, psittacid-like beak. The Lillebælt Clay Formation birds are temporally placed just after the Early Eocene...

Düşey Toplum Kuramı Yaklaşımıyla Japon Çalışma Kültürünü Anlamak

OpenAIRE

Aca, Zeynep

2015-01-01

Japon çalışma kültürünün temel felsefesini anlayabilmenin yolu, Japon toplumunun kültürel özelliklerini anlayabilmekten geçmektedir. Ancak kültürü oluşturan her bir özellik elbetteki Japon çalışma kültürünü aynı ölçüde etkilememektedir. İşte bu noktadan hareketle, bu çalışmada, Japon çalışma kültürü, Japon çalışma kültürünün temel felsefi belirleyicilerinden biri olduğunu düşündüğümüz ve Nakane tarafından Düşey Toplum Kuramı olarak adlandırılan Japon aile yapısı ekseninde ele alınmaya çalışıl...

Development and preclinical evaluation of safety and immunogenicity of an oral ETEC vaccine containing inactivated E. coli bacteria overexpressing colonization factors CFA/I, CS3, CS5 and CS6 combined with a hybrid LT/CT B subunit antigen, administered alone and together with dmLT adjuvant.

Science.gov (United States)

Holmgren, J; Bourgeois, L; Carlin, N; Clements, J; Gustafsson, B; Lundgren, A; Nygren, E; Tobias, J; Walker, R; Svennerholm, A-M

2013-05-07

A first-generation oral inactivated whole-cell enterotoxigenic Escherichia coli (ETEC) vaccine, comprising formalin-killed ETEC bacteria expressing different colonization factor (CF) antigens combined with cholera toxin B subunit (CTB), when tested in phase III studies did not significantly reduce overall (generally mild) ETEC diarrhea in travelers or children although it reduced more severe ETEC diarrhea in travelers by almost 80%. We have now developed a novel more immunogenic ETEC vaccine based on recombinant non-toxigenic E. coli strains engineered to express increased amounts of CF antigens, including CS6 as well as an ETEC-based B subunit protein (LCTBA), and the optional combination with a nontoxic double-mutant heat-labile toxin (LT) molecule (dmLT) as an adjuvant. Two test vaccines were prepared under GMP: (1) A prototype E. coli CFA/I-only formalin-killed whole-cell+LCTBA vaccine, and (2) A "complete" inactivated multivalent ETEC-CF (CFA/I, CS3, CS5 and CS6 antigens) whole-cell+LCTBA vaccine. These vaccines, when given intragastrically alone or together with dmLT in mice, were well tolerated and induced strong intestinal-mucosal IgA antibody responses as well as serum IgG and IgA responses to each of the vaccine CF antigens as well as to LT B subunit (LTB). Both mucosal and serum responses were further enhanced (adjuvanted) when the vaccines were co-administered with dmLT. We conclude that the new multivalent oral ETEC vaccine, both alone and especially in combination with the dmLT adjuvant, shows great promise for further testing in humans. Copyright © 2013 Elsevier Ltd. All rights reserved.

Alkaline pH Is a signal for optimal production and secretion of the heat labile toxin, LT in enterotoxigenic Escherichia coli (ETEC.

Directory of Open Access Journals (Sweden)

Lucia Gonzales

Full Text Available Enterotoxigenic Escherichia coli (ETEC cause secretory diarrhea in children and travelers to endemic areas. ETEC spreads through the fecal-oral route. After ingestion, ETEC passes through the stomach and duodenum before it colonizes the lower part of the small intestine, exposing bacteria to a wide range of pH and environmental conditions. This study aimed to determine the impact of external pH and activity of the Cyclic AMP receptor protein (CRP on the regulation of production and secretion of heat labile (LT enterotoxin. ETEC strain E2863wt and its isogenic mutant E2863ΔCRP were grown in LBK media buffered to pH 5, 7 and 9. GM1 ELISA, cDNA and cAMP analyses were carried out on bacterial pellet and supernatant samples derived from 3 and 5 hours growth and from overnight cultures. We confirm that CRP is a repressor of LT transcription and production as has been shown before but we show for the first time that CRP is a positive regulator of LT secretion both in vitro and in vivo. LT secretion increased at neutral to alkaline pH compared to acidic pH 5 where secretion was completely inhibited. At pH 9 secretion of LT was optimal resulting in 600 percent increase of secreted LT compared to unbuffered LBK media. This effect was not due to membrane leakage since the bacteria were viable at pH 9. The results indicate that the transition to the alkaline duodenum and/or exposure to high pH close to the epithelium as well as activation of the global transcription factor CRP are signals that induce secretion of the LT toxin in ETEC.

EuroCirCol kick-off event

CERN Multimedia

Hardre, Julie

2015-01-01

The EuroCirCol (http://cern.ch/eurocircol) kick-off event at CERN on June 2-4 brought together 62 participants to constitute governance bodies, commit to the project plan and align the organisation, structures and processes of 16 institutions from 10 countries. The goal of the project is to conceive a post-LHC research infrastructure around a 100 km circular energy-frontier hadron collider capable of reaching 100 TeV collisions. The project officially started on June 1 and will run for four years. The total estimated budget of 11.2 million Euros includes a 2.99 million Euro contribution from the Horizon 2020 programme on developing new world-class research infrastructures (http://cordis.europa.eu/project/rcn/194962_en.html).

Sewage sludge amendment and inoculation with plant-parasitic nematodes do not facilitate the internalization of Salmonella Typhimurium LT2 in lettuce plants.

Science.gov (United States)

Fornefeld, Eva; Baklawa, Mohamed; Hallmann, Johannes; Schikora, Adam; Smalla, Kornelia

2018-05-01

Contamination of fruits and vegetables with Salmonella is a serious threat to human health. In order to prevent possible contaminations of fresh produce it is necessary to identify the contributing ecological factors. In this study we investigated whether the addition of sewage sludge or the presence of plant-parasitic nematodes foster the internalization of Salmonella enterica serovar Typhimurium LT2 into lettuce plants, posing a potential threat for human health. Greenhouse experiments were conducted to investigate whether the amendment of sewage sludge to soil or the presence of plant-parasitic nematodes Meloidogyne hapla or Pratylenchus crenatus promote the internalization of S. Typhimurium LT2 from soil into the edible part of lettuce plants. Unexpectedly, numbers of cultivable S. Typhimurium LT2 decreased faster in soil with sewage sludge than in control soil but not in root samples. Denaturing gradient gel electrophoresis analysis revealed shifts of the soil bacterial communities in response to sewage sludge amendment and time. Infection and proliferation of nematodes inside plant roots were observed but did not influence the number of cultivable S. Typhimurium LT2 in the root samples or in soil. S. Typhimurium LT2 was not detected in the leaf samples 21 and 49 days after inoculation. The results indicate that addition of sewage sludge, M. hapla or P. crenatus to soil inoculated with S. Typhimurium LT2 did not result in an improved survival in soil or internalization of lettuce plants. Copyright © 2017. Published by Elsevier Ltd.

MaLT - Combined Motor and Language Therapy Tool for Brain Injury Patients Using Kinect.

Science.gov (United States)

Wairagkar, Maitreyee; McCrindle, Rachel; Robson, Holly; Meteyard, Lotte; Sperrin, Malcom; Smith, Andy; Pugh, Moyra

2017-03-23

The functional connectivity and structural proximity of elements of the language and motor systems result in frequent co-morbidity post brain injury. Although rehabilitation services are becoming increasingly multidisciplinary and "integrated", treatment for language and motor functions often occurs in isolation. Thus, behavioural therapies which promote neural reorganisation do not reflect the high intersystem connectivity of the neurologically intact brain. As such, there is a pressing need for rehabilitation tools which better reflect and target the impaired cognitive networks. The objective of this research is to develop a combined high dosage therapy tool for language and motor rehabilitation. The rehabilitation therapy tool developed, MaLT (Motor and Language Therapy), comprises a suite of computer games targeting both language and motor therapy that use the Kinect sensor as an interaction device. The games developed are intended for use in the home environment over prolonged periods of time. In order to track patients' engagement with the games and their rehabilitation progress, the game records patient performance data for the therapist to interrogate. MaLT incorporates Kinect-based games, a database of objects and language parameters, and a reporting tool for therapists. Games have been developed that target four major language therapy tasks involving single word comprehension, initial phoneme identification, rhyme identification and a naming task. These tasks have 8 levels each increasing in difficulty. A database of 750 objects is used to programmatically generate appropriate questions for the game, providing both targeted therapy and unique gameplay every time. The design of the games has been informed by therapists and by discussions with a Public Patient Involvement (PPI) group. Pilot MaLT trials have been conducted with three stroke survivors for the duration of 6 to 8 weeks. Patients' performance is monitored through MaLT's reporting facility

ROCK inhibition stimulates SOX9/Smad3-dependent COL2A1 expression in inner meniscus cells.

Science.gov (United States)

Furumatsu, Takayuki; Maehara, Ami; Ozaki, Toshifumi

2016-07-01

Proper functioning of the meniscus depends on the composition and organization of its fibrocartilaginous extracellular matrix. We previously demonstrated that the avascular inner meniscus has a more chondrocytic phenotype compared with the outer meniscus. Inhibition of the Rho family GTPase ROCK, the major regulator of the actin cytoskeleton, stimulates the chondrogenic transcription factor Sry-type HMG box (SOX) 9-dependent α1(II) collagen (COL2A1) expression in inner meniscus cells. However, the crosstalk between ROCK inhibition, SOX9, and other transcription modulators on COL2A1 upregulation remains unclear in meniscus cells. The aim of this study was to investigate the role of SOX9-related transcriptional complex on COL2A1 expression under the inhibition of ROCK in human meniscus cells. Human inner and outer meniscus cells were prepared from macroscopically intact lateral menisci. Cells were cultured in the presence or absence of ROCK inhibitor (ROCKi, Y27632). Gene expression, collagen synthesis, and nuclear translocation of SOX9 and Smad2/3 were analyzed. Treatment of ROCKi increased the ratio of type I/II collagen double positive cells derived from the inner meniscus. In real-time PCR analyses, expression of SOX9 and COL2A1 genes was stimulated by ROCKi treatment in inner meniscus cells. ROCKi treatment also induced nuclear translocation of SOX9 and phosphorylated Smad2/3 in immunohistological analyses. Complex formation between SOX9 and Smad3 was increased by ROCKi treatment in inner meniscus cells. Chromatin immunoprecipitation analyses revealed that association between SOX9/Smad3 transcriptional complex with the COL2A1 enhancer region was increased by ROCKi treatment. This study demonstrated that ROCK inhibition stimulated SOX9/Smad3-dependent COL2A1 expression through the immediate nuclear translocation of Smad3 in inner meniscus cells. Our results suggest that ROCK inhibition can stimulates type II collagen synthesis through the cooperative activation

Unexpected Modulation of Recall B and T Cell Responses after Immunization with Rotavirus-like Particles in the Presence of LT-R192G

Directory of Open Access Journals (Sweden)

Christelle Basset

2010-08-01

Full Text Available LT-R192G, a mutant of the thermolabile enterotoxin of E. coli, is a potent adjuvant of immunization. Immune responses are generally analyzed at the end of protocols including at least 2 administrations, but rarely after a prime. To investigate this point, we compared B and T cell responses in mice after one and two intrarectal immunizations with 2/6 rotavirus-like particles (2/6-VLP and LT-R192G. After a boost, we found, an unexpected lower B cell expansion measured by flow cytometry, despite a secondary antibody response. We then analyzed CD4+CD25+Foxp3+ regulatory T cells (Tregs and CD4+CD25+Foxp3− helper T cells after in vitro (restimulation of mesenteric lymph node cells with the antigen (2/6-VLP, the adjuvant (LT-R192G or both. 2/6-VLP did not activate CD4+CD25+Foxp3− nor Foxp3+ T cells from non-immunized and 2/6-VLP immunized mice, whereas they did activate both subsets from mice immunized with 2/6-VLP in the presence of adjuvant. LT-R192G dramatically decreased CD4+CD25+Foxp3+ T cells from non-immunized and 2/6-VLP immunized mice but not from mice immunized with 2/6-VLP and adjuvant. Moreover, in this case, LT-R192G increased Foxp3 expression on CD4+CD25+Foxp3+ cells, suggesting specific Treg activation during the recall. Finally, when both 2/6-VLP and LT-R192G were used for restimulation, LT-R192G clearly suppressed both 2/6-VLP-specific CD4+CD25+Foxp3− and Foxp3+ T cells. All together, these results suggest that LT-R192G exerts different effects on CD4+CD25+Foxp3+ T cells, depending on a first or a second contact. The unexpected immunomodulation observed during the recall should be considered in designing vaccination protocols.

Technical comparison of the commercialized Racon model 21000 Portable, Reconfigurable Line Sensor (PRLS) and original Sandia/USAF prototype

International Nuclear Information System (INIS)

Blattman, D.A.

1993-01-01

The military has been moving from a global strategic response with fixed site asset protection to regional tactical response requirements. This change necessitates high security sensor systems that can be easily relocated and rapidly placed in operation by unskilled operators. The Portable, Reconfigurable Line Sensor (PRLS) was developed by Sandia National Laboratories with United States Air Force funding. Racon, Inc. is now commercializing the PRLS through a Cooperative Research and Development Agreement (CRDA) with the United States Air Force. The commercialized design of the new PRLS bi-static radar sensor benefits from the extensive field testing of the original Sandia/USAF-developed engineering prototype systems of the 1980s. Tests conducted in hot, cold, wind, rain, and snow conditions verified exceptional intruder detection capability, resistance to spoofing attempts, and insusceptibility to mutual interference and nuisance alarms caused by birds or small animals. The use of 1990's implementation technology combined with extensive testing information has resulted in significant product performance enhancements as well as cost savings. This paper compares technical features of the original Sandia/USAF prototypes with the new commercialized Racon model 21000 Portable, Reconfigurable Line Sensor. The PRLS advances the art of outdoor security to meet the Relocatable Sensor System (RSS) challenge of the 1990s

A novel missense mutation of COL5A2 in a patient with Ehlers-Danlos syndrome.

Science.gov (United States)

Watanabe, Miki; Nakagawa, Ryuji; Naruto, Takuya; Kohmoto, Tomohiro; Suga, Ken-Ichi; Goji, Aya; Kagami, Shoji; Masuda, Kiyoshi; Imoto, Issei

2016-01-01

Ehlers-Danlos syndrome (EDS) is a group of inherited connective tissue disorders characterized by hyperextensible skin, joint hypermobility and soft tissue fragility. For molecular diagnosis, targeted exome sequencing was performed on a 9-year-old male patient who was clinically suspected to have EDS. The patient presented with progressive kyphoscoliosis, joint hypermobility and hyperextensible skin without scars. Ultimately, classical EDS was diagnosed by identifying a novel, mono-allelic mutation in COL5A2 [NM_000393.3(COL5A2_v001):c.682G>A, p.Gly228Arg].

A novel missense mutation of COL5A2 in a patient with Ehlers–Danlos syndrome

Science.gov (United States)

Watanabe, Miki; Nakagawa, Ryuji; Naruto, Takuya; Kohmoto, Tomohiro; Suga, Ken-ichi; Goji, Aya; Kagami, Shoji; Masuda, Kiyoshi; Imoto, Issei

2016-01-01

Ehlers–Danlos syndrome (EDS) is a group of inherited connective tissue disorders characterized by hyperextensible skin, joint hypermobility and soft tissue fragility. For molecular diagnosis, targeted exome sequencing was performed on a 9-year-old male patient who was clinically suspected to have EDS. The patient presented with progressive kyphoscoliosis, joint hypermobility and hyperextensible skin without scars. Ultimately, classical EDS was diagnosed by identifying a novel, mono-allelic mutation in COL5A2 [NM_000393.3(COL5A2_v001):c.682G>A, p.Gly228Arg]. PMID:27656288

ER stress and basement membrane defects combine to cause glomerular and tubular renal disease resulting from Col4a1 mutations in mice

Directory of Open Access Journals (Sweden)

Frances E. Jones

2016-02-01

Full Text Available Collagen IV is a major component of basement membranes, and mutations in COL4A1, which encodes collagen IV alpha chain 1, cause a multisystemic disease encompassing cerebrovascular, eye and kidney defects. However, COL4A1 renal disease remains poorly characterized and its pathomolecular mechanisms are unknown. We show that Col4a1 mutations in mice cause hypotension and renal disease, including proteinuria and defects in Bowman's capsule and the glomerular basement membrane, indicating a role for Col4a1 in glomerular filtration. Impaired sodium reabsorption in the loop of Henle and distal nephron despite elevated aldosterone levels indicates that tubular defects contribute to the hypotension, highlighting a novel role for the basement membrane in vascular homeostasis by modulation of the tubular response to aldosterone. Col4a1 mutations also cause diabetes insipidus, whereby the tubular defects lead to polyuria associated with medullary atrophy and a subsequent reduction in the ability to upregulate aquaporin 2 and concentrate urine. Moreover, haematuria, haemorrhage and vascular basement membrane defects confirm an important vascular component. Interestingly, although structural and compositional basement membrane defects occurred in the glomerulus and Bowman's capsule, no tubular basement membrane defects were detected. By contrast, medullary atrophy was associated with chronic ER stress, providing evidence for cell-type-dependent molecular mechanisms of Col4a1 mutations. These data show that both basement membrane defects and ER stress contribute to Col4a1 renal disease, which has important implications for the development of treatment strategies for collagenopathies.

CrossTalk: The Journal of Defense Software Engineering. Volume 23, Number 1, Jan/Feb 2010

Science.gov (United States)

2010-02-01

Maintenance Group Weber State University Arrowpoint Solutions, Inc. Robbins Gioia LLC Software Technology Support Center Weber State University OO-ALC...Les Dupaix Monika Fast Robert W. Ferguson Dr. Doretta Gordon Dr. John A. “Drew” Hamilton Jr. Gary Hebert Tony Henderson Lt. Col. Brian Hermann, Ph.D

Colômbia 2010: Guerra, Paz e Eleições

Directory of Open Access Journals (Sweden)

Luiz Antônio Gusmão

2009-02-01

Full Text Available

O enfraquecimento das Farc deve polarizar as

eleições de 2010 na Colômbia entre recrudescimento

do combate e abertura à negociação.

Independent Review of the Defense Logistics Agencys Roles and Missions

Science.gov (United States)

2014-12-01

3,000 3,500 C-5 F-15 C-130 KC-135 B-1 B-52 E-3 N IIN / N SN s BoM Items Ordered BoM Items Not Ordered Orders Not on BoM Warner- Robbins ALC Tinker... Tony Poleo Fred Pribble Michael Scott BG Steven Shapiro Brig Gen Giovanni Tuck Col Rex Adee Linda Barnett Matthew Beebe CDR Tim Benesh COL...DeVincentis Lt Gen (Ret) Kathleen Gainey RADM (Ret) Mark Heinrich CAPT (Ret) Stuart Jones Eric Peltz Marc Robbins This page is intentionally blank

Adição de colágeno em pão sem glúten elaborado com farinha de arroz

Directory of Open Access Journals (Sweden)

Carolina da Silva Graça

2017-02-01

Full Text Available Resumo Os portadores da doença celíaca, uma enteropatia, apresentam intolerância ao glúten e a restrição de alimentos que o contenham mostra-se eficaz na normalização da função intestinal e das manifestações clínicas. O objetivo do presente estudo foi avaliar o efeito da adição de colágeno em pó e em fibra nas características estruturais, tecnológicas e sensoriais de pães sem glúten, elaborados com farinha de arroz. Primeiramente, foram realizadas adições de 2%, 4%, 6% e 8% de colágeno, sob as formas em pó e em fibra, em pães elaborados com farinha de arroz, sendo estes avaliados quanto às suas características tecnológicas: volume específico, firmeza do miolo e cor da crosta e do miolo. O pão que apresentou as melhores características tecnológicas foi caracterizado quanto à sua composição química (proteínas, lipídios, cinzas, umidade e carboidratos, avaliado quanto às perdas decorrentes da cocção e submetido à análise sensorial. As massas cruas dos pães adicionados com o colágeno em fibra, assim como a massa do pão padrão (sem adição de colágeno, foram avaliadas através de reologia e microscopia eletrônica de varredura. A adição de colágeno aos pães elaborados com farinha de arroz não colaborou para a formação de uma rede semelhante à formada pelo glúten. O pão da formulação com 4% de colágeno em fibra apresentou as características tecnológicas mais próximas ao esperado, não diferindo significativamente quanto à composição química, do pão padrão. Por meio da análise sensorial, constatou-se que os pães sem glúten (padrão e com 4% de colágeno em fibra obtiveram aceitação sensorial superior a 75%. Conclui-se, com o estudo, que é possível adicionar colágeno em pães sem glúten, sendo que o pão adicionado de 4% de colágeno em fibra apresentou características mais próximas às do pão padrão testado.

Development and Application of Optimization Techniques for Composite Laminates.

Science.gov (United States)

1983-09-01

Institute of Technolgy Air University in Partial Fulfillment of the Requirements for the Degree of Master of Science by Gerald V. Flanagan, S.B. Lt. USAF...global minima [9]. An informal definition of convexity is that any two points in the space can be connected by a straight line which does not pass out of...question. A quick look at gradient information suggests that too few angles (2 for example) will make the laminate sensitive to small changes in

Variability and distribution of COL1A2 (type I collagen) polymorphisms in the central-eastern Mediterranean Basin.

Science.gov (United States)

Scorrano, Gabriele; Lelli, Roberta; Martínez-Labarga, Cristina; Scano, Giuseppina; Contini, Irene; Hafez, Hani S; Rudan, Pavao; Rickards, Olga

2016-01-01

The most abundant of the collagen protein family, type I collagen is encoded by the COL1A2 gene. The COL1A2 restriction fragment length polymorphisms (RFLPs) EcoRI, RsaI and MspI in samples from several different central-eastern Mediterranean populations were analysed and found to be potentially informative anthropogenetic markers. The objective was to define the genetic variability of COL1A2 in the central-eastern Mediterranean and to shed light on its genetic distribution in human groups over a wide geographic area. PCR-RFLP analysis of EcoRI, RsaI and MspI polymorphisms of the COL1A2 gene was performed on oral swab and blood samples from 308 individuals from the central-eastern Mediterranean Basin. The genetic similarities among these groups and other populations described in the literature were investigated through correspondence analysis. Single-marker data and haplotype frequencies seemed to suggest a genetic homogeneity within the European populations, whereas a certain degree of differentiation was noted for the Egyptians and the Turks. The genetic variability in the central-eastern Mediterranean area is probably a result of the geographical barrier of the Mediterranean Sea, which separated European and African populations over time.

Proceedings of Conference on 73 Easting: Lessons from Desert Storm Via Advanced Simulation Technology Held in Alexandria, Virginia on 27-29 August 1991

Science.gov (United States)

1992-04-01

Corporation Santa Monica, CA Col Jack Thorpe, USAF Special Assistant for Simulation Defense Advanced Research Projects Agency, DIRO Arlington, VA GEN Max...4s. Ab, it says, what about A-4s? Point and click-- manufactured by K Mart corporation , last item delivered in 1972. What does it fly? It flies Mark A...armed forces of the United States, so we can get the sinergy out of training that simply is of an estimable value in combat. Thank you very much. RADM

Karzai nõuab USA-lt võitu, ähvardades muidu rahu teha / Heiki Suurkask

Index Scriptorium Estoniae

Suurkask, Heiki, 1972-

2008-01-01

Afganistani president Hamid Karzai nõuab USA-lt ajakava selle kohta, millal NATO kavatseb Talibani üle võidu saavutada. Afganistanis visiidil viibinud USA president George W. Bush avaldas toetust Barack Obama kavale keskenduda veelgi enam Afganistanis sõdimisele

Expressão do colágeno I em carcinomas epidermóides da cavidade oral

Directory of Open Access Journals (Sweden)

Martins Gabriela Botelho

2003-01-01

Full Text Available A matriz extracelular no carcinoma epidermóide da cavidade oral sofre modificações qualitativas e quantitativas durante a sua progressão. Estas alterações parecem possibilitar os processos de invasão e metástase que caracterizam as neoplasias malignas e são mais evidentes na interface tumor-hospedeiro. No nosso estudo, 23 biópsias incisionais de carcinomas epidermóides de diversas localizações da cavidade oral foram classificadas segundo o sistema de graduação histológica preconizado por Anneroth et al.¹ (1987. Foram utilizadas as colorações pela H. E. e a do tipo picrosirius, específica para colágeno. A expressão imuno-histoquímica do colágeno tipo I foi analisada em lesões com diferentes escores histológicos de malignidade. Pôde-se concluir que o colágeno apresenta diferentes padrões morfológicos, histoquímicos e imuno-histoquímicos em lesões com altos e baixos escores de malignidade.

Linkage analysis in a family with Stickler syndrome leads to the exclusion of the COL2A1 locus

Energy Technology Data Exchange (ETDEWEB)

Mottes, M.; Zolezzi, F.; Pignatti, P.F. [Univ. of Verona (Italy)

1994-09-01

Hereditary arthro-ophtalmopathy (AO) or Stickler Syndrome (MIM No. 10830) is a dominantly inherited disorder characterized by vitro-retinal degeneration and other connective tissue disturbances. Mutations in the COL2A1 gene, coding for type II collagen chains, have been described in a few patients. The wide spectrum of clinical manifestations is presumably due to genetic heterogeneity, since only about 50% of the Stickler families so far studied show cosegregation of the disease with the COL2A1 locus. We have investigated a large pedigree (19 individuals of whom 9 are affected) in which severe myopia with vitro-retinal degeneration consegregated with joint laxity, recurrent inguinal hernias, and degenerative changes of the hip and the knee. The 3{prime} end COL2A1 VNTR polymorphism was utilized for linkage analysis. In order to get the maximum informativity, we have analyzed the allelic microheterogeneity of this VNTR, due to the repeat sequence variation, by means of a single strand polymorphism. Mendelian inheritance of the different single strands was observed as expected. Discordance of segregation between the disease and the COL2A1 locus was thus established inequivocally in this family.

The Antiaircraft Journal. Volume 97, Number 4, July-August 1954

Science.gov (United States)

1954-08-01

Col. Wood- row L. Nelson, Executive; Major Harry E. Bock, Jr., S2 and S3; Capt. Jesse N. Worsham, TI&E Officer; Lt. Bernard E. Reiter, Assistant S3; Lt...Major William J. Fling, S4, leaves for Hq. Fifth Army in July; Major Alfred H. Fierke, S2, for the 17th AAA Group; Major Bernard A. Gilman, for .USAR...USAFFE. {:onerly, U. E., to 758th FA Bn., Ft. Bragg. Cornwell , B. R., to AA&GM Qr., Ft. Bliss. Crews, D. J., to USAR Adv. Gp., Rock Islane, Illinois

A Game of Simon Says: Latin America’s Left Turn and Its Effects on US Security

Science.gov (United States)

2008-05-01

Fred P. Stone, Lt Col, PhD, Director of Research John T. Ackerman, PhD, Series Editor Kathleen Mahoney-Norris, PhD, Essay Advisor Air University...lopsided than even the lyrics from the 966 Beatles’ song “Taxman” envisioned. Alvaro Hurtado of the Interna- tional Labour Organization states that

Support of positive association in family-based genetic analysis between COL27A1 and Tourette syndrome.

Science.gov (United States)

Liu, Shiguo; Yu, Xiaoxia; Xu, Quanchen; Cui, Jiajia; Yi, Mingji; Zhang, Xinhua; Ge, Yinlin; Ma, Xu

2015-08-03

Recently, a genome-wide association study has indicated associations between single nucleotide polymorphisms in the Collagen Type XXVII Alpha 1 gene (COL27A1) and Tourette syndrome in several ethnic populations. To clarify the global relevance of the previously identified SNPs in the development of Tourette syndrome, the associations between polymorphisms in COL27A1 and Tourette syndrome were assessed in Chinese trios. PCR-directed sequencing was used to evaluate the genetic contributions of three SNPs in COL27A1(rs4979356, rs4979357 and rs7868992) using haplotype relative risk (HRR) and transmission disequilibrium tests (TDT) with a total of 260 Tourette syndrome trios. The family-based association was significant between Tourette syndrome and rs4979356 (TDT: χ2 = 4.804, P = 0.033; HRR = 1.75, P = 0.002; HHRR = 1.32, P = 0.027), and transmission disequilibrium was suspected for rs4979357 (TDT: χ2 = 3.969, P = 0.053; HRR = 1.84, P = 0.001; HHRR = 1.29, P = 0.044). No statistically significant allele transfer was found for rs7868992 (TDT: χ2 = 2.177, P = 0.158). Although the TDT results did not remain significant after applying the conservative Bonferroni correction (p = 0.005), the significant positive HRR analysis confirmed the possibility of showing transmission disequilibrium, which provides evidence for an involvement of COL27A1in the development of TS. However, these results need to be verified with larger datasets from different populations.

Study on characterization of interaction layer between U-10wt%Mo alloy and LT24Al

International Nuclear Information System (INIS)

Chen Jiangang; Yin Changgeng; Sun Changlong; Pang Xiaoxuan; Liu Yunming

2009-01-01

The characterization of interaction layer(IL) between U-10wt%Mo alloy and LT24 Al was studied in detail in this paper. Sandwich structured U-Mo/LT24 Al diffusion couples were hot pressed at different temperature and pressure for different time. Then they were analyzed by Optical Microscope (OM) and Scanning Electron Microscope (SEM) to observe the width of the IL. The distribution of the diffusion elements and the phases in the IL were determined by Energy Dispersive Spectroscopy (EDS) and X Ray Diffraction (XRD). Analysis results are as follows: the diffusion manner was reaction diffusion, and diffusion direction mainly was that Al atoms diffused to U-Mo alloy; diffusion mechanism was vacancy diffusion and growth kinetics showed reaction was controlled by the diffusion speed; the IL containing single phase was constituted mainly by (U, Mo) Al 3 ; the IL containing two phases or more was constituted mainly by (U, Mo) Al 3 and (U, Mo) Al 4 and Al 20 Mo 2 U; and Si impurity in the LT24 Al was easy to enrich in the IL which showed Si added to Al could play positive role on improve compatibility between U-Mo and Al. (authors)

AutoCAD 2012 and AutoCAD LT 2012 Bible

CERN Document Server

Finkelstein, Ellen

2011-01-01

The latest version of this perennial favorite, in-depth, reference-tutorial This top-selling book has been updated by AutoCAD guru and author Ellen Finkelstein to provide you with the very latest coverage of both AutoCAD 2012 and AutoCAD LT 2012. It begins with a Quick Start tutorial, so you start creating right away. From there, the book covers so much in-depth material on AutoCAD that it is said that even Autodesk employees keep this comprehensive book at their desks. A DVD is included that features before-and-after drawings of all the tutorials and plenty of great examples from AutoCAD prof

Molecular Evolution and Phylogenetic Analysis of Eight COL Superfamily Genes in Group I Related to Photoperiodic Regulation of Flowering Time in Wild and Domesticated Cotton (Gossypium) Species

Science.gov (United States)

Zhang, Rui; Ding, Jian; Liu, Chunxiao; Cai, Caiping; Zhou, Baoliang; Zhang, Tianzhen; Guo, Wangzhen

2015-01-01

Flowering time is an important ecological trait that determines the transition from vegetative to reproductive growth. Flowering time in cotton is controlled by short-day photoperiods, with strict photoperiod sensitivity. As the CO-FT (CONSTANS-FLOWER LOCUS T) module regulates photoperiodic flowering in several plants, we selected eight CONSTANS genes (COL) in group I to detect their expression patterns in long-day and short-day conditions. Further, we individually cloned and sequenced their homologs from 25 different cotton accessions and one outgroup. Finally, we studied their structures, phylogenetic relationship, and molecular evolution in both coding region and three characteristic domains. All the eight COLs in group I show diurnal expression. In the orthologous and homeologous loci, each gene structure in different cotton species is highly conserved, while length variation has occurred due to insertions/deletions in intron and/or exon regions. Six genes, COL2 to COL5, COL7 and COL8, exhibit higher nucleotide diversity in the D-subgenome than in the A-subgenome. The Ks values of 98.37% in all allotetraploid cotton species examined were higher in the A-D and At-Dt comparison than in the A-At and D-Dt comparisons, and the Pearson’s correlation coefficient (r) of Ks between A vs. D and At vs. Dt also showed positive, high correlations, with a correlation coefficient of at least 0.797. The nucleotide polymorphism in wild species is significantly higher compared to G. hirsutum and G. barbadense, indicating a genetic bottleneck associated with the domesticated cotton species. Three characteristic domains in eight COLs exhibit different evolutionary rates, with the CCT domain highly conserved, while the B-box and Var domain much more variable in allotetraploid species. Taken together, COL1, COL2 and COL8 endured greater selective pressures during the domestication process. The study improves our understanding of the domestication-related genes/traits during cotton

Targeted deletion of RANKL in M cell inducer cells by the Col6a1-Cre driver.

Science.gov (United States)

Nagashima, Kazuki; Sawa, Shinichiro; Nitta, Takeshi; Prados, Alejandro; Koliaraki, Vasiliki; Kollias, George; Nakashima, Tomoki; Takayanagi, Hiroshi

2017-11-04

The gut-associated lymphoid tissues (GALTs), including Peyer's patches (PPs), cryptopatches (CPs) and isolated lymphoid follicles (ILFs), establish a host-microbe symbiosis by the promotion of immune reactions against gut microbes. Microfold cell inducer (MCi) cells in GALTs are the recently identified mesenchymal cells that express the cytokine RANKL and initiate bacteria-specific immunoglobulin A (IgA) production via induction of microfold (M) cell differentiation. In the previous study, the Twist2-Cre driver was utilized for gene deletion in mesenchymal cells including MCi cells. In order to investigate MCi cells more extensively, it will be necessary to develop experimental tools in addition to the Twist2-Cre driver mice and characterize such drivers in specificity and efficiency. Here we show that M cell differentiation and IgA production are impaired in the targeted deletion of RANKL by the Col6a1-Cre driver. We compared Col6a1-Cre with Twist2-Cre in terms of the specificity for mesenchymal cells in GALTs. Col6a1-Cre CAG-CAT-EGFP mice exhibited EGFP expression in podoplanin + CD31 - cells including MCi cells, while Twist2-Cre mice were shown to target endothelial cells and podoplanin + CD31 - cells. Tnfsf11 fl/Δ Col6a1-Cre mice exhibited the absence of M cells and severe IgA reduction together with an alteration in gut microbial composition. Moreover, we analyzed germ free mice to test whether changes in the microbiota are the cause of M cell deficiency. M cell differentiation was normal in the CPs/ILFs of germ free mice, indicating that MCi cells induce M cells independently of microbial colonization. This study demonstrates that Col6a1-Cre driver mice are as useful as Twist2-Cre driver mice for functional analyses of GALT-resident mesenchymal cells, including MCi cells. Copyright © 2017 Elsevier Inc. All rights reserved.

Clonal relatedness of enterotoxigenic Escherichia coli (ETEC) strains expressing LT and CS17 isolated from children with diarrhoea in La Paz, Bolivia.

Science.gov (United States)

Rodas, Claudia; Klena, John D; Nicklasson, Matilda; Iniguez, Volga; Sjöling, Asa

2011-01-01

Enterotoxigenic Escherichia coli (ETEC) is a major cause of traveller's and infantile diarrhoea in the developing world. ETEC produces two toxins, a heat-stable toxin (known as ST) and a heat-labile toxin (LT) and colonization factors that help the bacteria to attach to epithelial cells. In this study, we characterized a subset of ETEC clinical isolates recovered from Bolivian children under 5 years of age using a combination of multilocus sequence typing (MLST) analysis, virulence typing, serotyping and antimicrobial resistance test patterns in order to determine the genetic background of ETEC strains circulating in Bolivia. We found that strains expressing the heat-labile (LT) enterotoxin and colonization factor CS17 were common and belonged to several MLST sequence types but mainly to sequence type-423 and sequence type-443 (Achtman scheme). To further study the LT/CS17 strains we analysed the nucleotide sequence of the CS17 operon and compared the structure to LT/CS17 ETEC isolates from Bangladesh. Sequence analysis confirmed that all sequence type-423 strains from Bolivia had a single nucleotide polymorphism; SNP(bol) in the CS17 operon that was also found in some other MLST sequence types from Bolivia but not in strains recovered from Bangladeshi children. The dominant ETEC clone in Bolivia (sequence type-423/SNP(bol)) was found to persist over multiple years and was associated with severe diarrhoea but these strains were variable with respect to antimicrobial resistance patterns. The results showed that although the LT/CS17 phenotype is common among ETEC strains in Bolivia, multiple clones, as determined by unique MLST sequence types, populate this phenotype. Our data also appear to suggest that acquisition and loss of antimicrobial resistance in LT-expressing CS17 ETEC clones is more dynamic than acquisition or loss of virulence factors.

Clonal Relatedness of Enterotoxigenic Escherichia coli (ETEC) Strains Expressing LT and CS17 Isolated from Children with Diarrhoea in La Paz, Bolivia

Science.gov (United States)

Rodas, Claudia; Klena, John D.; Nicklasson, Matilda; Iniguez, Volga; Sjöling, Åsa

2011-01-01

Background Enterotoxigenic Escherichia coli (ETEC) is a major cause of traveller's and infantile diarrhoea in the developing world. ETEC produces two toxins, a heat-stable toxin (known as ST) and a heat-labile toxin (LT) and colonization factors that help the bacteria to attach to epithelial cells. Methodology/Principal Findings In this study, we characterized a subset of ETEC clinical isolates recovered from Bolivian children under 5 years of age using a combination of multilocus sequence typing (MLST) analysis, virulence typing, serotyping and antimicrobial resistance test patterns in order to determine the genetic background of ETEC strains circulating in Bolivia. We found that strains expressing the heat-labile (LT) enterotoxin and colonization factor CS17 were common and belonged to several MLST sequence types but mainly to sequence type-423 and sequence type-443 (Achtman scheme). To further study the LT/CS17 strains we analysed the nucleotide sequence of the CS17 operon and compared the structure to LT/CS17 ETEC isolates from Bangladesh. Sequence analysis confirmed that all sequence type-423 strains from Bolivia had a single nucleotide polymorphism; SNPbol in the CS17 operon that was also found in some other MLST sequence types from Bolivia but not in strains recovered from Bangladeshi children. The dominant ETEC clone in Bolivia (sequence type-423/SNPbol) was found to persist over multiple years and was associated with severe diarrhoea but these strains were variable with respect to antimicrobial resistance patterns. Conclusion/Significance The results showed that although the LT/CS17 phenotype is common among ETEC strains in Bolivia, multiple clones, as determined by unique MLST sequence types, populate this phenotype. Our data also appear to suggest that acquisition and loss of antimicrobial resistance in LT-expressing CS17 ETEC clones is more dynamic than acquisition or loss of virulence factors. PMID:22140423

Columbian Secrets: Cristóbal Colón by Leonardo Balada and Antonio Gala

Directory of Open Access Journals (Sweden)

José Elías Gutiérrez Meza

2016-11-01

Full Text Available The play Cristóbal Colón premiered in 1989 at the Teatro del Liceu. Composed by the musician Leonardo Balada and the playwright Antonio Gala, this opera was commissioned by the Sociedad Estatal Quinto Centenario as part of the festivities organized by the Spanish government to commemorate the Discovery of America. Unlike the so-called "des-celebración del 92", Cristóbal Colón champions the figure of the discoverer and emphasizes the participation of Spain through the praised role of Martín Alonso Pinzón. However, the opera was cut at the last minute. This article asserts that such alteration may have been the result of political censorship attempting to counteract the criticism of the Discovery of America contained in the original version.

Produção de identidades nos trabalhos apresentados em colóquios luso-brasileiros de 2002 e 2004

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Leonardo Dias Ferreira

2017-12-01

Full Text Available O presente artigo trata-se do estudo da internacionalização do campo do currículo com enfoque em trabalhos apresentados em colóquios luso-brasileiros de 2002 e 2004 sobre questões curriculares e produção de identidades. Para isso, faremos uma contextualização histórica do colóquio luso-brasileiro, depois refletiremos sobre a internacionalização do campo do currículo e a visão desta concepção presente nos editoriais dos colóquios Luso-Brasileiros e na última seção falaremos sobre a categoria identidade. Concluiu-se que  entender a relação direta de questões sobre multiculturalidade e formação da identidade do sujeito envolvido no processo de ensino-aprendizagem escolar é essencial para que a escola seja voltada para formação e interação social, de tal modo, que os alunos possam compartilhar suas crenças e valores e aprender a respeitar, reconhecer e valorizar as diferenças do outro. Palavras Chaves: internacionalização do campo do currículo; colóquios luso-brasileiros, identidade.

A Game Theoretic Model for the Optimal Disposition of Integrated Air Defense System Assets

Science.gov (United States)

2015-03-26

fellows of Miami Valley Philosophical and Lifting Society v Acknowledgements I want to thank LTC Brian J. Lunday and Lt Col Matthew J. Robbins for...on the defender’s valuation of tar- gets. However, if we assume that Hamas, the attacker, knows the layout of Israel’s defense, they may attack

Are Marines a Better Fit for Afghanistan

Science.gov (United States)

2008-03-01

FOR AFGHANISTAN? by Lieutenant Colonel William M. Tart Unites States Air Force Colonel John A. Terrell Project Adviser This SRP is submitted in...December 2007. 7 Telephone interview with Lt Col James Kendall, USMC, USMC PP&O, 11 Jan 08. 8 Anne Flaherty, “Marine Commandant Says He Is Concerned Iraq

75 FR 77044 - Blocking of Specially Designated National Pursuant to Executive Order 13413

Science.gov (United States)

2010-12-10

..., the International Emergency Economic Powers Act (50 U.S.C. 1701 et seq.) (IEEPA), section 5 of the... seven individuals in the Annex to the Order as subject to these economic sanctions. Section 1 of the..., Rwanda; nationality Rwanda; Lt. Col. (individual) [DRCONGO] 2. ZIMURINDA, Innocent; DOB 1 Sep 1972; alt...

An Analysis of the Effectiveness of Air Force Information Management Officer Training Based on the Perceived Needs of Current Information Management Officers

Science.gov (United States)

1992-12-01

proper perspective. Finally, I wish to thank my thesis advisors , Lt Col John Huguley Jr. and Dr Ben Williams. Together they made a most supportive and...Microcomputer System Implementation in Small Businesses," Journal of Systems Management 41:7-9 (June 1990). 35. Schrage, Michael. "Meet the Robo -Boss

Projecting Politics: The Grapes of Wrath Les Raisins de la colère : Une critique politique

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Stephen J. Whitfield

2008-01-01

Full Text Available Publié en 1939 et adapté à l’écran l’année suivante, Les Raisins de la colère, roman de John Steinbeck, eut un impact immédiat et extraordinaire pour le portrait sans concession qu’il dressait d’un capitalisme américain qui semblait avoir abandonné sa progéniture la plus loyale, la plus assidue et la plus courageuse, celle qui labourait la terre en plein cœur de la république. Écrit à la toute fin de la « Décennie rouge », mais juste avant que les États-Unis entrent dans la Seconde Guerre mondiale, Les Raisins de la colère exprimaient, d’une certaine manière, la colère accumulée contre un système économique qui avait trahi le prolétariat rural. Le roman tout comme le film de John Ford (1940 furent compris par le grand public de l’époque, et bien des années plus tard, comme une protestation clairement gauchiste, voire radicale, contre une telle injustice. Mais cet essai montre combien les idées politiques de ces œuvres jumelées sont en réalité difficiles à saisir et combien il est hasardeux de définir, quel que soit l’effort rétrospectif, la critique que Steinbeck et Ford ont proposée dans leur version des Raisins de la colère, une œuvre inoubliable, déchirante mais ambiguë sur le plan politique.

The impact of ColRS two-component system and TtgABC efflux pump on phenol tolerance of Pseudomonas putida becomes evident only in growing bacteria

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Kivisaar Maia

2010-04-01

Full Text Available Abstract Background We have recently found that Pseudomonas putida deficient in ColRS two-component system is sensitive to phenol and displays a serious defect on solid glucose medium where subpopulation of bacteria lyses. The latter phenotype is significantly enhanced by the presence of phenol in growth medium. Here, we focused on identification of factors affecting phenol tolerance of the colR-deficient P. putida. Results By using transposon mutagenesis approach we identified a set of phenol-tolerant derivatives of colR-deficient strain. Surprisingly, half of independent phenol tolerant clones possessed miniTn5 insertion in the ttgABC operon. However, though inactivation of TtgABC efflux pump significantly enhanced phenol tolerance, it did not affect phenol-enhanced autolysis of the colR mutant on glucose medium indicating that phenol- and glucose-caused stresses experienced by the colR-deficient P. putida are not coupled. Inactivation of TtgABC pump significantly increased the phenol tolerance of the wild-type P. putida as well. Comparison of phenol tolerance of growing versus starving bacteria revealed that both ColRS and TtgABC systems affect phenol tolerance only under growth conditions and not under starvation. Flow cytometry analysis showed that phenol strongly inhibited cell division and to some extent also caused cell membrane permeabilization to propidium iodide. Single cell analysis of populations of the ttgC- and colRttgC-deficient strains revealed that their membrane permeabilization by phenol resembles that of the wild-type and the colR mutant, respectively. However, cell division of P. putida with inactivated TtgABC pump seemed to be less sensitive to phenol than that of the parental strain. At the same time, cell division appeared to be more inhibited in the colR-mutant strain than in the wild-type P. putida. Conclusions ColRS signal system and TtgABC efflux pump are involved in the phenol tolerance of P. putida. However, as

A experiência com arte na Colônia Juliano Moreira na década de 1950

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João Henrique Queiroz de Araújo

Full Text Available Resumo Os trabalhos de Osório César e de Nise da Silveira costumam se destacar quando se investiga a relação entre expressão artística e saberes psi no Brasil em torno da década de 1950. Porém, outras experiências ocorreram no mesmo período, como as atividades de pintura realizadas na Seção de Praxiterapia da Colônia Juliano Moreira, em Jacarepaguá, no município do Rio de Janeiro. A ausência de pesquisas sobre essa experiência suscitou uma busca por documentos que culminou na análise dos números do Boletim da Colônia Juliano Moreira e demais fontes. Os resultados apontam características peculiares da experiência artística na colônia, como o incentivo à cópia e reprodução de fotografias como método terapêutico, o que a diferencia das outras experiências mencionadas.

Population haplotype analysis and evolutionary relations of the COL2A1 gene

NARCIS (Netherlands)

Meulenbelt, I.; Williams, G.J.; Koppele, J.M.T.E.; Giessen, G.C.D.E. van; Slagboom, P.E.

1996-01-01

We have determined the allele frequencies mad pairwise linkage disequilibria of restriction fragment length polymorphisms (RFLPs) distributed over the entire COL2A1 gene (spanning 23 · 6 kb) in a population of unrelated Dutch Caucasians. Pairwise linkage disequilibrium analysis of RFLP sites between

ENVIRONMENTAL TECHNOLOGY VERIFICATION REPORT LASER TOUCH AND TECHNOLOGIES, LLC LASER TOUCH MODEL LT-B512

Science.gov (United States)

The Environmental Technology Verification report discusses the technology and performance of Laser Touch model LT-B512 targeting device manufactured by Laser Touch and Technologies, LLC, for manual spray painting operations. The relative transfer efficiency (TE) improved an avera...

Las cuentas del cuarto viaje de Cristóbal Colón

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Gil, Juan

2003-12-01

Full Text Available The payroll of the fourth voyage of Christopher Columbus is here published for the fisrt time, signed by Admiral himself. A commentary is also furnished.

Se publican por primera vez, con comentario, las cuentas del cuarto viaje de Cristóbal Colón, firmadas por el propio almirante.

Avaliação da preferência alimentar em modelo de poliartrite induzida por colágeno

OpenAIRE

Eduarda Correa Freitas

2014-01-01

Introdução: Artrite reumatoide é uma doença inflamatória crônica associada a alterações no metabolismo proteico e energético. Objetivos: Investigar a preferência alimentar durante o desenvolvimento da inflamação crônica em ratas com artrite induzida por colágeno. Métodos: Ratas Wistar fêmeas foram alocadas em dois grupos: controle (CO, n=10) e artrite induzida por colágeno (CIA; n=11) e expostas simultaneamente a quatro diferentes dietas (padrão, hipercalórica, hiperlipidica e hiperproteica)....

The Tongue and Quill

Science.gov (United States)

1997-06-30

gobbledygook. (Mary Maverick, a congressperson from Texas, coined this term “gobbledygook” for wordy, pompous, overweight prose that confuses and irritates...Colonel Fernando Ordoñez, of the Peruvian air force in Lima, Peru , will be here tomorrow. Lt Col Ordoñez of Lima, Peru , will be here tomorrow. [The comma

Variables físicas, químicas y biológicas del suelo sobre las poblaciones de colémbolos en Costa Rica

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Cesar Guillen

2006-01-01

Full Text Available Se ha discutido que las variables físicas, químicas y biológicas del suelo influyen sobre las poblaciones de colémbolos; sin embargo, la información acerca de la interacción de estas variables, en conjunto, con relación a la diversidad y abundancia de colémbolos, es escasa. En el presente estudio se determinó la relación entre estas variables y la diversidad y abundancia de colémbolos en los suelos de un bosque primario, un bosque secundario y un cafetal en el Parque Nacional Tapantí. El bosque primario presentó los contenidos más altos de materia orgánica y biomasa microbiana, contrario a lo sucedido con la resistencia a la penetración y el pH. Los resultados mostraron una clara asociación entre estas variables y algunas especies de colémbolos, lo cual indica que los cambios en la estructura de las comunidades de colémbolos, por efecto de los cambios en las variables físicas, químicas y biológicas del suelo, pueden ser utilizados como un bioindicador de calidad del suelo y de manejo de los ecosistemas.

Collagen Type I alpha1 (COL1A1 Gene Polymorphism and Bone Mineral Density in Postmenopausal Kazakh Women

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Akbota Aitkulova

2014-12-01

Full Text Available Introduction: Single nucleotide polymorphism (SNP at the collagen type I alpha 1 gene (COL1A1 rs1800012 has been widely studied and has shown an association with bone mineral density (BMD and fractures. A minor allele TT of this SNP was found to be greatly overrepresented in individuals with fractures compared to controls, thus becoming a good predictor of  increased fracture risk. The aim of this investigation was to evaluate potential association between COL1A1 gene polymorphism and osteoporosis in Kazakh postmenopausal women.Methods: The study population included 103 postmenopausal women recruited from Pavlodar and Almaty clinics. BMD was measured using DEXA. Genomic DNA was extracted from peripheral venous blood of study participants with Wizard® Genomic DNA Purification Kit (Promega, USA. Detection of COL1A1 +1245G/T (Sp1 polymorphism was done by the TaqMan® SNP Genotyping Assay of real-time PCR.Results: Densitometry results revealed 36 osteoporotic, 42 osteopenic, and 25 normal postmenopausal women. Data analysis of 1245G>T polymorphism in COL1A1 gene in the group of women with osteopenia and osteoporosis revealed deviation from Hardy-Weinberg equilibrium. The mutant TT genotype was prevalent compared to the heterozygous genotype GT in both groups. Distributions were 83% GG, 3% GT, and 14% TT in the group with osteopenia and 80% GG, 6% GT, and 14% TT in the group with osteoporosis. The distribution of genotypes frequency in the group of normal postmenopausal women was 76% GG, 16% GT, and 8% TT.Conclusion: These results suggest that TT genotype of COL1A1 +1245G/T (Sp1 polymorphism is associated with risk of postmenopausal osteoporosis in Kazakh women. Further studies involving a larger number of women are needed to clarify the relationship of this polymorphism with risk of osteoporosis. 

El almirante Cristóbal Colón y la ciudad de Valladolid

OpenAIRE

Varela Marcos , Jesús

2006-01-01

Pretendemos aunar todas las presencias de Colón en la ciudad de Valladolid en el año de la conmemoración de su muerte. Para ello hemos rastreado en la documentación todos los momentos en que el genovés estuvo en dicha villa.

Marker-Assisted Selection of Xa21 Conferring Resistance to Bacterial Leaf Blight in indica Rice Cultivar LT2

Institute of Scientific and Technical Information of China (English)

Hue Thi NGUYEN; Trung Nguyen DINH; Nakano TOSHITSUGU; Liet Van VU; Quang Hong VU; Tan Van MAI; Thu Thi NGUYEN; Lam Duc VU; Tung Thanh NGUYEN; Long Viet NGUYEN; Hien Thu Thi VU; Hue Thi NONG

2018-01-01

Bacterial leaf blight of rice (BLB), caused by Xanthomonas oryzae pv. oryzae, is one of the most destructive diseases in Asian rice fields. A high-quality rice variety, LT2, was used as the recipient parent. IRBB21, which carries the Xa21 gene, was used as the donor parent. The resistance gene Xa21 was introduced into LT2 by marker-assisted backcrossing. Three Xoo races were used to inoculate the improved lines following the clipping method. Eleven BC3F3lines carrying Xa21 were obtained based on molecular markers and agronomic performance. The 11 lines were then inoculated with the three Xoo races. All the 11 improved lines showed better resistance to BLB than the recipient parent LT2. Based on the level of resistance to BLB and their agronomic performance, five lines (BC3F35.1.5.1, BC3F35.1.5.12, BC3F38.5.6.44, BC3F3 9.5.4.1 and BC3F39.5.4.23) were selected as the most promising for commercial release. These improved lines could contribute to rice production in terms of food security.

Different NaCl-Induced Calcium Signatures in the Arabidopsis thaliana Ecotypes Col-0 and C24

KAUST Repository

Schmöckel, Sandra M.

2015-02-27

A common feature of stress signalling pathways are alterations in the concentration of cytosolic free calcium ([Ca2+]cyt), which allow the specific and rapid transmission of stress signals through a plant after exposure to a stress, such as salinity. Here, we used an aequorin based bioluminescence assay to compare the NaCl-induced changes in [Ca2+]cyt of the Arabidopsis ecotypes Col-0 and C24. We show that C24 lacks the NaCl specific component of the [Ca2+]cyt signature compared to Col-0. This phenotypic variation could be exploited as a screening methodology for the identification of yet unknown components in the early stages of the salt signalling pathway.

Different NaCl-Induced Calcium Signatures in the Arabidopsis thaliana Ecotypes Col-0 and C24

KAUST Repository

Schmö ckel, Sandra M.; Garcia, Alexandre F.; Berger, Bettina; Tester, Mark A.; Webb, Alex A. R.; Roy, Stuart J.

2015-01-01

A common feature of stress signalling pathways are alterations in the concentration of cytosolic free calcium ([Ca2+]cyt), which allow the specific and rapid transmission of stress signals through a plant after exposure to a stress, such as salinity. Here, we used an aequorin based bioluminescence assay to compare the NaCl-induced changes in [Ca2+]cyt of the Arabidopsis ecotypes Col-0 and C24. We show that C24 lacks the NaCl specific component of the [Ca2+]cyt signature compared to Col-0. This phenotypic variation could be exploited as a screening methodology for the identification of yet unknown components in the early stages of the salt signalling pathway.

Toxicidade córneo-conjuntival do colírio de iodo-povidona: estudo experimental

Directory of Open Access Journals (Sweden)

Santos Namir Clementino

2003-01-01

Full Text Available OBJETIVO: Avaliar a toxicidade ocular do colírio de iodo-povidona a 2,5% e a 0,5% sobre a superfície ocular, na regeneração do epitélio corneal e as alterações histopatológicas da córnea. MÉTODOS: Realizaram-se estudos experimentais consecutivos em coelhos albinos, nos quais se fez a ablação do epitélio de uma área circular central da córnea de 6,5 mm de diâmetro. Em cada experimento foram utilizados 20 animais (40 olhos, sendo que no olho direito foi instilado o colírio de iodo-povidona (caso e no olho esquerdo água destilada (controle, em intervalos de uma hora, durante três dias consecutivos. Durante o experimento, os animais foram submetidos a exames biomicroscópicos diários para avaliação da superfície córneo-conjuntival e realização de fotografias seriadas da área sem epitélio, corada com fluoresceína, para medida da área projetada da lesão com auxílio de analisador de imagem computadorizado. No final do experimento, os animais foram sacrificados para avaliação histopatológica das córneas. RESULTADOS: O colírio de iodo-povidona a 2,5% comprometeu a regeneração epitelial, causou conjuntivite em 100% dos olhos, com produção de secreção de aspecto mucoso em 80%, ceratite ponteada em 40% e edema estrômico leve em 10% dos casos. Os achados histopatológicos foram úlcera de córnea, degeneração hidrópica das células endoteliais e infiltrado inflamatório com predomínio de eosinófilos em 100% dos casos. Nos olhos em que se instilou iodo-povidona a 0,5%, assim como nos controles, observou-se completa regeneração da lesão epitelial (p<0,001 após 72 horas do início do experimento. Do ponto de vista histopatológico, epitelização normal em todos os casos e controles, em apenas um caso observou-se discreto infiltrado de leucócitos perilímbicos. CONCLUSÃO: A toxicidade ocular do colírio de iodo-povidona é dependente da concentração da solução, sendo que o colírio a 2,5% mostrou

Airpower Leadership on the Front Line: Lt Gen George H. Brett and Combat Command

Science.gov (United States)

2006-09-01

was assigned the additional duty of unit photographer and historian, bequeathing a photo album depicting his adventures to his oldest daughter.10 Lt...his fellow residents with piano music when the men were off duty.21 At the close of the war Brett was a temporary major and listed among officers who

Extensive Analysis of GmFTL and GmCOL Expression in Northern Soybean Cultivars in Field Conditions.

Science.gov (United States)

Guo, Guangyu; Xu, Kun; Zhang, Xiaomei; Zhu, Jinlong; Lu, Mingyang; Chen, Fulu; Liu, Linpo; Xi, Zhang-Ying; Bachmair, Andreas; Chen, Qingshan; Fu, Yong-Fu

2015-01-01

The FLOWERING LOCUS T (FT) gene is a highly conserved florigen gene among flowering plants. Soybean genome encodes six homologs of FT, which display flowering activity in Arabidopsis thaliana. However, their contributions to flowering time in different soybean cultivars, especially in field conditions, are unclear. We employed six soybean cultivars with different maturities to extensively investigate expression patterns of GmFTLs (Glycine max FT-like) and GmCOLs (Glycine max CO-like) in the field conditions. The results show that GmFTL3 is an FT homolog with the highest transcript abundance in soybean, but other GmFTLs may also contribute to flower induction with different extents, because they have more or less similar expression patterns in developmental-, leaf-, and circadian-specific modes. And four GmCOL genes (GmCOL1/2/5/13) may confer to the expression of GmFTL genes. Artificial manipulation of GmFTL expression by transgenic strategy (overexpression and RNAi) results in a distinct change in soybean flowering time, indicating that GmFTLs not only impact on the control of flowering time, but have potential applications in the manipulation of photoperiodic adaptation in soybean. Additionally, transgenic plants show that GmFTLs play a role in formation of the first flowers and in vegetative growth.

The Variants Within the COL5A1 Gene are Associated with Reduced Risk of Anterior Cruciate Ligament Injury in Skiers

Directory of Open Access Journals (Sweden)

Stępień-Słodkowska Marta

2015-03-01

Full Text Available The purpose of this study was to examine the association of the BstUI RFLP C/T (rs 12722 and DpnII RFLP C/T (rs 13946 COL5A1 polymorphisms, individually and as haplotypes, with anterior cruciate ligament ruptures in recreational skiers. Subjects were 138 male recreational skiers with surgically diagnosed primary anterior cruciate ligament ruptures. The control group consisted of 183 apparently healthy male recreational skiers, who were without any self-reported history of ligament or tendon injury. DNA was extracted from buccal cells donated by the subjects and genotyping was carried out using real-time PCR. The genotype distributions for both polymorphisms met Hardy- Weinberg expectations in both groups. There were no significant differences in genotype distribution of allele frequencies of COL5A1 BstUI RFLP C/T and COL5A1 DpnII RFLP C/T polymorphisms between the ACL rupture and control groups. The T-T (BstUI RFLP T, DpnII RFLP T haplotype was the most common (55.6%. The haplotype T-C was not present in any of the subjects. There was an underrepresentation tendency of the C-T haplotype in the study group compared to controls under recessive mode of inheritance. Higher frequency of the COL5A1 BstUI RFLP C/T and COL5A1DpnII RFLP C/T polymorphisms haplotype is associated with reduced risk of anterior cruciate ligament injury in a group of apparently healthy male recreational skiers.

Projecting Politics: The Grapes of Wrath Les Raisins de la colère à l’écran

Directory of Open Access Journals (Sweden)

Stephen J. Whitfield

2009-07-01

Full Text Available Publié en 1939 et adapté à l’écran l’année suivante, Les Raisins de la colère, roman de John Steinbeck, eut un impact immédiat et extraordinaire pour le portrait sans concession qu’il dressait d’un capitalisme américain qui semblait avoir abandonné sa progéniture la plus loyale, la plus assidue et la plus courageuse, celle qui labourait la terre en plein coeur de la république. Écrit à la toute fin de la « Décennie rouge », mais juste avant que les États-Unis entrent dans la Seconde Guerre mondiale, Les Raisins de la colère exprimaient, d’une certaine manière, la colère accumulée contre un système économique qui avait trahi le prolétariat rural. Le roman, tout comme le film de John Ford (1940, furent compris par le grand public de l’époque, et bien des années plus tard, comme une protestation clairement gauchiste, voire radicale, contre une telle injustice. Mais cet essai montre combien les idées politiques de ces œuvres jumelées sont en réalité difficiles à saisir et combien il est hasardeux de définir, quel que soit l’effort rétrospectif, la critique que Steinbeck et Ford ont proposée dans leur version des Raisins de la colère, une œuvre inoubliable, déchirante mais ambiguë sur le plan politique.

Through-Flow Calculations in Axial Turbomachinery

Science.gov (United States)

1976-10-01

MEETING Dr Ing. G.Winterfeld, DFVLR, Germany PANEL EXECUTIVE Lt. Colonel J.B.Catiller, USAF - Meeting Organisation Dipl. Ing. J.H.Krengel, D.I.G...ralemenc subsonique. Ceci. ne veut d’ailleurs pas dire quo 10 problt~me du passage d’u-ne zone cupersonique A une zone subsonique ( chocs , blocago) se...ph~nom~nes tols que ci~collement, ondes do choc , etc... Nous avons v~rifi6 qu’une force do frottemont oppos~e A la viteese pout rernplir ce role sar.s

DICE/ColDICE: 6D collisionless phase space hydrodynamics using a lagrangian tesselation

Science.gov (United States)

Sousbie, Thierry

2018-01-01

DICE is a C++ template library designed to solve collisionless fluid dynamics in 6D phase space using massively parallel supercomputers via an hybrid OpenMP/MPI parallelization. ColDICE, based on DICE, implements a cosmological and physical VLASOV-POISSON solver for cold systems such as dark matter (CDM) dynamics.

Environmental Impact Statement. Disposal and Reuse of Chanute Air Force Base, Illinois

Science.gov (United States)

1991-07-01

health care and recreation and residential MR. GENE AEFSiY lB 18 use. LT. COL. TO BARTOL 19 19 Additional elements of the proposed MR. POBERT ORR 20 20...an dhan presentied for theutility systemts. 3 The Air Fance will puoipu in dieusap oas eSE ystnniandeailed inw die~mdt&k following individual

Verification of the production of peptide leukotrienes (LT) in traumatic shock

International Nuclear Information System (INIS)

Hock, C.E.; Craft, D.V.; Lefer, D.J.; Lefer, A.M.

1986-01-01

Both lipoxygenase inhibition and leukotriene receptor antagonism have been demonstrated to provide significant protection in traumatic shock. Despite these findings, leukotrienes have not been found in circulating blood in Noble-Collip drum induced traumatic shock using radioimmunoassay techniques. Anesthetized rats subjected to Noble-Collip drum trauma developed a shock state characterized by a significant reduction in mean arterial blood pressure, a 4.5 fold increase in plasma cathepsin D activity, a 3-fold increase in myocardial depressant factor activity and a mean survival time of 1.9 +/- 0.3 hours. Plasma and bile samples were analyzed by reverse phase high pressure liquid chromatography to determine LT production in this shock model. No detectable peptide leukotrienes or their metabolites were found in plasma. The major peptide leukotriene from bile eluted between LTC 4 and LTD 4 and corresponds to a metabolite of LTE 4 , N-acetyl-LTE 4 . This metabolite increased from 6 +/- 3 to 41 +/- 4 units in traumatic shock when compared to sham trauma (p 4 , LTD 4 and LTE 4 (10 μg/kg/h) also resulted in the metabolism of > 90% of the parent LT to this metabolite in bile. Therefore, plasma LTs accumulate in the bile following trauma in rats. Moreover, LTC 4 , LTD 4 and LTE 4 apparently are rapidly metabolized to N-acetyl LTE 4 . These findings further support a role for leukotrienes in the pathogenesis of traumatic shock in rats

Inquérito sobre fungos anemófilos na fronteira Brasil - Colômbia

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Simão Arão Pecher

1988-06-01

Full Text Available Foi realizado um inquérito, sobre fungos anemófllos, em três vilarejos situados na fronteira Brasil-Colômbia, zona muito quente e úmida, durante o mês de julho de 1978. Em apenas uma coleta feita, em diferentes pontos, foram cultivadas colônias pertencentes a dezoito gêneros diferentes de fungos anemófllos, com predominância de Mucor (64,3%, Cândida (54,8%, Rhodotorula (38,1% e Penicillium (38,1%.An inquiry of anemophilous fungi was carried out in small towns located at the Brasilian- Colombian border, a hot and humid zone, during the month of July, 1978. In just one collection, carried out at different sites, colonies were cultivated, and belonged to eighteen different species of anemophilous fungi with predominance of Mucor (64.3%, Candida (54.8%, Rhodotorula (38.1% and Penicillium (38.1%.

Homotopy perturbation method with Laplace Transform (LT-HPM) for solving Lane-Emden type differential equations (LETDEs).

Science.gov (United States)

Tripathi, Rajnee; Mishra, Hradyesh Kumar

2016-01-01

In this communication, we describe the Homotopy Perturbation Method with Laplace Transform (LT-HPM), which is used to solve the Lane-Emden type differential equations. It's very difficult to solve numerically the Lane-Emden types of the differential equation. Here we implemented this method for two linear homogeneous, two linear nonhomogeneous, and four nonlinear homogeneous Lane-Emden type differential equations and use their appropriate comparisons with exact solutions. In the current study, some examples are better than other existing methods with their nearer results in the form of power series. The Laplace transform used to accelerate the convergence of power series and the results are shown in the tables and graphs which have good agreement with the other existing method in the literature. The results show that LT-HPM is very effective and easy to implement.

Kefir Kültürünün Beyaz Peynir Yapımında Kullanılması Üzerine bir Araştırma

Directory of Open Access Journals (Sweden)

Hasan Yaygın

2015-02-01

Full Text Available Beyaz peynir yapımında kullanılacak saf kültürde yer alması gereken mikroorganizmaları saptamak amacı ile bazı çalışmalar yapılmıştır. Araştırıcıların çoğunluğu saf kültürde S. lactis, S. cremoris ve L. casei’ nin bulunması gerektiğini belirtmişlerdir. Bu çalışmada beyaz peynir yapımında saf kültür olarak kefir kültürü kullanılmıştır. Kefir kültüründe söz konusu bakterilerle beraber bazı maya ve bakteriler de bulunmaktadır. Araştırma sonuçları kefir kültürü katılan sütten yapılan beyaz peynirde asitlik gelişiminin istenilen düzeyde olduğu; tat ve aroma ile yapının tüketiciler tarafından beğenildiğini ortaya çıkarmıştır.

Genetic Variations of COL4A1 Gene and Intracerebral Hemorrhage Outcome: A Cohort Study in a Chinese Han Population.

Science.gov (United States)

Xia, Chao; Lin, Sen; Yang, Jie; He, Sha; Li, Hao; Liu, Ming; You, Chao

2018-05-01

To investigate the relationship between single nucleotide polymorphisms or haplotypes of COL4A1 gene and the outcome of intracerebral hemorrhage (ICH). In our study, 181 patients with hypertensive ICH were enrolled and followed up at 3 and 6 months. Outcome data included any cause of death and disability. Genomic DNA was extracted by DNA extraction kit, and the 6 single nucleotide polymorphism genotyping of the COL4A1 gene was detected through MassARRAY Analyzer. Unphased 3.1.4 and SPSS 19.0 were used to analyze the association between alleles, genotypes, and haplotypes of the COL4A1 gene and the outcomes of ICH. Of the 181 patients with hypertensive ICH, 12 were lost in follow-up, which accounted for 6.6%. Our association analysis showed that the rs532625 AA genotype of the COL4A1 gene may increase risk of disability at 3 months; the rs532625 A allele and AA genotype were association factors of the risk of disability at 6 months; the rs532625 AA genotype was an association factor of the risk of death/disability at 6 months. After adjusting for gender, age, coma, and severe neurologic deficits, only the rs532625 AA genotype was independently associated with the risk of disability at 3 and 6 months and the risk of death/disability at 6 months. Our study found that the rs532625 AA genotype in the COL4A1 gene was independently associated with the risk of disability at 3 and 6 months and death/disability at 6 months in a Chinese Han population. These conclusions need to be verified in future studies with larger samples. Copyright © 2018 Elsevier Inc. All rights reserved.

Kültürel Farklılıkların Yönetiminde İnsan Kaynakları Stratejilerinin Rolü : IKEA Örneği

OpenAIRE

Helvacıoğlu, Nuray; Özutku, Hatice

2010-01-01

Günümüzde işletmelerin küreselleşme eğilimleri, işgören çeşitliliğinin ve kültürel farklılıkların artmasına neden olmaktadır. İşletmelerin rekabet üstünlüğü elde edebilmeleri için kültürel farklılıkların etkin bir biçimde yönetilmesi gerekmektedir. Bu çalışmanın amacı, kültürel farklılıkların yönetiminde insan kaynakları yönetiminin rolünün belirlenmesidir. Bu kapsamda, kültürel farklılıkların yönetiminde ön plana çıkan İsveç kökeni IKEA firmasını konu alan nitel bir araş...

Effects of doping on ferroelectric properties and leakage current behavior of KNN-LT-LS thin films on SrTiO3 substrate

Science.gov (United States)

Abazari, M.; Safari, A.

2009-05-01

We report the effects of Ba, Ti, and Mn dopants on ferroelectric polarization and leakage current of (K0.44Na0.52Li0.04)(Nb0.84Ta0.1Sb0.06)O3 (KNN-LT-LS) thin films deposited by pulsed laser deposition. It is shown that donor dopants such as Ba2+, which increased the resistivity in bulk KNN-LT-LS, had an opposite effect in the thin film. Ti4+ as an acceptor B-site dopant reduces the leakage current by an order of magnitude, while the polarization values showed a slight degradation. Mn4+, however, was found to effectively suppress the leakage current by over two orders of magnitude while enhancing the polarization, with 15 and 23 μC/cm2 remanent and saturated polarization, whose values are ˜70% and 82% of the reported values for bulk composition. This phenomenon has been associated with the dual effect of Mn4+ in KNN-LT-LS thin film, by substituting both A- and B-site cations. A detailed description on how each dopant affects the concentrations of vacancies in the lattice is presented. Mn-doped KNN-LT-LS thin films are shown to be a promising candidate for lead-free thin films and applications.

4. FIELU REGI~E~T

African Journals Online (AJOL)

and were absorbed as one complete battery into I Field Brigade under Capt J. L. Gordon-. Gray. After a course at the Military College Maj. Mackenzie was promoted to Lt Col from 1. Feb 1940. Owing to disagreement about se- niorities 1 Field Brigade became 4 Field Bri- gade, comprising: 10 Field Battery, late 1 Field Battery.

Failure to End the Kivu Conflict: Is Rwanda’s Security and Stability at Stake?

Science.gov (United States)

2011-12-16

faculty: Mr. Dave Seigel, Mr. Bill Latham, Mr. Gene King, Dr. Randy Mullis, and Lt Col White. My thanks also go out to my colleagues of Staff Group 2A who...ruled by the “Mafia- 17 type” organizations serving the selfish interests of Mobutu and his entourage, he continues to say. The same view is

Characterization of the Human Proteomic Response to Hydrocodone: A Preliminary Study

Science.gov (United States)

2014-05-01

Bjork, S.J. Grant , Does traumatic brain injury increase risk for substance abuse?, J Neurotrauma (2009). [13] D.P. Graham, A.L. Cardon , An update on...Preliminary Study” 5a. CONTRACT NUMBER 5b. GRANT NUMBER W81XWH-11-2-0126 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Lt Col Vikhyat Bebarta, MD 5d. PROJECT

Marker-Assisted Selection of Xa21 Conferring Resistance to Bacterial Leaf Blight in indica Rice Cultivar LT2

Directory of Open Access Journals (Sweden)

Hue Thi Nguyen

2018-01-01

Full Text Available Bacterial leaf blight of rice (BLB, caused by Xanthomonas oryzae pv. oryzae, is one of the most destructive diseases in Asian rice fields. A high-quality rice variety, LT2, was used as the recipient parent. IRBB21, which carries the Xa21 gene, was used as the donor parent. The resistance gene Xa21 was introduced into LT2 by marker-assisted backcrossing. Three Xoo races were used to inoculate the improved lines following the clipping method. Eleven BC3F3 lines carrying Xa21 were obtained based on molecular markers and agronomic performance. The 11 lines were then inoculated with the three Xoo races. All the 11 improved lines showed better resistance to BLB than the recipient parent LT2. Based on the level of resistance to BLB and their agronomic performance, five lines (BC3F3 5.1.5.1, BC3F3 5.1.5.12, BC3F3 8.5.6.44, BC3F3 9.5.4.1 and BC3F3 9.5.4.23 were selected as the most promising for commercial release. These improved lines could contribute to rice production in terms of food security.

Prolonged exposure of methicillin-resistant Staphylococcus aureus (MRSA) COL strain to increasing concentrations of oxacillin results in a multidrug-resistant phenotype

DEFF Research Database (Denmark)

Martins, Ana; Couto, Isabel; Aagaard, Lone

2007-01-01

Our previous studies demonstrated that exposure of a bacterium to increasing concentrations of an antibiotic would increase resistance to that antibiotic as a consequence of activating efflux pumps. This study utilises the same approach; however, it employs the methicillin-resistant Staphylococcus...... aureus (MRSA) COL strain, which is highly resistant to oxacillin (OXA). MRSA COL was adapted to 3200 mg/L of OXA. Changes in resistance to other antibiotics were evaluated and efflux pump activity during the adaptation process was determined. MRSA COL was exposed to stepwise two-fold increases of OXA....... At the end of each step, minimum inhibitory concentration determination for erythromycin (ERY) and other antibiotics was conducted. Reserpine (RES) was employed to evaluate whether resistance to ERY was dependent on efflux pump activity. Efflux pump activity was also evaluated using the ethidium bromide (EB...

The Continued Need for USAF Light Attack post OEF/OIF: A Survey of West African Infrastructure to Support Tactical Air Operations

Science.gov (United States)

2013-04-01

Mauritania’s two main airfields, at Nouakchott and Atar , have 9,800 foot runways that A-10s could operate from. In addition to those airfields...running road passes through Atar , Zouerat and Bir Moghrein on the way to Algeria. Without the use of the bases at Zouerat and Bir Moghrein the highway...runs almost 400 miles beyond extended duration air support from Atar . With the legacy platforms currently available, the USAF could provide air

A novel splice variant in the N-propeptide of COL5A1 causes an EDS phenotype with severe kyphoscoliosis and eye involvement.

Directory of Open Access Journals (Sweden)

Sofie Symoens

Full Text Available BACKGROUND: The Ehlers-Danlos Syndrome (EDS is a heritable connective tissue disorder characterized by hyperextensible skin, joint hypermobility and soft tissue fragility. The classic subtype of EDS is caused by mutations in one of the type V collagen genes (COL5A1 and COL5A2. Most mutations affect the type V collagen helical domain and lead to a diminished or structurally abnormal type V collagen protein. Remarkably, only two mutations were reported to affect the extended, highly conserved N-propeptide domain, which plays an important role in the regulation of the heterotypic collagen fibril diameter. We identified a novel COL5A1 N-propeptide mutation, resulting in an unusual but severe classic EDS phenotype and a remarkable splicing outcome. METHODOLOGY/PRINCIPAL FINDINGS: We identified a novel COL5A1 N-propeptide acceptor-splice site mutation (IVS6-2A>G, NM_000093.3_c.925-2A>G in a patient with cutaneous features of EDS, severe progressive scoliosis and eye involvement. Two mutant transcripts were identified, one with an exon 7 skip and one in which exon 7 and the upstream exon 6 are deleted. Both transcripts are expressed and secreted into the extracellular matrix, where they can participate in and perturb collagen fibrillogenesis, as illustrated by the presence of dermal collagen cauliflowers. Determination of the order of intron removal and computational analysis showed that simultaneous skipping of exons 6 and 7 is due to the combined effect of delayed splicing of intron 7, altered pre-mRNA secondary structure, low splice site strength and possibly disturbed binding of splicing factors. CONCLUSIONS/SIGNIFICANCE: We report a novel COL5A1 N-propeptide acceptor-splice site mutation in intron 6, which not only affects splicing of the adjacent exon 7, but also causes a splicing error of the upstream exon 6. Our findings add further insights into the COL5A1 splicing order and show for the first time that a single COL5A1 acceptor-splice site

Genetic screening of a pedigree with osteogenesis imperfecta type Ⅰand identification of a novel mutation in COL1A2 pathogenic gene%成骨不全Ⅰ型家系的基因检测和COL1A2基因新突变的致病性鉴定

Institute of Scientific and Technical Information of China (English)

李荣; 郭源平; 潘敬新; 郭奕斌

2015-01-01

To uncover the molecular pathogenic mechanism of congenital osteogenesis imperfecta (OI) type I, all the 103 exons of the COL1A1 (Collagen, type Ⅰ, alpha 1) and COL1A2 (Collagen, type Ⅰ, alpha 2) genes in a child with OI type Ⅰ were screened using PCR-DNA direct sequencing. The results showed no pathological mutation in COL1A1 gene, but a novel mutation c.946G>T/p.G316C in the exon 19 of COL1A2 gene, which was inherited from her father. This mutation was not found in her mother and other six phenotypically normal relatives. By denaturing high perfor-mance liquid chromatography (DHPLC) screening, the abnormal double-peak was visualized in PCR products of exon 19 of COL1A2 gene in the proband and her father, while the normal single-peak was shown in those of her mother and all the healthy controls. Using allele specific amplification (ASA) screening, a specific band of 391 bp in COL1A2 exon 19 was amplified only in the proband and her father, but not in other samples. The amino acid encoded by the mutation site is evolutionarily highly conserved, and this mutation was a“damaging”or“probably damaging”factor to OI type Ⅰ, based on the predicting results using SIFT and Polyphen-2 softwares. In conclusion, the novel c.946G>T/p.G316C mutation in COL1A2 gene is a pathogenic mutation that could result in OI type Ⅰ. If the couple wants to get pregnant again, it is necessary to screen the mutation site in COL1A2 gene through the prenatal genetic diagnosis in the first trimester or through preimplantation genetic diagnosis (PGD) in the progestation.%为了揭示成骨不全(Osteogenesis imperfecta, OI)Ⅰ型家系的分子遗传学发生机制，文章采用PCR-DNA直接测序法，对患儿 COL1A1和 COL1A2基因共103个外显子(E)进行突变检测。结果显示：患儿 COL1A1基因未发现任何病理性突变，而在 COL1A2基因 E19内发现一新的杂合错义突变(p.G316C)，该突变来自其父，而其母正常，其他表型正常的6�

Two-step method for curing Escherichia coli of ColE1-derived plasmids

DEFF Research Database (Denmark)

Hove-Jensen, Bjarne

2008-01-01

To cure Escherichia coli for plasmids derived from the ColE1 replicon advantage is taken of the fact that maintenance of this replicon requires a wild-type allele of polA, encoding DNA polymerase I. Curing is achieved by cotransduction of a mutant polA allele with metE::Tn10, fadAB::Tn10 or other...

Investigando a construção de sentidos sobre o ambiente em visitas de crianças a um colégio agrícola

OpenAIRE

Arante, Juliana de Souza Neves

2009-01-01

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Educação Científica e Tecnológica, Florianópolis, 2009 É prática comum, no Colégio Agrícola Senador Carlos Gomes de Oliveira (CASCGO), localizado no município de Araquari - SC, as visitas programadas de turmas de outros colégios para que conheçam como funciona uma escola-fazenda. Para que esta atividade ocorra, o colégio conta com seus próprios alunos como guias dessas visitas. O objetivo principa...

Análise da expressão do colágeno VI na distrofia muscular congênita Analysis of the expression of collagen VI in congenital muscular dystrophy

Directory of Open Access Journals (Sweden)

Regina Toni Loureiro de Freitas

2005-06-01

Full Text Available A distrofia muscular congênita (DMC compõe um grupo de miopatias caracterizadas por hipotonia e fraqueza muscular notadas já no primeiro ano de vida. A forma de Ullrich é caracterizada por retrações musculares proximais e hiperextensibilidade distal. Cerca de 40% destes pacientes apresentam mutações em um dos genes que codificam as três sub-unidades do colágeno VI (COL6, acarretando deficiência total ou parcial na marcação da proteína. Analisamos, através de imunofluorescência, a marcação do COL6 em fragmentos musculares de 50 pacientes com DMC, 20 deles com ausência da marcação para merosina. Identificamos 4 casos com deficiência total da marcação do COL6 (8% do total, representando 13% dos casos com marcação normal para merosina. As alterações histológicas musculares dos pacientes com COL6 deficiente eram indistinguíveis das outras formas de DMC, porém mais brandas que as observadas na DMC com deficiência de merosina. Em três dos pacientes com COL6 deficiente observou-se hipotonia e fraqueza muscular, notadas já no período neonatal, atraso do desenvolvimento motor, retrações musculares em joelhos e cotovelos, hiperextensibilidade distal e luxação congênita do quadril (dois pacientes. Um paciente perdeu a capacidade para a marcha, e outro faleceu por problemas respiratórios. A análise da marcação do COL6, assim como da merosina, no tecido muscular de pacientes com DMC pode auxiliar na identificação e caracterização fenotípica dos diversos subtipos de DMC.Congenital muscular dystrophy (CMD composes a group of disorders characterized by hypotonia and muscular weakness noticed in the first year of life. The Ullrich's form is characterized by proximal joint contractures and distal hiperextensibility. About 40% of these patients present mutations in one of the genes that codify the sub-units of the collagen VI protein (COL6, producing total or partial deficiency of the protein expression. We analyzed

Composición química del huevo de Tortuga Golfina Lepidochelys olivacea (Testudines: Cheloniidae y su potencial como recurso alimenticio

Directory of Open Access Journals (Sweden)

María Isabel Castro-González

2011-12-01

Full Text Available Por su potencial como fuente alimenticia, se analizó la composición química de huevo de Lepidochelys olivácea en La Escobilla, Oaxaca, México. El Instituto de Ciencias del Mar y Limnología de la Universidad Nacional Autónoma de México proporcionó 250 muestras de huevo liofilizado de 25 tortugas, que se analizaron siguiendo métodos oficiales para humedad, cenizas, proteína, grasa, lípidos totales(LT y análisis microbiológicos; además de ácidos grasos(AG por cromatografía de gases, aminoácidos(AA, vitaminas y colesterol(col por HPLC. Los resultados fueron los siguientes: (g/100g humedad(4.7, cenizas(3.8, proteína(53.7 y grasa(47.4. AA esenciales (g aa/100g Proteína: Ile (4.4, Lys(6.6, Leu(7.4, Met+Cys (8.8, Phe+Tyr (10.8; retinol (340μg/100g, colecalciferol (5.9 μg/100g, tocoferol (8.6, tiamina (0.3 y riboflavina (1.1 (mg/100g. Y los de (LT, (AG y (Col se concentraron en tres grupos por peso de tortuga: (LT (44.3-48.7-49.1g/100g, (Col (518.4-522.5-728.7 mg/100g. Entonces se identificaron 17 AGSaturados, 8 AGMonoinsaturados y 11 AGPoliinsaturados. Los AGS más abundantes (mg/100g: C16:0 (485-1263, AGM: C16:1 (456-716, C18:1n-9c (904- 1754 y AGP: C20:4n-6 (105-217, EPA (48-103 y HA (97-189. También, existió diferencia significativa (Fisher, p<0.05 para (Col, AG totales, AGS, AGM, AGP y AG n-3 (EPA+DHA. No se detectó presencia de agentes microbiológicos. El huevo liofilizado de L. olivacea es una opción para el desarrollo de productos alimenticios por su proteína de alto valor biológico y ácidos grasos n-3.

Carcinome à cellule vitreuse du col de l'utérus: à propos d'un cas et revue de littérature

Science.gov (United States)

Hakimi, Ihssane; Zazi, Abdelghani; Chahdi, Hafsa; Guelzim, Khalid; Kouach, Jaouad; Babahabib, Myabdellah; Elhassani, Myehdi; Rahali, Driss Moussaoui; Dehayni, Mohammed

2015-01-01

Le carcinome à cellule vitreuse du col de l'utérus est un type de histologique rare de cancer du col de l'utérus qui survient à un âge plus jeune, et s'associe au risque élevé d’échec thérapeutique et le pronostic est plus mauvais en comparaison au type cellulaire squameux. La radiothérapie est associée au risque diminué de récidive. Le but de cette étude est de récapituler à travers d'une observation et une revue de littérature les données sur l'incidence, le comportement clinique et la survie globale de patients avec le carcinome à cellule vitreuse du col de l'utérus. PMID:26664556

Views of Astronaut (Col.) Joe Engle and son Jon with L-5 Piper Cub

Science.gov (United States)

1981-01-01

Views of Astronaut (Col.) Joe Engle and son Jon with L-5 Piper Cub at Clover Airport. Photos include Engle turning propeller while his son sits in the cockpit (34323); both Engle and son examine propeller (34324); Engle works on engine while his son sits in cockpit (34325).

Inter-Accions. Una experiència transdisciplinària en un context pedagògic col·lectiu

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Marta Carrasco-Bonet

2015-07-01

Full Text Available Inter-Accions és un projecte pedagògic col·lectiu interuniversitari que treballa des de l'autogestió i la transdisciplinarietat art-arquitectura. És un projecte d’estudiants i per als estudiants d’aquests dos camps que es proposa davant la necessitat d’obrir espais autònoms d’aprenentatge dins la mateixa institució. L’objectiu principal de la proposta és construir un espai d’experimentació on sigui possible treballar sobre la gestió col·lectiva de l’aprenentatge més enllà dels continguts i els resultats. Des d’una perspectiva crítica i en un context acadèmic es proposa aquesta experiència per tal que arquitectes i artistes comparteixin rols i que, a partir del treball col·lectiu i col·laboratiu basat en la idea de codi obert, arribin a establir noves metodologies útils, pràctiques i crítiques aplicables al desenvolupament del seu treball sobre la ciutat. Els projectes que s’han desenvolupat en el marc dels diferents tallers prenen la ciutat com a espai de reflexió i d’intervenció, i permeten arribar a la conclusió que ha estat més determinant l’interès en els mètodes i processos d’aproximació i l’interès en els processos de creació i d’aprenentatge que la voluntat d’aconseguir resultats finalistes. Aquest projecte s’està desenvolupant avui en el marc d’una Assignatura d’Extensió Universitària anomenada “Cartografia Artística” a la Universitat de Barcelona.

Auto-imunidade e colágeno V Autoimmunity and collagen V

Directory of Open Access Journals (Sweden)

Cristiane Carla de Oliveira

2006-06-01

Full Text Available As proteínas da matriz extracelular (MEC e seus componentes estão sendo amplamente estudados na literatura médica, assim como sua relação com o remodelamento tecidual presente nas doenças reumáticas. Neste artigo, mostramos a importância do estudo do colágeno do tipo V no entendimento da etiologia da esclerodermia, no que se refere ao desencadeamento da auto-imunidade nesta enfermidade. Estudos em nosso laboratório demonstram que a sensibilização com colágeno do tipo V em coelhos pode resultar em um modelo animal de esclerodermia. Diante destes fatos, sugerimos que pesquisas neste campo podem ser de grande valia no desenvolvimento de novas condutas terapêuticas.The extracellular matrix (ECM proteins and their components have been widely studied in medical literature, as well its relationship with the tecidual remodeling present in the rheumatic disease. In this paper we show the importance of understanding the role of type V collagen as an important trigger of rheumatic autoimmune diseases. Studies in our laboratory demonstrate that type V collagen sensibilization in rabbits, could result in an animal scleroderma model. In this way we suggested that researches in this field can be worthy in development of new therapeutic procedures.

Identification of a umuDC locus in Salmonella typhimurium LT2

International Nuclear Information System (INIS)

Smith, C.M.; Eisenstadt, E.

1989-01-01

The umuDC operon of Escherichia coli is required for efficient mutagenesis by UV light and many other DNA-damaging agents. The existence of a umuDC analog in Salmonella typhimurium has been questioned. With DNA probes to the E. coli umuD and umuC genes, we detected, by Southern blot hybridization, sequences similar to both of these genes in S. typhimurium LT2. We also confirmed that the presence of cloned E. coli umuD enhances the UV mutability and resistance of S. typhimurium. Our data strongly suggest that S. typhimurium contains a functional umuDC operon

Demarcação e Divulgação dos Limites entre o Brasil e a Colômbia

OpenAIRE

Novaes, André Reyes; Palacios, David Ramírez

2015-01-01

Os documentos aqui reproduzidos expressam um momento importante no processo de demarcação dos limites entre o Brasil e a Colômbia. O primeiro é a “Acta da vigésima primeira e última conferência da Comissão Mixta Brasileira Colombiana Demarcadora de Limites”, realizada em 5 de janeiro de 1937 na cidade de Manaus. O segundo é a “Carta geográfica da fronteira do Alto Solimões entre Brasil, Perú e Colômbia”, publicada em 1940 com base nos trabalhos da citada comissão, e disponível na Biblioteca D...

The generation and characterization of novel Col1a1FRT-Cre-ER-T2-FRT and Col1a1FRT-STOP-FRT-Cre-ER-T2 mice for sequential mutagenesis

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Minsi Zhang

2015-09-01

Full Text Available Novel genetically engineered mouse models using the Cre-loxP or the Flp-FRT systems have generated useful reagents to manipulate the mouse genome in a temporally-regulated and tissue-specific manner. By incorporating a constitutive Cre driver line into a mouse model in which FRT-regulated genes in other cell types are regulated by Flp-FRT recombinase, gene expression can be manipulated simultaneously in separate tissue compartments. This application of dual recombinase technology can be used to dissect the role of stromal cells in tumor development and cancer therapy. Generating mice in which Cre-ERT2 is expressed under Flp-FRT-mediated regulation would enable step-wise manipulation of the mouse genome using dual recombinase technology. Such next-generation mouse models would enable sequential mutagenesis to better model cancer and define genes required for tumor maintenance. Here, we generated novel genetically engineered mice that activate or delete Cre-ERT2 in response to Flp recombinase. To potentially utilize the large number of Cre-loxP-regulated transgenic alleles that have already been targeted into the Rosa26 locus, such as different reporters and mutant genes, we targeted the two novel Cre-ERT2 alleles into the endogenous Col1a1 locus for ubiquitous expression. In the Col1a1FRT-Cre-ER-T2-FRT mice, Flp deletes Cre-ERT2, so that Cre-ERT2 is only expressed in cells that have never expressed Flp. In contrast, in the Col1a1FRT-STOP-FRT-Cre-ER-T2 mice, Flp removes the STOP cassette to allow Cre-ERT2 expression so that Cre-ERT2 is only expressed in cells that previously expressed Flp. These two new novel mouse strains will be complementary to each other and will enable the exploration of complex biological questions in development, normal tissue homeostasis and cancer.

The generation and characterization of novel Col1a1FRT-Cre-ER-T2-FRT and Col1a1FRT-STOP-FRT-Cre-ER-T2 mice for sequential mutagenesis

Science.gov (United States)

Zhang, Minsi; Kirsch, David G.

2015-01-01

ABSTRACT Novel genetically engineered mouse models using the Cre-loxP or the Flp-FRT systems have generated useful reagents to manipulate the mouse genome in a temporally-regulated and tissue-specific manner. By incorporating a constitutive Cre driver line into a mouse model in which FRT-regulated genes in other cell types are regulated by Flp-FRT recombinase, gene expression can be manipulated simultaneously in separate tissue compartments. This application of dual recombinase technology can be used to dissect the role of stromal cells in tumor development and cancer therapy. Generating mice in which Cre-ERT2 is expressed under Flp-FRT-mediated regulation would enable step-wise manipulation of the mouse genome using dual recombinase technology. Such next-generation mouse models would enable sequential mutagenesis to better model cancer and define genes required for tumor maintenance. Here, we generated novel genetically engineered mice that activate or delete Cre-ERT2 in response to Flp recombinase. To potentially utilize the large number of Cre-loxP-regulated transgenic alleles that have already been targeted into the Rosa26 locus, such as different reporters and mutant genes, we targeted the two novel Cre-ERT2 alleles into the endogenous Col1a1 locus for ubiquitous expression. In the Col1a1FRT-Cre-ER-T2-FRT mice, Flp deletes Cre-ERT2, so that Cre-ERT2 is only expressed in cells that have never expressed Flp. In contrast, in the Col1a1FRT-STOP-FRT-Cre-ER-T2 mice, Flp removes the STOP cassette to allow Cre-ERT2 expression so that Cre-ERT2 is only expressed in cells that previously expressed Flp. These two new novel mouse strains will be complementary to each other and will enable the exploration of complex biological questions in development, normal tissue homeostasis and cancer. PMID:26183214

A New COL3A1 Mutation in Ehlers-Danlos Syndrome Vascular Type With Different Phenotypes in the Same Family.

Science.gov (United States)

Cortini, Francesca; Marinelli, Barbara; Romi, Silvia; Seresini, Agostino; Pesatori, Angela Cecilia; Seia, Manuela; Montano, Nicola; Bassotti, Alessandra

2017-04-01

Vascular Ehlers-Danlos syndrome (vEDS) is a rare and severe connective tissue disorder caused by mutations in the collagen type III alpha I chain ( COL3A1) gene. We describe a pathogenetic heterozygous COL3A1 mutation c.3140 G>A, p. Gly1047Asp, identified using next-generation sequencing, in a 40-year-old Italian female. The genetic test performed on her relatives, which present different clinical phenotypes, confirmed that they carry the same mutation in heterozygous state. This finding confirms that mutations causing vEDS have an incomplete penetrance.

A novel nonsteroidal antifibrotic oligo decoy containing the TGF-beta element found in the COL1A1 gene which regulates murine schistosomiasis liver fibrosis.

Science.gov (United States)

Boros, D L; Singh, K P; Gerard, H C; Hudson, A P; White, S L; Cutroneo, K R

2005-08-01

Schistosomiasis mansoni disseminated worm eggs in mice and humans induce granulomatous inflammations and cumulative fibrosis causing morbidity and possibly mortality. In this study, intrahepatic and I.V. injections of a double-stranded oligodeoxynucleotide decoy containing the TGF-beta regulatory element found in the distal promoter of the COL1A1 gene into worm-infected mice suppressed TGF-beta1, COL1A1, tissue inhibitor of metalloproteinase-1, and decreased COL3A1 mRNAs to a lesser extent. Sequence comparisons within the mouse genome found homologous sequences within the COL3A1, TGF-beta1, and TIMP-1 5' flanking regions. Cold competition gel mobility shift assays using these homologous sequences with 5' and 3' flanking regions found in the natural COL1A1 gene showed competition. Competitive gel mobility assays in a separate experiment showed no competition using a 5-base mutated or scrambled sequence. Explanted liver granulomas from saline-injected mice incorporated 10.45 +/- 1.7% (3)H-proline into newly synthesized collagen, whereas decoy-treated mice showed no collagen synthesis. Compared with the saline control schistosomiasis mice phosphorothioate double-stranded oligodeoxynucleotide treatment decreased total liver collagen content (i.e. hydroxy-4-proline) by 34%. This novel molecular approach has the potential to be employed as a novel antifibrotic treatment modality. (c) 2005 Wiley-Liss, Inc.

Loss of col8a1a Function during Zebrafish Embryogenesis Results in Congenital Vertebral Malformations

Science.gov (United States)

Gray, Ryan S.; Wilm, Thomas; Smith, Jeff; Bagnat, Michel; Dale, Rodney M.; Topczewski, Jacek; Johnson, Stephen L.; Solnica-Krezel, Lilianna

2014-01-01

Congenital vertebral malformations (CVM) occur in 1 in 1,000 live births and in many cases can cause spinal deformities, such as scoliosis, and result in disability and distress of affected individuals. Many severe forms of the disease, such as spondylocostal dystostosis, are recessive monogenic traits affecting somitogenesis, however the etiologies of the majority of CVM cases remain undetermined. Here we demonstrate that morphological defects of the notochord in zebrafish can generate congenital-type spine defects. We characterize three recessive zebrafish leviathan/col8a1a mutant alleles (m531, vu41, vu105) that disrupt collagen type VIII alpha1a (col8a1a), and cause folding of the embryonic notochord and consequently adult vertebral column malformations. Furthermore, we provide evidence that a transient loss of col8a1a function or inhibition of Lysyl oxidases with drugs during embryogenesis was sufficient to generate vertebral fusions and scoliosis in the adult spine. Using periodic imaging of individual zebrafish, we correlate focal notochord defects of the embryo with vertebral malformations (VM) in the adult. Finally, we show that bends and kinks in the notochord can lead to aberrant apposition of osteoblasts normally confined to well-segmented areas of the developing vertebral bodies. Our results afford a novel mechanism for the formation of VM, independent of defects of somitogenesis, resulting from aberrant bone deposition at regions of misshapen notochord tissue. PMID:24333517

USAF Flight Test Investigation of Focused Sonic Booms: Project Have Bears

Science.gov (United States)

Downing, Micah; Zamot, Noel; Moss, Chris; Morin, Daniel; Wolski, Ed; Chung, Sukhwan; Plotkin, Kenneth; Maglieri, Domenic

1996-01-01

Supersonic operations from military aircraft generate sonic booms that can affect people, animals and structures. A substantial experimental data base exists on sonic booms for aircraft in steady flight and confidence in the predictive techniques has been established. All the focus sonic boom data that are in existence today were collected during the 60's and 70's as part of the information base to the US Supersonic Transport program and the French Jericho studies for the Concorde. These experiments formed the data base to develop sonic boom propagation and prediction theories for focusing. There is a renewed interest in high-speed transports for civilian application. Moreover, today's fighter aircraft have better performance capabilities, and supersonic flights ars more common during air combat maneuvers. Most of the existing data on focus booms are related to high-speed civil operations such as transitional linear accelerations and mild turns. However, military aircraft operating in training areas perform more drastic maneuvers such as dives and high-g turns. An update and confirmation of USAF prediction capabilities is required to demonstrate the ability to predict and control sonic boom impacts, especially those produced by air combat maneuvers.

WHAT MIGHT HAVE BEEN

African Journals Online (AJOL)

and In 1941which gave rise to this fascinating forum article by Lt Col A. G. .... twelve on her own, Hitler, in a megalomaniacal gesture, invaded ... with an equally magniloquent gesture, called. Russia a friend and ally, and pledged the British. Empire to give her all the aid and help that lay within its power. And this pledge it ...

Trace fossils from the eocene Lillebælt clay formation, Røsnæs Peninsula, Denmark

DEFF Research Database (Denmark)

Nielsen, Jan Kresten; Milàn, Jesper; Mesfun, Daniel

2015-01-01

A cliff exposure of the Eocene Lillebælt Clay Formation, on the Røsnæs peninsula of Zealand, Denmark, has yielded a diverse trace-fossil assemblage. The trace fossils are described formally for the first time and assigned to Phymatoderma melvillensis, unnamed clusters of small burrows, Ophiomorpha...

Over-expression of DSCAM and COL6A2 cooperatively generates congenital heart defects.

Directory of Open Access Journals (Sweden)

Tamar R Grossman

2011-11-01

Full Text Available A significant current challenge in human genetics is the identification of interacting genetic loci mediating complex polygenic disorders. One of the best characterized polygenic diseases is Down syndrome (DS, which results from an extra copy of part or all of chromosome 21. A short interval near the distal tip of chromosome 21 contributes to congenital heart defects (CHD, and a variety of indirect genetic evidence suggests that multiple candidate genes in this region may contribute to this phenotype. We devised a tiered genetic approach to identify interacting CHD candidate genes. We first used the well vetted Drosophila heart as an assay to identify interacting CHD candidate genes by expressing them alone and in all possible pairwise combinations and testing for effects on rhythmicity or heart failure following stress. This comprehensive analysis identified DSCAM and COL6A2 as the most strongly interacting pair of genes. We then over-expressed these two genes alone or in combination in the mouse heart. While over-expression of either gene alone did not affect viability and had little or no effect on heart physiology or morphology, co-expression of the two genes resulted in ≈50% mortality and severe physiological and morphological defects, including atrial septal defects and cardiac hypertrophy. Cooperative interactions between DSCAM and COL6A2 were also observed in the H9C2 cardiac cell line and transcriptional analysis of this interaction points to genes involved in adhesion and cardiac hypertrophy. Our success in defining a cooperative interaction between DSCAM and COL6A2 suggests that the multi-tiered genetic approach we have taken involving human mapping data, comprehensive combinatorial screening in Drosophila, and validation in vivo in mice and in mammalian cells lines should be applicable to identifying specific loci mediating a broad variety of other polygenic disorders.

Tüketim kültüründe etkili bir araç olarak reklamın işlevlerine dair genel bir değerlendirme

OpenAIRE

Elmasoğlu, Kamile

2017-01-01

Betimsel analiz yönteminin kullanıldığı bu çalışmada, tüketim kültürünün yayılmasında etkili bir araç olarak görülen reklamın rolüne dair genel bir değerlendirme sunmak hedeflenmektedir. Bu hedef dâhilinde, tüketim toplumu, tüketim kültürü ve reklamın tüketim kültürü içindeki işlevleri; çeşitli düşünürlerin görüşlerinden istifade edilerek tartışılmıştır. Tüketim kültürünün hakim olduğu bir toplumda reklama ilişkin çeşitli fikirlerin benimsendiği görülmektedir. Çalışma genel olarak değerlendir...

Quantum-well exciton dipolar interaction: Polarization-dependence and Z-LT splitting

International Nuclear Information System (INIS)

Nguyen Ba An.

1996-12-01

We calculate the exciton dipolar interaction in a semiconductor quantum well. The explicit polarization-dependence, i.e, the dependence on both the exciton dipole moment μ-vector and its inplane wavevector k-vector is derived. The obtained results for the three modes (L, T and Z modes) of the long-range part of the dipolar interaction satisfy the polarization sum rule for any parameters. In the long wavelength limit there is a Z-LT splitting which decreases as the well width increases reflecting a crossover from strict 2D to quasi-2D. A rough crossover from quasi-2D to 3D is also described. (author). 18 refs, 4 figs

Käyttäjien tuottama sisältö markkinoinnissa : Opas

OpenAIRE

Koskenseppä, Mikko

2016-01-01

Markkinoijien ja kuluttajien roolit ovat muuttunut 2000-luvun aikana, jonka takia yritykset eivät voi enää pitää markkinointia yksisuuntaisena viestintänä. Nykyään yrityksien pitää saada kuluttajat mukaan markkinointiin, ja käyttäjien tuottama sisältö onkin yksi ratkaisu tähän ongelmaan. Tämä opinnäytetyö oli toiminnallinen työ. Sen tavoitteena oli luoda ohjeistus käyttäjien tuottaman sisällön käyttämisestä markkinoinnissa. Työn muut tavoitteet oli selvittää sen tuomat hyödyt ja ominaisuu...

PREFACE: Proceedings of the 25th International Conference on Low Temperature Physics (LT25) (Amsterdam, The Netherlands, 6-13 August 2008) Proceedings of the 25th International Conference on Low Temperature Physics (LT25) (Amsterdam, The Netherlands, 6-13 August 2008)

Science.gov (United States)

Kes, Peter; Jochemsen, Reijer

2009-04-01

This issue forms part I of the Proceedings of the 25th International Conference on Low Temperature Physics (LT25) (Amsterdam, The Netherlands, 67-13 August 2008). The majority of the special invited lectures, such as the London prize lectures, the international union of pure and applied physics (IUPAP) young scientist award lectures, the plenary, half-plenary and public lectures, and the historical lectures presented at the LT25 conference, are included. The papers relating to the oral and poster presentations will appear in part II of the proceedings in a dedicated open access issue of Journal of Physics: Conference Series (2009 J. Phys.: Conf. Ser. 150). In addition to the organizer's report and a summary of the new developments in low temperature physics, which can also be found in this issue, part II provides useful information about LT25, such as an overview of committees, sponsors, exhibitors, and some conference statistics. To ensure the high publication standard mandated by Journal of Physics: Condensed Matter and Journal of Physics: Conference Series every paper was reviewed by at least one referee before it was accepted for publication. The editors are indebted to many colleagues for invaluable assistance in the preparation and review of 900 papers appearing in both parts I and II of these proceedings. In particular, we would like to thank Carlo Beenakker, Jeroen van den Brink, Hans Brom, Jos de Jongh, Horst Rogalla, Fons de Waele, and Jan Zaanen.

USA kohus nõuab LHV-lt, Lepikult ja Peegilt kiiresti ligi 14 miljonit krooni / Lauri Matsulevitsh

Index Scriptorium Estoniae

Matsulevitsh, Lauri

2005-01-01

USA kohus kinnitas investeerimispangaga LHV eelkokkuleppe ning muutis esialgset hagi tagamise määrust, millega nõuab LHV-lt sealt välja viidud raha USA kohturegistri kontole tagasi viie päeva jooksul. Lisa: Kontodelt nõutakse 13,8 mln krooni. Vt. samas: Advokaat: SEC kasutab jõupositsiooni

Osteogenesis imperfecta Type IV: a newly identified variant at position c.560 (G > T; p.Gly187Val) in the COL1A2 gene.

Science.gov (United States)

Usta, Akin; Karademir, Dilay; Sen, Eylem; Yazici, Selcuk; Adali, Ertan; Erdem, Erkan; Karacan, Meric

2017-01-01

Osteogenesis imperfecta is a clinically heterogenous disease caused by defective collagen syntesis associated with a mutation in the COL1A1 or COL1A2 genes. In this report, we present a case of osteogenesis imperfecta (OI) type IV, seen in a female fetus with incurved femurs at 18 weeks of gestation. Molecular analysis of the newborn revealed a novel mutation at position c.560 (c.560 G > T) of the exon 12 in the COL1A2 gene; which lead to the glycine modification with valine (p.Gly187Val) at codon 187. The pregnancy follow-up was uneventful. After delivery, the newborn underwent biphosponat therapy and no fracture was detected until 1 year old.

Resuscitation of the viable but non-culturable state of Salmonella enterica serovar Oranienburg by recombinant resuscitation-promoting factor derived from Salmonella Typhimurium strain LT2.

Science.gov (United States)

Panutdaporn, N; Kawamoto, K; Asakura, H; Makino, S-I

2006-02-15

A gene encoding the resuscitation-promoting factor (Rpf) from Salmonella Typhimurium LT2 was cloned and characterized. The amino acid sequence encoded by S. Typhimurium LT2 rpf gene shares 24.2% homology with Micrococcus luteus Rpf, which is secreted by growing cells, and required to resuscitate from viable but non-culturable (VNC) state. The S. Typhimurium LT2 rpf gene is 696 bp long, and shared a conserved segment with Salmonella enterica serovar Oranienburg (99.4%). Recombinant Rpf (rRpf) proteins of S. Typhimurium LT2 after expression in E. coli BL21 harboring the pET15-b plasmid was approximately 25 kDa. Since S. Oranienburg cells are relatively quick to enter the VNC state just after incubating in the presence of 7% NaCl at 37 degrees C for 3 days, we evaluated the biological effect of rRpf by using S. Oranienburg VNC cells. The rRpf not only promoted proliferation but also induced resuscitation of VNC cells to the culturable state in a dose-dependent manner. Therefore, rRpf may be useful for detection of bacterial contaminants present in the VNC form in food samples and the environment.

Horizontal gene transfer of a ColV plasmid has resulted in a dominant avian clonal type of Salmonella enterica serovar Kentucky.

Directory of Open Access Journals (Sweden)

Timothy J Johnson

Full Text Available Salmonella enterica continues to be a significant cause of foodborne gastrointestinal illness in humans. A wide variety of Salmonella serovars have been isolated from production birds and from retail poultry meat. Recently, though, S. enterica subsp. enterica serovar Kentucky has emerged as one of the prominent Salmonella serovars isolated from broiler chickens. Recent work suggests that its emergence apparently coincides with its acquisition of a ColV virulence plasmid. In the present study, we examined 902 Salmonella isolates belonging to 59 different serovars for the presence of this plasmid. Of the serovars examined, the ColV plasmid was found only among isolates belonging to the serovars Kentucky (72.9%, Typhimurium (15.0% and Heidelberg (1.7%. We demonstrated that a single PFGE clonal type of S. Kentucky harbors this plasmid, and acquisition of this plasmid by S. Kentucky significantly increased its ability to colonize the chicken cecum and cause extraintestinal disease. Comparison of the completed sequences of three ColV plasmids from S. Kentucky isolated from different geographical locales, timepoints and sources revealed a nearly identical genetic structure with few single nucleotide changes or insertions/deletions. Overall, it appears that the ColV plasmid was recently acquired by a single clonal type S. Kentucky and confers to its host enhanced colonization and fitness capabilities. Thus, the potential for horizontal gene transfer of virulence and fitness factors to Salmonella from other enteric bacteria exists in poultry, representing a potential human health hazard.

Circular RNA profiling reveals that circCOL3A1-859267 regulate type I collagen expression in photoaged human dermal fibroblasts

International Nuclear Information System (INIS)

Peng, Yating; Song, Xiaojing; Zheng, Yue; Wang, Xinyi; Lai, Wei

2017-01-01

Production of type I collagen declines is a main characteristic during photoaging, but the mechanism is still not fully understood. Circular RNAs (circRNAs) are a class of newly identified non-coding RNAs with regulatory potency by sequestering miRNAs like a sponge. It's more stable than linear RNAs, and would be a useful tool for regulation of gene expression. However, the role of circRNAs in collagen expression during photoaging is still unclear. Here we performed deep sequencing of RNA generated from UVA irradiated and no irradiated human dermal fibroblasts (HDFs) and identified 29 significantly differentially expressed circRNAs (fold change ≥ 1.5, P < 0.05), 12 circRNAs were up-regulated and 17 circRNAs were down-regulated.3 most differentially expressed circRNAs were verified by qRT-PCR and the down-regulated circCOL3A1-859267 exhibited the most significantly altered in photoaged HDFs. Overexpression of circCOL3A1-859267 inhibited UVA-induced decrease of type I collagen expression and silencing of it reduced type I collagen intensity. Via a bioinformatic method, 44 miRNAs were predicted to binding with circCOL3A1-859267, 5 of them have been confirmed or predicted to interact with type I collagen. This study show that circCOL3A1-859267 regulate type I collagen expression in photoaged HDFs, suggesting it may be a novel target for interfering photoaging.

Papel del colágeno VI en la homeostasis del músculo esquelético y tejido adiposo: implicaciones en la patofisiología de las distrofias musculares

OpenAIRE

Rodríguez García, Mª Ángeles

2016-01-01

[spa] El colágeno VI es una proteína de la matriz extracelular cuyos defectos conducen a una variedad de trastornos musculares en humanos caracterizados por una debilidad muscular entre otros múltiples síntomas. Tanto el colágeno VI, como su fragmento soluble carboxilo terminal llamado endotrofina se encuentran altamente expresados en los dos principales tejidos periféricos que regulan la glucemia, el músculo esquelético y tejido adiposo. Los niveles de ARNm de colágeno VI en el tejido adipos...

Türk Sucuklarında Ticari Starter Kültür Kullanımı Üzerine Araştırmalar II. Duyusal ve Mikrobiyolojik Analizler

Directory of Open Access Journals (Sweden)

Halil Vural

2015-02-01

Full Text Available Bu çalışmada; işletme düzeyinde kontrollü koşullarda Türk sucuğu üretiminde ticari starter kültürlerin kullanımı incelenmiştir. Bu amaçla, starter kültür katılmayan örnek kontrol grubu olarak alınarak, 7 farklı ticari starter kültürle hazırlanan 8 sucuk örneği aynı koşullarda denemeye alınmıştır. Sucuklara üretimin 7 kritik noktasına mezofilik aerobik bakteri, Lactobacillus-Pedicoccus ve patojen olmayan Staphylococcus-Micrococcus sayımı analizleri ile ürün bazında duyusal analizlerle starter kültür katılarak üretilen sucukların kontrol grubuna göre belirgin üstünlük sağladıkları belirlenmiş, kimyasal, mikrobiyolojik ve duyusal analiz bulgularının birlikte değerlendirilmesi; Türk sucuğu özelliklerine en uygun sonuçları Flora Carn SL ve Flora Carn SP starter kültürleri ile yapılan sucukların verdiğini göstermiştir.

The effects of LT-SN on energy dissipation and lifetime in wireless sensor networks

Directory of Open Access Journals (Sweden)

Zeydin Pala

2016-11-01

Full Text Available Wireless sensor networks (WSNs still attract the attention of researchers, users and the private sector despite their low power and low range tendency for malfunction. This attraction towards WSNs results from their low cost structure and the solutions they offer for many prevalent problems. Many conditions, which remain unforeseen or unexpected during the design of the system, may arise after the initialization of the system. Similarly, many situations where security vulnerabilities take place may emerge in time in WSNs operating normally. In this study, we called nodes which enter sleeping mode without any further waking up and causing a sparser number of nodes in the network without any function in data transmission as Long-Term Sleep Nodes (LT-SN; and considered energy spaces caused by such nodes as a problem; and established two Linear Programming (LP models based on the efficiency of the present nodes. We offered two different models which present the effect of sensor nodes, which were initially operating in wireless sensor network environment and did not wake up following sleep mode, on network lifetime. The results of the present study report that as the number of LT-SN increases, the lifetime of the network decreases.

Celecoxib and tauro-ursodeoxycholic acid co-treatment inhibits cell growth in familial adenomatous polyposis derived LT97 colon adenoma cells

Energy Technology Data Exchange (ETDEWEB)

Heumen, Bjorn W.H. van, E-mail: b.vanheumen@mdl.umcn.nl [Department of Gastroenterology and Hepatology, Radboud University Nijmegen Medical Centre, Nijmegen (Netherlands); Roelofs, Hennie M.J.; Morsche, Rene H.M. te [Department of Gastroenterology and Hepatology, Radboud University Nijmegen Medical Centre, Nijmegen (Netherlands); Marian, Brigitte [Institute of Cancer Research, Wien University, Vienna (Austria); Nagengast, Fokko M.; Peters, Wilbert H.M. [Department of Gastroenterology and Hepatology, Radboud University Nijmegen Medical Centre, Nijmegen (Netherlands)

2012-04-15

Chemoprevention would be a desirable strategy to avoid duodenectomy in patients with familial adenomatous polyposis (FAP) suffering from duodenal adenomatosis. We investigated the in vitro effects on cell proliferation, apoptosis, and COX-2 expression of the potential chemopreventives celecoxib and tauro-ursodeoxycholic acid (UDCA). HT-29 colon cancer cells and LT97 colorectal micro-adenoma cells derived from a patient with FAP, were exposed to low dose celecoxib and UDCA alone or in combination with tauro-cholic acid (CA) and tauro-chenodeoxycholic acid (CDCA), mimicking bile of FAP patients treated with UDCA. In HT-29 cells, co-treatment with low dose celecoxib and UDCA resulted in a decreased cell growth (14-17%, p < 0.01). A more pronounced decrease (23-27%, p < 0.01) was observed in LT97 cells. Cell growth of HT-29 cells exposed to 'artificial bile' enriched with UDCA, was decreased (p < 0.001), either in the absence or presence of celecoxib. In LT97 cells incubated with 'artificial bile' enriched with UDCA, cell growth was decreased only in the presence of celecoxib (p < 0.05). No clear evidence was found for involvement of proliferating cell nuclear antigen, caspase-3, or COX-2 in the cellular processes leading to the observed changes in cell growth. In conclusion, co-treatment with low dose celecoxib and UDCA has growth inhibitory effects on colorectal adenoma cells derived from a patient with FAP, and further research on this combination as promising chemopreventive strategy is desired. -- Highlights: Black-Right-Pointing-Pointer Celecoxib and UDCA acid co-treatment decreases cell growth in colon tumor cells. Black-Right-Pointing-Pointer UDCA enriched 'artificial bile' decreases LT-97 cell growth only in presence of celecoxib. Black-Right-Pointing-Pointer PCNA, caspase-3, nor COX-2 seem to be involved in the observed changes in cell growth.

Celecoxib and tauro-ursodeoxycholic acid co-treatment inhibits cell growth in familial adenomatous polyposis derived LT97 colon adenoma cells

International Nuclear Information System (INIS)

Heumen, Bjorn W.H. van; Roelofs, Hennie M.J.; Morsche, René H.M. te; Marian, Brigitte; Nagengast, Fokko M.; Peters, Wilbert H.M.

2012-01-01

Chemoprevention would be a desirable strategy to avoid duodenectomy in patients with familial adenomatous polyposis (FAP) suffering from duodenal adenomatosis. We investigated the in vitro effects on cell proliferation, apoptosis, and COX-2 expression of the potential chemopreventives celecoxib and tauro-ursodeoxycholic acid (UDCA). HT-29 colon cancer cells and LT97 colorectal micro-adenoma cells derived from a patient with FAP, were exposed to low dose celecoxib and UDCA alone or in combination with tauro-cholic acid (CA) and tauro-chenodeoxycholic acid (CDCA), mimicking bile of FAP patients treated with UDCA. In HT-29 cells, co-treatment with low dose celecoxib and UDCA resulted in a decreased cell growth (14–17%, p < 0.01). A more pronounced decrease (23–27%, p < 0.01) was observed in LT97 cells. Cell growth of HT-29 cells exposed to ‘artificial bile’ enriched with UDCA, was decreased (p < 0.001), either in the absence or presence of celecoxib. In LT97 cells incubated with ‘artificial bile’ enriched with UDCA, cell growth was decreased only in the presence of celecoxib (p < 0.05). No clear evidence was found for involvement of proliferating cell nuclear antigen, caspase-3, or COX-2 in the cellular processes leading to the observed changes in cell growth. In conclusion, co-treatment with low dose celecoxib and UDCA has growth inhibitory effects on colorectal adenoma cells derived from a patient with FAP, and further research on this combination as promising chemopreventive strategy is desired. -- Highlights: ► Celecoxib and UDCA acid co-treatment decreases cell growth in colon tumor cells. ► UDCA enriched ‘artificial bile’ decreases LT-97 cell growth only in presence of celecoxib. ► PCNA, caspase-3, nor COX-2 seem to be involved in the observed changes in cell growth.

SIRT1 deacetylates RFX5 and antagonizes repression of collagen type I (COL1A2) transcription in smooth muscle cells

Energy Technology Data Exchange (ETDEWEB)

Xia, Jun [Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University (China); Department of Respiratory Medicine, Jiangsu Provincial Hospital of Chinese Traditional Medicine (China); Wu, Xiaoyan; Yang, Yuyu; Zhao, Yuhao [Atherosclerosis Research Center, Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Pathophysiology, Nanjing Medical University (China); Fang, Mingming [Jiangsu Jiankang Vocational Institute (China); Xie, Weiping, E-mail: wpxienjmu@gmail.com [Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University (China); Wang, Hong, E-mail: hwangnjmu@gmail.com [Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University (China); Xu, Yong [Atherosclerosis Research Center, Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Pathophysiology, Nanjing Medical University (China)

2012-11-16

Highlights: Black-Right-Pointing-Pointer SIRT1 interacts with and deacetylates RFX5. Black-Right-Pointing-Pointer SIRT1 activation attenuates whereas SIRT1 inhibition enhances collagen repression by RFX5 in vascular smooth muscle cells. Black-Right-Pointing-Pointer SIRT1 promotes cytoplasmic localization and proteasomal degradation of RFX5 and cripples promoter recruitment of RFX5. Black-Right-Pointing-Pointer IFN-{gamma} represses SIRT1 expression in vascular smooth muscle cells. Black-Right-Pointing-Pointer SIRT1 agonist alleviates collagen repression by IFN-{gamma} in vascular smooth muscle cells. -- Abstract: Decreased expression of collagen by vascular smooth muscle cells (SMCs) within the atherosclerotic plaque contributes to the thinning of the fibrous cap and poses a great threat to plaque rupture. Elucidation of the mechanism underlying repressed collagen type I (COL1A2) gene would potentially provide novel solutions that can prevent rupture-induced complications. We have previously shown that regulatory factor for X-box (RFX5) binds to the COL1A2 transcription start site and represses its transcription. Here we report that SIRT1, an NAD-dependent, class III deacetylase, forms a complex with RFX5. Over-expression of SIRT1 or NAMPT, which synthesizes NAD+ to activate SIRT1, or treatment with the SIRT1 agonist resveratrol decreases RFX5 acetylation and disrupts repression of the COL1A2 promoter activity by RFX5. On the contrary, knockdown of SIRT1 or treatment with SIRT1 inhibitors induces RFX5 acetylation and enhances the repression of collagen transcription. SIRT1 antagonizes RFX5 activity by promoting its nuclear expulsion and proteasomal degradation hence dampening its binding to the COL1A2 promoter. The pro-inflammatory cytokine IFN-{gamma} represses COL1A2 transcription by down-regulating SIRT1 expression in SMCs. Therefore, our data have identified as novel pathway whereby SIRT1 maintains collagen synthesis in SMCs by modulating RFX5 activity.

The different requirement of L-T4 therapy in congenital athyreosis compared with adult-acquired hypothyroidism suggests a persisting thyroid hormone resistance at the hypothalamic-pituitary level.

Science.gov (United States)

Bagattini, Brunella; Cosmo, Caterina Di; Montanelli, Lucia; Piaggi, Paolo; Ciampi, Mariella; Agretti, Patrizia; Marco, Giuseppina De; Vitti, Paolo; Tonacchera, Massimo

2014-11-01

Levothyroxine (l-T4) is commonly employed to correct hormone deficiency in children with congenital hypothyroidism (CH) and in adult patients with iatrogenic hypothyroidism. To compare the daily weight-based dosage of the replacement therapy with l-T4 in athyreotic adult patients affected by CH and adult patients with thyroid nodular or cancer diseases treated by total thyroidectomy. A total of 36 adult patients (27 females and nine males) aged 18-29 years were studied; 13 patients (age: 21.5±2.1, group CH) had athyreotic CH treated with l-T4 since the first days of life. The remaining 23 patients (age: 24±2.7, group AH) had hypothyroidism after total thyroidectomy (14 patients previously affected by nodular disease and nine by thyroid carcinoma with clinical and biochemical remission). Patient weight, serum free thyroid hormones, TSH, thyroglobulin (Tg), anti-Tg, and anti-thyroperoxidase antibodies were measured. Required l-T4 dosage was evaluated. At the time of the observations, all patients presented free thyroid hormones within the normal range and TSH between 0.8 and 2 μIU/ml. Patients had undetectable Tg and anti-thyroid antibodies. The daily weight-based dosage of the replacement therapy with l-T4 to reach euthyroidism in patients of group CH was significantly higher than that in those of group AH (2.16±0.36 vs 1.73±0.24 μg/kg, Phypothyroidism, patients of group CH required a daily l-T4 dose/kg higher than group AH patients, despite higher levels of TSH. The different requirement of replacement therapy between adult patients with congenital and those with surgical athyroidism could be explained by a lack of thyroid hormones since fetal life in CH, which could determine a different set point of the hypothalamus-pituitary-thyroid axis. © 2014 European Society of Endocrinology.

SWAAM-LT: The long-term, sodium/water reaction analysis method computer code

International Nuclear Information System (INIS)

Shin, Y.W.; Chung, H.H.; Wiedermann, A.H.; Tanabe, H.

1993-01-01

The SWAAM-LT Code, developed for analysis of long-term effects of sodium/water reactions, is discussed. The theoretical formulation of the code is described, including the introduction of system matrices for ease of computer programming as a general system code. Also, some typical results of the code predictions for available large scale tests are presented. Test data for the steam generator design with the cover-gas feature and without the cover-gas feature are available and analyzed. The capabilities and limitations of the code are then discussed in light of the comparison between the code prediction and the test data

Thunderbolts and Eggshells: Composite Air Operations During Desert Storm and Implications for USAF Doctrine and Force Structure

Science.gov (United States)

1994-09-01

2-5. 60. See UT Col Mark A. Welsh, "lay ot the Killer Scouts." Air Porne Magazine, April 1993, 66-70. 61. T’imothy T’. Lupfer, "The D)ynamics of D...TFW - Tactical Fighter Wing TFW(P) - Tactical Fighter Wing (Provisional) TLFE - Theater Large Force Employment Exercise UAE - United Arab Emirates

CNV analysis in Tourette syndrome implicates large genomic rearrangements in COL8A1 and NRXN1.

Directory of Open Access Journals (Sweden)

Abhishek Nag

Full Text Available Tourette syndrome (TS is a neuropsychiatric disorder with a strong genetic component. However, the genetic architecture of TS remains uncertain. Copy number variation (CNV has been shown to contribute to the genetic make-up of several neurodevelopmental conditions, including schizophrenia and autism. Here we describe CNV calls using SNP chip genotype data from an initial sample of 210 TS cases and 285 controls ascertained in two Latin American populations. After extensive quality control, we found that cases (N = 179 have a significant excess (P = 0.006 of large CNV (>500 kb calls compared to controls (N = 234. Amongst 24 large CNVs seen only in the cases, we observed four duplications of the COL8A1 gene region. We also found two cases with ∼400 kb deletions involving NRXN1, a gene previously implicated in neurodevelopmental disorders, including TS. Follow-up using multiplex ligation-dependent probe amplification (and including 53 more TS cases validated the CNV calls and identified additional patients with rearrangements in COL8A1 and NRXN1, but none in controls. Examination of available parents indicates that two out of three NRXN1 deletions detected in the TS cases are de-novo mutations. Our results are consistent with the proposal that rare CNVs play a role in TS aetiology and suggest a possible role for rearrangements in the COL8A1 and NRXN1 gene regions.

CNV analysis in Tourette syndrome implicates large genomic rearrangements in COL8A1 and NRXN1.

Science.gov (United States)

Nag, Abhishek; Bochukova, Elena G; Kremeyer, Barbara; Campbell, Desmond D; Muller, Heike; Valencia-Duarte, Ana V; Cardona, Julio; Rivas, Isabel C; Mesa, Sandra C; Cuartas, Mauricio; Garcia, Jharley; Bedoya, Gabriel; Cornejo, William; Herrera, Luis D; Romero, Roxana; Fournier, Eduardo; Reus, Victor I; Lowe, Thomas L; Farooqi, I Sadaf; Mathews, Carol A; McGrath, Lauren M; Yu, Dongmei; Cook, Ed; Wang, Kai; Scharf, Jeremiah M; Pauls, David L; Freimer, Nelson B; Plagnol, Vincent; Ruiz-Linares, Andrés

2013-01-01

Tourette syndrome (TS) is a neuropsychiatric disorder with a strong genetic component. However, the genetic architecture of TS remains uncertain. Copy number variation (CNV) has been shown to contribute to the genetic make-up of several neurodevelopmental conditions, including schizophrenia and autism. Here we describe CNV calls using SNP chip genotype data from an initial sample of 210 TS cases and 285 controls ascertained in two Latin American populations. After extensive quality control, we found that cases (N = 179) have a significant excess (P = 0.006) of large CNV (>500 kb) calls compared to controls (N = 234). Amongst 24 large CNVs seen only in the cases, we observed four duplications of the COL8A1 gene region. We also found two cases with ∼400 kb deletions involving NRXN1, a gene previously implicated in neurodevelopmental disorders, including TS. Follow-up using multiplex ligation-dependent probe amplification (and including 53 more TS cases) validated the CNV calls and identified additional patients with rearrangements in COL8A1 and NRXN1, but none in controls. Examination of available parents indicates that two out of three NRXN1 deletions detected in the TS cases are de-novo mutations. Our results are consistent with the proposal that rare CNVs play a role in TS aetiology and suggest a possible role for rearrangements in the COL8A1 and NRXN1 gene regions.

Common variants in the COL4A4 gene confer susceptibility to lattice degeneration of the retina.

Science.gov (United States)

Meguro, Akira; Ideta, Hidenao; Ota, Masao; Ito, Norihiko; Ideta, Ryuichi; Yonemoto, Junichi; Takeuchi, Masaki; Uemoto, Riyo; Nishide, Tadayuki; Iijima, Yasuhito; Kawagoe, Tatsukata; Okada, Eiichi; Shiota, Tomoko; Hagihara, Yuta; Oka, Akira; Inoko, Hidetoshi; Mizuki, Nobuhisa

2012-01-01

Lattice degeneration of the retina is a vitreoretinal disorder characterized by a visible fundus lesion predisposing the patient to retinal tears and detachment. The etiology of this degeneration is still uncertain, but it is likely that both genetic and environmental factors play important roles in its development. To identify genetic susceptibility regions for lattice degeneration of the retina, we performed a genome-wide association study (GWAS) using a dense panel of 23,465 microsatellite markers covering the entire human genome. This GWAS in a Japanese cohort (294 patients with lattice degeneration and 294 controls) led to the identification of one microsatellite locus, D2S0276i, in the collagen type IV alpha 4 (COL4A4) gene on chromosome 2q36.3. To validate the significance of this observation, we evaluated the D2S0276i region in the GWAS cohort and in an independent Japanese cohort (280 patients and 314 controls) using D2S0276i and 47 single nucleotide polymorphisms covering the region. The strong associations were observed in D2S0276i and rs7558081 in the COL4A4 gene (Pc = 5.8 × 10(-6), OR = 0.63 and Pc = 1.0 × 10(-5), OR = 0.69 in a total of 574 patients and 608 controls, respectively). Our findings suggest that variants in the COL4A4 gene may contribute to the development of lattice degeneration of the retina.

Common variants in the COL4A4 gene confer susceptibility to lattice degeneration of the retina.

Directory of Open Access Journals (Sweden)

Akira Meguro

Full Text Available Lattice degeneration of the retina is a vitreoretinal disorder characterized by a visible fundus lesion predisposing the patient to retinal tears and detachment. The etiology of this degeneration is still uncertain, but it is likely that both genetic and environmental factors play important roles in its development. To identify genetic susceptibility regions for lattice degeneration of the retina, we performed a genome-wide association study (GWAS using a dense panel of 23,465 microsatellite markers covering the entire human genome. This GWAS in a Japanese cohort (294 patients with lattice degeneration and 294 controls led to the identification of one microsatellite locus, D2S0276i, in the collagen type IV alpha 4 (COL4A4 gene on chromosome 2q36.3. To validate the significance of this observation, we evaluated the D2S0276i region in the GWAS cohort and in an independent Japanese cohort (280 patients and 314 controls using D2S0276i and 47 single nucleotide polymorphisms covering the region. The strong associations were observed in D2S0276i and rs7558081 in the COL4A4 gene (Pc = 5.8 × 10(-6, OR = 0.63 and Pc = 1.0 × 10(-5, OR = 0.69 in a total of 574 patients and 608 controls, respectively. Our findings suggest that variants in the COL4A4 gene may contribute to the development of lattice degeneration of the retina.

Effekten af hydraulisk kontrol på den vertikale fordeling og sammensætning af fytoplankton og zooplanktonsamfundene i Lillebælt

DEFF Research Database (Denmark)

Nielsen, Erik Haar; Christensen, Jens Tang; Lund-Hansen, Lars Chresten

Effekten af strømbetinget opblanding på fytoplankton og zooplankton sammensætningen i en lagdelt vandsøjle blev undersøgt i Lillebælt i april 2015. De hydrografiske forhold blev kortlagt gennem kontinuerlige målinger med ADCP og undulerende CTD (Scanfish III) langs et transekt fra Tragten nord...... for Fredericia til det centrale Lillebælt. Vandprøver til analyse af fytoplankton blev taget fra tre strata med CTD forsynet med en rosette med Niskin -vandhentere og zooplankton blev indsamlet med et Multinet-maxi (HydroBios, Kiel) fra 9 strata i vandsøjlen. Under kraftig sydgående strøm viste hydrografien...

Maintainer’s Associate Training Instructional Environment: Its Demonstration (MATIE 2)

Science.gov (United States)

1989-06-01

Force Human Resources Laboratory, Brooks AFB, Texas for their support. We would especially like to thank Capt Kevin Kline, Lt Col Hugh Bums, and Mr...impossible. Thus, technicians need to be able to augment general understanding through use of technical documentation when automiated diagnostic systems...34 which incorporate human -like knowledge of three types: procedural, declarative, and qualitative. Procedural knowledge is that which Is required to

Defeating ISIS by Winning the War of Ideas

Science.gov (United States)

2017-03-17

Partial Fulfillment of the Graduation Requirements Advisor: Lt Col Brande Walton Maxwell Air Force Base, Alabama 17 March 2017 DISTRIBUTION A...with their lives due to economic or emotional struggle could fall victim to ISIS messaging. ISIS messaging is sophisticated, attracting several...slavery, denying women’s rights, rape, torture, and jihadi groups are all forbidden.53 Though many Muslims have been branded as “radical extremists

Lessons Learned from A System-Wide Evidence-Based Practice Program Implementation

Science.gov (United States)

2017-04-25

incorporating scientific evidence, clinical expertise and the patient’s values and preferences to provide quality healthcare . Despite growing...MEMORANDUM FOR ST DEPARTMENT OF THE AIR FORCE 59TH MEDICAL WING (AETC) JOINT BASE SAN ANTONIO - LACKLAND TEXAS ATTN: LT COL JACQUELINE KILLIAN...FROM: 59 MDW/SGVU SUBJECT: Professional Presentation Approval 14 FEB 2017 1. Your paper, entitled Lesson Learned From A System-Wide Evidence- Based

Defense or Diplomacy Geographic Combatant Commands

Science.gov (United States)

2011-06-01

originally ruled by 1 Priest, Dana , The Mission: Waging War and Keeping Peace with America’s Military...events. US Central Command. Dana Priest describes General Zinni‟s experience as a GCC commander, wherein General Zinni found that in many ways...ignored altogether. Dr. James Forsyth and Lt Col Chance Saltzman make this argument in their Air and Space Power Journal article “Stay Out —Why

Cisto de colédoco: experiência de cinco anos com o tratamento cirúrgico no hospital municipal JESUS-RJ

Directory of Open Access Journals (Sweden)

Lisieux Eyer de Jesus

Full Text Available OBJETIVO: Relatar a experiência no diagnóstico e tratamento do cisto de colédoco no Hospital Municipal Jesus em cinco anos (1996-2001 e a experiência brasileira publicada para o tratamento do cisto de colédoco em crianças. MÉTODO: Avaliação prospectiva dos pacientes operados com o diagnóstico de cisto de colédoco em cinco anos no Hospital Municipal Jesus. RESULTADOS: Houve predomínio de casos diagnosticados pela presença de dor abdominal recorrente, icterícia e/ou massa abdominal palpável. Apenas dois pacientes apresentavam a tríade completa. Todos os pacientes foram submetidos à ultra-sonografia e houve dúvida no diagnóstico ultra-sonográfico em apenas dois. Todos eram cistos do tipo 1 de Todani tratados através de ressecção completa e anastomose biliodigestiva em Y Roux. Houve complicações pós-operatórias imediatas em três pacientes: um caso de hemorragia digestiva alta e dois casos de fístula biliar, um deles relacionado a problemas técnicos na anastomose que necessitou de reoperação. O seguimento variou de quatro meses a cinco anos, sem detecção de complicação tardia em nenhum caso. CONCLUSÕES: O tratamento do cisto de colédoco através da ressecção completa na criança é seguro, relacionado a poucas complicações e capaz de resolver por completo a sintomatologia pré-operatória dos pacientes.

Role of CTA1R7K-COL-DD as a novel therapeutic mucosal tolerance-inducing vector for treatment of collagen-induced arthritis.

Science.gov (United States)

Hasselberg, Annemarie; Schön, Karin; Tarkowski, Andrej; Lycke, Nils

2009-06-01

To determine whether a cholera toxin-derived, novel immunomodulating fusion protein, CTA1R7K-COL-DD, carrying the class II major histocompatibility complex H-2q-restricted type II collagen peptide aa 259-274, can induce therapeutic tolerance and prevent collagen-induced arthritis (CIA) when administered intranasally in DBA/1 mice, and to assess whether ADP-ribosylation at the mucosal membranes exerts a regulatory function such that the outcome of tolerance or immune enhancement can be controlled. DBA/1 mice with CIA were treated intranasally with CTA1R7K-COL-DD. The therapeutic effect was monitored for 46 days after the onset of disease. Clinical scoring of disease, histologic examination of inflammation, and bone erosion were assessed, and cytokine levels were determined in the serum or supernatants from splenocytes stimulated with recall antigen. The protective effect of CTA1R7K-COL-DD resulted in roughly 60% of the mice having no clinical signs or histologic evidence of disease after treatment, and those with CIA had significantly milder disease with less bone erosion. The protective status was associated with lower serum titers of IgG1, IgG2a, IgG2b, and IgG3 anticollagen and a substantial decrease in the production of interleukin-6 (IL-6), IL-17, and interferon-gamma, while levels of IL-10 were markedly up-regulated both in the serum and at the T cell level. The enzymatically inactive mutant fusion protein CTA1R7K-COL-DD provided substantial therapeutic protection against CIA following intranasal administration. The mechanism behind the effect appears to be mediated by peptide-specific regulatory T cells induced by mucosal exposure to the peptide containing CTA1R7K-COL-DD vector. In addition, ADP-ribosylation at the mucosal membranes acts as a key regulator controlling mucosal tolerance or immunity.

Genetic and Epigenetic Factors at COL2A1 and ABCA4 Influence Clinical Outcome in Congenital Toxoplasmosis

Science.gov (United States)

Jamieson, Sarra E.; de Roubaix, Lee-Anne; Cortina-Borja, Mario; Tan, Hooi Kuan; Mui, Ernest J.; Cordell, Heather J.; Kirisits, Michael J.; Miller, E. Nancy; Peacock, Christopher S.; Hargrave, Aubrey C.; Coyne, Jessica J.; Boyer, Kenneth; Bessieres, Marie-Hélène; Buffolano, Wilma; Ferret, Nicole; Franck, Jacqueline; Kieffer, François; Meier, Paul; Nowakowska, Dorota E.; Paul, Malgorzata; Peyron, François; Stray-Pedersen, Babill; Prusa, Andrea-Romana; Thulliez, Philippe; Wallon, Martine; Petersen, Eskild; McLeod, Rima; Gilbert, Ruth E.; Blackwell, Jenefer M.

2008-01-01

Background Primary Toxoplasma gondii infection during pregnancy can be transmitted to the fetus. At birth, infected infants may have intracranial calcification, hydrocephalus, and retinochoroiditis, and new ocular lesions can occur at any age after birth. Not all children who acquire infection in utero develop these clinical signs of disease. Whilst severity of disease is influenced by trimester in which infection is acquired by the mother, other factors including genetic predisposition may contribute. Methods and Findings In 457 mother-child pairs from Europe, and 149 child/parent trios from North America, we show that ocular and brain disease in congenital toxoplasmosis associate with polymorphisms in ABCA4 encoding ATP-binding cassette transporter, subfamily A, member 4. Polymorphisms at COL2A1 encoding type II collagen associate only with ocular disease. Both loci showed unusual inheritance patterns for the disease allele when comparing outcomes in heterozygous affected children with outcomes in affected children of heterozygous mothers. Modeling suggested either an effect of mother's genotype, or parent-of-origin effects. Experimental studies showed that both ABCA4 and COL2A1 show isoform-specific epigenetic modifications consistent with imprinting. Conclusions These associations between clinical outcomes of congenital toxoplasmosis and polymorphisms at ABCA4 and COL2A1 provide novel insight into the molecular pathways that can be affected by congenital infection with this parasite. PMID:18523590

Genetic and epigenetic factors at COL2A1 and ABCA4 influence clinical outcome in congenital toxoplasmosis.

Directory of Open Access Journals (Sweden)

Sarra E Jamieson

2008-06-01

Full Text Available Primary Toxoplasma gondii infection during pregnancy can be transmitted to the fetus. At birth, infected infants may have intracranial calcification, hydrocephalus, and retinochoroiditis, and new ocular lesions can occur at any age after birth. Not all children who acquire infection in utero develop these clinical signs of disease. Whilst severity of disease is influenced by trimester in which infection is acquired by the mother, other factors including genetic predisposition may contribute.In 457 mother-child pairs from Europe, and 149 child/parent trios from North America, we show that ocular and brain disease in congenital toxoplasmosis associate with polymorphisms in ABCA4 encoding ATP-binding cassette transporter, subfamily A, member 4. Polymorphisms at COL2A1 encoding type II collagen associate only with ocular disease. Both loci showed unusual inheritance patterns for the disease allele when comparing outcomes in heterozygous affected children with outcomes in affected children of heterozygous mothers. Modeling suggested either an effect of mother's genotype, or parent-of-origin effects. Experimental studies showed that both ABCA4 and COL2A1 show isoform-specific epigenetic modifications consistent with imprinting.These associations between clinical outcomes of congenital toxoplasmosis and polymorphisms at ABCA4 and COL2A1 provide novel insight into the molecular pathways that can be affected by congenital infection with this parasite.

A SEARCH FOR L/T TRANSITION DWARFS WITH Pan-STARRS1 AND WISE: DISCOVERY OF SEVEN NEARBY OBJECTS INCLUDING TWO CANDIDATE SPECTROSCOPIC VARIABLES

International Nuclear Information System (INIS)

Best, William M. J.; Liu, Michael C.; Magnier, Eugene A.; Aller, Kimberly M.; Burgett, W. S.; Chambers, K. C.; Hodapp, K. W.; Kaiser, N.; Kudritzki, R.-P.; Morgan, J. S.; Tonry, J. L.; Wainscoat, R. J.; Deacon, Niall R.; Dupuy, Trent J.; Redstone, Joshua; Price, P. A.

2013-01-01

We present initial results from a wide-field (30,000 deg 2 ) search for L/T transition brown dwarfs within 25 pc using the Pan-STARRS1 and Wide-field Infrared Survey Explorer (WISE) surveys. Previous large-area searches have been incomplete for L/T transition dwarfs, because these objects are faint in optical bands and have near-infrared (near-IR) colors that are difficult to distinguish from background stars. To overcome these obstacles, we have cross-matched the Pan-STARRS1 (optical) and WISE (mid-IR) catalogs to produce a unique multi-wavelength database for finding ultracool dwarfs. As part of our initial discoveries, we have identified seven brown dwarfs in the L/T transition within 9-15 pc of the Sun. The L9.5 dwarf PSO J140.2308+45.6487 and the T1.5 dwarf PSO J307.6784+07.8263 (both independently discovered by Mace et al.) show possible spectroscopic variability at the Y and J bands. Two more objects in our sample show evidence of photometric J-band variability, and two others are candidate unresolved binaries based on their spectra. We expect our full search to yield a well-defined, volume-limited sample of L/T transition dwarfs that will include many new targets for study of this complex regime. PSO J307.6784+07.8263 in particular may be an excellent candidate for in-depth study of variability, given its brightness (J = 14.2 mag) and proximity (11 pc)

Tríplice fronteira Brasil, Peru e Colômbia e as implicações com o narcotráfico

OpenAIRE

Dias Balieiro, Luiz Felipe de Vasconcelos; Nascimento, Izaura Rodrigues

2015-01-01

Na região do Alto Solimões, extremo oeste do Amazonas, localiza-se a chamada Tríplice Fronteira Amazônica, formada pelas cidades de Tabatinga/Brasil, Santa Rosa/Peru e Letícia/Colômbia. Esta zona fronteiriça é identificada como uma das portas de entrada dos entorpecentes oriundos das zonas produtoras do Peru e Colômbia no território brasileiro. Este artigo objetiva promover a análise das características do narcotráfico na tríplice fronteira amazônica, com uma breve exposição do seu processo d...

Intranasal Administration of 2/6-Rotavirus-Like Particles with Mutant Escherichia coli Heat-Labile Toxin (LT-R192G) Induces Antibody-Secreting Cell Responses but Not Protective Immunity in Gnotobiotic Pigs

Science.gov (United States)

Yuan, Lijuan; Geyer, Annelise; Hodgins, Douglas C.; Fan, Zhiqian; Qian, Yuan; Chang, Kyeong-Ok; Crawford, Sue E.; Parreño, Viviana; Ward, Lucy A.; Estes, Mary K.; Conner, Margaret E.; Saif, Linda J.

2000-01-01

We investigated the immunogenicity of recombinant double-layered rotavirus-like particle (2/6-VLPs) vaccines derived from simian SA11 or human (VP6) Wa and bovine RF (VP2) rotavirus strains. The 2/6-VLPs were administered to gnotobiotic pigs intranasally (i.n.) with a mutant Escherichia coli heat-labile toxin, LT-R192G (mLT), as mucosal adjuvant. Pigs were challenged with virulent Wa (P1A[8],G1) human rotavirus at postinoculation day (PID) 21 (two-dose VLP regimen) or 28 (three-dose VLP regimen). In vivo antigen-activated antibody-secreting cells (ASC) (effector B cells) and in vitro antigen-reactivated ASC (derived from memory B cells) from intestinal and systemic lymphoid tissues (duodenum, ileum, mesenteric lymph nodes [MLN], spleen, peripheral blood lymphocytes [PBL], and bone marrow lymphocytes) collected at selected times were quantitated by enzyme-linked immunospot assays. Rotavirus-specific immunoglobulin M (IgM), IgA, and IgG ASC and memory B-cell responses were detected by PID 21 or 28 in intestinal and systemic lymphoid tissues after i.n. inoculation with two or three doses of 2/6-VLPs with or without mLT. Greater mean numbers of virus-specific ASC and memory B cells in all tissues prechallenge were induced in pigs inoculated with two doses of SA11 2/6-VLPs plus mLT compared to SA11 2/6-VLPs without mLT. After challenge, anamnestic IgA and IgG ASC and memory B-cell responses were detected in intestinal lymphoid tissues of all VLP-inoculated groups, but serum virus-neutralizing antibody titers were not significantly enhanced compared to the challenged controls. Pigs inoculated with Wa-RF 2/6-VLPs (with or without mLT) developed higher anamnestic IgA and IgG ASC responses in ileum after challenge compared to pigs inoculated with SA11 2/6-VLPs (with or without mLT). Three doses of SA 11 2/6-VLP plus mLT induced the highest mean numbers of IgG memory B cells in MLN, spleen, and PBL among all groups postchallenge. However, no significant protection against

Discovery of a visual T-dwarf triple system and binarity at the L/T transition

Energy Technology Data Exchange (ETDEWEB)

Radigan, Jacqueline [Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD 21218 (United States); Jayawardhana, Ray [Department of Astronomy and Astrophysics, University of Toronto, 50 St. George Street, Toronto, ON M5S 3H4 (Canada); Lafrenière, David [Département de Physique, Université de Montréal, C.P. 6128 Succ. Centre-Ville, Montréal, QC H3C 3J7 (Canada); Dupuy, Trent J. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Liu, Michael C. [Institute for Astronomy, University of Hawaii, 2680 Woodlawn Drive, Honolulu, HI 96822 (United States); Scholz, Alexander, E-mail: radigan@stsci.edu [School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, KY169SS (United Kingdom)

2013-11-20

We present new high contrast imaging of eight L/T transition brown dwarfs (BDs) using the NIRC2 camera on the Keck II telescope. One of our targets, the T3.5 dwarf 2MASS J08381155+1511155, was resolved into a hierarchal triple with projected separations of 2.5 ± 0.5 AU and 27 ± 5 AU for the BC and A(BC) components, respectively. Resolved OSIRIS spectroscopy of the A(BC) components confirms that all system members are T dwarfs. The system therefore constitutes the first triple T-dwarf system ever reported. Using resolved photometry to model the integrated-light spectrum, we infer spectral types of T3 ± 1, T3 ± 1, and T4.5 ± 1 for the A, B, and C components, respectively. The uniformly brighter primary has a bluer J – K{sub s} color than the next faintest component, which may reflect a sensitive dependence of the L/T transition temperature on gravity, or alternatively divergent cloud properties among components. Relying on empirical trends and evolutionary models we infer a total system mass of 0.034-0.104 M {sub ☉} for the BC components at ages of 0.3-3 Gyr, which would imply a period of 12-21 yr assuming the system semimajor axis to be similar to its projection. We also infer differences in effective temperatures and surface gravities between components of no more than ∼150 K and ∼0.1 dex. Given the similar physical properties of the components, the 2M0838+15 system provides a controlled sample for constraining the relative roles of effective temperature, surface gravity, and dust clouds in the poorly understood L/T transition regime. For an age of 3 Gyr we estimate a binding energy of ∼20 × 10{sup 41} erg for the wide A(BC) pair, which falls above the empirical minimum found for typical BD binaries, and suggests that the system may have been able to survive a dynamical ejection during formation. Combining our imaging survey results with previous work we find an observed binary fraction of 4/18 or 22{sub −8}{sup +10}% for unresolved spectral types

Participation des médecins généralistes de la province de Benimellal (Maroc) dans le dépistage du cancer du col

Science.gov (United States)

Nani, Samira; Benallal, Mohamed; Hassoune, Samira; Kissi, Dounia; Maaroufi, Abderrahmane

2013-01-01

Introduction Au Maroc, chaque année il y aurait environ 2000 nouveaux cas de cancer du col et les 2/3 des cas sont pris en charge à un stade très avancé. Nous avons mené une étude transversale, exhaustive incluant les 71 médecins généralistes exerçant dans les établissements de soins de santé de base du secteur public et privé de la province de Benimellal. Le but était d’évaluer leurs connaissances et leur participation au dépistage du cancer du col. Méthodes Nous avons mené une étude transversale, exhaustive incluant les 71 médecins généralistes exerçant dans les établissements de soins de santé de base du secteur public et privé de la province de Benimellal. Le but était d’évaluer leurs connaissances et leur participation au dépistage du cancer du col. Résultats Le niveau de connaissance était relativement modeste, 22 médecins généraliste avaient répondu à la question sur l'incidence du cancer du col au Maroc, Parmi eux (81,8%) avaient donné une réponse incorrecte. L'Herpes Papilloma virus comme facteur de risque du cancer du col a été identifié par seulement 21% des médecins généralistes. La participation au dépistage était également défaillante, 92,8% n'avaient jamais pratiqué le FCV chez leurs patientes à cause principalement du manque de formation (95,5%). Conclusion Les résultats montrent la nécessité d'améliorer les connaissances théoriques et pratique des médecins généralistes concernant le dépistage du cancer du col. PMID:23785557

Tempo de reepitelização corneana com a instilação de colírio contendo hialuronato de sódio e carboximetilcelulose

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Luciane Bugman Moreira

2013-10-01

Full Text Available OBJETIVO: Avaliar o tempo de reepitelização corneana pós abrasão usando colírios comercialmente disponíveis, um contendo hialuronato de sódio a 0,4%, outro contendo carboximetilcelulose a 1%, e comparar com a reepitelização sem instilação de colírio. MÉTODOS: Foram utilizados 24 coelhos, nos quais foi feita a abrasão mecânica da córnea nos 8 mm centrais. Esses animais foram divididos em três grupos. O primeiro grupo recebeu um colírio disponível comercialmente contendo hialuronato de sódio 0,4%, o segundo recebeu um colírio contendo carboximetilcelulose 1% e o terceiro não recebeu nenhuma droga. A avaliação foi feita a cada 24 horas por meio da análise de fotografias digitais sob luz azul de cobalto e coramento das córneas com fluoresceína a 2%. O estudo das imagens foi feito pelo sistema de análise de imagens do Autocad 2009®. A análise dos dados foi feita comparando o tempo total de reepitelização da córnea e a cada 24 horas entre os três grupos. RESULTADOS: A velocidade de reepitelização do grupo que usou colírio contendo hialuronato de sódio foi em média 90 horas; o grupo que usou carboximetilcelulose apresentou média de 105 horas; e o grupo que não usou nenhum tipo de lubrificante apresentou média de 108 horas para total reepitelização. Houve uma melhor performance na reepitelização após 96 horas nas córneas dos coelhos que usaram os colírios lubrificantes, sendo essa diferença estatisticamente comprovada. CONCLUSÃO: O colírio contendo hialuronato de sódio 0,4% mostrou índice de eficácia maior que aquele contendo carboximetilcelulose 1%, e este maior eficácia que o controle. Os resultados encontrados neste estudo mostram que o uso de lubrificantes no processo de reepitelização são de extrema valia e devem ser usados de rotina na clínica oftalmológica.

Colágeno asimilable. Fuente de prevención de enfermedades osteoarticuladas

OpenAIRE

Parra Parra, Yolanda; Quesada Martínez, María Inmaculada; Pérez Collado, Elisa María

2015-01-01

Cartel presentado en la Segunda Conferencia Internacional de Comunicación en Salud, celebrada el 23 de octubre de 2015 en la Universidad Carlos III de Madrid Introducción: El colágeno es la proteína más abundante de nuestro cuerpo humano y uno de sus componentes esencial de articulaciones, cartílago, ligamento, tendones, huesos, piel. Su especial estructura lo hacen único, presenta una estructura fibrosa, que aporta gran resistencia y flexibilidad a los tejidos de los que forma parte. Cu...

Distribución espacial de fibroblastos endoneurales en cultivos tridimensionales en geles de colágeno Tipo I

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Leslie Yaneth Leal Mejía

2002-07-01

Full Text Available SaludRESUMENEn la actualidad los cultivos celulares tridimensionales son muy utilizados, ya que imitan lascondiciones in vivode las células. Uno de los cultivos más desarrollados es el de fibroblastosdérmicos dentro de geles de colágeno en forma de lenteja, los cuales se contraen de un 70%a un 80% durante las primeras 24 horas de cultivo. El objetivo inicial de este trabajo fue aislarfibroblastos endoneurales (FE del nervio ciático de ratones ICR, mantenerlos en cultivo enmonocapa y luego en cultivo tridimensional en geles cilíndricos de colágeno tipo I de 1,6 mmde diámetro y 3 mm de largo, formados en las prótesis de silicona empleadas para regeneraciónde nervios periféricos; imitando las condiciones del nervio in vivopara que pueda ser emplea-dos en regeneración. Inicialmente se retira epineuro y perineuro del nervio, dejando sólo elendoneuro que se disocia con colagenasa (500U/ml y luego se le hace una disociación me-cánica. Las células libres, se cultivan en monocapa con DMEM suplementado con 20% suerobovino fetal, logrando un 95% de pureza en cultivo primario; en los subcultivos se purifican yamplifican los FE. Estos se aislan y se mezclan con una solución de colágeno tipo I a 0,5mg/mlpara la formación de los geles, teniendo en cuenta que cada gel contenga 50.000 fibroblastos,se introduce la mezcla dentro de las prótesis de silicona y se dejan gelificar, siguiendo el mismoprocedimiento se formaron geles sin células; una vez formados los geles se liberan de las cáma-ras y se cultivan en suspensión 1, 3, 5, 7, 15 y 30 días. Al cumplirse cada uno de los tiemposde cultivo, se analizó la apariencia del colágeno y la distribución de los FE en microscopíaóptica y de fluorescencia. Los cultivos primarios de FE se obtuvieron a los 5 días, lograndoacortar el tiempo de confluencia entre 15 y 20 días con respecto a lo reportado en la literatura;un dato novedoso fue la purificación total de los FE en el primer subcultivo

Association of COL1A1 rs1800012 polymorphism with musculoskeletal degenerative diseases: a meta-analysis.

Science.gov (United States)

Zhong, Binlong; Huang, Donghua; Ma, Kaige; Deng, Xiangyu; Shi, Deyao; Wu, Fashuai; Shao, Zengwu

2017-09-26

It has been reported that the single nucleotide polymorphism (SNP) rs1800012 in COL1A1 gene might be linked to the susceptibility of musculoskeletal degenerative diseases, such as osteoarthritis (OA) and intervertebral disc degeneration (IVDD). However, the data from different studies is contradictory. Here we aimed to comprehensively summarize and clarify the relationship between the SNP and musculoskeletal degenerative diseases. Seven eligible studies including 1339 cases and 5406 controls were screened out from PubMed, Web Of Science and Cochrane library databases. Significant association was identified in sub group analysis of IVDD in homozygote model (GG versus TT: OR = 0.33, 95% CI 0.14-0.78, P = 0.012), heterozygote model (GT versus TT: OR = 0.29, 95% CI 0.11-0.72, P = 0.008) and dominant model (GG/GT versus TT: OR = 0.31, 95% CI 0.13-0.74, P = 0.008). Additionally, significant relationship was also found in sub group analysis of severe degree of IVDD in homozygote model (GG versus TT: OR = 0.37, 95% CI 0.15-0.91, P = 0.031), heterozygote model (GT versus TT: OR = 0.33, 95% CI 0.13-0.87, P = 0.024) and dominant model (GG/GT versus TT: OR = 0.36, 95% CI 0.14-0.88, P = 0.025). Although no significance was observed, there is a trend that the more G allele at COL1A1 rs1800012 site, the less possibility of IVDD and severe IVDD would happen. Our results indicate that COL1A1 rs1800012 polymorphism associates with the susceptibility of IVDD. However, this polymorphism may not be associated with OA risk.

An Annotated Bibliography of Hypobaric Decompression Sickness Research Conducted at the Crew Technology Division, USAF School of Aerospace Medicine, Brooks AFB, Texas from 1983 to 1988

Science.gov (United States)

1990-06-01

AN ANNOTATED BIBLIOGRAPHY OF HYPOBARIC DECOMPRESSION SICKNESS RESEARCH CONDUCTED AT THE CREW TECHNOLOGY DIVISION, USAF SCHOOL OF AEROSPACE MEDICINE...190 man-flights to four selected altitudes (30000, 27500, 25000, and 22500 ft pressure equivalent) in a hypobaric chamber. The subjects’ ages ranged...conditions and two of these developed delayed sy~rtcms. Three of these five subjects underwent hyperbaric oxygen treatment. Conclusion. Female subjects

A SEARCH FOR L/T TRANSITION DWARFS WITH Pan-STARRS1 AND WISE: DISCOVERY OF SEVEN NEARBY OBJECTS INCLUDING TWO CANDIDATE SPECTROSCOPIC VARIABLES

Energy Technology Data Exchange (ETDEWEB)

Best, William M. J.; Liu, Michael C.; Magnier, Eugene A.; Aller, Kimberly M.; Burgett, W. S.; Chambers, K. C.; Hodapp, K. W.; Kaiser, N.; Kudritzki, R.-P.; Morgan, J. S.; Tonry, J. L.; Wainscoat, R. J. [Institute for Astronomy, University of Hawaii at Manoa, Honolulu, HI 96822 (United States); Deacon, Niall R. [Max Planck Institute for Astronomy, Koenigstuhl 17, D-69117 Heidelberg (Germany); Dupuy, Trent J. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Redstone, Joshua [Facebook, 335 Madison Ave, New York, NY 10017-4677 (United States); Price, P. A., E-mail: wbest@ifa.hawaii.edu [Department of Astrophysical Sciences, Princeton University, Princeton, NJ 08544 (United States)

2013-11-10

We present initial results from a wide-field (30,000 deg{sup 2}) search for L/T transition brown dwarfs within 25 pc using the Pan-STARRS1 and Wide-field Infrared Survey Explorer (WISE) surveys. Previous large-area searches have been incomplete for L/T transition dwarfs, because these objects are faint in optical bands and have near-infrared (near-IR) colors that are difficult to distinguish from background stars. To overcome these obstacles, we have cross-matched the Pan-STARRS1 (optical) and WISE (mid-IR) catalogs to produce a unique multi-wavelength database for finding ultracool dwarfs. As part of our initial discoveries, we have identified seven brown dwarfs in the L/T transition within 9-15 pc of the Sun. The L9.5 dwarf PSO J140.2308+45.6487 and the T1.5 dwarf PSO J307.6784+07.8263 (both independently discovered by Mace et al.) show possible spectroscopic variability at the Y and J bands. Two more objects in our sample show evidence of photometric J-band variability, and two others are candidate unresolved binaries based on their spectra. We expect our full search to yield a well-defined, volume-limited sample of L/T transition dwarfs that will include many new targets for study of this complex regime. PSO J307.6784+07.8263 in particular may be an excellent candidate for in-depth study of variability, given its brightness (J = 14.2 mag) and proximity (11 pc)

Toxicity and immunogenicity of Enterotoxigenic Escherichia coli heat-labile and heat-stable toxoid fusion 3xSTa(A14Q-LT(S63K/R192G/L211A in a murine model.

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Chengxian Zhang

Full Text Available Diarrhea is the second leading cause of death to young children. Enterotoxigenic Escherichia coli (ETEC are the most common bacteria causing diarrhea. Adhesins and enterotoxins are the virulence determinants in ETEC diarrhea. Adhesins mediate bacterial attachment and colonization, and enterotoxins including heat-labile (LT and heat-stable type Ib toxin (STa disrupt fluid homeostasis in host cells that leads to fluid hyper-secretion and diarrhea. Thus, adhesins and enterotoxins have been primarily targeted in ETEC vaccine development. A recent study reported toxoid fusions with STa toxoid (STa(P13F fused at the N- or C-terminus, or inside the A subunit of LT(R192G elicited neutralizing antitoxin antibodies, and suggested application of toxoid fusions in ETEC vaccine development (Liu et al., Infect. Immun. 79:4002-4009, 2011. In this study, we generated a different STa toxoid (STa(A14Q and a triple-mutant LT toxoid (LT(S63K/R192G/L211A, tmLT, constructed a toxoid fusion (3xSTa(A14Q-tmLT that carried 3 copies of STa(A14Q for further facilitation of anti-STa immunogenicity, and assessed antigen safety and immunogenicity in a murine model to explore its potential for ETEC vaccine development. Mice immunized with this fusion antigen showed no adverse effects, and developed antitoxin antibodies particularly through the IP route. Anti-LT antibodies were detected and were shown neutralizing against CT in vitro. Anti-STa antibodies were also detected in the immunized mice, and serum from the IP immunized mice neutralized STa toxin in vitro. Data from this study indicated that toxoid fusion 3xSTa(A14Q-tmLT is safe and can induce neutralizing antitoxin antibodies, and provided helpful information for vaccine development against ETEC diarrhea.

COL1A2 gene analysis in a Czech osteogenesis imperfecta patient: a candidate novel mutation in a patient affected by osteogenesis imperfecta type 3

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Hrušková L

2015-08-01

Full Text Available Lucie Hrušková,1 Ivo Mařík,2,3 Stella Mazurová,1 Pavel Martásek,1 Ivan Mazura1 1Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic; 2Ambulant Centre for Defects of Locomotor Apparatus 1.1.c., Prague, Czech Republic; 3Faculty of Medical Studies, West Bohemia University, Pilsen, Czech RepublicAbstract: Osteogenesis imperfecta is a heritable bone fragility disease with a heterogenic genetic origin. Most cases result from mutations of either the COL1A1 gene or the COL1A2 gene. We identified a novel COL1A2 gene mutation in a Czech patient, born to unaffected parents, who was diagnosed according to clinical and anthropometric findings and radiographic features as having type 3 osteogenesis imperfecta, which is a severe form of this disease. The identified Gly814Trp mutation was predicted by a number of complementary bioinformatic programs to result in functional alteration of the protein. This case report provides both evidence of a novel COL1A2 mutation resulting in type 3 osteogenesis imperfecta and a genotype:phenotype correlation in this affected individual. Keywords: osteogenesis imperfecta type 3, collagen, alpha-2 (I chain, substitution, sequencing 

CONTEMPT-LT/028: a computer program for predicting containment pressure-temperature response to a loss-of-coolant accident

International Nuclear Information System (INIS)

Hargroves, D.W.; Metcalfe, L.J.; Wheat, L.L.; Niederauer, G.F.; Obenchain, C.F.

1979-03-01

CONTEMPT-LT is a digital computer program, written in FORTRAN IV, developed to describe the long-term behavior of water-cooled nuclear reactor containment systems subjected to postulated loss-of-coolant accident (LOCA) conditions. The program calculates the time variation of compartment pressures, temperatures, mass and energy inventories, heat structure temperature distributions, and energy exchange with adjacent compartments. The program is capable of describing the effects of leakage on containment response. Models are provided to describe fan cooler and cooling spray engineered safety systems. An annular fan model is also provided to model pressure control in the annular region of dual containment systems. Up to four compartments can be modeled with CONTEMPT-LT, and any compartment except the reactor system may have both a liquid pool region and an air--vapor atmosphere region above the pool. Each region is assumed to have a uniform temperature, but the temperatures of the two regions may be different

Starter Kültür Kullanılarak Yapılan Tulum Peynirlerinin Bazı Özellikleri

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Zübeyde Öner

2015-02-01

Full Text Available Bu çalışmada, tulum peyniri kalitesi üzerinde starter kültür kullanımının etkileri ve tulum peyniri üretimine uygun starter kültür karışımı araştırılmıştır. Bu amaçla çiğ sütten ve starter kültür olarak Lactobacillus plantarum 1, Lb. plantarum 48, Lactococcus lactis 57, Enterococcus faecalis 40’ın farklı oranlardaki karışımları kullanılarak 3 farklı peynir üretilmiştir. 90 günlük olgunlaşma sonunda toplam kurumadde %57,85-61,09, SH 79,5-94 olgunlaşma katsayısı 51,48-136,32, kurumaddede tuz %4,03-5,26 kurumaddede yağ %54,42-57,29, toplam azot % 2,67-3,43 suda çözünen azot % 1,73-3,64 duyusal değerlendirme sonucu 52-72,81 toplam puan arasında değişmiştir. Mikrobiyolojik analiz sonuçlarına göre 90 günlük olgunlaşma sonunda toplam mezofil aerob bakteri sayısı 7,25-7,97 log kob/g, laktobasil 7,32-7,5 log kob/g, laktokok 7,71-8,15 log kob/g, koliform 1,73-5,02 log kob/g, S. aureus <10-2,35 log kob/g, enterokok 6,71-7,38 log kob/g, psikrofilik mikroorganizma <10 log kob/g, maya ve küf 4,22-5,49 log kob/g arasında değişmiştir.

Whole exome analysis identifies dominant COL4A1 mutations in patients with complex ocular phenotypes involving microphthalmia.

Science.gov (United States)

Deml, B; Reis, L M; Maheshwari, M; Griffis, C; Bick, D; Semina, E V

2014-11-01

Anophthalmia/microphthalmia (A/M) is a developmental ocular malformation defined as complete absence or reduction in size of the eye. A/M is a heterogenous disorder with numerous causative genes identified; however, about half the cases lack a molecular diagnosis. We undertook whole exome sequencing in an A/M family with two affected siblings, two unaffected siblings, and unaffected parents; the ocular phenotype was isolated with only mild developmental delay/learning difficulties reported and a normal brain magnetic resonance imaging (MRI) in the proband at 16 months. No pathogenic mutations were identified in 71 known A/M genes. Further analysis identified a shared heterozygous mutation in COL4A1, c.2317G>A, p.(Gly773Arg) that was not seen in the unaffected parents and siblings. Analysis of 24 unrelated A/M exomes identified a novel c.2122G>A, p.(Gly708Arg) mutation in an additional patient with unilateral microphthalmia, bilateral microcornea and Peters anomaly; the mutation was absent in the unaffected mother and the unaffected father was not available. Mutations in COL4A1 have been linked to a spectrum of human disorders; the most consistent feature is cerebrovascular disease with variable ocular anomalies, kidney and muscle defects. This study expands the spectrum of COL4A1 phenotypes and indicates screening in patients with A/M regardless of MRI findings or presumed inheritance pattern. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

The effect of triiodothyronine (L-T3) and weight loss on sex-hormone binding globulin (SHBG) in hirsute obese women

International Nuclear Information System (INIS)

Cavaliere, H.; Medeiros Neto, G.A.

1984-01-01

The effect of a 60-day period of treatment with L-T 3 (200 μg/day) was evaluated in hirsute obese women and normal controls. The obese patients were also submitted to a low calorie high protein. Mean initial weight significantly declined with a significant lowering of the mean DHEA-S leves. There was changes in serum T 3 rose in both obese and normal patients. Serum sex-hormone binding globulin levels at baseline studies were significantly higher in normal controls than in obese patients. At the end of de 60-day period of L-T 3 administration it was observed in both groups a 2-4 fold increase in serum SHBG levels but normal controls had significantly higher mean values than obese individuals. (M.A.C.) [pt

Confederate Staff Work At Chickamauga: An Analysis of the Staff of the Army of Tennessee.

Science.gov (United States)

1992-06-05

Strategy: from Machiavelli to the Nuclear Age, Peter Paret, ed., (Princeton: Princeton University Press, 1986), 143-185, presents an excellent summary...Lieut. P. B. Spence Asst. Inspector General Lieut. John Rawle Acting Chief of Ordnance Capt Felix H. Robertson Acting Chief of Artillery Maj. J. J...F. Sevier Asst. Inspector General Lieut. P. B. Spence Asst. Inspector General Lieut. John Rawle Acting Chief of Ordnance Lt. Col. Marshall T. Polk

Translations on Narcotics and Dangerous Drugs, Number 282.

Science.gov (United States)

1977-01-27

Judicial Police agents Francisco Fernandez Marin and Jesus Godoy Garcia. The arrest of Humberto de los Angeles Gutierrez and dentist Mario Alberto ...National Defense. He replaces Lt Col Alberto Alonso Rodriguez who has been named deputy commander of the 52nd Infantry Battalion in Chihuahua City...to sell in that city. Gaico Veremo Jacama (grower) 42 years of age, rural worker, living in Chibabava. Joao Mahala (smoker) bachelor, 34 years old

Internal Medicine in World War 2. Volume 1. Activities of Medical Consultants

Science.gov (United States)

1961-01-01

hiit ,v at t he 7t hi ienieral I los-pital to coniduct st (Idies tIer 1ili4he!. 1hle supervision of Lt. C oh. (Liter (Col.) Lan11-irete B. EHlis. MC...i’apelu ~s slowly lntd led t heimi to ittrttibu te lpiolperit es to thle drug t hiit it dloes riot posse’ss. Sutpplies of’ quinlimti were sniiiI in thle

The Russian Perception of the NATO Aerospace Threat: Could It Lead to Preemption

Science.gov (United States)

2016-09-01

led to the most serious crisis in Russian-American/Western relations since the end of the Cold War. So far, no one has suggested a plausible...potential for great danger in others. In circumstances that make Russian-American relations stable and reasonably businesslike, threat perceptions are...OtrasPublicaciones/ Internacional /2016/Russian-National-Security-Strategy-31Dec2015.pdf. 77. For the difficulties of fighting such a war, see Lt Col

Air Force Institute of Technology Research Report 2008

Science.gov (United States)

2009-05-01

Air Force Student, Summer 2008, Naval Postgraduate School, Monterey, CA. FIORINO, STEVEN, T., Lt Col Bartell, R.J., Krizo, M.J., Fiorino, S.T...Tomography. AFIT/GAP/ENP/08-M04. Faculty Advisor: Dr. William F. Bailey. Sponsor: AFRL/RZ. HAUSMAN , ALEXANDER R., Direct Initiation through...Sponsor: 314 OG/CC. MITCHELL, LAWRENCE W.S., Fee for Service Air Refueling: A Summary Market Analysis. AFIT/IMO/ENS/08-12. Faculty Advisor: Dr

Air & Space Power Journal. Volume 26, Number 5. September-October 2012

Science.gov (United States)

2012-10-01

of their air support through variations in doctrine or expansion of available options to meet the mission requirements. Envisioning mul­...Alfred Goldberg and Lt Col Donald Smith, Army–Air Force Relations: The Close Air Support Issue, R-906-PR (Santa Monica, CA: RAND, October 1971), 16...most impor­ tant near term priority” and recognizing it as “the most effective way to generate capacity, increase tempo , [and achieve] maximum

Increasing the Weaponization of Space: A Prescription for Further Progress

Science.gov (United States)

1998-04-01

Weidenheimer, Lt. Col., AF A Research Report Submitted to the Faculty In Partial Fulfillment of the Graduation Requirements Advisor : Dr William Martel Maxwell... robo -bugs), transatmospheric vehicles, and a ground-based laser system,” and defensive, using “miniature satellite body guards to protect high value...communications with Congress and other influential decision- makers and key advisors . DOD would likely play a supporting role in any communications with the

Report of the DHS Small Vessel Security Institute

Science.gov (United States)

2007-10-19

Local Government Interests: LtCol Don Holway; Florida Fish and Wildlife Conservation Commission Maj John Fetterman , Maine Department of Marine...AWW program to help fill gaps in law enforcement patrols. 68 National Small Vessel Security Summit Major John C. Fetterman – State of Maine...Major Fetterman currently serves as Vice President of the National Association of State Boating Law Administrators and for the last 30 years has been a

Purification of supercoiled DNA of plasmid Col E1 by RPC-5 chromatography

Energy Technology Data Exchange (ETDEWEB)

Best, A.N.; Allison, D.P.; Novelli, G.D.

1981-07-01

Col E1 DNA can be purified to a high degree by RPC-5 chromatography of a partially purified cell lysate with a very shallow linear NaC1 gradient at pH 7.8. Electron micrographs demonstrated that the purest fractions were composed of 93% supercoiled (form I) DNA and 7% open circular (form II) DNA. The actual chromatography can be accomplished in 13 to 14 h and is designed for the production of several milligrams of plasmid DNA.

Inducible pathway is required for mutagenesis in Salmonella typhimurium LT2

International Nuclear Information System (INIS)

Orrego, C.; Eisenstadt, E.

1987-01-01

UV mutability of Salmonella typhimurium LT2 was eliminated in the presence of a multicopy plasmid carrying the Escherichia coli lexA + gene. This result suggests that inducible, SOS-like functions are required for UV mutagenesis in S. typhimurium. S. typhimurium strains carrying either point or deletion mutations in topA had previously been shown to lose their mutability by UV or methyl methanesulfonate. Mitomycin C induction of the Phi(mucB'-lacZ') fusion (a DNA damage-inducible locus carried on plasmid pSE205) in S. typhimurium topA was normal, suggesting that RecA is activated in topA mutants. These observations lead the authors deduce that S. typhimurium has at least one DNA damage-inducible locus in addition to recA that is required for UV mutability

The effect of rider weight and additional weight in Icelandic horses in tölt: part II. Stride parameters responses.

Science.gov (United States)

Gunnarsson, V; Stefánsdóttir, G J; Jansson, A; Roepstorff, L

2017-09-01

This study investigated the effects of rider weight in the BW ratio (BWR) range common for Icelandic horses (20% to 35%), on stride parameters in tölt in Icelandic horses. The kinematics of eight experienced Icelandic school horses were measured during an incremental exercise test using a high-speed camera (300 frames/s). Each horse performed five phases (642 m each) in tölt at a BWR between rider (including saddle) and horse starting at 20% (BWR20) and increasing to 25% (BWR25), 30% (BWR30), 35% (BWR35) and finally 20% (BWR20b) was repeated. One professional rider rode all horses and weight (lead) was added to saddle and rider as needed. For each phase, eight strides at speed of 5.5 m/s were analyzed for stride duration, stride frequency, stride length, duty factor (DF), lateral advanced placement, lateral advanced liftoff, unipedal support (UPS), bipedal support (BPS) and height of front leg action. Stride length became shorter (Y=2.73-0.004x; P0.05). In conclusion, increased BWR decreased stride length and increased DF proportionally to the same extent in all limbs, whereas BPS increased at the expense of decreased UPS. These changes can be expected to decrease tölt quality when subjectively evaluated according to the breeding goals for the Icelandic horse. However, beat, symmetry and height of front leg lifting were not affected by BWR.

Asymptomatic parental mosaicism for osteogenesis imperfect associated with a new splice site mutation in COL1A2

DEFF Research Database (Denmark)

Frederiksen, Anja Lisbeth; Dunø, Morten; Johnsen, Iben Birgit Gade

2016-01-01

Recurrent lethal perinatal osteogenesis imperfecta may result from asymptomatic parental mosaicism. A previously unreported mutation in COL1A2 leads to recurrent cases of fetal osteogenesis imperfecta Sillence type IIA, which emphasizes the importance of clinical and genetic evaluation of mosaicism...

Genetic associations of body composition, flexibility and injury risk with ACE, ACTN3 and COL5A1 polymorphisms in Korean ballerinas

Science.gov (United States)

Kim, Jun Ho; Jung, Eun Sun; Kim, Chul-Hyun; Youn, Hyeon; Kim, Hwa Rye

2014-01-01

[Purpose] The purpose of this study was to exam the association of body composition, flexibility, and injury risk to genetic polymorphisms including ACE ID, ACTN3 RX, and COL5A1 polymorphisms in ballet dancers in Korea. [Methods] For the purpose of this study, elite ballerinas (n = 97) and normal female adults (n = 203) aged 18 to 39 were recruited and these participants were tested for body weight, height, body fat, fat free mass, flexibility, injury risks on the joints and gene polymorphisms (ACE, ACTN3, COL5A1 polymorphism). [Results] As results, the ACE DD genotype in ballerinas was associated with higher body fat and percentage of body fat than the ACE II and ID genotypes (p sports injuries, the ankle injury of the XX-genotyped ballerinas was in significantly more prevalence than the RR and XX-genotyped ballerinas (p < 0.05). According to the odd ratio analysis, XX-genotyped ballerinas have the injury risk on the ankle about 4.7 (95% CI: 1.6~13.4, p < 0.05) times more than the RR and RX-genotyped ballerinas. Meanwhile, the COL5A1 polymorphism in ballerinas has no association with any factors including flexibility and injury risks. [Conclusion] In conclusion, ACE polymorphism and ACTN3 polymorphism were associated with ballerinas' performance capacity; COL5A1 was not associated with any factors of performance of Ballerinas. The results suggested that the ACE DD genotype is associated with high body fat, the ACTN3 XX genotype is associated with low fat-free mass, low flexibility, and higher risk of ankle-joint injury. PMID:25566457

One Hundred Years of Flight: USAF Chronology of Significant Air and Space Events, 1903-2002

Science.gov (United States)

2003-01-01

the day: 852 feet in 59 seconds. The brothers launched the airplane from a monorail track against a wind blowing slightly more than 20 miles per hour...No. 1, producing a tricycle landing gear and elim- inating the need for a launching rail or catapult. August 20: The Army’s Lt. Jacob Fickel fired a...flight, reconnoitering Turkish positions in Libya. That same day, another Italian airplane became the first to be hit by ground fire . 8 1911-12

Rotational-mode component of the density of levels of nuclei with A approx-lt 150

International Nuclear Information System (INIS)

Rastopchin, E.M.; Svirin, M.I.; Smirenkin, G.N.

1992-01-01

Some difficulties which arise in the use of the generalized superfluid model to describe the density of levels in the region A approx-lt 150, as the result of an imperfect understanding of collective nuclear excitations, are discussed. One possible way to overcome these difficulties is examined. The idea is to depart from the conventional classification of collective nuclear properties and make use of small static deformations predicted theoretically and a corresponding rotational-mode component of the density of levels of these nuclei

Improving containment mass and energy releases for CONTEMPT-LT/028 TU with RELAP5/MOD3

International Nuclear Information System (INIS)

DaSilva, H.C.; Choe, W.G.

1996-01-01

In order to obtain boundary conditions for RELAP5/MOD3 best estimate (BE) large break (LB) loss-of-coolant accident (LOCA) calculations, it is necessary to utilize a separate containment analysis code CONTEMPT-LT/028 TU, which in turn accepts mass and energy releases from the RELAP5/MOD3 calculation. When these boundary conditions are obtained, they are observed to be significantly lower than those reported in FSAR containment analyses. This motivates the present study, where RELAP5/MOD3 mass and energy releases are generated using the same assumptions listed in the FSAR containment calculations. Then CONTEMPT-LT/028 TU pressures and temperatures calculated with both sets of mass and energy releases are compared. It is seen that those obtained with the RELAP5/MOD3 input are still significantly lower, indicating a level of conservatism in the FSAR mass and energy releases that is even above that explicitly listed and also incorporated into the RELAP5/MOD3 calculation. An important conclusion from this finding is that Environmental Qualification (EQ) issues requiring containment re-analyses are likely to be easily resolved if new mass and energy releases are calculated with state-of-the-art LOCA codes modeling the entire reactor coolant system, even when conservative assumptions are incorporated

X-Ray Diffraction Contrast Tomography in micro-CT Lab Source Systems

Science.gov (United States)

2014-05-16

Manager : Dr. Randall ’Ty’ Pollak, Lt Col, PhD International Program Officer EOARD Materials & Nanotechnology Office: +44-1895616115 (DSN 314-235-61... principles of this method can also be used to improve the plane normal estimations, which is likely to lead to sharper recon- structions of individual...grain volume. In practise , this condition is rarely fulfilled and already small deviations of order of a few tenths of a degree of intragranular

A Cooperative Communication System for the Advancement of Safe, Effective, and Efficient Patient Care

Science.gov (United States)

2014-09-01

As more data becomes available to the AI system during patient care, the CCS will continuously (in real time) improve its availability and...available via a relational database. To that end, ARA developed a customizable widget-based web framework for use in CCS. Users will be presented with a...Manager Dr. Salinas, LtCol Pamplin, and Ms. Mario -Selvin to discuss Phase 2. 4. Results. The research team developed a number of work in-progress

Understrength Air Force Officer Career Fields. A Force Management Approach

Science.gov (United States)

2005-01-01

LtCol John Crown (DPSA). In addition, we had very helpful interviews with Mr. Vaughan Blackstone (DPAPP) and Mr. Dennis Miller (DPPAO). Also at...problems in managing personnel assignments. First, there is a high " tax " for special-duty jobs that requires them to place personnel officers into...targeted year-groups populated above the ideal force- structure line (called TOPLINE), in the run up to the RIF of 1992, the desire to avoid or

Office of Strategic Services Training during World War II

Science.gov (United States)

2010-06-01

States: centralized intelligence, “fifth column” activities, psycho- logical or “ political warfare,” and the kind of sabotage, com- mando raids and...ship of Lt. Col. Serge Obolen- sky, a former Russian prince and New York socialite who had fought the Germans in World War I, the Bolsheviks in...and conflicting political agendas. Some veter- ans grumbled about undue emphasis on “cloak and dagger creepiness” instead of practical training that

JPRS Report, Soviet Union, Aviation & Cosmonautics, No. 5, May 1988

Science.gov (United States)

1988-10-28

onions, dill). High- fiber foods and foods which promote gas ( peas , beans, radishes, kvas, melons) should not be consumed. Prevention of discomfort...Mas- ter of Sport USSR Lt Col L. Kovalev, and repeated world and USSR record holder Maj V. Perminov, inspected the jumpers. The calm tone of...conducted in two areas. One involved the growing of large homoge- neous protein crystals on the Aynur unit. Essentailly these were the first Soviet

Desenvolvimento de colônias de abelhas com diferentes alimentos protéicos Development of honeybee colonies under protein diets

Directory of Open Access Journals (Sweden)

Fábia de Mello Pereira

2006-01-01

Full Text Available O objetivo deste trabalho foi avaliar o desempenho de produtos regionais do Nordeste na alimentação de colônias de abelhas (Apis mellifera, em um período de escassez de floradas. Foram fornecidas dietas às abelhas, contendo 20% de proteína bruta, à base de feno de mandioca (Manihot esculenta e farinha de vagem de algaroba (Prosopis juliflora, feno de mandioca e farelo de babaçu (Orbygnia martiana, farelo de babaçu e Purilac (sucedâneo para bezerros da marca Purina e pólen apícola de Palmae. As colônias foram analisadas quanto ao peso e às áreas de alimento e cria. Não foi observada diferença significativa entre os tratamentos em relação às áreas de cria. Apesar de a pasta com pólen ser a mais consumida, este alimento mostrou conversão alimentar menor do que as demais dietas fornecidas. As colônias que receberam pasta de feno de mandioca com farelo de babaçu tiveram maior peso final. Todos os alimentos mostraram-se eficientes na manutenção das colônias.The objective of this work was to evaluate the efficiency of some regional products of Brazil Northeast to feed Apis mellifera colonies. Diets with 20% of crude protein made of cassava hay (Manihot esculenta and mesquite pod meal (Prosopis juliflora, cassava hay and babassu bran (Orbygnia martiana, babassu bran and Purilac (succedaneous for calfskin from Purina and Palmae pollen were offered to the honeybees. Colonies were evaluated for weight gain, store area and brood area. There was no significant difference among the treatments in relation to the brood areas. Pollen treatment showed the highest intake but also showed the lowest food conversion. Beehives that received diet with cassava hay and babassu flour showed greater final weight gain. All diets were efficient in the maintenance of the colonies.

On the Solution of the Rational Matrix Equation X=Q+LX−1LT

Directory of Open Access Journals (Sweden)

Heike Faßbender

2007-01-01

Full Text Available We study numerical methods for finding the maximal symmetric positive definite solution of the nonlinear matrix equation X=Q+LX−1LT, where Q is symmetric positive definite and L is nonsingular. Such equations arise for instance in the analysis of stationary Gaussian reciprocal processes over a finite interval. Its unique largest positive definite solution coincides with the unique positive definite solution of a related discrete-time algebraic Riccati equation (DARE. We discuss how to use the butterfly SZ algorithm to solve the DARE. This approach is compared to several fixed-point and doubling-type iterative methods suggested in the literature.

Perdas de colônias de Apis mellifera L. no inverno suplementadas com alimentação artificial com pólen e favos de mel

Directory of Open Access Journals (Sweden)

Adhemar Pegoraro

2012-11-01

Full Text Available O objetivo deste trabalho foi avaliar o efeito do tipo de suplementação, nas áreas de cria, alimento e infestação por Varroa destructor nas colônias perdidas. Foram analisadas18 colônias de Apis mellifera L. alojadas em colméias modelo Langstroth e submetidas a dois tipos de suplementação alimentar: 1- cremosa (açúcar cristal moído, mel e pólen; 2- favos de mel. As áreas de ovo-larva e pupa, néctar, mel e pólen das 18 colônias foram fotografadas e analisadas com o auxílio do software AutoCAD. As colônias perdidas suplementadas com alimento artificial e pólen possuíam áreas de: ovo-larva (87,79 ± 70,38cm2, pupa (228,46 ± 192,94cm2, néctar (1.219 ± 149,58cm2, mel (563,47 ± 807,93cm2, pólen (139,09 ± 80,30cm2 e percentagem de infestação por V. destructor (10,50 ± 4,30%. As colônias perdidas suplementadas com favos de mel possuíam áreas de ovo-larva (7,92 ± 9,35cm2, pupas (60,71 ± 63,94 cm2, néctar (658,22 ± 1.040,94cm2, mel (122,62 ± 49,45cm2, pólen (53,58 ± 72,39cm2 e percentagem de infestação por V. destructor (14,40 ± 1,56%. As causas das perdas em parte podem ser atribuídas à infestação por V. destructor.

Avaliação e monitoramento de implantes ósseos de arcabouços de PLDLA imobilizados com colágeno, préviamente cultivados com osteoblastos

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Paula Galhano Filler

2014-10-01

Full Text Available Introdução: Polímeros biorreabsorvíveis, como PLDLA, são amplamente empregados como arcabouços na engenharia tecidual óssea. Incentiva-se sua interação com macromoléculas naturais da matriz extracelular, como o colágeno, para melhor adesão e reconhecimento do material implantado, e maior neoformação óssea. Objetivo: Analisar e comparar a regeneração óssea através de implantes realizados na calota craniana de ratos. Metodologia: Utilizou-se ratos Wistar de aproximadamente 2 meses, e tempos de sacrifício de 8 e 12 semanas, divididos nos grupos: defeito vazio; PLDLA-Col sem osteoblastos; e PLDLA-Col com osteoblastos. Paralelamente, estudou-se a fluorescência PKH26 em animais de 4 semanas, para avaliar a capacidade dos arcabouços de suportar o crescimento celular in vitro, Resultados: A analise histológica dos tempos de 8 e 12 semanas demonstrou neoformação óssea acentuada e irregular nos implantes de PLDLA-Col com ou sem célula, e fechamento parcial da lesão na maioria dos casos. Além disso, observou-se crescimento ósseo sem relação com as bordas do defeito. A histomorfometria de ambos os tempos apresentou o seguinte resultado decrescente em relação a área óssea neoformada: tratamento > controle positivo > controle negativo. Conclusão: Arcabouços de PLDLA-Col, previamente ou não cultivados com osteoblastos, obtiveram resultados promissores quando comparados ao defeito vazio, confirmando sua importante utilidade na engenharia tecidual.

Synthesis and Characterization of Hydroxyapatite-Collagen-Chitosan (HA/Col/Chi) Composite Coated on Ti6Al4V

Science.gov (United States)

Charlena; Bikharudin, Ahmad; Wahyudi, Setyanto Tri

2018-01-01

HA-collagen-chitosan (HA/col/chi) composite is developed to increase bioactivity adhesiveness between the metal and the material composite and to improve corrosion resistance. The Ti6Al4V alloy was coated by soaking in HA/col/chi composite at room temperature and then allowed to stand for 5, 6, and 7 days. Diffraction pattern analysis of the coated Ti6Al4V alloy showed that the dominant phase were HA and Ti6Al4V alloy. Corrosion resistance test in media by using 0.9% NaCl showed the corrosion rate at the level of 0.3567 mpy, which was better than that of the uncoated Ti6Al4V alloy (0.4152 mpy). In vitro cytocompatibility assay on endothelial cell of calf pulmonary artery endothelium (CPAE) (ATCC-CCL 209) showed there was no toxicity in the cell culture with the percent inhibition of 33.33% after 72 hours of incubation.

Biblioteca do Colégio Estadual Manoel Vilaverde: um espaço além das quatro paredes

Directory of Open Access Journals (Sweden)

Maria Aparecida Rodrigues de Souza

2005-07-01

Full Text Available Relato de experiência da prestação de serviços e atividades de leitura desenvolvidas na Biblioteca Domingos Garcia Filho do Colégio Estadual Manoel Vilaverde, em Inhumas – Goiás. Este relato faz um apanhado das estratégias para estímulo à leitura praticadas pela BDGF desde a sua reativação em 2001; ano em que o colégio foi contemplado com mais de 1300 livros pelo Programa de Bibliotecas das Escolas Estaduais do Estado de Goiás. Ilustramos através de fotos a participação efetiva dos alunos nos projetos desenvolvidos pela biblioteca. Durante o período de 2001 a 2004 foram desenvolvidos dois grandes projetos: Minutos de Leitura e o Projeto Pró-melhoria dos recursos pedagógicos do CEM. Projetos estes que envolvem diretamente a biblioteca, responsável pela mediação do usuário com a leitura e a pesquisa.

Genetic fusions of a CFA/I/II/IV MEFA (multiepitope fusion antigen) and a toxoid fusion of heat-stable toxin (STa) and heat-labile toxin (LT) of enterotoxigenic Escherichia coli (ETEC) retain broad anti-CFA and antitoxin antigenicity.

Science.gov (United States)

Ruan, Xiaosai; Sack, David A; Zhang, Weiping

2015-01-01

Immunological heterogeneity has long been the major challenge in developing broadly effective vaccines to protect humans and animals against bacterial and viral infections. Enterotoxigenic Escherichia coli (ETEC) strains, the leading bacterial cause of diarrhea in humans, express at least 23 immunologically different colonization factor antigens (CFAs) and two distinct enterotoxins [heat-labile toxin (LT) and heat-stable toxin type Ib (STa or hSTa)]. ETEC strains expressing any one or two CFAs and either toxin cause diarrhea, therefore vaccines inducing broad immunity against a majority of CFAs, if not all, and both toxins are expected to be effective against ETEC. In this study, we applied the multiepitope fusion antigen (MEFA) strategy to construct ETEC antigens and examined antigens for broad anti-CFA and antitoxin immunogenicity. CFA MEFA CFA/I/II/IV [CVI 2014, 21(2):243-9], which carried epitopes of seven CFAs [CFA/I, CFA/II (CS1, CS2, CS3), CFA/IV (CS4, CS5, CS6)] expressed by the most prevalent and virulent ETEC strains, was genetically fused to LT-STa toxoid fusion monomer 3xSTaA14Q-dmLT or 3xSTaN12S-dmLT [IAI 2014, 82(5):1823-32] for CFA/I/II/IV-STaA14Q-dmLT and CFA/I/II/IV-STaN12S-dmLT MEFAs. Mice intraperitoneally immunized with either CFA/I/II/IV-STa-toxoid-dmLT MEFA developed antibodies specific to seven CFAs and both toxins, at levels equivalent or comparable to those induced from co-administration of the CFA/I/II/IV MEFA and toxoid fusion 3xSTaN12S-dmLT. Moreover, induced antibodies showed in vitro adherence inhibition activities against ETEC or E. coli strains expressing these seven CFAs and neutralization activities against both toxins. These results indicated CFA/I/II/IV-STa-toxoid-dmLT MEFA or CFA/I/II/IV MEFA combined with 3xSTaN12S-dmLT induced broadly protective anti-CFA and antitoxin immunity, and suggested their potential application in broadly effective ETEC vaccine development. This MEFA strategy may be generally used in multivalent

Genetic fusions of a CFA/I/II/IV MEFA (multiepitope fusion antigen and a toxoid fusion of heat-stable toxin (STa and heat-labile toxin (LT of enterotoxigenic Escherichia coli (ETEC retain broad anti-CFA and antitoxin antigenicity.

Directory of Open Access Journals (Sweden)

Xiaosai Ruan

Full Text Available Immunological heterogeneity has long been the major challenge in developing broadly effective vaccines to protect humans and animals against bacterial and viral infections. Enterotoxigenic Escherichia coli (ETEC strains, the leading bacterial cause of diarrhea in humans, express at least 23 immunologically different colonization factor antigens (CFAs and two distinct enterotoxins [heat-labile toxin (LT and heat-stable toxin type Ib (STa or hSTa]. ETEC strains expressing any one or two CFAs and either toxin cause diarrhea, therefore vaccines inducing broad immunity against a majority of CFAs, if not all, and both toxins are expected to be effective against ETEC. In this study, we applied the multiepitope fusion antigen (MEFA strategy to construct ETEC antigens and examined antigens for broad anti-CFA and antitoxin immunogenicity. CFA MEFA CFA/I/II/IV [CVI 2014, 21(2:243-9], which carried epitopes of seven CFAs [CFA/I, CFA/II (CS1, CS2, CS3, CFA/IV (CS4, CS5, CS6] expressed by the most prevalent and virulent ETEC strains, was genetically fused to LT-STa toxoid fusion monomer 3xSTaA14Q-dmLT or 3xSTaN12S-dmLT [IAI 2014, 82(5:1823-32] for CFA/I/II/IV-STaA14Q-dmLT and CFA/I/II/IV-STaN12S-dmLT MEFAs. Mice intraperitoneally immunized with either CFA/I/II/IV-STa-toxoid-dmLT MEFA developed antibodies specific to seven CFAs and both toxins, at levels equivalent or comparable to those induced from co-administration of the CFA/I/II/IV MEFA and toxoid fusion 3xSTaN12S-dmLT. Moreover, induced antibodies showed in vitro adherence inhibition activities against ETEC or E. coli strains expressing these seven CFAs and neutralization activities against both toxins. These results indicated CFA/I/II/IV-STa-toxoid-dmLT MEFA or CFA/I/II/IV MEFA combined with 3xSTaN12S-dmLT induced broadly protective anti-CFA and antitoxin immunity, and suggested their potential application in broadly effective ETEC vaccine development. This MEFA strategy may be generally used in

Comparison of “Morning Olifera Seed Extract and Magnafloc LT25” in Removal of Water Turbidity Case Study: Tehran’s Jalalieh (1 Water Treatment Plant

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Mehdi Khan ahmadi

2013-08-01

Full Text Available Coagulation-flocculation is one of the most important processes for water treatment, commonly used all over the world. The use of synthetic polymers known as “Polyelectrolytes as a coagulant aid is now wide spread in water treatment plants. Tehran’s “Jalalieh WTP” has been using an anionic polyelectrolyte under the trade name of “Magna Floc LT25” for a number of years MagnaFloc LT25 (MF-LT25 is a synthetic coagulant with high molecular weight, produced in granular and powder form. The application of this coagulant aid is known to be most effective if injected to water after addition of main coagulant (Ferric Chloride in Tehran’s WTP.  However the use of synthetic polymers in treatment of drinking water has always been under question due to extract of MO seed contains proteins soluble in water which have high coagulating properties. This pilot scale study was performed to compare the coagulating properties of seed extract of MO to MF-LT25, The tests were carried out on Tehran’s raw water in Jalalieh treatment plant. Several turbidity ranges such as 5, 20, 100, and 200 NTU were used representing real seasonal turbidities of influent water to treatment plant. Optimum concentration of the coagulant and coagulant aid was determined within water pH values of 6, 7 and 8, using common “Jar Test” technique. The extract of Moringa Oleifera seed in optimum concentration of 10, 14,25,35 mg/L and optimum pH of 8 is capable in removal of 96.5, 97.4, 98.5 and 99% of water turbidity range of 5, 20, 100, and 200 NTU respectively. Under similar conditions, MF-LT25 removal efficiency were found 97, 98.1, 98.4 and 99.4% of water turbidity range of 5, 20, 100, and 200 NTU at optimum concentrations of 0.015, 0.02, 0.025, 0.03 mg/L respectively. The results also showed that the extract of Moringa Oleifera seed is a powerful coagulating agent, at alkalinity pH values, and is more effective for high turbid waters. It compared well with synthetic

Genetic Variations of the COL4A1 Gene and Intracerebral Hemorrhage Risk: A Case-Control Study in a Chinese Han Population.

Science.gov (United States)

Lin, Sen; Xia, Chao; He, Sha; Yang, Jie; Li, Hao; Zheng, Jun; Liu, Ming; You, Chao

2018-04-01

To investigate the association between single nucleotide polymorphisms or haplotypes of the COL4A1 gene and the risk of intracerebral hemorrhage (ICH). We conducted a case-control study that included 181 patients from the Chinese Han population with hypertensive ICH and 197 hypertension patients without ICH. Genomic DNA was extracted by DNA extraction kit, and the 6 single nucleotide polymorphism genotypes of the COL4A1 gene were detected with a MassARRAY Analyzer. Unphased 3.1.4 and SPSS 19.0 were used to analyze the association between alleles, genotypes, and haplotypes of the COL4A1 gene and the risk of ICH. Compared with the control group, patients in the ICH group were significantly younger. There were no differences in gender, diabetes, hyperlipidemia, current smoking, and alcohol consumption between the 2 groups. Our association analysis showed that the rs3742207 A, rs11069830 A, and rs679505 A alleles were association factors of the risks of ICH; rs11069830 AA, rs544012 AC, and rs679505 AA genotypes were association factors of the risk of ICH; AA haplotype (rs3742207-rs11069830) was an association factor of the risk of ICH. After adjusting age and gender by multivariate logistic regression, the rs544012 AC and rs679505 AA genotypes were independently associated with the risk of ICH. Our study showed that the rs544012 AC and rs679505 AA genotypes were independently associated with the risk of ICH in the Chinese Han population and that the AA haplotype (rs3742207-rs11069830) in the COL4A1 gene may be related to the risk of ICH in the Chinese Han population; these conclusions need further confirmation in future studies with larger samples. Copyright © 2018. Published by Elsevier Inc.

A familial case of achondrogenesis type II caused by a dominant COL2A1 mutation and "patchy" expression in the mosaic father.

Science.gov (United States)

Forzano, F; Lituania, M; Viassolo, A; Superti-Furga, V; Wildhardt, G; Zabel, B; Faravelli, F

2007-12-01

Achondrogenesis type II (ACG2) is the most severe disorder that can be produced by dominant mutations in COL2A1. We report on four pregnancies of an apparently healthy, nonconsanguineous young couple. The father had scoliosis as a child, and has slight body disproportion with short trunk. The first child was born at 32 weeks and died neonatally. In the second pregnancy, short limbs and fetal hygroma were noted on ultrasound at 17 weeks' gestation. Similar findings were observed in the third fetus. Clinical, radiological, and histological evaluation of the fetuses after termination of the pregnancies showed findings consistent with ACG2. Molecular analysis of genomic DNA extracted from amniotic cells of the second and third fetuses revealed heterozygosity for a 10370G > T missense mutation (G346V) in the COL2A1 gene. This mutation was also found in the father, as a mosaic. The couple had a fourth pregnancy, and at 11 weeks fetal hydrops with a septated cystic hygroma were obvious. DNA from CVS demonstrated the same COL2A1 mutation. (c) 2007 Wiley-Liss, Inc.

Uso da terra e perda de solo na Bacia Hidrográfica do Rio Colônia, Bahia Land use and soil loss in the Colônia River Watershed, Bahia

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Vinícius de A. Silva

2011-03-01

Full Text Available Mudanças no uso da terra muitas vezes potencializam a erosão hídrica acarretando perda de água, solo, nutriente e matéria orgânica dos sistemas agrícolas, razão por que se estimou a perda de solo na bacia hidrográfica do rio Colônia, na Bahia, nos últimos vinte e sete anos, utilizando-se o software SWAT (Soil and Water Assessment Tool. Para tal, procedeu-se à digitalização de mapas temáticos, interpretação de fotografias aéreas de 1975; classificação supervisionada de imagens de satélites de 2002 e produção de mapas de uso da terra. O SWAT foi utilizado na obtenção de mapas temáticos digitais por sub-bacia hidrográfica do rio Colônia, quantificação das perdas de solo em cada sub-bacia e nas formas de usos obtidos por conceito teórico, simulando as inclusões de áreas de preservação permanente, bem como mata em toda a superfície das sub-bacias. Estima-se que, entre 1975 e 2002, a média de perda de solo na bacia hidrográfica do rio Colônia foi de 47 t ha-1 ano-1 e em 2002 a estimativa de perda de solo foi de 46,64 t ha-1 ano-1. Na simulação de um cenário teórico de área de preservação permanente (APP e mata, ocorreu diminuição da média da perda de solo em toda a bacia hidrográfica do rio Colônia de, respectivamente, 9,09 t ha-1 ano-1 e 20,91 t ha-1 ano-1.Land use changes most of the time increases the hydric erosion leading to loss of water, nutrients and organic matter in agricultural systems. Thus, aiming to estimate the soil loss in the watershed of Colonia River, in Bahia, in the last twenty-seven years, the software SWAT (Soil and Water Assessment Tool was used. For the purpose, a digitalization thematic map (Arc View, interpretation of aerial photographs from 1975, supervised classification of 2002 satellite images and a land use map generation were developed. The SWAT software was used for obtaining a digital thematic map for every sub-basin of Colonia River Watershed, soil loss

A Novel Bifunctional Amino Acid Racemase With Multiple Substrate Specificity, MalY From Lactobacillus sakei LT-13: Genome-Based Identification and Enzymological Characterization

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Shiro Kato

2018-03-01

Full Text Available The Lactobacillus sakei strain LK-145 isolated from Moto, a starter of sake, produces potentially large amounts of three D-amino acids, D-Ala, D-Glu, and D-Asp, in a medium containing amylase-digested rice as a carbon source. The comparison of metabolic pathways deduced from the complete genome sequence of strain LK-145 to the type culture strain of Lactobacillus sakei strain LT-13 showed that the L- and D-amino acid metabolic pathways are similar between the two strains. However, a marked difference was observed in the putative cysteine/methionine metabolic pathways of strain LK-145 and LT-13. The cystathionine β-lyase homolog gene malY was annotated only in the genome of strain LT-13. Cystathionine β-lyase is an important enzyme in the cysteine/methionine metabolic pathway that catalyzes the conversion of L-cystathionine into L-homocysteine. In addition to malY, most genome-sequenced strains of L. sakei including LT-13 lacked the homologous genes encoding other putative enzymes in this pathway. Accordingly, the cysteine/methionine metabolic pathway likely does not function well in almost all strains of L. sakei. We succeeded in cloning and expressing the malY gene from strain LT-13 (Ls-malY in the cells of Escherichia coli BL21 (DE3 and characterized the enzymological properties of Ls-MalY. Spectral analysis of purified Ls-MalY showed that Ls-MalY contained a pyridoxal 5′-phosphate (PLP as a cofactor, and this observation agreed well with the prediction based on its primary structure. Ls-MalY showed amino acid racemase activity and cystathionine β-lyase activity. Ls-MalY showed amino acid racemase activities in various amino acids, such as Ala, Arg, Asn, Glu, Gln, His, Leu, Lys, Met, Ser, Thr, Trp, and Val. Mutational analysis revealed that the -amino group of Lys233 in the primary structure of Ls-MalY likely bound to PLP, and Lys233 was an essential residue for Ls-MalY to catalyze both the amino acid racemase and β-lyase reactions. In

Preparación, caracterización y aplicación como plataformas bioactivas de materiales compuestos formados por politiofeno y colágeno

OpenAIRE

Illera Bautista, Cristina

2014-01-01

El objetivo del trabajo es preparar mezclas compuestas por una proteína, en este caso colágeno tipo I, y un polímero conductor, poli[2,2’-(3-metil-acetato)tiofeno] (P3TMA), un derivado soluble de politiofeno. Para ello se ensayan diferentes metodologías para la preparación de las películas, tales como drop-casting y spin-coating. La proporción de proteína en las mezclas de colágeno/P3TMA es baja, variando entre el 5% y el 0,5% dependiendo de la técnica de preparación empleada, debido a la...

A inserção acadêmica e esportiva da primeira turma feminina no Colégio Militar do Rio de Janeiro

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Liliana Adiers Lohmann

2006-09-01

Full Text Available http://dx.doi.org/10.1590/S0104-026X2006000300005 Os objetivos deste trabalho são contextualizar o ambiente em que se deu a inserção da primeira turma feminina no Colégio Militar do Rio de Janeiro, em 1989, bem como identificar e analisar as representações femininas e masculinas sobre a inserção acadêmica e esportiva dessa primeira turma. A metodologia, qualitativa e etnográfica, fundamenta-se na teoria das representações sociais.1 Os dados provêm da observação participante e de seis entrevistas individuais não-diretivas de “elite”2: três ex-alunas e três homens. Analisam-se também reportagens de jornais e revistas, de 1989 a 1996. Constata-se que cuidado e diferença são constantes no discurso dos dois grupos de informantes. Conclui-se que o incentivo ao esporte, proposto pelo comandante do Colégio e liderado pela professora de Educação Física coautora deste trabalho, influenciou e consolidou o espaço para a participação e integração feminina no Colégio e para o seu êxito acadêmico e esportivo.

Collimation and material science studies (ColMat) at GSI.

CERN Document Server

Stadlmann, J; Kollmus, H; Krause, M; Mustafin, E; Petzenhauser, I; Spiller, P; Strasik, I; Tahir, N; Tomut, M; Trautmann, C

2010-01-01

Within the frame of the EuCARD program, the GSI Helmholtzzentrum für Schwerionenforschung in Darmstadt is performing accelerator R&D in workpackage 8: ColMat. The coordinated effort is focussed on materials aspects important for building the FAIR accelerator facility at GSI and the LHC upgrade at CERN. Accelerator components and especially protection devices have to be operated in high dose environments. The radiation hazard occurs either by the primary proton and ion beams or the secondary radiation after initial beam loss. Detailed numerical simulations have been carried out to study the damage caused to solid targets by the full impact of the LHC beam as well as the SPS beam. Tungsten, copper and graphite as possible collimator materials have been studied. Experimental an theoretical studies on radiation damage on materials used for the LHC upgrade and the FAIR accelerators are performed at the present GSI experimental facilities. Technical decisions based on these results will have an impact on the F...

Experimental plans for LMFBR cavity liner sodium spill test LT-1

International Nuclear Information System (INIS)

Hilliard, R.K.; Newell, G.A.

1976-01-01

Reinforced concrete is an important material of construction in LMFBR cavities and cells. Steel liners are often installed on the concrete surfaces to provide a gastight seal for minimizing air inleakage to inerted cell atmospheres and to protect the concrete from direct contact with sodium in the event of a sodium spill. In making safety assessment analyses, it is of interest to determine the adequacy of the liners to maintain their leaktightness during postulated accidents involving large sodium spills. However, data for basing analytical assessments of cell liners are very meager and an experimental program is underway at HEDL to provide some of the needed information. The HEDL cell liner evaluation program consists of both bench-scale feature tests and large-scale sodium spill demonstration tests. The plans for the first large-scale sodium spill test (LT-1) are the subject of this paper

Delineation of Ehlers-Danlos syndrome phenotype due to the c.934C>T, p.(Arg312Cys) mutation in COL1A1: Report on a three-generation family without cardiovascular events, and literature review.

Science.gov (United States)

Colombi, Marina; Dordoni, Chiara; Venturini, Marina; Zanca, Arianna; Calzavara-Pinton, Piergiacomo; Ritelli, Marco

2017-02-01

Classical Ehlers-Danlos syndrome (cEDS) is a rare connective tissue disorder primarily characterized by hyperextensible skin, defective wound healing, abnormal scars, easy bruising, and generalized joint hypermobility; arterial dissections are rarely observed. Mutations in COL5A1 and COL5A2 encoding type V collagen account for more than 90% of the patients so far characterized. In addition, cEDS phenotype was reported in a small number of patients carrying the c.934C>T mutation in COL1A1 that results in an uncommon substitution of a non-glycine residue in one Gly-Xaa-Yaa repeat of the pro-α1(I)-chain p.(Arg312Cys), which leads to disturbed collagen fibrillogenesis due to delayed removal of the type I procollagen N-propeptide. This specific mutation has been associated with propensity to arterial rupture in early adulthood; indeed, in literature the individuals harboring this mutation are also referred to as "(classic) vascular-like" EDS patients. Herein, we describe a three-generation cEDS family with six adults carrying the p.(Arg312Cys) substitution, which show a variable and prevalent cutaneous involvement without any major vascular event. These data, together with those available in literature, suggest that vascular events are not a diagnostic handle to differentiate patients with the p.(Arg312Cys) COL1A1 mutation from those with COL5A1 and COL5A2 defects, and highlight that during the diagnostic process the presence of at least the p.(Arg312Cys) substitution in COL1A1 should be investigated in cEDS patients without type V collagen mutations. Nevertheless, for these patients, as well as for those affected with cEDS, a periodical vascular surveillance should be carried out together with cardiovascular risk factors monitoring. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

The Temporal and Scalar Mechanics of Conflict: Strategic Implications of Speed and Time on the American Way of War

Science.gov (United States)

2006-03-15

variable speed” in terms of an operational pause? Just as Copernicus and Galileo ushered a paradigm shift into a world convinced that a geocentric ...Reimer’s POC, Lt Col John Medve, probably created and staffed the report for CSA, but it was signed by Gen. Reimer. The paper also noted that keeping the...and not geocentric (Earth-centered). His beliefs were in direct conflict with the religious and philosophical dogma of the period that saw Man as

The Effects of Student Narration in College Engineering Classes

Science.gov (United States)

2015-01-01

93(3), 223-231. 2004. 6. Norman, G., and Schmidt, H., “Effectiveness of Problem-Based Learning Curricula: Theory, Practice and Paper Darts ...conference papers . Dr. Richard Buckley is Lt Col in the U.S. Air Force and an Assistant Professor and Senior Military Faculty at the US Air Force...journal and conference papers . Dr. Dan Jensen is a Professor of Engineering Mechanics at the U.S. Air Force Academy where he has been since 1997. He

Interaction of Jet Fuel Hydrocarbon Components with Red Blood Cells and Hemoglobin

Science.gov (United States)

2014-06-24

Directorate (RHDJ), Wright-Patterson AFB, OH. The authors would like to thank Maj. Paul Eden, Nicole Schaeublin, Christin Grabinski, Dr. Jeff Gearhart...We would also like to thank LtCol. Norman Fox (Laboratory Flight Commander), Mrs. Nersa Loh (Supervisor, Transfusion Services), and Mr. Dan Fischer ...Approximately 7.8 mg of hemoglobin sample was concentrated into a total volume of 5 mL of Fischer PBS pH 7.5 buffer using an Amicon Centrifugal Filter Unit

Catalog of Air Force Weather Technical Documents 1941-2008

Science.gov (United States)

2008-06-19

Meteorological Rocketsonde Network, by Lt. Col. Walter I. Christensen, Maj. Terrell D. McCorry, and CMSgt. Ernest Fisher, June 1972, 24pp. Study presents...Biddulph, May 1989, 14pp. Inspired by Ann Besson, a reporter for the Kaiserslautern American, a newspaper that uses these summaries in a monthly “Weather...Asheville NC 28801-5002 DSN 673-9019. 4WW TM 70-2 (AD-None) Relationship Between 10 CM Solar Flux and Sunspot Number, by MSgt. Terrell S. Birch

2017 INFORMS PRIZE. The Nomination of The United States Air Force

Science.gov (United States)

2017-02-08

Member Lt Col Nicholas J. Zeisler Senior Operations Research Analyst, Headquarters Air Force (AF/A9RP) ROLE NAME ORGANIZATIONAL POSITION Publications...effective at detecting North Vietnamese trucks hidden under jungle canopy. Seventh Air Force Headquarters (top) Tan Son Nhut Air Base, Vietnam, 1962...1969 MACV Headquarters “Pentagon East” at Tan Son Nhut 432 0 17 I N F O R M S P R I Z E required the same kind of competent analysts, and applied the

Expressão citofotométrica da Caspase-3 no carcinoma papilífero da tireóide e no bócio colóide

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Luciana Rodrigues Queiroz de Souza

Full Text Available OBJETIVO: Descrever a expressão citofotométrica quantitativa do marcador caspase-3 no bócio colóide e no carcinoma papilífero da tireóide e comparar a imunoexpessão entre as doenças. MÉTODOS: Realizou-se estudo imunoistoquímico da proteína caspase-3 em 17 blocos de parafina de carcinoma papilífero da tiróide e 20 de bócio colóide, através do sistema SAMBA 4000 - (Sistema de análise microscópica de busca automática, objetivando-se analisar duas variáveis: índice de marcagem e densidade óptica. RESULTADOS: Houve diferença significativa quanto ao índice de marcagem da caspase-3, entre o bócio colóide e o carcinoma papilífero, sendo maior no carcinoma, e não foi encontrada diferença significativa quanto à densidade óptica. Para o bócio colóide, o coeficiente de correlação estimado entre o índice de marcagem e a densidade óptica foi igual a 0,72, indicando assim, a rejeição da hipótese nula (p <0,001, afirmando-se que existe associação positiva e significativa entre o índice de marcagem e a densidade óptica da caspase-3. Para o carcinoma papilífero da tiróide, o coeficiente de correlação estimado entre o índice de marcagem e a densidade óptica 3 foi de 0,34. O resultado do teste estatístico indicou que não se pode afirmar que existe associação entre esses parâmetros. CONCLUSÃO: Para o bócio colóide existe associação positiva e significativa entre as duas variáveis, índice de marcagem e a densidade óptica da caspase-3, enquanto que para o carcinoma papilífero não existe essa associação. O estudo comparativo entre a análise quantitativa da caspase-3, demonstrou que a apoptose é mais evidente no carcinoma papilífero do que no bócio colóide.

Clinical experience with EDAP LT-01+ extracorporeal shock wave lithotripsy for radiolucent stones; a report of 27 cases

International Nuclear Information System (INIS)

Rim, Hee Kwan; Kim, Ho Sung; Rim, Joung Sik

1994-01-01

Between February 1990 and February 1993, Radiolucent stones of 27 renal units in 22 patients were treated by extracorporeal shock wave lithotripsy (ESWL) using the EDAP LT-01+. Intravenous urography was done in all patients for the diagnosis of radiolucent stones. Retrograde pyelography, ultrasonography and/or computed tomography was done, if needed. The locations of stones were kidney in 11(40.7 %), upper ureter in 8(29.6 %), lower ureter in 6(22.2 %), bladder in 1(3.7 %), ureteropelvic junction in 1(3.7 %). The average stone size was 10.9mm with a range of 5 to 32mm in maximum diameter. The average numbers of treatment were 2.7 sessions and average storage required was 34.8 in one session. The average treatment time was 40.4 minutes. All the patients showed complete removal of all calculous materials. Of 17 urinary stones analysed by chemical method, 8(47.1 %) were composed of uric acid, 5(29.4 %) of uric acid and calcium, 3 of phosphate and 1 of carbonate and phosphate. Therefore, we conclude that ESWL with EDAP LT-01+ lithotriptor is considered to be an effective noninvasive procedure for treatment of radiolucent stones. (Author)

Acesso endoscópico transepto-interforniceal para cistos colóides: relato de caso

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VIALOGO JOSUÉ GUIMARÃES GRANHA

2000-01-01

Full Text Available A abordagem endoscópica de cistos colóides ainda é matéria controversa. As opções de tratamento são variadas: observação com exames seriados, tratamento da hidrocefalia, aspiração por estereotaxia, microcirurgia e endoscopia. O autor relata o caso de um paciente com cisto colóide do terceiro ventrículo, localizado em posição retroforaminal, no teto diencefálico, dissecando os dois fornices projetando-se supero-posteriormente em direção ao assoalho do ventrículo lateral, por dentro do cavum do septo pelúcido. A lesão foi alcançada com neuroendoscópio rígido, através de um orifício de trépano pré-coronal situado a 2,5 cm da linha média (ponto de Kocher. Seguindo por via transventricular, a lâmina direita do septo pelúcido foi aberta, posterior à veia septal. A lesão, muito endurecida, situada entre os dois fórnices, foi parcialmente ressecada através da via endoscópica transventricular transepto-interforniceal. Realizamos também septostomia e terceiro ventriculostomia endoscópicas neste paciente. Os diferentes tipos de procedimentos cirúrgicos das patologias situadas no terceiro ventrículo são discutidos com ênfase no papel da endoscopia.

Celularidade do leite e Unidades Formadoras de Colônias nas mastites causadas por Staphylococcus coagulase positiva e coagulase negativa

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Helio Langoni

2015-06-01

Full Text Available Resumo: A mastite é a principal afecção do gado destinado à produção leiteira, que impacta significativamente a cadeia produtiva do leite, com reflexos ainda para a saúde pública. Estudou-se aspectos relacionados à etiologia, celularidade e de contagem bacteriana em 10 propriedades leiteiras, localizadas no Estado de São Paulo. Foram examinadas 1148 vacas em lactação, totalizando 4584 glândulas mamárias. Foram considerados os casos, em que houve isolamento de estafilococos coagulase positiva (SCP e estafilococos coagulase negativa (SCN. Os resultados revelaram microbiota com vários patógenos e diferentes espécies de SCN (128 casos e SCP (45, Staphylococcus aureus(90, Streptococcus agalactiae(70, Streptococcus dysgalactiae (69, Streptococcus uberis(29, Corynebacteriumspp. (230, Klebsiella pneumoniae(28, Klebsiella oxytoca(2, Escherichia coli(15, Enterobactersp. (3. Os resultados de contagem de células somáticas (CCS relacionados aos SCP e SCN não mostraram diferenças entre as propriedades avaliadas, entretanto com diferenças significantes ao se avaliar a CCS entre os dois grupos de estafilococos, como pode ser evidenciado ao comparar SCN Discreto e SCP exuberante (P<0,01, SCP Discreto e SCP exuberante (P<0,001 e SCN moderado e SCP exuberante (P<0,01. A avaliação da CCS relacionada à intensidade da infecção, considerando-se como crescimento discreto o isolamento de até nove colônias, moderado de dez a 29 colônias e exuberante, com 30 ou mais colônias, revelou para ambos os grupos de estafilococos que quanto maior o número de unidades formadoras de colônias (UFC, a CCS é mais elevada, sendo sempre maior nos casos de SCP. Conclui-se que quando há maior número de UFC, há concomitantemente maior CCS/mL de leite, no caso dos SCP e SCN, o que mostra relação direta da intensidade do processo infeccioso com a resposta da celularidade do leite, bem como pela relevância desses na etiologia das mastites e dos aspectos

Analysis of four families with the Stickler syndrome by linkage studies. Identification of a new premature stop codon in the COL2A1 gene in a family

Energy Technology Data Exchange (ETDEWEB)

Bonaventure, J.; Lasselin, C. [Hopital Necker, Paris (France); Toutain, A. [CHU Bretonneau, Tours (France)] [and others

1994-09-01

The Stickler syndrome is an arthro-ophthalmopathy which associates progressive myopia with vitreal degeneration and retinal detachment. Cleft palate, cranio-facial abnormalities, deafness and osteoarthritis are often associated symptoms. Genetic heterogeneity of this autosomal dominant disease was consistent with its large clinical variability. Linkage studies have provided evidence for cosegregation of the disease with COL2A1, the gene coding for type II collagen, in about 50% of the families. Four additional families are reported here. Linkage analyses by using a VNTR located in the 3{prime} region of the gene were achieved. In three families, positive lod scores were obtained with a cumulative maximal value of 3.5 at a recombination fraction of 0. In one of these families, single strand conformation analysis of 25 exons disclosed a new mutation in exon 42. Codon for glutamic acid at position a1-803 was converted into a stop codon. The mutation was detected in DNA samples from all the affected members of the family but not in the unaffected. This result confirms that most of the Stickler syndromes linked to COL2A1 are due to premature stop codons. In a second family, an abnormal SSCP pattern of exon 34 was detected in all the affected individuals. The mutation is likely to correspond to a splicing defect in the acceptor site of intron 33. In one family the disease did not segregate with the COL2A1 locus. Further linkage studies with intragenic dimorphic sites in the COL10A1 gene and highly polymorphic markers close to the COL9A1 locus indicated that this disorder did not result from defects in these two genes.

Identification and characterization of the human type II collagen gene (COL2A1).

OpenAIRE

Cheah, Kathryn; Stoker, N.G.; Griffin, J.R.; Grosveld, Frank; Solomon, E.

1985-01-01

textabstractThe gene contained in the human cosmid clone CosHcol1, previously designated an alpha 1(I) collagen-like gene, has now been identified. CosHcol1 hybridizes strongly to a single 5.9-kilobase mRNA species present only in tissue in which type II collagen is expressed. DNA sequence analysis shows that this clone is highly homologous to the chicken alpha 1(II) collagen gene. These data together suggest that CosHcol1 contains the human alpha 1(II) collagen gene COL2A1. The clone appears...

Técnica da instilação de colírios em pacientes portadores de glaucoma crônico Eye drop instillation technique in chronic glaucoma patients

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Paulo Gelman Vaidergorn

2003-12-01

Full Text Available OBJETIVO: Verificar a técnica da instilação de colírio em pacientes portadores de glaucoma crônico. MÉTODOS: Estudo prospectivo realizado em 193 pacientes glaucomatosos. Para cada participante era entregue um frasco de colírio lubrificante (Dunason®, Laboratório Alcon, São Paulo, Brasil e solicitado que realizasse uma instilação. RESULTADOS: Os participantes utilizaram, em média, 1,64 ± 1,26 gotas de colírio por instilação e 54,5% dos pacientes fizeram contato do bico do colírio com a superfície ocular. Em 3,1% das instilações nenhuma gota de medicamento atingiu o olho, com o paciente não se dando conta do fato. A oclusão do ponto lacrimal ou a manutenção do olho fechado por dois minutos após a instilação não foi realizada em 87,0% dos participantes, e 61,6% piscaram repetidas vezes imediatamente após instilar a droga. CONCLUSÕES: Verificou-se que a maior parte dos participantes deste estudo efetuou a instilação do colírio de modo incorreto. Isto significa desperdiçar grande parte do conteúdo do frasco, aumentar as possibilidades de toxicidade sistêmica, não aproveitar a plenitude do efeito hipotensor das drogas e contaminar a extremidade do frasco de colírio. Portanto, o ensino da técnica adequada da instilação de colírio é absolutamente necessária para todos os pacientes.PURPOSE: To observe eye drop instillation technique in chronic glaucoma patients. METHODS: This is a prospective study enrolling 193 glaucomatous patients. Each received a sample of lubricant eye drop bottle (Dunason®, Alcon Laboratories, São Paulo, Brazil and was instructed to use it once. RESULTS: All patients used a mean amount of 1.64 ± 1.26 drops. In 54.5% of the patients, the eyedropper touched the eye and adnexa. In 3.1% the drops were not placed in the eye, without the patient being aware of this. In 87.0% of them, neither the lacrimal point occlusion nor the closure of the eyes for two minutes after instillation were

Dual reporter transgene driven by 2.3Col1a1 promoter is active in differentiated osteoblasts

Science.gov (United States)

Marijanovic, Inga; Jiang, Xi; Kronenberg, Mark S.; Stover, Mary Louise; Erceg, Ivana; Lichtler, Alexander C.; Rowe, David W.

2003-01-01

AIM: As quantitative and spatial analyses of promoter reporter constructs are not easily performed in intact bone, we designed a reporter gene specific to bone, which could be analyzed both visually and quantitatively by using chloramphenicol acetyltransferase (CAT) and a cyan version of green fluorescent protein (GFPcyan), driven by a 2.3-kb fragment of the rat collagen promoter (Col2.3). METHODS: The construct Col2.3CATiresGFPcyan was used for generating transgenic mice. Quantitative measurement of promoter activity was performed by CAT analysis of different tissues derived from transgenic animals; localization was performed by visualized GFP in frozen bone sections. To assess transgene expression during in vitro differentiation, marrow stromal cell and neonatal calvarial osteoblast cultures were analyzed for CAT and GFP activity. RESULTS: In mice, CAT activity was detected in the calvaria, long bone, teeth, and tendon, whereas histology showed that GFP expression was limited to osteoblasts and osteocytes. In cell culture, increased activity of CAT correlated with increased differentiation, and GFP activity was restricted to mineralized nodules. CONCLUSION: The concept of a dual reporter allows a simultaneous visual and quantitative analysis of transgene activity in bone.

Çokkültürlü Eğitim ve Öğretim Yeterliğine İlişkin Teorik Bir Çözümleme

OpenAIRE

KILIÇOĞLU, Gökhan

2015-01-01

Yirminci yüzyılın yılların başlarından bu yana göç hareketleriyle etnik çeşitliliği artan dünya günümüzde küreselleşmekte ve birçok farklılığı içerisinde barındırır hale gelmektedir. Dolayısıyla küreselleşen dünya, öğretmenlerin farklı kültürel geçmişe sahip olan öğrencilerin geçmişlerini ve deneyimlerini göz önünde bulundurarak çokkültürlü ve evrensel bakış açısına sahip olmalarını gerektirmektedir. Özellikle farklı kültürleri içerisinde barındıran toplumlar, farklılıklara sahip öğrenciler a...

Mardin Süryanilerinde Belleğin Teolojik-Kültürel Çerçevesi / Theological - Cultural Framework of Memory in Mardin Syriacs

Directory of Open Access Journals (Sweden)

Tahir Pekasil

2016-05-01

Full Text Available Theological - Cultural Framework of Memory in Mardin Syriacs Abstract In this article, “theological/ritual-cultural framework of memory”, "memory participation tools" "recall figures" will be examined in the case of Mardin Syriac Orthodox Church. "Ritual participation" and "rhythmic repetition" which enables "theological-social inclusion" and "integration", also mobilizes remembering instruments and guarantees protection, revival and transfer of cultural memory. This also ensures continuation of sense of belonging. Priests who are accepted as "vicarious memory" enable participation to memory in church which is a "memory space" mediating to the "transmission of blessing and grace by ritual representation". Priests also by institutionalizing ceremonies and rituals which integrated with music, makes cultural meanings functional. The theme is discussed from the perspective of cultural memory and supported by face to face interviews. Mardin Süryanilerinde Belleğin Teolojik-Kültürel Çerçevesi Öz Bu yazıda, Mardin Süryani Ortodoks Kilisesi örneğinden hareketle “belleğin teolojik/ritüel- kültürel çerçevesi”, “belleğe katılım araçları”, “hatırlama figürleri” ele alınacaktır. “Teolojik ve toplumsal katılımı”, “bütünleşmeyi” sağlayan “ritüel katılım” ve “ritmik tekrar”, hatırlama araçlarını harekete geçirerek kültürel belleğin korunmasını, canlandırılmasını ve aktarılmasını garanti etmekte, aidiyetin devamını sağlamaktadır. Belleğe katılımı sağlayan “vekil hafıza” olarak kabul edilen papazlar, bir “hafıza mekânı” olan kilisede, “inayetin ritüel temsille aktarımına” aracılık yapmakta; “ritüel tasarım” yoluyla müzikle bütünleştirdikleri ayin ve ritüelleri kurumsallaştırarak kültürel anlamlara işlerlik kazandırmaktadırlar. Çalışma konusu, kültürel bellek perspektifinden ele alınmış ve mülakat örnekleri ile desteklenmiştir.

Caracterização de colágenos tipos I e III no estroma do carcinoma de células escamosas cutâneo em cães

Directory of Open Access Journals (Sweden)

S.A.O. Bedoya

2016-02-01

Full Text Available O carcinoma de células escamosas (CCE é uma neoplasia epitelial maligna que acomete cães e diversas outras espécies, incluindo a humana. O CCE afeta vários sítios anatômicos e pode desenvolver metástase. O objetivo deste estudo foi a caracterização das fibras de colágenos tipos I e III no estroma do CCE cutâneo de cães. Para este trabalho, utilizaram-se 44 amostras de pele incluídas em parafina e que tiveram prévio diagnóstico de CCE. As amostras foram processadas histologicamente e coradas com hematoxilina/eosina para confirmação do diagnóstico e classificação do grau de diferenciação tumoral e com a coloração histoquímica de picrosirius para observação dos colágenos tipos I e III. O colágeno tipo III mostrou maior expressão nos CCEs cutâneos bem diferenciados. O papel do colágeno do tipo III nas neoplasias não está bem esclarecido, e outros fatores além do grau de diferenciação celular podem estar envolvidos em sua expressão e determinar sua importância na biologia tumoral.

Thrombin generation by activated factor VII on platelet activated by different agonists. Extending the cell-based model of hemostasis

Directory of Open Access Journals (Sweden)

Herrera Maria

2006-04-01

Full Text Available Abstract Background Platelet activation is crucial in normal hemostasis. Using a clotting system free of external tissue factor, we investigated whether activated Factor VII in combination with platelet agonists increased thrombin generation (TG in vitro. Methods and results TG was quantified by time parameters: lag time (LT and time to peak (TTP, and by amount of TG: peak of TG (PTG and area under thrombin formation curve after 35 minutes (AUC→35min in plasma from 29 healthy volunteers using the calibrated automated thrombography (CAT technique. TG parameters were measured at basal conditions and after platelet stimulation by sodium arachidonate (AA, ADP, and collagen (Col. In addition, the effects of recombinant activated FVII (rFVIIa alone or combined with the other platelet agonists on TG parameters were investigated. We found that LT and TTP were significantly decreased (p 35min were significantly increased (p 35min (but not PTG when compared to platelet rich plasma activated with agonists in the absence of rFVIIa. Conclusion Platelets activated by AA, ADP, Col or rFVIIa triggered TG. This effect was increased by combining rFVIIa with other agonists. Our intrinsic coagulation system produced a burst in TG independent of external tissue factor activity an apparent hemostatic effect with little thrombotic capacity. Thus we suggest a modification in the cell-based model of hemostasis.

A novel COL11A1 mutation affecting splicing in a patient with Stickler syndrome.

Science.gov (United States)

Kohmoto, Tomohiro; Naruto, Takuya; Kobayashi, Haruka; Watanabe, Miki; Okamoto, Nana; Masuda, Kiyoshi; Imoto, Issei; Okamoto, Nobuhiko

2015-01-01

Stickler syndrome is a clinically and genetically heterogeneous collagenopathy characterized by ocular, auditory, skeletal and orofacial abnormalities, commonly occurring as an autosomal dominant trait. We conducted target resequencing to analyze candidate genes associated with known clinical phenotypes from a 4-year-old girl with Stickler syndrome. We detected a novel heterozygous intronic mutation (NM_001854.3:c.3168+5G>A) in COL11A1 that may impair splicing, which was suggested by in silico prediction and a minigene assay.

Polymorphisms within the COL5A1 gene and regulators of the extracellular matrix modify the risk of Achilles tendon pathology in a British case-control study.

Science.gov (United States)

Brown, Karryn L; Seale, Kirsten B; El Khoury, Louis Y; Posthumus, Michael; Ribbans, William J; Raleigh, Stuart M; Collins, Malcolm; September, Alison V

2017-08-01

Several genetic loci have been associated with risk of Achilles tendon pathology (ATP) within South African and Australian populations. The aim of this study was, therefore, to evaluate eight previously implicated genetic variants in an independent British population. A total of 130 asymptomatic controls (CON) and 112 participants clinically diagnosed with ATP comprising 87 individuals with chronic Achilles tendinopathy (TEN) and 25 with Achilles tendon ruptures (RUP) were included. All participants were genotyped for variants within the COL5A1, MIR608, IL-1β, IL-6 and CASP8 genes. Primary findings implicated COL5A1 and CASP8. Three inferred allele combinations constructed from COL5A1 rs12722, rs3196378 and rs71746744 were identified as risk modifiers. The T-C-D combination was associated with increased risk of ATP (P = 0.023) and RUP (P RUP (P = 0.011) and the C-C-D combination was associated with decreased risk of ATP (P = 0.011) and RUP (P = 0.004). The CASP8 rs3834129 DD genotype was associated with decreased risk of TEN (P = 0.020, odds ratio: 0.45, 95% confidence interval: 0.22-0.90) and the CASP8 I-G (rs3834129-rs1045485) inferred allele combination was associated with increased risk of TEN (P = 0.031). This study further highlights the importance of polymorphisms within COL5A1 and CASP8 in the aetiology of ATP.

Preliminary calculation with code CONTEMPT-LT for spray cooling tests with JAERI model containment vessel

International Nuclear Information System (INIS)

Tanaka, Mitsugu

1978-01-01

LWR plants have a containment spray system to reduce the escape of radioactive material to the environment in a loss-of-coolant accident (LOCA) by washing out fission products, especially radioiodine, and condensing the steam to lower the pressure. For carrying out the containment spray tests, pressure and temperature behaviour of the JAERI Model Containment Vessel in spray cooling has been calculated with computer program CONTEMPT-LT. The following could be studied quantitatively: (1) pressure and temperature raise rates for steam addition rate and (2) pressure fall rate for spray flow rate and spray heat transfer efficiency. (auth.)

’When You Get a Job to Do, Do It.’ The Airpower Leadership of Lt Gen William H. Tunner

Science.gov (United States)

2008-01-01

PAGE unclassified Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std Z39-18 Air University Stephen R. Lorenz, Lt Gen, Commander Air Force Doctrine...Collier, Richard. Bridge across the Sky. New York: Mc Graw - Hill, 1978. Combined Airlift Task Force. A Report on the Airlift Berlin Mis- sion. 572.101B, 26

Air & Space Power Journal. Volume 28, Number 3, May-June 2014

Science.gov (United States)

2014-06-01

Lt Col Jason Evenson, Maj Cyn- thia Wittnam, Maj Jason Haufschild, Capt Joe Rice , Capt D. Jerred Cooper, Capt Holden Leute, Capt Hudson Graham, Jim...thesis, Naval Postgraduate School, March 2011), 17–38, http://www.dtic.mil/cgi-bin/GetTRDoc?AD=ADA 543725. 55. MSgt Louis A. Arana-Barradas, “ Black ...Samuels point out, Even with the best of intentions, it is common to make surface-level, of- ten cosmetic , changes in the hope of alleviating the problem

Parallel Attack and the Enemy’s Decision Making Process

Science.gov (United States)

1998-04-01

Theory Coursebook , Vol 2, Sept 1997, p 365 8 Joint Publication 3-0, Doctrine for Joint Operations, 1 Feb 1995, p III-11 9 Gorrell, LtCol Edgar S., “The...Behavioral Strategies,” Journal of the American Statistical Association, Vol 90, Issue 432, December, 1995, p1137 3 Joint Publication 5-0, Doctrine for...Strategies,” Journal of the American Statistical Association, Vol 90, Issue 432, December, 1995, p1137 Allison, Graham T., “Conceptual Models and the Cuban

Research at USAFA 2013

Science.gov (United States)

2013-01-01

program in the country and to share that expertise with others,” said Carlisle . Research at USAFA 20138 aC ad eM y Ce nT eR fo R Un M an ne d ai RC...independent research project to refine imaging techniques with CCD and CMOS camera sensors . Mentored by Lt Col Mario Serna, the cadets leveraged...scholars and Permanent Professors at the USAFA to provide a more academically in-depth explanation for CCLD’s framework and its implications for

Gulf War Air Power Survey. Volume 3. Logistics and Support

Science.gov (United States)

1993-01-01

situations, fuel could have been air dropped to units In fifty-five gallon drums." Tactical airlift had Its most rewarding test after the air war...locations were Hickam AFB in Hawaii, Andersen AFB in Guam, and Diego Garcia. Planning had also includedSingapore, Malaysia , and Clark AB in the Philippines...Intvw, Maj Dell Rose, 1909 ARE•FW(P), I Jan 1991 (IRIS 008o9?,31), 1Ibid; Lir, Lt Col Thomas M. Beres, Commander 8 Special Operations Squadron, to

Joint Force Quarterly. Number 3, Winter 1993-94

Science.gov (United States)

1994-01-01

Winter 1993–94 This article is based on the winning entry in the 1992 LtCol Richard Higgins, USMC, memorial essay contest sponsored by the National War...TRANSCOM pledges to develop a new system that lives up to Winston Churchill’s dictum: “Victory is the beautiful bright coloured flower. Transport is...fighter wings 7 Reserve fighter wings 7 Reserve fighter wings 10 Reserve fighter wings Force Enchancements 1803 Ltrs & JW Rev 3/27/04 7:31 AM Page 107

Isolated Subclinical Hyperthyrotropinemia in Obese Children: Does Levothyroxine (LT4 Improve Weight Reduction during Combined Behavioral Therapy?

Directory of Open Access Journals (Sweden)

Pawel Matusik

2015-01-01

Full Text Available Objective. The study aim was to analyze whether anthropometrical parameters and TSH values in obese children with isolated subclinical hypothyroidism (IsHT treated with levothyroxine (LT4 and weight reduction program differ from those managed by dietary and behavior counselling only. Material and Methods. 51 obese children with IsHT, who were treated according to the same weight reduction program, were retrospectively analyzed. They were divided into two groups: Group 1, n=26, and Group 2, n=25, without or with LT4 therapy, respectively. Changes in anthropometrical (delta BMI z-score and hormonal (delta TSH status were analyzed at the first follow-up visit. Results. In both groups significant decrease of TSH and BMI z-score values were noted. TSH normalized in 80.9% of children from Group 1 versus 90.5% from Group 2, p = NS. Delta BMI z-score was insignificantly higher in Group 1 compared to Group 2. Delta TSH was significantly related to initial TSH level in children treated by lifestyle intervention program only. Conclusions. In obese children with sHT dietary-behavioral management intervention contributed to reduction of body mass index, irrespective of levothyroxine use. This finding suggests that moderately elevated levels of TSH are a consequence rather than cause of overweight and pharmacological treatment should be avoided.

Raman evidence of the formation of LT-LiCoO 2 thin layers on NiO in molten carbonate at 650°C

Science.gov (United States)

Mendoza, L.; Baddour-Hadjean, R.; Cassir, M.; Pereira-Ramos, J. P.

2004-03-01

The structural evolution of thin layers of Co 3O 4 elaborated on nickel-based substrates in the Li 2CO 3-Na 2CO 3 carbonate eutectic at 650 °C as a function of time immersion is reported. Raman microspectrometry has been applied in order to provide more information on the nature of the protective cobalt oxide layers. The typical Raman fingerprint of the LT-LiCoO 2 compound has been obtained, with four well defined bands at 449, 484, 590 and 605 cm -1, while XRD data are unable to distinguish the layered phase (HT) from the spinel one (LT). The mechanical stability of such films does not exceed 10 h in direct contact with the molten carbonate bulk at 650 °C; nevertheless, these conditions are much more corrosive than in a molten carbonate fuel cell (MCFC).

The expanding spectrum of COL2A1 gene variants IN 136 patients with a skeletal dysplasia phenotype.

Science.gov (United States)

Barat-Houari, Mouna; Dumont, Bruno; Fabre, Aurélie; Them, Frédéric Tm; Alembik, Yves; Alessandri, Jean-Luc; Amiel, Jeanne; Audebert, Séverine; Baumann-Morel, Clarisse; Blanchet, Patricia; Bieth, Eric; Brechard, Marie; Busa, Tiffany; Calvas, Patrick; Capri, Yline; Cartault, François; Chassaing, Nicolas; Ciorca, Vidrica; Coubes, Christine; David, Albert; Delezoide, Anne-Lise; Dupin-Deguine, Delphine; El Chehadeh, Salima; Faivre, Laurence; Giuliano, Fabienne; Goldenberg, Alice; Isidor, Bertrand; Jacquemont, Marie-Line; Julia, Sophie; Kaplan, Josseline; Lacombe, Didier; Lebrun, Marine; Marlin, Sandrine; Martin-Coignard, Dominique; Martinovic, Jelena; Masurel, Alice; Melki, Judith; Mozelle-Nivoix, Monique; Nguyen, Karine; Odent, Sylvie; Philip, Nicole; Pinson, Lucile; Plessis, Ghislaine; Quélin, Chloé; Shaeffer, Elise; Sigaudy, Sabine; Thauvin, Christel; Till, Marianne; Touraine, Renaud; Vigneron, Jacqueline; Baujat, Geneviève; Cormier-Daire, Valérie; Le Merrer, Martine; Geneviève, David; Touitou, Isabelle

2016-07-01

Heterozygous COL2A1 variants cause a wide spectrum of skeletal dysplasia termed type II collagenopathies. We assessed the impact of this gene in our French series. A decision tree was applied to select 136 probands (71 Stickler cases, 21 Spondyloepiphyseal dysplasia congenita cases, 11 Kniest dysplasia cases, and 34 other dysplasia cases) before molecular diagnosis by Sanger sequencing. We identified 66 different variants among the 71 positive patients. Among those patients, 18 belonged to multiplex families and 53 were sporadic. Most variants (38/44, 86%) were located in the triple helical domain of the collagen chain and glycine substitutions were mainly observed in severe phenotypes, whereas arginine to cysteine changes were more often encountered in moderate phenotypes. This series of skeletal dysplasia is one of the largest reported so far, adding 44 novel variants (15%) to published data. We have confirmed that about half of our Stickler patients (46%) carried a COL2A1 variant, and that the molecular spectrum was different across the phenotypes. To further address the question of genotype-phenotype correlation, we plan to screen our patients for other candidate genes using a targeted next-generation sequencing approach.

A crise Armada Colômbia-Equador no contexto da Guerra contra o Terrorismo Internacional

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TATIANA WAISBERG

2010-10-01

Full Text Available A recente crise armada entre Colômbia e Equador trouxe a América do Sul ao foco da mídia internacional. As FARC representam o maior barril de pólvora da América Latina, além de um determinante ator a ser combatido na luta contra o terrorismo internacional. Nesse sentido, o presente artigo busca analisar, sob o prisma da segurança e do direito internacional o papel das FARC em âmbito global e as maneiras segundo as quais elas são combatidas.

LT-CFB. Applications and follow-up. CP Kelco. Appendix 1. [Low Temperature Circulating Fluid Bed gasifier]; LT-CFB. Anvendelsesmuligheder og opfoelgning. CP Kelco. Bilag 1

Energy Technology Data Exchange (ETDEWEB)

NONE

2010-04-15

This appendix describes how LT-CFB advantageously can gasify residual products at the company CP Kelco's factory. CP Kelco produces the gelation, thickeners and stabilizers carrageenan and pectin from seaweed and citrus peel. Residues from the production consist of a fiber-containing biological material, which has a calorific value of dry matter about 15 to 20 MJ / kg. In addition, there are waste products from the wastewater treatment plant, which are also proposed re-used in the project. The report describes the economic analyses, the overall data and system design for two scenarios, whereas the description of the plant capacities, sizes and detailed data etc. are described only for one scenario. In addition to savings in the purchase of natural gas the gas production also results in a significant reduction in CO{sub 2} emissions, the product gas being characterized as CO{sub 2} neutral. Since CP Kelco is subject to the CO{sub 2} quota system the saved amount of CO{sub 2} emissions represents a significant value. In the economic analyses, the value of 1 ton of CO{sub 2} is set at DKK 100. (LN)

Kültürel Farkındalık Yaratma Açısından Sanat Eleştirisi Öğretimi

OpenAIRE

Tuna, Serdar

2011-01-01

Bu çalışmanın amacı, özellikle ilk ve ortaöğretim görsel sanatlar dersi içerisinde sanat eleştirisi öğretiminin öğrencilere görsel algı geliştirme, tasarım ilke ve elemanlarını öğrenme ve saptama, sözel ve yazınsal ifadeyi geliştirme gibi yararlarının yanı sıra, sanat yapıtlarında kullanılan imgeler ve simgelerden yararlanarak kendi kültürünün farkına varma ve farklı kültürlerin yaşam tarzlarını öğrenme konusunda da son derece faydalı olacağını ortaya koymaktır. İletişim araçlarının gelişmesi...

High performance ceria-bismuth bilayer electrolyte low temperature solid oxide fuel cells (LT-SOFCs) fabricated by combining co-pressing with drop-coating

KAUST Repository

Hou, Jie

2015-03-24

The Sm0.075Nd0.075Ce0.85O2-δ-Er0.4Bi1.6O3 bilayer structure film, which showed an encouraging performance in LT-SOFCs, was successfully fabricated by a simple low cost technique combining one-step co-pressing with drop-coating.

High performance ceria-bismuth bilayer electrolyte low temperature solid oxide fuel cells (LT-SOFCs) fabricated by combining co-pressing with drop-coating

KAUST Repository

Hou, Jie; Bi, Lei; Qian, Jing; Zhu, Zhiwen; Zhang, Junyu; Liu, Wei

2015-01-01

The Sm0.075Nd0.075Ce0.85O2-δ-Er0.4Bi1.6O3 bilayer structure film, which showed an encouraging performance in LT-SOFCs, was successfully fabricated by a simple low cost technique combining one-step co-pressing with drop-coating.

Çokkültürlülük Bağlamında Türkiye’de Çokkültürcü Medya ve TRT Kürdi / Özet: Çokkültürlülük kavramı uzun bir geçmişe sahip olsa da çokkültürcü anlayış ve politikalar anlamında modern sonrası zamana denk gelmektedir. Etnik azınlıklara ve yerlilere yönelik geçmiş politikaların artık etkisini yitirdiği bir dönemde ortaya çıkan çokkültürcülük yaklaşımı, günümüz açısından, alternatif yaklaşımlarla birlikte halen anılmakta ve uygulanmaktadır. Türkiye’de de, 1990’lı yıllarda çeşitli nedenlerle dile getirilen, ancak uygulama açısından 2000’li yıllarda örneklerini gördüğümüz çokkültürcü liberal politikalar, medyaya da yansımıştır. Bu nedenle çalışmada söz konusu çokkültürcü politikaların Türkiye özelinde, medya örneklerine, kamu hizmeti yayıncılık anlayışı çerçevesinde değinilecektir. Bu bağlamda Türkiye’deki ilk çokkültürlü kamu hizmeti yayıncılık örneği olan TRT KÜRDİ’nin açılmasıyla sonuçlanan sürece dikkat çekilecektir. Anahtar kelimeler: Çokkültürlülük, Çokkültürcülük, Türkiye, TRT, KÜRDİ. Multiculturalist Media and TRT Kurdi in Turkey in the Contex of Multiculturalism

Directory of Open Access Journals (Sweden)

Filiz AYDOĞAN BOSCHELE

2016-07-01

Full Text Available Çokkültürlülük kavramı uzun bir geçmişe sahip olsa da çokkültürcü anlayış ve politikalar anlamında modern sonrası zamana denk gelmektedir. Etnik azınlıklara ve yerlilere yönelik geçmiş politikaların artık etkisini yitirdiği bir dönemde ortaya çıkan çokkültürcülük yaklaşımı, günümüz açısından, alternatif yaklaşımlarla birlikte halen anılmakta ve uygulanmaktadır. Türkiye’de de, 1990’lı yıllarda çeşitli nedenlerle dile getirilen, ancak uygulama açısından 2000’li yıllarda örneklerini gördüğümüz çokkültürcü liberal politikalar, medyaya da yansımıştır. Bu nedenle çalışmada söz konusu çokkültürcü politikaların Türkiye özelinde, medya örneklerine, kamu hizmeti yayıncılık anlayışı çerçevesinde değinilecektir. Bu bağlamda Türkiye’deki ilk çokkültürlü kamu hizmeti yayıncılık örneği olan TRT KÜRDİ’nin açılmasıyla sonuçlanan sürece dikkat çekilecektir. / Multiculturalism notion has coincided with postmodern era with respect to multicultural sense and policies in the world, even though the notion itself has a long historical background. Emerged in an era where old policies toward ethnic minorities and natives have become obsolete, multicultural view is still recognized and applied hand in hand with alternative views. As a matter of debate in 1990s, while not put into practice until 2000s in Turkey, multicultural liberal policies have found themselves wide media coverage in recent years. Therefore this study aims to shed a light on the process of launching TRT KURDI as the first multiculturalist broadcasting example in Turkey through investigating media examples from Turkey within the public broadcasting frame.

The Voice of the Turtle is Heard Programs to Develop Military Writers in the Field of Strategy

Science.gov (United States)

1966-04-08

BENEFIT TO THE USER AS MAY ACCRUE. 8 April 1966 "THE VOICE OF THE TURTLE IS HEARD" PROGRAMS TO DEVELOP MILITARY WRITERS IN THE FIELD OF STRATEGY By...U USAWC RESEARCH ELEMENT (Research Paper) L’The Voice of the Turtle is Heard" Programs to Develop Military Writers in the Field of Strategy by Lt Col...extensively their own "original sources" of information. Such information as published is often nebulous , however, and as often fanciful as it is true

Taxa de Infestação da Varroa destructor em colônias de Apis mellifera L. no Agreste Meridional de Pernambuco

Directory of Open Access Journals (Sweden)

Deygnon Cavalcanti Clementino

2016-08-01

Full Text Available O presente trabalho teve como objetivo verificar a taxa de infestação da Varroa destructor em colônias de Apis mellifera L. proveniente de apiário no município de Lagoa do Ouro, Agreste Meridional de Pernambuco. A pesquisa foi realizada no mês de setembro de 2015. Participaram do estudo 15 colônias, em que foram coletadas em média 227 abelhas adultas de cada colmeia e acondicionadas em recipientes contendo álcool a 70%. Após as coletas a campo, as amostras foram transportadas para o laboratório de Biologia Animal da Universidade Federal Rural de Pernambuco/Unidade Acadêmica de Garanhuns, para a realização das análises e quantificação dos ácaros. Os resultados demonstraram que 100% das amostras estavam parasitadas com o ácaro, sendo que a média geral da taxa de infestação foi de 6,16%. O grau de infestação pela Varroa destructor observado nas colônias variou de 0,93% a 11,15%. Conclui-se que as colônias analisadas apresentam diferentes níveis de infestação pelo ácaro Varroa destructor.Infestation rate of Varroa destructor in Apis mellifera L. colonies in the South Agreste of PernambucoAbstract: This study aimed to verify the infestation rate of Varroa destructor in Apis mellifera L. colonies in the apiary from Lagoa do Ouro County, South Agreste of Pernambuco. The survey was conducted in September 2015. The study included 15 colonies, which were collected on average 227 adult bees in each hive and placed in vials containing 70% alcohol. After harvesting the field, the samples were transported to the Animal Biology Laboratory at the Federal Rural University of Pernambuco/Academic Unit of Garanhuns, to perform the analysis and quantification of mites. The results showed that 100% of the samples were infested with mites, and the overall average infestation rate was 6.16%. The degree of infestation by Varroa destructor observed in colonies ranged from 0.93% to 11.15%. It is concluded that the analyzed colonies have

Characterization and Validation of the LT-SYS Copper Assay on a Roche Cobas 8000 c502 Analyzer.

Science.gov (United States)

Kraus, F Bernhard; Mischereit, Marlies; Eller, Christoph; Ludwig-Kraus, Beatrice

2017-02-01

Validation of the LT-SYS quantitative in vitro copper assay on a Roche Cobas 8000 c502 analyzer and comparison with a BIOMED assay on a Roche Cobas Mira analyzer. Imprecision and bias were quantified at different concentration levels (serum and plasma) over a 20-day period. Linearity was assessed covering a range from 4.08 µmol/L to 33.8 µmol/L. Limit of blank (LoB) and limit of detection (LoD) were established based on a total of 120 blank and low-level samples. The method comparison was based on 58 plasma samples. Within-run imprecision ranged from 0.7% to 1.2% and within-laboratory imprecision from 1.4% to 3.3%. Relative bias for the 2 serum pools with known target values was less than 2.5%. The assay did not deviate from linearity over the tested measuring range. LoB and LoD were 0.12 µmol/L and 0.23 µmol/L, respectively. The method comparison revealed an average deviation of 11.5% (2.016 µmol/L), and the linear regression fit was y = 1.464 + 0.795x. The LT-SYS copper assay characterized in this study showed a fully acceptable performance with good degrees of imprecision and bias, no deviation from linearity in the relevant measuring rangem, and very low LoB and LoD. © American Society for Clinical Pathology, 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

A novel zinc finger protein 219-like (ZNF219L) is involved in the regulation of collagen type 2 alpha 1a (col2a1a) gene expression in zebrafish notochord.

Science.gov (United States)

Lien, Huang-Wei; Yang, Chung-Hsiang; Cheng, Chia-Hsiung; Hung, Chin-Chun; Liao, Wei-Hao; Hwang, Pung-Pung; Han, Yu-San; Huang, Chang-Jen

2013-01-01

The notochord is required for body plan patterning in vertebrates, and defects in notochord development during embryogenesis can lead to diseases affecting the adult. It is therefore important to elucidate the gene regulatory mechanism underlying notochord formation. In this study, we cloned the zebrafish zinc finger 219-like (ZNF219L) based on mammalian ZNF219, which contains nine C2H2-type zinc finger domains. Through whole-mount in situ hybridization, we found that znf219L mRNA is mainly expressed in the zebrafish midbrain-hindbrain boundary, hindbrain, and notochord during development. The znf219L morpholino knockdown caused partial abnormal notochord phenotype and reduced expression of endogenous col2a1a in the notochord specifically. In addition, ZNF219L could recognize binding sites with GGGGG motifs and trigger augmented activity of the col2a1a promoter in a luciferase assay. Furthermore, in vitro binding experiments revealed that ZNF219L recognizes the GGGGG motifs in the promoter region of the zebrafish col2a1a gene through its sixth and ninth zinc finger domains. Taken together, our results reveal that ZNF219L is involved in regulating the expression of col2a1a in zebrafish notochord specifically.

Lipoxin A4 stable analogs reduce allergic airway responses via mechanisms distinct from CysLT1 receptor antagonism.

Science.gov (United States)

Levy, Bruce D; Lukacs, Nicholas W; Berlin, Aaron A; Schmidt, Birgitta; Guilford, William J; Serhan, Charles N; Parkinson, John F

2007-12-01

Cellular recruitment during inflammatory/immune responses is tightly regulated. The ability to dampen inflammation is imperative for prevention of chronic immune responses, as in asthma. Here we investigated the ability of lipoxin A4 (LXA4) stable analogs to regulate airway responses in two allergen-driven models of inflammation. A 15-epi-LXA4 analog (ATLa) and a 3-oxa-15-epi-LXA4 analog (ZK-994) prevented excessive eosinophil and T lymphocyte accumulation and activation after mice were sensitized and aerosol-challenged with ovalbumin. At 50% and to a greater extent than equivalent doses of the CysLT1 receptor antagonist montelukast. Distinct from montelukast, ATLa treatment led to marked reductions in cysteinyl leukotrienes, interleukin-4 (IL-4), and IL-10, and both ATLa and ZK-994 inhibited levels of IL-13. In cockroach allergen-induced airway responses, both intraperitoneal and oral administration of ZK-994 significantly reduced parameters of airway inflammation and hyper-responsiveness in a dose-dependent manner. ZK-994 also significantly changed the balance of Th1/Th2-specific cytokine levels. Thus, the ATLa/LXA4 analog actions are distinct from CysLT1 antagonism and potently block both allergic airway inflammation and hyper-reactivity. Moreover, these results demonstrate these analogs' therapeutic potential as new agonists for the resolution of inflammation.

O berço francês na nossa educação: do projeto do Colégio Pedro II ao Bac-ENEM

Directory of Open Access Journals (Sweden)

Cynthia Agra de Brito Neves

2015-12-01

Full Text Available Este artigo tem por finalidade refletir sobre as raízes e os vínculos transversais do Brasil com a França a partir da investigação das heranças e influências da pedagogia francesa na formação do nosso ensino secundário, sobretudo em meados do século XIX, quando o Colégio Pedro II, a escola modelo, era referência nacional e importava currículos e programas de estudos dos lycées franceses. Almeida (2000, Doria (1997, Santos (2003, Vechia e Cavazotti et al (2003 e, principalmente, Razzini (2000 foram contribuições preciosas para constatarmos o papel das ideias libertárias e literárias dessa pedagogia na excelência desse Colégio no período do Império à República no Brasil. Criado em 1837, sob tutela do Estado e com o nome do imperador, o Colégio estabelecia um projeto de nação baseado nessas ideias. A instituição – responsável pela formação de nomes como Joaquim Manuel de Macedo, Manuel Bandeira e Pedro Nava – contava com um corpo docente de prestígio que se dedicou, durante décadas, à formação dos filhos da elite brasileira que procurava naquela escola uma educação inspirada no modelo clássico e humanista francês.

Transcriptome analysis of skin fibroblasts with dominant negative COL3A1 mutations provides molecular insights into the etiopathology of vascular Ehlers-Danlos syndrome.

Science.gov (United States)

Chiarelli, Nicola; Carini, Giulia; Zoppi, Nicoletta; Ritelli, Marco; Colombi, Marina

2018-01-01

Vascular Ehlers-Danlos syndrome (vEDS) is a dominantly inherited connective tissue disorder caused by mutations in the COL3A1 gene that encodes type III collagen (COLLIII), which is the major expressed collagen in blood vessels and hollow organs. The majority of disease-causing variants in COL3A1 are glycine substitutions and in-frame splice mutations in the triple helix domain that through a dominant negative effect are associated with the severe clinical spectrum potentially lethal of vEDS, characterized by fragility of soft connective tissues with arterial and organ ruptures. To shed lights into molecular mechanisms underlying vEDS, we performed gene expression profiling in cultured skin fibroblasts from three patients with different structural COL3A1 mutations. Transcriptome analysis revealed significant changes in the expression levels of several genes involved in maintenance of cell redox and endoplasmic reticulum (ER) homeostasis, COLLs folding and extracellular matrix (ECM) organization, formation of the proteasome complex, and cell cycle regulation. Protein analyses showed that aberrant COLLIII expression is associated with the disassembly of many structural ECM constituents, such as fibrillins, EMILINs, and elastin, as well as with the reduction of the proteoglycans perlecan, decorin, and versican, all playing an important role in the vascular system. Furthermore, the altered distribution of the ER marker protein disulfide isomerase PDI and the strong reduction of the COLLs-modifying enzyme FKBP22 are consistent with the disturbance of ER-related homeostasis and COLLs biosynthesis and post-translational modifications, indicated by microarray analysis. Our findings add new insights into the pathophysiology of this severe vascular disorder, since they provide a picture of the gene expression changes in vEDS skin fibroblasts and highlight that dominant negative mutations in COL3A1 also affect post-translational modifications and deposition into the ECM of

Transcriptome analysis of skin fibroblasts with dominant negative COL3A1 mutations provides molecular insights into the etiopathology of vascular Ehlers-Danlos syndrome.

Directory of Open Access Journals (Sweden)

Nicola Chiarelli

Full Text Available Vascular Ehlers-Danlos syndrome (vEDS is a dominantly inherited connective tissue disorder caused by mutations in the COL3A1 gene that encodes type III collagen (COLLIII, which is the major expressed collagen in blood vessels and hollow organs. The majority of disease-causing variants in COL3A1 are glycine substitutions and in-frame splice mutations in the triple helix domain that through a dominant negative effect are associated with the severe clinical spectrum potentially lethal of vEDS, characterized by fragility of soft connective tissues with arterial and organ ruptures. To shed lights into molecular mechanisms underlying vEDS, we performed gene expression profiling in cultured skin fibroblasts from three patients with different structural COL3A1 mutations. Transcriptome analysis revealed significant changes in the expression levels of several genes involved in maintenance of cell redox and endoplasmic reticulum (ER homeostasis, COLLs folding and extracellular matrix (ECM organization, formation of the proteasome complex, and cell cycle regulation. Protein analyses showed that aberrant COLLIII expression is associated with the disassembly of many structural ECM constituents, such as fibrillins, EMILINs, and elastin, as well as with the reduction of the proteoglycans perlecan, decorin, and versican, all playing an important role in the vascular system. Furthermore, the altered distribution of the ER marker protein disulfide isomerase PDI and the strong reduction of the COLLs-modifying enzyme FKBP22 are consistent with the disturbance of ER-related homeostasis and COLLs biosynthesis and post-translational modifications, indicated by microarray analysis. Our findings add new insights into the pathophysiology of this severe vascular disorder, since they provide a picture of the gene expression changes in vEDS skin fibroblasts and highlight that dominant negative mutations in COL3A1 also affect post-translational modifications and deposition

Increased Frequency of ColV Plasmids and Mannose-Resistant Hemagglutinating Activity in an Escherichia coli K1 Population

OpenAIRE

1984-01-01

The expression of traits linked to pathogenicity was studied in a population of Escherichia coli K1 strains. It was found that E. coli K1 strains isolated from extraintestinal infection harbor the ColV plasmid and express mannose-resistant hemagglutinating activity type VI with a high frequency. The presence of these properties may play a role in the ability of some E. coli K1 serogroups to invade.

Compósito de colágeno com silicato e hidroxiapatita como material para endodontia: preparação e caracterização = Collagen composite with silicate and hydroxyapatite as endodontic material: preparation and characterization

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E'Gues, Miguel Antônio Menezes

2008-01-01

Full Text Available Objetivo: Este trabalho teve por objetivo preparar e caracterizar um novo compósito de colágeno com silicato e hidroxiapatita que possa vir a ser utilizado como material endodôntico em capeamento pulpar direto e em pulpotomia. Metodologia: Os materiais utilizados como matéria-prima foram colágeno do tipo I na forma aniônica, silicato (cimento Portland Branco e hidroxiapatita sintetizada. Estes materiais foram misturados em proporções variadas de modo a obter um material compósito na forma de pasta. Os materiais obtidos foram caracterizados por técnicas de calorimetria exploratória diferencial, termogravimetria, microscopia eletrônica de varredura (MEV, espectrometria de dispersão de energia de Raios-X e espectroscopia na região do infravermelho. Resultados: A presença de colágeno no compósito foi demonstrada por transições térmicas típicas daquelas encontradas para o colágeno do tipo I (~48ºC, independentemente da proporção utilizada, e também por imagens de MEV, onde as fibras colagênicas também puderam ser observadas interligando as partículas de silicato. A composição química de todos compósitos de colágeno foi praticamente idêntica à do silicato utilizado. Conclusão: Os resultados mostraram que a estrutura da matriz de colágeno nos compósitos testados não foi alterada. Esta é uma característica importante para a utilização em endodontia do material compósito proposto, objetivando uma reparação tecidual de forma "mais natural"

77 FR 64718 - Safety Zone; Steam Ship Col. James M. Schoonmaker Relocation Project, Maumee River, Toledo, OH

Science.gov (United States)

2012-10-23

...The Coast Guard is establishing a temporary Safety Zone on the waters of Maumee River, Toledo, Ohio, in support of the relocation of S/S Col. James M. Schoonmaker (hereinafter referred to as `the Schoonmaker'). This temporary safety zone will encompass all waters of the Maumee River within a 100 yard radius of the Schoonmaker as it makes its transit from International Park to Skyway Marina. This temporary Safety Zone is necessary to protect persons operating around the area.

Géis de colágeno aniônico: ransana como biomateriais. Preparação e caracterização físico-química

OpenAIRE

Goissis,Gilberto; Góes,Júlio C.

1997-01-01

Este trabalho descreve o efeito da ransana, um polissacarídeo bacteriano, sobre géis de colágeno aniônico. A interação colágeno:ransana ocorreu independentemente do pH, mesmo com baixas concentrações de ransana, e os materiais obtidos no estado sólido foram caracterizados por serem mais estáveis térmicamente à medida em que se aumenta a concentração do polissacarídeo. Nenhuma alteração na estrutura secundária em tripla hélice do tropocolágeno foi observada. O efeito mais significativo da rans...

Prácticas escolares en el Colégio Americano: un enfoque en la cultura material

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Marlucy do Socorro Aragão de Sousa

2017-05-01

Full Text Available Este artículo aborda las prácticas escolares y la cultura material de la escuela en Belém do Pará, a finales del siglo XIX. El objetivo es analizar cómo se configuraron la cultura material y las prácticas escolares en el Colégio Americano, ideado por José Veríssimo entre 1884 y 1890. Se usa como fuente la obra Noticia Geral sobre o Collegio Americano. Se utilizó la metodología histórico-hermenéutica. Al analizar la cultura material de la escuela, se tiene como horizonte la percepción de sus vínculos con el contexto histórico, cultural y social en el que se generó un nuevo modelo de educación que destacaba la civilidad, la nación y la moral. Por consiguiente, dichos artefactos no eran simples objetos, sino que revelaron las relaciones conflictivas o pacíficas de aquel contexto, e incluso las prácticas escolares de la institución. Se observa que el uso y la descripción de los objetos dispuestos en la estructura del Colégio Americano, para su creador, tenían el objetivo de establecer y mantener normas que debían formar hombres disciplinados, que se vincularan directamente con el propósito de la educación física, moral y cívica de los estudiantes.

Estandarización de un proceso de extracción de colágeno a partir de los residuos de fileteo de tilapia (Oreochromis sp) y cachama (Piaractus brachypomus) / Standardization of a process for extracting collagen from filleting waste from tilapia (Oreochromis sp) and cachama (Piaractus brachypomus)

OpenAIRE

Serrano Gaona, Jenifer Carolina

2011-01-01

Se estandarizó un proceso de extracción y purificación de colágeno ácido soluble a partir de pieles de tilapia (Oreochromis sp) y de cachama (Piaractus brachypomus) utilizando análisis estadísticos. Los resultados indicaron que el rendimiento de colágeno ácido soluble fue de 33,3% en base seca. El patrón de alto peso molecular utilizado para SDS – PAGE reveló que el colágeno obtenido de pieles de tilapia o de pieles de cachama es colágeno tipo I. La temperatura de desnaturalización (Td) del c...

Influence of aging on the quality of the skin of white women: the role of collagen Influência do envelhecimento na qualidade da pele de mulheres brancas: o papel do colágeno

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Eloina do Rocio Valenga Baroni

2012-10-01

Full Text Available PURPOSE: Evaluate the influence of aging on the quality of the skin of white women, analyzing the dermal collagen. METHODS: Pre-auricular flaps were collected for histological and morphometric analysis of 218 white women who underwent spontaneous facial aesthetic plastic surgery. Picrosirius ultrared stain was used for analysis and quantification of collagen in five age groups (OBJETIVO: Avaliar a influência do envelhecimento na qualidade da pele de mulheres brancas analisando o colágeno dérmico. MÉTODOS: Realizou-se análise histológica e morfométrica de 218 retalhos pré-auriculares de mulheres brancas que se submeteram espontaneamente à cirurgia estética facial. Foi usada a coloração de Picrosirius Ultrared para analisar e quantificar os colágenos I, III e total em cinco grupos etários (<40 anos, 40 a 49 anos, 50 a 59 anos, 60 a 69 anos e 70 a 79 anos. RESULTADOS: A análise histológica mostrou alterações sugestivas de envelhecimento cutâneo (fragmentação e desorganização das fibras de colágeno, especialmente em pacientes acima de 60 anos. Não houve diferenças significantes entre a idade e a espessura da derme e da epiderme, mas houve diferenças significantes entre as percentagens de colágeno I, III e total (p<0,001 com o aumento da idade. CONCLUSÃO: Existe redução do colágeno com o aumento da idade e um aumento na sua degradação, levando à fragmentação das fibras.

Bacillus sp. LT3 improves the survival of gnotobiotic brine shrimp (Artemia franciscana) larvae challenged with Vibrio campbellii by enhancing the innate immune response and by decreasing the activity of shrimp-associated vibrios.

Science.gov (United States)

Niu, Yufeng; Defoirdt, Tom; Baruah, Kartik; Van de Wiele, Tom; Dong, Shuanglin; Bossier, Peter

2014-10-10

Bacteria belonging to the genus Bacillus are amongst the most intensively studied group of bacteria for use as probiotics in aquaculture. However, the exact mechanism of action of these bacteria is often not well described, and the microbiota that are naturally present in cultures of test organisms often compromise the interpretation of the results. The present study aimed to evaluate the putative probiotic effect of Bacillus sp. LT3 in a model system with gnotobiotic brine shrimp Artemia franciscana larvae. The strain significantly increased the survival of brine shrimp larvae challenged with Vibrio campbellii when administered 6h before the challenge. Under these conditions, LT3 was able to colonize the brine shrimp gastrointestinal tract and to decrease the in vivo pathogen activity as indicated by the bioluminescence of the V. campbellii associated with brine shrimp larvae. In order to investigate the effect of the Bacillus strain on the innate immune system of the brine shrimp larvae, prophenoloxidase and transglutaminase mRNA levels were monitored, while heat shock protein 70 mRNA levels were measured as an indicator of physiological stress. Interestingly, 12h after challenge, the prophenoloxidase mRNA level in the larvae pre-treated with LT3 and challenged with V. campbellii was approximately 8-fold higher than in the other treatments. Further, a decreased mRNA level of transglutaminase gene and heat shock protein 70 gene suggested that pretreatment with LT3 results in less stress and tissue damage in the brine shrimp larvae upon V. campbellii challenge. These results indicated that Bacillus sp. LT3 could improve the survival of brine shrimp larvae when challenged with pathogenic V. campbellii, both by decreasing the in vivo activity of the pathogen and by priming the innate immune response through activating the prophenoloxidase system. Copyright © 2014 Elsevier B.V. All rights reserved.

Xarxes educatives 2.1. Mitjans socials, entorns col·laboratius i processos d'ensenyament i aprenentatge

OpenAIRE

Levis, Diego; Diéguez, Sol; Rey, Eugenia

2011-01-01

El projecte Xarxes Educatives 2.1 es proposa indagar les possibilitats que ofereixen els mitjans socials i els entorns col·laboratius a internet en els processos d'ensenyament i aprenentatge. Per a això, en cursos presencials i en cursos de capacitació docent de modalitat semipresencial fem servir una aplicació de la web 2.0 destinada a la creació de xarxes socials. L'aplicació es va configurar d'acord amb els objectius pedagògics de cada un dels cursos inclosos en el projecte. Es va establir...

An estimation of uncertainties in containment P/T analysis using CONTEMPT/LT code

International Nuclear Information System (INIS)

Kang, Y.M.; Park, G.C.; Lee, U.C.; Kang, C.S.

1991-01-01

In a nuclear power plant, the containment design pressure and temperature (P/T) have been established based on the unrealistic conservatism with suffering from a drawback in the economics. Thus, it is necessary that the uncertainties of design P/T values have to be well defined through an extensive uncertainty analysis with plant-specific input data and or models used in the computer code. This study is to estimate plant-specific uncertainties of containment design P/T using the Monte Carlo method in Kori-3 reactor. Kori-3 plant parameters and Uchida heat transfer coefficient are selected to be treated statistically after the sensitivity study. The Monte Carlo analysis has performed based on the response surface method with the CONTEMPT/LT code and Latin Hypercube sampling technique. Finally, the design values based on 95 %/95 % probability are compared with worst estimated values to assess the design margin. (author)

O hormônio de crescimento e a concentração de colágeno na cicatrização de feridas cutâneas de ratos

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Biondo-Simões Maria de Lourdes Pessole

2000-01-01

Full Text Available Investiga-se a interferência do hormônio de crescimento no processo de cicatrização cutânea de ratos. Utilizaram-se 50 ratos machos com 170 dias de idade divididos em 2 grupos: controle (n=25 e experimento (n=25. Submeteram-se os ratos dos dois grupos à laparotomia mediana de 4 centímetros sob anestesia inalatória de éter etílico. Realizou-se a laparorrafia com 2 planos de sutura, o primeiro peritônio-músculo-aponevrótico e o segundo o da pele com chuleio contínuo de fio monofilamentar de náilon 4.0. Os animais do grupo experimento receberam 0,4U/kg/dia de hormônio de crescimento por via subcutânea diluído em 0,12 ml de água destilada e os do grupo controle recebiam igual volume do veículo pela mesma via. Realizaram-se as aferições após 36, 72, 168, 240 e 336 horas, ressecando-se retalhos da parede abdominal contendo as cicatrizes, que após preparação histológica e tratamento pelo Sirius red, forneceram a concentração do colágeno. O percentual da área de cicatriz ocupada por colágeno foi maior no grupo experimento nas 36 horas (p=0,0106, 72 horas (p=0,0089, 168 horas (p=0,0149 e 336 horas (p=0,0116. O percentual de colágeno I foi semelhante nos dois grupos nas 36 e 72 horas e maior no grupo experimento nas 168 horas (p=0,0218, 240 horas (p=0,0067 e nas 336 horas (p=0,0027. O percentual de colágeno III apareceu em proporção semelhante nos 2 grupos nas 36 e 72 horas. Sua proporção foi maior no grupo controle nas 168 horas (p=0,0216, 240 horas (p=0,0067 e 336 horas (p=0,0027. Estes dados permitiram concluir que o hormônio de crescimento levou a maior concentração de colágeno e acelerou a maturação das cicatrizes.

Antalya-Korkuteli Yöresinde Üretilen Kültür Mantarlarının (Agaricus bisporus) Beslenme Durumlarının Belirlenmesi

OpenAIRE

SÖNMEZ, Sahriye; ÜRAS, Dilek Saadet; DEMİR, Erbil; ÖZEN, Nil; KILIÇ, Ezgi

2018-01-01

Kültür mantarcılığı; üretim odalarında sene boyunca üretim yapılabilmesi, diğer tarım kollarındaki gibi ekolojik şartlara bağlılığının olmaması ve uygun koşulların sağlanmasıyla yılda aynı alandan altı defa ürün alınabilmesi nedeniyle çok karlı bir tarımsal faaliyet alanıdır.Türkiye'de mantarcılık faaliyetlerinin yoğun olarak yürütüldüğü Antalya-Korkuteli yöresinde yetiştirilen kültür mantarlarının besin içeriklerinin araştırıldığı bu çalışmada; ilçede faaliyet gösteren 11 farklı işletme...

Interpretation of the CABRI LT1 test with SAS4A-code analysis

International Nuclear Information System (INIS)

Sato, Ikken; Onoda, Yu-uichi

2001-03-01

In the CABRI-FAST LT1 test, simulating a ULOF (Unprotected Loss of Flow) accident of LMFBR, pin failure took place rather early during the transient. No fuel melting is expected at this failure because the energy injection was too low and a rapid gas-release-like response leading to coolant-channel voiding was observed. This channel voiding was followed by a gradual fuel breakup and axial relocation. With an aid of SAS4A analysis, interpretation of this test was performed. Although the original SAS4A model was not well fitted to this type of early pin failure, the global behavior after the pin failure was reasonably simulated with temporary modifications. Through this study, gas release behavior from the failed fuel pin and its effect on further transient were well understood. It was also demonstrated that the SAS4A code has a potential to simulate the post-failure behavior initiated by a very early pin failure provided that necessary model modification is given. (author)

Análise das potencialidades e barreiras referentes ao desenvolvimento e à implementação do biodiesel a partir do óleo de palma na Colômbia

OpenAIRE

Ariel Augusto Amaya Avila

2009-01-01

A Colômbia, a partir de 2003, prevendo um déficit na sua produção de combustíveis e tentando reativar a economia rural, iniciou seu programa nacional de biocombustíveis apoiado em políticas, regulamentos e incentivos econômicos para o novo setor energético. É assim que esta dissertação tem como objetivo descrever e analisar o processo de implementação do programa de biodiesel na Colômbia a partir de óleo de palma, determinando a capacidade produtiva do país e discutindo as barreiras existente...

Images and Memories: photography as document and research source for the historical recovery of Colônia Esperança (1935-1963 Imagens e Memórias: a fotografia como documento e fonte de pesquisa para a recuperação histórica da Colônia Esperança (1935-1963

Directory of Open Access Journals (Sweden)

Larissa Ayumi Sato

2011-08-01

Full Text Available The use of photography as research source and historical document has been set as important instrument in the process of historical recovery of recent settlement places as Colônia Esperança, rural community located in the city of Arapongas, on the border of Apucarana. This study considers the rescue of this community’s ancient images and the search of details that can contextualize this pictures become important tools for the recovery of scenarios that nowadays are modified by the passage of the time. In the case of Colônia Esperança, that appeared as a redoubt of catholic Japanese people searching for more fertile lands and better life conditions, around a common ideology (keep their religious practices and the customs of their origin country, this kind of analysis become particularly relevant due to the history of this location is not properly systematized. The colony was founded in 1935, even before the city of Arapongas, that was started in 1937, product of colonization works made by Companhia de Terras Norte do Paraná. This sense, this research uses as methodological procedures the reading, the iconographic analysis and the iconological interpretation of photography’s elements, bibliographic and documentary research, in addition to the oral history – as technique – to salvage colonization and everyday life aspects in the initial decades (30’s to 60’s in the location. In the end of this project, it’s expected the obtained systematization about the history of Colônia Esperança, enriched with rare pictures that were in hand of the pioneer families, unpublished testimonials and documents, fundamental for this process, can contribute to evidence the role of the photography as historical document and research source. A utilização de fotografias como fonte de pesquisa e documento histórico tem se configurado como importante instrumento no processo de reconstituição da história de lugares de coloniza-ção recente, como a

Expressão citofotométrica do fator de proliferação celular ki-67 no bócio colóide e no carcinoma papilífero da tireóide

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Gleim Dias de Souza

Full Text Available OBJETIVO: Comparar a expressão citofotométrica quantitativa do fator de proliferação celular Ki-67 no bócio colóide com o do carcinoma papilífero da tireóide. MÉTODOS: Foram estudadas a expressão da proteína Ki-67, em 12 casos de bócio colóide da tireóide e 20 casos de carcinoma papilífero da tireóide. Os núcleos celulares imunomarcados foram quantificados através do software SAMBA 4000 ® e do software IMMUNO®, analisando o índice de marcagem e densidade óptica. Foi estimado o coeficiente de correlação de Spearmane e o teste não-paramétrico de Mann-Whitney. RESULTADOS: Foi rejeitada a hipótese nula para o índice de marcagem. confirmando que existe diferença significativa entre o bócio colóide e o carcinoma papilífero da tireóide, quanto ao índice de marcagem do Ki-67, que são maiores nos carcinomas papilíferos da tireóide. Não foi encontrada diferença quanto à densidade óptica. Quanto ao bócio colóide, o coeficiente de correlação estimado entre o índice de marcagem e a densidade óptica do Ki-67 foi igual a 0,78. No bócio colóide, houve associação positiva e significativa entre o índice de marcagem e a densidade ótica do Ki-67. Para o carcinoma papilífero da tireóide o coeficiente de correlação estimado entre o índice de marcagem e a densidade ótica do Ki-67 foi igual a 0,18. Não houve no carcinoma papilífero de tireóide, associação entre o índice de marcagem e a densidade ótica do Ki-67. CONCLUSÃO: A expressão citofotométrica do Ki67 no bócio colóide teve índice médio de marcação de 13,92% e densidade óptica média de 36,43; a expressão citofotométrica do Ki-67 no carcinoma papilífero teve índice médio de marcação de 38,29% e densidade óptica média de 48,07%; há maior proliferação celular no carcinoma papilífero em comparação com o bócio colóide na expressão do Ki-67.

Basic Fibroblast Growth Factor Fused with Tandem Collagen-Binding Domains from Clostridium histolyticum Collagenase ColG Increases Bone Formation

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Hiroyuki Sekiguchi

2018-01-01

Full Text Available Basic fibroblast growth factor 2 (bFGF accelerates bone formation during fracture healing. Because the efficacy of bFGF decreases rapidly following its diffusion from fracture sites, however, repeated dosing is required to ensure a sustained therapeutic effect. We previously developed a fusion protein comprising bFGF, a polycystic kidney disease domain (PKD; s2b, and collagen-binding domain (CBD; s3 sourced from the Clostridium histolyticum class II collagenase, ColH, and reported that the combination of this fusion protein with a collagen-like peptide, poly(Pro-Hyp-Gly10, induced mesenchymal cell proliferation and callus formation at fracture sites. In addition, C. histolyticum produces class I collagenase (ColG with tandem CBDs (s3a and s3b at the C-terminus. We therefore hypothesized that a bFGF fusion protein containing ColG-derived tandem CBDs (s3a and s3b would show enhanced collagen-binding activity, leading to improved bone formation. Here, we examined the binding affinity of four collagen anchors derived from the two clostridial collagenases to H-Gly-Pro-Arg-Gly-(Pro-Hyp-Gly12-NH2, a collagenous peptide, by surface plasmon resonance and found that tandem CBDs (s3a-s3b have the highest affinity for the collagenous peptide. We also constructed four fusion proteins consisting of bFGF and s3 (bFGF-s3, s2b-s3b (bFGF-s2b-s3, s3b (bFGF-s3b, and s3a-s3b (bFGF-s3a-s3b and compared their biological activities to those of a previous fusion construct (bFGF-s2b-s3 using a cell proliferation assay in vitro and a mouse femoral fracture model in vivo. Among these CB-bFGFs, bFGF-s3a-s3b showed the highest capacity to induce mesenchymal cell proliferation and callus formation in the mice fracture model. The poly(Pro-Hyp-Gly10/bFGF-s3a-s3b construct may therefore have the potential to promote bone formation in clinical settings.

Lethal chondrodysplasia in a family of Holstein cattle is associated with a de novo splice site variant of COL2A1

DEFF Research Database (Denmark)

Agerholm, Jørgen Steen; Menzi, Fiona; McEvoy, Fintan

2016-01-01

was predicted to affect splicing as it altered the conserved splice donor sequence GT at the 5’-end of COL2A1 intron 36, which was changed to AT. All five available cases carried the mutant allele in heterozygous state and all five dams were homozygous wild type. The sire VH Cadiz Captivo was shown...

Embryo genome profiling by single-cell sequencing for successful preimplantation genetic diagnosis in a family harboring COL4A1 c.1537G>A; p.G513S mutation

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Nayana H Patel

2016-01-01

Full Text Available CONTEXT: Genetic profiling of embryos (also known as preimplantation genetic diagnosis before implantation has dramatically enhanced the success quotient of in vitro fertilization (IVF in recent times. The technology helps in avoiding selective pregnancy termination since the baby is likely to be free of the disease under consideration. AIM: Screening of embryos free from c.1537G>A; p.G513S mutation within the COL4A1 gene for which the father was known in before be in heterozygous condition. SUBJECTS AND METHODS: Processing of trophectoderm biopsies was done from twelve embryos for c.1537G>A; p.G513S mutation within the COL4A1 gene. DNA extracted from isolated cells were subjected to whole genome amplification using an isothermal amplification and strand displacement technology. Oligonucleotide primers bracketing the mutation were synthesized and used to amplify 162 base pairs (bp polymerase chain reaction amplicons originating from each embryo which were subsequently sequenced to detect the presence or absence of the single base polymorphism. RESULTS: Three out of 12 embryos interrogated in this study were found to be normal while 9 were found to harbor the mutation in heterozygous condition. Implantation of one of the normal embryos following by chorionic villus sampling at 11 th week of pregnancy indicated that the baby was free from c.1537G>A; p.G513S mutation within the COL4A1 gene. CONCLUSIONS: Single-cell sequencing is a helpful tool for preimplantation embryo profiling. This is the first report from India describing the birth of a normal child through IVF procedure where a potential pathogenic COL4A1 allele was avoided using this technology.

Upregulated expression of integrin α1 in mesangial cells and integrin α3 and vimentin in podocytes of Col4a3-null (Alport mice.

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Brooke M Steenhard

Full Text Available Alport disease in humans, which usually results in proteinuria and kidney failure, is caused by mutations to the COL4A3, COL4A4, or COL4A5 genes, and absence of collagen α3α4α5(IV networks found in mature kidney glomerular basement membrane (GBM. The Alport mouse harbors a deletion of the Col4a3 gene, which also results in the lack of GBM collagen α3α4α5(IV. This animal model shares many features with human Alport patients, including the retention of collagen α1α2α1(IV in GBMs, effacement of podocyte foot processes, gradual loss of glomerular barrier properties, and progression to renal failure. To learn more about the pathogenesis of Alport disease, we undertook a discovery proteomics approach to identify proteins that were differentially expressed in glomeruli purified from Alport and wild-type mouse kidneys. Pairs of cy3- and cy5-labeled extracts from 5-week old Alport and wild-type glomeruli, respectively, underwent 2-dimensional difference gel electrophoresis. Differentially expressed proteins were digested with trypsin and prepared for mass spectrometry, peptide ion mapping/fingerprinting, and protein identification through database searching. The intermediate filament protein, vimentin, was upregulated ∼2.5 fold in Alport glomeruli compared to wild-type. Upregulation was confirmed by quantitative real time RT-PCR of isolated Alport glomeruli (5.4 fold over wild-type, and quantitative confocal immunofluorescence microscopy localized over-expressed vimentin specifically to Alport podocytes. We next hypothesized that increases in vimentin abundance might affect the basement membrane protein receptors, integrins, and screened Alport and wild-type glomeruli for expression of integrins likely to be the main receptors for GBM type IV collagen and laminin. Quantitative immunofluorescence showed an increase in integrin α1 expression in Alport mesangial cells and an increase in integrin α3 in Alport podocytes. We conclude that

Influence of tra genes of IncP and F plasmids on the mobilization of small Kanamycin resistance ColE1-Like plasmids in bacterial biofilms

Science.gov (United States)

Background: Horizontal gene transfer is a mechanism for movement of antibiotic resistance genes among bacteria. Some small kanamycin resistance (KanR) ColE1-like plasmids isolated from different serotypes of Salmonella enterica were shown to carry mobilization genes; although not self-transmissibl...

Detection of mutations in the COL4A5 gene by SSCP in X-linked Alport syndrome

DEFF Research Database (Denmark)

Hertz, Jens Michael; Juncker, I; Persson, U

2001-01-01

, three in-frame deletions, four nonsense mutations, and six splice site mutations. Twenty-two of the mutations have not previously been reported. Furthermore, we found one non-pathogenic amino acid substitution, one rare variant in a non-coding region, and one polymorphism with a heterozygosity of 28...... of type IV-collagen. We performed mutation analysis of the COL4A5 gene by PCR-SSCP analysis of each of the 51 exons with flanking intronic sequences in 81 patients suspected of X-linked Alport syndrome including 29 clear X-linked cases, 37 cases from families with a pedigree compatible with X...

DESARROLLO DE COMPETENCIAS EN LOS ESTUDIANTES DEL LABORATORIO DE MATERIALES DEL PROGRAMA DE INGENIERÍA MECÁNICA EN EL NÚCLEO LUZ-COL.

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Raysa Balza

2012-11-01

Full Text Available Esta investigación tiene como objetivo analizar las estrategias que contribuyen al desarrollo de competencias en los estudiantes del laboratorio de Materiales en el Programa de Ingeniería Mecánica Núcleo LUZ-COL. La investigación se catalogó como descriptiva, con un diseño de campo, no experimental, transeccional (Hernández y col., 2003. La población estuvo integrada or seis (6 docentes y 365 estudiantes. Seleccionando una muestra de 191 estudiantes, los otros elementos de la población fueron considerados en su totalidad. Se utilizaron como instrumentos una guía de observación y la entrevista estructurada. Evidenciando que el laboratorio presenta debilidades tanto en los aspectos infraestructurales como académicos; las estrategias utilizadas contribuyen medianamente a la formación de competencias en los estudiantes a través de un aprendizaje cooperativo. Asimismo, los equipos utilizados no cumplen con los aspectos tecnológicos y didácticos para el desarrollo de las prácticas.

Joint NASA/USAF Airborne Field Mill Program - Operation and safety considerations during flights of a Lear 28 airplane in adverse weather

Science.gov (United States)

Fisher, Bruce D.; Phillips, Michael R.; Maier, Launa M.

1992-01-01

A NASA Langley Research Center Learjet 28 research airplane was flown in various adverse weather conditions in the vicinity of the NASA Kennedy Space Center from 1990-1992 to measure airborne electric fields during the Joint NASA/USAF Airborne Field Mill Program. The objective of this program was to characterize the electrical activity in various weather phenomena common to the NASA-Kennedy area in order to refine Launch Commit Criteria for natural and triggered lightning. The purpose of the program was to safely relax the existing launch commit criteria, thereby increasing launch availability and reducing the chance for weather holds and delays. This paper discusses the operational conduct of the flight test, including environmental/safety considerations, aircraft instrumentation and modification, test limitations, flight procedures, and the procedures and responsibilities of the personnel in the ground station. Airborne field mill data were collected for all the Launch Commit Criteria during two summer and two winter deployments. These data are now being analyzed.

“Distributed hybrid” MH–CGH2 system for hydrogen storage and its supply to LT PEMFC power modules

Energy Technology Data Exchange (ETDEWEB)

Lototskyy, M., E-mail: mlototskyy@uwc.ac.za [HySA Systems Competence Centre, South African Institute for Advanced Materials Chemistry, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville 7535 (South Africa); Tolj, I.; Davids, M.W.; Bujlo, P. [HySA Systems Competence Centre, South African Institute for Advanced Materials Chemistry, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville 7535 (South Africa); Smith, F. [Impala Platinum Ltd, Springs (South Africa); Pollet, B.G. [HySA Systems Competence Centre, South African Institute for Advanced Materials Chemistry, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville 7535 (South Africa)

2015-10-05

Highlights: • Prototype hydrogen storage and supply system for LTPEMFC applications was developed. • Combination of MH and CGH2 tanks with common gas manifold was used. • Thermal coupling of fuel cell stack and MH tank was applied. • The system uses AB2-type MH; H2 equilibrium pressure ∼10 bar at room temperature. • Shorter H2 charge time and stable H2 supply at a fluctuating load were observed. - Abstract: This paper describes the layout and presents the results of the testing of a novel prototype “distributed hybrid” hydrogen storage and supply system that has the potential to be used for Low Temperature Proton Exchange Membrane Fuel Cell (LT-PEMFC) applications. The system consists of individual Metal Hydride (MH) and Compressed Gas (CGH2) tanks with common gas manifold, and a thermal management system where heat exchanger of the liquid heated-cooled MH tank is integrated with the cooling system of the LT-PEMFC BoP. The MH tank is filled with a medium-stability AB{sub 2}-type MH material (H{sub 2} equilibrium pressure of about 10 bar at room temperature). This innovative solution allows for (i) an increase in hydrogen storage capacity of the whole gas storage system and the reduction of H{sub 2} charge pressure; (ii) shorter charging times in the refuelling mode and smoother peaks of H{sub 2} consumption during its supply to the fuel cell stack; (iii) the use of standard parts with simple layout and lower costs; and (iv) adding flexibility in the layout and placement of the components of the hydrogen storage and supply system.

“Distributed hybrid” MH–CGH2 system for hydrogen storage and its supply to LT PEMFC power modules

International Nuclear Information System (INIS)

Lototskyy, M.; Tolj, I.; Davids, M.W.; Bujlo, P.; Smith, F.; Pollet, B.G.

2015-01-01

Highlights: • Prototype hydrogen storage and supply system for LTPEMFC applications was developed. • Combination of MH and CGH2 tanks with common gas manifold was used. • Thermal coupling of fuel cell stack and MH tank was applied. • The system uses AB2-type MH; H2 equilibrium pressure ∼10 bar at room temperature. • Shorter H2 charge time and stable H2 supply at a fluctuating load were observed. - Abstract: This paper describes the layout and presents the results of the testing of a novel prototype “distributed hybrid” hydrogen storage and supply system that has the potential to be used for Low Temperature Proton Exchange Membrane Fuel Cell (LT-PEMFC) applications. The system consists of individual Metal Hydride (MH) and Compressed Gas (CGH2) tanks with common gas manifold, and a thermal management system where heat exchanger of the liquid heated-cooled MH tank is integrated with the cooling system of the LT-PEMFC BoP. The MH tank is filled with a medium-stability AB 2 -type MH material (H 2 equilibrium pressure of about 10 bar at room temperature). This innovative solution allows for (i) an increase in hydrogen storage capacity of the whole gas storage system and the reduction of H 2 charge pressure; (ii) shorter charging times in the refuelling mode and smoother peaks of H 2 consumption during its supply to the fuel cell stack; (iii) the use of standard parts with simple layout and lower costs; and (iv) adding flexibility in the layout and placement of the components of the hydrogen storage and supply system

Somatic mosaicism for the COL7A1 mutation p.Gly2034Arg in the unaffected mother of a patient with dystrophic epidermolysis bullosa pruriginosa

NARCIS (Netherlands)

van den Akker, P. C.; Pasmooij, A. M. G.; Meijer, R.; Scheffer, H.; Jonkman, M. F.

Dystrophic epidermolysis bullosa (DEB) is a heritable blistering disorder caused by mutations in the type VII collagen gene, COL7A1. Although revertant mosaicism is well known in DEB, 'forward' somatic mosaicism, in which a pathogenic mutation arises on a wild-type (WT) background, extending beyond

Somatic mosaicism for the COL7A1 mutation p.Gly2034Arg in the unaffected mother of a patient with dystrophic epidermolysis bullosa pruriginosa

NARCIS (Netherlands)

Akker, P.C. van den; Pasmooij, A.M.; Meijer, R.; Scheffer, H.; Jonkman, M.F.

2015-01-01

Dystrophic epidermolysis bullosa (DEB) is a heritable blistering disorder caused by mutations in the type VII collagen gene, COL7A1. Although revertant mosaicism is well known in DEB, 'forward' somatic mosaicism, in which a pathogenic mutation arises on a wild-type (WT) background, extending beyond

Metais pesados, agrotóxicos e combustíveis: efeito na população de colêmbolos no solo Heavy metal, pesticides and fuels: effect in the population of collembola in the soil

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Zaida Inês Antoniolli

2013-06-01

Full Text Available O objetivo deste trabalho foi avaliar o desenvolvimento de colêmbolos no solo com diferentes níveis de metais pesados, combustíveis e agrotóxicos em condição de laboratório. Os tratamentos foram: Cádmio (1, 10 e 100mg kg-1 de solo; Cobre (50, 500 e 5000mg kg-1 de solo; Zinco (100, 1000 e 10000mg kg-1 de solo; fungicida epoxiconazol + piraclostrobina (1 e 2L ha-1; fungicida epoxiconazol (0,75 e 1,5L ha-1; herbicida glifosato (2 e 4L ha-1; óleo lubrificante queimado (5, 50 e 100mL kg-1 solo e óleo diesel (5, 50 e 100mL kg-1 solo e controle. Na dose de 1mg kg-1 de Cd no solo, houve grande aumento na população de colêmbolos e o pH do solo diminuiu à medida que aumentaram as doses de Cd, Zn e Cu no solo. Na presença de combustíveis, os colêmbolos apresentaram incapacidade de reprodução, independente da dose aplicada no solo. O número de colêmbolos apresentou incremento com o aumento da dosagem de glifosato e epoxiconazol. Os metais pesados Cu e Zn têm ação negativa sobre a população de colêmbolos e somente o Cd (1mg kg-1 solo proporciona aumento na população de colêmbolos no solo. A presença de óleo lubrificante queimado e óleo diesel no solo inibem o desenvolvimento dos colêmbolos no solo. Os agrotóxicos glifosato, epoxiconazol e epoxiconazol + piraclostrobina não influenciam negativamente sobre a população de colêmbolos no solo.The aim of this research was to evaluate the collembola development in soils with different levels of heavy metals, fuels and pesticides, in laboratory conditions. Treatments were: Cadmium - 1, 10 and 100mg kg-1 soil; Copper - 50, 500 and 5000mg kg-1 soil; Zinc - 100, 1000 and 10000 mg kg-1 soil; fungicide epoxiconazol + piraclostrobina - 1 and 2L ha-1; fungicide epoxiconazol - 0,75 and 1,5L ha-1; herbicide glyphosate - 2 and 4L ha-1; burned lubricating oil - 5, 50 and 100mL kg-1 soil and diesel oil - 5, 50 and 100mL kg-1 soil and control. In the 1mg kg-1 dose of Cd there was a great

Leukotriene D4 induces chemotaxis in human eosinophilc cell line, EoL-1 cells via CysLT1 receptor activation.

Science.gov (United States)

Shirasaki, Hideaki; Kanaizumi, Etsuko; Himi, Tetsuo

2017-11-01

Numerous reports have shown that cysteinyl leukotrienes (CysLTs) contribute to tissue accumulation of eosinophils in allergic airway inflammation. To date, only a few studies have reported that CysLTs promote chemotactic activity of human eosinophils in vitro. The purpose of this study was to investigate whether CysLTs promote chemotaxis in the human eosinophilic cell line, EoL-1. EoL-1 cells were induced to differentiate into mature eosinophil-like cells via incubation with butyric acid and cytokines (IL-3, IL-5 and GM-CSF). The chemotactic activity of the differentiated EoL-1 cells was assessed using the commercial cell migration assay kit. LTD 4 elicited dose-related chemotactic activity in the differntiated EoL-1 cells in the range of 1-100 nM. A typical bell-shaped dose-response curve was observed with optimal activity at 10 nM. The chemotactic activity elicited by LTD 4 (10 nM) was significantly inhibited by montelukast (control, 345 ± 19.2 × 10 3 RFU; LTD 4 10 nM alone, 511 ± 39.2 × 10 3 RFU; LTD 4 10 nM plus montelukast 100 nM, 387 ± 28.2 × 10 3 RFU). LTD 4 induces migration in eosinophilic cells via activation of CysLT1 receptor. The present in vitro model may be useful for elucidation of the mechanism underlying CysLT-induced tissue eosinophilia.

An atlas of L-T transition brown dwarfs with VLT/XShooter

Science.gov (United States)

Marocco, F.; Day-Jones, A. C.; Jones, H. R. A.; Pinfield, D. J.

In this contribution we present the first results from a large observing campaign we are carrying out using VLT/Xshooter to obtain spectra of a large sample (˜250 objects) of L-T transition brown dwarfs. Here we report the results based on the first ˜120 spectra already obtained. The large sample, and the wide spectral coverage (300-2480 nm) given by Xshooter, will allow us to do a new powerful analysis, at an unprecedent level. By fitting the absorption lines of a given element (e.g. Na) at different wavelengths we can test ultracool atmospheric models and draw for the first time a 3D picture of stellar atmospheres at temperatures down to 1000K. Determining the atmospheric parameters (e.g. temperature, surface gravity and metallicity) of a big sample of brown dwarfs, will allow us to understand the role of these parameters on the formation of their spectra. The large number of objects in our sample also will allow us to do a statistical significant test of the birth rate and initial mass function predictions for brown dwarfs. Determining the shape of the initial mass function for very low mass objects is a fundamental task to improve galaxy models, as recent studies tep{2010Natur.468..940V} have shown that low-mass objects dominate in massive elliptical galaxies.

Diseño de un relleno sanitario manual para el recinto "Cristóbal Colón" - provincia de Esmeraldas

OpenAIRE

Ruiz Ortega, Jennifer Carolina; Unapanta Albán, Victoria Carolina

2015-01-01

This project, contains the design of a manual sanitary landfill for the recinto Cristóbal Colón, for which it was necessary to know the current situation, with regard to biotic, physical and socio-economic environment. First, is selected representative sample of the population, distributed among households and commercial establishments, in order to collect their waste for eight days and characterized by the quartering method. Once established the optimal way for the design of the landfi...

Esnek ve Rijit Yol Üst Yapılarında Gürültü Seviyelerinin Eş Zamanlı Ölçümü ve Analizi

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Hakan Yıldırım

2014-06-01

Full Text Available Beton yollar dünyada yaygın olarak kullanılmasına karşın ülkemizde gelişim gösterememiş, bu nedenle de genel olarak bitümlü sıcak karışım asfalt yollar tercih edilmiştir.Bu çalışmada, Türkiye'de yaygın olarak kullanılan bitümlü sıcak karışım asfalt yollar ve henüz yaygınlaşmamış olan beton yollar arasında gürültü seviyeleri yönünden bir karşılaştırma yapılmıştır. Bunun için Afyonkarahisar-Emirdağ arasında inşa edilmiş 2 km'lik beton yol ile bu yolun devamı olan bitümlü sıcak karışım asfalt yol arasındaki geçiş noktası çalışma koridoru olarak kullanılmıştır.Bu koridordaki beton yol ve bitümlü sıcak karışım asfalt yolun kenarlarına, ses girişimlerini en aza indirecek şekilde, ayrı ayrı gürültü ölçüm cihazı ve kamera düzenekleri yerleştirilmiş, yol yapım malzemesine bağlı olarak değişen gürültü seviyeleri eş zamanlı olarak kaydedilmiş, bulunan değerler analiz edilerek kaplama türleri ile gürültü seviyeleri arasındaki ilişki araştırılmıştır. Bu aşamada, kurulan düzenekler arasındaki mesafe trafik yoğunluğunun ve akış hızının değişimini engellemek için mümkün olduğunca kısa tutulmuştur.Yapılan ölçüm ve analizler sonunda beton yolların bitümlü sıcak karışım asfalt yollara göre ortalama gürültü seviyesinin aynı taşıt kompozisyonu ve trafik hacmi için yaklaşık 4 dB(A daha düşük olduğu ortaya konmuş ve ülkemizde de beton yolların gelişim göstermesi gerektiği vurgulanmıştır.

A criação do Colégio de Pedro II e seu impacto na constituição do magistério público secundário no Brasil

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Ana Waleska P. C. Mendonça

2013-12-01

Full Text Available Este trabalho se vincula a uma pesquisa mais ampla que tem como objeto de estudo o processo de construção de uma identidade profissional específica por parte de um segmento da categoria docente - o magistério público secundário - no Brasil, constituindo-se como um primeiro produto coletivo do grupo de investigação. A referida pesquisa parte de um recorte institucional e toma como alvo de estudo o Colégio Pedro II, que foi criado pelo governo imperial em 1837 e que, após a República, é mantido pelo governo federal, conservando, até meados do século XX, o caráter de instituição modelar para todo o ensino secundário brasileiro. O texto está dividido em duas partes. Na primeira, propõe-se a identificar o programa institucional que informou a criação do Colégio de Pedro II. Na segunda, desenvolve um estudo preliminar sobre a primeira geração de professores do Colégio, nomeados entre 1838 e 1847, buscando estabelecer uma caracterização geral: as formas de recrutamento; a distribuição pela diferentes disciplinas; as categorias docentes previstas nos estatutos de 1838; as trajetórias internas no Colégio, inclusive o tempo de docência na instituição; a formação acadêmica; a experiência docente anterior; as outras instituições educacionais/culturais em que atuavam, entre outros aspectos. As principais referências teóricas são os trabalhos de dois sociólogos franceses que se debruçaram sobre o processo de construção da identidade profissional docente, a saber: François Dubet e Claude Dubar.

The effect of 6-week high intensity interval training on the VEGF/COL-18 ratio and some echocardiographic indices in rats with myocardial infarction

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Sara Karbalaeifar

2016-12-01

Full Text Available Introduction: Myocardial infarction (MI is the irreversible cell death caused by ischemia in parts of myocardium. The molecular process of increased capillary density in response to activity and its appropriate intensity is not clear yet. Therefore, this research aimed to evaluate the effect of 6-week high intensity interval training on the VEGF/COL-18 ratio and echocardiographic indices in rats with MI. Methods: Twelve Wistar male rats of 10 weeks old and mean weight 250-300gr were allocated to two groups of experimental (60 minutes of interval treadmill running for four minutes with the intensity of 85-90 and two minutes of active rest at 50-60 percent of VO2max for four days a week for 6 weeks and control group (without any training. Real-time PCR was used to assess the expression of VEGF and COL-18 genes after inducing MI, and shortening fractional and ejection fraction were investigated as echocardiographic indices. Data were analyzed in SPSS18 using independent t test (α≤ 0.05. Results: The findings showed that there was no significant increase in the VEGF/COL-18 ratio in the HIIT group (1.856 mg/ml as compared with the control group (1.245 mg/ml (p=0.263. A significant increase was observed in the HIIT group for SF (77.461±7.022% and EF (41.625±6.847% as compared with the control group (64.483±3.695% and (31.320±3.460%, respectively (p=0.001. Conclusion: In general, 6 weeks of high intensity interval training can effectively increase angiogenesis factors and improve myocardial function in male Wistar rats after MI.

Xarxa interclase: un projecte col·laboratiu mitjançant tecnologies digitals

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Françoise Olmo Cazevieille

2015-12-01

Full Text Available Focalitzem aquest treball en l'aportació de les tecnologies de la informació i la comunicació (TIC a la pràctica i el desenvolupament d'una llengua estrangera i en els avantatges que ofereixen per a la creació d'activitats basades en la col·laboració i la interacció. Sense aquestes, tant els treballs en equip amb nadius com la cerca d'informació sobre temes especialitzats en la llengua estudiada serien complexos de dur a terme. En aquest article presentem el procediment seguit en un projecte didàctic, Xarxa interclasse, entre dues assignatures basades en l'aprenentatge de llengües estrangeres per a fins específics en un mateix campus universitari.?Es pretén, d'una banda, acostar l'educació superior a les exigències de la societat actual i, d'altra banda, aconseguir una docència motivadora i una participació activa dels discents on la tecnologia s'integra com a element pedagògic.

Next-generation sequencing and a novel COL3A1 mutation associated with vascular Ehlers-Danlos syndrome with severe intestinal involvement: a case report.

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Cortini, Francesca; Marinelli, Barbara; Seia, Manuela; De Giorgio, Barbara; Pesatori, Angela Cecilia; Montano, Nicola; Bassotti, Alessandra

2016-10-31

The vascular type of Ehlers-Danlos syndrome is an autosomal dominant connective tissue disorder caused by a mutation in the COL3A1 gene encoding pro-alpha1 chain of type III collagen. The vascular type of Ehlers-Danlos syndrome causes severe fragility of connective tissues with arterial and intestinal ruptures and complications in surgical and radiological treatments. We present a case of a 38-year-old Italian woman who was diagnosed as having the vascular type of Ehlers-Danlos syndrome. Genetic testing, conducted by Target Enrichment approach (Agilent Technologies), identified a new mutation c.1493G>A, p.G498D in exon 21 of COL3A1 gene (heterozygous state). This mutation disrupts the normal glycine-X-Y repetitions of type III procollagen by converting glycine to aspartic acid. We report a new genetic mutation associated with the vascular type of Ehlers-Danlos syndrome. We also describe clinical and genetic findings that are important to understand the genotype/phenotype correlation in patients with the vascular type of Ehlers-Danlos syndrome.

Distribuciones simuladas de valores de d13c de colágeno humano: implicancias para los estudios paleodietarios

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Barrientos, Gustavo

2005-01-01

Full Text Available El objetivo de este trabajo es presentar los primeros resultados derivados de la aplicación de un programa de simulación de distribuciones de valores de δ13C de colágeno humano (DeltaCarb130.0, desarrollado por los autores. DeltaCarb130.0 es un programa simple que simula la composición isotópica (δ13C del colágeno de huesos humanos obtenida a través de una dieta de composición variable, constituida por tres ingestas diarias, durante un período de 1825 días (5 años. La composición de la dieta y los valores de δ13C de cada recurso dietario (vegetales C3 y C4, carne de herbívoros consumidores de C3 y C4, de mamíferos marinos, de peces y moluscos se obtienen, en cada etapa de la simulación, a través de un proceso aleatorio a partir de datos de abundancia relativa y rangos de valores de δ13C de cada tipo de recurso. Se analizarán estadísticamente las distribuciones de valores de δ13C obtenidas a partir de n corridas del programa para cada combinación dietaria, con la finalidad de obtener medidas de tendencia central y de dispersión asociadas a cada tipo de dieta y calcular la probabilidad de obtener valores "outliers", un rasgo frecuente en el estudio de casos arqueológicos.

CysLT1 receptor-induced human airway smooth muscle cells proliferation requires ROS generation, EGF receptor transactivation and ERK1/2 phosphorylation

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Capra Valérie

2006-03-01

Full Text Available Abstract Background Cysteine-containing leukotrienes (cysteinyl-LTs are pivotal inflammatory mediators that play important roles in the pathophysiology of asthma, allergic rhinitis, and other inflammatory conditions. In particular, cysteinyl-LTs exert a variety of effects with relevance to the aetiology of asthma such as smooth muscle contraction, eosinophil recruitment, increased microvascular permeability, enhanced mucus secretion and decreased mucus transport and, finally, airway smooth muscle cells (ASMC proliferation. We used human ASMC (HASMC to identify the signal transduction pathway(s of the leukotriene D4 (LTD4-induced DNA synthesis. Methods Proliferation of primary HASMC was measured by [3H]thymidine incorporation. Phosphorylation of EGF receptor (EGF-R and ERK1/2 was assessed with a polyclonal anti-EGF-R or anti-phosphoERKl/2 monoclonal antibody. A Ras pull-down assay kit was used to evaluate Ras activation. The production of reactive oxygen species (ROS was estimated by measuring dichlorodihydrofluorescein (DCF oxidation. Results We demonstrate that in HASMC LTD4-stimulated thymidine incorporation and potentiation of EGF-induced mitogenic signaling mostly depends upon EGF-R transactivation through the stimulation of CysLT1-R. Accordingly, we found that LTD4 stimulation was able to trigger the increase of Ras-GTP and, in turn, to activate ERK1/2. We show here that EGF-R transactivation was sensitive to pertussis toxin (PTX and phosphoinositide 3-kinase (PI3K inhibitors and that it occurred independently from Src activity, despite the observation of a strong impairment of LTD4-induced DNA synthesis following Src inhibition. More interestingly, CysLT1-R stimulation increased the production of ROS and N-acetylcysteine (NAC abolished LTD4-induced EGF-R phosphorylation and thymidine incorporation. Conclusion Collectively, our data demonstrate that in HASMC LTD4 stimulation of a Gi/o coupled CysLT1-R triggers the transactivation of the EGF

Enterotoxigenic Escherichia coli Adhesin-Toxoid Multiepitope Fusion Antigen CFA/I/II/IV-3xSTaN12S-mnLTG192G/L211A-Derived Antibodies Inhibit Adherence of Seven Adhesins, Neutralize Enterotoxicity of LT and STa Toxins, and Protect Piglets against Diarrhea.

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Nandre, Rahul; Ruan, Xiaosai; Lu, Ti; Duan, Qiangde; Sack, David; Zhang, Weiping

2018-03-01

Enterotoxigenic Escherichia coli (ETEC) strains are a leading cause of children's diarrhea and travelers' diarrhea. Vaccines inducing antibodies to broadly inhibit bacterial adherence and to neutralize toxin enterotoxicity are expected to be effective against ETEC-associated diarrhea. 6×His-tagged adhesin-toxoid fusion proteins were shown to induce neutralizing antibodies to several adhesins and LT and STa toxins (X. Ruan, D. A. Sack, W. Zhang, PLoS One 10:e0121623, 2015, https://doi.org/10.1371/journal.pone.0121623). However, antibodies derived from His-tagged CFA/I/II/IV-2xSTa A14Q -dmLT or CFA/I/II/IV-2xSTa N12S -dmLT protein were less effective in neutralizing STa enterotoxicity and were not evaluated in vivo for efficacy against ETEC diarrhea. Additionally, His-tagged proteins are considered less desirable for human vaccines. In this study, we produced a tagless adhesin-toxoid MEFA (multiepitope fusion antigen) protein, enhanced anti-STa immunogenicity by including a third copy of STa toxoid STa N12S , and examined antigen immunogenicity in a murine model. Moreover, we immunized pregnant pigs with the tagless adhesin-toxoid MEFA protein and evaluated passive antibody protection against STa + or LT + ETEC infection in a pig challenge model. Results showed that tagless adhesin-toxoid MEFA CFA/I/II/IV-3xSTa N12S -mnLT R192G/L211A induced broad antiadhesin and antitoxin antibody responses in the intraperitoneally immunized mice and the intramuscularly immunized pigs. Mouse and pig serum antibodies significantly inhibited adherence of seven colonization factor antigen (CFA) adhesins (CFA/I and CS1 to CS6) and effectively neutralized both toxins. More importantly, suckling piglets born to the immunized mothers acquired antibodies and were protected against STa + ETEC and LT + ETEC diarrhea. These results indicated that tagless CFA/I/II/IV-3xSTa N12S -mnLT R192G/L211A induced broadly protective antiadhesin and antitoxin antibodies and demonstrate that this adhesin