Espacios dramáticos en Rojas Zorrilla

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Suárez Miramón, Ana

2007-06-01

Full Text Available Space is a fundamental element in theatre and Rojas uses both open and closed spaces with a great ability. He even alternates one and other, open and closed spaces, and includes light and darkness’ contrast in order to intensify the dramatic tension. A detailed analysis of the function of space in Lo que quería ver el marqués de Villena and Casarse por vengarse, shows the important role that space plays in the development of action, in the transmission of emotional feelings or dramatic situations, and even in the representation of theatrical features.El espacio resulta fundamental en el teatro y Rojas utiliza con gran habilidad tanto los espacios abiertos como los cerrados. Incluso alterna unos y otros y los acompaña del contraste entre luz y oscuridad para intensificar la tensión dramática. Un examen detenido de la función del espacio en Lo que quería ver el marqués de Villena y Casarse por vengarse, muestra la importancia del espacio para la acción, para transmitir estados emocionales, situaciones dramáticas e incluso representar la teatralidad.

Efecto de la maca roja (Lepidium meyenii sobre los niveles de IFN-γ en ratas ovariectomizadas

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Johanna Leiva-Revilla

Full Text Available Objetivos. Comparar el efecto de diferentes dosis de maca roja sobre los niveles de interferón gamma (IFN-γ en ratas ovariectomizadas (OVX. Materiales y métodos. Ratas hembras adultas fueron divididas al azar en los siguientes seis grupos: Grupo 1: ratas pesudo-ovariectomizadas (PO; Grupo 2: ratas OVX; Grupo 3: ratas OVX tratadas con 4 ug/kg de estradiol, y Grupo 4, 5 y 6: ratas OVX tratadas con extractos de maca con 2,15, 4,3 y 8,6 mg polifenoles/kilogramo de peso corporal, respectivamente. Resultados. Las ratas OVX mostraron niveles bajos de IFN-γ en comparación con las ratas PO. El estradiol y la maca roja revirtieron el efecto de la ovariectomía sobre los niveles de IFN-γ. La maca roja presenta un incremento dosis-respuesta de los niveles de IFN-γ (r=0,57, p<0,05. Conclusiones. La administración de la maca roja incrementa los niveles de IFN-γ en ratas ovariectomizadas.

A la memoria de José Antonio Rojas Tercero, 1959-2008

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José Miguel Fernández Fernández

2009-06-01

Full Text Available La revista recuerda la figura de José Antonio Rojas, Catedrático de Economía Financiera y Contabilidad dde la Universidad de Castilla-La Mancha desde el punto de vista humani y profesional

Aplasia pura de serie roja post-trasplante alogeneico de células progenitoras hematopoyeticas ABO incompatible

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E. Bulliorsky

2002-12-01

Full Text Available El trasplante alogeneico de células progenitoras hematopoyéticas (TCPH con incompatibilidad ABO entre el donante y el receptor puede en ocasiones asociarse a trastornos en la progenie eritroide desarrollada a partir de la médula ósea trasplantada, caracterizado por un funcionamiento tardío, inadecuado e incompleto de la misma. En este contexto, la aplasia pura de serie roja es la complicación más severa. Se han intentado tratamientos para la aplasia pura de serie roja post-TCPH con eritropoyetina o plasmaféresis, con relativo éxito. Algunos autores han informado también la utilización de globulina antilinfocitaria, asumiendo que dicha aplasia selectiva de la serie roja en la médula ósea trasplantada es mediada por un mecanismo inmune. En este trabajo se describe un paciente portador de una leucemia aguda en quien se realizó un TCPH alogeneico (ABO incompatible con su donante. Teniendo niveles bajos de aglutininas contra el grupo sanguíneo de la donante, desarrolló una aplasia pura de serie roja post - TCPH. La misma no mejoró con tratamiento con eritropoyetina o con un refuerzo de progenitores hematopoyéticos de sangre periférica de la misma donante (boost, resolviéndose totalmente luego de un tratamiento exitoso con globulina antilinfocitaria de origen equino.

Rojas Zorrilla ante la crítica romántica

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Pedraza Jiménez, Felipe B

2007-06-01

Full Text Available To Romantic Criticism, Rojas Zorrilla’s fame becomed obscure by the figure of his contemporary, friend and collaborator, Pedro Calderón de la Barca. This logical situation is clearly evident in the great german critics: Rojas Zorrilla is scarcely mentioned by the Schlegel brothers. To other historians he occupied a secondary place and the only exception was Johann Ludwig Tieck, who exalted his works even above Calderón masterpieces. It can be affirmed that Rojas Zorrilla reception was irregular. If we examine Musso Valiente’s library, Agustín Durán’s Discurso, and the romantic period editions, we will find some interest in his figure and that it was situated in a secondary place in relation to our great theater. It seems clear that Martínez de la Rosa and Alberto Lista were the critics who pay more attention to him. Martínez de la Rosa established an approach based in the neoclassic topics about our writer, meanwhile Alberto Lista developed a closer and original reading, specially centered in the poet’s tragic dimension and elements that, from his point of view, anticipated romantic Aesthetics. Rojas Zorrilla inclusion in romantic Literary Histories, and his edition in the «Biblioteca de Autores Españoles», prepared by Mesonero Romanos, make him enter the canon among the «six great stars» in Spanish Golden Age Theater.Para la crítica romántica, la fama de Rojas Zorrilla quedó oscurecida, de forma perfectamente lógica, por la de su coetáneo, amigo y colaborador don Pedro Calderón de la Barca. Esta situación es muy patente en los grandes críticos alemanes. Los hermanos Schlegel apenas citan a nuestro dramaturgo. Otros historiadores le conceden un lugar secundario, con la excepción de Johann Ludwig Tieck, que lo exaltó incluso por encima del autor de La vida es sueño. La recepción de su obra en España fue irregular. Del análisis de la biblioteca y escritos de Musso Valiente, del discurso de Agustín Durán, de las

Establecimiento y micropropagación de selenicereus megalanthus (pitahaya amarilla) e hylocereus polyrhizus (pitahaya roja)

OpenAIRE

Suarez Román, Rocío Stella; Caetano, Creuci María; Ramírez, Hernando

2014-01-01

Pitahaya amarilla y roja se establecieron y micropropagaron en condiciones in vitro vía organogénesis somática, a partir de hojas cotiledonares y fragmentos de cladodio  provenientes de plántulas con 20 días de desarrollo expuestos a medio Murashige & Skoog (MS), suplementado con auxinas (ANA) y citocininas (Kinetina y BAP). En el medio de cultivo M3 (BAP + Kinetina 2 mg/l) se obtuvieron entre cuatro y 11 brotes enraizados de 4 cm de longitud, en un periodo de 30 días. En  pitahaya roja s...

De lo inmaterial literario al monumento arquitectónico: la casa museo de Ricardo Rojas / From the literary immateriality to the architectural monument: the House Museum of Ricardo Rojas

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Amanda Salvioni

2015-02-01

The House Museum of Ricardo Rojas, built in 1927 by the architect Angel Guido in Buenos Aires, is an ideal starting point for a reflection on the social uses of cultural heritage in Twentieth Century Argentina. The House reflects an aesthetic program that intended to reinvent the national tradition as a fusion between indigenous and Hispanic elements. Nonetheless, the national tradition codified by Rojas and Guido was the result of an arbitrary selection of heterogeneous elements that excluded immigrants and did not guarantee access to cultural heritage of all sectors of society. The project of the House was directly related to the emergence of cultural and politic nationalism that took place in Argentina from the Centenary of Independence in 1910 to the beginning of Second World War. This essay focuses on the contradictions of monumentalization of intangible cultural heritage in a multicultural society.

Interactions between sewage sludge-amended soil and earthworms--comparison between Eisenia fetida and Eisenia andrei composting species.

Science.gov (United States)

Rorat, Agnieszka; Suleiman, Hanine; Grobelak, Anna; Grosser, Anna; Kacprzak, Małgorzata; Płytycz, Barbara; Vandenbulcke, Franck

2016-02-01

Vermicomposting is an eco-friendly technology, where earthworms are introduced in the waste, inter alia sewage sludge, to cooperate with microorganisms and enhance decomposition of organic matter. The main aims of the present study was to determine the influence of two different earthworm species, Eisenia fetida and Eisenia andrei, on the changes of selected metallic trace elements content in substratum during vermicomposting process using three different sewage sludge mainly differentiated by their metal contents. Final vermicompost has shown a slight reduction in Cd, Cu, Ni, and Pb, while the Zn concentration tends to increase. Accumulation of particular heavy metals in earthworms' bodies was assessed. Both species revealed high tendency to accumulate Cd and Zn, but not Cu, Ni, and Pb, but E. andrei has higher capabilities to accumulate some metals. Riboflavin content, which content varies depending on metal pollution in several earthworms species, was measured supravitaly in extruded coelomocytes. Riboflavin content decreased slightly during the first 6 weeks of exposure and subsequently restored till the end of the 9-week experiment. Selected agronomic parameters have also been measured in the final product (vermicompost) to assess the influence of earthworms on substratum.

Cruz Roja Española: el trabajo con refugiados desde Cruz Roja Alicante (Spanish Red Cross: work with refugees from Alicante Red Cross

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Martínez Sánchez, Mar

2010-06-01

Full Text Available Resumen: Cruz Roja surge a raíz de un conflicto bélico con la finalidad de socorrer a las víctimas del mismo y localizar, mediante voluntarios, a los familiares de éstas. Con el paso de los años, la respuesta de Cruz Roja se institucionaliza y se amplia a las diferentes necesidades que emergen de la sociedad. Para uno de los colectivos más vulnerables, como son los refugiados, se crea un Programa específico para dar cobertura a sus necesidades más básicas durante el proceso de protección internacional, acompañándolos en las diferentes dificultades que se les plantea para alcanzar su integración en la ciudad.Abstract: Red Cross comes up with the aim to help victims from an armed conflict, and to locate their families trough volunteers. Trough the years, the Red Cross answer is widen to the different needs rising up in the society. From one of the most vulnerable group, refugees, a specific program has made to cover their basics needs during their international protection process, being with them in the different difficulties found to get integrated in the city.

LBP/BPI homologue in Eisenia andrei earthworms

Czech Academy of Sciences Publication Activity Database

Škanta, František; Procházková, Petra; Roubalová, Radka; Dvořák, Jiří; Bilej, Martin

2016-01-01

Roč. 54, č. 1 (2016), s. 1-6 ISSN 0145-305X R&D Projects: GA MŠk(CZ) ED1.1.00/02.0109; GA MŠk(CZ) EE2.3.30.0003 Institutional support: RVO:61388971 Keywords : Earthworm * Eisenia * LBP/BPI Subject RIV: EC - Immunology Impact factor: 3.218, year: 2016

Relationship between hemolytic molecules in Eisenia fetida earthworms

Czech Academy of Sciences Publication Activity Database

Kohlerová, Petra; Šilerová, Marcela; Felsberg, Jürgen; Josková, Radka; Beschin, A.; De Baetselier, P.; Bilej, Martin

2006-01-01

Roč. 30, - (2006), s. 381-392 ISSN 0145-305X R&D Projects: GA ČR GA310/04/0806 Institutional research plan: CEZ:AV0Z50200510 Keywords : fetidin * lysenin * eisenia Subject RIV: CD - Macromolecular Chemistry Impact factor: 3.399, year: 2006

Comunicación científica e innovación tecnológica en la primera Cruz Roja, 1863-1876

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Juan Carlos García-Reyes

Full Text Available Resumen Los primeros años del movimiento internacional de Cruz Roja coincidieron con grandes cambios tecnológicos en la medicina de guerra. Las peculiaridades organizativas de la Asociación Internacional de Socorro a los Soldados Heridos en Campaña, impulsada por el Comité de Ginebra y los comités nacionales de Cruz Roja; la confluencia en diversos congresos profesionales y publicaciones de médicos de las diferentes sociedades nacionales de esta asociación; y la construcción de un conocimiento práctico compartido, puesto a prueba en la Guerra Franco-Prusiana (1870-1871, proporcionan claves para comprender las innovaciones tecnológicas introducidas por la Cruz Roja Española durante la tercera y última Guerra Carlista (1872-1876.

El Área de Contabilidad de la UCLM y José Antonio Rojas

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Francisco Javier Quesada Sánchez

2009-06-01

Full Text Available Breve historia de los cinco primeros cursos del Área de Contabilidad de la Universidad de Castilla-La Mancha con especial referencia a la dedicación y protagonismo del Profesor José Antonio Rojas Tercero, a quien se rinde homenaje.

Ecotoxicidad de nanopartículas metálicas y superparamagnéticas de óxido de hierro en dos especies

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Yordanka Domínguez Linares

2017-10-01

Full Text Available Introducción: la nanotecnología y el empleo de materiales a nano escala son un área relativamente nueva de la ciencia y la tecnología con un gran crecimiento en el mercado global. Muchos de los productos no cuentan con estudios que garanticen su uso seguro, tanto para el hombre como para los ecosistemas. Los estudios ecotoxicológicos permiten evaluar los efectos de un determinado xenobiótico sobre especies representativas de los diferentes compartimentos ambientales. Objetivo: evaluar los efectos tóxicos de nanopartículas de Ag, Au, Ag/Ag y superparamagnéticas de óxido de hierro, en dos especies bioindicadoras de los ecosistemas terrestre y acuático. Métodos: como parte de los estudios de seguridad se realizaron ensayos de toxicidad aguda por contacto en lombriz de tierra de la especie Eisenia andrei, con una duración de 96 horas y estudios en anfibios de la especie Osteopillus septentrionales en diferentes etapas del desarrollo (embrionario y larval. Se evaluó la ocurrencia de mortalidad y de efectos tóxicos, en el caso del ensayo en lombriz de tierra; se determinó además la viabilidad celular. Resultados: los efectos tóxicos más significativos en el caso de la lombriz de tierra fueron, la ocurrencia de alteraciones fisiológicas y conductuales al ser expuesta a NPs de Ag de 3 nm y superparamagnéticas de óxido de hierro, estas últimas provocaron citotoxicidad a la concentración 1,38 mg/mL. En el caso de los anfibios se evidenció toxicidad en NPs de Ag 3 nm y superparamagnéticas de óxido de hierro. Conclusiones: todas las nanopartículas mostraron efectos tóxicos en las especies bioindicadoras evaluadas.

Uso de lombricompuestos en la producción comercial del Crisantemo Chrysanthemum morifolium Ramat

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Gómez Zambrano Jairo

1995-06-01

Full Text Available

Residues of crisantemos, clavel, statice and kikuyo grass alone or with cow dug and mixture of all they, were composted with aid of earthworm Eisenia fetida. These lombricomposts were tested versus soluble fertilization in the crisantemo crop. The best chemical and biological quality of the lombricomposts from flower residue were obtained with the admendment of cow dug, The bether yields and quality of crisantemo flower were obtained with lombricompost origined in flower residues with cow dug. The soluble fertilization was superior over the lombricomposts.

Estudio de los galactanos del alga roja pterocladiella Capillacea

OpenAIRE

Errea, María Inés

2001-01-01

Existen dos familias de polisacáridos sintetizados por algas rojas, los carragenanos y el ágar, que, debido a sus propiedades reológicas, que les permiten estabilizar soluciones o emulsiones acuosas, son utilizados en la industria farmacéutica y alimentaria de todo el mundo. Este Trabajo de Tesis comprende los tópicos que se indican a continuación: a) Estudio de la estructura de los galactanos que componen el sistema de polisacáridos de la agarofita Plerocladíella capillacea (Gelidiales, Geli...

Vermikompos Sampah Kebun dengan Menggunakan Cacing Tanah Eudrilus eugeneae dan Eisenia fetida

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Etik Rahmawati

2016-04-01

Full Text Available Durasi yang panjang diperlukan dalam pengomposan konvensional sampah organik yang memerlukan waktu selama 2-3 bulan. Pengurangan waktu pengomposan dapat dilakukan dengan digunakannya cacing sebagai dekomposer. Penelitian ini bertujuan untuk menentukan tingkat degradasi sampah kebun menggunakan proses vermikomposting dan menentukan pengaruh jenis cacing Eudrilus eugeneae dan Eisenia fetida. Empat reaktor berukuran 8 L digunakan dalam penelitian ini. Percobaan dilakukan secara duplo selama 60 hari. Parameter yang dianalisis pada penelitian ini adalah ammonia nitrogen (NH3-N, nitrat nitrogen (NO3-N, Total Kjeldahl Nitrogen (TKN, dan C/N. Hasil penelitian menunjukkan bahwa tingkat degradasi sampah kebun dengan pengolahan vermikomposting yang dapat dicapai adalah 64,94-72,52%. Produksi kompos yang lebih tinggi dengan penggunaan Eisenia fetida.

The Rise and Development of Physics in Cuba: An Interview with Hugo Pérez Rojas in May 2009

Science.gov (United States)

Baracca, Angelo

Hugo Celso Pérez Rojas was born in 1938, and works as a senior researcher at the Institute of Cybernetics, Mathematics and Physics, at the Ministry of Science and Technology, Cuba. Pérez Rojas is emeritus member of the Academy of Sciences of Cuba, member of the Latin American Academy of Sciences and Fellow TWAS since 1994. He was one of the founders of the School of Physics in the University of Havana in 1962, and moved in 1971 to the Cuban Academy of Sciences. His national awards include the Rafael Maria Mendive and Carlos J. Finlay Medals. He was awarded in 2011 the National Prize in Physics from the Cuban Physical Society. His interests include quantum field theory and its applications to finite temperature problems in high-energy physics and condensed matter. Among these, Pérez Rojas has devoted special attention to quantum electrodynamics in matter and in vacuum in the presence of external fields, phase transitions in electroweak theory, relativistic quantum Hall effect, Bose-Einstein condensation in magnetic fields, and applications of physics to social sciences. He is interviewed here by Angelo Baracca in May 2009.

Microbial environment affects innate immunity in two closely related earthworm species Eisenia andrei and Eisenia fetida.

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Jiří Dvořák

Full Text Available Survival of earthworms in the environment depends on their ability to recognize and eliminate potential pathogens. This work is aimed to compare the innate defense mechanisms of two closely related earthworm species, Eisenia andrei and Eisenia fetida, that inhabit substantially different ecological niches. While E. andrei lives in a compost and manure, E. fetida can be found in the litter layer in forests. Therefore, the influence of environment-specific microbiota on the immune response of both species was followed. Firstly, a reliable method to discern between E. andrei and E. fetida based on species-specific primers for cytochrome c oxidase I (COI and stringent PCR conditions was developed. Secondly, to analyze the immunological profile in both earthworm species, the activity and expression of lysozyme, pattern recognition protein CCF, and antimicrobial proteins with hemolytic function, fetidin and lysenins, have been assessed. Whereas, CCF and lysozyme showed only slight differences in the expression and activity, fetidin/lysenins expression as well as the hemolytic activity was considerably higher in E. andrei as compared to E. fetida. The expression of fetidin/lysenins in E. fetida was not affected upon the challenge with compost microbiota, suggesting more substantial changes in the regulation of the gene expression. Genomic DNA analyses revealed significantly higher level of fetidin/lysenins (determined using universal primer pairs in E. andrei compared to E. fetida. It can be hypothesized that E. andrei colonizing compost as a new habitat acquired an evolutionary selection advantage resulting in a higher expression of antimicrobial proteins.

Feeding activity of the earthworm Eisenia andrei in artificial soil.

NARCIS (Netherlands)

Jager, D.T.; Fleuren, R.H.L.J.; Roelofs, W.; de Groot, A.C.

2003-01-01

Quantitative information on the feeding activity of earthworms is scarce but this information is valuable in many eco(toxico)logical studies. In this study, the feeding activity of the compost worm Eisenia andrei is examined in artificial soil (OECD medium), with and without a high-quality food

Earthworm symbiont Verminephrobacter eiseniae mediates natural transformation within the host egg capsules using type IV pili

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SEANA Kelyn DAVIDSON

2014-10-01

Full Text Available The dense microbial communities commonly associated with plants and animals should offer many opportunities for horizontal gene transfer (HGT through described mechanisms of DNA exchange including natural transformation. However, studies of the significance of natural transformation have focused primarily on pathogens. The study presented here demonstrates highly efficient DNA exchange by natural transformation in a common symbiont of earthworms. The obligate bacterial symbiont Verminephrobacter eiseniae is a member of a microbial consortium of the earthworm Eisenia fetida that is transmitted into the egg capsules to colonize the embryonic worms. In the study presented here, by testing for transformants under different conditions in culture, we demonstrate that V. eiseniae can incorporate free DNA from the environment, that competency is regulated by environmental factors, and that it is sequence specific. Mutations in the type IV pili of V. eiseniae resulted in loss of DNA uptake, implicating the type IV pilus (TFP apparatus in DNA uptake. Furthermore, injection of DNA carrying antibiotic-resistance genes into egg capsules resulted in transformants within the capsule, demonstrating the relevance of DNA uptake within the earthworm system. The ability to take up species-specific DNA from the environment may explain the maintenance of the relatively large, intact genome of this long-associated obligate symbiont, and provides a mechanism for acquisition of foreign genes within the earthworm system.

COMPARACIÓN SENSORIAL DE TRES FORMULACIONES DE HAMBURGUESAS ELABORADAS A BASE DE TILAPIA ROJA (Oreochromis sp..

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JOSÉ IGOR HLEAP ZAPATA

2013-12-01

Full Text Available Se evaluaron sensorialmente hamburguesas elaboradas a base de tilapia roja y dos formulaciones adicionales, en las cuales se sustituyó un porcentaje de tilapia roja por carne de res, en una, y de cerdo, en la otra. Se plantearon tres formulaciones diferentes: solo de carne de tilapia roja codificada con la cifra aleatoria 8204 y carne de tilapia adicionadas con carne de res y carne de cerdo, identificadas con las cifras 6271 y 3522 respectivamente. Las hamburguesas se sometieron a degustaciones realizadas por un panel de 100 consumidores no entrenados, utilizando una escala hedónica de siete puntos. Se valoró estadísticamente la aprobación de los productos y se determinó que hubo buen agrado y buena aceptación. Los resultados indicaron una conformidad del 75% para la muestra 8204, mientras que para las muestras 6271 y 3522, ésta fue respectivamente de 78% y 82%. A pesar de que se presentaron algunas diferencias entre el porcentaje de aceptación de las tres formulaciones, el intervalo de varianza no es significativo, lo cual permitió concluir que los tres productos analizados fueron de completa satisfacción. Se concluyó que las diferencias entre las hamburguesas elaboradas solo a base de tilapia versus las elaboradas a base de tilapia con adición de otras carnes no influyeron en la aceptación por los consumidores.

Estrategia de comunicación de Creu Roja a Catalunya en situación de emergencia, crisis o desastre

OpenAIRE

Torralba Pouso, Margarita

2014-01-01

El propòsit d'aquest treball és el d'establir l'estratègia de comunicació de Creu Roja a Catalunya a l'hora de declarar-se una situació d'emergència, crisis o desastre a qualsevol dels nivells d'actuació de la institució humanitària. El propósito de este trabajo es el de establecer la estrategia de comunicación de Cruz Roja en Cataluña al declararse una situación de emergencia, crisis o desastre en cualquiera de los niveles de actuación de la institución humanitaria. The aim of this pro...

Evidence for proteins involved in prophenoloxidase cascade Eisenia fetida earthworms

Czech Academy of Sciences Publication Activity Database

Kohlerová, Petra; Šilerová, Marcela; Stijlemans, B.; Dieu, M.; Halada, Petr; Josková, Radka; Beschin, A.; De Baetselier, P.; Bilej, Martin

2006-01-01

Roč. 176, - (2006), s. 581-587 ISSN 0174-1578 R&D Projects: GA ČR GA310/04/0806; GA AV ČR KJB500200613 Institutional research plan: CEZ:AV0Z50200510 Keywords : protein * prophenoloxidase cascade * eisenia fetida Subject RIV: EE - Microbiology, Virology Impact factor: 1.740, year: 2006

Interactions between microfungi and Eisenia andrei (Oligochaeta) during cattle manure vermicomposting

Czech Academy of Sciences Publication Activity Database

Pižl, Václav; Nováková, Alena

2003-01-01

Roč. 47, - (2003), s. 895-899 ISSN 0031-4056 R&D Projects: GA AV ČR IAA6066001 Institutional research plan: CEZ:AV0Z6066911 Keywords : Eisenia andrei * earthworms * microscopic fungi Subject RIV: EH - Ecology, Behaviour Impact factor: 0.711, year: 2003

Efecto del proceso de secado de la lombriz de tierra (Eisenia andrei sobre el perfil aminoacídico de la harina determinado por cromatografía (CLAE | Effect of drying process of the earthworm (Eisenia andrei on amino acidic profile of the meal determined by chromatography (HPLC

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José Ovalles

2017-11-01

Full Text Available The possible effect of the drying temperature of the earthworm, Eisenia andrei, biomass on the amino acid profile of the resulting flour was evaluated. A tray dryer with air recirculation was used as a drying technique. The tested drying temperature were relatively low, T1 = 30, T2 = 40, T3 = 45 (°C, until obtaining a relative humidity below 10%. The differences in protein content of meal among treatments were not significant and resulted in average 63 ± 2% on a dry basis, and practically did not affect the proximate analysis in terms of total fat (7.2 ± 0.7% and ash content (5.3 ± 0.6%. Differences in the amino acid profile of the earthworm meal were not significant (p = 0.05 among treatments, except for asparagine (as Asp and glutamine (as Glu. Although, under the experimental conditions of the proposed approach, the results in terms of nutritional values were expected and satisfactory, they were not convenient in terms of microbiological quality. The results allow to infer that the use of low temperature drying is not a recommended approach to obtain earthworm meal, unless experimental conditions also ensure the microbiological quality without compromising the cost-benefit of the proposed drying process.

Eisenia fetida increased removal of polycyclic aromatic hydrocarbons from soil

Energy Technology Data Exchange (ETDEWEB)

Contreras-Ramos, Silvia M. [Laboratory of Soil Ecology, Department of Biotechnology and Bioengineering, Cinvestav, Av. Instituto Politecnico Nacional 2508, C.P. 07000 Mexico, D.F. (Mexico); Alvarez-Bernal, Dioselina [Laboratory of Soil Ecology, Department of Biotechnology and Bioengineering, Cinvestav, Av. Instituto Politecnico Nacional 2508, C.P. 07000 Mexico, D.F. (Mexico); Dendooven, Luc [Laboratory of Soil Ecology, Department of Biotechnology and Bioengineering, Cinvestav, Av. Instituto Politecnico Nacional 2508, C.P. 07000 Mexico, D.F. (Mexico)]. E-mail: dendoove@cinvestav.mx

2006-06-15

The removal of phenanthrene, anthracene and benzo(a)pyrene added at three different concentrations was investigated with or without earthworms (Eisenia fetida) within 11 weeks. Average anthracene removal by the autochthonous micro-organisms was 23%, 77% for phenanthrene and 13% for benzo(a)pyrene, while it was 51% for anthracene, 47% for benzo(a)pyrene and 100% for phenanthrene in soil with earthworms. At 50 and 100 mg phenanthrene kg{sup -1} E. fetida survival was 91% and 83%, but at 150 mg kg{sup -1} all died within 15 days. Survival of E. fetida in soil amended with anthracene {<=}1000 mg kg{sup -1} and benzo(a)pyrene {<=}150 mg kg{sup -1} was higher than 80% and without weight loss compared to the untreated soil. Only small amounts of PAHs were detected in the earthworms. It was concluded that E. fetida has the potential to remove large amounts of PAHs from soil, but more work is necessary to elucidate the mechanisms involved. - Addition of earthworms, Eisenia fetida, accelara removal of polycyclic aromatic hydrocarbons from soil.

Eisenia fetida increased removal of polycyclic aromatic hydrocarbons from soil

International Nuclear Information System (INIS)

Contreras-Ramos, Silvia M.; Alvarez-Bernal, Dioselina; Dendooven, Luc

2006-01-01

The removal of phenanthrene, anthracene and benzo(a)pyrene added at three different concentrations was investigated with or without earthworms (Eisenia fetida) within 11 weeks. Average anthracene removal by the autochthonous micro-organisms was 23%, 77% for phenanthrene and 13% for benzo(a)pyrene, while it was 51% for anthracene, 47% for benzo(a)pyrene and 100% for phenanthrene in soil with earthworms. At 50 and 100 mg phenanthrene kg -1 E. fetida survival was 91% and 83%, but at 150 mg kg -1 all died within 15 days. Survival of E. fetida in soil amended with anthracene ≤1000 mg kg -1 and benzo(a)pyrene ≤150 mg kg -1 was higher than 80% and without weight loss compared to the untreated soil. Only small amounts of PAHs were detected in the earthworms. It was concluded that E. fetida has the potential to remove large amounts of PAHs from soil, but more work is necessary to elucidate the mechanisms involved. - Addition of earthworms, Eisenia fetida, accelara removal of polycyclic aromatic hydrocarbons from soil

Evaluación de algunos parámetros fisicoquímicos y nutricionales en humus de lombriz y composts derivados de diferentes sustratos

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Myriam Rocío Melgarejo

2010-07-01

Full Text Available Se determinaron algunas propiedades fisicoquímicas y nutricionales en cuatro humus de lombriz obtenidos a partir de desechos de cocina y de huerta, pulpa de café, basuras biodegradables y residuos de rosas y en dos composts provenientes de residuos de rosas y de clavel. Las propiedades evaluadas fueron: humedad de campo, pH en agua (1:5, conductividad eléctrica (1:5, cenizas, materia orgánica, sílice (SiOj y capacidad de intercambio cationico por el método de extracción con acetato de amonio IN pH 7,0 y por el método de Harada. Así mismo, se determinó el contenido de hidrosolubles y su efecto sobre la germinación de semillas de Berro (Lepidium sativum; finalmente se realizó un análisis nutricional que comprendió la cuantificación de los elementos nutrientes en su forma total y disponible.

Gonzalo Rojas: la infancia del poeta en Lebu. Una unidad didáctica por tareas

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Puga Larraín, Juana

2011-06-01

Full Text Available Suplemento del número 12 de marcoELE (enero - junio de 2011 La unidad didáctica Gonzalo Rojas: la infancia del poeta en Lebu fue mi trabajo final del seminario “Enfoque por Tareas: programaciones y metodología”, dictado por Sheila Estaire, en el Programa de Máster en Enseñanza del Español como Lengua Extranjera (MEELE 2004-2005, de la Universidad Antonio de Nebrija, en Madrid. Posteriormente, a partir de ese trabajo, y bajo la dirección de Estaire, realicé mi Memoria de Máster: “Los principios del enfoque por tareas y su aplicación en la programación de la Unidad Didáctica Gonzalo Rojas: la infancia del poeta en Lebu “. Este libro corresponde a esa Memoria. En la primera parte, se revisan los aspectos teóricos; después de dar una mirada al Enfoque Comunicativo que da origen al Enfoque por Tareas, se presentan los fundamentos de éste último. En la segunda parte, se describen las Tareas que componen esta Unidad Didáctica. En la tercera parte, damos cuenta de la implementación de la Unidad Didáctica, el año 2005, en un curso del Programa de Lengua y Cultura de la Universidad de California en Concepción, al que asistieron veintidós estudiantes de diversas carreras de esa universidad. Finalmente, en los anexos, entregamos los resultados del trabajo de los estudiantes y el material necesario para implementar la unidad. La unidad didáctica Gonzalo Rojas: la infancia del poeta en Lebu, comienza con la presentación de mi documental Gonzalo Rojas: la casa, el fuego, el río; y con el análisis del poema Carbón, en el que el poeta habla de su infancia en Lebu y de su padre minero. Las primeras dieciocho tareas están destinadas a conocer al poeta, su pueblo natal, y a profundizar en el texto del poema. Posteriormente, hay tres tareas que buscan establecer una relación entre los poemas Carbón y El padre, de Pablo Neruda; y entre los primeros años de vida de ambos poetas. La tarea final de la unidad didáctica consiste en

María Rojas Tejada. La mujer moderna y la educación de la mujer en el siglo XX

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María Victoria Tipiani L

2015-08-01

Full Text Available María Rojas Tejada hizo parte importante de un proceso que fue fundamental y protagónico para Colombia al despertar del siglo XX como el de la educación, ayudando a la introducción de ideas extranjeras inscritas en la corriente llamada escuela nueva, que chocaban con las ideas tradicionales en el país. Este papel nunca estuvo desligado de su lucha por el derecho de las mujeres a educarse, teniendo en cuenta que la Modernidad y la introducción de la mujer al mundo laboral le planteaban nuevos retos y le abrían nuevas perspectivas educativas. Si bien el lugar de la mujer en la sociedad seguía basándose en una visión determinista, que permanece hoy en algunos sectores de la sociedad, María Rojas no solo tenía esa perspectiva de mujer moderna, sino que tomaba la palabra en representación de las mujeres, rompiendo paradigmas e impulsando nuevas ideas en torno a ellas. María Rojas Tejada was an important part of a fundamental and leading educational process for Colombia early in the 20th century, helping with the introduction of foreign ideas coming from a current called new school; these ideas were contrary to the traditional ones of the country. Her role was never apart from her fight for the woman’s right to be education, bearing in mind that Modernity and introduction of women in the labor world represented new challenges and opened new educational perspectives for them. Despite the woman’s place in society was based on a deterministic view that is still seen in our society, María Rojas not only had the idea of a modern woman but raised her voice on behalf of women, breaking paradigms and promoting new ideas around women

Effects of silver nanoparticles to soil invertebrates: Oxidative stress biomarkers in Eisenia fetida

International Nuclear Information System (INIS)

Gomes, Susana I.L.; Hansen, Ditte; Scott-Fordsmand, Janeck J.; Amorim, Mónica J.B.

2015-01-01

Silver nanoparticles (Ag-NPs) are among the most produced NPs worldwide having several applications in consumer products. Ag-NPs are known to cause oxidative stress in several organisms and cell lines, however comparatively less information is available regarding their effects on soil living invertebrates. The purpose of this study was to investigate if Ag-NPs cause oxidative stress on soil invertebrates. The model soil species Eisenia fetida was used. Our results showed that total glutathione (TG) is the first mechanism triggered by Ag-NPs, followed by glutathione peroxidase (GPx) and glutathione reductase (GR), however oxidative damage was observed for higher doses and exposure time (increased lipid peroxidation, LPO). AgNO 3 exposure caused impairment in GPx and glutathione-S-transferase (GST), probably as result of the higher bioavailability of Ag in the salt-form. The current results indicate that effects are partly caused by Ag ions released from Ag-NPs, but specific particle effects cannot be excluded. - Highlights: • Oxidative stress of Ag-NPs and AgNO 3 was assessed in Eisenia fetida. • Both Ag forms induced oxidative damage (LPO) via different mechanisms. • Ag-NPs activated total glutathione, followed by GPx and GR. • AgNO 3 impaired GPx and GST. • Overall results indicated effects from Ag ionization and NPs specific effects. - Oxidative stress to Ag in Eisenia fetida occurs via different mechanisms for Ag nanoparticles and AgNO 3

Tras ser desfigurado, Francisco de Rojas Zorrilla entra en el parnaso español. Los siglos XVIII y XIX

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Álvarez Barrientos, Joaquín

2007-06-01

Full Text Available The article studies the presence of the theater of Francisco de Rojas in the Spanish scenes of centuries XVIII and XIX, as well as the way in which the critical and historians of Literature valued that author. The refundiciones of their works, their relation with Fernandez de Moratín and their entrance in the national Parnaso are detailed.El artículo estudia la presencia del teatro de Francisco de Rojas en los escenarios españoles de los siglos XVIII y XIX, así como el modo en que los críticos e historiadores de la literatura valoraron a ese autor. Se detallan las refundiciones de sus obras, su relación con Fernández de Moratín y su entrada en el Parnaso nacional.

Molecular cloning and expression of TLR in the Eisenia andrei earthworm

Czech Academy of Sciences Publication Activity Database

Škanta, František; Roubalová, Radka; Dvořák, Jiří; Procházková, Petra; Bilej, Martin

2013-01-01

Roč. 41, č. 4 (2013), s. 694-702 ISSN 0145-305X R&D Projects: GA MŠk(CZ) EE2.3.20.0055; GA MŠk(CZ) EE2.3.30.0003 Institutional support: RVO:61388971 Keywords : Eisenia andrei * Innate immunity * Toll-like receptor Subject RIV: EC - Immunology Impact factor: 3.705, year: 2013

Efectos subletales de la lambda-cialotrina sobre Eisenia fetida (Annelida, Oligochaeta, Lumbricidae Sublethal effects of lambda-cyhalothrin on Eisenia fetida (Annelida, Oligochaeta, Lumbricidae

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Tamara Ricardo

2010-07-01

Full Text Available El uso intensivo de agroquímicos provoca efectos dañinos sobre la fauna no blanco. Dentro de la misma, los oligoquetos contribuyen a mantener la estructura y fertilidad del suelo. La lambda-cialotrina es uno de los insecticidas piretroides más utilizados en la Argentina, pero son escasos los datos existentes acerca de su toxicidad sobre oligoquetos. Los objetivos de este trabajo fueron evaluar mediante bioensayos de toxicidad crónica los efectos de lambda-cialotrina (producto comercial al 5% en Eisenia fetida sobre los parámetros de comportamiento, sobrevivencia, biomasa, reproducción y bioacumulación, así como la persistencia en suelo OECD de dicho tóxico. Los resultados muestran un comportamiento de huída intenso a partir de la concentración más baja, con un EC50 de 1,36 mg kg-1 (95% C.L. 0,24 - 2,80. No se observaron efectos en sobrevivencia y alimentación. La reproducción fue afectada significativamente (F= 11,94, PThe intensive use of agrochemicals has deleterious effects on non-target organisms. Among these organisms, earthworms are important because of their role in keeping the soil structure and fertility. Lambda-cyhalothrin is one of the most widely used pyrethroid insecticide in Argentina, but there are not enough studies of the effects of this pesticide on earthworms. The goals of this work were to perform chronic toxicity bioassays to determine the effects of commercial lambda-cyhalothrin (at 5% on Eisenia fetida. The parameters of behaviour, survival, biomass, reproduction, bioaccumulation and the degradation time of lambda-cyhalotrin in an OECD standard soil were also estimated. Results showed a hard avoidance behaviour in the lowest lambda-cyhalothrin concentration with an EC50 of 1.36 mg kg-1 (95% C.L 0.24-2.80. Eisenia fetida chronic test survival and feeding were not affected by lambda-cyhalothrin but negative effects on reproduction were significant (P<0.05. Cocoon production and fertility were reduced and

Evaluación de la toxicidad de cobre en suelos a través de biomarcadores de estrés oxidativo en eisenia foetida Assessment of copper toxicity in soils using biomarkers of oxidative stress in eisenia foetida

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Hernán Gaete

2010-01-01

Full Text Available Copper toxicity in soil was evaluated using biomarkers of oxidative stress (catalase enzyme activity, superoxide dismutase and lipid peroxidation in the earthworm Eisenia foetida. Agricultural topsoils from mining areas of the Aconcagua river basin were collected. Total copper concentrations were in the range of 94-959 mg kg-1, while the exchangeable copper concentrations were in the range of 46-2225 µg kg-1. Earthworms exposed to soil with exchangeable copper concentrations above 32 µg kg-1 showed an increase in catalase activity. Parameters of antioxidant activity were more sensitive than the weight change and thus can be used as appropriate biomarkers in Eisenia foetida.

Cristóbal de Rojas: new biographical information and first works

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Pedro Luengo

2018-05-01

Full Text Available The biography of the military engineer Cristóbal de Rojas was little known until now. This article aims to offer new data concerning his origins, possible formation and first appearance in Seville. Then his first documented work, the front doorway of the parish church of Cañete de las Torres (Cordoba, is analyzed. Thanks to this, it is possible to delimit his participation in the work of El Escorial to a contact with the mathematical circles of Juan de Herrera, as part of his training as an engineer with Tiburcio Spanocchi. Finally, this paper presents new information concerning his several marriages.

Toxische effecten van zink op de worm Eisenia andrei; resultaten proefveldexperimenten Amsterdam 1995 en 1996

NARCIS (Netherlands)

Bakker J; Posthuma L; Baerselman R; ECO/EEB

1997-01-01

De toxische effecten van zink voor de compostworm Eisenia andrei werden bestudeerd in twee proefveldexperimenten, om de veldrelevantie van laboratorium-toxiciteitsgegevens te onderzoeken indien blootstellingscondities op natuurlijke wijze fluctueren. Coconproductie werd hierbij gebruikt als

Rojas, Chaucer et Shakespeare au miroir de l'iconographie : les trois âges de la femme

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Sandra Gorgievski

2012-10-01

Full Text Available La mise en perspective de La Celestina de Fernando de Rojas, Troilus and Criseyde de Chaucer et Troilus and Cressida de Shakespeare met à jour des résonnances dans la parodie du triangle courtois (l’amoureux, la femme, l’entremetteur et la peinture du désenchantement du monde. Dans l’Europe médiévale et renaissante, l’image de la femme tentatrice peut aussi être mise en relation avec le thème iconographique des Trois âges de la femme et la Mort, mais aussi celui de la Tentation de saint Antoine (Hans Baldung, Jérôme Bosch, Pieter Huys, Joachim Patinir, mêlant sacré et profane, érotisme et morbidité. Au-delà des aspects tragiques, Chaucer célèbre l’amour terrestre et spirituel - dans sa célèbre rétractation, alors que la satire et le grotesque dominent chez Rojas et Shakespeare.Placing in parallel Fernando de Rojas’ La Celestina, Chaucer’s Troilus and Criseyde and Shakespeare’s Troilus and Cressida, one can find resonances in these works’ parody of the courtly triangle (the lovers, the go-between and their representation of a disenchanted world. In Medieval and Renaissance Europe, the image of the seductive woman can also be related to the iconographic themes of the Three Ages of Woman and Death and the Temptation of Saint Antonio (Hans Baldung, Hieronymus Bosch, Pieter Huys, Joachim Patinir, which mingle the sacred and the profane, the erotic and the morbid. Beyond tragic overtones, Chaucer celebrates both earthly and spiritual love in his famous retraction, while Rojas and Shakespeare highlight the grotesque and the satirical.

Analysis of Waste in the Production of Flour California Red Worm (eisenia foet in Manabí Technical Universitypilot Plant

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Ulbio Alcívar-Cedeño

2016-07-01

Full Text Available The necessity for have efficient tools in the environmental assessment of production processesis treated in this paper. This work is related to a previous work made in the Technical University of Manabi, forprotein supplements production from unconventional raw materials, specifically Earthworm (Eisenia foetidaflour, using various ecotoxicological methods to evaluategeneratedwaste in pilot production, in order to contribute to compliance the environmental regulations.Liquid wastes generated in the production of earthworm flour were determined and evaluated under the following biological indicators: inhibition of seed germination, root growth in Lactuca sativa, acute toxicity in Eisenia foetida to determine the environmental toxicity of the production process. As result, the major environmental contamination that occurs during the process of obtaining meal worm (Eisenia foetida is given in the cleaning process, sacrifice, washing and milling. The waste characterization allowed determining that they are domestic water and process water. The use of ecotoxicological bioassays described, that the sacrificialwater inhibit germination and root elongation, and they are classified as slightly toxic thewash waters cause sublethal effects.

"El lobo y los siete cabritillos" y "Caperucita roja", historia de una relación

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Susana Gonzàlez Marìn

2006-01-01

Full Text Available Desde hace tiempo se ha considerado que existe una relación entre "El lobo y los siete cabritillos¿ y "Caperucita Roja", especialmente si tenemos en cuenta las versiones de los hermanos Grimm. El germen de "El lobo y los siete cabritillos" se encuentra en una fábula incluida en colecciones de la Antigüedad Tardía y de la Edad Media. En cambio, "Caperucita Roja" posee una historia completamente distinta, ajena al género de la fábula, ligada ya en la antigüedad clásica y en época medieval a ritos de iniciación femenina. A pesar de que la comparación de ambas tradiciones arroja una luz nueva sobre la cuestión y demuestra que el origen de los dos cuentos es diferente, sin embargo la utilización de la fábula del lobo y el cabritillo en sermones medievales cristianos con una moraleja destinada a las muchachas demuestra que probablemente ya en esa época se produjo un acercamiento debido a la utilización pedagógica y moral que el cristianismo hizo de todo tipo de relatos.

Effects of a natural toxin on life history and gene expression of Eisenia andrei

NARCIS (Netherlands)

van Ommen Kloeke, A.E.E.; Gong, Ping; Ellers, J.; Roelofs, D.

2014-01-01

Earthworms perform key functions for a healthy soil ecosystem, such as bioturbation. The soil ecosystem can be challenged by natural toxins such as isothiocyanates (ITCs), produced by many commercial crops. Therefore, the effects of 2-phenylethyl ITC were investigated on the earthworm Eisenia andrei

Uptake pathways and toxicity of Cd and Zn in the earthworm Eisenia fetida.

NARCIS (Netherlands)

Li, L.-Z.; Zhou, D.-M.; Peijnenburg, W.J.G.M.; Wang, P.; van Gestel, C.A.M.; Jin, S.-Y.; Wang, Q.-Y.

2010-01-01

The uptake of Cd and Zn by the earthworm Eisenia fetida was determined at varying Ca concentrations and with pre-exposure to different metabolic inhibitors in simulated soil solutions over a 48-h period. The presence of Ca in the solution had complex actions on Cd uptake. At a low Cd concentration

Micromamíferos vallesienses del yacimiento La Salle en las Arcillas Rojas de Teruel

OpenAIRE

ADROVER, Rafael; ALCALÁ, Luís; MEIN, Pierre; MOISSENET, Etienne; PARICIO, Juan

1982-01-01

El hallazgo de unos restos de micromamiferos en las proximidades del Colegio La Salle de Teruel permite datar la formación de las arcillas rojas conocida con 10s nombres de "Los Monotos", "Formación de Los Tejares" y "Pera1 Formation". La ausencia casi total de fósiles ha hecho que la edad de esa formación fuera largamente discutida. Los fósiles recientemente encontrados permiten atribuirle una edad vallesiense (MN 10 basal). La fauna recogida comprende: Galerix (Parasorex) socialis, Crusafon...

Correlations of Eisenia fetida metabolic responses to extractable phenanthrene concentrations through time

Energy Technology Data Exchange (ETDEWEB)

McKelvie, Jennifer R.; Wolfe, David M.; Celejewski, Magda; Simpson, Andre J. [Department of Physical and Environmental Sciences, University of Toronto, 1265 Military Trail, Toronto, Ontario, M1C 1A4 (Canada); Simpson, Myrna J., E-mail: myrna.simpson@utoronto.c [Department of Physical and Environmental Sciences, University of Toronto, 1265 Military Trail, Toronto, Ontario, M1C 1A4 (Canada)

2010-06-15

Eisenia fetida earthworms were exposed to phenanthrene for thirty days to compare hydroxypropyl-beta-cyclodextrin (HPCD) extraction of soil and {sup 1}H NMR earthworm metabolomics as indicators of bioavailability. The phenanthrene 28-d LC{sub 50} value was 750 mg/kg (632-891, 95% confidence intervals) for the peat soil tested. The initial phenanthrene concentration was 319 mg/kg, which biodegraded to 16 mg/kg within 15 days, at which time HPCD extraction suggested that phenanthrene was no longer bioavailable. Multivariate statistical analysis of {sup 1}H NMR spectra for E. fetida tissue extracts indicated that phenanthrene exposed and control earthworms differed throughout the 30 day experiment despite the low phenanthrene concentrations present after 15 days. This metabolic response was better correlated to total phenanthrene concentrations (Q{sup 2} = 0.59) than HPCD-extractable phenanthrene concentrations (Q{sup 2} = 0.46) suggesting that {sup 1}H NMR metabolomics offers considerable promise as a novel, molecular-level method to directly monitor the bioavailability of contaminants to earthworms in the environment. - Metabolic responses of Eisenia fetida earthworms to phenanthrene exposure are better correlated to total phenanthrene concentrations than to cyclodextrin-extractable concentrations through time.

Correlations of Eisenia fetida metabolic responses to extractable phenanthrene concentrations through time

International Nuclear Information System (INIS)

McKelvie, Jennifer R.; Wolfe, David M.; Celejewski, Magda; Simpson, Andre J.; Simpson, Myrna J.

2010-01-01

Eisenia fetida earthworms were exposed to phenanthrene for thirty days to compare hydroxypropyl-β-cyclodextrin (HPCD) extraction of soil and 1 H NMR earthworm metabolomics as indicators of bioavailability. The phenanthrene 28-d LC 50 value was 750 mg/kg (632-891, 95% confidence intervals) for the peat soil tested. The initial phenanthrene concentration was 319 mg/kg, which biodegraded to 16 mg/kg within 15 days, at which time HPCD extraction suggested that phenanthrene was no longer bioavailable. Multivariate statistical analysis of 1 H NMR spectra for E. fetida tissue extracts indicated that phenanthrene exposed and control earthworms differed throughout the 30 day experiment despite the low phenanthrene concentrations present after 15 days. This metabolic response was better correlated to total phenanthrene concentrations (Q 2 = 0.59) than HPCD-extractable phenanthrene concentrations (Q 2 = 0.46) suggesting that 1 H NMR metabolomics offers considerable promise as a novel, molecular-level method to directly monitor the bioavailability of contaminants to earthworms in the environment. - Metabolic responses of Eisenia fetida earthworms to phenanthrene exposure are better correlated to total phenanthrene concentrations than to cyclodextrin-extractable concentrations through time.

Mujeres delincuentes e imaginarios. Criminología, cine y nota roja en México, 1940-1950

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MARTHA SANTILLÁN ESQUEDA

Full Text Available RESUMEN La modernización política y económica impulsada por los gobiernos tras la Revolución Mexicana, repercutió en la apertura de espacios para el desenvolvimiento de mujeres fuera del ámbito doméstico. La especialización de la criminología y la profesionalización de medios de comunicación, ligados a un fuerte conservadurismo, coadyuvaron en la conformación de un notorio temor ante tal fenómeno, asumiendo que ello pudiera provocar en las mujeres el relajamiento de la moral y, en consecuencia, conductas delictivas. El artículo analiza la conformación de imaginarios en torno a la mujer delincuente en un periodo de notorio auge tanto del cine como de la nota roja. Al presentar a las criminales como un ser monstruoso y perverso, imagen que no distaba mucho de las ideas criminológicas, se prolongaban temores existentes en torno a la delincuencia femenina pero también se fortalecía la concepción de una feminidad atada al espacio doméstico. Se estudian filmes, prensa nota roja, diarios de circulación nacional y artículos criminológicos.

Avoidance behaviour testing of Eisenia andrei in biodegradable plastic environment

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Zuzana Tichá

2013-01-01

Full Text Available Avoidance behaviour test with the earthworms (ISO 17512-1:2008 is a rapid screening test for the evaluation of soil and the influence of pollutants and chemicals on the behaviour of earthworms. The purpose of the testing is to determine the avoidance behaviour of earthworm (in this case Eisenia andrei was used which can be used as an organism for the composting and occur naturally in soil environment. The methodology was modified according to the needs of the avoidance behaviour testing of earthworms in biodegradable plastic environment. It is a biodegradable thermoplastic material Mater-Bi, which is produced from corn starch. Californian earthworm (Eisenia andrei was chosen as a test organism. The two-chamber test was used in testing. 10 earthworms were used, which were exposed to a number of concentrations of the test substance, which was mixed into the compost environment. It was recorded both a positive result, avoidance reaction, as well as a negative result, non-avoidance reaction and also there was a case, where individuals prefer both substrates equally. Organisms showed no escape reaction and were fairly evenly distributed in both halves of the test vessel, it can be assessed that organisms prefer both substrates equally. In testing, the mortality was zero, none of the individuals died, at the conclusion of the test there were not found any dead individuals. Avoidance higher than 80 % didn’t occur; it cannot be said that the substrate is toxic or degraded.

Toxicity of zinc oxide nanoparticles in the earthworm, Eisenia fetida and subcellular fractionation of Zn.

NARCIS (Netherlands)

Li, L.-Z.; Zhou, D.-M.; Peijnenburg, W.J.G.M.; van Gestel, C.A.M.; Jin, S.-Y.; Wang, Y.-J.; Wang, P.

2011-01-01

The extensive use of nanoparticles (NPs) in a variety of applications has raised great concerns about their environmental fate and biological effects. This study examined the impact of dissolved organic matter (DOM) and salts on ZnO NP dispersion/solubility and toxicity to the earthworm Eisenia

The material record of the Iberian site in Fonteta Ràquia (Riba-Roja, València | El registro material del colmenar ibérico de la Fonteta Ràquia (Riba-Roja, València

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David Quixal Santos

2016-12-01

Full Text Available The archaeological excavation of the site in Fonteta Ràquia (Riba-Roja del Túria, Valencia documented a small rural settlement from the Edetania, the territory where the ancient Iberian city of Edeta / Tossal de Sant Miquel (Llíria, Valencia was located. The site was occupied between the end of the 5th century BC and the end of the 3rd / beginning of the 2nd century B. It stands out, especially, for a high specialization in beekeeping, as evidenced by the discovery of thousands of fragments from about 200 pottery beehives, exceeding by far the previous records of this type of activity in Iberia and the Mediterranean Sea in general. | La excavación arqueológica en 2008 del yacimiento de la Fonteta Ràquia (Riba-Roja del Túria, València permitió documentar un pequeño asentamiento rural de la Edetania, territorio de la antigua ciudad ibérica de Edeta / Tossal de Sant Miquel (Llíria, València. El núcleo estuvo ocupado entre finales del s. V a.C. y finales del III / comienzos del II a.C. Destaca, por encima de todo, por una gran especialización en apicultura, tal y como demuestra el hallazgo de miles de fragmentos de aproximadamente 200 colmenas cerámicas, superando con creces los registros precedentes de este tipo de actividad en Iberia y el Mediterráneo en general.

Red worm behavior (Eisenia spp.) in vermicomposting systems of organic residues

OpenAIRE

Mamani-Mamani Gladys; Mamani-Pati Francisco; Sainz-Mendoza Humberto; Villca-Huanaco René

2012-01-01

This work evaluates the behavior of the vermicomposting red worm (Eisenia spp.) in two environments (greenhouse and unsheltered) and with two solid organic substrates (cow manure=CM and kitchen waste=KW) in the zone of Carmen Pampa, Nor Yungas Province, Department of La Paz – Bolivia, using a factorial design with two factors and three repetitions (ANOVA analysis). The largest number of cocoons was found in the greenhouse treatment, with 64 in CM and 41 in KW. Cocoon viability was also greate...

Earthworm (Eisenia andrei Avoidance of Soils Treated with Cypermethrin

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Mara M. de Andréa

2011-11-01

Full Text Available The pyrethroid insecticide cypermethrin is used for agricultural and public health campaigns. Its residues may contaminate soils and the beneficial soil organisms, like the earthworms, that may ingest the contaminated soil particles. Due to its ecological relevance, earthworms Eisenia andrei/fetida have been used in different ecotoxicological tests. The avoidance of soils treated with cypermethrin by compost worms Eisenia andrei was studied here as a bioindicator of the influence of treatment dosage and the pesticide formulation in three different agricultural soils indicated by the Brazilian environmental authorities for ecotoxicological tests. This earthworms’ behavior was studied here as a first attempt to propose the test for regulation purposes. The two-compartment test systems, where the earthworms were placed for a two-day exposure period, contained samples of untreated soil alone or together with soil treated with technical grade or wettable powder formulation of cypermethrin. After 48 h, there was no mortality, but the avoidance was clear because all earthworms were found in the untreated section of each type of soil (p < 0.05. No differences were found by the Fisher’s exact test (p ≤ 1.000 for each soil and treatment, demonstrating that the different soil characteristics, the cypermethrin concentrations and formulation, as well as the smaller amounts of soil and earthworms did not influence the avoidance behavior of the earthworms to cypermethrin. The number and range of treatments used in this study do not allow a detailed recommendation of the conditions applied here, but to the best of our knowledge, this is the first reported attempt to identify the avoidance of pesticide treated tropical soils by earthworms.

Diagnóstico clínico patológico de brotes de enfermedades en tilapia roja (oreochromis spp.)

OpenAIRE

Rey, A. L.; Iregui Castro, C. A.; Verján, N.

2012-01-01

Se diagnosticaron las distintas patologías en tilapia roja que fueron consultadas al servicio de diagnóstico del Laboratorio de Patología de la Facultad de medicina Veterinaria de la Universidad Nacional, describiendo y clasificando los tipos de alteraciones macro y microscópicas e intentando formular los primeros mapas epidemiológicos por región geográfica y para esta especie. (...)

Multiplicación de Selenicereus megalanthus (pitahaya amarilla e Hylocereus polyrhizus (pitahaya roja vía organogénesis somática

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Rocío Stella Suárez Román

2014-09-01

Full Text Available Pitahaya amarilla (Selenicereus megalanthus y roja (Hylocereus polyrhizus se multiplicaron vía organogénesis somática, a partir de hojas cotiledonares y fragmentos de cladodio provenientes de plántulas con 20 días de desarrollo, en medio Murashige & Skoog (MS suplementado con auxinas (ANA y citocininas (Kinetina y BAP. En el medio de cultivo M3 (BAP + Kinetina 2 mg/l se obtuvieron entre cuatro y nueve brotes enraizados de 4 cm de longitud, en un periodo de 30 días. En pitahaya roja se desarrolló mayor número de brotes. En todos los medios estudiados se observó formación de callo, siendo más alto el número en el medio con altas concentraciones de ANA. Las vitroplantas en fase de climatización alcanzaron 100% de sobrevivencia y presentaron respuestas diferenciales al sustrato empleado.

Vermistabilization of Municipal Wastewater Sludge with Eisenia fetida

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A Parvaresh, H Movahedian, L Hamidian

2004-10-01

Full Text Available Sludges are stabilized to reduce pathogens, eliminate offensive odors and inhibit, reduce or eliminate the potential for putrification. In this study, stabilization of municipal wastewater sludge with and without earthworms (Eisenia fetida was tested in a pilot study. The earthworms were fed at the optimum level of 0.75 kg-feed/kg-worm/day. Decomposition and stabilization of wastewater sludge occurred both in the presence and in the absence of earthworms during 9 weeks but the process was accelerated in their presence. Phosphorus content increased in the sludge with earthworms but decreased in it without them. Nitrogen content in the resulting vermicompost showed no difference with its quantity in the original substrate while it increased in the control treatment.

Lethal and sub-lethal effects of five pesticides used in rice farming on the earthworm Eisenia fetida

NARCIS (Netherlands)

Rico, Andreu; Sabater, Consuelo; Castillo, María Ángeles

2016-01-01

The toxicity of five pesticides typically used in rice farming (trichlorfon, dimethoate, carbendazim, tebuconazole and prochloraz) was evaluated on different lethal and sub-lethal endpoints of the earthworm Eisenia fetida. The evaluated endpoints included: avoidance behaviour after an exposure

Influence of soil properties on the bioaccumulation and effects of arsenic in the earthworm Eisenia andrei

NARCIS (Netherlands)

Romero Freire, A.; Martin Peinado, F.J.; Diez Ortiz, M.; van Gestel, C.A.M.

2015-01-01

This study aimed at assessing the influence of soil properties on the uptake and toxicity effects of arsenic in the earthworm Eisenia andrei exposed for 4 weeks to seven natural soils spiked with different arsenic concentrations. Water-soluble soil concentrations (AsW) and internal As concentrations

Variation in blade morphology of the kelp Eisenia arborea : incipient speciation due to local water motion?

NARCIS (Netherlands)

Roberson, L.M.; Coyer, J.A.

2004-01-01

The southern sea palm kelp Eisenia arborea produces wide, bullate (bumpy) blades in low-flow areas, whereas in adjacent high-flow areas blades are flat and narrow. Here we determine if morphological differences in these 2 closely associated populations are correlated with physical factors in the

Glycosaminoglycans from earthworms (Eisenia andrei).

Science.gov (United States)

Im, A-Rang; Park, Youmie; Sim, Joon-Soo; Zhang, Zhenqing; Liu, Zhenling; Linhardt, Robert J; Kim, Yeong Shik

2010-02-01

The whole tissue of the earthworm (Eisenia andrei) was lyophilized and extracted to purify glycosaminoglycans. Fractions, eluting from an anion-exchange column at 1.0 M and 2.0 M NaCl, showed the presence of acidic polysaccharides on agarose gel electrophoresis. Monosaccharide compositional analysis showed that galactose and glucose were most abundant monosaccharides in both fractions. Depolymerization of the polysaccharide mixture with glycosaminoglycan-degrading enzymes confirmed the presence of chondroitin sulfate/dermatan sulfate and heparan sulfate in the 2.0 M NaCl fraction. The content of GAGs (uronic acid containing polysaccharide) in the 2.0 M NaCl fraction determined by carbazole assay was 2%. Disaccharide compositional analysis using liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) analysis after chondroitinase digestion (ABC and ACII), showed that the chondroitin sulfate/dermatan sulfate contained a 4-O-sulfo (76%), 2,4-di-O-sulfo (15%), 6-O-sulfo (6%), and unsulfated (4%) uronic acid linked N-acetylgalactosamine residues. LC-ESI-MS analysis of heparin lyase I/II/III digests demonstrated the presence of N-sulfo (69%), N-sulfo-6-O-sulfo (25%) and 2-O-sulfo-N-sulfo-6-O-sulfo (5%) uronic acid linked N-acetylglucosamine residues.

EFECTO DEL VERMICOMPOSTAJE SOBRE LAS POBLACIONES DE Colletotrichum acutatum y Pectobacterium carotovorum PRESENTES EN RESIDUOS DE PLANTAS

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Lidieth Uribe

2009-01-01

Full Text Available Se evaluó el efecto del vermicompostaje en la eliminación del hongo Colletotrichum acutatum presente en residuos del helecho hoja de cuero (Rumohra adiantiformis y de la bacteria Pectobacterium carotovorum inoculada en residuos de papa (Solanum tuberosum. En ambos ensayos se utilizaron cajas de madera divididas en 3 compartimentos, en la sección central se colocó sustrato con lombrices (Eisenia foetida y en las secciones laterales el material infectado respectivo. Los compartimentos se dividieron con mallas que permitieron el paso de las lombrices al tratamiento de vermicompostaje pero no al testigo. Se determinó la presencia de C. acutatum en los residuos de helecho mediante recuentos semanales de conidias durante 70 días. La población de P. carotovorum en los residuos de papa se evaluó utilizando la técnica de recuento en plato en medios específicos a los 26 y a los 41 días, y por la técnica de número más probable (NMP al día 54. Si bien el número de esporas de C. acutatum disminuyó en ambos tratamientos, a partir del día 35, se observó una mayor reducción en el tratamiento con lombrices que en el tratamiento testigo. Hacia el final del experimento, el número de conidias se redujo 100 veces con respecto a la población original en el tratamiento vermicompostaje y 10 veces en el tratamiento testigo. En el caso de los desechos de papa, al día 41 la disminución en la población de P. carotovorum fue 100 veces mayor en el tratamiento de vermicompostaje que en el control, no detectándose la bacteria al día 54 en el tratamiento con lombrices. La reducción de las poblaciones de patógenos en el tratamiento de vermicompostaje se atribuye al efecto directo (degradación, e indirecto (cambios en pH, efecto cualitativo sobre poblaciones de microorganismos que la lombriz roja ejerce sobre los residuos infectados.

Bioavailability and chronic toxicity of bismuth citrate to earthworm Eisenia andrei exposed to natural sandy soil.

Science.gov (United States)

Omouri, Zohra; Hawari, Jalal; Fournier, Michel; Robidoux, Pierre Yves

2018-01-01

The present study describes bioavailability and chronic effects of bismuth to earthworms Eisenia andrei using OECD reproduction test. Adult earthworms were exposed to natural sandy soil contaminated artificially by bismuth citrate. Average total concentrations of bismuth in soil recovered by HNO 3 digestion ranged from 75 to 289mg/kg. Results indicate that bismuth decreased significantly all reproduction parameters of Eisenia andrei at concentrations ≥ 116mg/kg. However, number of hatched cocoons and number of juveniles seem to be more sensitive than total number of cocoons, as determined by IC 50 ; i.e., 182, 123 and > 289mg/kg, respectively. Bismuth did not affect Eisenia andrei growth and survival, and had little effect on phagocytic efficiency of coelomocytes. The low immunotoxicity effect might be explained by the involvement of other mechanisms i.e. bismuth sequestered by metal-binding compounds. After 28 days of exposure bismuth concentrations in earthworms tissue increased with increasing bismuth concentrations in soil reaching a stationary state of 21.37mg/kg dry tissue for 243mg Bi/kg dry soil total content. Data indicate also that after 56 days of incubation the average fractions of bismuth available extracted by KNO 3 aqueous solution in soil without earthworms varied from 0.0051 to 0.0229mg/kg, while in soil with earthworms bismuth concentration ranged between 0.310-1.347mg/kg dry soil. We presume that mucus and chelating agents produced by earthworms and by soil or/and earthworm gut microorganisms could explain this enhancement, as well as the role of dermal and ingestion routes of earthworms uptake to soil contaminant. Copyright © 2017 Elsevier Inc. All rights reserved.

Involvement of the iron regulatory protein from Eisenia andrei earthworms in the regulation of cellular iron homeostasis.

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Petra Procházková

Full Text Available Iron homeostasis in cells is regulated by iron regulatory proteins (IRPs that exist in different organisms. IRPs are cytosolic proteins that bind to iron-responsive elements (IREs of the 5'- or 3'-untranslated regions (UTR of mRNAs that encode many proteins involved in iron metabolism. In this study, we have cloned and described a new regulatory protein belonging to the family of IRPs from the earthworm Eisenia andrei (EaIRP. The earthworm IRE site in 5'-UTR of ferritin mRNA most likely folds into a secondary structure that differs from the conventional IRE structures of ferritin due to the absence of a typically unpaired cytosine that participates in protein binding. Prepared recombinant EaIRP and proteins from mammalian liver extracts are able to bind both mammalian and Eisenia IRE structures of ferritin mRNA, although the affinity of the rEaIRP/Eisenia IRE structure is rather low. This result suggests the possible contribution of a conventional IRE structure. When IRP is supplemented with a Fe-S cluster, it can function as a cytosolic aconitase. Cellular cytosolic and mitochondrial fractions, as well as recombinant EaIRP, exhibit aconitase activity that can be abolished by the action of oxygen radicals. The highest expression of EaIRP was detected in parts of the digestive tract. We can assume that earthworms may possess an IRE/IRP regulatory network as a potential mechanism for maintaining cellular iron homeostasis, although the aconitase function of EaIRP is most likely more relevant.

Involvement of the Iron Regulatory Protein from Eisenia andrei Earthworms in the Regulation of Cellular Iron Homeostasis

Science.gov (United States)

Procházková, Petra; Škanta, František; Roubalová, Radka; Šilerová, Marcela; Dvořák, Jiří; Bilej, Martin

2014-01-01

Iron homeostasis in cells is regulated by iron regulatory proteins (IRPs) that exist in different organisms. IRPs are cytosolic proteins that bind to iron-responsive elements (IREs) of the 5′- or 3′-untranslated regions (UTR) of mRNAs that encode many proteins involved in iron metabolism. In this study, we have cloned and described a new regulatory protein belonging to the family of IRPs from the earthworm Eisenia andrei (EaIRP). The earthworm IRE site in 5′-UTR of ferritin mRNA most likely folds into a secondary structure that differs from the conventional IRE structures of ferritin due to the absence of a typically unpaired cytosine that participates in protein binding. Prepared recombinant EaIRP and proteins from mammalian liver extracts are able to bind both mammalian and Eisenia IRE structures of ferritin mRNA, although the affinity of the rEaIRP/Eisenia IRE structure is rather low. This result suggests the possible contribution of a conventional IRE structure. When IRP is supplemented with a Fe-S cluster, it can function as a cytosolic aconitase. Cellular cytosolic and mitochondrial fractions, as well as recombinant EaIRP, exhibit aconitase activity that can be abolished by the action of oxygen radicals. The highest expression of EaIRP was detected in parts of the digestive tract. We can assume that earthworms may possess an IRE/IRP regulatory network as a potential mechanism for maintaining cellular iron homeostasis, although the aconitase function of EaIRP is most likely more relevant. PMID:25279857

Influence of temperature on the toxicity of zinc to the earthworm Eisenia fetida

Energy Technology Data Exchange (ETDEWEB)

Spurgeon, D.J.; Tomlin, M.A.; Hopkin, S.P. [Univ. of Reading (United Kingdom)

1997-02-01

A range of toxicity tests have been proposed to assess the potential hazards of pollutants to earthworms. Of these, the two acute toxicity tests using Eisenia fetida recommended by the OECD and EEC have become routinely used in the risk assessment and regulation of new and existing chemicals. In addition to the acute tests, procedures have also been proposed for measuring the sub-lethal effects of chemicals on parameter such as reproduction and weight change. In both the lethal and sub-lethal toxicity tests developed with worms, attempts have been made to standardise test conditions to allow results from different laboratories to be directly compared. However, variability in exposure conditions and responses are fundamental to determine the effects of pollutants under natural conditions. In the field, conditions such as light, moisture availability, pH, temperature and humidity all fluctuate over time. Such variations affect both the sensitivity and exposure of individuals to toxic chemicals. Hence when evaluating the potential effects of pollutants, it may be important to known how changes in test conditions influence toxicity. This study assessed the effects of different temperatures on the lethal and sub-lethal toxicity of zinc for the earthworm Eisenia fetida. 23 refs., 1 fig., 1 tab.

Physiological and molecular responses of the earthworm (Eisenia fetida) to soil chlortetracycline contamination

International Nuclear Information System (INIS)

Lin Dasong; Zhou Qixing; Xu Yingming; Chen Chun; Li Ye

2012-01-01

This study aims to evaluate toxic effects of exposure to chlortetracycline (CTC) in soil on reproductive endpoints (juvenile counts and cocoon counts), biochemical responses, and genotoxic potentials of the earthworm Eisenia fetida. Results showed that juvenile counts and cocoon counts of the tested earthworms were reduced after exposure to CTC. The effective concentrations (EC 50 values) for juvenile and cocoon counts were 96.1 and 120.3 mg/kg, respectively. Treatment of earthworms with CTC significantly changed the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST). An increase in malondialdehyde (MDA) indicated that CTC could cause cellular lipid peroxidation in the tested earthworms. The percentage of DNA in the tail of single-cell gel electrophoresis of coelomocytes as an indication of DNA damage increased after treatment with different doses of CTC, and a dose-dependent DNA damage of coelomocytes was found. In conclusion, CTC induces physiological responses and genotoxicity on earthworms. - Highlights: ► Reproductive endpoints were assessed for Eisenia fetida exposed to chlortectracyline (CTC). ► CTC may induce physiological and molecular responses in E. fetida. ► A clear relationship was observed between CTC doses and DNA damage of coelomocytes. - Chlortetracycline in soil could induce physiological responses and genotoxicity on earthworms at realistic environmental concentrations.

La formación Capas Rojas: caracterización y génesis

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Molina, J. M.

1999-04-01

Full Text Available The characteristics (lithofacies, biofacies, age, thickness, vertical and lateral relations, etc., and significance of the Capas Rojas Formation (Upper Cretaceous-Eocene are analysed. This formation crops out widely in the Subbetic (External Zones of the Betic Cordillera. The Capas Rojas Formation presents a great uniformity in the wide sectors of this cordillera and has its equivalent in facies and age, in the Scaglia Rossa Formation of the Apennines and southern Alps and also in equally analogous formations in other Alpine Mediterranean domains. From the available sedimentological and paleoecological data, mainly from the macrofossils (isolated rudists and echinoids content and from the ichnofacies analysis, it is concluded that its deposition took place in a hemipelagic marine environment with a moderate depth (a few hundreds of metres during a long interval of time (40-50 Ma with a very low sedimentation rate (a few millimetres per thousand years. From the analysis of the evolution of the continental margin in which was deposited, and of their equivalents in other Alpine domains, is deduced that the beginning of its deposition recorded the end of the differentiation in troughs and swells of these passive margins in the advanced stages of the rifting, so that this unit, in wide sector of the basin covered areas with an irregular topography in the bottom, mainly controlled by faults and slowly leveled them.Se analizan las características (litofacies, biofacies, edad, potencia, etc. de la Formación Capas Rojas (Cretácico superior-Eoceno que aflora extensamente en el Subbético, dentro de las Zonas Externas de la Cordillera Bética. Esta formación presenta una gran uniformidad de facies en amplios sectores de la cordillera y tiene su equivalente, en facies y en edad, en la Formación Scaglia Rossa de los Apeninos del centro de Italia y de los Alpes meridionales y en formaciones igualmente análogas en otros dominios alpinos mediterráneos. A

Biodegradation of Garden Waste, Market Waste Using Eisenia fetida and Eudrilus eugenia and Assessment of Manure Quality on Tomato

Science.gov (United States)

Mohan, S. Mariraj

2014-06-01

Comparative study was performed to evaluate the vermicomposting efficiency of two earthworm species Eisenia fetida, Eudrilus eugenia from the garden wastes, vegetable market wastes. Three different experimental works were conducted. For each experiment three plastic vermibins were used. Experiment (1) mentioned for control without earthworms. Experiment (2) bedded with Eudrilus eugenia, Experiment (3) comprised of bedding with Eisenia fetida. Pre composting was allowed for 10 days after that Eudrilus eugenia, Eisenia fetida were added in respective vermibins. The multiplication of earthworms in terms of number was calculated at the end of vermicomposting. The N, P, K value of the manure in each vermibin was estimated before and after the completion of the experiment. High N, P, K value was obtained in Experiment (2) and Experiment (3) compared to control. Among the solid wastes, the vegetable wastes were degraded quickly by Eudrilus eugenia and also it has the best quality of manure. Eudrilus eugenia was found to be efficient for quick degradation of both garden wastes and vegetable wastes. After manure production, field trials were conducted using different fertilizers to assess the manure quality in the growth and yield of tomato plants. Six types of experimental trial pots were prepared where one was kept as control and five others were treated with different category of fertilizers. The treatment pots (P3) showed better growth parameters (leaf numbers, stem diameter, plant height) than the rest of the trial.

Avoidance of Ag nanoparticles by earthworms, Eisenia fetida

DEFF Research Database (Denmark)

Mariyadas, Jennifer; Mónica, Amorim; Scott-Fordsmand, Janeck James

2013-01-01

Earthworms are key sentinel organisms playing an important role in improving the soil structure. Here we tested the avoidance behaviour of earthworms, Eisenia fetida to silver nanoparticles (Ag NPs). Silver nanoparticles are widely used in a range of consumer products mainly as antibacterial agents....... The avoidance behaviour could not be explained by the release of silver ions in the soil-solution. Although, Ag-ions release (if any) may still have had an influence on behaviour. The present results suggests that the earthworms perceive the presence of actual nanoparticles in the soil. Our results suggest that...... and thus causes potential risk to the environment once these particles are released into the environment [1]. In our tests, we were able to show that the earthworms avoided commercially fabricated silver nanoparticles in a dose and time dependent manner. The earthworms were exposed to 3 nanoparticles: NM...

Nota [N] Roja la vibrante historia de un género y una nueva manera de informar

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Blasco Fernando Checa Montúfar

2010-06-01

Full Text Available Presenta algunas reflexiones sobre el libro Nota[N]Roja, señala que se constituye en una guía mínima para periodistas de seguridad pública y justicia. En el se conceptualizan temas como absolución, acusación penal, daño moral querella, menores infractores, se aclaran y desarrollan temas de periodismo como autorregulación, cláusula de conciencia, derecho a la réplica, noticiabilidad, verificación de la información, etc.

Both released silver ions and particulate Ag contribute to the toxicity of AgNPs to earthworm Eisenia fetida

NARCIS (Netherlands)

Li, L.; , van, Gestel C.A.M.

2015-01-01

To disentangle the contribution of ionic and nanoparticulate Ag to the overall toxicity to the earthworm Eisenia fetida, a semi-permeable membrane strategy was used to separate Ag+ released from silver nanoparticles (AgNPs) in an aqueous exposure. Internal Ag fractionation, activities of antioxidant

Use of Avoidance Tests for Investigating Potential of the Earthworm Eisenia fetida to Improve Composting of Grass Clippings

NARCIS (Netherlands)

Illmer, P.; Liebensteiner, M.

2011-01-01

The earthworm Eisenia fetida is the most commonly used worm for worm-supported composting of organic residues. Within the present study, the potential of E. fetida for decomposing grass clippings, an organic waste which usually causes anoxic conditions and thus insufficient degradation in the course

Vides de Simón de Rojas Clemente (1777-1827 en el herbario del Real Colegio Alfonso XII de San Lorenzo de El Escorial (Madrid, España

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Perea, Domingo

1997-12-01

Full Text Available In the present paper, the discovery of grape-vine mounted sheets from the Simón de Rojas Clemente herbarium, which were found in the Herbarium of the Real Colegio Alfonso XH in San Lorenzo de El Escorial, is reported. This material completes the collection of Clemente's vines which makes up one of the historical collections deposited in the MA herbarium.Damos a conocer unos pliegos de vides procedentes del herbario de Simón de Rojas Clemente que han aparecido en el Gabinete de Ciencias Naturales del Real Colegio Alfonso XII de San Lorenzo de El Escorial. Estos materiales completan la colección de vides de Clemente que constituye uno de los herbarios históricos depositados en MA.

The effect of dibenzo-p-dioxin- and dibenzofuran-contaminated soil on the earthworm Eisenia andrei

Czech Academy of Sciences Publication Activity Database

Roubalová, Radka; Dvořák, Jiří; Procházková, Petra; Elhottová, Dana; Rossmann, Pavel; Škanta, František; Bilej, Martin

2014-01-01

Roč. 193, OCT 2014 (2014), s. 22-28 ISSN 0269-7491 R&D Projects: GA MŠk(CZ) EE2.3.20.0055; GA MŠk(CZ) EE2.3.30.0003; GA MŠk(CZ) ED1.1.00/02.0109 Institutional support: RVO:61388971 ; RVO:60077344 Keywords : Eisenia and rei * PCDD/Fs * Calreticulin Subject RIV: EE - Microbiology, Virology Impact factor: 4.143, year: 2014

Toxicity of RDX, HMX, TNB, 2,4-DNT, and 2,6-DNT to the Earthworm, Eisenia Fetida, in a Sandy Loam Soil

National Research Council Canada - National Science Library

Simini, Michael; Checkai, Ronald T; Kuperman, Roman G; Phillips, Carlton T; Kolakowski, Jan E; Kurnas, Carl W; Sunahara, Geoffrey I

2006-01-01

...) for ecological risk assessment of soil contaminants at Superfund sites. Insufficient information existed to generate Eco-SSLs for explosives and related materials in soil. The earthworm (Eisenia fetida...

Toxic responses of Sox2 gene in the regeneration of the earthworm Eisenia foetida exposed to Retnoic acid.

Science.gov (United States)

Tao, Jing; Rong, Wei; Diao, Xiaoping; Zhou, Hailong

2018-01-01

Exogenous retinoic acid delays and disturbs the regeneration of Eisenia foetida. The stem cell pluripotency factor, Sox2, can play a crucial role in cell reprogramming and dedifferentiation. In this study, we compared the regeneration of Eisenia foetida in different segments after amputation and the effects of retinoic acid on the regeneration of different segments. The results showed that the regeneration speed of the head and tail was slightly faster than the middle part, and retinoic acid disrupted and delayed the regeneration of the earthworm. The qRT-PCR and Western blot analysis showed that the expression of the Sox2 gene and Sox2 protein was highest on the seventh day in different segments (pregeneration of earthworms and the formation of blastema are related to the expression of the Sox2 gene and protein. Retinoic acid delays and interferes with the regeneration of the earthworm by affecting the expression levels of the Sox2 gene and protein. Copyright © 2017 Elsevier Inc. All rights reserved.

Gene expression program of regeneration in Eisenia fetida: a transcriptomics study

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Aksheev Bhambri

2017-10-01

Full Text Available Annelids form a connecting link between segmented and non-segmented organisms.  In other words, phylogenetically, the segmented body pattern starts from Annelida, a phylum that consists of thousands of species, including marine worms, freshwater leeches and earthworms that inhabit deep layers of soil to environmental niches in forests and cultivated land. We are using Eisenia fetida (Indian isolate a top dwelling, vermicomposting worm due to its ability to regenerate its posterior after damage, injury or complete removal. On average, Eisenia fetida has 100-110 segments. We separated the anterior (upto 55-60th segment and posterior of the worm, and allowed it to regenerate.  In this model, only the posterior could be regenerated after injury.  We isolated RNA from the regenerated tissue and the immediate adjacent old tissue at 15 days, 20 days and 30 days during regeneration. We carried out transcriptome sequencing and analysis. With the aim of identifying specific factors which promote nerve regeneration, we have annotated the differentially expressed genes. In all organisms which possess a segmented body, the expression pattern of the Hox cluster is conserved. Hox gene expression, a conserved developmental phenomenon in establishment of body plan has been studied by comparative genomics of other annelids like the marine worm Capitella telleta, the leech Helobdella robusta.  We have used a combination of high-throughput sequencing based techniques and validation through cell and molecular biology to identify key aspects of the gene expression program of regeneration in this worm. Besides the transcriptome, we have also done whole genome sequencing, miRnome and metagenome sequencing of this terrestrial annelid.

Aplasia pura de serie roja post-trasplante alogeneico de células progenitoras hematopoyeticas ABO incompatible Pure red cell aplasia after ABO incompatible bone marrow transplantation

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E. Bulliorsky

2002-12-01

Full Text Available El trasplante alogeneico de células progenitoras hematopoyéticas (TCPH con incompatibilidad ABO entre el donante y el receptor puede en ocasiones asociarse a trastornos en la progenie eritroide desarrollada a partir de la médula ósea trasplantada, caracterizado por un funcionamiento tardío, inadecuado e incompleto de la misma. En este contexto, la aplasia pura de serie roja es la complicación más severa. Se han intentado tratamientos para la aplasia pura de serie roja post-TCPH con eritropoyetina o plasmaféresis, con relativo éxito. Algunos autores han informado también la utilización de globulina antilinfocitaria, asumiendo que dicha aplasia selectiva de la serie roja en la médula ósea trasplantada es mediada por un mecanismo inmune. En este trabajo se describe un paciente portador de una leucemia aguda en quien se realizó un TCPH alogeneico (ABO incompatible con su donante. Teniendo niveles bajos de aglutininas contra el grupo sanguíneo de la donante, desarrolló una aplasia pura de serie roja post - TCPH. La misma no mejoró con tratamiento con eritropoyetina o con un refuerzo de progenitores hematopoyéticos de sangre periférica de la misma donante (boost, resolviéndose totalmente luego de un tratamiento exitoso con globulina antilinfocitaria de origen equino.ABO incompatibility in allogeneic bone marrow transplantation may be associated with incomplete or delayed erythroid engraftment, being pure red cell aplasia (PRCA the most severe complication in this setting. Attempts for the treatment of PRCA have been made with erythropoietin or with plasmapheresis with relative success, and some authors have reported the reversibility of PRCA with antilymphocyte globulin (ALG or ATG, based on the assumption that PRCA might be immunologically mediated. We report herewith a patient with acute leukemia who developed post - BMT pure red cell aplasia. His sibling donor (sister was HLA identical and ABO incompatible, having low agglutinin

The Power of the Dramatic «Auctoritas»: Francisco de Rojas Zorrilla in front of the Byzantine Comedy

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Julián González-Barrera

2018-01-01

Full Text Available Francisco de Rojas Zorrilla was one of the poets most favoured by the Court, even more than Calderón de la Barca. That royal favour gave him a creative freedom that lead to experiment with new methods, genres and motifs. That was the case of Persiles and Sigismunda, not only a mere adaptation of the cervantine novel, but also an attempt to surpass the model that Lope de Vega had created for the byzantine comedy, adding new formulas to a genre considered closed.

Experimentación dramatúrgica en Rojas Zorrilla: alternancia y simultaneidad espacial en Los trabajos de Tobías

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Rubiera Fernández, Javier

2007-06-01

Full Text Available This article aims to analyse the technique used by Rojas Zorilla in a religious comedy of biblical derivation to represent, in a parallel way, dramatic actions that are simultaneously developed in very separate dramatic spaces. Although basing his approach on ancient stage procedures, by combining these with modern techniques the result is of such originality that we consider it to be «experimentation». In order to properly value his creative method, a close comparison is made between the biblical narrative, a sixteenth century auto, and a comedy by Lope de Vega that address the same subject.El objetivo de este artículo es analizar la técnica empleada por Rojas Zorrilla, en una comedia religiosa de fuente bíblica, para representar de modo paralelo acciones dramáticas que se desarrollan simultáneamente en espacios dramáticos muy alejados. A pesar de basarse en procedimientos escénicos antiguos, al combinarlo con técnicas modernas el resultado es de tal originalidad que lo calificamos de «experimentación». Para valorar debidamente su modo de componer, se hace una estrecha comparación con el relato bíblico, con un auto del siglo XVI y con una comedia de Lope de Vega que tratan el mismo tema.

Obtaining of transgenic papaya plants var. Maradol roja that carry out the rice oryzacystatin gene

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Milady F. Mendoza

2004-10-01

Full Text Available Papaya (Carica papaya L., is severely affected by Papaya Ringspot virus, which belongs to plant potyvirus group. A recent strategy for pest control produced by this virus is the transformation with genes encoding cysteine proteinase inhibitors. Rice oryzacistatin gene encoding for cystatins, was inserted in a pCAMBIA binary vector, for genetic transformation of papaya somatic embryos var. Maradol roja, mediated by gene gun. Gene integration was confirmed by means of polimerase chain reaction using the primers designed from gene bar sequence. Forty out of eighty in vitro transgenic papaya lines amplified a 402 fragment which correspond to the expecting size. Key words: Carica papaya, genetic engineering, potyvirus, proteinase inhibitor

Recycling of organic wastes by employing Eisenia fetida.

Science.gov (United States)

Yadav, Anoop; Garg, V K

2011-02-01

This paper reports the recycling of nutrients by vermicomposting of cow dung (CD), poultry droppings (PD) and food industry sludge (FIS) employing earthworms (Eisenia fetida). A total of six vermicomposting units were established and dynamics of chemical and biological parameters has been studied for 13 weeks. The waste mixture containing 50% CD+25% PD+25% FIS had better fertilizer value among studied waste combinations. At the end of experiment, vermicomposts showed decrease in pH and organic C, but increase in EC, total Kjeldhal N, total available P and total K contents. The C:N ratio of final vermicomposts also reduced to 10.7-12.7 from 22.8 to 56 in different waste combinations. The earthworms have good biomass gain and cocoon production in all vermicomposting units but CD alone and 50% CD+25% PD+25% FIS were better than other studied combinations. Copyright © 2010 Elsevier Ltd. All rights reserved.

Ecotoxicological assessment of TiO{sub 2} byproducts on the earthworm Eisenia fetida

Energy Technology Data Exchange (ETDEWEB)

Bigorgne, Emilie, E-mail: emilie.bigorgne@umail.univ-metz.fr [Laboratoire Interactions Ecotoxicite, Biodiversite, Ecosystemes, Universite Paul Verlaine - Metz, CNRS UMR 7146, Rue du General Delestraint, 57070 Metz (France); Foucaud, Laurent [Laboratoire Interactions Ecotoxicite, Biodiversite, Ecosystemes, Universite Paul Verlaine - Metz, CNRS UMR 7146, Rue du General Delestraint, 57070 Metz (France); Lapied, Emmanuel [Bioforsk, Soil and Environment, Fredrik A. Dahls vei 20, N-1432 Aas (Norway); Labille, Jerome; Botta, Celine [CEREGE UMR 6635 CNRS/Aix-Marseille Universite, Europole de l' Arbois, 13545 Aix-en-Provence (France); International Consortium for the Environmental Implications of Nanotechnology iCEINT, Europole de l' Arbois, 13545 Aix en Provence (France); Sirguey, Catherine [Nancy Universite, INPL/INRA, UMR 1120, Laboratoire Sols et Environnement, BP 172-2, Avenue de la foret de Haye, F-54505 Vandoeuvre-les-Nancy Cedex (France); Falla, Jairo [Laboratoire Interactions Ecotoxicite, Biodiversite, Ecosystemes, Universite Paul Verlaine - Metz, CNRS UMR 7146, Rue du General Delestraint, 57070 Metz (France); Rose, Jerome [CEREGE UMR 6635 CNRS/Aix-Marseille Universite, Europole de l' Arbois, 13545 Aix-en-Provence (France); International Consortium for the Environmental Implications of Nanotechnology iCEINT, Europole de l' Arbois, 13545 Aix en Provence (France); Joner, Erik J. [Nancy Universite, INPL/INRA, UMR 1120, Laboratoire Sols et Environnement, BP 172-2, Avenue de la foret de Haye, F-54505 Vandoeuvre-les-Nancy Cedex (France); Rodius, Francois; Nahmani, Johanne [Laboratoire Interactions Ecotoxicite, Biodiversite, Ecosystemes, Universite Paul Verlaine - Metz, CNRS UMR 7146, Rue du General Delestraint, 57070 Metz (France)

2011-10-15

The increasing production of nanomaterials will in turn increase the release of nanosized byproducts to the environment. The aim of this study was to evaluate the behaviour, uptake and ecotoxicity of TiO{sub 2} byproducts in the earthworm Eisenia fetida. Worms were exposed to suspensions containing 0.1, 1 and 10 mg/L of byproducts for 24 h. Size of TiO{sub 2} byproducts showed aggregation of particles up to 700 {mu}m with laser diffraction. Only worms exposed at 10 mg/L showed bioaccumulation of titanium (ICP-AES), increasing expression of metallothionein and superoxide dismutase mRNA (Real-time PCR) and induction of apoptotic activity (Apostain and TUNEL). TiO{sub 2} byproducts did not induce cytotoxicity on coelomocytes, but a significant decrease of phagocytosis was observed starting from 0.1 mg/L. In conclusion, bioaccumulation of byproducts and their production of reactive oxygen species could be responsible for the alteration of the antioxidant system in worms. - Highlights: > Aggregation of TiO{sub 2} byproducts up to 700 {mu}m in the medium of exposure. > Bioaccumulation of titanium in worms exposed at 10 mg/L of TiO{sub 2} byproducts. > Increasing expression of metallothionein and superoxide dismutase mRNA. > Induction of apoptotic activity in worms exposed at 10 mg/L of TiO{sub 2} byproducts. > Decrease of coelomocytes phagocytosis starting from 0.1 mg/L of TiO{sub 2} byproducts. - A short time exposure to TiO{sub 2} byproducts can induce sublethal effects on the earthworm, Eisenia fetida.

Correlation between the activity of digestive enzymes and nonself recognition in the gut of Eisenia andrei earthworms

Czech Academy of Sciences Publication Activity Database

Procházková, Petra; Šustr, Vladimír; Dvořák, Jiří; Roubalová, Radka; Škanta, František; Pižl, Václav; Bilej, Martin

2013-01-01

Roč. 114, č. 3 (2013), s. 217-221 ISSN 0022-2011 R&D Projects: GA MŠk(CZ) EE2.3.20.0055; GA MŠk(CZ) EE2.3.30.0003; GA ČR GA206/07/0378 Institutional support: RVO:61388971 ; RVO:60077344 Keywords : Digestive enzyme * Earthworm * Eisenia andrei Subject RIV: EC - Immunology; EE - Microbiology, Virology (BC-A) Impact factor: 2.601, year: 2013

PARÁMETROS GENÉTICOS PARA CRECIMIENTO COMERCIAL SOBREVIVENCIA Y MANCHADO EN TILAPIA ROJA (Oreochromis sp. EN COLOMBIA

Directory of Open Access Journals (Sweden)

C. E. Pulgarin

2012-01-01

Full Text Available El objetivo del presente trabajo fue estimar los efectos genéticos para peso al momento de sexaje, crecimiento y sobrevivencia hasta cosecha, y proporción de mancha (área de mancha y presencia/ausencia de mancha durante la fase comercial en un grupo de 86 familias de hermanos enteros y 31 familias de hermanos medios de tilapia roja ( Oreochromis sp.. La media de peso durante la fase de crecimiento comercial fue de 181,4 g (184 g para machos y 178 g para hembras, los machos significativamente más grandes que las hembras (P < 0,001. Las variables de sobrevivencia, área de mancha y ausencia de mancha no presentaron diferencias entre los sexos. Las heredabilidades estimadas ( h 2 ± e . s para crecimiento comercial y sobrevivencia fueron 0,23±0,02 y 0,05±0,03, respectivamente. e l porcentaje de mancha y la ausencia y presencia de la misma mostraron heredabilidades cercanas a cero, lo cual indica que estos dos caracteres están relacionados más con efectos ambientales que con efectos genéticos aditivos. s e encontró una correlación favorable y significativa entre sobrevivencia y crecimiento comercial (0,24; P < 0,05. Los resultados de este trabajo indican que mediante la explotación de la genética aditiva es posible mejorar el desempeño de los animales para crecimiento comercial y sobrevivencia en la tilapia roja, mientras que poco progreso se puede esperar por medio de la selección para variables relacionadas con el manchado corporal en la población evaluada.

Pathogen reduction in septic tank sludge through vermicomposting using Eisenia fetida.

Science.gov (United States)

Rodríguez-Canché, L G; Cardoso Vigueros, L; Maldonado-Montiel, T; Martínez-Sanmiguel, M

2010-05-01

This study evaluated the potential of earthworms (Eisenia fetida) to remove pathogens from the sludge from septic tanks. Three earthworm population densities, equivalent to 1, 2, and 2.5kgm(-2), were tested for pathogen removal from sludge. The experimental phase lasted 60days, starting from the initial earthworm inoculation. After 60days, it was found that earthworms reduced concentrations of fecal coliforms, Salmonella spp., and helminth ova to permissible levels (<1000MPN/g, <3MPN/g, and <1viable ova/g on a dry weight basis, respectively) in accordance with Official Mexican Standard of environmental protection (NOM-004-SEMARNAT-2002) (SEMARNAT, 2002). Thus, sludge treatment with earthworms generated Class A biosolids, useful for forest, agricultural, and soil improvement. Copyright 2009 Elsevier Ltd. All rights reserved.

Possibilities of using Eisenia Andrei earthworms as bioindicators of radioactive soil contamination

International Nuclear Information System (INIS)

Galanda, D.; Strisovska, J.; Lacenova, A.

2017-01-01

Eisenia Andrei is known under various generic names such as Californian daisy, red daisy, tiger worm etc. It is a species of earthworm that is physiologically adapted to life in a medium containing organic matter. Staining (vermiremedia technology) of soils contaminated with heavy metals belongs among innovative and efficient techniques. According to published works, this technology is easy to apply. Dandelions are used for soil rehabilitation, rejuvenation and mineral enrichment in the soil. In the soil environment, the effect of the sunsets is known as the drilosphere of the system, that is, the soil is under their direct influence. The aim of the present paper was to verify the possibility of application and subsequent use of Eisenia Andrei sunflowers as bioindicators in soil contaminated by radionuclides "2"3"9Pu and "2"4"1Am, which are significant anthropogenic radionuclides emitting alpha particles. Another circle of radionuclides were anthropogenic radionuclides "6"0Co and "1"3"7Cs emitting gamma rays. Based on the radionuclides used, two groups of target-contaminated samples were subjected to experimentation and spectrometric analysis alone. The first group of experimental subjects was made up of earthworms living in alpha radionuclide-enriched soils, and in the second group of gamma-radionuclides. Radiochemical analysis of alpha radionuclides was performed by a separation method of extraction chromatography using commercial sorbents from Triskem-TEVA Resin and DGA Resin. The preparation of specimens for the spectroscopic analysis of the emitted alpha configuration was performed by co-precipitation with NdCl_3. The measurement itself was performed with the ORTEC 576A Alpha Spectrometer with the GammaVision software. The activity of gamma radionuclides in a sample of animals devoid of possible surface contamination from the nutrient medium of the contaminated soil was measured using the Ortec gamma spectrometer. (authors)

Metabolic responses of Eisenia fetida after sub-lethal exposure to organic contaminants with different toxic modes of action

Energy Technology Data Exchange (ETDEWEB)

McKelvie, Jennifer R.; Wolfe, David M.; Celejewski, Magda A. [Department of Physical and Environmental Sciences, University of Toronto, 1265 Military Trail Toronto, ON M1C 1A4 (Canada); Alaee, Mehran [Environment Canada, 867 Lakeshore Rd., P.O. Box 5050, Burlington, ON L7R 4A6 (Canada); Simpson, Andre J. [Department of Physical and Environmental Sciences, University of Toronto, 1265 Military Trail Toronto, ON M1C 1A4 (Canada); Simpson, Myrna J., E-mail: myrna.simpson@utoronto.ca [Department of Physical and Environmental Sciences, University of Toronto, 1265 Military Trail Toronto, ON M1C 1A4 (Canada)

2011-12-15

Nuclear magnetic resonance (NMR) - based metabolomics has the potential to identify toxic responses of contaminants within a mixture in contaminated soil. This study evaluated the metabolic response of Eisenia fetida after exposure to an array of organic compounds to determine whether contaminant-specific responses could be identified. The compounds investigated in contact tests included: two pesticides (carbaryl and chlorpyrifos), three pharmaceuticals (carbamazephine, estrone and caffeine), two persistent organohalogens (Aroclor 1254 and PBDE 209) and two industrial compounds (nonylphenol and dimethyl phthalate). Control and contaminant-exposed metabolic profiles were distinguished using principal component analysis and potential contaminant-specific biomarkers of exposure were found for several contaminants. These results suggest that NMR-based metabolomics offers considerable promise for differentiating between the different toxic modes of action (MOA) associated with sub-lethal toxicity to earthworms. - Highlights: > NMR-based earthworm metabolomic analysis of the toxic mode of action of various environmental contaminants. > Organic chemicals with different toxic modes of action resulted in varied metabolomic responses for E. fetida. > NMR-based metabolomics differentiates between the different modes of action associated with sub-lethal toxicity. - {sup 1}H NMR metabolomics was used to identify potential biomarkers of organic contaminant exposure in Eisenia fetida earthworms.

Soil type influence on Ag Nanoparticles by earthworms, Eisenia fetida

DEFF Research Database (Denmark)

Mariyadas, Jennifer; Mónica, Amorim; Scott-Fordsmand, Janeck James

2014-01-01

Earthworms are key sentinel organisms playing an important role in improving the soil structure. Here we tested the importance of soil type on the toxicity to silver nanoparticles (Ag NPs) to earthworms, Eisenia fetida. Silver nanoparticles are widely used in a range of consumer products mainly...... as antibacterial agents and thus causes potential risk to the environment once these particles are released into the environment [1]. In our tests, we were able to show that the earthworm toxicity was strongly dependent on the soil type, with strongest effect in low organic matter soil. Studies on the organic...... matter content, clay and cation exchange capacity along with the metal solution activity will provide insight into the bioavailability of metals in different soils, hence For each of the soil type the fate of the AgNPs was also measured....

Acute and chronic toxicity testing of TPH-contaminated soils with the earthworm, Eisenia foetida

International Nuclear Information System (INIS)

Stewart, A.J.; Wicker, L.F.; Nazerias, M.S.

1995-01-01

Responses of Eisenia foetida to petroleum-contaminated soils are being assessed using a 21-day test described previously. The authors prepared dilutions of two soils, referred to as A and B, using their reference-soil counterparts, collected from near the contaminated sites. The total petroleum hydrocarbon (TPH) content of each soil was measured by latroscan before the dilutions were prepared. References for the A and B soils contained 167 and 1,869 ppm of TPH, respectively. Thus, neither reference soil was pristine. Dilutions of the A soil tested with E. foetida contained from 179 to 305 ppm TPH; dilutions of the B soil contained from 1,875 to 1,950 ppm TPH. E foetida survival was 100% in both dilution series. Mean growth of Eisenia in dilutions of the A soil ranged from 48 to 74 mg dry-weight growth per pair of worms; these values were lower than those in any dilution of the B soil series. Lipid levels of worms in higher concentrations of the A and B soils were similar to one another and to published values, suggesting little inhibition of feeding in either dilution series. Earthworm reproduction was zero in the A series, but moderately high in the B series. Thus, the A soil apparently contained materials other than TPH that inhibited earthworm growth and reproduction. This study shows that (1) TPH at concentrations as high as 1,800 ppm may not always be inhibitor to earthworm growth or reproduction and (2) that earthworm survival, as a test endpoint, is much less sensitive than either growth or reproduction

Comparison of techniques for estimating PAH bioavailability: Uptake in Eisenia fetida, passive samplers and leaching using various solvents and additives

International Nuclear Information System (INIS)

Bergknut, Magnus; Sehlin, Emma; Lundstedt, Staffan; Andersson, Patrik L.; Haglund, Peter; Tysklind, Mats

2007-01-01

The aim of this study was to evaluate different techniques for assessing the availability of polycyclic aromatic hydrocarbons (PAHs) in soil. This was done by comparing the amounts (total and relative) taken up by the earthworm Eisenia fetida with the amounts extracted by solid-phase microextraction (SPME), semi-permeable membrane devices (SPMDs), leaching with various solvent mixtures, leaching using additives, and sequential leaching. Bioconcentration factors of PAHs in the earthworms based on equilibrium partitioning theory resulted in poor correlations to observed values. This was most notable for PAHs with high concentrations in the studied soil. Evaluation by principal component analysis (PCA) showed distinct differences between the evaluated techniques and, generally, there were larger proportions of carcinogenic PAHs (4-6 fused rings) in the earthworms. These results suggest that it may be difficult to develop a chemical method that is capable of mimicking biological uptake, and thus estimating the bioavailability of PAHs. - The total and relative amounts of PAHs extracted by abiotic techniques for assessing the bioavailability of PAHs was found to differ from the amounts taken up by Eisenia fetida

Comparison of techniques for estimating PAH bioavailability: Uptake in Eisenia fetida, passive samplers and leaching using various solvents and additives

Energy Technology Data Exchange (ETDEWEB)

Bergknut, Magnus [Department of Chemistry, Environmental Chemistry, Umeaa University, SE-90187 Umeaa (Sweden)]. E-mail: magnus.bergknut@chem.umu.se; Sehlin, Emma [Department of Chemistry, Environmental Chemistry, Umeaa University, SE-90187 Umeaa (Sweden); Lundstedt, Staffan [Department of Chemistry, Environmental Chemistry, Umeaa University, SE-90187 Umeaa (Sweden); Andersson, Patrik L. [Department of Chemistry, Environmental Chemistry, Umeaa University, SE-90187 Umeaa (Sweden); Haglund, Peter [Department of Chemistry, Environmental Chemistry, Umeaa University, SE-90187 Umeaa (Sweden); Tysklind, Mats [Department of Chemistry, Environmental Chemistry, Umeaa University, SE-90187 Umeaa (Sweden)

2007-01-15

The aim of this study was to evaluate different techniques for assessing the availability of polycyclic aromatic hydrocarbons (PAHs) in soil. This was done by comparing the amounts (total and relative) taken up by the earthworm Eisenia fetida with the amounts extracted by solid-phase microextraction (SPME), semi-permeable membrane devices (SPMDs), leaching with various solvent mixtures, leaching using additives, and sequential leaching. Bioconcentration factors of PAHs in the earthworms based on equilibrium partitioning theory resulted in poor correlations to observed values. This was most notable for PAHs with high concentrations in the studied soil. Evaluation by principal component analysis (PCA) showed distinct differences between the evaluated techniques and, generally, there were larger proportions of carcinogenic PAHs (4-6 fused rings) in the earthworms. These results suggest that it may be difficult to develop a chemical method that is capable of mimicking biological uptake, and thus estimating the bioavailability of PAHs. - The total and relative amounts of PAHs extracted by abiotic techniques for assessing the bioavailability of PAHs was found to differ from the amounts taken up by Eisenia fetida.

Mareas rojas de Mesodinium rubrum (Lohmann) Hamburger y Buddenbrock en el Golfo de California (Invierno de 1998)

OpenAIRE

Gárate-Lizárraga, Ismael; Band-Schmidt, Christine; Cervantes-Duarte, Rafael; Escobedo-Urías, Diana

2002-01-01

Durante el invierno de 1998 se colectaron un total de 13 muestras de mareas rojas causadas por el protozoario fotosintético Mesodinium rubrum en el Golfo de California. El registro de temperatura del agua varió de 20.3 a 23.2°C. La salinidad, el oxígeno disuelto, el pH, y la concentración de nutrientes se determinaron sólo en los parches observados en las costas de Sinaloa. La salinidad varió entre 34.71 y 35.25. El oxígeno disuelto osciló entre 5.29 y 6.78 ml/l y el pH varió de 8.16 a 8.27. ...

Management of fresh water weeds (macrophytes) by vermicomposting using Eisenia fetida.

Science.gov (United States)

Najar, Ishtiyaq Ahmed; Khan, Anisa B

2013-09-01

In the present study, potential of Eisenia fetida to recycle the different types of fresh water weeds (macrophytes) used as substrate in different reactors (Azolla pinnata reactor, Trapa natans reactor, Ceratophyllum demersum reactor, free-floating macrophytes mixture reactor, and submerged macrophytes mixture reactor) during 2 months experiment is investigated. E. fetida showed significant variation in number and weight among the reactors and during the different fortnights (P macrophytes mixture reactor (number 105 ± 5.77 %; weight 41.07 ± 3.97 % ). ANOVA showed significant variation in cocoon production (F4 = 15.67, P macrophyte affects the growth and reproduction pattern of E. fetida among the different reactors, further the addition of A. pinnata in other macrophytes reactors can improve their recycling by E. fetida.

Potential utilization of bagasse as feed material for earthworm Eisenia fetida and production of vermicompost

OpenAIRE

Bhat, Sartaj Ahmad; Singh, Jaswinder; Vig, Adarsh Pal

2015-01-01

In the present work bagasse (B) i.e waste of the sugar industry, was fed to Eisenia fetida with cattle dung (CD) support as feed material at various ratios (waste: CD) of 0:100 (B0), 25:75 (B25), 50:50 (B50), 75:25 (B75) and 100:0 (B100) on dry weight basis. Co-composting with cattle dung helped to improve their acceptability for E. fetida and also improved physico-chemical characteristics. Best appropriate ratio for survival, maximum growth and population buildup of E. fetida was determined ...

Efecto del extracto metanólico y las fracciones acuosa y butanólica de Lepidium meyenii variedad roja sobre la función renal en ratas Sprague-Dawley macho tratadas durante 90 días

OpenAIRE

Bernuy Hurtado, Jeanette Marlene

2017-01-01

Introducción: La fracción n-butanólica del extracto metanólico de maca contiene alcaloides que se ha sugerido pueden ser tóxicos. Objetivo: conocer el efecto de la administración del extracto metanólico y la fracción acuosa y butanólica de maca roja por vía orogástrica sobre la función renal de ratas Sprague-Dawley macho durante 90 días. Métodos: Se distribuyeron en: Grupo 1: control (agua destilada), Grupo 2: (1g de extracto metanólico de maca roja/k/día), Grupo 3: (0.89gr de fracción acuosa...

PRECOMPOSTEO DE RESIDUOS ORGÁNICOS Y SU EFECTO EN LA DINÁMICA POBLACIONAL DE Einsenia foetida

Directory of Open Access Journals (Sweden)

Carlos Manuel Acosta-Durán

2013-01-01

Full Text Available El compostaje y el vermicompostaje son técnicas que se utilizan para transformar los residuos sólidos orgánicos en abonos orgánicos (composta y vermicomposta, respectivamente cuyas características físicas, químicas y biológicas inciden directamente en el mejoramiento del suelo y en el crecimiento de las plantas; sin embargo, durante el proceso, se liberan desechos que agreden al ambiente. El sustrato utilizado para alimentar a las lombrices debe pasar por un periodo previo de compostaje, conocido como precompostaje. El vermicompostaje produce un material en el que la mayoría de los nutrimentos se encuentran en mayor disponibilidad para la planta, comparados con el material resultante de un proceso de compostaje convencional. El pre compostaje involucra un mayor tiempo y gasto de insumos, lo que puede incrementar el costo de la vermicomposta, por lo que es necesario establecer el tiempo necesario de precompostaje para que los residuos orgánicos puedan emplearse como sustrato en la producción de la lombriz Eisenia spp. Para la obtención de vermicom posta, los residuos se sometieron a un procesode precompostaje de 0 a 8 semanas antes delproceso de vermicompostaje. Se concluyó que las condiciones en las que se desarrolló el experimento permitieron el desarrollo de las lombrices en todos los periodos de precompostaje. El vermicompostaje en capas reduce significativamente el tiempo del proceso para lograr la completa descomposición de los residuos orgánicos. Para la presencia de cocones y juveniles no es necesario el precomposteo. En general el precomposteo de 0 a 2 semanas favorece la reproducción y el de 3 a 7 semanas favorece el crecimiento individual de las lombrices. El mejor tratamiento fue el pre composteo de 2 semanas, debido a que favorece la reproducción y el aumento del peso promediode la lombriz. El precompostaje no es necesario cuando las pilas de vermicomposteo tienenmenos de 50 cm de altura, al producir vermicomposta

Die metaboliseerbare energiewaarde van erdwurmmeel (Eisenia fetida, Oligochaeta as potensiële proteïenbron vir dierevoeding

Directory of Open Access Journals (Sweden)

J. N. Alberts

1988-03-01

Full Text Available Die komposwurm, Eisenia fetida, kan op groot skaal op organiese afval geteel word. Dit is dus potensieel moontlik om dierlike afvalproteiene te herwin indien E. fetida as proteienbron in gebalanseerde veerantsoene aangewend kan word. Daarom is dieproteienwaarde van die erdwurmspesie ondersoek deur die metaboliseerbare energiewaarde te bepaal. WME-waardes was relatief hoog vir erdwurmmeel, nl. 15,51 en 15,3 MJ kg-1. Dit stem goed ooreen met die van vismeel en karkasmeel.

Analysis of Waste in the Production of Flour California Red Worm (eisenia foet) in Manabí Technical Universitypilot Plant

OpenAIRE

Ulbio Alcívar-Cedeño; Alex Dueñas-Rivadeneira; Eli Sacon-Vera; Gretel Villanueva-Ramos; Luis Bravo-Sánchez

2016-01-01

The necessity for have efficient tools in the environmental assessment of production processesis treated in this paper. This work is related to a previous work made in the Technical University of Manabi, forprotein supplements production from unconventional raw materials, specifically Earthworm (Eisenia foetida)flour, using various ecotoxicological methods to evaluategeneratedwaste in pilot production, in order to contribute to compliance the environmental regulations.Liquid wastes generated ...

Metabolic responses of Eisenia fetida after sub-lethal exposure to organic contaminants with different toxic modes of action

International Nuclear Information System (INIS)

McKelvie, Jennifer R.; Wolfe, David M.; Celejewski, Magda A.; Alaee, Mehran; Simpson, Andre J.; Simpson, Myrna J.

2011-01-01

Nuclear magnetic resonance (NMR) - based metabolomics has the potential to identify toxic responses of contaminants within a mixture in contaminated soil. This study evaluated the metabolic response of Eisenia fetida after exposure to an array of organic compounds to determine whether contaminant-specific responses could be identified. The compounds investigated in contact tests included: two pesticides (carbaryl and chlorpyrifos), three pharmaceuticals (carbamazephine, estrone and caffeine), two persistent organohalogens (Aroclor 1254 and PBDE 209) and two industrial compounds (nonylphenol and dimethyl phthalate). Control and contaminant-exposed metabolic profiles were distinguished using principal component analysis and potential contaminant-specific biomarkers of exposure were found for several contaminants. These results suggest that NMR-based metabolomics offers considerable promise for differentiating between the different toxic modes of action (MOA) associated with sub-lethal toxicity to earthworms. - Highlights: → NMR-based earthworm metabolomic analysis of the toxic mode of action of various environmental contaminants. → Organic chemicals with different toxic modes of action resulted in varied metabolomic responses for E. fetida. → NMR-based metabolomics differentiates between the different modes of action associated with sub-lethal toxicity. - 1 H NMR metabolomics was used to identify potential biomarkers of organic contaminant exposure in Eisenia fetida earthworms.

Rapid bioassessment methods for assessing the toxicity of terrestrial waste sites at the Savannah River Site using the earthworm, Eisenia foetida

International Nuclear Information System (INIS)

Specht, W.L.

1995-08-01

Studies were conducted to assess the feasibility of using the earthworm, Eisenia foetida, to evaluate the toxicity of contaminated soils at the Savannah River Site. Survival was assessed in several uncontaminated soils, including sandy loams and clayey loams, as well as in soils contaminated with coal fines, ash, diesel fuel, and heavy metals. In addition, behavior responses, changes in biomass, and bioaccumulation of heavy metals were assessed as sublethal indicators of toxicity. The results indicate excellent survival of Eisenia foetida in uncontaminated sandy and clayey soils. No amendment of these uncontaminated soils or addition of food was necessary to sustain the worms for the 14-day test period. In contaminated soils, no significant mortality was observed, except in soils which have very low pH (< 3). However, sublethal responses were observed in earthworms exposed to several of the contaminated soils. These responses included worms clumping on the surface of the soil, worms clumping between the sides of the test container and the soil, increased burrowing times, reductions in biomass, and elevated concentrations of heavy metals in worm tissue

CARACTERIZACIÓN DE LA FRACCIÓN ORGÁNICA DE HUMUS DE LOMBRIZ Y COMPOSTS PRODUCIDOS A PARTIR DE DIFERENTES SUSTRATOS

Directory of Open Access Journals (Sweden)

Myriam Rocío Melgarejo P

2010-10-01

Full Text Available Para evaluar la calidad y el grado de humificación de diferentes materiales compostados, se caracterizó químicamente la fracción orgánica de humus de lombriz provenientes de desechos de cocina y de huerta, pulpa de café, basuras biodegradables y residuos de rosas, y de composts obtenidos de residuos de rosas y residuos de clavel. Para tal fin se hizo la determinación y análisis de la relación C/N, el fraccionamiento de la materia orgánica, la purificación y caracterización de los ácidos húmicos por análisis elemental C, H, N, O, espectroscopia visible- ultravioleta y se hallaron diferentes parámetros de humificación. El fraccionamiento de la materia orgánica indicó un bajo contenido de carbono extraído con relación a lo encontrado normalmente en el humus de los suelos. El análisis elemental de los ácidos húmicos de los composts y lombricomposts no reveló diferencias importantes entre los materiales, mientras que la relación E4/E6 proporcionó cambios más evidentes. Los resultados indicaron que la relación C/N no fue un indicativo absoluto del estado de madurez de los materiales estudiados. De los parámetros de humificación analizados, la razón de polimerización, el índice de humificación y la relación entre carbono extraído y carbono no extraído resultaron ser los parámetros que mejor estiman la madurez de los composts y lombricomposts. En cuanto a los materiales evaluados, el lorabricompost de residuos de rosas reveló en conjunto las mejores condiciones con relación al contenido y calidad de la materia orgánica para ser adicionado a un suelo.

Effects of Sludge Dry Solid Content and Residual Bulking Agents on Volatile Solids Reduction Using Eisenia foetida

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Mohammad ali Abdoli

2009-06-01

Full Text Available In the first stage of this study, the compound effects of sludge dry solid content and residual bulking agent type (paper, saw dust, straw mixed with activated sludge (10, 15, and 20% dry solids on volatile solids (V.S. reduction were investigated using Eisenia foetida in pilot scale experiments with batches of fifty earthworms in each of the 10 experimental treatments over a period of 10 weeks. The maximum V.S. reduction was attained in the mixture of sludge and paper, with a D.S. of 15% (0.42 ± 0.03 % day-1 while the minimum V.S. reduction was achieved in the mixture of sludge and straw, with a D.S. of 10% (0.26 ± 0.01 % day-1. In the second stage, the survival of Eisenia foetida in the anaerobic sewage sludge was investigated. In the unmixed raw anaerobic sludge, all the earthworms died during the first 9 weeks of the study period due to acute toxicity. From week 10, however, their survival rate improved so that by week 12 when toxicity reduced to 25.40%, they completely survived. This is while in the mixture of anaerobic sludge with paper (D.S. 15%, 100% of the earthworms survived from week 8 after the volatile solids reduced to 20.42% and 17.40%.

Effects of acute gamma radiation on the reproductive ability of the earthworm Eisenia fetida

International Nuclear Information System (INIS)

Sowmithra, K.; Shetty, N.J.; Harini, B.P.; Jha, S.K.; Chaubey, R.C.

2015-01-01

Earthworms are the most suitable biological indicators of radioactive pollution because they are the parts of nutritional webs, and are present in relatively high numbers. Four months old Eisenia fetida were exposed to different doses of gamma radiation, namely 1, 2, 3, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 and 60 Gy to study the effects of radiation on different reproductive parameters. The number of cocoons laid and the hatchlings emerged were recorded for all the selected doses. There was no reduction in cocoon production, however; decreasing size and weight of the cocoons was observed from the samples exposed to 20 Gy and above doses. Significant reductions in the hatchlings were recorded in earthworms exposed to 10 Gy and above doses. The dose response curves for a percentage reduction in hatchlings were constructed. Exposure to radiation dose of 1 and 2 Gy did not show any reduction, however, there was ≈10%, ≈50% and ≈90% decrease in the hatchlings in samples exposed to 3, 15 and 45, 50, 55 and 60 Gy doses respectively. Delayed hatchability was also reported at al exposure level. Histology of irradiated earthworms revealed that the structural damage in the seminal vesicles was prominent at the exposed dose of 3 Gy onwards with complete degeneration on exposure to 60 Gy of gamma radiation. - Highlights: • Eisenia fetida exposed to several doses of gamma-radiation to study the impact on reproduction. • There was no reduction in the cocoon production however. • There was reduction in size, weight and change in shape of the cocoons observed. • Reduction in number of hatchlings and degradation of seminal vesicles was pragmatic

Data on proteins of lysenin family in coelomocytes of Eisenia andrei and E. fetida obtained by tandem mass spectrometry coupled with liquid chromatography

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Bianka Swiderska

2016-12-01

Full Text Available The data described are related to the article “Lysenin family proteins in earthworm coelomocytes – comparative approach” (B. Swiderska, S. Kedracka-Krok, T. Panz, A.J. Morgan, A. Falniowski, P.Grzmil, B. Plytycz, 2016 [1]. Lysenin family proteins were identified based on unique peptides sequenced by tandem mass spectrometry coupled with liquid chromatography (LC-MS/MS in lumbricid earthworms Eisenia andrei and E. fetida, the latter with or without the MUG-like fluorophore. Lysenin and lysenin-related protein 2 (LRP-2, fetidin were identified in all 9 investigated specimens of Eisenia sp. LRP-1 was identified in 5 of 6 specimens of E. fetida, while LRP-3 was present in 2 of 3 investigated specimens of E. andrei. Here, the detailed characteristics of identified peptides unique to the particular members of lysenin family present in each particular earthworm specimen was provided. The information concerning mass to charge ratio, retention time, modifications and score of unique peptides was given.

Eisenia fetida increased removal of polycyclic aromatic hydrocarbons from soil

Energy Technology Data Exchange (ETDEWEB)

Silvia M. Contreras-Ramos; Dioselina Alvarez-Bernal; Luc Dendooven [Laboratory of Soil Ecology, Department of Biotechnology and Bioengineering, Mexico (Mexico)

2006-06-15

The removal of phenanthrene, anthracene and benzo(a)pyrene added at three different concentrations was investigated with or without earthworms (Eisenia fetida) within 11 weeks. Average anthracene removal by the autochthonous micro-organisms was 23%, 77% for phenanthrene and 13% for benzo(a)pyrene, while it was 51% for anthracene, 47% for benzo(a)pyrene and 100% for phenanthrene in soil with earthworms. At 50 and 100 mg phenanthrene kg{sup -1} E. fetida survival was 91% and 83%, but at 150 mg kg{sup -1} all died within 15 days. Survival of E. fetida in soil amended with anthracene {<=}1000 mg kg{sup -1} and benzo(a)pyrene {<=}150 mg kg{sup -1} was higher than 80% and without weight loss compared to the untreated soil. Only small amounts of PAHs were detected in the earthworms. It was concluded that E. fetida has the potential to remove large amounts of PAHs from soil, but more work is necessary to elucidate the mechanisms involved.

The influence of age on the composting rate of organic material by Eisenia fetida (Oligochaeta

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Editorial Office

1995-07-01

Full Text Available One of the main objectives of a vermicomposting plant is to achieve a maximum composting rate of organic waste. Apart from population densities, substrate characteristics and environmental factors, age structure of the population is expected to affect the rate of composting. The composting rate of worms was studied in the laboratory under optimal conditions over a period of 55 days. Growth and sexual maturity were monitored as well as the composting rate during various stages of the life-cycle of Eisenia fetida. The composting rate was initially slow and reached a maximum peak when the worms were pre-clitellate.

Empleo del método de secado convectivo combinado para la deshidratación de papaya (Carica papaya L.), variedad Maradol roja

OpenAIRE

Sahylin Muñiz Becerá; Antihus Hernández Gómez; Annia García Pereira; Lilia Méndez Lagunas

2013-01-01

La presente investigación tiene como objetivo evaluar el método de secado convectivo de papaya (Carica papaya L.) variedad Maradol roja, combinado con la aplicación de pretratamientos de osmosis (DOSC) y escaldado simple (ESSC), mediante el efecto de los factores tecnológi- cos del secador: temperatura (40 y 60oC) y velocidad del flujo de aire (2,5 y 1,5 m/s) sobre el comportamiento de las propiedades de calidad de la fruta deshidratada y la cinética del proceso. El pretratamiento de escaldad...

TV websites and their users: a place for transmedia storytelling. Case studies of "Águila Roja" and "Game of Thrones" in Spain

OpenAIRE

Guerrero, Mar

2014-01-01

Este artículo indaga en la complejidad de la narrativa transmedia y las producciones de los fans desde una perspectiva que combina semiótica y etnografía virtualizada. El análisis se basa en las webs oficiales en España de las series de televisión Aguila Roja (TVE) y "Juego de Tronos" (HBO). Del estudio se desprende una novedosa propuesta de roles desempeñados por los usuarios en su interacción con las webs televisivas. This paper looks into the complexity of transmedia storytelling and fa...

Different routes, same pathways: Molecular mechanisms under silver ion and nanoparticle exposures in the soil sentinel Eisenia fetida

International Nuclear Information System (INIS)

Novo, Marta; Lahive, Elma; Díez-Ortiz, María; Matzke, Marianne; Morgan, Andrew J.; Spurgeon, David J.; Svendsen, Claus; Kille, Peter

2015-01-01

Use of nanotechnology products is increasing; with silver (Ag) nanoparticles particularly widely used. A key uncertainty surrounding the risk assessment of AgNPs is whether their effects are driven through the same mechanism of action that underlies the toxic effects of Ag ions. We present the first full transcriptome study of the effects of Ag ions and NPs in an ecotoxicological model soil invertebrate, the earthworm Eisenia fetida. Gene expression analyses indicated similar mechanisms for both silver forms with toxicity being exerted through pathways related to ribosome function, sugar and protein metabolism, molecular stress, disruption of energy production and histones. The main difference seen between Ag ions and NPs was associated with potential toxicokinetic effects related to cellular internalisation and communication, with pathways related to endocytosis and cilia being significantly enriched. These results point to a common final toxicodynamic response, but initial internalisation driven by different exposure routes and toxicokinetic mechanisms. - Highlights: • Molecular effects underlying Ag ions and NPs exposure were studied in Eisenia fetida. • Full transcriptomic study of a genetically characterised lineage. • NPs and ions presented a similar toxicodynamic response. • Internalisation of the two Ag forms by different toxicokinetic mechanisms. - Transcriptomic analyses after exposure of earthworms to silver NPs or ions showed a final common toxicodynamic response, but internalisation by different toxicokinetic mechanisms

EFFICIENCY OF COMPOSTING PARTHENIUM PLANT AND NEEM LEAVES IN THE PRESENCE AND ABSENCE OF AN OLIGOCHAETE, EISENIA FETIDA

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S. Sivakumar ، H. Kasthuri ، P. Senthilkumar ، C. V. Subbhuraam ، Y. C. Song

2009-07-01

Full Text Available Parthenium plants and neem leaves were composted using the epigeic earthworm, Eisenia fetida (worm-worked compost to study the growth and reproductive indices of earthworm involved in the process of composting. Similarly, parthenium plants and neem leaves were composted without worms (worm-unworked compost. Efficacy of the resulting composts in supporting the growth of plant was tested with the germination and growth of Vigna radiate seedlings. The results showed that higher parthenium amendment significantly reduced the growth and reproduction of Eisenia fetida compared with control. The two-way ANOVA results showed a significant difference in the growth rate of worms when exposed to different amended concentrations of parthenium plants and neem leaves at different durations as fixed factors. The following compost parameters were not significantly different when compared with control: pH, nitrogen, phosphorus, iron for parthenium worm-worked compost; nitrogen, phosphorus, magnesium, iron, organic carbon and carbon/nitrogen ratio for neem worm-worked compost; nitrogen, phosphorus and organic carbon for parthenium worm-unworked compost and pH, nitrogen, phosphorus, zinc and carbon/nitrogen ratio for neem worm-unworked compost. Between parthenium plant composts and neem leaves composts, significant differences were not observed in any of the plant biometric parameters. The results obtained from the present study indicated that the parthenium composting at low amendments with cow dung may help its eradication for better utilization.

Exposure to 17β-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida.

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Zbynek Heger

Full Text Available The environmental impacts of various substances on all levels of organisms are under investigation. Among these substances, endocrine-disrupting compounds (EDCs present a threat, although the environmental significance of these compounds remains largely unknown. To shed some light on this field, we assessed the effects of 17β-oestradiol on the growth, reproduction and formation of free radicals in Eisenia fetida.Although the observed effects on growth and survival were relatively weak, a strong impact on reproduction was observed (50.70% inhibition in 100 μg/kg of E2. We further demonstrated that the exposure of the earthworm Eisenia fetida to a contaminant of emerging concern, 17β-oestradiol (E2, significantly affected the molecules involved in antioxidant defence. Exposure to E2 results in the production of reactive oxygen species (ROS and the stimulation of antioxidant systems (metallothionein and reduced oxidized glutathione ratio but not phytochelatins at both the mRNA and translated protein levels. Matrix-assisted laser desorption/ionization (MALDI-imaging revealed the subcuticular bioaccumulation of oestradiol-3,4-quinone, altering the levels of local antioxidants in a time-dependent manner.The present study illustrates that although most invertebrates do not possess oestrogen receptors, these organisms can be affected by oestrogen hormones, likely reflecting free diffusion into the cellular microenvironment with subsequent degradation to molecules that undergo redox cycling, producing ROS, thereby increasing environmental contamination that also perilously affects keystone animals, forming lower trophic levels.

In vitro anthelmintic activity and chemical composition of methanol extracts and fractions of Croton paraguayensis and Vernonia brasiliana against Eisenia fetida

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Andrea Leticia Cáceres

2017-02-01

Full Text Available Objective: To evaluate the chemical composition and the anthelmintic activity of the methanol extracts and the acid and basic fractions of Croton paraguayensis (C. paraguayensis and Vernonia brasiliana (V. brasiliana against Eisenia fetida. Methods: A preliminary phytochemical analysis was performed to assess the presence of groups of secondary metabolites. The plants were extracted with methanol to obtain the crude extracts. A differential pH extraction was performed to isolate basic compounds like alkaloids. The methanolic extracts and the fractions obtained were tested for anthelmintic activity against Eisenia fetida, using albendazole as positive control. Results: The phytochemical test demonstrated the presence of alkaloids in the crude extracts and alkaline fractions, along with flavonoids, coumarins, steroids/triterpenes and tannins. The anthelmintic activity of the extracts and fractions of C. paraguayensis and V. brasiliana showed a statistically significant decrease of the times for paralysis and death compared to albendazole. Conclusions: The methanolic extracts and fractions of C. paraguayensis and V. brasiliana contain compounds that possess anthelmintic activity. The isolation of the substances responsible for the biological effect described could result in the development of new drugs to treat helminth diseases.

Durante la administración de rojas pinilla 1953 a 1957

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William Pacheco Vargas

2007-01-01

Full Text Available Este artículo, se basa en el proyecto de investigación sobre la evolución de las finanzas de la Universidad Pedagógica de Colombia en Tunja, durante los años de gobierno del tunjano, Teniente General Gustavo Rojas Pinilla. Se analiza así mismo, los antecedentes y disposiciones presupuestales, el origen de los ingresos recaudados y el destino de los mismos y el impacto en el desarrollo institucional especialmente en las nuevas dependencias y en las ya existentes, incorporadas con motivo de su creación; medible a través de la participación en el gasto ejecutado.Lo que finalmente, permite establecer el balance financiero de la institución. A manera de conclusión, se determina el costo real promedio por estudiante para cada año en la Sección Universitaria. En los años de 1955 y 1957, se comparan las dependencias, la proporción de estudiantes, el presupuesto ejecutado, el costo por alumno, el número de docentes y empleados, y los correspondientes índices de crecimiento. De esta manera, se calcula el costo por escolar para toda la institución, sin importar el nivel de enseñanza de la misma; confrontando los datos con los cálculos hechos por ASCUN-FUN.

Self-assemblage and quorum in the earthworm Eisenia fetida (Oligochaete, Lumbricidae.

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Lara Zirbes

Full Text Available Despite their ubiquity and ecological significance in temperate ecosystems, the behavioural ecology of earthworms is not well described. This study examines the mechanisms that govern aggregation behaviour specially the tendency of individuals to leave or join groups in the compost earthworm Eisenia fetida, a species with considerable economic importance, especially in waste management applications. Through behavioural assays combined with mathematical modelling, we provide the first evidence of self-assembled social structures in earthworms and describe key mechanisms involved in cluster formation. We found that the probability of an individual joining a group increased with group size, while the probability of leaving decreased. Moreover, attraction to groups located at a distance was observed, suggesting a role for volatile cues in cluster formation. The size of earthworm clusters appears to be a key factor determining the stability of the group. These findings enhance our understanding of intra-specific interactions in earthworms and have potential implications for extraction and collection of earthworms in vermicomposting processes.

Biological effects of decabromodiphenyl ether (BDE209) and Pb on earthworm (Eisenia fetida) in a soil system

International Nuclear Information System (INIS)

Li, Jing; Zhang, Wei; Chen, Lin; Liang, Jun; Lin, Kuangfei

2015-01-01

BDE209 and Pb are ubiquitous contaminants at e-waste recycling sites (EWRSs). This study aimed to determine acute and sub-acute toxicity to earthworm Eisenia fetida induced by BDE209 and Pb in natural soil. Results demonstrated that the inhibition of Pb on growth and reproduction of earthworms followed a dose-dependent pattern. Earthworms exposed to 100 mg kg −1 of BDE209 displayed avoidance responses, while the soil indicated a more obvious decline of habitat function with the increase of Pb level. Comet assay suggested that increasing concentrations of Pb exposure resulted in a gradual increase in the tail length and olive tail moment, which meant that the degree of DNA damage was promoted. BDE209 addition could reduce the damage; therefore the joint effects of both chemicals showed antagonistic. These results revealed that joint exposure (BDE209-Pb) could elicit pronounced biochemical and physiological responses in earthworms, and the DNA damage might be potential molecular biomarker of the two pollutants. - Highlights: • 48 h and 14 d LC 50 values of BDE209 were lower than that of Pb, suggesting Pb was more toxic to earthworms. • Inhibition of Pb on the growth and reproduction of earthworms followed certain dose–response relationships. • Joint effects of BDE209 and Pb on earthworms might be antagonistic in comet assay. - This study demonstrated the biological effects of BDE209-Pb joint exposure on earthworm Eisenia fetida in a soil system for the first time.

Tolerance Test of Eisenia Fetida for Sodium Chloride

Energy Technology Data Exchange (ETDEWEB)

Kerr, M.; Stewart, A.J.

2003-01-01

Saltwater spills that make soil excessively saline often occur at petroleum exploration and production (E&P) sites and are ecologically damaging. Brine scars appear when produced water from an E&P site is spilled onto surrounding soil, causing loss of vegetation and subsequent soil erosion. Revegetating lands damaged by brine water can be difficult. The research reported here considers earthworms as a bioremedial treatment for increasing the salt mobility in this soil and encouraging plant growth and a healthy balance of soil nutrients. To determine the practical application of earthworms to remediate brine-contaminated soil, a 17-d test was conducted to establish salt tolerance levels for the common compost earthworm (Eisenia fetida) and relate those levels to soil salinity at brine-spill sites. Soil samples were amended with sodium chloride in concentrations ranging from 1 to 15 g/kg, which represent contamination levels at some spill sites. The survival rate of the earthworms was near 90% in all tested concentrations. Also, reproduction was noted in a number of the lower-concentration test replicates but absent above the 3-g/kg concentrations. Information gathered in this investigation can be used as reference in further studies of the tolerance of earthworms to salty soils, as results suggest that E. fetida is a good candidate to enhance remediation at brine-damaged sites.

Evaluation of the Toxicity, AChE Activity and DNA Damage Caused by Imidacloprid on Earthworms, Eisenia fetida.

Science.gov (United States)

Wang, Kai; Qi, Suzhen; Mu, Xiyan; Chai, Tingting; Yang, Yang; Wang, Dandan; Li, Dongzhi; Che, Wunan; Wang, Chengju

2015-10-01

Imidacloprid is a well-known pesticide and it is timely to evaluate its toxicity to earthworms (Eisenia fetida). In the present study, the effect of imidacloprid on reproduction, growth, acetylcholinesterase (AChE) and DNA damage in earthworms was assessed using an artificial soil medium. The median lethal concentration (LC50) and the median number of hatched cocoons (EC50) of imidacloprid to earthworms was 3.05 and 0.92 mg/kg respectively, the lowest observed effect concentration of imidacloprid about hatchability, growth, AChE activity and DNA damage was 0.02, 0.5, 0.1 and 0.5 mg/kg, respectively.

Pertumbuhan cacing tanah Eisenia fetida sp. Pada kompos limbah fleshing

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Prayitno Prayitno

2015-12-01

Full Text Available The use of earthworm as a source of income and a means for managing organic solid waste such as fleshing waste has been widely applied. The aim of this research was to find the optimum ratio between fleshing waste and cow dung in the growing medium of Eisenia fetida sp. This research was conducted by growing the E. fetida sp. earthworm in the medium containing fleshing waste mixed with cow dung. The ratio of fleshing waste and cow dung was varied at 0:100; 10:90; 20:80; 30:70; 40:60; and 50:50. In addition, 2 parts of chopped stubbles per part of every sample was added as a carbon source. The prepared media were fermented for three weeks with EM4 as a starter, followed by incubation of the earthworm for six weeks. The weight and the number of earthworm were evaluated every two weeks. The optimum growth of earthworm was achieved at the 2nd week of incubation with 185.48% and 121.10% increase of weight and number of earthworm, respectively, at 40:60 ratio of fleshing waste and cow dung in the growing medium.

Toxicity of selected organic chemicals to the earthworm Eisenia fetida

Energy Technology Data Exchange (ETDEWEB)

Neuhauser, E.F.; Loehr, R.C.; Malecki, M.R.; Milligan, D.L.; Durkin, P.R.

A number of methods recently have been developed to biologically evaluate the impact of man's activities on soil ecosystems. Two test methods, the 2-d contact test and the 14-d artificial soil test, were used to evaluate the impact of six major classes of organic chemicals on the earthworm Eisenia fetida (Savigny). Of the organic chemicals tested, phenols and amines were the most toxic to the worms, followed in descending order of toxicity by the substituted aromatics, halogenated aliphatics, polycyclic aromatic hydrocarbons, and phthalates. No relationship was found between earthworm toxicity as determined by the contact test and rat, Rattus norvegicus Berkenhout and mouse, Mus musculus L. LD/sub 50/ values. The physicochemical parameters of water solubility, vapor pressure, and octanol/water partition coefficient for the chemicals tested in the contact test did not show a significant relationship to the E. fetida LC/sub 50/ values. These studies indicate that: (i) earthworms can be a suitable biomonitoring tool to assist in measuring the impact of organic chemicals in wastes added to soils and (ii) contact and artificial soil tests can be useful in measuring biological impacts.

Effect of Butachlor Herbicide on Earthworm Eisenia fetidaIts Histological Perspicuity

International Nuclear Information System (INIS)

Gobi, M.; Gunasekaran, P.

2010-01-01

With the advent of the Green Revolution, there has been a quantum leap in the use of synthetic herbicides and pesticides throughout the world to sustain high yielding crop varieties. Continuous use of these synthetic chemicals leads to loss of soil fertility and soil organisms. To explore the effect of exposure to commercial herbicide (Butachlor) on the life history parameters (biomass, clitellum development, and cocoon production) and the histological changes in the earthworm Eisenia fetida over 60 days, the dried cow dung was contaminated with 0.2575 mg/ kg -1 , 0.5150 mg/ kg -1 , and 2.5750 mg/ kg -1 of butachlor based on the LC 50 value, and a control was maintained. The mean earthworm biomass was found to be decreased with increasing herbicide concentration. Similarly, cocoon production was also reduced by the increasing herbicide concentration. A possible explanation is an increased demand for energy, needed for the regulation and detoxification of herbicide. All earthworms in the exposed group were found to have glandular cell enlargement and to be vacuolated

Effect of Butachlor Herbicide on Earthworm Eisenia fetida—Its Histological Perspicuity

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Muthukaruppan Gobi

2010-01-01

Full Text Available With the advent of the Green Revolution, there has been a quantum leap in the use of synthetic herbicides and pesticides throughout the world to sustain high yielding crop varieties. Continuous use of these synthetic chemicals leads to loss of soil fertility and soil organisms. To explore the effect of exposure to commercial herbicide (Butachlor on the life history parameters (biomass, clitellum development, and cocoon production and the histological changes in the earthworm Eisenia fetida over 60 days, the dried cow dung was contaminated with 0.2575 mg kg−1, 0.5150 mg kg−1, and 2.5750 mg kg−1 of butachlor based on the LC50 value, and a control was maintained. The mean earthworm biomass was found to be decreased with increasing herbicide concentration. Similarly, cocoon production was also reduced by the increasing herbicide concentration. A possible explanation is an increased demand for energy, needed for the regulation and detoxification of herbicide. All earthworms in the exposed group were found to have glandular cell enlargement and to be vacuolated.

Selección masal por peso y coloración en tilapia roja

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María Alejandra Ruiz Peña

2009-04-01

Full Text Available Para evaluar la efectividad de la selección masal por color y peso se analizaron dos generaciones (G1 y G2 de alevinos de tilapia roja Oreochromis sp. De 40.000 larvas a la sexta semana se eliminaron los alevinos manchados y blancos y a las 14 semanas se separaron por sexos. Se midió el peso (g, la longitud total (cm, la altura (cm y el ancho (cm de 150 individuos a las 6, 14 y 24 semanas de edad. En la semana 24 se escogieron 150 machos y 450 hembras con las coloraciones deseables y mayor peso. La selección resultó efectiva para coloración en G2, puesto que la proporción de individuos rojos se incrementó en 15% con respecto a los testigos. Entre generaciones (G1 y G2 el efecto de la selección fue positivo, debido a que la proporción de rojos se incrementó de 64% a 84% y se redujo la de manchados de 31% a 13%. En ambas generaciones los machos fueron significativamente más pesados que las hembras. Se encontraron notables diferencias entre generaciones para peso y talla, puesto que los selectos superaron al control en 27% y 8% (G1 y en 22% y 11% (G2 para el peso y la talla, respectivamente.

Use of the reference organism Eisenia foetida to investigate bioaccumulation and biological effects following contamination of soil by uranium

International Nuclear Information System (INIS)

Giovanetti, A.; Cozzella, M.L.; Basso, E.; Ninova, P.; Fesenko, S.; Sansone, U.

2006-01-01

Full text of publication follows: The use of reference organisms for radiological assessments on non -human species is an integral part of the current systemic approach for the management of radiation effects in the environment. The reference organisms approach allows the evaluation of radiological impact on the environment taking into account relationships among ambient radionuclide activity concentrations, dose and expected adverse biological effects. Four broad categories of biological damages are included: mortality, morbidity, DNA damage and reproductive failure. Earthworms are one of the most important biotic components in the soil, they are commonly used in studies of toxicity and they are included in the list of the reference organisms suggested by International (ICRP) and national organisations. However, up to now, no adequate results have been obtained for earthworms allowing the identification of the dose-response relationship, essentially for the contamination scenarios where radionuclide can provide both radiation and chemical impact. Uranium (U) is a naturally occurring heavy metal. Recently there has been public concern on the presence in the environment of depleted uranium (DU), a by-product of the process used to enrich natural uranium ore for use in nuclear reactors and in nuclear weapons. The presence of uranium in soil could lead to both toxic and radiation impact and it is difficult to distinguish the different impacts and their contribution to possible biological effects. European Union, OECD and FAO have selected the earthworms Eisenia for testing soil toxicity because it is an organism that can be easily cultured in the laboratory, an extensive database is available, and it feeds at the soil surface level. The prime objective of the present study was to evaluate the possible use of Eisenia foetida as a bio-marker of U environmental impact. Four groups of six sexually mature Eisenia foetida were maintained in the dark at 21 deg. C in Petri

Reproducción de Eisenia foetida en suelos agrícolas de áreas mineras contaminadas por cobre y arsênico.

OpenAIRE

ÁVILA, G.; GAETE, H.; MORALES, M.; NEAMAN, A.

2008-01-01

El objetivo del trabajo fue evaluar la toxicidad del cobre y arsénico en suelos agrícolas, mediante bioensayos estandarizados de toxicidad aguda y crónica sobre Eisenia foetida y relacionar la respuesta de ésta con las concentraciones de cobre y arsénico en los suelos. Los suelos agrícolas fueram muestreados en las inmediaciones de áreas mineras en la cuenca del río Aconcagua, Chile. E. foetida expuesta a los suelos estudiados mostró una baja mortalidad, indicando la ausencia de toxicidad agu...

TRATAMIENTO DE EFLUENTES PISCÍCOLAS (TILAPIA ROJA EN LAGUNAS CON Azolla pinnata

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GUILLERMO CHAUX F

2013-12-01

Full Text Available Los sistemas con plantas acuáticas flotantes son una alternativa económicamente sostenible para el tratamiento de efluentes piscícolas. Se evaluó a escala piloto el desempeñ,o de un sistema de lagunas con Azolla pinnata en serie para el tratamiento de efluentes de cría de tilapia roja durante el proceso de levante y engorde. El sistema construido en la piscícola La Yunga (Popayán, Colombia consistió en dos líneas de cinco lagunas en serie; la primera con A. pinnata y la segunda sin la planta acuática; cada laguna se operó con un tiempo de detención de un día. La evaluación se realizó en época seca. La producción de Azolla fresca osciló entre 42 y 87 g/m2.d y el contenido de proteína entre 18,5% y 20,4%. Las eficiencias de remoción obtenidas en las líneas (con Azolla, sin Azolla fueron respectivamente: 56% y 46% DBO5; 49% y 26% DQO; 56% y 33% SST; 28% y 36% N-NTK; -108% y 23% N-NH4+; 64% y 34% fósforo total, mostrando superioridad del sistema con Azolla. Con solo tres lagunas en serie plantadas con A. pinnata se alcanzan las eficiencias máximas obtenidas en la remoción de DBO5, DQO, SST y fósforo total.

Micro-PIXE studies of Cd distribution in the nephridia of the earthworm Eisenia fetida (Oligochaeta)

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Prinsloo, M.W. E-mail: 9341188@narga.sun.ac.za; Reinecke, S.A.; Przybylowicz, W.J.; Mesjasz-Przybylowicz, J.; Reinecke, A.J

1999-09-02

The distribution and accumulation of Cd in the nephridia of earthworms of the species Eisenia fetida (Oligochaeta) was studied using the NAC nuclear microprobe. Worms were exposed to CdSO{sub 4} in a cattle manure substrate. Elemental maps were obtained using the true elemental imaging system (dynamic analysis). It was found that at a substrate concentration of 300 mg kg{sup -1} CdSO{sub 4}, Cd did accumulate in the nephridia, showing clear patterns in its distribution within this organ. It accumulated to the greatest extent in the region between the nephridiopore and first loop, and the urinary vasiculus, reaching values of 890 {+-} 40 mg kg{sup -1} and 570 {+-} 20 mg kg{sup -1} in these regions, respectively. This is in contrast to the lower concentrations in the body wall (76 {+-} 15 mg kg{sup -1}) of the worm.

The social forms of household production: An analysis of its construction from classification models for the Pampean case. Rojas, 2002 Las formas sociales de producción familiar: Un análisis de su construcción a partir de los modelos de clasificación para el caso pampeano. Rojas 2002

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Guillermo Oscar de Martinelli

2011-01-01

Full Text Available This article proposes an approach to social forms of household production, theoretical reflection on articulating the dimensions of interest comprising the proposed definition and identification of different subtypes farm, within the category of family farm. Also review a number of factors are considered when explaining and / or define the family nature of family production units, then move through an exercise of estimating the importance of different family subtypes. From this exercise, we will look closer to the debate on the obstacles to the development of capitalism in agriculture on the basis of the data obtained. The article is based on census data reprocessing original National Agricultural Census of 2002, on the application if the Buenos Aires de Rojas.En este artículo proponemos un abordaje de las formas sociales de producción familiar, articulando la reflexión teórica sobre las dimensiones de interés que comprenden su definición y la propuesta de identificación de distintos subtipos agrarios, dentro de la categoría de explotación familiar. Asimismo revisamos una serie de factores que suelen considerarse al momento de explicar y/o definir el carácter familiar de las unidades productivas familiares, para luego avanzar a través de un ejercicio de estimación de la importancia de los distintos subtipos familiares.A partir de este ejercicio, buscaremos acercarnos al debate sobre los obstáculos al desarrollo del capitalismo en el agro a la luz de los datos obtenidos. El artículo se basa en el reprocesamiento de los datos censales originales del Censo Agropecuario Nacional del año 2002, tomando como caso de aplicación el partido bonaerense de Rojas.

Nuevas narrativas audiovisuales: multiplataforma, crossmedia y transmedia. El caso de Águila Roja (RTVE.

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Carmen Costa Sanchez

2012-07-01

Full Text Available En la llamada era de la convergencia se ha producido la eclosión de nuevos tipos de narrativas que, basadas en las nuevas tecnologías, ponen un énfasis especial en la actividad del lector/espectador/usuario. La industria audiovisual se ha beneficiado de estas nuevas formas de contar historias, en particular, de aquellas que se valen de diferentes soportes y lenguajes para construir un relato unitario. Expresiones como multiplataforma, crossmedia o transmedia se utilizan con frecuencia para designar esta tipología de proyectos a los que se puede acceder desde diferentes plataformas con contenidos adecuados al lenguaje-forma de cada una de ellas (audiovisuales, textuales, hipertextuales, multimedia…. Entre estas narraciones, resultan de particular interés los proyectos transmedia en cuanto se trata de historias expandidas en diferentes medios y que permiten a los receptores obtener una experiencia unitaria –del contenido en un solo medio- o completa –con el disfrute de la totalidad de los componentes. En este sentido, desde la perspectiva del audiovisual español, resulta de particular interés Águila Roja. Este producto transmedia comenzó siendo una producción audiovisual convencional cuyo éxito de audiencia favoreció el desarrollo de una experiencia más amplia, que implicó el desarrollo y consolidación de su fenómeno fan.

Effect of Lignite Fly Ash on the Growth and Reproduction of Earthworm Eisenia fetida

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S. Sarojini

2009-01-01

Full Text Available Fly ash is an amorphous ferroalumino silicate, an important solid waste around thermal power plants. It creates problems leading to environmental degradation due to improper utilization or disposal. However, fly ash is a useful ameliorant that may improve the physical, chemical and biological properties of soils and is a source of readily available plant macro and micronutrients when it is used with biosolids. Supply of nutrients from fly ash with biosolids may enhance their agricultural use. The growth and reproduction of Eisenia fetida was studied during vermicomposting of fly ash with cowdung and pressmud in four different proportions (T1,T2,T3 & T4 and one control i.e., cow dung and pressmud alone. The growth, cocoon and hatchlings production were observed at the interval of 15 days over a period of 60 days. The maximum worm growth and reproduction was observed in bedding material alone. Next to that the T1 was observed as the best mixture for vermiculture.

Biodegradation of paper waste using Eisenia foetida by vermicomposting Technology

Science.gov (United States)

Mathivanan, Mahalakshmi; Aravind Vishnu Saravanan, G.; Baji, Aravindh; Manoj kumar, J.

2017-07-01

The paper wastes are being a big concern over past decades. The process of reuse of the paper wastes is employed by ‘eisenia foetida’ in Vermiculture. The paper waste in SASTRA is collected around 50kg and organic wastes like vegetable wastes and cow dung wastes are also collected. In the adjacent area of Nirman Vihar, SASTRA, the experimental setup is done in a Geosynthetic polymer bag. The area is divided into three segments and in each segment appropriate amount of paper waste and organic waste were added along with 25 numbers of earthworms. The setup is watered daily and monitored periodically and it is kindled for proper aeration. The soil samples were collected on 20 days, 45 days and 60 days from the day the earthworms were added. After 60 days of the experiment, the paper wastes, compost and earthworms are separated. The quantity of the wastes was compared to the initial amount and the composts are collected. The elemental analysis of the soil used as Vermi-bed is analyzed for improvement of soil nutrients. The vermiwashed water of the setup is analyzed for total protein. The number of earthworm is also compared to initial quantity. Out of all, the loss percentage of the organic waste and paper waste shows the degradation of the paper wastes.

Rojas Zorrilla y otros dramaturgos españoles en la biblioteca de un sefardí de Gibraltar del siglo XVIII

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Díaz-Mas, Paloma

2007-06-01

Full Text Available The manuscript Ms 22090 preserved in the National Library in Madrid is the Cancionero de Abraham Israel, a collection of poems and songs in Spanish, English and lingua franca collected by a Sephardic Jew of Gibraltar from 1761 to 1770. The manuscript also included under the title «Notta de las comedias que tengo en libros» (‘list of the plays I have in books’, a small list of 36 titles of 17th Century Spanish plays, one of whose is Rojas Zorrilla’s Progne y Filomena. The aim of this article is to identify the plays cited in that list, paying special attention to the editions along the 18th century, and to analize how Jews of Gibraltar become from early times a link between Spanish modern culture and Sephardic Jews settled in Morocco, and contributed to the re-hispanization of Morrocan Sephardic language and culture that reach its highest point in the 20th century.El manuscrito Ms 22090 de la Biblioteca Nacional de Madrid es el Cancionero de Abraham Israel, una colección de poemas y canciones en español, inglés y lingua franca recogidos por un sefardí de Gibraltar desde 1761 hasta 1770. En el manuscrito se incluye también, bajo el título «Notta de las comedias que tengo en libros» una breve lista de 36 titulos de obras teatrales españolas del siglo XVII, entre ellas Progne y Filomena, de Rojas Zorrilla. En este artículo identificamos las obras mencionadas en esa lista, con especial atención a las ediciones sueltas impresas en el siglo XVIII, y analizamos cómo los judíos de Gibraltar se convirtieron desde época temprana en un puente entre la cultura española peninsular moderna y la de los sefardíes de Marruecos, lo cual contribuyó a la rehispanización de la lengua y la cultura de los sefardíes marroquíes, que culminó en el siglo XX.

Investigations performed on the compost worm Eisenia fetida and selected species of earthworms concerning the intake of HCB and pyrene with the goal of deriving a bioaccumulation test; Untersuchungen zur Aufnahme von HCB und Pyren durch den Kompostwurm Eisenia fetida und ausgewaehlte Regenwurmwildarten. Ableitung eines Bioakkumulationstests

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Vespermann, A.; Riepert, F.; Pflugmacher, J. [Biologische Bundesanstalt fuer Land- und Forstwirtschaft, Inst. fuer Oekotoxikologie im Pflanzenschutz, Berlin (Germany)

2003-07-01

The man issues of the studies described were the validation of a test design for the assessment of the bioaccumulation potential of environmental pollutants and the applicability of Eisenia fetida as a model-organism and artificial soil (OECD) as a standard test substrate. The test organisms used were E. fetida of our own breeding stock and Allolobophora caliginosa, Allolobophora chlorotica, Allolobophora longa and Lumbricus rubellus sampled from a field site. Test soils used were the artificial soil (OECD) and a BBA field soil. Soils were each contaminated with 10 mg HCB and Pyrene per soil dry-weight. Within the test period of 4 weeks, samples were taken weekly for residue analysis in the worms and soils. Bioaccumulation factors (AF) calculated for E. fetida and the free-living species were in the range of 10-17 (HCB) and 0.9-1.7 (Pyrene) depending on the soil used. By re-calculation of the concentrations in soil to concentrations in soil water, the resulting bioconcentration factors are compared with published BCF values determined from QSAR's of other worm species and fresh water fish. It could be concluded that the existing earthworm tests (OECD 1984, ISO 1998) represent an appropriate design for testing the bioconcentration potential of chemicals in soil. (orig.) [German] Ziel der beschriebenen Untersuchungen war die praktische Ueberpruefung eines Methodenentwurfs zur Erfassung der Bioakkumulation von Umweltchemikalien mit Eisenia fetida, der Eignung von E. fetida als Modellorganismus und des OECD-Kunstbodens als Standardsubstrat. Als Testorganismen wurden aus eigener Zucht der Kompostwurm Eisenia fetida und nach Feldentnahme Allolobophora caliginosa, Allolobophora chlorotica, Allolobophora longa und Lumbricus rubellus eingesetzt. Testboeden waren der OECD-Kunstboden und ein Boden vom Versuchsfeld der BBA in Berlin-Dahlem. Beiden Boeden wurden die Testsubstanzen Hexachlorbenzol und Pyren in einer Konzentration von 10 mg/kg Bodentrockengewicht zugemischt

DYEING COTTON WITH EISENIA BICYCLIS AS NATURAL DYE USING DIFFERENT BIOMORDANTS

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BONET Mª Ángeles

2015-05-01

Full Text Available Natural dyes are known for their use in coloring of food substrate, leather as well as natural protein fibers like wool, silk and cotton as major areas of application since pre-historic times. Nowadays, there has been revival of the growing interest on the application of natural dyes on natural fibers due to worldwide environmental consciousness. Some researchers focus their studies on the improvement of these dyes using mordants. Most works use metallic mordants like aluminum or iron are used, but some of them are hazardous. In this work we used a biomordant to solve environmental problems caused by metallic mordants. The effects of chitosan weight molecular in mordanting on the dyeing characteristics and the UV protection property were examined in this study. Chitosan mordanted Eisenia Bicyclis dyed cotton showed better dyeing characteristic and higher UV protection property compared with undyed cotton fabric. To analyze the differences of the dyeing, reflection spectrophotometer was used, evaluating the results of CIELAB color difference values and the strength color (in terms of K/S value. We conclude that the type of chitosan used affect the dyeing efficiency and the UV protection, showing different behavior between dye sample using chitosan with low or medium molecular weight.

Effects of Rhamnolipid and Microbial Inoculants on the Vermicomposting of Green Waste with Eisenia fetida.

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Gong, Xiaoqiang; Wei, Le; Yu, Xin; Li, Suyan; Sun, Xiangyang; Wang, Xinyu

2017-01-01

The effects of adding the biosurfactant rhamnolipid, the lignolytic and cellulolytic fungus Phanerochete chrysosporium, and the free-living nitrogen-fixing bacterium Azotobacter chrococcum on vermicomposting of green waste with Eisenia fetida was investigated. The addition of rhamnolipid and/or either microorganism alone or in all combinations significantly increased E. fetida growth rate, the number of E. fetida juveniles and cocoons, the population densities of cellulolytic fungi and Azotobacter bacteria, and cellulase and urease activities in the vermicomposts. The quality of the final vermicompost (in terms of electrical conductivity, nutrient content, C/N ratio, humic acid content, lignin and cellulose contents, and phytotoxicity to germinating seeds) was enhanced by addition of rhamnolipid and/or microorganisms. The physical characteristics of vermicomposts produced with rhamnolipid and/or microorganisms were acceptable for agricultural application. The best quality vermicompost was obtained with the combined addition of P. chrysosporium, A. chrococcum, and rhamnolipid.

Bioaccumulation and biological effects in the earthworm Eisenia fetida exposed to natural and depleted uranium

Energy Technology Data Exchange (ETDEWEB)

Giovanetti, Anna, E-mail: anna.giovanetti@enea.i [ENEA, Institute of Radiation Protection, CR Casaccia Via Anguillarese 301, 00123 Rome (Italy); Fesenko, Sergey [International Atomic Energy Agency (IAEA), Agency' s Laboratories Seibersdorf, A-2444 Seibersdorf (Austria); Cozzella, Maria L. [ENEA, National Institute for Metrology of Ionizing Radiation, CR Casaccia Via Anguillarese 301, 00123 Rome (Italy); Asencio, Lisbet D. [Centro de Estudios Ambientales, Carretera a Castillo de Jagua, CP. 59350 C. Nuclear, Cienfuegos (Cuba); Sansone, Umberto [International Atomic Energy Agency (IAEA), Agency' s Laboratories Seibersdorf, A-2444 Seibersdorf (Austria)

2010-06-15

The accumulations of both natural (U) and depleted (DU) uranium in the earthworms (Eisenia fetida) were studied to evaluate corresponding biological effects. Concentrations of metals in the experimental soil ranged from 1.86 to 600 mg kg{sup -1}. Five biological endpoints: mortality, animals' weight increasing, lysosomal membrane stability by measuring the neutral red retention time (the NRRT), histological changes and genetic effects (Comet assay) were used to evaluate biological effects in the earthworms after 7 and 28 days of exposure. No effects have been observed in terms of mortality or weight reduction. Cytotoxic and genetic effects were identified at quite low U concentrations. For some of these endpoints, in particular for genetic effects, the dose (U concentration)-effect relationships have been found to be non-linear. The results have also shown a statistically significant higher level of impact on the earthworms exposed to natural U compared to depleted U.

Bioaccumulation and biological effects in the earthworm Eisenia fetida exposed to natural and depleted uranium

International Nuclear Information System (INIS)

Giovanetti, Anna; Fesenko, Sergey; Cozzella, Maria L.; Asencio, Lisbet D.; Sansone, Umberto

2010-01-01

The accumulations of both natural (U) and depleted (DU) uranium in the earthworms (Eisenia fetida) were studied to evaluate corresponding biological effects. Concentrations of metals in the experimental soil ranged from 1.86 to 600 mg kg -1 . Five biological endpoints: mortality, animals' weight increasing, lysosomal membrane stability by measuring the neutral red retention time (the NRRT), histological changes and genetic effects (Comet assay) were used to evaluate biological effects in the earthworms after 7 and 28 days of exposure. No effects have been observed in terms of mortality or weight reduction. Cytotoxic and genetic effects were identified at quite low U concentrations. For some of these endpoints, in particular for genetic effects, the dose (U concentration)-effect relationships have been found to be non-linear. The results have also shown a statistically significant higher level of impact on the earthworms exposed to natural U compared to depleted U.

Transcriptional responses of earthworm (Eisenia fetida) exposed to naphthenic acids in soil

International Nuclear Information System (INIS)

Wang, Jie; Cao, Xiaofeng; Sun, Jinhua; Chai, Liwei; Huang, Yi; Tang, Xiaoyan

2015-01-01

In this study, earthworms (Eisenia fetida) were exposed to commercial NAs contaminated soil, and changes in the levels of reactive oxygen species (ROS) and gene expressions of their defense system were monitored. The effects on the gene expression involved in reproduction and carcinogenesis were also evaluated. Significant increases in ROS levels was observed in NAs exposure groups, and the superoxide dismutase (SOD) and catalase (CAT) genes were both up-regulated at low and medium exposure doses, which implied NAs might exert toxicity by oxidative stress. The transcription of CRT and HSP70 coincided with oxidative stress, which implied both chaperones perform important functions in the protection against oxidative toxicity. The upregulation of TCTP gene indicated a potential adverse effect of NAs to terrestrial organisms through induction of carcinogenesis, and the downregulation of ANN gene indicated that NAs might potentially result in deleterious reproduction effects. - Highlights: • The first attempt to study gene ecotoxicity of NAs in terrestrial environment. • NAs exert toxicity by oxidative stress on earthworm. • NAs might cause carcinogenesis and reproductive disruption to earthworm. - NAs induced oxidative stress and altered transcriptions of genes involved in defense, reproduction, and carcinogenesis

Caracterización de tilapia roja (Oreochromis sp. con marcadores moleculares RAPD

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Julieta Torres Jaramillo

2010-04-01

Full Text Available Se utilizó la técnica RAPD (amplificación al azar de ADN polimórfico para el estudio de la diversidad genética de Oreochromis sp. (tilapia roja en cinco piscícolas del Valle del Cauca (Colombia y en la determinación del nivel de introgresión de las especies parentales Oreochromis mosambicus, O. niloticus y O. aureus. Se evaluaron 25 cebadores, ocho fueron polimórficos y se obtuvieron 109 bandas. Los valores de heterocigosidad esperada (0.196 a 0.256 y la estructura genética (Gst = 0.22 para Oreochromis sp. indicaron un elevado grado de polimorfismo y alta estructuración genética. Estos resultados fueron consistente con el Fst = 0.268 (P < 0.0001 dado por el Amova y el Gst = 0.040 del análisis de correspondencia múltiple. Los valores de similitud genética, el análisis de grupo, el análisis de correspondencia múltiple y el nivel de introgresion, indicaron diferencias significativas (P<0.0001 en los niveles de introgresión. El bajo nivel de diferenciación genética entre poblaciones podría ser el resultado de peces con el mismo origen genético y la alta variación dentro de poblaciones se puede presentar por prácticas de manejo. La introgresión entre piscícolas es significativa para O. aureus, mientras que las especies O. niloticus y O. mosambicus se encuentran introgresadas de forma similar en las poblaciones de Oreochromis sp.

Annotation of nerve cord transcriptome in earthworm Eisenia fetida

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Vasanthakumar Ponesakki

2017-12-01

Full Text Available In annelid worms, the nerve cord serves as a crucial organ to control the sensory and behavioral physiology. The inadequate genome resource of earthworms has prioritized the comprehensive analysis of their transcriptome dataset to monitor the genes express in the nerve cord and predict their role in the neurotransmission and sensory perception of the species. The present study focuses on identifying the potential transcripts and predicting their functional features by annotating the transcriptome dataset of nerve cord tissues prepared by Gong et al., 2010 from the earthworm Eisenia fetida. Totally 9762 transcripts were successfully annotated against the NCBI nr database using the BLASTX algorithm and among them 7680 transcripts were assigned to a total of 44,354 GO terms. The conserve domain analysis indicated the over representation of P-loop NTPase domain and calcium binding EF-hand domain. The COG functional annotation classified 5860 transcript sequences into 25 functional categories. Further, 4502 contig sequences were found to map with 124 KEGG pathways. The annotated contig dataset exhibited 22 crucial neuropeptides having considerable matches to the marine annelid Platynereis dumerilii, suggesting their possible role in neurotransmission and neuromodulation. In addition, 108 human stem cell marker homologs were identified including the crucial epigenetic regulators, transcriptional repressors and cell cycle regulators, which may contribute to the neuronal and segmental regeneration. The complete functional annotation of this nerve cord transcriptome can be further utilized to interpret genetic and molecular mechanisms associated with neuronal development, nervous system regeneration and nerve cord function.

The sublethal effects of the organochlorines dieldrin and lindane on growth and reproduction of Eudrilus eugeniae and Eisenia fetida (Oligochaeta

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Editorial Office

1994-07-01

Full Text Available Experimental exposure of the earthworm species Eudrilus eugeniae to organochlorines showed that dieldrin causes damage to sperm ultrastructure when viewed electronmicroscopically. Worms containing concentrations of 7,27 mg/kg dieldrin and higher showed more than 10% sperm damage. Exposure of Eisenia fetida to sublethal concentrations of lindane did not result in sperm damage but demonstrated an increase in growth and reproductive activity. It is argued that quantification of sperm damage and correlation with pesticide concentration could provide a useful tool for evaluating environmental quality. Furthermore, the effects of sublethal concentrations of pesticides that manifest themselves in increased growth and reproductive activity could affect ecological balances.

Biodiversity of Earthworms in Taiwan: a Species Checklist with the Confirmation and New Records of the Exotic Lumbricids Eisenia fetida and Eiseniella tetraedra

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Robert J. Blakemore

2006-09-01

Full Text Available Terrestrial megadrile earthworms variously reported from Taiwan including Lanyu Island (Botel Tobago number approximately 70 species with 29 of these, or 40%, non-natives for which 69% are Asiatic Megascolecidae and 21% are Lumbricidae of Holarctic origin. An additional 27 unnamed species have been identified from Ilan county north-eastern Taiwan by Chen et al. (2003 that, if published, would bring the total to around 100 known species. The checklist is also provided with the confirmation and new records of the exotic lumbricids Eisenia fetida (Savigny and Eiseniella tetraedra (Savigny.

The effect of the glycolipoprotein extract (G-90) from earthworm Eisenia foetida on the wound healing process in alloxan-induced diabetic rats.

Science.gov (United States)

Goodarzi, Golnaz; Qujeq, Durdi; Elmi, Maryam M; Feizi, Farideh; Fathai, Sadegh

2016-06-01

Diabetes is now regarded as a major public health problem. The number of patients is estimated to increase to over 439 million cases by 2030. One of the major health clinical problems in patients with diabetes patients is impaired wound healing. Diabetic foot ulcer is a major complication of diabetes mellitus in 12 to 25% of patients, which increases the risk of damage in the limbs or amputation. The earthworm Eisenia foetida glycolipoprotein (as known G-90) is a blend of macromolecules with some biological properties including mitogenicity, anticoagulation, fibrinolysis, bacteriostatic and antioxidatiaon. Given the biological properties of G-90, this study was conducted to investigate the effect of extract obtained from the homogenate of Eisenia foetida (G-90) on the wound healing process in alloxan-induced diabetic rats. The results of the present study revealed that treatment by using G-90 can speed up the wound healing process, which is exactly similar to the effect of D-panthenol treatment in rats. These findings also demonstrated that G-90 treatment decreases the risk of infection in the wound site compared with D-panthenol treatment. In addition, histological analysis indicated that a better extracellular matrix formation with increased fibroblast proliferation, neovascularization, collagen synthesis and early epithelial layer formation was observed in G-90 treated group. Therefore, the G-90 could be considered as a new wound healing agent introducing promising therapeutic approaches in both human and veterinary medicine. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

Role of Eisenia fetida in rapid recycling of nutrients from bio sludge of beverage industry.

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Singh, J; Kaur, A; Vig, A P; Rup, P J

2010-03-01

Beverage industry bio sludge proved to be toxic when given alone to earthworms even after stabilization for 15 days, therefore, it was mixed in various proportions with cattle dung. Best suitable mixture for highest survival, maximum growth and highest population buildup of Eisenia fetida was determined by observing mortality, growth rate, rate of cocoon production, population buildup and time taken for decomposition of waste. Minimum mortality and maximum population buildup were observed in 50:50 mixture of bio sludge and cattle dung. Nitrogen, phosphorous, sodium and pH increased in all the feed mixtures, while electrical conductivity, organic carbon and potassium declined in all the samples in comparison to traditional compost (without worms). Degradation of 50:50 mixture could be achieved in 75 days when worms were inoculated at 25 g/kg feed mixture. But the best-quality product was obtained after 105-110 days with 7.5 g worms/kg feed mixture. (c) 2009 Elsevier Inc. All rights reserved.

Serotonin-induced nitric oxide production in the ventral nerve cord of the earthworm, Eisenia fetida.

Science.gov (United States)

Kitamura, Y; Naganoma, Y; Horita, H; Ogawa, H; Oka, K

2001-10-01

Effect of serotonin on nitric oxide (NO) production in the ventral nerve cord (VNC) of the earthworm Eisenia fetida was investigated by a bio-imaging and an electrochemical technique. In the bio-imaging, the spatial pattern of NO production in VNC was visualized using an NO-specific fluorescent dye, diaminofluorescein-2 diacethyl (DAF-2 DA). Application of serotonin (100 microM) increased NO production in VNC by about 65% (PVNC. In the electrochemical technique, real-time basal and serotonin-induced NO production was estimated with an NO-specific electrode. On the ventral surface of VNC, the estimated basal NO production was stable at 200+/-52 nM, and was transiently augmented to 840+/-193 nM by the addition of 10 microM serotonin. In conclusion, the estimated basal NO production in the earthworm VNC is relatively high compared with other nervous systems earlier reported, and transiently augmented by serotonin. Our results suggest that NO signaling in VNC is involved in neuromodulation by serotonin.

Heavy Metals Bioaccumulation by Iranian and Australian Earthworms (Eisenia fetida in the Sewage Sludge Vermicomposting

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MR Shahmansouri, H Pourmoghadas, AR Parvaresh, H Alidadi

2005-01-01

Full Text Available Vermicomposting of organic waste has an important part to play in an integrated waste management strategy. In this study, the possibility of heavy metals accumulation with two groups of Iranian and Australian earthworms in sewage sludge vermicompost was investigated. Eisenia fetida was the species of earthworms used in the vermicomposting process. The bioaccumulation of Cr, Cd, Pb, Cu, and Zn as heavy metals by Iranian and Australian earthworms was studied. The results indicated that heavy metals concentration decreased with increasing vermicomposting time. Comparison of the two groups of earthworms showed that the Iranian earthworms consumed higher quantities of micronutrients such as Cu and Zn comparing with the Australian earthworms, while the bioaccumulation of non-essential elements such as Cr, Cd, and Pb by the Australian group was higher. The significant decrease in heavy metal concentrations in the final vermicompost indicated the capability of both Iranian and Australian E.fetida species in accumulating heavy metals in their body tissues.

Reproducción de Eisenia foetida en suelos agrícolas de áreas mineras contaminadas por cobre y arsénico Reproduction of Eisenia foetida in agricultural soils from mining areas contaminated with copper and arsenic

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Gonzalo Ávila

2007-03-01

Full Text Available El objetivo del trabajo fue evaluar la toxicidad del cobre y arsénico en suelos agrícolas, mediante bioensayos estandarizados de toxicidad aguda y crónica sobre Eisenia foetida y relacionar la respuesta de ésta con las concentraciones de cobre y arsénico en los suelos. Los suelos agrícolas fueram muestreados en las inmediaciones de áreas mineras en la cuenca del río Aconcagua, Chile. E. foetida expuesta a los suelos estudiados mostró una baja mortalidad, indicando la ausencia de toxicidad aguda. Además, se observó una disminución en la producción de capullos y desarrollo de juveniles, indicando la existencia de toxicidad crónica. Dicha disminución fue relacionada con las concentraciones de cobre y arsénico en los suelos. El cobre y arsénico en conjunto disminuyeron la producción de capullos, mientras que sólo el arsénico disminuyó el desarrollo de juveniles. Suelos agrícolas provenientes de áreas mineras de la cuenca poseen potenciales características tóxicas para el desarrollo de la macrofauna edáfica.The objective of this work was to evaluate copper and arsenic toxicity in agricultural soils, by means of standardized bioassays of chronic and acute toxicity on Eisenia foetida, and to relate E. foetida response to copper and arsenic concentrations in soils. The agricultural soils were sampled in the proximity of copper mining industries, in the Aconcagua river basin, Chile. E. foetida exposed to the studied soils exhibited a low mortality, indicating the absence of acute toxicity. Besides, a decrease in the cocoon production and juvenile development was observed, indicating the existence of chronic toxicity. Reduction in the cocoon production and juvenile development was related to soil concentrations of copper and arsenic. Both copper and arsenic reduced the cocoon production, while only arsenic diminished the juvenile development. Agricultural soils from mining areas of the basin have potentially toxic characteristics

Genotoxicity assessment of cobalt chloride in Eisenia hortensis earthworms coelomocytes by comet assay and micronucleus test.

Science.gov (United States)

Ciğerci, İbrahim Hakkı; Ali, Muhammad Muddassir; Kaygısız, Şöhret Yüksek; Liman, Recep

2016-02-01

Cobalt and its different compounds are extensively used worldwide and considered as possible environmental pollutant. Earthworms are useful model organism and its different species are used to monitor soil pollution. No study has been found to detect cobalt chloride (CoCl2) genotoxicity in earthworms. So, current study aimed to evaluate CoCl2 induced genotoxicity in Eisenia hortensis earthworms coelomocytes by alkaline comet assay (CA) and micronucleus (MN) test. The earthworms (n = 10 for each group) were exposed to different series of CoCl2 concentrations (100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, 600 ppm) to find LD50. The LD50 for CoCl2 was found at 226 ppm. Then, doses of LD50/2, LD50 and 2XLD50 for 48 h were used. CA and MN demonstrated the significant increase (P earthworms. Copyright © 2015 Elsevier Ltd. All rights reserved.

Caracterización de tilapia roja (Oreochromis sp. con marcadores moleculares RAPD

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Muñoz Jaime Eduardo

2010-06-01

Full Text Available Se utilizó la técnica RAPD (amplificación al azar de ADN polimórfico para el estudio de la diversidad genética de Oreochromis sp. (tilapia roja en cinco piscícolas del Valle del Cauca (Colombia y en la determinación del nivel de introgresión de las especies parentales Oreochromis mosambicus, O. niloticus y O. aureus. Se evaluaron 25 cebadores, ocho fueron polimórficos y se obtuvieron 109 bandas. Los valores de heterocigosidad esperada (0.196 a 0.256 y la estructura genética (G_st = 0.22 para Oreochromis sp. indicaron un elevado grado de polimorfismo y alta estructuración genética. Estos resultados fueron consistente con el F_st = 0.268 (P < 0.0001 dado por el Amova y el G_st = 0.040 del análisis de correspondencia múltiple. Los valores de similitud genética, el análisis de grupo, el análisis de correspondencia múltiple y el nivel de introgresion, indicaron diferencias significativas (P<0.0001 en los niveles de introgresión. El bajo nivel de diferenciación genética entre poblaciones podría ser el resultado de peces con el mismo origen genético y la alta variación dentro de poblaciones se puede presentar por prácticas de manejo. La introgresión entre piscícolas es significativa para O. aureus, mientras que las especies O. niloticus y O. mosambicus se encuentran introgresadas de forma similar en las poblaciones de Oreochromis sp.

PIGMENTACIÓN, CRECIMIENTO Y SUPERVIVENCIA DE CRÍAS CARPA KOI (CYPRINUS CARPIO VAR. HAEMATOPTERUS ALIMENTADAS CON DIETAS CON INCLUSIONES DE ACEITE DE LANGOSTILLA ROJA (PLEURONCODES PLANIPES

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Gloria L. Gil R.

2015-01-01

Full Text Available En este estudio se evaluó el efecto de la inclusión de aceite de langostilla roja (Pleuroncodes planipes sobre el crecimiento, supervivencia y pigmentación del tegumento de crías de carpa koi (Cyprinus carpio var. haematopterus. El estudio consistió en tres experimentos: experimento 1, con fotoperiodo de 4 h L / 20 h O y aguas claras; experimento 2, con fotoperiodo 12 h L/ 12 h O y aguas claras, y, experimento 3, cuyo fotoperiodo fue de 12 h L /12 O y aguas con algas. En estos experimentos, dos variedades de crías de carpa koi -naranjas y con manchas oscuras- fueron alimentadas con dietas adicionadas con aceite de langostilla a diferentes porcentajes de inclusión (0, 1, 2 y 3 %, con el 5% de alimento con relación a la biomasa de cada unidad experimental. Al final de cada uno de los experimentos se tomaron fotografías de los organismos y se analizó la coloración con el modelo de matiz-saturación-brillantez de cuatro regiones anatómicas en ambas variedades de crías y en los experimentos 1 y 2 se evaluaron parámetros biológicos: supervivencia, ganancia en longitud y en peso, y porcentaje de incremento en peso y en longitud. Las dietas adicionadas con aceite de langostilla roja no fueron rechazadas por los organismos, y no se observaron efectos negativos en las variables evaluadas. En general, la inclusión de aceite influyó en la pigmentación de las crías de color naranja y en el crecimiento y la coloración de los organismos con manchas oscuras, particularmente cuando el fotoperiodo fue de 12 L/ 12 O.

DNA damage and oxidative stress induced by imidacloprid exposure in the earthworm Eisenia fetida.

Science.gov (United States)

Wang, Juan; Wang, Jinhua; Wang, Guangchi; Zhu, Lusheng; Wang, Jun

2016-02-01

To investigate the soil ecological effect of imidacloprid, earthworm Eisenia fetida was exposed to various concentrations of imidacloprid (0.10, 0.50, and 1.00 mg kg(-1) soil) respectively after 7, 14, 21, and 28 d. The effect of imidacloprid on reactive oxygen species (ROS) generation, antioxidant enzymes activity [superoxide dismutase (SOD) and catalase (CAT), glutathione S-transferase enzyme (GST)], malondialdehyde (MDA) content and DNA damage of the E. fetida was investigated. Significant increase of the ROS level was observed. The SOD and GST activity were significantly induced at most exposure intervals. CAT activity was inhibited and reflected a dose-dependent relationship on days 7, 14 and 21. High MDA levels were observed and the olive tail moment (OTM) as well as the percentage of DNA in the comet tail (tail DNA%) in comet assay declined with increasing concentrations and exposure time after 7 d. Our results suggested that the sub-chronic exposure of imidacloprid caused DNA damage and lipid peroxidation (LPO) leading to antioxidant responses in earthworm E. fetida. Copyright © 2015 Elsevier Ltd. All rights reserved.

Uptake kinetics of metals by the earthworm Eisenia fetida exposed to field-contaminated soils

Energy Technology Data Exchange (ETDEWEB)

Nahmani, Johanne, E-mail: nahmani@univ-metz.f [Laboratoire Interactions Ecotoxicite, Biodiversite, Ecosystemes, CNRS UMR 7146, Universite Paul Verlaine - Metz, Rue du General Delestraint, 57070 Metz (France); Department of Soil Science, School of Human and Environmental Sciences, University of Reading, Whiteknights, Reading, Berkshire RG6 6DW (United Kingdom); Hodson, Mark E. [Department of Soil Science, School of Human and Environmental Sciences, University of Reading, Whiteknights, Reading, Berkshire RG6 6DW (United Kingdom); Devin, Simon [Laboratoire Interactions Ecotoxicite, Biodiversite, Ecosystemes, CNRS UMR 7146, Universite Paul Verlaine - Metz, Rue du General Delestraint, 57070 Metz (France); Vijver, Martina G. [Leiden University, Institute of Environmental Sciences (CML), P.O. Box 9518, 2300 RA Leiden (Netherlands)

2009-10-15

It is well known that earthworms can accumulate metals. However, most accumulation studies focus on Cd-, Cu-, Pb- or Zn-amended soils, additionally few studies consider accumulation kinetics. Here we model the accumulation kinetics of 18 elements by Eisenia fetida, exposed to 8 metal-contaminated and 2 uncontaminated soils. Tissue metal concentration was determined after 3, 7, 14, 21, 28 and 42 days. Metal elimination rate was important in determining time to reach steady-state tissue metal concentration. Uptake flux to elimination rate ratios showed less variation and lower values for essential than for non-essential metals. In theory kinetic rate constants are dependent only on species and metal. Therefore it should be possible to predict steady-state tissue metal concentrations on the basis of very few measurements using the rate constants. However, our experiments show that it is difficult to extrapolate the accumulation kinetic constants derived using one soil to another. - Earthworm metal uptake and elimination constants derived from a one-compartment model show little systematic variation with soil properties.

Comparative toxicity of tetra ethyl lead and lead oxide to earthworms, Eisenia fetida (Savigny)

International Nuclear Information System (INIS)

Venkateswara Rao, J.; Kavitha, P.; Padmanabha Rao, A.

2003-01-01

Leaded gasoline contains tetra ethyl lead (TEL) as an antiknocking agent, which produces major amounts of lead oxide in automobile exhaust along with traces of TEL. To minimize the lead contamination, methyl tertiary butyl ether (MTBE) is used as a substitute for producing unleaded gasoline. It has become increasingly apparent that young children are highly susceptible to the harmful effects of lead. Hence, a study was carried out to monitor lead toxicity in soil, using adult earthworms (Eisenia fetida, Savigny). Leaded gasoline (TEL) and lead oxide are 383- and 211-fold more toxic than unleaded gasoline (MTBE) in 7 days of exposure and 627- and 290-fold more toxic in 14 days, respectively. Results indicate that the presence of TEL in leaded gasoline and lead oxide has a significant effect on behavior, morphology, and histopathology of earthworms. Absorption of TEL into the tissues is comparatively less than that of lead oxide but toxic effects were severe. Rupture of the cuticle, extrusion of coelomic fluid and inflexible metameric segmentation were observed, causing desensitization of the posterior region leading to fragmentation in earthworms

Toxicity of azodrin on the morphology and acetylcholinesterase activity of the earthworm Eisenia foetida

International Nuclear Information System (INIS)

Rao, J.V.; Kavitha, P.

2004-01-01

The acute toxicity of azodrin (monocrotophos, an organophosphorus insecticide) was determined on a soil organism, Eisenia foetida. The median lethal concentrations (LC 50 ) were derived from a 48-h paper contact test and from artificial soil tests. The LC 50 of azodrin in the paper contact test was 0.46±0.1 μg cm -2 (23±6 mg L -1 ) and those in the 7- and 14-day artificial soil tests were 171±21 and 132±20 mg kg -1 , respectively. The neurotoxic potentiality of azodrin was assessed by using a marker enzyme, acetylcholinesterase (AChE; EC 3.1.1.7) in both in vitro and in vivo experiments. The progressive signs of morphological destruction are correlated with percentage inhibition of AChE in the in vivo experiments. The kinetics of AChE activity in the presence and absence of azodrin indicated that the toxicant is competitive in nature. This study demonstrated that azodrin causes concentration-dependent changes in the morphology and AChE activity of the earthworm E. foetida

Antioxidant gene expression and metabolic responses of earthworms (Eisenia fetida) after exposure to various concentrations of hexabromocyclododecane.

Science.gov (United States)

Shi, Yajuan; Xu, Xiangbo; Chen, Juan; Liang, Ruoyu; Zheng, Xiaoqi; Shi, Yajing; Wang, Yurong

2018-01-01

Hexabromocyclododecane (HBCD), a ubiquitous suspected contaminant, is one of the world's most prominent brominated flame retardants (BFRs). In the present study, earthworms (Eisenia fetida) were exposed to HBCD. The expression of selected antioxidant enzyme genes was measured, and the metabolic responses were assessed using nuclear magnetic resonance (NMR) to identify the molecular mechanism of the antioxidant stress reaction and the metabolic reactions of earthworms to HBCD. A significant up-regulation (p  0.05). Principal component analysis (PCA) of the metabolic responses showed that all groups could be clearly differentiated, and the highest concentration dose group was the most distant from the control group. Except for fumarate, the measured metabolites, which included adenosine triphosphate (ATP), valine, lysine, glycine, betaine and lactate, revealed significant (p earthworm exposure studies. Copyright © 2017 Elsevier Ltd. All rights reserved.

Amelioration of acidic soil increases the toxicity of the weak base carbendazim to the earthworm Eisenia fetida.

Science.gov (United States)

Liu, Kailin; Wang, Shaoyun; Luo, Kun; Liu, Xiangying; Yu, Yunlong

2013-12-01

Ameliorating acidic soils is a common practice and may affect the bioavailability of an ionizable organic pollutant to organisms. The toxicity of the weak base carbendazim to the earthworm (Eisenia fetida) was studied in an acidic soil (pH-H₂O, 4.6) and in the ameliorated soil (pH-H₂O, 7.5). The results indicated that the median lethal concentration of carbendazim for E. fetida decreased from 21.8 mg/kg in acidic soil to 7.35 mg/kg in the ameliorated soil. To understand why the amelioration increased carbendazim toxicity to the earthworm, the authors measured the carbendazim concentrations in the soil porewater. The authors found increased carbendazim concentrations in porewater, resulting in increased toxicity of carbendazim to earthworms. The increased pore concentrations result from decreased adsorption because of the effects of pH and calcium ions. © 2013 SETAC.

Solid waste management of temple floral offerings by vermicomposting using Eisenia fetida

International Nuclear Information System (INIS)

Singh, Akanksha; Jain, Akansha; Sarma, Birinchi K.; Abhilash, P.C.; Singh, Harikesh B.

2013-01-01

Highlights: ► Effective management of temple floral offerings using E. fetida. ► Physico-chemical properties in TW VC were better especially EC, C/N, C/P and TK. ► TW VC as plant growth promoter at much lower application rates than KW and FYW VC. - Abstract: Recycling of temple waste (TW) mainly comprising of floral offerings was done through vermitechnology using Eisenia fetida and its impact on seed germination and plant growth parameters was studied by comparing with kitchen waste (KW) and farmyard waste (FYW) vermicompost (VC). The worm biomass was found to be maximum in TW VC compared to KW and FYW VCs at both 40 and 120 days old VCs. Physico-chemical analysis of worm-worked substrates showed better results in TW VC especially in terms of electrical conductivity, C/N, C/P and TK. 10% TW VC–water extract (VCE) showed stimulatory effect on germination percentage of chickpea seeds while KW and FYW VCE proved effective at higher concentration. Variation in growth parameters was also observed with change in the VC–soil ratio and TW VC showed enhanced shoot length, root length, number of secondary roots and total biomass at 12.5% VC compared to KW and FYW VC

Solid waste management of temple floral offerings by vermicomposting using Eisenia fetida

Energy Technology Data Exchange (ETDEWEB)

Singh, Akanksha, E-mail: bhuaks29@gmail.com [Department of Botany, Banaras Hindu University, Varanasi 221 005 (India); Jain, Akansha, E-mail: akansha007@rediffmail.com [Department of Botany, Banaras Hindu University, Varanasi 221 005 (India); Sarma, Birinchi K., E-mail: birinchi_ks@yahoo.com [Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005 (India); Abhilash, P.C., E-mail: pca.iesd@bhu.ac.in [Institute for Environment and Sustainable Development, Banaras Hindu University, Varanasi 221 005 (India); Singh, Harikesh B., E-mail: hbs1@rediffmail.com [Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005 (India)

2013-05-15

Highlights: ► Effective management of temple floral offerings using E. fetida. ► Physico-chemical properties in TW VC were better especially EC, C/N, C/P and TK. ► TW VC as plant growth promoter at much lower application rates than KW and FYW VC. - Abstract: Recycling of temple waste (TW) mainly comprising of floral offerings was done through vermitechnology using Eisenia fetida and its impact on seed germination and plant growth parameters was studied by comparing with kitchen waste (KW) and farmyard waste (FYW) vermicompost (VC). The worm biomass was found to be maximum in TW VC compared to KW and FYW VCs at both 40 and 120 days old VCs. Physico-chemical analysis of worm-worked substrates showed better results in TW VC especially in terms of electrical conductivity, C/N, C/P and TK. 10% TW VC–water extract (VCE) showed stimulatory effect on germination percentage of chickpea seeds while KW and FYW VCE proved effective at higher concentration. Variation in growth parameters was also observed with change in the VC–soil ratio and TW VC showed enhanced shoot length, root length, number of secondary roots and total biomass at 12.5% VC compared to KW and FYW VC.

Die chemiese en aminosuursamestelling van die komposwurm, Eisenia fetida (Oligochaeta as potensiële proteïenbron vir dierevoeding

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A. J. Reinecke

1987-03-01

Full Text Available Daar bestaan ’n toenemende behoefte aan proteïenryke grondstowwe vir die veevoerbedryf in Suid-Afrika. Die intensiewe veebedryf en abattoirs produseer jaarliks groot hoeveelhede organiese afvalmateriaal waarvan die proteïeninhoud nie benut word nie. Die komposwurm, Eisenia fetida, kan op groot skaal op die afval geteel word. Dit is dus potensieel moontlik om dierlike afvalproteïene te herwin indien E. fetida as proteïenbron in gebalanseerde veerantsoene aangewend kan word. Daarom is die proteïenwaarde van die erdwurmspesie ondersoek deur die algemene chemiese samestelling en aminosuursamestelling te bepaal. Erdwurmmeel bevat al tien essensiële aminosure vir pluimvee, in besonder gunstige verhoudings. Daarbenewens is die listen- en metionienbeskikbaarheid van erdwurmmeel hoër as die van vismeel.

Mortality of the earthworms, Eisenia foetida, after gamma-irradiation at different stages of their life history

International Nuclear Information System (INIS)

Suzuki, Jun; Egami, Nobuo

1983-01-01

Effects of ionizing radiation on the earthworm, Eisenia foetida, were investigated by means of mortality, growth inhibition and hatchability of the eggs. Adult earthworms were resistant to 137 Cs γ-irradiation, LD 50 (30 days) value being about 65 kR. Young earthworms were less resistant than adult ones. Their growth was completely inhibited by 10 kR of γ-irradiation, but 2 kR did not affect them at all. Embryos at early developmental stages showed high mortality by γ-irradiation and as development proceeded their resistance to irradiation increased. Mature earthworms were irradiated and the hatchability of the cocoons laid by them after irradiation was examined. Hatchability of the cocoons laid within 5 days after irradiation with 2 kR was scarcely affected. Then the hatchability decreased with the interval between irradiation and laying, and reached lowest level 20 days after irradiation. From 20 days after irradiation, it started to recover and reached the control level 30 days after irradiation. (author)

Cruz Vermelha Brasileira (filial São Paulo na imprensa (1916-1930 Cruz Roja Brasileña (filial São Paulo en la imprenta (1916-1930 Brazilian Red Cross (São Paulo branch in media (1916-1930

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Fernando Porto

2009-09-01

Full Text Available Este estudo teve como objeto dimensionar a visibilidade da Cruz Vermelha Brasileira, Filial de São Paulo, na imprensa escrita no período de 1916-1930. Seus objetivos foram: descrever e analisar sua visibilidade na imprensa escrita e discutir os efeitos da crença simbólica da Cruz Vermelha Brasileira à sociedade. Os documentos de análise foram oriundos de um portfólio, do Centro Histórico Cultural da Enfermagem Ibero-americana, da Escola de Enfermagem, da Universidade de São de Paulo. Os resultados foram analisados com base nas concepções de Pierre Bourdieu, que apontaram para o círculo da crença simbólica, quando foram veiculadas 1.089 notícias na imprensa nacional e internacional. Neste sentido, a cada publicação de notícia sobre a Cruz Vermelha Brasileira, esta divulgava o órgão central no Brasil e, consequentemente, também a Cruz Vermelha Internacional.Este estudio tuvo como objeto dimensionar la visibilidad de la Cruz Roja Brasileña, filial de São Paulo, en la imprenta escrita, en el período de 1916-1930. Sus objetivos fueron: describir y analizar su visibilidad en la imprenta escrita y discutir los efectos de la creencia simbólica de la Cruz Roja Brasileña de la Sociedad. Los documentos de análisis fueron derivados de un portafolio del Centro Histórico Cultural de la Enfermería Ibero-americana, de la Escuela de Enfermería de la Universidad de São de Paulo. Los resultados fueron analizados con base en las concepciones de Pierre Bourdieu que apuntaron para el círculo de la creencia simbólica, cuando fueron publicadas 1.089 noticias en la imprenta nacional e internacional. En este sentido, a cada publicación de una noticia de la Cruz Roja Brasileña, esta divulgaba el órgano central en Brasil y consecuentemente la Cruz Roja Internacional.The objective of the present study is to dimension the Brazilian Red Cross, Branch of the State of São Paulo's visibility in the written media during the period of 1916

Cinética de secado de fruta bomba (Carica papaya L., cv. Maradol Roja) mediante los métodos de deshidratación osmótica y por flujo de aire caliente

OpenAIRE

Daybelis Fernández Valdés; Sahylin Muñiz Becerá; Annia García Pereira; Rafael Cervantes Beyra; Dayvis Fernández Valdés

2015-01-01

La deshidratación es uno de los métodos más utilizados para la conservación de frutas y vegetales, mediante su uso se logra extender los períodos de almacenamiento preservando siempre la calidad de los productos. El presente trabajo tiene como objetivo evaluar el comportamiento cinético de las principales propiedades de la fruta bomba (Carica papaya L, cv. Maradol Roja) durante los procesos agroindustriales de deshidratación osmótica (DO) y por flujo de aire caliente (DAC). Las frutas se cort...

Response to increasing levels of NK in the production of onion (Allium cepa L. var. “Roja Arequipeña”

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Julio Estuardo Amaya Robles

2013-03-01

Full Text Available With the objective to determine the influence of increasing levels of nitrogen and potassium in the growth and yield of onion bulbs, an experiment was carried in the Experimental Institute for Agricultural Research and Technology located in Pampas de San Juan, Laredo, La Libertad (Peru. The experimental design was randomized complete block with 3x4 factorial arrangement with three replicates and twelve treatments resulting from combinations 60, 120, 180 and 240 kg N ha-1 , and 40, 80 and 160 kg K2O has -1 , using 80 kg as a single dose of P2O5.ha-1 . We used certified seed onion var. "Roja Arequipeña”. Linear responses were obtained for the weight of bulbs with yields of 1.010 and 1.006 g -1 average of 10 bulbs, with combinations of N120xK80 x K80 and N60, and quadratic response to N dose of 120 kg.ha-1 with which promoted the increased production of onion bulbs.

Selección masal por peso y coloración en tilapia roja

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Ruíz Peña María Alejandra

2009-06-01

Full Text Available Para evaluar la efectividad de la selección masal por color y peso se analizaron dos generaciones (G₁ y G₂ de alevinos de tilapia roja Oreochromis sp. De 40.000 larvas a la sexta semana se eliminaron los alevinos manchados y blancos y a las 14 semanas se separaron por sexos. Se midió el peso (g, la longitud total (cm, la altura (cm y el ancho (cm de 150 individuos a las 6, 14 y 24 semanas de edad. En la semana 24 se escogieron 150 machos y 450 hembras con las coloraciones deseables y mayor peso. La selección resultó efectiva para coloración en G₂, puesto que la proporción de individuos rojos se incrementó en 15% con respecto a los testigos. Entre generaciones (G₁ y G₂ el efecto de la selección fue positivo, debido a que la proporción de rojos se incrementó de 64% a 84% y se redujo la de manchados de 31% a 13%. En ambas generaciones los machos fueron significativamente más pesados que las hembras. Se encontraron notables diferencias entre generaciones para peso y talla, puesto que los selectos superaron al control en 27% y 8% (G₁ y en 22% y 11% (G₂ para el peso y la talla, respectivamente.

Manual de transferencia tecnológica: Proceso de elaboración de salchichas a partir de tilapia roja (Oreochromis sp) con adición de almidón de sagú (Marantha arundinacea)

OpenAIRE

Hleap Zapata, José Igor; Molina Cortés, Andrea

2008-01-01

La industrialización de los productos agropecuarios es una alternativa para buscar la solución mercantil en las comunidades rurales del país, por lo que se busca generar una unidad de negocio por medio del procesado de la tilapia roja y con esto fomentar este hábito en las comunidades agrarias a los jóvenes productores. En el interior de este documento usted encontrará un extracto del proyecto investigativo realizado en el trabajo de grado de la estudiante de Ingeniería Agroindustrial Andrea ...

Evidence for Bioavailability of Au Nanoparticles from Soil and Biodistribution within Earthworms (Eisenia fetida)

Energy Technology Data Exchange (ETDEWEB)

J Unrine; S Hunyadi; O Tsyusko; W Rao; A Shoults-Wilson; P Bertsch

2011-12-31

Because Au nanoparticles (NPs) are resistant to oxidative dissolution and are easily detected, they have been used as stable probes for the behavior of nanomaterials within biological systems. Previous studies provide somewhat limited evidence for bioavailability of Au NPs in food webs, because the spatial distribution within tissues and the speciation of Au was not determined. In this study, we provide multiple lines of evidence, including orthogonal microspectroscopic techniques, as well as evidence from biological responses, that Au NPs are bioavailable from soil to a model detritivore (Eisenia fetida). We also present limited evidence that Au NPs may cause adverse effects on earthworm reproduction. This is perhaps the first study to demonstrate that Au NPs can be taken up by detritivores from soil and distributed among tissues. We found that primary particle size (20 or 55 nm) did not consistently influence accumulated concentrations on a mass concentration basis; however, on a particle number basis the 20 nm particles were more bioavailable. Differences in bioavailability between the treatments may have been explained by aggregation behavior in pore water. The results suggest that nanoparticles present in soil from activities such as biosolids application have the potential to enter terrestrial food webs.

Cinética hematopoyética en Tilapia roja ( Oreochromis sp : Modelo basado en el efecto genotóxico con radiación ionizante

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M. Peñaloza

2001-07-01

Full Text Available Para elaborar un programa de biomonitoreo en peces se requiere conocer la cinética hema-topoyética en estos organismos y detectar el daño en la sangre circulante. La frecuencia deformación de micronúcleos espontánea e inducida en un tiempo determinado se necesita paraconfirmar la utilidad de estas especies para ensayos de genotoxicidad. En los peces, el órganohematopoyético es la porción cefálica del riñón. Mediante el efecto clastogénico que produce laradiación ionizante a bajas dosis se puede determinar la cinética generativa de eritrocitos.El objetivo de esta investigación es relacionar la formación de micronucleos inducida porradiación ionizante con la cinética hematopoyética en tilapia roja.

Lead and zinc bioavailability to Eisenia fetida after phosphorus amendment to repository soils

International Nuclear Information System (INIS)

Ownby, David R.; Galvan, Kari A.; Lydy, Michael J.

2005-01-01

Four phosphorus forms were investigated as potential soil amendments to decrease the bioavailability of Pb and Zn in two repository soils to the earthworm, Eisenia fetida. Treatments were evaluated by examining differences in bioaccumulation factors between amended and non-amended soils. Triple super phosphate at 5000 mg P/kg decreased both Pb and Zn bioavailability in both soils. Rock phosphate at 5000 mg P/kg decreased Zn bioavailability, but not Pb bioavailability in both repository soils. Monocalcium phosphate and tricalcium phosphate at 5000 mg P/kg did not significantly decrease Pb or Zn bioavailability to earthworms in either repository soil. In order to optimize phosphorus amendments, additional phosphorus (up to 15,000 mg P/kg) and lowered pH were used in a series of tests. The combination of lowering the pH below 6.0 and increasing phosphorus concentrations caused complete mortality in all triple super phosphate amended soils and partial mortality in the highest rock phosphate amended soils. Results indicate that triple super phosphate and rock phosphate are viable soil amendments, but care should be taken when optimizing amendment quantity and pH so that adverse environmental effects are not a by-product. - Phosphorus form and pH were controlling factors in the effectiveness of phosphorus amendment in decreasing Pb and Zn bioavailability

Subacute toxicity of copper and glyphosate and their interaction to earthworm (Eisenia fetida)

International Nuclear Information System (INIS)

Zhou, Chui-Fan; Wang, Yu-Jun; Li, Cheng-Cheng; Sun, Rui-Juan; Yu, Yuan-Chun; Zhou, Dong-Mei

2013-01-01

Glyphosate (GPS) and copper (Cu) are common pollutants in soils, and commonly co-exist. Due to the chemical structure of GPS, it can form complexes of heavy metals and interface their bioavailability in soil environment. In order to explore the interactions between GPS and Cu, subacute toxicity tests of Cu and GPS on soil invertebrate earthworms (Eisenia fetida) were conducted. The relative weight loss and whole-worm metal burdens increased significantly with the increasing exposure concentration of Cu, while the toxicity of GPS was insignificant. The joint toxicity data showed that the relative weight loss and the uptake of Cu, as well as the superoxide dismutase, catalase and malondialdehyde activities, were significantly alleviated in the present of GPS, which indicated that GPS could reduce the toxicity and bioavailability of Cu in the soil because of its strong chelating effects. Highlights: •Cu markedly increased the weight loss ratio of earthworm. •Cu decreased the cocoon production of earthworm. •The toxicity of GPS on earthworm was insignificant. •The presence of GPS could reduce the toxicity of Cu on earthworm. -- The presence of glyphosate could reduce the toxicity and bioavailability of Cu in the soil because of its strong chelating effects

Lead and zinc bioavailability to Eisenia fetida after phosphorus amendment to repository soils

Energy Technology Data Exchange (ETDEWEB)

Ownby, David R. [Fisheries and Illinois Aquaculture Center and Department of Zoology, Southern Illinois University, Carbondale, IL 62901 (United States); Galvan, Kari A. [Fisheries and Illinois Aquaculture Center and Department of Zoology, Southern Illinois University, Carbondale, IL 62901 (United States); Lydy, Michael J. [Fisheries and Illinois Aquaculture Center and Department of Zoology, Southern Illinois University, Carbondale, IL 62901 (United States)]. E-mail: mlydy@siu.edu

2005-07-15

Four phosphorus forms were investigated as potential soil amendments to decrease the bioavailability of Pb and Zn in two repository soils to the earthworm, Eisenia fetida. Treatments were evaluated by examining differences in bioaccumulation factors between amended and non-amended soils. Triple super phosphate at 5000 mg P/kg decreased both Pb and Zn bioavailability in both soils. Rock phosphate at 5000 mg P/kg decreased Zn bioavailability, but not Pb bioavailability in both repository soils. Monocalcium phosphate and tricalcium phosphate at 5000 mg P/kg did not significantly decrease Pb or Zn bioavailability to earthworms in either repository soil. In order to optimize phosphorus amendments, additional phosphorus (up to 15,000 mg P/kg) and lowered pH were used in a series of tests. The combination of lowering the pH below 6.0 and increasing phosphorus concentrations caused complete mortality in all triple super phosphate amended soils and partial mortality in the highest rock phosphate amended soils. Results indicate that triple super phosphate and rock phosphate are viable soil amendments, but care should be taken when optimizing amendment quantity and pH so that adverse environmental effects are not a by-product. - Phosphorus form and pH were controlling factors in the effectiveness of phosphorus amendment in decreasing Pb and Zn bioavailability.

DNA adduct quantification in Eisenia fetida after subchronic exposures to creosote contaminated soils

Energy Technology Data Exchange (ETDEWEB)

Charrois, J.W.A.; McGill, W.B. [Alberta Univ., Dept. of Renewable Resources, Edmonton, AB (Canada)

1999-07-01

Within soil ecosystems contaminant toxicity can vary from acute and chronic, depending on the time of exposure. Due to the long times involved chronic toxicity is difficult to determine. DNA adducts fall into the category of biochemical markers that act as an early warning system in environmental monitoring. It has been proposed that they could be used as a sensitive method to determine environmental exposures to compounds such as polycyclic aromatic hydrocarbons (PAHs), which can occur, although not exclusively, in creosote. In this connection, Benzo[a]pyrene (BaP) is a PAH that can be transformed into an electrophilic metabolite, which ultimately results in DNA adduct formation. Use was made of a 32P postlabeling method to quantify the number of DNA adducts occurring in the earthworm Eisenia fetida after exposure to weathered creosote contaminated- and biotreated-soils with and without additions of extra BaP. DNA adducts can be measured in earthworms exposed to creosote contaminated- and biotreated-soils. E. fetida exposed to weathered creosote contaminated soils had significantly more DNA adducts than those exposed to a pristine control soil. Exposures to creosote contaminated soils with additional BaP (1000 mg/kg) or biotreatment did not yield statistically significant increases in DNA adducts compared to the pristine control. (Abstract only)

Cocomposting with and without Eisenia fetida for conversion of toxic paper mill sludge to a soil conditioner.

Science.gov (United States)

Kaur, Arvinder; Singh, Jaswinder; Vig, Adarsh Pal; Dhaliwal, S S; Rup, Pushpinder J

2010-11-01

Present study was envisaged for fast bioremediation of toxic paper mill sludge into a soil ameliorating agent. Although a rich source of organic carbon this sludge cannot be directly applied in fields and is recycled very slowly in landfills as it is deficient in other nutrients. Therefore it was mixed with cattle dung in various proportions and subjected to aerobic cocomposting (without worms) and vermicocomposting (with Eisenia fetida). It was observed that mixing cattle dung with the sludge improved physico-chemical characteristics (with transition metals in the permissible range for manures) of the products of both the processes and enhanced its acceptability for worms. Higher decline in organic carbon and higher content of nitrogen and phosphorous along with lower electrical conductivity and higher pH of the products of vermicomposting indicated that E. fetida helped in fast conversion of toxic paper mill sludge into a soil conditioner in 100days. Copyright 2010 Elsevier Ltd. All rights reserved.

Bioconversion of garden waste, kitchen waste and cow dung into value-added products using earthworm Eisenia fetida.

Science.gov (United States)

Wani, K A; Mamta; Rao, R J

2013-04-01

Solid waste management is a worldwide problem and it is becoming more and more complicated day by day due to rise in population, industrialization and changes in our life style. Transformation of industrial sludges into vermicompost is of double interest: on the one hand, a waste is converted into value added product, and, on the other, it controls a pollutant that is a consequence of increasing industrialization. Garden waste, kitchen waste and cow dung were subjected to recycle through vermicomposting by using the epigeic earthworm Eisenia fetida under field conditions. The pH, moisture content, total organic carbon, humus, nitrogen, phosphorous and potassium in vermicompost was analysed. It was found that moisture content, total organic carbon, humus, nitrogen, phosphorous and potassium was high in cow dung, followed by kitchen waste and garden waste. This study clearly indicates that vermicomposting of garden waste, kitchen waste and cow dung can not only produce a value added produce (vermicomposting) but at the same time reduce the quantity of waste.

Vulcanismo submarino del Santoniense en el Subbético: datación con nannofósiles e interpretación (formación Capas Rojas, Alamedilla, provincia de Granada

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de Gea, G.

1998-12-01

Full Text Available Submarine volcanic rocks (pillow lavas appear into the marly limestones and marls of the Capas Rojas Formation in the Median Subbetic. The dating with nannoplankton of the immediately underlying and overlying materials to these volcanic rocks has allow us to precise the age of this volcanic activity as Late Santonian (nannofossil biozone of Rucinolithus hayi. This volcanic event arose 84 million years ago, according to the absolute age obtained with the calibration of this biozone. It is the last Mesozoic volcanic activity registered until now in the External Zones of the Betic Cordilleras. The age of this event corresponds to the ending part of the interval of the Sudiberian continental margin evolution as an extensive margin, relatively nearly to the time in which it evolved to a convergent marginRocas volcánicas submarinas (lavas almohadilladas aparecen intercaladas entre calizas margosas y margas de la Formación Capas Rojas del Subbético Medio. La datación con nannoplancton de los materiales inmediatamente infrayacentes y suprayacentes a estas rocas volcánicas ha permitido precisar la edad de este vulcanismo como Santoniense superior (biozona de nannofósiles de Rucinolithus hayi. Este evento volcánico ocurrió hace unos 84 millones de años de acuerdo con la calibración a tiempos absolutos de la biozona indicada. Se trata por tanto de la actividad volcánica mesozoica más moderna datada hasta el momento en el conjunto de las Zonas Externas de las Cordilleras Béticas. La edad de este evento corresponde a la parte terminal del intervalo de tiempo durante el cual el margen continental sudibérico constituyó un margen extensivo, relativamente cercano al momento en que ocurrió su transformación a un margen convergente.

The hormetic effect of cadmium on the activity of antioxidant enzymes in the earthworm Eisenia fetida

International Nuclear Information System (INIS)

Zhang Yan; Shen Guoqing; Yu Yueshu; Zhu Hongling

2009-01-01

The hormetic dose-response relationships induced by environmental toxic agents are often characterized by low-dose stimulation and high-dose inhibition. Confirmation of the general phenomenon of hormesis may have significant implications for ecological risk assessment, although the mechanisms that underlie hormesis remain an enigma. In this study, a model-based approach for describing a dose-response relationship incorporating the hormetic effect was applied to the detection and estimation of the hormetic effect of cadmium (Cd) on the activity of antioxidant enzymes in the earthworm Eisenia fetida. The results showed that Cd at low concentrations induced an increase in the activity of catalase and superoxide dismutase (SOD), but high concentrations inhibited the enzymes, and this was reflected in an inverted U-shaped curve. The maximum hormetic magnitude of SOD activity was higher than that of catalase. The presence of hormesis induced by cadmium in the earthworm may be related to activation of adaptive pathways. - A model-based approach and careful preliminary experiments are needed for detecting and estimating the hormetic effect.

BIOCONCENTRATION OF HEAVY METALS IN VERMICOMPOSTING EARTHWORMS (Eisenia fetida, Perionyx excavatus and Lampito mauritii IN NEPAL

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Raju Panday

2014-04-01

Full Text Available Vermicomposting of organic waste can play an important part during the waste management process in larger cities such as Kathmandu where 70% of the waste generated is organic. In this study, the possibility of heavy metal (Pb, Cd, Cu and Cr bioaccumulation by three different species of earthworms Eisenia fetida, Lampito mauritii and Perionyx excavatus in domestic waste vermicompost was investigated. Quantification of heavy metals by Atomic Absorption Spectroscopy(AAS in final vermicompost showed a significant reduction in concentration of metals, Pb (11.4-26.0%, Cd (48-61%, Cu (4.9- 29.01% and Cr (18.90-33.60% at the end. Bioaccumulation of heavy metal in the composting earthworms was also recorded. Comparison of the three groups of earthworms showed that the bioaccumulation of Pb, Cu and Cr was greater for P. excavatus whereas E. fetida was the most reluctant. Heavy metal content in the vermicompost was within the limit of USEPA for Biosolids and the compost could be used for the agriculture purpose.

An enzyme from the earthworm Eisenia fetida is not only a protease but also a deoxyribonuclease.

Science.gov (United States)

Pan, Rong; Zhou, Yuan; He, Hai-Jin; He, Rong-Qiao

2011-04-01

The earthworm enzyme Eisenia fetida Protease-III-1 (EfP-III-1) is known as a trypsin-like protease which is localized in the alimentary canal of the earthworm. Here, we show that EfP-III-1 also acts as a novel deoxyribonuclease. Unlike most DNases, this earthworm enzyme recognizes 5'-phosphate dsDNA (5'P DNA) and degrades it without sequence specificity, but does not recognize 5'OH DNA. As is the case for most DNases, Mg(2+) was observed to markedly enhance the DNase activity of EfP-III-1. Whether the earthworm enzyme functioned as a DNase or as a protease depended on the pH values of the enzyme solution. The protein acted as a protease under alkaline conditions whereas it exhibited DNase activity under acid conditions. At pH 7.0, the enzyme could work as either a DNase or a protease. Given the complex living environment of the earthworm, this dual function of EfP-III-1 may play an important role in the alimentary digestion of the earthworm. Copyright © 2011 Elsevier Inc. All rights reserved.

The hormetic effect of cadmium on the activity of antioxidant enzymes in the earthworm Eisenia fetida

Energy Technology Data Exchange (ETDEWEB)

Zhang Yan [Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240 (China); Shen Guoqing, E-mail: gqsh@sjtu.edu.c [Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240 (China); Yu Yueshu; Zhu Hongling [Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240 (China)

2009-11-15

The hormetic dose-response relationships induced by environmental toxic agents are often characterized by low-dose stimulation and high-dose inhibition. Confirmation of the general phenomenon of hormesis may have significant implications for ecological risk assessment, although the mechanisms that underlie hormesis remain an enigma. In this study, a model-based approach for describing a dose-response relationship incorporating the hormetic effect was applied to the detection and estimation of the hormetic effect of cadmium (Cd) on the activity of antioxidant enzymes in the earthworm Eisenia fetida. The results showed that Cd at low concentrations induced an increase in the activity of catalase and superoxide dismutase (SOD), but high concentrations inhibited the enzymes, and this was reflected in an inverted U-shaped curve. The maximum hormetic magnitude of SOD activity was higher than that of catalase. The presence of hormesis induced by cadmium in the earthworm may be related to activation of adaptive pathways. - A model-based approach and careful preliminary experiments are needed for detecting and estimating the hormetic effect.

COMPORTAMIENTO DEL CRECIMIENTO EN ALTURA DE Hibiscus elatus Sw CULTIVADA EN CONTENEDORES

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M. Cobas-López

2003-01-01

Full Text Available Hibiscus elatus Sw. (majagua es una especie nativa de Cuba, incluida en los planes de reforestación del país por su importancia económica, ecológica y su condición de ser una madera preciosa. En el trabajo se describe el comportamiento en vivero del crecimiento en altura de la especie, cultivada en contenedores. Se utilizaron cuatro tipos de sustratos, que fueron los siguientes: S1- Turba (40 %, humus de lombriz (40 % y corteza de pino compostada (20 %; S2- Estiércol de caballo (45 %, composta (40 %, y humus de lombriz (15 %; S3- Humus de lombriz (30 %, composta (25 %, turba (25 %, y estiércol de caballo (20 %; S4- Testigo, consistente en suelo proveniente de una plantación de la especie. A través del análisis de regresión se probaron nueve modelos matemáticos del tipo y = f (x, polinómicos, exponenciales y logarítmicos. De acuerdo con el coeficiente de determinación obtenido, del análisis de residuos y la validación de los modelos, se comprobó que la función que mejor representa el crecimiento en altura de Hibiscus elatus Sw., para los sustratos estudiados fue b t y b e 1 = 0 + .

A comparison of POPs bioaccumulation in Eisenia fetida in natural and artificial soils and the effects of aging.

Science.gov (United States)

Vlčková, Klára; Hofman, Jakub

2012-01-01

The close relationship between soil organic matter and the bioavailability of POPs in soils suggests the possibility of using it for the extrapolation between different soils. The aim of this study was to prove that TOC content is not a single factor affecting the bioavailability of POPs and that TOC based extrapolation might be incorrect, especially when comparing natural and artificial soils. Three natural soils with increasing TOC and three artificial soils with TOC comparable to these natural soils were spiked with phenanthrene, pyrene, lindane, p,p'-DDT, and PCB 153 and studied after 0, 14, 28, and 56 days. At each sampling point, total soil concentration and bioaccumulation in earthworms Eisenia fetida were measured. The results showed different behavior and bioavailability of POPs in natural and artificial soils and apparent effects of aging on these differences. Hence, direct TOC based extrapolation between various soils seems to be limited. Copyright © 2011 Elsevier Ltd. All rights reserved.

Utilização de casca de arroz e esterco bovino como substrato Eisenia fetida Savigny (1826 Rice husk and cattle manure used as substrate for the Eisenia fetida Savigny (1826 multiplication

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Zaida Inês Antoniolli

2009-06-01

Full Text Available A utilização de minhocas, principalmente para a alimentação animal, vem sendo adotada e estudada, pois esses organismos apresentam em sua constituição vitaminas e aminoácidos. Entre os materiais possíveis de serem utilizados na minhocultura, atualmente incentiva-se o aproveitamento de resíduos agroindustriais de alta disponibilidade, como a casca de arroz no Rio Grande do Sul. Objetivou-se, neste trabalho, avaliar a eficiência de substratos à base de casca de arroz e esterco bovino na multiplicação e produção de biomassa de minhocas (Eisenia fetida. A população de minhocas foi avaliada quanto ao número de cocons, ao índice de multiplicação (população final/população inicial e à biomassa de minhocas frescas e secas. A avaliação foi realizada aos 60 dias após a instalação do experimento, com quatro repetições. A análise estatística dos resultados mostrou que a inclusão de casca de arroz favoreceu o desenvolvimento e a taxa de reprodução das minhocas. A maior biomassa de minhocas foi obtida nos tratamentos: 50% casca de arroz inteira e 50% esterco bovino, esterco bovino mais casca de arroz inteira tratada com hidróxido de amônio a 20 e 40%, e esterco bovino mais casca de arroz moída tratada com hidróxido de sódio a 10 e 40%. Conclui-se que a adição de casca de arroz ao esterco bovino favorece a multiplicação e o desenvolvimento de E. fetida.The use of earthworms for animal feeding has been adopted and studied, because these organisms are rich in vitamins and essential amino acids. Among the organic materials that could be used in the earthworm breeding, highly available agroindustrial residues such as rice husk are being currently used in Rio Grande do Sul. The aim of this work was to evaluate substrate based on rice husk and cattle manure on the multiplication and biomass of earthworms (Eisenia fetida. In this work, the population of earthworms, the cocoon numbers, the multiplication index, as well as

Acute toxicity of chemical pesticides and plant-derived essential oil on the behavior and development of earthworms, Eudrilus eugeniae (Kinberg) and Eisenia fetida (Savigny).

Science.gov (United States)

Vasantha-Srinivasan, Prabhakaran; Senthil-Nathan, Sengottayan; Ponsankar, Athirstam; Thanigaivel, Annamalai; Chellappandian, Muthiah; Edwin, Edward-Sam; Selin-Rani, Selvaraj; Kalaivani, Kandaswamy; Hunter, Wayne B; Duraipandiyan, Veeramuthu; Al-Dhabi, Naif Abdullah

2018-04-01

Comparative toxicity of two chemical pesticides (temephos and monocrotophos) versus a plant-derived betel leaf oil Piper betle (L.) to earthworm Eudrilus eugeniae (Kinberg) and redworm Eisenia fetida Savigny, historically: Eisenia foetida (Savigny 1826), was evaluated. Mortality rate was more prominent in temephos at 100 μg concentration to both the earthworms in filter paper test (FPT) as well as 10 mg concentration in artificial soil test (AST). In contrast, P. betle does not display much mortality rate to both the earthworms even at 1000 mg of treatment concentrations. The lethal concentration (LC 50 ) value was observed at 3.89 and 5.26 mg/kg for temephos and monocrotophos against E. eugeniae and 3.81 and 5.25 mg/kg to E. fetida, respectively. Whereas, LC 50 value of betel leaf oil was only observed at 3149 and 4081 mg/kg to E. eugeniae and E. fetida, respectively. Correspondingly, the avoidance or attraction assay also displayed that earthworms were more sensitive to the soil containing chemical pesticides. Whereas, the avoidance percentage was decreased in the P. betle oil. Similarly, sublethal concentration of chemical pesticides (5 and 6.5 mg) significantly reduced the earthworm weight and growth rate. However, P. betle oil did not change the developmental rate in the duration of the assay (2, 7 and 14 days) even at 4000 mg treatment concentration. The enzyme ratio of CAT and SOD was also affected significantly after exposure to the chemical pesticides (6.5 mg/kg). Hence, our study implied the risk assessment associated with the chemical pesticides and also recommends plant-derived harmless P. betle oil against beneficial species as an alternative pest control agent.

Bioconversion of garden waste, kitchen waste and cow dung into value-added products using earthworm Eisenia fetida

Science.gov (United States)

Wani, K.A.; Mamta; Rao, R.J.

2013-01-01

Solid waste management is a worldwide problem and it is becoming more and more complicated day by day due to rise in population, industrialization and changes in our life style. Transformation of industrial sludges into vermicompost is of double interest: on the one hand, a waste is converted into value added product, and, on the other, it controls a pollutant that is a consequence of increasing industrialization. Garden waste, kitchen waste and cow dung were subjected to recycle through vermicomposting by using the epigeic earthworm Eisenia fetida under field conditions. The pH, moisture content, total organic carbon, humus, nitrogen, phosphorous and potassium in vermicompost was analysed. It was found that moisture content, total organic carbon, humus, nitrogen, phosphorous and potassium was high in cow dung, followed by kitchen waste and garden waste. This study clearly indicates that vermicomposting of garden waste, kitchen waste and cow dung can not only produce a value added produce (vermicomposting) but at the same time reduce the quantity of waste. PMID:23961230

TRAVESTIS Y VECINOS DE LA 'ZONA ROJA' DE PALERMO: DISTANCIAS Y CERCANÍAS EN CONFLICTO. CIUDAD DE BUENOS AIRES, 1998-2012

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Martín Boy

Full Text Available Resumen En este artículo se analizará la 'zona roja' del barrio porteño de Palermo, como un conflicto urbano que devino un problema político. Vecinos, travestis, funcionarios públicos, autoridades religiosas y empresarios se manifestaron acerca de la oferta de sexo de travestis en las calles del barrio. Las diferentes argumentaciones pusieron en debate cuál era el proyecto de ciudad que debía promoverse desde las políticas públicas y quién merecía vivir en la ciudad y quién no. En este trabajo se analiza lo que sucede cuando la desigualdad social se encuentra en el espacio público. Los repertorios morales de los diferentes actores involucrados pusieron en tensión cuán tolerable podía ser la presencia de prácticas e identidades ilegítimas y cómo, desde la gestión pública, la administración de las distancias y las cercanías espaciales entre los diferentes se convirtió en un elemento vital para solucionar un conflicto urbano y moral.

Avoidance test with Eisenia fetida as indicator for the habitat function of soils. Results of a laboratory comparison test

Energy Technology Data Exchange (ETDEWEB)

Hund-Rinke, K. [Fraunhofer Inst. for Molecular Biology and Applied Ecology, Schmallenberg (Germany); Achazi, R.; Warnecke, D. [Free Univ. of Berlin, Inst. for Biology, Berlin (Germany); Roembke, J. [ECT Oekotoxikologie GmbH, Floersheim (Germany)

2003-07-01

Intention, Goal, Scope, Background. The habitat function of soils is often assessed using the reproduction test with Eisenia fetida. As this test is rather labour-intensive, an alternative is needed which is less cost-intensive in terms of duration and workload, but gives reasonable results. The avoidance test with E. fetida is a suitable screening test meeting these criteria. However, before a novel test system can be generally recommended it has to be ensured that comparable results are acquired from different laboratories on the basis of the respective test guideline. Objective. The avoidance test with E. fetida was performed as laboratory comparison test. The results were compared with those of the earthworm acute and reproduction tests carried out with the same soils. Methods. The three tests were performed by three laboratories using eight contaminated soils and three control soils. The contaminated soils were mixed with the control soils to obtain different concentrations of the contamination. (orig.)

The Earthworm Eisenia fetida Can Help Desalinate a Coastal Saline Soil in Tianjin, North China.

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Tao Zhang

Full Text Available A laboratory microcosm experiment was conducted to determine whether the earthworm Eisenia fetida could survive in a saline soil from a field site in North China, and an experiment using response surface methodology was conducted at that field site to quantify the effects of E. fetida and green waste compost (GWC on the salt content of the soil. The microcosm results showed that E. fetida survived in GWC-amended saline soil and increased the contents of humic acid, available N, and available P in the GWC-amended soil. The data from the field experiment were described by the following second-order model: [Formula in text], where y is the decrease in soil salinity (g of salt per kg of dry soil relative to the untreated control, x1 is the number of E. fetida added per m2, and x2 is the quantity of GWC added in kg per m2. The model predicted that the total salt content of the saline soil would decrease by > 2 g kg(-1 (p<0.05 when 29-90 individuals m-2 of E. fetida and 6.1-15.0 kg m(-2 of GWC were applied. We conclude that the use of E. fetida for soil desalination is promising and warrants additional investigation.

Estrategias de afrontamiento: un programa de entrenamiento para paramédicos de la Cruz Roja

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María José Pérez Molina

2011-12-01

Full Text Available La presente investigación se basa en el diagnóstico sobre las estrategias de afrontamiento utilizadas por un grupo de once técnicos en emergencias médicas (TEM de la Cruz Roja Costarricense. Se hizo un análisis integrativo por medio de la triangulación de datos obtenidos en entrevistas y observaciones realizadas durante y posterior a las emergencias tomando como categorías de estudio las situaciones de emergencia y las estrategias de afrontamiento. Los datos se codificaron y categorizaron mediante el programa de análisis cualitativo Atlas.ti 5.0. Desde el enfoque teórico de la crisis, los hallazgos del diagnóstico mostraron afectadas todas las áreas conductuales, afectivas, somáticas, interpersonales y cognoscitivas, así como el área espiritual. Los factores desencadenantes del estrés en los TEM remitieron más a aspectos contextuales que rodearon a las situaciones atendidas, que a las mismas situaciones de emergencia. Las estrategias de afrontamiento, a pesar de ser funcionales, no resultaron ser las más saludables. Con base en los resultados, se elaboró y aplicó un programa de intervención psicológico para la promoción y puesta en práctica de nuevas estrategias de afrontamiento y una adecuada translaboración de pensamientos, emociones y conductas. La investigación muestra la necesidad de potenciar la salud de manera integral de la población a través de las áreas de autocuidado, habilidades sociales, expresión de sentimientos y manejo del estrés, además hace necesario superar, a nivel institucional, la falta de comunicación y conocimiento entre los TEM y la Unidad de Soporte Psicológico.

Estudio de los polisacáridos del alga roja Polysiphonia nigrescens, cationización de agarosa, formación de complejos interpolielectrolito. Aplicaciones en floculación de coloides y liberación controlada de drogas

OpenAIRE

Prado, Héctor Juan

2010-01-01

En este Trabajo de Tesis se estudiaron los polisacáridos del alga roja Polysiphonia nigrescens, recolectada en las costas de Cabo Corrientes (Mar del Plata). Este alga biosintetiza agaranos sulfatados parcialmente ciclados y altamente sustituidos en C-6, principalmente con sulfato, aunque también se han encontrado éteres metílicos y ramificaciones simples de β-D-xilosa. Las dos díadas presentes en los galactanos de esta especie son: G6S→L6S (precursora) y G6S→LA. También se aisló una fracción...

Bioaccumulation studies with Eisenia fetida using an established degradation test system

Energy Technology Data Exchange (ETDEWEB)

Norr, C.; Riepert, F. [Biological Research Centre for Agriculture and Forestry, Inst. for Ecotoxicology and Ecochemistry in Plant Protection, Berlin (Germany)

2007-12-15

Background. Earthworms are considered as an appropriate test system to assess the bioaccumulation potential of substances in the terrestrial environment. For regulatory purposes test methods were developed and incorporated in the validation process. A test design that is particularly suited for testing {sup 14}C-labelled substances will be described here. This design was adapted from an established degradation test system for bioaccumulation tests with earthworms in soil. The antibiotic sulfadiazine was used as test substance in this study. Due to the biological activity and the widespread entering into soil by manure application, veterinary medicines have become the target of ecotoxicological risk assessment. A German research group provided the soil samples mixed with liquid pig manure, which contained the {sup 14}C-labelled test substance after having passed through the gut of animals. This exposure pathway reflects the real environmental conditions. Therefore, sulfadiazine was tested even though a significant bioaccumulation potential was not expected to be detected owing to its chemical properties. Methods. Two adult earthworms of the species Eisenia fetida were inserted in a 500 ml glass container filled with 100 g soil. The test substrate consisted of soil mixed with liquid manure containing {sup 14}C-sulfadiazine. The glass containers were fitted with a soda-lime trap, which is permeable for oxygen but absorbs produced CO{sub 2} and other volatile metabolites. As food source for the earthworms, 5 g of mashed potato powder was applied per test container at the beginning of the test. The levels of radioactivity in soil samples and earthworms were determined by combustion in an oxidizer over a four-week exposure phase. The test design follows the instructions described by the OECD draft guideline for testing the bioaccumulation behaviour of chemicals with oligochaetes in soil. (orig.)

Effects of decabromodiphenyl ether (BDE-209) on the avoidance response, survival, growth and reproduction of earthworms (Eisenia fetida).

Science.gov (United States)

Xie, Xianchuan; Qian, Yan; Wu, Yingxin; Yin, Jun; Zhai, Jianping

2013-04-01

The effects of decabromodiphenyl ether (BDE-209) on avoidance response, survival, growth, and reproduction of earthworms (Eisenia fetida) were investigated under laboratory conditions using natural and artificial soils as substrate. Results showed that no significant avoidance response was observed when earthworms were exposed to 0.1-1000 mg/kg of BDE-209 for 48 h. After 28-days exposure, no significant effects on survival and growth of adult earthworms was induced by 0.1-1000 mg/kg of BDE-209 indicating the Lowest Observed Effect Level (LOEL) of BDE-209 on their survival and body weight was more than 1000 mg/kg. Except for a significant decrease in the number of juveniles per hatched cocoon in artificial soils at 1000 mg/kg of BDE-209, no significant effects on reproductive parameters (e.g. cocoon production per earthworms, weight per cocoon and cocoon hatchability) were observed. These results suggest that adult earthworms have a strong tolerance for BDE-209 exposure in soils, but a potential toxicity does exist for earthworm embryos or juveniles. Copyright © 2012 Elsevier Inc. All rights reserved.

Nutrient Status of Vermicompost of Urban Green Waste Processed by Three Earthworm Species Eisenia fetida, Eudrilus eugeniae, and Perionyx excavatus

International Nuclear Information System (INIS)

Pattnaik, S.; Vikram, M.

2010-01-01

Major nutrient status of vermicompost of vegetable market waste (MW) and floral waste (FW) processed by three species of earthworms namely, Eudrilus eugeniae, Eisenia fetida, and Perionyx excavatus and its simple compost were assessed across different periods in relation to their respective initiative substrates. Their physical parameters temperature, moisture, ph, and electrical conductivity were also recorded. The nutrients nitrogen, phosphorus, potassium, calcium, and magnesium increased in the vermicompost and compost while the organic carbon, C/N and C/P ratios decreased as the composting process progressed from 0 to 15, 30, 45, and 60 days. The nutrient statuses of vermicomposts of all earthworm species produced from both the wastes were more than that of the compost and that of their respective substrates. Moreover, the vermicompost produced by E. eugeniae possessed higher nutrient contents than that of E. fetida, P. excavatus, and compost. The MW showed higher nutrient contents than the FW. Thus, vermicomposting is the paramount approach of nutrient recovery of urban green waste.

TRATAMIENTO DE SUSTRATO DE BOVINO Y PRODUCCIÓN DE BIOGÁS EN UN BIODIGESTOR CONTINUO CON LOMBRICULTURA.

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Hugo J. Coss y León M.

2015-01-01

Full Text Available El cuidado del medio ambiente toma mayor importancia cada día y la producción de biogás por medio de biodigestores continuos cuenta como una bioremediación al generar tierra fértil para el campo, sin embargo el proceso de producción de biogás y de esa tierra es largo, por ello, esta investigación lleva como objetivo principal el estudiar el Tratamiento de Sustrato de Bovino y Producción de Biogás en un Biodigestor Continúo con Lombricultura. El presente estudio se realizó en la planta de lombricultura del Centro Universitario de Ciencias Biológicas y Agropecuarias de la Universidad de Guadalajara, con un procedimiento metodológico de medición del pH con el fin de obtener un alimento de forma anaerobia apto para la lombriz, así como la producción de biogás para observar el comportamiento de las bacterias en conjunto al conteo de microorganismos de bacterias, hongos y actinomicetos. Se mantuvieron las mismas condiciones climáticas en dos biodigestores tipo salchicha de 5 metros de largo a temperatura ambiente y humedad interna de 75%, base húmeda para garantizar las mismas condiciones. Con 100 kg de estiércol bovino y mezclados con 300L de agua cada uno, se preparó una población de 1 kg de lombriz en un metro cuadrado de tierra como población inicial. En los resultados obtenidos se observó que el estiércol precomposteado en el biodigestor fue apto para que la lombriz se alimentara, puesto que se detectó crecimiento de la población de lombriz, igualmente se encontró producción de biogás en el contenedor de control, utilizando el hydrogen sulfide meter- modelo z-900 xp en dicho tanque, además se detectó la generación de bacterias metanogénicas y termófilas por el aumento de temperatura y diferencias en el tiempo de incubación en la siembra de bacterias, de igual forma se exhibe un suelo fértil al ser rico en hongos, bacterias y actinomicetos.

EFECTO DE FECHA Y TIPO DE PODA EN FRAMBUESA ROJA 'Malling autumn Bliss'

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R. Á. Parra-Quezada

2007-01-01

Full Text Available Se estudiaron fechas de poda al ras del suelo y despuntes en frambuesa roja 'Malling autumn Bliss', en Guerrero, Chihuahua, con el objetivo de conocer los efectos en el crecimiento de los tallos nuevos, periodo de cosecha, rendimiento y calidad de fruta. Se utilizaron plantas de un año de edad y durante el 2001 se realizaron podas al ras del suelo el 15 de marzo, 15 de abril y 15 de mayo, así como poda de despunte el 15 de marzo del mismo año y el testigo sin despuntar y sin poda. En el 2002 se evaluaron podas al ras el 15 de marzo, 15 de abril y el 15 de mayo. En ambos años se utilizaron cuatro repeticiones y unidades experimentales de dos metros de longitud. El distanciamiento entre hileras fue de 1.5 m y se formaron setos de 60 cm de ancho. Antes de la plantación (abril del 2000, el terreno se barbechó y se incorporaron con rastra entre los 10 y 15 cm de profundidad 60 ton·ha-1 de estiércol bovino seco como única fuente nutrimental al cultivo. El sistema de riego fue por goteo superficial, con goteros cada metro. La poda al ras del 15 de abril presentó el mayor rendimiento con 7.4 y 5.0 t·ha-1 en el 2001 y 2002, respectivamente. La poda al ras el 15 de mayo retrasa la fecha de floración y la cosecha en ambos años. La floración de frambuesa evade las heladas tardías. La producción se concentró de agosto a octubre. El número de nuevos tallos por metro lineal fue superior en la poda al ras del 15 de abril. Los frutos de mayor tamaño se produjeron en la poda al ras del 15 de marzo y el 15 de abril y va de 1.8 a 3.6 g por fruto. Se observa una tendencia de disminución de rendimiento conforme pasan los años de producción.

Growth, reproduction and biochemical toxicity of chlorantraniliprole in soil on earthworms (Eisenia fetida).

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Liu, Tong; Wang, Xiuguo; Chen, Dan; Li, Yiqiang; Wang, Fenglong

2018-04-15

Diamide insecticides have become the fourth most commonly used insecticide class in the world. Chlorantraniliprole (CAP) is a first-generation diamide insecticide with broad application potential. In this experiment, the eco-toxicity of CAP in soil at 0.1, 1.0, 5.0 and 10.0mg/kg on earthworms (Eisenia fetida) was evaluated during a 42 d exposure. More specifically, the environmental fate and transport of CAP between soil and earthworms was monitored during the exposure period. The present results indicated that the CAP contents of 0.1, 1.0, 5.0 and 10.0mg/kg treatments decreased to no more than 20% in the soil after 42 d of exposure. The accumulation of CAP in earthworms was 0.03, 0.58, 4.28 and 7.21mg/kg earthworm (FW) at 0.1, 1.0, 5.0 and 10.0mg/kg after 42 d of exposure. At 0.1mg/kg and 1.0mg/kg, CAP had no effect on earthworms during the exposure period. The weight of earthworms was significantly reduced at 5.0 and 10.0mg/kg at 28 and 42 days after CAP application. After the 14th day, CAP induced excess production of reactive oxygen species (ROS) at 5.0 and 10.0mg/kg, resulting in oxidative damage to biomacromolecules. We believe that CAP has a high risk potential for earthworms when used at 5.0 and 10.0mg/kg. Copyright © 2017 Elsevier Inc. All rights reserved.

Investigation of the toxicokinetics of petroleum hydrocarbon distillates with the earthworm Eisenia andrei.

Science.gov (United States)

Cermak, Janet; Stephenson, Gladys; Birkholz, Detlef; Dixon, D George

2013-04-01

The Canada-wide standards for petroleum hydrocarbons in soils regulate petroleum hydrocarbons based on four distillate ranges: F1 (C6-C10), F2 (>C10-C16), F3 (>C16-C34), and F4 (>C34). Previous toxicity tests with earthworms and F2, as well as two subfractions of F3, F3a (>C16-C23) and F3a (>C23-C34), indicate that test durations might not be sufficiently long to reach threshold effect concentrations, likely because of the differing toxicokinetics for each distillate. A study was conducted to determine the toxicokinetics of both aliphatic and aromatic fractions of F2, F3a, and F3b with the earthworm Eisenia andrei. Peak accumulation curves were observed for F2 aliphatics and aromatics and F3a aromatics, likely as a result of changes in exposure concentration over the test duration via loss or a decrease in the bioavailable fraction. Biota-soil accumulation factors were >1 for total F2 aliphatics and aromatics and F3a aromatics as well as for several individual polyaromatic hydrocarbons for each distillate. Aromatics were disproportionately accumulated over aliphatics and were the main contributors to toxicity; therefore, aromatics and aliphatics should be regulated separately. The toxicokinetics were used to interpret previous toxicity data. Higher molecular weight distillates need longer-than-standard test durations to determine toxicity, so toxicity test results from fixed, standard-duration tests are not strictly comparable for these petroleum distillates. Copyright © 2013 SETAC.

Impact of imidacloprid residues on the development of Eisenia fetida during vermicomposting of greenhouse plant waste.

Science.gov (United States)

Fernández-Gómez, Manuel J; Romero, Esperanza; Nogales, Rogelio

2011-09-15

Pesticide application in agriculture causes residues in post-harvest plant waste at different concentrations. Knowledge concerning how pesticide concentrations in such waste affect earthworms is essential for recycling greenhouse plant debris through vermicomposting. Here, we have evaluated the effects of imidacloprid (IMD) residues on earthworms (Eisenia fetida) during the vermicomposting of plant waste from greenhouse crops in Spain. Before, the effect of different IMD concentrations on earthworms was tested using cattle manure as an optimum waste for worm development. The results after using cattle manure indicate that IMD dose ≥ 5 mg kg(-1) hinders worm growth and even causes death, whereas IMD dose ≤ 2 mg IMD kg(-1) allows worm growth similar to control but impedes reproduction. The results from the vermicomposting of plant waste reveal that IMD inhibits adequate worm growth and increases mortality. Although 89% worms became sexually mature in substrate containing 2 mg IMD kg(-1), they did not produce cocoons. IMD also affected microorganisms harboured in the substrates for vermicomposting, as indicated by the reduction in their dehydrogenase activity. This enzyme activity was restored after vermicomposting. This study provides a sound basis for the vermicomposting of pesticide-contaminated plant waste. Copyright © 2011 Elsevier B.V. All rights reserved.

Biochemical responses and DNA damage in earthworms (Eisenia fetida) induced by ionic liquid [omim]PF6.

Science.gov (United States)

Liu, Xiaoyan; Zhang, Shumin; Wang, Jinhua; Wang, Jun; Shao, Yuting; Zhu, Lusheng

2016-04-01

Ionic liquids that are not that "green" to many organisms have recently been identified. This study examined the subchronic toxicity of the ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate ([omim]PF6) to earthworms (Eisenia fetida). Earthworms were exposed for a 28-day period (sampled on days 7, 14, 21, and 28) at concentrations of 0, 5, 10, 20, and 40 mg/kg. The levels of reactive oxygen species (ROS), antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POD)), detoxifying enzyme (glutathione S-transferase (GST)), lipid peroxidation, and DNA damage were measured. ROS significantly accumulated in all the treatment groups; the maximum ROS content was 51.9% higher than the control at 40 mg/kg [omim]PF6 on day 28. Increased SOD activities attenuated over the time of exposure, while the CAT activities of the treatment groups were similar to the controls, except on day 14. Furthermore, the activities of POD and GST were stimulated. Lipid peroxidation in earthworms was not apparent at 5 and 10 mg/kg [omim]PF6 but was quite obvious at 40 mg/kg [omim]PF6. In addition, DNA damage was dose- and time-dependent. In conclusion, [omim]PF6 caused oxidative stress and genotoxicity in earthworms.

Accumulation of {sup 14}C-trinitrotoluene and related nonextractable (bound) residues in Eisenia fetida

Energy Technology Data Exchange (ETDEWEB)

Belden, Jason B., E-mail: jbelden@okstate.edu [Department of Zoology, Oklahoma State University, Stillwater, OK 74078 (United States); Lotufo, Guillerme R. [US Army Engineer Research and Development Center, Vicksburg, MS 39180 (United States); Chambliss, C. Kevin [Department of Chemistry, Baylor University, Waco, TX 76798 (United States); Fisher, Jonathan C. [Department of Zoology, Oklahoma State University, Stillwater, OK 74078 (United States); Johnson, Dave R.; Boyd, Robert E.; Sims, Jerre G. [US Army Engineer Research and Development Center, Vicksburg, MS 39180 (United States)

2011-05-15

To determine if trinitrotoluene (TNT) forms nonextractable residues in earthworms and to measure the relative degree of accumulation as compared to TNT and its deaminated metabolites, Eisenia fetida was exposed to {sup 14}C-TNT using dermal contact to filter paper or exposure to soil. Nonextractable residues made up 32-68% of total body burden depending on exposure media and depuration time. Parent TNT accounted for less than 3% of radioactivity, while ADNTs accounted for 7-38%. Elimination half-lives were 61-120 h for TNT, ADNTs, and DANTs, which was significantly lower than the half-lives found for nonextractable residues, 201-240 h. However, over 80% of the nonextractable residue was solubilized using weak acid (pH 2). Based on our findings that TNT accumulation occurs primarily as nonextractable residues, which have a longer half-life, and that nonextractable residues can be solubilized, we propose that nonextractable residues could be used as a selective biomarker for assessing TNT contamination. - Highlights: > Trinitrotoluene accumulation in earthworms primarily occurs as nonextractable residues. > Nonextractable residues have a significantly longer half life in the worm as compared to TNT and its solvent-extractable deaminated metabolites. > Nonextractable residue may be useful as a biomarker for exposure to TNT. - The majority of trinitrotoluene accumulation in earthworms occurs as nonextractable residues that have a significantly longer half life in the worm as compared to TNT and its solvent-extractable deaminated metabolites.

Ternary toxicological interactions of insecticides, herbicides, and a heavy metal on the earthworm Eisenia fetida

International Nuclear Information System (INIS)

Wang, Yanhua; Chen, Chen; Qian, Yongzhong; Zhao, Xueping; Wang, Qiang

2015-01-01

Highlights: • The combined toxicity of insecticides, herbicides, and a heavy metal was examined. • Acute earthworm toxicity assays were conducted in twenty-one ternary mixtures. • Synergism predominated in the majority of the mixtures at low effect levels. • Combination index method could more accurately predict the combined toxicity. - Abstract: The combined toxicities of five insecticides (chlorpyrifos, avermectin, imidacloprid, λ-cyhalothrin, and phoxim), two herbicides (atrazine and butachlor), and a heavy metal (cadmium) have been examined using the acute toxicity test on the earthworm. With a concentration of 2.75 mg/kg being lethal for 50% of the organisms, imidacloprid exhibited the highest acute toxicity toward the earthworm Eisenia fetida. Toxicological interactions of these chemicals in ternary mixtures were studied using the combination-index (CI) equation method. Twenty-one ternary mixtures exhibited various interactive effects, in which 11 combinations showed synergistic effects, four led to dual synergistic/additive behaviors, one exhibited an additive effect, and five showed increasing antagonism within the entire range of effects. The CI method was compared with the classical models of concentration addition and independent action, and it was found that the CI method could accurately predict combined toxicity of the chemicals studied. The predicted synergism in the majority of the mixtures, especially at low-effect levels, might have implications in the real terrestrial environment

Ternary toxicological interactions of insecticides, herbicides, and a heavy metal on the earthworm Eisenia fetida

Energy Technology Data Exchange (ETDEWEB)

Wang, Yanhua [State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control/Key Laboratory for Pesticide Residue Detection of Ministry of Agriculture, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021 (China); Chen, Chen [Key Laboratory of Agro-Product Quality and Safety of Ministry of Agriculture, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081 (China); Qian, Yongzhong, E-mail: qyzcaas@aliyun.com [Key Laboratory of Agro-Product Quality and Safety of Ministry of Agriculture, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081 (China); Zhao, Xueping [State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control/Key Laboratory for Pesticide Residue Detection of Ministry of Agriculture, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021 (China); Wang, Qiang, E-mail: qiangwang2003@vip.sina.com [State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control/Key Laboratory for Pesticide Residue Detection of Ministry of Agriculture, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021 (China)

2015-03-02

Highlights: • The combined toxicity of insecticides, herbicides, and a heavy metal was examined. • Acute earthworm toxicity assays were conducted in twenty-one ternary mixtures. • Synergism predominated in the majority of the mixtures at low effect levels. • Combination index method could more accurately predict the combined toxicity. - Abstract: The combined toxicities of five insecticides (chlorpyrifos, avermectin, imidacloprid, λ-cyhalothrin, and phoxim), two herbicides (atrazine and butachlor), and a heavy metal (cadmium) have been examined using the acute toxicity test on the earthworm. With a concentration of 2.75 mg/kg being lethal for 50% of the organisms, imidacloprid exhibited the highest acute toxicity toward the earthworm Eisenia fetida. Toxicological interactions of these chemicals in ternary mixtures were studied using the combination-index (CI) equation method. Twenty-one ternary mixtures exhibited various interactive effects, in which 11 combinations showed synergistic effects, four led to dual synergistic/additive behaviors, one exhibited an additive effect, and five showed increasing antagonism within the entire range of effects. The CI method was compared with the classical models of concentration addition and independent action, and it was found that the CI method could accurately predict combined toxicity of the chemicals studied. The predicted synergism in the majority of the mixtures, especially at low-effect levels, might have implications in the real terrestrial environment.

Effects of soil properties on copper toxicity to earthworm Eisenia fetida in 15 Chinese soils.

Science.gov (United States)

Duan, Xiongwei; Xu, Meng; Zhou, Youya; Yan, Zengguang; Du, Yanli; Zhang, Lu; Zhang, Chaoyan; Bai, Liping; Nie, Jing; Chen, Guikui; Li, Fasheng

2016-02-01

The bioavailability and toxicity of metals in soil are influenced by a variety of soil properties, and this principle should be recognized in establishing soil environmental quality criteria. In the present study, the uptake and toxicity of Cu to the earthworm Eisenia fetida in 15 Chinese soils with various soil properties were investigated, and regression models for predicting Cu toxicity across soils were developed. The results showed that earthworm survival and body weight change were less sensitive to Cu than earthworm cocoon production. The soil Cu-based median effective concentrations (EC50s) for earthworm cocoon production varied from 27.7 to 383.7 mg kg(-1) among 15 Chinese soils, representing approximately 14-fold variation. Soil cation exchange capacity and organic carbon content were identified as key factors controlling Cu toxicity to earthworm cocoon production, and simple and multiple regression models were developed for predicting Cu toxicity across soils. Tissue Cu-based EC50s for earthworm cocoon production were also calculated and varied from 15.5 to 62.5 mg kg(-1) (4-fold variation). Compared to the soil Cu-based EC50s for cocoon production, the tissue Cu-based EC50s had less variation among soils, indicating that metals in tissue were more relevant to toxicity than metals in soil and hence represented better measurements of bioavailability. Copyright © 2015 Elsevier Ltd. All rights reserved.

Hypotensive and Angiotensin-Converting Enzyme Inhibitory Activities of Eisenia fetida Extract in Spontaneously Hypertensive Rats

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Shumei Mao

2015-01-01

Full Text Available Objectives. This study aimed to investigate the antihypertensive effects of an Eisenia fetida extract (EFE and its possible mechanisms in spontaneously hypertensive rats (SHR rats. Methods. Sixteen-week-old SHR rats and Wistar-Kyoto rats (WKY rats were used in this study. Rats were, respectively, given EFE (EFE group, captopril (captopril group, or phosphate-buffered saline (PBS (normal control group and SHR group for 4 weeks. ACE inhibitory activity of EFE in vitro was determined. The systolic blood pressure (SBP and diastolic blood pressure (DBP were measured using a Rat Tail-Cuff Blood Pressure System. Levels of angiotensin II (Ang II, aldosterone (Ald, and 6-keto-prostaglandin F1 alpha (6-keto-PGF1α in plasma were determined by radioimmunoassay, and serum nitric oxide (NO concentration was measured by Griess reagent systems. Results. EFE had marked ACE inhibitory activity in vitro (IC50 = 2.5 mg/mL. After the 4-week drug management, SHR rats in EFE group and in captopril group had lower SBP and DBP, lower levels of Ang II and Ald, and higher levels of 6-keto-PGF1α and NO than the SHR rats in SHR group. Conclusion. These results indicate that EFE has hypotensive effects in SHR rats and its effects might be associated with its ACE inhibitory activity.

In vitro effects of PCDDs/Fs on NK-like cell activity of Eisenia andrei earthworms

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Hayet Belmeskine

2012-02-01

Full Text Available In this study, we assessed in vitro the effects of PCDD/Fs on the NK-like cell activity in Eisenia andrei earthworms using flow cytometry for analysis. NK-like coelomocytes isolated from E. andrei and used as effectors were exposed to various concentrations of PCDDs/Fs mixture, C1 (6.25x10-3 ng 2378- TCDD/mL, C2 (12.5x10-3 ng 2378-TCDD/mL and C3 (25x10-3 ng 2378-TCDD/mL, before adding them to human tumoral cells (K562 used as targets. We evaluated the percentage of targets lysed by Nk-like cells. The results showed a significant stimulation of the NKlike activity at C3 when PCDD/Fs were not removed from effectors before contact with targets, while no effects were noted when the effectors were washed (PCDD/Fs removed or fixed. Assessment of the viability of the targets (K562, exposed alone and separately from effectors, to the three concentrations of PCDD/Fs, C1, C2 and C3, showed that all these concentrations were cytotoxic for K562. Results suggest that PCDD/Fs concentrations tested in this assay may be considered too low to induce suppressive effects on the immune function such as the NK-like activity in E. andrei earthworms.

Toxicity and toxicokinetics of binary combinations of petroleum hydrocarbon distillates with the earthworm Eisenia andrei.

Science.gov (United States)

Cermak, Janet; Stephenson, Gladys; Birkholz, Detlef; Dixon, D George

2013-04-01

Petroleum hydrocarbons (PHCs) act via narcosis and are expected to have additive toxicity. However, previous work has demonstrated less-than-additive toxicity with PHC distillates and earthworms. A study was initiated to investigate this through toxicity and toxicokinetic studies with the earthworm Eisenia andrei. Three petroleum distillate fractions, F2 (>C10-C16), F3a (>C16-C23), and F3b (>C23-C34), were used in two binary combinations, F2F3a and F3aF3b. In the toxicity study, clean soil was spiked with equitoxic combinations of the two distillates ranging from 0.5 to 2.5 toxic units. In the toxicokinetic study, a binary combination consisting of one concentration of each distillate was used. On a soil concentration basis, the toxicity of the binary combinations of distillates was less than additive. Accumulation of the individual distillates, however, was generally reduced when a second distillate was present, resulting in lower body burden. This is thought to be due to the presence of a nonaqueous-phase liquid at the soil concentrations used. On a tissue concentration basis, toxicity was closer to additive. The results demonstrate that tissue concentrations are the preferred metric for toxicity for earthworms. They also demonstrate that the Canada-wide soil standards based on individual distillates are likely protective. Copyright © 2013 SETAC.

Control of Pathogenicity Root-Knot Nematode (Meloidogyne Javanica by Earthworm Eisenia Feoetida-Based Products in Greenhouse

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M. Rostami

2016-06-01

Full Text Available Introduction: Biocontrol of nematode agents in order to decrease the hazardous impacts of chemical pesticide application including problems of public health and environmental pollution is apriority. In this study, solid (Vermicompost and liquid products (Liquid Vermicompost, Vermiwash and Coelomic fluidof the earthworm species Eisenia fetida were tested against root-knot nematode, Meloidogyne javanica in greenhouse conditions. Materials and Methods: In this study, Solid (Vermicompost and Liquid products(Wormtea, Vermiwash, Coelomic fluid erthworms (Eisenia foetida were tested against Meloidogyne javanica and also the effect of Vermicompost was evaluated on Pathogenicity of various nematode initial inoculum in two stage greenhouse conditions. Earthworm-based products (Vermicompost, Wormtea, Vermiwash and Coelomic fluid were added to tomato pots. Various treatments of liquid as well as solid products and their combination were used in the greenhouse trial. The first Stage greenhouse experiment- Tomato seeds grown in 2 kg sterilized soil. In the treatments having Vermicompost, pots incorporated with 200 gr of this compost homogeneously mixed with soil. After plants reached at two leaf stage, to study the effects of liquid products (Wormtea, Vermiwash, and Coelomic fluid they added to the pots (500cc along with the irrigation water every week and after of 4 leaf stage, 5000 nematode eggs and larva inoculated to the tomato host plants. 90 days after nematode inoculation, plant and nematode growth indices separately measured and compared. The experiment conducted based on completely randomized design having four replicates. The second stage greenhouse experiment- Tomato seeds grown in 2 kg sterilized soil. In the treatments, pots incorporated with 200 gr of this compost homogeneously mixed with soil. After of 4 leaf stage, 0,1000,2000,4000 and 10000 nematode eggs and larva inoculated to the tomato host plants. 90 days after nematode inoculation, plant

PARÁMETROS FISICOQUÍMICOS DURANTE EL ALMACENAMIENTO DE SALCHICHAS ELABORADAS A PARTIR DE TILAPIA ROJA (Oreochromis sp.) PARÂMETROS FISICO-QUÍMICOS DURANTE O ARMAZENAMENTO DE SALSICHAS FEITAS DE TILAPIA VERMELHA (Oreochromis sp.) PHYSICOCHEMICAL PARAMETERS DURING STORAGE OF SAUSAGES MADE FROM RED TILAPIA (Oreochromis sp.)

OpenAIRE

JOSÉ IGOR HLEAP Z.; VIVIANA ANDREA VELASCO A.

2012-01-01

El crecimiento de la acuicultura de la tilapia roja (Oreochromis sp.) en Colombia ha permitido desarrollar productos alimenticios de mayor valor agregado. Uno de estos productos, son las salchichas tipo Frankfurt. El objetivo del presente trabajo fue, analizar los parámetros fisicoquímicos que afectan la vida de anaquel de las salchichas, elaboradas con adición de almidón de sagú (Maranthaarundinacea) como material ligante o extendedor. El diseño planteado permitió evaluar las salchichas empa...

Vermicomposting of source-separated human faeces by Eisenia fetida: effect of stocking density on feed consumption rate, growth characteristics and vermicompost production.

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Yadav, Kunwar D; Tare, Vinod; Ahammed, M Mansoor

2011-06-01

The main objective of the present study was to determine the optimum stocking density for feed consumption rate, biomass growth and reproduction of earthworm Eisenia fetida as well as determining and characterising vermicompost quantity and product, respectively, during vermicomposting of source-separated human faeces. For this, a number of experiments spanning up to 3 months were conducted using soil and vermicompost as support materials. Stocking density in the range of 0.25-5.00 kg/m(2) was employed in different tests. The results showed that 0.40-0.45 kg-feed/kg-worm/day was the maximum feed consumption rate by E. fetida in human faeces. The optimum stocking densities were 3.00 kg/m(2) for bioconversion of human faeces to vermicompost, and 0.50 kg/m(2) for earthworm biomass growth and reproduction. Copyright © 2011 Elsevier Ltd. All rights reserved.

Enzymatic dynamics into the Eisenia fetida (Savigny, 1826) gut during vermicomposting of coffee husk and market waste in a tropical environment.

Science.gov (United States)

Ordoñez-Arévalo, Berenice; Guillén-Navarro, Karina; Huerta, Esperanza; Cuevas, Raúl; Calixto-Romo, M Angeles

2018-01-01

Epigeic worms modify microbial communities through their digestive processes, thereby influencing the decomposition of organic matter in vermicomposting systems. Nevertheless, the enzyme dynamics within the gut of tropically adapted earthworms is unknown, and the enzymes involved have not been simultaneously studied. The activities of 19 hydrolytic enzymes within three different sections of the intestine of Eisenia fetida were determined over a fasting period and at 24 h and 30, 60, and 90 days of vermicomposting, and data were evaluated by multivariate analyses. There were found positive correlations between the maximal activity of glycosyl hydrolases and one esterase with the anterior intestine (coincident with the reduction of hemicellulose in the substrate) and the activity of the protease α-chymotrypsin with posterior intestine. The results suggest that activities of enzymes change in a coordinated manner within each gut section, probably influenced by selective microbial enzyme enrichment and by the availability of nutrients throughout vermicomposting.

EVALUACIÓN DEL ENSILAJE DE VÍSCERAS DE TILAPIA ROJA (Oreochromis spp EN ALIMENTACIÓN DE POLLOS DE ENGORDE

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GILMA M. GOMEZ N.

2014-06-01

Full Text Available Los mayores costos de producción en la avicultura, son debidos a la alimentación, de ahí la importancia de generar estrategias encaminadas a su reducción, por ello, se planteó como objetivo, evaluar el efecto de la inclusión del ensilaje biológico de vísceras de tilapia roja (Oreochromis spp en la alimentación de pollos de engorde, durante la etapa de iniciación. Para ello se utilizó un diseño completamente al azar, con cuatro tratamientos, cuatro réplicas por tratamiento y 8 pollos por repetición. Los niveles de inclusión empleados fueron 0%, 10%, 20% y 30%. Se tomaron como variables de respuesta, el consumo de alimento, ganancia de peso, conversión alimenticia y su respectivo análisis económico. En la etapa de iniciación, solamente se presentaron diferencias estadísticas (p>0,05, para la variable consumo de alimento, mientras que en la etapa de finalización, donde no se incluyo ensilaje, no se presentaron diferencias significativas. Respecto al análisis económico, se determino que en la medida que se aumenta el nivel de inclusión de ensilaje, se reducen los costos de producción, hasta en un 22,2%, para el caso del tratamiento 3.

Bioaccumulation of perfluoroalkyl acids by earthworms (Eisenia fetida) exposed to contaminated soils.

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Rich, Courtney D; Blaine, Andrea C; Hundal, Lakhwinder; Higgins, Christopher P

2015-01-20

The presence of perfluoroalkyl acids (PFAAs) in biosolids-amended and aqueous film-forming foam (AFFF)-impacted soils results in two potential pathways for movement of these environmental contaminants into terrestrial foodwebs. Uptake of PFAAs by earthworms (Eisenia fetida) exposed to unspiked soils with varying levels of PFAAs (a control soil, an industrially impacted biosolids-amended soil, a municipal biosolids-amended soil, and two AFFF-impacted soils) was measured. Standard 28 day exposure experiments were conducted in each soil, and measurements taken at additional time points in the municipal soil were used to model the kinetics of uptake. Uptake and elimination rates and modeling suggested that steady state bioaccumulation was reached within 28 days of exposure for all PFAAs. The highest concentrations in the earthworms were for perfluorooctane sulfonate (PFOS) in the AFFF-impacted Soil A (2160 ng/g) and perfluorododecanoate (PFDoA) in the industrially impacted soil (737 ng/g). Wet-weight (ww) and organic carbon (OC)-based biota soil accumulation factors (BSAFs) for the earthworms were calculated after 28 days of exposure for all five soils. The highest BSAF in the industrially impacted soil was for PFDoA (0.42 goc/gww,worm). Bioaccumulation factors (BAFs, dry-weight-basis, dw) were also calculated at 28 days for each of the soils. With the exception of the control soil and perfluorodecanoate (PFDA) in the industrially impacted soil, all BAF values were above unity, with the highest being for perfluorohexanesulfonate (PFHxS) in the AFFF-impacted Soil A (139 gdw,soil/gdw,worm). BSAFs and BAFs increased with increasing chain length for the perfluorocarboxylates (PFCAs) and decreased with increasing chain length for the perfluoroalkyl sulfonates (PFSAs). The results indicate that PFAA bioaccumulation into earthworms depends on soil concentrations, soil characteristics, analyte, and duration of exposure, and that accumulation into earthworms may be a potential

Evaluación por rendimiento de 12 genotipos promisorios de fríjol voluble (Phaseolus vulgaris L. tipo Bola roja y Reventón para las zonas frías de Colombia

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López Jesús Edgardo

2006-12-01

Full Text Available

El fríjol común (Phaseolus vulgaris L. es un alimento básico en la región Andina por ser una fuente rica en proteína y de bajo costo. La investigación para incrementar rendimientos en esta leguminosa es una opción para mejorar la competitividad en el mercado mundial. El objetivo principal de este trabajo fue evaluar por rendimiento los genotipos promisorios de fríjol voluble, tipos Bola roja y Reventón, para las zonas frías de Colombia mediante el análisis de sendero. Se realizó un diseño de bloques completos al azar con tres réplicas para evaluar 10 genotipos promisorios de fríjol voluble. El análisis de sendero para el rendimiento por planta y las correlaciones entre el rendimiento y sus componentes mostraron que el carácter número de vainas por planta es el de mayor importancia sobre la determinación del rendimiento, en comparación con los caracteres peso de 100 semillas y número de semillas por vaina, tanto en los genotipos de fríjol voluble tipo Bola roja como tipo Reventón. 

Respuesta de niveles crecientes de NK en la producción de cebolla (Allium cepa L. var. “Roja Arequipeña”

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Julio Estuardo Amaya Robles

2013-01-01

Full Text Available Con el objetivo de determinar la influencia de niveles crecientes de nitrógeno y potasio en la producción de cebolla, fue conducido un experimento en el Campo Experimental del Instituto de Investigación y Transferencia Agropecuaria ubicado en el sector Pampas de San Juan , Laredo, La Libertad (Perú . El diseño experimental utilizado fue de Bloques Completos al Azar con arreglo factorial 3x 4 con tres repeticiones. Los tratamientos resultaron de las combinaciones 60, 120, 180 y 240, kg de N . ha - 1 ; y 40, 80 y 160 kg de K 2 O ha - 1 , utilizándose como dosis única 80 kg de P 2 O 5. ha - 1 y semilla certificada de la var. “Roja Arequipeña”. Se determinó e l rendimiento de este cultivo en función de los tratamientos, obteniéndose respuestas lineares crecientes de 1,010 y 1,006 g - 1 promedio de 10 bulbos, con las combinaciones de N 120 xK 80 y N 60 x K 80; y una respuesta cuadrática a N con la dosis de 120 kg.ha - 1 con la cual se promovió la mayor producción de bulbos de cebolla.

Red worm behavior (Eisenia spp. in vermicomposting systems of organic residues

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Mamani-Mamani Gladys

2012-08-01

Full Text Available This work evaluates the behavior of the vermicomposting red worm (Eisenia spp. in two environments (greenhouse and unsheltered and with two solid organic substrates (cow manure=CM and kitchen waste=KW in the zone of Carmen Pampa, Nor Yungas Province, Department of La Paz – Bolivia, using a factorial design with two factors and three repetitions (ANOVA analysis. The largest number of cocoons was found in the greenhouse treatment, with 64 in CM and 41 in KW. Cocoon viability was also greatest in the greenhouse treatment, at 100% for CM and 96% for KW. Similarly, the greatest number of worms hatched from cocoons was in the greenhouse treatment, with 2 immature worms for CM and 3.5 for KW, and the greatest number of immature worms was registered in the greenhouse treatment with 123 individuals in CM and 16 in KW. The lowest mortality rate due to environment was in the greenhouse treatment, with 3.90% mortality with CM and 88.64% with KW. The greatest number of mature worms (with clitella was in the unsheltered treatment, with 15 in CM and 21 in KW. The greatest biomass of immature worms was found in the greenhouse treatment, with 1.41 g of worms for CM and 0.185 g for KW; however, the greatest biomass of mature worms was in the KW treatment, with 7.98 g for the greenhouse treatment and 6.93 g for the unsheltered treatment. The phytotoxicity from CM vermicompost in the two environments was lowest, exhibiting a 66.6% rate of germination, and the opposite was true for KW, which was the most toxic in both environments. Macronutrient content in vermicompost obtained was: nitrogen at 2.45% and 2.31%, phosphorus at 500 mg kg-1 and 220 mg kg-1 and potassium at 27.43 and 2.76 cmol(+ kg-1 of dry substrate in KW and CM respectively.

Uptake of hexanitrohexaazaisowurtzitane (CL-20) by the earthworm Eisenia fetida through dermal contact

International Nuclear Information System (INIS)

Gong Ping; Escalon, B. Lynn; Hayes, Charolett A.; Perkins, Edward J.

2008-01-01

The explosive compound hexanitrohexaazaisowurtzitane (CL-20) has been shown to cause both lethal and sublethal (reproductive and neurotoxic) effects in exposed oligochaetes. However, whether worms take up CL-20 and how much CL-20 enters worm bodies leading to toxicity (e.g., lethality) remain to be determined. In the present study, we used high performance liquid chromatography (HPLC) and radiolabeled tracer methods to investigate the CL-20 uptake in the whole worm body after contact exposures. Worms (Eisenia fetida) were exposed to filter paper spiked with non-radioactive or [U- 14 C]-labeled CL-20 for 1-3 d. The radiolabeled tracer method allowed us to detect the parent compound and transformation products in worms exposed to as low as 0.04 μg CL-20 cm -2 of filter paper. The HPLC method without radiolabeled tracer was far less sensitive with a detection limit of 2.17 μg CL-20 cm -2 . Using the radiolabeled tracer, we were able to demonstrate that the worm body concentration linearly correlated to the filter paper concentration ≤ 0.34 μg cm -2 (r = 0.94) if no breakdown products are assumed. At higher concentrations, the body concentration increased slowly and saturated at around 11 μg g -1 dry mass resulting in an estimated lethal critical body burden of 10-15 μg CL-20 g -1 dry mass. These findings demonstrate that CL-20 or potential transformation products are taken into the earthworm body through dermal contact. This information should prove valuable in assessing the bioaccumulation potential and ecological risks of CL-20

Uptake of hexanitrohexaazaisowurtzitane (CL-20) by the earthworm Eisenia fetida through dermal contact

Energy Technology Data Exchange (ETDEWEB)

Gong Ping [SpecPro, 3909 Halls Ferry Road, Vicksburg, MS 39180 (United States)], E-mail: ping.gong@erdc.usace.army.mil; Escalon, B. Lynn; Hayes, Charolett A. [SpecPro, 3909 Halls Ferry Road, Vicksburg, MS 39180 (United States); Perkins, Edward J. [Environmental Laboratory, US Army Engineer Research and Development Center, 3909 Halls Ferry Road, Vicksburg, MS 39180 (United States)

2008-02-01

The explosive compound hexanitrohexaazaisowurtzitane (CL-20) has been shown to cause both lethal and sublethal (reproductive and neurotoxic) effects in exposed oligochaetes. However, whether worms take up CL-20 and how much CL-20 enters worm bodies leading to toxicity (e.g., lethality) remain to be determined. In the present study, we used high performance liquid chromatography (HPLC) and radiolabeled tracer methods to investigate the CL-20 uptake in the whole worm body after contact exposures. Worms (Eisenia fetida) were exposed to filter paper spiked with non-radioactive or [U-{sup 14}C]-labeled CL-20 for 1-3 d. The radiolabeled tracer method allowed us to detect the parent compound and transformation products in worms exposed to as low as 0.04 {mu}g CL-20 cm{sup -2} of filter paper. The HPLC method without radiolabeled tracer was far less sensitive with a detection limit of 2.17 {mu}g CL-20 cm{sup -2}. Using the radiolabeled tracer, we were able to demonstrate that the worm body concentration linearly correlated to the filter paper concentration {<=} 0.34 {mu}g cm{sup -2} (r = 0.94) if no breakdown products are assumed. At higher concentrations, the body concentration increased slowly and saturated at around 11 {mu}g g{sup -1} dry mass resulting in an estimated lethal critical body burden of 10-15 {mu}g CL-20 g{sup -1} dry mass. These findings demonstrate that CL-20 or potential transformation products are taken into the earthworm body through dermal contact. This information should prove valuable in assessing the bioaccumulation potential and ecological risks of CL-20.

Effects of Cry1Ab Transgenic Maize on Lifecycle and Biomarker Responses of the Earthworm, Eisenia Andrei

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Mark Maboeta

2012-12-01

Full Text Available A 28-day study was conducted to determine the effects of the Bacillus thuringiensis Cry1Ab toxin on the earthworm Eisenia andrei. Previously, investigations have been limited to life-cycle level effects of this protein on earthworms, and mostly on E. fetida. In this study several endpoints were compared which included biomass changes, cocoon production, hatching success, a cellular metal-stress biomarker (Neutral Red Retention Time; NRRT and potential genotoxic effects in terms of Randomly Amplified Polymorphic DNA sequences (RAPDs. NRRT results indicated no differences between treatments (p > 0.36, and NRRT remained the same for both treatments at different times during the experiment (p = 0.18. Likewise, no significant differences were found for cocoon production (p = 0.32 or hatching success (p = 0.29. Conversely, biomass data indicated a significant difference between the control treatment and the Bt treatment from the second week onwards (p < 0.001, with the Bt treatment losing significantly more weight than the isoline treatment. Possible confounding factors were identified that might have affected the differences in weight loss between groups. From the RAPD profiles no conclusive data were obtained that could link observed genetic variation to exposure of E. andrei to Cry1Ab proteins produced by Bt maize.

Influence of activated carbon amendment on the accumulation and elimination of PCBs in the earthworm Eisenia fetida

Energy Technology Data Exchange (ETDEWEB)

Paul, Piuly [Department of Civil and Environmental Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MA 21250 (United States); Ghosh, Upal, E-mail: ughosh@umbc.edu [Department of Civil and Environmental Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MA 21250 (United States)

2011-12-15

In this study we investigated the use of activated carbon (AC) as a soil amendment for reducing bioavailability of polychlorinated biphenyls (PCBs) to the earthworm Eisenia fetida. Artificial soil was contaminated with PCBs and used in bioaccumulation experiments fresh or after aging for 19 months. PCB bioaccumulation in earthworms was reduced by 68% when AC was placed as a layer without mixing and by 94% when AC was manually mixed into the soil. Aging of the same AC mixed soil for 19 months resulted in an overall reduction of 99% in PCB biouptake. AC-treated aged soil also showed two orders of magnitude lower equilibrium aqueous concentrations of PCBs compared to untreated aged soils. The findings from this study indicate that application of engineered sorbents like AC to PCB impacted soils may greatly reduce PCB uptake at the base of the terrestrial food chain. - Graphical abstract: Display Omitted Highlights: > Activated carbon studied as a sorbent for PCB impacted unsaturated soil. > Bioaccumulation of PCB greatly reduced in earthworms after carbon amendment. > Aging of the activated carbon amended soil further reduced bioaccumulation. > Activated carbon can be used for in-situ stabilization of PCB impacted soil. - Addition of activated carbon to PCB impacted soil reduces equilibrium aqueous concentrations and uptake at the base of the terrestrial food chain.

The effects of the insecticide lambda-Cyhalothrin on the earthworm Eisenia fetida under experimental conditions of tropical and temperate regions.

Science.gov (United States)

Garcia, Marcos; Scheffczyk, Adam; Garcia, Terezinha; Römbke, Jörg

2011-02-01

Plant Protection Products can affect soil organisms and thus might have negative impacts on soil functions. Little research has been performed on their impact on tropical soils. Therefore, the effects of the insecticide lambda-Cyhalothrin on earthworms were evaluated in acute and chronic laboratory tests modified for tropical conditions, i.e. at selected temperatures (20 and 28°C) and with two strains (temperate and tropical) of the compost worm Eisenia fetida. The insecticide was spiked in two natural soils, in OECD artificial soil and a newly developed tropical artificial soil. The effects of lambda-Cyhalothrin did rarely vary in the same soil at tropical (LC50: 68.5-229 mg a.i./kg dry weight (DW); EC50: 54.2-60.2 mg a.i./kg DW) and temperate (LC50: 99.8-140 mg a.i./kg DW; EC50: 37.4-44.5 mg a.i./kg DW) temperatures. In tests with tropical soils and high temperature, effect values differed by up to a factor of ten. Copyright © 2010 Elsevier Ltd. All rights reserved.

Eisenia fetida avoidance behavior as a tool for assessing the efficiency of remediation of Pb, Zn and Cd polluted soil

International Nuclear Information System (INIS)

Udovic, Metka; Lestan, Domen

2010-01-01

Remediation by means of soil leaching with ethylenediaminetetraacetic acid (EDTA) is capable of extracting the most labile soil fractions, leaving the residual metals in biologically non-available forms. We evaluated the feasibility of the standardized earthworm (Eisenia fetida) avoidance test for assessing the efficiency of soil remediation of Pb, Zn and Cd polluted soil. Chemical extraction tests (six-step sequential extraction, toxicity characteristic leaching procedure, physiologically based extraction test, diethylenediaminepentaacetic acid extraction) indicated that the mobility, oral bioaccessibility and phytoavailability of Pb, Zn and Cd were consistently reduced. However, the avoidance test showed no significant avoidance of polluted soil in favor of that which had been remediated. Pb, Zn and Cd accumulation in E. fetida mirrored the decreasing pattern of metal potential bioavailability gained by leaching the soil with increasing EDTA concentrations. The calculated bioaccumulation factors indicated the possibility of underestimating the metal bioavailability in soil using chemical extraction tests. - Tests with indicator organisms should be used for a more meaningful and holistic assessment of metal biological availability in polluted and remediated soil.

Nutrient Status of Vermicompost of Urban Green Waste Processed by Three Earthworm Species—Eisenia fetida, Eudrilus eugeniae, and Perionyx excavatus

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Swati Pattnaik

2010-01-01

Full Text Available Major nutrient status of vermicompost of vegetable market waste (MW and floral waste (FW processed by three species of earthworms namely, Eudrilus eugeniae, Eisenia fetida, and Perionyx excavatus and its simple compost were assessed across different periods in relation to their respective initiative substrates. Their physical parameters—temperature, moisture, pH, and electrical conductivity—were also recorded. The nutrients—nitrogen, phosphorus, potassium, calcium, and magnesium—increased in the vermicompost and compost while the organic carbon, C/N and C/P ratios decreased as the composting process progressed from 0 to 15, 30, 45, and 60 days. The nutrient statuses of vermicomposts of all earthworm species produced from both the wastes were more than that of the compost and that of their respective substrates. Moreover, the vermicompost produced by E. eugeniae possessed higher nutrient contents than that of E. fetida, P. excavatus, and compost. The MW showed higher nutrient contents than the FW. Thus, vermicomposting is the paramount approach of nutrient recovery of urban green waste.

Eisenia fetida avoidance behavior as a tool for assessing the efficiency of remediation of Pb, Zn and Cd polluted soil

Energy Technology Data Exchange (ETDEWEB)

Udovic, Metka [Centre for Soil and Environmental Science, Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, Sl-1000 Ljubljana (Slovenia); Lestan, Domen, E-mail: domen.lestan@bf.uni-lj.s [Centre for Soil and Environmental Science, Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, Sl-1000 Ljubljana (Slovenia)

2010-08-15

Remediation by means of soil leaching with ethylenediaminetetraacetic acid (EDTA) is capable of extracting the most labile soil fractions, leaving the residual metals in biologically non-available forms. We evaluated the feasibility of the standardized earthworm (Eisenia fetida) avoidance test for assessing the efficiency of soil remediation of Pb, Zn and Cd polluted soil. Chemical extraction tests (six-step sequential extraction, toxicity characteristic leaching procedure, physiologically based extraction test, diethylenediaminepentaacetic acid extraction) indicated that the mobility, oral bioaccessibility and phytoavailability of Pb, Zn and Cd were consistently reduced. However, the avoidance test showed no significant avoidance of polluted soil in favor of that which had been remediated. Pb, Zn and Cd accumulation in E. fetida mirrored the decreasing pattern of metal potential bioavailability gained by leaching the soil with increasing EDTA concentrations. The calculated bioaccumulation factors indicated the possibility of underestimating the metal bioavailability in soil using chemical extraction tests. - Tests with indicator organisms should be used for a more meaningful and holistic assessment of metal biological availability in polluted and remediated soil.

Cloning, analysis and functional annotation of expressed sequence tags from the Earthworm Eisenia fetida

Science.gov (United States)

Pirooznia, Mehdi; Gong, Ping; Guan, Xin; Inouye, Laura S; Yang, Kuan; Perkins, Edward J; Deng, Youping

2007-01-01

Background Eisenia fetida, commonly known as red wiggler or compost worm, belongs to the Lumbricidae family of the Annelida phylum. Little is known about its genome sequence although it has been extensively used as a test organism in terrestrial ecotoxicology. In order to understand its gene expression response to environmental contaminants, we cloned 4032 cDNAs or expressed sequence tags (ESTs) from two E. fetida libraries enriched with genes responsive to ten ordnance related compounds using suppressive subtractive hybridization-PCR. Results A total of 3144 good quality ESTs (GenBank dbEST accession number EH669363–EH672369 and EL515444–EL515580) were obtained from the raw clone sequences after cleaning. Clustering analysis yielded 2231 unique sequences including 448 contigs (from 1361 ESTs) and 1783 singletons. Comparative genomic analysis showed that 743 or 33% of the unique sequences shared high similarity with existing genes in the GenBank nr database. Provisional function annotation assigned 830 Gene Ontology terms to 517 unique sequences based on their homology with the annotated genomes of four model organisms Drosophila melanogaster, Mus musculus, Saccharomyces cerevisiae, and Caenorhabditis elegans. Seven percent of the unique sequences were further mapped to 99 Kyoto Encyclopedia of Genes and Genomes pathways based on their matching Enzyme Commission numbers. All the information is stored and retrievable at a highly performed, web-based and user-friendly relational database called EST model database or ESTMD version 2. Conclusion The ESTMD containing the sequence and annotation information of 4032 E. fetida ESTs is publicly accessible at . PMID:18047730

Biomarker responses in earthworms (Eisenia fetida) to soils contaminated with di-n-butyl phthalates.

Science.gov (United States)

Du, Li; Li, Guangde; Liu, Mingming; Li, Yanqiang; Yin, Suzhen; Zhao, Jie

2015-03-01

Di-n-butyl phthalates (DBP) are recognized as ubiquitous contaminants in soil and adversely impact the health of organisms. Changes in the activity of antioxidant enzymes and levels of glutathione-S-transferase (GST), glutathione (GSH), and malondialdehyde (MDA) were used as biomarkers to evaluate the impact of DBP on earthworms (Eisenia fetida) after exposure to DBP for 28 days. DBP was added to artificial soil in the amounts of 0, 5, 10, 50, and 100 mg kg(-1) of soil. Earthworm tissues exposed to each treatment were collected on the 7th, 14th, 21st, and 28th day of the treatment. We found that superoxide dismutase (SOD) and catalase (CAT) levels were significantly inhibited in the 100 mg kg(-1) treatment group on day 28. After 21 days of treatment, GST activity in 10-50 mg kg(-1) treatment groups was markedly stimulated compared to the control group. MDA content in treatment groups was higher than in the control group throughout the exposure time, suggesting that DBP may lead to lipid peroxidation (LPO) in cells. GSH content increased in the treatment group that received 50 mg kg(-1) DBP from 7 days of exposure to 28 days. These results suggest that DBP induces serious oxidative damage on earthworms and induce the formation of reactive oxygen species (ROS) in earthworms. However, DBP concentration in current agricultural soil in China will not constitute any threat to the earthworm or other animals in the soil.

Toxicity effects of di-(2-ethylhexyl phthalate to Eisenia fetida at enzyme, cellular and genetic levels.

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Tingting Ma

Full Text Available Di-(2-ethylhexyl phthalate (DEHP is a dominant phthalic acid ester (PAE that has aroused public concern due to its resistance to degradation and its toxicity as an endocrine-disrupting compound. Effects of different concentrations of DEHP on Eisenia fetida in spiked natural soil have been studied in the body of the earthworm by means of soil cultivation tests 7, 14, 21 and 28 days after exposure. The results indicated that, in general, superoxide dismutase (SOD activity, malondialdehyde (MDA content, metallothionein (MT content, the expression of heat shock protein 70 (HSP 70 and all the tested geno-toxicity parameters are promoted as time elapses and with increasing concentration of DEHP. However, peroxidase (POD activity, neutral red retention time (NRRT and mitochondrial membrane potential difference values were found to decrease even at a low concentration of DEHP of 1 mg kg-1 soil (p<0.05. Clear toxic effects of DEHP on E. fetida have been generally recognized by means of the disturbance of antioxidant enzyme activity/content and critical proteins, cell membrane and organelle disorder and DNA damage estimated by length of tail, tail DNA ratio, and tail moment parameters. A concentration of DEHP of 3 mg kg-1 may be recommended as a precaution against the potential risk of PAEs in soils and for indicating suitable threshold values for other soil animals and soil micro-organisms.

Fucofuroeckol-A from Eisenia bicyclis Inhibits Inflammation in Lipopolysaccharide-Induced Mouse Macrophages via Downregulation of the MAPK/NF-κB Signaling Pathway

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Sang-Hoon Lee

2016-01-01

Full Text Available Fucofuroeckol-A (FF isolated from an edible perennial brown seaweed Eisenia bicyclis was shown to be potent anti-inflammatory agents. FF suppressed the production of nitric oxide (NO and prostaglandin E2 (PGE2 and the expression of inducible nitric oxide synthase and cyclooxygenase-2 dose dependently in lipopolysaccharide- (LPS- induced RAW 264.7 mouse macrophages. An enzyme-linked immunosorbent assay and cytometric bead array assay demonstrated that FF significantly reduced the production of proinflammatory cytokines, such as interleukin-6 and tumor necrosis factor-α, and that of the monocyte chemoattractant protein-1. Moreover, FF reduced the activation of nuclear factor κB (NF-κB and mitogen-activated protein kinases (MAPKs. These results strongly suggest that the inhibitory effects of fucofuroeckol-A from E. bicyclis on LPS-induced NO and PGE2 production might be due to the suppression of the NF-κB and MAPK signaling pathway.

Soil pH effects on the comparative toxicity of dissolved zinc, non-nano and nano ZnO to the earthworm Eisenia fetida.

Science.gov (United States)

Heggelund, Laura R; Diez-Ortiz, Maria; Lofts, Stephen; Lahive, Elma; Jurkschat, Kerstin; Wojnarowicz, Jacek; Cedergreen, Nina; Spurgeon, David; Svendsen, Claus

2014-08-01

To determine how soil properties influence nanoparticle (NP) fate, bioavailability and toxicity, this study compared the toxicity of nano zinc oxide (ZnO NPs), non-nano ZnO and ionic ZnCl2 to the earthworm Eisenia fetida in a natural soil at three pH levels. NP characterisation indicated that reaction with the soil media greatly controls ZnO properties. Three main conclusions were drawn. First that Zn toxicity, especially for reproduction, was influenced by pH for all Zn forms. This can be linked to the influence of pH on Zn dissolution. Secondly, that ZnO fate, toxicity and bioaccumulation were similar (including relationships with pH) for both ZnO forms, indicating the absence of NP-specific effects. Finally, earthworm Zn concentrations were higher in worms exposed to ZnO compared to ZnCl2, despite the greater toxicity of the ionic form. This observation suggests the importance of considering the relationship between uptake and toxicity in nanotoxicology studies.

Potential utilization of bagasse as feed material for earthworm Eisenia fetida and production of vermicompost.

Science.gov (United States)

Bhat, Sartaj Ahmad; Singh, Jaswinder; Vig, Adarsh Pal

2015-01-01

In the present work bagasse (B) i.e waste of the sugar industry, was fed to Eisenia fetida with cattle dung (CD) support as feed material at various ratios (waste: CD) of 0:100 (B0), 25:75 (B25), 50:50 (B50), 75:25 (B75) and 100:0 (B100) on dry weight basis. Co-composting with cattle dung helped to improve their acceptability for E. fetida and also improved physico-chemical characteristics. Best appropriate ratio for survival, maximum growth and population buildup of E. fetida was determined by observing population buildup, growth rate, biomass, mortality and cocoon formation. Minimum mortality and highest population size of worms was observed in 50:50 (B50) ratio. Increasing concentrations of wastes significantly affected the growth and reproduction of worms. Nutrients like nitrogen, phosphorus and sodium increased from pre-vermicompost to post-vermicompost, while organic carbon, and C:N ratio decreased in all the end products of post-vermicomposting. Heavy metals decreased significantly from initial except zinc, iron and manganese which increased significantly. Scanning electron microscopy (SEM) was used to recognize the changes in texture in the pre and post-vermicomposted samples. The post-vermicomposted ratios in the presence of earthworms validate more surface changes that prove to be good manure. The results observed from the present study indicated that the earthworm E. fetida was able to change bagasse waste into nutrient-rich manure and thus play a major role in industrial waste management.

Effect of time and mode of depuration on tissue copper concentrations of the earthworms Eisenia andrei, Lumbricus rubellus and Lumbricus terrestris

International Nuclear Information System (INIS)

Arnold, R.E.; Hodson, M.E.

2007-01-01

Eisenia andrei, Lumbricus rubellus and Lumbricus terrestris were exposed to 250, 250 and 350 mg kg -1 Cu respectively in Cu(NO 3 ) 2(aq) amended soil for 28 d. Earthworms were then depurated for 24 to 72 h, digested and analysed for Cu and Ti or, subsequent to depuration were dissected to remove any remaining soil particles from the alimentary canal and then digested and analysed. This latter treatment proved impossible for E. andrei due to its small size. Regardless of depuration time, soil particles were retained in the alimentary canal of L. rubellus and L. terrestris. Tissue concentration determinations indicate that E. andrei should be depurated for 24 h, L. rubellus for 48 h and L. terrestris should be dissected. Ti was bioaccumulated and therefore could not be used as an inert tracer to determine mass of retained soil. Calculations indicate that after 28 d earthworms were still absorbing Cu from soil. - Even after 72 h depuration earthworms retain soil particles in their alimentary canal that can bias tissue concentration determinations

Toxic responses of cytochrome P450 sub-enzyme activities to heavy metals exposure in soil and correlation with their bioaccumulation in Eisenia fetida.

Science.gov (United States)

Cao, Xiufeng; Bi, Ran; Song, Yufang

2017-10-01

The dose- and time- dependent responses of cytochrome P450 (CYP) sub-enzyme activities to heavy metals in soil, and the relationships between biomarker responses and metal bioaccumulation in Eisenia fetida were evaluated. Earthworms were exposed to soils spiked with increasing doses of Cd, Cu, Pb or Zn for 21 d. Results demonstrated that EROD and CYP3A4 activities responded significantly with increasing dose and exposure duration. EROD activity significantly (P metal burdens had significant correlation with the total metal concentrations in soil (P metal concentration in soil. The order of metal bioavailability to E. fetida was Cd > Zn > Cu > Pb. CYP3A4 activity in Pb-exposed earthworms had a significant correlation with the accumulated metal (P heavy metals exposure, and we also concluded that different biomarkers with multiple durations could be conducted in the eco-toxicological diagnosis of soil pollution. Copyright © 2017 Elsevier Inc. All rights reserved.

Availability of polycyclic aromatic hydrocarbons to earthworms (Eisenia andrei, Oligochaeta) in field-polluted soils and soil-sediment mixtures.

Science.gov (United States)

Jager, Tjalling; Baerselman, Rob; Dijkman, Ellen; de Groot, Arthur C; Hogendoorn, Elbert A; de Jong, Ad; Kruitbosch, Jantien A W; Peijnenburg, Willie J G M

2003-04-01

The bioavailability of polycyclic aromatic hydrocarbons (PAHs) for earthworms (Eisenia andrei) was experimentally determined in seven field-polluted soils and 15 soil-sediment mixtures. The pore-water concentration of most PAHs was higher than predicted. However, most of the compound was associated with dissolved organic carbon (DOC) and not directly available for uptake by earthworms. The apparent sorption could be reasonably predicted on the basis of interactions with DOC; however, the biota-soil accumulation factors (BSAFs) for earthworms were up to two orders of magnitude lower than predicted by equilibrium partitioning. The large variability between sites was not fully explained by differences in sorption. Experimental results indicate that the pool of freely dissolved PAHs in the pore water became partially depleted because of uptake by the earthworms and that bioaccumulation is thus also influenced by the kinetics of PAH desorption and mass transport. A pilot study with Lumbricus rubellus showed that steady-state body residues were well correlated to E. andrei. Current results show that depositing dredge spoil on land may lead to increased bioavailability of the lower-molecular-weight PAHs. However, risk assessment can conservatively rely on equilibrium partitioning, but accurate prediction requires quantification of the kinetics of bioavailability.

Effects of Biosolids at Varying Rates on Earthworms (Eisenia fetida and Springtails (Folsomia candida

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N. Artuso

2011-01-01

Full Text Available Land spreading is a major option internationally for the disposal/use of treated sewage sludge (biosolids, but effects of this practice on soil organisms are largely unknown. This study investigated the effects of biosolids on two soil invertebrate species, earthworms (Eisenia fetida and Collembola (Folsomia candida, in laboratory tests. Five biosolids from different sewage works were assessed at rates equivalent to 0, 2, 5, 10, and 20 t ha−1. Biosolids applied at 2 and 5 t ha−1 did not cause mortality of adult earthworms but did at 10 and 20 t ha−1. At 5, 10 and 20 t ha−1, all biosolids had significantly fewer juvenile worms relative to controls. Increasing the rates from 2 to 10 t ha−1 did not impact on the number of adult Collembola, but at 20 t ha−1 there were significantly fewer adults. There were significantly fewer juvenile Collembola recorded for biosolids applied at the 2 t ha−1 when compared with controls, and also when biosolids were applied at 5, 10, and 20 t ha−1 relative to 2 t ha−1. Some significant difference between biosolids were observed, but generally, negative effects were not related to heavy metal concentrations in biosolids. It is recommended that possible detrimental mechanisms (e.g., ammonia production, lack of oxygen be investigated in future work. It is concluded that biosolids, applied at legal, low rates (about 2 t ha−1 are unlikely to be detrimental to earthworms or adult Collembola but can be detrimental to Collembola reproduction.

Influence of activated carbon amendment on the accumulation and elimination of PCBs in the earthworm Eisenia fetida

International Nuclear Information System (INIS)

Paul, Piuly; Ghosh, Upal

2011-01-01

In this study we investigated the use of activated carbon (AC) as a soil amendment for reducing bioavailability of polychlorinated biphenyls (PCBs) to the earthworm Eisenia fetida. Artificial soil was contaminated with PCBs and used in bioaccumulation experiments fresh or after aging for 19 months. PCB bioaccumulation in earthworms was reduced by 68% when AC was placed as a layer without mixing and by 94% when AC was manually mixed into the soil. Aging of the same AC mixed soil for 19 months resulted in an overall reduction of 99% in PCB biouptake. AC-treated aged soil also showed two orders of magnitude lower equilibrium aqueous concentrations of PCBs compared to untreated aged soils. The findings from this study indicate that application of engineered sorbents like AC to PCB impacted soils may greatly reduce PCB uptake at the base of the terrestrial food chain. - Graphical abstract: Display Omitted Highlights: → Activated carbon studied as a sorbent for PCB impacted unsaturated soil. → Bioaccumulation of PCB greatly reduced in earthworms after carbon amendment. → Aging of the activated carbon amended soil further reduced bioaccumulation. → Activated carbon can be used for in-situ stabilization of PCB impacted soil. - Addition of activated carbon to PCB impacted soil reduces equilibrium aqueous concentrations and uptake at the base of the terrestrial food chain.

Sistema energéticamente eficiente y de bajo costo para controlar la temperatura y aumentar el oxígeno en estanques de cultivo de alevines de tilapia roja

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Rubén Darío Vásquez-Salazar

2014-01-01

Full Text Available El artículo expone un sistema automático para controlar la temperatura y aumentar el oxígeno en estanques de cultivo de alevines de tilapia roja en la granja John Jairo González, del Politécnico Colombiano Jaime Isaza Cadavid. El control se realiza a través de un PLC que recibe dos señales de temperatura ubicadas en puntos diferentes del estanque, y una señal de un sensor de nivel. Ejecutando un algoritmo de control, activa o desactiva diferentes circuitos de agua fría, proveniente de la quebrada Guaracú, o caliente, a través de un proceso de bombeo de agua del estanque hacia los colectores solares, y regresándola al estanque, recirculación que se hace hasta obtener una temperatura de 29 °C, un poco por encima de la ideal de 28 °C. El sistema de control es validado comparando temperatura y oxígeno con un tanque sin control. 

Assessing the effects of FBC ash treatments of metal-contaminated soils using life history traits and metal bioaccumulation analysis of the earthworm Eisenia andrei

Energy Technology Data Exchange (ETDEWEB)

Grumiaux, F.; Demuynck, S.; Schikorski, D.; Lemiere, S.; Lepretre, A. [Universite Lille Nord de France, Villeneuve Dascq (France)

2010-03-15

Earthworms (Eisenia andrei) were exposed, in controlled conditions, to metal-contaminated soils previously treated in situ with two types of fluidized bed combustion ashes. Effects on this species were determined by life history traits analysis. Metal immobilizing efficiency of ashes was indicated by metal bioaccumulation. Ashes-treated soils reduced worm mortality compared to the untreated soil. However, these ashes reduced both cocoon hatching success and hatchlings numbers compared to the untreated soil. In addition, sulfo-calcical ashes reduced or delayed worm maturity and lowered cocoon production compared to silico-alumineous ones. Metal immobilizing efficiency of ashes was demonstrated for Zn, Cu and to a lesser extent Pb. Only silico-alumineous ashes reduced Cd bioaccumulation, although Cd was still bioconcentrated. Thus, although ash additions to metal-contaminated soils may help in immobilizing metals, their use might result, depending on the chemical nature of ashes, to severe detrimental effects on earthworm reproduction with possible long term consequences to populations.

Impact of heavy metal contamination on oxidative stress of Eisenia andrei and bacterial community structure in Tunisian mine soil.

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Boughattas, Iteb; Hattab, Sabrine; Boussetta, Hamadi; Banni, Mohamed; Navarro, Elisabeth

2017-08-01

The aims of this work were firstly to study the effect of heavy metal-polluted soils from Tunisian mine on earthworm biochemical biomarkers and on bacterial communities and therefore to analyze the interaction between earth worms and bacterial communities in these contaminated soils. For this purpose, we had introduced earthworm Eisenia andrei in six soils: one from mine spoils and five from agricultural soils, establishing a gradient of contamination. The response of worms to the presence of heavy metal was analyzed at the biochemical and transcriptional levels. In a second time, the impact of worm on bacterial community structure was investigated using automated ribosomal intergenic spacer analysis (ARISA) fingerprinting. An impact of heavy metal-contaminated soils on the oxidative status of E. andrei was observed, but this effect was dependent of the level of heavy metal contamination. Moreover, our results demonstrate that the introduction of earthworms E. andrei has an impact on bacterial community; however, the major change was observed in the less contaminated site. Furthermore, a significant correlation between earthworm oxidative status biomarkers and bacterial community structure was observed, mainly in the mine spoils. Therefore, we contribute to a better understanding of the relationships between epigenic earthworms and bacterial communities in heavy metal-contaminated soils.

Effects of metals on life cycle parameters of the earthworm Eisenia fetida exposed to field-contaminated, metal-polluted soils

International Nuclear Information System (INIS)

Nahmani, Johanne; Hodson, Mark E.; Black, Stuart

2007-01-01

Two control and eight field-contaminated, metal-polluted soils were inoculated with Eisenia fetida (Savigny, 1826). Three, 7, 14, 21, 28 and 42 days after inoculation, earthworm survival, body weight, cocoon production and hatching rate were measured. Seventeen metals were analysed in E. fetida tissue, bulk soil and soil solution. Soil organic carbon content, texture, pH and cation exchange capacity were also measured. Cocoon production and hatching rate were more sensitive to adverse conditions than survival or weight change. Soil properties other than metal concentration impacted toxicity. The most toxic soils were organic-poor (1-10 g C kg -1 ), sandy soils (c. 74% sand), with intermediate metal concentrations (e.g. 7150-13,100 mg Pb kg -1 , 2970-53,400 mg Zn kg -1 ). Significant relationships between soil properties and the life cycle parameters were determined. The best coefficients of correlation were generally found for texture, pH, Ag, Cd, Mg, Pb, Tl, and Zn both singularly and in multivariate regressions. Studies that use metal-amended artificial soils are not useful to predict toxicity of field multi-contaminated soils. - Soil pH, organic carbon content and texture can exert a greater influence on earthworm life cycle parameters than soil metal concentrations at metal-contaminated sites

Effects of metals on life cycle parameters of the earthworm Eisenia fetida exposed to field-contaminated, metal-polluted soils

Energy Technology Data Exchange (ETDEWEB)

Nahmani, Johanne [Department of Soil Science, School of Human and Environmental Sciences, University of Reading, Whiteknights, Reading, Berkshire RG6 6DW (United Kingdom)]. E-mail: nahmani@univ-metz.fr; Hodson, Mark E. [Department of Soil Science, School of Human and Environmental Sciences, University of Reading, Whiteknights, Reading, Berkshire RG6 6DW (United Kingdom)]. E-mail: m.e.hodson@reading.ac.uk; Black, Stuart [Department of Archaeology, School of Human and Environmental Sciences, Whiteknights, University of Reading, Reading RG6 6DW (United Kingdom)

2007-09-15

Two control and eight field-contaminated, metal-polluted soils were inoculated with Eisenia fetida (Savigny, 1826). Three, 7, 14, 21, 28 and 42 days after inoculation, earthworm survival, body weight, cocoon production and hatching rate were measured. Seventeen metals were analysed in E. fetida tissue, bulk soil and soil solution. Soil organic carbon content, texture, pH and cation exchange capacity were also measured. Cocoon production and hatching rate were more sensitive to adverse conditions than survival or weight change. Soil properties other than metal concentration impacted toxicity. The most toxic soils were organic-poor (1-10 g C kg{sup -1}), sandy soils (c. 74% sand), with intermediate metal concentrations (e.g. 7150-13,100 mg Pb kg{sup -1}, 2970-53,400 mg Zn kg{sup -1}). Significant relationships between soil properties and the life cycle parameters were determined. The best coefficients of correlation were generally found for texture, pH, Ag, Cd, Mg, Pb, Tl, and Zn both singularly and in multivariate regressions. Studies that use metal-amended artificial soils are not useful to predict toxicity of field multi-contaminated soils. - Soil pH, organic carbon content and texture can exert a greater influence on earthworm life cycle parameters than soil metal concentrations at metal-contaminated sites.

1H NMR-Based Metabolomic Analysis of Sub-Lethal Perfluorooctane Sulfonate Exposure to the Earthworm, Eisenia fetida, in Soil

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Myrna J. Simpson

2013-08-01

Full Text Available 1H NMR-based metabolomics was used to measure the response of Eisenia fetida earthworms after exposure to sub-lethal concentrations of perfluorooctane sulfonate (PFOS in soil. Earthworms were exposed to a range of PFOS concentrations (five, 10, 25, 50, 100 or 150 mg/kg for two, seven and fourteen days. Earthworm tissues were extracted and analyzed by 1H NMR. Multivariate statistical analysis of the metabolic response of E. fetida to PFOS exposure identified time-dependent responses that were comprised of two separate modes of action: a non-polar narcosis type mechanism after two days of exposure and increased fatty acid oxidation after seven and fourteen days of exposure. Univariate statistical analysis revealed that 2-hexyl-5-ethyl-3-furansulfonate (HEFS, betaine, leucine, arginine, glutamate, maltose and ATP are potential indicators of PFOS exposure, as the concentrations of these metabolites fluctuated significantly. Overall, NMR-based metabolomic analysis suggests elevated fatty acid oxidation, disruption in energy metabolism and biological membrane structure and a possible interruption of ATP synthesis. These conclusions obtained from analysis of the metabolic profile in response to sub-lethal PFOS exposure indicates that NMR-based metabolomics is an excellent discovery tool when the mode of action (MOA of contaminants is not clearly defined.

Lethal critical body residues as measures of Cd, Pb, and Zn bioavailability and toxicity in the earthworm Eisenia fetida

Energy Technology Data Exchange (ETDEWEB)

Conder, J.M.; Lanno, R.P. [Oklahoma State Univ., Dept. of Zoology, Stillwater, OK (United States)

2003-07-01

Background. Earthworm heavy metal concentrations (critical body residues, CBRs) may be the most relevant measures of heavy metal bioavailability in soils and may be linkable to toxic effects in order to better assess soil ecotoxicity. However, as earthworms possess physiological mechanisms to secrete and/or sequester absorbed metals as toxicologically inactive forms, total earthworm metal concentrations may not relate well with toxicity. Objective. The objectives of this research were to: i) develop LD{sub 50}s (total earthworm metal concentration associated with 50% mortality) for Cd, Pb, and Zn; ii) evaluate the LD{sub 50} for Zn in a lethal Zn-smelter soil; iii) evaluate the lethal mixture toxicity of Cd, Pb, and Zn using earthworm metal concentrations and the toxic unit (TU) approach; and iv) evaluate total and fractionated earthworm concentrations as indicators of sublethal exposure. Methods. Earthworms (Eisenia fetida (Savigny)) were exposed to artificial soils spiked with Cd, Pb, Zn, and a Cd-Pb-Zn equitoxic mixture to estimate lethal CBRs and mixture toxicity. To evaluate the CBR developed for Zn, earthworms were also exposed to Zn-contaminated field soils receiving three different remediation treatments. Earthworm metal concentrations were measured using a procedure devised to isolate toxicologically active metal burdens via separation into cytosolic and pellet fractions. (orig.)

Relating environmental availability to bioavailability: soil-type-dependent metal accumulation in the oligochaete Eisenia andrei.

Science.gov (United States)

Peijnenburg, W J; Baerselman, R; de Groot, A C; Jager, T; Posthuma, L; Van Veen, R P

1999-11-01

Body residues are often better estimates of the amount of a chemical at the sites of toxic action in an organism than ambient soil concentrations, because bioavailability differences among soils are explicitly taken into account in considerations of body residues. Often, however, insufficient attention is paid to the rate and extent at which tissue concentrations respond to soil concentrations and soil characteristics. In this contribution the impact of soil characteristics on the environmental bioavailability of heavy metals for the oligochaete worm Eisenia andrei is reported. Uptake of As, Cd, Cr, Cu, Ni, Pb, and Zn in 20 Dutch field soils and in OECD artificial soil was quantified as a function of time. Internal metal concentrations varied less than the corresponding external levels. Metal uptake and elimination were both metal- and species-dependent. Worms typically attained steady-state concentrations rapidly for Cr, Cu, Ni, and Zn. Internal concentrations similar to those in the cultivation medium, linearly increasing body concentrations, or steady-state internal concentrations well above those in the cultivation medium were found for As, Cd, and Pb. Multivariate expressions were derived to describe uptake rate constants, steady-state concentrations, and bioaccumulation factors as a function of soil characteristics. Soil acidity is the most important solid-phase characteristic modulating the availability of As, Cd, and Pb. Although additional semimechanistic calculations yielded evidence of pore-water-related uptake of Cd and Pb modulated by competition between H(+) and metal ions at the active sites of the membranes, the findings for Cr, Cu, Ni, and Zn point to additional influences, among which is probably regulation. Copyright 1999 Academic Press.

Indução de ivermectina na hormese sobre Eisenia foetida durante a vermicompostagem de esterco bovino

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Sandro M. Tuerlinckx

2015-07-01

Full Text Available Os medicamentos veterinários são, em sua maioria, excretados nas fezes de animais tratados demonstrando que os fármacos da classe das avermectinas podem ter efeitos nocivos sobre os organismos não alvo, caso se utilizem das fezes. O objetivo deste estudo foi determinar os efeitos subletais da ivermectina sobre as minhocas Eisenia foetida expostas às concentrações de ivermectina a 0, 1, 5, 10, 50 e 100 mg kg-1; amostras foram tomadas nos dias 7, 14 e 28 de exposição para determinação da sobrevivência, crescimento, produção de casulos e atividades enzimáticas, além de estudadas as alterações químicas e a atividade microbiana do vermicomposto não se observando, no entanto, efeitos negativos da ivermectina quanto à mortalidade de minhocas; da mesma forma, as características químicas e a atividade microbiana do vermicomposto não foram afetadas porém os resultados demonstraram que a ivermectina induz, quando em baixas concentrações, a um aumento na atividade da fosfatase alcalina e no crescimento de E. foetida mas altas concentrações inibiram esses parâmetros, o que foi refletido numa curva em forma de U invertido, uma representação gráfica do efeito hormético.

Vermicompostagem de lodo de curtume em associação com esterco bovino utilizando Eisenia fetida

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Guilherme Malafaia

2015-12-01

Full Text Available RESUMO O lodo gerado pela indústria curtumeira é um exemplo de resíduo que provoca graves problemas ambientais. Neste sentido, o objetivo do presente estudo foi avaliar a vermicompostagem de lodo de curtume, como método de reaproveitamento desse resíduo. Devido às características tóxicas do lodo de curtume, o mesmo foi misturado com esterco em várias proporções, em base seca. Durante 105 dias, a biomassa e a população das minhocas (Eisenia fetida foram avaliadas, além do pH, humidade e temperatura das unidades experimentais estabelecidas. Os substratos foram quimicamente caracterizados no início e no final do processo de vermicompostagem. Nenhuma diferença significativa foi observada em pH nos grupos com menores concentrações de lodo, no entanto, houve uma diminuição significativa no pH nos grupos com maiores concentrações do resíduo durante a vermicompostagem. As concentrações de Mg, Fe, Mn e Zn foram elevadas no vermicompostagem em todos os tratamentos. A relação C/N foi reduzida em todos os tratamentos, indicando o amadurecimento do substrato final. Os resultados indicam que vermicompostagem promove a conversão do lodo em composto em um curto intervalo de tempo, o que constitui um processo biotecnológico de tratamento capaz de adicionar o valor agronômico para este resíduo.

Effects of low-molecular-weight organic acids on the acute lethality, accumulation, and enzyme activity of cadmium in Eisenia fetida in a simulated soil solution.

Science.gov (United States)

Liu, Hai-Long; Wang, Yu-Jun; Xuan, Liang; Dang, Fei; Zhou, Dong-Mei

2017-04-01

In the present study, the effects of low-molecular-weight organic acids (LMWOAs) on the toxicity of cadmium (Cd) to Eisenia fetida were investigated in a simulated soil solution. The LMWOAs protected E. fetida from Cd toxicity, as indicated by the increased median lethal concentration (LC50) values and the increased activity of superoxide dismutase. In addition, Cd concentrations in E. fetida decreased dramatically in the presence of LMWOAs. These results were likely because of the complexation between Cd and LMWOAs, which decreased the bioavailability and consequential toxicity of Cd to E. fetida. Notably, LMWOAs reduced Cd toxicity in decreasing order (ethylenediamine tetraacetic acid [EDTA] > citric acid > oxalic acid > malic acid > acetic acid), which was consistent with the decreasing complexation constants between LMWOAs and Cd. These results advance our understanding of the interactions between Cd and LMWOAs in soil. Environ Toxicol Chem 2017;36:1005-1011. © 2016 SETAC. © 2016 SETAC.

BIOSORCIÓN DE Cd, Pb y Zn POR BIOMASA PRETRATADA DE ALGAS ROJAS, CÁSCARA DE NARANJA Y TUNA

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Lissette Vizcaíno Mendoza

2015-01-01

Full Text Available Debido a su movilidad en los ecosistemas y a su toxicidad para las formas superiores de vida,los metales pesados Cd, Pb y Zn son priorizados como unos de los contaminantes inorgánicosmás importantes debido al alto riesgo que representan para el medio ambiente. Con el objeto dedisminuir su concentración se diseñó un sistema para evaluar su remoción empleando biomasade algas rojas, cáscaras de naranja (Citrus sp. y tuna guajira (Opuntia sp.. Se estudió la influenciadel pretratamiento y el empaquetamiento mediante ensayos tipo batch, en los que se emplearonsoluciones de sodio y calcio. Se obtuvo como resultado una mayor capacidad de sorción de lasalgas modificadas con NaOH 0.1 N y de la naranja y la tuna con modificación sucesiva con NaOH yCaCl20.2 M, y una afección poco significativa (≤1% del proceso de sorción al empacar la biomasael placas planas de tul poliéster. La eficiencia de remoción se determinó mediante un reactor deflujo continuo de columna fija con un volumen líquido de 400 mL, 75 g de biomasa y tiemposde retención promedio de 1 y 2 h. Los resultados mostraron una eficiencia similar de las tresbiomasas para remover Cd y Pb, con promedios superiores al 95%, mientras que el Zn se removiócon mejor eficiencia (62% al emplear tuna modificada como sorbente. Finalmente, el materialse calcinó a 700 °C con lo que se obtuvo una ceniza estable frente a soluciones ácidas, lo cualgarantiza la captura de los metales removidos.

Assessment of soil stabilization by chemical extraction and bioaccumulation using earthworm, Eisenia fetida

Science.gov (United States)

Lee, Byung-Tae; Abd Aziz, Azilah; Han, Heop Jo; Kim, Kyoung-Woong

2014-05-01

Soil stabilization does not remove heavy metals from contaminated soil, but lowers their exposures to ecosystem. Thus, it should be evaluated by measuring the fractions of heavy metals which are mobile and/or bioavailable in soils. The study compared several chemical extractions which intended to quantify the mobile or bioaccessible fractions with uptake and bioaccumulation by earthworm, Eisenia fetida. Soil samples were taken from the abandoned mine area contaminated with As, Cd, Cu, Pb and/or Zn. To stabilize heavy metals, the soils were amended with limestone and steel slag at 5% and 2% (w/w), respectively. All chemical extractions and earthworm tests were applied to both the contaminated and the stabilized soils with triplicates. The chemical extractions consisted of six single extractions which were 0.01M CaCl2 (unbufferred), EDTA or DTPA (chelating), TCLP (acidic), Mehlich 3 (mixture), and aqua regia (peudo-total). Sequential extractions were also applied to fractionate heavy metals in soils. In earthworm tests, worms were exposed to the soils for uptake of heavy metals. After 28 days of exposure to soils, worms were transferred to clean soils for elimination. During the tests, three worms were randomly collected at proper sampling events. Worms were rinsed with DI water and placed on moist filter paper for 48 h for depuration. Filter paper was renewed at 24 h to prevent coprophagy. The worms were killed with liquid nitrogen, dried in the oven, and digested with aqua regia for ICP-MS analysis. In addition to the bioaccumulation, several toxicity endpoints were observed such as burrowing time, mortality, cocoon production, and body weight changes. Toxicokinetics was applied to determine the uptake and elimination heavy metals by the earthworms. Bioaccumulation factor (BAF) was estimated using total metal concentrations and body burdens. Pearson correlation and simple linear regression were applied to evaluate the relationship between metal fractions by single

Comparative toxicity in earthworms Eisenia fetida and Lumbricus terrestris exposed to cadmium nitrate using artificial soil and filter paper protocols

Energy Technology Data Exchange (ETDEWEB)

Fitzpatrick, L.C.; Goven, A.J. [Univ. of North Texas, Denton, TX (United States); Muratti-Ortiz, J.F. [City of Denton Water/Wastewater Laboratory, TX (United States); Venables, B.J. [TRAC Laboratories Inc., Denton, TX (United States)

1996-07-01

Earthworms are ideal soil organisms for use in terrestrial ecotoxicology. As such, several earthworm protocols have been developed for testing toxic potential of chemicals and contaminated soils. Of these, the 48-h filter paper contact (FP) and the 14-d artificial soil exposure (AS) protocols, using mortality (LC50) as the toxic endpoint and Eisenia fetida as the test species, have received the most attention, with the latter being adopted by both OECD and EEC in Europe and the Environmental Protection Agency (USEPA) in the United States. Although the FP technique, adopted by EEC, provides for inexpensive reproducible toxicity screening for chemicals (i.e. establishing relative toxicities), it has been criticized for lacking the ecotoxicological relevance of the AS protocol. Choice of earthworm species for laboratory testing also has been controversial. The manure worm, E. fetida, is criticized for not being sufficiently sensitive to chemicals or representative of {open_quotes}typical{close_quotes} earthworms. Lumbricus terrestris and Apporectodea caliginosa have been suggested as more sensitive and ecologically relevant earthworms by Dean-Ross and Martin, respectively. This paper compares the AS and FP protocols in assessing toxicity of cadminum to L. terrestris and E. fetida using LC50s and LC50s. 19 refs., 2 tabs.

Differences in p,p'-DDE bioaccumulation from compost and soil by the plants Cucurbita pepo and Cucurbita maxima and the earthworms Eisenia fetida and Lumbricus terrestris.

Science.gov (United States)

Peters, Richard; Kelsey, Jason W; White, Jason C

2007-07-01

Two plant species, Cucurbita pepo and Cucurbita maxima, and two earthworm species, Eisenia fetida and Lumbricus terrestris, were exposed to soil and compost with equivalent p,p'-DDE contamination. Pollutant bioconcentration was equal in plant roots in both media, but translocation was higher in C. pepo. Bioaccumulation by E. fetida was approximately 6- and 3-fold higher than that by L. terrestris in the soil and compost, respectively. For all species, p,p'-DDE uptake was significantly greater from soil than from compost; 7- to 8-fold higher for plant roots and 3- to 7-fold higher for worms. Abiotic desorption from soil was approximately twice that from the compost. When all the data are normalized for organic-carbon content of the media, the contaminant is more tightly bound by soil than compost. Although the risk associated with p,p'-DDE is higher in soil than compost, important mechanistic differences exist in contaminant binding to organic carbon in the two media.

Effects of Particle Size on Chemical Speciation and Bioavailability of Copper to Earthworms ( Eisenia fetida ) Exposed to Copper Nanoparticles

Energy Technology Data Exchange (ETDEWEB)

J Unrine; O Tsyusko; S Hunyadi; J Judy; P Bertsch

2011-12-31

To investigate the role of particle size on the oxidation, bioavailability, and adverse effects of manufactured Cu nanoparticles (NPs) in soils, we exposed the earthworm Eisenia fetida to a series of concentrations of commercially produced NPs labeled as 20- to 40-nm or <100-nm Cu in artificial soil media. Effects on growth, mortality, reproduction, and expression of a variety of genes associated with metal homeostasis, general stress, and oxidative stress were measured. We also used X-ray absorption spectroscopy and scanning X-ray fluorescence microscopy to characterize changes in chemical speciation and spatial distribution of the NPs in soil media and earthworm tissues. Exposure concentrations of Cu NPs up to 65 mg kg{sup -1} caused no adverse effects on ecologically relevant endpoints. Increases in metallothionein expression occurred at concentrations exceeding 20 mg kg-1 of Cu NPs and concentrations exceeding 10 mg kg{sup -1} of CuSO{sub 4} Based on the relationship of Cu tissue concentration to metallothionein expression level and the spatial distribution and chemical speciation of Cu in the tissues, we conclude that Cu ions and oxidized Cu NPs were taken up by the earthworms. This study suggests that oxidized Cu NPs may enter food chains from soil but that adverse effects in earthworms are likely to occur only at relatively high concentrations (>65 mg Cu kg{sup -1} soil).

Could humic acid relieve the biochemical toxicities and DNA damage caused by nickel and deltamethrin in earthworms (Eisenia foetida)?

Science.gov (United States)

Shen, Chen-Chao; Shen, Dong-Sheng; Shentu, Jia-Li; Wang, Mei-Zhen; Wan, Ming-Yang

2015-12-01

The aim of the study was to determine whether humic acid (HA) prevented gene and biochemical toxic effects in earthworms (Eisenia foetida) exposed to nickel and deltamethrin (at 100 and 1 mg kg(-1), respectively) in soil. Cellular- and molecular-level toxic effects of nickel and deltamethrin in earthworms were evaluated by measuring damage to lipid membranes and DNA and the production of protein carbonyls over 42 days of exposure. Nickel and deltamethrin induced significant levels of oxidative stress in earthworms, increasing the production of peroxidation products (malondialdehyde and protein carbonyls) and increasing the comet assay tail DNA% (determined by single-cell gel electrophoresis). DNA damage was the most sensitive of the three indices because it gave a higher sample/control ratio than did the other indices. The presence of HA alleviated (in decreasing order of effectiveness) damage to DNA, proteins, and lipid membranes caused by nickel and deltamethrin. A low HA dose (0.5-1% HA in soil) prevented a great deal of lipid membrane damage, but the highest HA dose (3% HA in soil) prevented still more DNA damage. However, the malondialdehyde concentrations in earthworms were higher at the highest HA dose than at the lower HA doses. The amounts of protein carbonyls produced at different HA doses were not significantly different. The toxic effects to earthworms caused by increased oxidizable nickel concentrations could be relieved by adding HA.

Effects of soil organic matter content on cadmium toxicity in Eisenia fetida: implications for the use of biomarkers and standard toxicity tests.

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Irizar, A; Rodríguez, M P; Izquierdo, A; Cancio, I; Marigómez, I; Soto, M

2015-01-01

Bioavailability is affected by soil physicochemical characteristics such as pH and organic matter (OM) content. In addition, OM constitutes the energy source of Eisenia fetida, a well established model species for soil toxicity assessment. The present work aimed at assessing the effects of changes in OM content on the toxicity of Cd in E. fetida through the measurement of neutral red uptake (NRU) and mortality, growth, and reproduction (Organisation for Economic Co-operation and Development [OECD] Nos. 207 and 222). Complementarily, metallothionein (MT) and catalase transcription levels were measured. To decrease variability inherent to natural soils, artificial soils (Organization for Economic Cooperation and Development 1984) with different OM content (6, 10, and 14%) and spiked with Cd solutions at increasing concentrations were used. Low OM in soil decreased soil ingestion and Cd bioaccumulation but also increased Cd toxicity causing lower NRU of coelomocytes, 100 % mortality, and stronger reproduction impairment, probably due to the lack of energy to maintain protection mechanisms (production of MT).Cd bioaccumulation did not reflect toxicity, and OM played a pivotal role in Cd toxicity. Thus, OM content should be taken into account when using E. fetida in in vivo exposures for soil health assessment.

Selección por conformación de reproductores de tilapia roja Oreochromis sp., mediante prueba de progenie

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Hermes Pineda S.

2013-10-01

Full Text Available Objetivo. Contribuir con la caracterización estadística y genotípica (media, media del error estándar, varianza y heredabilidad de la Longitud Total (LT y Longitud Altura del Lomo (LAL, y plantear una estrategia de selección para mejorar la calidad de la semilla de reproductores de tilapia roja Oreochromis sp. Materiales y métodos. El proyecto se realizó en la Granja Experimental y de Producción Piscícola (PCJIC, a un Km del municipio de San Jerónimo, distante 61 Km al Occidente de Medellín (Antioquia. Se conformaron tres grupos de cruzamientos, cada uno en proporción de 3 hembras por 1 macho así: un cruce Hembras Blancas (HB x Macho Rojo (MR y dos cruces Hembras Blancas (HB x Macho Blanco (MB. Las progenies fueron medidas a los 60 días post eclosión, teniendo en cuenta los supuestos estadísticos. Resultados. El ANOVA mostró que las progenies difieren significativamente (p<0.001 para ambas variables continuas. La LT fue mayor para la progenie del cruzamiento HBxMB sin reversión (8.04±1.04cm y LAL fue mayor para la progenie HBxMR (2.33±0.44cm. La correlación pareada entre las variables fue altamente significativa (r≥0.79, p<0.001. Las heredabilidades fueron 0.59 (LT y 0.64 (LAL. Los porcentajes de manchas en las progenies fueron HBxMR (34% y HBxMB no revertida (66%. Conclusiones. La progenie del Grupo 1 HBxMR tuvo los reproductores para una mejor selección por conformación, por lo que se sugiere aumentar el número de animales con mayor LAL. También, considerar la menor cantidad de manchas oscuras y realizar un manejo de reproductores por grupos independientes para selección masal.

Evaluación de la reacción de nueve genotipos de papa (Solanum tuberosumsubsp. andigena al ataque de Phytophthora infestans (Mont de Bary

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Betancourth G. Carlos

2008-12-01

Full Text Available

El presente trabajo se realizó en el municipio de Pasto, departamento de Nariño, Colombia, a 2.710 msnm. El objetivo fue evaluar la reacción de los clones de papa Blanca, Pintada, Rosada tipo Capiro, Roja Guata, Roja Redonda y Roja Loca y tres variedades mejoradas: Parda Suprema, Roja Nariño y Betina, al ataque de la gota (Phytophthora infestans. Se utilizó un diseño de bloques al azar con cuatro repeticiones y nueve tratamientos. Para la variable severidad los mejores genotipos fueron Blanca, Pintada, Parda Suprema y Betina con un promedio de 19,72; 21,99; 23,15 y 23,62%, respectivamente. El mejor rendimiento fue obtenido por los genotipos Blanca, Pintada, Parda Suprema, Betina y Roja Loca con 29,61; 24,66; 25,71; 27,12 y 28,05 t•ha^-1, en su orden.

A comparison of POPs bioaccumulation in Eisenia fetida in natural and artificial soils and the effects of aging

International Nuclear Information System (INIS)

Vlčková, Klára; Hofman, Jakub

2012-01-01

The close relationship between soil organic matter and the bioavailability of POPs in soils suggests the possibility of using it for the extrapolation between different soils. The aim of this study was to prove that TOC content is not a single factor affecting the bioavailability of POPs and that TOC based extrapolation might be incorrect, especially when comparing natural and artificial soils. Three natural soils with increasing TOC and three artificial soils with TOC comparable to these natural soils were spiked with phenanthrene, pyrene, lindane, p,p′-DDT, and PCB 153 and studied after 0, 14, 28, and 56 days. At each sampling point, total soil concentration and bioaccumulation in earthworms Eisenia fetida were measured. The results showed different behavior and bioavailability of POPs in natural and artificial soils and apparent effects of aging on these differences. Hence, direct TOC based extrapolation between various soils seems to be limited. - Highlights: ► Artificial and natural soils with the same TOC content were used in this study. ► BAF and total concentration of five POPs were measured during 56 days after spiking. ► Bioaccumulation was significantly lower in NS than in AS with the same TOC. ► Direct extrapolation according to TOC was possible for soils with levels >10%. ► Microbial degradation probably influences PAHs bioavailability. - Organic matter is an important factor in the bioavailability of POPs in soils, but direct extrapolation based on TOC content might lead to incorrect results in risk assessment.

Oxidative Stress, Cytotoxicity and Genotoxicity in Earthworm Eisenia fetida at Different Di-n-Butyl Phthalate Exposure Levels.

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Tingting Ma

Full Text Available Recognized as ubiquitous contaminants in soil, the environmental risk of phthalic acid esters (PAEs is of great concern recently. Effects of di-n-butyl phthalate (DnBP, an extensively used PAE compound to Eisenia fetida have been investigated in spiked natural brown yellow soil (Alfisol for soil contact test. The toxicity of DnBP to E. fetida on the activity of superoxide dismutase (SOD activity, peroxidase (POD, reactive oxygen species (ROS content, and the apoptosis of coelomocytes and DNA damage at the 7th, 14th, 21st and 28th day of the incubation have been paid close attention to. In general, SOD activity and ROS content were significantly induced, opposite to total protein content and POD activity, during the toxicity test of 28 days especially under concentrations higher than 2.5 mg kg-1. The reduction in neutral red retention (NRR time along with the increase of dead coelomocytes as the increasing of DnBP concentrations, indicating severe damage to cell viability under varying pollutant stress during cultivation, which could also be proved by comet assay results for exerting evident DNA damage in coelomocytes. DnBP in spiked natural soil could indeed cause damage to tissues, coelomocytes and the nucleus of E. fetida. The key point of the apparent change in different indices presented around 2.5 mg DnBP kg-1 soil, which could be recommended as the threshold of DnBP soil contamination, so that further investigation on threshold values to other soil animals or microorganisms could be discussed.

Biochemical and genetic toxicity of the ionic liquid 1-octyl-3-methylimidazolium chloride on earthworms (Eisenia fetida).

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Guo, Yingying; Liu, Tong; Zhang, Jun; Wang, Jinhua; Wang, Jun; Zhu, Lusheng; Yang, Jinhui

2016-02-01

Ionic liquids also known as "green solvents," are used in many fields. However, the dispersion of ionic liquids in soil systems is likely to cause damage to soil organisms. The objective of the present study was to investigate the toxicity of 1-octyl-3-methylimidazolium chloride ([C8 mim]Cl) on earthworms (Eisenia fetida). For this purpose, earthworms were exposed to different concentrations of [C8 mim]Cl (0 mg/kg, 5 mg/kg, 10 mg/kg, 20 mg/kg, and 40 mg/kg artificial soil) and sampled at 7 d, 14 d, 21 d, and 28 d. The results indicated that [C8 mim]Cl could cause an accumulation of reactive oxygen species (ROS) in earthworms, even at the lowest concentration (5 mg/kg). Compared with the controls, during the [C8 mim]Cl exposure period, the activities of superoxide dismutase (SOD) and catalase (CAT) decreased and then increased, whereas the activities of peroxidase (POD) and glutathione S-transferase (GST) increased. These changes in the activities of antioxidant enzymes and GST indicated that [C8 mim]Cl could induce oxidative damage in earthworms. The malondialdehyde content was increased by high levels of [C8 mim]Cl at 14 d and 28 d, indicating that [C8 mim]Cl could lead to lipid peroxidation in earthworms. In addition, the degree of DNA damage significantly increased with increasing [C8 mim]Cl concentrations and exposure time. The present study shows that [C8 mim]Cl caused biochemical and genetic toxicity in earthworms. © 2015 SETAC.

Nutrient changes and biodynamics of Eisenia fetida during vermicomposting of water lettuce (Pistia sp.) biomass: a noxious weed of aquatic system.

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Suthar, Surindra; Pandey, Bhawna; Gusain, Rita; Gaur, Rubia Zahid; Kumar, Kapil

2017-01-01

This paper reports the results of vermicomposting of water lettuce biomass (WL) spiked with cow dung at ratios of 20, 40, 60, and 80 % employing Eisenia fetida. A total of four treatments were established and changes in chemical properties of mixtures were observed. Vermicomposting caused a decrease in pH, TOC, volatile solids, and C/N ratio by 1.01-1.08-fold, 0.85-0.92-fold, 0.94-0.96-fold, 0.56-0.70-fold, respectively, but increase in EC, tot N, tot P, tot K, tot Ca, tot Zn, tot Fe, and tot Cu, by 1.19-1.42-fold, 1.33-1.68-fold, 1.38-1.69-fold, 1.13-1.24-fold, 1.04-1.11-fold, 1.16-1.37-fold, 1.05-1.113-fold, 1.10-1.27-fold, respectively. Overall, the treatment with 60-80 % of WL showed the maximum decomposition and mineralization rates. The earthworm showed the growth and reproduction rate in considerable ranges in all treatment setups but setups with 60-80 % WL proportion exhibited the optimum results. Results reveal that biomass of water lettuce can be utilized effectively for production of valuable manure through vermicomposting system.

Development of a standardized reproduction toxicity test with the earthworm species Eisenia fetida andrei using copper, pentachlorophenol and 2,4-dichloroaniline

Energy Technology Data Exchange (ETDEWEB)

van Gestel, C.A.; van Dis, W.A.; van Breemen, E.M.; Sparenburg, P.M. (National Institute of Public Health and Environmental Protection, BA Bilthoven (Netherland))

1989-12-01

This article describes a standardized test method for determining the effect of chemical substances on the reproduction of the earthworm Eisenia fetida andrei. It is based on the existing guidelines for acute toxicity testing with earthworms, and for reasons of standardization the same artificial soil substrate and earthworm species were chosen as prescribed by these guidelines. After being preconditioned for one week in untreated soil, earthworms are exposed to the chemical substances for 3 weeks. The number of cocoons produced is determined, and cocoons are incubated in untreated artificial soil for 5 weeks to assess hatchability. Results are presented from toxicity experiments with pentachlorophenol, copper, and 2,4-dichloroaniline. For these compounds no-effect levels (NEL) for cocoon production were 32, 60-120, and 56 mg.kg-1 dry soil, respectively. Hatching of cocoons was influenced by pentachlorophenol (NEL, 10 mg.kg-1), but not by copper and dichloroaniline. Following exposure, earthworms were incubated in clean soil again to study the possibility of recovery of cocoon production. For copper and dichloroaniline earthworms did recover cocoon production to a level as high as the control level or even higher; in case of pentachlorophenol, cocoon production was still reduced after 3 weeks in clean soil.

Perfluoroalkylsulfonic and carboxylic acids in earthworms (Eisenia fetida): Accumulation and effects results from spiked soils at PFAS concentrations bracketing environmental relevance.

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Karnjanapiboonwong, Adcharee; Deb, Sanjit K; Subbiah, Seenivasan; Wang, Degeng; Anderson, Todd A

2018-05-01

Effects of perfluorobutanesulfonic acid (PFBS), perfluorohexanesulfonic acid (PFHxS), perfluorononanoic acid (PFNA), and perfluoroheptanoic acid (PFHpA) on earthworms (Eisenia fetida) in soils contaminated with these compounds at 0.1, 1, 10, 1,000, and 100,000 μg kg -1 dry weight, covering concentration levels found in background, biosolid-amended, and facility-surrounding soils, were investigated. Earthworms were exposed to spiked soil for 21 days. Concentrations of these compounds in earthworms after 21-d exposure ranged from below detection to 127 mg kg -1 wet weight with the rank order of PFNA > PFHxS > PFHpA > PFBS; no mortality of earthworms was observed in all treatments including controls, except PFBS at 1,000 μg kg -1 and all PFASs at 100,000 μg kg -1 . The highest weight loss (29%) was observed for earthworms exposed to PFNA at 100,000 μg kg -1 , which was significantly different from all other treatments except PFHpA at 100,000 μg kg -1 . These results are expected to fill some data gaps in toxicity of PFASs in terrestrial environments and provide helpful information on the potential for trophic transport of PFASs from soil to higher organisms. Copyright © 2018 Elsevier Ltd. All rights reserved.

Primera identificación molecular del transgen de la proteína fluorescente roja (RFP en peces Cebra (Danio rerio transgénicos ornamentales introducidos en el Perú

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Carlos Scotto

2013-01-01

Full Text Available En el presente trabajo se identificó por primera vez peces Cebra transgénicos (Danio rerio fluorescentes de color rojo, naranja y rosado introducidos al territorio peruano de acuarios locales utilizando la técnica de PCR para amplificar el transgen RFP perteneciente a la anémona marina Discosoma spp. Se encontró una expresión génica diferencial del transgen de la proteína fluorescente roja (RFP que determinaría una gradiente de bioluminiscencia para cada color entre los peces OVM analizados. Se realizó un análisis de secuencias de las dos variantes de la RFP junto con las seis variantes de la GFP de proteínas fluorescentes existentes en el Genbank que podrían ayudar a identificar rápidamente si son nuevos genes o si son nuevas variantes de éstas proteínas fluorescentes y que podrían ser utilizadas en otros OVMs hidrobiológicos a futuro. De este modo, desarrollar y optimizar las medidas de bioseguridad mediante su oportuna detección a nivel genético molecular.

Bioremediation of the Soils Contaminated with Cadmium and Chromium, by the Earthworm Eisenia fetida

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Elham Aseman- Bashiz1

2014-07-01

Full Text Available One of the most important environmental problems in the world is the soils contamination by heavy metals in the industrial areas, and especially the contamination of the agricultural lands. The use of earthworms to bioremediate the soils results in reducing the pollutants concentration through a bioaccumulation mechanism on the contaminants in the earthworm's body. Hence, the present study aimed to prove the biological effectiveness of Eisenia fetida earthworms in bioremediation the soils contaminated with chromium and cadmium. Concentration of chromium and cadmium pollution in soil was determined to be 0.04 mg/g and 0.08 mg/g respectively. 30 worms were added to 500 g soil samples. Chromium and cadmium concentration in soil and in the body of worms was measured at two time periods of 21 and 42 days. To measure the concentration of chromium and cadmium we used ICP spectrometry. Software in usage was SPSS version 17. There was a significant correlation between the reduction of chromium and cadmium metals in the soils and the accumulation of chromium and cadmium metals in the worm’s body. A significant decline of chromium levels of the soil was observed in the days 21 and 42 during the study compared to initial amount of 0.1 mg/g. on the other hand chromium concentration of the soil decreased from 0.14 mg/g to 0.1 mg/g after 42 days. Comparison of mortality in two different time periods showed that by passing the time and by increase in soil chromium and cadmium concentrations the death toll of worms rises. The increased mortality of worms in the soil at a concentration of 0.08 mg/g of chromium, say that using the worms for bioremediation is not recommended at such concentration of chromium but using the worms for the removal of cadmium at concentrations of 0.04 mg/g and 0.08 mg/g in the soil is recommended.

Effects of different gamma exposure regimes on reproduction in the earthworm Eisenia fetida (Oligochaeta)

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Hertel-Aas, Turid, E-mail: turid.hertel-aas@umb.no [Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Aas (Norway); Brunborg, Gunnar, E-mail: Gunnar.Brunborg@fhi.no [Department of Chemical Toxicology, Norwegian Institute of Public Health, P.O. Box 4404 Nydalen, 0403 Oslo (Norway); Jaworska, Alicja, E-mail: Alicja.Jaworska@nrpa.no [Department of Emergency Preparedness and Environmental Radioactivity, Norwegian Radiation Protection Authority, P.O. Box 55, 1332, Osteraas (Norway); Salbu, Brit, E-mail: brit.salbu@umb.no [Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Aas (Norway); Oughton, Deborah Helen, E-mail: deborah.oughton@umb.no [Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Aas (Norway)

2011-12-15

Ecological risk assessment of ionising radiation requires knowledge about the responses of individuals and populations to chronic exposures, including situations when exposure levels change over time. The present study investigated processes such as recovery and the adaptive response with respect to reproduction endpoints in the earthworm Eisenia fetida exposed to {sup 60}Co {gamma}-radiation. Furthermore, a crossed experiment was performed to investigate the influence of F0 parental and F1 embryonic irradiation history on the response of irradiated or non-irradiated F1 offspring. Recovery: The sterility induced by sub-chronic exposure at 17 mGy/h (accumulated dose: 25 Gy) was temporary, and 8 weeks after irradiation the worms had regained their reproductive capacity (number of viable offspring produced per adult per week). Adaptive response: Adult worms were continuously exposed at a low priming dose rate of 0.14 mGy/h for 12 weeks (accumulated dose: 0.24 Gy), followed by 14 weeks exposure at a challenge dose rate of 11 mGy/h. The results suggest a lack of adaptive response, since there were no significant differences in the effects on reproduction capacity between the primed and the unprimed groups after challenge doses ranging from 7.6 to 27 Gy. Crossed experiment: The effects of exposure at 11 mGy/h for 21 weeks on growth, sexual maturation and reproduction of offspring, derived either from parent worms and cocoons both exposed at 11 mGy/h, or from non-irradiated parents and cocoons (total accumulated dose 44 and 38 Gy, respectively) were compared. There were no significant differences between the two exposed offspring groups for any of the endpoints. The reproduction capacity was very low for both groups compared to the controls, but the reproduction seemed to be maintained at the reduced level, which could indicate acclimatisation or stabilisation. Finally, parental and embryonic exposures at 11 mGy/h did not affect reproduction in the F1 offspring as adults.

Identification of a cytochrome P450 gene in the earthworm Eisenia fetida and its mRNA expression under enrofloxacin stress.

Science.gov (United States)

Li, Yinsheng; Zhao, Chun; Lu, Xiaoxu; Ai, Xiaojie; Qiu, Jiangping

2018-04-15

Cytochrome P450 (CYP450) enzymes are a family of hemoproteins primarily responsible for detoxification functions. Earthworms have been used as a bioindicator of soil pollution in numerous studies, but no CYP450 gene has so far been cloned. RT-PCR and RACE-PCR were employed to construct and sequence the CYP450 gene DNA from the extracted mRNA in the earthworm Eisenia fetida. The cloned gene (EW1) has an open reading frame of 477bp. The 3'-terminal region contained both the consensus and the signature sequences characteristic of CYP450. It was closely related to the CYP450 gene from the flatworm genus Opisthorchis felineus with 87% homology. The predicted structure of the putative protein was 97% homologous to human CYP450 family 27. This gene has been deposited in GenBank (accession no. KM881474). Earthworms (E. fetida) were then exposed to 1, 10, 100, and 500mgkg -1 enrofloxacin in soils to explore the mRNA expression by real time qPCR. The effect of enrofloxacin on mRNA expression levels of EW1 exhibited a marked hormesis pattern across the enrofloxacin dose range tested. This is believed to be the first reported CYP450 gene in earthworms, with reference value for molecular studies on detoxification processes in earthworms. Copyright © 2017 Elsevier Inc. All rights reserved.

Efecto de las nanopartículas industriales TiO 2 , SiO 2 y ZnO sobre la viabilidad celular y expresión génica en médula ósea roja de mus musculus

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Jacquelyne Zarria-Romero

Full Text Available RESUMEN Objetivos Evaluar el efecto de las nanopartículas de ZnO, TiO2 y SiO2 sobre la viabilidad celular y la expresión génica de las interleuquinas 7 y 3 y del factor estimulante de colonias de granulocito - macrófago (GM-CSF en Mus musculus. Materiales y métodos Se extrajo médula ósea roja de cinco roedores (Balb/c para el estudio de viabilidad celular mediante la prueba de MTT. Por otro lado, grupos cinco roedores fueron inoculados vía intraperitoneal con dosis de 0,5; 1; 2,5; 5 y 10 mg/kg de nanopartículas de ZnO y SiO2 y de 5; 10; 15; 20 y 25 mg/kg de nanopartículas de TiO2, 30 h después, se obtuvo el ARN a partir de la médula ósea roja para los análisis de expresión génica empleando las técnicas de PCR y RT-PCR cuantitativa. Resultados Las nanopartículas de ZnO y SiO2 redujeron la viabilidad celular de una manera dosis-dependiente en un 37 y 26%, respectivamente, a partir de una dosis de 1 mg/kg. En cuanto al efecto sobre la expresión génica, a las dosis 5 y 10 mg/kg, las nanopartículas de TiO2 redujeron en mayor porcentaje la expresión de las interleuquinas 7 y 3 (55,3 y 70,2% respectivamente, con respecto a la expresión del GM-CSF, el mayor porcentaje de reducción lo produjo las nanopartículas de SiO2 (91%. Las nanopartículas de ZnO redujeron a partir de las dosis de 20 y 25 mg/kg. Conclusiones Las nanopartículas de ZnO, SiO2 y TiO2 alteran la viabilidad celular y la expresión génica en la médula ósea de ratón.

Influencia de la adición de un 2% de carbonato de calcio en el proceso de fabricación de los ladrillos de cerámica roja: etapas de secado y cocción

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Dania Betancourt

2013-08-01

Full Text Available El trabajo hace un estudio de la influencia de la adición del 2% de carbonato de calcio en la duración de la etapa de secado y la eficiencia de la etapa de cocción durante el proceso de fabricación de ladrillos de cerámica roja. Dicho estudio se realizó en una fábrica que produce ladrillos huecos con una capacidad instalada de 65000 ladrillos por cada quema .Los resultados del estudio realizado demostraron que la adición de un 2% de CaCO3 a la pasta arcillosa disminuye el tiempo de secado en un 35% con respecto a los ladrillos elaborados sin adición y provoca una disminución del consumo de combustible del 27 % con respecto a quemas anteriores realizadas sin la adición de este fundente debido a la reducción del tiempo de cocción en los hornos.

Influencia de la finura de molido del carbonato de calcio en las propiedades físico mecánicas y de durabilidad de los ladrillos de cerámica roja Influence of grinding fineness of calcium carbonate on physic-chemical properties and durability on red ceramic bricks

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Yosvany Díaz

2011-12-01

Full Text Available El trabajo hace un estudio de la influencia de la finura de molido del carbonato de calcio en las propiedades de Resistencia a la compresión, densidad, absorción y durabilidad de los ladrillos de cerámica roja, a través de la realización de ensayos físico- mecánicos y de envejecimiento acelerado, cuando este se adiciona por debajo del 10% en relación con el peso de la arcilla. Se demuestra que cuando dicho aditivo se muele hasta finuras entre las 72 m y 150 1m, no se afectan las propiedades mencionadas anteriormente, sin embargo no ocurre así cuando es molido hasta un tamaño de partícula de 297 1m. El estudio realizado, demuestra que con esta superficie específica, la adición de calcita afecta tanto las propiedades físico-mecánicas como de durabilidad de los ladrillos de cerámica roja.The paper exposes the results of a study, at macro structural level, on the influence of the calcite grinding process fineness on the red ceramic bricks following properties: Compressive strenggth, Density, Absorption and Durability, though the performance of physical - mechanical and accelerated aging tests, when this is added in an amount inferior to the 10% in relation with the clay weight. It is proved that when this admixture is ground until fineness between 72 im and 150 [im, the above mentioned properties are not affected, however it doesn't occur this way when it is ground until a particle size of 29m. The carried out study, demonstrates that with this specific surface, the calcite addition affects the red ceramic bricks physical-mechanical properties as well as their durability

Por qué soy “cronista rojo”

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Henry Holguín

2010-06-01

Full Text Available Refiere que la crónica roja ha sido maltratada, estigmatizada y prohibida desde hace años, más por motivos estéticos que éticos. No existe una técnica periodística para la crónica roja, porque no se enseña a tratarla en las universidades. La crónica roja necesariamente aparecerá mientras haya violencia, delincuencia, no se puede tener una doble moral, acota.

EVALUACIÓN DE ALTERNATIVAS PARA CLIMATIZACIÓN DE ESTANQUES CON ENERGÍA SOLAR PARA CULTIVO DE TILAPIA ROJA (Oreochromis sp), LOCALIZADOS EN LA ZONA FRÍA DEL VALLE DEL CAUCA, COLOMBIA EVALUATION OF ALTERNATIVES FOR AIR CONDITIONING OF PONDS WITH SOLAR ENERGY FOR CULTIVATION OF RED TILAPIA (Oreochromis sp), LOCATED IN THE COLD AREA OF THE CAUCA VALLEY, COLOMBIA

OpenAIRE

Francisco Javier Borja Gallardo; Luis Octavio González Salcedo; Victoria Eugenia Quintero de Vallejo

2006-01-01

La investigación fue desarrollada para que la tilapia roja (Oreochromis sp), especie de pez mÁs producida en Colombia, pueda ser explotada en lugares donde las condiciones óptimas para su normal desarrollo no son las adecuadas, como son las regiones altas con temperaturas por debajo de los 24 °C, aplicando metodologías relacionadas con el aprovechamiento de la energía solar. Las técnicas evaluadas fueron: climatización por medio de colectores solares, climatización por medio de manta térmica,...

Exposure to extremely low frequency (50 Hz electromagnetic field changes the survival rate and morphometric characteristics of neurosecretory neurons of the earthworm Eisenia foetida (Oligochaeta under illumination stress

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Banovački Zorana

2013-01-01

Full Text Available An in vivo model was set up to establish the behavioral stress response (rate of survival and morphometric characteristics of A1 protocerebral neurosecretory neurons (cell size of Eisenia foetida (Oligochaeta as a result of the synergetic effect of extremely low frequency electromagnetic fields (ELF-EMF - 50 Hz, 50 μT, 17 V/m and 50 Hz, 150 μT, 17 V/m, respectively and constant illumination (420-450 lux. If combined, these two stressors significantly (p<0.05 increased the survival rate of E. foetida in the 150 μT-exposed animals, because of delayed caudal autotomy reflex, an indicator of stress response. In addition, morphometric analysis indicated that there were changes in the protocerebral neurosecretory cells after exposure to the ELF-EMF. The present data support the view that short-term ELF-EMF exposure in “windows” of intensity is likely to stimulate the immune and neuroendocrine response of E. foetida.

Bioaccumulation of polycyclic aromatic hydrocarbons and survival of earthworms (Eisenia andrei) exposed to biochar amended soils.

Science.gov (United States)

Malev, O; Contin, M; Licen, S; Barbieri, P; De Nobili, M

2016-02-01

Biochar has a charcoal polycyclic aromatic structure which allows its long half-life in soil, making it an ideal tool for C sequestration and for adsorption of organic pollutants, but at the same time raises concerns about possible adverse impacts on soil biota. Two biochars were tested under laboratory-controlled conditions on Eisenia andrei earthworms: a biochar produced at low temperature from wine tree cuttings (WTB) and a commercial low tar hardwood lump charcoal (HLB). The avoidance test (48-h exposure) showed that earthworms avoid biochar-treated soil with rates higher than 16 t ha(-1) for HLB and 64 t ha(-1) for WTB. After 42 days, toxic effects on earthworms were observed even at application rates (100 t ha(-1)) that are generally considered beneficial for most crops. The concentration of HLB and WTB required to kill half of earthworms' population (LC50; 95% confidence limits) in the synthetic OECD soil was 338 and 580 t ha(-1), respectively. Accumulation of polycyclic aromatic hydrocarbons (PAH) in earthworms exposed to the two biochar types at 100 t ha(-1) was tested in two soils of different texture. In biochar-treated soils, the average earthworm survival rates were about 64% in the sandy and 78% clay-loam soils. PAH accumulation was larger in the sandy soil and largest in soils amended with HLB. PAH with less than four rings were preferentially scavenged from the soil by biochars, and this behaviour may mask that of the more dangerous components (i.e. four to five rings), which are preferentially accumulated. Earthworms can accumulate PAH as a consequence of exposure to biochar-treated soils and transfer them along the food chain. Soil type and biochar quality are both relevant in determining PAH transfer.

The existence of fertile hybrids of closely related model earthworm species, Eisenia andrei and E. fetida.

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Barbara Plytycz

Full Text Available Lumbricid earthworms Eisenia andrei (Ea and E. fetida (Ef are simultaneous hermaphrodites with reciprocal insemination capable of self-fertilization while the existence of hybridization of these two species was still debatable. During the present investigation fertile hybrids of Ea and Ef were detected. Virgin specimens of Ea and Ef were laboratory crossed (Ea+Ef and their progeny was doubly identified. 1 -identified by species-specific maternally derived haploid mitochondrial DNA sequences of the COI gene being either 'a' for worms hatched from Ea ova or 'f' for worms hatched from Ef ova. 2 -identified by the diploid maternal/paternal nuclear DNA sequences of 28s rRNA gene being either 'AA' for Ea, 'FF' for Ef, or AF/FA for their hybrids derived either from the 'aA' or 'fF' ova, respectively. Among offspring of Ea+Ef pairs in F1 generation there were mainly aAA and fFF earthworms resulted from the facilitated self-fertilization and some aAF hybrids from aA ova but none fFA hybrids from fF ova. In F2 generation resulting from aAF hybrids mated with aAA a new generations of aAA and aAF hybrids were noticed, while aAF hybrids mated with fFF gave fFF and both aAF and fFA hybrids. Hybrids intercrossed together produced plenty of cocoons but no hatchlings independently whether aAF+aAF or aAF+fFA were mated. These results indicated that Ea and Ef species, easy to maintain in laboratory and commonly used as convenient models in biomedicine and ecotoxicology, may also serve in studies on molecular basis of interspecific barriers and mechanisms of introgression and speciation. Hypothetically, their asymmetrical hybridization can be modified by some external factors.

Effects of earthworm (Eisenia fetida) and wheat (Triticum aestivum) straw additions on selected properties of petroleum-contaminated soils.

Science.gov (United States)

Callaham, Mac A; Stewart, Arthur J; Alarcón, Clara; McMillen, Sara J

2002-08-01

Current bioremediation techniques for petroleum-contaminated soils are designed to remove contaminants as quickly and efficiently as possible, but not necessarily with postremediation soil biological quality as a primary objective. To test a simple postbioremediation technique, we added earthworms (Eisenia fetida) or wheat (Triticum aestivum) straw to petroleum land-farm soil and measured biological quality of the soil as responses in plant growth, soil respiration, and oil and grease (O&G) and total petroleum hydrocarbon (TPH) concentrations. Results indicated that plant growth was greater in earthworm-treated land-farm soil. Furthermore, addition of wheat straw resulted in greater total respiration in all soils tested (land-farm soil, noncontaminated reference soil, and a 1:1 mixture of land-farm and reference soils). We observed a 30% increase in soil respiration in straw-amended oily soil, whereas respiration increased by 246% in straw-amended reference soil. Much of the difference between oily and reference soils was attributable to higher basal respiration rates of nonamended oily soil compared to nonamended reference soil. Addition of earthworms resulted in greater total respiration of all soil and straw treatments except two (the land-farm and the 1:1 mixture soil treatments without straw). Straw and earthworm treatments did not affect O&G or TPH concentrations. Nevertheless, our findings that earthworm additions improved plant growth and that straw additions enhanced microbial activity in land-farm soil suggest that these treatments may be compatible with plant-based remediation techniques currently under evaluation in field trials, and could reduce the time required to restore soil ecosystem function.

Evaluation of DNA damage and antioxidant system induced by di-n-butyl phthalates exposure in earthworms (Eisenia fetida).

Science.gov (United States)

Du, Li; Li, Guangde; Liu, Mingming; Li, Yanqiang; Yin, Suzhen; Zhao, Jie; Zhang, Xinyi

2015-05-01

Di-n-butyl phthalates (DBP) are recognized as ubiquitous contaminants in soil and adversely impact the health of organisms. The effect of DBP on the activity of antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT), malondialdehyde (MDA) content and DNA damage were used as biomarkers to analyze the relationship between DNA damage and oxidative stress and to evaluate the genotoxic effect of DBP on earthworms (Eisenia fetida). DBP was added to artificial soil in the amounts of 0, 5, 10, 50 and 100mg per kg of soil. Earthworm tissues exposed to each treatment were collected on the 7th, 14th, 21st, and 28th day of the treatment. The results showed that SOD and CAT levels were significantly inhibited in the 100mgkg(-1) treatment group on day 28. MDA content in treatment groups was higher than in the control group throughout the exposure time, suggesting that DBP may lead to oxidative stress in cells. A dose-response relationship existed between DNA damage and total soil DBP levels. The comet assay showed that increasing concentrations of DBP resulted in a gradual increase in the OTM, Comet Tail Length and Tail DNA %. The degree of DNA damage was increased with increasing concentration of DBP. These results suggested that DBP induced serious oxidative damage on earthworms and induced the formation of reactive oxygen species (ROS) in earthworms. The excessive generation of ROS caused damage to vital macromolecules including lipids and DNA. DBP in the soils were responsible for the exerting genotoxic effects on earthworms. Copyright © 2015 Elsevier Inc. All rights reserved.

1H NMR-based metabolomics of time-dependent responses of Eisenia fetida to sub-lethal phenanthrene exposure

International Nuclear Information System (INIS)

Lankadurai, Brian P.; Wolfe, David M.; Simpson, Andre J.; Simpson, Myrna J.

2011-01-01

1 H NMR-based metabolomics was used to examine the response of the earthworm Eisenia fetida after exposure to sub-lethal concentrations of phenanthrene over time. Earthworms were exposed to 0.025 mg/cm 2 of phenanthrene (1/64th of the LC 50 ) via contact tests over four days. Earthworm tissues were extracted using a mixture of chloroform, methanol and water, resulting in polar and non-polar fractions that were analyzed by 1 H NMR after one, two, three and four days. NMR-based metabolomic analyses revealed heightened E. fetida responses with longer phenanthrene exposure times. Amino acids alanine and glutamate, the sugar maltose, the lipids cholesterol and phosphatidylcholine emerged as potential indicators of phenanthrene exposure. The conversion of succinate to fumarate in the Krebs cycle was also interrupted by phenanthrene. Therefore, this study shows that NMR-based metabolomics is a powerful tool for elucidating time-dependent relationships in addition to the mode of toxicity of phenanthrene in earthworm exposure studies. - Highlights: → NMR-based earthworm metabolomic analysis of the mode of action of phenanthrene is presented. → The earthworm species E. fetida were exposed to sub-lethal phenanthrene concentrations. → Both polar and non-polar metabolites of E. fetida tissue extracts were analyzed by 1 H NMR. → Longer phenanthrene exposure times resulted in heightened earthworm responses. → An interruption of the Krebs cycle was also observed due to phenanthrene exposure. - 1 H NMR metabolomics is used to determine the relationship between phenanthrene exposure and the metabolic response of the earthworm E. fetida over time and also to elucidate the phenanthrene mode of toxicity.

Nickel in Soil Modifies Sensitivity to Diazinon Measured by the Activity of Acetylcholinesterase, Catalase, and Glutathione S-Transferase in Earthworm Eisenia fetida

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Agnieszka Zawisza-Raszka

2013-01-01

Full Text Available Nickel in typical soils is present in a very low concentration, but in the contaminated soils it occurs in locally elevated concentrations. The aim of this study was to examine the effect of nickel in the concentrations of 300 (very high, close to LOEC for reproduction and 900 (extremely high, close to LOEC for mortality mg/kg dry soil on the life history and acetylcholinesterase, catalase, and glutathione S-transferase activities in earthworm Eisenia fetida and to establish how nickel modifies the sensitivity to organophosphorous pesticide—diazinon. Cocoons production and juveniles’ number were significantly lower only in groups exposed to Ni in the concentration of 900 mg/kg dry soil for two months. Diazinon administration diminished the AChE activity in the GI tract and in the body wall. The interaction between diazinon and nickel was observed, and, in consequence, the AChE activity after the pesticide treatment was similar to controls in worms preexposed to nickel. Both pesticide administration and exposure to nickel caused an increase in the GST activity in examined organs and CAT activity in body wall. Both biometric and development data and simple enzymatic analysis, especially the AChE and GST, show a Ni pretreatment effect on the subsequent susceptibility to pesticide.

Differences in p,p'-DDE bioaccumulation from compost and soil by the plants Cucurbita pepo and Cucurbita maxima and the earthworms Eisenia fetida and Lumbricus terrestris

International Nuclear Information System (INIS)

Peters, Richard; Kelsey, Jason W.; White, Jason C.

2007-01-01

Two plant species, Cucurbita pepo and Cucurbita maxima, and two earthworm species, Eisenia fetida and Lumbricus terrestris, were exposed to soil and compost with equivalent p,p'-DDE contamination. Pollutant bioconcentration was equal in plant roots in both media, but translocation was higher in C. pepo. Bioaccumulation by E. fetida was approximately 6- and 3-fold higher than that by L. terrestris in the soil and compost, respectively. For all species, p,p'-DDE uptake was significantly greater from soil than from compost; 7- to 8-fold higher for plant roots and 3- to 7-fold higher for worms. Abiotic desorption from soil was approximately twice that from the compost. When all the data are normalized for organic-carbon content of the media, the contaminant is more tightly bound by soil than compost. Although the risk associated with p,p'-DDE is higher in soil than compost, important mechanistic differences exist in contaminant binding to organic carbon in the two media. - Availability of p,p'-DDE to earthworms and plants was dramatically different in soil and compost

Gene expression analysis of 4 biomarker candidates in Eisenia fetida exposed to an environmental metallic trace elements gradient: A microcosm study

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Brulle, Franck; Lemiere, Sebastien [Univ Lille Nord de France, F-59000 Lille (France); LGCgE, Equipe Ecologie Numerique et Ecotoxicologie, Lille 1, F-59650 Villeneuve d' Ascq (France); Waterlot, Christophe; Douay, Francis [Univ Lille Nord de France, F-59000 Lille (France); LGCgE, Equipe Sols et Environnement, Groupe ISA, 48 boulevard Vauban, F-59046 Lille Cedex (France); Vandenbulcke, Franck, E-mail: franck.vandenbulcke@univ-lille1.fr [Univ Lille Nord de France, F-59000 Lille (France); LGCgE, Equipe Ecologie Numerique et Ecotoxicologie, Lille 1, F-59650 Villeneuve d' Ascq (France)

2011-11-15

Past activities of 2 smelters (Metaleurop Nord and Nyrstar) led to the accumulation of high amounts of Metal Trace Elements (TEs) in top soils of the Noyelles-Godault/Auby area, Northern France. Earthworms were exposed to polluted soils collected in this area to study and better understand the physiological changes, the mechanisms of acclimation, and detoxification resulting from TE exposure. Previously we have cloned and transcriptionally characterized potential biomarkers from immune cells of the ecotoxicologically important earthworm species Eisenia fetida exposed in vivo to TE-spiked standard soils. In the present study, analysis of expression kinetics of four candidate indicator genes (Cadmium-metallothionein, coactosin like protein, phytochelatin synthase and lysenin) was performed in E. fetida after microcosm exposures to natural soils exhibiting an environmental cadmium (Cd) gradient in a kinetic manner. TE body burdens were also measured. This microcosm study provided insights into: (1) the ability of the 4 tested genes to serve as expression biomarkers, (2) detoxification processes through the expression analysis of selected genes, and (3) influence of land uses on the response of potential biomarkers (gene expression or TE uptake). - Highlights: {yields} Expression biomarkers in animals exposed to Cadmium-contaminated field soils. {yields} Expression kinetics to test the ability of genes to serve as expression biomarkers. {yields} Study of detoxification processes through the expression analysis of selected genes.

Design, validation and annotation of transcriptome-wide oligonucleotide probes for the oligochaete annelid Eisenia fetida.

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Ping Gong

Full Text Available High density oligonucleotide probe arrays have increasingly become an important tool in genomics studies. In organisms with incomplete genome sequence, one strategy for oligo probe design is to reduce the number of unique probes that target every non-redundant transcript through bioinformatic analysis and experimental testing. Here we adopted this strategy in making oligo probes for the earthworm Eisenia fetida, a species for which we have sequenced transcriptome-scale expressed sequence tags (ESTs. Our objectives were to identify unique transcripts as targets, to select an optimal and non-redundant oligo probe for each of these target ESTs, and to annotate the selected target sequences. We developed a streamlined and easy-to-follow approach to the design, validation and annotation of species-specific array probes. Four 244K-formatted oligo arrays were designed using eArray and were hybridized to a pooled E. fetida cRNA sample. We identified 63,541 probes with unsaturated signal intensities consistently above the background level. Target transcripts of these probes were annotated using several sequence alignment algorithms. Significant hits were obtained for 37,439 (59% probed targets. We validated and made publicly available 63.5K oligo probes so the earthworm research community can use them to pursue ecological, toxicological, and other functional genomics questions. Our approach is efficient, cost-effective and robust because it (1 does not require a major genomics core facility; (2 allows new probes to be easily added and old probes modified or eliminated when new sequence information becomes available, (3 is not bioinformatics-intensive upfront but does provide opportunities for more in-depth annotation of biological functions for target genes; and (4 if desired, EST orthologs to the UniGene clusters of a reference genome can be identified and selected in order to improve the target gene specificity of designed probes. This approach is

Evaluation of genotoxicity of the acute gamma radiation on earthworm Eisenia fetida using single cell gel electrophoresis technique (Comet assay).

Science.gov (United States)

Sowmithra, K; Shetty, N J; Jha, S K; Chaubey, R C

2015-12-01

Earthworms (Eisenia fetida) most suitable biological indicators of radioactive pollution. Radiation-induced lesions in DNA can be considered to be molecular markers for early effects of ionizing radiation. Gamma radiation produces a wide spectrum of DNA. Some of these lesions, i.e., DNA strand breaks and alkali labile sites can be detected by the single-cell gel electrophoresis (SCGE) or comet assay by measuring the migration of DNA from immobilized nuclear DNA. E. fetida were exposed to different doses of gamma radiation, i.e., 1, 5, 10, 20, 30, 40 and 50Gy, and comet assay was performed for all the doses along with control at 1, 3 and 5h post irradiation to evaluate the genotoxicity of gamma radiation in this organism. The DNA damage was measured as percentage of comet tail DNA. A significant increase in DNA damage was observed in samples exposed to 5Gy and above, and the increase in DNA damage was dose dependent i.e., DNA damage was increased with increased doses of radiation. The highest DNA damage was noticed at 1h post irradiation and gradually decreased with time, i.e., at 3 and 5h post irradiation. The present study reveals that gamma radiation induces DNA damage in E. fetida and the comet assay is a sensitive and rapid method for its detection to detect genotoxicity of gamma radiation. Copyright © 2015 Elsevier B.V. All rights reserved.

Differences in p,p'-DDE bioaccumulation from compost and soil by the plants Cucurbita pepo and Cucurbita maxima and the earthworms Eisenia fetida and Lumbricus terrestris

Energy Technology Data Exchange (ETDEWEB)

Peters, Richard [Program in Environmental Science and Department of Chemistry, Muhlenberg College, 2400 Chew Street, Allentown, PA 18104 (United States)]. E-mail: rp232604@muhlenberg.edu; Kelsey, Jason W. [Program in Environmental Science and Department of Chemistry, Muhlenberg College, 2400 Chew Street, Allentown, PA 18104 (United States)]. E-mail: kelsey@muhlenberg.edu; White, Jason C. [Department of Soil and Water, The Connecticut Agricultural Experiment Station, 123 Huntington Street, New Haven, CT 06504 (United States)]. E-mail: jason.white@po.state.ct.us

2007-07-15

Two plant species, Cucurbita pepo and Cucurbita maxima, and two earthworm species, Eisenia fetida and Lumbricus terrestris, were exposed to soil and compost with equivalent p,p'-DDE contamination. Pollutant bioconcentration was equal in plant roots in both media, but translocation was higher in C. pepo. Bioaccumulation by E. fetida was approximately 6- and 3-fold higher than that by L. terrestris in the soil and compost, respectively. For all species, p,p'-DDE uptake was significantly greater from soil than from compost; 7- to 8-fold higher for plant roots and 3- to 7-fold higher for worms. Abiotic desorption from soil was approximately twice that from the compost. When all the data are normalized for organic-carbon content of the media, the contaminant is more tightly bound by soil than compost. Although the risk associated with p,p'-DDE is higher in soil than compost, important mechanistic differences exist in contaminant binding to organic carbon in the two media. - Availability of p,p'-DDE to earthworms and plants was dramatically different in soil and compost.

Effects of biochar and the earthworm Eisenia fetida on the bioavailability of polycyclic aromatic hydrocarbons and potentially toxic elements

International Nuclear Information System (INIS)

Gomez-Eyles, Jose L.; Sizmur, Tom; Collins, Chris D.; Hodson, Mark E.

2011-01-01

Polycyclic aromatic hydrocarbons (PAHs) and potentially toxic elements (PTEs) were monitored over 56 days in calcareous contaminated-soil amended with either or both biochar and Eisenia fetida. Biochar reduced total (449 to 306 mg kg -1 ) and bioavailable (cyclodextrin extractable) (276 to 182 mg kg -1 ) PAHs, PAH concentrations in E. fetida (up to 45%) but also earthworm weight. Earthworms increased PAH bioavailability by >40%. Combined treatment results were similar to the biochar-only treatment. Earthworms increased water soluble Co (3.4 to 29.2 mg kg -1 ), Cu (60.0 to 120.1 mg kg -1 ) and Ni (31.7 to 83.0 mg kg -1 ) but not As, Cd, Pb or Zn; biochar reduced water soluble Cu (60 to 37 mg kg -1 ). Combined treatment results were similar to the biochar-only treatment but gave a greater reduction in As and Cd mobility. Biochar has contaminated land remediation potential, but its long-term impact on contaminants and soil biota needs to be assessed. - Research highlights: → Biochar reduced total and bioavailable PAH concentrations. → Biochar was less effective at immobilising PTEs, due to its low cation exchange capacity. → E. fetida increased PAH bioavailability and PTE mobility. → When used in combination biochar reduced the concentration of PTEs mobilised by E. fetida. → Biochar had a negative effect on E. fetida in terms of weight loss. - Biochar decreased PAH biovailability but was less effective at reducing PTE mobility, whilst E. fetida increased both PAH and PTE bioavailability.

Effects of biochar and the earthworm Eisenia fetida on the bioavailability of polycyclic aromatic hydrocarbons and potentially toxic elements

Energy Technology Data Exchange (ETDEWEB)

Gomez-Eyles, Jose L., E-mail: j.l.gomezeyles@reading.ac.u [University of Reading, School of Human and Environmental Sciences, Soil Research Centre, Reading, RG6 6DW Berkshire (United Kingdom); Sizmur, Tom; Collins, Chris D.; Hodson, Mark E. [University of Reading, School of Human and Environmental Sciences, Soil Research Centre, Reading, RG6 6DW Berkshire (United Kingdom)

2011-02-15

Polycyclic aromatic hydrocarbons (PAHs) and potentially toxic elements (PTEs) were monitored over 56 days in calcareous contaminated-soil amended with either or both biochar and Eisenia fetida. Biochar reduced total (449 to 306 mg kg{sup -1}) and bioavailable (cyclodextrin extractable) (276 to 182 mg kg{sup -1}) PAHs, PAH concentrations in E. fetida (up to 45%) but also earthworm weight. Earthworms increased PAH bioavailability by >40%. Combined treatment results were similar to the biochar-only treatment. Earthworms increased water soluble Co (3.4 to 29.2 mg kg{sup -1}), Cu (60.0 to 120.1 mg kg{sup -1}) and Ni (31.7 to 83.0 mg kg{sup -1}) but not As, Cd, Pb or Zn; biochar reduced water soluble Cu (60 to 37 mg kg{sup -1}). Combined treatment results were similar to the biochar-only treatment but gave a greater reduction in As and Cd mobility. Biochar has contaminated land remediation potential, but its long-term impact on contaminants and soil biota needs to be assessed. - Research highlights: Biochar reduced total and bioavailable PAH concentrations. Biochar was less effective at immobilising PTEs, due to its low cation exchange capacity. E. fetida increased PAH bioavailability and PTE mobility. When used in combination biochar reduced the concentration of PTEs mobilised by E. fetida. Biochar had a negative effect on E. fetida in terms of weight loss. - Biochar decreased PAH biovailability but was less effective at reducing PTE mobility, whilst E. fetida increased both PAH and PTE bioavailability.

Cloning, expression and characterization of a gene from earthworm Eisenia fetida encoding a blood-clot dissolving protein.

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GangQiang Li

Full Text Available A lumbrokinase gene encoding a blood-clot dissolving protein was cloned from earthworm (Eisenia fetida by RT-PCR amplification. The gene designated as CST1 (GenBank No. AY840996 was sequence analyzed. The cDNA consists of 888 bp with an open reading frame of 729 bp, which encodes 242 amino acid residues. Multiple sequence alignments revealed that CST1 shares similarities and conserved amino acids with other reported lumbrokinases. The amino acid sequence of CST1 exhibits structural features similar to those found in other serine proteases, including human tissue-type (tPA, urokinase (uPA, and vampire bat (DSPAα1 plasminogen activators. CST1 has a conserved catalytic triad, found in the active sites of protease enzymes, which are important residues involved in polypeptide catalysis. CST1 was expressed as inclusion bodies in Escherichia coli BL21(DE3. The molecular mass of recombinant CST1 (rCST was 25 kDa as estimated by SDS-PAGE, and further confirmed by Western Blot analysis. His-tagged rCST1 was purified and renatured using nickel-chelating resin with a recovery rate of 50% and a purity of 95%. The purified, renatured rCST1 showed fibrinolytic activity evaluated by both a fibrin plate and a blood clot lysis assay. rCST1 degraded fibrin on the fibrin plate. A significant percentage (65.7% of blood clot lysis was observed when blood clot was treated with 80 mg/mL of rCST1 in vitro. The antithrombotic activity of rCST1 was 912 units/mg calculated by comparison with the activity of a lumbrokinase standard. These findings indicate that rCST1 has potential as a potent blood-clot treatment. Therefore, the expression and purification of a single lumbrokinase represents an important improvement in the use of lumbrokinases.

Fate and O-methylating detoxification of Tetrabromobisphenol A (TBBPA) in two earthworms (Metaphire guillelmi and Eisenia fetida).

Science.gov (United States)

Chen, Xian; Gu, Jianqiang; Wang, Yongfeng; Gu, Xueyuan; Zhao, Xiaopeng; Wang, Xiaorong; Ji, Rong

2017-08-01

Tetrabromobisphenol A (TBBPA) is the world's most widely used brominated flame retardant but there is growing concern about its fate and toxicity in terrestrial organisms. In this study, two ecologically different earthworms, Metaphire guillelmi and Eisenia fetida, were exposed to soil spiked with 14 C-labeled TBBPA for 21 days. M. guillelmi accumulated more TBBPA than E. fetida, evidenced by a 2.7-fold higher 14 C-uptake rate and a 1.3-fold higher biota-soil accumulation factor. Considerable amounts of bound residues (up to 40% for M. guillelmi and 18% for E. fetida) formed rapidly in the bodies of both earthworms. 14 C accumulated mostly in the gut of M. guillemi and in the skin of E. fetida, suggesting that its uptake by M. guillelmi was mainly via gut processes whereas in E. fetida epidermal adsorption predominated. The TBBPA transformation potential was greater in M. guillelmi than in E. fetida, since only 5% vs. 34% of extractable 14 C remained as the parent compound after 21 days of exposure. Besides polar metabolites, the major metabolites in both earthworms were TBBPA mono- and dimethyl ethers (O-methylation products of TBBPA). Acute toxicity assessments using filter paper and natural soil tests showed that the methylation metabolites were much less toxic than the parent TBBPA to both earthworms. It indicated that earthworms used O-methylation to detoxify TBBPA, and M. guillelmi exhibited the higher detoxification ability than E. fetida. These results imply that if only the free parent compound TBBPA is measured, not only bioaccumulation may be underestimated but also its difference between earthworm species may be misestimated. The species-dependent fate of TBBPA may provide a better indicator of the differing sensitivities of earthworms to this environmental contaminant. Copyright © 2017 Elsevier Ltd. All rights reserved.

Evaluación del Fertilizante Orgánico Líquido de Lombriz San Rafael en el Cultivo de Rosa cv. Classy Evaluation of the Worm Organic Liquid Fertilizer San Rafael on cv. Classy Rose Crop

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Fernando Ramírez Castañeda

2011-12-01

Full Text Available La aplicación de fertilizantes es una de las principales actividades técnicas que se realizan en el cultivo de rosas, práctica que puede llegar a causar un impacto ambiental importante. El uso de fertilizantes orgánicos en este cultivo ha sido mínimo. Fertilizantes orgánicos como los vermicompuestos aportan elementos, algunos en formas más disponibles, como también mejoran las características físicas de los suelos. El objetivo fue evaluar la adición del fertilizante orgánico líquido de lombriz San Rafael a la fertilización comercial completa y la reducción de un 25% de su composición por cama, midiendo su efecto semanalmente sobre la productividad y la calidad de los tallos florales, el descabece de flor y en análisis foliares en plantas de rosa cv. Classy. Los resultados indican que para los análisis foliares y los diferentes grados de calidad (longitud de tallo y de botón floral no existió evidencia de diferencias estadísticas entre tratamientos. Se encontró diferencia significativa en productividad, siendo mayor el promedio de las plantas tratadas con la fórmula de fertilización completa más el fertilizante orgánico. Para el descabece de la flor se encontró que el tratamiento donde se aplicó la fórmula de fertilización reducida más el fertilizante orgánico, fue significativamente menor en comparación con los otros dos tratamientos.Fertilizer application is one of the main technical activities made in rose crop, but it may cause an important environmental impact. The use of organic fertilizers has been low in this crop. An organic fertilizer as vermicompost contributes with some elements in better available forms, and improves some soil physical characteristics. The purpose was to evaluate the addition of worm liquid organic fertilizer San Rafael to complete commercial fertilization and to decrease 25% of its composition per bed, measuring weekly its effect on yield, degrees of quality flowering stems

Optimization of Eisenia fetida stocking density for the bioconversion of rock phosphate enriched cow dung–waste paper mixtures

International Nuclear Information System (INIS)

Unuofin, F.O.; Mnkeni, P.N.S.

2014-01-01

Highlights: • Vermidegradation of RP-enriched waste mixtures is dependent on E. fetida stocking density. • A stocking density of 12.5 g-worms kg -1 resulted in highly humified vermicomposts. • P release from RP-enriched waste vermicomposts increases with E. fetida stocking density. • RP-enriched waste vermicomposts had no inhibitory effect on seed germination. - Abstract: Vermitechnology is gaining recognition as an environmental friendly waste management strategy. Its successful implementation requires that the key operational parameters like earthworm stocking density be established for each target waste/waste mixture. One target waste mixture in South Africa is waste paper mixed with cow dung and rock phosphate (RP) for P enrichment. This study sought to establish optimal Eisenia fetida stocking density for maximum P release and rapid bioconversion of RP enriched cow dung–paper waste mixtures. E. fetida stocking densities of 0, 7.5, 12.5, 17.5 and 22.5 g-worms kg −1 dry weight of cow dung–waste paper mixtures were evaluated. The stocking density of 12.5 g-worms kg −1 resulted in the highest earthworm growth rate and humification of the RP enriched waste mixture as reflected by a C:N ratio of <12 and a humic acid/fulvic acid ratio of >1.9 in final vermicomposts. A germination test revealed that the resultant vermicompost had no inhibitory effect on the germination of tomato, carrot, and radish. Extractable P increased with stocking density up to 22.5 g-worm kg −1 feedstock suggesting that for maximum P release from RP enriched wastes a high stocking density should be considered

Optimization of Eisenia fetida stocking density for the bioconversion of rock phosphate enriched cow dung–waste paper mixtures

Energy Technology Data Exchange (ETDEWEB)

Unuofin, F.O., E-mail: funmifrank2009@gmail.com; Mnkeni, P.N.S., E-mail: pmnkeni@ufh.ac.za

2014-11-15

Highlights: • Vermidegradation of RP-enriched waste mixtures is dependent on E. fetida stocking density. • A stocking density of 12.5 g-worms kg{sup -1} resulted in highly humified vermicomposts. • P release from RP-enriched waste vermicomposts increases with E. fetida stocking density. • RP-enriched waste vermicomposts had no inhibitory effect on seed germination. - Abstract: Vermitechnology is gaining recognition as an environmental friendly waste management strategy. Its successful implementation requires that the key operational parameters like earthworm stocking density be established for each target waste/waste mixture. One target waste mixture in South Africa is waste paper mixed with cow dung and rock phosphate (RP) for P enrichment. This study sought to establish optimal Eisenia fetida stocking density for maximum P release and rapid bioconversion of RP enriched cow dung–paper waste mixtures. E. fetida stocking densities of 0, 7.5, 12.5, 17.5 and 22.5 g-worms kg{sup −1} dry weight of cow dung–waste paper mixtures were evaluated. The stocking density of 12.5 g-worms kg{sup −1} resulted in the highest earthworm growth rate and humification of the RP enriched waste mixture as reflected by a C:N ratio of <12 and a humic acid/fulvic acid ratio of >1.9 in final vermicomposts. A germination test revealed that the resultant vermicompost had no inhibitory effect on the germination of tomato, carrot, and radish. Extractable P increased with stocking density up to 22.5 g-worm kg{sup −1} feedstock suggesting that for maximum P release from RP enriched wastes a high stocking density should be considered.

{sup 1}H NMR-based metabolomics of time-dependent responses of Eisenia fetida to sub-lethal phenanthrene exposure

Energy Technology Data Exchange (ETDEWEB)

Lankadurai, Brian P.; Wolfe, David M.; Simpson, Andre J. [Department of Chemistry, University of Toronto, 1265 Military Trail, Toronto, Ontario M1C 1A4 Canada (Canada); Simpson, Myrna J., E-mail: myrna.simpson@utoronto.ca [Department of Chemistry, University of Toronto, 1265 Military Trail, Toronto, Ontario M1C 1A4 Canada (Canada)

2011-10-15

{sup 1}H NMR-based metabolomics was used to examine the response of the earthworm Eisenia fetida after exposure to sub-lethal concentrations of phenanthrene over time. Earthworms were exposed to 0.025 mg/cm{sup 2} of phenanthrene (1/64th of the LC{sub 50}) via contact tests over four days. Earthworm tissues were extracted using a mixture of chloroform, methanol and water, resulting in polar and non-polar fractions that were analyzed by {sup 1}H NMR after one, two, three and four days. NMR-based metabolomic analyses revealed heightened E. fetida responses with longer phenanthrene exposure times. Amino acids alanine and glutamate, the sugar maltose, the lipids cholesterol and phosphatidylcholine emerged as potential indicators of phenanthrene exposure. The conversion of succinate to fumarate in the Krebs cycle was also interrupted by phenanthrene. Therefore, this study shows that NMR-based metabolomics is a powerful tool for elucidating time-dependent relationships in addition to the mode of toxicity of phenanthrene in earthworm exposure studies. - Highlights: > NMR-based earthworm metabolomic analysis of the mode of action of phenanthrene is presented. > The earthworm species E. fetida were exposed to sub-lethal phenanthrene concentrations. > Both polar and non-polar metabolites of E. fetida tissue extracts were analyzed by {sup 1}H NMR. > Longer phenanthrene exposure times resulted in heightened earthworm responses. > An interruption of the Krebs cycle was also observed due to phenanthrene exposure. - {sup 1}H NMR metabolomics is used to determine the relationship between phenanthrene exposure and the metabolic response of the earthworm E. fetida over time and also to elucidate the phenanthrene mode of toxicity.

Growth and reproductive potential of Eisenia foetida (Sav) on various zoo animal dungs after two methods of pre-composting followed by vermicomposting.

Science.gov (United States)

Pérez-Godínez, Edmundo Arturo; Lagunes-Zarate, Jorge; Corona-Hernández, Juan; Barajas-Aceves, Martha

2017-06-01

Disposal of animal manure without treatment can be harmful to the environment. In this study, samples of four zoo animal dungs and one horse dung were pre-composted in two ways: (a) traditional composting and (b) bokashi pre-composting for 1month, followed by vermicomposting for 3months. The permanence (PEf) and reproductive potential (RP) of Eisenia foetida as well as the quality of vermicompost were evaluated. The PEf values and RP index of E. foetida were higher for samples pre-composted using the traditional composting method (98.7-88% and 31.85-16.27%, respectively) followed by vermicomposting (92.7-72.7% and 22.96-13.51%, respectively), when compared with those for bokashi pre-composted samples followed by vermicomposting, except for the horse dung sample (100% for both the parameters). The values of electrical conductivity (EC), cation exchange capacity (CEC), organic C, total N, available P, C/N ratio, and pH showed that both treatments achieved the norms of vermicompost (bokashi pre-composting followed by vermicomposting produced the highest values (98.7-70.7%, 97.67-96.65%, and 2.7-1.97%, respectively), when compared with the other method adapted in this study. Nevertheless, further studies with plants for plant growth evaluation are needed to assess the benefits and limitations of these two pre-composting methods prior to vermicomposting. Copyright © 2017 Elsevier Ltd. All rights reserved.

Potentiality of Eisenia fetida to degrade disposable paper cups-an ecofriendly solution to solid waste pollution.

Science.gov (United States)

Arumugam, Karthika; Ganesan, Seethadevi; Muthunarayanan, Vasanthy; Vivek, Swabna; Sugumar, Susila; Munusamy, Vivekanadhan

2015-02-01

The aim of the present study was to subject the post-consumer waste, namely paper cups for vermicomposting along with cow dung in three different ratios for a period of 90-140 days employing Eisenia fetida. The post-consumer wastes are a menace in many developing countries including India. This waste was provided as feed for earthworms and was converted to vermicompost. Vermicompost prepared with paper cup waste was analyzed for their physicochemical properties. Based on the physicochemical properties, it was evident that the best manure is obtained from type A (paper cup/cow dung in the ratio 1:1) than type B (paper cup/cow dung in the ratio 1.5:0.5) and type C (paper cup/cow dung in the ratio 0.5:1.5). The results showed that earthworms accelerated the rate of mineralization and converted the wastes into compost with needed elements which could support the growth of crop plants. The predominant bacterial strains in the vermicompost were characterized biochemically as well as by 16S ribosomal RNA (rRNA) gene sequencing. The bacterial strains like Bacillus anthracis (KM289159), Bacillus endophyticus (KM289167), Bacillus funiculus (KM289165), Virigibacillius chiquenigi (KM289163), Bacillus thuringiensis (KM289164), Bacillus cereus (KM289160), Bacillus toyonensis (KM289161), Acinetobacter baumanni (KM289162), and Lactobacillus pantheries (KM289166) were isolated and identified from the final compost. The total protein content of E. fetida involved in vermicomposting was extracted, and the banding pattern was analyzed. During final stages of vermicomposting, it was observed that the earthworm did not act on the plastic material coated inside the paper cups and stagnated it around the rim of the tub. Further, the degradation of paper cup waste was confirmed by Fourier transform infrared spectroscopy analysis. Hence, vermicomposting was found to be an effective technology for the conversion of the paper cup waste material into a nutrient-rich manure, a value

Use of agave bagasse for production of an organic fertilizer by pretreatment with Bjerkandera adusta and vermicomposting with Eisenia fetida.

Science.gov (United States)

Moran-Salazar, Rene G; Marino-Marmolejo, Erika N; Rodriguez-Campos, Jacobo; Davila-Vazquez, Gustavo; Contreras-Ramos, Silvia M

2016-01-01

Agave tequilana Weber is used in tequila and fructans production, with agave bagasse generated as a solid waste. The main use of bagasse is to produce compost in tequila factories with a long traditional composting that lasts 6-8 months. The aim of this study was to evaluate the degradation of agave bagasse by combining a pretreatment with fungi and vermicomposting. Experiments were carried out with fractionated or whole bagasse, sterilized or not, subjecting it to a pretreatment with Bjerkandera adusta alone or combined with native fungi, or only with native bagasse fungi (non-sterilized), for 45 days. This was followed by a vermicomposting with Eisenia fetida and sewage sludge, for another 45 days. Physicochemical parameters, lignocellulose degradation, stability and maturity changes were measured. The results indicated that up to 90% of the residual sugars in bagasse were eliminated after 30 days in all treatments. The highest degradation rate in pretreatment was observed in non-sterilized, fractionated bagasse with native fungi plus B. adusta (BNFns) (71% hemicellulose, 43% cellulose and 71% lignin) at 45 days. The highest total degradation rates after vermicomposting were in fractionated bagasse pre-treated with native fungi (94% hemicellulose, 86% cellulose and 91% lignin). However, the treatment BNFns showed better maturity and stability parameters compared to that reported for traditional composts. Thus, it seems that a process involving vermicomposting and pretreatment with B. adusta could reduce the degradation time of bagasse to 3 months, compared to the traditional composting process, which requires from 6 to 8 months.

The effects of the insecticide lambda-Cyhalothrin on the earthworm Eisenia fetida under experimental conditions of tropical and temperate regions

International Nuclear Information System (INIS)

Garcia, Marcos; Scheffczyk, Adam; Garcia, Terezinha; Roembke, Joerg

2011-01-01

Plant Protection Products can affect soil organisms and thus might have negative impacts on soil functions. Little research has been performed on their impact on tropical soils. Therefore, the effects of the insecticide lambda-Cyhalothrin on earthworms were evaluated in acute and chronic laboratory tests modified for tropical conditions, i.e. at selected temperatures (20 and 28 o C) and with two strains (temperate and tropical) of the compost worm Eisenia fetida. The insecticide was spiked in two natural soils, in OECD artificial soil and a newly developed tropical artificial soil. The effects of lambda-Cyhalothrin did rarely vary in the same soil at tropical (LC50: 68.5-229 mg a.i./kg dry weight (DW); EC50: 54.2-60.2 mg a.i./kg DW) and temperate (LC50: 99.8-140 mg a.i./kg DW; EC50: 37.4-44.5 mg a.i./kg DW) temperatures. In tests with tropical soils and high temperature, effect values differed by up to a factor of ten. - Research highlights: → In one soil, effects of lambda-Cyhalothrin did not vary much at two temperatures. → In tropical soils at high temperature, effects differed by up to a factor of ten. → In the tropics, effects of pesticides can be higher or lower as in temperate regions. - The effects of the insecticide lambda-cyhalothrin on earthworms did not differ considerably when performed in the same soil under different temperatures, but LC/EC 50 values varied by a factor of ten between OECD and tropical artificial soil.

The effects of the insecticide lambda-Cyhalothrin on the earthworm Eisenia fetida under experimental conditions of tropical and temperate regions

Energy Technology Data Exchange (ETDEWEB)

Garcia, Marcos [Embrapa Amazonia Ocidental, Rod. AM-10, Km 28, 69.011-970 Manaus, AM (Brazil); Scheffczyk, Adam [ECT Oekotoxikologie, Boettgerstr. 2-14, D-65439 Floersheim (Germany); Garcia, Terezinha [Embrapa Amazonia Ocidental, Rod. AM-10, Km 28, 69.011-970 Manaus, AM (Brazil); Roembke, Joerg, E-mail: j-roembke@ect.d [ECT Oekotoxikologie, Boettgerstr. 2-14, D-65439 Floersheim (Germany)

2011-02-15

Plant Protection Products can affect soil organisms and thus might have negative impacts on soil functions. Little research has been performed on their impact on tropical soils. Therefore, the effects of the insecticide lambda-Cyhalothrin on earthworms were evaluated in acute and chronic laboratory tests modified for tropical conditions, i.e. at selected temperatures (20 and 28 {sup o}C) and with two strains (temperate and tropical) of the compost worm Eisenia fetida. The insecticide was spiked in two natural soils, in OECD artificial soil and a newly developed tropical artificial soil. The effects of lambda-Cyhalothrin did rarely vary in the same soil at tropical (LC50: 68.5-229 mg a.i./kg dry weight (DW); EC50: 54.2-60.2 mg a.i./kg DW) and temperate (LC50: 99.8-140 mg a.i./kg DW; EC50: 37.4-44.5 mg a.i./kg DW) temperatures. In tests with tropical soils and high temperature, effect values differed by up to a factor of ten. - Research highlights: In one soil, effects of lambda-Cyhalothrin did not vary much at two temperatures. In tropical soils at high temperature, effects differed by up to a factor of ten. In the tropics, effects of pesticides can be higher or lower as in temperate regions. - The effects of the insecticide lambda-cyhalothrin on earthworms did not differ considerably when performed in the same soil under different temperatures, but LC/EC{sub 50} values varied by a factor of ten between OECD and tropical artificial soil.

Bioaccumulation of Zn and Ag Nanoparticles in the Earthworms (Eisenia fetida)

Science.gov (United States)

Ha, Lee Seung; Sung-Dae, Kim; Yi, Yang Song; Byeong-Gweon, Lee

2014-05-01

Many studies are carried out to evaluate environmental effects of engineered nanoparticles (ENPs). Most of the previous studies primarily focused on the effects of nanoparticles into the aquatic environment and human. Model studies predict that ENPs released into environment would transferred primarily to the soil of the terrestrial environment. Despite this prediction, biogeochemical behavior of ENPs in soil environment as well as bioavailability of ENPs to soil-dwelling organisms such as earthworm, springtail, isopod and nematodes are poorly understood. The main goal of this study was to compare the bioaccumulation factor (BAFs) and subcellular partitioning of nanoparticles in the soil-dwelling earthworm (Eisenia fetida) from ENP (ZnO and Ag nanoparticles) or ionic metal (Zn2+, Ag+) contaminated soil. And the sequential extraction was also used to determine the mobility of metals in soil which could be used as to predict bioavailability and compare that with bioaccumulation factor. The radiotracer method was employed to trace the transfer of ENPs and ionic metal among different environmental media and animals. Radiolabeled 65ZnO, 110mAgNPs coated with PVP or citrate were synthesized in the laboratory and their chemical and biological behavior was compared to ionic 65Zn and 110mAg. The BAFs of Zn and Ag in the earthworms were determined after animals exposed to the contaminated soils. After the 7 days of elimination phase, subcellular partitioning of metals were also obtained. BAF for ZnO(0.06) was 31 times lower than that for Zn ion (1.86), suggesting that ZnO was less bioavailable than its ionic form from contaminated soil. On the other hands, BAFs for AgNPs coated with PVP (0.12) or with citrate (0.11) were comparable to those for Ag ion (0.17), indicating that Ag from contaminated soil was bioavailable in a similar rate regardless of chemical forms. The subcellular partitioning results showed that bioaccumulated Zn from Zn ion and ZnO contaminated soil were

Enrofloxacin at environmentally relevant concentrations enhances uptake and toxicity of cadmium in the earthworm Eisenia fetida in farm soils

Energy Technology Data Exchange (ETDEWEB)

Li, Yinsheng, E-mail: yinshengli@sjtu.edu.cn [School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240 (China); Tang, Hao; Hu, Yingxiu; Wang, Xiuhong; Ai, Xiaojie; Tang, Li [School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240 (China); Matthew, Cory [Institute of Agriculture & Environment, Massey University, Private Bag 11-222, Palmerston North 4442 (New Zealand); Cavanagh, Jo [Landcare Research, PO Box 40, Lincoln 7640 (New Zealand); Qiu, Jiangping [School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240 (China)

2016-05-05

Highlights: • Enrofloxacin (EF) and cadmium (Cd) were independently adsorbed in soils. • EF accelerated and increased Cd bioaccumulation in earthworms. • At high concentration EF (10 mg kg{sup −1}) was toxic to earthworms. • EF enhanced Cd induced oxidative stress, and increased burrowing and respiration. • EF did not affect the Cd induced increase in metallothionein in earthworms. - Abstract: Individual and combined effects of enrofloxacin (EF) and cadmium (Cd) on the earthworm Eisenia fetida at environmentally relevant concentrations were investigated. EF is a veterinary antibiotic; Cd is an impurity in phosphatic fertiliser. For both, residues may accumulate in farm soils. In laboratory tests, over 98% of spiked EF was adsorbed by farm soils, with a half-life >8 weeks. However, earthworms absorbed less than 20% of spiked EF. Earthworms in soil with EF concentration 10 mg kg{sup −1} soil experienced transient oxidative stress and exhibited reduced burrowing activity and respiration after an 8-week exposure; EF at 0.1 and 1.0 mg kg{sup −1} soil did not elicit toxicity symptoms. When both were added, Cd did not affect EF uptake, but each increment of spiked EF increased Cd bioaccumulation and associated oxidative stress of earthworms, and also caused decreased burrow length and CO{sub 2} production. However, metallothionein induction was not affected. The enhanced toxicity of Cd to earthworms in the presence of EF at low environmental concentrations may have implications for the health and reproductive success of earthworm populations and highlights the importance of understanding effects of antibiotic contamination of farm soils, and of awareness of environmental effects from interaction between multiple contaminants.

Enrofloxacin at environmentally relevant concentrations enhances uptake and toxicity of cadmium in the earthworm Eisenia fetida in farm soils

International Nuclear Information System (INIS)

Li, Yinsheng; Tang, Hao; Hu, Yingxiu; Wang, Xiuhong; Ai, Xiaojie; Tang, Li; Matthew, Cory; Cavanagh, Jo; Qiu, Jiangping

2016-01-01

Highlights: • Enrofloxacin (EF) and cadmium (Cd) were independently adsorbed in soils. • EF accelerated and increased Cd bioaccumulation in earthworms. • At high concentration EF (10 mg kg"−"1) was toxic to earthworms. • EF enhanced Cd induced oxidative stress, and increased burrowing and respiration. • EF did not affect the Cd induced increase in metallothionein in earthworms. - Abstract: Individual and combined effects of enrofloxacin (EF) and cadmium (Cd) on the earthworm Eisenia fetida at environmentally relevant concentrations were investigated. EF is a veterinary antibiotic; Cd is an impurity in phosphatic fertiliser. For both, residues may accumulate in farm soils. In laboratory tests, over 98% of spiked EF was adsorbed by farm soils, with a half-life >8 weeks. However, earthworms absorbed less than 20% of spiked EF. Earthworms in soil with EF concentration 10 mg kg"−"1 soil experienced transient oxidative stress and exhibited reduced burrowing activity and respiration after an 8-week exposure; EF at 0.1 and 1.0 mg kg"−"1 soil did not elicit toxicity symptoms. When both were added, Cd did not affect EF uptake, but each increment of spiked EF increased Cd bioaccumulation and associated oxidative stress of earthworms, and also caused decreased burrow length and CO_2 production. However, metallothionein induction was not affected. The enhanced toxicity of Cd to earthworms in the presence of EF at low environmental concentrations may have implications for the health and reproductive success of earthworm populations and highlights the importance of understanding effects of antibiotic contamination of farm soils, and of awareness of environmental effects from interaction between multiple contaminants.

Efecto de mallas sombreadoras sobre la producción y calidad de frutos de arándano (Vaccinium corymbosum L. cv. Brigitta

Directory of Open Access Journals (Sweden)

Mario Marcelo Rodríguez Beraud

2015-01-01

Full Text Available Se evaluó el crecimiento, desarrollo y calidad de frutos de arándano (Vaccinium corymbosum L. variedad Brigitta bajo diversas mallas de sombreo. Los ensayos se realizaron en plantas de 7 años en un huerto comercial de Collipulli, Región de La Araucanía, Chile. Los tratamientos correspondieron a un control sin sombreamiento y cuatro tipos de mallas: una roja de 40% y 18% de sombreamiento; una malla aluminizada con 40% de sombreamiento y una malla negra de 35% de sombreamiento. En frutos bajo sombra de malla roja 40% se observaron el mayor diámetro ecuatorial y polar y se cosecharon tres semanas más tarde que los frutos del tratamiento sin malla. En el cultivo bajo sombra de malla aluminizada 40% se observó prolongación de estados fenológicos y un desfase en la producción máxima semanal de dos semanas respecto al control. Los mayores rendimientos se observaron en plantas del tratamiento control y bajo sombra de malla roja 40%, con 11008 kg ha-1 y 10461 kg ha-1 respectivamente, en tanto que el menor rendimiento fue con malla roja 18% con 9668 kg ha-1. Los frutos cultivados bajo sombra de malla roja 18% presentaron el mayor número de frutos por planta con 1806 bayas por planta, el menor peso de frutos con 1,69 g y la mayor firmeza. Por lo tanto, el mayor peso de bayas se observa en frutos sin malla y en malla roja 40% de sombreamiento, junto con el mayor tamaño y rendimiento.

The Lower Cretaceous Way Group of northern Chile: An alluvial fan-fan delta complex

Science.gov (United States)

Flint, S.; Clemmey, H.; Turner, P.

1986-01-01

Alluvial fan sediments of the Lower Cretaceous Coloso Basin in northern Chile were deposited in a half-graben and derived from andesitic volcanics of a former island arc. Transport directions were towards the east, away from the present-day Peru-Chile trench. Grain flow, density modified grain flow and sheetflow processes were responsible for most of the sediment deposition with cohesive debris flows playing only a minor part. An early phase of conglomerate deposition (Coloso Formation) into a restricted basin records the transition from proximal fan facies with abundant grain flows and remobilized screes to mid-fan facies dominated by sheetflows. Stratiform copper mineralization near the top of the lower conglomerates is related to the unroofing of the Jurassic island arc. This mineralization comprises copper sulphide-cemented sands and gravels and formed by the reaction of mineralized detritus with diagenetic and hydrothermal solutions. A later phase of deposition (Lombriz Formation) includes sandstones, siltstones and conglomerates with a source area different from the Coloso Formation. This change in source may be related to strike-slip tectonics as the basin extended. The Lombriz conglomerates pass distally (eastwards) into red sandstones and purple siltstones with thin limestones deposited under marine conditions. This sequence is interpreted as a major fan delta complex. It passes conformably into marine carbonates of the Tableado Formation signifying the complete drowning of the basin in lower Cretaceous times.

EPR detection of hydroxyl radical generation and oxidative perturbations in lead-exposed earthworms (Eisenia fetida) in the presence of decabromodiphenyl ether.

Science.gov (United States)

Liu, Kou; Chen, Lin; Zhang, Wei; Lin, Kuangfei; Zhao, Li

2015-03-01

Lead (Pb) and decabromodiphenyl ether (BDE209) are the main contaminants at e-waste recycling sites, and their potential toxicological effects on terrestrial organisms have received extensive attention. However, the impacts on the oxidative perturbations and hydroxyl radical (·OH) generation in earthworms of exposure to the two chemicals remain almost unknown. Therefore, indoor incubation tests were performed on control and contaminated soil samples to determine the effects of Pb in earthworms Eisenia fetida in the presence of BDE209 through the use of several biomarkers in microcosms. The results have demonstrated that the addition of BDE209 (1 or 10 mg kg(-1)) decreased the enzymatic activities [superoxide dismutase, catalase (CAT), peroxidase] and total antioxidant capacity (T-AOC) compared with exposure to BDE209 alone (50, 250 or 500 mg kg(-1)). Electron paramagnetic resonance spectra indicated that ·OH radicals in earthworms were significantly induced by Pb in the presence of BDE209. The changing pattern of malondialdehyde (MDA) contents was accordant with that of ·OH intensity suggested that reactive oxygen species might lead to cellular lipid peroxidation. Furthermore, CAT exhibited more sensitive response to single Pb exposure than the other biomarkers, while T-AOC, ·OH and MDA might be three most sensitive biomarkers in earthworms after simultaneous exposure to Pb and BDE209. The results of these observations suggested that oxidative stress appeared in E. fetida, and it may play an important role in inducing the Pb and BDE209 toxicity to earthworms.

Impacts of BDE209 addition on Pb uptake, subcellular partitioning and gene toxicity in earthworm (Eisenia fetida)

Energy Technology Data Exchange (ETDEWEB)

Zhang, Wei, E-mail: wzhang@ecust.edu.cn [State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237 (China); School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237 (China); Liu, Kou; Li, Jing; Liang, Jun; Lin, Kuangfei [State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237 (China); School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237 (China)

2015-12-30

Highlights: • 10 or 100 μg g{sup −1} BDE209 addition caused histological changes in Pb-exposed earthworms’ body wall. • Strong histopathological effects with BDE209 addition confirmed the enhanced Pb bioavailability. • The presence of higher levels of BDE209 altered subcellular partitioning of Pb in earthworm. • Co-exposure to Pb and BDE209 declined SOD and CAT gene transcripts synergistically. • BDE209 addition elicited up-regulation of Hsp90 gene expression compared to Pb exposure alone. - Abstract: Lead (Pb) and decabromodiphenyl ether (BDE209) are the mainly co-existed contaminants at e-waste recycling sites. The potential toxicity of Pb (250 μg g{sup −1}) to earthworm Eisenia fetida in the presence of BDE209 (1, 10 and 100 μg g{sup −1}) was determined during 14-d incubation period. Compared to Pb treatment alone, the co-exposure with 1 μg g{sup −1} BDE209 barely affected Pb uptake, subcellular partitioning and gene expression; however, histopathological changes in earthworms’ body wall (epidermal, circular and longitudinal muscles) demonstrated that 10 and 100 μg g{sup −1} BDE209 additions enhanced Pb uptake and altered its subcellular partitioning, indicating that Pb redistributed from fractions E (cell debris) and D (metal-rich granules) to fraction C (cytosols); Additionally, BDE209 supply significantly inhibited (p < 0.05) the induction of SOD (superoxide dismutase) and CAT (catalase) gene expressions (maximum down-regulation 59% for SOD gene at Pb + 100 μg g{sup −1} BDE209 and 89% for CAT gene at Pb + 10 μg g{sup −1} BDE209), while facilitated (p < 0.05) Hsp90 (heat shock protein 90) gene expression with maximum induction rate of 120% after exposure to Pb + 10 μg g{sup −1} BDE209. These findings illustrate the importance of considering environmental BDE209 co-exposure when assessing Pb bioaccumulation and toxicity in multi-contaminated soil ecosystems.

Effects of Different Ratios of Sewage Sludge and Cattle Manure on Growth and Propagation of Eisenia Fetida.

Directory of Open Access Journals (Sweden)

Yukui Li

Full Text Available Domestic sewage sludge and cattle manure are rich in nutrition elements, but without proper disposal, are harmful to the environment. Here with an indoor culture method, we used Eisenia fetida to dispose different ratios of sewage sludge and cattle manure, and thereby investigated the effects and acting rules of these sludge-manure mixtures on the growth and reproduction of E. fetida. We find these mixtures are food sources for E. fetida, and their physiochemical properties are significantly changed after disposal by earthworms. Paired samples t-test shows the average change after different treatments is -20.37% for total organic carbon, 85.71% for total Kjeldahl N, -6.67% for total P, 8.33% for pH, -24.78% for EC (ms·cm-1, and -57.10% for C/N ratio. The average growth rate after treatment CD-70 is 9.20 mg·worm-1·day-1; the average growth rates of E. fetida on day 0-28, day 29-56, and day 57-91 are 9.33, 11.90 and 6.95 mg·worm-1·day-1, respectively, indicating a trend of "rapid-rapidest-slow" growth. Other treatments all show this trend. Though all earthworms developed reproductive rings during the test periods, the appearing time and the cocoon production time both differed among these treatments. The cocoon production amount is maximized to 233 after treatment CD-70. The cocoon production rates are significantly different among these treatments, and the maximum and mean are 0.32 and 0.17-0.32, cocoons·worm-1· day-1, respectively. E. fetida can modestly enrich Cd, but is not very effective over Sb or other heavy metals. E. fetida can remove a part of heavy metals from sewage sludge and cattle manure. Generally, the mixtures of sewage sludge and cattle manure can largely affect the growth and propagation of E. fetida in a ratio-dependent way.

Impacts of BDE209 addition on Pb uptake, subcellular partitioning and gene toxicity in earthworm (Eisenia fetida)

International Nuclear Information System (INIS)

Zhang, Wei; Liu, Kou; Li, Jing; Liang, Jun; Lin, Kuangfei

2015-01-01

Highlights: • 10 or 100 μg g −1 BDE209 addition caused histological changes in Pb-exposed earthworms’ body wall. • Strong histopathological effects with BDE209 addition confirmed the enhanced Pb bioavailability. • The presence of higher levels of BDE209 altered subcellular partitioning of Pb in earthworm. • Co-exposure to Pb and BDE209 declined SOD and CAT gene transcripts synergistically. • BDE209 addition elicited up-regulation of Hsp90 gene expression compared to Pb exposure alone. - Abstract: Lead (Pb) and decabromodiphenyl ether (BDE209) are the mainly co-existed contaminants at e-waste recycling sites. The potential toxicity of Pb (250 μg g −1 ) to earthworm Eisenia fetida in the presence of BDE209 (1, 10 and 100 μg g −1 ) was determined during 14-d incubation period. Compared to Pb treatment alone, the co-exposure with 1 μg g −1 BDE209 barely affected Pb uptake, subcellular partitioning and gene expression; however, histopathological changes in earthworms’ body wall (epidermal, circular and longitudinal muscles) demonstrated that 10 and 100 μg g −1 BDE209 additions enhanced Pb uptake and altered its subcellular partitioning, indicating that Pb redistributed from fractions E (cell debris) and D (metal-rich granules) to fraction C (cytosols); Additionally, BDE209 supply significantly inhibited (p < 0.05) the induction of SOD (superoxide dismutase) and CAT (catalase) gene expressions (maximum down-regulation 59% for SOD gene at Pb + 100 μg g −1 BDE209 and 89% for CAT gene at Pb + 10 μg g −1 BDE209), while facilitated (p < 0.05) Hsp90 (heat shock protein 90) gene expression with maximum induction rate of 120% after exposure to Pb + 10 μg g −1 BDE209. These findings illustrate the importance of considering environmental BDE209 co-exposure when assessing Pb bioaccumulation and toxicity in multi-contaminated soil ecosystems.

Responses of growth inhibition and antioxidant gene expression in earthworms (Eisenia fetida) exposed to tetrabromobisphenol A, hexabromocyclododecane and decabromodiphenyl ether.

Science.gov (United States)

Shi, Ya-juan; Xu, Xiang-bo; Zheng, Xiao-qi; Lu, Yong-long

2015-01-01

Tetrabromobisphenol A (TBBPA), hexabromocyclododecane (HBCD) and decabromodiphenyl ether (BDE 209), suspected ubiquitous contaminants, account for the largest volume of brominated flame retardants (BFRs) since penta-BDE and octa-BDE have been phased out globally. In this paper, the growth inhibition and gene transcript levels of antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT)) and the stress-response gene involved in the prevention of oxidative stress (Hsp70) of earthworms (Eisenia fetida) exposed to TBBPA, HBCD and BDE 209 were measured to identify the toxicity effects of selected BFRs on earthworms. The growth of earthworms treated by TBBPA at 200 and 400 mg/kg dw were inhibited at rate of 13.7% and 22.0% respectively, while there was no significant growth inhibition by HBCD and BDE 209. A significant (Pearthworms exposed to TBBPA at 50 mg/kg dw (1.77-fold) and to HBCD at 400 mg/kg dw (2.06-fold). The transcript level of Hsp70 gene was significantly up-regulated (Pearthworms exposed to TBBPA at concentration of 50-200 mg/kg (2.16-2.19-fold) and HBCD at 400 mg/kg (2.61-fold). No significant variation of CAT gene expression in all the BFRs treatments was observed, neither does all the target gene expression level exposed to BDE 209. Assessed by growth inhibition and the changes at mRNA levels of encoding genes in earthworms, TBBPA showed the greatest toxicity, followed by HBCD and BDE 209, consistent with trends in molecular properties. The results help to understand the molecular mechanism of antioxidant defense. Copyright © 2015 Elsevier Inc. All rights reserved.

Evaluación de vermicompostas de origen vegetal para el manejo de ralstonia solanacearum y pectobacterium carotovorum subsp. carotovorum.

OpenAIRE

Flores Ocampo, Mitzi

2016-01-01

La vermicomposta es un tipo de abono orgánico basado en un proceso de biooxidación y estabilización de desechos orgánicos que involucran interacciones entre lombrices y microorganismos del suelo; su potencial como bioplaguicida contra fitopatógenos ha sido poco estudiado pero depende del tipo de materia orgánica con que se alimente a la lombriz. Pectobacterium carotovorum subsp. carotovorum y Ralstonia solanacearum son bacterias que causan enfermedades en Solanáceas, tanto en c...

La yerba no es basura : lombricultura y producción de Vermicompost a partir de residuos de yerba mate en Uruguay

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María Torrendel

2011-05-01

Full Text Available En Uruguay el consumo anual de yerba mate genera unas 125 toneladas de residuos orgánicos. La lombricultura es una delas formas de obtener materia orgánica a partir del reciclado de residuos domiciliarios orgánicos. Los desechos de yerbamate tienen un gran potencial de reciclado si son utilizados como sustrato en la lombricultura. Utilizando distintos sustratos,tiempos de compostaje y volúmenes de yerba, se determinaron algunas de las condiciones necesarias para la utilización de dichos residuos como sustrato para la lombriz Eisenia fetida. Se estudiaron las condiciones de pH que el desecho debería alcanzar para ser tolerado y mejor aprovechado por las lombrices, y el período de tiempo que la yerba demora en alcanzar estas condiciones. Se observó que el período de tiempo mínimo de precompostado para el mejor aprovechamiento de la yerba por parte de las lombrices es de cuatro semanas. Se analizaron también las características del vermicompost obtenido, en cuanto a parámetros de materia orgánica, nitrógeno, fósforo y metales. El valor de nitrógeno (1 % y de materia orgánica, que ronda el 30 %, son similares a los encontrados en otros humus que se obtienen por métodos semejantes. Sin embargo, para el Fósforo los valores resultaron por debajo de lo encontrado en los análisis realizados por el CUI (Laboratorio Ambiental Echotech, octubre 2006.AbstractThe annual consumption of “yerba mate” (mate herb infusion in Uruguay generates about 125 metric tons of organic waste. Vermicomposting is one way to obtain organic matter by recycling organic household waste. The waste of “yerba mate” has a great potential for recycling if it is used as a substrate in vermicomposting. Using different substrates, composting times andvolumes of “yerba mate” we determined some of the necessary conditions for the use of yerba mate waste as a substrate for the Eisenia fetida earthworm. We studied the optimal pH conditions that the waste

Eficiencia de acumulación de Fe, Cu, Zn y Se en lombriz de tierra (Eisenia fetida como base para la elaboración de un suplemento nutricional con oligoelementos

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Lué-Merú Marcó P

2012-05-01

Full Text Available It has been determined the bioaccumulation of Cu, Fe, Zn and Se, in the tissue of earthworms to evaluate the possibility of use as a nutritional supplement. Was analyzed a vermicompost enriched substrate (control and three doses of each element. We studied the effect on growth, mortality and tissue concentrations. Significant differences were found for copper control, However, tissue concentrations for all groups are high compared to some foods from the diet is an alternative to replace and compensate for the deficiency of these trace elements.

Endocrine disruptors in soil: Effects of bisphenol A on gene expression of the earthworm Eisenia fetida.

Science.gov (United States)

Novo, M; Verdú, I; Trigo, D; Martínez-Guitarte, J L

2018-04-15

Xenobiotics such as bisphenol A (BPA), are present in biosolids, which are applied as organic fertilizers in agricultural fields. Their effects on soil life have been poorly assessed, and this is particularly important in the case of earthworms, which represent the main animal biomass in this medium. In the present work we study the impacts of BPA on gene expression of Eisenia fetida, a widely used ecotoxicological model. Chronic soil tests and acute contact tests were performed, and gene expression was analyzed in total tissue and in masculine reproductive organs of the earthworms. The genes studied in this research played a role in endocrine pathways, detoxification mechanisms, stress response, epigenetics, and genotoxicity. Most of the genes were identified for the first time, providing potentially useful biomarkers for future assessments. For chronic exposures, no changes were detected in whole-body tissue; however, masculine reproductive organs showed changes in the expression of genes related to endocrine function (EcR, MAPR, AdipoR), epigenetic mechanisms (DNMTs), genotoxicity (PARP1), and stress responses (HSC70 4). For acute exposures, the expression of one epigenetic-related gene was altered for both whole-body tissues and male reproductive organs (Piwi2). Further changes were detected for whole-body tissues involved in detoxification (Metallothionein), stress (HSC70 4), and genotoxicity (PARP1) mechanisms. Acute exposure effects were also tested in whole-body tissues of juveniles, showing changes in the expression of Metallothionein and Piwi2. The molecular changes found in the analyzed earthworms indicate that exposure to BPA may have negative implications in their populations. Particularly interesting are the alterations related to epigenetic mechanisms, which suggest that future generations may be impacted. This study is the first to evaluate the molecular effects of BPA on soil organisms, and further assays will be necessary to better characterize

Bioavailability and bioaccumulation of heavy metals of several soils and sediments (from industrialized urban areas) for Eisenia fetida.

Science.gov (United States)

Coelho, C; Foret, C; Bazin, C; Leduc, L; Hammada, M; Inácio, M; Bedell, J P

2018-09-01

Soils and sediments are susceptible to anthropogenic contamination with Metallic Trace Elements (MTEs) and it can present some risks to ecosystems and human health. The levels of Cd, Cu, Fe, Ni, Pb and Zn were assessed in soils (C, G, K, L) from Estarreja (Portugal) and sediments from a stormwater basin in Lyon (DJG), a harbour (LDB) and a Rhône river site (TRS) (France). An ecotoxicological study was performed with Eisenia fetida (E. fetida) to infer about potential transfer risks to the soil invertebrates. To assess risks associated with MTEs contamination, it is important to know their total concentrations, fractionation and the potential available fractions. CaCl 2 , DTPA and NaOAc extractions were performed to assess the extractable and available MTEs fractions. The studied sediments were much more contaminated than the soils for all the MTEs analysed. The trace elements fraction linked with DTPA extraction shows higher values when compared with the NaOAc and the CaCl 2 pools. Low mortality effects were recorded in the tests with E. fetida. The MTEs levels in soils and sediments and the concentrations bioaccumulated in adult earthworms contributed to a reduction in the number of juveniles produced. E. fetida adults and juveniles accumulated ETMs as follows: Cd > Cu = Zn > Ni > Pb > Fe. Determined BAFs were mostly lower than 1 with some higher values for Cd, Cu and Zn. Calculated SET and ERITME indexes allowed to classify the samples from the most to the less toxic for E. fetida as: LDB > DJG > L > G > C > K > TRS. Despite this order of toxicity, the earthworms exposed to the sediment TRS presented the lowest reproduction rate. The combination of "chemical" measurements with the calculation of BAFs, but especially SET and ERITME indexes can be a useful tool in risk assessment. Copyright © 2018 Elsevier B.V. All rights reserved.

Earthworms (Eisenia fetida) demonstrate potential for use in soil bioremediation by increasing the degradation rates of heavy crude oil hydrocarbons.

Science.gov (United States)

Martinkosky, Luke; Barkley, Jaimie; Sabadell, Gabriel; Gough, Heidi; Davidson, Seana

2017-02-15

Crude oil contamination widely impacts soil as a result of release during oil and gas exploration and production activities. The success of bioremediation methods to meet remediation goals often depends on the composition of the crude oil, the soil, and microbial community. Earthworms may enhance bioremediation by mixing and aerating the soil, and exposing soil microorganisms to conditions in the earthworm gut that lead to increased activity. In this study, the common composting earthworm Eisenia fetida was tested for utility to improve remediation of oil-impacted soil. E. fetida survival in soil contaminated with two distinct crude oils was tested in an artificial (lab-mixed) sandy loam soil, and survival compared to that in the clean soil. Crude oil with a high fraction of light-weight hydrocarbons was more toxic to earthworms than the crude oil with a high proportion of heavy polyaromatic and aliphatic hydrocarbons. The heavier crude oil was added to soil to create a 30,000mg/kg crude oil impacted soil, and degradation in the presence of added earthworms and feed, feed alone, or no additions was monitored over time and compared. Earthworm feed was spread on top to test effectiveness of no mixing. TPH degradation rate for the earthworm treatments was ~90mg/day slowing by 200days to ~20mg/day, producing two phases of degradation. With feed alone, the rate was ~40mg/day, with signs of slowing after 500days. Both treatments reached the same end point concentrations, and exhibited faster degradation of aliphatic hydrocarbons C21, decreased. During these experiments, soils were moderately toxic during the first three months, then earthworms survived well, were active and reproduced with petroleum hydrocarbons present. This study demonstrated that earthworms accelerate bioremediation of crude oil in soils, including the degradation of the heaviest polyaromatic fractions. Copyright © 2016 Elsevier B.V. All rights reserved.

Reseña, La deuda pública interna. Reseña de los libros Un Estado a crédito. Deudas y configuración estatal de la Nueva Granada en la primera mitad del siglo XIX de Pilar López-Bejarano y Deuda pública interna en Colombia. Política, moneda y finanzas, 1840-1894 de Ángela Rojas.

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Salomón Kalmanovitz

2016-04-01

Full Text Available Estos dos libros marcan un quiebre en la historiografía de la construcción del Estado en Colombia. El de López-Bejarano busca desentrañar los desequilibrios en las cuentas fiscales del estado neogranadino, cuyos recursos “estuvieron sistemáticamente mediados por una serie de compromisos: vales, billetes y bonos que circularon respondiendo a intereses heterogéneos, públicos y privados” (p. 19. El de Ángela Rojas es un cuidado análisis de las cuentas fiscales, tanto de ingresos como de gastos, y ofrece una perspectiva macro de la deuda pública que los gobiernos de turno no pudieron o no quisieron pagar.

NCBI nr-aa BLAST: CBRC-XTRO-01-0669 [SEVENS

Lifescience Database Archive (English)

Full Text Available CBRC-XTRO-01-0669 ref|YP_995209.1| DNA internalization-related competence protein C...omEC/Rec2 [Verminephrobacter eiseniae EF01-2] gb|ABM56191.1| DNA internalization-related competence protein ComEC/Rec2 [Verminephrobacter eiseniae EF01-2] YP_995209.1 0.002 27% ...

The life-cycle of the compost worm (Oligochaeta) | Venter | African ...

African Journals Online (AJOL)

To determine the full potential of the compost worm Eisenia fetida as waste processor and as source of protein, the life-cycle of this species had to be studied thoroughly. The development, growth and reproduction of Eisenia fetida were studied on cattle manure under favourable conditions of moisture, temperature and ...

Diseño de un sistema alternativo para el tratamiento de aguas residuales urbanas por medio de la técnica de lombrifiltros utilizando la especie Eisenia foetida

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Jacipt Alexander Ramón

2015-06-01

Full Text Available La presente investigación tuvo como objetivo diseñar y construir un sistema sobre tratamiento de aguas residuales para la ciudad de Pamplona utilizando como base teórica el sistema de tratamiento Tohá creado por el Dr. Jose Tohá de la Universidad de Chile, proponiendo una serie de procesos que permiten el saneamiento de las aguas servidas después de su uso con fines domésticos, industriales o comerciales, para poder ser vertidas finalmente a cuerpos de agua naturales. Este proceso incluye un tratamiento con lombrifiltros (aserrín y Eisenia foetida, por donde las aguas residuales pasan seguidamente de una cama de carbón activado filtrando y una cama de grava de piedra, permitiendo oxigenar el agua a partir del goteo que existe entre las camas. Finalmente, el efluente sigue a una cama de piedra de rio de gran tamaño y a un decantador para realizar la toma de muestras. De esta manera se obtiene agua tratada con baja carga orgánica dentro de los parámetros que establece la Comisión de Regulación de Agua Potable y Saneamiento Básico CRA en Colombia.

Responses to silver nanoparticles and silver nitrate in a battery of biomarkers measured in coelomocytes and in target tissues of Eisenia fetida earthworms.

Science.gov (United States)

Patricia, Curieses Silvana; Nerea, García-Velasco; Erik, Urionabarrenetxea; Elena, Sáenz María; Eider, Bilbao; Darío, Di Marzio Walter; Manu, Soto

2017-07-01

The current use and development of applications with silver nanoparticles (Ag NPs) could lead to potential inputs of these NPs to soils. Consequently, it is crucial to understand the ecotoxicological risks posed by Ag NPs in the terrestrial compartment. In the present investigation, the effects produced by PVP-PEI coated Ag NPs were assessed in Eisenia fetida earthworms in comparison with the soluble form (AgNO 3 ). Earthworms were exposed for 1, 3 and 14 days to a range of sublethal concentrations of Ag (0, 0.05 and 50mg/kg) and at each exposure time, apart from mortality and weight loss of individuals, metallothionein (MT) protein concentration and catalase (CAT) activity were quantified in earthworm tissues. In addition, cellular and molecular level endpoints (cell viability, absolute and relative trophic indices and transcription levels of catalase-cat- and metallothionein-mt-) were measured in coelomocytes extruded from exposed earthworms. Despite the lack of effects in traditional endpoints (mortality and weight loss), Ag NPs and AgNO 3 posed changes at lower levels of biological complexity (biochemical, cellular and molecular levels). Both Ag forms induced similar changes in the metal detoxification mechanism (MT, mt) and in the antioxidant response system (CAT, cat) of E. fetida. In contrast, Ag form dependant cytotoxicity and subpopulation ratio alterations (eleocytes/amoebocytes) were recorded in extruded coelomocytes. Complementarily, the use of coelomocytes to assess molecular level endpoints represented a relevant alternative for development of non-invasive biomarkers. Copyright © 2017 Elsevier Inc. All rights reserved.

Caracterización de la composición en proteína, lípidos, energía y perfiles de ácidos grasos en huevos de tilapia roja (Oreochromis spp.

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Rubén Darío Valbuena-Villarreal

2013-06-01

Full Text Available Luego de considerar el significado de las tilapias en el escenario piscícola nacional, la posibilidad de mejorar los índices de producción es un imperativo técnico y, dentro de estos, focalizar esfuerzos experimentales en la semilla resulta ser una opción estratégica. Con el fin de establecer un referente de base se utilizaron las puestas individuales de 50 hembras de tilapia roja (Oreochromis spp. para determinar los contenidos en los huevos de proteína (% MS, extracto etéreo (% MS, energía (cal g-1 y los perfiles de ácidos grasos (%, con algunas relaciones de interés analítico. Salvo ciertas variaciones menores, los valores encontrados se ajustan a los registros disponibles para la especie en diferentes estudios, lo que sugiere que las dietas ofrecidas a los reproductores experimentales contienen los mínimos necesarios para asegurar una provisión de nutrientes y energía suficientes para los embriones y las larvas en formación. Se analiza que, como punto de partida, esta serie de datos tiene utilidad para el desarrollo de trabajos destinados a mejorar los estándares de producción de alevinos, considerando que las variaciones en la composición, que pueden ser determinadas sobre los huevos, se pueden utilizar y tienen validez como un indicativo directo de su calidad.

Alternativas orgánicas para disminuir la aplicación de nitrógeno en nogal pecanero

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Juan Manuel Soto Parra

2016-01-01

Full Text Available Introducción: El nitrógeno es el nutriente más demandado por los árboles de nogal pecanero, por lo que es considerado un factor limitante para su desarrollo, producción y calidad de la nuez. Sin embargo, ante el aumento en el precio de los fertilizantes y sus efectos adversos en el ambiente, actualmente es más evidente la necesidad de aplicar nutrientes de manera sustentable. Una alternativa para disminuir la aplicación de fertilizantes nitrogenados inorgánicos en nogal pecanero, es complementarlo con composta, humus de lombriz, lixiviado de lombriz y microorganismos potenciadores de la fertilidad del suelo, como son las micorrizas, entre otros. Por lo que el objetivo del estudio fue reducir la aplicación de nitrógeno inorgánico al suelo mediante la adición de enmiendas orgánicas y microorganismos potenciadores de la fertilidad del suelo sin afectar producción ni calidad. Método: El estudio se realizó en el municipio de Aldama, Chihuahua, durante los ciclos 2010-2012 en árboles ‘Western Schley’, se utilizó una estructura Taguchi L16 para cuatro factores y cuatro niveles para cada factor: nitrógeno 330, 220, 110 y 0 kg ha-1; composta 0, 2.5, 5.0, 7.5 t ha-1; humus de lombriz (OptiHumusMR 0, 1.5, 3.0, 4.5 t ha-1 y micorrizas (Sehumic-VamMR 0, 7.5, 15.0, 22.5 g pulg-1 de tronco. Se determinó el contenido de materia orgánica y nitratos en el suelo, producción y calidad de la nuez, los cuales se analizaron por superficie de respuesta. Resultados: A pesar de que para producir 2.9 t ha-1 se logró reducir la aplicación de nitrógeno, este nivel no permitió obtener una buena calidad de la nuez ni alcanzar un contenido adecuado de materia orgánica y nitratos en suelo. Para un rendimiento de 2.9 t ha-1 con 153 nueces kg-1 y 59.4% de nuez comestible, 1.06% de materia orgánica y 183 ppm de nitratos en suelo se requirieron 305 kg de N, 6600 kg de composta, 3500 kg de humus de lombriz y 18 g de micorrizas por pulg-1 de tronco

Algunos problemas relacionados con las ilustraciones en las traducciones de una obra clásica de la literatura infantil y juvenil: el caso de Caperucita Roja en España

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Hanna Martens

2012-09-01

Full Text Available http://dx.doi.org/10.5007/2175-795X.2012v30n3p817 En una edición ilustrada de una obra literaria los elementos verbales se combinany completan con el aspecto visual del volumen para transmitir su mensaje al lector.Al traducir la obra a otro idioma, se intenta, en la medida de lo posible, respetar elequilibrio establecido entre la información proporcionada en el texto y en las imágenes.Si se trata de una obra clásica, sin embargo, que se sigue editando a lo largo de variossiglos, la situación suele complicarse, ya que, como es habitual, no sólo se realizannuevas traducciones, sino que también van cambiando las ilustraciones. Este es el casode las traducciones españolas del cuento de “Le Petit Chaperon Rouge” (CaperucitaRoja de Perrault. Las ilustraciones han tenido una importancia innegable en larecepción de la obra, creando cierto ambiente y completando las caracterizaciones delos personajes. Así, algunas ilustraciones se han mostrado particularmente influyentes,no sólo dejando su huella en los ilustradores de las generaciones posteriores, sinollegando a introducir también modificaciones en las traducciones del propio cuento.Además, es tal el poder de las imágenes que llegan a manipular el contenido de la obra,destacando ciertos elementos en detrimento de otros o defendiendo abiertamentecierta ideología. A veces, incluso, pueden llegar a producirse incongruencias entre eltexto y las imágenes que lo acompañan.

Nuevas especies, nombres nuevamente utilizados y nuevas distribuciones en los helechos arborescentes (Filicales: Cyatheaceae para el Neotrópico

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Alexander Fco. Rojas - Alvarado

2001-06-01

Full Text Available Dos especies nuevas son descritas para el Neotrópico, cuatro especies anteriormente sinonimizadas son reconocidas, y se amplián la distribución de cuatro especies y una variedad. Las nuevas especies son: Cyathea grayumii A. Rojas y C. panamensis A. Rojas. Cyathea alfonsiana L. D. Gómez, C. holdridgeana Nisman et L. D. Gómez, C. onusta H. Christ y C. squarrosa (Rosenst. Domin son nuevamente aceptadas, y Cnemidaria coclena Stolze, Cyathea andina (Karst. Domin, C. caracasana var. meridensis (H. Karst. R. M. Tryon, C. macrosora y C. pseudonanna (L. D. Gómez Lellinger son ampliadas en su distribución.Two new species are described for the Neotropic, four species are newly accepted, and four species and one variety are reported. The new species are: Cyathea grayumii A. Rojas and C. panamensis A. Rojas. Cyathea alfonsiana L. D. Gómez, C. holdridgeana Nisman & L. D. Gómez, C. onusta H. Christ and C. squarrosa (Rosenst. Domin are recognized; Cnemidaria coclena Stolze, Cyathea andina (H. Karst. Domin, C. caracasana var. meridensis (H. Karst. R. M. Tryon, C. macrosora (Baker Domin and C. pseudonanna (L. D. Gómez Lellinger are reported from Costa Rica and Panama.

Effects of ultraviolet B (UVB) on different varieties of quinoa. I. Effects on morphology under controlled conditions

International Nuclear Information System (INIS)

Pérez, M.L.; Prado, F.E.; González, J.A.

2015-01-01

Effects of ultraviolet UV-B (UVB) on different varieties of quinoa. I. Effects on morphology under controlled conditions. The effects of UV-B radiation (RUV-B) on growth parameters: plant height (H), stem diameter (SD), length x wide (LW), leaf number (LN), specific leaf area (SLA) and specific leaf mass (SLM) of five quinoa varieties are described. RUV-B effects were different according to analyzed variety and parameter. The H was increased in CICA (P ≤ 0,04) and Robura (P ≤ 0,02) varieties, while SD was increased in CICA (P ≤ 0,0002) and Faro Roja (P ≤ 0,017) varieties. The LW changed significantly in CICA (P ≤ 0,05) variety only. The LN showed positive changes in all quinoa varieties exposed to RUV-B. Highest changes were found in Faro Roja (P ≤ 0,003), CICA (P ≤ 0,003) and Ratuqui (P ≤ 0,015) varieties. The SLM positively changed in Faro Roja, Kancolla and Robura varieties (P ≤ 0,05). The CICA variety showed significant increases in all evaluated parameters, followed by Faro Roja and Robura varieties. Less parameter changes occurred in Kancolla and Ratuqui varieties. The observed changes were discussed in terms of adaptive evolution. (authors) [es

Caracterización de tilapia roja (Oreochromis sp. con marcadores moleculares RAPD Characterization of the red tilapia Oreochromis sp. through molecular markers RAPD

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Julieta Torres Jaramillo

2010-04-01

Full Text Available Se utilizó la técnica RAPD (amplificación al azar de ADN polimórfico para el estudio de la diversidad genética de Oreochromis sp. (tilapia roja en cinco piscícolas del Valle del Cauca (Colombia y en la determinación del nivel de introgresión de las especies parentales Oreochromis mosambicus, O. niloticus y O. aureus. Se evaluaron 25 cebadores, ocho fueron polimórficos y se obtuvieron 109 bandas. Los valores de heterocigosidad esperada (0.196 a 0.256 y la estructura genética (Gst = 0.22 para Oreochromis sp. indicaron un elevado grado de polimorfismo y alta estructuración genética. Estos resultados fueron consistente con el Fst = 0.268 (P Random amplified polymorphic DNA (RAPD markers were used to study genetic diversity on red Tilapia (Oreochromis sp. species collected from five fish farms located in the Valle del Cauca, Colombia and to determine the level of introgression from three parental species O. mosambicus, O. niloticus and O. aureus into local Oreochromis populations. from the 25 RAPD primers evaluated, eight were polymorphic and 109 banding patterns were observed, any of them were specific. The expected levels of heterozygosis (0.1964 to 0.2561 and genetic structure (Gst = 0.22 funded for Oreochrosmis sp. indicate high grade of polymorphism and genetic structuring. This results were observed following the analysis of molecular variance [AMOVA] (Fst = 0.268 (P <0.0001 and Multiple correspondence analysis (Gst = 0.040. The values of genetic similarity, the analysis of group, the analysis of multiple correspondence and the level of introgression, indicated that the differences in the introgression levels(P=0.0001 were significant. The low level of observed genetic differentiation among populations, could be the result of fish with the same genetic origin, whereas the high variation within populations can be displayed by handling practices and the pressure of selection to favor commercial phenotypes. The level of introgression

Terapia de choque

DEFF Research Database (Denmark)

Agustin, Oscar Garcia

2012-01-01

No hay alternativa, nos dicen. Ya no hay líneas rojas. Sólo un poco más de terapia de choque para aumentar el desorden bipolar entre los que tienen y los que no.......No hay alternativa, nos dicen. Ya no hay líneas rojas. Sólo un poco más de terapia de choque para aumentar el desorden bipolar entre los que tienen y los que no....

La marea roja causada por el dinoflagelado Alexandrium tamarense en la costa Pacífica colombiana (2001

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Ingrid García-Hansen

2004-09-01

Full Text Available El 26 de marzo de 2001 se registró por primera vez en las aguas del Pacífico colombiano, en el área de la Ensenada de Tumaco, la aparición de una marea roja producida por la especie Alexandrium tamarense con valores que superaron las 7.5 x 10(6 céls l-1 , la marea se mantuvo hacia la región oceánica, derivando en sentido sur-norte por efecto de las corrientes, hasta ser vista por ultima vez cerca a la Isla Gorgona, casi tres semanas después. Un año mas tarde, en marzo de 2002, una segunda proliferación, conformada por la misma especie, se reportó cerca de Cabo Corrientes con una concentración de 1.6 x 10(6 céls l -1 . Ambos casos estuvieron relacionados con la presencia de bajas temperaturas en la superficie del mar y no estuvieron asociadas con fenómenos de intoxicación y mortalidadFrom April 26th to May 15th 2001, a large algae bloom was observed off Tumaco Bay on the Pacific coast of Colombia. This was the first harmful algae bloom (HAB reported in the region, and reached Gorgona Island, about 120 km north. Ayear later, starting March 2002, an offshore HAB developed from Cabo Corrientes North to Solano Bay. The typical abundance during the blooms reached 7.5 x 10(6 cells l-1 for the 2001 event and 1.6 x 10(6 cells l-1 for the 2002 event. During both events, low temperature and high salinity were recorded. Typical measurements in the area are 27-27.5°C and 30-31.5 psu. Values observed during the two events were 24-24.6°C and 33-34 psu; 3°C below normal and more than 2.5 psu above average values. These conditions are indicative of local upwelling processes at the time of the events. On both occasions, cells corresponding to the Alexandrium catenella/fundeyense/tamarense complex represented 99-100% of the biomass. It was difficult to differentiate the cells from A. catenella, but the presence of short chains of only 4 cells (single cells represented most of the biomass was suggestive of A. tamarense. Shape, dimensions, and

cinco sustratos orgánicos

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Lolita Durán

2007-01-01

Full Text Available Se tomaron separadamente volúmenes de 0,03 m3 de desechos domésticos, estiércol de vacuno, residuo de banano, follaje de ornamentales y broza de café. Estos materiales fueron colocados en cajas individuales e inoculados con 600 lombrices del tipo “roja californiana” (Eisenia foetida. Luego de 3 meses, la variabilidad en las propiedades de los vermicompostes fue alta. Tanto el tratamiento con residuo de banano como el doméstico presentaron los mayores contenidos de K (7 y 3%, respectivamente; el de doméstico fue el mayor en N (3,1%. Ambos presentaron los mayores valores de pH y contenido de sales. El valor de P mayor se encontró en el tratamiento de estiércol, con un 2%. El doméstico mostró el mayor porcentaje de espacio aéreo y el menor porcentaje de retención de agua, lo cual se relacionó con una mayor proporción de macroporos. Todos los materiales tuvieron densidades diferentes entre sí. La disminución del volumen inicial al final fue 80% para doméstico y ornamental, 83% para banano, 53% para estiércol y 67% para broza. La mayor abundancia de microorganismos se encontró en los vermicompostes provenientes de residuos de banano y doméstico, con las poblaciones mayores de actinomicetes y hongos. En conclusión, las características finales de los vermicompostes pueden ser muy diferentes y están determinadas en buena parte por la naturaleza de las fuentes orgánicas utilizadas para su elaboración.

Registro de un ejemplar de Trachemys scripta elegans (Wied 1839) en la provincia de Jujuy, Argentina

OpenAIRE

Quiroga, María Fernanda; González Baffa Trasci, Noelia; Akmentins, Mauricio

2015-01-01

La tortuga de orejas rojas (Trachemys scripta elegans) es originaria del centro este de los Estados Unidos y noreste de México (Ernst, 1990), pero debido al comercio internacional de animales silvestres se ha convertido en un invasor biológico a nivel global. En Argentina, Trachemys scripta elegans ha sido reportada para dos localidades en la provincia de Buenos Aires y en ambos casos se trató del registro de individuos aislados, por lo que se desconoce si la tortuga de orejas rojas ha lograd...

Accumulation and Sublethal Effects of Triclosan and its Transformation Product Methyl-triclosan in the Earthworm Eisenia andrei Exposed to Environmental Concentrations in an Artificial Soil.

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Chevillot, Fanny; Guyot, Mélanie; Desrosiers, Mélanie; Cadoret, Nicole; Veilleux, Éloïse; Cabana, Hubert; Bellenger, Jean-Philippe

2018-04-18

Municipal biosolids are increasingly used as a low-cost fertilizer in agricultural soil. Biosolids are contaminated by low concentrations (ng g -1 dw range) of a large variety of organic contaminants, such as triclosan (TCS). The effect of exposure to low concentrations of organic contaminants on soil biota remains largely undocumented. We evaluated the sublethal effects of TCS on the earthworm Eisenia andrei using an artificial soil amended with a nominal concentration of TCS of 50 ng g -1 dry weight soil. Using a 56-d reproduction test, we monitored the effect of TCS exposure on adult earthworm survival, growth, and reproduction. The bioaccumulation of TCS in earthworm tissue (adults and juveniles) and degradation of TCS were monitored. The genotoxicity of TCS was evaluated using a comet assay (DNA damage) on adult earthworm coelomocytes. Exposure to a low concentration of TCS had no significant effects on adult earthworm survival and DNA damage, but significantly stimulated growth (P increase in the number of cocoons and juveniles, and a decrease in the mean dry weight of juveniles. The bioaccumulation of TCS in earthworms was moderate (bioaccumulation factor ∼ 2). In biosolid-borne trials, the bioaccumulation of methyl-triclosan in earthworm tissues was higher than the parent compound TCS. We conclude that exposure to low concentrations of TCS in artificial soil can significantly affect the growth and reproductive performance of earthworms (i.e., E. andrei). More research is required with natural soils to assess TCS bioavailability for earthworms. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Toxicokinetics of Zn and Cd in the earthworm Eisenia andrei exposed to metal-contaminated soils under different combinations of air temperature and soil moisture content.

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González-Alcaraz, M Nazaret; Loureiro, Susana; van Gestel, Cornelis A M

2018-04-01

This study evaluated how different combinations of air temperature (20 °C and 25 °C) and soil moisture content (50% and 30% of the soil water holding capacity, WHC), reflecting realistic climate change scenarios, affect the bioaccumulation kinetics of Zn and Cd in the earthworm Eisenia andrei. Earthworms were exposed for 21 d to two metal-contaminated soils (uptake phase), followed by 21 d incubation in non-contaminated soil (elimination phase). Body Zn and Cd concentrations were checked in time and metal uptake (k 1 ) and elimination (k 2 ) rate constants determined; metal bioaccumulation factor (BAF) was calculated as k 1 /k 2 . Earthworms showed extremely fast uptake and elimination of Zn, regardless of the exposure level. Climate conditions had no major impacts on the bioaccumulation kinetics of Zn, although a tendency towards lower k 1 and k 2 values was observed at 25 °C + 30% WHC. Earthworm Cd concentrations gradually increased with time upon exposure to metal-contaminated soils, especially at 50% WHC, and remained constant or slowly decreased following transfer to non-contaminated soil. Different combinations of air temperature and soil moisture content changed the bioaccumulation kinetics of Cd, leading to higher k 1 and k 2 values for earthworms incubated at 25 °C + 50% WHC and slower Cd kinetics at 25 °C + 30% WHC. This resulted in greater BAFs for Cd at warmer and drier environments which could imply higher toxicity risks but also of transfer of Cd within the food chain under the current global warming perspective. Copyright © 2018 Elsevier Ltd. All rights reserved.

Effects of benzo[a]pyrene on growth, the antioxidant system, and DNA damage in earthworms (Eisenia fetida) in 2 different soil types under laboratory conditions.

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Duan, Xiaochen; Xu, Li; Song, Jing; Jiao, Jiaguo; Liu, Manqiang; Hu, Feng; Li, Huixin

2015-02-01

The aims of the present study were to compare the toxic effects of benzo[a]pyrene (BaP) and to screen for rapid and sensitive biomarkers that can be used to assess the environmental risks of BaP in earthworms in different natural soil types. The authors exposed Eisenia fetida to 2 types of soil (red soil and fluvo-aquic soil) spiked with different concentrations (0 mg kg(-1), 1 mg kg(-1), 10 mg kg(-1), 100 mg kg(-1), and 500 mg kg(-1)) of BaP for 7 d or 14 d. Benzo[a]pyrene-induced weight variation altered the activities of antioxidant enzymes (superoxide dismutase [SOD]; catalase [CAT]; and guaiacol peroxidase [POD]) and changed the content of malondialdehyde (MDA). In addition, using the comet assay, the authors determined the DNA damage in earthworms. The results revealed that the comet assay was suitable for evaluating the genotoxicity of BaP in the soil, even at the lowest examined concentration. The MDA content was the least sensitive indicator of BaP toxicity. A 3-way analysis of variance (ANOVA) was used to determine whether the soil type, exposure concentration, and duration affected the BaP toxicity. The antioxidant enzyme activities and the MDA content were shown to be significantly correlated with the exposure concentration. The percentage of weight variation (p earthworms is a simple and efficient means of assessing BaP genotoxicity in a terrestrial environment, and the effects of the soil type and exposure time on the other parameters that were investigated in E. fetida, which were used as responsive biomarkers, should be considered. © 2014 SETAC.

Scarlet Macaw, Ara macao, (Psittaciformes: Psittacidae diet in Central Pacific Costa Rica

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Christopher Vaughan

2006-09-01

Full Text Available From 1993 to 1997, we observed Scarlet Macaw (Ara macao feeding behavior in Central Pacific Costa Rica. Feeding data acquired in this study were not collected systematically, but opportunistically whenever macaws were observed feeding. To supplement feeding observations, we conducted interviews with local residents. Scarlet Macaws fed on seeds, fruits, leaves, flowers and/or bark of 43 plant species. Various plant parts eaten by macaws from several tree species contain secondary compounds toxic to humans, and additional species included in their diet are nonnative, introduced for agricultural purposes. Important macaw feeding tree species are Ceiba pentandra, Schizolobium parahybum, and Hura crepitans; these species are also crucial to this macaw population because of nest cavities they provide. The results of this study contribute to the conservation of Scarlet Macaws in Central Pacific Costa Rica through promoting protection of individual trees, and through local elementary school reforestation programs focusing on tree species that macaws use for feeding and/or nesting. Scarlet Macaw conservation is extremely important, as numerous population pressures have caused significant declines in macaw numbers in Costa Rica. Rev. Biol. Trop. 54 (3: 919-926. Epub 2006 Sept. 29.Entre 1993-97, observamos el comportamiento de alimentación de la lapa roja (Ara macao en el Pacifico Central de Costa Rica. La lapa roja se alimentó de semillas, frutas, hojas, flores y corteza de 43 especies de plantas. Varias partes de las plantas comidas por las lapas contienen compuestos secundarios tóxicos al ser humano, y especies adicionales incluidas en la dieta son exóticas; introducidas por razones agrícolas, forestales o estéticas. Especies de árboles importantes como alimento de la lapa roja incluyen: Ceiba pentandra, Schizolobium parahybum, y Hura crepitans; también son criticas para la población de la lapa roja debido a proveen cavidades para anidaci

Taxonomic revision of the species of Colletes Latreille, 1802 (Hymenoptera: Colletidae: Colletinae) found in Chile.

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Ferrari, Rafael R

2017-12-13

A taxonomic revision of Colletes Latreille species with known geographic distribution in Chile is presented. In addition to the traditional morphological approach to taxonomy, DNA barcoding was employed to facilitate sexual association and cryptic species recognition. I provide diagnoses, synonymies, geographic and floral records, and a fully-illustrated key for 31 recognized species, 23 of them previously described: C. alocochila Moure, C. atacamensis Janvier, C. atripes Smith, C. bicolor Smith, C. chusmiza Rojas & Toro, C. cognatus Spinola, C. cyanescens (Haliday), C. cyaniventris Spinola n. stat., C. flaminii Moure, C. fulvipes Spinola, C. gilvus Vachal, C. guanta Rojas & Toro, C. longiceps Friese, C. lucens Vachal, C. mastochila Moure, C. murinus Friese, C. musculus Friese, C. nigritulus Friese, C. patagonicus Schrottky, C. quelu Rojas & Toro, C. rutilans Vachal, C. sulcatus Vachal, and C. vicugnensis Rojas & Toro. In addition, eight new species are described: C. arthuri n. sp., C. coquimbensis n. sp., C. flavipilosus n. sp., C. kuhlmanni n. sp., C. nigropilosus n. sp., C. simulatus n. sp., C. toroi n. sp., and C. ventricarinatus n. sp. Lectotypes for the following species are designated: Andrena cyanescens, Colletes bicolor, C. campoi Herbst, C. chubutensis Cockerell, C. gilvus, C. lucens, C. patagonicus, C. rufosignatus Cockerell, and C. viridans Vachal. Colletes seminitidus Spinola and C. viridans are both proposed as junior synonyms of C. cyanescens, and C. araucariae Friese is considered a junior synonym of C. sulcatus. Colletes cyaniventris n. stat. is resurrected from synonymy.

Selección masal por peso y coloración en tilapia roja Mass selection by weight and coloration in red tilapia

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María Alejandra Ruiz Peña

2009-04-01

Full Text Available Para evaluar la efectividad de la selección masal por color y peso se analizaron dos generaciones (G1 y G2 de alevinos de tilapia roja Oreochromis sp. De 40.000 larvas a la sexta semana se eliminaron los alevinos manchados y blancos y a las 14 semanas se separaron por sexos. Se midió el peso (g, la longitud total (cm, la altura (cm y el ancho (cm de 150 individuos a las 6, 14 y 24 semanas de edad. En la semana 24 se escogieron 150 machos y 450 hembras con las coloraciones deseables y mayor peso. La selección resultó efectiva para coloración en G2, puesto que la proporción de individuos rojos se incrementó en 15% con respecto a los testigos. Entre generaciones (G1 y G2 el efecto de la selección fue positivo, debido a que la proporción de rojos se incrementó de 64% a 84% y se redujo la de manchados de 31% a 13%. En ambas generaciones los machos fueron significativamente más pesados que las hembras. Se encontraron notables diferencias entre generaciones para peso y talla, puesto que los selectos superaron al control en 27% y 8% (G1 y en 22% y 11% (G2 para el peso y la talla, respectivamente.In order to evaluate the effectiveness of mass selection by color and weight, two generations (G1 y G2 of red tilapia Oreochromis sp. were analyzed. 40000 larvae were used, at the sixth week fries with black spots and white were eliminated, and at week 14, the fishes were separated by sex. The weight (g, length, height and width (cm were measured in a sample of 150 individuals at 6, 14 and 24 weeks. At week 24, the best 150 males and 450 females with desirable colorations and higher weight were chosen. The selection was effective for coloration in G2, because proportion of red individuals increased in 15% compared to the control. The effect of the selection between generations (G1 y G2 was positive because proportion of red fries increased from 64% to 84%, and the spotted ones were reduced from 31% to 13%. In both generations males were significantly

[New species, newly used names and new ranges of tree ferns (Filicales: Cyatheaceae) in the Neotropics].

Science.gov (United States)

Rojas-Alvarado, A F

2001-06-01

Two new species are described for the Neotropics, four species are newly accepted, and four species and one variety are reported. The new species are: Cyathea grayumii A. Rojas and C. panamensis A. Rojas. Cyathea alfonsiana L. D. Gómez, C. holdridgeana Nisman & L. D. Gómez, C. onusta H. Christ and C. squarrosa (Rosenst.) Domin are recognized; Cnemidaria coclena Stolze, Cyathea andina (H. Karst.) Domin, C. caracasana var. meridensis (H. Karst.) R. M. Tryon, C. macrosora (Baker) Domin and C. pseudonanna (L. D. Gómez) Lellinger are reported from Costa Rica and Panama.

La Remolacha

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García Paredes Antonio

1942-08-01

Full Text Available La remolacha es una planta exótica, herbácea y bisanual del género BETA, familia de las QUENOPO HEACEAS. Se cultiva la remolacha por sus usos en la economía doméstica y sobre todo su importancia en la industria azucarera. Se conocen cuatro variedades de remolacha: blanca, roja, amarilla y vetada, todas tienen el mismo sabor azucarado, pero para comer se prefiere la roja por su bello color de amaranto que se lo comunica a los líquidos en que se prepara.

La remolacha

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García Paredes, Antonio

2012-01-01

La remolacha es una planta exótica, herbácea y bisanual del género BETA, familia de las QUENOPO HEACEAS. Se cultiva la remolacha por sus usos en la economía doméstica y sobre todo su importancia en la industria azucarera. Se conocen cuatro variedades de remolacha: blanca, roja, amarilla y vetada, todas tienen el mismo sabor azucarado, pero para comer se prefiere la roja por su bello color de amaranto que se lo comunica a los líquidos en que se prepara.

Actividad antiparasitaria de una decocción de Mentha piperita Linn

OpenAIRE

de la Paz Naranjo, José; Maceira Cubiles, María Acelia; Corral Salvadó, Aida; González Campos, Carlos

2006-01-01

Con el objetivo de comprobar si la Mentha piperita Linn posee efecto antiparasitario, se valoró una decocción de las hojas de esta planta. El modelo biológico utilizado fue la lombriz terrestre del género rojo California y las dosis empleadas fueron 0,475; 0,950 y 1,900 g/dL. Se formaron además un grupo control negativo (agua destilada) y un grupo control positivo (solución de piperazina al 2,0 %). La evaluación, en placa Petri, se realizó de forma continua durante un período de 8 h. La varia...

Eritroblastosis

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Javier Isaza

1948-07-01

Full Text Available Es un síndrome que se caracteriza por la persistencia o revivimiento de centros hematopoyéticos embrionarios en la médula ósea roja, en el hígado y en el bazo, focos que se forman como reacción de defensa a la destrucción exagerada de eritrocitos provocada por una reacción feto-materna. Con proliferación intensa de la serie roja embrionaria y producción de eritrocitos nucleados, acompañada de hemolisis, anemia, ictericia, hepatomegalia y esplenomegalia de intensidad variables, y reacción mieloide.

Immunocytochemical electron microscopic study and western blot analysis of paramyosin in different invertebrate muscle cell types of the fruit fly Drosophila melanogaster, the earthworm Eisenia foetida, and the snail Helix aspersa.

Science.gov (United States)

Royuela, M; García-Anchuelo, R; Arenas, M I; Cervera, M; Fraile, B; Paniagua, R

1996-04-01

The presence and distribution pattern of paramyosin have been examined in different invertebrate muscle cell types by means of Western blot analysis and electron microscopy immunogold labelling. The muscles studied were: transversely striated muscle with continuous Z lines (flight muscle from Drosophila melanogaster), transversely striated muscle with discontinuous Z lines (heart muscle from the snail Helix aspersa), obliquely striated body wall muscle from the earthworm Eisenia foetida, and smooth muscles (retractor muscle from the snail and pseudoheart outer muscular layer from the earthworm). Paramyosin-like immunoreactivity was localized in thick filaments of all muscles studied. Immunogold particle density was similar along the whole thick filament length in insect flight muscle but it predominated in filament tips of fusiform thick filaments in both snail heart and earthworm body wall musculature when these filaments were observed in longitudinal sections. In obliquely sectioned thick filaments, immunolabelling was more abundant at the sites where filaments disappeared from the section. These results agree with the notion that paramyosin extended along the whole filament length, but that it can only be immunolabelled when it is not covered by myosin. In all muscles examined, immunolabelling density was lower in cross-sectioned myofilaments than in longitudinally sectioned myofilaments. This suggests that paramyosin does not form a continuous filament. The results of a semiquantitative analysis of paramyosin-like immunoreactivity indicated that it was more abundant in striated than in smooth muscles, and that, within striated muscles, transversely striated muscles contain more paramyosin than obliquely striated muscles.

SUPEROXIDE DISMUTASE FROM THE EARTHWORM (EISENIA FOETIDA)%蚯蚓体内超氧化物歧化酶分析

Institute of Scientific and Technical Information of China (English)

刘堰; 张平波

1999-01-01

蚯蚓体内SOD含量甚高,35℃饲养的蚯蚓其SOD比活最高,因此,纯化前将蚯蚓在35℃养殖4周以上.采用硫酸铵分级沉淀和柱层析的方法,从蚯蚓体内分离得到纯的铜锌超氧化物歧化酶.每100g组织得到SOD制品总活力为17,190 U,比活7995 U/mg,回收率为35%.该酶呈淡蓝绿色,最大紫外吸收波长为270nm.该酶分子量为33,000,亚基分子量为16,500.该酶亚基含156个氨基酸残基,不含酪氨酸.N-末端为丙氨酸,等电聚焦为三条谱带,等电点分别为5.30 、5.59和6.22.%The superoxide dismutase activity (units/mg protein) was determined for earthworm (Eisenia foetida) acclimated from 10℃to 35℃at 5 degree intervals. Worms acclimated at 35℃gave a value of SOD units significantly higher than those acclimated at other temperatures . So the worms were acclimated for four weeks at 35℃prior to purification. Cupro-zinc superoxide dismutase extracted from the cell of earthworm had been purified by ammonium sulphate precipitation and special column chromatography . The purified enzyme obtained from 100 grams of tissue possessed a total activity of 17 190 units with specific activity of 7 995 units per mg protein .The yield was 35%. The enzyme isolated by this procedure showed pale blue-green color . It exhibited one absorption maximum in the ultraviolet at 270 nm .The molecular weight of the purified enzyme was 33 000 daltons as determined by gel filtration on Sephadex G-100 , and that of the enzyme subunits was 16 500 daltons as estimated with sodium dodecyl sulphate-polyacrylamide gel electrophoresis .The enzyme subunits consisted of 156 amino acid residues , in which tyrosine was absent . The N-terminal of the enzyme was alanine as assayed by dansyl chloride . Three bands of the enzyme were seen in isoelectrofocusing. They focused at pH5.30, 5.59 and 6.22 , respectively .

Tres miradas sobre paisaje, identidad regional y cultura folclórica en Santiago del Estero

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Judith Farberman

2010-06-01

Full Text Available Este artículo explora las relaciones entre paisaje, identidad y cultura popular en algunas obras de Ricardo Rojas, Bernardo Canal Feijóo y Orestes Di Lullo sobre Santiago del Estero. Consideramos que Canal Feijóo y Di Lullo regresaron en sus trabajos de las décadas de 1930 y 1940 a tres cuestiones tratadas por Rojas en El país de la selva (1907, aunque en un contexto ya irremediablemente cambiado de destrucción del bosque chaqueño. La primera de ellas consiste en la búsqueda de los orígenes de la cultura folclórica santiagueña en el período colonial. En ausencia de flujos consistentes de inmigración europea, los autores se remitieron a la conquista y a los mestizajes biológicos y culturales posteriores como punto de partida para la formación de una cultura local que ambos estimaban aún vigorosa y original. La segunda es la identidad entre paisaje y cultura folclórica. Di Lullo y Canal compartían con Rojas la idea de que la imaginación popular nacía de la naturaleza o, mejor dicho, de la sensibilidad de sujetos íntimamente consustanciados con ella. En consecuencia, la destrucción del paisaje sólo podía conducir a la del folclore, al que encontraban ya irremediablemente degradado. En tercer lugar, se hallaba la elevación del folclore santiagueño a marca de origen, a sello identitario. El componente indígena, hispano o mestizo se encontraba presente, en diferentes medidas, en aquel patrimonio ancestral que, como Rojas lo había hecho antes, Canal y Di Lullo describieron e interpretaron a la luz de sus personales lecturas de la historia santiagueña.This article explores the relationship between landscape, identity and popular culture of Santiago del Estero in the works of Ricardo Rojas, Bernardo Canal Feijóo and Orestes Di Lullo. In my opinion, Di Lullo and Canal Feijóo revisited El país de la selva (1907 in some of their works, especially in those written in the 30's and 40's. Three issues are analyzed here. The first

Lethal and sub-lethal evaluation of Indigo Carmine dye and byproducts after TiO2 photocatalysis in the immune system of Eisenia andrei earthworms.

Science.gov (United States)

Genázio Pereira, Patrícia Christina; Reimão, Roberta Valoura; Pavesi, Thelma; Saggioro, Enrico Mendes; Moreira, Josino Costa; Veríssimo Correia, Fábio

2017-09-01

The Indigo carmine (IC) dye has been widely used in textile industries, even though it has been considered toxic for rats, pigs and humans. Owing to its toxicity, wastes containing this compound should be treated to minimize or eliminate their toxic effects on the biota. As an alternative to wastewater treatment, advanced oxidative processes (AOPs) have been highlighted due to their high capacity to destruct organic molecules. In this context, this study aimed to evaluate Indigo Carmine toxicity to soil organisms using the earthworm Eisenia andrei as a model-organism and also verify the efficiency of AOP in reducing its toxicity to these organisms. To this end, lethal (mortality) and sub-lethal (loss or gain of biomass, reproduction, behavior, morphological changes and immune system cells) effects caused by this substance and its degradation products in these annelids were evaluated. Morphological changes were observed even in organisms exposed to low concentrations, while mortality was the major effect observed in individuals exposed to high levels of indigo carmine dye. The organisms exposed to the IC during the contact test showed mortality after 72h of exposure (LC 50 = 75.79mgcm - 2 ), while those exposed to photoproducts showed mortality after 48h (LC 50 = 243min). In the chronic study, the organisms displayed a mortality rate of 14%, while those exposed to the photoproduct reached up to 32.7%. A negative influence of the dye on the reproduction rate was observed, while by-products affected juvenile survival. A loss of viability and alterations in the cellular proportion was verified during the chronic test. However, the compounds did not alter the behavior of the annelids in the leak test (RL ranged from 20% to 30%). Although photocatalysis has been presented as an alternative technology for the treatment of waste containing the indigo carmine dye, this process produced byproducts even more toxic than the original compounds to E. andrei. Copyright © 2017

Avaliação do Potencial Tóxico de Latossolos e Chernossolos Acrescidos de Lodo de Esgoto Utilizando Bioensaios com Oligoquetas da Espécie Eisenia andrei.

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Ricardo Gonçalves Cesar

2008-08-01

Full Text Available Sewage Sludge (SEL has been used in agricultural activities to improve physical and chemical properties of soils. However, thiscompound also contains very high levels of heavy metals and pathogenic microorganisms, which can cause serious negative effects onbiota and human health. This paper aims to assess potential toxicity of latosols and chernosols amended with SEL, using Eisenia andreiearthworms. Acute toxicity and behavioral tests were performed according to ASMT (2004 and ISO (2002 procedures, respectively.Heavy metals determination was made by Atomic Absorption, and mineralogical characterization by Ray-X Difratometry. Biomass losswas evaluated considering average individual weight. Mercury, lead, zinc and cooper determination in SEL in natura and soils amendedwith SEL revealed values higher than that recommended by Brazilian legislation. SEL addition to the soils caused no mortality to theorganisms, while SEL in natura provoked lethal effects on 100% of the earthworms. Latosol in natura demonstrated higher mortalityin comparison to chernosol in natura Behavioral tests indicated that 98,3% and 96,43% of the organisms avoided SEL in natura andlatosol with SEL, respectively. However, only 31% of the organisms avoided chernosol with SEL, suggesting a lower level of toxicity incomparison to latosol. Possibly the natural properties of the studied soils (fertility, organic matter content, clay mineralogy, etc. playedan important role in the avoidance behavior of the organisms. Probably expansive clays presence in chernosol stimulates ions adsorptionand their concentrations decreasing in soil solution; consequently contaminants bioavailability also tends to decrease. Biomass loss resultsrevealed that the SEL addition decreased the weight loss of the tested organisms, and biomass losses were higher for latosol than forchernosol. In conclusion, it is expected that these results can be used in human health and ecological risk assessment, as well as

Lo que son mujeres en los escenarios

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González Cañal, Rafael

2007-06-01

Full Text Available In this article I review the publishing and stage trajectory of one of Rojas Zorrilla’s most significant plays: Lo que son mujeres. As well as collecting a list of the different editions, I emphasize the 1822 version by the Mexican Manuel Eduardo de Gorostiza, which started a new renaissance period for this play on stage. I also analyze the 1899 one-act version by Emilio Bobadilla. Finally, I include all the different productions between the 17th and the 19th centuries, and address the little interest aroused in the 20th century–suffice to say that it has not been staged even during these early years of the 21st century when the centenary of Rojas Zorrilla is being celebrated.En este artículo se hace un repaso detallado de la fortuna editorial y escénica de una de las obras más significativas de Rojas Zorrilla: Lo que son mujeres. Aparte de recoger las diferentes ediciones de la misma, se hace hincapié en la refundición del mexicano Manuel Eduardo de Gorostiza, estrenada en 1822, que abrió un período de cierta recuperación de este texto dramático en los escenarios. Se analiza también la refundición que llevó a cabo en un solo acto Emilio Bobadilla en 1899. Finalmente, se registran todas las representaciones conocidas de la obra desde el siglo XVII hasta el siglo XIX y se constata el poco interés que ha despertado en los escenarios del siglo XX. Incluso en los últimos años, en los que los montajes de obras de Rojas comienzan a ser más frecuentes, esta obra no ha sido todavía llevada a escena.

Evaluación agronómica de líneas avanzadas de fríjol voluble Phaseolus vulgaris L. en Paipa, Boyacá

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Diana Katherine Ríos

2014-06-01

Full Text Available En Boyacá la oferta de germoplasma de frijol mejorado es baja. El objetivo de este estudio fue evaluar el comportamiento agronómico de 18 líneas avanzadas volubles de frijol, procedentes del CIAT. Se utilizó el diseño bloques completos al azar, con tres repeticiones, incluyendo Bola Roja, Bolívar y Cargamanto como testigos. Se evaluó días a floración, vigor de crecimiento, días a madurez, incidencia de enfermedades, número de vainas/planta, número de granos/vaina, peso de 100 granos y rendimiento. Los genotipos MAC27, MAC13, MBC26, MBC28 y MBC37 no se diferenciaron del Cargamanto pero sí mostraron ser más precoces que Bola Roja y Bolívar. En días a madurez MAC27 y MBC39 fueron más tempranos que los testigos. Todos los genotipos se comportaron como resistentes a enfermedades, excepto MBC30 que resultó susceptible a Uromyces phaseoli y MBC33 a Phoma exigua. En número de vainas por planta, número de granos por vaina, peso de 100 semillas y rendimiento se destacaron MAC27 y LAS399, sin diferencia significativa frente a Cargamanto pero si superando a Bola Roja y Bolívar. Pese al efecto de las heladas en la fase final del ciclo, LAS399, MAC27 y Cargamanto produjeron rendimientos de 1300, 1038 y 950 kg/ha, respectivamente, mostrando las ventajas de su precocidad, en comparación a Bola Roja y Bolívar que son tardíos. Se concluye que LAS399 y MAC27, por su alta precocidad y resistencia a patógenos, son las líneas más promisorias para continuar la evaluación respecto a la posibilidad de ampliar la oferta de germoplasma de frijol voluble en Boyacá.

Características antioxidantes, fisicoquímicas y microbiológicas de jugo fermentado y sin fermentar de tres variedades de pitahaya (Hylocereus spp

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Carlos Enrique Ochoa-Velasco

2012-01-01

Full Text Available Al jugo de pitahaya de tres variedades (roja, rosa y blanca se le evaluó el pH, sólidos solubles totales, acidez titulable, color, compuestos fenólicos, capacidad antioxidante, mientras que al jugo de pitahaya fermentado con Saccharomyces cerevisiae se le evaluaron además el número de bacterias mesófilas aerobias, mohos y levaduras y contenido alcohólico. Los sólidos solubles totales y pH disminuyeron en el jugo fermentado en todas las variedades de pitahaya. El contenido de compuestos fenólicos fue mayor en el jugo de pitahaya roja, seguido de la rosa y la blanca con valores promedios de 45,3, 32,1y 24,6 mg de ácido gálico/100 mL de jugo, respectivamente. Por ende, la capacidad antioxidante fue superior en el jugo de pitahaya roja (160,84 mg de Trolox/100 mL de jugo. La fermentación del jugo de pitahaya incrementó el contenido de compuestos fenólicos (variando de 45,31 a 51,38 mg de ácido gálico/100 mL de jugo, en pitahaya roja, aunque disminuyó la capacidad antioxidante (variando de 124,51 a 82,96 mg de Trolox/100 mL de jugo, en pitahaya rosa. El contenido de alcohol de los jugos fermentados fue entre 2,9 y 3,9 % (v/v. Al final de la fermentación la carga máxima de mohos y levaduras (1580 UFC/mL la presentó el jugo fermentado de pitahaya blanca. Sin embargo, después de la pasteurización el crecimiento microbiano en todos los tratamientos fue menor a 10 UFC/mL. La elaboración de subproductos como el jugo fermentado es una alternativa viable para la comercialización de productos derivados de la pitahaya.

Pogostemon cablin (Blanco) Benth

African Journals Online (AJOL)

SAM

2014-06-04

Jun 4, 2014 ... Bheemagani1, Prem Kumar1, Venkatesh K1 and Anupalli Roja Rani1. 1Department ... Human infections caused by microorganisms and fungi are rampant ... gram negative bacteria (Escherichia coli, Enterobacter aerogenes).

Efeitos Tóxicos de Arsênio em Eisenia Andrei em Exposição a Solos do Entorno de Minerações de Ouro

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Rodrigo Henrique Alves

2015-06-01

Full Text Available Os produtos das atividades de mineração têm grande relevância econômica no Estado de Minas Gerais, Brasil. No entanto, seus inúmeros danos à biota, incluindo a fauna edáfica, resultam na necessidade de recuperação e biomonitoramento dessas áreas e do seu entorno. Considerando a importância ecológica e o potencial bioindicador das minhocas, o objetivo deste estudo foi avaliar a toxicidade de arsênio presente em solos do entorno de minerações de ouro, nas bacias dos rios Doce e São Francisco, a oligoquetas da espécie Eisenia andrei Bouché. Amostras de solos dessas áreas foram caracterizadas em relação às suas propriedades físicas e químicas, incluindo os metais Fe, Al, Cu, Ni, Zn e Mn e do metaloide As; com elas, foram conduzidos bioensaios, que avaliaram a sobrevivência, reprodução e variação da biomassa dos organismos. Também foram realizados testes de sensibilidade dessa espécie ao As adicionado a solo artificial tropical e em solo natural. Houve evidente efeito tóxico sobre a reprodução dessa espécie, que esteve altamente correlacionado com as concentrações de As nas amostras (r = -0,80. Amostras de solos naturais de locais avaliados nas duas áreas apresentaram concentrações de As acima do limite estipulado pela Resolução Conama nº 420 de 2009, chegando a 2.388,2 mg kg-1. A CL50 do As em solo artificial tropical (207,4 mg kg-1 foi seis vezes menor que a do solo natural (1.248,1 mg kg-1. A menor biodisponibilidade do As nos solos naturais foi atribuída a sua associação com o minério de Fe presente nas amostras. Concluiu-se que os solos do entorno de áreas de mineração causaram toxicidade crônica a E. andrei, em termos de sua reprodução, o que demonstrou o potencial de danos dessas atividades sobre a fauna edáfica dessas áreas. O estudo também forneceu informações ecotoxicológicas que podem ser úteis em avaliações de risco ambiental em solos tropicais.

Occupational and Environmental Risks from Mancozeb in Aviation ...

African Journals Online (AJOL)

1Colegio de Postgraduados Campus Tabasco, Periferico Carlos A. Molina S/N Carr. .... Base system [14], the predominant soils correspond to Fluvisols, Gleysols ..... Ficha de datos de seguridad R. D. River Fungicida Rojas. Mancozeb [River ...

Hey! A Fire Ant Stung Me!

Science.gov (United States)

... Videos for Educators Search English Español Hey! A Fire Ant Stung Me! KidsHealth / For Kids / Hey! A ... Me picó una roja o colorada! What's a Fire Ant? There are many different types of fire ...

Lorena reeglid / Lorena Rojas ; interv. Kaisa Karu

Index Scriptorium Estoniae

Rojas, Lorena

2007-01-01

Mehhiko telenovelade staar, kes mängib ka sel hooajal TV3s jooksvas seriaalis "Teine võimalus" ("El cuerpo del deseo"), valiti ajak. People poolt 2006.a. maailma 100 ilusama naise hulka. Sama : Teleleht nr. 3, lk. 38 : ill

Reconfirmation of antimicrobial activity in the coelomic fluid of the ...

Indian Academy of Sciences (India)

Unknown

723. Keywords. Antimicrobial activity; column chromatography; earthworm; Eisenia fetida andrei; Tetrazolium salt ... fair resistance against E. coli, P. aeruginosa and Arthrobacter sp., respectively. [Pan W, Liu X, Ge F ... 2.2 Test bacteria species.

NCBI nr-aa BLAST: CBRC-XTRO-01-0560 [SEVENS

Lifescience Database Archive (English)

Full Text Available CBRC-XTRO-01-0560 ref|YP_995556.1| binding-protein-dependent transport systems inne...r membrane component [Verminephrobacter eiseniae EF01-2] gb|ABM56538.1| binding-protein-dependent transport systems

NCBI nr-aa BLAST: CBRC-CPOR-01-1994 [SEVENS

Lifescience Database Archive (English)

Full Text Available CBRC-CPOR-01-1994 ref|YP_995620.1| binding-protein-dependent transport systems inne...r membrane component [Verminephrobacter eiseniae EF01-2] gb|ABM56602.1| binding-protein-dependent transport systems

NCBI nr-aa BLAST: CBRC-XTRO-01-0287 [SEVENS

Lifescience Database Archive (English)

Full Text Available CBRC-XTRO-01-0287 ref|YP_997917.1| binding-protein-dependent transport systems inne...r membrane component [Verminephrobacter eiseniae EF01-2] gb|ABM58899.1| binding-protein-dependent transport systems

NCBI nr-aa BLAST: CBRC-XTRO-01-0887 [SEVENS

Lifescience Database Archive (English)

Full Text Available CBRC-XTRO-01-0887 ref|YP_995619.1| binding-protein-dependent transport systems inne...r membrane component [Verminephrobacter eiseniae EF01-2] gb|ABM56601.1| binding-protein-dependent transport systems

NCBI nr-aa BLAST: CBRC-XTRO-01-0534 [SEVENS

Lifescience Database Archive (English)

Full Text Available CBRC-XTRO-01-0534 ref|YP_994945.1| binding-protein-dependent transport systems inne...r membrane component [Verminephrobacter eiseniae EF01-2] gb|ABM55927.1| binding-protein-dependent transport systems

Luis José de Tejada y Guzmán : espejo de peregrinos penitentes

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Darío Achury Valenzuela

1979-03-01

Full Text Available Graciela Maturo, docta escritora argentina, ha escrito un estudio documentado y erudito bajo el rubro de Luis de Tejada y su peregrino místico, editado por el Instituto de Literatura Argentina "Ricardo Rojas", de la Universidad de Buenos Aires.

Sociólogo sentipensante

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Renán Vega Cantor

2013-09-01

Full Text Available Antología. Orlando Fals Borda. José María Rojas Guerra (Prefacio. Universidad Nacional de Colombia, Vicerrectoría Académica, Instituto de Estudios Políticos y Relaciones Internacionales, Bogotá, 2010, 387 págs.

10.-21. VI toimub Ruhnu saarel keskkondliku skulptuuri workshop

Index Scriptorium Estoniae

2004-01-01

Läti ja Eesti kolmeetapilise koostööprojekti eesmärk on rikastada ja väärtustada Roja, Ruhnu ja Salacgriva keskkonda. Osalejad: Vilnis Titans, Girts Burvis, Maris Sarshuns Lätist, Eneken Maripuu, Rait Pärg, Leena Kuutma, Peeter Pajo Eestist

The advertisement call of Centrolene notostictum (Anura, Centrolenidae) with a new record of geographic distribution in Tolima, Colombia.

Science.gov (United States)

Viuche-Lozano, Andres; Enciso-Calle, Maria Paula; Bernal, Manuel Hernando

2018-02-05

Centrolene notostictum Ruiz-Carranza Lynch,1991 is a glassfrog known from the Cordillera Oriental of Colombia-in the Departments of Boyacá, Cundinamarca, Norte de Santander and Santander (Frost 2017)-and the Venezuelan versant of the Sierra de Perijá (Rojas et al. 2012) from 1600 to 2440 masl. Although males have been found calling on the upper surface of the leaves of ferns and Heliconeaceae on the margins of a small fast-flowing stream (Rojas et al. 2012), no quantitative description of the advertisement call of C. notostictum is currently available. During fieldwork in the Galilea Natural Forest (on the western slope of Cordillera Oriental in the municipality of Villarrica, department of Tolima, Colombia) in 2016 and 2017, we found three vocalizing males of C. notostictum. Herein, we report the first geographic record of this species for the department of Tolima and describe its previously unknown advertisement call.

Composición química de cúmulos globulares galácticos: metalicidades derivadas a partir de fotometría de Washington

Science.gov (United States)

Torres, M. C.; Clariá, J. J.

Se presentan temperaturas efectivas y abundancias de 183 gigantes rojas en 7 cúmulos globulares galácticos, derivadas a partir de fotometría de Washington realizada en Cerro Tololo. Las metalicidades provienen de una calibración que tiene en cuenta la disminución de sensibilidad de algunos índices con la temperatura. Los valores medios de [Fe/H] obtenidos oscilan entre -1.2 y -2.15, con errores típicos del orden de 0.2 salvo un par de excepciones. Teniendo en cuenta que el índice (C-T1) es significativamente más sensible a la metalicidad que (V-I), se presenta una calibración de abundancia basada en el color (C-T1)o de la rama gigante roja para una determinada magnitud absoluta M(T1). Las abundancias derivadas confirman en general la escala de Zinn (1985, ApJ, 293, 424).

Effects of azoxystrobin, chlorothalonil and ethoprophos on the reproduction of three terrestrial invertebrates using a natural Mediterranean soil

NARCIS (Netherlands)

Leitao, S.; Cerejeira, J.; Brink, van den P.J.; Sousa, J.P.

2014-01-01

The potential terrestrial toxicity of three pesticides, azoxystrobin, chlorothalonil, and ethoprophos was evaluated using reproduction ecotoxicological tests with different non-target species: the collembolan Folsomia candida, the earthworm Eisenia andrei, and the enchytraeid Enchytraeus crypticus.

EVALUACIÓN DE ALTERNATIVAS PARA CLIMATIZACIÓN DE ESTANQUES CON ENERGÍA SOLAR PARA CULTIVO DE TILAPIA ROJA (Oreochromis sp, LOCALIZADOS EN LA ZONA FRÍA DEL VALLE DEL CAUCA, COLOMBIA EVALUATION OF ALTERNATIVES FOR AIR CONDITIONING OF PONDS WITH SOLAR ENERGY FOR CULTIVATION OF RED TILAPIA (Oreochromis sp, LOCATED IN THE COLD AREA OF THE CAUCA VALLEY, COLOMBIA

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Francisco Javier Borja Gallardo

2006-06-01

Full Text Available La investigación fue desarrollada para que la tilapia roja (Oreochromis sp, especie de pez mÁs producida en Colombia, pueda ser explotada en lugares donde las condiciones óptimas para su normal desarrollo no son las adecuadas, como son las regiones altas con temperaturas por debajo de los 24 °C, aplicando metodologías relacionadas con el aprovechamiento de la energía solar. Las técnicas evaluadas fueron: climatización por medio de colectores solares, climatización por medio de manta térmica, climatización por medio de mangueras térmicas y climatización por medio de resistencias eléctricas. Se analizaron factores como funcionalidad, mantenimiento, limitaciones organizativas, espaciales, y costos.The research was developed so that the red tilapia (Oreochromis sp, fish species that more taken place in Colombia can be exploited in regions where the good conditions for its normal development are not the appropriate ones as in the high regions with temperatures below the 24 °C, applying methodologies related with the use of the solar energy. The evaluated methodologies were: air conditioning by means of solar collectors, air conditioning by means of thermal blanket, air conditioning by means of thermal hoses and air conditioning by means of electric resistances. Factors like functionality, maintenance, organizational and space limitations, and costs were analized.

65 Comparative Studies of Some Polypores Using High ...

African Journals Online (AJOL)

Administrator

Comparative Studies of Some Polypores Using High Performance Liquid. Chromatography. Ofodile L. N., Attah L. E. Agbaje Williams and Simmonds, M. S. J ... Chromatography characteristics of their secondary metabolites. ..... Roja, G. and Rao, P. S. (1998) Biotechnology investigation in medicinal plants for the product.

Comparative Genomics of Symbiotic Bacteria in Earthworm Nephridia

DEFF Research Database (Denmark)

Kjeldsen, Kasper Urup; Pinel, Nicolas; Lund, Marie Braad

The excretory and osmoregulatory organs (nephridia) of lumbricid earthworms are densely colonized by extracellular bacterial symbionts belonging to the newly established betaproteobacterial genus Verminephrobacter. The nephridial symbiont of the earthworm Eisenia fetida was subjected to full geno...

Vermiremediation of Soils Contaminated with Mixture of Petroleum ...

African Journals Online (AJOL)

ADOWIE PERE

order to clean-up soil contaminated with gasoline, diesel and spent engine oil using an earthworm - Eisenia ... hydrocarbon (TPH) level every 24 hours over a period of 120 hours using gas chromatography. ... adverse effects. The need to ...

Determination of multi-walled carbon nanotube bioaccumulation in earthworms measured by a microwave-based detection technique

Science.gov (United States)

Reliable quantification techniques for carbon nanotubes (CNTs) are limited. In this study, a new procedure was developed for quantifying multi-walled carbon nanotubes (MWNTs) in earthworms (Eisenia fetida) based on freeze drying and microwave-induced heating. Specifically, earthw...

El vuelo zumbón de la libélula

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Fernando González Cajiao

1992-01-01

Full Text Available Delírium titerensis. Tres obras de la Libélula Dorada: el dulce encanto de la isla Acracic, Los espíritus lúdicos y Ese chivo es puro cuento (ilustraciones de Carlos Rojas. Arango Editores, Bogotá, 1991, 129 págs.

Herbicide Glyphosate Impact to Earthworm (E. fetida

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Greta Dajoraitė

2016-10-01

Full Text Available Glyphosate is a broad spectrum weed resistant herbicide. Glyphosate may pose negative impact on land ecosystems because of wide broad usage and hydrofilic characteristic. The aim of this study was to investigate negative effects of glyphosate on soil invertebrate organisms (earthworm Eisenia fetida. The duration of experiment was 8 weeks. The range of the test concentrations of glyphosate were: 0,1, 1, 5, 10, 20 mg/kg. To investigate the glyphosate impact on earthworm Eisenia fetida the following endpoints were measured: survival, reproduction and weight. The exposure to 20 mg/kg glyphosate has led to the 100% mortality of earthworms. Glyphosate has led to decreased E. fetida reproduction, the cocoons were observed only in the lowest concentration (0,1 mg/kg. In general: long-term glyphosate toxicity to earthworms (E. fetida may be significant.

Nuevos hallazgos en la cueva de Ekain (Gipuzkoa, País Vasco

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Jesús ALTUNA

2009-08-01

Full Text Available RESUMEN: Se da cuenta de nuevos hallazgos en la cueva de Ekain, a raíz de la realización de la réplica llevada a cabo de dicha cueva. En dos de los casos, probables vulva y bóvido, se aprovechan accidentes de la roca, tales como bordes rocosos, volúmenes, grietas y hoyuelos, cosa repetida en numerosas figuras de esta cueva. La vulva es un hoyo fusiforme relleno de pintura roja, igual a la del caballo contiguo. El posible bóvido es un reborde rocoso, que puede sugerir el vientre de un animal al que se han señalado con trazos negros el arranque de la pata anterior y zonas de la cabeza y dorso. Por ello comienza el trabajo indicando estos aprovechamientos en figuras ya conocidas anteriormente. En los demás casos se trata de líneas, puntos y manchas negras y rojas. Así un conjunto de ellas están en la galería Auntzei cerca del salmón y de las pequeñas cabras que se encuentran en esta galería, tanto a un lado como al otro de la misma. Son en total 8 manchas rojas, 1 negra, 5 puntos rojos, 1 negro y 5 trazos negros. En la zona de Erdialde, zona central de la cueva, hay más manchas rojas así como líneas y puntos negros, que suman un total de 4 manchas rojas, 2 puntos rojos, 4 puntos negros y 7 trazos o líneas negras.ABSTRACT: We describe the new findings in Ekain cave due to the replica that has been done of this cave. In two cases, “vulva and bovid”, the relief of the wall has been used such as edges of the rock, volumes, cracks or small holes, and this has been repited in many other paintings of this cave. The “vulva” is a fusiform hole with red painting in it, the same red painting as the horse nearby. The posible “bovid” is a ridge in the rock that may suggest the abdomen of an animal where the “legs and arms” have been removed as well as the head and the “torax”. This is the reason why the paper starts describing the use of the cave relief in other known cases in this cave. The rest of the new findings are

On ‘war task’ and ‘peace work’. The Dutch East Indies Red Cross between the colonial wars and the Second World War

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van Bergen, Leo

2014-06-01

Full Text Available After the wars against Atjeh (1873-1907 finished, Dutch rule over the Dutch East India was total. The Dutch East Indies Red Cross (DEIRC awaited a new task, preparing to give aid in the case of a foreign invasion. The problem was that the end of the wars against the autochthonous “rebellions” also meant the end of Red Cross visibility and in the minds of many, the end to Red Cross urgency. Aid in wars against a real opponent is from a point of public relations much more important than the preparation of aid against an unknown opponent. “Work in times of peace” had to be the answer to this problem, but this work was only in name, and not de facto different from preparation of aid in times of war. Through “peace work” the DEIRC prepared itself for the war-task. To be able to fulfil the war-task the Red Cross had to have enough doctors, nurses, and stretcher-bearers. Visible peace-work had to provide for that. It enlarged the Red Cross’ popularity and trained its volunteers. However, at the beginning of 1942, when the Japanese invaded the Dutch East Indies, it turned out to be too little, too late.Tras el fin de las guerras contra Aceh (1873-1907, el dominio holandés sobre la India Oriental Holandesa fue total. La Cruz Roja de las Indias Orientales Holandesas (DEIRC quedaba a la espera de una nueva tarea: prepararse para proporcionar ayuda en caso de una invasión extranjera. El problema era que el final de las guerras contra las “rebeliones” autóctonas también significaba el fin de la visibilidad de la Cruz Roja y, a juicio de muchos, el final de la perentoriedad de la Cruz Roja. El socorro en guerras contra un adversario real era desde el punto de vista de las relaciones públicas mucho más importante que los preparativos de socorro contra un enemigo desconocido. El “trabajo en tiempo de paz” debía ser la respuesta a este problema, si bien resultó diferente solo de palabra, no de hecho, en relación a los preparativos

Onderzoek naar de geschiktheid van de potwormsoorten Enchytraeus albidus en E. crypticus (Oligochaeta, Annelida) in bodemecotoxicologisch onderzoek

NARCIS (Netherlands)

Dirven van Breemen EM; Baerselman R; Notenboom J; ECO

1994-01-01

This report describes a research on the use of enchytraeids in soil ecotoxicology. Actually many soil ecotoxicological research is restricted to the earthworm Eisenia andrei/fetida limiting possibilities for generalisation and ecologically relevant test results. This study on enchytraeids consits of

Onderzoek naar de geschiktheid van de potwormsoorten Enchytraeus albidus en E. crypticus (Oligochaeta, Annelida) in bodemecotoxicologisch onderzoek

NARCIS (Netherlands)

Dirven-van Breemen EM; Baerselman R; Notenboom J; ECO

1994-01-01

This report describes a research on the use of enchytraeids in soil ecotoxicology. Actually many soil ecotoxicological research is restricted to the earthworm Eisenia andrei/fetida limiting possibilities for generalisation and ecologically relevant test results. This study on enchytraeids consits

IMPLICANCIA ECOLOGICA DE LA COALESCENCIA EN EL SUBGENERO TYLECODIUM CLOROPHYTA

OpenAIRE

GONZALEZ VASQUEZ, ALEJANDRA PAOLA; GONZALEZ VASQUEZ, ALEJANDRA PAOLA

2007-01-01

Basados en características morfológicas y ecológicas, se ha propuesto que la especie Codium dimorphum (subgénero Tylecodium) es un alga verde capaz de coalescer, proceso que hasta el momento permanece como exclusivo de las algas rojas. En este contexto, e 223p.

EL PROYECTO DE LA OLIMPIADA POPULAR DE BARCELONA (1936, ENTRE COMUNISMO INTERNACIONAL Y REPUBLICANISMO REGIONAL

Directory of Open Access Journals (Sweden)

André Gounot

2005-01-01

Full Text Available Los archivos del KOMINTERN en el Centro Ruso de Conservación y Estudio de los Documentos sobre la Historia Contemporánea (CRCRHC de Moscú, recientemente abiertos para el estudio científico, nos ofrecen fuentes documentales fundamentales para entender el desarrollo de la Internacional Deportiva Roja (IDR como una de las organizaciones auxiliares del citado KOMINTERN. Las referidas fuentes documentales han aportado nuevas informaciones y datos sobre la creación en España en 1931, con el establecimiento de la 2ª República, de la Federación Cultural y Deportiva Obrera (FCDO, organización que en 1934 se adhiere a la Internacional Deportiva Roja (IDR, siendo el embrión del nacimiento del Comité Catalán pro Deporte Popular que presentaría el proyecto de la Olimpiada Popular de Barcelona de 1936 como alternativa a la Olimpiada de Berlín de 1936 de Hitler. Por otra parte la Internacional Deportiva Roja (IDR creó a finales de 1935 el Comité Internacional para la defensa de la Idea Olímpica, cuyo objetivo era coordinar los movimientos de oposición a los Juegos de Berlín. También la propia Federación Cultural y Deportiva Obrera (FDCO fundó en 1936 el Comité Español de Defensa del Espíritu Olímpico con este mismo fin. La Olimpiada Popular de Barcelona de 1936 permitió entre otras cosas expresar la nueva alianza entre Comunismo y Olimpismo, construida con discursos contradictorios apoyándose en el universalismo, la paz y la igualdad de razas.

Crónica roja: ni blanco ni negro.

Directory of Open Access Journals (Sweden)

Orlando Pérez

2015-01-01

Full Text Available Frente a la interrogante de ¿Qué hacer ante la sentencia que los reos reciben de los medios de comunicación antes de que el juez instaure el proceso? y ¿Cómo enfrentar responsablemente las diversas presiones que tiene el cronista de sucesos? el autor plantea algunas posibilidades: más que los hechos, los procesos; antes de juzgar primero investigar; reportar el fenómeno no el delito, entre otras. Relieva el valor de la Ética en todo trabajo de un reportero de la sección judicial.

Functional and community-level soil microbial responses to zinc addition may depend on test system biocomplexity.

NARCIS (Netherlands)

Sverdrup, L.E.; Linjordet, R.; Stomman, G.; Hagen, S.B.; van Gestel, C.A.M.; Frostegard, A.; Sorheim, R.

2006-01-01

The effect of zinc on soil nitrification and composition of the microbial community in soil was investigated using a full factorial experiment with five zinc concentrations and four levels of biological complexity (microbes only, microbes and earthworms (Eisenia fetida), microbes and Italian

Comparison of landfarming amendments to improve bioremediation of petroleum hydrocarbons in Niger Delta soils

NARCIS (Netherlands)

Brown, David M; Okoro, Samson; van Gils, Juami; van Spanning, Rob; Bonte, Matthijs; Hutchings, Tony; Linden, Olof; Egbuche, Uzoamaka; Bruun, Kim Bye; Smith, Jonathan W N

2017-01-01

Large scale landfarming experiments, using an extensive range of treatments, were conducted in the Niger-Delta, Nigeria to study the degradation of oil in contaminated soils. In this work the effect of nutrient addition, biosurfactant, Eisenia fetida (earthworm) enzyme extract, bulking and sorption

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African Journals Online (AJOL)

RAGHAVENDRA

n with chickpea as green manure had higher N, P and K in. 43.75 g per pot. The micro ..... conservation which is utilized for better root penetration and crop growth. .... Vermicomposting of coffee pulp using the earthworm. Eisenia fetida: effects ...

Irradiation studies of non-human biota

International Nuclear Information System (INIS)

Shetty, N.J.; Chaubey, R.C.; Harini, B.P.; Jha, S.K.

2014-01-01

In this study the effect of gamma radiation on the Drosophila melanogaster, Eisenia fetida, Apis mellifera and Aedes aegypti were studied for its effect on factors like fecundity, hatchability, adult emergence, sex ratio, longevity, dose response curve and estimation of heat shock protein

Nanosilver pathophysiology in earthworms: Transcriptional profiling of secretory proteins and the implication for the protein corona

DEFF Research Database (Denmark)

Hayashi, Yuya; Miclaus, Teodora; Engelmann, Péter

2016-01-01

Previously we have identified lysenin as a key protein constituent of the secretome from Eisenia fetida coelomocytes and revealed its critical importance in priming interactions between the cells and the protein corona around nanosilver. As alterations of the protein environment can directly affe...

Color image segmentation using perceptual spaces through applets ...

African Journals Online (AJOL)

Color image segmentation using perceptual spaces through applets for determining and preventing diseases in chili peppers. JL González-Pérez, MC Espino-Gudiño, J Gudiño-Bazaldúa, JL Rojas-Rentería, V Rodríguez-Hernández, VM Castaño ...

Phenolic Content and Antioxidant Capacity in Algal Food Products

Directory of Open Access Journals (Sweden)

Ludmila Machu

2015-01-01

Full Text Available The study objective was to investigate total phenolic content using Folin-Ciocalteu’s method, to assess nine phenols by HPLC, to determine antioxidant capacity of the water soluble compounds (ACW by a photochemiluminescence method, and to calculate the correlation coefficients in commercial algal food products from brown (Laminaria japonica, Eisenia bicyclis, Hizikia fusiformis, Undaria pinnatifida and red (Porphyra tenera, Palmaria palmata seaweed, green freshwater algae (Chlorella pyrenoidosa, and cyanobacteria (Spirulina platensis. HPLC analysis showed that the most abundant phenolic compound was epicatechin. From spectrophotometry and ACW determination it was evident that brown seaweed Eisenia bicyclis was the sample with the highest phenolic and ACW values (193 mg·g−1 GAE; 7.53 µmol AA·g−1, respectively. A linear relationship existed between ACW and phenolic contents (r = 0.99. Some algal products seem to be promising functional foods rich in polyphenols.

Laboratory experiments examining inducible defense show variable responses of temperate brown and red macroalgae Experimentos de laboratorio para examinar las defensas inducibles muestran respuestas variables en macroalgas pardas y rojas de ambientes templados

Directory of Open Access Journals (Sweden)

EVA ROTHÄUSLER

2005-12-01

Full Text Available Macroalgae can defend themselves against generalist and specialist herbivores via morphological and/or chemical traits. Herein we examined the defensive responses (via relative palatability of two brown (Lessonia nigrescens, Glossophora kunthii and two red algae (Grateloupia doryphora, Chondracanthus chamissoi from the northern-central coast of Chile against selected generalist meso-herbivores. Two laboratory experiments were conducted to investigate whether (i algae can respond generally to grazing pressure of meso-herbivores (amphipods, isopods and juvenile sea urchins and whether (ii these algal responses were inducible. In order to examine palatability and thus effectiveness of responses, feeding assays were run after each experiment using fresh algal pieces and artificial agar-based food. Lessonia nigrescens responded to amphipods but not to sea urchins, and G. kunthii showed inducible response against one species of amphipods. Grateloupia doryphora did not respond against any of the tested grazers, whereas C. chamissoi responded against one species of amphipods and the tested isopod. Our results indicate variable responses of macroalgae against selected generalist meso-herbivores and evidence of an inducible defense in the brown alga G. kunthii.Muchas macroalgas poseen la capacidad de defenderse contra herbívoros generalistas y especialistas utilizando defensas químicas y/o morfológicas. En este trabajo se examinó la respuesta de la palatabilidad ante meso-herbívoros generalistas de dos algas pardas (Lessonia nigrescens, Glossophora kunthii y dos algas rojas (Grateloupia doryphora, Chondracanthus chamissoi de la costa Norte de Chile. Se realizaron dos experimentos de laboratorio para investigar si: (i las algas pueden responder al pastoreo realizado por meso-herbivoros generalistas (anfípodos, isópodos y erizos juveniles y (ii si la respuesta de estas algas es inducible. Para examinar la palatabilidad y de esta forma la efectividad

Species composition of a soil invertebrate multi-species test system determines the level of ecotoxicity

DEFF Research Database (Denmark)

Sechi, Valentina; D'Annibale, Alessandra; Maraldo, Kristine

2014-01-01

A soil multi-species, SMS, experimental test system consisting of the natural microbial community, five collembolan species and a predatory mite along with either Enchytraeus crypticus or the earthworm Eisenia fetida were exposed to α-cypermethrin. A comparison of the performance of these two typ...

Regulatory ecotoxicity testing of nanomaterials – proposed modifications of OECD test guidelines based on laboratory experience with silver and titanium dioxide nanoparticles

DEFF Research Database (Denmark)

Hund-Rinke, Kerstin; Baun, Anders; Cupi, Denisa

2016-01-01

of the sediment-living worm Lumbriculus variegatus (TG 225), activity of soil microflora (TGs 216, 217), and reproduction of the invertebrates (Enchytraeus crypticus, Eisenia fetida, TGs 220, 222). Additionally, test descriptions for two further test systems (root elongation of plants in hydroponic culture; test...

Developing Quantum Chemical and Polyparameter Models for Predicting Environmentally Significant Parameters for New Munition Compounds

Science.gov (United States)

2017-05-31

in fish , plants, and soil invertebrates have been used to build the models. In addition the BCFs for a soil invertebrate (oligochaete Eisenia......Streit B, Nagel R. Tubifex tubifex as a link in food chain transfer of hexachlorobenzene from contaminated sediment to fish . Hydrobiologia

Can commonly measurable traits explain differences in metal accumulation and toxicity in earthworm species?

NARCIS (Netherlands)

Qiu, H.; Peijnenburg, W.J.G.M.; van Gestel, C.A.M.; Vijver, M.G.

2014-01-01

There is no clear consensus in the literature on the metal accumulation pattern and sensitivity of different earthworm species. In the present study, accumulation and toxicity of Cu, Cd, Ni, and Zn in the earthworms Lumbricus rubellus (epigeic), Aporrectodea longa (anecic), and Eisenia fetida

Soil acidification increases metal extractability and bioavailability in old orchard soils of Northeast Jiaodong Peninsula in China

NARCIS (Netherlands)

Li, Lianzhen; Wu, Huifeng; van Gestel, C.A.M.; Peijnenburg, W.J.G.M.; Allen, Herbert E.

2014-01-01

The bioavailability of Cu, Zn, Pb and Cd from field-aged orchard soils in a certified fruit plantation area of the Northeast Jiaodong Peninsula in China was assessed using bioassays with earthworms (Eisenia fetida) and chemical assays. Soil acidity increased with increasing fruit cultivation periods

Bulletin of Materials Science | Indian Academy of Sciences

Indian Academy of Sciences (India)

Centro de Investigación en Nanociencia y Nanotecnología (NANOTEC), Facultad Regional Córdoba, Universidad Tecnológica Nacional, Maestro López y Cruz Roja Argentina, 5016 Córdoba, Argentina; Departamento de Físico-Química, Facultad de Ciencias Químicas, Universidad de Concepción, Casilla 3-C, ...

5 MS 147-06 Ntoifar

African Journals Online (AJOL)

Damary

2006-10-01

Oct 1, 2006 ... used plant-based methods while 31 (35.2%) used various petroleum oils. The rest of the ... Conclusion: Most plants used against mosquitoes in the area are known potent mosquito repellents but others identified in the study ..... Rojas E. and Scorza J.V. The use of lemon essential oil as a sandfly repellent.

Coprophilous Streptomycetes and Fungi - Food Sources for Enchytraeid Worms (Enchytraeidae)

Czech Academy of Sciences Publication Activity Database

Krištůfek, Václav; Nováková, Alena; Pižl, Václav

2001-01-01

Roč. 46, č. 6 (2001), s. 555-558 ISSN 0015-5632 R&D Projects: GA AV ČR IAA6066001 Institutional research plan: CEZ:AV0Z6066911 Keywords : food selection * vermicomposting * Eisenia and rei Subject RIV: EH - Ecology, Behaviour Impact factor: 0.776, year: 2001

1608-IJBCS-Article-André T Kabré

African Journals Online (AJOL)

hp

Eisenia fetida (Oligochaeta) and Oreo- chromis mossambicus (Cichlidae) in. South Africa. Acta Tropica, 63: 1-14. Chiayvareejja S, Rittibhonbhum N, Hong- promyart M,Wiriyachitra P. 1997. Toxic- ity of the Thai piscicidal plant, Maesa ramentacea, to freshwater fishes in ponds. Aquaculture, 158: 229-234. Davies W. 1983.

EGRESADOS DEL REAL COLEGIO DE MINERÍA EN LOS INICIOS DE LA REVOLUCIÓN DE INDEPENDENCIA DE MÉXICO. 1810. EGRESADOS DEL REAL COLEGIO DE MINERÍA EN LOS INICIOS DE LA REVOLUCIÓN DE INDEPENDENCIA DE MÉXICO. 1810.

Directory of Open Access Journals (Sweden)

Isauro Rionda Arreguín

2012-02-01

Full Text Available The Real Colegio de Mineria or Real Seminario de Mineria or Colegio Metalico was founded in 1792 and in january the 1st of that year admitted the first students. Several of their graduates during the firs decade of the XIX century worked in the mining industry in Guanajuato and were involved in the insurrection movement, among them were: José Antonio Rojas, Casimiro Chowell, Rafael Dávalos, y Ramón Fabié y Mariano Jiménez.Se funda en 1792 el Real Colegio de Minería o Real Seminario de Minería, o Colegio Metálico; institución que abre sus puertas a los primeros alumnos, el día primero de enero de ese año. Esos egresados del Real Colegio de Minería que en la primera década del siglo XIX trabajaron en la minería guanajuatense fueron: José Antonio Rojas, Casimiro Chowell, Rafael Dávalos, y Ramón Fabié y Mariano Jiménez.

Vejamen de Alfonso de Batres para la Academia de 1638 (Manuscrito inédito. Estudio y edición crítica

Directory of Open Access Journals (Sweden)

Julio, Teresa

2013-06-01

Full Text Available During February 1638 the Fiestas de Carnestolendas were celebrated at the Buen Retiro Palace, Madrid. Among the programmed activities, a literary gathering was held, open to numerous poets and courtiers. From this gathering three vejamenes have been preserved: those of Alfonso de Batres, Antonio Coello and Francisco de Rojas Zorrilla. In this paper we analyze and annotate the vejamen that Batres read before His Majesties in the Royal Salon of the Retiro Palace on February 11, 1638.Durante el mes de febrero de 1638 se celebraron en el palacio del Buen Retiro las fiestas de Carnestolendas. Entre las actividades programadas, se realizó una academia literaria en la que participaron poetas y cortesanos. De ella se conservan tres vejámenes: el de Alfonso de Batres, el de Antonio Coello y el de Francisco de Rojas Zorrilla. En el presente artículo se analiza y edita críticamente el vejamen que Batres defendió ante sus majestades en el Salón Real del Retiro el 11 de febrero de 1638.

Riboflavin content in autofluorescent earthworm coelomocytes is species-specific.

Directory of Open Access Journals (Sweden)

Joanna Homa

2007-01-01

Full Text Available We have recently shown that a large proproportion of earthworm coelomocytes exhibit strong autofluorescence in some species (Dendrobaena veneta, Allolobophora chlorotica, Dendrodrilus rubidus, Eisenia fetida, and Octolasion spp., while autofluorescent coelomocytes are very scarce in representatives of Lumbricus spp. and Aporrectodea spp. Riboflavin (vitamin B2 was identified as a major fluorophore in Eisenia jetida coelomocytes. The main aim of the present experiments was to quantify riboflavin content in autofluorescent coelomocytes (eleocytes from several earthworm species through a combination of flow cytometric and spectrofluorometric measurements. Spectrofluorometry of coelomocyte lysates showed that riboflavin was non-detectable in the coelomocytes of Aporrectodea spp. and Lumbricus spp., but was a prominent constituent of lysates from species with autofluorescent eleocytes. In the latter case, riboflavin content was the highest in E. fetida, followed by Octolasion spp. > A. chlorotica > D. rubidus. The riboflavin content of coelomocytes correlates positively with eleocyte autofluorescence intensity measured by flow cytometry and visible with fluorescence microscopy.

Comparative toxicity of chemicals to earthworms

Energy Technology Data Exchange (ETDEWEB)

Callahan, C.A.; Shirazi, M.A. (Environmental Protection Agency, Corvallis, OR (United States)); Neuhauser, E.F. (Niagara Mohawk Power Corp., Syracuse, NY (United States))

1994-02-01

The concentration-response (mortality) relationships of four species of earthworms, Eisenia fetida (Savigny), Allolobophora tuberculata (Eisen), Eudrilus eugeniae (Kinberg), and Perionyx excavatus (Perrier) are summarized for 62 chemicals and two test protocols. A Weibull function is used to summarize these data for each chemical in terms of sensitivity and toxicity, in addition to the LC50. The estimation of the Weibull parameters a and k summarize the entire concentration-response relationship. This technique should be applicable to a variety of testing protocols with different species whenever the goal is summarizing the shape of the concentration-response curves to fully evaluate chemical impact on organisms. In some cases for these data four orders of magnitude separate LC50s of the soil test and the contact test for the same chemical and species. All four species appear to be similar in range of toxicity and tolerance to these chemicals, suggesting that Eisenia fetida and may be representative of these four species and these chemicals.

Microbial Environment Affects Innate Immunity in Two Closely Related Earthworm Species Eisenia andrei and Eisenia fetida

Czech Academy of Sciences Publication Activity Database

Dvořák, Jiří; Mančíková, Veronika; Pižl, Václav; Elhottová, Dana; Šilerová, Marcela; Roubalová, Radka; Škanta, František; Procházková, Petra; Bilej, Martin

2013-01-01

Roč. 8, č. 11 (2013) E-ISSN 1932-6203 R&D Projects: GA MŠk(CZ) EE2.3.20.0055; GA MŠk(CZ) EE2.3.30.0003 Institutional support: RVO:61388971 ; RVO:60077344 Keywords : POLYMERASE-CHAIN-REACTION * CELOMIC FLUID * TRANSPLANTATION IMMUNITY Subject RIV: EE - Microbiology, Virology; EC - Immunology (BC-A) Impact factor: 3.534, year: 2013

Modelling global change impacts on soil carbon contents of agro-silvo-pastoral Mediterranean systems

Science.gov (United States)

Lozano-García, Beatriz; Muñoz-Rojas, Miriam; Parras-Alcántara, Luis

2016-04-01

To assess the impact of climate change on soil organic C (SOC) stocks in agro-silvo-pastoral environments, different models have been applied worldwide at local or regional scales, such as as RothC (Francaviglia et al., 2012) or CENTURY (Alvaro-Fuentes et al., 2012). However, some of these models may require a high number of input parameters or can underestimate the effect of soil depth. CarboSOIL (Muñoz-Rojas et al., 2013) is an empirical model based on regression techniques and developed to predict SOC contents at standard soil depths (0-25, 25-50 and 50-75 cm) under a range of climate and/or land use change scenarios. CarboSOIL has been successfully applied in different Mediterranean areas ,e.g. Southern Spain (Muñoz-Rojas et al., 2013; Abd-Elmabod et al., 2014), Northern Egypt (Muñoz-Rojas et al., 2014) and Italy (Muñoz-Rojas et al., 2015). In this study, CarboSOIL was applied in the Cardeña and Montoro mountain range Natural Park. This area covers 385 km2 and is located within Sierra Morena (Córdoba, South Spain) and has a semiarid Mediterranean climate. It is characterized by agro-silvo-pastoral systems. The Mediterranean evergreen oak woodland (MEOW-dehesa) is savanna-like open woodland ecosystem characterized by silvopastoral uses, being an ancient human modified Mediterranean landscape (Corral-Fernández et al., 2013; Lozano-García and Parras-Alcántara 2013). The most representative soils in the Cardeña and Montoro mountain range Natural Park are Cambisols, Regosols, Leptosols and Fluvisols. These soils are characterized by low fertility, poor physical conditions and marginal capacity for agricultural use, together with low organic matter (OM) content due to climate conditions (semiarid Mediterranean climate) and soil texture (sandy). The model was applied at different soil depths: 0-25, 25-50 and 50-75 cm (Parras-Alcántara et al., 2015) considering land use and climate changes scenarios based on available global climate models (IPPC, 2007). A

Bulletin of Materials Science | Indian Academy of Sciences

Indian Academy of Sciences (India)

V Renteria. Articles written in Bulletin of Materials Science. Volume 38 Issue 1 February 2015 pp 29-40. Yttrium deposition on mesoporous TiO2: textural design and UV decolourization of organic dyes · M L Ojeda C Velasquez V Renteria A Campero M A García-Sánchez F Rojas · More Details Abstract Fulltext PDF.

Translations on Narcotics and Dangerous Drugs No. 305

Science.gov (United States)

1977-06-17

prosecutor; Augusto Caro Rojas, court reporter; Rodolfo Carvajal Florez, municipal employee; Orlando Carrascal Claro, inspector; Jesus Antonio Carrillo...Arturo Melo Diaz, judge; Rafael Mendez Buendia, judge; Tirso Mendez Herrera, professor; Rubiela Maria Mendez Mendez , judge; Luis Orlando Mendez ...Misael Roa Fonseca, jail guard; Jose Ignacio Roa Mendez , policeman; Jose Anibal Robles Alvarado, judge; Jorge Rocha Mahecha, govern- ment inspector

Chemical profiling of oil and PAH polluted soil and drainage water

DEFF Research Database (Denmark)

Boll, Esther Sørensen

of a polar group. I have worked with two test organisms that utilize this degradation pathway, the earthworm Eisenia Fetida and the terrestrial fungus Cunninghamella elegans. E. fetida was exposed to the two PAHs, phenanthrene and pyrene, in a hydroponic culture. E. fetida was extensively able to transform...

Effet de la densité de charge et de différents types de substrat sur la ...

African Journals Online (AJOL)

performances de production du ver du fumier Eisenia foetida durant 8 semaines. Les fumiers ... Leur importance dans la gestion de la fertilité des terres ... étude a été initiée pour (1) mesurer la vitesse .... Contrôle de croissance numérique et.

Vermiremediation of soils contaminated with mixture of petroleum ...

African Journals Online (AJOL)

In this paper, vermiremediation, a biological technique was utilized in order to clean-up soil contaminated with gasoline, diesel and spent engine oil using an earthworm - Eisenia fetida. The contaminated soils were analyzed for the total petroleum hydrocarbon (TPH) level every 24 hours over a period of 120 hours using ...

The Soil Program of the Restoration Seedbank Initiative: addressing knowledge gaps in degraded soils for use in dryland restoration

Science.gov (United States)

Muñoz-Rojas, Miriam; Bateman, Amber; Erickson, Todd E.; Turner, Shane; Merritt, David J.

2017-04-01

(i) the determination of ecophysiological indicators influencing drought responses of arid native plants in reconstructed soils (Bateman et al, 2016), ii) the analysis of the influence of climate and edaphic factors in the recruitment of arid zone seedlings (Muñoz-Rojas et al., 2016a) and (ii) the evaluation of soil physicochemical and microbiological indicators to assess functionality of restored soils in degraded semiarid ecosystems (Muñoz-Rojas et al., 2016b). Here, we summarize our latest results in the soil program of the RSB, and propose recommendations for integrating soil science in cost-effective landscape-scale restoration practices in ecosystems worldwide. References Bateman A, Lewandrowski W, Stevens J, Muñoz-Rojas M (2016b) Ecophysiological indicators to assess drought responses of arid zone native seedlings in reconstructed soils. Land Degradation & Development, in press, DOI: 10.1002/ldr.2660. Kildisheva OA, Erickson TE, Merritt DJ, Dixon KW (2016), Setting the scene for dryland recovery: an overview and key findings from a workshop targeting seed-based restoration. Restoration Ecology 24, S36-S42. Muñoz-Rojas M, Erickson TE, Dixon KW, Merritt DJ (2016) Soil quality indicators to assess functionality of restored soils in degraded semiarid ecosystems. Restoration Ecology 24, S43-S52. DOI: 10.1111/rec.12368 Muñoz-Rojas M, Erickson TE, Martini DC, Dixon KW, Merritt DJ (2016a) Climate and soil factors influencing seedling recruitment of plant species used for dryland restoration. SOIL 2, 287-298. DOI: 10.5194/soil-2016-25

Bulletin of Materials Science | Indian Academy of Sciences

Indian Academy of Sciences (India)

M L Ojeda1 C Velasquez1 V Renteria1 A Campero2 M A García-Sánchez2 F Rojas2. Centro de Investigación en Nanociencias y Nanotecnología, Universidad de Guadalajara, Universitario de los Valles, CP 46600 Ameca, Jalisco, México; Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, ...

Proper Motion Of Teh Magellanic Clouds Using SPM

Science.gov (United States)

2013-01-01

1.05,−1.03) ± (0.30, 0.29) masa año−1, basado en dos muestras de 3800 y 769 es- trellas gigantes rojas de la LMC y SMC respectivamente. Una porción...justment also helps to correct residual distortions, as they are statistically smoothed out in the average frame. To avoid frames drifting away from the

Eventos de Mayo (May Events).

Science.gov (United States)

Toro, Leonor; Pla, Myrna

Designed as a resource for teachers, this booklet, written in Spanish, contains brief information on seven May events: La Semana de la Educacion (first Friday in May), Harry S. Truman (May 8), Dia de las Madres (second Sunday in May), Luis Llorens Torres (May 14), La Cruz Roja (May 21), John F. Kennedy (May 29), and El Dia De Conmemoracion (May…

Inadequate taxonomy and highly divergent COI haplotypes in ...

African Journals Online (AJOL)

These focal groups, including two laboratory cultures and one field population, have been referred to as Eisenia fetida in the literature. A previous molecular study had already helped to establish that one of the two laboratory groups was a population of E. fetida's sister species E. andrei. In the present contribution, analyses ...

Organic amendments as restoration techniques in degraded arid and semiarid systems: A review

Science.gov (United States)

Hueso-González, Paloma; Muñoz-Rojas, Miriam

2017-04-01

There is an increasing concern at the global scale about interrelated environmental problems such as soil degradation, desertification, erosion, and climate change impacts (Hueso-Gonzalez et al., 2014). Indiscriminate use of agro-chemicals, excessive and deep tillage, excessive irrigation, among many others factors, have largely contributed to soil degradation, particularly in arid and semi-arid areas (Lal, 2008). Soil is an essential non-renewable resource with extremely slow formation and regeneration potential (Muñoz-Rojas et al., 2016a and c, Martínez-Murillo et al., 2016). The decline in organic matter content of many soils is becoming a major cause of soil degradation, particularly in dryland regions (Muñoz-Rojas et al., 2016b) where low soil fertility cannot maintain sustainable production in many cases (Hueso-González et al., 2015). The use of soil organic amendments is a common practice in agricultural management and land restoration that can help to improve physical and chemical soil properties, soil structure, temperature and humidity conditions, as well as nutrient contents which are essential for plant growth (Guerrero et al., 2001). Under degraded conditions, several studies have shown their benefits for improving soil physical, chemical and biological properties (Jordan et al., 2010 and 2011). However, there are many research gaps in the knowledge of the effects of climatic conditions on their application, as well as the adequate types of amendment and doses and decomposition rates, (Hueso-Gonzalez,2016). All these factors are crucial for the success in their application. Here, we review long-term experiments worldwide studying the benefits associated with the application of organic materials, particularly, in restoration of arid and semiarid ecosystems together with the possible threats and risks that can result from their use. We will specifically adress: (1) type of amended and benefits arising from their use, (2) application methods and more

CARACTERIZACIÓN DE ARCILLAS Y PREPARACIÓN DE PASTAS CERÁMICAS PARA LA FABRICACIÓN DE TEJAS Y LADRILLOS EN LA REGIÓN DE BARICHARA, SANTANDER

Directory of Open Access Journals (Sweden)

JOSÉ DARÍO SANTOS AMADO

2011-01-01

Full Text Available El objetivo de este trabajo fue caracterizar química, mineralógica, granulométrica, plástica y térmicamente tres arcillas, denominadas "Cascajo" (C, "Roja" (R y "Amarilla" (A, procedentes de Barichara, Santander. Así como obtener pastas cerámicas aptas para la fabricación de ladrillos y tejas. Para lo cual se fabricaron probetas cerámicas por prensado, las cuales se sinterizaron a 1050ºC y posteriormente se caracterizaron física y mecánicamente. Los resultados demostraron que la pasta de arcilla "Roja", con contenido de agua entre 24 y 38%, es la más adecuada para la fabricación de tejas; ya que los cerámicos presentan baja absorción de agua (8.2% y alto módulo de ruptura (9.3 MPa. Para la obtención de ladrillos se recomienda la pasta con mezcla de arcillas de 55% "Cascajo" y 45% "Amarilla",con contenido de agua entre 23 y 32%, obteniéndose cerámicos con absorción de agua menor al 15% y módulo de ruptura mayor a 5 MPa.

Entre Venus y Marte: el viaje del héroe en Desertor (Between Venus and Mars: The Journey of the Hero in Desertor

Directory of Open Access Journals (Sweden)

Grethel Ramírez Villalobos

2015-11-01

Full Text Available En este artículo se estudia la figura del héroe en la novela Desertor, del costarricense Juan Ramón Rojas. Este relato tiene su ambiente en El Salvador y analiza algunos de los principales hechos históricos de la década de 1980. El protagonista del relato se ve impulsado a revisar sus propias convicciones políticas y emprende un viaje, que resulta análogo a los periplos de los héroes mitológicos. A partir del examen de la conformación del héroe, se discuten los simbolismos literarios y los principales asuntos tratados en la novela. This article addresses the figure of the hero in the novel Desertor, by the Costa Rican Juan Ramón Rojas. It takes place in El Salvador and analyzes some of the main historical events of the 1980s. The protagonist is forced to review his own political convictions and sets out on a journey analogous to those of mythological heroes. Considering the analysis of the conformation of the hero, literary symbolism and the main topics of the novel are examined.

Distribution of sexually transmitted diseases and risk factors by work locations among female sex workers in Tijuana, Mexico.

Science.gov (United States)

Rusch, Melanie L A; Brouwer, Kimberly C; Lozada, Remedios; Strathdee, Steffanie A; Magis-Rodríguez, Carlos; Patterson, Thomas L

2010-10-01

Sex work is regulated in the Zona Roja (red light district) in Tijuana, Mexico, where HIV and sexually transmitted disease (STD) prevalence is high among female sex workers (FSWs). We examined the spatial distribution of STDs by work venue among FSWs in Tijuana. FSWs aged 18 years and older who reported unprotected sex with ≥ 1 client in the past 2 months underwent testing for HIV, syphilis, gonorrhea, and Chlamydia. HIV/STDs were mapped by venue (i.e., bar, hotel) and Getis-Ord Gi statistics were used to identify geographic hotspots. High-risk venues were then identified using a standardized STD ratio (high risk defined as a ratio ≥ 1.25). Logistic regression was used to assess correlates of working at a high risk venue. Of 474 FSWs, 176 (36.4%) had at least 1 bacterial sexually transmitted infection (STI); 36 (7.6%) were HIV-positive. Within the Zona Roja, 1 venue was identified as a geographic "hotspot," with a higher than expected number of HIV/STD-positive FSW (P Tijuana. Structural interventions that focus on sex work venues could help increase STI diagnosis, prevention, and treatment among FSWs in Tijuana.

Excellent N-fixing and P-solubilizing traits in earthworm gut-isolated bacteria: A vermicompost based assessment with vegetable market waste and rice straw feed mixtures.

Science.gov (United States)

Hussain, Nazneen; Singh, Archana; Saha, Sougata; Venkata Satish Kumar, Mattaparthi; Bhattacharyya, Pradip; Bhattacharya, Satya Sundar

2016-12-01

Vermicomposting is a dependable waste recycling technology which greatly augments N and P levels mainly through microbial action. This paper aims to identify efficient N-fixing (NFB) and P-solubilizing (PSB) bacteria from earthworm intestines. Various combinations of vegetable market waste, rice straw, and cowdung were fed to two earthworm species (Eisenia fetida and Perionyx excavatus). Total organic C decreased, pH shifted towards neutrality, and NPK availability, and microbial (NFB, PSB, and total bacteria) population increased remarkably during vermicomposting with E. fetida. Therefore, 45 NFB and 34 PSB strains isolated from Eisenia gut were initially screened, their inter-dominance assessed, and 8 prolific strains were identified through 16SrRNA sequencing. Interestingly, two novel N-fixing strains of Kluyvera ascorbata emerged as an efficient biofertilizer candidate. Moreover, both N-fixing and P-solubilizing strains of Serratia and Bacillus were isolated from earthworm gut. All the isolated strains significantly improved soil health and facilitated crop growth as compared to commercial biofertilizers. Copyright © 2016 Elsevier Ltd. All rights reserved.

Investigation of Vermiwash Obtained from California Red Worm Which Fed Cow Dung in terms of Trace and Nutrient Elements

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Turan Yüksek

2017-08-01

Full Text Available One of the most important problems facing sustainable agriculture is the supply of quality fertilizers and the problems encountered in the application of fertilizers. In the last years, while trying to increase the amount of fertilizers obtained by natural methods, On the other hand it has been observed that studies for obtaining different types (solid natural, liquid natural, bio-natural etc. of fertilizer have continued increasing. The aim of this study is to determine time depend (30 days, 31-60 days changes of some nutrients and metals in vermiwash obtained from Eisenia fetida fed with fermented natural cow dung. For this purpose, Eisenia fetida were fed with fermented cow dung which obtained from cows grazing in natural habitat and obtained vermiwash were analyzed to determined some macro and micronutrient elements. As a result of the study, while the values of N, K, Ca, Mg, Na, Fe, Ni, Cu, Zn, Hg, Sb in vermiwash decreased at a statistically significant level depending on the time; Co, As, Ag, Cd, Pb, Al, Se, Mo, Li and Be were increased at statistically significant levels.

Producción de plantas de camu camu con diferentes sustratos orgánicos en camas de vivero convencional

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Carlos Abanto Rodriguez

2013-01-01

Full Text Available El objetivo fue verificar el desarrollo inicial de plantones de camu camu con diferentes sustratos orgánicos en camas de vivero convencional con riego por microaspersión y manejo de sombra con malla Chromatine® color rojo con 50% de luz. Se utilizó material genético proveniente de plantas madre del Banco de Germoplasma de camu camu del INIA-Iquitos, las semillas fueron germinadas en aserrín descompuesto y mantenidas por un periodo de 40 días, luego se realizó el repicado con 10 cm de altura en los diferentes sustratos según los tratamientos en camas de vivero con dimensiones de 1,20 m de ancho x 10 m de largo y con una profundidad de 30 cm. Después de realizar las evaluaciones de altura (cm y diámetro basal (mm durante un periodo de 120 días, se encontró que el sustrato gallinaza se consolida como el sustrato de mayor eficiencia en el desarrollo de plantas de camu camu seguido por el sustrato humus de lombriz.

| CTIO

Science.gov (United States)

de formación de estrellas, elipses inmóviles compuestas principalmente de antiguas estrellas rojas en estas agrupaciones densas son comunes, lo que lleva a la rápida formación de estrellas, pero Estrellas. (Crédito de la imagen: Investigación de la Energía Oscura) Ver el video de Fermilab SNe

NRL Fact Book

Science.gov (United States)

1988-05-01

interests are centered on signal processing and the physics of underwater acoustic propagation, ambient noise, and reverberation. Mr. Rojas is a member...Airborne underwater acoustics Bottom-limited acoustics Arctic underwater acoustics Propagation Noise Ambient noise measurements and modeling Spectral...Multispectral image correlation Space sensor and mission analysis CROWN" STALK Time, ms (100 ms after impactO Time-history of the . radar backscatter from a

Cultivo en mesocosmos: un modelo de acuicultura marina para ser aplicado en Colombia

OpenAIRE

Casas R., José Joaquín

2001-01-01

Durante las últimas dos décadas, la acuicultura en Colombia ha crecido esencialmente en el campo de la acuicultura de aguas dulces. La introducción de especies como la tilapia roja, la trucha arco iris, el salmón coho y cultivos con algunas especies nativas como la cachama, yamú y bocachico, han estimulado el desarrollo de este sector económico

Arte rupestre paleolítico en la Cueva de Cordoveganes I (Puertas de Vidiago, concejo de Llanes, Asturias. Hallazgo y primera documentación de un nuevo conjunto parietal en la región cantábrica

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Silvia SANTAMARÍA SANTAMARÍA

2011-04-01

Full Text Available En el presente artículo se presenta un nuevo conjunto rupestre paleolítico recientemente localizado en la Cueva de Cordoveganes I. El mismo consta de diversas pinturas rojas, entre las que destaca una cierva realizada con trazo tamponado y diversas pinturas negras, realizadas con carbón vegetal, con un notable panel compuesto de grandes signos formados por trazos lineales.

USMILGP Colombia: Transforming Security Cooperation in the Global War on Terrorism

Science.gov (United States)

2006-12-01

lives over the next 18 years in what became known as La Violencia .2 Aside from the catastrophic number of deaths, La Violencia was also responsible...dictator Gustavo Rojas Pinilla charged electoral fraud in the presidential election of April 19, 1970. In 1997, various illegitimate paramilitary...conflicts between Liberal and Conservative militias and assorted bandit gangs that had characterized La Violencia (1948-1958) had transmogrified in the

Comparative study of the CCF-like pattern recognition protein in different Lumbricid species

Czech Academy of Sciences Publication Activity Database

Šilerová, Marcela; Kohlerová, Petra; Josková, Radka; Josens, G.; Beschin, A.; De Baetselier, P.; Bilej, Martin

2006-01-01

Roč. 30, - (2006), s. 765-771 ISSN 0145-305X R&D Projects: GA ČR GA310/04/0806; GA ČR GD310/03/H147; GA AV ČR IAA5020208 Institutional research plan: CEZ:AV0Z50200510 Keywords : lubricid * eisenia fetida * ccf Subject RIV: EE - Microbiology, Virology Impact factor: 3.399, year: 2006

Características fisicoquímicas y composición en ácidos grasos del aceite extraído de semillas de tomate de árbol (Cyphomandra betacea Sendt variedades roja y amarilla

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Linares, Oscar

2004-12-01

Full Text Available Tree tomato ( Cyphomandra betacea Sendt is a Solanaceae originating from Peru . In Venezuela , it is cultivated in the Andean region and Aragua state. The objective of this research was to determine some physicochemical characteristics of oil extracted from tree tomato seeds of red (R and yellow (Y varieties. The parameters evaluated were: free oleic acidity (%, R= 1.1; Y= 1.1, saponification number (mg KOH/g, R= 195.3; Y= 196.2, iodine index (cg I2/g, R= 143.3; Y= 142.0, peroxide value (meq O2/kg, R= 1.5; Y= 1.4, unsaponifiable matter (g/kg, R= 19.6; Y= 20.3, refraction index at 40 ºC (R= 1.4720; Y= 1.4710, relative density at 20 ºC (R= 0.9236; Y= 0.9240. The fatty acid composition showed (%: palmitic acid (R= 7.7; Y= 7.4, stearic acid (R= 3.5; Y= 3.1, oleic acid (R= 16.3; Y= 17.7, linoleic acid (R= 69.0; Y= 69.3, and linolenic acid (R= 3.5; Y= 2.5. Tree tomato oil is recommended as a possible nutraceutic agent and salad-oil.El tomate de árbol (Cyphomandra betacea Sendt pertenece a la familia Solanaceae, originaria de Perú. En Venezuela, se cultiva en la región andina y en el estado Aragua. El objetivo de esta investigación fue determinar algunas características físico-químicas del aceite extraído de las semillas de este fruto de las variedades roja (R y amarilla (A, que permitan definir su utilidad como posible materia prima oleaginosa. Los parámetros evaluados fueron: acidez libre oleica (% (R: 1,1 y A: 1,1; índice de saponificación (mg KOH/g (R: 195,3 y A: 196,2; índice de yodo (cg I2/g (R: 143,3 y A: 142,0; índice de peróxidos (meq O2/kg (R: 1,5 y A: 1,4; materia insaponificable (g/kg (R: 19,6 y A: 20,3; índice de refracción a 40 ºC (R: 1,4720 y A: 1,4710 y densidad relativa a 20 ºC (R: 0,9236 y A: 0,9240. La composición de ácidos grasos determinada fue: palmítico (R: 7,7 y A: 7,4; esteárico (R: 3,5 y A: 3,1; oleico (R: 16,3 y A: 17,7; linoleico (R: 69,0 y A: 69,3 y linolénico (R: 3,5 y A: 2,5. La presencia de �

On ‘war task’ and ‘peace work’. The Dutch East Indies Red Cross between the colonial wars and the Second World War

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Martínez-Antonio, Francisco Javier

2014-06-01

Full Text Available From the end of the 19th to the beginning of the 20th century, Morocco’s modernizing projects included plans for signing up to the Geneva Convention and creating a local Red Cross/Crescent society. These plans initially stemmed from the convergence of Moroccan administrative/military reforms and Spanish “regenerationist” interventions. They ran parallel to developments in leading Islamic countries such as Egypt, Persia and the Ottoman Empire though they would have to wait till the country’s independence from Franco-Spanish domination in 1956 to become a reality. Beyond their lack of actual results, those early initiatives would serve as legal ground for Morocco’s “humanitarian sovereignty”, tacitly confirmed by the provisions of the Algeciras Act of 1906. In the following two decades, the resilience of this sovereignty would reveal itself in the sustained competition between the Spanish and the French Red Cross for “humanitarian hegemony” in the country, and also in the repeated and nearly successful demands to establish a local Red Cross/Crescent that were made to the International Committee of the Red Cross by the insurgent leader Abdelkrim during the so-called Rif War.Hacia finales del siglo XIX y comienzos del XX, los proyectos de modernización de Marruecos incluyeron planes de adhesión a la Convención de Ginebra y de creación de una sociedad local de la Cruz/Media Luna Roja. Estos planes surgieron de la convergencia entre las reformas administrativas/militares marroquíes y las intervenciones “regeneracionistas” españolas. Transcurrieron en paralelo con los desarrollos en países islámicos líderes como Egipto, Persia y el Imperio Otomano, aunque habrían de esperar para hacerse realidad hasta la independencia del país de la dominación franco-española en 1956. Más allá de la falta de resultados concretos, estas tempranas iniciativas servirían como marco legal a la “soberanía humanitaria” de Marruecos

Polymorphism of the prolactin gene (PRL) and its relationship with ...

African Journals Online (AJOL)

enoh

2012-04-10

Apr 10, 2012 ... cattle. Edy Alfonso1, Reyna Rojas1, José G. Herrera1, María E. Ortega1, Clemente Lemus2*, César. Cortez1, Jaime Ruiz3, René Pinto4 and Heriberto Gómez4 ..... Ciencias Médicas y Nutrición Salvador Zubirán. México. Rev. Invest. Clín. 57(3): ISSN 0034-8376. Miller SA, Dykes DD, Poletsky HF (1988).

REDESCUBRIMIENTO DE MYRRHINIUM ATROPURPUREUM VAR. OCTANDRUM (MYRTACEAE: MYRTINAE EN COLOMBIA

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PARRA-O. CARLOS

2003-12-01

Full Text Available Se documenta el redescubrimiento de Myrrhinium atropurpureum var. octandrum(Myrteae DC., Myrtaceae, taxón prácticamente desconocido en Colombia. Se presentala descripción del taxón, así como notas sobre su distribución en Colombia, elhábitat en que se desarrolla y su posible estatus dentro de las categorías de la ListaRoja de la UICN.

Activity of earthworm in Latosol under simulated acid rain stress

Science.gov (United States)

Jia-En Zhang; Jiayu Yu; Ying Ouyang

2015-01-01

Acid rain is still an issue of environmental concerns. This study investigated the impacts of simulated acid rain (SAR) upon earthworm activity from the Latosol (acidic red soil). Laboratory experiment was performed by leaching the soil columns grown with earthworms (Eisenia fetida) at the SAR pH levels ranged from 2.0 to 6.5 over a 34-day period....

Remedial Investigation of Contaminant Mobility at Naval Weapons Station, Concord, California.

Science.gov (United States)

1986-01-01

Houghton. 1983. Heavy Metal Uptake by Agronomic Crops Grown on Contaminated Soils and Cyperus esculentus Grown on Oxidized and Reduced Contaminated Soils...Respiratory Metabolism and Haemoglobin Concentration in an Earthworm Eisenia foetida (SAVIGNY 1826) Under Exposure of Lead Intoxication . Acta...Comparison of the Heavy-Metal Uptake of Cyperus esculentus and of Agronomic Plants Grown on Contaminated Sediments. Miscellaneous Paper D-83-1. US

Variación de diferentes parámetros de humificación durante dos tipos de compostaje con residuos lignocelulósicos de rosas

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Diana María Farías Camero

2010-11-01

Full Text Available Se llevaron a cabo dos procesos de compostaje a partir de residuos lignocelulósicos de rosas durante 165 días aproximadamente. En uno de los procesos la descomposición del material fue realizada sólo por microorganismos (compostaje directo y en el otro fuera de los microrganismos se adicionaron lombrices de tierra Eisenia foetida (compostaje indirecto.

Contributions to Earhworm (Clitellata; Annelida Fauna of Turkish Thrace

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İbrahim Mete Mısırlıoğlu

2017-06-01

Full Text Available In this study, earthworm specimens gathered from 2 different localities which are located in Kırklareli-Babaeski were identified. At the end of the study, 3 species belonging to 2 genus were found: Aporrectodea rosea (Savigny, 1826, Aporrectodea trapezoides (Dugès, 1828, Eisenia fetida (Savigny, 1826. It is the first record of Aporrectodea rosea in Turkish Thrace.

OBTENCIÓN DE UN ALIMENTO EXTRUIDO PARA TILAPIA ROJA (Oreochromis spp UTILIZANDO ENSILAJE BIOLÓGICO DE PESCADO A OBTENÇÃO DE UMA RAÇÃO EXTRUSADA PARA A TILÁPIA VERMELHA (Oreochromis spp UTILIZANDO SILAGEM DE PESCADO BIOLÓGICA OBTAINING AN EXTRUDED NOURISHMENT FOR RED TILAPIA (Oreochromis ssp USING FISH BIOLOGICAL SILAGE

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JAIDER OSWALDO PANTOJA

2011-12-01

Full Text Available En la represa 'La Salvajina', municipio de Suárez (Cauca, la transformación de Tilapia roja (Oreochromis spp deja como subproductos mayoritarios cabezas, huesos, piel, escamas y vísceras, los cuales a través del proceso de ensilaje pueden brindar una alternativa proteica en la formulación de alimentos para peces. La presente investigación obtuvo pelets extruidos para tilapia roja en fase de engorde, sustituyendo parcialmente harina de pescado por ensilaje biológico. Las variables de respuesta del pelet (propiedades físicas, permitieron a través de un diseño factorial 23, evaluar los parámetros del proceso de extrusión (temperatura °C, velocidad de tornillo rpm y humedad %. Los resultados indicaron significancia por parte de factores e interacciones, siendo la humedad el factor de mayor incidencia. El punto medio (PM del diseño, formulado con 15% de ensilaje y procesado con niveles medios (125°C, 170 rpm, 28%, presentó un porcentaje superior al 90% de flotabilidad, además de resultados aceptables en el índice de expansión, densidad específica, índice de absorción de agua, estabilidad en agua y durabilidad; logrando condiciones favorables para la obtención de un alimento con propiedades físicas similares a las de un alimento comercial.Na barragem, 'A Salvajina', no municipio de Suárez (Cauca, a transformação da Tilápia Vermelha (Oreochromis spp, deixa como principais produtos: cabeças, ossos, pele, escamas e as vísceras, que através do processo de ensilagem podem constituir uma alternativa de proteína na formulação de rações para peixes. Esta pesquisa obteve aglomerados extrusados para tilápia vermelha na engorda, substituindo parcialmente a farinha de peixe por silagem biológica. As variáveis de resposta de pelet (propriedades físicas, permitiram através de um planejamento fatorial 23, avaliar os parâmetros de processo de extrusão (temperatura °C, velocidade do parafuso rpm e umidade %. Os resultados

Areúsa en La Celestina: De la Comedia a la Tragicomedia

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Morros Mestres, Bienvenido

2010-06-01

Full Text Available Rojas seems to Areúsa to attribute two different papers in the work. In the Comedy he presents it as the friend of a military man with the one that lives together in a regimen of concubinage allowed by the law and that was the most similar thing to the marriage. In the Tragicomedy, on the other hand, he turns her into a clandestine prostitute who takes part of the corruption of the justice to make and to undo to her whim. In five interpolated cars he ends up by granting her a function very similar to the one that had had the deceased Celestina.

Rojas parece atribuir a Areúsa dos papeles distintos en la obra. En la Comedia la presenta como la amiga de un militar con el que cohabita en un régimen de amancebamiento permitido por la ley y que era lo más parecido al matrimonio. En la Tragicomedia, en cambio, la convierte en una prostituta clandestina que participa de la corrupción de la justicia para hacer y deshacer a su antojo. En los cinco autos interpolados le acaba otorgando una función muy similar a la que había tenido la fallecida Celestina.

Historical aspects of Mexican psychiatry.

Science.gov (United States)

Bayardo, Sergio Javier Villaseñor

2016-04-01

Mexican psychiatry initiated since pre-Hispanic times. Historically, treatments were a mixture of magic, science and religion. Ancient Nahuas had their own medical concepts with a holistic view of medicine, considering men and cosmos as a whole. The first psychiatric hospital appeared in 1566 and a more modern psychiatric asylum emerged until 1910. International exchanges of theoretical approaches started in the National University with the visit of Pierre Janet. There were other important figures that influenced Mexican psychiatry, such as Erich Fromm, Henri Ey, Jean Garrabé and Yves Thoret. Regarding Mexican psychiatrists, some of the most important contributors to Mexican psychiatry were José Luis Patiño Rojas, Manuel Guevara Oropeza and Ramón de la Fuente Muñiz. This article includes excerpts from "Clinical Psychiatry", a book by Patiño Rojas where he tries to understand and describe the inner world experienced by patients with schizophrenia; also, the thesis conducted by Guevara Oropeza ("Psychoanalisis"), which is a critical comparison between the theories of Janet and Freud. Finally, we include "The study of consciousness: current status" by Ramón de la Fuente, which leads us through the initial investigations concerning consciousness, its evolution, and the contributions made by psychology, philosophy and neurobiology.

Earthworm Protease

Directory of Open Access Journals (Sweden)

Rong Pan

2010-01-01

Full Text Available The alimentary tract of earthworm secretes a group of proteases with a relative wide substrate specificity. In 1983, six isozymes were isolated from earthworm with fibrinolytic activities and called fibriniolytic enzymes. So far, more isozymes have been found from different earthworm species such as Lumbricus rubellus and Eisenia fetida. For convenience, the proteases are named on the basis of the earthworm species and the protein function, for instance, Eisenia fetida protease (EfP. The proteases have the abilities not only to hydrolyze fibrin and other protein, but also activate proenzymes such as plasminogen and prothrombin. In the light of recent studies, eight of the EfPs contain oligosaccharides chains which are thought to support the enzyme structure. Interestingly, EfP-II has a broader substrate specificity presenting alkaline trypsin, chymotrypsin and elastase activities, but EfP-III-1 has a stricter specificity. The protein crystal structures show the characteristics in their specificities. Earthworm proteases have been applied in several areas such as clinical treatment of clotting diseases, anti-tumor study, environmental protection and nutritional production. The current clinical utilizations and some potential new applications of the earthworm protease will be discussed in this paper.

Effects of petroleum and metal contaminated soil on plants and earthworms: Survival and bioaccumulation

International Nuclear Information System (INIS)

Tatem, H.E.; Simmers, J.W.; Skogerboe, J.G.; Lee, C.R.

1993-01-01

Earthworms, Eisenia foetida, and bermudagrass, Cynodon dactylon, were used in the laboratory to test the toxicity of contaminated sediment taken from a small fresh water lake in North Carolina. This work was part of an investigation to determine the potential effects of upland disposal of this sediment. The contaminated sediment contained As, Cr, Cu, Pb, Hg, Ni, Zn and petroleum hydrocarbons at concentrations much greater than nearby soils. Test cylinders were planted with bermudagrass; earthworms were added 30 days later. Both species were harvested at 60 days, weighed and submitted for chemical analyses. Cynodon was affected by the contaminated sediment but grew well in the mixtures of sediment and upland soil. Similar results were obtained with the Eisenia. These species did not accumulate hydrocarbons from the sediment with the possible exception of pyrene. The metals Cd, Pb, and Zn were elevated in plants exposed to the contaminated sediment. Earthworms exposed to this sediment accumulated Pb to concentrations greater than animals exposed to the manure control. This work demonstrated that a contaminated freshwater sediment was not toxic to plants or earthworms and that most petroleum hydrocarbons were not accumulated. The only metal that may be of some concern was Pb

Relative proportions of polycyclic aromatic hydrocarbons differ between accumulation bioassays and chemical methods to predict bioavailability

Energy Technology Data Exchange (ETDEWEB)

Gomez-Eyles, Jose L., E-mail: j.l.gomezeyles@reading.ac.u [University of Reading, School of Human and Environmental Sciences, Department of Soil Science, Reading RG6 6DW, Berkshire (United Kingdom); Collins, Chris D.; Hodson, Mark E. [University of Reading, School of Human and Environmental Sciences, Department of Soil Science, Reading RG6 6DW, Berkshire (United Kingdom)

2010-01-15

Chemical methods to predict the bioavailable fraction of organic contaminants are usually validated in the literature by comparison with established bioassays. A soil spiked with polycyclic aromatic hydrocarbons (PAHs) was aged over six months and subjected to butanol, cyclodextrin and tenax extractions as well as an exhaustive extraction to determine total PAH concentrations at several time points. Earthworm (Eisenia fetida) and rye grass root (Lolium multiflorum) accumulation bioassays were conducted in parallel. Butanol extractions gave the best relationship with earthworm accumulation (r{sup 2} <= 0.54, p <= 0.01); cyclodextrin, butanol and acetone-hexane extractions all gave good predictions of accumulation in rye grass roots (r{sup 2} <= 0.86, p <= 0.01). However, the profile of the PAHs extracted by the different chemical methods was significantly different (p < 0.01) to that accumulated in the organisms. Biota accumulated a higher proportion of the heavier 4-ringed PAHs. It is concluded that bioaccumulation is a complex process that cannot be predicted by measuring the bioavailable fraction alone. - The ability of chemical methods to predict PAH accumulation in Eisenia fetida and Lolium multiflorum was hindered by the varied metabolic fate of the different PAHs within the organisms.

Earthworm Protease

International Nuclear Information System (INIS)

Pan, R.; Zhang, Z.; He, R.

2010-01-01

The alimentary tract of earthworm secretes a group of proteases with a relative wide substrate specificity. In 1983, six isozymes were isolated from earthworm with fibrinolytic activities and called fibrinolytic enzymes. So far, more isozymes have been found from different earthworm species such as Lumbricus rubellus and Eisenia fetida. For convenience, the proteases are named on the basis of the earthworm species and the protein function, for instance, Eisenia fetida protease (EfP). The proteases have the abilities not only to hydrolyze fibrin and other protein, but also activate pro enzymes such as plasminogen and prothrombin. In the light of recent studies, eight of the EfPs contain oligosaccharides chains which are thought to support the enzyme structure. Interestingly, EfP-II has a broader substrate specificity presenting alkaline trypsin, chymotrypsin and elastase activities, but EfP-III-1 has a stricter specificity. The protein crystal structures show the characteristics in their specificities. Earthworm proteases have been applied in several areas such as clinical treatment of clotting diseases, anti-tumor study, environmental protection and nutritional production. The current clinical utilizations and some potential new applications of the earthworm protease will be discussed in this paper.

Assessing soil ecotoxicity of methyl tert-butyl ether using earthworm bioassay; closed soil microcosm test for volatile organic compounds

International Nuclear Information System (INIS)

An, Youn-Joo

2005-01-01

An earthworm bioassay was conducted to assess ecotoxicity in methyl tert-butyl ether (MTBE)-amended soils. Ecotoxicity of MTBE to earthworms was evaluated by a paper contact method, natural field soil test, and an OECD artificial soil test. All tests were conducted in closed systems to prevent volatilization of MTBE out of test units. Test earthworm species were Perionyx excavatus and Eisenia andrei. Mortality and abnormal morphology of earthworms exposed to different concentrations of MTBE were examined. MTBE was toxic to both earthworm species and the severity of response increased with increasing MTBE concentrations. Perionyx excavatus was more sensitive to MTBE than Eisenia andrei in filter papers and two different types of soils. MTBE toxicity was more severe in OECD artificial soils than in field soils, possibly due to the burrowing behavior of earthworms into artificial soils. The present study demonstrated that ecotoxicity of volatile organic compounds such as MTBE can be assessed using an earthworm bioassay in closed soil microcosm with short-term exposure duration. - Earthworm bioassay can be a good protocol to assess soil ecotoxicity of volatile organic compounds such as MTBE

Venezuela’s Hugo Chavez, Bolivarian Socialism, and Asymmetric Warfare

Science.gov (United States)

2005-10-01

Francisco Rojas Aravena, “Nuevo contexto de seguridad internacional: nuevos desafios, nuevas oportunidades?” in La seguridad en America Latina pos 11...Institute, 2005; and Andres Benavente Urbina and Julio Alberto Cirino, “El populismo Chavista en Venezuela,” in La democracia defraudada, Buenos Aires... seguridad en Las Américas: Una mirada a la Conferencia Especial de Seguridad ,” Foro, Noviembre 2003, pp. 10–15. 20. Alvaro Vargas Llosa, “The

The use of soil quality indicators to assess soil functionality in restored semi-arid ecosystems

Science.gov (United States)

Muñoz-Rojas, Miriam; Erickson, Todd E.; Dixon, Kingsley W.; Merritt, David J.

2016-04-01

Keywords: Pilbara, 1-day CO2 test, microbial activity, mine restoration, soil health, ecosystem services. Introduction Semi-arid and arid environments are highly vulnerable to land degradation and their restoration has commonly showed low rates of success (James et al., 2013). A systematic knowledge of soil functionality is critical to successful restoration of degraded ecosystems since approximately 80% of ecosystem services can be connected to soil functions. The assessment of soil functionality generally involves the evaluation of soil properties and processes as they relate to the ability of soil to function effectively as a component of a healthy ecosystem (Costantini et al., 2015) Using soil quality indicators may be a valuable approach to assess functionality of topsoil and novel substrates used in restoration (Muñoz-Rojas et al., 2014; 2015). A key soil chemical indicator is soil organic C, that has been widely used as an attribute of soil quality because of the many functions that it provides and supports (Willaarts et al., 2015). However, microbial indicators can be more sensitive to disturbances and could be a valuable addition in soil assessment studies in restoration programs. Here, we propose a set of soil quality indicators to assess the soil status in restored soils (topsoil and waste material) of semi-arid environments. The study was conducted during March 2015 in the Pilbara biogeographical region (northwestern Australia) at an iron ore mine site rehabilitated in 2011. Methods Soil samples were collected from two sub-areas with different soil materials used as growth media: topsoil retrieved from nearby stockpiles and a lateritic waste material utilised for its erosive stability and physical competence. An undisturbed natural shrub-grassland ecosystem dominated by Triodia spp. and Acacia spp. representative of the restored area was selected as the analogue reference site. Soil physicochemical analysis were undertaken according to standard methods

Characterization and remediation of Soil Contaminated with Explosives: Development of Practical Technologies

Science.gov (United States)

2001-07-06

typically used as a surfactant allowing an increased pollutant bioavailability in various bioremediation treatment processes. In the particular case... Bioremediation f. 2000, 4, 27-39. (25) Hawari, J.In Biodegradation ofNitroaromatic Compounds and Explosives; Spain, J. C., Hughes, J. B., Knackmuss, H.-J...terrestrial plants such as sunflower and sanfroin. Significant sublethal effects of RDX were observed on the reproduction of earthworm Eisenia

Cost minimization analysis at the Hospital Mexico in the Caja Costarricense de Seguro Social for the accommodation of patients with radiotherapy with linear accelerator: shelter vs transfers

International Nuclear Information System (INIS)

Cespedes Quiros, Yehudi; Pereira Barrantes, Edgar

2010-01-01

Patients with oncological pathology have attended daily in sessions for several weeks in the linear accelerator radiation therapy. Persons subject to this intervention have moved to the Hospital Mexico of the social security of Costa Rica, place that centralizes this treatment. The Caja Costarricense de Seguro Social (CCSS) has considered two options depending on the place of origin of users: bear the costs of commuting from the area of habitual residence, or place them temporarily in a shelter provided by the institution; the CCSS has chosen the first alternative. This research has consisted of a cost-minimization analysis between these two alternatives. The study population are patients with oncological pathology attending sessions of radiotherapy with linear accelerator at the Hospital Mexico in the first half of 2009 using ambulances of Cruz Roja Costarricense. The size of the study population is 107 persons, the total number of transferred recorded in the Fondo Rotatorio de Operaciones of Hospital Mexico is 998. Patients came from five regions and twenty areas of health according to the geographical division of the CCSS. The variables included have been: cost per day by way of transfer, which involves the distance to the radiotherapy center of Hospital Mexico and the cost of the rate at Cruz Roja ambulance per Km (326.86 colons, according to the agreement CCSS-Cruz Roja in June 2009) estimated daily cost of lodging in a shelter, and the reason daily cost of relocation / daily cost of shelter. The cost of shelter includes food and were used data from the company International Medical Suppliers S. A. as a basis for estimating the daily cost, the price fixed by the company was 65 dollars a day, and the conversion into colons exchange rate as established by the Banco Central de Costa Rica; dated April 30, 2010, the amount has been 33.542.60 colons. The reason for each area of health was given by the ratio of the average daily cost in colons by way of ambulance of

Factores relacionados con la satisfacción laboral de enfermería en un hospital médico-quirúrgico Factors related to nursing staff job satisfaction in a medical and surgery hospital

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Juan Máximo Molina Linde

2009-09-01

Full Text Available Objetivo: analizar el grado de satisfacción laboral de los profesionales de enfermería que trabajan en un hospital médico-quirúrgico y establecer los factores sociodemográficos y profesionales relacionados con la misma. Metodología: estudio transversal y descriptivo. Se seleccionaron aleatoriamente 75 profesionales de enfermería (total: 510 del Hospital Médico Quirúrgico de la Ciudad Sanitaria "Virgen de las Nieves", de Granada, España, se autoadministra un cuestionario anónimo, compuesto por el Font-Roja (mide la satisfacción laboral y variables socio-demográficas y laborales. Resultados: la edad media de los encuestados es de 42,9 años; 72% mujeres, 64,9% casados, 73,3% vinculados en propiedad, 58,7% son del bloque de hospitalización y 67,6% tiene turno rotatorio. El grado de satisfacción global del Font Roja resulta medio (69,92 ± 10,48, recorrido entre 24 y 120. La dimensión mejor valorada es la «relación interpersonal con los compañeros» (3,82 ± 0,86 y la peor valorada la «promoción profesional» (2,28 ± 0,75. El bloque o lugar de trabajo es un elemento predictor de la satisfacción laboral (ß=-0,297, p=0,018. Análisis y discusión: un entorno de trabajo que garantice más satisfacción laboral para los profesionales de enfermería redundaría en mejoras de calidad en los cuidados recibidos por los pacientes de parte de este tipo de personal.Objective: to analyze the degree of job satisfaction of staff nurses working in a medical and surgical hospital, and to establish the socio-demographics and professional factors related to their job satisfaction. Methodology: cross-sectional and performed on seventy five persons of the nursing staff of the medical and surgery hospital (total: 510 of Ciudad Sanitaria Virgen de las Nieves of Granada (Spain, by means of a self-administered and anonymous survey. The questionnaire has two parts: the first one is Font-Roja test (it measures the job satisfaction and the second one

Cuando un príncipe besa a una rana: ¡Sorpresa! Una propuesta didáctica desde la homosexualidad

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José Antonio Jódar Sánchez

2017-11-01

Full Text Available Resumen: En esta propuesta didáctica, utilizamos el álbum ilustrado como herramienta para la enseñanza de contenido lingüístico, así como valores sociales y cívicos a un grupo de estudiantes de ELE de nivel A2. Utilizamos el álbum ilustrado El día de la rana roja que narra la historia de dos príncipes que se enamoran y adoptan a una niña huérfana. Estructuramos nuestra propuesta en cuatro fases (introducción, comprensión, consolidación, resignificación, de las cuáles las dos últimas son las dos que más desarrollamos. Planteamos una entrevista a la rana del cuento, un teatro con marionetas, y el dibujo del mapa del reino como actividades de introducción. En la fase de comprensión se sugieren actividades de léxico y gramática sencillas, tratando, por ejemplo, los tiempos de presente y futuro. En la fase de consolidación, el profesor tiene la opción de realizar el taller del adivinador de sueños o la puesta en escena de declaraciones de amor entre animales. Finalmente, para la fase de resignificación, se cuestiona por qué el rey y la reina cambian el vestido por unos pantalones al averiguar que su hijo se casará con un chico. Palabras clave: Homosexualidad, español como lengua extranjera, álbum ilustrado, El día de la rana roja.   Abstract: This teaching material is based on the picture book El día de la rana roja, the story of a prince who marries another man and together adopt an orphan child. It is targeted to students of Spanish as a foreign language of any country, including the United States, with an A2 level. Picture books are useful tools to teach, not only language and grammar, but also civility and good manners in society. The proposal is divided into four phases (introduction, comprehension, consolidation, and intellectual challenge, the latter of which are explained in detail. For the introduction phase, we propose interviewing the frog in the story, having a puppet role play narrating the story, and

Distribución y abundancia anual de Ceratium dens (Peridinales: Ceratiaceae en el golfo de California, México

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Roberto Cortés Altamirano

2000-06-01

Full Text Available La distribución de Ceratium dens en el Golfo de California se baso en tres campañas oceanográficas, su abundancia mensual (1995-96 en la Bahía de Mazatlán, y en tres fenómenos de mareas rojas presentadas en esta misma área. Se analizaron 52 muestras de fitoplancton recolectadas con red bongo con luz de malla de 64 µm y 120 muestras de agua con botella van Dorn en dos estaciones de recolecta y de tres eventos de marea roja en la Bahía de Mazatlán. Todas las muestras fueron contadas por el método del microscopio invertido. Los resultados indican que C. dens está presente en la región alta del Golfo de California y alrededor de Cabo San Lucas. En el ciclo 1995-96 la variación en la Bahía mostró dos períodos de máxima abundancia en primavera y otoño. Las abundancias presentaron una gran variación, en mar abierto fueron de 1-14 céls. l-1, en zonas costeras de 20-360 céls. l-1, y durante periodos de marea roja en 1985, 1989 y 1997 sus abundancias extremas fueron 144 000-256 000 céls. l-1, 100 000-400 000 céls. l-1 y 189 000-592 000 céls. l-1, respectivamente. Durante la marea roja, C. dens varía grandemente, a veces sustituida por Skeletonema costatum o Pseudonitzchia spp y Ceratium furca.. C. dens, aparenta tener una afinidad por los ambientes de alta productividad.Ceratium dens distribution in the Gulf of California was based on three oceanographic campaigns, monthly abundance (1995-96 and during three red tide events in Mazatlán Bay. 52 phytoplankton samples with a Bongo net and 64 µm mesh light, during the year 120 water samples with a van Dorn bottle were collected in two sampling stations and from three red tide events. All samples were counted by the inverted-microscope method. The results showed that C. dens was present in the upper Gulf of California and surroundings of Cabo San Lucas. Two peaks were observed during spring and autumn in Mazatlán Bay during 1995-1996. The highest densities (20-360 cells. . l-1 were

Dilong: Food for Thought and Medicine

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Edwin L. Cooper

2012-10-01

Full Text Available Earthworms have several names in different countries (In Chinese: 地龍 dì lóng, Japanese: Mimizu, Korean: Jireongi, Spanish: Lombriz de tierra, French: Ver de terre, German: Regenwurm, Italian: Lombrico, Swedish: Daggmask, Portuguese: Minhoca. They have long been used as a food source as well as treatments of various ailments. Many alternative and traditional disciplines of medicine, such as those in China, Japan, and Korea, developed medicinal uses of dilong from an initial utilization as nutrition. Increased curiosity in the potential medicinal properties of dilong has come to fruition through bioprospecting and evidence based research. This increased questioning and searching spawned first from a growing knowledge base about the earthworm's innate immune system. Their importance in understanding the evolution of the innate immune system has long been overlooked because of the ecological importance in soil preservation, earthworm immune systems, being full of leukocytes and humoral products, offer significant advantages when used as medicines. Earthworms offer an unanticipated slew of potential health benefits without common drawbacks that come with other biological, alternative forms of medicine such as cost, ethical and pathological concerns of animal testing.

Salida de campo al Sotillo (Valladolid) el 5 de febrero de 1952

OpenAIRE

Valverde Gómez, José Antonio, 1926-2003

2008-01-01

Salida de campo al "Sotillo", en Valladolid capital, el 5 de febrero de 1952, de la que se anotaron observaciones sobre las siguientes aves: Accipiter nisus (Gavilán común, también llamado Astur palumbarius por el autor), Aegithalos caudatus (Mito), Alectoris rufa (Perdiz roja), Cettia cetti (Ruiseñor bastardo), Corvus frugilegus (Graja), Corvus monedula (Grajilla, llamada Coloeus por el autor), Cyanistes caeruleus (Herrerillo común, llamado Parus coeruleus por el autor), Emberiza cirlus (Es...

Bibliography of Venomous and Poisonous Marine Animals and Their Toxins

Science.gov (United States)

1984-02-01

1972. (Paris) 105, 187, 1978. 479 ANON. Red tide research flows on. 488 AVARIA, S. Red tides off the coast BioScience 26, 223, 1976. of Chile . In...and CAMPOD6NICO, I. vol. 1, p. 157, 1965. Mareas rojas en Chile . Intercien- cia 3, 144, 1978. 735 HALSTEAD, B.W. Poisonous and Venomous Marine...hemolytic toxin from Chir- Aguas Brasileiras. Mem. Inst. onex fleckeri (box jelly-.ish). Butantan Simp. Iterrac. L3, 27, Proc. Aust. biochem. Soc. 9

La ciclooxigenasa-2 (COX-2) y el factor de crecimiento epidérmico (EFG) en lesiones epiteliales orales premalignas

OpenAIRE

Díaz Prado, S.; Gallego Guadalupe, A.; López-Cedrún, J.L.; Ferreras Granado, J.; Antón Aparicio, L.

2009-01-01

Las lesiones premalignas orales incluyen eritroplasias (manchas rojas) y leucoplasias (manchas blancas), las cuales se desarrollan a lo largo de superficies epiteliales. Estas lesiones son considerados marcadores en la "carcinogénesis de campo" ya que pacientes con lesiones premalignas orales pueden desarrollar carcinoma de células escamosas (CCS) en el sitio de las lesiones, así como en otros lugares de tracto aerodigestivo superior. Se está haciendo un gran esfuerzo para identificar nuevos ...

La ciclooxigenasa-2 (COX-2) y el factor de crecimiento epidérmico (EFG) en lesiones epiteliales orales premalignas Cyclooxygenase-2 (COX-2) and epidermal growth factor (EGF) in oral premalignant epithelial lesions

OpenAIRE

S. Díaz Prado; A. Gallego Guadalupe; J.L. López-Cedrún; J. Ferreras Granado; L. Antón Aparicio

2009-01-01

Las lesiones premalignas orales incluyen eritroplasias (manchas rojas) y leucoplasias (manchas blancas), las cuales se desarrollan a lo largo de superficies epiteliales. Estas lesiones son considerados marcadores en la "carcinogénesis de campo" ya que pacientes con lesiones premalignas orales pueden desarrollar carcinoma de células escamosas (CCS) en el sitio de las lesiones, así como en otros lugares de tracto aerodigestivo superior. Se está haciendo un gran esfuerzo para identificar nuevos ...

Comparaci??n de las propiedades antioxidantes y contenido de polifenoles de extractos acuosos de las algas marinas Bryothamnion triquetrum y Halimeda opuntia

OpenAIRE

D??az Gutierrez, Dayl??n; M??ndez Ortega, Wendy; Oliveira e Silva, A.M.; Zald??var Mu??oz, C.; Mancini-Filho, J.; Vidal-Novoa, A.

2015-01-01

Objetivos. Evaluar y comparar las propiedades antioxidantes mediante ensayos in vitro de extractos acuosos de las algas roja Bryothamnion triquetrum y verde Halimeda opuntia y su relaci??n con el contenido de polifenoles. Material y M??todos. Se utilizaron las t??cnicas in vitro: DPPH, Capacidad reductora, Inhibici??n de la peroxidaci??n lip??dica e inhibici??n de la hem??lisis inducida por AAPH. Resultados. B. triquetrum: DPPH; CI50=1,15 ?? 0,06, capacidad reductora a concentr...

Sowing of Organophonic Crops as a Teacher Praxis Centralized in Motivational Learning Strategies

OpenAIRE

Pedro Jacobo Caldera Tovar

2017-01-01

This study is aimed at teachers of the Aristides Rojas Educational Unit to identify the causes and consequences of school absenteeism, this problematic situation is evident in the records of control carried out daily and must be attended due to the rise of student absenteeism. The tutor, level coordinator, social pedagogy, student welfare and representatives must activate a plan in order to comply with the current legal regulations. School absenteeism affects the quality of student learning r...

Dicty_cDB: Contig-U10497-1 [Dicty_cDB

Lifescience Database Archive (English)

Full Text Available ce 9 from Patent WO0234921. ... 79 2e-13 AF511570_1( AF511570 |pid:none) Uncultured soil bacterium clone 57....000542_2092( CP000542 |pid:none) Verminephrobacter eiseniae EF01... 58 4e-07 AF511571_1( AF511571 |pid:none) Uncultured soil... Pseudomonas fluorescens Pf0-1, ... 51 7e-05 AF511572_1( AF511572 |pid:none) Uncultured soil bacterium clone

Aproximación a la vida y obra del pintor y estofador alcalaíno-granadino Pedro Raxis

Directory of Open Access Journals (Sweden)

Gila Medina, Lázaro

2003-12-01

Full Text Available This article offers an approximation to the life and work of Pedro de Raxis, a key artist in Counter Reformation Granada. Raxis was the nephew of the sculptor Pablo de Rojas, who was also the master of Juan Martinez Montañés. All three were from Alcalá de Henares and pioneers in the stylistic transition from the Roman Renaissance to early Baroque naturalism. Raxis also established an extremely active studio, precursor in the formation of famous artists, and during his lifetime was considered to be the "father" of the estofa technique (the sizing, gilding and painting of wooden statuary, although his facet as a painter was underestimated.

En el presente trabajo realizamos una aproximación a la vida y la obra de un artista clave para la Granada de la Contrarreforma: Pedro de Raxis, sobrino carnal del escultor Pablo de Rojas -maestro a su vez del también alcalaíno Juan Martínez Montañés-, todos ellos figuras pioneras al enmarcar el paso del renacimiento romanista al primer naturalismo barroco. Creador, igualmente, de un activísimo taller, precursor en la formación de renombrados artistas, fue considerado en su época como el "padre de la estofa", aunque se infravaloró su faceta de pintor.

Agronomic performance of quinoa selected in the Brazilian Savannah Desempenho agronômico de quinoa selecionada no Cerrado brasileiro

Directory of Open Access Journals (Sweden)

Carlos Roberto Spehar

2005-06-01

Full Text Available Twenty six breeding lines, selected from individual plant progenies of hybrids among varieties Amarilla de Marangani, Blanca de Junín, Chewecca, Faro 4, Improved Baer, Kancolla, Real, and Salares-Roja, had their agronomic characters evaluated, in Planaltina, DF, Brazil (15º36'S and 47º12'W, 1,000 masl, in randomized complete blocks, on a Ferralsol, previously limed and fertilized. Grain yield was positively associated with plant height, inflorescence length and diameter, and plant cycle. Genetic gain can be attained by selection based in these characters for commercial production of quinoa in tropical regions.Vinte e seis linhagens, selecionadas em progênies de plantas individuais de híbridos entre as variedades Amarilla de Marangani, Blanca de Junín, Chewecca, Faro 4, Improved Baer, Kancolla, Real e Salares-Roja, foram avaliadas quanto ao desempenho agronômico, em Planaltina, DF, Brasil (15º36'S e 47º12'W, 1.000 m de altitude, em delineamento de blocos ao acaso, em um Latossolo Vermelho-Escuro, previamente corrigido e adubado. O rendimento foi associado positivamente com altura de plantas, comprimento e diâmetro da inflorescência e ciclo da planta. Ganho genético pode ser obtido na seleção baseada nessas características, para o cultivo comercial da quinoa em regiões tropicais.

A new earthworm cellulase and its possible role in the innate immunity.

Science.gov (United States)

Park, In Yong; Cha, Ju Roung; Ok, Suk-Mi; Shin, Chuog; Kim, Jin-Se; Kwak, Hee-Jin; Yu, Yun-Sang; Kim, Yu-Kyung; Medina, Brenda; Cho, Sung-Jin; Park, Soon Cheol

2017-02-01

A new endogenous cellulase (Ean-EG) from the earthworm, Eisenia andrei and its expression pattern are demonstrated. Based on a deduced amino acid sequence, the open reading frame (ORF) of Ean-EG consisted of 1368 bps corresponding to a polypeptide of 456 amino acid residues in which is contained the conserved region specific to GHF9 that has the essential amino acid residues for enzyme activity. In multiple alignments and phylogenetic analysis, the deduced amino acid sequence of Ean- EG showed the highest sequence similarity (about 79%) to that of an annelid (Pheretima hilgendorfi) and could be clustered together with other GHF9 cellulases, indicating that Ean-EG could be categorized as a member of the GHF9 to which most animal cellulases belong. The histological expression pattern of Ean-EG mRNA using in situ hybridization revealed that the most distinct expression was observed in epithelial cells with positive hybridization signal in epidermis, chloragogen tissue cells, coelomic cell-aggregate, and even blood vessel, which could strongly support the fact that at least in the earthworm, Eisenia andrei, cellulase function must not be limited to digestive process but be possibly extended to the innate immunity. Copyright © 2016 Elsevier Ltd. All rights reserved.

Changes in bacterial numbers and microbial activity of pig slurry during gut transit of epigeic and anecic earthworms.

Science.gov (United States)

Aira, Manuel; Monroy, Fernando; Domínguez, Jorge

2009-03-15

In soils, organic matter decomposition and stabilization largely occur as a result of microbial activity, although when present, earthworms are important drivers of the processes through their interactions with microflora which begin during organic matter digestion by earthworms. Here, we studied the effects of gut transit on the number of bacteria and the microbial activity in pig slurry, using three epigeic (Eisenia fetida, Eisenia andrei, Eudrilus eugeniae) and one anecic (Octodrilus complanatus) species of earthworm. Bacterial counts revealed that the effect of gut transit on microbes differed depending on the earthworm species. Thus, no changes in the number of bacteria were found in the gut contents of E. fetida and E. eugeniae, whereas large decreases were recorded in those of O. complanatus and E. andrei (2.7 and 1.3 times, respectively). We suggest that, unlike in the three epigeic earthworm species, microorganisms are preferentially utilized by O. complanatus to meet its nutrient requirements, because of its limited digestive capacity. Despite the decrease in bacterial numbers, there were no differences in the gut contents of the four earthworm species or undigested pig slurry in terms of dehydrogenase activity. Therefore, we suggest that after gut transit in the four earthworm species under study the potential microbial degradation of pig slurry remains unaltered.

Fórmulas por mutuo acuerdo: Veintiún años continuos de dictaduras militar y bipartidista en colombia, 1953-1974

OpenAIRE

Mario Alexander Lozano García

2014-01-01

This paper sets out the customary actions regarding power struggle that political élites and the Colombian bourgeoisie undertook between the 50’s and 60’s in order to restore the position they held before General Gustavo Rojas Pinilla’s coup d’état and subsequent military dictatorship. For that, a common agreement known as Frente Nacional (National Front) was created. It was a “peace granting” formula which, in theory, articulated dictatorship terms and consociationalism; these qualitative va...

"Pistolas para el Niño, Muñecas para la Niña"

OpenAIRE

Gianini Belotti, Elena

2004-01-01

Educere:Una Brizna de Aliento en el Tiempo Rivas, Pedro El Paradigma Reinante y su Paideia Alonso, Leonor La Experiencia Contemporánea como Expresión del Fin de una ÉticaAlgunas Ideas desde la Postmodernidad para Releer el Dato Educativo Perdomo, José Camilo La Sociedad del Conocimiento y el Fin de la Escuela Rodríguez Rojas, Pedro Escuela, Alteridad y Experiencia de SíLa Producción Pedagógica del Sujeto Valera Villegas, G...

La Experiencia Contemporánea como Expresión del Fin de una Ética

OpenAIRE

Perdomo, José Camilo

2004-01-01

Educere:Una Brizna de Aliento en el Tiempo Rivas, Pedro El Paradigma Reinante y su Paideia Alonso, Leonor La Experiencia Contemporánea como Expresión del Fin de una ÉticaAlgunas Ideas desde la Postmodernidad para Releer el Dato Educativo Perdomo, José Camilo La Sociedad del Conocimiento y el Fin de la Escuela Rodríguez Rojas, Pedro Escuela, Alteridad y Experiencia de SíLa Producción Pedagógica del Sujeto Valera Villegas, G...

Algunos Obstáculos Conceptuales y Metodológicos Observados en el Diseño y Desarrollo de los Proyectos Pedagógicos de Aula

OpenAIRE

Rivas, Pedro José

2004-01-01

Educere:Una Brizna de Aliento en el Tiempo Rivas, Pedro El Paradigma Reinante y su Paideia Alonso, Leonor La Experiencia Contemporánea como Expresión del Fin de una ÉticaAlgunas Ideas desde la Postmodernidad para Releer el Dato Educativo Perdomo, José Camilo La Sociedad del Conocimiento y el Fin de la Escuela Rodríguez Rojas, Pedro Escuela, Alteridad y Experiencia de SíLa Producción Pedagógica del Sujeto Valera Villegas, G...

La Sociedad del Conocimiento y el Fin de la Escuela

OpenAIRE

Rodríguez Rojas, Pedro

2004-01-01

Educere:Una Brizna de Aliento en el Tiempo Rivas, Pedro El Paradigma Reinante y su Paideia Alonso, Leonor La Experiencia Contemporánea como Expresión del Fin de una ÉticaAlgunas Ideas desde la Postmodernidad para Releer el Dato Educativo Perdomo, José Camilo La Sociedad del Conocimiento y el Fin de la Escuela Rodríguez Rojas, Pedro Escuela, Alteridad y Experiencia de SíLa Producción Pedagógica del Sujeto Valera Villegas, G...

Desarrollo del Potencial Humano en el Docente:

OpenAIRE

Escobar Domínguez, María Gisela

2004-01-01

Educere:Una Brizna de Aliento en el Tiempo Rivas, Pedro El Paradigma Reinante y su Paideia Alonso, Leonor La Experiencia Contemporánea como Expresión del Fin de una ÉticaAlgunas Ideas desde la Postmodernidad para Releer el Dato Educativo Perdomo, José Camilo La Sociedad del Conocimiento y el Fin de la Escuela Rodríguez Rojas, Pedro Escuela, Alteridad y Experiencia de SíLa Producción Pedagógica del Sujeto Valera Villegas, G...

Desarrollo Corporal en Niños Preescolares del Municipio Autónomo Libertador del Estado Mérida

OpenAIRE

Prado P., José Rafael; Meza C., Yasmín L.

2004-01-01

Educere:Una Brizna de Aliento en el Tiempo Rivas, Pedro El Paradigma Reinante y su Paideia Alonso, Leonor La Experiencia Contemporánea como Expresión del Fin de una ÉticaAlgunas Ideas desde la Postmodernidad para Releer el Dato Educativo Perdomo, José Camilo La Sociedad del Conocimiento y el Fin de la Escuela Rodríguez Rojas, Pedro Escuela, Alteridad y Experiencia de SíLa Producción Pedagógica del Sujeto Valera Villegas, G...

Evaluación de la degradabilidad in situ en bovinos suplementados con cuatro especies arbóreas

OpenAIRE

Roa V, María; Muñoz M, Javier

2012-01-01

Objetivo. Evaluar degradabilidad in situ en rumen de cuatro especies forrajeras: Acacia Roja (Delonix regia), pízamo (Eritryna glauca), Cratilia (Cratylia argentea) y casco de vaca (Bahuinia variegata), para determinar su calidad nutricional. Materiales y métodos. Cuatro hembras rumino-fistuladas en un diseño de sobrecambio simple, pastoreando en Brachiaria decumbens, suplementadas en la mañana con tres kg de hojas deshidratadas de las cuatro especies mencionadas, de un año de establecidas y ...

Impacto Ambiental y Económico de la Liberalización de Maíz Genéticamente Modificado (GM) en Chile

OpenAIRE

Osorio, José Díaz; Jara-Rojas, Roberto; Moya, Francisco

2012-01-01

J. Díaz Osorio, R. Jara-Rojas and F. Moya. 2012. Environmental and economic impact to the liberalization of genetic modified maize in Chile. Currently, about 125 million of hectares of GM crops are grown in 25 countries. Some authors establish that GM crops have contributed to reduce the environmental impact of agriculture and reduce the use of machinery and chemicals. In Chile, seed production of GM crops is only allowed for export purposes; however, it cannot be used as input for commercial...

Development of Nanostructure Materials and Architecture for High Performance Li-rechargeable Batteries with Ultrafast Charge Rate

Science.gov (United States)

2011-04-22

overcome these difficulties extensive work has been done. Surface coatings with ZnO , SiO2, and Bi2O3 have been proposed to enhance stability of electrodes...Liu, G. Q.; Wen, L.; Liu, Y. M. Journal of Solid State Electrochemistry 2010, 14, 2191. (23) Myung, S. T.; Komaba, S.; Kumagai, N.; Yashiro, H.; Chung...Y. R.; Shu, J.; Zhu, R. S. Journal of Solid State Electrochemistry 2009, 13, 913. (29) Aklalouch, M.; Amarilla, J. M.; Rojas, R. M.; Saadoune, I

The Spanish Emergency Military Unit: Military Capabilities in a Civilian Environment

Science.gov (United States)

2015-05-23

of Weapons of Mass Destruction X X X Irregular Immigration X X X Espionage X Disasters and Emergencies X X X Maritime Vulnerability X...needs. The magnitude of the disaster, and the mishandling of the initial response, forced the governor of Florida, Mr. Lawton Chiles , to request for...Cruz Roja en el envío de ayuda a Haití y Chile .” 6 October 2010. Accessed 3 December 2014. http://www.defensa.gob.es/ gabinete/notasPrensa/2010/06

Epigeic Earthworms Exert a Bottleneck Effect on Microbial Communities through Gut Associated Processes

OpenAIRE

Gómez-Brandón, María; Aira, Manuel; Lores, Marta; Domínguez, Jorge

2011-01-01

BACKGROUND: Earthworms play a critical role in organic matter decomposition because of the interactions they establish with microorganisms. The ingestion, digestion, assimilation of organic material in the gut and then casting is the first step in earthworm-microorganism interactions. The current knowledge of these direct effects is still limited for epigeic earthworm species, mainly those living in man-made environments. Here we tested whether and to what extent the earthworm Eisenia andrei ...

Representaciones animales y procesos de borramiento en el conflicto Cresta Roja

Directory of Open Access Journals (Sweden)

Diego Luis Forte

2017-06-01

Full Text Available At the end of 2015 the firm Rasic Brothers, one of the largest poultry producers in Argentina, paralyzes its production leaving all employees on the street with several months of salary in debt. These employees decide to establish a picket in the Ricchieri highway, awaiting for an answer from both the company and government. As the conflict develops, an enormous amount of animals are enclosed without food or water and the judge in charge of the case decides to take a month to resolve their situation. The aim of this paper is to analyze the construction of speciesist representations in a corpus of news published in digital media. Analysis will focus on two notes published in the website of the newspaper called Clarin, a note appeared in the website of the newspaper Página 12 and an editorial note published by the NGO Voicot on their Facebook page. We start from the hypothesis that the speciesist representations are so embedded in the mass media discourse that they do not generate a justification for their existence, as do other sort of prejudice, and they erase all evidence to consider nonhuman animals as living beings. Analysis will take the theoretical proposal of Critical Discourse Analysis, as main framework, adopting the perspective provided by Critical Linguistics (Hodge and Kress, 1993 for the assignment of thematic roles and description of processes in the verbal material, together with the elements systemized by Kress and van Leeuwen (1996 for image analysis and multimodal analysis.

Understanding environmental drivers in the regulation of soil respiration dynamics after fire in semi-arid ecosystems

Science.gov (United States)

Muñoz-Rojas, Miriam; Lewandrowski, Wolfgang; Erickson, Todd E.; Dixon, Kingsley W.; Merritt, David J.

2016-04-01

Keywords: Pilbara, soil CO2 efflux, soil C, soil moisture, soil temperature Introduction Soil respiration (Rs) has become a major research focus given the increase in atmospheric CO2 emissions and the large contribution of these CO2 fluxes from soils (Van Groenigen et al., 2014). In addition to its importance in the global C cycle, Rs is a fundamental indicator of soil health and quality that reflects the level of microbial activity and provides an indication of the ability of soils to support plant growth (Oyonarte et al., 2012; Munoz-Rojas et al., 2015). Wildfires can have a significant impact on Rs rates, with the scale of the impact depending on environmental factors such as temperature and moisture, and organic C content in the soil. Vegetation cover can have a significant effect on regulating organic C contents; and while advances are made into understanding the effects of fire on organic C contents and CO2 fluxes (Granged et al., 2011; Willaarts et al., 2015; Muñoz-Rojas et al., 2016), there is limited knowledge of the variability of Rs across ecosystem types, vegetation communities, and responses to fire. In this research we aimed to assess the impacts of a wildfire on the soil CO2 fluxes and soil respiration in a semi-arid ecosystem of Western Australia (Pilbara biogeographical region), and to understand the main environmental drivers controlling these fluxes in different vegetation types. The study has application for other arid and semi-arid regions of the world. Methods The study area was selected following a wildfire that affected 25 ha in February 2014. Twelve plots were established in the burnt site (B) within a 400 m2 area, and 12 plots in an adjacent unburnt control site. At each site, three plots were installed below the canopy of each of the most representative vegetation types of the areas: Eucalyptus trees, Acacia shrubs and Triodia grasses, and three on bare soil. Soil sampling and measurement of soil CO2 efflux, temperature and moisture were

Computation of the power spectrum in chaotic ¼λφ4 inflation

International Nuclear Information System (INIS)

Rojas, Clara; Villalba, Víctor M.

2012-01-01

The phase-integral approximation devised by Fröman and Fröman, is used for computing cosmological perturbations in the quartic chaotic inflationary model. The phase-integral formulas for the scalar power spectrum are explicitly obtained up to fifth order of the phase-integral approximation. As in previous reports (Rojas 2007b, 2007c and 2009), we point out that the accuracy of the phase-integral approximation compares favorably with the numerical results and those obtained using the slow-roll and uniform approximation methods

Computation of the power spectrum in chaotic ¼λφ{sup 4} inflation

Energy Technology Data Exchange (ETDEWEB)

Rojas, Clara [Centro de Estudios Interdisciplinarios de la Física, Instituto Venezolano de Investigaciones Científicas (IVIC), Carretera Panamericana Km. 11, Caracas 1020A (Venezuela, Bolivarian Republic of); Villalba, Víctor M., E-mail: clararoj@gmail.com, E-mail: Victor.Villalba@monash.edu [School of Mathematical Sciences, Faculty of Science, Monash University, Clayton, Vic 3800 (Australia)

2012-01-01

The phase-integral approximation devised by Fröman and Fröman, is used for computing cosmological perturbations in the quartic chaotic inflationary model. The phase-integral formulas for the scalar power spectrum are explicitly obtained up to fifth order of the phase-integral approximation. As in previous reports (Rojas 2007b, 2007c and 2009), we point out that the accuracy of the phase-integral approximation compares favorably with the numerical results and those obtained using the slow-roll and uniform approximation methods.

Estudio preliminar de la profilaxis de la epilepsia en acúmulos, con midazolam.

OpenAIRE

Paredes L., Gustavo A.; Andrade, M.; Vielma, Marly; Rondón, Juana

2006-01-01

Editorial. ¡Ya tenemos símbolo, ícono o logotipo!. Now we have symbol, icono or logo!. Salinas, Pedro José Accidentes domésticos en ancianos. Municipio Libertador. Mérida. 1993-1996. Domestics accidents in elderly people. Libertador County of Mérida State. 1993-1996. Salinas, Pedro José Rojas Márquez, Reina Estrés y síntomas en personal de salud del Hospital Universitario de Los Andes. Stress and symptoms in health staff of the Hospital Universitario de Los Andes. Méri...

Actividad de la arginasa sérica en pacientes con carcinoma gástrico.

OpenAIRE

Alarcón Corredor, Oscar Marino; Tauil B., Eduar; Ramírez de Fernandez, María; D'Jésus A., Iraima; Navarro, Fanny; Alfonzo, Reina; Carrero M., Pablo E.; Rondón, Carlos

2007-01-01

Editorial. ¡Ya tenemos símbolo, ícono o logotipo!. Now we have symbol, icono or logo!. Salinas, Pedro José Accidentes domésticos en ancianos. Municipio Libertador. Mérida. 1993-1996. Domestics accidents in elderly people. Libertador County of Mérida State. 1993-1996. Salinas, Pedro José Rojas Márquez, Reina Estrés y síntomas en personal de salud del Hospital Universitario de Los Andes. Stress and symptoms in health staff of the Hospital Universitario de Los Andes. Méri...

Tiroidectomia total en afecciones benignas de la glándula tiroides.

OpenAIRE

Lucena Olavarrieta, Jorge R.; Coronel, Paúl; Orellana, Ysabelen

2006-01-01

Editorial. ¡Ya tenemos símbolo, ícono o logotipo!. Now we have symbol, icono or logo!. Salinas, Pedro José Accidentes domésticos en ancianos. Municipio Libertador. Mérida. 1993-1996. Domestics accidents in elderly people. Libertador County of Mérida State. 1993-1996. Salinas, Pedro José Rojas Márquez, Reina Estrés y síntomas en personal de salud del Hospital Universitario de Los Andes. Stress and symptoms in health staff of the Hospital Universitario de Los Andes. Méri...

Accidentes domésticos en ancianos. Municipio Libertador. Mérida. 1993 - 1996.

OpenAIRE

Rojas Márquez, Reina; Salinas, Pedro José

2006-01-01

Editorial. ¡Ya tenemos símbolo, ícono o logotipo!. Now we have symbol, icono or logo!. Salinas, Pedro José Accidentes domésticos en ancianos. Municipio Libertador. Mérida. 1993-1996. Domestics accidents in elderly people. Libertador County of Mérida State. 1993-1996. Salinas, Pedro José Rojas Márquez, Reina Estrés y síntomas en personal de salud del Hospital Universitario de Los Andes. Stress and symptoms in health staff of the Hospital Universitario de Los Andes. Méri...

Ausentismo laboral de causa médica en el Instituto Autónomo Hospital Universitario de Los Andes.

OpenAIRE

Parada de Denis, María Eugenia; Rivas Padilla, Fernando; Moreno Barrios, Reyla; Rincón, Eglis; Mejía, Zurayma; Mora, Dania Margarita

2006-01-01

Editorial. ¡Ya tenemos símbolo, ícono o logotipo!. Now we have symbol, icono or logo!. Salinas, Pedro José Accidentes domésticos en ancianos. Municipio Libertador. Mérida. 1993-1996. Domestics accidents in elderly people. Libertador County of Mérida State. 1993-1996. Salinas, Pedro José Rojas Márquez, Reina Estrés y síntomas en personal de salud del Hospital Universitario de Los Andes. Stress and symptoms in health staff of the Hospital Universitario de Los Andes. Méri...

Editorial. ¡Ya tenemos símbolo, ícono o logotipo!

OpenAIRE

Salinas, Pedro José

2006-01-01

Editorial. ¡Ya tenemos símbolo, ícono o logotipo!. Now we have symbol, icono or logo!. Salinas, Pedro José Accidentes domésticos en ancianos. Municipio Libertador. Mérida. 1993-1996. Domestics accidents in elderly people. Libertador County of Mérida State. 1993-1996. Salinas, Pedro José Rojas Márquez, Reina Estrés y síntomas en personal de salud del Hospital Universitario de Los Andes. Stress and symptoms in health staff of the Hospital Universitario de Los Andes. Méri...

Estrés y síntomas en personal de salud del Hospital Universitario de Los Andes.

OpenAIRE

Manzanilla, María D.; Molina de González Méndez, Tivizay; Caltagirone, Raimondo; Vera , Mariflor; Torres, Adrián R.

2007-01-01

Editorial. ¡Ya tenemos símbolo, ícono o logotipo!. Now we have symbol, icono or logo!. Salinas, Pedro José Accidentes domésticos en ancianos. Municipio Libertador. Mérida. 1993-1996. Domestics accidents in elderly people. Libertador County of Mérida State. 1993-1996. Salinas, Pedro José Rojas Márquez, Reina Estrés y síntomas en personal de salud del Hospital Universitario de Los Andes. Stress and symptoms in health staff of the Hospital Universitario de Los Andes. Méri...

SELECTIVIDAD DEL INSECTICIDA CARTAP EMPLEANDO BIOENSAYOS CON ORGANISMOS NO DESTINATARIOS

OpenAIRE

Iannacone, José; Universidad Nacional Federico Villarreal (Perú).; Alvariño, Lorena; Universidad Nacional Federico Villarreal (Perú).

2016-01-01

El objetivo de la presente investigación fue evaluar la ecotoxicidad del cartap (Bala® 50 PS) sobre ocho organismos animales no destinatarios: 1) Melanoides tuberculata (Müller, 1774) (Mollusca), 2) Emerita analoga (Stimpson, 1857) (Crustácea), 3) Chironomus calligraphus Goeldi, 1805 (Diptera), 4) Cyprinus carpio (Linné, 1758) (Osteichyties), 5) Eisenia foetida (Savigny, 1826) (Annelida), 6) Podisus nigrispinus (Dallas, 1851) (Hemiptera), 7) Trichogramma fuentesi Torre, 1980 (Hymenoptera) y 8...

Del cinematógrafo a la televisión educativa: El uso estatal de las tecnologías de comunicación en Colombia (1935 - 1957.

Directory of Open Access Journals (Sweden)

Marcela Uribe Sánchez.

2004-07-01

Full Text Available Escasa en la literatura sobre la historia de la televisión en Colombia, es común encontrar la idea de que la, televisión educativa y cultural Fue un invento del general Rojas Pinilla, que respondía A LOS Intereses populistas de su gobierno. Sin embargo, el interés de este artículo es mostrar como este carácter respondía más bien a la Manera en que el Estado colombiano venia Utilizando los "nuevos" medios masivos de comunicación desde Mediados de la década de los años treinta del siglo XX. TENIENDO como horizonte la primera fase del Proceso de masificación en América Latina y desde una historia Qué tendrá como marco las relaciones entre comunicación y cultura, nos detendremos en las relaciones entre la experiencia del uso del cine en las campañas educativas Estatales desde 1935, y la de los primeros años de la televisión en Colombia (1954-1957 Bajo el gobierno de Gustavo Rojas Pinilla. Dicha relación se articulará en torno al uso del lenguaje audiovisual como herramienta educativa en las campañas Estatales que recorrieron el periodo, y que en últimas Dieron forma A UNA tradición estatal de uso de los nuevos medios.

Protective effect of the edible brown alga Ecklonia stolonifera on doxorubicin-induced hepatotoxicity in primary rat hepatocytes.

Science.gov (United States)

Jung, Hyun Ah; Kim, Jae-I; Choung, Se Young; Choi, Jae Sue

2014-08-01

As part of our efforts to isolate anti-hepatotoxic agents from marine natural products, we screened the ability of 14 edible varieties of Korean seaweed to protect against doxorubicin-induced hepatotoxicity in primary rat hepatocytes. Among the crude extracts of two Chlorophyta (Codium fragile and Capsosiphon fulvescens), seven Phaeophyta (Undaria pinnatifida, Sargassum thunbergii, Pelvetia siliquosa, Ishige okamurae, Ecklonia cava, Ecklonia stolonifera and Eisenia bicyclis), five Rhodophyta (Chondrus ocellatus, Gelidium amansii, Gracilaria verrucosa, Symphycladia latiuscula and Porphyra tenera), and the extracts of Ecklonia stolonifera, Ecklonia cava, Eisenia bicyclis and Pelvetia siliquosa exhibited significant protective effects on doxorubicin-induced hepatotoxicity, with half maximal effective concentration (EC50) values of 2.0, 2.5, 3.0 and 15.0 μg/ml, respectively. Since Ecklonia stolonifera exhibits a significant protective potential and is frequently used as foodstuff, we isolated six phlorotannins, including phloroglucinol (1), dioxinodehydroeckol (2), eckol (3), phlorofucofuroeckol A (4), dieckol (5) and triphloroethol-A (6). Phlorotannins 2 ∼ 6 exhibited potential protective effects on doxorubicin-induced hepatotoxicity, with corresponding EC50 values of 3.4, 8.3, 4.4, 5.5 and 11.5 μg/ml, respectively. The results clearly demonstrated that the anti-hepatotoxic effects of Ecklonia stolonifera and its isolated phlorotannins are useful for further exploration and development of therapeutic modalities for treatment of hepatotoxicity. © 2014 Royal Pharmaceutical Society.

Synergistic Effects of Sulfated Polysaccharides from Mexican Seaweeds against Measles Virus

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Karla Morán-Santibañez

2016-01-01

Full Text Available Sulfated polysaccharides (SPs extracted from five seaweed samples collected or cultivated in Mexico (Macrocystis pyrifera, Eisenia arborea, Pelvetia compressa, Ulva intestinalis, and Solieria filiformis were tested in this study in order to evaluate their effect on measles virus in vitro. All polysaccharides showed antiviral activity (as measured by the reduction of syncytia formation and low cytotoxicity (MTT assay at inhibitory concentrations. SPs from Eisenia arborea and Solieria filiformis showed the highest antiviral activities (confirmed by qPCR and were selected to determine their combined effect. Their synergistic effect was observed at low concentrations (0.0274 μg/mL and 0.011 μg/mL of E. arborea and S. filiformis SPs, resp., which exhibited by far a higher inhibitory effect (96% syncytia reduction in comparison to the individual SP effects (50% inhibition with 0.275 μg/mL and 0.985 μg/mL of E. arborea and S. filiformis, resp.. Time of addition experiments and viral penetration assays suggest that best activities of these SPs occur at different stages of infection. The synergistic effect would allow reducing the treatment dose and toxicity and minimizing or delaying the induction of antiviral resistance; sulfated polysaccharides of the tested seaweed species thus appear as promising candidates for the development of natural antiviral agents.