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Sample records for loft l2-5 porovnani

  1. Assessment of the RELAP5 multi-dimensional component model using data from LOFT test L2-5

    International Nuclear Information System (INIS)

    Davis, C.B.

    1998-01-01

    The capability of the RELAP5-3D computer code to perform multi-dimensional analysis of a pressurized water reactor (PWR) was assessed using data from the LOFT L2-5 experiment. The LOFT facility was a 50 MW PWR that was designed to simulate the response of a commercial PWR during a loss-of-coolant accident. Test L2-5 simulated a 200% double-ended cold leg break with an immediate primary coolant pump trip. A three-dimensional model of the LOFT reactor vessel was developed. Calculations of the LOFT L2-5 experiment were performed using the RELAP5-3D Version BF02 computer code. The calculated thermal-hydraulic responses of the LOFT primary and secondary coolant systems were generally in reasonable agreement with the test. The calculated results were also generally as good as or better than those obtained previously with RELAP/MOD3

  2. Experiment data report for LOFT large-break loss-of-coolant experiment L2-5

    International Nuclear Information System (INIS)

    Bayless, P.D.; Divine, J.M.

    1982-08-01

    Selected pertinent and uninterpreted data from the third nuclear large break loss-of-coolant experiment (Experiment L2-5) conducted in the Loss-of-Fluid Test (LOFT) facility are presented. The LOFT facility is a 50-MW(t) pressurized water reactor (PWR) system with instruments that measure and provide data on the system thermal-hydraulic and nuclear conditions. The operation of the LOFT system is typical of large [approx. 1000 MW(e)] commercial PWR operations. Experiment L2-5 simulated a double-ended offset shear of a cold leg in the primary coolant system. The primary coolant pumps were tripped within 1 s after the break initiation, simulating a loss of site power. Consistent with the loss of power, the starting of the high- and low-pressure injection systems was delayed. The peak fuel rod cladding temperature achieved was 1078 +- 13 K. The emergency core cooling system re-covered the core and quenched the cladding. No evidence of core damage was detected

  3. Best estimate prediction for LOFT nuclear experiment L3-2

    International Nuclear Information System (INIS)

    Kee, E.J.; Shinko, M.S.; Grush, W.H.; Condie, K.G.

    1980-02-01

    Comprehensive analyses using both the RELAP4 and the RELAP5 computer codes were performed to predict the LOFT transient thermal-hydraulic response for nuclear Loss-of-Coolant Experiment L3-2 to be performed in the Loss-of-Fluid Test (LOFT) facility. The LOFT experiment will simulate a small break in one of the cold legs of a large four-loop pressurized water reactor and will be conducted with the LOFT reactor operating at 50 MW. The break in LOCE L3-2 is sized to cause the break flow to be approximately equal to the high-pressure injection system flow at an intermediate pressure of approximately 7.6 MPa

  4. LOFT/L2-5, Loss of Fluid Test, 3. NRC L2 Large Break LOCA Experiment

    International Nuclear Information System (INIS)

    1992-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: This experiment was the third of the NRC L2 Series of nuclear large Break LOCA experiments, conducted on 16 June 1981. It simulated a 100% cold leg break with a maximum heat generation of 40 kW/m and rapid pump coast down

  5. LOFT/L3-5, Loss of Fluid Test, 5. NRC L3 Small Break LOCA Experiment

    International Nuclear Information System (INIS)

    1991-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: This was the fifth in the NRC L3 Series of small-break LOCA experiments. A 10-cm (2.5-in.) cold-leg non-communicative-break LOCA was simulated. Pumps were shut off. The experiment was conducted on 29 September 1980

  6. Experiment prediction for LOFT nuclear experiments L5-1/L8-2

    International Nuclear Information System (INIS)

    Chen, T.H.; Modro, S.M.

    1982-01-01

    The LOFT Experiments L5-1 and L8-2 simulated intermediate break loss-of-coolant accidents with core uncovery. This paper compares the predictions with the measured data for these experiments. The RELAP5 code was used to perform best estimate double-blind and single-blind predictions. The double-blind calculations are performed prior to the experiment and use specified nominal initial and boundary conditions. The single-blind calculations are performed after the experiment and use measured initial and boundary conditions while maintaining all other parameters constant, including the code version. Comparisons of calculated results with experimental results are discussed; the possible causes of discrepancies are explored and explained. RELAP5 calculated system pressure, mass inventory, and fuel cladding temperature agree reasonably well with the experiment results, and only slight changes are noted between the double-blind and single-blind predictions

  7. Experiment prediction for LOFT nuclear experiments L5-1 and L8-2

    International Nuclear Information System (INIS)

    Chen, T.H.; Modro, S.M.

    1983-01-01

    The LOFT Experiments L5-1 and L8-2 simulated intermediate break loss-of-coolant accidents with core uncovery. This paper compares the predictions with the measured data for these experiments. The RELAP5 code was used to perform best estimate double-blind and single-blind predictions. The double-blind calculations are performed prior to the experiment and use specified nominal initial and boundary conditions. The single-blind calculations are performed after the experiment and use measured initial and boundary conditions while maintaining all other parameters constant, including the code version. Comparisons of calculated results with experimental results are discussed; the possible causes of discrepancies are explored and explained. RELAP5 calculated system pressure, mass inventory, and fuel cladding temperature agree reasonably well with the experiment results, and only slight changes are noted between the double-blind and single-blind predictions

  8. Sensitivity analysis of LOFT L2-5 test calculations

    International Nuclear Information System (INIS)

    Prosek, Andrej

    2014-01-01

    The uncertainty quantification of best-estimate code predictions is typically accompanied by a sensitivity analysis, in which the influence of the individual contributors to uncertainty is determined. The objective of this study is to demonstrate the improved fast Fourier transform based method by signal mirroring (FFTBM-SM) for the sensitivity analysis. The sensitivity study was performed for the LOFT L2-5 test, which simulates the large break loss of coolant accident. There were 14 participants in the BEMUSE (Best Estimate Methods-Uncertainty and Sensitivity Evaluation) programme, each performing a reference calculation and 15 sensitivity runs of the LOFT L2-5 test. The important input parameters varied were break area, gap conductivity, fuel conductivity, decay power etc. For the influence of input parameters on the calculated results the FFTBM-SM was used. The only difference between FFTBM-SM and original FFTBM is that in the FFTBM-SM the signals are symmetrized to eliminate the edge effect (the so called edge is the difference between the first and last data point of one period of the signal) in calculating average amplitude. It is very important to eliminate unphysical contribution to the average amplitude, which is used as a figure of merit for input parameter influence on output parameters. The idea is to use reference calculation as 'experimental signal', 'sensitivity run' as 'calculated signal', and average amplitude as figure of merit for sensitivity instead for code accuracy. The larger is the average amplitude the larger is the influence of varied input parameter. The results show that with FFTBM-SM the analyst can get good picture of the contribution of the parameter variation to the results. They show when the input parameters are influential and how big is this influence. FFTBM-SM could be also used to quantify the influence of several parameter variations on the results. However, the influential parameters could not be

  9. LOFT/L2-3, Loss of Fluid Test, 2. NRC L2 Large Break LOCA Experiment

    International Nuclear Information System (INIS)

    1992-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: This experiment was the second of the NRC L2 Series of nuclear large Break LOCA experiments, and was conducted on 12 May 1979. It simulated a 100% cold leg break with a maximum heat generation of 39 kW/m

  10. Assessment of RELAP5/MOD3.1 using LOFT L2-3 experiment data

    International Nuclear Information System (INIS)

    Lee, Sang Yong; Ban, Chang Hwan; Chung, Bob Dong

    1994-06-01

    The capability of RELAP5/MOD3.1 to predict overall LOCA thermal hydraulic phenomena was assessed utilizing the data of LOFT L2-3 experiment. Loop behaviors such as mass flow rate, water density, momentum flux, and the heating-up and rewetting of the fuel rod cladding during blowdown were well calculated. Reflood heat-up of the fuel rod cladding at the high power region of the core was reasonably predicted. But in the upper part of the core, cladding heat-up was calculated incorrectly since present code has no capability to calculate the top-down quenching which of highly multi-dimensional behavior. (Author) 10 refs., 46 figs., 2 tabs

  11. Analysis of LOFT loss-of-coolant experiments L2-2, L2-3, and L3-0

    International Nuclear Information System (INIS)

    Leach, L.P.; Linebarger, J.H.

    1979-01-01

    A summary of results from Loss-of-Coolant Experiments (LOCE) L2-2, L2-3, and L3-0, conducted in the Loss-of-Fluid Test (LOFT) facility, and conclusions from posttest analyses of the experimental data are presented. LOCEs L2-2 and L2-3 were nuclear large break experiments and were dominated by a core-wide fuel rod cladding rewet, which limited the maximum fuel temperature. Analytical models only conservatively predicted the measured fuel rod temperatures and will require improvements to provide best estimate predictions in this area. Analysis of a large commercial pressurized water reactor (PWR) indicates that the cladding rewet observed in LOFT is also likely to occur in a large PWR, and that, therefore, safety analysis calculations of large loss-of-coolant accidents (LOCA) are more conservative than previously thought. LOCE L3-0 was an isothermal small break (top of pressurizer) experiment and illustrated that the pressurizer fills after the primary system fluid saturates someplace other than the pressurizer itself, that the indicated pressurizer level is higher than the actual level, and that additional model development and assessment work is necessary in order to predict small LOCAs as accurately as large LOCAs

  12. RELAP5/MOD2 analysis of LOFT Experiment L9-3

    International Nuclear Information System (INIS)

    Birchley, J.C.

    1992-04-01

    An analysis has been performed of LOFT Experiment L9-3, a loss-of-feedwater anticipated transient without trip, in order to support the validation of RELAP5/MOD2. Experiment L9-3 exhibited a rapid boildown of the steam generator, following the loss of feed, with the reactor remaining close to its initial power until the steam generator tubes became sufficiently uncovered for primary to secondary heat transfer to be significantly reduced. The ensuing heat up of the primary fluid resulted in a reduction in power induced by the moderator feedback. The primary system pressure increased to the safety relief valve setpoint, before the fall in reactor power allowed the mismatch between primary system heat input and heat removal via the steam generator to be accommodated by cycling of the pilot operated relief valve (PORV). Comparison between calculation and data shows generally good agreement, though with discrepancies in some areas. Weaknesses in the code's treatment of interphase drag and in the representation of the pressuriser spray are indicated, although a shortage of definitive data, particularly in the steam generator, may also be a factor. The overprediction of interphase drag led to a tendency to underpredict the initial inventory in the steam generator and also, perhaps, to overpredict the steam generator heat transfer while the tubes were being uncovered. There is indication that the pressuriser vapour region conditions were close to equilibrium during spray operation. The point kinetics model in RELAP5/MOD2 proved a viable means of representing the power history for this transient

  13. RELAP5/MOD2 code assessment using a LOFT L2-3 loss of coolant experiment

    International Nuclear Information System (INIS)

    Bang, Young Seok; Chung, Bub Dong; Kim, Hho Jung

    1990-01-01

    The LOFT LOCE L2-3 was simulated using the RELAP5/MOD2 Cycle 36.04 code to assess its capability in predicting the thermal-hydraulic phenomena in LBLOCA of the PWR. The reactor vessel was simulated with two core channels and split downcomer modeling for a base case calculation using the frozen code. The result of the base calculation showed that the code predicted the hydraulic behavior, and the blowdown thermal response at high power region of the core in a reasonable range and that the code had deficiencies in the critical flow model during subcooled-two-phase transition period, in the CHF correlation at high mass flux and in the blowdown rewet criteria. An overprediction of coolant inventory due to the deficiencies yielded the poor prediction of reflood thermal response. A Sensitivity calculation with an updated version from RELAP5/MOD2 Cycle 36.04 improved the prediction of the rewet phenomena

  14. Application of RELAP5/MOD3.1 code to the LOFT test L3-6

    International Nuclear Information System (INIS)

    Pylev, S.S.; Roginskaja, V.L.

    1998-02-01

    A calculation of LOFT Experiment L3-6, a small break equivalent to a 4-in diameter rupture in the cold leg of a four-loop commercial pressurized water reactor, has been performed to help validate RELAP5/MOD3.1 for this application. The version of the code to be used is SCDAP/RELAP5/MOD3.1.8d0. Three calculations were carried out in order to study the sensitivity to change break nozzle superheated discharge coefficient. Conducted comparative analysis of the LOFT L3-6 experiment shows on the whole a reasonable agreement between calculated data. Some discrepancies in the system pressure do not distort a picture of the transient. 6 refs

  15. Application of RELAP5/MOD3.1 code to the LOFT test L3-6

    Energy Technology Data Exchange (ETDEWEB)

    Pylev, S.S.; Roginskaja, V.L.

    1998-02-01

    A calculation of LOFT Experiment L3-6, a small break equivalent to a 4-in diameter rupture in the cold leg of a four-loop commercial pressurized water reactor, has been performed to help validate RELAP5/MOD3.1 for this application. The version of the code to be used is SCDAP/RELAP5/MOD3.1.8d0. Three calculations were carried out in order to study the sensitivity to change break nozzle superheated discharge coefficient. Conducted comparative analysis of the LOFT L3-6 experiment shows on the whole a reasonable agreement between calculated data. Some discrepancies in the system pressure do not distort a picture of the transient. 6 refs.

  16. LOFT/L3-, Loss of Fluid Test, 7. NRC L3 Small Break LOCA Experiment

    International Nuclear Information System (INIS)

    1992-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: This was the seventh in the NRC L3 Series of small-break LOCA experiments. A 2.5-cm (10-in.) cold-leg non-communicative-break LOCA was simulated. The experiment was conducted on 20 June 1980

  17. LOFT/L3-6, Loss of Fluid Test, 6. NRC L3 Small Break LOCA Experiment

    International Nuclear Information System (INIS)

    1992-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: This was the sixth in the NRC L3 Series of small-break LOCA experiments. A 10-cm (2.5-in.) cold-leg non-communicative-break LOCA was simulated. Pumps were running. The experiment was conducted on 10 December 1980

  18. RELAP5/MOD2 post-test calculation of the OECD LOFT experiment LP-SB-2

    International Nuclear Information System (INIS)

    Perez, J.; Mendizabal, R.

    1992-04-01

    This document presents the analysis of the OECD LOFT LP-SB-2 Experiment performed by the Consejo de Seguridad Nuclear of Spain working group making use of RELAP5/MOD2 in the frame of the Spanish LOFT Project. LB-SB-2 experiment studies the effect of a delayed pump trip in a small break LOCA scenario with a 3-inch equivalent diameter break in the hot leg of a commercial PWR

  19. Assessment of predictive capability of REFLA/TRAC code for large break LOCA transient in PWR using LOFT L2-5 test data

    International Nuclear Information System (INIS)

    Akimoto, Hajime; Ohnuki, Akira; Murao, Yoshio

    1994-03-01

    The REFLA/TRAC code is a best estimate code developed at Japan Atomic Energy Research Institute (JAERI) to provide advanced predictions of thermal hydraulic transient in light water reactors (LWRs). The REFLA/TRAC code uses the TRAC-PF1/MOD1 code as the framework of the code. The REFLA/TRAC code is expected to be used for the calibration of licensing codes, accident analysis, accident simulation of LWRs, and design of advanced LWRs. Several models have been implemented to the TRAC-PF1/MOD1 code at JAERI including reflood model, condensation model, interfacial and wall friction models, etc. These models have been verified using data from various separate effect tests. This report describes an assessment result of the REFLA/TRAC code, which was performed to assess the predictive capability for integral system behavior under large break loss of coolant accident (LBLOCA) using data from the LOFT L2-5 test. The assessment calculation confirmed that the REFLA/TRAC code can predict break mass flow rate, emergency core cooling water bypass and clad temperature excellently in the LOFT L2-5 test. The CPU time of the REFLA/TRAC code was about 1/3 of the TRAC-PF1/MOD1 code. The REFLA/TRAC code can perform stable and fast simulation of thermal hydraulic behavior in PWR LBLOCA with enough accuracy for practical use. (author)

  20. Blowdown hydraulic influence on core thermal response in LOFT nuclear experiment L2-3

    International Nuclear Information System (INIS)

    Reeder, D.L.

    1979-01-01

    Experimental research into pressurized water reactor (PWR) loss-of-coolant phenomena conducted in the Loss-of-Fluid Test (LOFT) facility has given results indicating that for very large pipe breaks the core thermal response is tightly coupled to the fluid hydraulic phenomena during the blowdown phase of the loss-of-coolant transient. This summary presents and discusses data supporting this conclusion. LOFT Loss-of-Coolant Experiment (LOCE) L2-3 simulated a complete double-ended offset shear break of a primary coolant reactor vessel inlet pipe in a commercial PWR. The LOFT system conditions at experiment initiation were: fuel rod maximum linear heat generation rate (MLHGR) of 39.4 +- 3 kW/m, hot leg temperature of 593 +- 3 K, core ΔT of 32.2 +- 4 K, system pressure of 15.06 +- 0.03 MPa, and flow rate/system volume of 25.6 +- 0.8 kg/m 3 . These conditions are typical of those in commercial PWR systems at normal operating conditions

  1. Stress analysis of the LOFT modular DTT flowmeter for LOCE transients (L1-5 and L2-4)

    International Nuclear Information System (INIS)

    Mosby, W.R.

    1978-01-01

    An analysis is presented of combined stresses in the LOFT Modular DTT for specified temperature gradients. All combined stress intensities are shown to stay within applicable allowable stress intensities. A fatigue analysis is also presented which indicates that the LOFT Modular DTT will withstand 70,000 blowdown cycles. The LOFT Modular DTT is shown to meet the Class 1 stress requirments. A stress analysis of the tab region of the newly designed MDTT tab-type shroud is included. This stress analysis shows that the Class 1 stress requirements are met by the tab-type MDTT shroud design and that this design imposes no fatigue life limitation on the MDTT

  2. Experiment data report for LOFT nonnuclear Test L1-4

    International Nuclear Information System (INIS)

    Batt, D.L.

    1977-07-01

    Test L1-4 was the fourth in a series of five nonnuclear isothermal blowdown tests conducted by the Loss of Fluid Test (LOFT) Program. Test L1-4 was the first Nuclear Regulatory Commission standard problem (International Problem No. 5 and U.S. Problem No. 7) experiment conducted at LOFT. Data from this test will be compared with predictions generated by the standard problem participants. For this test the LOFT Facility was configured to simulate a loss-of-coolant accident in a large pressurized water reactor resulting from a 200% double-ended offset shear break in a cold leg of the primary coolant system. A hydraulic core simulator assembly was installed in place of the nuclear core. The initial conditions in the primary coolant system intact loop were temperature at 279 0 C, gauge pressure at 15.65 MPa, and intact loop flow at 268.4 kg/s. During system depressurization into a simulated containment, emergency core cooling water was injected into the primary coolant system cold leg to provide data on the effects of emergency core cooling on system thermalhydraulic response

  3. Best estimate analysis of LOFT L2-5 with CATHARE: uncertainty and sensitivity analysis

    Energy Technology Data Exchange (ETDEWEB)

    JOUCLA, Jerome; PROBST, Pierre [Institute for Radiological Protection and Nuclear Safety, Fontenay-aux-Roses (France); FOUET, Fabrice [APTUS, Versailles (France)

    2008-07-01

    The revision of the 10 CFR50.46 in 1988 has made possible the use of best-estimate codes. They may be used in safety demonstration and licensing, provided that uncertainties are added to the relevant output parameters before comparing them with the acceptance criteria. In the safety analysis of the large break loss of coolant accident, it was agreed that the 95. percentile estimated with a high degree of confidence should be lower than the acceptance criteria. It appeared necessary to IRSN, technical support of the French Safety Authority, to get more insight into these strategies which are being developed not only in thermal-hydraulics but in other fields such as in neutronics. To estimate the 95. percentile with a high confidence level, we propose to use rank statistics or bootstrap. Toward the objective of assessing uncertainty, it is useful to determine and to classify the main input parameters. We suggest approximating the code by a surrogate model, the Kriging model, which will be used to make a sensitivity analysis with the SOBOL methodology. This paper presents the application of two new methodologies of how to make the uncertainty and sensitivity analysis on the maximum peak cladding temperature of the LOFT L2-5 test with the CATHARE code. (authors)

  4. Prediction of LOFT L1-4 experiment

    International Nuclear Information System (INIS)

    Soda, Kunihisa; Sasaki, Shinobu; Akimoto, Masayuki; Koizumi, Yasuo; Araya, Fumimasa

    1977-10-01

    LOFT L1-4 experimental results were predicted by LOFT Analysis Group and Code Development Group using RELAP-4J and ALARM-P1 respectively. The input data prepared by the former group were used in both the analyses. Thus any differences in the results should stem from the differences in code performance characteristics of the two codes. (1) The coolant behaviors predicted by RELAP-4J and ALARM-P1 are in good agreement although some differences do exist between these two calculation models. (2) Large difference is seen in coolant flow rate across the pump. The coast down and the flow rate by ALARM-P1 are larger and smaller respectively than by RELAP-4J. (3) An explicit method of the ALARM-P1 leads to unstable calculation at a T shaped junction when one of the two volumes connected by the junction is filled with subcooled water. (4) Coolant flow in the downcomer, heat transfer to and from the steam generator secondary and suppression tank behavior must be modified to better predict the experimental results. (5) Additional instrumentation in reflood assist and ECC injection lines are necessary to better nderstand the coolant behavior. (auth.)

  5. Experiment prediction for Loft Nonnuclear Experiment L1-4

    International Nuclear Information System (INIS)

    White, J.R.; Berta, V.T.; Holmstrom, H.L.O.

    1977-04-01

    A computer analysis, using the WHAM and RELAP4 computer codes, was performed to predict the LOFT system thermal-hydraulic response for Experiment L1-4 of the nonnuclear (isothermal) test series. Experiment L1-4 will simulate a 200 percent double-ended offset shear in the cold leg of a four-loop large pressurized water reactor. A core simulator will be used to provide a reactor vessel pressure drop representative of the LOFT nuclear core. Experiment L1-4 will be initiated with a nominal isothermal primary coolant temperature of 282.2 0 C, a pressurizer pressure of 15.51 MPa, and a primary coolant flow of 270.9 kg/s. In general, the predictions of saturated blowdown for Experiment Ll-4 are consistent with the expected system behavior, and predicted trends agree with results from Semiscale Test S-01-4A, which simulated the Ll-4 experiment conditions

  6. PBF/LOFT Lead Rod Test Program experiment predictions document

    International Nuclear Information System (INIS)

    Varacalle, D.J.; Cox, W.R.; Niebruegge, D.A.; Seiber, S.J.; Brake, T.E.; Driskell, W.E.; Nigg, D.W.; Tolman, E.L.

    1978-12-01

    The PBF/LOFT Lead Rod (LLR) Test Program is being conducted to provide experimental information on the behavior of nuclear fuel under normal and accident conditions in the Power Burst Facility (PBF) at the Idaho National Engineering Laboratory. The PBF/LLR tests are designed to simulate the test conditions for the LOFT Power Ascension Tests L2-3 through L2-5. The test program has been designed to provide a parametric evaluation of the LOFT fuel (center and peripheral modules) over a wide range of power. This report presents the experiment predictions for the three four-rod LOCA tests

  7. LOFT/L9-3, Loss of Fluid Test, Anticipated Transients with Multiple Failures

    International Nuclear Information System (INIS)

    1992-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: This was the third of the NRC L9 series of experiments on Anticipated Transients with Multiple Failures. Loss-of-feedwater effects were studied. The experiment was conducted on 7 April 1982

  8. LOFT/L6-7, Loss of Fluid Test, Anticipated Transients with Multiple Failures

    International Nuclear Information System (INIS)

    1992-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: This was the seventh in the NRC L6 Series of Anticipated Transients experiments. Rapid secondary side induced cooldown was studied. The experiment was conducted on 31 September 1981

  9. BEMUSE Phase III Report - Uncertainty and Sensitivity Analysis of the LOFT L2-5 Test

    International Nuclear Information System (INIS)

    Bazin, P.; Crecy, A. de; Glaeser, H.; Skorek, T.; Joucla, J.; Probst, P.; Chung, B.; Oh, D.Y.; Kyncl, M.; Pernica, R.; Macek, J.; Meca, R.; Macian, R.; D'Auria, F.; Petruzzi, A.; Perez, M.; Reventos, F.; Fujioka, K.

    2007-02-01

    This report summarises the various contributions (ten participants) for phase 3 of BEMUSE: Uncertainty and Sensitivity Analyses of the LOFT L2-5 experiment, a Large-Break Loss-of-Coolant-Accident (LB-LOCA). For this phase, precise requirements step by step were provided to the participants. Four main parts are defined, which are: 1. List and uncertainties of the input uncertain parameters. 2. Uncertainty analysis results. 3. Sensitivity analysis results. 4. Improved methods, assessment of the methods (optional). 5% and 95% percentiles have to be estimated for 6 output parameters, which are of two kinds: 1. Scalar output parameters (First Peak Cladding Temperature (PCT), Second Peak Cladding Temperature, Time of accumulator injection, Time of complete quenching); 2. Time trends output parameters (Maximum cladding temperature, Upper plenum pressure). The main lessons learnt from phase 3 of the BEMUSE programme are the following: - for uncertainty analysis, all the participants use a probabilistic method associated with the use of Wilks' formula, except for UNIPI with its CIAU method (Code with the Capability of Internal Assessment of Uncertainty). Use of both methods has been successfully mastered. - Compared with the experiment, the results of uncertainty analysis are good on the whole. For example, for the cladding temperature-type output parameters (1. PCT, 2. PCT, time of complete quenching, maximum cladding temperature), 8 participants out of 10 find upper and lower bounds which envelop the experimental data. - Sensitivity analysis has been successfully performed by all the participants using the probabilistic method. All the used influence measures include the range of variation of the input parameters. Synthesis tables of the most influential phenomena and parameters have been plotted and participants will be able to use them for the continuation of the BEMUSE programme

  10. Contribution of the mathematical modelling of knowledge to the evaluation of uncertainty margins of a LBLOCA transient (LOFT-L2-5)

    International Nuclear Information System (INIS)

    Baccou, J.; Chojnacki, E.

    2007-01-01

    This work is devoted to some recent developments in uncertainty analysis of the computer code responses used for accident management procedures in nuclear industry. The classical probabilistic approach to evaluate uncertainties is recalled. In this case, the statistical treatment of the code responses is based on the use of order statistics. It provides direct estimations of relevant statistical measures for safety studies. However, the lack of knowledge about uncertainty sources can deteriorate the decision-making. To respect the real state of knowledge, a second model, based on the Dempster-Shafer theory is introduced. It allows to mix the probabilistic approach with the possibility theory that is more appropriate when few information is available. An application of both methodologies to the uncertainty analysis of a LBLOCA transient (LOFT-L2-5) is given

  11. Uncertainty and sensitivity analysis of the LOFT L2-5 test: Results of the BEMUSE programme

    International Nuclear Information System (INIS)

    Crecy, A. de; Bazin, P.; Glaeser, H.; Skorek, T.; Joucla, J.; Probst, P.; Fujioka, K.; Chung, B.D.; Oh, D.Y.; Kyncl, M.; Pernica, R.; Macek, J.; Meca, R.; Macian, R.; D'Auria, F.; Petruzzi, A.; Batet, L.; Perez, M.; Reventos, F.

    2008-01-01

    This paper presents the results and the main lessons learnt from the phase 3 of BEMUSE, an international benchmark activity sponsored by the Committee on the Safety of Nuclear Installations [CSNI: Committee on the Safety of Nuclear Installations (NEA, OECD), 2007. BEMUSE Phase III Report. NEA/CSNI R(2007) 4, October 2007] of the OECD/NEA. The phase 3 of BEMUSE aimed at performing Uncertainty and Sensitivity Analyses of thermal-hydraulic codes used for the calculation of LOFT L2-5 experiment, which simulated a Large-Break Loss-of-Coolant-Accident (LB-LOCA). Eleven participants coming from ten organisations and eight countries took part in this benchmark. In the first section of this paper, the context of BEMUSE is described as well as the methods used by the participants. In the second section, the results of the benchmark are presented. The majority of the participants find uncertainty bands which envelop the experimental data fairly well, however the width of these bands is much diverged. A synthesis of the sensitivity analysis results has been made and is expected to provide a useful basis for further uncertainty analysis dealing with LB-LOCA. Finally, recommendations are given both for uncertainty and sensitivity analysis

  12. Revisiting LOFT L2-5 large break test in BEMUSE project context. Sensitivity studies

    International Nuclear Information System (INIS)

    Perez, Marina; Batet, Lluis; Pretel, Carme; Reventos, Francesc

    2005-01-01

    Full text of publication follows: Best estimate codes simulate NPPs behavior in principle without any special conservative assumptions. Due to several factors like code solution methods or user effects, the output parameters calculated have an uncertainty associated. The quantification of the these uncertainties becomes crucial when a safety statement is to be made. It is in this scope that GAMA group from CSNI (OECD/NEA) proposed the international BEMUSE project (Best Estimate - Uncertainty and Sensitivity Evaluation) having as main objective the evaluation of different methodologies for the uncertainty and sensitivity analysis of best-estimate code calculations. A number of methodologies prepared in different countries are used in the development of the project activities. The program work consists of 6 phases and currently the first two have already been concluded. Phase II consists in revisiting the ISP-13, the LOFT loss of coolant experiment L2-5 which simulated a double ended 200% cold leg break of a commercial PWR simultaneous with a loss of site power. In order to connect phase II with phase III, in which the uncertainty analysis will be carried out, quite a large number of sensitivity analysis have been performed by simulating system failures and varying fuel elements parameters among others. The presentation will focus on the results of the sensitivity analysis as well as its importance with regards to the uncertainty studies. The methodology used by UPC team was developed by ENUSA and the work is supported by the Spanish regulatory organization. (authors)

  13. PBF/LOFT Lead Rod Test Program experiment operating specification

    International Nuclear Information System (INIS)

    Varacalle, D.J. Jr.

    1978-11-01

    The PBF/LOFT Lead Rod (LLR) Test Program is being conducted to provide experimental information on the behavior of nuclear fuel under normal and accident conditions in the Power Burst Facility at the Idaho National Engineering Laboratory. Understanding the behavior of light-water reactors (LWR) under loss-of-coolant conditions is a major objective of the NRC Reactor Safety Research Program. The Loss of Fluid Test (LOFT) facility is the major testing facility to evaluate the systems response of an LWR over a wide range of Loss of Coolant Experment (LOCE) conditions. As such, the LOFT core is intended to be used for sequential LOCE tests provided no significant fuel rod failures occur. The PFB/LLR tests are designed to simulate the test conditions for the LOFT Power Ascension Tests L2-2 through L2-5. The test program has been designed to provide a parametric evaluation of the LOFT fuel over a wide range of power. Thus, a relatively accurate assessment of the state of the LOFT core after the completion of each subtest and the anticipated effect of the next test can be obtained by utilizing a combination of LLR test data and analytical predictions. Specifications for the test program are presented

  14. Through analysis of LOFT L2-3 by THYDE-P code

    International Nuclear Information System (INIS)

    Hirano, Masashi

    1981-10-01

    A through calculation of Experiment L2-3 of the Loss-of-Fluid Test (LOFT) Facility Power Ascension Series (Experiment Series L2) was performed with the THYDE-P code. The specific objectives of Experiment L2-3 were to determine the thermal-hydraulic behavior of the nuclear core and the thermal-mechanical response of the fuel rod cladding with a maximum linear heat generation rate of 39.4 kW/m. The THYDE-P code is a computer code to analyze both the blowdown and refill-reflood phases of loss-of-coolant accidents (LOCAs) of pressurized water reactors (PWRs) without a change in the methods and the models and is now under verification study and modification. The present calculation was performed by best estimate (BE) options as Sample Calculation Run 40, which is a portion of a series of THYDE-P sample calculations. The calculation was carried out from test initiation until complete submersion of the core volume with subcooled water, i.e. about 60 sec. The trend of the calculated cladding surface temperature was in good agreement with that of the experimental results. (author)

  15. LOFT drag-disc turbine tansducer shroud and cover weld analysis and evaluation

    International Nuclear Information System (INIS)

    Martinell, J.S.

    1978-01-01

    The results are presented of stress analysis and evaluation of the drag-disc turbine transducer (DTT) shroud and cover welds used in the Loss-of-Fluid Test (LOFT) facility. The analysis concerns LOFT DTTs currently installed and/or planned for use in future experiments by LOFT Experimental Measurements Branch personnel. Thermal/hydraulic conditions for Experiments L1-5 and L2-4 (for all nuclear tests) were used to predict the loads experienced by the various shroud and cover welds. The results of this analysis include minimum fatigue life for all subject welds, and Applied Mechanics Branch recommendations for improving the structural integrity of critically stressed welds

  16. Benchmarking of LOFT LRTS-COBRA-FRAP safety analysis model

    International Nuclear Information System (INIS)

    Hanson, G.H.; Atkinson, S.A.; Wadkins, R.P.

    1982-05-01

    The purpose of this work was to check out the LOFT LRTS/COBRA-IV/FRAP-T5 safety-analysis models against test data obtained during a LOFT operational transient in which there was a power and fuel-temperature rise. LOFT Experiment L6-3 was an excessive-load-increase anticipated transient test in which the main steam-flow-control valve was driven from its operational position to full-open in seven seconds. The resulting cooldown and reactivity-increase transients provide a good benchmark for the reactivity-and-power-prediction capability of the LRTS calculations, and for the fuel-bundle and fuel-rod temperature-response analysis capability of the LOFT COBRA-IV and FRAP-T5 models

  17. Experiment data report for LOFT anticipated transient-without-scram Experiment L9-3

    International Nuclear Information System (INIS)

    Bayless, P.D.; Divine, J.M.

    1982-05-01

    Selected pertinent and uninterpreted data from the third anticipated transient with multiple failures experiment (Experiment L9-3) conducted in the Loss-of-Fluid Test (LOFT) facility are presented. The LOFT facility is a 50-MW(t) pressurized water reactor (PWR) system with instruments that measure and provide data on the system thermal-hydraulic and nuclear conditions. The operation of the LOFT system is typical of large [approx. 1000 MW(e)], commercial PWR operations. Experiment L9-3 simulated a loss-of-feedwater anticipated transient without scram. The loss-of-feedwater accident led to an increase in the primary coolant system temperature and pressure. Both the experiment power-operated relief valve (PORV) and safety relief valve opened and were able to limit and control the pressure transient. The plant was then recovered with the control rods still withdrawn by injecting 7200-ppM borated water, manually cycling the PORV and feeding and bleeding the steam generator

  18. Experiment data report for LOFT anticipated transient without scram Experiment L9-4

    International Nuclear Information System (INIS)

    Batt, D.L.; Divine, J.M.; McKenna, K.J.

    1982-11-01

    Selected pertinent and uninterpreted data from the fourth anticipated transient with multiple failures experiment (Experiment L9-4) conducted on September 24, 1982, in the Loss-of-Fluid Test (LOFT) facility are presented. The LOFT facility is a 50-MW(t) pressurized water reactor (PWR) system with instruments that measure and provide data on the system's thermal-hydraulic and nuclear conditions. The operation of the LOFT system is typical of large [approx. 1000 MW(e)], commercial PWR operations. Experiment L9-4 simulated a loss-of-offsite-power anticipated transient without reactor scram. The loss-of-offsite-power accident led to an increase in the primary coolant system temperature and pressure. The experiment safety relief valve opened and was able to limit and control the pressure transient. In addition, subsequent heat generation was dissipated by the auxiliary feedwater flow in the secondary coolant system until the reactor was scrammed at experiment termination

  19. RELAP5/MOD2 calculation of OECD LOFT test LP-FW-01

    International Nuclear Information System (INIS)

    Croxfod, M.G.; Harwood, C.; Hall, P.C.

    1992-04-01

    RELAP5/MOD2 is being used by GDCD for calculation of certain small break loss-of-coolant accidents and pressurized transients in the Sizewell ''B'' PWR. To test the ability of RELAP5/MOD2 to model the primary feed-and-bleed recovery procedure following a complete loss- of-feedwater event, post test calculations have been carried out of OECD LOFT test LP-FW-01. This report describes the comparison between the code calculations and the test data. It is found that although the standard version of RELAP5/MOD2 gives a reasonable prediction of the experimental transient, the long term pressure history is better calculated with a modified code version containing a revised horizontal stratification entrainment model. The latter allows an improved calculation of entrainment of liquid from the hot leg into the surge line. RELAP5/MOD2 is found to give a more accurate simulation of the experimental transient than was achieved in previous UK studies using RETRAN-02/MOD2

  20. The use of Monte-Carlo simulation and order statistics for uncertainty analysis of a LBLOCA transient (LOFT-L2-5)

    International Nuclear Information System (INIS)

    Chojnacki, E.; Benoit, J.P.

    2007-01-01

    Best estimate computer codes are increasingly used in nuclear industry for the accident management procedures and have been planned to be used for the licensing procedures. Contrary to conservative codes which are supposed to give penalizing results, best estimate codes attempt to calculate accidental transients in a realistic way. It becomes therefore of prime importance, in particular for technical organization as IRSN in charge of safety assessment, to know the uncertainty on the results of such codes. Thus, CSNI has sponsored few years ago (published in 1998) the Uncertainty Methods Study (UMS) program on uncertainty methodologies used for a SBLOCA transient (LSTF-CL-18) and is now supporting the BEMUSE program for a LBLOCA transient (LOFT-L2-5). The large majority of BEMUSE participants (9 out of 10) use uncertainty methodologies based on a probabilistic modelling and all of them use Monte-Carlo simulations to propagate the uncertainties through their computer codes. Also, all of 'probabilistic participants' intend to use order statistics to determine the sampling size of the Monte-Carlo simulation and to derive the uncertainty ranges associated to their computer calculations. The first aim of this paper is to remind the advantages and also the assumptions of the probabilistic modelling and more specifically of order statistics (as Wilks' formula) in uncertainty methodologies. Indeed Monte-Carlo methods provide flexible and extremely powerful techniques for solving many of the uncertainty propagation problems encountered in nuclear safety analysis. However it is important to keep in mind that probabilistic methods are data intensive. That means, probabilistic methods cannot produce robust results unless a considerable body of information has been collected. A main interest of the use of order statistics results is to allow to take into account an unlimited number of uncertain parameters and, from a restricted number of code calculations to provide statistical

  1. Experiment data report for LOFT nonnuclear test L1-3

    International Nuclear Information System (INIS)

    Millar, G.M.

    1977-04-01

    Test L1-3 was the third in a series of five nonnuclear isothermal blowdown tests conducted by the Loss of Fluid Test (LOFT) Program. For this test the LOFT Facility was configured to simulate a loss-of-coolant accident in a large pressurized water reactor resulting from a 200 percent double-ended shear break in a cold leg of the primary coolant system. A hydraulic core simulator assembly was installed in place of the nuclear core. The initial conditions in the primary coolant system intact loop were: temperature at 540 0 F, pressure at 2256 psig, and loop flow at 2.34 x 10 6 lbm/hr. During system depressurization, emergency core cooling water was specified to be injected into the lower plenum of the reactor vessel using an accumulator, a low-pressure injection system pump, and a high-pressure injection system pump to provide data on the effects of emergency core cooling on the system thermal-hydraulic response. Injection into the lower plenum was initiated from the high- and low-pressure injection systems. Injection from the accumulator, however, was not initiated because a valve was inadvertently left closed. The experiment, therefore, was not completely successful in that one of the objectives outlined in the experiment operating specification for this test was not accomplished. Test L1-3 was repeated at Test L1-3A to meet the experimental requirements. Despite these difficulties, Test L1-3 did provide very valuable data to verify experiment repeatability

  2. Experiment data report for LOFT Boron dilution Experiment L6-6

    International Nuclear Information System (INIS)

    Stitt, B.D.; Divine, J.M.

    1982-06-01

    Selected pertinent and uninterpreted data from the sixth anticipated transient experiment (Experiment L6-6) conducted in the Loss-of-Fluid Test (LOFT) facility are presented. Experiment L6-6 simulated a boron dilution accident by injecting demineralized water into the primary coolant system (PCS) at a rate of 0.47 L/s while the reactor was in a cold shutdown condition with the control rods withdrawn. System pressure was maintained at approximately 285 kPa throughout the experiment. The experiment was divided into two parts. In the first part, L6-6A, a recirculation flow of 4.7 L/s was maintained through the PCS and criticality was achieved 7416 +- 10 s after the initiation of the dilution flow. The second part, L6-6B, was identical to L6-6A except that a recirculation flow of 9.5 L/s was maintained and criticality occurred at 8058 +- 10 s

  3. Experiment predictions of LOFT reflood behavior using the RELAP4/MOD6 code

    International Nuclear Information System (INIS)

    Lin, J.C.; Kee, E.J.; Grush, W.H.; White, J.R.

    1978-01-01

    The RELAP4/MOD6 computer code was used to predict the thermal-hydraulic transient for Loss-of-Fluid Test (LOFT) Loss-of-Coolant Accident (LOCA) experiments L2-2, L2-3, and L2-4. This analysis will aid in the development and assessment of analytical models used to analyze the LOCA performance of commercial power reactors. Prior to performing experiments in the LOFT facility, the experiments are modeled in counterpart tests performed in the nonnuclear Semiscale MOD 1 facility. A comparison of the analytical results with Semiscale data will verify the analytical capability of the RELAP4 code to predict the thermal-hydraulic behavior of the Semiscale LOFT counterpart tests. The analytical model and the results of analyses for the reflood portion of the LOFT LOCA experiments are described. These results are compared with the data from Semiscale

  4. Summary of important results and SCDAP/RELAP5 analysis for OECD LOFT experiment LP-FP-2

    International Nuclear Information System (INIS)

    Coryell, E.W.

    1994-04-01

    This report summarizes significant technical findings from the LP-FP-2 Experiment sponsored by OECD. It was the second, and final, fission product experiment conducted in the Loss-of-Fluid Test (LOFT) facility at the Idaho National Engineering Laboratory. The overall technical objective of the test was to contribute to the understanding of fuel rod behavior, hydrogen generation, and fission product release, transport, and deposition during a V-sequence accident scenario that resulted in severe core damage. An 11 by 11 test bundle, comprised of 100 pre-pressurized fuel rods, 11 control rods, and 10 instrumented guide tubes, was surrounded by an insulating shroud and contained in a specially designed central fuel module, that was inserted into the LOFT reactor. The simulated transient was a V-sequence loss-of-coolant accident scenario featuring a pipe break in the low pressure injection system line attached to the hot leg of the LOFT broken loop piping. The transient was terminated by reflood of the reactor vessel when the outer wall shroud temperature reached 1517 K. With sustained fission power and heat from oxidation and metal-water reactions, elevated temperatures resulted in zircaloy melting, fuel liquefaction, material relocation, and the release of hydrogen, aerosols, and fission products. A description and evaluation of the major phenomena, based upon the response of on line instrumentation, analysis of fission product data, post-irradiation examination of the fuel bundle, and calculations using the SCDAP/RELAP5 computer code, are presented

  5. Summary of important results and SCDAP/RELAP5 analysis for OECD LOFT experiment LP-FP-2

    Energy Technology Data Exchange (ETDEWEB)

    Coryell, E.W. [EG and G Idaho, Inc., Idaho Falls, ID (United States)

    1994-04-01

    This report summarizes significant technical findings from the LP-FP-2 Experiment sponsored by the Organization of Economic Cooperation and Development (OECD). It was the second, and final, fission product experiment conducted in the Loss-of-Fluid Test (LOFT) facility at the Idaho National Engineering Laboratory. The overall technical objective of the test was to contribute to the understanding of fuel rod behavior, hydrogen generation, and fission product release, transport, and deposition during a V-sequence accident scenario that resulted in severe core damage. An 11 by 11 test bundle, comprised of 100 prepressurized fuel rods, 11 control rods, and 10 instrumented guide tubes, was surrounded by an insulating shroud and contained in a specially designed central fuel module, that was inserted into the LOFT reactor. The simulated transient was a V-sequence loss-of-coolant accident scenario featuring a pipe break in the low pressure injection system line attached to the hot leg of the LOFT broken loop piping. The transient was terminated by reflood of the reactor vessel when the outer wall shroud temperature reached 1517 K. With sustained fission power and heat from oxidation and metal-water reactions, elevated temperatures resulted in zircaloy melting, fuel liquefaction, material relocation, and the release of hydrogen, aerosols, and fission products. A description and evaluation of the major phenomena, based upon the response of on line instrumentation, analysis of fission product data, postirradiation examination of the fuel bundle, and calculations using the SCDAP/RELAP5 computer code, are presented.

  6. Analysis of LOFT (L1-2) experiment by code RELAP-4J

    International Nuclear Information System (INIS)

    Tasaka, Kanji; Soda, Kunihisa; Shiba, Masayoshi; Kaminaga, Humito

    1977-04-01

    An analysis of the results in LOFT L1-2 LOCE (Loss of Coolant Experiment) was made by the computer code RELAP-4J. The L1-2 experiment is a simple isothermal blowdown test with a core simulator and no ECC activation. It provides the basis for future LOCE with a nuclear core and ECC activation. The results of the analysis lead to the following conclusions. (1) The calculated system pressure transient agrees well with experiment. Primary controlling factors for the calculation are (a) flow resistances of the steam generator simulator, pump simulator and discharge nozzle in the broken loop, (b) mixture level in the downcomer and inlet volume of the operating loop steam generator, and (c) stored heat of the downcomer structure. (2) The pressurizer pressure decreases rapidly, compared with experiment, possibly because the flow resistance in the surge line is smaller than the actual one. Further experiment and analysis are necessary in this respect. (3) The calculated density transient in the cold leg agrees well with experiment. Agreement is not good in the hot leg, however. The discrepancy is possibly caused by the non-homogeneous flow of coolant in the hot leg due to low flow rate. (4) Effect of the pump characteristics on analytical result is insignificant in the isothermal test. However, in the future nuclear test, the effect will be significant because of large steam generation in the core, so measurement of the pump characteristics and improvement of the pump model are necessary. (auth.)

  7. Simulation of LOFT anticipated-transient experiments L6-1, L6-2, and L6-3 using TRAC-PF1/MOD1

    International Nuclear Information System (INIS)

    Sahota, M.S.

    1984-01-01

    Anticipated-transient experiments L6-1, L6-2, and L6-3, performed at the Loss-of-fluid Test (LOFT) facility, are analyzed using the latest released version of the Transient Reactor Analysis Code (TRAC-PF1/MOD1). The results are used to assess TRAC-PF1/MOD1 trip and control capabilities, and predictions of thermal-hydraulic phenomena during slow transients. Test L6-1 simulated a loss-of-stream load in a large pressurized-water reactor (PWR), and was initiated by closing the main steam-flow control valve (MSFCV) at its maximum rate, which reduced the heat removal from the secondary-coolant system and increased the primary-coolant system pressure that initiated a reactor scram. Test L6-2 simulated a loss-of-primary coolant flow in a large PWR, and was initiated by tripping the power to the primary-coolant pumps (PCPs) allowing the pumps to coast down. The reduced primary-coolant flow caused a reactor scram. Test L6-3 simulated an excessive-load increase incident in a large PWR, and was initiated by opening the MSFCV at its maximum rate, which increased the heat removal from the secondary-coolant system and decreased the primary-coolant system pressure that initiated a reactor scram. The TRAC calculations accurately predict most test events. The test data and the calculated results for most parameters of interest also agree well

  8. ICAP [International Code Assessment and Applications Program] assessment of RELAP5/MOD2, Cycle 36.05 against LOFT [Loss of Fluid Test] Small Break Experiment L3-7

    International Nuclear Information System (INIS)

    Lee, Euy-Joon; Chung, Bud-Dong; Kim, Hho-Jung

    1990-04-01

    The LOFT small break (1 in-dia) experiment L3-7 has been analyzed using the reactor thermal hydraulic analysis code RELAP5/MOD2, Cycle 36.05. The base calculation (Case A) was completed and compared with the experimental data. Three types of sensitivity studies (Cases B, Cm, and D) were carried out to investigate the effects of (1) break discharge coefficient Cd, (2) pump two-phase difference multiplier and (3) High Pressure Injection System (HPIS) capacity on major thermal and hydraulic (T/H) parameters. A nodalization study (Case E) was conducted to assess the phenomena with a simplified nodalization. The results indicate that Cd of 0.9 and 0.1 fit to the single discharge flow rate of Test L3-7 best among the tried cases. The pump two-phase multiplier has little effects on the T/H parameters because of the low discharge flow rate and the early pump coast down in this smaller size SBLOCA. But HPIS capacity has a very strong influence on parameters such as pressure, flow and temperature. It is also shown that a simplified nodalization could accomodate the dominant T/H phenomena with the same degree of code accuracy and efficiency

  9. LOFT/LP-FP-2, Loss of Fluid Test, Fission Product Release from Fuel

    International Nuclear Information System (INIS)

    1989-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: The eighth OECD LOFT experiment was conducted on 7 March 1985. It was the second of the two experiments to be performed in the LOFT facility with intentional release of fission products. Its principal objectives were to determine the fission product release from the fuel during a severe fuel damage scenario and the subsequent transport of these fission products in a predominantly vapor/aerosol environment. This was the largest severe fuel damage experiment ever conducted, and serves as an important benchmark between smaller scale tests and the TMI-2 accident. 3 - Experimental limitations or shortcomings: Short core and steam generator, excessive core bypass, other scaling compromises, and lack of adequate measurements in certain areas

  10. Progress report on LOFT rake designs

    International Nuclear Information System (INIS)

    Bearden, R.G.

    1977-01-01

    Evaluation of data from Loss-of-Fluid Test (LOFT) nonnuclear tests has shown a need for profile measurements at several locations in the LOFT piping. A prototype rake consisting of three Drag-Disc Turbine Transducers (DTT) has been designed and fabricated for installation at one location (FE-BL-1) in the blowdown loop. After successful operation during a LOFT nonnuclear test (L1-4) scheduled for May, 1977, additional rakes will be installed in the primary and blowdown loops. A research program to develop a pitot tube rake for measurement of steady state and transient two-phase flows is in progress at McMaster University, Hamilton, Ontario. A rake of thermocouples and pitot tubes will be developed for installation near the emergency core coolant (ECC) injection points

  11. BEMUSE phase II report - Re-Analysis of the ISP-13 Exercise, Post Test Analysis of the LOFT L2-5 Test Calculation

    International Nuclear Information System (INIS)

    Petruzzi, A.; D'Auria, F.; Crecy, Agnes de; Bazin, P.; Borisov, S.; Skorek, T.; Glaeser, H.; Benoit, J. P.; Chojnacki, E.; Fujioka, K.; Inoue, S.; Chung, B.D.; Trosztel, I.; Toth, I.; Oh, D. Y.; Pernica, R.; Kyncl, M.; Macek, J.; Macian, R.; Tanker, E.; Soyer, A. E.; Ozdere, O.; Perez, M.; Reventos, F.

    2005-11-01

    The BEMUSE (Best Estimate Methods - Uncertainty and Sensitivity Evaluation) Programme is focused on applications of the uncertainty methodologies to Large Break LOCA scenarios. The main goals of the Programme are: - To evaluate the practicability, quality and reliability of best-estimate methods including uncertainty evaluations in applications relevant to nuclear reactor safety; - To develop common understanding; - To promote / facilitate their use by the regulator bodies and the industry. The scope of the Phase II of BEMUSE is to perform Large Break LOCA analysis making reference to the experimental data of LOFT L2-5 in order to address the issue of 'the capabilities of computational tools', including the scaling / uncertainty analysis. The operational objective of the activity is the quality demonstration of the system code calculations in performing LBLOCA analysis through the fulfilment of a comprehensive set of common criteria established in correspondence of different steps of the code assessment process. In particular criteria and threshold values for selected parameters have been adopted for: a) The developing of the nodalization; b) The evaluation of the steady state results; c) The qualitative and quantitative comparison between measured and calculated time trends. Main achievements of the Phase II, to be considered in the following phases of BEMUSE, are summarized as follows: - Almost all performed calculations appear qualified against the fixed criteria; - Dispersion bands of reference results appear substantially less than in ISP-13; - The sensitivity study shall be used as guidance for deriving the uncertainty bands in the following Phase III of the Programme

  12. Assessment of core damage models in SCDAP/RELAP5 during OECD LOFT LP-FP-2

    International Nuclear Information System (INIS)

    Coryell, E.W.

    1991-01-01

    The US Nuclear Regulatory Commission has sponsored a program to apply the SCDAP/RELAP5 code to analysis of the transient and reflood phases of the OECD LOFT LP-FP-2 Experiment. The principal objectives of the LP-FP-2 experiment were to determine the fission product release from the fuel during the early phases of a severe fuel damage scenario and to examine the phenomena controlling fission product transport in a vapor/aerosol environment. Calculations with the SCDAP/RELAP5 code, developed at the INEL with NRC support, have been performed to (1) examine the phenomena controlling the progression of both transient and reflood phases of the experiment, (2) enhance our understanding of the phenomena occurring during reflood and add credence to the postulated phenomenological sequence, (3) assess the ability of SCDAP/RELAP5 to examine severe fuel damage issues and phenomena, and (4) identify code strengths and deficiencies with the intent of prioritizing code improvements. Results indicate that the code is able to analyze the early phases of severe fuel damage reasonably well, with potential deficiencies in modelling interaction between molten control rod material and intact fuel

  13. Best-estimate analyses of LOFT anticipated transients with and without scram using DYNODE-P

    International Nuclear Information System (INIS)

    Kern, R.C.; Anderson, R.O.; Rautmann, D.A.

    1984-01-01

    Six LOFT transient tests with scram (L6-1, L6-2, L6-3, L6-7, L6-8B-1, and L6-8B-2) and two anticipated transient tests without scram (L9-3 and L9-4) have been analyzed using a best-estimate DYNODE-P/5.2 computer model. These tests span a wide range of anticipated operational occurrences for Pressurized Water Reactors. In general, satisfactory agreement between calculation and measurement for the key system parameters (nuclear power, primary and secondary pressures, temperatures, liquid levels, and flows) have been found. Sensitivity studies have resolved all significant discrepancies. These analyses have provided a significant qualification of the model for application to these types of events

  14. LOFCON-LOFT condenser program

    International Nuclear Information System (INIS)

    Lemmon, E.C.; MacKay, D.B.

    1978-01-01

    LOFCON is a program developed for the LOFT air condenser system contained in the secondary coolant system. Although the basic theory described herein is general, the program given is not--it is specifically for the LOFT configuration. LOFCON is presented in subroutine form so that it may be easily incorporated into a larger program describing the complete secondary side. Specifically LOFCON was written to be incorporated into the detailed CSMP model of the LOFT secondary coolant system simulation

  15. LOFT fuel rod surface temperature measurement testing

    International Nuclear Information System (INIS)

    Eaton, A.M.; Tolman, E.L.; Solbrig, C.W.

    1978-01-01

    Testing of the LOFT fuel rod cladding surface thermocouples has been performed to evaluate how accurately the LOFT thermocouples measure the cladding surface temperature during a loss-of-coolant accident (LOCA) sequence and what effect, if any, the thermocouple would have on core performance. Extensive testing has been done to characterize the thermocouple design. Thermal cycling and corrosion testing of the thermocouple weld design have provided an expected lifetime of 6000 hours when exposed to reactor coolant conditions of 620 K and 15.9 MPa and to sixteen thermal cycles with an initial temperature of 480 K and peak temperatures ranging from 870 to 1200K. Departure from nucleate boiling (DNB) tests have indicated a DNB penalty (5 to 28% lower) during steady state operation and negligible effects during LOCA blowdown caused by the LOFT fuel rod surface thermocouple arrangement. Experience with the thermocouple design in Power Burst Facility (PBF) and LOFT nonnuclear blowdown testing has been quite satisfactory. Tests discussed here were conducted using both stainless steel and zircaloy-clad electrically heated rod in the LOFT Test Support Facility (LTSF) blowdown simulation loop

  16. An assessment of uncertainty on a LOFT L2-5 LBLOCA PCT based on the ACE-RSM approach: complementary work for the OECD BEMUSE PHASE-III program

    International Nuclear Information System (INIS)

    Ahn, Kwang Il; Chung, Bub Dong; Lee, John C.

    2010-01-01

    As pointed out in the OECD BEMUSE Program, when a high computation time is taken to obtain the relevant output values of a complex physical model (or code), the number of statistical samples that must be evaluated through it is a critical factor for the sampling-based uncertainty analysis. Two alternative methods have been utilized to avoid the problem associated with the size of these statistical samples: one is based on Wilks' formula, which is based on simple random sampling, and the other is based on the conventional nonlinear regression approach. While both approaches provide a useful means for drawing conclusions on the resultant uncertainty with a limited number of code runs, there are also some unique corresponding limitations. For example, a conclusion based on the Wilks' formula can be highly affected by the sampled values themselves, while the conventional regression approach requires an a priori estimate on the functional forms of a regression model. The main objective of this paper is to assess the feasibility of the ACE-RSM approach as a complementary method to the Wilks' formula and the conventional regression-based uncertainty analysis. This feasibility was assessed through a practical application of the ACE-RSM approach to the LOFT L2-5 LBLOCA PCT uncertainty analysis, which was implemented as a part of the OECD BEMUSE Phase III program

  17. LOFT/LP-SB-2, Loss of Fluid Test, Small Hot Leg Break LOCA, Delayed Pump

    International Nuclear Information System (INIS)

    1989-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: The third OECD LOFT experiment was conducted on 14 July 1983. It simulated a 3-in (7.62 cm) equivalent break diameter located in the hot leg of the operating loop. The major objective of this experiment was to determine system transient characteristics for small hot leg break loss-of-coolant accidents with delayed pump trip. The experiment was conducted from initial temperature and pressure conditions representative of typical commercial PWRs. 3 - Experimental limitations or shortcomings: Short core and steam generator, excessive core bypass, other scaling compromises, and lack of adequate measurements in certain areas

  18. Prediction of thermal-Hydraulic phenomena in the LBLOCA experiment L2-3 using RELAP5/MOD2

    International Nuclear Information System (INIS)

    Bang, Young Seok; Chung, Bub Dong; Kim, Hho Jung

    1991-01-01

    The LOFT LOCE L2-3 was simulated using the RELAP5/MOD2 Cycle 36.04 code to assess its capability in predicting the thermal-hydraulic phenomena in LBLOCA of a PWR. The reactor vessel was simulated with two core channels and split downcomer modeling for a base case calculation using the frozen code. The result of the base calculation showed that the code predicted the hydraulic behavior, and the blowdown thermal response at high power region of the core reasonably and that the code had deficiencies in the critical flow model during subcooled-two-phase transition period, in the CHF correlation at high mass flux and in the blowdown rewet criteria. An overprediction of coolant inventory due to the deficiencies yielded the poor prediction of reflood thermal response. Improvement of the code, RELAP5/MOD2 Cycle 36.04, based on the sensitivity study increased the accuracy of the prediction of the rewet phenomena. (Author)

  19. LOFT Engineering Simulator

    International Nuclear Information System (INIS)

    Venhuizen, J.R.

    1982-02-01

    The LOFT Engineering Simulator was developed to supply plant equivalent data for evaluating graphic aids and advanced control concepts for nuclear plant operators. The Simulator, a combination of hardware and software, combines some of the features of best estimate (safety analysis) computer codes with reactor operator training simulators. The LOFT Engineering Simulator represents an attempt to develop a simulation with sufficient physical detail (solution of the conservation equations) for moderate accident simulation, but which will still run in real time and provide an interface for the operator to interact with the model. As a result of this combination, a real time simulation of the LOFT plant has been developed which yields realistic transient results. These data can be used for evaluating reactor control room aids such as Safety Parameter Displays and Janus Predictive Displays

  20. TRAC-PF1 analysis of LOFT steam-generator feedwater transient test L9-1

    International Nuclear Information System (INIS)

    Meier, J.K.

    1983-01-01

    The Transient Reactor Analysis Code (TRAC-PF1) calculations were compared to test data from Loss-of-Fluid Test (LOFT) L9-1, which was a loss-of-feedwater transient. This paper includes descriptions of the test and the TRAC input and compares the TRAC-calculated results with the test data. We conclude that the code predicted the experiment well, given the uncertainties in the boundary conditions. The analysis indicates the need to model all the flow paths and heat structures, and to improve the TRAC wall condensation heat-transfer model

  1. The LOFT Ground Segment

    DEFF Research Database (Denmark)

    Bozzo, E.; Antonelli, A.; Argan, A.

    2014-01-01

    targets per orbit (~90 minutes), providing roughly ~80 GB of proprietary data per day (the proprietary period will be 12 months). The WFM continuously monitors about 1/3 of the sky at a time and provides data for about ~100 sources a day, resulting in a total of ~20 GB of additional telemetry. The LOFT...... Burst alert System additionally identifies on-board bright impulsive events (e.g., Gamma-ray Bursts, GRBs) and broadcasts the corresponding position and trigger time to the ground using a dedicated system of ~15 VHF receivers. All WFM data are planned to be made public immediately. In this contribution...... we summarize the planned organization of the LOFT ground segment (GS), as established in the mission Yellow Book 1 . We describe the expected GS contributions from ESA and the LOFT consortium. A review is provided of the planned LOFT data products and the details of the data flow, archiving...

  2. Posttest REALP4 analysis of LOFT experiment L1-3A

    International Nuclear Information System (INIS)

    White, J.R.; Holmstrom, H.L.O.

    1977-10-01

    This report presents selected results of posttest RELAP4 modeling of LOFT loss-of-coolant experiment L1-3A, a double-ended isothermal cold leg break with lower plenum emergency core coolant injection. Comparisons are presented between the pretest prediction, the posttest analysis, and the experimental data. It is concluded that pressurizer modeling is important for accurately predicting system behavior during the initial portion of saturated blowdown. Using measured initial conditions rather than nominal specified initial conditions did not influence the system model results significantly. Using finer nodalization in the reactor vessel improved the prediction of the system pressure history by minimizing steam condensation effects. Unequal steam condensation between the downcomer and core volumes appear to cause the manometer oscillations observed in both the pretest and posttest RELAP4 analysis

  3. LOFT Augmented Operator Capability Program

    International Nuclear Information System (INIS)

    Hollenbeck, D.A.; Krantz, E.A.; Hunt, G.L.; Meyer, O.R.

    1980-01-01

    The outline of the LOFT Augmented Operator Capability Program is presented. This program utilizes the LOFT (Loss-of-Fluid Test) reactor facility which is located at the Idaho National Engineering Laboratory and the LOFT operational transient experiment series as a test bed for methods of enhancing the reactor operator's capability for safer operation. The design of an Operational Diagnotics and Display System is presented which was backfit to the existing data acquisition computers. Basic color-graphic displays of the process schematic and trend type are presented. In addition, displays were developed and are presented which represent safety state vector information. A task analysis method was applied to LOFT reactor operating procedures to test its usefulness in defining the operator's information needs and workload

  4. LOFT data reduction

    International Nuclear Information System (INIS)

    Norman, N.L.

    1975-08-01

    The Loss-of-Fluid Test (LOFT) Facility is an experimental facility built around a ''scaled'' version of a large pressurized water reactor (LPWR). LOFT will be used to run loss-of-coolant experiments (LOCEs) and to acquire the necessary data required ''to evaluate the adequacy and improve the analytical methods currently used to predict the loss-of-coolant accident (LOCA) response of LPWRs'' and ''to identify and investigate any unexpected event(s) or threshold(s) in the response of either the plant or the engineered safety features and develop analytical techniques that adequately describe and account for the unexpected behavior(s)''. During the LOCE this required data will be acquired and recorded in both analog and digital modes. Subsequent to the test the analog data will also be converted to the raw digital mode. This raw digital data will be converted to the desired engineering units using the LOFT Data Reduction System. This system is implemented on the IBM 360/75 and is a part of a commercially available data processing program called MAC/RAN III. The theory of reducing LOFT data to engineering units and the application of the MAC/ RAN III system to accomplish this reduction is given. (auth)

  5. LOFT fuel design and operating experience

    International Nuclear Information System (INIS)

    Russell, M.L.

    1978-01-01

    The purpose of the LOFT fuel is to provide a pressurized water reactor core that has (1) test instrumentation for measurement of core conditions and (2) materials and geometric features to ensure heat transfer, hydraulic, mechanical, chemical, metallurgical and nuclear behaviors are typical of large pressurized water reactors (LPWRS) during the loss-of-coolant accident (LOCA) sequence. The LOFT core is unique because it is designed for exposure to several LOCAs without loss of function

  6. LOFT system structural response during subcooled blowdown

    International Nuclear Information System (INIS)

    Martinell, J.S.

    1978-01-01

    The Loss-of-Fluid Test (LOFT) facility is a highly instrumented, pressurized water reactor test system designed to be representative of large pressurized water reactors (LPWRs) for the simulation of loss-of-coolant accidents (LOCAs). Detailed structural analysis and appropriate instrumentation (accelerometers and strain gages) on the LOFT system provided information for evaluation of the structural response of the LOFT facility for loss-of-coolant experiment (LOCE) induced loads. In general, the response of the system during subcooled blowdown was small with typical structural accelerations below 2.0 G's and dynamic strains less than 150 x 10 - 6 m/m. The accelerations measured at the steam generator and simulated steam generator flange exceeded LOCE design values; however, integration of the accelerometer data at these locations yielded displacements which were less than one half of the design values associated with a safe shutdown earthquake (SSE), which assures structural integrity for LOCE loads. The existing measurement system was adequate for evaluation of the LOFT system response during the LOCEs. The conditions affecting blowdown loads during nuclear LOCEs will be nearly the same as those experienced during the nonnuclear LOCEs, and the characteristics of the structural response data in both types of experiments are expected to be the same. The LOFT system is concluded to be adequately designed and further analysis of the LOFT system with structural codes is not required for future LOCE experiments

  7. LOFT fuel rod pressure measurement

    International Nuclear Information System (INIS)

    Billeter, T.R.

    1979-01-01

    Pressure sensors selected for measuring fuel rod pressure within the LOFT reactor exhibited stable, repeatable operating characteristics during calibrations at temperatures up to 800 0 F and pressures to 2500 psig. All sensors have a nominal sensitivity of .5 millivolts per psi, decreasing monotonically with temperature. Output signal increases linearly with increasing pressure up to 2000 psig. For imposed slow and rapid temperature variations and for pressure applied during these tests, the sensor indicates a pressure at variance with the actual value by up to 15% of reading. However, the imposed temperature rates of change often exceeded the value of -10 0 F/sec. specified for LOFT. The series of tests in an autoclave permit creation of an environment most closely resembling sensor operating conditions within LOFT. For multiple blowdowns and for longtime durations the sensor continued to provide pressure-related output signals. For temperature rates up to -87 0 F/sec, the indicated pressure measurement error remained less than 13% of reading. Adverse effects caused by heating the 1/16 inch O.D. signal cable to 800 0 F contributed only insignificantly to the noted pressure measurement error

  8. LOFT voltage insertion calibaration program

    International Nuclear Information System (INIS)

    Tillitt, D.N.; Miyasaki, F.S.

    1975-08-01

    The Loss-of-Fluid Test (LOFT) Facility is an experimental facility built around a ''scaled'' version of a large pressurized water reactor (LPWR). Part of this facility is the Data Acquisition and Visual Display System (DAVDS) as defined by the LOFT System Design Document SDD 1.4.2C. The DAVDS has a 702 data channel recording capability of which 548 are recorded digitally. The DAVDS also contains a Voltage Insertion Calibration Subsystem used to inject precise and known voltage steps into the recording systems. The computer program that controls the Voltage Insertion Calibration Subsystem is presented. 7 references. (auth)

  9. LOFT pressurizer safety: relief valve reliability

    Energy Technology Data Exchange (ETDEWEB)

    Brown, E.S.

    1978-01-18

    The LOFT pressurizer self-actuating safety-relief valves are constructed to the present state-of-the-art and should have reliability equivalent to the valves in use on PWR plants in the U.S. There have been no NRC incident reports on valve failures to lift that would challenge the Technical Specification Safety Limit. Fourteen valves have been reported as lifting a few percentage points outside the +-1% Tech. Spec. surveillance tolerance (9 valves tested over and 5 valves tested under specification). There have been no incident reports on failures to reseat. The LOFT surveillance program for assuring reliability is equivalent to nuclear industry practice.

  10. LOFT pressurizer safety: relief valve reliability

    International Nuclear Information System (INIS)

    Brown, E.S.

    1978-01-01

    The LOFT pressurizer self-actuating safety-relief valves are constructed to the present state-of-the-art and should have reliability equivalent to the valves in use on PWR plants in the U.S. There have been no NRC incident reports on valve failures to lift that would challenge the Technical Specification Safety Limit. Fourteen valves have been reported as lifting a few percentage points outside the +-1% Tech. Spec. surveillance tolerance (9 valves tested over and 5 valves tested under specification). There have been no incident reports on failures to reseat. The LOFT surveillance program for assuring reliability is equivalent to nuclear industry practice

  11. Core-power and decay-time limits for disabled automatic-actuation of LOFT ECCS

    International Nuclear Information System (INIS)

    Hanson, G.H.

    1978-01-01

    The Emergency Core Cooling System (ECCS) for the LOFT reactor may need to be disabled for modifications or repairs of hardware or instrumentation or for component testing during periods when the reactor system is hot and pressurized, or it may be desirable to enable the ECCS to be disabled without the necessity of cooling down and depressurizing the reactor. A policy involves disabling the automatic-actuation of the LOFT ECCS, but still retaining the manual actuation capability. Disabling of the automatic actuation can be safely utilized, without subjecting the fuel cladding to unacceptable temperatures, when the LOFT power decays to 33 kW; this power level permits a maximum delay of 20 minutes following a LOCA for the manual actuation of ECCS. For the operating power of the L2-2 Experiment, the required decay-periods (with operating periods of 40 and 2000 hours) are about 21 and 389 hours, respectively. With operating periods of 40 and 2000 hours at Core-I full power, the required decay-periods are about 42 and 973 hours, respectively. After these decay periods the automatic actuation of the LOFT ECCS can be disabled assuming a maximum delay of 20 minutes following a LOCA for the manual actuation of ECCS. The automatic and manual lineup of the ECCS may be waived if decay power is less than 11 kW

  12. Potential of LOFT telescope for the search of dark matter

    CERN Document Server

    Neronov, A; Iakubovskyi, D.; Ruchayskiy, O.

    2014-01-01

    Large Observatory For X-ray Timing (LOFT) is a next generation X-ray telescope selected by European Space Agency as one of the space mission concepts within the ``Cosmic Vision'' programme. The Large Area Detector on board of LOFT will be a collimator-type telescope with an unprecedentedly large collecting area of about 10 square meters in the energy band between 2 and 100 keV. We demonstrate that LOFT will be a powerful dark matter detector, suitable for the search of the X-ray line emission expected from decays of light dark matter particles in galactic halos. We show that LOFT will have sensitivity for dark matter line search more than an order of magnitude higher than that of all existing X-ray telescopes. In this way, LOFT will be able to provide a new insight into the fundamental problem of the nature of dark matter.

  13. Thermal analysis of LOFT modular DTT for LOCE transient

    International Nuclear Information System (INIS)

    Martin, C.M.

    1978-01-01

    A thermal analysis was performed on the LOFT modular drag-disc turbine transducer (MDTT) modular assembly. The purpose of this analysis was to determine the maximum temperature difference between the MDTT shroud and end cap during a LOCE. This temperature difference is needed for stress analysis of the MDTT endcap to fairing welds. The thermal analysis was done using TRIPLE, a three dimensional finite element code. A three dimensional model of the MDTT was made and transient temperature solutions were found for the different MDTT locations. The fluid temperature transients used for the solutions at all locations were from RELAP4 predictions of the LOFT L2-4 test which is considered the most severe temperature transient. Results of these calculations show the maximum temperature difference is 92 0 C (165 0 F) and occurs in the intact loop cold leg. This value and those found at other locations, are evaluated from the best available RELAP predicted temperatures during a nuclear LOCE

  14. Report of the LOFT special review group. Technical report

    International Nuclear Information System (INIS)

    Ross, D.F. Jr.

    1981-02-01

    This report represents the results of the LOFT Special Review Group (LSRG) evaluation of the LOFT program and is submitted to the Commission as an aid in its decision whether to continue NRC support of the LOFT project beyond FY 1982. The principal consensus reached by the LSRG recommends continued NRC support of the LOFT program through FY 1983

  15. LOFT fuel design and operating experience

    International Nuclear Information System (INIS)

    Russell, M.L.

    1979-01-01

    The objective of the LOFT fuel design and fabrication effort was to provide a pressurized water reactor core that has (1) materials and geometric features to ensure that heat transfer, hydraulic, mechanical, chemical, metallurgical and nuclear behaviors are typical of large pressurized water reactors (PWR) during the loss-of-coolant accident (LOCA) sequence and (2) test instrumentation for measurement of core conditions. The LOFT core is unique because it is designed for exposure to several LOCAs without loss of function. This paper summarizes the design effort and extent to which the design objectives have been achieved

  16. Experiment data report for Loft anticipated transient experiments 16-1, 16-2, and 16-3

    International Nuclear Information System (INIS)

    Batt, D.L.; Carpenter, J.M.

    1980-12-01

    This report presents uninterpreted experimental data from the second, third, and fourth anticipated transient experiments (Experiments L6-2, L6-1, and L6-3), conducted in the Loss-of-Fluid Test (LOFT) facility. Experiment L6-2 simulated a loss of forced primary coolant flow in a large PWR by tripping power to primary coolant pump motor generator sets, allowing the pumps to coast down under the influence of the flywheel system. Reactor scram initiated on indication of low flow in the primary coolant system (PCS). Experiment L6-1 simulated a loss of steam load in a large PWR by closing the steam flow control valve which reduced heat removal from the secondary coolant system and caused the PCS temperature and pressure to increase until reactor scram initiated on indication on high PCS pressure. Experiment L6-3 simulated an excessive load increase in a large PWR by opening the steam flow control valve at its maximum rate. PCS temperature and pressure decreased, causing the reactor to scram on indication of low PCS pressure. All experiments were complete when the plant was returned to a hot-standby condition

  17. Monitoring the Crab Nebula with LOFT

    Science.gov (United States)

    Wilson-Hodge, Colleen A.

    2012-01-01

    From 2008-2010, the Crab Nebula was found to decline by 7% in the 15-50 keV band, consistently in Fermi GBM, INTEGRAL IBIS, SPI, and JEMX, RXTE PCA, and Swift BAT. From 2001-2010, the 15-50 keV flux from the Crab Nebula typically varied by about 3.5% per year. Analysis of RXTE PCA data suggests possible spectral variations correlated with the flux variations. I will present estimates of the LOFT sensitivity to these variations. Prior to 2001 and since 2010, the observed flux variations have been much smaller. Monitoring the Crab with the LOFT WFM and LAD will provide precise measurements of flux variations in the Crab Nebula if it undergoes a similarly active episode.

  18. Loft over ydelser

    DEFF Research Database (Denmark)

    Krogh Graversen, Brian; Tinggaard, Karen

    I de seneste år er der gennemført en række ændringer i reglerne for beregningen af den økonomiske hjælp til kontanthjælpsmodtagere for at øge disses økonomiske incitament til at finde et arbejde. En af ændringerne er indførelsen af et loft over, hvor meget kontanthjælpsmodtagerne samlet kan modtage...... desuden på, hvordan den økonomiske situation for de berørte familier har ændret sig. Endelig belyser de, hvilke udfordringer og problemer kommunerne har haft med implementeringen af lovgivningen vedrørende loft over ydelser. Undersøgelsen er bestilt og finansieret af Arbejdsdirektoratet....

  19. Lofts: habitações para além do contexto moderno Lofts: habitaciones más allá del contexto moderno Lofts: habitations for beyond the modern scene

    Directory of Open Access Journals (Sweden)

    Bruno Ricardo Vasconcelos

    2012-01-01

    Full Text Available Este artigo problematiza a habitação humana na contemporaneidade, tomando o loft como objeto de análise. Seu objetivo é dialogar, a partir de perspectivas críticas acerca das implicações que emergem da re-significação do espaço da casa, lócus das relações interpessoais contemporâneas. A partir de um referencial teórico que transita entre a psicanálise e a Sociologia, constata-se que as dinâmicas que se dão num contexto de transição, onde os modos de vida estão em metamorfose, assim como o seu contorno e seu substrato. E nesse contexto, o loft emerge como um símbolo da pós-modernidade, na transposição do espaço público para o privado, sendo um local onde tudo se vê e tudo se mostra, da liquidez das relações e da solidão.Este artículo problematiza la vivienda humana en la contemporaneidad, tomando el loft como objeto de análisis. Su objetivo es dialogar, desde perspectivas críticas acerca de las implicaciones que emergen de la resignificación del espacio de la casa, locus de las relaciones interpersonales contemporáneas. Desde un referencial teórico que transita entre el Psicoanálisis y la Sociología, se constata que las dinámicas que se dan en un contexto de transición, donde los modos de vida están en metamorfosis, así como su contorno y su substrato. Y en ese contexto, el loft emerge como un símbolo de la post modernidad, en la transposición del espacio público para el privado, siendo un lugar donde todo se ve y todo se muestra, de la liquidez de las relaciones y de la soledad.This article discusses the human habitation in contemporary times, taking the loft as an object of analysis. Its objective is to dialogue, parting from critical perspectives about the implications that emerges of re-signification of the house's space, locus of the interpersonal contemporary relationships. Using theorical references that moves towards Psychoanalysis and Sociology, it is observed that the dynamics which develops

  20. OECD-LOFT large break LOCA experiments: phenomenology and computer code analyses

    International Nuclear Information System (INIS)

    Brittain, I.; Aksan, S.N.

    1990-08-01

    Large break LOCA data from LOFT are a very important part of the world database. This paper describes the two double-ended cold leg break tests LP-02-6 and LP-LB-1 carried out within the OECD-LOFT Programme. Tests in LOFT were the first to show the importance of both bottom-up and top-down quenching during blowdown in removing stored energy from the fuel. These phenomena are discussed in detail, together with the related topics of the thermal performance of nuclear fuel and its simulation by electric fuel rod simulators, and the accuracy of cladding external thermocouples. The LOFT data are particularly important in the validation of integral thermal-hydraulics codes such as TRAC and RELAP5. Several OECD partner countries contributed analyses of the large break tests. Results of these analyses are summarised and some conclusions drawn. 32 figs., 3 tabs., 45 refs

  1. LOFT: a nuclear plant providing realistic answers to PWR licensing issues

    Energy Technology Data Exchange (ETDEWEB)

    Solbrig, C.W.

    1980-01-01

    The following topics are discussed: the LOFT mission; LOFT results which have been used by NRC's office of Nuclear Reactor Regulation (NRR); NRR current LOFT information needs; and the channels of LOFT results dissemination.

  2. Recalculation of loads on LOFT penetrations 1A, 2A, 3E, 3F, 5A, 5B, 5C, 5D, 5E, 5F, 7A, 9A, 11C, 17A, 17B, 20A, 20B, 20C, 21A

    International Nuclear Information System (INIS)

    McFadden, D.F.

    1978-01-01

    The loads on the piping nozzles penetrating the LOFT containment vessel are presented. Since the design and construction of the containment vessel, piping systems that penetrate the nozzles have been changed. Consequently, the moments and forces on the containment vessel are different than those stated in LOFT specification S-1. Two combined load cases were tabulated, deadweight plus thermal expansion plus operating basis earthquake and deadweight plus safe shutdown earthquake

  3. LOFT/LP-02-6, Loss of Fluid Test, 1. OECD Large Break Experiment

    International Nuclear Information System (INIS)

    1989-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: The fourth OECD LOFT experiment was conducted on 3 October 1983. This was the first OECD LOFT large break experiment. The initial and boundary conditions were chosen to be representative of USNRC licensing limits for commercial PWRs. This included loss of off-site power coincident with LOCA initiation. This experiment included the first use in LOFT of pressurized fuel rods in the center bundle. The experiment was initiated by opening the quick-opening blow-down valves in the broken hot and cold legs. 3 - Experimental limitations or shortcomings: Short core and steam generator, excessive core bypass, other scaling compromises, and lack of adequate measurements in certain areas

  4. LOFT instrumented fuel design and operating experience

    International Nuclear Information System (INIS)

    Russell, M.L.

    1979-01-01

    A summary description of the Loss-of-Fluid Test (LOFT) system instrumented core construction details and operating experience through reactor startup and loss-of-coolant experiment (LOCE) operations performed to date are discussed. The discussion includes details of the test instrumentation attachment to the fuel assembly, the structural response of the fuel modules to the forces generated by a double-ended break of a pressurized water reactor (PWR) coolant pipe at the inlet to the reactor vessel, the durability of the LOFT fuel and test instrumentation, and the plans for incorporation of improved fuel assembly test instrumentation features in the LOFT core

  5. Results of recent LOFT experiments

    International Nuclear Information System (INIS)

    Leach, L.P.; Hanson, D.J.; Batt, D.L.

    1982-01-01

    Five experiments were performed in the Loss-of-Fluid Test (LOFT) facility during the past year. The experiments conducted spanned a wide range of potential accident scenarios, including large and small break loss-of-coolant accidents (LOCAs), control rod withdrawal accidents, uncontrolled boron dilution, and anticipated transients without scram (ATWS). This summary describes these experiments and presents results available from the experiments and experiment prediction calculations. A brief overview is given for the remaining experiment planned in the LOFT Program

  6. LOFT/LP-SB-3, Loss of Fluid Test, Cold Leg Break LOCA, No High Pressure injection System (HPIS)

    International Nuclear Information System (INIS)

    1989-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: The sixth OECD LOFT experiment was conducted on 5 March 1984. It simulated a 1.8-in cold leg break LOCA with no HPIS available. This experiment was designed mainly for investigation of plant recovery effectiveness using secondary bleed and feed during core uncover and addressed accumulator injection at low pressure differentials. 3 - Experimental limitations or shortcomings: Short core and steam generator, excessive core bypass, other scaling compromises, and lack of adequate measurements in certain areas

  7. ISR-1 Seminar: Nuclear Cloud Lofting

    International Nuclear Information System (INIS)

    Walker, Andrew Charles

    2016-01-01

    This report goes into detail about motivation, background, lofting methodologies (empirical, parcel methods, Navier-Stokes), DELFIC Model, validation, DIORAMA integration, and has the following conclusions: A parcel methodology was applied to develop the DIORAMA cloud lofting module. It is based on the DELFIC model and treats the cloud as a homogenous unit. It solves the set of 8 ODEs for cloud properties. It outputs the time history of the cloud height, radius, and other parameters. The cloud lofting module was tuned with 54 test cases. An iterative brute force search was carried out to find the best fit tuning parameters. The best fit parameters yielded average cloud height errors of 12.9% and 10.3% for the bottom and top, respectively. This all allows for more accurate modeling of the propagation of delayed gamma rays in DIORAMA.

  8. Multidimensional analysis of fluid flow in the loft cold leg blowdown pipe during a loss-of-coolant experiment

    International Nuclear Information System (INIS)

    Demmie, P.N.; Hofmann, K.R.

    1979-03-01

    A computer analysis of fluid flow in the Loss-of-Fluid Test (LOFT) cold leg blowdown pipe during a loss-of-coolant experiment (LOCE) was performed using the computer program K-FIX/MOD1. The purpose of this analysis was to evaluate the capability of K-FIX/MOD1 to calculate theoretical fluid quantity distributions in the blowdown pipe during a LOCE for possible application to the analysis of LOFT experimental data, the determination of mass flow, or the development of data reduction models. A rectangular section of a portion of the LOFT blowdown pipe containing measurement Station BL-1 was modeled using time-dependent boundary conditions. Fluid quantities were calculated during a simulation of the first 26 s of LOFT LOCE L1-4. Sensitivity studies were made to determine changes in void fractions and velocities resulting from specific changes in the inflow boundary conditions used for this simulation

  9. LOFT/LP-FP-1B, Loss of Fluid Test, Fission Product Release Experiment

    International Nuclear Information System (INIS)

    1989-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: The seventh OECD LOFT experiment was conducted on 19 December 1984. It was the first of the two experiments to be performed in the LOFT facility with intentional release of fission products. Its objectives were to obtain data on fission product release from the fuel-cladding gap into vapor and reflood water and to collect data on transport of these fission products through and out of the reactor coolant system. The experiment was initiated by a reactor scram with one second delayed opening of the quick-opening blowdown valves. 3 - Experimental limitations or shortcomings: Short core and steam generator, excessive core bypass, other scaling compromises, and lack of adequate measurements in certain areas

  10. Sensitivity analysis for CORSOR models simulating fission product release in LOFT-LP-FP-2 severe accident experiment

    Energy Technology Data Exchange (ETDEWEB)

    Hoseyni, Seyed Mohsen [Islamic Azad Univ., Tehran (Iran, Islamic Republic of). Dept. of Basic Sciences; Islamic Azad Univ., Tehran (Iran, Islamic Republic of). Young Researchers and Elite Club; Pourgol-Mohammad, Mohammad [Sahand Univ. of Technology, Tabriz (Iran, Islamic Republic of). Dept. of Mechanical Engineering; Yousefpour, Faramarz [Nuclear Science and Technology Research Institute, Tehran (Iran, Islamic Republic of)

    2017-03-15

    This paper deals with simulation, sensitivity and uncertainty analysis of LP-FP-2 experiment of LOFT test facility. The test facility simulates the major components and system response of a pressurized water reactor during a LOCA. MELCOR code is used for predicting the fission product release from the core fuel elements in LOFT LP-FP-2 experiment. Moreover, sensitivity and uncertainty analysis is performed for different CORSOR models simulating release of fission products in severe accident calculations for nuclear power plants. The calculated values for the fission product release are compared under different modeling options to the experimental data available from the experiment. In conclusion, the performance of 8 CORSOR modeling options is assessed for available modeling alternatives in the code structure.

  11. Code comparison results for the loft LP-FP-2 experiment

    International Nuclear Information System (INIS)

    Merilo, M.; Mecham, D.C.

    1991-01-01

    Computer code calculations are compared with thermal hydraulic and fission product release, transport, and deposition data obtained from the OECD-LOFT LP-FP-2 experiment. Except for the MAAP code, which is a fully integrated severe accident code, the thermalhydraulic and fission product behavior were calculated with different codes. Six organizations participated in the thermal hydraulic portion of the code comparison exercise. These calculations were performed with RELAP 5, SCDAP/RELAP 5, and MAAP. The comparisons show generally well developed capabilities to determine the thermal-hydraulic conditions during the early stages of a severe core damage accident. Four participants submitted detailed fission product behavior calculations. Except for MAAP, as stated previously, the fission product inventory, core damage, fission product release, transport and deposition were calculated independently with different codes. Much larger differences than observed for the thermalhydraulic comparison were evident. The fission product inventory calculations were generally in good agreement with each other. Large differences were observed for release fractions and amounts of deposition. Net release calculations from the primary system were generally accurate within a factor of two or three for the more important fission products

  12. LOFT uncertainty-analysis methodology

    International Nuclear Information System (INIS)

    Lassahn, G.D.

    1983-01-01

    The methodology used for uncertainty analyses of measurements in the Loss-of-Fluid Test (LOFT) nuclear-reactor-safety research program is described and compared with other methodologies established for performing uncertainty analyses

  13. LOFT uncertainty-analysis methodology

    International Nuclear Information System (INIS)

    Lassahn, G.D.

    1983-01-01

    The methodology used for uncertainty analyses of measurements in the Loss-of-Fluid Test (LOFT) nuclear reactor safety research program is described and compared with other methodologies established for performing uncertainty analyses

  14. Atmospheric Propagation Modeling Indicates Homing Pigeons use Loft-Specific Infrasonic 'Map' Cues

    Science.gov (United States)

    Hagstrum, J. T.; Baker, L. M.; Spritzer, J. M.; McKenna, M. H.

    2011-12-01

    Pigeons (Columba livia) released at distant sites commonly depart in directions significantly off the actual homeward bearing. Such site-dependent deviations, or biases, for birds from a given loft are generally stable over time, but can also change from hour to hour, day to day, and year to year. At some release sites, birds consistently vanish in random directions and have longer flight times and lower return rates. Release sites characterized by frequent disorientation are not uncommon for pigeon lofts in both Europe and the USA. One such site is the Jersey Hill fire tower in upstate New York located ~120 km W of the Cornell loft in Ithaca. Cornell birds released at Jersey Hill between 1968 and 1987 almost always vanished randomly, although birds from other lofts had little difficulty orienting there. The results for one day, however, stand out: on August 13, 1969, Cornell birds released at Jersey Hill vanished consistently to the NE (r = 0.921; n=7) and returned home after normal flight times. Cornell pigeons released the next day again showed 'normal' behavior for the site and departed randomly. If, in fact, the birds are using acoustic cues to navigate, the long-term acoustic 'dead' zone we propose for Jersey Hill, due to prevailing atmospheric conditions, indicates that the cues are coming from a single, relatively restricted area, most likely surrounding the home loft. We have modeled the transmission of infrasonic waves, presumably coupled to the atmosphere from ocean-generated microseisms (0.14 Hz), between the Cornell loft and a number of release sites using HARPA (Hamiltonian Acoustic Ray-tracing Program for the Atmosphere) and rawinsonde data collected near Albany and Buffalo, NY. The HARPA modeling shows that acoustic signals from the Cornell loft reached Jersey Hill only on a few release days with unusual atmospheric conditions, including August 13, and were launched at angles less than ~2° above horizontal, most likely from steep-sided terrain in

  15. LOFT instrumentation

    International Nuclear Information System (INIS)

    Bixby, W.W.

    1979-01-01

    A description of instrumentation used in the Loss-of-Fluid Test (LOFT) large break Loss-of-Coolant Experiments is presented. Emphasis is placed on hydraulic and thermal measurements in the primary system piping and components, reactor vessel, and pressure suppression system. In addition, instrumentation which is being considered for measurement of phenomena during future small break testing is discussed. (orig.) 891 HP/orig. 892 BRE [de

  16. Scaling and instrumentation of the LOFT facility

    International Nuclear Information System (INIS)

    Modro, S.M.; Goodrich, L.D.; McPherson, G.D.

    1985-01-01

    This paper describes the LOFT experimental facility and instrumentation of the facility during small break loss-of-coolant experiments. Basic scaling considerations applied in the facility design are presented. Because LOFT was not designed with emphasis on small break LOCA some atypicalities with regard to small break transients are discussed. Review of important small break LOCA phenomena observed during the experiments and their measurability is provided

  17. The LOFT perspective on neutron star thermonuclear bursts

    DEFF Research Database (Denmark)

    in ’t Zand, J.J.M.; Altamirano, D.; Ballantyne, D. R.

    This is a White Paper in support of the mission concept of the Large Observatory for X-ray Timing (LOFT), proposed as a medium-sized ESA mission. We discuss the potential of LOFT for the study of thermonuclear X-ray bursts on accreting neutron stars. For a summary, we refer to the paper....

  18. LOFT - The large observatory for x-ray timing

    DEFF Research Database (Denmark)

    Feroci, M.; Den Herder, J.W.; Argan, A.

    2012-01-01

    The LOFT mission concept is one of four candidates selected by ESA for the M3 launch opportunity as Medium Size missions of the Cosmic Vision programme. The launch window is currently planned for between 2022 and 2024. LOFT is designed to exploit the diagnostics of rapid X-ray flux and spectral v...

  19. PWR fuel behavior: lessons learned from LOFT

    International Nuclear Information System (INIS)

    Russell, M.L.

    1981-01-01

    A summary of the experience with the Loss-of-Fluid Test (LOFT) fuel during loss-of-coolant experiments (LOCEs), operational and overpower transient tests and steady-state operation is presented. LOFT provides unique capabilities for obtaining pressurized water reactor (PWR) fuel behavior information because it features the representative thermal-hydraulic conditions which control fuel behavior during transient conditions and an elaborate measurement system to record the history of the fuel behavior

  20. NEPTUN/5052, PWR LOCA Cooling Heat Transfer Tests for Loft, Reflood Test

    International Nuclear Information System (INIS)

    Richner, M.; Analytis, G.Th.; Aksan, S.N.

    1993-01-01

    1 - Description of test facility: NEPTUN is designed to perform PWR LOCA simulation experiments, which provide the full length emergency cooling heat transfer tests for LOFT. Therefore the NEPTUN heater bundle with 33 electrical heater elements and 4 guide tubes simulates a section of the LOFT nuclear core. The main test loop also contains measuring systems for the carry-over rate and for the steam expelled, and a back-pressure control system. A water loop brings the water to the initial reflooding conditions. In addition, auxiliary systems maintain normal operating conditions. 2 - Description of test: Test 5052 is one of a series of 40 reflood tests performed in NEPTUN. Before the start of the test, the flooding water in its circuit is brought to the following conditions: pressure = 4.1 bar; velocity = 2.5 cm/sec; subcooling temperature = 78 C; single rod power = 2.45 kW; maximal initial cladding temperature = 867 C. 3 - Status: CSNI1013/01, 21-Jul-1993 Arrived at NEADB

  1. Defect analysis program for LOFT. Progress report, 1977

    International Nuclear Information System (INIS)

    Doyle, R.E.; Scoonover, T.M.

    1978-03-01

    In order to alleviate problems encountered while performing previous defect analyses on components of the LOFT system, regions of LOFT most likely to require defect analysis have been identified. A review of available documentation has been conducted to identify shapes, sizes, materials, and welding procedures and to compile mechanical property data. The LOFT Reactor Vessel Material Surveillance Program has also been reviewed, and a survey of available literature describing existing techniques for conducting elastic-plastic defect analysis was initiated. While large amounts of mechanical property data were obtained from the available documentation and the literature, much information was not available, especially for weld heat-affected zones. Therefore, a program of mechanical property testing is recommended for FY-78 as well as continued literature search. It is also recommended that fatigue-crack growth-rate data be sought from the literature and that evaluation of the various techniques of elastic-plastic defect analysis be continued. Review of additional regions of the LOFT system in the context of potential defect analysis will be conducted as time permits

  2. LOFT advanced control room operator diagnostic and display system (ODDS)

    International Nuclear Information System (INIS)

    Larsen, D.G.; Robb, T.C.

    1980-01-01

    The Loss-of-Fluid Test (LOFT) Reactor Facility in Idaho includes a highly instrumented nuclear reactor operated by the Department of Energy for the purpose of establishing nuclear safety requirements. The results of the development and installation into LOFT of an Operator Diagnostic and Display System (ODDS) are presented. The ODDS is a computer-based graphics display system centered around a PRIME 550 computer with several RAMTEK color graphic display units located within the control room and available to the reactor operators. Use of computer-based color graphics to aid the reactor operator is discussed. A detailed hardware description of the LOFT data system and the ODDS is presented. Methods and problems of backfitting the ODDS equipment into the LOFT plant are discussed

  3. The Science Payload of the LOFT Mission

    DEFF Research Database (Denmark)

    Feroci, Marco; den Herder, J.; van der Klis, M.

    The scientific payload onboard the Large Observatory For x-ray Timing mission (LOFT, see presentation by P. Ray et al. at this meeting) is composed of two instruments, the Large Area Detector (LAD, 10 m2 effective area in the primary energy range 2-30 keV, 1-deg collimated field of view) and the ...

  4. Displacement sensor for measurement of fuel rod elongation in the LOFT reactors

    International Nuclear Information System (INIS)

    Billeter, T.R.

    1979-09-01

    Qualification tests conducted for a period of 700 hours of each of three displacement measuring (LVDT) sensors confirmed applicability of the design for use in the Loss-of-Fluid-Test (LOFT) reactor. Operationally, the sensor satisfies all specified requirements for LOFT. Even for imposed temperature transients at rates up to 100 0 F/s, the indicated displacement remained within the allowed maximum error band of +- 10% of reading. The 0.6-inch O.D. by 5.5-inch long sensor exhibited a linearly related signal output variation for displacement variations of up to 1-inch range. Long term operation at temperatures of 100 0 F to 800 0 F caused no perceptible permanent change of operating characteristics

  5. Experiment data report for semiscale Mod-1 Test S-06-5. (LOFT counterpart test)

    International Nuclear Information System (INIS)

    1977-06-01

    Recorded test data are presented for Test S-06-5 of the Semiscale Mod-1 LOFT counterpart test series. These tests are among several Semiscale Mod-1 experiments conducted to investigate the thermal and hydraulic phenomena accompanying a hypothesized loss-of-coolant accident in a pressurized water reactor (PWR) system. Test S-06-5 was conducted from initial conditions of 2272 psia and 536 0 F to investigate the response of the Semiscale Mod-1 system to a depressurization and reflood transient following a simulated double-ended offset shear of the broken loop cold leg piping. During the test, cooling water was injected into the cold legs of the intact and broken loops to simulate emergency core coolant injection in a PWR. The purpose of Test S-06-5 was to assess the influence of the break nozzle geometry on core thermal and system response and on the subcooled and low quality mass flow rates at the break locations

  6. LOFT/LP-LB-1, Loss of Fluid Test, Large-Break LOCA Experiment

    International Nuclear Information System (INIS)

    1989-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, Thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCE is expected to closely model a LPWR LOCA. 2 - Description of test: Experiment LP-LB-1 was conducted on 3 February 1984 in the Loss-of-Fluid Test (LOFT) facility at the Idaho National Engineering Laboratory under the auspices of the Organization for Economic Cooperation and Development. The primary objectives of Experiment LP-LB-1 were to determine system transient characteristics and to assess code predictive capabilities for design basis large-break loss-of-coolant accidents in pressurized water reactors (PWRs). This experiment simulated a double-ended offset shear of one inlet pipe in a four-loop PWR and was initiated from conditions representative of licensing limits in a PWR. Other boundary conditions for the simulation were loss of offsite power, rapid primary coolant pump coast down, and United Kingdom minimum safeguard emergency core coolant injection rates. The nuclear fuel rods were not pressurized. The transient was initiated by opening the quick-opening blowdown valves in the broken loop hot and cold legs. 3 - Experimental limitations or shortcomings: Short core and steam generator, excessive core bypass, other scaling compromises, and lack of adequate measurements in certain areas

  7. Loft fire protection

    International Nuclear Information System (INIS)

    White, E.R.; Jensen, J.D.

    1980-01-01

    Quantified criteria that was developed and applied to provide in-depth fire protection for the Loss of Fluid Test (LOFT) Facility are presented. The presentation describes the evolution process that elevated the facility's fire protection from minimal to that required for a highly protected risk or improved risk. Explored are some infrequently used fire protection measures that are poorly understood outside the fire protection profession

  8. Prediction of LOFT core fluid conditions during blowdown and refill

    International Nuclear Information System (INIS)

    Grush, W.H.; White, J.R.

    1978-01-01

    One of the primary objectives of the LOFT (Loss-of-Fluid Test) Program is to provide data required to evaluate and improve the analytical methods currently used to predict the LOCA (Loss-of-Coolant Accident) response of large pressurized water reactors. The purpose of the paper is to describe the computer modeling methods used in predicting the fluid conditions in the LOFT core during the blowdown and refill phases of a nuclear LOCE (Loss-of-Coolant Experiment). Prediction results for a LOFT nonnuclear isothermal LOCE are compared to the experimental data to illustrate the validity of the modeling choices

  9. TRAC-PD2 modeling of LOFT and PWR small cold-leg breaks

    International Nuclear Information System (INIS)

    Knight, T.D.; Willcutt, G.J.E. Jr.; Lime, J.F.

    1981-01-01

    The Transient Reactor Analysis Code (TRAC) is being developed at the Los Alamos National Laboratory to provide advanced best-estimate predictions of postulated accidents in light-water reactors. TRAC-PD2, the latest publicly released version of the code, is currently being tested against small-break and other transients in experimental facilities; it is also being used to analyze postulated accidents in commercial power reactors. Calculated results for LOFT small-break experiments are compared to data, and the results from two small-break calculations for two different reactor systems are presented. It is concluded that TRAC-PD2 is useful for the analysis of cold-leg small-break accidents

  10. LOFT/LP-FW-1, Loss of Fluid Test, PWR Response to Loss-of-Feedwater Transient

    International Nuclear Information System (INIS)

    1989-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: The first OECD LOFT experiment was conducted on February 20, 1983. It was designed to evaluate the generic PWR system response during a complete loss-of-feedwater transient. The objective of the experiment was to investigate the performance of primary 'feed and bleed' using a 'bleed' from the PORV and 'feed' from the HPIS to provide decay heat removal and system pressure reduction while maintaining the primary coolant inventory. 3 - Experimental limitations or shortcomings: Short core and steam generator, excessive core bypass, other scaling compromises, and lack of adequate measurements in certain areas

  11. LOFT/LP-SB-1, Loss of Fluid Test, Small Hot Leg Break LOCA, Early Pump

    International Nuclear Information System (INIS)

    1989-01-01

    1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: The second OECD LOFT experiment was conducted on 23 June 1983. It simulated a 3-in (7.62 cm) equivalent break diameter located in the hot leg of the operating loop. The major objective of this experiment was to determine system transient characteristics for small hot leg break loss-of-coolant accidents with early pump trip. The experiment was conducted from initial temperature and pressure conditions representative of typical commercial PWRs. 3 - Experimental limitations or shortcomings: Short core and steam generator, excessive core bypass, other scaling compromises, and lack of adequate measurements in certain areas

  12. The LOFT (Large Observatory for X-ray Timing) background simulations

    DEFF Research Database (Denmark)

    Campana, R.; Feroci, M.; Del Monte, E.

    2012-01-01

    The Large Observatory For X-ray Timing (LOFT) is an innovative medium-class mission selected for an assessment phase in the framework of the ESA M3 Cosmic Vision call. LOFT is intended to answer fundamental questions about the behavior of matter in theh very strong gravitational and magnetic fields...

  13. Overview of LOFT instrumentation

    International Nuclear Information System (INIS)

    Bixby, W.W.

    1979-01-01

    A description of instrumentation used in the Loss-of-Fluid Test (LOFT) large break Loss-of-Coolant Experiments is presented. Emphasis is placed on hydraulic and thermal measurements in the primary system piping and components, reactor vessel, and pressure suppression system. In addition, instrumentation which is being considered for measurement of phenomena during future small break testing is discussed

  14. N-[(2S-4-Chloro-2-(l-menthyloxy-5-oxo-2,5-dihydro-3-furyl]-l-alanine

    Directory of Open Access Journals (Sweden)

    Kai Yang

    2009-05-01

    Full Text Available The title compound, C17H26ClNO5, was prepared via a tandem asymmetric Michael addition–elimination reaction of (5S-3,4-dichloro-5-(l-menthyloxyfuran-2(5H-one and l-alanine in the presence of potassium hydroxide. The five-membered furanone ring is approximately planar while the six-membered menthyloxy ring adopts a chair conformation. The crystal packing is stabilized by intermolecular O—H...O and N—H...O hydrogen bonds.

  15. LOFT fuel module structural response during loss-of-coolant experiments

    International Nuclear Information System (INIS)

    Saffell, B.F. Jr.; Selcho, H.S.

    1979-01-01

    The structural response of the reactor fuel modules installed in the Loss-of-Fluid Test (LOFT) facility have been analyzed for subcooled blowdown loading conditions associated with loss-of-coolant experiments (LOCE). Three independent analyses using the WHAM, SHOCK, and SAP computer codes have been interfaced to calculate the transient mechanical behavior of the LOFT fuel. Test data from two LOCEs indicate the analysis method is conservative. Structural integrity of the fuel modules has been assessed by monitoring guide tube temperatures and control rod drop times during the LOCEs. The analysis and experimental test data indicate the fuel module structural integrity will be maintained for the duration of the LOFT experimental program

  16. LOFT diesel generator ''A'' exhaust stack seismic analysis

    International Nuclear Information System (INIS)

    Blandford, R.K.

    1978-01-01

    A stress analysis of the LOFT Diesel Generator ''A'' Exhaust Stack was performed to determine its reaction to Safe-Shutdown Earthquake loads. The exhaust stack silencer and supporting foundation was found to be inadequate for the postulated seismic accelerations. Lateral support is required to prevent overturning of the silencer pedestal and reinforcement of the 4'' x 0.5'' silencer base straps is necessary. Basic requirements for this additional support are discussed

  17. Transient two-phase performance of LOFT reactor coolant pumps

    International Nuclear Information System (INIS)

    Chen, T.H.; Modro, S.M.

    1983-01-01

    Performance characteristics of Loss-of-Fluid Test (LOFT) reactor coolant pumps under transient two-phase flow conditions were obtained based on the analysis of two large and small break loss-of-coolant experiments conducted at the LOFT facility. Emphasis is placed on the evaluation of the transient two-phase flow effects on the LOFT reactor coolant pump performance during the first quadrant operation. The measured pump characteristics are presented as functions of pump void fraction which was determined based on the measured density. The calculated pump characteristics such as pump head, torque (or hydraulic torque), and efficiency are also determined as functions of pump void fractions. The importance of accurate modeling of the reactor coolant pump performance under two-phase conditions is addressed. The analytical pump model, currently used in most reactor analysis codes to predict transient two-phase pump behavior, is assessed

  18. Comparison of LOFT zero power physics testing measurement results with predicted values

    International Nuclear Information System (INIS)

    Rushton, B.L.; Howe, T.M.

    1978-01-01

    The results of zero power physics testing measurements in LOFT have been evaluated to assess the adequacy of the physics data used in the safety analyses performed for the LOFT FSAR and Technical Specifications. Comparisons of measured data with computed data were made for control rod worths, temperature coefficients, boron worths, and pressure coefficients. Measured boron concentrations at exact critical points were compared with predicted concentrations. Based on these comparisons, the reactivity parameter values used in the LOFT safety analyses were assessed for conservatism

  19. Analysis of core physics and thermal-hydraulics results of control rod withdrawal experiments in the LOFT facility

    International Nuclear Information System (INIS)

    Varacalle, D.J. Jr.; Chen, T.H.; Harvego, E.A.; Ollikkala, H.

    1983-01-01

    Two anticipated transient experiments simulating an uncontrolled control rod withdrawal event in a pressurized water reactor (PWR) were conducted in the Loss-of-Fluid Test (LOFT) Facility at the Idaho National Engineering Laboratory. The scaled LOFT 50-MW(t) PWR includes most of the principal features of larger commercial PWRs. The experiments tested the ability of reactor analysis codes to accurately calculate core reactor physics and thermal-hydraulic phenomena in an integral reactor system. The initial conditions and scaled operating parameters for the experiments were representative of those expected in a commercial PWR. In both experiments, all four LOFT control rod assemblies were withdrawn at a reactor power of 37.5 MW and a system pressure of 14.8 MPa

  20. Anticipated transient without SCRAM experiments at LOFT

    International Nuclear Information System (INIS)

    Grush, W.H.; Harvego, E.A.; Koizumi, Y.; Varacalle, D.J.

    1983-01-01

    This paper discusses the experimental results for two anticipated transients without scram (ATWS) experiments, and compares computer code predictions with the experimental data. Experiment L9-3 simulated an ATWS in a commercial pressurized water reactor (PWR) initiated by a complete loss of feedwater and Experiment L9-4 simulated a loss-of-offsite-power-initiated (loss of feedwater and trip of the primary coolant pumps) ATWS. The LOFT facility is uniquely suited for ATWS experiments because it is a volumetrically scaled (1/44) experimental PWR designed to simulate the major components and system responses of larger commercial PWRs during both hypothesized loss-of-coolant accidents and anticipated transients. In both of the examined experiments, the primary system transient behavior was dominated by the interactions between the steam generator primary-to-secondary heat removal, the reactor kinetics, and the relief valve actuation. It is demonstrated that the discussed ATWS events can be controlled by properly sized automatic safety systems

  1. Special LOFT features for improved monitoring and survival of LOCA transients

    International Nuclear Information System (INIS)

    Goodrich, L.D.; Leach, L.P.; Klingler, T.B.; Morrow, J.C.; Phoenix, W.C.; Satterwhite, D.G.; Sumpter, K.C.; Rouhani, S.Z.; Welland, H.J.

    1980-01-01

    LOFT is designed to monitor and survive Loss-Of-Coolant-Accidents (LOCAs). This report presents the primary design difference from LPWRs that were required to accomplish this. These design differences may be of interest to the nuclear power generator industry. This report should be revised semi-annually or as developments in the LOFT Program require

  2. A review of analyses of LOFT and semiscale tests made at IDAHO National Engineering Laboratory using RELAP5/MOD1

    International Nuclear Information System (INIS)

    Hall, P.C.

    1984-03-01

    Within the LOFT and Semiscale programs at INEL, many post-test analysis calculations have been performed using RELAP5/MOD1. In this report, these calculations are reviewed from the standpoint of assessing the performance of the code. Because the calculations were spread over a number of years, different cycles of RELAP5/MOD1 have been employed. Rather than explicitly assessing several cycles of the code, a more general view has been adopted and an attempt has been made to identify those areas in which the code is systematically successful or alternatively, frequently experiences difficulties. (author)

  3. Core-power and decay-time limits for disabled automatic-actuation of LOFT ECCS

    International Nuclear Information System (INIS)

    Hanson, G.H.

    1978-01-01

    The Emergency Core Cooling System (ECCS) for the LOFT reactor may need to be disabled for modifications or repairs of hardware or instrumentation or for component testing during periods when the reactor system is hot and pressurized, or it may be desirable to enable the ECCS to be disabled without the necessity of cooling down and depressurizing the reactor. LTR 113-47 has shown that the LOFT ECCS can be safely bypassed or disabled when the total core power does not exceed 25 kW. A modified policy involves disabling the automatic actuation of the LOFT ECCS, but still retaining the manual activation capability. Disabling of the automatic actuation can be safely utilized, without subjecting the fuel cladding to unacceptable temperatures, when the LOFT power decays to 70 kW; this power level permits a maximum delay of 20 minutes following a LOCA for the manual actuation of ECCS

  4. LOFT integral test system final safety analysis report

    International Nuclear Information System (INIS)

    1974-03-01

    Safety analyses are presented for the following LOFT Reactor systems: engineering safety features; support buildings and facilities; instrumentation and controls; electrical systems; and auxiliary systems. (JWR)

  5. LOFT differential pressure uncertainty analysis

    International Nuclear Information System (INIS)

    Evans, R.P.; Biladeau, G.L.; Quinn, P.A.

    1977-03-01

    A performance analysis of the LOFT differential pressure (ΔP) measurement is presented. Along with completed descriptions of test programs and theoretical studies that have been conducted on the ΔP, specific sources of measurement uncertainty are identified, quantified, and combined to provide an assessment of the ability of this measurement to satisfy the SDD 1.4.1C (June 1975) requirement of measurement of differential pressure

  6. The LOFT wide field monitor simulator

    DEFF Research Database (Denmark)

    Donnarumma, I.; Evangelista, Y.; Campana, R.

    2012-01-01

    We present the simulator we developed for the Wide Field Monitor (WFM) aboard the Large Observatory For Xray Timing (LOFT) mission, one of the four ESA M3 candidate missions considered for launch in the 2022–2024 timeframe. The WFM is designed to cover a large FoV in the same bandpass as the Large...

  7. Large Observatory for x-ray Timing (LOFT-P): a Probe-class mission concept study

    Science.gov (United States)

    Wilson-Hodge, Colleen A.; Ray, Paul S.; Chakrabarty, Deepto; Feroci, Marco; Alvarez, Laura; Baysinger, Michael; Becker, Chris; Bozzo, Enrico; Brandt, Soren; Carson, Billy; Chapman, Jack; Dominguez, Alexandra; Fabisinski, Leo; Gangl, Bert; Garcia, Jay; Griffith, Christopher; Hernanz, Margarita; Hickman, Robert; Hopkins, Randall; Hui, Michelle; Ingram, Luster; Jenke, Peter; Korpela, Seppo; Maccarone, Tom; Michalska, Malgorzata; Pohl, Martin; Santangelo, Andrea; Schanne, Stephane; Schnell, Andrew; Stella, Luigi; van der Klis, Michiel; Watts, Anna; Winter, Berend; Zane, Silvia

    2016-07-01

    LOFT-P is a mission concept for a NASA Astrophysics Probe-Class (matter? What are the effects of strong gravity on matter spiraling into black holes? It would be optimized for sub-millisecond timing of bright Galactic X-ray sources including X-ray bursters, black hole binaries, and magnetars to study phenomena at the natural timescales of neutron star surfaces and black hole event horizons and to measure mass and spin of black holes. These measurements are synergistic to imaging and high-resolution spectroscopy instruments, addressing much smaller distance scales than are possible without very long baseline X-ray interferometry, and using complementary techniques to address the geometry and dynamics of emission regions. LOFT-P would have an effective area of >6 m2, > 10x that of the highly successful Rossi X-ray Timing Explorer (RXTE). A sky monitor (2-50 keV) acts as a trigger for pointed observations, providing high duty cycle, high time resolution monitoring of the X-ray sky with 20 times the sensitivity of the RXTE All-Sky Monitor, enabling multi-wavelength and multimessenger studies. A probe-class mission concept would employ lightweight collimator technology and large-area solid-state detectors, segmented into pixels or strips, technologies which have been recently greatly advanced during the ESA M3 Phase A study of LOFT. Given the large community interested in LOFT (>800 supporters*, the scientific productivity of this mission is expected to be very high, similar to or greater than RXTE ( 2000 refereed publications). We describe the results of a study, recently completed by the MSFC Advanced Concepts Office, that demonstrates that such a mission is feasible within a NASA probe-class mission budget.

  8. LOFT transient thermal analysis for 10 inch primary coolant blowdown piping weld

    International Nuclear Information System (INIS)

    Howell, S.K.

    1978-01-01

    A flaw in a weld in the 10 inch primary coolant blowdown piping was discovered by LOFT personnel. As a result of this, a thermal analysis and fracture mechanics analysis was requested by LOFT personnel. The weld and pipe section were analyzed for a complete thermal cycle, heatup and Loss of Coolant Experiment (LOCE), using COUPLE/MOD2, a two-dimensional finite element heat conduction code. The finite element representation used in this analysis was generated by the Applied Mechanics Branch. The record of nodal temperatures for the entire transient was written on tape VSN=T9N054, and has been forwarded to the Applied Mechanics Branch for use in their mechanical analysis. Specific details and assumptions used in this analysis are found in appropriate sections of this report

  9. The LOFT perspective on neutron star thermonuclear bursts: White paper in support of the mission concept of the large observatory for X-ray timing

    Energy Technology Data Exchange (ETDEWEB)

    in' t Zand, J. J.M. [SRON Netherlands Institute for Space Research, Utrecht (The Netherlands); Malone, Christopher M. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Altamirano, D. [Univ. of Southampton, Southampton (United Kingdom); Ballantyne, D. R. [Georgia Inst. of Technology, Atlanta, GA (United States); Bhattacharyya, S. [Tata Institute of Fundamental Research, Mumbai (India); Brown, E. F. [Michigan State Univ., East Lansing, MI (United States); Cavecchi, Y. [Univ. of Amsterdam, Amsterdam (The Netherlands); Chakrabarty, D. [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Chenevez, J. [Technical Univ. of Denmark, Lyngby (Denmark); Cumming, A. [McGill Univ., Montreal, QC (Canada); Degenaar, N. [Univ. of Cambridge, Cambridge (United Kingdom); Falanga, M. [International Space Science Institute, Bern (Switzerland); Galloway, D. K. [Monash Univ., VIC (Australia); Heger, A. [Monash Univ., VIC (Australia); Jose, J. [Univ. Politecnica de Catalunya, Barcelona (Spain); Institut d' Estudis Espacials de Catalunya, Barcelona (Spain); Keek, L. [Georgia Institute of Technology, Atlanta, GA (United States); Linares, M. [Univ. de La Laguna, Tenerife (Spain); Mahmoodifar, S. [Univ. of Maryland, College Park, MD (United States); Mendez, M. [Univ. of Groningen, Groningen (The Netherlands); Miller, M. C. [Univ. of Maryland, College Park, MD (United States); Paerels, F. B. S. [Columbia Astrophysics Lab., New York, NY (United States); Poutanen, J. [Univ. of Turku, Piikkio (Finland); Rozanska, A. [N. Copernicus Astronomical Center PAS, Warsaw (Poland); Schatz, H. [National Superconducting Cyclotron Laboratory at Michigan State University; Serino, M. [Institute of Physical and Chemical Research (RIKEN); Strohmayer, T. E. [NASA' s Goddard Space Flight Center, Greenbelt, MD (United States); Suleimanov, V. F. [Univ. Tubingen, Tubingen (Germany); Thielemann, F. -K. [Univ. Basel, Basel (Switzerland); Watts, A. L. [Univ. of Amsterdam, Amsterdam (The Netherlands); Weinberg, N. N. [Massachusetts Institute of Technology, Cambridge, MA (United States); Woosley, S. E. [Univ. of California, Santa Cruz, CA (United States); Yu, W. [Chinese Academy of Sciences (CAS), Shanghai (China); Zhang, S. [Institute of High-Energy Physics, Beijing (China); Zingale, M. [Stony Brook Univ., Stony Brook, NY (United States)

    2015-01-14

    The Large Area Detector (LAD) on the Large Observatory For X-ray Timing ( LOFT ), with a 8.5 m 2 photon- collecting area in the 2–30 keV bandpass at CCD-class spectral resolving power (λ/Δλ = 10 – 100), is designed for optimum performance on bright X-ray sources. Thus, it is well-suited to study thermonuclear X-ray bursts from Galactic neutron stars. These bursts will typically yield 2 x 105 photon detections per second in the LAD, which is at least 15 times more than with any other instrument past, current or anticipated. The Wide Field Monitor (WFM) foreseen for LOFT uniquely combines 2–50 keV imaging with large (30%) prompt sky coverage. This will enable the detection of tens of thousands of thermonuclear X-ray bursts during a 3-yr mission, including tens of superbursts. Both numbers are similar or more than the current database gathered in 50 years of X-ray astronomy.

  10. Assessment of RELAP/MOD2 using large break loss-of-coolant experimental data

    International Nuclear Information System (INIS)

    Kao, L.; Liao, L.Y.; Liang, K.S.; Wang, S.F.; Chen, Y.B.

    1989-01-01

    In this paper assessment of RELAP5/MOD2 using LOFT L2-5 and Semiscale S-06-3 tests are performed to provide information of the code capability and its limitation in analyzing large break LOCA of a nuclear power plant. Experiments L2-5 and S-06-3 are conducted to simulate a hypothetical LOCA which results from a 200% double-ended offset shear break in the cold-leg of a typical pressurized water reactor by utilizing scaling facilities of the LOFT and Semiscale Mod-1 systems, respectively. The RELAP5/MOD2 calculations for both tests begin with break initiation and subsequent blowdown, continue through lower plenum refill, core reflood, and terminate with corewide quench. Major phenomena of both large break loss-of-coolant tests are well predicted by RELAP5/MOD2. The results indicate that the break flow and system pressure are reasonably calculated. The cladding temperature response during blowdown period, which is the major importance to a large break LOCA, calculated by RELAP5/MOD2 shows good agreement with the test data

  11. Suggestion for a homogenizer installation in LOFT small break two-phase measurement

    International Nuclear Information System (INIS)

    Rieger, G.

    1981-07-01

    The purpose of this task, which was performed as an Austrian inkind contribution for the INEL research program is a) the evaluation of literature concerning homogenizers to improve two phase flow measurements for the LOFT small break test series, b) design of a homogenizer and c) recommandation of the location of a homogenizer in the LOFT piping system. To optimize the location of the homogenizer LTSF-tests should be performed according to the suggestions in this paper. (author)

  12. Investigation of effect of single phase electrical faults at LOFT

    International Nuclear Information System (INIS)

    Yeates, J.A.

    1978-01-01

    This LTR presents the general basic engineering facts related to an open phase fault in a three phase power system commonly referred to as a single phase condition. It describes the probable results to electrical motors and describes the LOFT system design factors which minimize the likelihood of such a fault occurring at LOFT. It recognizes that the hazard of such a fault is a realistic threat and notes the types of relays designed to provide protection. Recommendations are made to perform a detailed engineering study to determine the most advantageous protective relay design, and to implement such a design by installation of the necessary devices and controls

  13. LOFT experimental measurements uncertainty analyses. Volume XX. Fluid-velocity measurement using pulsed-neutron activation

    International Nuclear Information System (INIS)

    Lassahn, G.D.; Taylor, D.J.N.

    1982-08-01

    Analyses of uncertainty components inherent in pulsed-neutron-activation (PNA) measurements in general and the Loss-of-Fluid-Test (LOFT) system in particular are given. Due to the LOFT system's unique conditions, previously-used techniques were modified to make the volocity measurement. These methods render a useful, cost-effective measurement with an estimated uncertainty of 11% of reading

  14. The LOFT mission concept: a status update

    NARCIS (Netherlands)

    Feroci, M.; Bozzo, E.; Brandt, S.; Hernanz, M.; van der Klis, M.; Liu, L. -P; Orleanski, P.; Pohl, M.; Santangelo, A.; Schanne, S.; Stella, L.; Takahashi, T.; Tamura, H.; Watts, A.; Wilms, J.; Zane, S.; Zhang, S. -N; Bhattacharyya, S.; Agudo, I.; Ahangarianabhari, M.; Albertus, C.; Alford, M.; Alpar, A.; Altamirano, D.; Alvarez, L.; Amati, L.; Amoros, C.; Andersson, N.; Antonelli, A.; Argan, A.; Artigue, R.; Artigues, B.; Atteia, J. -L; Azzarello, P.; Bakala, P.; Ballantyne, D.; Baldazzi, G.; Baldo, M.; Balman, S.; Barbera, M.; van Baren, C.; Barret, D.; Baykal, A.; Begelman, M.; Behar, E.; Behar, O.; Belloni, T.; Bernardini, F.; Bertuccio, G.; Bianchi, S.; Bianchini, A.; Binko, P.; Blay, P.; Bocchino, F.; Bode, M.; Bodin, P.; Bombaci, I.; Bonnet Bidaud, J. -M; Boutloukos, S.; Bouyjou, F.; Bradley, L.; Braga, J.; Briggs, M. S.; Brown, E.; Buballa, M.; Bucciantini, N.; Burderi, L.; Burgay, M.; Bursa, M.; Budtz-Jørgensen, C.; Cackett, E.; Cadoux, F.; Cais, P.; Caliandro, G. A.; Campana, R.; Campana, S.; Cao, X.; Capitanio, F.; Casares, J.; Casella, P.; Castro-Tirado, A. J.; Cavazzuti, E.; Cavechi, Y.; Celestin, S.; Cerda-Duran, P.; Chakrabarty, D.; Chamel, N.; Château, F.; Chen, C.; Chen, Y.; Chenevez, J.; Chernyakova, M.; Coker, J.; Cole, R.; Collura, A.; Coriat, M.; Cornelisse, R.; Costamante, L.; Cros, A.; Cui, W.; Cumming, A.; Cusumano, G.; Czerny, B.; D'Aı, A.; D'Ammando, F.; D'Elia, V.; Dai, Z.; Del Monte, E.; De Luca, A.; De Martino, D.; Dercksen, J. P. C.; De Pasquale, M.; De Rosa, A.; Del Santo, M.; Di Cosimo, S.; Degenaar, N.; den Herder, J. W.; Diebold, S.; Di Salvo, T.; Dong, Y.; Donnarumma, I.; Doroshenko, V.; Doyle, G.; Drake, S. A.; Durant, M.; Emmanoulopoulos, D.; Enoto, T.; Erkut, M. H.; Esposito, P.; Evangelista, Y.; Fabian, A.; Falanga, M.; Favre, Y.; Feldman, C.; Fender, R.; Feng, H.; Ferrari, V.; Ferrigno, C.; Finger, M.; Finger, M. H.; Fraser, G. W.; Frericks, M.; Fullekrug, M.; Fuschino, F.; Gabler, M.; Galloway, D. K.; Gálvez Sanchez, J. L.; Gandhi, P.; Gao, Z.; Garcia-Berro, E.; Gendre, B.; Gevin, O.; Gezari, S.; Giles, A. B.; Gilfanov, M.; Giommi, P.; Giovannini, G.; Giroletti, M.; Gogus, E.; Goldwurm, A.; Goluchová, K.; Götz, D.; Gou, L.; Gouiffes, C.; Grandi, P.; Grassi, M.; Greiner, J.; Grinberg, V.; Groot, P.; Gschwender, M.; Gualtieri, L.; Guedel, M.; Guidorzi, C.; Guy, L.; Haas, D.; Haensel, P.; Hailey, M.; Hamuguchi, K.; Hansen, F.; Hartmann, D. H.; Haswell, C. A.; Hebeler, K.; Heger, A.; Hempel, M.; Hermsen, W.; Homan, J.; Hornstrup, A.; Hudec, R.; Huovelin, J.; Huppenkothen, D.; Inam, S. C.; Ingram, A.; In't Zand, J. J. M.; Israel, G.; Iwasawa, K.; Izzo, L.; Jacobs, H. M.; Jetter, F.; Johannsen, T.; Jenke, P. A.; Jonker, P.; Josè, J.; Kaaret, P.; Kalamkar, K.; Kalemci, E.; Kanbach, G.; Karas, V.; Karelin, D.; Kataria, D.; Keek, L.; Kennedy, T.; Klochkov, D.; Kluzniak, W.; Koerding, E.; Kokkotas, K.; Komossa, S.; Korpela, S.; Kouveliotou, C.; Kowalski, A. F.; Kreykenbohm, I.; Kuiper, L. M.; Kunneriath, D.; Kurkela, A.; Kuvvetli, I.; La Franca, F.; Labanti, C.; Lai, D.; Lamb, F. K.; Lachaud, C.; Laubert, P. P.; Lebrun, F.; Li, X.; Liang, E.; Limousin, O.; Lin, D.; Linares, M.; Linder, D.; Lodato, G.; Longo, F.; Lu, F.; Lund, N.; Maccarone, T. J.; Macera, D.; Maestre, S.; Mahmoodifar, S.; Maier, D.; Malcovati, P.; Malzac, J.; Malone, C.; Mandel, I.; Mangano, V.; Manousakis, A.; Marelli, M.; Margueron, J.; Marisaldi, M.; Markoff, S. B.; Markowitz, A.; Marinucci, A.; Martindale, A.; Martínez, G.; McHardy, I. M.; Medina-Tanco, G.; Mehdipour, M.; Melatos, A.; Mendez, M.; Mereghetti, S.; Migliari, S.; Mignani, R.; Michalska, M.; Mihara, T.; Miller, M. C.; Miller, J. M.; Mineo, T.; Miniutti, G.; Morsink, S.; Motch, C.; Motta, S.; Mouchet, M.; Mouret, G.; Mulačová, J.; Muleri, F.; Muñoz-Darias, T.; Negueruela, I.; Neilsen, J.; Neubert, T.; Norton, A. J.; Nowak, M.; Nucita, A.; O'Brien, P.; Oertel, M.; Olsen, P. E. H.; Orienti, M.; Orio, M.; Orlandini, M.; Osborne, J. P.; Osten, R.; Ozel, F.; Pacciani, L.; Paerels, F.; Paltani, S.; Paolillo, M.; Papadakis, I.; Papitto, A.; Paragi, Z.; Paredes, J. M.; Patruno, A.; Paul, B.; Pederiva, F.; Perinati, E.; Pellizzoni, A.; Penacchioni, A. V.; Peretz, U.; Perez, M. A.; Perez-Torres, M.; Peterson, B. M.; Petracek, V.; Pittori, C.; Pons, J.; Portell, J.; Possenti, A.; Postnov, K.; Poutanen, J.; Prakash, M.; Prandoni, I.; Le Provost, H.; Psaltis, D.; Pye, J.; Qu, J.; Rambaud, D.; Ramon, P.; Ramsay, G.; Rapisarda, M.; Rashevski, A.; Rashevskaya, I.; Ray, P. S.; Rea, N.; Reddy, S.; Reig, P.; Reina Aranda, M.; Remillard, R.; Reynolds, C.; Rezzolla, L.; Ribo, M.; de la Rie, R.; Riggio, A.; Rios, A.; Rischke, D. H.; Rodríguez-Gil, P.; Rodriguez, J.; Rohlfs, R.; Romano, P.; Rossi, E. M. R.; Rozanska, A.; Rousseau, A.; Rudak, B.; Russell, D. M.; Ryde, F.; Sabau-Graziati, L.; Sakamoto, T.; Sala, G.; Salvaterra, R.; Salvetti, D.; Sanna, A.; Sandberg, J.; Savolainen, T.; Scaringi, S.; Schaffner-Bielich, J.; Schatz, H.; Schee, J.; Schmid, C.; Serino, M.; Shakura, N.; Shore, S.; Schnittman, J. D.; Schneider, R.; Schwenk, A.; Schwope, A. D.; Sedrakian, A.; Seyler, J. -Y; Shearer, A.; Slowikowska, A.; Sims, M.; Smith, A.; Smith, D. M.; Smith, P. J.; Sobolewska, M.; Sochora, V.; Soffitta, P.; Soleri, P.; Song, L.; Spencer, A.; Stamerra, A.; Stappers, B.; Staubert, R.; Steiner, A. W.; Stergioulas, N.; Stevens, A. L.; Stratta, G.; Strohmayer, T. E.; Stuchlik, Z.; Suchy, S.; Suleimanov, V.; Tamburini, F.; Tauris, T.; Tavecchio, F.; Tenzer, C.; Thielemann, F. K.; Tiengo, A.; Tolos, L.; Tombesi, F.; Tomsick, J.; Torok, G.; Torrejon, J. M.; Torres, D. F.; Torresi, E.; Tramacere, A.; Traulsen, I.; Trois, A.; Turolla, R.; Turriziani, S.; Typel, S.; Uter, P.; Uttley, P.; Vacchi, A.; Varniere, P.; Vaughan, S.; Vercellone, S.; Vietri, M.; Vincent, F. H.; Vrba, V.; Walton, D.; Wang, J.; Wang, Z.; Watanabe, S.; Wawrzaszek, R.; Webb, N.; Weinberg, N.; Wende, H.; Wheatley, P.; Wijers, R.; Wijnands, R.; Wille, M.; Wilson-Hodge, C. A.; Winter, B.; Walk, S. J.; Wood, K.; Woosley, S. E.; Wu, X.; Xu, R.; Yu, W.; Yuan, F.; Yuan, W.; Yuan, Y.; Zampa, G.; Zampa, N.; Zampieri, L.; Zdunik, L.; Zdziarski, A.; Zech, A.; Zhang, B.; Zhang, C.; Zhang, S.; Zingale, M.; Zwart, F.

    2016-01-01

    The Large Observatory For x-ray Timing (LOFT) is a mission concept which was proposed to ESA as M3 and M4 candidate in the framework of the Cosmic Vision 2015-2025 program. Thanks to the unprecedented combination of effective area and spectral resolution of its main instrument and the uniquely large

  15. Statistical margin to DNB safety analysis approach for LOFT

    International Nuclear Information System (INIS)

    Atkinson, S.A.

    1982-01-01

    A method was developed and used for LOFT thermal safety analysis to estimate the statistical margin to DNB for the hot rod, and to base safety analysis on desired DNB probability limits. This method is an advanced approach using response surface analysis methods, a very efficient experimental design, and a 2nd-order response surface equation with a 2nd-order error propagation analysis to define the MDNBR probability density function. Calculations for limiting transients were used in the response surface analysis thereby including transient interactions and trip uncertainties in the MDNBR probability density

  16. Experiment data report for semiscale Mod-1 Test S-06-2 (LOFT counterpart test)

    International Nuclear Information System (INIS)

    Patton, M.L. Jr.; Collins, B.L.; Sackett, K.E.

    1977-08-01

    Recorded test data are presented for Test S-06-2 of the Semiscale Mod-1 LOFT counterpart test series. These tests are among several Semiscale Mod-1 experiments conducted to investigate the thermal and hydraulic phenomena accompanying an hypothesized loss-of-coolant accident in a pressurized water reactor (PWR) system. Test S-06-2 was conducted from initial conditions of 15 513 kPa and 563 K to investigate the response of the Semiscale Mod-1 system to a depressurization and reflood transient following a simulated double-ended offset shear of the broken loop cold leg piping. During the test, cooling water was injected into the cold leg of the intact loop to simulate emergency core coolant injection in a PWR. The heater rods in the electrically heated core were operated at an axial peak power density which was 50% of the maximum peak power density

  17. β-L-Arabinofuranosylation Conducted by 5-O-(2-pyridinecarbonyl)-L-arabinofuranosyl Trichloroacetimidate.

    Science.gov (United States)

    Li, Hong-Zhan; Ding, Jie; Cheng, Chun-Ru; Chen, Yue; Liang, Xing-Yong

    2018-05-02

    We describe a β-L-arabinofuranosylation method by employing the 5-O-(2-pyridinecarbonyl)-L-arabinofuranosyl trichloroacetimidate 10 as a donor. This approach allows a wide range of acceptor substrates, especially amino acid acceptors, to be used. Stereoselective synthesis of β-(1,4)-L-arabinofuranosyl-(2S, 4R)-4-hydroxy-L-proline (β-L-Araf-L-Hyp 4 ) and its dimer is achieved readily by this method. Both the stereoselectivities and yields of the reactions are excellent. To demonstrate the utility of this methodology, the preparation of a trisaccharide in a one-pot manner was carried out. Copyright © 2018 Elsevier Ltd. All rights reserved.

  18. Electrode Potentials of l-Tryptophan, l-Tyrosine, 3-Nitro-l-tyrosine, 2,3-Difluoro-l-tyrosine, and 2,3,5-Trifluoro-l-tyrosine.

    Science.gov (United States)

    Mahmoudi, Leila; Kissner, Reinhard; Nauser, Thomas; Koppenol, Willem H

    2016-05-24

    Electrode potentials for aromatic amino acid radical/amino acid couples were deduced from cyclic voltammograms and pulse radiolysis experiments. The amino acids investigated were l-tryptophan, l-tyrosine, N-acetyl-l-tyrosine methyl ester, N-acetyl-3-nitro-l-tyrosine ethyl ester, N-acetyl-2,3-difluoro-l-tyrosine methyl ester, and N-acetyl-2,3,5-trifluoro-l-tyrosine methyl ester. Conditional potentials were determined at pH 7.4 for all compounds listed; furthermore, Pourbaix diagrams for l-tryptophan, l-tyrosine, and N-acetyl-3-nitro-l-tyrosine ethyl ester were obtained. Electron transfer accompanied by proton transfer is reversible, as confirmed by detailed analysis of the current waves, and because the slopes of the Pourbaix diagrams obey Nernst's law. E°'(Trp(•),H(+)/TrpH) and E°'(TyrO(•),H(+)/TyrOH) at pH 7 are 0.99 ± 0.01 and 0.97 ± 0.01 V, respectively. Pulse radiolysis studies of two dipeptides that contain both amino acids indicate a difference in E°' of approximately 0.06 V. Thus, in small peptides, we recommend values of 1.00 and 0.96 V for E°'(Trp(•),H(+)/TrpH) and E°'(TyrO(•),H(+)/TyrOH), respectively. The electrode potential of N-acetyl-3-nitro-l-tyrosine ethyl ester is higher, while because of mesomeric stabilization of the radical, those of N-acetyl-2,3-difluoro-l-tyrosine methyl ester and N-acetyl-2,3,5-trifluoro-l-tyrosine methyl ester are lower than that of tyrosine. Given that the electrode potentials at pH 7 of E°'(Trp(•),H(+)/TrpH) and E°'(TyrO(•),H(+)/TyrOH) are nearly equal, they would be, in principle, interchangeable. Proton-coupled electron transfer pathways in proteins that use TrpH and TyrOH are thus nearly thermoneutral.

  19. A novel mechanism of RNase L inhibition: Theiler's virus L* protein prevents 2-5A from binding to RNase L

    Science.gov (United States)

    Drappier, Melissa; Elliott, Ruth; Zhang, Rong; Weiss, Susan R.; Silverman, Robert H.

    2018-01-01

    The OAS/RNase L pathway is one of the best-characterized effector pathways of the IFN antiviral response. It inhibits the replication of many viruses and ultimately promotes apoptosis of infected cells, contributing to the control of virus spread. However, viruses have evolved a range of escape strategies that act against different steps in the pathway. Here we unraveled a novel escape strategy involving Theiler’s murine encephalomyelitis virus (TMEV) L* protein. Previously we found that L* was the first viral protein binding directly RNase L. Our current data show that L* binds the ankyrin repeats R1 and R2 of RNase L and inhibits 2’-5’ oligoadenylates (2-5A) binding to RNase L. Thereby, L* prevents dimerization and oligomerization of RNase L in response to 2-5A. Using chimeric mouse hepatitis virus (MHV) expressing TMEV L*, we showed that L* efficiently inhibits RNase L in vivo. Interestingly, those data show that L* can functionally substitute for the MHV-encoded phosphodiesterase ns2, which acts upstream of L* in the OAS/RNase L pathway, by degrading 2-5A. PMID:29652922

  20. LOFT CIS analysis penetration S-11B 12'' H and V duct

    International Nuclear Information System (INIS)

    Condie, C.G.

    1978-01-01

    The 12 in. H and V Duct and related piping outside the LOFT containment and connected to containment penetration S-11B was analyzed to ASME Code, Subsection NC (Class 2) criteria. This duct is part of the Containment Isolation System. The model considered the duct from the containment O.D. outward through the second isolation valve. Results of this analysis show that this section of the line will meet Class 2 requirements without modification

  1. LOFT data acquisition and visual display system (DAVDS) presentation program

    International Nuclear Information System (INIS)

    Bullock, M.G.; Miyasaki, F.S.

    1976-03-01

    The Data Acquisition and Visual Display System (DAVDS) at the Loss-of-Fluid Test Facility (LOFT) has 742 data channel recording capability of which 576 are recorded digitally. The purpose of this computer program is to graphically present the data acquired and/or processed by the LOFT DAVDS. This program takes specially created plot data buffers of up to 1024 words and generates time history plots on the system electrostatic printer-plotter. The data can be extracted from two system input devices: Magnetic disk or digital magnetic tape. Versatility has been designed in the program by providing the user three methods of scaling plots: Automatic, control record, and manual. Time required to produce a plot on the system electrostatic printer-plotter varies from 30 to 90 seconds depending on the options selected. The basic computer and program details are described

  2. Lessons from cross-fleet/cross-airline observations - Evaluating the impact of CRM/LOFT training

    Science.gov (United States)

    Butler, Roy E.

    1991-01-01

    A review is presented of the crew resource management/line oriented flight training (CRM/LOFT) program to help determine the level of standardization across fleets and airlines in the critical area of evaluating crew behavior and performance. One of the goals of the project is to verify that check airmen and LOFT instructors within organizations are evaluating CRM issues consistently and that differences observed between fleets are not a function of idiosyncracies on the part of observers. Attention is given to the research tools for crew evaluation.

  3. MODIS/Aqua Clouds 5-Min L2 Swath 1km and 5km V006

    Data.gov (United States)

    National Aeronautics and Space Administration — The MODIS/Aqua Clouds 5-Min L2 Swath 1km and 5km (MYD06_L2) product consists of cloud optical and physical parameters. These parameters are derived using remotely...

  4. LOFT CIS analysis: 24'' H and V Duct outside penetration S-12A

    International Nuclear Information System (INIS)

    Pierce, A.T.

    1978-01-01

    The 24'' H and V Duct and related piping outside the LOFT containment and connected to containment penetration S-12A were analyzed to ASME Section III, Subsection NC (Class 2) criteria. The duct is part of the Containment Isolation System. The model considered the duct from the containment O.D. outward through the second isolation valve. Results of this analysis show that this section of the line will meet Class 2 requirements without modification

  5. Vibrational spectroscopic and theoretical study of 3,5-dimethyl-1-thiocarboxamide pyrazole (L) and the complexes Co2L2Cl4, Cu2L2Cl4 and Cu2L2Br2

    International Nuclear Information System (INIS)

    Nemcsok, Denes; Kovacs, Attila; Szecsenyi, Katalin Meszaros; Leovac, Vukadin M.

    2006-01-01

    In the present paper we report a joint experimental and theoretical study of 3,5-dimethyl-1-thiocarboxamide pyrazole (L) and its complexes Co 2 L 2 Cl 4 , Cu 2 L 2 Cl 4 and Cu 2 L 2 Br 2 . DFT computations were used to model the structural and bonding properties of the title compounds as well as to derive a reliable force field for the normal coordinate analysis of L. The computations indicated the importance of hydrogen bonding interactions in stabilising the global minimum structures on the potential energy surfaces. In contrast to the S-bridged binuclear Cu 2 L 2 Br 2 complex found in the crystal, our computations predicted the formation of (CuLBr) 2 dimers in the isolated state stabilized by very strong (53 kJ/mol) N-H...Br hydrogen bonding interactions. On the basis of FT-IR and FT-Raman experiments and the DFT-derived scaled quantum mechanical force field we carried out a complete normal coordinate analysis of L. The FT-IR spectra of the three complexes were interpreted using the present assignment of L, literature data and computed results

  6. Loft: An Automated Mesh Generator for Stiffened Shell Aerospace Vehicles

    Science.gov (United States)

    Eldred, Lloyd B.

    2011-01-01

    Loft is an automated mesh generation code that is designed for aerospace vehicle structures. From user input, Loft generates meshes for wings, noses, tanks, fuselage sections, thrust structures, and so on. As a mesh is generated, each element is assigned properties to mark the part of the vehicle with which it is associated. This property assignment is an extremely powerful feature that enables detailed analysis tasks, such as load application and structural sizing. This report is presented in two parts. The first part is an overview of the code and its applications. The modeling approach that was used to create the finite element meshes is described. Several applications of the code are demonstrated, including a Next Generation Launch Technology (NGLT) wing-sizing study, a lunar lander stage study, a launch vehicle shroud shape study, and a two-stage-to-orbit (TSTO) orbiter. Part two of the report is the program user manual. The manual includes in-depth tutorials and a complete command reference.

  7. Low-order model of the Loss-of-Fluid Test (LOFT) reactor plant for use in Kalman filter-based optimal estimators

    International Nuclear Information System (INIS)

    Tylee, J.L.

    1980-01-01

    A low-order, nonlinear model of the Loss-of-Fluid Test (LOFT) reactor plant, for use in Kalman filter estimators, is developed, described, and evaluated. This model consists of 31 differential equations and represents all major subsystems of both the primary and secondary sides of the LOFT plant. Comparisons between model calculations and available LOFT power range testing transients demonstrate the accuracy of the low-order model. The nonlinear model is numerically linearized for future implementation in Kalman filter and optimal control algorithms. The linearized model is shown to be an adequate representation of the nonlinear plant dynamics

  8. LOFT CIS analysis penetration S-7A 8'' WW - 172-AB

    International Nuclear Information System (INIS)

    Barry, W.J. Jr.

    1978-01-01

    The 8'' WW - 172 - AB line from the containment penetration S-7A was analyzed to ASME Code, Subsection NC (Class 2) criteria. This section of piping is part of the LOFT Containment Isolation System. The model considered the line from the penetration S-7A outward through two horizontal-plane elbows and then through three valves to a wall penetration. Results of the analysis show that the system will meet Class 2 requirements if additional support/restraint is installed at three locations besides the existing restraints. The locations and loads for these supports/restraints are included in the body of the report

  9. Delayed vertebral diagnosed L4 pincer vertebral fracture, L2-L3 ruptured vertebral lumbar disc hernia, L5 vertebral wedge fracture - Case report

    Directory of Open Access Journals (Sweden)

    Balasa D

    2016-08-01

    Full Text Available An association between delayed ruptured lumbar disc hernia, L5 vertebral wedge fracture and posttraumaticL4 pincer vertebral fracture (A2.3-AO clasification at different levels is a very rare entity. We present the case of a 55 years old male who falled down from a bicycle. 2 months later because of intense and permanent vertebral lumbar and radicular L2 and L3 pain (Visual Scal Autologus of Pain7-8/10 the patient came to the hospital. He was diagnosed with pincer vertebral L4 fracture (A2.3-AO clasification and L2-L3 right ruptured lumbar disc hernia in lateral reces. The patient was operated (L2-L3 right fenestration, and resection of lumbar disc hernia, bilateral stabilisation, L3-L4-L5 with titan screws and postero-lateral bone graft L4 bilateral harvested from iliac crest.

  10. TRAC-PF1 MOD1 post test calculations of the OECD LOFT Experiment LP-SB-1

    International Nuclear Information System (INIS)

    Allen, E.J.

    1990-04-01

    Analysis of the small, hot leg break, OECD LOFT Experiment LP-SB-1. using the ''best-estimate'' computer code TRAC-PF1/MOD1 is presented. Descriptions of the LOFT facility and the LP-SB-1 experiment are given and development of the TRAC-PF1/MOD1 input model is detailed. The calculations performed in achieving the steady state conditions, from which the experiment was initiated, and the specification of experimental boundary conditions are outlined. 24 refs., 66 figs., 12 tabs

  11. MODIS/Aqua Temperature and Water Vapor Profiles 5-Min L2 Swath 5km V006

    Data.gov (United States)

    National Aeronautics and Space Administration — MODIS/Aqua Temperature and Water Vapor Profiles 5-Min L2 Swath 5km (MYD07_L2). MODIS was launched aboard the Aqua satellite on May 04, 2002 (1:30 pm equator crossing...

  12. Crystal and molecular structure of the coordination compounds of Er3+ with 1-(methoxydiphenylphosphoryl)-2-diphenylphosphorylbenzene [ErL21(NO3)2]2[Er(NO3)2(H2O)5]0.333(NO3)2.333 · 2.833H2O and its ethyl substituted derivative [ErL22(NO3)2][Er(NO3)5]0.5 · 0.5H2O

    International Nuclear Information System (INIS)

    Polyakova, I. N.; Baulin, V. E.; Ivanova, I. S.; Pyatova, E. N.; Sergienko, V. S.; Tsivadze, A. Yu.

    2015-01-01

    The coordination compounds of Er 3+ with 1-(methoxydiphenylphosphoryl)-2-diphenylphosphorylbenzene [ErL 2 1 (NO 3 ) 2 ] 2 [Er(NO 3 ) 2 (H 2 O) 5 ] 0.333 (NO 3 ) 2.333 · 2.833H 2 O (I) and its ethyl substituted derivative [ErL 2 2 (NO 3 ) 2 ][Er(NO 3 ) 5 ] 0.5 · 0.5H 2 O (II) are synthesized and their crystal structures are studied. I and II contain [ErL 2 (NO 3 ) 2 ] + complex cations of identical composition and close structure. The eight-vertex polyhedron of the Er atom in the shape of a distorted octahedron with two split trans vertices is formed by the O atoms of the phosphoryl groups of L ligands and nitrate anions. L ligands close nine-membered metallocycles. The structures contain spacious channels which are populated differently, namely, by disordered [Er(NO 3 ) 2 (H 2 O) 5 ] + complex cations, NO 3 − anions, and crystallization water molecules in I and disordered [Er(NO 3 ) 5 ] 2− complex anions and crystallization water molecules in II. The IR spectra of I and II are studied

  13. The LOFT Burst Alert System and its Burst On-board Trigger

    DEFF Research Database (Denmark)

    Schanne, Stephane; Götz, Diego; Provost, Herve Le

    2014-01-01

    The ESA M3 candidate mission LOFT (Large Observatory For x-ray Timing) has been designed to study strong gravitational fields by observing compact objects, such as black-hole binaries or neutron-star systems and supermassive black-holes, based on the temporal analysis of photons collected...

  14. Stress analysis of LOFT steam generator blowdown cross-over line

    International Nuclear Information System (INIS)

    Singh, J.N.

    1978-01-01

    The purpose of this report is to demonstrate compliance of the LOFT Steam Generator Blowdown Cross-Over Piping with the ASME Boiler and Pressure Vessel Code, Section III, Subsection NC. Deadweight, thermal expansion, seismic, LOCE, and LOCA loads have been considered. With the addition of two snubbers, as shown in this report, the system conforms to all requirements

  15. Seismic stress analysis of feeder lines to LOFT primary coolant pump motors

    International Nuclear Information System (INIS)

    Kuehster, C.J.

    1978-01-01

    The conduit system in the LOFT Support Building was analyzed for seismic loading. The conduit itself plus its various supports were subjected to both horizontal and vertical forces. The results show the system loads or stresses to be within allowables

  16. Requalification of the LOFT reactor following a loss of coolant experiment (Level I)

    International Nuclear Information System (INIS)

    Cannon, J.W.

    1979-01-01

    During a Loss of Coolant Experiment (LOCE), the LOFT reactor experiences an acceleration of 10 G's and fuel cladding temperature changes at a rate of 1100 0 K/sec. These unparalleled conditions present a unique startup problem to the LOFT program: How can the integrity of the fuel be confirmed so as to minimize operation if damage has occurred. The Level I Requalification Program is designed to accomplish this. It is a progressive series of tests, designed to detect damage at the earliest possible time, and thus preclude or minimize operation if damage exists. First, fuel specialists examine the LOCE data for possible damaging conditions and the results of primary coolant sample analysis for signs of failed fuel. Second, the requalification program proceeds to a series of mechanical and physics tests

  17. Association of Immune and Metabolic Receptors C5aR and C5L2 with Adiposity in Women

    Science.gov (United States)

    Rezvani, Reza; Gupta, Abhishek; Marceau, Picard; Tchernof, André

    2014-01-01

    Adipose tissue receptors C5aR and C5L2 and their heterodimerization/functionality and interaction with ligands C5a and acylation stimulating protein (ASP) have been evaluated in cell and rodent studies. Their contribution to obesity factors in humans remains unclear. We hypothesized that C5a receptors, classically required for host defense, are also associated with adiposity. Anthropometry and fasting blood parameters were measured in 136 women divided by body mass index (BMI): normal/overweight (≤30 kg/m2; n = 34), obese I (≤45 kg/m2; n = 33), obese II (≤51 kg/m2; n = 33), and obese III (≤80 kg/m2; n = 36). Subcutaneous and omental adipose tissue C5aR and C5L2 expression were analysed. C5L2 expression was comparable between subcutaneous and omental across all BMI groups. Plasma ASP and ASP/omental C5L2 expression increased with BMI (P correlations between C5L2/C5aR and waist circumference, HDL-C, and adiponectin. Tissue and BMI differences in receptors and ligands, particularly in omental, suggest relationship to metabolic disturbances and highlight adipose-immune interactions. PMID:24523571

  18. LOFT CIS analysis: 24'' H and V Duct outside penetration S-12A

    Energy Technology Data Exchange (ETDEWEB)

    Pierce, A.T.

    1978-08-18

    The 24'' H and V Duct and related piping outside the LOFT containment and connected to containment penetration S-12A were analyzed to ASME Section III, Subsection NC (Class 2) criteria. The duct is part of the Containment Isolation System. The model considered the duct from the containment O.D. outward through the second isolation valve. Results of this analysis show that this section of the line will meet Class 2 requirements without modification.

  19. LOFT advanced fuel rod instrumentation development

    International Nuclear Information System (INIS)

    Billeter, T.R.; Brown, R.L.; Chan, A.I.Y.; Day, C.K.; Meyers, S.C.; Sheen, E.M.; Stringer, J.L.

    1978-01-01

    Advanced fuel rod instrumentation for the Loss of Fluid Test (LOFT) reactor is being developed by the Hanford Engineering Development Laboratory for the Nuclear Regulatory Commission. This effort calls for development of sensors to measure fuel rod axial motion, fuel centerline temperature (to 2200 0 C), fuel rod plenum gas pressure (to 2500 psig), and plenum gas temperature (to 1500 0 F). A parallel test and evaluation of several modified commercial sensors was undertaken and will result in commercial availability of the final qualified sensors. Necessary test facilities were prepared for the development and evaluation effort. Tests to date indicate a three coil Linear Variable Differential Transformer (LVDT), operated from temperature compensating signal source and processing electronics, will meet the desired requirements

  20. Probing the emission physics and weak/soft population of Gamma-Ray Bursts with LOFT. White Paper in Support of the Mission Concept of the Large Observatory for X-ray Timing

    DEFF Research Database (Denmark)

    Amati, L.; Stratta, G.; Atteia, J.L.

    of ultra- dense matter in neutron stars? Does matter orbiting close to the event horizon follow the predictions of general relativity? These goals are elaborated in the mission Yellow Book ( http://sci.esa.int/loft/ 53447-loft-yellow-book/ ) describing the LOFT mission as proposed in M3, which closely...

  1. Delayed vertebral diagnosed L4 pincer vertebral fracture, L2-L3 ruptured vertebral lumbar disc hernia, L5 vertebral wedge fracture - Case report

    OpenAIRE

    Balasa D; Schiopu M; Tunas A; Baz R; Hancu Anca

    2016-01-01

    An association between delayed ruptured lumbar disc hernia, L5 vertebral wedge fracture and posttraumaticL4 pincer vertebral fracture (A2.3-AO clasification) at different levels is a very rare entity. We present the case of a 55 years old male who falled down from a bicycle. 2 months later because of intense and permanent vertebral lumbar and radicular L2 and L3 pain (Visual Scal Autologus of Pain7-8/10) the patient came to the hospital. He was diagnosed with pincer vertebral L4 fracture (A2....

  2. Status of the assessment phase of the ESA M3 mission candidate LOFT

    Science.gov (United States)

    Corral van Damme, Carlos; Ayre, Mark; Lumb, David; Short, Alexander D.; Rando, Nicola

    2012-09-01

    LOFT (Large Observatory For x-ray Timing) is one of four candidates for the M3 slot (launch in 2024, with the option of a launch in 2022) of ESAs Cosmic Vision 2015 - 2025 Plan, and as such it is currently undergoing an initial assessment phase lasting one year. The objective of the assessment phase is to provide the information required to enable the down selection process, in particular: the space segment definition for meeting the assigned science objectives; consideration of and initial definition of the implementation schedule; an estimate of the mission Cost at Completion (CaC); an evaluation of the technology readiness evaluation and risk assessment. The assessment phase is divided into two interleaved components: (i) A payload assessment study, performed by teams funded by member states, which is primarily intended for design, definition and programmatic/cost evaluation of the payload, and (ii) A system industrial study, which has essentially the same objectives for the space segment of the mission. This paper provides an overview of the status of the LOFT assessment phase, both for payload and platform. The initial focus is on the payload design status, providing the reader with an understanding of the main features of the design. Then the space segment assessment study status is presented, with an overview of the principal challenges presented by the LOFT payload and mission requirements, and a presentation of the expected solutions. Overall the mission is expected to enable cutting-edge science, is technically feasible, and should remain within the required CaC for an M3 candidate.

  3. Deficiency of C5L2 increases macrophage infiltration and alters adipose tissue function in mice.

    Directory of Open Access Journals (Sweden)

    Danny Gauvreau

    Full Text Available BACKGROUND: Obesity is considered as a systemic chronic low grade inflammation characterized by increased serum pro-inflammatory proteins and accumulation of macrophages within white adipose tissue (WAT of obese patients. C5L2, a 7-transmembrane receptor, serves a dual function, binding the lipogenic hormone acylation stimulating protein (ASP, and C5a, involved in innate immunity. AIM: We evaluated the impact of C5L2 on macrophage infiltration in WAT of wildtype (Ctl and C5L2 knock-out (C5L2(-/- mice over 6, 12 and 24 weeks on a chow diet and moderate diet-induced obesity (DIO conditions. RESULTS: In Ctl mice, WAT C5L2 and C5a receptor mRNA increased (up to 10-fold both over time and with DIO. By contrast, in C5L2(-/-, there was no change in C5aR in WAT. C5L2(-/- mice displayed higher macrophage content in WAT, varying by time, fat depot and diet, associated with altered systemic and WAT cytokine patterns compared to Ctl mice. However, in all cases, the M1 (pro- vs M2 (anti-inflammatory macrophage proportion was unchanged but C5L2(-/- adipose tissue secretome appeared to be more chemoattractant. Moreover, C5L2(-/- mice have increased food intake, increased WAT, and altered WAT lipid gene expression, which is reflected systemically. Furthermore, C5L2(-/- mice have altered glucose/insulin metabolism, adiponectin and insulin signalling gene expression in WAT, which could contribute to development of insulin resistance. CONCLUSION: Disruption of C5L2 increases macrophage presence in WAT, contributing to obesity-associated pathologies, and further supports a dual role of complement in WAT. Understanding this effect of the complement system pathway could contribute to targeting treatment of obesity and its comorbidities.

  4. Sediment Lofting From Melt-Water Generated Turbidity Currents During Heinrich Events as a Tool to Assess Main Sediment Delivery Phases to Small Subpolar Ocean Basins

    Science.gov (United States)

    Hesse, R.

    2009-05-01

    Small subpolar ocean basins such as the Labrador Sea received a major portion (25%) of their sediment fill during the Pleistocene glaciations (less than 5% of the basin's lifetime), but the detailed timing of sediment supply to the basin remained essentially unknown until recently. The main sediment input into the basin was probably not coupled to major glacial cycles and associated sea-level changes but was related to Heinrich events. Discovery of the depositional facies of fine-grained lofted sediment provides a tool which suggests that the parent-currents from which lofting took place may have been sandy-gravelly turbidity currents that built a huge braided abyssal plain in the Labrador Sea (700 by 120 km underlain by 150 m on average of coarse- grained sediment) which is one of the largest sand accumulations (104 km3) on Earth. The facies of lofted sediment consists of stacked layers of graded muds that contain ice-rafted debris (IRD) which impart a bimodal grain-size distribution to the graded muds. The texturally incompatible grain populations of the muds (median size between 4 and 8 micrometers) and the randomly distributed coarse silt and sand-sized IRD require the combination of two transport processes that delivered the populations independently and allowed mixing at the depositional site: (i) sediment rafting by icebergs (dropstones) and (ii) the rise of turbid freshwater plumes out of fresh-water generated turbidity currents. Sediment lofting from turbidity currents is a process that occurs in density currents generated from sediment-laden fresh-water discharges into the sea that can produce reversed buoyancy, as is well known from experiments. When the flows have traveled long enough, their tops will have lost enough sediment by settling so that they become hypopycnal (their density decreasing below that of the ambient seawater) causing the current tops to lift up. The turbid fresh-water clouds buoyantly rise out of the turbidity current to a level of

  5. LOFT fuel modules design, characterization, and fabrication program

    International Nuclear Information System (INIS)

    Russell, M.L.

    1977-06-01

    The loss-of-fluid test [LOFT) fuel modules have evolved from a comprehensive five-year design, characterization, and fabrication program which has resulted in the accomplishment of many technical activities of interest in pressurized water reactor fuel design development and safety research. Information is presented concerning: determination of fundamental high-temperature reactor material properties; design invention related to in-core instrumentation attachment; implementation of advanced and/or unique fuel bundle characterization techniques; implementation of improved fuel bundle fabrication techniques; and planning and execution of a multimillion dollar design, characterization, and fabrication program for pressurized water reactor fuel

  6. MODIS/Aqua Aerosol 5-Min L2 Swath 10km V006

    Data.gov (United States)

    National Aeronautics and Space Administration — The MODIS/Aqua Aerosol 5-Min L2 Swath 10km (MYD04_L2) product continues to provide full global coverage of aerosol properties from the Dark Target (DT) and Deep Blue...

  7. Loft CIS analysis 2''-LS-118-AB outside containment penetration S5-D

    International Nuclear Information System (INIS)

    Morton, D.K.

    1978-01-01

    A stress analysis was performed on the 2''-LS-118-AB pipe system outside containment penetration S5-D. Deadweight, thermal expansion, and seismic loads were considered. The results indicate that this piping will meet ASME Section III, Class 2 requirements provided a U-bolt (S4) is installed as indicated in this report

  8. Pseudolisthesis secondary to small L5

    International Nuclear Information System (INIS)

    Bagheri, Mohammad Hadi; Hosseini, Mehrdad Mohammad; Foroughi, Amin Aboulhassani

    2011-01-01

    Rationale and objective: Small AP diameter of L5 can mimic spondylolisthesis in lumbosacral MRI. The aim of the present study was to evaluate the prevalence of small L5 mimicking spondylolisthesis in lumbosacral MRI of the patients referred to an imaging center by any symptoms. Association between small anteroposterior diameter of L5 and presence and absence of unilateral or bilateral spondylolysis at L5 were also evaluated. Materials and methods: 2000 lumbosacral MRI of the patients referred to an imaging center for any reasons were evaluated. The posterior step of L5 was measured and the cases with posterior step of more than 2 mm were selected. These cases were evaluated for presence or absence of spondylolysis. Results: Small anteroposterior diameters of L5 were detected in 38 cases (2%), mimicking the spondylolisthesis in lumbosacral MRI. The prevalence of small L5 in lumbosacral MRI of the patients referred by any symptoms was about 2%. In these selected cases, 6 (15.8%) show unilateral spondylolysis and 5 (13.1%) show bilateral spondylolysis. Conclusion: Pseudospondylolisthesis secondary to small AP diameter of L5 has prevalence of about 2% in lumbosacral MRI of cases referred for any reasons and can be overlooked in reporting of LS MRI. There was an association between small anteroposterior diameter of L5 and presence of spondylolysis at L5.

  9. LOFT blowdown loop piping thermal analysis Class I review

    International Nuclear Information System (INIS)

    Kinnaman, T.L.

    1978-01-01

    In accordance with ASME Code, Section III requirements, all analyses of Class I components must be independently reviewed. Since the LOFT blowdown loop piping up through the blowdown valve is a Class I piping system, the thermal analyses are reviewed. The Thermal Analysis Branch comments to this review are also included. It is the opinion of the Thermal Analysis Branch that these comments satisfy all of the reviewers questions and that the analyses should stand as is, without additional considerations in meeting the ASME Code requirements and ANC Specification 60139

  10. 5-Acetamido-3,5-dideoxy-L-glycero-L-manno-non-2-ulosonic acid-containing O-polysaccharide from marine bacterium Pseudomonas glareae KMM 9500T.

    Science.gov (United States)

    Kokoulin, Maxim S; Kalinovsky, Anatoly I; Romanenko, Lyudmila A; Mikhailov, Valery V

    2018-05-22

    The O-polysaccharide was isolated from the lipopolysaccharide of a marine bacterium Pseudomonas glareae KMM 9500 T and studied by chemical methods along with 1D and 2D 1 H and 13 C NMR spectroscopy including 1 H, 1 H-TOCSY, 1 H, 1 H-COSY, 1 H, 1 H-ROESY, 1 H, 13 C-HSQC and 1 H, 13 C-HMBC experiments. The O-polysaccharide was found to consist of linear tetrasaccharide repeating units constituted by D-glucuronic acid (D-GlcA), L-rhamnose (L-Rha), D-glucose (D-Glc) and 5-acetamido-7,9-O-[(S)-1-carboxyethylidene]-3,5-dideoxy-L-glycero-L-manno-non-2-ulosonic acid (Sug7,9(S-Pyr)), partially O-acetylated at position 8 (∼70%): →4)-α-D-GlcpA-(1→3)-β-L-Rhap-(1→4)-β-D-Glcp-(1→4)-β-Sugp8Ac(∼70%)7,9(S-Pyr)-(2→. Copyright © 2018 Elsevier Ltd. All rights reserved.

  11. Background simulations for the Large Area Detector onboard LOFT

    DEFF Research Database (Denmark)

    Campana, Riccardo; Feroci, Marco; Ettore, Del Monte

    2013-01-01

    and magnetic fields around compact objects and in supranuclear density conditions. Having an effective area of similar to 10 m(2) at 8 keV, LOFT will be able to measure with high sensitivity very fast variability in the X-ray fluxes and spectra. A good knowledge of the in-orbit background environment...... is essential to assess the scientific performance of the mission and optimize the design of its main instrument, the Large Area Detector (LAD). In this paper the results of an extensive Geant-4 simulation of the instrumentwillbe discussed, showing the main contributions to the background and the design...... an anticipated modulation of the background rate as small as 10 % over the orbital timescale. The intrinsic photonic origin of the largest background component also allows for an efficient modelling, supported by an in-flight active monitoring, allowing to predict systematic residuals significantly better than...

  12. LOFT liquid level transducer application techniques and measurement uncertainty

    International Nuclear Information System (INIS)

    Batt, D.L.; Biladeau, G.L.; Goodrich, L.D.; Nightingale, C.M.

    1979-01-01

    A conductivity sensitive liquid level transducer (LLT) has been designed and used successfully for determining whether steam or water is present in the Loss-of-Fluid Tests (LOFT) performed by EG and G Idaho, Inc., at the Idaho National Engineering Laboratory. The presence of steam or water is determined by establishing a discriminator level which is set manually. A computer program establishes the presence or absence of water for each data point taken. In addition to liquid level, the LLT is used for reactor vessel mass and volume calculations. The uncertainty in the liquid level is essentially the spacing of the LLT electrodes

  13. Pressure sensor for use in the Loss-of-Fluid-Test (LOFT) reactor

    International Nuclear Information System (INIS)

    Billeter, T.R.

    1979-07-01

    Tests at temperatures up to 800 0 F and pressures up to 2500 psig were conducted at Hanford Engineering Development Laboratory (HEDL) to qualify an instrument for measurement of fuel-rod pressure in the Loss-of-Fluid-Test (LOFT) reactor. Operational characteristics of the selected pressure transducers are summarized for a series of static, quasi-static, and transient tests conducted for a period of about 700 hours

  14. Fracture mechanics evaluation of LOFT lower plenum injection nozzle

    International Nuclear Information System (INIS)

    Nagata, P.K.; Reuter, W.G.

    1977-01-01

    An analysis to establish whether or not a leak-before-break concept would apply to the LOFT lower plenum injection nozzle is described. The analysis encompassed the structure from the inlet side of valve V-2170 to the lower plenum nozzle-to-reactor vessel weld on the left side of the emergency core cooling system (ECCS). The defect that was assumed to exist was of such a size that the probability of its being missed by the applicable inspection technique was near zero. The Inconel 600 nozzle forging with an initial assumed defect size of 0.64 cm (0.25 in.) deep would behave as follows: (1) the axially oriented defect would result in leak before rupture (the number of cycles to rupture was 11,000), (2) the circumferentially oriented defect would result in a rupture before leak. The number of cycles to failure would be in excess of 14,000. Based on the conservative assumption that the thermal stresses were membrane stresses as opposed to a bending stress, the following were found. For the Inconel 82 weld metal (thickness of 1.3 cm [0.53 in.]) and AISI 316 SST valve body, with an initial assumed defect of 0.25 cm (0.1 in.), the crack would grow through the thickness in a minimum of 3950 cycles and to a critical rupture crack length of 5.1 cm (2.0 in.) in an additional 80 cycles. The Inconel 82 weld metal at the shell body (thickness of 9.7 cm or 3.8 in.) with an assumed defect 1.3 cm (0.5 in.) deep would fail in 334 cycles. Calculations made assuming a linear stress gradient instead of the above-mentioned flat distribution through the wall indicated that the number of stress cycles increased to 2200

  15. Results and analysis of a loss-of-feedwater induced ATWS experiment in the LOFT Facility

    International Nuclear Information System (INIS)

    Grush, W.H.; Koizumi, Y.; Woerth, S.C.

    1983-01-01

    An anticipated transient without scram (ATWS), initiated by a loss of feedwater, was experimentally simulated in the Loss-of-Fluid Test (LOFT) pressurized water reactor (PWR). Primary system pressure was controlled using a two-position actuator relief valve to simulate a scaled power-operated relief valve (PORV) and safety relief valve (SRV) representative of those in a commercial PWR. Auxiliary feedwater injection was delayed during the experiment until the plant recovery phase where long-term shutdown was achieved by an operator-controlled plant recovery procedure without inserting the control rods. The system transient response predicted by the RELAP5/MOD1 computer code showed good agreement with the experimental data

  16. Capability of LOFT vital batteries to supply emergency power demands during severe cold weather conditions

    International Nuclear Information System (INIS)

    Yeates, J.A.

    1978-01-01

    This study evaluates the capability of the vital batteries (PPS) to provide electrical power via the vital DC-AC motor generator sets to the LOFT PPS loads during severe cold weather conditions. It is concluded that these batteries while at a temperature of 5 0 F will supply the necessary PPS electrical loads for a time in excess of the one hour permitted to start the diesel generators and are, therefore, adequate at this temperature. This Revision B of the LTR includes revised, more recent, and complete technical data relating to MG set efficiency, battery operating procedures and cold temperature derating. Revision B supersedes and replaces all previous issues

  17. Measurement properties of the EQ-5D-5L compared to the EQ-5D-3L across eight patient groups

    DEFF Research Database (Denmark)

    Janssen, M F; Pickard, A Simon; Golicki, Dominik

    2012-01-01

    PURPOSE: The aim of this study was to assess the measurement properties of the 5-level classification system of the EQ-5D (5L), in comparison with the 3-level EQ-5D (3L). METHODS: Participants (n = 3,919) from six countries, including eight patient groups with chronic conditions (cardiovascular...... of the 3-level system which improves upon the measurement properties, reducing the ceiling while improving discriminatory power and establishing convergent and known-groups validity.......), redistribution properties, ceiling, discriminatory power, convergent validity, and known-groups validity. RESULTS: Missing values were on average 0.8 % for 5L and 1.3 % for 3L. In total, 2.9 % of responses were inconsistent between 5L and 3L. Redistribution from 3L to 5L using EQ dimension-specific rating scales...

  18. Cross-cultural measurement equivalence of the 5-level EQ-5D (EQ-5D-5L) in patients with type 2 diabetes mellitus in Singapore.

    Science.gov (United States)

    Wang, Ye; Tan, Ngiap-Chuan; Tay, Ee-Guan; Thumboo, Julian; Luo, Nan

    2015-07-16

    This study aimed to assess the measurement equivalence of the 5-level EQ-5D (EQ-5D-5L) among the English, Chinese, and Malay versions. A convenience sample of patients with type 2 diabetes mellitus were enrolled from a public primary health care institution in Singapore. The survey questionnaire comprised the EQ-5D-5L and questions assessing participants' socio-demographic and clinical characteristics. Multiple linear regression models were used to assess the difference in EQ-5D-5L index (calculated using an interim algorithm) and EQ-visual analog scale (EQ-VAS) scores across survey language (Chinese vs. English, Malay vs. English, and Malay vs. Chinese). Measurement equivalence was examined by comparing the 90% confidence interval of difference in the EQ-5D-5L index and EQ-VAS scores with a pre-determined equivalence margin. Multiple logistic regression models were used to assess the response patterns of the 5 Likert-type items of the EQ-5D-5L across survey language. Equivalence was demonstrated between the Chinese and English versions and between the Malay and English versions of the EQ-5D-5L index scores. Equivalence was also demonstrated between the Chinese and English versions and between the Malay and Chinese versions of the EQ-VAS scores. Equivalence could not be determined between the Malay and Chinese versions of the EQ-5D-5L index score and between the Malay and English versions of the EQ-VAS score. No significant difference was found in responses to EQ-5D-5L items between any languages, except that patients who chose to complete the Chinese version were more likely to report "no problems" in mobility compared to those who completed the Malay version of the questionnaire. This study provided evidence for the measurement equivalence of the different language versions of EQ-5D-5L in Singapore.

  19. A qualitative examination of the content validity of the EQ-5D-5L in patients with type 2 diabetes.

    Science.gov (United States)

    Matza, Louis S; Boye, Kristina S; Stewart, Katie D; Curtis, Bradley H; Reaney, Matthew; Landrian, Amanda S

    2015-12-01

    The EQ-5D is frequently used to derive utilities for patients with type 2 diabetes (T2D). Despite widely available quantitative psychometric data on the EQ-5D, little is known about content validity in this population. Thus, the purpose of this qualitative study was to examine content validity of the EQ-5D in patients with T2D. Patients with T2D in the UK completed concept elicitation interviews, followed by administration of the EQ-5D-5L and cognitive interviewing focused on the instrument's relevance, clarity, and comprehensiveness. A total of 25 participants completed interviews (52.0 % male; mean age = 53.5 years). Approximately half (52 %) reported that the EQ-5D-5L was relevant to their experience with T2D. When asked if each individual item was relevant to their experience with T2D, responses varied widely (24.0 % said the self-care item was relevant; 68.0 % said the anxiety/depression item was relevant). Participants frequently said items were not relevant to themselves, but could be relevant to patients with more severe diabetes. Most participants (92.0 %) reported that T2D and/or its treatment/monitoring requirements had an impact on their quality of life that was not captured by the EQ-5D-5L. Common missing concepts included food awareness/restriction (n = 13, 52.0 %); activities (n = 11, 44.0 %); emotional functioning other than depression/anxiety (n = 8, 32.0 %); and social/relationship functioning (n = 8, 32.0 %). The results highlight strengths and potential limitations of the EQ-5D-5L, including missing content that could be important for some patients with T2D. Suggestions for addressing limitations are provided.

  20. TRAC-PF1 code assessment using OECD LOFT LP-FP-1 experiment

    International Nuclear Information System (INIS)

    Barbero, F.J.

    1992-04-01

    This report assesses thermal-hydraulic aspects of LOFT LP-FP-1 experiment making use of TRAC-PF1/MOD1. LP-FP-1 experiment studies the system thermal-hydraulic and core thermal response for initial and boundary conditions similar to a large-break design basis LOCA leading to fission product release from the fuel cladding gap region. It also assesses the fission product retention effectiveness of the PWR-ECCS in best estimate conditions

  1. Kinetic tritium isotopic effects in the position 2 for 5'-hydroxy-L-tryptophane

    International Nuclear Information System (INIS)

    Boroda, E.; Kanska, M.

    2006-01-01

    Tryptophanase converts 5'-hydroxy-L-tryptophane to pyrogronic acid and ammonia, however there are known conditions for the reversed reaction. Mechanism of the processes are not known till now. Kinetic isotopic effect (KIE) permits finding the rate determining stage in the multistage process. In presented communication, 5'-hydroxy-[2- 3 H]-L-tryptophane was synthesized and the KIE in the room temperature determined for different reaction stages

  2. 5-(2-18F-fluoroethoxy)-L-tryptophan as a substrate of system L transport for tumor imaging by PET.

    Science.gov (United States)

    Krämer, Stefanie D; Mu, Linjing; Müller, Adrienne; Keller, Claudia; Kuznetsova, Olga F; Schweinsberg, Christian; Franck, Dominic; Müller, Cristina; Ross, Tobias L; Schibli, Roger; Ametamey, Simon M

    2012-03-01

    Large neutral l-amino acids are substrates of system L amino acid transporters. The level of one of these, LAT1, is increased in many tumors. Aromatic l-amino acids may also be substrates of aromatic l-amino acid decarboxylase (AADC), the level of which is enhanced in endocrine tumors. Increased amino acid uptake and subsequent decarboxylation result in the intracellular accumulation of the amino acid and its decarboxylation product. (18)F- and (11)C-labeled neutral aromatic amino acids, such as l-3,4-dihydroxy-6-(18)F-fluorophenylalanine ((18)F-FDOPA) and 5-hydroxy-l-[β-(11)C]tryptophan, are thus successfully used in PET to image endocrine tumors. However, 5-hydroxy-l-[β-(11)C]tryptophan has a relatively short physical half-life (20 min). In this work, we evaluated the in vitro and in vivo characteristics of the (18)F-labeled tryptophan analog 5-(2-(18)F-fluoroethoxy)-l-tryptophan ((18)F-l-FEHTP) as a PET probe for tumor imaging. (18)F-l-FEHTP was synthesized by no-carrier-added (18)F fluorination of 5-hydroxy-l-tryptophan. In vitro cell uptake and efflux of (18)F-l-FEHTP and (18)F-FDOPA were studied with NCI-H69 endocrine small cell lung cancer cells, PC-3 pseudoendocrine prostate cancer cells, and MDA-MB-231 exocrine breast cancer cells. Small-animal PET was performed with the respective xenograft-bearing mice. Tissues were analyzed for potential metabolites. (18)F-l-FEHTP specific activity and radiochemical purity were 50-150 GBq/μmol and greater than 95%, respectively. In vitro cell uptake of (18)F-l-FEHTP was between 48% and 113% of added radioactivity per milligram of protein within 60 min at 37°C and was blocked by greater than 95% in all tested cell lines by the LAT1/2 inhibitor 2-amino-2-norboranecarboxylic acid. (18)F-FDOPA uptake ranged from 26% to 53%/mg. PET studies revealed similar xenograft-to-reference tissue ratios for (18)F-l-FEHTP and (18)F-FDOPA at 30-45 min after injection. In contrast to the (18)F-FDOPA PET results, pretreatment with the

  3. PD-L2 Regulates B-1 Cell Antibody Production against Phosphorylcholine through an IL-5-Dependent Mechanism.

    Science.gov (United States)

    McKay, Jerome T; Haro, Marcela A; Daly, Christina A; Yammani, Rama D; Pang, Bing; Swords, W Edward; Haas, Karen M

    2017-09-15

    B-1 cells produce natural Abs which provide an integral first line of defense against pathogens while also performing important homeostatic housekeeping functions. In this study, we demonstrate that programmed cell death 1 ligand 2 (PD-L2) regulates the production of natural Abs against phosphorylcholine (PC). Naive PD-L2-deficient (PD-L2 -/- ) mice produced significantly more PC-reactive IgM and IgA. This afforded PD-L2 -/- mice with selectively enhanced protection against PC-expressing nontypeable Haemophilus influenzae , but not PC-negative nontypeable Haemophilus influenzae , relative to wild-type mice. PD-L2 -/- mice had significantly increased PC-specific CD138 + splenic plasmablasts bearing a B-1a phenotype, and produced PC-reactive Abs largely of the T15 Id. Importantly, PC-reactive B-1 cells expressed PD-L2 and irradiated chimeras demonstrated that B cell-intrinsic PD-L2 expression regulated PC-specific Ab production. In addition to increased PC-specific IgM, naive PD-L2 -/- mice and irradiated chimeras reconstituted with PD-L2 -/- B cells had significantly higher levels of IL-5, a potent stimulator of B-1 cell Ab production. PD-L2 mAb blockade of wild-type B-1 cells in culture significantly increased CD138 and Blimp1 expression and PC-specific IgM, but did not affect proliferation. PD-L2 mAb blockade significantly increased IL-5 + T cells in culture. Both IL-5 neutralization and STAT5 inhibition blunted the effects of PD-L2 mAb blockade on B-1 cells. Thus, B-1 cell-intrinsic PD-L2 expression inhibits IL-5 production by T cells and thereby limits natural Ab production by B-1 cells. These findings have broad implications for the development of therapeutic strategies aimed at altering natural Ab levels critical for protection against infectious disease, autoimmunity, allergy, cancer, and atherosclerosis. Copyright © 2017 by The American Association of Immunologists, Inc.

  4. Stress analysis of LOFT containment vessel attachments for the mainsteam and feedwater piping support structures

    International Nuclear Information System (INIS)

    Finicle, D.P.

    1977-01-01

    The LOFT Containment Vessel attachments for the Mainsteam and Feedwater Piping Support Structures have been analyzed for operating and faulted loading conditions. This report contains the analysis of the connections to the containment vessel for the most current design and loading. Also contained in this report is the analysis of the piping supports

  5. LOFT reactor vessel 290/sup 0/ downcomer stalk instrument penetration flange stress analysis

    Energy Technology Data Exchange (ETDEWEB)

    Finicle, D.P.

    1978-06-06

    The LOFT Reactor Vessel 290/sup 0/ Downcomer Stalk Instrument Penetration Flange Stress Analysis has been completed using normal operational and blowdown loading. A linear elastic analysis was completed using simplified hand analysis techniques. The analysis was in accordance with the 1977 ASME Boiler and Pressure Vessel Code, Section III, for a Class 1 component. Loading included internal pressure, bolt preload, and thermal gradients due to normal operating and blowdown.

  6. Pyrazole complexes of rhenium. Synthesis and crystal structure of trans-[Re(O)(OMe)L4]Br2 · L · 4H2O (L - 3,5-dimethylpyrazole)

    International Nuclear Information System (INIS)

    Sokolov, M.N.; Fedorova, N.Eh.; Fedorov, V.E.; Virovets, A.V.; Nun'es, P.

    2002-01-01

    The first rhenium (V) mononuclear complex featuring composition [Re(O)(OMe)L 4 ]Br 2 (L = 3,5-dimethylpyrazole), which is resistant to hydrolysis in neutral aqueous solution, along with its molecular adduct of the composition [Re(O)(OMe)L 4 ]Br 2 · L · 4H 2 O were synthesized. The reaction products were characterized by the methods of elementary analysis, absorption spectroscopy in visible, UV and IR ranges, mass spectrometry. Besides, the adduct structure was studied by the method of X-ray diffraction analysis. It was ascertained that the adduct is crystallized in tetragonal crystal system with unit cell parameters as follows: a = 14.912 (3), c = 17.108 (4) A, sp. gr. I4/m, Z = 4. In the complex molecule linear grouping Re(O)(OMe)] 2+ (Re-O-C angle being equal to 180 deg) is coordinated by four L ligand molecules [ru

  7. LOFT reflood as a function of accumulator initial gas volume

    International Nuclear Information System (INIS)

    Rhodes, H.F.

    1978-01-01

    The effect of the initial gas volume in the LOFT accumulators on the time to start of core reflood, after a LOCA, has been studied. The bases of the calculations are the data used and results presented in the Safety Analysis Report, Rev.1, August 1977, and the data in the RELAP and TOODEE2 program input and output listings. The results of this study show that an initial nitrogen volume of 12 cu ft, or more (at 600 psig initial pressure), would cause start of core reflood in time to prevent the cladding temperature from reaching 2200 0 F. The 12 cu ft initial volume will expand from 600 psig, initial pressure, to about 10 psig (containment pressure shortly after start of LOCA is approximately 8 psig) when all ECC liquid has been expelled from the accumulator. This pressure margin is considered too small; the ECC flowrate will be zero before the accumulator is empty

  8. Thermal analysis of LOFT waste gas processing system nitrogen supply for process line purge and blower seal

    International Nuclear Information System (INIS)

    Tatar, G.A.

    1979-01-01

    The LOFT Waste Gas Processing System uses gaseous nitrogen (GN 2 ) to purge the main process line and to supply pressure on the blower labyrinth seal. The purpose of this analysis was to determine the temperature of the GN 2 at the blower seals and the main process line. Since these temperatures were below 32 0 F the heat rate necessary to raise these temperatures was calculated. This report shows that the GN 2 temperatures at the points mentioned above were below 10 0 F. A heat rate into the GN 2 of 389 Watts added at the point where the supply line enters the vault would raise the GN 2 temperature above 32 0 F

  9. Development and assessment of the COBRA/RELAP5 code

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Jae Jun; Ha, Kwi Seok; Sim, Seok Ku

    1997-04-01

    The COBRA/RELAP5 code, a merged version of the COBRA-TF and RELAP5/MOD3.2 codes, has been developed to combine the realistic three-dimensional reactor vessel model of COBRA-TF with RELAP5/MOD3, thus to produce an advanced system analysis code with a multidimensional thermal-hydraulic module. This report provides the integration scheme of the two codes and the results of developmental assessments. These includes single channel tests, manometric flow oscillation problem, THTF Test 105, and LOFT L2-3 large-break loss-of-coolant experiment. From the single channel tests the integration scheme and its implementation were proven to be valid. Other simulation results showed good agreement with the experiments. The computational speed was also satisfactory. So it is confirmed that COBRA/RELAP5 can be a promising tool for analysis of complicated, multidimensional two-phase flow transients. The area of further improvements in the code integration are also identified. This report also serves as a user`s manual for the COBRA/RELAP5 code. (author). 6 tabs., 20 figs., 20 refs.

  10. German Value Set for the EQ-5D-5L.

    Science.gov (United States)

    Ludwig, Kristina; Graf von der Schulenburg, J-Matthias; Greiner, Wolfgang

    2018-06-01

    The objective of this study was to develop a value set for EQ-5D-5L based on the societal preferences of the German population. As the first country to do so, the study design used the improved EQ-5D-5L valuation protocol 2.0 developed by the EuroQol Group, including a feedback module as internal validation and a quality control process that was missing in the first wave of EQ-5D-5L valuation studies. A representative sample of the general German population (n = 1158) was interviewed using a composite time trade-off and a discrete choice experiment under close quality control. Econometric modeling was used to estimate values for all 3125 possible health states described by EQ-5D-5L. The value set was based on a hybrid model including all available information from the composite time trade-off and discrete choice experiment valuations without any exclusions due to data issues. The final German value set was constructed from a combination of a conditional logit model for the discrete choice experiment data and a censored at -1 Tobit model for the composite time trade-off data, correcting for heteroskedasticity. The value set had logically consistent parameter estimates (p German version of EQ-5D-5L representing the preferences of the German population. The study successfully employed for the first time worldwide the improved protocol 2.0. The value set enables the use of the EQ-5D-5L instrument in economic evaluations and in clinical studies.

  11. Loft CIS analysis 2''-LS-118-AB outside containment penetration S5-D

    Energy Technology Data Exchange (ETDEWEB)

    Morton, D.K.

    1978-09-28

    A stress analysis was performed on the 2''-LS-118-AB pipe system outside containment penetration S5-D. Deadweight, thermal expansion, and seismic loads were considered. The results indicate that this piping will meet ASME Section III, Class 2 requirements provided a U-bolt (S4) is installed as indicated in this report.

  12. LOFT advanced densitometer for nuclear loss-of-coolant experiments

    International Nuclear Information System (INIS)

    Johnson, L.O.; Lassahn, G.D.; Wood, D.B.

    1979-01-01

    A ''nuclear hardened'' gamma densitometer, a device which uses radiation attenuation to measure fluid density in the presence of a background radiation field, is described. Data from the nuclear hardened gamma densitometer are acquired by time sampling the coolant fluid piping and fluid attenuated source energy spectrum. The data are used to calculate transient coolant fluid cross sectional average density to analyze transient mass flow and other thermal-hydraulic characteristics during the Loss-of-Fluid Test (LOFT) loss-of-coolant experiments. The nuclear hardened gamma densitometer uses a pulse height analysis or energy discrimination, pulse counting technique which makes separation of the gamma radiation source signal from the reactor generated gamma radiation background noise signal possible by processing discrete pulses which retain their pulse amplitude information

  13. Design of combinatorial libraries for the exploration of virtual hits from fragment space searches with LoFT.

    Science.gov (United States)

    Lessel, Uta; Wellenzohn, Bernd; Fischer, J Robert; Rarey, Matthias

    2012-02-27

    A case study is presented illustrating the design of a focused CDK2 library. The scaffold of the library was detected by a feature trees search in a fragment space based on reactions from combinatorial chemistry. For the design the software LoFT (Library optimizer using Feature Trees) was used. The special feature called FTMatch was applied to restrict the parts of the queries where the reagents are permitted to match. This way a 3D scoring function could be simulated. Results were compared with alternative designs by GOLD docking and ROCS 3D alignments.

  14. Radioactive gas and hydrogen removal after a LOCE at the LOFT Facility

    International Nuclear Information System (INIS)

    McCormick-Barger, J.W.; Sumpter, K.C.

    1979-01-01

    The use of a silver-zeolite halogen adsorber placed in series with a hydrogen catalytic recombiner and a cryogenic noble gas adsorber assembly constitutes a waste gas processing system (WGPS) capable of handling hydrogen and fission product gases following a Loss-of-Coolant Experiment (LOCE). This paper describes: the types and quantities of gases expected to be found at the facility after a failed-fuel LOCE; the purpose of the WGPS; and the general configuration and expected decontamination factors associated with the LOFT WGPS

  15. PELATIHAN MOULD LOFT (LANTAI GAMBAR KAPAL BAGI PENGRAJIN/PEMBUAT KAPAL TRADISIONAL DI KABUPATEN BATANG - JAWA TENGAH

    Directory of Open Access Journals (Sweden)

    Parlindungan Manik

    2015-08-01

    Full Text Available Kabupaten Batang yang merupakan wilayah pesisir mempunyai banyak potensi yang dapat dikembangkan menjadi aset yang berharga bagi bangsa yaitu para pengrajin/pembuat kapal tradisional. Para pengrajin ini mampu menghasilkan kapal tipe mini purse seine yang mampu berlayar dalam radius pelayaran 12 sampai dengan 200 mil laut. Kapal mini purse siene tipe Batang mempunyai karakteristik yang cukup baik karena mempunyai stabilitas kapal yang baik dan mesin utama diletakkan di dalam kamar mesin (inboard engine. Kapal purse seine ini telah dibuat dan diproduksi secara turun-temurun dan jumlahnya sudah banyak di daerah Batang. Ukuran kapal yang diproduksi mulai dari 5 GT (Gross Tonage sampai dengan 40 GT dengan harga sebesar 300 juta sampai dengan 2 miliar rupiah. Para pembuat kapal pada umumnya tidak atau kurang memiliki pengetahuan tentang gambar desain kapal dari kapal yang diproduksi. Belakangan ini telah dimulai oleh para pengguna yang mensyaratkan adanya gambar desain dan gambar – gambar kerja lainnya sehingga dituntut adanya tenaga kerja dan para pembuat kapal yang mampu membuat dan membaca gambar kapal. Pada Program IbM ini dilaksanakan di CV. Laksana Abadi dan dan CV. Rizki Maulana Bahari yang bertujuan agar para pengrajin/pembuat kapal kayu tradisional di kabupaten Batang memiliki ketrampilan untuk menggambar bentuk badan kapal dalam skala 1:1 pada lantai gambar atau biasa disebut dengan istilah Mould Loft. Dengan tergambarnya bentuk badan kapal/konstruksi kapal dalam skala 1:1 maka akan didapat bentuk badan kapal yang akurat dan ukuran konstruksi kapal yang tepat, sehingga dalam proses pembangunannya segala ukuran yang terpakai sudah tepat dan tidak ada kesalahan bentuk maupun ukuran. Hal ini sangat diperlukan oleh pihak pelaksana, untuk menunjang kemudahan pelaksanaan dan kualitas hasil pekerjaan. Pendekatan yang diterapkan dalam penerapan teknologi ini harus mencakup dua buah aspek yaitu : aspek kognitif dan psikomotorik. Pada aspek kognitif

  16. Radiological observation of the spondylolisthesis: The comparison between L4-L5 and L5-S1 spondylolisthesis in plain film and myelographic of findings

    Energy Technology Data Exchange (ETDEWEB)

    Bae, K. S.; Jo, H. G.; Chung, M. C.; Choi, D. L.; Kim, K. J. [Soonchunhyang University College of Medicine, Seoul (Korea, Republic of)

    1983-12-15

    Spondylolisthesis is displacement of one vertebra upon the other with bony defect of neural arch or elongation of the pars interarticularis. Radiological findings of 35 confirmed cases of spondylolisthesis on plain film and myelogram were reviewed. We also compared the size and contour of slipped vertebra, and myelographic findings between L4-L5 (12 cases) and L5-S1 (23 cases) listhesis. The results were as follows: 1. Average of posterior wedging index of the body, foreward displacement, narrowing of intervertebral disc space and the degenerative changes are more severe in L5-S1 spondylolisthesis. 2. Hyperplastic changes of slipped vertebra are more severe in L5-S1 listhesis. 3. Incidence of spina bifida is not co-related between L4-L5 and L5-SI listhesis. 4. Arthrosis of the intervertebral joint appears both below and above the level of L5-S1 listhesis, but rare in L4-L5 listhesis. 5. The L5 root seems to be the one most often affected in lumber spondylolisthesis on myelogram. 6. The diagnosis of intervertebral disc herniation is less relable in patient with listhesis than in patients without listhesis.

  17. LOFT ECC Pitot Tube and Thermocouple Rake Penetration thermal analysis

    International Nuclear Information System (INIS)

    Tolan, B.J.

    1977-01-01

    A thermal analysis of the LOFT ECC Pitot Tube and Thermocouple Rake Penetration was performed using COUPLE, a two-dimensional finite element computer code. Four transients which conservatively cover all transients the rake will be exposed to were included in this analysis in order to comply with the ASME Code Section III requirements. The transients conservatively cover hot and cold leg operation, and nuclear and nonnuclear operation. The four transients include the LOCE with ECC injection transient, the single control rod drop transient, the scram transient, and the heatup with 0 to 100% load change transient. Temperature distributions in the rake were obtained for each of the four transients and several plots of node temperatures vs. time are given

  18. Corrosion inhibition of iron in 0.5 mol L-1 H2SO4 by halide ions

    Directory of Open Access Journals (Sweden)

    Jeyaprabha C.

    2006-01-01

    Full Text Available The inhibition effect of halide ions such as iodide, bromide and chloride ions on the corrosion of iron in 0.5 mol L-1 H2SO4 and the adsorption behaviour of these ions on the electrode surface have been studied by polarization and impedance methods. It has been found that the inhibition of nearly 90% has been observed for iodide ions at 2.5 10-3 mol L-1, for bromide ions at 10 10-3 mol L-1 and 80% for chloride ions at 2.5 10-3 mol L-1. The inhibition effect is increased with increase of halide ions concentration in the case of I- and Br- ions, whereas it has decreased in the case of Cl- ion at concentrations higher than 5 10-3 mol L-1. The double layer capacitance values have decreased considerably in the presence of halide ions which indicate that these anions are adsorbed on iron at the corrosion potential.

  19. Comparing the UK EQ-5D-3L and English EQ-5D-5L Value Sets.

    Science.gov (United States)

    Mulhern, Brendan; Feng, Yan; Shah, Koonal; Janssen, Mathieu F; Herdman, Michael; van Hout, Ben; Devlin, Nancy

    2018-06-01

    Three EQ-5D value sets (EQ-5D-3L, crosswalk, and EQ-5D-5L) are now available for cost-utility analysis in the UK and/or England. The value sets' characteristics differ, and it is important to assess the implications of these differences. The aim of this paper is to compare the three value sets. We carried out analysis comparing the predicted values from each value set, and investigated how differences in health on the descriptive system is reflected in the utility score by assessing the value of adjacent states. We also assessed differences in values using data from patients who completed both EQ-5D-3L and EQ-5D-5L. The distribution of the value sets systematically differed. EQ-5D-5L values were higher than EQ-5D-3L/crosswalk values. The overall range and difference between adjacent states was smaller. In the patient data, the EQ-5D-5L produced higher values across all conditions and there was some evidence that the value sets rank different health conditions in a similar severity order. There are important differences between the value sets. Due to the smaller range of EQ-5D-5L values, the possible change in quality-adjusted life years (QALYs) might be reduced, but they will apply to both control and intervention groups, and will depend on whether the gain is in quality of life, survival, or both. The increased sensitivity of EQ-5D-5L may also favour QALY gains even if the changes in utility are smaller. Further work should assess the impact of the different value sets on cost effectiveness by repeating the analysis on clinical trial data.

  20. Class I review of LOFT steam generator stress and fatigue life analysis report

    International Nuclear Information System (INIS)

    Fors, R.M.; Silverman, S.

    1977-01-01

    Review of the LOFT steam generator stress and fatigue life analysis report is presented. Deficiencies were found which will require evaluation and in some areas reanalysis. The effects of these deficiencies upon the steam generator will include: to further reduce the allowable ΔP across the tubesheet for the abnormal design case of pressure on primary; and to reduce the allowable number of LOCE transients at some locations of the steam generator from the numbers listed in the stress report and to increase them at other locations

  1. Current applications of optimal estimation and control theory to the LOFT reactor plant

    International Nuclear Information System (INIS)

    Feeley, J.J.; Tylee, J.L.

    1980-01-01

    Two advanced estimation and control systems being developed for the LOFT reactor plant are described and evaluated. The advanced protection system, based on a Kalman filter estimator is capable of providing on-line estimates of such critical variables as fuel and cladding temperature, DNBR, and LHGR. The steam generator LQG control system provides stable, closed-loop, zero steady state error control over a wide power range and also provides on-line estimates of certain unmeasureable variables as steam generator power output and cooling capacity for operator information

  2. Ejection and Lofting of Dust from Hypervelocity Impacts on the Moon

    Science.gov (United States)

    Hermalyn, B.; Schultz, P. H.

    2011-12-01

    Hypervelocity impact events mobilize and redistribute fine-grained regolith dust across the surfaces of planetary bodies. The ejecta mass-velocity distribution controls the location and emplacement of these materials. The current flux of material falling on the moon is dominated by small bolides and should cause frequent impacts that eject dust at high speeds. For example, approximately 25 LCROSS-sized (~20-30m diameter) craters are statistically expected to be formed naturally on the moon during any given earth year. When scaled to lunar conditions, the high-speed component of ejecta from hypervelocity impacts can be lofted for significant periods of time (as evidenced by the LCROSS mission results, c.f., Schultz, et al., 2010, Colaprete, et al., 2010). Even at laboratory scales, ejecta can approach orbital velocities; the higher impact speeds and larger projectiles bombarding the lunar surface may permit a significant portion of material to be launched closer to escape velocity. When these ejecta return to the surface (or encounter local topography), they impact at hundreds of meters per second or faster, thereby "scouring" the surface with low mass oblique impacts. While these high-speed ejecta represent only a small fraction of the total ejected mass, the lofting and subsequent ballistic return of this dust has the highest mobilization potential and will be directly applicable to the upcoming LADEE mission. A suite of hypervelocity impact experiments into granular materials was performed at the NASA Ames Vertical Gun Range (AVGR). This study incorporates both canonical sand targets and air-fall pumice dust to simulate the mechanical properties of lunar regolith. The implementation of a Particle Tracking Velocimetry (PTV) technique permits non-intrusive measurement of the ejecta velocity distribution within the ejecta curtain by following the path of individual ejecta particles. The PTV system developed at the AVGR uses a series of high-speed cameras (ranging

  3. Multilevel Thoracolumbar Spondylolysis with Spondylolisthesis at L4 on L5.

    Science.gov (United States)

    Kim, Whoan Jeang; Song, Young Dong; Choy, Won Sik

    2015-09-01

    A 24-year-old male patient was initially evaluated for persistent back pain. The visual analogue scale (VAS) score was 7 points. Physical examination revealed a decreased range of lumbar spinal motion, which caused pain. Simple X-ray revealed Meyerding grade 1 spondylolisthesis at L4 on L5, with mild dome-shaped superior endplate and consecutive multilevel spondylolysis at T12-L5. Standing anteroposterior and lateral views of the entire spine revealed normal balance of sagittal and coronal alignment. A computed tomography scan revealed bilateral spondylolysis at T12-L4, left unilateral spondylolysis at L5, and spina bifida at L5 to sacral region. Magnetic resonance imaging revealed mild dural ectasia at the lumbar region. Due to the absence of any neurological symptoms, the patient was managed conservatively. He was rested a few weeks with corset brace and physiotherapy. After treatment, his back pain improved, VAS score changed from 7 to 2, and he was able to return to normal activity.

  4. An Efficient One-Pot Synthesis of Pyrano[3,2-c]quinolin-2,5-dione Derivatives Catalyzed by L-Proline

    Directory of Open Access Journals (Sweden)

    Jing Wang

    2012-11-01

    Full Text Available A series of 4-aryl-6-methyl-3,4-dihydro-2H-pyrano[3,2-c]quinolin-2,5(6H-diones were synthesized via the three-component reactions of aromatic aldehydes, 4-hydroxy-1-methylquinolin-2(1H-one, and Meldrum’s acid catalyzed by L-proline. The structures of the products were identified by spectroscopic analysis. A mechanism for this three-component reaction catalyzed by L-proline was proposed.

  5. Current applications of optimal estimation and control theory to the LOFT reactor plant

    International Nuclear Information System (INIS)

    Feeley, J.J.; Tylee, J.L.

    1980-01-01

    Two advanced estimation and control systems being developed for the LOFT reactor plant are described and evaluated. The advanced protection system, based on a Kalman filter estimator is capable of providing on-line estimates of such critical variables as fuel and cladding temperature, DNBR, and LHGR. The steam generator LQG control system provides stable, closed-loop, zero steady state error control over a wide power range and also provides on-line estimates of certain unmeasureable variables as steam generator power output and cooling capacity for operator information. 12 refs

  6. Návrh systému regulace vrtule stálých otáček

    OpenAIRE

    Líška, Lukáš

    2014-01-01

    Diplomová práca sa zaoberá problematikou návrhu elektrickej regulácie vrtule konštantných otáčok. Cieľom práce je ukázať postup návrhu regulátora na príklade vrtule DMP-3 pracujúcej s motorom Rotax 912 ULS, využitím vedomostí z mechaniky letu, elektrotechniky a automatizácie. V rámci práce bude poukázané na problematiku riadenia uhla nastavenia vrtule za použitia elektricky riadeného lineárneho aktuátora. Záver práce tvorí porovnanie výhod vrtule konštantných otáčok DMP-3 uvážením jej použiti...

  7. Measurement properties of the EQ-5D-5L compared to the EQ-5D-3L in psoriasis patients.

    Science.gov (United States)

    Poór, Adrienn Katalin; Rencz, Fanni; Brodszky, Valentin; Gulácsi, László; Beretzky, Zsuzsanna; Hidvégi, Bernadett; Holló, Péter; Kárpáti, Sarolta; Péntek, Márta

    2017-12-01

    The purpose of this study is to assess the measurement properties of EQ-5D-5L compared to EQ-5D-3L in psoriasis patients. A cross-sectional survey was carried out at an academic dermatology clinic in Hungary. Psoriasis patients completed the EQ-5D-3L, EQ-5D-5L and Dermatology Life Quality Index (DLQI) questionnaires, and Psoriasis Area and Severity Index (PASI) was assessed. The UK value sets were used to calculate the 3L and 5L index scores. We tested the feasibility, ceiling effect, redistribution properties, the level of inconsistency and informativity (Shannon and Shannon Evenness indices). Spearman's rank-order correlations were performed between EQ-5D, EQ VAS, DLQI and PASI scores. Known-groups validity was evaluated by comparing age groups, clinical subtypes and treatment groups. Mean age of the 238 patients was 47 years, and 36.6% of them received biological therapy. Mean EQ-5D index score was 0.77 (SD: 0.26) with the 3L and 0.84 (SD: 0.19) with the 5L. The overall ceiling effect decreased from 37.1 (3L) to 32.9% (5L). Shannon index improved significantly for most dimensions, but Shannon Evenness index improved only in three dimensions. Compared to the 3L, the 5L version confirmed a better convergent validity with PASI, but not with the DLQI. Known-groups validity was equally demonstrated both for the 5L and 3L. The EQ-5D-5L seems to improve measurement properties by reducing ceiling effects, strengthening correlations with PASI and improving informativity. Follow-up studies are needed to test responsiveness and reliability in psoriasis.

  8. Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric Surgery

    Directory of Open Access Journals (Sweden)

    Reza Rezvani

    2014-01-01

    Full Text Available Objective. Obesity is associated with metabolic dysfunction with sex differences and chronic, low-grade inflammation. We proposed that hepatic expression of immune complement C3 related receptors (C3aR, C5aR, and C5L2 would be associated with pre- or postmenopausal status and metabolic profile in severely obese women. We hypothesized that C5L2/C5aR ratio, potentially influencing the ASP/C5L2 metabolic versus C5a/C5aR immune response, would predict metabolic profiles after weight loss surgery. Materials and Methods. Fasting plasma (hormone, lipid, and enzyme analysis and liver biopsies (RT-PCR gene expression were obtained from 91 women during surgery. Results. Hepatic C5L2 mRNA expression was elevated in pre- versus postmenopausal women (P<0.01 and correlated positively with circulating estradiol, estrone, ApoB, ApoA1, ApoA1/B, waist circumference, age, and LDL-C (all P<0.05. While plasma ASP was lower in pre- versus postmenopausal women (P<0.01, the hepatic C5L2/C5aR mRNA ratio was increased (P<0.001 and correlated positively with estrone (P<0.01 and estradiol (P<0.001 and negatively with circulating ApoB and liver enzymes ALT, AST, and GGT (all P<0.05. Over 12 months postoperatively, liver enzymes in low C5L2/C5aR mRNA ratio group remained higher (ALP and ALT, P<0.05, AST and GGT, P<0.001 2-way-ANOVA. Conclusion. C5L2-C5aR association with other mediators including estrogens may contribute to hepatic metabolic and inflammatory function.

  9. Scaling studies - PWR

    International Nuclear Information System (INIS)

    Sonneck, G.

    1983-05-01

    A RELAP 4/MOD 6 study was made based on the blowdown phase of the intermediate break experiment LOFT L5-1. The method was to set up a base model and to vary parametrically some areas where it is known or suspected that LOFT differs from a commercial PWR. The aim was not to simulate LOFT or a PWR exactly but to understand the influence of the following parameters on the thermohydraulic behaviour of the system and the clad temperature: stored heat in the downcomer (LOFT has rather large filler blocks in this part of the pressure vessel); bypass between downcomer and upper plenum; and core length. The results show that LOFT is prototypical for all calculated blowdowns. As the clad temperatures decrease with decreasing stored energy in the downcomer, increased bypass and increased core length, LOFT results seem to be realistic as long as realistic bypass sizes are considered; they are conservative in the two other areas. (author)

  10. Comparison of immunogenicity and protective efficacy of genital herpes vaccine candidates herpes simplex virus 2 dl5-29 and dl5-29-41L in mice and guinea pigs.

    Science.gov (United States)

    Hoshino, Yo; Pesnicak, Lesley; Dowdell, Kennichi C; Lacayo, Juan; Dudek, Timothy; Knipe, David M; Straus, Stephen E; Cohen, Jeffrey I

    2008-07-29

    A replication-defective herpes simplex virus (HSV)-2 vaccine, dl5-29, which is deleted for two essential early genes, UL5 and UL29, is highly immunogenic and protective in mice and guinea pigs. In a prior study, a derivative of HSV-2 dl5-29 termed dl5-29-41L, which has an additional deletion in UL41 (that encodes the virion-host shut-off protein), was more immunogenic and protective against challenge with wild-type HSV-2 in mice when compared with dl5-29. To determine if deletion of UL41 improves the efficacy of dl5-29 in protecting guinea pigs from HSV-2, animals were immunized with dl5-29, dl5-29-41L, or PBS. The geometric mean neutralizing antibody titers from the dl5-29 and dl5-29-41L recipients were comparable (10(1.97) and 10(2.19), respectively, p=0.15). After intravaginal challenge with wild-type HSV-2, the dl5-29-41L and dl5-29 recipients shed similar titers of HSV-2 from the vagina. Mean acute disease severity scores, numbers of recurrences during 3 months after infection, and latent viral loads in sacral ganglia were similar for dl5-29 and dl5-29-41L (all p values >0.05). dl5-29 and dl5-29-41L completely protected mice from lethal challenge with HSV-2 and induced virus-specific CD8(+) T cells in the spleens of the animals. Thus, dl5-29 was as immunogenic and protective as dl5-29-41L under these conditions. dl5-29 was at least 250,000-fold less virulent than parental virus by intracranial inoculation in healthy mice, and caused no disease in SCID mice. Both dl5-29-41L and dl5-29 are equally effective and immunogenic in guinea pigs, and dl5-29 is very safe in immunocompromised animals.

  11. Characterization of 5-(2- 18F-fluoroethoxy)-L-tryptophan for PET imaging of the pancreas

    OpenAIRE

    Abbas, Ahmed; Beamish, Christine; McGirr, Rebecca; Demarco, John; Cockburn, Neil; Krokowski, Dawid; Lee, Ting-Yim; Kovacs, Michael; Hatzoglou, Maria; Dhanvantari, Savita

    2016-01-01

    Purpose: In diabetes, pancreatic beta cell mass declines significantly prior to onset of fasting hyperglycemia. This decline may be due to endoplasmic reticulum (ER) stress, and the system L amino acid transporter LAT1 may be a biomarker of this process. In this study, we used 5-(2- 18F-fluoroethoxy)-L-tryptophan ( 18F-L-FEHTP) to target LAT1 as a potential biomarker of beta cell function in diabetes. Procedures: Uptake of 18F-L-FEHTP was determined in wild-type C57BL/6 mice by ex vivo biodis...

  12. Simulations of the x-ray imaging capabilities of the silicon drift detectors (SDD) for the LOFT wide-field monitor

    DEFF Research Database (Denmark)

    Evangelista, Y.; Campana, R.; Del Monte, E.

    2012-01-01

    Detector (LAD), carrying an unprecedented effective area of 10 m^2, is complemented by a coded-mask Wide Field Monitor, in charge of monitoring a large fraction of the sky potentially accessible to the LAD, to provide the history and context for the sources observed by LAD and to trigger its observations...... on their most interesting and extreme states. In this paper we present detailed simulations of the imaging capabilities of the Silicon Drift Detectors developed for the LOFT Wide Field Monitor detection plane. The simulations explore a large parameter space for both the detector design and the environmental...

  13. CD5L Promotes M2 Macrophage Polarization through Autophagy-Mediated Upregulation of ID3

    Directory of Open Access Journals (Sweden)

    Lucía Sanjurjo

    2018-03-01

    Full Text Available CD5L (CD5 molecule-like is a secreted glycoprotein that controls key mechanisms in inflammatory responses, with involvement in processes such as infection, atherosclerosis, and cancer. In macrophages, CD5L promotes an anti-inflammatory cytokine profile in response to TLR activation. In the present study, we questioned whether CD5L is able to influence human macrophage plasticity, and drive its polarization toward any specific phenotype. We compared CD5L-induced phenotypic and functional changes to those caused by IFN/LPS, IL4, and IL10 in human monocytes. Phenotypic markers were quantified by RT-qPCR and flow cytometry, and a mathematical algorithm was built for their analysis. Moreover, we compared ROS production, phagocytic capacity, and inflammatory responses to LPS. CD5L drove cells toward a polarization similar to that induced by IL10. Furthermore, IL10- and CD5L-treated macrophages showed increased LC3-II content and colocalization with acidic compartments, thereby pointing to the enhancement of autophagy-dependent processes. Accordingly, siRNA targeting ATG7 in THP1 cells blocked CD5L-induced CD163 and Mer tyrosine kinase mRNA and efferocytosis. In these cells, gene expression profiling and validation indicated the upregulation of the transcription factor ID3 by CD5L through ATG7. In agreement, ID3 silencing reversed polarization by CD5L. Our data point to a significant contribution of CD5L-mediated autophagy to the induction of ID3 and provide the first evidence that CD5L drives macrophage polarization.

  14. Effects of L-cysteine and N-acetyl-L-cysteine on 4-hydroxy-2, 5-dimethyl-3(2H)-furanone (furaneol), 5-(hydroxymethyl)furfural, and 5-methylfurfural formation and browning in buffer solutions containing either rhamnose or glucose and arginine.

    Science.gov (United States)

    Haleva-Toledo, E; Naim, M; Zehavi, U; Rouseff, R L

    1999-10-01

    Solutions of L-cysteine (Cys) and N-acetyl-L-cysteine (AcCys), containing glucose or rhamnose, with or without arginine, were buffered to pH 3, 5, and 7 and incubated at 70 degrees C for 48 h. Cys and AcCys inhibited the formation of (hydroxymethyl)furfural (HMF) from glucose and methylfurfural (MF) from rhamnose under acidic conditions. AcCys inhibited the accumulation of 4-hydroxy-2, 5-dimethyl- 3(2H)-furanone (DMHF, Furaneol) from rhamnose, but Cys, under our experimental conditions, enhanced Furaneol accumulation from rhamnose. Cys and AcCys reacted directly with Furaneol but not with HMF or MF. Both Cys and AcCys inhibited nonenzymatic browning at pH 7. At pH 3, however, Cys reacted with both glucose and rhamnose to produce unidentified compounds that increased the visible absorbency.

  15. Use of FFTBM by signal mirroring for sensitivity study

    International Nuclear Information System (INIS)

    Prošek, Andrej; Leskovar, Matjaž

    2015-01-01

    Highlights: • The fast Fourier transform based tool was applied for a sensitivity analysis. • The calculations of the BEMUSE programme LOFT L2-5 test were used in the study. • The most influential input parameters were identified and their influence quantified. • It was shown that FFTBM-SM is best suited for conducting quick sensitivity analyses. - Abstract: The state of the art best estimate safety analyses for nuclear reactors use best estimate thermal–hydraulic computer codes with an evaluation of the uncertainties to compare the results of calculations with acceptance criteria. The uncertainty quantification is typically accompanied by a sensitivity analysis, in which the influence of the individual contributors to the uncertainty is determined. The objective of the performed study is to demonstrate that the fast Fourier transform based method by signal mirroring (FFTBM-SM) can be very efficiently used for the sensitivity analysis when one parameter is varied at a time. The sensitivity study was conducted for the LOFT L2-5 test, which simulates the large break loss of coolant accident. The LOFT L2-5 test was analysed in the frame of the Organisation for Economic Co-operation and Development (OECD) Best Estimate Methods – Uncertainty and Sensitivity Evaluation (BEMUSE) programme, where each of the 14 participants performed a reference calculation and up to 15 sensitivity runs of the test. The results show that with the FFTBM-SM the analyst can get the time dependent picture of the input parameter influence on the results. The results suggest that FFTBM-SM is especially appropriate for a sensitivity analysis in which several calculations need to be compared

  16. L-shell ionization in Au by O5+ -and Ni5+ -ion impact

    International Nuclear Information System (INIS)

    Goyal, D.P.; Singh, B.P.; Verma, H.R.

    1995-01-01

    The L X-ray production cross sections in gold by 60 to 72 MeV O 5+ ions and 58 to 87 MeV Ni 5+ -ions have been measured. The L-subshell ionization cross sections derived from these experimental results have been compared with the direct ionization theories viz. plane wave Born approximation (PWBA) theory and modified perturbed-stationary-state theory with energy loss, Coulomb deflection and relativistic effects (MECPSSR). A new procedure has been described to account for the change in the yield ratio Lβ 1 /Lβ 2.15 with energy, for Ni 5+ -ion impact on gold. The L sub-shell ionization cross sections have been derived from Lβ 1 , Lβ 4 and Lβ 2.15 lines of the Lβ group in addition to those calculated by the conventional Datz TRY3 technique using Lα, Lγ 1 and Lγ 2.3 X-ray lines. From the shift in the energies of various L X-ray lines and changes in their intensities, 3 and 5 spectator vacancies in the M-and N-shells in gold have been estimated with O 5+ -ion impact while 7, 20 and 4 to 6 spectator vacancies have been inferred in the M-, N- and O-shells respectively with Ni 5+ -ion impact in the energy range of the projectiles undertaken in the present studies. (orig.)

  17. JNK1 Controls Dendritic Field Size in L2/3 and L5 of the Motor Cortex, Constrains Soma Size and Influences Fine Motor Coordination

    Directory of Open Access Journals (Sweden)

    Emilia eKomulainen

    2014-09-01

    Full Text Available Genetic anomalies on the JNK pathway confer susceptibility to autism spectrum disorders, schizophrenia and intellectual disability. The mechanism whereby a gain or loss of function in JNK signaling predisposes to these prevalent dendrite disorders, with associated motor dysfunction, remains unclear. Here we find that JNK1 regulates the dendritic field of L2/3 and L5 pyramidal neurons of the mouse motor cortex (M1, the main excitatory pathway controlling voluntary movement. In Jnk1-/- mice, basal dendrite branching of L5 pyramidal neurons is increased in M1, as is cell soma size, whereas in L2/3, dendritic arborization is decreased. We show that JNK1 phosphorylates rat HMW-MAP2 on T1619, T1622 and T1625 (Uniprot P15146 corresponding to mouse T1617, T1620, T1623, to create a binding motif, that is critical for MAP2 interaction with and stabilization of microtubules, and dendrite growth control. Targeted expression in M1 of GFP-HMW-MAP2 that is pseudo-phosphorylated on T1619, T1622 and T1625 increases dendrite complexity in L2/3 indicating that JNK1 phosphorylation of HMW-MAP2 regulates the dendritic field. Consistent with the morphological changes observed in L2/3 and L5, Jnk1-/- mice exhibit deficits in limb placement and motor coordination, while stride length is reduced in older animals. In summary, JNK1 phosphorylates HMW-MAP2 to increase its stabilization of microtubules while at the same time controlling dendritic fields in the main excitatory pathway of M1. Moreover, JNK1 contributes to normal functioning of fine motor coordination. We report for the first time, a quantitative sholl analysis of dendrite architecture, and of motor behavior in Jnk1-/- mice. Our results illustrate the molecular and behavioral consequences of interrupted JNK1 signaling and provide new ground for mechanistic understanding of those prevalent neuropyschiatric disorders where genetic disruption of the JNK pathway is central.

  18. Subclinical hypothyroidism diagnosed by thyrotropin-releasing hormone stimulation test in infertile women with basal thyroid-stimulating hormone levels of 2.5 to 5.0 mIU/L.

    Science.gov (United States)

    Lee, You-Jeong; Kim, Chung-Hoon; Kwack, Jae-Young; Ahn, Jun-Woo; Kim, Sung-Hoon; Chae, Hee-Dong; Kang, Byung-Moon

    2014-11-01

    To investigate the prevalence of subclinical hypothyroidism (SH) diagnosed by thyrotropin-releasing hormone (TRH) stimulating test in infertile women with basal thyroid-stimulating hormone (TSH) levels of 2.5 to 5.0 mIU/L. This study was performed in 39 infertile women with ovulatory disorders (group 1) and 27 infertile women with male infertility only (group 2, controls) who had basal serum TSH levels of 2.5 to 5.0 mIU/L and a TRH stimulating test. Serum TSH levels were measured before TRH injection (TSH0) and also measured at 20 minutes (TSH1) and 40 minutes (TSH2) following intravenous injection of 400 µg TRH. Exaggerated TSH response above 30 mIU/L following TRH injection was diagnosed as SH. Group 1 was composed of poor responders (subgroup A), patients with polycystic ovary syndrome (subgroup B) and patients with WHO group II anovulation except poor responder or polycystic ovary syndrome (subgroup C). The prevalence of SH was significantly higher in group 1 of 46.2% (18/39) compared with 7.4% (2/27) in group 2 (P=0.001). TSH0, TSH1, and TSH2 levels were significantly higher in group 1 than the corresponding values in group 2 (Pstimulation test had better be performed in infertile women with ovulatory disorders who have TSH levels between 2.5 and 5.0 mIU/L for early detection and appropriate treatment of SH.

  19. Experiment data report for semiscale Mod-1 Test S-01-5 (isothermal blowdown with core resistance simulator)

    International Nuclear Information System (INIS)

    Zender, S.N.; Crapo, H.S.; Jensen, M.F.; Sackett, K.E.

    1975-04-01

    Recorded test data are presented for Test S-01-5 of the semiscale Mod-1 isothermal blowdown test series. Test S-01-5 is one of several semiscale Mod-1 experiments which are counterparts of the LOFT nonnuclear experiments. System hardware is representative of LOFT with the design based on volumetric scaling methods and with initial conditions duplicating those identified for LOFT nonnuclear tests. Test S-01-5 was conducted with the secondary side of the steam generator pressurized with nitrogen gas in order to effectively eliminate heat transfer from the steam generator during blowdown and thereby to investigate the effect on overall system behavior of heat transfer from the steam generator. An orificed structure was used in the pressure vessel to simulate the LOFT core simulator. The test was initiated at isothermal conditions of 2270 psig and 540 0 F by a simulated offset shear of the cold leg broken loop piping. During system depressurization, coolant was injected into the cold leg of the operating loop to simulate emergency core cooling (ECC). Following the blowdown portion of the test, coolant spray was introduced into the pressure suppression tank to determine the response of the pressure suppression system. The uninterpreted data from Test S-01-5 and the reference material needed for future data analysis and test results reporting activities are presented. The data, presented in the form of graphs in engineering units, have been analyzed only to the extent necessary to assure that they are reasonable and consistent. (U.S.)

  20. Regioselective intramolecular ring closure of 2-amino-6-bromo-2,6-dideoxyhexono-1,4-lactones to 5- or 6-membered iminuronic acid analogues:synthesis of 1-deoxymannojirimycin and 2,5-dideoxy-2,5-imino-D-glucitol

    DEFF Research Database (Denmark)

    Malle, Birgitte Mølholm; Lundt, Inge; Wrodnigg, Tanja M.

    2008-01-01

    closure took place by 5-exo attack on the 5,6-epoxide leading to 2,5-dideoxy-2,5-imino-L-gulonic acid (9b), which was reduced to 2,5-dideoxy-2,5-imino-D-glucitol (9c). The method was further applied to 2-amino-6-bromo-2,6-dideoxy-D-galacto- as well as D-talo-1,4-lactones (14 and 15). However, only......-galacto- as well as L-talo-2-amino-6-bromo-2,6-dideoxy-1,4-lactones ent-14 and ent-15, reacted accordingly to give the D-galacto- and L-altro-1,5-iminuronic acid mimetics, (2S,3S,4R,5S)-3,4,5-trihydroxypipecolic acid (2,6-dideoxy-2,6-imino-L-galactonic acid, ent-16) and (2R,3S,4R,5S)-3,4,5-trihydroxypipecolic...

  1. Relationship between a novel polymorphism of the C5L2 gene and coronary artery disease.

    Directory of Open Access Journals (Sweden)

    Ying-Ying Zheng

    Full Text Available BACKGROUND: C5L2 has been demonstrated to be a functional receptor of acylation-stimulating protein (ASP, which is a stimulator of triglyceride synthesis or glucose transport. However, little is known about the variations in the coding region of the C5L2 gene and their association with coronary artery disease (CAD. METHODOLOGY/PRINCIPAL FINDINGS: We identified a novel single nucleotide polymorphism (SNP, 698C>T (P233L, in exon 2 using a polymerase chain reaction direct-sequencing method. This nucleotide change causes the amino-acid order from proline to leucine at codon 233. We examined the role of this SNP for CAD using two independent case-control studies: one was in the Han population (492 CAD patients and 577 control subjects and the other was in the Uygur population (319 CAD patients and 554 control subjects. Heterozygote carriers of the 698CT genotype were more frequent among CAD patients than among controls not only in the Han population (7.3% versus 1.7% but also in the Uygur population (4.7% versus 1.6%. The odds ratio (OR for carriers of the 698CT genotype for CAD was 4.484 (95% confidence interval (CI: 2.197-9.174 in the Han group and 2.989 (95% CI: 1.292-6.909 in the Uygur population. After adjustment of confounding factors such as sex, age, smoking, alcohol consumption, hypertension, diabetes, as well as serum levels of triglyceride, total cholesterol, high-density lipoprotein, the difference remained significant in the Han group (P<0.001, OR = 6.604, 95% CI: 2.776-15.711 and in the Uygur group (P = 0.047, OR = 2.602, 95% CI: 1.015-6.671. CONCLUSION/SIGNIFICANCE: The 698CT genotype of C5L2 may be a genetic maker of CAD in the Han and Uygur population in western China.

  2. Correction to: Comparing the UK EQ-5D-3L and English EQ-5D-5L Value Sets

    NARCIS (Netherlands)

    B. Mulhern (Brendan); Feng, Y. (Yan); K.K. Shah (Koonal); M.F. Janssen (Bas); M. Herdman (Michael); van Hout, B. (Ben); N. Devlin (Nancy)

    2018-01-01

    textabstractThe article Comparing the UK EQ-5D-3L and English EQ-5D-5L Value Sets, written by Brendan Mulhern, Yan Feng, Koonal Shah, Mathieu F. Janssen, Michael Herdman, Ben van Hout, Nancy Devlin was originally published electronically on the publisher’s internet portal (currently SpringerLink) on

  3. Contribution of l-theanine to the formation of 2,5-dimethylpyrazine, a key roasted peanutty flavor in Oolong tea during manufacturing processes.

    Science.gov (United States)

    Guo, Xiangyang; Song, Chuankui; Ho, Chi-Tang; Wan, Xiaochun

    2018-10-15

    l-Theanine, the most abundant amino acid in tea, is widely believed to be associated with the tea taste, however, its contribution to the formation of tea aroma is still unknown. Volatiles were determined and nitrogen-containing compounds formed during manufacturing processes were quantified. Lower levels of total sugar and l-theanine were detected in the Oolong tea product undergoing full fire processing (FFOT) suggesting that l-theanine probably involved in the volatile formation during manufacturing processes. Methylpyrazine and 2,5-dimethylpyrazine, two newly formed compounds in FFOT, together with other volatiles were successfully detected in a model thermal reaction of d-glucose and l-theanine (GT-MTR) but not detectable in thermal reactions with single d-glucose (G-MTR) or l-theanine (T-MTR). The concentration of 2,5-dimethylpyrazine increased significantly by adding additional l-theanine to 2nd roasted tea. Our study demonstrated that l-theanine, at least partly, contributed to the formation of 2,5-dimethylpyrazine, a key roasted peanutty flavor in Oolong tea. Copyright © 2018. Published by Elsevier Ltd.

  4. Neurocognitive Development and Predictors of L1 and L2 Literacy Skills in Dyslexia: A Longitudinal Study of Children 5-11 Years Old.

    Science.gov (United States)

    Helland, Turid; Morken, Frøydis

    2016-02-01

    The aim of this study was to find valid neurocognitive precursors of literacy development in first language (L1, Norwegian) and second language (L2, English) in a group of children during their Pre-literacy, Emergent Literacy and Literacy stages, by comparing children with dyslexia and a typical group. Children who were 5 years old at project start were followed until the age of 11, when dyslexia was identified and data could be analysed in retrospect. The children's neurocognitive pattern changed both by literacy stage and domain. Visuo-spatial recall and RAN appeared as early precursors of L1 literacy, while phonological awareness appeared as early precursor of L2 English. Verbal long term memory was associated with both L1 and L2 skills in the Literacy stage. Significant group differences seen in the Pre-literacy and Emergent literacy stages decreased in the Literacy stage. The developmental variations by stage and domain may explain some of the inconsistencies seen in dyslexia research. Early identification and training are essential to avoid academic failure, and our data show that visuo-spatial memory and RAN could be suitable early markers in transparent orthographies like Norwegian. Phonological awareness was here seen as an early precursor of L2 English, but not of L1 Norwegian. © 2015 The Authors. Dyslexia published by John Wiley & Sons Ltd.

  5. L5 vertebrectomy for the surgical treatment of tumoral and traumatic lesions of L5 vertebra

    Directory of Open Access Journals (Sweden)

    Tuncay Kaner

    2012-02-01

    Full Text Available We retrospectively reviewed the clinical characteristics and the surgical results of seven patients treated with L5 vertebrectomy. The pathologies, clinical characteristics, preoperative and postoperative radiological findings, surgical techniques, and instrumentation for seven patients operated on between 1998 and 2009 are presented in this article. Biopsies were performed on all patients except those involving trauma. Patients were followed up at three-month intervals in the first year, at 6- month intervals in the second year, and on a regular basis afterward. One patient had a traumatic L5 burst fracture; the other six had tumoral pathologies in the L5 vertebrae. One tumoral lesion was a chordoma, another was a hemangioma, and the remaining four were metastatic lesions. Radiotherapy and chemotherapy were performed for the metastatic tumor patients during the postoperative period. Patients with renal cancer and chordoma survived for 3 years; patients with lung cancer and bladder cancer survived for 1 year; and patients with breast cancer survived for 16 months. The lumbosacral region presents significant stabilization problems because of the presence of sacral slope. In our opinion, if the lesion involves only the L5 vertebra, anterior cage-filled bone cement or bone graft should be performed, as dictated by the pathology and posterior transpedicular instrumentation. If the lesion involves the L4 vertebra or the sacrum and the L5 vertebra, the instrumentation can be extended to cover other segments with sacral attachments. The present cases involved only L5 vertebra and treatment with short-segment stabilization covering the anterior and posterior columns.

  6. In-vitro investigations of the speed of pyrrole formation of 2,5-hexanedione and 2,5-heptanedione with N{alpha}-acetyl-L-lysine as a precondition for a comparative assessment of the neurotoxic potentials of the two {gamma}-diketones; In-vitro-Untersuchungen zur Pyrrolbildungsgeschwindigkeit von 2,5-Hexandion und 2,5-Heptandion mit N{alpha}-Acetyl-L-lysin als Voraussetzung fuer eine vergleichende Abschaetzung der neurotoxischen Potentiale beider {gamma}-Diketone

    Energy Technology Data Exchange (ETDEWEB)

    Richter, M.F.

    1997-09-01

    N-hexane and n-heptane are important solvents. Chronic exposure to n-hexane causes polyneuropathies, which are attributed to the metabolite 2,5-hexanedione, a {gamma} diketone. As a basis for a comparative assessment of the neurotoxic potentials of 2,5-hexanedione and 2,5-heptanedione, an in-vitro test was developed and used to investigate the speed of pyrrole formation of the two {gamma} diketones in reacting with the {epsilon} amino group of N{alpha}-acetyl L-lysine. The speed of the formation of pyrrole was always directly proportional to the respective reactant concentration. It consequently is subject to a second-order kinetics. As a further result, the pyrrole formation speed of 2,5-heptanedione was found to be only half that of 2,5-hexanedione. The results lead to the conclusion that 2,5-heptanedione poses a smaller risk of developing peripheral neuropathy than 2,5-hexanedione. (orig./MG) [Deutsch] n-Hexan und n-Heptan sind wichtige Loesungsmittel. Chronische Exposition gegenueber n-Hexan ruft Polyneuropathien hervor, die auf den Metaboliten 2,5-Hexandion, ein {gamma}-Diketon, zurueckgefuehrt werden. Als Grundlage fuer eine vergleichende Abschaetzung der neurotoxischen Potentiale von 2,5-Hexandion und 2,5-Heptandion wurde in der vorliegenden Arbeit ein In-vitro-Test entwickelt, mit dem die Pyrrolbildungsgeschwindigkeiten der beiden {gamma}-Diketone mit der {epsilon}-Aminogruppe von N{alpha}-Acetyl-L-Iysin untersucht wurden. Die Pyrrolbildungsgeschwindigkeit war stets direkt proportional zur jeweiligen Reaktantenkonzentration. Somit unterliegt sie einer Kinetik 2. Ordnung. Weiterhin wurde gezeigt, dass die Pyrrolbildungsgeschwindigkeit fuer 2,5-Heptandion nur etwa halb so gross ist wie fuer 2,5-Hexandion. Aus den Ergebnissen wird gefolgert, dass das von 2,5-Heptandion ausgehende Risiko an peripheren Neuropathien zu erkranken geringer ist, als das von 2,5-Hexandion ausgehende. (orig./MG)

  7. Application of analytical capability to predict rapid cladding cooling and quench during the blowdown phase of a large break loss-of-coolant accident

    International Nuclear Information System (INIS)

    Aksan, S.N.; Tolman, E.L.; Nelson, R.A.

    1983-01-01

    Large-break Experiments L2-2 and L2-3 conducted in the Loss-of-Fluid Test (LOFT) facility experienced core-wide rapid quenches early in the blowdown transients. To further investigate rapid cladding quenches, separate effects experiments using Semiscale solid-type electric heater rods were conducted in the LOFT Test Support Facility (LTSF) over a wide range of inlet coolant conditions. The analytical capability to predict the cladding temperature response from selected LTSF experiments estimated to bound the hydraulic conditions causing the LOFT early blowdown quenches was investigated using the RELAP4 computer code and was shown to be acceptable over the film boiling cooldown phase. This analytical capability was then used to investigate the behavior of nuclear fuel rods under the same hydraulic conditions. The calculations show that, under rapid cooling conditions, the behaviors of nuclear and electrical heater rods are significantly different because the nuclear rods are conduction limited, while the electrical rods are convection limited

  8. L. Sumera: Symphony No. 5 / Michael Scott Rohan

    Index Scriptorium Estoniae

    Rohan, Michael Scott

    1997-01-01

    L. Sumera: Symphony No. 5; Music for Chamber Orchestra, In memorian. Malmö Symphony Orchestra / Paavo Järvi. BIS CD-770. 64-35 DDD; Various. Searching for Roots - music from Estonia. Virgin VC 5 43242 2; 71: 34 DDD

  9. Characterization of 5-(2-18F-fluoroethoxy-L-tryptophan for PET imaging of the pancreas [version 2; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Ahmed Abbas

    2016-11-01

    Full Text Available Purpose: In diabetes, pancreatic beta cell mass declines significantly prior to onset of fasting hyperglycemia. This decline may be due to endoplasmic reticulum (ER stress, and the system L amino acid transporter LAT1 may be a biomarker of this process. In this study, we used 5-(2-18F-fluoroethoxy-L-tryptophan (18F-L-FEHTP to target LAT1 as a potential biomarker of beta cell function in diabetes. Procedures: Uptake of 18F-L-FEHTP was determined in wild-type C57BL/6 mice by ex vivo biodistribution. Both dynamic and static positron emission tomography (PET images were acquired in wild-type and Akita mice, a model of ER stress-induced diabetes, as well as in mice treated with streptozotocin (STZ. LAT1 expression in both groups of mice was evaluated by immunofluorescence microscopy. Results: Uptake of 18F-L-FEHTP was highest in the pancreas, and static PET images showed highly specific pancreatic signal. Time-activity curves showed significantly reduced 18F-L-FEHTP uptake in Akita mice, and LAT1 expression was also reduced. However, mice treated with STZ, in which beta cell mass was reduced by 62%, showed no differences in 18F-L-FEHTP uptake in the pancreas, and there was no significant correlation of 18F-L-FEHTP uptake with beta cell mass. Conclusions: 18F-L-FEHTP is highly specific for the pancreas with little background uptake in kidney or liver. We were able to detect changes in LAT1 in a mouse model of diabetes, but these changes did not correlate with beta cell function or mass. Therefore, 18F-L-FEHTP PET is not a suitable method for the noninvasive imaging of changes in beta cell function during the progression of diabetes.

  10. l-cysteine desulfhydrase-related H2 S production is involved in OsSE5-promoted ammonium tolerance in roots of Oryza sativa.

    Science.gov (United States)

    Guo, Hongming; Zhou, Heng; Zhang, Jing; Guan, Wenxue; Xu, Sheng; Shen, Wenbiao; Xu, Guohua; Xie, Yanjie; Foyer, Christine Helen

    2017-09-01

    Previous studies revealed that rice heme oxygenase PHOTOPERIOD SENSITIVITY 5 (OsSE5) is involved in the regulation of tolerance to excess ammonium by enhancing antioxidant defence. In this study, the relationship between OsSE5 and hydrogen sulfide (H 2 S), a well-known signalling molecule, was investigated. Results showed that NH 4 Cl triggered the induction of l-cysteine desulfhydrase (l-DES)-related H 2 S production in rice seedling roots. A H 2 S donor not only alleviated the excess ammonium-triggered inhibition of root growth but also reduced endogenous ammonium, both of which were aggravated by hypotaurine (HT, a H 2 S scavenger) or dl-propargylglycine (PAG, a l-DES inhibitor). Nitrogen metabolism-related enzymes were activated by H 2 S, thus resulting in the induction of amino acid synthesis and total nitrogen content. Interestingly, the activity of l-DES, as well as the enzymes involved in nitrogen metabolism, was significantly increased in the OsSE5-overexpression line (35S:OsSE5), whereas it impaired in the OsSE5-knockdown mutant (OsSE5-RNAi). The application of the HT/PAG or H 2 S donor could differentially block or rescue NH 4 Cl-hyposensitivity or hypersensitivity phenotypes in 35S:OsSE5-1 or OsSE5-RNAi-1 plants, with a concomitant modulation of nitrogen assimilation. Taken together, these results illustrated that H 2 S function as an indispensable positive regulator participated in OsSE5-promoted ammonium tolerance, in which nitrogen metabolism was facilitated. © 2017 John Wiley & Sons Ltd.

  11. Decompressive L5 Transverse Processectomy for Bertolotti's Syndrome: A Preliminary Study.

    Science.gov (United States)

    Ju, Chang Il; Kim, Seok Won; Kim, Jong Gyue; Lee, Seung Myung; Shin, Ho; Lee, Hyeun Young

    2017-09-01

    Bertolotti's syndrome is a spinal disorder characterized by abnormal enlargement of the transverse process of the most caudal lumbar vertebra. The L5 transverse process may be enlarged either unilaterally or bilaterally and may articulate or fuse with the sacrum or ilium. Pseudoarticulation between the transverse process of the L5 and the alar of the sacrum can cause buttock pain and leg pain. In addition, the L4 exiting nerve root could be compressed by an enlarged L5 transverse process. The authors could have obtained satisfactory results from the selected cases of Bertolotti's syndrome by applying a selective transverse processectomy of the L5. The objective of this study is to determine the effectiveness of L5 transverse processectomy for symptomatic Bertolotti's syndrome. A retrospective study. A total of 256 patients with Bertolotti's syndrome who had severe buttock pain and unilateral or bilateral radiating leg pain were selected. The correct diagnosis was made based on imaging studies which included computed tomography (CT), plain x-rays, and magnetic resonance imaging (MRI). The final diagnosis was made by confirming pain relief from anesthetic block. A total of 87 patients were classified into 2 groups: group A included 50 patients whose pain was relieved by block into the pseudoarticulation and group B included 37 patients whose pain was relieved by block into the L4 exiting nerve root. A total of 61 cases (group A: 39 cases, group B: 22 cases) were selected as pure L5 transverse processectomy. The primary outcome measures were reduction in pain scores and improvement in quality of life. Among 61 patients, there were 19 men and 42 women. The mean age of the patients was 53.2 ± 12 years (group A: 57.8 ± 14 years [16 - 86 years], group B: 53.4 ± 14 years [27 - 77 years]). The mean follow-up period was 6.5 months. The patients' mean visual analog scale (VAS) prior to surgery was 7.54 ± 0.81 (group A: 7.59 ± 0.93, group B: 7.50 ± 0.86), and the mean

  12. Neurocognitive Development and Predictors of L1 and L2 Literacy Skills in Dyslexia: A Longitudinal Study of Children 5–11 Years Old

    Science.gov (United States)

    Morken, Frøydis

    2015-01-01

    The aim of this study was to find valid neurocognitive precursors of literacy development in first language (L1, Norwegian) and second language (L2, English) in a group of children during their Pre‐literacy, Emergent Literacy and Literacy stages, by comparing children with dyslexia and a typical group. Children who were 5 years old at project start were followed until the age of 11, when dyslexia was identified and data could be analysed in retrospect. The children's neurocognitive pattern changed both by literacy stage and domain. Visuo‐spatial recall and RAN appeared as early precursors of L1 literacy, while phonological awareness appeared as early precursor of L2 English. Verbal long term memory was associated with both L1 and L2 skills in the Literacy stage. Significant group differences seen in the Pre‐literacy and Emergent literacy stages decreased in the Literacy stage. The developmental variations by stage and domain may explain some of the inconsistencies seen in dyslexia research. Early identification and training are essential to avoid academic failure, and our data show that visuo‐spatial memory and RAN could be suitable early markers in transparent orthographies like Norwegian. Phonological awareness was here seen as an early precursor of L2 English, but not of L1 Norwegian. © 2015 The Authors. Dyslexia published by John Wiley & Sons Ltd. PMID:26511662

  13. LOFT facility PSS experiments: analysis of wet well vertical loads resulting from transient initiation

    International Nuclear Information System (INIS)

    Berta, V.T.

    1977-05-01

    Fourteen experiments on the Loss-of-Fluid Test (LOFT) facility pressure suppression system (PSS) are analyzed in relation to the vertical load generated on the suppression tank in the first 0.5 sec of the transient. Variations in principle parameters affecting the generation of vertical loads were included in the experiments. The internal and external vent submergences are identified from the analysis as being parameters which are first order in influencing the magnitude of the vertical load. These parameters are geometric in nature and depend only on PSS design. Physical parameters of total energy input and rate of energy input to the dry well, which influence the dry well pressurization, also are identified as being first order in influencing the magnitude of the vertical loads. The vertical load magnitude is a direct function of these geometric and physical parameters. The analysis indicates that a small value in any one of the parameters will cause the vertical load to be small and to have little dependence on the magnitude of the other parameters. In addition, the phenomena of nonuniform nonsynchronized vent inlet pressures, which have origins that are either geometric, physical, or a combination of both, act as a significant vertical load reduction mechanism

  14. Study of vacancy decays in the L-shell photoionization of barium in the excitation energy range of 5.6-30 keV: from L{sub 2} edge to energy high above the thresholds of double L-vacancy production

    Energy Technology Data Exchange (ETDEWEB)

    Zou, Y [Key Laboratory of Applied Ion Beam Physics (Chinese Educational Ministry), Fudan University, Shanghai 200433 (China); Oura, M [RIKEN Spring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148 (Japan); Hutton, R [Lund Observatory, SE-221 00 Lund (Sweden); Yamaoka, H [RIKEN Spring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148 (Japan); Takeshima, N [Department of Chemistry and Materials Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585 (Japan); Takahiro, K [Department of Chemistry and Materials Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585 (Japan); Kawatsura, K [Department of Chemistry and Materials Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585 (Japan); Mukoyama, T [Kansai Gaidai University, 16-1 Nakamiyahigashino-cho, Hirakata, Osaka 573-1001 (Japan)

    2006-11-28

    Photoinduced Ba L x-rays were measured, in the excitation energy range of 5.6-30 keV, by using high-brilliance undulator radiation. The obtained intensity ratios, the excitation-energy independent L{beta}{sub 4}/L{beta}{sub 3}, L{eta}/L{beta}{sub 1}, L{iota}/L{alpha}{sub 1,2}, L{beta}{sub 6}/L{alpha}{sub 1,2} and L{beta}{sub 2,15}/L{alpha}{sub 1,2} as well as the excitation-energy dependent L{beta}{sub 1}/L{alpha}{sub 1,2}, L{beta}{sub 3}/L{alpha}{sub 1,2} and L{beta}{sub 3}/L{beta}{sub 1}, were compared with theoretical calculations, in which the calculations were performed by applying various subsets of the L subshell fluorescence yields and Coster-Kronig yields. Deviations of the theoretical calculations from the experimental results call on improvements in theory for the emission rates. We have also surveyed the L{alpha}{sub 1,2} related x-ray hypersatellite lines in the photoinduced Ba L x-ray spectrum.

  15. New perovskite-based manganite Pb2Mn2O5

    International Nuclear Information System (INIS)

    Hadermann, Joke; Abakumov, Artem M.; Perkisas, Tyche; D'Hondt, Hans; Tan Haiyan; Verbeeck, Johan; Filonenko, Vladimir P.; Antipov, Evgeny V.; Van Tendeloo, Gustaaf

    2010-01-01

    A new perovskite based compound Pb 2 Mn 2 O 5 has been synthesized using a high pressure high temperature technique. The structure model of Pb 2 Mn 2 O 5 is proposed based on electron diffraction, high angle annular dark field scanning transmission electron microscopy and high resolution transmission electron microscopy. The compound crystallizes in an orthorhombic unit cell with parameters a=5.736(1) A∼√2a p , b=3.800(1) A∼a p , c=21.562(6) A∼4√2a p (a p -the parameter of the perovskite subcell) and space group Pnma. The Pb 2 Mn 2 O 5 structure consists of quasi two-dimensional perovskite blocks separated by 1/2[110] p (101) p crystallographic shear planes. The blocks are connected to each other by chains of edge-sharing MnO 5 distorted tetragonal pyramids. The chains of MnO 5 pyramids and the MnO 6 octahedra of the perovskite blocks delimit six-sided tunnels accommodating double chains of Pb atoms. The tunnels and pyramidal chains adopt two mirror-related configurations ('left' L and 'right' R) and layers consisting of chains and tunnels of the same configuration alternate in the structure according to an -L-R-L-R-sequence. The sequence is sometimes locally violated by the appearance of -L-L- or -R-R-fragments. A scheme is proposed with a Jahn-Teller distortion of the MnO 6 octahedra with two long and two short bonds lying in the a-c plane, along two perpendicular orientations within this plane, forming a d-type pattern. - Graphical abstract: Order of the Jahn-Teller distorted MnO 6 octahedra in Pb 2 Mn 2 O 5 . Two long and two short bonds lie in the a-c plane, along two perpendicular orientations within this plane, forming a d-type pattern.

  16. Assembly of bicyclic or monocyclic clusters from [(η5-C5Me5)2Mo2(μ3-S)4(CuMeCN)2]2+ with tetraphosphine or N,P mixed ligands: syntheses, structures and enhanced third-order NLO performances.

    Science.gov (United States)

    Ren, Zhi-Gang; Sun, Sha; Dai, Min; Wang, Hui-Fang; Lü, Chun-Ning; Lang, Jian-Ping; Sun, Zhen-Rong

    2011-09-07

    Reactions of the preformed cluster [(η(5)-C(5)Me(5))(2)Mo(2)(μ(3)-S)(4)Cu(2)(MeCN)(2)](ClO(4))(2) (1) with two tetraphosphine ligands, 1,4-N,N,N',N'-tetra(diphenylphosphanylmethyl)benzene diamine (dpppda) and N,N,N',N'-tetra(diphenylphosphanylmethyl)ethylene diamine (dppeda), produced two bicyclic clusters {[(η(5)-C(5)Me(5))(2)Mo(2)(μ(3)-S)(4)Cu(2)](2)(L)}(ClO(4))(4) (3: L = dpppda; 4: L = dppeda). Analogous reactions of 1 or [(η(5)-C(5)Me(5))(2)Mo(2)(μ(3)-S)(4)Cu(2)(MeCN)(2)](PF(6))(2) (2) with two N,P mixed ligands, N,N-bi(diphenylphosphanylmethyl)-2-aminopyridine (bdppmapy) and N-diphenylphosphanylmethyl-4-aminopyridine (dppmapy), afforded two monocyclic clusters {[(η(5)-C(5)Me(5))(2)Mo(2)(μ(3)-S)(4)Cu(2)](2)(L)(2)}X(4) (5: L = bdppmapy, X = ClO(4); 6: L = dppmapy, X = PF(6)). Compounds 3-6 were fully characterized by elemental analysis, IR spectra, UV-vis spectra, (1)H and (31)P{(1)H} NMR spectra, ESI-MS and single-crystal X-ray crystallography. In the tetracations of 3-6, two cubane-like [Mo(2)(μ(3)-S)(4)Cu(2)] cores are linked either by one dpppda or dppeda bridge to form a bicyclic structure or by a pair of bdppmapy or dppmapy bridges to afford a monocyclic structure. The third-order nonlinear optical (NLO) properties of 1 and 3-6 in MeCN were also investigated by femtosecond degenerate four-wave mixing (DFWM) technique with a 50 fs pulse width at 800 nm. Compounds 3-6 exhibited enhanced third-order NLO performances relative to that of 1. This journal is © The Royal Society of Chemistry 2011

  17. Treatment of a Patient after microdiscectomy of L4/L5

    OpenAIRE

    Evangelou, Angela

    2017-01-01

    Title of the thesis: Treatment of a Patient after microdiscectomy of L4/L5 Thesis aim: This thesis involves a case study approach regarding to a patient after microdiscectomy of L4/L5. The thesis is divided into theoretical part which describes the anatomy and physiology of vertebral column and intervertebral disc. It also contains surgical and rehabilitation approach. The second is a health chart which contains the procedures I have done with my patient, all the examinations, conclusions, th...

  18. API 5L X80 PSL2 pipes manufactured by ERW process: TenarisConfab and USIMINAS development; Tubos API 5L X80 PSL2 processados por ERW: desenvolvimento TenarisConfab-Usiminas

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Ronaldo C.; Roza, Juliana E.; Kojima, Sergio S. [TenarisConfab, Pindamonhangaba, SP (Brazil); Miranda, Lucio S.; Turani, Leonardo O. [USIMINAS, Ipatinga, MG (Brazil)

    2005-07-01

    The utilization of high strength steels can decrease, both, installation and operation costs of a new pipeline, since the increase of mechanical resistance can reduce the pipe wall thickness and, consequently, the weight of the pipe. For this purpose, the manufacture of electric resistance welding (ERW) pipes is recent, and it comes as an opportunity for gas pipelines, mainly onshore projects. As a function of their larger productivity, ERW pipes are more economically attractive than those produced by seamless and SAW processes. TenarisConfab and USIMINAS, through studies and investments in manufacturing processes, have been working to increase their skills, in order to supply ERW pipes in API 5L X80 PSL2 steel. The steel elaborated by USIMINAS is low C, high Mn, Nb, V and Ti microalloyed, and with additions of Cr and Mo, aiming to produce hot rolled coils with acicular microstructure, achieved as a combination between its chemical composition and higher cooling rates after hot rolling. The results in {phi}18''(457 mm) x 0,469''(11,91 mm) pipes are presented in this article. High toughness values reached at very low temperatures suggest that ERW API 5L X80 PSL2 pipes developed by TenarisConfab and USIMINAS may be an excellent option of choice for onshore projects that request pipes in diameters up to 20''(508 mm) and thickness up to 0,500'' (12,7 mm). (author)

  19. A Radiographic Measurement of the Anterior Epidural Space at L4-5 Disc Level.

    Science.gov (United States)

    Xu, Rui-Sheng; Wu, Jie-Shi; Lu, Hai-Dan; Zhu, Hao-Gang; Li, Xia; Dong, Jian; Yuan, Feng-Lai

    2017-05-01

    To observe the morphology character of the anterior epidural space at the L 4-5 disc level and to provide an anatomical basis for safely and accurately performing a percutaneous endoscopic lumbar discectomy (PELD). Fifty-five cases with L 5 S 1 lumbar disc herniation were included in this study, and cases with L 4-5 disease were excluded. When the puncture needle reached the epidural space at the L 5 S 1 level, iohexol was injected at the pressure of 50 cm H 2 O during the PELD, then C-Arm fluoroscopy was used to obtain standard lumbar frontal and lateral images. The widths of epidural space at the level of the L 4 lower endplate, the L 5 upper endplate, as well as the middle point of the L 4-5 disc were measured from the lumbar lateral X-ray film. Epidural space at the L 4-5 disc plane performs like a trapezium chart with a short side at the head end and a long side at the tail end in the lumbar lateral X-ray radiograph, while the average widths of epidural space were 10.2 ± 2.5, 12.3 ± 2.3, and 13.8 ± 2.6 mm at the upper, middle, and lower level of the L 4-5 disc. Understanding the morphological characteristics of epidural space will contribute to improving the safety of the tranforaminal percutaneous endoscopy technique. © 2017 Chinese Orthopaedic Association and John Wiley & Sons Australia, Ltd.

  20. Analysis list: Ash2l [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Ash2l Blood + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Ash2l.1.tsv... http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Ash2l.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Ash2...l.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Ash2l.Blood.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Blood.gml ...

  1. MODIS/Aqua Clear Radiance Statistics Indexed to Global Grid 5-Min L2 Swath 10km V006

    Data.gov (United States)

    National Aeronautics and Space Administration — The MODIS/Aqua Clear Radiance Statistics Indexed to Global Grid 5-Min L2 Swath 10km (MYDCSR_G) provides a variety of statistical measures that characterize observed...

  2. Analysis of LOFT pressurizer spray and surge nozzles to include a 4500F step transient

    International Nuclear Information System (INIS)

    Nitzel, M.E.

    1978-01-01

    This report presents the analysis of the LOFT pressurizer spray and surge nozzles to include a 450 0 F step thermal transient. Previous analysis performed under subcontract by Basic Technology Incorporated was utilized where applicable. The SAASIII finite element computer program was used to determine stress distributions in the nozzles due to the step transient. Computer results were then incorporated in the necessary additional calculations to ascertain that stress limitations were not exceeded. The results of the analysis indicate that both the spray and surge nozzles will be within stress allowables prescribed by subsubarticle NB-3220 of the 1974 edition of the ASME Boiler and Pressure Vessel Code when subjected to currently known design, normal operating, upset, emergency, and faulted condition loads

  3. BEHAVIOUR CORROSION OF API 5L X70 AND API 5L X80 STEELS IN A CO2 MEDIUM BY SURFACE ANALYSIS AND ELECTROCHEMICAL TECHNIQUES

    OpenAIRE

    MILAGROS MABEL GUILLEN NUNEZ

    2006-01-01

    A seleção de materiais para o transporte de óleo e gás não é feita pela sua resistência à corrosão, mas sim pelas suas boas propriedades mecânicas, facilidade de fabricação e baixos custos. Devido às taxas de perda de material, causado pela corrosão interna, existe necessidade em se conhecer o comportamento dos aços de mais alta resistência, utilizados especialmente em gasodutos e oleodutos tais como API 5L X70 e API 5L X80. Um dos elementos responsáveis ...

  4. An EQ-5D-5L value set based on Uruguayan population preferences.

    Science.gov (United States)

    Augustovski, Federico; Rey-Ares, Lucila; Irazola, Vilma; Garay, Osvaldo Ulises; Gianneo, Oscar; Fernández, Graciela; Morales, Marcelo; Gibbons, Luz; Ramos-Goñi, Juan Manuel

    2016-02-01

    To derive a value set from Uruguayan general population using the EQ-5D-5L questionnaire and report population norms. General population individuals were randomly assigned to value 10 health states using composite time trade off and 7 pairs of health states through discrete choice experiments. A stratified sampling with quotas by location, gender, age and socio-economic status was used to respect the Uruguayan population structure. Trained interviewers conducted face-to-face interviews. The EuroQol valuation technology was used to administer the protocol as well as to collect the data. OLS and maximum likelihood robust regression models with or without interactions were tested. We included 794 respondents between 20 and 83 years. Their characteristics were broadly similar to the Uruguayan population. The main effects robust model was chosen to derive social values. Values ranged from -0.264 to 1. States with a misery index = 6 had a mean predicted value of 0.965. When comparing the Uruguayan population with the Argentinian EQ-5D-5L crosswalk value set, the prediction for states which differed from full health only in having one of the dimensions at level 2 were about 0.05 higher in Uruguay. The mean index value, using the selected Uruguayan EQ-5D-5L value set, for the general population in Uruguay was 0.895. In general, older people had worse values and males had slightly better values than females. We derived the EQ-5D-5L Uruguayan value set, the first in Latin America. These results will help inform decision-making using economic evaluations for resource allocation decisions.

  5. Experiment data report for Semiscale Mod-1 test S-02-5 (blowdown heat transfer test)

    International Nuclear Information System (INIS)

    1975-12-01

    Recorded test data are presented for Test S-02-5 of the Semiscale Mod-1 blowdown heat transfer test series. Test S-02-5 is one of several Semiscale Mod-1 experiments conducted to investigate the thermal and hydraulic phenomena accompanying a hypothesized loss-of-coolant accident in a water-cooled nuclear reactor system and to provide data for the assessment of the Loss-of-Fluid Test (LOFT) design basis. Test S-02-5 was conducted from an initial cold leg fluid temperature of 544 0 F and an initial pressure of 2,253 psia. A simulated double-ended offset shear cold leg break was used to investigate the system response to a depressurization transient with full core power (1.6 MW). An electrically heated core was used in the pressure vessel to simulate the effects of a nuclear core. System flow was set to achieve the full design core temperature differential of 66 0 F. The flow resistance of the intact loop was based on core area scaling. During system depressurization, core power was reduced from the initial level of 1.6 MW in such a manner as to simulate the surface heat flux response of the LOFT nuclear fuel rods until such time that departure from nucleate boiling occurs

  6. Extracellular histones are essential effectors of C5aR- and C5L2-mediated tissue damage and inflammation in acute lung injury.

    Science.gov (United States)

    Bosmann, Markus; Grailer, Jamison J; Ruemmler, Robert; Russkamp, Norman F; Zetoune, Firas S; Sarma, J Vidya; Standiford, Theodore J; Ward, Peter A

    2013-12-01

    We investigated how complement activation promotes tissue injury and organ dysfunction during acute inflammation. Three models of acute lung injury (ALI) induced by LPS, IgG immune complexes, or C5a were used in C57BL/6 mice, all models requiring availability of both C5a receptors (C5aR and C5L2) for full development of ALI. Ligation of C5aR and C5L2 with C5a triggered the appearance of histones (H3 and H4) in bronchoalveolar lavage fluid (BALF). BALF from humans with ALI contained H4 histone. Histones were absent in control BALF from healthy volunteers. In mice with ALI, in vivo neutralization of H4 with IgG antibody reduced the intensity of ALI. Neutrophil depletion in mice with ALI markedly reduced H4 presence in BALF and was highly protective. The direct lung damaging effects of extracellular histones were demonstrated by airway administration of histones into mice and rats (Sprague-Dawley), which resulted in ALI that was C5a receptor-independent, and associated with intense inflammation, PMN accumulation, damage/destruction of alveolar epithelial cells, together with release into lung of cytokines/chemokines. High-resolution magnetic resonance imaging demonstrated lung damage, edema and consolidation in histone-injured lungs. These studies confirm the destructive C5a-dependent effects in lung linked to appearance of extracellular histones.

  7. Park Forest (L5) and the asteroidal source of shocked L chondrites

    Science.gov (United States)

    Meier, Matthias M. M.; Welten, Kees C.; Riebe, My E. I.; Caffee, Marc W.; Gritsevich, Maria; Maden, Colin; Busemann, Henner

    2017-08-01

    The Park Forest (L5) meteorite fell in a suburb of Chicago, Illinois (USA) on March 26, 2003. It is one of the currently 25 meteorites for which photographic documentation of the fireball enabled the reconstruction of the meteoroid orbit. The combination of orbits with pre-atmospheric sizes, cosmic-ray exposure (CRE), and radiogenic gas retention ages ("cosmic histories") is significant because they can be used to constrain the meteoroid's "birth region," and test models of meteoroid delivery. Using He, Ne, Ar, 10Be, and 26Al, as well as a dynamical model, we show that the Park Forest meteoroid had a pre-atmospheric size close to 180 g cm-2, 0-40% porosity, and a pre-atmospheric mass range of 2-6 tons. It has a CRE age of 14 ± 2 Ma, and (U, Th)-He and K-Ar ages of 430 ± 90 and 490 ± 70 Ma, respectively. Of the meteorites with photographic orbits, Park Forest is the second (after Novato) that was shocked during the L chondrite parent body (LCPB) break-up event approximately 470 Ma ago. The suggested association of this event with the formation of the Gefion family of asteroids has recently been challenged and we suggest the Ino family as a potential alternative source for the shocked L chondrites. The location of the LCPB break-up event close to the 5:2 resonance also allows us to put some constraints on the possible orbital migration paths of the Park Forest meteoroid.

  8. L2 Working Memory Capacity and L2 Reading Skill.

    Science.gov (United States)

    Harrington, Mike; Sawyer, Mark

    1992-01-01

    Examines the sensitivity of second-language (L2) working memory (ability to store and process information simultaneously) to differences in reading skills among advanced L2 learners. Subjects with larger L2 working memory capacities scored higher on measures of L2 reading skills, but no correlation was found between reading and passive short-term…

  9. Metabolic engineering of Escherichia coli to produce 2'-fucosyllactose via salvage pathway of guanosine 5'-diphosphate (GDP)-l-fucose.

    Science.gov (United States)

    Chin, Young-Wook; Seo, Nari; Kim, Jae-Han; Seo, Jin-Ho

    2016-11-01

    2'-Fucosyllactose (2-FL) is one of the key oligosaccharides in human milk. In the present study, the salvage guanosine 5'-diphosphate (GDP)-l-fucose biosynthetic pathway from fucose was employed in engineered Escherichia coli BL21star(DE3) for efficient production of 2-FL. Introduction of the fkp gene coding for fucokinase/GDP-l-fucose pyrophosphorylase (Fkp) from Bacteroides fragilis and the fucT2 gene encoding α-1,2-fucosyltransferase from Helicobacter pylori allows the engineered E. coli to produce 2-FL from fucose, lactose and glycerol. To enhance the lactose flux to 2-FL production, the attenuated, and deleted mutants of β-galactosidase were employed. Moreover, the 2-FL yield and productivity were further improved by deletion of the fucI-fucK gene cluster coding for fucose isomerase (FucI) and fuculose kinase (FucK). Finally, fed-batch fermentation of engineered E. coli BL21star(DE3) deleting lacZ and fucI-fucK, and expressing fkp and fucT2 resulted in 23.1 g/L of extracellular concentration of 2-FL and 0.39 g/L/h productivity. Biotechnol. Bioeng. 2016;113: 2443-2452. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  10. Isolation of eukaryotic ribosomal proteins. Purification and characterization of the 60 S ribosomal subunit proteins L4, L5, L7, L9, L11, L12, L13, L21, L22, L23, L26, L27, L30, L33, L35', L37, and L39.

    Science.gov (United States)

    Tsurugi, K; Collatz, E; Wool, E G; Lin, A

    1976-12-25

    The proteins of the large subunit of rat liver ribosomes were separated into seven groups by stepwise elution from carboxymethylcellulose with LiCl at pH 6.5. Seventeen proteins (L4, L5, L7, L9, L11, L12, L13, L21, L22, L23, L26, L27, L30, L33, L35', L37, and L39) were isolated from three of the groups (B60, D60, G60) by ion exchange chromatography on carboxymethylcellulose and by filtration through Sephadex. The amount of protein obtained varied from 0.5 to 15 mg. Eight of the proteins (L9, L11, L13, L21, L22, L35', L37 and L39) had no detectable contamination; the impurities in the others were no greater than 9%. The molecular weight of the proteins was estimated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate; the amino acid composition was determined.

  11. Lysobacter capsici AZ78 produces cyclo(L-Pro-L-Tyr), a 2,5-diketopiperazine with toxic activity against sporangia of Phytophthora infestans and Plasmopara viticola.

    Science.gov (United States)

    Puopolo, G; Cimmino, A; Palmieri, M C; Giovannini, O; Evidente, A; Pertot, I

    2014-10-01

    To investigate low molecular weight compounds produced in vitro by Lysobacter capsici AZ78 and their toxic activity against sporangia of plant pathogenic oomycetes. Assays carried out in vitro showed that L. capsici AZ78 drastically inhibits the growth of plant pathogenic oomycetes. Accordingly, the preventive application of culture filtrates of L. capsici AZ78 on grapevine and tomato plants reduced the infections, respectively, caused by Plasmopara (Pl.) viticola and Phytophthora infestans. The subsequent chemical analysis of the culture filtrates of L. capsici AZ78 by spectroscopic (essentially 1D and 2D (1)H NMR and (13)C NMR and ESI MS spectra) and optical methods led to the identification of the 2,5-diketopiperazine cyclo(L-Pro-L-Tyr) that inhibited the development of P. infestans sporangia in vitro and on tomato leaves. Furthermore, a genomic region with high sequence identity with genes coding for a hybrid polyketide synthase and nonribosomal peptide synthetase was detected in L. capsici AZ78. Lysobacter capsici AZ78 produces cyclo(L-Pro-L-Tyr) in vitro that was effective in killing the sporangia of P. infestans and Pl. viticola in vitro. Moreover, this low molecular weight compound prevents the occurrence of late blight lesions when applied on tomato leaves. The application of L. capsici AZ78 cells or its own culture filtrates effectively controls both P. infestans and Pl. viticola. Cyclo(L-Pro-L-Tyr) produced by L. capsici AZ78 is toxic against sporangia of both these oomycetes. These data enforce the potential in the use of Lysobacter members for the control of plant pathogenic oomycetes and provide the basis for the development of new low-impact fungicides based on cyclo(L-Pro-L-Tyr). © 2014 The Society for Applied Microbiology.

  12. A model of the effect of uncertainty on the C elegans L2/L2d decision.

    Directory of Open Access Journals (Sweden)

    Leon Avery

    Full Text Available At the end of the first larval stage, the C elegans larva chooses between two developmental pathways, an L2 committed to reproductive development and an L2d, which has the option of undergoing reproductive development or entering the dauer diapause. I develop a quantitative model of this choice using mathematical tools developed for pricing financial options. The model predicts that the optimal decision must take into account not only the expected potential for reproductive growth, but also the uncertainty in that expected potential. Because the L2d has more flexibility than the L2, it is favored in unpredictable environments. I estimate that the ability to take uncertainty into account may increase reproductive value by as much as 5%, and discuss possible experimental tests for this ability.

  13. The first 3':5'-cyclic nucleotide-amino acid complex: L-His-cIMP.

    Science.gov (United States)

    Slepokura, Katarzyna

    2012-08-01

    In the crystal structure of the L-His-cIMP complex, i.e. L-histidinium inosine 3':5'-cyclic phosphate [systematic name: 5-(2-amino-2-carboxyethyl)-1H-imidazol-3-ium 7-hydroxy-2-oxo-6-(6-oxo-6,9-dihydro-1H-purin-9-yl)-4a,6,7,7a-tetrahydro-4H-1,3,5,2λ(5)-furo[3,2-d][1,3,2λ(5)]dioxaphosphinin-2-olate], C(6)H(10)N(3)O(2)(+)·C(10)H(10)N(4)O(7)P(-), the Hoogsteen edge of the hypoxanthine (Hyp) base of cIMP and the Hyp face are engaged in specific amino acid-nucleotide (His···cIMP) recognition, i.e. by abutting edge-to-edge and by π-π stacking, respectively. The Watson-Crick edge of Hyp and the cIMP phosphate group play a role in nonspecific His···cIMP contacts. The interactions between the cIMP anions (anti/C3'-endo/trans-gauche/chair conformers) are realized mainly between riboses and phosphate groups. The results for this L-His-cIMP complex, compared with those for the previously reported solvated L-His-IMP crystal structure, indicate a different nature of amino acid-nucleotide recognition and interactions upon the 3':5'-cyclization of the nucleotide phosphate group.

  14. Synthesis, crystal structure and magnetic studies of tetranuclear hydroxo and ligand bridged [Co4(μ3-OH)22-dea)2(L-L)4]4Cl·8H2O [L-L = 2,2'-bipyridine or 1,10-phenanthroline] complexes with mixed valence defect dicubane core.

    Science.gov (United States)

    Siddiqi, Zafar A; Siddique, Armeen; Shahid, M; Khalid, Mohd; Sharma, Prashant K; Anjuli; Ahmad, Musheer; Kumar, Sarvendra; Lan, Yanhua; Powell, Annie K

    2013-07-14

    X-ray crystallography of the title complexes indicates a discrete mixed valence (Co2(II)-Co2(III)) defect dicubane molecular unit where each cobalt nucleus attains a distorted octahedral geometry. The α-diimine (L-L) chelator coordinated to each cobalt ion stops further polymerization or nuclearization. The water molecules in the lattice play a crucial role in the formation of the supramolecular architectures. Magnetic data were analyzed using the effective spin-1/2 Hamiltonian approach and the parameters are, J = 115(6) K, ΔJ = -57.0(1.2) K, g(xy) = 3.001(25), and g(z) = 7.214(7) for 1 and J = 115(12) K, ΔJ = -58.5(2.5) K, g(xy) = 3.34(5), and g(z) = 6.599(12) for 2 suggesting that only the g matrices are prone to the change of α-diimine chelator.

  15. Varying the Lewis base coordination of the Y2N2 core in the reduced dinitrogen complexes {[(Me3Si)2N]2(L)Y}2(μ-η22-N2) (L = benzonitrile, pyridines, triphenylphosphine oxide, and trimethylamine N-oxide).

    Science.gov (United States)

    Corbey, Jordan F; Farnaby, Joy H; Bates, Jefferson E; Ziller, Joseph W; Furche, Filipp; Evans, William J

    2012-07-16

    The effect of the neutral donor ligand, L, on the Ln(2)N(2) core in the (N═N)(2-) complexes, [A(2)(L)Ln](2)(μ-η(2):η(2)-N(2)) (Ln = Sc, Y, lanthanide; A = monoanion; L = neutral ligand), is unknown since all of the crystallographically characterized examples were obtained with L = tetrahydrofuran (THF). To explore variation in L, displacement reactions between {[(Me(3)Si)(2)N](2)(THF)Y}(2)(μ-η(2):η(2)-N(2)), 1, and benzonitrile, pyridine (py), 4-dimethylaminopyridine (DMAP), triphenylphosphine oxide, and trimethylamine N-oxide were investigated. THF is displaced by all of these ligands to form {[(Me(3)Si)(2)N](2)(L)Y}(2)(μ-η(2):η(2)-N(2)) complexes (L = PhCN, 2; py, 3; DMAP, 4; Ph(3)PO, 5; Me(3)NO, 6) that were fully characterized by analytical, spectroscopic, density functional theory, and X-ray crystallographic methods. The crystal structures of the Y(2)N(2) cores in 2-5 are similar to that in 1 with N-N bond distances between 1.255(3) Å and 1.274(3) Å, but X-ray analysis of the N-N distance in 6 shows it to be shorter: 1.198(3) Å.

  16. Electronegative L5-LDL induces the production of G-CSF and GM-CSF in human macrophages through LOX-1 involving NF-κB and ERK2 activation.

    Science.gov (United States)

    Yang, Tzu-Ching; Chang, Po-Yuan; Kuo, Tzu-Ling; Lu, Shao-Chun

    2017-12-01

    Circulating levels of granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF) are associated with the severity of acute myocardial infarction (AMI). However, what causes increases in G-CSF and GM-CSF is unclear. In this study, we investigated whether L5-low-density lipoprotein (LDL), a mildly oxidized LDL from AMI, can induce G-CSF and GM-CSF production in human macrophages. L1-LDL and L5-LDL were isolated through anion-exchange chromatography from AMI plasma. Human macrophages derived from THP-1 and peripheral blood mononuclear cells were treated with L1-LDL, L5-LDL, or copper-oxidized LDL (Cu-oxLDL) and G-CSF and GM-CSF protein levels in the medium were determined. In addition, the effects of L5-LDL on G-CSF and GM-CSF production were tested in lectin-type oxidized LDL receptor-1 (LOX-1), CD36, extracellular signal-regulated kinase (ERK) 1, and ERK2 knockdown THP-1 macrophages. L5-LDL but not L1-LDL or Cu-oxLDL significantly induced production of G-CSF and GM-CSF in macrophages. In vitro oxidation of L1-LDL and L5-LDL altered their ability to induce G-CSF and GM-CSF, suggesting that the degree of oxidation is critical for the effects. Knockdown and antibody neutralization experiments suggested that the effects were caused by LOX-1. In addition, nuclear factor (NF)-κB and ERK1/2 inhibition resulted in marked reductions of L5-LDL-induced G-CSF and GM-CSF production. Moreover, knockdown of ERK2, but not ERK1, hindered L5-LDL-induced G-CSF and GM-CSF production. The results indicate that L5-LDL, a naturally occurring mild oxidized LDL, induced G-CSF and GM-CSF production in human macrophages through LOX-1, ERK2, and NF-κB dependent pathways. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Quality of life profile and psychometric properties of the EQ-5D-5L in HIV/AIDS patients

    Directory of Open Access Journals (Sweden)

    Tran Bach

    2012-11-01

    Full Text Available Abstract Objectives We assessed health-related quality of life (HRQOL, its associated factors, and examined measurement properties of the EuroQol - 5 Dimensions - 5 Levels (EQ-5D-5L in HIV/AIDS patients. Methods A cross-sectional multi-site survey was conducted in 1016 patients (age: 35.4 ± 7.0 years; 63.8% male in three epicenters of Vietnam. Internal consistency reliability, convergent validity, and discriminative validity of the EQ-5D-5L and a visual analogue scale (VAS were evaluated. Tobit censored regression models were used to identify predictors of HRQOL in HIV/AIDS patients. Results The mean EQ-5D-5L single index and VAS were 0.65 (95% Confidence Interval (CI = 0.63; 0.67 and 70.3 (95% CI = 69.2; 71.5. Cronbach’s alpha of five dimensions was 0.85. EQ-5D-5L has a good convergent validity with VAS (0.73. It discriminated patients at different HIV/AIDS stages, duration of ART, and CD4 cell count. Predictors of poorer HRQOL included being female, lower education level, unemployment, alcohol and drug use, CD4 Conclusion The EQ-5D-5L has good measurement properties in HIV/AIDS patients and holds potentials for monitoring ART outcomes. Integration of HRQOL measurement using EQ-5D-5L in HIV/AIDS clinical practice could be helpful for economic evaluation of HIV/AIDS interventions.

  18. Experiment data report for semiscale Mod-1 test S-06-4 (LOFT counterpart test)

    International Nuclear Information System (INIS)

    Gillins, R.L.; Sackett, K.E.; Coppin, C.E.

    1977-12-01

    Recorded test data are presented for Test S-06-4 of the Semiscale Mod-1 LOFT counterpart test series. These tests are among several Semiscale Mod-1 experiments conducted to investigate the thermal and hydraulic phenomena accompanying a hypothesized loss-of-coolant accident in a pressurized water reactor (PWR) system. Test S-06-4 was conducted from initial conditions of 15,653 kPa and 564 K to investigate the response of the Semiscale Mod-1 system to a depressurization and reflood transient following a simulated double-ended offset shear of the broken loop cold leg piping. During the test, cooling water was injected into the cold leg of the intact loop to simulate emergency core coolant injection in a PWR. The heater rods in the electrically heated core were operated at an axial peak power density which was 100 percent of the maximum peak power density

  19. Experiment data report for semiscale Mod-1 test S-06-1 (LOFT counterpart test)

    International Nuclear Information System (INIS)

    Collins, B.L.; Patton, M.L. Jr.; Sackett, K.E.

    1977-07-01

    Recorded test data are presented for Test S-06-1 of the Semiscale Mod-1 LOFT counterpart test series. These tests are among several Semiscale Mod-1 experiments conducted to investigate the thermal and hydraulic phenomena accompanying an hypothesized loss-of-coolant accident in a pressurized water reactor (PWR) system. Test S-06-1 was conducted from initial conditions of 15 568 kPa and 564 K to investigate the response of the Semiscale Mod-1 system to a depressurization and reflood transient following a simulated double-ended offset shear of the broken loop cold leg piping. During the test, cooling water was injected into the cold leg of the intact loop to simulate emergency core coolant injection in a PWR. The heater rods in the electrically heated core were operated at an axial peak power density which was 30% of the maximum peak power density

  20. Reactor noise diagnostics based on multivariate autoregressive modeling: Application to LOFT [Loss-of-Fluid-Test] reactor process noise

    International Nuclear Information System (INIS)

    Gloeckler, O.; Upadhyaya, B.R.

    1987-01-01

    Multivariate noise analysis of power reactor operating signals is useful for plant diagnostics, for isolating process and sensor anomalies, and for automated plant monitoring. In order to develop a reliable procedure, the previously established techniques for empirical modeling of fluctuation signals in power reactors have been improved. Application of the complete algorithm to operational data from the Loss-of-Fluid-Test (LOFT) Reactor showed that earlier conjectures (based on physical modeling) regarding the perturbation sources in a Pressurized Water Reactor (PWR) affecting coolant temperature and neutron power fluctuations can be systematically explained. This advanced methodology has important implication regarding plant diagnostics, and system or sensor anomaly isolation. 6 refs., 24 figs

  1. Platinum complexes of 5,6-Dihydroacenaphtho[5,6-cd]-1,2-dichalcogenoles

    OpenAIRE

    Benson, Callum G. M.; Schofield, Catherine M.; Randall, Rebecca A. M.; Wakefield, Lucy; Knight, Fergus R.; Slawin, Alexandra M. Z.; Woollins, J. Derek

    2013-01-01

    Six bis(phosphane) platinum complexes bearing dichalcogen acenaphthene ligands have been prepared by metathesis from cis-[PtCl2(PR3)(2)] (R-3 = Ph-3, Ph2Me, PhMe2) and the dilithium salts of the parent 5,6-dihydroacenaphtho[5,6-cd]-1,2-dichalcogenoles (AcenapE(2), L1 E = S, L2 E = Se). For their synthesis, the appropriate disulfide or diselenide species was treated with super hydride [LiBEt3H] to afford the dilithium salt by in situ reduction of the AcenapE(2) E-E bond. Further reaction, by m...

  2. trans-(Cl)-[Ru(5,5'-diamide-2,2'-bipyridine)(CO)2 Cl2 ]: Synthesis, Structure, and Photocatalytic CO2 Reduction Activity.

    Science.gov (United States)

    Kuramochi, Yusuke; Fukaya, Kyohei; Yoshida, Makoto; Ishida, Hitoshi

    2015-07-06

    A series of trans-(Cl)-[Ru(L)(CO)2 Cl2 ]-type complexes, in which the ligands L are 2,2'-bipyridyl derivatives with amide groups at the 5,5'-positions, are synthesized. The C-connected amide group bound to the bipyridyl ligand through the carbonyl carbon atom is twisted with respect to the bipyridyl plane, whereas the N-connected amide group is in the plane. DFT calculations reveal that the twisted structure of the C-connected amide group raises the level of the LUMO, which results in a negative shift of the first reduction potential (Ep ) of the ruthenium complex. The catalytic abilities for CO2 reduction are evaluated in photoreactions (λ>400 nm) with the ruthenium complexes (the catalyst), [Ru(bpy)3 ](2+) (bpy=2,2'-bipyridine; the photosensitizer), and 1-benzyl-1,4-dihydronicotinamide (the electron donor) in CO2 -saturated N,N-dimethylacetamide/water. The logarithm of the turnover frequency increases by shifting Ep a negative value until it reaches the reduction potential of the photosensitizer. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. The new Nissan FF Hybrid + new 4-cylinder 2.5L supercharged engine. A new approach to powersource evolution

    Energy Technology Data Exchange (ETDEWEB)

    Minakawa, Shunichi; Onoyama, Taiichi; Ishizuka, Takashi [Nissan Motor Co., Ltd., Kanagawa (Japan)

    2013-08-01

    In 2013, Nissan is going to launch a technology combination of 1-Motor 2-Clutch Parallel Hybrid system and 4-cylinder 2.5L supercharged engine for Infiniti QX60 HYBRID and new Nissan Pathfinder HYBRID. This Hybrid system has been developed to have three key advantages: (1) Significant improvement in fuel economy even when Highway driving, (2) Better response and more direct feeling, (3) Lightweight system and low-cost. To achieve these objectives, Nissan fitted a 1-Motor 2-Clutch Parallel system onto a new generation CVT without Torque Converter, combined with downsized 4 cylinder 2.5L supercharged Engine, which provides better fuel economy and V6 3.5-liter class driving performance. Nissan has developed the following proprietary technologies to realize the development of this Hybrid system: - High output and compact Li-ion battery, - Innovative high speed and precise motor and clutch control. Additionally, new technologies are added to both Engine and CVT. Engine: New 4-lobes Roots supercharger with precise electrical boost control. CVT: Chain driven, high efficiency and low friction with wide cover ratio. In this paper Nissan will introduce an outline of the system, key breakthrough technologies and how low fuel economy comparable with the compact car class, has been achieved and demonstrated. (orig.)

  4. Implementation of non-condensable gases condensation suppression model into the WCOBRA/TRAC-TF2 LOCA safety evaluation code

    Energy Technology Data Exchange (ETDEWEB)

    Liao, J.; Cao, L.; Ohkawa, K.; Frepoli, C. [LOCA Integrated Services I, Westinghouse Electric Company, 1000 Westinghouse Drive, Cranberry Township, PA 16066 (United States)

    2012-07-01

    The non-condensable gases condensation suppression model is important for a realistic LOCA safety analysis code. A condensation suppression model for direct contact condensation was previously developed by Westinghouse using first principles. The model is believed to be an accurate description of the direct contact condensation process in the presence of non-condensable gases. The Westinghouse condensation suppression model is further revised by applying a more physical model. The revised condensation suppression model is thus implemented into the WCOBRA/TRAC-TF2 LOCA safety evaluation code for both 3-D module (COBRA-TF) and 1-D module (TRAC-PF1). Parametric study using the revised Westinghouse condensation suppression model is conducted. Additionally, the performance of non-condensable gases condensation suppression model is examined in the ACHILLES (ISP-25) separate effects test and LOFT L2-5 (ISP-13) integral effects test. (authors)

  5. Anchoring Vignettes in EQ-5D-5L Questionnaire: Validation of a New Instrument.

    Science.gov (United States)

    Azzolina, Danila; Minto, Clara; Boschetto, Stefania; Martinato, Matteo; Bauce, Barbara; Iliceto, Sabino; Gregori, Dario

    2017-01-01

    Health Related Quality of Life (HRQoL) is an indicator of patient's physical, psychological and social life. HRQoL is influenced by experience, beliefs, perceptions and expectations, and measures subjective perspective of the patient himself. EQ-5D-5L and SF-12 questionnaires are validated instruments useful to measure HRQoL, increasingly administered in electronic formats. The main purpose is to evaluate the feasibility of anchoring vignettes for the EQ-5D-5L questionnaire, with the aim to improve intergroup comparability of responses among different subjects. A comparison with SF-12 questionnaire is carried out. This is a cross-sectional study conducted at the ambulatories of cardiology of the University Hospital of Padova, in Italy. Thirty-eight subjects with a diagnosis of cardiovascular disease or at risk of cardiovascular disease were enrolled. A factorial analysis has been performed to assess the convergent validity of EQ-5D-5L questionnaire compared to Sf-12. Moreover, a compound Hierarchical Ordered Probit (Chopit) model has been estimated to evaluate if the questionnaire form affects the subjective evaluation process in order to compare EQ-5D-5L with and without vignettes. Correlation and factor analysis demonstrate that EQ_5D questionnaire is coherent with SF-12 in paper format. Chopit model estimation shows that questionnaire format does not affect the subjective question interpretation. Moreover, in a parametric model including vignettes, education attainment, disease severity, and gender are predictors of HRQoL status. The EQ-5D including vignettes in electronic format seems to be a valid tool to measure HRQoL as compared to EQ-5D without vignettes in paper format and to SF-12 questionnaire.

  6. Electric dipole moments of He atoms excited to the 1s5l (l  ⩾ 2) states by He+-ion impact at intermediate energies

    International Nuclear Information System (INIS)

    Baszanowska, E; Drozdowski, R; Kamiński, P; Von Oppen, G

    2014-01-01

    The post-collisional 1s5l (l ⩾ 2) states of He atoms after He + -ion impact (10 keV–28 keV) have been investigated using anticrossing spectroscopy. In particular, the intensity of the spectral line λ(1s5l 3 D-1s2p 3 P) = 402.6 nm emitted by the impact-excited He atoms was measured as a function of an axial electric field (which varied from −30 kV cm −1 to +30 kV cm −1 ). By fitting the theoretical intensity functions to the measured ones, the post-collisional states of the atoms and their electric dipole moments were determined. The results indicate that for projectile energies below 20 keV, the electric dipole moments are small; however, for energies above 20 keV, mainly the parabolic Stark states with maximal electric dipole moments are excited. We conclude that in the upper section of the energy range investigated here, the Paul-trap promotion is the dominant excitation mechanism for He + –He collisions. (paper)

  7. The new 2.5L L4 gasoline engine for LEXUS IS300h. The renewed engine series for FR hybrid vehicles

    Energy Technology Data Exchange (ETDEWEB)

    Ishiguro, Fumihisa; Mashiki, Zenichiro; Yamanari, Kenji [Toyota Motor Corporation, Aichi (Japan)

    2013-08-01

    To ensure future sustainable mobility, vehicles have to face crucial problems such as energy security, global warming and air quality. To address these problems, TOYOTA introduced in 1997 the TOYOTA Hybrid System (THS) and has continuously improved its technology, globally contributing to the environmental improvement by its expansion into many markets in the world. TOYOTA presented the renewed 4 Cylinder Engine Series for FF vehicles at this symposium last year. This year, Toyota presents the result of its continuous development for FR Hybrid Vehicles, especially for D and E segment vehicles. Toyota not only improved thermal efficiency and reduced CO{sub 2} but also achieved high power output. In 2012, TOYOTA launched 3.5L V6 engine for LEXUS-GS (2GR-FXE engine), as first engine of this new engine series. In 2013, TOYOTA will launch 2.5L-L4 engine for LEXUS-IS (2AR-FSE engine), as the leading engine of this series. Toyota developed these new engines, aiming at leadership with regard to fuel economy, clean exhaust gas and high reliability. Toyota carried out modifications, taking into account usage of Hybrid System. With targeting highest levels of environmental performance, Toyota improved the vehicle driving performances like acceleration response by the combination of enhanced engine power output and newly developed Hybrid System. One of the biggest feature of this series is new generation D-4S system, including two injectors (for Direct injection and Port injection) for each cylinder, high fuel pressure (greater than 18MPa) and high fuel flow rate. The new generation D-4S system helps achieving high performance and good thermal efficiency. Furthermore, Toyota added on Cooled EGR system to 2AR-FSE engine, contributing to lower the fuel consumption, especially in highway driving. The very low friction technology and the well proven Atkinson Cycle are also key features of this engine series. Thanks to the above features, the new engine series achieves high

  8. Aterosclerosis subclínica y perfil metabólico en mujeres asintomáticas de edad media, con TSH ≥ 2,5 uUI/mL

    Directory of Open Access Journals (Sweden)

    Rosa M. Pando-Álvarez

    2012-04-01

    Full Text Available El hipotiroidismo subclínico puede contribuir al desarrollo de patologías cardiovasculares y se ha demostrado que a partir de niveles de TSH >2,5uUI/mL se desarrolla disfunción endotelial. Objetivos: Determinar el grosor de la íntima media carotídea (GIMC, la presencia de placas en carótidas y el perfil metabólico en mujeres de edad media asintomáticas con TSH ≥2,5 uUI/mL y compararlas con aquellas con niveles <2,5 uUI/mL. Diseño: Estudio transversal y analítico. Lugar: Hospital Nacional Dos de Mayo e Instituto de Investigaciones Clínicas, Universidad Nacional Mayor de San Marcos, Lima, Perú. Participantes: Mujeres sin historia de enfermedad tiroidea, cardiovascular o diabetes. Intervenciones: En 60 mujeres sin historia de enfermedad tiroidea, cardiovascular o diabetes, con edad promedio de 53,8 ± 5,8 años, se determinó la tirotropina (TSH, colesterol total (CT, colesterol de densidad alta (HDL, triglicéridos (Tg, glucosa (G basal y a los 120 minutos (TTGO, insulina basal (Ins-B; se calculó las fracciones de colesterol de densidad baja (LDL, el nivel de insulinorresistencia (HOMA-IR, la presión arterial, perímetro de cintura abdominal, el índice de masa corporal (IMC y el GIMC mediante ecoDoppler. Principales medidas de resultados: TSH ≥2,5 uUI/mL, perfil metabólico y su relación con GIMC. Resultados: El 38,3% presentó TSH ≥2,5 y 61,7% TSH <2,5 uUI/mL. El 56% de mujeres con TSH ≥2,5 y 65% con TSH <2,5 uUI/ml fueron hipertensas, sin diferencia estadística. El perfil lipídico, G basal, Ins-B e índice HOMA-IR fueron semejantes en ambos grupos. Se observó niveles significativamente más altos del IMC, G a los 120 minutos, el GIMC en carótida izquierda y el mayor entre ambas carótidas en las mujeres con TSH ≥2,5 uUI/mL (p=0,03, p=0,01, p=0,008 y p=0,02, respectivamente. La presencia de placas en la carótida izquierda y en al menos una de las carótidas fue significativamente más frecuente entre aquellas con

  9. Hippocampal Gene Expression of Deiodinases 2 and 3 and Effects of 3,5-Diiodo-L-Thyronine T2 in Mouse Depression Paradigms

    Science.gov (United States)

    Markova, Natalyia; Chernopiatko, Anton; Schroeter, Careen A.; Malin, Dmitry; Kubatiev, Aslan; Bachurin, Sergey; Costa-Nunes, João; Steinbusch, Harry M. W.; Strekalova, Tatyana

    2013-01-01

    Central thyroid hormone signaling is important in brain function/dysfunction, including affective disorders and depression. In contrast to 3,3′,5-triiodo-L-thyronine (T3), the role of 3,5-diiodo-L-thyronine (T2), which until recently was considered an inactive metabolite of T3, has not been studied in these pathologies. However, both T3 and T2 stimulate mitochondrial respiration, a factor counteracting the pathogenesis of depressive disorder, but the cellular origins in the CNS, mechanisms, and kinetics of the cellular action for these two hormones are distinct and independent of each other. Here, Illumina and RT PCR assays showed that hippocampal gene expression of deiodinases 2 and 3, enzymes involved in thyroid hormone regulation, is increased in resilience to stress-induced depressive syndrome and after antidepressant treatment in mice that might suggest elevated T2 and T3 turnover in these phenotypes. In a separate experiment, bolus administration of T2 at the doses 750 and 1500 mcg/kg but not 250 mcg/kg in naive mice reduced immobility in a two-day tail suspension test in various settings without changing locomotion or anxiety. This demonstrates an antidepressant-like effect of T2 that could be exploited clinically. In a wider context, the current study suggests important central functions of T2, whose biological role only lately is becoming to be elucidated. PMID:24386638

  10. Hippocampal Gene Expression of Deiodinases 2 and 3 and Effects of 3,5-Diiodo-L-Thyronine T2 in Mouse Depression Paradigms

    Directory of Open Access Journals (Sweden)

    Natalyia Markova

    2013-01-01

    Full Text Available Central thyroid hormone signaling is important in brain function/dysfunction, including affective disorders and depression. In contrast to 3,3′,5-triiodo-L-thyronine (T3, the role of 3,5-diiodo-L-thyronine (T2, which until recently was considered an inactive metabolite of T3, has not been studied in these pathologies. However, both T3 and T2 stimulate mitochondrial respiration, a factor counteracting the pathogenesis of depressive disorder, but the cellular origins in the CNS, mechanisms, and kinetics of the cellular action for these two hormones are distinct and independent of each other. Here, Illumina and RT PCR assays showed that hippocampal gene expression of deiodinases 2 and 3, enzymes involved in thyroid hormone regulation, is increased in resilience to stress-induced depressive syndrome and after antidepressant treatment in mice that might suggest elevated T2 and T3 turnover in these phenotypes. In a separate experiment, bolus administration of T2 at the doses 750 and 1500 mcg/kg but not 250 mcg/kg in naive mice reduced immobility in a two-day tail suspension test in various settings without changing locomotion or anxiety. This demonstrates an antidepressant-like effect of T2 that could be exploited clinically. In a wider context, the current study suggests important central functions of T2, whose biological role only lately is becoming to be elucidated.

  11. Development and implementation of a novel assay for L-2-hydroxyglutarate dehydrogenase (L-2-HGDH) in cell lysates: L-2-HGDH deficiency in 15 patients with L-2-hydroxyglutaric aciduria

    DEFF Research Database (Denmark)

    Kranendijk, M; Salomons, G S; Gibson, K M

    2009-01-01

    L-2-hydroxyglutaric aciduria (L-2-HGA) is a rare inherited autosomal recessive neurometabolic disorder caused by mutations in the gene encoding L-2-hydroxyglutarate dehydrogenase. An assay to evaluate L-2-hydroxyglutarate dehydrogenase (L-2-HGDH) activity in fibroblast, lymphoblast and/or lymphoc...

  12. Effect of 2,4-D Levels and Tropical Maize Genotypes (Zea mays L

    African Journals Online (AJOL)

    LETA TULU BEDADA

    ) selection medium having 5 g/l mannose as selective agent. .... LS macro-and micro-salts, 1.5 mg/l 2,4-D, 1 g/l casein hydrolysate, 68.5 g/l sucrose, 36 g/l ... thiamine HCl, 2 mg/l glycine†; †Glycine is an amino acid but it was included in vitamin.

  13. Two Zinc(II) metal-organic frameworks with mixed ligands of 5-amino-tetrazolate and l,2,4,5-benzenetetracarboxylate: Synthesis, structural diversity and photoluminescent properties

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Xiao-Bing [Department of Chemistry, Shaoguan University, Shaoguan, Guangdong 512005 (China); Lu, Wen-Guan, E-mail: lwg@sgu.edu.cn [Department of Chemistry, Shaoguan University, Shaoguan, Guangdong 512005 (China); Zhong, Di-Chang [School of Chemistry and Chemical Engineering, Gannan Normal University, Ganzhou 341000 (China)

    2017-06-15

    The reactions of Zn(NO{sub 3}){sub 2}·6H{sub 2}O with mixed ligands of 5-amino-tetrazole (Hatz) and l,2,4,5-benzenetetracarboxylic acid (H{sub 4}btec) under hydro(solvo)thermal conditions, gave two three-dimensional (3D) porous metal-organic frameworks (MOFs) of ([Zn{sub 3}(atz){sub 2}(btec)(DMF){sub 2}]·DMF·2H{sub 2}O){sub n} (1) and [Zn{sub 2}(Hprz)(atz)(btec)(H{sub 2}O)]{sub n} (2) in the absence and presence of piperazine (prz), respectively. 1 and 2 were characterized by infrared spectra (IR), elemental analyses (EA) and single-crystal/powder X-ray diffraction. In 1, the adjacent 1D [Zn{sub 3}(btec)]{sub n}{sup 2n+} chains are linked together by atz{sup −} ligands to form a 3D porous MOF with 1D tetragonal channels filled with coordinated and guest DMF, and lattice water molecules. In 2, the adjacent 2D [Zn{sub 2}(btec)]{sub n} wavelike sheets are pillared through atz{sup −} ligands to generate a 3D layered-pillared porous MOF with 1D open channels, which are occupied by coordinated Hprz{sup +} cations and coordinated water molecules. Additionally, thermal stabilities and photoluminescent properties of both compounds in the solid-state at room temperature have been investigated and discussed in detail. - Graphical abstract: Two new MOFs constructed from Zn(II) salts with mixed ligands of 5-amino-tetrazole and l,2,4,5-benzenetetracarboxylic acid were synthesized under different reaction conditions. Structural diversities indicate that the reaction solvent system or the presence of organic base play crucial roles in modulating structures of these compounds. And more, their thermal stability and luminescence are also discussed. - Highlights: • Two new Zn(II) MOFs based on mixed ligands were synthesized. • The two Zn(II) MOFs exhibit different structural motifs. • The two Zn(II) MOFs are photoluminscent in the solid state at room temperature.

  14. L-2-Hydroxyglutaric aciduria: MRI in seven cases

    Energy Technology Data Exchange (ETDEWEB)

    D`Incerti, L.; Farina, L.; Savoiardo, M. [Dept. of Neuroradiology, Istituto Nazionale Neurologico ``C. Besta``, Milan (Italy); Moroni, I.; Uziel, G. [Dept. of Child Neurology, Istituto Nazionale Neurologico ``C. Besta``, Milan (Italy)

    1998-11-01

    The MRI findings in 7 patients with L-2-Hydroxyglutaric aciduria (L-2-OHG aciduria) are described and compared with previous neuroradiological reports and the only three published pathological cases. Signal abnormalities involved peripheral subcortical white matter, basal ganglia and dentate nuclei. Cerebellar atrophy was present. Although similar appearances may be seen in other metabolic disorders, the distribution of signal abnormalities in L-2-OHG aciduria is highly characteristic and may suggest the correct diagnosis. (orig.) With 5 figs., 2 tabs., 24 refs.

  15. Crystal structure of l-leucyl-l-isoleucine 2,2,2-trifluoroethanol monosolvate

    Directory of Open Access Journals (Sweden)

    Carl Henrik Görbitz

    2016-05-01

    Full Text Available Hydrophobic dipeptides with either l-Leu or l-Phe constitute a rather heterogeneous group of crystal structures. Some form materials with large water-filled channels, but there is also a pronounced tendency to incorporate organic solvent molecules, which then act as acceptors for one of the three H atoms of the charged N-terminal amino group. l-Leu-l-Ile has uniquely been obtained as two distinct hydrates, but has so far failed to co-crystallize with a simple alcohol. The present structure of C12H24N2O3·CF3CH2OH, which crystallizes with two dipeptide and two solvent molecules in the asymmetric unit, demonstrates that when 2,2,2-trifluoroethanol is used as a solvent, its high capacity as a hydrogen-bond donor leads to formation of an alcohol solvate.

  16. Purification and characterization of xylitol dehydrogenase with l-arabitol dehydrogenase activity from the newly isolated pentose-fermenting yeast Meyerozyma caribbica 5XY2.

    Science.gov (United States)

    Sukpipat, Wiphat; Komeda, Hidenobu; Prasertsan, Poonsuk; Asano, Yasuhisa

    2017-01-01

    Meyerozyma caribbica strain 5XY2, which was isolated from an alcohol fermentation starter in Thailand, was found to catabolize l-arabinose as well as d-glucose and d-xylose. The highest production amounts of ethanol from d-glucose, xylitol from d-xylose, and l-arabitol from l-arabinose were 0.45 g/g d-glucose, 0.60 g/g d-xylose, and 0.61 g/g l-arabinose with 21.7 g/L ethanol, 20.2 g/L xylitol, and 30.3 g/l l-arabitol, respectively. The enzyme with l-arabitol dehydrogenase (LAD) activity was purified from the strain and found to exhibit broad specificity to polyols, such as xylitol, d-sorbitol, ribitol, and l-arabitol. Xylitol was the preferred substrate with K m =16.1 mM and k cat /K m =67.0 min -1 mM -1 , while l-arabitol was also a substrate for the enzyme with K m =31.1 mM and k cat /K m =6.5 min -1  mM -1 . Therefore, this enzyme from M. caribbica was named xylitol dehydrogenase (McXDH). McXDH had an optimum temperature and pH at 40°C and 9.5, respectively. The McXDH gene included a coding sequence of 1086 bp encoding a putative 362 amino acid protein of 39 kDa with an apparent homopentamer structure. Native McXDH and recombinant McXDH exhibited relative activities toward l-arabitol of approximately 20% that toward xylitol, suggesting the applicability of this enzyme with the functions of XDH and LAD to the development of pentose-fermenting Saccharomyces cerevisiae. Copyright © 2016 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  17. Utilisation de l'essai comete et du biomarqueur gamma-H2AX pour detecter les dommages induits a l'ADN cellulaire par le 5-bromodeoxyuridine post-irradiation

    Science.gov (United States)

    La Madeleine, Carole

    Ce memoire est presente a la Faculte de medecine et des sciences de la sante de l'Universite de Sherbrooke en vue de l'obtention du grade de maitre es sciences (M.Sc.) en radiobiologie (2009). Un jury a revise les informations contenues dans ce memoire. Il etait compose de professeurs de la Faculte de medecine et des sciences de la sante soit : Darel Hunting PhD, directeur de recherche (departement de medecine nucleaire et radiobiologie), Leon Sanche PhD, directeur de recherche (departement de medecine nucleaire et radiobiologie), Richard Wagner PhD, membre du programme (departement de medecine nucleaire et radiobiologie) et Guylain Boissonneault PhD, membre exterieur au programme (departement de biochimie). Le 5-bromodeoxyuridine (BrdU), un analogue halogene de la thymidine reconnu depuis les annees 60 comme etant un excellent radiosensibilisateur. L'hypothese la plus repandue au sujet de l'effet radio sensibilisant du BrdU est qu'il augmente le nombre de cassures simple et double brin lorsqu'il est incorpore dans l'ADN de la cellule et expose aux radiations ionisantes. Toutefois, de nouvelles recherches semblent remettre en question les observations precedentes. Ces dernieres etudes ont confirme que le BrdU est un bon radiosensibilisateur, car il augmente les dommages radio-induits dans l'ADN. Mais, c'est en etant incorpore dans une region simple brin que le BrdU radiosensibilise l'ADN. Ces recherches ont egalement revele pour la premiere fois un nouveau type de dommages produits lors de l'irradiation de l'ADN contenant du BrdU : les dimeres interbrins. Le but de ces travaux de recherche est de determiner si la presence de bromodeoxyuridine dans l'ADN augmente l'induction de bris simple et / ou double brin chez les cellules irradiees en utilisant de nouvelles techniques plus sensibles et specifiques que celles utilisees auparavant. Pour ce faire, les essais cometes et la detection des foci H2AX phosphorylee pourraient permettre d'etablir les effets engendres par

  18. Corrosive effect of oil's accompanying water polluted with H2S over steel (API 5L X-52)

    International Nuclear Information System (INIS)

    Cueli Corugedo, Alexander; Adames Montero, Yosmari; Rivera Beltran, Yischy; Davis Harriet, Juan

    2013-01-01

    The corrosion from the steel to the carbon in the sale oil pipage conduction, is a serious problem, due to the material and economical looses they cause, damaging even in some cases the productive field. The purpose of this study is to determine the aggressiveness of the oil's water layer, polluted with H 2 S ( g) , over the steel of pipelines' construction (API 5L X-52), taking into account the temperature variations which take place during the transportation of the oil, using the electrochemical techniques of polarisation resistance (LPR) and electrochemical noise. It is pretended to determine the velocity of steel corrosion in the oil's water layer polluted with H 2 S through electrochemical techniques. It was shown that the temperature increases and the concentration of H 2 S to 500 ppm in the oil's accompanying water emphasizes the corrosion phenomenon experienced by the steel (9, 188 0 mm/year to 70℃).The results of the electrochemical noise spectrums and the values of the localisation ?s index calculated, shown the presence of corrosion on the steel surface (API 5L X-52).This result was complemented through optic Microscopy which permits to corroborate the poor adherence of the sulphur layers deposited on the metal that increase the appearance of events found with the temperature increase and the concentration of H 2 S in the environment studied

  19. External attachment of titanium sheathed thermocouples to zirconium nuclear fuel rods for the LOFT reactor

    International Nuclear Information System (INIS)

    Welty, R.K.

    1980-01-01

    The Exxon Nuclear Company, Inc., acting as a Subcontractor to EG and G Idaho Inc., Idaho National Engineering Laboratory, Idaho Falls, Idaho, has developed a welding process to attach titanium sheathed thermocouples to the outside of the zircaloy clad fuel rods. The fuel rods and thermocouples are used to test simulated loss-of-coolant accident (LOCA) conditions in a pressurized water reactor (LOFT Reactor, Idaho National Laboratory). A laser beam was selected as the optimum welding process because of the extremely high energy input per unit volume that can be achieved allowing local fusion of a small area irrespective of the difference in material thickness to be joined. A commercial pulsed laser and energy control system was installed along with specialized welding fixtures. Laser room facility requirements and tolerances were established. Performance qualifications, and detailed welding procedures were also developed. Product performance tests were conducted to assure that engineering design requirements could be met on a production basis

  20. Investigating Trojan Asteroids at the L4/L5 Sun-Earth Lagrange Points

    Science.gov (United States)

    John, K. K.; Graham, L. D.; Abell, P. A.

    2015-01-01

    Investigations of Earth's Trojan asteroids will have benefits for science, exploration, and resource utilization. By sending a small spacecraft to the Sun-Earth L4 or L5 Lagrange points to investigate near-Earth objects, Earth's Trojan population can be better understood. This could lead to future missions for larger precursor spacecraft as well as human missions. The presence of objects in the Sun-Earth L4 and L5 Lagrange points has long been suspected, and in 2010 NASA's Wide-field Infrared Survey Explorer (WISE) detected a 300 m object. To investigate these Earth Trojan asteroid objects, it is both essential and feasible to send spacecraft to these regions. By exploring a wide field area, a small spacecraft equipped with an IR camera could hunt for Trojan asteroids and other Earth co-orbiting objects at the L4 or L5 Lagrange points in the near-term. By surveying the region, a zeroth-order approximation of the number of objects could be obtained with some rough constraints on their diameters, which may lead to the identification of potential candidates for further study. This would serve as a precursor for additional future robotic and human exploration targets. Depending on the inclination of these potential objects, they could be used as proving areas for future missions in the sense that the delta-V's to get to these targets are relatively low as compared to other rendezvous missions. They can serve as platforms for extended operations in deep space while interacting with a natural object in microgravity. Theoretically, such low inclination Earth Trojan asteroids exist. By sending a spacecraft to L4 or L5, these likely and potentially accessible targets could be identified.

  1. Detailed analysis of RNA-protein interactions within the bacterial ribosomal protein L5/5S rRNA complex.

    Science.gov (United States)

    Perederina, Anna; Nevskaya, Natalia; Nikonov, Oleg; Nikulin, Alexei; Dumas, Philippe; Yao, Min; Tanaka, Isao; Garber, Maria; Gongadze, George; Nikonov, Stanislav

    2002-12-01

    The crystal structure of ribosomal protein L5 from Thermus thermophilus complexed with a 34-nt fragment comprising helix III and loop C of Escherichia coli 5S rRNA has been determined at 2.5 A resolution. The protein specifically interacts with the bulged nucleotides at the top of loop C of 5S rRNA. The rRNA and protein contact surfaces are strongly stabilized by intramolecular interactions. Charged and polar atoms forming the network of conserved intermolecular hydrogen bonds are located in two narrow planar parallel layers belonging to the protein and rRNA, respectively. The regions, including these atoms conserved in Bacteria and Archaea, can be considered an RNA-protein recognition module. Comparison of the T. thermophilus L5 structure in the RNA-bound form with the isolated Bacillus stearothermophilus L5 structure shows that the RNA-recognition module on the protein surface does not undergo significant changes upon RNA binding. In the crystal of the complex, the protein interacts with another RNA molecule in the asymmetric unit through the beta-sheet concave surface. This protein/RNA interface simulates the interaction of L5 with 23S rRNA observed in the Haloarcula marismortui 50S ribosomal subunit.

  2. Relative bioavailability of a newly developed 5-mg levomethadone hydrochloride IR tablet (L-Polamidon® 5 mg tablets) in comparison with the 5-mg levomethadone hydrochloride oral solution (L-Polamidon® solution for substitution) as reference product.

    Science.gov (United States)

    Blume, Henning H; Wedemeyer, Ralf-Steven; Donath, Frank; Roscher, Katrin; Elvert, Gerd; Wagner, Daniel; Bley, Oliver; Vuia, Alexander; Todorova-Sanjari, Marina; Villalobos, Ramon; Schug, Barbara

    2015-04-01

    To establish the relative bioavailability (rBA) between two p.o. 5-mg levomethadone hydrochloride formulations, i.e., L-Polamidon® 5 mg tablets (test) vs. L-Polamidon® solution for substitution (reference). To assess the safety and tolerability of both formulations. A total of 33 healthy male subjects, aged 29 ± 6 years (BMI: 23.9 ± 2.5 kg/m2) completed this single center, open-label, randomized, 2-period cross-over study with single dose administrations under fasting conditions and coadministration with naltrexone for safety reasons. Administrations of both investigational products were separated by a washout period of at least 2 weeks, i.e., 13 treatmentfree days. The total dose for each subject was 2 x 5 mg resulting in 10 mg levomethadone hydrochloride. For pharmacokinetic evaluation, blood samples were withdrawn until 72 hours postdose. A validated non-stereoselective liquid chromatography-tandem mass spectroscopy method (LC-MS/MS) was applied for the determination of levomethadone in plasma. The lower limit of quantitation was 0.100 ng/mL. Adverse events were descriptively analyzed in the study population. The geometric means of the parameters related with the extent of total exposure of levomethadone, i.e., AUC(0-tlast) and AUC(0-∞), were 244.422 ng x h/mL and 332.999 ng x h/mL for test and 246.837 ng x h/mL and 329.467 ng×h/mL for reference, respectively. The geometric means of the peak exposure for levomethadone, i.e., Cmax, were 8.923 ng/mL for test and 8.635 ng/mL for reference. The point estimates (PEs) of the Test/Reference (T/R) adjusted geometric mean ratios of AUC(0-last), AUC(0-∞), and C(max) were 99.20%, 101.42%, and 104.11%, respectively, and all of them showed 90%-confidence intervals (CIs) within the range of 80.00 - 125.00% as suggested by regulatory requirements for bioequivalence assessment In total, 21 subjects experienced 55 AEs during the study, the most frequently reported AE, i.e., headache, accounted for 13 out of the total

  3. Deoxyfluoroketohexoses: 4-deoxy-4-fluoro-D-sorbose and -tagatose and 5-deoxy-5-fluoro-L-sorbose.

    Science.gov (United States)

    Rao, G V; Que, L; Hall, L D; Fondy, T P

    1975-04-01

    4-Deoxy-4-fluoro-alpha-D-sorbose (6) was prepared in crystalline form by the action of potassium hydrogen fluoride on 3,4-anhydro-1,2-O-isopropylidene-beta-D-psicopyranose (3) followed by deacetonation. Under identical conditions, 3,4-anhydro-1,2-O-isopropylidene-beta-D-tagatopyranose (7) underwent epoxide migration to give 4,5-anhydro-1,2-O-isopropylidene-beta-D-fructopyranose (12), which after deacetonation yielded 4-deoxy-4-fluoro-D-tagatose (15) and 5-deoxy-5-fluoro-alpha-L-sorbopyranose (16), the latter as the crystalline, free sugar. The action of glycol-cleavage reagents on the isopropylidene acetals of the deoxyfluoro sugars was consistent with the assigned structures. The structures were established by 13-C n.m.r. studies of the free deoxyfluoro sugars 6 and 16 and of the isopropylidene acetal 13, and by 1-H n.m.r. studies on the acetylated isopropylidene acetals 5 diacetate, 13 diacetate, and 14 diacetate. 5-Deoxy-5-fluoro-L-sorbose (16) was biologically active, producing in mice effects characteristic of deoxyfluorotrioses and of fluoroacetate. 4-Deoxy-4-fluoro-D-tagatose (15) and 4-deoxy-4-fluoro-D-sorbose (6) produced no apparent effects in mice up to a dose of 500mg/kg. The implications of these findings with respect to transport, phosphorylation, and the action of aldolase on ketohexoses are discussed.

  4. STAT5A-mediated NOX5-L expression promotes the proliferation and metastasis of breast cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Dho, So Hee [Aging Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806 (Korea, Republic of); Radioisotope Research Division, Department of Research Reactor Utilization, Korea Atomic Energy Research Institute, Daejeon 305-353 (Korea, Republic of); Kim, Ji Young; Lee, Kwang-Pyo; Kwon, Eun-Soo [Aging Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806 (Korea, Republic of); Lim, Jae Cheong [Radioisotope Research Division, Department of Research Reactor Utilization, Korea Atomic Energy Research Institute, Daejeon 305-353 (Korea, Republic of); Kim, Chang-Jin [Department of Pathology, Soonchunhyang Medical Science Research Institute, Chonan 330-090 (Korea, Republic of); Jeong, Dongjun, E-mail: juny1024@sch.ac.kr [Department of Pathology, Soonchunhyang Medical Science Research Institute, Chonan 330-090 (Korea, Republic of); Kwon, Ki-Sun, E-mail: kwonks@kribb.re.kr [Aging Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806 (Korea, Republic of); Department of Functional Genomics, Korea University of Science and Technology (UST), Daejeon 305-333 (Korea, Republic of)

    2017-02-01

    NADPH oxidase (NOX) generates reactive oxygen species (ROS) and has been suggested to mediate cell proliferation in some cancers. Here, we show that an increase in the expression of NOX5 long form (NOX5-L) is critical for tumor progression in breast tumor tissues. Immunostaining of clinical samples indicated that NOX5 was overexpressed in 41.1% of breast ductal carcinoma samples. NOX5-L depletion consistently suppressed cell proliferation, invasion, and migration in vitro. Antibody-mediated neutralization of NOX5-L attenuated tumor progression in a mouse xenograft model. Promoter analysis revealed that NOX5-L expression is regulated by STAT5A in breast cancer cells. Based on our novel findings, we suggest that inhibition of NOX5-L may be a promising therapeutic strategy that exerts anti-cancer effects via the modulation of ROS-mediated cell signaling. - Highlights: • The ROS-generating protein, NOX5-L, determines cellular proliferation and metastasis in subset of breast tumor. • Tumor growth was attenuated by the treatment of anti-NOX5-L antibody in a xenograft model. • NOX5-L expression is transcriptionally regulated by STAT5A in breast cancer cells.

  5. Identification of the polypeptides encoded in the unassigned reading frames 2, 4, 4L, and 5 of human mitochondrial DNA

    International Nuclear Information System (INIS)

    Mariottini, P.; Chomyn, A.; Riley, M.; Cottrell, B.; Doolittle, R.F.; Attardi, G.

    1986-01-01

    In previous work, antibodies prepared against chemically synthesized peptides predicted from the DNA sequence were used to identify the polypeptides encoded in three of the eight unassigned reading frames (URFs) of human mitochondrial DNA (mtDNA). In the present study, this approach has been extended to other human mtDNA URFs. In particular, antibodies directed against the NH 2 -terminal octapeptide of the putative URF2 product specifically precipitated component 11 of the HeLa cell mitochondrial translation products, the reaction being inhibited by the specific peptide. Similarly, antibodies directed against the COOH-terminal nonapeptide of the putative URF4 product reacted specifically with components 4 and 5, and antibodies against a COOH-terminal heptapeptide of the presumptive URF4L product reacted specifically with component 26. Antibodies against the NH 2 -terminal heptapeptide of the putative product of URF5 reacted with component 1, but only to a marginal extent; however, the results of a trypsin fingerprinting analysis of component 1 point strongly to this component as being the authentic product of URF5. The polypeptide assignments to the mtDNA URFs analyzed here are supported by the relative electrophoretic mobilities of proteins 11, 4-5, 26, and 1, which are those expected for the molecular weights predicted from the DNA sequence for the products of URF2, URF4, URF4L, and URF5, respectively. With the present assignment, seven of the eight human mtDNA URFs have been shown to be expressed in HeLa cells

  6. Health-related quality of life measured using the EQ-5D-5L: South Australian population norms.

    Science.gov (United States)

    McCaffrey, Nikki; Kaambwa, Billingsley; Currow, David C; Ratcliffe, Julie

    2016-09-20

    Although a five level version of the widely-used EuroQol 5 dimensions (EQ-5D) instrument has been developed, population norms are not yet available for Australia to inform the future valuation of health in economic evaluations. The aim of this study was to estimate HrQOL normative values for the EQ-5D-5L preference-based measure in a large, randomly selected, community sample in South Australia. The EQ-5D-5L instrument was included in the 2013 South Australian Health Omnibus Survey, an interviewer-administered, face-to-face, cross-sectional survey. Respondents rated their level of impairment across dimensions (mobility, self-care, usual activities, pain/discomfort, and anxiety/depression) and global health rating on a visual analogue scale (EQ-VAS). Utility scores were derived using the newly-developed UK general population-based algorithm and relationships between utility and EQ-VAS scores and socio-demographic factors were also explored using multivariate regression analyses. Ultimately, 2,908 adults participated in the survey (63.4 % participation rate). The mean utility and EQ-VAS scores were 0.91 (95 CI 0.90, 0.91) and 78.55 (95 % CI 77.95, 79.15), respectively. Almost half of respondents reported no problems across all dimensions (42.8 %), whereas only 7.2 % rated their health >90 on the EQ-VAS (100 = the best health you can imagine). Younger age, male gender, longer duration of education, higher annual household income, employment and marriage/de facto relationships were all independent, statistically significant predictors of better health status (p measured with the EQ-VAS. Only age and employment status were associated with higher utility scores, indicating fundamental differences between these measures of health status. This is the first Australian study to apply the EQ-5D-5L in a large, community sample. Overall, findings are consistent with EQ-5D-5L utility and VAS scores reported for other countries and indicate that the majority of South

  7. Intramolecular interaction influences binding of the Flax L5 and L6 resistance proteins to their AvrL567 ligands.

    Directory of Open Access Journals (Sweden)

    Michael Ravensdale

    Full Text Available L locus resistance (R proteins are nucleotide binding (NB-ARC leucine-rich repeat (LRR proteins from flax (Linum usitatissimum that provide race-specific resistance to the causal agent of flax rust disease, Melampsora lini. L5 and L6 are two alleles of the L locus that directly recognize variants of the fungal effector AvrL567. In this study, we have investigated the molecular details of this recognition by site-directed mutagenesis of AvrL567 and construction of chimeric L proteins. Single, double and triple mutations of polymorphic residues in a variety of AvrL567 variants showed additive effects on recognition strength, suggesting that multiple contact points are involved in recognition. Domain-swap experiments between L5 and L6 show that specificity differences are determined by their corresponding LRR regions. Most positively selected amino acid sites occur in the N- and C-terminal LRR units, and polymorphisms in the first seven and last four LRR units contribute to recognition specificity of L5 and L6 respectively. This further confirms that multiple, additive contact points occur between AvrL567 variants and either L5 or L6. However, we also observed that recognition of AvrL567 is affected by co-operative polymorphisms between both adjacent and distant domains of the R protein, including the TIR, ARC and LRR domains, implying that these residues are involved in intramolecular interactions to optimize detection of the pathogen and defense signal activation. We suggest a model where Avr ligand interaction directly competes with intramolecular interactions to cause activation of the R protein.

  8. Analysis of results from a loss-of-offsite-power-initiated ATWS experiment in the LOFT Facility

    International Nuclear Information System (INIS)

    Varacalle, D.J.; Giri, A.M.; Koizumi, Y.; Koske, J.E.

    1983-01-01

    An anticipated transient without scram (ATWS), initiated by loss-of-offsite power, was experimentally simulated in the Loss-of-Fluid Test (LOFT) pressurized water reactor (PWR). Primary system pressure was controlled using a scaled safety relief valve (SRV) representative of those in a commercial PWR, while reactor power was reduced by moderator reactivity feedback in a natural circulation mode. The experiment showed that reactor power decreases more rapidly when the primary pumps are tripped in a loss-of-offsite-power ATWS than in a loss-of-feedwater induced ATWS when the primary pumps are left on. During the experiment, the SRV had sufficient relief capacity to control primary system pressure. Natural circulation was effective in removing core heat at high temperature, pressure, and core power. The system transient response predicted using the RELAPS/MOD1 computer code showed good agreement with the experimental data

  9. Activation of the 2-5OAS/RNase L pathway in CVB1 or HAV/18f infected FRhK-4 cells does not require induction of OAS1 or OAS2 expression

    International Nuclear Information System (INIS)

    Kulka, Michael; Calvo, Mona S.; Ngo, Diana T.; Wales, Samantha Q.; Goswami, Biswendu B.

    2009-01-01

    The latent, constitutively expressed protein RNase L is activated in coxsackievirus and HAV strain 18f infected FRhK-4 cells. Endogenous oligoadenylate synthetase (OAS) from uninfected and virus infected cell extracts synthesizes active forms of the triphosphorylated 2-5A oligomer (the only known activator of RNase L) in vitro and endogenous 2-5A is detected in infected cell extracts. However, only the largest OAS isoform, OAS3, is readily detected throughout the time course of infection. While IFNβ treatment results in an increase in the level of all three OAS isoforms in FRhK-4 cells, IFNβ pretreatment does not affect the temporal onset or enhancement of RNase L activity nor inhibit virus replication. Our results indicate that CVB1 and HAV/18f activate the 2-5OAS/RNase L pathway in FRhK-4 cells during permissive infection through endogenous levels of OAS, but contrary to that reported for some picornaviruses, CVB1 and HAV/18f replication is insensitive to this activated antiviral pathway.

  10. Some discussions on micrometeorology and atmospheric diffusion of classic and radioactive industrial pollutions. 5

    International Nuclear Information System (INIS)

    Veverka, O.; Valenta, V.; Vlachovsky, K.

    1976-01-01

    The formulae are given expressing the atmospheric dispersion of industrial emissions released from the stacks of industrial plants and power plants. Gaussian distribution of the emissions is assumed. The behaviour of the plume is discussed and the concepts of lofting and fumigation are explained. Generalized relations are derived for the calculation of the atmospheric dispersion of gaseous wastes applicable to both high and short stack disposal and to the leakage from buildings, assuming that the source of emissions is of a point type and emissions are released continuously. (L.O.)

  11. Spectrophotometric determination of yttrium with 2-(2-thiazolylazo)-5-dimethylaminophenol

    International Nuclear Information System (INIS)

    Tsurumi, Chikao; Furuya, Keiichi.

    1975-01-01

    Spectrophotometric determination of small amounts of yttrium with 2-(2-thiazolylazo)-5-dimethylaminophenol (TAM) in the presence of zephiramine was investigated. The recommended procedures were as follows; 2.0 ml of water, 7.0 ml of TAM-methanolic solution (2x10 -4 mol/l) and 2.0 ml of zephiramine-aqueous solution (1x10 -2 mol/l) were added to a solution containing less than 35 μg of yttrium and its pH was adjusted to 8.0 with 0.1 mol/l ammonium chloride-0.1 mol/l ammonium hydroxide solution. The solution was transferred to a 25 ml volumetric flask and diluted to the mark with water. After 20 minutes, the absorbance at 575 nm against a reagent blank was measured. The color of yttrium-TAM complex is reddish-violet in the presence of zephiramine and is stable for 90 minutes after color development. The yttrium-TAM complex shows an absorption maximum at 575 nm. The absorbance at 575 nm is constant in a pH range from 7.5 to 8.3. The molar extinction coefficient at this wavelength is 7.2x10 4 l.mol -1 .cm -1 . The band obeys Beer's law up to the concentration of 1.4 μg/ml of yttrium. The molar ratio of yttrium to TAM in the complex is 1 : 2. A number of ions interfere with the determination can be masked by the addition of various masking agents and removed in terms of trioctylamine-xylene extraction. Manganese, tantalum, tin(II), citrate and tartrate ions interfere with the determination. (auth.)

  12. Methylated DNMT1 and E2F1 are targeted for proteolysis by L3MBTL3 and CRL4DCAF5 ubiquitin ligase.

    Science.gov (United States)

    Leng, Feng; Yu, Jiekai; Zhang, Chunxiao; Alejo, Salvador; Hoang, Nam; Sun, Hong; Lu, Fei; Zhang, Hui

    2018-04-24

    Many non-histone proteins are lysine methylated and a novel function of this modification is to trigger the proteolysis of methylated proteins. Here, we report that the methylated lysine 142 of DNMT1, a major DNA methyltransferase that preserves epigenetic inheritance of DNA methylation patterns during DNA replication, is demethylated by LSD1. A novel methyl-binding protein, L3MBTL3, binds the K142-methylated DNMT1 and recruits a novel CRL4 DCAF5 ubiquitin ligase to degrade DNMT1. Both LSD1 and PHF20L1 act primarily in S phase to prevent DNMT1 degradation by L3MBTL3-CRL4 DCAF5 . Mouse L3MBTL3/MBT-1 deletion causes accumulation of DNMT1 protein, increased genomic DNA methylation, and late embryonic lethality. DNMT1 contains a consensus methylation motif shared by many non-histone proteins including E2F1, a key transcription factor for S phase. We show that the methylation-dependent E2F1 degradation is also controlled by L3MBTL3-CRL4 DCAF5 . Our studies elucidate for the first time a novel mechanism by which the stability of many methylated non-histone proteins are regulated.

  13. New optically active and thermally stable poly(amide-imide)s containing N,N'-(Bicyclo[2,2,2]oct-7-ene-2,3,5,6-tetracarboxylic)-bis-L-alanine and aromatic diamines: synthesis and characterization

    International Nuclear Information System (INIS)

    Faghihi, Khalil; Absalar, Morteza; Hajibeygi, Mohsen

    2009-01-01

    Five new optically active poly(amide-imide)s (PAIs) 6a-e were prepared by direct polycondensation reaction of the newly synthesized N,N'-(bicyclo[2,2,2]oct-7-ene-2,3,5,6-tetra carboxylic)-bis-L-alanine 4 with various aromatic diamines 5a-e using polar aprotic solvents such as N-methyl-2-pyrrolidone (NMP). In this technique triphenyl phosphite (TPP) and pyridine were used as condensing agents to form poly(amide-imide)s through the N-phosphonium salts of pyridine. All of the polymers were obtained in quantitative yields with inherent viscosities between 0.29-0.46 dL g -1 and were highly soluble in polar aprotic solvents such as N,N-dimethyl acetamide (DMAc), N,N-dimethyl formamide (DMF), dimethyl sulfoxide (DMSO), N-methyl- 2-pyrrolidone (NMP) and solvents such as sulfuric acid. They were fully characterized by means of 1 H NMR, FTIR spectroscopy, elemental analyses, inherent viscosity, solubility test, specific rotation and thermal properties of these polymers were investigated using thermogravimetric analysis techniques (TGA and DTG). (author)

  14. Antimalarial peroxides: the first intramolecular 1,2,4,5-tetraoxane

    Directory of Open Access Journals (Sweden)

    BOGDAN A. SOLAJA

    2002-07-01

    Full Text Available An intramolecular steroidal 1,2,4,5-tetraoxane has been synthesised in six steps starting from methyl 3-oxo-7a,12a-diacetoxy-5b-cholan-24-oate. The synthesised 1,2,4,5-tetraoxane has moderate in vitro antimalarial activity against P. falciparum strains (IC50 (D6 = 0.35 mg/mL; IC50 (W2 = 0.29 mg/mL.

  15. A steady-state mechanism can account for the properties of inositol 2,4,5-trisphosphate-stimulated Ca2+ release from permeabilized L1210 cells.

    Science.gov (United States)

    Loomis-Husselbee, J W; Dawson, A P

    1993-01-01

    We have investigated the effects of sub-maximal Ins(2,4,5)P3 concentrations on the Ca2+ permeability of the residual undischarged Ca2+ stores in electroporated or digitonin-permeabilized L1210 cells by measuring Ca(2+)-efflux rate after addition of the ATPase inhibitor thapsigargin. Low concentrations of Ins(2,4,5)P3, causing rapid discharge of a small proportion of the releasable Ca2+, result in a substantial stimulation of Ca2+ efflux after thapsigargin addition. This indicates firstly that in the absence of thapsigargin there must have been a substantial, counterbalancing, increase in rate of Ca2+ pumping, and secondly that the increased Ca2+ permeability is more consistent with a steady state than with a quantal model of Ca2+ release. Similar increases in passive Ca2+ permeability are produced by addition of concentrations of ionomycin which produce equivalent changes in Ca2+ loading to those produced by Ins(2,4,5)P3, although the time course and initial rate of Ca2+ release are very much slower. In the presence of a Ca(2+)-buffering system, the time course of Ca2+ release by Ins(2,4,5)P3 becomes superimposable on that of ionomycin, indicating that the initial rapid phase of Ins(2,4,5)P3-stimulated Ca2+ is at least partially due to positive feedback from extravesicular Ca2+. PMID:8382056

  16. Diaquabis[5-(2-pyridyltetrazolato-κ2N1,N5]iron(II

    Directory of Open Access Journals (Sweden)

    Min Hu

    2009-04-01

    Full Text Available The title complex, [Fe(C6H4N52(H2O2], was synthesized by the reaction of ferrous sulfate with 5-(2-pyridyl-2H-tetrazole (HL. The FeII atom, located on a crystallographic center of inversion, is coordinated by four N-atom donors from two planar trans-related deprotonated L ligands and two O atoms from two axial water molecules in a distorted octahedral geometry. The FeII mononuclear units are further connected by intermolecular O—H...N and C—H...O hydrogen-bonding interactions, forming a three-dimensional framework.

  17. L1 and L2 Distance Effects in Learning L3 Dutch

    Science.gov (United States)

    Schepens, Job J.; der Slik, Frans; Hout, Roeland

    2016-01-01

    Many people speak more than two languages. How do languages acquired earlier affect the learnability of additional languages? We show that linguistic distances between speakers' first (L1) and second (L2) languages and their third (L3) language play a role. Larger distances from the L1 to the L3 and from the L2 to the L3 correlate with lower…

  18. Acute 5-(2-aminopropyl)benzofuran (5-APB) intoxication and fatality: a case report with postmortem concentrations.

    Science.gov (United States)

    McIntyre, Iain M; Gary, Ray D; Trochta, Amber; Stolberg, Susan; Stabley, Robert

    2015-03-01

    A 20-year-old man, a college student, became unresponsive in front of his girlfriend. He was known to consume alcohol and take an unknown drug at some point while in attendance at a local music festival earlier in the day/evening. Upon arrival of emergency personnel, he was noted to be asystolic and apneic. Despite aggressive medical intervention by emergency personnel and at a local hospital emergency room, he was pronounced deceased within 1.25 h of initial medical attention. Postmortem blood initially screened positive for methamphetamine by ELISA. An alkaline drug screen detected 5-(2-aminopropyl)benzofuran (5-APB) which was subsequently confirmed and quantified by a specific GC-MS SIM analysis following solid-phase extraction. Concentrations were determined in the peripheral blood (2.5 mg/L), central blood (2.9 mg/L), liver (16 mg/kg), vitreous (1.3 mg/L), urine (23 mg/L) and gastric contents (6 mg). No other common amphetamine-like compound was detected, although 5-(2-aminopropyl)-2,3-dihydrobenzofuran (5-APDB) was presumptively identified in both peripheral blood and urine. Alcohol, the only other drug identified, was confirmed at a concentration of 0.02% (w/v). © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  19. Isozyme-specific enzyme inhibitors. 14. 5'(R)-C-[(L-homocystein-S-yl)methyl]adenosine 5'-(beta,gamma-imidotriphosphate), a potent inhibitor of rat methionine adenosyltransferases.

    Science.gov (United States)

    Kappler, F; Vrudhula, V M; Hampton, A

    1987-09-01

    The title compound is a covalent adduct of L-methionine (Met) and beta,gamma-imido-ATP. In its synthesis the N-Boc derivative of 5'(R)-C-(aminomethyl)-N6-benzoyl-5'-O-tosyl-2',3'-O- isopropylidenadenosine was converted by the successive actions of CF3CO2H and HNO2 into the corresponding 5'(R)-C-hydroxymethyl derivative. Treatment of this with disodium L-homocysteinate led to attack of sulfur at C6', apparently via a 5',6'-epoxide, and to total stereoselective inversion at C5' to furnish, after debenzoylation, 5'(R)-C-(L-homocystein-S-ylmethyl)-2',3'-O-isopropylidene ade nosine. The 5' configuration was established by conversion of this into the known 5'(S)-C-methyl-2',3'-O-isopropylidene adenosine with Raney nickel. The alpha-amino acid residue was protected as an N-Boc methyl ester, after which the 5'-hydroxyl was phosphorylated with benzyl phosphate and dicyclohexylcarbodiimide. The phosphoanhydride bond with inorganic imidodiphosphate was then created by established methods. Finally, blocking groups were removed under conditions that gave the desired adduct with no racemization of its L-methionine residue. It was a potent inhibitor [KM(ATP)/Ki = 1080; KM(Met)/Ki = 7.7] of the M-2 (normal tissue) form of rat methionine adenosyltransferase and of the M-T (hepatoma tissue) form [KM(ATP)/Ki = 670; KM(Met)/Ki = 22]. Inhibitions were competitive with respect to ATP or to L-methionine, indicating a dual substrate site mode of binding to the enzyme forms.

  20. Regulation of steroid 5-{alpha} reductase type 2 (Srd5a2) by sterol regulatory element binding proteins and statin

    Energy Technology Data Exchange (ETDEWEB)

    Seo, Young-Kyo [Department of Molecular Biology and Biochemistry, 3244 McGaugh Hall, University of California, UC Irvine, Irvine, CA 92697-3900 (United States); Zhu, Bing [Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0144 (United States); Jeon, Tae-Il [Department of Molecular Biology and Biochemistry, 3244 McGaugh Hall, University of California, UC Irvine, Irvine, CA 92697-3900 (United States); Osborne, Timothy F., E-mail: tfosborn@uci.edu [Department of Molecular Biology and Biochemistry, 3244 McGaugh Hall, University of California, UC Irvine, Irvine, CA 92697-3900 (United States)

    2009-11-01

    In this study, we show that sterol regulatory element binding proteins (SREBPs) regulate expression of Srd5a2, an enzyme that catalyzes the irreversible conversion of testosterone to dihydroxytestosterone in the male reproductive tract and is highly expressed in androgen-sensitive tissues such as the prostate and skin. We show that Srd5a2 is induced in livers and prostate from mice fed a chow diet supplemented with lovastatin plus ezitimibe (L/E), which increases the activity of nuclear SREBP-2. The three fold increase in Srd5a2 mRNA mediated by L/E treatment was accompanied by the induction of SREBP-2 binding to the Srd5a2 promoter detected by a ChIP-chip assay in liver. We identified a SREBP-2 responsive region within the first 300 upstream bases of the mouse Srd5a2 promoter by co-transfection assays which contain a site that bound SREBP-2 in vitro by an EMSA. Srd5a2 protein was also induced in cells over-expressing SREBP-2 in culture. The induction of Srd5a2 through SREBP-2 provides a mechanistic explanation for why even though statin therapy is effective in reducing cholesterol levels in treating hypercholesterolemia it does not compromise androgen production in clinical studies.

  1. Regulation of steroid 5-α reductase type 2 (Srd5a2) by sterol regulatory element binding proteins and statin

    International Nuclear Information System (INIS)

    Seo, Young-Kyo; Zhu, Bing; Jeon, Tae-Il; Osborne, Timothy F.

    2009-01-01

    In this study, we show that sterol regulatory element binding proteins (SREBPs) regulate expression of Srd5a2, an enzyme that catalyzes the irreversible conversion of testosterone to dihydroxytestosterone in the male reproductive tract and is highly expressed in androgen-sensitive tissues such as the prostate and skin. We show that Srd5a2 is induced in livers and prostate from mice fed a chow diet supplemented with lovastatin plus ezitimibe (L/E), which increases the activity of nuclear SREBP-2. The three fold increase in Srd5a2 mRNA mediated by L/E treatment was accompanied by the induction of SREBP-2 binding to the Srd5a2 promoter detected by a ChIP-chip assay in liver. We identified a SREBP-2 responsive region within the first 300 upstream bases of the mouse Srd5a2 promoter by co-transfection assays which contain a site that bound SREBP-2 in vitro by an EMSA. Srd5a2 protein was also induced in cells over-expressing SREBP-2 in culture. The induction of Srd5a2 through SREBP-2 provides a mechanistic explanation for why even though statin therapy is effective in reducing cholesterol levels in treating hypercholesterolemia it does not compromise androgen production in clinical studies.

  2. Crystallization and preliminary X-ray crystallographic analysis of highly thermostable L2 lipase from the newly isolated Bacillus sp. L2

    International Nuclear Information System (INIS)

    Shariff, Fairolniza Mohd; Rahman, Raja Noor Zaliha Raja Abd.; Ali, Mohd Shukuri Mohamad; Chor, Adam Leow Thean; Basri, Mahiran; Salleh, Abu Bakar

    2010-01-01

    Thermostable recombinant L2 lipase from thermophilic Bacillus sp. L2 has been crystallized by using counter-diffusion method and diffracted to 2.7 Å resolution. The crystal belongs to the primitive orthorhombic space group P2 1 2 1 2 1 with unit-cell parameters a = 87.44, b = 94.90, c = 126.46 Å. Purified thermostable recombinant L2 lipase from Bacillus sp. L2 was crystallized by the counter-diffusion method using 20% PEG 6000, 50 mM MES pH 6.5 and 50 mM NaCl as precipitant. X-ray diffraction data were collected to 2.7 Å resolution using an in-house Bruker X8 PROTEUM single-crystal diffractometer system. The crystal belonged to the primitive orthorhombic space group P2 1 2 1 2 1 , with unit-cell parameters a = 87.44, b = 94.90, c = 126.46 Å. The asymmetric unit contained one single molecule of protein, with a Matthews coefficient (V M ) of 2.85 Å 3 Da −1 and a solvent content of 57%

  3. Preparation and characterization of highly L21-ordered full-Heusler alloy Co2FeAl0.5Si0.5 thin films for spintronics device applications

    International Nuclear Information System (INIS)

    Wang Wenhong; Sukegawa, Hiroaki; Shan Rong; Furubayashi, Takao; Inomata, Koichiro

    2008-01-01

    We report the investigation of structure and magnetic properties of full-Heusler alloy Co 2 FeAl 0.5 Si 0.5 (CFAS) thin films grown on MgO-buffered MgO (001) substrates through magnetron sputtering. It was found that single-crystal CFAS thin films with high degree of L2 1 ordering and sufficiently flat surface could be obtained after postdeposition annealing. All the films show a distinct uniaxial magnetic anisotropy with the easy axis of magnetization along the in-plane [110] direction. These results indicate that the use of the MgO buffer for CFAS is a promising approach for achieving a higher tunnel magnetoresistance ratio, and thus for spintronics device applications

  4. Functionalized 2′-amino-α-L-LNA

    DEFF Research Database (Denmark)

    Kumar, T. Santhosh; Madsen, Andreas Stahl; Østergaard, Michael

    2009-01-01

    Chemically modified oligonucleotides are increasingly applied in nucleic acid based therapeutics and diagnostics. LNA (locked nucleic acid) and its diastereomer α-L-LNA are two promising examples thereof that exhibit increased thermal and enzymatic stability. Herein, the synthesis, biophysical......′-functionalities such as 2′-N-acetyl-2′-amino-α-L-LNA (monomer V) had detrimental effects on thermal affinity toward DNA/RNA complements with decreases of as much as -16.5 °C per modification. Extensive thermal DNA selectivity, favorable entropic contributions upon duplex formation, hybridization...

  5. New optically active and thermally stable poly(amide-imide)s containing N,N'-(Bicyclo[2,2,2]oct-7-ene-2,3,5,6-tetracarboxylic)-bis-L-alanine and aromatic diamines: synthesis and characterization

    Energy Technology Data Exchange (ETDEWEB)

    Faghihi, Khalil; Absalar, Morteza; Hajibeygi, Mohsen [Arak University (Iran, Islamic Republic of). Faculty of Science. Organic Polymer Chemistry Research Lab.

    2009-07-01

    Five new optically active poly(amide-imide)s (PAIs) 6a-e were prepared by direct polycondensation reaction of the newly synthesized N,N'-(bicyclo[2,2,2]oct-7-ene-2,3,5,6-tetra carboxylic)-bis-L-alanine 4 with various aromatic diamines 5a-e using polar aprotic solvents such as N-methyl-2-pyrrolidone (NMP). In this technique triphenyl phosphite (TPP) and pyridine were used as condensing agents to form poly(amide-imide)s through the N-phosphonium salts of pyridine. All of the polymers were obtained in quantitative yields with inherent viscosities between 0.29-0.46 dL g{sup -1} and were highly soluble in polar aprotic solvents such as N,N-dimethyl acetamide (DMAc), N,N-dimethyl formamide (DMF), dimethyl sulfoxide (DMSO), N-methyl- 2-pyrrolidone (NMP) and solvents such as sulfuric acid. They were fully characterized by means of {sup 1}H NMR, FTIR spectroscopy, elemental analyses, inherent viscosity, solubility test, specific rotation and thermal properties of these polymers were investigated using thermogravimetric analysis techniques (TGA and DTG). (author)

  6. NEW METABOLITES OF THE DRUG 5-AMINOSALICYLIC ACID .2. N-FORMYL-5-AMINOSALICYLIC ACID

    DEFF Research Database (Denmark)

    Tjornelund, J.; Hansen, S. H.; Cornett, Claus

    1991-01-01

    1. A new metabolite of the drug 5-aminosalicylic acid (5-ASA) has been found in urine from pigs and in plasma of humans. The metabolite has been isolated from pig urine using an XAD-2 column and purified using preparative h.p.l.c. 2. The metabolite has been identified as N-formyl-5-ASA (5-formami...

  7. Measurements of Development in L2 Written Production: The Case of L2 Chinese

    Science.gov (United States)

    Jiang, Wenying

    2013-01-01

    This study investigates measures for second language (L2) writing development. A T-unit, which has been found the most satisfactory unit of analysis for measuring L2 development in English, is extended to measure L2 Chinese writing development through a cross-sectional design in this study. Data were collected from three L2 Chinese learner groups…

  8. Sagittal MR findings of L5 spondylolysis : changes of spinal canal

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hyun Cheol; Choi, Woo Suk; Kim, Eui Jong; Ryu, Kyung Nam; Oh, Joo Hyeong; Kim, Ihn Sub; Yoon, Yup [Kyunghee Univ. Hospital, Seoul (Korea, Republic of)

    1997-07-01

    To evaluate changes in the spinal canal in cases of L5 spondylolysis, as seen on sagittal MR images. We retrospectively analysed the MR findings of 27 patients suffering from L5 spondylolysis without spondylolisthesis and compared them with 100 control subjects. Spondylolysis had been confirmed by conventional radiography. On midsagittal MR images, sagittal canal ratio (SCR) was defined as midsagittal canal diameter at L5 devided by that at L1. We analysed the frequency of posterior epidural fat deposition(posterior epidural fat between the posterior margin of the dural sac and the anterior cortical margin of the spinous process on the midsagittal line), and compared this with the frequency in 100 control subjects. Mean SCR value in 27 patients with L5 spondylolysis(1.22) was significantly greater than 100 control subjects(0.96, p<0.001). Mean SCR value in 17 patients with L5 spondylolysis and posterior epidural fat deposition(1.27) was significantly higher than in nine control subjects with posterior epidural fat deposition(0.97). Posterior epidural fat deposition was more frequently indentified in patients with L5 spondylolysis(63%) than in control subjects(9%). The possibility of L5 spondylolysis is suggested when on midsaggital MR imaging, the anteroposterior diameter of the L5 spinal canal is seen to be widened and posterior epidural fat deposition is noted.

  9. Sagittal MR findings of L5 spondylolysis : changes of spinal canal

    International Nuclear Information System (INIS)

    Kim, Hyun Cheol; Choi, Woo Suk; Kim, Eui Jong; Ryu, Kyung Nam; Oh, Joo Hyeong; Kim, Ihn Sub; Yoon, Yup

    1997-01-01

    To evaluate changes in the spinal canal in cases of L5 spondylolysis, as seen on sagittal MR images. We retrospectively analysed the MR findings of 27 patients suffering from L5 spondylolysis without spondylolisthesis and compared them with 100 control subjects. Spondylolysis had been confirmed by conventional radiography. On midsagittal MR images, sagittal canal ratio (SCR) was defined as midsagittal canal diameter at L5 devided by that at L1. We analysed the frequency of posterior epidural fat deposition(posterior epidural fat between the posterior margin of the dural sac and the anterior cortical margin of the spinous process on the midsagittal line), and compared this with the frequency in 100 control subjects. Mean SCR value in 27 patients with L5 spondylolysis(1.22) was significantly greater than 100 control subjects(0.96, p<0.001). Mean SCR value in 17 patients with L5 spondylolysis and posterior epidural fat deposition(1.27) was significantly higher than in nine control subjects with posterior epidural fat deposition(0.97). Posterior epidural fat deposition was more frequently indentified in patients with L5 spondylolysis(63%) than in control subjects(9%). The possibility of L5 spondylolysis is suggested when on midsaggital MR imaging, the anteroposterior diameter of the L5 spinal canal is seen to be widened and posterior epidural fat deposition is noted

  10. L5 radiculopathy due to sacral stress fracture

    International Nuclear Information System (INIS)

    Aylwin, Anthony; Saifuddin, Asif; Tucker, Stuart

    2003-01-01

    We report the case of a 70-year-old man who presented with a history of left buttock pain with radiation into the left leg in an L5 distribution. MRI of the lumbar spine revealed a left sacral stress fracture with periosteal reaction involving the left L5 nerve root anterior to the sacral ala. With spontaneous healing of the fracture, the patient's symptoms resolved completely. (orig.)

  11. Activation of different pathways of apoptosis by air pollution particulate matter (PM2.5) in human epithelial lung cells (L132) in culture

    International Nuclear Information System (INIS)

    Dagher, Zeina; Garcon, Guillaume; Billet, Sylvain; Gosset, Pierre; Ledoux, Frederic; Courcot, Dominique; Aboukais, Antoine; Shirali, Pirouz

    2006-01-01

    Epidemiological studies have associated the increase of respiratory and cardiovascular mortality and morbidity with high levels of air pollution particulate matter (PM). However, the underlying mechanisms of actions by which PM induce adverse health effects are still unclear. We have recently undertaken an extensive investigation of the adverse health effects of air pollution PM 2.5 , and shown that in vitro short-term exposure to PM 2.5 induced oxidative stress and inflammation in human lung epithelial cells (L132). Hence, it was convenient to complete the physical and chemical characterization of PM and to investigate whether in vitro short-term exposure to PM could be imply in the activation of apoptosis. Accordingly, we found that 92.15% of PM were equal or smaller than 2.5 μm and their specific surface area was 1 m 2 /g. Inorganic (i.e. Fe, Al, Ca, Na, K, Mg, Pb, etc.) and organic (i.e. polycyclic aromatic hydrocarbons) chemicals were found in PM, suggesting that much of them derived from wind-borne dust from the industrial complex and the heavy motor vehicle traffic. In other respects, we showed that PM exposure induced apoptosis by activating not only the tumor necrosis factor-alpha (TNF-α)-induced pathway (i.e. TNF-α secretion, caspase-8 and -3 activation), but also the mitochondrial pathway (i.e. 8-hydroxy-2'-desoxyguanosine formation, cytochrome c release from mitochondria, caspase-9 and -3 activation). Moreover, changes in the transcription rates of p53, bcl-2, and bax genes, on the one hand, and DNA fragmentation, on the other hand, were reported in PM-exposed proliferating L132 cells, revealing the occurrence of apoptotic events. Taken together, these findings suggested that in vitro short-term exposure to PM 2.5 induced apoptosis in L132 cells

  12. N-Acetyl-D- and L-esters of 5'-AMP hydrolyze at different rates

    Science.gov (United States)

    Wickramasinghe, N. S.; Lacey, J. C. Jr; Lacey JC, J. r. (Principal Investigator)

    1993-01-01

    Studies of the properties of aminoacyl derivatives of 5'-AMP are aimed at understanding the origin of the process of protein synthesis. Aminoacyl (2',3') esters of 5'-AMP can serve as models of the 3'-terminus of aminoacyl tRNA. We report here on the relative rates of hydrolysis of Ac-D- and L-Phe AMP esters as a function of pH. At all pHs above 3, the rate constant of hydrolysis of the Ac-L-Phe ester is 1.7 to 2.1 times that of Ac-D-Phe ester. The D-isomer seems partially protected from hydrolysis by a stronger association with the adenine ring of the 5'-AMP.

  13. A click chemistry approach to glycomimetics: Michael addition of 2,3,4,6-tetra-O-acetyl-1-thio-beta-D-glucopyranose to 4-deoxy-1,2-O-isopropylidene-L-glycero-pent-4-enopyranos-3-ulose--a convenient route to novel 4-deoxy-(1-->5)-5-C-thiodisaccharides.

    Science.gov (United States)

    Witczak, Zbigniew J; Lorchak, David; Nguyen, Nguyen

    2007-09-03

    The base catalyzed conjugate Michael addition of the 1-thiosugar, 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranose, 1, to a new highly reactive enone 4-deoxy-1,2-O-isopropylidene-L-glycero-pent-4-enopyranos-3-ulose, 2, proceeds steroselectively with formation of adduct 3 in 94% yield. Convenient stereoselective reduction of the C-3 keto function of 3 with L-Selectride followed by in situ acetylation produces thiodisaccharide 4 in good 82% yield. Cleavage of the 1,2-O-isopropylidene protecting group with p-toluenesulfonic acid in methanol, followed by de-O-acetylation, produced an inseparable anomeric mixture of methyl 4-deoxy-5-C-(beta-D-glucopyranosyl)-thio-alpha/beta-L-ribo-pyranoside 5 in 72% overall yield. This approach constitutes a new general two-step click chemistry route to the previously unknown class of 4-deoxy-(1-->5)-5-C-thiodisaccharides as stable and biologically important glycomimetics.

  14. 2'-phosphodiesterase and 2',5'-oligoadenylate synthetase activities in the lowest metazoans, sponge [porifera

    DEFF Research Database (Denmark)

    Saby, Emilie; Poulsen, Jesper Buchhave; Justesen, Just

    2009-01-01

    Sponges [porifera], the most ancient metazoans, contain modules related to the vertebrate immune system, including the 2′,5′-oligoadenylate synthetase (OAS). The components of the antiviral 2′,5′-oligoadenylate (25A) system (OAS, 2′-Phosphodiesterase (2′-PDE) and RNAse L) of vertebrates have...... not all been identified in sponges. Here, we demonstrate for the first time that in addition to the OAS activity, sponges possess a 2′-PDE activity, which highlights the probable existence of a premature 25A system. Indeed, Suberites domuncula and Crella elegans exhibited this 25A degrading activity....... Upon this finding, two out of three elements forming the 25A system have been found in sponges, only a endoribonuclease, RNAse L or similar, has to be found. We suspect the existence of a complex immune system in sponges, besides the self/non-self recognition system and the use of phagocytosis...

  15. 2'-phosphodiesterase and 2',5'-oligoadenylate synthetase activities in the lowest metazoans, sponge [porifera].

    Science.gov (United States)

    Saby, Emilie; Poulsen, Jesper Buchhave; Justesen, Just; Kelve, Merike; Uriz, Maria Jesus

    2009-01-01

    Sponges [porifera], the most ancient metazoans, contain modules related to the vertebrate immune system, including the 2',5'-oligoadenylate synthetase (OAS). The components of the antiviral 2',5'-oligoadenylate (2-5A) system (OAS, 2'-Phosphodiesterase (2'-PDE) and RNAse L) of vertebrates have not all been identified in sponges. Here, we demonstrate for the first time that in addition to the OAS activity, sponges possess a 2'-PDE activity, which highlights the probable existence of a premature 2-5A system. Indeed, Suberites domuncula and Crella elegans exhibited this 2-5A degrading activity. Upon this finding, two out of three elements forming the 2-5A system have been found in sponges, only a endoribonuclease, RNAse L or similar, has to be found. We suspect the existence of a complex immune system in sponges, besides the self/non-self recognition system and the use of phagocytosis and secondary metabolites against pathogens.

  16. Electrochemical determination of 5-dodecylsalicylaldoxime and 2′-hydroxy-5′-nonylacetophenone oxime in commercial extractants

    Directory of Open Access Journals (Sweden)

    Elizalde María P.

    2017-01-01

    Full Text Available A differential pulse voltammetric method using a hanging mercury drop electrode (HMDE was optimized for the determination of 5-dodecylsalicylaldoxime in hydro-alcoholic solutions using factorial and central composite designs. The Taguchi design methodology was used to extend the formerly optimized method to the determination of 2′-hydroxy-5′-nonylacetophenone oxime. The method was successfully applied to quantify 5-dodecylsalicylaldoxime and 2′-hydroxy-5′-nonylacetophenone oxime in samples of commercial extractants over the concentration range 0.05–2.45 and 0.07–0.82 mg L-1, respectively, with detection limits of 0.034 and 0.019 mg L-1, respectively.

  17. Changes in 5-HT2A-mediated behavior and 5-HT2A- and 5-HT1A receptor binding and expression in conditional brain-derived neurotrophic factor knock-out mice

    DEFF Research Database (Denmark)

    Klein, A B; Santini, M A; Aznar, S

    2010-01-01

    Changes in brain-derived neurotrophic factor (BDNF) expression have been implicated in the etiology of psychiatric disorders. To investigate pathological mechanisms elicited by perturbed BDNF signaling, we examined mutant mice with central depletion of BDNF (BDNF(2L/2LCk-cre)). A severe impairment...... specific for the serotonin 2A receptor (5-HT(2A)R) in prefrontal cortex was described previously in these mice. This is of much interest, as 5-HT(2A)Rs have been linked to neuropsychiatric disorders and anxiety-related behavior. Here we further characterized the serotonin receptor alterations triggered...... was decreased in hippocampus of BDNF mutants, but unchanged in frontal cortex. Molecular analysis indicated corresponding changes in 5-HT(2A) and 5-HT(1A) mRNA expression but normal 5-HT(2C) content in these brain regions in BDNF(2L/2LCk-cre) mice. We investigated whether the reduction in frontal 5-HT(2A...

  18. Discovery of the young L dwarf wise J174102.78-464225.5

    International Nuclear Information System (INIS)

    Schneider, Adam C.; Cushing, Michael C.; Kirkpatrick, J. Davy; Mace, Gregory N.; Gelino, Christopher R.; Fajardo-Acosta, Sergio; Faherty, Jacqueline K.; Sheppard, Scott S.

    2014-01-01

    We report the discovery of the L dwarf WISE J174102.78–464225.5, which was discovered as part of a search for nearby L dwarfs using the Wide-field Infrared Survey Explorer (WISE). The distinct triangular peak of the H-band portion of its near-infrared spectrum and its red near-infrared colors (J – K S = 2.35 ± 0.08 mag) are indicative of a young age. Via comparison to spectral standards and other red L dwarfs, we estimate a near-infrared spectral type of L7 ± 2 (pec). From a comparison to spectral and low-mass evolutionary models, we determine self-consistent effective temperature, log g, age, and mass values of 1450 ± 100 K, 4.0 ± 0.25 (cm s –2 ), 10-100 Myr, and 4-21 M Jup , respectively. With an estimated distance of 10-30 pc, we explore the possibility that WISE J174102.78–464225.5 belongs to one of the young nearby moving groups via a kinematic analysis and we find potential membership in the β Pictoris or AB Doradus associations. A trigonometric parallax measurement and a precise radial velocity can help to secure its membership in either of these groups.

  19. Positron emission tomographic studies on aromatic L-amino acid decarboxylase activity in vivo for L-dopa and 5-hydroxy-L-tryptophan in the monkey brain

    Energy Technology Data Exchange (ETDEWEB)

    Hartvig, P; Tedroff, J; Lindner, K J; Bjurling, P; Chang, C W; Laangstroem, B [Uppsala Univ. (Sweden); Tsukada, H [Central Research Lab., Hamamatsu Photonics Shizuoka, Osaka (Japan); Watanabe, Y [Dept. of Neuroscience, Osaka Bioscience Inst., Osaka (Japan)

    1993-01-01

    The regional brain kinetics following 5-hydroxy-L-([beta]-11 C)tryptophan and L-([beta]-11 C)DOPA intravenous injection was measured in twelve Rhesus monkeys using positron emission tomography (PET). The radiolabelled compounds were also injected together with various doses of unlabelled 5-hydroxy-L-tryptophan or L-DOPA. The radioactivity accumulated in the striatal region and the rate of increased utilization with time was calculated using a graphical method with back of the brain as a reference region. The rate constants for decarboxylation were 0.0070 [+-] 0.0007 (S. D) and 0.0121 [+-] 0.0010 min[sup -1] for 5-hydroxy-L-([beta]-11 C)tryptophan and L-([beta]-11 C)DOPA, respectively. After concomitant injection with unlabelled 5-hydroxy-L-tryptophan, the rate constant of 5-hydroxy-L-([beta]-11 C)tryptophan decreased dose-dependently and a 50 percent reduction was seen with a dose of about 4 mg/kg of unlabelled compound. A decreased utilization rate of L-([beta]-11 C)DOPA was seen only after simultaneous injection of 30 mg/kg of either L-DOPA or 5-hydroxy-L-tryptophan. This capacity limitation was most likely interpreted as different affinity of the striatal aromatic amino acid decarboxylase for L-DOPA and 5-hydroxy-L-tryptophan, respectively.

  20. Do L2 Writing Courses Affect the Improvement of L1 Writing Skills via Skills Transfer from L2 to L1?

    Science.gov (United States)

    Gonca, Altmisdort

    2016-01-01

    This study investigates the relationship of second language (L2) writing skills proficiency with the first language (L1) writing skills, in light of the language transfer. The study aims to analyze the positive effects of L2 writing proficiency on L1 writing proficiency. Forty native Turkish-speaking university students participated in the study.…

  1. Econometric modelling of multiple self-reports of health states: The switch from EQ-5D-3L to EQ-5D-5L in evaluating drug therapies for rheumatoid arthritis.

    Science.gov (United States)

    Hernández-Alava, Mónica; Pudney, Stephen

    2017-09-01

    EQ-5D is used in cost-effectiveness studies underlying many important health policy decisions. It comprises a survey instrument describing health states across five domains, and a system of utility values for each state. The original 3-level version of EQ-5D is being replaced with a more sensitive 5-level version but the consequences of this change are uncertain. We develop a multi-equation ordinal response model incorporating a copula specification with normal mixture marginals to analyse joint responses to EQ-5D-3L and EQ-5D-5L in a survey of people with rheumatic disease, and use it to generate mappings between the alternative descriptive systems. We revisit a major cost-effectiveness study of drug therapies for rheumatoid arthritis, mapping the original EQ-5D-3L measure onto a 5L valuation basis. Working within a comprehensive, flexible econometric framework, we find that use of simpler restricted specifications can make very large changes to cost-effectiveness estimates with serious implications for decision-making. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  2. Magnetic behavior of MnPS3 phases intercalated by [Zn2L]2+ (LH2: macrocyclic ligand obtained by condensation of 2-hydroxy-5-methyl-1,3-benzenedicarbaldehyde and 1,2-diaminobenzene)

    International Nuclear Information System (INIS)

    Spodine, E.; Valencia-Galvez, P.; Fuentealba, P.; Manzur, J.; Ruiz, D.; Venegas-Yazigi, D.; Paredes-Garcia, V.; Cardoso-Gil, R.; Schnelle, W.; Kniep, R.

    2011-01-01

    The intercalation of the cationic binuclear macrocyclic complex [Zn 2 L] 2+ (LH 2 : macrocyclic ligand obtained by the template condensation of 2-hydroxy-5-methyl-1,3-benzenedicarbaldehyde and 1,2-diaminobenzene) was achieved by a cationic exchange process, using K 0.4 Mn 0.8 PS 3 as a precursor. Three intercalated materials were obtained and characterized: (Zn 2 L) 0.05 K 0.3 Mn 0.8 PS 3 (1), (Zn 2 L) 0.1 K 0.2 Mn 0.8 PS 3 (2) and (Zn 2 L) 0.05 K 0.3 Mn 0.8 PS 3 (3), the latter phase being obtained by an assisted microwave radiation process. The magnetic data permit to estimate the Weiss temperature θ of ∼-130 K for (1); ∼-155 K for (2) and ∼-130 K for (3). The spin canting present in the potassium precursor remains unperturbed in composite (3), and spontaneous magnetization is observed under 50 K in both materials. However composites (1) and (2) do not present this spontaneous magnetization at low temperatures. The electronic properties of the intercalates do not appear to be significantly altered. The reflectance spectra of the intercalated phases (1), (2) and (3) show a gap value between 1.90 and 1.80 eV, lower than the value observed for the K 0.4 Mn 0.8 PS 3 precursor of 2.8 eV. -- Graphical Abstract: Microwave assisted synthesis was used to obtain an intercalated MnPS 3 phase with a binuclear Zn(II) macrocyclic complex. A comparative magnetic study of the composites obtained by assisted microwave and traditional synthetic methods is reported. Display Omitted Highlights: → A rapid and efficient preparation of intercalated MnPS 3 composites by assisted microwave synthesis is described. → The exchange of potassium ions of the precursor by the macrocyclic Zn(II) complex is partial. → The composite obtained by assisted microwave synthesis retains the spontaneous magnetization, observed in the low temperature range of the magnetic susceptibility of the potassium precursor. → The materials obtained by the conventional method loose the spontaneous

  3. Dr Jekyll and Mr Hyde: a strange case of 5-ethynyl-2 '-deoxyuridine and 5-ethynyl-2 '- deoxycytidine

    Czech Academy of Sciences Publication Activity Database

    Ligasová, A.; Liboska, Radek; Friedecký, D.; Mičová, K.; Adam, T.; Oždian, T.; Rosenberg, Ivan; Koberna, K.

    2016-01-01

    Roč. 6, č. 1 (2016), č. článku 150172. ISSN 2046-2441 R&D Projects: GA MZd NV15-31604A Institutional support: RVO:61388963 Keywords : cytidine deaminase * dCMP deaminase * 5-ethynyl-2 '-deoxyuridine * 5-ethynyl-2 '-deoxycytidine * DNA replication Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.481, year: 2016 http://rsob.royalsocietypublishing.org/content/6/1/150172

  4. Aspergillus niger PA2: a novel strain for extracellular biotransformation of L-tyrosine into L-DOPA.

    Science.gov (United States)

    Agarwal, Pragati; Pareek, Nidhi; Dubey, Swati; Singh, Jyoti; Singh, R P

    2016-05-01

    L-DOPA (3,4-dihydroxyphenyl-L-alanine), an amino acid derivative is the most widely used drug of choice for the treatment of Parkinson's disease and other neurologic injuries. The present study deals with the elevated biochemical transformation of L-tyrosine to L-DOPA by Aspergillus niger PA2, a potent tyrosinase producer, isolated from decomposed food wastes. This appears to be the first report on A. niger as a notable extracellular tyrosinase producer. The extracellular tyrosinase activity produced remarkably higher levels of L-DOPA, i.e. 2.44 mg mL(-1) when the media was supplemented with 5 mg mL(-1) L-tyrosine. The optimum pH for tyrosinase production was 6.0, with the maximal L-DOPA production at the same pH. The product thus produced was analyzed by thin-layer chromatography, UV spectroscopy, high-performance liquid chromatography and Fourier transform infrared spectroscopy, that had denoted this to be L-DOPA. Kinetic parameters viz. Y p/s, Q s and Q p had further indicated the notable levels of production. Thus, Aspergillus niger PA2 could be a promising resource and may be further exploited for large-scale production of L-DOPA.

  5. Induction bending of API 5L X80 pipes; Curvamento a quente de tubos API 5L X80

    Energy Technology Data Exchange (ETDEWEB)

    Batista, Gilmar Z. [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil)]. E-mail: g.zacca@petrobras.com.br; Naschpitz, Leonardo [Primus Processamento de Tubos S.A. - PROTUBO, Campo Grande, RJ (Brazil)]. E-mail: naschpitz@protubo.com.br

    2005-07-01

    The present work is a part of an extensive program to make possible the application of API 5L X80 in pipeline construction in Brazil. At this stage, the effect of the induction bending process on the microstructure and mechanical properties of the pipe is presented. For this study an API 5L X80 pipe, made by the UOE process, is used. The pipe was manufactured from a plate produced by thermo-mechanically controlled rolling without accelerated cooling. The pipe bending was carried out applying a local induction heating following by water quenching. The bend section, outer and inner curvature regions, of the pipe bend were evaluated and compared with the original pipe. The longitudinal weld and transition zones were not evaluated at this stage. Dimensional analyses, microstructural evaluation, Charpy-V impact tests, and tensile tests were performed. A significant microstructural change was verified in the bend area. The yield strength of the pipe bend was found to be lower than original pipe and standard requirements. Other tensile properties and impact properties are compatible with API 5L X80 requirements. (author)

  6. X-ray crystal and molecular structure of (eta/sup 5/C/sub 5/H/sub 5/)UCl/sub 3/. 2PPh/sub 3/O thf: trichloro (eta/sup 5/cyclopentadienyl)bis(triphenylphosphine oxide) uranium(IV). thf

    Energy Technology Data Exchange (ETDEWEB)

    Bombieri, G; De Paoli, G [Consiglio Nazionale delle Ricerche, Padua (Italy). Lab. di Chimica e Tecnologia dei Radioelementi; Del Pra, A; Bagnall, K W

    1978-01-01

    Following reports of the preparation of (eta/sup 5/C/sub 5/H/sub 5/)UCl/sub 3/.xL (x = 1, L = dimethoxyethane and x = 2, L = tetrahydrofuran (thf), a series of analogous oxygen-donor complexes were isolated. This paper describes the crystal and molecular structure of one of them, ((eta/sup 5/C/sub 5/H/sub 5/)UCl/sub 3/.2PPh/sub 3/O)thf.

  7. Thirteen week toxicity study of dietary l-tryptophan in rats with a recovery period of 5 weeks.

    Science.gov (United States)

    Shibui, Yusuke; Matsumoto, Hideki; Masuzawa, Yoko; Ohishi, Takumi; Fukuwatari, Tsutomu; Shibata, Katsumi; Sakai, Ryosei

    2018-04-01

    Although l-tryptophan is nutritionally important and widely used in medical applications, toxicity data for its oral administration are limited. The purpose of this study was to evaluate the potential toxicity of an experimental diet containing added l-tryptophan at doses of 0 (basal diet), 1.25%, 2.5% and 5.0% when administered to Sprague-Dawley rats for 13 weeks. There were no toxicological changes in clinical signs, ophthalmology, urinalysis, hematology, necropsy, organ weight and histopathology between control rats and those fed additional l-tryptophan. Body weight gain and food consumption significantly decreased throughout the administration period in males in the 2.5% group and in both sexes in the 5.0% group. At the end of the dosing period, decreases in water intake in males in the 5.0% group and in serum glucose in females in the 5.0% group were observed. The changes described above were considered toxicologically significant; however, they were not observed after a 5 week recovery period, suggesting reversibility. Consequently, the no-observed-adverse-effect level of l-tryptophan in the present study was 1.25% for males and 2.5% for females (mean intake of l-tryptophan: 779 mg kg -1 body weight day -1 [males] and 1765 mg kg -1 body weight day -1 [females]). As the basal diet used in this study contained 0.27% of proteinaceous l-tryptophan, the no-observed-adverse-effect level of overall l-tryptophan was 1.52% for males and 2.77% for females (mean intake of overall l-tryptophan: 948 mg kg -1 body weight day -1 (males) and 1956 mg kg -1 body weight day -1 (females)). We conclude that l-tryptophan has a low toxicity profile in terms of human use. Copyright © 2017 John Wiley & Sons, Ltd.

  8. Quality of life profile of general Vietnamese population using EQ-5D-5L.

    Science.gov (United States)

    Nguyen, Long Hoang; Tran, Bach Xuan; Hoang Le, Quynh Ngoc; Tran, Tung Thanh; Latkin, Carl A

    2017-10-11

    Health-related quality of life (HRQOL) is a vital benchmark to assess the effects of health interventions and policies. Measuring HRQOL of the general population is essential to establish a reference for health outcomes evaluations. However, evidence on HRQOL of general populations in low and middle income countries is very limited. This study aimed to measure HRQOL of the Vietnamese population by using the EuroQol-5 dimensions-5 levels (EQ-5D-5L) instrument and determine its associated factors. A cross-sectional study was performed in Hanoi with 1571 residences in Hanoi, the capital city of Vietnam. EQ-5D-5L and EQ- visual analogue scale (EQ-VAS) were used to assess HRQOL. Potential covariates included socio-demographic characteristics, having acute symptoms in the last four weeks, chronic diseases in the last three months, having multiple health issues, and health service utilisation in the last twelve months. A generalized linear model was employed to identify the association between HRQOL and covariates. Overall, the mean EQ-5D utility index was 0.91 (SD = 0.15), and the mean EQ-VAS score was 87.4 (SD = 14.3). The highest proportion of respondents reporting any problems was in Usual activities (24.3%), followed by Anxiety/Depression (15.2%) and Pain/Discomfort (10.0%), while the lowest percentage was in Self-care (2.5%). Lower HRQOL composite scores were related to unemployment, lower income, higher education, living in urban areas, having chronic diseases, having multiple health issues and using health service. For any health problem self-reported by respondents, the health utility reduced by 0.02 (respiratory diseases) to 0.15 (musculoskeletal diseases). Health utility of the general population and reductions for self-reported health problems in this study are useful for future population health evaluations and comparisons. It also informs the development of interventions to reduce health problems of the general population.

  9. Xenon spectator and diagram L3-M4,5M4,5 Auger intensities near the L3 threshold

    International Nuclear Information System (INIS)

    Armen, G.B.; Levin, J.C.; Southworth, S.H.; LeBrun, T.; Arp, U.; MacDonald, M.A.

    1997-01-01

    Calculations based on the theory of radiationless resonant Raman scattering are employed in the interpretation of new XeL 3 -M 4,5 M 4,5 Auger spectra recorded using synchrotron radiation tuned to energies across the L 3 edge. Fits of theoretical line shapes to the spectra are employed in separating intensities due to nd spectator (resonant) and diagram Auger processes. Near-threshold Auger intensity, previously attributed to diagram decay, is found to be due to the large-n spectator lines that result from postcollision-interaction endash induced open-quotes recaptureclose quotes of threshold photoelectrons to nd orbitals. copyright 1997 The American Physical Society

  10. Post CHF heat transfer and quenching

    International Nuclear Information System (INIS)

    Nelson, R.A.; Condie, K.G.

    1980-01-01

    This paper describes quantitatively new mechanisms in the post-CHF regime which provide understanding and predictive capability for several current two-phase forced convective heat transfer problems. These mechanisms are important in predicting rod temperature turnaround and quenching during the reflood phase of either a hypothetical loss-of-coolant accident (LOCA) or the FLECHT and Semiscale experiments. The mechanisms are also important to the blowdown phase of a LOCA or the recent Loss-of-Fluid Test (LOFT) experiments L2-2 and L2-3, which were 200% cold leg break transients. These LOFT experiments experienced total core quenching in the early part of the blowdown phase at high (1000 psia) pressures. The mechanisms are also important to certain pressurized water reactor (PWR) operational transients where the reactor may operate in the post-CHF regime for short periods of time. Accurate prediction of the post-CHF heat transfer including core quench during these transients is of prime importance to limit maximum cladding temperatures and prevent cladding deformation

  11. Determinação de 2,5-hexanodiona em urina empregando cromatografia líquida de alta eficiência, após derivatização com 2,4-dinitrofenil-hidrazina

    Directory of Open Access Journals (Sweden)

    Marina Venzon Antunes

    2011-01-01

    Full Text Available A method for quantifying urinary 2,5-hexanedione was optimized and validated. Urine samples were hydrolyzed and derivatized with 2,4-dinitrophenylhydrazine. The analyte was separated in a high performance liquid chromatography system with a diode array detector, using a C18 column (150 x 4.6 mm, p.d. 5 µm and a mobile phase composed of phosphate buffer pH 2.3:acetonitrile (40:60, v/v, at a flow rate of 1 mL/min. The chromatograms were monitored at 334 nm. Retention time was 7.3 minutes. Main validation parameters were: coefficient of determination: 0.9994, accuracy: 96 to 107%; intra-assay precision (RSD: 3.08 to 6.72%; inter-assay precision (RSD: 2.54 to 8.17% and limit of quantitation of 0.19 µg/mL.

  12. The Perihelion Emission of Comet C/2010 L5 ( WISE )

    Energy Technology Data Exchange (ETDEWEB)

    Kramer, E. A.; Bauer, J. M.; Stevenson, R.; Mainzer, A. K.; Masiero, J.; Sonnett, S. [Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, Pasadena, CA 91109 (United States); Fernandez, Y. R. [University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816 (United States); Grav, T. [Planetary Science Institute, 1700 East Fort Lowell, Suite 106, Tucson, AZ 85719-2395 (United States); Nugent, C. [Infrared Processing and Analysis Center, California Institute of Technology, Pasadena, CA 91125 (United States)

    2017-03-20

    The only Halley-type comet discovered by the Wide-Field Infrared Survey Explorer ( WISE ), C/2010 L5 ( WISE ), was imaged three times by WISE , and it showed a significant dust tail during the second and third visits (2010 June and July, respectively). We present here an analysis of the data collected by WISE , putting estimates on the comet’s size, dust production rate, gas production (CO+CO{sub 2}) rate, and active fraction. We also present a detailed description of a novel tail-fitting technique that allows the commonly used syndyne–synchrone models to be used analytically, thereby giving more robust results. We find that C/2010 L5's dust tail was likely formed by strong emission, likely in the form of an outburst, occurring when the comet was within a few days of perihelion. Analyses of the June and July data independently agree on this result. The two separate epochs of dust tail analysis independently suggest a strong emission event close to perihelion. The average size of the dust particles in the dust tail increased between the epochs, suggesting that the dust was primarily released in a short period of time, and the smaller dust particles were quickly swept away by solar radiation pressure, leaving the larger particles behind. The difference in CO{sub 2} and dust production rates measured in 2010 June and July is not consistent with “normal” steady-state gas production from a comet at these heliocentric distances, suggesting that much of the detected CO{sub 2} and dust was produced in an episodic event. Together, these conclusions suggest that C/2010 L5 experienced a significant outburst event when the comet was close to perihelion.

  13. The Perihelion Emission of Comet C/2010 L5 ( WISE )

    International Nuclear Information System (INIS)

    Kramer, E. A.; Bauer, J. M.; Stevenson, R.; Mainzer, A. K.; Masiero, J.; Sonnett, S.; Fernandez, Y. R.; Grav, T.; Nugent, C.

    2017-01-01

    The only Halley-type comet discovered by the Wide-Field Infrared Survey Explorer ( WISE ), C/2010 L5 ( WISE ), was imaged three times by WISE , and it showed a significant dust tail during the second and third visits (2010 June and July, respectively). We present here an analysis of the data collected by WISE , putting estimates on the comet’s size, dust production rate, gas production (CO+CO 2 ) rate, and active fraction. We also present a detailed description of a novel tail-fitting technique that allows the commonly used syndyne–synchrone models to be used analytically, thereby giving more robust results. We find that C/2010 L5's dust tail was likely formed by strong emission, likely in the form of an outburst, occurring when the comet was within a few days of perihelion. Analyses of the June and July data independently agree on this result. The two separate epochs of dust tail analysis independently suggest a strong emission event close to perihelion. The average size of the dust particles in the dust tail increased between the epochs, suggesting that the dust was primarily released in a short period of time, and the smaller dust particles were quickly swept away by solar radiation pressure, leaving the larger particles behind. The difference in CO 2 and dust production rates measured in 2010 June and July is not consistent with “normal” steady-state gas production from a comet at these heliocentric distances, suggesting that much of the detected CO 2 and dust was produced in an episodic event. Together, these conclusions suggest that C/2010 L5 experienced a significant outburst event when the comet was close to perihelion.

  14. The structure of carbon nanotubes formed of graphene layers L4-8, L5-7, L3-12, L4-6-12

    Science.gov (United States)

    Shapovalova, K. E.; Belenkov, E. A.

    2017-11-01

    We geometrically calculate the optimized structure of nanotubes based on the graphene layers, using the method of molecular mechanics MM+. It was found that only the nanotubes, based on the graphene layers L4-8, L5-7, L3-12, L4-6-12, have a cylindrical form. Calculations of the sublimation energy, carried out using the semi-empirical quantum-mechanic method PM3, show that energy increases with the increase of nanotube diameters.

  15. Radiosynthesis of dimethyl-2-[{sup 18}F]-(fluoromethyl)-6-methyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate for L-type calcium channel imaging

    Energy Technology Data Exchange (ETDEWEB)

    Sadeghpour, H. [Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School (AMIRS), Karaj (Iran); Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran Univ. of Medical Sciences, Tehran (Iran); Jalilian, A.R.; Akhlaghi, M.; Mirzaei, M. [Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School (AMIRS), Karaj (Iran); Shafiee, A. [Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran Univ. of Medical Sciences, Tehran (Iran); Miri, R. [Medicinal and Natural Products Chemistry Research Center, Shiraz Univ. of Medical Sciences, Shiraz (Iran)

    2008-07-01

    Dimethyl 2-(fluoromethyl)-6-methyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate 4a, a fluorinated nifedipine analog, has been shown to elicit significant calcium channel blocker activity using a guinea pig ileal longitudinal smooth muscle model. In order to perform biological studies for detection of L-type calcium channel distribution, we decided to prepare the [{sup 18}F]-labeled compound. The latter compound was prepared in no-carrier-added (n.c.a.) form from dimethyl 2-(bromomethyl)-6-methyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate 2 in one step at 80 C in Kryptofix[222]/K[{sup 18}F]F and acetonitrile as a solvent in 15 min. Column chromatography afforded the radiochemically pure compound in 20 min. Radiochemical purity of the {sup 18}F-nifedipine was determined by RTLC and HPLC (> 98%) and specific activity of 21-48 GBq/{mu}mol (EOB). (orig.)

  16. L’italiano L2 : auto-apprendimento e narratività. Materiali multimediali di italiano L2 per bambini e ragazzi.

    Directory of Open Access Journals (Sweden)

    Graziella Favaro

    2009-12-01

    Full Text Available Normal 0 14 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabella normale"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman";} Normal 0 14 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabella normale"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman";} Insegnare l'italiano come seconda lingua ai bambini e ragazzi di recente immigrazione è un percorso  delicato e complesso che  fa ormai parte  dei compiti professionali ";ordinari"; dei docenti che operano nelle classi sempre più multiculturali e plurilingui. La modalità di insegnamento/apprendimento della L2 più diffusa ed efficace è quella integrata: l'alunno non italofono è inserito nella classe comune e, parallelamente, in determinati momenti, frequenta il laboratorio  di italiano L2. Per  sostenere  questo modello integrato e accompagnare il  viaggio dentro l'italiano dei bambini e dei ragazzi neoarrivati, servono materiali didattici flessibili e ricchi di proposte, utilizzabili in maniera autonoma e in piccolo gruppo; proponibili in maniera tradizionale o anche attraverso la LIM; adatti a sviluppare competenze diverse, proprie della lingua per comunicare, dell'italiano scritto e propedeutiche all'apprendimento del curricolo comune. I due corsi di italiano L2  che vengono presentati nell'ultima parte dell'articolo rispondono a queste esigenze, in quanto a sono rivolti l'uno ai bambini e l'altro ai ragazzi stranieri neo arrivati; b si propongono  il passaggio dal livello prebasico ai livelli A1, A2 e B1 del QCERL; c si

  17. The effect of dynamic solar shading on energy, daylighting and thermal comfort in a nearly zero-energy loft room in Rome and Copenhagen

    DEFF Research Database (Denmark)

    Skarning, Gunnlaug Cecilie Jensen; Hviid, Christian Anker; Svendsen, Svend

    2017-01-01

    alternatives in buildings with very low space-heating demand, this study mapped and compared energy, daylighting and thermal comfort for various combinations of window size and glazing properties, with and without dynamic shading. The study considered a loft room with sloped roof windows and moderate venting...... as defined by the Adaptive Thermal Comfort (ATC) model....... options in nearly zero-energy homes in Rome and Copenhagen. The more flexible solution space with dynamic shading made it possible to either reduce the time with operative temperatures exceeding the comfort limit by 40–50 h or increase daylighting by 750–1000 h more than could be achieved without shading...

  18. Trojan asteroids - Populations, dynamical structure and origin of the L4 and L5 swarms

    International Nuclear Information System (INIS)

    Shoemaker, E.M.; Shoemaker, C.S.; Wolfe, R.F.

    1989-01-01

    The origin of Trojan asteroids, their populations, and dynamical structures are examined. Data available of Trojan asteroids reveal that the total population of Trojans of greater than 15-km diam is roughly half that estimated for the main-belt asteroids. Two-thirds of the known Trojans are in the L4 swarm. Bright Trojans are as numerous in the L5 swarm as in L4 swarm, but faint L5 Trojans are only half as numerous. Similarities of characteristic orbital parameters among certain Trojans indicate the presence of five and possibly as many as eight collisional groups in the L4 swarm. It is suggested that the magnitude distribution of L4 Trojans is probably a result of strong collisional evolution. It is suggested that the present Trojans are chiefly fragments of Jupiter planetesimals that were captured during an episode of heavy flux near Jupiter during the dispersal of the planetesimal swarm. 40 refs

  19. Lutheri loft / Irene Roos

    Index Scriptorium Estoniae

    Roos, Irene

    2008-01-01

    Tallinnas Lutheri kvartalis läbi kahe korruse paiknevast näidiskorterist. Sisustajad Irene Roos ja Ester Penjam. Paekiviseina kasutati sisustuselemendina, Philipsi valgustid jagasid ruumi eri tsoonideks. 9 värv. ill

  20. Discussion on the method to increase the successful rate of L5/S1 intervertebral disc puncture

    International Nuclear Information System (INIS)

    Zhong Xianyi; Li Liangjun; Yu Chengxin

    2007-01-01

    Objective: To study the effective methods of L 5 /S 1 intervertebral disc puncture without drilling to solve the barriers from iliaca. Methods: (1) puncturing with belly-buttock sticking out: to enlarge waist sacro-iliaca angle to move the puncture point up; (2) puncturing through intervertebral edge: puncturing through L 5 to 1/3 intervertebral disc to make the puncture point move Up; (3) puncturing through L 5 /S 1 intervertebral disc with the self-made puncture location instrument. Results with the methods, 280 cases with L 5 /S 1 intervertebral disc protrusion have been successfully punctured, with successful rate 100%. Conclusion: These methods are ideal and easy to use to treat L 5 /S 1 intervertebral disc protrusion puncture, and worth popularizing. (authors)

  1. Synthesis and characterization of technetium(III) complexes containing 2,2'-bipyridine and 1,10-phenanthroline. X-ray crystal structures of cis (Cl),trans(P)-[TcCl2(P(CH3)2C6H5)2(bpy)]B(C6H5)4, cis (Cl),trans(P)-[TcCl2(P(CH3)2C6H5)2(phen)]B(C6H5)4, and cis (Cl),trans(P)-[TcCl2(P(CH3CH2)(C6H5)2)2(bpy)]SO3CF3

    International Nuclear Information System (INIS)

    Wilcox, B.E.; Ho, D.M.; Deutsch, E.

    1989-01-01

    Technetium(III) complexes of the general formula cis(Cl),trans(P)-[TcCl 2 (P) 2 L] + , where (P) is dimethylphenylphosphine (PMe 2 Ph) or ethyldiphenylphosphine (PEtPh 2 ) and L is 2,2'-bipyridine (bpy), 4,4'-dimethyl-2,2'-bipyridine (Me 2 bpy), or 1,10-phenanthroline (phen), have been synthesized and characterized. They are prepared by L substitution onto, with concomitant displacement of one chloride and one phosphine ligand from, the mer-TcCl 3 (P) 3 starting material in refluxing ethanol. Analysis of these complexes by fast atom bombardment mass spectrometry (in the positive ion mode) provides fingerprint mass spectra that exhibit peaks assigned to the molecular ion M + as well as peaks assigned to M + minus one or more monodentate ligands. Results of single-crystal x-ray structure determinations of cis(Cl),trans(P)-[TcCl 2 (PMe 2 Ph) 2 (bpy)]BPh 4 (A), cis(Cl),trans(P)-[TcCl 2- (PMe 2 Ph) 2 (phen)]BPh 4 (B), and cis(Cl),trans(P)-[TcCl 2 (PEtPh 2 ) 2 (bpy)]SO 3 CF 3 (C), with formula weights of 921.62, 945.64, and 903.65, respectively are reported. 26 refs., 5 figs., 5 tabs

  2. Atividade de três drogas antivirais sobre os herpesvírus bovino tipos 1, 2 e 5 em cultivo celular Activity of three antiviral drugs against bovine herpesviruses 1, 2 and 5 in cell culture

    Directory of Open Access Journals (Sweden)

    Renata Dezengrini

    2010-10-01

    Full Text Available A atividade de três fármacos antivirais (Aciclovir [ACV], Ganciclovir [GCV] e Foscarnet [PFA] foi testada in vitro frente aos herpesvírus bovino tipos 1 (BoHV-1, 2 (BoHV-2 e 5 (BoHV-5. Para isso, utilizou-se o teste de reducao de placas virais em cultivo celular, testando-se diferentes concentracoes dos farmacos frente a 100 doses infectantes para 50% dos cultivos celulares (DICC50 dos respectivos virus. Pelo teste de MTT (3-(4,5-Dimethylthiazol- 2-yl-2,5-diphenyltetrazolium bromide, verificou-se que concentracoes inferiores a 200ƒÊg/mL dos tres antivirais resultaram em indices de viabilidade de celulas MDBK e Hep2 superiores a 80%. Com base na concentracao citotoxica para 50% das celulas (CC50 e na concentracao dos farmacos efetiva para inibir em 50% o numero de placas virais (EC50, calculou-se o indice de seletividade (IS dos antivirais para os tres herpesvirus. Assim, o ACV demonstrou ser moderadamente ativo frente ao BoHV-1 (EC50: 112,9ƒÊg/mL e IS: 4,5, ao BoHV-2 (EC50: 114,2 ƒÊg/mL e IS: 4,5 e BoHV-5 (EC50: 96,9ƒÊg/mL e IS: 5,3. O GCV apresentou atividade moderada frente ao BoHV-2 (EC50: 33,5ƒÊg/mL e IS: 16,6 e, em menor grau, contra o BoHV-5 (EC50: 123,2ƒÊg/mL e IS: 4,5, sendo ineficaz frente ao BoHV-1 (EC50: 335,8ƒÊg/mL e IS: 1,7. O PFA apresentou atividade antiviral mais pronunciada, sendo o unico farmaco que, na concentracao de 100ƒÊg/mL, inibiu completamente a producao de placas pelos tres virus testados. O PFA foi o mais efetivo in vitro frente ao BoHV-1 (EC50: 29,5ƒÊg/mL e IS: 42,2, ao BoHV-2 (EC50: 45,2ƒÊg/mL e IS: 27,6 e ao BoHV-5 (EC50: 7,8ƒÊg/mL e IS: 160,6. Portanto, os resultados obtidos indicam que o PFA pode se constituir em um candidato para terapia experimental de infeccoes pelos herpesvirus de bovinos in vivo.The activity of three anti-herpetic drugs (Acyclovir [ACV], Gancyclovir [GCV] and Foscarnet [PFA] was tested against bovine herpesvirus 1 (BoHV-1, 2 (BoHV-2 and 5 (BoHV-5 in vitro using the

  3. 3,5-Diiodo-L-thyronine (3,5-t2) exerts thyromimetic effects on hypothalamus-pituitary-thyroid axis, body composition, and energy metabolism in male diet-induced obese mice.

    Science.gov (United States)

    Jonas, Wenke; Lietzow, Julika; Wohlgemuth, Franziska; Hoefig, Carolin S; Wiedmer, Petra; Schweizer, Ulrich; Köhrle, Josef; Schürmann, Annette

    2015-01-01

    Effective and safe antiobesity drugs are still needed in face of the obesity pandemic worldwide. Recent interventions in rodents revealed 3,5-diiodo-L-thyronine (3,5-T2) as a metabolically active iodothyronine affecting energy and lipid metabolism without thyromimetic side effects typically associated with T3 administration. Accordingly, 3,5-T2 has been proposed as a potential hypolipidemic agent for treatment of obesity and hepatic steatosis. In contrast to other observations, our experiments revealed dose-dependent thyromimetic effects of 3,5-T2 akin to those of T3 in diet-induced obese male C57BL/6J mice. 3,5-T2 treatment exerted a negative feedback regulation on the hypothalamus-pituitary-thyroid axis, similar to T3. This is demonstrated by decreased expression of genes responsive to thyroid hormones (TH) in pituitary resulting in a suppressed thyroid function with lower T4 and T3 concentrations in serum and liver of 3,5-T2-treated mice. Analyses of hepatic TH target genes involved in lipid metabolism revealed T3-like changes in gene expression and increased type I-deiodinase activity after application of 3,5-T2 (2.5 μg/g body weight). Reduced hepatic triglyceride and serum cholesterol concentrations reflected enhanced lipid metabolism. Desired increased metabolic rate and reduction of different fat depots were, however, compromised by increased food intake preventing significant body weight loss. Moreover, enlarged heart weights indicate potential cardiac side effects of 3,5-T2 beyond hepatic thyromimetic actions. Altogether, the observed thyromimetic effects of 3,5-T2 in several mouse TH target tissues raise concern about indiscriminate administration of 3,5-T2 as powerful natural hormone for the treatment of hyperlipidemia and pandemic obesity.

  4. Elicitation effects of synthetic 1,2,4,5-tetraoxane and 2,5-diphenyltiophene in shoot cultures of two Nepeta species

    Directory of Open Access Journals (Sweden)

    Dmitrović Slavica

    2016-01-01

    Full Text Available The present study was aimed to investigate the elicitation possibility of the main secondary metabolites production in Nepeta cataria L. and N. pannonica L. plants, by exposing them to synthetic compounds from the group of tetraoxanes and tiophenes. The effect of DO63 (1,2,4,5-tetraoxane and DOVF15 (2,5-diphenyl-tiophene on the production of cis,trans-nepetalactone (NL and rosmarinic acid (RA in two Nepeta species, was investigated in shoots grown on culture medium with addition of synthetic compounds in concentrations ranging from 0.1 to 2 mg L-1. The content of targeted metabolites in tested in vitro shoots depended on the type and the concentration of applied synthetic compounds. Application of DO63, primarily in concentration of 0.1 mg L-1 to 1 mg L-1, affected only NL production in both Nepeta species resulting in increased NL content in treated shoots, while production of RA was not influenced. Addition of DOVF15 caused decrease of RA content in N. pannonica shoots and increase in N. cataria shoots, whereas NL production was not affected. The presented results highlight the possibility of DO63 and DOVF15 application for the elicitation of the main secondary metabolites production in species from the genus Nepeta. [Projekat Ministarstva nauke Republike Srbije, br. 172008 i br. 173024

  5. The Effects of L2 Experience on L3 Perception

    Science.gov (United States)

    Onishi, Hiromi

    2016-01-01

    This study examines the influence of experience with a second language (L2) on the perception of phonological contrasts in a third language (L3). This study contributes to L3 phonology by examining the influence of L2 phonological perception abilities on the perception of an L3 at the beginner level. Participants were native speakers of Korean…

  6. COMPARISON BETWEEN FENTANYL 2 μG/mL VERSUS DEXMEDETOMIDINE 1.5 μG/mL AS ADJUVANTS WITH ISOBARIC BUPIVACAINE 0.0625% IN EPIDURAL LABOUR ANALGESIA

    Directory of Open Access Journals (Sweden)

    Karuna Harsoor

    2016-11-01

    Full Text Available BACKGROUND Low-dose bupivacaine with opioids like fentanyl have been used traditionally as an adjunct for epidural labour analgesia, but has side effects like pruritus, urinary retention, nausea, vomiting, etc. Dexmedetomidine, an α-2 adrenergic agonist with a high placental retention, decreased sympathetic outflow and norepinephrine release thereby causing sedation, anxiolysis, analgesia, sympatholysis and maternal haemodynamic stability. Hence, this study was carried out to compare dexmedetomidine and fentanyl as adjuvants in epidural labour analgesia. MATERIALS AND METHODS A prospective randomised, double-blind study was conducted in 60 term parturients in active labour of ASA I and II physical status. They were randomised into 2 groups with 30 parturients in each and received 15 mL of 0.0625% Inj. Bupivacaine plus either 1.5 μg/mL Inj. Dexmedetomidine (BD group or 2 μg/mL Inj. Fentanyl (BF group. VAS, sedation scores, modified four grade Bromage scale, haemodynamics, peripheral oxygen saturation were recorded at baseline and regular intervals. When VAS was ≥4, subsequent doses of 5 mL of the respective group drug was administered. Parturients were ambulated when Bromage scale was 0 with no postural hypotension. Duration of analgesia, labour outcome, neonatal Apgar scores and side effects were noted. RESULTS Demography and haemodynamic stability were similar and comparable. Duration of analgesia was significantly longer in BD group (131.83±45.760 than BF group (85.33±22.512 (p<0.0001. More no. of top-ups was needed in BF group (1.80±1.518 than BD group (0.17±0.461 (p<0.0001. Significantly reduced VAS scores was observed in BD group than BF group. Mean sedation scores (p<0.05 and maximum Bromage scores (p=0.004 were significantly higher in BD group than BF group. Ambulation was less in BD group (3 parturients than BF group (26 parturients. Side effects were significantly more in BF group than BD group (p=0.007. The labour outcomes

  7. A Novel Association between Two Trypanosome-Specific Factors and the Conserved L5-5S rRNA Complex

    Science.gov (United States)

    Ciganda, Martin; Prohaska, Kimberly; Hellman, Kristina; Williams, Noreen

    2012-01-01

    P34 and P37 are two previously identified RNA binding proteins in the flagellate protozoan Trypanosoma brucei. RNA interference studies have determined that the proteins are involved in and essential for ribosome biogenesis. The proteins interact with the 5S rRNA with nearly identical binding characteristics. We have shown that this interaction is achieved mainly through the LoopA region of the RNA, but P34 and P37 also protect the L5 binding site located on LoopC. We now provide evidence to show that these factors form a novel pre-ribosomal particle through interactions with both 5S rRNA and the L5 ribosomal protein. Further in silico and in vitro analysis of T. brucei L5 indicates a lower affinity for 5S rRNA than expected, based on other eukaryotic L5 proteins. We hypothesize that P34 and P37 complement L5 and bridge the interaction with 5S rRNA, stabilizing it and aiding in the early steps of ribosome biogenesis. PMID:22859981

  8. Contribución de fibras mielínicas provenientes de los nervios espinales lumbares L4, L5 y L6 al nervio ciático de rata adulta y sus ramas principales Contribution of myelunated fibers from spinal L4, L5 and L6 nerves to the sciatic nerve and its main branches in the adult rat

    Directory of Open Access Journals (Sweden)

    Hernán Hurtado

    2000-04-01

    Full Text Available El nervio ciático de la rata está formado por los nervios espinales (ne lumbares L4, L5 y L6. Sin embargo, aún no se ha definido el aporte en fibras mielínicas de estos nervios espinales a lo largo del tronco nervioso. En este estudio se transectaron selectivamente los NE L4, L5 y L4-L5. Luego de una semana se disecaron los nervios ciático, tibial, sural y peroneal. Estas muestras se fijaron y procesaron para microscopía óptica y a partir de cortes coloreados con azul de toluidina se contaron las fibras mielínicas degeneradas y normales. L4 contribuyó con fibras mielínicas principalmente al nervio peroneal y L5 a los nervios ciático, tibial y sural. En general, el aporte de L6 fue menor y variable a lo largo del tronco nervioso comparado con las otras dos ramas espinales. Nuestros resultados brindan información valiosa para posteriores estudios que busquen correlacionar la contribución de los nervios espinales que componen el ciático y sus ramas principales con la función de la extremidad inferior. The rat sciatic nerve is composed by the L4, L5 and L6 lumbar spinal nerves. However, the contribution in myelinated fibers originating from these nerves along this nervous trunk has not yet been defined. In the present study, the L4, L5 and L4-L5 spinal nerves were selectively transected. After one week the sciatic, tibial, sural and peroneal nerves were dissected. These samples were fixed and processed for optical microscopy, and both degenerated and normal myelinated fibers were counted in toluidine blue-stained semi-thin sections. L4 contributed with myelinated fibers mainly to the peroneal nerve, and L5 to the sciatic, tibial and sural nerves. In general, the contribution of L6 was smaller and variable along the nervous trunk in comparison to the other two spinal branches. Our results give key information for further studies looking to correlate the contribution of spinal nerves making part of the sciatic nerve and its main

  9. Detailed analysis of RNA-protein interactions within the bacterial ribosomal protein L5/5S rRNA complex.

    OpenAIRE

    Perederina, Anna; Nevskaya, Natalia; Nikonov, Oleg; Nikulin, Alexei; Dumas, Philippe; Yao, Min; Tanaka, Isao; Garber, Maria; Gongadze, George; Nikonov, Stanislav

    2002-01-01

    The crystal structure of ribosomal protein L5 from Thermus thermophilus complexed with a 34-nt fragment comprising helix III and loop C of Escherichia coli 5S rRNA has been determined at 2.5 A resolution. The protein specifically interacts with the bulged nucleotides at the top of loop C of 5S rRNA. The rRNA and protein contact surfaces are strongly stabilized by intramolecular interactions. Charged and polar atoms forming the network of conserved intermolecular hydrogen bonds are located in ...

  10. Synthesis, characterisation and antibacterial activity of [(p-cym)RuX(L)](+/2+) (X = Cl, H2O; L = bpmo, bpms) complexes.

    Science.gov (United States)

    Tripathy, Suman Kumar; Taviti, Ashoka Chary; Dehury, Niranjan; Sahoo, Anupam; Pal, Satyanaryan; Beuria, Tushar Kant; Patra, Srikanta

    2015-03-21

    Mononuclear half-sandwiched complexes [(p-cym)RuCl(bpmo)](ClO4) {[1](ClO4)} and [(p-cym)RuCl(bpms)](PF6) {[2](PF6)} have been prepared by reacting heteroscorpionate ligands bpmo = 2-methoxyphenyl-bis(3,5-dimethylpyrazol-1-yl)methane and bpms = 2-methylthiophenyl-bis(3,5-dimethylpyrazol-1-yl)methane, respectively, with a dimeric precursor complex [(p-cym)RuCl(μ-Cl)]2 (p-cym = 1-isopropyl-4-methylbenzene) in methanol. The corresponding aqua derivatives [(p-cym)Ru(H2O)(bpmo)](ClO4)2 {[3](ClO4)2} and [(p-cym)Ru(H2O)(bpms)](PF6)2 {[4](PF6)2} are obtained from {[1](ClO4)} and {[2](PF6)}, respectively, via Cl(-)/H2O exchange process in the presence of appropriate equivalents of AgClO4/AgNO3 + KPF6 in a methanol-water mixture. The molecular structures of the complexes {[1]Cl, [3](ClO4)2 and [4](PF6)(NO3)} are authenticated by their single crystal X-ray structures. The complexes show the expected piano-stool geometry with p-cym in the η(6) binding mode. The aqua complexes [3](ClO4)2 and [4](PF6)2 show significantly good antibacterial activity towards E. coli (gram negative) and B. subtilis (gram positive) strains, while chloro derivatives ({[1](ClO4)} and {[2](PF6)} are found to be virtually inactive. The order of antibacterial activity of the complexes according to their MIC values is [1](ClO4) (both 1000 μg mL(-1)) < [2](PF6) (580 μg mL(-1) and 750 μg mL(-1)) < [3](ClO4)2 (both 100 μg mL(-1)) < [4](PF6)2 (30 μg mL(-1) and 60 μg mL(-1)) for E. coli and B. subtilis strains, respectively. Further, the aqua complexes [3](ClO4)2 and [4](PF6)2 show clear zones of inhibition against kanamycin, ampicillin and chloramphenicol resistant E. coli strains. The detailed mechanistic aspects of the aforesaid active aqua complexes [3](ClO4)2 and [4](PF6)2 have been explored, and it reveals that both the complexes inhibit the number of nucleoids per cell in vivo and bind to DNA in vitro. The results indeed demonstrate that both [3](ClO4)2 and [4](PF6)2 facilitate the inhibition of

  11. CT volumetry of lumbar vertebral bodies in patients with hypoplasia L5 and bilateral spondylolysis and in normal controls

    International Nuclear Information System (INIS)

    Wilms, Guido E.; Demaerel, Philippe; Keyzer, Frederik de; Willems, Endry

    2012-01-01

    To examine the feasibility and results of calculating the volume of lumbar vertebral bodies in normal patients and patients with suspected hypoplasia of L5. Lumbar multi-detector CT was performed in 38 patients with bilateral spondylolysis and hypoplasia of L5 and in 38 normal patients. Lumbar vertebral body volume of L3, L4 and L5 was measured by CT volumetry with a semi-automated program, created with MeVisLab. In the control group, the average vertebral body volume (in cubic centimeters) of L3 was 35.93 (±7.33), 36.34 (±7.13) for L4 and 34.63 (±6.88) for L5. In patients with suspected hypoplasia L5 the average body volume (in cubic centimeters) of L3 was 36.85 (±7.37), 36.90 (±6.99) for L4 and 33.14 (±6.57) for L5. The difference in mean vertebral body volume for L3, L4 and L5 between both groups was statistically not significant. However, there was a statistically significant difference of the ratio L5/L4 (P < 0.001) between both groups: the mean ratio L5/L4 in the control group was 95.3 ± 3.9%, the ratio for the hypoplastic L5 group was 89.9 ± 6.3%. There was no significant difference in the vertebral body volume for L3, L4 and L5 between both groups due to inter-patient variability. However, the relation between the body volume of L5 and L4 is significantly different between both groups. The volume of the vertebral body of L5 proved to be on average 10.2% smaller than the volume of L4 in the group with hypoplasia L5 versus 4.7% in the control group. (orig.)

  12. CT volumetry of lumbar vertebral bodies in patients with hypoplasia L5 and bilateral spondylolysis and in normal controls

    Energy Technology Data Exchange (ETDEWEB)

    Wilms, Guido E.; Demaerel, Philippe; Keyzer, Frederik de [UZ Leuven, Campus Gasthuisberg, Department of Radiology, Leuven (Belgium); Willems, Endry [ZOL, Department of Radiology, Genk (Belgium)

    2012-08-15

    To examine the feasibility and results of calculating the volume of lumbar vertebral bodies in normal patients and patients with suspected hypoplasia of L5. Lumbar multi-detector CT was performed in 38 patients with bilateral spondylolysis and hypoplasia of L5 and in 38 normal patients. Lumbar vertebral body volume of L3, L4 and L5 was measured by CT volumetry with a semi-automated program, created with MeVisLab. In the control group, the average vertebral body volume (in cubic centimeters) of L3 was 35.93 ({+-}7.33), 36.34 ({+-}7.13) for L4 and 34.63 ({+-}6.88) for L5. In patients with suspected hypoplasia L5 the average body volume (in cubic centimeters) of L3 was 36.85 ({+-}7.37), 36.90 ({+-}6.99) for L4 and 33.14 ({+-}6.57) for L5. The difference in mean vertebral body volume for L3, L4 and L5 between both groups was statistically not significant. However, there was a statistically significant difference of the ratio L5/L4 (P < 0.001) between both groups: the mean ratio L5/L4 in the control group was 95.3 {+-} 3.9%, the ratio for the hypoplastic L5 group was 89.9 {+-} 6.3%. There was no significant difference in the vertebral body volume for L3, L4 and L5 between both groups due to inter-patient variability. However, the relation between the body volume of L5 and L4 is significantly different between both groups. The volume of the vertebral body of L5 proved to be on average 10.2% smaller than the volume of L4 in the group with hypoplasia L5 versus 4.7% in the control group. (orig.)

  13. Dysplastic L5-S1 Spondyloptosis in a 3-Year-Old Child: A Case Report and Review of the Literature

    Directory of Open Access Journals (Sweden)

    Vikas Tandon

    2017-01-01

    Full Text Available A three-year-old girl presented with primary complaint of severe low back pain with radiation to both lower limbs below the knees since 2 months following history of fall and marked restriction of her daily routine activities. After clinicoradiological evaluation she was diagnosed of having dysplastic L5-S1 spondyloptosis. A staged procedure was planned after thorough discussion with her parents. During initial stage she underwent posterior decompression along L5-S1 segment including exposure of bilateral L5 and S1 nerve roots followed by instrumented reduction (L3-S2 5.5 mm pedicle screws utilizing a rotational-translational technique. No interbody fusion was done at L5-S1 level and inner nuts of bilateral L3, L4, and S2 screws were intentionally kept loose. Subsequently after about symptom-free three-year follow up, she presented with recurrence of symptoms and underwent revision surgery as per initial plan discussed with her parents. Removals of posterior implants were done followed by stabilization with larger diameter pedicle screws (6.5 mm at L5 and S1 level. During the same stage through anterior transperitoneal approach L5-S1 interbody fusion was done. At one-year follow-up after second-stage definitive surgery, patient remains symptom-free and fully active without any radiological evidence of reduction loss or implant failure.

  14. The conversion of phenylalanine to tyrosine in man. Direct measurement by continuous intravenous tracer infusions of L-[ring-2H5]phenylalanine and L-[1-13C] tyrosine in the postabsorptive state

    International Nuclear Information System (INIS)

    Clarke, J.T.; Bier, D.M.

    1982-01-01

    Steady state phenylalanine and tyrosine turnover and the rate of conversion of phenylalanine of tyrosine in vivo were determined in 6 healthy postabsorptive adult volunteers. Continuous infusions of tracer amounts of L-[ring- 2 H5]phenylalanine were determined intravenously for 13-14 hr. After 9-10 hr, a priming dose followed by a continuous infusion of L-[1- 13 C]tyrosine was added and maintained, along with the [ 2 H5]phenylalanine infusion, for 4 hr. Venous plasma samples were obtained before the initiation of each infusion and every 30 min during the course of the combined [ 2 H5]phenylalanine and [ 13 C]tyrosine infusion for determination of isotopic enrichments of [ 2 H5]phenylalanine, [ 13 C]tyrosine, and [ 2 H4]tyrosine by gas chromatograph-mass spectrometric analysis of the N-trifluoroacetyl-, methyl ester derivatives of the amino acids. Calculated from the observed enrichments, free phenylalanine and tyrosine turnover rates were 36.1 +/- 5.1 mumole . kg-1 . h-1 and 39.8 +/- 3.5 mumole . kg-1 . h-1, respectively. Phenylalanine was converted to tyrosine at the rate of 5.83 +/- 0.59 mumole . kg-1 . h-1, accounting for approximately 16% of either the phenylalanine or the tyrosine flux. The results indicate that the normal basal steady state phenylalanine hydroxylase activity in vivo in man is lower than that obtained from phenylalanine loading studies. This supports the existence of some type of substance activation of the enzyme as reflected in the previously reported exponential relationship between phenylalanine concentration and phenylalanine hydroxylase activity in vitro. The use of continuous simultaneous infusions of tracer amounts of stable isotope-labeled phenylalanine and tyrosine provides a direct means for studying physiological regulation of phenylalanine hydroxylase activity in vivo

  15. Estudo do efeito da tensão sobre a permeação por Hidrogênio em Aços API 5L X60 e API 5L X65

    Directory of Open Access Journals (Sweden)

    Bruno Araújo Araújo

    2017-12-01

    Full Text Available Under operating conditions, the oil and gas transmission pipelines can be subjected to high pressures and, at the same time, suffer the effect of acidic substances in the transported hydrocarbons such as H2S, whose action alone or combined with stresses acting in the structure can cause degradation processes of the mechanical properties of the material. Given the importance of the phenomenon, this work has as main objective the realization of hydrogen electrochemical permeation tests in API 5L X60 and API 5L X65 steels under mechanical loading conditions. A device for performing electrochemical permeation tests of hydrogen in the specimens subjected to stress was used for the tests. Thus, it was possible to observe experimentally the influence of stress on the diffusion properties during the experiment. By applying stress on the order of 20% of the yield strength, it was possible to verify the increase of diffusivity of API 5L X60 steel. Furthermore, there was a strong influence of the stress level of permeability, because a large increase of permeability of API 5L X65 steel. The results indicate that the lattice deformation due to imposed mechanical stress has a great influence on the diffusion properties of materials

  16. Knock-in human GDF5 proregion L373R mutation as a mouse model for proximal symphalangism.

    Science.gov (United States)

    Zhang, Xinxin; Xing, Xuesha; Liu, Xing; Hu, Yu; Qu, Shengqiang; Wang, Heyi; Luo, Yang

    2017-12-26

    Proximal symphalangism (SYM1) is an autosomal dominant disorder, mainly characterized by bony fusions of the proximal phalanges of the hands and feet. GDF5 and NOG were identified to be responsible for SYM1. We have previously reported on a p.Leu373Arg mutation in the GDF5 proregion present in a Chinese family with SYM1. Here, we investigated the effects of the GDF-L373R mutation. The variant caused proteolysis efficiency of GDF5 increased in ATDC5 cells. The variant also caused upregulation of SMAD1/5/8 phosphorylation and increased expression of target genes SMURF1 , along with COL2A1 and SOX9 which are factors associated with chondrosis. Furthermore, we developed a human-relevant SYM1 mouse model by making a Gdf5 L367R (the orthologous position for L373R in humans) knock-in mouse. Gdf5 L367R/+ and Gdf5 L367R/L367R mice displayed stiffness and adhesions across the proximal phalanx joint which were in complete accord with SYM1. It was also confirmed the joint formation and development was abnormal in Gdf5 L367R/+ and Gdf5 L367R/L367R mice, including the failure to develop the primary ossification center and be hypertrophic chondrocytes during embryonic development. This knock-in mouse model offers a tool for assessing the pathogenesis of SYM1 and the function of the GDF5 proregion.

  17. Biotransformation of 5-hydroxy-methylfurfural into 2,5-furan-dicarboxylic acid by bacterial isolate using thermal acid algal hydrolysate.

    Science.gov (United States)

    Yang, Chu-Fang; Huang, Ci-Ruei

    2016-08-01

    Thermal acid hydrolysis is often used to deal with lignocellulosic biomasses, but 5-hydroxy-methylfurfural (5-HMF) formed during hydrolysis deeply influences downstream fermentation. 2,5-Furan-dicarboxylic acid (FDCA), which is in the list of future important biomass platform molecules can be obtained using 5-HMF biotransformation. Based on the connection between 5-HMF removal in acid hydrolysate and FDCA production, the optimum thermal acid hydrolysis condition for macroalgae Chaetomorpha linum was established. Potential microbes capable of transforming 5-HMF into FDCA were isolated and characterized under various parameters and inoculated into algal hydrolysate to perform 5-HMF biotransformation. The optimum hydrolysis condition was to apply 0.5M HCl to treat 3% algal biomass under 121°C for 15min. Isolated Burkholderia cepacia H-2 could transform 2000mg/L 5-HMF at the initial pH of 7 at 28°C and 1276mg/L FDCA was received. Strain B. cepacia H-2 was suitable for treating the algal hydrolysate without dilution, receiving 989.5mg/L FDCA. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Low doses of flagellin-L2 multimer vaccines protect against challenge with diverse papillomavirus genotypes.

    Science.gov (United States)

    Kalnin, Kirill; Tibbitts, Timothy; Yan, Yanhua; Stegalkina, Svetlana; Shen, Lihua; Costa, Victor; Sabharwal, Robert; Anderson, Stephen F; Day, Patricia M; Christensen, Neil; Schiller, John T; Jagu, Subhashini; Roden, Richard B S; Almond, Jeffrey; Kleanthous, Harold

    2014-06-12

    Genetically modified bacterial flagellin (Fla), a Toll-like receptor-5 (TLR5) ligand, was evaluated as a fusion partner for human papillomavirus (HPV) L2-based immunogens in two animal challenge models; either cutaneous inoculation of rabbits with HPV 'quasivirions' containing cottontail rabbit papillomavirus (CRPV) genomes that induce warts, or intra-vaginal inoculation of mice with HPV 'pseudovirions' encapsidating a luciferase reporter plasmid and measurement of bioluminescence to determine infectivity. An Escherichia coli production system was developed for flagellin-L2 (Fla-L2) fusions containing either monomeric HPV-16 L2 a.a. 11(×11-200) or oligomeric L2 comprising a fusion of the a.a. 11-88 peptides of five (Fla∼5×11-88) or eight (Fla∼8×11-88) genital HPV types. Immunogenicity and bioactivity of Fla-L2 constructs were assessed using an in vitro neutralization and cell-based TLR-5 binding assay, respectively. Efficacy was evaluated following active immunization of rabbits or mice administered 3 intramuscular doses of Fla-L2 recombinants without exogenous adjuvant, followed by challenge. In addition, passive immunization studies of naïve rabbits with serial dilutions of pooled immune sera were used to determine End-Point Protection Titers (EPPT) for each formulation against a broader spectrum of HPV quasivirions. Efficacy was assessed for up to 10 weeks on the basis of wart volume induced following challenge and results compared to licensed L1-VLP vaccines (Gardasil and Cervarix). Following active immunization at doses as low as 1 μg, Fla-L2 fusions afforded complete protection against infection (mice) and disease (rabbits) following either homologous or heterologous HPV challenge. Passive immunization with anti-L2 immune sera discriminated between the different vaccine candidates under evaluation, demonstrated the protective role of antibody and suggested the superiority of this oligomeric L2-TLR5 agonist fusion approach compared to L1-based

  19. Permeability and toxicity characteristics of L-cysteine and 2-methyl-thiazolidine-4-carboxylic acid in Caco-2 cells.

    Science.gov (United States)

    Kartal-Hodzic, Alma; Marvola, Tuuli; Schmitt, Mechthild; Harju, Kirsi; Peltoniemi, Marikki; Sivén, Mia

    2013-01-01

    Acetaldehyde is a known mutagenic substance and has been classified as a group-one carcinogen by the WHO. It is possible to bind acetaldehyde locally in the gastrointestinal (GI) tract with the semi-essential amino acid l-cysteine, which reacts covalently with acetaldehyde and forms compound 2-methyl-thiozolidine-4-carboxylic acid (MTCA). The Caco-2 cell line was used to determine the permeation of l-cysteine and MTCA, as well as the possible cell toxicity of both substances. Neither of the substances permeated through the Caco-2 cells at the concentrations used in this study, and only the highest concentration of MTCA affected the viability of the cells in the MTT (3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide) test. These results showed that when l-cysteine is administered in formulations releasing it locally in the lower parts of GI tract, it is not absorbed but can react with acetaldehyde, and that neither l-cysteine nor MTCA is harmful to the cells when present locally in the upper parts of GI tract. This study also shows that MTCA is sensitive at a lower pH of 5.5. Since stable MTCA is desired in different parts of the GI tract, this observation raises concern over the influence of lower pH on l-cysteine-containing product ability to bind and eliminate carcinogenic acetaldehyde.

  20. Impact of genetic polymorphisms of SLC2A2, SLC2A5, and KHK on metabolic phenotypes in hypertensive individuals.

    Directory of Open Access Journals (Sweden)

    MyPhuong T Le

    Full Text Available In the past few decades, consumption of added sugars has increased dramatically. Studies have linked high sugar intake with increased risk for a number of diseases. Importantly, fructose, a component of sugar, has been linked with the development of features of metabolic syndrome. This study determined if single nucleotide polymorphisms in genes involved in fructose transport (solute carrier family 2 facilitated glucose transporter, member 2 (SLC2A2 and solute carrier family 2 facilitated glucose/fructose transporter, member 5 (SLC2A5 and metabolism (ketohexokinase (KHK affect inter-individual variability in metabolic phenotypes, such as increased serum uric acid levels.The influence of SLC2A2, SLC2A5, and KHK SNPs on metabolic phenotypes was tested in 237 European Americans and 167 African Americans from the Pharmacogenomic Evaluation and Antihypertensive Responses (PEAR study. Using baseline untreated fasting data, associations were considered significant if p≤0.005. These SNPs were then evaluated for potential replication (p≤0.05 using data from the Genetic Epidemiology of Responses to Antihypertensives (GERA studies.SLC2A5 rs5438 was associated with an increase in serum uric acid in European American males. However, we were unable to replicate the association in GERA. The minor allele of SLC2A2 rs8192675 showed an association with lower high-density lipoproteins in European Americans (A/A: 51.0 mg/dL, A/G: 47.0 mg/dL, G/G: 41.5 mg/dL, p = 0.0034 in PEAR. The association between rs8192675 and lower high-density lipoproteins was replicated in the combined European American GERA study samples (A/A: 47.6 mg/dL, A/G: 48.6 mg/dL, G/G: 41.9 mg/dL, p = 0.0315.The association between SLC2A2 rs8192675 and high-density lipoproteins suggests the polymorphism may play a role in influencing high-density lipoproteins and thus metabolic risk of cardiovascular disease.

  1. Synthesis, growth, structural, optical and thermal properties of a new organic nonlinear optical crystal: 2-amino 5-chloropyridinium-L-tartarate

    Science.gov (United States)

    Jayanalina, T.; Rajarajan, G.; Boopathi, K.; Sreevani, K.

    2015-09-01

    A new organic nonlinear optical crystal 2-amino-5-chloropyridinium-L-tartarate [2A5CPLTA] has been synthesized and the crystals were grown by slow evaporation solution technique at room temperature using methanol as solvent. The crystal structure of the title compound has been determined by the single crystal X-ray diffraction study and it belongs to the monoclinic system with noncentrosymmetric space group P21. The presence of functional groups was ascertained by Fourier transform infrared analysis. The transmittance and lower cut off of the grown crystal was ascertained by the UV-vis-NIR spectroscopy. Thermal studies reveled that 2A5CPLTA crystal is thermally stable up to 144 °C. The dielectric measurements of the grown crystal were carried out with different frequencies and temperatures. Vickers micro hardness measurement was carried out to study the mechanical behavior of the grown crystal. The second harmonic generation of the title crystal was confirmed by the Kurtz-Perry powder test employing the Nd: YAG laser as the source.

  2. Measurement of the ZZ production cross section and search for anomalous couplings in 2l2l' final states in pp collisions at $\\sqrt{s}$=7 TeV

    CERN Document Server

    Chatrchyan, Serguei; Sirunyan, Albert M; Tumasyan, Armen; Adam, Wolfgang; Aguilo, Ernest; Bergauer, Thomas; Dragicevic, Marko; Erö, Janos; Fabjan, Christian; Friedl, Markus; Fruehwirth, Rudolf; Ghete, Vasile Mihai; Hammer, Josef; Hörmann, Natascha; Hrubec, Josef; Jeitler, Manfred; Kiesenhofer, Wolfgang; Knünz, Valentin; Krammer, Manfred; Krätschmer, Ilse; Liko, Dietrich; Mikulec, Ivan; Pernicka, Manfred; Rahbaran, Babak; Rohringer, Christine; Rohringer, Herbert; Schöfbeck, Robert; Strauss, Josef; Taurok, Anton; Waltenberger, Wolfgang; Walzel, Gerhard; Widl, Edmund; Wulz, Claudia-Elisabeth; Mossolov, Vladimir; Shumeiko, Nikolai; Suarez Gonzalez, Juan; Bansal, Monika; Bansal, Sunil; Cornelis, Tom; De Wolf, Eddi A; Janssen, Xavier; Luyckx, Sten; Mucibello, Luca; Ochesanu, Silvia; Roland, Benoit; Rougny, Romain; Selvaggi, Michele; Staykova, Zlatka; Van Haevermaet, Hans; Van Mechelen, Pierre; Van Remortel, Nick; Van Spilbeeck, Alex; Blekman, Freya; Blyweert, Stijn; D'Hondt, Jorgen; Gonzalez Suarez, Rebeca; Kalogeropoulos, Alexis; Maes, Michael; Olbrechts, Annik; Van Doninck, Walter; Van Mulders, Petra; Van Onsem, Gerrit Patrick; Villella, Ilaria; Clerbaux, Barbara; De Lentdecker, Gilles; Dero, Vincent; Gay, Arnaud; Hreus, Tomas; Léonard, Alexandre; Marage, Pierre Edouard; Mohammadi, Abdollah; Reis, Thomas; Thomas, Laurent; Vander Marcken, Gil; Vander Velde, Catherine; Vanlaer, Pascal; Wang, Jian; Adler, Volker; Beernaert, Kelly; Cimmino, Anna; Costantini, Silvia; Garcia, Guillaume; Grunewald, Martin; Klein, Benjamin; Lellouch, Jérémie; Marinov, Andrey; Mccartin, Joseph; Ocampo Rios, Alberto Andres; Ryckbosch, Dirk; Strobbe, Nadja; Thyssen, Filip; Tytgat, Michael; Walsh, Sinead; Yazgan, Efe; Zaganidis, Nicolas; Basegmez, Suzan; Bruno, Giacomo; Castello, Roberto; Ceard, Ludivine; Delaere, Christophe; Du Pree, Tristan; Favart, Denis; Forthomme, Laurent; Giammanco, Andrea; Hollar, Jonathan; Lemaitre, Vincent; Liao, Junhui; Militaru, Otilia; Nuttens, Claude; Pagano, Davide; Pin, Arnaud; Piotrzkowski, Krzysztof; Schul, Nicolas; Vizan Garcia, Jesus Manuel; Beliy, Nikita; Caebergs, Thierry; Daubie, Evelyne; Hammad, Gregory Habib; Alves, Gilvan; Correa Martins Junior, Marcos; Martins, Thiago; Pol, Maria Elena; Henrique Gomes E Souza, Moacyr; Aldá Júnior, Walter Luiz; Carvalho, Wagner; Custódio, Analu; Melo Da Costa, Eliza; De Jesus Damiao, Dilson; De Oliveira Martins, Carley; Fonseca De Souza, Sandro; Matos Figueiredo, Diego; Mundim, Luiz; Nogima, Helio; Prado Da Silva, Wanda Lucia; Santoro, Alberto; Soares Jorge, Luana; Sznajder, Andre; Vilela Pereira, Antonio; Souza Dos Anjos, Tiago; Bernardes, Cesar Augusto; De Almeida Dias, Flavia; Tomei, Thiago; De Moraes Gregores, Eduardo; Lagana, Caio; Da Cunha Marinho, Franciole; Mercadante, Pedro G; Novaes, Sergio F; Padula, Sandra; Genchev, Vladimir; Iaydjiev, Plamen; Piperov, Stefan; Rodozov, Mircho; Stoykova, Stefka; Sultanov, Georgi; Tcholakov, Vanio; Trayanov, Rumen; Vutova, Mariana; Dimitrov, Anton; Hadjiiska, Roumyana; Kozhuharov, Venelin; Litov, Leander; Pavlov, Borislav; Petkov, Peicho; Bian, Jian-Guo; Chen, Guo-Ming; Chen, He-Sheng; Jiang, Chun-Hua; Liang, Dong; Liang, Song; Meng, Xiangwei; Tao, Junquan; Wang, Jian; Wang, Xianyou; Wang, Zheng; Xiao, Hong; Xu, Ming; Zang, Jingjing; Zhang, Zhen; Asawatangtrakuldee, Chayanit; Ban, Yong; Guo, Yifei; Li, Wenbo; Liu, Shuai; Mao, Yajun; Qian, Si-Jin; Teng, Haiyun; Wang, Dayong; Zhang, Linlin; Zou, Wei; Avila, Carlos; Gomez, Juan Pablo; Gomez Moreno, Bernardo; Osorio Oliveros, Andres Felipe; Sanabria, Juan Carlos; Godinovic, Nikola; Lelas, Damir; Plestina, Roko; Polic, Dunja; Puljak, Ivica; Antunovic, Zeljko; Kovac, Marko; Brigljevic, Vuko; Duric, Senka; Kadija, Kreso; Luetic, Jelena; Morovic, Srecko; Attikis, Alexandros; Galanti, Mario; Mavromanolakis, Georgios; Mousa, Jehad; Nicolaou, Charalambos; Ptochos, Fotios; Razis, Panos A; Finger, Miroslav; Finger Jr, Michael; Assran, Yasser; Elgammal, Sherif; Ellithi Kamel, Ali; Mahmoud, Mohammed; Radi, Amr; Kadastik, Mario; Müntel, Mait; Raidal, Martti; Rebane, Liis; Tiko, Andres; Eerola, Paula; Fedi, Giacomo; Voutilainen, Mikko; Härkönen, Jaakko; Heikkinen, Mika Aatos; Karimäki, Veikko; Kinnunen, Ritva; Kortelainen, Matti J; Lampén, Tapio; Lassila-Perini, Kati; Lehti, Sami; Lindén, Tomas; Luukka, Panja-Riina; Mäenpää, Teppo; Peltola, Timo; Tuominen, Eija; Tuominiemi, Jorma; Tuovinen, Esa; Ungaro, Donatella; Wendland, Lauri; Banzuzi, Kukka; Karjalainen, Ahti; Korpela, Arja; Tuuva, Tuure; Besancon, Marc; Choudhury, Somnath; Dejardin, Marc; Denegri, Daniel; Fabbro, Bernard; Faure, Jean-Louis; Ferri, Federico; Ganjour, Serguei; Givernaud, Alain; Gras, Philippe; Hamel de Monchenault, Gautier; Jarry, Patrick; Locci, Elizabeth; Malcles, Julie; Millischer, Laurent; Nayak, Aruna; Rander, John; Rosowsky, André; Shreyber, Irina; Titov, Maksym; Baffioni, Stephanie; Beaudette, Florian; Benhabib, Lamia; Bianchini, Lorenzo; Bluj, Michal; Broutin, Clementine; Busson, Philippe; Charlot, Claude; Daci, Nadir; Dahms, Torsten; Dalchenko, Mykhailo; Dobrzynski, Ludwik; Florent, Alice; Granier de Cassagnac, Raphael; Haguenauer, Maurice; Miné, Philippe; Mironov, Camelia; Naranjo, Ivo Nicolas; Nguyen, Matthew; Ochando, Christophe; Paganini, Pascal; Sabes, David; Salerno, Roberto; Sirois, Yves; Veelken, Christian; Zabi, Alexandre; Agram, Jean-Laurent; Andrea, Jeremy; Bloch, Daniel; Bodin, David; Brom, Jean-Marie; Cardaci, Marco; Chabert, Eric Christian; Collard, Caroline; Conte, Eric; Drouhin, Frédéric; Ferro, Cristina; Fontaine, Jean-Charles; Gelé, Denis; Goerlach, Ulrich; Juillot, Pierre; Le Bihan, Anne-Catherine; Van Hove, Pierre; Fassi, Farida; Mercier, Damien; Beauceron, Stephanie; Beaupere, Nicolas; Bondu, Olivier; Boudoul, Gaelle; Chasserat, Julien; Chierici, Roberto; Contardo, Didier; Depasse, Pierre; El Mamouni, Houmani; Fay, Jean; Gascon, Susan; Gouzevitch, Maxime; Ille, Bernard; Kurca, Tibor; Lethuillier, Morgan; Mirabito, Laurent; Perries, Stephane; Sgandurra, Louis; Sordini, Viola; Tschudi, Yohann; Verdier, Patrice; Viret, Sébastien; Tsamalaidze, Zviad; Anagnostou, Georgios; Autermann, Christian; Beranek, Sarah; Calpas, Betty; Edelhoff, Matthias; Feld, Lutz; Heracleous, Natalie; Hindrichs, Otto; Jussen, Ruediger; Klein, Katja; Merz, Jennifer; Ostapchuk, Andrey; Perieanu, Adrian; Raupach, Frank; Sammet, Jan; Schael, Stefan; Sprenger, Daniel; Weber, Hendrik; Wittmer, Bruno; Zhukov, Valery; Ata, Metin; Caudron, Julien; Dietz-Laursonn, Erik; Duchardt, Deborah; Erdmann, Martin; Fischer, Robert; Güth, Andreas; Hebbeker, Thomas; Heidemann, Carsten; Hoepfner, Kerstin; Klingebiel, Dennis; Kreuzer, Peter; Merschmeyer, Markus; Meyer, Arnd; Olschewski, Mark; Papacz, Paul; Pieta, Holger; Reithler, Hans; Schmitz, Stefan Antonius; Sonnenschein, Lars; Steggemann, Jan; Teyssier, Daniel; Thüer, Sebastian; Weber, Martin; Bontenackels, Michael; Cherepanov, Vladimir; Erdogan, Yusuf; Flügge, Günter; Geenen, Heiko; Geisler, Matthias; Haj Ahmad, Wael; Hoehle, Felix; Kargoll, Bastian; Kress, Thomas; Kuessel, Yvonne; Lingemann, Joschka; Nowack, Andreas; Perchalla, Lars; Pooth, Oliver; Sauerland, Philip; Stahl, Achim; Aldaya Martin, Maria; Behr, Joerg; Behrenhoff, Wolf; Behrens, Ulf; Bergholz, Matthias; Bethani, Agni; Borras, Kerstin; Burgmeier, Armin; Cakir, Altan; Calligaris, Luigi; Campbell, Alan; Castro, Elena; Costanza, Francesco; Dammann, Dirk; Diez Pardos, Carmen; Eckerlin, Guenter; Eckstein, Doris; Flucke, Gero; Geiser, Achim; Glushkov, Ivan; Gunnellini, Paolo; Habib, Shiraz; Hauk, Johannes; Hellwig, Gregor; Jung, Hannes; Kasemann, Matthias; Katsas, Panagiotis; Kleinwort, Claus; Kluge, Hannelies; Knutsson, Albert; Krämer, Mira; Krücker, Dirk; Kuznetsova, Ekaterina; Lange, Wolfgang; Lohmann, Wolfgang; Lutz, Benjamin; Mankel, Rainer; Marfin, Ihar; Marienfeld, Markus; Melzer-Pellmann, Isabell-Alissandra; Meyer, Andreas Bernhard; Mnich, Joachim; Mussgiller, Andreas; Naumann-Emme, Sebastian; Novgorodova, Olga; Olzem, Jan; Perrey, Hanno; Petrukhin, Alexey; Pitzl, Daniel; Raspereza, Alexei; Ribeiro Cipriano, Pedro M; Riedl, Caroline; Ron, Elias; Rosin, Michele; Salfeld-Nebgen, Jakob; Schmidt, Ringo; Schoerner-Sadenius, Thomas; Sen, Niladri; Spiridonov, Alexander; Stein, Matthias; Walsh, Roberval; Wissing, Christoph; Blobel, Volker; Draeger, Jula; Enderle, Holger; Erfle, Joachim; Gebbert, Ulla; Görner, Martin; Hermanns, Thomas; Höing, Rebekka Sophie; Kaschube, Kolja; Kaussen, Gordon; Kirschenmann, Henning; Klanner, Robert; Lange, Jörn; Mura, Benedikt; Nowak, Friederike; Peiffer, Thomas; Pietsch, Niklas; Rathjens, Denis; Sander, Christian; Schettler, Hannes; Schleper, Peter; Schlieckau, Eike; Schmidt, Alexander; Schröder, Matthias; Schum, Torben; Seidel, Markus; Sibille, Jennifer; Sola, Valentina; Stadie, Hartmut; Steinbrück, Georg; Thomsen, Jan; Vanelderen, Lukas; Barth, Christian; Berger, Joram; Böser, Christian; Chwalek, Thorsten; De Boer, Wim; Descroix, Alexis; Dierlamm, Alexander; Feindt, Michael; Guthoff, Moritz; Hackstein, Christoph; Hartmann, Frank; Hauth, Thomas; Heinrich, Michael; Held, Hauke; Hoffmann, Karl-Heinz; Husemann, Ulrich; Katkov, Igor; Komaragiri, Jyothsna Rani; Lobelle Pardo, Patricia; Martschei, Daniel; Mueller, Steffen; Müller, Thomas; Niegel, Martin; Nürnberg, Andreas; Oberst, Oliver; Oehler, Andreas; Ott, Jochen; Quast, Gunter; Rabbertz, Klaus; Ratnikov, Fedor; Ratnikova, Natalia; Röcker, Steffen; Schilling, Frank-Peter; Schott, Gregory; Simonis, Hans-Jürgen; Stober, Fred-Markus Helmut; Troendle, Daniel; Ulrich, Ralf; Wagner-Kuhr, Jeannine; Wayand, Stefan; Weiler, Thomas; Zeise, Manuel; Daskalakis, Georgios; Geralis, Theodoros; Kesisoglou, Stilianos; Kyriakis, Aristotelis; Loukas, Demetrios; Manolakos, Ioannis; Markou, Athanasios; Markou, Christos; Mavrommatis, Charalampos; Ntomari, Eleni; Gouskos, Loukas; Mertzimekis, Theodoros; Panagiotou, Apostolos; Saoulidou, Niki; Evangelou, Ioannis; Foudas, Costas; Kokkas, Panagiotis; Manthos, Nikolaos; Papadopoulos, Ioannis; Patras, Vaios; Bencze, Gyorgy; Hajdu, Csaba; Hidas, Pàl; Horvath, Dezso; Sikler, Ferenc; Veszpremi, Viktor; Vesztergombi, Gyorgy; Beni, Noemi; Czellar, Sandor; Molnar, Jozsef; Palinkas, Jozsef; Szillasi, Zoltan; Karancsi, János; Raics, Peter; Trocsanyi, Zoltan Laszlo; Ujvari, Balazs; Beri, Suman Bala; Bhatnagar, Vipin; Dhingra, Nitish; Gupta, Ruchi; Kaur, Manjit; Mehta, Manuk Zubin; Nishu, Nishu; Saini, Lovedeep Kaur; Sharma, Archana; Singh, Jasbir; Kumar, Ashok; Kumar, Arun; Ahuja, Sudha; Bhardwaj, Ashutosh; Choudhary, Brajesh C; Malhotra, Shivali; Naimuddin, Md; Ranjan, Kirti; Sharma, Varun; Shivpuri, Ram Krishen; Banerjee, Sunanda; Bhattacharya, Satyaki; Dutta, Suchandra; Gomber, Bhawna; Jain, Sandhya; Jain, Shilpi; Khurana, Raman; Sarkar, Subir; Sharan, Manoj; Abdulsalam, Abdulla; Dutta, Dipanwita; Kailas, Swaminathan; Kumar, Vineet; Mohanty, Ajit Kumar; Pant, Lalit Mohan; Shukla, Prashant; Aziz, Tariq; Ganguly, Sanmay; Guchait, Monoranjan; Maity, Manas; Majumder, Gobinda; Mazumdar, Kajari; Mohanty, Gagan Bihari; Parida, Bibhuti; Sudhakar, Katta; Wickramage, Nadeesha; Banerjee, Sudeshna; Dugad, Shashikant; Arfaei, Hessamaddin; Bakhshiansohi, Hamed; Etesami, Seyed Mohsen; Fahim, Ali; Hashemi, Majid; Hesari, Hoda; Jafari, Abideh; Khakzad, Mohsen; Mohammadi Najafabadi, Mojtaba; Paktinat Mehdiabadi, Saeid; Safarzadeh, Batool; Zeinali, Maryam; Abbrescia, Marcello; Barbone, Lucia; Calabria, Cesare; Chhibra, Simranjit Singh; Colaleo, Anna; Creanza, Donato; De Filippis, Nicola; De Palma, Mauro; Fiore, Luigi; Iaselli, Giuseppe; Maggi, Giorgio; Maggi, Marcello; Marangelli, Bartolomeo; My, Salvatore; Nuzzo, Salvatore; Pacifico, Nicola; Pompili, Alexis; Pugliese, Gabriella; Selvaggi, Giovanna; Silvestris, Lucia; Singh, Gurpreet; Venditti, Rosamaria; Verwilligen, Piet; Zito, Giuseppe; Abbiendi, Giovanni; Benvenuti, Alberto; Bonacorsi, Daniele; Braibant-Giacomelli, Sylvie; Brigliadori, Luca; Capiluppi, Paolo; Castro, Andrea; Cavallo, Francesca Romana; Cuffiani, Marco; Dallavalle, Gaetano-Marco; Fabbri, Fabrizio; Fanfani, Alessandra; Fasanella, Daniele; Giacomelli, Paolo; Grandi, Claudio; Guiducci, Luigi; Marcellini, Stefano; Masetti, Gianni; Meneghelli, Marco; Montanari, Alessandro; Navarria, Francesco; Odorici, Fabrizio; Perrotta, Andrea; Primavera, Federica; Rossi, Antonio; Rovelli, Tiziano; Siroli, Gian Piero; Tosi, Nicolò; Travaglini, Riccardo; Albergo, Sebastiano; Cappello, Gigi; Chiorboli, Massimiliano; Costa, Salvatore; Potenza, Renato; Tricomi, Alessia; Tuve, Cristina; Barbagli, Giuseppe; Ciulli, Vitaliano; Civinini, Carlo; D'Alessandro, Raffaello; Focardi, Ettore; Frosali, Simone; Gallo, Elisabetta; Gonzi, Sandro; Meschini, Marco; Paoletti, Simone; Sguazzoni, Giacomo; Tropiano, Antonio; Benussi, Luigi; Bianco, Stefano; Colafranceschi, Stefano; Fabbri, Franco; Piccolo, Davide; Fabbricatore, Pasquale; Musenich, Riccardo; Tosi, Silvano; Benaglia, Andrea; De Guio, Federico; Di Matteo, Leonardo; Fiorendi, Sara; Gennai, Simone; Ghezzi, Alessio; Malvezzi, Sandra; Manzoni, Riccardo Andrea; Martelli, Arabella; Massironi, Andrea; Menasce, Dario; Moroni, Luigi; Paganoni, Marco; Pedrini, Daniele; Ragazzi, Stefano; Redaelli, Nicola; Sala, Silvano; Tabarelli de Fatis, Tommaso; Buontempo, Salvatore; Carrillo Montoya, Camilo Andres; Cavallo, Nicola; De Cosa, Annapaola; Dogangun, Oktay; Fabozzi, Francesco; Iorio, Alberto Orso Maria; Lista, Luca; Meola, Sabino; Merola, Mario; Paolucci, Pierluigi; Azzi, Patrizia; Bacchetta, Nicola; Bellan, Paolo; Bisello, Dario; Branca, Antonio; Carlin, Roberto; Checchia, Paolo; Dorigo, Tommaso; Dosselli, Umberto; Gasparini, Fabrizio; Gasparini, Ugo; Gozzelino, Andrea; Kanishchev, Konstantin; Lacaprara, Stefano; Lazzizzera, Ignazio; Margoni, Martino; Meneguzzo, Anna Teresa; Nespolo, Massimo; Pazzini, Jacopo; Ronchese, Paolo; Simonetto, Franco; Torassa, Ezio; Vanini, Sara; Zotto, Pierluigi; Zumerle, Gianni; Gabusi, Michele; Ratti, Sergio P; Riccardi, Cristina; Torre, Paola; Vitulo, Paolo; Biasini, Maurizio; Bilei, Gian Mario; Fanò, Livio; Lariccia, Paolo; Mantovani, Giancarlo; Menichelli, Mauro; Nappi, Aniello; Romeo, Francesco; Saha, Anirban; Santocchia, Attilio; Spiezia, Aniello; Taroni, Silvia; Azzurri, Paolo; Bagliesi, Giuseppe; Bernardini, Jacopo; Boccali, Tommaso; Broccolo, Giuseppe; Castaldi, Rino; D'Agnolo, Raffaele Tito; Dell'Orso, Roberto; Fiori, Francesco; Foà, Lorenzo; Giassi, Alessandro; Kraan, Aafke; Ligabue, Franco; Lomtadze, Teimuraz; Martini, Luca; Messineo, Alberto; Palla, Fabrizio; Rizzi, Andrea; Serban, Alin Titus; Spagnolo, Paolo; Squillacioti, Paola; Tenchini, Roberto; Tonelli, Guido; Venturi, Andrea; Verdini, Piero Giorgio; Barone, Luciano; Cavallari, Francesca; Del Re, Daniele; Diemoz, Marcella; Fanelli, Cristiano; Grassi, Marco; Longo, Egidio; Meridiani, Paolo; Micheli, Francesco; Nourbakhsh, Shervin; Organtini, Giovanni; Paramatti, Riccardo; Rahatlou, Shahram; Sigamani, Michael; Soffi, Livia; Amapane, Nicola; Arcidiacono, Roberta; Argiro, Stefano; Arneodo, Michele; Biino, Cristina; Cartiglia, Nicolo; Costa, Marco; Demaria, Natale; Mariotti, Chiara; Maselli, Silvia; Migliore, Ernesto; Monaco, Vincenzo; Musich, Marco; Obertino, Maria Margherita; Pastrone, Nadia; Pelliccioni, Mario; Potenza, Alberto; Romero, Alessandra; Ruspa, Marta; Sacchi, Roberto; Solano, Ada; Staiano, Amedeo; Belforte, Stefano; Candelise, Vieri; Casarsa, Massimo; Cossutti, Fabio; Della Ricca, Giuseppe; Gobbo, Benigno; Marone, Matteo; Montanino, Damiana; Penzo, Aldo; Schizzi, Andrea; Heo, Seong Gu; Kim, Tae Yeon; Nam, Soon-Kwon; Chang, Sunghyun; Kim, Dong Hee; Kim, Gui Nyun; Kong, Dae Jung; Park, Hyangkyu; Ro, Sang-Ryul; Son, Dong-Chul; Son, Taejin; Kim, Jae Yool; Kim, Zero Jaeho; Song, Sanghyeon; Choi, Suyong; Gyun, Dooyeon; Hong, Byung-Sik; Jo, Mihee; Kim, Hyunchul; Kim, Tae Jeong; Lee, Kyong Sei; Moon, Dong Ho; Park, Sung Keun; Choi, Minkyoo; Kim, Ji Hyun; Park, Chawon; Park, Inkyu; Park, Sangnam; Ryu, Geonmo; Cho, Yongjin; Choi, Young-Il; Choi, Young Kyu; Goh, Junghwan; Kim, Min Suk; Kwon, Eunhyang; Lee, Byounghoon; Lee, Jongseok; Lee, Sungeun; Seo, Hyunkwan; Yu, Intae; Bilinskas, Mykolas Jurgis; Grigelionis, Ignas; Janulis, Mindaugas; Juodagalvis, Andrius; Castilla-Valdez, Heriberto; De La Cruz-Burelo, Eduard; Heredia-de La Cruz, Ivan; Lopez-Fernandez, Ricardo; Magaña Villalba, Ricardo; Martínez-Ortega, Jorge; Sánchez Hernández, Alberto; Villasenor-Cendejas, Luis Manuel; Carrillo Moreno, Salvador; Vazquez Valencia, Fabiola; Salazar Ibarguen, Humberto Antonio; Casimiro Linares, Edgar; Morelos Pineda, Antonio; Reyes-Santos, Marco A; Krofcheck, David; Bell, Alan James; Butler, Philip H; Doesburg, Robert; Reucroft, Steve; Silverwood, Hamish; Ahmad, Muhammad; Asghar, Muhammad Irfan; Butt, Jamila; Hoorani, Hafeez R; Khalid, Shoaib; Khan, Wajid Ali; Khurshid, Taimoor; Qazi, Shamona; Shah, Mehar Ali; Shoaib, Muhammad; Bialkowska, Helena; Boimska, Bozena; Frueboes, Tomasz; Gokieli, Ryszard; Górski, Maciej; Kazana, Malgorzata; Nawrocki, Krzysztof; Romanowska-Rybinska, Katarzyna; Szleper, Michal; Wrochna, Grzegorz; Zalewski, Piotr; Brona, Grzegorz; Bunkowski, Karol; Cwiok, Mikolaj; Dominik, Wojciech; Doroba, Krzysztof; Kalinowski, Artur; Konecki, Marcin; Krolikowski, Jan; Almeida, Nuno; Bargassa, Pedrame; David Tinoco Mendes, Andre; Faccioli, Pietro; Ferreira Parracho, Pedro Guilherme; Gallinaro, Michele; Seixas, Joao; Varela, Joao; Vischia, Pietro; Belotelov, Ivan; Bunin, Pavel; Golutvin, Igor; Gorbunov, Ilya; Kamenev, Alexey; Karjavin, Vladimir; Kozlov, Guennady; Lanev, Alexander; Malakhov, Alexander; Moisenz, Petr; Palichik, Vladimir; Perelygin, Victor; Savina, Maria; Shmatov, Sergey; Smirnov, Vitaly; Volodko, Anton; Zarubin, Anatoli; Evstyukhin, Sergey; Golovtsov, Victor; Ivanov, Yury; Kim, Victor; Levchenko, Petr; Murzin, Victor; Oreshkin, Vadim; Smirnov, Igor; Sulimov, Valentin; Uvarov, Lev; Vavilov, Sergey; Vorobyev, Alexey; Vorobyev, Andrey; Andreev, Yuri; Dermenev, Alexander; Gninenko, Sergei; Golubev, Nikolai; Kirsanov, Mikhail; Krasnikov, Nikolai; Matveev, Viktor; Pashenkov, Anatoli; Tlisov, Danila; Toropin, Alexander; Epshteyn, Vladimir; Erofeeva, Maria; Gavrilov, Vladimir; Kossov, Mikhail; Lychkovskaya, Natalia; Popov, Vladimir; Safronov, Grigory; Semenov, Sergey; Stolin, Viatcheslav; Vlasov, Evgueni; Zhokin, Alexander; Belyaev, Andrey; Boos, Edouard; Dubinin, Mikhail; Dudko, Lev; Ershov, Alexander; Gribushin, Andrey; Klyukhin, Vyacheslav; Kodolova, Olga; Lokhtin, Igor; Markina, Anastasia; Obraztsov, Stepan; Perfilov, Maxim; Petrushanko, Sergey; Popov, Andrey; Sarycheva, Ludmila; Savrin, Viktor; Snigirev, Alexander; Andreev, Vladimir; Azarkin, Maksim; Dremin, Igor; Kirakosyan, Martin; Leonidov, Andrey; Mesyats, Gennady; Rusakov, Sergey V; Vinogradov, Alexey; Azhgirey, Igor; Bayshev, Igor; Bitioukov, Sergei; Grishin, Viatcheslav; Kachanov, Vassili; Konstantinov, Dmitri; Krychkine, Victor; Petrov, Vladimir; Ryutin, Roman; Sobol, Andrei; Tourtchanovitch, Leonid; Troshin, Sergey; Tyurin, Nikolay; Uzunian, Andrey; Volkov, Alexey; Adzic, Petar; Djordjevic, Milos; Ekmedzic, Marko; Krpic, Dragomir; Milosevic, Jovan; Aguilar-Benitez, Manuel; Alcaraz Maestre, Juan; Arce, Pedro; Battilana, Carlo; Calvo, Enrique; Cerrada, Marcos; Chamizo Llatas, Maria; Colino, Nicanor; De La Cruz, Begona; Delgado Peris, Antonio; Domínguez Vázquez, Daniel; Fernandez Bedoya, Cristina; Fernández Ramos, Juan Pablo; Ferrando, Antonio; Flix, Jose; Fouz, Maria Cruz; Garcia-Abia, Pablo; Gonzalez Lopez, Oscar; Goy Lopez, Silvia; Hernandez, Jose M; Josa, Maria Isabel; Merino, Gonzalo; Puerta Pelayo, Jesus; Quintario Olmeda, Adrián; Redondo, Ignacio; Romero, Luciano; Santaolalla, Javier; Senghi Soares, Mara; Willmott, Carlos; Albajar, Carmen; Codispoti, Giuseppe; de Trocóniz, Jorge F; Brun, Hugues; Cuevas, Javier; Fernandez Menendez, Javier; Folgueras, Santiago; Gonzalez Caballero, Isidro; Lloret Iglesias, Lara; Piedra Gomez, Jonatan; Brochero Cifuentes, Javier Andres; Cabrillo, Iban Jose; Calderon, Alicia; Chuang, Shan-Huei; Duarte Campderros, Jordi; Felcini, Marta; Fernandez, Marcos; Gomez, Gervasio; Gonzalez Sanchez, Javier; Graziano, Alberto; Jorda, Clara; Lopez Virto, Amparo; Marco, Jesus; Marco, Rafael; Martinez Rivero, Celso; Matorras, Francisco; Munoz Sanchez, Francisca Javiela; Rodrigo, Teresa; Rodríguez-Marrero, Ana Yaiza; Ruiz-Jimeno, Alberto; Scodellaro, Luca; Vila, Ivan; Vilar Cortabitarte, Rocio; Abbaneo, Duccio; Auffray, Etiennette; Auzinger, Georg; Bachtis, Michail; Baillon, Paul; Ball, Austin; Barney, David; Benitez, Jose F; Bernet, Colin; Bianchi, Giovanni; Bloch, Philippe; Bocci, Andrea; Bonato, Alessio; Botta, Cristina; Breuker, Horst; Camporesi, Tiziano; Cerminara, Gianluca; Christiansen, Tim; Coarasa Perez, Jose Antonio; D'Enterria, David; Dabrowski, Anne; De Roeck, Albert; Di Guida, Salvatore; Dobson, Marc; Dupont-Sagorin, Niels; Elliott-Peisert, Anna; Frisch, Benjamin; Funk, Wolfgang; Georgiou, Georgios; Giffels, Manuel; Gigi, Dominique; Gill, Karl; Giordano, Domenico; Girone, Maria; Giunta, Marina; Glege, Frank; Gomez-Reino Garrido, Robert; Govoni, Pietro; Gowdy, Stephen; Guida, Roberto; Hansen, Magnus; Harris, Philip; Hartl, Christian; Harvey, John; Hegner, Benedikt; Hinzmann, Andreas; Innocente, Vincenzo; Janot, Patrick; Kaadze, Ketino; Karavakis, Edward; Kousouris, Konstantinos; Lecoq, Paul; Lee, Yen-Jie; Lenzi, Piergiulio; Lourenco, Carlos; Magini, Nicolo; Maki, Tuula; Malberti, Martina; Malgeri, Luca; Mannelli, Marcello; Masetti, Lorenzo; Meijers, Frans; Mersi, Stefano; Meschi, Emilio; Moser, Roland; Mozer, Matthias Ulrich; Mulders, Martijn; Musella, Pasquale; Nesvold, Erik; Orimoto, Toyoko; Orsini, Luciano; Palencia Cortezon, Enrique; Perez, Emmanuelle; Perrozzi, Luca; Petrilli, Achille; Pfeiffer, Andreas; Pierini, Maurizio; Pimiä, Martti; Piparo, Danilo; Polese, Giovanni; Quertenmont, Loic; Racz, Attila; Reece, William; Rodrigues Antunes, Joao; Rolandi, Gigi; Rovelli, Chiara; Rovere, Marco; Sakulin, Hannes; Santanastasio, Francesco; Schäfer, Christoph; Schwick, Christoph; Segoni, Ilaria; Sekmen, Sezen; Sharma, Archana; Siegrist, Patrice; Silva, Pedro; Simon, Michal; Sphicas, Paraskevas; Spiga, Daniele; Tsirou, Andromachi; Veres, Gabor Istvan; Vlimant, Jean-Roch; Wöhri, Hermine Katharina; Worm, Steven; Zeuner, Wolfram Dietrich; Bertl, Willi; Deiters, Konrad; Erdmann, Wolfram; Gabathuler, Kurt; Horisberger, Roland; Ingram, Quentin; Kaestli, Hans-Christian; König, Stefan; Kotlinski, Danek; Langenegger, Urs; Meier, Frank; Renker, Dieter; Rohe, Tilman; Bäni, Lukas; Bortignon, Pierluigi; Buchmann, Marco-Andrea; Casal, Bruno; Chanon, Nicolas; Deisher, Amanda; Dissertori, Günther; Dittmar, Michael; Donegà, Mauro; Dünser, Marc; Eugster, Jürg; Freudenreich, Klaus; Grab, Christoph; Hits, Dmitry; Lecomte, Pierre; Lustermann, Werner; Marini, Andrea Carlo; Martinez Ruiz del Arbol, Pablo; Mohr, Niklas; Moortgat, Filip; Nägeli, Christoph; Nef, Pascal; Nessi-Tedaldi, Francesca; Pandolfi, Francesco; Pape, Luc; Pauss, Felicitas; Peruzzi, Marco; Ronga, Frederic Jean; Rossini, Marco; Sala, Leonardo; Sanchez, Ann - Karin; Starodumov, Andrei; Stieger, Benjamin; Takahashi, Maiko; Tauscher, Ludwig; Thea, Alessandro; Theofilatos, Konstantinos; Treille, Daniel; Urscheler, Christina; Wallny, Rainer; Weber, Hannsjoerg Artur; Wehrli, Lukas; Amsler, Claude; Chiochia, Vincenzo; De Visscher, Simon; Favaro, Carlotta; Ivova Rikova, Mirena; Millan Mejias, Barbara; Otiougova, Polina; Robmann, Peter; Snoek, Hella; Tupputi, Salvatore; Verzetti, Mauro; Chang, Yuan-Hann; Chen, Kuan-Hsin; Kuo, Chia-Ming; Li, Syue-Wei; Lin, Willis; Lu, Yun-Ju; Mekterovic, Darko; Singh, Anil; Volpe, Roberta; Yu, Shin-Shan; Bartalini, Paolo; Chang, Paoti; Chang, You-Hao; Chang, Yu-Wei; Chao, Yuan; Chen, Kai-Feng; Dietz, Charles; Grundler, Ulysses; Hou, George Wei-Shu; Hsiung, Yee; Kao, Kai-Yi; Lei, Yeong-Jyi; Lu, Rong-Shyang; Majumder, Devdatta; Petrakou, Eleni; Shi, Xin; Shiu, Jing-Ge; Tzeng, Yeng-Ming; Wan, Xia; Wang, Minzu; Asavapibhop, Burin; Srimanobhas, Norraphat; Adiguzel, Aytul; Bakirci, Mustafa Numan; Cerci, Salim; Dozen, Candan; Dumanoglu, Isa; Eskut, Eda; Girgis, Semiray; Gokbulut, Gul; Gurpinar, Emine; Hos, Ilknur; Kangal, Evrim Ersin; Karaman, Turker; Karapinar, Guler; Kayis Topaksu, Aysel; Onengut, Gulsen; Ozdemir, Kadri; Ozturk, Sertac; Polatoz, Ayse; Sogut, Kenan; Sunar Cerci, Deniz; Tali, Bayram; Topakli, Huseyin; Vergili, Latife Nukhet; Vergili, Mehmet; Akin, Ilina Vasileva; Aliev, Takhmasib; Bilin, Bugra; Bilmis, Selcuk; Deniz, Muhammed; Gamsizkan, Halil; Guler, Ali Murat; Ocalan, Kadir; Ozpineci, Altug; Serin, Meltem; Sever, Ramazan; Surat, Ugur Emrah; Yalvac, Metin; Yildirim, Eda; Zeyrek, Mehmet; Gülmez, Erhan; Isildak, Bora; Kaya, Mithat; Kaya, Ozlem; Ozkorucuklu, Suat; Sonmez, Nasuf; Cankocak, Kerem; Levchuk, Leonid; Brooke, James John; Clement, Emyr; Cussans, David; Flacher, Henning; Frazier, Robert; Goldstein, Joel; Grimes, Mark; Heath, Greg P; Heath, Helen F; Kreczko, Lukasz; Metson, Simon; Newbold, Dave M; Nirunpong, Kachanon; Poll, Anthony; Senkin, Sergey; Smith, Vincent J; Williams, Thomas; Basso, Lorenzo; Bell, Ken W; Belyaev, Alexander; Brew, Christopher; Brown, Robert M; Cockerill, David JA; Coughlan, John A; Harder, Kristian; Harper, Sam; Jackson, James; Kennedy, Bruce W; Olaiya, Emmanuel; Petyt, David; Radburn-Smith, Benjamin Charles; Shepherd-Themistocleous, Claire; Tomalin, Ian R; Womersley, William John; Bainbridge, Robert; Ball, Gordon; Beuselinck, Raymond; Buchmuller, Oliver; Colling, David; Cripps, Nicholas; Cutajar, Michael; Dauncey, Paul; Davies, Gavin; Della Negra, Michel; Ferguson, William; Fulcher, Jonathan; Futyan, David; Gilbert, Andrew; Guneratne Bryer, Arlo; Hall, Geoffrey; Hatherell, Zoe; Hays, Jonathan; Iles, Gregory; Jarvis, Martyn; Karapostoli, Georgia; Lyons, Louis; Magnan, Anne-Marie; Marrouche, Jad; Mathias, Bryn; Nandi, Robin; Nash, Jordan; Nikitenko, Alexander; Papageorgiou, Anastasios; Pela, Joao; Pesaresi, Mark; Petridis, Konstantinos; Pioppi, Michele; Raymond, David Mark; Rogerson, Samuel; Rose, Andrew; Ryan, Matthew John; Seez, Christopher; Sharp, Peter; Sparrow, Alex; Stoye, Markus; Tapper, Alexander; Vazquez Acosta, Monica; Virdee, Tejinder; Wakefield, Stuart; Wardle, Nicholas; Whyntie, Tom; Chadwick, Matthew; Cole, Joanne; Hobson, Peter R; Khan, Akram; Kyberd, Paul; Leggat, Duncan; Leslie, Dawn; Martin, William; Reid, Ivan; Symonds, Philip; Teodorescu, Liliana; Turner, Mark; Hatakeyama, Kenichi; Liu, Hongxuan; Scarborough, Tara; Charaf, Otman; Henderson, Conor; Rumerio, Paolo; Avetisyan, Aram; Bose, Tulika; Fantasia, Cory; Heister, Arno; St John, Jason; Lawson, Philip; Lazic, Dragoslav; Rohlf, James; Sperka, David; Sulak, Lawrence; Alimena, Juliette; Bhattacharya, Saptaparna; Cutts, David; Demiragli, Zeynep; Ferapontov, Alexey; Garabedian, Alex; Heintz, Ulrich; Jabeen, Shabnam; Kukartsev, Gennadiy; Laird, Edward; Landsberg, Greg; Luk, Michael; Narain, Meenakshi; Nguyen, Duong; Segala, Michael; Sinthuprasith, Tutanon; Speer, Thomas; Breedon, Richard; Breto, Guillermo; Calderon De La Barca Sanchez, Manuel; Chauhan, Sushil; Chertok, Maxwell; Conway, John; Conway, Rylan; Cox, Peter Timothy; Dolen, James; Erbacher, Robin; Gardner, Michael; Houtz, Rachel; Ko, Winston; Kopecky, Alexandra; Lander, Richard; Mall, Orpheus; Miceli, Tia; Pellett, Dave; Ricci-Tam, Francesca; Rutherford, Britney; Searle, Matthew; Smith, John; Squires, Michael; Tripathi, Mani; Vasquez Sierra, Ricardo; Yohay, Rachel; Andreev, Valeri; Cline, David; Cousins, Robert; Duris, Joseph; Erhan, Samim; Everaerts, Pieter; Farrell, Chris; Hauser, Jay; Ignatenko, Mikhail; Jarvis, Chad; Plager, Charles; Rakness, Gregory; Schlein, Peter; Traczyk, Piotr; Valuev, Vyacheslav; Weber, Matthias; Babb, John; Clare, Robert; Dinardo, Mauro Emanuele; Ellison, John Anthony; Gary, J William; Giordano, Ferdinando; Hanson, Gail; Jeng, Geng-Yuan; Liu, Hongliang; Long, Owen Rosser; Luthra, Arun; Nguyen, Harold; Paramesvaran, Sudarshan; Sturdy, Jared; Sumowidagdo, Suharyo; Wilken, Rachel; Wimpenny, Stephen; Andrews, Warren; Branson, James G; Cerati, Giuseppe Benedetto; Cittolin, Sergio; Evans, David; Golf, Frank; Holzner, André; Kelley, Ryan; Lebourgeois, Matthew; Letts, James; Macneill, Ian; Mangano, Boris; Padhi, Sanjay; Palmer, Christopher; Petrucciani, Giovanni; Pieri, Marco; Sani, Matteo; Sharma, Vivek; Simon, Sean; Sudano, Elizabeth; Tadel, Matevz; Tu, Yanjun; Vartak, Adish; Wasserbaech, Steven; Würthwein, Frank; Yagil, Avraham; Yoo, Jaehyeok; Barge, Derek; Bellan, Riccardo; Campagnari, Claudio; D'Alfonso, Mariarosaria; Danielson, Thomas; Flowers, Kristen; Geffert, Paul; Incandela, Joe; Justus, Christopher; Kalavase, Puneeth; Kovalskyi, Dmytro; Krutelyov, Vyacheslav; Lowette, Steven; Mccoll, Nickolas; Pavlunin, Viktor; Rebassoo, Finn; Ribnik, Jacob; Richman, Jeffrey; Rossin, Roberto; Stuart, David; To, Wing; West, Christopher; Apresyan, Artur; Bornheim, Adolf; Chen, Yi; Di Marco, Emanuele; Duarte, Javier; Gataullin, Marat; Ma, Yousi; Mott, Alexander; Newman, Harvey B; Rogan, Christopher; Spiropulu, Maria; Timciuc, Vladlen; Veverka, Jan; Wilkinson, Richard; Xie, Si; Yang, Yong; Zhu, Ren-Yuan; Akgun, Bora; Azzolini, Virginia; Calamba, Aristotle; Carroll, Ryan; Ferguson, Thomas; Iiyama, Yutaro; Jang, Dong Wook; Liu, Yueh-Feng; Paulini, Manfred; Vogel, Helmut; Vorobiev, Igor; Cumalat, John Perry; Drell, Brian Robert; Ford, William T; Gaz, Alessandro; Luiggi Lopez, Eduardo; Smith, James; Stenson, Kevin; Ulmer, Keith; Wagner, Stephen Robert; Alexander, James; Chatterjee, Avishek; Eggert, Nicholas; Gibbons, Lawrence Kent; Heltsley, Brian; Khukhunaishvili, Aleko; Kreis, Benjamin; Mirman, Nathan; Nicolas Kaufman, Gala; Patterson, Juliet Ritchie; Ryd, Anders; Salvati, Emmanuele; Sun, Werner; Teo, Wee Don; Thom, Julia; Thompson, Joshua; Tucker, Jordan; Vaughan, Jennifer; Weng, Yao; Winstrom, Lucas; Wittich, Peter; Winn, Dave; Abdullin, Salavat; Albrow, Michael; Anderson, Jacob; Apollinari, Giorgio; Bauerdick, Lothar AT; Beretvas, Andrew; Berryhill, Jeffrey; Bhat, Pushpalatha C; Bloch, Ingo; Burkett, Kevin; Butler, Joel Nathan; Chetluru, Vasundhara; Cheung, Harry; Chlebana, Frank; Elvira, Victor Daniel; Fisk, Ian; Freeman, Jim; Gao, Yanyan; Green, Dan; Gutsche, Oliver; Hanlon, Jim; Harris, Robert M; Hirschauer, James; Hooberman, Benjamin; Jindariani, Sergo; Johnson, Marvin; Joshi, Umesh; Kilminster, Benjamin; Klima, Boaz; Kunori, Shuichi; Kwan, Simon; Leonidopoulos, Christos; Linacre, Jacob; Lincoln, Don; Lipton, Ron; Lykken, Joseph; Maeshima, Kaori; Marraffino, John Michael; Maruyama, Sho; Mason, David; McBride, Patricia; Mishra, Kalanand; Mrenna, Stephen; Musienko, Yuri; Newman-Holmes, Catherine; O'Dell, Vivian; Sexton-Kennedy, Elizabeth; Sharma, Seema; Spalding, William J; Spiegel, Leonard; Taylor, Lucas; Tkaczyk, Slawek; Tran, Nhan Viet; Uplegger, Lorenzo; Vaandering, Eric Wayne; Vidal, Richard; Whitmore, Juliana; Wu, Weimin; Yang, Fan; Yun, Jae Chul; Acosta, Darin; Avery, Paul; Bourilkov, Dimitri; Chen, Mingshui; Cheng, Tongguang; Das, Souvik; De Gruttola, Michele; Di Giovanni, Gian Piero; Dobur, Didar; Drozdetskiy, Alexey; Field, Richard D; Fisher, Matthew; Fu, Yu; Furic, Ivan-Kresimir; Gartner, Joseph; Hugon, Justin; Kim, Bockjoo; Konigsberg, Jacobo; Korytov, Andrey; Kropivnitskaya, Anna; Kypreos, Theodore; Low, Jia Fu; Matchev, Konstantin; Milenovic, Predrag; Mitselmakher, Guenakh; Muniz, Lana; Park, Myeonghun; Remington, Ronald; Rinkevicius, Aurelijus; Sellers, Paul; Skhirtladze, Nikoloz; Snowball, Matthew; Yelton, John; Zakaria, Mohammed; Gaultney, Vanessa; Hewamanage, Samantha; Lebolo, Luis Miguel; Linn, Stephan; Markowitz, Pete; Martinez, German; Rodriguez, Jorge Luis; Adams, Todd; Askew, Andrew; Bochenek, Joseph; Chen, Jie; Diamond, Brendan; Gleyzer, Sergei V; Haas, Jeff; Hagopian, Sharon; Hagopian, Vasken; Jenkins, Merrill; Johnson, Kurtis F; Prosper, Harrison; Veeraraghavan, Venkatesh; Weinberg, Marc; Baarmand, Marc M; Dorney, Brian; Hohlmann, Marcus; Kalakhety, Himali; Vodopiyanov, Igor; Yumiceva, Francisco; Adams, Mark Raymond; Anghel, Ioana Maria; Apanasevich, Leonard; Bai, Yuting; Bazterra, Victor Eduardo; Betts, Russell Richard; Bucinskaite, Inga; Callner, Jeremy; Cavanaugh, Richard; Evdokimov, Olga; Gauthier, Lucie; Gerber, Cecilia Elena; Hofman, David Jonathan; Khalatyan, Samvel; Lacroix, Florent; Malek, Magdalena; O'Brien, Christine; Silkworth, Christopher; Strom, Derek; Turner, Paul; Varelas, Nikos; Akgun, Ugur; Albayrak, Elif Asli; Bilki, Burak; Clarida, Warren; Duru, Firdevs; Merlo, Jean-Pierre; Mermerkaya, Hamit; Mestvirishvili, Alexi; Moeller, Anthony; Nachtman, Jane; Newsom, Charles Ray; Norbeck, Edwin; Onel, Yasar; Ozok, Ferhat; Sen, Sercan; Tan, Ping; Tiras, Emrah; Wetzel, James; Yetkin, Taylan; Yi, Kai; Barnett, Bruce Arnold; Blumenfeld, Barry; Bolognesi, Sara; Fehling, David; Giurgiu, Gavril; Gritsan, Andrei; Guo, Zijin; Hu, Guofan; Maksimovic, Petar; Rappoccio, Salvatore; Swartz, Morris; Whitbeck, Andrew; Baringer, Philip; Bean, Alice; Benelli, Gabriele; Kenny Iii, Raymond Patrick; Murray, Michael; Noonan, Daniel; Sanders, Stephen; Stringer, Robert; Tinti, Gemma; Wood, Jeffrey Scott; Zhukova, Victoria; Barfuss, Anne-Fleur; Bolton, Tim; Chakaberia, Irakli; Ivanov, Andrew; Khalil, Sadia; Makouski, Mikhail; Maravin, Yurii; Shrestha, Shruti; Svintradze, Irakli; Gronberg, Jeffrey; Lange, David; Wright, Douglas; Baden, Drew; Boutemeur, Madjid; Calvert, Brian; Eno, Sarah Catherine; Gomez, Jaime; Hadley, Nicholas John; Kellogg, Richard G; Kirn, Malina; Kolberg, Ted; Lu, Ying; Marionneau, Matthieu; Mignerey, Alice; Pedro, Kevin; Skuja, Andris; Temple, Jeffrey; Tonjes, Marguerite; Tonwar, Suresh C; Twedt, Elizabeth; Apyan, Aram; Bauer, Gerry; Bendavid, Joshua; Busza, Wit; Butz, Erik; Cali, Ivan Amos; Chan, Matthew; Dutta, Valentina; Gomez Ceballos, Guillelmo; Goncharov, Maxim; Hahn, Kristan Allan; Kim, Yongsun; Klute, Markus; Krajczar, Krisztian; Luckey, Paul David; Ma, Teng; Nahn, Steve; Paus, Christoph; Ralph, Duncan; Roland, Christof; Roland, Gunther; Rudolph, Matthew; Stephans, George; Stöckli, Fabian; Sumorok, Konstanty; Sung, Kevin; Velicanu, Dragos; Wenger, Edward Allen; Wolf, Roger; Wyslouch, Bolek; Yang, Mingming; Yilmaz, Yetkin; Yoon, Sungho; Zanetti, Marco; Cooper, Seth; Dahmes, Bryan; De Benedetti, Abraham; Franzoni, Giovanni; Gude, Alexander; Kao, Shih-Chuan; Klapoetke, Kevin; Kubota, Yuichi; Mans, Jeremy; Pastika, Nathaniel; Rusack, Roger; Sasseville, Michael; Singovsky, Alexander; Tambe, Norbert; Turkewitz, Jared; Cremaldi, Lucien Marcus; Kroeger, Rob; Perera, Lalith; Rahmat, Rahmat; Sanders, David A; Avdeeva, Ekaterina; Bloom, Kenneth; Bose, Suvadeep; Claes, Daniel R; Dominguez, Aaron; Eads, Michael; Keller, Jason; Kravchenko, Ilya; Lazo-Flores, Jose; Malbouisson, Helena; Malik, Sudhir; Snow, Gregory R; Godshalk, Andrew; Iashvili, Ia; Jain, Supriya; Kharchilava, Avto; Kumar, Ashish; Alverson, George; Barberis, Emanuela; Baumgartel, Darin; Chasco, Matthew; Haley, Joseph; Nash, David; Trocino, Daniele; Wood, Darien; Zhang, Jinzhong; Anastassov, Anton; Kubik, Andrew; Lusito, Letizia; Mucia, Nicholas; Odell, Nathaniel; Ofierzynski, Radoslaw Adrian; Pollack, Brian; Pozdnyakov, Andrey; Schmitt, Michael Henry; Stoynev, Stoyan; Velasco, Mayda; Won, Steven; Antonelli, Louis; Berry, Douglas; Brinkerhoff, Andrew; Chan, Kwok Ming; Hildreth, Michael; Jessop, Colin; Karmgard, Daniel John; Kolb, Jeff; Lannon, Kevin; Luo, Wuming; Lynch, Sean; Marinelli, Nancy; Morse, David Michael; Pearson, Tessa; Planer, Michael; Ruchti, Randy; Slaunwhite, Jason; Valls, Nil; Wayne, Mitchell; Wolf, Matthias; Bylsma, Ben; Durkin, Lloyd Stanley; Hill, Christopher; Hughes, Richard; Kotov, Khristian; Ling, Ta-Yung; Puigh, Darren; Rodenburg, Marissa; Vuosalo, Carl; Williams, Grayson; Winer, Brian L; Berry, Edmund; Elmer, Peter; Halyo, Valerie; Hebda, Philip; Hegeman, Jeroen; Hunt, Adam; Jindal, Pratima; Koay, Sue Ann; Lopes Pegna, David; Lujan, Paul; Marlow, Daniel; Medvedeva, Tatiana; Mooney, Michael; Olsen, James; Piroué, Pierre; Quan, Xiaohang; Raval, Amita; Saka, Halil; Stickland, David; Tully, Christopher; Werner, Jeremy Scott; Zuranski, Andrzej; Brownson, Eric; Lopez, Angel; Mendez, Hector; Ramirez Vargas, Juan Eduardo; Alagoz, Enver; Barnes, Virgil E; Benedetti, Daniele; Bolla, Gino; Bortoletto, Daniela; De Mattia, Marco; Everett, Adam; Hu, Zhen; Jones, Matthew; Koybasi, Ozhan; Kress, Matthew; Laasanen, Alvin T; Leonardo, Nuno; Maroussov, Vassili; Merkel, Petra; Miller, David Harry; Neumeister, Norbert; Shipsey, Ian; Silvers, David; Svyatkovskiy, Alexey; Vidal Marono, Miguel; Yoo, Hwi Dong; Zablocki, Jakub; Zheng, Yu; Guragain, Samir; Parashar, Neeti; Adair, Antony; Boulahouache, Chaouki; Ecklund, Karl Matthew; Geurts, Frank JM; Li, Wei; Padley, Brian Paul; Redjimi, Radia; Roberts, Jay; Zabel, James; Betchart, Burton; Bodek, Arie; Chung, Yeon Sei; Covarelli, Roberto; de Barbaro, Pawel; Demina, Regina; Eshaq, Yossof; Ferbel, Thomas; Garcia-Bellido, Aran; Goldenzweig, Pablo; Han, Jiyeon; Harel, Amnon; Miner, Daniel Carl; Vishnevskiy, Dmitry; Zielinski, Marek; Bhatti, Anwar; Ciesielski, Robert; Demortier, Luc; Goulianos, Konstantin; Lungu, Gheorghe; Malik, Sarah; Mesropian, Christina; Arora, Sanjay; Barker, Anthony; Chou, John Paul; Contreras-Campana, Christian; Contreras-Campana, Emmanuel; Duggan, Daniel; Ferencek, Dinko; Gershtein, Yuri; Gray, Richard; Halkiadakis, Eva; Hidas, Dean; Lath, Amitabh; Panwalkar, Shruti; Park, Michael; Patel, Rishi; Rekovic, Vladimir; Robles, Jorge; Rose, Keith; Salur, Sevil; Schnetzer, Steve; Seitz, Claudia; Somalwar, Sunil; Stone, Robert; Thomas, Scott; Walker, Matthew; Cerizza, Giordano; Hollingsworth, Matthew; Spanier, Stefan; Yang, Zong-Chang; York, Andrew; Eusebi, Ricardo; Flanagan, Will; Gilmore, Jason; Kamon, Teruki; Khotilovich, Vadim; Montalvo, Roy; Osipenkov, Ilya; Pakhotin, Yuriy; Perloff, Alexx; Roe, Jeffrey; Safonov, Alexei; Sakuma, Tai; Sengupta, Sinjini; Suarez, Indara; Tatarinov, Aysen; Toback, David; Akchurin, Nural; Damgov, Jordan; Dragoiu, Cosmin; Dudero, Phillip Russell; Jeong, Chiyoung; Kovitanggoon, Kittikul; Lee, Sung Won; Libeiro, Terence; Roh, Youn; Volobouev, Igor; Appelt, Eric; Delannoy, Andrés G; Florez, Carlos; Greene, Senta; Gurrola, Alfredo; Johns, Willard; Kurt, Pelin; Maguire, Charles; Melo, Andrew; Sharma, Monika; Sheldon, Paul; Snook, Benjamin; Tuo, Shengquan; Velkovska, Julia; Arenton, Michael Wayne; Balazs, Michael; Boutle, Sarah; Cox, Bradley; Francis, Brian; Goodell, Joseph; Hirosky, Robert; Ledovskoy, Alexander; Lin, Chuanzhe; Neu, Christopher; Wood, John; Gollapinni, Sowjanya; Harr, Robert; Karchin, Paul Edmund; Kottachchi Kankanamge Don, Chamath; Lamichhane, Pramod; Sakharov, Alexandre; Anderson, Michael; Belknap, Donald; Borrello, Laura; Carlsmith, Duncan; Cepeda, Maria; Dasu, Sridhara; Friis, Evan; Gray, Lindsey; Grogg, Kira Suzanne; Grothe, Monika; Hall-Wilton, Richard; Herndon, Matthew; Hervé, Alain; Klabbers, Pamela; Klukas, Jeffrey; Lanaro, Armando; Lazaridis, Christos; Leonard, Jessica; Loveless, Richard; Mohapatra, Ajit; Ojalvo, Isabel; Palmonari, Francesco; Pierro, Giuseppe Antonio; Ross, Ian; Savin, Alexander; Smith, Wesley H; Swanson, Joshua

    2013-01-09

    A measurement is presented of the ZZ production cross section in the ZZ to 2l 2l' decay mode with l = e, mu and l' = e, mu, tau in proton-proton collisions at $\\sqrt{s}$ = 7 TeV with the CMS experiment at the LHC. Results are based on data corresponding to an integrated luminosity of 5.0 inverse femtobarns. The measured cross section sigma(pp to ZZ) = 6.24 [+0.86/-0.80] (stat.) [+0.41/-0.32] (syst.) +/- 0.14 (lumi.) pb is consistent with the standard model predictions. The following limits on ZZZ and ZZ gamma anomalous trilinear gauge couplings are set at 95% confidence level: -0.011$\\lt$ f[4;Z] $\\lt$ 0.012, -0.012 $\\lt$ f[5;Z] $\\lt$ 0.012, -0.013 $\\lt$ f[4;gamma]<0.015, and -0.014 $\\lt$ f[5,gamma] $\\lt$ 0.014.

  3. Chimeric L2-Based Virus-Like Particle (VLP Vaccines Targeting Cutaneous Human Papillomaviruses (HPV.

    Directory of Open Access Journals (Sweden)

    Bettina Huber

    Full Text Available Common cutaneous human papillomavirus (HPV types induce skin warts, whereas species beta HPV are implicated, together with UV-radiation, in the development of non-melanoma skin cancer (NMSC in immunosuppressed patients. Licensed HPV vaccines contain virus-like particles (VLP self-assembled from L1 major capsid proteins that provide type-restricted protection against mucosal HPV infections causing cervical and other ano-genital and oro-pharyngeal carcinomas and warts (condylomas, but do not target heterologous HPV. Experimental papillomavirus vaccines have been designed based on L2 minor capsid proteins that contain type-common neutralization epitopes, to broaden protection to heterologous mucosal and cutaneous HPV types. Repetitive display of the HPV16 L2 cross-neutralization epitope RG1 (amino acids (aa 17-36 on the surface of HPV16 L1 VLP has greatly enhanced immunogenicity of the L2 peptide. To more directly target cutaneous HPV, L1 fusion proteins were designed that incorporate the RG1 homolog of beta HPV17, the beta HPV5 L2 peptide aa53-72, or the common cutaneous HPV4 RG1 homolog, inserted into DE surface loops of HPV1, 5, 16 or 18 L1 VLP scaffolds. Baculovirus expressed chimeric proteins self-assembled into VLP and VLP-raised NZW rabbit immune sera were evaluated by ELISA and L1- and L2-based pseudovirion (PsV neutralizing assays, including 12 novel beta PsV types. Chimeric VLP displaying the HPV17 RG1 epitope, but not the HPV5L2 aa53-72 epitope, induced cross-neutralizing humoral immune responses to beta HPV. In vivo cross-protection was evaluated by passive serum transfer in a murine PsV challenge model. Immune sera to HPV16L1-17RG1 VLP (cross- protected against beta HPV5/20/24/38/96/16 (but not type 76, while antisera to HPV5L1-17RG1 VLP cross-protected against HPV20/24/96 only, and sera to HPV1L1-4RG1 VLP cross-protected against HPV4 challenge. In conclusion, RG1-based VLP are promising next generation vaccine candidates to target

  4. Cloning and expression of ligand-gated ion-channel receptor L2 in central nervous system

    International Nuclear Information System (INIS)

    Houtani, Takeshi; Munemoto, Yumi; Kase, Masahiko; Sakuma, Satoru; Tsutsumi, Toshiyuki; Sugimoto, Tetsuo

    2005-01-01

    An orphan receptor of ligand-gated ion-channel type (L2, also termed ZAC according to the presence of zinc ion for channel activation) was identified by computer-assisted search programs on human genome database. The L2 protein shares partial homology with serotonin receptors 5HT3A and 5HT3B. We have cloned L2 cDNA derived from human caudate nucleus and characterized the exon-intron structure as follows: (1) The L2 protein has four transmembrane regions (M1-M4) and a long cytoplasmic loop between M3 and M4. (2) The sequence is conserved in species including chimpanzee, dog, cow, and opossum. (3) Nine exons form its protein-coding region and especially exon 5 corresponds to a disulfide bond region on the amino-terminal side. Our analysis using multiple tissue cDNA panels revealed that at least two splicing variants of L2 mRNA are present. The cDNA PCR amplification study revealed that L2 mRNA is expressed in tissues including brain, pancreas, liver, lung, heart, kidney, and skeletal muscle while 5HT3A mRNA could be detected in brain, heart, placenta, lung, kidney, pancreas, and skeletal muscle, and 5HT3B mRNA in brain, kidney, and skeletal muscle, suggesting different significance in tissue expression of these receptors. Regional expression of L2 mRNA and protein was examined in brain. The RT-PCR studies confirmed L2 mRNA expression in hippocampus, striatum, amygdala, and thalamus in adult brain. The L2 protein was immunolocalized by using antipeptide antibodies. Immunostained tissue sections revealed that L2-like immunoreactivity was dominantly expressed in the hippocampal CA3 pyramidal cells and in the polymorphic layer of the dentate gyrus. We analyzed the expression of L2 protein in HEK293 cells using GFP fusion protein reporter system. Western blots revealed that L2 protein confers sugar chains on the extracellular side. In transfected HEK293 cells, cellular membranes and intracellular puncta were densely labeled with GFP, suggesting selective dispatch to the

  5. Subcooled decompression analysis of the ROSA and the LOFT semiscale blowdown test data with the digital computer code DEPCO-MULTI

    International Nuclear Information System (INIS)

    Namatame, Ken; Kobayashi, Kensuke

    1975-12-01

    In the ROSA (Rig of Safety Assessment) program, the digital computer code DEPCO-SINGLE and DEPCO-MULTI (Subcooled Decompression Process in Loss-of-Coolant Accident - Single Pipe and - Multiple Pipe Network) were prepared to study thermo-hydraulic behavior of the primary coolant in subcooled decompression of the PWR LOCA. The analytical results with DEPCO-MULTI on the subcooled decompression phenomena are presented for ROSA-I, ROSA-II and LOFT 500, 600, 700 and 800 series experiments. The effects of space mesh length, elasticity of pressure boundary materials and simplification for computational piping system on the computed result are described. This will be the final work on the study of the subcooled decompression analysis as for the ROSA program, and the authors wish that the present code shall further be examined with the data of much advanced experiments. (auth.)

  6. Hexagonal perovskites with cationic vacancies. 25. Hexagonal 5 L stacking polytypes in the systems Ba/sub 5/BaWsub(3-x)sup(VI)Msub(x)sup(V)vacantOsub(15-x/2)vacantsub(x/2) with Msup(V) = Nb, Ta

    Energy Technology Data Exchange (ETDEWEB)

    Kemmler-Sack, S [Tuebingen Univ. (Germany, F.R.). Lehrstuhl fuer Anorganische Chemie 2

    1980-12-01

    In the systems BaO-M/sub 2/sup(V)O/sub 5/-WO/sub 3/ (Msup(V) = Nb, Ta) a new phase Ba/sub 5/BaWsub(3-x)Msub(x)sup(V)vacantOsub(15-x/2)vacantsub(x/2) with hexagonal 5 L structure (sequence hhccc; space group P-3m1) could be prepared. The range of existence is restricted to Msup(V) containing compounds. With Msup(V) = Nb the lower phase boundary is x = 3. In the Ta system it is reached between x = 2 and 3; the pure Ta pervoskite (Ba:Ta:O = 6:3:13 1/2 = 4:2:9) represents the final member of the series Ba/sub 3/Srsub(1-y)Basub(y)Ta/sub 2/O/sub 9/ with y = 1.

  7. Benchmark calculations with simple phantom for neutron dosimetry (2)

    International Nuclear Information System (INIS)

    Yukio, Sakamoto; Shuichi, Tsuda; Tatsuhiko, Sato; Nobuaki, Yoshizawa; Hideo, Hirayama

    2004-01-01

    Benchmark calculations for high-energy neutron dosimetry were undertaken after SATIF-5. Energy deposition in a cylindrical phantom with 100 cm radius and 30 cm depth was calculated for the irradiation of neutrons from 100 MeV to 10 GeV. Using the ICRU four-element loft tissue phantom and four single-element (hydrogen, carbon, nitrogen and oxygen) phantoms, the depth distributions of deposition energy and those total at the central region of phantoms within l cm radius and at the whole region of phantoms within 100 cm radius were calculated. The calculated results of FLUKA, MCNPX, MARS, HETC-3STEP and NMTC/JAM codes were compared. It was found that FLUKA, MARS and NMTC/JAM showed almost the same results. For the high-energy neutron incident, the MCNP-X results showed the largest ones in the total deposition energy and the HETC-3STEP results show'ed smallest ones. (author)

  8. Double modulation of 5-fluorouracil by methotrexate and high-dose L-leucovorin in advanced colorectal cancer.

    Science.gov (United States)

    Romero, A O; Perez, J E; Cuevas, M A; Lacava, J A; Sabatini, C L; Dominguez, M E; Rodriguez, R; Barbieri, M R; Ortiz, E H; Salvadori, M A; Acuña, L A; Acuña, J M; Langhi, M J; Amato, S; Machiavelli, M R; Leone, B A; Vallejo, C T; Lorusso, V; DeLena, M

    1998-02-01

    A phase II trial was performed to evaluate the efficacy and toxicity of a double modulation of 5-fluorouracil (5-FU) by methotrexate (MTX) and L-leucovorin (L-LV) in patients with advanced recurrent (inoperable) or metastatic colorectal carcinoma (ACC). Between July 1993 and October 1995, 41 patients with ACC received a regimen that consisted of MTX 150 mg/m2 i.v., infused over a 20-minute period at hour 0, followed 19 hours later by L-LV 250 mg/m2 in a 2-hour i.v. infusion. 5-FU, 900 mg/m2, was administered by i.v. push injection at hour 20. Beginning 24 hours after MTX administration, all patients received four doses of L-LV, 15 mg/m2 i.m., every 6 hours. Cycles were repeated every 15 days. Two patients were not assessable for response. Objective regression was observed in 11 of 39 (28%) patients, [95% confidence interval (CI), 14-42%]. One (2%) patient achieved complete response (CR) and 10 (26%) partial response (PR). No change was recorded in 15 (39%) patients and progressive disease was noted in 13 (33%) patients. The median time to treatment failure was 6 months and the median survival time was 10 months. Toxicity was within acceptable limits, but one therapy-related death due to severe leukopenia was observed. The dose-limiting toxicity was mucositis. Eight episodes of grade 3 or 4 stomatitis were observed, and were responsible for dosage modifications of MTX and 5-FU. In conclusion, further in experimental and clinical studies are clearly necessary in order to design the best modulatory strategy of 5-FU.

  9. One-electron capture into Li-like autoionising N4+ (1s2ln'l') configurations by metastable N5+ (1s2s3S) multicharged ions in collisions with He and H2, observed by electron spectrometry at 3.4 keV amu-1

    International Nuclear Information System (INIS)

    Bordenave-Montesquieu, A.; Benoit-Cattin, P.; Gleizes, A.

    1985-01-01

    One-electron capture into N 4+ (1s2ln'l') configurations, with n'=2 to 4, has been observed by electron spectrometry when a N 5+ (1s2s 3 S) multicharged ion beam encounters an He or H 2 target, at low collision velocity (upsilon=0.37 au) within single-collision conditions. Contributions of other 1s2l metastable states and of the 1s 2 ground state may be disregarded. A small indication of two-electron capture by 1s2s 3 S ions into (1s2s 3 S)3l3l' configurations is also seen. (author)

  10. Cross-cultural measurement equivalence of the EQ-5D-5L items for English-speaking Asians in Singapore.

    Science.gov (United States)

    Luo, N; Wang, Y; How, C H; Wong, K Y; Shen, L; Tay, E G; Thumboo, J; Herdman, M

    2015-06-01

    To investigate how the response labels of the 5-level EQ-5D (EQ-5D-5L) items are interpreted and used by English-speaking Chinese and non-Chinese Singaporeans, as a means to assessing whether those items are cross-culturally equivalent health-status measures in this Asian population. In face-to-face interviews, Chinese, Malay and Indian visitors to a primary care institution in Singapore were asked to rate the relative severity conveyed by EQ-5D-5L response labels, each containing the keyword of 'no(t),' 'slight(ly),' 'moderate(ly),' 'severe(ly),' or 'unable'/'extreme(ly),' using a 0-100 numerical rating scale. Participants were also asked to describe 25 hypothetical health states using the EQ-5D-5L response labels. Differences between Chinese and Malay/Indian participants in label interpretation and selection were examined using multivariate regression analysis to adjust for participant characteristics. The differences in adjusted mean severity scores for individual EQ-5D-5L labels between Chinese (n = 148) and non-Chinese (Malay: n = 53; Indian: n = 56) participants ranged from 0.0 to 9.0. The relative severity of the labels to the participants supported the ordinality of the EQ-5D-5L response labels and was similar across ethnic groups. Chinese and non-Chinese participants selected similar response labels to describe each hypothetical health state, with the adjusted odds ratios of selecting any type of the five response labels for non-Chinese versus Chinese participants ranging from 0.92 to 1.15 (p > 0.05 for all). The EQ-5D-5L items are likely to generate equivalent health outcomes between English-speaking Chinese and non-Chinese Singaporeans.

  11. Hetero-metallic {3d-4f-5d} complexes: preparation and magnetic behavior of trinuclear [(L(Me2)Ni-Ln){W(CN)(8)}] compounds (Ln = Gd, Tb, Dy, Ho, Er, Y; L(Me2) = Schiff base) and variable SMM characteristics for the Tb derivative.

    Science.gov (United States)

    Sutter, Jean-Pascal; Dhers, Sébastien; Rajamani, Raghunathan; Ramasesha, S; Costes, Jean-Pierre; Duhayon, Carine; Vendier, Laure

    2009-07-06

    Assembling bimetallic {Ni-Ln}(3+) units and {W(CN)(8)}(3-) is shown to be an efficient route toward heteronuclear {3d-4f-5d} compounds. The reaction of either the binuclear [{L(Me2)Ni(H(2)O)(2)}{Ln(NO(3))(3)}] complexes or their mononuclear components [L(Me2)Ni] and Ln(NO(3))(3) with (HNBu(3))(3){W(CN)(8)} in dmf followed by diffusion of tetrahydrofuran yielded the trinuclear [{L(Me2)NiLn}{W(CN)(8)}] compounds 1 (Ln = Y), 2a,b (Gd), 3a,b (Tb), 4 (Dy), 5 (Ho), and 6 (Er) as crystalline materials. All of the derivatives possess the trinuclear core resulting from the linkage of the {W(CN)(8)} to the Ni center of the {Ni-Ln} unit. Differences are found in the solvent molecules acting as ligands and/or in the lattice depending on the crystallization conditions. For all the compounds ferromagnetic {Ni-W} and {Ni-Ln} (Ln = Gd, Tb, Dy, and Er} interactions are operative resulting in high spin ground states. Parameterization of the magnetic behaviors for the Y and Gd derivatives confirmed the strong cyano-mediated {Ni-W} interaction (J(NiW) = 27.1 and 28.5 cm(-1)) compared to the {Ni-Gd} interaction (J(NiGd) = 2.17 cm(-1)). The characteristic features for slow relaxation of the magnetization are observed for two Tb derivatives, but these are modulated by the crystal phase. Analysis of the frequency dependence of the alternating current susceptibility data yielded U(eff)/k(B) = 15.3 K and tau(0) = 4.5 x 10(-7) s for one derivative whereas no maxima of chi(M)'' appear above 2 K for the second one.

  12. A ribonuclease-resistant region of 5S RNA and its relation to the RNA binding sites of proteins L18 and L25

    DEFF Research Database (Denmark)

    Douthwaite, S; Garrett, R A; Wagner, R

    1979-01-01

    An RNA fragment, constituting three subfragments of nucleotide sequences 1-11, 69-87 and 89-120, is the most ribonuclease-resistant part of the native 5S RNA of Escherichia coli, at 0 degrees C. A smaller fragment of nucleotide sequence 69-87 and 90-110 is ribonuclease-resistant at 25 degrees....... Degradation of the L25-5S RNA complex with ribonuclease A or T2 yielded RNA fragments similar to those of the free 5S RNA at 0 degrees C and 25 degrees C; moreover L25 remained strongly bound to both RNA fragments and also produced some opening of the RNA structure in at least two positions. Protein L18...... initially protected most of the 5S RNA against ribonuclease digestion, at 0 degrees C, but was then gradually released prior to the formation of the larger RNA fragment. It cannot be concluded, therefore, as it was earlier (Gray et al., 1973), that this RNA fragment contains the primary binding site of L18....

  13. GLP-1 secretion is stimulated by 1,10-phenanthroline via colocalized T2R5 signal transduction in human enteroendocrine L cell

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jiyoung; Kim, Ki-Suk; Kim, Kang-Hoon; Lee, In-Seung; Jeong, Hyeon-soo; Kim, Yumi; Jang, Hyeung-Jin, E-mail: hjjang@khu.ac.kr

    2015-12-04

    Glucagon-like peptide-1 (GLP-1) hormone is known to regulate blood glucose by an insulinotropic effect and increases proliferation as and also prevents apoptosis of pancreatic β cells. We know that GLP-1 is secreted by nutrients such as fatty acids and sweet compounds but also bitter compounds via stimulation of G-protein coupled receptors (GPCRs) in the gut. Among these, bitter compounds are multiply-contained in phytochemicals or artificial materials and perceived as ligands of various bitter taste receptors. We hypothesized that GLP-1 hormone is secreted through stimulation of a single bitter taste receptor by 1,10-phenanthroline which is known agonist of taste receptor type 2 member 5 (T2R5). To prove this hypothesis, we used the representatively well-known 1,10-phenanthroline as ligand of single receptor and evaluated the existence of T2R5 by double-labeling immunofluorescence and then 1,10-phenanthroline is able to secrete GLP-1 hormone through stimulation of T2R5 in human enteroendocrine cells. Consequently, we verify that GLP-1 hormone is colocalized with T2R5 in the human duodenum and ileum tissue and is secreted by 1,10-phenanthroline via T2R5 signal transduction in differentiated human enteroendocrine L cells. - Highlights: • Taste receptor type 2 member 5 (T2R5) is colocalized with GLP-1 hormone in human enteroendocrine cells. • GLP-1 secretion is stimulated by 1,10-phenanthroline via stimulation of T2R5. • Inhibition of the bitter taste pathway reduce GLP-1 secretion.

  14. GLP-1 secretion is stimulated by 1,10-phenanthroline via colocalized T2R5 signal transduction in human enteroendocrine L cell

    International Nuclear Information System (INIS)

    Park, Jiyoung; Kim, Ki-Suk; Kim, Kang-Hoon; Lee, In-Seung; Jeong, Hyeon-soo; Kim, Yumi; Jang, Hyeung-Jin

    2015-01-01

    Glucagon-like peptide-1 (GLP-1) hormone is known to regulate blood glucose by an insulinotropic effect and increases proliferation as and also prevents apoptosis of pancreatic β cells. We know that GLP-1 is secreted by nutrients such as fatty acids and sweet compounds but also bitter compounds via stimulation of G-protein coupled receptors (GPCRs) in the gut. Among these, bitter compounds are multiply-contained in phytochemicals or artificial materials and perceived as ligands of various bitter taste receptors. We hypothesized that GLP-1 hormone is secreted through stimulation of a single bitter taste receptor by 1,10-phenanthroline which is known agonist of taste receptor type 2 member 5 (T2R5). To prove this hypothesis, we used the representatively well-known 1,10-phenanthroline as ligand of single receptor and evaluated the existence of T2R5 by double-labeling immunofluorescence and then 1,10-phenanthroline is able to secrete GLP-1 hormone through stimulation of T2R5 in human enteroendocrine cells. Consequently, we verify that GLP-1 hormone is colocalized with T2R5 in the human duodenum and ileum tissue and is secreted by 1,10-phenanthroline via T2R5 signal transduction in differentiated human enteroendocrine L cells. - Highlights: • Taste receptor type 2 member 5 (T2R5) is colocalized with GLP-1 hormone in human enteroendocrine cells. • GLP-1 secretion is stimulated by 1,10-phenanthroline via stimulation of T2R5. • Inhibition of the bitter taste pathway reduce GLP-1 secretion.

  15. O(5)sub(L)xO(5)sub(R)xU(1)sub(V) electro-weak gauge theory and the neutrino pairing mechanism

    International Nuclear Information System (INIS)

    Samiullah, M.; Mubarak, A.

    1981-08-01

    The possibility of using the group O(5)sub(L)xO(5)sub(R)xU(1)sub(V) for unifying the weak and electromagnetic interactions is studied. We are led to an anomaly free theory. Potentially the theory has the advantage of incorporating the previous results. For example, all the results of O(5)sub(L)xU(1) studies are, as a special case, obtainable at low energies. In the process of breaking the symmetry down to Weinberg-Salam theory at the level of SU(2)sub(L)xSU(2)sub(R)xU(1)sub(V), we have employed the neutrino proposed by Mannheim. We have been able to reproduce several of the conventional electroweak aspects such as the parity violation in both the lepton and charged quark sectors, Weinberg mixing pattern in the neutral current sector while keeping the left-handed neutrinos massless. All the salient features of low energy phenomenology are shown to follow. (author)

  16. 10 K gate I(2)L and 1 K component analog compatible bipolar VLSI technology - HIT-2

    Science.gov (United States)

    Washio, K.; Watanabe, T.; Okabe, T.; Horie, N.

    1985-02-01

    An advanced analog/digital bipolar VLSI technology that combines on the same chip 2-ns 10 K I(2)L gates with 1 K analog devices is proposed. The new technology, called high-density integration technology-2, is based on a new structure concept that consists of three major techniques: shallow grooved-isolation, I(2)L active layer etching, and I(2)L current gain increase. I(2)L circuits with 80-MHz maximum toggle frequency have developed compatibly with n-p-n transistors having a BV(CE0) of more than 10 V and an f(T) of 5 GHz, and lateral p-n-p transistors having an f(T) of 150 MHz.

  17. Application of the methodology of surface of answer in the determination of the PCT in the simulation of a LOFT; Aplicacion de la metodologia de superficies de respuesta en la determinacion del PCT en la simulacion de un LOFT

    Energy Technology Data Exchange (ETDEWEB)

    Alva N, J. [IPN, Escuela Superior de Fisica y Matematicas, Departamento de Ingenieria Nuclear, Av. IPN s/n, Col. Lindavista, Mexico 07738 D.F. (Mexico); Ortiz V, J.; Amador G, R. [ININ, 52750 La Marquesa, Estado de Mexico (Mexico)]. e-mail: neriaesfm@gmail.com

    2008-07-01

    This article summarizes the main typical of the methodology of surfaces and answer (MSA) and its connections with the lineal regression analysis. Also, an example of the application of MSA in the prediction of the principle cladding temperature (PCT) of a combustible assembly of a nuclear reactor, whose used data were taken from the simulation of a LOFT (Loss Of Fluid Test) during a course of experts. The made prediction will be used like one first approach to predict the behavior of the PCT, this is made in order to diminish the time of calculation when realizing the executions of codes thermal hydraulics of better estimation. The present work comprises of the theoretical base of the project in charge to delineate a methodology of uncertainty analysis for codes of better estimation, employees in the thermal hydraulics analysis and safety of plants and nuclear reactors. The institutions that participate in such project are: ININ, CFE, IPN and CNSNS, is possible to mention that this project is sponsored by the IAEA. (Author)

  18. Silsesquioxane organofunctionalized with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole: Preparation and subsequent reaction with silver and potassium hexacyanoferrate(III) for detection of L-cysteine

    International Nuclear Information System (INIS)

    Carmo, Devaney R. do; Silvestrini, Daniela R.; Silveira, Tayla F.S. da; Cumba, Loanda R.; Dias Filho, Newton L.; Soares, Layciane A.

    2015-01-01

    The octakis(3-chloropropyl)octasilsesquioxane (SS) was organofunctionalized with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (Purpald). The functionalized silsesquioxane with Purpald (SP) was characterized by Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR). After functionalized, silsesquioxane can interact with silver nitrate and subsequently with potassium hexacyanoferrate (III) (AgHSP). The novel hybrid composite formed (AgHSP) was characterized by Fourier transform infrared spectra, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX). AgHSP was electrochemically characterized by cyclic voltammetry (CV) using graphite paste electrode. The AgHSP incorporated into a graphite paste electrode (20% w/w) was tested for detection of L-cysteine. The modified electrode showed a linear response from 9.0 × 10 −5 to 5.0 × 10 −3 mol L −1 with the corresponding equation Y(A) = 0.01315 + 1.865 [L-cysteine], and a correlation coefficient of r 2 = 0.9995. The method showed a detection limit of 1.76 × 10 −4 mol L −1 with a relative standard deviation of ± 2% (n = 3) and amperometric sensitivity of 1.865 A/mol L −1 . - Highlights: • Functionalization of silsesquioxane with Purpald compound • Characterization of silsesquioxane was done with FT-IR, NMR, SEM, EDX and VC. • The modified silsesquioxane was interacted with AgNO 3 and K 3 [Fe(CN) 6 ]. • The composite was tested by cyclic voltammetry for detection of L-cysteine

  19. Silsesquioxane organofunctionalized with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole: Preparation and subsequent reaction with silver and potassium hexacyanoferrate(III) for detection of L-cysteine

    Energy Technology Data Exchange (ETDEWEB)

    Carmo, Devaney R. do, E-mail: docarmo@dfq.feis.unesp.br; Silvestrini, Daniela R.; Silveira, Tayla F.S. da; Cumba, Loanda R.; Dias Filho, Newton L.; Soares, Layciane A.

    2015-12-01

    The octakis(3-chloropropyl)octasilsesquioxane (SS) was organofunctionalized with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (Purpald). The functionalized silsesquioxane with Purpald (SP) was characterized by Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR). After functionalized, silsesquioxane can interact with silver nitrate and subsequently with potassium hexacyanoferrate (III) (AgHSP). The novel hybrid composite formed (AgHSP) was characterized by Fourier transform infrared spectra, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX). AgHSP was electrochemically characterized by cyclic voltammetry (CV) using graphite paste electrode. The AgHSP incorporated into a graphite paste electrode (20% w/w) was tested for detection of L-cysteine. The modified electrode showed a linear response from 9.0 × 10{sup −5} to 5.0 × 10{sup −3} mol L{sup −1} with the corresponding equation Y(A) = 0.01315 + 1.865 [L-cysteine], and a correlation coefficient of r{sup 2} = 0.9995. The method showed a detection limit of 1.76 × 10{sup −4} mol L{sup −1} with a relative standard deviation of ± 2% (n = 3) and amperometric sensitivity of 1.865 A/mol L{sup −1}. - Highlights: • Functionalization of silsesquioxane with Purpald compound • Characterization of silsesquioxane was done with FT-IR, NMR, SEM, EDX and VC. • The modified silsesquioxane was interacted with AgNO{sub 3} and K{sub 3}[Fe(CN){sub 6}]. • The composite was tested by cyclic voltammetry for detection of L-cysteine.

  20. Enhanced photocurrent in RuL2(NCS)2/di-(3-aminopropyl)-viologen/SnO2/ITO system

    International Nuclear Information System (INIS)

    Lee, Wonjoo; Kwak, Chang Gon; Mane, R.S.; Min, Sun Ki; Cai, Gangri; Ganesh, T.; Koo, Gumae; Chang, Jinho; Cho, Byung Won; Kim, Sei-Ki; Han, Sung-Hwan

    2008-01-01

    A Ru(2,2'-bipyridine-4,4'-dicarboxylic acid) 2 (NCS) 2 [RuL 2 (NCS) 2 ]/di-(3-aminopropyl)-viologen (DAPV)/tin oxide (SnO 2 ) system was prepared and applied to extensive photocurrent generation with its maximum surface area. The SnO 2 thin films on tin-doped indium oxide (ITO) were prepared using the chemical bath deposition method. Then, RuL 2 (NCS) 2 /DAPV on SnO 2 /ITO was easily prepared using self-assembled monolayers (SAMs). The photocurrent measurement of the system showed an excellent photocurrent of 20 nA cm -2 under the air mass 1.5 conditions (100 mW cm -2 ), which was increased by a factor of four compared to ones without SnO 2 layers

  1. Distraction manipulation reduction of an L5-S1 disk herniation.

    Science.gov (United States)

    Cox, J M; Hazen, L J; Mungovan, M

    1993-06-01

    A computed tomography (CT)-confirmed L5-S1 disk protrusion is reported to be reduced following chiropractic adjustment, as seen on repeat CT scanning. Correlation of the CT reports with the patient's symptoms before and after manipulation is reported. A 38-yr-old female was treated for low back pain and right lower extremity first sacral dermatome sciatica. CT confirmed disk herniations at both the L4-L5 and L5-S1 levels were found. Motor weakness of the right gluteus maximus muscle was found and extremely tight hamstring muscles accompanying positive straight leg signs were elicited. A clinical and imaging diagnosis of an L5-S1 disk herniation was made. Distraction type chiropractic manipulation, electrical stimulation, exercises, nutrition advice and low back wellness class were administered with complete relief of sciatic pain and nearly complete relief of low back pain. Chiropractic distraction manipulation is an effective treatment of lumbar disk herniation, if the chiropractor is observant during its administration for patient tolerance to manipulation under distraction and any signs of neurological deficit demanding other types of care.

  2. Evidence from adult L1 Afrikaans L2 French

    African Journals Online (AJOL)

    results of this study show that a large number of the L2 learners had indeed acquired ... position in V2-languages (such as German) and in third position in non-V2 ... L1, allows construction types x and y but he will have no problem acquiring .... Modern Foreign Languages at Stellenbosch University at the time of testing.

  3. Folic acid and L-5-methyltetrahydrofolate: comparison of clinical pharmacokinetics and pharmacodynamics.

    Science.gov (United States)

    Pietrzik, Klaus; Bailey, Lynn; Shane, Barry

    2010-08-01

    There is a large body of evidence to suggest that improving periconceptional folate status reduces the risk of neonatal neural tube defects. Thus increased folate intake is now recommended before and during the early stages of pregnancy, through folic acid supplements or fortified foods. Furthermore, there is growing evidence that folic acid may have a role in the prevention of other diseases, including dementia and certain types of cancer. Folic acid is a synthetic form of the vitamin, which is only found in fortified foods, supplements and pharmaceuticals. It lacks coenzyme activity and must be reduced to the metabolically active tetrahydrofolate form within the cell. L-5-methyl-tetrahydrofolate (L-5-methyl-THF) is the predominant form of dietary folate and the only species normally found in the circulation, and hence it is the folate that is normally transported into peripheral tissues to be used for cellular metabolism. L-5-methyl-THF is also available commercially as a crystalline form of the calcium salt (Metafolin(R)), which has the stability required for use as a supplement. Studies comparing L-5-methyl-THF and folic acid have found that the two compounds have comparable physiological activity, bioavailability and absorption at equimolar doses. Bioavailability studies have provided strong evidence that L-5-methyl-THF is at least as effective as folic acid in improving folate status, as measured by blood concentrations of folate and by functional indicators of folate status, such as plasma homocysteine. Intake of L-5-methyl-THF may have advantages over intake of folic acid. First, the potential for masking the haematological symptoms of vitamin B(12) deficiency may be reduced with L-5-methyl-THF. Second, L-5-methyl-THF may be associated with a reduced interaction with drugs that inhibit dihydrofolate reductase.

  4. Simulation of the ACE L2 and ACE L5 MCCI experiment under dry surface conditions with ASTEC MEDICIS using an effective heat transfer model

    Energy Technology Data Exchange (ETDEWEB)

    Agethen, Kathrin; Koch, Marco K. [Bochum Univ. (Germany). Reactor Simulation and Safety Group

    2013-07-01

    In a postulated severe accident the loss of cooling can lead to a melting of the core and to a failure of the vessel. The molten core material discharges to the containment cavity and interacts with the concrete basemat. The heat up of the concrete leads to the release of sparing gases (H{sub 2}, CO{sub 2}, SiO), which stir the pool und causes chemical reactions. Especially the metals (Zr, Fe, Ni, Cr) in the corium are oxidized und the exothermic energy is released to the melt, which raises the melt temperature further. The release of combustible gases (H{sub 2}, CO) and fission products to the containment atmosphere occurs as a result. In the long time (>10 h) containment failure and basemat penetration may occur, which can lead to fission product release to the environment. For further development and validation, simulations of experiments in which molten core concrete interaction (MCCI) is investigated, are necessary. In this work the new available effective heat transfer model in MEDICIS is used to calculate experiments of the ACE program, in which generic corium material is heated up and interacts with the concrete basemat. Here, especially the ACE L2 experiment with siliceous concrete and the ACE L5 experiment with limestone common sand (LCS) concrete will be presented. These experiments enable to analyze the heat transfer from the interior of the melt to the upper surface under dry conditions. Secondary the modeling in ASTEC version 2.p2 with the effective heat transfer module in MEDICIS is described. Results of MEDICIS simulations will be discussed by means of phenomena like ablation behavior and erosions depth, layer temperature and surface heat loss. Finally the issue of an effective heat transfer coefficient for the surface under dry conditions without top flooding is figured out. (orig.)

  5. Exonuclease hDIS3L2 specifies an exosome-independent 3'-5' degradation pathway of human cytoplasmic mRNA

    DEFF Research Database (Denmark)

    Lubas, Michal Szymon; Damgaard, Christian Kroun; Tomecki, Rafal

    2013-01-01

    Turnover of mRNA in the cytoplasm of human cells is thought to be redundantly conducted by the monomeric 5'-3' exoribonuclease hXRN1 and the 3'-5' exoribonucleolytic RNA exosome complex. However, in addition to the exosome-associated 3'-5' exonucleases hDIS3 and hDIS3L, the human genome encodes...

  6. Enzymatic extraction of pectin from artichoke (Cynara scolymus L.) by-products using Celluclast®1.5L.

    Science.gov (United States)

    Sabater, Carlos; Corzo, Nieves; Olano, Agustín; Montilla, Antonia

    2018-06-15

    The aim of this study was to optimise pectin extraction from artichoke by-products with Celluclast ® 1.5L using an experimental design analysed by response-surface methodology (RSM). The variables optimised were artichoke by-product powder concentration (2-7%, X 1 ), enzyme dose (2.2-13.3 U g -1 , X 2 ) and extraction time (6-24 h, X 3 ). The variables studied were galacturonic acid (GalA) (R 2 93.9) and pectic neutral sugars (R 2 92.8) content and pectin yield (R 2 88.6). In the optimum extraction conditions (X 1  = 6.5%; X 2  = 10.1 U g -1 ; X 3  = 27.2 h), pectin yield was 176 mgg -1 dry matter (DM). Considering 27.2 h of treatment as the +α value given by the design, the extraction time was increased up to 48 h obtaining a yield of 221 mg g -1 DM. The enzymatic method optimised allows obtaining artichoke pectin with good yield, high GalA (720 mg g -1 DM) and arabinose (127.6mgg -1 DM) contents and degree of methylation of 19.5%. Copyright © 2018 Elsevier Ltd. All rights reserved.

  7. Reversal of methylation silencing of Apo2L/TRAIL receptor 1 (DR4) expression overcomes resistance of SK-MEL-3 and SK-MEL-28 melanoma cells to interferons (IFNs) or Apo2L/TRAIL.

    Science.gov (United States)

    Bae, S I; Cheriyath, V; Jacobs, B S; Reu, F J; Borden, E C

    2008-01-17

    Human melanoma cell lines, SK-MEL-3 and SK-MEL-28, despite induction of the proapoptotic cytokine, Apo2L/TRAIL, did not undergo apoptosis in response to interferons (IFN-alpha2b or IFN-beta). Postulating that genes important for apoptosis induction by IFNs might be silenced by methylation, the DNA demethylating agent 5-aza-2'-deoxycytidine (5-AZAdC) was assessed. DR4 (TRAIL-R1) was identified as one of the genes reactivated by 5-AZAdC with a >3-fold increase in 8 of 10 melanoma cell lines. Pretreatment with 5-AZAdC sensitized SK-MEL-3 and SK-MEL-28 cells to apoptosis induced by IFN-alpha2b and IFN-beta; methylation-specific PCR and bisulfite sequencing confirmed demethylation of 5'CpG islands of DR4 and flow cytometry showed an increase in DR4 protein on the cell surface. In cells with reactivated DR4, neutralizing mAB to TRAIL reduced apoptosis in response to IFN-beta or Apo2L/TRAIL. To further confirm the role of DR4, it was expressed by retroviral vector in SK-MEL-3 and SK-MEL-28 cells with reversal of resistance to IFN-beta and Apo2L/TRAIL. Thus, reexpressing DR4 by 5-AZAdC or retroviral transfection in melanoma cell in which promoter methylation had suppressed its expression, potentiated apoptosis by IFN-alpha2b, IFN-beta and Apo2L/TRAIL. Reactivation of silenced proapoptotic genes by inhibitors of DNA methylation may enhance clinical response to IFNs or Apo2L/TRAIL.

  8. A Head-to-Head Comparison of UK SF-6D and Thai and UK EQ-5D-5L Value Sets in Thai Patients with Chronic Diseases.

    Science.gov (United States)

    Sakthong, Phantipa; Munpan, Wipaporn

    2017-10-01

    Little was known about the head-to-head comparison of psychometric properties between SF-6D and EQ-5D-5L or the different value sets of EQ-5D-5L. Therefore, this study set out to compare the psychometric properties including agreement, convergent, and known-group validity between the SF-6D and the EQ-5D-5L using the real value sets from Thailand and the UK in patients with chronic diseases. 356 adults taking a medication for at least 3 months were identified from a university hospital in Bangkok, Thailand, between July 2014 and March 2015. Agreement was assessed by intraclass correlation coefficients (ICCs) and Bland-Altman plots. Convergent validity was evaluated using Spearman's rank correlation coefficients between SF-6D and EQ-5D-5L and EQ-VAS and SF-12v2. For known-groups validity, the Mann-Whitney U test and Kruskal-Wallis test were used to examine the associations between SF-6D and EQ-5D-5L and patient characteristics. Agreements between the SF-6D and the EQ-5D-5L using Thai and UK value sets were fair, with ICCs of 0.45 and 0.49, respectively. Bland-Altman plots showed that the majority of the SF-6D index scores were lower than the EQ-5D-5L index scores. Both the EQ-5D-5L value sets were more related to the EQ-VAS and physical health, while the SF-6D was more associated with mental health. Both EQ-5D-5L value sets were more sensitive than the SF-6D in discriminating patients with different levels of more known groups except for adverse drug reactions. The SF-6D and both EQ-5D-5L value sets appeared to be valid but sensitive to different outcomes in Thai patients with chronic diseases.

  9. The L2 Motivational Self System and L2 Achievement: A Study of Saudi EFL Learners

    Science.gov (United States)

    Moskovsky, Christo; Assulaimani, Turki; Racheva, Silvia; Harkins, Jean

    2016-01-01

    The research reported in this article explores the relationship between Dörnyei's (2005, 2009) Second Language Motivational Self System (L2MSS) and the L2 proficiency level of Saudi learners of English as a foreign language (EFL). Male and female participants (N = 360) responded to a questionnaire relating to the main components of L2MSS, the…

  10. L4-L5 compression and anterior/posterior joint shear forces in cabin attendants during the initial push/pull actions of airplane meal carts.

    Science.gov (United States)

    Sandfeld, Jesper; Rosgaard, Christian; Jensen, Bente Rona

    2014-07-01

    The aim of the present study was to assess the acute low back load of cabin attendants during cart handling and to identify working situations which present the highest strain on the worker. In a setup, 17 cabin attendants (ten females and seven males) pushed, pulled and turned a 20 kg standard meal cart (L: 0.5m × W: 0.3 m × H: 0.92 m) loaded with extra 20 kg and 40 kg, respectively on two different surfaces (carpet and linoleum) and at three floor inclinations (-2°, 0° and +2°). Two force transducers were mounted as handles. Two-dimensional movement analysis was performed and a 4D WATBAK modelling tool was used to calculate the acute L4-L5 load. No working situations created loads greater than the accepted values for single exertions, however compression and anterior/posterior shear forces during pulling and turning were much higher when compared with pushing. There were significant effects of handling the cart on different floor types, at the varying inclinations and with different cart weights. Additionally, when external forces were reduced, the cabin attendants did not decrease push/pull force proportionally and thus the L4-L5 load did not decrease as much as expected. Copyright © 2014 Elsevier Ltd and The Ergonomics Society. All rights reserved.

  11. L1000CDS2: LINCS L1000 characteristic direction signatures search engine.

    Science.gov (United States)

    Duan, Qiaonan; Reid, St Patrick; Clark, Neil R; Wang, Zichen; Fernandez, Nicolas F; Rouillard, Andrew D; Readhead, Ben; Tritsch, Sarah R; Hodos, Rachel; Hafner, Marc; Niepel, Mario; Sorger, Peter K; Dudley, Joel T; Bavari, Sina; Panchal, Rekha G; Ma'ayan, Avi

    2016-01-01

    The library of integrated network-based cellular signatures (LINCS) L1000 data set currently comprises of over a million gene expression profiles of chemically perturbed human cell lines. Through unique several intrinsic and extrinsic benchmarking schemes, we demonstrate that processing the L1000 data with the characteristic direction (CD) method significantly improves signal to noise compared with the MODZ method currently used to compute L1000 signatures. The CD processed L1000 signatures are served through a state-of-the-art web-based search engine application called L1000CDS 2 . The L1000CDS 2 search engine provides prioritization of thousands of small-molecule signatures, and their pairwise combinations, predicted to either mimic or reverse an input gene expression signature using two methods. The L1000CDS 2 search engine also predicts drug targets for all the small molecules profiled by the L1000 assay that we processed. Targets are predicted by computing the cosine similarity between the L1000 small-molecule signatures and a large collection of signatures extracted from the gene expression omnibus (GEO) for single-gene perturbations in mammalian cells. We applied L1000CDS 2 to prioritize small molecules that are predicted to reverse expression in 670 disease signatures also extracted from GEO, and prioritized small molecules that can mimic expression of 22 endogenous ligand signatures profiled by the L1000 assay. As a case study, to further demonstrate the utility of L1000CDS 2 , we collected expression signatures from human cells infected with Ebola virus at 30, 60 and 120 min. Querying these signatures with L1000CDS 2 we identified kenpaullone, a GSK3B/CDK2 inhibitor that we show, in subsequent experiments, has a dose-dependent efficacy in inhibiting Ebola infection in vitro without causing cellular toxicity in human cell lines. In summary, the L1000CDS 2 tool can be applied in many biological and biomedical settings, while improving the extraction of

  12. Plasma-Column Equilibrium in Tokamak-5; L'Equilibre d'une Colonne de Plasma dans l'Installation Tokamak-5; Issledovanie ravnovesiya plazmennogo shnura na ustanovke Tokamak-5; El Equilibrio de una Columna de Plasma en la Instalacion 'Tokamak-5'

    Energy Technology Data Exchange (ETDEWEB)

    Muhovatov, V. S. [Institut Atomnoj Ehnergii, Im. I.V. Kurchatova, Moskva, SSSR (Russian Federation)

    1966-04-15

    'observe egalement lorsque les segments sont isoles. L'auteur examine les causes possibles de cette divergence et celles qui provoquent l'apparition d'une forte tension entre les segments. (author) [Spanish] Esta memoria se refiere al estudio de las condiciones de equilibrio de una columna de plasma en la instalacion Tokamak-5. Los parametros de la instalacion son los siguientes: radio mayor de la camara de cobre R = 62,5 cm, radio menor b = 25 cm, radio menor del revestimiento = 20 cm, radio de la abertura del diafragma = 15 cm (el centro del diafragma coincide con el centro de la seccion transversal de la vasija); intensidad del campo magnetico axial B{sub 0} = 6 a 12 kOe, intensidad de la corriente en el plasma I = 15 a 20 kA. Se han efectuado los experimentos con una presion inicial del hidrogeno (1,8 a 5,0) * 10{sup -4} Torr. Entre la camara y el revestimiento se colocaron unos conductores mediante los cuales so originaba, en el volumen de la camara, un campo magnetico transversal B{sub z}, que variaba sincronicamente con la corriente de descarga. En una memoria anterior se demostro la concordancia cualitativa de los resultados experimentales con la hipotesis de que la columna de plasma es mantenida en equilibrio por la camara de cobre. Se indicaba tambien que la conductividad media del plasma respecto de la seccion de la columna alcanza su valor maximo si, por medio de un campo transversal B{sub z}, se situa la columna concentricamente respecto de la abertura del diafragma. En la presente memoria se muestra que, en la mayoria de los regimenes de descarga se tiene buena concordancia cualitativa entre los datos experimentales y la teorfa del equilibrio de la columna del plasma dentro de una camara conductora. Sin embargo, en una serie de regimenes de descarga (con bajas presiones iniciales del gas y valores elevados del desplazamiente {Delta}{sub B} = b{sup 2}B{sub z}c/2J hacia la pared interior del toro) se observo cierta discrepancia entre los datos teoricos y experimentales

  13. Radiosynthesis and biological evaluation of 5-(3-[18F]Fluoropropyloxy)-L-tryptophan for tumor PET imaging

    International Nuclear Information System (INIS)

    He, Shanzhen; Tang, Ganghua; Hu, Kongzhen; Wang, Hongliang; Wang, Shuxia; Huang, Tingting; Liang, Xiang; Tang, Xiaolan

    2013-01-01

    Introduction: [ 18 F]FDG PET has difficulty distinguishing tumor from inflammation in the clinic because of the same high uptake in nonmalignant and inflammatory tissue. In contrast, amino acid tracers do not accumulate in inflamed tissues and thus provide an excellent opportunity for their use in clinical cancer imaging. In this study, we developed a new amino acid tracer 5-(3-[ 18 F]Fluoropropyloxy)-L-tryptophan ([ 18 F]-L-FPTP) by two-step reactions and performed its biologic evaluation. Methods: [ 18 F]-L-FPTP was prepared by [ 18 F]fluoropropylation of 5-hydroxy-L-tryptophan disodium salt and purification on C18 cartridges. The biodistribution of [ 18 F]-L-FPTP was determined in normal mice and the incorporation of [ 18 F]-L-FPTP into tissue proteins was investigated. In vitro competitive inhibition experiments were performed with Hepa1-6 hepatoma cell lines. [ 18 F]-L-FPTP PET imaging was performed on tumor-bearing and inflammation mice and compared with [ 18 F]-L-FEHTP PET. Results: The overall uncorrected radiochemical yield of [ 18 F]-L-FPTP was 21.1 ± 4.4% with a synthesis time of 60 min, the radiochemical purity was more than 99%. Biodistribution studies demonstrate high uptake of [ 18 F]-L-FPTP in liver, kidney, pancreas, and blood at the early phase, and fast clearance in most tissues over the whole observed time. The uptake studies in Hepa1-6 cells suggest that [ 18 F]-L-FPTP is transported by the amino acid transport system B 0,+ , LAT2 and ASC. [ 18 F]-L-FPTP displays good stability and is not incorporated into proteins in vitro. PET imaging shows that [ 18 F]-L-FPTP can be a better potential PET tracer for differentiating tumor from inflammation than [ 18 F]FDG and 5-(3-[ 18 F]fluoroethyloxy)-L-tryptophan ([ 18 F]-L-FEHTP), with high [ 18 F]-L-FPTP uptake ratio (2.53) of tumor to inflammation at 60 min postinjection. Conclusions: Using [ 18 F]fluoropropyl derivatives as intermediates, the new tracer [ 18 F]-L-FPTP was achieved with good yield and

  14. Exact solutions of sl-boson system in U(2l + 1) reversible O(2l + 2) transitional region

    CERN Document Server

    Zhang Xin

    2002-01-01

    Exact eigen-energies and the corresponding wavefunctions of the interacting sl-boson system in U(2l + 1) reversible O(2l +2) transitional region are obtained by using an algebraic Bethe Ansatz with the infinite dimensional Lie algebraic technique. Numerical algorithm for solving the Bethe Ansatz equations by using mathematical package is also outlined

  15. Gaming as Extramural English L2 Learning and L2 Proficiency among Young Learners

    Science.gov (United States)

    Sylven, Liss Kerstin; Sundqvist, Pia

    2012-01-01

    Today, playing digital games is an important part of many young people's everyday lives. Claims have been made that certain games, in particular massively multiplayer online role-playing games (MMORPGs) provide L2 English learners with a linguistically rich and cognitively challenging virtual environment that may be conducive to L2 learning, as…

  16. Fra web 2.0 til læringsspil 2.0

    DEFF Research Database (Denmark)

    Duus Henriksen, Thomas

    2010-01-01

    Artiklen sætter fokus på, hvordan man kan omsætte web 2.0-teknologien til læringsspil for derigennem at skabe en brugerbaseret, deltagerdreven tilgang til at arbejde med læringsspil. Artiklen beretter om, hvordan organisationsudviklingsspillet 6Styles omsættes, først til en skoleudviklingssammenh......Artiklen sætter fokus på, hvordan man kan omsætte web 2.0-teknologien til læringsspil for derigennem at skabe en brugerbaseret, deltagerdreven tilgang til at arbejde med læringsspil. Artiklen beretter om, hvordan organisationsudviklingsspillet 6Styles omsættes, først til en...... skoleudviklingssammenhæng og dernæst til et såkaldt 2.0-læringsspil, hvor deltagerne skaber læringsspillets indhold, mens de spiller spillet. Erfaringer fra brug af læringsspillet peger på, at 1) 2.0 læringsspil tager markant mere tid at anvende, 2) deltagerne udvikler en produktiv forståelse for den underliggende teori...

  17. New palladium(II) and platinum(II) 5,5-diethylbarbiturate complexes with 2-phenylpyridine, 2,2'-bipyridine and 2,2'-dipyridylamine: synthesis, structures, DNA binding, molecular docking, cellular uptake, antioxidant activity and cytotoxicity.

    Science.gov (United States)

    Icsel, Ceyda; Yilmaz, Veysel T; Kaya, Yunus; Samli, Hale; Harrison, William T A; Buyukgungor, Orhan

    2015-04-21

    Novel palladium(ii) and platinum(ii) complexes of 5,5-diethylbarbiturate (barb) with 2-phenylpyridine (Hppy), 2,2'-bipyridine (bpy) and 2,2'-dipyridylamine (dpya) have been prepared and characterized by elemental analysis, IR, UV-Vis, NMR and ESI-MS. Single-crystal diffraction measurements show that complex consists of binuclear [Pd2(μ-barb-κN,O)2(ppy-κN,C)2] moieties, while complexes are mononuclear, [M(barb-κN)2(L-κN,N')] (L = bpy or dpya). has a composition of [Pt(dpya-κN,N')2][Ag(barb-κN)2]2·4H2O and was assumed to have a structure of [Pt(barb-κN)(Hppy-κN)(ppy-κN,C)]·3H2O. The complexes were found to exhibit significant DNA binding affinity by a non-covalent binding mode, in accordance with molecular docking studies. In addition, complexes and displayed strong binding with supercoiled pUC19 plasmid DNA. Cellular uptake studies were performed to assess the subcellular localization of the selected complexes. A moderate radical scavenging activity of and was confirmed by DPPH and ABTS tests. Complexes , , and showed selectivity against HT-29 (colon) cell line.

  18. Pharmacokinetics in Wistar Rats of 5-[(4-Carboxybutanoyl)Amino]-2-Hydroxybenzoic Acid: A Novel Synthetic Derivative of 5-Aminosalicylic Acid (5-ASA) with Possible Anti-Inflammatory Activity

    Science.gov (United States)

    Correa-Basurto, José; Rosales Hernández, Martha Cecilia; Padilla Martínez, Itzia Irene; Mendieta-Wejebe, Jessica Elena

    2016-01-01

    5-[(4-carboxybutanoyl)amino]-2-hydroxybenzoic acid (C2) is a novel synthetic derivative of 5-aminosalicylic acid (5-ASA), which is currently being evaluated ex vivo as an anti-inflammatory agent and has shown satisfactory results. This study aimed to obtain the pharmacokinetic profiles, tissue distribution and plasma protein binding of C2 in Wistar Rats. Additionally, an HPLC method was developed and validated to quantify C2 in rat plasma. The pharmacokinetic profiles of intragastric, intravenous and intraperitoneal administration routes at singles doses of 100, 50, and 100 mg/kg, respectively, were studied in Wistar rats. The elimination half-life of intravenously administered C2 was approximately 33 min. The maximum plasma level of C2 was reached approximately 24 min after intragastric administration, with a Cmax value of 2.5 g/mL and an AUCtot value of 157 μg min-1/mL; the oral bioavailability was approximately 13%. Following a single intragastric or oral dose (100 mg/kg), C2 was distributed and detected in all examined tissues (including the brain and colon). The results showed that C2 accumulates over time. The plasma protein binding results indicated that the unbound fraction of C2 at concentrations of 1 to 20 μg/mL ranged from 89.8% to 92.5%, meaning that this fraction of C2 is available to cross tissues. Finally, the blood-plasma partitioning (BP ratio) of C2 in rat plasma was 0.71 and 0.6 at concentrations of 5 and 10 μg/mL, respectively, which indicates that C2 is free in the plasmatic phase and not inside blood cells. The results of this study suggest that a fraction of the administered C2 dose is absorbed in the stomach, and the fraction that is not absorbed reaches the small intestine and colon. This distribution constitutes the main advantage of C2 compared with 5-ASA for the treatment of ulcerative colitis (UC) and Crohn's disease (CD). PMID:27454774

  19. Characterization and immunogenicity of rLipL32/1-LipL21-OmpL1/2 fusion protein as a novel immunogen for a vaccine against Leptospirosis

    Directory of Open Access Journals (Sweden)

    Zhao Xin

    2015-01-01

    Full Text Available Vaccination is an effective strategy to prevent leptospirosis, a global zoonotic disease caused by infection with pathogenic Leptospira species. However, the currently used multiple-valence vaccine, which is prepared with whole cells of several Leptospira serovars, has major side effects, while its cross-immunogenicity among different Leptospira serovars is weak. LipL32, LipL21 and 2 OmpL1 have been confirmed as surface-exposed antigens in all pathogenic Leptospira strains, but their immunoprotective efficiency needs to be improved. In the present study, we generated a fusion gene lipL32/1-lipL21-ompL1/2 using primer-linking PCR and an engineered E. coli strain to express the recombinant fusion protein rLipL32/1-LipL21-OmpL1/2 (rLLO. Subsequently, the expression conditions were optimized using a central composite design that increased the fusion protein yield 2.7-fold. Western blot assays confirmed that rLLO was recognized by anti-rLipL32/1, anti-rLipL21, and anti-rOmpL1/2 sera as well as 98.5% of the sera from leptospirosis patients. The microscopic agglutination test (MAT demonstrated that rLLO antiserum had a stronger ability to agglutinate the strains of different Leptospira serovars than the rLipL32/1, rLipL21, and rOmpL1/2 antisera. More importantly, tests in hamsters showed that rLLO provided higher immunoprotective rates (91.7% than rLipL32/1, rLipL21 and rOmpL1/2 (50.0-75.0%. All the data indicate that rLLO, a recombinant fusion protein incorporating three antigens, has increased antigenicity and immunoprotective effects, and so can be used as a novel immunogen to develop a universal genetically engineered vaccine against leptospirosis.

  20. Application of Aura OMI L2G Products Compared with NASA MERRA-2 Assimilation

    Science.gov (United States)

    Zeng, Jian; Shen, Suhung; Wei, Jennifer; Johnson, James E.; Su, Jian; Meyer, David J.

    2018-01-01

    The Ozone Monitoring Instrument (OMI) is one of the instruments aboard NASA's Aura satellite. It measures ozone total column and vertical profile, aerosols, clouds, and trace gases including NO2, SO2, HCHO, BrO, and OClO using absorption in the ultraviolet electromagnetic spectrum (280 - 400 nm). OMI Level-2G (L2G) products are based on the pixel-level OMI granule satellite measurements stored within global 0.25 deg. X 0.25 deg. grids, therefore they conserve all the Level 2 (L2) spatial and temporal details for 24 hours of scientific data in one file. The second Modern-Era Retrospective analysis for Research and Applications (MERRA-2) is NASA's atmospheric reanalysis, using an upgraded version of Goddard Earth Observing System Model, version 5 (GEOS-5) data assimilation system. MERRA-2 includes aerosol data reanalysis and improved representations of stratospheric ozone, compared with its predecessor MERRA, in both instantaneous and time-averaged collections. It is found that simply comparing satellite Level-3 products might cause biases, due to lack of detailed temporal and original retrieval information. It is therefore preferable to inter-compare or implement satellite derived physical quantities directly with/to model assimilation with as high temporal and spatial resolutions as possible. This study will demonstrate utilization of OMI L2G daily aerosol and ozone products by comparing them with MERRA-2 hourly aerosol/ozone simulations, matched in both space and time aspects. Both OMI and MERRA-2 products are accessible online through NASA Goddard Earth Sciences Data Information Services Center (GES DISC, https://disc.gsfc.nasa.gov/).

  1. Identification of a new DPY19L2 mutation and a better definition of DPY19L2 deletion breakpoints leading to globozoospermia.

    Science.gov (United States)

    Ghédir, Houda; Ibala-Romdhane, Samira; Okutman, Ozlem; Viot, Géraldine; Saad, Ali; Viville, Stéphane

    2016-01-01

    The purpose of this study was to analyze DPY19L2 sequence variants to investigate the mechanism leading to the entire DPY19L2 deletion in a large cohort of infertile globozoospermic patients. An improved analysis of the DPY19L2 deletion breakpoints (BPs) allowed us to identify two BPs located in a small 1 kb region and to more precisely localize the BPs reported previously. Three genes [spermatogenesis associated 16 (SPATA16), protein interacting with PRKCA (PICK1) and DPY19L2] were previously correlated with globozoospermia, but a homozygous deletion of the entire DPY19L2 was identified as the most frequent alteration causing this phenotype. In addition, several point mutations in this gene were reported. In previous work, we have identified nine BPs for the DPY19L2 deletion clustered in two hotspot regions, while others reported a total of five BPs. We screened for the DPY19L2 deletion and for mutations in the DPY19L2, SPATA16 and PICK1 genes in a cohort of 21 Tunisian globozoospermic patients. In order to characterize the DPY19L2 deletion BPs, we sequenced a 2 kb fragment on low copy repeat (LCR) 1 and LCR2 in Tunisian fertile controls to distinguish between single-nucleotide polymorphisms (SNPs) and LCR-specific markers. Molecular analyses performed on 18 genetically independent individuals showed that 11 (61.1%) were homozygous for the DPY19L2 deletion, 2 (11.1%) were homozygous for the non-synonymous mutation (p.R298C) in exon 8, 1 patient (5.6%) was homozygous for a new splice-site mutation at the junction exon-intron 16 [c.1579_1580+4delAGGTAAinsTCAT] and no DPY19L2, SPATA16 or PICK1 mutations were identified for 4 patients (22.2%). By defining 15 specific LCR markers, we characterized 2 BPs for the DPY19L2 deletion in 11 patients showing the homozygous deletion. Using 20 non-LCR-specific SNPs, we identified 8 distinct haplotypes. A limitation of this study is the small number of patients owing to the rarity of this form of male infertility. Our data showed

  2. Carrington-L5: The UK/US Space Weather Operational Mission.

    Science.gov (United States)

    Bisi, M. M.; Trichas, M.

    2015-12-01

    Airbus Defence and Space (UK) have carried out a study for an operational L5 space weather mission, in collaboration with RAL, the UK Met Office, UCL and Imperial College London. The study looked at the user requirements for an operational mission, a model instrument payload, and a mission/spacecraft concept. A particular focus is cost effectiveness and timelineness of the data, suitable for operational forecasting needs. The study focussed on a mission at L5 assuming that a US mission to L1 will already occur, on the basis that L5 offers the greatest benefit for SWE predictions. The baseline payload has been selected to address all MOSWOC/SWPC priorities using UK/US instruments, consisting of: a heliospheric imager, coronagraph, EUV imager, magnetograph, magnetometer, solar wind analyser and radiation monitor. The platform is based on extensive re-use from Airbus' past missions to minimize the cost and a Falcon-9 launcher has been selected on the same basis. A schedule analysis shows that the earliest launch could occur in 2020, assuming Phase A KO in 2015. The study team have selected the name "Carrington" for the mission, reflecting the UK's proud history in this domain.

  3. The impact of cockpit automation on crew coordination and communication. Volume 1: Overview, LOFT evaluations, error severity, and questionnaire data

    Science.gov (United States)

    Wiener, Earl L.; Chidester, Thomas R.; Kanki, Barbara G.; Palmer, Everett A.; Curry, Renwick E.; Gregorich, Steven E.

    1991-01-01

    The purpose was to examine, jointly, cockpit automation and social processes. Automation was varied by the choice of two radically different versions of the DC-9 series aircraft, the traditional DC-9-30, and the glass cockpit derivative, the MD-88. Airline pilot volunteers flew a mission in the simulator for these aircraft. Results show that the performance differences between the crews of the two aircraft were generally small, but where there were differences, they favored the DC-9. There were no criteria on which the MD-88 crews performed better than the DC-9 crews. Furthermore, DC-9 crews rated their own workload as lower than did the MD-88 pilots. There were no significant differences between the two aircraft types with respect to the severity of errors committed during the Line-Oriented Flight Training (LOFT) flight. The attitude questionnaires provided some interesting insights, but failed to distinguish between DC-9 and MD-88 crews.

  4. L2, the minor capsid protein of papillomavirus

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Joshua W. [Department of Pathology, The Johns Hopkins University, Baltimore, MD 21287 (United States); Roden, Richard B.S., E-mail: roden@jhmi.edu [Department of Pathology, The Johns Hopkins University, Baltimore, MD 21287 (United States); Department of Oncology, The Johns Hopkins University, Baltimore, MD 21287 (United States); Department of Gynecology and Obstetrics, The Johns Hopkins University, Baltimore, MD 21287 (United States)

    2013-10-15

    The capsid protein L2 plays major roles in both papillomavirus assembly and the infectious process. While L1 forms the majority of the capsid and can self-assemble into empty virus-like particles (VLPs), L2 is a minor capsid component and lacks the capacity to form VLPs. However, L2 co-assembles with L1 into VLPs, enhancing their assembly. L2 also facilitates encapsidation of the ∼8 kbp circular and nucleosome-bound viral genome during assembly of the non-enveloped T=7d virions in the nucleus of terminally differentiated epithelial cells, although, like L1, L2 is not detectably expressed in infected basal cells. With respect to infection, L2 is not required for particles to bind to and enter cells. However L2 must be cleaved by furin for endosome escape. L2 then travels with the viral genome to the nucleus, wherein it accumulates at ND-10 domains. Here, we provide an overview of the biology of L2. - Highlights: • L2 is the minor antigen of the non-enveloped T=7d icosahedral Papillomavirus capsid. • L2 is a nuclear protein that can traffic to ND-10 and facilitate genome encapsidation. • L2 is critical for infection and must be cleaved by furin. • L2 is a broadly protective vaccine antigen recognized by neutralizing antibodies.

  5. L2, the minor capsid protein of papillomavirus

    International Nuclear Information System (INIS)

    Wang, Joshua W.; Roden, Richard B.S.

    2013-01-01

    The capsid protein L2 plays major roles in both papillomavirus assembly and the infectious process. While L1 forms the majority of the capsid and can self-assemble into empty virus-like particles (VLPs), L2 is a minor capsid component and lacks the capacity to form VLPs. However, L2 co-assembles with L1 into VLPs, enhancing their assembly. L2 also facilitates encapsidation of the ∼8 kbp circular and nucleosome-bound viral genome during assembly of the non-enveloped T=7d virions in the nucleus of terminally differentiated epithelial cells, although, like L1, L2 is not detectably expressed in infected basal cells. With respect to infection, L2 is not required for particles to bind to and enter cells. However L2 must be cleaved by furin for endosome escape. L2 then travels with the viral genome to the nucleus, wherein it accumulates at ND-10 domains. Here, we provide an overview of the biology of L2. - Highlights: • L2 is the minor antigen of the non-enveloped T=7d icosahedral Papillomavirus capsid. • L2 is a nuclear protein that can traffic to ND-10 and facilitate genome encapsidation. • L2 is critical for infection and must be cleaved by furin. • L2 is a broadly protective vaccine antigen recognized by neutralizing antibodies

  6. A new characterization of L2(p) by NSE

    Indian Academy of Sciences (India)

    In this paper we give a new characterization of simple group L2(p) with ... Introduction. Throughout this paper, all groups are finite and G denotes a group. .... [5] Mazurov V D and Khukhro E I, Unsolved Problems in Group Theory, the Kourovka.

  7. XAS study of V2O5/Al2O3 catalysts doped with rare earth oxides

    International Nuclear Information System (INIS)

    Centeno, M.A.; Malet, P.; Capitan, M.J.; Benitez, J.J.; Carrizosa, I.; Odriozola, J.A.

    1995-01-01

    This paper reports on XAS studies of well dispersed V 2 O 5 /Al 2 O 3 and V 2 O 5 /Sm 2 O 3 /Al 2 O 3 samples. XAS spectra at V-K and Sm-L III edges show that the rare earth oxide favours the formation of regular tetrahedral units, [VO 4 ], over the surface of the support. Positions of the preedge peak at the V-K edge, and intensities of the white line at the Sm-L III edge also suggest modifications in the electronic density around V and Sm atoms when they are simultaneously supported over Al 2 O 3 . ((orig.))

  8. Fatal intoxication with 3-methyl-N-methylcathinone (3-MMC) and 5-(2-aminopropyl)benzofuran (5-APB).

    Science.gov (United States)

    Adamowicz, Piotr; Zuba, Dariusz; Byrska, Bogumiła

    2014-12-01

    The emergence of a large number of new psychoactive substances (NPSs) in recent years poses a serious problem to clinical and forensic toxicologists. Here we report a patient who administrated ca. 500mg of 3-MMC (3-methyl-N-methylcathinone) and 400mg of 5-APB (5-(2-aminopropyl)benzofuran) in combination with 80g of ethyl alcohol. The clinical manifestations included agitation, seizures, hypertension, tachycardia, hyperthermia and bradycardia. The patient did not recover and died around 4h after the use of drugs. The cause of death was acute cardiovascular collapse that occurred following mixed intoxication with NPSs and alcohol. Toxicological analysis of post-mortem blood revealed 3-MMC and 5-APB in concentrations of 1.6μg/mL and 5.6μg/mL, respectively. Moreover, the serum alcohol concentration was 1.4g/L in ante-mortem sample collected 1h after admission to the hospital. This is the first report on blood concentration of 3-MMC and 5-APB in fatal intoxication. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  9. Phase I study of 6-diazo-5-oxo-L-norleucine (DON).

    Science.gov (United States)

    Sklaroff, R B; Casper, E S; Magill, G B; Young, C W

    1980-01-01

    We conducted a phase I study of 6-diazo-5-oxo-L-norleucine given iv on a twice weekly schedule. Twenty-six evaluable patients received 31 courses of the drug. Doses ranged from 100 to 500 mg/m2. Nausea with vomiting was the dose-limiting toxic effect, transient thrombocytopenia was seen frequently, and mucositis occurred in 39% of the patients. No definite therapeutic responses were observed in 18 patients with measurable lesions. The recommended dose for phase II studies is 200-300 mg/m2 iv twice weekly.

  10. Effects of 3,5-diiodo-L-thyronine on browning of white adipose tissue%3,5-二碘-L-甲状腺素对白色脂肪棕色化的作用

    Institute of Scientific and Technical Information of China (English)

    柯孟婷; 孙家忠; 李扬

    2016-01-01

    目的 探究3,5-二碘-L-甲状腺素(T2)诱导白色脂肪棕色化的作用.方法 3T3-LI脂肪前体细胞诱导分化并用油红O染色进行鉴定;细胞分化过程中予不同浓度梯度(1 nmol/L、10 nmol/L、100 nmol/L)的T2处理,待细胞分化成熟后,分别用实时荧光定量PCR、Western印迹检测解耦联蛋白-1(UCP-1)表达水平的变化;仅予高浓度(100 nmol/L) T2处理,Western印迹法检测其他棕色脂肪功能性基因,包括诱导细胞死亡DNA片段化因子α样效应因子A(CIDEA)、过氧化物酶体增殖物活化受体γ协同刺激因子-1α(PGC-1α)蛋白表达变化.结果 3T3-L1脂肪前体细胞呈成纤维细胞样形态,胞浆中无脂滴;诱导分化成熟后光镜下油红O染色可见细胞内大量环状脂滴.在细胞分化过程中予不同浓度T2干预后,分化成熟的脂肪细胞上UCP-1 mRNA水平均有升高(t=3.97、11.77、17.7,P均<0.05),蛋白表达水平也均有改变(t=13.31、14.55、23.62,P均<0.05),且在高浓度(100nmoL/L)下最明显.在高浓度T2(100 nmol/L)干预下,成熟脂肪细胞的棕色脂肪其他功能性基因蛋白CIDEA、PGC-1α水平表达增加(t=15.92、17.36,P均<0.05).结论 T2可诱导由3T3-L1脂肪前体细胞分化而来的成熟白色脂肪细胞表达棕色脂肪功能性基因.%Objective To explore the effects of 3,5-diiodo-L-thyronine(T2) on browning of white adipose tissue.Methods Differentiation of 3T3-L1 adipose precursor cells were induced and identified by oil red O staining.During the differentiation process,3T3-L1 were treated by different concentrations of T2 (1 nmol/L,10 nmol/L,100 nmol/L).After the maturation of 3T3-L1,the level of uncoupling protein-1 (UCP-1) mRNA and protein were detected by RT-PCR and Western blotting,respectively.Specific proteins of brown adipose tissue including cell death-inducing DNA fragmentation factor alpha like effector A (CIDEA) and peroxisome proliferator-activated receptor γ coactivator-1 α (PGC-1 α) were

  11. Digital expression profiling identifies RUNX2, CDC5L, MDM2, RECQL4, and CDK4 as potential predictive biomarkers for neo-adjuvant chemotherapy response in paediatric osteosarcoma.

    Directory of Open Access Journals (Sweden)

    Jeffrey W Martin

    Full Text Available Osteosarcoma is the most common malignancy of bone, and occurs most frequently in children and adolescents. Currently, the most reliable technique for determining a patients' prognosis is measurement of histopathologic tumor necrosis following pre-operative neo-adjuvant chemotherapy. Unfavourable prognosis is indicated by less than 90% estimated necrosis of the tumor. Neither genetic testing nor molecular biomarkers for diagnosis and prognosis have been described for osteosarcomas. We used the novel nanoString mRNA digital expression analysis system to analyse gene expression in 32 patients with sporadic paediatric osteosarcoma. This system used specific molecular barcodes to quantify expression of a set of 17 genes associated with osteosarcoma tumorigenesis. Five genes, from this panel, which encoded the bone differentiation regulator RUNX2, the cell cycle regulator CDC5L, the TP53 transcriptional inactivator MDM2, the DNA helicase RECQL4, and the cyclin-dependent kinase gene CDK4, were differentially expressed in tumors that responded poorly to neo-adjuvant chemotherapy. Analysis of the signalling relationships of these genes, as well as other expression markers of osteosarcoma, indicated that gene networks linked to RB1, TP53, PI3K, PTEN/Akt, myc and RECQL4 are associated with osteosarcoma. The discovery of these networks provides a basis for further experimental studies of role of the five genes (RUNX2, CDC5L, MDM2, RECQL4, and CDK4 in differential response to chemotherapy.

  12. Co-occurrence of Mycoplasma Species and Pigeon Herpesvirus-1 Infection in Racing Pigeons ( Columba livia).

    Science.gov (United States)

    Hellebuyck, Tom; Göbel, Stephan; Pasmans, Frank; Adriaensen, Connie; Martel, An

    2017-12-01

    Oropharyngeal swab samples were collected from 438 live racing pigeons ( Columba livia), with and without signs of respiratory disease, that were housed in 220 lofts in 3 provinces in the western part of the Netherlands. Polymerase chain reaction (PCR) was used to identify Mycoplasma species and pigeon herpesvirus-1 (PHV-1) from the samples. In 8.6% of the pigeon lofts tested, signs of respiratory disease were present in pigeons at sampling, and in 30.9% of the sampled pigeon lofts, respiratory signs were observed in pigeons during the 6-month period immediately before sampling. A total of 39.8% of tested pigeons (54.5% of tested lofts) were positive for Mycoplasma species, and 30.6% of tested pigeons (48.6% of tested lofts) were positive for PHV-1. In 15.8% of the tested pigeons (26.8% of tested pigeon lofts), coinfection by Mycoplasma species and PHV-1 was identified. The number of pigeon lofts having pigeons coinfected by Mycoplasma species and PHV-1 was higher than that where only one of the infections was identified. Neither the presence of Mycoplasma species, PHV-1, nor the co-occurrence of both infections was significantly associated with signs of respiratory disease.

  13. L1 effects on the processing of grammatical gender in L2

    NARCIS (Netherlands)

    Sabourin, L; Foster-Cohen, S.; Nizegorodcew, A.

    2001-01-01

    This paper explores L1 effects on the L2 off-line processing of Dutch (grammatical gender) agreement The L2 participants had either German, English or a Romance language as their L1. Non-gender agreement (finiteness and agreement) was tested to ascertain the level of proficiency of the participants

  14. Dosage de l'acide nitro-5 furylacrylique dans les vins blancs (1

    Directory of Open Access Journals (Sweden)

    Suzanne Lafon-Lafourcade

    1975-09-01

    Rappelons que l'acide nitro-5 furylacrylique est une substance jaune, inodore et sans saveur, stable à la lumière et à la chaleur. Ses sels de sodium et de potassium sont très solubles dans l'eau, dans l'alcool et le méthanol, mais insolubles dans les solvants non miscibles à l'eau. Le 5-NFA ne distille pas et n'est pas entraînable par la vapeur d'eau. (1 Travail réalisé en collaboration avec le Conseil Interprofessionnel du Vin de Bordeaux.

  15. L4-L5-S1 human dermatomes: a clinical, electromyographical, imaging and surgical findings Dermátomos humanos L4, L5 e S1: achados clínicos, eletromiográficos, de imagem e cirúrgicos

    Directory of Open Access Journals (Sweden)

    Antonio Tadeu de Souza Faleiros

    2009-06-01

    Full Text Available There is substantial controversy in literature about human dermatomes. We studied L4, L5, and S1 inferior limb dermatomes by comparing clinical signs and symptoms with conduction studies, electromyographical data, neurosurgical findings, and imaging data from computerized tomography (CT or magnetic resonance imaging (MRI. After analyzing 60 patients, we concluded that L4 is probably located in the medial aspect of the leg, L5 in the lateral aspect of the leg and foot dorsus, and S1 in the posterior aspect of the backside, tight, leg and plantar foot skin. This is the first time that these human dermatomes have been evaluated by combined analysis of clinical, electromyographical, neurosurgical, and imaging data.Há controvérsia na literatura sobre os dermátomos humanos. Estudamos dermátomos do membro inferior comparando sinais e sintomas com estudos eletromiográficos, de imagem e achados cirúrgicos. Analisando 60 pacientes, concluímos que o dermátomo L4 provavelmente está localizado na região medial da perna, o dermátomo L5 na região lateral da perna e dorso do pé, e o dermátomo S1 na nádega, região posterior da coxa e da perna e na região plantar. Este é o primeiro estudo que os dermátomos do membro inferior foram analisados através de dados clínicos, eletromiográficos, imagem e achados cirúrgicos.

  16. 3,5 Diiodo-L-Thyronine (T2 Does Not Prevent Hepatic Steatosis or Insulin Resistance in Fat-Fed Sprague Dawley Rats.

    Directory of Open Access Journals (Sweden)

    Daniel F Vatner

    Full Text Available Thyroid hormone mimetics are alluring potential therapies for diseases like dyslipidemia, nonalcoholic fatty liver disease (NAFLD, and insulin resistance. Though diiodothyronines are thought inactive, pharmacologic treatment with 3,5- Diiodo-L-Thyronine (T2 reportedly reduces hepatic lipid content and improves glucose tolerance in fat-fed male rats. To test this, male Sprague Dawley rats fed a safflower-oil based high-fat diet were treated with T2 (0.25 mg/kg-d or vehicle. Neither 10 nor 30 days of T2 treatment had an effect on weight, adiposity, plasma fatty acids, or hepatic steatosis. Insulin action was quantified in vivo by a hyperinsulinemic-euglycemic clamp. T2 did not alter fasting plasma glucose or insulin concentration. Basal endogenous glucose production (EGP rate was unchanged. During the clamp, there was no difference in insulin stimulated whole body glucose disposal. Insulin suppressed EGP by 60% ± 10 in T2-treated rats as compared with 47% ± 4 suppression in the vehicle group (p = 0.32. This was associated with an improvement in hepatic insulin signaling; insulin stimulated Akt phosphorylation was ~2.5 fold greater in the T2-treated group as compared with the vehicle-treated group (p = 0.003. There was no change in expression of genes thought to mediate the effect of T2 on hepatic metabolism, including genes that regulate hepatic lipid oxidation (ppara, carnitine palmitoyltransferase 1a, genes that regulate hepatic fatty acid synthesis (srebp1c, acetyl coa carboxylase, fatty acid synthase, and genes involved in glycolysis and gluconeogenesis (L-pyruvate kinase, glucose 6 phosphatase. Therefore, in contrast with previous reports, in Sprague Dawley rats fed an unsaturated fat diet, T2 administration failed to improve NAFLD or whole body insulin sensitivity. Though there was a modest improvement in hepatic insulin signaling, this was not associated with significant differences in hepatic insulin action. Further study will be

  17. L1 and L2 Spoken Word Processing: Evidence from Divided Attention Paradigm.

    Science.gov (United States)

    Shafiee Nahrkhalaji, Saeedeh; Lotfi, Ahmad Reza; Koosha, Mansour

    2016-10-01

    The present study aims to reveal some facts concerning first language (L 1 ) and second language (L 2 ) spoken-word processing in unbalanced proficient bilinguals using behavioral measures. The intention here is to examine the effects of auditory repetition word priming and semantic priming in first and second languages of these bilinguals. The other goal is to explore the effects of attention manipulation on implicit retrieval of perceptual and conceptual properties of spoken L 1 and L 2 words. In so doing, the participants performed auditory word priming and semantic priming as memory tests in their L 1 and L 2 . In a half of the trials of each experiment, they carried out the memory test while simultaneously performing a secondary task in visual modality. The results revealed that effects of auditory word priming and semantic priming were present when participants processed L 1 and L 2 words in full attention condition. Attention manipulation could reduce priming magnitude in both experiments in L 2 . Moreover, L 2 word retrieval increases the reaction times and reduces accuracy on the simultaneous secondary task to protect its own accuracy and speed.

  18. Event-related brain potentials and second language learning: syntactic processing in late L2 learners at different L2 proficiency levels

    NARCIS (Netherlands)

    Hell, J.G. van; Tokowicz, N.

    2010-01-01

    There are several major questions in the literature on late second language (L2) learning and processing. Some of these questions include: Can late L2 learners process an L2 in a native-like way? What is the nature of the differences in L2 processing among L2 learners at different levels of L2

  19. L2 writing and L2 written feedback in upper secondary schools as experienced by teachers

    OpenAIRE

    Manousou, Angeliki

    2015-01-01

    L2 written feedback is a multi-faceted issue and this is the reason behind the big number of studies that have been conducted on it. However, the majority of studies deal with learners’ opinions of teachers’ feedback or several types of feedback and their advantages and disadvantages. There are no studies that could have addressed teachers’ opinions of their L2 written feedback. This study attempts to describe how L2 teachers view their written feedback on learners’ essays. ...

  20. Stability at Potential Maxima: The L-4 and L-5 Points of the Restricted Three-Body Problem.

    Science.gov (United States)

    Greenberg, Richard; Davis, Donald R.

    1978-01-01

    Describes a dynamical system which is stable at potential maxima. The maxima, called L-4 and L-5, are stable locations of the restricted three-body problem. Energy loss from the system will tend to drive it away from stability. (GA)

  1. Aqp5 is a new transcriptional target of Dot1a and a regulator of Aqp2.

    Directory of Open Access Journals (Sweden)

    Hongyu Wu

    Full Text Available Dot1l encodes histone H3 K79 methyltransferase Dot1a. Mice with Dot1l deficiency in renal Aqp2-expressing cells (Dot1l(AC develop polyuria by unknown mechanisms. Here, we report that Aqp5 links Dot1l deletion to polyuria through Aqp2. cDNA array analysis revealed and real-time RT-qPCR validated Aqp5 as the most upregulated gene in Dot1l(AC vs. control mice. Aqp5 protein is barely detectable in controls, but robustly expressed in the Dot1l(AC kidneys, where it colocalizes with Aqp2. The upregulation of Aqp5 is coupled with reduced association of Dot1a and H3 dimethyl K79 with specific subregions in Aqp5 5' flanking region in Dot1l(AC vs. control mice. In vitro studies in IMCD3, MLE-15 and 293Tcells using multiple approaches including real-time RT-qPCR, luciferase reporter assay, cell surface biotinylation assay, colocalization, and co-immunoprecipitation uncovered that Dot1a represses Aqp5. Human AQP5 interacts with AQP2 and impairs its cell surface localization. The AQP5/AQP2 complex partially resides in the ER/Golgi. Consistently, AQP5 is expressed in none of 15 normal controls, but in all of 17 kidney biopsies from patients with diabetic nephropathy. In the patients with diabetic nephropathy, AQP5 colocalizes with AQP2 in the perinuclear region and AQP5 expression is associated with impaired cellular H3 dimethyl K79. Taken together, these data for the first time identify Aqp5 as a Dot1a potential transcriptional target, and an Aqp2 binding partner and regulator, and suggest that the upregulated Aqp5 may contribute to polyuria, possibly by impairing Aqp2 membrane localization, in Dot1l(AC mice and in patients with diabetic nephropathy.

  2. The coercivity mechanism of sintered SM(Co_b_a_lFe_0_._2_4_5Cu_0_._0_7Zr_0_._0_2)_7_._8 permanent magnets with different isothermal annealing time

    International Nuclear Information System (INIS)

    Sun, Wei; Zhu, Minggang; Guo, Zhaohui; Fang, Yikun; Li, Wei

    2015-01-01

    Precipitation-hardened 2:17-type SmCo permanent magnet has attracted much attention due to its high Curie temperature and excellent magnetic properties. Sm(Co_b_a_lFe_0_._2_4_5Cu_0_._0_7Zr_0_._0_2)_7_._8 (at%) sintered magnets with high remanence (B_r ~1.15 T) were prepared using a traditional powder metallurgy method. The intrinsic coercivity H_c_j of the magnets was increased from 429 to 994 kA m"−"1 with isothermal annealing time increasing from 10 to 40 h, which is different from the phenomenon that increasing aging time leads to a reduced coercivity mentioned in the Ref. [16]. In consideration of rarely report about the microstructure of the final magnet isothermally annealed for 40 h, we have tried to originally analyze the relationship between the microstructure and the magnetic properties. Besides, the lattice constants of sintered Sm(Co_b_a_lFe_0_._2_4_5Cu_0_._0_7Zr_0_._0_2)_7_._8 permanent magnet isothermally annealed for 40 h have been given by indexing the HRTEM results including the selected area electron diffraction (SAED) and HRTEM images.

  3. Impact of Wines and Wine Constituents on Cyclooxygenase-1, Cyclooxygenase-2, and 5-Lipoxygenase Catalytic Activity

    Directory of Open Access Journals (Sweden)

    Zsofia Kutil

    2014-01-01

    Full Text Available Cyclooxygenases and lipoxygenases are proinflammatory enzymes; the former affects platelet aggregation, vasoconstriction, vasodilatation and later the development of atherosclerosis. Red wines from Georgia and central and western Europe inhibited cyclooxygenase-1 (COX-1 activity in the range of 63–94%, cyclooxygenase-2 (COX-2 activity in the range of 20–44% (tested at a concentration of 5 mL/L, and 5-lipoxygenase (5-LOX activity in the range of 72–84% (at a concentration of 18.87 mL/L. White wines inhibited 5-LOX in the range of 41–68% at a concentration of 18.87 mL/L and did not inhibit COX-1 and COX-2. Piceatannol (IC50 = 0.76 μM was identified as a strong inhibitor of 5-LOX followed by luteolin (IC50 = 2.25 μM, quercetin (IC50 = 3.29 μM, and myricetin (IC50 = 4.02 μM. trans-Resveratrol was identified as an inhibitor of COX-1 (IC50 = 2.27 μM and COX-2 (IC50 = 3.40 μM. Red wine as a complex mixture is a powerful inhibitor of COX-1, COX-2, and 5-LOX, the enzymes involved in eicosanoid biosynthetic pathway.

  4. Stereoselective chemoenzymatic synthesis of the four stereoisomers of l-2-(2-carboxycyclobutyl)glycine and pharmacological characterization at human excitatory amino acid transporter subtypes 1, 2, and 3

    DEFF Research Database (Denmark)

    Faure, Sophie; Jensen, Anders A.; Maurat, Vincent

    2006-01-01

    The four stereoisomers of l-2-(2-carboxycyclobutyl)glycine, l-CBG-I, l-CBG-II, l-CBG-III, and l-CBG-IV, were synthesized in good yield and high enantiomeric excess, from the corresponding cis and trans-2-oxalylcyclobutanecarboxylic acids 5 and 6 using the enzymes aspartate aminotransferase (AAT......) and branched chain aminotransferase (BCAT) from Escherichia coli. The four stereoisomeric compounds were evaluated as potential ligands for the human excitatory amino acid transporters, subtypes 1, 2, and 3 (EAAT1, EAAT2, and EAAT3) in the FLIPR membrane potential assay. While the one trans-stereoisomer, l...

  5. Tule paistel : kaminast ahjuni, lõõrist korstnani / Piret Veigel

    Index Scriptorium Estoniae

    Veigel, Piret, 1961-

    2005-01-01

    Priit Hamer firmast Agabus, Endjärv & Truverk Arhitektid vastuseks lugejate küsimustele kamina ja küttesüsteemi valikust, tõmbeprobleemidest, avatud, disain- ja küttekaminast, ahjudest, valmiskaminatest, küttepuudest, tuleohutusest. 6 värv. ill.: Andrus Tuisu projekteeritud kamin loft-korteris jm.

  6. A refined gravity model from Lageos /GEM-L2/

    Science.gov (United States)

    Lerch, F. J.; Klosko, S. M.; Patel, G. B.

    1982-01-01

    Lageos satellite laser ranging (SLR) data taken over a 2.5 yr period were employed to develop the Goddard Earth Model GEM-L2, a refined gravity field model. Additional data was gathered with 30 other satellites, resulting in spherical harmonics through degree and order 20, based on over 600,000 measurements. The Lageos data was accurate down to 10 cm, after which the GEM 9 data were used to make adjustments past order 7. The resolution of long wavelength activity, through degree and order 4, was made possible by the Lageos data. The GEM-L2 model features a 20 x 20 geopotential, tracking station coordinates (20), 5-day polar motion and A1-UT1 values, and a GM value of 398,600.607 cu km/sq sec. The accuracy of station positioning has been raised to within 6 cm total position globally and within 1.8 cm in baselines. It is concluded that SLR is useful for measuring tectonic plate motions and inter-plate deformations.

  7. Upper limits to the detection of ammonia from protoplanetary disks around HL Tauri and L1551-IRS 5

    Science.gov (United States)

    Gomez, Jose F.; Torrelles, Jose M.; Ho, Paul T. P.; Rodriguez, Luis F.; Canto, Jorge

    1993-01-01

    We present NH3(1, 1) and (2, 2) observations of the young stellar sources HL Tau and L1551-IRS 5 using the VLA in its B-configuration, which provides an angular resolution of about 0.4 arcsec (about 50 AU at 140 pc) at 1.3 cm wavelength. Our goal was to detect and resolve circumstellar molecular disks with radius of the order of 100 AU around these two sources. No ammonia emission was detected toward either of them. The 3-sigma levels were 2.7 mJy/beam and 3.9 mJy/beam for HL Tau and L1551-IRS 5, respectively, with a velocity resolution of about 5 km/s. With this nondetection, we estimate upper limits to the mass of the proposed protoplanetary molecular disks (within a radius of 10 AU from the central stars) on the order of 0.02/(X(NH3)/10 exp -8) solar mass for HL Tau and 0.1/(X(NH3)/10 exp -8) solar mass for L1551-IRS 5.

  8. Alteração do teor de cálcio no banho de DP para 2,5 mEq/L é eficaz no reestabelecimento dos valores preconizados por diretrizes atuais em pacientes com PTH < 150 pg/dL Low-calcium peritoneal dialysis solution is effective in bringing PTH levels to the range recommended by current guidelines in patients with PTH levels < 150 pg/dL

    Directory of Open Access Journals (Sweden)

    Thyago Proença de Moraes

    2010-09-01

    Full Text Available INTRODUÇÃO/OBJETIVO: A doença óssea adinâmica (DOA é um achado comum em diálise peritoneal (PD e é considerada fator de risco para desenvolvimento de fraturas e doença cardiovascular. Dados do BRAZPD apontam as soluções de cálcio a 3,5 mEq/L presentes na maioria das prescrições no país, que possui quase 9.000 pacientes em PD. É comum o balanço positivo de cálcio com concentrações a 3,5 mEq/L contribuindo para o desenvolvimento de DOA. Diretrizes atuais recomendam um PTHi na DRC V em diálise entre 2 e 9 vezes (150-500 pg/mL o valor máximo da normalidade. O objetivo deste estudo foi avaliar a resposta em 6 meses do PTH-i após a conversão para solução de cálcio a 2,5 mEq/L de pacientes que usavam soluções com cálcio a 3,5 mEq/L e com PTH-i basal INTRODUCTION/OBJECTIVE: Adinamic bone disease (ABD is a common finding in peritoneal dialysis (PD and is associated with higher risk of developing cardiovascular and bone disease. Data from BRAZPD indicates that 3.5 mEq/L calcium PD solutions represents the majority of PD prescriptions in the country. A positive calcium balance can contribute to ABD development. Currently guidelines suggest that PTH-i levels in end stage renal disease should be kept from 150-300 pg/mL. The purpose of this study is to evaluate 6 month PTH-i response after conversion to 2.5 mEq/L calcium PD solution in patients with baseline PTH-i levels < 150 pg/mL. METHODS: Prospective, observational study of all prevalent patients (at least 90 days on therapy on PD of a single Brazilian center from January 2008 to May 2009. Inclusion criteria (1 be in use of a PD solution with 3.5mEq/L of calcium; (2 baseline PTH leves < 150 pg/ mL. According to clinical practice patients could be switched to PD solutions with 2.5 mEq/L of calcium. RESULTS: 35 patients (age 62 ± 17 years were included. Of these 22 were converted to 2.5 mEq/L calcium solutions. Diabetic nephropathy (36% was the main cause of renal disease

  9. Enthalpies of ligand substitution for [Mo(η5C5H5)(CO)2(NO)] – The role of π-bonding effects in metal–ligand bond strengths

    International Nuclear Information System (INIS)

    Majumdar, Subhojit; Captain, Burjor; Cazin, Catherine S.J.; Nolan, Steven P.; Hoff, Carl D.

    2014-01-01

    Graphical abstract: - Highlights: • Enthalpies of ligand substitution are measured for Mo(C 5 H 5 )(CO) 2 (NO). • Phosphines and N-heterocyclic carbenes are stronger ligands and displace CO. • Backbonding to π ∗ orbitals is an important part of complex stability. • FTIR studies show shifts to lower wavenumbers of ν-CO and ν-NO. • Structural studies show lengthening of the C-O and N-O bonds. - Abstract: Enthalpies of ligand substitution for [Mo(η 5 -C 5 H 5 )(CO) 2 (NO)] producing [Mo(η 5 -C 5 H 5 )Mo(CO)(L)(NO)] have been measured by solution calorimetry at 30 °C in THF for L = P(OMe) 3 2 2 Ph 3 (SIPr = 1,3-bis(2,6-bis(diisopropylphenyl)imidazolinylidene; IPr = 1,3-bis(2,6-bis(diisopropylphenyl)-imidazol-2-ylidene)). The accepting metal fragment [Mo(η 5 -C 5 H 5 )(CO)(NO)] has a vacant site containing strongly π-accepting carbonyl and nitrosyl ligands and this is shown to influence the stability of the product complex. Infrared studies of both ν CO and ν NO show that metal-to-ligand backbonding increases in the order P(OMe) 3 3 5 -C 5 H 5 )(CO)(IPr)(NO)] and [Mo(η 5 -C 5 H 5 )(CO)(SIPr)(NO)] are reported

  10. Synthesis and analytics of 2,2,3,4,4-d5-19-nor-5alpha-androsterone--an internal standard in doping analysis.

    Science.gov (United States)

    Gaertner, Peter; Bica, Katharina; Felzmann, Wolfgang; Forsdahl, Guro; Gmeiner, Günter

    2007-05-01

    A short and efficient synthesis of pentadeuterated 2,2,3,4,4-d5-19-nor-5alpha-androsterone 7 starting from 19-norandrost-4-ene-3,17-dione 1 by a d1-L-Selectride mediated stereo- and regioselective reduction of the 3-keto group is presented. The use of compound 7 as internal standard for the detection of anabolic steroids via mass spectrometric techniques such as gas chromatography-mass spectrometry (GC-MS) is discussed.

  11. Trailing (L5) Neptune Trojans: 2004 KV18 and 2008 LC18

    International Nuclear Information System (INIS)

    Guan Pu; Zhou Liyong; Li Jian

    2012-01-01

    The population of Neptune Trojans is believed to be bigger than that of Jupiter Trojans and that of asteroids in the main belt, although only eight members of this distant asteroid swarm have been observed up to now. Six leading Neptune Trojans around the Lagrange point L 4 discovered earlier have been studied in detail, but two trailing ones found recently around the L 5 point, 2004 KV18 and 2008 LC18, have not yet been investigated. We report our investigations on the dynamical behaviors of these two new Neptune Trojans. Our calculations show that the asteroid 2004 KV18 is a temporary Neptune Trojan. Most probably, it was captured into the trailing Trojan cloud no earlier than 2.03 × 10 5 yr ago, and it will not maintain this position later than 1.65 × 10 5 yr in the future. Based on the statistics from our orbital simulations, we argue that this object is more like a scattered Kuiper belt object. By contrast, the orbit of 2008 LC18 is much more stable. Among the clone orbits spreading within the orbital uncertainties, a considerable portion of clones may survive on the L 5 tadpole orbits for 4 Gyr. The strong dependence of the stability on the semimajor axis and resonant angle suggests that further observations are badly required to constrain the orbit in the stable region. We also discuss the implications of the existence and dynamics of these two trailing Trojans over the history of the solar system.

  12. A systematic review on aromatic L-amino acid decarboxylase (5-hydroxytryptophan decarboxylase)

    International Nuclear Information System (INIS)

    Rahman, M.K.; Nagatsu, T.

    1988-11-01

    Aromatic L-amino acid decarboxylase (AADC, EC. 4.1.1.28) with L-5-hydroxytryptophan as a substrate (also called L-5-hydroxytryptophan decarboxylase, 5-HTPDC) decarboxylates L-5-hydroxytryptophan to serotonin (5-HT), an important neurotransmitter that involved in the regulation of neuronal functions, behaviour and emotion of higher animals. As it is an important enzyme, many researchers are now working on its physiological functions and properties and also on its isolation, purification and characterization from mammalian tissues. But up to now no systematic review studies have been done on this enzyme. We made systematic studies on this enzyme in tissues and brains of rats, and human subjects. We also developed highly sensitive assay methods of the enzyme. This new method led us to discover the enzyme in the sera of various animals. We examined the developmental changes of 5-HTPDC in the sera of animals. We discovered an endogenous inhibitor of the enzyme in the monkey blood. The purification of the enzyme were performed by us and other researches from the sera, brains, adrenals, liver and kidneys of mammals. These and other results of up to date research papers on 5-HTPDC have been reviewed in this paper. (author). 71 refs, 10 figs, 14 tabs

  13. An overview of L-2-hydroxyglutarate dehydrogenase gene (L2HGDH) variants: a genotype-phenotype study

    DEFF Research Database (Denmark)

    Steenweg, Marjan E; Jakobs, Cornelis; Errami, Abdellatif

    2010-01-01

    L-2-Hydroxyglutaric aciduria (L2HGA) is a rare, neurometabolic disorder with an autosomal recessive mode of inheritance. Affected individuals only have neurological manifestations, including psychomotor retardation, cerebellar ataxia, and more variably macrocephaly, or epilepsy. The diagnosis of ...

  14. Perpendicular magnetic anisotropy in Co2FeAl0.5Si0.5/MgO bottom electrodes for magnetic tunnel junctions

    International Nuclear Information System (INIS)

    Li, X.Q.; Wu, Y.; Gao, S.; Xu, X.G.; Miao, J.; Jiang, Y.

    2013-01-01

    Perpendicular magnetic anisotropy (PMA) was achieved in annealed Co 2 FeAl 0.5 Si 0.5 (CFAS)/MgO-based multilayers with good thermal stability up to 400 °C and a large anisotropy energy density K u over 2.0 × 10 5 J/m 3 . The thickness of the full-Heusler CFAS film to maintain PMA is up to 4.8 nm in which the co-existence of disordered A2, ordered B2 and fully ordered L2 1 structures is observed. X-ray photoelectron spectroscopy analysis demonstrates that the origin of the PMA is the hybridization between Co 3d and O 2p orbitals at the CFAS/MgO interface. - Highlights: • We achieved perpendicular magnetic anisotropy (PMA) in Co 2 FeAl 0.5 Si 0.5 (4.8 nm) film; • L2 1 , B2 and A2 phases coexist in perpendicular magnetic anisotropic Co 2 FeAl 0.5 Si 0.5 ; • Magnetic properties have strong dependence on the annealing temperature; • The PMA is induced by the hybridization between Co-3d and O-2p orbitals

  15. GLI ERRORI DI ITALIANO L1 ED L2: INTERFERENZA E APPRENDIMENTO

    Directory of Open Access Journals (Sweden)

    Rosaria Solarino

    2011-02-01

    Full Text Available Si può oggi affrontare il tema degli errori di italiano da una prospettiva che possa giovare contemporaneamente a docenti di italiano L1 ed L2? Noi pensiamo di sì: la ricerca glottodidattica sembra aver ormai apprestato un terreno comune alle due situazioni di apprendimento, sgombrando il campo da vecchi pregiudizi e distinzioni che appaiono ormai superate. Attraverso la contrapposizione di concetti quali “lingua parlata/lingua scritta”,  “errori di lingua / errori di linguaggio”, “apprendimento spontaneo/apprendimento guidato”, “italiano L1/italiano L2”, “errori di apprendimento/errori di interferenza, si indicano diversi criteri per la interpretazione degli errori e la loro valutazione in relazione alle cause, alle situazioni comunicative, ai contesti o allo stadio di evoluzione dell’apprendimento della lingua.     Errors in italian L1 and L2: interference and learning   Can errors in Italian be approached in a way that benefits both L1 and L2 Italian teachers? We believe so: glottodidactic research seems to have prepared a common terrain for these two learning situations, clearing the field of old prejudices and obsolete distinctions.  Through the juxtaposition of concepts like “spoken language/written language”, “language errors/speech errors”, “spontaneous learning/guided learning”, “L1 Italian/L2 Italian”, “learning errors/interference errors”, different criteria for interpreting errors and evaluating them in relation to their causes, to communicative situations, to contexts and the developmental state in learning a language are singled out.

  16. How Does L1 and L2 Exposure Impact L1 Performance in Bilingual Children? Evidence from Polish-English Migrants to the United Kingdom

    Directory of Open Access Journals (Sweden)

    Ewa Haman

    2017-09-01

    Full Text Available Most studies on bilingual language development focus on children’s second language (L2. Here, we investigated first language (L1 development of Polish-English early migrant bilinguals in four domains: vocabulary, grammar, phonological processing, and discourse. We first compared Polish language skills between bilinguals and their Polish non-migrant monolingual peers, and then investigated the influence of the cumulative exposure to L1 and L2 on bilinguals’ performance. We then examined whether high exposure to L1 could possibly minimize the gap between monolinguals and bilinguals. We analyzed data from 233 typically developing children (88 bilingual and 145 monolingual aged 4;0 to 7;5 (years;months on six language measures in Polish: receptive vocabulary, productive vocabulary, receptive grammar, productive grammar (sentence repetition, phonological processing (non-word repetition, and discourse abilities (narration. Information about language exposure was obtained via parental questionnaires. For each language task, we analyzed the data from the subsample of bilinguals who had completed all the tasks in question and from monolinguals matched one-on-one to the bilingual group on age, SES (measured by years of mother’s education, gender, non-verbal IQ, and short-term memory. The bilingual children scored lower than monolinguals in all language domains, except discourse. The group differences were more pronounced on the productive tasks (vocabulary, grammar, and phonological processing and moderate on the receptive tasks (vocabulary and grammar. L1 exposure correlated positively with the vocabulary size and phonological processing. Grammar scores were not related to the levels of L1 exposure, but were predicted by general cognitive abilities. L2 exposure negatively influenced productive grammar in L1, suggesting possible L2 transfer effects on L1 grammatical performance. Children’s narrative skills benefitted from exposure to two languages

  17. Nikkaji Dictionary: 3,5-ジブロモ-L-チロシン [MeCab user dictionary for science technology term[Archive

    Lifescience Database Archive (English)

    Full Text Available MeCab user dictionary for science technology term 3,5-ジブロモ-L-チロシン 名詞 一般 * * * * 3,5-ジブロモ-L-チロシン... ... Nikkaji J193.705G 200906036602581994 C CA06 UNKNOWN_2 3 , 5 - ジブロモ - L - チロシン

  18. Nikkaji Dictionary: 3,5-ジヨード-L-チロシン [MeCab user dictionary for science technology term[Archive

    Lifescience Database Archive (English)

    Full Text Available MeCab user dictionary for science technology term 3,5-ジヨード-L-チロシン 名詞 一般 * * * * 3,5-ジヨード-L-チロシン... ... Nikkaji J4.841K 200906024456017099 C CA06 UNKNOWN_2 3 , 5 - ジヨード - L - チロシン

  19. Bis[μ-2,5-bis(pyridin-2-yl-1,3,4-thiadiazole-κ4N2,N3:N4,N5]bis[(nitrato-κOsilver(I] tetrahydrate

    Directory of Open Access Journals (Sweden)

    Abdelhakim Laachir

    2013-06-01

    Full Text Available The self-assembly of an angular 2,5-bis(pyridin-2-yl-1,3,4-thiadiazole ligand (L with silver nitrate (AgNO3 produced a new dinuclear silver(I coordination complex, [Ag2(C12H8N4S2(NO32]·4H2O, which crystallizes with two Ag atoms bridged by two L ligands. The Ag atom is surrounded by four N atoms of L and by one O from the nitrate anion defining a distorted square pyramid. The atoms comprising the dication are nearly coplanar, with an r.m.s. deviation of 0.1997 Å. Molecules are linked by C—H...O and O—H...O hydrogen bonds through nitrate anions and water molecules, forming a two-dimensional porous network. The overall structure involves stacking of Ag complex layers along the b axis. The cohesion in the three-dimensional architecture is ensured by O...Ag interactions.

  20. Biallelic expression of Tssc4, Nap1l4, Phlda2 and Osbpl5 in adult ...

    Indian Academy of Sciences (India)

    2College of Life Science and Life Engineering, Science and Technology University of ... some assembly protein that belongs to the NAPs family of ... ing the cholesterol balance in the body. .... Similarly, our work also showed that Nap1l4 was.

  1. Asimmetrie L1/L2: una sfida nella didattica di “lingua e traduzione”.

    Directory of Open Access Journals (Sweden)

    Laura Salmon

    2004-12-01

    Full Text Available Asymmetries Between L1 and L2: a Challenge in the Teaching of "Language and Translation" Language teaching is deeply connected to the cognitive and brain sciences. The attribution of meaning of linguistic messages depends on the interaction between the communicant’s brain and the external world. The interaction is based on the cognitive and emotional experience shared by a L-community. Competence in L2 is acquired by the development of an internal data base of contextualised .units of living speech. (Lurija 1976. L2 teaching is particularly successful when the L2 units are inscribed in memory by means of the functionally equivalent L1 units. Selection of the L1/L2 correspondent units is due to the principle of markedness: each unit of L1 has a functional equivalent in one and only one unit of the L2. Because of the interlingual asymmetries (a set of Italian/Russian examples is given, functional equivalence differs from morphosyntactic and lexical equivalence. The competency in ascribing a degree of markedness to each linguistic unit needs the regular implicit acquisition of the L2 intonational and prosodic system; the processes of the metalinguistic conscious reflection are instead a .hindrance. to the procedural acting of a “living speaker”.

  2. A Prerequisite to L1 Homophone Effects in L2 Spoken-Word Recognition

    Science.gov (United States)

    Nakai, Satsuki; Lindsay, Shane; Ota, Mitsuhiko

    2015-01-01

    When both members of a phonemic contrast in L2 (second language) are perceptually mapped to a single phoneme in one's L1 (first language), L2 words containing a member of that contrast can spuriously activate L2 words in spoken-word recognition. For example, upon hearing cattle, Dutch speakers of English are reported to experience activation…

  3. Do L5 and s1 nerve root compressions produce radicular pain in a dermatomal pattern?

    Science.gov (United States)

    Taylor, Christopher S; Coxon, Andrew J; Watson, Paul C; Greenough, Charles G

    2013-05-20

    Observational case series. To compare the pattern of distribution of radicular pain with published dermatome charts. Dermatomal charts vary and previous studies have demonstrated significant individual subject variation. Patients with radiologically and surgically proven nerve root compression (NRC) caused by prolapsed intervertebral disc completed computerized diagrams of the distribution of pain and pins and needles. Ninety-eight patients had L5 compressions and 83 had S1 compressions. The distribution of pain and pins and needles did not correspond well with dermatomal patterns. Of those patients with L5 NRC, only 22 (22.4%) recorded any hits on the L5 dermatome on the front, and only 60 (61.2%) on the back with only 13 (13.3%) on both. Only 1 (1.0%) patient placed more than 50% of their hits within the L5 dermatome. Of those patients with S1 NRC, only 3 (3.6%) recorded any hits on the S1 dermatome on the front, and only 64 (77.1%) on the back with only 15 (18.1%) on both. No patients placed more than 50% of their hits within the S1 dermatome. Regarding pins and needles, 27 (29.7%) patients with L5 NRC recorded hits on the front alone, 27 (29.7%) on the back alone, and 14 (15.4%) on both. Nineteen (20.9%) recorded more than 50% of hits within the L5 dermatome. Three (3.6%) patients with S1 NRC recorded hits on the front alone, 44 (53.0%) on the back alone, and 18 (21.7%) on both. Twelve (14.5%) recorded more than 50% of hits within the S1 dermatome. Patient report is an unreliable method of identifying the anatomical source of pain or paresthesia caused by nerve root compression. 4.

  4. CdBr2 complexes of 1,2-bis-[2-(5-H/methyl/chloro/nitro)-1H-benzimidazolyl]-1,2-ethanediols

    International Nuclear Information System (INIS)

    Aydin Tavman

    2005-01-01

    The complexes of 1,2-bis-[2-(5-H/methyl/chloro/nitro)-1H-benzimidazolyl]-1,2-ethanediols with CdBr 2 were synthesized and characterized by elemental analysis, molar conductivity, IR and NMR spectra. The ligands act as a bidentate only through both oxygen atoms of hydroxyl groups in complexes with ratio M:L=1:1 [ru

  5. Manganese(I-Based CORMs with 5-Substituted 3-(2-PyridylPyrazole Ligands

    Directory of Open Access Journals (Sweden)

    Ralf Mede

    2017-01-01

    Full Text Available The reaction of [(OC5MnBr] with substituted 3-(2-pyridylpyrazoles 2-PyPzRH (1a-l in methanol or diethyl ether yields the yellow to orange manganese(I complexes [(OC3Mn(Br(2-PyPzRH] (2a-l, the substituents R being phenyl (a, 1-naphthyl (b, 2-anthracenyl (c, 1-pyrenyl (d, 4-bromophenyl (e, 3-bromophenyl (f, duryl (g, 2-pyridyl (h, 2-furanyl (i, 2-thienyl (j, ferrocenyl (k, and 1-adamantyl (l. The carbonyl ligands are arranged facially, leading to three chemically different CO ligands due to different trans-positioned Lewis donors. The diversity of the substituent R demonstrates that this photoCORM backbone can easily be varied with a negligible influence on the central (OC3MnBr fragment, because the structural parameters and the spectroscopic data of this unit are very similar for all these derivatives. Even the ferrocenyl complex 2k shows a redox potential for the ferrocenyl subunit which is identical to the value of the free 5-ferrocenyl-3-(2-pyridylpyrazole (1k. The ease of variation of the starting 5-substituted 3-(2-pyridylpyrazoles offers a modular system to attach diverse substituents at the periphery of the photoCORM complex.

  6. L1 and L2 Strategy Use in Reading Comprehension of Chinese EFL Readers

    Science.gov (United States)

    Tsai, Yea-Ru; Ernst, Cheryl; Talley, Paul C.

    2010-01-01

    This study revealed the relationship between L1 (Mandarin Chinese) and L2 (English) strategy use in L2 reading comprehension by focusing on the correlation of L1 reading ability, L2 proficiency and employed reading strategies. The participants, 222 undergraduates learning English as a foreign language (EFL), were classified into skilled and…

  7. [Effect of acetic acid, furfural and 5-hydroxymethylfurfural on production of 2,3-butanediol by Klebsiella oxytoca].

    Science.gov (United States)

    Wu, Jing; Cheng, Keke; Li, Wenying; Feng, Jie; Zhang, Jian'an

    2013-03-01

    To get the tolerability and consumption of Klebsiella oxytoca on major inhibitors in lignocelluloses hydrolysate, we studied the effect of acetic acid, furfural and 5-hydroxymethylfurfural on production of 2,3-butanediol by Klebsiella oxytoca. The metabolites of furfural and 5-hydroxymethylfurfural were measured. The results show that when acetic acid, furfural and 5-hydroxymethylfurfural was individually added, tolerance threshold for Klebsiella oxytoca was 30 g/L, 4 g/L and 5 g/L, respectively. Acetic acid was likely used as substrate to produce 2,3-butanediol. The yield of 2,3-butanediol increased when acetic acid concentration was lower than 30 g/L. In the fermentation, more than 70% 5-hydroxymethylfurfural was converted to 2,5-furandimethanol. All furfural and the rest of 5-hydroxymethylfurfural were metabolized by Klebsiella oxytoca. It showed that in the detoxification process of 2,3-butanediol production using lignocelluloses hydrolysate, furfural should be given priority to remove and a certain concentration of acetic acid is not need to removal.

  8. Cross-linking of L5 protein to 5 S RNA in rat liver 60-S subunits by ultraviolet irradiation

    International Nuclear Information System (INIS)

    Terao, K.; Uchiumi, T.; Ogata, K.

    1980-01-01

    After rat liver 60-S ribosomal subunits were irradiated with ultraviolet light at 254 nm, they were treated with EDTA and then subjected to sucrose density-gradient centrifugation to isolate 5 S RNA-protein complex. When 5 S RNA-protein was analyzed by SDS-acrylamide gel electrophoresis which dissociated noncovalent 5 S RNA-protein, two protein bands were observed. The one showed a slower mobility than the protein band (L5) of 5 S RNA-protein from non-irradiated 60 S subunit and the other showed the same mobility as L5 protein. Since the former band was shown to be specific to ultraviolet-irradiation, it was considered as cross-linked 5 S RNA-protein. After the two protein bands were iodinated with 125 I, labeled protein was extracted and treated with RNAase. Thereafter, it was analyzed by two-dimensional acrylamide gel electrophoresis, followed by autoradiography. The results indicate that the protein component of cross-linked 5 S RNA-protein is L5 protein (ribosomal protein); these proteins are designated according to the proposed uniform nomenclature. (Auth.)

  9. Synthesis, structure, luminescent, and magnetic properties of carbonato-bridged Zn(II)2Ln(III)2 complexes [(μ4-CO3)2{Zn(II)L(n)Ln(III)(NO3)}2] (Ln(III) = Gd(III), Tb(III), Dy(III); L(1) = N,N'-bis(3-methoxy-2-oxybenzylidene)-1,3-propanediaminato, L(2) = N,N'-bis(3-ethoxy-2-oxybenzylidene)-1,3-propanediaminato).

    Science.gov (United States)

    Ehama, Kiyomi; Ohmichi, Yusuke; Sakamoto, Soichiro; Fujinami, Takeshi; Matsumoto, Naohide; Mochida, Naotaka; Ishida, Takayuki; Sunatsuki, Yukinari; Tsuchimoto, Masanobu; Re, Nazzareno

    2013-11-04

    Carbonato-bridged Zn(II)2Ln(III)2 complexes [(μ4-CO3)2{Zn(II)L(n)Ln(III)(NO3)}2]·solvent were synthesized through atmospheric CO2 fixation reaction of [Zn(II)L(n)(H2O)2]·xH2O, Ln(III)(NO3)3·6H2O, and triethylamine, where Ln(III) = Gd(III), Tb(III), Dy(III); L(1) = N,N'-bis(3-methoxy-2-oxybenzylidene)-1,3-propanediaminato, L(2) = N,N'-bis(3-ethoxy-2-oxybenzylidene)-1,3-propanediaminato. Each Zn(II)2Ln(III)2 structure possessing an inversion center can be described as two di-μ-phenoxo-bridged {Zn(II)L(n)Ln(III)(NO3)} binuclear units bridged by two carbonato CO3(2-) ions. The Zn(II) ion has square pyramidal coordination geometry with N2O2 donor atoms of L(n) and one oxygen atom of a bridging carbonato ion at the axial site. Ln(III) ion is coordinated by nine oxygen atoms consisting of four from the deprotonated Schiff-base L(n), two from a chelating nitrate, and three from two carbonate groups. The temperature-dependent magnetic susceptibilities in the range 1.9-300 K, field-dependent magnetization from 0 to 5 T at 1.9 K, and alternating current magnetic susceptibilities under the direct current bias fields of 0 and 1000 Oe were measured. The magnetic properties of the Zn(II)2Ln(III)2 complexes are analyzed on the basis of the dicarbonato-bridged binuclear Ln(III)-Ln(III) structure, as the Zn(II) ion with d(10) electronic configuration is diamagnetic. ZnGd1 (L(1)) and ZnGd2 (L(2)) show a ferromagnetic Gd(III)-Gd(III) interaction with J(Gd-Gd) = +0.042 and +0.028 cm(-1), respectively, on the basis of the Hamiltonian H = -2J(Gd-Gd)ŜGd1·ŜGd2. The magnetic data of the Zn(II)2Ln(III)2 complexes (Ln(III) = Tb(III), Dy(III)) were analyzed by a spin Hamiltonian including the crystal field effect on the Ln(III) ions and the Ln(III)-Ln(III) magnetic interaction. The Stark splitting of the ground state was so evaluated, and the energy pattern indicates a strong easy axis (Ising type) anisotropy. Luminescence spectra of Zn(II)2Tb(III)2 complexes were observed, while those

  10. Modeling the Global Coronal Field with Simulated Synoptic Magnetograms from Earth and the Lagrange Points L3, L4, and L5

    Science.gov (United States)

    Petrie, Gordon; Pevtsov, Alexei; Schwarz, Andrew; DeRosa, Marc

    2018-06-01

    The solar photospheric magnetic flux distribution is key to structuring the global solar corona and heliosphere. Regular full-disk photospheric magnetogram data are therefore essential to our ability to model and forecast heliospheric phenomena such as space weather. However, our spatio-temporal coverage of the photospheric field is currently limited by our single vantage point at/near Earth. In particular, the polar fields play a leading role in structuring the large-scale corona and heliosphere, but each pole is unobservable for {>} 6 months per year. Here we model the possible effect of full-disk magnetogram data from the Lagrange points L4 and L5, each extending longitude coverage by 60°. Adding data also from the more distant point L3 extends the longitudinal coverage much further. The additional vantage points also improve the visibility of the globally influential polar fields. Using a flux-transport model for the solar photospheric field, we model full-disk observations from Earth/L1, L3, L4, and L5 over a solar cycle, construct synoptic maps using a novel weighting scheme adapted for merging magnetogram data from multiple viewpoints, and compute potential-field models for the global coronal field. Each additional viewpoint brings the maps and models into closer agreement with the reference field from the flux-transport simulation, with particular improvement at polar latitudes, the main source of the fast solar wind.

  11. Applications of the Buckeye GTA Corpus for L2 teaching and research

    DEFF Research Database (Denmark)

    Hardman, Jocelyn; McCullough, Elizabeth

    The Buckeye GTA Corpus contains 9,664 L1 and L2 sentence productions by 89 talkers (27 American English, 19 Hindi, 23 Mandarin, & 20 Korean). A total of 5,696 sentences were read in English, with each talker contributing 64 sentences. Hindi, Mandarin, and Korean talkers also read 64 sentences each...

  12. Distortion of He(2l2l') Fano lineshapes by strong post-collision interaction in H+-He collisions

    International Nuclear Information System (INIS)

    Moretto-Capelle, P.; Benhenni, M.; Bordenave-Montesquieu, D.; Bordenave-Montesquieu, A.

    1996-01-01

    The three-body post-collisional interaction (PCI) between the scattered proton, recoil target ion and emitted electron has been investigated by electron spectrometry near the 2l2l' helium resonances, in the 20-100 keV energy range (V p 0.9-2 au). Particular attention has been paid to the PCI deformations of the Fano lineshapes when V-vector'' p ≅ V-vector e (2l2l'). Their angle and collision velocity dependences have been studied for the first time experimentally. A large variety of lineshapes have been observed, all of them successfully described by a single formula. At the lowest proton velocities the rescattering effect (also called Coulomb two-path scattering) is seen. (Author)

  13. L(2, 1-Labelings of Some Families of Oriented Planar Graphs

    Directory of Open Access Journals (Sweden)

    Sen Sagnik

    2014-02-01

    Full Text Available In this paper we determine, or give lower and upper bounds on, the 2-dipath and oriented L(2, 1-span of the family of planar graphs, planar graphs with girth 5, 11, 16, partial k-trees, outerplanar graphs and cacti.

  14. PSA Nadir of <0.5 ng/mL Following Brachytherapy for Early-Stage Prostate Adenocarcinoma is Associated With Freedom From Prostate-Specific Antigen Failure

    Energy Technology Data Exchange (ETDEWEB)

    Ko, Eric C. [Department of Radiation Oncology, Mount Sinai Medical Center, New York, NY (United States); Stone, Nelson N. [Department of Radiation Oncology, Mount Sinai Medical Center, New York, NY (United States); Department of Urology, Mount Sinai Medical Center, New York, NY (United States); Stock, Richard G., E-mail: Richard.Stock@mountsinai.org [Department of Radiation Oncology, Mount Sinai Medical Center, New York, NY (United States)

    2012-06-01

    Purpose: Because limited information exists regarding whether the rate or magnitude of PSA decline following brachytherapy predicts long-term clinical outcomes, we evaluated whether achieving a prostate-specific antigen (PSA) nadir (nPSA) <0.5 ng/mL following brachytherapy is associated with decreased PSA failure and/or distant metastasis. Methods and Materials: We retrospectively analyzed our database of early-stage prostate adenocarcinoma patients who underwent brachytherapy, excluding those receiving androgen-deprivation therapy and those with <2 years follow-up. Median and mean pretreatment PSA were 6 ng/mL and 7.16 ng/mL, respectively. By clinical stage, 775 were low risk ({<=}T2a), 126 were intermediate risk (T2b), and 20 were high risk (>T2b). By Gleason score, 840 were low risk ({<=}6), 71 were intermediate risk (7), and 10 were high risk (>7). Patients were treated with brachytherapy only (I-125, n = 779, or Pd-103, n = 47), or brachytherapy + external-beam radiation therapy (n = 95). Median follow-up was 6.3 years. We noted whether nPSA <0.5 ng/mL was achieved and the time to achieve this nadir and tested for associations with pretreatment risk factors. We also determined whether this PSA endpoint was associated with decreased PSA failure or distant metastasis. Results: Absence of high-risk factors in clinical stage ({<=}T2b), Gleason score ({<=}7), and pretreatment PSA ({<=}20 ng/mL) was significantly associated with achieving nPSA <0.5 ng/mL. By Kaplan-Meier analysis, patients achieving nPSA <0.5 ng/mL had significantly higher long-term freedom from biochemical failure (FFBF) than nonresponders (5-year FFBF: 95.2 {+-} 0.8% vs. 71.5 {+-} 6.7%; p < 0.0005). Among responders, those who achieved nPSA <0.5 ng/mL in {<=}5 years had higher FFBF than those requiring >5 years (5-year FFBF: 96.7 {+-} 0.7% vs. 80.8 {+-} 4.6%; p < 0.0005). On multivariate analysis, patients who achieved nPSA <0.5 ng/mL in {<=}5 years had significantly higher FFBF than other

  15. Direct and rapid effects of 3,5-diiodo-L-thyronine (T2).

    Science.gov (United States)

    Moreno, Maria; Giacco, Antonia; Di Munno, Celia; Goglia, Fernando

    2017-12-15

    A growing number of researchers are focusing their attention on the possibility that thyroid hormone metabolites, particularly 3,5-diiodothyronine (T2), may actively regulate energy metabolism at the cellular, rather than the nuclear, level. Due to their biochemical features, mitochondria have been the focus of research on the thermogenic effects of thyroid hormones. Indeed, mitochondrial activities have been shown to be regulated both directly and indirectly by T2-specific pathways. Herein, we describe the effects of T2 on energy metabolism. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. Supramolecular assemblies in [Cu(L-Arg){sub 2}(H{sub 2}O)]C{sub 2}O{sub 4}·6H{sub 2}O complex – Structural, spectroscopic, magnetic and thermal behavior

    Energy Technology Data Exchange (ETDEWEB)

    Wojciechowska, Agnieszka, E-mail: agnieszka.wojciechowska@pwr.edu.pl [Faculty of Chemistry, Wroclaw University of Technology, Wybrzeze Wyspiańskiego 27, 50-370, Wrocław (Poland); Kochel, Andrzej [Faculty of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50-383, Wrocław (Poland); Duczmal, Marek [Faculty of Chemistry, Wroclaw University of Technology, Wybrzeze Wyspiańskiego 27, 50-370, Wrocław (Poland)

    2016-10-01

    The reaction of L-arginine and oxalate ions with copper(II) salts yields a new complex with formula of [Cu(L-Arg){sub 2}(H{sub 2}O)]·C{sub 2}O{sub 4}·6H{sub 2}O (1) (where L-Arg = L-arginine). Single crystals of 1 were synthesized by crystallization from aqueous solution. The complex properties were characterized by X-ray diffraction, spectroscopy (FT-IR, FT-Raman, NIR-Vis-UV and EPR) as well as thermal and magnetic methods. The square pyramidal (SP) geometry around Cu(II) ions in [Cu(L-Arg){sub 2}(H{sub 2}O)]{sup 2+} cation complex is formed by two cis-chelated L-arginine zwitterions and a water molecule coordinated in the apex of square pyramid. The trigonality distortion of SP geometry is relatively small, τ = 0.0087. The solid state EPR spectrum showed broad hyperfine splitting with g{sub ⊥} = 2.061 at 77 K. The copper centres distanced at 7.558(5) Å are joined in a single zig-zag structure via a chain based on the combination of Cu−O(5)−H(29)⋯O(2)−C1−O1−Cu hydrogen bonds along the b axis (d (O2⋯O5) = 2.812 Å). Taking into account the structural features, the magnetic susceptibility data were best-fitted, giving the exchange parameter J = −0.16 cm{sup −1}. Complex 1 is thermally stable up to 66 °C, where it was observed to lose the crystallization water molecules with an 11.7% mass loss (calc. 11.5%). - Highlights: • Crystal and molecular structure of [Cu(L-Arg){sub 2}(H{sub 2}O)]C{sub 2}O{sub 4}·6H{sub 2}O crystals have been studied. • The magnetic interactions of Cu(II) centres are assisted by the formation of single zig-zag chain. • Role of oxalate ions in completed relatively small square pyramid distortion is described. • The cis-fashioned L-arginine created the stronger ligand field than trans-configuration.

  17. Spondylolysis and End Plate Arthrosis at L5-S1: A Cadaveric Study.

    Science.gov (United States)

    McCunniff, Peter T; Yoo, Hojun; Yu, Charles; Bajwa, Navkirat S; Toy, Jason O; Ahn, Uri M; Ahn, Nicholas

    2017-01-01

    This study examined the effect of bilateral and unilateral L5 pars defects on the degree of disk degeneration at the L5-S1 level in cadaveric specimens. An observational study was performed of 690 cadaveric specimens selected at random. These specimens represent individuals who died between 1893 and 1938. The study included 558 male and 132 female cadavers. Of the 120 specimens with L5 spondylolysis, 95 cases were bilateral and 25 were unilateral. The remaining 544 specimens were used as the control cohort. Degenerative disk disease was measured by the classification of Eubanks et al. According to this classification, degenerative disk disease was graded from no arthrosis (grade 0) to complete ankylosis (grade IV). Linear regression analysis corrected for age, sex, and race showed that subjects with bilateral spondylolysis at L5 had a statistically significant increase in the amount of disk degeneration (P=.02) compared with those with unilateral lesions. Student's t tests showed significant differences (Pspondylolysis above what would be predicted in the normal control population. A positive correlation was found between the number of pars defects at L5 and the degree of disk degeneration at L5-S1. These results support the idea that individuals with spondylolysis at these levels may be at increased risk for development of low back pain and reduced quality of life. [Orthopedics. 2017; 40(1):e59-e64.]. Copyright 2016, SLACK Incorporated.

  18. Urinary 2,5-hexanedione in workers exposed to n-hexane: influence of the sample treatment

    Directory of Open Access Journals (Sweden)

    Daniela Magalhães Nolasco

    2007-08-01

    Full Text Available The aim of the present study was to determine 2,5-hexanedione (2,5-HD, a metabolite of n-hexane, by gas chromatography/flame ionization detection in 31 workers exposed to n-hexane after two types of sample pretreatment, i.e., with (total 2,5-HD and without (free 2,5-HD acid hydrolysis. The mean urinary 2,5-HD was 0.52 mg/L (free and 2.88 mg/L (total, this difference being significant (Student t-test, p < 0.05. The differences in the results according to the sample treatment support the need to modify the current Brazilian legislation, which proposes the analysis of 2,5-HD without indicating whether it is the free or total metabolite.

  19. A L2HGDH initiator methionine codon mutation in a Yorkshire terrier with L-2-hydroxyglutaric aciduria

    Directory of Open Access Journals (Sweden)

    Farias Fabiana HG

    2012-07-01

    Full Text Available Abstract Background L-2-hydroxyglutaric aciduria is a metabolic repair deficiency characterized by elevated levels of L-2-hydroxyglutaric acid in urine, blood and cerebrospinal fluid. Neurological signs associated with the disease in humans and dogs include seizures, ataxia and dementia. Case presentation Here we describe an 8 month old Yorkshire terrier that presented with episodes of hyperactivity and aggressive behavior. Between episodes, the dog’s behavior and neurologic examinations were normal. A T2 weighted MRI of the brain showed diffuse grey matter hyperintensity and a urine metabolite screen showed elevated 2-hydroxyglutaric acid. We sequenced all 10 exons and intron-exon borders of L2HGDH from the affected dog and identified a homozygous A to G transition in the initiator methionine codon. The first inframe methionine is at p.M183 which is past the mitochondrial targeting domain of the protein. Initiation of translation at p.M183 would encode an N-terminal truncated protein unlikely to be functional. Conclusions We have identified a mutation in the initiation codon of L2HGDH that is likely to result in a non-functional gene. The Yorkshire terrier could serve as an animal model to understand the pathogenesis of L-2-hydroxyglutaric aciduria and to evaluate potential therapies.

  20. Protection to glycolysis by a combination of 5-hydroxy-L-tryptophan and 2-aminoethylisothiuronium bromide hydrobromide in lethally irradiated rats

    International Nuclear Information System (INIS)

    Basu, S.K.; Srinivasan, M.N.; Chuttani, K.; George, S.

    1992-01-01

    Rate of glycolysis in vivo at different time intervals following 8 Gy[LDsub(100(30)] whole body gamma radiation (WBGR) was evaluated by estimating liver glycogen, blood sugar, serum lactic dehydrogenase (LDH) and lactic acid concentration in adult male Sprague Dawley rats. Within 1 hr of radiation exposure, a significant fall in liver glycogen was observed in rats fed food and water ad libitum. The glycogen content increased after 24 hr and had returned to control level on 7th day after radiation exposure. Blood sugar, serum LDH and blood lactate levels increased significantly as compared to non irradiated controls. Pretreatment with 5-hydroxy-L-tryptophan (5-HTP;100 mg/kg) + 2-aminoethylisothiuronium bromide hydrobromide (AET;20 mg/kg)ip 30 min before 8 Gy WBGR, modified these values and restored them to normal level on 7th day post-irradiation. (author). 24 refs

  1. Hallucinogenic 5-HT2AR agonists LSD and DOI enhance dopamine D2R protomer recognition and signaling of D2-5-HT2A heteroreceptor complexes.

    Science.gov (United States)

    Borroto-Escuela, Dasiel O; Romero-Fernandez, Wilber; Narvaez, Manuel; Oflijan, Julia; Agnati, Luigi F; Fuxe, Kjell

    2014-01-03

    Dopamine D2LR-serotonin 5-HT2AR heteromers were demonstrated in HEK293 cells after cotransfection of the two receptors and shown to have bidirectional receptor-receptor interactions. In the current study the existence of D2L-5-HT2A heteroreceptor complexes was demonstrated also in discrete regions of the ventral and dorsal striatum with in situ proximity ligation assays (PLA). The hallucinogenic 5-HT2AR agonists LSD and DOI but not the standard 5-HT2AR agonist TCB2 and 5-HT significantly increased the density of D2like antagonist (3)H-raclopride binding sites and significantly reduced the pKiH values of the high affinity D2R agonist binding sites in (3)H-raclopride/DA competition experiments. Similar results were obtained in HEK293 cells and in ventral striatum. The effects of the hallucinogenic 5-HT2AR agonists on D2R density and affinity were blocked by the 5-HT2A antagonist ketanserin. In a forskolin-induced CRE-luciferase reporter gene assay using cotransfected but not D2R singly transfected HEK293 cells DOI and LSD but not TCB2 significantly enhanced the D2LR agonist quinpirole induced inhibition of CRE-luciferase activity. Haloperidol blocked the effects of both quinpirole alone and the enhancing actions of DOI and LSD while ketanserin only blocked the enhancing actions of DOI and LSD. The mechanism for the allosteric enhancement of the D2R protomer recognition and signalling observed is likely mediated by a biased agonist action of the hallucinogenic 5-HT2AR agonists at the orthosteric site of the 5-HT2AR protomer. This mechanism may contribute to the psychotic actions of LSD and DOI and the D2-5-HT2A heteroreceptor complex may thus be a target for the psychotic actions of hallunicogenic 5-HT2A agonists. Copyright © 2013 Elsevier Inc. All rights reserved.

  2. The Impact of Different Support Vectors on GOSAT-2 CAI-2 L2 Cloud Discrimination

    Directory of Open Access Journals (Sweden)

    Yu Oishi

    2017-11-01

    Full Text Available Greenhouse gases Observing SATellite-2 (GOSAT-2 will be launched in fiscal year 2018. GOSAT-2 will be equipped with two sensors: the Thermal and Near-infrared Sensor for Carbon Observation (TANSO-Fourier Transform Spectrometer 2 (FTS-2 and the TANSO-Cloud and Aerosol Imager 2 (CAI-2. CAI-2 is a push-broom imaging sensor that has forward- and backward-looking bands to observe the optical properties of aerosols and clouds and to monitor the status of urban air pollution and transboundary air pollution over oceans, such as PM2.5 (particles less than 2.5 micrometers in diameter. CAI-2 has important applications for cloud discrimination in each direction. The Cloud and Aerosol Unbiased Decision Intellectual Algorithm (CLAUDIA1, which applies sequential threshold tests to features is used for GOSAT CAI L2 cloud flag processing. If CLAUDIA1 is used with CAI-2, it is necessary to optimize the thresholds in accordance with CAI-2. However, CLAUDIA3 with support vector machines (SVM, a supervised pattern recognition method, was developed, and then we applied CLAUDIA3 for GOSAT-2 CAI-2 L2 cloud discrimination processing. Thus, CLAUDIA3 can automatically find the optimized boundary between clear and cloudy areas. Improvements in CLAUDIA3 using CAI (CLAUDIA3-CAI continue to be made. In this study, we examined the impact of various support vectors (SV on GOSAT-2 CAI-2 L2 cloud discrimination by analyzing (1 the impact of the choice of different time periods for the training data and (2 the impact of different generation procedures for SV on the cloud discrimination efficiency. To generate SV for CLAUDIA3-CAI from MODIS data, there are two times at which features are extracted, corresponding to CAI bands. One procedure is equivalent to generating SV using CAI data. Another procedure generates SV for MODIS cloud discrimination at the beginning, and then extracts decision function, thresholds, and SV corresponding to CAI bands. Our results indicated the following

  3. Roles of Human CYP2A6 and Monkey CYP2A24 and 2A26 Cytochrome P450 Enzymes in the Oxidation of 2,5,2',5'-Tetrachlorobiphenyl.

    Science.gov (United States)

    Shimada, Tsutomu; Kakimoto, Kensaku; Takenaka, Shigeo; Koga, Nobuyuki; Uehara, Shotaro; Murayama, Norie; Yamazaki, Hiroshi; Kim, Donghak; Guengerich, F Peter; Komori, Masayuki

    2016-12-01

    2,5,2',5'-Tetrachlorobiphenyl (TCB) induced type I binding spectra with cytochrome P450 (P450) 2A6 and 2A13, with K s values of 9.4 and 0.51 µM, respectively. However, CYP2A6 oxidized 2,5,2',5'-TCB to form 4-hydroxylated products at a much higher rate (∼1.0 minute -1 ) than CYP2A13 (∼0.02 minute -1 ) based on analysis by liquid chromatography-tandem mass spectrometry. Formation of 4-hydroxy-2,5,2',5'-TCB by CYP2A6 was greater than that of 3-hydroxy-2,5,2',5'-TCB and three other hydroxylated products. Several human P450 enzymes, including CYP1A1, 1A2, 1B1, 2B6, 2D6, 2E1, 2C9, and 3A4, did not show any detectable activities in oxidizing 2,5,2',5'-TCB. Cynomolgus monkey CYP2A24, which shows 95% amino acid identity to human CYP2A6, catalyzed 4-hydroxylation of 2,5,2',5'-TCB at a higher rate (∼0.3 minute -1 ) than CYP2A26 (93% identity to CYP2A6, ∼0.13 minute -1 ) and CYP2A23 (94% identity to CYP2A13, ∼0.008 minute -1 ). None of these human and monkey CYP2A enzymes were catalytically active in oxidizing other TCB congeners, such as 2,4,3',4'-, 3,4,3',4'-, and 3,5,3',5'-TCB. Molecular docking analysis suggested that there are different orientations of interaction of 2,5,2',5'-TCB with the active sites (over the heme) of human and monkey CYP2A enzymes, and that ligand interaction energies (U values) of bound protein-ligand complexes show structural relationships of interaction of TCBs and other ligands with active sites of CYP2A enzymes. Catalytic differences in human and monkey CYP2A enzymes in the oxidation of 2,5,2',5'-TCB are suggested to be due to amino acid changes at substrate recognition sites, i.e., V110L, I209S, I300F, V365M, S369G, and R372H, based on the comparison of primary sequences. Copyright © 2016 by The American Society for Pharmacology and Experimental Therapeutics.

  4. Effectiveness of L2 spinal nerve infiltration for selective discogenic low back pain patients

    International Nuclear Information System (INIS)

    Ohtori, Seiji; Nakamura, Shinichiro; Koshi, Takana

    2010-01-01

    It has been reported that rat L5/6 lumbar discs are innervated mainly by L2 dorsal root ganglion neurons. We previously reported that L2 spinal nerve infiltration was effective for discogenic low back pain (DLBP) patients, although the diagnosis was based only on the results of physical examination, plain films, and magnetic resonance imaging (MRI). The purpose of the current study was to evaluate L2 spinal nerve block for DLBP patients retrospectively based on MRI findings and surgical results. A total of 62 patients with only LBP and no accompanying radicular pain were investigated. Patients had only one level of disc degeneration on MRI. When pain was provoked during discography, we performed surgery at the next stage (40 patients). In all, 22 patients were excluded owing to negative discography results. Of the 40 patients, we evaluated 25 strictly selected patients suffering from DLBP. DLBP was diagnosed when the patient experienced pain relief at least 2 years after anterior lumbar interbody fusion. Fifteen patients who did not show pain relief after surgery were used for the non-DLBP group. L2 spinal nerve infiltration using 1.5 ml of lidocaine was performed in all 40 patients before surgery. The visual analogue scale (VAS) score after L2 spinal nerve infiltration was recorded, and an association of L2 spinal nerve infiltration and DLBP was explored. Low back pain scores assessed using the VAS score, the Japanese Orthopedic Association score, and the Oswestry Disability Index score in the two groups were not significantly different. L2 spinal nerve infiltration was effective for 27 patients but not effective for 13 patients; the VAS score after 15 min and 2 h improved in the DLBP group compared with that of the non-DLBP group (P<0.05).