Sample records for lineweaver-burk plots showed
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Diffusional falsification of kinetic constants on Lineweaver-Burk plots.
Ghim, Y S; Chang, H N
1983-11-07
The effect of mass transfer resistances on the Lineweaver-Burk plots in immobilized enzyme systems has been investigated numerically and with analytical approximate solutions. While Hamilton, Gardner & Colton (1974) studied the effect of internal diffusion resistances in planar geometry, our study was extended to the combined effect of internal and external diffusion in cylindrical and spherical geometries as well. The variation of Lineweaver-Burk plots with respect to the geometries was minimized by modifying the Thiele modulus and the Biot number with the shape factor. Especially for a small Biot number all the three Lineweaver-Burk plots fell on a single line. As was discussed by Hamilton et al. (1974), the curvature of the line for large external diffusion resistances was small enough to be assumed linear, which was confirmed from the two approximate solutions for large and small substrate concentrations. Two methods for obtaining intrinsic kinetic constants were proposed: First, we obtained both maximum reaction rate and Michaelis constant by fitting experimental data to a straight line where external diffusion resistance was relatively large, and second, we obtained Michaelis constant from apparent Michaelis constant from the figure in case we knew maximum reaction rate a priori.
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Sajid-Ur-Rehman; Saeed, Aamer; Saddique, Gufran; Ali Channar, Pervaiz; Ali Larik, Fayaz; Abbas, Qamar; Hassan, Mubashir; Raza, Hussain; Fattah, Tanzeela Abdul; Seo, Sung-Yum
2018-06-02
To seek the new medicinal potential of sulfadiazine drug, the free amino group of sulfadiazine was exploited to obtain acyl/aryl thioureas using simple and straightforward protocol. Acyl/aryl thioureas are well recognized bioactive pharmacophore containing moieties. A new series (4a-4j) of sulfadiazine derived acyl/aryl thioureas was synthesized and characterized through spectroscopic and elemental analysis. The synthesized derivatives 4a-4j were subjected to calf intestinal alkaline phosphatase (CIAP) activity. The derivative 4a-4j showed better inhibition potential compared to standard monopotassium phosphate (MKP). The compound 4c exhibited higher potential in the series with IC 50 0.251 ± 0.012 µM (standard KH 2 PO 4 4.317 ± 0.201 µM). Lineweaver-Burk plots revealed that most potent derivative 4c inhibition CIAP via mixed type pathway. Pharmacological investigations showed that synthesized compounds 4a-4j obey Lipinsk's rule. ADMET parameters evaluation predicted that these molecule show significant lead like properties with minimum possible toxicity and can serve as templates in drug designing. The synthetic compounds show none mutagenic and irritant behavior. Molecular docking analysis showed that compound 4c interacts with Asp273, His317 and Arg166 amino acid residues. Copyright © 2018. Published by Elsevier Ltd.
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Albumin's Influence on Carprofen Enantiomers-Hymecromone Interaction.
Tang, Mingjie; Guo, Yanjie; Gao, Youshui; Tang, Chao; Dang, Xiaoqian; Zhou, Zubin; Sun, Yuqiang; Wang, Kunzheng
2016-03-01
Hymecromone is an important coumarin drug, and carprofen is one of the most important nonsteroidal antiinflammatory drugs (NSAIDs). The present study aims to determine the influence of bovine serum albumin (BSA) on the carprofen-hymecromone interaction. The inhibition of carprofen enantiomers on the UDP-glucuronosyltransferase (UGT) 2B7-catalyzed glucuronidation of hymecromone was investigated in the UGTs incubation system with and without BSA. The inhibition capability of increased by 20% (P carprofen after the addition of 0.5% BSA in the incubation mixture. In contrast, no significant difference was observed for the inhibition of (S)-carprofen on UGT2B7 activity in the absence or presence of 0.5% BSA in the incubation system. The Lineweaver-Burk plot showed that the intersection point was located in the vertical axis, indicating the competitive inhibition of (R)-carprofen on UGT2B7 in the incubation system with BSA, which is consistent with the inhibition kinetic type of (R)-carprofen on UGT2B7 in the incubation system without BSA. Furthermore, the second plot using the slopes from the Lineweaver-Burk versus the concentrations of (R)-carprofen showed that the fitting equation was y=39.997x+50. Using this equation, the inhibition kinetic parameter was calculated to be 1.3 μM. For (S)-carprofen, the intersection point was located in the horizontal axis in the Lineweaver-Burk plot for the incubation system with BSA, indicating the noncompetitive inhibition of (S)-carprofen on the activity of UGT2B7. The fitting plot of the second plot was y=24.6x+180, and the inhibition kinetic parameter was 7.3 μM. In conclusion, the present study gives a short summary of BSA's influence on the carprofen enantiomers-hymecromone interaction, which will guide the clinical application of carprofen and hymecromone. © 2015 Wiley Periodicals, Inc.
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Determination Of Enzyme Kinetic Parameters on Sago Starch Hydrolysis By Linearized Graphical Methods
International Nuclear Information System (INIS)
Lai, L.W.; Teo, C.L.; Suzana Wahidin; Mohamad Suffian Mohamad Annuar
2014-01-01
Amyloglucosidase (E.C. 3.2.1.3) from Aspergillus niger was used to hydrolyze the sago (Metro xylon sagu) starch into reducing sugars. The experiment was conducted at constant temperature, 55 degree Celsius; pH, 4.5 and enzyme amount, 0.2 U/ ml, respectively. In this investigation, the substrate concentration was varied ranging from 1.0 - 7.0 g/ L. The obtained data were then fixed into linearized plots namely Lineweaver-Burk and Langmuir models to calculate enzyme kinetic parameters, K m and V max . Both of the K m and V max (mM, mol/min) values from each plot were: Lineweaver-Burk (26.53, 3.31) and Langmuir (13.52, 2.35). Among the linearized models, K m and V max values acquired from Langmuir plot was chosen. (author)
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Teaching Burke: Kenneth Burke and the Rhetoric of Ascent.
Quinn, Arthur
1995-01-01
Advises that in teaching Kenneth Burke it is important that students understand that his strength is not what Richard Rorty calls systematic reasoning but rather edification. Shows how Burke, like Thomas Carlyle, is prophet or sage who sketches out a Romantic world view. (TB)
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Shrestha, Riya; Kim, Ju-Hyun; Nam, Wongshik; Lee, Hye Suk; Lee, Jae-Mok; Lee, Sangkyu
2018-04-01
Fisetin is a flavonol compound commonly found in edible vegetables and fruits. It has anti-tumor, antioxidant, and anti-inflammatory effects. Geraldol, the O-methyl metabolite of fisetin in mice, is reported to suppress endothelial cell migration and proliferation. Although the in vivo and in vitro effects of fisetin and its metabolites are frequently reported, studies on herb-drug interactions have not yet been performed. This study was designed to investigate the inhibitory effect of fisetin and geraldol on eight isoforms of human cytochrome P450 (CYP) by using cocktail assay and LC-MS/MS analysis. The selective inhibition of CYP2C8-catalyzed paclitaxel hydroxylation by fisetin and geraldol were confirmed in pooled human liver microsomes (HLMs). In addition, an IC 50 shift assay under different pre-incubation conditions confirmed that fisetin and geraldol shows a reversible concentration-dependent, but not mechanism-based, inhibition of CYP2C8. Moreover, Michaelis-Menten, Lineweaver-burk plots, Dixon and Eadie-Hofstee showed a non-competitive inhibition mode with an equilibrium dissociation constant of 4.1 μM for fisetin and 11.5 μM for geraldol, determined from secondary plot of the Lineweaver-Burk plot. In conclusion, our results indicate that fisetin showed selective reversible and non-competitive inhibition of CYP2C8 more than its main metabolite, geraldol, in HLMs. Copyright © 2018 The Japanese Society for the Study of Xenobiotics. Published by Elsevier Ltd. All rights reserved.
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A robust methodology for kinetic model parameter estimation for biocatalytic reactions
DEFF Research Database (Denmark)
Al-Haque, Naweed; Andrade Santacoloma, Paloma de Gracia; Lima Afonso Neto, Watson
2012-01-01
lead to globally optimized parameter values. In this article, a robust methodology to estimate parameters for biocatalytic reaction kinetic expressions is proposed. The methodology determines the parameters in a systematic manner by exploiting the best features of several of the current approaches...... parameters, which are strongly correlated with each other. State-of-the-art methodologies such as nonlinear regression (using progress curves) or graphical analysis (using initial rate data, for example, the Lineweaver-Burke plot, Hanes plot or Dixon plot) often incorporate errors in the estimates and rarely...
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Japan Report, Science and Technology.
1987-05-06
Chibata, KOTEIKA KOSO [IMMOBILIZED ENZYME], Kodansha Publishing Co., 1975. 2. K. Mosback, "Methods in Enzymology ," IMMOBILIZED ENZYME, Vol 44...continuously in a high molecular solute mixture, an invertase immobilized membrane with separa- tion functions is needed which enables the high...the Lineweaver-Burk plot of hydrolysis reactions of a sucrose solution when passing through an invertase immobilized membrane 22 which is prepared
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Study on the interaction of bovine serum albumin and fleroxacin by fluorescence method
International Nuclear Information System (INIS)
Nie Lihua; Zhao Huichun; Wang Xuebin; Wang Xu
2001-01-01
A fluorescence method is used to study the fluorescence quenching of bovine serum albumin by its interaction with fleroxacin. The interaction association constants of bovine serum albumin and fleroxacin are determined from a double reciprocal Lineweaver-Burk plot. According to the Foester dipole-dipole energy transfer, the distance to be measured between the fleroxacin and tryptophane is 4.37 nm. From thermodynamical coordination it can be judged that the binding power between fleroxacin and bovine serum albumin is static electric power
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Guillemette, G; Favreau, I; Lamontagne, S; Boulay, G
1990-04-25
Inositol 1,4,5-trisphosphate (InsP3) is an important second messenger generated from the hydrolysis of phosphatidylinositol 4,5-bisphosphate by phospholipase C in response to Ca2(+)-mobilizing stimuli. InsP3 interacts with specific intracellular receptors and triggers the release of sequestered Ca2+ from an intracellular store. We have looked at the influence of 2,3-diphosphoglycerate on the action and metabolism of InsP3 in the bovine adrenal cortex. 2,3-Diphosphoglycerate blocked InsP3 binding to adrenal cortex microsomes with a half-maximal efficiency of 0.5 mM. Scatchard analyses revealed that 2,3-diphosphoglycerate did not change the maximal capacity of the microsomes, but decreased their binding affinity for InsP3. The Ca2(+)-releasing activity of InsP3 on the same microsomal preparation was monitored with the fluorescent indicator, Fura-2. 2,3-Diphosphoglycerate blocked this activity with a half-maximal efficiency of 2 mM. The effect of 2,3-diphosphoglycerate could be overcome by supramaximal doses of InsP3, indicating a competitive inhibitory effect. The activity of InsP3 phosphatase from bovine adrenal cortex microsomes was also studied. 2,3-Diphosphoglycerate inhibited the activity of the phosphatase with a half-maximal efficiency of 0.3 mM. Lineweaver-Burke plots revealed that this effect was competitive. Finally, 2,3-diphosphoglycerate was also able to inhibit the activity of a partially purified preparation of InsP3 kinase from bovine adrenal cortex cytosol. The half-maximal dose was around 10 mM and the Lineweaver-Burke plot showed that the inhibition was competitive. These results show that 2,3-diphosphoglycerate can be considered as a structural analog of InsP3. Its inhibitory effects, however, are not selective enough to use it as an InsP3 protective agent in Ca2(+)-mobilization studies.
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Tyrosinase Inhibition Type of Isolated Compounds Obtained from Pachyrhizus erosus
Directory of Open Access Journals (Sweden)
Endang Lukitaningsih
2013-12-01
Full Text Available In Indonesia, Bengkoang (Phacyrhizus erosus have been used as one of cosmetics especially as sun screening and skin whitening materials. Six active compounds in Bengkoang with antioxidant and skin whitening activities have been isolated, namely daidzein, daidzin, genistin, (8,9-furanyl-pterocarpan-3-ol, 4-(2-(furane-2-ylethyl-2-methyl-2,5-dihydro-furane-3-carbaldehyde and 2-butoxy-2,5-bis(hydroxymethyl-tetrahydrofurane-3,4-diol. According to literatures, the type of their tyrosinase inhibitory activity has not yet reported. The determination of whitening activity of each compound was evaluated by the evaluation of Lineweaver-Burk plot. The result showed that five compounds had competitive inhibitory activity and 8,9-furanyl-pterocarpan-3-ol showed a non-competitive inhibition.
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Kinetics Investigation on Mushroom Tyrosinase Inhibition of Proso Millet
Directory of Open Access Journals (Sweden)
Wen-Ying Huang
2018-01-01
Full Text Available Proso millet (Panicum miliaceum is rich in nutritive components and is widely used as a human food, feed and forage for animals, and fuel. This study investigated the effect of a proso millet extract on the inhibition of tyrosinase, a key enzyme in melanogenesis. High performance liquid chromatography analysis indicated that the proso millet extract contained phenolic tyrosinase inhibitors, such as syringic acid, p-coumaric acid, and ferulic acid. The extract had an IC50 for inhibition of tyrosinase activity of 14.02 mg/mL. A Lineweaver-Burk double reciprocal plot showed that the proso millet extract functioned as a mixed competitive and noncompetitive inhibitor. Proso millet has potential as a tyrosinase inhibitor that may have applications in the cosmetics industry.
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Zhang, Ting-Jian; Li, Song-Ye; Yuan, Wei-Yan; Wu, Qing-Xia; Wang, Lin; Yang, Su; Sun, Qi; Meng, Fan-Hao
2017-02-15
A series of (1H-1,2,3-triazol-4-yl)methoxybenzaldehyde derivatives containing an anthraquinone moiety were synthesized and identified as novel xanthine oxidase inhibitors. Among them, the most promising compounds 1h and 1k were obtained with IC 50 values of 0.6μM and 0.8μM, respectively, which were more than 10-fold potent compared with allopurinol. The Lineweaver-Burk plot revealed that compound 1h acted as a mixed-type xanthine oxidase inhibitor. SAR analysis showed that the benzaldehyde moiety played a more important role than the anthraquinone moiety for inhibition potency. The basis of significant inhibition of xanthine oxidase by 1h was rationalized by molecular modeling studies. Copyright © 2017 Elsevier Ltd. All rights reserved.
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Obituary: Edward W. Burke, Jr. (1924-2011)
Bloomer, Raymond, Jr.
2011-12-01
Dr. Edward W. Burke Jr. passed away on June 15, 2011, after suffering a heart attack. Dr. Burke devoted his professional life to the research and teaching of physics and astronomy at King College in Bristol, Tennessee. Edward W. Burke, Jr., was born in Macon, Georgia, on September 16, 1924. He was a Navy veteran, having been commissioned as an ensign in 1944. He served in the Pacific near the end of World War II. He proceeded to complete his undergraduate degree in mathematics from Presbyterian College in 1947 and pursued the M.S. and Ph.D. in physics (1949 and 1954, respectively) at the University of Wisconsin. Under the direction of Professor Julian Mack, his thesis was titled "Isotope Shift in the Spectra of Boron." Although he did research in atomic spectra in the early part of his career, his interest in astronomy and variable stars in particular were his primary interests during his long academic career. Dr. Burke began his illustrious career at King College in 1949. He initiated the astronomy program there in 1950, included constructing a 12.5 inch Newtonian telescope, homemade as was most everything in those days. Many of his students learned about photometry at the Burke Observatory on the college campus. Burke was known for his trips to the Kitt Peak and Lowell observatories accompanied by undergraduate students on his trips, all of which were made by automobile which he preferred over flying. His initial interest in Ap stars later broadened into variable and especially eclipsing binary stars. His motivation was maintained by his desire to have his students experience basic research and to spark their interest in advanced degrees. Numerous students achieved advanced science and medical degrees because of Burke's encouragement and mentoring. In 1959, Dr. Burke was awarded a Fulbright professorship and traveled to Chile where he taught physics for a year in the Engineering School at the University of Chile in Santiago. He worked to establish a physics
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Luo, Wen; Zhao, Yong-mei; Tian, Run-guo; Su, Ya-bin; Hong, Chen
2013-11-01
A novel series of bis-nicotine derivatives (3a-3i) were designed, synthesized and evaluated as bivalent anti-Alzheimer's disease agents. The pharmacological results indicated that compounds 3e-3i inhibited both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in the micromolar range (IC50, 2.28-117.86 micromol x L(-1) for AChE and 1.67-125 micromol x L(-1) for BChE), which was at the same potency as rivastigmine. A Lineweaver-Burk plot and molecular modeling study showed that these derivatives targeted both the catalytic active site (CAS) and the peripheral anionic site (PAS) of AChE. Besides, these compounds could significantly inhibit the self-induced Abeta aggregation with inhibition activity (11.85%-62.14%) at the concentration of 20 micromol x L(-1).
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Fincher-Burke excitations in single-Q chromium
Böni, P; Stadler, C; Roessli, B; Shirane, G; Werner, S A
2002-01-01
The low-energy excitations of incommensurate antiferromagnetic Cr have been investigated by means of high-resolution, inelastic neutron scattering with unpolarized, cold neutrons within an energy range E<9 meV. In agreement with previous measurements we observe Fincher-Burke excitations in the transverse spin density wave phase that appear between the unresolved spin-wave peaks at the incommensurate positions Q sup+-=(1+- delta,0,0). In contrast to the previous measurements, our high-resolution data shows that the Fincher-Burke modes do not follow a linear dispersion. Therefore, they have nothing in common with the acoustic phonon branch. The major part of the scattering is concentrated in the range 4 meV
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International Nuclear Information System (INIS)
Deocaris, Custer C.; Cruz, Angela C; Dela Rosa, Alumanda M.; De Guzman, Roche Ray C.; Abilo, Rhea Mae L.; Gutierrez, Ana Kamila O.; Deocaris, Chester C.
2001-01-01
κ carrageenan and its radiolytic products are both shown to strongly inhibit pepsin activity based on our in vitro peptic ulcer model. Generated Lineweaver-Burke (LB) plots resemble the trend for competitive inhibition except that the changes in the slopes are too large rendering Km to approach a negative value. Our observations corroborate with the published dual behavior of carrageenan in inhibiting peptic ulcers, that is, by exerting simultaneously both enzyme (E) competitive inhibition and substrate (S) occlusion. Irradiated-carrageenan does not display anti-protozoan activity against Tetrahymena and pathogenic Entamoeba histolytica, and anti-tumor potential based on Artemia salina (brine shrimp) nauplus tests and the MTT assay with human MCF7 breast carcinoma cell line. (Author)
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Directory of Open Access Journals (Sweden)
Charles O. Nwamba
2012-01-01
Full Text Available Inactivation of purified β-Galactosidase was done with GdnHCl in the absence and presence of varying [galactose] at 50°C and at pH 4.5. Lineweaver-Burk plots of initial velocity data, in the presence and absence of guanidine hydrochloride (GdnHCl and galactose, were used to determine the relevant and max values, with p-nitrophenyl β-D-galactopyranoside (pNPG as substrate, S. Plots of ln([]∞−[] against time in the presence of GdnHCl yielded the inactivation rate constant, A. Plots of A versus [S] at different galactose concentrations were straight lines that became increasingly less steep as the [galactose] increased, showing that A was dependent on [S]. Slopes and intercepts of the 1/[]∞ versus 1/[] yielded +0 and ′+0, the microscopic rate constants for the free enzyme and the enzyme-substrate complex, respectively. Plots of +0 and ′+0 versus [galactose] showed that galactose protected the free enzyme as well as the enzyme-substrate complex (only at the lowest and highest [galactose] against GdnHCl inactivation. In the absence of galactose, GdnHCl exhibited some degree of non-competitive inhibition. In the presence of GdnHCl, galactose exhibited competitive inhibition at the lower [galactose] of 5 mM which changed to non-competitive as the [galactose] increased. The implications of our findings are further discussed.
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BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.
Directory of Open Access Journals (Sweden)
Jolene R Bowers
2010-11-01
Full Text Available A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.
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Teaching Burke Using Advertisements.
Larson, Charles U.
Kenneth Burke's concepts of identification, the five terms of dramatism, and strategic uses of ambiguity can be successfully taught to undergraduates if appropriate and familiar examples are used. Print and electronic advertising offer the instructor an up-to-date, familiar, and abundant source of classroom examples. Market segmentation models…
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2012-08-24
... Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington... of human remains under the control of the Thomas Burke Memorial Washington State Museum (Burke Museum...
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International Nuclear Information System (INIS)
Gheibi, N.; Sarreshtehdari, M.; Saboury, A. A.
2011-01-01
Tyrosinase is a widespread enzyme with great promising capabilities. The Lineweaver-Burk plots of the catecholase reactions showed that the kinetics of mushroom tyrosinase (MT), activated by Co"2"+ and Zn"2"+ at different pHs (6, 7, 8 and 9) obeyed the non-essential activation mode. The binding of metal ions to the enzyme increases the maximum velocity of the enzyme due to an increase in the enzyme catalytic constant (k_c_a_t). From the kinetic analysis, dissociation constants of the activator from the enzyme-metal ion complex (K_a) were obtained as 5 x 10"4 M"-"1 and 8.33 x 10"3 M"-"1 for Co"2"+ and Zn"2"+ at pH 9 and 6 respectively. The structural analysis of MT through circular dichroism (CD) and intensive fluorescence spectra revealed that the conformational stability of the enzyme in these pHs reaches its maximum value in the presence of each of the two metal ions
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A BOD monitoring disposable reactor with alginate-entrapped bacteria.
Villalobos, Patricio; Acevedo, Cristian A; Albornoz, Fernando; Sánchez, Elizabeth; Valdés, Erika; Galindo, Raúl; Young, Manuel E
2010-10-01
Biochemical oxygen demand (BOD) is a measure of the amount of dissolved oxygen that is required for the biochemical oxidation of the organic compounds in 5 days. New biosensor-based methods have been conducted for a faster determination of BOD. In this study, a mathematical model to evaluate the feasibility of using a BOD sensor, based on disposable alginate-entrapped bacteria, for monitoring BOD in situ was applied. The model considers the influences of alginate bead size and bacterial concentration. The disposable biosensor can be adapted according to specific requirements depending on the organic load contained in the wastewater. Using Klein and Washausen parameter in a Lineweaver-Burk plot, the glucose diffusivity was calculated in 6.4 × 10(-10) (m2/s) for beads of 1 mm in diameter and slight diffusion restrictions were observed (n = 0.85). Experimental results showed a correlation (p < 0.05) between the respirometric peak and the standard BOD test. The biosensor response was representative of BOD.
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Amylolytic hydrolysis of native starch granules affected by granule surface area.
Kim, J C; Kong, B W; Kim, M J; Lee, S H
2008-11-01
Initial stage of hydrolysis of native starch granules with various amylolytic enzymes, alpha-amylase from Bacillus subtilis, glucoamylase I (GA-I) and II (GA-II) from Aspergillus niger, and beta-amylase from sweet potato showed that the reaction was apparently affected by a specific surface area of the starch granules. The ratios of the reciprocal of initial velocity of each amylolytic hydrolysis for native potato and maize starch to that for rice with the amylolytic enzymes were nearly equivalent to the ratio of surface area per mass of the 2 starch granules to that of rice, that is, 6.94 and 2.25, respectively. Thus, the reciprocal of initial velocity of each enzymatic hydrolysis as expressed in a Lineweaver-Burk plot was a linear function of the reciprocal of surface area for each starch granule. As a result, it is concluded that amylolytic hydrolysis of native starch granules is governed by the specific surface area, not by the mass concentration, of each granule.
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Selected Enzyme Inhibitory Effects of Euphorbia characias Extracts
Directory of Open Access Journals (Sweden)
Antonella Fais
2018-01-01
Full Text Available Extracts of aerial part of Euphorbia characias were examined to check potential inhibitors for three selected enzymes involved in several metabolic disorders. Water and ethanol extracts from leaves and flowers showed in vitro inhibitory activity toward α-amylase, α-glucosidase, and xanthine oxidase. IC50 values were calculated for all the extracts and the ethanolic extracts were found to exert the best effect. In particular, for the α-glucosidase activity, the extracts resulted to be 100-fold more active than the standard inhibitor. The inhibition mode was investigated by Lineweaver-Burk plot analysis. E. characias extracts display different inhibition behaviors toward the three enzymes acting as uncompetitive, noncompetitive, and mixed-type inhibitors. Moreover, ethanolic extracts of E. characias showed no cytotoxic activity and exhibited antioxidant capacity in a cellular model. The LC-DAD metabolic profile was also performed and it showed that leaves and flowers extracts contain high levels of quercetin derivatives. The results suggest that E. characias could be a promising source of natural inhibitors of the enzymes involved in carbohydrate uptake disorders and oxidative stress.
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Aldose Reductase Inhibitory Activity of Compounds from Zea mays L.
Kim, Tae Hyeon; Kim, Jin Kyu; Kang, Young-Hee; Lee, Jae-Yong; Kang, Il Jun; Lim, Soon Sung
2013-01-01
Aldose reductase (AR) inhibitors have a considerable therapeutic potential against diabetes complications and do not increase the risk of hypoglycemia. Through bioassay-guided fractionation of an EtOH extract of the kernel from purple corn (Zea mays L.), 7 nonanthocyanin phenolic compounds (compound 1–7) and 5 anthocyanins (compound 8–12) were isolated. These compounds were investigated by rat lens aldose reductase (RLAR) inhibitory assays. Kinetic analyses of recombinant human aldose reductase (rhAR) were performed, and intracellular galactitol levels were measured. Hirsutrin, one of 12 isolated compounds, showed the most potent RLAR inhibitory activity (IC50, 4.78 μM). In the kinetic analyses using Lineweaver-Burk plots of 1/velocity and 1/substrate concentration, hirsutrin showed competitive inhibition against rhAR. Furthermore, hirsutrin inhibited galactitol formation in rat lens and erythrocytes sample incubated with a high concentration of galactose; this finding indicates that hirsutrin may effectively prevent osmotic stress in hyperglycemia. Therefore, hirsutrin derived from Zea mays L. may be a potential therapeutic agent against diabetes complications. PMID:23586057
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The Inhibitory Effect of Prunella vulgaris L. on Aldose Reductase and Protein Glycation
Directory of Open Access Journals (Sweden)
Hong Mei Li
2012-01-01
Full Text Available To evaluate the aldose reductase (AR enzyme inhibitory ability of Prunella vulgaris L. extract, six compounds were isolated and tested for their effects. The components were subjected to in vitro bioassays to investigate their inhibitory assays using rat lens aldose reductase (rAR and human recombinant AR (rhAR. Among them, caffeic acid ethylene ester showed the potent inhibition, with the IC50 values of rAR and rhAR at 3.2±0.55 μM and 12.58±0.32 μM, respectively. In the kinetic analyses using Lineweaver-Burk plots of 1/velocity and 1/concentration of substrate, this compound showed noncompetitive inhibition against rhAR. Furthermore, it inhibited galactitol formation in a rat lens incubated with a high concentration of galactose. Also it has antioxidative as well as advanced glycation end products (AGEs inhibitory effects. As a result, this compound could be offered as a leading compound for further study as a new natural products drug for diabetic complications.
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2010-08-24
... DEPARTMENT OF THE INTERIOR National Park Service Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service... of the Thomas Burke Memorial Washington State Museum (Burke Museum), University of Washington...
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2010-06-28
... DEPARTMENT OF THE INTERIOR National Park Service Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service... of the Thomas Burke Memorial Washington State Museum (Burke Museum), University of Washington...
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2011-09-19
... DEPARTMENT OF THE INTERIOR National Park Service [2253-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum (Burke...
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2011-09-19
... DEPARTMENT OF THE INTERIOR National Park Service [2253-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum (Burke...
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2011-09-19
... DEPARTMENT OF THE INTERIOR National Park Service [2253-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum (Burke...
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2013-10-25
... inventory of human remains under the control of the Burke Museum. The human remains were removed from Island....R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of... Memorial Washington State Museum, University of Washington (Burke Museum), has completed an inventory of...
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2013-09-30
... completion of an inventory of human remains under the control of the Burke Museum, Seattle, WA. The human....R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of... Memorial Washington State Museum, University of Washington (Burke Museum), has completed an inventory of...
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2013-09-30
....S.C. 3003, of the completion of an inventory of human remains under the control of the Burke Museum....R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of... Washington (Burke Museum), and Central Washington University have completed an inventory of human remains, in...
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Allosteric behavior in the activation of transducin mediated by rhodopsin
International Nuclear Information System (INIS)
Wessling-Resnick, M.; Johnson, G.I.
1986-01-01
Transducin is a member of the family of regulatory GTP-binding proteins which provide a signal transduction mechanism for many cell surface receptors. These receptors act in a catalytic manner to displace GDP bound to the G protein in exchange for GTP during a process referred to as activation. The authors have studied the steady-state kinetics of the activation of transducin mediated by rhodopsin by employing the non-hydrolyzable GTP analog, [ 35 S]-GTPγS. The substrate-velocity curves display remarkable allosteric behavior with a Hill coefficient, n/sub H/ = 2. Lineweaver-Burke plots with respect to reciprocal [transducin] show curvilinearity indicative of positive cooperativity. However, a series of parallel lines are generated by plotting the linear transformation as [transducin] -2 . The double reciprocal plots with respect to [GTPγS] are a series of parallel lines. The initial rate analysis supports a double displacement catalytic mechanism for the molecular interactions between the photon receptor, G protein, and guanine nucleotides. It remains to be determined whether the positive cooperative behavior the authors observe can be assigned to the interaction of multiple transducins with rhodopsin, the presence of an allosteric effector, or hysteresis in the receptor's activity. These unique observations also provide insight into the molecular interactions of members of the family of G protein-coupled receptors
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Triterpenes as uncompetitive inhibitors of α-glucosidase from flowers of Punica granatum L.
Salah El Dine, Riham; Ma, Qiong; Kandil, Zeinab A; El-Halawany, Ali M
2014-01-01
The α-glucosidase and maltase inhibitory effects of Punica granatum L. flowers (PGF) were investigated. The methanol extract (PGFMe), n-hexane extract (PGFH), chloroform extract (PGFC) and the remaining water fraction (PGFW) were assayed for their α-glucosidase and maltase inhibitory effects. PGFW showed potent α-glucosidase inhibition with IC₅₀ of 0.8 μg/mL followed by PGFMe (IC₅₀ of 4.0 μg/mL) then PGFC (IC₅₀ of 5.21 μg/mL) in comparison to acarbose (0.9 μM). Due to its selectivity towards α-glucosidase, PGFC was subjected to bioactivity-guided isolation of its main active constituents. Five known compounds (1-5) were identified as β-sitosterol (1), oleanolic acid (2), ursolic acid (3), p-coumaric acid (4) and apigenin (5). Ursolic and oleanolic acids showed potent α-glucosidase inhibition (IC₅₀ of 39.0 and 35.0 μM, respectively), while they did not show significant maltase inhibition. Kinetic study using the double Lineweaver-Burk plot revealed that ursolic acid uncompetitively inhibited α-glucosidase in comparison with acarbose as a competitive inhibitor.
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Anti-pepsin activity of silicon dioxide nanoparticles
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Hussein Kadhem Al-Hakeim
2016-09-01
Full Text Available SiO2NPs as an inhibitor of pepsin enzyme for treatment of gastro-esophageal reflux disease (GERD was used. Silicon dioxide nanoparticles (pepsin coated SiO2NPs are among the safest nanoparticles that can be used inside the human body. The activity of pepsin before and after the addition of certain amounts of the NPs to the reaction mixture was measured spectrophotometrically. Furthermore, these experiments were repeated at different temperatures, different weights of NPs, and different ionic strengths. The kinetic aparameters (Km & Vmax of the pepsin catalyzed reactions were calculated from the Lineweaver-Burke plots. The results showed that there is a significant reduction of pepsin activity by SiO2NPs (Vmax of free pepsin = 4.82 U and Vmax of the immobilizedpepsin = 2.90 U. The results also indicated that the presence of ionic strength causes remarkable reduction of pepsin activity. It can be concluded the best condition for inhibition of pepsin activity is by using a combination of SiO2NPs and high concentration NaCl at 37 °C.
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Directory of Open Access Journals (Sweden)
Р. В. Фафула
2017-12-01
Full Text Available Background: It is known that Na+,K+-ATP-ase plays important role in physiology of spermatozoa including their motility. Na+,K+-ATP-ase is one of the targets for reactive oxygen species. Hyperproduction of reactive oxygen species can damage sperm cells and it is considered to be as one of the mechanisms of male infertility. Objectives: To evaluate the H2O2 effect on the main kinetic parameters of ATP hydrolysis by ouabain-sensitive Na+,K+-ATPase of spermatozoa of fertile (normozoospermia and infertility men (asthenozoospermia. Materials and methods: Na+, K+-ATP-ase activity was determined spectrophotometrically by production of Pi. Concentration dependencies ware linearized in Lineweaver-Burk plot. Results: Effective inhibitory effect of H2O2 on ouabain-sensitive Na+,K+-ATP-ase activity of sperm cells of fertile and infertile men was demonstrated. The effects of H2O2 on the main kinetic parameters of the ATP hydrolysis with the involvement of Na+, K+-ATP-ase was studied. In the whole range of studied concentrations of ATP the Na+, K+-ATP-ase activity of spermatozoa of fertile and infertile men was reduced in the presence of H2O2 in the incubation medium. However, the optimal activity of the Na+, K+-ATP-ase activity of sperm cells in both normozoospermic and asthenozoospermic men was observed in the presence of 5 mM ATP in the incubation medium. By linearization of concentration curves in Lineweaver-Burk plot the main kinetic parameters of Na+, K+-activated, Mg2+-dependent ATP hydrolysis in the sperm cells of fertile and infertile men were determined. Under the effect of H2O2, the affinity constant of enzyme to ATP in normozoospermic and asthenozoospermic men increases several times. The initial maximum rate of ATP hydrolysis was significantly reduced only in the spermatozoa of fertile men with normozoospermia. Conclusions: Under conditions of H2O2-induced oxidative stress the inhibition of ouabain-sensitive Na+,K+-ATP-ase activity in sperm cells
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Taukoorah, Urmeela; Mahomoodally, M. Fawzi
2016-01-01
Aloe vera gel (AVG) is traditionally used in the management of diabetes, obesity, and infectious diseases. The present study aimed to investigate the inhibitory potential of AVG against α-amylase, α-glucosidase, and pancreatic lipase activity in vitro. Enzyme kinetic studies using Michaelis-Menten (K m) and Lineweaver-Burk equations were used to establish the type of inhibition. The antioxidant capacity of AVG was evaluated for its ferric reducing power, 2-diphenyl-2-picrylhydrazyl hydrate scavenging ability, nitric oxide scavenging power, and xanthine oxidase inhibitory activity. The glucose entrapment ability, antimicrobial activity, and total phenolic, flavonoid, tannin, and anthocyanin content were also determined. AVG showed a significantly higher percentage inhibition (85.56 ± 0.91) of pancreatic lipase compared to Orlistat. AVG was found to increase the Michaelis-Menten constant and decreased the maximal velocity (V max) of lipase, indicating mixed inhibition. AVG considerably inhibits glucose movement across dialysis tubes and was comparable to Arabic gum. AVG was ineffective against the tested microorganisms. Total phenolic and flavonoid contents were 66.06 ± 1.14 (GAE)/mg and 60.95 ± 0.97 (RE)/mg, respectively. AVG also showed interesting antioxidant properties. The biological activity observed in this study tends to validate some of the traditional claims of AVG as a functional food. PMID:26880905
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Materiality, Symbolicity, and the Rhetoric of Order: "Dialectical Biologism" as Motive in Burke.
Engnell, Richard A.
1998-01-01
Considers how the work of Kenneth Burke has recently been critiqued for its lack of attention to the role of non-symbolic motivation in rhetoric. Describes Burke's contributions as a "dialectical biologism" that sets forth a system of five symbolic/material dialectics that undergird all rhetorical appeal. Suggests that the most effective…
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Directory of Open Access Journals (Sweden)
Xueran Geng
2015-06-01
Full Text Available An 86-kDa homodimeric angiotensin I-converting enzyme (ACE inhibitory protein designated as LTP was isolated from fruit bodies of the mushroom Leucopaxillus tricolor. The isolation procedure involved ultrafiltration through a membrane with a molecular weight cutoff of 10-kDa, ion exchange chromatography on Q-Sepharose, and finally fast protein liquid chromatography-gel filtration on Superdex 75. LTP exhibited an IC50 value of 1.64 mg∙mL−1 for its ACE inhibitory activity. The unique N-terminal amino acid sequence of LTP was disclosed by Edman degradation to be DGPTMHRQAVADFKQ. In addition, seven internal sequences of LTP were elucidated by liquid chromatography-tandem mass spectrometry (LC-MS/MS analysis. Results of the Lineweaver-Burk plot suggested that LTP competitively inhibited ACE. Both LTP and the water extract of L. tricolor exhibited a clear antihypertensive effect on spontaneously hypertensive rats.
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Isolation and Characterization of an α-Glucosidase Inhibitor from Musa spp. (Baxijiao Flowers
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Zhanwu Sheng
2014-07-01
Full Text Available The use of α-glucosidase inhibitors is considered to be an effective strategy in the treatment of diabetes. Using a bioassay-guided fractionation technique, five Bacillus stearothermophilus α-glucosidase inhibitors were isolated from the flowers of Musa spp. (Baxijiao. Using NMR spectroscopy analysis they were identified as vanillic acid (1, ferulic acid (2, β-sitosterol (3, daucosterol (4 and 9-(4′-hydroxyphenyl-2-methoxyphenalen-1-one (5. The half maximal inhibitory concentration (IC50 values of compounds 1–5 were 2004.58, 1258.35, 283.67, 247.35 and 3.86 mg/L, respectively. Compared to a known α-glucosidase inhibitor (acarbose, IC50 = 999.31 mg/L, compounds 3, 4 and 5 showed a strong α-glucosidase inhibitory effect. A Lineweaver-Burk plot indicated that compound 5 is a mixed-competitive inhibitor, while compounds 3 and 4 are competitive inhibitors. The inhibition constants (Ki of compounds 3, 4 and 5 were 20.09, 2.34 and 4.40 mg/L, respectively. Taken together, these data show that the compounds 3, 4 and 5 are potent α-glucosidase inhibitors.
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Directory of Open Access Journals (Sweden)
Basiru Olaitan Ajiboye
2016-09-01
Full Text Available Objective: To investigate the in vitro antioxidant activity of ethanolic extract of Artocarpus heterophyllus (A. heterophyllus stem bark and its inhibitory effect on a-amylase and a-glucosidase. Methods: The A. heterophyllus stem bark was extracted using methanol and tested for antioxidative activity. Results: The results revealed that the ethanolic extract has polyphenolics and free radical scavenging compounds which were significantly higher (P < 0.05 than their respective standard, at concentration dependent manner. The ethanolic extract of A. heterophyllus stem bark was observed to show inhibitory activities on a-amylase and a-glucosidase with IC50 of (4.18 ± 0.01 and (3.53 ± 0.03 mg/mL, respectively. The Lineweaver-Burk plot revealed that ethanolic extract of A. heterophyllus stem bark exhibited non-competitive inhibition for a-amylase and uncompetitive inhibition for a-glucosidase activities. Also, gas chromatography–mass spectrometry showed the presence of different bioactive compounds in extract. Conclusions: Therefore, it can be inferred from this study that ethanolic extract of A. heterophyllus stem bark may be useful in the management of diabetes mellitus probably due to bioactive compounds observed in the extract.
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Directory of Open Access Journals (Sweden)
Yun Li
2016-06-01
Full Text Available Angiotensin I-converting enzyme (ACE inhibitory activity of razor clam hydrolysates produced using five proteases, namely, pepsin, trypsin, alcalase, flavourzyme and proteases from Actinomucor elegans T3 was investigated. Flavourzyme hydrolysate showed the highest level of degree of hydrolysis (DH (45.87% followed by A. elegans T3 proteases hydrolysate (37.84% and alcalase (30.55%. The A. elegans T3 proteases was observed to be more effective in generating small peptides with ACE-inhibitory activity. The 3 kDa membrane permeate of A. elegans T3 proteases hydrolysate showed the highest ACE-inhibitory activity with an IC50 of 0.79 mg/mL. After chromatographic separation by Sephadex G-15 gel filtration and reverse phase-high performance liquid chromatography, the potent fraction was subjected to MALDI/TOF-TOF MS/MS for identification. A novel ACE-inhibitory peptide (VQY was identified exhibiting an IC50 of 9.8 μM. The inhibitory kinetics investigation by Lineweaver-Burk plots demonstrated that the peptide acts as a competitive ACE inhibitor. The razor clam hydrolysate obtained by A. elegans T3 proteases could serve as a source of functional peptides with ACE-inhibitory activity for physiological benefits.
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2010-06-28
... DEPARTMENT OF THE INTERIOR National Park Service Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA; Correction AGENCY: National Park... human remains and associated funerary objects in the possession of the Thomas Burke Memorial Washington...
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2012-08-02
... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-10823; 2200-1100-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington...
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2013-02-19
... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-12080;2200-1100-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington...
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2013-07-30
... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-13406; PPWOCRADN0-PCU00RP14.R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke...
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2013-09-30
... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-13770; PPWOCRADN0-PCU00RP14.R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of..., Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum, University of...
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2013-07-24
... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-13407; PPWOCRADN0-PCU00RP14.R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke...
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[Enzymatic conversion of tetradecanol in heterogenous phase by yeast-alcohol dehydrogenase].
Rothe, U; Schöpp, W; Aurich, H
1976-01-01
Alcohol dehydrogenase from yeast converts long-chain primary alcohols not only in the dissolved state, but also at the surface of undissolved particles. Tetradecanol beads with a defined surface can be produced and employed as model substrate. The reaction rate was determined by the proton release accomplished in the reaction. The initial reaction rate depends on the enzyme concentration. The relation is nonlinear (vi = k-[e]0,4); the numerical value of the exponent (n = 0.4) argues in favour of a reaction occurring at the interface. The Lineweaver-Burk plots become linear if the substrate concentrations are based on the molar surface concentrations of the particles. The pH optimum for the reaction at the surface is displaced by 0.25 pH units towards the alkaline region (compared with ethanol as substrate). The activation energy of the reaction with tetradecanol beads as substrate is 30% lower than that for the ethanol oxydation.
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"More Than Lessons in How To Read": Burke, Freud, and the Resources of Symbolic Transformation.
Quandahl, Ellen
2001-01-01
Argues that Kenneth Burke used "The Interpretation of Dreams," as well as other works by Sigmund Freud, as a lesson on reading, taking over the central tropes of dreamwork and making them broadly dialectical rather than strictly psychoanalytic terms. Suggests that Freud's "tropology" of dreaming is crucial for reading Burke.…
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Directory of Open Access Journals (Sweden)
Prachyaporn Inthongkaew
Full Text Available ABSTRACT A methanol extract from the whole plant of Dendrobium formosum Roxb. ex Lindl., Orchidaceae, showed inhibitory potential against α-glucosidase and pancreatic lipase enzymes. Chromatographic separation of the extract resulted in the isolation of twelve phenolic compounds. The structures of these compounds were determined through analysis of NMR and HR-ESI-MS data. All of the isolates were evaluated for their α-glucosidase and pancreatic lipase inhibitory activities, as well as glucose uptake stimulatory effect. Among the isolates, 5-methoxy-7-hydroxy-9,10-dihydro-1,4-phenanthrenequinone (12 showed the highest α-glucosidase and pancreatic lipase inhibitory effects with an IC50 values of 126.88 ± 0.66 µM and 69.45 ± 10.14 µM, respectively. An enzyme kinetics study was conducted by the Lineweaver-Burk plot method. The kinetics studies revealed that compound 12 was a non-competitive inhibitor of α-glucosidase and pancreatic lipase enzymes. Moreover, lusianthridin at 1 and 10 µg/ml and moscatilin at 100 µg/ml showed glucose uptake stimulatory effect without toxicity on L6 myotubes. This study is the first report on the phytochemical constituents and anti-diabetic and anti-obesity activities of D. formosum.
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2011-09-19
... DEPARTMENT OF THE INTERIOR National Park Service [2253-665] Notice of Intent To Repatriate Cultural Items: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum...
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Inhibition of 12/15 lipoxygenase by curcumin and an extract from Curcuma longa L.
Bezáková, Lýdia; Košťálová, Daniela; Obložinský, Marek; Hoffman, Peter; Pekárová, Mária; Kollárová, Renáta; Holková, Ivana; Mošovská, Silvia; Sturdík, Ernest
2014-02-01
Curcumin (diferuloylmethane) is an orange-yellow secondary metabolic compound from the rhizome of turmeric (Curcuma longa L.), a spice often found in curry powder. It is one of the major curcuminoids of turmeric. For centuries, curcumin has been used in some medicinal preparations or as a food colouring agent. A variety of enzymes that are closely associated with inflammation and cancer were found to be modulated by curcumin. This paper summarized the results of the inhibitory effect of curcumin and a Curcuma longa L. ethanolic extract on lipoxygenase from the rat lung cytosolic fraction. The positional specificity determination of arachidonic acid dioxygenation by RP- and SP-HPLC methods showed that in a purified enzyme preparation from the rat lung cytosol the specific form of lipoxygenase (LOX) is present exhibiting 12/15-LOX dual specificity (with predominant 15-LOX activity). The inhibitory activity of curcumin and Curcuma longa extract on LOX from cytosolic fraction of rat lung was expressed in the percentage of inhibition and as IC50. Lineweaver-Burk plot analysis has indicated that curcumin is the competitive inhibitor of 12/15 LOX from the rat lung cytosolic fraction.
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Aldose reductase inhibitory compounds from Xanthium strumarium.
Yoon, Ha Na; Lee, Min Young; Kim, Jin-Kyu; Suh, Hong-Won; Lim, Soon Sung
2013-09-01
As part of our ongoing search for natural sources of therapeutic and preventive agents for diabetic complications, we evaluated the inhibitory effects of components of the fruit of Xanthium strumarium (X. strumarium) on aldose reductase (AR) and galactitol formation in rat lenses with high levels of glucose. To identify the bioactive components of X. strumarium, 7 caffeoylquinic acids and 3 phenolic compounds were isolated and their chemical structures were elucidated on the basis of spectroscopic evidence and comparison with published data. The abilities of 10 X. strumarium-derived components to counteract diabetic complications were investigated by means of inhibitory assays with rat lens AR (rAR) and recombinant human AR (rhAR). From the 10 isolated compounds, methyl-3,5-di-O-caffeoylquinate showed the most potent inhibition, with IC₅₀ values of 0.30 and 0.67 μM for rAR and rhAR, respectively. In the kinetic analyses using Lineweaver-Burk plots of 1/velocity and 1/substrate, methyl-3,5-di-O-caffeoylquinate showed competitive inhibition of rhAR. Furthermore, methyl-3,5-di-O-caffeoylquinate inhibited galactitol formation in the rat lens and in erythrocytes incubated with a high concentration of glucose, indicating that this compound may be effective in preventing diabetic complications.
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International Nuclear Information System (INIS)
Sugavara, S.
1979-01-01
A simple and reliable method is described for the quantitative evaluation of vascular permeability increase induced by vasoactive drugs with Evans blue labelled with iodine-125 or 131. By using this method the importance of α-amino group of bradykinin (Bk), kallidin (Kd) and methionyl-kallidin (Met-Kd) on the biological activity were studied after reacting the kinins with pyridoxal 5'-phosphate followed by reduction with sodium borohydride. Phosphopyridoxyl-kinins were formed leaving free the guanidino groups. Aminoacid analysis of phosphopyridoxyl-kinin showed that the efficiency of the reaction was extremely good in the blockage of α-amino groups [phosphopyridoxyl-bradikinin (PP-Bk) = 98,8%, phosphopyridoxyl-kallidin (PP-Kd) = 95,2%, phosphopyridoxyl-methionyl-kallidin (PP-Met-Kd) = 98,0%. Log dose-response curves were obtained for Bk, Kd, Met-Kd, acetyl-bradykinin (Ac-Bk), PP-Bk, PP-Kd and PP-Met-Kd and the relative potencies calculated through the Lineweaver-Burk plots. The relative potencies were: PP-Bk about 16% the activity of Bk, Ac-Bk about 31% the activity of Bk, PP-Kd about 17% the activity of Kd, PP-Met-Kd about 12% the activity of Met-Kd. The results show that the terminal α-amino group of kinins is important in the mechanisms of biological activity. (Author) [pt
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Debate on sublime in the end of 18th century: Burke, Kant, Schiller
Directory of Open Access Journals (Sweden)
Jeremić-Molnar Dragana
2009-01-01
Full Text Available In the article the authors are examining three positions within the 18th Century aesthetic discussion on the sublime - Edmund Burke's, Immanuel Kant's and Friedrich Schiller's. They are also trying to reconstruct the political backgrounds of each of this theoretical positions: old regime conservatism (Burke, republican liberalism (Schiller and romantic longing for the 'third way' (Kant. The most sophisticated and mature theory of sublime is found in Schiller's aesthetic works, especially in those following his disappointment in French Revolution, in which the relationship between sublime and paradoxes of historical violence is most thoroughly reflected.
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Schultz, Pamela D
2011-01-01
Law consists of a series of stories, narratives that embody the values and integrity of a culture. We define crimes, and label the individuals who commit them, along a continuum that moves from the merely unacceptable to the monstrous. One of the most heinous crimes in American society is considered to be child sexual abuse. The sexual abuser of children is firmly established in the public imagination as a modern-day bogeyman, and approaches to prevention and punishment reflect this artificially accelerated panic. Although public policies and correctional measures ostensibly are geared at preventing the crime, they reflect a fundamental misunderstanding as to the motivation of many offenders and the way in which child sexual abuse is perceived as a source of social anomie. This paper uses Kenneth Burke's interpretative framework to examine the social and legal perceptions and treatment of child sexual abusers. First discussed are Burke's conceptions of identity and orientation, which form the basis of human motivation and thus can illuminate the source of offenders' impulses. Next, Burke's "terms for order" are used to show the ways in which the dominant hierarchy compels us to scapegoat and sacrifice in the quest for social redemption. Copyright © 2011 Elsevier Ltd. All rights reserved.
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Tu, Maolin; Liu, Hanxiong; Zhang, Ruyi; Chen, Hui; Mao, Fengjiao; Cheng, Shuzhen; Lu, Weihong; Du, Ming
2018-04-25
Casein hydrolysates exert various biological activities, and the responsible functional peptides are being identified from them continuously. In this study, the tryptic casein hydrolysate was fractionated by an ultrafiltration membrane (3 kDa), and the peptides were identified by capillary electrophoresis-quadrupole-time-of-flight-tandem mass spectrometry. Meanwhile, in silico methods were used to analyze the toxicity, solubility, stability, and affinity between the peptides and angiotensin-I-converting enzyme (ACE). Finally, a new angiotensin-I-converting enzyme inhibitory (ACEI) peptide, EKVNELSK, derived from α s1 -casein (fragment 35-42) was screened. The half maximal inhibitory concentration value of the peptide is 5.998 mM, which was determined by a high-performance liquid chromatography method. The Lineweaver-Burk plot indicated that this peptide is a mixed-type inhibitor against ACE. Moreover, Discovery Studio 2017 R2 software was adopted to perform molecular docking to propose the potential mechanisms underlying the ACEI activity of the peptide. These results indicated that EKVNELSK is a new ACEI peptide identified from casein hydrolysate.
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Das, Bipasha; Bhattacharjee, Sangita; Bhattacharjee, Chiranjib
2013-09-01
In this study, ultrafiltration (UF) of pretreated casein whey was carried out in a cross-flow module fitted with 5 kDa molecular weight cut-off polyethersulfone membrane to recover whey proteins in the retentate and lactose in the permeate. Effects of processing conditions, like transmembrane pressure and pH on permeate flux and rejection were investigated and reported. The polarised layer resistance was found to increase with time during UF even in this high shear device. The lactose concentration in the permeate was measured using dinitro salicylic acid method. Enzymatic kinetic study for lactose hydrolysis was carried out at three different temperatures ranging from 30 to 50 °C using β-galactosidase enzyme. The glucose formed during lactose hydrolysis was analyzed using glucose oxidase-peroxidase method. Kinetics of enzymatic hydrolysis of lactose solution was found to follow Michaelis-Menten model and the model parameters were estimated by Lineweaver-Burk plot. The hydrolysis rate was found to be maximum (with Vmax = 5.5091 mmol/L/min) at 30 °C.
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Burke, Nietzsche, Lacan: Three Perspectives on the Rhetoric of Order.
Thomas, Douglas
1993-01-01
Examines the complex relationship between rhetoric and order in the works of Kenneth Burke, Friedrich Nietzsche, and Jacques Lacan. Argues for three differing, yet complementary, views of rhetoric and order, each having a corresponding epistemology and axiology. Concludes with an analysis of the construction of order in Thomas Hobbe's…
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Rhetorical distance and knowledge in Burke's blurring of identification and division
DEFF Research Database (Denmark)
Kampf, Constance
for knowledge creation (Nonaka et al. 2000). This synthesis of perspectives offers insight into the connection between rhetoric and academic knowledge communication processes. Burke, K. (1966). Language as Symbolic Action: Essays on Life, Literature and Method. Berkeley, CA: University of California Press...
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Yu, Xiao-Dan; Xie, Jian-Hui; Wang, Yong-Hong; Li, Yu-Cui; Mo, Zhi-Zhun; Zheng, Yi-Feng; Su, Ji-Yan; Liang, Ye-er; Liang, Jin-Zhi; Su, Zi-Ren; Huang, Ping
2015-01-01
The aim of this study is to evaluate the antibacterial activity and urease inhibitory effects of patchouli alcohol (PA), the bioactive ingredient isolated from Pogostemonis Herba, which has been widely used for the treatment of gastrointestinal disorders. The activities of PA against selected bacteria and fungi were determined by agar dilution method. It was demonstrated that PA exhibited selective antibacterial activity against Helicobacter pylori, without influencing the major normal gastrointestinal bacteria. Noticeably, the antibacterial activity of PA was superior to that of amoxicillin, with minimal inhibition concentration value of 78 µg/mL. On the other hand, PA inhibited ureases from H.pylori and jack bean in concentration-dependent fashion with IC50 values of 2.67 ± 0.79 mM and 2.99 ± 0.41 mM, respectively. Lineweaver-Burk plots indicated that the type of inhibition was non-competitive against H.pylori urease whereas uncompetitive against jack bean urease. Reactivation of PA-inactivated urease assay showed DL-dithiothreitol, the thiol reagent, synergistically inactivated urease with PA instead of enzymatic activity recovery. In conclusion, the selective H.pylori antibacterial activity along with urease inhibitory potential of PA could make it a possible drug candidate for the treatment of H.pylori infection. Copyright © 2014 John Wiley & Sons, Ltd.
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Oztürk, Lokman; Bülbül, Metin; Elmastas, Mahfuz; Ciftçi, Mehmet
2007-01-01
In this study, catalase (CAT: EC 1.11.1.6) was purified from parsley (Petroselinum hortense) leaves; analysis of the kinetic behavior and some properties of the enzyme were investigated. The purification consisted of three steps, including preparation of homogenate, ammonium sulfate fractionation, and fractionation by DEAE-Sephadex A50 ion exchange chromatography. The enzyme was obtained with a yield of 9.5% and had a specific activity of 1126 U (mg proteins)(-1). The overall purification was about 5.83-fold. A temperature of 4 degrees C was maintained during the purification process. Enzyme activity was spectrophotometrically measured at 240 nm. In order to control the purification of the enzyme, SDS-polyacrylamide gel electrophoresis was carried out in 4% and 10% acryl amide for stacking and running gel, respectively. SDS-polyacrylamide gel electrophoresis showed a single band for the enzyme. The molecular weight was found to be 183.29 kDa by Sephadex G-200 gel filtration chromatography. The stable pH, optimum pH, and ionic strength were determined for phosphate and Tris-HCl buffer systems. In addition, K(M) and V(max) values for H(2)O(2), at optimum pH and 25 degrees C, were determined by means of Lineweaver-Burk plots.
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Constituents of antibacterial extract of Caesalpinia paraguariensis Burk.
Woldemichael, Girma M; Singh, Maya P; Maiese, William M; Timmermann, Barbara N
2003-01-01
The Argentinean legume Caesalpinia paraguariensis Burk. (Fabaceae) was selected for further fractionation work based on the strong antimicrobial activity of its CH2Cl2-MeOH (1:1 v/v) extract against a host of clinically significant microorganisms, including antibiotic resistant strains. 1D and 2D NMR enabled the identification of the novel benzoxecin derivative caesalpinol along with the known compounds bilobetin, stigma-5-en-3-O-beta-6'-stearoylglucopyranoside, stigma-5-en-3-beta-6'-palmitoylglucopyranoside, stigma-5-en-3-beta-glucopyranoside, oleanolic acid, 3-O-(E)-hydroxycinnamoyl oleanolic acid, betulinic acid, 3-O-(E)-hydroxycinnamoyl betulinic acid, and lupeol from the active fractions. Oleanolic acid was found active against Bacillus subtilis and both methicillin-sensitive and -resistant Staphylococcus aureus with MICs of 8 (17.5 microM), 8 and 64 (140 microM) microg/ml, respectively. The rest of the compounds, however, did not show activity.
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Bergmann, Jeannine; Krewer, Carmen; Rieß, Katrin; Müller, Friedemann; Koenig, Eberhard; Jahn, Klaus
2014-07-01
To compare the classification of two clinical scales for assessing pusher behaviour in a cohort of stroke patients. Observational case-control study. Inpatient stroke rehabilitation unit. A sample of 23 patients with hemiparesis due to a unilateral stroke (1.6 ± 0.7 months post stroke). Immediately before and after three different interventions, the Scale for Contraversive Pushing and the Burke Lateropulsion Scale were applied in a standardized procedure. The diagnosis of pusher behaviour on the basis of the Scale for Contraversive Pushing and the Burke Lateropulsion Scale differed significantly (χ2 = 54.260, p Pushing (χ2 = 19.148, p Pushing, but pusher behaviour on the Burke Lateropulsion Scale. 64.5% (20 of 31) of them scored on the Burke Lateropulsion Scale on the standing and walking items only. The Burke Lateropulsion Scale is an appropriate alternative to the widely used Scale for Contraversive Pushing to follow-up patients with pusher behaviour (PB); it might be more sensitive to detect mild pusher behaviour in standing and walking. © The Author(s) 2014.
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DEFF Research Database (Denmark)
Hammer, Bjørk; Hansen, Lars Bruno; Nørskov, Jens Kehlet
1999-01-01
A simple formulation of a generalized gradient approximation for the exchange and correlation energy of electrons has been proposed by Perdew, Burke, and Ernzerhof (PBE) [Phys. Rev. Lett. 77, 3865 (1996)]. Subsequently Zhang and Yang [Phys. Rev. Lett. 80, 890 (1998)] have shown that a slight...... revision of the PBE functional systematically improves the atomization energies for a large database of small molecules. In the present work, we show that the Zhang and Yang functional (revPBE) also improves the chemisorption energetics of atoms and molecules on transition-metal surfaces. Our test systems...
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Energy conserving site design case study, Burke Center, Virginia. Final report
Energy Technology Data Exchange (ETDEWEB)
1979-12-01
The pattern of energy consumption within a modern suburban community, Burke Center, is examined. A variety of actions that could be incorporated to reduce its future energy demands is proposed. Recommendations have been limited to those that are technically feasible today, are reasonably cost-effective, and that should generate little or no market resistance. Findings are that: of the total energy consumed by the Burke Center Community, 57% will go to off-site transportation needs; energy conserving site planning generally coincides with cost-effective site planning; water-to-air heat pumps proved to be the most efficient and cost-effective method available today in the study area for reducing heating and cooling costs in buildings; certain public services such as the collection of solid waste, mail pick-up and delivery, and other routine activities can be made more energy efficient; and the use of available water saving devices in residential and commercial buildings can reduce a community's total utility energy consumption by as much as 20%. (MCW)
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Kinetics and thermodynamic studies of alpha amylase from bacillus licheniformis mutant
International Nuclear Information System (INIS)
Ikram-ul-Haq, M.; Javed, M.M.; Hameed, U.; Adnan, F.
2010-01-01
The present investigation deals with the purification and characterization of enzyme a'-amylase from a mutant strain of Bacillus licheniformis EMS-6. A laboratory scale stirred fermentor of 7.5 L capacity was used for the enzyme production under optimal conditions. The enzyme was purified up to homogeneity level by Ammonium sulphate and ion-exchange chromatography using a fast protein liquid chromatography (FPLC) system. The specific activity of the enzyme increased 4-5 times while the yield was found to be 40.4%. The purification fold by RESOURCE-S was recorded to be 3.58. The molecular weight was found to be 55 KDa. In the present research work, the Vmax (2778 U/mg/min) and Km (8.3mg/ml) of a'-amylase were derived from the Lineweaver Burke plot. Thermodynamic parameters for soluble starch hydrolysis, Ea, AH, AS and AG of a'-amylase from B. licheniformis EMS-6 were found to be 25.14 KJ/mol, 22.53 KJ/mole, -110.95 J/mole/K and 36968 J/mole, respectively. The enzyme was stable over a pH range of 4.5-9.0 and gave pH optimum of 7.0. The pKa1 and pKa2 of ionizable groups of active site controlling Vmax, determined by Dixon plot, were 6.0 and 7.5, respectively. (author)
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Monoamine oxidase B (MAO-B) inhibition by active principles from Uncaria rhynchophylla.
Hou, Wen-Chi; Lin, Rong-Dih; Chen, Cheng-Tang; Lee, Mei-Hsien
2005-08-22
Attenuation of monoamine oxidase B (MAO-B) activity may provide protection against oxidative neurodegeneration. For this reason, inhibition of MAO-B activity is used as part of the treatment of Parkinson's and Alzheimer's patients. The hook of Uncaria rhynchophylla (Miq.) Jacks. (Rubiaceae) is a traditional Chinese herbal drug that is generally used to treat convulsive disorders. In this study, the fractionation and purification of Uncaria rhynchophylla extracts using a bioguided assay isolated two known compounds, (+)-catechin and (-)-epicatechin. The compounds inhibited MAO-B, as measured by an assay of rat brain MAO-B separated by electrophoresis on a 7.5% native polyacrylamide gel. The IC(50) values of (+)-catechin and (-)-epicatechin were 88.6 and 58.9 microM, respectively, and inhibition occurred in a dose-dependent manner, as measured by the fluorescence method. The Lineweaver-Burk plot revealed K(i) values for (+)-catechin and (-)-epicatechin of 74 and 21 microM, respectively. This suggests that these two compounds, isolated here for the first time from Uncaria rhynchophylla, might be able to protect against neurodegeneration in vitro, and, therefore, the molecular mechanism deserves further study. This finding may also increase interest in the health benefits of Uncaria rhynchophylla.
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Journal of Biosciences | Indian Academy of Sciences
Indian Academy of Sciences (India)
Lineweaver–Burk plot analysis is the most widely used method to determine enzyme kinetic parameters. In the spectrophotometric determination of enzyme activity using the Lineweaver–Burk plot, it is necessary to find a wavelength at which only the substrate or the product has absorbance without any spectroscopic ...
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International Nuclear Information System (INIS)
Barhoumi, H.; Maaref, A.; Rammah, M.; Martelet, C.; Jaffrezic, N.; Mousty, C.; Vial, S.; Forano, C.
2006-01-01
Urea biosensors for medical diagnostic monitoring were developed based on the immobilization of urease within layered double hydroxides (LDH). The urease-LDH material was obtained by a stepwise exchange reaction by urease of a Zn 3 Al-dodecyl sulphate (ZnAl-DS) colloidal suspension. XR diffraction and FTIR analysis show that this method gives rise to a Zn 3 Al-Urease LDH nanohybrid material with urease dispersion and textural properties. An aqueous suspension of this urease-LDH nanohybrid material was deposited on an insulated semiconductor (IS) structure. Biosensor responses to urea additions were obtained using capacitance (C vs. V) and impedance (Z vs. ω) measurements. An enhanced maximum limit of the dynamic range was observed in the case of the impedance measurements (110 mM) compared to (5.6 mM) the capacitive urea biosensor. The Michaelis-Menten constant was also calculated according to the Lineweaver-Burk plot. It was found that the K m value with immobilized enzymes was lower (K m = 0.67 mM) in comparison with free enzymes. This K m value obtained from the capacitance measurements indicates that the urea degradation is performed within any inhibition action on the IS/Zn 3 Al-Urease LDH electrode. A comparative study was carried out between these results and those obtained previously, using urease/ZnAl-Cl layered double hydroxides mixture coated on the pH-ISFET transducer
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Uday, Uma Shankar Prasad; Majumdar, Ria; Tiwari, Onkar Nath; Mishra, Umesh; Mondal, Abhijit; Bandyopadhyay, Tarun Kanti; Bhunia, Biswanath
2017-12-01
In the present work, a potent xylanase producing fungal strain Aspergillus niger (KP874102.1) was isolated through cultural and morphological observations from soil sample of Baramura forest, Tripura west, India. 28S rDNA technique was applied for genomic identification of this fungal strain. The isolated strain was found to be phylogenetically closely related to Aspergillus niger. Kinetic constants such as K m and V max for extracellular xylanase were determined using various substrate such as beech wood xylan, oat spelt xylan and CM cellulose through Lineweaver-Burk plot. K m , V max and K cat for beech wood xylan are found to be 2.89mg/ml, 2442U and 426178Umlmg -1 respectively. Crude enzyme did not show also CM cellulose activity. The relative efficiency of oat spelt xylan was found to be 0.819 with respect to beech wood xylan. After acid hydrolysis, enzyme was able to produce reducing sugar with 17.7, 35.5, 50.8 and 65% (w/w) from orange peel after 15, 30, 45 and 60min incubation with cellulase free xylanase and maximum reducing sugar formation rate was found to be 55.96μg/ml/min. Therefore, the Aspergillus niger (KP874102.1) is considered as a potential candidate for enzymatic hydrolysis of orange peel. Copyright © 2017 Elsevier B.V. All rights reserved.
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Inhibition of monoamine oxidase B (MAO-B) by Chinese herbal medicines.
Lin, R D; Hou, W C; Yen, K Y; Lee, M H
2003-11-01
Monoamine oxidase (MAO) catalyzes the oxidative deamination of biogenic amines accompaned by the release of H2O2. Two subtypes, MAO-A and MAO-B, exist on the basis of their specificities to substrates and inhibitors. The regulation of MAO-B activity is important in the treatment of neurodegenerative diseases. Twenty-seven species of plants used in traditional Chinese medicines, selected from an enthnobotanical survey, were used in an investigation of their inhibitory effect on MAO-B in rat brain homogenates. The 50% aqueous methanol extracts of four active extracts, Arisaema amurense, Lilium brownii var. colchesteri, Lycium chinense, and Uncaria rhynchophylla, exhibited the best activity and selectivity towards MAO-B with IC50 values of 0.44, 0.29, 0.40, and 0.03 mg/ml, respectively. A kinetic study of MAO-B inhibition by the four extracts using the Lineweaver-Burk plot for each active extract revealed the IC50 concentrations, and results show that: Ki = 0.59 mg/ml for A. amurense for the mixed-type mode, Ki = 0.58 mg/ml for L. brownii var. colchesteri for the mixed-type mode, Ki = 5.01 mg/ml for L. chinense for the uncompetitive mode, and Ki = 0.02 mg/ml for U. rhynchophylla for the uncompetitive mode. These may therefore be candidates for use in delaying the progressive degeneration caused by neurological diseases.
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Traditionalism and modern subjectivity: Enlightenment and conservatism of Edmund Burke
Directory of Open Access Journals (Sweden)
Nikitović Aleksandar
2003-01-01
Full Text Available The issue of traditionalism versus modern subjectivism in the light of the conflict of Edmund Burke`s conservatism with the Enlightenment as the ideological basis of the French revolution was not discussed or studied sufficiently in our political and philosophical theory. In this paper we are reconsidering a theoretical debate between arising modern rationalism of Enlightenment and European traditionalism. The text further explains on the reasons for choosing this subject and course the research will take subsequently. An overview is provided of the most important topics as well as a preliminary conception of the problem structure in this research. Considering the importance and overwhelming influence exhorted by French revolution it is only logical that the event of such magnitude met not only with approvals but with challenges and disputes as well, and all of it contributed to the theoretical considerations of the historical sense and purpose of this event. The aim of the project is to provide explanations and evaluations of the origins Enlightenment on one hand side and theoretically reconsidered traditionalism on the other. Looking at the dawn of modern era and evaluating these events would cast sharper light on the spirit of times of this crucial moment of history, and it would provide new basis for reconsideration of the achievements and implications of the fundamental ideas of Moderna.
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Yazdi, Fatemeh; Minai-Tehrani, Dariush; Jahngirvand, Mahboubeh; Almasirad, Ali; Mousavi, Zahra; Masoud, Masoudeh; Mollasalehi, Hamidreza
2015-06-01
In erythrocyte, catalase plays an important role to protect cells from hydrogen peroxide toxicity. Hydrogen peroxide is a byproduct compound which is produced during metabolic pathway of cells. Cimetidine, a histamine H2 receptor antagonist, is used for gastrointestinal tract diseases and prevents the extra release of gastric acid. In this study, the effect of cimetidine on the activity of human erythrocyte catalase was investigated. Erythrocytes were broken by hypotonic solution. The supernatant was used for catalase assay and kinetics study. Lineweaver-Burk plot was performed to determine the type of inhibition. The kinetics data revealed that cimetidine inhibited the catalase activity by mixed inhibition. The IC50 (1.54 μM) and Ki (0.45 μM) values of cimetidine determined that the drug was bound to the enzyme with high affinity. Circular dichroism and fluorescence measurement showed that the binding of cimetidine to the enzyme affected the content of secondary structure of the enzyme as well as its conformational changes. Docking studies were carried out to detect the site in which the drug was bound to the enzyme. Molecular modeling and energy calculation of the binding showed that the cyanoguanidine group of the drug connected to Asp59 via two hydrogen bonds, while the imidazole group of the drug interacted with Phe64 in the enzyme by a hydrophobic interaction. In conclusion, cimetidine could bind to human erythrocyte catalase, and its interaction caused functional and conformational changes in the enzyme.
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Implementing "Abbott v. Burke": A Guide to the 2006 K-12 Abbott Regulations
Education Law Center, 2005
2005-01-01
Except for school construction, there is no legislation to guide implementation of the programs and reforms ordered by the New Jersey Supreme Court in the landmark "Abbott v. Burke" case. Instead, in its 1998 "Abbott V decision," the Supreme Court directed the Commissioner of Education to provide standards and procedures to…
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International Nuclear Information System (INIS)
Quail, J.A.; Jellinck, P.H.
1987-01-01
The ability of GH from various mammalian species, administered to normal mature male rats by constant infusion, to decrease the hepatic 2-hydroxylation of estradiol (E2) to female levels, as measured by the release of 3 H 2 O from [2-3H]E2, was determined. Rat and human GH (hGH) showed the highest activity while ovine GH was inactive. PRL (0.6 IU/h X kg) administered together with hGH (0.02 IU/h X kg) did not antagonize the feminizing action of GH. Infusion of hGH into male rats decreased the affinity of estradiol 2-hydroxylase for its steroid substrate and altered the linear Lineweaver-Burk plot towards a nonlinear hyperbolic plot characteristic of the female. The apparent Michaelis-Menten constant (Km) for the reaction was 1.69 microM for males and 2.75 microM for testosterone-treated ovariectomized females. An equal mixture of liver microsomes from male and female rats gave kinetic values similar to those observed with males alone. Neonatal imprinting with androgen did not alter the magnitude of the response of female rats to treatment with testosterone and/or GH at maturity and the androgen effect could only be shown in ovariectomized animals. The results with rats of different endocrine status were corroborated by the kinetic data and by the pattern of metabolites obtained with [4- 14 C]E2 when examined by TLC and autoradiography. The hormonal control of estradiol 2-hydroxylase, the key enzyme in catechol estrogen formation, and the contribution of sex-specific multiple forms of the enzyme to this reaction are discussed
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Rhetorical Interpretation of Abstracts in Sci-Tech Theses Based on Burke's Identification Theory
Zhong, Jihong
2017-01-01
Abstract of a thesis is the brief and accurate representation of the thesis, with the important function of persuading readers to read on the thesis. So how the writer constructs the abstract and wins readers' recognition is our main focus. On the basis of Burke's Identification Theory, this paper analyzed 10 abstracts from "Nature" from…
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Rodrigues, Nils; Weiskopf, Daniel
2018-01-01
Conventional dot plots use a constant dot size and are typically applied to show the frequency distribution of small data sets. Unfortunately, they are not designed for a high dynamic range of frequencies. We address this problem by introducing nonlinear dot plots. Adopting the idea of nonlinear scaling from logarithmic bar charts, our plots allow for dots of varying size so that columns with a large number of samples are reduced in height. For the construction of these diagrams, we introduce an efficient two-way sweep algorithm that leads to a dense and symmetrical layout. We compensate aliasing artifacts at high dot densities by a specifically designed low-pass filtering method. Examples of nonlinear dot plots are compared to conventional dot plots as well as linear and logarithmic histograms. Finally, we include feedback from an expert review.
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BliP PLOT : PLOT DISTRIBUSI DATA BERDIMENSI - SATU
Anisa Anisa; Indwiati Indwiati
2014-01-01
Blip Plot, adalah salah satu plot yang sibuat untuk menampilkan data berdimensi-satu. Pada dasarnya plot ini terdiri dari kotak, garis, dan titik. Sebagaimana plot distribusi berdimensi-satu yang lain, BliP Plot menampilkan nilai-nilai data individu dalam titik-titik atau garis-garis, dan informasi berkelompok dalam garis atau kotak. Kelebihannya, Blip Plot menampilkan banyak keistimewaan baru seperti plot variable-widht dan beberapa pilihan pola titik. Keuntungan utama dari Blip ...
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Picot, Marie Carene Nancy; Bender, Onur; Atalay, Arzu; Zengin, Gokhan; Loffredo, Loïc; Hadji-Minaglou, Francis; Mahomoodally, Mohamad Fawzi
2017-05-01
Aphloia theiformis (Vahl.) Benn. (AT) is traditionally used in Sub-Saharan African countries including Mauritius as a biomedicine for the management of several diseases. However, there is a dearth of experimental studies to validate these claims. We endeavoured to evaluate the inhibitory effects of crude aqueous extract as traditionally used together with the crude methanol extracts of AT leaves on urease, angiotensin (I) converting enzyme (ACE), acetylcholinesterase (AChE), cholesterol esterase (CEase), glycogen phosphorylase a (GPa), and glycation in vitro. The crude extract showing potent activity against the studied enzymes was further partitioned using different solvents of increasing polarity. The enzyme inhibitory and antiglycation activities of each fraction was assessed. Kinetic of inhibition of the active crude extract/fractions on the aforementioned enzymes was consequently determined using Lineweaver-Burk plots. An ultra-high performance liquid chromatography (UHPLC-UV/MS) system was used to establish the phytochemical profile of AT. The real time cell analysis system (iCELLigence™) was used to monitor any cellular cytotoxicity of AT. Crude methanolextract (CME) was a potent inhibitor of the studied enzymes, with IC 50 ranging from 696.22 to 19.73μg/mL. CME (82.5%) significantly (p20μg/mL) according to the U.S National Cancer Institute plant screening program. This study has established baseline data that tend to justify the traditional use of AT and open new avenues for future biomedicine development. Copyright © 2017 Elsevier Masson SAS. All rights reserved.
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Li, Cuiping; Liu, Youxun; Fu, Yun; Huang, Tengfei; Kang, Lixia; Li, Changzheng
2017-08-22
The bioactivity of drugs is attributed to their interaction with biological molecules, embodied in either their direct or indirect influence on enzyme activity and conformation. Di-2-pyridylketone hydrazine dithiocarbamate (DpdtC) exhibits significant antitumor activity in our preliminary study. We speculated that its activity may partly stem from enzyme inhibition due to strong metal chelating ability. To this end, we assessed its effect on catalase from erythrocytes and found evidence of inhibition, which was further confirmed by ROS determination in vivo. Thus, detailing the interaction between the agent and catalase via spectroscopic methods and molecular docking was required to obtain information on both the dynamics and thermodynamic parameters. The Lineweaver-Burk plot implied an uncompetitive pattern between DpdtC and catalase from beef liver, and IC 50 = ∼7 μM. The thermodynamic parameters from fluorescence quenching measurements indicated that DpdtC could bind to catalase with moderate affinity (K a = approximately 10 4 M -1 ). CD spectra revealed that DpdtC could significantly disrupt the secondary structure of catalase. Docking studies indicated that DpdtC bound to a flexible region of catalase, involving hydrogen bonds and salt bond; this was consistent with thermodynamic results from spectral investigations. Our data clearly showed that catalase inhibition of DpdtC was not due to direct chelation of iron from heme (killing), but through an allosteric effect. Thus, it can be concluded that the antiproliferative activity of DpdtC is partially attributed to its catalase inhibition.
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Directory of Open Access Journals (Sweden)
Nikolay Avtandilyan
2018-01-01
Full Text Available It is well established that, during development of malignancies, metabolic changes occur, including alterations of enzyme activities and isoenzyme expression. Arginase and nitric oxide (NO synthase (NOS are two of those enzymes considered to be involved in tumorigenesis. The goal of this article was to study the involvement of arginase and NOS in the development of different stages of breast cancer. Our results have shown that human serum arginase activity and NO (resp., and NOS activity and polyamines quantities increased in parallel with cancer stage progression and decreased after neoadjuvant chemotherapy. For breast cancer, the only isoenzyme of arginase expressed in serum before and after chemotherapy was in a cationic form. The data of Lineweaver-Burk plot with a Km value of 2 mM was calculated, which is characteristic for human liver type isoform of arginase. During electrophoresis at pH 8.9, the enzyme exhibited high electrophoretic mobility and was detected near the anode. The presented results demonstrated that arginase in human serum with breast cancer and after chemotherapy is not polymorphic. We suggest that arginase and NOS inhibition has antitumor effects on cancer development, as it can inhibit polyamines and NO levels, a precursor of cancer cell proliferation, metastasis, and tumor angiogenesis.
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Bufadienolides from Kalanchoe daigremontiana as thrombin inhibitors-In vitro and in silico study.
Kolodziejczyk-Czepas, Joanna; Sieradzka, Malgorzata; Moniuszko-Szajwaj, Barbara; Pecio, Łukasz; Ponczek, Michal B; Nowak, Pawel; Stochmal, Anna
2017-06-01
Thrombin is an active plasma coagulation factor II, critical for the formation of fibrin clot during blood coagulation. For that reason, this protein is also a crucial target for different anti-thrombotic therapies. The work is based on in vitro evaluation of the inhibitory effect of bufadienolide-rich fraction, isolated from roots of Kalanchoe daigremontiana (1-50μg/ml) on enzymatic properties of a serine proteinase - thrombin. The efficacy of the inhibition of amidolytic activity of thrombin (measured as a hydrolysis of the chromogenic substrate S-2238, Chromogenix) attained about 10 and 66%, respectively. The IC 50 , established for the examined bufadienolide fraction was 2.79μg/ml, while the IC 50 calculated for argatroban (reference compound) was 0.78μg/ml. Linearization conducted using Lineweaver-Burk plot indicated that the K. daigremontiana fraction contains compounds that are uncompetitive inhibitors of thrombin. K. daigremontiana fraction was also able to reduce the proteolytic activity of thrombin towards its physiological substrate, i.e. fibrinogen. Additionally, this study is supported by in silico analysis of interactions of the most common compounds, identified in the examined in Kalanchoe extract to crystal structure of this enzyme. Copyright © 2017 Elsevier B.V. All rights reserved.
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Some effects of lead at mammalian neuromuscular junction
Energy Technology Data Exchange (ETDEWEB)
Pickett, J.B.; Bornstein, J.C.
1984-03-01
The effect of lead on transmitter release was investigated in a rat phrenic nerve-hemidiaphragm preparation using conventional microelectrode techniques. Lead reduced the number of quanta released by a nerve stimulus (m) in a dose-dependent fashion. As extracellular Ca/sup 2 +/ concentration ((Ca/sup 2 +/)/sub 0/) was varied in the absence of lead, a linear relationship between ln(m) and ln((Ca/sup 2 +/)/sub 0/) was obtained. Lead shifted the relationship between ln(m) and ln((Ca/sup 2 +/)/sub 0/) to the right without altering the slope. This suggested lead competed with Ca/sup 2 +/, which was confirmed by using a modified Lineweaver-Burk plot. Lead inhibits Ca/sup 2 +/ entry into frog sympathetic preganglionic nerve terminals, and a similar mechanism may underlie this present finding; such a mechanism, however, could not explain all the observed actions of lead. Lead increased the frequency of spontaneous quantal release in a dose-dependent manner, and 10/sup -4/ M lead doubled the magnitude of facilitation of evoked release seen with five stimuli at 60 Hz. It is suggested that these effects result from inhibition of some, or all, of the nerve terminal's Ca/sup 2 +/ sequestration mechanisms.
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2013-08-16
... with the appropriate Indian tribes or Native Hawaiian organizations, has determined that the cultural... organization not identified in this notice that wish to claim these cultural items should submit a written....R50000] Notice of Intent To Repatriate Cultural Items: Thomas Burke Memorial Washington State Museum...
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Directory of Open Access Journals (Sweden)
Muhammad Athar Abbasi
2018-06-01
Full Text Available The present study comprises the synthesis of a new series of sulfonamides derived from 4-methoxyphenethylamine (1. The synthesis was initiated by the reaction of 1 with 4-methylbenzenesulfonyl chloride (2 in aqueous sodium carbonate solution at pH 9 to yield N-(4-methoxyphenethyl-4-methylbenzensulfonamide (3.This parent molecule 3 was subsequently treated with various alkyl/aralkyl halides, (4a–j, using N,N-dimethylformamide (DMF as solvent and LiH as activator to produce a series of new N-(4-methoxyphenethyl-N-(substituted-4-methylbenzenesulfonamides (5a–j. The structural characterization of these derivatives was carried out by spectroscopic techniques like IR, 1H-NMR, and 13C-NMR. The elemental analysis data was also coherent with spectral data of these molecules. The inhibitory effects on acetylcholinesterase and DPPH were evaluated and it was observed that N-(4-Methoxyphenethyl-4-methyl-N-(2-propylbenzensulfonamide (5c showed acetylcholinesterase inhibitory activity 0.075 ± 0.001 (IC50 0.075 ± 0.001 µM comparable to Neostigmine methylsulfate (IC50 2.038 ± 0.039 µM.The docking studies of synthesized ligands 5a–j were also carried out against acetylcholinesterase (PDBID 4PQE to compare the binding affinities with IC50 values. The kinetic mechanism analyzed by Lineweaver-Burk plots demonstrated that compound (5c inhibits the acetylcholinesterase competitively to form an enzyme inhibitor complex. The inhibition constants Ki calculated from Dixon plots for compound (5c is 2.5 µM. It was also found from kinetic analysis that derivative 5c irreversible enzyme inhibitor complex. It is proposed on the basis of our investigation that title compound 5c may serve as lead structure for the design of more potent acetylcholinesterase inhibitors.
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"If Your Roommate Dies, You Get A 4.0": Reclaiming Rumor with Burke and Organizational Culture.
Scheibel, Dean
1999-01-01
Argues for a reconsideration of rumor from an interpretive perspective and articulates a theoretical perspective that combines organizational culture with Burke's guilt-purification-redemption cycle. Concludes that students use rumor to make sense of and to cope with problematic aspects of their university-life pertaining to roommates, academic…
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Interaction of tea polyphenols with serum albumins: A fluorescence spectroscopic analysis
Energy Technology Data Exchange (ETDEWEB)
Bose, Adity, E-mail: adityc17j@gmail.com
2016-01-15
Interactions of some tea polyphenols, namely (−) Catechin (C), (−)-epicatechin (EC), (–) epicatechin-3-gallate (ECG), (−)-epigallocatechin (EGC) and (−)-epigallocatechin-3-gallate (EGCG) are outlined with the serum albumin proteins. These interactions had all resulted in binding with the proteins with a concomitant static quenching of the protein fluorescence. A fluorescence technique has been considered as the tool to comprehend the polyphenol–protein interactions mainly and simultaneously other spectroscopic techniques used to verify the results have been discussed. In this mini review the different types of equations usually employed to calculate the binding constant values have been outlined, namely, modified Stern Volmer plot, Scatchard plot and Lineweaver Burk equation, with their corresponding results. The n values (number of binding sites) had always been close to unity suggesting a 1:1 complexation with the polyphenols and the protein. A structural change in the polyphenols has been found to alter the binding constant value and the galloyl moiety attached to the C ring of the polyphenols have been found to play a crucial role in this regard. It has been found that an increase in galloyl moiety increases binding of the catechins with proteins. - Highlights: • Review on interactions of some tea polyphenols with the serum albumin proteins. • Tea polyphenols include Catechin, epigallocatechin-3-gallate, epigallocatechin, epicatechin-3-gallate and epicatechin. • Fluorescence spectroscopic technique is mainly outlined. • Binding constant studies have been given importance. • Galloyl moiety in the C ring is crucial in increasing binding constant.
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Wang, Ting; Guo, Rixin; Zhou, Guohong; Zhou, Xidan; Kou, Zhenzhen; Sui, Feng; Li, Chun; Tang, Liying; Wang, Zhuju
2016-07-21
Panax notoginseng (Burk.) F.H. Chen is a widely used traditional Chinese medicine known as Sanqi or Tianqi in China. This plant, which is distributed primarily in the southwest of China, has wide-ranging pharmacological effects and can be used to treat cardiovascular diseases, pain, inflammation and trauma as well as internal and external bleeding due to injury. This paper provides up-to-date information on investigations of this plant, including its botany, ethnopharmacology, phytochemistry, pharmacology and toxicology. The possible uses and perspectives for future investigation of this plant are also discussed. The relevant information on Panax notoginseng (Burk.) F.H. Chen was collected from numerous resources, including classic books about Chinese herbal medicine, and scientific databases, including Pubmed, SciFinder, ACS, Ebsco, Elsevier, Taylor, Wiley and CNKI. More than 200 chemical compounds have been isolated from Panax notoginseng (Burk.) F.H. Chen, including saponins, flavonoids and cyclopeptides. The plant has pharmacological effects on the cardiovascular system, immune system as well as anti-inflammatory, anti-atherosclerotic, haemostatic and anti-tumour activities, etc. Panax notoginseng is a valuable traditional Chinese medical herb with multiple pharmacological effects. This review summarizes the botany, ethnopharmacology, phytochemistry, pharmacology and toxicology of P. notoginseng, and presents the constituents and their corresponding chemical structures found in P. notoginseng comprehensively for the first time. Future research into its phytochemistry of bio-active components should be performed by using bioactivity-guided isolation strategies. Further work on elucidation of the structure-function relationship among saponins, understanding of multi-target network pharmacology of P. notoginseng, as well as developing its new clinical usage and comprehensive utilize will enhance the therapeutic potentials of P. notoginseng. Copyright © 2016
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Mcentire, K.
1994-01-01
NPLOT is an interactive computer graphics program for plotting undeformed and deformed NASTRAN finite element models (FEMs). Although there are many commercial codes already available for plotting FEMs, these have limited use due to their cost, speed, and lack of features to view BAR elements. NPLOT was specifically developed to overcome these limitations. On a vector type graphics device the two best ways to show depth are by hidden line plotting or haloed line plotting. A hidden line algorithm generates views of models with all hidden lines removed, and a haloed line algorithm displays views with aft lines broken in order to show depth while keeping the entire model visible. A haloed line algorithm is especially useful for plotting models composed of many line elements and few surface elements. The most important feature of NPLOT is its ability to create both hidden line and haloed line views accurately and much more quickly than with any other existing hidden or haloed line algorithms. NPLOT is also capable of plotting a normal wire frame view to display all lines of a model. NPLOT is able to aid in viewing all elements, but it has special features not generally available for plotting BAR elements. These features include plotting of TRUE LENGTH and NORMALIZED offset vectors and orientation vectors. Standard display operations such as rotation and perspective are possible, but different view planes such as X-Y, Y-Z, and X-Z may also be selected. Another display option is the Z-axis cut which allows a portion of the fore part of the model to be cut away to reveal details of the inside of the model. A zoom function is available to terminals with a locator (graphics cursor, joystick, etc.). The user interface of NPLOT is designed to make the program quick and easy to use. A combination of menus and commands with help menus for detailed information about each command allows experienced users greater speed and efficiency. Once a plot is on the screen the interface
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The Rule of Law against the Rule of Greed: Edmund Burke against the East India Company
Directory of Open Access Journals (Sweden)
Jakob Fortunat Stagl
2012-01-01
Full Text Available At the end of the 18th century Bengal suddenly came under the rule of the East India Company. The former trading company had become the sovereign, first, of a country the size of France, and eventually of the whole Indian subcontinent. The Company was not controlled by any positive law, be it Indian, British or international. As a consequence, the Company’s individual and corporate greed reigned supreme, with the most dire consequences for the native Indian population. The Indian question aroused the interest of Edmund Burke. He saw in India a metaphor for his native Ireland and was suspicious of the corruption of British politics by the money and influence that the Company’s men had gained in India. He therefore made it the aim of his life to fight the Company’s unrestrained avarice by fostering an impeachment trial against Warren Hastings, the first Governor General of Bengal. In order to get Hastings convicted it was necessary to show that he had infringed the law. But which law should Hastings’ judges apply? He resorted to Natural Law and Roman Law. Thence he took the maxim »Eundem negotiatorem et dominum«, that is to say, commerce which aims at profit, and government which aims at the welfare of the population, are irreconcilable. Though after many years Hastings was acquitted, Burke contributed by this trial to civilising British rule in India. Burke’s stance has recently been criticised by the post-colonial school: He should have pleaded for the British to quit India rather than improving their rule and thereby prolonging its existence.
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Demir, Hülya; Ciftçi, Mehmet; Küfrevioğlu, O Irfan
2003-02-01
In this study, 6-phosphogluconate dehydrogenase (E.C.1.1.44; 6PGD) was purified from parsley (Petroselinum hortense) leaves, and analysis of the kinetic behavior and some properties of the enzyme were investigated. The purification consisted of three steps that are preparation of homogenate ammonium sulfate fractionation and on DEAE-Sephadex A50 ion exchange. The enzyme was obtained with a yield of 49% and had a specific activity of 18.3 U (mg proteins)(-1) (Lehninger, A.L.; Nelson, D.L.; Cox, M.M. Principles of Biochemistry, 2nd Ed.; Worth Publishers Inc.: N.Y., 2000, 558-560). The overall purification was about 339-fold. A temperature of +4 degrees C was maintained during the purification process. Enzyme activity was spectrophotometrically measured according to the Beutler method at 340 mn. In order to control the purification of the enzyme, SDS-polyacrylamide gel electrophoresis was carried out in 4% and 10% acrylamide for stacking and running gel, respectively. SDS-polyacrylamide gel electrophoresis showed a single band for enzyme. The molecular weight was found to be 97.5 kDa by Sephadex G-150 gel filtration chromatography. A protein band corresponding to a subunit molecular weight of 24.1 kDa was obtained on SDS-polyacrylamide gel electrophoresis. For the enzymes, the stable pH, optimum pH, and optimum temperature were found as 8.0, 8.0, and 50 degrees C, respectively. In addition, KM and Vmax values for NADP+ and G6-P at optimum pH and 25 degrees C were determined by means of Lineweaver-Burk plots.
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Biosynthesis, purification and characterization of commercial enzyme by penicillium expansum link
International Nuclear Information System (INIS)
Ahmed, K.; Valeem, E.E.
2015-01-01
Ever growing biotechnological industry has motivated the research towards the comprehensive survey of microorganisms, which could be used in extreme conditions of industry. In the present work optimization parameters in submerged fermentation, purification and characterization of invertase from Penicillium expansum Link using agricultural wastes (sunflower waste, cotton stalk and rice husk) as well as agro-industrial wastes (date syrup and molasses) as sources of carbon. Maximum production of invertase (7.03 U/mL) was observed when the strain was grown on culture medium (CM1) containing yeast extract as a source of nitrogen, date syrup as a source of carbon after 48 h of incubation at initial pH 5.0, temperature 35 degree C, inoculum size of 6x106 conidia in 50 mL of culture medium and agitation rate of 150 rev/min. After optimization the enzyme was also purified partially and then characterized. Kinetic constants (Km 2.57 mM and Vmax 178.6 U/mL/min) were determined by Lineweaver-Burk Plot and molecular mass (110 kDa) by 10% SDS-PAGE. Invertase showed maximum activity at pH 5.5 (128.7 U/mL) and at the temperature of 60 degree C (114.6 U/mL). BaCl/sub 2/ (21.9%), MgSO/sub 4/ (42.6%), MnCl/sub 2/ (46.8%) and EDTA (8.3%) enhanced the relative activity of enzyme while HgCl2 (-90.9%), CuSO/sub 4/ (-82.3%) and CuCl/sub 2/ (-78.7%) were proved inhibitors. (author)
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Directory of Open Access Journals (Sweden)
Bechan Sharma
2011-01-01
Full Text Available Phosphofructokinase (PFK, a regulatory enzyme in glycolytic pathway, has been purified to electrophoretic homogeneity from adult female Setaria cervi and partially characterized. For this enzyme, the Lineweaver-Burk's double reciprocal plots of initial rates and D-fructose-6-phosphate (F-6-P or Mg-ATP concentrations for varying values of cosubstrate concentration gave intersecting lines indicating that Km values for F-6-P (1.05 mM and ATP (3 μM were independent of each other. S. cervi PFK, when assayed at inhibitory concentration of ATP (>0.1 mM, exhibited sigmoidal behavior towards binding with F-6-P with a Hill coefficient (n value equal to 1.8 and 1.7 at 1.0 and 0.33 mM ATP, respectively. D-fructose-1,6-diphosphate (FDP competitively inhibited the filarial enzyme: Ki and Hill coefficient values being 0.18 μM and 2.0, respectively. Phosphoenolpyruvate (PEP also inhibited the enzyme competitively with the Ki value equal to 0.8 mM. The Hill coefficient values (>1.5 for F-6-P (at inhibitory concentration of ATP and FDP suggested its positive cooperative kinetics towards F-6-P and FDP, showing presence of more than one binding sites for these molecules in enzyme protein and allosteric nature of the filarial enzyme. The product inhibition studies gave us the only compatible mechanism of random addition process with a probable orientation of substrates and products on the enzyme surface.
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Maitra-Burke example of initial-state dependence in time-dependent density-functional theory
International Nuclear Information System (INIS)
Holas, A.; Balawender, R.
2002-01-01
In a recent paper, Maitra and Burke [Phys. Rev. A 63, 042501 (2001); 64, 039901(E) (2001)] have given an interesting and instructive example that illustrates a specific feature of the time-dependent density-functional theory--the dependence of the reconstructed time-dependent potential not only on the electron density, but also on the initial state of the system. However, a concise form of its presentation by these authors is insufficient to reveal all its peculiarities. Our paper represents a very detailed study of this valuable example, intended to facilitate a better understanding and appreciation
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Effect of ions on the activity of brain acetylcholinesterase from tropical fish
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Caio Rodrigo Dias Assis
2015-07-01
Full Text Available Objective: To investigate the effect of ions on brain acetylcholinesterase (AChE; EC 3.1.1.7 activities from economic important fish [pirarucu, Arapaima gigas; tambaqui, Colossoma macropomum; cobia, Rachycentron canadum (R. canadum and Nile tilapia, Oreochromis niloticus (O. niloticus] comparing with a commercial enzyme from electric eel [Electrophorus electricus (E. electricus]. Methods: The in vitro exposure was performed at concentrations ranging from 0.001 to 10 mmol/L (except for ethylene diamine tetraacetic acid; up to 150 mmol/L. Inhibition kinetics on R. canadum and O. niloticus were also observed through four methods (Michaelis-Menten, Lineweaver-Burk, Dixon and Cornish-Bowden plots in order to investigate the type of inhibition produced by some ions. Results: Hg 2+ , As 3+ , Cu 2+ , Zn 2+ , Cd 2+ caused inhibition in all the species under study. Ca 2+ , Mg 2+ and Mn 2+ induced slight activation in R. canadum enzyme while Pb 2+ , Ba 2+ , Fe 2+ , Li + inhibited the AChE from some of the analyzed species. The lowest IC 50 and Ki values were estimated for E. electricus AChE in presence of Hg 2+ , Pb 2+ , Zn 2+ . Under our experimental conditions, the results for R. canadum and O. niloticus, As 3+ , Cu 2+ , Cd 2+ , Pb 2+ and Zn 2+ showed a non- competitive/mixed-type inhibition, while Hg 2+ inhibited the enzyme in a mixed/competitive- like manner. Conclusions: E. electricus AChE activity was affected by ten of fifteen ions under study showing that this enzyme could undergo interference by these ions when used as pesticide biosensor in environmental analysis. This hindrance would be less relevant for the crude extracts.
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Sabiu, Saheed; Ajani, Emmanuel Oladipo; Sunmonu, Taofik Olatunde; Ashafa, Anofi Omotayo Tom
2017-01-01
The continuous search for new lead compounds as viable inhibitors of specific enzymes linked to carbohydrate metabolism has intensified. Cyperus esculentus L. is one of the therapeutically implicated botanicals against several degenerative diseases including diabetes mellitus. This study evaluated the antioxidant and mechanism(s) of inhibitory potential of aqueous extract of C. esculentus on α-amylase and α-glucosidase in vitro . The extract was investigated for its radical scavenging and hypoglycaemic potentials using standard experimental procedures. Lineweaver-Burke plot was used to predict the manner in which the enzymes were inhibited. The data obtained revealed that the extract moderately and potently inhibited the specific activities of α -amylase and α -glucosidase, respectively. The inhibition was concentration-related with respective IC 50 values of 5.19 and 0.78 mg/mL relative to that of the control (3.72 and 3.55 mg/mL). The extract also significantly scavenged free radicals and the effects elicited could be ascribed to its phytoconstituents. The respective competitive and non-competitive mode of action of the extract is due to its inhibitory potentials on the activities of α -amylase and α -glucosidase. Going forward, in addition to completely characterize the exact compound(s) responsible for the elicited activity in this study, pertinent attention will be given to the in vivo evaluation of the identified constituents.
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Split-plot designs for robotic serial dilution assays.
Buzas, Jeffrey S; Wager, Carrie G; Lansky, David M
2011-12-01
This article explores effective implementation of split-plot designs in serial dilution bioassay using robots. We show that the shortest path for a robot to fill plate wells for a split-plot design is equivalent to the shortest common supersequence problem in combinatorics. We develop an algorithm for finding the shortest common supersequence, provide an R implementation, and explore the distribution of the number of steps required to implement split-plot designs for bioassay through simulation. We also show how to construct collections of split plots that can be filled in a minimal number of steps, thereby demonstrating that split-plot designs can be implemented with nearly the same effort as strip-plot designs. Finally, we provide guidelines for modeling data that result from these designs. © 2011, The International Biometric Society.
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Meng, Yonghong; Su, Anping; Yuan, Shuang; Zhao, Huaguo; Tan, Siyuan; Hu, Chingyuan; Deng, Hong; Guo, Yurong
2016-12-01
This study was designed to investigate the inhibition effect and mechanism of total flavonoids, myricetin and quercetin extracted from Hovenia dulcis Thunb. on α-amylase and α-glucosidase in order to explore the potential use of Hovenia flavonoids in alleviating postprandial hyperglycemia. The results demonstrate that total flavonoids, myricetin, and quercetin were effective inhibitors of α-amylase with IC 50 values of 32.8, 662 and 770 μg ml -1 , respectively. And all three were effective inhibitors of α-glucosidase with IC 50 values of 8, 3 and 32 μg ml -1 , respectively. Enzyme kinetics tests and Lineweaver-Burk results showed the inhibition effects of total flavonoids, myricetin and quercrtin on α-amylase were all reversible and competitive, and the effects on α-glucosidase were all reversible but non-competitive. This study revealed that Hovenia flavonoids, especially myricetin, are effective and promising functional foods in alleviating type 2 diabetes mellitus.
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Chen, Dandan; Wu, Qiong; Wang, Jun; Wang, Qi; Qiao, Heng
2015-01-01
In this work, the fluorescence quenching was used to study the interaction of cyanuric acid (CYA) and uric acid (UA) with bovine serum albumin (BSA) at two different temperatures (283 K and 310 K). The bimolecular quenching constant (Kq), apparent quenching constant (Ksv), effective binding constant (KA) and corresponding dissociation constant (KD), binding site number (n) and binding distance (r) were calculated by adopting Stern-Volmer, Lineweaver-Burk, Double logarithm and overlap integral equations. The results show that CYA and UA are both able to obviously bind to BSA, but the binding strength order is BSA + CYA < BSA + UA. And then, the interactions of CYA and UA with melamine (MEL) under the same conditions were also studied by using similar methods. The results indicates that both CYA and UA can bind together closely with melamine (MEL). It is wished that these research results would facilitate the understanding the formation of kidney stones and gout in the body after ingesting excess MEL.
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9 CFR 108.3 - Preparation of plot plans.
2010-01-01
... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Preparation of plot plans. 108.3... LICENSED ESTABLISHMENTS § 108.3 Preparation of plot plans. Plot plans shall show all of the buildings on a... on the plot plan the use of immediate adjacent properties such as, residential area, pasture, box...
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International Nuclear Information System (INIS)
Martin, T.W.; Wysolmerski, R.B.; Lagunoff, D.
1986-01-01
Phosphatidylcholine labeled in the sn-2 position with 3 H-oleic acid was used to measure PLA 2 in cell sonicates (CS) prepared from confluent cultures of BPEC. Substrate at 10-200 μM was incubated with 5-30 μg of CS protein in HEPES buffer at 37 0 C. A plot of 3 H-oleic acid release vs time was linear and proportional to the amount of CS protein. Lineweaver-Burk plots of the data were linear with V/sub max/ = 22.2 nmole/mg protein/hr and K/sub d/ = 121 μM. Under these conditions, phospholipase C activity was 20-fold lower, and phospholipase A 1 activity was not detectable. PLA 2 activity was pH-dependent with optima at 4.5 and 7.5. Ca ++ was not required for activity, and addition of up to 10 mM Ca ++ to CS in EDTA increased activity by only 10-20%. After centrifugation of CS at 100,000 g for 90 min, 62% of the PLA 2 activity was recovered in the particular fraction. Triton X-100 (0.006-0.4%) inhibited PLA 2 up to 90%, whereas 2 mM deoxycholate produced nearly 3-fold activation. Of several agents tested, bromophenacylbromide (BPB) was the most effective inhibitor. Treatment of CS with BPB at 37 0 C for 30 min produced up to 9% inhibition (K/sub i/ = 5 μM). Phenylmethanesulfonyl fluoride at 200 μm produced 41% inhibition. Quinacrine at 1 mM inhibited PLA 2 by 18%. These data define characteristics of BPEC PLA 2 that should prove useful in studies of the role of this enzyme in specific cellular functions
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Glucose isomerization in simulated moving bed reactor by Glucose isomerase
Directory of Open Access Journals (Sweden)
Eduardo Alberto Borges da Silva
2006-05-01
Full Text Available Studies were carried out on the production of high-fructose syrup by Simulated Moving Bed (SMB technology. A mathematical model and numerical methodology were used to predict the behavior and performance of the simulated moving bed reactors and to verify some important aspects for application of this technology in the isomerization process. The developed algorithm used the strategy that considered equivalences between simulated moving bed reactors and true moving bed reactors. The kinetic parameters of the enzymatic reaction were obtained experimentally using discontinuous reactors by the Lineweaver-Burk technique. Mass transfer effects in the reaction conversion using the immobilized enzyme glucose isomerase were investigated. In the SMB reactive system, the operational variable flow rate of feed stream was evaluated to determine its influence on system performance. Results showed that there were some flow rate values at which greater purities could be obtained.Neste trabalho a tecnologia de Leito Móvel Simulado (LMS reativo é aplicada no processo de isomerização da glicose visando à produção de xarope concentrado de frutose. É apresentada a modelagem matemática e uma metodologia numérica para predizer o comportamento e o desempenho de unidades reativas de leito móvel simulado para verificar alguns aspectos importantes para o emprego desta tecnologia no processo de isomerização. O algoritmo desenvolvido utiliza a abordagem que considera as equivalências entre as unidades reativas de leito móvel simulado e leito móvel verdadeiro. Parâmetros cinéticos da reação enzimática são obtidos experimentalmente usando reatores em batelada pela técnica Lineweaver-Burk. Efeitos da transferência de massa na conversão de reação usando a enzima imobilizada glicose isomerase são verificados. No sistema reativo de LMS, a variável operacional vazão da corrente de alimentação é avaliada para conhecer o efeito de sua influência no
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Directory of Open Access Journals (Sweden)
Sharifi Mahbobeh
2011-01-01
Full Text Available Xanthogaleruca luteola Müll. (Col.: Chrysomelidae is a major urban insect pest on elm trees in Iran. Digestion in the alimentary canal of the elm leaf beetle is facilitated by some carbohydrases which are responsible for the digestion of carbohydrates. The presence of digestive carbohydrases was determined in the digestive system of adult and last larval instar of the elm leaf beetle. The specific activity of α-amylase in the digestive system of adult females and last larval instars were 0.49± 0.05 and 0.72± 0.07 μmol/min/mg protein, respectively. Also, the amylase activity in the midgut of the last larval instar was 3.125- and 4.16-fold higher than that its activity in the foregut and hindgut, respectively. Results showed that optimum activity for α-amylase was found at pH 5. As calculated from Lineweaver-Burk plots, the Km values for α-amylase were 0.64 and 1.44 mg/ml, when glycogen and starch were used as substrates, respectively. The effect of pH and temperature on α- and ß-glucosidase and α- and β-galactosidase activities was determined in the digestive system of X. luteola. Results showed that the activity of α- and ß-glucosidases in adult females was higher than in larvae, but the Яgalactosidase activity in larvae was more than that of the adult. In adult females the glucosidase activity was higher than the galactosidase activity. The zymogram pattern in the native gel revealed that X. luteola α-amylase, β-glucosidase and β-galactosidase in the digestive system had one, three and one isoforms. α-amylase inhibitors, purified from Phaseolus vulgaris L. with an ion-exchange DEAE cellulose column showed good inhibitory activity on X. luteola gut α-amylase.
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The rainfall plot: its motivation, characteristics and pitfalls.
Domanska, Diana; Vodák, Daniel; Lund-Andersen, Christin; Salvatore, Stefania; Hovig, Eivind; Sandve, Geir Kjetil
2017-05-18
A visualization referred to as rainfall plot has recently gained popularity in genome data analysis. The plot is mostly used for illustrating the distribution of somatic cancer mutations along a reference genome, typically aiming to identify mutation hotspots. In general terms, the rainfall plot can be seen as a scatter plot showing the location of events on the x-axis versus the distance between consecutive events on the y-axis. Despite its frequent use, the motivation for applying this particular visualization and the appropriateness of its usage have never been critically addressed in detail. We show that the rainfall plot allows visual detection even for events occurring at high frequency over very short distances. In addition, event clustering at multiple scales may be detected as distinct horizontal bands in rainfall plots. At the same time, due to the limited size of standard figures, rainfall plots might suffer from inability to distinguish overlapping events, especially when multiple datasets are plotted in the same figure. We demonstrate the consequences of plot congestion, which results in obscured visual data interpretations. This work provides the first comprehensive survey of the characteristics and proper usage of rainfall plots. We find that the rainfall plot is able to convey a large amount of information without any need for parameterization or tuning. However, we also demonstrate how plot congestion and the use of a logarithmic y-axis may result in obscured visual data interpretations. To aid the productive utilization of rainfall plots, we demonstrate their characteristics and potential pitfalls using both simulated and real data, and provide a set of practical guidelines for their proper interpretation and usage.
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Natural and synthetic oviposition stimulants forCatolaccus grandis (Burks) females.
Guerra, A A; Martinez, S; Sonia Del Rio, H
1994-07-01
Oviposition behavior was elicited fromCatolaccus grandis (Burks) (Hymenoptera: Pteromalidae) females, an ectoparasitoid of the boll weevil,Anthonomus grandis Boheman (Coleoptera: Curculionidae), by smears of freshly cut cotton bolls or smears of extracts prepared with boll weevil damaged or undamaged cotton boll tissues. Oviposition behavior was also elicited fromC. grandis females by smears made withn-pentane,n-hexane,n-heptane, and isooctane. This is the first report of oviposition behavior elicited for any parasitoid by these short-chain saturated hydrocarbons (alkanes), introducing a new concept on the chemical mediation of parasitoid behavior during host selection. Oviposition behavior was also elicited fromC. grandis females by volatiles emanating from an artificial diet devoid of insect components that was specifically developed for the in vitro rearing of ectoparasitoids. The possible use of a synergistic combination ofn-hexane and diet to optimize the mechanized production of noncontaminated eggs is also discussed.
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La Révolution française interprète de Burke et Kant
Directory of Open Access Journals (Sweden)
Yannick Bosc
2004-06-01
Full Text Available Les catégories de « conservatisme » ou de « modernité », à partir desquelles on classe toute chose, ne se révèlent que rarement pertinentes lorsqu’on observe de près. Ainsi en fonction des exégètes ou suivant les moments considérés, Kant et Burke peuvent être tour à tour « conservateurs » ou « progressistes ». Dans un ouvrage synthétique qui ambitionne d’échapper à cette alternative, Benjamin Delannoy remet en chantier les travaux des quinze dernières ...
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Worm plot to diagnose fit in quantile regression
Buuren, S. van
2007-01-01
The worm plot is a series of detrended Q-Q plots, split by covariate levels. The worm plot is a diagnostic tool for visualizing how well a statistical model fits the data, for finding locations at which the fit can be improved, and for comparing the fit of different models. This paper shows how the
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Worm plot to diagnose fit in quantile regression
Buuren, S. van
2007-01-01
The worm plot is a series of detrended Q-Q plots, split by covariate levels. The worm plot is a diagnostic tool for visualizing how well a statistical model fits the data, for finding locations at which the fit can be improved, and for comparing the fit of different models. This paper shows how
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Wickham, Hadley; Hofmann, Heike
2011-12-01
We propose a new framework for visualising tables of counts, proportions and probabilities. We call our framework product plots, alluding to the computation of area as a product of height and width, and the statistical concept of generating a joint distribution from the product of conditional and marginal distributions. The framework, with extensions, is sufficient to encompass over 20 visualisations previously described in fields of statistical graphics and infovis, including bar charts, mosaic plots, treemaps, equal area plots and fluctuation diagrams. © 2011 IEEE
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Hassan, Husain; Abeer, Ali
2014-01-01
The adenosine triphosphatase (ATP phosphohydrolase, EC 3.6.1.3.;ATPase) is a membrane -bound enzyme which transport protons across the plasma membrane using ATP as an energy source. The adenosine triphosphatase (ATPase ; EC: 3.6.1.3) was extracted from membrane preparations of adult Fasciola hepatica by chloroform treatment and purified by means of ammonium sulphate fractionation, gel filtration on sephadex G-200 and DEAE- Cellulose chromatography. The molecular weight was calculated to be 305.000 dalton by gel filtration. Kinetic experiments demonstrated a biphasic linear lineweaver - burk relationship (km=0.142 and 1.66 mM) thus revealing the existence of two substrate binding enzyme sites. In our study revealed that partial inhibition of Mg²⁺ dependent purified enzyme by oligomycin suggest the absence of mitochondrial ATPase in F. hepatica.
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Directory of Open Access Journals (Sweden)
Cheng-Yang Huang
Full Text Available Dihydropyrimidinase is a member of the cyclic amidohydrolase family, which also includes allantoinase, dihydroorotase, hydantoinase, and imidase. These metalloenzymes possess very similar active sites and may use a similar mechanism for catalysis. However, whether the substrates and inhibitors of other cyclic amidohydrolases can inhibit dihydropyrimidinase remains unclear. This study investigated the inhibition of dihydropyrimidinase by flavonoids and substrates of other cyclic amidohydrolases. Allantoin, dihydroorotate, 5-hydantoin acetic acid, acetohydroxamate, orotic acid, and 3-amino-1,2,4-triazole could slightly inhibit dihydropyrimidinase, and the IC50 values of these compounds were within the millimolar range. The inhibition of dihydropyrimidinase by flavonoids, such as myricetin, quercetin, kaempferol, galangin, dihydromyricetin, and myricitrin, was also investigated. Some of these compounds are known as inhibitors of allantoinase and dihydroorotase. Although the inhibitory effects of these flavonoids on dihydropyrimidinase were substrate-dependent, dihydromyricetin significantly inhibited dihydropyrimidinase with IC50 values of 48 and 40 μM for the substrates dihydrouracil and 5-propyl-hydantoin, respectively. The results from the Lineweaver-Burk plot indicated that dihydromyricetin was a competitive inhibitor. Results from fluorescence quenching analysis indicated that dihydromyricetin could form a stable complex with dihydropyrimidinase with the K(d value of 22.6 μM. A structural study using PatchDock showed that dihydromyricetin was docked in the active site pocket of dihydropyrimidinase, which was consistent with the findings from kinetic and fluorescence studies. This study was the first to demonstrate that naturally occurring product dihydromyricetin inhibited dihydropyrimidinase, even more than the substrate analogs (>3 orders of magnitude. These flavonols, particularly myricetin, may serve as drug leads and dirty drugs (for
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Sabiu, S; O'Neill, F H; Ashafa, A O T
2016-05-13
Corn silk (Zea mays L., Stigma maydis) is an important herb used traditionally in many parts of the world to treat array of diseases including diabetes mellitus. Inhibitors of α-amylase and α-glucosidase offer an effective strategy to modulate levels of post prandial hyperglycaemia via control of starch metabolism. This study evaluated α-amylase and α-glucosidase inhibitory potentials of corn silk aqueous extract. Active principles and antioxidant attributes of the extract were also analysed. The α-amylase inhibitory potential of the extract was investigated by reacting its different concentrations with α-amylase and starch solution, while α-glucosidase inhibition was determined by pre-incubating α-glucosidase with different concentrations of the extract followed by addition of p-nitrophenylglucopyranoside. The mode(s) of inhibition of the enzymes were determined using Lineweaver-Burke plot. In vitro analysis of the extract showed that it exhibited potent and moderate inhibitory potential against α-amylase and α-glucosidase, respectively. The inhibition was concentration-dependent with respective half-maximal inhibitory concentration (IC50) values of 5.89 and 0.93mg/mL. Phytochemical analyses revealed the presence of alkaloids, flavonoids, phenols, saponins, tannins and phytosterols as probable inhibitory constituents. Furthermore, the extract remarkably scavenges reactive oxygen species like DPPH and nitric oxide radicals, elicited good reducing power and a significant metal chelating attributes. Overall, the non-competitive and uncompetitive mechanism of action of corn silk extract is due to its inhibitory effects on α-amylase and α-glucosidase, respectively. Consequently, this will reduce the rate of starch hydrolysis, enhance palliated glucose levels, and thus, lending credence to hypoglycaemic candidature of corn silk. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.
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Directory of Open Access Journals (Sweden)
Fernanda V. A. Risso
2010-01-01
Full Text Available The aim of this work is to evaluate the effects of the butyl acetate concentration on the characteristics of free and immobilized inulinase from Kluyveromyces marxianus ATCC 16045. The mass fractions of organic solvent (OS in sodium acetate buffer (0.1 M were studied in the range from 25 to 70 %. The characteristics of both free and immobilized enzymes were not significantly affected by the OS mass fraction. The optimal temperature for the free enzyme was 55 °C at all OS mass fractions studied, whereas for the immobilized enzyme the optimum was 55 °C at 70 % of butyl acetate, and in the range from 50 to 60 °C at 25 and 50 % of OS. The optimum pH values, at all OS mass fractions, were 4.8 and 4.4 for the free and immobilized enzymes, respectively. The immobilized enzyme showed more stability at 50 °C and pH=4.8 for the whole range of OS mass fractions, since its stability was improved about 3 times. The kinetics parameters were calculated using Lineweaver-Burk plots. For the free enzyme, the vmax values were 12.5, 58.5 and 37.6 U/mL and the Km values 17.5, 280.7 and 210.4 mM at butyl acetate mass fractions of 25, 50 and 70 %, respectively. Similarly, for the immobilized enzyme, the vmax values were 38.9, 59.5 and 72.5 U/mL and the Km values 3.1, 5.4 and 14.0 mM at the same butyl acetate mass fractions, respectively.
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Li, Cailan; Xie, Jianhui; Chen, Xiaoying; Mo, Zhizhun; Wu, Wen; Liang, Yeer; Su, Zuqing; Li, Qian; Li, Yucui; Su, Ziren; Yang, Xiaobo
2016-03-01
Rhizoma Coptidis, Cortex Phellodendri, and berberine were reported to inhibit Helicobacter pylori. However, the underlying mechanism remained elusive. Urease plays a vital role in H. pylori colonization and virulence. In this work, aqueous extracts of Rhizoma Coptidis, Cortex Phellodendri of different origins, and purified berberine were investigated against H. pylori urease and jack bean urease to elucidate the inhibitory capacity, kinetics, and mechanism. Results showed that berberine was the major chemical component in Rhizoma Coptidis and Cortex Phellodendri, and the content of berberine in Rhizoma Coptidis was higher than in Cortex Phellodendri. The IC50 values of Rhizoma Coptidis were significantly lower than those Cortex Phellodendri and purified berberine, of which Coptis chinensis was shown to be the most active concentration- and time-dependent urease inhibitor. The Lineweaver-Burk plot analysis indicated that the inhibition pattern of C. chinensis against urease was noncompetitive for both H. pylori urease and jack bean urease. Thiol protectors (L-cysteine, glutathione, and dithiothreithol) significantly protected urease from the loss of enzymatic activity, while fluoride and boric acid showed weaker protection, indicating the active-site sulfhydryl group was possibly responsible for its inhibition. Furthermore, the urease inhibition proved to be reversible since C. chinensis-blocked urease could be reactivated by glutathione. The results suggested that the anti-urease activity of Rhizoma Coptidis was superior to that of Cortex Phellodendri and berberine, which was believed to be more likely to correlate to the content of total alkaloids rather than berberine monomer. The concentration- and time-dependent, reversible, and noncompetitive inhibition against urease by C. chinensis might be attributed to its interaction with the sulfhydryl group of the active site of urease. Georg Thieme Verlag KG Stuttgart · New York.
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Enantioselective inhibition of carprofen towards UDP-glucuronosyltransferase (UGT) 2B7.
Fang, Zhong-Ze; Wang, Haina; Cao, Yun-Feng; Sun, Dong-Xue; Wang, Li-Xuan; Hong, Mo; Huang, Ting; Chen, Jian-Xing; Zeng, Jia
2015-03-01
UDP-glucuronosyltransferases (UGTs)-catalyzed glucuronidation conjugation reaction plays an important role in the elimination of many important clinical drugs and endogenous substances. The present study aims to investigate the enantioselective inhibition of carprofen towards UGT isoforms. In vitro a recombinant UGT isoforms-catalyzed 4-methylumbelliferone (4-MU) glucuronidation incubation mixture was used to screen the inhibition potential of (R)-carprofen and (S)-carprofen towards multiple UGT isoforms. The results showed that (S)-carprofen exhibited stronger inhibition potential than (R)-carprofen towards UGT2B7. However, no significant difference was observed for the inhibition of (R)-carprofen and (S)-carprofen towards other UGT isoforms. Furthermore, the inhibition kinetic behavior was compared for the inhibition of (S)-carprofen and (R)-carprofen towards UGT2B7. A Lineweaver-Burk plot showed that both (S)-carprofen and (R)-carprofen exhibited competitive inhibition towards UGT2B7-catalyzed 4-MU glucuronidation. The inhibition kinetic parameter (Ki ) was calculated to be 7.0 μM and 31.1 μM for (S)-carprofen and (R)-carprofen, respectively. Based on the standard for drug-drug interaction, the threshold for (S)-carprofen and (R)-carprofen to induce a drug-drug interaction is 0.7 μM and 3.1 μM, respectively. In conclusion, enantioselective inhibition of carprofen towards UDP-glucuronosyltransferase (UGT) 2B7 was demonstrated in the present study. Using the in vitro inhibition kinetic parameter, the concentration threshold of (S)-carprofen and (R)-carprofen to possibly induce the drug-drug interaction was obtained. Therefore, clinical monitoring of the plasma concentration of (S)-carprofen is more important than (R)-carprofen to avoid a possible drug-drug interaction between carprofen and the drugs mainly undergoing UGT2B7-catalyzed metabolism. © 2014 Wiley Periodicals, Inc.
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Recurrence plots of exchange rates of currencies
Sparavigna, Amelia Carolina
2014-01-01
Used to investigate the presence of distinctive recurrent behaviours in natural processes, the recurrence plots can be applied to the analysis of economic data, and, in particular, to the characterization of exchange rates of currencies too. In this paper, we will show that these plots are able to characterize the periods of oscillation and random walk of currencies and enhance their reply to news and events, by means of texture transitions. The examples of recurrence plots given here are obt...
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137Cs profiles in erosion plots with different soil cultivation
International Nuclear Information System (INIS)
Andrello, A.C.; Appoloni, C.R.; Cassol, E.A.; Melquiades, F.L.
2006-01-01
Cesium-137 methodology has been successfully used to assess soil erosion. Seven erosion plots were sampled to determine the 137 Cs profile and to assess the erosion rates. Cesium-137 profile for native pasture plot showed an exponential decline below 5 cm depth, with little 137 Cs activity in the superficial layer (0-5 cm). Cesium-137 profile for wheat-soybean rotation plot in conventional tillage showed a uniform distribution with depth. For this plot, the soil loss occurs more in middle than upper and lower level. Cesium-137 profile for wheat-soybean rotation and wheat-maize rotation plots in no-tillage showed a similar result to the native pasture, with a minimum soil loss in the superficial layer. Cesium-137 profile for bare soil and cultivated pasture plots are similar, with a soil erosion rate of 229 t ha -1 year -1 . In the plots with a conventional tillage a greater soil loss occur in middle than upper and lower level. In no-tillage cultivation plots occurs soil loss in lower level, but no sign of soil loss neither gain in the upper level is observed. Cesium-137 methodology is a good tool to assess soil erosion and the 137 Cs profile gives a possibility to understand the soil erosion behavior in erosion plots. (author)
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Directory of Open Access Journals (Sweden)
LÚCIA HELENA AVELINO ARAUJO
2000-09-01
Full Text Available El objetivo del trabajo fue determinar los niveles de carbohidratos, proteínas solubles y aminoácidos libres de larvas, pupas hembras y adultos hembras de Catolaccus grandis (Burks (Hymenoptera: Pteromalidae criados sobre larvas del picudo del algodón envenenadas por hembras del parasitoide y por larvas de primer instar del parasitoide. Esto estudio fue conducido en la Unidade de Investigación de Control Biologico de Plagas del Departamento de Agricultura de los Estados Unidos de la América, en Weslaco, Texas. Las 20 muestras de cada uno de los tres estados de desarrollo: tercer instar larval, pupas hembra y adultos hembra del parasitoide C. grandis, fueron separadas y pesadas individualmente y se cuantió el contenido de carbohidratos totales, proteínas solubles totales y aminoácidos libres criados en diferentes sustratos. Los resultados obtenidos confirman la existencia de patrones metabólicos significativamente distintos de estos nutrientes básicos.The aim of this work was to determine the levels of carbohydrate, soluble proteins and free amino acids of larvae, pupae and adult females Catolaccus grandis Burks (Hymenoptera: Pteromalidae which were reared in cotton (Gossypium hirsutum L. r. latifolium Hutch boll weevil (Anthonomus grandis larvae venomized by ectoparasitoid of female and 1st instar ectoparasitoid larvae. This study was carried out at the Biological Control of Pests Research Unit, Weslaco, Texas. The twenty samples of each one of three stages of development: 3rd instar larval, female pupae and female adult of parasitoid C. grandis were separated and individually weighted, and levels of carbohydrates, proteins and amino acids were quantified when reared in different substrates. The results confirmed the existence of metabolic patterns significantly distinct from the basic nutrient model.
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Bhattacharya, Abhishek; Shrivastava, Ankita; Sharma, Anjana
2013-06-01
Carbonic anhydrase (CA) was purified from Micrococcus lylae and Micrococcus luteus with 49.90 and 53.8 % yield, respectively, isolated from calcium carbonate kilns. CA from M. lylae retained 80 % stability in the pH and temperature range of 6.0-8.0 and 35-45 °C, respectively. However, CA from M. luteus was stable in the pH and temperature range of 7.5-10.0 and 35-55 °C, respectively. Cross-linked enzyme aggregates (CLEAs) raised the transition temperature of M. lylae and M. luteus CA up to 67.5 and 74.0 °C, while the operational stability (T(1/20) of CA at 55 °C was calculated to be 7.7 and 12.0 h, respectively. CA from both the strains was found to be monomeric in nature with subunit molecular weight and molecular mass of 29 kDa. Ethoxozolamide was identified as the most potent inhibitor based on both IC(50) values and inhibitory constant measurement (K(i)). The K(m) and V(max) for M. lylae CA (2.31 mM; 769.23 μmol/mg/min) and M. luteus CA (2.0 mM; 1,000 μmol/mg/min) were calculated from Lineweaver-Burk plots in terms of esterase activity. Enhanced thermostability of CLEAs alleviates its role in operational stability for application at an on-site scrubber. The characteristic profile of purified CA from Micrococcus spp. advocates its effective application in biomimetic CO(2) sequestration.
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International Nuclear Information System (INIS)
Das, Devlina; Charumathi, D.; Das, Nilanjana
2011-01-01
Single and binary effects of dye Basic Violet 3 and heavy metals, 'namely', Pb(II) and Cd(II), were investigated for their role in dye and heavy metal bioaccumulation by Candida tropicalis that was grown in a sugarcane bagasse extract medium containing 8 g/L, 16 g/L or 24 g/L of sugar. The optimum pH was found to be 4.0 in the single system and 5.0 in the binary system. A central composite design was successfully used to analyse the experimental results. Four numerical correlations that were fitted to a second order quadratic equation were used to estimate optimum combinations predicted by response surface methodology. In the dye-Pb(II) binary system, C. tropicalis was capable of bioaccumulating 49.5% of the dye and 49.6% of the Pb(II), in comparison to 15.9% of the dye and 55.5% of the Cd(II) in the dye-Cd(II) binary system. In these two systems, the pollutants were dispersed at minimum working concentration levels. Competitive inhibition was observed in both the single and binary systems, which was suggested by an increase in the saturation constant, K s , and a simultaneous decrease in the specific growth rate that was calculated from Lineweaver-Burk plots. Atomic force microscopy images demonstrated changes in yeast cell morphology by exposure to these contaminants in the dye-Pb(II) binary system grown in a bioaccumulation medium.
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Directory of Open Access Journals (Sweden)
Fatma Matpan Bekler
2016-01-01
Full Text Available A thermostable and detergent-stable α-amylase from a newly isolated Anoxybacillus sp. AH1 was purifi ed and characterized. Maximum enzyme production (1874.8 U/mL was obtained at 24 h of incubation. The amylase was purified by using Sephadex G-75 gel filtration, after which an 18-fold increase in specific activity and a yield of 9 % were achieved. The molecular mass of the purified enzyme was estimated at 85 kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE. The optimum pH and temperature values of the enzyme were 7.0 and 60 °C, respectively. The enzyme was highly stable in the presence of 30 % glycerol, retaining 85 % of its original activity at 60 °C within 120 min. Km and vmax values were 0.102 μmol and 0.929 μmol/min, espectively, using Lineweaver-Burk plot. The enzyme activity was increased by various detergents, but it was significantly inhibited in the presence of urea. Mg2+ and Ca2+ also significantly activated α-amylase, while Zn2+, Cu2+ and metal ion chelators ethylenediaminetetraacetic acid (EDTA and 1,10-phenanthroline (phen greatly inhibited the enzyme activity. α-Amylase activity was enhanced by β-mercaptoethanol (β-ME and dithiothreitol (DTT to a great extent, but inhibited by p-chloromercuribenzoic acid (PCMB. Iodoacetamide (IAA and N-ethylmaleimide (NEM had a slight, whereas phenylmethylsulfonyl fluoride (PMSF had a strong inhibitory effect on the amylase activity.
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DEFF Research Database (Denmark)
Lund, Inger-Lise; Gjessing, Susanne; Hermansen, Anne-Mette
Plot 3 er første udgivelse af et alsidigt dansksystem til mellemtrinnet, hvor digitale medier er integreret i den daglige undervisning.......Plot 3 er første udgivelse af et alsidigt dansksystem til mellemtrinnet, hvor digitale medier er integreret i den daglige undervisning....
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Einmahl, J.H.J.; Gantner, M.
2009-01-01
The Half-Half (HH) plot is a new graphical method to investigate qualitatively the shape of a regression curve. The empirical HH-plot counts observations in the lower and upper quarter of a strip that moves horizontally over the scatter plot. The plot displays jumps clearly and reveals further
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Trellis plots as visual aids for analyzing split plot experiments
DEFF Research Database (Denmark)
Kulahci, Murat; Menon, Anil
2017-01-01
The analysis of split plot experiments can be challenging due to a complicated error structure resulting from restrictions on complete randomization. Similarly, standard visualization methods do not provide the insight practitioners desire to understand the data, think of explanations, generate...... hypotheses, build models, or decide on next steps. This article demonstrates the effective use of trellis plots in the preliminary data analysis for split plot experiments to address this problem. Trellis displays help to visualize multivariate data by allowing for conditioning in a general way. They can...
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9 CFR 108.2 - Plot plans, blueprints, and legends required.
2010-01-01
... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Plot plans, blueprints, and legends... REQUIREMENTS FOR LICENSED ESTABLISHMENTS § 108.2 Plot plans, blueprints, and legends required. Each applicant for an establishment license shall prepare a plot plan showing all buildings for each particular land...
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Split-Plot Designs with Mirror Image Pairs as Subplots
DEFF Research Database (Denmark)
Tyssedal, John; Kulahci, Murat; Bisgaard, Soren
2011-01-01
In this article we investigate two-level split-plot designs where the sub-plots consist of only two mirror image trials. Assuming third and higher order interactions negligible, we show that these designs divide the estimated effects into two orthogonal sub-spaces, separating sub-plot main effects...... appealing with effects of major interest free from full aliasing assuming that 3rd and higher order interactions are negligible....
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Directory of Open Access Journals (Sweden)
Mirhaghparast Seyyedeh Kimia
2015-01-01
Full Text Available In the current study, two phenoloxidases (POs from the larvae of Chilo suppressalis Walker were extracted and purified by column chromatography using Sepharyl G-100 and DEAE-Cellulose fast flow column. Two proteins possessing PO activity, named as POI and POII, were extracted by purification, 5.08- and 5.62-fold, respectively, with 8.94% and 7.31% recoveries, respectively. Also, the specific activities of POI and POII were 0.478 and 0.529 U/mg protein, respectively. Finally, the molecular weights of POI and POII were calculated as 94.6 and 95.7 kDa, respectively. Kinetic parameters of the purified phenoloxidases by Lineweaver-Burk analysis were Vmax of 2.27 and 1.11 U/mg protein and Km of 15.51 and 17.31 mM for POI and POII, respectively. Mg2+ and Cu2+ significantly increased the PO activities. Ca2+ decreased the activity of POI and showed no statistical effects on POII activity. EDTA and DTC significantly inhibited the activities of the purified enzymes, while triethylenetetramine hexaacetic acid (TTHA and RGTA showed no significant effects on enzymatic activities.
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Analysing spatially extended high-dimensional dynamics by recurrence plots
Energy Technology Data Exchange (ETDEWEB)
Marwan, Norbert, E-mail: marwan@pik-potsdam.de [Potsdam Institute for Climate Impact Research, 14412 Potsdam (Germany); Kurths, Jürgen [Potsdam Institute for Climate Impact Research, 14412 Potsdam (Germany); Humboldt Universität zu Berlin, Institut für Physik (Germany); Nizhny Novgorod State University, Department of Control Theory, Nizhny Novgorod (Russian Federation); Foerster, Saskia [GFZ German Research Centre for Geosciences, Section 1.4 Remote Sensing, Telegrafenberg, 14473 Potsdam (Germany)
2015-05-08
Recurrence plot based measures of complexity are capable tools for characterizing complex dynamics. In this letter we show the potential of selected recurrence plot measures for the investigation of even high-dimensional dynamics. We apply this method on spatially extended chaos, such as derived from the Lorenz96 model and show that the recurrence plot based measures can qualitatively characterize typical dynamical properties such as chaotic or periodic dynamics. Moreover, we demonstrate its power by analysing satellite image time series of vegetation cover with contrasting dynamics as a spatially extended and potentially high-dimensional example from the real world. - Highlights: • We use recurrence plots for analysing partially extended dynamics. • We investigate the high-dimensional chaos of the Lorenz96 model. • The approach distinguishes different spatio-temporal dynamics. • We use the method for studying vegetation cover time series.
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Einmahl, J.H.J.; Gantner, M.
2012-01-01
The Half-Half (HH) plot is a new graphical method to investigate qualitatively the shape of a regression curve. The empirical HH-plot counts observations in the lower and upper quarter of a strip that moves horizontally over the scatterplot. The plot displays jumps clearly and reveals further
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Plasma physics plotting package
International Nuclear Information System (INIS)
Hyman, D.H.
1981-02-01
We describe a package of plotting routines that do up to six two- or three-dimensional plots on a frame with minimal loss of resolution. The package now runs on a PDP-10 with PLOT-10 TCS primitives and on a Control Data Corporation-7600 and a Cray-1 with TV80LIB primitives on the National Magnetic Fusion Energy Computer Center network. The package is portable to other graphics systems because only the primitive plot calls are used from the underlying system's graphics package
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MatrixPlot: visualizing sequence constraints
DEFF Research Database (Denmark)
Gorodkin, Jan; Stærfeldt, Hans Henrik; Lund, Ole
1999-01-01
MatrixPlot: visualizing sequence constraints. Sub-title Abstract Summary : MatrixPlot is a program for making high-quality matrix plots, such as mutual information plots of sequence alignments and distance matrices of sequences with known three-dimensional coordinates. The user can add information...
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DEFF Research Database (Denmark)
Jensen, Lotte Groth; Bossen, Claus
2016-01-01
different socio-technical systems (paper-based and electronic patient records). Drawing on the theory of distributed cognition and narrative theory, primarily inspired by the work done within health care by Cheryl Mattingly, we propose that the creation of overview may be conceptualised as ‘distributed plot......-making’. Distributed cognition focuses on the role of artefacts, humans and their interaction in information processing, while narrative theory focuses on how humans create narratives through the plot construction. Hence, the concept of distributed plot-making highlights the distribution of information processing...
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MMS control system analysis using automated root-locus plot generation
International Nuclear Information System (INIS)
Hefler, J.W.
1987-01-01
Use of the Modular Modeling System (MMS) for control systems improvement has been impeded by the need to plot eigenvalues manually. This problem has been solved by an automatic eigenvalue plotting routine. A practical procedure for control systems analysis based upon automatically generated root-locus plots has been developed using the Advanced Continuous Simulation Language (ACSL)-based version of the Modular Modeling System. Examples are given of typical ACSL run-time statements. Actual root-locus and time history plots are shown for simple models (4 state variables). More complex models are discussed. The plots show the control systems response before and after the determination of tuning parameters using the methods described
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Hollmann, J; Niemann, R; Buddecke, E
1986-01-01
A 3'-phosphoadenylylsulfate:chondroitin sulfotransferase (EC 2.8.2.5) was purified to homogeneity (about 760-fold) from the cytosolic fraction of calf arterial tissue by Con A-Sepharose, ion exchange and affinity chromatography. The enzyme has a molecular mass of 38000 Da, optimal activity at pH 6.0 (100%) and 7.25 (75%), requires divalent cations for maximal activity (Mn2+ greater than Mg2+, Ca2+) and exhibits specificity towards desulfated chondroitin sulfate and oligosaccharides derived therefrom. The enzyme transfers sulfate groups from [35S]phosphoadenylylsulfate exclusively to C-6 OH groups of N-acetylgalactosamine units of the acceptor substrates. Maximal sulfate transfer occurs at 2mM chondroitin disaccharide units (100%), the transfer rates decreasing with decreasing chain length in the order deca (55%), octa (17%) and hexasaccharides (4%). Lineweaver-Burk plots revealed equal maximal velocities for chondroitin, deca-, octa- and hexasaccharide, but decreasing Km values. Chondroitin 4-sulfate has 21% of the acceptor potency exhibited by chondroitin, whereas dermatan sulfate, heparan sulfate and hyaluronate and the chondroitin tetrasaccharide showed no acceptor properties. Analysis of the reaction products formed by prolonged enzymatic sulfation of a reduced chondroitin hexasaccharide [GlcA-GalNAc]2-GlcA-GalNAc-ol revealed that the preterminal N-acetylgalactosamine from the non-reducing end and the internal N-acetylgalactosamine but not the N-acetylgalactosaminitol were sulfated and that no hexasaccharide disulfate was formed by the action of chondroitin 6-sulfotransferase. Chondroitin 6-sulfotransferase is considered to possess a binding region capable of accommodating a nonsulfated oligosaccharide sequence of at least six sugars and is believed to act in the course of chondroitin sulfate synthesis in cooperation with, but shortly after, the enzymes involved in the chain elongation reaction.
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iCanPlot: visual exploration of high-throughput omics data using interactive Canvas plotting.
Directory of Open Access Journals (Sweden)
Amit U Sinha
Full Text Available Increasing use of high throughput genomic scale assays requires effective visualization and analysis techniques to facilitate data interpretation. Moreover, existing tools often require programming skills, which discourages bench scientists from examining their own data. We have created iCanPlot, a compelling platform for visual data exploration based on the latest technologies. Using the recently adopted HTML5 Canvas element, we have developed a highly interactive tool to visualize tabular data and identify interesting patterns in an intuitive fashion without the need of any specialized computing skills. A module for geneset overlap analysis has been implemented on the Google App Engine platform: when the user selects a region of interest in the plot, the genes in the region are analyzed on the fly. The visualization and analysis are amalgamated for a seamless experience. Further, users can easily upload their data for analysis--which also makes it simple to share the analysis with collaborators. We illustrate the power of iCanPlot by showing an example of how it can be used to interpret histone modifications in the context of gene expression.
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DEFF Research Database (Denmark)
Kampf, Constance
summarizes Burke's essay, "Antinomies of Definition," from a Grammar of Motives as demonstrating that 'the intrinsic and the extrinsic can change places' "such that to define is always to contextualize and to uncover the absence of a solid ground for a claim to knowledge."2 So if the intrinsic...... allow for user agency, whereas entitlement appears to be focused more at the cultural level, with the agency shared by the 'tribe,' the language itself, and the context of situation.1 In other words, "terministic screens" appear to be extrinsic, while "entitlement" appears to be intrinsic. Carmichael...... and extrinsic can change places, the interpretive process of "terministic screens" and the constitutive process of "entitlement" may well be reciprocal. The implications of this reciprocity can be used as a basis for understanding the linguistic and cultural components of Corporate Social Responsibility...
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A Burke-Schumann Analysis of Dual-Flame Structure Supported by a Burning Droplet
Nayagam, V.; Dietrich, D.; Williams, F. A.
2016-01-01
Droplet combustion experiments carried out onboard the International Space Station (ISS), using pure fuels and fuel mixtures, have shown that quasi-steady burning can be sustained by a non-traditional flame configuration, namely a "cool flame" burning in the "partial-burning" regime where both fuel and oxygen leak through the low-temperature controlled flame-sheet. Recent experiments involving large, bi-component fuel (n-decane and hexanol, 50/50 by volume) droplets at elevated pressures show that the visible, hot flame becomes extremely weak while the burning rate remains relatively high, suggesting the possibility of simultaneous presence of "cool" and "hot" flames of roughly equal importance. The radiant output from these bi-component droplets is relatively high and cannot be accounted for only by the presence of a visible hot-flame. In this analysis we explore the theoretical possibility of a dual-flame structure, where one flame lies close to the droplet surface called the "cool-flame," and other farther away from the droplet surface, termed the "hot-flame." A Burke-Schumann analysis of this dual-structure seems to indicate such flame structures are possible over a narrow range of initial conditions. Theoretical results can be compared against available experimental data for pure and bi-component fuel droplet combustion to test how realistic the model may be.
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Intelligence Constraints on Terrorist Network Plots
Woo, Gordon
Since 9/11, the western intelligence and law enforcement services have managed to interdict the great majority of planned attacks against their home countries. Network analysis shows that there are important intelligence constraints on the number and complexity of terrorist plots. If two many terrorists are involved in plots at a given time, a tipping point is reached whereby it becomes progressively easier for the dots to be joined and for the conspirators to be arrested, and for the aggregate evidence to secure convictions. Implications of this analysis are presented for the campaign to win hearts and minds.
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A drawback and an improvement of the classical Weibull probability plot
International Nuclear Information System (INIS)
Jiang, R.
2014-01-01
The classical Weibull Probability Paper (WPP) plot has been widely used to identify a model for fitting a given dataset. It is based on a match between the WPP plots of the model and data in shape. This paper carries out an analysis for the Weibull transformations that create the WPP plot and shows that the shape of the WPP plot of the data randomly generated from a distribution model can be significantly different from the shape of the WPP plot of the model due to the high non-linearity of the Weibull transformations. As such, choosing model based on the shape of the WPP plot of data can be unreliable. A cdf-based weighted least squares method is proposed to improve the parameter estimation accuracy; and an improved WPP plot is suggested to avoid the drawback of the classical WPP plot. The appropriateness and usefulness of the proposed estimation method and probability plot are illustrated by simulation and real-world examples
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2014-01-01
Background Urease, one of the highly efficient known enzymes, catalyzes the hydrolysis of urea into ammonia and carbon dioxide. The present study aimed to extract urease from pea seeds (Pisum Sativum L). The enzyme was then purified in three consequence steps: acetone precipitation, DEAE-cellulose ion-exchange chromatography, and gel filtration chromatography (Sephacryl S-200 column). Results The purification fold was 12.85 with a yield of 40%. The molecular weight of the isolated urease was estimated by chromatography to be 269,000 Daltons. Maximum urease activity (190 U/g) was achieved at the optimum conditions of 40°C and pH of 7.5 after 5 min of incubation. The kinetic parameters, K m and V max , were estimated by Lineweaver-Burk fits and found to be 500 mM and 333.3 U/g, respectively. The thermodynamic constants of activation, ΔH, E a , and ΔS, were determined using Arrhenius plot and found to be 21.20 kJ/mol, 23.7 kJ/mol, and 1.18 kJ/mol/K, respectively. Conclusions Urease was purified from germinating Pisum Sativum L. seeds. The purification fold, yield, and molecular weight were determined. The effects of pH, concentration of enzyme, temperature, concentration of substrate, and storage period on urease activity were examined. This may provide an insight on the various aspects of the property of the enzyme. The significance of extracting urease from different sources could play a good role in understanding the metabolism of urea in plants. PMID:25065975
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The kinetics of phagocytosis of 198Au colloids ''in vitro''
International Nuclear Information System (INIS)
Astorri, N.L.; Bergoc, R.M.; Bianchin, A.M.; Caro, R.A.; Ihlo, J.E.; Rivera, E.S.
1982-01-01
The kinetics of the phagocytosis of 198-Au colloids by macrophages ''in vitro'' was studied by incubating during 5 hours phagocytic cells from the liver and the spleen of Wistar rats with colloidal radiogold particles, in the presence of an adequate culture medium (TC-199 with 10 per cent of Bovine Fetal Serum). In each experiment, the number of colloidal gold particles offered to each phatocytic cell, (Au) 0 and the mean rate of phagocytosis v, were calculated. The latter value was determined by measuring the radioactivity incorporated into the phagocytic cells during the incubation; it was expressed as the number of phagocytized colloidal gold particles per cell per minute. The values of log v = f [log (Au) 0 ] were plotted. The Lineweaver-Burk analysis of the results demonstrates that the kinetics of the phagocytosis of colloidal radiogold particles ''in vitro'' follows a model similar to Michaelis-Menten equations for enzyme reactions. The values of the substratum constant Ks and maximun velocity Vm were obtained by the regression analysis of the 1/v vs. 1/(Au) 0 graph. Vm was equal to 9.44 x 10 and 1.63 x 10 phagocytized colloidal gold particles per cell per minute for liver and spleen macrophages, respectively. Ks was equal to 6.01 x 10 9 and 8.02 x 10 8 colloidal gold particles per cell for liver and spleen macrophages, respectively. The significance of these differences is discussed and attributed mainly to a change of the specific engulfment rate constant. (author) [es
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International Nuclear Information System (INIS)
Kamal, L.A.; Le Quan-Bui, K.H.; Meyer, P.
1984-01-01
The uptake and content of serotonin in blood platelets were studied in patients with essential hypertension and in five families in which at least one member was hypertensive. Blood was obtained from male and female normotensive volunteers and hypertensive patients who were free of medication. Lineweaver-Burk plots of 3H-serotonin uptake from both control subjects and hypertensive patients were linear, which suggested simple Michaelis-Menten uptake kinetics. The maximal uptake velocity (Vmax) in hypertensive patients was significantly lower than in control subjects (control . 41.7 +/- 3.3 pmol/min/10(8) platelets, n . 17; hypertensive . 26.6 +/- 3.0 pmol/min/10(8) platelets, n . 16; p less than 0.005). The affinity constant (Km) was slightly but significantly lower in hypertensive patients (control . 0.70 +/- 0.08 microM; hypertensive . 0.46 +/- 0.08 microM; p less than 0.05). The serotonin content in blood platelets determined by high pressure liquid chromatography with electrochemical detection was significantly lower in hypertensive patients (control . 165.0 +/- 12.9 nmol/10(11) platelets, n . 29; hypertensive . 105.9 +/- 10.4 nmol/10(11) platelets, n . 27; p less than 0.001). In the five families investigated, the lowered serotonin content was observed in some normotensive members. The reduced number of carriers of serotonin uptake and the slight decrease in the affinity constant observed in platelets of patients with essential hypertension suggest that serotonin metabolism is altered in essential hypertension and that blood platelets may be a useful model in studying the serotonergic modifications at the molecular level
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Splatterplots: overcoming overdraw in scatter plots.
Mayorga, Adrian; Gleicher, Michael
2013-09-01
We introduce Splatterplots, a novel presentation of scattered data that enables visualizations that scale beyond standard scatter plots. Traditional scatter plots suffer from overdraw (overlapping glyphs) as the number of points per unit area increases. Overdraw obscures outliers, hides data distributions, and makes the relationship among subgroups of the data difficult to discern. To address these issues, Splatterplots abstract away information such that the density of data shown in any unit of screen space is bounded, while allowing continuous zoom to reveal abstracted details. Abstraction automatically groups dense data points into contours and samples remaining points. We combine techniques for abstraction with perceptually based color blending to reveal the relationship between data subgroups. The resulting visualizations represent the dense regions of each subgroup of the data set as smooth closed shapes and show representative outliers explicitly. We present techniques that leverage the GPU for Splatterplot computation and rendering, enabling interaction with massive data sets. We show how Splatterplots can be an effective alternative to traditional methods of displaying scatter data communicating data trends, outliers, and data set relationships much like traditional scatter plots, but scaling to data sets of higher density and up to millions of points on the screen.
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Model-independent plot of dynamic PET data facilitates data interpretation and model selection.
Munk, Ole Lajord
2012-02-21
When testing new PET radiotracers or new applications of existing tracers, the blood-tissue exchange and the metabolism need to be examined. However, conventional plots of measured time-activity curves from dynamic PET do not reveal the inherent kinetic information. A novel model-independent volume-influx plot (vi-plot) was developed and validated. The new vi-plot shows the time course of the instantaneous distribution volume and the instantaneous influx rate. The vi-plot visualises physiological information that facilitates model selection and it reveals when a quasi-steady state is reached, which is a prerequisite for the use of the graphical analyses by Logan and Gjedde-Patlak. Both axes of the vi-plot have direct physiological interpretation, and the plot shows kinetic parameter in close agreement with estimates obtained by non-linear kinetic modelling. The vi-plot is equally useful for analyses of PET data based on a plasma input function or a reference region input function. The vi-plot is a model-independent and informative plot for data exploration that facilitates the selection of an appropriate method for data analysis. Copyright © 2011 Elsevier Ltd. All rights reserved.
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Density Distribution Sunflower Plots
Directory of Open Access Journals (Sweden)
William D. Dupont
2003-01-01
Full Text Available Density distribution sunflower plots are used to display high-density bivariate data. They are useful for data where a conventional scatter plot is difficult to read due to overstriking of the plot symbol. The x-y plane is subdivided into a lattice of regular hexagonal bins of width w specified by the user. The user also specifies the values of l, d, and k that affect the plot as follows. Individual observations are plotted when there are less than l observations per bin as in a conventional scatter plot. Each bin with from l to d observations contains a light sunflower. Other bins contain a dark sunflower. In a light sunflower each petal represents one observation. In a dark sunflower, each petal represents k observations. (A dark sunflower with p petals represents between /2-pk k and /2+pk k observations. The user can control the sizes and colors of the sunflowers. By selecting appropriate colors and sizes for the light and dark sunflowers, plots can be obtained that give both the overall sense of the data density distribution as well as the number of data points in any given region. The use of this graphic is illustrated with data from the Framingham Heart Study. A documented Stata program, called sunflower, is available to draw these graphs. It can be downloaded from the Statistical Software Components archive at http://ideas.repec.org/c/boc/bocode/s430201.html . (Journal of Statistical Software 2003; 8 (3: 1-5. Posted at http://www.jstatsoft.org/index.php?vol=8 .
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DEFF Research Database (Denmark)
Svendsen, Anders Jørgen; Holmskov, U; Bro, Peter
1995-01-01
and systemic lupus erythematosus from another previously published study (Macanovic, M. and Lachmann, P.J. (1979) Clin. Exp. Immunol. 38, 274) are also represented using ratio plots. Our observations indicate that analysis by regression analysis may often be misleading....... hitherto unnoted differences between controls and patients with either rheumatoid arthritis or systemic lupus erythematosus. For this we use simple, but unconventional, graphic representations of the data, based on difference plots and ratio plots. Differences between patients with Burkitt's lymphoma...
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Isodose plotting for pen plotters
International Nuclear Information System (INIS)
Rosen, I.I.
1985-01-01
A general algorithm for treatment plan isodose line plotting is described which is particularly useful for pen plotters. Unlike other methods of plotting isodose lines, this algorithm is designed specifically to reduce pen motion, thereby reducing plotting time and wear on the transport mechanism. Points with the desired dose value are extracted from the dose matrix and stored, sorted into continuous contours, and then plotted. This algorithm has been implemented on DEC PDP-11/60 and VAX-11/780 computers for use with two models of Houston Instrument pen plotters, two models of Tektronix vector graphics terminals, a DEC VT125 raster graphics terminal, and a DEC VS11 color raster graphics terminal. Its execution time is similar to simpler direct-plotting methods
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Directory of Open Access Journals (Sweden)
Khatri K
2015-07-01
Full Text Available Objective: To report functional outcome in Posterior Cruciate Ligament (PCL tibial avulsion fractures treated with open reduction and internal fixation through Burks and Schaffer approach. The patient specific functional outcome measures like IKDC grading together with objective grading with stress radiographs have rarely been used to assess PCL tibial avulsion fractures. Material and Methods: Twenty seven patients (21 males and 6 females were included in the study. The mean follow up duration was 22.30±6.82 months. They were assessed using international knee documentation committee (IKDC grades, Lysholm scoring and stress radiography. The injury severity scores (ISS of the patients were also recorded. Results: The mean Lysholm scores at the time of last follow up was 90.85±5.58. The IKDC grades achieved were normal in 20 patients, near normal in five and abnormal in two. The PCL laxity determined on active hamstring contraction stress radiography was grade I in 20 cases and grade II in seven cases. All patients had achieved bony union of tibial avulsion fractures at the time of last follow up. Statistically significant association was found between higher ISS and lower Lysholm scores. (t=3.455, p=0.0019. Good IKDC grades were associated with higher Lysholm scores (analysis of variance, F=32.51, p<.0001.There was no correlation between PCL laxity and functional outcome (t=.857, p =0.399. Conclusion: PCL tibial avulsion fractures treated through Burk and Schaffer approach with open reduction and internal fixation produces good results. The early rehabilitation without cast immobilisation prevents arthrofibrosis.
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How much information is contained in a recurrence plot?
International Nuclear Information System (INIS)
Thiel, Marco; Romano, M. Carmen; Kurths, Juergen
2004-01-01
Recurrence plots have recently been recognized as a powerful tool for the analysis of data. Not only the visualization of structures of the time series but also the possibility to estimate invariants from them and the possibility to analyze non-stationary data sets are remarkable. However, the question of how much information is encoded in such a two-dimensional and binary representation has not been discussed so far. In this Letter we show that - under some conditions - it is possible to reconstruct an attractor from the recurrence plot, at least topologically. This means that all relevant dynamical information is contained in the plot
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Cysteine Protease (Capparin from Capsules of Caper (Capparis spinosa
Directory of Open Access Journals (Sweden)
Yasar Demir
2008-01-01
Full Text Available Proteases are enzymes that perform very important functions in organisms and are used for a variety of objectives in vitro. In recent years, proteases have been used for clinical, pharmaceutical (alimentary digestion, anti-inflammatory, etc. and industrial applications (cheese production, meat tenderizing, leather tanning. In this research, a protease has been purified from capsules of caper (Capparis spinosa and characterized. Caper plants have been used for food and medicine since ancient times. The plant grows abundantly in certain regions of Turkey. Ammonium sulphate fractionation and a CM Sephadex column were used for purification of the enzyme. The purification enzyme has an optimum pH=5.0 and its optimum temperature was 60 °C. The vmax and Km values determined by Lineweaver-Burk graphics were 1.38 μg/(L·min and 0.88 μg/L, respectively. The purification degree and the molecular mass of the enzyme (46 kDa were determined by SDS-PAGE and gel filtration chromatography. It was investigated whether the purified and characterized protease could cause milk to congeal or digest chicken and cow meat. The results show that protease can be used for industrial production.
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[Comparative quality measurements part 3: funnel plots].
Kottner, Jan; Lahmann, Nils
2014-02-01
Comparative quality measurements between organisations or institutions are common. Quality measures need to be standardised and risk adjusted. Random error must also be taken adequately into account. Rankings without consideration of the precision lead to flawed interpretations and enhances "gaming". Application of confidence intervals is one possibility to take chance variation into account. Funnel plots are modified control charts based on Statistical Process Control (SPC) theory. The quality measures are plotted against their sample size. Warning and control limits that are 2 or 3 standard deviations from the center line are added. With increasing group size the precision increases and so the control limits are forming a funnel. Data points within the control limits are considered to show common cause variation; data points outside special cause variation without the focus of spurious rankings. Funnel plots offer data based information about how to evaluate institutional performance within quality management contexts.
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Plotting system for the MINCS code
International Nuclear Information System (INIS)
Watanabe, Tadashi
1990-08-01
The plotting system for the MINCS code is described. The transient two-phase flow analysis code MINCS has been developed to provide a computational tool for analysing various two-phase flow phenomena in one-dimensional ducts. Two plotting systems, namely the SPLPLOT system and the SDPLOT system, can be used as the plotting functions. The SPLPLOT system is used for plotting time transients of variables, while the SDPLOT system is for spatial distributions. The SPLPLOT system is based on the SPLPACK system, which is used as a general tool for plotting results of transient analysis codes or experiments. The SDPLOT is based on the GPLP program, which is also regarded as one of the general plotting programs. In the SPLPLOT and the SDPLOT systems, the standardized data format called the SPL format is used in reading data to be plotted. The output data format of MINCS is translated into the SPL format by using the conversion system called the MINTOSPL system. In this report, how to use the plotting functions is described. (author)
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IPLOT, interactive MELCOR data plotting system
International Nuclear Information System (INIS)
2008-01-01
1 - Description of program or function: IPLOT is an interactive MELCOR data plotting system. It provides several kinds of GUI interfaces for a flexible data plotting. IPLOT capabilities include creation, saving and loading of user specified MELCOR variables trend graphs. IPLOT can use one or several plot files for a graph generation while the graphs can be either in one window or in several windows. Besides IPLOT provides several graph convenient functions such as zooming, re-sizing, printing for a detail analysis of severe accidents. 2 - Methods: Trend values seeking in a plot file is performed by a binary search method for fast performance. 3 - Restrictions on the complexity of the problem: MELCOR plot files are required for plotting
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Saugel, Bernd; Grothe, Oliver; Wagner, Julia Y
2015-08-01
When comparing 2 technologies for measuring hemodynamic parameters with regard to their ability to track changes, 2 graphical tools are omnipresent in the literature: the 4-quadrant plot and the polar plot recently proposed by Critchley et al. The polar plot is thought to be the more advanced statistical tool, but care should be taken when it comes to its interpretation. The polar plot excludes possibly important measurements from the data. The polar plot transforms the data nonlinearily, which may prevent it from being seen clearly. In this article, we compare the 4-quadrant and the polar plot in detail and thoroughly describe advantages and limitations of each. We also discuss pitfalls concerning the methods to prepare the researcher for the sound use of both methods. Finally, we briefly revisit the Bland-Altman plot for the use in this context.
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Normal probability plots with confidence.
Chantarangsi, Wanpen; Liu, Wei; Bretz, Frank; Kiatsupaibul, Seksan; Hayter, Anthony J; Wan, Fang
2015-01-01
Normal probability plots are widely used as a statistical tool for assessing whether an observed simple random sample is drawn from a normally distributed population. The users, however, have to judge subjectively, if no objective rule is provided, whether the plotted points fall close to a straight line. In this paper, we focus on how a normal probability plot can be augmented by intervals for all the points so that, if the population distribution is normal, then all the points should fall into the corresponding intervals simultaneously with probability 1-α. These simultaneous 1-α probability intervals provide therefore an objective mean to judge whether the plotted points fall close to the straight line: the plotted points fall close to the straight line if and only if all the points fall into the corresponding intervals. The powers of several normal probability plot based (graphical) tests and the most popular nongraphical Anderson-Darling and Shapiro-Wilk tests are compared by simulation. Based on this comparison, recommendations are given in Section 3 on which graphical tests should be used in what circumstances. An example is provided to illustrate the methods. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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Mechanism of antihypertensive effect of Mucuna pruriens L. seed extract and its isolated compounds.
Khan, Mohammad Yaseen; Kumar, Vimal
2017-06-21
Background In the search of safe and effective lead molecules from natural sources, Mucuna pruriens (MP) L. (Fabaceae) seeds were utilized for exploring the antihypertensive potential. Traditionally, it is used as diuretic and hypotensive. Methods Bioassay-guided fractions were utilized for the isolation of active compounds by column chromatography. IC50 value, enzyme kinetics and inhibition mechanism were determined. In vivo time and dose-dependent hypotensive study followed by changes in mean arterial pressure (MAP) induced by angiotensin I (3 nmol/kg), angiotensin II (3 nmol/kg), and bradykinin (10 nmol/kg) in anesthetized rats was done. Plasma and tissue angiotensin I-converting enzyme (ACE) activities were also determined. Results Phytochemical analysis by spectroscopic techniques revealed the presence of known compounds like genistein, ursolic acid and L-DOPA from the ethyl acetate and water fraction, respectively. In vitro study revealed MP ethyl acetate (MPEA) fraction and genistein as the most active fraction (IC50 156.45 µg/mL) and compound (IC50 253.81 µM), respectively. Lineweaver-Burk plots revealed a non-competitive mode of inhibition. ACE protein precipitation was the suggested mechanism for inhibition. The extract showed a time- and dose-dependent decrease in MAP. Genistein was able to dose-dependently reduce the MAP, up to 53±1.5 mmHg (40 mg/kg, i.v.). As compared to control, it showed a dose-dependent decrease in plasma ACE activity of 40.61 % and 54.76 % at 10 mg/kg and 20 mg/kg, respectively. It also decreased the ACE activity in the aorta (107.67nM/ml min at 10 mg, p<0.001; 95.33nM/ml min at 20 mg p<0.001). Captopril was used as a standard for various in vitro and in vivo assays. Conclusions The study revealed the antihypertensive potential of MP seed compounds via ACE inhibition.
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Generalized Probability-Probability Plots
Mushkudiani, N.A.; Einmahl, J.H.J.
2004-01-01
We introduce generalized Probability-Probability (P-P) plots in order to study the one-sample goodness-of-fit problem and the two-sample problem, for real valued data.These plots, that are constructed by indexing with the class of closed intervals, globally preserve the properties of classical P-P
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In vitro measurement of avidity of radioiodinated antibodies
International Nuclear Information System (INIS)
Badger, C.C.; Krohn, K.A.; Bernstein, I.D.
1987-01-01
A determination of the ability of radiolabeled antibodies to bind to their target antigen is an essential step in the initial selection of antibodies for clinical use as well as a quality control measure. In our studies of the 131 I-labeled anti-Thy 1.1 antibody treatment of murine lymphoma, we have used cell binding assays with a combination of Lineweaver-Burk analysis to determine immunoreactivity and Scatchard analysis to determine antibody avidity. Both assays were systematically influenced by target cell fixation and measurement of avidity was dependent on immunoreactivity. For 131 I-labeled anti-Thy 1.1 antibody, avidity was a much more sensitive indicator of iodination damage and predictor of in vivo behavior than was immunoreactivity, while for other antibodies immunoreactivity has been a better indicator of labeling damage. Thus, immunoreactivity and avidity assays are complementary and knowledge of both factors is required for the design of sensitive quality control procedures for radiolabeled antibodies. (author)
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In vitro inhibition of choline acetyltransferase by a series of 2-benzylidene-3-quinuclidinones
International Nuclear Information System (INIS)
Capacio, B.R.
1988-01-01
Ten substituted 2-benzylidene-3-quinuclidinones were synthesized and evaluated for their relative potency as in vitro inhibitors of choline acetyltransferase (ChAT). Acetylcholine (ACh) synthesis was followed radiometrically by the incorporation of labeled acetate originating from 14 C-acetyl-CoA. Woolf-Augustinsson-Hofstee data analysis was used to calculate Vmax, Km, and Ki values. The inhibition was found to be noncompetitive or uncompetitive with respect to choline. Quantitative structure activity relationship correlations demonstrated a primary dependence on κ-σ, as well as steric properties of the substituted benzene ring. Additional radiometric and spectrophotometric were performed with 2-(3'-methyl)-benzylidene-3-quinuclidinone, one of the more potent analogs, to further elucidate the inhibitory mechanism. ChAT-mediated cleavage of ACh was measured spectrophotometrically by following the appearance of NADH at 340 nanometers in an enzyme coupled assay. Lineweaver-Burk analysis indicated mixed or uncompetitive inhibition with respect to both substrates of the forward reaction, suggesting interference with a rate limiting step
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Phenols removal by immobilized tyrosinase reactor in on-line high performance liquid chromatography
International Nuclear Information System (INIS)
Girelli, Anna Maria; Mattei, Enrico; Messina, Antonella
2006-01-01
The development of an immobilized enzyme reactor (IMER) based on tyrosinase immobilized on aminopropyl-controlled pore glass (AP-CPG) for the removal of phenols from model aqueous solutions was reported. To elucidate the influence of the substrate nature, the apparent (V ' max , K ' m ) and the inherent (V max , K m ) Michaelis-Menten constants were determined by Lineweaver-Burk method and the external diffusional contributions on measured enzyme activities were removed by a graphical method. The dephenolization process was realized by recycling the phenol solutions through the bioreactor connected to a chitosan trap in order to remove the colored quinone-type products of the tyrosinase reactions. The results indicated that a complete removal of phenol derivatives in the range of 150-300min, with the exception of 60% removal for phenol reached in 400min, was obtained. The observed sequence: cresol>4-methylcathecol>catechol>4-Cl-phenol-bar phenol was in accordance to the V ' max /K ' m values
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Checking the Adequacy of Fit of Models from Split-Plot Designs
DEFF Research Database (Denmark)
Almini, A. A.; Kulahci, Murat; Montgomery, D. C.
2009-01-01
models. In this article, we propose the computation of two R-2, R-2-adjusted, prediction error sums of squares (PRESS), and R-2-prediction statistics to measure the adequacy of fit for the WP and the SP submodels in a split-plot design. This is complemented with the graphical analysis of the two types......One of the main features that distinguish split-plot experiments from other experiments is that they involve two types of experimental errors: the whole-plot (WP) error and the subplot (SP) error. Taking this into consideration is very important when computing measures of adequacy of fit for split-plot...... of errors to check for any violation of the underlying assumptions and the adequacy of fit of split-plot models. Using examples, we show how computing two measures of model adequacy of fit for each split-plot design model is appropriate and useful as they reveal whether the correct WP and SP effects have...
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Standardized mean differences cause funnel plot distortion in publication bias assessments.
Zwetsloot, Peter-Paul; Van Der Naald, Mira; Sena, Emily S; Howells, David W; IntHout, Joanna; De Groot, Joris Ah; Chamuleau, Steven Aj; MacLeod, Malcolm R; Wever, Kimberley E
2017-09-08
Meta-analyses are increasingly used for synthesis of evidence from biomedical research, and often include an assessment of publication bias based on visual or analytical detection of asymmetry in funnel plots. We studied the influence of different normalisation approaches, sample size and intervention effects on funnel plot asymmetry, using empirical datasets and illustrative simulations. We found that funnel plots of the Standardized Mean Difference (SMD) plotted against the standard error (SE) are susceptible to distortion, leading to overestimation of the existence and extent of publication bias. Distortion was more severe when the primary studies had a small sample size and when an intervention effect was present. We show that using the Normalised Mean Difference measure as effect size (when possible), or plotting the SMD against a sample size-based precision estimate, are more reliable alternatives. We conclude that funnel plots using the SMD in combination with the SE are unsuitable for publication bias assessments and can lead to false-positive results.
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Atrial fibrillation detection by heart rate variability in Poincare plot.
Park, Jinho; Lee, Sangwook; Jeon, Moongu
2009-12-11
Atrial fibrillation (AFib) is one of the prominent causes of stroke, and its risk increases with age. We need to detect AFib correctly as early as possible to avoid medical disaster because it is likely to proceed into a more serious form in short time. If we can make a portable AFib monitoring system, it will be helpful to many old people because we cannot predict when a patient will have a spasm of AFib. We analyzed heart beat variability from inter-beat intervals obtained by a wavelet-based detector. We made a Poincare plot using the inter-beat intervals. By analyzing the plot, we extracted three feature measures characterizing AFib and non-AFib: the number of clusters, mean stepping increment of inter-beat intervals, and dispersion of the points around a diagonal line in the plot. We divided distribution of the number of clusters into two and calculated mean value of the lower part by k-means clustering method. We classified data whose number of clusters is more than one and less than this mean value as non-AFib data. In the other case, we tried to discriminate AFib from non-AFib using support vector machine with the other feature measures: the mean stepping increment and dispersion of the points in the Poincare plot. We found that Poincare plot from non-AFib data showed some pattern, while the plot from AFib data showed irregularly irregular shape. In case of non-AFib data, the definite pattern in the plot manifested itself with some limited number of clusters or closely packed one cluster. In case of AFib data, the number of clusters in the plot was one or too many. We evaluated the accuracy using leave-one-out cross-validation. Mean sensitivity and mean specificity were 91.4% and 92.9% respectively. Because pulse beats of ventricles are less likely to be influenced by baseline wandering and noise, we used the inter-beat intervals to diagnose AFib. We visually displayed regularity of the inter-beat intervals by way of Poincare plot. We tried to design an
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Iron status determination in pregnancy using the Thomas plot.
Weyers, R; Coetzee, M J; Nel, M
2016-04-01
Physiological changes during pregnancy affect routine tests for iron deficiency. The reticulocyte haemoglobin equivalent (RET-He) and serum-soluble transferrin receptor (sTfR) assay are newer diagnostic parameters for the detection of iron deficiency, combined in the Thomas diagnostic plot. We used this plot to determine the iron status of pregnant women presenting for their first visit to an antenatal clinic in Bloemfontein, South Africa. Routine laboratory tests (serum ferritin, full blood count and C-reactive protein) and RET-He and sTfR were performed. The iron status was determined using the Thomas plot. For this study, 103 pregnant women were recruited. According to the Thomas plot, 72.8% of the participants had normal iron stores and erythropoiesis. Iron-deficient erythropoiesis was detected in 12.6%. A third of participants were anaemic. Serum ferritin showed excellent sensitivity but poor specificity for detecting depleted iron stores. HIV status had no influence on the iron status of the participants. Our findings reiterate that causes other than iron deficiency should be considered in anaemic individuals. When compared with the Thomas plot, a low serum ferritin is a sensitive but nonspecific indicator of iron deficiency. The Thomas plot may provide useful information to identify pregnant individuals in whom haematologic parameters indicate limited iron availability for erythropoiesis. © 2015 John Wiley & Sons Ltd.
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Fifth International Symposium on Recurrence Plot
Riley, Michael; Giuliani, Alessandro; Webber, Charles; Jr, Jr; Translational Recurrences : From Mathematical Theory to Real-World Applications
2014-01-01
This book features 13 papers presented at the Fifth International Symposium on Recurrence Plots, held August 2013 in Chicago, IL. It examines recent applications and developments in recurrence plots and recurrence quantifi cation analysis (RQA) with special emphasis on biological and cognitive systems and the analysis of coupled systems using cross-recurrence methods. Readers will discover new applications and insights into a range of systems provided by recurrence plot analysis and new theoretical and mathematical developments in recurrence plots. Recurrence plot based analysis is a powerful tool that operates on real-world complex systems that are nonlinear, non-stationary, noisy, of any statistical distribution, free of any particular model type, and not particularly long. Quantitative analyses promote the detection of system state changes, synchronized dynamical regimes, or classifi cation of system states. Th e book will be of interest to an interdisciplinary audience of recurrence plot users and researc...
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A first look at measurement error on FIA plots using blind plots in the Pacific Northwest
Susanna Melson; David Azuma; Jeremy S. Fried
2002-01-01
Measurement error in the Forest Inventory and Analysis work of the Pacific Northwest Station was estimated with a recently implemented blind plot measurement protocol. A small subset of plots was revisited by a crew having limited knowledge of the first crew's measurements. This preliminary analysis of the first 18 months' blind plot data indicates that...
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deltaPlotR: An R Package for Di?erential Item Functioning Analysis with Ango? s Delta Plot
David Magis; Bruno Facon
2014-01-01
Angoff's delta plot is a straightforward and not computationally intensive method to identify differential item functioning (DIF) among dichotomously scored items. This approach was recently improved by proposing an optimal threshold selection and by considering several item purification processes. Moreover, to support practical DIF analyses with the delta plot and these improvements, the R package deltaPlotR was also developed. The purpose of this paper is twofold: to outline the delta plot ...
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Isolation and characterization of a homogeneous isoenzyme of wheat germ acid phosphatase.
Waymack, P P; Van Etten, R L
1991-08-01
An acid phosphatase (orthophosphoric monoester phosphohydrolase, acid optimum; EC 3.1.3.2) isoenzyme from wheat germ was purified 7000-fold to homogeneity. The effect of wheat germ sources and their relationship to the isoenzyme content and purification behavior of acid phosphatases was investigated. Extensive information about the purification and stabilization of the enzyme is provided. The instability of isoenzymes in the latter stages of purification appeared to be the result of surface inactivation together with a sensitivity to dilution that could be partially offset by addition of Triton X-100 during chromatographic procedures. Added sulfhydryl protecting reagents had no effect on activity or stability, which was greatest in the pH range 4-7. The purified isoenzyme was homogeneous by polyacrylamide gel electrophoresis and exhibited the highest specific activity and turnover number reported for any acid phosphatase. The molecular weights of the pure isoenzyme and of related isoenzymes from wheat germ were found to be identical (58,000). The pure isoenzyme contained a single polypeptide chain and had a negligible carbohydrate content. The amino acid composition was determined. Of the various reasons that were considered to explain isoenzyme occurrence, a genetic basis was considered most likely. The enzyme was found to exhibit substrate inhibition with some substrates below pH 6, while above pH 8 it exhibited downwardly curving Lineweaver-Burk plots of the type that are generally described as "substrate activation". The observation of a phosphotransferase activity was consistent with the formation of a covalent phosphoenzyme intermediate, while inactivation by diethyl pyrocarbonate was consistent with the presence of an active site histidine.
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Gholivand, Khodayar; Ebrahimi Valmoozi, Ali Asghar; Bonsaii, Mahyar
2014-06-01
Novel (thio)phosphoramidate derivatives based on piperidincarboxamide with the general formula of (NH2-C(O)-C5H9N)-P(X=O,S)R1R2 (1-5) and (NH2-C(O)-C5H9N)2-P(O)R (6-9) were synthesized and characterized by (31)P, (13)C, (1)H NMR, IR spectroscopy. Furthermore, the crystal structure of compound (NH2-C(O)-C5H9N)2-P(O)(OC6H5) (6) was investigated. The activities of derivatives on cholinesterases (ChE) were determined using a modified Ellman's method. Also the mixed-type mechanisms of these compounds were evaluated by Lineweaver-Burk plots. Molecular docking and quantitative structure-activity relationship (QSAR) were used to understand the relationship between molecular structural features and anti-ChE activity, and to predict the binding affinity of phosphoramido-piperidinecarboxamides (PAPCAs) to ChE receptors. From molecular docking analysis, noncovalent interactions especially hydrogen bonding as well as hydrophobic was found between PAPCAs and ChE. Based on the docking results, appropriate molecular structural parameters were adopted to develop a QSAR model. DFT-QSAR models for ChE enzymes demonstrated the importance of electrophilicity parameter in describing the anti-AChE and anti-BChE activities of the synthesized compounds. The correlation matrix of QSAR models and docking analysis confirmed that electrophilicity descriptor can control the influence of the hydrophobic properties of P=(O, S) and CO functional groups of PAPCA derivatives in the inhibition of human ChE enzymes. Copyright © 2014 Elsevier Inc. All rights reserved.
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Aspergillus ficuum phytase activity is inhibited by cereal grain components.
Bekalu, Zelalem Eshetu; Madsen, Claus Krogh; Dionisio, Giuseppe; Brinch-Pedersen, Henrik
2017-01-01
In the current study, we report for the first time that grain components of barley, rice, wheat and maize can inhibit the activity of Aspergillus ficuum phytase. The phytase inhibition is dose dependent and varies significantly between cereal species, between cultivars of barley and cultivars of wheat and between Fusarium graminearum infected and non-infected wheat grains. The highest endpoint level of phytase activity inhibition was 90%, observed with grain protein extracts (GPE) from F. graminearum infected wheat. Wheat GPE from grains infected with F. graminearum inhibits phytase activity significantly more than GPE from non-infected grains. For four barley cultivars studied, the IC50 value ranged from 0.978 ± 0.271 to 3.616 ± 0.087 mg×ml-1. For two non-infected wheat cultivars investigated, the IC50 values were varying from 2.478 ± 0.114 to 3.038 ± 0.097 mg×ml-1. The maize and rice cultivars tested gaveIC50 values on 0.983 ± 0.205 and 1.972 ± 0.019 mg×ml-1, respectively. After purifying the inhibitor from barley grains via Superdex G200, an approximately 30-35 kDa protein was identified. No clear trend for the mechanism of inhibition could be identified via Michaelis-Menten kinetics and Lineweaver-Burk plots. However, testing of the purified phytase inhibitor together with the A. ficuum phytase and the specific protease inhibitors pepstatin A, E64, EDTA and PMSF revealed that pepstatin A repealed the phytase inhibition. This indicates that the observed inhibition of A. ficuum phytase by cereal grain extracts is caused by protease activity of the aspartic proteinase type.
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A Bayesian CUSUM plot: Diagnosing quality of treatment.
Rosthøj, Steen; Jacobsen, Rikke-Line
2017-12-01
To present a CUSUM plot based on Bayesian diagnostic reasoning displaying evidence in favour of "healthy" rather than "sick" quality of treatment (QOT), and to demonstrate a technique using Kaplan-Meier survival curves permitting application to case series with ongoing follow-up. For a case series with known final outcomes: Consider each case a diagnostic test of good versus poor QOT (expected vs. increased failure rates), determine the likelihood ratio (LR) of the observed outcome, convert LR to weight taking log to base 2, and add up weights sequentially in a plot showing how many times odds in favour of good QOT have been doubled. For a series with observed survival times and an expected survival curve: Divide the curve into time intervals, determine "healthy" and specify "sick" risks of failure in each interval, construct a "sick" survival curve, determine the LR of survival or failure at the given observation times, convert to weights, and add up. The Bayesian plot was applied retrospectively to 39 children with acute lymphoblastic leukaemia with completed follow-up, using Nordic collaborative results as reference, showing equal odds between good and poor QOT. In the ongoing treatment trial, with 22 of 37 children still at risk for event, QOT has been monitored with average survival curves as reference, odds so far favoring good QOT 2:1. QOT in small patient series can be assessed with a Bayesian CUSUM plot, retrospectively when all treatment outcomes are known, but also in ongoing series with unfinished follow-up. © 2017 John Wiley & Sons, Ltd.
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PLOT-3D, Graphics Subroutines for 3-D Surface Plots with Arbitrary Rotations
International Nuclear Information System (INIS)
Basinger, D.; Gvildys, J.
1975-01-01
1 - Description of problem or function: PLOT-3D is a package of sub- programs designed to draw three-dimensional surfaces from arrays of points (x,y,z). The surfaces can be drawn after arbitrary rotations about the three coordinate axes. 2 - Method of solution: PLOT-3D is a computer program to plot any surface for which each coordinate pair (x,y) is associated with a unique z in the set of points (x,y,z). It uses matrix transformation of the points to generate different views of the surface after arbitrary rotations about the three coordinate axes. Four versions of PLOT-3D are available. Output of version 1 and 3 is by film recorder. Output of version 2 and 4 is by CalComp plotter. Versions 3 and 4 do not draw lines which would be invisible to a viewer looking at an opaque surface, whereas versions 1 and 2 draw every line on the surface. 3 - Restrictions on the complexity of the problem: Versions 3 and 4 limit number of rows in arrays (x,y,z) to 100 and also number of columns in arrays (x,y,z) to 100
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DEFF Research Database (Denmark)
Lund, Inger-Lise; Hermansen, Anne-Mette; Ferdinand, Trine
Danskfaglig vejledning informerer om de tanker, der ligger til grund for Plot 5, og beskriver danskfaget ud fra nyere forskning inden for fagets mange delområder. Kapitelvejledning introducerer bogens tekster, forklarer hensigten med kapitlernes opgaver, giver forslag til undervisningen og brugen...
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DEFF Research Database (Denmark)
Lund, Inger-Lise; Hermansen, Anne-Mette; Ferdinand, Trine
Danskfaglig vejledning informerer om de tanker, der ligger til grund for Plot 4, og beskriver danskfaget ud fra nyere forskning inden for fagets mange delområder. Kapitelvejledning introducerer bogens tekster, forklarer hensigten med kapitlernes opgaver, giver forslag til undervisningen og brugen...
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Representing uncertainty on model analysis plots
Directory of Open Access Journals (Sweden)
Trevor I. Smith
2016-09-01
Full Text Available Model analysis provides a mechanism for representing student learning as measured by standard multiple-choice surveys. The model plot contains information regarding both how likely students in a particular class are to choose the correct answer and how likely they are to choose an answer consistent with a well-documented conceptual model. Unfortunately, Bao’s original presentation of the model plot did not include a way to represent uncertainty in these measurements. I present details of a method to add error bars to model plots by expanding the work of Sommer and Lindell. I also provide a template for generating model plots with error bars.
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Presenting simulation results in a nested loop plot.
Rücker, Gerta; Schwarzer, Guido
2014-12-12
Statisticians investigate new methods in simulations to evaluate their properties for future real data applications. Results are often presented in a number of figures, e.g., Trellis plots. We had conducted a simulation study on six statistical methods for estimating the treatment effect in binary outcome meta-analyses, where selection bias (e.g., publication bias) was suspected because of apparent funnel plot asymmetry. We varied five simulation parameters: true treatment effect, extent of selection, event proportion in control group, heterogeneity parameter, and number of studies in meta-analysis. In combination, this yielded a total number of 768 scenarios. To present all results using Trellis plots, 12 figures were needed. Choosing bias as criterion of interest, we present a 'nested loop plot', a diagram type that aims to have all simulation results in one plot. The idea was to bring all scenarios into a lexicographical order and arrange them consecutively on the horizontal axis of a plot, whereas the treatment effect estimate is presented on the vertical axis. The plot illustrates how parameters simultaneously influenced the estimate. It can be combined with a Trellis plot in a so-called hybrid plot. Nested loop plots may also be applied to other criteria such as the variance of estimation. The nested loop plot, similar to a time series graph, summarizes all information about the results of a simulation study with respect to a chosen criterion in one picture and provides a suitable alternative or an addition to Trellis plots.
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Contour plotting programs for printer and Calcomp plotter
International Nuclear Information System (INIS)
Moller, P.
1980-07-01
Contour plotting programs for plotting contour diagrams on printers or Calcomp plotters are described. The subroutines also exist in versions that are useful for the special application of finding minima and saddlepoints of nuclear potential energy surfaces generated by the subroutine PETR3 of another program package. For the general user, however, the most interesting aspect of the plotting package is probably the possibility of generating printer contour plots. The plotting of printer contour plots is a very fast and convenient way of displaying two-dimensional functions. 3 figures
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DEFF Research Database (Denmark)
Sandvik, Kjetil
Den velfungerende krimi faciliterer denne dobbelte plotlæsning ved at muliggøre en særlig form for legende og udforskende interaktion mellem læser og plot: Ved at lægge spor ud og holde tolkningsmuligheder og løsningsmuligheder åbne får vi mulighed for at påtage os og udføre opklaringsarbejdet side......, der inviterer os med ind i selve handlingens rum og forløb og giver os forskellige handlingsmuligheder i forhold til disse. I bogen omtales denne særlige form for plot for forlystelsesplot med henvisning til forlystelsesparken og den særlige form for interaktiv fortælleform, som vi finder der: en...
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Experimental strategies in carrying out VCU for tobacco crop I: plot design and size.
Toledo, F H R B; Ramalho, M A P; Pulcinelli, C E; Bruzi, A T
2013-09-19
We aimed to establish standards for tobacco Valor de Cultivo e Uso (VCU) in Brazil. We obtained information regarding the size and design of plots of two varietal groups of tobacco (Virginia and Burley). Ten inbred lines of each varietal group were evaluated in a randomized complete block design with four replications. The plot contained 42 plants with six rows of seven columns each. For each experiment plant, considering the position of the respective plant in the plot (row and column) as a reference, cured leaf weight (g/plant), total sugar content (%), and total alkaloid content (%) were determined. The maximum curvature of the variations in coefficients was estimated. Trials with the number of plants per plot ranging from 2 to 41 were simulated. The use of a border was not justified because the interactions between inbred lines x position in the plots were never significant, showing that the behavior of the inbred lines coincided with the different positions. The plant performance varied according to the column position in the plot. To lessen the effect of this factor, the use of plots with more than one row is recommended. Experimental precision, evaluated by the CV%, increased with an increase in plot size; nevertheless, the maximum curvature of the variation coefficient method showed no expressive increase in precision if the number of plants was greater than seven. The result in identification of the best inbred line, in terms of the size of each plot, coincided with the maximum curvature method.
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Multiscale Poincaré plots for visualizing the structure of heartbeat time series.
Henriques, Teresa S; Mariani, Sara; Burykin, Anton; Rodrigues, Filipa; Silva, Tiago F; Goldberger, Ary L
2016-02-09
Poincaré delay maps are widely used in the analysis of cardiac interbeat interval (RR) dynamics. To facilitate visualization of the structure of these time series, we introduce multiscale Poincaré (MSP) plots. Starting with the original RR time series, the method employs a coarse-graining procedure to create a family of time series, each of which represents the system's dynamics in a different time scale. Next, the Poincaré plots are constructed for the original and the coarse-grained time series. Finally, as an optional adjunct, color can be added to each point to represent its normalized frequency. We illustrate the MSP method on simulated Gaussian white and 1/f noise time series. The MSP plots of 1/f noise time series reveal relative conservation of the phase space area over multiple time scales, while those of white noise show a marked reduction in area. We also show how MSP plots can be used to illustrate the loss of complexity when heartbeat time series from healthy subjects are compared with those from patients with chronic (congestive) heart failure syndrome or with atrial fibrillation. This generalized multiscale approach to Poincaré plots may be useful in visualizing other types of time series.
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Master plot analysis of microcracking in graphite/epoxy and graphite/PEEK laminates
Nairn, John A.; Hu, Shoufeng; Bark, Jong Song
1993-01-01
We used a variational stress analysis and an energy release rate failure criterion to construct a master plot analysis of matrix microcracking. In the master plot, the results for all laminates of a single material are predicted to fall on a single line whose slope gives the microcracking toughness of the material. Experimental results from 18 different layups of AS4/3501-6 laminates show that the master plot analysis can explain all observations. In particular, it can explain the differences between microcracking of central 90 deg plies and of free-surface 90 deg plies. Experimental results from two different AS4/PEEK laminates tested at different temperatures can be explained by a modified master plot that accounts for changes in the residual thermal stresses. Finally, we constructed similar master plot analyses for previous literature microcracking models. All microcracking theories that ignore the thickness dependence of the stresses gave poor results.
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Multiple hypothesis clustering in radar plot extraction
Huizing, A.G.; Theil, A.; Dorp, Ph. van; Ligthart, L.P.
1995-01-01
False plots and plots with inaccurate range and Doppler estimates may severely degrade the performance of tracking algorithms in radar systems. This paper describes how a multiple hypothesis clustering technique can be applied to mitigate the problems involved in plot extraction. The measures of
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Storytelling in Earth sciences: The eight basic plots
Phillips, Jonathan
2012-11-01
Reporting results and promoting ideas in science in general, and Earth science in particular, is treated here as storytelling. Just as in literature and drama, storytelling in Earth science is characterized by a small number of basic plots. Though the list is not exhaustive, and acknowledging that multiple or hybrid plots and subplots are possible in a single piece, eight standard plots are identified, and examples provided: cause-and-effect, genesis, emergence, destruction, metamorphosis, convergence, divergence, and oscillation. The plots of Earth science stories are not those of literary traditions, nor those of persuasion or moral philosophy, and deserve separate consideration. Earth science plots do not conform those of storytelling more generally, implying that Earth scientists may have fundamentally different motivations than other storytellers, and that the basic plots of Earth Science derive from the characteristics and behaviors of Earth systems. In some cases preference or affinity to different plots results in fundamentally different interpretations and conclusions of the same evidence. In other situations exploration of additional plots could help resolve scientific controversies. Thus explicit acknowledgement of plots can yield direct scientific benefits. Consideration of plots and storytelling devices may also assist in the interpretation of published work, and can help scientists improve their own storytelling.
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International Nuclear Information System (INIS)
Hnatowich, M.; LaBella, F.S.
1982-01-01
Erythrosine (ERY) (FD and C red no. 3) inhibited specific binding of [ 3 H]ouabain to rat brain homogenates with an IC50 of 23 mM in the dark and 1 mM in ordinary fluorescent light. Competition studies demonstrated the presence of two components, only one of which was affected by light. Lineweaver-Burk analysis indicated that ERY preferentially antagonizes [ 3 H]ouabain binding at a high-affinity site in the light, whereas in the dark the dye inhibits binding in a manner qualitatively similar to inhibition by ouabain. Light enhancement of ERY potency occurred only when dye and tissue were present together in the incubation medium, pointing to participation of transient molecular species. However, neither superoxide dismutase nor catalase altered the effects of ERY in the light or dark, suggesting the absence of oxygen free radicals. In contrast to brain, membranes from guinea pig heart showed only one binding site for [ 3 H]ouabain, and antagonism by ERY at this site was markedly enhanced by light. Structural differences between classes of ouabain binding regions probably accounts for the discrimination exhibited by ERY in the presence of light and oxygen. Our findings also caution that metabolic transformation of this common food dye, light decomposition, or photoreaction with foodstuff may yield more toxic derivatives
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Directory of Open Access Journals (Sweden)
Zahra Ghobadi Nejad
2014-01-01
Full Text Available Due to great commercial application of protease, it is necessary to study kinetic characterization of this enzyme in order to improve design of enzymatic reactors. In this study, mathematical modeling of protease enzyme production kinetics which is derived from Bacillus licheniformis BBRC 100053 was studied (at 37°C, pH 10 after 73 h in stationary phase, and 150 rpm. The aim of the present paper was to determine the best kinetic model and kinetic parameters for production of protease and calculating Ki (inhibition constant of different inhibitors to find the most effective one. The kinetic parameters Km (Michaelis-Menten constant and Vm (maximum rate were calculated 0.626 mM and 0.0523 mM/min. According to the experimental results, using DFP (diisopropyl fluorophosphate and PMSF (phenylmethanesulfonyl fluoride as inhibitors almost 50% of the enzyme activity could be inhibited when their concentrations were 0.525 and 0.541 mM, respectively. Ki for DFP and PMSF were 0.46 and 0.56 mM, respectively. Kinetic analysis showed that the Lineweaver-Burk model was the best fitting model for protease production kinetics DFP was more effective than PMSF and both of them should be covered in the group of noncompetitive inhibitors.
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Vegetation resurvey is robust to plot location uncertainty
Kopecký, Martin; Macek, Martin
2017-01-01
Aim Resurveys of historical vegetation plots are increasingly used for the assessment of decadal changes in plant species diversity and composition. However, historical plots are usually relocated only approximately. This potentially inflates temporal changes and undermines results. Location Temperate deciduous forests in Central Europe. Methods To explore if robust conclusions can be drawn from resurvey studies despite location uncertainty, we compared temporal changes in species richness, frequency, composition and compositional heterogeneity between exactly and approximately relocated plots. We hypothesized that compositional changes should be lower and changes in species richness should be less variable on exactly relocated plots, because pseudo-turnover inflates temporal changes on approximately relocated plots. Results Temporal changes in species richness were not more variable and temporal changes in species composition and compositional heterogeneity were not higher on approximately relocated plots. Moreover, the frequency of individual species changed similarly on both plot types. Main conclusions The resurvey of historical vegetation plots is robust to uncertainty in original plot location and, when done properly, provides reliable evidence of decadal changes in plant communities. This provides important background for other resurvey studies and opens up the possibility for large-scale assessments of plant community change. PMID:28503083
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Mo, Zhi-Zhun; Wang, Xiu-Fen; Zhang, Xie; Su, Ji-Yan; Chen, Hai-Ming; Liu, Yu-Hong; Zhang, Zhen-Biao; Xie, Jian-Hui; Su, Zi-Ren
2015-07-16
The inhibitory effect of andrographolide sodium bisulphite (ASB) on jack bean urease (JBU) and Helicobacter pylori urease (HPU) was performed to elucidate the inhibitory potency, kinetics and mechanism of inhibition in 20 mM phosphate buffer, pH 7.0, 2 mM EDTA, 25 °C. The ammonia formations, indicator of urease activity, were examined using modified spectrophotometric Berthelot (phenol-hypochlorite) method. The inhibitory effect of ASB was characterized with IC50 values. Lineweaver-Burk and Dixon plots for JBU inhibition of ASB was constructed from the kinetic data. SH-blocking reagents and competitive active site Ni2+ binding inhibitors were employed for mechanism study. Molecular docking technique was used to provide some information on binding conformations as well as confirm the inhibition mode. The IC50 of ASB against JBU and HPU was 3.28±0.13 mM and 3.17±0.34 mM, respectively. The inhibition proved to be competitive and concentration- dependent in a slow-binding progress. The rapid formation of initial ASB-JBU complex with an inhibition constant of Ki=2.86×10(-3) mM was followed by a slow isomerization into the final complex with an overall inhibition constant of Ki*=1.33×10(-4) mM. The protective experiment proved that the urease active site is involved in the binding of ASB. Thiol reagents (L-cysteine and dithiothreithol) strongly protect the enzyme from the loss of enzymatic activity, while boric acid and fluoride show weaker protection, indicating that the active-site sulfhydryl group of JBU was potentially involved in the blocking process. Moreover, inhibition of ASB proved to be reversible since ASB-inactivated JBU could be reactivated by dithiothreitol application. Molecular docking assay suggested that ASB made contacts with the important sulfhydryl group Cys-592 residue and restricted the mobility of the active-site flap. ASB was a competitive inhibitor targeting thiol groups of urease in a slow-binding manner both reversibly and concentration
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Mosbah, Habib; Chahdoura, Hassiba; Kammoun, Jannet; Hlila, Malek Besbes; Louati, Hanen; Hammami, Saoussen; Flamini, Guido; Achour, Lotfi; Selmi, Boulbaba
2018-03-05
α-glucosidase is a therapeutic target for diabetes mellitus (DM) and α-glucosidase inhibitors play a vital role in the treatments for the disease. Furthermore, xanthine oxidase (XO) is a key enzyme that catalyzes hypoxanthine and xanthine to uric acid which at high levels can lead to hyperuricemia which is an important cause of gout. Pancreatic lipase (PL) secreted into the duodenum plays a key role in the digestion and absorption of fats. For its importance in lipid digestion, PL represents an attractive target for obesity prevention. The flowers essential oil of Rhaponticum acaule (L) DC (R. acaule) was characterized using gas chromatography-mass spectrometry (GC-MS). The antioxidant activities of R. acaule essential oil (RaEO) were also determined using 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), reducing power, phosphomolybdenum, and DNA nicking assays. The inhibitory power of RaEO against α-glucosidase, xanthine oxidase and pancreatic lipase was evaluated. Enzyme kinetic studies using Michaelis-Menten and the derived Lineweaver-Burk (LB) plots were performed to understand the possible mechanism of inhibition exercised by the components of this essential oil. The result revealed the presence of 26 compounds (97.4%). The main constituents include germacrene D (49.2%), methyl eugenol (8.3%), (E)-β-ionone (6.2%), β-caryophyllene (5.7%), (E,E)-α-farnesene (4.2%), bicyclogermacrene (4.1%) and (Z)-α-bisabolene (3.7%). The kinetic inhibition study showed that the essential oil demonstrated a strong α-glucosidase inhibiton and it was a mixed inhibitor. On the other hand, our results evidenced that this oil exhibited important xanthine oxidase inhibitory effect, behaving as a non-competitive inhibitor. The essential oil inhibited the turkey pancreatic lipase, with maximum inhibition of 80% achieved at 2 mg/mL. Furthermore, the inhibition of turkey pancreatic lipase by RaEO was an irreversible one. The results revealed that the RaEO is a new
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Including the Tukey Mean-Difference (Bland-Altman) Plot in a Statistics Course
Kozak, Marcin; Wnuk, Agnieszka
2014-01-01
The Tukey mean-difference plot, also called the Bland-Altman plot, is a recognized graphical tool in the exploration of biometrical data. We show that this technique deserves a place on an introductory statistics course by encouraging students to think about the kind of graph they wish to create, rather than just creating the default graph for the…
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FLOWPLOT2, 2-D, 3-D Fluid Dynamic Plots
International Nuclear Information System (INIS)
Cobb, C.K.; Tunstall, J.N.
1989-01-01
1 - Description of program or function: FLOWPLOT2 is a plotting program used with numerical or analytical fluid dynamics codes to create velocity vector plots, contour plots of up to three fluid parameters (e.g. pressure, density, and temperature), two-dimensional profile plots, three-dimensional curve plots, and/or three-dimensional surface plots for either the u or v velocity components. If the fluid dynamics code computes a transient or simulated time related solution, FLOWPLOT2 can also be used to generate these plots for any specified time interval. Multiple cases generating different plots for different time intervals may be run in one execution of the program. In addition, plots can be created for selected two- dimensional planes of three-dimensional steady-state problems. The user has the option of producing plots on CalComp or Versatec plotters or microfiche and of creating a compressed dataset before plotting. 2 - Method of solution: FLOWPLOT2 reads a dataset written by the fluid dynamics code. This dataset must be written in a specified format and must contain parametric data at the nodal points of a uniform or non-uniform rectangular grid formed by the intersection of the grid lines of the model. 3 - Restrictions on the complexity of the problem - Maxima of: 2500 nodes, 40 y-values for 2-D profile plots and 3-D curve plots, 20 contour values, 3 fluid parameters
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Evaluating Plot Designs for the Tropics
Paul C. van Deusen; Bruce Bayle
1991-01-01
Theory and procedures are reviewed for determining the best type of plot for a given forest inventory. A general methodology is given that clarifies the relationship between different plot designs and the associated methods to produce the inventory estimates.
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Emotional characters for automatic plot creation
Theune, Mariet; Rensen, S.; op den Akker, Hendrikus J.A.; Heylen, Dirk K.J.; Nijholt, Antinus; Göbel, S.; Spierling, U.; Hoffmann, A.; Iurgel, I.; Schneider, O.; Dechau, J.; Feix, A.
The Virtual Storyteller is a multi-agent framework for automatic story generation. In this paper we describe how plots emerge from the actions of semi-autonomous character agents, focusing on the influence of the characters’ emotions on plot development.
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Behavior of QQ-plots and genomic control in studies of gene-environment interaction.
Directory of Open Access Journals (Sweden)
Arend Voorman
2011-05-01
Full Text Available Genome-wide association studies of gene-environment interaction (GxE GWAS are becoming popular. As with main effects GWAS, quantile-quantile plots (QQ-plots and Genomic Control are being used to assess and correct for population substructure. However, in G x E work these approaches can be seriously misleading, as we illustrate; QQ-plots may give strong indications of substructure when absolutely none is present. Using simulation and theory, we show how and why spurious QQ-plot inflation occurs in G x E GWAS, and how this differs from main-effects analyses. We also explain how simple adjustments to standard regression-based methods used in G x E GWAS can alleviate this problem.
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Application of mapped plots for single-owner forest surveys
Paul C. Van Deusen; Francis Roesch
2009-01-01
Mapped plots are used for the nation forest inventory conducted by the U.S. Forest Service. Mapped plots are also useful foro single ownership inventoires. Mapped plots can handle boundary overlap and can aprovide less variable estimates for specified forest conditions. Mapping is a good fit for fixed plot inventories where the fixed area plot is used for both mapping...
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Using recurrence plot analysis for software execution interpretation and fault detection
Mosdorf, M.
2015-09-01
This paper shows a method targeted at software execution interpretation and fault detection using recurrence plot analysis. In in the proposed approach recurrence plot analysis is applied to software execution trace that contains executed assembly instructions. Results of this analysis are subject to further processing with PCA (Principal Component Analysis) method that simplifies number coefficients used for software execution classification. This method was used for the analysis of five algorithms: Bubble Sort, Quick Sort, Median Filter, FIR, SHA-1. Results show that some of the collected traces could be easily assigned to particular algorithms (logs from Bubble Sort and FIR algorithms) while others are more difficult to distinguish.
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Nebbia, Carlo; Girolami, Flavia; Carletti, Monica; Gasco, Laura; Zoccarato, Ivo; Giuliano Albo, Alessandra
2017-10-05
Malachite green (MG) has been widely used in aquaculture to treat a number of microbial and parasitic diseases. It is currently banned in the EU because of the high cytotoxicity and carcinogenic activity, which is also shared by leucomalachite green (LMG), a reduced MG metabolite that can persist in fish tissues for months. There is scant information about the ability of either compound to interact with drug metabolizing enzymes in fish. Therefore we evaluated the in vitro effects of MG and LMG (25, 50 and 100μM) on some DMEs and glutathione (GSH) content in rainbow trout liver subfractions. LMG did not affect any of the examined parameters. In contrast, MG proved to deplete GSH and to depress to a various extent the activities of NAD(P)H cytochrome c reductase, 7-ethoxycoumarin O-deethylase, 1-naphthol uridindiphosphoglucuronyl-transferase and maximally those of 7-ethoxyresorufin O-deethylase (EROD) and glutathione S-transferase (GST) accepting 1-chloro2,4-dinitrobenzene (CDNB) as substrate. The inhibition mechanisms of EROD and GST were investigated by means of non-linear Michaelis-Menten kinetics and Lineweaver-Burk plots using 0.175-8μM MG. The calculated IC 50 for EROD was 7.1μM, and the inhibition appeared to be competitive (K i 2.78±0.24μM). In the case of GST, the calculated IC 50 was 0.53μM. The inhibition was best described as competitive toward GSH (Ki 0.39±0.02μM) and of mixed-type toward CDNB (Ki 0.64±0.06μM). Our findings indicate that, contrary to LMG, MG behaves as a relatively strong inhibitor of certain liver DMEs and can reversibly bind GSH. Copyright © 2017 Elsevier B.V. All rights reserved.
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Directory of Open Access Journals (Sweden)
Ylva Pernow
2018-06-01
Full Text Available It has been demonstrated, that long-term chronic tryptophan deficiency, results in decreased serotonin synthesis, which may lead to low bone mass and low bone formation. Findings from studies in male patients with idiopathic osteoporosis suggested a decreased transport of tryptophan in erythrocytes of osteoporotic patients, indicating that serotonin system defects may be involved in the etiology of low bone mass. Tryptophan is the precursor of serotonin, and a disturbed transport of tryptophan is implicated in altered serotonin synthesis. However, no study has investigated the tryptophan transport kinetics in MIO patients. The aim of this study is to investigate the kinetic parameters of tryptophan transport in fibroblasts derived from MIO patients compared to age and sex matched controls.Fibroblast cells were cultured from skin biopsies obtained from 14 patients diagnosed with Male Idiopathic Osteoporosis and from 13 healthy age-sex matched controls, without a diagnosis of osteoporosis. Transport of the amino acid tryptophan across the cell membrane was measured by the cluster tray method. The kinetic parameters, maximal transport capacity (Vmax and affinity constant (Km were determined by using the Lineweaver-Burke plot equation.The results of this study have shown a significantly lower mean value for Vmax (p=0.0138 and lower Km mean value (p=0.0009 of tryptophan transport in fibroblasts of MIO patients compared to the control group. A lower Vmax implied a decreased tryptophan transport availability in MIO patients.In conclusion, reduced cellular tryptophan availability in MIO patients might result in reduced brain serotonin synthesis and its endogenous levels in peripheral tissues, and this may contribute to low bone mass/formation. The findings of the present study could contribute to the etiology of idiopathic osteoporosis and for the development of novel approaches for diagnosis, treatment and management strategies of MIO. Keywords: Male
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Phospholipase B activity of a purified phospholipase A from Vipera palestinae venom.
Shiloah, J; Klibansky, C; de Vries, A; Berger, A
1973-05-01
Phospholipase was isolated (in two fractions) from Vipera palestinae venom and it was shown to possess phospholipase A activity (hydrolyzing diacyl-sn-glycerophosphorylcholines, e.g., lecithin, in the 2-position) as well as lysophospholipase (phospholipase B) activity (hydrolyzing 1-monoacyl-sn-glycerophosphorylcholines, e.g., lysolecithin, yielding free fatty acid and glycerophosphorylcholine). Each of the two purified enzyme fractions was homogeneous as judged by electrophoresis on acrylamide gel and by immunodiffusion and immunoelectrophoresis, and both had essentially equal activities. The ratio of the specific activity, at various purification stages, to the specific activity of the whole venom was the same for A activity (substrate lecithin) as for B activity (substrate lysolecithin). The enzyme has a molecular weight of 16,000, six S-S bridges, and no free thiol groups. At pH 7, dimerization was observed in the ultracentrifuge. A dissociation constant of about 10(-5) m was estimated. The amino acid composition for both fractions (140 amino acid residues) was found to be essentially the same. The A activity had a pH optimum at 9; B activity was low at this pH but increased steadily beyond pH 10.5. For the hydrolysis of lysolecithin the Lineweaver-Burk plot was found to be linear, giving K(m) = 1.1 mm and k(cat) = 0.55 sec(-1) at 37 degrees C and pH 10. 2-Deoxylysolecithin was also hydrolyzed by the enzyme at pH 10, with k(cat) = 0.01 sec(-1) (zero-order kinetics in the range 0.5-2.5 mm). For lecithin these constants could not be determined, but at 0.25 mm substrate the hydrolysis rate (at pH 9) of lecithin was about 1000 times the hydrolysis rate of lysolecithin (at pH 10).
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International Nuclear Information System (INIS)
Pan, Huiling; Hu, Qian; Wang, Jingyuan; Liu, Zehui; Wu, Dang; Lu, Weiqiang; Huang, Jin
2016-01-01
Human inosine 5′-monophosphate dehydrogenase (hIMPDH) is a rate-limiting enzyme in the de novo biosynthetic pathway of purine nucleotides, playing crucial roles in cellular proliferation, differentiation, and transformation. Dysregulation of hIMPDH expression and activity have been found in a variety of human cancers including leukemia. In this study, we found that myricetin, a naturally occurring phytochemical existed in berries, wine and tea, was a novel inhibitor of human type 1 and type 2 IMPDH (hIMPDH1/2) with IC_5_0 values of 6.98 ± 0.22 μM and 4.10 ± 0.14 μM, respectively. Enzyme kinetic analysis using Lineweaver-Burk plot revealed that myricetin is a mix-type inhibitor for hIMPDH1/2. Differential scanning fluorimetry and molecular docking simulation data demonstrate that myricetin is capable of binding with hIMPDH1/2. Myricetin treatment exerts potent anti-proliferative and pro-apoptotic effects on K562 human leukemia cells in a dose-dependent manner. Importantly, cytotoxicity of myricetin on K562 cells were markedly attenuated by exogenous addition of guanosine, a salvage pathway of maintaining intracellular pool of guanine nucleotides. Taking together, these results indicate that natural product myricetin exhibits potent anti-leukemia activity by interfering with purine nucleotides biosynthetic pathway through the suppression of hIMPDH1/2 catalytic activity. - Highlights: • Myricetin, a common dietary flavonoid, is a novel inhibitor of hIMPDH1/2. • Myricetin directly binds with hIMPDH1/2 and induces cell cycle arrest and apoptosis of leukemia cells. • The cytotoxicity of myricetin on K562 cells is markedly attenuated by exogenous addition of guanosine.
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Energy Technology Data Exchange (ETDEWEB)
Pan, Huiling; Hu, Qian; Wang, Jingyuan; Liu, Zehui; Wu, Dang [Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237 (China); Lu, Weiqiang, E-mail: wqlu@bio.ecnu.edu.cn [Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241 (China); Huang, Jin, E-mail: huangjin@ecust.edu.cn [Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237 (China)
2016-09-02
Human inosine 5′-monophosphate dehydrogenase (hIMPDH) is a rate-limiting enzyme in the de novo biosynthetic pathway of purine nucleotides, playing crucial roles in cellular proliferation, differentiation, and transformation. Dysregulation of hIMPDH expression and activity have been found in a variety of human cancers including leukemia. In this study, we found that myricetin, a naturally occurring phytochemical existed in berries, wine and tea, was a novel inhibitor of human type 1 and type 2 IMPDH (hIMPDH1/2) with IC{sub 50} values of 6.98 ± 0.22 μM and 4.10 ± 0.14 μM, respectively. Enzyme kinetic analysis using Lineweaver-Burk plot revealed that myricetin is a mix-type inhibitor for hIMPDH1/2. Differential scanning fluorimetry and molecular docking simulation data demonstrate that myricetin is capable of binding with hIMPDH1/2. Myricetin treatment exerts potent anti-proliferative and pro-apoptotic effects on K562 human leukemia cells in a dose-dependent manner. Importantly, cytotoxicity of myricetin on K562 cells were markedly attenuated by exogenous addition of guanosine, a salvage pathway of maintaining intracellular pool of guanine nucleotides. Taking together, these results indicate that natural product myricetin exhibits potent anti-leukemia activity by interfering with purine nucleotides biosynthetic pathway through the suppression of hIMPDH1/2 catalytic activity. - Highlights: • Myricetin, a common dietary flavonoid, is a novel inhibitor of hIMPDH1/2. • Myricetin directly binds with hIMPDH1/2 and induces cell cycle arrest and apoptosis of leukemia cells. • The cytotoxicity of myricetin on K562 cells is markedly attenuated by exogenous addition of guanosine.
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Directory of Open Access Journals (Sweden)
Jia-ying Xin
2017-01-01
Full Text Available The synthesis of L-ascorbyl flurbiprofenate was achieved by esterification and transesterification in nonaqueous organic medium with Novozym 435 lipase as biocatalyst. The conversion was greatly influenced by the kinds of organic solvents, speed of agitation, catalyst loading amount, reaction time, and molar ratio of acyl donor to L-ascorbic acid. A series of solvents were investigated, and tert-butanol was found to be the most suitable from the standpoint of the substrate solubility and the conversion for both the esterification and transesterification. When flurbiprofen was used as acyl donor, 61.0% of L-ascorbic acid was converted against 46.4% in the presence of flurbiprofen methyl ester. The optimal conversion of L-ascorbic acid was obtained when the initial molar ratio of acyl donor to ascorbic acid was 5 : 1. kinetics parameters were solved by Lineweaver-Burk equation under nonsubstrate inhibition condition. Since transesterification has lower conversion, from the standpoint of productivity and the amount of steps required, esterification is a better method compared to transesterification.
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Phenols removal by immobilized tyrosinase reactor in on-line high performance liquid chromatography
Energy Technology Data Exchange (ETDEWEB)
Girelli, Anna Maria [Dipartimento di Chimica, Universita degli Studi di Roma ' La Sapienza' , P.le Aldo Moro 5, 00185 Rome (Italy)]. E-mail: annamaria.girelli@uniroma1.it; Mattei, Enrico [Dipartimento di Chimica, Universita degli Studi di Roma ' La Sapienza' , P.le Aldo Moro 5, 00185 Rome (Italy); Messina, Antonella [Dipartimento di Chimica, Universita degli Studi di Roma ' La Sapienza' , P.le Aldo Moro 5, 00185 Rome (Italy)
2006-11-24
The development of an immobilized enzyme reactor (IMER) based on tyrosinase immobilized on aminopropyl-controlled pore glass (AP-CPG) for the removal of phenols from model aqueous solutions was reported. To elucidate the influence of the substrate nature, the apparent (V{sup '}{sub max}, K{sup '}{sub m}) and the inherent (V{sub max}, K{sub m}) Michaelis-Menten constants were determined by Lineweaver-Burk method and the external diffusional contributions on measured enzyme activities were removed by a graphical method. The dephenolization process was realized by recycling the phenol solutions through the bioreactor connected to a chitosan trap in order to remove the colored quinone-type products of the tyrosinase reactions. The results indicated that a complete removal of phenol derivatives in the range of 150-300min, with the exception of 60% removal for phenol reached in 400min, was obtained. The observed sequence: cresol>4-methylcathecol>catechol>4-Cl-phenol-bar phenol was in accordance to the V{sup '}{sub max}/K{sup '}{sub m} values.
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Box Plots in the Australian Curriculum
Watson, Jane M.
2012-01-01
This article compares the definition of "box plot" as used in the "Australian Curriculum: Mathematics" with other definitions used in the education community; describes the difficulties students experience when dealing with box plots; and discusses the elaboration that is necessary to enable teachers to develop the knowledge…
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Hullman, Jessica; Resnick, Paul; Adar, Eytan
2015-01-01
Many visual depictions of probability distributions, such as error bars, are difficult for users to accurately interpret. We present and study an alternative representation, Hypothetical Outcome Plots (HOPs), that animates a finite set of individual draws. In contrast to the statistical background required to interpret many static representations of distributions, HOPs require relatively little background knowledge to interpret. Instead, HOPs enables viewers to infer properties of the distribution using mental processes like counting and integration. We conducted an experiment comparing HOPs to error bars and violin plots. With HOPs, people made much more accurate judgments about plots of two and three quantities. Accuracy was similar with all three representations for most questions about distributions of a single quantity.
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Fungicides transport in runoff from vineyard plot and catchment: contribution of non-target areas.
Lefrancq, Marie; Payraudeau, Sylvain; García Verdú, Antonio Joaquín; Maillard, Elodie; Millet, Maurice; Imfeld, Gwenaël
2014-04-01
Surface runoff and erosion during the course of rainfall events are major processes of pesticides transport from agricultural land to aquatic ecosystem. These processes are generally evaluated either at the plot or the catchment scale. Here, we compared at both scales the transport and partitioning in runoff water of two widely used fungicides, i.e., kresoxim-methyl (KM) and cyazofamid (CY). The objective was to evaluate the relationship between fungicides runoff from the plot and from the vineyard catchment. The results show that seasonal exports for KM and CY at the catchment were larger than those obtained at the plot. This underlines that non-target areas within the catchment largely contribute to the overall load of runoff-associated fungicides. Estimations show that 85 and 62 % of the loads observed for KM and CY at the catchment outlet cannot be explained by the vineyard plots. However, the partitioning of KM and CY between three fractions, i.e., the suspended solids (>0.7 μm) and two dissolved fractions (i.e., between 0.22 and 0.7 µm and plot scales enable to evaluate the sources areas of pesticide off-site transport.
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Split-plot fractional designs: Is minimum aberration enough?
DEFF Research Database (Denmark)
Kulahci, Murat; Ramirez, Jose; Tobias, Randy
2006-01-01
Split-plot experiments are commonly used in industry for product and process improvement. Recent articles on designing split-plot experiments concentrate on minimum aberration as the design criterion. Minimum aberration has been criticized as a design criterion for completely randomized fractional...... factorial design and alternative criteria, such as the maximum number of clear two-factor interactions, are suggested (Wu and Hamada (2000)). The need for alternatives to minimum aberration is even more acute for split-plot designs. In a standard split-plot design, there are several types of two...... for completely randomized designs. Consequently, we provide a modified version of the maximum number of clear two-factor interactions design criterion to be used for split-plot designs....
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Experts’ Misinterpretation of Box Plots – a Dual Processing Approach
Directory of Open Access Journals (Sweden)
Stephanie Lem
2014-11-01
Full Text Available Recent studies have shown that students often misinterpret the area of the box in box plots as representing the frequency or proportion of observations in that interval, while it actually represents density. This misinterpretation has been shown to be based on the saliency of this area and can be explained by heuristic reasoning as defined by dual process theories. In this study we tested whether expert users of box plots also display this misinterpretation and show signs of the same heuristic reasoning as found in students. Using a reaction time test, we found signs of heuristic reasoning in experts, both with respect to accuracy and reaction times. If even experts have difficulty interpreting box plots, one can question whether these are an appropriate form of representation to use when reporting data and deserve the prominent place they currently have in the statistics curriculum.
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Directory of Open Access Journals (Sweden)
Jessica Hullman
Full Text Available Many visual depictions of probability distributions, such as error bars, are difficult for users to accurately interpret. We present and study an alternative representation, Hypothetical Outcome Plots (HOPs, that animates a finite set of individual draws. In contrast to the statistical background required to interpret many static representations of distributions, HOPs require relatively little background knowledge to interpret. Instead, HOPs enables viewers to infer properties of the distribution using mental processes like counting and integration. We conducted an experiment comparing HOPs to error bars and violin plots. With HOPs, people made much more accurate judgments about plots of two and three quantities. Accuracy was similar with all three representations for most questions about distributions of a single quantity.
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The Heuristic Interpretation of Box Plots
Lem, Stephanie; Onghena, Patrick; Verschaffel, Lieven; Van Dooren, Wim
2013-01-01
Box plots are frequently used, but are often misinterpreted by students. Especially the area of the box in box plots is often misinterpreted as representing number or proportion of observations, while it actually represents their density. In a first study, reaction time evidence was used to test whether heuristic reasoning underlies this…
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System for histogram entry, retrieval, and plotting
International Nuclear Information System (INIS)
Kellogg, M.; Gallup, J.M.; Shlaer, S.; Spencer, N.
1977-10-01
This manual describes the systems for producing histograms and dot plots that were designed for use in connection with the Q general-purpose data-acquisition system. These systems allow for the creation of histograms; the entry, retrieval, and plotting of data in the form of histograms; and the dynamic display of scatter plots as data are acquired. Although the systems are designed for use with Q, they can also be used as a part of other applications. 3 figures
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PLOTTAB, Curve and Point Plotting with Error Bars
International Nuclear Information System (INIS)
1999-01-01
1 - Description of program or function: PLOTTAB is designed to plot any combination of continuous curves and/or discrete points (with associated error bars) using user supplied titles and X and Y axis labels and units. If curves are plotted, the first curve may be used as a standard; the data and the ratio of the data to the standard will be plotted. 2 - Method of solution: PLOTTAB: The program has no idea of what data is being plotted and yet by supplying titles, X and Y axis labels and units the user can produce any number of plots with each plot containing almost any combination of curves and points with each plot properly identified. In order to define a continuous curve between tabulated points, this program must know how to interpolate between points. By input the user may specify either the default option of linear x versus linear y interpolation or alternatively log x and/or log Y interpolation. In all cases, regardless of the interpolation specified, the program will always interpolate the data to the plane of the plot (linear or log x and y plane) in order to present the true variation of the data between tabulated points, based on the user specified interpolation law. Tabulated points should be tabulated at a sufficient number of x values to insure that the difference between the specified interpolation and the 'true' variation of a curve between tabulated values is relatively small. 3 - Restrictions on the complexity of the problem: A combination of up to 30 curves and sets of discrete points may appear on each plot. If the user wishes to use this program to compare different sets of data, all of the data must be in the same units
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Moradi, M; Divsalar, A; Saboury, A A; Ghalandari, B; Harifi, A R
2015-01-01
Deferasirox (DFX), as an oral chelator, is used for treatment of transfusional iron overload. In this study, we have investigated the effects of DFX as an iron chelator, on the function and structure of bovine liver catalase (BLC) by different spectroscopic methods of UV-visible, fluorescence, and circular dichroism (CD) at two temperatures of 25 and 37 °C. In vitro kinetic studies showed that DFX can inhibit the enzymatic activity in a competitive manner. KI value was calculated 39 nM according to the Lineweaver-Burk plot indicating a high rate of inhibition of the enzyme. Intrinsic fluorescence data showed that increasing the drug concentrations leads to a significant decrease in the intrinsic emission of the enzyme indicating a significant change in the three-dimensional environment around the chromophores of the enzyme structure. By analyzing the fluorescence quenching data, it was found that the BLC has two binding sites for DFX and the values of binding constant at 25 and 37 °C were calculated 1.7 × 10(7) and 3 × 10(7) M(-1), respectively. The static type of quenching mechanism is involved in the quenching of intrinsic emission of enzyme. The thermodynamic data suggest that hydrophobic interactions play a major role in the binding reaction. UV-vis spectroscopy results represented the changes in tryptophan (Trp) absorption and Soret band spectra, which indicated changes in Trp and heme group position caused by the drug binding. Also, CD data represented that high concentrations of DFX lead to a significant decreasing in the content of β-sheet and random coil accompanied an increasing in α-helical content of the protein. The molecular docking results indicate that docking may be an appropriate method for prediction and confirmation of experimental results and also useful for determining the binding mechanism of proteins and drugs. According to above results, it can be concluded that the DFX can chelate the Fe(III) on the enzyme active site leading
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Einmahl, J.H.J.; Gantner, M.; Sawitzki, G.
2008-01-01
The shorth plot is a tool to investigate probability mass concentration. It is a graphical representation of the length of the shorth, the shortest interval covering a certain fraction of the distribution, localized by forcing the intervals considered to contain a given point x. It is easy to
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Variability of Measured Runoff and Soil Loss from Field Plots
Directory of Open Access Journals (Sweden)
F. Asadzadeh
2016-02-01
Full Text Available Introduction: Field plots are widely used in studies related to the measurements of soil loss and modeling of erosion processes. Research efforts are needed to investigate factors affecting the data quality of plots. Spatial scale or size of plots is one of these factors which directly affects measuring runoff and soil loss by means of field plots. The effect of plot size on measured runoff or soil loss from natural plots is known as plot scale effect. On the other hand, variability of runoff and sediment yield from replicated filed plots is a main source of uncertainty in measurement of erosion from plots which should be considered in plot data interpretation processes. Therefore, there is a demand for knowledge of soil erosion processes occurring in plots of different sizes and of factors that determine natural variability, as a basis for obtaining soil loss data of good quality. This study was carried out to investigate the combined effects of these two factors by measurement of runoff and soil loss from replicated plots with different sizes. Materials and Methods: In order to evaluate the variability of runoff and soil loss data seven plots, differing in width and length, were constructed in a uniform slope of 9% at three replicates at Koohin Research Station in Qazvin province. The plots were ploughed up to down slope in September 2011. Each plot was isolated using soil beds with a height of 30 cm, to direct generated surface runoff to the lower part of the plots. Runoff collecting systems composed of gutters, pipes and tankswere installed at the end of each plot. During the two-year study period of 2011-2012, plots were maintained in bare conditions and runoff and soil loss were measured for each single event. Precipitation amounts and characteristics were directly measured by an automatic recording tipping-bucket rain gauge located about 200 m from the experimental plots. The entire runoff volume including eroded sediment was measured on
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Aquifer test interpretation using derivative analysis and diagnostic plots
Hernández-Espriú, Antonio; Real-Rangel, Roberto; Cortés-Salazar, Iván; Castro-Herrera, Israel; Luna-Izazaga, Gabriela; Sánchez-León, Emilio
2017-04-01
Pumping tests remain a method of choice to deduce fundamental aquifer properties and to assess well condition. In the oil and gas (O&G) industry, well testing has been the core technique in examining reservoir behavior over the last 50 years. The pressure derivative by Bourdet, it is perhaps, the most significant single development in the history of well test analysis. Recently, the so-called diagnostics plots (e.g. drawdown and drawdown derivative in a log-log plot) have been successfully tested in aquifers. However, this procedure is still underutilized by groundwater professionals. This research illustrates the applicability range, advantages and drawbacks (e.g. smoothing procedures) of diagnostic plots using field examples from a wide spectrum of tests (short/long tests, constant/variable flow rates, drawdown/buildup stages, pumping well/observation well) in dissimilar geological conditions. We analyze new and pre-existent aquifer tests in Mexico, USA, Canada, Germany, France and Saudi Arabia. In constant flow rate tests, our results show that derivative analysis is an easy, robust and powerful tool to assess near-borehole damage effects, formation heterogeneity, boundaries, flow regimes, infinite-acting radial stages, i.e., valid Theisian framework, and fracture-driven flow. In step tests, the effectiveness relies on high-frequency drawdown measurements. Moreover, we adapt O&G analytical solutions to cater for the conditions in groundwater systems. In this context, further parameters can be computed analytically from the plots, such as skin factor, head losses, wellbore storage, distance to the boundary, channel-aquifer and/or fracture zone width, among others. Therefore, diagnostic plots should be considered a mandatory tool for pumping tests analysis among hydrogeologists. This project has been supported by DGAPA (UNAM) under the research project PAPIIT IN-112815.
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ABCASH plotting program users guide
International Nuclear Information System (INIS)
Troyer, G.L.
1995-01-01
The Automated Bar Coding of Air Samples at Hanford (ABCASH) system provides an integrated data collection, sample tracking, and data reporting system for radioactive particulate air filter samples. The ABCASH plotting program provides a graphical trend report for ABCASH of the performance of air sample results. This document provides an operational guide for using the program. Based on sample location identifier and date range, a trend chart of the available data is generated. The trend chart shows radiological activity versus time. General indications of directional trend of the concentrations in air over time may be discerned. Comparison limit set point values are also shown as derived from the ABCASH data base
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Genome U-Plot: a whole genome visualization.
Gaitatzes, Athanasios; Johnson, Sarah H; Smadbeck, James B; Vasmatzis, George
2018-05-15
The ability to produce and analyze whole genome sequencing (WGS) data from samples with structural variations (SV) generated the need to visualize such abnormalities in simplified plots. Conventional two-dimensional representations of WGS data frequently use either circular or linear layouts. There are several diverse advantages regarding both these representations, but their major disadvantage is that they do not use the two-dimensional space very efficiently. We propose a layout, termed the Genome U-Plot, which spreads the chromosomes on a two-dimensional surface and essentially quadruples the spatial resolution. We present the Genome U-Plot for producing clear and intuitive graphs that allows researchers to generate novel insights and hypotheses by visualizing SVs such as deletions, amplifications, and chromoanagenesis events. The main features of the Genome U-Plot are its layered layout, its high spatial resolution and its improved aesthetic qualities. We compare conventional visualization schemas with the Genome U-Plot using visualization metrics such as number of line crossings and crossing angle resolution measures. Based on our metrics, we improve the readability of the resulting graph by at least 2-fold, making apparent important features and making it easy to identify important genomic changes. A whole genome visualization tool with high spatial resolution and improved aesthetic qualities. An implementation and documentation of the Genome U-Plot is publicly available at https://github.com/gaitat/GenomeUPlot. vasmatzis.george@mayo.edu. Supplementary data are available at Bioinformatics online.
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Rose, Brian
1979-01-01
Examines the content of daytime serial dramas to determine how the narrative structure promotes a sense of involvement in viewers. Competing plot lines, the lack of a concrete sense of resolution, the pattern of episodes, and the audience's awareness of information kept secret from characters all contribute to audience involvement. (JMF)
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Estimation of Missing Observations in Two-Level Split-Plot Designs
DEFF Research Database (Denmark)
Almimi, Ashraf A.; Kulahci, Murat; Montgomery, Douglas C.
2008-01-01
Inserting estimates for the missing observations from split-plot designs restores their balanced or orthogonal structure and alleviates the difficulties in the statistical analysis. In this article, we extend a method due to Draper and Stoneman to estimate the missing observations from unreplicated...... two-level factorial and fractional factorial split-plot (FSP and FFSP) designs. The missing observations, which can either be from the same whole plot, from different whole plots, or comprise entire whole plots, are estimated by equating to zero a number of specific contrast columns equal...... to the number of the missing observations. These estimates are inserted into the design table and the estimates for the remaining effects (or alias chains of effects as the case with FFSP designs) are plotted on two half-normal plots: one for the whole-plot effects and the other for the subplot effects...
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Robert E. Keane
2006-01-01
The Plot Description (PD) form is used to describe general characteristics of the FIREMON macroplot to provide ecological context for data analyses. The PD data characterize the topographical setting, geographic reference point, general plant composition and cover, ground cover, fuels, and soils information. This method provides the general ecological data that can be...
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Validity of the t-plot method to assess microporosity in hierarchical micro/mesoporous materials.
Galarneau, Anne; Villemot, François; Rodriguez, Jeremy; Fajula, François; Coasne, Benoit
2014-11-11
The t-plot method is a well-known technique which allows determining the micro- and/or mesoporous volumes and the specific surface area of a sample by comparison with a reference adsorption isotherm of a nonporous material having the same surface chemistry. In this paper, the validity of the t-plot method is discussed in the case of hierarchical porous materials exhibiting both micro- and mesoporosities. Different hierarchical zeolites with MCM-41 type ordered mesoporosity are prepared using pseudomorphic transformation. For comparison, we also consider simple mechanical mixtures of microporous and mesoporous materials. We first show an intrinsic failure of the t-plot method; this method does not describe the fact that, for a given surface chemistry and pressure, the thickness of the film adsorbed in micropores or small mesopores (plot method to estimate the micro- and mesoporous volumes of hierarchical samples is then discussed, and an abacus is given to correct the underestimated microporous volume by the t-plot method.
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Split-plot designs for multistage experimentation
DEFF Research Database (Denmark)
Kulahci, Murat; Tyssedal, John
2016-01-01
at the same time will be more efficient. However, there have been only a few attempts in the literature to provide an adequate and easy-to-use approach for this problem. In this paper, we present a novel methodology for constructing two-level split-plot and multistage experiments. The methodology is based...... be accommodated in each stage. Furthermore, split-plot designs for multistage experiments with good projective properties are also provided....
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International Nuclear Information System (INIS)
Zhang Zhaohong; Xu Danping; Tie Mei; Li Fangyi; Chen Zhonglin; Wang Jie; Gao Wei; Ji Xiaotong; Xu Yao
2011-01-01
In this study, the interaction between bisphenol A (BPA) or its degraded solution under microwave irradiation after their adsorption on activated carbon (AC/MW) and human serum albumin (HSA) was investigated by UV-vis and fluorescence spectroscopy techniques. The results showed that BPA could bind to HSA molecule, which could cause the stretch of peptide chains. Also, the degraded BPA solution with a few residues could still interact with HSA. Otherwise, the influences of pH and ionic strength on the interaction were estimated. The fluorescence quenching modes of HSA initiated by BPA at three temperatures (298, 310 and 315 K) were all obtained using Stern-Volmer and Lineweaver-Burk equations. The number of binding sites (n), binding constants (K D ) and energy transfer efficiency (E) were all calculated. The thermodynamic parameters (ΔH, ΔG and ΔS) and binding distances (r) were all measured at the three temperatures, respectively. Synchronous fluorescence spectroscopy was also carried out. - Highlights: →The interaction between bisphenol A (BPA) and human serum albumin (HSA) was investigated. → The interaction between degraded BPA solution and HSA was also studied. → The fluorescence quenching mode of HSA initiated by BPA was obtained. → The number of binding site (n) and binding constant (K D ) and their binding distances (r) between BPA and HSA were calculated.
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Theoretical investigation on structural and electronic properties of PdO{sub 2}
Energy Technology Data Exchange (ETDEWEB)
Viswanathan, E.; Sundareswari, M., E-mail: sund-uday@yahoo.co.in, E-mail: sundare65@gmail.com; Jayalakshmi, D. S.; Manjula, M. [Department of Physics, Sathyabama University, Jeppiaar Nagar, OMR, Chennai-600119 (India)
2015-06-24
Theoretical studies on rutile type Palladium Dioxide were carried out with the aim of analyzing structural and electronic properties at ambient condition using the first principle calculation based on density functional theory. Within the framework of density functional theory, we used full potential linearized augmented plane wave method(FP-LAPW) in Wien 2k code. The exchange and correlation effect is treated with generalized gradient approximation (GGA) using the Perdew, Burke and Eruzeroff form. The charge density plots, density of states and band structure are plotted and discussed.
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SUPERIMPOSED MESH PLOTTING IN MCNP
Energy Technology Data Exchange (ETDEWEB)
J. HENDRICKS
2001-02-01
The capability to plot superimposed meshes has been added to MCNP{trademark}. MCNP4C featured a superimposed mesh weight window generator which enabled users to set up geometries without having to subdivide geometric cells for variance reduction. The variance reduction was performed with weight windows on a rectangular or cylindrical mesh superimposed over the physical geometry. Experience with the new capability was favorable but also indicated that a number of enhancements would be very beneficial, particularly a means of visualizing the mesh and its values. The mathematics for plotting the mesh and its values is described here along with a description of other upgrades.
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SplicePlot: a utility for visualizing splicing quantitative trait loci.
Wu, Eric; Nance, Tracy; Montgomery, Stephen B
2014-04-01
RNA sequencing has provided unprecedented resolution of alternative splicing and splicing quantitative trait loci (sQTL). However, there are few tools available for visualizing the genotype-dependent effects of splicing at a population level. SplicePlot is a simple command line utility that produces intuitive visualization of sQTLs and their effects. SplicePlot takes mapped RNA sequencing reads in BAM format and genotype data in VCF format as input and outputs publication-quality Sashimi plots, hive plots and structure plots, enabling better investigation and understanding of the role of genetics on alternative splicing and transcript structure. Source code and detailed documentation are available at http://montgomerylab.stanford.edu/spliceplot/index.html under Resources and at Github. SplicePlot is implemented in Python and is supported on Linux and Mac OS. A VirtualBox virtual machine running Ubuntu with SplicePlot already installed is also available.
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Reaction Order Ambiguity in Integrated Rate Plots
Lee, Joe
2008-01-01
Integrated rate plots are frequently used in reaction kinetics to determine orders of reactions. It is often emphasised, when using this methodology in practice, that it is necessary to monitor the reaction to a substantial fraction of completion for these plots to yield unambiguous orders. The present article gives a theoretical and statistical…
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Hard magnetic property and δM(H) plot for sintered NdFeB magnet
International Nuclear Information System (INIS)
Gao, R.W.; Zhang, D.H.; Li, W.; Li, X.M.; Zhang, J.C.
2000-01-01
The hard magnetic properties and the interactions between the grains for sintered Nd 16 Fe 73 Co 5 B 6 magnets are investigated by using δM(H) plot technique. The results show that the δM(H) plot of NdFeB sintered magnet can explain the effects of the microstructure (size, shape and orientation of the grains) and the intergrain interactions on the hard magnetic properties of the magnet. However, the value of δM(H) is positive when the applied field is not strong enough, which means that the common δM(H) plot theory is not completely consistent with the sintered NdFeB magnet
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Contemplation on Plot and Personification in Tehran Makhuf
Mirjalil Akrami; Mohammad Pashaei
2013-01-01
Abstract Story is one of the important and influential literary genres that deserves to research on its structure and content .By study and analysis of a story, the reader could comprehend the textual messages and change his attitude toward life and different issues .After defining of the social novel, the author tries to analyze plot and personification in novel of “Tehran-e- Makhuf” and respond to the questions on the method employed for plot and personification and analyze it from plot...
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PET kinetic analysis --pitfalls and a solution for the Logan plot.
Kimura, Yuichi; Naganawa, Mika; Shidahara, Miho; Ikoma, Yoko; Watabe, Hiroshi
2007-01-01
The Logan plot is a widely used algorithm for the quantitative analysis of neuroreceptors using PET because it is easy to use and simple to implement. The Logan plot is also suitable for receptor imaging because its algorithm is fast. However, use of the Logan plot, and interpretation of the formed receptor images should be regarded with caution, because noise in PET data causes bias in the Logan plot estimates. In this paper, we describe the basic concept of the Logan plot in detail and introduce three algorithms for the Logan plot. By comparing these algorithms, we demonstrate the pitfalls of the Logan plot and discuss the solution.
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Pan, Yunping; Zhang, Fangfang; Liu, Ru; Jing, Yan; Shen, Jihong; Li, Zhongjian; Zhu, Huaijie
2014-06-01
To explore the characteristics of RR-Lorenz plot in persistent atrial fibrillation (AF) patients complicating with escape beats and rhythm though ambulatory electrocardiogram. The 24-hour ambulatory electrocardiogram of 291 persistent AF patients in second affiliated hospital of Zhengzhou university from July 2005 to April 2013 were retrospectively analyzed and the RR interval and the QRS wave were measured. Patients were divided into two groups according to the distribution of the RR-Lorenz point [AF without escape beats and rhythm group (Group A, n = 259) and AF with escape beats and rhythm group (Group B, n = 32)]. The characteristics of RR-Lorenz plot between the two groups were compared. (1) Fan-shaped RR-Lorenz plots were evidenced in Group A. (2)In Group B, 30 cases showed fan-shaped with L-shaped and a short dense rods along 45° line. The proportion of escape beats and rhythm was 0.28% (275/98 369) -14.06% (11 263/80 112) . The other 2 cases in group B showed no typical RR-Lorenz plots features. RR-Lorenz plot could help to quickly diagnose persistent AF complicating with escape beats and rhythm according to the typical RR-Lorenz plot characteristics in 24-hour ambulatory electrocardiogram.
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SCALPLO, Plotting of Flux Output from SCALE Program
International Nuclear Information System (INIS)
Hersman, A.; De Leege, P.F.A.; Hoogenboom, J.E.
1993-01-01
1 - Description of program or function: SCALPLO is a plot program, designed to plot flux, power and spectrum information. Data exchange between SCALE modules and SCALPLO is via CCCC-interface files. As not all modules can produce these files, there are special routines supplied with SCALPLO that can produce CCCC-like files. These routines can be included in the code and for XSDRPM, CITATION, ANISN and DOT, the place to include these routines is supplied. 2 - Method of solution: SCALPLO consists of two sections. Firstly the pre-processor, which selects and reads the required data. Secondly the plot section which produces the plot on the selected output device. 3 - Restrictions on the complexity of the problem: SCALPLO requires DISSPLA version 11.0 or higher. The choice of output device depends on the devices installed
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Development of TRatioPlot in ROOT
Gessinger-Befurt, Paul
2016-01-01
The ROOT data analysis and visualization framework is a software package which is widely used in physics, especially in high energy physics. A common visualization which has so far been lacking a direct implementation is the ratio plot, as well as a few similar types of plots. The scope and goal of the summer student project at CERN was to implement a class in ROOT itself, that can take care of the most common types of calculations, and produces high quality visuals.
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Digital data collection in forest dynamics plots
Faith Inman-Narahari; Christian Giardina; Rebecca Ostertag; Susan Cordell; Lawren Sack
2010-01-01
Summary 1. Computers are widely used in all aspects of research but their application to in-field data collection for forest plots has rarely been evaluated. 2. We developed digital data collection methods using ESRI mapping software and ruggedized field computers to map and measure ~30 000 trees in two 4-ha forest dynamics plots in wet and dry...
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Longleaf pine regeneration following Hurricane Ivan utilizing the RLGS plots
John C. Gilbert; John S. Kush
2013-01-01
On September 16, 2004, Hurricane Ivan hit the Alabama coast and severely impacted numerous plots in the U.S. Forest Serviceâs Regional Longleaf Growth Study (RLGS). The Escambia Experimental Forest (EEF) has 201 of the 325 RLGS plots. Nearly one-third of the EEF was impacted. Nine plots with pole-sized trees were entirely lost. Another 54 plots had some type of damage...
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SEGY to ASCII: Conversion and Plotting Program
Goldman, Mark R.
1999-01-01
This report documents a computer program to convert standard 4 byte, IBM floating point SEGY files to ASCII xyz format. The program then optionally plots the seismic data using the GMT plotting package. The material for this publication is contained in a standard tar file (of99-126.tar) that is uncompressed and 726 K in size. It can be downloaded by any Unix machine. Move the tar file to the directory you wish to use it in, then type 'tar xvf of99-126.tar' The archive files (and diskette) contain a NOTE file, a README file, a version-history file, source code, a makefile for easy compilation, and an ASCII version of the documentation. The archive files (and diskette) also contain example test files, including a typical SEGY file along with the resulting ASCII xyz and postscript files. Requirements for compiling the source code into an executable are a C++ compiler. The program has been successfully compiled using Gnu's g++ version 2.8.1, and use of other compilers may require modifications to the existing source code. The g++ compiler is a free, high quality C++ compiler and may be downloaded from the ftp site: ftp://ftp.gnu.org/gnu Requirements for plotting the seismic data is the existence of the GMT plotting package. The GMT plotting package may be downloaded from the web site: http://www.soest.hawaii.edu/gmt/
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True versus perturbed forest inventory plot locations for modeling: a simulation study
John W. Coulston; Kurt H. Riitters; Ronald E. McRoberts; William D. Smith
2006-01-01
USDA Forest Service Forest Inventory and Analysis plot information is widely used for timber inventories, forest health assessments, and environmental risk analyses. With few exceptions, true plot locations are not revealed; the plot coordinates are manipulated to obscure the location of field plots and thereby preserve plot integrity. The influence of perturbed plot...
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FLOWCHART; a computer program for plotting flowcharts
Bender, Bernice
1982-01-01
The computer program FLOWCHART can be used to very quickly and easily produce flowcharts of high quality for publication. FLOWCHART centers each element or block of text that it processes on one of a set of (imaginary) vertical lines. It can enclose a text block in a rectangle, circle or other selected figure. It can draw a 'line connecting the midpoint of any side of any figure with the midpoint of any side of any other figure and insert an arrow pointing in the direction of flow. It can write 'yes' or 'no' next to the line joining two figures. FLOWCHART creates flowcharts using some basic plotting subroutine* which permit plots to be generated interactively and inspected on a Tektronix compatible graphics screen or plotted in a deferred mode on a Houston Instruments 42' pen plotter. The size of the plot, character set and character height in inches are inputs to the program. Plots generated using the pen plotter can be up to 42' high--the larger size plots being directly usable as visual aids in a talk. FLOWCHART centers each block of text on an imaginary column line. (The number of columns and column width are specified as input.) The midpoint of the longest line of text within the block is defined to be the center of the block and is placed on the column line. The spacing of individual words within the block is not altered when the block is positioned. The program writes the first block of text in a designated column and continues placing each subsequent block below the previous block in the same column. A block of text may be placed in a different column by specifying the number of the column and an earlier block of text with which the new block is to be aligned. If block zero is given as the earlier block, the new text is placed in the new column continuing down the page below the previous block. Optionally a column and number of inches from the top of the page may be given for positioning the next block of text. The program will normally draw one of five
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Directory of Open Access Journals (Sweden)
Raquel Kritsch
2011-08-01
Full Text Available
O objetivo deste artigo é introduzir ao leitor o pensamento político de Edmund Burke, um dos nomes mais influentes do pensamento político conservador. A partir de uma breve contextualização da crítica burkeana ao racionalismo iluminista, o artigo procura mostrar como o autor organiza conceitos centrais de sua teoria política, como poder, legitimidade, conservação, correção, preconceito, prescrição, num discurso articulado e coerente, com o objetivo de sustentar que na Inglaterra política e religião constituem, juntas, não só a base não só da prosperidade do povo mas também o fundamento do Estado. Em seguida, aborda-se a crítica de Burke à Revolução Francesa, à “psicologia dos revolucionários” e, de maneira mais detida, aos direitos do homem, discutindo alguns de seus argumentos contra as abstrações distanciadas das práticas concretas dos coletivos humanos e a transformação da metafísica moderna num programa político.
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This tool plots daily AQI values for a specific location and time period. Each square or “tile” represents one day of the year and is color-coded based on the AQI level for that day. The legend tallies the number of days in each AQI category.
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Vandegriff, J. D.; Kusterer, M. B.; Byun, S.; Steele, R. J.; Mitchell, D. G.
2017-12-01
We present a new mobile app for Android and an existing app for iPhone, both capable of viewing the numerous browse plots available for data collected by the MIMI suite on NASA's Cassini spacecraft. Both apps allow convenient mobile access to pre-made plots of data from various instruments on the suite, including daily, and monthly plots of particle intensities (line plots and spectrograms) from LEMMS, CHEMS and INCA. Also, the apps can show short movies made from sequences of INCA neutral atom images. Browsing the plots or movies is as simple as swiping to the left or right, and the app hides all access details needed to finding the images. Note that the app requires a data connection, since it locates and downloads the plot files live from various instrument team servers. We will demonstrate the current versions of both apps, which are available in Apple's App Store and the Google Play Store.
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[Eugenics, an element of the literary plots of dystopia].
Baum, Ewa; Musielak, Michał
2007-01-01
The work presents the ideas and assumptions of eugenics, a social philosophy established in 1883 by Francis Galton, which affected the social policies of numerous European countries in the first half of the 20th century. The work shows the effect of eugenics on the literary standards of European prose in the previous century. Two outstanding dystopian novels of the 20th century, The Brave New World by Aldous Huxley and 1984 by George Orwell, situate eugenics as a permanent element of the literary plot of dystopia. Apart from the typical features of this type of novel, for example: personal narration with a trace of irony, a totalitarian state and Newspeak, eugenics is an important element of the literary plot with is aim to exclude and marginalise certain social groups. Eugenics is also one of the main social ideas criticised by both the writers.
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SACCHARIFICATION OF NATIVE CASSAVA STARCH AT HIGH DRY SOLIDS IN AN ENZYMATIC MEMBRANE REACTOR
Directory of Open Access Journals (Sweden)
I Nyoman Widiasa
2012-02-01
Full Text Available This study is aimed to develop a novel process scheme for hydrolysis of native cassava starch at high dry solids using an enzymatic membrane reactor (EMR. Firstly, liquefied cassava starch having solids content up to 50% by weight was prepared by three stage liquefactions in a conventional equipment using a commercially available heat stable a-amylase (Termamyl 120L. The liquefied cassava starch was further saccharified in an EMR using glucoamylase (AMG E. By using the developed process scheme, a highly clear hydrolysate with dextrose equivalent (DE approximately 97 could be produced, provided the increase of solution viscosity during the liquefaction was precisely controlled. The excessive space time could result in reduction in conversion degree of starch. Moreover, a residence time distribution study confirmed that the EMR could be modelled as a simple continuous stirred tank reactor (CSTR. Using Lineweaver-Burk analysis, the apparent Michaelis-Menten constant (Km and glucose production rate constant (k2 were 552 (g/l and 4.04 (min-1, respectively. Application of simple CSTR model with those kinetic parameters was quietly appropriate to predict the reactor’s performance at low space time.
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Ascorbic acid transport and accumulation in human neutrophils
International Nuclear Information System (INIS)
Washko, P.; Rotrosen, D.; Levine, M.
1989-01-01
The transport, accumulation, and distribution of ascorbic acid were investigated in isolated human neutrophils utilizing a new ascorbic acid assay, which combined the techniques of high performance liquid chromatography and coulometric electrochemical detection. Freshly isolated human neutrophils contained 1.0-1.4 mM ascorbic acid, which was localized greater than or equal to 94% to the cytosol, was not protein bound, and was present only as ascorbic acid and not as dehydroascorbic acid. Upon addition of ascorbic acid to the extracellular medium in physiologic amounts, ascorbic acid was accumulated in neutrophils in millimolar concentrations. Accumulation was mediated by a high affinity and a low affinity transporter; both transporters were responsible for maintenance of concentration gradients as large as 50-fold. The high affinity transporter had an apparent Km of 2-5 microns by Lineweaver-Burk and Eadie-Hofstee analyses, and the low affinity transporter had an apparent Km of 6-7 mM by similar analyses. Each transporter was saturable and temperature dependent. In normal human blood the high affinity transporter should be saturated, whereas the low affinity transporter should be in its linear phase of uptake
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Köksal, Ekrem; Gülçin, Ilhami
2008-01-01
Peroxidases (EC 1.11.1.7; donor: hydrogen peroxide oxidoreductase) are part of a large group of enzymes. In this study, peroxidase, a primer antioxidant enzyme, was purified with 19.3 fold and 0.2% efficiency from cauliflower (Brassica oleracea L.) by ammonium sulphate precipitation, dialysis, CM-Sephadex ion-exchange chromatography and Sephadex G-25 purification steps. The substrate specificity of peroxidase was investigated using 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulphonic acid) (ABTS), 2-methoxyphenol (guaiacol), 1,2-dihydroxybenzene (catechol), 1,2,3-trihyidroxybenzene (pyrogallol) and 4-methylcatechol. Also, optimum pH, optimum temperature, optimum ionic strength, stable pH, stable temperature, thermal inactivation conditions were determined for guaiacol/H(2)O(2), pyrogallol/H(2)O(2), ABTS/H(2)O(2), catechol/H(2)O(2) and 4-methyl catechol/H(2)O(2) substrate patterns. The molecular weight (M(w)) of this enzyme was found to be 44 kDa by gel filtration chromatography method. Native polyacrylamide gel electrophoresis (PAGE) was performed for isoenzyme determination and a single band was observed. K(m) and V(max) values were calculated from Lineweaver-Burk graph for each substrate patterns.
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PLOT3D Export Tool for Tecplot
Alter, Stephen
2010-01-01
The PLOT3D export tool for Tecplot solves the problem of modified data being impossible to output for use by another computational science solver. The PLOT3D Exporter add-on enables the use of the most commonly available visualization tools to engineers for output of a standard format. The exportation of PLOT3D data from Tecplot has far reaching effects because it allows for grid and solution manipulation within a graphical user interface (GUI) that is easily customized with macro language-based and user-developed GUIs. The add-on also enables the use of Tecplot as an interpolation tool for solution conversion between different grids of different types. This one add-on enhances the functionality of Tecplot so significantly, it offers the ability to incorporate Tecplot into a general suite of tools for computational science applications as a 3D graphics engine for visualization of all data. Within the PLOT3D Export Add-on are several functions that enhance the operations and effectiveness of the add-on. Unlike Tecplot output functions, the PLOT3D Export Add-on enables the use of the zone selection dialog in Tecplot to choose which zones are to be written by offering three distinct options - output of active, inactive, or all zones (grid blocks). As the user modifies the zones to output with the zone selection dialog, the zones to be written are similarly updated. This enables the use of Tecplot to create multiple configurations of a geometry being analyzed. For example, if an aircraft is loaded with multiple deflections of flaps, by activating and deactivating different zones for a specific flap setting, new specific configurations of that aircraft can be easily generated by only writing out specific zones. Thus, if ten flap settings are loaded into Tecplot, the PLOT3D Export software can output ten different configurations, one for each flap setting.
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On the curvature of transmitted intensity plots in broad beam studies
International Nuclear Information System (INIS)
El-Kateb, A.H.
2000-01-01
Transmission of a broad beam of gamma rays of 81- and 356-keV energies from 133 Ba is studied singly and dually. This study is the first to deal with the curvatures of the intensity plots. The targets are dextrose solutions of percentage concentrations up to 0.125 and soil containing water with concentrations up to 0.319. The logarithmic intensity plots are expressed in terms of a polynomial in the concentration. The curvatures of the plots are measured and calculated on the basis of the theoretical mass attenuation coefficients. The results are discussed in conjunction with buildup factors and the probability of photoelectric and Compton interactions. The curvatures show maxima when incoherent interaction prevails. This is evidently proved in case of the single 356-keV and of the dual 81- and 356-keV applied energies. Comparison is performed between the measured and calculated curvatures. The concept of curvature is applied and discussed for published results of narrow beam geometry. Correspondingly, this is the first search to introduce curvature instead of buildup as a measure for transmitted collided photons
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PETRO.CALC.PLOT, Microsoft Excel macros to aid petrologic interpretation
Sidder, G.B.
1994-01-01
PETRO.CALC.PLOT is a package of macros which normalizes whole-rock oxide data to 100%, calculates the cation percentages and molecular proportions used for normative mineral calculations, computes the apices for ternary diagrams, determines sums and ratios of specific elements of petrologic interest, and plots 33 X-Y graphs and five ternary diagrams. PETRO.CALC.PLOT also may be used to create other diagrams as desired by the user. The macros run in Microsoft Excel 3.0 and 4.0 for Macintosh computers and in Microsoft Excel 3.0 and 4.0 for Windows. Macros provided in PETRO.CALC.PLOT minimize repetition and time required to recalculate and plot whole-rock oxide data for petrologic analysis. ?? 1994.
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Plotting of bathythermograph transect data on a printer
Reynolds, James B.; McLain, Douglas R.
1971-01-01
A program for plotting bathythermograph transect data on a computer (IBM 1130) printer is available from the Great Lakes Fishery Laboratory. Temperature values are printed in positions proportional to their depths and distances from shore. Contour lines are drawn manually through the plotted points.
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Privatization of Land Plot Under Integral Real Estate Complex
Directory of Open Access Journals (Sweden)
Maruchek A. A.
2014-10-01
Full Text Available The article deals with the questions concerning the privatization of a land plot under integral real estate complex. The authors come to conclusion that a number of legislation norms relating to privatization of a land plot do not take into account the construction of an integral real estate complex that could cause some problems in the realization of the right to privatization of the land plot
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Plotting and Analyzing Data Trends in Ternary Diagrams Made Easy
John, Cédric M.
2004-04-01
Ternary plots are used in many fields of science to characterize a system based on three components. Triangular plotting is thus useful to a broad audience in the Earth sciences and beyond. Unfortunately, it is typically the most expensive commercial software packages that offer the option to plot data in ternary diagrams, and they lack features that are paramount to the geosciences, such as the ability to plot data directly into a standardized diagram and the possibility to analyze temporal and stratigraphic trends within this diagram. To address these issues, δPlot was developed with a strong emphasis on ease of use, community orientation, and availability free of charges. This ``freeware'' supports a fully graphical user interface where data can be imported as text files, or by copying and pasting. A plot is automatically generated, and any standard diagram can be selected for plotting in the background using a simple pull-down menu. Standard diagrams are stored in an external database of PDF files that currently holds some 30 diagrams that deal with different fields of the Earth sciences. Using any drawing software supporting PDF, one can easily produce new standard diagrams to be used with δPlot by simply adding them to the library folder. An independent column of values, commonly stratigraphic depths or ages, can be used to sort the data sets.
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Slope Stability of Geosynthetic Clay Liner Test Plots
Fourteen full-scale field test plots containing five types of geosynthetic clay liners (GCLs) were constructed on 2H:IV and 3H:IV slopes for the purpose of assessing slope stability. The test plots were designed to simulate typical final cover systems for landfill. Slides occurr...
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Feng, S.; Heerink, N.; Ruben, R.; Qu, F.
2010-01-01
This paper performs a plot-level analysis of the impact of land rental market participation and off-farm employment on land investment, input use, and rice yields for 215 plots cultivated by 52 households in three villages in Northeast Jiangxi Province. Our findings show that households that rent
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Chain Plot: A Tool for Exploiting Bivariate Temporal Structures
Taylor, CC; Zempeni, A
2004-01-01
In this paper we present a graphical tool useful for visualizing the cyclic behaviour of bivariate time series. We investigate its properties and link it to the asymmetry of the two variables concerned. We also suggest adding approximate confidence bounds to the points on the plot and investigate the effect of lagging to the chain plot. We conclude our paper by some standard Fourier analysis, relating and comparing this to the chain plot.
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User manual for two simple postscript output FORTRAN plotting routines
Nguyen, T. X.
1991-01-01
Graphics is one of the important tools in engineering analysis and design. However, plotting routines that generate output on high quality laser printers normally come in graphics packages, which tend to be expensive and system dependent. These factors become important for small computer systems or desktop computers, especially when only some form of a simple plotting routine is sufficient. With the Postscript language becoming popular, there are more and more Postscript laser printers now available. Simple, versatile, low cost plotting routines that can generate output on high quality laser printers are needed and standard FORTRAN language plotting routines using output in Postscript language seems logical. The purpose here is to explain two simple FORTRAN plotting routines that generate output in Postscript language.
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Multilocational evaluation of white yam genotypes using GGE bi-plot ...
African Journals Online (AJOL)
Five new white yam genotypes were evaluated in different locations of major yam producing areas; Umudike, Nsukka, Ubiaja, Abuja and Katsina-Ala, to test the performance and stability of these genotypes across the environments using GGE bi-plot software. The GGE bi-plot generated several graphic bi-plots which ...
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On-plot drinking water supplies and health: A systematic review.
Overbo, Alycia; Williams, Ashley R; Evans, Barbara; Hunter, Paul R; Bartram, Jamie
2016-07-01
Many studies have found that household access to water supplies near or within the household plot can reduce the probability of diarrhea, trachoma, and other water-related diseases, and it is generally accepted that on-plot water supplies produce health benefits for households. However, the body of research literature has not been analyzed to weigh the evidence supporting this. A systematic review was conducted to investigate the impacts of on-plot water supplies on diarrhea, trachoma, child growth, and water-related diseases, to further examine the relationship between household health and distance to water source and to assess whether on-plot water supplies generate health gains for households. Studies provide evidence that households with on-plot water supplies experience fewer diarrheal and helminth infections and greater child height. Findings suggest that water-washed (hygiene associated) diseases are more strongly impacted by on-plot water access than waterborne diseases. Few studies analyzed the effects of on-plot water access on quantity of domestic water used, hygiene behavior, and use of multiple water sources, and the lack of evidence for these relationships reveals an important gap in current literature. The review findings indicate that on-plot water access is a useful health indicator and benchmark for the progressive realization of the Sustainable Development Goal target of universal safe water access as well as the human right to safe water. Copyright © 2016 Elsevier GmbH. All rights reserved.
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6th International Symposium on Recurrence Plots
Jr, Jr; Ioana, Cornel; Marwan, Norbert
2016-01-01
The chapters in this book originate from the research work and contributions presented at the Sixth International Symposium on Recurrence Plots held in Grenoble, France in June 2015. Scientists from numerous disciplines gathered to exchange knowledge on recent applications and developments in recurrence plots and recurrence quantification analysis. This meeting was remarkable because of the obvious expansion of recurrence strategies (theory) and applications (practice) into ever-broadening fields of science. It discusses real-world systems from various fields, including mathematics, strange attractors, applied physics, physiology, medicine, environmental and earth sciences, as well as psychology and linguistics. Even readers not actively researching any of these particular systems will benefit from discovering how other scientists are finding practical non-linear solutions to specific problems. The book is of interest to an interdisciplinary audience of recurrence plot users and researchers interested in time...
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Rodgers, Joseph Lee; Beasley, William Howard; Schuelke, Matthew
2014-01-01
Many data structures, particularly time series data, are naturally seasonal, cyclical, or otherwise circular. Past graphical methods for time series have focused on linear plots. In this article, we move graphical analysis onto the circle. We focus on 2 particular methods, one old and one new. Rose diagrams are circular histograms and can be produced in several different forms using the RRose software system. In addition, we propose, develop, illustrate, and provide software support for a new circular graphical method, called Wrap-Around Time Series Plots (WATS Plots), which is a graphical method useful to support time series analyses in general but in particular in relation to interrupted time series designs. We illustrate the use of WATS Plots with an interrupted time series design evaluating the effect of the Oklahoma City bombing on birthrates in Oklahoma County during the 10 years surrounding the bombing of the Murrah Building in Oklahoma City. We compare WATS Plots with linear time series representations and overlay them with smoothing and error bands. Each method is shown to have advantages in relation to the other; in our example, the WATS Plots more clearly show the existence and effect size of the fertility differential.
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Directory of Open Access Journals (Sweden)
Jose de Jesus Navar Chaidez
2016-05-01
Full Text Available Aboveground tree biomass (bole, branches and foliage, M, plays a key role in the conventional and sustainable management of forest communities. The standard approach to assess tree or plot M is harvesting trees, developing and fitting allometric equations to trees or forest inventory plot data. In the absence of local tree allometry, it is usually recommended to fit off site allometric equations to evaluate tree or plot M. This research aims: (a to develop an updated on site allometric equation (b to fit available off site allometric equations to destructively harvested trees and (c to fit available allometric equations to plot M of Mexico’s Sinaloan tropical dry forests to understand sources of inherent tree and plot M variability. Results showed that: (a the improved on site allometric equation increases precision in contrast to the conventional biomass equation previously reported as well as to off site tree M equations, (b off site allometry projects tree and plot M deviates by close to one order of magnitude. Two tested and recommended approaches to increase tree and plot M precision when fitting off site equations are: (i to use all available tree allometric functions to come up with a mean equation or (ii to calibrate off site equations by fitting new, local parameters that can be calculated using statistical programs.These options would eventually increase tree and plot M precision in regional evaluations.
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arXiv Laura++ : a Dalitz plot fitter
Back, John; Harrison, Paul; Latham, Thomas; O'Hanlon, Daniel; Qian, Wenbin; del Amo Sanchez, Pablo; Craik, Daniel; Ilic, Jelena; Otalora Goicochea, Juan; Puccio, Eugenia; Silva Coutinho, Rafael; Whitehead, Mark
The Dalitz plot analysis technique has become an increasingly important method in heavy flavour physics. The Laura++ fitter has been developed as a flexible tool that can be used for Dalitz plot analyses in different experimental environments. Explicitly designed for three-body decays of heavy-flavoured mesons to spinless final state particles, it is optimised in order to describe all possible resonant or nonresonant contributions, and to accommodate possible CP violation effects.
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Directory of Open Access Journals (Sweden)
Yang Wang
2016-01-01
Full Text Available Testis-specific lactate dehydrogenase (LDH-C4 is one of the lactate dehydrogenase (LDH isozymes that catalyze the terminal reaction of pyruvate to lactate in the glycolytic pathway. LDH-C4 in mammals was previously thought to be expressed only in spermatozoa and testis and not in other tissues. Plateau pika (Ochotona curzoniae belongs to the genus Ochotona of the Ochotonidea family. It is a hypoxia-tolerant species living in remote mountain areas at altitudes of 3000–5000 m above sea level on the Qinghai-Tibet Plateau. Surprisingly, Ldh-c is expressed not only in its testis and sperm, but also in somatic tissues of plateau pika. To shed light on the function of LDH-C4 in somatic cells, Ldh-a, Ldh-b, and Ldh-c of plateau pika were subcloned into bacterial expression vectors. The pure enzymes of Lactate Dehydrogenase A4 (LDH-A4, Lactate Dehydrogenase B4 (LDH-B4, and LDH-C4 were prepared by a series of expression and purification processes, and the three enzymes were identified by the method of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE and native polyacrylamide gel electrophoresis (PAGE. The enzymatic kinetics properties of these enzymes were studied by Lineweaver-Burk double-reciprocal plots. The results showed the Michaelis constant (Km of LDH-C4 for pyruvate and lactate was 0.052 and 4.934 mmol/L, respectively, with an approximate 90 times higher affinity of LDH-C4 for pyruvate than for lactate. At relatively high concentrations of lactate, the inhibition constant (Ki of the LDH isoenzymes varied: LDH-A4 (Ki = 26.900 mmol/L, LDH-B4 (Ki = 23.800 mmol/L, and LDH-C4 (Ki = 65.500 mmol/L. These data suggest that inhibition of lactate by LDH-A4 and LDH-B4 were stronger than LDH-C4. In light of the enzymatic kinetics properties, we suggest that the plateau pika can reduce reliance on oxygen supply and enhance its adaptation to the hypoxic environments due to increased anaerobic glycolysis by LDH-C4.
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International Nuclear Information System (INIS)
Duensing, P.; Jahn, W.; Rehm, W.
1986-09-01
The performance of safety analyses for gas-cooled high temperature reactor power plants requires efficient plot codes for the evaluation and representation of computer results. The report describes the coupling between the thermodynamic simulation code THERMIX and the graphic plot code RAPS via the interface program THERAPIE. Especially the structure and the handling of the interface program are explained as well as the dialogue with the plot code. Further options of the colour graphic system are demonstrated for the representation of temperature distributions in components of HTR concepts (HTR-500). (orig.) [de
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Myth Structure and Media Fiction Plot: An Exploration.
Harless, James D.
Based on the general research of Joseph Campbell in adventure plots from mythology, the author explores the simplified monomyth plots currently in frequent use in mass media programing. The close relationship of media fiction to mythic stories is established through the analysis of more than 25 stories resulting from media broadcasting. The media…
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DEFF Research Database (Denmark)
Edwards, Stefan McKinnon
2012-01-01
In this chapter I will investigate how to combine multiple plots into a single. The scenario is a dataset of a series of measurements, on three samples in three situations. There are many ways we can display this, e.g. 3d graphs or faceting. 3d graphs are not good for displaying static data so we...
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Surveillance of Site A and Plot M
International Nuclear Information System (INIS)
Golchert, N.W.
1993-05-01
The results of the environmental surveillance program conducted at Site A/Plot M in the Palos Forest Preserve area for CY 1992 are presented. The surveillance program is the ongoing remedial action that resulted from the 1976--1978 radiological characterization of the site. That study determined that very low levels of hydrogen-3 (as tritiated water) had migrated from the burial ground and were present in two nearby hand-pumped picnic wells. The current program consists of sample collection and analysis of air, surface and subsurface water, and bottom sediment. The results of the analyses are used to (1) determine the migration pathway of water from the burial ground (Plot M) to the hand-pumped picnic wells, (2) establish if buried radionuclides other than hydrogen-3 have migrated, and (3) generally characterize the radiological environment of the area. Hydrogen-3 in the Red Gate Woods picnic wells was still detected this year, but the average and maximum concentrations were significantly less than found earlier. Tritiated water continues to be detected in a number of wells, boreholes, dolomite holes, and a surface stream. For many years it was the only radionuclide found to have migrated in measurable quantities. Analyses since 1984 have indicated the presence of low levels of strontium-90 in water from a number of boreholes next to Plot M. The available data does not allow a firm conclusion as to whether the presence of this nuclide represents recent migration or movement that may have occurred before Plot M was capped. The results of the surveillance program continue to indicate that the radioactivity remaining at Site A/Plot M does not endanger the health or safety of the public visiting the site, using the picnic area, or living in the vicinity
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Surveillance of Site A and Plot M
International Nuclear Information System (INIS)
Golchert, N.W.
1991-05-01
The results of the environmental surveillance program conducted at Site A/Plot M in the Palos Forest Preserve area for CY 1990 are presented. The surveillance program is the ongoing remedial action that resulted from the 1976-1978 radiological characterization of the site. That study determined that very low levels of hydrogen-3 (as tritiated water) had migrated from the burial ground and were present in two nearby hand-pumped picnic wells. The current program consists of sample collection and analysis of air, surface and subsurface water, and bottom sediment. The results of the analyses are used to determine the migration pathway of water from the burial ground (plot M) to the hand-pumped picnic wells, establish if buries radionuclides other than hydrogen-3 have migrated, and generally characterize the radiological environment of the area. Tritiated water continues to be detected in a number of wells, boreholes, dolomite holes, and a surface stream. Analyses since 1984 have indicated the presence of low levels of strontium-90 in water from a number of boreholes next to Plot M. The results of the surveillance program continues to indicate that the radioactivity remaining at Site A/Plot M does not endanger the health or safety of the public visiting the site or those living in the vicinity. 20 refs., 7 figs., 15 tabs
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The 2002 RPA Plot Summary database users manual
Patrick D. Miles; John S. Vissage; W. Brad Smith
2004-01-01
Describes the structure of the RPA 2002 Plot Summary database and provides information on generating estimates of forest statistics from these data. The RPA 2002 Plot Summary database provides a consistent framework for storing forest inventory data across all ownerships across the entire United States. The data represents the best available data as of October 2001....
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Impact of animal waste application on runoff water quality in field experimental plots.
Hill, Dagne D; Owens, William E; Tchoounwou, Paul B
2005-08-01
Animal waste from dairy and poultry operations is an economical and commonly used fertilizer in the state of Louisiana. The application of animal waste to pasture lands not only is a source of fertilizer, but also allows for a convenient method of waste disposal. The disposal of animal wastes on land is a potential nonpoint source of water degradation. Water degradation and human health is a major concern when considering the disposal of large quantities of animal waste. The objective of this research was to determine the effect of animal waste application on biological (fecal coliform, Enterobacter spp. and Escherichia coli) and physical/chemical (temperature, pH, nitrate nitrogen, ammonia nitrogen, phosphate, copper, zinc, and sulfate) characteristics of runoff water in experimental plots. The effects of the application of animal waste have been evaluated by utilizing experimental plots and simulated rainfall events. Samples of runoff water were collected and analyzed for fecal coliforms. Fecal coliforms isolated from these samples were identified to the species level. Chemical analysis was performed following standard test protocols. An analysis of temperature, ammonia nitrogen, nitrate nitrogen, iron, copper, phosphate, potassium, sulfate, zinc and bacterial levels was performed following standard test protocols as presented in Standard Methods for the Examination of Water and Wastewater [1]. In the experimental plots, less time was required in the tilled broiler litter plots for the measured chemicals to decrease below the initial pre-treatment levels. A decrease of over 50% was noted between the first and second rainfall events for sulfate levels. This decrease was seen after only four simulated rainfall events in tilled broiler litter plots whereas broiler litter plots required eight simulated rainfall events to show this same type of reduction. A reverse trend was seen in the broiler litter plots and the tilled broiler plots for potassium. Bacteria numbers
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Online plot services for paleomagnetism and rock magnetism
Hatakeyama, T.
2017-12-01
In paleomagnetism and rock magnetism, a lot of types of original plots are used for obtained data from measurements. Many researchers in paleomagnetism often use not only general-purpose plotting programs such as Microsoft Excel but also single-purpose tools. A large benefit of using the latter tools is that we can make a beautiful figure for our own data. However, those programs require specific environment for their operation such as type of hardware and platform, type of operation system and its version, libraries for execution and so on. Therefore, it is difficult to share the result and graphics among the collaborators who use different environments on their PCs. Thus, one of the best solution is likely a program operated on popular environment. The most popular is web environment as we all know. Almost all current operating systems have web browsers as standard and all people use them regularly. Now we provide a web-based service plotting paleomagnetic results easily.We develop original programs with a command-line user interface (non-GUI), and we prepared web pages for input of the simple measured data and options and a wrapper script which transfers the entered values to the program. The results, analyzed values and plotted graphs from the program are shown in the HTML page and downloadable. Our plot services are provided in http://mage-p.org/mageplot/. In this talk, we introduce our program and service and discuss the philosophy and efficiency of these services.
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PRP: a FORTRAN IV interactive plotting program
Andrew, A. S.; Linde, J.
A computer program, PRP, has been designed to plot any arithmetic combination selected from a set of major and trace element data on a y- x graph. y and x are defined and entered as a program string (y, x) which is interpreted sequentially. Operators ( +, -, ∗, /, ( unary) , square root, log 10, In c, antilog 10, exponential, integer, absolute value, (,),,) and integer or real numbers may be included. Axis lengths and scales are determined by the user. Five different plotting symbols are available.
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Selection of Plot Remeasurement in an Annual Inventory
Mark H. Hansen; Hans T. Schreuder; Dave Heinzen
2000-01-01
A plot selection approach is proposed based on experience from the Annual Forest Inventory System (AFIS) in the Aspen-Birch Unit of northestern Minnesota. The emphasisis on a mixture of strategies. Although the Agricultural Act of 1998 requires that a fixed 20 percent of plots be measured each year in each state, sooner or later we will need to vary the scheme to...
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Paired split-plot designs of multireader multicase studies.
Chen, Weijie; Gong, Qi; Gallas, Brandon D
2018-07-01
The widely used multireader multicase ROC study design for comparing imaging modalities is the fully crossed (FC) design: every reader reads every case of both modalities. We investigate paired split-plot (PSP) designs that may allow for reduced cost and increased flexibility compared with the FC design. In the PSP design, case images from two modalities are read by the same readers, thereby the readings are paired across modalities. However, within each modality, not every reader reads every case. Instead, both the readers and the cases are partitioned into a fixed number of groups and each group of readers reads its own group of cases-a split-plot design. Using a [Formula: see text]-statistic based variance analysis for AUC (i.e., area under the ROC curve), we show analytically that precision can be gained by the PSP design as compared with the FC design with the same number of readers and readings. Equivalently, we show that the PSP design can achieve the same statistical power as the FC design with a reduced number of readings. The trade-off for the increased precision in the PSP design is the cost of collecting a larger number of truth-verified patient cases than the FC design. This means that one can trade-off between different sources of cost and choose a least burdensome design. We provide a validation study to show the iMRMC software can be reliably used for analyzing data from both FC and PSP designs. Finally, we demonstrate the advantages of the PSP design with a reader study comparing full-field digital mammography with screen-film mammography.
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Surface stabilization and revegetation test plots
International Nuclear Information System (INIS)
Sackschewsky, M.R.; Kemp, C.J.; Hayward, W.M.
1993-09-01
Westinghouse Hanford Company Decommissioning and Decontamination Engineering Group and Environmental Technology and Assessment Groups are developing new technologies to improve revegetation techniques for interim stabilization control over underground waste sites within the Radiation Area Remedial Action Program. Successful revegetation is an integral aspect of waste isolation strategy. Unfortunately, revegetation can be very difficult to achieve on the Hanford Site due to several factors: low annual precipitation, unpredictable timing of precipitation, low fertility of available soils, and coarse physical texture of soils covering waste sites. The tests in this report were performed during fiscal years 1992 and 1993 and include the use of two soil sealants in combination with bare soil and a soil/compost mixture and a comparison of a wheatgrass mixture and a native seed mixture. Hydroprobe access ports were placed in one-half of the test plots and moisture data was collected. Soil fertility and plant community characteristics were monitored during the two years of the test. During the first year all sites with compost provided additional fertility and retained greater amounts of soil moisture than noncomposted sites. The use of Enduraseal soil fixative provided greater soil moisture than the use of Aerospray-77 soil fixative. During the second year the use of compost and soil fixative's had a lesser effect on soil moisture. During late summer periods all treatments had very similar soil moisture profiles. The use of compost greatly increased vegetative cover and soil fertility in comparison to sites that had no compost added. Testing of the seed mixtures found that Siberian wheatgrass and Sandberg's bluegrass were the most dominant of the seeded species observed. All plots exhibited a dominant plant cover of volunteer cheatgrass. Biomass production was significantly greater on plots with compost than on the noncomposted plots
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The Use of Plackett-Burman Designs to Construct Split Plot Designs.
Kulahci, M.; Bisgaard, S.
2005-01-01
Abstract When some factors are hard to change and others are relatively easier, split-plot experiments are often an economic alternative to fully randomized designs. Split-plot experiments, with their structure of subplot arrays imbedded within whole-plot arrays, have a tendency to become large,
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Construction of an experimental plot seeder of wheat planting and compare it by imported one
Directory of Open Access Journals (Sweden)
I Eskandari
2016-09-01
spinner can be equipped with a 4 or 6 outlet delivery head, depending on row spacing. The planter is fitted with hoe openers. Alternatively, spear-point openers have sometimes been used under conventional tillage systems. Seeding depth control was achieved by an adjustment screw handle. The plot seeder is being moved by a 9.6 kW engine, and has been successfully used in applications. Field experiment established by using 4 plot length (2, 3, 4 and 6 with 4 replication by the constructed plot seeder and imported plot seeder. Crop measurements were planted height, spike m-2, seeds/spike, Thousand kernel weight, Biological and grain yield, harvest index and drill measurements were seeding depth, uniformity of row spacing in action, seed counter performance, power requirement, slippage evenly of rows after planting. Results and Discussion Results showed that there were significant differences between the plant emergences. The emergencies were higher in plots, which planted by the new plot seeder. The differences between seed distribution of openers were insignificant, but the variances of new plot seeder and imported plot seeder were 0.267 and 1.05 respectively. Mean planting depth of plots planted by the Wintersteiger plot seeder was 0.8 cm shallower than the adjusted planting depth while mean planting depth in plots planted by constructing machine had only 0.01 cm variation. Results of variance analysis revealed that effect of treatments on wheat grain yield and yield components was significant. So that, highest grain yield (4216 kgha-1, biological yield (8704 kgha-1, number of spikes per square meter (649spike, obtained from a plot which planted by constructed plot seeder. Increasing yield of treatments which planted by constructed plot seeder might be because of increasing the number of spikes per square meter in those treatments. The mean of spike per square meter in plots of new planter was 691 spikes which were116 spike more than plots planted by imported plot seeder
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Shi, Pu; Arter, Christian; Liu, Xingyu; Keller, Martin; Schulin, Rainer
2017-04-01
Aggregate stability is an important factor in soil resistance against erosion, and, by influencing the extent of sediment transport associated with surface runoff, it is thus also one of the key factors which determine on- and off-site effects of water erosion. As it strongly depends on soil organic matter, many studies have explored how aggregate stability can be improved by organic matter inputs into the soil. However, the focus of these studies has been on the relationship between aggregate stability and soil organic matter dynamics. How the effects of organic matter inputs on aggregate stability translate into soil erodibility under rainfall impacts has received much less attention. In this study, we performed field plot experiments to examine how organic matter inputs affect aggregate breakdown and surface sediment transport under field conditions in artificial rainfall events. Three pairs of plots were prepared by adding a mixture of grass and wheat straw to one of plots in each pair but not to the other, while all plots were treated in the same way otherwise. The rainfall events were applied some weeks later so that the applied organic residues had sufficient time for decomposition and incorporation into the soil. Surface runoff rate and sediment concentration showed substantial differences between the treatments with and without organic matter inputs. The plots with organic inputs had coarser and more stable aggregates and a rougher surface than the control plots without organic inputs, resulting in a higher infiltration rate and lower transport capacity of the surface runoff. Consequently, sediments exported from the amended plots were less concentrated but more enriched in suspended particles (selective sediment transport. In contrast to the amended plots, there was an increase in the coarse particle fraction (> 250 µm) in the runoff from the plots with no organic matter inputs towards the end of the rainfall events due to emerging bed-load transport
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International Nuclear Information System (INIS)
Hnatowich, M.; LaBella, F.S.
1982-01-01
Erythrosine (ERY) (FD and C red no. 3) inhibited specific binding of [ 3 H]ouabain to rat brain homogenates with an IC50 of 23 microM in the dark and 1 microM in ordinary fluorescent light. Competition studies demonstrated the presence of two components, only one of which was affected by light. Lineweaver-Burk analysis indicated that ERY preferentially antagonizes [ 3 H]ouabain binding at a high-affinity site in the light, whereas in the dark the dye inhibits binding in a manner qualitatively similar to inhibition by ouabain. Light enhancement of ERY potency occurred only when dye and tissue were present together in the incubation medium, pointing to participation of transient molecular species. However, neither superoxide dismutase nor catalase altered the effects of ERY in the light or dark, suggesting the absence of oxygen free radicals. When oxygen levels were raised, there was enhancement of inhibition by ERY at a high-affinity receptor accompanied by disappearance of [ 3 H]ouabain binding at one of lower affinity. In contrast to brain, membranes from guinea pig heart showed only one binding site for [ 3 H]ouabain, and antagonism by ERY at this site was markedly enhanced by light. Structural differences between classes of ouabain binding regions probably accounts for the discrimination exhibited by ERY in the presence of light and oxygen. Our findings also caution that metabolic transformation of this common food dye, light decomposition, or photoreaction with foodstuff may yield more toxic derivatives
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Directory of Open Access Journals (Sweden)
Hong Xia Chen
2014-10-01
Full Text Available Solvent extracts of Rhus verniciflua Stokes wood were made using decompressing inner ebullition, and a Box-Behnken design was used to optimize extraction conditions to produce an extract that inhibited tyrosinase activity. The chemical compositions and inhibition rates were determined in extracts made with petroleum ether, ethyl acetate, n-butanol, and an aqueous fractionation. The ethyl acetate fraction had the highest total phenolic content and inhibition rates. The main flavonoids in this fraction were 0.531% fisetin, 7.582% fustin, 0.848% sulfuretin, and 0.272% butein. The effects of the extract on the monophenolase and diphenolase activity of mushroom tyrosinase were studied using the Lineweaver-Burk equation to determine the effect of the extract on inhibition of tyrosinase activity. The results showed that the extract inhibited both the monophenolase and diphenolase activity of the enzyme. The IC50 of the ethyl acetate extract was 308 μg/mL, with the lag period of the enzyme being obviously lengthened; it was estimated to be 2.45 min in the absence of the inhibitor and extended to 9.63 min in the presence of 500 μg/mL of extract. The ethyl acetate extract acted as a mixed type inhibitor. The KI was less than the KIS, which demonstrates that the [ESI] is less stable than [EI], suggesting that the extract could easily combine with free enzyme in the enzyme catalysis system, thus affecting enzyme catalysis on the substrate.
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Transport of cyazofamid and kresoxim methyl in runoff at the plot and catchment scales
Lefrancq, Marie; Joaquín García Verdú, Antonio; Maillard, Elodie; Imfeld, Gwenaël; Payraudeau, Sylvain
2013-04-01
Surface runoff and erosion during the course of rainfall events represent major processes of pesticides transport from agricultural land to aquatic ecosystem. In general, field and catchment studies on pesticide transfer are carried out separately. A study at both scales may enable to improve the understanding of scale effects on processes involved in pesticides transport and to give clues on the source areas within an agricultural catchment. In this study, the transport in runoff of two widely used fungicides, i.e. kresoxim methyl (KM) and cyazofamid (CY) was assessed in a 43 ha vineyard catchment and the relative contribution of the total fungicides export from one representative plot was evaluated. During an entire period of fungicide application, from May to August 2011, the discharge and loads of dissolved and particle-laden KM and CY were monitored at the plot and catchment scales. The results showed larger export coefficient of KM and CY from catchment (0.064 and 0.041‰ for KM and CY respectively) than from the studied plot (0.009 and 0.023 ‰ for KM and CY respectively). It suggests that the plot margins especially the road network contributed as well to the fungicide loads. This result underlines the impact of fungicide drift on non-target areas. Furthermore, a larger rainfall threshold is necessary at the plot scale to trigger runoff and mobilise pesticides than on the road network. At the plot scale, a rapid dissipation of the both fungicides in the top soil was observed. It highlights that the risky period encompasses the first rainfall events triggering runoff after the applications. At both scales, KM and CY were not detected in suspended solids (i.e. > 0.7 µm). However their partitioning in runoff water differed. 64.1 and 91.8% of the KM load was detected in the dissolved phase (i.e. particulate phase (i.e. between 0.22 and 0.7 µm) at the plot and catchment scales respectively. Although KM and CY have similar lab-defined properties, our results
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TAURUS, Post-processor of 3-D Finite Elements Plots
International Nuclear Information System (INIS)
Brown, B.E.; Hallquist, J.O.; Kennedy, T.
2002-01-01
Description of program or function: TAURUS reads the binary plot files generated by the LLNL three-dimensional finite element analysis codes, NIKE3D (NESC 9725), DYNA3D (NESC 9909), TACO3D (NESC 9838), TOPAZ3D (NESC9599) and GEMINI and plots contours, time histories, and deformed shapes. Contours of a large number of quantities may be plotted on meshes consisting of plate, shell, and solid type elements. TAURUS can compute a variety of strain measures, reaction forces along constrained boundaries, and momentum. TAURUS has three phases: initialization, geometry display with contouring, and time history processing
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9 CFR 108.6 - Revision of plot plans, blueprints, and legends.
2010-01-01
... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Revision of plot plans, blueprints... FACILITY REQUIREMENTS FOR LICENSED ESTABLISHMENTS § 108.6 Revision of plot plans, blueprints, and legends... changes affecting the workflow are to be made. The licensee shall: (a) Prepare revised plot plans...
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Generalised recurrence plot analysis for spatial data
International Nuclear Information System (INIS)
Marwan, Norbert; Kurths, Juergen; Saparin, Peter
2007-01-01
Recurrence plot based methods are highly efficient and widely accepted tools for the investigation of time series or one-dimensional data. We present an extension of the recurrence plots and their quantifications in order to study recurrent structures in higher-dimensional spatial data. The capability of this extension is illustrated on prototypical 2D models. Next, the tested and proved approach is applied to assess the bone structure from CT images of human proximal tibia. We find that the spatial structures in trabecular bone become more recurrent during the bone loss in osteoporosis
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Selecting the optimum plot size for a California design-based stream and wetland mapping program.
Lackey, Leila G; Stein, Eric D
2014-04-01
Accurate estimates of the extent and distribution of wetlands and streams are the foundation of wetland monitoring, management, restoration, and regulatory programs. Traditionally, these estimates have relied on comprehensive mapping. However, this approach is prohibitively resource-intensive over large areas, making it both impractical and statistically unreliable. Probabilistic (design-based) approaches to evaluating status and trends provide a more cost-effective alternative because, compared with comprehensive mapping, overall extent is inferred from mapping a statistically representative, randomly selected subset of the target area. In this type of design, the size of sample plots has a significant impact on program costs and on statistical precision and accuracy; however, no consensus exists on the appropriate plot size for remote monitoring of stream and wetland extent. This study utilized simulated sampling to assess the performance of four plot sizes (1, 4, 9, and 16 km(2)) for three geographic regions of California. Simulation results showed smaller plot sizes (1 and 4 km(2)) were most efficient for achieving desired levels of statistical accuracy and precision. However, larger plot sizes were more likely to contain rare and spatially limited wetland subtypes. Balancing these considerations led to selection of 4 km(2) for the California status and trends program.
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D.3.3 PLOT Persuasive Learning Design Framework
DEFF Research Database (Denmark)
Gram-Hansen, Sandra Burri
2012-01-01
In this third and final deliverable of WP3: Persuasive Learning Designs, the theoretical cross field between persuasion and learning and the practical analysis of the technological learning tools and products which are currently related to the PLOT project, namely the GLOMaker and the 3ET tool......, are linked together as persuasive learning designs are defined and exemplified through the four e-PLOT cases. Based on the literary study of D.3.1 as well as the subsequent discussions and reflections regarding the theoretical foundation and practical application of persuasive learning technologies......-PLOT work cases. In conclusion, the report presents a number of suggestions regarding the improvement of the two learning tools, which from a theoretical perspective will enhance the persuasive potential, and which can be taken into consideration in WP4 and 5....
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9 CFR 108.7 - Filing of plot plans, blueprints, and legends.
2010-01-01
... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Filing of plot plans, blueprints, and... REQUIREMENTS FOR LICENSED ESTABLISHMENTS § 108.7 Filing of plot plans, blueprints, and legends. Three copies of all plot plans, blueprints, and legends, including revisions, shall be submitted to Animal and Plant...
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Energy Technology Data Exchange (ETDEWEB)
Harris, T.L.
1988-07-01
The Rival and Midale subintervals (Charles Formation, Upper Mississippian), north-central Burke County, North Dakota, represent two relative sea level fluctuations. Updip (northeast), the Rival subinterval contains fine to medium-bedded and chicken-wire anhydrite with interbedded algal bindstone that was deposited on supratidal flats. Basinward (southwest), the lithology changes to oncolitic, peloidal, intraclastic grainstone/packstone that was deposited in intertidal and subtidal restricted lagoonal environments. Evaporites precipitated in the sediment of the intertidal to shallow subtidal restricted lagoonal environment. Overlying the Rival subinterval is skeletal wackestone and packstone of the lower Midale subinterval. The presence of normal-marine fauna (crinoids, brachiopods, trilobites, rugose and tabulate coral) indicates a significant relative sea level transgression occurred following deposition of the Rival. The middle and upper Midale subinterval consists of intensely burrowed dolowackestone and dolomudstone that contain a less diversified faunal assemblage. Overlying the Midale carbonates is a transitional zone of calcareous shale and dolomite that grades upward into mottled (burrowed.) and finely laminated microgranular dolomite and anhydrite. The upper Midale section represents a relative sea level regression (shoreline progradation). Updip (northeast) reservoirs produce from the Midale carbonates, which are sealed laterally and vertically by calcarous shale and microgranular dolomitic anhydrite of the Midale Evaporite. Downdip (southwest), the Rival produces from porous grainstone, which is sealed laterally by intertidal/supratidal carbonates and evaporites, resulting in a stratigraphic trap. Vuggy and intergranular porosity are the major porosity types in the Rival grainstone, and moldic and intercrystalline porosity are dominant in the Midale dolowackestone.
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SEGY to ASCII Conversion and Plotting Program 2.0
Goldman, Mark R.
2005-01-01
INTRODUCTION SEGY has long been a standard format for storing seismic data and header information. Almost every seismic processing package can read and write seismic data in SEGY format. In the data processing world, however, ASCII format is the 'universal' standard format. Very few general-purpose plotting or computation programs will accept data in SEGY format. The software presented in this report, referred to as SEGY to ASCII (SAC), converts seismic data written in SEGY format (Barry et al., 1975) to an ASCII data file, and then creates a postscript file of the seismic data using a general plotting package (GMT, Wessel and Smith, 1995). The resulting postscript file may be plotted by any standard postscript plotting program. There are two versions of SAC: one version for plotting a SEGY file that contains a single gather, such as a stacked CDP or migrated section, and a second version for plotting multiple gathers from a SEGY file containing more than one gather, such as a collection of shot gathers. Note that if a SEGY file has multiple gathers, then each gather must have the same number of traces per gather, and each trace must have the same sample interval and number of samples per trace. SAC will read several common standards of SEGY data, including SEGY files with sample values written in either IBM or IEEE floating-point format. In addition, utility programs are present to convert non-standard Seismic Unix (.sux) SEGY files and PASSCAL (.rsy) SEGY files to standard SEGY files. SAC allows complete user control over all plotting parameters including label size and font, tick mark intervals, trace scaling, and the inclusion of a title and descriptive text. SAC shell scripts create a postscript image of the seismic data in vector rather than bitmap format, using GMT's pswiggle command. Although this can produce a very large postscript file, the image quality is generally superior to that of a bitmap image, and commercial programs such as Adobe Illustrator
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Runoff and soil erosion of field plots in a subtropical mountainous region of China
Fang, N. F.; Wang, L.; Shi, Z. H.
2017-09-01
Anthropogenic pressure coupled with strong precipitation events and a mountainous landscape have led to serious soil erosion and associated problems in the subtropical climate zone of China. This study analyzes 1576 rainfall-runoff-soil loss events at 36 experimental plots (a total of 148 plot-years of data) under a wide range of conditions in subtropical mountainous areas of China where slope farming is commonly practiced. The plots, which have standardized dimensions, represent five common types of land use and have four different slopes. Event-based analyses show that almost half of the total rainfall caused soil erosion in the study area. The dominant factor controlling the runoff coefficient is the slope gradient rather than the land use type. The maximum soil lossfor crop plots under steep tillage (35°) is 5004 t km-2 for a single event. Among the common local crops, the average soil loss values increase in the following order: buckwheat soil loss increase in the following order: red clover soil loss is caused by a small number of extreme events. The annual average soil loss of the 44 plots ranges from 19 to 4090 t km-2 year-1. The annual soil loss of plots of different land use types decrease in the following order: bare land (1533 t km-2 year-1) > cropland (1179 t km-2 year-1) > terraced cropland (1083 t km-2 year-1) > orchard land (1020 t km-2 year-1) > grassland (762 t km-2 year-1) > terraced orchard land (297 t km-2 year-1) > forest and grassland (281 t km-2 year-1).
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Impact of Animal Waste Application on Runoff Water Quality in Field Experimental Plots
Directory of Open Access Journals (Sweden)
Paul B. Tchounwou
2005-08-01
Full Text Available Animal waste from dairy and poultry operations is an economical and commonly used fertilizer in the state of Louisiana. The application of animal waste to pasture lands not only is a source of fertilizer, but also allows for a convenient method of waste disposal. The disposal of animal wastes on land is a potential nonpoint source of water degradation. Water degradation and human health is a major concern when considering the disposal of large quantities of animal waste. The objective of this research was to determine the effect of animal waste application on biological (fecal coliform, Enterobacter spp. and Escherichia coli and physical/chemical (temperature, pH, nitrate nitrogen, ammonia nitrogen, phosphate, copper, zinc, and sulfate characteristics of runoff water in experimental plots. The effects of the application of animal waste have been evaluated by utilizing experimental plots and simulated rainfall events. Samples of runoff water were collected and analyzed for fecal coliforms. Fecal coliforms isolated from these samples were identified to the species level. Chemical analysis was performed following standard test protocols. An analysis of temperature, ammonia nitrogen, nitrate nitrogen, iron, copper, phosphate, potassium, sulfate, zinc and bacterial levels was performed following standard test protocols as presented in Standard Methods for the Examination of Water and Wastewater [1]. In the experimental plots, less time was required in the tilled broiler litter plots for the measured chemicals to decrease below the initial pre-treatment levels. A decrease of over 50% was noted between the first and second rainfall events for sulfate levels. This decrease was seen after only four simulated rainfall events in tilled broiler litter plots whereas broiler litter plots required eight simulated rainfall events to show this same type of reduction. A reverse trend was seen in the broiler litter plots and the tilled broiler plots for potassium
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Polar plot representation of time-resolved fluorescence.
Eichorst, John Paul; Wen Teng, Kai; Clegg, Robert M
2014-01-01
Measuring changes in a molecule's fluorescence emission is a common technique to study complex biological systems such as cells and tissues. Although the steady-state fluorescence intensity is frequently used, measuring the average amount of time that a molecule spends in the excited state (the fluorescence lifetime) reveals more detailed information about its local environment. The lifetime is measured in the time domain by detecting directly the decay of fluorescence following excitation by short pulse of light. The lifetime can also be measured in the frequency domain by recording the phase and amplitude of oscillation in the emitted fluorescence of the sample in response to repetitively modulated excitation light. In either the time or frequency domain, the analysis of data to extract lifetimes can be computationally intensive. For example, a variety of iterative fitting algorithms already exist to determine lifetimes from samples that contain multiple fluorescing species. However, recently a method of analysis referred to as the polar plot (or phasor plot) is a graphical tool that projects the time-dependent features of the sample's fluorescence in either the time or frequency domain into the Cartesian plane to characterize the sample's lifetime. The coordinate transformations of the polar plot require only the raw data, and hence, there are no uncertainties from extensive corrections or time-consuming fitting in this analysis. In this chapter, the history and mathematical background of the polar plot will be presented along with examples that highlight how it can be used in both cuvette-based and imaging applications.
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An ecoinformatics application for forest dynamics plot data management and sharing
Chau-Chin Lin; Abd Rahman Kassim; Kristin Vanderbilt; Donald Henshaw; Eda C. Melendez-Colom; John H. Porter; Kaoru Niiyama; Tsutomu Yagihashi; Sek Aun Tan; Sheng-Shan Lu; Chi-Wen Hsiao; Li-Wan Chang; Meei-Ru. Jeng
2011-01-01
Several forest dynamics plot research projects in the East-Asia Pacific region of the International Long-Term Ecological Research network actively collect long-term data, and some of these large plots are members of the Center for Tropical Forest Science network. The wealth of forest plot data presents challenges in information management to researchers. In order to...
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Basu, Sankar
2017-12-07
The complementarity plot (CP) is an established validation tool for protein structures, applicable to both globular proteins (folding) as well as protein-protein complexes (binding). It computes the shape and electrostatic complementarities (S m , E m ) for amino acid side-chains buried within the protein interior or interface and plots them in a two-dimensional plot having knowledge-based probabilistic quality estimates for the residues as well as for the whole structure. The current report essentially presents an upgraded version of the plot with the implementation of the advanced multi-dielectric functionality (as in Delphi version 6.2 or higher) in the computation of electrostatic complementarity to make the validation tool physico-chemically more realistic. The two methods (single- and multi-dielectric) agree decently in their resultant E m values, and hence, provisions for both methods have been kept in the software suite. So to speak, the global electrostatic balance within a well-folded protein and/or a well-packed interface seems only marginally perturbed by the choice of different internal dielectric values. However, both from theoretical as well as practical grounds, the more advanced multi-dielectric version of the plot is certainly recommended for potentially producing more reliable results. The report also presents a new methodology and a variant plot, namely CP dock , based on the same principles of complementarity specifically designed to be used in the docking of proteins. The efficacy of the method to discriminate between good and bad docked protein complexes has been tested on a recent state-of-the-art docking benchmark. The results unambiguously indicate that CP dock can indeed be effective in the initial screening phase of a docking scoring pipeline before going into more sophisticated and computationally expensive scoring functions. CP dock has been made available at https://github.com/nemo8130/CPdock . Graphical Abstract An example showing
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Effects of plot size on forest-type algorithm accuracy
James A. Westfall
2009-01-01
The Forest Inventory and Analysis (FIA) program utilizes an algorithm to consistently determine the forest type for forested conditions on sample plots. Forest type is determined from tree size and species information. Thus, the accuracy of results is often dependent on the number of trees present, which is highly correlated with plot area. This research examines the...
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Considerations in Forest Growth Estimation Between Two Measurements of Mapped Forest Inventory Plots
Michael T. Thompson
2006-01-01
Several aspects of the enhanced Forest Inventory and Analysis (FIA) program?s national plot design complicate change estimation. The design incorporates up to three separate plot sizes (microplot, subplot, and macroplot) to sample trees of different sizes. Because multiple plot sizes are involved, change estimators designed for polyareal plot sampling, such as those...
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Fitting diameter distribution models to data from forest inventories with concentric plot design
Energy Technology Data Exchange (ETDEWEB)
Nanos, N.; Sjöstedt de Luna, S.
2017-11-01
Aim: Several national forest inventories use a complex plot design based on multiple concentric subplots where smaller diameter trees are inventoried when lying in the smaller-radius subplots and ignored otherwise. Data from these plots are truncated with threshold (truncation) diameters varying according to the distance from the plot centre. In this paper we designed a maximum likelihood method to fit the Weibull diameter distribution to data from concentric plots. Material and methods: Our method (M1) was based on multiple truncated probability density functions to build the likelihood. In addition, we used an alternative method (M2) presented recently. We used methods M1 and M2 as well as two other reference methods to estimate the Weibull parameters in 40000 simulated plots. The spatial tree pattern of the simulated plots was generated using four models of spatial point patterns. Two error indices were used to assess the relative performance of M1 and M2 in estimating relevant stand-level variables. In addition, we estimated the Quadratic Mean plot Diameter (QMD) using Expansion Factors (EFs). Main results: Methods M1 and M2 produced comparable estimation errors in random and cluster tree spatial patterns. Method M2 produced biased parameter estimates in plots with inhomogeneous Poisson patterns. Estimation of QMD using EFs produced biased results in plots within inhomogeneous intensity Poisson patterns. Research highlights:We designed a new method to fit the Weibull distribution to forest inventory data from concentric plots that achieves high accuracy and precision in parameter estimates regardless of the within-plot spatial tree pattern.
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Cross-plotting of rock properties for fluid discrimination using well ...
African Journals Online (AJOL)
properties and their attributes (combination of rock properties) cross-plots have been attempted using well data from an offshore field of the Niger Delta as a case study. Numerous cross-plotting techniques of rock- properties/attributes derived from ...
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The Effect of Plot Size on Some Pratylenchus Penetrans ...
African Journals Online (AJOL)
Pratylenchus penetrans counts obtained from a rose field, sampled sequentially by decreasing the plot sizes were computed to obtain the respective sample means, variance and k-value of the negative binomial distribution. Plots 21 m x 80 m, 3.6 m x 3.6 m and 0.6 m x 0.6 m were sampled for the nematode. It is reported ...
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[Heart rate variability study based on a novel RdR RR Intervals Scatter Plot].
Lu, Hongwei; Lu, Xiuyun; Wang, Chunfang; Hua, Youyuan; Tian, Jiajia; Liu, Shihai
2014-08-01
On the basis of Poincare scatter plot and first order difference scatter plot, a novel heart rate variability (HRV) analysis method based on scatter plots of RR intervals and first order difference of RR intervals (namely, RdR) was proposed. The abscissa of the RdR scatter plot, the x-axis, is RR intervals and the ordinate, y-axis, is the difference between successive RR intervals. The RdR scatter plot includes the information of RR intervals and the difference between successive RR intervals, which captures more HRV information. By RdR scatter plot analysis of some records of MIT-BIH arrhythmias database, we found that the scatter plot of uncoupled premature ventricular contraction (PVC), coupled ventricular bigeminy and ventricular trigeminy PVC had specific graphic characteristics. The RdR scatter plot method has higher detecting performance than the Poincare scatter plot method, and simpler and more intuitive than the first order difference method.
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Flyby Error Analysis Based on Contour Plots for the Cassini Tour
Stumpf, P. W.; Gist, E. M.; Goodson, T. D.; Hahn, Y.; Wagner, S. V.; Williams, P. N.
2008-01-01
The maneuver cancellation analysis consists of cost contour plots employed by the Cassini maneuver team. The plots are two-dimensional linear representations of a larger six-dimensional solution to a multi-maneuver, multi-encounter mission at Saturn. By using contours plotted with the dot product of vectors B and R and the dot product of vectors B and T components, it is possible to view the effects delta V on for various encounter positions in the B-plane. The plot is used in operations to help determine if the Approach Maneuver (ensuing encounter minus three days) and/or the Cleanup Maneuver (ensuing encounter plus three days) can be cancelled and also is a linear check of an integrated solution.
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Directory of Open Access Journals (Sweden)
Parawee Rattanakit
2014-01-01
Full Text Available A monolith based immobilized acetylcholinesterase (AChE flow-through reactor has been developed for the determination of copper(II using flow injection spectrophotometric system. The bioreactor was prepared inside a microcapillary column by in situ polymerization of butyl methacrylate, ethylene dimethacrylate, and 2,2-dimethoxy-1,2-diphynyletane-1-one in the presence of 1-decanol, followed by vinyl azlactone functionalization and AChE immobilization. The behavior of AChE before and after being immobilized on the monolith was evaluated by kinetic parameters from Lineweaver and Burk equation. The detection was based on measuring inhibition effect on the enzymatic activity of AChE by copper(II using Ellman’s reaction with spectrophotometric detection at 410 nm. The linear range of the calibration graph was obtained over the range of 0.02–3.00 mg L−1. The detection limit, defined as 10% inhibition (I10, was found to be 0.04 mg L−1. The repeatability was 3.35 % (n=5 for 1.00 mg L−1 of copper(II. The proposed method was applied to the determination of copper(II in natural water samples with sampling rate of 4 h−1.
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Experimental Garden Plots for Botany Lessons
Gorodnicheva, V. V.; Vasil'eva, E. I.
1976-01-01
Discussion of the botany lessons used at two schools points out the need for fifth and sixth grade students to be taught the principles of plant life through observations made at an experimental garden plot at the school. (ND)
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Using the Arduino with MakerPlot Software for the Display of Electrical Device Characteristics
Atkin, Keith
2017-01-01
This paper shows how very simple circuitry attached to an Arduino microcontroller with MakerPlot software can be used for the display of electrical characteristic curves of three commonly available devices: an ohmic resistor, an LED, and a tungsten-filament bulb.
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Contemplation on Plot and Personification in Tehran Makhuf
Mohammad Pashaei; Mirjalil Akrami; Mirjalil Akrami
2013-01-01
 Abstract Story is one of the important and influential literary genres that deserves to research on its structure and content .By study and analysis of a story, the reader could comprehend the textual messages and change his attitude toward life and different issues .After defining of the social novel, the author tries to analyze plot and personification in novel of âTehran-e- Makhufâ and respond to the questions on the method employed for plot and personification and analyze it from pl...
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Thiyagalingam, Jeyarajan
2013-06-01
In this paper, we present a novel visualization technique for assisting the observation and analysis of algorithmic complexity. In comparison with conventional line graphs, this new technique is not sensitive to the units of measurement, allowing multivariate data series of different physical qualities (e.g., time, space and energy) to be juxtaposed together conveniently and consistently. It supports multivariate visualization as well as uncertainty visualization. It enables users to focus on algorithm categorization by complexity classes, while reducing visual impact caused by constants and algorithmic components that are insignificant to complexity analysis. It provides an effective means for observing the algorithmic complexity of programs with a mixture of algorithms and black-box software through visualization. Through two case studies, we demonstrate the effectiveness of complexity plots in complexity analysis in research, education and application. © 2013 The Author(s) Computer Graphics Forum © 2013 The Eurographics Association and Blackwell Publishing Ltd.
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Qi, Li; Ding, Xian-bin; Mao, De-qiang; Feng, Lian-gui; Wang, Yu-lin; Jiao, Yan; Zhang, Chun-hua; Lü, Xiao-yan; Li, Hong; Xia, Yi-yin
2013-03-01
To evaluate the effect of comprehensive control and prevention for chronic diseases in demonstration plot of Chongqing. Residents were enrolled through multi-stage stratified random sampling method from 17 districts or counties which had successfully established demonstration plots and 21 districts or counties which had not established demonstration plots (non-demonstration plot for short) yet on May, 2012. Questionnaire was designed to survey awareness of health knowledge, health behaviors and utilization of health supportive tools. The results were analyzed by SPSS 15.0 software. We investigated 15 108 residents, 6156 of which were in demonstration plot and others (8951) were not. The findings revealed the percentage of the people who were aware the national action of health lifestyle in demonstration plot and in non-demonstration plot were 44.4% (2734/6157) and 40.2% (3598/8951), respectively, and the awareness of the hypertension risk of too much sodium were 72.4% (4458/6156) and 67.5% (6042/8951), respectively, and the awareness of the cardinal vascular disease (CVD) risk of obesity and overweight were 77.2% (4753/6157) and 69.6% (6230/8951), respectively. About the residents' health behaviors in demonstration plot and in non-demonstration plot, the utilization rates of salt restriction scoop or pot were 23.5% (1447/6157) and 17.9% (1602/8951), and the utilization rates of oil restriction pot were 16.7% (1028/6157) and 11.8% (1064/8951), respectively. Totally, 33 of the 37 indexes were shown higher in demonstration plot than that in non-demonstration plot (P plot was more effective, and the remarkable improvement of health knowledge and behaviors level had been achieved in demonstration plot.
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Fitting Data to Model: Structural Equation Modeling Diagnosis Using Two Scatter Plots
Yuan, Ke-Hai; Hayashi, Kentaro
2010-01-01
This article introduces two simple scatter plots for model diagnosis in structural equation modeling. One plot contrasts a residual-based M-distance of the structural model with the M-distance for the factor score. It contains information on outliers, good leverage observations, bad leverage observations, and normal cases. The other plot contrasts…
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Zipper plot: visualizing transcriptional activity of genomic regions.
Avila Cobos, Francisco; Anckaert, Jasper; Volders, Pieter-Jan; Everaert, Celine; Rombaut, Dries; Vandesompele, Jo; De Preter, Katleen; Mestdagh, Pieter
2017-05-02
Reconstructing transcript models from RNA-sequencing (RNA-seq) data and establishing these as independent transcriptional units can be a challenging task. Current state-of-the-art tools for long non-coding RNA (lncRNA) annotation are mainly based on evolutionary constraints, which may result in false negatives due to the overall limited conservation of lncRNAs. To tackle this problem we have developed the Zipper plot, a novel visualization and analysis method that enables users to simultaneously interrogate thousands of human putative transcription start sites (TSSs) in relation to various features that are indicative for transcriptional activity. These include publicly available CAGE-sequencing, ChIP-sequencing and DNase-sequencing datasets. Our method only requires three tab-separated fields (chromosome, genomic coordinate of the TSS and strand) as input and generates a report that includes a detailed summary table, a Zipper plot and several statistics derived from this plot. Using the Zipper plot, we found evidence of transcription for a set of well-characterized lncRNAs and observed that fewer mono-exonic lncRNAs have CAGE peaks overlapping with their TSSs compared to multi-exonic lncRNAs. Using publicly available RNA-seq data, we found more than one hundred cases where junction reads connected protein-coding gene exons with a downstream mono-exonic lncRNA, revealing the need for a careful evaluation of lncRNA 5'-boundaries. Our method is implemented using the statistical programming language R and is freely available as a webtool.
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SpectraPLOT, Visualization Package with a User-Friendly Graphical Interface
Sebald, James; Macfarlane, Joseph; Golovkin, Igor
2017-10-01
SPECT3D is a collisional-radiative spectral analysis package designed to compute detailed emission, absorption, or x-ray scattering spectra, filtered images, XRD signals, and other synthetic diagnostics. The spectra and images are computed for virtual detectors by post-processing the results of hydrodynamics simulations in 1D, 2D, and 3D geometries. SPECT3D can account for a variety of instrumental response effects so that direct comparisons between simulations and experimental measurements can be made. SpectraPLOT is a user-friendly graphical interface for viewing a wide variety of results from SPECT3D simulations, and applying various instrumental effects to the simulated images and spectra. We will present SpectraPLOT's ability to display a variety of data, including spectra, images, light curves, streaked spectra, space-resolved spectra, and drilldown plasma property plots, for an argon-doped capsule implosion experiment example. Future SpectraPLOT features and enhancements will also be discussed.
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On the Nature of Earth-Mars Porkchop Plots
Woolley, Ryan C.; Whetsel, Charles W.
2013-01-01
Porkchop plots are a quick and convenient tool to help mission designers plan ballistic trajectories between two bodies. Parameter contours give rise to the familiar 'porkchop' shape. Each synodic period the pattern repeats, but not exactly, primarily due to differences in inclination and non-zero eccentricity. In this paper we examine the morphological features of Earth-to-Mars porkchop plots and the orbital characteristics that create them. These results are compared to idealistic and optimized transfers. Conclusions are drawn about 'good' opportunities versus 'bad' opportunities for different mission applications.
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AirShow 1.0 CFD Software Users' Guide
Mohler, Stanley R., Jr.
2005-01-01
AirShow is visualization post-processing software for Computational Fluid Dynamics (CFD). Upon reading binary PLOT3D grid and solution files into AirShow, the engineer can quickly see how hundreds of complex 3-D structured blocks are arranged and numbered. Additionally, chosen grid planes can be displayed and colored according to various aerodynamic flow quantities such as Mach number and pressure. The user may interactively rotate and translate the graphical objects using the mouse. The software source code was written in cross-platform Java, C++, and OpenGL, and runs on Unix, Linux, and Windows. The graphical user interface (GUI) was written using Java Swing. Java also provides multiple synchronized threads. The Java Native Interface (JNI) provides a bridge between the Java code and the C++ code where the PLOT3D files are read, the OpenGL graphics are rendered, and numerical calculations are performed. AirShow is easy to learn and simple to use. The source code is available for free from the NASA Technology Transfer and Partnership Office.
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Huang, Cheng-Yen; Hsieh, Ming-Ching; Zhou, Qinwei
2017-04-01
Monoclonal antibodies have become the fastest growing protein therapeutics in recent years. The stability and heterogeneity pertaining to its physical and chemical structures remain a big challenge. Tryptophan fluorescence has been proven to be a versatile tool to monitor protein tertiary structure. By modeling the tryptophan fluorescence emission envelope with log-normal distribution curves, the quantitative measure can be exercised for the routine characterization of monoclonal antibody overall tertiary structure. Furthermore, the log-normal deconvolution results can be presented as a two-dimensional plot with tryptophan emission bandwidth vs. emission maximum to enhance the resolution when comparing samples or as a function of applied perturbations. We demonstrate this by studying four different monoclonal antibodies, which show the distinction on emission bandwidth-maximum plot despite their similarity in overall amino acid sequences and tertiary structures. This strategy is also used to demonstrate the tertiary structure comparability between different lots manufactured for one of the monoclonal antibodies (mAb2). In addition, in the unfolding transition studies of mAb2 as a function of guanidine hydrochloride concentration, the evolution of the tertiary structure can be clearly traced in the emission bandwidth-maximum plot.
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[Effects of sampling plot number on tree species distribution prediction under climate change].
Liang, Yu; He, Hong-Shi; Wu, Zhi-Wei; Li, Xiao-Na; Luo, Xu
2013-05-01
Based on the neutral landscapes under different degrees of landscape fragmentation, this paper studied the effects of sampling plot number on the prediction of tree species distribution at landscape scale under climate change. The tree species distribution was predicted by the coupled modeling approach which linked an ecosystem process model with a forest landscape model, and three contingent scenarios and one reference scenario of sampling plot numbers were assumed. The differences between the three scenarios and the reference scenario under different degrees of landscape fragmentation were tested. The results indicated that the effects of sampling plot number on the prediction of tree species distribution depended on the tree species life history attributes. For the generalist species, the prediction of their distribution at landscape scale needed more plots. Except for the extreme specialist, landscape fragmentation degree also affected the effects of sampling plot number on the prediction. With the increase of simulation period, the effects of sampling plot number on the prediction of tree species distribution at landscape scale could be changed. For generalist species, more plots are needed for the long-term simulation.
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Activities: Plotting and Predicting from Pairs.
Shulte, Albert P.; Swift, Jim
1984-01-01
This teacher's guide provides objectives, procedures, and list of materials needed for activities which center around the use of a scatter plot to examine relationships shown by bivariate data. The activities are suitable for grades 7 to 12. Four student worksheets are included. (JN)
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Shiffman, Carl
2017-11-30
To define and elucidate the properties of reduced-variable Nyquist plots. Non-invasive measurements of the electrical impedance of the human thigh. A retrospective analysis of the electrical impedances of 154 normal subjects measured over the past decade shows that 'scaling' of the Nyquist plots for human thigh muscles is a property shared by healthy thigh musculature, irrespective of subject and the length of muscle segment. Here the term scaling signifies the near and sometimes 'perfect' coalescence of the separate X versus R plots into one 'reduced' Nyquist plot by the simple expedient of dividing R and X by X m , the value of X at the reactance maximum. To the extent allowed by noise levels one can say that there is one 'universal' reduced Nyquist plot for the thigh musculature of healthy subjects. There is one feature of the Nyquist curves which is not 'universal', however, namely the frequency f m at which the maximum in X is observed. That is found to vary from 10 to 100 kHz. depending on subject and segment length. Analysis shows, however, that the mean value of 1/f m is an accurately linear function of segment length, though there is a small subject-to-subject random element as well. Also, following the recovery of an otherwise healthy victim of ankle fracture demonstrates the clear superiority of measurements above about 800 kHz, where scaling is not observed, in contrast to measurements below about 400 kHz, where scaling is accurately obeyed. The ubiquity of 'scaling' casts new light on the interpretation of impedance results as they are used in electrical impedance myography and bioelectric impedance analysis.
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Consistent Dalitz plot analysis of Cabibbo-favored D+ → K bar ππ+ decays
Niecknig, Franz; Kubis, Bastian
2018-05-01
We resume the study of the Cabibbo-favored charmed-meson decays D+ → K bar ππ+ in a dispersive framework that satisfies unitarity, analyticity, and crossing symmetry by construction. The formalism explicitly describes the strong final-state interactions between all three decay products and relies on pion-pion and pion-kaon phase shift input. For the first time, we show that the D+ →KSπ0π+ Dalitz plot obtained by the BESIII collaboration as well as the D+ →K-π+π+ Dalitz plot data by CLEO and FOCUS can be described consistently, exploiting the isospin relation between the two coupled decay channels that provides better constraints on the subtraction constants.
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A Guided Inquiry on Hubble Plots and the Big Bang
Forringer, Ted
2014-01-01
In our science for non-science majors course "21st Century Physics," we investigate modern "Hubble plots" (plots of velocity versus distance for deep space objects) in order to discuss the Big Bang, dark matter, and dark energy. There are two potential challenges that our students face when encountering these topics for the…
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Plot - level stem volume estimation and tree species discrimination with CASI remote sensing
Energy Technology Data Exchange (ETDEWEB)
Holmgren, Johan; Wallerman, J.; Olsson, Haakan
1999-10-01
Spectral data from the Compact Airborne Spectrographic Imager (CASI), with four bands (460-495 nm, 550-580 nm, 660-682 nm, 740-762 nm) acquired from a forest test area (Lat. 60 deg 00` N, Long. 17 deg 18` E), the Kaettboele estate near Uppsala, was analysed together with forest data from a number of field plots. Data from two flight lines, one towards and the other perpendicular to the sun was used. Information about stem volume and species composition from plots with 10-m radius, 138 in the first and 120 in the second flight line, was available. There was a positive correlation (R{sup 2} 0.51-0.53) between stem volume and the inverted radiance for all four bands on plot level. The strong correlation between stem volume and a shadow density measure indicates that shadows explain much of the correlation. For the flight line perpendicular to the sun, the correlation was stronger for the side towards the sun compared to the side away from the sun. In the first flight line, plots with a stem volume > 120 m{sup 3}ha{sup -1} were classified according to the tree species composition (pine, spruce, deciduous trees). Groups were formed based on the classification, and the hypothesis that there was no difference in spectral radiance between these groups was tested. It was possible to separate pine dominated plots from spruce dominated plots. It was also possible to separate spruce dominated plots from spruce dominated plots with a minor portion of pine, but not pine dominated plots from pine dominated plots with a minor portion of spruce. The near-infrared band was the best band for discrimination of tree species 16 refs, 2 figs, 8 tabs
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International Nuclear Information System (INIS)
Ma Yugang
2000-01-01
Isospin dependent classical molecular dynamics model is used to investigate the nuclear disassembly of 129 Xe. Zpif-type plot in the field of linguistics is tested for the rank-classified cluster arrangement from this nuclear disassembly. It is found that the average cluster charge (or mass) of rank n in the charge (or mass) list is exactly inverse to its rank, i.e. there exists Zpif's law at the point of the liquid gas phase transition. This novel criterion can be used to diagnose the nuclear liquid gas phase transition experimentally and theoretically
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Dalitz plot studies of D0 → KS0π+π− decays in a factorization approach
Directory of Open Access Journals (Sweden)
Leśniak Leonard
2014-01-01
Full Text Available A quasi two-body QCD factorization is used to study the D0 → KS0π+π− decays. The presently available high-statistics Dalitz plot data of this process measured by the Belle and BABAR Collaborations are analyzed together with the τ− → KS0π−ντ decay data. The total experimental branching fraction is also included in the fits which show a very good overall agreement with the experimental Dalitz plot density distributions. The branching fractions of the dominant channels compare well with those of the isobar Belle or BABAR models. We show that the branching fractions corresponding to the annihilation amplitudes are significant.
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Correlation analysis of respiratory signals by using parallel coordinate plots.
Saatci, Esra
2018-01-01
The understanding of the bonds and the relationships between the respiratory signals, i.e. the airflow, the mouth pressure, the relative temperature and the relative humidity during breathing may provide the improvement on the measurement methods of respiratory mechanics and sensor designs or the exploration of the several possible applications in the analysis of respiratory disorders. Therefore, the main objective of this study was to propose a new combination of methods in order to determine the relationship between respiratory signals as a multidimensional data. In order to reveal the coupling between the processes two very different methods were used: the well-known statistical correlation analysis (i.e. Pearson's correlation and cross-correlation coefficient) and parallel coordinate plots (PCPs). Curve bundling with the number intersections for the correlation analysis, Least Mean Square Time Delay Estimator (LMS-TDE) for the point delay detection and visual metrics for the recognition of the visual structures were proposed and utilized in PCP. The number of intersections was increased when the correlation coefficient changed from high positive to high negative correlation between the respiratory signals, especially if whole breath was processed. LMS-TDE coefficients plotted in PCP indicated well-matched point delay results to the findings in the correlation analysis. Visual inspection of PCB by visual metrics showed range, dispersions, entropy comparisons and linear and sinusoidal-like relationships between the respiratory signals. It is demonstrated that the basic correlation analysis together with the parallel coordinate plots perceptually motivates the visual metrics in the display and thus can be considered as an aid to the user analysis by providing meaningful views of the data. Copyright © 2017 Elsevier B.V. All rights reserved.
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Complex Correlation Measure: a novel descriptor for Poincaré plot
Directory of Open Access Journals (Sweden)
Gubbi Jayavardhana
2009-08-01
Full Text Available Abstract Background Poincaré plot is one of the important techniques used for visually representing the heart rate variability. It is valuable due to its ability to display nonlinear aspects of the data sequence. However, the problem lies in capturing temporal information of the plot quantitatively. The standard descriptors used in quantifying the Poincaré plot (SD1, SD2 measure the gross variability of the time series data. Determination of advanced methods for capturing temporal properties pose a significant challenge. In this paper, we propose a novel descriptor "Complex Correlation Measure (CCM" to quantify the temporal aspect of the Poincaré plot. In contrast to SD1 and SD2, the CCM incorporates point-to-point variation of the signal. Methods First, we have derived expressions for CCM. Then the sensitivity of descriptors has been shown by measuring all descriptors before and after surrogation of the signal. For each case study, lag-1 Poincaré plots were constructed for three groups of subjects (Arrhythmia, Congestive Heart Failure (CHF and those with Normal Sinus Rhythm (NSR, and the new measure CCM was computed along with SD1 and SD2. ANOVA analysis distribution was used to define the level of significance of mean and variance of SD1, SD2 and CCM for different groups of subjects. Results CCM is defined based on the autocorrelation at different lags of the time series, hence giving an in depth measurement of the correlation structure of the Poincaré plot. A surrogate analysis was performed, and the sensitivity of the proposed descriptor was found to be higher as compared to the standard descriptors. Two case studies were conducted for recognizing arrhythmia and congestive heart failure (CHF subjects from those with NSR, using the Physionet database and demonstrated the usefulness of the proposed descriptors in biomedical applications. CCM was found to be a more significant (p = 6.28E-18 parameter than SD1 and SD2 in discriminating
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In-situ polymerization PLOT columns I: divinylbenzene
Shen, T. C.
1992-01-01
A novel method for preparation of porous-layer open-tubular (PLOT) columns is described. The method involves a simple and reproducible, straight-forward in-situ polymerization of monomer directly on the metal tube.
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Hayashi, K; Yamada, T; Sawa, T
2015-03-01
The return or Poincaré plot is a non-linear analytical approach in a two-dimensional plane, where a timed signal is plotted against itself after a time delay. Its scatter pattern reflects the randomness and variability in the signals. Quantification of a Poincaré plot of the electroencephalogram has potential to determine anaesthesia depth. We quantified the degree of dispersion (i.e. standard deviation, SD) along the diagonal line of the electroencephalogram-Poincaré plot (named as SD1/SD2), and compared SD1/SD2 values with spectral edge frequency 95 (SEF95) and bispectral index values. The regression analysis showed a tight linear regression equation with a coefficient of determination (R(2) ) value of 0.904 (p plot tightly correlates with SEF95, reflecting anaesthesia-dependent changes in electroencephalogram oscillation. © 2014 The Association of Anaesthetists of Great Britain and Ireland.
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Maetens, W.; Poesen, J.; Vanmaercke, M.
2012-10-01
The effects of soil and water conservation techniques (SWCTs) on annual runoff (Ra), runoff coefficients (RCa) and annual soil loss (SLa) at the plot scale have been extensively tested on field runoff plots in Europe and the Mediterranean. Nevertheless, a comprehensive overview of these effects and the factors controlling the effectiveness of SWCTs is lacking. Especially the effectiveness of SWCT in reducing Ra is poorly understood. Therefore, an extensive literature review is presented that compiles the results of 101 earlier studies. In each of these studies, Ra and SLa was measured on field runoff plots where various SWCTs were tested. In total, 353 runoff plots (corresponding to 2093 plot-years of data) for 103 plot-measuring stations throughout Europe and the Mediterranean were considered. SWCTs include (1) crop and vegetation management (i.e. cover crops, mulching, grass buffer strips, strip cropping and exclosure), (2) soil management (i.e. no-tillage, reduced tillage, contour tillage, deep tillage, drainage and soil amendment) and (3) mechanical methods (i.e. terraces, contour bunds and geotextiles). Comparison of the frequency distributions of SLa rates on cropland without and with the application of SWCTs shows that the exceedance probability of tolerable SLa rates is ca. 20% lower when SWCT are applied. However, no notable effect of SWCTs on the frequency distribution of RCa is observed. For 224 runoff plots (corresponding to 1567 plot-year data), SWCT effectiveness in reducing Ra and/or SLa could be directly calculated by comparing measured Ra and/or SLa with values measured on a reference plot with conventional management. Crop and vegetation management techniques (i.e. buffer strips, mulching and cover crops) and mechanical techniques (i.e. geotextiles, contour bunds and terraces) are generally more effective than soil management techniques (i.e. no-tillage, reduced tillage and contour tillage). Despite being generally less effective, no
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Thomas, C; Kobold, U; Balan, S; Roeddiger, R; Thomas, L
2011-04-01
Biochemical markers of iron deficiency do not distinguish iron-deficient anemia (IDA) from the anemia of chronic disease (ACD) and the combined state of ACD/IDA. Serum hepcidin-25 might be a marker resolving this problem. We investigated the extent to which serum hepcidin-25 enables the differentiation of the states above in comparison with the ferritin index plot, the so-called Thomas plot [soluble transferrin receptor (sTfR)/log ferritin and the reticulocyte hemoglobin content (CHr)]. Serum hepcidin-25 was determined in 155 anemic patients who were classified as having latent iron deficiency (latent ID), IDA, ACD, or ACD/IDA using the ferritin index plot (Thomas plot). Hepcidin-25 was determined using an isotope-dilution micro-HPLC-tandem mass spectrometry method. The ability to discriminate among these states based on serum hepcidin-25 alone or in combination with the CHr was evaluated in a receiver operating characteristic curve analysis and a comparison with the recently established ferritin index plot. Serum hepcidin-25 correlated with ferritin and the ferritin index. Use of a hepcidin-25 cutoff level of ≤4 nmol/l allowed the differentiation of IDA from ACD and ACD/IDA. Furthermore, the discrimination of ACD/IDA from ACD required combination with CHr in a new plot (hepcidin-25 and the CHr). The hepcidin-25 plot and the ferritin index plot showed a good correspondence in the differentiation of iron states in patients with anemia. Patients with IDA can be differentiated from ACD and ACD/IDA but not ACD from ACD/IDA based on hepcidin-25 alone. The combination of hepcidin-25 with CHr in the hepcidin-25 plot was useful for the differentiation of the states above. © 2010 Blackwell Publishing Ltd.
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A narratological analysis of Mark 12:1-12: The plot of the Gospel of ...
African Journals Online (AJOL)
The purpose of this article is an attempt to read Mark 12: 1-12 in terms of the plot of the Gospel. Firstly a brief survey is given of the development of the term plot from Aristotle to the present, thereafter an own methodological point of departure concerning plot is formulated in order to study the plot of Mark. The conclusions ...
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Coban, T Abdül Kadir; Ciftçi, Mehmet; Küfrevioğlu, O Irfan
2002-05-01
In this study, glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+ oxidoreductase, EC 1.1.1.49; G6PD) was purified from parsley (Petroselinum hortense) leaves, and analysis of the kinetic behavior and some properties of the enzyme were investigated. The purification consisted of three steps: preparation of homogenate, ammonium sulfate fractionation, and DEAE-Sephadex A50 ion exchange chromatography. The enzyme was obtained with a yield of 8.79% and had a specific activity of 2.146 U (mg protein)(-1). The overall purification was about 58-fold. Temperature of +4 degrees C was maintained during the purification process. Enzyme activity was spectrophotometrically measured according to the Beutler method, at 340 nm. In order to control the purification of enzyme, SDS-polyacrylamide gel electrophoresis was carried out in 4% and 10% acrylamide for stacking and running gel, respectively. SDS-polyacrylamide gel electrophoresis showed a single band for enzyme. The molecular weight was found to be 77.6 kDa by Sephadex G-150 gel filtration chromatography. A protein band corresponding to a molecular weight of 79.3 kDa was obtained on SDS-polyacrylamide gel electrophoresis. For the enzymes, the stable pH, optimum pH, and optimum temperature were found to be 6.0, 8.0, and 60 degrees C, respectively. Moreover, KM and Vmax values for NADP+ and G6-P at optimum pH and 25 degrees C were determined by means of Lineweaver-Burk graphs. Additionally, effects of streptomycin sulfate and tetracycline antibiotics were investigated for the enzyme activity of glucose-6-phosphate dehydrogenase in vitro.
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Sreerama, Yadahally N; Neelam, Dennis A; Sashikala, Vadakkoot B; Pratape, Vishwas M
2010-04-14
Milled fractions of chickpea ( Cicer arietinum L.) and horse gram ( Macrotyloma uniflorum L. Verdc.) were evaluated for their nutritional and antinutritional characteristics. Crude protein content of these fractions ranged from 22.6-23.8 g 100(-1) g in cotyledon to 7.3-9.1 g 100(-1) g in seed coat fractions. The fat content of chickpea fractions (1.6-7.8 g 100(-1) g) was higher than that of horse gram fractions (0.6-2.6 g 100(-1) g). Crude fiber content was higher in seed coat fractions of both legumes than embryonic axe and cotyledon fractions. Seed coat fractions had high dietary fiber content (28.2-36.4 g 100(-1) g), made up of mainly insoluble dietary fiber. Most of the phytic acid and oligosaccharides were located in the cotyledon fractions, whereas phenolic compounds in higher concentrations were found in seed coats. Significantly higher concentrations of proteinaceous and phenolic inhibitors of digestive enzymes were found in cotyledon and seed coat fractions, respectively. The kinetic studies, using Michaelis-Menten and Lineweaver-Burk derivations, revealed that seed coat phenolics inhibit alpha-amylase activity by mixed noncompetitive (chickpea) and noncompetitive (horse gram) inhibition mechanisms. In the case of trypsin, chickpea and horse gram seed coat phenolics showed noncompetitive and uncompetitive modes of inhibition, respectively. These results suggest the wide variability in the nutrient and antinutrient composition in different milled fractions of legumes and potential utility of these fractions as ingredients in functional food product development.
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Recurrence plot analysis of DNA sequences
Energy Technology Data Exchange (ETDEWEB)
Wu Zuobing [State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080 (China)]. E-mail: wuzb@lnm.imech.ac.cn
2004-11-15
Recurrence plot technique of DNA sequences is established on metric representation and employed to analyze correlation structure of nucleotide strings. It is found that, in the transference of nucleotide strings, a human DNA fragment has a major correlation distance, but a yeast chromosome's correlation distance has a constant increasing.
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Karmakar, Chandan K; Khandoker, Ahsan H; Voss, Andreas; Palaniswami, Marimuthu
2011-03-03
A novel descriptor (Complex Correlation Measure (CCM)) for measuring the variability in the temporal structure of Poincaré plot has been developed to characterize or distinguish between Poincaré plots with similar shapes. This study was designed to assess the changes in temporal structure of the Poincaré plot using CCM during atropine infusion, 70° head-up tilt and scopolamine administration in healthy human subjects. CCM quantifies the point-to-point variation of the signal rather than gross description of the Poincaré plot. The physiological relevance of CCM was demonstrated by comparing the changes in CCM values with autonomic perturbation during all phases of the experiment. The sensitivities of short term variability (SD1), long term variability (SD2) and variability in temporal structure (CCM) were analyzed by changing the temporal structure by shuffling the sequences of points of the Poincaré plot. Surrogate analysis was used to show CCM as a measure of changes in temporal structure rather than random noise and sensitivity of CCM with changes in parasympathetic activity. CCM was found to be most sensitive to changes in temporal structure of the Poincaré plot as compared to SD1 and SD2. The values of all descriptors decreased with decrease in parasympathetic activity during atropine infusion and 70° head-up tilt phase. In contrast, values of all descriptors increased with increase in parasympathetic activity during scopolamine administration. The concordant reduction and enhancement in CCM values with parasympathetic activity indicates that the temporal variability of Poincaré plot is modulated by the parasympathetic activity which correlates with changes in CCM values. CCM is more sensitive than SD1 and SD2 to changes of parasympathetic activity.
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Ito, Hiroshi; Ikoma, Yoko; Seki, Chie; Kimura, Yasuyuki; Kawaguchi, Hiroshi; Takuwa, Hiroyuki; Ichise, Masanori; Suhara, Tetsuya; Kanno, Iwao
2017-05-01
Objectives In PET studies for neuroreceptors, tracer kinetics are described by the two-tissue compartment model (2-TCM), and binding parameters, including the total distribution volume (V T ), non-displaceable distribution volume (V ND ), and binding potential (BP ND ), can be determined from model parameters estimated by kinetic analysis. The stability of binding parameter estimates depends on the kinetic characteristics of radioligands. To describe these kinetic characteristics, we previously developed a two-phase graphic plot analysis in which V ND and V T can be estimated from the x-intercept of regression lines for early and delayed phases, respectively. In this study, we applied this graphic plot analysis to visual evaluation of the kinetic characteristics of radioligands for neuroreceptors, and investigated a relationship between the shape of these graphic plots and the stability of binding parameters estimated by the kinetic analysis with 2-TCM in simulated brain tissue time-activity curves (TACs) with various binding parameters. Methods 90-min TACs were generated with the arterial input function and assumed kinetic parameters according to 2-TCM. Graphic plot analysis was applied to these simulated TACs, and the curvature of the plot for each TAC was evaluated visually. TACs with several noise levels were also generated with various kinetic parameters, and the bias and variation of binding parameters estimated by kinetic analysis were calculated in each TAC. These bias and variation were compared with the shape of graphic plots. Results The graphic plots showed larger curvature for TACs with higher specific binding and slower dissociation of specific binding. The quartile deviations of V ND and BP ND determined by kinetic analysis were smaller for radioligands with slow dissociation. Conclusions The larger curvature of graphic plots for radioligands with slow dissociation might indicate a stable determination of V ND and BP ND by kinetic analysis. For
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Plot and Personification in “Tehran-e- Makhuf“
Directory of Open Access Journals (Sweden)
M. Pashaei
Full Text Available Story is one of the important and influential literary genres that deserves to research on its structure and content .By study and analysis of a story, the reader could comprehend the textual messages and change his attitude toward life and different issues .After defining of the social novel, the author tries to analyze plot and personification in novel of “Tehran-e- Makhuf” and respond to the questions on the method employed for plot and personification and analyze it from plot and its components such as conflict, suspension and resolution and compare the differences and similarities between old tales and modern novels.The plot of “Tehran-e- Makhuf” is not so complex due to its subject and content and natural order of events dominates on artificial discipline and it lacks open plot. Of main elements of plot, conflict is more outstanding here. This novel is full of emotional and moral conflicts and there is no similarity with historical novels that describe good and bad conflicts. Cause and effect relationship is main element of plot that shadows on the story events .In “Tehrna-e- Makhuf”, the author tries to violate the principles and cause to imbalance in natural events sequences with discomposing rational and experiential relationship and take action toward artificiality and unnatural suspension. In creating suspension by emphasis on incidences in some extremist cases, Kazemi tries to found the story based on the considerable sequence of events and this conduct reduces quality of the story significantly and leads to imbalance in rational and acceptable discipline.Kazemi employs direct or reporting personification mostly. This novel lacks diversity in personage considerably .All characters speak in similar style and there is no particular speech style. The personification of Kazemi in “Tehran-e-Makhuf” is continuous and interdependent in style of old tales and novel in European account. In some cases, he approaches to story
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Contemplation on Plot and Personification in Tehran Makhuf
Directory of Open Access Journals (Sweden)
Mirjalil Akrami
2013-11-01
Full Text Available Abstract Story is one of the important and influential literary genres that deserves to research on its structure and content .By study and analysis of a story, the reader could comprehend the textual messages and change his attitude toward life and different issues .After defining of the social novel, the author tries to analyze plot and personification in novel of “Tehran-e- Makhuf” and respond to the questions on the method employed for plot and personification and analyze it from plot and its components such as conflict, suspension and resolution and compare the differences and similarities between old tales and modern novels. The plot of “Tehran-e- Makhuf” is not so complex due to its subject and content and natural order of events dominates on artificial discipline and it lacks open plot. Of main elements of plot, conflict is more outstanding here. This novel is full of emotional and moral conflicts and there is no similarity with historical novels that describe good and bad conflicts. Cause and effect relationship is main element of plot that shadows on the story events .In “Tehrna-e- Makhuf”, the author tries to violate the principles and cause to imbalance in natural events sequences with discomposing rational and experiential relationship and take action toward artificiality and unnatural suspension. In creating suspension by emphasis on incidences in some extremist cases, Kazemi tries to found the story based on the considerable sequence of events and this conduct reduces quality of the story significantly and leads to imbalance in rational and acceptable discipline. Kazemi employs direct or reporting personification mostly. This novel lacks diversity in personage considerably .All characters speak in similar style and there is no particular speech style. The personification of Kazemi in “Tehran-e-Makhuf” is continuous and interdependent in style of old tales and novel in European account. In some cases, he
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Using 7Be to document soil erosion on the weed plots
International Nuclear Information System (INIS)
Zhang Bo; Zhang Fengbao; Yang Mingyi
2013-01-01
Be tracing technology was applied to document soil erosion on the bare plot and weed plot, and compae soil erosion rate with the calculated rate. Results indicated that vegetation cover had obvious effect on the estimate of soil erosion rate on the weed plot using 7 Be measurement. Therefore, a factor of vegetation had been introduced into the Walling's model of converting 7 Be activity to soil erosion rate for estimating soil erosion rate on the weed slope surface. It was found that the soil erosion rates calculated by modified model were well close to the measured values on the weed plot, which illustrated that the modified model could be well used to estimate the rates of soil loss on the weed slope surface. These findings provide effective means for further study on the relationship between vegetation cover and soil erosion. (authors)
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Directory of Open Access Journals (Sweden)
Ashikhmanova Natalya Alexandrovna
2015-12-01
Full Text Available It is considered the plot device “sacrifice” as a meaningful component of the narrative. It sets a minimum of substantive meaning of this formation (the idea of getting something over the loss of something. The main types of sacrifice: sacrifice and self-sacrifice. Sacrifice means bringing something to sacrifice for the sake of an idea or someone else and implies a greater degree of external attributes. Self-sacrifice brings a loss of the intrinsic value of the character. Sacrifice as the action takes place consciously and, as a rule, does not require compensation in return. Victims sacrifice not only material things but spiritual as well. Their actions are certainly driven by the idea, which is of value. In the case of self-sacrifice victim is itself a victim. Present for sacrifice plays a certain role in the development of some of the stories of fabulous narratives. Search of the present can be a starting point in the development of fantastic narrative plot. As a sacrifice the present can be considered in three forms: in the form of material expression, in the form of immaterial substance, as well as a way to transfer the character into magical power through knowledge empowerment of its formulaic spells.
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Representing Uncertainty on Model Analysis Plots
Smith, Trevor I.
2016-01-01
Model analysis provides a mechanism for representing student learning as measured by standard multiple-choice surveys. The model plot contains information regarding both how likely students in a particular class are to choose the correct answer and how likely they are to choose an answer consistent with a well-documented conceptual model.…
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Shi, Ping; Hu, Sijung; Yu, Hongliu
2018-02-01
The aim of this study was to analyze the recovery of heart rate variability (HRV) after treadmill exercise and to investigate the autonomic nervous system response after exercise. Frequency domain indices, i.e., LF(ms 2 ), HF(ms 2 ), LF(n.u.), HF(n.u.) and LF/HF, and lagged Poincaré plot width (SD1 m ) and length (SD2 m ) were introduced for comparison between the baseline period (Pre-E) before treadmill running and two periods after treadmill running (Post-E1 and Post-E2). The correlations between lagged Poincaré plot indices and frequency domain indices were applied to reveal the long-range correlation between linear and nonlinear indices during the recovery of HRV. The results suggested entirely attenuated autonomic nervous activity to the heart following the treadmill exercise. After the treadmill running, the sympathetic nerves achieved dominance and the parasympathetic activity was suppressed, which lasted for more than 4 min. The correlation coefficients between lagged Poincaré plot indices and spectral power indices could separate not only Pre-E and two sessions after the treadmill running, but also the two sessions in recovery periods, i.e., Post-E1 and Post-E2. Lagged Poincaré plot as an innovative nonlinear method showed a better performance over linear frequency domain analysis and conventional nonlinear Poincaré plot.
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FLP: a field line plotting code for bundle divertor design
International Nuclear Information System (INIS)
Ruchti, C.
1981-01-01
A computer code was developed to aid in the design of bundle divertors. The code can handle discrete toroidal field coils and various divertor coil configurations. All coils must be composed of straight line segments. The code runs on the PDP-10 and displays plots of the configuration, field lines, and field ripple. It automatically chooses the coil currents to connect the separatrix produced by the divertor to the outer edge of the plasma and calculates the required coil cross sections. Several divertor designs are illustrated to show how the code works
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Insights into gait disorders: walking variability using phase plot analysis, Parkinson's disease.
Esser, Patrick; Dawes, Helen; Collett, Johnny; Howells, Ken
2013-09-01
Gait variability may have greater utility than spatio-temporal parameters and can, be an indication for risk of falling in people with Parkinson's disease (PD). Current methods rely on prolonged data collection in order to obtain large datasets which may be demanding to obtain. We set out to explore a phase plot variability analysis to differentiate typically developed adults (TDAs) from PD obtained from two 10 m walks. Fourteen people with PD and good mobility (Rivermead Mobility Index≥8) and ten aged matched TDA were recruited and walked over 10-m at self-selected walking speed. An inertial measurement unit was placed over the projected centre of mass (CoM) sampling at 100 Hz. Vertical CoM excursion was derived to determine modelled spatiotemporal data after which the phase plot analysis was applied producing a cloud of datapoints. SDA described the spread and SDB the width of the cloud with β the angular vector of the data points. The ratio (∀) was defined as SDA: SDB. Cadence (p=.342) and stride length (p=.615) did not show a significance between TDA and PD. A difference was found for walking speed (p=.041). Furthermore a significant difference was found for β (p=.010), SDA (p=.004) other than SDB (p=.385) or ratio ∀ (p=.830). Two sequential 10-m walks showed no difference in PD for cadence (p=.193), stride length (p=.683), walking speed (p=.684) and β (p=.194), SDA (p=.051), SDB (p=.145) or ∀ (p=.226). The proposed phase plot analysis, performed on CoM motion could be used to reliably differentiate PD from TDA over a 10-m walk. Copyright © 2013 Elsevier B.V. All rights reserved.
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Establishing a long-term permanent plot in remnant forest of Cibodas Botanic Garden, West Java
Directory of Open Access Journals (Sweden)
ZAENAL MUTAQIEN
2011-10-01
Full Text Available Mutaqien Z, Zuhri M (2011 Establishing a long-term permanent plot in remnant forest of Cibodas Botanic Garden, West Java. Biodiversitas 12: 218-224. Cibodas Botanic Garden (CBG has unique characters; almost 10% of which is forested area adjacent to the natural forest of Mt. Gede Pangrango National Park. The area is a transition between natural forest and artificial habitat which mostly consists of exotic plant species. The permanent plot in CBG was established in 2007-2009. Two hundred and eighty four units of 10x10 square meters sub-plot were established in four locations, i.e. Wornojiwo, Kompos, Jalan Akar, and Lumut forest. Vegetation analyses were conducted for trees, saplings, shrubs, and herb species. The inventory found 137 species plants consisting of 74 tree species dominated by Villebrunea rubescens (Bl. Bl. and Ostodes paniculata Bl., 30 shrub species dominated by Strobilanthes hamiltoniana (Steud., 24 herb species dominated by Cyrtandra picta Bl., 6 fern species mainly consisted of Diplazium pallidum Moore, and 3 climber species dominated by Calamus reinwardtii Mart. In comparison with the natural forest of Mt. Gede Pangrango National Park, the CBG permanent plot showed a good representative of the vegetation of lower montane forest. A regular monitoring during the successive years is needed to maintain diversity, monitor forest dynamics and anticipate the spread of invasive plant from CBG.
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Energy Technology Data Exchange (ETDEWEB)
Thompson, William L. [Bonneville Power Administration, Portland, OR (US). Environment, Fish and Wildlife
2001-07-01
Monitoring population numbers is important for assessing trends and meeting various legislative mandates. However, sampling across time introduces a temporal aspect to survey design in addition to the spatial one. For instance, a sample that is initially representative may lose this attribute if there is a shift in numbers and/or spatial distribution in the underlying population that is not reflected in later sampled plots. Plot selection methods that account for this temporal variability will produce the best trend estimates. Consequently, I used simulation to compare bias and relative precision of estimates of population change among stratified and unstratified sampling designs based on permanent, temporary, and partial replacement plots under varying levels of spatial clustering, density, and temporal shifting of populations. Permanent plots produced more precise estimates of change than temporary plots across all factors. Further, permanent plots performed better than partial replacement plots except for high density (5 and 10 individuals per plot) and 25% - 50% shifts in the population. Stratified designs always produced less precise estimates of population change for all three plot selection methods, and often produced biased change estimates and greatly inflated variance estimates under sampling with partial replacement. Hence, stratification that remains fixed across time should be avoided when monitoring populations that are likely to exhibit large changes in numbers and/or spatial distribution during the study period. Key words: bias; change estimation; monitoring; permanent plots; relative precision; sampling with partial replacement; temporary plots.
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Directory of Open Access Journals (Sweden)
Luiz Fabrício Gardini Brandão
2017-10-01
Full Text Available Lichens are widely distributed around the world. Their phenolic compounds, consisting mainly of depsides and depsidones, have been extensively studied for important biological activities. More recently, these compounds have been evaluated for their inhibitory activity against enzymes such as tyrosinase, a key agent in melanin biosynthesis. In the present investigation, the depsidone fumarprotocetraric acid isolated from the lichen Cladonia verticillaris (Raddi Fr. was evaluated for its inhibitory activity against this critical enzyme. Kinetic study showed that depsidone at 0.6 mM inhibited tyrosinase activity by 39.8%. Lineweaver–Burk plots revealed that fumarprotocetraric acid can act as an uncompetitive or mixed-type inhibitor, depending on concentration. DOI: http://dx.doi.org/10.17807/orbital.v9i4.999
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Contemplation on Plot and Personification in Tehran Makhuf
Directory of Open Access Journals (Sweden)
Mohammad Pashaei
2013-10-01
Full Text Available  Abstract Story is one of the important and influential literary genres that deserves to research on its structure and content .By study and analysis of a story, the reader could comprehend the textual messages and change his attitude toward life and different issues .After defining of the social novel, the author tries to analyze plot and personification in novel of âTehran-e- Makhufâ and respond to the questions on the method employed for plot and personification and analyze it from plot and its components such as conflict, suspension and resolution and compare the differences and similarities between old tales and modern novels.  The plot of âTehran-e- Makhufâ is not so complex due to its subject and content and natural order of events dominates on artificial discipline and it lacks open plot. Of main elements of plot, conflict is more outstanding here. This novel is full of emotional and moral conflicts and there is no similarity with historical novels that describe good and bad conflicts. Cause and effect relationship is main element of plot that shadows on the story events .In âTehrna-e- Makhufâ, the author tries to violate the principles and cause to imbalance in natural events sequences with discomposing rational and experiential relationship and take action toward artificiality and unnatural suspension. In creating suspension by emphasis on incidences in some extremist cases, Kazemi tries to found the story based on the considerable sequence of events and this conduct reduces quality of the story significantly and leads to imbalance in rational and acceptable discipline.  Kazemi employs direct or reporting personification mostly. This novel lacks diversity in personage considerably .All characters speak in similar style and there is no particular speech style. The personification of Kazemi in âTehran-e-Makhufâ is continuous and interdependent in style of old tales and novel in European
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Luo, Quanzhou; Yue, Guihua; Valaskovic, Gary A; Gu, Ye; Wu, Shiaw-Lin; Karger, Barry L.
2008-01-01
Following on our recent work, on-line one dimensional (1D) and two dimensional (2D) PLOT/LC-ESI-MS platforms using 3.2 m × 10 μm i.d. poly(styrenedivinylbenzene) (PS-DVB) porous layer open tubular (PLOT) columns have been developed to provide robust, high performance and ultrasensitive proteomic analysis. Using a PicoClear tee, the dead volume connection between a 50 μm i.d. PS-DVB monolithic microSPE column and the PLOT column was minimized. The microSPE/PLOT column assembly provided a separation performance similar to that obtained with direct injection onto the PLOT column at a mobile phase flow rate of 20 nL/min. The trace analysis potential of the platform was evaluated using an in-gel tryptic digest sample of a gel fraction (15 to 40 kDa) of a cervical cancer (SiHa) cell line. As an example of the sensitivity of the system, ∼2.5 ng of protein in 2 μL solution, an amount corresponding to 20 SiHa cells, was subjected to on-line microSPE-PLOT/LC-ESIMS/MS analysis using a linear ion trap MS. 237 peptides associated with 163 unique proteins were identified from a single analysis when using stringent criteria associated with a false positive rate less than 1% . The number of identified peptides and proteins increased to 638 and 343, respectively, as the injection amount was raised to ∼45 ng of protein, an amount corresponding to 350 SiHa cells. In comparison, only 338 peptides and 231 unique proteins were identified (false positive rate again less than 1%) from 750 ng of protein from the identical gel fraction, an amount corresponding to 6000 SiHa cells, using a typical 15 cm × 75 μm i.d. packed capillary column. The greater sensitivity, higher recovery, and higher resolving power of the PLOT column resulted in the increased number of identifications from only ∼5% of the injected sample amount. The resolving power of the microSPE/PLOT assembly was further extended by 2D chromatography via combination of the high-efficiency reversed phase PLOT column
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Exploration of the forbidden regions of the Ramachandran plot (ϕ-ψ) with QTAIM.
Momen, Roya; Azizi, Alireza; Wang, Lingling; Ping, Yang; Xu, Tianlv; Kirk, Steven R; Li, Wenxuan; Manzhos, Sergei; Jenkins, Samantha
2017-10-04
A new QTAIM interpretation of the Ramachandran plot is formulated from the most and least facile eigenvectors of the second-derivative matrix of the electron density with a set of 29 magainin-2 peptide conformers. The presence of QTAIM eigenvectors associated with the most and least preferred directions of electronic charge density explained the role of hydrogen bonding, HH contacts and the glycine amino acid monomer in peptide folding. The highest degree of occupation of the QTAIM interpreted Ramachandran plot was found for the glycine amino acid monomer compared with the remaining backbone peptide bonds. The mobility of the QTAIM eigenvectors of the glycine amino acid monomer was higher than for the other amino acids and was comparable to that of the hydrogen bonding, explaining the flexibility of the magainin-2 backbone. We experimented with a variety of hybrid QTAIM-Ramachandran plots to highlight and explain why the glycine amino acid monomer largely occupies the 'forbidden' region on the Ramachandran plot. In addition, the new hybrid QTAIM-Ramachandran plots contained recognizable regions that can be associated with concepts familiar from the conventional Ramachandran plot whilst retaining the character of the QTAIM most and least preferred regions.
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Babamoradi, Hamid; van den Berg, Frans; Rinnan, Åsmund
2016-02-18
In Multivariate Statistical Process Control, when a fault is expected or detected in the process, contribution plots are essential for operators and optimization engineers in identifying those process variables that were affected by or might be the cause of the fault. The traditional way of interpreting a contribution plot is to examine the largest contributing process variables as the most probable faulty ones. This might result in false readings purely due to the differences in natural variation, measurement uncertainties, etc. It is more reasonable to compare variable contributions for new process runs with historical results achieved under Normal Operating Conditions, where confidence limits for contribution plots estimated from training data are used to judge new production runs. Asymptotic methods cannot provide confidence limits for contribution plots, leaving re-sampling methods as the only option. We suggest bootstrap re-sampling to build confidence limits for all contribution plots in online PCA-based MSPC. The new strategy to estimate CLs is compared to the previously reported CLs for contribution plots. An industrial batch process dataset was used to illustrate the concepts. Copyright © 2016 Elsevier B.V. All rights reserved.
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The value of building plots in Italy - pdf
Directory of Open Access Journals (Sweden)
Paolo Rosato
2014-12-01
Full Text Available This paper suggests some analysis on dynamics of building plots value in Italy. This is an important subject, since the study of changes in plots value in space and time enables the evaluation of urban rent, which is essential for a fair and effective planning. The analysis confirm past hypothesis which are well known to real estate market operators. The first resulting fact is that the changes in building plots value are concurrent to those in real estate value and the increase occurring in the last years is in relative terms higher than the past one. This seems to confirm that, during real estate market expansion, most of the increase of buildings’ value is attributable to the land rent. The second observation is about value dynamics over time: it has been observed that, during an expansion in demand, the value significantly increases in central areas, much more than in peripheral ones; this means that absolute rent arising from shortage in urban areas is higher than the differential rent created through urban expansion in peripheral areas. Finally, it was highlighted that the factors affecting the value in building areas play different roles, depending on their location relative to the urban centre, identifying differently structured market segments.
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SPECT-series plot-programs for spectral data for the USC-3 computer system
International Nuclear Information System (INIS)
Kawarasaki, Yuuki
1976-01-01
The programs for spectral data plotting, SPECT- series, have been prepared for use in the USC-3 computer system. They are based on the Basic Plot-Subroutine Unit reported previously and used for plot-processing both γ-ray and TOF-spectra. Two programs can process continuously as many data as required, reading from a magnetic-tape file, being of the same format as that in the Magnetic-Tape Base System. Specification of the plot-processing is as follows: the programs can plot (1) any number of data as required on a sheet, (2) in either linear or logarithmic Y-axis scaling, (3) in the arbitrary channel region, and (4) with variable scale-factor. How to program is described in detail. Usage of the representative programs with example outputs is explained. Problems in the improvement of processing speed and utilization efficiency are also discussed. (auth.)
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Directory of Open Access Journals (Sweden)
Shaun R. Levick
2016-05-01
Full Text Available Abstract Background Monitoring and managing carbon stocks in forested ecosystems requires accurate and repeatable quantification of the spatial distribution of wood volume at landscape to regional scales. Grid-based forest inventory networks have provided valuable records of forest structure and dynamics at individual plot scales, but in isolation they may not represent the carbon dynamics of heterogeneous landscapes encompassing diverse land-management strategies and site conditions. Airborne LiDAR has greatly enhanced forest structural characterisation and, in conjunction with field-based inventories, it provides avenues for monitoring carbon over broader spatial scales. Here we aim to enhance the integration of airborne LiDAR surveying with field-based inventories by exploring the effect of inventory plot size and number on the relationship between field-estimated and LiDAR-predicted wood volume in deciduous broad-leafed forest in central Germany. Results Estimation of wood volume from airborne LiDAR was most robust (R2 = 0.92, RMSE = 50.57 m3 ha−1 ~14.13 Mg C ha−1 when trained and tested with 1 ha experimental plot data (n = 50. Predictions based on a more extensive (n = 1100 plot network with considerably smaller (0.05 ha plots were inferior (R2 = 0.68, RMSE = 101.01 ~28.09 Mg C ha−1. Differences between the 1 and 0.05 ha volume models from LiDAR were negligible however at the scale of individual land-management units. Sample size permutation tests showed that increasing the number of inventory plots above 350 for the 0.05 ha plots returned no improvement in R2 and RMSE variability of the LiDAR-predicted wood volume model. Conclusions Our results from this study confirm the utility of LiDAR for estimating wood volume in deciduous broad-leafed forest, but highlight the challenges associated with field plot size and number in establishing robust relationships between airborne LiDAR and field derived wood volume. We
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Levick, Shaun R; Hessenmöller, Dominik; Schulze, E-Detlef
2016-12-01
Monitoring and managing carbon stocks in forested ecosystems requires accurate and repeatable quantification of the spatial distribution of wood volume at landscape to regional scales. Grid-based forest inventory networks have provided valuable records of forest structure and dynamics at individual plot scales, but in isolation they may not represent the carbon dynamics of heterogeneous landscapes encompassing diverse land-management strategies and site conditions. Airborne LiDAR has greatly enhanced forest structural characterisation and, in conjunction with field-based inventories, it provides avenues for monitoring carbon over broader spatial scales. Here we aim to enhance the integration of airborne LiDAR surveying with field-based inventories by exploring the effect of inventory plot size and number on the relationship between field-estimated and LiDAR-predicted wood volume in deciduous broad-leafed forest in central Germany. Estimation of wood volume from airborne LiDAR was most robust (R 2 = 0.92, RMSE = 50.57 m 3 ha -1 ~14.13 Mg C ha -1 ) when trained and tested with 1 ha experimental plot data (n = 50). Predictions based on a more extensive (n = 1100) plot network with considerably smaller (0.05 ha) plots were inferior (R 2 = 0.68, RMSE = 101.01 ~28.09 Mg C ha -1 ). Differences between the 1 and 0.05 ha volume models from LiDAR were negligible however at the scale of individual land-management units. Sample size permutation tests showed that increasing the number of inventory plots above 350 for the 0.05 ha plots returned no improvement in R 2 and RMSE variability of the LiDAR-predicted wood volume model. Our results from this study confirm the utility of LiDAR for estimating wood volume in deciduous broad-leafed forest, but highlight the challenges associated with field plot size and number in establishing robust relationships between airborne LiDAR and field derived wood volume. We are moving into a forest management era where
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Combining FIA plot data with topographic variables: Are precise locations needed?
Stephen P. Prisley; Huei-Jin Wang; Philip J Radtke; John Coulston
2009-01-01
Plot data from the USFS FIA program could be combined with terrain variables to attempt to explain how terrain characteristics influence forest growth, species composition, productivity, fire behavior, wildlife habitat, and other phenomena. While some types of analyses using FIA data have been shown to be insensitive to precision of plot locations, it has been...
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shinyCircos: an R/Shiny application for interactive creation of Circos plot.
Yu, Yiming; Ouyang, Yidan; Yao, Wen
2018-04-01
Creation of Circos plot is one of the most efficient approaches to visualize genomic data. However, the installation and use of existing tools to make Circos plot are challenging for users lacking of coding experiences. To address this issue, we developed an R/Shiny application shinyCircos, a graphical user interface for interactive creation of Circos plot. shinyCircos can be easily installed either on computers for personal use or on local or public servers to provide online use to the community. Furthermore, various types of Circos plots could be easily generated and decorated with simple mouse-click. shinyCircos and its manual are freely available at https://github.com/venyao/shinyCircos. shinyCircos is deployed at https://yimingyu.shinyapps.io/shinycircos/ and http://shinycircos.ncpgr.cn/ for online use. diana1983941@mail.hzau.edu.cn or yaowen@henau.edu.cn.
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Directory of Open Access Journals (Sweden)
Nadir C. Pallqui
2014-12-01
Full Text Available In this study we evaluated the floristic composition and changes in stored biomass and dynamics over time in 9 permanent plots monitored by RAINFOR (Amazon Forest Inventory Network and located in the lowland Amazon rainforest of the Tambopata National Reserve. Data were acquired in the field using the standardized methodology of RAINFOR. The biomass was estimated using the equation for tropical moist forests of Chave et al. (2005. Biomass dynamics were analyzed, in three separated periods from 2003 to 2011. 64 families, 219 genera and 531 species were recorded. The tree floristic composition is very similar in all plots except for one swamp plot, although but it is also evident that two slightly different forest communities exist in the rest of landscape, apparently related to the age of the ancient river terraces in the area. Mortality and recruitment of individuals averaged 2.12 ± 0.52% and 1.92 ± 0.49%, respectively. The turnover rate is 2.02% per year. Aboveground biomass stored in these forests averages 296.2 ± 33.9 t ha-1. The biomass dynamics show a total net gain of 1.96, 1.69 and –1.23 t ha-1 for period respectively. Prior to the drought of 2010 a change in biomass was found 1.88 t ha-1 yr-1 and post drought was -0.18 t ha-1 yr-1 on average, though the difference is not significant. Demographic analysis suggests a dynamic equilibrium in the plots. The negative balance of biomass observed for the period 2008 – 2011 may be due to the drought of 2010, in which half of the monitored plots experienced negative net biomass change due to mortality of individuals selectively affecting the floristic composition.
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Effects of pocket gopher burrowing on cesium-133 distribution on engineered test plots
International Nuclear Information System (INIS)
Gonzales, G.J.; Saladen, M.T.; Hakonson, T.E.
1995-01-01
Very low levels of radionuclides exist on soil surfaces. Biological factors including vegetation and animal burrowing can influence the fate of these surface contaminants. Animal burrowing introduces variability in radionuclide migration that confounds estimation of nuclide migration pathways, risk assessment, and assessment of waste burial performance. A field study on the surface and subsurface erosional transport of surface-applied 133 Cs as affected by pocket gopher (Thomomys bottae) burrowing was conducted on simulated waste landfill caps at the Los Alamos National Laboratory in north central New Mexico. Surface loss of Cs, adhered to five soil particle size ranges, was measured several times over an 18-mo period while simulated rainfalls were in progress. Gophers reduced Cs surface loss by significant amounts, 43%. Cesium surface loss on plots with only gophers was 0.8 kg totalled for the study period. This compared with 1.4 kg for control plots, 0.5 kg for vegetated plots, and 0.2 kg for plots with both gophers and vegetation. The change in Cs surface loss over time was significant (P -1 ). Vegetation-bearing plots bad significant more total subsurface Cs (μ = 1.7 g kg -1 ) than plots without vegetation (μ = 0.8 g kg -1 ). An average of 97% of the subsurface Cs in plots with vegetation was located in the upper 15 cm of soil (SDR1 + SDR2) compared with 67% for plots without vegetation. Vegetation moderated the influence of gopher activity on the transport of Cs to soil subsurface, and stabilized subsurface Cs by concentrating it in the rhizosphere. Gopher activity may have caused Cs transport to depths below that sampled, 30 cm. The results provide distribution coefficients for models of contaminant migration where animal burrowing occurs. 35 refs., 2 figs., 3 tabs
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Sun, Phillip Zhe; Wang, Yu; Dai, ZhuoZhi; Xiao, Gang; Wu, Renhua
2014-01-01
Chemical exchange saturation transfer (CEST) MRI is sensitive to dilute proteins and peptides as well as microenvironmental properties. However, the complexity of the CEST MRI effect, which varies with the labile proton content, exchange rate and experimental conditions, underscores the need for developing quantitative CEST (qCEST) analysis. Towards this goal, it has been shown that omega plot is capable of quantifying paramagnetic CEST MRI. However, the use of the omega plot is somewhat limited for diamagnetic CEST (DIACEST) MRI because it is more susceptible to direct radio frequency (RF) saturation (spillover) owing to the relatively small chemical shift. Recently, it has been found that, for dilute DIACEST agents that undergo slow to intermediate chemical exchange, the spillover effect varies little with the labile proton ratio and exchange rate. Therefore, we postulated that the omega plot analysis can be improved if RF spillover effect could be estimated and taken into account. Specifically, simulation showed that both labile proton ratio and exchange rate derived using the spillover effect-corrected omega plot were in good agreement with simulated values. In addition, the modified omega plot was confirmed experimentally, and we showed that the derived labile proton ratio increased linearly with creatine concentration (p plot for quantitative analysis of DIACEST MRI. Copyright © 2014 John Wiley & Sons, Ltd.
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Directory of Open Access Journals (Sweden)
Claudia M.C.S. Listopad
2011-06-01
Full Text Available This study used an affordable ground-based portable LiDAR system to provide an understanding of the structural differences between old-growth and secondary-growth Southeastern pine. It provided insight into the strengths and weaknesses in the structural determination of portable systems in contrast to airborne LiDAR systems. Portable LiDAR height profiles and derived metrics and indices (e.g., canopy cover, canopy height were compared among plots with different fire frequency and fire season treatments within secondary forest and old growth plots. The treatments consisted of transitional season fire with four different return intervals: 1-yr, 2-yr, 3-yr fire return intervals, and fire suppressed plots. The remaining secondary plots were treated using a 2-yr late dormant season fire cycle. The old growth plots were treated using a 2-yr growing season fire cycle. Airborne and portable LiDAR derived canopy cover were consistent throughout the plots, with significantly higher canopy cover values found in 3-yr and fire suppressed plots. Portable LiDAR height profile and metrics presented a higher sensitivity in capturing subcanopy elements than the airborne system, particularly in dense canopy plots. The 3-dimensional structures of the secondary plots with varying fire return intervals were dramatically different to old-growth plots, where a symmetrical distribution with clear recruitment was visible. Portable LiDAR, even though limited to finer spatial scales and specific biases, is a low-cost investment with clear value for the management of forest canopy structure.
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An image processing approach to computing distances between RNA secondary structures dot plots
Directory of Open Access Journals (Sweden)
Sapiro Guillermo
2009-02-01
Full Text Available Abstract Background Computing the distance between two RNA secondary structures can contribute in understanding the functional relationship between them. When used repeatedly, such a procedure may lead to finding a query RNA structure of interest in a database of structures. Several methods are available for computing distances between RNAs represented as strings or graphs, but none utilize the RNA representation with dot plots. Since dot plots are essentially digital images, there is a clear motivation to devise an algorithm for computing the distance between dot plots based on image processing methods. Results We have developed a new metric dubbed 'DoPloCompare', which compares two RNA structures. The method is based on comparing dot plot diagrams that represent the secondary structures. When analyzing two diagrams and motivated by image processing, the distance is based on a combination of histogram correlations and a geometrical distance measure. We introduce, describe, and illustrate the procedure by two applications that utilize this metric on RNA sequences. The first application is the RNA design problem, where the goal is to find the nucleotide sequence for a given secondary structure. Examples where our proposed distance measure outperforms others are given. The second application locates peculiar point mutations that induce significant structural alternations relative to the wild type predicted secondary structure. The approach reported in the past to solve this problem was tested on several RNA sequences with known secondary structures to affirm their prediction, as well as on a data set of ribosomal pieces. These pieces were computationally cut from a ribosome for which an experimentally derived secondary structure is available, and on each piece the prediction conveys similarity to the experimental result. Our newly proposed distance measure shows benefit in this problem as well when compared to standard methods used for assessing
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Arif, Muhammad
2012-06-01
In pattern classification problems, feature extraction is an important step. Quality of features in discriminating different classes plays an important role in pattern classification problems. In real life, pattern classification may require high dimensional feature space and it is impossible to visualize the feature space if the dimension of feature space is greater than four. In this paper, we have proposed a Similarity-Dissimilarity plot which can project high dimensional space to a two dimensional space while retaining important characteristics required to assess the discrimination quality of the features. Similarity-dissimilarity plot can reveal information about the amount of overlap of features of different classes. Separable data points of different classes will also be visible on the plot which can be classified correctly using appropriate classifier. Hence, approximate classification accuracy can be predicted. Moreover, it is possible to know about whom class the misclassified data points will be confused by the classifier. Outlier data points can also be located on the similarity-dissimilarity plot. Various examples of synthetic data are used to highlight important characteristics of the proposed plot. Some real life examples from biomedical data are also used for the analysis. The proposed plot is independent of number of dimensions of the feature space.
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Mauya, Ernest William; Hansen, Endre Hofstad; Gobakken, Terje; Bollandsås, Ole Martin; Malimbwi, Rogers Ernest; Næsset, Erik
2015-12-01
Airborne laser scanning (ALS) has recently emerged as a promising tool to acquire auxiliary information for improving aboveground biomass (AGB) estimation in sample-based forest inventories. Under design-based and model-assisted inferential frameworks, the estimation relies on a model that relates the auxiliary ALS metrics to AGB estimated on ground plots. The size of the field plots has been identified as one source of model uncertainty because of the so-called boundary effects which increases with decreasing plot size. Recent research in tropical forests has aimed to quantify the boundary effects on model prediction accuracy, but evidence of the consequences for the final AGB estimates is lacking. In this study we analyzed the effect of field plot size on model prediction accuracy and its implication when used in a model-assisted inferential framework. The results showed that the prediction accuracy of the model improved as the plot size increased. The adjusted R 2 increased from 0.35 to 0.74 while the relative root mean square error decreased from 63.6 to 29.2%. Indicators of boundary effects were identified and confirmed to have significant effects on the model residuals. Variance estimates of model-assisted mean AGB relative to corresponding variance estimates of pure field-based AGB, decreased with increasing plot size in the range from 200 to 3000 m 2 . The variance ratio of field-based estimates relative to model-assisted variance ranged from 1.7 to 7.7. This study showed that the relative improvement in precision of AGB estimation when increasing field-plot size, was greater for an ALS-assisted inventory compared to that of a pure field-based inventory.
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Sampling Error in Relation to Cyst Nematode Population Density Estimation in Small Field Plots.
Župunski, Vesna; Jevtić, Radivoje; Jokić, Vesna Spasić; Župunski, Ljubica; Lalošević, Mirjana; Ćirić, Mihajlo; Ćurčić, Živko
2017-06-01
Cyst nematodes are serious plant-parasitic pests which could cause severe yield losses and extensive damage. Since there is still very little information about error of population density estimation in small field plots, this study contributes to the broad issue of population density assessment. It was shown that there was no significant difference between cyst counts of five or seven bulk samples taken per each 1-m 2 plot, if average cyst count per examined plot exceeds 75 cysts per 100 g of soil. Goodness of fit of data to probability distribution tested with χ 2 test confirmed a negative binomial distribution of cyst counts for 21 out of 23 plots. The recommended measure of sampling precision of 17% expressed through coefficient of variation ( cv ) was achieved if the plots of 1 m 2 contaminated with more than 90 cysts per 100 g of soil were sampled with 10-core bulk samples taken in five repetitions. If plots were contaminated with less than 75 cysts per 100 g of soil, 10-core bulk samples taken in seven repetitions gave cv higher than 23%. This study indicates that more attention should be paid on estimation of sampling error in experimental field plots to ensure more reliable estimation of population density of cyst nematodes.
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Surveillance of Site A and Plot M report for 1991
International Nuclear Information System (INIS)
Golchert, N.W.
1992-05-01
The results of the environmental surveillance program conducted at Site A/Plot M in the Palos Forest Preserve area for CY 1991 are presented. The surveillance program is the ongoing remedial action that resulted from the 1976--1978 radiological characterization of the site. That study determined that very low levels of hydrogen-3 (as tritiated water) had migrated from the burial ground and were present in two nearby hand-pumped picnic wells. The current program consists of sample collection and analysis of air, surface and subsurface water, and bottom sediment. The results of the analyses are used to (1) determine the migration pathway of water from the burial ground (Plot M) to the hand-pumped picnic wells, (2) establish if buried radionuclides other than hydrogen-3 have migrated, and (3) generally characterize the radiological environment of the area. Hydrogen-3 in the Red Gate Woods picnic wells was still detected this year, but the average and maximum concentrations were significantly less than found earlier. Tritiated water continues to be detected in a number of wells, boreholes, dolomite holes, and surface stream. For many years it was the only radionclide found to have migrated in measurable quantities. Analyses since 1984 have indicated the presence of low levels of strontium-90 in water from a number of borehole next to Plot M. The available data does not allow a firm conclusion as to whether the presence of this nuclide represents recent migration or movement that may have occurred before Plot M was capped. The results of the surveillance program continue to indicate that the radioactivity remaining at Site A/Plot M does not endanger the health or safety of the public visiting the site or those living in the vicinity
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Surveillance of Site A and Plot M. Report for 1996
International Nuclear Information System (INIS)
Golchert, N.W.
1997-05-01
The results of the environmental surveillance program conducted at Site A/Plot M in the Palos Forest Preserve area for 1996 are presented. The surveillance program is the ongoing remedial action that resulted from the 1976-1978 radiological characterization of the site. That study determined that very low levels of hydrogen-3 (as tritiated water) had migrated from the burial ground and were present in two nearby hand-pumped picnic wells. The current program consists of sample collection and analysis of air, surface and subsurface water, and bottom sediment. The results of the analyses are used to (1) monitor the migration pathway of water from the burial ground (Plot M) to the hand-pumped picnic wells, (2) establish if buried radionuclides other than hydrogen-3 have migrated, and (3) generally characterize the radiological environment of the area. Hydrogen-3 in the Red Gate Woods picnic wells was still detected this year, but the average and maximum concentrations were significantly less than found earlier. Tritiated water continues to be detected in a number of wells, boreholes, dolomite holes, and a surface stream. For many years it was the only radionuclide found to have migrated in measurable quantities. Analyses since 1984 have indicated the presence of low levels of strontium-90 in water from a number of boreholes next to Plot M. The available data does not allow a firm conclusion as to whether the presence of this nuclide represents recent migration or movement that may have occurred before Plot M was capped. The results of the surveillance program continue to indicate that the radioactivity remaining at Site A/Plot M does not endanger the health or safety of the public visiting the site, using the picnic area, or living in the vicinity
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Mars Science Laboratory Launch-Arrival Space Study: A Pork Chop Plot Analysis
Cianciolo, Alicia Dwyer; Powell, Richard; Lockwood, Mary Kae
2006-01-01
Launch-Arrival, or "pork chop", plot analysis can provide mission designers with valuable information and insight into a specific launch and arrival space selected for a mission. The study begins with the array of entry states for each pair of selected Earth launch and Mars arrival dates, and nominal entry, descent and landing trajectories are simulated for each pair. Parameters of interest, such as maximum heat rate, are plotted in launch-arrival space. The plots help to quickly identify launch and arrival regions that are not feasible under current constraints or technology and also provide information as to what technologies may need to be developed to reach a desired region. This paper provides a discussion of the development, application, and results of a pork chop plot analysis to the Mars Science Laboratory mission. This technique is easily applicable to other missions at Mars and other destinations.
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Variable Selection Strategies for Small-area Estimation Using FIA Plots and Remotely Sensed Data
Andrew Lister; Rachel Riemann; James Westfall; Mike Hoppus
2005-01-01
The USDA Forest Service's Forest Inventory and Analysis (FIA) unit maintains a network of tens of thousands of georeferenced forest inventory plots distributed across the United States. Data collected on these plots include direct measurements of tree diameter and height and other variables. We present a technique by which FIA plot data and coregistered...
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Crop residue decomposition in Minnesota biochar-amended plots
Weyers, S. L.; Spokas, K. A.
2014-06-01
Impacts of biochar application at laboratory scales are routinely studied, but impacts of biochar application on decomposition of crop residues at field scales have not been widely addressed. The priming or hindrance of crop residue decomposition could have a cascading impact on soil processes, particularly those influencing nutrient availability. Our objectives were to evaluate biochar effects on field decomposition of crop residue, using plots that were amended with biochars made from different plant-based feedstocks and pyrolysis platforms in the fall of 2008. Litterbags containing wheat straw material were buried in July of 2011 below the soil surface in a continuous-corn cropped field in plots that had received one of seven different biochar amendments or a uncharred wood-pellet amendment 2.5 yr prior to start of this study. Litterbags were collected over the course of 14 weeks. Microbial biomass was assessed in treatment plots the previous fall. Though first-order decomposition rate constants were positively correlated to microbial biomass, neither parameter was statistically affected by biochar or wood-pellet treatments. The findings indicated only a residual of potentially positive and negative initial impacts of biochars on residue decomposition, which fit in line with established feedstock and pyrolysis influences. Overall, these findings indicate that no significant alteration in the microbial dynamics of the soil decomposer communities occurred as a consequence of the application of plant-based biochars evaluated here.
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Crop residue decomposition in Minnesota biochar amended plots
Weyers, S. L.; Spokas, K. A.
2014-02-01
Impacts of biochar application at laboratory scales are routinely studied, but impacts of biochar application on decomposition of crop residues at field scales have not been widely addressed. The priming or hindrance of crop residue decomposition could have a cascading impact on soil processes, particularly those influencing nutrient availability. Our objectives were to evaluate biochar effects on field decomposition of crop residue, using plots that were amended with biochars made from different feedstocks and pyrolysis platforms prior to the start of this study. Litterbags containing wheat straw material were buried below the soil surface in a continuous-corn cropped field in plots that had received one of seven different biochar amendments or a non-charred wood pellet amendment 2.5 yr prior to start of this study. Litterbags were collected over the course of 14 weeks. Microbial biomass was assessed in treatment plots the previous fall. Though first-order decomposition rate constants were positively correlated to microbial biomass, neither parameter was statistically affected by biochar or wood-pellet treatments. The findings indicated only a residual of potentially positive and negative initial impacts of biochars on residue decomposition, which fit in line with established feedstock and pyrolysis influences. Though no significant impacts were observed with field-weathered biochars, effective soil management may yet have to account for repeat applications of biochar.
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Information extraction from FN plots of tungsten microemitters
Energy Technology Data Exchange (ETDEWEB)
Mussa, Khalil O. [Department of Physics, Mu' tah University, Al-Karak (Jordan); Mousa, Marwan S., E-mail: mmousa@mutah.edu.jo [Department of Physics, Mu' tah University, Al-Karak (Jordan); Fischer, Andreas, E-mail: andreas.fischer@physik.tu-chemnitz.de [Institut für Physik, Technische Universität Chemnitz, Chemnitz (Germany)
2013-09-15
Tungsten based microemitter tips have been prepared both clean and coated with dielectric materials. For clean tungsten tips, apex radii have been varied ranging from 25 to 500 nm. These tips were manufactured by electrochemical etching a 0.1 mm diameter high purity (99.95%) tungsten wire at the meniscus of two molar NaOH solution. Composite micro-emitters considered here are consisting of a tungsten core coated with different dielectric materials—such as magnesium oxide (MgO), sodium hydroxide (NaOH), tetracyanoethylene (TCNE), and zinc oxide (ZnO). It is worthwhile noting here, that the rather unconventional NaOH coating has shown several interesting properties. Various properties of these emitters were measured including current–voltage (IV) characteristics and the physical shape of the tips. A conventional field emission microscope (FEM) with a tip (cathode)–screen (anode) separation standardized at 10 mm was used to electrically characterize the electron emitters. The system was evacuated down to a base pressure of ∼10{sup −8}mbar when baked at up to ∼180°C overnight. This allowed measurements of typical field electron emission (FE) characteristics, namely the IV characteristics and the emission images on a conductive phosphorus screen (the anode). Mechanical characterization has been performed through a FEI scanning electron microscope (SEM). Within this work, the mentioned experimental results are connected to the theory for analyzing Fowler–Nordheim (FN) plots. We compared and evaluated the data extracted from clean tungsten tips of different radii and determined deviations between the results of different extraction methods applied. In particular, we derived the apex radii of several clean and coated tungsten tips by both SEM imaging and analyzing FN plots. The aim of this analysis is to support the ongoing discussion on recently developed improvements of the theory for analyzing FN plots related to metal field electron emitters, which in
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Information extraction from FN plots of tungsten microemitters
International Nuclear Information System (INIS)
Mussa, Khalil O.; Mousa, Marwan S.; Fischer, Andreas
2013-01-01
Tungsten based microemitter tips have been prepared both clean and coated with dielectric materials. For clean tungsten tips, apex radii have been varied ranging from 25 to 500 nm. These tips were manufactured by electrochemical etching a 0.1 mm diameter high purity (99.95%) tungsten wire at the meniscus of two molar NaOH solution. Composite micro-emitters considered here are consisting of a tungsten core coated with different dielectric materials—such as magnesium oxide (MgO), sodium hydroxide (NaOH), tetracyanoethylene (TCNE), and zinc oxide (ZnO). It is worthwhile noting here, that the rather unconventional NaOH coating has shown several interesting properties. Various properties of these emitters were measured including current–voltage (IV) characteristics and the physical shape of the tips. A conventional field emission microscope (FEM) with a tip (cathode)–screen (anode) separation standardized at 10 mm was used to electrically characterize the electron emitters. The system was evacuated down to a base pressure of ∼10 −8 mbar when baked at up to ∼180°C overnight. This allowed measurements of typical field electron emission (FE) characteristics, namely the IV characteristics and the emission images on a conductive phosphorus screen (the anode). Mechanical characterization has been performed through a FEI scanning electron microscope (SEM). Within this work, the mentioned experimental results are connected to the theory for analyzing Fowler–Nordheim (FN) plots. We compared and evaluated the data extracted from clean tungsten tips of different radii and determined deviations between the results of different extraction methods applied. In particular, we derived the apex radii of several clean and coated tungsten tips by both SEM imaging and analyzing FN plots. The aim of this analysis is to support the ongoing discussion on recently developed improvements of the theory for analyzing FN plots related to metal field electron emitters, which in
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Recurrence plot for parameters analysing of internal combustion engine
Alexa, O.; Ilie, C. O.; Marinescu, M.; Vilau, R.; Grosu, D.
2015-11-01
In many technical disciplines modem data analysis techniques has been successfully applied to understand the complexity of the system. The growing volume of theoretical knowledge about systems dynamic's offered researchers the opportunity to look for non-linear dynamics in data whose evolution linear models are unable to explain in a satisfactory manner. One approach in this respect is Recurrence Analysis - RA which is a graphical method designed to locate hidden recurring patterns, nonstationarity and structural changes. RA approach arose in natural sciences like physics and biology but quickly was adopted in economics and engineering. Meanwhile. The fast development of computer resources has provided powerful tools to perform this new and complex model. One free software which was used to perform our analysis is Visual Recurrence Analysis - VRA developed by Eugene Kononov. As is presented in this paper, the recurrence plot investigation for the analyzing of the internal combustion engine shows some of the RPA capabilities in this domain. We chose two specific engine parameters measured in two different tests to perform the RPA. These parameters are injection impulse width and engine angular speed and the tests are I11n and I51n. There were computed graphs for each of them. Graphs were analyzed and compared to obtain a conclusion. This work is an incipient research, being one of the first attempts of using recurrence plot for analyzing automotive dynamics. It opens a wide field of action for future research programs.
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Buma, Brian; Bisbing, Sarah; Krapek, John; Wright, Glenn
2017-06-01
Understanding plant community succession is one of the original pursuits of ecology, forming some of the earliest theoretical frameworks in the field. Much of this was built on the long-term research of William S. Cooper, who established a permanent plot network in Glacier Bay, Alaska, in 1916. This study now represents the longest-running primary succession plot network in the world. Permanent plots are useful for their ability to follow mechanistic change through time without assumptions inherent in space-for-time (chronosequence) designs. After 100-yr, these plots show surprising variety in species composition, soil characteristics (carbon, nitrogen, depth), and percent cover, attributable to variation in initial vegetation establishment first noted by Cooper in the 1916-1923 time period, partially driven by dispersal limitations. There has been almost a complete community composition replacement over the century and general species richness increase, but the effective number of species has declined significantly due to dominance of Salix species which established 100-yr prior (the only remaining species from the original cohort). Where Salix dominates, there is no establishment of "later" successional species like Picea. Plots nearer the entrance to Glacier Bay, and thus closer to potential seed sources after the most recent glaciation, have had consistently higher species richness for 100 yr. Age of plots is the best predictor of soil N content and C:N ratio, though plots still dominated by Salix had lower overall N; soil accumulation was more associated with dominant species. This highlights the importance of contingency and dispersal in community development. The 100-yr record of these plots, including species composition, spatial relationships, cover, and observed interactions between species provides a powerful view of long-term primary succession. © 2017 by the Ecological Society of America.
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Analysis of discrepancies in Dalitz plot parameters in η→3π decay
International Nuclear Information System (INIS)
Kolesár, Marián
2011-01-01
We analyze the Dalitz plot parameters of η→3π decay in the framework of resummed chiral perturbation theory. This approach allows us to keep the uncertainties in the NNLO and higher orders under better control and estimate their influence. We cannot confirm the suspected discrepancy in the case of the charged decay parameter b, where even small uncertainties in higher orders could accommodate the difference. On the other hand, we find the experimental value of the neutral decay parameter α incompatible with an assumption of good convergence properties in the center of the Dalitz plot. We calculate ππ rescattering bubble corrections up to three loops and show that these might explain the discrepancy, especially for a low value of the pseudoscalar decay constant in the chiral limit. However, that could indicate a failure of convergence of the chiral series in this channel already at low energies around 500MeV.
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International Nuclear Information System (INIS)
Schallreuter, Karin U.; Wazir, Umar; Kothari, Sonal; Gibbons, Nicholas C.J.; Moore, Jeremy; Wood, John M.
2004-01-01
Epidermal phenylalanine hydroxylase (PAH) produces L-tyrosine from the essential amino acid L-phenylalanine supporting melanogenesis in human melanocytes. Those PAH activities increase linearly in the different skin phototypes I-VI (Fitzpatrick classification) and also increase up to 24 h after UVB light with only one minimal erythemal dose. Since UVB generates also H 2 O 2 , we here asked the question whether this reactive oxygen species could influence the activity of pure recombinant human PAH. Under saturating conditions with the substrate L-phenylalanine (1 x 10 -3 M), the V max for enzyme activity increased 4-fold by H 2 O 2 (>2.0 x 10 -3 M). Lineweaver-Burk analysis identified a mixed activation mechanism involving both the regulatory and catalytic domains of PAH. Hyperchem molecular modelling and Deep View analysis support oxidation of the single Trp 120 residue to 5-OH-Trp 120 by H 2 O 2 causing a conformational change in the regulatory domain. PAH was still activated by H 2 O 2 in the presence of the electron donor/cofactor 6(R)-L-erythro-5,6,7,8-tetrahydrobiopterin despite slow oxidation of this cofactor. In vivo FT-Raman spectroscopy confirmed decreased epidermal phenylalanine in association with increased tyrosine after UVB exposure. Hence, generation of H 2 O 2 by UVB can activate epidermal PAH leading to an increased L-tyrosine pool for melanogenesis
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Topping, Christopher John; Kjaer, Lene Jung; Hommen, Udo; Høye, Toke Thomas; Preuss, Thomas G; Sibly, Richard M; van Vliet, Peter
2014-07-01
Current European Union regulatory risk assessment allows application of pesticides provided that recovery of nontarget arthropods in-crop occurs within a year. Despite the long-established theory of source-sink dynamics, risk assessment ignores depletion of surrounding populations and typical field trials are restricted to plot-scale experiments. In the present study, the authors used agent-based modeling of 2 contrasting invertebrates, a spider and a beetle, to assess how the area of pesticide application and environmental half-life affect the assessment of recovery at the plot scale and impact the population at the landscape scale. Small-scale plot experiments were simulated for pesticides with different application rates and environmental half-lives. The same pesticides were then evaluated at the landscape scale (10 km × 10 km) assuming continuous year-on-year usage. The authors' results show that recovery time estimated from plot experiments is a poor indicator of long-term population impact at the landscape level and that the spatial scale of pesticide application strongly determines population-level impact. This raises serious doubts as to the utility of plot-recovery experiments in pesticide regulatory risk assessment for population-level protection. Predictions from the model are supported by empirical evidence from a series of studies carried out in the decade starting in 1988. The issues raised then can now be addressed using simulation. Prediction of impacts at landscape scales should be more widely used in assessing the risks posed by environmental stressors. © 2014 SETAC.
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Looking at large data sets using binned data plots
Energy Technology Data Exchange (ETDEWEB)
Carr, D.B.
1990-04-01
This report addresses the monumental challenge of developing exploratory analysis methods for large data sets. The goals of the report are to increase awareness of large data sets problems and to contribute simple graphical methods that address some of the problems. The graphical methods focus on two- and three-dimensional data and common task such as finding outliers and tail structure, assessing central structure and comparing central structures. The methods handle large sample size problems through binning, incorporate information from statistical models and adapt image processing algorithms. Examples demonstrate the application of methods to a variety of publicly available large data sets. The most novel application addresses the too many plots to examine'' problem by using cognostics, computer guiding diagnostics, to prioritize plots. The particular application prioritizes views of computational fluid dynamics solution sets on the fly. That is, as each time step of a solution set is generated on a parallel processor the cognostics algorithms assess virtual plots based on the previous time step. Work in such areas is in its infancy and the examples suggest numerous challenges that remain. 35 refs., 15 figs.
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A user's guide of a plotting program PLTJOINT
International Nuclear Information System (INIS)
Sasaki, Makoto; Sato, Wakaei; Nakagawa, Masayuki; Mori, Takamasa
1988-02-01
A plotting program PLTJOINT is able to plot various types of input and output data from neutronics calculations with two dimensional coordinates. These data include cross sections, neutron flux or spectrum, reaction rate distribution and auxiliary data. These are read from the input and output files of the JOINT system and the transport code system using double differential form cross section, and from arbitrary BCD input file. General purpose option is also available. Much care is taken in lettering such as types of characters (italic, Greek letters and characters), super and subscript of alphabet and numeral. Users can design their own characters by using a program IGDRASIL. Most of input data can be read in free format. The code has been programed so flexibly that new functions can be easily added to. (author)
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International Nuclear Information System (INIS)
Singh, Bhupinder; Diwan, Anubhav; Jain, Varun; Herrera-Gomez, Alberto; Terry, Jeff; Linford, Matthew R.
2016-01-01
Highlights: • Uniqueness plots are introduced as a new tool for identifying poor XPS peak fits. • Uniqueness plots are demonstrated on real XPS data sets. • A horizontal line in a uniqueness plot indicates a poor fit, i.e., fit parameter correlation. • A parabolic shape in a uniqueness plot indicates that a fit may be appropriate. - Abstract: Peak fitting is an essential part of X-ray photoelectron spectroscopy (XPS) narrow scan analysis, and the Literature contains both good and bad examples of peak fitting. A common cause of poor peak fitting is the inclusion of too many fit parameters, often without a sound chemical and/or physical basis for them, and/or the failure to reasonably constrain them. Under these conditions, fit parameters are often correlated, and therefore lacking in statistical meaning. Here we introduce the uniqueness plot as a simple graphical tool for identifying bad peak fits in XPS, i.e., fit parameter correlation. These plots are widely used in spectroscopic ellipsometry. We illustrate uniqueness plots with two data sets: a C 1s narrow scan from ozone-treated carbon nanotube forests and an Si 2p narrow scan from an air-oxidized silicon wafer. For each fit, we consider different numbers of parameters and constraints on them. As expected, the uniqueness plots are parabolic when fewer fit parameters and/or more constraints are applied. However, they fan out and eventually become horizontal lines as more unconstrained parameters are included in the fits. Uniqueness plots are generated by plotting the chi squared (χ 2 ) value for a fit vs. a systematically varied value of a parameter in the fit. The Abbe criterion is also considered as a figure of merit for uniqueness plots in the Supporting Information. We recommend that uniqueness plots be used by XPS practitioners for identifying inappropriate peak fits.
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Energy Technology Data Exchange (ETDEWEB)
Singh, Bhupinder; Diwan, Anubhav; Jain, Varun [Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, 84606 (United States); Herrera-Gomez, Alberto [CINVESTAV-Unidad Queretaro, Queretaro, 76230 (Mexico); Terry, Jeff [Department of Physics, Illinois Institute of Technology, Chicago, IL, 60616 (United States); Linford, Matthew R., E-mail: mrlinford@chem.byu.edu [Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, 84606 (United States)
2016-11-30
Highlights: • Uniqueness plots are introduced as a new tool for identifying poor XPS peak fits. • Uniqueness plots are demonstrated on real XPS data sets. • A horizontal line in a uniqueness plot indicates a poor fit, i.e., fit parameter correlation. • A parabolic shape in a uniqueness plot indicates that a fit may be appropriate. - Abstract: Peak fitting is an essential part of X-ray photoelectron spectroscopy (XPS) narrow scan analysis, and the Literature contains both good and bad examples of peak fitting. A common cause of poor peak fitting is the inclusion of too many fit parameters, often without a sound chemical and/or physical basis for them, and/or the failure to reasonably constrain them. Under these conditions, fit parameters are often correlated, and therefore lacking in statistical meaning. Here we introduce the uniqueness plot as a simple graphical tool for identifying bad peak fits in XPS, i.e., fit parameter correlation. These plots are widely used in spectroscopic ellipsometry. We illustrate uniqueness plots with two data sets: a C 1s narrow scan from ozone-treated carbon nanotube forests and an Si 2p narrow scan from an air-oxidized silicon wafer. For each fit, we consider different numbers of parameters and constraints on them. As expected, the uniqueness plots are parabolic when fewer fit parameters and/or more constraints are applied. However, they fan out and eventually become horizontal lines as more unconstrained parameters are included in the fits. Uniqueness plots are generated by plotting the chi squared (χ{sup 2}) value for a fit vs. a systematically varied value of a parameter in the fit. The Abbe criterion is also considered as a figure of merit for uniqueness plots in the Supporting Information. We recommend that uniqueness plots be used by XPS practitioners for identifying inappropriate peak fits.
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The Zombie Plot: A Simple Graphic Method for Visualizing the Efficacy of a Diagnostic Test.
Richardson, Michael L
2016-08-09
One of the most important jobs of a radiologist is to pick the most appropriate imaging test for a particular clinical situation. Making a proper selection sometimes requires statistical analysis. The objective of this article is to introduce a simple graphic technique, an ROC plot that has been divided into zones of mostly bad imaging efficacy (ZOMBIE, hereafter referred to as the "zombie plot"), that transforms information about imaging efficacy from the numeric domain into the visual domain. The numeric rationale for the use of zombie plots is given, as are several examples of the clinical use of these plots. Two online calculators are described that simplify the process of producing a zombie plot.
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D-GENIES: dot plot large genomes in an interactive, efficient and simple way.
Cabanettes, Floréal; Klopp, Christophe
2018-01-01
Dot plots are widely used to quickly compare sequence sets. They provide a synthetic similarity overview, highlighting repetitions, breaks and inversions. Different tools have been developed to easily generated genomic alignment dot plots, but they are often limited in the input sequence size. D-GENIES is a standalone and web application performing large genome alignments using minimap2 software package and generating interactive dot plots. It enables users to sort query sequences along the reference, zoom in the plot and download several image, alignment or sequence files. D-GENIES is an easy-to-install, open-source software package (GPL) developed in Python and JavaScript. The source code is available at https://github.com/genotoul-bioinfo/dgenies and it can be tested at http://dgenies.toulouse.inra.fr/.
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EcoIS: An image serialization library for plot-based plant flowering phenology
DEFF Research Database (Denmark)
Granados, Joel A.; Bonnet, Philippe; Hansen, Lars Hostrup
2013-01-01
they are produced by introducing an open source Python (www.python.org) library called EcoIS that creates image series from unaligned pictures of specially equipped plots. We use EcoIS to sample flowering phenology plots in a high arctic environment and create image series that later generate phenophase counts...
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READING COLONIZATION IN CONRAD'S TRANS-RACIAL LOVE PLOTS
Directory of Open Access Journals (Sweden)
Sandra Lilyana
2017-07-01
Full Text Available The study is about a close look at Conrad's trans-racial romance related to the Victorian period. Trans-racial love between white men and non-white women becomes a popular theme in the early works of Josep Conrad, a famous writer of the late Victorian period. Using a closely technical reading in the three of Conrad's works Lord Jim, Almayer's Folly, and An Outcast of the Island, we can show that such a trans-racial romance is not merely meant for appreciating equivalence. In turn, the trans-racial romance of Conrad's can be understood as the reflection of the Western colonization on the East where the white men take a role as subjects who had dominately explored while the non-white women as objects who are passively being explored. Key words: colonization, race, romance plot, subject, object, and dominance
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Fitting diameter distribution models to data from forest inventories with concentric plot design
Directory of Open Access Journals (Sweden)
Nikos Nanos
2017-10-01
Research highlights:We designed a new method to fit the Weibull distribution to forest inventory data from concentric plots that achieves high accuracy and precision in parameter estimates regardless of the within-plot spatial tree pattern.
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The hexagon/panel system for selecting FIA plots under an annual inventory
Gary J. Brand; Mark D. Nelson; Daniel G. Wendt; Kevin K. Nimerfro
2000-01-01
Forest Inventory and Analysis (FIA) is changing to an annual nationwide forest inventory. This paper describes the sampling grid used to distribute FIA plots across the landscape and to allocate them to a particular measurement year. We also describe the integration of the F1A and Forest Health Monitoring (FHM) plot networks.
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Automatic Classification of Station Quality by Image Based Pattern Recognition of Ppsd Plots
Weber, B.; Herrnkind, S.
2017-12-01
The number of seismic stations is growing and it became common practice to share station waveform data in real-time with the main data centers as IRIS, GEOFON, ORFEUS and RESIF. This made analyzing station performance of increasing importance for automatic real-time processing and station selection. The value of a station depends on different factors as quality and quantity of the data, location of the site and general station density in the surrounding area and finally the type of application it can be used for. The approach described by McNamara and Boaz (2006) became standard in the last decade. It incorporates a probability density function (PDF) to display the distribution of seismic power spectral density (PSD). The low noise model (LNM) and high noise model (HNM) introduced by Peterson (1993) are also displayed in the PPSD plots introduced by McNamara and Boaz allowing an estimation of the station quality. Here we describe how we established an automatic station quality classification module using image based pattern recognition on PPSD plots. The plots were split into 4 bands: short-period characteristics (0.1-0.8 s), body wave characteristics (0.8-5 s), microseismic characteristics (5-12 s) and long-period characteristics (12-100 s). The module sqeval connects to a SeedLink server, checks available stations, requests PPSD plots through the Mustang service from IRIS or PQLX/SQLX or from GIS (gempa Image Server), a module to generate different kind of images as trace plots, map plots, helicorder plots or PPSD plots. It compares the image based quality patterns for the different period bands with the retrieved PPSD plot. The quality of a station is divided into 5 classes for each of the 4 bands. Classes A, B, C, D define regular quality between LNM and HNM while the fifth class represents out of order stations with gain problems, missing data etc. Over all period bands about 100 different patterns are required to classify most of the stations available on the
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The PLOT (Paleolimnological Transect) Project in the Russian Arctic
Gromig, R.; Andreev, A.; Baumer, M.; Bolshiyanov, D.; Fedorov, G.; Frolova, L.; Krastel, S.; Lebas, E.; Ludikova, A.; Melles, M.; Meyer, H.; Nazarova, L.; Pestryakova, L.; Savelieva, L.; Shumilovskikh, L.; Subetto, D.; Wagner, B.; Wennrich, V.
2017-12-01
The joint Russian- German project 'PLOT - Paleolimnological Transec' aims to recover lake sediment sequences along a >6000 km long longitudinal transect across the Eurasian Arctic in order to investigate the Late Quaternary climatic and environmental history. The climate history of the Arctic is of particular interest since it is the region, which is experiencing major impact of the current climate change. The project is funded for three years (2015-2018) by the Russian and German Ministries of Research. Since 2013 extensive fieldwork, including seismic surveys, coring, and hydrological investigations, was carried out at lakes Ladoga (NW Russia, pilot study), Bolshoye Shuchye (Polar Urals), Emanda (Verkhoyansk Range, field campaign planned for August 2017), Levinson-Lessing and Taymyr (Taymyr Peninsula). Fieldwork at lakes Bolshoye Shuchye, Levinson-Lessing and Taymyr was conducted in collaboration with the Russian-Norwegian CHASE (Climate History along the Arctic Seaboard of Eurasia) project. A major objective of the PLOT project was to recover preglacial sediments. A multiproxy approach was applied to the analytical work of all cores, including (bio-)geochemical, sedimentological, geophysical, and biological analyses. First data implies the presence of preglacial sediments in the cores from all lakes so far visited. Age-depth models, based on radiocarbon dating, OSL dating, paleomagnetic measurements, identification of cryptotephra, and varve counting (where applicable), are in progress. Climate variability in the records shall be compared to that recorded at Lake Eĺgygytgyn (NE Russia), which represents the master record for the Siberian Arctic. The outcome of the PLOT project will be a better understanding of the temporal and spatial variability and development of the Arctic climate. Here, we present the major results and first key interpretations of the PLOT project, along with an outlook on the future strategy and foci. First results from lakes Ladoga
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The Chymistry of "The Learned Dr Plot" (1640-96).
Roos, Anna Marie
2014-01-01
In the seventeenth century, there were developing norms of openness in the presentation of scientific knowledge that were at odds with traditions of secrecy among chymists, particularly practitioners of chrysopoeia, or the transmutation of metals. This chapter analyzes how Dr. Robert Plot, the first professor of chymistry at Oxford, negotiated these boundaries within an institutional context. I first delineate his chymical and experimental practice, which incorporated procedures from medieval alchemical sources, particularly the Lullian corpus, as well as more novel practices from seventeenth-century chymistry. Then, I analyze how personal and institutional ambitions and economic considerations shaped to what extent Plot negotiated the boundaries between secrecy and the public dissemination of chymical knowledge.
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[Murderers in white coats--the physicians' plot against Stalin].
Olsen, B
1997-12-10
During the Moscow trials in 1936-38 several doctors were sentenced for participating in the murders of party leaders. As others who were accused, the doctors willingly confessed to crimes they could not possibly have committed. A second doctors' plot took place in the autumn of 1952. This time nine doctors, six of them Jews, were arrested for the attempted murder of political leaders. Only the unexpected death of Josef Stalin prevented a new wave of terror. With the single exception of Genrikh Grigorjevitsj Jagoda (1891-1938), head of the NKVD (KGB's predecessor), all those sentenced under the Moscow process are now rehabilitated. The participants of the second doctors' plot were rehabilitated immediately after Stalin's death.
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Robust reconstruction of a signal from its unthresholded recurrence plot subject to disturbances
Energy Technology Data Exchange (ETDEWEB)
Sipers, Aloys, E-mail: aloys.sipers@zuyd.nl [Department of Bèta Sciences and Technology, Zuyd University (Netherlands); Borm, Paul, E-mail: paul.borm@zuyd.nl [Department of Bèta Sciences and Technology, Zuyd University (Netherlands); Peeters, Ralf, E-mail: ralf.peeters@maastrichtuniversity.nl [Department of Data Science and Knowledge Engineering, Maastricht University (Netherlands)
2017-02-12
To make valid inferences from recurrence plots for time-delay embedded signals, two underlying key questions are: (1) to what extent does an unthresholded recurrence (URP) plot carry the same information as the signal that generated it, and (2) how does the information change when the URP gets distorted. We studied the first question in our earlier work , where it was shown that the URP admits the reconstruction of the underlying signal (up to its mean and a choice of sign) if and only if an associated graph is connected. Here we refine this result and we give an explicit condition in terms of the embedding parameters and the discrete Fourier spectrum of the URP. We also develop a method for the reconstruction of the underlying signal which overcomes several drawbacks that earlier approaches had. To address the second question we investigate robustness of the proposed reconstruction method under disturbances. We carry out two simulation experiments which are characterized by a broad band and a narrow band spectrum respectively. For each experiment we choose a noise level and two different pairs of embedding parameters. The conventional binary recurrence plot (RP) is obtained from the URP by thresholding and zero-one conversion, which can be viewed as severe distortion acting on the URP. Typically the reconstruction of the underlying signal from an RP is expected to be rather inaccurate. However, by introducing the concept of a multi-level recurrence plot (MRP) we propose to bridge the information gap between the URP and the RP, while still achieving a high data compression rate. We demonstrate the working of the proposed reconstruction procedure on MRPs, indicating that MRPs with just a few discretization levels can usually capture signal properties and morphologies more accurately than conventional RPs. - Highlights: • A recurrence plot is maximally informative if and only if the corresponding graph is connected. • The diameter of the connected graph is always
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Reproducibility Between Brain Uptake Ratio Using Anatomic Standardization and Patlak-Plot Methods.
Shibutani, Takayuki; Onoguchi, Masahisa; Noguchi, Atsushi; Yamada, Tomoki; Tsuchihashi, Hiroko; Nakajima, Tadashi; Kinuya, Seigo
2015-12-01
The Patlak-plot and conventional methods of determining brain uptake ratio (BUR) have some problems with reproducibility. We formulated a method of determining BUR using anatomic standardization (BUR-AS) in a statistical parametric mapping algorithm to improve reproducibility. The objective of this study was to demonstrate the inter- and intraoperator reproducibility of mean cerebral blood flow as determined using BUR-AS in comparison to the conventional-BUR (BUR-C) and Patlak-plot methods. The images of 30 patients who underwent brain perfusion SPECT were retrospectively used in this study. The images were reconstructed using ordered-subset expectation maximization and processed using an automatic quantitative analysis for cerebral blood flow of ECD tool. The mean SPECT count was calculated from axial basal ganglia slices of the normal side (slices 31-40) drawn using a 3-dimensional stereotactic region-of-interest template after anatomic standardization. The mean cerebral blood flow was calculated from the mean SPECT count. Reproducibility was evaluated using coefficient of variation and Bland-Altman plotting. For both inter- and intraoperator reproducibility, the BUR-AS method had the lowest coefficient of variation and smallest error range about the Bland-Altman plot. Mean CBF obtained using the BUR-AS method had the highest reproducibility. Compared with the Patlak-plot and BUR-C methods, the BUR-AS method provides greater inter- and intraoperator reproducibility of cerebral blood flow measurement. © 2015 by the Society of Nuclear Medicine and Molecular Imaging, Inc.
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Surveillance of Site A and Plot M, Report for 2008.
Energy Technology Data Exchange (ETDEWEB)
Golchert, N. W.
2009-05-07
The results of the environmental surveillance program conducted at Site A/Plot M in the Palos Forest Preserve area for Calendar Year 2008 are presented. Based on the results of the 1976-1978 radiological characterization of the site, a determination was made that a surveillance program be established. The characterization study determined that very low levels of hydrogen-3 (as tritiated water) had migrated from the burial ground and were present in two nearby hand pumped picnic wells. The current surveillance program began in 1980 and consists of sample collection and analysis of surface and subsurface water. The results of the analyses are used to (1) monitor the migration pathway of hydrogen-3 contaminated water from the burial ground (Plot M) to the hand-pumped picnic wells, (2) establish if other buried radionuclides have migrated, and (3) monitor for the presence of radioactive materials in the environment of the area. Hydrogen-3 in the Red Gate Woods picnic wells was still detected this year, but the average and maximum concentrations were significantly less than found earlier. Hydrogen-3 continues to be detected in a number of wells, boreholes, dolomite holes, and a surface stream. Analyses since 1984 have indicated the presence of low levels of strontium-90 in water from a number of boreholes next to Plot M. The results of the surveillance program continue to indicate that the radioactivity remaining at Site A/Plot M does not endanger the health or safety of the public visiting the site, using the picnic area, or living in the vicinity.
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Surveillance of Site A and Plot M, Report for 2009.
Energy Technology Data Exchange (ETDEWEB)
Golchert, N. W.
2010-04-21
The results of the environmental surveillance program conducted at Site A/Plot M in the Palos Forest Preserve area for Calendar Year 2009 are presented. Based on the results of the 1976-1978 radiological characterization of the site, a determination was made that a surveillance program be established. The characterization study determined that very low levels of hydrogen-3 (as tritiated water) had migrated from the burial ground and were present in two nearby hand-pumped picnic wells. The current surveillance program began in 1980 and consists of sample collection and analysis of surface and subsurface water. The results of the analyses are used to monitor the migration pathway of hydrogen-3 contaminated water from the burial ground (Plot M) to the hand-pumped picnic wells and monitor for the presence of radioactive materials in the environment of the area. Hydrogen-3 in the Red Gate Woods picnic wells was still detected this year, but the average and maximum concentrations were significantly less than found earlier. Hydrogen-3 continues to be detected in a number of wells, boreholes, dolomite holes, and a surface stream. Analyses since 1984 have indicated the presence of low levels of strontium-90 in water from a number of boreholes next to Plot M. The results of the surveillance program continue to indicate that the radioactivity remaining at Site A/Plot M does not endanger the health or safety of the public visiting the site, using the picnic area, or living in the vicinity.
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Surveillance of Site A and Plot M report for 2010.
Energy Technology Data Exchange (ETDEWEB)
Golchert, N. W. (ESQ)
2011-05-31
The results of the environmental surveillance program conducted at Site A/Plot M in the Palos Forest Preserve area for Calendar Year 2010 are presented. Based on the results of the 1976-1978 radiological characterization of the site, a determination was made that a surveillance program be established. The characterization study determined that very low levels of hydrogen-3 (as tritiated water) had migrated from the burial ground and were present in two nearby hand-pumped picnic wells. The current surveillance program began in 1980 and consists of sample collection and analysis of surface and subsurface water. The results of the analyses are used to monitor the migration pathway of hydrogen-3 contaminated water from the burial ground (Plot M) to the hand-pumped picnic wells and monitor for the presence of radioactive materials in the environment of the area. Hydrogen-3 in the Red Gate Woods picnic wells was still detected this year, but the average and maximum concentrations were significantly less than found earlier. Hydrogen-3 continues to be detected in a number of wells, boreholes, dolomite holes, and a surface stream. Analyses since 1984 have indicated the presence of low levels of strontium-90 in water from a number of boreholes next to Plot M. The results of the surveillance program continue to indicate that the radioactivity remaining at Site A/Plot M does not endanger the health or safety of the public visiting the site, using the picnic area, or living in the vicinity.
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Surveillance of site A and plot M, report for 2007.
Energy Technology Data Exchange (ETDEWEB)
Golchert, N. W.; ESH/QA Oversight
2008-03-25
The results of the environmental surveillance program conducted at Site A/Plot M in the Palos Forest Preserve area for Calendar Year 2007 are presented. Based on the results of the 1976-1978 radiological characterization of the site, a determination was made that a surveillance program be established. The characterization study determined that very low levels of hydrogen-3 (as tritiated water) had migrated from the burial ground and were present in two nearby hand pumped picnic wells. The current surveillance program began in 1980 and consists of sample collection and analysis of surface and subsurface water. The results of the analyses are used to: (1) monitor the migration pathway of water from the burial ground (Plot M) to the hand-pumped picnic wells, (2) establish if other buried radionuclides have migrated, and (3) monitor the presence of radioactive materials in the environment of the area. Hydrogen-3 in the Red Gate Woods picnic wells was still detected this year, but the average and maximum concentrations were significantly less than found earlier. Hydrogen-3 continues to be detected in a number of wells, boreholes, dolomite holes, and a surface stream. Analyses since 1984 have indicated the presence of low levels of strontium-90 in water from a number of boreholes next to Plot M. The results of the surveillance program continue to indicate that the radioactivity remaining at Site A/Plot M does not endanger the health or safety of the public visiting the site, using the picnic area, or living in the vicinity.
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Surveillance of Site A and Plot M - Report for 2006.
Energy Technology Data Exchange (ETDEWEB)
Golchert, N. W.; ESH/QA Oversight
2007-05-07
The results of the environmental surveillance program conducted at Site A/Plot M in the Palos Forest Preserve area for Calendar Year 2006 are presented. Based on the results of the 1976-1978 radiological characterization of the site, a determination was made that a surveillance program be established. The characterization study determined that very low levels of hydrogen-3 (as tritiated water) had migrated from the burial ground and were present in two nearby hand-pumped picnic wells. The current surveillance program began in 1980 and consists of sample collection and analysis of surface and subsurface water. The results of the analyses are used to (1) monitor the migration pathway of water from the burial ground (PlotM) to the hand pumped picnic wells, (2) establish if buried radionuclides other than hydrogen-3 have migrated, and (3) monitor the presence of radioactive and chemically hazardous materials in the environment of the area. Hydrogen-3 in the Red GateWoods picnic wells was still detected this year, but the average and maximum concentrations were significantly less than found earlier. Hydrogen-3 continues to be detected in a number of wells, boreholes, dolomite holes, and a surface stream. Analyses since 1984 have indicated the presence of low levels of strontium-90 in water from a number of boreholes next to Plot M. The results of the surveillance program continue to indicate that the radioactivity remaining at Site A/Plot M does not endanger the health or safety of the public visiting the site, using the picnic area, or living in the vicinity.
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Surveillance of Site A and Plot M - Report for 2005.
Energy Technology Data Exchange (ETDEWEB)
Golchert, N. W.; ESH/QA Oversight
2006-04-10
The results of the environmental surveillance program conducted at Site A/Plot M in the Palos Forest Preserve area for Calendar Year 2005 are presented. Based on the results of the 1976-1978 radiological characterization of the site, a determination was made that a surveillance program be established. The characterization study determined that very low levels of hydrogen-3 (as tritiated water) had migrated from the burial ground and were present in two nearby handpumped picnic wells. The current surveillance program began in 1980 and consists of sample collection and analysis of surface and subsurface water. The results of the analyses are used to (1) monitor the migration pathway of water from the burial ground (Plot M) to the handpumped picnic wells, (2) establish if buried radionuclides other than hydrogen-3 have migrated, and (3) monitor the presence of radioactive and chemically hazardous materials in the environment of the area. Hydrogen-3 in the Red Gate Woods picnic wells was still detected this year, but the average and maximum concentrations were significantly less than found earlier. Hydrogen-3 continues to be detected in a number of wells, boreholes, dolomite holes, and a surface stream. Analyses since 1984 have indicated the presence of low levels of strontium-90 in water from a number of boreholes next to Plot M. The results of the surveillance program continue to indicate that the radioactivity remaining at Site A/Plot M does not endanger the health or safety of the public visiting the site, using the picnic area, or living in the vicinity.
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D.3.2 PLOT Persuasive Learning Design Framework
DEFF Research Database (Denmark)
Gram-Hansen, Sandra Burri; Schärfe, Henrik; Winther-Nielsen, Nicolai
2011-01-01
of the technological learning tools and products which are currently related to the PLOT project, namely the GLOMaker and the 3ET tool, and a selection of GLOs and learning exercises. The primary focus of the analysis is to explore how the theoretical perspectives presented in D.3.1 are represented in these tools...... as an: ‚Internal report for project use containing an empirically-based assessment of existing systems and their potential in terms of learning and persuasion. This will be used as a discussion document by the consortium.‛ To meet the requirements of this deliverable, this documents presents analysis......, in particular the notions of persuasive design and constructive alignment. Whilst the report provides a persuasive design perspective on the technologies related to Euro PLOT, it must be stressed that if the document is to function as a basis for further discussion within the consortium, the partners...
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CADASTRAL CLASSIFICATION OF THE LAND PLOTS IN UKRAINE
Directory of Open Access Journals (Sweden)
KIRICHEK Yu. O.
2016-04-01
Full Text Available Summary. Work concerns development of national system of classification of the land plots. The developed classification will allow to solve correctly a number of the corresponding cadastral, land management, estimated and other tasks. The analysis of classifications of lands, improvements and real estate in general is made. The created offers concerning creation of a new classification of the land plots in Ukraine. Today the Ukrainian real estate market has no single system that separates the system property groups, classes and types. This significantly complicates the work and can not fully be aware of the specific situation of real estate market. This task is designed to solve classification properties, it is used to transition from a diversity of individual properties to a limited number of classes of evaluation objects. The classification is different functional purpose (use facilities assessment, which determines the difference in value.
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Persuasive Technology for Learning and Teaching – The EuroPLOT Project
Behringer, R; Soosay, M; Gram-Hansen, SB; Øhrstrøm, P; Sørensen, CG; Smith, C; Mikulecká, J; Winther-Nielsen, N; Winther-Nielsen, M; Herber, E
2013-01-01
The concept of persuasive design has demonstrated its benefits by changing human behavior in certain situations, but in the area of education and learning, this approach has rarely been used. To change this and to study the feasibility of persuasive technology in teaching and learning, the EuroPLOT project (PLOT = Persuasive Learning Objects and Technologies) has been funded 2010-2013 by the Education, Audiovisual and Culture Executive Agency (EACEA) in the Life-long Learning (LLL) programme....
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Erickson, David L.; Jones, Frank A.; Swenson, Nathan G.; Pei, Nancai; Bourg, Norman A.; Chen, Wenna; Davies, Stuart J.; Ge, Xue-jun; Hao, Zhanqing; Howe, Robert W.; Huang, Chun-Lin; Larson, Andrew J.; Lum, Shawn K. Y.; Lutz, James A.; Ma, Keping; Meegaskumbura, Madhava; Mi, Xiangcheng; Parker, John D.; Fang-Sun, I.; Wright, S. Joseph; Wolf, Amy T.; Ye, W.; Xing, Dingliang; Zimmerman, Jess K.; Kress, W. John
2014-01-01
Forest dynamics plots, which now span longitudes, latitudes, and habitat types across the globe, offer unparalleled insights into the ecological and evolutionary processes that determine how species are assembled into communities. Understanding phylogenetic relationships among species in a community has become an important component of assessing assembly processes. However, the application of evolutionary information to questions in community ecology has been limited in large part by the lack of accurate estimates of phylogenetic relationships among individual species found within communities, and is particularly limiting in comparisons between communities. Therefore, streamlining and maximizing the information content of these community phylogenies is a priority. To test the viability and advantage of a multi-community phylogeny, we constructed a multi-plot mega-phylogeny of 1347 species of trees across 15 forest dynamics plots in the ForestGEO network using DNA barcode sequence data (rbcL, matK, and psbA-trnH) and compared community phylogenies for each individual plot with respect to support for topology and branch lengths, which affect evolutionary inference of community processes. The levels of taxonomic differentiation across the phylogeny were examined by quantifying the frequency of resolved nodes throughout. In addition, three phylogenetic distance (PD) metrics that are commonly used to infer assembly processes were estimated for each plot [PD, Mean Phylogenetic Distance (MPD), and Mean Nearest Taxon Distance (MNTD)]. Lastly, we examine the partitioning of phylogenetic diversity among community plots through quantification of inter-community MPD and MNTD. Overall, evolutionary relationships were highly resolved across the DNA barcode-based mega-phylogeny, and phylogenetic resolution for each community plot was improved when estimated within the context of the mega-phylogeny. Likewise, when compared with phylogenies for individual plots, estimates of
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Radiological site assessment at sun rose claim utilizing ScanPlot{sup SM} technology
Energy Technology Data Exchange (ETDEWEB)
Downey, H., E-mail: heath.downey@amecfw.com [Amec Foster Wheeler, Portland, ME (United States)
2015-07-01
ScanPlot{sup SM} gamma spectroscopy land survey system was utilized for the overland survey of uranium at the Sun Rose Claim in the Northwest Territories. The Sun Rose Claim is a former uranium exploration site and previous investigations had identified uranium ore and waste rock. ScanPlot{sup SM} radiological scan surveys were performed utilizing a backpack system. ScanPlot{sup SM} platform utilized spectroscopy grade sodium iodide detectors configured for optimal spatial coverage and radiation detection. Survey locations were recorded using an on-board global positioning system (GPS). The radiological spectral data from the radiation detectors is automatically logged and linked with the GPS coordinates to an on-board computer to create isocontour figures using a color scale to represent radioactivity levels. The advantage of utilizing the ScanPlot{sup SM} system for this assessment is that the nature and extent of uranium is provided without having to collect and assay a large number of samples. (author)
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Estimation of Alcohol Concentration of Red Wine Based on Cole-Cole Plot
Watanabe, Kota; Taka, Yoshinori; Fujiwara, Osamu
To evaluate the quality of wine, we previously measured the complex relative permittivity of wine in the frequency range from 10 MHz to 6 GHz with a network analyzer, and suggested a possibility that the maturity and alcohol concentration of wine can simultaneously be estimated from the Cole-Cole plot. Although the absolute accuracy has not been examined yet, this method will enable one to estimate the alcohol concentration of alcoholic beverages without any distillation equipment simply. In this study, to investigate the estimation accuracy of the alcohol concentration of wine by its Cole-Cole plots, we measured the complex relative permittivity of pure water and diluted ethanol solution from 100 MHz to 40 GHz, and obtained the dependence of the Cole-Cole plot parameters on alcohol concentration and temperature. By using these results as calibration data, we estimated the alcohol concentration of red wine from the Cole-Cole plots, which was compared with the measured one based on a distillation method. As a result, we have confirmed that the estimated alcohol concentration of red wine agrees with the measured results in an absolute error by less than 1 %.
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Construction of a Simple Low-Cost Teslameter and Its Use with Arduino and MakerPlot Software
Atkin, Keith
2016-01-01
This paper shows how it is possible to construct a very simple device for the measurement of magnetic flux densities in an educational context. It is also shown how such a device can be interfaced to a microcontroller with plotting-software to facilitate the study of magnetic fields produced by a current-carrying coil.
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Energy Technology Data Exchange (ETDEWEB)
Cleland, Timothy James [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
2018-01-02
The Los Alamos National Laboratory Plotting Software for the Nuclear Weapons Analysis Tools is a Java™ application based upon the open source library JFreeChart. The software provides a capability for plotting data on graphs with a rich variety of display options while allowing the viewer interaction via graph manipulation and scaling to best view the data. The graph types include XY plots, Date XY plots, Bar plots and Histogram plots.
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Constructing General Orthogonal Fractional Factorial Split-Plot Designs
Sartono, B.; Goos, P.; Schoen, E.
2015-01-01
While the orthogonal design of split-plot fractional factorial experiments has received much attention already, there are still major voids in the literature. First, designs with one or more factors acting at more than two levels have not yet been considered. Second, published work on nonregular
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Enhancement to the Tektronix PLOT-10 Terminal Control System for creation of graphics metafiles
International Nuclear Information System (INIS)
Gray, W.H.
1983-01-01
Many data handling and analysis codes at the Oak Ridge National Laboratory (ORNL) use the Tektronix PLOT-10 Terminal Control System to graphically display data upon Tektronix or Tektronix-emulating graphics devices. Prior to the development of the software libraries and postprocessors discussed within this report, ORNL users were limited to the type of hardcopy output obtainable from the Tektronix PLOT-10 software library. Only Tektronix graphics devices are supported by the PLOT-10 library. The graphics library presented here eliminates this restriction by implementing a suite of software that optionally creates a graphics metafile within the user's disk area while simultaneously drawing a display image on the screen of a user's Tektronix terminal. This graphics metafile can then be postprocessed onto any of the graphics devices at ORNL via the ORNL PLOT command
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Greenslade, Thomas B., Jr.
2018-01-01
Some of you may remember the 1979 television series "Connections" that was written and narrated by James Burke, a British science writer. Burke's technique was to choose a number of seemingly unrelated ideas and show how they led to developments in science and technology. This is an enjoyable business, even if some of the connections…
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Swallowing sound detection using hidden markov modeling of recurrence plot features
International Nuclear Information System (INIS)
Aboofazeli, Mohammad; Moussavi, Zahra
2009-01-01
Automated detection of swallowing sounds in swallowing and breath sound recordings is of importance for monitoring purposes in which the recording durations are long. This paper presents a novel method for swallowing sound detection using hidden Markov modeling of recurrence plot features. Tracheal sound recordings of 15 healthy and nine dysphagic subjects were studied. The multidimensional state space trajectory of each signal was reconstructed using the Taken method of delays. The sequences of three recurrence plot features of the reconstructed trajectories (which have shown discriminating capability between swallowing and breath sounds) were modeled by three hidden Markov models. The Viterbi algorithm was used for swallowing sound detection. The results were validated manually by inspection of the simultaneously recorded airflow signal and spectrogram of the sounds, and also by auditory means. The experimental results suggested that the performance of the proposed method using hidden Markov modeling of recurrence plot features was superior to the previous swallowing sound detection methods.
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Swallowing sound detection using hidden markov modeling of recurrence plot features
Energy Technology Data Exchange (ETDEWEB)
Aboofazeli, Mohammad [Faculty of Engineering, Department of Electrical and Computer Engineering, University of Manitoba, Winnipeg, Manitoba, R3T 5V6 (Canada)], E-mail: umaboofa@cc.umanitoba.ca; Moussavi, Zahra [Faculty of Engineering, Department of Electrical and Computer Engineering, University of Manitoba, Winnipeg, Manitoba, R3T 5V6 (Canada)], E-mail: mousavi@ee.umanitoba.ca
2009-01-30
Automated detection of swallowing sounds in swallowing and breath sound recordings is of importance for monitoring purposes in which the recording durations are long. This paper presents a novel method for swallowing sound detection using hidden Markov modeling of recurrence plot features. Tracheal sound recordings of 15 healthy and nine dysphagic subjects were studied. The multidimensional state space trajectory of each signal was reconstructed using the Taken method of delays. The sequences of three recurrence plot features of the reconstructed trajectories (which have shown discriminating capability between swallowing and breath sounds) were modeled by three hidden Markov models. The Viterbi algorithm was used for swallowing sound detection. The results were validated manually by inspection of the simultaneously recorded airflow signal and spectrogram of the sounds, and also by auditory means. The experimental results suggested that the performance of the proposed method using hidden Markov modeling of recurrence plot features was superior to the previous swallowing sound detection methods.
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Directory of Open Access Journals (Sweden)
Li MingChu
2017-01-01
Full Text Available The terrorist’s coordinated attack is becoming an increasing threat to western countries. By monitoring potential terrorists, security agencies are able to detect and destroy terrorist plots at their planning stage. Therefore, an optimal monitoring strategy for the domestic security agency becomes necessary. However, previous study about monitoring strategy generation fails to consider the information leakage, due to hackers and insider threat. Such leakage events may lead to failure of watching potential terrorists and destroying the plot, and cause a huge risk to public security. This paper makes two major contributions. Firstly, we develop a new Stackelberg game model for the security agency to generate optimal monitoring strategy with the consideration of information leakage. Secondly, we provide a double-oracle framework DO-TPDIL for calculation effectively. The experimental result shows that our approach can obtain robust strategies against information leakage with high feasibility and efficiency.
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On the role of final-state interactions in Dalitz plot studies
International Nuclear Information System (INIS)
Kubis, Bastian; Niecknig, Franz; Schneider, Sebastian P.
2012-01-01
The study of Dalitz plots of heavy-meson decays to multi-hadron final states has received intensified interest by the possibility to gain access to precision investigations of CP violation. A thorough understanding of the hadronic final-state interactions is a prerequisite to achieve a highly sensitive, model-independent study of such Dalitz plots. We illustrate some of the theoretical tools, predominantly taken from dispersion theory, available for these and related purposes, and discuss the low-energy decays ω,φ→3π in some more detail.
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On the role of final-state interactions in Dalitz plot studies
Energy Technology Data Exchange (ETDEWEB)
Kubis, Bastian, E-mail: kubis@hiskp.uni-bonn.de [Helmholtz-Institut fuer Strahlen- und Kernphysik and Bethe Center for Theoretical Physics, Universitaet Bonn (Germany); Niecknig, Franz; Schneider, Sebastian P. [Helmholtz-Institut fuer Strahlen- und Kernphysik and Bethe Center for Theoretical Physics, Universitaet Bonn (Germany)
2012-04-15
The study of Dalitz plots of heavy-meson decays to multi-hadron final states has received intensified interest by the possibility to gain access to precision investigations of CP violation. A thorough understanding of the hadronic final-state interactions is a prerequisite to achieve a highly sensitive, model-independent study of such Dalitz plots. We illustrate some of the theoretical tools, predominantly taken from dispersion theory, available for these and related purposes, and discuss the low-energy decays {omega},{phi}{yields}3{pi} in some more detail.
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On the role of final-state interactions in Dalitz plot studies
Kubis, Bastian; Niecknig, Franz; Schneider, Sebastian P.
2012-04-01
The study of Dalitz plots of heavy-meson decays to multi-hadron final states has received intensified interest by the possibility to gain access to precision investigations of CP violation. A thorough understanding of the hadronic final-state interactions is a prerequisite to achieve a highly sensitive, model-independent study of such Dalitz plots. We illustrate some of the theoretical tools, predominantly taken from dispersion theory, available for these and related purposes, and discuss the low-energy decays ω,ϕ→3π in some more detail.
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Graphical interpretation of confidence curves in rankit plots
DEFF Research Database (Denmark)
Hyltoft Petersen, Per; Blaabjerg, Ole; Andersen, Marianne
2004-01-01
A well-known transformation from the bell-shaped Gaussian (normal) curve to a straight line in the rankit plot is investigated, and a tool for evaluation of the distribution of reference groups is presented. It is based on the confidence intervals for percentiles of the calculated Gaussian distri...
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Geopolitical location and plot in The Night Manager
DEFF Research Database (Denmark)
Agger, Gunhild
2017-01-01
The Night Manager. Three perspectives are tested in combining the analysis of location with the development of the plot: the general concepts of universal and real geography; the idea of being located inside or outside a given context; and the associated notion of centre-periphery opposition. A geopolitical...
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DEFF Research Database (Denmark)
Sidiropoulos, Nikos; Sohi, Sina Hadi; Pedersen, Thomas Lin
2017-01-01
the representation of data sets with differing sample size we have developed a new type of plot overcoming limitations of current standard visualization charts. SinaPlot is inspired by the strip chart and the violin plot and operates by letting the normalized density of points restrict the jitter along the x...
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Singh, Bhupinder; Diwan, Anubhav; Jain, Varun; Herrera-Gomez, Alberto; Terry, Jeff; Linford, Matthew R.
2016-11-01
Peak fitting is an essential part of X-ray photoelectron spectroscopy (XPS) narrow scan analysis, and the Literature contains both good and bad examples of peak fitting. A common cause of poor peak fitting is the inclusion of too many fit parameters, often without a sound chemical and/or physical basis for them, and/or the failure to reasonably constrain them. Under these conditions, fit parameters are often correlated, and therefore lacking in statistical meaning. Here we introduce the uniqueness plot as a simple graphical tool for identifying bad peak fits in XPS, i.e., fit parameter correlation. These plots are widely used in spectroscopic ellipsometry. We illustrate uniqueness plots with two data sets: a C 1s narrow scan from ozone-treated carbon nanotube forests and an Si 2p narrow scan from an air-oxidized silicon wafer. For each fit, we consider different numbers of parameters and constraints on them. As expected, the uniqueness plots are parabolic when fewer fit parameters and/or more constraints are applied. However, they fan out and eventually become horizontal lines as more unconstrained parameters are included in the fits. Uniqueness plots are generated by plotting the chi squared (χ2) value for a fit vs. a systematically varied value of a parameter in the fit. The Abbe criterion is also considered as a figure of merit for uniqueness plots in the Supporting Information. We recommend that uniqueness plots be used by XPS practitioners for identifying inappropriate peak fits.
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Ren, Yu; Schlager, Hans; Martin, Damien
2014-01-01
A modified method for the quantitative determination of atmospheric perfluoroalkylcycloalkanes (PFCs) using thermal desorption coupled with gas chromatography and detection by negative ion chemical ionization-mass spectrometry was developed. Using an optimized analytical system, a commercially available Al 2 O 3 porous layer open tubular (PLOT) capillary column (30 m × 0.25 mm) deactivated with Na 2 SO 4 was used for separation of PFCs. Improvements in the separation of PFCs, the corresponding identification and the limit of detection of PFCs using this method and column are presented. The method was successfully applied to determine the atmospheric background concentrations of a range of PFCs from a number of samples collected at a rural site in Germany. The results of this study suggest that the method outlined using the Al 2 O 3 -PLOT-S capillary column has good sensitivity and selectivity, and that it can be deployed in a routine laboratory process for the analysis of PFCs in the future research work. In addition, the ability of this column to separate the isomers of one of the lower boiling PFCs (perfluorodimethylcyclobutane) and its ability to resolve perfluoroethylcyclohexane offer the opportunity for single-column analysis for multiple PFCs.
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Development of biochemical properties in anthropic soil (the study at Třinec–Jahodná plot
Directory of Open Access Journals (Sweden)
Karel Marosz
2013-01-01
Full Text Available The properties of the anthropic soils formed at the sludge bed “Třinec-Jahodná”. The sludge bed came from a long-time depositing of fly-ash and slag layers. Therefore, the anthropic soil properties obtained their features by both a character of layered substrate and a management of the local land reclamation. The paper presented deals with the rate of an intensity of biological and biochemical soil processes in charge of the fulfilment of plant nourishment demands, and the time viewpoints focusing on the local soil development. The set of enzymatic and biological measurements were chosen for treatments of soil bodies sampled throughout 2007–2008. The study plots inside the sludge bed and the control plot were sampled; the properties of particular horizons were studied. The results proved that the twenty-year-development of soil bodies made the proper conditions for plant nutrition. The positive statement, nevertheless, is directly linked to the presence of trees and shrubs. The vegetation seems to be one of the very crucial factors for a status of the site and maintenance of soil productivity: it affects temperature amplitudes, sensitivity to erosion, a redistribution of soil water, and a humic compounds accumulation. The statistical analyses showed significantly differing results on the study plots with a shorter development and a lower rate of vegetational cover.
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International Nuclear Information System (INIS)
Kaliakatsou, Evridiki; Bell, J. Nigel B.; Thirtle, Colin; Rose, Daniel; Power, Sally A.
2010-01-01
Numerous experiments have demonstrated reductions in the yields of cereal crops due to tropospheric O 3 , with losses of up to 25%. However, the only British econometric study on O 3 impacts on winter wheat yields, found that a 10% increase in AOT40 would decrease yields by only 0.23%. An attempt is made here to reconcile these observations by developing AOT40 maps for Great Britain and matching levels with a large number of standardised trial plot wheat yields from many sites over a 13-year period. Panel estimates (repeated measures on the same plots with time) show a 0.54% decrease in yields and it is hypothesised that plant breeders may have inadvertently selected for O 3 tolerance in wheat. Some support for this is provided by fumigations of cultivars of differing introduction dates. A case is made for the use of econometric as well as experimental studies in prediction of air pollution induced crop loss. - Econometric study of British winter wheat trial plot data suggests lower economic loss than predicted from experiments.
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Langan, Dean; Higgins, Julian P T; Gregory, Walter; Sutton, Alexander J
2012-05-01
We aim to illustrate the potential impact of a new study on a meta-analysis, which gives an indication of the robustness of the meta-analysis. A number of augmentations are proposed to one of the most widely used of graphical displays, the funnel plot. Namely, 1) statistical significance contours, which define regions of the funnel plot in which a new study would have to be located to change the statistical significance of the meta-analysis; and 2) heterogeneity contours, which show how a new study would affect the extent of heterogeneity in a given meta-analysis. Several other features are also described, and the use of multiple features simultaneously is considered. The statistical significance contours suggest that one additional study, no matter how large, may have a very limited impact on the statistical significance of a meta-analysis. The heterogeneity contours illustrate that one outlying study can increase the level of heterogeneity dramatically. The additional features of the funnel plot have applications including 1) informing sample size calculations for the design of future studies eligible for inclusion in the meta-analysis; and 2) informing the updating prioritization of a portfolio of meta-analyses such as those prepared by the Cochrane Collaboration. Copyright © 2012 Elsevier Inc. All rights reserved.
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Matthew F. Winn; Philip A. Araman
2012-01-01
The USDA Forest Service Forest Inventory and Analysis (FIA) program collects crown foliage transparency estimates for individual trees on Phase 3 (P3) inventory plots. The FIA crown foliage estimate is obtained from a pair of perpendicular side views of the tree. Researchers with the USDA Forest Service Southern Research Station have developed a computer program that...
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XNWLUP, Graphical user interface to plot WIMS-D library multigroup cross sections
International Nuclear Information System (INIS)
Ganesan, S.; Jagannathan, V.; Thiyagarajan, T.K.
2005-01-01
1 - Description of program or function: XnWlup is a computer program with user-friendly graphical interface to help the users of WIMS-D library to enable quick visualisation of the plots of the energy dependence of the multigroup cross sections of any nuclide of interest. This software enables the user to generate and view the histogram of 69 multi-group cross sections as a function of neutron energy under Microsoft Windows environment. This software is designed using Microsoft Visual C++ and Microsoft Foundation Classes Library. IAEA1395/05: New features of version 3.0: - Plotting absorption and fission cross sections of resonant nuclide after applying the self-shielding cross section. - Plotting the data of Resonant Integral table, as a function of dilution cross section for a selected temperature and for a given energy group. - Plotting the data of Resonant Integral table, as a function of temperature for a selected background dilution cross section and for a given energy group. - Clearing all the graphs except one graph from the display screen is easily done by using a tool bar button. - Displaying the coordinate of the cursor point with appropriate units. 2 - Methods: XnWlup helps to obtain histogram plots of the values of cross section data of an element/isotope available as 69-group WIMS-D library as a function of energy bins. The software XnWlup is developed with this graphical user interface in order to help those users who frequently refer to the WIMS-D library cross section data of neutron-nuclear reactions. The software also helps to produce handbook of WIMS-D cross sections
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karyoploteR: an R/Bioconductor package to plot customizable genomes displaying arbitrary data.
Gel, Bernat; Serra, Eduard
2017-10-01
Data visualization is a crucial tool for data exploration, analysis and interpretation. For the visualization of genomic data there lacks a tool to create customizable non-circular plots of whole genomes from any species. We have developed karyoploteR, an R/Bioconductor package to create linear chromosomal representations of any genome with genomic annotations and experimental data plotted along them. Plot creation process is inspired in R base graphics, with a main function creating karyoplots with no data and multiple additional functions, including custom functions written by the end-user, adding data and other graphical elements. This approach allows the creation of highly customizable plots from arbitrary data with complete freedom on data positioning and representation. karyoploteR is released under Artistic-2.0 License. Source code and documentation are freely available through Bioconductor (http://www.bioconductor.org/packages/karyoploteR) and at the examples and tutorial page at https://bernatgel.github.io/karyoploter_tutorial. bgel@igtp.cat. © The Author(s) 2017. Published by Oxford University Press.
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International Nuclear Information System (INIS)
Hersman, A.; Leege, P.F.A. de; Hoogenboom, J.E.
1992-04-01
The FORTRAN-77 program SCALPLO is being developed to make an easy and quick graphic survey of flux and/or power data calculated with SCALE modules or other core calculation or shielding codes. The basic plot functions it can perform are one- and two-dimensional plots of flux or power distributions and flux energy spectra. More specifically it can produce plots of the flux distribution in a one-dimensional geometry for one or more energy groups in one figure. It can also plot the flux distribution along a cut through a two- or three-dimensional geometry along one of the coordinate axes and it can plot a two-dimensional view of the flux distribution of a two-dimensional geometry or of a plane cut through a three-dimensional geometry. The same can be done for the power distribution in a system. Furthermore SCALPLO can plot the particle flux spectrum as a function of energy, either as group fluxes or as group fluxes per unit energy or per unit lethargy. (orig./HP)
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Measurement of the ω → π+π-π0 Dalitz plot distribution
Adlarson, P.; Augustyniak, W.; Bardan, W.; Bashkanov, M.; Bergmann, F. S.; Berłowski, M.; Bhatt, H.; Bondar, A.; Büscher, M.; Calén, H.; Ciepał, I.; Clement, H.; Czerwiński, E.; Demmich, K.; Engels, R.; Erven, A.; Erven, W.; Eyrich, W.; Fedorets, P.; Föhl, K.; Fransson, K.; Goldenbaum, F.; Goswami, A.; Grigoryev, K.; Gullström, C.-O.; Heijkenskjöld, L.; Hejny, V.; Hüsken, N.; Jarczyk, L.; Johansson, T.; Kamys, B.; Kemmerling, G.; Khan, F. A.; Khatri, G.; Khoukaz, A.; Khreptak, O.; Kirillov, D. A.; Kistryn, S.; Kleines, H.; Kłos, B.; Krzemień, W.; Kulessa, P.; Kupść, A.; Kuzmin, A.; Lalwani, K.; Lersch, D.; Lorentz, B.; Magiera, A.; Maier, R.; Marciniewski, P.; Mariański, B.; Morsch, H.-P.; Moskal, P.; Ohm, H.; Perez del Rio, E.; Piskunov, N. M.; Prasuhn, D.; Pszczel, D.; Pysz, K.; Pyszniak, A.; Ritman, J.; Roy, A.; Rudy, Z.; Rundel, O.; Sawant, S.; Schadmand, S.; Schätti-Ozerianska, I.; Sefzick, T.; Serdyuk, V.; Shwartz, B.; Sitterberg, K.; Skorodko, T.; Skurzok, M.; Smyrski, J.; Sopov, V.; Stassen, R.; Stepaniak, J.; Stephan, E.; Sterzenbach, G.; Stockhorst, H.; Ströher, H.; Szczurek, A.; Trzciński, A.; Varma, R.; Wolke, M.; Wrońska, A.; Wüstner, P.; Yamamoto, A.; Zabierowski, J.; Zieliński, M. J.; Złomańczuk, J.; Żuprański, P.; Żurek, M.; Kubis, B.; Leupold, S.
2017-07-01
Using the production reactions pd →3He ω and pp → ppω, the Dalitz plot distribution for the ω →π+π-π0 decay is studied with the WASA detector at COSY, based on a combined data sample of (4.408 ± 0.042) ×104 events. The Dalitz plot density is parametrised by a product of the P-wave phase space and a polynomial expansion in the normalised polar Dalitz plot variables Z and ϕ. For the first time, a deviation from pure P-wave phase space is observed with a significance of 4.1σ. The deviation is parametrised by a linear term 1 + 2 αZ, with α determined to be + 0.147 ± 0.036, consistent with the expectations of ρ-meson-type final-state interactions of the P-wave pion pairs.
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Amplitude analysis of the D+ -> K-S(0)pi + (0)(pi) Dalitz plot
Ablikim, M.; Achasov, M. N.; Ai, X. C.; Albayrak, O.; Albrecht, M.; Ambrose, D. J.; An, F. F.; An, Q.; Bai, J. Z.; Ferroli, R. Baldini; Ban, Y.; Bennett, J. V.; Bertani, M.; Bian, J. M.; Boger, E.; Bondarenko, O.; Boyko, I.; Braun, S.; Briere, R. A.; Cai, H.; Cai, X.; Cakir, O.; Calcaterra, A.; Cao, G. F.; Cetin, S. A.; Chang, J. F.; Chelkov, G.; Chen, G.; Chen, H. S.; Chen, J. C.; Chen, M. L.; Chen, S. J.; Chen, X.; Chen, X. R.; Chen, Y. B.; Cheng, H. P.; Chu, X. K.; Chu, Y. P.; Cronin-Hennessy, D.; Dai, H. L.; Dai, J. P.; Dedovich, D.; Deng, Z. Y.; Denig, A.; Denysenko, I.; Kalantar-Nayestanaki, N.; Kavatsyuk, M.; Loehner, H.; Messchendorp, J. G.; Moeini, H.
2014-01-01
We perform an analysis of the D+ -> K-S(0)pi + (0)(pi) Dalitz plot using a data set of 2.92 fb(-1) of e(+) e(-) collisions at the (3770) mass accumulated by the BESIII experiment, in which 166694 candidate events are selected with a background of 15.1%. The Dalitz plot is found to be well
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The Brazilian national system of forest permanent plots
Yeda Maria Malheiros de Oliveira; Maria Augusta Doetzer Rosot; Patricia Povoa de Mottos; Joberto Veloso de Freitas; Guilherme Luis Augusto Gomide; < i> et al< /i>
2009-01-01
The Brazilian National System of Forest Permanent Plots (SisPP) is a governmental initiative designed and being implemented in partnership by the Ministry of Environment (MMA), represented by the National Forest Programme (PNF) and the Brazilian Forest Service (SFB) and the Embrapa Forestry (a research center of the Brazilian Agricultural Research Corporation - Embrapa...
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Energy Technology Data Exchange (ETDEWEB)
Burris, R.D.; Gray, W.H.
1978-01-01
A command which causes the translation of any supported graphics file format to a format acceptable to any supported device was implemented on two linked DECsystem-10s. The processing of the command is divided into parsing and translating phases. In the parsing phase, information is extracted from the command and augmented by default data. The results of this phase are saved on disk, and the appropriate translating routine is invoked. Twenty-eight translating programs were implemented in this system. They support four different graphics file formats, including the DISSPLA and Calcomp formats, and seven different types of plotters, including Tektronix, Calcomp, and Versatec devices. Some of the plotters are devices linked to the DECsystem-10s, and some are driven by IBM System/360 computers linked via a communications network to the DECsystem-10s. The user of this facility can use any of the supported packages to create a file of graphics data, preview the file on an on-line scope, and, when satisfied, cause the same data to be plotted on a hard-copy device. All of the actions utilize a single simple command format. 2 figures.
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Plot-slope soil erosion using 7Be measurement and rill fractal dimension
International Nuclear Information System (INIS)
Zhang Fengbao; Yang Mingyi
2010-01-01
In this study, we intended to use 7 Be measurement and fractal theory to quantify soil erosion process on slope. The results showed that contribution rate of inter rill erosion was more than that of rill erosion during early stage of rainfall. When it rained, contribution rate of rill erosion began to be higher than inter rill erosion and become the main part of erosion during medium stage of rainfall. The trend of contribution rate of inter rill erosion was growing and the rill erosion was lowering during late stage of rainfall. Rill fractal dimension on the plot slope was almost growing larger during rainfall,growing quickly during early stage of rainfall and slowly during the late stage. Correlations was positive between rill fractal dimension and total erosion amount, also positive between rill fractal dimension and rill erosion. The correlations was positive between rill fractal dimension variation and total erosion amount, also was positive between rill fractal dimension variation and rill erosion amount. The best correlation was observed between rill fractal dimension and rill erosion amount. These results indicated that the rill fractal dimension on the plot slope could represent the development process of rill,the complex degree of rill and the variation of soil erosion intensity on the entire slope. (authors)
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The isometric log-ratio (ilr)-ion plot: A proposed alternative to the Piper diagram
Shelton, Jenna L.; Engle, Mark A.; Buccianti, Antonella; Blondes, Madalyn S.
2018-01-01
The Piper diagram has been a staple for the analysis of water chemistry data since its introduction in 1944. It was conceived to be a method for water classification, determination of potential water mixing between end-members, and to aid in the identification of chemical reactions controlling a sample set. This study uses the information gleaned over the years since the release of the Piper diagram and proposes an alternative to it, capturing the strengths of the original diagram while adding new ideas to increase its robustness. The new method uses compositional data analysis to create 4 isometric log-ratio coordinates for the 6 major chemical species analyzed in the Piper diagram and transforms the data to a 4-field bi-plot, the ilr-ion plot. This ilr-ion plot conveys all of the information in the Piper diagram (water mixing, water types, and chemical reactions) while also visualizing additional data, the ability to examine Ca2+/Mg2+ versus Cl-/SO42−. The Piper and the ilr-ion plot were also compared using multiple synthetic and real datasets in order to illustrate the caveats and the advantages of using either diagram to analyze water chemistry data. Although there are challenges with using the ilr-ion plot (e.g., missing or zero values zeros in the dataset must be imputed by positive real numbers), it appears that the use of compositional data analysis coupled with the ilr-ion plot provides a more in-depth and complete analysis of water quality data compared to the original Piper diagram.
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LAND SURVEY AND CADASTRAL MEASUREMENT MADE FOR AN PLOT PLAN COMPLEX
Directory of Open Access Journals (Sweden)
Madalina Marian
2012-12-01
Full Text Available According to Law no. 7/1996 of Cadastre and Real Estate Publicity, plan plot is a strip ground graphical representation containing strip ground limits and details of all the buildings that define stable land and cadastral division strip ground is technical administrative unit defined by fixed details, identifiable field that does not change over time, such as roads, water, dams, etc. This paper presents how to make a plot plan strip ground covering a 4 forest Ups (production unit and 108UAs (limits of forestry, land use is forest, pasture and village road. High surface is located extraurban limits Arefu in Arges County and has 28185443mp, that’ s equivalent to 2818,54ha. Forest cadastre specific limits are drawn in the work arrangement of forests and are represented by plot limit and the subparcels limit. This paper aims to presenting the main components of a modern surveying device used in our country, to carry out surveying works for forest cadastre. To use GPS technology (Global Position System in combination with the total station.
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Graffelman, Jan; van Eeuwijk, Fred
2005-12-01
The scatter plot is a well known and easily applicable graphical tool to explore relationships between two quantitative variables. For the exploration of relations between multiple variables, generalisations of the scatter plot are useful. We present an overview of multivariate scatter plots focussing on the following situations. Firstly, we look at a scatter plot for portraying relations between quantitative variables within one data matrix. Secondly, we discuss a similar plot for the case of qualitative variables. Thirdly, we describe scatter plots for the relationships between two sets of variables where we focus on correlations. Finally, we treat plots of the relationships between multiple response and predictor variables, focussing on the matrix of regression coefficients. We will present both known and new results, where an important original contribution concerns a procedure for the inclusion of scales for the variables in multivariate scatter plots. We provide software for drawing such scales. We illustrate the construction and interpretation of the plots by means of examples on data collected in a genomic research program on taste in tomato.
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Dissociations of spatial congruence effects across response measures: an examination of delta plots.
Miller, Jeff; Roüast, Nora M
2016-09-01
Spatial congruence ("Simon") effects on reaction time (RT) and response force (RF) were studied in two experiments requiring speeded choice responses to the color of a stimulus located irrelevantly to the left or right of fixation. In Experiment 1 with unimanual responses, both RT and incorrect-hand RF were sensitive to spatial congruence, and both showed larger Simon effects following a congruent trial than following an incongruent one. RT and incorrect-hand RF were dissociated in distributional (i.e., delta plot) analyses, however. As in previous studies, the Simon effect on RT was largest for the fastest responses and diminished as RT increased (i.e., decreasing delta plot). In contrast, Simon effects on RF did not decrease for slower responses; if anything, they increased slightly. In Experiment 2 participants made bimanual responses, allowing measurement of the spatial congruence effect for each trial. Responses were both faster and more forceful with the spatially congruent hand than with the spatially incongruent one, but neither of these effects decreased for slower responses. Overall, the results demonstrate that at least some motor-level effects of irrelevant spatial location persist for slower responses.
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First principle DFT study of electric field effects on the characteristics of bilayer graphene
Energy Technology Data Exchange (ETDEWEB)
Sabzyan, Hassan; Sadeghpour, Narges [Isfahan Univ. (Iran, Islamic Republic of). Dept. of Chemistry
2017-04-01
First principle density functional theory methods, local density and Perdew-Burke-Ernzerhof generalized gradient approximations with Goedecker pseudopotential (LDA-G and PBE-G), are used to study the electric field effects on the binding energy and atomic charges of bilayer graphene (BLG) at the Γ point of the Brillouin zone based on two types of unit cells (α and β) containing n{sub C}=8-32 carbon atoms. Results show that application of electric fields of 4-24 V/nm strengths reduces the binding energies and induces charge transfer between the two layers. The transferred charge increases almost linearly with the strength of the electric field for all sizes of the two types of unit cells. Furthermore, the charge transfer calculated with the α-type unit cells is more sensitive to the electric field strength. The calculated field-dependent contour plots of the differential charge densities of the two layers show details of charge density redistribution under the influence of the electric field.
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The interpretation of quartz optically stimulated luminescence equivalent dose versus time plots
International Nuclear Information System (INIS)
Bailey, R.M.
2000-01-01
Numerical modelling has shown that the form of the quartz OSL shine plateau (hereafter 'D e (t)-plot') is influenced by the effects of phototransferred TL in the ∼110 deg. C region. It is suggested also that the presence of multiple OSL components (as described by Partial bleaching and the decay form characteristics of quartz OSL. Radiat. Meas., 27, 123-136. The form of the optically stimulated luminescence signal of quartz: implications of dating. Unpublished PhD thesis, University of London) affects the form of the D e (t)-plot. Laboratory measurements of a fully reset and artificially dosed sample yielded non-flat D e (t)-plots, the deviation being greater for the larger of the two simulated palaeodoses, in accordance with theoretical predictions. It is suggested that the so-called 'shine plateau' test is of limited use in assessing the bleaching history of quartz sediments
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Karpefors, Martin; Weatherall, James
2018-03-21
In contrast to efficacy, safety hypotheses of clinical trials are not always pre-specified, and therefore, the safety interpretation work of a trial tends to be more exploratory, often reactive, and the analysis more statistically and graphically challenging. We introduce a new means of visualizing the adverse event data across an entire clinical trial. The approach overcomes some of the current limitations of adverse event analysis and streamlines the way safety data can be explored, interpreted and analyzed. Using a phase II study, we describe and exemplify how the tendril plot effectively summarizes the time-resolved safety profile of two treatment arms in a single plot and how that can provide scientists with a trial safety overview that can support medical decision making. To our knowledge, the tendril plot is the only way to graphically show important treatment differences with preserved temporal information, across an entire clinical trial, in a single view.
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Harrison, Sean; Jones, Hayley E; Martin, Richard M; Lewis, Sarah J; Higgins, Julian P T
2017-09-01
Meta-analyses combine the results of multiple studies of a common question. Approaches based on effect size estimates from each study are generally regarded as the most informative. However, these methods can only be used if comparable effect sizes can be computed from each study, and this may not be the case due to variation in how the studies were done or limitations in how their results were reported. Other methods, such as vote counting, are then used to summarize the results of these studies, but most of these methods are limited in that they do not provide any indication of the magnitude of effect. We propose a novel plot, the albatross plot, which requires only a 1-sided P value and a total sample size from each study (or equivalently a 2-sided P value, direction of effect and total sample size). The plot allows an approximate examination of underlying effect sizes and the potential to identify sources of heterogeneity across studies. This is achieved by drawing contours showing the range of effect sizes that might lead to each P value for given sample sizes, under simple study designs. We provide examples of albatross plots using data from previous meta-analyses, allowing for comparison of results, and an example from when a meta-analysis was not possible. Copyright © 2017 The Authors. Research Synthesis Methods Published by John Wiley & Sons Ltd.
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Optimal plot size in the evaluation of papaya scions: proposal and comparison of methods
Directory of Open Access Journals (Sweden)
Humberto Felipe Celanti
Full Text Available ABSTRACT Evaluating the quality of scions is extremely important and it can be done by characteristics of shoots and roots. This experiment evaluated height of the aerial part, stem diameter, number of leaves, petiole length and length of roots of papaya seedlings. Analyses were performed from a blank trial with 240 seedlings of "Golden Pecíolo Curto". The determination of the optimum plot size was done by applying the methods of maximum curvature, maximum curvature of coefficient of variation and a new proposed method, which incorporates the bootstrap resampling simulation to the maximum curvature method. According to the results obtained, five is the optimal number of seedlings of papaya "Golden Pecíolo Curto" per plot. The proposed method of bootstrap simulation with replacement provides optimal plot sizes equal or higher than the maximum curvature method and provides same plot size than maximum curvature method of the coefficient of variation.
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Elze, Markus C; Gimeno, Hortensia; Tustin, Kylee; Baker, Lesley; Lumsden, Daniel E; Hutton, Jane L; Lin, Jean-Pierre S-M
2016-02-01
Hyperkinetic movement disorders (HMDs) can be assessed using impairment-based scales or functional classifications. The Burke-Fahn-Marsden Dystonia Rating Scale-movement (BFM-M) evaluates dystonia impairment, but may not reflect functional ability. The Gross Motor Function Classification System (GMFCS), Manual Ability Classification System (MACS), and Communication Function Classification System (CFCS) are widely used in the literature on cerebral palsy to classify functional ability, but not in childhood movement disorders. We explore the concordance of these three functional scales in a large sample of paediatric HMDs and the impact of dystonia severity on these scales. Children with HMDs (n=161; median age 10y 3mo, range 2y 6mo-21y) were assessed using the BFM-M, GMFCS, MACS, and CFCS from 2007 to 2013. This cross-sectional study contrasts the information provided by these scales. All four scales were strongly associated (all Spearman's rank correlation coefficient rs >0.72, pdisorders including cerebral palsy can be effectively evaluated using these scales. © 2015 Mac Keith Press.
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Liu, Dan; Hu, Kai; Nordbeck, Peter; Ertl, Georg; Störk, Stefan; Weidemann, Frank
2016-05-10
Despite substantial advances in the imaging techniques and pathophysiological understanding over the last decades, identification of the underlying causes of left ventricular hypertrophy by means of echocardiographic examination remains a challenge in current clinical practice. The longitudinal strain bull's eye plot derived from 2D speckle tracking imaging offers an intuitive visual overview of the global and regional left ventricular myocardial function in a single diagram. The bull's eye mapping is clinically feasible and the plot patterns could provide clues to the etiology of cardiomyopathies. The present review summarizes the longitudinal strain, bull's eye plot features in patients with various cardiomyopathies and concentric left ventricular hypertrophy and the bull's eye plot features might serve as one of the cardiac workup steps on evaluating patients with left ventricular hypertrophy.
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Insights into gait disorders: walking variability using phase plot analysis, Huntington's disease.
Collett, Johnny; Esser, Patrick; Khalil, Hanan; Busse, Monica; Quinn, Lori; DeBono, Katy; Rosser, Anne; Nemeth, Andrea H; Dawes, Helen
2014-09-01
Huntington's disease (HD) is a progressive inherited neurodegenerative disorder. Identifying sensitive methodologies to quantitatively measure early motor changes have been difficult to develop. This exploratory observational study investigated gait variability and symmetry in HD using phase plot analysis. We measured the walking of 22 controls and 35 HD gene carriers (7 premanifest (PreHD)), 16 early/mid (HD1) and 12 late stage (HD2) in Oxford and Cardiff, UK. The unified Huntington's disease rating scale-total motor scores (UHDRS-TMS) and disease burden scores (DBS) were used to quantify disease severity. Data was collected during a clinical walk test (8.8 or 10 m) using an inertial measurement unit attached to the trunk. The 6 middle strides were used to calculate gait variability determined by spatiotemporal parameters (co-efficient of variation (CoV)) and phase plot analysis. Phase plots considered the variability in consecutive wave forms from vertical movement and were quantified by SDA (spatiotemporal variability), SDB (temporal variability), ratio ∀ (ratio SDA:SDB) and Δangleβ (symmetry). Step time CoV was greater in manifest HD (p0.05). Phase plot analysis identified differences between manifest HD and controls for SDB, Ratio ∀ and Δangle (all pplot analysis may be a sensitive method of detecting gait changes in HD and can be performed quickly during clinical walking tests. Copyright © 2014 Elsevier B.V. All rights reserved.
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Relative Efficiency of Split-plot Design (SPD) to Randomized ...
African Journals Online (AJOL)
PROF. O. E. OSUAGWU
2013-06-01
Jun 1, 2013 ... West African Journal of Industrial and Academic Research Vol.7 No. 1 June .... effects. They concluded that the proposed efficiency measures provide feasible solutions for .... Fm = d.f for the whole-plot treatment; Fb= d.f for the ...
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In vitro inactivation of hepatic microsomal phospholipase A2 by the marine natural product manoalide
International Nuclear Information System (INIS)
Master, M.M.; Jacobs, R.S.
1986-01-01
The effects of manoalide (MLD) and several analogs (isolated from the sponge Luffariella variabilis) on mouse hepatic microsomal phospholipase A 2 (PLA 2 ) activity was investigated. Microsomal PLA 2 , a membrane bound, Ca ++ dependent enzyme with an alkaline pH optimum, functions in intracellular phospholipid turnover. In vitro PLA 2 activity was assayed by preincubating MLD or analogs (2.5-100μM) with microsomes for 60 min. at 37 0 C, combining this mixture with 14 C-phosphatidylcholine and CaCl 2 , and incubating at 37 0 C for 40 minutes. Enzyme activity was quantitated by measurement of the extracted 14 C-arachidonic acid product. MLD inhibited PLA 2 in a dose-dependent manner, with an IC 50 = 94μM. Lineweaver-Burk analysis suggests that MLD inhibits PLA 2 noncompetitively. One of the analogs, producing a comparable dose-response curve to MLD, was found to be more potent (IC 50 = 33μM). Another analog facilitated PLA 2 activity (15%) at 25μM, followed by inactivation at higher doses (IC 50 > 100 μM). Facilitation of PLA 2 activity was seen with concentrations as low as 2.5μM of a third analog, and significant inactivation of PLA 2 was evident. These results indicate that MLD is not as potent against microsomal PLA 2 as has been shown with purified bee venom and cobra venom PLA 2 's
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Laccase immobilized on methylene blue modified mesoporous silica MCM-41/PVA
International Nuclear Information System (INIS)
Xu Xinhua; Lu Ping; Zhou Yumei; Zhao Zhenzhen; Guo Meiqing
2009-01-01
The mesoporous silica sieve MCM-41 containing methylene blue (MB) provides a suitable immobilization of biomolecule matrix due to its uniform pore structure, high surface areas, good biocompatibility and nice conductivity. Based on this, a facilely fabricated amperometric biosensor by entrapping laccase into the MB modified MCM-41/PVA composite film has been developed. Laccase from Trametes versicolor is assembled on a composite film of MCM-41 containing MB/PVA modified Au electrode and the electrode is characterized with respect to transmission electron microscopy (TEM) and scanning electron microscopic (SEM), Cyclic voltammetry (CV), response time, detection limit, linear range and activity of laccase. The laccase modified electrode remains good redox behavior in pH 4.95 acetate buffer solution, at room temperature in present of 0.1 mM catechol. The response time (t 90% ) of the modified electrode is less than 4 s for catechol. The detection limit is 0.331 μM and the linear detect range is about from 4.0 μM to 87.98 μM for catechol with a correlation coefficient of 0.99913(S/N = 3). The apparent Michaelis-Menten (K M app ) is estimated using the Lineweaver-Burk equation and the K M app value is about 0.256 mM. This work demonstrated that the mesoporous silica MCM-41 containing MB provides a novel support for laccase immobilization and the construction of biosensors with a faster response and better bioactivity.
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Governing in the space of the "Seam" : Airport security after the liquid bomb plot
Hoijtink, M.
This paper provides a detailed study of the liquid bomb plot from 2006, focusing on the ways in which the plot was constituted as “an event unlike others” (Adey, Anderson, and Lobo Guerrero 2011, 340). Engaging with a critical body of scholarship that examines how events are assembled and governed
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The pros and cons of funnel plots as an aid to risk communication and patient decision making.
Rakow, Tim; Wright, Rebecca J; Spiegelhalter, David J; Bull, Catherine
2015-05-01
Funnel plots, which simultaneously display a sample statistic and the corresponding sample size for multiple cases, have a range of applications. In medicine, they are used to display treatment outcome rates and caseload volume by institution, which can inform strategic decisions about health care delivery. We investigated lay people's understanding of such plots and explored their suitability as an aid to individual treatment decisions. In two studies, 172 participants answered objective questions about funnel plots representing the surgical outcomes (survival or mortality rates) of institutions varying in caseload, and indicated their preferred institutions. Accuracy for extracting objective information was high, unless question phrasing was inconsistent with the plot's survival/mortality framing, or participants had low numeracy levels. Participants integrated caseload-volume and outcome-rate data when forming preferences, but were influenced by reference lines on the plot to make inappropriate discriminations between institutions with similar outcome rates. With careful choice of accompanying language, funnel plots can be readily understood and are therefore a useful tool for communicating risk. However, they are less effective as a decision aid for individual patient's treatment decisions, and we recommend refinements to the standard presentation of the plots if they are to be used for that purpose. © 2014 The British Psychological Society.
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Directory of Open Access Journals (Sweden)
R. Biggs
2003-12-01
Full Text Available The experimental burn plot (EBP trial initiated in 1954 is one of few ongoing long-termfire ecology research projects in Africa. The trial aims to assess the impacts of differentfire regimes in the Kruger National Park. Recent studies on the EBPs have raised questions as to the experimental design of the trial, and the appropriate model specificationwhen analysing data. Archival documentation reveals that the original design was modified on several occasions, related to changes in the park's fire policy. These modifications include the addition of extra plots, subdivision of plots and changes in treatmentsover time, and have resulted in a design which is only partially randomised. The representativity of the trial plots has been questioned on account of their relatively small size,the concentration of herbivores on especially the frequently burnt plots, and soil variation between plots. It is suggested that these factors be included as covariates inexplanatory models or that certain plots be excluded from data analysis based on resultsof independent studies of these factors. Suggestions are provided for the specificationof the experimental design when analysing data using Analysis of Variance. It is concluded that there is no practical alternative to treating the trial as a fully randomisedcomplete block design.
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Chuang, Shin-Shin; Wu, Kung-Tai; Lin, Chen-Yang; Lee, Steven; Chen, Gau-Yang; Kuo, Cheng-Deng
2014-08-01
The Poincaré plot of RR intervals (RRI) is obtained by plotting RRIn+1 against RRIn. The Pearson correlation coefficient (ρRRI), slope (SRRI), Y-intercept (YRRI), standard deviation of instantaneous beat-to-beat RRI variability (SD1RR), and standard deviation of continuous long-term RRI variability (SD2RR) can be defined to characterize the plot. Similarly, the Poincaré plot of autocorrelation function (ACF) of RRI can be obtained by plotting ACFk+1 against ACFk. The corresponding Pearson correlation coefficient (ρACF), slope (SACF), Y-intercept (YACF), SD1ACF, and SD2ACF can be defined similarly to characterize the plot. By comparing the indices of Poincaré plots of RRI and ACF between patients with acute myocardial infarction (AMI) and patients with patent coronary artery (PCA), we found that the ρACF and SACF were significantly larger, whereas the RMSSDACF/SDACF and SD1ACF/SD2ACF were significantly smaller in AMI patients. The ρACF and SACF correlated significantly and negatively with normalized high-frequency power (nHFP), and significantly and positively with normalized very low-frequency power (nVLFP) of heart rate variability in both groups of patients. On the contrary, the RMSSDACF/SDACF and SD1ACF/SD2ACF correlated significantly and positively with nHFP, and significantly and negatively with nVLFP and low-/high-frequency power ratio (LHR) in both groups of patients. We concluded that the ρACF, SACF, RMSSDACF/SDACF, and SD1ACF/SD2ACF, among many other indices of ACF Poincaré plot, can be used to differentiate between patients with AMI and patients with PCA, and that the increase in ρACF and SACF and the decrease in RMSSDACF/SDACF and SD1ACF/SD2ACF suggest an increased sympathetic and decreased vagal modulations in both groups of patients.
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Split-plot Experiments with Unusual Numbers of Subplot Runs
DEFF Research Database (Denmark)
Kulahci, Murat
2007-01-01
In many experimental situations, it may not be feasible or even possible to run experiments in a completely randomized fashion as usually recommended. Under these circumstances, split-plot experiments in which certain factors are changed less frequently than the others are often used. Most...
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Designing fractional factorial split-plot experiments using integer programming
DEFF Research Database (Denmark)
Capehart, Shay R.; Keha, Ahmet; Kulahci, Murat
2011-01-01
factorial (FF) design, with the restricted randomisation structure to account for the whole plots and subplots. We discuss the formulation of FFSP designs using integer programming (IP) to achieve various design criteria. We specifically look at the maximum number of clear two-factor interactions...
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Soil Characteristics, Microbial Compostion of Plot, Leaf Count and ...
African Journals Online (AJOL)
Soil Characteristics, Microbial Compostion of Plot, Leaf Count and Sprout Studies of Cocoyam ( Colocasia [Schott] and Xanthosoma [Schott], Araceae) Collected in Edo State, ... Science, Technology and Arts Research Journal ... Government Areas (LGA) in Edo state and describe them based on leaf count and sprout
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Wood, Sam W.; Prior, Lynda D.; Stephens, Helen C.; Bowman, David M. J. S.
2015-01-01
Tracking the response of forest ecosystems to climate change demands large (≥1 ha) monitoring plots that are repeatedly measured over long time frames and arranged across macro-ecological gradients. Continental scale networks of permanent forest plots have identified links between climate and carbon fluxes by monitoring trends in tree growth, mortality and recruitment. The relationship between tree growth and climate in Australia has been recently articulated through analysis of data from smaller forest plots, but conclusions were limited by (a) absence of data on recruitment and mortality, (b) exclusion of non-eucalypt species, and (c) lack of knowledge of stand age or disturbance histories. To remedy these gaps we established the Ausplots Forest Monitoring Network: a continental scale network of 48 1 ha permanent plots in highly productive tall eucalypt forests in the mature growth stage. These plots are distributed across cool temperate, Mediterranean, subtropical and tropical climates (mean annual precipitation 850 to 1900 mm per year; mean annual temperature 6 to 21°C). Aboveground carbon stocks (AGC) in these forests are dominated by eucalypts (90% of AGC) whilst non-eucalypts in the understorey dominated species diversity and tree abundance (84% of species; 60% of stems). Aboveground carbon stocks were negatively related to mean annual temperature, with forests at the warm end of the temperature range storing approximately half the amount of carbon as forests at the cool end of the temperature range. This may reflect thermal constraints on tree growth detected through other plot networks and physiological studies. Through common protocols and careful sampling design, the Ausplots Forest Monitoring Network will facilitate the integration of tall eucalypt forests into established global forest monitoring initiatives. In the context of projections of rapidly warming and drying climates in Australia, this plot network will enable detection of links between
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Directory of Open Access Journals (Sweden)
David Lee Erickson
2014-11-01
Full Text Available Forest dynamics plots, which now span longitudes, latitudes, and habitat types across the globe, offer unparalleled insights into the ecological and evolutionary processes that determine how species are assembled into communities. Understanding phylogenetic relationships among species in a community has become an important component of assessing assembly processes. However, the application of evolutionary information to questions in community ecology has been limited in large part by the lack of accurate estimates of phylogenetic relationships among individual species found within communities, and is particularly limiting in comparisons between communities. Therefore, streamlining and maximizing the information content of these community phylogenies is a priority. To test the viability and advantage of a multi-community phylogeny, we constructed a multi-plot mega-phylogeny of 1,347 species of trees across 15 forest dynamics plots in the ForestGEO network using DNA barcode sequence data (rbcL, matK and psbA-trnH and compared community phylogenies for each individual plot with respect to support for topology and branch lengths, which affect evolutionary inference of community processes. The levels of taxonomic differentiation across the phylogeny were examined by quantifying the frequency of resolved nodes throughout. In addition, three phylogenetic distance metrics that are commonly used to infer assembly processes were estimated for each plot (Phylogenetic Distance [PD], Mean Phylogenetic Distance [MPD], and Mean Nearest Taxon Distance [MNTD]. Lastly, we examine the partitioning of phylogenetic diversity among community plots through quantification of inter-community MPD and MNTD. Overall, evolutionary relationships were highly resolved across the DNA barcode-based mega-phylogeny, and phylogenetic resolution for each community plot was improved when estimated within the context of the mega-phylogeny. Likewise, when compared with phylogenies for
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Ito, Hiroshi; Yokoi, Takashi; Ikoma, Yoko; Shidahara, Miho; Seki, Chie; Naganawa, Mika; Takahashi, Hidehiko; Takano, Harumasa; Kimura, Yuichi; Ichise, Masanori; Suhara, Tetsuya
2010-01-01
In positron emission tomography (PET) studies with radioligands for neuroreceptors, tracer kinetics have been described by the standard two-tissue compartment model that includes the compartments of nondisplaceable binding and specific binding to receptors. In the present study, we have developed a new graphic plot analysis to determine the total distribution volume (V(T)) and nondisplaceable distribution volume (V(ND)) independently, and therefore the binding potential (BP(ND)). In this plot, Y(t) is the ratio of brain tissue activity to time-integrated arterial input function, and X(t) is the ratio of time-integrated brain tissue activity to time-integrated arterial input function. The x-intercept of linear regression of the plots for early phase represents V(ND), and the x-intercept of linear regression of the plots for delayed phase after the equilibrium time represents V(T). BP(ND) can be calculated by BP(ND)=V(T)/V(ND)-1. Dynamic PET scanning with measurement of arterial input function was performed on six healthy men after intravenous rapid bolus injection of [(11)C]FLB457. The plot yielded a curve in regions with specific binding while it yielded a straight line through all plot data in regions with no specific binding. V(ND), V(T), and BP(ND) values calculated by the present method were in good agreement with those by conventional non-linear least-squares fitting procedure. This method can be used to distinguish graphically whether the radioligand binding includes specific binding or not.
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Analysis of a Split-Plot Experimental Design Applied to a Low-Speed Wind Tunnel Investigation
Erickson, Gary E.
2013-01-01
A procedure to analyze a split-plot experimental design featuring two input factors, two levels of randomization, and two error structures in a low-speed wind tunnel investigation of a small-scale model of a fighter airplane configuration is described in this report. Standard commercially-available statistical software was used to analyze the test results obtained in a randomization-restricted environment often encountered in wind tunnel testing. The input factors were differential horizontal stabilizer incidence and the angle of attack. The response variables were the aerodynamic coefficients of lift, drag, and pitching moment. Using split-plot terminology, the whole plot, or difficult-to-change, factor was the differential horizontal stabilizer incidence, and the subplot, or easy-to-change, factor was the angle of attack. The whole plot and subplot factors were both tested at three levels. Degrees of freedom for the whole plot error were provided by replication in the form of three blocks, or replicates, which were intended to simulate three consecutive days of wind tunnel facility operation. The analysis was conducted in three stages, which yielded the estimated mean squares, multiple regression function coefficients, and corresponding tests of significance for all individual terms at the whole plot and subplot levels for the three aerodynamic response variables. The estimated regression functions included main effects and two-factor interaction for the lift coefficient, main effects, two-factor interaction, and quadratic effects for the drag coefficient, and only main effects for the pitching moment coefficient.
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Analysis and Visualization of ChIP-Seq and RNA-Seq Sequence Alignments Using ngs.plot.
Loh, Yong-Hwee Eddie; Shen, Li
2016-01-01
The continual maturation and increasing applications of next-generation sequencing technology in scientific research have yielded ever-increasing amounts of data that need to be effectively and efficiently analyzed and innovatively mined for new biological insights. We have developed ngs.plot-a quick and easy-to-use bioinformatics tool that performs visualizations of the spatial relationships between sequencing alignment enrichment and specific genomic features or regions. More importantly, ngs.plot is customizable beyond the use of standard genomic feature databases to allow the analysis and visualization of user-specified regions of interest generated by the user's own hypotheses. In this protocol, we demonstrate and explain the use of ngs.plot using command line executions, as well as a web-based workflow on the Galaxy framework. We replicate the underlying commands used in the analysis of a true biological dataset that we had reported and published earlier and demonstrate how ngs.plot can easily generate publication-ready figures. With ngs.plot, users would be able to efficiently and innovatively mine their own datasets without having to be involved in the technical aspects of sequence coverage calculations and genomic databases.
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EVALPLOT2007, ENDF Plots Cross Section, Angular Distribution and Energy Distribution
International Nuclear Information System (INIS)
2007-01-01
1 - Description of program or function: EVALPLOT is designed to plot evaluated cross sections in the ENDF/B format. The program plots cross sections, angular distributions, energy distributions and other parameters. IAEA1322/16: This version include the updates up to January 30, 2007. Changes in ENDF/B-VII Format and procedures, as well as the evaluations themselves, make it impossible for versions of the ENDF/B pre-processing codes earlier than PREPRO 2007 (2007 Version) to accurately process current ENDF/B-VII evaluations. The present code can handle all existing ENDF/B-VI evaluations through release 8, which will be the last release of ENDF/B-VI. Modifications from previous versions: Evalplot Vers. 2007-1 (Jan. 2007): - checked against all ENDF/B-VII; - increased page size from 600,000 to 2,400,000; - increased the number of energies vs. legendre coefficients from 20,000 to 80,000 (must be 1/30 page size); - added (n,remainder) to first plot. 2 - Method of solution: In the case of processing neutron and photon cross sections (MF=3 or 23) and parameters (MF=1 or 27), all data in a file (MF) is read, grouped together by type, and plotted. All reactions of a data type appear on the same plot. The data types for MF=1 and 3 (neutrons) are: (1) total, elastic, capture, fission and total inelastic; (2) (n,2n), (n,3n) and (n,n' charged particle); (3) (n,charged particle); (4) particle production (proton, deuteron, etc.) and damage; (5) total, first, second, etc. chance fission; (6) total inelastic, inelastic discrete levels and continuum; (7) (n,p) total and levels (only if levels are given); (8) (n,d) total and levels (only if levels are given); (9) (n,t) total and levels (only if levels are given); (10) (n, 3 He) total and levels (only if levels are given); (11) (n, 4 He) total and levels (only if levels are given); (12) parameters mu-bar, xi and gamma; (13) nu-bar - total, prompt an delayed. The data types for MF=23 and 27 (photons) are: (14) total, coherent
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Toyama, Hironori; Kajisa, Tsuyoshi; Tagane, Shuichiro; Mase, Keiko; Chhang, Phourin; Samreth, Vanna; Ma, Vuthy; Sokh, Heng; Ichihashi, Ryuji; Onoda, Yusuke; Mizoue, Nobuya; Yahara, Tetsukazu
2015-01-01
Ecological communities including tropical rainforest are rapidly changing under various disturbances caused by increasing human activities. Recently in Cambodia, illegal logging and clear-felling for agriculture have been increasing. Here, we study the effects of logging, mortality and recruitment of plot trees on phylogenetic community structure in 32 plots in Kampong Thom, Cambodia. Each plot was 0.25 ha; 28 plots were established in primary evergreen forests and four were established in se...
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Research to measure soil erosion rates in the United States from natural rainfall runoff plots began in the early 1900’s. In Brazil, the first experimental study at the plot-scale was conducted in the 1940’s; however, the monitoring process and the creation of new experimental field plots have not c...
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International Nuclear Information System (INIS)
Uziel, M.S.; Tiner, P.F.; Williams, J.K.
1993-02-01
A surface radiological investigation at the 137 Cs- and 6O Co-contaminated forest area (Chestnut Ridge east and west plots) was conducted from January 1992 through August 1992. Results of the survey revealed numerous spots and small areas of surface contamination that followed the original placement of feeders used for 6O Co- and 137 Cs-labeled seeds in a 1969--1970 study. Surface gamma exposure rates reached 380 μR/h at the east plot and 400 μR/h at the west plot, but approximately one-half and one- third, respectively, of the identified anomalies did not exceed 39 μR/h. Results of soil sample analyses demonstrated that 137 Cs and 6O Co were responsible for the elevated radiation levels. Radionuclides were found below the surface at soil sample locations, in some cases at depths below 18 in. The same pattern of subsurface contamination may be present at other elevated surface spots at both plots. These survey results show that current radiological conditions at the site remain an environmental problem. Recommendations for corrective actions are included
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Energy Technology Data Exchange (ETDEWEB)
Hersman, A; Leege, P F.A. de; Hoogenboom, J E
1992-04-01
The FORTRAN-77 program SCALPLO is being developed to make an easy and quick graphic survey of flux and/or power data calculated with SCALE modules or other core calculation or shielding codes. The basic plot functions it can perform are one- and two-dimensional plots of flux or power distributions and flux energy spectra. More specifically it can produce plots of the flux distribution in a one-dimensional geometry for one or more energy groups in one figure. It can also plot the flux distribution along a cut through a two- or three-dimensional geometry along one of the coordinate axes and it can plot a two-dimensional view of the flux distribution of a two-dimensional geometry or of a plane cut through a three-dimensional geometry. The same can be done for the power distribution in a system. Furthermore SCALPLO can plot the particle flux spectrum as a function of energy, either as group fluxes or as group fluxes per unit energy or per unit lethargy. (orig./HP).
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Litterfall and Leaf Area Index in the CONECOFOR Permanent Monitoring Plots
Directory of Open Access Journals (Sweden)
Andrea CUTINI
2002-09-01
Full Text Available Forest canopies are more sensitive and react more promptly to abiotic and biotic disturbances than other stand structural components. Monitoring crown and canopy characteristics is therefore a crucial issue for intensive and continuous monitoring programs of forest ecosystem status. These observations formed the basis for the measurement of annual litter production and leaf area index (LAI in the Italian permanent monitoring plots (CONECOFOR program established within the EC-UN/ECE program "Intensive Monitoring (Level II of Forest Ecosystems". Preliminary results after three years of observation are presented. The low value of within plot mean relative standard deviation (20.8 ± 1.9% of litter production, which in any case never exceeded 30%, accounted for the good sampling error and accuracy of the chosen method, which seems to be accurate enough to detect changes in litter production through the years. The higher inconsistency of the amount of woody and fruits fractions over the years demonstrated the greater reliability of leaf fraction or, on the other hand, of LAI compared to total litter. Mean values of annual leaf-litter and total litter production and LAI were rather high in comparison with data reported in literature for similar stands, and reflected both a medium-high productivity and a juvenile phase in the development of the selected stands on average. Focusing on changes in litter production through the years, statistical analysis on a sub-sample of plots showed the existence of significant differences both in leaf litter and total litter production. These findings seem to attribute to the "year" factor a driving role in determining changes in litter production and LAI. Temporal intermittence in data collection, together with the shortness of the monitoring period, make it difficult to speculate or arrive at definitive conclusions on changes in litter production due to time-dependent factors. The importance of having a complete
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Measurement of the ω→π+π−π0 Dalitz plot distribution
Directory of Open Access Journals (Sweden)
P. Adlarson
2017-07-01
Full Text Available Using the production reactions pd→He3ω and pp→ppω, the Dalitz plot distribution for the ω→π+π−π0 decay is studied with the WASA detector at COSY, based on a combined data sample of (4.408±0.042×104 events. The Dalitz plot density is parametrised by a product of the P-wave phase space and a polynomial expansion in the normalised polar Dalitz plot variables Z and ϕ. For the first time, a deviation from pure P-wave phase space is observed with a significance of 4.1σ. The deviation is parametrised by a linear term 1+2αZ, with α determined to be +0.147±0.036, consistent with the expectations of ρ-meson-type final-state interactions of the P-wave pion pairs.
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Dispersion-theoretical analysis of the D + → K - π + π + Dalitz plot
Niecknig, Franz; Kubis, Bastian
2015-10-01
We study the Dalitz plot of the Cabibbo-favored charmed-meson decay D + → K -π+π+ using dispersion theory. The formalism respects all constraints from analyticity and unitarity, and consistently describes final-state interactions between all three decay products. We employ pion-pion and pion-kaon phase shifts as input, and fit the pertinent subtraction constants to Dalitz plot data by the CLEO and FOCUS collaborations. Phase motions of resonant as well as nonresonant amplitudes are discussed, which should provide crucial input for future studies of CP violation in similar three-body charm decays.
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Directory of Open Access Journals (Sweden)
Kuswata Kartawinata
2003-12-01
Full Text Available KARTAWINATA, KUSWATA; SAMSOEDIN, ISMAYADI; HERIYANTO, M. AND AFRIASTINI, J. J. 2004. A tree species inventory in a one-hectare plot at the Batang Gadis National Park, North Sumatra, Indonesia. Reinwardtia 12 (2: 145 – 157. The results of the inventory of trees with DBH ≥ 10 cm shows that 184 species in 41 families, represented by 583 individuals with the total basal areas of 40.56 m² occurred in the one-hectare plot sampled. Together with the saplings and shrubs the number of species was 240 belonging to 47 families. The forest is richer in tree species than other lowland forests in North Sumatra, but poorer than those in Borneo and the Malay Peninsula. Dipterocarps constituted 18.42 % of total species with basal area of 18.99 m² or 46.82 % of the total basal area in the plot. The most prominent species was Shorea gibbosa. Hopea nigra, reported to be rare in Bangka and Belitung, occurred here as one of the ten leading species. The species-area curve shows that a considerable number of additional species was encountered more or less steadily up to one hectare and there was no indication of levelling off. A simulated profile diagram shows the forest may be stratified into five layers: (1 emergent layer, (2 upper canopy, (3 middle canopy, (4 lower canopy and (5 ground canopy. Dipterocarps were leading species in the emergent layer, upper canopy and middle canopy. Only 82 species were regenerating as represented by their presence in the sapling stage ranging from 5 to 50 plants/hectare. Macaranga lowii King ex Hook. f. dominated the section which seemed to be previously occupied by gaps.
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An automatized frequency analysis for vine plot detection and delineation in remote sensing
Delenne , Carole; Rabatel , G.; Deshayes , M.
2008-01-01
The availability of an automatic tool for vine plot detection, delineation, and characterization would be very useful for management purposes. An automatic and recursive process using frequency analysis (with Fourier transform and Gabor filters) has been developed to meet this need. This results in the determination of vine plot boundary and accurate estimation of interrow width and row orientation. To foster large-scale applications, tests and validation have been carried out on standard ver...
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Li, Huili; Ostermann, Anne; Karunarathna, Samantha C; Xu, Jianchu; Hyde, Kevin D; Mortimer, Peter E
2018-07-01
The species-area relationship is an important factor in the study of species diversity, conservation biology, and landscape ecology. A deeper understanding of this relationship is necessary, in order to provide recommendations on how to improve the quality of data collection on macrofungal diversity in different land use systems in future studies, a systematic assessment of methodological parameters, in particular optimal plot sizes. The species-area relationship of macrofungi in tropical and temperate climatic zones and four different land use systems were investigated by determining the macrofungal species richness in plot sizes ranging from 100 m 2 to 10 000 m 2 over two sampling seasons. We found that the effect of plot size on recorded species richness significantly differed between land use systems with the exception of monoculture systems. For both climate zones, land use system needs to be considered when determining optimal plot size. Using an optimal plot size was more important than temporal replication (over two sampling seasons) in accurately recording species richness. Copyright © 2018 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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Hsu, Che-Hao; Tsai, Ming-Ya; Huang, Go-Shine; Lin, Tso-Chou; Chen, Kuen-Pao; Ho, Shung-Tai; Shyu, Liang-Yu; Li, Chi-Yuan
2012-03-01
Beat-to-beat heart rate variability (HRV) is caused by the fluctuating balance of sympathetic and parasympathetic tone. The Poincaré plot has been used to evaluate HRV. In this study, we validate that this new method may qualitatively and quantitatively assess the sympathovagal fluctuation in patients during induction of anesthesia with sevoflurane. Twenty-eight young patients were allocated for the study. The patients received a tilt test and on the next day they sustained anesthesia induced with inhaled anesthetics. Electrocardiography signals from the patients were relayed to an analogue-digital converter. The Poincaré plot is quantified by measuring SD1, SD2, and SD1/SD2. Power spectral analyses were performed and LF, HF and HF/LF were calculated. The LF power and the SD2 of the Poincaré plot increased while subjects were tilt-up from the supine position. Additionally, a significant correlation were found between LF and SD2, HF and SD1 (p plot respectively. However, the LF, SD2 and LF/HF increased; the HF, SD1 and SD1/SD2 ratio decreased after intubation stimulation. Poincaré plot and power spectral analysis of HRV during tilt test and sevoflurane induction significantly correlate. Poincaré plot analysis is easier and more sensitive at evaluating the sympathovagal balance and observing the beat-to-beat HRV. Copyright © 2012. Published by Elsevier B.V.
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The impact of distance to the farm compound on the options for use of the cereal plot
Directory of Open Access Journals (Sweden)
K. TAMM
2008-12-01
Full Text Available In increasingly competitive conditions, the dominant trend of enlarging the production area of farms is causing a growth in transportation costs making the profitability of cultivating distant plots questionable. The aim of this study was to provide a method to evaluate the rationality of using a plot depending on its distance, area and cultivation technology. An algorithm and a mathematical model were composed to calculate the total costs depending on the distance to the plot. The transportation costs of machines and materials, cost of organisational travel and timeliness costs are taken into account in the model to enable determination of the maximum distance or the minimum area of the plot necessary for profitable cultivation. Simulations allow us to conclude that the growth in yield and selling price of the production allow an increase in the limit value of driving costs and, thus, the profitable distance of the plot; on the other hand, it means also an increase of timeliness costs as a limitation for extending distance. Exploitation of more distant plots can be uneconomical in coming years because of increasing fuel costs.;
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Using contour plots in elecgroproduction to examine regions in {epsilon}, Q{sup 2}, W space
Energy Technology Data Exchange (ETDEWEB)
Funsten, H. [College of William and Mary, Williamsburg, VA (United States)
1994-04-01
In determining incident CEBAF beam energies for CLAS electroproduction experiments that separate the longitudinal and transverse cross section components, contour plots of {epsilon} defined over a 2 dimensional Q{sup 2}, W space can be useful. This note describes an approximate method of constructing such plots.
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The Wally plot approach to assess the calibration of clinical prediction models.
Blanche, Paul; Gerds, Thomas A; Ekstrøm, Claus T
2017-12-06
A prediction model is calibrated if, roughly, for any percentage x we can expect that x subjects out of 100 experience the event among all subjects that have a predicted risk of x%. Typically, the calibration assumption is assessed graphically but in practice it is often challenging to judge whether a "disappointing" calibration plot is the consequence of a departure from the calibration assumption, or alternatively just "bad luck" due to sampling variability. We propose a graphical approach which enables the visualization of how much a calibration plot agrees with the calibration assumption to address this issue. The approach is mainly based on the idea of generating new plots which mimic the available data under the calibration assumption. The method handles the common non-trivial situations in which the data contain censored observations and occurrences of competing events. This is done by building on ideas from constrained non-parametric maximum likelihood estimation methods. Two examples from large cohort data illustrate our proposal. The 'wally' R package is provided to make the methodology easily usable.
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Texture Analysis of Recurrence Plots Based on Wavelets and PSO for Laryngeal Pathologies Detection.
Souza, Taciana A; Vieira, Vinícius J D; Correia, Suzete E N; Costa, Silvana L N C; de A Costa, Washington C; Souza, Micael A
2015-01-01
This paper deals with the discrimination between healthy and pathological speech signals using recurrence plots and wavelet transform with texture features. Approximation and detail coefficients are obtained from the recurrence plots using Haar wavelet transform, considering one decomposition level. The considered laryngeal pathologies are: paralysis, Reinke's edema and nodules. Accuracy rates above 86% were obtained by means of the employed method.
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Directory of Open Access Journals (Sweden)
A. Budishchev
2014-09-01
Full Text Available Most plot-scale methane emission models – of which many have been developed in the recent past – are validated using data collected with the closed-chamber technique. This method, however, suffers from a low spatial representativeness and a poor temporal resolution. Also, during a chamber-flux measurement the air within a chamber is separated from the ambient atmosphere, which negates the influence of wind on emissions. Additionally, some methane models are validated by upscaling fluxes based on the area-weighted averages of modelled fluxes, and by comparing those to the eddy covariance (EC flux. This technique is rather inaccurate, as the area of upscaling might be different from the EC tower footprint, therefore introducing significant mismatch. In this study, we present an approach to validate plot-scale methane models with EC observations using the footprint-weighted average method. Our results show that the fluxes obtained by the footprint-weighted average method are of the same magnitude as the EC flux. More importantly, the temporal dynamics of the EC flux on a daily timescale are also captured (r2 = 0.7. In contrast, using the area-weighted average method yielded a low (r2 = 0.14 correlation with the EC measurements. This shows that the footprint-weighted average method is preferable when validating methane emission models with EC fluxes for areas with a heterogeneous and irregular vegetation pattern.
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Review article: The mountain motif in the plot of Matthew
Directory of Open Access Journals (Sweden)
Gert J. Volschenk
2010-09-01
Full Text Available This article reviewed T.L. Donaldson’s book, Jesus on the mountain: A study in Matthean theology, published in 1985 by JSOT Press, Sheffield, and focused on the mountain motif in the structure and plot of the Gospel of Matthew, in addition to the work of Donaldson on the mountain motif as a literary motif and as theological symbol. The mountain is a primary theological setting for Jesus’ ministry and thus is an important setting, serving as one of the literary devices by which Matthew structured and progressed his narrative. The Zion theological and eschatological significance and Second Temple Judaism serve as the historical and theological background for the mountain motif. The last mountain setting (Mt 28:16–20 is the culmination of the three theological themes in the plot of Matthew, namely Christology, ecclesiology and salvation history.
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Common angle plots as perception-true visualizations of categorical associations.
Hofmann, Heike; Vendettuoli, Marie
2013-12-01
Visualizations are great tools of communications-they summarize findings and quickly convey main messages to our audience. As designers of charts we have to make sure that information is shown with a minimum of distortion. We have to also consider illusions and other perceptual limitations of our audience. In this paper we discuss the effect and strength of the line width illusion, a Muller-Lyer type illusion, on designs related to displaying associations between categorical variables. Parallel sets and hammock plots are both affected by line width illusions. We introduce the common-angle plot as an alternative method for displaying categorical data in a manner that minimizes the effect from perceptual illusions. Results from user studies both highlight the need for addressing line-width illusions in displays and provide evidence that common angle charts successfully resolve this issue.
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PLOTnFIT: A BASIC program for data plotting and curve fitting
Energy Technology Data Exchange (ETDEWEB)
Schiffgens, J O
1989-10-01
PLOTnFIT is a BASIC program to be used with an IBM or IBM-compatible personal computer (PC) for plotting and fitting curves to measured or observed data for both extrapolation and interpolation. It uses the Least Squares method to calculate the coefficients of nth degree polynomials (e.g., up to 10th degree) of Basis Functions so that each polynomial fits the data in a Least Squares sense, then plots the data and the polynomial that a user decides best represents them. PLOTnFIT is very versatile. It can be used to generate linear, semilog, and log-log graphs and can automatically scale the coordinate axes to suit the data. It can plot more than one data set on a graph (e.g., up to 8 data sets) and more data points than a user is likely to put on one graph (e.g., up to 225 points). A PC diskette containing (1) READIST.PNF (a summary of this NUREG), (2) INI06891.SIS and FOL06891.SIS (two data files), and 3) PLOTNFIT.4TH (the latest version of the program) may be obtained from the National Energy Software Center, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439. (author)
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Chen, Wei-Shing
2011-04-01
The aim of the article is to answer the question if the Taiwan unemployment rate dynamics is generated by a non-linear deterministic dynamic process. This paper applies a recurrence plot and recurrence quantification approach based on the analysis of non-stationary hidden transition patterns of the unemployment rate of Taiwan. The case study uses the time series data of the Taiwan’s unemployment rate during the period from 1978/01 to 2010/06. The results show that recurrence techniques are able to identify various phases in the evolution of unemployment transition in Taiwan.
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Omitted Variable Sensitivity Analysis with the Annotated Love Plot
Hansen, Ben B.; Fredrickson, Mark M.
2014-01-01
The goal of this research is to make sensitivity analysis accessible not only to empirical researchers but also to the various stakeholders for whom educational evaluations are conducted. To do this it derives anchors for the omitted variable (OV)-program participation association intrinsically, using the Love plot to present a wide range of…
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International Nuclear Information System (INIS)
Wei, Chuang; Dong, Jingyan
2013-01-01
This paper presents the direct three-dimensional (3D) fabrication of polymer scaffolds with sub-10 µm structures using electrohydrodynamic jet (EHD-jet) plotting of melted thermoplastic polymers. Traditional extrusion-based fabrication approaches of 3D periodic porous structures are very limited in their resolution, due to the excessive pressure requirement for extruding highly viscous thermoplastic polymers. EHD-jet printing has become a high-resolution alternative to other forms of nozzle deposition-based fabrication approaches by generating micro-scale liquid droplets or a fine jet through the application of a large electrical voltage between the nozzle and the substrate. In this study, we successfully apply EHD-jet plotting technology with melted biodegradable polymer (polycaprolactone, or PCL) for the fabrication of 2D patterns and 3D periodic porous scaffold structures in potential tissue engineering applications. Process conditions (e.g. electrical voltage, pressure, plotting speed) have been thoroughly investigated to achieve reliable jet printing of fine filaments. We have demonstrated for the first time that the EHD-jet plotting process is capable of the fabrication of 3D periodic structures with sub-10 µm resolution, which has great potential in advanced biomedical applications, such as cell alignment and guidance. (paper)
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Crop residue decomposition in Minnesota biochar amended plots
S. L. Weyers; K. A. Spokas
2014-01-01
Impacts of biochar application at laboratory scales are routinely studied, but impacts of biochar application on decomposition of crop residues at field scales have not been widely addressed. The priming or hindrance of crop residue decomposition could have a cascading impact on soil processes, particularly those influencing nutrient availability. Our objectives were to evaluate biochar effects on field decomposition of crop residue, using plots that were amended with ...
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Bonert, Michael; El-Shinnawy, Ihab; Carvalho, Michael; Williams, Phillip; Salama, Samih; Tang, Damu; Kapoor, Anil
2017-01-01
Observational data and funnel plots are routinely used outside of pathology to understand trends and improve performance. Extract diagnostic rate (DR) information from free text surgical pathology reports with synoptic elements and assess whether inter-rater variation and clinical history completeness information useful for continuous quality improvement (CQI) can be obtained. All in-house prostate biopsies in a 6-year period at two large teaching hospitals were extracted and then diagnostically categorized using string matching, fuzzy string matching, and hierarchical pruning. DRs were then stratified by the submitting physicians and pathologists. Funnel plots were created to assess for diagnostic bias. 3,854 prostate biopsies were found and all could be diagnostically classified. Two audits involving the review of 700 reports and a comparison of the synoptic elements with the free text interpretations suggest a categorization error rate of 40 cases and together assessed 3,690 biopsies. There was considerable inter-rater variability and a trend toward more World Health Organization/International Society of Urologic Pathology Grade 1 cancers in older pathologists. Normalized deviations plots, constructed using the median DR, and standard error can elucidate associated over- and under-calls for an individual pathologist in relation to their practice group. Clinical history completeness by submitting medical doctor varied significantly (100% to 22%). Free text data analyses have some limitations; however, they could be used for data-driven CQI in anatomical pathology, and could lead to the next generation in quality of care.
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Zhou, Yun; Sojkova, Jitka; Resnick, Susan M.; Wong, Dean F.
2012-01-01
Both the standardized uptake value ratio (SUVR) and the Logan plot result in biased distribution volume ratios (DVR) in ligand-receptor dynamic PET studies. The objective of this study is to use a recently developed relative equilibrium-based graphical plot (RE plot) method to improve and simplify the two commonly used methods for quantification of [11C]PiB PET. Methods The overestimation of DVR in SUVR was analyzed theoretically using the Logan and the RE plots. A bias-corrected SUVR (bcSUVR) was derived from the RE plot. Seventy-eight [11C]PiB dynamic PET scans (66 from controls and 12 from mildly cognitively impaired participants (MCI) from the Baltimore Longitudinal Study of Aging (BLSA)) were acquired over 90 minutes. Regions of interest (ROIs) were defined on coregistered MRIs. Both the ROI and pixelwise time activity curves (TACs) were used to evaluate the estimates of DVR. DVRs obtained using the Logan plot applied to ROI TACs were used as a reference for comparison of DVR estimates. Results Results from the theoretical analysis were confirmed by human studies. ROI estimates from the RE plot and the bcSUVR were nearly identical to those from the Logan plot with ROI TACs. In contrast, ROI estimates from DVR images in frontal, temporal, parietal, cingulate regions, and the striatum were underestimated by the Logan plot (controls 4 – 12%; MCI 9 – 16%) and overestimated by the SUVR (controls 8 – 16%; MCI 16 – 24%). This bias was higher in the MCI group than in controls (p plot or the bcSUVR. Conclusion The RE plot improves pixel-wise quantification of [11C]PiB dynamic PET compared to the conventional Logan plot. The bcSUVR results in lower bias and higher consistency of DVR estimates compared to SUVR. The RE plot and the bcSUVR are practical quantitative approaches that improve the analysis of [11C]PiB studies. PMID:22414634
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Brains striving for coherence: Long-term cumulative plot formation in the default mode network.
Tylén, K; Christensen, P; Roepstorff, A; Lund, T; Østergaard, S; Donald, M
2015-11-01
Many everyday activities, such as engaging in conversation or listening to a story, require us to sustain attention over a prolonged period of time while integrating and synthesizing complex episodic content into a coherent mental model. Humans are remarkably capable of navigating and keeping track of all the parallel social activities of everyday life even when confronted with interruptions or changes in the environment. However, the underlying cognitive and neurocognitive mechanisms of such long-term integration and profiling of information remain a challenge to neuroscience. While brain activity is generally traceable within the short time frame of working memory (milliseconds to seconds), these integrative processes last for minutes, hours or even days. Here we report two experiments on story comprehension. Experiment I establishes a cognitive dissociation between our comprehension of plot and incidental facts in narratives: when episodic material allows for long-term integration in a coherent plot, we recall fewer factual details. However, when plot formation is challenged, we pay more attention to incidental facts. Experiment II investigates the neural underpinnings of plot formation. Results suggest a central role for the brain's default mode network related to comprehension of coherent narratives while incoherent episodes rather activate the frontoparietal control network. Moreover, an analysis of cortical activity as a function of the cumulative integration of narrative material into a coherent story reveals to linear modulations of right hemisphere posterior temporal and parietal regions. Together these findings point to key neural mechanisms involved in the fundamental human capacity for cumulative plot formation. Copyright © 2015 Elsevier Inc. All rights reserved.
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36 CFR 9.42 - Well records and reports, plots and maps, samples, tests and surveys.
2010-07-01
... Well records and reports, plots and maps, samples, tests and surveys. Any technical data gathered... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Well records and reports, plots and maps, samples, tests and surveys. 9.42 Section 9.42 Parks, Forests, and Public Property...
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From research plots to prototype biomass plantations
Energy Technology Data Exchange (ETDEWEB)
Kenney, W.A.; Vanstone, B.J.; Gambles, R.L.; Zsuffa, L. [Univ. of Toronto, Ontario (Canada)
1993-12-31
The development of biomass energy plantations is now expanding from the research plot phase into the next level of development at larger scale plantings. This is necessary to provide: more accurate information on biomass yields, realistic production cost figures, venues to test harvesting equipment, demonstration sites for potential producers, and a supply of feedstock for prototype conversion facilities. The paper will discuss some of these objectives and some of the challenges encountered in the scale-up process associated with a willow prototype plantation project currently under development in Eastern Canada.
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Computational Drafting of Plot Structures for Russian Folk Tales.
Gervás, Pablo
The plots of stories are known to follow general patterns in terms of their overall structure. This was the basic tenet of structuralist approaches to narratology. Vladimir Propp proposed a procedure for the generation of new tales based on his semi-formal description of the structure of Russian folk tales. This is one of the first existing instances of a creative process described procedurally. The present paper revisits Propp's morphology to build a system that generates instances of Russian folk tales. Propp's view of the folk tale as a rigid sequence of character functions is employed as a plot driver, and some issues that Propp declared relevant but did not explore in detail-such as long-range dependencies between functions or the importance of endings-are given computational shape in the context of a broader architecture that captures all the aspects discussed by Propp. A set of simple evaluation metrics for the resulting outputs is defined inspired on Propp's formalism. The potential of the resulting system for providing a creative story generation system is discussed, and possible lines of future work are discussed.
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Graffelman, J.; Eeuwijk, van F.A.
2005-01-01
The scatter plot is a well known and easily applicable graphical tool to explore relationships between two quantitative variables. For the exploration of relations between multiple variables, generalisations of the scatter plot are useful. We present an overview of multivariate scatter plots
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Evenly spaced Detrended Fluctuation Analysis: Selecting the number of points for the diffusion plot
Liddy, Joshua J.; Haddad, Jeffrey M.
2018-02-01
Detrended Fluctuation Analysis (DFA) has become a widely-used tool to examine the correlation structure of a time series and provided insights into neuromuscular health and disease states. As the popularity of utilizing DFA in the human behavioral sciences has grown, understanding its limitations and how to properly determine parameters is becoming increasingly important. DFA examines the correlation structure of variability in a time series by computing α, the slope of the log SD- log n diffusion plot. When using the traditional DFA algorithm, the timescales, n, are often selected as a set of integers between a minimum and maximum length based on the number of data points in the time series. This produces non-uniformly distributed values of n in logarithmic scale, which influences the estimation of α due to a disproportionate weighting of the long-timescale regions of the diffusion plot. Recently, the evenly spaced DFA and evenly spaced average DFA algorithms were introduced. Both algorithms compute α by selecting k points for the diffusion plot based on the minimum and maximum timescales of interest and improve the consistency of α estimates for simulated fractional Gaussian noise and fractional Brownian motion time series. Two issues that remain unaddressed are (1) how to select k and (2) whether the evenly-spaced DFA algorithms show similar benefits when assessing human behavioral data. We manipulated k and examined its effects on the accuracy, consistency, and confidence limits of α in simulated and experimental time series. We demonstrate that the accuracy and consistency of α are relatively unaffected by the selection of k. However, the confidence limits of α narrow as k increases, dramatically reducing measurement uncertainty for single trials. We provide guidelines for selecting k and discuss potential uses of the evenly spaced DFA algorithms when assessing human behavioral data.
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Point and Fixed Plot Sampling Inventory Estimates at the Savannah River Site, South Carolina.
Energy Technology Data Exchange (ETDEWEB)
Parresol, Bernard, R.
2004-02-01
This report provides calculation of systematic point sampling volume estimates for trees greater than or equal to 5 inches diameter breast height (dbh) and fixed radius plot volume estimates for trees < 5 inches dbh at the Savannah River Site (SRS), Aiken County, South Carolina. The inventory of 622 plots was started in March 1999 and completed in January 2002 (Figure 1). Estimates are given in cubic foot volume. The analyses are presented in a series of Tables and Figures. In addition, a preliminary analysis of fuel levels on the SRS is given, based on depth measurements of the duff and litter layers on the 622 inventory plots plus line transect samples of down coarse woody material. Potential standing live fuels are also included. The fuels analyses are presented in a series of tables.
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AN EXPERIMENTAL ASSESSMENT OF THE PLATE HEAT EXCHANGER CHARACTERISTICS BY WILSON PLOT METHOD
Directory of Open Access Journals (Sweden)
Jan Opatřil
2016-10-01
Full Text Available An aim of this paper is suggestion of the evaluation method based on the experimental data and the Wilson plot method for the Plate Heat Exchangers (PHE. For the purpose of the project the new experimental loop was built for the testing of PHE to obtain the overhaul heat transfer coefficient and pressure drop between inlet and outlet of the fluid. The measurement were done for three different PHE with the performance range 30-100kW. The working fluid was water on both sides of the PHE. The differences are in number of pates as well as in extrusion profiles. The Wilson plot evaluation method was involved for the processing experimental data. To obtain more accurate correlations between the experimental data and theoretical results yield of the Wilson plot, the method was enhanced by the measured pressure drop involving. This approach could be useful for PHE designing software and for the manufacturing company.
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A new method to study ferroelectrics using the remanent Henkel plots
Vopson, Melvin M.
2018-05-01
Analysis of experimental curves constructed from dc demagnetization and isothermal remanent magnetization known as Henkel and delta M plots, have served for over 53 years as an important tool for characterization of interactions in ferromagnets. In this article we address the question whether the same experimental technique could be applied to the study of ferroelectric systems. The successful measurement of the equivalent dc depolarisation and isothermal remanent polarization curves and the construction of the Henkel and delta P plots for ferroelectrics is reported here. Full measurement protocol is provided together with experimental examples for two ferroelectric ceramic samples. This new measurement technique is an invaluable experimental tool that could be used to further advance our understanding of ferroelectric materials and their applications.
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European Vegetation Archive (EVA): an integrated database of European vegetation plots
DEFF Research Database (Denmark)
Chytrý, M; Hennekens, S M; Jiménez-Alfaro, B
2015-01-01
vegetation- plot databases on a single software platform. Data storage in EVA does not affect on-going independent development of the contributing databases, which remain the property of the data contributors. EVA uses a prototype of the database management software TURBOVEG 3 developed for joint management......The European Vegetation Archive (EVA) is a centralized database of European vegetation plots developed by the IAVS Working Group European Vegetation Survey. It has been in development since 2012 and first made available for use in research projects in 2014. It stores copies of national and regional...... data source for large-scale analyses of European vegetation diversity both for fundamental research and nature conservation applications. Updated information on EVA is available online at http://euroveg.org/eva-database....
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A Conductive Gel for the Plotting of Equipotential Lines
Elizalde-Torres, J.; González-Cardel, M.; Vega-Murguía, E. J.; Castillo-González, I.; Rodríguez-Nava, M.
2015-01-01
This paper presents the development of a conductive gel that can be used to measure the electrical potential differences on its surface, and has enough consistency to plot equipotential lines. It has a gelation time of less than 10 min, and is suitable for implementing learning experiences in a physics teaching laboratory in a 90 min session. To…
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Hiwatashi, Akio; Togao, Osamu; Yamashita, Koji; Kikuchi, Kazufumi; Yoshimoto, Koji; Mizoguchi, Masahiro; Suzuki, Satoshi O; Yoshiura, Takashi; Honda, Hiroshi
2016-07-01
Correction of contrast leakage is recommended when enhancing lesions during perfusion analysis. The purpose of this study was to assess the diagnostic performance of computed tomography perfusion (CTP) with a delay-invariant singular-value decomposition algorithm (SVD+) and a Patlak plot in differentiating glioblastomas from lymphomas. This prospective study included 17 adult patients (12 men and 5 women) with pathologically proven glioblastomas (n=10) and lymphomas (n=7). CTP data were analyzed using SVD+ and a Patlak plot. The relative tumor blood volume and flow compared to contralateral normal-appearing gray matter (rCBV and rCBF derived from SVD+, and rBV and rFlow derived from the Patlak plot) were used to differentiate between glioblastomas and lymphomas. The Mann-Whitney U test and receiver operating characteristic (ROC) analyses were used for statistical analysis. Glioblastomas showed significantly higher rFlow (3.05±0.49, mean±standard deviation) than lymphomas (1.56±0.53; P0.05), rCBF (1.38±0.41 vs. 1.29±0.47; P>0.05), or rCBV (1.78±0.47 vs. 1.87±0.66; P>0.05). ROC analysis showed the best diagnostic performance with rFlow (Az=0.871), followed by rBV (Az=0.771), rCBF (Az=0.614), and rCBV (Az=0.529). CTP analysis with a Patlak plot was helpful in differentiating between glioblastomas and lymphomas, but CTP analysis with SVD+ was not. Copyright © 2016 Elsevier Masson SAS. All rights reserved.