WorldWideScience

Sample records for line probe technology

  1. Probe-based recording technology

    International Nuclear Information System (INIS)

    Naberhuis, Steve

    2002-01-01

    The invention of the scanning tunneling microscope (STM) prompted researchers to contemplate whether such technology could be used as the basis for the storage and retrieval of information. With magnetic data storage technology facing limits in storage density due to the thermal instability of magnetic bits, the super-paramagnetic limit, the heir-apparent for information storage at higher densities appeared to be variants of the STM or similar probe-based storage techniques such as atomic force microscopy (AFM). Among these other techniques that could provide replacement technology for magnetic storage, near-field optical scanning optical microscopy (NSOM or SNOM) has also been investigated. Another alternative probe-based storage technology called atomic resolution storage (ARS) is also currently under development. An overview of these various technologies is herein presented, with an analysis of the advantages and disadvantages inherent in each particularly with respect to reduced device dimensions. The role of micro electro mechanical systems (MEMS) is emphasized

  2. Corrosion probe. Innovative technology summary report

    International Nuclear Information System (INIS)

    1999-05-01

    Over 253 million liters of high-level waste (HLW) generated from plutonium production is stored in mild steel tanks at the Department of Energy (DOE) Hanford Site. Corrosion monitoring of double-shell storage tanks (DSTs) is currently performed at Hanford using a combination of process knowledge and tank waste sampling and analysis. Available technologies for corrosion monitoring have progressed to a point where it is feasible to monitor and control corrosion by on-line monitoring of the corrosion process and direct addition of corrosion inhibitors. The electrochemical noise (EN) technique deploys EN-based corrosion monitoring probes into storage tanks. This system is specifically designed to measure corrosion rates and detect changes in waste chemistry that trigger the onset of pitting and cracking. These on-line probes can determine whether additional corrosion inhibitor is required and, if so, provide information on an effective end point to the corrosion inhibitor addition procedure. This report describes the technology, its performance, its application, costs, regulatory and policy issues, and lessons learned

  3. Rf probe technology for the next generation of technological plasmas

    International Nuclear Information System (INIS)

    Law, V.J.; Kenyon, A.J.; Thornhill, N.F.; Seeds, A.J.; Batty, I.

    2001-01-01

    We describe radio frequency (rf) analysis of technological plasmas at the 13.56 MHz fundamental drive frequency and integer narrow-band harmonics up to n = 9. In particular, we demonstrate the use of harmonic amplitude information as a process end-point diagnostic. Using very high frequency (vhf) techniques, we construct non-invasive ex situ remote-coupled probes: a diplexer, an equal-ratio-arm bridge, and a dual directional coupler used as a single directional device. These probes bolt into the plasma-tool 50 Ω transmission-line between the rf generator and matching network, and hence do not require modification of the plasma tool. The 50 Ω probe environment produces repeatable measurements of the chamber capacitance and narrow-band harmonic amplitude with an end-point detection sensitivity corresponding to a 2 dB change in the harmonic amplitude with the removal of 1 cm 2 of photoresist. The methodology and design of an instrument for the measurement of the plasma-tool frequency response, and the plasma harmonic amplitude and phase response are examined. The instrument allows the monitoring of the plasma phase delay, plasma-tool short- and long-term ageing, and process end-point prediction. (author)

  4. Raman probe. Innovative technology summary report

    International Nuclear Information System (INIS)

    1999-07-01

    The Raman probe is deployed in high-level waste tanks with the cone penetrometer (CPT). These technologies are engineered and optimized to work together. All of the hardware is radiation hardened, designed for and tested in the high-radiation, highly caustic chemical environment of US Department of Energy's (DOE's) waste storage tanks. When deployed in tanks, the system is useful for rapidly assessing the species and concentrations of organic-bearing tank wastes. The CPT was originally developed for geological and groundwater applications, with sensors that measure physical parameters such as soil moisture, temperature, and pH. When deployed, it is hydraulically forced directly into the ground rather than using boring techniques utilized by rotary drilling systems. There is a separate Innovative Technology Summary Report for the CPT, so this report will focus on the changes made specifically to support the Raman probe. The most significant changes involve adapting the Raman probe for in-tank and subsurface field use and developing meaningful real-time data analysis. Testing of the complete LLNL system was conducted in a hot cell in the 222-S Laboratory at the Hanford site in summer 1997. Both instruments were tested in situ on solvent-contaminated soils (TCE and PCE) at the Savannah River Site in February and June 1998. This report describes the technology, its performance, its uses, cost, regulatory and policy issues, and lessons learned

  5. Technology Management within Product Lines in High Technology Markets

    Science.gov (United States)

    Sarangee, Kumar R.

    2009-01-01

    Understanding the nuances of product line management has been of great interest to business scholars and practitioners. This assumes greater significance for firms conducting business in technologically dynamic industries, where they face certain challenges regarding the management of multiple, overlapping technologies within their product lines.…

  6. Molecular Probes: An Innovative Technology for Monitoring Membrane Processes

    Science.gov (United States)

    Santoro, Sergio

    The ultimate objective of this study is to use molecular probes as an innovative and alternative technology contributing to the advance of membrane science by monitoring membrane processes in-situ, on-line and at sub-micron scale. An optical sensor for oxygen sensing was developed by the immobilization of tris (1,10-phenanthroline) ruthenium (II) (Ru(phen)3) in a dense polymeric membrane made of polystyrene (PS) or Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The emission of the probe was quenched by both the temperature and by the oxygen. Moreover, the oxygen sensitivity was affected by the oxygen permeability of the membrane. The evaluation of the oxygen concentration is prone to errors since the emission of a single probe depends on several parameters (i.e. optical path, source intensity). The correction of these artefacts was obtained by the immobilization of a second luminescent molecule non-sensitive to the oxygen, Coumarin. The potential of the luminescent ratiometric sensor for the non-invasive monitoring of oxygen in food packaging using polymeric films with different oxygen permeability was evaluated. Emphasis was given to the efficiency of the optical sensor for the on-line, in-situ and non invasive monitoring of the oxygen by comparing the experimental data with a model which takes into account the oxygen permeability of the packaging materials evaluated independently. A nano-thermometer based on silica nano-particles doped with Ru(phen)3 was developed. A systematic study shows how it is possible to control the properties of the nano-particles as well as their temperature sensitivity. The nano-thermometer was immobilized on a membrane surface by dip-coating providing information about the temperature on the membrane surface. Hydrophobic porous membrane made of Poly(vinylidene fluoride) was prepared via electrospinning and employed in a direct contact membrane distillation process. Using a designed membrane module and a membrane doped with Ru

  7. On-line biofilm monitoring by "BIOX" electrochemical probe.

    Science.gov (United States)

    Mollica, A; Cristiani, P

    2003-01-01

    The innovative electrochemical monitoring probe (BIOX) recently developed to improve the antifouling treatments of cooling systems in industrial plants is presented. On the basis of the good results obtained from applications on marine sites, some research has been stated to validate this technique in biofilm growth and prevention of microbial corrosion in fresh and drinking waters.

  8. Brief overview of geophysical probing technology

    International Nuclear Information System (INIS)

    Ramirez, A.L.; Lytle, R.J.

    1982-01-01

    An evaluation of high-resolution geophysical techniques which can be used to characterize a nulcear waste disposal site is being conducted by the Lawrence Livermore National Laboratory (LLNL) at the request of the US Nuclear Regulatory Commisson (NRC). LLNL is involved in research work aimed at evaluating the current capabilities and limitations of geophysical methods used for site selection. This report provides a brief overview of the capabilities and limitations associated with this technology and explains how our work addresses some of the present limitations. We are examining both seismic and electromagnetic techniques to obtain high-resolution information. We are also assessing the usefulness of geotomography in mapping fracture zones remotely. Finally, we are collecting core samples from a site in an effort to assess the capability of correlating such geophysical data with parameters of interest such as fracture continuity, orientation, and fracture density

  9. Probing the N = Z Line via β Decay

    International Nuclear Information System (INIS)

    Markku Oinonen

    1999-01-01

    This contribution reports several beta-decay studies performed at ISOLDE On-Line Mass Separator at CERN recently for nuclei close to N = Z line. Beta decay of 58 Zn provides a possibility to compare Gamow-Teller strength extracted from complementary beta-decay studies and charge-exchange reactions. Measurement on beta-decay half-life of 70 Kr shows importance of experimental information in modeling the path of the astrophysical rp process. Decay of 71 Kr is an example of a mirror beta decay and extends the systematics of these particular decays towards highly deformed region close to A = 80

  10. Magnetic Probe Construction using Thick-film Technology

    International Nuclear Information System (INIS)

    Takahashi, H.; Sakakibara, S.; Kubota, Y.; Yamada, H.

    2001-01-01

    Thick-film technology has been successfully adapted for the design and fabrication of magnetic probes of a new type suitable for use in the simultaneous ultra-high vacuum and high-temperature environment of a nuclear fusion device. The maximum usable temperature is expected to be around 900 degrees C. This new probe has a specific sensitivity (coupling area per unit volume) an order of magnitude higher than a conventional coil. The new probe in one implementation is capable of simultaneously measuring magnetic field in three orthogonal directions about a single spatial point and in two frequency ranges. Low-frequency coils have a measured coupling area of 296-323 cm squared and a frequency response of about 300 kHz. High-frequency coils have a design coupling area of 12-15 cm squared

  11. SNOW LINES AS PROBES OF TURBULENT DIFFUSION IN PROTOPLANETARY DISKS

    International Nuclear Information System (INIS)

    Owen, James E.

    2014-01-01

    Sharp chemical discontinuities can occur in protoplanetary disks, particularly at ''snow lines'' where a gas-phase species freezes out to form ice grains. Such sharp discontinuities will diffuse out due to the turbulence suspected to drive angular momentum transport in accretion disks. We demonstrate that the concentration gradient—in the vicinity of the snow line—of a species present outside a snow line but destroyed inside is strongly sensitive to the level of turbulent diffusion (provided the chemical and transport timescales are decoupled) and provides a direct measurement of the radial ''Schmidt number'' (the ratio of the angular momentum transport to radial turbulent diffusion). Taking as an example the tracer species N 2 H + , which is expected to be destroyed inside the CO snow line (as recently observed in TW Hya) we show that ALMA observations possess significant angular resolution to constrain the Schmidt number. Since different turbulent driving mechanisms predict different Schmidt numbers, a direct measurement of the Schmidt number in accretion disks would allow inferences to be made about the nature of the turbulence

  12. Efficient design of multituned transmission line NMR probes: the electrical engineering approach.

    Science.gov (United States)

    Frydel, J A; Krzystyniak, M; Pienkowski, D; Pietrzak, M; de Sousa Amadeu, N; Ratajczyk, T; Idzik, K; Gutmann, T; Tietze, D; Voigt, S; Fenn, A; Limbach, H H; Buntkowsky, G

    2011-01-01

    Transmission line-based multi-channel solid state NMR probes have many advantages regarding the cost of construction, number of RF-channels, and achievable RF-power levels. Nevertheless, these probes are only rarely employed in solid state-NMR-labs, mainly owing to the difficult experimental determination of the necessary RF-parameters. Here, the efficient design of multi-channel solid state MAS-NMR probes employing transmission line theory and modern techniques of electrical engineering is presented. As technical realization a five-channel ((1)H, (31)P, (13)C, (2)H and (15)N) probe for operation at 7 Tesla is described. This very cost efficient design goal is a multi port single coil transmission line probe based on the design developed by Schaefer and McKay. The electrical performance of the probe is determined by measuring of Scattering matrix parameters (S-parameters) in particular input/output ports. These parameters are compared to the calculated parameters of the design employing the S-matrix formalism. It is shown that the S-matrix formalism provides an excellent tool for examination of transmission line probes and thus the tool for a rational design of these probes. On the other hand, the resulting design provides excellent electrical performance. From a point of view of Nuclear Magnetic Resonance (NMR), calibration spectra of particular ports (channels) are of great importance. The estimation of the π/2 pulses length for all five NMR channels is presented. Copyright © 2011 Elsevier Inc. All rights reserved.

  13. Advancement of Miniature Optic Gas Sensor (MOGS) Probe Technology

    Science.gov (United States)

    Chullen, Cinda

    2015-01-01

    Advancement of Miniature Optic Gas Sensor (MOGS) Probe Technology" project will investigate newly developed optic gas sensors delivered from a Small Business Innovative Research (SBIR) Phase II effort. A ventilation test rig will be designed and fabricated to test the sensors while integrated with a Suited Manikin Test Apparatus (SMTA). Once the sensors are integrated, a series of test points will be completed to verify that the sensors can withstand Advanced Suit Portable Life Support System (PLSS) environments and associated human metabolic profiles for changes in pressure and levels of Oxygen (ppO2), carbon dioxide (ppCO2), and humidity (ppH2O).

  14. On-line velocity measurements using phase probes at the SuperHILAC

    International Nuclear Information System (INIS)

    Feinberg, B.; Meaney, D.; Thatcher, R.; Timossi, C.

    1987-12-01

    Phase probes have been placed in several external beam lines at the LBL heavy ion linear accelerator (SuperHILAC) to provide non- destructive velocity measurements independent of the ion being accelerated. The system uses three probes in each line to obtain accurate velocity measurements at all beam energies. Automatic gain control and signal analysis are performed so that the energy/nucleon along with up to three probe signals are displayed on a vector graphics display with a refresh rate better than twice per second. The system uses a sensitive pseudo-correlation technique to pick out the signal from the noise, features simultaneous measurements of up to four ion velocities when more than one beam is being accelerated, and is controlled by a touch-screen operator interface. It is accurate to within /+-/0.25% and has provisions for on-line calibration tests. The phase probes thus provide a velocity measurement independent of the mass defect associated with the use of crystal detectors, which can become significant for heavy elements. They are now used as a routine tuning aid to ensure proper bunch structure, and as a beam velocity monitor. 3 refs., 5 figs

  15. Temperature dependence of dynamical permeability characterization of magnetic thin films using shorted microstrip line probe

    International Nuclear Information System (INIS)

    Li, Xiling; Li, Chengyi; Chai, Guozhi

    2017-01-01

    A temperature dependence microwave permeability characterization system of magnetic thin film up to 10 GHz is designed and fabricated. This system can be used at temperatures ranging from room temperature to 200 °C, and is based on a shorted microstrip probe, which is made by microwave printed circuit board. Without contacting the magnetic thin films to the probe, the microwave permeability of the film can be detected without any limitations of sample size and with almost the same accuracy, as shown by comparison with the results obtained from a shorted microstrip transmission-line fixture. The complex permeability can be deduced by an analytical approach from the measured reflection coefficient of a strip line ( S 11 ) with and without a ferromagnetic film material on it. The procedures are the same with the shorted microstrip transmission-line method. The microwave permeability of an oblique deposited CoZr thin film was investigated with this probe. The results show that the room temperature dynamic permeability of the CoZr film is in good agreement with the results obtained from the established short-circuited microstrip perturbation method. The temperature dependence permeability results fit well with the Landau–Lifshitz–Gilbert equation. Development of the temperature-dependent measurement of the magnetic properties of magnetic thin film may be useful for the high-frequency application of magnetic devices at high temperatures. (paper)

  16. Development of Thinopyrum ponticum-specific molecular markers and FISH probes based on SLAF-seq technology.

    Science.gov (United States)

    Liu, Liqin; Luo, Qiaoling; Teng, Wan; Li, Bin; Li, Hongwei; Li, Yiwen; Li, Zhensheng; Zheng, Qi

    2018-05-01

    Based on SLAF-seq, 67 Thinopyrum ponticum-specific markers and eight Th. ponticum-specific FISH probes were developed, and these markers and probes could be used for detection of alien chromatin in a wheat background. Decaploid Thinopyrum ponticum (2n = 10x = 70) is a valuable gene reservoir for wheat improvement. Identification of Th. ponticum introgression would facilitate its transfer into diverse wheat genetic backgrounds and its practical utilization in wheat improvement. Based on specific-locus-amplified fragment sequencing (SLAF-seq) technology, 67 new Th. ponticum-specific molecular markers and eight Th. ponticum-specific fluorescence in situ hybridization (FISH) probes have been developed from a tiny wheat-Th. ponticum translocation line. These newly developed molecular markers allowed the detection of Th. ponticum DNA in a variety of materials specifically and steadily at high throughput. According to the hybridization signal pattern, the eight Th. ponticum-specific probes could be divided into two groups. The first group including five dispersed repetitive sequence probes could identify Th. ponticum chromatin more sensitively and accurately than genomic in situ hybridization (GISH). Whereas the second group having three tandem repetitive sequence probes enabled the discrimination of Th. ponticum chromosomes together with another clone pAs1 in wheat-Th. ponticum partial amphiploid Xiaoyan 68.

  17. PROBING FUNDAMENTAL CONSTANT EVOLUTION WITH REDSHIFTED CONJUGATE-SATELLITE OH LINES

    International Nuclear Information System (INIS)

    Kanekar, Nissim; Chengalur, Jayaram N.; Ghosh, Tapasi

    2010-01-01

    We report Westerbork Synthesis Radio Telescope and Arecibo Telescope observations of the redshifted satellite OH 18 cm lines at z ∼ 0.247 toward PKS 1413+135. The 'conjugate' nature of these lines, with one line in emission and the other in absorption, but with the same shape, implies that the lines arise in the same gas. The satellite OH 18 cm line frequencies also have different dependences on the fine structure constant α, the proton-electron mass ratio μ = m p /m e , and the proton gyromagnetic ratio g p . Comparisons between the satellite line redshifts in conjugate systems can hence be used to probe changes in α, μ, and g p , with few systematic effects. The technique yields the expected null result when applied to Cen.A, a nearby conjugate satellite system. For the z ∼ 0.247 system toward PKS 1413+135, we find, on combining results from the two telescopes, that (ΔG/G) = (-1.18 ± 0.46) x 10 -5 (weighted mean), where G = g p (μα 2 ) 1.85 ; this is tentative evidence (with 2.6 σ significance, or at 99.1% confidence) for a smaller value of α, μ, and/or g p at z ∼ 0.247, i.e., at a lookback time of ∼2.9 Gyr. If we assume that the dominant change is in α, this implies (Δα/α) = (-3.1 ± 1.2) x 10 -6 . We find no evidence that the observed offset might be produced by systematic effects, either due to observational or analysis procedures, or local conditions in the molecular cloud.

  18. Advanced Technology Cloud Particle Probe for UAS, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — In Phase II SPEC will design, fabricate and flight test a state-of-the-art combined cloud particle probe called the Hawkeye. Hawkeye is the culmination of two...

  19. Comparison of Current On-line Payment Technologies

    OpenAIRE

    Mandadi, Ravi

    2006-01-01

    The purpose of this thesis work was to make a survey of current on-line payment technologies and find out which are they and how do they work? Compare and analyze them from a security point of view, as well as a usability point of view. What is good? What is bad? What is lacking? To achieve this purpose, an overview of the current on-line payment technologies was acquired through academic books and papers, Internet sites, magazines. Basic cryptographic and security related techniques were stu...

  20. Thirteen pump-probe resonances of the sodium D1 line

    International Nuclear Information System (INIS)

    Wong, Vincent; Boyd, Robert W.; Stroud, C. R. Jr.; Bennink, Ryan S.; Marino, Alberto M.

    2003-01-01

    We present the results of a pump-probe laser spectroscopic investigation of the Doppler-broadened sodium D1 resonance line. We find 13 resonances in the resulting spectra. These observations are well described by the numerical predictions of a four-level atomic model of the hyperfine structure of the sodium D1 line. We also find that many, but not all, of these features can be understood in terms of processes originating in a two-level or three-level subset of the full four-level model. The processes we observed include forward near-degenerate four-wave mixing and saturation in a two-level system, difference-frequency crossing and nondegenerate four-wave mixing in a three-level V system, electromagnetically induced transparency and optical pumping in a three-level lambda system, cross-transition resonance in a four-level double-lambda system, and conventional optical pumping. Most of these processes lead to sub-Doppler or even subnatural linewidths. The dependence of these resonances on the pump intensity and pump detuning from atomic resonance are also studied

  1. Plasma membrane organization and dynamics is probe and cell line dependent.

    Science.gov (United States)

    Huang, Shuangru; Lim, Shi Ying; Gupta, Anjali; Bag, Nirmalya; Wohland, Thorsten

    2017-09-01

    The action and interaction of membrane receptor proteins take place within the plasma membrane. The plasma membrane, however, is not a passive matrix. It rather takes an active role and regulates receptor distribution and function by its composition and the interaction of its lipid components with embedded and surrounding proteins. Furthermore, it is not a homogenous fluid but contains lipid and protein domains of various sizes and characteristic lifetimes which are important in regulating receptor function and signaling. The precise lateral organization of the plasma membrane, the differences between the inner and outer leaflet, and the influence of the cytoskeleton are still debated. Furthermore, there is a lack of comparisons of the organization and dynamics of the plasma membrane of different cell types. Therefore, we used four different specific membrane markers to test the lateral organization, the differences between the inner and outer membrane leaflet, and the influence of the cytoskeleton of up to five different cell lines, including Chinese hamster ovary (CHO-K1), Human cervical carcinoma (HeLa), neuroblastoma (SH-SY5Y), fibroblast (WI-38) and rat basophilic leukemia (RBL-2H3) cells by Imaging Total Internal Reflection (ITIR)-Fluorescence Correlation Spectroscopy (FCS). We measure diffusion in the temperature range of 298-310K to measure the Arrhenius activation energy (E Arr ) of diffusion and apply the FCS diffusion law to obtain information on the spatial organization of the probe molecules on the various cell membranes. Our results show clear differences of the FCS diffusion law and E Arr for the different probes in dependence of their localization. These differences are similar in the outer and inner leaflet of the membrane. However, these values can differ significantly between different cell lines raising the question how molecular plasma membrane events measured in different cell lines can be compared. This article is part of a Special Issue

  2. Trend on High-speed Power Line Communication Technology

    Science.gov (United States)

    Ogawa, Osamu

    High-speed power line communication (PLC) is useful technology to easily build the communication networks, because construction of new infrastructure is not necessary. In Europe and America, PLC has been used for broadband networks since the beginning of 21th century. In Japan, high-speed PLC was deregulated only indoor usage in 2006. Afterward it has been widely used for home area network, LAN in hotels and school buildings and so on. And recently, PLC is greatly concerned as communication technology for smart grid network. In this paper, the author surveys the high-speed PLC technology and its current status.

  3. The line-emitting gas in active galaxies - A probe of the nuclear engine

    Science.gov (United States)

    Veilleux, Sylvain

    1993-01-01

    This paper reviews some of the basic questions regarding the structure of the engine powering active galactic nuclei (AGN), the nature of the interaction between the AGN and the host galaxy, and the origin and evolution of AGN. The study of the dynamics and physical characteristics of the line-emitting gas in these objects has proven fruitful in addressing many of these issues. Recent advances in optical and infrared detector technology combined with the development of superior ground-based instruments have produced efficient new tools for the study of the line-emitting gas on nuclear and Galactic scales. Programs which take advantage of two of these new techniques, Fabry-Perot imaging spectroscopy and infrared spectroscopy, are described in this paper. The origin of nuclear activity in galaxies is also addressed in a third project which aims at determining the nature of luminous infrared galaxies.

  4. Development and evaluation of a long-term deposit probe for on-line monitoring of deposit growth

    Energy Technology Data Exchange (ETDEWEB)

    Brink, Anders; Lauren, Tor; Yrjas, Patrik; Hupa, Mikko [Process Chemistry Centre, Aabo Akademi University, Biskopsgatan 8, FI-20540 Turku (Finland); Friesenbichler, Joachim [Institute for Resource Efficient and Sustainable Systems, Technical University Graz Inffeldg. 21b, A-8010 Graz (Austria)

    2007-12-15

    A newly designed air-cooled probe for on-line monitoring of deposition growth has been tested in a boiler firing three woody fuels. Thermocouples are mounted on both sides of the tube wall enabling measurements of the heat flux through the probe wall. Knowing the heat flux through the probe wall, it is possible to measure the additional heat transfer resistance caused by the deposit and to estimate the properties of the deposit. Calculating the deposit thickness using the collected temperature data indicated the thinnest deposit when wood was fired, followed by bark and waste wood. The calculated deposit thickness was larger than those found when analysing the deposit thickness after the probe had been removed. Nevertheless, the ranking of fuels by deposit build-up rate was the same. (author)

  5. Line probe assay for differentiation within Mycobacterium tuberculosis complex. Evaluation on clinical specimens and isolates including Mycobacterium pinnipedii

    DEFF Research Database (Denmark)

    Kjeldsen, Marianne Kirstine; Bek, Dorte; Rasmussen, Erik Michael

    2009-01-01

    A line probe assay (GenoType MTBC) was evaluated for species differentiation within the Mycobacterium tuberculosis complex (MTBC). We included 387 MTBC isolates, 43 IS6110 low-copy MTBC isolates, 28 clinical specimens with varying microscopy grade, and 30 isolates of non-tuberculous mycobacteria...

  6. Upgrading transmission lines with aerial LiDAR technology

    Energy Technology Data Exchange (ETDEWEB)

    Koop, J.E. [Manitoba Hydro, Winnipeg, MB (Canada)

    2003-04-01

    LiDAR (Light Detection and Ranging) technology is described as an example of techniques used by hydro companies to increase their capacity with existing plants, and within tight budget constraints. LiDAR was chosen by Manitoba Hydro primarily because LiDAR's data collection method offers very fast turn-around time from collection to delivery, and most importantly because of LiDAR's highly accurate ability to map terrain and wire catenary shape in every span. The article describes a case study of the 'Nip and Tuck' method of wire re-tensioning based on LiDAR data, which was used by Manitoba Hydro to create a computer model of Saskatchewan Hydro's transmission line capacity on its 138 kV transmission line between Saskatoon and North Battleford. The model was needed to analyze the existing line conditions in an effort to minimize cascading failures on the 40-year old line. Using the 'Nip and Tuck' technology in combination with LiDAR, SaskPower engineers were able to complete the required modifications to raise transmission wire operating temperatures on the 135 km long line to 66 degree C in only 36 days, and at a cost that was 80 per cent less than the cost would have been using conventional techniques ($232,000 instead of the estimated $1.25 million).

  7. Diagnostic performance of automated liquid culture and molecular line probe assay in smear-negative pulmonary tuberculosis.

    Science.gov (United States)

    Kotwal, Aarti; Biswas, Debasis; Raghuvanshi, Shailendra; Sindhwani, Girish; Kakati, Barnali; Sharma, Shweta

    2017-04-01

    The diagnosis of smear-negative pulmonary tuberculosis (PTB) is particularly challenging, and automated liquid culture and molecular line probe assays (LPA) may prove particularly useful. The objective of our study was to evaluate the diagnostic potential of automated liquid culture (ALC) technology and commercial LPA in sputum smear-negative PTB suspects. Spot sputum samples were collected from 145 chest-symptomatic smear-negative patients and subjected to ALC, direct drug susceptibility test (DST) testing and LPA, as per manufacturers' instructions. A diagnostic yield of 26.2% was observed among sputum smear-negative TB suspects with 47.4% of the culture isolates being either INH- and/or rifampicin-resistant. Complete agreement was observed between the results of ALC assay and LPA except for two isolates which demonstrated sensitivity to INH and rifampicin at direct DST but were rifampicin-resistant in LPA. Two novel mutations were also detected among the multidrug isolates by LPA. In view of the diagnostic challenges associated with the diagnosis of TB in sputum smear-negative patients, our study demonstrates the applicability of ALC and LPA in establishing diagnostic evidence of TB.

  8. The application of micro-coil NMR probe technology to metabolomics of urine and serum

    International Nuclear Information System (INIS)

    Grimes, John H.; O’Connell, Thomas M.

    2011-01-01

    Increasing the sensitivity and throughput of NMR-based metabolomics is critical for the continued growth of this field. In this paper the application of micro-coil NMR probe technology was evaluated for this purpose. The most commonly used biofluids in metabolomics are urine and serum. In this study we examine different sample limited conditions and compare the detection sensitivity of the micro-coil with a standard 5 mm NMR probe. Sample concentration is evaluated as a means to leverage the greatly improved mass sensitivity of the micro-coil probes. With very small sample volumes, the sensitivity of the micro-coil probe does indeed provide a significant advantage over the standard probe. Concentrating the samples does improve the signal detection, but the benefits do not follow the expected linear increase and are both matrix and metabolite specific. Absolute quantitation will be affected by concentration, but an analysis of relative concentrations is still possible. The choice of the micro-coil probe over a standard tube based probe will depend upon a number of factors including number of samples and initial volume but this study demonstrates the feasibility of high-throughput metabolomics with the micro-probe platform.

  9. In-line optical fiber metallic mirror reflector for monolithic common path optical coherence tomography probes.

    Science.gov (United States)

    Singh, Kanwarpal; Reddy, Rohith; Sharma, Gargi; Verma, Yogesh; Gardecki, Joseph A; Tearney, Guillermo

    2018-03-01

    Endoscopic optical coherence tomography probes suffer from various artifacts due to dispersion imbalance and polarization mismatch between reference and sample arm light. Such artifacts can be minimized using a common path approach. In this work, we demonstrate a miniaturized common path probe for optical coherence tomography using an inline fiber mirror. A common path optical fiber probe suitable for performing high-resolution endoscopic optical coherence tomography imaging was developed. To achieve common path functionality, an inline fiber mirror was fabricated using a thin gold layer. A commercially available swept source engine was used to test the designed probe in a cadaver human coronary artery ex vivo. We achieved a sensitivity of 104 dB for this probe using a swept source optical coherence tomography system. To test the probe, images of a cadaver human coronary artery were obtained, demonstrating the quality that is comparable to those obtained by OCT systems with separate reference arms. Additionally, we demonstrate recovery of ranging depth by use of a Michelson interferometer in the detection path. We developed a miniaturized monolithic inline fiber mirror-based common path probe for optical coherence tomography. Owing to its simplicity, our design will be helpful in endoscopic applications that require high-resolution probes in a compact form factor while reducing system complexity. Lasers Surg. Med. 50:230-235, 2018. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  10. Design and demonstration of an intracortical probe technology with tunable modulus.

    Science.gov (United States)

    Simon, Dustin M; Charkhkar, Hamid; St John, Conan; Rajendran, Sakthi; Kang, Tong; Reit, Radu; Arreaga-Salas, David; McHail, Daniel G; Knaack, Gretchen L; Sloan, Andrew; Grasse, Dane; Dumas, Theodore C; Rennaker, Robert L; Pancrazio, Joseph J; Voit, Walter E

    2017-01-01

    Intracortical probe technology, consisting of arrays of microelectrodes, offers a means of recording the bioelectrical activity from neural tissue. A major limitation of existing intracortical probe technology pertains to limited lifetime of 6 months to a year of recording after implantation. A major contributor to device failure is widely believed to be the interfacial mechanical mismatch of conventional stiff intracortical devices and the surrounding brain tissue. We describe the design, development, and demonstration of a novel functional intracortical probe technology that has a tunable Young's modulus from ∼2 GPa to ∼50 MPa. This technology leverages advances in dynamically softening materials, specifically thiol-ene/acrylate thermoset polymers, which exhibit minimal swelling of memory polymer-based multichannel intracortical probe can be fabricated, that the mechanical properties are stable for at least 2 months and that the device is capable of single unit recordings for durations up to 77 days in vivo. This novel technology, which is amenable to processes suitable for manufacturing via standard semiconductor fabrication techniques, offers the capability of softening in vivo to reduce the tissue-device modulus mismatch to ultimately improve long term viability of neural recordings. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 159-168, 2017. © 2016 Wiley Periodicals, Inc.

  11. Linee di ricerca emergenti nell’educational technology

    Directory of Open Access Journals (Sweden)

    Maria Ranieri

    2015-12-01

    Full Text Available Il presente contributo intende restituire un quadro delle linee di ricerca emergenti nel campo dell’educational technology. Le fonti utilizzate a questo scopo e illustrate nella sezione introduttiva hanno permesso di individuare sei aree di interesse, vale a dire: apprendere in mobilità; social web e apprendimento; learning analytics; digital literacy/digital reading; OER e MOOC; ambienti immersivi e videogame. Per ciascuna di queste aree vengono delineate le principali direttrici di ricerca che le connotano e i relativi avanzamenti. Il lavoro si conclude con alcune considerazioni di sintesi sulle tendenze generali della ricerca nel settore.

  12. Goal line technology in soccer: Are referees ready for technology in ...

    African Journals Online (AJOL)

    When controversial decisions are made, the referee comes under extensive scrutiny by players, fans and spectators as well as commentators who have the tendency of scapegoating the referee. The purpose of the study was to examine the perceptions of soccer referees regarding the use of goal line technology in soccer.

  13. Portable reconfigurable line sensor (PRLS) and technology transfer

    International Nuclear Information System (INIS)

    MacKenzie, D.P.; Buckle, T.H.; Blattman, D.A.

    1993-01-01

    The Portable Reconfigurable Line Sensor (PRLS) is a bistatic, pulsed-Doppler, microwave intrusion detection system developed at Sandia National Laboratories for the US Air Force. The PRLS is rapidly and easily deployed, and can detect intruders ranging from a slow creeping intruder to a high speed vehicle. The system has a sharply defined detection zone and will not falsely alarm on nearby traffic. Unlike most microwave sensors, the PRLS requires no alignment or calibration. Its portability, battery operation, ease of setup, and RF alarm reporting capability make it an excellent choice for perimeter, portal, and gap-filler applications in the important new field of rapidly-deployable sensor systems. In October 1992, the US Air Force and Racon, Inc., entered into a Cooperative Research and Development Agreement (CRADA) to commercialize the PRLS, jointly sharing government and industry resources. The Air Force brings the user's perspective and requirements to the cooperative effort. Sandia, serving as the technical arm of the Air Force, adds the actual PRLS technology to the joint effort, and provides security systems and radar development expertise. Racon puts the Air Force requirements and Sandia technology together into a commercial product, making the system meet important commercial manufacturing constraints. The result is a true ''win-win'' situation, with reduced government investment during the commercial development of the PRLS, and industry access to technology not otherwise available

  14. Designing assisted living technologies ‘in the wild’: preliminary experiences with cultural probe methodology

    Directory of Open Access Journals (Sweden)

    Wherton Joseph

    2012-12-01

    Full Text Available Abstract Background There is growing interest in assisted living technologies to support independence at home. Such technologies should ideally be designed ‘in the wild’ i.e. taking account of how real people live in real homes and communities. The ATHENE (Assistive Technologies for Healthy Living in Elders: Needs Assessment by Ethnography project seeks to illuminate the living needs of older people and facilitate the co-production with older people of technologies and services. This paper describes the development of a cultural probe tool produced as part of the ATHENE project and how it was used to support home visit interviews with elders with a range of ethnic and social backgrounds, family circumstances, health conditions and assisted living needs. Method Thirty one people aged 60 to 98 were visited in their homes on three occasions. Following an initial interview, participants were given a set of cultural probe materials, including a digital camera and the ‘Home and Life Scrapbook’ to complete in their own time for one week. Activities within the Home and Life Scrapbook included maps (indicating their relationships to people, places and objects, lists (e.g. likes, dislikes, things they were concerned about, things they were comfortable with, wishes (things they wanted to change or improve, body outline (indicating symptoms or impairments, home plan (room layouts of their homes to indicate spaces and objects used and a diary. After one week, the researcher and participant reviewed any digital photos taken and the content of the Home and Life Scrapbook as part of the home visit interview. Findings The cultural probe facilitated collection of visual, narrative and material data by older people, and appeared to generate high levels of engagement from some participants. However, others used the probe minimally or not at all for various reasons including limited literacy, physical problems (e.g. holding a pen, lack of time or energy

  15. Electromagnetically induced transparency line shapes for large probe fields and optically thick media

    International Nuclear Information System (INIS)

    Pack, M. V.; Camacho, R. M.; Howell, J. C.

    2007-01-01

    We calculate the line shape and linewidths for electromagnetically induced transparency (EIT) in optically thick, Doppler broadened media (buffer gasses are also considered). In generalizing the definition of the EIT linewidth to optically thick media, we find two different linewidth definitions apply depending on whether the experiment is pulsed or continuous wave (cw). Using the cw definition for the EIT line shape we derive analytic expressions describing the linewidth as a function of optical depth. We also review the EIT line shapes in optically thin media and provide physical arguments for how the line shapes change as a function of various parameters

  16. Agile machining and inspection thrust area team-on-machine probing / compatibility assessment of Parametric Technology Corporation (PTC) pro/CMM DMIS with Zeiss DMISEngine.

    Energy Technology Data Exchange (ETDEWEB)

    Wade, James Rokwel; Tomlinson, Kurt; Bryce, Edwin Anthony

    2008-09-01

    The charter goal of the Agile Machining and Inspection Thrust Area Team is to identify technical requirements, within the nuclear weapons complex (NWC), for Agile Machining and Inspection capabilities. During FY 2008, the team identified Parametric Technology Corporation (PTC) Pro/CMM as a software tool for use in off-line programming of probing routines--used for measurement--for machining and turning centers. The probing routine would be used for in-process verification of part geometry. The same Pro/CMM program used on the machine tool could also be employed for program validation / part verification using a coordinate measuring machine (CMM). Funding was provided to determine the compatibility of the Pro/CMM probing program with CMM software (Zeiss DMISEngine).

  17. Reverse line blot probe design and polymerase chain reaction optimization for bloodmeal analysis of ticks from the eastern United States.

    Science.gov (United States)

    Scott, M C; Harmon, J R; Tsao, J I; Jones, C J; Hickling, G J

    2012-05-01

    Determining the host preference of vector ticks is vital to elucidating the eco-epidemiology of the diseases they spread. Detachment of ticks from captured hosts can provide evidence of feeding on those host species, but only for those species that are feasible to capture. Recently developed, highly sensitive molecular assays show great promise in allowing host selection to be determined from minute traces of host DNA that persist in recently molted ticks. Using methods developed in Europe as a starting-point, we designed 12S rDNA mitochondrial gene probes suitable for use in a reverse line blot (RLB) assay of ticks feeding on common host species in the eastern United States. This is the first study to use the 12S mitochondrial gene in a RLB bloodmeal assay in North America. The assay combines conventional PCR with a biotin-labeled primer and reverse line blots that can be stripped and rehybridized up to 20 times, making the method less expensive and more straightforward to interpret than previous methods of tick bloodmeal identification. Probes were designed that target the species, genus, genus group, family, order, or class of eight reptile, 13 birds, and 32 mammal hosts. After optimization, the RLB assay correctly identified the current hostspecies for 99% of ticks [Amblyomma americanum (L.) and eight other ixodid tick species] collected directly from known hosts. The method identified previous-host DNA for approximately half of all questing ticks assayed. Multiple bloodmeal determinations were obtained in some instances from feeding and questing ticks; this pattern is consistent with previous RLB studies but requires further investigation. Development of this probe library, suitable for eastern U.S. ecosystems, opens new avenues for eco-epidemiological investigations of this region's tick-host systems.

  18. PROBING THE SOLAR ATMOSPHERE USING OSCILLATIONS OF INFRARED CO SPECTRAL LINES

    International Nuclear Information System (INIS)

    Penn, M. J.; Schad, T.; Cox, E.

    2011-01-01

    Oscillations were observed across the whole solar disk using the Doppler shift and line center intensity of spectral lines from the CO molecule near 4666 nm with the National Solar Observatory's McMath/Pierce solar telescope. Power, coherence, and phase spectra were examined, and diagnostic diagrams reveal power ridges at the solar global mode frequencies to show that these oscillations are solar p-modes. The phase was used to determine the height of formation of the CO lines by comparison with the IR continuum intensity phase shifts as measured in Kopp et al.; we find that the CO line formation height varies from 425 km μ > 0.5. The velocity power spectra show that while the sum of the background and p-mode power increases with height in the solar atmosphere as seen in previous work, the power in the p-modes only (background subtracted) decreases with height. The CO line center intensity weakens in regions of stronger magnetic fields, as does the p-mode oscillation power. Across most of the solar surface the phase shift is larger than the expected value of 90 0 for an adiabatic atmosphere. We fit the phase spectra at different disk positions with a simple atmospheric model to determine that the acoustic cutoff frequency is about 4.5 mHz with only small variations, but that the thermal relaxation frequency drops significantly from 2.7 to 0 mHz at these heights in the solar atmosphere.

  19. Probing the O-glycoproteome of Gastric Cancer Cell Lines for Biomarker Discovery

    DEFF Research Database (Denmark)

    Vieira Campos, Diana Alexandra; Freitas, Daniela; Gomes, Joana

    2015-01-01

    biomarker assays. However, the current knowledge of secreted and circulating O-glycoproteins is limited. Here, we used the COSMC KO "SimpleCell" (SC) strategy to characterize the O-glycoproteome of two gastric cancer SC lines (AGS, MKN45) as well as a gastric cell line (KATO III) which naturally expresses...... at least partially truncated O-glycans. Overall we identified 499 O-glycoproteins and 1,236 O-glycosites in gastric cancer SCs, and a total 47 O-glycoproteins and 73 O-glycosites in the KATO III cell line. We next modified the glycoproteomic strategy to apply it to pools of sera from gastric cancer...... with the STn glycoform were further validated as being expressed in gastric cancer tissue. A proximity ligation assay was used to demonstrate that CD44 was expressed with the STn glycoform in gastric cancer tissues. The study provides a discovery strategy for aberrantly glycosylated O-glycoproteins and a set...

  20. Fe-K LINE PROBING OF MATERIAL AROUND THE ACTIVE GALACTIC NUCLEUS CENTRAL ENGINE WITH SUZAKU

    International Nuclear Information System (INIS)

    Fukazawa, Yasushi; Hiragi, Kazuyoshi; Mizuno, Motohiro; Nishino, Sho; Hayashi, Katsuhiro; Yamasaki, Tomonori; Shirai, Hirohisa; Takahashi, Hiromitsu; Ohno, Masanori

    2011-01-01

    We systematically analyzed the high-quality Suzaku data of 88 Seyfert galaxies, about 31% of which are Compton-thick active galactic nuclei (AGNs). We obtained a clear relation between the absorption column density and the equivalent width (EW) of the 6.4 keV line above 10 23 cm -2 , suggesting a wide-ranging column density of 10 23 -10 24.5 cm -2 with a similar solid and an Fe abundance of 0.7-1.3 solar for Seyfert 2 galaxies. The EWs of the 6.4 keV line for Seyfert 1 galaxies are typically 40-120 eV, suggesting the existence of Compton-thick matter like the torus with a column density of >10 23 cm -2 and a solid angle of (0.15-0.4) x 4π, and no difference of neutral matter is visible between Seyfert 1 and 2 galaxies. An absorber with a lower column density of 10 21 -10 23 cm -2 for Compton-thin Seyfert 2 galaxies is suggested to be not a torus but an interstellar medium. These constraints can be understood by the fact that the 6.4 keV line intensity ratio against the 10-50 keV flux is almost identical within a range of 2-3 in many Seyfert galaxies. Interestingly, objects exist with a low EW, 10-30 eV, of the 6.4 keV line, suggesting that those torus subtends only a small solid angle of H >10 23 cm -2 indicates that the column density of the ionized material also increases together with that of the cold material. It is found that these features seem to change for brighter objects with more than several 10 44 erg s -1 such that the Fe-K line features become weak. This extends the previously known X-ray Baldwin effect on the neutral Fe-Kα line to ionized emission or absorption lines. The luminosity dependence of these properties, regardless of the scatter of black hole mass by two orders of magnitudes, indicates that the ionized material is associated with the structure of the parent galaxy rather than the outflow from the nucleus.

  1. PROBING THE ROLE OF CARBON IN ULTRAVIOLET EXTINCTION ALONG GALACTIC SIGHT LINES

    Energy Technology Data Exchange (ETDEWEB)

    Parvathi, V. S.; Babu, B. R. S. [Department of Physics, University of Calicut, Kerala 673635 (India); Sofia, U. J. [Department of Physics, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016 (United States); Murthy, J., E-mail: veena.makesh@gmail.com, E-mail: brsbabu@gmail.com, E-mail: sofia@american.edu, E-mail: jmurthy@yahoo.com [Indian Institute of Astrophysics, II Block, Koramangala, Bangalore 560034 (India)

    2012-11-20

    We report previously undetermined interstellar gas and dust-phase carbon abundances along 15 Galactic sight lines based on archival data of the strong 1334.5323 A transition observed with the Space Telescope Imaging Spectrograph. These are combined with previously reported carbon measurements along six sight lines to produce a complete sample of interstellar C II measurements determined with the 1334 A transition. Our data set includes a variety of Galactic disk environments characterized by different extinctions and samples paths ranging over three orders of magnitude in average density of hydrogen ((n(H))). Our data support the idea that dust, specifically carbon-based grains, are processed in the neutral interstellar medium. We, however, do not find that the abundance of carbon in dust or the grain-size distribution is related to the strength of the 2175 A bump. This is surprising, given that many current models have polycyclic aromatic hydrocarbons as the bump-producing dust.

  2. PROBING THE FLARE ATMOSPHERES OF M DWARFS USING INFRARED EMISSION LINES

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, Sarah J.; Kowalski, Adam F.; Hawley, Suzanne L.; Hilton, Eric J.; Wisniewski, John P.; Tofflemire, Benjamin M., E-mail: sjschmidt@astro.washington.edu [Dominion Astrophysical Observatory, Herzberg Institute of Astrophysics, National Research Council of Canada (Canada)

    2012-01-20

    We present the results of a campaign to monitor active M dwarfs using infrared spectroscopy, supplemented with optical photometry and spectroscopy. We detected 16 flares during nearly 50 hr of observations on EV Lac, AD Leo, YZ CMi, and VB 8. The three most energetic flares also showed infrared emission, including the first reported detections of P{beta}, P{gamma}, He I {lambda}10830, and Br{gamma} during an M dwarf flare. The strongest flare ({Delta}u = 4.02 on EV Lac) showed emission from H{gamma}, H{delta}, He I {lambda}4471, and Ca II K in the UV/blue and P{beta}, P{gamma}, P{delta}, Br{gamma}, and He I {lambda}10830 in the infrared. The weaker flares ({Delta}u = 1.68 on EV Lac and {Delta}U = 1.38 on YZ CMi) were only observed with photometry and infrared spectroscopy; both showed emission from P{beta}, P{gamma}, and He I {lambda}10830. The strongest infrared emission line, P{beta}, occurred in the active mid-M dwarfs with a duty cycle of {approx}3%-4%. To examine the most energetic flare, we used the static NLTE radiative transfer code RH to produce model spectra based on a suite of one-dimensional model atmospheres. Using a hotter chromosphere than previous one-dimensional atmospheric models, we obtain line ratios that match most of the observed emission lines.

  3. On-line probe for fast electrochemistry/electrospray mass spectrometry. Investigation of polycyclic aromatic hydrocarbons.

    Science.gov (United States)

    Xu, X; Lu, W; Cole, R B

    1996-12-01

    A newly invented probe accessory for fast electrochemistry/electrospray mass spectrometry (EC/ESMS) is presented and evaluated. The device features a low-volume, three-electrode electrochemical cell which has been designed with a minimum distance between the working electrode and the "Taylor cone" inherent to the electrospray process. This configuration limits the time between electrochemical generation of ions and mass spectrometric analysis to an absolute minimum. A fused-silica layer insulates the microcylinder working electrode from the sample solution until immediately prior to the electrospray region, postponing electrode processes until the last moment. The same fused-silica layer insulates the working electrode from the surrounding auxiliary electrode, a stainless steel capillary that also serves as the electrospray capillary. The performance and capabilities of the novel electrochemistry/electrospray mass spectrometry system have been evaluated using polycyclic aromatic hydrocarbons (PAHs) as test analytes. In the positive ion EC/ESMS mode, oxidized forms (one-electron removal) of PAHs are produced in high yield. The ability to analyze reaction products appearing subsequent to the initial oxidation is also demonstrated.

  4. Probing the water and CO snow lines in the young protostar NGC 1333-IRAS4B

    Science.gov (United States)

    Anderl, Sibylle; Maret, Sébastien; André, Philippe; Maury, Anaëlle; Belloche, Arnaud; Cabrit, Sylvie; Codella, Claudio; Lefloch, Bertrand

    2015-08-01

    Today, we believe that the onset of life requires free energy, water, and complex, probably carbon-based chemistry. In the interstellar medium, complex organic molecules seem to mostly form in reactions happening on the icy surface of dust grains, such that they are released into the gas phase when the dust is heated. The resulting “snow lines”, marking regions where ices start to sublimate, play an important role for planet growth and bulk composition in protoplanetary disks. However, they can already be observed in the envelopes of the much younger, low-mass Class 0 protostars that are still in their early phase of heavy accretion. The information on the sublimation regions of different kinds of ices can be used to understand the chemistry of the envelope, its temperature and density structure, and may even hint at the history of the accretion process. Accordingly, it is a crucial piece of information in order to get the full picture of how organic chemistry evolves already at the earliest stages of the formation of sun-like stars. As part of the CALYPSO Large Program (http://irfu.cea.fr/Projets/Calypso/), we have obtained observations of C18O, N2H+ and CH3OH towards the Class 0 protostar NGC 1333-IRAS4B with the IRAM Plateau de Bure interferometer at sub-arcsecond resolution. Of these we use the methanol observations as a proxy for the water snow line, assuming methanol is trapped in water ice. The observed anti-correlation of C18O and N2H+, with N2H+ forming a ring around the centrally peaked C18O emission, reveals for the first time the CO snow line in this protostellar envelope, with a radius of ~300 AU. The methanol emission is much more compact than that of C18O, and traces the water snow line with a radius of ~40 AU. We have modeled the emission using a chemical model coupled with a radiative transfer module. We find that the CO snow line appears further inwards than expected from the binding energy of pure CO ices. This may hint at CO being frozen out

  5. A COMPARISON OF DNA DAMAGE PROBES IN TWO HMEC LINES WITH X-IRRADIATION

    Energy Technology Data Exchange (ETDEWEB)

    Wisnewski, C.L.; Bjornstad, K.A.; Rosen, C.J.; Chang, P.Y.; Blakely, E.A.

    2007-01-01

    In this study, we investigated γH2AXser139 and 53BP1ser25, DNA damage pathway markers, to observe responses to radiation insult. Two Human Mammary Epithelial Cell (HMEC) lines were utilized to research the role of immortalization in DNA damage marker expression, HMEC HMT-3522 (S1) with an infi nite lifespan, and a subtype of HMEC 184 (184V) with a fi nite lifespan. Cells were irradiated with 50cGy X-rays, fi xed with 4% paraformaldehyde after 1 hour repair at 37°C, and processed through immunofl uorescence. Cells were visualized with a fl uorescent microscope and images were digitally captured using Image-Pro Plus software. The 184V irradiated cells exhibited a more positive punctate response within the nucleus for both DNA damage markers compared to the S1 irradiated cells. The dose and time course will be expanded in future studies to augment the preliminary data from this research. It is important to understand whether the process of transformation to immortalization compromises the DNA damage sensor and repair process proteins of HMECs in order to understand what is “normal” and to evaluate the usefulness of cell lines as experimental models.

  6. A comparison of DNA damage probes in two HMEC lines withX-irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Wisnewski, Christy L.; Bjornstad, Kathleen A.; Rosen, ChristoperJ.; Chang, Polly Y.; Blakely, Eleanor A.

    2007-01-19

    In this study, we investigated {gamma}H2AX{sup ser139} and 53BP1{sup ser25}, DNA damage pathway markers, to observe responses to radiation insult. Two Human Mammary Epithelial Cell (HMEC) lines were utilized to research the role of immortalization in DNA damage marker expression, HMEC HMT-3522 (S1) with an infinite lifespan, and a subtype of HMEC 184 (184V) with a finite lifespan. Cells were irradiated with 50 cGy X-rays, fixed with 4% paraformaldehyde after 1 hour repair at 37 C, and processed through immunofluorescence. Cells were visualized with a fluorescent microscope and images were digitally captured using Image-Pro Plus software. The 184V irradiated cells exhibited a more positive punctate response within the nucleus for both DNA damage markers compared to the S1 irradiated cells. We will expand the dose and time course in future studies to augment the preliminary data from this research. It is important to understand whether the process of transformation to immortalization compromises the DNA damage sensor and repair process proteins of HMECs in order to understand what is 'normal' and to evaluate the usefulness of cell lines as experimental models.

  7. Micro- and nano-technologies to probe the mechano-biology of the brain.

    Science.gov (United States)

    Tay, Andy; Schweizer, Felix E; Di Carlo, Dino

    2016-05-24

    Biomechanical forces have been demonstrated to influence a plethora of neuronal functions across scales including gene expression, mechano-sensitive ion channels, neurite outgrowth and folding of the cortices in the brain. However, the detailed roles biomechanical forces may play in brain development and disorders has seen limited study, partly due to a lack of effective methods to probe the mechano-biology of the brain. Current techniques to apply biomechanical forces on neurons often suffer from low throughput and poor spatiotemporal resolution. On the other hand, newly developed micro- and nano-technologies can overcome these aforementioned limitations and offer advantages such as lower cost and possibility of non-invasive control of neuronal circuits. This review compares the range of conventional, micro- and nano-technological techniques that have been developed and how they have been or can be used to understand the effect of biomechanical forces on neuronal development and homeostasis.

  8. 1-Deoxynojirimycins with dansyl capped N-substituents as probes for Morbus Gaucher affected cell lines.

    Science.gov (United States)

    Fröhlich, Richard F G; Furneaux, Richard H; Mahuran, Don J; Rigat, Brigitte A; Stütz, Arnold E; Tropak, Michael B; Wicki, Jacqueline; Withers, Stephen G; Wrodnigg, Tanja M

    2010-07-02

    Cyclization by double reductive amination of d-xylo-hexos-5-ulose with methyl 6-aminohexanoate gave (methoxycarbonyl)pentyl-1-deoxynojirimycin. Reaction of the terminal carboxylic acid with N-dansyl-1,6-diaminohexane provided the corresponding chain-extended fluorescent derivative. By reaction with bis(6-dansylaminohexyl)amine, the corresponding branched di-N-dansyl compound was obtained. Both compounds are strong inhibitors of d-glucosidases and could also be shown to distinctly improve, at sub-inhibitory concentrations, the activity of beta-glucocerebrosidase in a Gaucher fibroblast (N370S) cell-line through chaperoning of the enzyme to the lysosome. Copyright 2010 Elsevier Ltd. All rights reserved.

  9. Dynamic Line Rating - Technologies and Challenges of PMU on Overhead Lines

    DEFF Research Database (Denmark)

    Alvarez, David; Rosero, Javier; Silva, Filipe Miguel Faria da

    2016-01-01

    for line rating computation and monitoring are identified, these are: sensors, communications, management information system and information analysis tools, which are part of integral dynamic line rating systems. Finally, the benefits and challenges of using phasor measurement units for real time capacity...

  10. MULTI-WAVELENGTH PROBES OF OBSCURATION TOWARD THE NARROW-LINE REGION IN SEYFERT GALAXIES

    International Nuclear Information System (INIS)

    Kraemer, S. B.; Schmitt, H.R.; Crenshaw, D. M.; Melendez, M.; Turner, T.J.; Guainazzi, M.; Mushotzky, R.F.

    2011-01-01

    We present a study of reddening and absorption toward the narrow line regions (NLRs) in active galactic nuclei (AGNs) selected from the Revised Shapley-Ames, 12 μm, and Swift/Burst Alert Telescope samples. For the sources in host galaxies with inclinations of b/a > 0.5, we find that the mean ratio of [O III] λ5007, from ground-based observations, and [O IV] 28.59 μm, from Spitzer/Infrared Spectrograph observations, is a factor of two lower in Seyfert 2s than Seyfert 1s. The combination of low [O III]/[O IV] and [O III] λ4363/λ5007 ratios in Seyfert 2s suggests more extinction of emission from the NLR than in Seyfert 1s. Similar column densities of dusty gas, N H ∼ several x 10 21 cm -2 , can account for the suppression of both [O III] λ5007 and [O III] λ4363, as compared to those observed in Seyfert 1s. Also, we find that the X-ray line O VII λ22.1 A is weaker in Seyfert 2s, consistent with absorption by the same gas that reddens the optical emission. Using a Hubble Space Telescope/Space Telescope Imaging Spectrograph slitless spectrum of the Seyfert 1 galaxy NGC 4151, we estimate that only ∼30% of the [O III] λ5007 comes from within 30 pc of the central source, which is insufficient to account for the low [O III]/[O IV] ratios in Seyfert 2s. If Seyfert 2 galaxies have similar intrinsic [O III] spatial profiles, the external dusty gas must extend further out along the NLR, perhaps in the form of nuclear dust spirals that have been associated with fueling flows toward the AGN.

  11. Signal amplification of microRNAs with modified strand displacement-based cycling probe technology.

    Science.gov (United States)

    Jia, Huning; Bu, Ying; Zou, Bingjie; Wang, Jianping; Kumar, Shalen; Pitman, Janet L; Zhou, Guohua; Song, Qinxin

    2016-10-24

    Micro ribose nucleic acids (miRNAs) play an important role in biological processes such as cell differentiation, proliferation and apoptosis. Therefore, miRNAs are potentially a powerful marker for monitoring cancer and diagnosis. Here, we present sensitive signal amplification for miRNAs based on modified cycling probe technology with strand displacement amplification. miRNA was captured by the template coupled with beads, and then the first cycle based on SDA was repeatedly extended to the nicking end, which was produced by the extension reaction of miRNA. The products generated by SDA are captured by a molecular beacon (MB), which is designed to initiate the second amplification cycle, with a similar principle to the cycling probe technology (CPT), which is based on repeated digestion of the DNA-RNA hybrid by the RNase H. After one sample enrichment and two steps of signal amplification, 0.1 pM of let-7a can be detected. The miRNA assay exhibits a great dynamic range of over 100 orders of magnitude and high specificity to clearly discriminate a single base difference in miRNA sequences. This isothermal amplification does not require any special temperature control instrument. The assay is also about signal amplification rather than template amplification, therefore minimising contamination issues. In addition, there is no need for the reverse transcription (RT) process. Thus the amplification is suitable for miRNA detection.

  12. High precision micro-scale Hall Effect characterization method using in-line micro four-point probes

    DEFF Research Database (Denmark)

    Petersen, Dirch Hjorth; Hansen, Ole; Lin, Rong

    2008-01-01

    Accurate characterization of ultra shallow junctions (USJ) is important in order to understand the principles of junction formation and to develop the appropriate implant and annealing technologies. We investigate the capabilities of a new micro-scale Hall effect measurement method where Hall...... effect is measured with collinear micro four-point probes (M4PP). We derive the sensitivity to electrode position errors and describe a position error suppression method to enable rapid reliable Hall effect measurements with just two measurement points. We show with both Monte Carlo simulations...... and experimental measurements, that the repeatability of a micro-scale Hall effect measurement is better than 1 %. We demonstrate the ability to spatially resolve Hall effect on micro-scale by characterization of an USJ with a single laser stripe anneal. The micro sheet resistance variations resulting from...

  13. Research on UAV Intelligent Obstacle Avoidance Technology During Inspection of Transmission Line

    Science.gov (United States)

    Wei, Chuanhu; Zhang, Fei; Yin, Chaoyuan; Liu, Yue; Liu, Liang; Li, Zongyu; Wang, Wanguo

    Autonomous obstacle avoidance of unmanned aerial vehicle (hereinafter referred to as UAV) in electric power line inspection process has important significance for operation safety and economy for UAV intelligent inspection system of transmission line as main content of UAV intelligent inspection system on transmission line. In the paper, principles of UAV inspection obstacle avoidance technology of transmission line are introduced. UAV inspection obstacle avoidance technology based on particle swarm global optimization algorithm is proposed after common obstacle avoidance technologies are studied. Stimulation comparison is implemented with traditional UAV inspection obstacle avoidance technology which adopts artificial potential field method. Results show that UAV inspection strategy of particle swarm optimization algorithm, adopted in the paper, is prominently better than UAV inspection strategy of artificial potential field method in the aspects of obstacle avoidance effect and the ability of returning to preset inspection track after passing through the obstacle. An effective method is provided for UAV inspection obstacle avoidance of transmission line.

  14. Technology, Pedagogy and On line materials: Keywords in developing On line Education

    DEFF Research Database (Denmark)

    Georgsen, Marianne; Bennedsen, Jens

    2003-01-01

    , meaning that technology itself and the use of technology are are interrelated relationship between technology and the use of technology s a dialectic are culturally and contextually situated activities. Furthermore, the and teaching is characterized by the fact that learning and teaching The development...... of ICT-based learning and are presented. Two empirical cases are described, and lessons learned from the project .(learned in a pedagogical and organisational development project (Flexnet teaching and learning. The model is developed on the basis of lessons presents a model for planning and developing...... flexible ICT-supported and reflective and critical thinking about use of technology. This paper and learning, development must be based on systematic pedagogical reflections a high degree of flexibility in online education....

  15. TSA's Mulligan clarified line between technology, First Amendment

    OpenAIRE

    Center for Homeland Defense and Security

    2010-01-01

    Center for Homeland Defense and Security, OUT OF THE CLASSROOM Download the paper: Technology for Homeland Security Course Paper” In the summer of 2007, Indiana State University student Christopher Soghoian posted an item on his internet website, Chris’ Northwest Airlines...

  16. Seniors-on-line: introducing older people to technology.

    Science.gov (United States)

    Irizarry, C; Downing, A; Elford, C

    1997-03-01

    Retired Engineers are playing an important role in ensuring that older people are not excluded from the benefits of technological advances. Technology is playing an increasingly important role in the lives of older people as it is incorporated into assistive devices, home security, access to health care, banking, communication and many other areas. However, if older people are unfamiliar with new technologies and find them daunting, they may not benefit fully from these advances. In order to minimize difficulties arising from unfamiliarity with technology, an introductory computer course was offered to people aged 55 and over. Teaching methods appropriate to the needs of older people were used: small classes, students and instructors from same age cohort, slow pace of presentation and ample opportunity to ask questions. Retired Engineers make up the majority of instructors. Three hundred and sixty nine older people have participated in the course and most plan to continue using a computer.

  17. Evaluation of a polymerase chain reaction reverse hybridization line probe assay for the detection and identification of medically important fungi in bronchoalveolar lavage fluids.

    NARCIS (Netherlands)

    Meletiadis, J.; Melchers, W.J.G.; Meis, J.F.G.M.; Hurk, P.J.J.C. van den; Jannes, G.; Verweij, P.E.

    2003-01-01

    An assay system in which polymerase chain reaction (PCR) amplification of the ITS-1 region of ribosomal DNA (rDNA) is combined with a reverse-hybridization line probe assay (LiPA) was used for the identification of six Candida species and four Aspergillus species in pure cultures of clinical

  18. On-line radiation teaching materials using IT technology

    International Nuclear Information System (INIS)

    Inoue, Hiroyoshi

    2005-01-01

    We developed the on-line radiation teaching materials using the Internet, in order to provide the teaching support materials of atomic power and radiation educations in on-school study, as well as to create the complementary study system in off-school study. The themes of teaching materials were selected from requests by teachers. In the case of an elementary school, the teaching material 'an environmental problem and atomic power' was created as the aggregate of each content for study without boundary between subjects. The teaching material 'medical treatment and radiation' was created for junior high school students to raise the individual knowledge. In the case of a high school, the teaching material nucleus and radiation' was prepared to supplement the physical study of students. The on-line teaching materials were tried to 300 junior high school and high school students, 68% of students answered that the teaching material is effective to understand atomic power and radiation, though 17% answered they were not effective. Although there are problems to prepare IT learning equipments and learning follow-up system in the material, it is suggested that the on-line teaching materials will provide the novel learning system including debates for the study. This method has no limitation of time and place. (author)

  19. Conductance measurement by two-line probe method of polypyrrole nano-films formed on mica by admicellar polymerization

    Energy Technology Data Exchange (ETDEWEB)

    Mou, C.-Y. [Graduate Institute of Textile Engineering, Feng Chia University, Taichung 40724, Taiwan (China); Yuan, W.-L. [Department of Chemical Engineering, Feng Chia University, Taichung 40724, Taiwan (China)], E-mail: wyuan@fcu.edu.tw; Tsai, I-S. [Graduate Institute of Textile Engineering, Feng Chia University, Taichung 40724, Taiwan (China); O' Rear, Edgar A. [School of Chemical, Biological and Material Engineering, University of Oklahoma, Norman, OK 73019 (United States); Barraza, Harry [Unilever R and D HPC, Quarry Road East, Bebington, Wirral, CH63 3JW (United Kingdom)

    2008-10-01

    Measuring the electrical conductance is of importance in fabricating electronic devices based on semiconducting thin films. In this report, electrically conducting polypyrrole (PPy) nano-films were deposited on insulating mica plates by admicellar polymerization. It becomes difficult to measure such film conductance in the lateral direction due the nanometric thickness which only allows for very low electrical current. In order to understand the effects of surfactant on the film conductivity, morphological studies using atomic force microscopy and conductance measurements with a sub-fA multimeter were performed. Higher conductances were found for PPy thin films made using surfactant templates, than that of a bare mica surface. Using the two-line probe method by drawing two lines of silver glue 8 mm apart on the sample surface, the current-voltage curves of bare mica surface yielded a lateral conductance of 6.0 x 10{sup -13} S. In comparison, PPy thin films made using sodium dodecyl sulfate (SDS) and cetyl trimethyl ammonium bromide (CTAB) as surfactant templates showed conductances of 1.2 x 10{sup -11} S and 7.7 x 10{sup -12} S, respectively. The higher conductances indicate tunneling, hopping, and percolation of charge carriers throughout the films. The lower-bound conductivities were calculated as 4.0 x 10{sup -3} S/cm and 2.6 x 10{sup -3} S/cm, measured based on the average thickness 2.3 nm for the SDS-PPy films and 2.4 nm for the CTAB-PPy films. Conductivities for both SDS and CTAB template PPy films are found to be of the same order.

  20. Conductance measurement by two-line probe method of polypyrrole nano-films formed on mica by admicellar polymerization

    International Nuclear Information System (INIS)

    Mou, C.-Y.; Yuan, W.-L.; Tsai, I-S.; O'Rear, Edgar A.; Barraza, Harry

    2008-01-01

    Measuring the electrical conductance is of importance in fabricating electronic devices based on semiconducting thin films. In this report, electrically conducting polypyrrole (PPy) nano-films were deposited on insulating mica plates by admicellar polymerization. It becomes difficult to measure such film conductance in the lateral direction due the nanometric thickness which only allows for very low electrical current. In order to understand the effects of surfactant on the film conductivity, morphological studies using atomic force microscopy and conductance measurements with a sub-fA multimeter were performed. Higher conductances were found for PPy thin films made using surfactant templates, than that of a bare mica surface. Using the two-line probe method by drawing two lines of silver glue 8 mm apart on the sample surface, the current-voltage curves of bare mica surface yielded a lateral conductance of 6.0 x 10 -13 S. In comparison, PPy thin films made using sodium dodecyl sulfate (SDS) and cetyl trimethyl ammonium bromide (CTAB) as surfactant templates showed conductances of 1.2 x 10 -11 S and 7.7 x 10 -12 S, respectively. The higher conductances indicate tunneling, hopping, and percolation of charge carriers throughout the films. The lower-bound conductivities were calculated as 4.0 x 10 -3 S/cm and 2.6 x 10 -3 S/cm, measured based on the average thickness 2.3 nm for the SDS-PPy films and 2.4 nm for the CTAB-PPy films. Conductivities for both SDS and CTAB template PPy films are found to be of the same order

  1. Blurred Lines: The School Librarian and the Instructional Technology Specialist

    Science.gov (United States)

    Johnston, Melissa P.

    2015-01-01

    "Empowering Learners: Guidelines for School Library Media Programs" (AASL, 2009) charges school librarians "to play a leading role in weaving such skills throughout the curriculum so that all members of the school community are effective users of ideas and information" (p. 46). Providing leadership in technology integration for…

  2. 2011 annual meeting on nuclear technology fully on line

    International Nuclear Information System (INIS)

    Anon.

    2010-01-01

    The 2010 ANNUAL MEETING ON NUCLEAR TECHNOLOGY, in its familiar structure of 3 days of conferencing about topics from politics, economy, and technology, was the forum for presentations and discussions in the field of nuclear power. Participants accepted the new concept. This was borne out in particular by the great interest shown in the pre-conference evening with its keynote address, but also by the success of the plenary day, which included a press forum and a panel discussion as components of active communication making the plenary day much more attractive. The 2011 Annual Meeting on Nuclear Technology will be held again at the Berlin Congress Center (bcc) in Alexanderplatz on May 17-19. From September 1, some first important information is available under www.kerntechnik.info, for instance, the call for Papers. All other information about the program will be published in due course. All steps of importance to participants, from registration for the meeting to booking hotel accommodation, can be handled online. (orig.)

  3. Structural health monitoring of pipelines rehabilitated with lining technology

    Science.gov (United States)

    Farhidzadeh, Alireza; Dehghan-Niri, Ehsan; Salamone, Salvatore

    2014-03-01

    Damage detection of pipeline systems is a tedious and time consuming job due to digging requirement, accessibility, interference with other facilities, and being extremely wide spread in metropolitans. Therefore, a real-time and automated monitoring system can pervasively reduce labor work, time, and expenditures. This paper presents the results of an experimental study aimed at monitoring the performance of full scale pipe lining systems, subjected to static and dynamic (seismic) loading, using Acoustic Emission (AE) technique and Guided Ultrasonic Waves (GUWs). Particularly, two damage mechanisms are investigated: 1) delamination between pipeline and liner as the early indicator of damage, and 2) onset of nonlinearity and incipient failure of the liner as critical damage state.

  4. Synthesis of dansyl-labeled probe of thiophene analogue of annonaceous acetogenins for visualization of cell distribution and growth inhibitory activity toward human cancer cell lines.

    Science.gov (United States)

    Kojima, Naoto; Suga, Yuki; Matsumoto, Takuya; Tanaka, Tetsuaki; Akatsuka, Akinobu; Yamori, Takao; Dan, Shingo; Iwasaki, Hiroki; Yamashita, Masayuki

    2015-03-15

    The convergent synthesis of the dansyl-labeled probe of the thiophene-3-carboxamide analogue of annonaceous acetogenins, which shows potent antitumor activity, was accomplished by two asymmetric alkynylations of the 2,5-diformyl THF equivalent with an alkyne having a thiophene moiety and another alkyne tagged with a dansyl group. The growth inhibitory profiles toward 39 human cancer cell lines revealed that the probe retained the biological function of its mother compound, and would be useful for studying cellular activity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. MODERNIZATION OF TECHNOLOGICAL LINE FOR CELLULAR EXTRUSION PROCESS

    Directory of Open Access Journals (Sweden)

    Tomasz Garbacz

    2014-06-01

    As part of the modernization of the cellular extrusion technology the extrusion head was designed and made. During the designing and modeling of the head the Auto CAD programe was used. After the prototyping the extrusion head was tested. In the article specification of cellular extrusion process of thermoplastics was presented. In the research, the endothermal chemical blowing agents in amount 1,0% by mass were used. The quantity of used blowing agent has a direct influence on density and structure of the extruded product of modified polymers. However, these properties have further influence on porosity, impact strength, hardness, tensile strength and another.

  6. Development of vitrification line technology and the manufacture of equipment

    International Nuclear Information System (INIS)

    Alexa, J.

    1989-01-01

    The development is described of technology and the production of equipment for the vitrification of liquid radioactive wastes. For vitrification, frit Frita F270 is used containing up to 20% titanium and featuring a corrosion effect lower by one order than that of lead glass. The liquid waste is discharged in a measuring tank where it is mixed with formic acid. It is then pumped into an evaporator. Breed vapor is carried via a condenser to a condensate tank. The evaporator concentrate is transported to a homogenizer where it is gradually mixed with Frita. The viscous mush thus produced is carried into a furnace where the remaining water is evaporated. The furnace decontamination factor is 10 2 to 10 3 . At a temperature of up to 1,050 degC the frit melts and is discharged into a case. Currently, technology has been developed of mush preparation and the design has been completed of a vitrification furnace featuring remote lid opening and closing, and of equipment for processing furnace emissions. (J.B.). 3 figs., 1 tab., 1 ref

  7. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: Fourth Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2013-01-01

    SunLine Transit Agency, which provides public transit services to the Coachella Valley area of California, has demonstrated hydrogen and fuel cell bus technologies for more than 10 years. In May 2010, SunLine began demonstrating the advanced technology (AT) fuel cell bus with a hybrid electric propulsion system, fuel cell power system, and lithium-based hybrid batteries. This report describes operations at SunLine for the AT fuel cell bus and five compressed natural gas buses. The U.S. Department of Energy's National Renewable Energy Laboratory (NREL) is working with SunLine to evaluate the bus in real-world service to document the results and help determine the progress toward technology readiness. NREL has previously published three reports documenting the operation of the fuel cell bus in service. This report provides a summary of the results with a focus on the bus operation from February 2012 through November 2012.

  8. State of technology, system and solution supporting on-line maintenance - company's activities and products

    International Nuclear Information System (INIS)

    Nishitani, Junichi; Shimizu, Shunichi; Higasa, Hisakazu

    2010-01-01

    The new inspection system based on operator's maintenance and monitoring program of nuclear power plants was introduced in Japan more than one year ago and recommended on-line maintenance (maintenance during operation) will be carried out to increase capacity factor with safe and reliable operation of the plant. In this feature article, nine experts described the state of technology, system and solution supporting on-line maintenance - company's activities and products. These were titled as 'MHI's technology supporting on-line maintenance'. 'Technology supporting on-line maintenance - Toshiba's activities to upgrade monitoring and diagnostic service and maintenance management', 'AsahiKASEI's activities of on-line maintenance', 'Importance of information sharing of on-line maintenance and its ideal method-function of impact plan of IBM Maximo Asset Management for Nuclear', 'US's on-line maintenance and information systems', 'SmartProcedures realizing safe operation of nuclear power plant - proposal of computerized procedures', 'Ultrasonic leak detection system SDT170', 'Application of infrared thermography for equipment maintenance in nuclear power plant' and 'On-line condition monitoring system - condition eye'. (T. Tanaka)

  9. Current and Emerging Technologies for Probing Molecular Signatures of Traumatic Brain Injury

    Directory of Open Access Journals (Sweden)

    Ari Ercole

    2017-08-01

    Full Text Available Traumatic brain injury (TBI is understood as an interplay between the initial injury, subsequent secondary injuries, and a complex host response all of which are highly heterogeneous. An understanding of the underlying biology suggests a number of windows where mechanistically inspired interventions could be targeted. Unfortunately, biologically plausible therapies have to-date failed to translate into clinical practice. While a number of stereotypical pathways are now understood to be involved, current clinical characterization is too crude for it to be possible to characterize the biological phenotype in a truly mechanistically meaningful way. In this review, we examine current and emerging technologies for fuller biochemical characterization by the simultaneous measurement of multiple, diverse biomarkers. We describe how clinically available techniques such as cerebral microdialysis can be leveraged to give mechanistic insights into TBI pathobiology and how multiplex proteomic and metabolomic techniques can give a more complete description of the underlying biology. We also describe spatially resolved label-free multiplex techniques capable of probing structural differences in chemical signatures. Finally, we touch on the bioinformatics challenges that result from the acquisition of such large amounts of chemical data in the search for a more mechanistically complete description of the TBI phenotype.

  10. On Dwarves and Scientists: Probing for technological ethics in the creative imagination of J.R.R. Tolkien

    OpenAIRE

    Kidwell, Jeremy

    2009-01-01

    In this paper, I look more closely at J.R.R. Tolkien's writing to probe for a broader philosophy of technology. I suggest that (after a survey of his letters and literature), according to Tolkien none of the activities of technology, inquiry, making or use can be morally neutral. As a result, in Tolkien's account careful scrutiny must be made to ensure that these three types of technological activity occur within an ethically ordered economy, directed by appreciation for the intrinsic good of...

  11. Primary Multidrug Resistant Tuberculosis and Utility of Line Probe Assay for Its Detection in Smear-Positive Sputum Samples in a Tertiary Care Hospital in South India

    Directory of Open Access Journals (Sweden)

    Fahmiya Leena Yacoob

    2016-01-01

    Full Text Available In a high tuberculosis burdened country like India, rapid, cost-effective, and reliable diagnostic tools for tuberculosis are an urgent need of the hour to prevent inappropriate treatment strategies and further spread of resistance. This study aimed to estimate the proportion of new smear-positive tuberculosis cases with primary resistance to rifampicin and/or isoniazid as well as identify the common mutations associated with it. Sputum of 200 newly diagnosed smear-positive cases of 1+ score and above was directly subjected to Line Probe Assay using the GenoType MTBDRplus assay kit. All samples were inoculated onto solid media and 61 samples were inoculated in automated liquid culture also. The Line Probe Assay gave hundred percent interpretable results with 2.5% of the study population showing resistant pattern. Only 1% of the cases were primary multidrug resistant tuberculosis and 1.5% showed isoniazid monoresistance. S531L and C15T were the most common genetic mutations seen for rifampicin and isoniazid resistance, respectively. 40% had absent rpoB wild type 8 band indicating probable silent mutation after clinical correlation. The average turnaround time for Line Probe Assay was far less (3.8 days as compared to solid and liquid cultures (35.6 days and 13.5 days, resp..

  12. Primary Multidrug Resistant Tuberculosis and Utility of Line Probe Assay for Its Detection in Smear-Positive Sputum Samples in a Tertiary Care Hospital in South India.

    Science.gov (United States)

    Yacoob, Fahmiya Leena; Philomina Jose, Beena; Karunakaran Lelitha, Sarada Devi; Sreenivasan, Sreelatha

    2016-01-01

    In a high tuberculosis burdened country like India, rapid, cost-effective, and reliable diagnostic tools for tuberculosis are an urgent need of the hour to prevent inappropriate treatment strategies and further spread of resistance. This study aimed to estimate the proportion of new smear-positive tuberculosis cases with primary resistance to rifampicin and/or isoniazid as well as identify the common mutations associated with it. Sputum of 200 newly diagnosed smear-positive cases of 1+ score and above was directly subjected to Line Probe Assay using the GenoType MTBDRplus assay kit. All samples were inoculated onto solid media and 61 samples were inoculated in automated liquid culture also. The Line Probe Assay gave hundred percent interpretable results with 2.5% of the study population showing resistant pattern. Only 1% of the cases were primary multidrug resistant tuberculosis and 1.5% showed isoniazid monoresistance. S531L and C15T were the most common genetic mutations seen for rifampicin and isoniazid resistance, respectively. 40% had absent rpoB wild type 8 band indicating probable silent mutation after clinical correlation. The average turnaround time for Line Probe Assay was far less (3.8 days) as compared to solid and liquid cultures (35.6 days and 13.5 days, resp.).

  13. On-Line Monitoring of Environment-Assisted Cracking in Nuclear Piping Using Array Probe Direct Current Potential Drop

    OpenAIRE

    Kim, Y.; Choi, S.; Yoon, J. Y.; Nam, W. C.; Hwang, I. S.; Bromberg, Leslie; Stahle, Peter W; Ballinger, Ronald G

    2015-01-01

    A direct current potential drop method utilizing array probes with measurement ends maintaining an equalized potential designated as equi-potential switching array probe direct current potential drop (ESAP-DCPD) technique has been developed earlier at Seoul National University. This paper validates ESAP-DCPD technique by showing consistency among experimental measurements, analytical solution and numerical predictions using finite element analysis (FEA) of electric field changes with crack gr...

  14. Implementing Information Security and Its Technology: A LineManagement Perspective

    Energy Technology Data Exchange (ETDEWEB)

    Barletta, William A.

    2005-08-22

    Assuring the security and privacy of institutionalinformation assets is a complex task for the line manager responsible forinternational and multi-national transactions. In the face of an unsureand often conflicting international legal framework, the line managermust employ all available tools in an Integrated Security and PrivacyManagement framework that ranges from legal obligations, to policy, toprocedure, to cutting edge technology to counter the rapidly evolvingcyber threat to information assets and the physical systems thatinformation systems control.

  15. Advances in Mammalian Cell Line Development Technologies for Recombinant Protein Production

    Directory of Open Access Journals (Sweden)

    Say Kong Ng

    2013-04-01

    Full Text Available From 2006 to 2011, an average of 15 novel recombinant protein therapeutics have been approved by US Food and Drug Administration (FDA annually. In addition, the expiration of blockbuster biologics has also spurred the emergence of biosimilars. The increasing numbers of innovator biologic products and biosimilars have thus fuelled the demand of production cell lines with high productivity. Currently, mammalian cell line development technologies used by most biopharmaceutical companies are based on either the methotrexate (MTX amplification technology or the glutamine synthetase (GS system. With both systems, the cell clones obtained are highly heterogeneous, as a result of random genome integration by the gene of interest and the gene amplification process. Consequently, large numbers of cell clones have to be screened to identify rare stable high producer cell clones. As such, the cell line development process typically requires 6 to 12 months and is a time, capital and labour intensive process. This article reviews established advances in protein expression and clone screening which are the core technologies in mammalian cell line development. Advancements in these component technologies are vital to improve the speed and efficiency of generating robust and highly productive cell line for large scale production of protein therapeutics.

  16. Ultrahigh flux reactor design probing the limits of plate fuel technology

    International Nuclear Information System (INIS)

    Lake, J.A.; Parsons, D.K.; Liebenthal, J.L.; Ryskamp, J.M.; Fillmore, G.N.; Deboisblanc, D.R.

    1986-01-01

    The need for a new steady-state thermal neutron source of unprecedented intensity has been the subject of numerous national meetings and discussions. The National Research Council Committee on Major Facilities for Materials Research recently issued a high priority recommendation that site-independent design studies for such a facility begin immediately. The high intensity neutron source is projected to open new frontiers in the use of neutrons as a probe in various aspects of materials and biological research and fundamental physics. The challenge put forth by the research community is to produce a source with a tenfold increase in intensity over any currently operating or planned facility and, therefore, to thrust the thermal neutron flux intensity into the 10 16 n/(cm 2 s) range. The purpose of the recent Idaho National Engineering Laboratory (INEL) activities in this area has been to identify and examine the limitations and the capabilities of the historically well-characterized plate-fuel technology to achieve the required performance levels in a user-friendly environment. Workbench design concepts were identified, upon which constraints and performance limitations could be evaluated and parametric trade-off analyses and preliminary design optimization studies could be performed. Although considerable optimization remains to be performed and a large number of cost/benefit trade-offs exist, it appears that a reactor core with innovative geometry, constructed of plate-type fuel elements, can achieve the 10 16 n/(cm 2 s) goal thermal flux level in a large external volume which has the quality and accessibility for beam research. (orig.)

  17. A line technology of subterranean mining by means of nuclear explosions

    International Nuclear Information System (INIS)

    Gushchin, V.V.; Vasin, K.D.; Nifontov, B.I.; Odrov, Yu.L.; Myasnikov, K.V.; Kol'tsov, V.M.; Kornev, G.N.; Degtyarev, V.A.

    1976-01-01

    The basic principles of a line technology of extracting firm ores, including break-up of ore by means of subterranean nuclear explosions, the removal of ore from sorting blocks by means of vibrator feeders and transporting it to intermediate levels via a new type of belt conveyor on mobile supports are considered. A method of crushing ore by nuclear explosions on reflecting surfaces is proposed to obtain a more uniform fragmentation of ore, increase the output of fragmented ore per unit capacity, and to preserve mining output. The basic principles of designing a system of mining based on a line technology are formulated and one variant of such a system is presented

  18. An Experimental Protocol for Assessing the Performance of New Ultrasound Probes Based on CMUT Technology in Application to Brain Imaging.

    Science.gov (United States)

    Matrone, Giulia; Ramalli, Alessandro; Savoia, Alessandro Stuart; Quaglia, Fabio; Castellazzi, Gloria; Morbini, Patrizia; Piastra, Marco

    2017-09-24

    The possibility to perform an early and repeatable assessment of imaging performance is fundamental in the design and development process of new ultrasound (US) probes. Particularly, a more realistic analysis with application-specific imaging targets can be extremely valuable to assess the expected performance of US probes in their potential clinical field of application. The experimental protocol presented in this work was purposely designed to provide an application-specific assessment procedure for newly-developed US probe prototypes based on Capacitive Micromachined Ultrasonic Transducer (CMUT) technology in relation to brain imaging. The protocol combines the use of a bovine brain fixed in formalin as the imaging target, which ensures both realism and repeatability of the described procedures, and of neuronavigation techniques borrowed from neurosurgery. The US probe is in fact connected to a motion tracking system which acquires position data and enables the superposition of US images to reference Magnetic Resonance (MR) images of the brain. This provides a means for human experts to perform a visual qualitative assessment of the US probe imaging performance and to compare acquisitions made with different probes. Moreover, the protocol relies on the use of a complete and open research and development system for US image acquisition, i.e. the Ultrasound Advanced Open Platform (ULA-OP) scanner. The manuscript describes in detail the instruments and procedures involved in the protocol, in particular for the calibration, image acquisition and registration of US and MR images. The obtained results prove the effectiveness of the overall protocol presented, which is entirely open (within the limits of the instrumentation involved), repeatable, and covers the entire set of acquisition and processing activities for US images.

  19. Comparative Study of Biological and Technological Characters in Three Generations of Silkworm Bombyxmori L. Ameiotic, Parthenogenetically Cloned Lines

    OpenAIRE

    Greiss, H.; Vassilieva, J.; Petkov, N.; Petkov, Z.

    2004-01-01

    Purpose: Detect any deviation in biologic and technologic characters of eight ameiotic-parthenogenetically cloned lines of Bombyx mori L. from different origins from a normal sexually reproduced control line in three generations.

  20. Research and application on imaging technology of line structure light based on confocal microscopy

    Science.gov (United States)

    Han, Wenfeng; Xiao, Zexin; Wang, Xiaofen

    2009-11-01

    In 2005, the theory of line structure light confocal microscopy was put forward firstly in China by Xingyu Gao and Zexin Xiao in the Institute of Opt-mechatronics of Guilin University of Electronic Technology. Though the lateral resolution of line confocal microscopy can only reach or approach the level of the traditional dot confocal microscopy. But compared with traditional dot confocal microscopy, it has two advantages: first, by substituting line scanning for dot scanning, plane imaging only performs one-dimensional scanning, with imaging velocity greatly improved and scanning mechanism simplified, second, transfer quantity of light is greatly improved by substituting detection hairline for detection pinhole, and low illumination CCD is used directly to collect images instead of photoelectric intensifier. In order to apply the line confocal microscopy to practical system, based on the further research on the theory of the line confocal microscopy, imaging technology of line structure light is put forward on condition of implementation of confocal microscopy. Its validity and reliability are also verified by experiments.

  1. LINES

    Directory of Open Access Journals (Sweden)

    Minas Bakalchev

    2015-10-01

    Full Text Available The perception of elements in a system often creates their interdependence, interconditionality, and suppression. The lines from a basic geometrical element have become the model of a reductive world based on isolation according to certain criteria such as function, structure, and social organization. Their traces are experienced in the contemporary world as fragments or ruins of a system of domination of an assumed hierarchical unity. How can one release oneself from such dependence or determinism? How can the lines become less “systematic” and forms more autonomous, and less reductive? How is a form released from modernistic determinism on the new controversial ground? How can these elements or forms of representation become forms of action in the present complex world? In this paper, the meaning of lines through the ideas of Le Corbusier, Leonidov, Picasso, and Hitchcock is presented. Spatial research was made through a series of examples arising from the projects of the architectural studio “Residential Transformations”, which was a backbone for mapping the possibilities ranging from playfulness to exactness, as tactics of transformation in the different contexts of the contemporary world.

  2. Problems, pitfalls, and probes: Welcome to the jungle of electrochemical noise technology

    International Nuclear Information System (INIS)

    Edgemon, G.L.

    1998-01-01

    The rise in electrochemical noise (EN) as a corrosion monitoring technique has resulted in unique problems associated with the field application of this method. Many issues relate to the design of the EN probe electrodes. The ability of an electrochemical noise monitoring system to identify and discriminate between localized corrosion mechanisms is related primarily to the capability of the probe to separate the corrosion cell anode from the corresponding cathode. Effectiveness of this separation is largely determined by the details of and the proper design of the probe that is in the environment of interest. No single probe design or geometry can be effectively use in every situation to monitor all types of corrosion. In this paper the authors focus on a case study and probe development history related to monitoring corrosion in an extremely hostile environment using EN. While the ultimate application of EN was and continues to be successful, the case study shows that patience and persistence was necessary to meet and properly implement the monitoring program. Other possible source of problems and frustration with implementing EN are also discussed

  3. Monitoring of an esterification reaction by on-line direct liquid sampling mass spectrometry and in-line mid infrared spectrometry with an attenuated total reflectance probe

    International Nuclear Information System (INIS)

    Owen, Andrew W.; McAulay, Edith A.J.; Nordon, Alison; Littlejohn, David; Lynch, Thomas P.; Lancaster, J. Steven; Wright, Robert G.

    2014-01-01

    Highlights: • High efficiency thermal vaporiser designed and used for on-line reaction monitoring. • Concentration profiles of all reactants and products obtained from mass spectra. • By-product formed from the presence of an impurity detected by MS but not MIR. • Mass spectrometry can detect trace and bulk components unlike molecular spectrometry. - Abstract: A specially designed thermal vaporiser was used with a process mass spectrometer designed for gas analysis to monitor the esterification of butan-1-ol and acetic anhydride. The reaction was conducted at two scales: in a 150 mL flask and a 1 L jacketed batch reactor, with liquid delivery flow rates to the vaporiser of 0.1 and 1.0 mL min −1 , respectively. Mass spectrometry measurements were made at selected ion masses, and classical least squares multivariate linear regression was used to produce concentration profiles for the reactants, products and catalyst. The extent of reaction was obtained from the butyl acetate profile and found to be 83% and 76% at 40 °C and 20 °C, respectively, at the 1 L scale. Reactions in the 1 L reactor were also monitored by in-line mid-infrared (MIR) spectrometry; off-line gas chromatography (GC) was used as a reference technique when building partial least squares (PLS) multivariate calibration models for prediction of butyl acetate concentrations from the MIR spectra. In validation experiments, good agreement was achieved between the concentration of butyl acetate obtained from in-line MIR spectra and off-line GC. In the initial few minutes of the reaction the profiles for butyl acetate derived from on-line direct liquid sampling mass spectrometry (DLSMS) differed from those of in-line MIR spectrometry owing to the 2 min transfer time between the reactor and mass spectrometer. As the reaction proceeded, however, the difference between the concentration profiles became less noticeable. DLSMS had advantages over in-line MIR spectrometry as it was easier to generate

  4. A Survey of the Rapidly Emerging Field of Nanotechnology: Potential Applications for Scientific Instruments and Technologies for Atmospheric Entry Probes

    Science.gov (United States)

    Meyyappan, M.; Arnold, J. O.

    2005-01-01

    The field of Nanotechnology is well funded worldwide and innovations applicable to Solar System Exploration are emerging much more rapidly than thought possible just a few years ago. This presentation will survey recent innovations from nanotechnololgy with a focus on novel applications to atmospheric entry science and probe technology, in a fashion similar to that presented by Arnold and Venkatapathy at the previous workshop forum at Lisbon Portugal, October 6-9, 2003. Nanotechnology is a rapidly emerging field that builds systems, devices and materials from the bottom up, atom by atom, and in so doing provides them with novel and remarkable macro-scale performance. This technology has the potential to revolutionize space exploration by reducing mass and simultaneously increasing capability. Thermal, Radiation, Impact Protective Shields: Atmospheric probes and humans on long duration deep space missions involved in Solar System Exploration must safely endure 3 significant hazards: (i) atmospheric entry; (ii) radiation; and (iii) micrometeorite or debris impact. Nanostructured materials could be developed to address all three hazards with a single protective shield, which would involve much less mass than a traditional approach. The concept can be ready in time for incorporation into NASA s Crew Exploration Vehicle, and possible entry probes to fly on the Jupiter Icy Moons

  5. Solar Probe Plus: Report of the Science and Technology Definition Team

    Science.gov (United States)

    2008-01-01

    Solar Probe+ will be an extraordinary and historic mission, exploring what is arguably the last region of the solar system to be visited by a spacecraft, the Sun s outer atmosphere or corona as it extends out into space. Approaching as close as 9.5 RS* (8.5 RS above the Sun s surface), Solar Probe+ will repeatedly sample the near-Sun environment, revolutionizing our knowledge and understanding of coronal heating and of the origin and evolution of the solar wind and answering critical questions in heliophysics that have been ranked as top priorities for decades. Moreover, by making direct, in-situ measurements of the region where some of the most hazardous solar energetic particles are energized, Solar Probe+ will make a fundamental contribution to our ability to characterize and forecast the radiation environment in which future space explorers will work and live.

  6. Flow cytometric analysis of microbial contamination in food industry technological lines--initial study.

    Science.gov (United States)

    Józwa, Wojciech; Czaczyk, Katarzyna

    2012-04-02

    Flow cytometry constitutes an alternative for traditional methods of microorganisms identification and analysis, including methods requiring cultivation step. It enables the detection of pathogens and other microorganisms contaminants without the need to culture microbial cells meaning that the sample (water, waste or food e.g. milk, wine, beer) may be analysed directly. This leads to a significant reduction of time required for analysis allowing monitoring of production processes and immediate reaction in case of contamination or any disruption occurs. Apart from the analysis of raw materials or products on different stages of manufacturing process, the flow cytometry seems to constitute an ideal tool for the assessment of microbial contamination on the surface of technological lines. In the present work samples comprising smears from 3 different surfaces of technological lines from fruit and vegetable processing company from Greater Poland were analysed directly with flow cytometer. The measured parameters were forward and side scatter of laser light signals allowing the estimation of microbial cell contents in each sample. Flow cytometric analysis of the surface of food industry production lines enable the preliminary evaluation of microbial contamination within few minutes from the moment of sample arrival without the need of sample pretreatment. The presented method of fl ow cytometric initial evaluation of microbial state of food industry technological lines demonstrated its potential for developing a robust, routine method for the rapid and labor-saving detection of microbial contamination in food industry.

  7. Organizational and technological compatibility of the technological processes of second and third line maintenance of KhTZ-3522 tractors

    Directory of Open Access Journals (Sweden)

    Kyzminskyj Roman

    2018-01-01

    Full Text Available The work is devoted to systematic substantiation of productivity, production structure and specialization of service stations of KhTZ-3522 tractors. Based on the technical and economic comparative analysis of the results of the technological processes modeling of the second and third line maintenance of KhTZ-3522 tractors, the parametrical series of production structures of service stations both mono- and polytechnological specialization, which can work effectively in a wide range of the general annual program of orders, and in the conditions of seasonal fluctuations of orders for the maintenance of these tractors, are found. The results of calculations of the indicators system for organizational and technological compatibility of technological processes of the second and third line maintenance of KhTZ-3522 tractors in the joint technological flow are presented. The dependencies of these indicators on the total annual program of orders for service stations of different productivity are analyzed. According to the results of the analysis, the polytechnological specialization of such service stations is substantiated.

  8. On-line Technology Information System (OTIS): Solid Waste Management Technology Information Form (SWM TIF)

    Science.gov (United States)

    Levri, Julie A.; Boulanger, Richard; Hogan, John A.; Rodriguez, Luis

    2003-01-01

    Contents include the following: What is OTIS? OTIS use. Proposed implementation method. Development history of the Solid Waste Management (SWM) Technology Information Form (TIF) and OTIS. Current development state of the SWM TIF and OTIS. Data collection approach. Information categories. Critiques/questions/feedback.

  9. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: First Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2011-03-01

    This report describes operations at SunLine Transit Agency for their newest prototype fuel cell bus and five compressed natural gas (CNG) buses. In May 2010, SunLine began operating its sixth-generation hydrogen fueled bus, an Advanced Technology (AT) fuel cell bus that incorporates the latest design improvements to reduce weight and increase reliability and performance. The agency is collaborating with the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to evaluate the bus in revenue service. This report provides the early data results and implementation experience of the AT fuel cell bus since it was placed in service.

  10. Microfluidic technology platforms for synthesizing, labeling and measuring the kinetics of transport and biochemical reactions for developing molecular imaging probes

    Energy Technology Data Exchange (ETDEWEB)

    Phelps, Michael E. [Univ. of California, Los Angeles, CA (United States)

    2009-09-01

    Radiotracer techniques are used in environmental sciences, geology, biology and medicine. Radiotracers with Positron Emission Tomography (PET) provided biological examinations of ~3 million patients 2008. Despite the success of positron labeled tracers in many sciences, there is limited access in an affordable and convenient manner to develop and use new tracers. Integrated microfluidic chips are a new technology well matched to the concentrations of tracers. Our goal is to develop microfluidic chips and new synthesis approaches to enable wide dissemination of diverse types of tracers at low cost, and to produce new generations of radiochemists for which there are many unfilled jobs. The program objectives are to: 1. Develop an integrated microfluidic platform technology for synthesizing and 18F-labeling diverse arrays of different classes of molecules. 2. Incorporate microfluidic chips into small PC controlled devices (“Synthesizer”) with a platform interfaced to PC for electronic and fluid input/out control. 3. Establish a de-centralized model with Synthesizers for discovering and producing molecular imaging probes, only requiring delivery of inexpensive [18F]fluoride ion from commercial PET radiopharmacies vs the centralized approach of cyclotron facilities synthesizing and shipping a few different types of 18F-probes. 4. Develop a position sensitive avalanche photo diode (PSAPD) camera for beta particles embedded in a microfluidic chip for imaging and measuring transport and biochemical reaction rates to valid new 18F-labeled probes in an array of cell cultures. These objectives are met within a research and educational program integrating radio-chemistry, synthetic chemistry, biochemistry, engineering and biology in the Crump Institute for Molecular Imaging. The Radiochemistry Training Program exposes PhD and post doctoral students to molecular imaging in vitro in cells and microorganisms in microfluidic chips and in vivo with PET, from new technologies

  11. An electrochemical method for on-line monitoring of biofilm activity in cooling water using the BIoGEORGE trademark probe

    International Nuclear Information System (INIS)

    Licina, G.J.; Nekoksa, G.; Howard, R.L.

    1994-01-01

    The presence of active microorganisms on piping and components in cooling water systems can have a profound effect on the corrosion performance of such systems. Microbiologically influenced corrosion (MIC) can result in premature failures of critical and support systems, increased downtime of equipment for repairs and maintenance, and increased operating costs associated with mitigation measures. In some cases, MIC has forced premature replacement of tanks, heat exchangers, and piping systems with a severe effect on plant availability. Monitoring methods that alert plant operators that biofilm formation is occurring on pipe work and components permit the operators to initiate mitigation actions before biofouling becomes severe or MIC has occurred. An electrochemical probe to permit on-line monitoring of biofilm activity under power plant or other industrial exposure conditions is under development. This device, the BIoGEORGE trademark electrochemical biofilm monitor, permits on-line evaluations of the effects of biofilm formation upon the surfaces of passive alloys such as stainless steels exposed to cooling water environments. Benchtop experiments have shown that biofilm formation on stainless steel surfaces can be detected by an electrochemical indication well in advance of any visual evidence of biofilm or corrosion on the electrodes. The design of the probe, results of benchtop experiments, and a description of its installation at the Browns Ferry Nuclear Plant are described

  12. Magnetic field effects on spectrally resolved lifetime of on-line oxygen monitoring using magneto-optic probes

    Science.gov (United States)

    Mermut, O.; Gallant, P.; Le Bouch, N.; Leclair, S.; Noiseux, I.; Vernon, M.; Morin, J.-F.; Diamond, K.; Patterson, M. S.; Samkoe, K.; Pogue, B.

    2009-02-01

    Multimodal agents that serve as both probes for contrast and light-activated effectors of cellular processes in diseased tissue were developed. These agents were introduced into multicellular tumor spheroids (3D tissue models) and in the chorioallantoic membrane (CAM) of a chicken embryo. The luminescence decay was examined using a novel technique involving a spectrally-resolved fluorescence lifetime apparatus integrated with a weak electromagnet. A spectrallyresolved lifetime setup was used to identify magneto-optic species sensitive to magnetic field effects and distinguish from background emissions. We demonstrate that the applied magnetic fields can alter reaction rates and product distribution of some dyes detected by time- and spectrally-resolved luminescence changes. We will discuss the use of exogenous magneto-optical probes taken up in tumors to both induce phototoxicity, a process that is governed by complex and dynamically evolving mechanisms involving reactive oxygen species, and monitor treatment progress. The magnetic field enhancement, measured over a range of weak fields (0-300 mT) is correlated to oxygenation and may be used to monitor dynamic changes occurring due to oxygen consumption over the course of photodynamic therapy. Such online measurements provide the possibility to derive real-time information about response to treatment via monitoring magnetic field enhancement/suppression of the time-resolved, spectrally-resolved luminescence of the probe at the site of the treatment directly. Magnetic perturbation of lifetime can serve as a status reporter, providing optical feedback of oxygen-mediated treatments in situ and allowing for real-time adjustment of a phototherapy treatment plan.

  13. Bottom-Up Technologies for Reuse: Automated Extractive Adoption of Software Product Lines

    OpenAIRE

    Martinez , Jabier ,; Ziadi , Tewfik; Bissyandé , Tegawendé; Klein , Jacques ,; Le Traon , Yves ,

    2017-01-01

    International audience; Adopting Software Product Line (SPL) engineering principles demands a high up-front investment. Bottom-Up Technologies for Reuse (BUT4Reuse) is a generic and extensible tool aimed to leverage existing similar software products in order to help in extractive SPL adoption. The envisioned users are 1) SPL adopters and 2) Integrators of techniques and algorithms to provide automation in SPL adoption activities. We present the methodology it implies for both types of users ...

  14. Critical parameters affecting the design of high frequency transmission lines in standard CMOS technology

    KAUST Repository

    Al Attar, Talal

    2017-05-13

    Different structures of transmission lines were designed and fabricated in standard CMOS technology to estimate some critical parameters including the RMS value of the surface roughness and the loss tangent. The input impedances for frequencies up to 50 GHz were modeled and compared with measurements. The results demonstrated a strong correlation between the used model with the proposed coefficients and the measured results, attesting the robustness of the model and the reliability of the incorporated coefficients values.

  15. Critical parameters affecting the design of high frequency transmission lines in standard CMOS technology

    KAUST Repository

    Al Attar, Talal; Alshehri, Abdullah; Almansouri, Abdullah Saud Mohammed; Al-Turki, Abdullah Turki

    2017-01-01

    Different structures of transmission lines were designed and fabricated in standard CMOS technology to estimate some critical parameters including the RMS value of the surface roughness and the loss tangent. The input impedances for frequencies up to 50 GHz were modeled and compared with measurements. The results demonstrated a strong correlation between the used model with the proposed coefficients and the measured results, attesting the robustness of the model and the reliability of the incorporated coefficients values.

  16. On-line Delivery for Serbo-Croatian (Bosniac, Croatian, Serbian: Tailoring Technology to the Learner

    Directory of Open Access Journals (Sweden)

    Danko Sipka

    2004-01-01

    Full Text Available Tbis paper discusses the project of full on-line delivery of an introductory Serbo-Croatian (Bosniac, Croatian, Serbian course at the Arizona State University Critical Languages Institute. The issue of tailoring technology to meet diversified needs of a wide range of learners (from heritage speakers of the three Serbo-Croatian speaking ethnic communities to professional non-heritage learners is in the center of the discussion.

  17. Bead-probe complex capture a couple of SINE and LINE family from genomes of two closely related species of East Asian cyprinid directly using magnetic separation

    Science.gov (United States)

    Tong, Chaobo; Guo, Baocheng; He, Shunping

    2009-01-01

    Background Short and long interspersed elements (SINEs and LINEs, respectively), two types of retroposons, are active in shaping the architecture of genomes and powerful tools for studies of phylogeny and population biology. Here we developed special protocol to apply biotin-streptavidin bead system into isolation of interspersed repeated sequences rapidly and efficiently, in which SINEs and LINEs were captured directly from digested genomic DNA by hybridization to bead-probe complex in solution instead of traditional strategy including genomic library construction and screening. Results A new couple of SINEs and LINEs that shared an almost identical 3'tail was isolated and characterized in silver carp and bighead carp of two closely related species. These SINEs (34 members), designated HAmo SINE family, were little divergent in sequence and flanked by obvious TSD indicated that HAmo SINE was very young family. The copy numbers of this family was estimated to 2 × 105 and 1.7 × 105 per haploid genome by Real-Time qPCR, respectively. The LINEs, identified as the homologs of LINE2 in other fishes, had a conserved primary sequence and secondary structures of the 3'tail region that was almost identical to that of HAmo SINE. These evidences suggest that HAmo SINEs are active and amplified recently utilizing the enzymatic machinery for retroposition of HAmoL2 through the recognition of higher-order structures of the conserved 42-tail region. We analyzed the possible structures of HAmo SINE that lead to successful amplification in genome and then deduced that HAmo SINE, SmaI SINE and FokI SINE that were similar in sequence each other, were probably generated independently and created by LINE family within the same lineage of a LINE phylogeny in the genomes of different hosts. Conclusion The presented results show the advantage of the novel method for retroposons isolation and a pair of young SINE family and its partner LINE family in two carp fishes, which strengthened

  18. Bead-probe complex capture a couple of SINE and LINE family from genomes of two closely related species of East Asian cyprinid directly using magnetic separation

    Directory of Open Access Journals (Sweden)

    Guo Baocheng

    2009-02-01

    Full Text Available Abstract Background Short and long interspersed elements (SINEs and LINEs, respectively, two types of retroposons, are active in shaping the architecture of genomes and powerful tools for studies of phylogeny and population biology. Here we developed special protocol to apply biotin-streptavidin bead system into isolation of interspersed repeated sequences rapidly and efficiently, in which SINEs and LINEs were captured directly from digested genomic DNA by hybridization to bead-probe complex in solution instead of traditional strategy including genomic library construction and screening. Results A new couple of SINEs and LINEs that shared an almost identical 3'tail was isolated and characterized in silver carp and bighead carp of two closely related species. These SINEs (34 members, designated HAmo SINE family, were little divergent in sequence and flanked by obvious TSD indicated that HAmo SINE was very young family. The copy numbers of this family was estimated to 2 × 105 and 1.7 × 105 per haploid genome by Real-Time qPCR, respectively. The LINEs, identified as the homologs of LINE2 in other fishes, had a conserved primary sequence and secondary structures of the 3'tail region that was almost identical to that of HAmo SINE. These evidences suggest that HAmo SINEs are active and amplified recently utilizing the enzymatic machinery for retroposition of HAmoL2 through the recognition of higher-order structures of the conserved 42-tail region. We analyzed the possible structures of HAmo SINE that lead to successful amplification in genome and then deduced that HAmo SINE, SmaI SINE and FokI SINE that were similar in sequence each other, were probably generated independently and created by LINE family within the same lineage of a LINE phylogeny in the genomes of different hosts. Conclusion The presented results show the advantage of the novel method for retroposons isolation and a pair of young SINE family and its partner LINE family in two carp

  19. On-line sensor technology for food manufacturing industry. Shokuhin bun prime ya ni okeru on-line sensor gijutsu

    Energy Technology Data Exchange (ETDEWEB)

    Komiya, K. (Ajinomoto Co. Inc., Tokyo (Japan))

    1990-08-05

    This paper introduced the sensor technology for food manufacturing industry. If sugar concentration in main raw material is too high in the amino acid fermentation, control of the concentration is required because fungi growth is inhibited. A controlling method for sugar concentration was developed by using the correlation between consumption of NH {sub 3} for pH adjustment and sugar consumption in place of conventional analyzing method and was introduced in the gulutamic acid fermantation. BOD sensor was developed to enable the selective measurement of organic substances which can be processed by organisms, and measuring time was shortened from previous five days to 30 minutes. Since many organics absorb infrared ray, near infrared analysis is suitable for food analysis and on-line analysis has high possibility. When this method is applied to measure moisture in fishes and meats, continuous measurement can be made nondestructively and without contacting, and further the precision is {plus minus} 0.1%. Simultaneous multi-composition analyses are carried out by continuous spectrum and near infrared method using higher rate scanning. Sensor development for taste and smell has started. 10 refs., 4 figs., 4 refs.

  20. Amide I SFG Spectral Line Width Probes the Lipid-Peptide and Peptide-Peptide Interactions at Cell Membrane In Situ and in Real Time.

    Science.gov (United States)

    Zhang, Baixiong; Tan, Junjun; Li, Chuanzhao; Zhang, Jiahui; Ye, Shuji

    2018-06-13

    The balance of lipid-peptide and peptide-peptide interactions at cell membrane is essential to a large variety of cellular processes. In this study, we have experimentally demonstrated for the first time that sum frequency generation vibrational spectroscopy can be used to probe the peptide-peptide and lipid-peptide interactions in cell membrane in situ and in real time by determination of the line width of amide I band of protein backbone. Using a "benchmark" model of α-helical WALP23, it is found that the dominated lipid-peptide interaction causes a narrow line width of the amide I band, whereas the peptide-peptide interaction can markedly broaden the line width. When WALP23 molecules insert into the lipid bilayer, a quite narrow line width of the amide I band is observed because of the lipid-peptide interaction. In contrast, when the peptide lies down on the bilayer surface, the line width of amide I band becomes very broad owing to the peptide-peptide interaction. In terms of the real-time change in the line width, the transition from peptide-peptide interaction to lipid-peptide interaction is monitored during the insertion of WALP23 into 1,2-dipalmitoyl- sn-glycero-3-phospho-(1'- rac-glycerol) (DPPG) lipid bilayer. The dephasing time of a pure α-helical WALP23 in 1-palmitoyl-2-oleoyl- sn-glycero-3-phospho-(1'- rac-glycerol) and DPPG bilayer is determined to be 2.2 and 0.64 ps, respectively. The peptide-peptide interaction can largely accelerate the dephasing time.

  1. Physical properties in flux line lattice state in MgB2 probed by μSR

    International Nuclear Information System (INIS)

    Ohishi, Kazuki; Muranaka, Takahiro; Akimitsu, Jun; Koda, Akihiro; Higemoto, Wataru; Kadono, Ryosuke

    2002-01-01

    We have performed muon spin rotation (μSR) measurements to deduce the magnetic penetration depth λ in the flux line lattice state of MgB 2 microscopically. It is observed that λ shows a quadratic temperature dependence which is predicted for the case of superconducting gap with line nodes. Furthermore, it clearly exhibits a strong field dependence, where λ increases almost linearly with H. These results strongly suggest that the superconducting order parameter in MgB 2 is highly anisotropic. (author)

  2. A Technology Development Roadmap for a Near-Term Probe-Class X-ray Astrophysics Mission

    Science.gov (United States)

    Daelemans, Gerard J.; Petre, Robert; Bookbinder, Jay; Ptak, Andrew; Smith, Randall

    2013-01-01

    This document presents a roadmap, including proposed budget and schedule, for maturing the instrumentation needed for an X-ray astrophysics Probe-class mission. The Physics of the Cosmos (PCOS) Program Office was directed to create this roadmap following the December 2012 NASA Astrophysics Implementation Plan (AIP). Definition of this mission is called for in the AIP, with the possibility of selection in 2015 for a start in 2017. The overall mission capabilities and instrument performance requirements were defined in the 2010 Astronomy and Astrophysics Decadal Survey report, New Worlds, New Horizons in Astronomy and Astrophysics (NWNH), in connection with the highly ranked International X-ray Observatory (IXO). In NWNH, recommendations were provided regarding the size of, and instrumentation needed by, the next large X-ray observatory. Specifically, the key instrumental capability would be an X-ray calorimeter spectrometer at the focus of a large mirror with angular resolution of 10 arc seconds (arcsec) or better. If possible, a grating spectrometer should also be incorporated into the instrument complement. In response to these recommendations, four instrumentation technologies are included in this roadmap. Three of these are critical for an X-ray mission designed to address NWNH questions: segmented X-ray mirrors, transition edge sensor calorimeters, and gratings. Two approaches are described for gratings, which represent the least mature technology and thus most in need of a parallel path for risk reduction. Also, while current CCD detectors would likely meet the mission needs for grating spectrum readout, specific improvements are included as an additional approach for achieving the grating system effective area requirement. The technical steps needed for these technologies to attain technology readiness levels (TRL) of 5 and 6 are described, as well as desirable modest risk reduction steps beyond TRL-6. All of the technology development efforts are currently

  3. Probing the accretion flow and emission-line regions of M81, the nearest broad-lined low-luminosity AGN

    Science.gov (United States)

    Barth, Aaron

    2017-08-01

    The nucleus of M81 is an object of singular importance as a template for low-luminosity accretion flows onto supermassive black holes. We propose to obtain a complete, small-aperture, high S/N STIS UV/optical spectrum of the M81 nucleus and multi-filter WFC3 imaging covering the UV through near-IR. Such data have never previously been obtained with HST; the only prior archival UV/optical spectra of M81 have low S/N, incomplete wavelength coverage, and are strongly contaminated by starlight. Combined with new Chandra X-ray data, our proposed observations will comprise the definitive reference dataset on the spectral energy distribution of this benchmark low-luminosity AGN. These data will provide unique new constraints on the possible contribution of a truncated thin accretion disk to the AGN emission spectrum, clarifying a fundamental property of low-luminosity accretion flows. The data will additionally provide new insights into broad-line region structure and black hole mass scaling relationships at the lowest AGN luminosities, and spatially resolved diagnostics of narrow-line region excitation conditions at unprecedented spatial resolution to assess the impact of the AGN on the ionization state of the gas in the host galaxy bulge.

  4. Exploring Students’ Intention to Use LINE for Academic Purposes Based on Technology Acceptance Model

    Directory of Open Access Journals (Sweden)

    Willard Van De Bogart

    2015-06-01

    Full Text Available The LINE application is often conceived as purely social space; however, the authors of this paper wanted to determine if it could be used for academic purposes. In this study, we examined how undergraduate students accepted LINE in terms of using it for classroom-related activities (e.g., submit homework, follow up course information queries, download materials and explored the factors that might affect their intention to use it. Data were collected from 144 undergraduate students enrolled in an English course that utilized some activities based on LINE app using a questionnaire developed from TAM. Data were analyzed to see if relationships existed among factors when LINE was used to organize classroom experiences. The findings revealed that perceived usefulness and attitude toward usage had positive relationships with intention to use while perceived ease of use was positively related to perceived usefulness. In contrast with TAM assertions, this study did not find any relationship between perceived ease of use and attitude toward usage. Also, the number of social networking sites that students are using had no relationship with intention to use. The study also suggested some kinds of LINE-based learning activities preferred by students, which would be proposed for future courses. This study revealed several useful implications that TAM can be employed as a useful theoretical framework to predict and understand users’ intention to use new technologies in education.

  5. R and D of On-line Reprocessing Technology for Molten-Salt Reactor Systems

    International Nuclear Information System (INIS)

    Uhlir, Jan; Tulackova, Radka; Chuchvalcova Bimova, Karolina

    2006-01-01

    The Molten Salt Reactor (MSR) represents one of promising future nuclear reactor concept included in the Generation IV reactors family. The reactor can be operated as the thorium breeder or as the actinide transmuter. However, the future deployment of Molten-Salt Reactors will be significantly dependent on the successful mastering of advanced reprocessing technologies dedicated to their fuel cycle. Here the on-line reprocessing technology connected with the fuel circuit of MSR is of special importance because the reactor cannot be operated for a long run without the fuel salt clean-up. Generally, main MSR reprocessing technologies are pyrochemical, majority of them are fluoride technologies. The proposed flow-sheets of MSR on-line reprocessing are based on a combination of molten-salt / liquid metal extraction and electro-separation processes, which can be added to the gas extraction process already verified during the MSRE project in ORNL. The crucial separation method proposed for partitioning of actinides from fission products is based on successive Anodic dissolution and Cathodic deposition processes in molten fluoride media. (authors)

  6. Report from the Light Water Reactor Sustainability Workshop on On-Line Monitoring Technologies

    International Nuclear Information System (INIS)

    Baldwin, Thomas; Tawfik, Magdy; Bond, Leonard

    2010-01-01

    In support of expanding the use of nuclear power, interest is growing in methods of determining the feasibility of longer term operation for the U.S. fleet of nuclear power plants, particularly operation beyond 60 years. To help establish the scientific and technical basis for such longer term operation, the DOE-NE has established a research and development (R and D) objective. This objective seeks to develop technologies and other solutions that can improve the reliability, sustain the safety, and extend the life of current reactors. The Light Water Reactor Sustainability (LWRS) Program, which addresses the needs of this objective, is being developed in collaboration with industry R and D programs to provide the technical foundations for licensing and managing the long-term, safe, and economical operation of nuclear power plants. The LWRS Program focus is on longer-term and higher-risk/reward research that contributes to the national policy objectives of energy and environmental security. In moving to identify priorities and plan activities, the Light Water Reactor Sustainability Workshop on On-Line Monitoring (OLM) Technologies was held June 10-12, 2010, in Seattle, Washington. The workshop was run to enable industry stakeholders and researchers to identify the nuclear industry needs in the areas of future OLM technologies and corresponding technology gaps and research capabilities. It also sought to identify approaches for collaboration that would be able to bridge or fill the technology gaps. This report is the meeting proceedings, documenting the presentations and discussions of the workshop and is intended to serve as a basis for a plan which is under development that will enable the I and C research pathway to achieve its goals. Benefits to the nuclear industry accruing from On Line Monitoring Technology cannot be ignored. Information gathered thus far has contributed significantly to the Department of Energy's Light Water Reactor Sustainability Program. DOE

  7. Report from the Light Water Reactor Sustainability Workshop on On-Line Monitoring Technologies

    Energy Technology Data Exchange (ETDEWEB)

    Thomas Baldwin; Magdy Tawfik; Leonard Bond

    2010-06-01

    In support of expanding the use of nuclear power, interest is growing in methods of determining the feasibility of longer term operation for the U.S. fleet of nuclear power plants, particularly operation beyond 60 years. To help establish the scientific and technical basis for such longer term operation, the DOE-NE has established a research and development (R&D) objective. This objective seeks to develop technologies and other solutions that can improve the reliability, sustain the safety, and extend the life of current reactors. The Light Water Reactor Sustainability (LWRS) Program, which addresses the needs of this objective, is being developed in collaboration with industry R&D programs to provide the technical foundations for licensing and managing the long-term, safe, and economical operation of nuclear power plants. The LWRS Program focus is on longer-term and higher-risk/reward research that contributes to the national policy objectives of energy and environmental security. In moving to identify priorities and plan activities, the Light Water Reactor Sustainability Workshop on On-Line Monitoring (OLM) Technologies was held June 10–12, 2010, in Seattle, Washington. The workshop was run to enable industry stakeholders and researchers to identify the nuclear industry needs in the areas of future OLM technologies and corresponding technology gaps and research capabilities. It also sought to identify approaches for collaboration that would be able to bridge or fill the technology gaps. This report is the meeting proceedings, documenting the presentations and discussions of the workshop and is intended to serve as a basis for a plan which is under development that will enable the I&C research pathway to achieve its goals. Benefits to the nuclear industry accruing from On Line Monitoring Technology cannot be ignored. Information gathered thus far has contributed significantly to the Department of Energy’s Light Water Reactor Sustainability Program. DOE has

  8. Probing the sky with radio waves from wireless technology to the development of atmospheric science

    CERN Document Server

    Yeang, Chen-Pang

    2013-01-01

    By the late nineteenth century, engineers and experimental scientists generally knew how radio waves behaved, and by 1901 scientists were able to manipulate them to transmit messages across long distances. What no one could understand, however, was why radio waves followed the curvature of the Earth. Theorists puzzled over this for nearly twenty years before physicists confirmed the zig-zag theory, a solution that led to the discovery of a layer in the Earth's upper atmosphere that bounces radio waves earthward-the ionosphere. In Probing the Sky with Radio Waves,

  9. Development of on-line heavy water analysis by vibrating probe density meter and multiple internal reflectance infrared spectrometry

    International Nuclear Information System (INIS)

    Jones, V.D.; Nora, B.

    1984-01-01

    Achieving high productivity in the Savannah River Plant nuclear reactors requires that the heavy water (D 2 O) moderator be maintained at a high purity level. Since the D 2 O purity will degrade with time, a fraction of the moderator must be continually reprocessed to remove H 2 O. This rework process uses a series of fractional distillation columns. The process control is based on laboratory analyses of process samples every four hours. The sample streams, which can range from 0.10 to 99.80 mol % D 2 O, are analyzed using infrared spectrophotometry. An automatic on-line analysis would provide tighter process control and reduce personnel exposure to the tritiated moderator. Two instruments are being evaluated for on-line control; an Anton/Parr DPR 2000 density measuring system and a General Analysis Corporation LAN-I infrared liquid stream monitor

  10. Evaluating Assistive Technology in Early Childhood Education: The Use of a Concurrent Time Series Probe Approach

    Science.gov (United States)

    Parette, Howard P.; Blum, Craig; Boeckmann, Nichole M.

    2009-01-01

    As assistive technology applications are increasingly implemented in early childhood settings for children who are at risk or who have disabilities, it is critical that teachers utilize observational approaches to determine whether targeted assistive technology-supported interventions make a difference in children's learning. One structured…

  11. Applying ultrasonic in-line inspection technology in a deep water environment: exploring the challenges

    Energy Technology Data Exchange (ETDEWEB)

    Thielager, N.; Nadler, M.; Pieske, M.; Beller, M. [NDT Systems and Services AG, Stutensee (Germany)

    2009-12-19

    The demand for higher inspection accuracies of in-line inspection tools (ILI tools) is permanently growing. As integrity assessment procedures are being refined, detection performances, sizing accuracies and confidence levels regarding detection and sizing play an ever increasing role. ILI tools utilizing conventional ultrasound technology are at the forefront of technology and fulfill the market requirements regarding sizing accuracies and the ability to provide quantitative measurements of wall thickness as well as crack inspection capabilities. Data from ultrasonic tools is ideally suited for advanced integrity assessment applications and run comparisons. Making this technology available for a deep-water environment of heavy wall, high pressures and temperatures comes with a wide range of challenges which have to be addressed. This paper will introduce developments recently made in order to adapt and modify ultrasonic in-line inspection tools for the application in a heavy wall, high pressure and high temperature environment as encountered in deep offshore pipelines. The paper will describe necessary design modifications and new conceptual approaches especially regarding tool electronics, cables, connectors and the sensor carrier. A tool capable of deep-water inspection with a pressure bearing capability of 275 bar will be introduced and data from inspection runs will be presented. As an outlook, the paper will also discuss future inspection requirements for offshore pipelines with maximum pressure values of up to 500 bar. (author)

  12. Process Analytical Technology for High Shear Wet Granulation: Wet Mass Consistency Reported by In-Line Drag Flow Force Sensor Is Consistent With Powder Rheology Measured by At-Line FT4 Powder Rheometer.

    Science.gov (United States)

    Narang, Ajit S; Sheverev, Valery; Freeman, Tim; Both, Douglas; Stepaniuk, Vadim; Delancy, Michael; Millington-Smith, Doug; Macias, Kevin; Subramanian, Ganeshkumar

    2016-01-01

    Drag flow force (DFF) sensor that measures the force exerted by wet mass in a granulator on a thin cylindrical probe was shown as a promising process analytical technology for real-time in-line high-resolution monitoring of wet mass consistency during high shear wet granulation. Our previous studies indicated that this process analytical technology tool could be correlated to granulation end point established independently through drug product critical quality attributes. In this study, the measurements of flow force by a DFF sensor, taken during wet granulation of 3 placebo formulations with different binder content, are compared with concurrent at line FT4 Powder Rheometer characterization of wet granules collected at different time points of the processing. The wet mass consistency measured by the DFF sensor correlated well with the granulation's resistance to flow and interparticulate interactions as measured by FT4 Powder Rheometer. This indicated that the force pulse magnitude measured by the DFF sensor was indicative of fundamental material properties (e.g., shear viscosity and granule size/density), as they were changing during the granulation process. These studies indicate that DFF sensor can be a valuable tool for wet granulation formulation and process development and scale up, as well as for routine monitoring and control during manufacturing. Copyright © 2016. Published by Elsevier Inc.

  13. Radiation source states on-line supervision system design and implementation based on RFID technology

    International Nuclear Information System (INIS)

    Yang Binhua; Ling Qiu; Yin Guoli; Yang Kun; Wan Xueping; Wang Kan

    2011-01-01

    It puts forward radiation source states on-line monitoring resolution based on RFID technology. Firstly, the system uses RFID in real-time transmission of the radiation dose rate, and monitors the radiation source states and dose rate of the surrounding environment on-line. Then it adopts regional wireless networking mode to construct enterprise level monitoring network, which resolves long-distance wiring problems. And then it uses GPRS wireless to transport the real-time data to the monitoring center and the government supervision department, By adopting randomly dynamic cording in display update every day, it strengthens the supervision of the radiation source. At last this system has been successful applied to a thickness gauge project, which verifies the feasibility and practicality is good. (authors)

  14. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: Third Results Reports

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2012-05-01

    This report describes operations at SunLine Transit Agency for their newest prototype fuel cell bus and five compressed natural gas (CNG) buses. In May 2010, SunLine began operating its sixth-generation hydrogen fueled bus, an Advanced Technology (AT) fuel cell bus that incorporates the latest design improvements to reduce weight and increase reliability and performance. The agency is collaborating with the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to evaluate the bus in revenue service. NREL has previously published two reports documenting the operation of the fuel cell bus in service. This report provides a summary of the results with a focus on the bus operation from July 2011 through January 2012.

  15. Process Analytical Technology and On-Line Spectroscopic Measurements of Chemical Meat Quality

    DEFF Research Database (Denmark)

    Sørensen, Klavs Martin

    This thesis deals with process analytical technology and how it can be implemented in the meat industry through on-line grading of chemical meat quality. The focus will be on two applications, namely the rapid quality control of fat quality and the development of a method for on-line detection...... of nano-molar quantification in few seconds, in addition to an accelerated extraction-free GC-MS method that through automation can deliver results much faster than other similar methods. The implementation of these high tech methods will provide the meat industry with a leading edge not only with product...... of boar taint. The chemical makeup of fat has a large effect on meat cut quality. Fat quality has traditionally been determined by methylation of a tissue sample followed by chromatography on a GC-MS system, elucidating the composition of the individual fatty acids. As this procedure typically takes far...

  16. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: Second Results Report and Appendices

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2011-10-01

    This report describes operations at SunLine Transit Agency for their newest prototype fuel cell bus and five compressed natural gas (CNG) buses. In May 2010, SunLine began operating its sixth-generation hydrogen fueled bus, an Advanced Technology (AT) fuel cell bus that incorporates the latest design improvements to reduce weight and increase reliability and performance. The agency is collaborating with the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to evaluate the bus in revenue service. This is the second results report for the AT fuel cell bus since it was placed in service, and it focuses on the newest data analysis and lessons learned since the previous report. The appendices, referenced in the main report, provide the full background for the evaluation. They will be updated as new information is collected but will contain the original background material from the first report.

  17. Looking into individual coffee beans during the roasting process: direct micro-probe sampling on-line photo-ionisation mass spectrometric analysis of coffee roasting gases.

    Science.gov (United States)

    Hertz-Schünemann, Romy; Streibel, Thorsten; Ehlert, Sven; Zimmermann, Ralf

    2013-09-01

    A micro-probe (μ-probe) gas sampling device for on-line analysis of gases evolving in confined, small objects by single-photon ionisation time-of-flight mass spectrometry (SPI-TOFMS) was developed. The technique is applied for the first time in a feasibility study to record the formation of volatile and flavour compounds during the roasting process within (inside) or in the direct vicinity (outside) of individual coffee beans. A real-time on-line analysis of evolving volatile and semi-volatile organic compounds (VOC and SVOC) as they are formed under the mild pyrolytic conditions of the roasting process was performed. The soft-ionisation mass spectra depict a molecular ion signature, which is well corresponding with the existing knowledge of coffee roasting and evolving compounds. Additionally, thereby it is possible to discriminate between Coffea arabica (Arabica) and Coffea canephora (Robusta). The recognized differences in the roasting gas profiles reflect the differences in the precursor composition of the coffee cultivars very well. Furthermore, a well-known set of marker compounds for Arabica and Robusta, namely the lipids kahweol and cafestol (detected in their dehydrated form at m/z 296 and m/z 298, respectively) were observed. If the variation in time of different compounds is observed, distinctly different evolution behaviours were detected. Here, phenol (m/z 94) and caffeine (m/z 194) are exemplary chosen, whereas phenol shows very sharp emission peaks, caffeine do not have this highly transient behaviour. Finally, the changes of the chemical signature as a function of the roasting time, the influence of sampling position (inside, outside) and cultivar (Arabica, Robusta) is investigated by multivariate statistics (PCA). In summary, this pilot study demonstrates the high potential of the measurement technique to enhance the fundamental knowledge of the formation processes of volatile and semi-volatile flavour compounds inside the individual coffee bean.

  18. Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background.

    Science.gov (United States)

    Carstea, Ana Claudia; Pirity, Melinda K; Dinnyes, Andras

    2009-12-31

    In mice, gene targeting by homologous recombination continues to play an essential role in the understanding of functional genomics. This strategy allows precise location of the site of transgene integration and is most commonly used to ablate gene expression ("knock-out"), or to introduce mutant or modified alleles at the locus of interest ("knock-in"). The efficacy of producing live, transgenic mice challenges our understanding of this complex process, and of the factors which influence germline competence of embryonic stem cell lines. Increasingly, evidence indicates that culture conditions and in vitro manipulation can affect the germline-competence of Embryonic Stem cell (ES cell) lines by accumulation of chromosome abnormalities and/or epigenetic alterations of the ES cell genome. The effectiveness of ES cell derivation is greatly strain-dependent and it may also influence the germline transmission capability. Recent technical improvements in the production of germline chimeras have been focused on means of generating ES cells lines with a higher germline potential. There are a number of options for generating chimeras from ES cells (ES chimera mice); however, each method has its advantages and disadvantages. Recent developments in induced pluripotent stem (iPS) cell technology have opened new avenues for generation of animals from genetically modified somatic cells by means of chimera technologies. The aim of this review is to give a brief account of how the factors mentioned above are influencing the germline transmission capacity and the developmental potential of mouse pluripotent stem cell lines. The most recent methods for generating specifically ES and iPS chimera mice, including the advantages and disadvantages of each method are also discussed.

  19. Speed dependence of CH335Cl–O2 line-broadening parameters probed on rotational transitions: Measurements and semi-classical calculations

    International Nuclear Information System (INIS)

    Buldyreva, J.; Margulès, L.; Motiyenko, R.A.; Rohart, F.

    2013-01-01

    Relaxation parameters for K-components (K≤6) of six J→J+1 rotational transitions (J=6, 10, 17, 22, 31 and 33) of CH 3 35 Cl perturbed by O 2 are measured at room temperature with Voigt, speed-dependent Voigt and Galatry profiles in order to probe the speed-dependence effects. With respect to the previous study of CH 3 35 Cl–N 2 system [Guinet et al., J Quant Spectrosc Radiat Transfer 2012;113:1113], higher active-gas pressures are reached, providing better signal-to-noise ratios, and the exact expression of the Beer–Lambert law is introduced in the fitting procedure, leading, among other advantages, to much more realistic low-pressure results. The broadening parameters of the considered lines are also computed by a semi-classical method for various relative velocities of colliders and the powers characterizing the dependence of the collisional cross-sections on relative speeds are deduced as functions of the rotational numbers J and K. Additional calculations performed with the Maxwell–Boltzmann distribution of velocities show no significant difference with the earlier results [Buldyreva et al., Phys Chem Chem Phys 2011;13:20326] obtained within the mean thermal velocity approximation. Weighted sums of the presently measured Voigt-profile O 2 -broadening parameters and of the previously published N 2 -broadening ones are calculated to yield experimental air-broadening coefficients for spectroscopic databases. -- Highlights: • Analysis of the speed dependence of relaxation rates of CH 3 Cl lines. • Introduction of the Beer–Lambert law in analysis of line-shapes recorded by FM technique. • Comparison of Maxwell–Boltzmann averaging and mean thermal velocity calculations. • Estimation of air-induced broadening for CH 3 Cl rotational lines

  20. Probing the CO and methanol snow lines in young protostars. Results from the CALYPSO IRAM-PdBI survey

    Science.gov (United States)

    Anderl, S.; Maret, S.; Cabrit, S.; Belloche, A.; Maury, A. J.; André, Ph.; Codella, C.; Bacmann, A.; Bontemps, S.; Podio, L.; Gueth, F.; Bergin, E.

    2016-06-01

    Context. So-called snow lines, indicating regions where abundant volatiles freeze out onto the surface of dust grains, play an important role for planet growth and bulk composition in protoplanetary disks. They can already be observed in the envelopes of the much younger, low-mass Class 0 protostars, which are still in their early phase of heavy accretion. Aims: We aim to use the information on the sublimation regions of different kinds of ices to understand the chemistry of the envelope, its temperature and density structure, and the history of the accretion process. This information is crucial to get the full picture of the early protostellar collapse and the subsequent evolution of young protostars. Methods: As part of the CALYPSO IRAM Large Program, we have obtained observations of C18O, N2H+, and CH3OH towards nearby Class 0 protostars with the IRAM Plateau de Bure interferometer at sub-arcsecond resolution. For four of these sources, we have modeled the emission using a chemical code coupled with a radiative transfer module. Results: We observe an anti-correlation of C18O and N2H+ in NGC 1333-IRAS4A, NGC 1333-IRAS4B, L1157, and L1448C, with N2H+ forming a ring (perturbed by the outflow) around the centrally peaked C18O emission. This emission morphology, which is due to N2H+ being chemically destroyed by CO, reveals the CO and N2 ice sublimation regions in these protostellar envelopes with unprecedented resolution. We also observe compact methanol emission towards three of the sources. Based on our chemical model and assuming temperature and density profiles from the literature, we find that for all four sources the CO snow line appears further inwards than expected from the binding energy of pure CO ices (~855 K). The emission regions of models and observations match for a higher value of the CO binding energy of 1200 K, corresponding to a dust temperature of ~24 K at the CO snow line. The binding energy for N2 ices is modeled at 1000 K, also higher than for

  1. Resonant line transfer in a fog: using Lyman-alpha to probe tiny structures in atomic gas

    Science.gov (United States)

    Gronke, Max; Dijkstra, Mark; McCourt, Michael; Peng Oh, S.

    2017-11-01

    Motivated by observational and theoretical work that suggest very small-scale (≲ 1 pc) structure in the circumgalactic medium of galaxies and in other environments, we study Lyman-α (Lyα) radiative transfer in an extremely clumpy medium with many clouds of neutral gas along the line of sight. While previous studies have typically considered radiative transfer through sightlines intercepting ≲ 10 clumps, we explored the limit of a very large number of clumps per sightline (up to fc 1000). Our main finding is that, for covering factors greater than some critical threshold, a multiphase medium behaves similarly to a homogeneous medium in terms of the emergent Lyα spectrum. The value of this threshold depends on both the clump column density and the movement of the clumps. We estimated this threshold analytically and compare our findings to radiative transfer simulations with a range of covering factors, clump column densities, radii, and motions. Our results suggest that (I) the success in fitting observed Lyα spectra using homogeneous "shell models" (and the corresponding failure of multiphase models) hints at the presence of very small-scale structure in neutral gas, which is in agreement within a number of other observations; and (II) the recurrent problems of reproducing realistic line profiles from hydrodynamical simulations may be due to their inability to resolve small-scale structure, which causes simulations to underestimate the effective covering factor of neutral gas clouds. The movie associated to Fig. B.2 is available at http://www.aanda.org

  2. Interrogating Seyferts with NebulaBayes: Spatially Probing the Narrow-line Region Radiation Fields and Chemical Abundances

    Science.gov (United States)

    Thomas, Adam D.; Dopita, Michael A.; Kewley, Lisa J.; Groves, Brent A.; Sutherland, Ralph S.; Hopkins, Andrew M.; Blanc, Guillermo A.

    2018-04-01

    NebulaBayes is a new Bayesian code that implements a general method of comparing observed emission-line fluxes to photoionization model grids. The code enables us to extract robust, spatially resolved measurements of abundances in the extended narrow-line regions (ENLRs) produced by Active Galactic Nuclei (AGN). We observe near-constant ionization parameters but steeply radially declining pressures, which together imply that radiation pressure regulates the ENLR density structure on large scales. Our sample includes four “pure Seyfert” galaxies from the S7 survey that have extensive ENLRs. NGC 2992 shows steep metallicity gradients from the nucleus into the ionization cones. An inverse metallicity gradient is observed in ESO 138-G01, which we attribute to a recent gas inflow or minor merger. A uniformly high metallicity and hard ionizing continuum are inferred across the ENLR of Mrk 573. Our analysis of IC 5063 is likely affected by contamination from shock excitation, which appears to soften the inferred ionizing spectrum. The peak of the ionizing continuum E peak is determined by the nuclear spectrum and the absorbing column between the nucleus and the ionized nebula. We cannot separate variation in this intrinsic E peak from the effects of shock or H II region contamination, but E peak measurements nevertheless give insights into ENLR excitation. We demonstrate the general applicability of NebulaBayes by analyzing a nuclear spectrum from the non-active galaxy NGC 4691 using a H II region grid. The NLR and H II region model grids are provided with NebulaBayes for use by the astronomical community.

  3. New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents.

    Science.gov (United States)

    Li, Guangrong; Wang, Hongjin; Lang, Tao; Li, Jianbo; La, Shixiao; Yang, Ennian; Yang, Zujun

    2016-10-01

    New molecular markers were developed for targeting Thinopyrum intermedium 1St#2 chromosome, and novel FISH probe representing the terminal repeats was produced for identification of Thinopyrum chromosomes. Thinopyrum intermedium has been used as a valuable resource for improving the disease resistance and yield potential of wheat. A wheat-Th. intermedium ssp. trichophorum chromosome 1St#2 substitution and translocation has displayed superior grain protein and wet gluten content. With the aim to develop a number of chromosome 1St#2 specific molecular and cytogenetic markers, a high throughput, low-cost specific-locus amplified fragment sequencing (SLAF-seq) technology was used to compare the sequences between a wheat-Thinopyrum 1St#2 (1D) substitution and the related species Pseudoroegneria spicata (St genome, 2n = 14). A total of 5142 polymorphic fragments were analyzed and 359 different SLAF markers for 1St#2 were predicted. Thirty-seven specific molecular markers were validated by PCR from 50 randomly selected SLAFs. Meanwhile, the distribution of transposable elements (TEs) at the family level between wheat and St genomes was compared using the SLAFs. A new oligo-nucleotide probe named Oligo-pSt122 from high SLAF reads was produced for fluorescence in situ hybridization (FISH), and was observed to hybridize to the terminal region of 1St#L and also onto the terminal heterochromatic region of Th. intermedium genomes. The genome-wide markers and repetitive based probe Oligo-pSt122 will be valuable for identifying Thinopyrum chromosome segments in wheat backgrounds.

  4. The use of radionuclide DNA probe technology in epidemiological studies of leishmaniasis

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Antero Silva Ribeiro de [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte, MG (Brazil); Fernandes, Octavio [Fundacao Inst. Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, RJ (Brazil). Dept. de Medicina Tropical; Gomes, Rosangela Fatima; Melo, Maria Norma de [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Dept. de Parasitologia]|[Cor Jesus Fontes Mato Grosso Univ., Cuiaba, MT (Brazil). Hospital Universitario Julio Muller

    2000-07-01

    Cutaneous and mucosal leishmaniasis are due to different species that belongs to Leishmania (Leishmania) mexicana complex and Leishmania (Viannia) braziliensis complex, respectively. Cutaneous leishmaniasis ulcers may persist for months to years but eventually they heal, while mucocutaneous leishmaniasis can result in destructive lesions on the nose, oral pharynx, lips or face. The specific diagnosis of the disease is important because of the high cost and toxicity of the treatment and the subsequent medical follow-up depends on the precise identification of the complex that causes the infection. The epidemiological information furnished by the identification of parasites of a given endemic region is also essential for the design of appropriate control measures. In this work we typed, using specific DNA probes labelled with {sup 32}P radionuclide, samples collected from patients living in endemic areas of Mato Grosso state. The results showed that L. braziliensis is the predominant group infecting human patients in the state. We have typed, up to the moment 68 samples. 64 samples (94.1%) belonged to the L. braziliensis complex and only 4 (5.9%) belonged to the L. mexicana complex. (author)

  5. Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures

    Science.gov (United States)

    Zhang, Feng; Gradinaru, Viviana; Adamantidis, Antoine R; Durand, Remy; Airan, Raag D; de Lecea, Luis; Deisseroth, Karl

    2015-01-01

    Elucidation of the neural substrates underlying complex animal behaviors depends on precise activity control tools, as well as compatible readout methods. Recent developments in optogenetics have addressed this need, opening up new possibilities for systems neuroscience. Interrogation of even deep neural circuits can be conducted by directly probing the necessity and sufficiency of defined circuit elements with millisecond-scale, cell type-specific optical perturbations, coupled with suitable readouts such as electrophysiology, optical circuit dynamics measures and freely moving behavior in mammals. Here we collect in detail our strategies for delivering microbial opsin genes to deep mammalian brain structures in vivo, along with protocols for integrating the resulting optical control with compatible readouts (electrophysiological, optical and behavioral). The procedures described here, from initial virus preparation to systems-level functional readout, can be completed within 4–5 weeks. Together, these methods may help in providing circuit-level insight into the dynamics underlying complex mammalian behaviors in health and disease. PMID:20203662

  6. Heat and Mass Transfer Remote Control in Bioreactors of Technological Lines

    Directory of Open Access Journals (Sweden)

    Viktorija M. Mel’nick

    2017-10-01

    Full Text Available Background. The main problems that arise when using equipment for cultivation are to ensure the heat and mass transfer processes in devices, presence of turbulent and stagnant zones, high-energy consumption, low heat transfer coefficients when working with viscous fluids. Objective. The aim of the paper is the experimental determination of the remote control heat transfer advantages in production line bioreactors using ultrasonic beam compared to contact methods. Methods. An experimental study of the heat and mass transfer process in a bioreactor on the stand with UZP-6-1 immersion unit of the ultrasonic radiator with radiation frequency 42 kHz is carried out. Results. Sound waves emitted into a liquid form a concentration zone of passable sound energy in the confocal vessel form of a cylindrical surface and force the liquid to move along the inner surface of the glass along the ascending cylindrical spiral, forming a motive flow throughout the volume, causing peripheral layers of liquid and bottom layers to move in a horizontal and vertical planes, without leaving stagnant zones. The closer to the coincidence angle is the directed ultrasonic beam the greater is the effectiveness of the driving flow. Conclusions. The use of sound waves allows obtaining a high-quality product in technological lines based on bioreactors with minimal risk for the technological process. Radiation parameters and working volume physic-mechanical properties change allow fully using the properties of resonant manifestations of the sound wave influence on the working liquid with minimal costs.

  7. In situ probing of temperature in radio frequency thermal plasma using Yttrium ion emission lines during synthesis of yttria nanoparticles

    Science.gov (United States)

    Dhamale, G. D.; Tiwari, N.; Mathe, V. L.; Bhoraskar, S. V.; Ghorui, S.

    2017-07-01

    Particle feeding is used in the most important applications of radio frequency (r.f.) thermal plasmas like synthesis of nanoparticles and particle spheroidization. The study reports an in-situ investigation of radial distribution of temperature in such devices using yttrium ion emission lines under different rates of particle loading during synthesis of yttria nanoparticles. A number of interesting facts about the response of r.f. plasma to the rate of particle loading, hitherto unknown, are revealed. Observed phenomena are supported with experimental data from fast photographic experiments and actual synthesis results. The use of the Abel inversion technique together with simultaneous multi-track acquisition of emission spectra from different spatial locations using a CCD based spectrometer allowed us to extract accurate distribution of temperature inside the plasma in the presence of inherent instabilities. The temperature profiles of this type of plasma have been measured possibly for the first time while particles are being fed into the plasma. Observed changes in the temperature profiles as the particle feed rate increases are very significant. Reaction forces resulting from particle evaporation, and increased skin depth owing to the decrease in electrical conductivity in the edge region are proposed as the two different mechanisms to account for the observed changes in the temperature profile as the powder feed rate is increased. Quantitative analyses supporting the proposed mechanisms are presented.

  8. Performance comparison of digital microRNA profiling technologies applied on human breast cancer cell lines.

    Directory of Open Access Journals (Sweden)

    Erik Knutsen

    Full Text Available MicroRNA profiling represents an important first-step in deducting individual RNA-based regulatory function in a cell, tissue, or at a specific developmental stage. Currently there are several different platforms to choose from in order to make the initial miRNA profiles. In this study we investigate recently developed digital microRNA high-throughput technologies. Four different platforms were compared including next generation SOLiD ligation sequencing and Illumina HiSeq sequencing, hybridization-based NanoString nCounter, and miRCURY locked nucleic acid RT-qPCR. For all four technologies, full microRNA profiles were generated from human cell lines that represent noninvasive and invasive tumorigenic breast cancer. This study reports the correlation between platforms, as well as a more extensive analysis of the accuracy and sensitivity of data generated when using different platforms and important consideration when verifying results by the use of additional technologies. We found all the platforms to be highly capable for microRNA analysis. Furthermore, the two NGS platforms and RT-qPCR all have equally high sensitivity, and the fold change accuracy is independent of individual miRNA concentration for NGS and RT-qPCR. Based on these findings we propose new guidelines and considerations when performing microRNA profiling.

  9. Emulsion stability measurements by single electrode capacitance probe (SeCaP) technology

    International Nuclear Information System (INIS)

    Schüller, R B; Løkra, S; Egelandsdal, B; Salas-Bringas, C; Engebretsen, B

    2008-01-01

    This paper describes a new and novel method for the determination of the stability of emulsions. The method is based on the single electrode capacitance technology (SeCaP). A measuring system consisting of eight individual measuring cells, each with a volume of approximately 10 ml, is described in detail. The system has been tested on an emulsion system based on whey proteins (WPC80), oil and water. Xanthan was added to modify the emulsion stability. The results show that the new measuring system is able to quantify the stability of the emulsion in terms of a differential variable. The whole separation process is observed much faster in the SeCaP system than in a conventional separation column. The complete separation process observed visually over 30 h is seen in less than 1.4 h in the SeCaP system

  10. Advanced technologies for manufacturing high strength sour grade UOE line pipe

    Energy Technology Data Exchange (ETDEWEB)

    Kobayashi, Kenji; Omura, Tomohiko; Takahashi, Nobuaki; Minato, Izuru; Yamamoto, Akio [Sumitomo Metal Industries, Ltd., Kashima, (Japan)

    2010-07-01

    A new kind of high strength pipeline has been manufactured for sour service in offshore pipelines. This paper first presents a review of developments in manufacturing technology to improve sour resistance. This was particularly the case with Grade UOE line pipe. The improvement was achieved by optimizing the continuous casting process, monitoring the shape of inclusions (such as MnS, CaS, Al2O3, CaO-Al2O3) and decreasing coarse precipitates (Nb(C,N), TiN). The study then used the HIC evaluation method to determine hydrogen induced cracking (HIC) resistance of the material and HAZ test for sulfide stress cracking (SSC) resistance. The evaluation of the NACE TM0284 solution A showed that these pipelines are able to resist severe sour conditions because of good HIC and SSC resistance. Optimizing others components like alloying elements and the ACC process would improve sour resistance in future applications.

  11. Carrier Current Line Systems Technologies in M2M Architecture for Wireless Communication

    Directory of Open Access Journals (Sweden)

    Hua-Ching Chen

    2016-01-01

    Full Text Available This paper investigates the Carrier Current Line Systems (CCLS technologies of Machine to Machine (M2M architecture which applied for mobile station coverage working with metro, high speed railway, and subway such as analysis for public transport of an indoor transition system. It is based on the theory and practical engineering principle which provide guidelines and formulas for link budget design to help designers fully control and analyze the single output power of uplink and downlink between Fiber Repeaters (FR and mobile station as well as base station. Finally, the results of this leakage cable system are successfully applied to indoor coverage design for metro rapid transit system which are easily installed cellular over fiber solutions for WCDMA/LTE access is becoming Ubiquitous Network to Internet of Thing (IOT real case hierarchy of telecommunication.

  12. High-throughput platform assay technology for the discovery of pre-microrna-selective small molecule probes.

    Science.gov (United States)

    Lorenz, Daniel A; Song, James M; Garner, Amanda L

    2015-01-21

    MicroRNAs (miRNA) play critical roles in human development and disease. As such, the targeting of miRNAs is considered attractive as a novel therapeutic strategy. A major bottleneck toward this goal, however, has been the identification of small molecule probes that are specific for select RNAs and methods that will facilitate such discovery efforts. Using pre-microRNAs as proof-of-concept, herein we report a conceptually new and innovative approach for assaying RNA-small molecule interactions. Through this platform assay technology, which we term catalytic enzyme-linked click chemistry assay or cat-ELCCA, we have designed a method that can be implemented in high throughput, is virtually free of false readouts, and is general for all nucleic acids. Through cat-ELCCA, we envision the discovery of selective small molecule ligands for disease-relevant miRNAs to promote the field of RNA-targeted drug discovery and further our understanding of the role of miRNAs in cellular biology.

  13. Rapid and Sensitive Detection of Breast Cancer Cells in Patient Blood with Nuclease-Activated Probe Technology

    Directory of Open Access Journals (Sweden)

    Sven Kruspe

    2017-09-01

    Full Text Available A challenge for circulating tumor cell (CTC-based diagnostics is the development of simple and inexpensive methods that reliably detect the diverse cells that make up CTCs. CTC-derived nucleases are one category of proteins that could be exploited to meet this challenge. Advantages of nucleases as CTC biomarkers include: (1 their elevated expression in many cancer cells, including cells implicated in metastasis that have undergone epithelial-to-mesenchymal transition; and (2 their enzymatic activity, which can be exploited for signal amplification in detection methods. Here, we describe a diagnostic assay based on quenched fluorescent nucleic acid probes that detect breast cancer CTCs via their nuclease activity. This assay exhibited robust performance in distinguishing breast cancer patients from healthy controls, and it is rapid, inexpensive, and easy to implement in most clinical labs. Given its broad applicability, this technology has the potential to have a substantive impact on the diagnosis and treatment of many cancers. Keywords: cancer, circulating tumor cells, diagnostic nucleic acids, nucleases, diagnostic markers, breast cancer, liquid biopsy

  14. Mobile Probing and Probes

    DEFF Research Database (Denmark)

    Duvaa, Uffe; Ørngreen, Rikke; Weinkouff Mathiasen, Anne-Gitte

    2013-01-01

    Mobile probing is a method, developed for learning about digital work situations, as an approach to discover new grounds. The method can be used when there is a need to know more about users and their work with certain tasks, but where users at the same time are distributed (in time and space......). Mobile probing was inspired by the cultural probe method, and was influenced by qualitative interview and inquiry approaches. The method has been used in two subsequent projects, involving school children (young adults at 15-17 years old) and employees (adults) in a consultancy company. Findings point...... to mobile probing being a flexible method for uncovering the unknowns, as a way of getting rich data to the analysis and design phases. On the other hand it is difficult to engage users to give in depth explanations, which seem easier in synchronous dialogs (whether online or face2face). The development...

  15. Mobile Probing and Probes

    DEFF Research Database (Denmark)

    Duvaa, Uffe; Ørngreen, Rikke; Weinkouff, Anne-Gitte

    2012-01-01

    Mobile probing is a method, which has been developed for learning about digital work situations, as an approach to discover new grounds. The method can be used when there is a need to know more about users and their work with certain tasks, but where users at the same time are distributed (in time...... and space). Mobile probing was inspired by the cultural probe method, and was influenced by qualitative interview and inquiry approaches. The method has been used in two subsequent projects, involving school children (young adults at 15-17 years old) and employees (adults) in a consultancy company. Findings...... point to mobile probing being a flexible method for uncovering the unknowns, as a way of getting rich data to the analysis and design phases. On the other hand it is difficult to engage users to give in depth explanations, which seem easier in synchronous dialogs (whether online or face2face...

  16. The use of 'Omics technology to rationally improve industrial mammalian cell line performance.

    Science.gov (United States)

    Lewis, Amanda M; Abu-Absi, Nicholas R; Borys, Michael C; Li, Zheng Jian

    2016-01-01

    Biologics represent an increasingly important class of therapeutics, with 7 of the 10 top selling drugs from 2013 being in this class. Furthermore, health authority approval of biologics in the immuno-oncology space is expected to transform treatment of patients with debilitating and deadly diseases. The growing importance of biologics in the healthcare field has also resulted in the recent approvals of several biosimilars. These recent developments, combined with pressure to provide treatments at lower costs to payers, are resulting in increasing need for the industry to quickly and efficiently develop high yielding, robust processes for the manufacture of biologics with the ability to control quality attributes within narrow distributions. Achieving this level of manufacturing efficiency and the ability to design processes capable of regulating growth, death and other cellular pathways through manipulation of media, feeding strategies, and other process parameters will undoubtedly be facilitated through systems biology tools generated in academic and public research communities. Here we discuss the intersection of systems biology, 'Omics technologies, and mammalian bioprocess sciences. Specifically, we address how these methods in conjunction with traditional monitoring techniques represent a unique opportunity to better characterize and understand host cell culture state, shift from an empirical to rational approach to process development and optimization of bioreactor cultivation processes. We summarize the following six key areas: (i) research applied to parental, non-recombinant cell lines; (ii) systems level datasets generated with recombinant cell lines; (iii) datasets linking phenotypic traits to relevant biomarkers; (iv) data depositories and bioinformatics tools; (v) in silico model development, and (vi) examples where these approaches have been used to rationally improve cellular processes. We critically assess relevant and state of the art research

  17. On-line monitoring system of PV array based on internet of things technology

    Science.gov (United States)

    Li, Y. F.; Lin, P. J.; Zhou, H. F.; Chen, Z. C.; Wu, L. J.; Cheng, S. Y.; Su, F. P.

    2017-11-01

    The Internet of Things (IoT) Technology is used to inspect photovoltaic (PV) array which can greatly improve the monitoring, performance and maintenance of the PV array. In order to efficiently realize the remote monitoring of PV operating environment, an on-line monitoring system of PV array based on IoT is designed in this paper. The system includes data acquisition, data gateway and PV monitoring centre (PVMC) website. Firstly, the DSP-TMS320F28335 is applied to collect indicators of PV array using sensors, then the data are transmitted to data gateway through ZigBee network. Secondly, the data gateway receives the data from data acquisition part, obtains geographic information via GPS module, and captures the scenes around PV array via USB camera, then uploads them to PVMC website. Finally, the PVMC website based on Laravel framework receives all data from data gateway and displays them with abundant charts. Moreover, a fault diagnosis approach for PV array based on Extreme Learning Machine (ELM) is applied in PVMC. Once fault occurs, a user alert can be sent via E-mail. The designed system enables users to browse the operating conditions of PV array on PVMC website, including electrical, environmental parameters and video. Experimental results show that the presented monitoring system can efficiently real-time monitor the PV array, and the fault diagnosis approach reaches a high accuracy of 97.5%.

  18. On-line Versus Face-to-Face Education: Utilizing Technology to Increase Effectiveness

    Science.gov (United States)

    2012-05-17

    Andragogy and learner-centered education provided in on-line education present excellent results. Secondly, the cost of on-line education compared to...13 Pedagogy versus Andragogy ...achieving learning outcomes. However, Andragogy and learner centered education provided in on-line education presents excellent results. Secondly, the cost

  19. 银镜生产线操作工艺%Production Operating Technology of Silver-mirror Line

    Institute of Scientific and Technical Information of China (English)

    徐卫民; 杨晨; 梁中生

    2011-01-01

    详细介绍了银镜生产线的实际操作工艺,阐述了每道工序的注意事项。%The practical operating technology of silver-mirror production line was described in details and the matters need attention at every stage were also presented.

  20. [Applications and prospects of on-line near infrared spectroscopy technology in manufacturing of Chinese materia medica].

    Science.gov (United States)

    Li, Yang; Wu, Zhi-Sheng; Pan, Xiao-Ning; Shi, Xin-Yuan; Guo, Ming-Ye; Xu, Bing; Qiao, Yan-Jiang

    2014-10-01

    The quality of Chinese materia medica (CMM) is affected by every process in CMM manufacturing. According to multi-unit complex features in the production of CMM, on-line near infrared spectroscopy (NIR) is used as an evaluating technology with its rapid, non-destructive and non-pollution etc. advantages. With the research in institutions, the on-line NIR applied in process analysis and control of CMM was described systematically, and the on-line NIR platform building was used as an example to clarify the feasibility of on-line NIR technology in CMM manufacturing process. Then, from the point of application by pharmaceutical companies, the current on-line NIR research on CMM and its production in pharmaceutical companies was relatively comprehensively summarized. Meanwhile, the types of CMM productions were classified in accordance with two formulations (liquid and solid dosage formulations). The different production processes (extraction, concentration and alcohol precipitation, etc. ) were used as liquid formulation diacritical points; the different types (tablets, capsules and plasters, etc.) were used as solid dosage formulation diacritical points, and the reliability of on-line NIR used in the whole process in CMM production was proved in according to the summary of literatures in recent 10 years, which could support the modernization of CMM production.

  1. Establishing a store baseline during interim storage of waste packages and a review of potential technologies for base-lining

    Energy Technology Data Exchange (ETDEWEB)

    McTeer, Jennifer; Morris, Jenny; Wickham, Stephen [Galson Sciences Ltd. Oakham, Rutland (United Kingdom); Bolton, Gary [National Nuclear Laboratory Risley, Warrington (United Kingdom); McKinney, James; Morris, Darrell [Nuclear Decommissioning Authority Moor Row, Cumbria (United Kingdom); Angus, Mike [National Nuclear Laboratory Risley, Warrington (United Kingdom); Cann, Gavin; Binks, Tracey [National Nuclear Laboratory Sellafield (United Kingdom)

    2013-07-01

    Interim storage is an essential component of the waste management lifecycle, providing a safe, secure environment for waste packages awaiting final disposal. In order to be able to monitor and detect change or degradation of the waste packages, storage building or equipment, it is necessary to know the original condition of these components (the 'waste storage system'). This paper presents an approach to establishing the baseline for a waste-storage system, and provides guidance on the selection and implementation of potential base-lining technologies. The approach is made up of two sections; assessment of base-lining needs and definition of base-lining approach. During the assessment of base-lining needs a review of available monitoring data and store/package records should be undertaken (if the store is operational). Evolutionary processes (affecting safety functions), and their corresponding indicators, that can be measured to provide a baseline for the waste-storage system should then be identified in order for the most suitable indicators to be selected for base-lining. In defining the approach, identification of opportunities to collect data and constraints is undertaken before selecting the techniques for base-lining and developing a base-lining plan. Base-lining data may be used to establish that the state of the packages is consistent with the waste acceptance criteria for the storage facility and to support the interpretation of monitoring and inspection data collected during store operations. Opportunities and constraints are identified for different store and package types. Technologies that could potentially be used to measure baseline indicators are also reviewed. (authors)

  2. Development and application of gamma scanning technology for on-line investigation of industrial process columns and vessels

    International Nuclear Information System (INIS)

    Jaafar Abdullah

    1999-01-01

    Plant Assessment Technology (PAT) group, in association with Intelligent System (IS) Group and Engineering Services Department of Malaysian Institute for Nuclear Technology Research (MINT) has developed gamma scanning facilities for on-line investigation of industrial process columns and vessels. The technology, based on the principle of gamma-ray absorption, has been successfully applied for troubleshooting of a number of distillation columns and process vessels in petroleum refineries, gas processing plants and chemical plants in the country and the region. This paper outlines basic characteristics of the system and describes the inspection procedures, and in addition, case studies are also presented. The case studies are purposely chosen to illustrate the versatility of the technology, and furthermore to demonstrate the economic benefits which can be realised from the application of this technology. (author)

  3. The Impact of a Line Probe Assay Based Diagnostic Algorithm on Time to Treatment Initiation and Treatment Outcomes for Multidrug Resistant TB Patients in Arkhangelsk Region, Russia.

    Science.gov (United States)

    Eliseev, Platon; Balantcev, Grigory; Nikishova, Elena; Gaida, Anastasia; Bogdanova, Elena; Enarson, Donald; Ornstein, Tara; Detjen, Anne; Dacombe, Russell; Gospodarevskaya, Elena; Phillips, Patrick P J; Mann, Gillian; Squire, Stephen Bertel; Mariandyshev, Andrei

    2016-01-01

    In the Arkhangelsk region of Northern Russia, multidrug-resistant (MDR) tuberculosis (TB) rates in new cases are amongst the highest in the world. In 2014, MDR-TB rates reached 31.7% among new cases and 56.9% among retreatment cases. The development of new diagnostic tools allows for faster detection of both TB and MDR-TB and should lead to reduced transmission by earlier initiation of anti-TB therapy. The PROVE-IT (Policy Relevant Outcomes from Validating Evidence on Impact) Russia study aimed to assess the impact of the implementation of line probe assay (LPA) as part of an LPA-based diagnostic algorithm for patients with presumptive MDR-TB focusing on time to treatment initiation with time from first-care seeking visit to the initiation of MDR-TB treatment rather than diagnostic accuracy as the primary outcome, and to assess treatment outcomes. We hypothesized that the implementation of LPA would result in faster time to treatment initiation and better treatment outcomes. A culture-based diagnostic algorithm used prior to LPA implementation was compared to an LPA-based algorithm that replaced BacTAlert and Löwenstein Jensen (LJ) for drug sensitivity testing. A total of 295 MDR-TB patients were included in the study, 163 diagnosed with the culture-based algorithm, 132 with the LPA-based algorithm. Among smear positive patients, the implementation of the LPA-based algorithm was associated with a median decrease in time to MDR-TB treatment initiation of 50 and 66 days compared to the culture-based algorithm (BacTAlert and LJ respectively, ptime to MDR-TB treatment initiation of 78 days when compared to the culture-based algorithm (LJ, ptime to MDR diagnosis and earlier treatment initiation as well as better treatment outcomes for patients with MDR-TB. These findings also highlight the need for further improvements within the health system to reduce both patient and diagnostic delays to truly optimize the impact of new, rapid diagnostics.

  4. Hepatitis C virus Genie: A web 2.0 interpretation and analytics platform for the Versant Hepatitis C virus genotype Line Probe Assay version 2.0

    Directory of Open Access Journals (Sweden)

    Alex M Dussaq

    2017-01-01

    Full Text Available Context: Hepatitis C virus (HCV genotyping at our institution is performed using the Versant HCV genotype 2.0 Line Probe Assay (LiPA. The last steps of this procedure are manual, laborious, and error-prone process that involves the comparison of the banding pattern on a test strip to a physical reference table. Aim: We developed a web-based HCV genotype interpretation platform that utilizes a scanned image to generate the genotypes, thus minimizing interpretation time and reducing error. Subjects and Methods: HCV Genie 2 utilizes a database of banding patterns in conjuncture with image analysis algorithms to determine the genotype for any number of scanned LiPA strips. HCV Genie 2 is built with client-side JavaScript; allowing the program to run in the user' browser rather than on an unknown server, essentially eliminating data and patient privacy concerns. Results: HCV Genie 2 was tested over 2 months and proved identical to human expert interpretation for 148 samples (>1000 bands identified. Manual intervention was required only for two faint bands and one false-positive band; this was done utilizing the built-in-user interface. Utilizing the original method, the trained laboratory technician interpretation time for 16 samples was 13.8 (±0.96 min as compared to 5.0 (±1.09 min with HCV Genie 2, a 63.8% decrease. In addition to the time savings, the new method provides an additional validation step, which decreases the potential for errors. Conclusions: Our institution has moved exclusively to utilize the new techniques and tools described here. Both experienced technicians and the molecular pathologists at our institution prefer the workflow using HCV Genie. It is easier for the technicians to prepare and document, and the pathologists are more rapidly able to review and confirm results. The use of this tool will lead to increase the quality of patient care delivered through this test methodology by decreasing the potential for error. The

  5. Hepatitis C Virus Genie: A Web 2.0 Interpretation and Analytics Platform for the Versant Hepatitis C Virus Genotype Line Probe Assay Version 2.0.

    Science.gov (United States)

    Dussaq, Alex M; Soni, Abha; Willey, Christopher; Park, Seung L; Harada, Shuko

    2017-01-01

    Hepatitis C virus (HCV) genotyping at our institution is performed using the Versant HCV genotype 2.0 Line Probe Assay (LiPA). The last steps of this procedure are manual, laborious, and error-prone process that involves the comparison of the banding pattern on a test strip to a physical reference table. We developed a web-based HCV genotype interpretation platform that utilizes a scanned image to generate the genotypes, thus minimizing interpretation time and reducing error. HCV Genie 2 utilizes a database of banding patterns in conjuncture with image analysis algorithms to determine the genotype for any number of scanned LiPA strips. HCV Genie 2 is built with client-side JavaScript; allowing the program to run in the user' browser rather than on an unknown server, essentially eliminating data and patient privacy concerns. HCV Genie 2 was tested over 2 months and proved identical to human expert interpretation for 148 samples (>1000 bands identified). Manual intervention was required only for two faint bands and one false-positive band; this was done utilizing the built-in-user interface. Utilizing the original method, the trained laboratory technician interpretation time for 16 samples was 13.8 (±0.96) min as compared to 5.0 (±1.09) min with HCV Genie 2, a 63.8% decrease. In addition to the time savings, the new method provides an additional validation step, which decreases the potential for errors. Our institution has moved exclusively to utilize the new techniques and tools described here. Both experienced technicians and the molecular pathologists at our institution prefer the workflow using HCV Genie. It is easier for the technicians to prepare and document, and the pathologists are more rapidly able to review and confirm results. The use of this tool will lead to increase the quality of patient care delivered through this test methodology by decreasing the potential for error. The algorithms developed here can be ported to similar band identification

  6. On-Line Ion Exchange Liquid Chromatography as a Process Analytical Technology for Monoclonal Antibody Characterization in Continuous Bioprocessing.

    Science.gov (United States)

    Patel, Bhumit A; Pinto, Nuno D S; Gospodarek, Adrian; Kilgore, Bruce; Goswami, Kudrat; Napoli, William N; Desai, Jayesh; Heo, Jun H; Panzera, Dominick; Pollard, David; Richardson, Daisy; Brower, Mark; Richardson, Douglas D

    2017-11-07

    Combining process analytical technology (PAT) with continuous production provides a powerful tool to observe and control monoclonal antibody (mAb) fermentation and purification processes. This work demonstrates on-line liquid chromatography (on-line LC) as a PAT tool for monitoring a continuous biologics process and forced degradation studies. Specifically, this work focused on ion exchange chromatography (IEX), which is a critical separation technique to detect charge variants. Product-related impurities, including charge variants, that impact function are classified as critical quality attributes (CQAs). First, we confirmed no significant differences were observed in the charge heterogeneity profile of a mAb through both at-line and on-line sampling and that the on-line method has the ability to rapidly detect changes in protein quality over time. The robustness and versatility of the PAT methods were tested by sampling from two purification locations in a continuous mAb process. The PAT IEX methods used with on-line LC were a weak cation exchange (WCX) separation and a newly developed shorter strong cation exchange (SCX) assay. Both methods provided similar results with the distribution of percent acidic, main, and basic species remaining unchanged over a 2 week period. Second, a forced degradation study showed an increase in acidic species and a decrease in basic species when sampled on-line over 7 days. These applications further strengthen the use of on-line LC to monitor CQAs of a mAb continuously with various PAT IEX analytical methods. Implementation of on-line IEX will enable faster decision making during process development and could potentially be applied to control in biomanufacturing.

  7. Strain distribution analysis in Si/SiGe line structures for CMOS technology using Raman spectroscopy

    International Nuclear Information System (INIS)

    Hecker, M; Roelke, M; Hermann, P; Zschech, E; Vartanian, V

    2010-01-01

    Strained silicon underneath the field-effect transistor gate increases significantly the charge carrier mobility and thus improves the performance of leading-edge Complementary Metal Oxide Semiconductor (CMOS) devices. For better understanding of the structure-strain relationship on the nanoscale and for optimization of device structures, the measurement of the local strain state has become essential. Raman spectroscopy is used in the present investigation to analyze the strain distribution in and close to silicon/embedded silicon-germanium (SiGe) line structures in conjunction with strain modeling applying finite element analysis. Both experimental results and modeling indicate the impact of geometry on the stress state. An increase of compressive stress within the Si lines is obtained for increasing SiGe line widths and decreasing Si line widths. The stress state within the Si lines is shown to be a mixed one deviating from a pure uniaxial state. Underneath the SiGe cavities, the presence of a tensile stress was observed. To investigate a procedure to scale down the spatial resolution of the Raman measurements, tip-enhanced Raman scattering experiments have been performed on free-standing SiGe lines with 100nm line width and line distance. The results show superior resolution and strain information not attainable in conventional Raman scans.

  8. Development of plasma diagnostics technologies - Measurement of transport= parameters in tokamak edge plasma by using electric transport probes

    Energy Technology Data Exchange (ETDEWEB)

    Chung, Kyu Sun; Chang, Do Hee; Sim, Yeon Gun; Kim, Jin Hee [Hanyang University, Seoul (Korea, Republic of)

    1995-08-01

    Electric transport probe system is developed for the measurement of electron temperature, floating potential, plasma density and flow velocity of= edge plasmas in the KT-2 medium size tokamak. Experiments have been performed in KT-1 small size tokamak. Electric transport probe is composed of a single probe(SP) and a Mach probe (MP). SP is used for the measurements of electron density, floating potential, and plasma density and measured values are {approx} 3*10{sup 11}/cm{sup -3}, -20 volts, 15 {approx} 25 eV. For the most discharges, respectively. MP is for the measurements of toroidal(M{sub T}) and poloidal(M{sub P}) flow velocities, and density, which are M{sub T} {approx_equal} .0.85, M{sub P} {approx_equal}. 0.17, n. {approx_equal} 2.1*10{sup 11} cm{sup -3}, respectively. A triple probe is also developed for the direct reading of T{sub e} and n{sub e}, and is used for DC, RF, and RF+DC plasma in APL of Hanyang university. 38 refs., 36 figs. (author)

  9. Continuous-flow liquid microjunction surface sampling probe connected on-line with high-performance liquid chromatography/mass spectrometry for spatially resolved analysis of small molecules and proteins.

    Science.gov (United States)

    Van Berkel, Gary J; Kertesz, Vilmos

    2013-06-30

    A continuous-flow liquid microjunction surface sampling probe extracts soluble material from surfaces for direct ionization and detection by mass spectrometry. Demonstrated here is the on-line coupling of such a probe with high-performance liquid chromatography/mass spectrometry (HPLC/MS) enabling extraction, separation and detection of small molecules and proteins from surfaces in a spatially resolved (~0.5 mm diameter spots) manner. A continuous-flow liquid microjunction surface sampling probe was connected to a six-port, two-position valve for extract collection and injection to an HPLC column. A QTRAP® 5500 hybrid triple quadrupole linear ion trap equipped with a Turbo V™ ion source operated in positive electrospray ionization (ESI) mode was used for all experiments. The system operation was tested with the extraction, separation and detection of propranolol and associated metabolites from drug dosed tissues, caffeine from a coffee bean, cocaine from paper currency, and proteins from dried sheep blood spots on paper. Confirmed in the tissue were the parent drug and two different hydroxypropranolol glucuronides. The mass spectrometric response for these compounds from different locations in the liver showed an increase with increasing extraction time (5, 20 and 40 s). For on-line separation and detection/identification of extracted proteins from dried sheep blood spots, two major protein peaks dominated the chromatogram and could be correlated with the expected masses for the hemoglobin α and β chains. Spatially resolved sampling, separation, and detection of small molecules and proteins from surfaces can be accomplished using a continuous-flow liquid microjunction surface sampling probe coupled on-line with HPLC/MS detection. Published in 2013. This article is a U.S. Government work and is in the public domain in the USA.

  10. Preliminary study of steep pulse irreversible electroporation technology in human large cell lung cancer cell lines L9981

    Directory of Open Access Journals (Sweden)

    Song Zuoqing

    2013-01-01

    Full Text Available Our aim was to validate the effectiveness of steep pulse irreversible electroporation technology in human large cell lung cancer cells and to screen the optimal treatment of parameters for human large cell lung cancer cells. Three different sets of steep pulse therapy parameters were applied on the lung cancer cell line L9981. The cell line L9981 inhibition rate and proliferation capacity were detected by Vi-Cell vitality analysis and MTT. Steep pulsed irreversible electroporation technology for large cell lung cancer L9981 presents killing effects with various therapy parameters. The optimal treatment parameters are at a voltage amplitude of 2000V/cm, pulse width of 100μs, pulse frequency of 1 Hz, pulse number 10. With this group of parameters, steep pulse could have the best tumor cell-killing effects.

  11. Detection and explore the noise stress by running technology lines of haylage.

    OpenAIRE

    ŠTANGL, Josef

    2009-01-01

    The tendency of this works was measure and explore noise stress by running lines of haylage. It was plumbed noise applied on service of machines, outside natural scene and human residence. Results were be compared with permitted norms.

  12. Elaboration and installation of technology of on-line diagnostics of important equipment damage as a procedure of NPP lifetime management

    International Nuclear Information System (INIS)

    Bakirov, M.; Povarov, V.

    2012-01-01

    In contrast to conventional approaches used for diagnostics (i.e. when inspection results are used as data for numerical calculative strength analysis) the specific feature of the new proposed approach consists in a fact that the approach is based on application of the 'inverse problem' principle. As regards to implementation of the proposed new approach, first of all a detailed numerical calculative finite-element model of the monitored equipment must be developed. Results of preliminary calculations allow to make reasonable selection of definite installation places and types of control sensors intended for more effective and precise work of the calculative model. As a rule, the control sensors are high-temperature strain gauges, temperature probes, pressure, acceleration and displacement sensors, as well as acoustic-emission and ultrasonic sensors used for monitoring of actual defectiveness kinetics in the zone of potential damaging. All the sensors work in the on-line mode during several years of operation, the optimal frequency of data records is selected, all recorded data after prompt processing are transferred to the finite-element calculative modulus for strength calculations of a monitored zone. Software for strength calculation must be based on an individual calculative code, since it should also work in the on-line mode. Comprehensible strength analysis in conjunction with obtained results of defectiveness kinetics monitoring allow not only to foresee the most unfavorable scenario resulting to damaging, but also to have a possibility for prompt analysis and elaboration of compensating measures allowing to reduce operational loadings. In the report the results of development and practical application of the new approach at NPPs and corresponding technology are presented. (author)

  13. The use of radionuclide DNA probe technology for epidemiological studies of tegumentary leishmaniasis in Mato Grosso state, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Antero Silva Ribeiro de [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN), Belo Horizonte, MG (Brazil); Fernandes, Octavio [Fundacao Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, RJ (Brazil). Dept. de Medicina Tropical; Heub, Marcia; Fontes, Cor Jesus [Universidade Federal do Mato Grosso, Cuiaba, MT (Brazil). Hospital Universitario Julio Muller; Carvalho, Maria de Lourdes Ribeiro; Melo, Maria Norma de [Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil). Dept. de Parasitologia

    2005-10-15

    DNA hybridisation, using probes labelled with 32 P, was used to type Leishmania samples isolated from patients living in endemic areas of Mato Grosso State (Brazil), and clinically diagnosed as having tegumentary leishmaniasis. k DNA cloned mini-circle probes specific for the Leishmania mexicana and Leishmania braziliensis complexes were used. The results showed that L. braziliensis is the predominant group infecting human patients in the state. Sixty-eight samples were typed, 64 samples (94.1%) belonging to the L. braziliensis complex and only four (5.9%) belonging to the L. mexicana complex. Accurate identification of the Leishmania permits better orientation of the medical follow-up, since clinical manifestations may vary depending on the complex to which the parasite belongs. The epidemiological information furnished by the identification of the Leishmania in given endemic area is also essential for the design of appropriate control measures. (author)

  14. The use of radionuclide DNA probe technology for epidemiological studies of tegumentary leishmaniasis in Mato Grosso state, Brazil

    International Nuclear Information System (INIS)

    Andrade, Antero Silva Ribeiro de; Fernandes, Octavio; Heub, Marcia; Fontes, Cor Jesus; Carvalho, Maria de Lourdes Ribeiro; Melo, Maria Norma de

    2005-01-01

    DNA hybridisation, using probes labelled with 32 P, was used to type Leishmania samples isolated from patients living in endemic areas of Mato Grosso State (Brazil), and clinically diagnosed as having tegumentary leishmaniasis. k DNA cloned mini-circle probes specific for the Leishmania mexicana and Leishmania braziliensis complexes were used. The results showed that L. braziliensis is the predominant group infecting human patients in the state. Sixty-eight samples were typed, 64 samples (94.1%) belonging to the L. braziliensis complex and only four (5.9%) belonging to the L. mexicana complex. Accurate identification of the Leishmania permits better orientation of the medical follow-up, since clinical manifestations may vary depending on the complex to which the parasite belongs. The epidemiological information furnished by the identification of the Leishmania in given endemic area is also essential for the design of appropriate control measures. (author)

  15. DOUBLE-SHELL TANK WASTE TRANSFER LINE ENCASEMENT INTEGRITY ASSESSMENT TECHNOLOGY STUDY

    International Nuclear Information System (INIS)

    BOWER, R.R.

    2006-01-01

    The report provides various alternative methods of performing integrity assessment inspections of buried Hanford Double Shell Tank waste transfer line encasements, and provides method recommendations as an alternative to costly encasement pneumatic leak testing. A schedule for future encasement integrity assessments is also included

  16. Comparative study of biological and technological characters in three generations of silkworm Bombyx mori L. ameiotic, parthenogenetically cloned lines.

    Science.gov (United States)

    Greiss, H; Vassilieva, J; Petkov, N; Petkov, Z

    2004-11-01

    Detect any deviation in biologic and technologic characters of eight ameiotic-parthenogenetically cloned lines of Bombyx mori L. from different origins from a normal sexually reproduced control line in three generations. Comparative study of the three generations was conducted in SES, Vratza, unit of the National Center for Agrarian Sciences of Bulgaria after fixing all environmental rearing conditions. The ameiotic-parthen-clones displayed good parthenogenetic development, although total hatchability was significantly less than the sexually reproducing control populations. Survival rates between clones and control were not significantly different. All clones displayed significantly longer larval periods. Slight decline in second generation, and a steeper one in the third generation were observed for all eight cloned lines in cocoon weight, shell weight, and shell ratio and these differences were statistically significant. Cocoon yield was significantly lower than the control throughout the three generations. Our parthen-cloning method has a high rate of success in comparison to other cloning methods, the cloned progeny populations although were weaker technologically (cocoon weight, shell weight, and shell ratio), the biological characters (parthenogenetic development and survival rate) were not compromised. Further study is needed to determine the thermal needs of the cloned embryos and metabolic rate of all stages.

  17. Beam line optics technologies series (7). Orthopedic treatment of sharp of light (reflecting mirror)

    International Nuclear Information System (INIS)

    Uruga, Tomoya; Nomura, Masaharu

    2006-01-01

    A reflecting mirror (mirror) is the most popular light device for orthopedic treatment of the shape of light. The paper explains the kinds of mirror for hard X-ray field and its applications in order to think the objects of mirror and how to adjust it when user experiment on the beam-line. The basic knowledge of reflection of X-ray, a use of mirror, the kinds of condenser mirror, the influence factors on the condenser size, arrangement of mirror in the hard X-ray beam-line, what kinds of mirror are necessary, evaluation of performance of mirror and adjustment, and troubles and measures are described. Layout in optics hutch at BL01B1 at SPring-8, refraction and total reflection of X-rays at surface, reflectivity of Rh and Pt with ideal surface as a function of photon energy, effects of surface roughness on reflectivity of Rh, calculated beam sizes for typical SPring-8 mirror as a function of magnification, schematic drawing of mirror, standard mirror system for vertical deflection in bending magnet beam-line, and observed and calculated reflectivity of Rh double mirror at BL01B1 at SPring-8 are illustrated. (S.Y)

  18. The natural abundance of 13C with different agricultural management by NIRS with fibre optic probe technology.

    Science.gov (United States)

    Fuentes, Mariela; González-Martín, Inmaculada; Hernández-Hierro, Jose Miguel; Hidalgo, Claudia; Govaerts, Bram; Etchevers, Jorge; Sayre, Ken D; Dendooven, Luc

    2009-06-30

    In the present study the natural abundance of (13)C is quantified in agricultural soils in Mexico which have been submitted to different agronomic practices, zero and conventional tillage, retention of crop residues (with and without) and rotation of crops (wheat and maize) for 17 years, which have influenced the physical, chemical and biological characteristics of the soil. The natural abundance of C13 is quantified by near infrared spectra (NIRS) with a remote reflectance fibre optic probe, applying the probe directly to the soil samples. Discriminate partial least squares analysis of the near infrared spectra allowed to classify soils with and without residues, regardless of the type of tillage or rotation systems used with a prediction rate of 90% in the internal validation and 94% in the external validation. The NIRS calibration model using a modified partial least squares regression allowed to determine the delta(13)C in soils with or without residues, with multiple correlation coefficients 0.81 and standard error prediction 0.5 per thousand in soils with residues and 0.92 and 0.2 per thousand in soils without residues. The ratio performance deviation for the quantification of delta(13)C in soil was 2.5 in soil with residues and 3.8 without residues. This indicated that the model was adequate to determine the delta(13)C of unknown soils in the -16.2 per thousand to -20.4 per thousand range. The development of the NIR calibration permits analytic determinations of the values of delta(13)C in unknown agricultural soils in less time, employing a non-destructive method, by the application of the fibre optic probe of remote reflectance to the soil sample.

  19. On-line process analysis innovation: DiComp (tm) shunting dielectric sensor technology

    Science.gov (United States)

    Davis, Craig R.; Waldman, Frank A.

    1993-01-01

    The DiComp Shunting Dielectric Sensor (SDS) is a new patent-pending technology developed under the Small Business Innovation Research Program (SBIR) for NASA's Kennedy Space Center. The incorporation of a shunt electrode into a conventional fringing field dielectric sensor makes the SDS uniquely sensitive to changes in material dielectric properties in the KHz to MHz range which were previously detectable only at GHz measurement frequencies. The initial NASA application of the SDS for Nutrient Delivery Control has demonstrated SDS capabilities for thickness and concentration measurement of Hoagland nutrient solutions. The commercial introduction of DiComp SDS technology for concentration and percent solids measurements in dispersions, emulsions and solutions represents a new technology for process measurements for liquids in a variety of industries.

  20. [Development of a service on line advice and information technology management for health].

    Science.gov (United States)

    Berrospi Polo, Victor; Rodriguez Abad, Juan; Bobadilla Aguilar, Juan; Di Liberto Moreno, Carlos; Díaz Arroyo, Cecilia; Rafael Quipan, Carlos

    2015-10-01

    To validate an advisory service and online information technology management for health and helps to make assessment and acquisition processes an informed medical equipment according to the market and the needs of the health institutions. Internet via a technological solution supported ona data base containing systematic and updated information on technical specifications of 25 compared medical equipment, the same reference prices, list of suppliers, agents and / or producers and technical standards are developed. The"virtual" technical assistance was made with the support of a team of specialists in Health Technology Management, the decision makers in the planning, evaluation and procurement of biomedical equipment. The validation of the service was conducted by involving specialists in the field of Health Technology Management, from different disciplines and institutions who worked in health, public and private. They used the service for a period of time to verify its feasibility of use as well as its usefulness for their planning, evaluation and procurement of biomedical equipment. To these experts we applied a survey before and after them about the software developed in this project. We found that it is common to use the internet to search for information on medical equipment.Also,an increase on the view that the application will help in procurement of biomedical equipment(40% to 78%) was observed, it will improve the information system(40% to 89%) and communication among physicians,nurses, planners, engineers and other professionals involved in this process(20% to 78%). There is a need for a technological tool available with such features contribute to technology management in Peru.

  1. Probing the Physics of Narrow-line Regions in Active Galaxies. IV. Full Data Release of the Siding Spring Southern Seyfert Spectroscopic Snapshot Survey (S7)

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, Adam D.; Dopita, Michael A.; Davies, Rebecca; Hampton, Elise; Kewley, Lisa; Banfield, Julie; Groves, Brent; Sutherland, Ralph [RSAA, Australian National University, Cotter Road, Weston Creek, ACT 2611 (Australia); Shastri, Prajval; Sairam, Lalitha [Indian Institute of Astrophysics, Sarjapur Road, Bengaluru 560034 (India); James, Bethan L. [Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD 21218 (United States); Jin, Chichuan [Max-Planck-Institut für Extraterrestrische Physik, Garching (Germany); Juneau, Stéphanie [CEA-Saclay, DSM/IRFU/SAp, F-91191 Gif-sur-Yvette (France); Kharb, Preeti [National Centre for Radio Astrophysics—Tata Institute of Fundamental Research, Pune University Campus, Post Bag 3, Ganeshkhind Pune 411007 (India); Scharwächter, Julia [Gemini Observatory, Northern Operations Center, 670 N. A’ohoku Place, Hilo, Hawaii 96720 (United States); Shalima, P. [Regional Institute of Education, Manasagangotri, Mysore 570006 (India); Sundar, M. N. [Jain University, 3rd Block Jayanagar, Bengaluru 560011 (India); Zaw, Ingyin, E-mail: adam.thomas@anu.edu.au [New York University (Abu Dhabi), 70 Washington Sq. S, New York, NY 10012 (United States)

    2017-09-01

    We present the second and final data release of the Siding Spring Southern Seyfert Spectroscopic Snapshot Survey (S7). Data are presented for 63 new galaxies not included in the first data release, and we provide 2D emission-line fitting products for the full S7 sample of 131 galaxies. The S7 uses the WiFeS instrument on the ANU 2.3 m telescope to obtain spectra with a spectral resolution of R  = 7000 in the red (540–700 nm) and R  = 3000 in the blue (350–570 nm), over an integral field of 25 × 38 arcsec{sup 2} with 1 × 1 arcsec{sup 2} spatial pixels. The S7 contains both the largest sample of active galaxies and the highest spectral resolution of any comparable integral field survey to date. The emission-line fitting products include line fluxes, velocities, and velocity dispersions across the WiFeS field of view, and an artificial neural network has been used to determine the optimal number of Gaussian kinematic components for emission-lines in each spaxel. Broad Balmer lines are subtracted from the spectra of nuclear spatial pixels in Seyfert 1 galaxies before fitting the narrow lines. We bin nuclear spectra and measure reddening-corrected nuclear fluxes of strong narrow lines for each galaxy. The nuclear spectra are classified on optical diagnostic diagrams, where the strength of the coronal line [Fe vii] λ 6087 is shown to be correlated with [O iii]/H β . Maps revealing gas excitation and kinematics are included for the entire sample, and we provide notes on the newly observed objects.

  2. On-line internal corrosion monitoring and data management for remote pipelines: a technology update

    Energy Technology Data Exchange (ETDEWEB)

    Wold, Kjell; Stoen, Roar; Jenssen, Hallgeir [Roxar Flow Measurement AS, Stavanger (Norway); Carvalho, Anna Maria [Roxar do Brasil Ltda., Rio de Janeiro, RJ (Brazil)

    2009-07-01

    Internal corrosion monitoring of remote pipelines can be costly and demanding on resources. Online and non-intrusive monitoring directly on the pipe wall can improve the quality of measurements, make installation more convenient and allow more efficient communication of data. The purpose of this paper is to describe a non-intrusive technology, and show examples on field installations of the system. Furthermore, the non-intrusive technology data can be stored, interpreted and combined with conventional (intrusive) system information, in order to get a full picture of internal corrosion profile, corrosion rate and trends regarding the pipeline being monitored. (author)

  3. PROBING THE PHYSICS OF NARROW LINE REGIONS IN ACTIVE GALAXIES. II. THE SIDING SPRING SOUTHERN SEYFERT SPECTROSCOPIC SNAPSHOT SURVEY (S7)

    Energy Technology Data Exchange (ETDEWEB)

    Dopita, Michael A.; Davies, Rebecca; Kewley, Lisa; Hampton, Elise; Sutherland, Ralph [RSAA, Australian National University, Cotter Road, Weston Creek, ACT 2611 (Australia); Shastri, Prajval; Kharb, Preeti; Jose, Jessy; Bhatt, Harish; Ramya, S. [Indian Institute of Astrophysics, Koramangala 2 B Block, Bangalore 560034 (India); Scharwächter, Julia [LERMA, Observatoire de Paris, CNRS, UMR 8112, 61 Avenue de l’Observatoire, F-75014 Paris (France); Jin, Chichuan [Qian Xuesen Laboratory for Space Technology, Beijing (China); Banfield, Julie [CSIRO Astronomy and Space Science, P.O. Box 76, Epping NSW, 1710 Australia (Australia); Zaw, Ingyin [New York University (Abu Dhabi), 70 Washington Square South, New York, NY 10012 (United States); Juneau, Stéphanie [CEA-Saclay, DSM/IRFU/SAp, F-91191 Gif-sur-Yvette (France); James, Bethan [Institute of Astronomy, Cambridge University, Madingley Road, Cambridge CB3 0HA (United Kingdom); Srivastava, Shweta, E-mail: Michael.Dopita@anu.edu.au [Astronomy and Astrophysics Division, Physical Research Laboratory, Ahmedabad 380009 (India)

    2015-03-15

    Here we describe the Siding Spring Southern Seyfert Spectroscopic Snapshot Survey (S7) and present results on 64 galaxies drawn from the first data release. The S7 uses the Wide Field Spectrograph mounted on the ANU 2.3 m telescope located at the Siding Spring Observatory to deliver an integral field of 38 × 25 arcsec at a spectral resolution of R = 7000 in the red (530–710 nm), and R = 3000 in the blue (340–560 nm). From these data cubes we have extracted the narrow-line region spectra from a 4 arcsec aperture centered on the nucleus. We also determine the Hβ and [O iii] λ5007 fluxes in the narrow lines, the nuclear reddening, the reddening-corrected relative intensities of the observed emission lines, and the Hβ and [O iii] λ5007 luminosities determined from spectra for which the stellar continuum has been removed. We present a set of images of the galaxies in [O iii] λ5007, [N ii] λ6584, and Hα, which serve to delineate the spatial extent of the extended narrow-line region and also to reveal the structure and morphology of the surrounding H ii regions. Finally, we provide a preliminary discussion of those Seyfert 1 and Seyfert 2 galaxies that display coronal emission lines in order to explore the origin of these lines.

  4. A Design of First-Order Delay-Line DPLL in 1.2μm CMOS Technology

    OpenAIRE

    Seki, Ikuo; Nakashi, Kenichi; Ushida, Mitsuhiko; Taniguchi, Kenji

    1996-01-01

    This paper describes a CMOS 1st-order delay-line DPLL in l.2μm technology for clock regeneration. We have employed a parallel-architecture PC (Phase Comparator) to improve the speed and a DCO (Digitally Controlled Oscillator) without timing hazard. And we have also laid it out in 1.2μm CMOS, and simulated its performance by SPICE as well as logic simulation. Results show that the DPLL operates up to 60MHz, and that lock-in ranges are +5/-5% for regular" 10" input and +5/-5% for 2^13-1 PRBS (P...

  5. Gender Equity On-line: Messages Portrayed with and about the New Technologies.

    Science.gov (United States)

    Knupfer, Nancy Nelson; And Others

    This paper examines gender messages within advertisements and informational, technology-focused materials that are targeted toward a general population of consumers. The pattern of gender bias in visual messages and stereotyping which prevails in advertising appears to be carrying the same messages from print to television and into the newest…

  6. Blurring the Lines: Leveraging Internet Technology for Successful Blending of Secondary/Post-Secondary Technical Education

    Science.gov (United States)

    Ryan, Kenneth; Kopischke, Kevin

    2008-01-01

    The Remote Automation Management Platform (RAMP) is a real-time, interactive teaching tool which leverages common off-the-shelf internet technologies to provide high school learners extraordinary access to advanced technical education opportunities. This outreach paradigm is applicable to a broad range of advanced technical skills from automation…

  7. Are Technology Interruptions Impacting Your Bottom Line? An Innovative Proposal for Change.

    Science.gov (United States)

    Ledbetter, Tamera; Shultz, Sarah; Beckham, Roxanne

    2017-10-01

    Nursing interruptions are a costly and dangerous variable in acute care hospitals. Malfunctioning technology equipment interrupts nursing care and prevents full utilization of computer safety systems to prevent patient care errors. This paper identifies an innovative approach to nursing interruptions related to computer and computer cart malfunctions. The impact on human resources is defined and outcome measures were proposed. A multifaceted proposal, based on a literature review, aimed at reducing nursing interruptions is presented. This proposal is expected to increase patient safety, as well as patient and nurse satisfaction. Acute care hospitals utilizing electronic medical records and bar-coded medication administration technology. Nurses, information technology staff, nursing informatics staff, and all leadership teams affected by technology problems and their proposed solutions. Literature from multiple fields was reviewed to evaluate research related to computer/computer cart failures, and the approaches used to resolve these issues. Outcome measured strategic goals related to patient safety, and nurse and patient satisfaction. Specific help desk metrics will demonstrate the effect of interventions. This paper addresses a gap in the literature and proposes practical and innovative solutions. A comprehensive computer and computer cart repair program is essential for patient safety, financial stewardship, and utilization of resources. © 2015 Wiley Periodicals, Inc.

  8. The individualization of the employment relations on-line: the benefits and limitations of information technologies

    NARCIS (Netherlands)

    Bondarouk, Tatiana; van Riemsdijk, Maarten

    2004-01-01

    One of the latest tendencies in the organisational change theory and practice is implementing e- ideas: e-business, e-commerce, e-library, e-learning, and since not long ago – e-HRM. Software engineers design information technologies (IT) to support HR processes, marketing specialists insist that

  9. Literature Review: Theory and Application of In-Line Inspection Technologies for Oil and Gas Pipeline Girth Weld Defection

    Science.gov (United States)

    Feng, Qingshan; Li, Rui; Nie, Baohua; Liu, Shucong; Zhao, Lianyu; Zhang, Hong

    2016-01-01

    Girth weld cracking is one of the main failure modes in oil and gas pipelines; girth weld cracking inspection has great economic and social significance for the intrinsic safety of pipelines. This paper introduces the typical girth weld defects of oil and gas pipelines and the common nondestructive testing methods, and systematically generalizes the progress in the studies on technical principles, signal analysis, defect sizing method and inspection reliability, etc., of magnetic flux leakage (MFL) inspection, liquid ultrasonic inspection, electromagnetic acoustic transducer (EMAT) inspection and remote field eddy current (RFDC) inspection for oil and gas pipeline girth weld defects. Additionally, it introduces the new technologies for composite ultrasonic, laser ultrasonic, and magnetostriction inspection, and provides reference for development and application of oil and gas pipeline girth weld defect in-line inspection technology. PMID:28036016

  10. Test of the Capability of Laser Line Scan Technology to Support Benthic Habitat Mapping in Coral Reef Ecosystems, Maui Island, November 2006

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The utility of Laser Line Scan (LLS) Technology for optical validation of benthic habitat map data from coral reef ecosystems was tested with a deployment of a...

  11. On-line monitoring of milk electrical conductivity by fuzzy logic technology to characterise health status in dairy goats

    Directory of Open Access Journals (Sweden)

    Mauro Zaninelli

    2014-04-01

    Full Text Available Intramammary infection affects the quality and quantity of dairy goat milk. Health status (HS and milk quality can be monitored by electrical conductivity (EC. The aim of the study was to determine the detection potential of EC when measured on-line on a daily basis and compared with readings from previous milkings. Milk yields (MYs were investigated with the same approach. To evaluate these relative traits, a multivariate model based on fuzzy logic technology – which provided interesting results in cows – was used. Two foremilk samples from 8 healthy Saanen goats were measured daily over the course of six months. Bacteriological tests and somatic cells counts were used to define the HS. On-line EC measurements for each gland and MYs were also considered. Predicted deviations of EC and MY were calculated using a moving-average model and entered in the fuzzy logic model. The reported accuracy has a sensitivity of 81% and a specificity of 69%. Conclusions show that fuzzy logic is an interesting approach for dairy goats, since it offered better accuracy than other methods previously published. Nevertheless, specificity was lower than in dairy cows, probably due to the lack of a significant decrease of MY in diseased glands. Still, results show that the detection of the HS characteristics with EC is improved, when measured on-line, daily and compared with the readings from previous milkings.

  12. AKORT-1 on-line system for technological control of the mixed fuel distribution in fuel elements

    International Nuclear Information System (INIS)

    Baklanov, V.S.; Besednov, G.Yu.; Gadzhiev, G.I.

    1982-01-01

    An on-line system for technological control of experimental fuel elements with vibrocompacted UO 2 -PuO 2 fuel rods fabricated for the BOR-60 reactor is described. Equipment and performance specific features of the system mechanical part and electronic circuits are considered. The results of the system performance testing are given. The fuel element quality sorting is made on the base of the analysis of Pu and fuel density distributions in the rod length. Gamma-absorption method for density measuring and the method of Pu content determination by its own gamma radiation are used in the system simultaneously. The system has the following main characteristics: tested fuel element diameter is 6 mm; the range of fuel rod mean densities is 7-10 g/cm 3 ; Pu content in the fuel is more than 20%; gamma detectors are the NaI(Tl) detectors with dimensions 40x40 and 25x25 mm; energy resolution is 137 Cs gamma line. Electronic circuits of the system operating on-line with the D3-28 microcomputer are made using the VECTOR standard. The system testing has shown that the error in the fuel density determination is less than 1%, that for Pu content measuring is 4%, the system capacity is 6 fuel elements per hour

  13. Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9.

    Science.gov (United States)

    Ren, Xingjie; Sun, Jin; Housden, Benjamin E; Hu, Yanhui; Roesel, Charles; Lin, Shuailiang; Liu, Lu-Ping; Yang, Zhihao; Mao, Decai; Sun, Lingzhu; Wu, Qujie; Ji, Jun-Yuan; Xi, Jianzhong; Mohr, Stephanie E; Xu, Jiang; Perrimon, Norbert; Ni, Jian-Quan

    2013-11-19

    The ability to engineer genomes in a specific, systematic, and cost-effective way is critical for functional genomic studies. Recent advances using the CRISPR-associated single-guide RNA system (Cas9/sgRNA) illustrate the potential of this simple system for genome engineering in a number of organisms. Here we report an effective and inexpensive method for genome DNA editing in Drosophila melanogaster whereby plasmid DNAs encoding short sgRNAs under the control of the U6b promoter are injected into transgenic flies in which Cas9 is specifically expressed in the germ line via the nanos promoter. We evaluate the off-targets associated with the method and establish a Web-based resource, along with a searchable, genome-wide database of predicted sgRNAs appropriate for genome engineering in flies. Finally, we discuss the advantages of our method in comparison with other recently published approaches.

  14. Molecular gas in the Herschel-selected strongly lensed submillimeter galaxies at z 2-4 as probed by multi-J CO lines

    Science.gov (United States)

    Yang, C.; Omont, A.; Beelen, A.; Gao, Y.; van der Werf, P.; Gavazzi, R.; Zhang, Z.-Y.; Ivison, R.; Lehnert, M.; Liu, D.; Oteo, I.; González-Alfonso, E.; Dannerbauer, H.; Cox, P.; Krips, M.; Neri, R.; Riechers, D.; Baker, A. J.; Michałowski, M. J.; Cooray, A.; Smail, I.

    2017-12-01

    We present the IRAM-30 m observations of multiple-J CO (Jup mostly from 3 up to 8) and [C I](3P2 → 3P1) ([C I](2-1) hereafter) line emission in a sample of redshift 2-4 submillimeter galaxies (SMGs). These SMGs are selected among the brightest-lensed galaxies discovered in the Herschel-Astrophysical Terahertz Large Area Survey (H-ATLAS). Forty-seven CO lines and 7 [C I](2-1) lines have been detected in 15 lensed SMGs. A non-negligible effect of differential lensing is found for the CO emission lines, which could have caused significant underestimations of the linewidths, and hence of the dynamical masses. The CO spectral line energy distributions (SLEDs), peaking around Jup 5-7, are found to be similar to those of the local starburst-dominated ultra-luminous infrared galaxies and of the previously studied SMGs. After correcting for lensing amplification, we derived the global properties of the bulk of molecular gas in the SMGs using non-LTE radiative transfer modelling, such as the molecular gas density nH2 102.5-104.1 cm-3 and the kinetic temperature Tk 20-750 K. The gas thermal pressure Pth ranging from 105 K cm-3 to 106 K cm-3 is found to be correlated with star formation efficiency. Further decomposing the CO SLEDs into two excitation components, we find a low-excitation component with nH2 102.8-104.6 cm-3 and Tk 20-30 K, which is less correlated with star formation, and a high-excitation one (nH2 102.7-104.2 cm-3, Tk 60-400 K) which is tightly related to the on-going star-forming activity. Additionally, tight linear correlations between the far-infrared and CO line luminosities have been confirmed for the Jup ≥ 5 CO lines of these SMGs, implying that these CO lines are good tracers of star formation. The [C I](2-1) lines follow the tight linear correlation between the luminosities of the [C I](2-1) and the CO(1-0) line found in local starbursts, indicating that [C I] lines could serve as good total molecular gas mass tracers for high-redshift SMGs as well

  15. Incorporating Oracle on-line space management with long-term archival technology

    Science.gov (United States)

    Moran, Steven M.; Zak, Victor J.

    1996-01-01

    The storage requirements of today's organizations are exploding. As computers continue to escalate in processing power, applications grow in complexity and data files grow in size and in number. As a result, organizations are forced to procure more and more megabytes of storage space. This paper focuses on how to expand the storage capacity of a Very Large Database (VLDB) cost-effectively within a Oracle7 data warehouse system by integrating long term archival storage sub-systems with traditional magnetic media. The Oracle architecture described in this paper was based on an actual proof of concept for a customer looking to store archived data on optical disks yet still have access to this data without user intervention. The customer had a requirement to maintain 10 years worth of data on-line. Data less than a year old still had the potential to be updated thus will reside on conventional magnetic disks. Data older than a year will be considered archived and will be placed on optical disks. The ability to archive data to optical disk and still have access to that data provides the system a means to retain large amounts of data that is readily accessible yet significantly reduces the cost of total system storage. Therefore, the cost benefits of archival storage devices can be incorporated into the Oracle storage medium and I/O subsystem without loosing any of the functionality of transaction processing, yet at the same time providing an organization access to all their data.

  16. The use of radionuclide DNA probe technology for epidemiological studies of tegumentary leishmaniasis in Mato Grosso state

    Directory of Open Access Journals (Sweden)

    Antero Silva Ribeiro de Andrade

    2005-10-01

    Full Text Available DNA hybridisation, using probes labelled with 32P, was used to type Leishmania samples isolated from patients living in endemic areas of Mato Grosso State (Brazil, and clinically diagnosed as having tegumentary leishmaniasis. kDNA cloned mini-circle probes specific for the Leishmania mexicana and Leishmania braziliensis complexes were used. The results showed that L. braziliensis is the predominant group infecting human patients in the state. Sixty-eight samples were typed, 64 samples (94.1% belonging to the L. braziliensis complex and only four (5.9% belonging to the L. mexicana complex. Accurate identification of the Leishmania permits better orientation of the medical follow-up, since clinical manifestations may vary depending on the complex to which the parasite belongs. The epidemiological information furnished by the identification of the Leishmania in given endemic area is also essential for the design of appropriate control measuresHibridização, utilizando sondas de DNA marcadas com 32P, foi utilizada para a tipagem de amostras de Leishmania isoladas de pacientes do estado do Mato Grosso (Brasil, diagnosticados clinicamente como portadores de leishmaniose tegumentar. Sondas de minicírculos clonados de kDNA, específicas para os complexos Leishmania mexicana e Leishmania braziliensis, foram utilizadas. Os resultados demonstraram que o complexo L. brasiliensis é o grupo predominante infectando pacientes humanos no estado do Mato Grosso. Foram tipadas 68 amostras: 64 (94,1% foram identificadas como pertencentes ao complexo L. brasiliensis e somente 4 (5,9% como pertencentes ao complexo L. mexicana. A tipagem de Leishmania é importante para um melhor acompanhamento médico, uma vez que as manifestações clínicas podem variar em função do complexo ao qual o parasita pertence. A informação fornecida pela identificação também é essencial para a definição das medidas de controle mais adequadas e compreensão da epidemiologia da

  17. Coherence peak in the spin susceptibility from nesting in spin-triplet superconductors: A probe for line nodes in Sr2RuO4

    International Nuclear Information System (INIS)

    Yakiyama, Mayumi; Hasegawa, Yasumasa

    2003-01-01

    We study the dynamical spin susceptibility χ(q,ω) for spin-triplet superconductivity. We show that a large peak at ω=2Δ appears in Imχ zz (Q,ω), where z is the direction of the d vector for triplet pairing, if Fermi surface has a nested part with the nesting vector Q and the order parameters are +Δ and -Δ in this part of the Fermi surface. If there are line nodes in the nested part of the Fermi surface, a peak appears in either Imχ zz (Q,ω) or Imχ +- (Q,ω), or both, depending on the perpendicular component of the nesting vector. The comparison with inelastic neutron-scattering experiments can determine the position of the line nodes in triplet superconductor Sr 2 RuO 4

  18. The emission-line regions in the nucleus of NGC 1313 probed with GMOS-IFU: a supergiant/hypergiant candidate and a kinematically cold nucleus

    Science.gov (United States)

    Menezes, R. B.; Steiner, J. E.

    2017-04-01

    NGC 1313 is a bulgeless nearby galaxy, classified as SB(s)d. Its proximity allows high spatial resolution observations. We performed the first detailed analysis of the emission-line properties in the nuclear region of NGC 1313, using an optical data cube obtained with the Gemini Multi-object Spectrograph. We detected four main emitting areas, three of them (regions 1, 2 and 3) having spectra typical of H II regions. Region 1 is located very close to the stellar nucleus and shows broad spectral features characteristic of Wolf-Rayet stars. Our analysis revealed the presence of one or two WC4-5 stars in this region, which is compatible with results obtained by previous studies. Region 4 shows spectral features (as a strong Hα emission line, with a broad component) typical of a massive emission-line star, such as a luminous blue variable, a B[e] supergiant or a B hypergiant. The radial velocity map of the ionized gas shows a pattern consistent with rotation. A significant drop in the values of the gas velocity dispersion was detected very close to region 1, which suggests that the young stars there were formed from this cold gas, possibly keeping low values of velocity dispersion. Therefore, although detailed measurements of the stellar kinematics were not possible (due to the weak stellar absorption spectrum of this galaxy), we predict that NGC 1313 may also show a drop in the values of the stellar velocity dispersion in its nuclear region.

  19. Probe Storage

    NARCIS (Netherlands)

    Gemelli, Marcellino; Abelmann, Leon; Engelen, Johannes Bernardus Charles; Khatib, M.G.; Koelmans, W.W.; Zaboronski, Olog; Campardo, Giovanni; Tiziani, Federico; Laculo, Massimo

    2011-01-01

    This chapter gives an overview of probe-based data storage research over the last three decades, encompassing all aspects of a probe recording system. Following the division found in all mechanically addressed storage systems, the different subsystems (media, read/write heads, positioning, data

  20. Cultural probes

    DEFF Research Database (Denmark)

    Madsen, Jacob Østergaard

    The aim of this study was thus to explore cultural probes (Gaver, Boucher et al. 2004), as a possible methodical approach, supporting knowledge production on situated and contextual aspects of occupation.......The aim of this study was thus to explore cultural probes (Gaver, Boucher et al. 2004), as a possible methodical approach, supporting knowledge production on situated and contextual aspects of occupation....

  1. Master on Photonics and Laser Technologies: on-line teaching experience

    Science.gov (United States)

    Paredes, Ángel; Michinel, Humberto; Salgueiro, José R.; Vázquez-Dorrío, Benito; Yáñez, Armando; Arines, Justo; Flores-Arias, M. Teresa

    2014-07-01

    The Galician Universitary System (SUG) in the framework of the European studies under the Bologna process presents a huge number of Masters courses. In this work we present the teaching framework of the Science Masters on "Photonics and Laser Technologies", coordinated by the University of Vigo (UVigo) and involving the three Universities of Galicia: University of Vigo (UVigo), University of Santiago de Compostela (USC) and University of Coruña (UdC). The aim of this work is to show how teaching at this Masters is carried out using an online platform so that the whole expertise of all the three Universities can be properly exploited and the geographic dispersion of lecturers and students overcame. The used platform permits the students to attend the lessons from their own Universities without wasting time and money on traveling. Besides, each lecturer can teach from his/her own University, allowing the combination of this activity with other professional and scientific duties. Thanks to this tool, the Masters could host students that followed the lessons from other different countries. The platform has been used for lectures, seminar classes, examinations, conferences and coordination activities between teachers and students.

  2. Helping to reduce turbomachinery losses through advanced technology and on-line expertise

    Energy Technology Data Exchange (ETDEWEB)

    Feigel, R.E. [Hartford Steam Boiler Inspection & Insurance Co., Hartford, CT (United States)

    1994-12-31

    It`s clear that turbomachinery poses a set of unique problems for risk managers. The size of the equipment, the role it often takes in production and the severity of a loss all combine to make a risk manager`s job that much more difficult. But while the job may be difficult, it`s not impossible. Through a combination of advanced technology, regular predictive maintenance and some expert advice, today`s risk managers, working with plant operational personnel, are reducing major turbomachinery losses. There are several telltale signs that warn plant personnel of an impending turbomachinery failure. One is vibration. All turbomachinery will vibrate at some level, even when in good working condition. But a change in the vibration level usually indicates a change in the machine`s performance. If plant personnel can detect a change early enough, they may be able to avoid an unscheduled shutdown. Hartford Steam Boiler recently introduced a periodic vibration data collection program called DATALERT{trademark} to help its customers separate problem from non-problem machines. As a result, companies can focus resources on equipment that needs immediate attention. And equipment in good working condition doesn`t tie up resources unnecessarily at the next maintenance turnaround. DATALERT is an integrated machinery vibration data collecting and expert analysis system developed by Hartford Steam Boiler to assist customers in preventing rotating machine downtime or losses. The data collection program is described.

  3. On-line moisture analysis

    CERN Document Server

    Cutmore, N G

    2002-01-01

    Measurement of the moisture content of iron ore has become a key issue for controlling moisture additions for dust suppression. In most cases moisture content is still determined by manual or automatic sampling of the ore stream, followed by conventional laboratory analysis by oven drying. Although this procedure enables the moisture content to be routinely monitored, it is too slow for control purposes. This has generated renewed interest in on-line techniques for the accurate and rapid measurement of moisture in iron ore on conveyors. Microwave transmission techniques have emerged over the past 40 years as the dominant technology for on-line measurement of moisture in bulk materials, including iron ores. Alternative technologies have their limitations. Infra-red analysers are used in a variety of process industries, but rely on the measurement of absorption by moisture in a very thin surface layer. Consequently such probes may be compromised by particle size effects and biased presentation of the bulk mater...

  4. Health information exchange technology on the front lines of healthcare: workflow factors and patterns of use

    Science.gov (United States)

    Johnson, Kevin B; Lorenzi, Nancy M

    2011-01-01

    Objective The goal of this study was to develop an in-depth understanding of how a health information exchange (HIE) fits into clinical workflow at multiple clinical sites. Materials and Methods The ethnographic qualitative study was conducted over a 9-month period in six emergency departments (ED) and eight ambulatory clinics in Memphis, Tennessee, USA. Data were collected using direct observation, informal interviews during observation, and formal semi-structured interviews. The authors observed for over 180 h, during which providers used the exchange 130 times. Results HIE-related workflow was modeled for each ED site and ambulatory clinic group and substantial site-to-site workflow differences were identified. Common patterns in HIE-related workflow were also identified across all sites, leading to the development of two role-based workflow models: nurse based and physician based. The workflow elements framework was applied to the two role-based patterns. An in-depth description was developed of how providers integrated HIE into existing clinical workflow, including prompts for HIE use. Discussion Workflow differed substantially among sites, but two general role-based HIE usage models were identified. Although providers used HIE to improve continuity of patient care, patient–provider trust played a significant role. Types of information retrieved related to roles, with nurses seeking to retrieve recent hospitalization data and more open-ended usage by nurse practitioners and physicians. User and role-specific customization to accommodate differences in workflow and information needs may increase the adoption and use of HIE. Conclusion Understanding end users' perspectives towards HIE technology is crucial to the long-term success of HIE. By applying qualitative methods, an in-depth understanding of HIE usage was developed. PMID:22003156

  5. At-line process analytical technology (PAT) for more efficient scale up of biopharmaceutical microfiltration unit operations.

    Science.gov (United States)

    Watson, Douglas S; Kerchner, Kristi R; Gant, Sean S; Pedersen, Joseph W; Hamburger, James B; Ortigosa, Allison D; Potgieter, Thomas I

    2016-01-01

    Tangential flow microfiltration (MF) is a cost-effective and robust bioprocess separation technique, but successful full scale implementation is hindered by the empirical, trial-and-error nature of scale-up. We present an integrated approach leveraging at-line process analytical technology (PAT) and mass balance based modeling to de-risk MF scale-up. Chromatography-based PAT was employed to improve the consistency of an MF step that had been a bottleneck in the process used to manufacture a therapeutic protein. A 10-min reverse phase ultra high performance liquid chromatography (RP-UPLC) assay was developed to provide at-line monitoring of protein concentration. The method was successfully validated and method performance was comparable to previously validated methods. The PAT tool revealed areas of divergence from a mass balance-based model, highlighting specific opportunities for process improvement. Adjustment of appropriate process controls led to improved operability and significantly increased yield, providing a successful example of PAT deployment in the downstream purification of a therapeutic protein. The general approach presented here should be broadly applicable to reduce risk during scale-up of filtration processes and should be suitable for feed-forward and feed-back process control. © 2015 American Institute of Chemical Engineers.

  6. Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland.

    LENUS (Irish Health Repository)

    Menton, John F

    2007-01-01

    BACKGROUND: Noroviruses are the most common cause of non-bacterial gastroenteritis. Improved detection methods have seen a large increase in the number of human NoV genotypes in the last ten years. The objective of this study was to develop a fast method to detect, quantify and genotype positive NoV samples from Irish hospitals. RESULTS: A real-time RT-PCR assay and a Reverse Line Blot Hybridisation assay were developed based on the ORF1-ORF2 region. The sensitivity and reactivity of the two assays used was validated using a reference stool panel containing 14 NoV genotypes. The assays were then used to investigate two outbreaks of gastroenteritis in two Irish hospitals. 56 samples were screened for NoV using a real-time RT-PCR assay and 26 samples were found to be positive. Genotyping of these positive samples found that all positives belonged to the GII\\/4 variant of NoV. CONCLUSION: The combination of the Real-time assay and the reverse line blot hybridisation assay provided a fast and accurate method to investigate a NoV associated outbreak. It was concluded that the predominant genotype circulating in these Irish hospitals was GII\\/4 which has been associated with the majority of NoV outbreaks worldwide. The assays developed in this study are useful tools for investigating NoV infection.

  7. Probing the Solar System

    Science.gov (United States)

    Wilkinson, John

    2013-01-01

    Humans have always had the vision to one day live on other planets. This vision existed even before the first person was put into orbit. Since the early space missions of putting humans into orbit around Earth, many advances have been made in space technology. We have now sent many space probes deep into the Solar system to explore the planets and…

  8. Mobile probes

    DEFF Research Database (Denmark)

    Ørngreen, Rikke; Jørgensen, Anna Neustrup; Noesgaard, Signe Schack

    2016-01-01

    A project investigating the effectiveness of a collection of online resources for teachers' professional development used mobile probes as a data collection method. Teachers received questions and tasks on their mobile in a dialogic manner while in their everyday context as opposed...... to in an interview. This method provided valuable insight into the contextual use, i.e. how did the online resource transfer to the work practice. However, the research team also found that mobile probes may provide the scaffolding necessary for individual and peer learning at a very local (intra-school) community...... level. This paper is an initial investigation of how the mobile probes process proved to engage teachers in their efforts to improve teaching. It also highlights some of the barriers emerging when applying mobile probes as a scaffold for learning....

  9. Optical probe

    International Nuclear Information System (INIS)

    Denis, J.; Decaudin, J.M.

    1984-01-01

    The probe includes optical means of refractive index n, refracting an incident light beam from a medium with a refractive index n1>n and reflecting an incident light beam from a medium with a refractive index n2 [fr

  10. Counting probe

    International Nuclear Information System (INIS)

    Matsumoto, Haruya; Kaya, Nobuyuki; Yuasa, Kazuhiro; Hayashi, Tomoaki

    1976-01-01

    Electron counting method has been devised and experimented for the purpose of measuring electron temperature and density, the most fundamental quantities to represent plasma conditions. Electron counting is a method to count the electrons in plasma directly by equipping a probe with the secondary electron multiplier. It has three advantages of adjustable sensitivity, high sensitivity of the secondary electron multiplier, and directional property. Sensitivity adjustment is performed by changing the size of collecting hole (pin hole) on the incident front of the multiplier. The probe is usable as a direct reading thermometer of electron temperature because it requires to collect very small amount of electrons, thus it doesn't disturb the surrounding plasma, and the narrow sweep width of the probe voltage is enough. Therefore it can measure anisotropy more sensitively than a Langmuir probe, and it can be used for very low density plasma. Though many problems remain on anisotropy, computer simulation has been carried out. Also it is planned to provide a Helmholtz coil in the vacuum chamber to eliminate the effect of earth magnetic field. In practical experiments, the measurement with a Langmuir probe and an emission probe mounted to the movable structure, the comparison with the results obtained in reverse magnetic field by using a Helmholtz coil, and the measurement of ionic sound wave are scheduled. (Wakatsuki, Y.)

  11. Fed-batch bioreactor performance and cell line stability evaluation of the artificial chromosome expression technology expressing an IgG1 in Chinese hamster ovary cells.

    Science.gov (United States)

    Combs, Rodney G; Yu, Erwin; Roe, Susanna; Piatchek, Michele Bailey; Jones, Heather L; Mott, John; Kennard, Malcolm L; Goosney, Danika L; Monteith, Diane

    2011-01-01

    The artificial chromosome expression (ACE) technology system uses an engineered artificial chromosome containing multiple site-specific recombination acceptor sites for the rapid and efficient construction of stable cell lines. The construction of Chinese hamster ovary(CHO) cell lines expressing an IgG1 monoclonal antibody (MAb) using the ACE system has been previously described (Kennard et al., Biotechnol Bioeng. 2009;104:540-553). To further demonstrate the manufacturing feasibility of the ACE system, four CHO cell lines expressing the human IgG1 MAb 4A1 were evaluated in batch and fed-batch shake flasks and in a 2-L fed-batch bioreactor. The batch shake flasks achieved titers between 0.7 and 1.1 g/L, whereas the fed-batch shake flask process improved titers to 2.5–3.0 g/L. The lead 4A1 ACE cell line achieved titers of 4.0 g/L with an average specific productivity of 40 pg/(cell day) when cultured in a non optimized 2-L fed-batch bioreactor using a completely chemically defined process. Generational stability characterization of the lead 4A1-expressing cell line demonstrated that the cell line was stable for up to 75 days in culture. Product quality attributes of the 4A1 MAb produced by the ACE system during the stability evaluation period were unchanged and also comparable to existing expression technologies such as the CHO-dhfr system. The results of this evaluation demonstrate that a clonal, stable MAb-expressing CHO cell line can be produced using ACE technology that performs competitively using a chemically defined fed-batch bioreactor process with comparable product quality attributes to cell lines generated by existing technologies.

  12. Research on on-line monitoring technology for steel ball's forming process based on load signal analysis method

    Science.gov (United States)

    Li, Ying-jun; Ai, Chang-sheng; Men, Xiu-hua; Zhang, Cheng-liang; Zhang, Qi

    2013-04-01

    This paper presents a novel on-line monitoring technology to obtain forming quality in steel ball's forming process based on load signal analysis method, in order to reveal the bottom die's load characteristic in initial cold heading forging process of steel balls. A mechanical model of the cold header producing process is established and analyzed by using finite element method. The maximum cold heading force is calculated. The results prove that the monitoring on the cold heading process with upsetting force is reasonable and feasible. The forming defects are inflected on the three feature points of the bottom die signals, which are the initial point, infection point, and peak point. A novel PVDF piezoelectric force sensor which is simple on construction and convenient on installation is designed. The sensitivity of the PVDF force sensor is calculated. The characteristics of PVDF force sensor are analyzed by FEM. The PVDF piezoelectric force sensor is fabricated to acquire the actual load signals in the cold heading process, and calibrated by a special device. The measuring system of on-line monitoring is built. The characteristics of the actual signals recognized by learning and identification algorithm are in consistence with simulation results. Identification of actual signals shows that the timing difference values of all feature points for qualified products are not exceed ±6 ms, and amplitude difference values are less than ±3%. The calibration and application experiments show that PVDF force sensor has good static and dynamic performances, and is competent at dynamic measuring on upsetting force. It greatly improves automatic level and machining precision. Equipment capacity factor with damages identification method depends on grade of steel has been improved to 90%.

  13. In-line Fourier-transform infrared spectroscopy as a versatile process analytical technology for preparative protein chromatography.

    Science.gov (United States)

    Großhans, Steffen; Rüdt, Matthias; Sanden, Adrian; Brestrich, Nina; Morgenstern, Josefine; Heissler, Stefan; Hubbuch, Jürgen

    2018-04-27

    Fourier-transform infrared spectroscopy (FTIR) is a well-established spectroscopic method in the analysis of small molecules and protein secondary structure. However, FTIR is not commonly applied for in-line monitoring of protein chromatography. Here, the potential of in-line FTIR as a process analytical technology (PAT) in downstream processing was investigated in three case studies addressing the limits of currently applied spectroscopic PAT methods. A first case study exploited the secondary structural differences of monoclonal antibodies (mAbs) and lysozyme to selectively quantify the two proteins with partial least squares regression (PLS) giving root mean square errors of cross validation (RMSECV) of 2.42 g/l and 1.67 g/l, respectively. The corresponding Q 2 values are 0.92 and, respectively, 0.99, indicating robust models in the calibration range. Second, a process separating lysozyme and PEGylated lysozyme species was monitored giving an estimate of the PEGylation degree of currently eluting species with RMSECV of 2.35 g/l for lysozyme and 1.24 g/l for PEG with Q 2 of 0.96 and 0.94, respectively. Finally, Triton X-100 was added to a feed of lysozyme as a typical process-related impurity. It was shown that the species could be selectively quantified from the FTIR 3D field without PLS calibration. In summary, the proposed PAT tool has the potential to be used as a versatile option for monitoring protein chromatography. It may help to achieve a more complete implementation of the PAT initiative by mitigating limitations of currently used techniques. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

  14. Estimating the Theoretical Value for LAN Network Throughput Based Power Line Communications Technology Under the Homeplug 1.0 Standard

    Directory of Open Access Journals (Sweden)

    Martha Fabiola Contreras Higuera

    2013-06-01

    Full Text Available Power Line Communications (PLC refers to a group of technologies that allow to establish communication processes under the use of the grid as a physical means of transmission. The use of the grid as a physical means of transmission of information is not a new idea. Until a few years ago, the use of PLC had been limited to the implementation of solutions of control, automation and monitoring of sensors; which did not require a high bandwidth for its operation.During the late 1990s due to the new technological developments and the need to implement new alternatives for transfer of information, it was possible to reach speeds on the order of the Mbps, establishing the possibility of making use of the electricity network as a network of access. The current state of technology PLC allows to reach speeds of up to 200Mbps, which has enabled the transformation of the grid in a true network of band wide, capable of supporting data, voice and video provided by a telecommunications operator. The use of PLC-based network adapters allow easily design LANs and broadband communications through the electrical network, making any outlet in a point of connection for the user, without the need for wiring additional to existing ones.  The electrical network is a structure which so far has been exclusively used for the transport of electrical energy. However, it is possible to make use of this network in processes of communication and transmission of information such as: voice, data and video; Bearing in mind that grid had not been designed for this purpose. The performance is without doubt one of the aspects of greatest interest in the global analysis in networks LAN, due to the effect it produces on the end user. Basically, the most common parameters for evaluating the performance of a network are: Throughput, use of the canal and various measures of retardation. In this article is presented a simple analysis of the HomePlug 1.0 standard applied to the

  15. Technology

    Directory of Open Access Journals (Sweden)

    Xu Jing

    2016-01-01

    Full Text Available The traditional answer card reading method using OMR (Optical Mark Reader, most commonly, OMR special card special use, less versatile, high cost, aiming at the existing problems proposed a method based on pattern recognition of the answer card identification method. Using the method based on Line Segment Detector to detect the tilt of the image, the existence of tilt image rotation correction, and eventually achieve positioning and detection of answers to the answer sheet .Pattern recognition technology for automatic reading, high accuracy, detect faster

  16. DNA probes

    International Nuclear Information System (INIS)

    Castelino, J.

    1992-01-01

    The creation of DNA probes for detection of specific nucleotide segments differs from ligand detection in that it is a chemical rather than an immunological reaction. Complementary DNA or RNA is used in place of the antibody and is labelled with 32 P. So far, DNA probes have been successfully employed in the diagnosis of inherited disorders, infectious diseases, and for identification of human oncogenes. The latest approach to the diagnosis of communicable and parasitic infections is based on the use of deoxyribonucleic acid (DNA) probes. The genetic information of all cells is encoded by DNA and DNA probe approach to identification of pathogens is unique because the focus of the method is the nucleic acid content of the organism rather than the products that the nucleic acid encodes. Since every properly classified species has some unique nucleotide sequences that distinguish it from every other species, each organism's genetic composition is in essence a finger print that can be used for its identification. In addition to this specificity, DNA probes offer other advantages in that pathogens may be identified directly in clinical specimens

  17. DNA probes

    Energy Technology Data Exchange (ETDEWEB)

    Castelino, J

    1993-12-31

    The creation of DNA probes for detection of specific nucleotide segments differs from ligand detection in that it is a chemical rather than an immunological reaction. Complementary DNA or RNA is used in place of the antibody and is labelled with {sup 32}P. So far, DNA probes have been successfully employed in the diagnosis of inherited disorders, infectious diseases, and for identification of human oncogenes. The latest approach to the diagnosis of communicable and parasitic infections is based on the use of deoxyribonucleic acid (DNA) probes. The genetic information of all cells is encoded by DNA and DNA probe approach to identification of pathogens is unique because the focus of the method is the nucleic acid content of the organism rather than the products that the nucleic acid encodes. Since every properly classified species has some unique nucleotide sequences that distinguish it from every other species, each organism`s genetic composition is in essence a finger print that can be used for its identification. In addition to this specificity, DNA probes offer other advantages in that pathogens may be identified directly in clinical specimens 10 figs, 2 tabs

  18. Near-Infrared Spectroscopy as an Analytical Process Technology for the On-Line Quantification of Water Precipitation Processes during Danhong Injection

    Directory of Open Access Journals (Sweden)

    Xuesong Liu

    2015-01-01

    Full Text Available This paper used near-infrared (NIR spectroscopy for the on-line quantitative monitoring of water precipitation during Danhong injection. For these NIR measurements, two fiber optic probes designed to transmit NIR radiation through a 2 mm flow cell were used to collect spectra in real-time. Partial least squares regression (PLSR was developed as the preferred chemometrics quantitative analysis of the critical intermediate qualities: the danshensu (DSS, (R-3, 4-dihydroxyphenyllactic acid, protocatechuic aldehyde (PA, rosmarinic acid (RA, and salvianolic acid B (SAB concentrations. Optimized PLSR models were successfully built and used for on-line detecting of the concentrations of DSS, PA, RA, and SAB of water precipitation during Danhong injection. Besides, the information of DSS, PA, RA, and SAB concentrations would be instantly fed back to site technical personnel for control and adjustment timely. The verification experiments determined that the predicted values agreed with the actual homologic value.

  19. Near-Infrared Spectroscopy as an Analytical Process Technology for the On-Line Quantification of Water Precipitation Processes during Danhong Injection.

    Science.gov (United States)

    Liu, Xuesong; Wu, Chunyan; Geng, Shu; Jin, Ye; Luan, Lianjun; Chen, Yong; Wu, Yongjiang

    2015-01-01

    This paper used near-infrared (NIR) spectroscopy for the on-line quantitative monitoring of water precipitation during Danhong injection. For these NIR measurements, two fiber optic probes designed to transmit NIR radiation through a 2 mm flow cell were used to collect spectra in real-time. Partial least squares regression (PLSR) was developed as the preferred chemometrics quantitative analysis of the critical intermediate qualities: the danshensu (DSS, (R)-3, 4-dihydroxyphenyllactic acid), protocatechuic aldehyde (PA), rosmarinic acid (RA), and salvianolic acid B (SAB) concentrations. Optimized PLSR models were successfully built and used for on-line detecting of the concentrations of DSS, PA, RA, and SAB of water precipitation during Danhong injection. Besides, the information of DSS, PA, RA, and SAB concentrations would be instantly fed back to site technical personnel for control and adjustment timely. The verification experiments determined that the predicted values agreed with the actual homologic value.

  20. Study of data transmission over drive motor power cables of a remotely operated robot using power line communication technology

    International Nuclear Information System (INIS)

    Balaji, Kanchi; Singh, Ashutosh Pratap; Rakesh, V.; Rajagopalan, C.; Murugan, S.

    2016-01-01

    In nuclear power plants (NPPs), robotic devices are widely used for carrying out in-service inspection (lSI) and repair tasks at elevated temperatures and radiation environments. These devices comprises servo motors that are powered and controlled by the motion control unit/electric drives. The control and inspection data are to be transmitted back from the inspection device to the control unit using separate industrial graded data transmission cables compatible for high temperature applications. This increases complexity of robot trailing cables and cost. Hence, it is proposed to use power cables that are used for powering the motor for sending information signals using power line communication (PLC) technology. The domestically used PLC modems adds noise to the information signal when coupled on to the motor feed cables during IGBT switching. Therefore, passive low pass filters are used at the motor drive end to reduce the impulsive noise. The aim of this study is to compare noise characteristics for different type of filter structures over pulse width modulation (PWM) network. We will highlight the choice of cut off frequency of filter based on frequency of information signal

  1. Conductivity Probe

    Science.gov (United States)

    2008-01-01

    The Thermal and Electrical Conductivity Probe (TECP) for NASA's Phoenix Mars Lander took measurements in Martian soil and in the air. The needles on the end of the instrument were inserted into the Martian soil, allowing TECP to measure the propagation of both thermal and electrical energy. TECP also measured the humidity in the surrounding air. The needles on the probe are 15 millimeters (0.6 inch) long. The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development is by Lockheed Martin Space Systems, Denver.

  2. Comparative evaluation of GenoType MTBDRplus line probe assay with solid culture method in early diagnosis of multidrug resistant tuberculosis (MDR-TB at a tertiary care centre in India.

    Directory of Open Access Journals (Sweden)

    Raj N Yadav

    Full Text Available The objectives of the study were to compare the performance of line probe assay (GenoType MTBDRplus with solid culture method for an early diagnosis of multidrug resistant tuberculosis (MDR-TB, and to study the mutation patterns associated with rpoB, katG and inhA genes at a tertiary care centre in north India.In this cross-sectional study, 269 previously treated sputum-smear acid-fast bacilli (AFB positive MDR-TB suspects were enrolled from January to September 2012 at the All India Institute of Medical Sciences hospital, New Delhi. Line probe assay (LPA was performed directly on the sputum specimens and the results were compared with that of conventional drug susceptibility testing (DST on solid media [Lowenstein Jensen (LJ method].DST results by LPA and LJ methods were compared in 242 MDR-TB suspects. The LPA detected rifampicin (RIF resistance in 70 of 71 cases, isoniazid (INH resistance in 86 of 93 cases, and MDR-TB in 66 of 68 cases as compared to the conventional method. Overall (rifampicin, isoniazid and MDR-TB concordance of the LPA with the conventional DST was 96%. Sensitivity and specificity were 98% and 99% respectively for detection of RIF resistance; 92% and 99% respectively for detection of INH resistance; 97% and 100% respectively for detection of MDR-TB. Frequencies of katG gene, inhA gene and combined katG and inhA gene mutations conferring all INH resistance were 72/87 (83%, 10/87 (11% and 5/87 (6% respectively. The turnaround time of the LPA test was 48 hours.The LPA test provides an early diagnosis of monoresistance to isoniazid and rifampicin and is highly sensitive and specific for an early diagnosis of MDR-TB. Based on these findings, it is concluded that the LPA test can be useful in early diagnosis of drug resistant TB in high TB burden countries.

  3. Probe specificity

    International Nuclear Information System (INIS)

    Laget, J.M.

    1986-11-01

    Specificity and complementarity of hadron and electron probes must be systematically developed to answer three questions currently asked in intermediate energy nuclear physics: what is nucleus structure at short distances, what is nature of short range correlations, what is three body force nature [fr

  4. Probing the Terrain

    DEFF Research Database (Denmark)

    Johannessen, Runa

    2016-01-01

    Whether manifest in built structures or invisible infrastructures, architectures of control in the occupied Palestinian West Bank is structurally defined by endemic uncertainty. Shifting lines and frontiers are recorded on the terrain, creating elastic zones of uncertainty necessitating navigatio...... to the territory through its lines and laws, and how the very structure of the occupation has changed over the years, I seek to make visible the ways in which architectures of uncertainty compensate for the fleeting terrain that HH is probing.......Whether manifest in built structures or invisible infrastructures, architectures of control in the occupied Palestinian West Bank is structurally defined by endemic uncertainty. Shifting lines and frontiers are recorded on the terrain, creating elastic zones of uncertainty necessitating...

  5. The introgression of RNAi silencing of γ-gliadins into commercial lines of bread wheat changes the mixing and technological properties of the dough.

    Directory of Open Access Journals (Sweden)

    Javier Gil-Humanes

    Full Text Available In the present work the effects on dough quality by the down-regulation of γ-gliadins in different genetic backgrounds of bread wheat were investigated. RNAi-mediated silencing of γ-gliadins was introgressed by conventional crossing into three commercial bread wheat lines (namely 'Gazul', 'Podenco' and 'Arpain', and along with the transgenic line A1152 (cv. Bobwhite compared with their respective wild types. The protein fractions were quantified by RP-HPLC, whereas the technological and mixing properties were assessed by SDSS test and by the Mixograph instrument. Principal component analysis (PCA was carried out for both the wild types and the transgenic lines, showing differences in the factors affecting the technological and mixing properties of the dough as a consequence of the reduction of the γ-gliadins. In transgenic lines, the α- and ω-gliadins, and total gliadins negatively affected the dough strength and tolerance to over-mixing, whereas the L/H ratio showed the opposite effect, positively influencing the dough quality. The increase of the SDSS volume in the transgenic lines of 'Gazul', 'Podenco' and 'Arpain' indicates increased gluten strength and quality respect to the wild types. SDSS volume was found to be positively influenced by the amount of glutenins, which were also increased in the transgenic lines. In addition, a positive effect was observed in the MT, PR1 and RBD in some of the transgenic lines of 'Podenco' and 'Arpain'. In conclusion, the down-regulation of γ-gliadins resulted in stronger doughs and a better tolerance to over-mixing in some transgenic lines. Although the reduction of γ-gliadins seems not to have a direct effect on the mixing and bread-making properties, the compensatory effect on the synthesis of the other prolamins may result in stronger doughs with improved over-mixing resistance.

  6. The introgression of RNAi silencing of γ-gliadins into commercial lines of bread wheat changes the mixing and technological properties of the dough.

    Science.gov (United States)

    Gil-Humanes, Javier; Pistón, Fernando; Giménez, María J; Martín, Antonio; Barro, Francisco

    2012-01-01

    In the present work the effects on dough quality by the down-regulation of γ-gliadins in different genetic backgrounds of bread wheat were investigated. RNAi-mediated silencing of γ-gliadins was introgressed by conventional crossing into three commercial bread wheat lines (namely 'Gazul', 'Podenco' and 'Arpain'), and along with the transgenic line A1152 (cv. Bobwhite) compared with their respective wild types. The protein fractions were quantified by RP-HPLC, whereas the technological and mixing properties were assessed by SDSS test and by the Mixograph instrument. Principal component analysis (PCA) was carried out for both the wild types and the transgenic lines, showing differences in the factors affecting the technological and mixing properties of the dough as a consequence of the reduction of the γ-gliadins. In transgenic lines, the α- and ω-gliadins, and total gliadins negatively affected the dough strength and tolerance to over-mixing, whereas the L/H ratio showed the opposite effect, positively influencing the dough quality. The increase of the SDSS volume in the transgenic lines of 'Gazul', 'Podenco' and 'Arpain' indicates increased gluten strength and quality respect to the wild types. SDSS volume was found to be positively influenced by the amount of glutenins, which were also increased in the transgenic lines. In addition, a positive effect was observed in the MT, PR1 and RBD in some of the transgenic lines of 'Podenco' and 'Arpain'. In conclusion, the down-regulation of γ-gliadins resulted in stronger doughs and a better tolerance to over-mixing in some transgenic lines. Although the reduction of γ-gliadins seems not to have a direct effect on the mixing and bread-making properties, the compensatory effect on the synthesis of the other prolamins may result in stronger doughs with improved over-mixing resistance.

  7. Trigo: avaliação tecnológica de novas linhagens Technological evaluation of new lines of wheat

    Directory of Open Access Journals (Sweden)

    Celina Raquel de Oliveira Camargo

    1987-01-01

    Full Text Available Avaliou-se a qualidade tecnológica das linhagens de trigo IAC-22, IAC-31, IAC-37, IAC-41, IAC-46, IAC-57 e IAC-60, obtidas pelo programa de melhoramento do Instituto Agronômico, tomando como controle uma amostra de trigo norte-americano e amostras dos cultivares Alondra-S-46 e IAC-18, comerciais no Estado de São Paulo. Os maiores teores de proteína foram encontrados no 'IAC-22' e nas linhagens IAC-37 e IAC-41, superiores àqueles das farinhas de trigo importado, Alondra-S-46 e IAC-18. Todas as farinhas de trigo apresentaram viscosidade máxima superior a 1.000 unidades amilográficas, indicando a ausência da enzima alfa-amilase. As farinhas de trigo importado e das linhagens IAC-31, IAC-41 e IAC-57 apresentaram características farinográficas típicas de farinha de força média a forte e IAC-18 e IAC-60, de farinha média a fraca. Os extensigramas mostraram que as linhagens IAC-41, IAC-31, IAC-57 e IAC-46 apresentaram glúten com características viscoelásticas adequadas para a produção de pão. Pelo teste de panificação, concluiu-se que as linhagens IAC-31 e IAC-41 produziram pão de qualidade "muito boa", semelhante à da farinha de trigo importado; IAC-57, IAC-37 e IAC-60, pão de qualidade "boa" e similar à do 'Alondra-S-46'; as linhagens IAC-46 e IAC-22 tiveram o pior comportamento em relação à qualidade de pão, "regular", semelhante à do 'IAC-18'.The new wheat lines IAC-22, IAC-31, IAC-37, IAC-41, IAC-46, IAC-57 and IAC-60, obtained from the Wheat Breeding Program of the Instituto Agronômico of the State of São Paulo, Brazil, were submitted to technological tests to evaluate the flour quality. Samples of wheat from the United States and of the wheat cultivars Alondra-S-46 and IAC-18 were used as controls. The wheat flours were submitted to chemical tests to determine the protein, lipid, ash, fiber and carbohidrate composition and to rheologic tests using a farinograph, an extensigraph and a viscoamilograph. The final

  8. Research, Calculation for Designing the Technological Equipment Line of Cementation with 3,000 Tonnes of Solid Waste/Year Capacity for ZOC Plan

    International Nuclear Information System (INIS)

    Tran Van Hoa; Ngo Van Tuyen; Nguyen Ba Tien; Le Ba Thuan; Phung Quoc Khanh; Mai Duc Lanh; Vuong Huu Anh; Luu Cao Nguyen; Duong Dinh Tho; Phung Vu Phong; Nguyen An Thai; Pham Quang Minh

    2013-01-01

    Technological process and equipment line for cementation with 3,000 tons of solid wastes/year capacity for ZOC plant was studied according to the quantity, the composition and the property of solid wastes from ZOC plant. The influence of several parameters with cementation process such as cement mixing ratio, size, moisture and NaCl content in residue, which affected to mechanical and chemical strength of waste block after cementation was evaluated. The calculation and designing for cementation equipment line were thus conducted and total investment cost of construction and installation as well as operation of this equipment line was also estimated. Radiation safety and environmental protection for waste treatment facility was calculated and the design on radiation safety and environmental protection for equipment line was proposed. Technological process and design document of this production line seemed to be reasonable because it is consistent with properties of wastes as well as it has been commonly used in the world to stabilize solid residues in nuclear industry. The advantages of this propose were simple structure of the devices, locally made, easy operation, locally available and inexpensive materials resulting in low cost of investment and operation. (author)

  9. Mobile Probes in Mobile Learning

    DEFF Research Database (Denmark)

    Ørngreen, Rikke; Blomhøj, Ulla; Duvaa, Uffe

    In this paper experiences from using mobile probes in educational design of a mobile learning application is presented. The probing process stems from the cultural probe method, and was influenced by qualitative interview and inquiry approaches. In the project, the mobile phone was not only acting...... as an agent for acquiring empirical data (as the situation in hitherto mobile probe settings) but was also the technological medium for which data should say something about (mobile learning). Consequently, not only the content of the data but also the ways in which data was delivered and handled, provided...... a valuable dimension for investigating mobile use. The data was collected at the same time as design activities took place and the collective data was analysed based on user experience goals and cognitive processes from interaction design and mobile learning. The mobile probe increased the knowledge base...

  10. On-line moisture analysis

    International Nuclear Information System (INIS)

    Cutmore, N.G.; Mijak, D.G

    2002-01-01

    Measurement of the moisture content of iron ore has become a key issue for controlling moisture additions for dust suppression. In most cases moisture content is still determined by manual or automatic sampling of the ore stream, followed by conventional laboratory analysis by oven drying. Although this procedure enables the moisture content to be routinely monitored, it is too slow for control purposes. This has generated renewed interest in on-line techniques for the accurate and rapid measurement of moisture in iron ore on conveyors. Microwave transmission techniques have emerged over the past 40 years as the dominant technology for on-line measurement of moisture in bulk materials, including iron ores. Alternative technologies have their limitations. Infra-red analysers are used in a variety of process industries, but rely on the measurement of absorption by moisture in a very thin surface layer. Consequently such probes may be compromised by particle size effects and biased presentation of the bulk material. Nuclear-based analysers measure the total hydrogen content in the sample and do not differentiate between free and combined moisture. Such analysers may also be sensitive to material presentation and elemental composition. Very low frequency electromagnetic probes, such as capacitance or conductance probes, operate in the frequency region where the DC conductivity dominates much of the response, which is a function not only of moisture content but also of ionic composition and chemistry. These problems are overcome using microwave transmission techniques, which also have the following advantages, as a true bulk moisture analysis is obtained, because a high percentage of the bulk material is analysed; the moisture estimate is mostly insensitive to any biased presentation of moisture, for example due to stratification of bulk material with different moisture content and because no physical contact is made between the sensor and the bulk material. This is

  11. Advanced development of particle-beam-probe diagnostic systems. Technical progress report, 1 July 1980-30 April 1981

    International Nuclear Information System (INIS)

    Hickok, R.L.; Jennings, W.C.; Woo, J.T.; Connor, K.A.

    1981-05-01

    The heavy ion beam probe system on the RENTOR tokamak has been reinstalled with considerably improved performance. The heavy neutral beam probe system on the ALEX baseball facility has demonstrated the capability of measuring space potential in minimum-B geometry. A large amount of data were obtained from the highly successful TMX beam probe system and are presently being analyzed. Technological improvements were made on both the RENTOR and ALEX diagnostic systems, new ion sources and extraction configurations were investigated, and the superiority of off-line processing techniques for beam probe data has been demonstrated. The development of high energy probing beams for application to major confinement experiments has been initiated and cross-over sweep systems to improve spatial resolution, differential pumping, and reduce energy requirements have been designed

  12. Analysis of dynamic accumulative damage about the lining structure of high speed railway’s tunnel based on ultrasonic testing technology

    Science.gov (United States)

    Wang, Xiang-qiu; Zhang, Huojun; Xie, Wen-xi

    2017-08-01

    Based on the similar material model test of full tunnel, the theory of elastic wave propagation and the testing technology of intelligent ultrasonic wave had been used to research the dynamic accumulative damage characteristics of tunnel’s lining structure under the dynamic loads of high speed train. For the more, the dynamic damage variable of lining structure of high speed railway’s tunnel was obtained. The results shown that the dynamic cumulative damage of lining structure increases nonlinearly with the times of cumulative vibration, the weakest part of dynamic cumulative damage is the arch foot of tunnel. Much more attention should be paid to the design and operation management of high speed railway’s tunnel.

  13. The time domain triple probe method

    International Nuclear Information System (INIS)

    Meier, M.A.; Hallock, G.A.; Tsui, H.Y.W.; Bengtson, R.D.

    1994-01-01

    A new Langmuir probe technique based on the triple probe method is being developed to provide simultaneous measurement of plasma temperature, potential, and density with the temporal and spatial resolution required to accurately characterize plasma turbulence. When the conventional triple probe method is used in an inhomogeneous plasma, local differences in the plasma measured at each probe introduce significant error in the estimation of turbulence parameters. The Time Domain Triple Probe method (TDTP) uses high speed switching of Langmuir probe potential, rather than spatially separated probes, to gather the triple probe information thus avoiding these errors. Analysis indicates that plasma response times and recent electronics technology meet the requirements to implement the TDTP method. Data reduction techniques of TDTP data are to include linear and higher order correlation analysis to estimate fluctuation induced particle and thermal transport, as well as energy relationships between temperature, density, and potential fluctuations

  14. Development of technology and equipment for trapping emissions from a vitrification line, including the manufacture of selected nodes (flow separator, NOx absorber, aerosol filters)

    International Nuclear Information System (INIS)

    Kepak, F.; Kanka, J.; Koutova, S.; Petioky, K.; Seidlova, B.

    1993-03-01

    The technology was designed of a processing line for trapping nitrogen oxides emerging during the treatment of liquid wastes from nuclear power plants. In this design, absorption with a chemical reaction is combined with subsequent trapping of the then formed aerosols of the acids on a sprayed fibrous insert flown through by the gas treated. The degree of absorption is 94% and more for NO 2 , and 43% on average for NO. (J.B). 10 tabs., 3 figs., 11 refs

  15. Species detection using HyBeacon(®) probe technology: Working towards rapid onsite testing in non-human forensic and food authentication applications.

    Science.gov (United States)

    Dawnay, Nick; Hughes, Rebecca; Court, Denise Syndercombe; Duxbury, Nicola

    2016-01-01

    Identifying individual species or determining species' composition in an unknown sample is important for a variety of forensic applications. Food authentication, monitoring illegal trade in endangered species, forensic entomology, sexual assault case work and counter terrorism are just some of the fields that can require the detection of the biological species present. Traditional laboratory based approaches employ a wide variety of tools and technologies and exploit a number of different species specific traits including morphology, molecular differences and immuno-chemical analyses. A large number of these approaches require laboratory based apparatus and results can take a number of days to be returned to investigating authorities. Having a presumptive test for rapid identification could lead to savings in terms of cost and time and allow sample prioritisation if confirmatory testing in a laboratory is required later. This model study describes the development of an assay using a single HyBeacon(®) probe and melt curve analyses allowing rapid screening and authentication of food products labelled as Atlantic cod (Gadus morhua). Exploiting melt curve detection of species specific SNP sites on the COI gene the test allows detection of a target species (Atlantic cod) and closely related species which may be used as substitutes. The assay has been designed for use with the Field Portable ParaDNA system, a molecular detection platform for non-expert users. The entire process from sampling to result takes approximately 75min. Validation studies were performed on both single source genomic DNA, mixed genomic DNA and commercial samples. Data suggests the assay has a lower limit of detection of 31 pg DNA. The specificity of the assay to Atlantic cod was measured by testing highly processed food samples including frozen, defrosted and cooked fish fillets as well as fish fingers, battered fish fillet and fish pie. Ninety-six (92.7%) of all Atlantic cod food products

  16. Mjollnir Rotational Line Scan Diagnostics.

    Science.gov (United States)

    1981-05-19

    using long cavity. M8 Removable Pellicle Beam Splitter for He-Ne Lineup Beam. Removed before HF or DF laser is turned on. 27 A 27 * A r of the chopper...three probe laser lines, however three lines were sequentially measured to verify the diagnostic equipment. Two of the three lines have been monitored

  17. Development of acoustically lined ejector technology for multitube jet noise suppressor nozzles by model and engine tests over a wide range of jet pressure ratios and temperatures

    Science.gov (United States)

    Atvars, J.; Paynter, G. C.; Walker, D. Q.; Wintermeyer, C. F.

    1974-01-01

    An experimental program comprising model nozzle and full-scale engine tests was undertaken to acquire parametric data for acoustically lined ejectors applied to primary jet noise suppression. Ejector lining design technology and acoustical scaling of lined ejector configurations were the major objectives. Ground static tests were run with a J-75 turbojet engine fitted with a 37-tube, area ratio 3.3 suppressor nozzle and two lengths of ejector shroud (L/D = 1 and 2). Seven ejector lining configurations were tested over the engine pressure ratio range of 1.40 to 2.40 with corresponding jet velocities between 305 and 610 M/sec. One-fourth scale model nozzles were tested over a pressure ratio range of 1.40 to 4.0 with jet total temperatures between ambient and 1088 K. Scaling of multielement nozzle ejector configurations was also studied using a single element of the nozzle array with identical ejector lengths and lining materials. Acoustic far field and near field data together with nozzle thrust performance and jet aerodynamic flow profiles are presented.

  18. Using ice melting and ice rolling technologies to remove ice from sub-transmission and transmission lines at Manitoba Hydro

    International Nuclear Information System (INIS)

    Farias, A. R.

    1999-01-01

    Development of an of an Ice Storm Management program by Manitoba Hydro to reduce ice storm damage to its 8 kV feeders to 115 kV transmission lines, is discussed. The program consists of the de-icing of overhead lines, either by ice melting, or ice rolling. Ice melting involves the placement of a three-phase short at a calculated point. The term ice rolling denotes a process of mechanically stripping the ice from conductors. The most recent major ice storm experienced by Manitoba Hydro was in the winter of 1997/1998. During the period from February 6 to February 17, 1998, a total of 83 'ice melt' procedures were performed to melt the ice from 2,628 km of overhead line (7,883 km of conductor), in addition to 'ice rolling'. This paper describes Manitoba Hydro's 25-years' experience with ice melting and it also describes the advantages and disadvantages of both ice melting and ice rolling. Although not a panacea to combat the effects of ice storms, ice melting was found to be the most effective way of removing ice from overhead transmission and sub-transmission lines. Ice rolling was also found to be effective. Other tools that have been found to be useful by various utilities in combating ice storm damage include improved structure and line design, system design that provide more redundancies and emergency sources, and standby generators at critical load points

  19. Image processing for HTS SQUID probe microscope

    International Nuclear Information System (INIS)

    Hayashi, T.; Koetitz, R.; Itozaki, H.; Ishikawa, T.; Kawabe, U.

    2005-01-01

    An HTS SQUID probe microscope has been developed using a high-permeability needle to enable high spatial resolution measurement of samples in air even at room temperature. Image processing techniques have also been developed to improve the magnetic field images obtained from the microscope. Artifacts in the data occur due to electromagnetic interference from electric power lines, line drift and flux trapping. The electromagnetic interference could successfully be removed by eliminating the noise peaks from the power spectrum of fast Fourier transforms of line scans of the image. The drift between lines was removed by interpolating the mean field value of each scan line. Artifacts in line scans occurring due to flux trapping or unexpected noise were removed by the detection of a sharp drift and interpolation using the line data of neighboring lines. Highly detailed magnetic field images were obtained from the HTS SQUID probe microscope by the application of these image processing techniques

  20. Hanford stakeholder participation in evaluating innovative technologies: VOC product line, Passive soil vapor extraction using borehole flux tunable hybrid plasma

    International Nuclear Information System (INIS)

    Peterson, T.; McCabe, G.; Niesen, K.; Serie, P.

    1995-05-01

    A three-phased stakeholder participation program was conducted to support the Volatile Organic Compounds Arid Site Integrated Demonstration (VOC-Arid ID). The US DOE's Office of Technology Development (OTD) sponsored and directed the VOC-Arid ID. Its purpose was to develop and demonstrate new technologies for remediating VOC contamination in soil and ground water. The integrated demonstration, hosted by the Hanford site in Washington State, is being transitioned into the Department of Energy's (DOE) Plume Focus Area. The Plume Focus Area has the same basic objectives as the ID, but is broader in scope and is a team effort with technology developers and technology users. The objective is to demonstrate a promising technology once, and if results warrant deploy it broadly across the DOE complex and in private sector applications

  1. In-line and Real-time Monitoring of Resonant Acoustic Mixing by Near-infrared Spectroscopy Combined with Chemometric Technology for Process Analytical Technology Applications in Pharmaceutical Powder Blending Systems.

    Science.gov (United States)

    Tanaka, Ryoma; Takahashi, Naoyuki; Nakamura, Yasuaki; Hattori, Yusuke; Ashizawa, Kazuhide; Otsuka, Makoto

    2017-01-01

    Resonant acoustic ® mixing (RAM) technology is a system that performs high-speed mixing by vibration through the control of acceleration and frequency. In recent years, real-time process monitoring and prediction has become of increasing interest, and process analytical technology (PAT) systems will be increasingly introduced into actual manufacturing processes. This study examined the application of PAT with the combination of RAM, near-infrared spectroscopy, and chemometric technology as a set of PAT tools for introduction into actual pharmaceutical powder blending processes. Content uniformity was based on a robust partial least squares regression (PLSR) model constructed to manage the RAM configuration parameters and the changing concentration of the components. As a result, real-time monitoring may be possible and could be successfully demonstrated for in-line real-time prediction of active pharmaceutical ingredients and other additives using chemometric technology. This system is expected to be applicable to the RAM method for the risk management of quality.

  2. The features of the use of GIS technologies for monitoring of the situation of main water lines in Azerbaijan

    Directory of Open Access Journals (Sweden)

    M. H. Gojamanov

    2014-11-01

    a comprehensive attract the materials of the aerospace sensing and GIS technologies. In this paper describe the work experience and are the results of monitoring of pipelines of water supply in Azerbaijan.

  3. The solar probe mission

    International Nuclear Information System (INIS)

    Feldman, W.C.; Anderson, J.; Bohlin, J.D.; Burlaga, L.F.; Farquhar, R.; Gloeckler, G.; Goldstein, B.E.; Harvey, J.W.; Holzer, T.E.; Jones, W.V.; Kellogg, P.J.; Krimigis, S.M.; Kundu, M.R.; Lazarus, A.J.; Mellott, M.M.; Parker, E.N.; Rosner, R.; Rottman, G.J.; Slavin, J.A.; Suess, S.T.; Tsurutani, B.T.; Woo, R.T.; Zwickl, R.D.

    1990-01-01

    The Solar Probe will deliver a 133.5 kg science payload into a 4 R s perihelion solar polar orbit (with the first perihelion passage in 2004) to explore in situ one of the last frontiers in the solar system---the solar corona. This mission is both affordable and technologically feasible. Using a payload of 12 (predominantly particles and fields) scientific experiments, it will be possible to answer many long-standing, fundamental problems concerning the structure and dynamics of the outer solar atmosphere, including the acceleration, storage, and transport of energetic particles near the Sun and in the inner ( s ) heliosphere

  4. Mobile Probing Kit

    DEFF Research Database (Denmark)

    Larsen, Jakob Eg; Sørensen, Lene Tolstrup; Sørensen, J.K.

    2007-01-01

    Mobile Probing Kit is a low tech and low cost methodology for obtaining inspiration and insights into user needs, requirements and ideas in the early phases of a system's development process. The methodology is developed to identify user needs, requirements and ideas among knowledge workers...... characterized as being highly nomadic and thus potential users of mobile and ubiquitous technologies. The methodology has been applied in the 1ST MAGNET Beyond project in order to obtain user needs and requirements in the process of developing pilot services. We report on the initial findings from applying...

  5. Test design requirements: Thermal conductivity probe testing

    International Nuclear Information System (INIS)

    Heath, R.E.

    1985-01-01

    This document establishes the test design requirements for development of a thermal conductivity probe test. The thermal conductivity probe determines in situ thermal conductivity using a line source transient heat conduction analysis. This document presents the rationale for thermal conductivity measurement using a thermal conductivity probe. A general test description is included. Support requirements along with design constraints are detailed to allow simple design of the thermal conductivity probe and test. The schedule and delivery requirements of the responsible test designer are also included. 7 refs., 1 fig

  6. Probing convex polygons with X-rays

    International Nuclear Information System (INIS)

    Edelsbrunner, H.; Skiena, S.S.

    1988-01-01

    An X-ray probe through a polygon measures the length of intersection between a line and the polygon. This paper considers the properties of various classes of X-ray probes, and shows how they interact to give finite strategies for completely describing convex n-gons. It is shown that (3n/2)+6 probes are sufficient to verify a specified n-gon, while for determining convex polygons (3n-1)/2 X-ray probes are necessary and 5n+O(1) sufficient, with 3n+O(1) sufficient given that a lower bound on the size of the smallest edge of P is known

  7. NASA's interstellar probe mission

    International Nuclear Information System (INIS)

    Liewer, P.C.; Ayon, J.A.; Wallace, R.A.; Mewaldt, R.A.

    2000-01-01

    NASA's Interstellar Probe will be the first spacecraft designed to explore the nearby interstellar medium and its interaction with our solar system. As envisioned by NASA's Interstellar Probe Science and Technology Definition Team, the spacecraft will be propelled by a solar sail to reach >200 AU in 15 years. Interstellar Probe will investigate how the Sun interacts with its environment and will directly measure the properties and composition of the dust, neutrals and plasma of the local interstellar material which surrounds the solar system. In the mission concept developed in the spring of 1999, a 400-m diameter solar sail accelerates the spacecraft to ∼15 AU/year, roughly 5 times the speed of Voyager 1 and 2. The sail is used to first bring the spacecraft to ∼0.25 AU to increase the radiation pressure before heading out in the interstellar upwind direction. After jettisoning the sail at ∼5 AU, the spacecraft coasts to 200-400 AU, exploring the Kuiper Belt, the boundaries of the heliosphere, and the nearby interstellar medium

  8. The in-line measurement of plant cell biomass using radio frequency impedance spectroscopy as a component of process analytical technology.

    Science.gov (United States)

    Holland, Tanja; Blessing, Daniel; Hellwig, Stephan; Sack, Markus

    2013-10-01

    Radio frequency impedance spectroscopy (RFIS) is a robust method for the determination of cell biomass during fermentation. RFIS allows non-invasive in-line monitoring of the passive electrical properties of cells in suspension and can distinguish between living and dead cells based on their distinct behavior in an applied radio frequency field. We used continuous in situ RFIS to monitor batch-cultivated plant suspension cell cultures in stirred-tank bioreactors and compared the in-line data to conventional off-line measurements. RFIS-based analysis was more rapid and more accurate than conventional biomass determination, and was sensitive to changes in cell viability. The higher resolution of the in-line measurement revealed subtle changes in cell growth which were not accessible using conventional methods. Thus, RFIS is well suited for correlating such changes with intracellular states and product accumulation, providing unique opportunities for employing systems biotechnology and process analytical technology approaches to increase product yield and quality. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Database architecture and query structures for probe data processing.

    Science.gov (United States)

    2012-03-01

    This report summarizes findings and implementations of probe vehicle data collection based on Bluetooth MAC address matching technology. Probe vehicle travel time data are studied in the following field deployment case studies: analysis of traffic ch...

  10. Characterization of chlorinated solvent contamination in limestone using innovative FLUTe® technologies in combination with other methods in a line of evidence approach

    DEFF Research Database (Denmark)

    Broholm, Mette Martina; Janniche, Gry Sander; Mosthaf, Klaus

    2016-01-01

    Characterization of dense non-aqueous phase liquid (DNAPL) source zones in limestone aquifers/bedrock is essential to develop accurate site-specific conceptual models and perform risk assessment. Here innovative field methods were combined to improve determination of source zone architecture......, hydrogeology and contaminant distribution. The FACT™ is a new technology and it was applied and tested at a contaminated site with a limestone aquifer, together with a number of existing methods including wire-line coring with core subsampling, FLUTe® transmissivity profiling and multilevel water sampling...... groundwater sampling (under two flow conditions) and FACT™ sampling and analysis combined with FLUTe® transmissivity profiling and modeling were used to provide a line of evidence for the presence of DNAPL, dissolved and sorbed phase contamination in the limestone fractures and matrix. The combined methods...

  11. Evaluation of some chemical and technological properties of induced erect chickpea mutant lines developed under drought stressed conditions

    International Nuclear Information System (INIS)

    Moustafa, R.A.K.; Ali, H.G.M.

    2009-01-01

    Seeds of the chickpea variety Flip 99-47 C were treated with gamma rays at doses of 0, 50 and 75 Gy and sown in the winter season of 2004/2005 to raise M1 generation under ordinary (normal) irrigation conditions. Bulked seeds from each treatment were planted in the subsequent growing seasons of 2005/2006 and 2006/2007 to advance M2 and M3 generations, respectively under either ordinary (normal) irrigation or drought stress condition. In the third generation, three erect mutant lines were derived from 75 Gy mutagenic treatment under drought stress compared to semi spreading growth habit of the initiated variety Flip 99-47 C. In the winter season of 2007/2008, M4 bulked seeds from the three erect lines as well as unirradiated seeds of the original variety grown under either ordinary (normal) irrigation (2152.5 m 3 /fad.) or drought (1159.2 m 3 /fad.) conditions were analyzed for the chemical composition and nutritional values. Obtained results indicated that there were slight decreases in protein and fat contents accompanied with marginal increases in both ash and carbohydrates in seed samples of the erect mutant developed under drought stress as compared to unirradiated seeds of the original variety grown under ordinary (normal) irrigation treatment. An opposite trend was noticed between seed samples derived from the erect lines compared to seeds of the parent variety developed under drought condition. Negligible changes in levels of the minerals (iron, magnesium, calcium and phosphorus) were detected between seeds of the erect lines and the original variety that grown under either ordinary (normal) irrigation or drought conditions. Cooking time (min) and hydration coefficient values did not much differ between the three tested seed samples. Marginal differences in essential and non-essential amino acids were detected between seeds of the erect mutants and those of the initial variety grown under ordinary (normal) irrigation or drought stressed conditions

  12. Technology of drill hole and well cleaning for in-situ leaching on the 16th section line ore body

    International Nuclear Information System (INIS)

    Li Xiqi; Liu Jianxiang; Wang Haifeng

    2003-01-01

    The specific feature of the 16th section line ore body of a deposit is deep water table and low confined aquifer. It causes the most trouble for well cleaning during completion and recovering the ore-bearing aquifer to natural state after it is worsened. It is a key point mining the ore body to search for a suitable way for well cleaning at the deep water table

  13. Gravity Probe B Inspection

    Science.gov (United States)

    2000-01-01

    The space vehicle Gravity Probe B (GP-B) is the relativity experiment developed at Stanford University to test two extraordinary predictions of Albert Einstein's general theory of relativity. The experiment will measure, very precisely, the expected tiny changes in the direction of the spin axes of four gyroscopes contained in an Earth-orbiting satellite at a 400-mile altitude. So free are the gyroscopes from disturbance that they will provide an almost perfect space-time reference system. They will measure how space and time are very slightly warped by the presence of the Earth, and, more profoundly, how the Earth's rotation very slightly drags space-time around with it. These effects, though small for the Earth, have far-reaching implications for the nature of matter and the structure of the Universe. GP-B is among the most thoroughly researched programs ever undertaken by NASA. This is the story of a scientific quest in which physicists and engineers have collaborated closely over many years. Inspired by their quest, they have invented a whole range of technologies that are already enlivening other branches of science and engineering. In this photograph, engineer Gary Reynolds is inspecting the inside of the probe neck during probe thermal repairs. GP-B is scheduled for launch in April 2004 and managed for NASA by the Marshall Space Flight Center. Development of the GP-B is the responsibility of Stanford University along with major subcontractor Lockheed Martin Corporation. (Image credit to Russ Leese, Gravity Probe B, Stanford University)

  14. Designing of a technological line in the context of controlling with the use of integration of the virtual controller with the mechatronics concept designer module of the PLM Siemens NX software

    Science.gov (United States)

    Herbuś, K.; Ociepka, P.

    2017-08-01

    In the work is examined the sequential control system of a technological line in the form of the final part of a system of an internal transport. The process of designing this technological line using the computer-aided approach ran concurrently in two different program environments. In the Mechatronics Concept Designer module of the PLM Siemens NX software was developed the 3D model of the technological line prepared for verification the logic interrelations implemented in the control system. For this purpose, from the whole system of the technological line, it was distinguished the sub-system of actuators and sensors, because their correct operation determines the correct operation of the whole system. Whereas in the application of the virtual controller have been implemented the algorithms of work of the planned line. Then both program environments have been integrated using the OPC server, which enables the exchange of data between the considered systems. The data on the state of the object and the data defining the way and sequence of operation of the technological line are exchanged between the virtual controller and the 3D model of the technological line in real time.

  15. NeuroMEMS: Neural Probe Microtechnologies

    Directory of Open Access Journals (Sweden)

    Sam Musallam

    2008-10-01

    Full Text Available Neural probe technologies have already had a significant positive effect on our understanding of the brain by revealing the functioning of networks of biological neurons. Probes are implanted in different areas of the brain to record and/or stimulate specific sites in the brain. Neural probes are currently used in many clinical settings for diagnosis of brain diseases such as seizers, epilepsy, migraine, Alzheimer’s, and dementia. We find these devices assisting paralyzed patients by allowing them to operate computers or robots using their neural activity. In recent years, probe technologies were assisted by rapid advancements in microfabrication and microelectronic technologies and thus are enabling highly functional and robust neural probes which are opening new and exciting avenues in neural sciences and brain machine interfaces. With a wide variety of probes that have been designed, fabricated, and tested to date, this review aims to provide an overview of the advances and recent progress in the microfabrication techniques of neural probes. In addition, we aim to highlight the challenges faced in developing and implementing ultralong multi-site recording probes that are needed to monitor neural activity from deeper regions in the brain. Finally, we review techniques that can improve the biocompatibility of the neural probes to minimize the immune response and encourage neural growth around the electrodes for long term implantation studies.

  16. Proximal Probes Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Proximal Probes Facility consists of laboratories for microscopy, spectroscopy, and probing of nanostructured materials and their functional properties. At the...

  17. Probe Techniques. Introductory Remarks

    Energy Technology Data Exchange (ETDEWEB)

    Emeleus, K. G. [School of Physics and Applied Mathematics, Queen' s University, Belfast (United Kingdom)

    1968-04-15

    In this brief introduction to the session on probes, the history of theii development is first touched on briefly. Reference is then made to the significance of the work to be described by Medicus, for conductivity and recombination calculations, and by Lam and Su, for a wide range of medium and higher pressure plasmas. Finally, a number of other probe topics are mentioned, including multiple probes; probes in electronegative plasmas; resonance probes; probes in noisy discharges; probes as oscillation detectors; use of probes where space-charge is not negligible. (author)

  18. On-line near infrared spectroscopy as a Process Analytical Technology (PAT) tool to control an industrial seeded API crystallization.

    Science.gov (United States)

    Schaefer, C; Lecomte, C; Clicq, D; Merschaert, A; Norrant, E; Fotiadu, F

    2013-09-01

    The final step of an active pharmaceutical ingredient (API) manufacturing synthesis process consists of a crystallization during which the API and residual solvent contents have to be quantified precisely in order to reach a predefined seeding point. A feasibility study was conducted to demonstrate the suitability of on-line NIR spectroscopy to control this step in line with new version of the European Medicines Agency (EMA) guideline [1]. A quantitative method was developed at laboratory scale using statistical design of experiments (DOE) and multivariate data analysis such as principal component analysis (PCA) and partial least squares (PLS) regression. NIR models were built to quantify the API in the range of 9-12% (w/w) and to quantify the residual methanol in the range of 0-3% (w/w). To improve the predictive ability of the models, the development procedure encompassed: outliers elimination, optimum model rank definition, spectral range and spectral pre-treatment selection. Conventional criteria such as, number of PLS factors, R(2), root mean square errors of calibration, cross-validation and prediction (RMSEC, RMSECV, RMSEP) enabled the selection of three model candidates. These models were tested in the industrial pilot plant during three technical campaigns. Results of the most suitable models were evaluated against to the chromatographic reference methods. Maximum relative bias of 2.88% was obtained about API target content. Absolute bias of 0.01 and 0.02% (w/w) respectively were achieved at methanol content levels of 0.10 and 0.13% (w/w). The repeatability was assessed as sufficient for the on-line monitoring of the 2 analytes. The present feasibility study confirmed the possibility to use on-line NIR spectroscopy as a PAT tool to monitor in real-time both the API and the residual methanol contents, in order to control the seeding of an API crystallization at industrial scale. Furthermore, the successful scale-up of the method proved its capability to be

  19. Removing celiac disease-related gluten proteins from bread wheat while retaining technological properties: a study with Chinese Spring deletion lines.

    Science.gov (United States)

    van den Broeck, Hetty C; van Herpen, Teun W J M; Schuit, Cees; Salentijn, Elma M J; Dekking, Liesbeth; Bosch, Dirk; Hamer, Rob J; Smulders, Marinus J M; Gilissen, Ludovicus J W J; van der Meer, Ingrid M

    2009-04-07

    Gluten proteins can induce celiac disease (CD) in genetically susceptible individuals. In CD patients gluten-derived peptides are presented to the immune system, which leads to a CD4+ T-cell mediated immune response and inflammation of the small intestine. However, not all gluten proteins contain T-cell stimulatory epitopes. Gluten proteins are encoded by multigene loci present on chromosomes 1 and 6 of the three different genomes of hexaploid bread wheat (Triticum aestivum) (AABBDD). The effects of deleting individual gluten loci on both the level of T-cell stimulatory epitopes in the gluten proteome and the technological properties of the flour were analyzed using a set of deletion lines of Triticum aestivum cv. Chinese Spring. The reduction of T-cell stimulatory epitopes was analyzed using monoclonal antibodies that recognize T-cell epitopes present in gluten proteins. The deletion lines were technologically tested with respect to dough mixing properties and dough rheology. The results show that removing the alpha-gliadin locus from the short arm of chromosome 6 of the D-genome (6DS) resulted in a significant decrease in the presence of T-cell stimulatory epitopes but also in a significant loss of technological properties. However, removing the omega-gliadin, gamma-gliadin, and LMW-GS loci from the short arm of chromosome 1 of the D-genome (1DS) removed T-cell stimulatory epitopes from the proteome while maintaining technological properties. The consequences of these data are discussed with regard to reducing the load of T-cell stimulatory epitopes in wheat, and to contributing to the design of CD-safe wheat varieties.

  20. Removing celiac disease-related gluten proteins from bread wheat while retaining technological properties: a study with Chinese Spring deletion lines

    Directory of Open Access Journals (Sweden)

    Bosch Dirk

    2009-04-01

    Full Text Available Abstract Background Gluten proteins can induce celiac disease (CD in genetically susceptible individuals. In CD patients gluten-derived peptides are presented to the immune system, which leads to a CD4+ T-cell mediated immune response and inflammation of the small intestine. However, not all gluten proteins contain T-cell stimulatory epitopes. Gluten proteins are encoded by multigene loci present on chromosomes 1 and 6 of the three different genomes of hexaploid bread wheat (Triticum aestivum (AABBDD. Results The effects of deleting individual gluten loci on both the level of T-cell stimulatory epitopes in the gluten proteome and the technological properties of the flour were analyzed using a set of deletion lines of Triticum aestivum cv. Chinese Spring. The reduction of T-cell stimulatory epitopes was analyzed using monoclonal antibodies that recognize T-cell epitopes present in gluten proteins. The deletion lines were technologically tested with respect to dough mixing properties and dough rheology. The results show that removing the α-gliadin locus from the short arm of chromosome 6 of the D-genome (6DS resulted in a significant decrease in the presence of T-cell stimulatory epitopes but also in a significant loss of technological properties. However, removing the ω-gliadin, γ-gliadin, and LMW-GS loci from the short arm of chromosome 1 of the D-genome (1DS removed T-cell stimulatory epitopes from the proteome while maintaining technological properties. Conclusion The consequences of these data are discussed with regard to reducing the load of T-cell stimulatory epitopes in wheat, and to contributing to the design of CD-safe wheat varieties.

  1. Free radical scavenging properties of some wine probes

    International Nuclear Information System (INIS)

    Stasko, A.; Liptakova, M.; Malik, F.

    1999-01-01

    There are preliminary results of investigation of scavenging properties of 8 probes of Slovak wines (consisting of one reference, 3 probes of white wine and 4 probes of red wine). According to the literature so far, wine probes contain paramagnetic species (Mn 2+ , characterised with sextet spectrum, and a singlet line around g=2,00). In our probes we observed Mn 2+ signals, but no significant evidence for a single line of free radical was found. We can conclude that Mn 2+ content in the red wines is generally higher than in the white ones. Further, we investigated the scavenging activities of the probes adding solution of dinitropicryl hydrazyl (DPPH-stable radical) to them. Their ability to terminate free radicals resulted in the decrease of the final DPPH concentrations in the probes. The red wines have significantly higher capability to scavenge free radicals than the probes of white wines. (authors)

  2. Remote tuning of NMR probe circuits.

    Science.gov (United States)

    Kodibagkar, V D; Conradi, M S

    2000-05-01

    There are many circumstances in which the probe tuning adjustments cannot be located near the rf NMR coil. These may occur in high-temperature NMR, low-temperature NMR, and in the use of magnets with small diameter access bores. We address here circuitry for connecting a fixed-tuned probe circuit by a transmission line to a remotely located tuning network. In particular, the bandwidth over which the probe may be remotely tuned while keeping the losses in the transmission line acceptably low is considered. The results show that for all resonant circuit geometries (series, parallel, series-parallel), overcoupling of the line to the tuned circuit is key to obtaining a large tuning bandwidth. At equivalent extents of overcoupling, all resonant circuit geometries have nearly equal remote tuning bandwidths. Particularly for the case of low-loss transmission line, the tuning bandwidth can be many times the tuned circuit's bandwidth, f(o)/Q. Copyright 2000 Academic Press.

  3. Information and Communication Technologies in the Improvement of the Legislative Process: Main Lines for a Legislative Process More Interactive

    Directory of Open Access Journals (Sweden)

    João Victor Rozatti Longhi

    2015-12-01

    Full Text Available Information and Communication Technologies (ICTs definitely transformed contemporary society. There are many behaviors that have changed under the massive use of such technologies, coming to public and private institutions and the law as a whole. In the formation of political will, in turn, ICTs have played increasingly important role. This work has as main objective to study the possibility of making true the intersection between technology and policy deliberation is a possible reality in Brazil. To do this, first, briefly reviews the study of two of these experiences, enshrined in several studies for its pioneering. The first concerns the Estonian reality of online electronic voting. The second is the Icelandic experience in 2011, known by the high degree of interactivity and colaborativity during the process. Finally, in the second part, we seek through a raid on democracy models see if Brazil is prepared to continue the evolution of its democratic model towards a greater degree of participation. And the main hypothesis is that interactivity can become rule through the legislative process.

  4. In line inspection of multi-diameter and high-pressure pipelines in Brazil using combined technologies: magnetic flux leakage and ultrasonic testing

    Energy Technology Data Exchange (ETDEWEB)

    Ginten, Markus; Brockhaus, Stephan; Bouaoua, Nourreddine; Klein, Stefan [ROSEN Technology and Research Center, Lingen (Germany); Bruening, Franz [ROSEN Brazil, Rio de Janeiro, RJ (Brazil)

    2009-07-01

    The simultaneous use of the magnetic flux leakage (MFL) method and the ultrasonic testing (UT) method on a single in line inspection (ILI) tool has been identified as a versatile and accurate solution for liquid pipelines. The combination of the two methods is complementary to the restrictions of each other. Also, the overall scope of the inspection is enlarged. General wall thinning and largely corroded areas are accurately and reliably scanned with the UT unit, while very detailed information about pitting corrosion is obtained from the MFL measurement. Blind spots of echo loss, as occasionally observed for the UT channels is compensated by the more robust measurement from the MFL sensors. Consequently, this technology has been the method of choice in an in line inspection project of an onshore long distance pipeline in Brazil, facing a variety of corrosion threats. The pipeline consists of several multi-diameter sections of 18/20 inches and 20/22 inches. Furthermore, the high gravity of product in combination with a height profile, an altitude of 1152 m MSL (Mean Sea Level) had to be crossed, leads to a maximum pressure of 220 bar. These boundary conditions had to be considered during the design of the ILI-tool. The paper discusses the experience made so far with the combined technology MFL and UT. The effective use of the inspection tool for the above mentioned pipeline as well as field results from a previous inspection are described. (author)

  5. Scanning microscopic four-point conductivity probes

    DEFF Research Database (Denmark)

    Petersen, Christian Leth; Hansen, Torben Mikael; Bøggild, Peter

    2002-01-01

    A method for fabricating microscopic four-point probes is presented. The method uses silicon-based microfabrication technology involving only two patterning steps. The last step in the fabrication process is an unmasked deposition of the conducting probe material, and it is thus possible to select...... the conducting material either for a silicon wafer or a single probe unit. Using shadow masking photolithography an electrode spacing (pitch) down to 1.1 mum was obtained, with cantilever separation down to 200 run. Characterisation measurements have shown the microscopic probes to be mechanically very flexible...

  6. LookOnX: A new technological approach for the H1 off-line event display

    International Nuclear Information System (INIS)

    Perus, A.; Arnault, C.

    1994-01-01

    The authors present the re-engineering work achieved on a traditional-fashioned GKS graphical package with new open-quotes event drivenclose quotes concepts required by using workstation interactivity. The Off-line Event Display for the H1 experiment H1look was originally written using a basic kernel package - Look - that provides a uniform control engine for graphical modules based on conventional GKS graphics. The use of powerful UNIX workstations is growing daily and their new graphical functionalities and CPU power are well suited to interactive graphical data analysis. Therefore re-engineering work was needed to put in some new features such as: interactive manipulations of graphical objects; object oriented definitions of the graphical representations and connectivity between graphical objects and physical objects; multi-windowing facility

  7. Petroleum technologies: recent and future evolutions. Consequences on the gas production line; Technologies petrolieres: evolutions recentes et futures. Consequences sur la chaine gaz

    Energy Technology Data Exchange (ETDEWEB)

    Freud, E. [Institut Francais du Petrole, 92 - Rueil-Malmaison (France)

    1998-03-01

    This paper describes how recent technological progresses should solve the difficulties encountered in the exploitation of natural gas fields in hard environments (far away or isolated fields, deep offshore, small or complex fields, production requiring a complex processing). These techniques concern: the exploration of fields and reservoirs, the deep-sea drilling and production (poly-phase pumping, hydrates formation control), the processing of crudes (water/oil and oil/gas separation, dehydration, de-acidification, removal of impurities), the transport (gas-pipelines, LNG and chemical conversion). (J.S.)

  8. The Galaxy Evolution Probe

    Science.gov (United States)

    Glenn, Jason; Galaxy Evolution Probe Team

    2018-01-01

    The Galaxy Evolution Probe (GEP) is a concept for a far-infrared observatory to survey large regions of sky for star-forming galaxies from z = 0 to beyond z = 3. Our knowledge of galaxy formation is incomplete and requires uniform surveys over a large range of redshifts and environments to accurately describe mass assembly, star formation, supermassive black hole growth, interactions between these processes, and what led to their decline from z ~ 2 to the present day. Infrared observations are sensitive to dusty, star-forming galaxies, which have bright polycyclic aromatic hydrocarbon (PAH) emission features and warm dust continuum in the rest-frame mid infrared and cooler thermal dust emission in the far infrared. Unlike previous far-infrared continuum surveys, the GEP will measure photometric redshifts commensurate with galaxy detections from PAH emission and Si absorption features, without the need for obtaining spectroscopic redshifts of faint counterparts at other wavelengths.The GEP design includes a 2 m diameter telescope actively cooled to 4 K and two instruments: (1) An imager covering 10 to 300 um with 25 spectral resolution R ~ 8 bands (with lower R at the longest wavelengths) to detect star-forming galaxies and measure their redshifts photometrically. (2) A 23 – 190 um, R ~ 250 dispersive spectrometer for redshift confirmation and identification of obscured AGN using atomic fine-structure lines. Lines including [Ne V], [O IV], [O III], [O I], and [C II] will probe gas physical conditions, radiation field hardness, and metallicity. Notionally, the GEP will have a two-year mission: galaxy surveys with photometric redshifts in the first year and a second year devoted to follow-up spectroscopy. A comprehensive picture of star formation in galaxies over the last 10 billion years will be assembled from cosmologically relevant volumes, spanning environments from field galaxies and groups, to protoclusters, to dense galaxy clusters.Commissioned by NASA, the

  9. Site-Directed Genome Knockout in Chicken Cell Line and Embryos Can Use CRISPR/Cas Gene Editing Technology

    Directory of Open Access Journals (Sweden)

    Qisheng Zuo

    2016-06-01

    Full Text Available The present study established an efficient genome editing approach for the construction of stable transgenic cell lines of the domestic chicken (Gallus gallus domesticus. Our objectives were to facilitate the breeding of high-yield, high-quality chicken strains, and to investigate gene function in chicken stem cells. Three guide RNA (gRNAs were designed to knockout the C2EIP gene, and knockout efficiency was evaluated in DF-1 chicken fibroblasts and chicken ESCs using the luciferase single-strand annealing (SSA recombination assay, T7 endonuclease I (T7EI assay, and TA clone sequencing. In addition, the polyethylenimine-encapsulated Cas9/gRNA plasmid was injected into fresh fertilized eggs. At 4.5 d later, frozen sections of the embryos were prepared, and knockout efficiency was evaluated by the T7EI assay. SSA assay results showed that luciferase activity of the vector expressing gRNA-3 was double that of the control. Results of the T7EI assay and TA clone sequencing indicated that Cas9/gRNA vector-mediated gene knockdown efficiency was approximately 27% in both DF-1 cells and ESCs. The CRISPR/Cas9 vector was also expressed in chicken embryos, resulting in gene knockdown in three of the 20 embryos (gene knockdown efficiency 15%. Taken together, our results indicate that the CRISPR/Cas9 system can mediate stable gene knockdown at the cell and embryo levels in domestic chickens.

  10. Child protection and new technologies of communication: the code of regulatory PEGI videogames and games on-line

    Directory of Open Access Journals (Sweden)

    Petra Mª PÉREZ ALONSO-GETA

    2017-07-01

    Full Text Available It’s been said that the future of a village resides in its children, and not only because they’re the future, but because it’s better if we protect and teach our children well. Thus, in 1959 the United Nations published ten principles in the Declaration of the Rights of the Child. The second principle of the Declaration of the Rights states that “the child shall enjoy special protection…to enable him to develop physically, mentally, morally, spiritually and socially…” Today, a very different social context from that of 1959, the right to protection should also be settled within the diverse areas that define new communication technologies. However, current procedures established by the PEGI, while still necessary, don’t guarantee this basic childhood right.

  11. Technology line and case analysis of heat metering and energy efficiency retrofit of existing residential buildings in Northern heating areas of China

    International Nuclear Information System (INIS)

    Zhao Jing; Zhu Neng; Wu Yong

    2009-01-01

    The building area in northern heating areas accounting for 70% of the total land area in China is 6,500,000,000 m 2 . The average heating energy consumption in northern China is 100-200% times more than developed countries in the same latitude. This paper introduced firstly the heat metering and energy efficiency retrofit background of existing residential buildings in northern heating areas of China organized by mohurd and MOF, and then put forward the total principle and contents of retrofit. Through analyzing some retrofit cases in Germany, Poland and China, some technological experiences were summarized and finally a technology line suitable for heat metering and energy efficiency retrofit of existing residential buildings in northern heating areas of China which involved retrofit for heat metering and temperature regulation of heating systems, heat balance of heat source and network, and building envelope was described to provide a systematic, scientific, technological guide for the retrofit projects of 0.15 billion m 2 in 'the Eleventh Five-Year Plan' period.

  12. Diagnostic tools based on minor groove binder probe technology for rapid identification of vaccinal and field strains of canine parvovirus type 2b.

    Science.gov (United States)

    Decaro, Nicola; Martella, Vito; Elia, Gabriella; Desario, Costantina; Campolo, Marco; Buonavoglia, Domenico; Bellacicco, Anna Lucia; Tempesta, Maria; Buonavoglia, Canio

    2006-12-01

    TaqMan-based diagnostic tests have been developed for the identification of canine parvovirus type 2 (CPV-2) strains in the faeces of dogs with diarrhoea, including a minor groove binder (MGB) probe assay for identification of type 2-based vaccines and field strains (types 2a, 2b and 2c). Since type 2b vaccines have been licensed recently in Europe, two novel MGB assays were developed for discrimination between type 2b vaccines and field strains of CPV. Such assays have been found to be highly sensitive, specific and reproducible, allowing for simultaneous detection of type 2b vaccinal and field strains present in the same specimens. These new assays will help resolution of the diagnostic problems related to the detection of a type 2b strain in the faeces of dogs shortly after the administration of a type 2b vaccine.

  13. Achievement report on the research on possibility of expanding application of information technology to automobile production line; Jidosha seisan kotei eno joho tsushin gijutsu tekiyo kakudai kanosei ni kansuru chosa kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Before submitting propositions on important technological tasks to be generated in the automobile production line upon application of IT (information technology), a questionnairing is implemented to know the actualities of the line for the purpose of planning a proper merger of IT and the manufacturing technology development capability of the IT accepting site. The questionnaire, aiming at the embodiment of the goals of the projects 'IT related technical tasks relative to its helping human creation of new technologies and enhancement of human ability to make improvement' and 'Technical tasks of developing technical skills into technologies,' asks engineers about the actualities of knowhow at automobile production lines and its development into technologies, about how they take care of what they learned from their predecessors, and about their expectations from and views and opinions toward IT introduction and utilization. It is important to efficiently utilize IT as a means to solve problems involving the knowhow related training on the production line and the handing down of the acquired knowhow to the juniors. Since individual workers' knowhow assumes a certain role at each stage of the automobile production line and since it is necessary to extract the maximum from each worker and to utilize the fruit for improvement on productivity and work efficiency, it is indispensable to efficiently merge the knowhow with IT. (NEDO)

  14. Nanomaterials and MRI molecular probe

    International Nuclear Information System (INIS)

    Inubushi, Toshiro

    2008-01-01

    This paper presents the current state and future prospect of enhancing probes in MRI which enable to image specific cells and molecules mainly from the aspect of cell trafficking. Although MRI requires such probes for specific imaging, it has an advantage that anatomical images are simultaneously available even during surgical operation without radiation exposure, differing from X-CT, -transillumination and positron emission tomography (PET). In the development of novel MRI molecular probes, the recent topic concerns the cell trafficking biology where cells related with transplantation and immunological therapy can be traced. Although superparamagnetic iron oxide (SPIO) has been used as a commercially available enhancer, this nanoparticle has problems like a difficulty to penetrate cell, cytotoxicity and others. For these, authors have developed the nanoparticle SPIO covered with silica shell, which can be chemically modified, e.g., by binding fluorescent pigments to possibly allow MR bimodal molecular imaging. For penetration of particles in cells, envelop of Sendai virus is used. PET-CT has been more popular these days; however, MRI is superior to CT for imaging soft tissues, and development of PET-MRI is actively under progress aiming the multi-modal imaging. At present, molecular probes for MRI are certainly not so many as those for PET and cooperative efforts to develop the probes are required in medical, technological and pharmaceutical fields. (R.T.)

  15. EDITORIAL: Probing the nanoworld Probing the nanoworld

    Science.gov (United States)

    Miles, Mervyn

    2009-10-01

    In nanotechnology, it is the unique properties arising from nanometre-scale structures that lead not only to their technological importance but also to a better understanding of the underlying science. Over the last twenty years, material properties at the nanoscale have been dominated by the properties of carbon in the form of the C60 molecule, single- and multi-wall carbon nanotubes, nanodiamonds, and recently graphene. During this period, research published in the journal Nanotechnology has revealed the amazing mechanical properties of such materials as well as their remarkable electronic properties with the promise of new devices. Furthermore, nanoparticles, nanotubes, nanorods, and nanowires from metals and dielectrics have been characterized for their electronic, mechanical, optical, chemical and catalytic properties. Scanning probe microscopy (SPM) has become the main characterization technique and atomic force microscopy (AFM) the most frequently used SPM. Over the past twenty years, SPM techniques that were previously experimental in nature have become routine. At the same time, investigations using AFM continue to yield impressive results that demonstrate the great potential of this powerful imaging tool, particularly in close to physiological conditions. In this special issue a collaboration of researchers in Europe report the use of AFM to provide high-resolution topographical images of individual carbon nanotubes immobilized on various biological membranes, including a nuclear membrane for the first time (Lamprecht C et al 2009 Nanotechnology 20 434001). Other SPM developments such as high-speed AFM appear to be making a transition from specialist laboratories to the mainstream, and perhaps the same may be said for non-contact AFM. Looking to the future, characterisation techniques involving SPM and spectroscopy, such as tip-enhanced Raman spectroscopy, could emerge as everyday methods. In all these advanced techniques, routinely available probes will

  16. Scanning probe lithography for nanoimprinting mould fabrication

    International Nuclear Information System (INIS)

    Luo Gang; Xie Guoyong; Zhang Yongyi; Zhang Guoming; Zhang Yingying; Carlberg, Patrick; Zhu Tao; Liu Zhongfan

    2006-01-01

    We propose a rational fabrication method for nanoimprinting moulds by scanning probe lithography. By wet chemical etching, different kinds of moulds are realized on Si(110) and Si(100) surfaces according to the Si crystalline orientation. The structures have line widths of about 200 nm with a high aspect ratio. By reactive ion etching, moulds with patterns free from the limitation of Si crystalline orientation are also obtained. With closed-loop scan control of a scanning probe microscope, the length of patterned lines is more than 100 μm by integrating several steps of patterning. The fabrication process is optimized in order to produce a mould pattern with a line width about 10 nm. The structures on the mould are further duplicated into PMMA resists through the nanoimprinting process. The method of combining scanning probe lithography with wet chemical etching or reactive ion etching (RIE) provides a resistless route for the fabrication of nanoimprinting moulds

  17. On-line training in radiological protection by means of the designing and adaptation of a technological platform

    Energy Technology Data Exchange (ETDEWEB)

    Soto, M.; Campayo, J. M. [Logistica y Acondicionamientos Industriales SAU, Sorolla Center, local 10, Av. de las Cortes Valencianas No. 58, 46015 Valencia (Spain); Mayo, P. [TITANIA Servicios Tecnologicos SL, Sorolla Center, local 10, Av. de las Cortes Valencianas No. 58, 46015 Valencia (Spain); Verdu, G., E-mail: m.soto@lainsa.co [ISIRYM, Universidad Politecnica de Valencia, Camino de Vera s/n, Valencia (Spain)

    2010-10-15

    The companies that are working in maintenance, decontamination, dismantling and assessment in nuclear power plants, usually have their employees in different facilities far from its central offices. When there is a training in radiological protection applied to the nuclear field, it is difficult for these people the attendance to the course because of different reasons. One of them is the location of the formation centres which sometimes are not near from the nuclear facilities, so it is necessary the displacement of the employees, and the time in displacements to the formation centre and costs increase exponentially. In addition if they go to the courses they usually cannot attend their daily work with the same effectiveness depending on the duration of the course. In this work we present a technological platform that is designed in collaboration with the university of give different courses related with radiological field applied to nuclear and radioactive facilities. This platform is adapted and designed to different contents depending on the course. At the main screen we have included and index and announces of the course, so we have arranged the contents in different areas and sections. When the student finishes an area, an evaluation has to be done to prove the understanding of the lessons. We have included films of different activities as decontamination devices, radiation detectors, etc. with the contents to make the explanations more understandable to the student. The student can access to this platform with private password and follow the course individually. The course is complemented with a final review and exam that are not online to guarantee that the training is well finished. This type of training is more flexitime and can be adapted to the necessities of each user, avoiding high costs and unnecessary displacements. (Author)

  18. On-line training in radiological protection by means of the designing and adaptation of a technological platform

    International Nuclear Information System (INIS)

    Soto, M.; Campayo, J. M.; Mayo, P.; Verdu, G.

    2010-10-01

    The companies that are working in maintenance, decontamination, dismantling and assessment in nuclear power plants, usually have their employees in different facilities far from its central offices. When there is a training in radiological protection applied to the nuclear field, it is difficult for these people the attendance to the course because of different reasons. One of them is the location of the formation centres which sometimes are not near from the nuclear facilities, so it is necessary the displacement of the employees, and the time in displacements to the formation centre and costs increase exponentially. In addition if they go to the courses they usually cannot attend their daily work with the same effectiveness depending on the duration of the course. In this work we present a technological platform that is designed in collaboration with the university of give different courses related with radiological field applied to nuclear and radioactive facilities. This platform is adapted and designed to different contents depending on the course. At the main screen we have included and index and announces of the course, so we have arranged the contents in different areas and sections. When the student finishes an area, an evaluation has to be done to prove the understanding of the lessons. We have included films of different activities as decontamination devices, radiation detectors, etc. with the contents to make the explanations more understandable to the student. The student can access to this platform with private password and follow the course individually. The course is complemented with a final review and exam that are not online to guarantee that the training is well finished. This type of training is more flexitime and can be adapted to the necessities of each user, avoiding high costs and unnecessary displacements. (Author)

  19. Application of Micropore Filter Technology: Exploring the Blood Flow Path in Arterial-Line Filters and Its Effect on Bubble Trapping Functions.

    Science.gov (United States)

    Herbst, Daniel P

    2017-03-01

    Conventional arterial-line filters commonly use a large volume circular shaped housing, a wetted micropore screen, and a purge port to trap, separate, and remove gas bubbles from extracorporeal blood flow. Focusing on the bubble trapping function, this work attempts to explore how the filter housing shape and its resulting blood flow path affect the clinical application of arterial-line filters in terms of gross air handling. A video camera was used in a wet-lab setting to record observations made during gross air-bolus injections in three different radially designed filters using a 30-70% glycerol-saline mixture flowing at 4.5 L/min. Two of the filters both had inlet ports attached near the filter-housing top with bottom oriented outlet ports at the bottom, whereas the third filter had its inlet and outlet ports both located at the bottom of the filter housing. The two filters with top-in bottom-out fluid paths were shown to direct the incoming flow downward as it passed through the filter, placing the forces of buoyancy and viscous drag in opposition to each other. This contrasted with the third filter's bottom-in bottom-out fluid path, which was shown to direct the incoming flow upward so that the forces of buoyancy and viscous drag work together. The direction of the blood flow path through a filter may be important to the application of arterial-line filter technology as it helps determine how the forces of buoyancy and flow are aligned with one another.

  20. Automatic on-line detection system design research on internal defects of metal materials based on optical fiber F-P sensing technology

    Science.gov (United States)

    Xia, Liu; Shan, Ning; Chao, Ban; Caoshan, Wang

    2016-10-01

    Metal materials have been used in aerospace and other industrial fields widely because of its excellent characteristics, so its internal defects detection is very important. Ultrasound technology is used widely in the fields of nondestructive detection because of its excellent characteristic. But the conventional detection instrument for ultrasound, which has shortcomings such as low intelligent level and long development cycles, limits its development. In this paper, the theory of ultrasound detection is analyzed. A computational method of the defects distributional position is given. The non-contact type optical fiber F-P interference cavity structure is designed and the length of origin cavity is given. The real-time on-line ultrasound detecting experiment devices for internal defects of metal materials is established based on the optical fiber F-P sensing system. The virtual instrument of automation ultrasound detection internal defects is developed based on LabVIEW software and the experimental study is carried out. The results show that this system can be used in internal defect real-time on-line locating of engineering structures effectively. This system has higher measurement precision. Relative error is 6.7%. It can be met the requirement of engineering practice. The system is characterized by simple operation, easy realization. The software has a friendly interface, good expansibility, and high intelligent level.

  1. Mobile Game Probes

    DEFF Research Database (Denmark)

    Borup Lynggaard, Aviaja

    2006-01-01

    This paper will examine how probes can be useful for game designers in the preliminary phases of a design process. The work is based upon a case study concerning pervasive mobile phone games where Mobile Game Probes have emerged from the project. The new probes are aimed towards a specific target...... group and the goal is to specify the probes so they will cover the most relevant areas for our project. The Mobile Game Probes generated many interesting results and new issues occurred, since the probes came to be dynamic and favorable for the process in new ways....

  2. Directional rf probe for measurement of conductivity of flowing plasmas

    International Nuclear Information System (INIS)

    Jayakumar, R.; Chakravarthy, D.P.; Rohatgi, V.K.

    1977-01-01

    An electrodeless immersible rf probe for measurement of plasma conductivity in the range 0.01 to 100 mho/m has been designed and fabricated. The probe, with an overall diameter of 11 mm, employs unidirectional electromagnetic field lines which reduce the inaccuracies caused by insertion of the probe in a flowing plasma. In the range studied the probe output shows a linear relationship with the conductivity of the medium. Such probes are of interest in the study of MHD and reentry plasmas

  3. Parallel Lines

    Directory of Open Access Journals (Sweden)

    James G. Worner

    2017-05-01

    Full Text Available James Worner is an Australian-based writer and scholar currently pursuing a PhD at the University of Technology Sydney. His research seeks to expose masculinities lost in the shadow of Australia’s Anzac hegemony while exploring new opportunities for contemporary historiography. He is the recipient of the Doctoral Scholarship in Historical Consciousness at the university’s Australian Centre of Public History and will be hosted by the University of Bologna during 2017 on a doctoral research writing scholarship.   ‘Parallel Lines’ is one of a collection of stories, The Shapes of Us, exploring liminal spaces of modern life: class, gender, sexuality, race, religion and education. It looks at lives, like lines, that do not meet but which travel in proximity, simultaneously attracted and repelled. James’ short stories have been published in various journals and anthologies.

  4. Molecular Imaging Probe Development using Microfluidics

    Science.gov (United States)

    Liu, Kan; Wang, Ming-Wei; Lin, Wei-Yu; Phung, Duy Linh; Girgis, Mark D.; Wu, Anna M.; Tomlinson, James S.; Shen, Clifton K.-F.

    2012-01-01

    In this manuscript, we review the latest advancement of microfluidics in molecular imaging probe development. Due to increasing needs for medical imaging, high demand for many types of molecular imaging probes will have to be met by exploiting novel chemistry/radiochemistry and engineering technologies to improve the production and development of suitable probes. The microfluidic-based probe synthesis is currently attracting a great deal of interest because of their potential to deliver many advantages over conventional systems. Numerous chemical reactions have been successfully performed in micro-reactors and the results convincingly demonstrate with great benefits to aid synthetic procedures, such as purer products, higher yields, shorter reaction times compared to the corresponding batch/macroscale reactions, and more benign reaction conditions. Several ‘proof-of-principle’ examples of molecular imaging probe syntheses using microfluidics, along with basics of device architecture and operation, and their potential limitations are discussed here. PMID:22977436

  5. Probe-diverse ptychography

    Energy Technology Data Exchange (ETDEWEB)

    Peterson, I., E-mail: isaac.russellpeterson@rmit.edu.au [ARC Centre of Excellence for Coherent X-ray Science, the University of Melbourne, School of Physics, Victoria 3010 (Australia); Harder, R. [Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439 (United States); Robinson, I.K. [Research Complex at Harwell, Didcot, Oxfordshire OX11 0DE (United Kingdom); London Centre for Nanotechnology, University College London, London WC1H 0AH (United Kingdom)

    2016-12-15

    We propose an extension of ptychography where the target sample is scanned separately through several probes with distinct amplitude and phase profiles and a diffraction image is recorded for each probe and each sample translation. The resulting probe-diverse dataset is used to iteratively retrieve high-resolution images of the sample and all probes simultaneously. The method is shown to yield significant improvement in the reconstructed sample image compared to the image obtained using the standard single-probe ptychographic phase-retrieval scheme.

  6. Traversing probe system

    International Nuclear Information System (INIS)

    Mashburn, D.N.; Stevens, R.H.; Woodall, H.C.

    1977-01-01

    This invention comprises a rotatable annular probe-positioner which carries at least one radially disposed sensing probe, such as a Pitot tube having a right-angled tip. The positioner can be coaxially and rotatably mounted within a compressor casing or the like and then actuated to orient the sensing probe as required to make measurements at selected stations in the annulus between the positioner and compressor casing. The positioner can be actuated to (a) selectively move the probe along its own axis, (b) adjust the yaw angle of the right-angled probe tip, and (c) revolve the probe about the axis common to the positioner and casing. A cam plate engages a cam-follower portion of the probe and normally rotates with the positioner. The positioner includes a first-motor-driven ring gear which effects slidable movement of the probe by rotating the positioner at a time when an external pneumatic cylinder is actuated to engage the cam plate and hold it stationary. When the pneumatic cylinder is not actuated, this ring gear can be driven to revolve the positioner and thus the probe to a desired circumferential location about the above-mentioned common axis. A second motor-driven ring gear included in the positioner can be driven to rotate the probe about its axis, thus adjusting the yaw angle of the probe tip. The positioner can be used in highly corrosive atmosphere, such as gaseous uranium hexafluoride. 10 claims, 6 figures

  7. Traversing probe system

    Science.gov (United States)

    Mashburn, Douglas N.; Stevens, Richard H.; Woodall, Harold C.

    1977-01-01

    This invention comprises a rotatable annular probe-positioner which carries at least one radially disposed sensing probe, such as a Pitot tube having a right-angled tip. The positioner can be coaxially and rotatably mounted within a compressor casing or the like and then actuated to orient the sensing probe as required to make measurements at selected stations in the annulus between the positioner and compressor casing. The positioner can be actuated to (a) selectively move the probe along its own axis, (b) adjust the yaw angle of the right-angled probe tip, and (c) revolve the probe about the axis common to the positioner and casing. A cam plate engages a cam-follower portion of the probe and normally rotates with the positioner. The positioner includes a first-motor-driven ring gear which effects slidable movement of the probe by rotating the positioner at a time when an external pneumatic cylinder is actuated to engage the cam plate and hold it stationary. When the pneumatic cylinder is not actuated, this ring gear can be driven to revolve the positioner and thus the probe to a desired circumferential location about the above-mentioned common axis. A second motor-driven ring gear included in the positioner can be driven to rotate the probe about its axis, thus adjusting the yaw angle of the probe tip. The positioner can be used in highly corrosive atmosphere, such as gaseous uranium hexafluoride.

  8. Electrical resistivity probes

    Science.gov (United States)

    Lee, Ki Ha; Becker, Alex; Faybishenko, Boris A.; Solbau, Ray D.

    2003-10-21

    A miniaturized electrical resistivity (ER) probe based on a known current-voltage (I-V) electrode structure, the Wenner array, is designed for local (point) measurement. A pair of voltage measuring electrodes are positioned between a pair of current carrying electrodes. The electrodes are typically about 1 cm long, separated by 1 cm, so the probe is only about 1 inch long. The electrodes are mounted to a rigid tube with electrical wires in the tube and a sand bag may be placed around the electrodes to protect the electrodes. The probes can be positioned in a borehole or on the surface. The electrodes make contact with the surrounding medium. In a dual mode system, individual probes of a plurality of spaced probes can be used to measure local resistance, i.e. point measurements, but the system can select different probes to make interval measurements between probes and between boreholes.

  9. NanoDLSay: a new platform technology for biomolecular detection and analysis using gold nanoparticle probes coupled with dynamic light scattering

    Science.gov (United States)

    Bogdanovic, Jelena; Huo, Qun

    2010-04-01

    Most analytical techniques that are routinely used in biomedical research for detection and quantification of biomolecules are time-consuming, expensive and labor-intensive, and there is always a need for rapid, affordable and convenient methods. Recently we have developed a new platform technology for biomolecular detection and analysis: NanoDLSay. NanoDLSay employs antibody-coated gold nanoparticles (GNPs) and dynamic light scattering, and correlates the specific increase in particle size after antigen-antibody interaction to the target antigen concentration. We applied this technology to develop an assay for rapid detection of actin, a protein widely used as a loading control in Western Blot analysis. GNPs were coated with two types of polyclonal anti-actin antibodies, and used in the assay to detect two types of actin: β- and bovine skeletal muscle actin in RIPA buffer. The results of our study revealed some complex aspects of actin binding characteristics, which depended on the type of actin reagent and anti-actin antibody used. A surprising finding was a reverse dose-response relationship between the actin concentration and the average particle size in the assay solution, which we attributed to the effect of RIPA buffer. Our results indicate that RIPA may also interfere in other types of nanoparticle-based assays, and that this interference deserves further study.

  10. Technology.

    Science.gov (United States)

    Online-Offline, 1998

    1998-01-01

    Focuses on technology, on advances in such areas as aeronautics, electronics, physics, the space sciences, as well as computers and the attendant progress in medicine, robotics, and artificial intelligence. Describes educational resources for elementary and middle school students, including Web sites, CD-ROMs and software, videotapes, books,…

  11. NASA SMART Probe: Breast Cancer Application

    Science.gov (United States)

    Mah, Robert W.; Norvig, Peter (Technical Monitor)

    2000-01-01

    There is evidence in breast cancer and other malignancies that the physiologic environment within a tumor correlates with clinical outcome. We are developing a unique percutaneous Smart Probe to be used at the time of needle biopsy of the breast. The Smart Probe will simultaneously measure multiple physiologic parameters within a breast tumor. Direct and indirect measurements of tissue oxygen levels, blood flow, pH, and tissue fluid pressure will be analyzed in real-time. These parameters will be interpreted individually and collectively by innovative neural network techniques using advanced intelligent software. The goals are 1) develop a pecutaneous Smart Probe with multiple sensor modalities and applying advanced Information Technologies to provide real time diagnostic information of the tissue at tip of the probe, 2) test the percutaneous Smart Probe in women with benign and malignant breast masses who will be undergoing surgical biopsy, 3) correlate probe sensor data with benign and malignant status of breast masses, 4) determine whether the probe can detect physiologic differences within a breast tumor, and its margins, and in adjacent normal breast tissue, 5) correlate probe sensor data with known prognostic factors for breast caner, including tumor size, tumor grade, axillary lymph node metastases, estrogen receptor and progesterone receptor status.

  12. Primordial Germ Cell-Mediated Chimera Technology Produces Viable Pure-Line Houbara Bustard Offspring: Potential for Repopulating an Endangered Species

    Science.gov (United States)

    Wernery, Ulrich; Liu, Chunhai; Baskar, Vijay; Guerineche, Zhor; Khazanehdari, Kamal A.; Saleem, Shazia; Kinne, Jörg; Wernery, Renate

    2010-01-01

    Background The Houbara bustard (Chlamydotis undulata) is a wild seasonal breeding bird populating arid sandy semi-desert habitats in North Africa and the Middle East. Its population has declined drastically during the last two decades and it is classified as vulnerable. Captive breeding programmes have, hitherto, been unsuccessful in reviving population numbers and thus radical technological solutions are essential for the long term survival of this species. The purpose of this study was to investigate the use of primordial germ cell-mediated chimera technology to produce viable Houbara bustard offspring. Methodology/Principal Findings Embryonic gonadal tissue was dissected from Houbara bustard embryos at eight days post-incubation. Subsequently, Houbara tissue containing gonadal primordial germ cells (gPGCs) was injected into White Leghorn chicken (Gallus gallus domesticus) embryos, producing 83/138 surviving male chimeric embryos, of which 35 chimeric roosters reached sexual maturity after 5 months. The incorporation and differentiation of Houbara gPGCs in chimeric chicken testis were assessed by PCR with Houbara-specific primers and 31.3% (5/16) gonads collected from the injected chicken embryos showed the presence of donor Houbara cells. A total of 302 semen samples from 34 chimeric roosters were analyzed and eight were confirmed as germline chimeras. Semen samples from these eight roosters were used to artificially inseminate three female Houbara bustards. Subsequently, 45 Houbara eggs were obtained and incubated, two of which were fertile. One egg hatched as a male live born Houbara; the other was female but died before hatching. Genotyping confirmed that the male chick was a pure-line Houbara derived from a chimeric rooster. Conclusion This study demonstrates for the first time that Houbara gPGCs can migrate, differentiate and eventually give rise to functional sperm in the chimeric chicken testis. This approach may provide a promising tool for propagation

  13. Primordial germ cell-mediated chimera technology produces viable pure-line Houbara bustard offspring: potential for repopulating an endangered species.

    Directory of Open Access Journals (Sweden)

    Ulrich Wernery

    2010-12-01

    Full Text Available The Houbara bustard (Chlamydotis undulata is a wild seasonal breeding bird populating arid sandy semi-desert habitats in North Africa and the Middle East. Its population has declined drastically during the last two decades and it is classified as vulnerable. Captive breeding programmes have, hitherto, been unsuccessful in reviving population numbers and thus radical technological solutions are essential for the long term survival of this species. The purpose of this study was to investigate the use of primordial germ cell-mediated chimera technology to produce viable Houbara bustard offspring.Embryonic gonadal tissue was dissected from Houbara bustard embryos at eight days post-incubation. Subsequently, Houbara tissue containing gonadal primordial germ cells (gPGCs was injected into White Leghorn chicken (Gallus gallus domesticus embryos, producing 83/138 surviving male chimeric embryos, of which 35 chimeric roosters reached sexual maturity after 5 months. The incorporation and differentiation of Houbara gPGCs in chimeric chicken testis were assessed by PCR with Houbara-specific primers and 31.3% (5/16 gonads collected from the injected chicken embryos showed the presence of donor Houbara cells. A total of 302 semen samples from 34 chimeric roosters were analyzed and eight were confirmed as germline chimeras. Semen samples from these eight roosters were used to artificially inseminate three female Houbara bustards. Subsequently, 45 Houbara eggs were obtained and incubated, two of which were fertile. One egg hatched as a male live born Houbara; the other was female but died before hatching. Genotyping confirmed that the male chick was a pure-line Houbara derived from a chimeric rooster.This study demonstrates for the first time that Houbara gPGCs can migrate, differentiate and eventually give rise to functional sperm in the chimeric chicken testis. This approach may provide a promising tool for propagation and conservation of endangered avian

  14. Modulated microwave microscopy and probes used therewith

    Science.gov (United States)

    Lai, Keji; Kelly, Michael; Shen, Zhi-Xun

    2012-09-11

    A microwave microscope including a probe tip electrode vertically positionable over a sample and projecting downwardly from the end of a cantilever. A transmission line connecting the tip electrode to the electronic control system extends along the cantilever and is separated from a ground plane at the bottom of the cantilever by a dielectric layer. The probe tip may be vertically tapped near or at the sample surface at a low frequency and the microwave signal reflected from the tip/sample interaction is demodulated at the low frequency. Alternatively, a low-frequency electrical signal is also a non-linear electrical element associated with the probe tip to non-linearly interact with the applied microwave signal and the reflected non-linear microwave signal is detected at the low frequency. The non-linear element may be semiconductor junction formed near the apex of the probe tip or be an FET formed at the base of a semiconducting tip.

  15. Evaluation of a Novel Temperature Sensing Probe for Monitoring and Controlling Glass Temperature in a Joule-Heated Glass Melter

    International Nuclear Information System (INIS)

    Watkins, A. D.; Musick, C. A.; Cannon, C.; Carlson, N. M.; Mullenix, P.D.; Tillotson, R. D.

    1999-01-01

    A self-verifying temperature sensor that employs advanced contact thermocouple probe technology was tested in a laboratory-scale, joule-heated, refractory-lined glass melter used for radioactive waste vitrification. The novel temperature probe monitors melt temperature at any given level of the melt chamber. The data acquisition system provides the real-time temperature for molten glass. Test results indicate that the self-verifying sensor is more accurate and reliable than classic platinum/rhodium thermocouple and sheath assemblies. The results of this test are reported as well as enhancements being made to the temperature probe. To obtain more reliable temperature measurements of the molten glass for improving production efficiency and ensuring consistent glass properties, optical sensing was reviewed for application in a high temperature environment

  16. Millimeter-wave active probe

    Science.gov (United States)

    Majidi-Ahy, Gholamreza; Bloom, David M.

    1991-01-01

    A millimeter-wave active probe for use in injecting signals with frequencies above 50GHz to millimeter-wave and ultrafast devices and integrated circuits including a substrate upon which a frequency multiplier consisting of filter sections and impedance matching sections are fabricated in uniplanar transmission line format. A coaxial input and uniplanar 50 ohm transmission line couple an approximately 20 GHz input signal to a low pass filter which rolls off at approximately 25 GHz. An input impedance matching section couples the energy from the low pass filter to a pair of matched, antiparallel beam lead diodes. These diodes generate odd-numberd harmonics which are coupled out of the diodes by an output impedance matching network and bandpass filter which suppresses the fundamental and third harmonics and selects the fifth harmonic for presentation at an output.

  17. The Interstellar Ethics of Self-Replicating Probes

    Science.gov (United States)

    Cooper, K.

    Robotic spacecraft have been our primary means of exploring the Universe for over 50 years. Should interstellar travel become reality it seems unlikely that humankind will stop using robotic probes. These probes will be able to replicate themselves ad infinitum by extracting raw materials from the space resources around them and reconfiguring them into replicas of themselves, using technology such as 3D printing. This will create a colonising wave of probes across the Galaxy. However, such probes could have negative as well as positive consequences and it is incumbent upon us to factor self-replicating probes into our interstellar philosophies and to take responsibility for their actions.

  18. Nanolithography and nanochemistry utilizing scanning probe techniques: directed self-assembly of sub-micrometer-sized structures by scanning probe lithography defined templates

    NARCIS (Netherlands)

    Wouters, D.; Sturms, J.P.E.; Schubert, U.S.

    2004-01-01

    The octadecyl trichlorosilane (OTS) monolayer was formed on Si carrier, and the template regulated by a local probe oxidation method from this was produced using a scanning probe lithography. The local probe oxidation was done by moving an AFM tip along an axle line. When the chip contacts a OTS

  19. Gravity Probe B Encapsulated

    Science.gov (United States)

    2004-01-01

    In this photo, the Gravity Probe B (GP-B) space vehicle is being encapsulated atop the Delta II launch vehicle. The GP-B is the relativity experiment developed at Stanford University to test two extraordinary predictions of Albert Einstein's general theory of relativity. The experiment will measure, very precisely, the expected tiny changes in the direction of the spin axes of four gyroscopes contained in an Earth-orbiting satellite at a 400-mile altitude. So free are the gyroscopes from disturbance that they will provide an almost perfect space-time reference system. They will measure how space and time are very slightly warped by the presence of the Earth, and, more profoundly, how the Earth's rotation very slightly drags space-time around with it. These effects, though small for the Earth, have far-reaching implications for the nature of matter and the structure of the Universe. GP-B is among the most thoroughly researched programs ever undertaken by NASA. This is the story of a scientific quest in which physicists and engineers have collaborated closely over many years. Inspired by their quest, they have invented a whole range of technologies that are already enlivening other branches of science and engineering. Launched April 20, 2004 , the GP-B program was managed for NASA by the Marshall Space Flight Center. Development of the GP-B is the responsibility of Stanford University along with major subcontractor Lockheed Martin Corporation. (Image credit to Russ Underwood, Lockheed Martin Corporation).

  20. Investigation of The regularities of the process and development of method of management of technological line operation within the process of mass raw mate-rials supply in terms of dynamics of inbound traffic of unit trains

    Directory of Open Access Journals (Sweden)

    Катерина Ігорівна Сізова

    2015-03-01

    Full Text Available Large-scale sinter plants at metallurgical enterprises incorporate highly productive transport-and-handling complexes (THC that receive and process mass iron-bearing raw materials. Such THCs as a rule include unloading facilities and freight railway station. The central part of the THC is a technological line that carries out operations of reception and unloading of unit trains with raw materials. The technological line consists of transport and freight modules. The latter plays a leading role and, in its turn, consists of rotary car dumpers and conveyor belts. This module represents a determinate system that carries out preparation and unloading operations. Its processing capacity is set in accordance with manufacturing capacity of the sinter plant. The research has shown that in existing operating conditions, which is characterized by “arrhythmia” of interaction between external transport operation and production, technological line of THC functions inefficiently. Thus, it secures just 18-20 % of instances of processing of inbound unit trains within set standard time. It was determined that duration of the cycle of processing of inbound unit train can play a role of regulator, under stochastic characteristics of intervals between inbound unit trains with raw materials on the one hand, and determined unloading system on the other hand. That is why evaluation of interdependence between these factors allows determination of duration of cycle of processing of inbound unit trains. Basing on the results of the study, the method of logistical management of the processing of inbound unit trains was offered. At the same time, real duration of processing of inbound unit train is taken as the regulated value. The regulation process implies regular evaluation and comparison of these values, and, taking into account different disturbances, decision-making concerning adaptation of functioning of technological line. According to the offered principles

  1. Micromachined fiber optic Fabry-Perot underwater acoustic probe

    Science.gov (United States)

    Wang, Fuyin; Shao, Zhengzheng; Hu, Zhengliang; Luo, Hong; Xie, Jiehui; Hu, Yongming

    2014-08-01

    One of the most important branches in the development trend of the traditional fiber optic physical sensor is the miniaturization of sensor structure. Miniature fiber optic sensor can realize point measurement, and then to develop sensor networks to achieve quasi-distributed or distributed sensing as well as line measurement to area monitoring, which will greatly extend the application area of fiber optic sensors. The development of MEMS technology brings a light path to address the problems brought by the procedure of sensor miniaturization. Sensors manufactured by MEMS technology possess the advantages of small volume, light weight, easy fabricated and low cost. In this paper, a fiber optic extrinsic Fabry-Perot interferometric underwater acoustic probe utilizing micromachined diaphragm collaborated with fiber optic technology and MEMS technology has been designed and implemented to actualize underwater acoustic sensing. Diaphragm with central embossment, where the embossment is used to anti-hydrostatic pressure which would largely deflect the diaphragm that induce interferometric fringe fading, has been made by double-sided etching of silicon on insulator. By bonding the acoustic-sensitive diaphragm as well as a cleaved fiber end in ferrule with an outer sleeve, an extrinsic Fabry-Perot interferometer has been constructed. The sensor has been interrogated by quadrature-point control method and tested in field-stable acoustic standing wave tube. Results have been shown that the recovered signal detected by the sensor coincided well with the corresponding transmitted signal and the sensitivity response was flat in frequency range from 10 Hz to 2kHz with the value about -154.6 dB re. 1/μPa. It has been manifest that the designed sensor could be used as an underwater acoustic probe.

  2. RECURSOS TECNOLÓGICOS AUDIOVISUALES DE FORMACIÓN EN RED: SISTEMAS STREAMING MEDIA Y TELEINMERSIVOS TECHNOLOGICAL RESOURCES FOR ON-LINE INSTRUCTION: STREAMING MEDIA AND TELEIMMERSION SYSTEMS

    Directory of Open Access Journals (Sweden)

    Juan Antonio Juanes Méndez

    2010-06-01

    Full Text Available Presentamos dos modalidades tecnológicas de enseñanza en red: la consolidada y ampliamente utilizada tecnología video-streaming, y el futuro de la comunicación a distancia, la teleinmersión. La primera permite la transmisión de audio/vídeo por la red para que puede ser vista por el usuario en su ordenador personal, desde cualquier lugar que disponga de una conexión a red. La información será recibida y decodificada por el usuario final utilizando cualquier reproductor de los que existen en el mercado. La teleinmersión, por su parte, permite crear espacios virtuales de colaboración entre profesionales, ofreciendo entornos muy cercanos a la realidad. Esta tecnología revolucionará, sin duda, nuestros sistemas de enseñanza en los próximos años, facilitando la interacción profesor-alumno. Es evidente que la formación e-learning aporta a los alumnos y a los docentes grandes ventajas como: menores tiempos de aprendizaje, flexibilidad de horarios y de ubicación geográfica, entre otras. We describe two technological modes of on-line teaching: the consolidated and widely used video-streaming mode and teleimmersion, the future of distance communications. The former mode allows the transmission of audio/video through the network so that it can be seen by the user on a PC from anywhere harbouring a network connection. The information is received and decoded by the final user using any reproducer available on the market. Teleimmersion allows the creation of virtual spaces for collaboration among professionals, offering venues that are very similar to reality. This technology will undoubtedly revolutionize our teaching systems in the near future, facilitating instructor-student interaction. It is clear that e-learning- instruction offers both students and instructors huge advantages, such as shorter learning times and schedule and geographic flexibility, among others.

  3. Evaluation of software sensors for on-line estimation of culture conditions in an Escherichia coli cultivation expressing a recombinant protein.

    Science.gov (United States)

    Warth, Benedikt; Rajkai, György; Mandenius, Carl-Fredrik

    2010-05-03

    Software sensors for monitoring and on-line estimation of critical bioprocess variables have mainly been used with standard bioreactor sensors, such as electrodes and gas analyzers, where algorithms in the software model have generated the desired state variables. In this article we propose that other on-line instruments, such as NIR probes and on-line HPLC, should be used to make more reliable and flexible software sensors. Five software sensor architectures were compared and evaluated: (1) biomass concentration from an on-line NIR probe, (2) biomass concentration from titrant addition, (3) specific growth rate from titrant addition, (4) specific growth rate from the NIR probe, and (5) specific substrate uptake rate and by-product rate from on-line HPLC and NIR probe signals. The software sensors were demonstrated on an Escherichia coli cultivation expressing a recombinant protein, green fluorescent protein (GFP), but the results could be extrapolated to other production organisms and product proteins. We conclude that well-maintained on-line instrumentation (hardware sensors) can increase the potential of software sensors. This would also strongly support the intentions with process analytical technology and quality-by-design concepts. 2010 Elsevier B.V. All rights reserved.

  4. Probe tests microweld strength

    Science.gov (United States)

    1965-01-01

    Probe is developed to test strength of soldered, brazed or microwelded joints. It consists of a spring which may be adjusted to the desired test pressure by means of a threaded probe head, and an indicator lamp. Device may be used for electronic equipment testing.

  5. Use of moisture probes in building materials industry

    International Nuclear Information System (INIS)

    Hanke, L.

    A neutron probe to be built in the production line was developed for monitoring moisture content of bulk materials and suspensions of all types in the building material industry. The probe is dust- and external moisture-protected. The probe measuring capacity is about 100 l, the mean measurement error is +- 0.008 g water per 1 cm 3 , which for fine sand represents an error of +- 0.3%. The probe is connected via a cable to a measuring instrument showing an electrical value proportional to the measured material moisture content. (Z.M.)

  6. Tools to probe the universe

    International Nuclear Information System (INIS)

    Lagage, P.O.; Augueres, J.L.; Amiaux, J.; Cara, Ch.; Fontignie, J.; Rio, Y.; Fermon, C.; Pannetier-Lecoeur, M.; De Vismes, A.; Cordier, B.; Fesquet, M.; Ferrando, Ph.; Authier, M.; Pantin, E.; Glicenstein, J.F.; Boulade, O.; Refregier, A.; Stolarczyk, Th.; Agnese, P.; Rodriguez, L.; Agnese, P.; Pigot, C.; Duband, L.; Limousin, O.; Delagnes, E.; Turck-Chieze, S.; Carton, P.H.; Starck, J.L.; Bournaud, F.; Teyssier, R.; Audit, E.; Brun, A.S.; Leca, P.; Menache, Ch.; Pomarede, D.; Thooris, B.; Meis, C.

    2009-01-01

    This special issue of Clefs CEA journal is entirely devoted to astrophysics and to the exploration and probing of the Universe. The second part of this dossier, described here, makes a status of the tools used to probe the universe: telescopes, imaging spectrometers, data processing and simulation. Content: A - Telescopes of the future: 1. Seeing further out: JWST: looking back on a past 13 billion years old, Space specifics: the learning curve to know-how, Fabricating a corona-graph mask, SVOM, a satellite to detect the explosions of the first stars to be formed in the Universe; 2. Seeing more precisely: SIMBOL-X, pioneering formation flying, ELT/METIS, a 42-meter giant, One hundred telescopes for the CTA arrays; 3. Seeing wider: Euclid, mapping the extragalactic sky, ANTARES: the neutrino, another cosmic messenger; B - The new generation of imaging spectrometers: Observing the Universe in the submillimeter spectral region, The X-ray Universe, Space cryo-coolers, Out in the extreme, tumultuous Universe, Probing the Sun with GOLF-NG, Focus: From light to imagery; C - Data analysis in astrophysics; D - Numerical simulation in astrophysics: Information technology and theoretical predictions in astrophysics, Supercomputers for a better understanding of the Universe, The visualization of astrophysical simulations, Godunov, a numerical platform for education and research

  7. Isotropic Broadband E-Field Probe

    Directory of Open Access Journals (Sweden)

    Béla Szentpáli

    2008-01-01

    Full Text Available An E-field probe has been developed for EMC immunity tests performed in closed space. The leads are flexible resistive transmission lines. Their influence on the field distribution is negligible. The probe has an isotropic reception from 100 MHz to 18 GHz; the sensitivity is in the 3 V/m–10 V/m range. The device is an accessory of the EMC test chamber. The readout of the field magnitude is carried out by personal computer, which fulfils also the required corrections of the raw data.

  8. Report about the contribution of new technologies to the burial of high and very-high voltage power lines; Rapport sur l'apport de nouvelles technologies dans l'enfouissement des lignes electriques a haute et tres haute tension

    Energy Technology Data Exchange (ETDEWEB)

    Kert, Ch. [Assemblee Nationale, 75 - Paris (France)

    2001-12-01

    Today, high voltage overhead power lines are badly perceived by the population in particular in urban areas and in areas with remarkable landscapes, for their visual impact on the environment and for their possible effect on human health. With the increase of the domestic and foreign power demand, the high voltage grid will develop in the future and the partial burial of power lines can be the first steps towards a solution to these problems. This report makes a worldwide status of the state-of-the-art of power line burial technologies, all voltages considered, and then focusses on the very-high voltage challenge which encounters the most important technological and economical constraints. The technical feasibility has been proven but the lowering of costs needs the development of a real European market of power lines burial. (J.S.)

  9. Gravity Probe B Assembled

    Science.gov (United States)

    2000-01-01

    In this photo, the Gravity Probe B (GP-B) space vehicle is being assembled at the Sunnyvale, California location of the Lockheed Martin Corporation. The GP-B is the relativity experiment developed at Stanford University to test two extraordinary predictions of Albert Einstein's general theory of relativity. The experiment will measure, very precisely, the expected tiny changes in the direction of the spin axes of four gyroscopes contained in an Earth-orbiting satellite at a 400-mile altitude. So free are the gyroscopes from disturbance that they will provide an almost perfect space-time reference system. They will measure how space and time are very slightly warped by the presence of the Earth, and, more profoundly, how the Earth's rotation very slightly drags space-time around with it. These effects, though small for the Earth, have far-reaching implications for the nature of matter and the structure of the Universe. GP-B is among the most thoroughly researched programs ever undertaken by NASA. This is the story of a scientific quest in which physicists and engineers have collaborated closely over many years. Inspired by their quest, they have invented a whole range of technologies that are already enlivening other branches of science and engineering. Launched April 20, 2004 , the GP-B program was managed for NASA by the Marshall Space Flight Center. Development of the GP-B is the responsibility of Stanford University along with major subcontractor Lockheed Martin Corporation. (Image credit to Russ Underwood, Lockheed Martin Corporation).

  10. Analytical expressions for correction factors for noise measurements with a four-point probe

    NARCIS (Netherlands)

    Vandamme, L.K.J.; Leroy, G.

    2006-01-01

    The linear four-point probe method is useful to measure the resistivity, by passing a current I14 through the outer probes and by measuring the voltage V23 between the inner probes. The contacts are on a line and denoted by 1, 2, 3, 4, respectively. The sheet resistance for thin layers with

  11. Study of Wireless Transmission Protocol Technology for Use in Flight Line Environment to Assist the Data Loading and Downloading on Aircraft

    National Research Council Canada - National Science Library

    Meng, Ow

    2004-01-01

    ... into the use of wireless tmnsmission technology to complement or replace the manual method of loading the critical data file from the command station onto every F-16 aircraft, The present wireless technology...

  12. Field emission sensing for non-contact probe recording

    NARCIS (Netherlands)

    le Fèbre, A.J.

    2008-01-01

    In probe recording an array of thousands of nanometer-sharp probes is used to write and read on a storage medium. By using micro-electromechanical system technology (MEMS) for fabrication, small form factor memories with high data density and low power consumption can be obtained. Such a system is

  13. A Hall probe technique for characterizing high-temperature superconductors

    International Nuclear Information System (INIS)

    Zhang, J.; Sheldon, P.; Ahrenkiel, R.K.

    1992-01-01

    Thin-film GaAs Hall probes were fabricated by molecular beam epitaxy technology. A contactless technique was developed to characterize thin-film, high-temperature superconducting (HTSC) materials. The Hall probes detected the ac magnetic flux penetration through the high-temperature superconducting materials. The Hall detector has advantages over the mutual inductance magnetic flux detector

  14. SYTO probes: markers of apoptotic cell demise.

    Science.gov (United States)

    Wlodkowic, Donald; Skommer, Joanna

    2007-10-01

    As mechanistic studies on tumor cell death advance towards their ultimate translational goal, there is a need for specific, rapid, and high-throughput analytical tools to detect diverse cell demise modes. Patented DNA-binding SYTO probes, for example, are gaining increasing interest as easy-to-use markers of caspase-dependent apoptotic cell death. They are proving convenient for tracking apoptosis in diverse hematopoietic cell lines and primary tumor samples, and, due to their spectral characteristics, appear to be useful for the development of multiparameter flow cytometry assays. Herein, several protocols for multiparametric assessment of apoptotic events using SYTO probes are provided. There are protocols describing the use of green fluorescent SYTO 16 and red fluorescent SYTO 17 dyes in combination with plasma membrane permeability markers. Another protocol highlights the multiparametric use of SYTO 16 dye in conjunction with the mitochondrial membrane potential sensitive probe, tetramethylrhodamine methyl ester (TMRM), and the plasma membrane permeability marker, 7-aminoactinomycin D (7-AAD).

  15. Hard probes 2006 Asilomar

    CERN Multimedia

    2006-01-01

    "The second international conference on hard and electromagnetic probes of high-energy nuclear collisions was held June 9 to 16, 2006 at the Asilomar Conference grounds in Pacific Grove, California" (photo and 1/2 page)

  16. Neutrons as a probe

    International Nuclear Information System (INIS)

    Iizumi, Masashi

    1993-01-01

    As an introduction to the symposium a brief overview will be given about the features of neutrons as a probe. First it will be pointed out that the utilization of neutrons as a probe for investigating the structural and dynamical properties of condensed matters is a benign gift eventuated from the release of atomic energy initiated by Enrico Fermi exactly half century ago. Features of neutrons as a probe are discussed in accordance with the four basic physical properties of neutrons as an elementary particle; (1) no electric charge (the interaction with matter is nuclear), (2) the mass of neutron is 1 amu, (3) spin is 1/2 and (4) neutrons have magnetic dipole moment. Overview will be given on the uniqueness of neutrons as a probe and on the variety in the way they are used in the wide research area from the pure science to the industrial applications. (author)

  17. First human hNT neurons patterned on parylene-C/silicon dioxide substrates: Combining an accessible cell line and robust patterning technology for the study of the pathological adult human brain.

    Science.gov (United States)

    Unsworth, C P; Graham, E S; Delivopoulos, E; Dragunow, M; Murray, A F

    2010-12-15

    In this communication, we describe a new method which has enabled the first patterning of human neurons (derived from the human teratocarcinoma cell line (hNT)) on parylene-C/silicon dioxide substrates. We reveal the details of the nanofabrication processes, cell differentiation and culturing protocols necessary to successfully pattern hNT neurons which are each key aspects of this new method. The benefits in patterning human neurons on silicon chip using an accessible cell line and robust patterning technology are of widespread value. Thus, using a combined technology such as this will facilitate the detailed study of the pathological human brain at both the single cell and network level. Copyright © 2010 Elsevier B.V. All rights reserved.

  18. Multi-point probe for testing electrical properties and a method of producing a multi-point probe

    DEFF Research Database (Denmark)

    2011-01-01

    A multi-point probe for testing electrical properties of a number of specific locations of a test sample comprises a supporting body defining a first surface, a first multitude of conductive probe arms (101-101'''), each of the probe arms defining a proximal end and a distal end. The probe arms...... of contact with the supporting body, and a maximum thickness perpendicular to its perpendicular bisector and its line of contact with the supporting body. Each of the probe arms has a specific area or point of contact (111-111''') at its distal end for contacting a specific location among the number...... of specific locations of the test sample. At least one of the probe arms has an extension defining a pointing distal end providing its specific area or point of contact located offset relative to its perpendicular bisector....

  19. Adjustable Pitot Probe

    Science.gov (United States)

    Ashby, George C., Jr.; Robbins, W. Eugene; Horsley, Lewis A.

    1991-01-01

    Probe readily positionable in core of uniform flow in hypersonic wind tunnel. Formed of pair of mating cylindrical housings: transducer housing and pitot-tube housing. Pitot tube supported by adjustable wedge fairing attached to top of pitot-tube housing with semicircular foot. Probe adjusted both radially and circumferentially. In addition, pressure-sensing transducer cooled internally by water or other cooling fluid passing through annulus of cooling system.

  20. Building versatile bipartite probes for quantum metrology

    Science.gov (United States)

    Farace, Alessandro; De Pasquale, Antonella; Adesso, Gerardo; Giovannetti, Vittorio

    2016-01-01

    We consider bipartite systems as versatile probes for the estimation of transformations acting locally on one of the subsystems. We investigate what resources are required for the probes to offer a guaranteed level of metrological performance, when the latter is averaged over specific sets of local transformations. We quantify such a performance via the average skew information (AvSk), a convex quantity which we compute in closed form for bipartite states of arbitrary dimensions, and which is shown to be strongly dependent on the degree of local purity of the probes. Our analysis contrasts and complements the recent series of studies focused on the minimum, rather than the average, performance of bipartite probes in local estimation tasks, which was instead determined by quantum correlations other than entanglement. We provide explicit prescriptions to characterize the most reliable states maximizing the AvSk, and elucidate the role of state purity, separability and correlations in the classification of optimal probes. Our results can help in the identification of useful resources for sensing, estimation and discrimination applications when complete knowledge of the interaction mechanism realizing the local transformation is unavailable, and access to pure entangled probes is technologically limited.

  1. Building versatile bipartite probes for quantum metrology

    International Nuclear Information System (INIS)

    Farace, Alessandro; Pasquale, Antonella De; Giovannetti, Vittorio; Adesso, Gerardo

    2016-01-01

    We consider bipartite systems as versatile probes for the estimation of transformations acting locally on one of the subsystems. We investigate what resources are required for the probes to offer a guaranteed level of metrological performance, when the latter is averaged over specific sets of local transformations. We quantify such a performance via the average skew information (AvSk), a convex quantity which we compute in closed form for bipartite states of arbitrary dimensions, and which is shown to be strongly dependent on the degree of local purity of the probes. Our analysis contrasts and complements the recent series of studies focused on the minimum, rather than the average, performance of bipartite probes in local estimation tasks, which was instead determined by quantum correlations other than entanglement. We provide explicit prescriptions to characterize the most reliable states maximizing the AvSk, and elucidate the role of state purity, separability and correlations in the classification of optimal probes. Our results can help in the identification of useful resources for sensing, estimation and discrimination applications when complete knowledge of the interaction mechanism realizing the local transformation is unavailable, and access to pure entangled probes is technologically limited. (paper)

  2. Reports in the area sensor technology: Part 2: Dynamic deviations which may appear via the sensing lines in measurement of reactor pressure and level

    International Nuclear Information System (INIS)

    Bergdahl, Bengt-Goeran

    2002-12-01

    The sensors are part of the safety system in a nuclear power plant. They are the first link in a chain of components, which affect the safety system. It is therefore of great importance that the sensors fulfil tough requirements on reliability and response time. In practice, the dynamic qualities of the sensors in a BWR are seldom, or never, tested. The static qualities, on the other hand, are controlled every year when the transmitters are calibrated. This is done during the regular outage of the power plant. It is common that several sensors are connected to the same sensing line. This is true especially in old reactors, where only a few pressure taps are available in the reactor pressure vessel. This is a troublesome disadvantage in the design, since a failure in one sensing line will affect all components, which are connected to that sensing line ('Common Cause Failure'). This report focuses on deviations in the measurement system connected to the sensing lines. The deviations are illustrated by examples from Swedish and foreign BWRs. The sensing lines are mechanically passive components. They can reduce a system's response time even if there are now deviations in the static presentation. This report mentions cases in nuclear power plants, where the time constant of the sensing line has changed from 0.1 seconds, which is a normal response time, to 5 seconds. This has happened because of a gradual blockage of the sensing line. Today, signal analysis is the only way to examine the dynamic qualities of sensing lines. Filtrations can be unveiled by measuring and analysing the transmitter signal, regardless the location of the filtration: in the sensing line, in the transmitter or in other instrumentation components. A practical case is presented where pulsation dampers with so-called needles were used at Ringhals 1 in Sweden. Their influence on the response time for the measurement signal corresponds to a time constant = 0.55 s. By eliminating the needles the

  3. Generation of a human induced pluripotent stem cell line from urinary cells of a patient with primary congenital glaucoma using integration free Sendai technology.

    Science.gov (United States)

    Zhang, Jingxue; Wu, Shen; Hu, Man; Liu, Qian

    2018-04-09

    We have generated a human induced pluripotent stem cell (iPSC) line derived from urinary cells of a 10years old patient with primary congenital glaucoma (PCG). The cells were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system and shown to have full differentiation potential. The line is available and registered in the human pluripotent stem cell registry as BIOi001-A. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

  4. LOFAR and APERTIF Surveys of the Radio Sky: Probing Shocks ...

    Indian Academy of Sciences (India)

    LOFAR and APERTIF Surveys of the Radio Sky: Probing Shocks and Magnetic .... technology. This replaces the traditional and expensive mechanical dishes by a com- ... approach has been adopted (for details, see Röttgering et al. 2010).

  5. Micro- and nanodevices integrated with biomolecular probes.

    Science.gov (United States)

    Alapan, Yunus; Icoz, Kutay; Gurkan, Umut A

    2015-12-01

    Understanding how biomolecules, proteins and cells interact with their surroundings and other biological entities has become the fundamental design criterion for most biomedical micro- and nanodevices. Advances in biology, medicine, and nanofabrication technologies complement each other and allow us to engineer new tools based on biomolecules utilized as probes. Engineered micro/nanosystems and biomolecules in nature have remarkably robust compatibility in terms of function, size, and physical properties. This article presents the state of the art in micro- and nanoscale devices designed and fabricated with biomolecular probes as their vital constituents. General design and fabrication concepts are presented and three major platform technologies are highlighted: microcantilevers, micro/nanopillars, and microfluidics. Overview of each technology, typical fabrication details, and application areas are presented by emphasizing significant achievements, current challenges, and future opportunities. Copyright © 2015 Elsevier Inc. All rights reserved.

  6. Model for resonant plasma probe.

    Energy Technology Data Exchange (ETDEWEB)

    Warne, Larry Kevin; Johnson, William Arthur; Hebner, Gregory Albert; Jorgenson, Roy E.; Coats, Rebecca Sue

    2007-04-01

    This report constructs simple circuit models for a hairpin shaped resonant plasma probe. Effects of the plasma sheath region surrounding the wires making up the probe are determined. Electromagnetic simulations of the probe are compared to the circuit model results. The perturbing effects of the disc cavity in which the probe operates are also found.

  7. High Line

    DEFF Research Database (Denmark)

    Kiib, Hans

    2015-01-01

    At just over 10 meters above street level, the High Line extends three kilometers through three districts of Southwestern Manhattan in New York. It consists of simple steel construction, and previously served as an elevated rail line connection between Penn Station on 34th Street and the many....... The High Line project has been carried out as part of an open conversion strategy. The result is a remarkable urban architectural project, which works as a catalyst for the urban development of Western Manhattan. The greater project includes the restoration and reuse of many old industrial buildings...

  8. Convective heat flow probe

    Science.gov (United States)

    Dunn, James C.; Hardee, Harry C.; Striker, Richard P.

    1985-01-01

    A convective heat flow probe device is provided which measures heat flow and fluid flow magnitude in the formation surrounding a borehole. The probe comprises an elongate housing adapted to be lowered down into the borehole; a plurality of heaters extending along the probe for heating the formation surrounding the borehole; a plurality of temperature sensors arranged around the periphery of the probe for measuring the temperature of the surrounding formation after heating thereof by the heater elements. The temperature sensors and heater elements are mounted in a plurality of separate heater pads which are supported by the housing and which are adapted to be radially expanded into firm engagement with the walls of the borehole. The heat supplied by the heater elements and the temperatures measured by the temperature sensors are monitored and used in providing the desired measurements. The outer peripheral surfaces of the heater pads are configured as segments of a cylinder and form a full cylinder when taken together. A plurality of temperature sensors are located on each pad so as to extend along the length and across the width thereof, with a heating element being located in each pad beneath the temperature sensors. An expansion mechanism driven by a clamping motor provides expansion and retraction of the heater pads and expandable packer-type seals are provided along the probe above and below the heater pads.

  9. Theory of NMR probe design

    International Nuclear Information System (INIS)

    Schnall, M.D.

    1988-01-01

    The NMR probe is the intrinsic part of the NMR system which allows transmission of a stimulus to a sample and the reception of a resulting signal from a sample. NMR probes are used in both imaging and spectroscopy. Optimal probe design is important to the production of adequate signal/moise. It is important for anyone using NMR techniques to understand how NMR probes work and how to optimize probe design

  10. Probing into the Secret of the Chinese Air Force.

    Science.gov (United States)

    1983-11-30

    Ri35 968 PROBING INTO THE SECRET OF THE CHINESE AIR FOREE(IJ 1/2 FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON RFB OH 9 38 NOV 83 FTD-ID(,RS)T 1088 3...FOREIGN TECHNOLOGY DIVISION. PROBING INTO THE SECRET OF THE CHINESE AIRFORCE CL1 Approved for public re.lease; distribution unlimited C=)X ~ EET...MICROFICHE NR: FTD-83-C-001469 PROBING INTO THE SECRET OF THE CHINESE AIRFORCE -" -English pages: 111 Source: Enclosure to IR 6 842 0088 83-Booklet

  11. World lines.

    OpenAIRE

    Waser Jürgen; Fuchs Raphael; Ribicic Hrvoje; Schindler Benjamin; Blöschl Günther; Gröller Eduard

    2010-01-01

    In this paper we present World Lines as a novel interactive visualization that provides complete control over multiple heterogeneous simulation runs. In many application areas decisions can only be made by exploring alternative scenarios. The goal of the suggested approach is to support users in this decision making process. In this setting the data domain is extended to a set of alternative worlds where only one outcome will actually happen. World Lines integrate simulation visualization and...

  12. Single-cell intracellular nano-pH probes.

    Science.gov (United States)

    Özel, Rıfat Emrah; Lohith, Akshar; Mak, Wai Han; Pourmand, Nader

    2015-01-01

    Within a large clonal population, such as cancerous tumor entities, cells are not identical, and the differences between intracellular pH levels of individual cells may be important indicators of heterogeneity that could be relevant in clinical practice, especially in personalized medicine. Therefore, the detection of the intracellular pH at the single-cell level is of great importance to identify and study outlier cells. However, quantitative and real-time measurements of the intracellular pH of individual cells within a cell population is challenging with existing technologies, and there is a need to engineer new methodologies. In this paper, we discuss the use of nanopipette technology to overcome the limitations of intracellular pH measurements at the single-cell level. We have developed a nano-pH probe through physisorption of chitosan onto hydroxylated quartz nanopipettes with extremely small pore sizes (~100 nm). The dynamic pH range of the nano-pH probe was from 2.6 to 10.7 with a sensitivity of 0.09 units. We have performed single-cell intracellular pH measurements using non-cancerous and cancerous cell lines, including human fibroblasts, HeLa, MDA-MB-231 and MCF-7, with the pH nanoprobe. We have further demonstrated the real-time continuous single-cell pH measurement capability of the sensor, showing the cellular pH response to pharmaceutical manipulations. These findings suggest that the chitosan-functionalized nanopore is a powerful nano-tool for pH sensing at the single-cell level with high temporal and spatial resolution.

  13. Plutonium helps probe protein, superconductor

    International Nuclear Information System (INIS)

    Anon.

    1990-01-01

    Scientists are finding that plutonium can be a useful research tool that may help them answer important questions in fields as diverse as biochemistry and solid-state physics. This paper reports that U.S. research involving plutonium is confined to the Department of Energy's national laboratories and centers around nuclear weapons technology, waste cleanup and disposal, and health effects. But at Los Alamos National Laboratory, scientists also are using plutonium to probe the biochemical behavior of calmodulin, a key calcium-binding protein that mediates calcium-regulated processes in biological systems. At Argonne National Laboratory, another team is trying to learn how a superconductor's properties are affected by the 5f electrons of an actinide like plutonium

  14. Outsourced Probe Data Effectiveness on Signalized Arterials

    Energy Technology Data Exchange (ETDEWEB)

    Young, Stanley E [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Sharifi, Elham [University of Maryland; Eshragh, Sepideh [University of Maryland; Hamedi, Masoud [University of Maryland; Juster, Reuben M. [University of Maryland; Kaushik, Kartik [University of Maryland

    2017-07-31

    This paper presents results of an I-95 Corridor Coalition sponsored project to assess the ability of outsourced vehicle probe data to provide accurate travel time on signalized roadways for the purposes of real-time operations as well as performance measures. The quality of outsourced probe data on freeways has led many departments of transportation to consider such data for arterial performance monitoring. From April 2013 through June of 2014, the University of Maryland Center for Advanced Transportation Technology gathered travel times from several arterial corridors within the mid-Atlantic region using Bluetooth traffic monitoring (BTM) equipment, and compared these travel times with the data reported to the I95 Vehicle Probe Project (VPP) from an outsourced probe data vendor. The analysis consisted of several methodologies: (1) a traditional analysis that used precision and bias speed metrics; (2) a slowdown analysis that quantified the percentage of significant traffic disruptions accurately captured in the VPP data; (3) a sampled distribution method that uses overlay methods to enhance and analyze recurring congestion patterns. (4) Last, the BTM and VPP data from each 24-hour period of data collection were reviewed by the research team to assess the extent to which VPP captured the nature of the traffic flow. Based on the analysis, probe data is recommended only on arterial roadways with signal densities (measured in signals per mile) up to one, and it should be tested and used with caution for signal densities between one and two, and is not recommended when signal density exceeds two.

  15. Technology Innovation

    Science.gov (United States)

    EPA produces innovative technologies and facilitates their creation in line with the Agency mission to create products such as the stormwater calculator, remote sensing, innovation clusters, and low-cost air sensors.

  16. Structure of the drip line nuclei probed by separation energies

    Czech Academy of Sciences Publication Activity Database

    Dlouhý, Zdeněk

    2006-01-01

    Roč. 15, č. 7 (2006), s. 1471-1475 ISSN 0218-3013 Institutional research plan: CEZ:AV0Z10480505 Keywords : Neutron-rich nuclei * Shell- modell * Magic-number Subject RIV: BE - Theoretical Physics Impact factor: 0.810, year: 2006

  17. Utility of Line Probe Assay for diagnosis of extrapulmonary tuberculosis

    Directory of Open Access Journals (Sweden)

    Joveria Farooqi

    2015-01-01

    Conclusion: The study shows that LiPA has good overall sensitivity and specificity compared with culture. Although the number of samples was very small, the applicability appears to be most useful in urine and pus specimens and should be explored further as a diagnostic tool in these cases.

  18. Proton decay: spectroscopic probe beyond the proton drip line

    International Nuclear Information System (INIS)

    Seweryniak, D; Davids, C N; Robinson, A; Woods, P J; Blank, B; Carpenter, M P; Davinson, T; Freeman, S J; Hammond, N; Hoteling, N; Janssens, R V F; Khoo, T L; Liu, Z; Mukherjee, G; Shergur, J; Sinha, S; Sonzogni, A A; Walters, W B; Woehr, A

    2005-01-01

    Proton decay has been transformed in recent years from an exotic phenomenon into a powerful spectroscopic tool. The frontiers of experimental and theoretical proton-decay studies will be reviewed. Different aspects of proton decay will be illustrated with recent results on the deformed proton emitter 135 Tb, the odd-odd deformed proton emitter 130 Eu, the complex fine structure in the odd-odd 146 Tm nucleus and on excited states in the transitional proton emitter 145 Tm

  19. One-Probe Search

    DEFF Research Database (Denmark)

    Östlin, Anna; Pagh, Rasmus

    2002-01-01

    We consider dictionaries that perform lookups by probing a single word of memory, knowing only the size of the data structure. We describe a randomized dictionary where a lookup returns the correct answer with probability 1 - e, and otherwise returns don't know. The lookup procedure uses an expan...

  20. Probing the Solar Interior

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 3; Issue 3. Probing the Solar Interior Hearing the Heartbeats of the Sun. Ashok Ambastha. General ... Author Affiliations. Ashok Ambastha1. Joint In-Charge Udaipur Solar Observatory Physical Research laboratory P.O. Box No. 198 Udaipur 313 001, India ...

  1. Flexible position probe assembly

    International Nuclear Information System (INIS)

    Schmitz, J.J.

    1977-01-01

    The combination of a plurality of tubular transducer sections and a flexible supporting member extending through the tubular transducer sections forms a flexible elongated probe of a design suitable for monitoring the level of an element, such as a nuclear magnetically permeable control rod or liquid. 3 claims, 23 figures

  2. A Characterization of 2-Tree Probe Interval Graphs

    Directory of Open Access Journals (Sweden)

    Brown David E.

    2014-08-01

    Full Text Available A graph is a probe interval graph if its vertices correspond to some set of intervals of the real line and can be partitioned into sets P and N so that vertices are adjacent if and only if their corresponding intervals intersect and at least one belongs to P. We characterize the 2-trees which are probe interval graphs and extend a list of forbidden induced subgraphs for such graphs created by Pržulj and Corneil in [2-tree probe interval graphs have a large obstruction set, Discrete Appl. Math. 150 (2005 216-231

  3. MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2a

    Directory of Open Access Journals (Sweden)

    Siegrist Fredy

    2009-01-01

    Full Text Available Abstract MicroRNAs are positive and negative regulators of eukaryotic gene expression that modulate transcript abundance by specific binding to sequence motifs located prevalently in the 3' untranslated regions of target messenger RNAs (mRNA. Interferon-alpha-2a (IFNα induces a large set of protein coding genes mediating antiproliferative and antiviral responses. Here we use a global microarray-based microRNA detection platform to identify genes that are induced by IFNα in hepatoma- or melanoma-derived human tumor cell lines. Despite the enormous differences in expression levels between these models, we were able to identify microRNAs that are upregulated by IFNα in both lines suggesting the possibility that interferon-regulated microRNAs are involved in the transcriptional repression of mRNA relevant to cytokine responses.

  4. Silver linings.

    Science.gov (United States)

    Bultas, Margaret W; Pohlman, Shawn

    2014-01-01

    The purpose of this interpretive phenomenological study was to gain a better understanding of the experiences of 11 mothers of preschool children with autism spectrum disorder (ASD). Mothers were interviewed three times over a 6 week period. Interviews were analyzed using interpretive methods. This manuscript highlights one particular theme-a positive perspective mothers described as the "silver lining." This "silver lining" represents optimism despite the adversities associated with parenting a child with ASD. A deeper understanding of this side of mothering children with ASD may help health care providers improve rapport, communication, and result in more authentic family centered care. Copyright © 2014 Elsevier Inc. All rights reserved.

  5. At-line validation of a process analytical technology approach for quality control of melamine-urea-formaldehyde resin in composite wood-panel production using near infrared spectroscopy.

    Science.gov (United States)

    Meder, Roger; Stahl, Wolfgang; Warburton, Paul; Woolley, Sam; Earnshaw, Scott; Haselhofer, Klaus; van Langenberg, Ken; Ebdon, Nick; Mulder, Roger

    2017-01-01

    The reactivity of melamine-urea-formaldehyde resins is of key importance in the manufacture of engineered wood products such as medium density fibreboard (MDF) and other wood composite products. Often the MDF manufacturing plant has little available information on the resin reactivity other than details of the resin specification at the time of batch manufacture, which often occurs off-site at a third-party resin plant. Often too, fresh resin on delivery at the MDF plant is mixed with variable volume of aged resin in storage tanks, thereby rendering any specification of the fresh resin batch obsolete. It is therefore highly desirable to develop a real-time, at-line or on-line, process analytical technology to monitor the quality of the resin prior to MDF panel manufacture. Near infrared (NIR) spectroscopy has been calibrated against standard quality methods and against 13 C nuclear magnetic resonance (NMR) measures of molecular composition in order to provide at-line process analytical technology (PAT), to monitor the resin quality, particularly the formaldehyde content of the resin. At-line determination of formaldehyde content in the resin was made possible using a six-factor calibration with an R 2 (cal) value of 0.973, and R 2 (CV) value of 0.929 and a root-mean-square error of cross-validation of 0.01. This calibration was then used to generate control charts of formaldehyde content at regular four-hourly periods during MDF panel manufacture in a commercial MDF manufacturing plant.

  6. Recent Advances in Target Characterization and Identification by Photoaffinity Probes

    Directory of Open Access Journals (Sweden)

    Sang J. Chung

    2013-08-01

    Full Text Available Target identification of biologically active molecules such as natural products, synthetic small molecules, peptides, and oligonucleotides mainly relies on affinity chromatography, activity-based probes, or photoaffinity labeling (PAL. Amongst them, activity-based probes and PAL have offered great advantages in target identification technology due to their ability to form covalent bonds with the corresponding targets. Activity-based probe technology mainly relies on the chemical reactivity of the target proteins, thereby limiting the majority of the biological targets to enzymes or proteins which display reactive residues at the probe-binding site. In general, the probes should bear a reactive moiety such as an epoxide, a Michael acceptor, or a reactive alkyl halide in their structures. On the other hand, photoaffinity probes (PAPs are composed of a target-specific ligand and a photoactivatable functional group. When bound to the corresponding target proteins and activated with wavelength-specific light, PAPs generate highly reactive chemical species that covalently cross-link proximal amino acid residues. This process is better known as PAL and is widely employed to identify cellular targets of biologically active molecules. This review highlights recent advances in target identification by PAL, with a focus on the structure and chemistry of the photoaffinity probes developed in the recent decade, coupled to the target proteins identified using these probes.

  7. A New Generation of Thermal Desorption Technology Incorporating Multi Mode Sampling (NRT/DAAMS/Liquid Agent) for Both on and off Line Analysis of Trace Level Airbone Chemical Warfare Agents

    International Nuclear Information System (INIS)

    Roberts, G. M.

    2007-01-01

    A multi functional, twin-trap, electrically-cooled thermal desorption (TD) system (TT24-7) will be discussed for the analysis of airborne trace level chemical warfare agents. This technology can operate in both military environments (CW stockpile, or destruction facilities) and civilian locations where it is used to monitor for accidental or terrorist release of acutely toxic substances. The TD system interfaces to GC, GCMS or direct MS analytical platforms and provides for on-line continuous air monitoring with no sampling time blind spots and within a near real time (NRT) context. Using this technology enables on-line sub ppt levels of agent detection from a vapour sample. In addition to continuous sampling the system has the capacity for off-line single (DAAMS) tube analysis and the ability to receive an external liquid agent injection. The multi mode sampling functionality provides considerable flexibility to the TD system, allowing continuous monitoring of an environment for toxic substances plus the ability to analyse calibration standards. A calibration solution can be introduced via a conventional sampling tube on to either cold trap or as a direct liquid injection using a conventional capillary split/splitless injection port within a gas chromatograph. Low level (linearity) data will be supplied showing the TT24-7 analyzing a variety of CW compounds including free (underivitised) VX using the three sampling modes described above. Stepwise changes in vapor generated agent concentrations will be shown, and this is cross referenced against direct liquid agent introduction, and the tube sampling modes. This technology is in use today in several geographies around the world in both static and mobile analytical laboratories. (author)

  8. Optical Probes for Neurobiological Sensing and Imaging.

    Science.gov (United States)

    Kim, Eric H; Chin, Gregory; Rong, Guoxin; Poskanzer, Kira E; Clark, Heather A

    2018-04-13

    Fluorescent nanosensors and molecular probes are next-generation tools for imaging chemical signaling inside and between cells. Electrophysiology has long been considered the gold standard in elucidating neural dynamics with high temporal resolution and precision, particularly on the single-cell level. However, electrode-based techniques face challenges in illuminating the specific chemicals involved in neural cell activation with adequate spatial information. Measuring chemical dynamics is of fundamental importance to better understand synergistic interactions between neurons as well as interactions between neurons and non-neuronal cells. Over the past decade, significant technological advances in optical probes and imaging methods have enabled entirely new possibilities for studying neural cells and circuits at the chemical level. These optical imaging modalities have shown promise for combining chemical, temporal, and spatial information. This potential makes them ideal candidates to unravel the complex neural interactions at multiple scales in the brain, which could be complemented by traditional electrophysiological methods to obtain a full spatiotemporal picture of neurochemical dynamics. Despite the potential, only a handful of probe candidates have been utilized to provide detailed chemical information in the brain. To date, most live imaging and chemical mapping studies rely on fluorescent molecular indicators to report intracellular calcium (Ca 2+ ) dynamics, which correlates with neuronal activity. Methodological advances for monitoring a full array of chemicals in the brain with improved spatial, temporal, and chemical resolution will thus enable mapping of neurochemical circuits with finer precision. On the basis of numerous studies in this exciting field, we review the current efforts to develop and apply a palette of optical probes and nanosensors for chemical sensing in the brain. There is a strong impetus to further develop technologies capable of

  9. Electronic system for Langmuir probe measurements

    Czech Academy of Sciences Publication Activity Database

    Mitov, M.; Bankova, A.; Dimitrova, M.; Ivanova, P.; Tutulkov, K.; Djermanova, N.; Dejarnac, Renaud; Stöckel, Jan; Popov, Tsv.K.

    2012-01-01

    Roč. 356, č. 1 (2012), s. 012008 ISSN 1742-6588. [InternationalSummerSchoolonVacuum,Electron, and IonTechnologies(VEIT2011)/17./. Sunny Beach, 19.09.2011-23.09.2011] Institutional research plan: CEZ:AV0Z20430508 Keywords : Plasma * tokamak * diagnostics * electric probe Subject RIV: BL - Plasma and Gas Discharge Physics http://iopscience.iop.org/1742-6596/356/1/012008/pdf/1742-6596_356_1_012008.pdf

  10. Nano Mechanical Machining Using AFM Probe

    Science.gov (United States)

    Mostofa, Md. Golam

    Complex miniaturized components with high form accuracy will play key roles in the future development of many products, as they provide portability, disposability, lower material consumption in production, low power consumption during operation, lower sample requirements for testing, and higher heat transfer due to their very high surface-to-volume ratio. Given the high market demand for such micro and nano featured components, different manufacturing methods have been developed for their fabrication. Some of the common technologies in micro/nano fabrication are photolithography, electron beam lithography, X-ray lithography and other semiconductor processing techniques. Although these methods are capable of fabricating micro/nano structures with a resolution of less than a few nanometers, some of the shortcomings associated with these methods, such as high production costs for customized products, limited material choices, necessitate the development of other fabricating techniques. Micro/nano mechanical machining, such an atomic force microscope (AFM) probe based nano fabrication, has, therefore, been used to overcome some the major restrictions of the traditional processes. This technique removes material from the workpiece by engaging micro/nano size cutting tool (i.e. AFM probe) and is applicable on a wider range of materials compared to the photolithographic process. In spite of the unique benefits of nano mechanical machining, there are also some challenges with this technique, since the scale is reduced, such as size effects, burr formations, chip adhesions, fragility of tools and tool wear. Moreover, AFM based machining does not have any rotational movement, which makes fabrication of 3D features more difficult. Thus, vibration-assisted machining is introduced into AFM probe based nano mechanical machining to overcome the limitations associated with the conventional AFM probe based scratching method. Vibration-assisted machining reduced the cutting forces

  11. A Study on Remote On-Line Diagnostic System for Vehicles by Integrating the Technology of OBD, GPS, and 3G

    OpenAIRE

    Jyong Lin; Shih-Chang Chen; Yu-Tsen Shih; Shi-Huang Chen

    2009-01-01

    This paper presents a remote on-line diagnostic system for vehicles via the use of On-Board Diagnostic (OBD), GPS, and 3G techniques. The main parts of the proposed system are on-board computer, vehicle monitor server, and vehicle status browser. First, the on-board computer can obtain the location of deriver and vehicle status from GPS receiver and OBD interface, respectively. Then on-board computer will connect with the vehicle monitor server through 3G network to trans...

  12. Spectral Line Shapes. Proceedings

    International Nuclear Information System (INIS)

    Zoppi, M.; Ulivi, L.

    1997-01-01

    These proceedings represent papers presented at the 13th International Conference on Spectral Line Shapes which was held in Firenze,Italy from June 16-21, 1996. The topics covered a wide range of subjects emphasizing the physical processes associated with the formation of line profiles: high and low density plasma; atoms and molecules in strong laser fields, Dopple-free and ultra-fine spectroscopy; the line shapes generated by the interaction of neutrals, atoms and molecules, where the relavant quantities are single particle properties, and the interaction-induced spectroscopy. There were 131 papers presented at the conference, out of these, 6 have been abstracted for the Energy Science and Technology database

  13. Modular Rake of Pitot Probes

    Science.gov (United States)

    Dunlap, Timothy A.; Henry, Michael W.; Homyk, Raymond P.

    2004-01-01

    The figure presents selected views of a modular rake of 17 pitot probes for measuring both transient and steady-state pressures in a supersonic wind tunnel. In addition to pitot tubes visible in the figure, the probe modules contain (1) high-frequency dynamic-pressure transducers connected through wires to remote monitoring circuitry and (2) flow passages that lead to tubes that, in turn, lead to remote steady-state pressure transducers. Prior pitot-probe rakes were fabricated as unitary structures, into which the individual pitot probes were brazed. Repair or replacement of individual probes was difficult, costly, and time-consuming because (1) it was necessary to remove entire rakes in order to unbraze individual malfunctioning probes and (2) the heat of unbrazing a failed probe and of brazing a new probe in place could damage adjacent probes. In contrast, the modules in the present probe are designed to be relatively quickly and easily replaceable with no heating and, in many cases, without need for removal of the entire rake from the wind tunnel. To remove a malfunctioning probe, one first removes a screw-mounted V-cross-section cover that holds the probe and adjacent probes in place. Then one removes a screw-mounted cover plate to gain access to the steady-state pressure tubes and dynamicpressure wires. Next, one disconnects the tube and wires of the affected probe. Finally, one installs a new probe in the reverse of the aforementioned sequence. The wire connections can be made by soldering, but to facilitate removal and installation, they can be made via miniature plugs and sockets. The connections between the probe flow passages and the tubes leading to the remote pressure sensors can be made by use of any of a variety of readily available flexible tubes that can be easily pulled off and slid back on for removal and installation, respectively.

  14. Heavy ion beam probing

    International Nuclear Information System (INIS)

    Hickok, R.L.

    1980-07-01

    This report consists of the notes distributed to the participants at the IEEE Mini-Course on Modern Plasma Diagnostics that was held in Madison, Wisconsin in May 1980. It presents an overview of Heavy Ion Beam Probing that briefly describes the principles and discuss the types of measurements that can be made. The problems associated with implementing beam probes are noted, possible variations are described, estimated costs of present day systems, and the scaling requirements for large plasma devices are presented. The final chapter illustrates typical results that have been obtained on a variety of plasma devices. No detailed calculations are included in the report, but a list of references that will provide more detailed information is included

  15. Blinded Comparison between an In-Air Reverberation Method and an Electronic Probe Tester in the Detection of Ultrasound Probe Faults.

    Science.gov (United States)

    Dudley, Nicholas J; Woolley, Darren J

    2017-12-01

    The aim of this study was to perform a blinded trial, comparing the results of a visual inspection of the in-air reverberation pattern with the results of an electronic probe tester in detecting ultrasound probe faults. Sixty-two probes were tested. A total of 28 faults were found, 3 only by in-air reverberation assessment and 2 only by the electronic probe tester. The electronic probe tester provided additional information regarding the location of the fault in 74% of the cases in which both methods detected a fault. It is possible to detect the majority of probe faults by visual inspection and in-air reverberation assessment. The latter provides an excellent first-line test, easily performed on a daily basis by equipment users. An electronic probe tester is required if detailed evaluation of faults is necessary. Copyright © 2017 World Federation for Ultrasound in Medicine and Biology. All rights reserved.

  16. Probing lipid membrane electrostatics

    Science.gov (United States)

    Yang, Yi

    The electrostatic properties of lipid bilayer membranes play a significant role in many biological processes. Atomic force microscopy (AFM) is highly sensitive to membrane surface potential in electrolyte solutions. With fully characterized probe tips, AFM can perform quantitative electrostatic analysis of lipid membranes. Electrostatic interactions between Silicon nitride probes and supported zwitterionic dioleoylphosphatidylcholine (DOPC) bilayer with a variable fraction of anionic dioleoylphosphatidylserine (DOPS) were measured by AFM. Classical Gouy-Chapman theory was used to model the membrane electrostatics. The nonlinear Poisson-Boltzmann equation was numerically solved with finite element method to provide the potential distribution around the AFM tips. Theoretical tip-sample electrostatic interactions were calculated with the surface integral of both Maxwell and osmotic stress tensors on tip surface. The measured forces were interpreted with theoretical forces and the resulting surface charge densities of the membrane surfaces were in quantitative agreement with the Gouy-Chapman-Stern model of membrane charge regulation. It was demonstrated that the AFM can quantitatively detect membrane surface potential at a separation of several screening lengths, and that the AFM probe only perturbs the membrane surface potential by external field created by the internai membrane dipole moment. The analysis yields a dipole moment of 1.5 Debye per lipid with a dipole potential of +275 mV for supported DOPC membranes. This new ability to quantitatively measure the membrane dipole density in a noninvasive manner will be useful in identifying the biological effects of the dipole potential. Finally, heterogeneous model membranes were studied with fluid electric force microscopy (FEFM). Electrostatic mapping was demonstrated with 50 nm resolution. The capabilities of quantitative electrostatic measurement and lateral charge density mapping make AFM a unique and powerful

  17. Induced current heating probe

    International Nuclear Information System (INIS)

    Thatcher, G.; Ferguson, B.G.; Winstanley, J.P.

    1984-01-01

    An induced current heating probe is of thimble form and has an outer conducting sheath and a water flooded flux-generating unit formed from a stack of ferrite rings coaxially disposed in the sheath. The energising coil is made of solid wire which connects at one end with a coaxial water current tube and at the other end with the sheath. The stack of ferrite rings may include non-magnetic insulating rings which help to shape the flux. (author)

  18. Far Western: probing membranes.

    Science.gov (United States)

    Einarson, Margret B; Pugacheva, Elena N; Orlinick, Jason R

    2007-08-01

    INTRODUCTIONThe far-Western technique described in this protocol is fundamentally similar to Western blotting. In Western blots, an antibody is used to detect a query protein on a membrane. In contrast, in a far-Western blot (also known as an overlay assay) the antibody is replaced by a recombinant GST fusion protein (produced and purified from bacteria), and the assay detects the interaction of this protein with target proteins on a membrane. The membranes are washed and blocked, incubated with probe protein, washed again, and subjected to autoradiography. The GST fusion (probe) proteins are often labeled with (32)P; alternatively, the membrane can be probed with unlabeled GST fusion protein, followed by detection using commercially available GST antibodies. The nonradioactive approach is substantially more expensive (due to the purchase of antibody and detection reagents) than using radioactively labeled proteins. In addition, care must be taken to control for nonspecific interactions with GST alone and a signal resulting from antibody cross-reactivity. In some instances, proteins on the membrane are not able to interact after transfer. This may be due to improper folding, particularly in the case of proteins expressed from a phage expression library. This protocol describes a way to overcome this by washing the membrane in denaturation buffer, which is then serially diluted to permit slow renaturation of the proteins.

  19. Einstein Inflationary Probe (EIP)

    Science.gov (United States)

    Hinshaw, Gary

    2004-01-01

    I will discuss plans to develop a concept for the Einstein Inflation Probe: a mission to detect gravity waves from inflation via the unique signature they impart to the cosmic microwave background (CMB) polarization. A sensitive CMB polarization satellite may be the only way to probe physics at the grand-unified theory (GUT) scale, exceeding by 12 orders of magnitude the energies studied at the Large Hadron Collider. A detection of gravity waves would represent a remarkable confirmation of the inflationary paradigm and set the energy scale at which inflation occurred when the universe was a fraction of a second old. Even a strong upper limit to the gravity wave amplitude would be significant, ruling out many common models of inflation, and pointing to inflation occurring at much lower energy, if at all. Measuring gravity waves via the CMB polarization will be challenging. We will undertake a comprehensive study to identify the critical scientific requirements for the mission and their derived instrumental performance requirements. At the core of the study will be an assessment of what is scientifically and experimentally optimal within the scope and purpose of the Einstein Inflation Probe.

  20. The TMX heavy ion beam probe

    International Nuclear Information System (INIS)

    Hallock, G.A.

    1994-01-01

    A heavy ion beam probe has been used to measure the radial space potential distribution in the central cell of TMX. This was the first beam probe system to utilize computer control, CAMAC instrumentation, and fast time response for broadband fluctuation capabilities. The fast time response was obtained using off-line processing of the energy analyzer detector signals and wideband transimpedance amplifiers. The on-axis space potential was found to be 300--400 V, with φ e /T ec ∼8. The radial potential profile is parabolic when gas box fueling is used. The frequency of observed fluctuations was found to agree with the E x B plasma rotation frequency during the discharge. The measured Tl ++ secondary ion current level is consistent with calculations, given reasonable assumptions for beam attenuation

  1. Innovative technologies for emitter formation of crystalline silicon solar cells using in-line diffusion; Innovative Technologien zur Emittererzeugung fuer kristalline Silizium-Solarzellen mittels Durchlaufdiffusion

    Energy Technology Data Exchange (ETDEWEB)

    Voyer, Catherine

    2009-04-20

    An in-line emitter formation process for crystalline silicon solar cells was developed. The wafers were coated at room temperature with dilute phosphoric acid (2.5 w/w% in water) using ultrasonic spraying and then heated up to temperatures around 900 C in a metal-contamination-free in-line furnace. In the first zones of the furnace, a phosphosilicate glass (PSG) is formed on the silicon surface and serves as the doping source. The PSG thickness was adjusted by varying the flow rate of dilute phosphoric acid to the spray nozzle and took on values appropriate for emitter formation, in the range of {proportional_to}40-120 nm. A surfactant mixture was added to the dilute phosphoric acid in order to obtain complete wetting of the silicon surface. The mixture, which was composed of a hydrocarbon surfactant and of a fluorosurfactant, achieved better wetting properties than would be possible when using only one of the two surfactants. The spray solution containing only the hydrocarbon surfactant achieved a faster drop flattening, while the spray solution containing only the fluorosurfactant achieved a lower static surface tension. The mixture allowed for a combination of these desired properties: The drops coalesced together sufficiently rapidly (before drying) on the silicon surface to form a complete dopant source liquid layer and this layer remains sufficiently homogeneous during the layer drying. The sprayed-on layer is thicker ({proportional_to}15 microns) than the height of the surface texture ({proportional_to}5-10 microns). The liquid strives for a state of equilibrium, a convex meniscus. The topography of the liquid surface at the time at which the increase in viscosity puts an end to the liquid flow is reflected in the topography of the PSG thickness. The corresponding variations in sheet resistance across a wafer are sufficiently small for solar cells. Furthermore, the liquid layer conforms itself, during the drying, to the surface texture on a microscopic scale

  2. Image Processing in Optical Guidance for Autonomous Landing of Lunar Probe

    OpenAIRE

    Meng, Ding; Yun-feng, Cao; Qing-xian, Wu; Zhen, Zhang

    2008-01-01

    Because of the communication delay between earth and moon, the GNC technology of lunar probe is becoming more important than ever. Current navigation technology is not able to provide precise motion estimation for probe landing control system Computer vision offers a new approach to solve this problem. In this paper, author introduces an image process algorithm of computer vision navigation for autonomous landing of lunar probe. The purpose of the algorithm is to detect and track feature poin...

  3. Nine New Fluorescent Probes

    Science.gov (United States)

    Lin, Tsung-I.; Jovanovic, Misa V.; Dowben, Robert M.

    1989-06-01

    Absorption and fluorescence spectroscopic studies are reported here for nine new fluorescent probes recently synthesized in our laboratories: four pyrene derivatives with substituents of (i) 1,3-diacetoxy-6,8-dichlorosulfonyl, (ii) 1,3-dihydroxy-6,8-disodiumsulfonate, (iii) 1,3-disodiumsulfonate, and (iv) l-ethoxy-3,6,8-trisodiumsulfonate groups, and five [7-julolidino] coumarin derivatives with substituents of (v) 3-carboxylate-4-methyl, (vi) 3- methylcarboxylate, (vii) 3-acetate-4-methyl, (viii) 3-propionate-4-methyl, and (ix) 3-sulfonate-4-methyl groups. Pyrene compounds i and ii and coumarin compounds v and vi exhibit interesting absorbance and fluorescence properties: their absorption maxima are red shifted compared to the parent compound to the blue-green region, and the band width broadens considerably. All four blue-absorbing dyes fluoresce intensely in the green region, and the two pyrene compounds emit at such long wavelengths without formation of excimers. The fluorescence properties of these compounds are quite environment-sensitive: considerable spectral shifts and fluorescence intensity changes have been observed in the pH range from 3 to 10 and in a wide variety of polar and hydrophobic solvents with vastly different dielectric constants. The high extinction and fluorescence quantum yield of these probes make them ideal fluorescent labeling reagents for proteins, antibodies, nucleic acids, and cellular organelles. The pH and hydrophobicity-dependent fluorescence changes can be utilized as optical pH and/or hydrophobicity indicators for mapping environmental difference in various cellular components in a single cell. Since all nine probes absorb in the UV, but emit at different wavelengths in the visible, these two groups of compounds offer an advantage of utilizing a single monochromatic light source (e.g., a nitrogen laser) to achieve multi-wavelength detection for flow cytometry application. As a first step to explore potential application in

  4. Bottom lines

    International Nuclear Information System (INIS)

    Dabbs, F.

    1998-01-01

    The technological challenges facing the Alberta oilpatch in 1998 were reviewed. Attention was drawn to the fallacy of certain neo-conservative views, held by some members of the provincial government. This view holds that technology development is not a public concern and should be left entirely to the private sector. It is argued that rather than leaving it to the private sector, the government should play a strong leadership role. The government should do this by setting the agenda, by providing substantial and patient funding, by initiating partnerships with the private sector and by being a committed partner and catalyst in technology transfer. In support of this contention, the achievements of the Alberta Oil Sands Technology and Research Authority (AOSTRA) and of the Alberta Research Council were cite., These include the work on horizontal drilling, top-drive rigs, advanced exploration software, oilsands mining innovations, the steam-assisted gravity drainage (SAGD) technology, and the Taciuk process for bitumen recovery. All of these technologies, developed by government-industry funding, have contributed immeasurably to the Alberta economic miracle. Public expenditures on funding these research projects were but a fraction of the billions of dollars that have been, and will continue to be, returned to the people of Alberta in licence fees and royalties. In 1998, in the wake of the Kyoto Conference, the need for government leadership in energy research is even greater than it has been in the past. Nothing less than the viability of the fossil fuel industry and the Alberta economy depend upon finding solutions to the production and processing of greenhouse gas emissions

  5. Correlation between the sub-structure parameters and the manufacturing technologies of metal threads in historical textiles using X-ray line profile analysis

    Energy Technology Data Exchange (ETDEWEB)

    Csiszar, Gabor; Ungar, Tamas [Eoetvoes University Budapest, Department of Materials Physics, Budapest (Hungary); Jaro, Marta [Hungarian National Museum, Budapest (Hungary)

    2013-06-15

    Micro-structure can talk when documentation is missing. In ancient Roman or medieval periods, kings, queens, or just rich people decorated their clothes or even their horse covers richly with miniature jewels or metal threads. The origin or the fabrication techniques of these ancient threads is often unknown. Thirteen thread samples made of gold or gilt silver manufactured during the last sixteen hundred years are investigated for the micro-structure in terms of dislocation density, crystallite size, and planar defects. In a few cases, these features are compared with sub-structure of similar metallic threads prepared in modern, twentieth century workshops. The sub-structure is determined by X-ray line profile analysis, using high resolution diffractograms with negligible instrumental broadening. On the basis of the sub-structure parameters, we attempt to assess the metal-threads manufacturing procedures on samples stemming from the fourth century A.D. until now. (orig.)

  6. NIR technology for on-line determination of superficial a(w) and moisture content during the drying process of fermented sausages.

    Science.gov (United States)

    Collell, Carles; Gou, Pere; Arnau, Jacint; Muñoz, Israel; Comaposada, Josep

    2012-12-01

    Three different NIR equipment were evaluated based on their ability to predict superficial water activity (a(w)) and moisture content in two types of fermented sausages (with and without moulds on surface), using partial least squares (PLS) regression models. The instruments differed mainly in wavelength range, resolution and measurement configuration. The most accurate equipment was used in a new experiment to achieve robust models in sausages with different salt contents and submitted to different drying conditions. The models developed showed determination coefficients (R(2)(P)) values of 0.990, 0.910 and 0.984, and RMSEP values of 1.560%, 0.220% and 0.007% for moisture, salt and a(w) respectively. It was demonstrated that NIR spectroscopy could be a suitable non-destructive method for on-line monitoring and control of the drying process in fermented sausages. Copyright © 2012 Elsevier Ltd. All rights reserved.

  7. Wearable probes for service design

    DEFF Research Database (Denmark)

    Mullane, Aaron; Laaksolahti, Jarmo Matti; Svanæs, Dag

    2014-01-01

    Probes are used as a design method in user-centred design to allow end-users to inform design by collecting data from their lives. Probes are potentially useful in service innovation, but current probing methods require users to interrupt their activity and are consequently not ideal for use...... by service employees in reflecting on the delivery of a service. In this paper, we present the ‘wearable probe’, a probe concept that captures sensor data without distracting service employees. Data captured by the probe can be used by the service employees to reflect and co-reflect on the service journey......, helping to identify opportunities for service evolution and innovation....

  8. Transmission line capital costs

    International Nuclear Information System (INIS)

    Hughes, K.R.; Brown, D.R.

    1995-05-01

    The displacement or deferral of conventional AC transmission line installation is a key benefit associated with several technologies being developed with the support of the U.S. Department of Energy's Office of Energy Management (OEM). Previous benefits assessments conducted within OEM have been based on significantly different assumptions for the average cost per mile of AC transmission line. In response to this uncertainty, an investigation of transmission line capital cost data was initiated. The objective of this study was to develop a database for preparing preliminary estimates of transmission line costs. An extensive search of potential data sources identified databases maintained by the Bonneville Power Administration (BPA) and the Western Area Power Administration (WAPA) as superior sources of transmission line cost data. The BPA and WAPA data were adjusted to a common basis and combined together. The composite database covers voltage levels from 13.8 to 765 W, with cost estimates for a given voltage level varying depending on conductor size, tower material type, tower frame type, and number of circuits. Reported transmission line costs vary significantly, even for a given voltage level. This can usually be explained by variation in the design factors noted above and variation in environmental and land (right-of-way) costs, which are extremely site-specific. Cost estimates prepared from the composite database were compared to cost data collected by the Federal Energy Regulatory Commission (FERC) for investor-owned utilities from across the United States. The comparison was hampered because the only design specifications included with the FERC data were voltage level and line length. Working within this limitation, the FERC data were not found to differ significantly from the composite database. Therefore, the composite database was judged to be a reasonable proxy for estimating national average costs

  9. Recent developments in multimodality fluorescence imaging probes

    Directory of Open Access Journals (Sweden)

    Jianhong Zhao

    2018-05-01

    Full Text Available Multimodality optical imaging probes have emerged as powerful tools that improve detection sensitivity and accuracy, important in disease diagnosis and treatment. In this review, we focus on recent developments of optical fluorescence imaging (OFI probe integration with other imaging modalities such as X-ray computed tomography (CT, magnetic resonance imaging (MRI, positron emission tomography (PET, single-photon emission computed tomography (SPECT, and photoacoustic imaging (PAI. The imaging technologies are briefly described in order to introduce the strengths and limitations of each techniques and the need for further multimodality optical imaging probe development. The emphasis of this account is placed on how design strategies are currently implemented to afford physicochemically and biologically compatible multimodality optical fluorescence imaging probes. We also present studies that overcame intrinsic disadvantages of each imaging technique by multimodality approach with improved detection sensitivity and accuracy. KEY WORDS: Optical imaging, Fluorescence, Multimodality, Near-infrared fluorescence, Nanoprobe, Computed tomography, Magnetic resonance imaging, Positron emission tomography, Single-photon emission computed tomography, Photoacoustic imaging

  10. Fundamental size limitations of micro four-point probes

    DEFF Research Database (Denmark)

    Ansbæk, Thor; Petersen, Dirch Hjorth; Hansen, Ole

    2009-01-01

    The continued down-scaling of integrated circuits and magnetic tunnel junctions (MTJ) for hard disc read heads presents a challenge to current metrology technology. The four-point probes (4PP), currently used for sheet resistance characterization in these applications, therefore must be down......-scaled as well in order to correctly characterize the extremely thin films used. This presents a four-point probe design and fabrication challenge. We analyze the fundamental limitation on down-scaling of a generic micro four-point probe (M4PP) in a comprehensive study, where mechanical, thermal, and electrical...

  11. Novel Technology for Cloning Prostate Cancer Cell Markers

    National Research Council Canada - National Science Library

    Bancroft, F

    2002-01-01

    The purpose of the project is to employ probes isolated from the LNCaP series of human prostate cancer cells, to probe human cDNA microarrays, so as to investigate genes differentially expressed among these cell lines...

  12. production lines

    Directory of Open Access Journals (Sweden)

    Jingshan Li

    2000-01-01

    Full Text Available In this work, serial production lines with finished goods buffers operating in the pull regime are considered. The machines are assumed to obey Bernoulli reliability model. The problem of satisfying customers demand is addressed. The level of demand satisfaction is quantified by the due-time performance (DTP, which is defined as the probability to ship to the customer a required number of parts during a fixed time interval. Within this scenario, the definitions of DTP bottlenecks are introduced and a method for their identification is developed.

  13. Design and performance of low-wattage electrical heater probe

    International Nuclear Information System (INIS)

    Biddle, R.; Wetzel, J.R.; Cech, R.

    1997-01-01

    A mound electrical calibration heater (MECH) has been used in several EG and G Mound developed calorimeters as a calibration tool. They are very useful over the wattage range of a few to 500 W. At the lower end of the range, a bias develops between the MECH probe and calibrated heat standards. A low-wattage electrical calibration heater (L WECH) probe is being developed by the Safeguards Science and Technology group (NIS-5) of Los Alamos National Laboratory based upon a concept proposed by EG and G Mound personnel. The probe combines electrical resistive heating and laser-light powered heating. The LWECH probe is being developed for use with power settings up to 2W. The electrical heater will be used at the high end of the range, and laser-light power will be used low end of the wattage range. The system consists of two components: the heater probe and a control unit. The probe is inserted into the measuring cavity through an opening in the insulating baffle, and a sleeve is required to adapt to the measuring chamber. The probe is powered and controlled using electronics modules located separately. This paper will report on the design of the LWECH probe, initial tests, and expected performance

  14. Evaluation of Flat Surface Temperature Probes

    Science.gov (United States)

    Beges, G.; Rudman, M.; Drnovsek, J.

    2011-01-01

    The objective of this paper is elaboration of elements related to metrological analysis in the field of surface temperature measurement. Surface temperature measurements are applicable in many fields. As examples, safety testing of electrical appliances and a pharmaceutical production line represent case studies for surface temperature measurements. In both cases correctness of the result of the surface temperature has an influence on final product safety and quality and thus conformity with specifications. This paper deals with the differences of flat surface temperature probes in measuring the surface temperature. For the purpose of safety testing of electrical appliances, surface temperature measurements are very important for safety of the user. General requirements are presented in European standards, which support requirements in European directives, e.g., European Low Voltage Directive 2006/95/EC and pharmaceutical requirements, which are introduced in official state legislation. This paper introduces a comparison of temperature measurements of an attached thermocouple on the measured surface and measurement with flat surface temperature probes. As a heat generator, a so called temperature artifact is used. It consists of an aluminum plate with an incorporated electrical heating element with very good temperature stability in the central part. The probes and thermocouple were applied with different forces to the surface in horizontal and vertical positions. The reference temperature was measured by a J-type fine-wire (0.2 mm) thermocouple. Two probes were homemade according to requirements in the European standard EN 60335-2-9/A12, one with a fine-wire (0.2 mm) thermocouple and one with 0.5mm of thermocouple wire diameter. Additional commercially available probes were compared. Differences between probes due to thermal conditions caused by application of the probe were found. Therefore, it can happen that measurements are performed with improper equipment or

  15. Line facilities outline

    International Nuclear Information System (INIS)

    1998-08-01

    This book deals with line facilities. The contents of this book are outline line of wire telecommunication ; development of line, classification of section of line and theory of transmission of line, cable line ; structure of line, line of cable in town, line out of town, domestic cable and other lines, Optical communication ; line of optical cable, transmission method, measurement of optical communication and cable of the sea bottom, Equipment of telecommunication line ; telecommunication line facilities and telecommunication of public works, construction of cable line and maintenance and Regulation of line equipment ; regulation on technique, construction and maintenance.

  16. Design and Evaluation of Novel Polymyxin Fluorescent Probes

    Directory of Open Access Journals (Sweden)

    Bo Yun

    2017-11-01

    Full Text Available Polymyxins (polymyxin B and colistin are cyclic lipopeptide antibiotics that serve as a last-line defence against Gram-negative “superbugs”. In the present study, two novel fluorescent polymyxin probes were designed through regio-selective modifications of the polymyxin B core structure at the N-terminus and the hydrophobic motif at positions 6 and 7. The resulting probes, FADDI-285 and FADDI-286 demonstrated comparable antibacterial activity (MICs 2–8 mg/L to polymyxin B and colistin (MICs 0.5–8 mg/L against a panel of gram-negative clinical isolates of Acinetobacter baumannii, Klebsiella pneumoniae and Pseudomonas aeruginosa. These probes should prove to be of considerable utility for imaging cellular uptake and mechanistic investigations of these important last-line antibiotics.

  17. High spatial resolution Kelvin probe force microscopy with coaxial probes

    International Nuclear Information System (INIS)

    Brown, Keith A; Westervelt, Robert M; Satzinger, Kevin J

    2012-01-01

    Kelvin probe force microscopy (KPFM) is a widely used technique to measure the local contact potential difference (CPD) between an AFM probe and the sample surface via the electrostatic force. The spatial resolution of KPFM is intrinsically limited by the long range of the electrostatic interaction, which includes contributions from the macroscopic cantilever and the conical tip. Here, we present coaxial AFM probes in which the cantilever and cone are shielded by a conducting shell, confining the tip–sample electrostatic interaction to a small region near the end of the tip. We have developed a technique to measure the true CPD despite the presence of the shell electrode. We find that the behavior of these probes agrees with an electrostatic model of the force, and we observe a factor of five improvement in spatial resolution relative to unshielded probes. Our discussion centers on KPFM, but the field confinement offered by these probes may improve any variant of electrostatic force microscopy. (paper)

  18. Neutral helium beam probe

    Science.gov (United States)

    Karim, Rezwanul

    1999-10-01

    This article discusses the development of a code where diagnostic neutral helium beam can be used as a probe. The code solves numerically the evolution of the population densities of helium atoms at their several different energy levels as the beam propagates through the plasma. The collisional radiative model has been utilized in this numerical calculation. The spatial dependence of the metastable states of neutral helium atom, as obtained in this numerical analysis, offers a possible diagnostic tool for tokamak plasma. The spatial evolution for several hypothetical plasma conditions was tested. Simulation routines were also run with the plasma parameters (density and temperature profiles) similar to a shot in the Princeton beta experiment modified (PBX-M) tokamak and a shot in Tokamak Fusion Test Reactor tokamak. A comparison between the simulation result and the experimentally obtained data (for each of these two shots) is presented. A good correlation in such comparisons for a number of such shots can establish the accurateness and usefulness of this probe. The result can possibly be extended for other plasma machines and for various plasma conditions in those machines.

  19. Measuring the homogeneity of Bi(2223)/Ag tapes by four-probe method and a Hall probe array

    International Nuclear Information System (INIS)

    Kovac, P.

    1999-01-01

    The nature of the BSCCO compound and application of the powder-in-tube technique usually lead to non-uniform quality across and/or along the ceramic fibres and finally to variations in the critical current and its irregular distribution in the Bi(2223)/Ag tape. Therefore, the gliding four-probe method and contactless field monitoring measurements have been used for homogeneity studies. The gliding potential contacts moved along the tape surface and a sensitive system based on an integrated Hall probe array containing 16 or 19 in-line probes supported by PC-compatible electronics with software allowed us to make a comparison of contact and contactless measurements at any elements of Bi(2223)/Ag sample. The results of both methods show very good correlation and the possibility of using a sensitive Hall probe array for monitoring the final quality of Bi(2223)/Ag tapes. (author)

  20. CMOS Compatibility of a Micromachining Process Developed for Semiconductor Neural Probe

    National Research Council Canada - National Science Library

    An, S

    2001-01-01

    .... Test transistor patterns generated using standard CMOS fabrication line were exposed to a post-CMOS probe making process including dielectric deposition, gold metalization and the dry etching step...

  1. Probing cell mechanical properties with microfluidic devices

    Science.gov (United States)

    Rowat, Amy

    2012-02-01

    Exploiting flow on the micron-scale is emerging as a method to probe cell mechanical properties with 10-1000x advances in throughput over existing technologies. The mechanical properties of cells and the cell nucleus are implicated in a wide range of biological contexts: for example, the ability of white blood cells to deform is central to immune response; and malignant cells show decreased stiffness compared to benign cells. We recently developed a microfluidic device to probe cell and nucleus mechanical properties: cells are forced to deform through a narrow constrictions in response to an applied pressure; flowing cells through a series of constrictions enables us to probe the ability of hundreds of cells to deform and relax during flow. By tuning the constriction width so it is narrower than the width of the cell nucleus, we can specifically probe the effects of nuclear physical properties on whole cell deformability. We show that the nucleus is the rate-limiting step in cell passage: inducing a change in its shape to a multilobed structure results in cells that transit more quickly; increased levels of lamin A, a nuclear protein that is key for nuclear shape and mechanical stability, impairs the passage of cells through constrictions. We are currently developing a new class of microfluidic devices to simultaneously probe the deformability of hundreds of cell samples in parallel. Using the same soft lithography techniques, membranes are fabricated to have well-defined pore distribution, width, length, and tortuosity. We design the membranes to interface with a multiwell plate, enabling simultaneous measurement of hundreds of different samples. Given the wide spectrum of diseases where altered cell and nucleus mechanical properties are implicated, such a platform has great potential, for example, to screen cells based on their mechanical phenotype against a library of drugs.

  2. Manual of process automation. On-line control systems for devices in the process technology. 3. tot. rev. and enl. ed.; Handbuch der Prozessautomatisierung. Prozessleittechnik fuer verfahrenstechnische Anlagen

    Energy Technology Data Exchange (ETDEWEB)

    Maier, U.; Frueh, K.F. (eds.)

    2004-07-01

    This is a reference manual for engineers who need answers to automation problems in chemical engineering. Some new current subjects have been introduced to complement the information. The following chapters are new or have been rewritten by new authors: Internet and intranet technologies; Outline of process-related functions; Control systems in industrial applications; Problems and solutions; Model-based predicative control (MPC); Report archive analysis; Control Loop Performance Monitoring (CPM); Automation structures; Explosion protection; Remote-I/O; Integration of intelligent field equipment in PLS; Weighing and filling techniques; Safety; Maintenance - structures and strategies. The other chapters have been revised and updated as well. (orig.) [German] Das grundsaetzliche Konzept des Handbuchs ist unveraendert: Es dient als Nachschlagewerk fuer Ingenieure, die sich in verschiedenen Taetigkeitsbereichen mit Fragen der Automatisierung verfahrenstechnischer Anlagen auseinandersetzen muessen. Einige Themen wurden neu aufgenommen - wegen ihrer Aktualitaet und zur Abrundung des Themenspektrums. Folgende Kapitel sind voellig neu oder mit neuen Autoren wesentlich erweitert: Internet-/Intranettechnologien; Uebersicht ueber prozessnahe Funktionen; Industrielle Regelung: Probleme und Problemloesungen; Modellgestuetzte praediktive Regelung (MPC); Meldearchivanalyse; Control Loop Performance Monitoring (CPM); Automatisierungsstrukturen; Explosionsschutz; Remote-I/O; Integration intelligenter Feldgeraete in PLS; Waege- und Abfuelltechnik; Anlagensicherheit; Ganzheitliche Instandhaltung - Strukturen und Strategien. Die uebrigen Kapitel wurden aktualisiert und teilweise auch wesentlich ueberarbeitet. (orig.)

  3. Off-line wafer level reliability control: unique measurement method to monitor the lifetime indicator of gate oxide validated within bipolar/CMOS/DMOS technology

    Science.gov (United States)

    Gagnard, Xavier; Bonnaud, Olivier

    2000-08-01

    We have recently published a paper on a new rapid method for the determination of the lifetime of the gate oxide involved in a Bipolar/CMOS/DMOS technology (BCD). Because this previous method was based on a current measurement with gate voltage as a parameter needing several stress voltages, it was applied only by lot sampling. Thus, we tried to find an indicator in order to monitor the gate oxide lifetime during the wafer level parametric test and involving only one measurement of the device on each wafer test cell. Using the Weibull law and Crook model, combined with our recent model, we have developed a new test method needing only one electrical measurement of MOS capacitor to monitor the quality of the gate oxide. Based also on a current measurement, the parameter is the lifetime indicator of the gate oxide. From the analysis of several wafers, we gave evidence of the possibility to detect a low performance wafer, which corresponds to the infantile failure on the Weibull plot. In order to insert this new method in the BCD parametric program, a parametric flowchart was established. This type of measurement is an important challenges, because the actual measurements, breakdown charge, Qbd, and breakdown electric field, Ebd, at parametric level and Ebd and interface states density, Dit during the process cannot guarantee the gate oxide lifetime all along fabrication process. This indicator measurement is the only one, which predicts the lifetime decrease.

  4. The Antartic Ice Borehole Probe

    Science.gov (United States)

    Behar, A.; Carsey, F.; Lane, A.; Engelhardt, H.

    2000-01-01

    The Antartic Ice Borehole Probe mission is a glaciological investigation, scheduled for November 2000-2001, that will place a probe in a hot-water drilled hole in the West Antartic ice sheet. The objectives of the probe are to observe ice-bed interactions with a downward looking camera, and ice inclusions and structure, including hypothesized ice accretion, with a side-looking camera.

  5. Gravity Probe B Gyroscope Rotor

    Science.gov (United States)

    2003-01-01

    The Gravity Probe B (GP-B) is the relativity experiment developed at Stanford University to test two extraordinary predictions of Albert Einstein's general theory of relativity. The experiment will measure, very precisely, the expected tiny changes in the direction of the spin axes of four gyroscopes contained in an Earth-orbiting satellite at a 400-mile altitude. So free are the gyroscopes from disturbance that they will provide an almost perfect space-time reference system. They will measure how space and time are very slightly warped by the presence of the Earth, and, more profoundly, how the Earth's rotation very slightly drags space-time around with it. These effects, though small for the Earth, have far-reaching implications for the nature of matter and the structure of the Universe. This photograph is a close up of a niobium-coated gyroscope motor and its housing halves. GP-B is among the most thoroughly researched programs ever undertaken by NASA. This is the story of a scientific quest in which physicists and engineers have collaborated closely over many years. Inspired by their quest, they have invented a whole range of technologies that are already enlivening other branches of science and engineering. Launched April 20, 2004 , the GP-B program was managed for NASA by the Marshall Space Flight Center. Development of the GP-B is the responsibility of Stanford University along with major subcontractor Lockheed Martin Corporation. (Image credit to Don Harley.)

  6. Gravity Probe B Space Vehicle

    Science.gov (United States)

    2003-01-01

    The space vehicle for Gravity Probe B (GP-B) arrives at the launch site at Vandenburg Air Force Base. GP-B is the relativity experiment being developed at Stanford University to test two extraordinary predictions of Albert Einstein's general theory of relativity. The experiment will measure, very precisely, the expected tiny changes in the direction of the spin axes of four gyroscopes contained in an Earth-orbiting satellite at a 400-mile altitude. So free are the gyroscopes from disturbance that they will provide an almost perfect space-time reference system. They will measure how space and time are very slightly warped by the presence of the Earth, and, more profoundly, how the Earth's rotation very slightly drags space-time around with it. These effects, though small for the Earth, have far-reaching implications for the nature of matter and the structure of the Universe. GP-B is among the most thoroughly researched programs ever undertaken by NASA. This is the story of a scientific quest in which physicists and engineers have collaborated closely over many years. Inspired by their quest, they have invented a whole range of technologies that are already enlivening other branches of science and engineering. Scheduled for launch in 2003 and managed for NASA by the Marshall Space Flight Center, development of the GP-B is the responsibility of Stanford University, with major subcontractor Lockheed Martin Corporation.

  7. Limit lines for risk

    International Nuclear Information System (INIS)

    Cox, D.C.; Baybutt, P.

    1982-01-01

    Approaches to the regulation of risk from technological systems, such as nuclear power plants or chemical process plants, in which potential accidents may result in a broad range of adverse consequences must take into account several different aspects of risk. These include overall or average risk, accidents posing high relative risks, the rate at which accident probability decreases with increasing accident consequences, and the impact of high frequency, low consequence accidents. A hypothetical complementary cumulative distribution function (CCDF), with appropriately chosen parametric form, meets all these requirements. The Farmer limit line, by contrast, places limits on the risks due to individual accident sequences, and cannot adequately account for overall risk. This reduces its usefulness as a regulatory tool. In practice, the CCDF is used in the Canadian nuclear licensing process, while the Farmer limit line approach, supplemented by separate qualitative limits on overall risk, is employed in the United Kingdom

  8. Heat transfer probe

    Science.gov (United States)

    Frank, Jeffrey I.; Rosengart, Axel J.; Kasza, Ken; Yu, Wenhua; Chien, Tai-Hsin; Franklin, Jeff

    2006-10-10

    Apparatuses, systems, methods, and computer code for, among other things, monitoring the health of samples such as the brain while providing local cooling or heating. A representative device is a heat transfer probe, which includes an inner channel, a tip, a concentric outer channel, a first temperature sensor, and a second temperature sensor. The inner channel is configured to transport working fluid from an inner inlet to an inner outlet. The tip is configured to receive at least a portion of the working fluid from the inner outlet. The concentric outer channel is configured to transport the working fluid from the inner outlet to an outer outlet. The first temperature sensor is coupled to the tip, and the second temperature sensor spaced apart from the first temperature sensor.

  9. Solar Probe Plus

    Science.gov (United States)

    Szabo, Adam

    2011-01-01

    The NASA Solar Probe Plus mission is planned to be launched in 2018 to study the upper solar corona with both.in-situ and remote sensing instrumentation. The mission will utilize 6 Venus gravity assist maneuver to gradually lower its perihelion to 9.5 Rs below the expected Alfven pOint to study the sub-alfvenic solar wind that is still at least partially co-rotates with the Sun. The detailed science objectives of this mission will be discussed. SPP will have a strong synergy with The ESA/NASA Solar orbiter mission to be launched a year ahead. Both missions will focus on the inner heliosphere and will have complimentary instrumentations. Strategies to exploit this synergy will be also presented.

  10. Cosmological Probes for Supersymmetry

    Directory of Open Access Journals (Sweden)

    Maxim Khlopov

    2015-05-01

    Full Text Available The multi-parameter character of supersymmetric dark-matter models implies the combination of their experimental studies with astrophysical and cosmological probes. The physics of the early Universe provides nontrivial effects of non-equilibrium particles and primordial cosmological structures. Primordial black holes (PBHs are a profound signature of such structures that may arise as a cosmological consequence of supersymmetric (SUSY models. SUSY-based mechanisms of baryosynthesis can lead to the possibility of antimatter domains in a baryon asymmetric Universe. In the context of cosmoparticle physics, which studies the fundamental relationship of the micro- and macro-worlds, the development of SUSY illustrates the main principles of this approach, as the physical basis of the modern cosmology provides cross-disciplinary tests in physical and astronomical studies.

  11. Trapping and Probing Antihydrogen

    Energy Technology Data Exchange (ETDEWEB)

    Wurtele, Jonathan [UC Berkeley and LBNL

    2013-03-27

    Precision spectroscopy of antihydrogen is a promising path to sensitive tests of CPT symmetry. The most direct route to achieve this goal is to create and probe antihydrogen in a magnetic minimum trap. Antihydrogen has been synthesized and trapped for 1000s at CERN by the ALPHA Collaboration. Some of the challenges associated with achieving these milestones will be discussed, including mixing cryogenic positron and antiproton plasmas to synthesize antihydrogen with kinetic energy less than the trap potential of .5K. Recent experiments in which hyperfine transitions were resonantly induced with microwaves will be presented. The opportunity for gravitational measurements in traps based on detailed studies of antihydrogen dynamics will be described. The talk will conclude with a discussion future antihydrogen research that will use a new experimental apparatus, ALPHA-I.

  12. Traversing incore probe device

    International Nuclear Information System (INIS)

    Yoshioka, Michiko.

    1985-01-01

    Purpose: To measure the neutron flux distribution in the reactor core always at a high accuracy. Constitution: A nuclear fission ionizing chamber type detector is disposed at the end of a cable for sending a detection signal of a traversing incore probe device and, further, a gamma-ray ionizing chamber type detector is connected in adjacent therewith and a selection circuit for selecting both of the detection signals and inputting them to a display device is disposed. Then, compensation for the neutron monitors is conducted by the gamma-ray ionizing chamber type detector during normal operation in which control rods are not driven and the positioning is carried out by the nuclear fission ionizing chamber type detector. Furthermore, both of the compensation for the neutron detector and the positioning are carried out by the nuclear fission ionizing chamber type detector upon starting where the control rods are driven. (Sekiya, K.)

  13. High quality copy number and genotype data from FFPE samples using Molecular Inversion Probe (MIP) microarrays

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Yuker; Carlton, Victoria E.H.; Karlin-Neumann, George; Sapolsky, Ronald; Zhang, Li; Moorhead, Martin; Wang, Zhigang C.; Richardson, Andrea L.; Warren, Robert; Walther, Axel; Bondy, Melissa; Sahin, Aysegul; Krahe, Ralf; Tuna, Musaffe; Thompson, Patricia A.; Spellman, Paul T.; Gray, Joe W.; Mills, Gordon B.; Faham, Malek

    2009-02-24

    A major challenge facing DNA copy number (CN) studies of tumors is that most banked samples with extensive clinical follow-up information are Formalin-Fixed Paraffin Embedded (FFPE). DNA from FFPE samples generally underperforms or suffers high failure rates compared to fresh frozen samples because of DNA degradation and cross-linking during FFPE fixation and processing. As FFPE protocols may vary widely between labs and samples may be stored for decades at room temperature, an ideal FFPE CN technology should work on diverse sample sets. Molecular Inversion Probe (MIP) technology has been applied successfully to obtain high quality CN and genotype data from cell line and frozen tumor DNA. Since the MIP probes require only a small ({approx}40 bp) target binding site, we reasoned they may be well suited to assess degraded FFPE DNA. We assessed CN with a MIP panel of 50,000 markers in 93 FFPE tumor samples from 7 diverse collections. For 38 FFPE samples from three collections we were also able to asses CN in matched fresh frozen tumor tissue. Using an input of 37 ng genomic DNA, we generated high quality CN data with MIP technology in 88% of FFPE samples from seven diverse collections. When matched fresh frozen tissue was available, the performance of FFPE DNA was comparable to that of DNA obtained from matched frozen tumor (genotype concordance averaged 99.9%), with only a modest loss in performance in FFPE. MIP technology can be used to generate high quality CN and genotype data in FFPE as well as fresh frozen samples.

  14. Solar Probe: Humanity's First Visit to a Star

    Science.gov (United States)

    Hassler, D. M.; Solar Probe Science; Technology Definition Team

    Solar Probe will experience first hand the processes and conditions in the solar atmosphere that ultimately impact our planet and shape the harsh solar system environment It will be humanity s first visit to a star and will explore a previously inaccessible region of the inner heliosphere The 2003 Space Science Enterprise Strategy called for study of a Solar Probe to fly through the solar atmosphere to answer fundamental questions that can be answered in no other way The mission received highest priority in the National Academy of Sciences decadal research strategy in solar and space physics in 2002 Significant advances have been made in the areas of solar and solar wind science instrument technology mission resources and the mission environment since the previous Solar Probe Science Definition Team reports of 1989 1995 and 1999 The 2004-05 Solar Probe Science and Technology Definition Team STDT recently completed a detailed study of the Solar Probe Mission based on an earliest launch date of October 2014 The report and its Executive Summary were published by NASA in September 2005 and can be found at the website http solarprobe gsfc nasa gov This talk provides an overview of the Solar Probe mission and a summary of the efforts of the STDT

  15. Probe Selection for DNA Microarrays using OligoWiz

    DEFF Research Database (Denmark)

    Wernersson, Rasmus; Juncker, Agnieszka; Nielsen, Henrik Bjørn

    2007-01-01

    Nucleotide abundance measurements using DNA microarray technology are possible only if appropriate probes complementary to the target nucleotides can be identified. Here we present a protocol for selecting DNA probes for microarrays using the OligoWiz application. OligoWiz is a client-server appl......Nucleotide abundance measurements using DNA microarray technology are possible only if appropriate probes complementary to the target nucleotides can be identified. Here we present a protocol for selecting DNA probes for microarrays using the OligoWiz application. OligoWiz is a client......-server application that offers a detailed graphical interface and real-time user interaction on the client side, and massive computer power and a large collection of species databases (400, summer 2007) on the server side. Probes are selected according to five weighted scores: cross-hybridization, deltaT(m), folding...... computer skills and can be executed from any Internet-connected computer. The probe selection procedure for a standard microarray design targeting all yeast transcripts can be completed in 1 h....

  16. VT Digital Line Graph Miscellaneous Transmission Lines

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This datalayer is comprised of Miscellaineous Transmission Lines. Digital line graph (DLG) data are digital representations of cartographic...

  17. A comparison of alternative 60-mer probe designs in an in-situ synthesized oligonucleotide microarray

    Directory of Open Access Journals (Sweden)

    Fairbanks Benjamin D

    2006-04-01

    Full Text Available Abstract Background DNA microarrays have proven powerful for functional genomics studies. Several technologies exist for the generation of whole-genome arrays. It is well documented that 25mer probes directed against different regions of the same gene produce variable signal intensity values. However, the extent to which this is true for probes of greater length (60mers is not well characterized. Moreover, this information has not previously been reported for whole-genome arrays designed against bacteria, whose genomes may differ substantially in characteristics directly affecting microarray performance. Results We report here an analysis of alternative 60mer probe designs for an in-situ synthesized oligonucleotide array for the GC rich, β-proteobacterium Burkholderia cenocepacia. Probes were designed using the ArrayOligoSel3.5 software package and whole-genome microarrays synthesized by Agilent, Inc. using their in-situ, ink-jet technology platform. We first validated the quality of the microarrays as demonstrated by an average signal to noise ratio of >1000. Next, we determined that the variance of replicate probes (1178 total probes examined of identical sequence was 3.8% whereas the variance of alternative probes (558 total alternative probes examined designs was 9.5%. We determined that depending upon the definition, about 2.4% of replicate and 7.8% of alternative probes produced outlier conclusions. Finally, we determined none of the probe design subscores (GC content, internal repeat, binding energy and self annealment produced by ArrayOligoSel3.5 were predictive or probes that produced outlier signals. Conclusion Our analysis demonstrated that the use of multiple probes per target sequence is not essential for in-situ synthesized 60mer oligonucleotide arrays designed against bacteria. Although probes producing outlier signals were identified, the use of ratios results in less than 10% of such outlier conclusions. We also determined that

  18. Nanobits: customizable scanning probe tips

    DEFF Research Database (Denmark)

    Kumar, Rajendra; Shaik, Hassan Uddin; Sardan Sukas, Özlem

    2009-01-01

    We present here a proof-of-principle study of scanning probe tips defined by planar nanolithography and integrated with AFM probes using nanomanipulation. The so-called 'nanobits' are 2-4 mu m long and 120-150 nm thin flakes of Si3N4 or SiO2, fabricated by electron beam lithography and standard s...

  19. Gene probes: principles and protocols

    National Research Council Canada - National Science Library

    Aquino de Muro, Marilena; Rapley, Ralph

    2002-01-01

    ... of labeled DNA has allowed genes to be mapped to single chromosomes and in many cases to a single chromosome band, promoting significant advance in human genome mapping. Gene Probes: Principles and Protocols presents the principles for gene probe design, labeling, detection, target format, and hybridization conditions together with detailed protocols, accom...

  20. Non-inductive current probe

    DEFF Research Database (Denmark)

    Bak, Christen Kjeldahl

    1977-01-01

    The current probe described is a low-cost, shunt resistor for monitoring current pulses in e.g., pulsed lasers. Rise time is......The current probe described is a low-cost, shunt resistor for monitoring current pulses in e.g., pulsed lasers. Rise time is...

  1. Dr. Harry Whelan With the Light Emitting Diode Probe

    Science.gov (United States)

    1999-01-01

    The red light from the Light Emitting Diode (LED) probe shines through the fingers of Dr. Harry Whelan, a pediatric neurologist at the Children's Hospital of Wisconsin in Milwaukee. Dr. Whelan uses the long waves of light from the LED surgical probe to activate special drugs that kill brain tumors. Laser light previously has been used for this type of surgery, but the LED light illuminates through all nearby tissues, reaching parts of tumors that shorter wavelengths of laser light carnot. The new probe is safer because the longer wavelengths of light are cooler than the shorter wavelengths of laser light, making the LED less likely to injure normal brain tissue near the tumor. Also, it can be used for hours at a time while still remaining cool to the touch. The probe was developed for photodynamic cancer therapy under a NASA Small Business Innovative Research Program grant. The program is part of NASA's Technology Transfer Department at the Marshall Space Flight Center.

  2. Nanoscale microwave microscopy using shielded cantilever probes

    KAUST Repository

    Lai, Keji; Kundhikanjana, Worasom; Kelly, Michael A.; Shen, Zhi-Xun

    2011-01-01

    Quantitative dielectric and conductivity mapping in the nanoscale is highly desirable for many research disciplines, but difficult to achieve through conventional transport or established microscopy techniques. Taking advantage of the micro-fabrication technology, we have developed cantilever-based near-field microwave probes with shielded structures. Sensitive microwave electronics and finite-element analysis modeling are also utilized for quantitative electrical imaging. The system is fully compatible with atomic force microscope platforms for convenient operation and easy integration of other modes and functions. The microscope is ideal for interdisciplinary research, with demonstrated examples in nano electronics, physics, material science, and biology.

  3. Nanoscale microwave microscopy using shielded cantilever probes

    KAUST Repository

    Lai, Keji

    2011-04-21

    Quantitative dielectric and conductivity mapping in the nanoscale is highly desirable for many research disciplines, but difficult to achieve through conventional transport or established microscopy techniques. Taking advantage of the micro-fabrication technology, we have developed cantilever-based near-field microwave probes with shielded structures. Sensitive microwave electronics and finite-element analysis modeling are also utilized for quantitative electrical imaging. The system is fully compatible with atomic force microscope platforms for convenient operation and easy integration of other modes and functions. The microscope is ideal for interdisciplinary research, with demonstrated examples in nano electronics, physics, material science, and biology.

  4. Biomolecule recognition using piezoresistive nanomechanical force probes

    Science.gov (United States)

    Tosolini, Giordano; Scarponi, Filippo; Cannistraro, Salvatore; Bausells, Joan

    2013-06-01

    Highly sensitive sensors are one of the enabling technologies for the biomarker detection in early stage diagnosis of pathologies. We have developed a self-sensing nanomechanical force probe able for detecting the unbinding of single couples of biomolecular partners in nearly physiological conditions. The embedding of a piezoresistive transducer into a nanomechanical cantilever enabled high force measurement capability with sub 10-pN resolution. Here, we present the design, microfabrication, optimization, and complete characterization of the sensor. The exceptional electromechanical performance obtained allowed us to detect biorecognition specific events underlying the biotin-avidin complex formation, by integrating the sensor in a commercial atomic force microscope.

  5. Water cooled static pressure probe

    Science.gov (United States)

    Lagen, Nicholas T. (Inventor); Eves, John W. (Inventor); Reece, Garland D. (Inventor); Geissinger, Steve L. (Inventor)

    1991-01-01

    An improved static pressure probe containing a water cooling mechanism is disclosed. This probe has a hollow interior containing a central coolant tube and multiple individual pressure measurement tubes connected to holes placed on the exterior. Coolant from the central tube symmetrically immerses the interior of the probe, allowing it to sustain high temperature (in the region of 2500 F) supersonic jet flow indefinitely, while still recording accurate pressure data. The coolant exits the probe body by way of a reservoir attached to the aft of the probe. The pressure measurement tubes are joined to a single, larger manifold in the reservoir. This manifold is attached to a pressure transducer that records the average static pressure.

  6. Highly specific detection of genetic modification events using an enzyme-linked probe hybridization chip.

    Science.gov (United States)

    Zhang, M Z; Zhang, X F; Chen, X M; Chen, X; Wu, S; Xu, L L

    2015-08-10

    The enzyme-linked probe hybridization chip utilizes a method based on ligase-hybridizing probe chip technology, with the principle of using thio-primers for protection against enzyme digestion, and using lambda DNA exonuclease to cut multiple PCR products obtained from the sample being tested into single-strand chains for hybridization. The 5'-end amino-labeled probe was fixed onto the aldehyde chip, and hybridized with the single-stranded PCR product, followed by addition of a fluorescent-modified probe that was then enzymatically linked with the adjacent, substrate-bound probe in order to achieve highly specific, parallel, and high-throughput detection. Specificity and sensitivity testing demonstrated that enzyme-linked probe hybridization technology could be applied to the specific detection of eight genetic modification events at the same time, with a sensitivity reaching 0.1% and the achievement of accurate, efficient, and stable results.

  7. On-tip sub-micrometer Hall probes for magnetic microscopy prepared by AFM lithography

    International Nuclear Information System (INIS)

    Gregusova, D.; Martaus, J.; Fedor, J.; Kudela, R.; Kostic, I.; Cambel, V.

    2009-01-01

    We developed a technology of sub-micrometer Hall probes for future application in scanning hall probe microscopy (SHPM) and magnetic force microscopy (MFM). First, the Hall probes of ∼9-μm dimensions are prepared on the top of high-aspect-ratio GaAs pyramids with an InGaP/AlGaAs/GaAs active layer using wet-chemical etching and non-planar lithography. Then we show that the active area of planar Hall probes can be downsized to sub-micrometer dimensions by local anodic oxidation technique using an atomic force microscope. Such planar probes are tested and their noise and magnetic field sensitivity are evaluated. Finally, the two technologies are combined to fabricate sub-micrometer Hall probes on the top of high-aspect ratio mesa for future SHPM and MFM techniques.

  8. Application of Piezocomposite Twin, Side by Side, Phased Array UT Probes for the Inspection of Stainless Steel

    International Nuclear Information System (INIS)

    Delaide, M.; Dumas, Ph

    2005-01-01

    UT probes to be used for the examination of coarse-grain structure must allow to detect and size cracks, with a high reliability level. The combination of TRL probes, with phased array and piezocomposite technologies allows to improve probes performances and inspection speed. Single element crystals are replaced by matrix arrays, allowing to deflect and skew the beams, to change the inspection depth. This paper describes the designing, the manufacturing and the characterisation of several probes

  9. Steerable Doppler transducer probes

    International Nuclear Information System (INIS)

    Fidel, H.F.; Greenwood, D.L.

    1986-01-01

    An ultrasonic diagnostic probe is described which is capable of performing ultrasonic imaging and Doppler measurement consisting of: a hollow case having an acoustic window which passes ultrasonic energy and including chamber means for containing fluid located within the hollow case and adjacent to a portion of the acoustic window; imaging transducer means, located in the hollow case and outside the fluid chamber means, and oriented to direct ultrasonic energy through the acoustic window toward an area which is to be imaged; Doppler transducer means, located in the hollow case within the fluid chamber means, and movably oriented to direct Doppler signals through the acoustic window toward the imaged area; means located within the fluid chamber means and externally controlled for controllably moving the Doppler transducer means to select one of a plurality of axes in the imaged area along which the Doppler signals are to be directed; and means, located external to the fluid chamber means and responsive to the means for moving, for providing an indication signal for identifying the selected axis

  10. Coating process optimization through in-line monitoring for coating weight gain using Raman spectroscopy and design of experiments.

    Science.gov (United States)

    Kim, Byungsuk; Woo, Young-Ah

    2018-05-30

    In this study the authors developed a real-time Process Analytical Technology (PAT) of a coating process by applying in-line Raman spectroscopy to evaluate the coating weight gain, which is a quantitative analysis of the film coating layer. The wide area illumination (WAI) Raman probe was connected to the pan coater for real-time monitoring of changes in the weight gain of coating layers. Under the proposed in-line Raman scheme, a non-contact, non-destructive analysis was performed using WAI Raman probes with a spot size of 6 mm. The in-line Raman probe maintained a focal length of 250 mm, and a compressed air line was designed to protect the lens surface from spray droplets. The Design of Experiment (DOE) was applied to identify factors affecting the Raman spectra background of laser irradiation. The factors selected for DOE were the strength of compressed air connected to the probe, and the shielding of light by the transparent door connecting the probe to the pan coater. To develop a quantitative model, partial least squares (PLS) models as multivariate calibration were developed based on the three regions showing the specificity of TiO 2 individually or in combination. For the three single peaks (636 cm -1 , 512 cm -1 , 398 cm -1 ), least squares method (LSM) was applied to develop three univariate quantitative analysis models. One of best multivariate quantitative model having a factor of 1 gave the lowest RMSEP of 0.128, 0.129, and 0.125, respectively for prediction batches. When LSM was applied to the single peak at 636 cm -1 , the univariate quantitative model with an R 2 of 0.9863, slope of 0.5851, and y-intercept of 0.8066 had the lowest RMSEP of 0.138, 0.144, and 0.153, respectively for prediction batches. The in-line Raman spectroscopic method for the analysis of coating weight gain was verified by considering system suitability and parameters such as specificity, range, linearity, accuracy, and precision in accordance with ICH Q2 regarding

  11. The U-line line balancing problem

    NARCIS (Netherlands)

    Miltenburg, G.J.; Wijngaard, J.

    1994-01-01

    The traditional line balancing (LB) problem considers a production line in which stations are arranged consecutively in a line. A balance is determined by grouping tasks into stations while moving forward (or backward) through a precedence network. Recently many production lines are being arranged

  12. PEARS Emission Line Galaxies

    Science.gov (United States)

    Pirzkal, Nor; Rothberg, Barry; Ly, Chun; Rhoads, James E.; Malhotra, Sangeeta; Grogin, Norman A.; Dahlen, Tomas; Meurer, Gerhardt R.; Walsh, Jeremy; Hathi, Nimish P.; hide

    2012-01-01

    We present a full analysis of the Probing Evolution And Reionization Spectroscopically (PEARS) slitless grism spectroscopic data obtained vl'ith the Advanced Camera for Surveys on HST. PEARS covers fields within both the Great Observatories Origins Deep Survey (GOODS) North and South fields, making it ideal as a random surveY of galaxies, as well as the availability of a wide variety of ancillary observations to support the spectroscopic results. Using the PEARS data we are able to identify star forming galaxies within the redshift volume 0 galaxies down to a limiting flux of approx 10 - 18 erg/s/sq cm . The ELRs have also been compared to the properties of the host galaxy, including morphology, luminosity, and mass. From this analysis we find three key results: 1) The computed line luminosities show evidence of a flattening in the luminosity function with increasing redshift; 2) The star forming systems show evidence of disturbed morphologies, with star formation occurring predominantly within one effective (half-light) radius. However, the morphologies show no correlation with host stellar mass; and 3) The number density of star forming galaxies with M(*) >= 10(exp 9) Solar M decreases by an order of magnitude at z<=0.5 relative to the number at 0.5 < z < 0.9 in support of the argument for galaxy downsizing.

  13. SNAP: Small Next-generation Atmospheric Probe Concept

    Science.gov (United States)

    Sayanagi, K. M.; Dillman, R. A.; Atkinson, D. H.; Li, J.; Saikia, S.; Simon, A. A.; Spilker, T. R.; Wong, M. H.; Hope, D.

    2017-12-01

    We present a concept for a small, atmospheric probe that could be flexibly added to future missions that orbit or fly-by a giant planet as a secondary payload, which we call the Small Next-generation Atmospheric Probe (SNAP). SNAP's main scientific objectives are to determine the vertical distribution of clouds and cloud-forming chemical species, thermal stratification, and wind speed as a function of depth. As a case study, we present the advantages, cost and risk of adding SNAP to the future Uranus Orbiter and Probe flagship mission; in combination with the mission's main probe, SNAP would perform atmospheric in-situ measurements at a second location, and thus enable and enhance the scientific objectives recommended by the 2013 Planetary Science Decadal Survey and the 2014 NASA Science Plan to determine atmospheric spatial variabilities. We envision that the science objectives can be achieved with a 30-kg entry probe 0.5m in diameter (less than half the size of the Galileo probe) that reaches 5-bar pressure-altitude and returns data to Earth via the carrier spacecraft. As the baseline instruments, the probe will carry an Atmospheric Structure Instrument (ASI) that measures the temperature, pressure and acceleration, a carbon nanotube-based NanoChem atmospheric composition sensor, and an Ultra-Stable Oscillator (USO) to conduct a Doppler Wind Experiment (DWE). We also catalog promising technologies currently under development that will strengthen small atmospheric entry probe missions in the future. While SNAP is applicable to multiple planets, we examine the feasibility, benefits and impacts of adding SNAP to the Uranus Orbiter and Probe flagship mission. Our project is supported by NASA PSDS3 grant NNX17AK31G.

  14. Nanofabrication of magnetic scanned-probe microscope sensors

    International Nuclear Information System (INIS)

    Chong, B.K.

    2001-10-01

    This thesis presents the development of novel magnetic sensor combined with Atomic Force Microscope probe (AFM) using conventional semiconductor processing techniques and Electron Beam Lithography (EBL). The fabrication of these magnetic sensors was performed on a common micromachined silicon substrate using a generic batch fabrication technique. Sub-micron Hall bar for Scanning Hall probe Microscopy (SHPM) and electromagnetic force coil magnet for Scanning Electromagnetic Force Microscopy (eMFM) were designed and constructed at the apex of Silicon attractive mode cantilever probes. The process demonstrates good control over sensor parameters. Results indicated controllability of Hall bar junction sizes (spatial resolution) to below 100nm and Coil diameter sizes to below 500nm with minimum sizes down to 50nm and 270nm respectively. The process has shown its flexibility to accommodate different material systems. The same technology was used to fabricate multiple devices such as double Hall bars on a tip as well as a small electro-magnet coil probe co-defined with the Hall probe to form a magnetic imaging / modification probe. A conventional Non-Contact mode AFM employing heterodyne interferometry and in-house built electronics was modified for SHPM and eMFM. These probes had been scanned over a commercial computer hard disk. These microscopes showed the capability of resolving magnetic bits and topographic information independently and simultaneously. All scanning experiments were carried out under ambient conditions. The experiments required no extra preparation to be done to the specimen before imaging and measurements were carried out under ambient conditions. These probes offer the prospect of direct magnetic field measurement, non- invasiveness, very close proximity, possible local manipulation, better control over the tip- specimen interaction distance and topographic imaging. It is hoped that these magnetic microscope probes will be of great interest and

  15. Mini Tensiometer-Time Domain Reflectometry Coil Probe for Measuring Soil Water Retention Properties

    DEFF Research Database (Denmark)

    Subedi, Shaphal; Kawamoto, Ken; Karunarathna, Anurudda Kumara

    2013-01-01

    Time domain reflectometry (TDR) is used widely for measuring soil-water content. New TDR coil probe technology facilitates the development of small, nondestructive probes for simultaneous measurement of soil-water content (θ) and soil-water potential (ψ). In this study we developed mini tensiomet...... between measured soil-water retention curves (ψ > –100 cm H2O) by the new T-TDR coil probes and independent measurements by the hanging water column method....

  16. Aligned ion implementation using scanning probes

    Energy Technology Data Exchange (ETDEWEB)

    Persaud, A

    2006-12-12

    A new technique for precision ion implantation has been developed. A scanning probe has been equipped with a small aperture and incorporated into an ion beamline, so that ions can be implanted through the aperture into a sample. By using a scanning probe the target can be imaged in a non-destructive way prior to implantation and the probe together with the aperture can be placed at the desired location with nanometer precision. In this work first results of a scanning probe integrated into an ion beamline are presented. A placement resolution of about 120 nm is reported. The final placement accuracy is determined by the size of the aperture hole and by the straggle of the implanted ion inside the target material. The limits of this technology are expected to be set by the latter, which is of the order of 10 nm for low energy ions. This research has been carried out in the context of a larger program concerned with the development of quantum computer test structures. For that the placement accuracy needs to be increased and a detector for single ion detection has to be integrated into the setup. Both issues are discussed in this thesis. To achieve single ion detection highly charged ions are used for the implantation, as in addition to their kinetic energy they also deposit their potential energy in the target material, therefore making detection easier. A special ion source for producing these highly charged ions was used and their creation and interactions with solids of are discussed in detail. (orig.)

  17. Aligned ion implantation using scanning probes

    International Nuclear Information System (INIS)

    Persaud, A.

    2006-01-01

    A new technique for precision ion implantation has been developed. A scanning probe has been equipped with a small aperture and incorporated into an ion beamline, so that ions can be implanted through the aperture into a sample. By using a scanning probe the target can be imaged in a non-destructive way prior to implantation and the probe together with the aperture can be placed at the desired location with nanometer precision. In this work first results of a scanning probe integrated into an ion beamline are presented. A placement resolution of about 120 nm is reported. The final placement accuracy is determined by the size of the aperture hole and by the straggle of the implanted ion inside the target material. The limits of this technology are expected to be set by the latter, which is of the order of 10 nm for low energy ions. This research has been carried out in the context of a larger program concerned with the development of quantum computer test structures. For that the placement accuracy needs to be increased and a detector for single ion detection has to be integrated into the setup. Both issues are discussed in this thesis. To achieve single ion detection highly charged ions are used for the implantation, as in addition to their kinetic energy they also deposit their potential energy in the target material, therefore making detection easier. A special ion source for producing these highly charged ions was used and their creation and interactions with solids of are discussed in detail. (orig.)

  18. STM-SQUID probe microscope

    International Nuclear Information System (INIS)

    Hayashi, Tadayuki; Tachiki, Minoru; Itozaki, Hideo

    2007-01-01

    We have developed a STM-SQUID probe microscope. A high T C SQUID probe microscope was combined with a scanning tunneling microscope for investigation of samples at room temperature in air. A high permeability probe needle was used as a magnetic flux guide to improve the spatial resolution. The probe with tip radius of less than 100 nm was prepared by microelectropolishing. The probe was also used as a scanning tunneling microscope tip. Topography of the sample surface could be measured by the scanning tunneling microscope with high spatial resolution prior to observation by SQUID microscopy. The SQUID probe microscope image could be observed while keeping the distance from the sample surface to the probe tip constant. We observed a topographic image and a magnetic image of Ni fine pattern and also a magnetically recorded hard disk. Furthermore we have investigated a sample vibration method of the static magnetic field emanating from a sample with the aim of achieving a higher signal-to-noise (S/N) ratio

  19. The AMEMIYA probe. Theoretical background

    International Nuclear Information System (INIS)

    Belitz, Hans Joahim; Althausen, Bernhard; Uehara, Kazuya; Amemiya, Hiroshi

    2010-01-01

    The present probe was developed in order to measure the temperature T i of positive ions in the scrape-off layer (SOL) of tokamak where T i is usually larger than the electron temperature Ti so that the presheath in front of the probe need not be considered and the ions reach the probe with the thermal velocity. The axis of the cylindrical probe is placed parallel to the magnetic field. The important parameter are L/a, the ratio of the length to the radius of the cylindrical probe and κ, the ratio of the probe radius to (π/4) 1/2 , where is the mean ion Larmor radius. The ion current densities to the side and the end surfaces are expressed by the double integral, which can give an analytical formula with respect to the value of κ. If two electrodes with different lengths are placed parallel to the magnetic field, the difference of current densities can be reduced to κ and hence to Ti. Some examples of the application of the probe to tokamaks, JFT-2M and Textor, are demonstrated. (author)

  20. A PAT approach for the on-line monitoring of pharmaceutical co-crystals formation with near infrared spectroscopy.

    Science.gov (United States)

    Sarraguça, Mafalda C; Ribeiro, Paulo R S; Santos, Adenilson O; Silva, Marta C D; Lopes, João A

    2014-08-25

    Cocrystals represent a class of crystalline solids consisting of two or more molecular species usually held together by non-covalent bonds. Pharmaceutical cocrystals can alter the physicochemical properties of the active pharmaceutical ingredient to improve solubility, dissolution rate, particle properties and stability. This work presents a process analytical technology (PAT) approach to monitor on-line the cocrystallization of furosemide and adenine by solvent evaporation using near infrared spectroscopy (NIRS). Furosemide and adenine were added to a small volume of methanol in a beaker and stirred on an orbital stirring table during 8h at room temperature. The on-line monitoring was performed with a FT-NIR spectrometer fitted with a reflectance fiber optic probe. Monitoring was performed with the probe tip placed 1cm above the cocrystallization medium to avoid interference with the cocrystallization process. Cocrystals were vacuum dried to remove residual solvent and characterized off-line by NIRS, MIRS, DSC and XRPD. Results demonstrate that it was possible to follow the main cocrystallization events on-line. Copyright © 2014 Elsevier B.V. All rights reserved.

  1. Integrated microfluidic probe station.

    Science.gov (United States)

    Perrault, C M; Qasaimeh, M A; Brastaviceanu, T; Anderson, K; Kabakibo, Y; Juncker, D

    2010-11-01

    The microfluidic probe (MFP) consists of a flat, blunt tip with two apertures for the injection and reaspiration of a microjet into a solution--thus hydrodynamically confining the microjet--and is operated atop an inverted microscope that enables live imaging. By scanning across a surface, the microjet can be used for surface processing with the capability of both depositing and removing material; as it operates under immersed conditions, sensitive biological materials and living cells can be processed. During scanning, the MFP is kept immobile and centered over the objective of the inverted microscope, a few micrometers above a substrate that is displaced by moving the microscope stage and that is flushed continuously with the microjet. For consistent and reproducible surface processing, the gap between the MFP and the substrate, the MFP's alignment, the scanning speed, the injection and aspiration flow rates, and the image capture need all to be controlled and synchronized. Here, we present an automated MFP station that integrates all of these functionalities and automates the key operational parameters. A custom software program is used to control an independent motorized Z stage for adjusting the gap, a motorized microscope stage for scanning the substrate, up to 16 syringe pumps for injecting and aspirating fluids, and an inverted fluorescence microscope equipped with a charge-coupled device camera. The parallelism between the MFP and the substrate is adjusted using manual goniometer at the beginning of the experiment. The alignment of the injection and aspiration apertures along the scanning axis is performed using a newly designed MFP screw holder. We illustrate the integrated MFP station by the programmed, automated patterning of fluorescently labeled biotin on a streptavidin-coated surface.

  2. Short recovery time NMR probe

    International Nuclear Information System (INIS)

    Ramia, M.E.; Martin, C.A.; Jeandrevin, S.

    2011-01-01

    A NMR probe for low frequency and short recovery time is presented in this work. The probe contains the tuning circuit, diode expanders and quarter wavelength networks to protect the receiver from both the amplifier noise and the coil ringing following the transmitter power pulse. It also possesses a coil damper which is activated by of non active components. The probe performance shows a recovery time of about of 15μs a sensitive Q factor reduction and an increase of the signal to noise ratio of about 68% during the reception at a work frequency of 2 MHz. (author)

  3. Neurosurgery contact handheld probe based on sapphire shaped crystal

    Science.gov (United States)

    Shikunova, I. A.; Stryukov, D. O.; Rossolenko, S. N.; Kiselev, A. M.; Kurlov, V. N.

    2017-01-01

    A handheld contact probe based on sapphire shaped crystal is developed for intraoperative spectrally-resolved optical diagnostics, laser coagulation and aspiration of malignant brain tissue. The technology was integrated into the neurosurgical workflow for intraoperative real-time identification and removing of invasive brain cancer.

  4. NASA and international studies of the Solar Probe Mission

    Science.gov (United States)

    Randolph, James E.

    1992-01-01

    A review is presented summarizing the history and current status of the studies of the Solar Probe Mission by NASA and other space agencies. The technology and scientific challenges of the mission are addressed in these studies and can be met with current instrument and technology capabilities. The specific set of experiments recommended by a scientific advisory group to the NASA study for integration into the design concept is discussed.

  5. Touching is believing: interrogating halide perovskite solar cells at the nanoscale via scanning probe microscopy

    Science.gov (United States)

    Li, Jiangyu; Huang, Boyuan; Nasr Esfahani, Ehsan; Wei, Linlin; Yao, Jianjun; Zhao, Jinjin; Chen, Wei

    2017-10-01

    Halide perovskite solar cells based on CH3NH3PbI3 and related materials have emerged as the most exciting development in the next generation photovoltaic technologies, yet the microscopic phenomena involving photo-carriers, ionic defects, spontaneous polarization, and molecular vibration and rotation interacting with numerous grains, grain boundaries, and interfaces are still inadequately understood. In fact, there is still need for an effective method to interrogate the local photovoltaic properties of halide perovskite solar cells that can be directly traced to their microstructures on one hand and linked to their device performance on the other hand. In this perspective, we propose that scanning probe microscopy (SPM) techniques have great potential to realize such promises at the nanoscale, and highlight some of the recent progresses and challenges along this line of investigation toward local probing of photocurrent, work function, ionic activities, polarization switching, and chemical degradation. We also emphasize the importance of multi-modality imaging, in-operando scanning, big data analysis, and multidisciplinary collaboration for further studies toward fully understanding of these complex systems.

  6. Electromagnetic microscope compared with a conventional pulsed eddy-current probe

    Science.gov (United States)

    Podney, Walter N.

    1998-03-01

    A superconductive probe presently can detect a crack at a rivet hole that is two to three times smaller than the smallest crack detectable by a conventional probe. As the technology matures and noise resolution approaches a limit set by SQUIDS, approximately 1 fH, it will enable detecting submillimeter cracks down to approximately 15 mm.

  7. Using Downhole Probes to Locate and Characterize Buried Transuranic and Mixed Low Level Waste

    Energy Technology Data Exchange (ETDEWEB)

    Steinman, Donald K; Bramblett, Richard L; Hertzog, Russel C

    2012-06-25

    Borehole logging probes were developed and tested to locate and quantify transuranic elements in subsurface disposal areas and in contaminated sites at USDOE Weapons Complex sites. A new method of measuring very high levels of chlroine in the subsurface was developed using pulsed neutron technology from oilfield applications. The probes were demonstrated at the Hanford site in wells containing plutonium and other contaminants.

  8. Accuracy enhancement of point triangulation probes for linear displacement measurement

    Science.gov (United States)

    Kim, Kyung-Chan; Kim, Jong-Ahn; Oh, SeBaek; Kim, Soo Hyun; Kwak, Yoon Keun

    2000-03-01

    Point triangulation probes (PTBs) fall into a general category of noncontact height or displacement measurement devices. PTBs are widely used for their simple structure, high resolution, and long operating range. However, there are several factors that must be taken into account in order to obtain high accuracy and reliability; measurement errors from inclinations of an object surface, probe signal fluctuations generated by speckle effects, power variation of a light source, electronic noises, and so on. In this paper, we propose a novel signal processing algorithm, named as EASDF (expanded average square difference function), for a newly designed PTB which is composed of an incoherent source (LED), a line scan array detector, a specially selected diffuse reflecting surface, and several optical components. The EASDF, which is a modified correlation function, is able to calculate displacement between the probe and the object surface effectively even if there are inclinations, power fluctuations, and noises.

  9. A particle accelerator probes artifacts

    International Nuclear Information System (INIS)

    Dran, J.C.; Calligaro, Th.; Salomon, J.

    2002-01-01

    The AGLAE system is made up of a 2 mega volts electrostatic accelerator and of 3 irradiation lines: one leads to a vacuum enclosure in which targets are irradiated and the 2 others lines are designed to irradiate targets under an air or helium atmosphere. The AGLAE system is located in the premises of the Louvre museum in Paris and is devoted to the study of cultural objects through ion beam analysis (IBA). 4 techniques are used: -) proton-induced X-ray emission (PIXE) -) proton-induced gamma ray (PIGE) -) Rutherford backscattering spectrometry (NRS) and -) nuclear reaction analysis (NRA). A decisive progress has permitted the direct analysis of artifacts without sampling. The object itself is set just a few millimeters away from the exit window of the beam in an air or helium atmosphere. The exit window must be resistant enough to bear the atmospheric pressure and the damages caused by the proton beam but must be thin enough to not deteriorate the quality of the beam. By using a 10 -7 m thick exit window made of Si 3 N 4 we get a beam whose diameter is 10 -5 m. This new technology presents 4 main advantages: 1) any object of any shape can be studied without sampling, 2) the analysis of very fragile artifacts that might be damaged by the vacuum setting is now possible, 3) a reduction of the thermal side-effects of the beam, and 4) the absence of accumulation of charges in isolating material allows to rid of covering the object with a conducting coating before irradiating it. (A.C.)

  10. A Common Probe Design for Multiple Planetary Destinations

    Science.gov (United States)

    Hwang, H. H.; Allen, G. A., Jr.; Alunni, A. I.; Amato, M. J.; Atkinson, D. H.; Bienstock, B. J.; Cruz, J. R.; Dillman, R. A.; Cianciolo, A. D.; Elliott, J. O.; hide

    2018-01-01

    Atmospheric probes have been successfully flown to planets and moons in the solar system to conduct in situ measurements. They include the Pioneer Venus multi-probes, the Galileo Jupiter probe, and Huygens probe. Probe mission concepts to five destinations, including Venus, Jupiter, Saturn, Uranus, and Neptune, have all utilized similar-shaped aeroshells and concept of operations, namely a 45-degree sphere cone shape with high density heatshield material and parachute system for extracting the descent vehicle from the aeroshell. Each concept designed its probe to meet specific mission requirements and to optimize mass, volume, and cost. At the 2017 International Planetary Probe Workshop (IPPW), NASA Headquarters postulated that a common aeroshell design could be used successfully for multiple destinations and missions. This "common probe"� design could even be assembled with multiple copies, properly stored, and made available for future NASA missions, potentially realizing savings in cost and schedule and reducing the risk of losing technologies and skills difficult to sustain over decades. Thus the NASA Planetary Science Division funded a study to investigate whether a common probe design could meet most, if not all, mission needs to the five planetary destinations with extreme entry environments. The Common Probe study involved four NASA Centers and addressed these issues, including constraints and inefficiencies that occur in specifying a common design. Study methodology: First, a notional payload of instruments for each destination was defined based on priority measurements from the Planetary Science Decadal Survey. Steep and shallow entry flight path angles (EFPA) were defined for each planet based on qualification and operational g-load limits for current, state-of-the-art instruments. Interplanetary trajectories were then identified for a bounding range of EFPA. Next, 3-degrees-of-freedom simulations for entry trajectories were run using the entry state

  11. Lepton probes in nuclear physics

    Energy Technology Data Exchange (ETDEWEB)

    Arvieux, J. [Laboratoire National Saturne, Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France)

    1994-12-31

    Facilities are overviewed which use the lepton probe to learn about nuclear physics. The lepton accelerating methods out some existing facilities are considered. The ELFE project is discussed in detail. (K.A.). 43 refs., 15 figs., 4 tabs.

  12. Probing of flowing electron plasmas

    International Nuclear Information System (INIS)

    Himura, H.; Nakashima, C.; Saito, H.; Yoshida, Z.

    2001-01-01

    Probing of streaming electron plasmas with finite temperature is studied. For the first time, a current-voltage characteristic of an electric probe is measured in electron plasmas. Due to the fast flow of the electron plasmas, the characteristic curve spreads out significantly and exhibits a long tail. This feature can be explained calculating the currents collected to the probe. In flowing electron plasmas, the distribution function observed in the laboratory frame is non-Maxwellian even if the plasmas come to a state of thermal equilibrium. Another significant feature of the characteristic is that it determines a floating potential where the current equals zero, despite there being very few ions in the electron plasma. A high impedance probe, which is popularly used to determine the space potential of electron plasmas, outputs the potential. The method is available only for plasmas with density much smaller than the Brillouin limit

  13. Monitoring probe for groundwater flow

    Science.gov (United States)

    Looney, B.B.; Ballard, S.

    1994-08-23

    A monitoring probe for detecting groundwater migration is disclosed. The monitor features a cylinder made of a permeable membrane carrying an array of electrical conductivity sensors on its outer surface. The cylinder is filled with a fluid that has a conductivity different than the groundwater. The probe is placed in the ground at an area of interest to be monitored. The fluid, typically saltwater, diffuses through the permeable membrane into the groundwater. The flow of groundwater passing around the permeable membrane walls of the cylinder carries the conductive fluid in the same general direction and distorts the conductivity field measured by the sensors. The degree of distortion from top to bottom and around the probe is precisely related to the vertical and horizontal flow rates, respectively. The electrical conductivities measured by the sensors about the outer surface of the probe are analyzed to determine the rate and direction of the groundwater flow. 4 figs.

  14. Pneumatic probe with laser interferometer

    International Nuclear Information System (INIS)

    Wilkens, P.H.

    1978-01-01

    Improvements to upgrade the accuracy of Rotacon probes by a complete redesign of probe to include a Michelson interferometer to replace the existing long-range capacity transducer are described. This has resulted in a compact and interchangeable probe cartridge with a 3 μin. resolution and accuracy; the cartridge can be installed and replaced in the Rotacon gauge with the minimum of realignment, which should reduce our dependence on operator skill. In addition, the stylus contact force can be reduced to 750 mg for the contacting types, but an alternative feature, which we are still developing, will use a gas jet cushion in place of the stylus to provide a noncontacting version of the same basic probe cartridge. This device is very sensitive to external vibration effects because it is virtually frictionless

  15. Lepton probes in nuclear physics

    International Nuclear Information System (INIS)

    Arvieux, J.

    1994-01-01

    Facilities are overviewed which use the lepton probe to learn about nuclear physics. The lepton accelerating methods out some existing facilities are considered. The ELFE project is discussed in detail. (K.A.). 43 refs., 15 figs., 4 tabs

  16. Harnessing natural product assembly lines: structure, promiscuity, and engineering.

    Science.gov (United States)

    Ladner, Christopher C; Williams, Gavin J

    2016-03-01

    Many therapeutically relevant natural products are biosynthesized by the action of giant mega-enzyme assembly lines. By leveraging the specificity, promiscuity, and modularity of assembly lines, a variety of strategies has been developed that enables the biosynthesis of modified natural products. This review briefly summarizes recent structural advances related to natural product assembly lines, discusses chemical approaches to probing assembly line structures in the absence of traditional biophysical data, and surveys efforts that harness the inherent or engineered promiscuity of assembly lines for the synthesis of non-natural polyketides and non-ribosomal peptide analogues.

  17. DNA probe for lactobacillus delbrueckii

    Energy Technology Data Exchange (ETDEWEB)

    Delley, M.; Mollet, B.; Hottinger, H. (Nestle Research Centre, Lausanne (Switzerland))

    1990-06-01

    From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific DNA probe. Dot-blot hybridizations with over 40 different Lactobacillus strains showed that this clone specifically recognized L. delbrueckii subsp. delbrueckii, bulgaricus, and lactis. The sensitivity of the method was tested by using an {alpha}-{sup 32}P-labeled probe.

  18. DNA probe for lactobacillus delbrueckii

    International Nuclear Information System (INIS)

    Delley, M.; Mollet, B.; Hottinger, H.

    1990-01-01

    From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific DNA probe. Dot-blot hybridizations with over 40 different Lactobacillus strains showed that this clone specifically recognized L. delbrueckii subsp. delbrueckii, bulgaricus, and lactis. The sensitivity of the method was tested by using an α- 32 P-labeled probe

  19. Deoxyribonucleic Acid Probes Analyses for the Detection of Periodontal Pathogens.

    Science.gov (United States)

    Al Yahfoufi, Zoubeida; Hadchiti, Wahib; Berberi, Antoine

    2015-09-01

    In clinical microbiology several techniques have been used to identify bacteria. Recently, Deoxyribonucleic acid (DNA)-based techniques have been introduced to detect human microbial pathogens in periodontal diseases. Deoxyribonucleic acid probes can detect bacteria at a very low level if we compared with the culture methods. These probes have shown rapid and cost-effective microbial diagnosis, good sensitivity and specificity for some periodontal pathogens in cases of severe periodontitis. Eighty-five patients were recruited for the study. Twenty-one subjects ranging between 22 and 48 years of age fulfilled the inclusion and exclusion criteria. Seventy-eight samples became available for DNA probe analysis from the deepest pockets in each quadrant. All 21 patients showed positive results for Prevotella intermedia; also, Prevotella gingivalis was identified in 19 subjects, Aggregatibacter actinomycetemcomitans in 6 subjects. P. intermedia was diagnosed positive in 82% of the subgingival samples taken, 79% for P. gingivalis, and 23% for A. actinomycetemcomitans. This study shows a high frequency of putative periodontal pathogens by using DNA probe technology, which is semi-quantitative in this study. Deoxyribonucleic acid probes can detect bacteria at very low level about 10(3) which is below the detection level of culture methods. The detection threshold of cultural methods. The three types of bacteria can be detected rapidly with high sensitivity by using the DNA probe by general practitioners, and thus can help in the diagnosis process and the treatment.

  20. Seven novel probe systems for real-time PCR provide absolute single-base discrimination, higher signaling, and generic components.

    Science.gov (United States)

    Murray, James L; Hu, Peixu; Shafer, David A

    2014-11-01

    We have developed novel probe systems for real-time PCR that provide higher specificity, greater sensitivity, and lower cost relative to dual-labeled probes. The seven DNA Detection Switch (DDS)-probe systems reported here employ two interacting polynucleotide components: a fluorescently labeled probe and a quencher antiprobe. High-fidelity detection is achieved with three DDS designs: two internal probes (internal DDS and Flip probes) and a primer probe (ZIPR probe), wherein each probe is combined with a carefully engineered, slightly mismatched, error-checking antiprobe. The antiprobe blocks off-target detection over a wide range of temperatures and facilitates multiplexing. Other designs (Universal probe, Half-Universal probe, and MacMan probe) use generic components that enable low-cost detection. Finally, single-molecule G-Force probes employ guanine-mediated fluorescent quenching by forming a hairpin between adjacent C-rich and G-rich sequences. Examples provided show how these probe technologies discriminate drug-resistant Mycobacterium tuberculosis mutants, Escherichia coli O157:H7, oncogenic EGFR deletion mutations, hepatitis B virus, influenza A/B strains, and single-nucleotide polymorphisms in the human VKORC1 gene. Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

  1. A practical model for pressure probe system response estimation (with review of existing models)

    Science.gov (United States)

    Hall, B. F.; Povey, T.

    2018-04-01

    The accurate estimation of the unsteady response (bandwidth) of pneumatic pressure probe systems (probe, line and transducer volume) is a common practical problem encountered in the design of aerodynamic experiments. Understanding the bandwidth of the probe system is necessary to capture unsteady flow features accurately. Where traversing probes are used, the desired traverse speed and spatial gradients in the flow dictate the minimum probe system bandwidth required to resolve the flow. Existing approaches for bandwidth estimation are either complex or inaccurate in implementation, so probes are often designed based on experience. Where probe system bandwidth is characterized, it is often done experimentally, requiring careful experimental set-up and analysis. There is a need for a relatively simple but accurate model for estimation of probe system bandwidth. A new model is presented for the accurate estimation of pressure probe bandwidth for simple probes commonly used in wind tunnel environments; experimental validation is provided. An additional, simple graphical method for air is included for convenience.

  2. A 40 GHz fully integrated circuit with a vector network analyzer and a coplanar-line-based detection area for circulating tumor cell analysis using 65 nm CMOS technology

    Science.gov (United States)

    Nakanishi, Taiki; Matsunaga, Maya; Kobayashi, Atsuki; Nakazato, Kazuo; Niitsu, Kiichi

    2018-03-01

    A 40-GHz fully integrated CMOS-based circuit for circulating tumor cells (CTC) analysis, consisting of an on-chip vector network analyzer (VNA) and a highly sensitive coplanar-line-based detection area is presented in this paper. In this work, we introduce a fully integrated architecture that eliminates unwanted parasitic effects. The proposed analyzer was designed using 65 nm CMOS technology, and SPICE and MWS simulations were used to validate its operation. The simulation confirmed that the proposed circuit can measure S-parameter shifts resulting from the addition of various types of tumor cells to the detection area, the data of which are provided in a previous study: the |S 21| values for HepG2, A549, and HEC-1-A cells are -0.683, -0.580, and -0.623 dB, respectively. Additionally, the measurement demonstrated an S-parameters reduction of -25.7% when a silicone resin was put on the circuit. Hence, the proposed system is expected to contribute to cancer diagnosis.

  3. Improving technology and setting-up a production line for high quality zinc oxide (99.5%) with a capacity of 150 ton/year by evaporation-oxidation process

    International Nuclear Information System (INIS)

    Phan Dinh Thinh; Pham Minh Tuan; Luong Manh Hung; Tran Ngoc Vuong

    2015-01-01

    This report presents the technology improvement and a production line to produce high quality zinc oxide of purity upper than 99.5% ZnO by evaporation-oxidation method. Secondary zinc metal recovered from galvanizing industrial will undergo a pre-treatment to meet all requirements of standardized feed material for evaporation-oxidation process. Zinc metal is melted at a temperature of about 650"oC, some impurities and metallic oxides are separated preliminary, then zinc metal is converted into liquid in evaporation pot. Here the temperature is maintained around 1050"oC, zinc liquid is evaporated, zinc vapor is oxidized by air in the oxidation chamber naturally by oxygen in the air and then zinc vapor is converted to zinc oxide. Zinc oxide is passed through a product classification systems and then go to a product collection of filtering bag design. The whole process of melting, evaporation, oxidation, particles classification and product collection is a continuous process. The efficiency of the transformation of zinc metal into zinc oxide can reach the value of 1.1 to 1.2. ZnO product quality is higher than 99.5%. (author)

  4. Probing Extragalactic Planets Using Quasar Microlensing

    Science.gov (United States)

    Dai, Xinyu; Guerras, Eduardo

    2018-02-01

    Previously, planets have been detected only in the Milky Way galaxy. Here, we show that quasar microlensing provides a means to probe extragalactic planets in the lens galaxy, by studying the microlensing properties of emission close to the event horizon of the supermassive black hole of the background quasar, using the current generation telescopes. We show that a population of unbound planets between stars with masses ranging from Moon to Jupiter masses is needed to explain the frequent Fe Kα line energy shifts observed in the gravitationally lensed quasar RXJ 1131–1231 at a lens redshift of z = 0.295 or 3.8 billion lt-yr away. We constrain the planet mass-fraction to be larger than 0.0001 of the halo mass, which is equivalent to 2000 objects ranging from Moon to Jupiter mass per main-sequence star.

  5. Power line communications theory and applications for narrowband and broadband communications over power lines

    CERN Document Server

    Ferreira, Hendrik C; Newbury, John; Swart, Theo G

    2010-01-01

    Power Line Communications (PLC) is a promising emerging technology, which has attracted much attention due to the wide availability of power distribution lines. This book provides a thorough introduction to the use of power lines for communication purposes, ranging from channel characterization, communications on the physical layer and electromagnetic interference, through to protocols, networks, standards and up to systems and implementations. With contributions from many of the most prominent international PLC experts from academia and industry, Power Line Communications brings togeth

  6. Positron annihilation probing for the hydratation rate of cement paste

    International Nuclear Information System (INIS)

    Myllylae, R.; Karras, M.

    1975-01-01

    Positron annihilation has been found to be a possible probe for the exponential hydratation of cement paste. Both lifetime and Doppler line broadening measurements revealed the hydratation rate. With the aid of increased stability in the lifetime spectrometer it has been possible to extend the measuring sensitivity over a period of several weeks. Two main lifetimes, tau 1 = 480 +- 20 psec and tau 2 = 2.1 +- 0.1 nsec, were observed to be constant during the hydratation. The intensity of the 2.1 nsec component changed from 4 to 8% after 47 days, and simultaneously the annihilation line narrowed from 2.6 to 2.4 keV. This behaviour has been interpreted as an increase in positronium formation. The possible practical applications of positron annihilation radiation as a hydratation probe has been evaluated for use in a concrete laboratory and even for regular construction work. (orig.) [de

  7. PROBES, POPULATIONS, SAMPLES, MEASUREMENTS AND RELATIONS IN STEREOLOGY

    Directory of Open Access Journals (Sweden)

    Robert T Dehoff

    2011-05-01

    Full Text Available This summary paper provides an overview of the content of stereology. The typical problem at hand centers around some three dimensional object that has an internal structure that determines its function, performance, or response. To understand and quantify the geometry of that structure it is necessary to probe it with geometric entities: points, lines, planes volumes, etc. Meaningful results are obtained only if the set of probes chosen for use in the assessment is drawn uniformly from the population of such probes for the structure as a whole. This requires an understanding of the population of each kind of probe. Interaction of the probes with the structure produce geometric events which are the focus of stereological measurements. In almost all applications the measurement that is made is a simple count of the number of these events. Rigorous application of these requirements for sample design produce unbiased estimates of geometric properties of features in the structure no matter how complex are the features or what their arrangement in space. It is this assumption-free characteristic of the methodology that makes it a powerful tool for characterizing the internal structure of three dimensional objects.

  8. High precision capacitive beam phase probe for KHIMA project

    Energy Technology Data Exchange (ETDEWEB)

    Hwang, Ji-Gwang, E-mail: windy206@hanmail.net [Korea Institute of Radiological and Medical Sciences, 215–4, Gongneung-dong, Nowon-t, Seoul 139–706 (Korea, Republic of); Yang, Tae-Keun [Korea Institute of Radiological and Medical Sciences, 215–4, Gongneung-dong, Nowon-t, Seoul 139–706 (Korea, Republic of); Forck, Peter [GSI Helmholtz Centre for Ion Research, Darmstadt 64291, German (Germany)

    2016-11-21

    In the medium energy beam transport (MEBT) line of KHIMA project, a high precision beam phase probe monitor is required for a precise tuning of RF phase and amplitude of Radio Frequency Quadrupole (RFQ) accelerator and IH-DTL linac. It is also used for measuring a kinetic energy of ion beam by time-of-flight (TOF) method using two phase probes. The capacitive beam phase probe has been developed. The electromagnetic design of the high precision phase probe was performed to satisfy the phase resolution of 1° (@200 MHz). It was confirmed by the test result using a wire test bench. The measured phase accuracy of the fabricated phase probe is 1.19 ps. The pre-amplifier electronics with the 0.125 ∼ 1.61 GHz broad-band was designed and fabricated for amplifying the signal strength. The results of RF frequency and beam energy measurement using a proton beam from the cyclotron in KIRAMS is presented.

  9. IVVS probe mechanical concept design

    Energy Technology Data Exchange (ETDEWEB)

    Rossi, Paolo, E-mail: paolo.rossi@enea.it; Neri, Carlo; De Collibus, Mario Ferri; Mugnaini, Giampiero; Pollastrone, Fabio; Crescenzi, Fabio

    2015-10-15

    Highlights: • ENEA designed, developed and tested a laser based In Vessel Viewing System (IVVS). • IVVS mechanical design has been revised from 2011 to 2013 to meet ITER requirements. • Main improvements are piezoceramic actuators and a step focus system. • Successful qualification activities validated the concept design for ITER environment. - Abstract: ENEA has been deeply involved in the design, development and testing of a laser based In Vessel Viewing System (IVVS) required for the inspection of ITER plasma-facing components. The IVVS probe shall be deployed into the vacuum vessel, providing high resolution images and metrology measurements to detect damages and possible erosion. ENEA already designed and manufactured an IVVS probe prototype based on a rad-hard concept and driven by commercial micro-step motors, which demonstrated satisfying viewing and metrology performances at room conditions. The probe sends a laser beam through a reflective rotating prism. By rotating the axes of the prism, the probe can scan all the environment points except those present in a shadow cone and the backscattered light signal is then processed to measure the intensity level (viewing) and the distance from the probe (metrology). During the last years, in order to meet all the ITER environmental conditions, such as high vacuum, gamma radiation lifetime dose up to 5 MGy, cumulative neutron fluence of about 2.3 × 10{sup 17} n/cm{sup 2}, temperature of 120 °C and magnetic field of 8 T, the probe mechanical design was significantly revised introducing a new actuating system based on piezo-ceramic actuators and improved with a new step focus system. The optical and mechanical schemes have been then modified and refined to meet also the geometrical constraints. The paper describes the mechanical concept design solutions adopted in order to fulfill IVVS probe functional performance requirements considering ITER working environment and geometrical constraints.

  10. Polarization of submillimetre lines from interstellar medium

    Science.gov (United States)

    Zhang, Heshou; Yan, Huirong

    2018-04-01

    Magnetic fields play important roles in many astrophysical processes. However, there is no universal diagnostic for the magnetic fields in the interstellar medium (ISM) and each magnetic tracer has its limitation. Any new detection method is thus valuable. Theoretical studies have shown that submillimetre fine-structure lines are polarized due to atomic alignment by ultraviolet photon-excitation, which opens up a new avenue to probe interstellar magnetic fields. We will, for the first time, perform synthetic observations on the simulated three-dimensional ISM to demonstrate the measurability of the polarization of submillimetre atomic lines. The maximum polarization for different absorption and emission lines expected from various sources, including star-forming regions are provided. Our results demonstrate that the polarization of submillimetre atomic lines is a powerful magnetic tracer and add great value to the observational studies of the submilimetre astronomy.

  11. On a laser beam fiducial line application for metrological purposes

    International Nuclear Information System (INIS)

    Batusov, V.; Budagov, J.; Lyablin, M.; Rusakovich, N.; Sisakyan, A.; Topilin, N.; Khubua, J.; Lasseur, C.

    2008-01-01

    The possibility of a collimated one-mode laser beam used as a fiducial line is considered. The technology of an 'extended' laser beam formation and application for a much extended fiducial line is proposed

  12. A prototype methodology combining surface-enhanced laser desorption/ionization protein chip technology and artificial neural network algorithms to predict the chemoresponsiveness of breast cancer cell lines exposed to Paclitaxel and Doxorubicin under in vitro conditions.

    Science.gov (United States)

    Mian, Shahid; Ball, Graham; Hornbuckle, Jo; Holding, Finn; Carmichael, James; Ellis, Ian; Ali, Selman; Li, Geng; McArdle, Stephanie; Creaser, Colin; Rees, Robert

    2003-09-01

    An ability to predict the likelihood of cellular response towards particular chemotherapeutic agents based upon protein expression patterns could facilitate the identification of biological molecules with previously undefined roles in the process of chemoresistance/chemosensitivity, and if robust enough these patterns might also be exploited towards the development of novel predictive assays. To ascertain whether proteomic based molecular profiling in conjunction with artificial neural network (ANN) algorithms could be applied towards the specific recognition of phenotypic patterns between either control or drug treated and chemosensitive or chemoresistant cellular populations, a combined approach involving MALDI-TOF matrix-assisted laser desorption/ionization-time of flight mass spectrometry, Ciphergen protein chip technology and ANN algorithms have been applied to specifically identify proteomic 'fingerprints' indicative of treatment regimen for chemosensitive (MCF-7, T47D) and chemoresistant (MCF-7/ADR) breast cancer cell lines following exposure to Doxorubicin or Paclitaxel. The results indicate that proteomic patterns can be identified by ANN algorithms to correctly assign 'class' for treatment regimen (e.g. control/drug treated or chemosensitive/chemoresistant) with a high degree of accuracy using boot-strap statistical validation techniques and that biomarker ion patterns indicative of response/non-response phenotypes are associated with MCF-7 and MCF-7/ADR cells exposed to Doxorubicin. We have also examined the predictive capability of this approach towards MCF-7 and T47D cells to ascertain whether prediction could be made based upon treatment regimen irrespective of cell lineage. Models were identified that could correctly assign class (control or Paclitaxel treatment) for 35/38 samples of an independent dataset. A similar level of predictive capability was also found (> 92%; n = 28) when proteomic patterns derived from the drug resistant cell line MCF-7

  13. Multimode electromagnetically induced transparency on a single atomic line

    International Nuclear Information System (INIS)

    Campbell, Geoff; Ordog, Anna; Lvovsky, A I

    2009-01-01

    We experimentally investigate electromagnetically induced transparency (EIT) created on an inhomogeneously broadened 5S 1/2 -5P 1/2 transition in rubidium vapor using a control field of a complex temporal shape. A comb-shaped transparency spectrum enhances the delay-bandwidth product and the light storage capacity for a matched probe pulse by a factor of about 50 compared to a single EIT line (Yavuz 2007 Phys. Rev. A 75 031801). If the temporal mode of the control field is slowly changed while the probe is propagating through the EIT medium, the probe will adiabatically follow, providing a means to perform frequency conversion and optical routing.

  14. Eddy-current probe design

    International Nuclear Information System (INIS)

    Kincaid, T.G.; McCary, R.O.

    1983-01-01

    This paper describes theoretical and experimental work directed toward finding the optimum probe dimensions and operating frequency for eddy current detection of half-penny surface cracks in nonmagnetic conducting materials. The study applies to probes which excite an approximately uniform spatial field over the length of the crack at the surface of the material. In practical terms, this means that the probe is not smaller than the crack length in any of its critical dimensions. The optimization of a simple coil probe is first analyzed in detail. It is shown that signal-to-noise ratio and lift-off discrimination are maximized by a pancake coil with mean radius not greater than the crack length, operated at a frequency which gives a skin depth equal to the crack depth. The results obtained for the simple coil are then used as a basis for discussion of the design of coils with ferrite cores and shields, and for the design of recording head type probes

  15. Autoionization spectral line shapes in dense plasmas

    International Nuclear Information System (INIS)

    Rosmej, F.B.; Hoffmann, D.H.H.; Faenov, A.Ya.; Pikuz, T.A.; Suess, W.; Geissel, M.

    2001-01-01

    The distortion of resonance line shapes due to the accumulation of a large number of satellite transitions is discovered by means of X-ray optical methods with simultaneous high spectral (λ/δλ≅8000) and spatial resolution (δx≅7 μm). Disappearance of the He α resonance line emission near the target surface is observed while Rydberg satellite intensity accumulates near the resonance line position. He β and He γ resonance line shapes are also shown to be seriously affected by opacity, higher-order line emissions from autoionizing states and inhomogeneous spatial emission. Opposite to resonance line emissions the He β satellites originate only from a very narrow spatial interval. New temperature and density diagnostics employing the 1s2131' and 1s3131'-satellites are developed. Moreover, even-J components of the satellite line emissions were resolved in the present high resolution experiments. Line transitions from the autoionizing states 1s2131' are therefore also proposed for space resolved Stark broadening analysis and local high density probing. Theorists are encouraged to provide accurate Stark broadening data for the transitions 1s2131 ' →1s 2 21+hv

  16. Transmission Line Security Monitor: Final Report

    Energy Technology Data Exchange (ETDEWEB)

    John Svoboda

    2011-04-01

    The Electric Power Transmission Line Security Monitor System Operational Test is a project funded by the Technical Support Working Group (TSWG). TSWG operates under the Combating Terrorism Technical Support Office that functions under the Department of Defense. The Transmission Line Security Monitor System is based on technology developed by Idaho National Laboratory. The technology provides a means for real-time monitoring of physical threats and/or damage to electrical transmission line towers and conductors as well as providing operational parameters to transmission line operators to optimize transmission line operation. The end use is for monitoring long stretches of transmission lines that deliver electrical power from remote generating stations to cities and industry. These transmission lines are generally located in remote transmission line corridors where security infrastructure may not exist. Security and operational sensors in the sensor platform on the conductors take power from the transmission line and relay security and operational information to operations personnel hundreds of miles away without relying on existing infrastructure. Initiated on May 25, 2007, this project resulted in pre-production units tested in realistic operational environments during 2010. A technology licensee, Lindsey Manufacturing of Azusa California, is assisting in design, testing, and ultimately production. The platform was originally designed for a security monitoring mission, but it has been enhanced to include important operational features desired by electrical utilities.

  17. Ultra-thin flexible polyimide neural probe embedded in a dissolvable maltose-coated microneedle

    International Nuclear Information System (INIS)

    Xiang, Zhuolin; Yen, Shih-Cheng; Zhang, Songsong; Lee, Chengkuo; Xue, Ning; Sun, Tao; Tsang, Wei Mong; Liao, Lun-De; Thakor, Nitish V

    2014-01-01

    The ultra-thin flexible polyimide neural probe can reduce the glial sheath growth on the probe body while its flexibility can minimize the micromotion between the probe and brain tissue. To provide sufficient stiffness for penetration purposes, we developed a drawing lithography technology for uniform maltose coating to make the maltose-coated polyimide neural probe become a stiff microneedle. The coating thicknesses under different temperature and the corresponding stiffness are studied. It has been proven that the coated maltose is dissolved by body fluids after implantation for a few seconds. Moreover, carbon nanotubes are coated on the neural probe recording electrodes to improve the charge delivery ability and reduce the impedance. Last but not least, the feasibility and recording characteristic of this ultra-thin polyimide neural probe embedded in a maltose-coated microneedle are further demonstrated by in vivo tests. (paper)

  18. Ultra-thin flexible polyimide neural probe embedded in a dissolvable maltose-coated microneedle

    Science.gov (United States)

    Xiang, Zhuolin; Yen, Shih-Cheng; Xue, Ning; Sun, Tao; Mong Tsang, Wei; Zhang, Songsong; Liao, Lun-De; Thakor, Nitish V.; Lee, Chengkuo

    2014-06-01

    The ultra-thin flexible polyimide neural probe can reduce the glial sheath growth on the probe body while its flexibility can minimize the micromotion between the probe and brain tissue. To provide sufficient stiffness for penetration purposes, we developed a drawing lithography technology for uniform maltose coating to make the maltose-coated polyimide neural probe become a stiff microneedle. The coating thicknesses under different temperature and the corresponding stiffness are studied. It has been proven that the coated maltose is dissolved by body fluids after implantation for a few seconds. Moreover, carbon nanotubes are coated on the neural probe recording electrodes to improve the charge delivery ability and reduce the impedance. Last but not least, the feasibility and recording characteristic of this ultra-thin polyimide neural probe embedded in a maltose-coated microneedle are further demonstrated by in vivo tests.

  19. Ultra-shallow junction (USJ) sheet resistance measurements with a non-penetrating four point probe

    International Nuclear Information System (INIS)

    Benjamin, M.C.; Hillard, R.J.; Borland, J.O.

    2005-01-01

    An accurate method to measure the four point probe (4PP) sheet resistance (R S ) of ultra shallow junction (USJ) Source-Drain Extension structures is described. The method utilizes Elastic Material probes (EM-probes) to form non-penetrating contacts to the silicon surface [R.J. Hillard, P.Y. Hung, William Chism, C. Win Ye, W.H. Howland, L.C. Tan, C.E. Kalnas, Characterization and Metrology for ULSI Technology, AIP Conference proceedings 683 (2003) 802.]. The probe design is kinematic and the force is controlled to ensure elastic deformation of the probe material. The probe material is such that large direct tunneling currents can flow through the native oxide thereby forming a low impedance contact. Sheet resistance measurements on USJ implanted P+/N structures with Secondary Ion Mass Spectroscopy (SIMS) junction depths less than 15 nm have been measured. The method is demonstrated on implanted USJ structures and found to be consistent with expectations

  20. Theoretical investigation of field-line quality in a driven spheromak

    International Nuclear Information System (INIS)

    Cohen, R.H.; Cohen, B.I.; Berk, H.L.

    2003-01-01

    Theoretical studies aimed at predicting and diagnosing field-line quality in a spheromak are described. These include nonlinear 3-D MHD simulations, stability studies, analyses of confinement in spheromaks dominated by either open (stochastic) field lines or approximate flux surfaces, and a theory of fast electrons as a probe of field-line length. (author)

  1. Identification of MgII Absorption Line Systems from SDSS Quasar ...

    Indian Academy of Sciences (India)

    Motivation. The quasar absorption lines are crucial to our understanding of the Universe since the absorption lines provide a wealth of information on the gaseous Universe from high redshift to present day. The absorption lines can also allow us to probe the metallicity and ionization state of the gas (Wild et al. 2008).

  2. Gamma-ray imaging probes

    International Nuclear Information System (INIS)

    Wild, W.J.

    1988-01-01

    External nuclear medicine diagnostic imaging of early primary and metastatic lung cancer tumors is difficult due to the poor sensitivity and resolution of existing gamma cameras. Nonimaging counting detectors used for internal tumor detection give ambiguous results because distant background variations are difficult to discriminate from neighboring tumor sites. This suggests that an internal imaging nuclear medicine probe, particularly an esophageal probe, may be advantageously used to detect small tumors because of the ability to discriminate against background variations and the capability to get close to sites neighboring the esophagus. The design, theory of operation, preliminary bench tests, characterization of noise behavior and optimization of such an imaging probe is the central theme of this work

  3. Scanning vector Hall probe microscopy

    International Nuclear Information System (INIS)

    Cambel, V.; Gregusova, D.; Fedor, J.; Kudela, R.; Bending, S.J.

    2004-01-01

    We have developed a scanning vector Hall probe microscope for mapping magnetic field vector over magnetic samples. The microscope is based on a micromachined Hall sensor and the cryostat with scanning system. The vector Hall sensor active area is ∼5x5 μm 2 . It is realized by patterning three Hall probes on the tilted faces of GaAs pyramids. Data from these 'tilted' Hall probes are used to reconstruct the full magnetic field vector. The scanning area of the microscope is 5x5 mm 2 , space resolution 2.5 μm, field resolution ∼1 μT Hz -1/2 at temperatures 10-300 K

  4. Spaser as a biological probe

    Science.gov (United States)

    Galanzha, Ekaterina I.; Weingold, Robert; Nedosekin, Dmitry A.; Sarimollaoglu, Mustafa; Nolan, Jacqueline; Harrington, Walter; Kuchyanov, Alexander S.; Parkhomenko, Roman G.; Watanabe, Fumiya; Nima, Zeid; Biris, Alexandru S.; Plekhanov, Alexander I.; Stockman, Mark I.; Zharov, Vladimir P.

    2017-06-01

    Understanding cell biology greatly benefits from the development of advanced diagnostic probes. Here we introduce a 22-nm spaser (plasmonic nanolaser) with the ability to serve as a super-bright, water-soluble, biocompatible probe capable of generating stimulated emission directly inside living cells and animal tissues. We have demonstrated a lasing regime associated with the formation of a dynamic vapour nanobubble around the spaser that leads to giant spasing with emission intensity and spectral width >100 times brighter and 30-fold narrower, respectively, than for quantum dots. The absorption losses in the spaser enhance its multifunctionality, allowing for nanobubble-amplified photothermal and photoacoustic imaging and therapy. Furthermore, the silica spaser surface has been covalently functionalized with folic acid for molecular targeting of cancer cells. All these properties make a nanobubble spaser a promising multimodal, super-contrast, ultrafast cellular probe with a single-pulse nanosecond excitation for a variety of in vitro and in vivo biomedical applications.

  5. Fluorescent peptide biosensor for probing the relative abundance of cyclin-dependent kinases in living cells.

    Directory of Open Access Journals (Sweden)

    Laetitia Kurzawa

    Full Text Available Cyclin-dependant kinases play a central role in coordinating cell growth and division, and in sustaining proliferation of cancer cells, thereby constituting attractive pharmacological targets. However, there are no direct means of assessing their relative abundance in living cells, current approaches being limited to antigenic and proteomic analysis of fixed cells. In order to probe the relative abundance of these kinases directly in living cells, we have developed a fluorescent peptide biosensor with biligand affinity for CDKs and cyclins in vitro, that retains endogenous CDK/cyclin complexes from cell extracts, and that bears an environmentally-sensitive probe, whose fluorescence increases in a sensitive fashion upon recognition of its targets. CDKSENS was introduced into living cells, through complexation with the cell-penetrating carrier CADY2 and applied to assess the relative abundance of CDK/Cyclins through fluorescence imaging and ratiometric quantification. This peptide biosensor technology affords direct and sensitive readout of CDK/cyclin complex levels, and reports on differences in complex formation when tampering with a single CDK or cyclin. CDKSENS further allows for detection of differences between different healthy and cancer cell lines, thereby enabling to distinguish cells that express high levels of these heterodimeric kinases, from cells that present decreased or defective assemblies. This fluorescent biosensor technology provides information on the overall status of CDK/Cyclin complexes which cannot be obtained through antigenic detection of individual subunits, in a non-invasive fashion which does not require cell fixation or extraction procedures. As such it provides promising perspectives for monitoring the response to therapeutics that affect CDK/Cyclin abundance, for cell-based drug discovery strategies and fluorescence-based cancer diagnostics.

  6. Invited Review Article: Pump-probe microscopy

    Science.gov (United States)

    Wilson, Jesse W.; Robles, Francisco E.; Warren, Warren S.

    2016-01-01

    Multiphoton microscopy has rapidly gained popularity in biomedical imaging and materials science because of its ability to provide three-dimensional images at high spatial and temporal resolution even in optically scattering environments. Currently the majority of commercial and home-built devices are based on two-photon fluorescence and harmonic generation contrast. These two contrast mechanisms are relatively easy to measure but can access only a limited range of endogenous targets. Recent developments in fast laser pulse generation, pulse shaping, and detection technology have made accessible a wide range of optical contrasts that utilize multiple pulses of different colors. Molecular excitation with multiple pulses offers a large number of adjustable parameters. For example, in two-pulse pump-probe microscopy, one can vary the wavelength of each excitation pulse, the detection wavelength, the timing between the excitation pulses, and the detection gating window after excitation. Such a large parameter space can provide much greater molecular specificity than existing single-color techniques and allow for structural and functional imaging without the need for exogenous dyes and labels, which might interfere with the system under study. In this review, we provide a tutorial overview, covering principles of pump-probe microscopy and experimental setup, challenges associated with signal detection and data processing, and an overview of applications. PMID:27036751

  7. Invited Review Article: Pump-probe microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Fischer, Martin C., E-mail: Martin.Fischer@duke.edu; Wilson, Jesse W.; Robles, Francisco E. [Department of Chemistry, Duke University, Durham, North Carolina 27708 (United States); Warren, Warren S. [Departments of Chemistry, Biomedical Engineering, Physics, and Radiology, Duke University, Durham, North Carolina 27708 (United States)

    2016-03-15

    Multiphoton microscopy has rapidly gained popularity in biomedical imaging and materials science because of its ability to provide three-dimensional images at high spatial and temporal resolution even in optically scattering environments. Currently the majority of commercial and home-built devices are based on two-photon fluorescence and harmonic generation contrast. These two contrast mechanisms are relatively easy to measure but can access only a limited range of endogenous targets. Recent developments in fast laser pulse generation, pulse shaping, and detection technology have made accessible a wide range of optical contrasts that utilize multiple pulses of different colors. Molecular excitation with multiple pulses offers a large number of adjustable parameters. For example, in two-pulse pump-probe microscopy, one can vary the wavelength of each excitation pulse, the detection wavelength, the timing between the excitation pulses, and the detection gating window after excitation. Such a large parameter space can provide much greater molecular specificity than existing single-color techniques and allow for structural and functional imaging without the need for exogenous dyes and labels, which might interfere with the system under study. In this review, we provide a tutorial overview, covering principles of pump-probe microscopy and experimental setup, challenges associated with signal detection and data processing, and an overview of applications.

  8. DNA Probe for Lactobacillus delbrueckii

    Science.gov (United States)

    Delley, Michèle; Mollet, Beat; Hottinger, Herbert

    1990-01-01

    From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific DNA probe. Dot-blot hybridizations with over 40 different Lactobacillus strains showed that this clone specifically recognizes L. delbrueckii subsp. delbrueckii, bulgaricus, and lactis. The sensitivity of the method was tested by using an α-32P-labeled DNA probe. Images PMID:16348233

  9. Radical probing of spliceosome assembly.

    Science.gov (United States)

    Grewal, Charnpal S; Kent, Oliver A; MacMillan, Andrew M

    2017-08-01

    Here we describe the synthesis and use of a directed hydroxyl radical probe, tethered to a pre-mRNA substrate, to map the structure of this substrate during the spliceosome assembly process. These studies indicate an early organization and proximation of conserved pre-mRNA sequences during spliceosome assembly. This methodology may be adapted to the synthesis of a wide variety of modified RNAs for use as probes of RNA structure and RNA-protein interaction. Copyright © 2017 Elsevier Inc. All rights reserved.

  10. Architectural Probes of the Infraordinary

    DEFF Research Database (Denmark)

    Lunde Nielsen, Espen

    2017-01-01

    of the city plays a vital role for the social coexistence of and the correlation between its inhabitants. In an era of explosive growth of our cities, it is crucial to critically examine the everyday social dimension, if our cities are to be liveable in the future. To enquire into the everyday topography...... approaches for probing into and interrogating the infraordinary: frameworks of perception and situated probes. Both are deployed in order to get at distance of the familiar and by-pass the usual hierarchies of perception to gain new knowledge. These critical spatial practices span an interdisciplinary...

  11. Detecting device of atomic probe

    International Nuclear Information System (INIS)

    Nikonenkov, N.V.

    1979-01-01

    Operation of an atomic-probe recording device is discussed in detail and its flowsheet is given. The basic elements of the atomic-probe recording device intented for microanalysis of metals and alloys in an atomic level are the storage oscillograph with a raster-sweep unit, a two-channel timer using frequency meters, a digital printer, and a control unit. The digital printer records information supplied by four digital devices (two frequency meters and two digital voltmeters) in a four-digit binary-decimal code. The described device provides simultaneous recording of two ions produced per one vaporation event

  12. Probing nuclear matter with dileptons

    International Nuclear Information System (INIS)

    Schroeder, L.S.

    1986-06-01

    Dileptons are shown to be of interest in helping probe extreme conditions of temperature and density in nuclear matter. The current state of experimental knowledge about dileptons is briefly described, and their use in upcoming experiments with light ions at CERN SPS are reviewed, including possible signatures of quark matter formation. Use of dileptons in an upcoming experiment with a new spectrometer at Berkeley is also discussed. This experiment will probe the nuclear matter equation of state at high temperature and density. 16 refs., 8 figs

  13. Radioactive Probes on Ferromagnetic Surfaces

    CERN Multimedia

    2002-01-01

    On the (broad) basis of our studies of nonmagnetic radioactive probe atoms on magnetic surfaces and at interfaces, we propose to investigate the magnetic interaction of magnetic probe atoms with their immediate environment, in particular of rare earth (RE) elements positioned on and in ferromagnetic surfaces. The preparation and analysis of the structural properties of such samples will be performed in the UHV chamber HYDRA at the HMI/Berlin. For the investigations of the magnetic properties of RE atoms on surfaces Perturbed Angular Correlation (PAC) measurements and Mössbauer Spectroscopy (MS) in the UHV chamber ASPIC (Apparatus for Surface Physics and Interfaces at CERN) are proposed.

  14. Free-radical probes for functional in vivo EPR imaging

    Science.gov (United States)

    Subramanian, S.; Krishna, M. C.

    2007-02-01

    Electron paramagnetic resonance imaging (EPRI) is one of the recent functional imaging modalities that can provide valuable in vivo physiological information on its own merit and aids as a complimentary imaging technique to MRI and PET of tissues especially with respect to in vivo pO II (oxygen partial pressure), redox status and pharmacology. EPR imaging mainly deals with the measurement of distribution and in vivo dynamics and redox changes using special nontoxic paramagnetic spin probes that can be infused into the object of investigation. These spin probes should be characterized by simple EPR spectra, preferably with narrow EPR lines. The line width should be reversibly sensitive to the concentration of in vivo pO II with a linear dependence. Several non-toxic paramagnetic probes, some particulate and insoluble and others water-soluble and infusible (by intravenous or intramuscular injection) have been developed which can be effectively used to quantitatively assess tissue redox status, and tumor hypoxia. Quantitative assessment of the redox status of tissue in vivo is important in investigating oxidative stress, and that of tissue pO II is very important in radiation oncology. Other areas in which EPR imaging and oxymetry may help are in the investigation of tumorangiogenesis, wound healing, oxygenation of tumor tissue by the ingestion of oxygen-rich gases, etc. The correct choice of the spin probe will depend on the modality of measurement (whether by CW or time-domain EPR imaging) and the particular physiology interrogated. Examples of the available spin probes and some EPR imaging applications employing them are presented.

  15. Refractory Coated/Lined Low Density Structures, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — This project addresses the development of refractory coated or lined low density structures applicable for advanced future propulsion system technologies. The...

  16. Human Leukocyte Antigen Typing Using a Knowledge Base Coupled with a High-Throughput Oligonucleotide Probe Array Analysis

    Science.gov (United States)

    Zhang, Guang Lan; Keskin, Derin B.; Lin, Hsin-Nan; Lin, Hong Huang; DeLuca, David S.; Leppanen, Scott; Milford, Edgar L.; Reinherz, Ellis L.; Brusic, Vladimir

    2014-01-01

    Human leukocyte antigens (HLA) are important biomarkers because multiple diseases, drug toxicity, and vaccine responses reveal strong HLA associations. Current clinical HLA typing is an elimination process requiring serial testing. We present an alternative in situ synthesized DNA-based microarray method that contains hundreds of thousands of probes representing a complete overlapping set covering 1,610 clinically relevant HLA class I alleles accompanied by computational tools for assigning HLA type to 4-digit resolution. Our proof-of-concept experiment included 21 blood samples, 18 cell lines, and multiple controls. The method is accurate, robust, and amenable to automation. Typing errors were restricted to homozygous samples or those with very closely related alleles from the same locus, but readily resolved by targeted DNA sequencing validation of flagged samples. High-throughput HLA typing technologies that are effective, yet inexpensive, can be used to analyze the world’s populations, benefiting both global public health and personalized health care. PMID:25505899

  17. Probe suppression in conformal phased array

    CERN Document Server

    Singh, Hema; Neethu, P S

    2017-01-01

    This book considers a cylindrical phased array with microstrip patch antenna elements and half-wavelength dipole antenna elements. The effect of platform and mutual coupling effect is included in the analysis. The non-planar geometry is tackled by using Euler's transformation towards the calculation of array manifold. Results are presented for both conducting and dielectric cylinder. The optimal weights obtained are used to generate adapted pattern according to a given signal scenario. It is shown that array along with adaptive algorithm is able to cater to an arbitrary signal environment even when the platform effect and mutual coupling is taken into account. This book provides a step-by-step approach for analyzing the probe suppression in non-planar geometry. Its detailed illustrations and analysis will be a useful text for graduate and research students, scientists and engineers working in the area of phased arrays, low-observables and stealth technology.

  18. A heat source probe for measuring thermal conductivity in waste rock dumps

    International Nuclear Information System (INIS)

    Blackford, M.G.; Harries, J.R.

    1985-10-01

    The development and use of a heat source probe to measure the thermal conductivity of the material in a waste rock dump is described. The probe releases heat at a constant rate into the surrounding material and the resulting temperature rise is inversely related to the thermal conductivity. The probe was designed for use in holes in the dump which are lined with 50 mm i.d. polyethylene liners. The poor thermal contact between the probe and the liner and the unknown conductivity of the backfill material around the liner necessitated long heating and cooling times (>10 hours) to ensure that the thermal conductivity of the dump material was being measured. Temperature data acquired in the field were analysed by comparing them with temperatures calculated using a two-dimensional cylindrical model of the probe and surrounding material, and the heat transfer code HEATRAN

  19. Development of a novel gamma probe for detecting radiation direction

    Science.gov (United States)

    Pani, R.; Pellegrini, R.; Cinti, M. N.; Longo, M.; Donnarumma, R.; D'Alessio, A.; Borrazzo, C.; Pergola, A.; Ridolfi, S.; De Vincentis, G.

    2016-01-01

    Spatial localization of radioactive sources is currently a main issue interesting different fields, including nuclear industry, homeland security as well as medical imaging. It is currently achieved using different systems, but the development of technologies for detecting and characterizing radiation is becoming important especially in medical imaging. In this latter field, radiation detection probes have long been used to guide surgery, thanks to their ability to localize and quantify radiopharmaceutical uptake even deep in tissue. Radiolabelled colloid is injected into, or near to, the tumor and the surgeon uses a hand-held radiation detector, the gamma probe, to identify lymph nodes with radiopharmaceutical uptkake. The present work refers to a novel scintigraphic goniometric probe to identify gamma radiation and its direction. The probe incorporates several scintillation crystals joined together in a particular configuration to provide data related to the position of a gamma source. The main technical characteristics of the gamma locator prototype, i.e. sensitivity, spatial resolution and detection efficiency, are investigated. Moreover, the development of a specific procedure applied to the images permits to retrieve the source position with high precision with respect to the currently used gamma probes. The presented device shows a high sensitivity and efficiency to identify gamma radiation taking a short time (from 30 to 60 s). Even though it was designed for applications in radio-guided surgery, it could be used for other purposes, as for example homeland security.

  20. Development of a novel gamma probe for detecting radiation direction

    International Nuclear Information System (INIS)

    Pani, R.; Pellegrini, R.; Cinti, M.N.; Longo, M.; Donnarumma, R.; Borrazzo, C.; D'Alessio, A.; Pergola, A.; Ridolfi, S.; Vincentis, G. De

    2016-01-01

    Spatial localization of radioactive sources is currently a main issue interesting different fields, including nuclear industry, homeland security as well as medical imaging. It is currently achieved using different systems, but the development of technologies for detecting and characterizing radiation is becoming important especially in medical imaging. In this latter field, radiation detection probes have long been used to guide surgery, thanks to their ability to localize and quantify radiopharmaceutical uptake even deep in tissue. Radiolabelled colloid is injected into, or near to, the tumor and the surgeon uses a hand-held radiation detector, the gamma probe, to identify lymph nodes with radiopharmaceutical uptkake. The present work refers to a novel scintigraphic goniometric probe to identify gamma radiation and its direction. The probe incorporates several scintillation crystals joined together in a particular configuration to provide data related to the position of a gamma source. The main technical characteristics of the gamma locator prototype, i.e. sensitivity, spatial resolution and detection efficiency, are investigated. Moreover, the development of a specific procedure applied to the images permits to retrieve the source position with high precision with respect to the currently used gamma probes. The presented device shows a high sensitivity and efficiency to identify gamma radiation taking a short time (from 30 to 60 s). Even though it was designed for applications in radio-guided surgery, it could be used for other purposes, as for example homeland security

  1. Possible concepts for an in situ Saturn probe mission

    Science.gov (United States)

    Coustenis, Athena; Lebreton, Jean-Pierre; Mousis, Olivier; Atkinson, David H.; Lunine, Jonathan I.; Reh, Kim R.; Fletcher, Leigh N.; Simon-Miller, Amy A.; Atreya, Sushil; Brinckerhoff, William B.; Cavalie, Thibault; Colaprete, Anthony; Gautier, Daniel; Guillot, Tristan; Mahaffy, Paul R.; Marty, Bernard; Morse, Andy; Sims, Jon; Spilker, Tom; Spilker, Linda

    2014-05-01

    carrier several months prior to probe entry. The carrier trajectory would be designed to enable probe data relay during over-flight as well as performing approach and flyby science; • Configuration 3: Probe + Orbiter (similar to the Galileo Orbiter/Probe). As for Configuration 2, but after probe relay during over-flight, the orbiter would transition to a Saturn orbit and continue to perform orbital science. In all three configurations, the carrier/orbiter would be equipped with a combination of solar panels, secondary batteries and possibly a set of primary batteries for phases that require a high power demand, for example during the probe entry phase. Nuclear power would be considered for the carrier or the orbiter only if available solar power technology would be found to be infeasible. To match the measurement requirements, a model payload could include a mass spectrometer, a tunable laser system, a helium abundance detector, an atmospheric structure instrument, accelerometers, temperature sensors, pressure profile, Doppler wind and nephelometer instruments, etc. Such a mission would greatly benefit from strong international collaborations. References [1] Mousis et al. 2014, "Scientific Rationale of Saturn's in situ exploration", submitted to PSS (and references therein).

  2. Characterization of near-field optical probes

    DEFF Research Database (Denmark)

    Vohnsen, Brian; Bozhevolnyi, Sergey I.

    1999-01-01

    Radiation and collection characteristics of four different near-field optical-fiber probes, namely, three uncoated probes and an aluminium-coated small-aperture probe, are investigated and compared. Their radiation properties are characterized by observation of light-induced topography changes...... in a photo-sensitive film illuminated with the probes, and it is confirmed that the radiated optical field is unambigiously confined only for the coated probe. Near-field optical imaging of a standing evanescent-wave pattern is used to compare the detection characteristics of the probes, and it is concluded...... that, for the imaging of optical-field intensity distributions containing predominantly evanescent-wave components, a sharp uncoated tip is the probe of choice. Complementary results obtained with optical phase-conjugation experiments with he uncoated probes are discussed in relation to the probe...

  3. The Gravity Probe B Flight Dewar

    Science.gov (United States)

    2001-01-01

    Gravity Probe B (GP-B) is the relativity experiment developed at Stanford University to test two extraordinary predictions of Albert Einstein's general theory of relativity. This photograph is of the Gravity Probe B flight dewar, a metal container made like a vacuum bottle that is used especially for storing liquefied gases, that will maintain the experiment at a temperature just above absolute zero, staying cold for two years. The experiment will measure, very precisely, the expected tiny changes in the direction of the spin axes of four gyroscopes contained in an Earth-orbiting satellite at 400-mile altitude. So free are the gyroscopes from disturbance that they will provide an almost perfect space-time reference system. They will measure how space and time are very slightly warped by the presence of the Earth, and, more profoundly, how the Earth's rotation very slightly drags space-time around with it. These effects, though small for the Earth, have far-reaching implications for the nature of matter and the structure of the Universe. GP-B is among the most thoroughly researched programs ever undertaken by NASA. This is the story of a scientific quest in which physicists and engineers have collaborated closely over many years. Inspired by their quest, they have invented a whole range of technologies -- technologies that are already enlivening other branches of science and engineering. Launched in 2004 and managed for NASA by the Marshall Space Flight Center, development of the GP-B is the responsibility of Stanford University, with major subcontractor Lockheed Martin Corporation. (Photo Credit: Lockheed Martin Corporation/R. Underwood)

  4. Cable line engineering

    International Nuclear Information System (INIS)

    Jang, Hak Sin; Kim, Sin Yeong

    1998-02-01

    This book is about cable line engineering. It is comprised of nine chapters, which deals with summary of cable communication such as way, process of cable communication and optical communication, Line constant of transmission on primary constant, reflection and crosstalk, communication cable line of types like flat cable, coaxial cable and loaded cable, Install of communication line with types and facility of aerial line, construction method of communication line facility, Measurement of communication line, Carrier communication of summary, PCM communication with Introduction, regeneration relay system sampling and quantization and Electric communication service and general information network with mobile communication technique and satellite communication system.

  5. Nuclear physics with electroweak probes

    International Nuclear Information System (INIS)

    Benhar, Omar

    2009-01-01

    In recent years, the italian theoretical Nuclear Physics community has played a leading role in the development of a unified approach, allowing for a consistent and fully quantitative description of the nuclear response to electromagnetic and weak probes. In this paper I review the main achievements in both fields, point out some of the open problems, and outline the most promising prospects

  6. Resolution analysis by random probing

    NARCIS (Netherlands)

    Fichtner, Andreas; van Leeuwen, T.

    2015-01-01

    We develop and apply methods for resolution analysis in tomography, based on stochastic probing of the Hessian or resolution operators. Key properties of our methods are (i) low algorithmic complexity and easy implementation, (ii) applicability to any tomographic technique, including full‐waveform

  7. A fluorescent probe for ecstasy.

    Science.gov (United States)

    Masseroni, D; Biavardi, E; Genovese, D; Rampazzo, E; Prodi, L; Dalcanale, E

    2015-08-18

    A nanostructure formed by the insertion in silica nanoparticles of a pyrene-derivatized cavitand, which is able to specifically recognize ecstasy in water, is presented. The absence of effects from interferents and an efficient electron transfer process occurring after complexation of ecstasy, makes this system an efficient fluorescent probe for this popular drug.

  8. Probing Pharmaceutical Mixtures during Milling

    DEFF Research Database (Denmark)

    Walker, Greg; Römann, Philipp; Poller, Bettina

    2017-01-01

    interpret the spectral changes. Overall, this study demonstrates the potential of low-frequency Raman spectroscopy, which has several practical advantages over XRPD, for probing (dis-)order during pharmaceutical processing, showcasing its potential for future development, and implementation as an in...

  9. A Product Line Enhanced Unified Process

    DEFF Research Database (Denmark)

    Zhang, Weishan; Kunz, Thomas

    2006-01-01

    The Unified Process facilitates reuse for a single system, but falls short handling multiple similar products. In this paper we present an enhanced Unified Process, called UPEPL, integrating the product line technology in order to alleviate this problem. In UPEPL, the product line related activit...... activities are added and could be conducted side by side with other classical UP activities. In this way both the advantages of Unified Process and software product lines could co-exist in UPEPL. We show how to use UPEPL with an industrial mobile device product line in our case study....

  10. Advantages of the first-derivative probe technique over the three- and four-parameter probe techniques in fusion plasmas diagnostics

    Czech Academy of Sciences Publication Activity Database

    Hasan, E.; Dimitrova, Miglena; Popov, T.; Ivanova, P.; Dejarnac, Renaud; Stöckel, Jan; Pánek, Radomír

    2018-01-01

    Roč. 13, č. 4 (2018), č. článku P04005. ISSN 1748-0221 R&D Projects: GA MŠk(CZ) LM2015045 Institutional support: RVO:61389021 Keywords : Plasma potential * electron temperature * bi-Maxwellian EEDF * Divertor Langmuir probes * three- and four-parameter probe techniques * first-derivative probe technique Subject RIV: BL - Plasma and Gas Discharge Physics OBOR OECD: 2.11 Other engineering and technologies Impact factor: 1.220, year: 2016 http://iopscience.iop.org/article/10.1088/1748-0221/13/04/P04005/meta

  11. Contamination-free sounding rocket Langmuir probe

    Science.gov (United States)

    Amatucci, W. E.; Schuck, P. W.; Walker, D. N.; Kintner, P. M.; Powell, S.; Holback, B.; Leonhardt, D.

    2001-04-01

    A technique for removing surface contaminants from a sounding rocket spherical Langmuir probe is presented. Contamination layers present on probe surfaces can skew the collected data, resulting in the incorrect determination of plasma parameters. Despite following the usual probe cleaning techniques that are used prior to a launch, the probe surface can become coated with layers of adsorbed neutral gas in less than a second when exposed to atmosphere. The laboratory tests reported here show that by heating the probe from the interior using a small halogen lamp, adsorbed neutral particles can be removed from the probe surface, allowing accurate plasma parameter measurements to be made.

  12. Uropathogenic Escherichia coli virulence genes: invaluable approaches for designing DNA microarray probes.

    Science.gov (United States)

    Jahandeh, Nadia; Ranjbar, Reza; Behzadi, Payam; Behzadi, Elham

    2015-01-01

    The pathotypes of uropathogenic Escherichia coli (UPEC) cause different types of urinary tract infections (UTIs). The presence of a wide range of virulence genes in UPEC enables us to design appropriate DNA microarray probes. These probes, which are used in DNA microarray technology, provide us with an accurate and rapid diagnosis and definitive treatment in association with UTIs caused by UPEC pathotypes. The main goal of this article is to introduce the UPEC virulence genes as invaluable approaches for designing DNA microarray probes. Main search engines such as Google Scholar and databases like NCBI were searched to find and study several original pieces of literature, review articles, and DNA gene sequences. In parallel with in silico studies, the experiences of the authors were helpful for selecting appropriate sources and writing this review article. There is a significant variety of virulence genes among UPEC strains. The DNA sequences of virulence genes are fabulous patterns for designing microarray probes. The location of virulence genes and their sequence lengths influence the quality of probes. The use of selected virulence genes for designing microarray probes gives us a wide range of choices from which the best probe candidates can be chosen. DNA microarray technology provides us with an accurate, rapid, cost-effective, sensitive, and specific molecular diagnostic method which is facilitated by designing microarray probes. Via these tools, we are able to have an accurate diagnosis and a definitive treatment regarding UTIs caused by UPEC pathotypes.

  13. Fiber-based hybrid probe for non-invasive cerebral monitoring in neonatology

    Science.gov (United States)

    Rehberger, Matthias; Giovannella, Martina; Pagliazzi, Marco; Weigel, Udo; Durduran, Turgut; Contini, Davide; Spinelli, Lorenzo; Pifferi, Antonio; Torricelli, Alessandro; Schmitt, Robert

    2015-07-01

    Improved cerebral monitoring systems are needed to prevent preterm infants from long-term cognitive and motor restrictions. Combining advanced near-infrared diffuse spectroscopy measurement technologies, time-resolved spectroscopy (TRS) and diffuse correlation spectroscopy (DCS) will introduce novel indicators of cerebral oxygen metabolism and blood flow for neonatology. For non-invasive sensing a fiber-optical probe is used to send and receive light from the infant head. In this study we introduce a new fiber-based hybrid probe that is designed for volume production. The probe supports TRS and DCS measurements in a cross geometry, thus both technologies gain information on the same region inside the tissue. The probe is highly miniaturized to perform cerebral measurements on heads of extreme preterm infants down to head diameters of 6cm. Considerations concerning probe production focus on a reproducible accuracy in shape and precise optical alignment. In this way deviations in measurement data within a series of probes should be minimized. In addition to that, requirements for clinical use like robustness and hygiene are considered. An additional soft-touching sleeve made of FDA compatible silicone allows for a flexible attachment with respect to the individual anatomy of each patient. We present the technical concept of the hybrid probe and corresponding manufacturing methods. A prototype of the probe is shown and tested on tissue phantoms as well as in vivo to verify its operational reliability.

  14. Probe code: a set of programs for processing and analysis of the left ventricular function - User's manual

    International Nuclear Information System (INIS)

    Piva, R.M.V.

    1987-01-01

    The User's Manual of the Probe Code is an addendum to the M.Sc. thesis entitled A Microcomputer System of Nuclear Probe to Check the Left Ventricular Function. The Probe Code is a software which was developed for processing and off-line analysis curves from the Left Ventricular Function, that were obtained in vivo. These curves are produced by means of an external scintigraph probe, which was collimated and put on the left ventricule, after a venous inoculation of Tc-99 m. (author)

  15. Flexible probe for measuring local conductivity variations in Li-ion electrode films

    Science.gov (United States)

    Hardy, Emilee; Clement, Derek; Vogel, John; Wheeler, Dean; Mazzeo, Brian

    2018-04-01

    Li-ion battery performance is governed by electronic and ionic properties of the battery. A key metric that characterizes Li-ion battery cell performance is the electronic conductivity of the electrodes, which are metal foils with thin coatings of electrochemically active materials. To accurately measure the spatial variation of electronic conductivity of these electrodes, a micro-four-line probe (μ4LP) was designed and used to non-destructively measure the properties of commercial-quality Li-ion battery films. This previous research established that the electronic conductivity of film electrodes is not homogeneous throughout the entirety of the deposited film area. In this work, a micro-N-line probe (μNLP) and a flexible micro-flex-line probe (μFLP) were developed to improve the non-destructive micro-scale conductivity measurements that we can take. These devices were validated by comparing test results to that of the predecessor, the micro-four-line probe (μ4LP), on various commercial-quality Li-ion battery electrodes. Results show that there is significant variation in conductivity on a millimeter and even micrometer length scale through the electrode film. Compared to the μ4LP, the μNLP and μFLP also introduce additional measurement configuration possibilities, while providing a more robust design. Researchers and manufacturers can use these probes to identify heterogeneity in their electrodes during the fabrication process, which will lead to the development of better batteries.

  16. Technology Transfer

    Science.gov (United States)

    Smith, Nanette R.

    1995-01-01

    The objective of this summer's work was to attempt to enhance Technology Application Group (TAG) ability to measure the outcomes of its efforts to transfer NASA technology. By reviewing existing literature, by explaining the economic principles involved in evaluating the economic impact of technology transfer, and by investigating the LaRC processes our William & Mary team has been able to lead this important discussion. In reviewing the existing literature, we identified many of the metrics that are currently being used in the area of technology transfer. Learning about the LaRC technology transfer processes and the metrics currently used to track the transfer process enabled us to compare other R&D facilities to LaRC. We discuss and diagram impacts of technology transfer in the short run and the long run. Significantly, it serves as the basis for analysis and provides guidance in thinking about what the measurement objectives ought to be. By focusing on the SBIR Program, valuable information regarding the strengths and weaknesses of this LaRC program are to be gained. A survey was developed to ask probing questions regarding SBIR contractors' experience with the program. Specifically we are interested in finding out whether the SBIR Program is accomplishing its mission, if the SBIR companies are providing the needed innovations specified by NASA and to what extent those innovations have led to commercial success. We also developed a survey to ask COTR's, who are NASA employees acting as technical advisors to the SBIR contractors, the same type of questions, evaluating the successes and problems with the SBIR Program as they see it. This survey was developed to be implemented interactively on computer. It is our hope that the statistical and econometric studies that can be done on the data collected from all of these sources will provide insight regarding the direction to take in developing systematic evaluations of programs like the SBIR Program so that they can

  17. Oxidation of hydrogen-passivated silicon surfaces by scanning near-field optical lithography using uncoated and aluminum-coated fiber probes

    DEFF Research Database (Denmark)

    Madsen, Steen; Bozhevolnyi, Sergey I.; Birkelund, Karen

    1997-01-01

    Optically induced oxidation of hydrogen-passivated silicon surfaces using a scanning near-field optical microscope was achieved with both uncoated and aluminum-coated fiber probes. Line scans on amorphous silicon using uncoated fiber probes display a three-peak profile after etching in potassium...... hydroxide. Numerical simulations of the electromagnetic field around the probe-sample interaction region are used to explain the experimental observations. With an aluminum-coated fiber probe, lines of 35 nm in width were transferred into the amorphous silicon layer. (C) 1997 American Institute of Physics....

  18. Eddy current probe and method for flaw detection in metals

    Science.gov (United States)

    Watjen, John P.

    1987-06-23

    A flaw detecting system is shown which includes a probe having a pair of ferrite cores with in-line gaps in close proximity to each other. An insulating, non-magnetic, non-conducting holder fills the gaps and supports the ferrite cores in a manner such that the cores form a generally V-shape. Each core is provided with an excitation winding and a detection winding. The excitation windings are connected in series or parallel with an rf port for connection thereof to a radio frequency source. The detection windings, which are differentially wound, are connected in series circuit to a detector port for connection to a voltage measuring instrument. The ferrite cores at the in-line gaps directly engage the metal surface of a test piece, and the probe is scanned along the test piece. In the presence of a flaw in the metal surface the detection winding voltages are unbalanced, and the unbalance is detected by the voltage measuring instrument. The insulating holder is provided with a profile which conforms to that of a prominent feature of the test piece to facilitate movement of the probe along the feature, typically an edge or a corner.

  19. A fast scanning probe for DIII--D

    International Nuclear Information System (INIS)

    Watkins, J.G.; Salmonson, J.; Moyer, R.; Doerner, R.; Lehmer, R.; Schmitz, L.; Hill, D.N.

    1992-01-01

    A fast reciprocating probe has been developed for DIII--D which can penetrate the separatrix during H mode with up to 5 MW of NBI heating. The probe has been designed to carry various sensor tips into the scrape-off layer at a velocity of 3 m/s and dwell motionless for a programmed period of time. The driving force is provided by a pneumatic cylinder charged with helium to facilitate greater mass flow. The first series of experiments have been done using a Langmuir probe head with five graphite tips to measure radial profiles of n e , T e , φ f , n e , and φ f . The amplitude and phase of the fluctuating quantities are measured by using specially constructed vacuum compatible 5-kV coaxial transmission lines which allow us to extend the measurements into the MHz range. TTZ ceramic bearings and fast stroke bellows were also specially designed for the DIII--D probe. Initial measurements will be presented

  20. MIB Probes for measurements of particle and energy fluxes in plasma of Wendelstein 7-X

    Science.gov (United States)

    Demidov, V. I.; Koepke, M. E.; Kurlyandskaya, I. P.; Raitses, Y.

    2014-10-01

    Magnetically insulated baffled (MIB) probes and probe arrays that share the simplicity of simple Langmuir probes but supersede them in their ability to make real-time measurements of plasma potential, temperature and energy/particle fluxes in W7-X stellarator plasma are being developed. The probes offer the advantages of direct measurements of the plasma fluid observables, while being non-emitting and electrically floating. The principle of operation of the probe is based on the dependence of the voltage drop in the plasma-probe sheath on the direction of the local magnetic field. The core technology for these probes rests with the use of a special baffling configuration such that electron current to the probe is fully controllable in the closed, open or partially open orientation, by a simple rotation of the baffle with respect to the magnetic field alignment in the plasma. The baffled-probe designs proposed for edge diagnostics will increase the capability to characterize separately plasma properties in real-time for understanding of underlying physics in the edge plasma.

  1. Graphical representation of ribosomal RNA probe accessibility data using ARB software package

    Directory of Open Access Journals (Sweden)

    Amann Rudolf

    2005-03-01

    Full Text Available Abstract Background Taxon specific hybridization probes in combination with a variety of commonly used hybridization formats nowadays are standard tools in microbial identification. A frequently applied technology, fluorescence in situ hybridization (FISH, besides single cell identification, allows the localization and functional studies of the microbial community composition. Careful in silico design and evaluation of potential oligonucleotide probe targets is therefore crucial for performing successful hybridization experiments. Results The PROBE Design tools of the ARB software package take into consideration several criteria such as number, position and quality of diagnostic sequence differences while designing oligonucleotide probes. Additionally, new visualization tools were developed to enable the user to easily examine further sequence associated criteria such as higher order structure, conservation, G+C content, transition-transversion profiles and in situ target accessibility patterns. The different types of sequence associated information (SAI can be visualized by user defined background colors within the ARB primary and secondary structure editors as well as in the PROBE Match tool. Conclusion Using this tool, in silico probe design and evaluation can be performed with respect to in situ probe accessibility data. The evaluation of proposed probe targets with respect to higher-order rRNA structure is of importance for successful design and performance of in situ hybridization experiments. The entire ARB software package along with the probe accessibility data is available from the ARB home page http://www.arb-home.de.

  2. Application of DNA fingerprints for cell-line individualization.

    OpenAIRE

    Gilbert, D A; Reid, Y A; Gail, M H; Pee, D; White, C; Hay, R J; O'Brien, S J

    1990-01-01

    DNA fingerprints of 46 human cell lines were derived using minisatellite probes for hypervariable genetic loci. The incidence of 121 HaeIII DNA fragments among 33 cell lines derived from unrelated individuals was used to estimate allelic and genotypic frequencies for each fragment and for composite individual DNA fingerprints. We present a quantitative estimate of the extent of genetic difference between individuals, an estimate based on the percentage of restriction fragments at which they d...

  3. Measurement of Safety Factor Using Hall Probes on CASTOR Tokamak

    Czech Academy of Sciences Publication Activity Database

    Kovařík, Karel; Ďuran, Ivan; Bolshakova, I.; Holyaka, R.; Erashok, V.

    2006-01-01

    Roč. 56, suppl.B (2006), s. 104-110 ISSN 0011-4626. [Symposium on Plasma Physics and Technology/22nd./. Praha, 26.6.2006-29.6.2006] R&D Projects: GA AV ČR(CZ) KJB100430504 Institutional research plan: CEZ:AV0Z20430508 Keywords : Plasma * tokamak * safety factor * hall probe Subject RIV: BL - Plasma and Gas Discharge Physics Impact factor: 0.568, year: 2006

  4. Label-free biosensing with functionalized nanopipette probes

    OpenAIRE

    Umehara, Senkei; Karhanek, Miloslav; Davis, Ronald W.; Pourmand, Nader

    2009-01-01

    Nanopipette technology can uniquely identify biomolecules such as proteins based on differences in size, shape, and electrical charge. These differences are determined by the detection of changes in ionic current as the proteins interact with the nanopipette tip coated with probe molecules. Here we show that electrostatic, biotin-streptavidin, and antibody-antigen interactions on the nanopipette tip surface affect ionic current flowing through a 50-nm pore. Highly charged polymers interacting...

  5. ELECTRIC PROBE INVESTIGATION OF ARC ANODE REGION IN PLASMA TORCH

    Czech Academy of Sciences Publication Activity Database

    Chumak, Oleksiy; Hrabovský, Milan; Kavka, Tetyana

    2006-01-01

    Roč. 10, č. 4 (2006), s. 515-524 ISSN 1093-3611. [High technology plasma processes. Saint-Petersburg, 27.5.2006-4.6.2006] R&D Projects: GA ČR GA202/05/0669 Institutional research plan: CEZ:AV0Z20430508 Keywords : plasma torch * arc * anode attachment * restrike * electric probes Subject RIV: BL - Plasma and Gas Discharge Physics Impact factor: 0.343, year: 2006

  6. Listener: a probe into information based material specification

    DEFF Research Database (Denmark)

    Ramsgaard Thomsen, Mette; Karmon, Ayelet

    2011-01-01

    This paper presents the thinking and making of the architectural research probe Listener. Developed as an interdisciplinary collaboration between textile design and architecture, Listener explores how information based fabrication technologies are challenging the material practices of architecture....... The paper investigates how textile design can be understood as a model for architectural production providing new strategies for material specification and allowing the thinking of material as inherently variegated and performative. The paper traces the two fold information based strategies present...

  7. Cantilevered probe detector with piezoelectric element

    Science.gov (United States)

    Adams, Jesse D; Sulchek, Todd A; Feigin, Stuart C

    2013-04-30

    A disclosed chemical detection system for detecting a target material, such as an explosive material, can include a cantilevered probe, a probe heater coupled to the cantilevered probe, and a piezoelectric element disposed on the cantilevered probe. The piezoelectric element can be configured as a detector and/or an actuator. Detection can include, for example, detecting a movement of the cantilevered probe or a property of the cantilevered probe. The movement or a change in the property of the cantilevered probe can occur, for example, by adsorption of the target material, desorption of the target material, reaction of the target material and/or phase change of the target material. Examples of detectable movements and properties include temperature shifts, impedance shifts, and resonant frequency shifts of the cantilevered probe. The overall chemical detection system can be incorporated, for example, into a handheld explosive material detection system.

  8. Computer modelling of eddy current probes

    International Nuclear Information System (INIS)

    Sullivan, S.P.

    1992-01-01

    Computer programs have been developed for modelling impedance and transmit-receive eddy current probes in two-dimensional axis-symmetric configurations. These programs, which are based on analytic equations, simulate bobbin probes in infinitely long tubes and surface probes on plates. They calculate probe signal due to uniform variations in conductor thickness, resistivity and permeability. These signals depend on probe design and frequency. A finite element numerical program has been procured to calculate magnetic permeability in non-linear ferromagnetic materials. Permeability values from these calculations can be incorporated into the above analytic programs to predict signals from eddy current probes with permanent magnets in ferromagnetic tubes. These programs were used to test various probe designs for new testing applications. Measurements of magnetic permeability in magnetically biased ferromagnetic materials have been performed by superimposing experimental signals, from special laboratory ET probes, on impedance plane diagrams calculated using these programs. (author). 3 refs., 2 figs

  9. Where do pulse oximeter probes break?

    Science.gov (United States)

    Crede, S; Van der Merwe, G; Hutchinson, J; Woods, D; Karlen, W; Lawn, J

    2014-06-01

    Pulse oximetry, a non-invasive method for accurate assessment of blood oxygen saturation (SPO2), is an important monitoring tool in health care facilities. However, it is often not available in many low-resource settings, due to expense, overly sophisticated design, a lack of organised procurement systems and inadequate medical device management and maintenance structures. Furthermore medical devices are often fragile and not designed to withstand the conditions of low-resource settings. In order to design a probe, better suited to the needs of health care facilities in low-resource settings this study aimed to document the site and nature of pulse oximeter probe breakages in a range of different probe designs in a low to middle income country. A retrospective review of job cards relating to the assessment and repair of damaged or faulty pulse oximeter probes was conducted at a medical device repair company based in Cape Town, South Africa, specializing in pulse oximeter probe repairs. 1,840 job cards relating to the assessment and repair of pulse oximeter probes were reviewed. 60.2 % of probes sent for assessment were finger-clip probes. For all probes, excluding the neonatal wrap probes, the most common point of failure was the probe wiring (>50 %). The neonatal wrap most commonly failed at the strap (51.5 %). The total cost for quoting on the broken pulse oximeter probes and for the subsequent repair of devices, excluding replacement components, amounted to an estimated ZAR 738,810 (USD $98,508). Improving the probe wiring would increase the life span of pulse oximeter probes. Increasing the life span of probes will make pulse oximetry more affordable and accessible. This is of high priority in low-resource settings where frequent repair or replacement of probes is unaffordable or impossible.

  10. Solar Probe Cup: Laboratory Performance

    Science.gov (United States)

    Case, A. W.; Kasper, J. C.; Korreck, K. E.; Stevens, M. L.; Larson, D. E.; Wright, K. H., Jr.; Gallagher, D. L.; Whittlesey, P. L.

    2017-12-01

    The Solar Probe Cup (SPC) is a Faraday Cup instrument that will fly on the Paker Solar Probe (PSP) spacecraft, orbiting the Sun at as close as 9.86 solar radii. The SPC instrument is designed to measure the thermal solar wind plasma (protons, alphas, and electrons) that will be encountered throughout its close encounter with the Sun. Due to the solar wind flow being primarily radial, the SPC instrument is pointed directly at the Sun, resulting in an extreme thermal environment that must be tolerated throughout the primary data collection phase. Laboratory testing has been performed over the past 6 months to demonstrate the instrument's performance relative to its requirements, and to characterize the measurements over the expected thermal range. This presentation will demonstrate the performance of the instrument as measured in the lab, describe the operational configurations planned for flight, and discuss the data products that will be created.

  11. Electrostatic probes in luminescent discharges

    International Nuclear Information System (INIS)

    Cunha Raposo, C. da.

    1980-01-01

    A system to produce luminescent type plasma by continuos discharge and ionization by high frequency was constructed. The ionization was done in the air and in the argon under pressures from 3 to 10 mmHg. The parameters of a non magnetized collisional plasma and the parameters of a magnetized plasma such as, density, eletron temperature and potential, using a Langmuir probe with plane geometry, were determined. (M.C.K.) [pt

  12. DNA Probe for Lactobacillus delbrueckii

    OpenAIRE

    Delley, Michèle; Mollet, Beat; Hottinger, Herbert

    1990-01-01

    From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific DNA probe. Dot-blot hybridizations with over 40 different Lactobacillus strains showed that this clone specifically recognizes L. delbrueckii subsp. delbrueckii, bulgaricus, and lactis. The sensitivity of the method was tested by using an α-32P-l...

  13. Atomic beams probe surface vibrations

    International Nuclear Information System (INIS)

    Robinson, A.L.

    1982-01-01

    In the last two years, surface scientist have begun trying to obtain the vibrational frequencies of surface atoms in both insulating and metallic crystals from beams of helium atoms. It is the inelastic scattering that researchers use to probe surface vibrations. Inelastic atomic beam scattering has only been used to obtain vibrational frequency spectra from clean surfaces. Several experiments using helium beams are cited. (SC)

  14. Distance probes of dark energy

    Energy Technology Data Exchange (ETDEWEB)

    Kim, A. G.; Padmanabhan, N.; Aldering, G.; Allen, S. W.; Baltay, C.; Cahn, R. N.; D’Andrea, C. B.; Dalal, N.; Dawson, K. S.; Denney, K. D.; Eisenstein, D. J.; Finley, D. A.; Freedman, W. L.; Ho, S.; Holz, D. E.; Kasen, D.; Kent, S. M.; Kessler, R.; Kuhlmann, S.; Linder, E. V.; Martini, P.; Nugent, P. E.; Perlmutter, S.; Peterson, B. M.; Riess, A. G.; Rubin, D.; Sako, M.; Suntzeff, N. V.; Suzuki, N.; Thomas, R. C.; Wood-Vasey, W. M.; Woosley, S. E.

    2015-03-01

    This document presents the results from the Distances subgroup of the Cosmic Frontier Community Planning Study (Snowmass 2013). We summarize the current state of the field as well as future prospects and challenges. In addition to the established probes using Type Ia supernovae and baryon acoustic oscillations, we also consider prospective methods based on clusters, active galactic nuclei, gravitational wave sirens and strong lensing time delays.

  15. Lasers probe the atomic nucleus

    International Nuclear Information System (INIS)

    Eastham, D.

    1986-01-01

    The article is contained in a booklet on the Revised Nuffield Advanced Physics Course, and concentrates on two techniques to illustrate how lasers probe the atomic nucleus. Both techniques employ resonance fluorescence spectroscopy for obtaining atomic transition energies. The first uses lasers to determine the change in the nuclear charge radius with isotope, the second concerns the use of lasers for ultrasensitive detection of isotopes and elements. The application of lasers in resonance ionization spectroscopy and proton decay is also described. (UK)

  16. Development of Korea telecommunication technology

    International Nuclear Information System (INIS)

    1992-06-01

    It concentrates on development of Korea telecommunication technology, which is made up seven chapters. It gives description of manual central telephone exchange or private automatic telephone exchange, transmission technology on wire line and cable line technology and optical transmission, radio communication technology on mobile and natural satellite communication, network technology with intelligent network, broadband ISDN and packet switched Data Network, terminal technology with telephone and data communication terminal and development of Information Technology in Korea. It has an appendix about development of military communication system.

  17. VT Electric Transmission Line Corridors - corridor lines

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) The ELTRN layer depicts electric transmission line corridors in Vermont. Various methods have been used to digitize features. The data layer...

  18. Modeling and interpretation of line observations*

    Directory of Open Access Journals (Sweden)

    Kamp Inga

    2015-01-01

    Full Text Available Models for the interpretation of line observations from protoplanetary disks are summarized. The spectrum ranges from 1D LTE slab models to 2D thermo-chemical radiative transfer models and their use depends largely on the type/nature of observational data that is analyzed. I discuss the various types of observational data and their interpretation in the context of disk physical and chemical properties. The most simple spatially and spectral unresolved data are line fluxes, which can be interpreted using so-called Boltzmann diagrams. The interpretation is often tricky due to optical depth and non-LTE effects and requires care. Line profiles contain kinematic information and thus indirectly the spatial origin of the emission. Using series of line profiles, we can for example deduce radial temperature gradients in disks (CO pure rotational ladder. Spectro-astrometry of e.g. CO ro-vibrational line profiles probes the disk structure in the 1–30 AU region, where planet formation through core accretion should be most efficient. Spatially and spectrally resolved line images from (submm interferometers are the richest datasets we have to date and they enable us to unravel exciting details of the radial and vertical disk structure such as winds and asymmetries.

  19. Probing a gravitational cat state

    International Nuclear Information System (INIS)

    Anastopoulos, C; Hu, B L

    2015-01-01

    We investigate the nature of a gravitational two-state system (G2S) in the simplest setup in Newtonian gravity. In a quantum description of matter a single motionless massive particle can in principle be in a superposition state of two spatially separated locations. This superposition state in gravity, or gravitational cat state, would lead to fluctuations in the Newtonian force exerted on a nearby test particle. The central quantity of importance for this inquiry is the energy density correlation. This corresponds to the noise kernel in stochastic gravity theory, evaluated in the weak field nonrelativistic limit. In this limit quantum fluctuations of the stress–energy tensor manifest as the fluctuations of the Newtonian force. We describe the properties of such a G2S system and present two ways of measuring the cat state for the Newtonian force, one by way of a classical probe, the other a quantum harmonic oscillator. Our findings include: (i) mass density fluctuations persist even in single particle systems, and they are of the same order of magnitude as the mean; (ii) a classical probe generically records a non-Markovian fluctuating force; (iii) a quantum probe interacting with the G2S system may undergo Rabi oscillations in a strong coupling regime. This simple prototypical gravitational quantum system could provide a robust testing ground to compare predictions from alternative quantum theories, since the results reported here are based on standard quantum mechanics and classical gravity. (paper)

  20. The Van Allen Probes mission

    CERN Document Server

    Burch, James

    2014-01-01

    This collection of articles provides broad and detailed information about NASA’s Van Allen Probes (formerly known as the Radiation Belt Storm Probes) twin-spacecraft Earth-orbiting mission. The mission has the objective of achieving predictive understanding of the dynamic, intense, energetic, dangerous, and presently unpredictable belts of energetic particles that are magnetically trapped in Earth’s space environment above the atmosphere. It documents the science of the radiation belts and the societal benefits of achieving predictive understanding. Detailed information is provided about the Van Allen Probes mission design, the spacecraft, the science investigations, and the onboard instrumentation that must all work together to make unprecedented measurements within a most unforgiving environment, the core of Earth’s most intense radiation regions.
 This volume is aimed at graduate students and researchers active in space science, solar-terrestrial interactions and studies of the up...