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Sample records for line characterization project

  1. K Basin sandfilter backwash line characterization project, analytical results for Campaign 20

    STEEN, F.H.

    1999-01-01

    Sample 112KWBMF was taken from the K West Sandfilter Backwash Pit on June 1 , 1999, and received by 222-S Laboratory on June 2,1999. Analyses were performed on sample 112KWBMF in accordance with Letter of Instruction for K Basins Sandfilter Backwash Line Samples (LOI) in support of the K Basin Sandfilter Backwash Line Characterization Project

  2. K basin sandfilter backwash line characterization project, analytical results for campaign 15

    Steen, F.H.

    1999-01-01

    Sample 183KWBMF was taken from the K West Sandfilter Backwash Pit on August 28, 1998 and received by 222-S Laboratory on August 28, 1998. Analyses were performed in accordance with ''Letter of Instruction for K Basins Sandfilter Backwash Line Samples'' (LOI) in support of the K Basin Sandfilter Backwash Line Characterization Project

  3. K Basin Sandfilter Backwash Line Characterization Project and Analytical Results for Campaign 24

    STEEN, F.H.

    1999-01-01

    Sample 203KWBMF was taken from the K West Sandfilter Backwash Pit on November 4, 1999 and received by 2224 Laboratory on November 4, 1999. Analyses were performed on sample 203KWBMF in accordance with ''Letter of Instruction for K Basins sandfilter Backwash Line Samples'' (LOI) in support of the K Basin Sandfilter Backwash Line Characterization Project

  4. Spent Pot Lining Characterization Framework

    Ospina, Gustavo; Hassan, Mohamed I.

    2017-09-01

    Spent pot lining (SPL) management represents a major concern for aluminum smelters. There are two key elements for spent pot lining management: recycling and safe storage. Spent pot lining waste can potentially have beneficial uses in co-firing in cement plants. Also, safe storage of SPL is of utmost importance. Gas generation of SPL reaction with water and ignition sensitivity must be studied. However, determining the feasibility of SPL co-firing and developing the required procedures for safe storage rely on determining experimentally all the necessary SPL properties along with the appropriate test methods, recognized by emissions standards and fire safety design codes. The applicable regulations and relevant SPL properties for this purpose are presented along with the corresponding test methods.

  5. Coaxial tube array space transmission line characterization

    Switzer, C.A.; Bents, D.J.

    1987-01-01

    The coaxial tube array tether/transmission line used to connect an SP-100 nuclear power system to the space station was characterized over the range of reactor-to-platform separation distances of 1 to 10 km. Characterization was done with respect to array performance, physical dimensions and masses. Using a fixed design procedure, a family of designs was generated for the same power level (300 kWe), power loss (1.5 percent), and meteoroid survival probability (99.5 percent over 10 yr). To differentiate between vacuum insulated and gas insulated lines, two different maximum values of the E field were considered: 20 kV/cm (appropriate to vacuum insulation) and 50 kV/cm (compressed SF6). Core conductor, tube, bumper, standoff, spacer and bumper support dimensions, and masses were also calculated. The results of the characterization show mainly how transmission line size and mass scale with reactor-to-platform separation distance

  6. Aerosol characterization during project POLINAT

    Hagen, D.E.; Hopkins, A.R.; Paladino, J.D.; Whitefield, P.D. [Missouri Univ., Rolla, MO (United States). Cloud and Aerosol Sciences Lab.; Lilenfeld, H.V. [McDonnell Douglas Aerospace-East, St. Louis, MO (United States)

    1997-12-31

    The objectives of the aerosol/particulate characterization measurements of project POLINAT (POLlution from aircraft emissions In the North ATlantic flight corridor) are: to search for aerosol/particulate signatures of air traffic emissions in the region of the North Atlantic Flight Corridor; to search for the aerosol/particulate component of large scale enhancement (`corridor effects`) of air traffic related species in the North Atlantic region; to determine the effective emission indices for the aerosol/particulate component of engine exhaust in both the near and far field of aircraft exhaust plumes; to measure the dispersion and transformation of the aerosol/particulate component of aircraft emissions as a function of ambient condition; to characterize background levels of aerosol/particulate concentrations in the North Atlantic Region; and to determine effective emission indices for engine exhaust particulates for regimes beyond the jet phase of plume expansion. (author) 10 refs.

  7. Aerosol characterization during project POLINAT

    Hagen, D E; Hopkins, A R; Paladino, J D; Whitefield, P D [Missouri Univ., Rolla, MO (United States). Cloud and Aerosol Sciences Lab.; Lilenfeld, H V [McDonnell Douglas Aerospace-East, St. Louis, MO (United States)

    1998-12-31

    The objectives of the aerosol/particulate characterization measurements of project POLINAT (POLlution from aircraft emissions In the North ATlantic flight corridor) are: to search for aerosol/particulate signatures of air traffic emissions in the region of the North Atlantic Flight Corridor; to search for the aerosol/particulate component of large scale enhancement (`corridor effects`) of air traffic related species in the North Atlantic region; to determine the effective emission indices for the aerosol/particulate component of engine exhaust in both the near and far field of aircraft exhaust plumes; to measure the dispersion and transformation of the aerosol/particulate component of aircraft emissions as a function of ambient condition; to characterize background levels of aerosol/particulate concentrations in the North Atlantic Region; and to determine effective emission indices for engine exhaust particulates for regimes beyond the jet phase of plume expansion. (author) 10 refs.

  8. 77 FR 35366 - Albany-Eugene Transmission Line Rebuild Project

    2012-06-13

    ... materials, removing vegetation including danger trees, and revegetating areas disturbed by construction... implement the Proposed Action Alternative, based on the Albany-Eugene Transmission Line Rebuild Project (DOE...

  9. Metro orange line BRT project evaluation.

    2011-10-01

    In partnership with the Los Angeles County Metropolitan Transportation Authority (Metro) and the Federal Transit Administration (FTA), the National Bus Rapid Transit Institute (NBRTI) conducted an evaluation of the Metro Orange Line BRT service, whic...

  10. Characterize Human Forward Contamination Project

    Rucker, Michelle

    2015-01-01

    Let's face it: wherever we go, we will inevitably carry along the little critters that live in and on us. Conventional wisdom has long held that it's unlikely those critters could survive the space environment, but in 2007 microscopic animals called Tardigrades survived exposure to space and in 2008 Cyanobacteria lived for 548 days outside the International Space Station (ISS). But what about the organisms we might reasonably expect a crewed spacecraft to leak or vent? Do we even know what they are? How long might our tiny hitch-hikers survive in close proximity to a warm spacecraft that periodically leaks/vents water or oxygen-and how might they mutate with long-duration exposure? Unlike the Mars rovers that we cleaned once and sent on their way, crew members will provide a constantly regenerating contaminant source. Are we prepared to certify that we can meet forward contamination protocols as we search for life at new destinations? This project has four technical objectives: 1. TEST: Develop a test plan to leverage existing equipment (i.e. ISS) to characterize the kinds of organisms we can reasonably expect pressurized, crewed volumes to vent or leak overboard; as part of testing, we'll need to develop an Extravehicular Activity (EVA)-compatible tool that can withstand the pressure and temperature extremes of space, as well as collect, separate, and store multiple samples; 2. ANALYSIS: Develop an analysis plan to study those organisms in relevant destination environments, including spacecraft-induced conditions; 3. MODEL: Develop a modeling plan to model organism transport mechanisms in relevant destination environments; 4. SHARE: Develop a plan to disseminate findings and integrate recommendations into exploration requirements & ops. In short, we propose a system engineering approach to roadmap the necessary experiments, analysis, and modeling up front--rather than try to knit together disparate chunks of data into a sensible conclusion after the fact.

  11. Tank waste remediation system characterization project quality policies. Revision 1

    Trimble, D.J.

    1995-01-01

    These Quality Policies (QPs) describe the Quality Management System of the Tank Waste Characterization Project (hereafter referred to as the Characterization Project), Tank Waste Remediation System (TWRS), Westinghouse Hanford Company (WHC). The Quality Policies and quality requirements described herein are binding on all Characterization Project organizations. To achieve quality, the Characterization Project management team shall implement this Characterization Project Quality Management System

  12. Radioisotope Characterization of HB Line Low Activity Waste

    Snyder, S.J.

    1999-01-01

    The purpose of this document is to provide a physical, chemical, hazardous and radiological characterization of Low-Level Waste (LLW) generated in HB-Line as required by the 1S Manual, Savannah River Site Waste Acceptance Criteria Manual

  13. Characterization of superconducting transmission line resonators

    Goetz, Jan; Summer, Philipp; Meier, Sebastian; Haeberlein, Max; Wulschner, Karl Friedrich; Eder, Peter; Fischer, Michael; Schwarz, Manuel; Deppe, Frank; Fedorov, Kirill; Huebl, Hans; Menzel, Edwin [Walther-Meissner-Institut, Bayerische Akademie der Wissenschaften, Garching (Germany); Physik-Department, TU Muenchen, Garching (Germany); Krawczyk, Marta; Marx, Achim [Walther-Meissner-Institut, Bayerische Akademie der Wissenschaften, Garching (Germany); Baust, Alexander; Xie, Edwar; Zhong, Ling; Gross, Rudolf [Walther-Meissner-Institut, Bayerische Akademie der Wissenschaften, Garching (Germany); Physik-Department, TU Muenchen, Garching (Germany); Nanosystems Initiative Munich (NIM), Muenchen (Germany)

    2015-07-01

    Superconducting transmission line resonators are widely used in circuit quantum electrodynamics experiments as quantum bus or storage devices. For these applications, long coherence times, which can be linked to the internal quality factor of the resonators, are crucial. Here, we show a systematic study of the internal quality factor of niobium thin film resonators. We analyze different cleaning methods and substrate parameters for coplanar waveguide as well as microstrip geometries. In addition, we investigate the impact of a niobium-aluminum interface which is necessary for galvanically coupled flux qubits made from aluminum. This interface can be avoided by fabricating the complete resonator-qubit structure using Al/AlO{sub x}/Al technology during fabrication.

  14. 77 FR 5489 - Identification of Human Cell Lines Project

    2012-02-03

    ... individual or species. With the advent of standardized, simple, and rapid methods for human cell line... project will undergo STR profiling, a DNA profiling method that examines/screens for STRs (DNA elements 2... distinct DNA profile and when the STR DNA fragment sizes are converted to numeric values, the DNA profiles...

  15. Safeguards and security benefits of project straight-line

    Jaeger, C.D.; Waddoups, I.G.

    1995-01-01

    As a result of a number of events the inventory of fissile materials no longer in nuclear weapons in the United States is increasing. This has led to a growing concern regarding the potential for theft and/or diversion and accountability of this material. Straight-Line is a project whose purpose is to demonstrate a site-independent system to monitor stored nuclear material (e.g. plutonium) and integrate the collection, processing and dissemination of information regarding this material. Safeguards and security (S ampersand S) goals of this project include data transfer of information on nuclear material to appropriate users to enhance S ampersand S, continuous on-line accountability, reduction of hands-on access to nuclear materials, incorporation of information security technologies, and early detection of tampering or unauthorized material movement. This paper addresses threat considerations, S ampersand S requirements, S ampersand S objectives, and issues for the Straight-Line project. S ampersand S features and benefits of this project are discussed with respect to existing item monitoring systems and/or other material tracking systems being developed

  16. Twisted sigma-model solitons on the quantum projective line

    Landi, Giovanni

    2018-04-01

    On the configuration space of projections in a noncommutative algebra, and for an automorphism of the algebra, we use a twisted Hochschild cocycle for an action functional and a twisted cyclic cocycle for a topological term. The latter is Hochschild-cohomologous to the former and positivity in twisted Hochschild cohomology results into a lower bound for the action functional. While the equations for the critical points are rather involved, the use of the positivity and the bound by the topological term lead to self-duality equations (thus yielding twisted noncommutative sigma-model solitons, or instantons). We present explicit nontrivial solutions on the quantum projective line.

  17. Project development and commercialization of on-line analysis systems

    Watt, J.S.

    1997-01-01

    A project team first in the Australian Atomic Energy Commission (AAEC) and since 1982 in CSIRO has developed many on-line analysis systems for the mineral and energy industries. The development of these projects has followed a common pattern of laboratory R and D, field trials, commercialisation and technology transfer. This successful pattern is illustrated using examples of the development of systems for the on-line analysis of mineral slurries, for determination of the ash content of coal on conveyors, and for determination of the flow rates of oil, water and gas in pipelines. The first two systems are licensed to Australian companies, Amdel Ltd and Mineral Control Instrumentation Ltd. Both systems are used by industry worldwide, and are the market leaders for radioisotope gauges in their application field. The third system, the multiphase flow meter, was licensed in 1997 to Kvaerner FSSL Ltd of Aberdeen. This meter has even greater potential than the other two systems for economic benefit from its used and for numbers of installations. The on-line analysis systems have been developed to increase the productivity of the Australian mineral and energy industries, and to provide economic benefit to Australia. The economic benefit sought is predominantly improved process control based on use of the instrument, rather than from its sale. Sales of instruments are significant, however, with about A$80 million from the analysis systems and their derivatives since the 1970s. Some of the issues associated with the development of the on-line analysis system are outlined

  18. Characterization Plan for Soils Around Drain Line PLA-100115

    D. Shanklin

    2006-05-24

    This Characterization Plan supports the Hazardous Waste Management Act/Resource Conservation and Recovery Act (HWMA/RCRA) closure of soils that may have been contaminated by releases from drain line PLA-100115, located within the Idaho Nuclear Technology and Engineering Center at the Idaho National Laboratory Site. The requirements to address the closure of soils contaminated by a potential release from this line in a characterization plan was identified in the "HWMA/RCRA Less Than 90-day Generator Closure Report for the VES-SFE-126."

  19. Gypsy Field project in reservoir characterization

    Castagna, John P.; Jr., O' Meara, Daniel J.

    2000-01-12

    The overall objective of this project was to use extensive Gypsy Field Laboratory and data as a focus for developing and testing reservoir characterization methods that are targeted at improved recovery of conventional oil. This report describes progress since project report DOE/BC/14970-7 and covers the period June 1997-September 1998 and represents one year of funding originally allocated for the year 1996. During the course of the work previously performed, high resolution geophysical and outcrop data revealed the importance of fractures at the Gypsy site. In addition, personnel changes and alternative funding (OCAST and oil company support of various kinds) allowed the authors to leverage DOE contributions and focus more on geophysical characterization.

  20. Fort Peck-Wolf Point transmission line project, Montana

    1992-01-01

    The primary objective of the project is to replace the existing 36-mile Fort Peck-Wolf Point transmission line which has reached the end of its useful service life. Presently, the overall condition of this existing section of the 47-year-old line is poor. Frequent repairs have been required because of the absence of overhead ground wires. The continued maintenance of the line will become more expensive and customer interruptions will persist because of the damage due to lightning. The expense of replacing shell rotted poles, and the concern for the safety of the maintenance personnel because of hazards caused by severe shell rot are also of primary importance. The operational and maintenance problems coupled with power system simulation studies, demonstrate the need for improvements to the Wolf Point area to serve area loads. Western's Wolf Point Substation is an important point of interconnection for the power output from the Fort Peck Dam to area loads as far away as Williston, North Dakota. The proposed transmission line replacement would assure that there will continue to be reliable transmission capacity available to serve area electrical loads, as well as provide a reliable second high-voltage transmission path from the Fort Peck generation to back-up a loss of the Fort Peck-Wolf Point 115-kV Line No. 1

  1. Establishment and characterization of rat portal myofibroblast cell lines.

    Michel Fausther

    Full Text Available The major sources of scar-forming myofibroblasts during liver fibrosis are activated hepatic stellate cells (HSC and portal fibroblasts (PF. In contrast to well-characterized HSC, PF remain understudied and poorly defined. This is largely due to the facts that isolation of rodent PF for functional studies is technically challenging and that PF cell lines had not been established. To address this, we have generated two polyclonal portal myofibroblast cell lines, RGF and RGF-N2. RGF and RGF-N2 were established from primary PF isolated from adult rat livers that underwent culture activation and subsequent SV40-mediated immortalization. Specifically, Ntpdase2/Cd39l1-sorted primary PF were used to generate the RGF-N2 cell line. Both cell lines were functionally characterized by RT-PCR, immunofluorescence, immunoblot and bromodeoxyuridine-based proliferation assay. First, immortalized RGF and RGF-N2 cells are positive for phenotypic myofibroblast markers alpha smooth muscle actin, type I collagen alpha-1, tissue inhibitor of metalloproteinases-1, PF-specific markers elastin, type XV collagen alpha-1 and Ntpdase2/Cd39l1, and mesenchymal cell marker ecto-5'-nucleotidase/Cd73, while negative for HSC-specific markers desmin and lecithin retinol acyltransferase. Second, both RGF and RGF-N2 cell lines are readily transfectable using standard methods. Finally, RGF and RGF-N2 cells attenuate the growth of Mz-ChA-1 cholangiocarcinoma cells in co-culture, as previously demonstrated for primary PF. Immortalized rat portal myofibroblast RGF and RGF-N2 cell lines express typical markers of activated PF-derived myofibroblasts, are suitable for DNA transfection, and can effectively inhibit cholangiocyte proliferation. Both RGF and RGF-N2 cell lines represent novel in vitro cellular models for the functional studies of portal (myofibroblasts and their contribution to the progression of liver fibrosis.

  2. Project development and commercialisation of on-line analysis systems

    Watt, J.S.

    2000-01-01

    A project team first in the Australian Atomic Energy Commission (AAEC) and since 1982 in CSIRO has developed many on-line analysis systems for the mineral and energy industries. The development of these projects, usually lasting 7-10 years, has followed a common pattern of laboratory R and D, field trials, commercialisation and technology transfer. This successful pattern is illustrated using examples of the development of systems for the on-line analysis of mineral slurries, for determination of the ash content of coal on conveyors, and for determination of the flow rates of oil, water and gas in pipelines. The first two systems, licensed to Australian companies, are used world-wide. They are now the market leaders for radioisotope gauges in their application field. The third, the multiphase flow meter, was licensed in 1997 to an international company. This meter has even greater potential than the other two systems for economic benefit from its use and for numbers of installations. (author)

  3. Canby Area Service Project substation and associated transmission line

    1992-02-01

    Bonneville Power Administration (BPA) provides power to Surprise Valley Electrification Corporation (SVEC) in Modoc County, California. BPA uses PacificCorp's substation and transmission facilities between Alturas and Canby, California to transfer power to SVEC's Canby Substation. In the next year, SVEC expects increased industrial, agricultural, and residential electric loads on their 69-kV transmission system south of Canby. SVEC's substation can accommodate only about 10 percent of the expected additional electric load. BPA's proposed action is intended to meet SVEC's increasing electric load. BPA proposes to meet SVEC's increasing energy load by tapping into BPA's existing BPA Malin-Warner 230-kV transmission line, and building an 7.9-mile transmission line to a new BPA substation. BPA proposes to build the new substation next to the west side of SVEC's Canby Substation (Figure 1). This new substation will allow SVEC to move the additional power over their existing transmission or distribution lines. This report is the environmental assessment of the potential impact of the proposed project. The assessment determined that no ''environmental impact statement'' is not required

  4. Gypsy Field Project in Reservoir Characterization

    John P. Castagna; William J. Lamb; Carlos Moreno; Roger Young; Lynn Soreghan

    2006-01-01

    The objective of the Gypsy Project was to properly calculate seismic attributes and integrate these into a reservoir characterization project. Significant progress was made on the project in four areas. (1) Attenuation: In order for seismic inversion for rock properties or calculation of seismic attributes used to estimate rock properties to be performed validly, it is necessary to deal with seismic data that has had true amplitude and frequency content restored to account for earth filtering effects that are generally not included in seismic reservoir characterization methodologies. This requires the accurate measurement of seismic attenuation, something that is rarely achieved in practice. It is hoped that such measurements may also provide additional independent seismic attributes for use in reservoir characterization studies. In 2000, we were concerned with the ground truthing of attenuation measurements in the vicinity of wells. Our approach to the problem is one of extracting as time varying wavelet and relating temporal variations in the wavelet to an attenuation model of the earth. This method has the advantage of correcting for temporal variations in the reflectivity spectrum of the earth which confound the spectral ratio methodology which is the most commonly applied means of measuring attenuation from surface seismic data. Part I of the report describes our efforts in seismic attenuation as applied to the Gypsy data. (2) Optimal Attributes: A bewildering array of seismic attributes is available to the reservoir geoscientist to try to establish correlations to rock properties. Ultimately, the use of such a large number of degrees of freedom in the search for correlations with limited well control leads to common misapplication of statistically insignificant results which yields invalid predictions. Cross-validation against unused wells can be used to recognize such problems, but does not offer a solution to the question of which attributes should be used

  5. Hydrologic site characterization - the UMTRA project approach

    Binkman, J.E.; Hoopes, J.R.

    1985-01-01

    The United States Environmental Protection Agency (EPA) Standards (40 CFR 192) require site characterization of the hydrogeologic regime at and around each Uranium Mill Tailings Remedial Action (UMTRA) Project site. Also, ''judgements on the possible need for remedial or protective actions for groundwater aquifers should be guided by relevant considerations described in EPA's hazardous waste management system (47 CFR 32274).'' To address those two sets of rules and regulations, a generic approach is being developed. Fourteen primary issues were determined. These issues can be grouped into those that can be determined by documentation of available information and present conditions, those that require extensive field investigations and those that require some form of predictive modeling. To address the various issues requires an integrated effort of hydrogeologists, environmental engineers or scientists and health physicists. In this paper, the approach to the resolution of these fourteen issues is described briefly

  6. Characterizing chemical systems with on-line computers and graphics

    Frazer, J.W.; Rigdon, L.P.; Brand, H.R.; Pomernacki, C.L.

    1979-01-01

    Incorporating computers and graphics on-line to chemical experiments and processes opens up new opportunities for the study and control of complex systems. Systems having many variables can be characterized even when the variable interactions are nonlinear, and the system cannot a priori be represented by numerical methods and models. That is, large sets of accurate data can be rapidly acquired, then modeling and graphic techniques can be used to obtain partial interpretation plus design of further experimentation. The experimenter can thus comparatively quickly iterate between experimentation and modeling to obtain a final solution. We have designed and characterized a versatile computer-controlled apparatus for chemical research, which incorporates on-line instrumentation and graphics. It can be used to determine the mechanism of enzyme-induced reactions or to optimize analytical methods. The apparatus can also be operated as a pilot plant to design control strategies. On-line graphics were used to display conventional plots used by biochemists and three-dimensional response-surface plots

  7. Establishment and characterization of a novel osteosarcoma cell line: CHOS.

    Liu, Yunlu; Feng, Xiaobo; Zhang, Yukun; Jiang, Hongyan; Cai, Xianyi; Yan, Xinxin; Huang, Zengfa; Mo, Fengbo; Yang, Wen; Yang, Cao; Yang, Shuhua; Liu, Xianzhe

    2016-12-01

    Osteosarcoma has a well-recognized bimodal distribution, with the first peak in adolescence and another in the elderly age-group. The elderly patients have different clinical features and a poorer prognosis as compared to adolescents. To better understand the biological features of osteosarcoma in the elderly population, we established a new human osteosarcoma cell line from a 58-year-old man with primary chondroblastic osteosarcoma. After 6 months of continuous culture in vitro for over 50 passages, an immortalized cell line CHOS was established. The cell line was well-characterized by cytogenetic, biomarker, functional, and histological analyses. The CHOS cells exhibited a spindle-shaped morphology and a doubling time of 36 h. Cytogenetic analysis of CHOS cells revealed the loss of chromosome Y and the gain of chromosome 12. Quantitative real-time polymerase chain reaction (RT-PCR), Western blotting and/or immunofluorescence revealed the expression of chondroblastic, mesenchymal and tumor metastasis markers in the CHOS cells. Compared with the osteosarcoma cell line, the CHOS cells were found to be more sensitive to cisplatin and doxorubicin, but were resistant to methotrexate. The cell line was highly tumorigenic and maintained the histological characteristics and invasive nature of the original tumor. Furthermore, on immunohistochemical analysis, the xenografts and metastases were found to co-express collagen II, aggrecan, vimentin and S100A4 that resembled the original tumor cells. Our results indicate, the potential of CHOS cell line to serve as a useful tool for further studies on the molecular biology of osteosarcoma, especially in the elderly patients. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:2116-2125, 2016. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

  8. Characterizing Exoplanet Atmospheres : A Complete Line List for Phosphine

    Sousa-Silva, C.; Yurchenko, S. N.; Tennyson, J.

    2013-09-01

    The ability to characterise the atmospheres of cool stars, brown dwarfs and exoplanets requires fundamental data for all species contributing significantly to their opacity. However, with notable exceptions such as water and ammonia, existing molecular line lists are not sufficiently accurate or complete to allow for a full spectroscopic analysis of these bodies. ExoMol (www.exomol.com [1]) is a project that aims to rectify this by generating comprehensive line lists for all molecules likely to be detected in the atmospheres of cool astrophysical objects in the foreseeable future. The spectral data is generated by employing ab initio quantum mechanical methods, performing empirical refinement based on experimental spectroscopic data and harnessing high performance computing. Here we present our work on phosphine, (PH3), an equilateral pyramidal molecule (the phosphorus analogue to ammonia). Phosphine is known to be important for the atmospheres of giant-planets, cool stars and many other astronomical bodies. Rotational transition features of phosphine have been found in the far- infrared spectra of Saturn and Jupiter [2, 3], where it is a marker for vertical convection zones. A computed room temperature line list of phosphine is presented here [4], illustrated in the accompanying figure 1. This line list is a precursor to a high temperature equivalent to be produced in the near future, necessary for the analysis of cool stars and brown dwarfs. All the transitions' energy levels and Einstein A-coefficients were computed using the program TROVE [5].

  9. Yucca Mountain Site characterization project bibliography, January--June 1991

    1992-06-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy's Science and Technology Database from January 1, 1990, through December 31, 1991

  10. Cellular and Phenotypic Characterization of Canine Osteosarcoma Cell Lines

    Marie E. Legare, Jamie Bush, Amanda K. Ashley, Taka Kato, William H. Hanneman

    2011-01-01

    Full Text Available Canine and human osteosarcoma (OSA have many similarities, with the majority of reported cases occurring in the appendicular skeleton, gender predominance noted, high rate of metastasis at the time of presentation, and a lack of known etiology for this devastating disease. Due to poor understanding of the molecular mechanisms underlying OSA, we have characterized seven different OSA canine cell lines: Abrams, D17, Grey, Hughes, Ingles, Jarques, and Marisco and compared them to U2, a human OSA cell line, for the following parameters: morphology, growth, contact inhibition, migrational tendencies, alkaline phosphatase staining, heterologous tumor growth, double-strand DNA breaks, and oxidative damage. All results demonstrated the positive characteristics of the Abrams cell line for use in future studies of OSA. Of particular interest, the robust growth of a subcutaneous tumor and rapid pulmonary metastasis of the Abrams cell line in an immunocompromised mouse shows incredible potential for the future use of Abrams as a canine OSA model. Further investigations utilizing a canine cell model of OSA, such as Abrams, will be invaluable to understanding the molecular events underlying OSA, pharmaceutical inhibition of metastasis, and eventual prevention of this devastating disease.

  11. Size characterization of airborne SiO2 nanoparticles with on-line and off-line measurement techniques: an interlaboratory comparison study

    Motzkus, C.; Macé, T.; Gaie-Levrel, F.; Ducourtieux, S.; Delvallee, A.; Dirscherl, K.; Hodoroaba, V.-D.; Popov, I.; Popov, O.; Kuselman, I.; Takahata, K.; Ehara, K.; Ausset, P.; Maillé, M.; Michielsen, N.; Bondiguel, S.; Gensdarmes, F.; Morawska, L.; Johnson, G. R.; Faghihi, E. M.; Kim, C. S.; Kim, Y. H.; Chu, M. C.; Guardado, J. A.; Salas, A.; Capannelli, G.; Costa, C.; Bostrom, T.; Jämting, Å. K.; Lawn, M. A.; Adlem, L.; Vaslin-Reimann, S.

    2013-10-01

    Results of an interlaboratory comparison on size characterization of SiO2 airborne nanoparticles using on-line and off-line measurement techniques are discussed. This study was performed in the framework of Technical Working Area (TWA) 34—"Properties of Nanoparticle Populations" of the Versailles Project on Advanced Materials and Standards (VAMAS) in the project no. 3 "Techniques for characterizing size distribution of airborne nanoparticles". Two types of nano-aerosols, consisting of (1) one population of nanoparticles with a mean diameter between 30.3 and 39.0 nm and (2) two populations of non-agglomerated nanoparticles with mean diameters between, respectively, 36.2-46.6 nm and 80.2-89.8 nm, were generated for characterization measurements. Scanning mobility particle size spectrometers (SMPS) were used for on-line measurements of size distributions of the produced nano-aerosols. Transmission electron microscopy, scanning electron microscopy, and atomic force microscopy were used as off-line measurement techniques for nanoparticles characterization. Samples were deposited on appropriate supports such as grids, filters, and mica plates by electrostatic precipitation and a filtration technique using SMPS controlled generation upstream. The results of the main size distribution parameters (mean and mode diameters), obtained from several laboratories, were compared based on metrological approaches including metrological traceability, calibration, and evaluation of the measurement uncertainty. Internationally harmonized measurement procedures for airborne SiO2 nanoparticles characterization are proposed.

  12. Size characterization of airborne SiO{sub 2} nanoparticles with on-line and off-line measurement techniques: an interlaboratory comparison study

    Motzkus, C., E-mail: charles.motzkus@lne.fr; Mace, T.; Gaie-Levrel, F.; Ducourtieux, S.; Delvallee, A. [Laboratoire National de Metrologie et d' Essais (LNE) (France); Dirscherl, K. [Danish Fundamental Metrology (DFM) (Denmark); Hodoroaba, V.-D. [BAM Federal Institute for Materials Research and Testing (Germany); Popov, I. [The Hebrew University of Jerusalem, Unit for Nanocharacterization (Israel); Popov, O.; Kuselman, I. [National Physical Laboratory of Israel (INPL) (Israel); Takahata, K.; Ehara, K. [National Institute of Advanced Industrial Science and Technology (AIST), National Metrology Institute of Japan (NMIJ) (Japan); Ausset, P.; Maille, M. [Universite Paris-Est Creteil et Universite Paris-Diderot, Laboratoire Interuniversitaire des Systemes Atmospheriques (LISA), UMR CNRS 7583 (France); Michielsen, N.; Bondiguel, S.; Gensdarmes, F. [Institut de Radioprotection et de Surete Nucleaire (IRSN), PSN-RES, SCA, LPMA (France); Morawska, L.; Johnson, G. R.; Faghihi, E. M. [Queensland University of Technology (QUT), International Laboratory for Air Quality and Health (ILAQH) (Australia); and others

    2013-10-15

    Results of an interlaboratory comparison on size characterization of SiO{sub 2} airborne nanoparticles using on-line and off-line measurement techniques are discussed. This study was performed in the framework of Technical Working Area (TWA) 34-'Properties of Nanoparticle Populations' of the Versailles Project on Advanced Materials and Standards (VAMAS) in the project no. 3 'Techniques for characterizing size distribution of airborne nanoparticles'. Two types of nano-aerosols, consisting of (1) one population of nanoparticles with a mean diameter between 30.3 and 39.0 nm and (2) two populations of non-agglomerated nanoparticles with mean diameters between, respectively, 36.2-46.6 nm and 80.2-89.8 nm, were generated for characterization measurements. Scanning mobility particle size spectrometers (SMPS) were used for on-line measurements of size distributions of the produced nano-aerosols. Transmission electron microscopy, scanning electron microscopy, and atomic force microscopy were used as off-line measurement techniques for nanoparticles characterization. Samples were deposited on appropriate supports such as grids, filters, and mica plates by electrostatic precipitation and a filtration technique using SMPS controlled generation upstream. The results of the main size distribution parameters (mean and mode diameters), obtained from several laboratories, were compared based on metrological approaches including metrological traceability, calibration, and evaluation of the measurement uncertainty. Internationally harmonized measurement procedures for airborne SiO{sub 2} nanoparticles characterization are proposed.

  13. Size characterization of airborne SiO2 nanoparticles with on-line and off-line measurement techniques: an interlaboratory comparison study

    Motzkus, C.; Macé, T.; Gaie-Levrel, F.; Ducourtieux, S.; Delvallee, A.; Dirscherl, K.; Hodoroaba, V.-D.; Popov, I.; Popov, O.; Kuselman, I.; Takahata, K.; Ehara, K.; Ausset, P.; Maillé, M.; Michielsen, N.; Bondiguel, S.; Gensdarmes, F.; Morawska, L.; Johnson, G. R.; Faghihi, E. M.

    2013-01-01

    Results of an interlaboratory comparison on size characterization of SiO 2 airborne nanoparticles using on-line and off-line measurement techniques are discussed. This study was performed in the framework of Technical Working Area (TWA) 34—“Properties of Nanoparticle Populations” of the Versailles Project on Advanced Materials and Standards (VAMAS) in the project no. 3 “Techniques for characterizing size distribution of airborne nanoparticles”. Two types of nano-aerosols, consisting of (1) one population of nanoparticles with a mean diameter between 30.3 and 39.0 nm and (2) two populations of non-agglomerated nanoparticles with mean diameters between, respectively, 36.2–46.6 nm and 80.2–89.8 nm, were generated for characterization measurements. Scanning mobility particle size spectrometers (SMPS) were used for on-line measurements of size distributions of the produced nano-aerosols. Transmission electron microscopy, scanning electron microscopy, and atomic force microscopy were used as off-line measurement techniques for nanoparticles characterization. Samples were deposited on appropriate supports such as grids, filters, and mica plates by electrostatic precipitation and a filtration technique using SMPS controlled generation upstream. The results of the main size distribution parameters (mean and mode diameters), obtained from several laboratories, were compared based on metrological approaches including metrological traceability, calibration, and evaluation of the measurement uncertainty. Internationally harmonized measurement procedures for airborne SiO 2 nanoparticles characterization are proposed

  14. Generation and Characterization of an Immortalized Human Esophageal Myofibroblast Line.

    Chao Niu

    Full Text Available Stromal cells with a myofibroblast phenotype present in the normal human esophagus are increased in individuals with gastro-esophageal reflux disease (GERD. We have previously demonstrated that myofibroblasts stimulated with acid and TLR4 agonists increase IL-6 and IL-8 secretion using primary cultures of myofibroblasts established from normal human esophagus. While primary cultures have the advantage of reflecting the in vivo environment, a short life span and unavoidable heterogeneity limits the usefulness of this model in larger scale in vitro cellular signaling studies. The major aim of this paper therefore was to generate a human esophageal myofibroblast line with an extended lifespan. In the work presented here we have generated and characterized an immortalized human esophageal myofibroblast line by transfection with a commercially available GFP-hTERT lentivirus. Immortalized human esophageal myofibroblasts demonstrate phenotypic, genotypic and functional similarity to primary cultures of esophageal myofibroblasts we have previously described. We found that immortalized esophageal myofibroblasts retain myofibroblast spindle-shaped morphology at low and high confluence beyond passage 80, and express α-SMA, vimentin, and CD90 myofibroblast markers. Immortalized human esophageal myofibroblasts also express the putative acid receptor TRPV1 and TLR4 and retain the functional capacity to respond to stimuli encountered in GERD with secretion of IL-6. Finally, immortalized human esophageal myofibroblasts also support the stratified growth of squamous esophageal epithelial cells in 3D organotypic cultures. This newly characterized immortalized human esophageal myofibroblast cell line can be used in future cellular signaling and co-culture studies.

  15. Coronal Physics and the Chandra Emission Line Project

    Brickhouse, N. S.; Drake, J. J.

    2000-01-01

    With the launch of the Chandra X-ray Observatory, high resolution X-ray spectroscopy of cosmic sources has begun. Early, deep observations of three stellar coronal sources Capella, Procyon, and HR 1099 are providing not only invaluable calibration data, but also benchmarks for plasma spectral models. These models are needed to interpret data from stellar coronae, galaxies and clusters of galaxies, supernova, remnants and other astrophysical sources. They have been called into question in recent years as problems with understanding low resolution ASCA and moderate resolution Extreme Ultraviolet Explorer Satellite (EUVE) data have arisen. The Emission Line Project is a collaborative effort, to improve the models, with Phase I being the comparison of models with observed spectra of Capella, Procyon, and HR 1099. Goals of these comparisons are (1) to determine and verify accurate and robust diagnostics and (2) to identify and prioritize issues in fundamental spectroscopy which will require further theoretical and/or laboratory work. A critical issue in exploiting the coronal data for these purposes is to understand the extent, to which common simplifying assumptions (coronal equilibrium, negligible optical depth) apply. We will discuss recent, advances in our understanding of stellar coronae, in this context.

  16. Site characterization activities at Stripa and other Swedish projects

    Ahlstroehm, P.E.

    1991-01-01

    The Swedish research programme concerning spent nuclear fuel disposal aims for submitting a siting license application around the year 2000. An important step towards that goal will be the detailed characterization of at least two potential sites in late 1990s. In preparation for such characterization several research projects are conducted. One is the international Stripa Project that includes a site characterization and validation project for a small size granite rock body. The Stripa work also includes further development of instrumentation and measurement techniques. Another project is the Finnsjoen Fracture Zone Project, which is characterizing a subhorizontal zone at depths from 100 to 350 meters. The third project is the new Swedish Hard Rock Laboratory planned at the site of the Oskarshamn nuclear power plant. The preinvestigations and construction of this laboratory include major efforts in development, application and validation of site characterization methodology. (author) 6 figs., 9 refs

  17. Molecular cytogenetic characterization of a human thyroid cancercell line

    Weier, Heinz-Ulrich G.; Tuton, Tiffany B.; Ito, Yuko; Chu, LisaW.; Lu, Chung-Mei; Baumgartner, Adolf; Zitzelsberger, Horst F.; Weier,Jingly F.

    2006-01-04

    The incidence of papillary thyroid carcinoma (PTC) increases significantly after exposure of the head and neck region to ionizing radiation, yet we know neither the steps involved in malignant transformation of thyroid epithelium nor the specific carcinogenic mode of action of radiation. Such increased tumor frequency became most evident in children after the 1986 nuclear accident in Chernobyl, Ukraine. In the twelve years following the accident, the average incidence of childhood PTCs (chPTC) increased over one hundred-fold compared to the rate of about 1 tumor incidence per 10{sup 6} children per year prior to 1986. To study the etiology of radiation-induced thyroid cancer, we formed an international consortium to investigate chromosomal changes and altered gene expression in cases of post-Chernobyl chPTC. Our approach is based on karyotyping of primary cultures established from chPTC specimens, establishment of cell lines and studies of genotype-phenotype relationships through high resolution chromosome analysis, DNA/cDNA micro-array studies, and mouse xenografts that test for tumorigenicity. Here, we report the application of fluorescence in situ hybridization (FISH)-based techniques for the molecular cytogenetic characterization of a highly tumorigenic chPTC cell line, S48TK, and its subclones. Using chromosome 9 rearrangements as an example, we describe a new approach termed ''BAC-FISH'' to rapidly delineate chromosomal breakpoints, an important step towards a better understanding of the formation of translocations and their functional consequences.

  18. Tank Waste Remediation System Characterization Project Programmatic Risk Management Plan

    Baide, D.G.; Webster, T.L.

    1995-12-01

    The TWRS Characterization Project has developed a process and plan in order to identify, manage and control the risks associated with tank waste characterization activities. The result of implementing this process is a defined list of programmatic risks (i.e. a risk management list) that are used by the Project as management tool. This concept of risk management process is a commonly used systems engineering approach which is being applied to all TWRS program and project elements. The Characterization Project risk management plan and list are subset of the overall TWRS risk management plan and list

  19. Defining project scenarios for the agile requirements engineering product-line development questionnaire

    Feng, Kunwu; Lempert, Meli; Tang, Yan; Tian, Kun; Cooper, Kendra M.L.; Franch Gutiérrez, Javier

    2007-01-01

    Current agile methods are focused on practices of small, rapid developing and iteration, more people oriented, less documentation projects, and the use of the methods in large, product line projects are somehow difficult. UTD and GESSI have started a project to develop an expert system that can assist a requirements enginer in selecting a requirements engineering process that is well suited for their project, in particular with respect to the use of agile and product line engineering methods....

  20. TWRS phase 1 infrastructure project (W-519) characterization

    Mitchell, C.J.

    1998-01-01

    In order to treat the mixed radioactive and hazardous waste stored in 177 underground tanks, the Tank Waste Remediation System (TWRS) program is developing a 'demonstration' site for treatment and immobilization of these wastes by a private contractor. Project W-519 is providing the infrastructure support to this site by developing the designs and emplacing required pipelines, roads, electrical, etc. In support of the TWRS Phase 1 Infrastructure Project (W-519) Characterization, Numatec Hanford Corporation (NHC) contracted with Waste Management Federal Services, Inc., Northwest Operations (WMNW) to investigate a number of locations in and just outside the 200 East Area eastern fenceline boundary. These areas consisted of known or suspected waste lines or waste sites that could potentially impact the construction and emplacement of the proposed facility improvements, including waterlines and roads. These sites were all located subsurface and sugaring would be required to obtain sample material from the desired depth. The soils would then be sampled and submitted to the laboratory for analysis of radioactivity

  1. Economic Impact Analysis of Short Line Railroads : Research Project Capsule

    2012-10-01

    Rail transportation is of vital : importance to the state of : Louisiana and is a key : component of the states : business infrastructure, supporting agricultural, petroleum, chemical, and manufacturing : industries across the state. Short line (C...

  2. Yucca Mountain Site characterization project bibliography, January--June 1991

    Lorenz, J.J.; Stephan, P.M.

    1991-09-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy's Energy Science and Technology Database from January 1991 through June 1991. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers, and articles are included in the sponsoring organization's list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it

  3. Yucca Mountain Site Characterization Project bibliography, January--June 1992

    1992-01-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy's Energy Science and Technology Database from January 1, 1993, through June 30, 1993. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers, and articles are included in the sponsoring organization's list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it

  4. Yucca Mountain Site Characterization Project Bibliography, July--December 1990

    1991-05-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountains Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy's Energy Science and Technology Database from July 1990 through December 1990. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers and articles are included in the sponsoring organizations list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it

  5. Yucca Mountain Site characterization project bibliography, January--June 1992

    1992-09-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy's Energy Science and Technology Database from January 1, 1992, through June 30, 1992. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor resorts, papers, and articles are included in the sponsoring organization's list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it

  6. Characterization of selection effects on broiler lines using DNA fingerprinting

    GS Schmidt

    2003-05-01

    Full Text Available The objective of this study was to evaluate the effect of selection for body weight on the genetic variability and diversity in broiler lines. Two paternal broiler lines (LL and LLc were used. LL line was selected for 12 generations for growth and carcass and reproduction characteristics. The LLc line was established from LL line in 1985 and mated at random. Blood samples from six chickens per line were collected and used for molecular analysis. Also, a DNA pool was made for each line to compare effects between lines. Data were analyzed considering the collected information on the presence or absence of DNA bands. Band sharing scores were calculated using the DICE coefficient. The pattern of the 21 most representative bands was used. DNA fingerprinting (DFP showed 90.48 % of polymorphism bands for both lines. Difference between lines was not due to the presence or absence of bands, but to the frequency of such bands in each genotype. Considering that both lines had the same genetic background, changes on band frequency were probably due to selection. Selection for body weight had an effect on the band frequency as evaluated by DFP, and for this reason this technique could be used as a tool in the selection process. Results also suggest that bands 4, 5 and 19 were linked to body weight traits, and bands 9, 10, 12, 13 and 21 were linked to reproductive traits such as egg production.

  7. Area 5 Site characterization project report, FY 1994

    Albright, W.; Tyler, S.; Chapman, J.; Miller, M.; Estrella, R.

    1994-09-01

    The Area 5 Site Characterization Project is designed to determine the suitability of the Radioactive Waste Management Site (RWMS) for disposal of low-level waste (LLW), mixed waste (MW) and transuranic waste (TRU). The Desert Research institute (DRI) has conducted this study for the Area 5 Site Characterization Project for the US Department of Energy, Nevada Operations Office (DOE/NV), Waste Management Division (WMD). The purpose of DRI's Area 5 Site Characterization Project is to characterize important properties of the upper vadose zone which influence infiltration and redistribution of water and transport of solutes as well as to characterize the water quality and hydrologic conditions of the uppermost aquifer. This report describes methods and presents a summary of all data and results from laboratory physical and chemical testing from borehole samples through September 1994. DRI laboratories performed soil water content, soil water potential, soil bulk density, and soil water extract isotope analyses

  8. Software quality assurance on the Yucca Mountain Site Characterization Project

    Matras, J.R.

    1993-01-01

    The Yucca Mountain Site Characterization Project (YMP) has been involved over the years in the continuing struggle with establishing acceptable Software Quality Assurance (SQA) requirements for the development, modification, and acquisition of computer programs used to support the Mined Geologic Disposal System. These computer programs will be used to produce or manipulate data used directly in site characterization, design, analysis, performance assessment, and operation of repository structures, systems, and components. Scientists and engineers working on the project have claimed that the SQA requirements adopted by the project are too restrictive to allow them to perform their work. This paper will identify the source of the original SQA requirements adopted by the project. It will delineate the approach used by the project to identify concerns voiced by project engineers and scientists regarding the original SQA requirements. It will conclude with a discussion of methods used to address these problems in the rewrite of the original SQA requirements

  9. Characterizations of p-Wavelets on Positive Half Line Using the Walsh-Fourier Transform

    Abdullah Abdullah

    2016-03-01

    Full Text Available In this paper, we study the characterization of wavelets on positive half line by means of two basic equations in the Fourier domain. We also give another characterization of wavelets.

  10. Hot-Gas Filter Ash Characterization Project

    Swanson, M.L.; Hurley, J.P.; Dockter, B.A.; O`Keefe, C.A.

    1997-07-01

    Large-scale hot-gas filter testing over the past 10 years has revealed numerous cases of cake buildup on filter elements that has been difficult, if not impossible, to remove. At times, the cake can blind or bridge between candle filters, leading to filter failure. Physical factors, including particle-size distribution, particle shape, the aerodynamics of deposition, and system temperature, contribute to the difficulty in removing the cake, but chemical factors such as surface composition and gas-solid reactions also play roles in helping to bond the ash to the filters or to itself. This project is designed to perform the research necessary to determine the fuel-, sorbent-, and operations-related conditions that lead to blinding or bridging of hot-gas particle filters. The objectives of the project are threefold: (1) Determine the mechanisms by which a difficult-to-clean ash is formed and how it bridges hot-gas filters (2) Develop a method to determine the rate of bridging based on analyses of the feed coal and sorbent, filter properties, and system operating conditions and (3) Suggest and test ways to prevent filter bridging.

  11. Teaching Project Management On-Line: Lessons Learned from MOOCs

    Falcao, Rita; Fernandes, Luis

    2016-01-01

    Creating a course for teaching project management online in a full online distance-learning environment was a challenge. Working with adult learners from different continents that want to complete a Master degree was an additional challenge. This paper describes how different MOOCs were used to learn about teaching -(meta) e-learning. MOOCs…

  12. Characterization and performance of 16 new inbred lines of lettuce

    The Agricultural Research Service, U.S. Department of Agriculture announces the release of sixteen breeding lines of lettuce (Lactuca sativa L.). Five (SM13-I1, SM13-I2, SM13-I3, SM13-I4, and SM13-I5) of the six iceberg breeding lines can be used for whole head or salad blend production; the sixth i...

  13. Characterization of the Goubau line for testing beam diagnostic instruments

    Kim, S. Y.; Stulle, F.; Sung, C. K.; Yoo, K. H.; Seok, J.; Moon, K. J.; Choi, C. U.; Chung, Y.; Kim, G.; Woo, H. J.; Kwon, J.; Lee, I. G.; Choi, E. M.; Chung, M.

    2017-12-01

    One of the main characteristics of the Goubau line is that it supports a low-loss, non-radiated surface wave guided by a dielectric-coated metal wire. The dominant mode of the surface wave along the Goubau line is a TM01 mode, which resembles the pattern of the electromagnetic fields induced in the metallic beam pipe when the charged particle beam passes through it. Therefore, the Goubau line can be used for the preliminary bench test and performance optimization of the beam diagnostic instruments without requiring charged particle beams from the accelerators. In this paper, we discuss the basic properties of the Goubau line for testing beam diagnostic instruments and present the initial test results for button-type beam position monitors (BPMs). The experimental results are consistent with the theoretical estimations, which indicates that Goubau line allows effective testing of beam diagnostic equipment.

  14. Spent nuclear fuels project characterization data quality objectives strategy

    Lawrence, L.A.; Thornton, T.A.; Redus, K.S.

    1994-12-01

    A strategy is presented for implementation of the Data Quality Objectives (DQO) process to the Spent Nuclear Fuels Project (SNFP) characterization activities. Westinghouse Hanford Company (WHC) and the Pacific Northwest Laboratory (PNL) are teaming in the characterization of the SNF on the Hanford Site and are committed to the DQO process outlined in this strategy. The SNFP characterization activities will collect and evaluate the required data to support project initiatives and decisions related to interim safe storage and the path forward for disposal. The DQO process is the basis for the activity specific SNF characterization requirements, termed the SNF Characterization DQO for that specific activity, which will be issued by the WHC or PNL organization responsible for the specific activity. The Characterization Plan prepared by PNL defines safety, remediation, and disposal issues. The ongoing Defense Nuclear Facility Safety Board (DNFSB) requirement and plans and the fuel storage and disposition options studies provide the need and direction for the activity specific DQO process. The hierarchy of characterization and DQO related documentation requirements is presented in this strategy. The management of the DQO process and the means of documenting the DQO process are described as well as the tailoring of the DQO process to the specific need of the SNFP characterization activities. This strategy will assure stakeholder and project management that the proper data was collected and evaluated to support programmatic decisions

  15. Reasons for decision in the matter of Enbridge Pipelines Inc. Line 4 Extension Project : facilities[Application dated 28 June 2007 for the Line 4 Extension Project

    NONE

    2008-04-15

    In June 2007, Enbridge Pipelines Inc. applied for approval to extend Line 4 of its Mainline pipeline upstream from Hardisty to Edmonton, Alberta. The project would relieve a potential bottleneck and would also add an additional line across this segment to increase system security and flexibility. The average annual capacity of Line 4 would be 140,000 cubic metres per day. The Enbridge Mainline from Edmonton to Hardisty currently includes 3 oil pipelines, while the Enbridge Mainline downstream of Hardisty includes 4 oil pipelines. This document presented the views of the Board regarding the construction and operation of the project facilities; public and Aboriginal consultation; environmental and socio-economic matters; land matters; tolls and tariffs; and, supply, markets, financing and economics. The Board noted that the benefits of this project outweigh the burdens associated with it. The project required an environmental assessment under the Canadian Environmental Assessment Act which found that it is not likely to cause significant adverse environmental effects. In its approval of the project, the Board attached 15 conditions, including a requirement for Enbridge to file an updated Environmental Protection Plan for the Board's approval. The Board also approved Enbridge's application for its tolling method and to reactivate 3 sections of pre-existing pipeline. 3 tabs., 3 figs., 3 appendices.

  16. Projection methods for line radiative transfer in spherical media.

    Anusha, L. S.; Nagendra, K. N.

    An efficient numerical method called the Preconditioned Bi-Conjugate Gradient (Pre-BiCG) method is presented for the solution of radiative transfer equation in spherical geometry. A variant of this method called Stabilized Preconditioned Bi-Conjugate Gradient (Pre-BiCG-STAB) is also presented. These methods are based on projections on the subspaces of the n dimensional Euclidean space mathbb {R}n called Krylov subspaces. The methods are shown to be faster in terms of convergence rate compared to the contemporary iterative methods such as Jacobi, Gauss-Seidel and Successive Over Relaxation (SOR).

  17. Derivation and characterization of a pig embryonic stem cell-derived exocrine pancreatic cell line

    The establishment and initial characterization of a pig embryonic stem cell-derived pancreatic cell line, PICM-31, and a colony-cloned derivative cell line, PICM-31A, is described. The cell lines were propagated for several months at split ratios of 1:3 or 1:5 at each passage on STO feeder cells af...

  18. The 12th 735-kV transmission line project: Hydro-Quebec network

    1992-01-01

    Hydro-Quebec is proposing to construct a 735-kV transmission line from the Chissibi station near the LG3 hydroelectric power plant to the Jacques-Cartier station near Quebec City. The width of the right-of-way will generally be 90 m and it is intended to install the line in two stages. The Chissibi-Chibougamau section should enter service in 1993 and the Chibougamau-Cartier section in 1994. The cost of the line is estimated at $1.21 billion, of which $900 million is for line construction and $310 million for station equipment. Prospective corridors for the line route have been selected, portions of which enable twinning with Hydro-Quebec's existing 9th 735-kV line. A series of hearings on the environmental and other impacts of the power line project was held by the Bureau d'audiences publiques sur l'environnement and results are summarized along with the views of the Bureau. The summary describes the justification for the project, the choice of the transmission line route, the evaluation of the impacts of the project on the natural and human environments, health effects due to magnetic fields, and effects on the economic development of northern Quebec. Recommendations are given for mitigating the impacts of the project. 13 refs., 8 figs., 2 tabs

  19. Spherical Projection Based Straight Line Segment Extraction for Single Station Terrestrial Laser Point Cloud

    ZHANG Fan

    2015-06-01

    Full Text Available Due to the discrete distribution computing errors and lack of adaptability are ubiquitous in the current straight line extraction for TLS data methods. A 3D straight line segment extraction method is proposed based on spherical projection for single station terrestrial laser point clouds. Firstly, horizontal and vertical angles of each laser point are calculated by means of spherical coordinates, intensity panoramic image according to the two angles is generated. Secondly, edges which include straight line features are detected from intensity panoramic image by using of edge detection algorithm. Thirdly, great circles are detected from edges of panoramic image using spherical Hough transform. According to the axiom that a straight line segment in 3D space is a spherical great circle after spherical projection, detecting great circles from spherical projected data sets is essentially detecting straight line segments from 3D data sets without spherical projection. Finally, a robust 3D straight line fitting method is employed to fitting the straight lines and calculating parameters of the straight line segments. Experiments using different data sets and comparison with other methods show the accuracy and applicability of the proposed method.

  20. A 110 m long prototype helium transfer line for the HERA project

    Horvath, I.; Ming, P.; Von Burg, M.; Horlitz, G.; Sindt, H.

    1986-01-01

    A prototype helium transfer line of 110-m length was designed and constructed at SIN for the HERA project. This rendered a study of the assembly and handling processes as well as the determination of the heat losses into the transfer line. In this paper the transfer line structure is described and first results of heat transfer measurement for the specially developed super-insulation are presented

  1. LINES

    Minas Bakalchev

    2015-10-01

    Full Text Available The perception of elements in a system often creates their interdependence, interconditionality, and suppression. The lines from a basic geometrical element have become the model of a reductive world based on isolation according to certain criteria such as function, structure, and social organization. Their traces are experienced in the contemporary world as fragments or ruins of a system of domination of an assumed hierarchical unity. How can one release oneself from such dependence or determinism? How can the lines become less “systematic” and forms more autonomous, and less reductive? How is a form released from modernistic determinism on the new controversial ground? How can these elements or forms of representation become forms of action in the present complex world? In this paper, the meaning of lines through the ideas of Le Corbusier, Leonidov, Picasso, and Hitchcock is presented. Spatial research was made through a series of examples arising from the projects of the architectural studio “Residential Transformations”, which was a backbone for mapping the possibilities ranging from playfulness to exactness, as tactics of transformation in the different contexts of the contemporary world.

  2. Workshop on a project for a FZR-beam line at ESRF

    Matz, W.

    1993-10-01

    The Research Center Rossendorf (FZR) investigates the possibilities to install its own beam line as a Cooperate Research Group-project (CRG) at the European Synchrotron Radiation Facility (ESRF) in Grenoble. The main interests for the FZR to use high brillant synchrotron radiation are in the Institute of Radiochemistry and the Institute of Ion Beam Physics and Materials Research. This workshop was organized by these two institutes together with the FZR Study group Synchrotron. The purpose of the workshop was to achieve a better understanding for the technical needs of the projected beam line for the planned research projects. Experts with experience in beam line design met with the Rossendorf groups to discuss the best layout for such a beam line. The summary of this workshop and the copies of transparencies of the lectures that were given are published in this booklet. (orig.)

  3. Rhumb lines and map wars a social history of the Mercator projection

    Monmonier, Mark

    2010-01-01

    In Rhumb Lines and Map Wars, Mark Monmonier offers an insightful, richly illustrated account of the controversies surrounding Flemish cartographer Gerard Mercator's legacy. He takes us back to 1569, when Mercator announced a clever method of portraying the earth on a flat surface, creating the first projection to take into account the earth's roundness. As Monmonier shows, mariners benefited most from Mercator's projection, which allowed for easy navigation of the high seas with rhumb lines-clear-cut routes with a constant compass bearing-for true direction. But the projection's popularity amo

  4. In-line quantification and characterization of membrane fouling

    Bucs, Szilard; Hekkert, Sacco Te Lintel; Staal, Marc Jaap; Vrouwenvelder, Johannes S.

    2016-01-01

    Methods of detecting, quantifying and/or characterizing the fouling of a device from a combination of pressure and spectroscopic data are provided. The device can be any device containing components susceptible to fouling. Components can include

  5. Characterization of a high-energy in-line phase contrast tomosynthesis prototype.

    Wu, Di; Yan, Aimin; Li, Yuhua; Wong, Molly D; Zheng, Bin; Wu, Xizeng; Liu, Hong

    2015-05-01

    In this research, a high-energy in-line phase contrast tomosynthesis prototype was developed and characterized through quantitative investigations and phantom studies. The prototype system consists of an x-ray source, a motorized rotation stage, and a CMOS detector with a pixel pitch of 0.05 mm. The x-ray source was operated at 120 kVp for this study, and the objects were mounted on the rotation stage 76.2 cm (R1) from the source and 114.3 cm (R2) from the detector. The large air gap between the object and detector guarantees sufficient phase-shift effects. The quantitative evaluation of this prototype included modulation transfer function and noise power spectrum measurements conducted under both projection mode and tomosynthesis mode. Phantom studies were performed including three custom designed phantoms with complex structures: a five-layer bubble wrap phantom, a fishbone phantom, and a chicken breast phantom with embedded fibrils and mass structures extracted from an ACR phantom. In-plane images of the phantoms were acquired to investigate their image qualities through observation, intensity profile plots, edge enhancement evaluations, and/or contrast-to-noise ratio calculations. In addition, the robust phase-attenuation duality (PAD)-based phase retrieval method was applied to tomosynthesis for the first time in this research. It was utilized as a preprocessing method to fully exhibit phase contrast on the angular projection before reconstruction. The resolution and noise characteristics of this high-energy in-line phase contrast tomosynthesis prototype were successfully investigated and demonstrated. The phantom studies demonstrated that this imaging prototype can successfully remove the structure overlapping in phantom projections, obtain delineate interfaces, and achieve better contrast-to-noise ratio after applying phase retrieval to the angular projections. This research successfully demonstrated a high-energy in-line phase contrast tomosynthesis

  6. In-line quantification and characterization of membrane fouling

    Bucs, Szilard

    2016-06-16

    Methods of detecting, quantifying and/or characterizing the fouling of a device from a combination of pressure and spectroscopic data are provided. The device can be any device containing components susceptible to fouling. Components can include membranes, pipes, or reactors. Suitable devices include membrane devices, heat exchangers, and chemical or bio-reactors. Membrane devices can include, for example, microfiltration devices, ultrafiltration devices, nanofiltration devices, reverse osmosis, forward osmosis, osmosis, reverse electrodialysis, electro- deionisation or membrane distillation devices. The methods can be applied to any type of membrane, including tubular, spiral, hollow fiber, flat sheet, and capillary membranes. The spectroscopic characterization can include measuring one or more of the absorption, fluorescence, or raman spectroscopic data of one or more foulants. The methods can allow for the early detection and/or characterization of fouling. The characterization can include determining the specific foulant(s) or type of foulant(s) present. The characterization of fouling can allow for the selection of an appropriate de-fouling method and timing.

  7. Tank waste remediation system characterization project quality policies

    Board, D.C.

    1997-01-01

    This quality plan describes the system used by Characterization Project management to achieve quality. This plan is comprised of eleven quality policies which, when taken together, form a management system deployed to achieve quality. This quality management system is based on the customer's quality requirements known as the 'RULE', 10 CFR 830.120, Quality Assurance

  8. Characterization and evaluation of rice blast resistance of Chinese indica hybrid rice parental lines

    Yunyu Wu

    2017-12-01

    Full Text Available The development of resistant varieties and hybrid combinations has been the most effective and economical strategy to control blast disease caused by Magnaporthe oryzae. However, the distribution of major R genes and blast resistance characterization in hybrid rice parents has not been well investigated, resulting in their limited use in hybrid rice blast-resistance breeding. In the present study, 88 elite indica hybrid rice parental lines were evaluated with 30 isolates of M. oryzae collected from the main planting area of indica hybrid rice in China and were characterized for the presence of 11 major resistance genes using molecular markers. The pathogenicity assays showed that four types of hybrid rice parent line showed some resistance to M. oryzae. However, the proportions of highly resistant lines and the mean resistance frequency (RF varied among the four types, with resistance in decreasing order shown by three-line restorer lines, three-line maintainer lines, two-line sterile lines, and two-line restorer lines. All 88 hybrid rice parental lines carried more than one R gene, but none carried the R genes Pi1 and Pi2. Although Pid3 and Pi9 were present only in three-line restorer lines and Pigm only in three-line maintainer lines, the remaining six R genes (Pib, Pid2, Pi5, Pia, Pi54, and Pita were present in the four types of hybrid rice parent with significantly different distribution frequencies. The correlation between R genes and resistance reactions was investigated. The results are expected to provide useful information for rational utilization of major R genes in hybrid rice breeding programs. Keywords: Hybrid rice parental lines, Magnaporthe oryzae, Pi genes, Resistance evaluation, Molecular markers

  9. In-line digital holographic sensor for monitoring and characterizing marine particulates

    Owen, Robert B.; Zozulya, Alex A.

    2000-01-01

    We report an in-line digital holographic sensor (DHS) for monitoring and characterizing marine particulates. This system images individual particles over a deep depth of field (>25 cm) with a resolution of 5 μm. The DHS projects a collimated beam through the water column and onto a lensless CCD array. Some light is diffracted by particulates and forms an object beam; the undeflected remainder constitutes the reference beam. The two beams combine at the CCD array and create an in-line hologram, which is then numerically reconstructed. The DHS eliminates many problems traditionally associated with holography. The CCD recording material considerably lowers the exposure time and eliminates most vibration problems. The laser power needs are low; the DHS uses a small 10-mW diode laser. Rapid numerical reconstruction eliminates photographic processing and optical reconstruction. We successfully operated the DHS underwater on a remotely operated vehicle; our test results include tracing a single particle from one hologram to the next, thus deriving a velocity vector for marine mass transport. We outline our digital holographic reconstruction procedure, and present our graphical user interface and user software tools. The DHS is particularly useful for providing in situ ground-truth measurements for environmental remote sensing. (c) 2000 Society of Photo-Optical Instrumentation Engineers

  10. Establishment and characterization of CAG/EGFP transgenic rabbit line.

    Takahashi, Ri-ichi; Kuramochi, Takashi; Aoyagi, Kazuki; Hashimoto, Shu; Miyoshi, Ichiro; Kasai, Noriyuki; Hakamata, Yoji; Kobayashi, Eiji; Ueda, Masatsugu

    2007-02-01

    Cell marking is a very important procedure for identifying donor cells after cell and/or organ transplantation in vivo. Transgenic animals expressing marker proteins such as enhanced green fluorescent protein (EGFP) in their tissues are a powerful tool for research in fields of tissue engineering and regenerative medicine. The purpose of this study was to establish transgenic rabbit lines that ubiquitously express EGFP under the control of the cytomegalovirus immediate early enhancer/beta-actin promoter (CAG) to provide a fluorescent transgenic animal as a bioresource. We microinjected the EGFP expression vector into 945 rabbit eggs and 4 independent transgenic candidate pups were obtained. Two of them died before sexual maturation and one was infertile. One transgenic male candidate founder rabbit was obtained and could be bred by artificial insemination. The rabbit transmitted the transgene in a Mendelian manner. Using fluorescence in situ hybridization analysis, we detected the transgene at 7q11 on chromosome 7 as a large centromeric region in two F1 offspring (one female and one male). Eventually, one transgenic line was established. Ubiquitous EGFP fluorescence was confirmed in all examined organs. There were no gender-related differences in fluorescence. The established CAG/EGFP transgenic rabbit will be an important bioresource and a useful tool for various studies in tissue engineering and regenerative medicine.

  11. Final Hanford Site Transuranic (TRU) Waste Characterization QA Project Plan

    GREAGER, T.M.

    2000-01-01

    The Quality Assurance Project Plan (QAPjP) has been prepared for waste characterization activities to be conducted by the Transuranic (TRU) Project at the Hanford Site to meet requirements set forth in the Waste Isolation Pilot Plan (WIPP) Hazardous Waste Facility Permit, 4890139088-TSDF, Attachment B, including Attachments B1 through B6 (WAP) (DOE, 1999a). The QAPjP describes the waste characterization requirements and includes test methods, details of planned waste sampling and analysis, and a description of the waste characterization and verification process. In addition, the QAPjP includes a description of the quality assurance/quality control (QA/QC) requirements for the waste characterization program. Before TRU waste is shipped to the WIPP site by the TRU Project, all applicable requirements of the QAPjP shall be implemented. Additional requirements necessary for transportation to waste disposal at WIPP can be found in the ''Quality Assurance Program Document'' (DOE 1999b) and HNF-2600, ''Hanford Site Transuranic Waste Certification Plan.'' TRU mixed waste contains both TRU radioactive and hazardous components, as defined in the WLPP-WAP. The waste is designated and separately packaged as either contact-handled (CH) or remote-handled (RH), based on the radiological dose rate at the surface of the waste container. RH TRU wastes are not currently shipped to the WIPP facility

  12. Characterization of the camel skin cell line Dubca.

    Klopries, M; Wernery, U; Kaaden, O R

    1995-01-01

    A skin fibroblast cell culture was established from a 2-month-old dromedary foetus. The cells were transformed by infection with SV40 and cloned in soft agar. The established cell line is now designated Dubca cells (Dubai camel) and has been in permanent culture for 95 passages. The cell culture was examined morphologically, chromosome preparations made and DNA fingerprinting performed by hybridization with the oligonucleotide probe (GTG)5. SV40 large T antigen was detected by western blotting. The viral host range was determined by infection with viruses of different families. Camelpox virus (CaPV) bovine herpesvirus-1 (BHV-1), vesicular stomatitis virus (VSV) and border disease virus (BDV) could be propagated in these cells.

  13. Impulsive Noise Characterization in Narrowband Power Line Communication

    Li Bai

    2018-04-01

    Full Text Available Currently, narrowband Power line communication (PLC is considered an attractive communication system in smart grid environments for applications such as advanced metering infrastructure (AMI. In this paper, we will present a comprehensive comparison and analysis in time and frequency domain of noise measured in China and Italy. In addition, impulsive noise in these two countries are mainly analyzed and modeled using two probability based models, Middleton Class A (MCA model and α stable distribution model. The results prove that noise measured in China is rich in impulsive noise, and can be modeled well by α stable distribution model, while noise measured in Italy has less impulsive noise, and can be better modeled by the MCA model.

  14. Quality assurance program plan for SNF characterization support project

    Tanke, J.M.

    1997-01-01

    This Quality Assurance Program Plan (QAPP) provides information on how the Quality Assurance Program is implemented for the Spent Nuclear Fuel Characterization Support Project. This QAPP has been developed specifically for the Spent Nuclear Fuel Characterization Support Project, per Letter of Instruction (LOI) from Duke Engineering and Services Company, letter No. DESH-9655870, dated Nov. 22, 1996. It applies to those items and tasks which affect the completion of activities identified in the work breakdown structure of the Project Management Plan (PMP) and LOI. These activities include installation of sectioning equipment and furnace, surface and subsurface examinations, sectioning for metallography, and element drying and conditioning testing, as well as project related operations within the 327 facility as it relates to the specific activities of this project. General facility activities are covered in other appropriate QA-PPS. In addition, this QAPP supports the related quality assurance activities addressed in CM-2-14, Hazardous Material Packaging and Shipping,1261 and HSRCM-1, Hanford Site Radiological Control Manual. The 327 Building is currently transitioning from being a Pacific Northwest National Laboratory (PNNL) managed facility to a Babcock and Wilcox Hanford Company (BVMC) managed facility. During this transition process existing procedures and documents will be utilized until replaced by BVMC procedures and documents. These documents conform to the requirements found in PNL-MA-70, Quality Assurance Manual and PNL-MA-8 1, Hazardous Materials Shipping Manual. The Quality Assurance Program Index (QAPI) contained in Table 1 provides a matrix which shows how project activities relate to IO CFR 830.120 and 5700.6C criteria. Quality Assurance program requirements will be addressed separate from the requirements specified in this document. Other Hanford Site organizations/companies may be utilized in support of this project and the subject organizations are

  15. Characterization of cell lines stably transfected with rubella virus replicons

    Tzeng, Wen-Pin; Xu, Jie; Frey, Teryl K.

    2012-01-01

    Rubella virus (RUBV) replicons expressing a drug resistance gene and a gene of interest were used to select cell lines uniformly harboring the replicon. Replicons expressing GFP and a virus capsid protein GFP fusion (C-GFP) were compared. Vero or BHK cells transfected with either replicon survived drug selection and grew into a monolayer. However, survival was ∼9-fold greater following transfection with the C-GFP-replicon than with the GFP-expressing replicon and while the C-GFP-replicon cells grew similarly to non-transfected cells, the GFP-replicon cells grew slower. Neither was due to the ability of the CP to enhance RNA synthesis but survival during drug selection was correlated with the ability of CP to inhibit apoptosis. Additionally, C-GFP-replicon cells were not cured of the replicon in the absence of drug selection. Interferon-alpha suppressed replicon RNA and protein synthesis, but did not cure the cells, explaining in part the ability of RUBV to establish persistent infections.

  16. Characterization of cell lines stably transfected with rubella virus replicons

    Tzeng, Wen-Pin; Xu, Jie [Department of Biology, Georgia State University, P.O. Box 4010, Atlanta GA 30302-4010 (United States); Frey, Teryl K., E-mail: tfrey@gsu.edu [Department of Biology, Georgia State University, P.O. Box 4010, Atlanta GA 30302-4010 (United States)

    2012-07-20

    Rubella virus (RUBV) replicons expressing a drug resistance gene and a gene of interest were used to select cell lines uniformly harboring the replicon. Replicons expressing GFP and a virus capsid protein GFP fusion (C-GFP) were compared. Vero or BHK cells transfected with either replicon survived drug selection and grew into a monolayer. However, survival was {approx}9-fold greater following transfection with the C-GFP-replicon than with the GFP-expressing replicon and while the C-GFP-replicon cells grew similarly to non-transfected cells, the GFP-replicon cells grew slower. Neither was due to the ability of the CP to enhance RNA synthesis but survival during drug selection was correlated with the ability of CP to inhibit apoptosis. Additionally, C-GFP-replicon cells were not cured of the replicon in the absence of drug selection. Interferon-alpha suppressed replicon RNA and protein synthesis, but did not cure the cells, explaining in part the ability of RUBV to establish persistent infections.

  17. Nahm transformation for parabolic Higgs bundles on the projective line --- case of non-semisimple residues

    Szabo, Szilard

    2016-01-01

    We extend our earlier construction of Nahm transformation for parabolic Higgs bundles on the projective line to solutions with not necessarily semisimple residues and show that it determines a holomorphic mapping on corresponding moduli spaces. The construction relies on suitable elementary modifications of the logarithmic Dolbeault complex.

  18. Flatiron-Erie 115kV transmission line project, Larimer, Weld and Boulder Counties, Colorado

    1993-05-01

    Western Area Power Administration (Western) proposes to uprate its existing 115-kV Flatiron-Erie transmission line. The line is located in Larimer, Weld and Boulder Counties, Colorado, and passes through the City of Longmont. The line connects Flatiron Substation and several of the substations supplying Longmont. It is a single circuit 115-kV line, 31.5 miles long, and was built in 1950-51 on a 75-foot wide right-of-way (ROW) using wood H-frame structures. Western proposes to build 27 new structures along the line, to replace or modify 45 of the existing structures and to remote 11 of them. Many of these additions and changes would involve structures that are approximately 5 to 15 feet taller than the existing ones. The existing conductors and ground wires would remain in place. The purpose of these actions would be to allow the power carrying capability of the line to be increased and to replace deteriorating/structural members. Western would be the sole participant in the proposed project. This report gives an analysis of the study area environment and the development of alternative routes. An assessment is presented of the impacts of the primary alternative routes. The environmental consequences of this project are addressed

  19. Identification and characterization of mobile genetic elements LINEs from Brassica genome.

    Nouroz, Faisal; Noreen, Shumaila; Khan, Muhammad Fiaz; Ahmed, Shehzad; Heslop-Harrison, J S Pat

    2017-09-05

    Among transposable elements (TEs), the LTR retrotransposons are abundant followed by non-LTR retrotransposons in plant genomes, the lateral being represented by LINEs and SINEs. Computational and molecular approaches were used for the characterization of Brassica LINEs, their diversity and phylogenetic relationships. Four autonomous and four non-autonomous LINE families were identified and characterized from Brassica. Most of the autonomous LINEs displayed two open reading frames, ORF1 and ORF2, where ORF1 is a gag protein domain, while ORF2 encodes endonuclease (EN) and a reverse transcriptase (RT). Three of four families encoded an additional RNase H (RH) domain in pol gene common to 'R' and 'I' type of LINEs. The PCR analyses based on LINEs RT fragments indicate their high diversity and widespread occurrence in tested 40 Brassica cultivars. Database searches revealed the homology in LINE sequences in closely related genera Arabidopsis indicating their origin from common ancestors predating their separation. The alignment of 58 LINEs RT sequences from Brassica, Arabidopsis and other plants depicted 4 conserved domains (domain II-V) showing similarity to previously detected domains. Based on RT alignment of Brassica and 3 known LINEs from monocots, Brassicaceae LINEs clustered in separate clade, further resolving 4 Brassica-Arabidopsis specific families in 2 sub-clades. High similarities were observed in RT sequences in the members of same family, while low homology was detected in members across the families. The investigation led to the characterization of Brassica specific LINE families and their diversity across Brassica species and their cultivars. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Reasons for decision in the matter of Enbridge Pipelines Inc. Line 4 Extension Project : facilities

    2008-01-01

    In June 2007, Enbridge Pipelines Inc. applied for approval to extend Line 4 of its Mainline pipeline upstream from Hardisty to Edmonton, Alberta. The project would relieve a potential bottleneck and would also add an additional line across this segment to increase system security and flexibility. The average annual capacity of Line 4 would be 140,000 cubic metres per day. The Enbridge Mainline from Edmonton to Hardisty currently includes 3 oil pipelines, while the Enbridge Mainline downstream of Hardisty includes 4 oil pipelines. This document presented the views of the Board regarding the construction and operation of the project facilities; public and Aboriginal consultation; environmental and socio-economic matters; land matters; tolls and tariffs; and, supply, markets, financing and economics. The Board noted that the benefits of this project outweigh the burdens associated with it. The project required an environmental assessment under the Canadian Environmental Assessment Act which found that it is not likely to cause significant adverse environmental effects. In its approval of the project, the Board attached 15 conditions, including a requirement for Enbridge to file an updated Environmental Protection Plan for the Board's approval. The Board also approved Enbridge's application for its tolling method and to reactivate 3 sections of pre-existing pipeline. 3 tabs., 3 figs., 3 appendices

  1. Genetic modification of the human germ line: The reasons why this project has no future.

    Morange, Michel

    2015-01-01

    Modification of the human germ line has remained a distant but valuable objective for most biologists since the emergence of genetics (and even before). To study the historical transformations of this project, I have selected three periods - the 1930s, at the pinnacle of eugenics, around 1974 when molecular biology triumphed, and today - and have adopted three criteria to estimate the feasibility of this project: the state of scientific knowledge, the existence of suitable tools, and societal demands. Although the long-awaited techniques to modify the germ line are now available, I will show that most of the expectations behind this project have disappeared, or are considered as being reachable by highly different strategies. Copyright © 2015 Académie des sciences. Published by Elsevier SAS. All rights reserved.

  2. Global Threat Reduction Initiative Fuel Thermo-Physical Characterization Project: Sample Management Plan

    Casella, Amanda J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Pereira, Mario M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Steen, Franciska H. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2013-01-01

    This sample management plan provides guidelines for sectioning, preparation, acceptance criteria, analytical path, and end-of-life disposal for the fuel element segments utilized in the Global Threat Reduction Initiative (GTRI), Fuel Thermo-Physical Characterization Project. The Fuel Thermo-Physical Characterization Project is tasked with analysis of irradiated Low Enriched Uranium (LEU) Molybdenum (U-Mo) fuel element samples to support the GTRI conversion program. Sample analysis may include optical microscopy (OM), scanning electron microscopy (SEM) fuel-surface interface analysis, gas pycnometry (density) measurements, laser flash analysis (LFA), differential scanning calorimetry (DSC), thermogravimetry and differential thermal analysis with mass spectroscopy (TG /DTA-MS), Inductively Coupled Plasma Spectrophotometry (ICP), alpha spectroscopy, and Thermal Ionization Mass Spectroscopy (TIMS). The project will utilize existing Radiochemical Processing Laboratory (RPL) operating, technical, and administrative procedures for sample receipt, processing, and analyses. Test instructions (TIs), which are documents used to provide specific details regarding the implementation of an existing RPL approved technical or operational procedure, will also be used to communicate to staff project specific parameters requested by the Principal Investigator (PI). TIs will be developed, reviewed, and issued in accordance with the latest revision of the RPL-PLN-700, RPL Operations Plan. Additionally, the PI must approve all project test instructions and red-line changes to test instructions.

  3. TWRS privatization support project waste characterization database development

    1995-11-01

    Pacific Northwest National Laboratory requested support from ICF Kaiser Hanford Company in assembling radionuclide and chemical analyte sample data and inventory estimates for fourteen Hanford underground storage tanks: 241-AN-102, -104, -105, -106, and -107, 241-AP-102, -104, and -105, 241-AW-101, -103, and -105, 241 AZ-101 and -102; and 241-C-109. Sample data were assembled for sixteen radionuclides and thirty-five chemical analytes. The characterization data were provided to Pacific Northwest National Laboratory in support of the Tank Waste Remediation Services Privatization Support Project. The purpose of this report is to present the results and document the methodology used in preparing the waste characterization information data set to support the Tank Waste Remediation Services Privatization Support Project. This report describes the methodology used in assembling the waste characterization information and how that information was validated by a panel of independent technical reviewers. Also, contained in this report are the various data sets created: the master data set, a subset, and an unreviewed data set. The master data set contains waste composition information for Tanks 241-AN-102 and -107, 241-AP-102 and -105, 241-AW-101; and 241-AZ-101 and -102. The subset contains only the validated analytical sample data from the master data set. The unreviewed data set contains all collected but unreviewed sample data for Tanks 241-AN-104, -105, and -106; 241-AP-104; 241-AW-103 and-105; and 241-C-109. The methodology used to review the waste characterization information was found to be an accurate, useful way to separate the invalid or questionable data from the more reliable data. In the future, this methodology should be considered when validating waste characterization information

  4. Visualization and characterization of users in a citizen science project

    Morais, Alessandra M. M.; Raddick, Jordan; Coelho dos Santos, Rafael D.

    2013-05-01

    Recent technological advances allowed the creation and use of internet-based systems where many users can collaborate gathering and sharing information for specific or general purposes: social networks, e-commerce review systems, collaborative knowledge systems, etc. Since most of the data collected in these systems is user-generated, understanding of the motivations and general behavior of users is a very important issue. Of particular interest are citizen science projects, where users without scientific training are asked for collaboration labeling and classifying information (either automatically by giving away idle computer time or manually by actually seeing data and providing information about it). Understanding behavior of users of those types of data collection systems may help increase the involvement of the users, categorize users accordingly to different parameters, facilitate their collaboration with the systems, design better user interfaces, and allow better planning and deployment of similar projects and systems. Behavior of those users could be estimated through analysis of their collaboration track: registers of which user did what and when can be easily and unobtrusively collected in several different ways, the simplest being a log of activities. In this paper we present some results on the visualization and characterization of almost 150.000 users with more than 80.000.000 collaborations with a citizen science project - Galaxy Zoo I, which asked users to classify galaxies' images. Basic visualization techniques are not applicable due to the number of users, so techniques to characterize users' behavior based on feature extraction and clustering are used.

  5. TWRS privatization support project waste characterization database development. Volume 1

    Brevick, C.H.

    1995-11-01

    Pacific Northwest National Laboratory requested support from ICF Kaiser Hanford Company in assembling radionuclide and chemical analyte sample data and inventory estimates for fourteen Hanford under-ground storage tanks: 241-AN-102, -104, -105, -106, and -107, 241-AP-102, -104, and -105; 241-AW-101, -103, and -105, 241-AZ-101 and-102; and 241-C-109. Sample data were assembled for sixteen radio nuclides and thirty five chemical analytes. The characterization data were provided to Pacific Northwest National Laboratory in support of the Tank Waste Remediation Services Privatization Support Project. The purpose of this report is to present the results and document the methodology used in preparing the waste characterization information data set to support the Tank Waste Remediation Services Privatization Support Project. This report describes the methodology used in assembling the waste characterization information and how that information was validated by a panel of independent technical reviewers. Also, contained in this report are the various data sets created., the master data set, a subset, and an unreviewed data set

  6. Manitoba Hydro long-term high-voltage transmission line magnetic field monitoring project

    Wong, P.S.; Ng, C.K.

    2008-01-01

    As part of the licensing process to construct a new 230 kV transmission line on an existing right-of-way in Manitoba, an electrical effects study was conducted in 1998. The study was part of the environmental assessment program crucial in obtaining government approval to construct the line. Some residents living adjacent to the new transmission circuit expressed concerns about alleged adverse health effects associated with long-term exposure to magnetic fields from high voltage transmission lines. In order to verify the accuracy of the predicted magnetic field levels submitted to the regulatory body in the the electrical effects study and to instill confidence in the residents of the affected communities, a three-year magnetic monitoring project was conducted between 2003 and 2005 along the right-of-way after the new 230kV transmission line was energized by Manitoba Hydro. This paper described the monitoring program, with reference to location; equipment; data analysis; and discussion of results. It was concluded that the long-term monitoring project demonstrated that the magnetic field prediction methodology was well understood and accurate, and provided valuable long-term magnetic field characteristics at the edge of the right-of-way. In addition, when there is opposition to a transmission line, public consultation and education were found to be the best options to arrive at a solution. 3 refs., 1 tab., 12 figs

  7. Marys Lake 69/115-kV transmission line upgrade and substation expansion projects

    NONE

    1996-05-01

    Western Area Power Administration (Western) and the Platte River Power Authority (Platte River) propose to upgrade portions of the existing electric transmission and substation system that serves the Town of Estes Park, Colorado. The existing transmission lines between the Estes Power Plant Switchyard and the Marys Lake Substation include a 115,000 volt (115-kV) line and 69,000 volt (69-kV) line. Approximately one mile is a double-circuit 115/69-kV line on steel lattice structures, and approximately two miles consists of separate single-circuit 115-kV and a 69-kV lines, constructed on wood H-Frame structures. Both lines were constructed in 1951 by the US Bureau of Reclamation. The existing transmission lines are on rights-of-way (ROW) that vary from 75 feet to 120 feet and are owned by Western. There are 48 landowners adjacent to the existing ROW. All of the houses were built adjacent to the existing ROW after the transmission lines were constructed. Upgrading the existing 69-kV transmission line between the Marys Lake Substation and the Estes Power Plant Switchyard to 115-kV and expanding the Marys Lake Substation was identified as the most effective way in which to improve electric service to Estes Park. The primary purpose and need of the proposed project is to improve the reliability of electric service to the Town of Estes Park. Lack of reliability has been a historical concern, and reliability will always be less than desired until physical improvements are made to the electrical facilities serving Estes Park.

  8. Marys Lake 69/115-kV transmission line upgrade and substation expansion projects

    1996-05-01

    Western Area Power Administration (Western) and the Platte River Power Authority (Platte River) propose to upgrade portions of the existing electric transmission and substation system that serves the Town of Estes Park, Colorado. The existing transmission lines between the Estes Power Plant Switchyard and the Marys Lake Substation include a 115,000 volt (115-kV) line and 69,000 volt (69-kV) line. Approximately one mile is a double-circuit 115/69-kV line on steel lattice structures, and approximately two miles consists of separate single-circuit 115-kV and a 69-kV lines, constructed on wood H-Frame structures. Both lines were constructed in 1951 by the US Bureau of Reclamation. The existing transmission lines are on rights-of-way (ROW) that vary from 75 feet to 120 feet and are owned by Western. There are 48 landowners adjacent to the existing ROW. All of the houses were built adjacent to the existing ROW after the transmission lines were constructed. Upgrading the existing 69-kV transmission line between the Marys Lake Substation and the Estes Power Plant Switchyard to 115-kV and expanding the Marys Lake Substation was identified as the most effective way in which to improve electric service to Estes Park. The primary purpose and need of the proposed project is to improve the reliability of electric service to the Town of Estes Park. Lack of reliability has been a historical concern, and reliability will always be less than desired until physical improvements are made to the electrical facilities serving Estes Park

  9. Establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts.

    Hong Xin

    Full Text Available Hepatocellular carcinoma (HCC is a common cancer with poor prognosis worldwide and the molecular mechanism is not well understood. This study aimed to establish a collection of human HCC cell lines from patient-derived xenograft (PDX models. From the 20 surgical HCC sample collections, 7 tumors were successfully developed in immunodeficient mice and further established 7 novel HCC cell lines (LIXC002, LIXC003, LIXC004, LIXC006, LIXC011, LIXC012 and CPL0903 by primary culture. The characterization of cell lines was defined by morphology, growth kinetics, cell cycle, chromosome analysis, short tandem repeat (STR analysis, molecular profile, and tumorigenicity. Additionally, response to clinical chemotherapeutics was validated both in vitro and in vivo. STR analysis indicated that all cell lines were unique cells different from known cell lines and free of contamination by bacteria or mycoplasma. The other findings were quite heterogeneous between individual lines. Chromosome aberration could be found in all cell lines. Alpha-fetoprotein was overexpressed only in 3 out of 7 cell lines. 4 cell lines expressed high level of vimentin. Ki67 was strongly stained in all cell lines. mRNA level of retinoic acid induced protein 3 (RAI3 was decreased in all cell lines. The 7 novel cell lines showed variable sensitivity to 8 tested compounds. LIXC011 and CPL0903 possessed multiple drug resistance property. Sorafenib inhibited xenograft tumor growth of LIXC006, but not of LIXC012. Our results indicated that the 7 novel cell lines with low passage maintaining their clinical and pathological characters could be good tools for further exploring the molecular mechanism of HCC and anti-cancer drug screening.

  10. Uranium soils integrated demonstration: Soil characterization project report

    Cunnane, J.C.; Gill, V.R.; Lee, S.Y.; Morris, D.E.; Nickelson, M.D.; Perry, D.L.; Tidwell, V.C.

    1993-08-01

    An Integrated Demonstration Program, hosted by the Fernald Environmental Management Project (FEMP), has been established for investigating technologies applicable to the characterization and remediation of soils contaminated with uranium. Critical to the design of relevant treatment technologies is detailed information on the chemical and physical characteristics of the uranium waste-form. To address this need a soil sampling and characterization program was initiated which makes use of a variety of standard analytical techniques coupled with state-of-the-art microscopy and spectroscopy techniques. Sample representativeness is evaluated through the development of conceptual models in an effort to identify and understand those geochemical processes governing the behavior of uranium in FEMP soils. Many of the initial results have significant implications for the design of soil treatment technologies for application at the FEMP

  11. Uranium soils integrated demonstration: Soil characterization project report

    Cunnane, J.C. [Argonne National Lab., IL (United States); Gill, V.R. [Fernald Environmental Restoration Management Corp., Cincinnati, OH (United States); Lee, S.Y. [Oak Ridge National Lab., TN (United States); Morris, D.E. [Los Alamos National Lab., NM (United States); Nickelson, M.D. [HAZWRAP, Oak Ridge, TN (United States); Perry, D.L. [Lawrence Berkeley Lab., CA (United States); Tidwell, V.C. [Sandia National Labs., Albuquerque, NM (United States)

    1993-08-01

    An Integrated Demonstration Program, hosted by the Fernald Environmental Management Project (FEMP), has been established for investigating technologies applicable to the characterization and remediation of soils contaminated with uranium. Critical to the design of relevant treatment technologies is detailed information on the chemical and physical characteristics of the uranium waste-form. To address this need a soil sampling and characterization program was initiated which makes use of a variety of standard analytical techniques coupled with state-of-the-art microscopy and spectroscopy techniques. Sample representativeness is evaluated through the development of conceptual models in an effort to identify and understand those geochemical processes governing the behavior of uranium in FEMP soils. Many of the initial results have significant implications for the design of soil treatment technologies for application at the FEMP.

  12. Characterization and drug resistance patterns of Ewing's sarcoma family tumor cell lines.

    William A May

    Full Text Available Despite intensive treatment with chemotherapy, radiotherapy and surgery, over 70% of patients with metastatic Ewing's Sarcoma Family of Tumors (EFT will die of their disease. We hypothesize that properly characterized laboratory models reflecting the drug resistance of clinical tumors will facilitate the application of new therapeutic agents to EFT. To determine resistance patterns, we studied newly established EFT cell lines derived from different points in therapy: two established at diagnosis (CHLA-9, CHLA-32, two after chemotherapy and progressive disease (CHLA-10, CHLA-25, and two at relapse after myeloablative therapy and autologous bone marrow transplantation (post-ABMT (CHLA-258, COG-E-352. The new lines were compared to widely studied EFT lines TC-71, TC-32, SK-N-MC, and A-673. These lines were extensively characterized with regard to identity (short tandem repeat (STR analysis, p53, p16/14 status, and EWS/ETS breakpoint and target gene expression profile. The DIMSCAN cytotoxicity assay was used to assess in vitro drug sensitivity to standard chemotherapy agents. No association was found between drug resistance and the expression of EWS/ETS regulated genes in the EFT cell lines. No consistent association was observed between drug sensitivity and p53 functionality or between drug sensitivity and p16/14 functionality across the cell lines. Exposure to chemotherapy prior to cell line initiation correlated with drug resistance of EFT cell lines in 5/8 tested agents at clinically achievable concentrations (CAC or the lower tested concentration (LTC: (cyclophosphamide (as 4-HC and doxorubicin at CAC, etoposide, irinotecan (as SN-38 and melphalan at LTC; P<0.1 for one agent, and P<0.05 for four agents. This panel of well-characterized drug-sensitive and drug-resistant cell lines will facilitate in vitro preclinical testing of new agents for EFT.

  13. The Rocky Flats Environmental Technology Site beryllium characterization project

    Morrell, D.M.; Miller, J.R.; Allen, D.F.

    1999-01-01

    A site beryllium characterization project was completed at the Rocky Flats Environmental Technology Site (RFETS) in 1997. Information from historical reviews, previous sampling surveys, and a new sampling survey were used to establish a more comprehensive understanding of the locations and levels of beryllium contamination in 35 buildings. A feature of the sampling strategy was to test if process knowledge was a good predictor of where beryllium contamination could be found. Results revealed that this technique was effective at identifying where surface contamination levels might exceed the RFETS smear control level but that it was not effective in identifying where low concentrations of beryllium might be found

  14. Characterizing symmetries in a projected entangled pair state

    Perez-Garcia, D; Gonzalez-Guillen, C E [Departamento Analisis Matematico and IMI, Universidad Complutense de Madrid, 28040 Madrid (Spain); Sanz, M; Cirac, J I [Max-Planck-Institut fuer Quantenoptik, Hans-Kopfermann-Str. 1, 85748 Garching (Germany); Wolf, M M [Niels Bohr Institute, Blegdamsvej 17, 2100 Copenhagen (Denmark)], E-mail: dperez@mat.ucm.es

    2010-02-15

    We show that two different tensors defining the same translational invariant injective projected entangled pair state (PEPS) in a square lattice must be the same up to a trivial gauge freedom. This allows us to characterize the existence of any local or spatial symmetry in the state. As an application of these results we prove that a SU(2) invariant PEPS with half-integer spin cannot be injective, which can be seen as a Lieb-Shultz-Mattis theorem in this context. We also give the natural generalization for U(1) symmetry in the spirit of Oshikawa-Yamanaka-Affleck, and show that a PEPS with Wilson loops cannot be injective.

  15. Development and characterization of a cell line WAF from freshwater shark Wallago attu.

    Dubey, Akhilesh; Goswami, Mukunda; Yadav, Kamalendra; Sharma, Bhagwati S

    2014-02-01

    A new epithelial cell line, WAF was developed from caudal fin of freshwater shark, Wallago attu. The cell line was optimally maintained at 28 °C in Leibovitz-15 (L-15) medium supplemented with 20 % fetal bovine serum. The cell line was characterized by various cytogenetic and molecular markers. The cytogenetic analysis revealed a diploid count of 86 chromosomes at different passages. The origin of the cell lines was confirmed by the amplification of 547 and 654 bp sequences of 16S rRNA and cytochrome oxidase subunit I genes of mitochondrial DNA, respectively. WAF cells were characterized for their growth characteristics at different temperature and serum concentration. Epithelial morphology of the cell line was confirmed using immunocytochemistry. Further cell plating efficiency, transfection efficiency and viability of cryopreserved WAF cells was also determined. Cytotoxicity and genotoxicity assessment of cadmium salts on WAF cells by MTT, NR and comet assay illustrated the utility of this cell line as an in vitro model for aquatic toxicological studies. The cell line will be further useful for studying oxidative stress markers against aquatic pollutants.

  16. Derivation and Osmotolerance Characterization of Three Immortalized Tilapia (Oreochromis mossambicus) Cell Lines

    Gardell, Alison M.; Qin, Qin; Rice, Robert H.; Li, Johnathan; Kültz, Dietmar

    2014-01-01

    Fish cell cultures are becoming more widely used models for investigating molecular mechanisms of physiological response to environmental challenge. In this study, we derived two immortalized Mozambique tilapia (Oreochromis mossambicus) cell lines from brain (OmB) and lip epithelium (OmL), and compared them to a previously immortalized bulbus arteriosus (TmB) cell line. The OmB and OmL cell lines were generated without or with Rho-associated kinase (ROCK) inhibitor/3T3 feeder layer supplementation. Although both approaches were successful, ROCK inhibitor/feeder layer supplementation was found to offer the advantages of selecting for epithelial-like cell type and decreasing time to immortalization. After immortalization (≥ passage 5), we characterized the proteomes of the newly derived cell lines (OmB and OmL) using LCMS and identified several unique cell markers for each line. Subsequently, osmotolerance for each of the three cell lines following acute exposure to elevated sodium chloride was evaluated. The acute maximum osmotolerance of these tilapia cell lines (>700 mOsm/kg) was markedly higher than that of any other known vertebrate cell line, but was significantly higher in the epithelial-like OmL cell line. To validate the physiological relevance of these tilapia cell lines, we quantified the effects of acute hyperosmotic challenge (450 mOsm/kg and 700 mOsm/kg) on the transcriptional regulation of two enzymes involved in biosynthesis of the compatible organic osmolyte, myo-inositol. Both enzymes were found to be robustly upregulated in all three tilapia cell lines. Therefore, the newly established tilapia cells lines represent valuable tools for studying molecular mechanisms involved in the osmotic stress response of euryhaline fish. PMID:24797371

  17. The Help-line "Invito alla Vita": a new project for suicide prevention in Trentino region.

    Di Napoli, Wilma; Andreatta, Olaf

    2014-11-01

    "Invito alla Vita" is a community-based suicide prevention project that officially started in Trentino in late 2008. The project was promoted by the local Health Services, trying from the beginning to involve other community subjects, and has been working over five years and a half in different directions, particularly promoting a phone help-line. The aims of the Invito alla Vita (IaV) Help Line have been clear from the beginning: decrease the sense of loneliness, offer encouragement and support, promote engagement with health services, reduce stigma and prejudice. Contrary to popular misconceptions, talking with people about suicide will not increase suicide risk, neither will it induce patients to commit suicide.The volunteers involved in the IaV help-line offer people empathic listening without judgment and easy tips, to reduce loneliness, sadness and supply reassurance that other people care. In this study we tried to deepen our knowledges about the volunteers' motivations and necessities to use them for creating a better system of support: we realized indeed that continuous training and supervision, along with official awards given by community institutions, are basic factors to sustain the volunteers' motives to cooperate with the help line.

  18. Establishment and conventional cytogenetic characterization of three gastric cancer cell lines.

    Leal, Mariana Ferreira; Martins do Nascimento, José Luiz; da Silva, Carla Elvira Araújo; Vita Lamarão, Maria Fernanda; Calcagno, Danielle Queiroz; Khayat, André Salim; Assumpção, Paulo Pimentel; Cabral, Isabel Rosa; de Arruda Cardoso Smith, Marília; Burbano, Rommel Rodríguez

    2009-11-01

    Gastric cancer is the fourth most frequent type of cancer and the second most frequent cause of cancer mortality worldwide. Only a modest number of gastric carcinoma cell lines have been isolated thus far. Here we describe the establishment and cytogenetic characterization of three new gastric cancer cell lines obtained from primary gastric adenocarcinoma (ACP02 and ACP03) and cancerous ascitic fluid (AGP01) of individuals from northern Brazil. ACP02, ACP03, and AGP01 cell lines are presently in the 60th passage. The cell lines grew in a disorganized single layer with some agglomerations and heterogeneous divisions (bipolar and multipolar). All cell lines exhibited a composite karyotype with several clonal chromosome alterations. Trisomy 8 was the most frequent alteration. Chromosome 8 aneusomy was confirmed by fluorescence in situ hybridization. All cell lines also exhibited trisomy 7 and deletion of chromosome arm 17p. These results suggest that, although frequent chromosome alterations are commonly observed due to culture process, the ACP02, ACP03, and AGP01 cell lines and primary gastric cancer from individuals of northern Brazil share genetic alterations, supporting use of these cell lines as a model of gastric carcinogenesis in this population.

  19. Technical data management at the Yucca Mountain Site Characterization Project

    Statler, J.; Newbury, C.M.; Heitland, G.W.

    1992-01-01

    The Department of Energy/Office of Civilian Radioactive waste Management (DOE/OCRWM) is responsible for the characterization of Yucca Mountain, Nevada, to determine its potential as a site of a high-level radioactive waste repository. The characterization of Yucca Mountain encompasses many diverse investigations, both onsite and in laboratories across the country. Investigations are being conducted of the geology, hydrology, mineralogy, paleoclimate, geotechnical properties, and archeology of the area, to name a few. Effective program management requires that data from site investigations be processed, interpreted and disseminated in a timely manner to support model development and validation, repository design, and performance assessment. The Program must also meet regulatory requirements for making the technical data accessible to a variety of external users throughout the life of the Project. Finally, the DOE/OCRWM must make available the data or its description and access location available for use in support of the license application and supporting documentation. To accomplish these objectives, scientific and engineering data, generated by site characterization activities, and technical data, generated by environmental and socioeconomic impact assessment activities, must be systematically identified, cataloged, stored and disseminated in a controlled manner

  20. Validation of in-line surface characterization by light scattering in Robot Assisted Polishing

    Pilny, Lukas; Bissacco, Giuliano; De Chiffre, Leonardo

    2014-01-01

    The suitability of a commercial scattered light sensor for in-line characterization of fine surfaces in the roughness range Sa 1 – 30 nm generated by the Robot Assisted Polishing (RAP) was investigated and validated. A number of surfaces were generated and directly measured with the scattered light...

  1. Molecular characterization of breast cancer cell lines through multiple omic approaches.

    Smith, Shari E; Mellor, Paul; Ward, Alison K; Kendall, Stephanie; McDonald, Megan; Vizeacoumar, Frederick S; Vizeacoumar, Franco J; Napper, Scott; Anderson, Deborah H

    2017-06-05

    Breast cancer cell lines are frequently used as model systems to study the cellular properties and biology of breast cancer. Our objective was to characterize a large, commonly employed panel of breast cancer cell lines obtained from the American Type Culture Collection (ATCC 30-4500 K) to enable researchers to make more informed decisions in selecting cell lines for specific studies. Information about these cell lines was obtained from a wide variety of sources. In addition, new information about cellular pathways that are activated within each cell line was generated. We determined key protein expression data using immunoblot analyses. In addition, two analyses on serum-starved cells were carried out to identify cellular proteins and pathways that are activated in these cells. These analyses were performed using a commercial PathScan array and a novel and more extensive phosphopeptide-based kinome analysis that queries 1290 phosphorylation events in major signaling pathways. Data about this panel of breast cancer cell lines was also accessed from several online sources, compiled and summarized for the following areas: molecular classification, mRNA expression, mutational status of key proteins and other possible cancer-associated mutations, and the tumorigenic and metastatic capacity in mouse xenograft models of breast cancer. The cell lines that were characterized included 10 estrogen receptor (ER)-positive, 12 human epidermal growth factor receptor 2 (HER2)-amplified and 18 triple negative breast cancer cell lines, in addition to 4 non-tumorigenic breast cell lines. Within each subtype, there was significant genetic heterogeneity that could impact both the selection of model cell lines and the interpretation of the results obtained. To capture the net activation of key signaling pathways as a result of these mutational combinations, profiled pathway activation status was examined. This provided further clarity for which cell lines were particularly deregulated

  2. Cost/CYP: a bottom line that helps keep CSM projects cost-efficient.

    1985-01-01

    In contraceptive social marketing (CSM), the objective is social good, but project managers also need to run a tight ship, trimming costs, allocating scarce funds, and monitoring their program's progress. 1 way CSM managers remain cost-conscious is through the concept of couple-years-of-protection (CYP). Devised 2 decades ago as an administrative tool to compare the effects of different contraceptive methods, CYP's uses have multiplied to include assessing program output and cost effectiveness. Some of the factors affecting cost/CYP are a project's age, sales volume, management efficiency, and product prices and line. These factors are interconnected. The cost/CYP figures given here do not include outlays for commodities. While the Agency for International Development's commodity costs alter slightly with each new purchase contrast, the agency reports that a condom costs about 4 cents (US), an oral contraceptive (OC) cycle about 12 cents, and a spermicidal tablet about 7 cents. CSM projects have relatively high start-up costs. Within a project's first 2 years, expenses must cover such marketing activities as research, packaging, warehousing, and heavy promotion. As a project ages, sales should grow, producing revenues that gradually amortize these initial costs. The Nepal CSM project provides an example of how cost/CYP can improve as a program ages. In 1978, the year sales began, the project's cost/CYP was about $84. For some time the project struggled to get its products to its target market and gradually overcome several major hurdles. The acquisition of jeeps eased distribution and, by adding another condom brand, sales were increased still more, bringing the cost/CYP down to $8.30 in 1981. With further sales increases and resulting revenues, the cost/CYP dropped to just over $7 in 1983. When the sales volume becomes large enough, CSM projects can achieve economies of scale, which greatly improves cost-efficiency. Fixed costs shrink as a proportion of total

  3. Establishment and characterization of a new epithelial cell line, KC-1, from koala (Phascolarctos cinereus) conjunctiva.

    Girjes, Adeeb A; Lee, Kristen E; Carrick, Frank N

    2003-01-01

    A novel, untransformed koala cell line (KC-1) was established by culturing koala conjunctival tissue in growth medium, which has permitted the study of the cell biology of this unique system. After the establishment of the KC-1 cell line, the cells were characterized by light microscopy, doubling time, and Western blot analysis. Light microscopy revealed that the cells have an epithelial morphology. Doubling times were significantly different (P koala cell line was adapted to grow continuously in Dulbecco modified Eagle medium containing 10% FCS for at least 30 passages. This unique cell line is an ideal tool for further investigation on koala cell biology and cytogenetics and for exploration of the pathophysiological mechanism of eye infections caused by different pathogens in koalas.

  4. 75 FR 32940 - Request for Proposals for New or Upgraded Transmission Line Projects Under Section 1222 of the...

    2010-06-10

    ... financial condition and has the ability to secure the necessary financing to meet the Project's requirements... for Proposals for New or Upgraded Transmission Line Projects Under Section 1222 of the Energy Policy..., Department of Energy. ACTION: Request for Project Proposals. SUMMARY: The Department of Energy (DOE or...

  5. Radioactive liquid wastes lines removal project at Los Alamos (1981-1986)

    Elder, J.C.; Cox, E.J.; Hohner, D.P.; Valentine, A.M.

    1986-09-01

    This report describes the abandoned liquid waste lines removal operations conducted at Los Alamos in the period 1981 to 1986. Particular emphasis has been placed on as-left conditions, that is, on the location of sections of waste lines or contaminated soil which were left in place on the basis of ALARA decisions. Contaminated items were left when interfering utilities, roads, structures, or great depth made complete removal not cost effective or not safe. Left items were either not highly contaminated or they were not near the surface. Total cost of the project was $4.2 million. Approximately 5800 m 3 of contaminated waste was placed in the Solid Waste Management Site at TA-54 Area G. The project accomplished the removal of approximately 34,500 ft (6.5 miles) of abandoned waste lines under carefully controlled conditions. Procedures for excavation, waste disposal, personnel protection, and radiation monitoring are described. Environmental monitoring criteria and methods for determining acceptable levels of contamination in soils and on surfaces are discussed

  6. Yucca Mountain Site Characterization Project Waste Package Plan

    Harrison-Giesler, D.J.; Jardine, L.J.

    1991-02-01

    The goal of the US Department of Energy's (DOE) Yucca Mountain Site Characterization Project (YMP) waste package program is to develop, confirm the effectiveness of, and document a design for a waste package and associated engineered barrier system (EBS) for spent nuclear fuel and solidified high-level nuclear waste (HLW) that meets the applicable regulatory requirements for a geologic repository. The Waste Package Plan describes the waste package program and establishes the technical approach against which overall progress can be measured. It provides guidance for execution and describes the essential elements of the program, including the objectives, technical plan, and management approach. The plan covers the time period up to the submission of a repository license application to the US Nuclear Regulatory Commission (NRC). 1 fig

  7. Fluid characterization for miscible EOR projects and CO2 sequestration

    Jessen, Kristian; Stenby, Erling Halfdan

    2007-01-01

    Accurate performance prediction of miscible enhanced-oil-recovery (EOR) projects or CO, sequestration in depleted oil and gas reservoirs relies in part on the ability of an equation-of-state (EOS) model to adequately represent the properties of a wide range of mixtures of the resident fluid...... in the data reduction and demonstrate that for some gas/oil systems, swelling tests do not contribute to a more accurate prediction of multicontact miscibility. Finally, we report on the impact that use of EOS models based on different characterization procedures can have on recovery predictions from dynamic...... and the injected fluid(s). The mixtures that form when gas displaces oil in a porous medium will, in many cases, differ significantly from compositions created in swelling tests and other standard pressure/volume/temperature (PVT) experiments. Multicontact experiments (e.g., slimtube displacements) are often used...

  8. Project plan for the Background Soil Characterization Project on the Oak Ridge Reservation, Oak Ridge, Tennessee

    1992-08-01

    The Background Soil characterization Project (BSCP) will provide background concentration levels of selected metals, organic compounds, and radionuclides in soils from uncontaminated on-site areas at the Oak Ridge Reservation (ORR), and off-site in the western part of Roane County and the eastern part of Anderson County. The BSCP will establish a database, recommend how to use the data for contaminated site assessment, and provide estimates of the potential human health and environmental risks associated with the background level concentrations of potentially hazardous constituents

  9. Project plan for the Background Soil Characterization Project on the Oak Ridge Reservation, Oak Ridge, Tennessee

    1992-08-01

    The Background Soil characterization Project (BSCP) will provide background concentration levels of selected metals, organic compounds, and radionuclides in soils from uncontaminated on-site areas at the Oak Ridge Reservation (ORR), and off-site in the western part of Roane County and the eastern part of Anderson County. The BSCP will establish a database, recommend how to use the data for contaminated site assessment, and provide estimates of the potential human health and environmental risks associated with the background level concentrations of potentially hazardous constituents.

  10. Cultural-resource survey report: Hoover Dam Powerplant Modification Project II. Associated transmission-line facility

    Queen, R.L.

    1991-06-01

    The Bureau of Reclamation (Reclamation) is proposing to modify or install additional transmission facilities between the Hoover Dam hydroelectric plant and the Western Area Power Authority substation near Boulder City, Nevada. Reclamation has completed cultural resource investigations to identify historic or prehistoric resources in the project area that might be affected during construction of the transmission line. Four possible transmission corridors approximately 50 feet wide and between 9.5 and 11.5 miles long were investigated. The proposed transmission lines either parallel or replace existing transmission lines. The corridors generally have undergone significant disturbance from past transmission line construction. A Class II sampling survey covering approximately 242 acres was conducted. Access or construction roads have not been identified and surveys of these areas will have to be completed in the future. No historic or prehistoric archeological sites were encountered within the four corridor right-of-ways. It is believed that the probability for prehistoric sites is very low. Four historic period sites were recorded that are outside, but near, the proposed corridor. These sites are not individually eligible for the National Register of Historic Places, but may be associated with the construction of Hoover Dam and contribute to a historic district or multiple property resource area focusing on the dam and its construction

  11. Metrological characterization methods for confocal chromatic line sensors and optical topography sensors

    Seppä, Jeremias; Niemelä, Karri; Lassila, Antti

    2018-05-01

    The increasing use of chromatic confocal technology for, e.g. fast, in-line optical topography, and measuring thickness, roughness and profiles implies a need for the characterization of various aspects of the sensors. Single-point, line and matrix versions of chromatic confocal technology, encoding depth information into wavelength, have been developed. Of these, line sensors are particularly suitable for in-line process measurement. Metrological characterization and development of practical methods for calibration and checking is needed for new optical methods and devices. Compared to, e.g. tactile methods, optical topography measurement techniques have limitations related to light wavelength and coherence, optical properties of the sample including reflectivity, specularity, roughness and colour, and definition of optical versus mechanical surfaces. In this work, metrological characterization methods for optical line sensors were developed for scale magnification and linearity, sensitivity to sample properties, and dynamic characteristics. An accurate depth scale calibration method using a single prototype groove depth sample was developed for a line sensor and validated with laser-interferometric sample tracking, attaining (sub)micrometre level or better than 0.1% scale accuracy. Furthermore, the effect of different surfaces and materials on the measurement and depth scale was studied, in particular slope angle, specularity and colour. In addition, dynamic performance, noise, lateral scale and resolution were measured using the developed methods. In the case of the LCI1200 sensor used in this study, which has a 11.3 mm  ×  2.8 mm measurement range, the instrument depth scale was found to depend only minimally on sample colour, whereas measuring steeply sloped specular surfaces in the peripheral measurement area, in the worst case, caused a somewhat larger relative sample-dependent change (1%) in scale.

  12. Yucca Mountain Site Characterization Project: Technical Data Catalog quarterly supplement

    NONE

    1994-03-31

    The March 21, 1993, Department of Energy (DOE)/Nuclear Regulatory Commission (NRC) Site-Specific Procedural Agreement for Geologic Repository Site Investigation and Characterization Program requires the DOE to develop and maintain a catalog of data which will be updated and provided to the NRC at least quarterly. This catalog is to include a description of the data; the time (date), place, and method of acquisition; and where it may be examined. The Yucca Mountain Site Characterization Project (YMP) Technical Data Catalog is published and distributed in accordance with the requirements of the Site-Specific Agreement. The YMP Technical Data Catalog is a report based on reference information contained in the YMP Automated Technical Data Tracking System (ATDT). The reference information is provided by Participants for data acquired or developed in support of the YMP. The Technical Data Catalog is updated quarterly and published in the month following the end of each quarter. A complete revision to the Catalog is published at the end of each fiscal year. Supplements to the end-of-year edition are published each quarter. These supplements provide information related to new data items not included in previous quarterly updates and data items affected by changes to previously published reference information. The Technical Data Catalog, dated September 30, 1993, should be retained as the baseline document for the supplements until the end-of-year revision is published and distributed in October 1994.

  13. Yucca Mountain Site Characterization Project Technical Data Catalog (quarterly supplement)

    1993-01-01

    The June 1, 1985, Department of Energy (DOE)/Nuclear Regulatory Commission (NRC) Site-Specific Procedural Agreement for Geologic Repository Site Investigation and Characterization Program requires the DOE to develop and maintain a catalog of data which will be updated and provided to the NRC at least quarterly. This catalog is to include a description of the data; the time (date), place, and method of acquisition; and where it may be examined. The Yucca Mountain Site Characterization Project (YMP) Technical Data Catalog is published and distributed in accordance with the requirements of the Site-Specific Agreement. The YMP Technical Data Catalog is a report based on reference information contained in the YMP Automated Technical Data Tracking System (ATDT). The reference information is provided by Participants for data acquired or developed in support of the YMP. The Technical Data Catalog is updated quarterly and published in the month following the end of each quarter. A complete revision to the Catalog is published at the end of each fiscal year. Supplements to the end-of-year edition are published each quarter. These supplements provide information related to new data items not included in previous quarterly updates and data items affected by changes to previously published reference information. The Technical Data Catalog, dated December 31, 1992, should be retained as the baseline document for the supplements until the end-of-year revision is published and distributed in October 1993

  14. Yucca Mountain Site Characterization Project Technical Data Catalog

    1992-01-01

    The June 1, 1985, Department of Energy (DOE)/Nuclear, Regulatory Commission (NRC) Site-Specific Procedural Agreement for Geologic Repository Site Investigation and Characterization Program requires the DOE to develop and maintain a catalog of data which will be updated and provided to the NRC at least quarterly. This catalog is to include a description of the data; the time (date), place, and method of acquisition; and where it may be examined. The Yucca Mountain Site Characterization Project (YMP) Technical Data Catalog is published and distributed in accordance with the requirements of the Site-Specific Agreement. The YMP Technical Data Catalog is a report based on reference information contained in the YMP Automated Technical Data Tracking System (ATDT). The reference information is provided by Participants for data acquired or developed in support of the YMP. The Technical Data Catalog is updated quarterly and published in the month following the end of each quarter. Each new publication of the Technical Data Catalog supersedes the previous edition

  15. Yucca Mountain Site Characterization Project technical data catalog

    1992-01-01

    The June 1, 1985, Department of Energy (DOE)/Nuclear Regulatory Commission (NRC) Site-Specific Procedural Agreement for Geologic Repository Site Investigation and Characterization Program requires the DOE to develop and maintain a catalog of data which will be updated and provided to the NRC at least quarterly. This catalog is to include a description of the data; the time (date), place, and method of acquisition; and where it may be examined. The Yucca Mountain Site Characterization Project (YMP) Technical Data Catalog is published and distributed in accordance with the requirements of the Site-Specific Agreement. The YMP Technical Data Catalog is a report based on reference information contained in the YMP Automated Technical Data Tracking System (ATDT). The reference information is provided by Participants for data acquired or developed in support of the YMP. The Technical Data Catalog is updated quarterly and published in the month following the end of each quarter. Each new publication of the Technical Data Catalog supersedes the previous edition

  16. Yucca Mountain Site Characterization Project technical data catalog: Quarterly supplement

    NONE

    1994-12-31

    The Department of Energy (DOE)/Nuclear Regulatory Commission (NRC) Site-Specific Procedural Agreement for Geologic Repository Site Investigation and Characterization Program requires the DOE to develop and maintain a catalog of data which will be updated and provided to the NRC at least quarterly. This catalog is to include a description of the data; the time (date), place, and method of acquisition; and where the data may be examined. The Yucca Mountain Site Characterization Project (YMP) Technical Data Catalog is published and distributed-in accordance with the requirements of the Site-Specific Agreement. The YMP Technical Data Catalog is a report based on reference information contained in the YMP Automated Technical Data Tracking System (ATDT). The reference information is provided by Participants for data acquired or developed in support of the YMP. The Technical Data Catalog is updated quarterly and distributed in the month following the end of each quarter. A complete revision to the catalog is published at the end of each fiscal year. Supplements to the end-of-year edition are published each quarter. These supplements provide information related to new data items not included in previous quarterly updates and data items affected by changes to previously published reference information. The Technical Data Catalog, dated September 30, 1994, should be retained as the baseline document for the supplements until the end-of-year revision is published and distributed in October 1995.

  17. Yucca Mountain Site Characterization Project exploratory studies facilities construction status

    Allan, J.N.; Leonard, T.M.

    1993-01-01

    This paper discusses the progress to date on the construction planning and development of the Yucca Mountain Site Characterization Project (YMP) Exploratory Studies Facilities (ESF). The purpose of the ESF is to determine early site suitability and to characterize the subsurface of the Yucca Mountain site to assess its suitability for a potential high level nuclear waste repository. The present ESF configuration concept is for two main ramps to be excavated by tunnel boring machines (TBM) from the surface to the Topopah Spring Member of the Paintbrush Tuff Formation. From the main ramps, slightly above Topopah Spring level, supplemental ramps will be penetrated to the Calico Hills formation below the potential repository. There will be exploratory development drifts driven on both levels with the Main Test Area being located on the Topopah Spring level, which is the level of the proposed repository. The Calico Hills formation lies below the Topopah Spring member and is expected to provide the main geo-hydrologic barrier between the potential repository and the underlying saturated zones in the Crater Flat Tuff

  18. A characterization of linearly repetitive cut and project sets

    Haynes, Alan; Koivusalo, Henna; Walton, James

    2018-02-01

    For the development of a mathematical theory which can be used to rigorously investigate physical properties of quasicrystals, it is necessary to understand regularity of patterns in special classes of aperiodic point sets in Euclidean space. In one dimension, prototypical mathematical models for quasicrystals are provided by Sturmian sequences and by point sets generated by substitution rules. Regularity properties of such sets are well understood, thanks mostly to well known results by Morse and Hedlund, and physicists have used this understanding to study one dimensional random Schrödinger operators and lattice gas models. A key fact which plays an important role in these problems is the existence of a subadditive ergodic theorem, which is guaranteed when the corresponding point set is linearly repetitive. In this paper we extend the one-dimensional model to cut and project sets, which generalize Sturmian sequences in higher dimensions, and which are frequently used in mathematical and physical literature as models for higher dimensional quasicrystals. By using a combination of algebraic, geometric, and dynamical techniques, together with input from higher dimensional Diophantine approximation, we give a complete characterization of all linearly repetitive cut and project sets with cubical windows. We also prove that these are precisely the collection of such sets which satisfy subadditive ergodic theorems. The results are explicit enough to allow us to apply them to known classical models, and to construct linearly repetitive cut and project sets in all pairs of dimensions and codimensions in which they exist. Research supported by EPSRC grants EP/L001462, EP/J00149X, EP/M023540. HK also gratefully acknowledges the support of the Osk. Huttunen foundation.

  19. Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

    Wang, Xin-Ying; Lai, Zhou-Sheng; Yeung, Chung-Man; Wang, Ji-De; Deng, Wen; Li, Hoi Yee; Han, Yu-Jing; Kung, Hsiang-Fu; Jiang, Bo; Lin, Marie Chia-mi

    2008-01-01

    AIM: To study the molecular mechanism of laterally spreading tumor (LST), a cell line [Laterally Spreading Tumor-Rectum 1 (LST-R1)] was derived and the characteristics of this cell line were investigated. METHODS: A new cell line (LST-R1) originated from laterally spreading tumor was established. Properties of the cell line were characterized using scanning and transmission electron microscopy, immunohistochemistry method, cytogenetic analysis and nude mice xenograft experiments. In vitro invasion assay, cDNA microarray and Western blotting were used to compare the difference between the LST-R1 and other colorectal cancer cell lines derived from prudent colon cancer. RESULTS: Our study demonstrated that both epithelial special antigen (ESA) and cytokeratin-20 (CK20) were expressed in LST-R1. The cells presented microvilli and tight junction with large nuclei. The karyotypic analysis showed hyperdiploid features with structural chromosome aberrations. The in vivo tumorigenicity was also demonstrated in nude mice xenograft experiments. The invasion assay suggested this cell line has a higher invasive ability. cDNA microarray and Western blotting show the loss of the expression of E-cadherin in LST-R1 cells. CONCLUSION: We established and characterized a colorectal cancer cell line, LST-R1 and LST-R1 has an obvious malignant tendency, which maybe partially attributed to the changes of the expression of some adhesion molecules, such as E-cadherin. It is also a versatile tool for exploring the original and progressive mechanisms of laterally spreading tumor and the early colon cancer genesis. PMID:18300345

  20. Characterization of cell lines developed from the glassy-winged sharpshooter, Homalodisca coagulata (Hemiptera: Cicadellidae).

    Kamita, Shizuo G; Do, Zung N; Samra, Aman I; Hagler, James R; Hammock, Bruce D

    2005-01-01

    Four continuous cell lines were established from the embryos of the glassy-winged sharpshooter, Homalodisca coagulata (Say), an economically important insect vector of bacterial pathogens of grape, almond, citrus, oleander, and other agricultural and ornamental plantings. The cell lines were designated GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH. The GWSS-Z10, GWSS-Z15, and GWSS-G3 lines were cultured in Ex-Cell 401 medium supplemented with 10% fetal bovine serum (FBS), whereas the GWSS-LH line was cultured in LH medium supplemented with 20% FBS. The cell lines were characterized in terms of their morphology, growth, protein composition, and polymerase chain reaction- amplification patterns of their chromosomal deoxyribonucleic acid. The population doubling times of GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH were 46.2, 90.9, 100.3, and 60.2 h, respectively. These lines should be useful for the study of insect-pathogenic viruses of leafhoppers, aphids, treehoppers, and other related insects as well as plant-pathogenic viruses that are transmitted by these insects.

  1. [Establishment and characterization of a cell line derived from human ovarian mucinous cystadenocarcinoma].

    Wan, Q; Xu, D; Li, Z

    2001-07-01

    To establish a cell line of human ovarian cancer, and study its characterization. The cell line was established by the cultivation of subsides walls, and kept by freezing. The morphology was observed by microscope and electromicroscope. The authors studied its growth and propagation, the agglutination test of phytohemagglutinin (PHA), the chromosome analysis, heterotransplanting, immuno-histochemistry staining, the analysis of hormone, the pollution examination and the test of sensitivity to virus etc. A new human ovarian carcinoma cell line, designated ovarian mucinous cystadenocarcinoma 685 (OMC685), was established from mucinous cystadenocarcinoma. This cell line had subcultured to 91 generations, and some had been frozen for 8 years and revived, still grew well. This cell line possessed the feature of glandular epithelium cancer cell. The cells grew exuberantly, and the agglutinating test of PHA was positive. Karyotype was subtriploid with distortion. Heterotransplantations, alcian blue periobic acid-schiff (AbPAS), mucicarmine, alcian blue stainings, estradiol (E2) and progesterone were all positive. Without being polluted, it was sensitive to polivirus-I, adenovirus 7 and measles virus. OMC685 is a distinct human ovarian tumous cell line.

  2. Isolation and characterization of active LINE and SINEs from the eel.

    Kajikawa, Masaki; Ichiyanagi, Kenji; Tanaka, Nozomu; Okada, Norihiro

    2005-03-01

    Long interspersed elements (LINEs) and short interspersed elements (SINEs) are retrotransposons. These elements can mobilize by the "copy-and-paste" mechanism, in which their own RNA is reverse-transcribed into complementary DNA (cDNA). LINEs and SINEs not only are components of eukaryotic genomes but also drivers of genomic evolution. Thus, studies of the amplification mechanism of LINEs and SINEs are important for understanding eukaryotic genome evolution. Here we report the characterization of one LINE family (UnaL2) and two SINE families (UnaSINE1 and UnaSINE2) from the eel (Anguilla japonica) genome. UnaL2 is approximately 3.6 kilobases (kb) and encodes only one open reading frame (ORF). UnaL2 belongs to the stringent type--thought to be a major group of LINEs--and can mobilize in HeLa cells. We also show that UnaL2 and the two UnaSINEs have similar 3' tails, and that both UnaSINE1 and UnaSINE2 can be mobilized by UnaL2 in HeLa cells. These elements are thus useful for delineating the amplification mechanism of stringent type LINEs as well as that of SINEs.

  3. Establishment of a novel human medulloblastoma cell line characterized by highly aggressive stem-like cells.

    Silva, Patrícia Benites Gonçalves da; Rodini, Carolina Oliveira; Kaid, Carolini; Nakahata, Adriana Miti; Pereira, Márcia Cristina Leite; Matushita, Hamilton; Costa, Silvia Souza da; Okamoto, Oswaldo Keith

    2016-08-01

    Medulloblastoma is a highly aggressive brain tumor and one of the leading causes of morbidity and mortality related to childhood cancer. These tumors display differential ability to metastasize and respond to treatment, which reflects their high degree of heterogeneity at the genetic and molecular levels. Such heterogeneity of medulloblastoma brings an additional challenge to the understanding of its physiopathology and impacts the development of new therapeutic strategies. This translational effort has been the focus of most pre-clinical studies which invariably employ experimental models using human tumor cell lines. Nonetheless, compared to other cancers, relatively few cell lines of human medulloblastoma are available in central repositories, partly due to the rarity of these tumors and to the intrinsic difficulties in establishing continuous cell lines from pediatric brain tumors. Here, we report the establishment of a new human medulloblastoma cell line which, in comparison with the commonly used and well-established cell line Daoy, is characterized by enhanced proliferation and invasion capabilities, stem cell properties, increased chemoresistance, tumorigenicity in an orthotopic metastatic model, replication of original medulloblastoma behavior in vivo, strong chromosome structural instability and deregulation of genes involved in neural development. These features are advantageous for designing biologically relevant experimental models in clinically oriented studies, making this novel cell line, named USP-13-Med, instrumental for the study of medulloblastoma biology and treatment.

  4. Waste management of Line Item projects at Oak Ridge National Laboratory

    Zill, D.S.

    1993-01-01

    With the growing number of companies involved with today's Line Item projects at the Oak Ridge National Laboratory (ORNL), there are ever increasing problems in the handling of Radioactive Solid Low-Level Waste (SLLW). The most important of these problems is who is going to do what with the waste and when are they going to do it. The who brings to mind training; the what, compliance; and the when, cost. At ORNL, the authors have found that the best way to address the challenges of waste handling where several contractors are involved is through communication, compromise and consistency. Without these elements, opportunities bred from waste handling are likely to bring the project to a halt

  5. Establishment and characterization of a unique 1 microm diameter liver-derived progenitor cell line.

    Aravalli, Rajagopal N; Behnan Sahin, M; Cressman, Erik N K; Steer, Clifford J

    2010-01-01

    Liver-derived progenitor cells (LDPCs) are recently identified novel stem/progenitor cells from healthy, unmanipulated adult rat livers. They are distinct from other known liver stem/progenitor cells such as the oval cells. In this study, we have generated a LDPC cell line RA1 by overexpressing the simian virus 40 (SV40) large T antigen (TAg) in primary LDPCs. This cell line was propagated continuously for 55 passages in culture, after which it became senescent. Interestingly, following transformation with SV40 TAg, LDPCs decreased in size significantly and the propagating cells measured 1 microm in diameter. RA1 cells proliferated in vitro with a doubling time of 5-7 days, and expressed cell surface markers of LDPCs. In this report, we describe the characterization of this novel progenitor cell line that might serve as a valuable model to study liver cell functions and stem cell origin of liver cancers. Copyright 2009 Elsevier Inc. All rights reserved.

  6. Metabolic characterization of invaded cells of the pancreatic cancer cell line, PANC?1

    Fujita, Mayumi; Imadome, Kaori; Imai, Takashi

    2017-01-01

    We previously reported that about 0.4% of cells in the cultured human pancreatic cancer cell line, PANC?1, can invade matrigel during the transwell invasion assay, suggesting that these invaded PANC?1 cells may have specific characteristics to keep their invasive potential. To identify the metabolic characterization specific in the invaded PANC?1 cells, metabolome analysis of the invaded PANC?1 compared with the whole cultured PANC?1 was performed using CE?TOFMS, and concentrations of 110 met...

  7. Genetic and Genomic Characterization of 462 Melanoma Patient-Derived Xenografts, Tumor Biopsies, and Cell Lines

    Bradley Garman

    2017-11-01

    Full Text Available Summary: Tumor-sequencing studies have revealed the widespread genetic diversity of melanoma. Sequencing of 108 genes previously implicated in melanomagenesis was performed on 462 patient-derived xenografts (PDXs, cell lines, and tumors to identify mutational and copy number aberrations. Samples came from 371 unique individuals: 263 were naive to treatment, and 108 were previously treated with targeted therapy (34, immunotherapy (54, or both (20. Models of all previously reported major melanoma subtypes (BRAF, NRAS, NF1, KIT, and WT/WT/WT were identified. Multiple minor melanoma subtypes were also recapitulated, including melanomas with multiple activating mutations in the MAPK-signaling pathway and chromatin-remodeling gene mutations. These well-characterized melanoma PDXs and cell lines can be used not only as reagents for a large array of biological studies but also as pre-clinical models to facilitate drug development. : Garman et al. have characterized melanoma PDXs and cell lines described in Krepler et al. (see the related paper in this issue of Cell Reports, identifying major and minor subtypes, some of which were previously not well defined, targeted and immunotherapy resistance, and tumor heterogeneity, creating a set of reagents for future drug discovery and biological studies. Keywords: melanoma, patient-derived xenografts, massively parallel sequencing, cell lines

  8. THE BC CRIBS & TRENCHES GEOPHYSICAL CHARACTERIZATION PROJECT ONE STEP FORWARD IN HANFORDS CLEANUP PROCESS

    BENECKE, MN.W.

    2006-02-22

    A geophysical characterization project was conducted at the BC Cribs and Trenches Area, located south of 200 East at the Hanford Site. The area consists of 26 waste disposal trenches and cribs, which received approximately 30 million gallons of liquid waste from the uranium recovery process and the ferrocyanide processes associated with wastes generated by reprocessing nuclear fuel. Waste discharges to BC Cribs contributed perhaps the largest liquid fraction of contaminants to the ground in the 200 Areas. The site also includes possibly the largest inventory of Tc-99 ever disposed to the soil at Hanford with an estimated quantity of 400 Ci. Other waste constituents included high volumes of nitrate and U-238. The geophysical characterization at the 50 acre site primarily included high resolution resistivity (HRR). The resistivity technique is a non-invasive method by which electrical resistivity data are collected along linear transects, and data are presented as continuous profiles of subsurface electrical properties. The transects ranged in size from about 400-700 meters and provided information down to depths of 60 meters. The site was characterized by a network of 51 HRR lines with a total of approximately 19.7 line kilometers of data collected parallel and perpendicular to the trenches and cribs. The data were compiled to form a three-dimensional representation of low resistivity values. Low resistivity, or high conductivity, is indicative of high ionic strength soil and porewater resulting from the migration of nitrate and other inorganic constituents through the vadose zone. High spatial density soil data from a single borehole, that included coincident nitrate concentrations, electrical conductivity, and Tc-99, were used to transform the electrical resistivity data into a nitrate plume. The plume was shown to extend laterally beyond the original boundaries of the waste site and, in one area, to depths that exceeded the characterization strategy. It is

  9. THE BC CRIBS and TRENCHES GEOPHYSICAL CHARACTERIZATION PROJECT: ONE STEP FORWARD IN HANFORD'S CLEANUP PROCESS

    BENECKE, M.W.

    2005-01-01

    A geophysical characterization project was conducted at the BC Cribs and Trenches Area, located south of 200 East at the Hanford Site. The area consists of 26 waste disposal trenches and cribs, which received approximately 30 million gallons of liquid waste from the uranium recovery process and the ferrocyanide processes associated with wastes generated by reprocessing nuclear fuel. Waste discharges to BC Cribs contributed perhaps the largest liquid fraction of contaminants to the ground in the 200 Areas. The site also includes possibly the largest inventory of Tc-99 ever disposed to the soil at Hanford with an estimated quantity of 400 Ci. Other waste constituents included high volumes of nitrate and U-238. The geophysical characterization at the 50-acre site primarily included high resolution resistivity (HRR). The resistivity technique is a non-invasive method by which electrical resistivity data are collected along linear transects, and data are presented as continuous profiles of subsurface electrical properties. The transects ranged in size from about 400-700 meters and provided information down to depths of 60 meters. The site was characterized by a network of 51 HRR lines with a total of approximately 19.7 line kilometers of data collected parallel and perpendicular to the trenches and cribs. The data were compiled to form a three-dimensional representation of low resistivity values. Low resistivity, or high conductivity, is indicative of high ionic strength soil and porewater resulting from the migration of nitrate and other inorganic constituents through the vadose zone. High spatial density soil data from a single borehole, that included coincident nitrate concentrations, electrical conductivity. and Tc-99, were used to transform the electrical resistivity data into a nitrate plume. The plume was shown to extend laterally beyond the original boundaries of the waste site and, in one area, to depths that exceeded the characterization strategy

  10. Discussion after implementation of possible ergonomics contributions in production lines automation project

    José Adriano Canton

    2016-11-01

    Full Text Available This paper addresses the issue of work spaces designed based on Activity-Centered Ergonomics. The aim was to reflect on the role of Ergonomics in the design of productive situations in order to anticipate and mitigate the emergence of new constraints. This study was developed using a case study from the packaging industry, where an automated line was implemented to mitigate the injuries suffered as result of the manual system. This article was based on the results of an Ergonomic Work Analysis of the solution implemented, and the subsequent analysis of this design process. It was found that the adopted solution does not have enough leeway for dealing with variations inherent to the process, requiring constant interventions in the operation, by the operators in order to free the line to continue work. It was evident that the adopted solution did not involve the participation of important agents such as the operators and the maintenance crew, giving the project a technical vision that was concerned with eliminating the human factor. The conclusion of this study was that the Ergonomics integrated into the project development favors the collective construction of analysis of the research, and consequently, the development of more effective solutions.

  11. Direct/Delayed Response Project: Soil-characterization comparison

    Fenstermaker, L.K.; Byers, G.E.; Starks, T.H.; Miah, M.J.; Palmer, C.J.

    1992-01-01

    A large amount of soil characterization data has been collected as a component of the Direct/Delayed Response Project (DDRP) in the acid rain Aquatic Effects Research Program. An interlaboratory comparison study was undertaken to identify the comparability of the data to that obtained from representative soil characterization laboratories. Participating laboratories were selected at random from four regions of the U.S. and two regions of Canada. Two original DDRP contract laboratories also participated. Duplicate samples of six soil audit materials and two liquid soil extracts were sent to each of the laboratories in two separate batches. Laboratories used their own protocols to perform the analyses requested except for the contract laboratories which followed the DDRP protocol. Liquid audits were used in an effort to identify if interlaboratory differences were due to extraction procedures or chemical measurements. A component of the variability in the results was attributed to differences in the methods used such as soil/solution ratios, extractants or extraction procedures. The largest number of different methods used was for the measurement of cation exchange capacity. The results between the DDRP soil survey data and the study's results were compared using Youden-pair plots. In addition, standard statistical tests were performed. Overall, the DDRP data were comparable to the data from the study. However, out of the total 141 comparisons involving results from six or more laboratories, the results from the two contract laboratories did not meet the comparison criteria in 19 cases. Since there was never a case in which both contract laboratories failed, it would appear that the 19 cases which were not comparable were due to random analytical errors, incorrectly reported results, or misapplication of DDRP protocol

  12. Canby Area Service Project substation and associated transmission line. Environmental Assessment

    1992-02-01

    Bonneville Power Administration (BPA) provides power to Surprise Valley Electrification Corporation (SVEC) in Modoc County, California. BPA uses PacificCorp`s substation and transmission facilities between Alturas and Canby, California to transfer power to SVEC`s Canby Substation. In the next year, SVEC expects increased industrial, agricultural, and residential electric loads on their 69-kV transmission system south of Canby. SVEC`s substation can accommodate only about 10 percent of the expected additional electric load. BPA`s proposed action is intended to meet SVEC`s increasing electric load. BPA proposes to meet SVEC`s increasing energy load by tapping into BPA`s existing BPA Malin-Warner 230-kV transmission line, and building an 7.9-mile transmission line to a new BPA substation. BPA proposes to build the new substation next to the west side of SVEC`s Canby Substation (Figure 1). This new substation will allow SVEC to move the additional power over their existing transmission or distribution lines. This report is the environmental assessment of the potential impact of the proposed project. The assessment determined that no ``environmental impact statement`` is not required.

  13. Canby Area Service Project : Substation and Associated Transmission Line : Environmental Assessment.

    United States. Bonneville Power Administration.

    1992-02-01

    Bonneville Power Administration (BPA) provides power to Surprise Valley Electrification Corporation (SVEC) in Modoc County, California. BPA uses PacificCorp's substation and transmission facilities between Alturas and Canby, California to transfer power to SVEC's Canby Substation. In the next year, SVEC expects increased industrial, agricultural, and residential electric loads on their 69-kV transmission system south of Canby. SVEC's substation can accommodate only about 10 percent of the expected additional electric load. BPA's proposed action is intended to meet SVEC's increasing electric load. BPA proposes to meet SVEC's increasing energy load by tapping into BPA's existing BPA Malin-Warner 230-kV transmission line, and building an 7.9-mile transmission line to a new BPA substation. BPA proposes to build the new substation next to the west side of SVEC's Canby Substation (Figure 1). This new substation will allow SVEC to move the additional power over their existing transmission or distribution lines. This report is the environmental assessment of the potential impact of the proposed project. The assessment determined that no environmental impact statement'' is not required.

  14. THE SLOAN DIGITAL SKY SURVEY REVERBERATION MAPPING PROJECT: RAPID C iv BROAD ABSORPTION LINE VARIABILITY

    Grier, C. J.; Brandt, W. N.; Trump, J. R.; Schneider, D. P.; Hall, P. B.; Shen, Yue; Vivek, M.; Dawson, K. S.; Ak, N. Filiz; Chen, Yuguang; Denney, K. D.; Kochanek, C. S.; Peterson, B. M.; Green, Paul J.; Jiang, Linhua; McGreer, Ian D.; Pâris, I.; Tao, Charling; Wood-Vasey, W. M.; Bizyaev, Dmitry

    2015-01-01

    We report the discovery of rapid variations of a high-velocity C iv broad absorption line trough in the quasar SDSS J141007.74+541203.3. This object was intensively observed in 2014 as a part of the Sloan Digital Sky Survey Reverberation Mapping Project, during which 32 epochs of spectroscopy were obtained with the Baryon Oscillation Spectroscopic Survey spectrograph. We observe significant (>4σ) variability in the equivalent width (EW) of the broad (∼4000 km s −1 wide) C iv trough on rest-frame timescales as short as 1.20 days (∼29 hr), the shortest broad absorption line variability timescale yet reported. The EW varied by ∼10% on these short timescales, and by about a factor of two over the duration of the campaign. We evaluate several potential causes of the variability, concluding that the most likely cause is a rapid response to changes in the incident ionizing continuum. If the outflow is at a radius where the recombination rate is higher than the ionization rate, the timescale of variability places a lower limit on the density of the absorbing gas of n e ≳ 3.9 × 10 5 cm −3 . The broad absorption line variability characteristics of this quasar are consistent with those observed in previous studies of quasars, indicating that such short-term variability may in fact be common and thus can be used to learn about outflow characteristics and contributions to quasar/host-galaxy feedback scenarios

  15. Characterization of cloned cells from an immortalized fetal pulmonary type II cell line

    Henderson, R.F.; Waide, J.J.; Lechner, J.F.

    1995-12-01

    A cultured cell line that maintained expression of pulmonary type II cell markers of differentiation would be advantageous to generate a large number of homogenous cells in which to study the biochemical functions of type II cells. Type II epithelial cells are the source of pulmonary surfactant and a cell of origin for pulmonary adenomas. Last year our laboratory reported the induction of expression of two phenotypic markers of pulmonary type II cells (alkaline phosphatase activity and surfactant lipid synthesis) in cultured fetal rat lung epithelial (FRLE) cells, a spontaneously immortalized cell line of fetal rat lung type II cell origin. Subsequently, the induction of the ability to synthesize surfactant lipid became difficult to repeat. We hypothesized that the cell line was heterogenuous and some cells were more like type II cells than others. The purpose of this study was to test this hypothesis and to obtain a cultured cell line with type II cell phenotypic markers by cloning several FRLE cells and characterizing them for phenotypic markers of type II cells (alkaline phosphatase activity and presence of surfactant lipids). Thirty cloned cell lines were analyzed for induced alkaline phosphatase activity (on x-axis) and for percent of phospholipids that were disaturated (i.e., surfactant).

  16. Development and characterization of two cell lines from gills of Atlantic salmon

    Gjessing, Mona C.; Aamelfot, Maria; Batts, William N.; Benestad, Sylvie L.; Dale, Ole B.; Thoen, Even; Weli, Simon C.; Winton, James R.

    2018-01-01

    Gill disease in Atlantic salmon, Salmo salar L., causes big losses in the salmon farming industry. Until now, tools to cultivate microorganisms causing gill disease and models to study the gill responses have been lacking. Here we describe the establishment and characterization of two cell lines from the gills of Atlantic salmon. Atlantic salmon gill cell ASG-10 consisted of cells staining for cytokeratin and e-cadherin and with desmosomes as seen by transmission electron microscopy suggesting the cells to be of epithelial origin. These structures were not seen in ASG-13. The cell lines have been maintained for almost 30 passages and both cell lines are fully susceptible to infection by infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), infectious pancreatic necrosis virus (IPNV), Atlantic salmon reovirus TS (TSRV) and Pacific salmon paramyxovirus (PSPV). While infectious salmon anemia virus (ISAV) did not cause visible CPE, immunofluorescent staining revealed a sub-fraction of cells in both the ASG-10 and ASG-13 lines may be permissive to infection. ASG-10 is able to proliferate and migrate to close scratches in the monolayer within seven days in vitro contrary to ASG-13, which does not appear to do have the same proliferative and migratory ability. These cell lines will be useful in studies of gill diseases in Atlantic salmon and may represent an important contribution for alternatives to experimental animals and studies of epithelial–mesenchymal cell biology.

  17. Architecture and Functionality of the Advanced Life Support On-Line Project Information System

    Hogan, John A.; Levri, Julie A.; Morrow, Rich; Cavazzoni, Jim; Rodriguez, Luis F.; Riano, Rebecca; Whitaker, Dawn R.

    2004-01-01

    An ongoing effort is underway at NASA Ames Research Center (ARC) to develop an On-line Project Information System (OPIS) for the Advanced Life Support (ALS) Program. The objective of this three-year project is to develop, test, revise and deploy OPIS to enhance the quality of decision-making metrics and attainment of Program goals through improved knowledge sharing. OPIS will centrally locate detailed project information solicited from investigators on an annual basis and make it readily accessible by the ALS Community via a Web-accessible interface. The data will be stored in an object-oriented relational database (created in MySQL) located on a secure server at NASA ARC. OPE will simultaneously serve several functions, including being an research and technology development (R&TD) status information hub that can potentially serve as the primary annual reporting mechanism for ALS-funded projects. Using OPIS, ALS managers and element leads will be able to carry out informed R&TD investment decisions, and allow analysts to perform accurate systems evaluations. Additionally, the range and specificity of information solicited will serve to educate technology developers of programmatic needs. OPIS will collect comprehensive information from all ALS projects as well as highly detailed information specific to technology development in each ALS area (Waste, Water, Air, Biomass, Food, Thermal, Controls and Systems Analysis). Because the scope of needed information can vary dramatically between areas, element-specific technology information is being compiled with the aid of multiple specialized working groups. This paper presents the current development status in terms of the architecture and functionality of OPIS. Possible implementation approaches for OPIS are also discussed.

  18. Development Approach of the Advanced Life Support On-line Project Information System

    Levri, Julie A.; Hogan, John A.; Morrow, Rich; Ho, Michael C.; Kaehms, Bob; Cavazzoni, Jim; Brodbeck, Christina A.; Whitaker, Dawn R.

    2005-01-01

    The Advanced Life Support (ALS) Program has recently accelerated an effort to develop an On-line Project Information System (OPIS) for research project and technology development data centralization and sharing. There has been significant advancement in the On-line Project Information System (OPIS) over the past year (Hogan et al, 2004). This paper presents the resultant OPIS development approach. OPIS is being built as an application framework consisting of an uderlying Linux/Apache/MySQL/PHP (LAMP) stack, and supporting class libraries that provides database abstraction and automatic code generation, simplifying the ongoing development and maintenance process. Such a development approach allows for quick adaptation to serve multiple Programs, although initial deployment is for an ALS module. OPIS core functionality will involve a Web-based annual solicitation of project and technology data directly from ALS Principal Investigators (PIs) through customized data collection forms. Data provided by PIs will be reviewed by a Technical Task Monitor (TTM) before posting the information to OPIS for ALS Community viewing via the Web. Such Annual Reports will be permanent, citable references within OPIS. OPlS core functionality will also include Project Home Sites, which will allow PIS to provide updated technology information to the Community in between Annual Report updates. All data will be stored in an object-oriented relational database, created in MySQL(Reistered Trademark) and located on a secure server at NASA Ames Research Center (ARC). Upon launch, OPlS can be utilized by Managers to identify research and technology development (R&TD) gaps and to assess task performance. Analysts can employ OPlS to obtain the current, comprehensive, accurate information about advanced technologies that is required to perform trade studies of various life support system options. ALS researchers and technology developers can use OPlS to achieve an improved understanding of the NASA

  19. Characterization of immortalized MARCO and SR-AI/II-deficient murine alveolar macrophage cell lines

    Imrich Amy

    2008-05-01

    Full Text Available Abstract Background Alveolar macrophages (AM avidly bind and ingest unopsonized inhaled particles and bacteria through class A scavenger receptors (SRAs MARCO and SR-AI/II. Studies to characterize the function of these SRAs have used AMs from MARCO or SR-AI/II null mice, but this approach is limited by the relatively low yield of AMs. Moreover, studies using both MARCO and SR-AI/II-deficient (MS-/- mice have not been reported yet. Hence, we sought to develop continuous cell lines from primary alveolar macrophages from MS-/- mice. Results We used in vitro infection of the primary AMs with the J2 retrovirus carrying the v-raf and v-myc oncogenes. Following initial isolation in media supplemented with murine macrophage colony-stimulating factor (M-CSF, we subcloned three AM cell lines, designated ZK-1, ZK-2 and ZK-6. These cell lines grow well in RPMI-1640-10% FBS in the absence of M-CSF. These adherent but trypsin-sensitive cell lines have a doubling time of approximately 14 hours, exhibit typical macrophage morphology, and express macrophage-associated cell surface Mac-1 (CD11b and F4/80 antigens. The cell lines show robust Fc-receptor dependent phagocytosis of opsonized red blood cells. Similar to freshly isolated AMs from MS-/- mice, the cell lines exhibit decreased phagocytosis of unopsonized titanium dioxide (TiO2, fluorescent latex beads and bacteria (Staphylococcus aureus compared with the primary AMs from wild type (WT C57BL/6 mice. Conclusion Our results indicated that three contiguous murine alveolar macrophage cell lines with MS-/- (ZK1, ZK2 and ZK6 were established successfully. These cell lines demonstrated macrophage morphology and functional activity. Interestingly, similar to freshly isolated AMs from MS-/- mice, the cell lines have a reduced, but not absent, ability to bind and ingest particles, with an altered pattern of blockade by scavenger receptor inhibitors. These cell lines will facilitate in vitro studies to further define

  20. Letter of Intent for River Protection Project (RPP) Characterization Program: Process Engineering and Hanford Analytical Services and Characterization Project Operations and Quality Assurance

    ADAMS, M.R.

    2000-01-01

    The Characterization Project level of success achieved by the River Protection Project (RPP) is determined by the effectiveness of several organizations across RPP working together. The requirements, expectations, interrelationships, and performance criteria for each of these organizations were examined in order to understand the performances necessary to achieve characterization objectives. This Letter of Intent documents the results of the above examination. It formalizes the details of interfaces, working agreements, and requirements for obtaining and transferring tank waste samples from the Tank Farm System (RPP Process Engineering, Characterization Project Operations, and RPP Quality Assurance) to the characterization laboratory complex (222-S Laboratory, Waste Sampling and Characterization Facility, and the Hanford Analytical Service Program) and for the laboratory complex analysis and reporting of analytical results

  1. Characterization of appendage weld quality by on line monitoring of electrical parameters

    Setty, D.S.; Somani, A.K.; Ram, A.M.; Rao, A.R.; Jayaraj, R.N.; Kalidas, R. [Nuclear Fuel Complex, Dept. of Atomic Energy, Hyderabad (India)

    2005-07-01

    Resistance projection welding of zirconium alloy appendages is one of the most critical processes in the PHWR fuel fabrication. Appendages like Spacers and Bearing pads having multi projections are joined to the fuel sheath using capacitor discharge power source. Variations in the projection sizes, weld parameters and cleanliness of the work pieces have significant effect on the weld quality, in addition to material properties like hardness, tensile strength and surface finish. Defects like metal expulsion and weak welds are occasionally observed in appendage welding process, which need to be identified and segregated. Though numerous off-line inspection methods are available for the weld quality evaluation, on-line monitoring of weld quality is essential for identifying defective welds. For this purpose, various monitoring techniques like acoustic emission, analyzing derived electrical parameters and weld upset/deformation measurements are employed. The derived electrical parameters like A{sup 2}-Sec and Ohm-Sec can also be monitored. The present paper highlights development of suitable acceptance criteria for the monitoring technique by employing derived electrical parameters covering a wide range of weld variables like watt-sec and squeeze force. Excellent correlation could be achieved in identifying the weak welds and weld expulsion defects in mass production. (author)

  2. Architecture and Functionality of the Advanced Life Support On-Line Project Information System (OPIS)

    Hogan, John A.; Levri, Julie A.; Morrow, Rich; Cavazzoni, Jim; Rodriquez, Luis F.; Riano, Rebecca; Whitaker, Dawn R.

    2004-01-01

    An ongoing effort is underway at NASA Amcs Research Center (ARC) tu develop an On-line Project Information System (OPIS) for the Advanced Life Support (ALS) Program. The objective of this three-year project is to develop, test, revise and deploy OPIS to enhance the quality of decision-making metrics and attainment of Program goals through improved knowledge sharing. OPIS will centrally locate detailed project information solicited from investigators on an annual basis and make it readily accessible by the ALS Community via a web-accessible interface. The data will be stored in an object-oriented relational database (created in MySQL(Trademark) located on a secure server at NASA ARC. OPE will simultaneously serve several functions, including being an R&TD status information hub that can potentially serve as the primary annual reporting mechanism. Using OPIS, ALS managers and element leads will be able to carry out informed research and technology development investment decisions, and allow analysts to perform accurate systems evaluations. Additionally, the range and specificity of information solicited will serve to educate technology developers of programmatic needs. OPIS will collect comprehensive information from all ALS projects as well as highly detailed information specific to technology development in each ALS area (Waste, Water, Air, Biomass, Food, Thermal, and Control). Because the scope of needed information can vary dramatically between areas, element-specific technology information is being compiled with the aid of multiple specialized working groups. This paper presents the current development status in terms of the architecture and functionality of OPIS. Possible implementation approaches for OPIS are also discussed.

  3. Characterizing subsurface water flow to artificial drain lines using fiber-optic distributed temperature sensing

    Shults, D.; Brooks, E. S.; Heinse, R.; Keller, C. K.

    2017-12-01

    Over the last several years growers have experienced increasingly wet spring conditions in the Palouse Region located in North Idaho, Eastern Washington and Eastern Oregon. As a result more artificial drain lines are being installed so growers can access their fields earlier in the growing season. Additionally there has been increasing adoption of no-tillage practices among growers in order minimize erosion and runoff in the region. There is a growing body of evidence that suggests long-term no-tillage may lead to the establishment of large macropore networks through increased earthworm activity and the preservation of root channels. These macropore networks, in conjunctions with the presence of artificial drains lines, may create connected preferential flow paths from agricultural fields to receiving streams. This connectivity of flow paths from agricultural fields to receiving water bodies may increase the loading of nutrients and agricultural chemicals as some flow paths may largely bypass soil matrix interaction where materials can be sequestered. Our primary objective for this study was to characterize subsurface flow to two artificial drain lines, one under conventional tillage and the other under no-tillage, using distributed temperature sensing (DTS) technology. During the study (November 2016-April 2017) the near surface soil-water temperature was consistently colder than that of deeper depths. Temperature was thus used as a tracer as snow melt and soil-water moved from the near surface to the drain lines during snowmelt and precipitation events. The spatial and temporal variability of the temperature along the artificial drain line under no-tillage practices was found to be greater than that of the conventional tilled field. It is hypothesized that preferential flow paths are responsible for the increased variability of temperature seen in the drain line under long term no-till management. The temperature along the conventional till drain line showed a

  4. Isolation and characterization of conditionally immortalized mouse glomerular endothelial cell lines.

    Rops, Angelique L; van der Vlag, Johan; Jacobs, Cor W; Dijkman, Henry B; Lensen, Joost F; Wijnhoven, Tessa J; van den Heuvel, Lambert P; van Kuppevelt, Toin H; Berden, Jo H

    2004-12-01

    The culture and establishment of glomerular cell lines has proven to be an important tool for the understanding of glomerular cell functions in glomerular physiology and pathology. Especially, the recent establishment of a conditionally immortalized visceral epithelial cell line has greatly boosted the research on podocyte biology. Glomeruli were isolated from H-2Kb-tsA58 transgenic mice that contain a gene encoding a temperature-sensitive variant of the SV40 large tumor antigen, facilitating proliferative growth at 33 degrees C and differentiation at 37 degrees C. Glomerular endothelial cells were isolated from glomerular outgrowth by magnetic beads loaded with CD31, CD105, GSL I-B4, and ULEX. Clonal cell lines were characterized by immunofluorescence staining with antibodies/lectins specific for markers of endothelial cells, podocytes, and mesangial cells. Putative glomerular endothelial cell lines were analyzed for (1) cytokine-induced expression of adhesion molecules; (2) tube formation on Matrigel coating; and (3) the presence of fenestrae. As judged by immunostaining for Wilms tumor-1, smooth muscle actin (SMA), podocalyxin, and von Willebrand factor (vWF), we obtained putative endothelial, podocyte and mesangial cell lines. The mouse glomerular endothelial cell clone #1 (mGEnC-1) was positive for vWF, podocalyxin, CD31, CD105, VE-cadherin, GSL I-B4, and ULEX, internalized acetylated-low-density lipoprotein (LDL), and showed increased expression of adhesion molecules after activation with proinflammatory cytokines. Furthermore, mGEnC-1 formed tubes and contained nondiaphragmed fenestrae. The mGEnC-1 represents a conditionally immortalized cell line with various characteristics of differentiated glomerular endothelial cells when cultured at 37 degrees C. Most important, mGEnC-1 contains nondiaphragmed fenestrae, which is a unique feature of glomerular endothelial cells.

  5. Project of the JINR heavy ion synchrotron on-line control system

    Glejbman, Eh.M.; Zhabitskij, V.M.; Ivanov, I.N.

    1983-01-01

    Description of the project of the JINR heavy ion synchrotron (HIS) on-line control system (OCS) which is a strong-focusing synchrotron designed for avarage energies, is given. Complete average stream of data from HIS constitutes approximately 500 byte/s, when operation cycle is 0.33 s. The structure of HIS OCS is a two-hierarchy system with the distributed processing and control, built using modular principle. The first, lower hierarchy level forms eight subsystems, each of them is oriented for automation of concrete functionally-technological system of the accelerator. The higher hierarchy is the central complex computer which is a multimicroprocessor computer. The hardware of HIS OCS is envisaged to be realized on the base of CAMAC moduls. HIS OCS software will be disigned as the SM computer specialized real-time system supplemented with applied programs and language interpreter for the accelerator control

  6. Design and manufacture of the RF power supply and RF transmission line for SANAEM project Prometheus

    Turemen, G.; Ogur, S.; Ahiska, F.; Yasatekin, B.; Cicek, E.; Ozbey, A.; Kilic, I.; Unel, G.; Alacakir, A.

    2017-08-01

    A 1-5 MeV proton beamline is being built by the Turkish Atomic Energy Authority in collaboration with a number of graduate students from different universities. The primary goal of the project, is to acquire the design ability and manufacturing capability of all the components locally. SPP will be an accelerator and beam diagnostics test facility and it will also serve the detector development community with its low beam current. This paper discusses the design and construction of the RF power supply and the RF transmission line components such as its waveguide converters and its circulator. Additionally low and high power RF test results are presented to compare the performances of the locally produced components to the commercially available ones.

  7. Ontario Hydro's environmental monitoring program for HV [high voltage] transmission line projects

    Braekevelt, P.N.

    1991-01-01

    Responsible monitoring and control of environmental impacts is key to obtaining future needed approvals for new high voltage (HV) transmission line projects. Ontario Hydro's environmental monitoring program was developed as a highly structured, self-imposed monitoring system to relieve government agencies of the responsibility of developing a similar external program. The goal was to be self-policing. The historical development, program structure, standards, priority ratings, documentation, communication and computerization of the program is described. The most effective way to minimize environmental impacts is to avoid sensitive features at the route selection stage, well before any construction takes place. The environmental monitoring program is based on the following blueprint: each crew member is responsible for environmental protection; environmental problems are to be resolved at the lowest level possible; potential concerns should be resolved before they become problems; known problems should be dealt with quickly to minimize impacts; team members should work cooperatively; and formal and regular communication is emphasized

  8. Site characterization report for the Basalt Waste Isolation Project

    1982-11-01

    This Site Characterization Report documents the results of the site screening process, the preliminary site characterization data, the technical issues that need to be addressed, and the plans for resolving these issues

  9. Genome characterization of the selected long- and short-sleep mouse lines.

    Dowell, Robin; Odell, Aaron; Richmond, Phillip; Malmer, Daniel; Halper-Stromberg, Eitan; Bennett, Beth; Larson, Colin; Leach, Sonia; Radcliffe, Richard A

    2016-12-01

    The Inbred Long- and Short-Sleep (ILS, ISS) mouse lines were selected for differences in acute ethanol sensitivity using the loss of righting response (LORR) as the selection trait. The lines show an over tenfold difference in LORR and, along with a recombinant inbred panel derived from them (the LXS), have been widely used to dissect the genetic underpinnings of acute ethanol sensitivity. Here we have sequenced the genomes of the ILS and ISS to investigate the DNA variants that contribute to their sensitivity difference. We identified ~2.7 million high-confidence SNPs and small indels and ~7000 structural variants between the lines; variants were found to occur in 6382 annotated genes. Using a hidden Markov model, we were able to reconstruct the genome-wide ancestry patterns of the eight inbred progenitor strains from which the ILS and ISS were derived, and found that quantitative trait loci that have been mapped for LORR were slightly enriched for DNA variants. Finally, by mapping and quantifying RNA-seq reads from the ILS and ISS to their strain-specific genomes rather than to the reference genome, we found a substantial improvement in a differential expression analysis between the lines. This work will help in identifying and characterizing the DNA sequence variants that contribute to the difference in ethanol sensitivity between the ILS and ISS and will also aid in accurate quantification of RNA-seq data generated from the LXS RIs.

  10. Production and characterization of femtosecond laser-written double line waveguides in heavy metal oxide glasses

    da Silva, Diego Silvério; Wetter, Niklaus Ursus; de Rossi, Wagner; Kassab, Luciana Reyes Pires; Samad, Ricardo Elgul

    2018-01-01

    We report the fabrication and characterization of double line waveguides directly written in tellurite and germanate glasses using a femtosecond laser delivering 30 μJ, 80 fs pulses at 4 kHz repetition rate. The double line waveguides produced presented internal losses inferior to 2.0 dB/cm. The output mode profile and the M2 measurements indicate multimodal guiding behavior. A better beam quality for the GeO2 - PbO waveguide was observed when compared with TeO2 - ZnO glass. Raman spectroscopy of the waveguides showed structural modification of the glassy network and indicates that a negative refractive index modification occurs at the focus of the laser beam, therefore allowing for light guiding in between two closely spaced laser written lines. The refractive index change at 632 nm is around 10-4, and the structural changes in the laser focal region of the writing, evaluated by Raman spectroscopy, corroborated our findings that these materials are potential candidates for optical waveguides and passive components. To the best of our knowledge, the two double line configuration demonstrated in the present work was not reported before for germanate or tellurite glasses.

  11. Characterizing performances of solder paste printing process at flexible manufacturing lines

    Siew, Jit Ping; Low, Heng Chin; Teoh, Ping Chow

    2015-01-01

    Solder paste printing (SPP) has been a challenge on printed circuit board (PCB) manufacturing, evident by the proliferation of solder paste inspection equipment, or substituted by rigorous non-value added activity of manual inspections. The objective of this study is to characterize the SPP performance of various products manufactured in flexible production lines with different equipment configurations, and determine areas for process improvement. The study began by collecting information on SPP performance relative to component placement (CP) process, and to the proportion of mixed products. Using a clustering algorithm to group similar elements together, SPP performance across all product-production line pairs are statistically modeled to discover the trend and the influential factors. The main findings are: (a) Ratio of overall dpku for CP and SPP processes are 2:1; (b) logistic regression models of SPP performance indicated that only effects of product-production line and solder paste printer configuration are significant; (c) PCB circuitry design with BGA components and single solder paste printer line configurations generated the highest monthly defects, with the highest variation in the latter

  12. Characterizing performances of solder paste printing process at flexible manufacturing lines

    Siew, Jit Ping; Low, Heng Chin [University of Science Malaysia, 11800 Minden, Penang (Malaysia); Teoh, Ping Chow [Wawasan Open University, 54 Jalan Sultan Ahmad Shah, 10050 Penang (Malaysia)

    2015-02-03

    Solder paste printing (SPP) has been a challenge on printed circuit board (PCB) manufacturing, evident by the proliferation of solder paste inspection equipment, or substituted by rigorous non-value added activity of manual inspections. The objective of this study is to characterize the SPP performance of various products manufactured in flexible production lines with different equipment configurations, and determine areas for process improvement. The study began by collecting information on SPP performance relative to component placement (CP) process, and to the proportion of mixed products. Using a clustering algorithm to group similar elements together, SPP performance across all product-production line pairs are statistically modeled to discover the trend and the influential factors. The main findings are: (a) Ratio of overall dpku for CP and SPP processes are 2:1; (b) logistic regression models of SPP performance indicated that only effects of product-production line and solder paste printer configuration are significant; (c) PCB circuitry design with BGA components and single solder paste printer line configurations generated the highest monthly defects, with the highest variation in the latter.

  13. Projection systems with a cut-off line for automotive applications

    Kloos, G.; Eichhorn, K.

    2005-08-01

    The lighting systems of a car provide a variety of challenges from the point of view of illumination science and technology. Engineering work in this field has to deal both with reflector and lens design as well as with opto-mechanical design and sensor technology. It has direct implications on traffic safety and the efficiency in which energy is used. Therefore, these systems are continuously improved and optimized. In this context, adaptive systems that we investigate for automotive applications gain increasing importance. The properties of the light distribution in the vicinity of the cut-off line are of key importance for the safe and efficient operation of automotive headlamps. An alternative approach is proposed to refine the description of these properties in an attempt to make it more quantitative. This description is intended to facilitate intercomparison between different systems and/or to study environmental influences on the cut-off line of a system under investigation. Designing projection systems it is necessary to take a delicate trade-off between efficiency, light-distribution characteristics, mechanical boundary conditions, and legal requirements into account. Considerations and results on optical properties of three-axial reflectors in dependence of layout parameters will be given. They can serve as a guideline for the optical workshop and for free-form optimization.

  14. THE SLOAN DIGITAL SKY SURVEY REVERBERATION MAPPING PROJECT: RAPID C iv BROAD ABSORPTION LINE VARIABILITY

    Grier, C. J.; Brandt, W. N.; Trump, J. R.; Schneider, D. P. [Department of Astronomy and Astrophysics and Institute for Gravitation and the Cosmos, The Pennsylvania State University, 525 Davey Laboratory, University Park, PA 16802 (United States); Hall, P. B. [Department of Physics and Astronomy, York University, Toronto, ON M3J 1P3 (Canada); Shen, Yue [Carnegie Observatories, 813 Santa Barbara Street, Pasadena, CA 91101 (United States); Vivek, M.; Dawson, K. S. [Department of Physics and Astronomy, University of Utah, Salt Lake City, UT 84112 (United States); Ak, N. Filiz [Faculty of Sciences, Department of Astronomy and Space Sciences, Erciyes University, 38039 Kayseri (Turkey); Chen, Yuguang [Department of Astronomy, School of Physics, Peking University, Beijing 100871 (China); Denney, K. D.; Kochanek, C. S.; Peterson, B. M. [Department of Astronomy, The Ohio State University, 140 West 18th Avenue, Columbus, OH 43210 (United States); Green, Paul J. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Jiang, Linhua [Kavli Institute for Astronomy and Astrophysics, Peking University, Beijing 100871 (China); McGreer, Ian D. [Steward Observatory, The University of Arizona, 933 North Cherry Avenue, Tucson, AZ 85721-0065 (United States); Pâris, I. [INAF-Osservatorio Astronomico di Trieste, Via G. B. Tiepolo 11, I-34131 Trieste (Italy); Tao, Charling [Centre de Physique des Particules de Marseille, Aix-Marseille Universite, CNRS /IN2P3, 163, avenue de Luminy, Case 902, F-13288 Marseille Cedex 09 (France); Wood-Vasey, W. M. [PITT PACC, Department of Physics and Astronomy, University of Pittsburgh, 3941 O’Hara Street, Pittsburgh, PA 15260 (United States); Bizyaev, Dmitry, E-mail: grier@psu.edu [Apache Point Observatory and New Mexico State University, P.O. Box 59, Sunspot, NM, 88349-0059 (United States); and others

    2015-06-10

    We report the discovery of rapid variations of a high-velocity C iv broad absorption line trough in the quasar SDSS J141007.74+541203.3. This object was intensively observed in 2014 as a part of the Sloan Digital Sky Survey Reverberation Mapping Project, during which 32 epochs of spectroscopy were obtained with the Baryon Oscillation Spectroscopic Survey spectrograph. We observe significant (>4σ) variability in the equivalent width (EW) of the broad (∼4000 km s{sup −1} wide) C iv trough on rest-frame timescales as short as 1.20 days (∼29 hr), the shortest broad absorption line variability timescale yet reported. The EW varied by ∼10% on these short timescales, and by about a factor of two over the duration of the campaign. We evaluate several potential causes of the variability, concluding that the most likely cause is a rapid response to changes in the incident ionizing continuum. If the outflow is at a radius where the recombination rate is higher than the ionization rate, the timescale of variability places a lower limit on the density of the absorbing gas of n{sub e} ≳ 3.9 × 10{sup 5} cm{sup −3}. The broad absorption line variability characteristics of this quasar are consistent with those observed in previous studies of quasars, indicating that such short-term variability may in fact be common and thus can be used to learn about outflow characteristics and contributions to quasar/host-galaxy feedback scenarios.

  15. Environmental Assessment of the Gering-Stegall 115-kV Transmission Line Consolidation Project, Scotts Bluff County, Nebraska

    NONE

    1995-05-01

    The Department of Energy (DOE), Western Area Power Administration (Western) proposes to consolidate segments of two transmission lines near the Gering Substation in Gering, Nebraska. The transmission lines are both located in Scotts Bluff County, Nebraska. The transmission lines are both located in Scotts Bluff County, Nebraska, within the city of Gering. Presently, there are three parallel 115-kilovolt (kV) transmission lines on separate rights-of-way (ROW) that terminate at the Gering Substation. The project would include dismantling the Archer-Gering wood-pole transmission line and rebuilding the remaining two lines on single-pole steel double circuit structures. The project would consolidate the Gering-Stegall North and Gering-Stegall South 115-kV transmission lines on to one ROW for a 1.33-mile segment between the Gering Substation and a point west of the Gering Landfill. All existing wood-pole H-frame structures would be removed, and the Gering-Stegall North and South ROWs abandoned. Western is responsible for the design, construction, operation, and maintenance of the line. Western prepared an environmental assessment (EA) that analyzed the potential environmental impacts of the proposed construction, operation, and maintenance of the 115-kV transmission line consolidation. Based on the analyses in the EA, the DOE finds that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act of 1969 (NEPA).

  16. Environmental Assessment of the Gering-Stegall 115-kV Transmission Line Consolidation Project, Scotts Bluff County, Nebraska

    1995-05-01

    The Department of Energy (DOE), Western Area Power Administration (Western) proposes to consolidate segments of two transmission lines near the Gering Substation in Gering, Nebraska. The transmission lines are both located in Scotts Bluff County, Nebraska. The transmission lines are both located in Scotts Bluff County, Nebraska, within the city of Gering. Presently, there are three parallel 115-kilovolt (kV) transmission lines on separate rights-of-way (ROW) that terminate at the Gering Substation. The project would include dismantling the Archer-Gering wood-pole transmission line and rebuilding the remaining two lines on single-pole steel double circuit structures. The project would consolidate the Gering-Stegall North and Gering-Stegall South 115-kV transmission lines on to one ROW for a 1.33-mile segment between the Gering Substation and a point west of the Gering Landfill. All existing wood-pole H-frame structures would be removed, and the Gering-Stegall North and South ROWs abandoned. Western is responsible for the design, construction, operation, and maintenance of the line. Western prepared an environmental assessment (EA) that analyzed the potential environmental impacts of the proposed construction, operation, and maintenance of the 115-kV transmission line consolidation. Based on the analyses in the EA, the DOE finds that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act of 1969 (NEPA)

  17. Establishment and characterization of a new cell line of canine inflammatory mammary cancer: IPC-366.

    Sara Caceres

    Full Text Available Canine inflammatory mammary cancer (IMC shares epidemiologic, histopathological and clinical characteristics with the disease in humans and has been proposed as a natural model for human inflammatory breast cancer (IBC. The aim of this study was to characterize a new cell line from IMC (IPC-366 for the comparative study of both IMC and IBC. Tumors cells from a female dog with clinical IMC were collected. The cells were grown under adherent conditions. The growth, cytological, ultrastructural and immunohistochemical (IHC characteristics of IPC-366 were evaluated. Ten female Balb/SCID mice were inoculated with IPC-366 cells to assess their tumorigenicity and metastatic potential. Chromosome aberration test and Karyotype revealed the presence of structural aberration, numerical and neutral rearrangements, demonstrating a chromosomal instability. Microscopic examination of tumor revealed an epithelial morphology with marked anysocytosis. Cytological and histological examination of smears and ultrathin sections by electron microscopy revealed that IPC-366 is formed by highly malignant large round or polygonal cells characterized by marked atypia and prominent nucleoli and frequent multinucleated cells. Some cells had cytoplasmic empty spaces covered by cytoplasmic membrane resembling capillary endothelial cells, a phenomenon that has been related to s vasculogenic mimicry. IHC characterization of IPC-366 was basal-like: epithelial cells (AE1/AE3+, CK14+, vimentin+, actin-, p63-, ER-, PR-, HER-2, E-cadherin, overexpressed COX-2 and high Ki-67 proliferation index (87.15 %. At 2 weeks after inoculating the IPC-366 cells, a tumor mass was found in 100 % of mice. At 4 weeks metastases in lung and lymph nodes were found. Xenograph tumors maintained the original IHC characteristics of the female dog tumor. In summary, the cell line IPC-366 is a fast growing malignant triple negative cell line model of inflammatory mammary carcinoma that can be used for the

  18. Molecular characterization of neoplastic and normal "sister" lymphoblastoid B-cell lines from chronic lymphocytic leukemia

    Lanemo Myhrinder, Anna; Hellqvist, Eva; Bergh, Ann-Charlotte

    2013-01-01

    Chronic lymphocytic leukemia (CLL) B-cells resemble self-renewing CD5 + B-cells carrying auto/xeno-antigen-reactive B-cell receptors (BCRs) and multiple innate pattern-recognition receptors, such as Toll-like receptors and scavenger receptors. Integration of signals from BCRs with multiple surface...... a comprehensive genotypic and phenotypic characterization of available CLL and normal B-cell-derived lymphoblastoid cell lines (LCLs) from the same individuals (n = 17). Authenticity and verification studies of CLL-patient origin were done by IGHV sequencing, fluorescence in situ hybridization (FISH) and DNA...

  19. Characterization of Neptunium Oxide Generated Using the HB-Line Phase II Flowsheet

    Duffey, J

    2003-08-29

    Approximately 98 grams of neptunium(IV) oxide (NpO{sub 2}) were produced at the Savannah River Technology Center (SRTC) for use in gas generation tests to support the neptunium stabilization program at the Savannah River Site (SRS). The NpO{sub 2} was produced according to the anticipated HB-Line flowsheet consisting of anion exchange, oxalate precipitation, filtration, and calcination. Characterization of the NpO{sub 2} product to be used in gas generation tests included bulk and tap density measurements, X-ray diffraction, particle size distribution, specific surface area measurements, and moisture analysis.

  20. Isolation, Characterization, and Establishment of Spontaneously Immortalized Cell Line HRPE-2S With Stem Cell Properties.

    Shams Najafabadi, Hoda; Soheili, Zahra-Soheila; Samiei, Shahram; Ahmadieh, Hamid; Ranaei Pirmardan, Ehsan; Masoumi, Maryam

    2017-10-01

    The retinal pigment epithelium is a monolayer of highly specialized pigmented cells located between the neural retina and the Bruch's membrane of the choroid. RPE cells play a crucial role in the maintenance and function of the underlying photoreceptors. This study introduces a spontaneously arising human retinal pigment epithelial cell line, HRPE-2S, which was isolated from primary RPE cell culture of 2 days old male donor. We characterized morphology and functional properties of the new cell line. The immortalized cell line was maintained in culture for more than 70 passages and 240 divisions. The average doubling time of the cells was approximately 22 h and got freezed at 26th passage. The cell line expressed RPE-specific markers RPE65 and cell junction protein ZO1 as an epithelial cell marker. It also expressed CHX10, PAX6, Nestin, SOX2 as stem and retinal progenitor cell markers. Ki67 as a marker of cell proliferation was expressed in all HRPE-2S cells. It represented typical epithelial cobblestone morphology and did not phenotypically change through several passages. Stem cell-like aggregations (neurospheres) were observed in SEM microscopy. The cells represented high mitotic index. They could be viable under hypoxic conditions and serum deprivation. According to functional studies, the cell line exhibited stem cell-like behaviors with particular emphasis on its self-renewal capacity. LDH isoenzymes expression pattern confirmed the same cellular source for both of the HRPE-2S cells and primary RPE cells. Characteristics of HRPE-2S cells promise it as an in vitro model for RPE stem cell-based researches. J. Cell. Physiol. 232: 2626-2640, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  1. Production and molecular characterization of bread wheat lines with reduced amount of α-type gliadins.

    Camerlengo, Francesco; Sestili, Francesco; Silvestri, Marco; Colaprico, Giuseppe; Margiotta, Benedetta; Ruggeri, Roberto; Lupi, Roberta; Masci, Stefania; Lafiandra, Domenico

    2017-12-19

    Among wheat gluten proteins, the α-type gliadins are the major responsible for celiac disease, an autoimmune disorder that affects about 1% of the world population. In fact, these proteins contain several toxic and immunogenic epitopes that trigger the onset of the disease. The α-type gliadins are a multigene family, encoded by genes located at the complex Gli-2 loci. Here, three bread wheat deletion lines (Gli-A2, Gli-D2 and Gli-A2/Gli-D2) at the Gli-2 loci were generated by the introgression in the bread wheat cultivar Pegaso of natural mutations, detected in different bread wheat cultivars. The molecular characterization of these lines allowed the isolation of 49 unique expressed genes coding α-type gliadins, that were assigned to each of the three Gli-2 loci. The number and the amount of α-type gliadin transcripts were drastically reduced in the deletion lines. In particular, the line Gli-A2/Gli-D2 contained only 12 active α-type gliadin genes (-75.6% respect to the cv. Pegaso) and a minor level of transcripts (-80% compared to cv. Pegaso). Compensatory pleiotropic effects were observed in the two other classes of gliadins (ω- and γ-gliadins) either at gene expression or protein levels. Although the comparative analysis of the deduced amino acid sequences highlighted the typical structural features of α-type gliadin proteins, substantial differences were displayed among the 49 proteins for the presence of toxic and immunogenic epitopes. The deletion line Gli-A2/Gli-D2 did not contain the 33-mer peptide, one of the major epitopes triggering the celiac disease, representing an interesting material to develop less "toxic" wheat varieties.

  2. Establishment and Biological Characterization of a Panel of Glioblastoma Multiforme (GBM) and GBM Variant Oncosphere Cell Lines.

    Binder, Zev A; Wilson, Kelli M; Salmasi, Vafi; Orr, Brent A; Eberhart, Charles G; Siu, I-Mei; Lim, Michael; Weingart, Jon D; Quinones-Hinojosa, Alfredo; Bettegowda, Chetan; Kassam, Amin B; Olivi, Alessandro; Brem, Henry; Riggins, Gregory J; Gallia, Gary L

    2016-01-01

    Human tumor cell lines form the basis of the majority of present day laboratory cancer research. These models are vital to studying the molecular biology of tumors and preclinical testing of new therapies. When compared to traditional adherent cell lines, suspension cell lines recapitulate the genetic profiles and histologic features of glioblastoma multiforme (GBM) with higher fidelity. Using a modified neural stem cell culture technique, here we report the characterization of GBM cell lines including GBM variants. Tumor tissue samples were obtained intra-operatively and cultured in neural stem cell conditions containing growth factors. Tumor lines were characterized in vitro using differentiation assays followed by immunostaining for lineage-specific markers. In vivo tumor formation was assayed by orthotopic injection in nude mice. Genetic uniqueness was confirmed via short tandem repeat (STR) DNA profiling. Thirteen oncosphere lines derived from GBM and GBM variants, including a GBM with PNET features and a GBM with oligodendroglioma component, were established. All unique lines showed distinct genetic profiles by STR profiling. The lines assayed demonstrated a range of in vitro growth rates. Multipotency was confirmed using in vitro differentiation. Tumor formation demonstrated histologic features consistent with high grade gliomas, including invasion, necrosis, abnormal vascularization, and high mitotic rate. Xenografts derived from the GBM variants maintained histopathological features of the primary tumors. We have generated and characterized GBM suspension lines derived from patients with GBMs and GBM variants. These oncosphere cell lines will expand the resources available for preclinical study.

  3. Ground Characterization Studies in Canakkale Pilot Site of LIQUEFACT Project

    Ozcep, F.; Oztoprak, S.; Aysal, N.; Bozbey, I.; Tezel, O.; Ozer, C.; Sargin, S.; Bekin, E.; Almasraf, M.; Cengiz Cinku, M.; Ozdemir, K.

    2017-12-01

    The our aim is to outline the ground characterisation studies in Canakkale test site. Study is based on the EU H2020 LIQUEFACT project entitled "Liquefact: Assessment and mitigation of liquefaction potential across Europe: a holistic approach to protect structures / infrastructures for improved resilience to earthquake-induced liquefaction disasters". Objectives and extent of ground characterization for Canakkale test site includes pre-existing soil investigation studies and complementary field studies. There were several SPT and geophysical tests carried out in the study area. Within the context of the complementary tests, six (6) study areas in the test site were chosen and complementary tests were carried out in these areas. In these areas, additional boreholes were opened and SPT tests were performed. It was decided that additional CPT (CPTU and SCPT) and Marchetti Dilatometer (DMT) tests should be carried out within the scope of the complementary testing. Seismic refraction, MASW and micro tremor measurements had been carried out in pre-existing studies. Shear wave velocities obtained from MASW measurements were evaluated to the most rigorous level. These tests were downhole seismic, PS-logging, seismic refraction, 2D-ReMi, MASW, micro tremor (H/V Nakamura method), 2D resistivity and resonance acoustic profiling (RAP). RAP is a new technique which will be explained briefly in the relevant section. Dynamic soil properties had not been measured in pre-existing studies, therefore these properties were investigated within the scope of the complementary tests. Selection of specific experimental tests of the complementary campaign was based on cost-benefit considerations Within the context of complementary field studies, dynamic soil properties were measured using resonant column and cyclic direct shear tests. Several sieve analyses and Atterberg Limits tests which were documented in the pre-existing studies were evaluated. In the complementary study carried out

  4. 77 FR 11112 - Proposed Approval of the Central Characterization Project's Remote-Handled Transuranic Waste...

    2012-02-24

    ... debris waste from the FB-Line at SRS. This waste was generated by glovebox operations, decontamination... summary category group solids (S3000) or soils and gravel (S4000) is characterized for WIPP disposal; and...

  5. Implementation of Agile project management in spent nuclear fuel characterization process

    Vinas Pena, P.

    2015-01-01

    Full text of publication follows. Spent nuclear fuel characterization (SNFC) is a complex process that covers different areas of analysis and whose final goal is to provide an accurate description of spent nuclear fuel (SNF) status for its future classification for storage and transport. The need to reduce the SNFC processing time maintaining the quality of the product has motivated ENUSA to research and implement Agile project management and human performance techniques. The Agile management techniques are focused in accommodate changes or new requirements in the project during the elaboration process without suffering delays or lose of quality. For its SNF projects ENUSA uses 2 complementary techniques: SCRUM and Kanban. SCRUM methodology is based on divide the process into activities blocks. Each block is a finished part of the final product which allows periodical deliveries of the product and the easy introduction of changes if they are necessary. The characterization process is formed by blocks of activities based on different analysis for every fuel assembly as the existence of leaking rods; the analysis of the structural integrity considering the existence of missing rods, broken or missing grids or grid straps or grid springs...; the corrosion phenomenon on the rod that could affect its integrity during the storage and transport; the burnup of the fuel assembly; the analysis of the rod internal pressure and its effect on rod failure mechanism as creep or on the material embrittlement due to the radial hydride precipitation; the compatibility with the container to avoid operational problems during cask loading and unloading, and any new input based on the regulatory evolution and the industry state of the art. The different analysis can be developed at the same time as they are independent. Kanban methodology consists in a visual representation of the evolution of the process. In a chart, the different activities needed to perform any of the analysis

  6. Characterization of physical and chemical properties of QLARIVIA-line of deuterium depleted Water

    Ferdes, Ov. S.; Mladin, C.; Petre, R.M.; Mitu, F.; Costinel, Diana; Vremera, Raluca; Sandru, Claudia

    2008-01-01

    QLARIVIA is the brand-name of Deuterium Depleted Water line of products of drinking water differentiating mainly by the deuterium concentration. It is the result of a national technological transfer project and it is based on an original, patented technology for deuterium depletion from the normal water. The paper presents the measuring and analysis results of the chemical and physical properties of the QLARIVIA brand-line of drinking DDW, as: pH; water hardness; permanganate index; Ca; Mg; Cl - ; SO4 2 - ; NH 4 ; NO 3 - ; NO 2 - , as well as the deuterium concentration determination by mass spectrometry. The analysis has been performed on at least 20 batch-samples, by usual, standardized and/or validated analytical methods, in ISO 17025:2005 accredited laboratories. The results are discussed considering the requirements of the EU directive on drinking water as well as of the Romanian Act on drinking water no. 458/2002 with its supplemental modification by the Act no.363/2004. The conclusion is that QLARIVIA - brand line of drinking DDW fulfills all the official physical and chemical requirements for the drinking water. (authors)

  7. Derivation and characterization of the NIH registry human stem cell line NYSCF100 line under defined feeder-free conditions

    Ana Sevilla

    2018-05-01

    Full Text Available The human embryonic stem cell line NYSCFe001-A was derived from a day 6 blastocyst in feeder-free and antibiotic free conditions. The blastocyst was voluntarily donated for research as surplus after in vitro fertilization treatment following informed consent. The NYSCFe001-A line, registered as NYSCF100 on the NIH registry, presents normal karyotype, is mycoplasma free, expresses all the pluripotency markers and has the potential to differentiate into all three germ layers in vitro.

  8. Generation and characterization of the first immortalized alpaca cell line suitable for diagnostic and immunization studies.

    Valentina Franceschi

    Full Text Available Raising of alpacas as exotic livestock for wool and meat production and as companion animals is growing in importance in the United States, Europe and Australia. Furthermore the alpaca, as well as the rest of the camelids, possesses the peculiarity of producing single-chain antibodies from which nanobodies can be generated. Nanobodies, due to their structural simplicity and reduced size, are very versatile in terms of manipulation and bio-therapeutic exploitation. In fact the biotech companies involved in nanobody production and application continue to grow in number and size. Hence, the development of reagents and tools to assist in the further growth of this new scientific and entrepreneurial reality is becoming a necessity. These are needed mainly to address alpaca disease diagnosis and prophylaxis, and to develop alpaca immunization strategies for nanobody generation. For instance an immortalized alpaca cell line would be extremely valuable. In the present work the first stabilized alpaca cell line from alpaca skin stromal cells (ASSCs was generated and characterized. This cell line was shown to be suitable for replication of viruses bovine herpesvirus-1, bovine viral diarrhea virus and caprine herpesvirus-1 and the endocellular parasite Neospora caninum. Moreover ASSCs were easy to transfect and transduce by several methods. These two latter characteristics are extremely useful when recombinant antigens need to be produced in a host homologous system. This work could be considered as a starting point for the expansion of the biotechnologies linked to alpaca farming and industry.

  9. Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala.

    Zhu, Dong-Mei; Yang, Kun; Wang, Wei-Min; Song, Wen

    2013-12-01

    This study established and characterized a new cell line (MAF) from the fin of blunt snout bream (Megalobrama amblycephala), a freshwater fish cultivated in China. MAF cells proliferated well in medium 199 supplemented with 10 % fetal bovine serum at 28 °C and have been subcultured more than 95 times in almost a year. MAF cells were revived at 90-95 % viability after 3-6 months of storage in liquid nitrogen. Karyotyping indicated that the modal chromosome number of MAF cells was 48. The MAF cell line consisted predominantly of fibroblastic and epithelial-like cells from M. amblycephala, which was confirmed by immunofluorescence and mitochondrial 12s rRNA sequencing. Viral susceptibility tests showed that MAF cells were susceptible to infection by snakehead rhabdovirus, spring viremia carp virus, and channel catfish virus, which was demonstrated by the presence of cytopathic effect, high viral titers, and PCR products. Bacterial cytotoxicity studies showed that extracellular products from Aeromonas hydrophila were toxic to MAF cells. Cu²⁺ was also cytotoxic to MAF cells, and the 24-h IC₅₀ value was 144.48 μmol/l. When MAF cells were transfected with pEGFP-N1 plasmid, bright fluorescent signals were observed, and the transfection efficiency reached up to 5 %. These results suggest that the MAF cell line may provide a valuable tool for studying virus pathogenesis, as well as cytotoxicity testing and genetic manipulation studies.

  10. Derivation and characterization of human embryonic stem cell lines from the Chinese population

    Zhao Wu; Huimin Dai; Lei Qian; Qing Tian; Lei Xiao; Xiaojun Tan; Hui Li; Lingjun Rao; Lixiazi He; Lei Bao; Jing Liao; Chun Cui; Zhenyu Zuo; Qiao Li

    2011-01-01

    Human embryonic stem cells (hESCs) can self-renew indefinitely and differentiate into all cell types in the human body. Therefore, they are valuable in regenerative medicine, human developmental biology and drug discovery. A number of hESC lines have been derived from the Chinese population,but limited of them are available for research purposes. Here we report the derivation and characterization of two hESC lines derived from human blastocysts of Chinese origin. These hESCs express alkaline phosphatase and hESC-specific markers, including Oct4, Nanog, SSEA-3, SSEA-4,TRA-1-60 and TRA-1-81. They also have high levels of telomerase activity and normal karyotypes. These cells can form embryoid body in vitro and can be differentiated into all three germ layers in vivo by teratoma formation. The newly established hESCs will be distributed for research purposes.The availability of hESC lines from the Chinese population will facilitate studies on the differences in hESCs from different ethnic groups.

  11. Temperature dependence of dynamical permeability characterization of magnetic thin films using shorted microstrip line probe

    Li, Xiling; Li, Chengyi; Chai, Guozhi

    2017-01-01

    A temperature dependence microwave permeability characterization system of magnetic thin film up to 10 GHz is designed and fabricated. This system can be used at temperatures ranging from room temperature to 200 °C, and is based on a shorted microstrip probe, which is made by microwave printed circuit board. Without contacting the magnetic thin films to the probe, the microwave permeability of the film can be detected without any limitations of sample size and with almost the same accuracy, as shown by comparison with the results obtained from a shorted microstrip transmission-line fixture. The complex permeability can be deduced by an analytical approach from the measured reflection coefficient of a strip line ( S 11 ) with and without a ferromagnetic film material on it. The procedures are the same with the shorted microstrip transmission-line method. The microwave permeability of an oblique deposited CoZr thin film was investigated with this probe. The results show that the room temperature dynamic permeability of the CoZr film is in good agreement with the results obtained from the established short-circuited microstrip perturbation method. The temperature dependence permeability results fit well with the Landau–Lifshitz–Gilbert equation. Development of the temperature-dependent measurement of the magnetic properties of magnetic thin film may be useful for the high-frequency application of magnetic devices at high temperatures. (paper)

  12. Advanced Research Projects Agency on Materials Preparation and Characterization Research

    Briefly summarized is research concerned with such topics as: Preparation of silica glass from amorphous silica; Glass structure by Raman ...ferroelectrics; Silver iodide crystals; Vapor phase growth; Refractory optical host materials; Hydroxyapatite ; Calcite; Characterization of single crystals with a double crystal spectrometer; Characterization of residual strain.

  13. Memorandum of understanding for the TWRS characterization retrieval project

    JO, J.

    1999-01-01

    During fiscal year 1999, various programs and projects will require analyses from the 222-S Laboratory. In order to prioritize and successfully meet the analytical demands of all the customers, multiple organizations across the Tank Waste Remediation System (TWRS) must work together. One of the projects that will require analyses from the 222-S Laboratory is the retrieval project. Successful completion of retrieval project objectives requires cooperation among four TWRS organizations. The requirements and expectations regarding retrieval project analytical work were examined in order to define roles and responsibilities for each of these TWRS organizations. This memorandum of understanding (MOU) documents the results of this review and establishes the roles and responsibilities. This MOU is subdivided into three sections, the first of which is this introduction. Section 2 provides a summary of the overall roles and responsibilities with respect to retrieval project analytical work for the four TWRS organizations involved. The final section describes the individual interfaces involved in performing retrieval project analytical work, and discusses the roles and responsibilities with respect to each individual interface

  14. Density-independent population projection trajectories of chromosome-substituted lines resistant and susceptible to organophosphate insecticides in Drosophila melanogaster

    Miyo Takahiro

    2004-11-01

    Full Text Available Abstract Background Seasonal fluctuations in susceptibility to organophosphate insecticides were observed in the Katsunuma population of Drosophila melanogaster for two consecutive years; susceptibility to three organophosphates tended to increase in the fall. To examine the hypothesis that variation in fitness among resistant and susceptible genotypes could trigger the change of genetic constitution within the fall population, we investigated density-independent population projection trajectories starting from single adult females with characteristics of chromosome-substituted lines resistant and susceptible to the three organophosphates. Results Density-independent population projection trajectories, expressed as the ratios of the number of each chromosome-substituted line to that of line SSS, for which all chromosomes were derived from the susceptible line, showed significant declines in numbers with time for all the resistant chromosome-substituted lines. Conclusion The declining tendency in the density-independent population projection trajectories of the resistant chromosome-substituted lines could explain the simultaneous decline in the levels of resistance to the three organophosphates, observed in the Katsunuma population in the fall.

  15. Big George to Carter Mountain 115-kV transmission line project, Park and Hot Springs Counties, Wyoming. Environmental Assessment

    1994-02-01

    The Western Area Power Administration (Western) is proposing to rebuild, operate, and maintain a 115-kilovolt (kV) transmission line between the Big George and Carter Mountain Substations in northwest Wyoming (Park and Hot Springs Counties). This environmental assessment (EA) was prepared in compliance with the National Environmental Policy Act (NEPA) and the regulations of the Council on Environmental Quality (CEQ) and the Department of Energy (DOE). The existing Big George to Carter Mountain 69-kV transmission line was constructed in 1941 by the US Department of Interior, Bureau of Reclamation, with 1/0 copper conductor on wood-pole H-frame structures without an overhead ground wire. The line should be replaced because of the deteriorated condition of the wood-pole H-frame structures. Because the line lacks an overhead ground wire, it is subject to numerous outages caused by lightning. The line will be 54 years old in 1995, which is the target date for line replacement. The normal service life of a wood-pole line is 45 years. Under the No Action Alternative, no new transmission lines would be built in the project area. The existing 69-kV transmission line would continue to operate with routine maintenance, with no provisions made for replacement.

  16. Beyond the line: exploring the HRM responsibilities of line managers, project managers and the HRM department in four project-oriented companies in the Netherlands, Austria, the UK and the USA

    Keegan, A.; Huemann, M.; Turner, J.R.

    2012-01-01

    The topic of what human resource management (HRM) responsibilities are devolved from the HRM department to line managers has attracted much interest in recent years. We report findings from a study on the devolution of HRM practices in four project-oriented companies (POCs) and argue that although

  17. 76 FR 16609 - Proposed Information Collection; Comment Request; Identification of Human Cell Lines Project

    2011-03-24

    ... differentiate among cell lines, as described in Designation: ASN-0002 Authentication of Human Cell Lines... NIST (contact information above). III. Data OMB Control Number: None. Form Number: None. Type of Review...

  18. Characterization and optimization of the R A-6 s on-line neutron radiography facility

    Mezio Guanes, Federico Andres

    2007-01-01

    With the objective of characterizing and optimizing the radiation-field filters behavior in the beam of the R A-6 on-line Neutron Radiography facility, some improvements have been done to the facility devices.We studied the camera sensibility, the best camera acquisition software configuration, the best depth of field, we increased the system tuning efficiency.We also studied the linearity of the facility vs the reactor core neutron fluence and finally we constructed a device that ensure the repeatability of the measurements.The main parameters chosen to represent the best radiation-field set-up are the thermal neutron flux and dose in the position of the camera.Finally, a camera shield optimization haven been done in function of its position [es

  19. Characterization of a continuous feline mammary epithelial cell line susceptible to feline epitheliotropic viruses.

    Pesavento, Patricia; Liu, Hongwei; Ossiboff, Robert J; Stucker, Karla M; Heymer, Anna; Millon, Lee; Wood, Jason; van der List, Deborah; Parker, John S L

    2009-04-01

    Mucosal epithelial cells are the primary targets for many common viral pathogens of cats. Viral infection of epithelia can damage or disrupt the epithelial barrier that protects underlying tissues. In vitro cell culture systems are an effective means to study how viruses infect and disrupt epithelial barriers, however no true continuous or immortalized feline epithelial cell culture lines are available. A continuous cell culture of feline mammary epithelial cells (FMEC UCD-04-2) that forms tight junctions with high transepithelial electrical resistance (>2000Omegacm(-1)) 3-4 days after reaching confluence was characterized. In addition, it was shown that FMECs are susceptible to infection with feline calicivirus (FCV), feline herpesvirus (FHV-1), feline coronavirus (FeCoV), and feline panleukopenia virus (FPV). These cells will be useful for studies of feline viral disease and for in vitro studies of feline epithelia.

  20. Uncertainty characterization of particle depth measurement using digital in-line holography and the hybrid method.

    Gao, Jian; Guildenbecher, Daniel R; Reu, Phillip L; Chen, Jun

    2013-11-04

    In the detection of particles using digital in-line holography, measurement accuracy is substantially influenced by the hologram processing method. In particular, a number of methods have been proposed to determine the out-of-plane particle depth (z location). However, due to the lack of consistent uncertainty characterization, it has been unclear which method is best suited to a given measurement problem. In this work, depth determination accuracies of seven particle detection methods, including a recently proposed hybrid method, are systematically investigated in terms of relative depth measurement errors and uncertainties. Both synthetic and experimental holograms of particle fields are considered at conditions relevant to particle sizing and tracking. While all methods display a range of particle conditions where they are most accurate, in general the hybrid method is shown to be the most robust with depth uncertainty less than twice the particle diameter over a wide range of particle field conditions.

  1. Yucca Mountain Site Characterization Project bibliography, January--June 1995. Supplement 4, Add.3: An update

    Stephan, P.M. [ed.

    1996-01-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy`s Energy Science and Technology Database from January 1, 1995, through June 30, 1995. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers, and articles are included in the sponsoring organization`s list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it.

  2. Yucca Mountain Site Characterization Project bibliography, July--December 1992: An update, Supplement 3, Addendum 2

    1993-04-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy's Energy Science and Technology Database from July 1, 1992, through December 31, 1992. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers, and articles are included in the sponsoring organization's list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it

  3. Yucca Mountain Site Characterization Project Bibliography, January--June 1993. An update: Supplement 4, Addendum 1

    Stephan, P.M. [ed.

    1995-01-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy`s Energy Science and Technology Database from January 1, 1994 through June 30, 1994. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers,and articles are included in the sponsoring organization`s list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it.

  4. Yucca Mountain Site Characterization Project Bibliography, January--June 1993. An update: Supplement 4, Addendum 1

    Stephan, P.M.

    1995-01-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy's Energy Science and Technology Database from January 1, 1994 through June 30, 1994. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers,and articles are included in the sponsoring organization's list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it

  5. Yucca Mountain Site Characterization Project bibliography, January--June 1995. Supplement 4, Add.3: An update

    Stephan, P.M.

    1996-01-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy's Energy Science and Technology Database from January 1, 1995, through June 30, 1995. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers, and articles are included in the sponsoring organization's list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it

  6. TWRS privatization support project waste characterization resource dictionary

    Patello, G.K.; Wiemers, K.D.

    1996-09-01

    A single estimate of waste characteristics for each underground storage tanks at the Hanford Site is not available. The information that is available was developed for specific programmatic objectives and varies in format and level of descriptive detail, depending on the intended application. This dictionary reflects an attempt to define what waste characterization information is available. It shows the relationship between the identified resource and the original data source and the inter-relationships among the resources; it also provides a brief description of each resource. Developed as a general dictionary for waste characterization information, this document is intended to make the user aware of potenially useful resources

  7. Nahm transformation for parabolic Higgs bundles on the projective line-Case of non-semisimple residues

    Szabó, Szilárd

    2017-12-01

    We extend our earlier construction of Nahm transformation for parabolic Higgs bundles on the projective line to solutions with not necessarily semisimple residues and show that it determines a holomorphic mapping on corresponding moduli spaces. The construction relies on suitable elementary modifications of the logarithmic Dolbeault complex.

  8. Isolation and characterization of a spontaneously immortalized bovine retinal pigmented epithelial cell line

    Griffiths T Daniel

    2009-05-01

    Full Text Available Abstract Background The Retinal Pigmented Epithelium (RPE is juxtaposed with the photoreceptor outer segments of the eye. The proximity of the photoreceptor cells is a prerequisite for their survival, as they depend on the RPE to remove the outer segments and are also influenced by RPE cell paracrine factors. RPE cell death can cause a progressive loss of photoreceptor function, which can diminish vision and, over time, blindness ensues. Degeneration of the retina has been shown to induce a variety of retinopathies, such as Stargardt's disease, Cone-Rod Dystrophy (CRD, Retinitis Pigmentosa (RP, Fundus Flavimaculatus (FFM, Best's disease and Age-related Macular Degeneration (AMD. We have cultured primary bovine RPE cells to gain a further understanding of the mechanisms of RPE cell death. One of the cultures, named tRPE, surpassed senescence and was further characterized to determine its viability as a model for retinal diseases. Results The tRPE cell line has been passaged up to 150 population doublings and was shown to be morphologically similar to primary cells. They have been characterized to be of RPE origin by reverse transcriptase PCR and immunocytochemistry using the RPE-specific genes RPE65 and CRALBP and RPE-specific proteins RPE65 and Bestrophin. The tRPE cells are also immunoreactive to vimentin, cytokeratin and zonula occludens-1 antibodies. Chromosome analysis indicates a normal diploid number. The tRPE cells do not grow in suspension or in soft agar. After 3H thymidine incorporation, the cells do not appear to divide appreciably after confluency. Conclusion The tRPE cells are immortal, but still exhibit contact inhibition, serum dependence, monolayer growth and secrete an extra-cellular matrix. They retain the in-vivo morphology, gene expression and cell polarity. Additionally, the cells endocytose exogenous melanin, A2E and purified lipofuscin granules. This cell line may be a useful in-vitro research model for retinal

  9. Improved cell line IPEC-J2, characterized as a model for porcine jejunal epithelium.

    Silke S Zakrzewski

    Full Text Available Cell lines matching the source epithelium are indispensable for investigating porcine intestinal transport and barrier properties on a subcellular or molecular level and furthermore help to reduce animal usage. The porcine jejunal cell line IPEC-J2 is established as an in vitro model for porcine infection studies but exhibits atypically high transepithelial resistances (TER and only low active transport rates so that the effect of nutritional factors cannot be reliably investigated. This study aimed to properly remodel IPEC-J2 and then to re-characterize these cells regarding epithelial architecture, expression of barrier-relevant tight junction (TJ proteins, adequate TER and transport function, and reaction to secretagogues. For this, IPEC-J2 monolayers were cultured on permeable supports, either under conventional (fetal bovine serum, FBS or species-specific (porcine serum, PS conditions. Porcine jejunal mucosa was analyzed for comparison. Main results were that under PS conditions (IPEC-J2/PS, compared to conventional FBS culture (IPEC-J2/FBS, the cell height increased 6-fold while the cell diameter was reduced by 50%. The apical cell membrane of IPEC-J2/PS exhibited typical microvilli. Most importantly, PS caused a one order of magnitude reduction of TER and of trans- and paracellular resistance, and a 2-fold increase in secretory response to forskolin when compared to FBS condition. TJ ultrastructure and appearance of TJ proteins changed dramatically in IPEC-J2/PS. Most parameters measured under PS conditions were much closer to those of typical pig jejunocytes than ever reported since the cell line's initial establishment in 1989. In conclusion, IPEC-J2, if cultured under defined species-specific conditions, forms a suitable model for investigating porcine paracellular intestinal barrier function.

  10. Plasma density characterization at SPARC-LAB through Stark broadening of Hydrogen spectral lines

    Filippi, F.; Anania, M.P.; Bellaveglia, M.; Biagioni, A.; Chiadroni, E.; Cianchi, A.; Di Giovenale, D.; Di Pirro, G.; Ferrario, M.; Mostacci, A.; Palumbo, L.; Pompili, R.; Shpakov, V.; Vaccarezza, C.; Villa, F.; Zigler, A.

    2016-01-01

    Plasma-based acceleration techniques are of great interest for future, compact accelerators due to their high accelerating gradient. Both particle-driven and laser-driven Plasma Wakefield Acceleration experiments are foreseen at the SPARC-LAB Test Facility (INFN National Laboratories of Frascati, Italy), with the aim to accelerate high-brightness electron beams. In order to optimize the efficiency of the acceleration in the plasma and preserve the quality of the accelerated beam, the knowledge of the plasma electron density is mandatory. The Stark broadening of the Hydrogen spectral lines is one of the candidates used to characterize plasma density. The implementation of this diagnostic for plasma-based experiments at SPARC-LAB is presented. - Highlights: • Stark broadening of Hydrogen lines has been measured to determine plasma density. • Plasma density diagnostic tool for plasma-based experiments at SPARC-LAB is presented. • Plasma density in tapered laser triggered ablative capillary discharge was measured. • Results of plasma density measurements in ablative capillaries are shown.

  11. Plasma density characterization at SPARC-LAB through Stark broadening of Hydrogen spectral lines

    Filippi, F., E-mail: francesco.filippi@roma1.infn.it [Dipartimento di Scienze di Base e Applicate per l' Ingegneria (SBAI), ‘Sapienza’ Università di Roma, Via A. Scarpa 14-16, 00161 Roma (Italy); INFN-Roma1, Piazzale Aldo Moro, 2 00161 Roma (Italy); Anania, M.P.; Bellaveglia, M.; Biagioni, A.; Chiadroni, E. [Laboratori Nazionali di Frascati, INFN, Via E. Fermi, Frascati (Italy); Cianchi, A. [Dipartimento di Fisica, Universitá di Roma Tor Vergata, Via della Ricerca Scientifica 1, 00133 Roma (Italy); Di Giovenale, D.; Di Pirro, G.; Ferrario, M. [Laboratori Nazionali di Frascati, INFN, Via E. Fermi, Frascati (Italy); Mostacci, A.; Palumbo, L. [Dipartimento di Scienze di Base e Applicate per l' Ingegneria (SBAI), ‘Sapienza’ Università di Roma, Via A. Scarpa 14-16, 00161 Roma (Italy); INFN-Roma1, Piazzale Aldo Moro, 2 00161 Roma (Italy); Pompili, R.; Shpakov, V.; Vaccarezza, C.; Villa, F. [Laboratori Nazionali di Frascati, INFN, Via E. Fermi, Frascati (Italy); Zigler, A. [Hebrew University of Jerusalem, Jerusalem 91904 (Israel)

    2016-09-01

    Plasma-based acceleration techniques are of great interest for future, compact accelerators due to their high accelerating gradient. Both particle-driven and laser-driven Plasma Wakefield Acceleration experiments are foreseen at the SPARC-LAB Test Facility (INFN National Laboratories of Frascati, Italy), with the aim to accelerate high-brightness electron beams. In order to optimize the efficiency of the acceleration in the plasma and preserve the quality of the accelerated beam, the knowledge of the plasma electron density is mandatory. The Stark broadening of the Hydrogen spectral lines is one of the candidates used to characterize plasma density. The implementation of this diagnostic for plasma-based experiments at SPARC-LAB is presented. - Highlights: • Stark broadening of Hydrogen lines has been measured to determine plasma density. • Plasma density diagnostic tool for plasma-based experiments at SPARC-LAB is presented. • Plasma density in tapered laser triggered ablative capillary discharge was measured. • Results of plasma density measurements in ablative capillaries are shown.

  12. Experimental characterization of the flux-line lattice in superconducting V3Si

    Christen, D.; Chang, Y.; Kerchner, H.; Larson, B.; Narayan, J.; SeKula, S.

    1983-01-01

    Several microscopic properties of the flux-line lattice (FLL) in three separate single crystals of V 3 Si have been investigated by means of small-angle neutron diffraction. These low-field FLL characterizations have been correlated with the following material and superconducting properties: the real crystal symmetry parallel to the applied magnetic field; the micro-structure as determined by TEM; magnetic irreversibilities in the mixed state; reversible flux-line motion in ac response; martensitic structural transformation observed by X-ray diffraction. The three samples, V 3 Si-MP3, -MP4, and -MP5 possessed different defect structures, and this was manifested foremost in the FLL perfection. At low field (B 0-.5 T, but a highly mosaic, nearly polycrystalline FLL at lower fields. Sample MP4 contained large (500-1000 A) incoherent precipitates, and showed only a polycrystalline FLL at low field. In both MP3 and MP5, distinct anisotropic correlations were observed between the FLL morphology and the real-crystal direction along the applied field. The FLL perfection was strongly dependent on the growth history. The peak width history dependence for two different scattering geometries can be qualitatively modeled by proposed flux-pinning mechanisms. Quantitative comparisons with critical current measurements, however, are not totally reconcilable

  13. Proposed particle-beam characterizations for the APS undulator test line

    Lumpkin, A.H.; Borland, M.; Milton, S.

    1993-09-01

    A research and development effort is underway at the Advanced Photon Source (APS) to use an rf gun as a low-emittance electron source for injection into the 100- to 650-MeV linac subsystem and subsequently to an undulator test area. This configuration would combine the acceleration capability of the 200-MeV S-band electron linac and the in-line 450-MeV positron linac that normally provide positrons to the positron accumulator ring (PAR). A transport line that bypasses the PAR will bring the electrons to the undulator test area. Characterization techniques will be discussed for the electron beam with a normalized, rms emittance of <10 {pi} mm mrad (1{sigma}) at micropulse charges of up to 350 pC and micropulse durations of {approximately}5 ps (FWHM). Tests proposed include measurement of particle beam transport effects (at one-tenth the storage ring beam rigidity) caused by small undulator field errors as well as operations intended to produce coherent, short wavelength radiation (<200 nm).

  14. Cytokeratin characterization of human prostatic carcinoma and its derived cell lines.

    Nagle, R B; Ahmann, F R; McDaniel, K M; Paquin, M L; Clark, V A; Celniker, A

    1987-01-01

    Two murine monoclonal anti-cytokeratin antibodies with defined specificity were shown to distinguish between basal cells and luminal cells in human prostate tissue. Forty-one biopsies or transurethral resection specimens were characterized using these two antibodies. In cases of benign prostatic hyperplasia, focal loss of the basal cell layer was noted in areas of glandular proliferation. Ten cases of adenocarcinoma of the prostate, varying in Gleason's histological grade from 2 to 4, were also studied. In each case the carcinoma was shown to represent the luminal cell phenotype with no evidence of involvement of the basal cell phenotype. An analysis of three established metastatic prostatic carcinoma cell lines (DU-145, PC-3, and LNCaP) using two-dimensional electrophoresis showed that the cytokeratin complement of each cell line was slightly different but retained the phenotype of the luminal cell. It was concluded that during both hyperplasia and neoplastic transformation of the prostate, the luminal cell phenotype is primarily involved and that the basal cell phenotype does not appear to contribute to either intraluminal proliferation or invasive cell populations.

  15. Re-animated heritage. National project Nieuwe Hollandse Waterlinie (New Dutch Water Defence Line as a format for landscape policy

    Eric Luiten

    2011-12-01

    Full Text Available Landscape policy has always been characterized, often supported but just as often hindered by its multiple origins in both landscape protection and landscape development. Along the recalcitrant and frequently inconsistent lines of preservation of monuments, nature conservation, land use, open-air recreation and the planting of new woods and shrubs respectively, landscape protection did not develop without difficulty. On a government level landscape protection was most extensively laid down in the Structure Plan for Nature Conservation and Protection of the Countryside. In this Plan the government introduced an extensive series of landscape categories, among which National Parks, National Landscapes, Valuable Agricultural Cultural Landscapes, Natural Heritage Valuable Areas, Large Landscape Unities and Valuable Scenic and/or Historical Views. In the Green Space Structure Plan from 1994 the differentiation of landscape policy was drastically reduced to the category of Valuable Cultural Landscapes and in addition a reference was made to the intended execution of large projects, such as the Randstad Green Structure and the National Ecological Network. In the revision of this Structural Plan in 2002 the category of National Landscapes was reintroduced. From the quarter of landscape development approximately every ten years a policy document is issued. The View on Landscape Creation (dating from the seventies, the View on Landscape (eighties and the Policy Document on Landscape (nineties are to be regarded as attempts at a synthesis of the various motives behind landscape development. The gist of these policy documents shifts from manifests for national green areas towards worked-out, integral concepts and formulas for rural-area development and closes with more and more complicatedly formulated recommendations, such as in the Development-oriented Landscape Strategy. After the Policy Document on Landscape with poorly argued maps of the so

  16. DQO Summary Report for 105-N/109-N Interim Safe Storage Project Waste Characterization

    T. A. Lee

    2005-09-15

    The DQO summary report provides the results of the DQO process completed for waste characterization activities for the 105-N/109-N Reactor Interim Safe Storage Project including decommission, deactivate, decontaminate, and demolish activities for six associated buildings.

  17. DQO Summary Report for 105-N/109-N Interim Safe Storage Project Waste Characterization

    Lee, T.A.

    2005-01-01

    The DQO summary report provides the results of the DQO process completed for waste characterization activities for the 105-N/109-N Reactor Interim Safe Storage Project including decommission, deactivate, decontaminate, and demolish activities for six associated buildings.

  18. THE PHOTOMETRIC AND KINEMATIC STRUCTURE OF FACE-ON DISK GALAXIES. II. INTEGRATED LINE PROFILE CHARACTERIZATION AND THE ORIGIN OF LINE PROFILE ASYMMETRY

    Andersen, David R.; Bershady, Matthew A.

    2009-01-01

    We perform a moments analysis of H I and H II global line profiles for 33 nearly face-on disk galaxies for the threefold purpose of rationalizing and interpreting line profile indices in the literature, presenting robust moment definitions with analytic error functions calibrated against Monte Carlo simulation, and probing the physical origin of line profile asymmetries. The first four profile moments serve as viable surrogates for the recession velocity, line width, asymmetry, and profile shape, respectively. The first three moments are superior, by a factor of ∼2 in precision, to related quantities defined in the literature. First and third profile moments are related; skew can be used to calculate more accurate recession velocities from global profiles. Second and fourth profile moments are linked, corresponding to the known trend that narrow (but well resolved) line widths tend to be more Gaussian. Hα kurtosis also appears correlated with the spatially resolved line width of the ionized gas. No systematics appear between various measures of line width and true rotation speed other than the wide range of normalizations, which we calibrate. This conclusion and calibration, however, is highly sample dependent. The ratio of H II to H I widths is consistent with unity, even at low projected line width. There may be a trend toward a decrease in the ratio of H II to H I widths consistent with previous studies, but we also observe greater scatter. While there is good agreement between H I and H II first, second, and fourth moments, we find no positive correlation between skew measured from H I and H II profiles. Detailed analysis of the spatially resolved Hα distribution demonstrates that H II global profile asymmetries are dominated by differences in the gas distribution, not kinematic asymmetries.

  19. The Genomes OnLine Database (GOLD) v.4: status of genomic and metagenomic projects and their associated metadata

    Pagani, Ioanna; Liolios, Konstantinos; Jansson, Jakob; Chen, I-Min A.; Smirnova, Tatyana; Nosrat, Bahador; Markowitz, Victor M.; Kyrpides, Nikos C.

    2012-01-01

    The Genomes OnLine Database (GOLD, http://www.genomesonline.org/) is a comprehensive resource for centralized monitoring of genome and metagenome projects worldwide. Both complete and ongoing projects, along with their associated metadata, can be accessed in GOLD through precomputed tables and a search page. As of September 2011, GOLD, now on version 4.0, contains information for 11 472 sequencing projects, of which 2907 have been completed and their sequence data has been deposited in a public repository. Out of these complete projects, 1918 are finished and 989 are permanent drafts. Moreover, GOLD contains information for 340 metagenome studies associated with 1927 metagenome samples. GOLD continues to expand, moving toward the goal of providing the most comprehensive repository of metadata information related to the projects and their organisms/environments in accordance with the Minimum Information about any (x) Sequence specification and beyond. PMID:22135293

  20. Metabolic characterization of invaded cells of the pancreatic cancer cell line, PANC-1.

    Fujita, Mayumi; Imadome, Kaori; Imai, Takashi

    2017-05-01

    We previously reported that about 0.4% of cells in the cultured human pancreatic cancer cell line, PANC-1, can invade matrigel during the transwell invasion assay, suggesting that these invaded PANC-1 cells may have specific characteristics to keep their invasive potential. To identify the metabolic characterization specific in the invaded PANC-1 cells, metabolome analysis of the invaded PANC-1 compared with the whole cultured PANC-1 was performed using CE-TOFMS, and concentrations of 110 metabolites were measured. In contrast to the whole cultured cells, the invaded PANC-1 was characterized as a population with reduced levels of amino acids and TCA cycle intermediates, and decreased and increased intermediates in glycolysis and nucleic acid metabolism. In particular, the ratio of both adenosine and guanosine energy charge was reduced in the invaded cells, revealing that the consumption of ATP and GTP was high in the invaded cells, and thus suggesting that ATP- or GTP-generating pathways are stimulated. In addition, the GSH/GSSG ratio was low in the invaded cells, but these cells had a higher surviving fraction after exposure to hydrogen peroxide. Thus, the invaded cells were the population resistant to oxidative stress. Furthermore, reduction in intracellular GSH content inhibited PANC-1 invasiveness, indicated that GSH has an important role in PANC-1 invasiveness. Overall, we propose the invaded cells have several unique metabolic profiles. © 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

  1. In-line characterization and identification of micro-droplets on-chip

    Weber Emanuel

    2014-01-01

    Full Text Available We present an integrated optofluidic sensor system for in-line characterization of micro-droplets. The device provides information about the droplet generation frequency, the droplet volume, and the content of the droplet. Due to its simplicity this principle can easily be implemented with other microfluidic components on one and the same device. The sensor is based on total internal reflection phenomena. Droplets are pushed through a microfluidic channel which is hit by slightly diverging monochromatic light. At the solid-liquid interface parts of the rays experience total internal reflection while another part is transmitted. The ratio of reflected to transmitted light depends on the refractive index of the solution. Both signals are recorded simultaneously and provide a very stable output signal for the droplet characterization. With the proposed system passing droplets were counted up to 320 droplets per second and droplets with different volumes could be discriminated. In a final experiment droplets with different amounts of dissolved CaCl2 were distinguished based on their reflected and transmitted light pattern. This principle can be applied for the detection of any molecules in microdroplets which significantly influence the refractive index of the buffer solution.

  2. Use of GEANT4 vs. MCNPX for the characterization of a boron-lined neutron detector

    Ende, B.M. van der; Atanackovic, J.; Erlandson, A.; Bentoumi, G.

    2016-06-01

    This work compares GEANT4 with MCNPX in the characterization of a boron-lined neutron detector. The neutron energy ranges simulated in this work (0.025 eV to 20 MeV) are the traditional domain of MCNP simulations. This paper addresses the question, how well can GEANT4 and MCNPX be employed for detailed thermal neutron detector characterization? To answer this, GEANT4 and MCNPX have been employed to simulate detector response to a {sup 252}Cf energy spectrum point source, as well as to simulate mono-energetic parallel beam source geometries. The {sup 252}Cf energy spectrum simulation results demonstrate agreement in detector count rate within 3% between the two packages, with the MCNPX results being generally closer to experiment than are those from GEANT4. The mono-energetic source simulations demonstrate agreement in detector response within 5% between the two packages for all neutron energies, and within 1% for neutron energies between 100 eV and 5 MeV. Cross-checks between the two types of simulations using ISO-8529 {sup 252}Cf energy bins demonstrates that MCNPX results are more self-consistent than are GEANT4 results, by 3–4%.

  3. Characterization of pipes, drain lines, and ducts using the pipe explorer system

    Cremer, C.D.; Kendrick, D.T.; Cramer, E.

    1997-01-01

    As DOE dismantles its nuclear processing facilities, site managers must employ the best means of disposing or remediating hundreds of miles of potentially contaminated piping and duct work. Their interiors are difficult to access, and in many cases even the exteriors are inaccessible. Without adequate characterization, it must be assumed that the piping is contaminated, and the disposal cost of buried drain lines can be on the order of $1,200/ft and is often unnecessary as residual contamination levels often are below free release criteria. This paper describes the program to develop a solution to the problem of characterizing radioactive contamination in pipes. The technical approach and results of using the Pipe Explorer trademark system are presented. The heart of the system is SEA's pressurized inverting membrane adapted to transport radiation detectors and other tools into pipes. It offers many benefits over other pipe inspection approaches. It has video and beta/gamma detection capabilities, and the need for alpha detection has been addressed through the development of the Alpha Explorer trademark. These systems have been used during various stages of decontamination and decommissioning of DOE sites, including the ANL CP-5 reactor D ampersand D. Future improvements and extensions of their capabilities are discussed

  4. THz transceiver characterization : LDRD project 139363 final report.

    Nordquist, Christopher Daniel; Wanke, Michael Clement; Cich, Michael Joseph; Reno, John Louis; Fuller, Charles T.; Wendt, Joel Robert; Lee, Mark; Grine, Albert D.

    2009-09-01

    LDRD Project 139363 supported experiments to quantify the performance characteristics of monolithically integrated Schottky diode + quantum cascade laser (QCL) heterodyne mixers at terahertz (THz) frequencies. These integrated mixers are the first all-semiconductor THz devices to successfully incorporate a rectifying diode directly into the optical waveguide of a QCL, obviating the conventional optical coupling between a THz local oscillator and rectifier in a heterodyne mixer system. This integrated mixer was shown to function as a true heterodyne receiver of an externally received THz signal, a breakthrough which may lead to more widespread acceptance of this new THz technology paradigm. In addition, questions about QCL mode shifting in response to temperature, bias, and external feedback, and to what extent internal frequency locking can improve stability have been answered under this project.

  5. Establishment and characterization of a novel head and neck squamous cell carcinoma cell line USC-HN1.

    Liebertz, Daniel J; Lechner, Melissa G; Masood, Rizwan; Sinha, Uttam K; Han, Jing; Puri, Raj K; Correa, Adrian J; Epstein, Alan L

    2010-02-22

    Head and neck squamous cell carcinoma (HNSCC) is an aggressive and lethal malignancy. Publically available cell lines are mostly of lingual origin, or have not been carefully characterized. Detailed characterization of novel HNSCC cell lines is needed in order to provide researchers a concrete keystone on which to build their investigations. The USC-HN1 cell line was established from a primary maxillary HNSCC biopsy explant in tissue culture. The immortalized cells were then further characterized by heterotransplantation in Nude mice; immunohistochemical staining for relevant HNSCC biomarkers; flow cytometry for surface markers; cytogenetic karyotypic analysis; human papillomavirus and Epstein-Barr virus screening; qRT-PCR for oncogene and cytokine analysis; investigation of activated, cleaved Notch1 levels; and detailed 35,000 gene microarray analysis. Characterization experiments confirmed the human HNSCC origin of USC-HN1, including a phenotype similar to the original tumor. Viral screening revealed no HPV or EBV infection, while western blotting displayed significant upregulation of activated, cleaved Notch1. USC-HN1, a novel immortalized cell line has been derived from a maxillary HNSCC. Characterization studies have shown that the cell line is of HNSCC origin and displays many of the same markers previously reported in the literature. USC-HN1 is available for public research and will further the investigation of HNSCC and the development of new therapeutic modalities.

  6. Evaluation and Characterization of In-Line Annealed Continuous Cast Aluminum Sheet

    Dr Subodh K. Das

    2006-01-17

    significant energy, environmental and economic benefits thus benefiting a broad cross-section of the aluminum industry. The results from this project will have a positive impact on improving efficiencies in other areas of aluminum processing including Casting and in the Finished Products Sector of the roadmap Continuous casting (CC) leads to 25% energy savings in the production of aluminum sheet products compared to conventional DC casting as it does away with the intermediate steps of DC cast ingots and homogenization of the ingots and substituting it with a continuous casting process which goes directly into the hot rolling mill. The results of the proposed research will enable further applications of continuous casting technology. The aim here is to develop a continuous in line anneal process that will replace the existing batch type techniques thereby resulting in a continuous uni-directional movement of material from cast to final product. The goal of this multi-partner R&D project is to develop energy-efficient and optimized thermomechanical processing for the production of continuous cast 5000 series aluminum sheet alloys. Full scale implementation of the proposed effort by the year 2015 could lead to yearly energy savings of 23 trillion Btu and related energy cost savings of $96 million per year for the U. S. aluminum rolled products industry. The major achievements of this project based on the goals and objectives of the project are given.

  7. Qinshan phase II extension nuclear power project thermal stratification and fatigue stress analysis for pressurizer surge line

    Yu Xiaofei; Zhang Yixiong; Ai Honglei

    2010-01-01

    Thermal stratification of pressurizer surge line induced by the inside fluid brings on global bending moments, local thermal stresses, unexpected displacements and support loadings of the pipe system. In order to avoid a costly three-dimensional computation, a combined 1D/2D technique has been developed and implemented to analyze the thermal stratification and fatigue stress of pressurize surge line of QINSHAN Phase II Extension Nuclear Power Project in this paper, using the computer codes SYSTUS and ROCOCO. According to the mechanical analysis results of stratification, the maximum stress and cumulative usage factor, the loadings at connections of surge line to main pipe and RCP and the displacements of surge line at supports are obtained. (authors)

  8. Environmental assessment and finding of no significant impact: Hungry Horse--Columbia Falls line rebuild and relocation project

    1993-09-01

    Bonneville Power Administration (BPA) has identified a need to rebuild the Hungry Horse-Columbia Falls 115,000-volt (115-kV) transmission line to 230-kV. This line supplies power to customers in the area of Columbia Falls, Montana, and integrates generation at the US Bureau of Reclamation's (USBR) Hungry Horse Dam into BPA's transmission grid. There are several problems with the existing system. The Hungry Horse-Columbia Falls 1 15-kV line is 45 years old and requires excessive maintenance. The USBR has decided to replace their aging 115-kV transformers at the dam with 230-kV transformers, which also would increase their operational flexibility. With the small conductor size and voltage of the line presently being used, significant amounts of energy are lost as the power moves across the line. Transformer failure at Hungry Horse Dam has led to joint planning between BPA and the USBR. (USBR and the US Forest Service are cooperating agencies on the proposed project.) The proposal to eliminate the 115-kV equipment and convert to 230-kV operation was the least costly of the options studied. By rebuilding the line, maintenance costs (and time required for outages) would be reduced. The increased generation at the USBR dam would be safely and consistently transmitted over the improved system, and less energy would be lost from the line, a cost and energy savings

  9. Waste Tank Vapor Characterization Project: Annual status report for FY 1995

    Ligotke, M.W.; Fruchter, J.S.; Huckaby, J.L.; Birn, M.B.; McVeety, B.D.; Evans, J.C. Jr.; Pool, K.H.; Silvers, K.L.; Goheen, S.C.

    1995-11-01

    This report compiles information collected during the Fiscal Year 1995 pertaining to the waste tank vapor characterization project. Information covers the following topics: project management; organic sampling and analysis; inorganic sampling and analysis; waste tank vapor data reports; and the waste tanks vapor database

  10. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1995 quality program status report

    Bolivar, S.L.

    1996-07-01

    This status report summarizes the activities and accomplishments of the Los Alamos National Laboratory Yucca Mountain Site Characterization Project`s (YMP`s) quality assurance program for January 1 to September 30, 1995. The report includes major sections on program activities and trend analysis.

  11. Development of a Methodology for Hydrogeological Characterization of Faults: Progress of the Project in Berkeley, California

    Goto, J.; Moriya, T.; Yoshimura, K.; Tsuchi, H.; Karasaki, K.; Onishi, T.; Ueta, K.; Tanaka, S.; Kiho, K.

    2010-12-01

    The Nuclear Waste Management Organization of Japan (NUMO), in collaboration with Lawrence Berkeley National Laboratory (LBNL), has carried out a project to develop an efficient and practical methodology to characterize hydrologic property of faults since 2007, exclusively for the early stage of siting a deep underground repository. A preliminary flowchart of the characterization program and a classification scheme of fault hydrology based on the geological feature have been proposed. These have been tested through the field characterization program on the Wildcat Fault in Berkeley, California. The Wildcat Fault is a relatively large non-active strike-slip fault which is believed to be a subsidiary of the active Hayward Fault. Our classification scheme assumes the contrasting hydrologic features between the linear northern part and the split/spread southern part of the Wildcat Fault. The field characterization program to date has been concentrated in and around the LBNL site on the southern part of the fault. Several lines of electrical and reflection seismic surveys, and subsequent trench investigations, have revealed the approximate distribution and near-surface features of the Wildcat Fault (see also Onishi, et al. and Ueta, et al.). Three 150m deep boreholes, WF-1 to WF-3, have been drilled on a line normal to the trace of the fault in the LBNL site. Two vertical holes were placed to characterize the undisturbed Miocene sedimentary formations at the eastern and western sides of the fault (WF-1 and WF-2 respectively). WF-2 on the western side intersected the rock formation, which was expected only in WF-1, and several of various intensities. Therefore, WF-3, originally planned as inclined to penetrate the fault, was replaced by the vertical hole further to the west. It again encountered unexpected rocks and faults. Preliminary results of in-situ hydraulic tests suggested that the transmissivity of WF-1 is ten to one hundred times higher than WF-2. The monitoring

  12. Summer Student Project: GEM Simulation and Gas Mixture Characterization

    Oviedo Perhavec, Juan Felipe

    2013-01-01

    Abstract This project is a numerical simulation approach to Gas Electron Multiplier (GEM) detectors design. GEMs are a type of gaseous ionization detector that have proposed as an upgrade for CMS muon endcap. The main advantages of this technology are high spatial and time resolution and outstanding aging resistance. In this context, fundamental physical behavior of a Gas Electron Multiplier (GEM) is analyzed using ANSYS and Garfield++ software coupling. Essential electron transport properties for several gas mixtures were computed as a function of varying electric and magnetic field using Garfield++ and Magboltz.

  13. Final Hanford Site Transuranic (TRU) Waste Characterization Qualit Assurance Project Plan

    GREAGER, T.M.

    1999-01-01

    The Transuranic Waste Characterization Quality Assurance Program Plan required each U.S. Department of Energy (DOE) site that characterizes transuranic waste to be sent the Waste Isolation Pilot Plan that addresses applicable requirements specified in the quality assurance project plan (QAPP)

  14. Fundamental remote sensing science research program. Part 1: Scene radiation and atmospheric effects characterization project

    Murphy, R. E.; Deering, D. W.

    1984-01-01

    Brief articles summarizing the status of research in the scene radiation and atmospheric effect characterization (SRAEC) project are presented. Research conducted within the SRAEC program is focused on the development of empirical characterizations and mathematical process models which relate the electromagnetic energy reflected or emitted from a scene to the biophysical parameters of interest.

  15. Tank characterization project (TWRS) process engineering data management plan

    Adams, M.R.

    1997-01-01

    The Tank Characterization Data Management (TCDM) system provides customers and users with data and information of known and acceptable quality when they are needed, in the form they are needed, and at a reasonable cost. The TCDM mission will be accomplished by the following: (1) maintaining and managing tank characterization data and information based on business needs and objectives including transfer of ownership to future contractors; (2) capturing data where it originates and entering it only once to control data consistency, electronic data and information management shall be emphasized to the extent practicable; (3) establishing data quality standards, and managing and certifying databases and data sources against these standards to maintain the proper level of data and information quality consistent with the importance of the data and information, data obtained at high cost with significant implications to decision making regarding tank safety and/or disposal will be maintained and managed at the highest necessary levels of quality; (4) establishing and enforcing data management standards for the Tank Characterization Database (TCD) and supporting data sources including providing mechanisms for discovering and correcting data errors before they propagate; (5) emphasizing electronic data sharing with all authorized users, customers, contractors, and stakeholders to the extent practicable; (6) safeguarding data and information from unauthorized alteration or destruction; (7) providing standards for electronic information deliverables to subcontractors and vendors to achieve uniformity in electronic data management; and (8) investing in new technology (hardware and/or software) as prudent and necessary to accomplish the mission in an efficient and effective manner

  16. Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair

    Miyaji, E.N.; Johnson, R.T.; Downes, C.S.; Eveno, E.; Mezzina, M.; Sarasin, A.; Menck, C.F.M.

    2000-01-01

    Using a positive selection system for isolating DNA replication and repair related mutants, we isolated a clone from a rat kangaroo cell line (PtK2) that has increased sensitivity to UV light. Characterization of this clone indicated normal post-replication repair after UV irradiation, and normal removal rates of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts by excision repair. However, this cell line has decreased ability to make early incisions on damaged DNA, po...

  17. An integrated in-line fluid characterization system for industrial applications(In-situ fluid mechanics experiments)

    Johan, Wiklund; Reinhardt, Kotze; Beat, Birkhofer; Stefano, Ricci; Valentino, Meacci; Mats, Stading; Rainer, Haldenwang; SP-Technical Research Institute of Sweden; FPRC, Cape Peninsula University of Technology; Sika Services AG; Information Engineering Department - University of Florence; Information Engineering Department - University of Florence; SP-Technical Research Institute of Sweden; FPRC, Cape Peninsula University of Technology

    2015-01-01

    In this work we have presented the world's first commercially available embedded in-line fluids characterization system, "Flow-Viz". It has been specifically designed for the non-invasive, in-line, continuous, real-time velocity profile and rheological assessment of opaque, non-Newtonian industrial fluids. The Flow-Viz system has been successfully installed in pilot plants of international companies and used also for academic research. The technology has been applied to a wide range of fluids...

  18. Multiteide Project: Multiparametric characterization of the activity of Teide-Pico Viejo volcanic system

    Domínguez Cerdeña, Itahiza; Villasante-Marcos, Victor; Meletlidis, Stavros; Sainz-Maza, Sergio; Abella, Rafael; Torres, Pedro A.; Sánchez, Nieves; Luengo-Oroz, Natividad; José Blanco, María; García-Cañada, Laura; Pereda de Pablo, Jorge; Lamolda, Héctor; Moure, David; Del Fresno, Carmen; Finizola, Anthony; Felepto, Alicia

    2017-04-01

    Teide-Pico Viejo complex stands for one of the major natural volcanic hazards in the Canary Islands, due to the expected types of eruptions in the area and the high number of inhabitants in Tenerife Island. Therefore, it is necessary to have a volcanic alert system able to afford a precise assessment of the current state of the complex. For this purpose, the knowledge of the expected signals at each volcanic activity level is required. Moreover, the external effects that can affect the measurements shall be distinguished, external influences as the atmosphere are qualitatively known but have not been quantified yet. The objective of the project is to collect, analyze and jointly and continuously evaluate over time geophysical, geodetic, geochemical and meteorological data from the Teide-Pico Viejo complex and its surroundings. A continuous multiparametric network have been deployed in the area, which, together with the data provided by the Volcano Monitoring Network of the Instituto Geográfico Nacional (IGN) and data from other institutions will provide a comprehensive set of data with high resolution in both space and time. This multiparametric network includes a seismic array, two self-potential lines for continuous measurements, five magnetometers and two weather stations. The network will be complemented with 8 CGPS stations, one tiltmeter, 10 seismic stations, and four thermometric stations on the fumaroles of Teide volcano that IGN already manage in Tenerife. The data will be completed with the results from different repeated surveys of self potential, soil temperature and CO2 diffuse flux in several pre-established areas on top of Teide throughout the entire duration of project. During the project, new computation tools will be developed to study the correlation between the different parameters analyzed. The results obtained will characterize the possible seasonal fluctuations of each parameter and the variations related to meteorological phenomena. In

  19. HB-Line Special Nuclear Material Campaigns: Model-Based Project Management

    CHANG, ROBERT

    2004-01-01

    This study is to show how a model was used to enable management to better estimate production capabilities to ensure contract milestones/commitments are met, to cope with fast changing project baselines and project missions, to ensure the project will meet the negotiated throughput, and to eliminate unnecessary but costly design changes

  20. Tank Vapor Characterization Project: Annual status report for FY 1996

    Silvers, K.L.; Fruchter, J.S.; Huckaby, J.L.; Almeida, T.L.; Evans, J.C. Jr.; Pool, K.H.; Simonen, C.A.; Thornton, B.M.

    1997-01-01

    In Fiscal Year 1996, staff at the Vapor Analytical Laboratory at Pacific Northwest National Laboratory performed work in support of characterizing the vapor composition of the headspaces of radioactive waste tanks at the Hanford Site. Work performed included support for technical issues and sampling methodologies, upgrades for analytical equipment, analytical method development, preparation of unexposed samples, analyses of tank headspaces samples, preparation of data reports, and operation of the tank vapor database. Progress made in FY 1996 included completion and issuance of 50 analytical data reports. A sampling system comparison study was initiated and completed during the fiscal year. The comparison study involved the vapor sampling system (VSS), a truck-based system, and the in situ vapor sampling system (ISVS), a cart-based system. Samples collected during the study were characterized for inorganic, permanent gases, total non-methane organic compounds and organic speciation by SUMMA trademark and TST methods. The study showed comparable sampling results between the systems resulting in the program switching from the VSS to the less expensive ISVS methodology in late May 1996. A temporal study was initiated in January 1996 in order to understand the influences seasonal temperatures changes have on the vapors in the headspace of Hanford waste tanks. A holding time study was initiated in the fourth quarter of FY 1996. Samples were collected from tank S-102 and rushed to the laboratory for time zero analysis. Additional samples will be analyzed at 1, 2, 4, 8, 16, and 32 weeks

  1. Characterization of cetuximab F{sub c/2} dimers by off-line CZE-MS

    François, Yannis-Nicolas, E-mail: yfrancois@unistra.fr [Laboratoire de Spectrométrie de Masse des Interactions et des Systèmes (LSMIS), UDS-CNRS UMR 7140, Université de Strasbourg, Strasbourg (France); Biacchi, Michael; Said, Nassur; Renard, Charly [Laboratoire de Spectrométrie de Masse des Interactions et des Systèmes (LSMIS), UDS-CNRS UMR 7140, Université de Strasbourg, Strasbourg (France); Beck, Alain [Centre d' immunologie Pierre Fabre, Saint-Julien-en-Genevois (France); Gahoual, Rabah [Laboratoire de Spectrométrie de Masse des Interactions et des Systèmes (LSMIS), UDS-CNRS UMR 7140, Université de Strasbourg, Strasbourg (France); Laboratory of Bioanalytical Chemistry and Spectroscopy, Vrije Universiteit, Amsterdam (Netherlands); Leize-Wagner, Emmanuelle [Laboratoire de Spectrométrie de Masse des Interactions et des Systèmes (LSMIS), UDS-CNRS UMR 7140, Université de Strasbourg, Strasbourg (France)

    2016-02-18

    Monoclonal antibody (mAb) therapeutics attract the largest concern due to their strong therapeutic potency and specificity. The Fc region of mAbs is common to many new biotherapeutics as biosimilar, antibody drug conjugate or fusion protein. Fc region has consequences for Fc-mediated effector functions that might be desirable for therapeutic applications. As a consequence, there is a continuous need for improvement of analytical methods to enable fast and accurate characterization of biotherapeutics. Capillary zone electrophoresis-Mass spectrometry couplings (CZE-MS) appear really attractive methods for the characterization of biological samples. In this report, we used CZE-MS systems developed in house and native MS infusion to allow precise middle-up characterization of F{sub c/2} variant of cetuximab. Molecular weights were measured for three F{sub c/2} charge variants detected in the CZE separation of cetuximab subunits. Two F{sub c/2} C-terminal lysine variants were identified and separated. As the aim is to understand the presence of three peaks in the CZE separation for two F{sub c/2} subunits, we developed a strategy using CZE-UV/MALDI-MS and CZE-UV/ESI-MS to evaluate the role of N-glycosylation and C-terminal lysine truncation on the CZE separation. The chemical structure of N-glycosylation expressed on the Fc region of cetuximab does not influence CZE separation while C-terminal lysine is significantly influencing separation. In addition, native MS infusion demonstrated the characterization of F{sub c/2} dimers at pH 5.7 and 6.8 and the first separation of these dimers using CZE-MS. - Highlights: • We confirm the power of off-line CE/MS coupling for the separation of mAb isoforms. • We demonstrate the role of C-terminal lysine truncation in the separation process. • We demonstrate the presence of non-covalent interactions between Fc/2 subunit. • We identify Fc/2 homo- and heterodimers.

  2. Experimental characterization of the flux-line lattice in superconducting V3Si

    Christen, D.K.; Kerchner, H.R.; Narayan, J.; Chang, Y.K.; Larson, B.C.; Sekula, S.T.

    1982-01-01

    Several microscopic properties of the flux-line lattice (FLL) in three separate single crystals of V 3 Si have been investigated by means of small-angle neutron diffraction. These low-field FLL characterizations have been correlated with the following material and superconducting properties: (1) the real crystal symmetry parallel to the applied magnetic field; (2) the micro-structure as determined by TEM; (3) magnetic irreversibilities in the mixed state; (4) reversible flux-line motion in ac response; and (5) martensitic structural transformation observed by x-ray diffraction. The three samples, V 3 Si-MP3, -MP4, and -MP5 possessed different defect structures, and this was manifested foremost in the FLL perfection. At low field (B 0 K, only MP3, which is free of second-phase precipitates, showed a highly resolved FLL. Sample MP5 contains a low density of small (200 A) coherent precipitates, and revealed well-defined FLL Bragg peaks for B greater than or equal to 0.5 T, but a highly mosaic, nearly polycrystalline FLL at lower fields. Sample MP4 contained large (500 to 1000 A) incoherent precipitates, and showed only a polycrystalline FLL at low field. In both MP3 and MP5, distinct anisotropic correlations were observed between the FLL morphology and the real-crystal direction along the applied field. The FLL perfection was strongly dependent on the growth history. The peak width history dependence for two different scattering geometries can be qualitatively modeled by proposed flux-pinning mechanisms. Quantitative comparisons with critical current measurements, however, are not totally reconcilable

  3. Delivering below the line in project management – A conceptual exploration

    Udechukwu Ojiako

    2013-06-01

    Design/Methodology/Approach: The study is conceptual in nature and draws upon literature on complexity theory, expectations theories and stakeholder theory. Findings: The paper finds that there is a need to articulate a different assessment of project outcomes than has been traditionally undertaken. Such assessments may emphasise the perceptions of project clients formed through their experience of projects throughout the project lifecycle. Implications: This paper raises questions on whether the outcomes of projects are best ‘measured’ or ‘assessed’.

  4. Transmission line pulse system for avalanche characterization of high power semiconductor devices

    Riccio, Michele; Ascione, Giovanni; De Falco, Giuseppe; Maresca, Luca; De Laurentis, Martina; Irace, Andrea; Breglio, Giovanni

    2013-05-01

    Because of the increasing in power density of electronic devices for medium and high power application, reliabilty of these devices is of great interest. Understanding the avalanche behaviour of a power device has become very important in these last years because it gives an indication of the maximum energy ratings which can be seen as an index of the device ruggedness. A good description of this behaviour is given by the static IV blocking characteristc. In order to avoid self heating, very relevant in high power devices, very short pulses of current have to be used, whose value can change from few milliamps up to tens of amps. The most used method to generate short pulses is the TLP (Transmission Line Pulse) test, which is based on charging the equivalent capacitance of a transmission line to high value of voltage and subsequently discharging it onto a load. This circuit let to obtain very short square pulses but it is mostly used for evaluate the ESD capability of semiconductor and, in this environment, it generates pulses of low amplitude which are not high enough to characterize the avalanche behaviour of high power devices . Advanced TLP circuit able to generate high current are usually very expensive and often suffer of distorption of the output pulse. In this article is proposed a simple, low cost circuit, based on a boosted-TLP configuration, which is capable to produce very square pulses of about one hundreds of nanosecond with amplitude up to some tens of amps. A prototype is implemented which can produce pulses up to 20A of amplitude with 200 ns of duration which can characterize power devices up to 1600V of breakdown voltage. Usage of microcontroller based logic make the circuit very flexible. Results of SPICE simulation are provided, together with experimental results. To prove the effectiveness of the circuit, the I-V blocking characteristics of two commercial devices, namely a 600V PowerMOS and a 1200V Trench-IGBT, are measured at different

  5. Information Theoretic Characterization of Physical Theories with Projective State Space

    Zaopo, Marco

    2015-08-01

    Probabilistic theories are a natural framework to investigate the foundations of quantum theory and possible alternative or deeper theories. In a generic probabilistic theory, states of a physical system are represented as vectors of outcomes probabilities and state spaces are convex cones. In this picture the physics of a given theory is related to the geometric shape of the cone of states. In quantum theory, for instance, the shape of the cone of states corresponds to a projective space over complex numbers. In this paper we investigate geometric constraints on the state space of a generic theory imposed by the following information theoretic requirements: every non completely mixed state of a system is perfectly distinguishable from some other state in a single shot measurement; information capacity of physical systems is conserved under making mixtures of states. These assumptions guarantee that a generic physical system satisfies a natural principle asserting that the more a state of the system is mixed the less information can be stored in the system using that state as logical value. We show that all theories satisfying the above assumptions are such that the shape of their cones of states is that of a projective space over a generic field of numbers. Remarkably, these theories constitute generalizations of quantum theory where superposition principle holds with coefficients pertaining to a generic field of numbers in place of complex numbers. If the field of numbers is trivial and contains only one element we obtain classical theory. This result tells that superposition principle is quite common among probabilistic theories while its absence gives evidence of either classical theory or an implausible theory.

  6. Volkov transform generalized projection algorithm for attosecond pulse characterization

    Keathley, P D; Bhardwaj, S; Moses, J; Laurent, G; Kärtner, F X

    2016-01-01

    An algorithm for characterizing attosecond extreme ultraviolet pulses that is not bandwidth-limited, requires no interpolation of the experimental data, and makes no approximations beyond the strong-field approximation is introduced. This approach fully incorporates the dipole transition matrix element into the retrieval process. Unlike attosecond retrieval methods such as phase retrieval by omega oscillation filtering (PROOF), or improved PROOF, it simultaneously retrieves both the attosecond and infrared (IR) pulses, without placing fundamental restrictions on the IR pulse duration, intensity or bandwidth. The new algorithm is validated both numerically and experimentally, and is also found to have practical advantages. These include an increased robustness to noise, and relaxed requirements for the size of the experimental dataset and the intensity of the streaking pulse. (paper)

  7. The Genomes On Line Database (GOLD) in 2009: status of genomic and metagenomic projects and their associated metadata

    Liolios, Konstantinos; Chen, I-Min A.; Mavromatis, Konstantinos; Tavernarakis, Nektarios; Hugenholtz, Philip; Markowitz, Victor M.; Kyrpides, Nikos C.

    2010-01-01

    The Genomes On Line Database (GOLD) is a comprehensive resource for centralized monitoring of genome and metagenome projects worldwide. Both complete and ongoing projects, along with their associated metadata, can be accessed in GOLD through precomputed tables and a search page. As of September 2009, GOLD contains information for more than 5800 sequencing projects, of which 1100 have been completed and their sequence data deposited in a public repository. GOLD continues to expand, moving toward the goal of providing the most comprehensive repository of metadata information related to the projects and their organisms/environments in accordance with the Minimum Information about a (Meta)Genome Sequence (MIGS/MIMS) specification. GOLD is available at: http://www.genomesonline.org and has a mirror site at the Institute of Molecular Biology and Biotechnology, Crete, Greece, at: http://gold.imbb.forth.gr/ PMID:19914934

  8. Yucca Mountain Site Characterization Project Bibliography, July--December 1994: An update

    NONE

    1995-03-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Charactrization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy`s Science and Technology Database from July 1, 1994 through December 31, 1994. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers, and articles are included in the sponsoring organization`s list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it.

  9. Yucca Mountain Site Characterization Project Bibliography, July, December 194: An update

    1995-03-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Charactrization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy's Science and Technology Database from July 1, 1994 through December 31, 1994. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers, and articles are included in the sponsoring organization's list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it

  10. Sidney-North Yuma 230-kV Transmission Line Project, Colorado and Nebraska

    1991-06-01

    This report describes the need for a 230-kV overhead transmission line to supply power from Sidney, Nebraska to eastern Colorado. The alternative scenario compared to construction of the line is No Action. Rejected alternatives include underground lines and different routing paths, with a possible extension to the Sterling area. Both scenarios are evaluated for environmental effects, cost, and consequences for the eastern Colorado region. The proposed route is determined to be the environmentally preferred choice. 120 refs., 6 figs., 13 tabs. (MHB)

  11. Characterizing the impact of projected changes in climate and ...

    The impact of climate change on human and environmental health is of critical concern. Population exposures to air pollutants both indoors and outdoors are influenced by a wide range of air quality, meteorological, behavioral, and housing-related factors, many of which are also impacted by climate change. An integrated methodology for modeling changes in human exposures to tropospheric ozone (O3) owing to potential future changes in climate and demographics was implemented by linking existing modeling tools for climate, weather, air quality, population distribution, and human exposure. Human exposure results from the Air Pollutants Exposure Model (APEX) for 12 US cities show differences in daily maximum 8-h (DM8H) exposure patterns and levels by sex, age, and city for all scenarios. When climate is held constant and population demographics are varied, minimal difference in O3 exposures is predicted even with the most extreme demographic change scenario. In contrast, when population is held constant, we see evidence of substantial changes in O3 exposure for the most extreme change in climate. Similarly, we see increases in the percentage of the population in each city with at least one O3 exposure exceedance above 60 p.p.b and 70 p.p.b thresholds for future changes in climate. For these climate and population scenarios, the impact of projected changes in climate and air quality on human exposure to O3 are much larger than the impacts of changing demographics.

  12. Construction, characterization, and complementation of a conditional-lethal DNA topoisomerase IIalpha mutant human cell line.

    Carpenter, Adam J; Porter, Andrew C G

    2004-12-01

    DNA Topoisomerase IIalpha (topoIIalpha) is a DNA decatenating enzyme, abundant constituent of mammalian mitotic chromosomes, and target of numerous antitumor drugs, but its exact role in chromosome structure and dynamics is unclear. In a powerful new approach to this important problem, with significant advantages over the use of topoII inhibitors or RNA interference, we have generated and characterized a human cell line (HTETOP) in which >99.5% topoIIalpha expression can be silenced in all cells by the addition of tetracycline. TopoIIalpha-depleted HTETOP cells enter mitosis and undergo chromosome condensation, albeit with delayed kinetics, but normal anaphases and cytokineses are completely prevented, and all cells die, some becoming polyploid in the process. Cells can be rescued by expression of topoIIalpha fused to green fluorescent protein (GFP), even when certain phosphorylation sites have been mutated, but not when the catalytic residue Y805 is mutated. Thus, in addition to validating GFP-tagged topoIIalpha as an indicator for endogenous topoIIalpha dynamics, our analyses provide new evidence that topoIIalpha plays a largely redundant role in chromosome condensation, but an essential catalytic role in chromosome segregation that cannot be complemented by topoIIbeta and does not require phosphorylation at serine residues 1106, 1247, 1354, or 1393.

  13. Weld-Windsor 115-kV Transmission Line Project, Weld County, Colorado

    1996-05-01

    The Western Area Power Administration is proposing to rebuild a 3.0 mile segment of the existing Flatiron-Weld 115-kV transmission line in Weld County. The line would be reconductored with new conductor on new wood pole double circuit structures. The new structures would support a double circuit transmission line configuration. The first circuit would be owned by Western and the second by Public Service Company of Colorado (PSCO). Alternatives considered included no action, constructing PSCO's circuit on new right-of-way, and reconductoring Western's existing line on the same structures. The proposed action was selected because it provided an opportunity to share structures with PSCO and, overall, would minimize costs and environmental impacts. The environmental assessment identifies minor effects on existing natural or human resources and minor benefits for agricultural operations

  14. Final report on the Background Soil Characterization Project at the Oak Ridge Reservation, Oak Ridge, Tennessee

    Watkins, D.R.; Ammons, J.T.; Branson, J.L.

    1993-10-01

    This report presents, evaluates, and documents data and results obtained in the Background Soil Characterization Project (BSCP). It is intended to be a stand-alone document for application and use in structuring and conducting remedial investigation and remedial action projects in the Environmental Restoration (ER) Program. The objectives of the BSCP consist of the following: determine background concentrations of organics, metals, and radionuclides in natural soils that are key to environmental restoration projects; provide remediation projects with 100% validated data on background concentrations, which are technically and legally defensible; and quantify baseline risks from background constituents for comparison of risks associated with contaminated sites

  15. Characterization of four clones derived from human adenocarcinoma cell line, HT29, and analysis of their response to sodium butyrate

    Štokrová, Jitka; Šloncová, Eva; Sovová, Vlasta; Kučerová, Dana; Žíla, Vojtěch; Turečková, Jolana; Vojtěchová, Martina; Korb, Jan; Tuháčková, Zdena

    2006-01-01

    Roč. 28, č. 2 (2006), s. 559-565 ISSN 1019-6439 R&D Projects: GA AV ČR(CZ) KJB5052302 Institutional research plan: CEZ:AV0Z50520514 Keywords : adenocarcinoma * HT29 cell line * ultrastructure analysis Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.556, year: 2006

  16. Characterization of prolactin-releasing peptide: Binding, signaling and hormone secretion in rodent pituitary cell lines endogenously expressing its receptor

    Maixnerová, Jana; Špolcová, Andrea; Pýchová, Miroslava; Blechová, Miroslava; Elbert, Tomáš; Řezáčová, M.; Železná, Blanka; Maletínská, Lenka

    2011-01-01

    Roč. 32, č. 4 (2011), s. 811-817 ISSN 0196-9781 R&D Projects: GA ČR GAP303/10/1368 Institutional research plan: CEZ:AV0Z40550506 Keywords : PrRP * pituitary cell lines * food intake Subject RIV: CC - Organic Chemistry Impact factor: 2.434, year: 2011

  17. Sierra Pacific Power Company Alturas Transmission Line Project, Final Environmental Impact Report/Environmental Impact Statement. Volume 3: Appendices

    1995-11-01

    Sierra Pacific Power Company has proposed the construction and operation of a 345,000 volt overhead electric power transmission line from Alturas, California to Reno, Nevada. This Environmental Impact Report/Environmental Impact Statement will assess the potential environmental impacts of the proposed project and alternatives. This report contains Appendices A--I which contain the following: glossary/abbreviations; scoping report; structure coordinate summary; air quality; biological resources; geology; noise; visual contrast rating forms; and cultural resources

  18. Influence of Projection Operator on Oxygen Line Shapes and its effect on Rosseland-Mean Opacity in Stellar Interiors

    Gomez, Thomas; Nagayama, Taisukue; Kilcrease, David; Hansen, Stephanie; Montgomery, Mike; Winget, Don

    2018-01-01

    The Rosseland-Mean opacity (RMO) is an important quantity in determining radiation transport through stars. The solar-convection-zone boundary predicted by the standard solar model disagrees with helioseismology measurements by many sigma; a 14% increase in the RMO would resolve this discrepancy. Experiments at Sandia National Laboratories are now measuring iron opacity at solar-interior conditions, and significant discrepancies are already observed. Highly-ionized oxygen is one of the dominant contributions to the RMO. The strongest line, Lyman alpha, is at the peak of the Rosseland weighting function. The accuracy of line-broadening calculations has been called into question due to various experimental results and comparisons between theory. We have developed an ab-initio calculation to explore different physical effects, our current focus is treating penetrating collisions explicitly. The equation of motion used to calculate line shapes within the relaxation and unified theories includes a projection operator, which performs an average over plasma electron states; this is neglected due to past calculations approximate treatment of penetrations. We now include this projection term explicitly, which results in a significant broadening of spectral lines from highly-charged ions (low-Z elements are not much affected). The additional broadening raises the O Ly-alpha wing opacity by a factor of 5; we examine the consequences of this additional broadening on the Rosseland mean.

  19. Implementation of an integrated on-line process surveillance and diagnostic system at the Halden reactor project: MOAS

    Kim, I.S.; Grini, R.-E.; Nilsen, S.

    2001-01-01

    MOAS is an integrated on-line process surveillance and diagnostic system that uses several different models for knowledge acquisition and diagnostic reasoning, such as goal-tree success-tree model, process monitor trees, and sensor failure diagnosis trees. Within these models, the knowledge of the process and its operation, including deep knowledge, like mass balance or controller algorithm, is incorporated. During an extensive review, made as part of the integrated diagnosis system project of the Halden reactor project, MOAS (Maryland Operator Advisory System) was identified as one of the most thorough systems developed thus far. MOAS encompasses diverse functional aspects that are required for an effective process disturbance management: (1) intelligent process monitoring and alarming, (2) on-line sensor data validation and sensor failure diagnosis, (3) on-line hardware (besides sensors) failure diagnosis, and (4) real-time corrective measure synthesis. The MOAS methodology was used for the NORS (Nokia Research Simulator) process at the Halden man-machine laboratory HAMMLAB of the OECD Halden reactor project. The performance tests of MOAS, implemented in G2 real-time expert system shell, show that MOAS successfully carries out its intended functions, i.e. quickly recognizing an occurring disturbance, correctly diagnosing its cause, and presenting advice on its control to the operator. The lessons learned and insights gained during the implementation and performance tests also are discussed

  20. Selection and characterizations of radiation-induced salinity-tolerant lines in rice

    Lee, I.S.; Kim, D.S.; Lee, S.J.; Song, H.S.; Lim, Y.P.; Lee, Y.I.

    2003-01-01

    NaCl tolerant cell lines were selected from irradiated callus, which were generated from seed cultures. M 1 -regenerates were obtained from the salt-tolerant callus cultured on the auxin-free medium for 30 days. Some regenerants were more tolerant than the parent variety (Dongjinbyeo) on a medium containing 0.75 % NaCl. Seeds (M 3 5,000 lines) derived from M 2 lines were grown to the 3 leaf stage. M 3 lines were soaked with a 0.75 % salt solution for 3 weeks and 350 salt-tolerant genotypes were selected. Among the M 3 350 lines, forty tolerant lines were selected from a saline field (10~14 mS) near the sea coast. Of the forty lines, two lines (18-1 and 50-1) showed more improved plant height, panicle length, tillering number, spikelet number and yield than those of the original variety. Thirty primers were screened and two RAPD markers were identified, which appeared in both the salt-tolerant lines (18-1 and 50-1). From DNA-hybridization experiments, it appeared that the fragment arose from the middle-repetitive copy sequences. The transcript involved in the marker showed a higher expression in the salt-tolerant lines than the sensitive lines. The salt-tolerant lines would be useful as a resource for salt-tolerant breeding. (author)

  1. THE LICK AGN MONITORING PROJECT: REVERBERATION MAPPING OF OPTICAL HYDROGEN AND HELIUM RECOMBINATION LINES

    Bentz, Misty C.; Walsh, Jonelle L.; Barth, Aaron J.; Thornton, Carol E.; Yoshii, Yuzuru; Sakata, Yu; Minezaki, Takeo; Woo, Jong-Hak; Malkan, Matthew A.; Wang, Xiaofeng; Steele, Thea N.; Silverman, Jeffrey M.; Serduke, Frank J. D.; Li, Weidong; Lee, Nicholas; Treu, Tommaso; Street, Rachel A.; Hidas, Marton G.; Hiner, Kyle D.; Greene, Jenny E.

    2010-01-01

    We have recently completed a 64-night spectroscopic monitoring campaign at the Lick Observatory 3 m Shane telescope with the aim of measuring the masses of the black holes in 12 nearby (z 6 -10 7 M sun and also the well-studied nearby active galactic nucleus (AGN) NGC 5548. Nine of the objects in the sample (including NGC 5548) showed optical variability of sufficient strength during the monitoring campaign to allow for a time lag to be measured between the continuum fluctuations and the response to these fluctuations in the broad Hβ emission, which we have previously reported. We present here the light curves for the Hα, Hγ, He II λ4686, and He I λ5876 emission lines and the time lags for the emission-line responses relative to changes in the continuum flux. Combining each emission-line time lag with the measured width of the line in the variable part of the spectrum, we determine a virial mass of the central supermassive black hole from several independent emission lines. We find that the masses are generally consistent within the uncertainties. The time-lag response as a function of velocity across the Balmer line profiles is examined for six of the AGNs. We find similar responses across all three Balmer lines for Arp 151, which shows a strongly asymmetric profile, and for SBS 1116+583A and NGC 6814, which show a symmetric response about zero velocity. For the other three AGNs, the data quality is somewhat lower and the velocity-resolved time-lag response is less clear. Finally, we compare several trends seen in the data set against the predictions from photoionization calculations as presented by Korista and Goad. We confirm several of their predictions, including an increase in responsivity and a decrease in the mean time lag as the excitation and ionization level for the species increases. Specifically, we find the time lags of the optical recombination lines to have weighted mean ratios of τ(Hα):τ(Hβ):τ(Hγ):τ(He I):τ(He II) = 1

  2. Managing your engineering consultants: Steps for simultaneously improving operations, project implementation, and your bottom line

    Kirchen, E.R.; Perilloux, B.L.

    1997-01-01

    The domestic oil and gas industry has responded to depleting reserves and increasing operating costs by downsizing the overhead required to maintain production and processing facilities. For many companies this downsizing has resulted in a reduced in-house engineering staff and a greater reliance on consulting engineering services. To get the most benefit from consulting engineering companies, the partnership between consultants and the oil and gas company needs to be carefully considered. Unfortunately, these partnerships are often developed at the home office with visionary goals in mind, only to be implemented reluctantly on a local level. A better strategy is to implement partnering tools on the local level and allow these partnerships to develop naturally, and at times, uniquely, at each location. The following such tools detailed in this paper are: manpower leveraging -- using field-trained consulting engineers to address project design/implementation and field/construction support so that the operating company's engineers may focus on management and detailed development of high-return projects; enhanced project scope and design review -- developing and reviewing project scope(s) and preliminary engineering designs to minimize engineering/construction costs as well as optimize the operability and constructability of the project; and consulting rate standardization -- understanding and structuring the consultant's rates so that neither side is exploited and so that the project is staffed in the interest of project execution and not maximum profits for the consultant

  3. Parallelism at Cern: real-time and off-line applications in the GP-MIMD2 project

    Calafiura, P.

    1997-01-01

    A wide range of general purpose high-energy physics applications, ranging from Monte Carlo simulation to data acquisition, from interactive data analysis to on-line filtering, have been ported, or developed, and run in parallel on IBM SP-2 and Meiko CS-2 CERN large multi-processor machines. The ESPRIT project GP-MIMD2 has been a catalyst for the interest in parallel computing at CERN. The project provided the 128 processor Meiko CS-2 system that is now succesfully integrated in the CERN computing environment. The CERN experiment NA48 was involved in the GP-MIMD2 project since the beginning. NA48 physicists run, as part of their day-to-day work, simulation and analysis programs parallelized using the message passing interface MPI. The CS-2 is also a vital component of the experiment data acquisition system and will be used to calibrate in real-time the 13000 channels liquid krypton calorimeter. (orig.)

  4. THE LICK AGN MONITORING PROJECT 2011: SPECTROSCOPIC CAMPAIGN AND EMISSION-LINE LIGHT CURVES

    Barth, Aaron J. [Department of Physics and Astronomy, 4129 Frederick Reines Hall, University of California, Irvine, CA, 92697-4575 (United States); Bennert, Vardha N. [Physics Department, California Polytechnic State University, San Luis Obispo, CA 93407 (United States); Canalizo, Gabriela [Department of Physics and Astronomy, University of California, Riverside, CA 92521 (United States); Filippenko, Alexei V.; Li, Weidong [Department of Astronomy, University of California, Berkeley, CA 94720-3411 (United States); Gates, Elinor L. [Lick Observatory, P.O. Box 85, Mount Hamilton, CA 95140 (United States); Greene, Jenny E. [Department of Astrophysical Sciences, Princeton University, Princeton, NJ 08544 (United States); Malkan, Matthew A.; Treu, Tommaso [Department of Physics and Astronomy, University of California, Los Angeles, CA 90095-1547 (United States); Pancoast, Anna [Department of Physics, University of California, Santa Barbara, CA 93106 (United States); Sand, David J. [Texas Tech University, Physics Department, Box 41051, Lubbock, TX 79409-1051 (United States); Stern, Daniel [Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Boulevard, Pasadena, CA 91109 (United States); Woo, Jong-Hak [Astronomy Program, Department of Physics and Astronomy, Seoul National University, Seoul 151-742 (Korea, Republic of); Assef, Roberto J. [Núcleo de Astronomía de la Facultad de Ingeniería, Universidad Diego Portales, Av. Ejército Libertador 441, Santiago (Chile); Bae, Hyun-Jin [Department of Astronomy and Center for Galaxy Evolution Research, Yonsei University, Seoul 120-749 (Korea, Republic of); Brewer, Brendon J. [Department of Statistics, The University of Auckland, Private Bag 92019, Auckland 1142 (New Zealand); Cenko, S. Bradley [Astrophysics Science Division, NASA Goddard Space Flight Center, MC 661, Greenbelt, MD 20771 (United States); and others

    2015-04-15

    In the Spring of 2011 we carried out a 2.5 month reverberation mapping campaign using the 3 m Shane telescope at Lick Observatory, monitoring 15 low-redshift Seyfert 1 galaxies. This paper describes the observations, reductions and measurements, and data products from the spectroscopic campaign. The reduced spectra were fitted with a multicomponent model in order to isolate the contributions of various continuum and emission-line components. We present light curves of broad emission lines and the active galactic nucleus (AGN) continuum, and measurements of the broad Hβ line widths in mean and rms spectra. For the most highly variable AGNs we also measured broad Hβ line widths and velocity centroids from the nightly spectra. In four AGNs exhibiting the highest variability amplitudes, we detect anticorrelations between broad Hβ width and luminosity, demonstrating that the broad-line region “breathes” on short timescales of days to weeks in response to continuum variations. We also find that broad Hβ velocity centroids can undergo substantial changes in response to continuum variations; in NGC 4593, the broad Hβ velocity shifted by ∼250 km s{sup −1} over a 1 month period. This reverberation-induced velocity shift effect is likely to contribute a significant source of confusion noise to binary black hole searches that use multi-epoch quasar spectroscopy to detect binary orbital motion. We also present results from simulations that examine biases that can occur in measurement of broad-line widths from rms spectra due to the contributions of continuum variations and photon-counting noise.

  5. Reporter cell lines for the characterization of the interactions between nuclear receptors and endocrine disruptors

    marina egrimaldi

    2015-05-01

    Full Text Available Endocrine-disrupting chemicals (EDCs are exogenous substances interfering with hormone biosynthesis, metabolism, or action, and consequently causing disturbances in the endocrine system. Various pathways are activated by EDCs, including interactions with nuclear receptors (NRs which are primary targets of numerous environmental contaminants.The main NRs targeted by environmental contaminants are the estrogen (ER α, β and the androgen (AR receptors. ERs and AR have pleiotropic regulatory roles in a diverse range of tissues, notably in the mammary gland, the uterus and the prostate. Thus, dysfunctional ERs and AR signaling due to inappropriate exposure to environmental pollutants may lead to hormonal cancers and infertility. The pregnane X receptor (PXR is also recognized by many environmental molecules. PXR has a protective role of the body through its ability to regulate proteins involved in the metabolism, the conjugation and the transport of many exogenous and endogenous compounds. However, the permanent activation of this receptor by xenobiotics may lead to premature drug metabolism, the formation and accumulation of toxic metabolites and defects in hormones homeostasis. The activity of other NRs can also be affected by environmental molecules. Compounds capable of inhibiting or activating the estrogen related (ERRγ, the thyroid hormone (TRα, β, the retinoid X receptors (RXRα, β, γ and peroxisome proliferator-activated (PPAR α, γ receptors have been identified and are highly suspected to promote developmental, reproductive, neurological, or metabolic diseases in humans and wildlife.In this review we provide an overview of reporter cell lines established to characterize the human NR activities of a large panel of EDCs including natural as well as industrial compounds such as pesticides, plasticizers, surfactants, flame retardants and cosmetics.

  6. Characterization of new cell line stably expressing CHI3L1 oncogene

    Chekhonin V. P.

    2011-06-01

    Full Text Available Aim. To characterize the immortalized 293 cell line after stable transfection with human oncogene (CHI3L1. Methods. 293 cells, stably transfected with pcDNA3.1_CHI3L1, and 293 cells, stably transfected with pcDNA3.1 as a negative control, were used throughout all experiments. The clones of CHI3L1-expressing 293 cells and 293 cells, transfected with pcDNA3.1, were analyzed by immunofluorescence and confocal microscopy. Cell proliferation was measured using MTT assay; analyses of ERK1/2 and AKT activation and their cellular localization were performed with anti-phospho-ERK and anti-phospho-AKT antibodies. Specific activation of MAP and PI3 kinases was measured by densitometric analysis of Western-blot signals. Results. The obtained results show quite modest ability of CHI3L1 to stimulate cell growth and reflect rather an improved cellular plating efficiency of the 293 cells stably transfected with pcDNA3.1_CHI3L1 as compared to the 293 cells transfected with an «empty» vector. ERK1/2 and AKT are activated in the 293_CHI3L1 cells. In these cells phosphorylated ERK1/2 were localized in both cell cytoplasm and nuclei while AKT only in cytoplasm. The 293_CHI3L1 cells differed from the 293 cells, transfected with an «empty» vector, in their size and ability to adhere to the culture plates. Conclusions. The overexpression of CHI3L1 is likely to have an important role in tumorigenesis via a mechanism which involves activation of PI3K and ERK1/2 pathways. The tumors which can be induced by orthotopic implantation of the transformed human cells with overexpressed human oncogene CHI3L1 into the rat brain can be used as a target for anticancer drug development.

  7. Characterizing ICF Neutron Scintillation Diagnostics on the nTOF line at SUNY Geneseo

    Lawson-Keister, Pat; Padawar-Curry, Jonah; Visca, Hannah; Fletcher, Kurt; Padalino, Stephen; Sangster, T. Craig; Regan, Sean

    2015-11-01

    Neutron scintillator diagnostics for ICF and HEDP can be characterized using the neutron time-of-flight (nTOF) line on Geneseo's 1.7 MV tandem Pelletron accelerator. Neutron signals can be differentiated from gamma signals by employing coincidence methods. A 1.8-MeV beam of deuterons incident on a deuterated polyethylene target produces neutrons via the 2H(d,n)3He reaction. Neutrons emerging at a lab angle of 88° have an energy of 2.96 MeV; the 3He ions associated with these neutrons are detected at a scattering angle of 43° using a surface barrier detector. The time of flight of the neutron can be measured by using the 3He detection as a ``start'' signal and the scintillation detection as a ``stop'' signal. This time of flight requirement is used to identify the 2.96-MeV neutron signals in the scintillator. To measure the light curve produced by these monoenergetic neutrons, two photomultiplier (PMT) tubes are attached to the scintillator. The full aperture PMT establishes the nTOF coincidence. The other PMT is fitted with a pinhole to collect single events. The time between the full aperture PMT signal and the arrival of the signal in the pinhole PMT is used to determine the light curve for the scintillator. This system will enable the neutron response of various scintillators to be compared. Supported in part by the Department of Energy National Nuclear Security Administration under Award Number DE-NA0001944.

  8. Characterizing the turbulent porosity of stellar wind structure generated by the line-deshadowing instability

    Owocki, Stanley P.; Sundqvist, Jon O.

    2018-03-01

    We analyse recent 2D simulations of the non-linear evolution of the line-deshadowing instability (LDI) in hot-star winds, to quantify how the associated highly clumped density structure can lead to a `turbulent porosity' reduction in continuum absorption and/or scattering. The basic method is to examine the statistical variations of mass column as a function of path length, and fit these to analytic forms that lead to simple statistical scalings for the associated mean extinction. A key result is that one can characterize porosity effects on continuum transport in terms of a single `turbulent porosity length', found here to scale as H ≈ (fcl - 1)a, where fcl ≡ 〈ρ2〉/〈ρ〉2 is the clumping factor in density ρ, and a is the density autocorrelation length. For continuum absorption or scattering in an optically thick layer, we find the associated effective reduction in opacity scales as ˜ 1/√{1+τ_H}, where τH ≡ κρH is the local optical thickness of this porosity length. For these LDI simulations, the inferred porosity lengths are small, only about a couple per cent of the stellar radius, H ≈ 0.02R*. For continuum processes like bound-free absorption of X-rays that are only marginally optically thick throughout the full stellar wind, this implies τH ≪ 1, and thus that LDI-generated porosity should have little effect on X-ray transport in such winds. The formalism developed here could however be important for understanding the porous regulation of continuum-driven, super-Eddington outflows from luminous blue variables.

  9. Yucca Mountain Site Characterization Project bibliography, 1992--1994. Supplement 4

    NONE

    1992-06-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy`s Energy Science and Technology Database from January 1, 1992, through December 31, 1993. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers, and articles are included in the sponsoring organization`s list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it. Earlier information on this project can be found in the first bibliography DOE/TIC-3406, which covers 1977--1985, and its three supplements DOE/OSTI-3406(Suppl.1), DOE/OSTI-3406(Suppl.2), and DOE/OSTI-3406(Suppl.3), which cover information obtained during 1986--1987, 1988--1989, and 1990--1991, respectively. All entries in the bibliographies are searchable online on the NNW database file. This file can be accessed through the Integrated Technical Information System (ITIS) of the US Department of Energy (DOE).

  10. Yucca Mountain Site Characterization Project bibliography, 1992--1993. Supplement 4

    1992-06-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project that was added to the Department of Energy's Energy Science and Technology Database from January 1, 1992, through December 31, 1993. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers, and articles are included in the sponsoring organization's list. Another section contains information about publications on the Energy Science and Technology Database that were not sponsored by the project but have some relevance to it. Earlier information on this project can be found in the first bibliography DOE/TIC-3406, which covers 1977--1985, and its three supplements DOE/OSTI-3406(Suppl.1), DOE/OSTI-3406(Suppl.2), and DOE/OSTI-3406(Suppl.3), which cover information obtained during 1986--1987, 1988--1989, and 1990--1991, respectively. All entries in the bibliographies are searchable online on the NNW database file. This file can be accessed through the Integrated Technical Information System (ITIS) of the US Department of Energy (DOE)

  11. Evaluation of double drop beads pavement edge lines : SPR project description.

    2009-01-01

    In UTCA projects 01465 and 04405, the retroreflectivities and the life cycle costs were measured and compared for standard Alabama Department of Transportation (ALDOT) thermoplastic edge : stripes that contained only one size of retroreflective beads...

  12. Project resumes: biological effects from electric fields associated with high-voltage transmission lines

    None

    1980-01-01

    Abstracts of research projects are presented in the following areas: measurements and special facilities; cellular and subcellular studies; physiology; behavior; environmental effects; modeling, scaling and dosimetry; and high voltage direct current. (ACR)

  13. Participatory ergonomics in industrial engineering projects: The case of a new cheese packaging line work system

    Souza da Conceição, Carolina; Broberg, Ole; Aldrich, Per

    2014-01-01

    The aim of this study is to explore and conceptualize the challenges that ergonomists meet when seeking to introduce PE methods into an industrial engineering design project in order to optimize the new work system.......The aim of this study is to explore and conceptualize the challenges that ergonomists meet when seeking to introduce PE methods into an industrial engineering design project in order to optimize the new work system....

  14. Are you a Project Leader, PSO, GLIMOS?! Are you in line to be one?

    HSE Unit

    2013-01-01

    You can count on a Safety training course to assist you in:   respecting CERN Safety rules and agreements with the Host States;   ensuring that applicable Safety rules/practices are respected or that acceptable Safety measures are known and implemented in your project/experiment;   creating Safety files/folders for your project/experiment;   obtaining HSE Safety Clearance.   Sign in to CTA here.

  15. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1994 quality program status report

    Bolivar, S.L.

    1996-03-01

    This status report is for calendar year 1994. It summarizes the annual activities and accomplishments of the Los Alamos National Laboratory Yucca Mountain Site Characterization Project (YMP or Project) quality assurance program. By identifying the accomplishments of the quality program, a baseline is established that will assist in decision making, improve administrative controls and predictability, and allow us to annually identify adverse trends and to evaluate improvements. This is the fourth annual status report.

  16. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1994 quality program status report

    Bolivar, S.L.

    1996-03-01

    This status report is for calendar year 1994. It summarizes the annual activities and accomplishments of the Los Alamos National Laboratory Yucca Mountain Site Characterization Project (YMP or Project) quality assurance program. By identifying the accomplishments of the quality program, a baseline is established that will assist in decision making, improve administrative controls and predictability, and allow us to annually identify adverse trends and to evaluate improvements. This is the fourth annual status report

  17. The SAURON project - VI. Line strength maps of 48 elliptical and lenticular galaxies

    Kuntschner, Harald; Emsellem, Eric; Bacon, R.; Bureau, M.; Cappellari, Michele; Davies, Roger L.; de Zeeuw, P. T.; Falcon-Barroso, Jesus; Krajnovic, Davor; McDermid, Richard M.; Peletier, Reynier F.; Sarzi, Marc

    2006-01-01

    We present absorption line strength maps of 48 representative elliptical and lenticular galaxies obtained as part of a survey of nearby galaxies using our custom-built integral-field spectrograph, SAURON, operating on the William Herschel Telescope. Using high-quality spectra, spatially binned to a

  18. The SAURON project : XVIII. The integrated UV-line-strength relations of early-type galaxies

    Bureau, Martin; Jeong, Hyunjin; Yi, Sukyoung K.; Schawinski, Kevin; Houghton, Ryan C. W.; Davies, Roger L.; Bacon, Roland; Cappellari, Michele; de Zeeuw, P. Tim; Emsellem, Eric; Falcon-Barroso, Jesus; Krajnovic, Davor; Kuntschner, Harald; McDermid, Richard M.; Peletier, Reynier F.; Sarzi, Marc; Sohn, Young-Jong; Thomas, Daniel; van den Bosch, Remco C. E.; van de Ven, Glenn

    Using far-ultraviolet (FUV) and near-ultraviolet (NUV) photometry from guest investigator programmes on the Galaxy Evolution Explorer (GALEX) satellite, optical photometry from the MDM Observatory and optical integral-field spectroscopy from SAURON, we explore the UV-line-strength relations of the

  19. Derivation and characterization of the NYSCFe003-A human embryonic stem cell line

    Ana Sevilla

    2017-12-01

    Full Text Available The human embryonic stem cell line NYSCFe003-A was derived from a day 5 to day 6 blastocyst in feeder-free and antibiotic free conditions. The blastocyst was voluntarily donated for research as surplus after in vitro fertilization treatment following informed consent. The NYSCFe003-A line expresses all the pluripotency markers and has the potential to differentiate into all three germ layers in vitro. The line presents normal karyotype and is mycoplasma free.

  20. Continuous, environmental radon monitoring program at the Yucca Mountain Site Characterization Project

    Liu, N.; Sorensen, C.D.; Tung, C.H.; Orchard, C.R.

    1995-01-01

    A continuous, environmental radon monitoring program has been established in support of the Department of Energy's (DOE) Yucca Mountain Site Characterization Project (YMP). The monitoring program is to characterize the natural radon emissions at the YMP site, to understand the existing radon concentrations in the environmental background, and to assess and control the potential work exposure. Based upon a study of the monitoring results, this paper presents a preliminary understanding of the magnitudes, characteristics, and exposure levels of radon at the YMP site

  1. Management system information of characterization of the dismantling project of Jose Cabrera

    Gimeno Blesa, M. E.; Martin Palomo, N.; Gomez Rodriguez, C. A.

    2011-01-01

    In the proposed dismantling and decommissioning of the Jose Cabrera NPP is designed and implemented a database of physical and radiological inventory, which provides a powerful tool to optimize the storage, monitoring and control of the characterization data. The database is a useful and reliable management system characterization information that facilitates access and information processing, and ensures their integrity and traceability along of the dismantling project.

  2. Structuring of conductive silver line by electrohydrodynamic jet printing and its electrical characterization

    Lee, Dae-Young; Lee, Jae-Chang; Shin, Yun-Soo; Park, Sung-Eun; Kim, Yong-Jun; Hwang, Jungho; Yu, Tae-U

    2008-01-01

    A set of silver lines with a few hundred nanometers in thickness and with a few hundred micrometers in width were obtained using the electrohydrodynamic jet printing. The lines exhibited about three times higher resistivity (4.8 μΩcm) than that of bulk silver after thermal sintering process. The characteristic impedance of the silver line was about 18 Ω while the value calculated was 20 Ω. This paper demonstrated the possibility of using electrohydrodynamic jet printing of silver nanoparticles to obtain conductive line onto circuit boards.

  3. GGE Biplot projection in discriminating the efficiency of popcorn lines to use nitrogen

    Adriano dos Santos

    Full Text Available ABSTRACT Nitrogen is essential for sustaining life on the planet, and it is the most important nutrient for obtaining high agricultural production. However, their use leads to the release of nitrous oxide with a global warming potential 296 times higher than the CO2 molecule, making it a challenge to reduce their use in agriculture. The objective of this research was to identify efficient popcorn inbred lines and responsive nitrogen use and exhibit a good expansion volume. For this, 29 inbred lines from the Germplasm Collection of Darcy Ribeiro North Fluminense State University (UENF were evaluated at two contrasting levels of nitrogen availability (low and ideal at two representative locations in the north and northwest of the state of Rio de Janeiro, Brazil, arranged in a randomized block design with three replicates. These inbred lines were discriminated against efficient use of nitrogen by multivariate GGE Biplot. Selective accuracy was close to 1, showing that the genotypes were enough to provide contrasting success in selection procedures. The first two main components (PC retained 93.82% of the total variation, and PC1 furnished an information ratio (IR that was unaffected by noise. L77 was the most unstable line, while P7, P2, P6, P3, P5, P4, P9, P10, P8, P9, L70, L74, and L55 were efficient and responsive. The GGE biplot method is recommended for the reliable identification of popcorn lines that are efficient and responsive to the use of nitrogen.

  4. Engineered materials characterization report for the Yucca Mountain Site Characterization Project. Volume 3: Corrosion and data modeling

    Van Konynenburg, R.A.; McCright, R.D.; Roy, A.K.; Jones, D.A.

    1995-08-01

    This three-volume report serves several purposes. The first volume provides an introduction to the engineered materials effort for the Yucca Mountain Site Characterization Project. It defines terms and outlines the history of selection and characterization of these materials. A summary of the recent engineered barrier materials characterization workshop is presented, and the current candidate materials are listed. The second volume tabulates design data for engineered materials, and the third volume is devoted to corrosion data, radiation effects on corrosion, and corrosion modeling. The second and third volumes are intended to be evolving documents, to which new data will be added as they become available from additional studies. The initial version of Volume 3 is devoted to information currently available for environments most similar to those expected in the potential Yucca Mountain repository. This is volume three

  5. Project for a beam line consecrated to soft condensed matter, common heterogeneous materials and non-crystalline materials on soleil

    Ne, F.; Zemb, T.

    1998-01-01

    This project is a part of the 'SOLEIL' synchrotron project. The camera proposed is optimized for small angle x-ray scattering in the domain of soft condensed matter, common heterogeneous materials such as wood, cements, glass, and more generally non-crystalline materials. The beam line is designed to allow a quick succession of different users without time consuming adjustments. Therefore, optical settings are minimized, taking into account the pluri-disciplinary nature of the analysis possibilities. To this end, the technical requirements are as follows. First and essentially, the wave-length has to be fixed and set around 12 keV. Focusing mirrors, optics to sample and sample to detector distances, and the size of the detector allow for a wide range of wave vector to be used. Rejection rate will be lower, and angular dynamical range will be larger than any of the current synchrotron lines. We want this line to be, and to stay, complementary to more specific systems, such as reflectivity experiments or grazing angle scattering experiments. However, we are thinking of an adaptation to ultra small angle scattering mode, based on the Bonse and Hart camera. Such equipment, actually a kind of 'Instamatic' of the reciprocal space, will fulfill to the need of chemical engineers, biophysicists or material scientists interested in hard as well as soft condensed matter. It will allow a large amount of experiments per time unit. (author)

  6. Characterizing Excavation Damaged Zone and Stability of Pressurized Lined Rock Caverns for Underground Compressed Air Energy Storage

    Kim, Hyung-Mok; Rutqvist, Jonny; Jeong, Ju-Hwan; Choi, Byung-Hee; Ryu, Dong-Woo; Song, Won-Kyong

    2013-09-01

    In this paper, we investigate the influence of the excavation damaged zone (EDZ) on the geomechanical performance of compressed air energy storage (CAES) in lined rock caverns. We conducted a detailed characterization of the EDZ in rock caverns that have been excavated for a Korean pilot test program on CAES in (concrete) lined rock caverns at shallow depth. The EDZ was characterized by measurements of P- and S-wave velocities and permeability across the EDZ and into undisturbed host rock. Moreover, we constructed an in situ concrete lining model and conducted permeability measurements in boreholes penetrating the concrete, through the EDZ and into the undisturbed host rock. Using the site-specific conditions and the results of the EDZ characterization, we carried out a model simulation to investigate the influence of the EDZ on the CAES performance, in particular related to geomechanical responses and stability. We used a modeling approach including coupled thermodynamic multiphase flow and geomechanics, which was proven to be useful in previous generic CAES studies. Our modeling results showed that the potential for inducing tensile fractures and air leakage through the concrete lining could be substantially reduced if the EDZ around the cavern could be minimized. Moreover, the results showed that the most favorable design for reducing the potential for tensile failure in the lining would be a relatively compliant concrete lining with a tight inner seal, and a relatively stiff (uncompliant) host rock with a minimized EDZ. Because EDZ compliance depends on its compressibility (or modulus) and thickness, care should be taken during drill and blast operations to minimize the damage to the cavern walls.

  7. Expansion of the On-line Archive "Statistically Downscaled WCRP CMIP3 Climate Projections"

    Brekke, L. D.; Pruitt, T.; Maurer, E. P.; Das, T.; Duffy, P.; White, K.

    2009-12-01

    Presentation highlights status and plans for a public-access archive of downscaled CMIP3 climate projections. Incorporating climate projection information into long-term evaluations of water and energy resources requires analysts to have access to projections at "basin-relevant" resolution. Such projections would ideally be bias-corrected to account for climate model tendencies to systematically simulate historical conditions different than observed. In 2007, the U.S. Bureau of Reclamation, Santa Clara University and Lawrence Livermore National Laboratory (LLNL) collaborated to develop an archive of 112 bias-corrected and spatially disaggregated (BCSD) CMIP3 temperature and precipitation projections. These projections were generated using 16 CMIP3 models to simulate three emissions pathways (A2, A1b, and B1) from one or more initializations (runs). Projections are specified on a monthly time step from 1950-2099 and at 0.125 degree spatial resolution within the North American Land Data Assimilation System domain (i.e. contiguous U.S., southern Canada and northern Mexico). Archive data are freely accessible at LLNL Green Data Oasis (url). Since being launched, the archive has served over 3500 data requests by nearly 500 users in support of a range of planning, research and educational activities. Archive developers continue to look for ways to improve the archive and respond to user needs. One request has been to serve the intermediate datasets generated during the BCSD procedure, helping users to interpret the relative influences of the bias-correction and spatial disaggregation on the transformed CMIP3 output. This request has been addressed with intermediate datasets now posted at the archive web-site. Another request relates closely to studying hydrologic and ecological impacts under climate change, where users are asking for projected diurnal temperature information (e.g., projected daily minimum and maximum temperature) and daily time step resolution. In

  8. Development and characterization of a Psathyrostachys huashanica Keng 7Ns chromosome addition line with leaf rust resistance.

    Wanli Du

    Full Text Available The aim of this study was to characterize a Triticum aestivum-Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs disomic addition line 2-1-6-3. Individual line 2-1-6-3 plants were analyzed using cytological, genomic in situ hybridization (GISH, EST-SSR, and EST-STS techniques. The alien addition line 2-1-6-3 was shown to have two P. huashanica chromosomes, with a meiotic configuration of 2n = 44 = 22 II. We tested 55 EST-SSR and 336 EST-STS primer pairs that mapped onto seven different wheat chromosomes using DNA from parents and the P. huashanica addition line. One EST-SSR and nine EST-STS primer pairs indicated that the additional chromosome of P. huashanica belonged to homoeologous group 7, the diagnostic fragments of five EST-STS markers (BE404955, BE591127, BE637663, BF482781 and CD452422 were cloned, sequenced and compared. The results showed that the amplified polymorphic bands of P. huashanica and disomic addition line 2-1-6-3 shared 100% sequence identity, which was designated as the 7Ns disomic addition line. Disomic addition line 2-1-6-3 was evaluated to test the leaf rust resistance of adult stages in the field. We found that one pair of the 7Ns genome chromosomes carried new leaf rust resistance gene(s. Moreover, wheat line 2-1-6-3 had a superior numbers of florets and grains per spike, which were associated with the introgression of the paired P. huashanica chromosomes. These high levels of disease resistance and stable, excellent agronomic traits suggest that this line could be utilized as a novel donor in wheat breeding programs.

  9. Establishment, characterization and chemosensitivity of three mismatch repair deficient cell lines from sporadic and inherited colorectal carcinomas.

    Claudia Maletzki

    Full Text Available BACKGROUND: Colorectal cancer (CRC represents a morphologic and molecular heterogenic disease. This heterogeneity substantially impairs drug effectiveness and prognosis. The subtype of mismatch repair deficient (MMR-D CRCs, accounting for about 15% of all cases, shows particular differential responses up to resistance towards currently approved cytostatic drugs. Pre-clinical in vitro models representing molecular features of MMR-D tumors are thus mandatory for identifying biomarkers that finally help to predict responses towards new cytostatic drugs. Here, we describe the successful establishment and characterization of three patient-derived MMR-D cell lines (HROC24, HROC87, and HROC113 along with their corresponding xenografts. METHODOLOGY: MMR-D cell lines (HROC24, HROC87, and HROC113 were established from a total of ten clinicopathological well-defined MMR-D cases (120 CRC cases in total. Cells were comprehensively characterized by phenotype, morphology, growth kinetics, invasiveness, and molecular profile. Additionally, response to clinically relevant chemotherapeutics was examined in vitro and in vivo. PRINCIPAL FINDINGS: Two MMR-D lines showing CIMP-H derived from sporadic CRC (HROC24: K-ras(wt, B-raf(mut, HROC87: K-ras(wt, B-raf(mut, whereas the HROC113 cell line (K-ras(mut, B-raf(wt was HNPCC-associated. A diploid DNA-status could be verified by flow cytometry and SNP Array analysis. All cell lines were characterized as epithelial (EpCAM(+ tumor cells, showing surface tumor marker expression (CEACAM(+. MHC-class II was inducible by Interferon-γ stimulation. Growth kinetics as well as invasive potential was quite heterogeneous between individual lines. Besides, MMR-D cell lines exhibited distinct responsiveness towards chemotherapeutics, even when comparing in vitro and in vivo sensitivity. CONCLUSIONS: These newly established and well-characterized, low-passage MMR-D cell lines provide a useful tool for future investigations on the

  10. Isolation and characterization of a radiosensitive Chinese hamster ovary cell line

    Fuller, L.F.

    1987-01-01

    A x-ray sensitive Chinese hamster ovary cell line was isolated using a semi-automated procedure in which mutagenized CHO cells were allowed to form colonies on top of agar, x-irradiated, then photographed at two later times. Comparison of the photographs allowed the identification of colonies which displayed significant growth arrest. One of the colonies identified in this manner produced a stable, radiosensitive line. This cell line is normal in x-ray induced inhibition of DNA synthesis, and single- and double-strand break repair, and is moderately sensitive to ethyl methane sulfonate and UV light. The sensitive line performs only half as much x-ray-induced repair replication as the parental line and this deficiency is believed to be the primary cause of its radiosensitivity. The sensitive line produces significantly higher numbers of x-ray-induced chromosome and chromatid aberrations including chromatid aberrations following exposure during the G 1 phase of the cell cycle. The line is hypomutable compared to the parental line with x-ray exposure inducing only one-third as many 6-thioguanine resistant colonies

  11. Technology projects for characterization--monitoring of volatile organic compounds (VOCs)

    Junk, G.A.; Haas, W.J. Jr.

    1992-07-01

    One hundred thirty technology project titles related to the characterization of volatile organic compounds (VOCs) at an arid site are listed alphabetically by first contact person in a master compilation that includes phone numbers, addresses, keywords, and short descriptions. Separate tables are presented for 62 field-demonstrated, 36 laboratory-demonstrated, and 35 developing technology projects. The technology projects in each of these three categories are also prioritized in separate summary tables. Additional tables are presented for a number of other categorizations of the technology projects: In Situ; Fiberoptic; Mass Spectrometer; Optical Spectroscopy; Raman or SERS; Ion Mobility or Acoustic; Associated; and Commercial. Four lists of contact person names are provided so details concerning the projects that deal with sampling, and VOCs in gases, waters, and soils (sediments) can be obtained. Finally, seven wide-ranging conclusions based on observations and experiences during this work are presented.

  12. Technology projects for characterization--monitoring of volatile organic compounds (VOCs)

    Junk, G.A.; Haas, W.J. Jr.

    1992-07-01

    One hundred thirty technology project titles related to the characterization of volatile organic compounds (VOCs) at an arid site are listed alphabetically by first contact person in a master compilation that includes phone numbers, addresses, keywords, and short descriptions. Separate tables are presented for 62 field-demonstrated, 36 laboratory-demonstrated, and 35 developing technology projects. The technology projects in each of these three categories are also prioritized in separate summary tables. Additional tables are presented for a number of other categorizations of the technology projects: In Situ; Fiberoptic; Mass Spectrometer; Optical Spectroscopy; Raman or SERS; Ion Mobility or Acoustic; Associated; and Commercial. Four lists of contact person names are provided so details concerning the projects that deal with sampling, and VOCs in gases, waters, and soils (sediments) can be obtained. Finally, seven wide-ranging conclusions based on observations and experiences during this work are presented

  13. Discussion after implementation of possible ergonomics contributions in production lines automation project

    José Adriano Canton; Andrea Regina Martins Fontes; Isaías Torres

    2016-01-01

    This paper addresses the issue of work spaces designed based on Activity-Centered Ergonomics. The aim was to reflect on the role of Ergonomics in the design of productive situations in order to anticipate and mitigate the emergence of new constraints. This study was developed using a case study from the packaging industry, where an automated line was implemented to mitigate the injuries suffered as result of the manual system. This article was based on the results of an Ergonomic Work Analysi...

  14. Characterization of a Merkel Cell Polyomavirus-Positive Merkel Cell Carcinoma Cell Line CVG-1.

    Velásquez, Celestino; Amako, Yutaka; Harold, Alexis; Toptan, Tuna; Chang, Yuan; Shuda, Masahiro

    2018-01-01

    Merkel cell polyomavirus (MCV) plays a causal role in ∼80% of Merkel cell carcinomas (MCC). MCV is clonally integrated into the MCC tumor genome, which results in persistent expression of large T (LT) and small T (sT) antigen oncoproteins encoded by the early locus. In MCV-positive MCC tumors, LT is truncated by premature stop codons or deletions that lead to loss of the C-terminal origin binding (OBD) and helicase domains important for replication. The N-terminal Rb binding domain remains intact. MCV-positive cell lines derived from MCC explants have been valuable tools to study the molecular mechanism of MCV-induced Merkel cell carcinogenesis. Although all cell lines have integrated MCV and express truncated LT antigens, the molecular sizes of the LT proteins differ between cell lines. The copy number of integrated viral genome also varies across cell lines, leading to significantly different levels of viral protein expression. Nevertheless, these cell lines share phenotypic similarities in cell morphology, growth characteristics, and neuroendocrine marker expression. Several low-passage MCV-positive MCC cell lines have been established since the identification of MCV. We describe a new MCV-positive MCV cell line, CVG-1, with features distinct from previously reported cell lines. CVG-1 tumor cells grow in more discohesive clusters in loose round cell suspension, and individual cells show dramatic size heterogeneity. It is the first cell line to encode an MCV sT polymorphism resulting in a unique leucine (L) to proline (P) substitution mutation at amino acid 144. CVG-1 possesses a LT truncation pattern near identical to that of MKL-1 cells differing by the last two C-terminal amino acids and also shows an LT protein expression level similar to MKL-1. Viral T antigen knockdown reveals that, like other MCV-positive MCC cell lines, CVG-1 requires T antigen expression for cell proliferation.

  15. Visualization of Sensory Neurons and Their Projections in an Upper Motor Neuron Reporter Line.

    Genç, Barış; Lagrimas, Amiko Krisa Bunag; Kuru, Pınar; Hess, Robert; Tu, Michael William; Menichella, Daniela Maria; Miller, Richard J; Paller, Amy S; Özdinler, P Hande

    2015-01-01

    Visualization of peripheral nervous system axons and cell bodies is important to understand their development, target recognition, and integration into complex circuitries. Numerous studies have used protein gene product (PGP) 9.5 [a.k.a. ubiquitin carboxy-terminal hydrolase L1 (UCHL1)] expression as a marker to label sensory neurons and their axons. Enhanced green fluorescent protein (eGFP) expression, under the control of UCHL1 promoter, is stable and long lasting in the UCHL1-eGFP reporter line. In addition to the genetic labeling of corticospinal motor neurons in the motor cortex and degeneration-resistant spinal motor neurons in the spinal cord, here we report that neurons of the peripheral nervous system are also fluorescently labeled in the UCHL1-eGFP reporter line. eGFP expression is turned on at embryonic ages and lasts through adulthood, allowing detailed studies of cell bodies, axons and target innervation patterns of all sensory neurons in vivo. In addition, visualization of both the sensory and the motor neurons in the same animal offers many advantages. In this report, we used UCHL1-eGFP reporter line in two different disease paradigms: diabetes and motor neuron disease. eGFP expression in sensory axons helped determine changes in epidermal nerve fiber density in a high-fat diet induced diabetes model. Our findings corroborate previous studies, and suggest that more than five months is required for significant skin denervation. Crossing UCHL1-eGFP with hSOD1G93A mice generated hSOD1G93A-UeGFP reporter line of amyotrophic lateral sclerosis, and revealed sensory nervous system defects, especially towards disease end-stage. Our studies not only emphasize the complexity of the disease in ALS, but also reveal that UCHL1-eGFP reporter line would be a valuable tool to visualize and study various aspects of sensory nervous system development and degeneration in the context of numerous diseases.

  16. Characterization of a cultured human T-cell line with genetically altered ribonucleotide reductase activity. Model for immunodeficiency.

    Waddell, D; Ullman, B

    1983-04-10

    From human CCRF-CEM T-cells growing in continuous culture, we have selected, isolated, and characterized a clonal cell line, APHID-D2, with altered ribonucleotide reductase activity. In comparative growth rate experiments, the APHID-D2 cell line is less sensitive than the parental cell line to growth inhibition by deoxyadenosine in the presence of 10 microM erythro-9-(2-hydroxy-3-nonyl)adenine, an inhibitor of adenosine deaminase. The APHID-D2 cell line has elevated levels of all four dNTPs. The resistance of the APHID-D2 cell line to growth inhibition by deoxyadenosine and the abnormal dNTP levels can be explained by the fact that the APHID-D2 ribonucleotide reductase, unlike the parental ribonucleotide reductase, is not normally sensitive to inhibition by dATP. These results suggest that the allosteric site of ribonucleotide reductase which binds both dATP and ATP is altered in the APHID-D2 line. The isolation of a mutant clone of human T-cells which contains a ribonucleotide reductase that has lost its normal sensitivity to dATP and which is resistant to deoxyadenosine-mediated growth inhibition suggests that a primary pathogenic target of accumulated dATP in lymphocytes from patients with adenosine deaminase deficiency may be the cellular ribonucleotide reductase.

  17. Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor

    E.M. Lima

    2004-12-01

    Full Text Available Gastric cancer is the second most frequent type of neoplasia and also the second most important cause of death in the world. Virtually all the established cell lines of gastric neoplasia were developed in Asian countries, and western countries have contributed very little to this area. In the present study we describe the establishment of the cell line ACP01 and characterize it cytogenetically by means of in vitro immortalization. Cells were transformed from an intestinal-type gastric adenocarcinoma (T4N2M0 originating from a 48-year-old male patient. This is the first gastric adenocarcinoma cell line established in Brazil. The most powerful application of the cell line ACP01 is in the assessment of cytotoxicity. Solid tumor cell lines from different origins have been treated with several conventional and investigational anticancer drugs. The ACP01 cell line is triploid, grows as a single, non-organized layer, similar to fibroblasts, with focus formation, heterogeneous division, and a cell cycle of approximately 40 h. Chromosome 8 trisomy, present in 60% of the cells, was the most frequent cytogenetic alteration. These data lead us to propose a multifactorial triggering of gastric cancer which evolves over multiple stages involving progressive genetic changes and clonal expansion.

  18. On-Line Investment Analysis and Portfolio Management: Using Learning Outcome Statements To Design Projects.

    Pettijohn, James B.; Ragan, Gay A.; Ragan, Kent P.

    2003-01-01

    Describes an Internet-based project to familiarize students with online investment analysis and stock portfolio management. Outlines a process for writing learning outcomes that address three levels of cognition: knowledge/comprehension, application/analysis, and synthesis/evaluation. (SK)

  19. Functional design criteria radioactive liquid waste line replacement, Project W-087. Revision 3

    McVey, C.B.

    1994-01-01

    This document provides the functional design criteria for the 222-S Laboratory radioactive waste drain piping and transfer pipeline replacement. The project will replace the radioactive waste drain piping from the hot cells in 222-S to the 219-S Waste Handling Facility and provide a new waste transfer route from 219-S to the 244-S Catch Station in Tank Farms

  20. Managing for the triple bottom line in project-based learning

    Viljoen, NM

    2012-07-01

    Full Text Available Project-based learning is a popular engineering education approach that involves at least three stakeholders – students, faculty and an external client. But more often than not, one or more of these parties have to compromise their goals...

  1. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1995 quality program status report

    Bolivar, S.L.

    1996-07-01

    This status report summarizes the activities and accomplishments of the Los Alamos National Laboratory Yucca Mountain Site Characterization Project's (YMP's) quality assurance program for January 1 to September 30, 1995. The report includes major sections on program activities and trend analysis

  2. Purification and Characterization of Taq Polymerase: A 9-Week Biochemistry Laboratory Project for Undergraduate Students

    Bellin, Robert M.; Bruno, Mary K.; Farrow, Melissa A.

    2010-01-01

    We have developed a 9-week undergraduate laboratory series focused on the purification and characterization of "Thermus aquaticus" DNA polymerase (Taq). Our aim was to provide undergraduate biochemistry students with a full-semester continuing project simulating a research-like experience, while having each week's procedure focus on a single…

  3. Application of QA grading to Yucca Mountain Site Characterization Project items and activities

    Murthy, R.B.; Smith, S.C.

    1991-01-01

    Grading is the act of selecting the quality assurance (QA) measures necessary to develop and maintain confidence in the quality of an item or activity. The list of QA measures from which this selection is made are the 20 criteria of the Yucca Mountain Site Characterization Project Quality Assurance Requirements Document

  4. Los Alamos National Laboratory Yucca Mountain Site Characterization Project: 1991 quality program status report

    1992-07-01

    This status report summarizes the activities and accomplishments of the Los Alamos National Laboratory (Los Alamos) Yucca Mountain Site Characterization Project's (YMP) quality assurance program for calendar year 1991. The report is divided into three Sections: Program Activities, Verification Activities, and Trend Analysis

  5. A new cognitive rehabilitation programme for patients with multiple sclerosis: the 'MS-line! Project'.

    Gich, Jordi; Freixenet, Jordi; Garcia, Rafael; Vilanova, Joan Carles; Genís, David; Silva, Yolanda; Montalban, Xavier; Ramió-Torrentà, Lluís

    2015-09-01

    Cognitive rehabilitation is often delayed in multiple sclerosis (MS). To develop a free and specific cognitive rehabilitation programme for MS patients to be used from early stages that does not interfere with daily living activities. MS-line!, cognitive rehabilitation materials consisting of written, manipulative and computer-based materials with difficulty levels developed by a multidisciplinary team. Mathematical, problem-solving and word-based exercises were designed. Physical materials included spatial, coordination and reasoning games. Computer-based material included logic and reasoning, working memory and processing speed games. Cognitive rehabilitation exercises that are specific for MS patients have been successfully developed. © The Author(s), 2014.

  6. Establishment and characterization of a unique 1 μm diameter liver-derived progenitor cell line

    Aravalli, Rajagopal N.; Behnan Sahin, M.; Cressman, Erik N.K.; Steer, Clifford J.

    2010-01-01

    Liver-derived progenitor cells (LDPCs) are recently identified novel stem/progenitor cells from healthy, unmanipulated adult rat livers. They are distinct from other known liver stem/progenitor cells such as the oval cells. In this study, we have generated a LDPC cell line RA1 by overexpressing the simian virus 40 (SV40) large T antigen (TAg) in primary LDPCs. This cell line was propagated continuously for 55 passages in culture, after which it became senescent. Interestingly, following transformation with SV40 TAg, LDPCs decreased in size significantly and the propagating cells measured 1 μm in diameter. RA1 cells proliferated in vitro with a doubling time of 5-7 days, and expressed cell surface markers of LDPCs. In this report, we describe the characterization of this novel progenitor cell line that might serve as a valuable model to study liver cell functions and stem cell origin of liver cancers.

  7. Establishment and characterization of a unique 1 {mu}m diameter liver-derived progenitor cell line

    Aravalli, Rajagopal N., E-mail: arava001@umn.edu [Department of Radiology, University of Minnesota Medical School, Minneapolis, MN 55455 (United States); Behnan Sahin, M. [Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455 (United States); Cressman, Erik N.K. [Department of Radiology, University of Minnesota Medical School, Minneapolis, MN 55455 (United States); Steer, Clifford J., E-mail: steer001@umn.edu [Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455 (United States); Department of Genetics, Cell Biology, and Development, University of Minnesota Medical School, Minneapolis, MN 55455 (United States)

    2010-01-01

    Liver-derived progenitor cells (LDPCs) are recently identified novel stem/progenitor cells from healthy, unmanipulated adult rat livers. They are distinct from other known liver stem/progenitor cells such as the oval cells. In this study, we have generated a LDPC cell line RA1 by overexpressing the simian virus 40 (SV40) large T antigen (TAg) in primary LDPCs. This cell line was propagated continuously for 55 passages in culture, after which it became senescent. Interestingly, following transformation with SV40 TAg, LDPCs decreased in size significantly and the propagating cells measured 1 {mu}m in diameter. RA1 cells proliferated in vitro with a doubling time of 5-7 days, and expressed cell surface markers of LDPCs. In this report, we describe the characterization of this novel progenitor cell line that might serve as a valuable model to study liver cell functions and stem cell origin of liver cancers.

  8. Field line projections of 6300 AA auroral emissions into the outer magnetosphere

    Shepherd, M.M.

    1979-07-01

    An empirical magnetospheric model is employed to project auroral intensity boundaries into the magnetosphere. The auroral data are in the form of instantaneous maps of 6300AA emission, acquired with the ISIS-II spacecraft and correspond to fluxes of low energy electrons. These are specific to a particular universal time and date. The magnetospheric model used is a purely empirical one, designed by Mead and Fairfield (1975) from 44.76 x 10 6 magnetic measurements made by 4 IMP satellites. Their model includes the dipole tilt as a variable, and permits selection from four different disturbance levels, so is particularly suited to these data. In a general way, the auroral projections agree with what is expected, giving some confidence in this application of the model. But a number of features appear that were not predicted, and which should permit new insights into the relationship of specific auroral boundaries to the structure of the magnetosphere. (author)

  9. Characterization of seasonal reproduction in Virginia Tech Selection Line, St. Croix, and Suffolk ewes

    Jordan, Katherine Mead

    2008-01-01

    This dissertation research contained three studies. The first two studies were conducted to investigate the ability of ewes to rebreed while lactating during seasonal anestrus. Breeds studied included the Virginia Tech Out-of-season (OOS) Line, which is a wool line genetically selected to lamb in the fall, and the St. Croix, a hair breed of tropical origin thought to be lowly seasonal. When January-lambing ewes were exposed to rams while lactating in April, significantly more OOS than St. ...

  10. The Challenge of Interfacing the Primary Beam Lines for the AWAKE Project at CERN

    Bracco, C; Gschwendtner, E; Meddahi, M; Petrenko, A; Velotti, FM

    2014-01-01

    The Proton Driven Plasma Wakefield Acceleration Experiment (AWAKE) at CERN foresees the simultaneous operation of a proton, a laser and an electron beam. The first stage of the experiment will consist in proving the self-modulation, in the plasma, of a long proton bunch into micro-bunches. The success of this experiment requires an almost perfect concentricity of the proton and laser beam, over the full length of the plasma cell. The complexity of integrating the laser into the proton beam line and fulfilling the strict requirements in terms of pointing precision of the proton beam at the plasma cell are described. The second stage of the experiment foresees also the injection of electron bunches to probe the accelerating wakefields driven by the proton beam. Studies were performed to evaluate the possibility of injecting the electron beam parallel and with an offset to the beam axis. This option would imply that protons and electrons will have to share the last few meters of a common beam line. Issues and po...

  11. Characterization of stem-like cells in a new astroblastoma cell line

    Coban, Esra Aydemir; Kasikci, Ezgi [Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul (Turkey); Karatas, Omer Faruk [Molecular Biology and Genetics Department, Erzurum Technical University, Erzurum (Turkey); Suakar, Oznur; Kuskucu, Aysegul [Department of Medical Genetics, Yeditepe University Medical School and Yeditepe University Hospital, Istanbul (Turkey); Altunbek, Mine [Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul (Turkey); Türe, Uğur [Department of Neurosurgery, Yeditepe University School of Medicine, Istanbul (Turkey); Sahin, Fikrettin [Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul (Turkey); Bayrak, Omer Faruk, E-mail: ofbayrak@yeditepe.edu.tr [Department of Medical Genetics, Yeditepe University Medical School and Yeditepe University Hospital, Istanbul (Turkey)

    2017-03-15

    Cell lines established from tumors are the most commonly used models in cancer research, and their use in recent years has enabled a greater understanding of the biology of cancer and the means to develop effective treatment strategies. Astroblastomas are uncommon neuroepithelial tumors of glial origin, predominantly affecting young people, mainly teenagers and children, predominantly females. To date, only a single study has reported that astroblastomas contain a large number of neural stem-like cells, which had only a partial proliferation capacity and differentiation. Our objective was to establish an astroblastoma cell line to investigate the presence of astroblastic cells and cancer stem-like cells. The migratory and invasion abilities of the cells were quantified with invasion and migration assays and compared to a glioblastoma cell line. The presence of stem cells was detected with surface-marker analysis by using flow cytometry, and measuring the differentiation ability with a differentiation assay and the self-renewal capacity with a sphere-forming assay. These characteristics may determine whether this novel cell line is a model for astroblastomas that may have stem-cell characteristics. With this novel cell line, scientists can investigate the molecular pathways underlying astroblastomas and develop new therapeutic strategies for patients with these tumors. - Highlights: • An establishment of a novel astroblastoma cell line was proposed. • The presence of astroblastic cells and cancer stem-like cells was investigated. • The molecular pathways underlying astroblastomas may be investigated. • New therapeutic strategies for patients with astroblastoma may be developed.

  12. Engineered materials characterization report for the Yucca Mountain Site Characterization Project. Volume 2, Design data

    Konynenburg, R.A.; McCright, R.D.; Roy, A.K.; Jones, D.A.

    1995-08-01

    This is Volume 2 of the Engineered Materials Characterization Report which presents the design data for candidate materials needed in fabricating different components for both large and medium multi-purpose canister (MPC) disposal containers, waste packages for containing uncanistered spent fuel (UCF), and defense high-level waste (HLW) glass disposal containers. The UCF waste package consists of a disposal container with a basket therein. It is assumed that the waste packages will incorporate all-metallic multibarrier disposal containers to accommodate medium and large MPCs, ULCF, and HLW glass canisters. Unless otherwise specified, the disposal container designs incorporate an outer corrosion-allowance metal barrier over an inner corrosion-resistant metal barrier. The corrosion-allowance barrier, which will be thicker than the inner corrosion-resistant barrier, is designed to undergo corrosion-induced degradation at a very low rate, thus providing the inner barrier protection from the near-field environment for a prolonged service period

  13. An on-line communication system as an international catalysator for initiating storage projects

    Hennig, E.; Stephanblome, Th.

    1998-01-01

    The presented internet platform realizes an international contact stock of the companies and institutes being interested in storage technologies and cooperation and will take over the function of a catalysator for the planning of future storage use. In this regard the system creates an information and marketing device that will help, in the shape of an international, virtual exhibition hall, to find new markets, that are interesting for producers and suppliers of electrical energy storage technologies. In this virtual exhibition hall, need and offer regarding the electrical energy storage technologies are shown in order to support the main aim of the works regarding Annex IX, the starting of concrete projects. (author)

  14. Establishment, characterization, virus susceptibility and transfection of cell lines from cobia, Rachycentron canadum (L.), brain and fin.

    Cheng, T-C; Lai, Y-S; Lin, I-Y; Wu, C-P; Chang, S-L; Chen, T-I; Su, M-S

    2010-02-01

    Establishment and characterization of two cobia, Rachycentron canadum, cell lines derived from cobia brain (CB) and cobia fin (CF) are described. Caudal fin and brain from juvenile cobia were dissociated for 30 and 10 min, respectively, in phosphate-buffered saline containing 0.25% trypsin at 25 degrees C. The optimal culture condition for both dissociated cells (primary cell culture) was at 28 degrees C in Leibovitz-15 medium containing 10% foetal bovine serum. The cells have been sub-cultured at a ratio of 1:2 for more than 160 passages over a period of 3 years. Origin of the cultured cells was verified by comparison of their sequences of mitochondrial cytochrome oxidase subunit I genes (cox I) with the cox 1 sequence from cobia muscle tissue. The cell lines showed polyploidy. No mycoplasma contamination was detected. Susceptibility to grouper iridovirus was observed for the CB cell line but not the CF cell line. Both cell lines expressed green fluorescent protein after being transfected with green fluorescent reporter gene driven by the cytomegalovirus promoter.

  15. Generation and characterization of rat liver stem cell lines and their engraftment in a rat model of liver failure

    Kuijk, Ewart W.; Rasmussen, Shauna; Blokzijl, Francis; Huch, Meritxell; Gehart, Helmuth; Toonen, Pim; Begthel, Harry; Clevers, Hans; Geurts, Aron M.; Cuppen, Edwin

    2016-01-01

    The rat is an important model for liver regeneration. However, there is no in vitro culture system that can capture the massive proliferation that can be observed after partial hepatectomy in rats. We here describe the generation of rat liver stem cell lines. Rat liver stem cells, which grow as cystic organoids, were characterized by high expression of the stem cell marker Lgr5, by the expression of liver progenitor and duct markers, and by low expression of hepatocyte markers, oval cell markers, and stellate cell markers. Prolonged cultures of rat liver organoids depended on high levels of WNT-signalling and the inhibition of BMP-signaling. Upon transplantation of clonal lines to a Fah−/− Il2rg−/− rat model of liver failure, the rat liver stem cells engrafted into the host liver where they differentiated into areas with FAH and Albumin positive hepatocytes. Rat liver stem cell lines hold potential as consistent reliable cell sources for pharmacological, toxicological or metabolic studies. In addition, rat liver stem cell lines may contribute to the development of regenerative medicine in liver disease. To our knowledge, the here described liver stem cell lines represent the first organoid culture system in the rat. PMID:26915950

  16. Isolation and characterization of a new cell line from spontaneous mouse mammary tumour, MBL-6, for in vivo cancer studies

    Ladan Langroudi

    2017-12-01

    Full Text Available In search for treatments against breast cancer, cell lines are one of the basic resources, particularly as in vitro models. Additionally, animal models of cancer are used as the successive step in therapeutics research. In this regard, human breast cancer cell lines provide fundamental models in vitro. However, in vivo studies require immunodeficient mice, which lack the influence of other in vivo factors such as the native microenvironment and the immune system. There are few standard models to study the pathogenic mechanism at molecular level and cell signaling pathway of breast cancer. In this study, a new mouse breast cancer cell line, MBL-6, was successfully established and characterized from tissues of a spontaneous mammary tumor. The cell line had epithelial morphology, formed adherent monolayer, maintained continuously in vitro and was able to form new tumors when injected subcutaneously in syngeneic mice. The growth pattern and metastasis evaluations revealed a considerable in situ duration before invading distant organs. Real time polymerase chain reaction (PCR analysis showed the expression of ER-, PR- and Her-2 receptors. The chromosome analysis showed numerous chromosomal abnormalities. Aggressive tumorigenecity in tumorigenesis test and the IC50 to cyclophosphamide (CTX, celecoxib (CLX and cisplatin (CPN was also evaluated. The numerous tests performed on the new MBL-6 cell line suggest that it is in good quality and may be used in animal models of breast cancer studies.

  17. Large Area Projection Microstereolithography: Characterization and Optimization of 3D Printing Parameters

    Ng, Melissa R. [Ohlone College, Fremont, CA (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Moran, Bryan [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bekker, Logan [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Dudukovic, Nikola [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-08-12

    Large Area Projection Microstereolithography (LAPμSL) is a new technology that allows the additive manufacture of parts that have feature sizes spanning from centimeters to tens of microns. Knowing the accuracy of builds from a system like this is a crucial step in development. This project explored the capabilities of the second and newest LAPμSL system that was built by comparing the features of actual builds to the desired structures. The system was then characterized in order to achieve the best results. The photo polymeric resins that were used were Autodesk PR48 and HDDA. Build parameters for Autodesk PR48 were found that allowed the prints to progress while using the full capacity of the system to print quality parts in a relatively short amount of time. One of the larger prints in particular had a print time that was nearly eighteen times faster than it would have been had printed in the first LAPμSL system. The characterization of HDDA resin helped the understanding that the flux of the light projected into the resin also affected the quality of the builds, rather than just the dose of light given. Future work for this project includes exploring the use of other resins in the LAPμSL systems, exploring the use of Raman Spectroscopy to analyze builds, and completing the characterization of the LAPμSL system.

  18. In vitro characterization and inhibition of the CXCR4/CXCL12 chemokine axis in human uveal melanoma cell lines

    Antecka Emilia

    2007-11-01

    Full Text Available Abstract Purpose The CXCR4/CXCL12 chemokine axis may play a critical role in guiding CXCR4+ circulating malignant cells to organ specific locations that actively secrete its ligand CXCL12 (SDF-1 such as bone, brain, liver, and lungs. We sought to characterize the presence of the CXCR4/CXCL12 axis in five uveal melanoma (UM cell lines in vitro. The ability of TN14003, a synthetic peptide inhibitor that targets the CXCR4 receptor complex, to inhibit this axis was also assessed. Methods Immunocytochemistry was performed against CXCR4 to confirm expression of this chemokine receptor in all five UM cell lines. Flow cytometry was preformed to evaluate CXCR4 cell surface expression on all five UM cell lines. A proliferation assay was also used to test effects TN14003 would have on cellular proliferation. Inhibition of cellular migration by specifically inhibiting the CXCR4/CXCL12 axis with TN14003 was also investigated. The binding efficacy of TN14003 to the CXCR4 receptor was assessed through flow cytometric methods. Results The CXCR4 receptor was present on all five UM cell lines. All five cell lines expressed different relative levels of surface CXCR4. TN14003 did not affect the proliferation of the five cell lines (p > 0.05. All cell lines migrated towards the chemokine CXCL12 at a level greater than the negative control (p Conclusion Interfering with the CXCR4/CXCL12 axis, using TN14003 was shown to effectively down regulate UM cell migration in vitro. Knowing that UM expresses the CXCR4 receptor, these CXCR4+ cells may be less likely to colonize distant organs that secrete the CXCL12 ligand, if treated with an inhibitor that binds CXCR4. Further studies should be pursued in order to test TN14003 efficacy in vivo.

  19. Derivation and characterization of novel nonhuman primate embryonic stem cell lines from in vitro-fertilized baboon preimplantation embryos.

    Chang, Tien-Cheng; Liu, Ya-Guang; Eddy, Carlton A; Jacoby, Ethan S; Binkley, Peter A; Brzyski, Robert G; Schenken, Robert S

    2011-06-01

    The development of nonhuman primate (NHP) embryonic stem cell (ESC) models holds great promise for cell-mediated treatment of debilitating diseases and to address numerous unanswered questions regarding the therapeutic efficacy of ESCs while supplanting ethical considerations involved with human studies. Here we report successful establishment and characterization of 3 novel baboon (Papio cynocephalus) ESC lines from the inner cell mass of intracytoplasmic sperm injection-derived blastocysts. Embryos were cultured in an improved baboon embryo in vitro culture protocol. The inner cell mass of blastocyst was laser-dissected and plated on mouse embryonic fibroblast feeder cell monolayer in the NHP ESC culture medium. Three cell lines with characteristic ESC morphology have been cultured through an extended period (>14 months), with 2 male cell lines (UT-1 and -2) and 1 female cell line (UT-3) displaying normal baboon karyotypes. Reverse transcription-polymerase chain reaction analysis confirmed that all 3 lines express primate ESC pluripotency markers, including OCT-4, NANOG, SOX-2, TERT, TDGF, LEFTYA, and REX-1. All 3 lines demonstrated positive immunocytochemical staining for OCT-4, stage-specific embryonic antigen-3, stage-specific embryonic antigen-4, TRA-1-60, and TRA-1-81. Baboon ESCs injected into NOD/SCID mice formed teratomas with all 3 germ layers. In addition, embryoid body-like spherical structures were derived and initial outgrowth was observed when embedded into extracellular matrix Matrigel. The ESC lines established in this NHP model have the potential to extend our knowledge in the fields of developmental biology, regenerative medicine, and future applications, including preclinical safety assessment of in vivo stem cell therapy.

  20. In vitro culture and characterization of a mammary epithelial cell line from Chinese Holstein dairy cow.

    Han Hu

    Full Text Available BACKGROUND: The objective of this study was to establish a culture system and elucidate the unique characteristics of a bovine mammary epithelial cell line in vitro. METHODOLOGY: Mammary tissue from a three year old lactating dairy cow (ca. 100 d relative to parturition was used as a source of the epithelial cell line, which was cultured in collagen-coated tissue culture dishes. Fibroblasts and epithelial cells successively grew and extended from the culturing mammary tissue at the third day. Pure epithelial cells were obtained by passages culture. PRINCIPAL FINDINGS: The strong positive immunostaining to cytokeratin 18 suggested that the resulting cell line exhibited the specific character of epithelial cells. Epithelial cells cultured in the presence of 10% FBS, supraphysiologic concentrations of insulin, and hydrocortisone maintained a normal diploid chromosome modal number of 2n=60. Furthermore, they were capable of synthesizing beta-casein (CSN2, acetyl-CoA carboxylase-alpha (ACACA and butyrophilin (BTN1A1. An important finding was that frozen preservation in a mixture of 90% FBS and 10% DMSO did not influence the growth characteristics, chromosome number, or protein secretion of the isolated epithelial cell line. CONCLUSIONS: The obtained mammary epithelial cell line had normal morphology, growth characteristics, cytogenetic and secretory characteristics, thus, it might represent an useful tool for studying the function of Chinese Holstein dairy cows mammary epithelial cell (CMECs.

  1. Breeding and characterization of homokaryotic heteroplasmic male sterile lines in rice (oryza sativa)

    Mei, Q.; Zhou, X.; Liu, C.

    2011-01-01

    Twelve different Cytoplasmic Male-Sterile (CMS) lines were crossed with 18 rice varieties. From the hybrid with japonica rice Nongken 58, twelve homokaryotic-heteroplasmic male sterile lines were developed in B7F1 after successive back crossing and selection for stable male sterility and desirable agronomic traits such as flowering habit and high out crossing rate. The experimental results demonstrated that expression of the CMS factors were influenced by the corresponding nuclear genes. Three pollen abortion types, including the typical, the spherical and the stained abortion, were observed in the homokaryotic-heteroplasmic male sterile lines. Formation of the aborted pollen grains was influenced by the interaction among specific cytoplasmic and the corresponding nuclear genes. As the CMS carriers, these homokaryotic-heteroplasmic lines will have significant impact on the utilization of multiple types of CMS in hybrid rice breeding. What is more important is that these CMS lines are the invaluable materials for the investigation of the molecular mechanism of CMS formation in rice. (author)

  2. On-line fission products measurements during a PWR severe accident: the French DECA-PF project

    Ducros, G.; Allinei, P.G.; Roure, C. [CEA, DEN, F-13108 Saint-Paul-lez-Durance, (France); Rozel, C. [EDF SEPTEN, 12-14 Avenue Dutrievoz, F-69628, Villeurbanne, (France); Blanc De Lanaute, N. [CANBERRA, 1 rue des Herons, F-78182, Saint Quentin en Yvelines, (France); Musoyan, G. [AREVA, Tour AREVA, 1 place Jean Millier, F-92084 Paris La Defense Cedex, (France)

    2015-07-01

    Following the Fukushima accident, a lot of recommendations was drawn by international organizations (IAEA, OECD, NUGENIA network...) in order to improve the safety in such accidental conditions and mitigate their consequences. One of these recommendations was to improve the robustness of the instrumentation, which was dramatically lacking at Fukushima, as well as to better determine the Source Term involved in nuclear accident. The DECA-PF project (Diagnosis of a degraded reactor core through Fission Product measurements) was elaborated in this context and selected as one of 21 collaborative R and D projects in the field of nuclear safety and radioprotection, funded in May 2013 by the French National Research Agency. Over the months following the Fukushima accident, a CEA crisis team was held in order to analyze on-line the situation taking into account the data delivered by TEPCO and other organizations. Despite the difficulties encountered concerning the reliability of these data, the work performed showed the high capacity of Fission Products (FP) measurements to get a diagnosis relative to the status of the reactors and the spent fuel pools (SFP). Based on these FP measurements, it was possible to conclude that the main origin of the releases was coming from the cores and not from the SFP, in particular for SFP-4 which was of high concern, and that the degradation level of the reactors was very large, including probably an extensive core melting. To improve the reliability of this kind of diagnosis, the necessity to get such measurements as soon as possible after the accident and as near as possible from the reactor was stressed. In this way the present DECA-PF project intends to develop a new and innovative instrumentation taking into account the design of the French nuclear power plants on which sand bed filters have been implemented for severe accident management. Three complementary techniques, devoted to measure the FP release on-line, are being studied

  3. Characterization of spinal afferent neurons projecting to different chambers of the rat heart.

    Guić, Maja Marinović; Kosta, Vana; Aljinović, Jure; Sapunar, Damir; Grković, Ivica

    2010-01-29

    The pattern of distribution of spinal afferent neurons (among dorsal root ganglia-DRGs) that project to anatomically and functionally different chambers of the rat heart, as well as their morphological and neurochemical characteristics were investigated. Retrograde tracing using a patch loaded with Fast blue (FB) was applied to all four chambers of the rat heart and labeled cardiac spinal afferents were characterized by using three neurochemical markers. The majority of cardiac projecting neurons were found from T1 to T4 DRGs, whereas the peak was at T2 DRG. There was no difference in the total number of FB-labeled neurons located in ipsilateral and contralateral DRGs regardless of the chambers marked with the patch. However, significantly more FB-labeled neurons projected to the ventricles compared to the atria (859 vs. 715). The proportion of isolectin B(4) binding in FB-labeled neurons was equal among all neurons projecting to different heart chambers (2.4%). Neurofilament 200 positivity was found in greater proportions in DRG neurons projecting to the left side of the heart, whereas calretinin-immunoreactivity was mostly represented in neurons projecting to the left atrium. Spinal afferent neurons projecting to different chambers of the rat heart exhibit a variety of neurochemical phenotypes depending on binding capacity for isolectin B(4) and immunoreactivity for neurofilament 200 and calretinin, and thus represent important baseline data for future studies. (c) 2009 Elsevier Ireland Ltd. All rights reserved.

  4. Molecular characterization and functional analysis of pteridine reductase in wild-type and antimony-resistant Leishmania lines.

    de Souza Moreira, Douglas; Ferreira, Rafael Fernandes; Murta, Silvane M F

    2016-01-01

    Pteridine reductase (PTR1) is an NADPH-dependent reductase that participates in the salvage of pteridines, which are essential to maintain growth of Leishmania. In this study, we performed the molecular characterization of ptr1 gene in wild-type (WTS) and SbIII-resistant (SbR) lines from Leishmania guyanensis (Lg), Leishmania amazonensis (La), Leishmania braziliensis (Lb) and Leishmania infantum (Li), evaluating the chromosomal location, mRNA levels of the ptr1 gene and PTR1 protein expression. PFGE results showed that the ptr1 gene is located in a 797 kb chromosomal band in all Leishmania lines analyzed. Interestingly, an additional chromosomal band of 1070 kb was observed only in LbSbR line. Northern blot results showed that the levels of ptr1 mRNA are increased in the LgSbR, LaSbR and LbSbR lines. Western blot assays using the polyclonal anti-LmPTR1 antibody demonstrated that PTR1 protein is more expressed in the LgSbR, LaSbR and LbSbR lines compared to their respective WTS counterparts. Nevertheless, no difference in the level of mRNA and protein was observed between the LiWTS and LiSbR lines. Functional analysis of PTR1 enzyme was performed to determine whether the overexpression of ptr1 gene in the WTS L. braziliensis and L. infantum lines would change the SbIII-resistance phenotype of transfected parasites. Western blot results showed that the expression level of PTR1 protein was increased in the transfected parasites compared to the non-transfected ones. IC50 analysis revealed that the overexpression of ptr1 gene in the WTS L. braziliensis line increased 2-fold the SbIII-resistance phenotype compared to the non-transfected counterpart. Furthermore, the overexpression of ptr1 gene in the WTS L. infantum line did not change the SbIII-resistance phenotype. These results suggest that the PTR1 enzyme may be implicated in the SbIII-resistance phenotype in L. braziliensis line. Copyright © 2015 Elsevier Inc. All rights reserved.

  5. Formation and Characterization of Inkjet-Printed Nanosilver Lines on Plasma-Treated Glass Substrates

    Jae-Sung Kwon

    2018-02-01

    Full Text Available In this study, we investigated geometrical characteristics of the inkjet-printed lines with non-zero receding contact angle (CA on plasma-treated substrates in terms of various printing variables and analyzed the fluidic behavior and hydrodynamic instability involved in the line formation process. The printing variables included surface energy, droplet overlap ratio, printing frequency, a number of ink droplets, substrate temperature and printing procedures. For the study, a colloidal suspension containing 56 wt % silver nanoparticles in tetradecane solvent was used as a printing ink. It has electrical resistivity of 4.7 μΩ·cm. The substrates were obtained by performing a plasma enhanced chemical vapor deposition (PECVD process with C4F8 and O2 under various treatment conditions. As results of the experiments, the surface shape and pattern of the inkjet-printed Ag lines were dominantly influenced by the surface energy of the substrates, among the printing variables. Accordingly even when the receding CA was non-zero, bulging instability of the lines occurred forming separate circular patterns or regular bulges connected by ridges. It is a new finding of this study, which is completely different with the bulging instability of inkjet lines with zero receding CA specified by previous researches. The bulging instability decreased by increasing surface temperature of the substrates or employing interlacing procedure instead of continuous procedure for printing. The interlacing procedure also was advantageous to fabricate thick and narrow Ag lines with well-defined shape through overprinting on a hydrophobic substrate. These results will contribute greatly to not only the production of various printed electronics containing high-aspect-ratio structures but also the improvement of working performance of the devices.

  6. Characterization of environmental chemicals with potential for DNA damage using isogenic DNA repair-deficient chicken DT40 cell lines.

    Yamamoto, Kimiyo N; Hirota, Kouji; Kono, Koichi; Takeda, Shunichi; Sakamuru, Srilatha; Xia, Menghang; Huang, Ruili; Austin, Christopher P; Witt, Kristine L; Tice, Raymond R

    2011-08-01

    Included among the quantitative high throughput screens (qHTS) conducted in support of the US Tox21 program are those being evaluated for the detection of genotoxic compounds. One such screen is based on the induction of increased cytotoxicity in seven isogenic chicken DT40 cell lines deficient in DNA repair pathways compared to the parental DNA repair-proficient cell line. To characterize the utility of this approach for detecting genotoxic compounds and identifying the type(s) of DNA damage induced, we evaluated nine of 42 compounds identified as positive for differential cytotoxicity in qHTS (actinomycin D, adriamycin, alachlor, benzotrichloride, diglycidyl resorcinol ether, lovastatin, melphalan, trans-1,4-dichloro-2-butene, tris(2,3-epoxypropyl)isocyanurate) and one non-cytotoxic genotoxic compound (2-aminothiamine) for (1) clastogenicity in mutant and wild-type cells; (2) the comparative induction of γH2AX positive foci by melphalan; (3) the extent to which a 72-hr exposure duration increased assay sensitivity or specificity; (4) the use of 10 additional DT40 DNA repair-deficient cell lines to better analyze the type(s) of DNA damage induced; and (5) the involvement of reactive oxygen species in the induction of DNA damage. All compounds but lovastatin and 2-aminothiamine were more clastogenic in at least one DNA repair-deficient cell line than the wild-type cells. The differential responses across the various DNA repair-deficient cell lines provided information on the type(s) of DNA damage induced. The results demonstrate the utility of this DT40 screen for detecting genotoxic compounds, for characterizing the nature of the DNA damage, and potentially for analyzing mechanisms of mutagenesis. Copyright © 2011 Wiley-Liss, Inc.

  7. A different challenge: the directional drilled crossing for the Yacuiba - Rio Grande Gas Line Project - GASYRG

    Green, Wayne; Garcia, Francisco [Bolinter Ltda., Santa Cruz (Bolivia); Montano, Ruben [Transierra, Santa Cruz (Bolivia)

    2003-07-01

    The Rio Grande River's directional drilling, 2002 m. long and 25 m. deep was a great challenge for Transierra - Owner - as well as Laney - Bolinter - Contractor - to accomplish a feat yet to be done in the entire world. The dedication of the people involved showed their degree of professionalism that these companies have obtained and the determination in doing the job overcoming unforeseen obstacles and still being able to finish on time, mitigating environmental impacts and leaving a first class crossing. This document presents a description of the technical, logistic and construction factors that were involved in the project and which allowed to perform 7 directional drillings, including Rio Grande River, which during the pull got the last 60 m. of pipe stuck, being freed only after using a pneumatic hammer. (author)

  8. Seminoe-Kortes transmission line/substation consolidation project, Carbon County, Wyoming

    1990-07-01

    The existing switchyards at Western Area Power Administration's (WESTERN) Seminoe and Kortes facilities, located approximately 40 miles northeast of Rawlines, Carbon County, Wyoming, were constructed in 1939 and 1951, respectively. The circuit breakers at these facilities are beyond or approaching their service life and need to be replaced. In addition, the switchyards have poor access for maintenance and replacement of equipment, and their locations create potential for oil spills into the North Platte River. WESTERN is proposing to consolidate the switchyard facilities into one new substation to provide easier access, restore proper levels of system reliability, and decrease the potential for oil contamination of the river. This environmental assessment (EA) was prepared to evaluate the impacts of the proposed Seminoe-Kortes Consolidation Project. 57 refs., 12 figs., 8 tabs.

  9. Crowdsourcing Broad Absorption Line Properties and Other Features of Quasar Outflow Using Zooniverse Citizen Science Project Platform

    Crowe, Cassie; Lundgren, Britt; Grier, Catherine

    2018-01-01

    The Sloan Digital Sky Survey (SDSS) regularly publishes vast catalogs of quasars and other astronomical objects. Previously, the SDSS collaboration has used visual inspection to check quasar redshift validity and flag instances of broad absorption lines (BALs). This information helps researchers to easily single out the quasars with BAL properties and study their outflows and other intervening gas clouds. Due to the ever-growing number of new SDSS quasar observations, visual inspections are no longer possible using previous methods. Currently, BAL information is being determined entirely computationally, and the accuracy of that information is not precisely known. This project uses the Zooniverse citizen science platform to visually inspect quasar spectra for BAL properties, to check the accuracy of the current autonomous methods, and to flag multi-phase outflows and find candidates for in-falling gas into the quasar central engine. The layout and format of a Zooniverse project provides an easier way to inspect and record data on each spectrum and share the workload via crowdsourcing. Work done by the SDSS collaboration members is serving as a beta test for a public project upon the official release of the DR14 quasar catalog by SDSS.

  10. Characterization of rat lines with normotensive and hypertensive status using genomic fingerprinting

    Adarichev, V A; Korokhov, N P; Ostapchuk, Ia V; Dymshits, G M; Markel', A L; Ostaptchouk, Jana

    1996-01-01

    The properties of normotensive and hypertensive rat lines were investigated by the DNA fingerprinting method using a multilocus micro-satellite (CAC)5 probe. The HaeIII and HinfI restriction endonucleases were found to be the most informative enzymes in this case. The high genetic homogeneity of the

  11. Development and characterization of a bovine monocyte-derived macrophage cell line

    Monocytes circulate in the blood, and later differentiate into macrophages in the tissues. They are components of the innate arm of the immune response and are one of the first lines of defense again invading pathogens. However, they also serve as host cells for intracellular pathogens such as Mycob...

  12. CACAO: A project for a laboratory for the production and characterization of thin radioactive layers

    Bacri, C.O., E-mail: bacri@ipno.in2p3.f [Institut de Physique Nucleaire d' Orsay, 91406 Orsay Cedex, CNRS (UMR8608-IN2P3), Universite Paris-Sud (Paris XI) (France); Petitbon, V.; Pierre, S. [Institut de Physique Nucleaire d' Orsay, 91406 Orsay Cedex, CNRS (UMR8608-IN2P3), Universite Paris-Sud (Paris XI) (France)

    2010-02-11

    CACAO, Chimie des Actinides et Cibles radioActives a Orsay (actinide chemistry and radioactive targets at Orsay), is a project under construction that consists of the installation of a hot laboratory dedicated to the production and characterization of thin radioactive layers. The project aims to be a joint CNRS-CEA national laboratory to overcome difficulties related mainly to safety issues and to the lack of knowledge and potential manpower. The first goal is to fulfill, at least, the needs of the whole French community, and to be able to coordinate the different activities related to radioactive targets. For this purpose, itis important to be complementary to already existing international installations. Inside this framework, it will of course be possible to produce and/or characterize targets for other users.

  13. CACAO: A project for a laboratory for the production and characterization of thin radioactive layers

    Bacri, C. O.; Petitbon, V.; Pierre, S.; Cacao Group

    2010-02-01

    CACAO, Chimie des Actinides et Cibles radioActives à Orsay (actinide chemistry and radioactive targets at Orsay), is a project under construction that consists of the installation of a hot laboratory dedicated to the production and characterization of thin radioactive layers. The project aims to be a joint CNRS-CEA national laboratory to overcome difficulties related mainly to safety issues and to the lack of knowledge and potential manpower. The first goal is to fulfill, at least, the needs of the whole French community, and to be able to coordinate the different activities related to radioactive targets. For this purpose, itis important to be complementary to already existing international installations. Inside this framework, it will of course be possible to produce and/or characterize targets for other users.

  14. CACAO: A project for a laboratory for the production and characterization of thin radioactive layers

    Bacri, C.O.; Petitbon, V.; Pierre, S.

    2010-01-01

    CACAO, Chimie des Actinides et Cibles radioActives a Orsay (actinide chemistry and radioactive targets at Orsay), is a project under construction that consists of the installation of a hot laboratory dedicated to the production and characterization of thin radioactive layers. The project aims to be a joint CNRS-CEA national laboratory to overcome difficulties related mainly to safety issues and to the lack of knowledge and potential manpower. The first goal is to fulfill, at least, the needs of the whole French community, and to be able to coordinate the different activities related to radioactive targets. For this purpose, itis important to be complementary to already existing international installations. Inside this framework, it will of course be possible to produce and/or characterize targets for other users.

  15. Final report on the Background Soil Characterization Project at the Oak Ridge Reservation, Oak Ridge, Tennessee

    1993-10-01

    The Background Soil Characterization Project (BSCP) will provide background concentration levels of selected metals organic compounds, and radionuclides in soils from uncontaminated on-site areas at the Oak Ridge Reservation (ORR), and off-site in the western part of Roane County and the eastern part of Anderson County. The BSCP will establish a database, recommend how to use the data for contaminated site assessment, and provide estimates of the potential human health and environmental risks associated with the background level concentrations of potentially hazardous constituents. This volume contains the data from the Background Soil Characterization Project. When available, the following validation qualifiers are used in the appendixes. When validation qualifiers are not available, the corresponding contract laboratory data qualifiers appearing on the next page are used

  16. Development and Evaluation of an On-Line Educational Module for Volunteer Leaders on Bio-Security in Washington State 4-H Livestock Projects

    Stevenson, Jill L.; Moore, Dale A.; Newman, Jerry; Schmidt, Janet L.; Smith, Sarah M.; Smith, Jean; Kerr, Susan; Wallace, Michael; BoyEs, Pat

    2011-01-01

    An on-line module on disease prevention was created for 4-H volunteer leaders who work with livestock projects in Washington to better prepare them to teach youth about bio-security and its importance in 4-H livestock projects. Evaluation of the module and usage statistics since the module's debut were collected and evaluated. The module increases…

  17. Establishment and partial characterization of a cell line from burbot Lota lota maculosa: susceptibility to IHNV, IPNV and VHSV.

    Polinski, Mark P; Drennan, John D; Batts, William N; Ireland, Susan C; Cain, Kenneth D

    2010-05-18

    This study describes the development and partial characterization of a continuous fibroblastic-like cell line (BEF-1) developed from late stage embryos of North American burbot Lota lota maculosa. This cell line has been maintained for over 5 yr and 100 passages in vitro. Cells were cultured using Eagle's minimum essential medium with Earle's salts (MEM) supplemented with GlutaMAX, and 10% fetal bovine serum (FBS), pH 7.4. The addition of penicillin-streptomycin-neomycin (PSN) antibiotic mixture (0.05, 0.05, 0.1 mg m(-1), respectively) did not negatively influence cell replication; however, the antimycotic FungizoneTM (2.5 microg m(-1), amphotericin B) caused cell rounding and resulted in a severe decrease in cell proliferation. Optimal incubation temperature has been observed between 15 and 23 degrees C, and at these temperatures cultures are routinely passed using standard trypsinization methods every 5 to 7 d at a split ratio of 1:3 or 1:4. The cell line was susceptible to isolates of the M and U North American genotypes of infectious hematopoietic necrosis virus (IHNV), and to isolates of genotypes I, IVa, and IVb of viral hemorrhagic septicemia virus (VHSV). In contrast, the cell line was refractory to infection by 2 North American isolates of infectious pancreatic necrosis virus (IPNV) from serotypes A1 and A9. This cell line provides a new laboratory tool, will allow further investigation into viral diseases of burbot and possibly other species, and is the first immortalized cell line reported from a species in the Gadidae (cod) family.

  18. Establishment and partial characterization of a cell line from burbot Lota lota maculosa: susceptibility to IHNV, IPNV and VHSV.

    Batts, William N.; Polinski, Mark P.; Drennan, John D.; Ireland, Susan C.; Cain, Kenneth D.

    2010-01-01

    This study describes the development and partial characterization of a continuous fibroblastic-like cell line (BEF-1) developed from late stage embryos of North American burbot Lota lota maculosa. This cell line has been maintained for over 5 yr and 100 passages in vitro. Cells were cultured using Eagle’s minimum essential medium with Earle’s salts (MEM) supplemented with GlutaMAX™, and 10% fetal bovine serum (FBS), pH 7.4. The addition of penicillin-streptomycin-neomycin (PSN) antibiotic mixture (0.05, 0.05, 0.1 mg ml–1, respectively) did not negatively influence cell replication; however, the antimycotic Fungizone™ (2.5 µg ml–1, amphotericin B) caused cell rounding and resulted in a severe decrease in cell proliferation. Optimal incubation temperature has been observed between 15 and 23°C, and at these temperatures cultures are routinely passed using standard trypsinization methods every 5 to 7 d at a split ratio of 1:3 or 1:4. The cell line was susceptible to isolates of the M and U North American genotypes of infectious hematopoietic necrosis virus (IHNV), and to isolates of genotypes I, IVa, and IVb of viral hemorrhagic septicemia virus (VHSV). In contrast, the cell line was refractory to infection by 2 North American isolates of infectious pancreatic necrosis virus (IPNV) from serotypes A1 and A9. This cell line provides a new laboratory tool, will allow further investigation into viral diseases of burbot and possibly other species, and is the first immortalized cell line reported from a species in the Gadidae (cod) family.

  19. On Line Characterization of SiC Nanoparticles Produced by Laser Pyrolysis

    Wattieaux, G; Lecerf, P; Meyer, L; Boufendi, L; Leconte, Y; Sublemontier, O; Herlin, N; Asimakopoulou, A; Tsakis, A; Daskalos, M; Konstandopoulos, A G; Ouf, F X

    2011-01-01

    On Line Measurements campaigns have been carried out for the first time in IRAMIS/SPAM CEA's laser gas-phase pyrolysis nanoparticles production facilities. The produced aerosol is composed of Argon and/or Helium laden with SiC nanoparticles concentrated up to ∼14 mg/l. Different commercial apparatus were used for sampling, and characterisation of size, and morphology of the particles. A series of experiment is performed by IRSN with a DMS500 from Cambustion Ltd TM , which gives electrical mobility equivalent diameter distribution in real time. Particles are sampled with an ejector-diluter VKL-10 from Palas TM . On the same sampling line, APTL performed aerodynamic equivalent diameter mass distribution using a Nanomoudi from MSP Corp. TM and TEM post analysis, as well as post elemental analysis (EDS) after collection on TEM grid with a Thermophoretic Precipitator (TP). A previously developed model is used to obtain the aggregate morphology and primary particles sizes. In addition to these commercial or well-known techniques, a new patent-pending technique called RFPM (Radio Frequency Plasma Metrology developed by CILAS + GREMI in the frame of NANOCARA program) is tested for the first time off the lab. The principle is based on levitation of particles in plasma RF. The entire set up is a prototype, including the sampling line. Experiments showed that this technique is very promising for on line gas phase monitoring, even though future improvements are needed, especially on the direct injection of the sample in the measuring chamber. First results of size distribution on pyrolysis line were obtained: three modes of different geometric mean diameter D50 were measured. Further analysis of TEM micrographs gave insight on these three modes, they can be interpreted as primary particles, aggregated particles, and a third one which could be composed of silica oxide nanoparticles issued from the combustion of the remaining silane initiated by an air leak in the exhaust of

  20. Generation and characterization of human smooth muscle cell lines derived from atherosclerotic plaque.

    Bonin, L R; Madden, K; Shera, K; Ihle, J; Matthews, C; Aziz, S; Perez-Reyes, N; McDougall, J K; Conroy, S C

    1999-03-01

    The study of atherogenesis in humans has been restricted by the limited availability and brief in vitro life span of plaque smooth muscle cells (SMCs). We describe plaque SMC lines with extended life spans generated by the expression of the human papillomavirus (HPV)-16 E6 and E7 genes, which has been shown to extend the life span of normal adult human aortic SMCs. Resulting cell lines (pdSMC1A and 2) demonstrated at least 10-fold increases in life span; pdSMC1A became immortal. The SMC identity of both pdSMC lines was confirmed by SM22 mRNA expression. pdSMC2 were generally diploid but with various structural and numerical alterations; pdSMC1A demonstrated several chromosomal abnormalities, most commonly -Y, +7, -13, anomalies previously reported in both primary pdSMCs and atherosclerotic tissue. Confluent pdSMC2 appeared grossly similar to HPV-16 E6/E7-expressing normal adult aortic SMCs (AASMCs), exhibiting typical SMC morphology/growth patterns; pdSMC1A displayed irregular cell shape/organization with numerous mitotic figures. Dedifferentiation to a synthetic/proliferative phenotype has been hypothesized as a critical step in atherogenesis, because rat neonatal SMCs and adult intimal SMCs exhibit similar gene expression patterns. To confirm that our pdSMC lines likewise express this apparent plaque phenotype, osteopontin, platelet-derived growth factor B, and elastin mRNA levels were determined in pdSMC1A, pdSMC2, and AASMCs. However, no significant increases in osteopontin or platelet-derived growth factor B expression levels were observed in either pdSMC compared with AASMCs. pdSMC2 alone expressed high levels of elastin mRNA. Lower levels of SM22 mRNA in pdSMC1A suggested greater dedifferentiation and/or additional population doublings in pdSMC1A relative to pdSMC2. Both pdSMC lines (particularly 1A) demonstrated high message levels for matrix Gla protein, previously reported to be highly expressed by human neointimal SMCs in vitro. These results describe 2

  1. Environmental management project of the Patache power plant project and associated transmission line; Gestion ambiental proyecto central termoelectrica Patache y linea de transmision asociada

    Carreno Paredes, Cristian [Compania Electrica Tapaca (CELTA, S. A.), (Chile)

    1996-12-31

    This report makes a brief description of the services developed by INGENDESA of the projects executed in the area of engineering as well as in the area of environmental matters. In a second instance a relatively detailed presentation is made of the most important background of the Chilean project: {sup E}nvironmental Management of the Patache Power Plant and the Associated Transmission Line-Compania Electrica Tarapaca (CELTA, S.A.) Region I{sup .} The justification for the construction of the power plant and the environmental problems associated with the same are described [Espanol] En el presente informe se hace una breve presentacion de los servicios desarrollados por INGENDESA, de los proyectos elaborados tanto en el area de la ingenieria como en la tematica ambiental. En segunda instancia, se hace una presentacion relativamente detallada de los antecedentes mas importantes del proyecto chileno {sup G}estion Ambiental Central Patache y Linea de Transmision Asociada - Compania Electrica Tarapaca (CELTA, S.A.) I Region{sup .} Se describe la justificacion de la construccion de esta central termoelectrica y los problemas ambientales asociados a la misma

  2. Environmental management project of the Patache power plant project and associated transmission line; Gestion ambiental proyecto central termoelectrica Patache y linea de transmision asociada

    Carreno Paredes, Cristian [Compania Electrica Tapaca (CELTA, S. A.), (Chile)

    1997-12-31

    This report makes a brief description of the services developed by INGENDESA of the projects executed in the area of engineering as well as in the area of environmental matters. In a second instance a relatively detailed presentation is made of the most important background of the Chilean project: {sup E}nvironmental Management of the Patache Power Plant and the Associated Transmission Line-Compania Electrica Tarapaca (CELTA, S.A.) Region I{sup .} The justification for the construction of the power plant and the environmental problems associated with the same are described [Espanol] En el presente informe se hace una breve presentacion de los servicios desarrollados por INGENDESA, de los proyectos elaborados tanto en el area de la ingenieria como en la tematica ambiental. En segunda instancia, se hace una presentacion relativamente detallada de los antecedentes mas importantes del proyecto chileno {sup G}estion Ambiental Central Patache y Linea de Transmision Asociada - Compania Electrica Tarapaca (CELTA, S.A.) I Region{sup .} Se describe la justificacion de la construccion de esta central termoelectrica y los problemas ambientales asociados a la misma

  3. Characterization of human lymphoid cell lines GM9947 and GM9948 as intra- and interlaboratory reference standards for DNA typing

    Fregeau, C.J.; Elliott, J.C.; Fourney, R.M. [RCMP Central Forensic Laboratory, Ottawa, Ontario (Canada)] [and others

    1995-07-20

    The incorporation of reference DNA is crucial to the validation of any DNA typing protocol. Currently, reference DNA standards are restricted to molecular size DNA ladders and/or tumor cell line DNA. Either of these, however, presents some limitations. We have rigorously characterized two Epstein-Barr virus (EBV)-immortalized human lymphoid cell lines-GM9947 (female) and GM9948 (male)-to determine their suitability as alternative in-line standards for three widely employed allele profiling strategies. Twenty-one highly polymorphic VNTR-based allelic systems (7 RFLPs, 2 AmpFLPs, and 12 STRs) distributed over 12 chromosomes were scrutinized along with 3 gender-based discriminatory systems. The genetic stability of each locus was confirmed over a period of 225 in vitro population doublings. Allele size estimates and degree of informativeness for each of the 21 VNTR systems were compiled. The reproducibility of allele scoring by traditional RFLP analyses, using both cell lines as reference standards, was also verified by an interlaboratory validation study involving 13 analysts from two geographically distinct forensic laboratories. Taken together, our data indicate that GM9947 and GM9948 genomic DNAs could be adopted as reliable reference standards for DNA typing. 82 refs., 3 figs., 8 tabs.

  4. SMART 3D SUBSURFACE CONTAMINANT CHARACTERIZATION AT THE BGRR DEC OMMISSIONING PROJECT

    HEISER, J.; KALB, P.; SULLIVAN, T.; MILIAN, L.

    2002-01-01

    The Brookhaven Graphite Research Reactor is currently on an accelerated decommissioning schedule with a completion date projected for 2005. The accelerated schedule combines characterization with removal actions for the various systems and structures. A major project issue involves characterization of the soils beneath contaminated Below Grade Ducts (BGD), the main air ducts connecting the exhaust plenums with the Fan House. The air plenums experienced water intrusion during BGRR operations and after shutdown. The water intrusions were attributed to rainwater leaks into degraded parts of the system, and to internal cooling water system leaks. If the characterization could provide enough information to show that soil contamination surrounding the BGD is either below cleanup guidelines or is very localized and can be ''surgically removed'' at a reasonable cost, the ducts may be decontaminated and left in place. This will provide significant savings compared to breaking up the 170-ft. long concrete duct, shipping the projected 9,000 m 3 of waste off-site and disposing of it in an approved site

  5. GENISES: A GIS Database for the Yucca Mountain Site Characterization Project

    Beckett, J.

    1991-01-01

    This paper provides a general description of the Geographic Nodal Information Study and Evaluation System (GENISES) database design. The GENISES database is the Geographic Information System (GIS) component of the Yucca Mountain Site Characterization Project Technical Database (TDB). The GENISES database has been developed and is maintained by EG ampersand G Energy Measurements, Inc., Las Vegas, NV (EG ampersand G/EM). As part of the Yucca Mountain Project (YMP) Site Characterization Technical Data Management System, GENISES provides a repository for geographically oriented technical data. The primary objective of the GENISES database is to support the Yucca Mountain Site Characterization Project with an effective tool for describing, analyzing, and archiving geo-referenced data. The database design provides the maximum efficiency in input/output, data analysis, data management and information display. This paper provides the systematic approach or plan for the GENISES database design and operation. The paper also discusses the techniques used for data normalization or the decomposition of complex data structures as they apply to GIS database. ARC/INFO and INGRES files are linked or joined by establishing ''relate'' fields through the common attribute names. Thus, through these keys, ARC can allow access to normalized INGRES files greatly reducing redundancy and the size of the database

  6. Study of ultrasonic characterization and propagation in austenitic welds: The MOSAICS project

    Chassignole, Bertrand, E-mail: bertrand.chassignole@edf.fr [EDF R and D, MMC department, Les Renardières, 77818 Moret sur Loing (France); Recolin, Patrick, E-mail: patrick.recolin@dcnsgroup.com [DCNS CESMAN, 44620 La montagne (France); Leymarie, Nicolas, E-mail: nicolas.leymarie@cea.fr [CEA LIST, 91191 Gif-sur-Yvette (France); Gueudré, Cécile, E-mail: cecile.gueudre@univ-amu.fr [LMA, Aix Marseille Université, CNRS, UPR 7051, F-13402 Marseille Cedex 20 (France); Guy, Philippe, E-mail: philippe.guy@insa-lyon.fr [INSA Lyon, LVA laboratory, 69621 Villeurbanne (France); Elbaz, Deborah, E-mail: deborah.elbaz@extende.com [Extende, 91400 Orsay (France)

    2015-03-31

    Regulatory requirements enforce a volumetric inspection of welded components of nuclear equipments. However, the multi-pass austenitic welds are characterized by anisotropic and heterogeneous structures which lead to numerous disturbances of the ultrasonic beam. The MOSAICS project supported by the ANR (French National Research Agency) aims at matching various approaches to improve the prediction of the ultrasonic testing in those welds. The first stage consists in characterizing the weld structure (determination of the columnar grain orientation and measurements of elastic constants and attenuation coefficients). The techniques of characterization provide input data for the modeling codes developed in another task of the project. For example, a 3D version of the finite elements code ATHENA is developed by EDF R and D to take into account anisotropic texture in any direction. Semi-analytical models included in CIVA software are also improved to better predict the ultrasonic propagation in highly anisotropic and heterogeneous structures. The last stage deals with modeling codes validation based on experimental inspections on representative mock-ups containing calibrated defects. The objective of this paper is to give an overview of the MOSAICS project and to present specific results illustrating the various tasks.

  7. Characterization of bond line discontinuities in a high-Mn TWIP steel pipe welded by HF-ERW

    Park, Gitae; Kim, Bongyoon; Kang, Yongjoon [Division of Materials Science and Engineering, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul 04763 (Korea, Republic of); Kang, Heewoong [RD Team, Husteel, 131 Bugokgongdan-ro, Songak-eup, Dangjin-si, Chungnam 31721 (Korea, Republic of); Lee, Changhee, E-mail: chlee@hanyang.ac.kr [Division of Materials Science and Engineering, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul 04763 (Korea, Republic of)

    2016-08-15

    In this work, the microstructure and defects in a high-frequency electrical resistance welded (HF-ERW) pipe of high-Mn twinning-induced plasticity (TWIP) steel were characterized. The microstructure of the base metal and the bond line were examined using both optical microscopy and scanning electron microscopy. The features of the bond line were similar to those of conventional steel. Simultaneously, the circumferential ductility was evaluated via a flaring test. It was concluded that the deterioration of the circumferential ductility in a high-Mn TWIP steel pipe was caused by irregular shaped oxide defects and a penetrator that had been formed during welding. Specifically, the penetrator, which is composed of MnO and Mn{sub 2}SiO{sub 4}, was found to be the most influential on the circumferential ductility of the welded pipe. The penetrator was analyzed using both an electron probe micro analyzer and transmission electron microscopy, and the formation sequence of the penetrator was evaluated. - Highlights: •This study focused on applying the HF-ERW process to the seam welding of expandable pipe using TWIP steels. •For improvement of the circumferential ductility, deterioration factors were characterized. •Penetrator which would mainly deteriorate the circumferential ductility consisted of round MnO and Mn{sub 2}SiO{sub 4}. •Metallurgical evidence of existing theory regarding the mechanism of defect formation during the HF-ERW was characterized.

  8. The hybrid reactor project based on the straight field line mirror concept

    Ågren, O.; Noack, K.; Moiseenko, V. E.; Hagnestål, A.; Källne, J.; Anglart, H.

    2012-01-01

    The straight field line mirror (SFLM) concept is aiming towards a steady-state compact fusion neutron source. Besides the possibility for steady state operation for a year or more, the geometry is chosen to avoid high loads on materials and plasma facing components. A comparatively small fusion hybrid device with “semi-poor” plasma confinement (with a low fusion Q factor) may be developed for industrial transmutation and energy production from spent nuclear fuel. This opportunity arises from a large fission to fusion energy multiplication ratio, Q r = P fis /P fus >>1. The upper bound on Q r is primarily determined by geometry and reactor safety. For the SFLM, the upper bound is Q r ≈150, corresponding to a neutron multiplicity of k eff =0.97. Power production in a mirror hybrid is predicted for a substantially lower electron temperature than the requirement T e ≈10 keV for a fusion reactor. Power production in the SFLM seems possible with Q≈0.15, which is 10 times lower than typically anticipated for hybrids (and 100 times smaller than required for a fusion reactor). This relaxes plasma confinement demands, and broadens the range for use of plasmas with supra-thermal ions in hybrid reactors. The SFLM concept is based on a mirror machine stabilized by qudrupolar magnetic fields and large expander tanks beyond the confinement region. The purpose of the expander tanks is to distribute axial plasma loss flow over a sufficiently large area so that the receiving plates can withstand the heat. Plasma stability is not relying on a plasma flow into the expander regions. With a suppressed plasma flow into the expander tanks, a possibility arise for higher electron temperature. A brief presentation will be given on basic theory for the SFLM with plasma stability and electron temperature issues, RF heating computations with sloshing ion formation, neutron transport computations with reactor safety margins and material load estimates, magnetic coil designs as well as

  9. The hybrid reactor project based on the straight field line mirror concept

    Ågren, O.; Noack, K.; Moiseenko, V. E.; Hagnestâl, A.; Källne, J.; Anglart, H.

    2012-06-01

    The straight field line mirror (SFLM) concept is aiming towards a steady-state compact fusion neutron source. Besides the possibility for steady state operation for a year or more, the geometry is chosen to avoid high loads on materials and plasma facing components. A comparatively small fusion hybrid device with "semi-poor" plasma confinement (with a low fusion Q factor) may be developed for industrial transmutation and energy production from spent nuclear fuel. This opportunity arises from a large fission to fusion energy multiplication ratio, Qr = Pfis/Pfus>>1. The upper bound on Qr is primarily determined by geometry and reactor safety. For the SFLM, the upper bound is Qr≈150, corresponding to a neutron multiplicity of keff=0.97. Power production in a mirror hybrid is predicted for a substantially lower electron temperature than the requirement Te≈10 keV for a fusion reactor. Power production in the SFLM seems possible with Q≈0.15, which is 10 times lower than typically anticipated for hybrids (and 100 times smaller than required for a fusion reactor). This relaxes plasma confinement demands, and broadens the range for use of plasmas with supra-thermal ions in hybrid reactors. The SFLM concept is based on a mirror machine stabilized by qudrupolar magnetic fields and large expander tanks beyond the confinement region. The purpose of the expander tanks is to distribute axial plasma loss flow over a sufficiently large area so that the receiving plates can withstand the heat. Plasma stability is not relying on a plasma flow into the expander regions. With a suppressed plasma flow into the expander tanks, a possibility arise for higher electron temperature. A brief presentation will be given on basic theory for the SFLM with plasma stability and electron temperature issues, RF heating computations with sloshing ion formation, neutron transport computations with reactor safety margins and material load estimates, magnetic coil designs as well as a discussion on

  10. TEM characterization of invariant line interfaces and structural ledges in a Mo-Si alloy

    Xiao, S.Q.; Dahmen, U.; Maloy, S.A.; Heuer, A.H.

    1995-06-01

    Two distinct lath morphologies of Mo 5 Si 3 precipitates observed in MoSi 2 differ in their cross-sectional shape and lattice orientation. Type I laths exhibit a rectangular cross section, with interfaces parallel to low-index planes, while Type II laths are parallelogram-shaped, with their major interface at 13 degree to the Type I precipitate. The corresponding orientation relationships differ by a 1.8 degree rotation around the lath axis. In this study, the difference between the two characteristic morphologies and orientation relationships is shown to be the formation of an invariant line strain for Type II precipitates. On an atomic scale, both interfaces have a terrace and ledge structure but differ in the stacking sequence of interfacial ledges associated with partial dislocations. The structural unit model and the invariant line model predict identical interface geometries which agree closely with the observations

  11. Comprehensive characterization of genomic instability in pluripotent stem cells and their derived neuroprogenitor cell lines

    Nestor Luis Lopez Corrales

    2012-12-01

    Full Text Available The genomic integrity of two human pluripotent stem cells and their derived neuroprogenitor cell lines was studied, applying a combination of high-resolution genetic methodologies. The usefulness of combining array-comparative genomic hybridization (aCGH and multiplex fluorescence in situ hybridization (M-FISH techniques should be delineated to exclude/detect a maximum of possible genomic structural aberrations. Interestingly, in parts different genomic imbalances at chromosomal and subchromosomal levels were detected in pluripotent stem cells and their derivatives. Some of the copy number variations were inherited from the original cell line, whereas other modifications were presumably acquired during the differentiation and manipulation procedures. These results underline the necessity to study both pluripotent stem cells and their differentiated progeny by as many approaches as possible in order to assess their genomic stability before using them in clinical therapies.

  12. Combining On-Line Characterization Tools with Modern Software Environments for Optimal Operation of Polymerization Processes

    Navid Ghadipasha

    2016-02-01

    Full Text Available This paper discusses the initial steps towards the formulation and implementation of a generic and flexible model centric framework for integrated simulation, estimation, optimization and feedback control of polymerization processes. For the first time it combines the powerful capabilities of the automatic continuous on-line monitoring of polymerization system (ACOMP, with a modern simulation, estimation and optimization software environment towards an integrated scheme for the optimal operation of polymeric processes. An initial validation of the framework was performed for modelling and optimization using literature data, illustrating the flexibility of the method to apply under different systems and conditions. Subsequently, off-line capabilities of the system were fully tested experimentally for model validations, parameter estimation and process optimization using ACOMP data. Experimental results are provided for free radical solution polymerization of methyl methacrylate.

  13. Haploids in Conifer Species: Characterization and Chromosomal Integrity of a Maritime Pine Cell Line

    José Antonio Cabezas

    2016-11-01

    Full Text Available Haploids are a valuable tool for genomic studies in higher plants, especially those with huge genome size and long juvenile periods, such as conifers. In these species, megagametophyte cultures have been widely used to obtain haploid callus and somatic embryogenic lines. One of the main problems associated with tissue culture is the potential genetic instability of the regenerants. Because of this, chromosomal stability of the callus and/or somatic embryos should also be assessed. To this end, chromosome counting, flow cytometry and genotyping using microsatellites have been reported. Here, we present an overview of the work done in conifers, with special emphasis on the production of a haploid cell line in maritime pine (Pinus pinaster L. and the use of a set of molecular markers, which includes Single Nucleotide Polymorphisms (SNPs and microsatellites or Single Sequence Repeats (SSRs, to validate chromosomal integrity confirming the presence of all chromosomic arms.

  14. Generation and characterization of PDGFRα-GFPCreERT2 knock-In mouse line.

    Miwa, Hiroyuki; Era, Takumi

    2015-05-01

    Platelet-derived growth factor (PDGF) and its receptor play an important role in embryogenesis. PDGF receptor α (PDGFRα) is expressed specifically in the embryonic day 7.5 (E7.5) mesoderm and in the E9.5 neural crest among other tissues. PDGFRα-expressing cells and their descendants are involved in the formation of various tissues. To trace PDGFRα-expressing cells in vivo, we generated a knock-in mouse line that expressed a fusion protein of green fluorescent protein (GFP), Cre recombinase (Cre), and mutated estrogen receptor ligand-binding domain (ERT2) under the control of the PDGFRα promoter. In these mice, Cre activity in PDGFRα-expressing cells could be induced by tamoxifen treatment. Taken together, our results suggest that the knock-in mouse line generated here could be useful for studying PDGFRα-expressing cells and their descendants in vivo at various stages of development. © 2015 Wiley Periodicals, Inc.

  15. Summary of field operations Technical Area I well PGS-1. Site-Wide Hydrogeologic Characterization Project

    Fritts, J.E.; McCord, J.P.

    1995-02-01

    The Environmental Restoration (ER) Project at Sandia National Laboratories, New Mexico is managing the project to assess and, when necessary, to remediate sites contaminated by the lab operations. Within the ER project, the site-wide hydrogeologic characterization task is responsible for the area-wide hydrogeologic investigation. The purpose of this task is to reduce the uncertainty about the rate and direction of groundwater flow beneath the area and across its boundaries. This specific report deals with the installation of PGS-1 monitoring well which provides information on the lithology and hydrology of the aquifer in the northern area of the Kirtland Air Force Base. The report provides information on the well design; surface geology; stratigraphy; structure; drilling, completion, and development techniques; and borehole geophysics information

  16. Identification and characterization of proteins involved in nuclear organization using Drosophila GFP protein trap lines.

    Margaret Rohrbaugh

    Full Text Available Strains from a collection of Drosophila GFP protein trap lines express GFP in the normal tissues where the endogenous protein is present. This collection can be used to screen for proteins distributed in the nucleus in a non-uniform pattern.We analyzed four lines that show peripheral or punctate nuclear staining. One of these lines affects an uncharacterized gene named CG11138. The CG11138 protein shows a punctate distribution in the nuclear periphery similar to that of Drosophila insulator proteins but does not co-localize with known insulators. Interestingly, mutations in Lamin proteins result in alterations in CG11138 localization, suggesting that this protein may be a novel component of the nuclear lamina. A second line affects the Decondensation factor 31 (Df31 gene, which encodes a protein with a unique nuclear distribution that appears to segment the nucleus into four different compartments. The X-chromosome of males is confined to one of these compartments. We also find that Drosophila Nucleoplasmin (dNlp is present in regions of active transcription. Heat shock leads to loss of dNlp from previously transcribed regions of polytene chromosome without redistribution to the heat shock genes. Analysis of Stonewall (Stwl, a protein previously found to be necessary for the maintenance of germline stem cells, shows that Stwl is present in a punctate pattern in the nucleus that partially overlaps with that of known insulator proteins. Finally we show that Stwl, dNlp, and Df31 form part of a highly interactive network. The properties of other components of this network may help understand the role of these proteins in nuclear biology.These results establish screening of GFP protein trap alleles as a strategy to identify factors with novel cellular functions. Information gained from the analysis of CG11138 Stwl, dNlp, and Df31 sets the stage for future studies of these proteins.

  17. Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair

    Miyaji E.N.

    2000-01-01

    Full Text Available Using a positive selection system for isolating DNA replication and repair related mutants, we isolated a clone from a rat kangaroo cell line (PtK2 that has increased sensitivity to UV light. Characterization of this clone indicated normal post-replication repair after UV irradiation, and normal removal rates of cyclobutane pyrimidine dimers and pyrimidine(6-4pyrimidone photoproducts by excision repair. However, this cell line has decreased ability to make early incisions on damaged DNA, possibly indicating a defect in preferential repair of actively transcribed genes, and a slower cell proliferation rate, including a longer S-phase. This phenotype reinforces the present notion that control of key mechanisms in cell metabolism, such as cell cycle control, repair, transcription and cell death, can be linked.

  18. Environmental Restoration Program pollution prevention checklist guide for the facility characterization project phase

    1993-09-01

    A facility characterization (FC) is conducted to determine the nature and extent contamination at a potential hazardous facility waste site. The information gathered during an FC includes (1) data on the volume and chemical nature of the waste, (2) information on the extent of contamination and the migration potential of the contaminants, (3) preliminary information on evaluation of alternative concepts that can or cannot be considered, and (4)supportive technical and cost data. For the purposes of identification, the following operational phases will be used for definition for this phase of the decommissioning and decontamination process (1) facility characterization before clean up, (2) characterization during clean up, (3) characterization of waste materials, and (4) site characterization after clean up. A key consideration in this process is the prevention of any waste to be generated from these characterization activities. The purpose of this checklist guide is to assist users with incorporating pollution prevention/waste minimization (PP/WM) in all FC phase projects of the Environmental Restoration (ER) Program. This guide will help users document PP/WM activities for technology transfer and reporting requirements. Automated computer screens will be created from the checklist data to assist users with implementing and evaluating waste reduction

  19. Establishment and Characterization of New Canine and Feline Osteosarcoma Primary Cell Lines

    Florian R. L. Meyer

    2016-06-01

    Full Text Available Osteosarcomas are the most abundant form of bone malignancies in multiple species. Canine osteosarcomas are considered a valuable model for human osteosarcomas because of their similar features. Feline osteosarcomas, on the other hand, are rarely studied but have interesting characteristics, such as a better survival prognosis than dogs or humans, and less likelihood of metastasis. To enable experimental approaches to study these differences we have established five new canine osteosarcoma cell lines out of three tumors, COS_1186h, COS_1186w, COS_1189, and COS_1220, one osteosarcoma-derived lung metastasis, COS_1033, and two new feline osteosarcoma cell lines, FOS_1077 and FOS_1140. Their osteogenic and neoplastic origin, as well as their potential to produce calcified structures, was determined by the markers osteocalcin, osteonectin, tissue unspecific alkaline phosphatase, p53, cytokeratin, vimentin, and alizarin red. The newly developed cell lines retained most of their markers in vitro but only spontaneously formed spheroids produced by COS_1189 showed calcification in vitro.

  20. Genetic characterization of Bombyx mori (Lepidoptera: Bombycidae) breeding and hybrid lines with different geographic origins.

    Furdui, Emilia M; Mărghitaş, Liviu A; Dezmirean, Daniel S; Paşca, Ioan; Pop, Iulia F; Erler, Silvio; Schlüns, Ellen A

    2014-01-01

    The domesticated silkworm Bombyx mori L. comprises a large number of geographical breeds and hybrid lines. Knowing the genetic structure of those may provide information to improve the conservation of commercial lines by estimating inbreeding over generations and the consequences of excessive use of those lineages. Here, we analyzed the genetic diversity of seven breeds and eight hybrid lines from Eastern Europe and Asia using highly polymorphic microsatellites markers to determine its genetical impact on their use in global breeding programs. No consistent pattern of deviation from Hardy-Weinberg equilibrium was found for most breed and hybrids; and the absence of a linkage disequilibrium also suggests that the strains are in equilibrium. A principal coordinate analysis revealed a clear separation of two silkworm breeds from the rest: one (IBV) originated from India and the other one (RG90) from Romania/Japan. The tendency of the other breeds from different geographic origins to cluster together in a general mix might be due to similar selection pressures (climate and anthropogenic factors) in different geographic locations. Phylogenetic analyses grouped the different silkworm breeds but not the hybrids according to their geographic origin and confirmed the pattern found in the principal coordinate analysis. © The Author 2014. Published by Oxford University Press on behalf of the Entomological Society of America.

  1. Grain characterization and milling behaviour of near-isogenic lines differing by hardness.

    Greffeuille, V; Abecassis, J; Rousset, M; Oury, F-X; Faye, A; L'Helgouac'h, C Bar; Lullien-Pellerin, V

    2006-12-01

    Wheat grain hardness is a major factor affecting the milling behaviour and end-product quality although its exact structural and biochemical basis is still not understood. This study describes the development of new near-isogenic lines selected on hardness. Hard and soft sister lines were characterised by near infrared reflectance (NIR) and particle size index (PSI) hardness index, grain protein content, thousand kernel weight and vitreousness. The milling behaviour of these wheat lines was evaluated on an instrumented micromill which also measures the grinding energy and flour particle size distribution was investigated by laser diffraction. Endosperm mechanical properties were measured using compression tests. Results pointed out the respective effect of hardness and vitreousness on those characteristics. Hardness was shown to influence both the mode of fracture and the mechanical properties of the whole grain and endosperm. Thus, this parameter also acts on milling behaviour. On the other hand, vitreousness was found to mainly play a role on the energy required to break the grain. This study allows us to distinguish between consequences of hardness and vitreousness. Hardness is suggested to influence the adhesion forces between starch granules and protein matrix whereas vitreousness would rather be related to the endosperm microstructure.

  2. Establishment and characterization of a human uterine endometrial undifferentiated carcinoma cell line, TMG-L.

    Hasegawa, Kiyoshi; Suzuki, Machiko; Ishikawa, Kunimi; Yasue, Akira; Kato, Rina; Nakamura, Azumi; Kuroki, Jun; Udagawa, Yasuhiro

    2003-03-01

    A new cell line of human uterine endometrial undifferentiated carcinoma, designated as TMG-L, was established from the metastatic lymph node of 56-year-old patient TMG-L cells have been cultured with Ham's F-12 medium supplemented with 10% FCS and grew as a loosely adherent monolayer with polygonal or spindle-shaped cells exhibiting poor cell-cell contact and piled up against each other, showing a tendency to grow as floating cells. The doubling time of this cell line was about 48 hours, and chromosomal analysis revealed aneuploidy at passage 25. The cells formed tumors in SCID mouse, the histology of which was similar to that of undifferentiated carcinoma component of primary tumor. TMG-L cells showed the loss of expression and membranous localization of either E-cadherin or alpha-catenin, implied corresponding loss of their adhesive function. And this dysfunction implicated the biological aggressive behavior of uterine endometrial undifferentiated carcinoma. This cell line appears to provide a useful system for studying uterine undifferentiated carcinoma in vivo and in vitro.

  3. Characterization of Line Nanopatterns on Positive Photoresist Produced by Scanning Near-Field Optical Microscope

    Sadegh Mehdi Aghaei

    2015-01-01

    Full Text Available Line nanopatterns are produced on the positive photoresist by scanning near-field optical microscope (SNOM. A laser diode with a wavelength of 450 nm and a power of 250 mW as the light source and an aluminum coated nanoprobe with a 70 nm aperture at the tip apex have been employed. A neutral density filter has been used to control the exposure power of the photoresist. It is found that the changes induced by light in the photoresist can be detected by in situ shear force microscopy (ShFM, before the development of the photoresist. Scanning electron microscope (SEM images of the developed photoresist have been used to optimize the scanning speed and the power required for exposure, in order to minimize the final line width. It is shown that nanometric lines with a minimum width of 33 nm can be achieved with a scanning speed of 75 µm/s and a laser power of 113 mW. It is also revealed that the overexposure of the photoresist by continuous wave laser generated heat can be prevented by means of proper photoresist selection. In addition, the effects of multiple exposures of nanopatterns on their width and depth are investigated.

  4. Characterization of radon penetration of different structural domains of concrete. Final project report

    Nielson, K.K.; Rogers, V.C.

    1996-05-01

    This report documents the research activities by Rogers and Associates Engineering Corporation on grant DE-FG03-93ER61600 during the funded project period from August 1993 to April 1996. The objective of this research was to characterize the mechanisms and rates of radon gas penetration of the different structural domains of the concrete components of residential floor slabs, walls, and associated joints and penetrations. The research was also to characterize the physical properties of the concretes in these domains to relate their radon resistance to their physical properties. These objectives support the broader goal of characterizing which, if any, concrete domains and associated properties constitute robust barriers to radon and which permit radon entry, either inherently or in ways that could be remediated or avoided

  5. Application of frequency domain line edge roughness characterization methodology in lithography

    Sun, Lei; Wang, Wenhui; Beique, Genevieve; Wood, Obert; Kim, Ryoung-Han

    2015-03-01

    A frequency domain 3 sigma LER characterization methodology combining the standard deviation and power spectral density (PSD) methods is proposed. In the new method, the standard deviation is calculated in the frequency domain instead of the spatial domain as in the conventional method. The power spectrum of the LER is divided into three regions: low frequency (LF), middle frequency (MF) and high frequency (HF) regions. The frequency region definition is based on process visual comparisons. Three standard deviation numbers are used to characterize the LER in the three frequency regions. Pattern wiggling can be detected quantitatively with a wiggling factor which is also proposed in this paper.

  6. Establishment and characterization of a spontaneously immortalized trophoblast cell line (HPT-8) and its hepatitis B virus-expressing clone.

    Zhang, Lei; Zhang, Weilu; Shao, Chen; Zhang, Jingxia; Men, Ke; Shao, Zhongjun; Yan, Yongping; Xu, Dezhong

    2011-08-01

    Most trophoblast cell lines currently available to study vertical transmission of hepatitis B virus (HBV) are immortalized by viral transformation. Our goal was to establish and characterize a spontaneously immortalized human first-trimester trophoblast cell line and its HBV-expressing clone. Chorionic villi of Asian human first-trimester placentae were digested with trypsin and collagenase I to obtain the primary trophoblast cell culture. A spontaneously immortalized trophoblast cell line (HPT-8) was analyzed by scanning and transmission electron microscopy, cell cycle analysis, immunohistochemistry and immunofluorescence. HPT-8 cells were stably transfected with the adr subtype of HBV (HPT-8-HBV) and characterized by PCR and enzyme-linked immunosorbent assay. We obtained a clonal derivative of a spontaneously immortalized primary cell clone (HPT-8). HPT-8 cells were epithelioid and polygonal, and formed multinucleate, giant cells. They exhibited microvilli, distinct desmosomes between adjacent cells, abundant endoplasm, lipid inclusions and glycogen granules, which are all characteristic of cytotrophoblasts. HPT-8 cells expressed cytokeratin 7, cytokeratin 18, vimentin, cluster of differentiation antigen 9, epidermal growth factor receptor, stromal cell-derived factor 1 and placental alkaline phosphatase. They secreted prolactin, estradiol, progesterone and hCG, and were positive for HLA-G, a marker of extravillous trophoblasts. HPT-8-HBV cells were positive for HBV relaxed-circular, covalently closed circular DNA and pre-S sequence. HPT-8-HBV cells also produced and secreted HBV surface antigen and HBV e antigen. We established a trophoblast cell line, HPT-8 and its HBV-expressing clone which could be valuable in exploring the mechanism of HBV viral integration in human trophoblasts during intrauterine infection.

  7. Nevada Test Site Perspective on Characterization and Loading of Legacy Transuranic Drums Utilizing the Central Characterization Project

    R.G. Lahoud; J. F. Norton; I. L. Siddoway; L. W. Griswold

    2006-01-01

    The Nevada Test Site (NTS) has successfully completed a multi-year effort to characterize and ship 1860 legacy transuranic (TRU) waste drums for disposal at the Waste Isolation Pilot Plant (WIPP), a permanent TRU disposal site. This has been a cooperative effort among the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office (NNSA/NSO), the U.S. Department of Energy, Carlsbad Field Office (DOE/CBFO), the NTS Management and Operations (M and O) contractor Bechtel Nevada (BN), and various contractors under the Central Characterization Project (CCP) umbrella. The success is due primarily to the diligence, perseverance, and hard work of each of the contractors, the DOE/CBFO, and NNSA/NSO, along with the support of the U.S. Department of Energy, Headquarters (DOE/HQ). This paper presents, from an NTS perspective, the challenges and successes of utilizing the CCP for obtaining a certified characterization program, sharing responsibilities for characterization, data validation, and loading of TRU waste with BN to achieve disposal at WIPP from a Small Quantity Site (SQS) such as the NTS. The challenges in this effort arose from two general sources. First, the arrangement of DOE/CBFO contractors under the CCP performing work and certifying waste at the NTS within a Hazard Category 2 (HazCat 2) non-reactor nuclear facility operated by BN, presented difficult challenges. The nuclear safety authorization basis, safety liability and responsibility, conduct of operations, allocation and scheduling of resources, and other issues were particularly demanding. The program-level and field coordination needed for the closely interrelated characterization tasks was extensive and required considerable effort by all parties. The second source of challenge was the legacy waste itself. None of the waste was generated at the NTS. The waste was generated at Lawrence Livermore National Laboratory (LLNL), Lawrence Berkeley Laboratory (LBL), Lynchburg, Rocky

  8. Safety control system and its interface to EPICS for the off-line front end of the SPES project

    Vasquez, J.; Andrighetto, A.; Bassato, G.; Costa, L.; Giacchini, M.; Bertocco, M.

    2012-01-01

    The SPES (Selective Production of Exotic Species) project is based on a facility for the production of neutron-rich radioactive ion beams using the isotope separation on-line technique. The SPES off-line front-end apparatus involves a number of subsystems and procedures that are potentially dangerous both for human operators and for the equipment. The high voltage power supply, the ion source complex power supplies, the target chamber handling systems and the laser source are some example of these subsystems. For that reason, a safety control system has been developed. It is based on Schneider Electrics Preventa family safety modules that control the power supply of critical subsystems in combination with safety detectors that monitor critical variables. A Programmable Logic Controller (PLC), model BMXP342020 from the Schneider Electrics Modicon M340 family, is used for monitoring the status of the system as well as controlling the sequence of some operations in automatic way. A touch screen, model XBTGT5330 from the Schneider Electrics Magelis family, is used as Human Machine Interface (HMI) and communicates with the PLC using MODBUS-TCP. Additionally, an interface to the EPICS control network was developed using a home-made MODBUS-TCP EPICS driver in order to integrate it to the control system of the Front End as well as present the status of the system to the users on the main control panel. (authors)

  9. Draft site characterization analysis of the site characterization report for the Basalt Waste Isolation Project, Hanford, Washington Site. Main report and Appendices A through D

    1983-03-01

    On November 12, 1982, the US Department of Energy submitted to the US Nuclear Regulatory Commission the Site Characterization Report for the Basalt Waste Isolation Project (DOE/RL 82-3). The Basalt Waste Isolation Project is located on DOE's Hanford Reservation in the State of Washington. NUREG-0960 contains the detailed analysis, by the NRC staff, of the site characterization report. Supporting technical material is contained in Appendices A through W

  10. Characterization of the replication timing program of 6 human model cell lines

    Djihad Hadjadj

    2016-09-01

    Full Text Available During the S-phase, the DNA replication process is finely orchestrated and regulated by two programs: the spatial program that determines where replication will start in the genome (Cadoret et al. (2008 Oct 14, Cayrou et al. (2011 Sep, Picard et al. (2014 May 1 [1–3], and the temporal program that determines when during the S phase different parts of the genome are replicated and when origins are activated. The temporal program is so well conserved for each cell type from independent individuals [4] that it is possible to identify a cell type from an unknown sample just by determining its replication timing program. Moreover, replicative domains are strongly correlated with the partition of the genome into topological domains (determined by the Hi-C method, Lieberman-Aiden et al. (2009 Oct 9, Pope et al. (2014 Nov 20 [5,6]. On the one hand, replicative areas are well defined and participate in shaping the spatial organization of the genome for a given cell type. On the other hand, studies on the timing program during cell differentiation showed a certain plasticity of this program according to the stage of cell differentiation Hiratani et al. (2008 Oct 7, 2010 Feb [7,8]. Domains where a replication timing change was observed went through a nuclear re-localization. Thus the temporal program of replication can be considered as an epigenetic mark Hiratani and Gilbert (2009 Feb 16 [9]. We present the genomic data of replication timing in 6 human model cell lines: U2OS (GSM2111308, RKO (GSM2111309, HEK 293T (GSM2111310, HeLa (GSM2111311, MRC5-SV (GSM2111312 and K562 (GSM2111313. A short comparative analysis was performed that allowed us to define regions common to the 6 cell lines. These replication timing data can be taken into account when performing studies that use these model cell lines.

  11. Identification and characterization of transcript polymorphisms in soybean lines varying in oil composition and content.

    Goettel, Wolfgang; Xia, Eric; Upchurch, Robert; Wang, Ming-Li; Chen, Pengyin; An, Yong-Qiang Charles

    2014-04-23

    Variation in seed oil composition and content among soybean varieties is largely attributed to differences in transcript sequences and/or transcript accumulation of oil production related genes in seeds. Discovery and analysis of sequence and expression variations in these genes will accelerate soybean oil quality improvement. In an effort to identify these variations, we sequenced the transcriptomes of soybean seeds from nine lines varying in oil composition and/or total oil content. Our results showed that 69,338 distinct transcripts from 32,885 annotated genes were expressed in seeds. A total of 8,037 transcript expression polymorphisms and 50,485 transcript sequence polymorphisms (48,792 SNPs and 1,693 small Indels) were identified among the lines. Effects of the transcript polymorphisms on their encoded protein sequences and functions were predicted. The studies also provided independent evidence that the lack of FAD2-1A gene activity and a non-synonymous SNP in the coding sequence of FAB2C caused elevated oleic acid and stearic acid levels in soybean lines M23 and FAM94-41, respectively. As a proof-of-concept, we developed an integrated RNA-seq and bioinformatics approach to identify and functionally annotate transcript polymorphisms, and demonstrated its high effectiveness for discovery of genetic and transcript variations that result in altered oil quality traits. The collection of transcript polymorphisms coupled with their predicted functional effects will be a valuable asset for further discovery of genes, gene variants, and functional markers to improve soybean oil quality.

  12. Isolation and characterization of a neoplastic epithelial cell line derived from irradiated human submaxillary gland

    Shirasuna, Kanemitsu; Sato, Mitsunobu; Yura, Yoshiaki; Yanagawa, Tetuo; Kubo, Kazuko

    1979-01-01

    Submaxillary tissues taken from a patient whose oral base was irradiated for squamous cell carcinoma were cultured in order to isolate transformed epithelial cells in vitro. The cells showed a fine structure similar to an intermediate duct cell. When they were transplanted in nude mice, salivary tumors developed. It is epidemiologically known that irradiation induces salivary tumors. In this study, the risk of inducement was revealed and a salivary epithelial cell line was used as a model for the analysis of salivary tumors. (Ichikawa, K.)

  13. Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

    Adewumi, O.; Aflatoonian, B.; Ahrlund-Richter, L.; Amit, M.; Andrews, P.W.; Beighton, G.; Bello, P.A.; Benvenisty, N.; Berry, L.S.; Bevan, S.; Blum, B.; Brooking, J.; Chen, K.G.; Choo, A.B.H.; Churchill, G.A.; Corbel, M.; Damjanov, I.; Draper, J.S.; Dvořák, Petr; Emanuelsson, K.; Fleck, R.A.; Ford, A.; Gertow, K.; Gertsenstein, M.; Gokhale, P.J.; Hamilton, R.S.; Hampl, Aleš; Healy, L.E.; Hovatta, O.; Hyllner, J.; Imreh, M.P.; Itskovitz-Eldor, J.; Jackson, J.; Johnson, J.L.; Jones, M.; Kee, K.; King, B.L.; Knowles, B.B.; Lako, M.; Lebrin, F.; Mallon, B.S.; Manning, D.; Mayshar, Y.; Mckay, D.G.; Michalska, A.E.; Mikkola, M.; Mileikovsky, M.; Minger, S.L.; Moore, H.D.; Mummery, Ch.L.; Nagy, A.; Nakutsuji, N.; O´Brien, C.M.; Oh, S.K.W.; Olsson, C.; Otonkoski, T.; Park, K.Y.; Passier, R.; Patel, H.; Patel, M.; Pedersen, R.; Pera, M.F.; Piekarczyk, M.S.; Pera, R.A.P.; Reubinoff, B.E.; Robins, A.J.; Rossant, J.; Rugg-Gunn, P.; Schulz, T.C.; Semb, H.; Sherrer, E.S.; Siemen, H.; Stacey, G.N.; Stojkovic, M.; Suemori, H.; Szatkiewicz, J.; Turetsky, T.; Tuuri, T.; Van den Brink, S.; Vintersten, K.; Vuoristo, S.; Ward, D.; Weaver, T.A.; Young, L.A.; Zhang, W.

    2007-01-01

    Roč. 25, č. 7 (2007), s. 803-816 ISSN 1087-0156 R&D Projects: GA MŠk 1M0538; GA ČR GA301/05/0463; GA ČR GA305/05/0434 Institutional research plan: CEZ:AV0Z50390512 Keywords : International Stem Cell Initiative Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 22.848, year: 2007

  14. Characterization of HGF/Met Signaling in Cell Lines Derived From Urothelial Carcinoma of the Bladder

    Lee, Young H. [Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 (United States); Apolo, Andrea B. [Genitourinary Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 (United States); Agarwal, Piyush K.; Bottaro, Donald P., E-mail: dbottaro@helix.nih.gov [Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 (United States)

    2014-11-25

    There is mounting evidence of oncogenic hepatocyte growth factor (HGF)/Met signaling in urothelial carcinoma (UC) of the bladder. The effects of three kinase inhibitors, cabozantinib, crizotinib and EMD1214063, on HGF-driven signaling and cell growth, invasion and tumorigenicity were analyzed in cultured UC cell lines. SW780 xenograft growth in SCID and human HGF knock-in SCID (hHGF/SCID) mice treated with cabozantinib or vehicle, as well as tumor levels of Met and pMet, were also determined. Met content was robust in most UC-derived cell lines. Basal pMet content and effector activation state in quiescent cells were low, but significantly enhanced by added HGF, as were cell invasion, proliferation and anchorage independent growth. These HGF-driven effects were reversed by Met inhibitor treatment. Tumor xenograft growth was significantly higher in hHGF/SCID mice vs. SCID mice and significantly inhibited by cabozantinib, as was tumor phospho-Met content. These studies indicate the prevalence and functionality of the HGF/Met signaling pathway in UC cells, suggest that paracrine HGF may contribute to UC tumor growth and progression, and that support further preclinical investigation of Met inhibitors for the treatment of UC is warranted.

  15. Establishment and mitotic characterization of new Drosophila acentriolar cell lines from DSas-4 mutant

    Nicolas Lecland

    2013-01-01

    In animal cells the centrosome is commonly viewed as the main cellular structure driving microtubule (MT assembly into the mitotic spindle apparatus. However, additional pathways, such as those mediated by chromatin and augmin, are involved in the establishment of functional spindles. The molecular mechanisms involved in these pathways remain poorly understood, mostly due to limitations inherent to current experimental systems available. To overcome these limitations we have developed six new Drosophila cell lines derived from Drosophila homozygous mutants for DSas-4, a protein essential for centriole biogenesis. These cells lack detectable centrosomal structures, astral MT, with dispersed pericentriolar proteins D-PLP, Centrosomin and γ-tubulin. They show poorly focused spindle poles that reach the plasma membrane. Despite being compromised for functional centrosome, these cells could successfully undergo mitosis. Live-cell imaging analysis of acentriolar spindle assembly revealed that nascent MTs are nucleated from multiple points in the vicinity of chromosomes. These nascent MTs then grow away from kinetochores allowing the expansion of fibers that will be part of the future acentriolar spindle. MT repolymerization assays illustrate that acentriolar spindle assembly occurs “inside-out” from the chromosomes. Colchicine-mediated depolymerization of MTs further revealed the presence of a functional Spindle Assembly Checkpoint (SAC in the acentriolar cells. Finally, pilot RNAi experiments open the potential use of these cell lines for the molecular dissection of anastral pathways in spindle and centrosome assembly.

  16. Characterization of the microDNA through the response to chemotherapeutics in lymphoblastoid cell lines.

    Pamela Mehanna

    Full Text Available Recently, a new class of extrachromosomal circular DNA, called microDNA, was identified. They are on average 100 to 400 bp long and are derived from unique non-repetitive genomic regions with high gene density. MicroDNAs are thought to arise from DNA breaks associated with RNA metabolism or replication slippage. Given the paucity of information on this entirely novel phenomenon, we aimed to get an additional insight into microDNA features by performing the microDNA analysis in 20 independent human lymphoblastoid cell lines (LCLs prior and after treatment with chemotherapeutic drugs. The results showed non-random genesis of microDNA clusters from the active regions of the genome. The size periodicity of 190 bp was observed, which matches DNA fragmentation typical for apoptotic cells. The chemotherapeutic drug-induced apoptosis of LCLs increased both number and size of clusters further suggesting that part of microDNAs could result from the programmed cell death. Interestingly, proportion of identified microDNA sequences has common loci of origin when compared between cell line experiments. While compatible with the original observation that microDNAs originate from a normal physiological process, obtained results imply complementary source of its production. Furthermore, non-random genesis of microDNAs depicted by redundancy between samples makes these entities possible candidates for new biomarker generation.

  17. Site characterization quality assurance for the California LLRW Disposal Site Project

    Hanrahan, T.P.; Ench, J.E.; Serlin, C.L.; Bennett, C.B.

    1988-01-01

    In December of 1985 US Ecology was chosen as the license designee for the State of California's low-level radioactive waste disposal facility. In early 1987, three candidate sites were selected for characterization studies in preparation for identifying the preferred site. The geotechnical characterization activities along with studies of the ecological and archaeological attributes, as well as assessments of the socio-economic impacts and cultural resources all provide input towards selection of the proposed site. These technical studies in conjunction with comments from local citizen committees and other interested parties are used as a basis for determining the proposed site for which full site characterization as required by California licensing requirements are undertaken. The purpose of this paper is to present an overview of the program for Quality Assurance and Quality Control for the site characterization activities on the California LLRW Disposal Site Project. The focus is on three major perspectives: The composite QA Program and two of the primary characterization activities, the geotechnical and the meteorological investigations

  18. Engineered materials characterization report for the Yucca Mountain Site Characterization Project. Volume 1, Introduction, history, and current candidates

    Van Konynenburg, R.A.; McCright, R.D.; Roy, A.K.; Jones, D.A.

    1995-08-01

    The purpose of the Yucca Mountain Site Characterization Project is to evaluate Yucca Mountain for its suitability as a potential site for the nation's first high-level nuclear waste repository. As part of this effort, Lawrence Livermore National Laboratory (LLNL) has been occupied for a number of years with developing and evaluating the performance of waste packages for the potential repository. In recent years this work has been carried out under the guidance of and in collaboration with the Management and Operating contractor for the Civilian Radioactive Waste Management System, TRW Environmental Safety Systems, Inc., which in turn reports to the Office of Civilian Radioactive Waste Management of the US Department of Energy. This report summarizes the history of the selection and characterization of materials to be used in the engineered barrier system for the potential repository at Yucca Mountain, describes the current candidate materials, presents a compilation of their properties, and summarizes available corrosion data and modeling. The term ''engineered materials'' is intended to distinguish those materials that are used as part of the engineered barrier system from the natural, geologic materials of the site

  19. Genome-wide characterization of genetic variants and putative regions under selection in meat and egg-type chicken lines.

    Boschiero, Clarissa; Moreira, Gabriel Costa Monteiro; Gheyas, Almas Ara; Godoy, Thaís Fernanda; Gasparin, Gustavo; Mariani, Pilar Drummond Sampaio Corrêa; Paduan, Marcela; Cesar, Aline Silva Mello; Ledur, Mônica Corrêa; Coutinho, Luiz Lehmann

    2018-01-25

    Meat and egg-type chickens have been selected for several generations for different traits. Artificial and natural selection for different phenotypes can change frequency of genetic variants, leaving particular genomic footprints throghtout the genome. Thus, the aims of this study were to sequence 28 chickens from two Brazilian lines (meat and white egg-type) and use this information to characterize genome-wide genetic variations, identify putative regions under selection using Fst method, and find putative pathways under selection. A total of 13.93 million SNPs and 1.36 million INDELs were identified, with more variants detected from the broiler (meat-type) line. Although most were located in non-coding regions, we identified 7255 intolerant non-synonymous SNPs, 512 stopgain/loss SNPs, 1381 frameshift and 1094 non-frameshift INDELs that may alter protein functions. Genes harboring intolerant non-synonymous SNPs affected metabolic pathways related mainly to reproduction and endocrine systems in the white-egg layer line, and lipid metabolism and metabolic diseases in the broiler line. Fst analysis in sliding windows, using SNPs and INDELs separately, identified over 300 putative regions of selection overlapping with more than 250 genes. For the first time in chicken, INDEL variants were considered for selection signature analysis, showing high level of correlation in results between SNP and INDEL data. The putative regions of selection signatures revealed interesting candidate genes and pathways related to important phenotypic traits in chicken, such as lipid metabolism, growth, reproduction, and cardiac development. In this study, Fst method was applied to identify high confidence putative regions under selection, providing novel insights into selection footprints that can help elucidate the functional mechanisms underlying different phenotypic traits relevant to meat and egg-type chicken lines. In addition, we generated a large catalog of line-specific and common

  20. Characterizing changes in the excitability of corticospinal projections to proximal muscles of the upper limb.

    Carson, Richard G; Nelson, Barry D; Buick, Alison R; Carroll, Timothy J; Kennedy, Niamh C; Cann, Rachel Mac

    2013-09-01

    There has been an explosion of interest in methods of exogenous brain stimulation that induce changes in the excitability of human cerebral cortex. The expectation is that these methods may promote recovery of function following brain injury. To assess their effects on motor output, it is typical to assess the state of corticospinal projections from primary motor cortex to muscles of the hand, via electromyographic responses to transcranial magnetic stimulation. If a range of stimulation intensities is employed, the recruitment curves (RCs) obtained can, at least for intrinsic hand muscles, be fitted by a sigmoid function. To establish whether sigmoid fits provide a reliable basis upon which to characterize the input-output properties of the corticospinal pathway for muscles proximal to the hand, and to assess as an alternative the area under the (recruitment) curve (AURC). A comparison of the reliability of these measures, using RCs obtained for muscles that are frequently the targets of rehabilitation. The AURC is an extremely reliable measure of the state of corticospinal projections to hand and forearm muscles, which has both face and concurrent validity. Construct validity is demonstrated by detection of widely distributed (across muscles) changes in corticospinal excitability induced by paired associative stimulation (PAS). The parameters derived from sigmoid fits are unlikely to provide an adequate means to assess the effectiveness of therapeutic regimes. The AURC can be employed to characterize corticospinal projections to a range of muscles, and gauge the efficacy of longitudinal interventions in clinical rehabilitation. Copyright © 2013 Elsevier Inc. All rights reserved.

  1. Radiostatine and radioiodine uptake characterization in sodium iodine symporter-expressing cell lines

    Petrich, T.; Helmeke, H.J.; Meyer, G.J.; Knapp, W.H.; Poetter, E.

    2002-01-01

    Full text: The sodium iodide symporter (NIS) has been recognized as an attractive target for cancer gene therapy. Here we investigated NIS-mediated transport of the high LET α-emitter astatine, 211 At, in comparison to radioiodine. A constitutive expression vector harbouring the human NIS cDNA was used in combination with reporter gene vectors for transient transfection of 13 different human cancer cell lines. Radioiodine uptake was measured as well as transfection efficiencies. Six stable NIS-expressing cell lines (3 derived from thyroid carcinomas, 2 colon carcinoma, 1 glioblastoma) were generated by antibiotic selection. NIS expression was monitored by immunohistochemistry and RT-PCR. Subsequently the radioastatine and radioiodine uptake characteristics of genetically modified cells were studied in comparison to the respective control cells. After xenotransplantation in nude mice in vivo tumor imaging by scintigraphy and biodistribution studies following organ removal were performed. Transient transfection of NIS cDNA led to high specific sodium perchlorate-sensitive radioiodine uptake in NIS-expressing cells that roughly correlates to transfection efficiencies. Similarly, stable NIS-expressing cell lines were able to concentrate high levels of radioiodine and in addition showed comparable transport capacity for radioastatine. Accumulation of 211 At was inhibited by sodium perchlorate like iodide uptake and displayed dependency an extracellular Na + - and I - -ions as well. Compared to wash-out experiments in cell culture the effective half life of radioiodine and radioastatine in vivo was significantly prolonged. Preliminary dose calculations by MIRD concepts indicated higher tumor radiation doses for 211 At compared to 131 I. Tumor cells of different origins transfected with the NIS-expression vector specifically and significantly take-up radioiodine and radioastatine in vitro and in vivo. The data provide direct evidence that the NIS efficiently transports

  2. Characterization of the Process Mechanical Cell at the West Valley Demonstration Project

    Drake, John; Schneider, Ken; Choroser, Jeff; Hughes, Karl

    2003-01-01

    The West Valley Demonstration Project has initiated decontamination and dismantlement (D and D) of the most highly radioactive and contaminated cells in a former spent nuclear fuel reprocessing plant. The goals of the D and D project are to remove loose debris in the cells and estimate the residual radioactivity level of legacy plant equipment. To support accomplishment of these goals, a unique characterization approach was developed to gather the information to meet anticipated Waste Isolation Pilot Plant (WIPP) acceptance criteria for remote-handled transuranic waste, and to facilitate segregation and packaging operations. Implementation of the characterization approach included the development and use of innovative, remote technology for measuring gamma radiation within the hot cell. The technology was used to identify and quantify radiation from individual debris items in radiation fields up to 2,000 R/hr (20 sieverts/hr). Sampling and analysis of the debris were also performed via remote handling means. Significant challenges associated with characterizing the highly radioactive and highly contaminated hot cells were encountered. The innovative solutions for meeting these challenges are applicable throughout the Department of Energy Complex and help support the goal of targeting D and D efforts toward reducing risks to public health and the environment

  3. Generation and Characterization of Vascular Smooth Muscle Cell Lines Derived from a Patient with a Bicuspid Aortic Valve

    Pamela Lazar-Karsten

    2016-04-01

    Full Text Available Thoracic aortic dilation is the most common malformation of the proximal aorta and is responsible for 1%–2% of all deaths in industrialized countries. In approximately 50% of patients with a bicuspid aortic valve (BAV, dilation of any or all segments of the aorta occurs. BAV patients with aortic dilation show an increased incidence of cultured vascular smooth muscle cell (VSMC loss. In this study, VSMC, isolated from the ascending aorta of BAV, was treated with Simian virus 40 to generate a BAV-originated VSMC cell line. To exclude any genomic DNA or cross-contamination, highly polymorphic short tandem repeats of the cells were profiled. The cells were then characterized using flow cytometry and karyotyping. The WG-59 cell line created is the first reported VSMC cell line isolated from a BAV patient. Using an RT2 Profiler PCR Array, genes within the TGFβ/BMP family that are dependent on losartan treatment were identified. Endoglin was found to be among the regulated genes and was downregulated in WG-59 cells following treatment with different losartan concentrations, when compared to untreated WG-59 cells.

  4. Proposal for characterization of muon spectrometers for neutrino beam lines with the Baby MIND

    Noah, E

    2015-01-01

    Neutrino detectors based on state-of-the-art plastic scintillators read out with solid state photo-sensors, as well as new magnetization schemes, have been developed in the framework of AIDA. Meaningful size prototypes are under construction. In the framework of the CERN neutrino platform, we propose to test a Totally Active Scintillator Detector (TASD) and a prototype of a Magnetized Iron Neutrino Detector (MIND), called Baby MIND in the H8 beam line in 2016-2018. The design of the detectors and the purpose and plans for the beam tests are presented. An opportunity to use the Baby MIND detector in a real neutrino beam at JPARC for the measurement of the cross-section ratio between Water and scintillator (WAGASCI experiment) is described.

  5. Characterization of electron beams generated in a high-voltage pulse-line-driven pseudospark discharge

    Ramaswamy, K.; Destler, W.W.; Segalov, Z.; Rodgers, J.

    1994-01-01

    Emittance and energy measurements have been performed on a high-brightness electron beam (>10 10 A/m 2 rad 2 ) with diameter in the range 1--3 mm and energy in the range 150--170 keV. This electron beam is generated by the mating of a hollow-cathode discharge device operating in the pseudospark regime to the output of a high-power pulse line accelerator. The measured effective emittance lies in the range between 30 and 90 mm mrad and increases with axial distance. Electron energy measurements indicate that the high-energy electrons are generated during the first 20--30 ns of the discharge. Both the emittance and energy experiments were performed at two different ambient argon gas pressures (92 and 152 mtorr). Beam expansion as a function of axial position has also been studied and a lower bound on the beam brightness has been obtained

  6. The in silico identification and characterization of a bread wheat/Triticum militinae introgression line

    Abrouk, Michael; Balcárková, Barbora; Šimková, Hana; Komínková, Eva; Martis, M.M.; Jakobson, I.; Timofejeva, L.; Rey, Elodie; Vrána, Jan; Kilian, A.; Jarve, K.; Doležel, Jaroslav; Valárik, Miroslav

    2017-01-01

    Roč. 15, č. 2 (2017), s. 249-256 ISSN 1467-7644 R&D Projects: GA MŠk(CZ) LO1204; GA ČR(CZ) GA14-07164S Institutional support: RVO:61389030 Keywords : crop improvement * powdery mildew * common wheat * chromosomes * genome * resistance * plant * recombination * evolution * barley * GenomeZipper * alien introgression * comparative analysis * chromosome rearrangement * chromosome translocation * linkage drag Subject RIV: EB - Gene tics ; Molecular Biology OBOR OECD: Plant sciences, botany Impact factor: 7.443, year: 2016

  7. Characterization of three human cell line models for high-throughput neuronal cytotoxicity screening.

    Tong, Zhi-Bin; Hogberg, Helena; Kuo, David; Sakamuru, Srilatha; Xia, Menghang; Smirnova, Lena; Hartung, Thomas; Gerhold, David

    2017-02-01

    More than 75 000 man-made chemicals contaminate the environment; many of these have not been tested for toxicities. These chemicals demand quantitative high-throughput screening assays to assess them for causative roles in neurotoxicities, including Parkinson's disease and other neurodegenerative disorders. To facilitate high throughput screening for cytotoxicity to neurons, three human neuronal cellular models were compared: SH-SY5Y neuroblastoma cells, LUHMES conditionally-immortalized dopaminergic neurons, and Neural Stem Cells (NSC) derived from human fetal brain. These three cell lines were evaluated for rapidity and degree of differentiation, and sensitivity to 32 known or candidate neurotoxicants. First, expression of neural differentiation genes was assayed during a 7-day differentiation period. Of the three cell lines, LUHMES showed the highest gene expression of neuronal markers after differentiation. Both in the undifferentiated state and after 7 days of neuronal differentiation, LUHMES cells exhibited greater cytotoxic sensitivity to most of 32 suspected or known neurotoxicants than SH-SY5Y or NSCs. LUHMES cells were also unique in being more susceptible to several compounds in the differentiating state than in the undifferentiated state; including known neurotoxicants colchicine, methyl-mercury (II), and vincristine. Gene expression results suggest that differentiating LUHMES cells may be susceptible to apoptosis because they express low levels of anti-apoptotic genes BCL2 and BIRC5/survivin, whereas SH-SY5Y cells may be resistant to apoptosis because they express high levels of BCL2, BIRC5/survivin, and BIRC3 genes. Thus, LUHMES cells exhibited favorable characteristics for neuro-cytotoxicity screening: rapid differentiation into neurons that exhibit high level expression neuronal marker genes, and marked sensitivity of LUHMES cells to known neurotoxicants. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  8. Extending i-line capabilities through variance characterization and tool enhancement

    Miller, Dan; Salinas, Adrian; Peterson, Joel; Vickers, David; Williams, Dan

    2006-03-01

    Continuous economic pressures have moved a large percent of integrated device manufacturing (IDM) operations either overseas or to foundry operations over the last 10 years. These pressures have left the IDM fabs in the U.S. with required COO improvements in order to maintain operations domestically. While the assets of many of these factories are at a very favorable point in the depreciation life cycle, the equipment and processes are constrained to the quality of the equipment in its original state and the degradation over its installed life. With the objective to enhance output and improve process performance, this factory and their primary lithography process tool supplier have been able to extend the usable life of the existing process tools, increase the output of the tool base, and improve the distribution of the CDs on the product produced. Texas Instruments Incorporated lead an investigation with the POLARIS ® Systems & Services business of FSI International to determine the sources of variance in the i-line processing of a wide array of IC device types. Data from the sources of variance were investigated such as PEB temp, PEB delay time, develop recipe, develop time, and develop programming. While PEB processes are a primary driver of acid catalyzed resists, the develop mode is shown in this work to have an overwhelming impact on the wafer to wafer and across wafer CD performance of these i-line processes. These changes have been able to improve the wafer to wafer CD distribution by more than 80 %, and the within wafer CD distribution by more than 50 % while enabling a greater than 50 % increase in lithography cluster throughput. The paper will discuss the contribution from each of the sources of variance and their importance in overall system performance.

  9. Characterization of the RAPD for 6 Durum wheat lines (Triticum durum desf.) selected from M4-irradiated population under drought conditions

    Kalil, M.K.; Nesiem, M.R.A.; Kassem, M.K.M.; Basyouny, M.A.E.

    2012-01-01

    Grains of two durum wheat cultivars Sohag 3 and Beni Suef 3 were irradiated with different doses of gamma ray 0, 150, 250 and 350 Gy to obtain new durum wheat lines characterized by high yielding and drought tolerance. Irradiated grins were cultivated in the field under normal and drought conditions during 2005-2009 Results of field experiments showed that there were new six putative lines S1, S2, S3, S4, B1 and B2. Each putative line had superiority than its parent in grain yield / plant. The putative lines S1 and S2 had superiority over their parent Sohag 3 under normal conditions in grain yield per plant this increase equal 52 and 60% respectively. The putative lines S3 and S4 had superiority in grain yield per plan as compared to Sohag 3 under drought conditions this increase equal 75 to 58% respectively. The putative lines B1 and B2 had superiority in grain yield per plant than their parent Beni Suef 3 under normal condition this increase equal 46 and 12% respectively. Results for RAPD markers showed that each putative line was characterized by positive and negative unique marker. The putative line S1: characterized by four negative marker amplified by OPM-05, OPN-04, OPA-18 and OPB-12 primers. The putative line S2: characterized by one negative unique marker amplified by OPQ-14 marker. The putative line S3: characterized by two positive markers amplified by OPB-07 and OPG-12 markers and one negative unique marker amplified by OPA-10 marker. The putative line S4: characterized by four negative markers amplified by OPM-05, OPN-13, OPQ-12 and OPQ-14 markers and one positive unique marker amplified by OPC-05 marker. The putative line B1: characterized by four positive markers amplified by OPA-10, OPG-12, OPB-07 and OPA-18 markers and three negative markers amplified with OPM-05, OPC-05 and OPB-12 markers. The putative line B2: characterized by three positive unique marker amplified by OPB-07, OPN-04, OPN-10 markers and four negative markers amplified with OPA-10

  10. Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung

    Kalinina, Tatyana; Simon, Ronald; Otto, Benjamin; Dierlamm, Judith; Schwarzenbach, Heidi; Effenberger, Katharina E; Bockhorn, Maximilian; Izbicki, Jakob R; Yekebas, Emre F; Güngör, Cenap; Thieltges, Sabrina; Möller-Krull, Maren; Murga Penas, Eva Maria; Wicklein, Daniel; Streichert, Thomas; Schumacher, Udo; Kalinin, Viacheslav

    2010-01-01

    Pancreatic cancer is still associated with devastating prognosis. Real progress in treatment options has still not been achieved. Therefore new models are urgently needed to investigate this deadly disease. As a part of this process we have established and characterized a new human pancreatic cancer cell line. The newly established pancreatic cancer cell line PaCa 5061 was characterized for its morphology, growth rate, chromosomal analysis and mutational analysis of the K-ras, EGFR and p53 genes. Gene-amplification and RNA expression profiles were obtained using an Affymetrix microarray, and overexpression was validated by IHC analysis. Tumorigenicity and spontaneous metastasis formation of PaCa 5061 cells were analyzed in pfp -/- /rag2 -/- mice. Sensitivity towards chemotherapy was analysed by MTT assay. PaCa 5061 cells grew as an adhering monolayer with a doubling time ranging from 30 to 48 hours. M-FISH analyses showed a hypertriploid complex karyotype with multiple numerical and unbalanced structural aberrations. Numerous genes were overexpressed, some of which have previously been implicated in pancreatic adenocarcinoma (GATA6, IGFBP3, IGFBP6), while others were detected for the first time (MEMO1, RIOK3). Specifically highly overexpressed genes (fold change > 10) were identified as EGFR, MUC4, CEACAM1, CEACAM5 and CEACAM6. Subcutaneous transplantation of PaCa 5061 into pfp -/- /rag2 -/- mice resulted in formation of primary tumors and spontaneous lung metastasis. The established PaCa 5061 cell line and its injection into pfp -/- /rag2 -/- mice can be used as a new model for studying various aspects of the biology of human pancreatic cancer and potential treatment approaches for the disease

  11. Establishment and characterization of novel epithelial-like cell lines derived from human periodontal ligament tissue in vitro.

    Tansriratanawong, Kallapat; Ishikawa, Hiroshi; Toyomura, Junko; Sato, Soh

    2017-10-01

    In this study, novel human-derived epithelial-like cells (hEPLCs) lines were established from periodontal ligament (PDL) tissues, which were composed of a variety of cell types and exhibited complex cellular activities. To elucidate the putative features distinguishing these from epithelial rest of Malassez (ERM), we characterized hEPLCs based on cell lineage markers and tight junction protein expression. The aim of this study was, therefore, to establish and characterize hEPLCs lines from PDL tissues. The hEPLCs were isolated from PDL of third molar teeth. Cellular morphology and cell organelles were observed thoroughly. The characteristics of epithelial-endothelial-mesenchymal-like cells were compared in several markers by gene expression and immunofluorescence, to ERM and human umbilical-vein endothelial cells (HUVECs). The resistance between cellular junctions was assessed by transepithelial electron resistance, and inflammatory cytokines were detected by ELISA after infecting hEPLCs with periodontopathic bacteria. The hEPLCs developed into small epithelial-like cells in pavement appearance similar to ERM. However, gene expression patterns and immunofluorescence results were different from ERM and HUVECs, especially in tight junction markers (Claudin, ZO-1, and Occludins), and endothelial markers (vWF, CD34). The transepithelial electron resistance indicated higher resistance in hEPLCs, as compared to ERM. Periodontopathic bacteria were phagocytosed with upregulation of inflammatory cytokine secretion within 24 h. In conclusion, hEPLCs that were derived using the single cell isolation method formed tight multilayers colonies, as well as strongly expressed tight junction markers in gene expression and immunofluorescence. Novel hEPLCs lines exhibited differently from ERM, which might provide some specific functions such as metabolic exchange and defense mechanism against bacterial invasion in periodontal tissue.

  12. High Line

    Kiib, Hans

    2015-01-01

    At just over 10 meters above street level, the High Line extends three kilometers through three districts of Southwestern Manhattan in New York. It consists of simple steel construction, and previously served as an elevated rail line connection between Penn Station on 34th Street and the many....... The High Line project has been carried out as part of an open conversion strategy. The result is a remarkable urban architectural project, which works as a catalyst for the urban development of Western Manhattan. The greater project includes the restoration and reuse of many old industrial buildings...

  13. Characterization of the Mucor circinelloides life cycle by on-line image analysis

    Lübbehüsen, Tina Louise; Nielsen, Jens; Mcintyre, Mhairi

    2003-01-01

    in and between the different morphological forms of the organism.Methods and Results: Mycelial growth and the transformation of hyphae into chains of arthrospores were characterized by image analysis techniques and described quantitatively. The influence of the nature (glucose and xylose) and concentration......-through cell, and combined with fluorescent microscopy which allowed new insights to bud formation. Additionally, numbers and distribution of nuclei in arthrospores, hyphae and yeasts were studied.Conclusions: The results give essential information on the morphological development of the organism...

  14. NEOShield-2 Project: Final Results on Compositional Characterization of small NEOs

    Barucci, Maria Antonieta; Perna, Davide; Fornasier, Sonia; Doressoundiram, Alain; Lantz, Cateline; Popescu, Marcel; Merlin, Frederic; Fulchignoni, Marcello

    2017-10-01

    NEOShield-2 project was selected in the framework of the European Commission H2020 program in answer to the call for “Access technologies and characterisation for Near Earth Objects (NEOs)”. NEOShield-2 project (2015-2017) is a follow-up of the first NEOShield (2012-2015) and includes 11 European Institutions and Industries. The main objectives of NEOShield-2 project are: i) technological development on techniques and instruments needed for GNC for possible asteroid missions and ii) characterization of NEOs of small sizes.Our team at LESIA is the leader of the entire observational program which involved complementary techniques to provide physical and compositional characterization of NEOs. Priority has been given to potential space-mission targets, optimized for mitigation or exploration missions. In this framework an agreement with the European Southern Observatory was signed to obtain Guaranteed Time Observations at the 3.6-meter NTT with an allocation of 30 nights to characterize by spectroscopy the composition of the smaller asteroids. The objects with an absolute magnitude larger than 20 were selected, with a priority for the very small newly discovered objects.We obtained more than 170 new spectra of NEOs. The observations were performed with EFOSC2 instrument. We covered the wavelength interval 0.4-0.92 microns, with a resolution of R=~200. The observed asteroids include 29 asteroids with diameters smaller than 100 meters and 71 with diameters between 100 and 300 m.The taxonomic type has been assigned for 137 individual objects. Our results on NEO mineralogical compositions provide a body of reference data directly applicable to the design and development of mitigation-relevant space missions. Within our survey, we found eight D-types with ΔV funding by European Commission Horizon 2020 program (contract No. PROTEC-2-2014-640351).

  15. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1993 Quality Program status report

    Boliver, S.L.

    1995-05-01

    This status report is for calendar year 1993. It summarizes the annual activities and accomplishments of the Los Alamos National Laboratory (Los Alamos) Yucca Mountain Site Characterization Project (YMP or Project) quality assurance program. By identifying the accomplishments of the quality program, we establish a baseline that will assist in decision making, improve administrative controls and predictability, and allow us to annually identify long term trends and to evaluate improvements. This is the third annual status report (Bolivar, 1992; Bolivar, 1994). This report is divided into two primary sections: Program Activities and Trend Analysis. Under Program Activities, programmatic issues occurring in 1993 are discussed. The goals for 1993 are also listed, followed by a discussion of their status. Lastly, goals for 1994 are identified. The Trend Analysis section is a summary of 1993 quarterly trend reports and provides a good overview of the quality assurance issues of the Los Alamos YMP

  16. The MEarth Project: Finding the Best Targets for Atmospheric Characterization with JWST

    Berta-Thompson, Z.

    2014-04-01

    If we want to directly observe the radius, orbit, mass, and atmosphere of a small, cool, habitable exoplanet, our best opportunity is to find such a planet transiting a small, cool, nearby M dwarf star. The MEarth Project is an ongoing all-sky survey for Earth-like planets transiting the closest, smallest M dwarfs in the Galaxy. MEarth aims to find good targets for atmospheric characterization with JWST and the next generation of enormous ground-based telescopes. This poster provides a status update on the MEarth Project, including the progress we've made over the past five years with 8 telescopes in the Northern hemisphere and promising early results from our new installation of 8 more telescopes in the Southern hemisphere.

  17. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1993 Quality Program status report

    Bolivar, S.L.

    1995-05-01

    This status report is for calendar year 1993. It summarizes the annual activities and accomplishments of the Los Alamos National Laboratory (Los Alamos) Yucca Mountain Site Characterization Project (YMP or Project) quality assurance program. By identifying the accomplishments of the quality program, we establish a baseline that will assist in decision making, improve administrative controls and predictability, and allow us to annually identify long term trends and to evaluate improvements. This is the third annual status report (Bolivar, 1992; Bolivar, 1994). This report is divided into two primary sections: Program Activities and Trend Analysis. Under Program Activities, programmatic issues occurring in 1993 are discussed. The goals for 1993 are also listed, followed by a discussion of their status. Lastly, goals for 1994 are identified. The Trend Analysis section is a summary of 1993 quarterly trend reports and provides a good overview of the quality assurance issues of the Los Alamos YMP.

  18. Fabrication, characterization and testing of silicon photomultipliers for the Muon Portal Project

    La Rocca, P.; Billotta, S.; Blancato, A.A.; Bonanno, D.; Bonanno, G.; Fallica, G.; Garozzo, S.; Lo Presti, D.; Marano, D.; Pugliatti, C.; Riggi, F.; Romeo, G.; Santagati, G.; Valvo, G.

    2015-01-01

    The Muon Portal is a recently started Project aiming at the construction of a large area tracking detector that exploits the muon tomography technique to inspect the contents of traveling cargo containers. The detection planes will be made of plastic scintillator strips with embedded wavelength-shifting fibres. Special designed silicon photomultipliers will read the scintillation light transported by the fibres along the strips and a dedicated electronics will combine signals from different strips to reduce the overall number of channels, without loss of information. Different silicon photomultiplier prototypes, both with the p-on-n and n-on-p technologies, have been produced by STMicroelectronics during the last years. In this paper we present the main characteristics of the silicon photomultipliers designed for the Muon Portal Project and describe the setup and the procedure implemented for the characterization of these devices, giving some statistical results obtained from the test of a first batch of silicon photomultipliers

  19. Fabrication, characterization and testing of silicon photomultipliers for the Muon Portal Project

    La Rocca, P., E-mail: paola.larocca@ct.infn.it [Dipartimento di Fisica e Astronomia - Catania (Italy); INFN - Sezione di Catania (Italy); Billotta, S. [INAF - Osservatorio Astrofisico di Catania (Italy); Blancato, A.A.; Bonanno, D. [Dipartimento di Fisica e Astronomia - Catania (Italy); Bonanno, G. [INAF - Osservatorio Astrofisico di Catania (Italy); Fallica, G. [STMicroelectronics - Catania (Italy); Garozzo, S. [INAF - Osservatorio Astrofisico di Catania (Italy); Lo Presti, D. [Dipartimento di Fisica e Astronomia - Catania (Italy); INFN - Sezione di Catania (Italy); Marano, D. [INAF - Osservatorio Astrofisico di Catania (Italy); Pugliatti, C.; Riggi, F. [Dipartimento di Fisica e Astronomia - Catania (Italy); INFN - Sezione di Catania (Italy); Romeo, G. [INAF - Osservatorio Astrofisico di Catania (Italy); Santagati, G. [Dipartimento di Fisica e Astronomia - Catania (Italy); INFN - Sezione di Catania (Italy); Valvo, G. [STMicroelectronics - Catania (Italy)

    2015-07-01

    The Muon Portal is a recently started Project aiming at the construction of a large area tracking detector that exploits the muon tomography technique to inspect the contents of traveling cargo containers. The detection planes will be made of plastic scintillator strips with embedded wavelength-shifting fibres. Special designed silicon photomultipliers will read the scintillation light transported by the fibres along the strips and a dedicated electronics will combine signals from different strips to reduce the overall number of channels, without loss of information. Different silicon photomultiplier prototypes, both with the p-on-n and n-on-p technologies, have been produced by STMicroelectronics during the last years. In this paper we present the main characteristics of the silicon photomultipliers designed for the Muon Portal Project and describe the setup and the procedure implemented for the characterization of these devices, giving some statistical results obtained from the test of a first batch of silicon photomultipliers.

  20. Technical data base quarterly report, April--June 1992; Yucca Mountain Site Characterization Project

    NONE

    1992-09-01

    The acquisition and development of technical data are activities that provide the information base from which the Yucca mountain Site will be characterized and may P-ventually be licensed as a high-level waste repository. The Project Technical Data Base (TDB) is the repository for the regional and site-specific technical data required in intermediate and license application analyses and models. The TDB Quarterly Report provides the mechanism for identifying technical data currently available from the Project TDB. Due to the variety of scientific information generated by YMP activities, the Project TDB consists of three components, each designed to store specific types of data. The Site and Engineering Properties Data Base (SEPDB) maintains technical data best stored in a tabular format. The Geographic Nodal Information Study and Evaluation System (GENISES), which is the Geographic Information System (GIS) component of the Project TDB, maintains spatial or map-like data. The Geologic and Engineering Materials Bibliography of Chemical Species (GEMBOCHS) data base maintains thermodynamic/geochemical data needed to support geochemical reaction models involving the waste package and repository geochemical environment. Each of these data bases are addressed independently within the TDB Quarterly Report.

  1. Control of tracers, fluids, and materials for the Yucca Mountain Site Characterization Project

    Kalia, H.N.

    1993-01-01

    This paper describes use and control of tracers, fluids, and materials (TFM) at the Yucca Mountain Site Characterization Project, Management of TFM is necessary to ensure that site characterization activity does not introduce TFM that may have impact on Yucca Mountain's ability to isolate high-level radioactive waste from the accessible environment. All participants must identify TFM used for testing and construction and have the TFM evaluated to ascertain any impact on waste isolation capabilities of the site or on adjacent tests. Two data bases are created to track TFM: a working data base managed by Los Alamos National Lab. and a permanent data base managed by EG ampersand G, which will contain information on actual TFM used

  2. Response to state comments on the draft regional characterization reports for the Crystalline Repository Project

    1984-11-01

    In May, 1983, the US Department of Energy (DOE) Crystalline Repository Project Office (CPO) issued six draft Regional Characterization Reports (RCRs) for review and comment to the 17 Crystalline States comprising the Northeastern, Southeastern, and North Central crystalline regions. Comment letters were received from 13 of the 17 states. The more than 2000 comments generally focused on the quality and content of the characterization reports and on their intended use in region-to-area screening. These comments were paraphrased and grouped into 23 subjects within the following four topical areas: (1) General and Programmatic; (2) Geologic; (3) Environmental and Socioeconomic; and (4) Editorial. This document provides responses to the comments submitted by the states

  3. Probabilistic comparison of alternative characterization technologies at the Fernald Uranium-in-Soils Integrated Demonstration Project

    Rautman, C.A.; McGraw, M.A.; Istok, J.D.; Sigda, J.M.; Kaplan, P.G.

    1993-01-01

    The performance of four alternative characterization technologies proposed for use in characterization of surficial uranium contamination in soil at the Incinerator and Drum Baling Areas at the Fernald Environmental Management Project in southwestern Ohio has been evaluated using a probabilistic, risk-based decision-analysis methodology. The basis of comparison is to minimize a computed total cost for environmental cleanup. This total-cost-based approach provides a framework for evaluating the trade-offs among remedial investigation, the remedial design, and the risk of regulatory penalties. The approach explicitly recognizes the value of information provided by remedial investigation; additional measurements are only valuable to the extent that the information they provide reduces total cost

  4. Test and characterization of multigap resistive plate chambers for the EEE project

    Bossini, E

    2016-01-01

    The Extreme Energy Events project is based on the deployment of cosmic-ray telescopes in Italian high schools with the active contribution of students and teachers. Each telescope is made by three Multigap Resistive Plate Chambers readout by strips. With around 50 telescopes already built and others under construction, specific systems to test and characterize the chambers are needed. In this article I will present a flexible and software-configurable solution to perform chamber efficiency studies with a set of scintillators and hardware to automatically scan detector strips to identify electrical issues. Both systems can provide accurate information but at the same time they can be easily operated by students.

  5. Vadose zone characterization project at the Hanford Tank Farms: BY Tank Farm report

    Kos, S.E.

    1997-02-01

    The US Department of Energy Grand Junction Office (GJO) was tasked by the DOE Richland Operations Office (DOE-RL) to perform a baseline characterization of the contamination distributed in the vadoze zone sediment beneath and around the single-shell tanks (SSTs) at the Hanford Site. The intent of this characterization is to determine the nature and extent of the contamination, to identify contamination sources, and to develop a baseline of the contamination distribution that will permit future data comparisons. This characterization work also allows an initial assessment of the impacts of the vadose zone contamination as required by the Resource Conservation and Recovery Act (RCRA). This characterization project involves acquiring information about the vadose zone contamination with borehole geophysical logging methods and documenting that information in a series of reports. Data from boreholes surrounding each tank are compiled into individual Tank Summary Data Reports. The data from each tank farm are then compiled and summarized in a Tank Farm Report. This document is the Tank Farm Report for the BY Tank Farm

  6. Vadose zone characterization project at the Hanford Tank Farms: BY Tank Farm report

    Kos, S.E.

    1997-02-01

    The US Department of Energy Grand Junction Office (GJO) was tasked by the DOE Richland Operations Office (DOE-RL) to perform a baseline characterization of the contamination distributed in the vadoze zone sediment beneath and around the single-shell tanks (SSTs) at the Hanford Site. The intent of this characterization is to determine the nature and extent of the contamination, to identify contamination sources, and to develop a baseline of the contamination distribution that will permit future data comparisons. This characterization work also allows an initial assessment of the impacts of the vadose zone contamination as required by the Resource Conservation and Recovery Act (RCRA). This characterization project involves acquiring information about the vadose zone contamination with borehole geophysical logging methods and documenting that information in a series of reports. Data from boreholes surrounding each tank are compiled into individual Tank Summary Data Reports. The data from each tank farm are then compiled and summarized in a Tank Farm Report. This document is the Tank Farm Report for the BY Tank Farm.

  7. Characterization of Boerhavia diffusa L. mutant lines by RAPD and isozyme, selected for agronomically valuable traits

    Shukla, N.; Sangwan, N.S.; Misra, H.O.; Sangwan, R.S.

    2004-01-01

    Boerhavia diffusa is a medicinally important plant and finds extensive uses in traditional herbal drug preparations. For the development of improved varieties in terms of superior yield and quality of herb/root of B. diffusa, mutation breeding was attempted. Mutants generated by physical and chemical mutagenic treatments were screened for yield and quality parameters of the root/herb up to three consecutive generations. The selected-screened lines generated by physical and chemical mutagenic treatments on two selected genotypes I and II were molecularly analyzed using eight isozymes and eleven RAPD primers producing good amplification. Mutants from BD10 (selected genotype I) were distinct, while, in case of BD22 (selected genotype II), only one mutant BDMu7 was recorded distinct by isozyme analysis. The wild mutant (BDMu16, with maximum height and mouve coloured flower) was distinct in RAPD banding pattern. Isozymes differentiated the mutants from their respective controls, whereas RAPD differentiated the mutants and controls and also distinguished the mutants. The RAPD analysis was found to be better suited than isozymes for detecting genetic differences among controls and their mutants. However, both RAPD and isozyme analyses gave similar patterns of genetic relationships [it

  8. Molecular and morpho-anatomical characterization of some Egyptian durum wheat cultivars/lines

    Saleh, O.M.; Hamiedeldin, N.; Khafaga, A.

    2016-01-01

    Grains of eight durum wheat cultivars were tested for identification of genetic relationship among molecular, anatomical and morphological levels. On the molecular level, two techniques have been used; Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR). Amplification of RAPD primers showed different numbers of fragments ranged from six to thirteen fragments. Percentage of polymorphism ranged from 0% to 100%. The highest similarity value recorded was 91%, while the lowest similarity value was 69%. Amplification of ISSR primers showed different numbers of fragments ranged from six to twelve fragments. The highest similarity value recorded was 91%, while the lowest similarity value was 68%. The grain's coat morphology was reticulated in all taxa. There were variations with regard to the alignment and the shape of network and architecture of interspaces enclosed by raised line. Reticulate surface patterns appeared some variations ranged from weakly reticulate such as G 413 to strongly reticulate such as G 203. Stem cuticles of all cultivars were thick except cultivar; Benisweif 1. For leaf anatomy, all cultivars had epidermis composed of one layer of thick wall cells except cultivars; G 203 and Benisweif 1. (author)

  9. Characterization of DNA repair phenotypes of Xeroderma pigmentosum cell lines by a paralleled in vitro test

    Raffin, A.L.

    2009-06-01

    DNA is constantly damaged modifying the genetic information for which it encodes. Several cellular mechanisms as the Base Excision Repair (BER) and the Nucleotide Excision Repair (NER) allow recovering the right DNA sequence. The Xeroderma pigmentosum is a disease characterised by a deficiency in the NER pathway. The aim of this study was to propose an efficient and fast test for the diagnosis of this disease as an alternative to the currently available UDS test. DNA repair activities of XP cell lines were quantified using in vitro miniaturized and paralleled tests in order to establish DNA repair phenotypes of XPA and XPC deficient cells. The main advantage of the tests used in this study is the simultaneous measurement of excision or excision synthesis (ES) of several lesions by only one cellular extract. We showed on one hand that the relative ES of the different lesions depend strongly on the protein concentration of the nuclear extract tested. Working at high protein concentration allowed discriminating the XP phenotype versus the control one, whereas it was impossible under a certain concentration's threshold. On the other hand, while the UVB irradiation of control cells stimulated their repair activities, this effect was not observed in XP cells. This study brings new information on the XPA and XPC protein roles during BER and NER and underlines the complexity of the regulations of DNA repair processes. (author)

  10. Formation of brown lines in paper: characterization of cellulose degradation at the wet-dry interface.

    Souguir, Zied; Dupont, Anne-Laurence; de la Rie, E René

    2008-09-01

    Brown lines were generated at the wet-dry interface on Whatman paper No. 1 by suspending the sheet vertically in deionized water. Formic acid and acetic acid were quantified in three areas of the paper defined by the wet-dry boundary (above, below, and at the tideline) using capillary zone electrophoresis with indirect UV detection. Their concentration increased upon accelerated aging of the paper and was highest in the tideline. The hydroperoxides have been quantified using reverse phase high performance liquid chromatography with UV detection based on the determination of triphenylphosphine oxide produced from the reaction with triphenylphosphine, and their highest concentration was found in the tideline as well. For the first time, it was shown that various types of hydroperoxides were present, water-soluble and non-water-soluble, most probably in part hydroperoxide functionalized cellulose. After accelerated aging, a significant increase in hydroperoxide concentration was found in all the paper areas. The molar masses of cellulose determined using size-exclusion chromatography with multiangle light scattering detection showed that, upon aging, cellulose degraded significantly more in the tideline area than in the other areas of the paper. The area below the tideline was more degraded than the area above. A kinetic study of the degradation of cellulose allowed determining the constants for glycosidic bond breaking in each of the areas of the paper.

  11. Characterizing Neutron Diagnostics on the nTOF Line at SUNY Geneseo

    Harrison, Hannah; Seppala, Hannah; Visca, Hannah; Wakwella, Praveen; Fletcher, Kurt; Padalino, Stephen; Forrest, Chad; Regan, Sean; Sangster, Craig

    2016-10-01

    Charged particle beams from SUNY Geneseo's 1.7 MV Tandem Pelletron Accelerator induce nuclear reactions that emit neutrons ranging from 0.5 to 17.9 MeV via 2H(d,n)3He and 11B(d,n)12C. This adjustable neutron source can be used to calibrate ICF and HEDP neutron scintillators for ICF diagnostics. However, gamma rays and muons, which are often present during an accelerator-based calibration, are difficult to differentiate from neutron signals in scintillators. To mitigate this problem, a new neutron time-of-flight (nTOF) line has been constructed. The nTOF timing is measured using the associated particle technique. A charged particle produced by the nuclear reaction serves as a start signal, while its associated neutron is the stop signal. Each reaction is analyzed event-by-event to determine whether the scintillator signal was generated by a neutron, gamma or muon. Using this nTOF technique, the neutron response for different scintillation detectors can be determined. Funded in part by a LLE contract through the DOE.

  12. Characterizing ICF Neutron Diagnostics on the nTOF line at SUNY Geneseo

    Simone, Angela; Padalino, Stephen; Turner, Ethan; Ginnane, Mary Kate; Dubois, Natalie; Fletcher, Kurtis; Giordano, Michael; Lawson-Keister, Patrick; Harrison, Hannah; Visca, Hannah; Sangster, Craig; Regan, Sean

    2014-10-01

    Charged particle beams from the Geneseo 1.7 MV tandem Pelletron accelerator produce nuclear reactions that emit neutrons in the range of 0.5 to 17.9 MeV via the d(d,n)3He and 11B(d,n)12C reactions. The neutron energy and flux can be adjusted by controlling the accelerator beam current and potential. This adjustable neutron source makes it possible to calibrate ICF and HEDP neutron scintillator diagnostics. However, gamma rays which are often present during an accelerator-based calibration are difficult to differentiate from neutron signals in scintillators. To identify neutrons from gamma rays and to determine their energy, a permanent neutron time-of-flight (nTOF) line is being constructed. By detecting the scintillator signal in coincidence with an associated charged particle (ACP) produced in the reaction, the identity of the neutron can be known and its energy determined by time of flight. Using a 100% efficient surface barrier detector to count the ACPs, the absolute efficiency of the scintillator as a function of neutron energy can be determined. This is done by determining the ratio of the ACP counts in the singles spectrum to coincidence counts for matched solid angles of the SBD and scintillator. Funded in part by a LLE contract through the DOE.

  13. Characterization of Ebola virus entry by using pseudotyped viruses: identification of receptor-deficient cell lines.

    Wool-Lewis, R J; Bates, P

    1998-04-01

    Studies analyzing Ebola virus replication have been severely hampered by the extreme pathogenicity of this virus. To permit analysis of the host range and function of the Ebola virus glycoprotein (Ebo-GP), we have developed a system for pseudotyping these glycoproteins into murine leukemia virus (MLV). This pseudotyped virus, MLV(Ebola), can be readily concentrated to titers which exceed 5 x 10(6) infectious units/ml and is effectively neutralized by antibodies specific for Ebo-GP. Analysis of MLV(Ebola) infection revealed that the host range conferred by Ebo-GP is very broad, extending to cells of a variety of species. Notably, all lymphoid cell lines tested were completely resistant to infection; we speculate that this is due to the absence of a cellular receptor for Ebo-GP on B and T cells. The generation of high-titer MLV(Ebola) pseudotypes will be useful for the analysis of immune responses to Ebola virus infection, development of neutralizing antibodies, analysis of glycoprotein function, and isolation of the cellular receptor(s) for the Ebola virus.

  14. Molecular characterization of irinotecan (SN-38) resistant human breast cancer cell lines

    Jandu, Haatisha; Aluzaite, Kristina; Fogh, Louise

    2016-01-01

    Background: Studies in taxane and/or anthracycline refractory metastatic breast cancer (mBC) patients have shown approximately 30 % response rates to irinotecan. Hence, a significant number of patients will experience irinotecan-induced side effects without obtaining any benefit. The aim of this ......Background: Studies in taxane and/or anthracycline refractory metastatic breast cancer (mBC) patients have shown approximately 30 % response rates to irinotecan. Hence, a significant number of patients will experience irinotecan-induced side effects without obtaining any benefit. The aim...... or an initial high dose of SN-38 (the active metabolite of irinotecan), respectively. The resistant cell lines were analyzed for cross-resistance to other anti-cancer drugs, global gene expression, growth rates, TOP1 and TOP2A gene copy numbers and protein expression, and inhibition of the breast cancer...... of the BCRP in breast cancer patients scheduled for irinotecan treatment. Moreover, LMP400 should be tested in a clinical setting in breast cancer patients with resistance to irinotecan....

  15. Project W-314 specific test and evaluation plan for SN-635 transfer line (241-AY-01A to 241-AY-02A) and SN-633 transfer line tie in

    Hays, W.H.

    1998-01-01

    This Specific Test and Evaluation Plan (STEP) defines the test and evaluation activities encompassing the installation of the SN-635 transfer line for the W-314 Project. The purpose of this Specific Test and Evaluation Plan (STEP) is to provide a detailed written plan for the systematic testing of modifications made by the addition of the SN-635 transfer line and the tie in of SN-633 to the AY-02A pit by the W-314 Project. The STEP develops the outline for test procedures that verify the system's performance to the established Project design criteria. The STEP is a lower tier document based on the W-314 Test and Evaluation Plan (TEP)

  16. El Paso Electric Company Diablo Substation to the US-Mexico border 115kV transmission line project. Final Environmental Assessment

    1992-04-01

    This Environmental Assessment documents the analysis of alternative corridors for development and operation of a proposed 115 kilovolt transmission line using private lands and transporting power to the US-Mexico international border. The project will require (1) an amendment to El Paso Electric Company`s existing export authorization to transfer power across this border, and (2) a Presidential Permit for construction of the transmission line. The project would be located in Dona Ana county in southern New Mexico, approximately five miles west of El Paso, Texas. The alternative corridors, specific locations within those corridors, and structure types are identified and analyzed in the environmental studies.

  17. Groups of homeomorphisms of the line. Criteria for the existence of invariant and projectively invariant measures in terms of the commutator subgroup

    Beklaryan, L A [Central Economics and Mathematics Institute, RAS, Moscow (Russian Federation)

    2014-12-31

    Existence criteria for invariant and projectively invariant measures are obtained for a group G of homeomorphisms of the line. These criteria are formulated in terms of the commutator subgroup [G,G]. For the special (but very important) case of groups of homeomorphisms of the line containing a freely acting element we obtain a criterion for the existence of a projectively invariant measure in the form of the absence of a special subgroup with two generators in which one of the generating elements is a freely acting element. Bibliography: 20 titles.

  18. In vitro phenotypic, genomic and proteomic characterization of a cytokine-resistant murine β-TC3 cell line.

    Antonina Coppola

    Full Text Available Type 1 diabetes mellitus (T1DM is caused by the selective destruction of insulin-producing β-cells. This process is mediated by cells of the immune system through release of nitric oxide, free radicals and pro-inflammatory cytokines, which induce a complex network of intracellular signalling cascades, eventually affecting the expression of genes involved in β-cell survival.The aim of our study was to investigate possible mechanisms of resistance to cytokine-induced β-cell death. To this purpose, we created a cytokine-resistant β-cell line (β-TC3R by chronically treating the β-TC3 murine insulinoma cell line with IL-1β + IFN-γ. β-TC3R cells exhibited higher proliferation rate and resistance to cytokine-mediated cell death in comparison to the parental line. Interestingly, they maintained expression of β-cell specific markers, such as PDX1, NKX6.1, GLUT2 and insulin. The analysis of the secretory function showed that β-TC3R cells have impaired glucose-induced c-peptide release, which however was only moderately reduced after incubation with KCl and tolbutamide. Gene expression analysis showed that β-TC3R cells were characterized by downregulation of IL-1β and IFN-γ receptors and upregulation of SOCS3, the classical negative regulator of cytokines signaling. Comparative proteomic analysis showed specific upregulation of 35 proteins, mainly involved in cell death, stress response and folding. Among them, SUMO4, a negative feedback regulator in NF-kB and JAK/STAT signaling pathways, resulted hyper-expressed. Silencing of SUMO4 was able to restore sensitivity to cytokine-induced cell death in β-TC3R cells, suggesting it may play a key role in acquired cytokine resistance by blocking JAK/STAT and NF-kB lethal signaling.In conclusion, our study represents the first extensive proteomic characterization of a murine cytokine-resistant β-cell line, which might represent a useful tool for studying the mechanisms involved in resistance to

  19. Characterization of the central neural projections to brown, white, and beige adipose tissue.

    Wiedmann, Nicole M; Stefanidis, Aneta; Oldfield, Brian J

    2017-11-01

    The functional recruitment of classic brown adipose tissue (BAT) and inducible brown-like or beige fat is, to a large extent, dependent on intact sympathetic neural input. Whereas the central neural circuits directed specifically to BAT or white adipose tissue (WAT) are well established, there is only a developing insight into the nature of neural inputs common to both fat types. Moreover, there is no clear view of the specific central and peripheral innervation of the browned component of WAT: beige fat. The objective of the present study is to examine the neural input to both BAT and WAT in the same animal and, by exposing different cohorts of rats to either thermoneutral or cold conditions, define changes in central neural organization that will ensure that beige fat is appropriately recruited and modulated after browning of inguinal WAT (iWAT). At thermoneutrality, injection of the neurotropic (pseudorabies) viruses into BAT and WAT demonstrates that there are dedicated axonal projections, as well as collateral axonal branches of command neurons projecting to both types of fat. After cold exposure, central neural circuits directed to iWAT showed evidence of reorganization with a greater representation of command neurons projecting to both brown and beiged WAT in hypothalamic (paraventricular nucleus and lateral hypothalamus) and brainstem (raphe pallidus and locus coeruleus) sites. This shift was driven by a greater number of supraspinal neurons projecting to iWAT under cold conditions. These data provide evidence for a reorganization of the nervous system at the level of neural connectivity following browning of WAT.-Wiedmann, N. M., Stefanidis, A., Oldfield, B. J. Characterization of the central neural projections to brown, white, and beige adipose tissue. © FASEB.

  20. A Guide to On-line Geological Information and Publications for Use in GSHP Site Characterization

    Rafferty, K

    2000-03-01

    One of the first steps in the consideration of a GSHP system is a characterization of the site in terms of geology and groundwater availability. Information concerning aquifer (or aquifers) available at the site, their ability to produce water, depth to water, geology, depth to bedrock and the nature of the soil and rock (hydraulic and thermal properties) are key issues. This information guides the designer in the selection of the type of GSHP system to be used and in the design of the system. The ground source industry has not taken full advantage of available geological information resources in the past. This document is an effort to introduce GSHP designers to some of these information sources and the nature of the data that is available. A special emphasis has been placed on Internet based resources operated by government agencies--primarily the USGS and state geological surveys. The following section provides some background information on the maps and other information sources in general. This is followed by summaries of information available for the most active GSHP states.

  1. Canine osteosarcoma cell lines contain stem-like cancer cells: biological and pharmacological characterization.

    Gatti, Monica; Wurth, Roberto; Vito, Guendalina; Pattarozzi, Alessandra; Campanella, Chiara; Thellung, Stefano; Maniscalco, Lorella; De Maria, Raffaella; Villa, Valentina; Corsaro, Alessandro; Nizzari, Mario; Bajetto, Adriana; Ratto, Alessandra; Ferrari, Angelo; Barbieri, Federica; Florio, Tullio

    2016-05-01

    Cancer stem cells (CSCs) represent a small subpopulation of cells responsible for tumor formation and progression, drug resistance, tumor recurrence and metastasization. CSCs have been identified in many human tumors including osteosarcoma (OSA). CSC distinctive properties are the expression of stem cell markers, sustained growth, self-renewal and tumorigenicity. Here we report the isolation of stem-like cells from two canine OSA cultures, characterized by self-renewal, evaluated by sphere formation ability, differential marker expression, and in vitro proliferation when cultured in a medium containing EGF and bFGF. Current therapies for OSA increased survival time, but prognosis remains poor, due to the development of drug resistance and metastases. Chemotherapy shrinks the tumor mass but CSCs remain unaffected, leading to tumor recurrence. Metformin, a drug for type 2 diabetes, has been shown to possess antitumor properties affecting CSC survival in different human and animal cancers. Here we show that metformin has a significant antiproliferative effect on canine OSA stem-like cells, validating this in vitro model for further pre-clinical drug evaluations. In conclusion, our results demonstrate the feasibility of obtaining CSC-enriched cultures from primary canine OSA cells as a promising model for biological and pharmacological studies of canine and human OSAs.

  2. Characterization of endothelin receptors on a human neuroblastoma cell line: evidence for the ETA subtype.

    Wilkes, L C; Boarder, M R

    1991-11-01

    1. Specific binding sites for synthetic endothelin (ET) isoforms were studied on intact cells of the SK-N-MC cell line, derived from a human neuroblastoma. 2. [125I]-ET-1 (2.5 x 10(-11) M) specifically bound to a single class of binding sites on these cells (Hill coefficient of 1.06 +/- 0.04, n = 3) with an apparent Kd of 1.4 +/- 0.3 x 10(-9) M and a Bmax of 3.1 +/- 1.0 pmol mg-1 protein. [125I]-ET-3 (2.5 x 10(-11) M), did not specifically bind to SK-N-MC cells. 3. The binding of [125I]-ET-1 was competitively inhibited by other ET isoforms, the order of potency being ET-1 greater than sarafotoxin S6b greater than ET-3. 4. Association of 1 nM [125I]-ET-1 at 37 degrees C reached apparent equilibrium at 60-80 min, with half-maximal binding being achieved at 12 min. 5. Dissociation was measured after both 10 min and 60 min of association with 64% and 30% respectively of specifically bound [125I]-ET-1 dissociating. The actual amounts of [125I]-ET-1 dissociated were similar in both cases. 6. Incubation of [125I]-ET-3 with SK-N-MC cells at 37 degrees C for 60 min did not result in significant degradation of this peptide. However, [125I]-ET-1 was broken down by incubation with SK-N-MC cells, the pattern of degradation of dissociable [125I]-ET-1 (and that found in the supernatant) being different from that of non-dissociable [125I]-ET-1. 7. ET-1 concentration-dependently induced an increase in total inositol phosphate accumulation in subconfluent (but not in confluent) cultures of SK-N-MC cells (EC50 = 6.43 +/- 1.9 x 1010M). ET-3 was without effect. 8. These results show that ET-1 specifically binds to SK-N-MC cells with the characteristics of an ETA receptor. Our earlier finding that adrenal chromaffin cells express an ETB receptor indicates the existence of multiple ET receptor types on neuronal cells.

  3. The Space Geodesy Project and Radio Frequency Interference Characterization and Mitigation

    Lawrence, Hilliard M.; Beaudoin, C.; Corey, B. E.; Tourain, C. L.; Petrachenko, B.; Dickey, John

    2013-01-01

    The Space Geodesy Project (SGP) development by NASA is an effort to co-locate the four international geodetic techniques Satellite Laser Ranging (SLR) and Lunar Laser Ranging (LLR), Very Long Baseline Interferometry (VLBI), Global Navigation Satellite System (GNSS), and Doppler Orbitography and Radiopositioning Integrated by Satellite (DORIS) into one tightly referenced campus and coordinated reference frame analysis. The SGP requirement locates these stations within a small area to maintain line-of-sight and frequent automated survey known as the vector tie system. This causes a direct conflict with the new broadband VLBI technique. Broadband means 2-14 GHz, and RFI susceptibility at -80 dBW or higher due to sensitive RF components in the front end of the radio receiver.

  4. Standard characterization of phosphate rock samples from the FAO/IAEA phosphate project

    Binh, Truong; Zapata, F.

    2002-01-01

    Phosphate rocks (PR) are phosphate-bearing minerals that vary widely in their inherent characteristics and consequently their agronomic potential. In the framework of a FAO/IAEA networked research project, the evaluation of the agronomic effectiveness of natural and modified PR products under a variety of soil climate and crop management conditions was carried out. The characterization of phosphate rocks is the first and essential step in evaluating their suitability for direct application. If several PR sources are utilized, standardized methods should be used for comparison purposes to determine their agronomic potential. This paper describes the standard characterization of phosphate rock products utilized in the project, in particular the mineralogical and crystallographic analyses, physical analyses, chemical composition and solubility in conventional reagents. A total of 28 phosphate rock samples from 15 countries were collected and analyzed in specialized laboratories. The data on mineralogy, chemical composition and solubility in conventional reagents are closely interrelated. An arbitrary classification of the reactivity of the PR samples was made based on the solubility indices in conventional reagents. On another hand, the results of the crystallographic parameters, calculated indices of absolute solubility, specific surface and porosity reflect the variability of the physical state and the sample pre-conditioning treatment of the analyzed products. A proper characterization of phosphate rock samples should provide the maximum of basic information that can be obtained in a cost-effective manner in normal chemical laboratories. Based on the results of this characterization, the following determinations are recommended: a description of the sample, major elemental (total P, Ca, Mg) composition, solubility in conventional reagents (neutral ammonium citrate, citric and formic acid) and particle size analysis. The classification of PR samples for direct

  5. A nanocomplex of Cu(II) with theophylline drug; synthesis, characterization, and anticancer activity against K562 cell line

    Sahlabadi, Maryam; Daryanavard, Marzieh; Hadadzadeh, Hassan; Amirghofran, Zahra

    2018-03-01

    A new mononuclear of copper (II), [Cu(theophylline)2(H2O)3]·2H2O, has been synthesized by reaction of theophylline (1,3-dimethyl-7H-purine-2,6-dione) with copper (II) nitrate in water. Further, its nanocomplex has been prepared through the three different methods including sonication, grinding, and a combination thereof, sonication-grinding. The prepared nanocomplex was characterized using different techniques including FT-IR, UV-Vis, X-ray diffraction (XRD) analysis, and field-emission scanning electron microscopy (FE-SEM). Moreover, the anticancer activity of the precursor complex, nanocomplex, free theophylline ligand, and the starting copper salt (Cu(NO3)2·3H2O) was investigated against the K562 cell line. The results show that the nanocomplex is an effective nano metal-based anticancer agent with IC50 = 11.7 μM.

  6. Characterization of fibroblast growth factor receptor 2 overexpression in the human breast cancer cell line SUM-52PE

    Tannheimer, Stacey L; Rehemtulla, Alnawaz; Ethier, Stephen P

    2000-01-01

    three times more transformed foci in soft agar than C1 variants (both IIIb), whereas full length FGFR2 and FGFR1 (both IIIc variants) showed no transforming activity [4]. Previous studies [5,6] have found amplification and overexpression of FGFR2 in 5-10% of primary breast cancer specimens. A recent study [7] done using a tissue array consisting of 372 primary breast cancer specimens found a 5% incidence of FGFR2 amplification. To our knowledge, none of the HBC cell lines studied thus far have an FGFR2 gene amplification, although overexpression of FGFR2 message and protein has been documented for some breast cancer cell lines [6,8,9]. SUM-52PE is a breast cancer cell line previously isolated in our laboratory that grows under serum-free and epidermal growth factor-free conditions, has high levels of tyrosine-phosphorylated membrane proteins, and has the capacity to invade and grow under anchorage-independent conditions [10,11,12]. This cell line exhibits all of the important hallmarks of transformed, highly malignant cells. Therefore, SUM-52PE was used as a model to study the diversity of FGFR2 expression in a breast cancer cell line that has true amplification and overexpression of FGFR2. This study was conducted to examine the degree of FGFR2 amplification and overexpression in the breast cancer cell line SUM-52PE. Subsequent sequencing and characterization of individual FGFR2 variants cloned from the SUM-52PE cell line was completed to determine the complexity of FGFR2 alternative splicing in the context of a highly metastatic breast cancer cell line. Southern, Northern and Western blot analyses were done in order to determine the degree of FGFR2 amplification and overexpression in the breast cancer cell line SUM-52PE. Individual FGFR2 variants were cloned out of SUM-52PE using FGFR2-specific primers in a reverse transcription (RT) polymerase chain reaction (PCR). FGFR2 cDNAs were characterized by restriction fragment analysis, sequencing and transient transfection

  7. Establishment of a human cell line stably overexpressing mouse Nip45 and characterization of Nip45 subcellular localization

    Hashiguchi, Kohtaro; Ozaki, Masumi [Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto (Japan); Kuraoka, Isao [Biological Chemistry Group, Division of Chemistry, Graduate School of Engineering Science, Osaka University, Osaka (Japan); Saitoh, Hisato, E-mail: hisa@kumamoto-u.ac.jp [Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto (Japan); Department of New Frontier Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto (Japan); Global COE (Centers of Excellence) Program, Global Initiative Center for Pulsed Power Engineering, Kumamoto University, Kumamoto (Japan)

    2013-01-04

    Highlights: Black-Right-Pointing-Pointer A human cell line expressing a mouse Nip45 has facilitated Nip45 analysis. Black-Right-Pointing-Pointer Nip45 does not effectively inhibit polySUMOylation in vivo. Black-Right-Pointing-Pointer Nip45 interacts directly with SUMO and SUMO chains. Black-Right-Pointing-Pointer Nip45 accumulates at PML bodies in response to proteasome inhibition. -- Abstract: The nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 interacting protein, Nfatc2ip (Nip45), has been implicated as a crucial coordinator of the immune response and of cellular differentiation in humans and mice, and contains SUMO-like domains in its C-terminal region. However, the significance of its N-terminal region and its correlation to the SUMO modification pathway remain largely uncharacterized. In this study, a human cultured cell line was established, in which FLAG-tagged mouse Nip45 (FLAG-mNip45) was stably overexpressed. Under standard, non-stressful conditions, we detected FLAG-mNip45 diffusely distributed in the nucleus. Intriguingly, proteasome inhibition by MG132 caused FLAG-mNip45, together with SUMOylated proteins, to localize in nuclear domains associated with promyelocytic leukemia protein. Finally, using an in vitro binding assay, we showed interaction of the N-terminal region of mNip45 with both free SUMO-3 and SUMO-3 chains, indicating that Nip45 may, in part, exert its function via interaction with SUMO/SUMOylated proteins. Taken together, our study provides novel information on a poorly characterized mammalian protein and suggests that our newly established cell line will be useful for elucidating the physiological role of Nip45.

  8. Evaluation of measurement reproducibility using the standard-sites data, 1994 Fernald field characterization demonstration project

    Rautman, C.A.

    1996-02-01

    The US Department of Energy conducted the 1994 Fernald (Ohio) field characterization demonstration project to evaluate the performance of a group of both industry-standard and proposed alternative technologies in describing the nature and extent of uranium contamination in surficial soils. Detector stability and measurement reproducibility under actual operating conditions encountered in the field is critical to establishing the credibility of the proposed alternative characterization methods. Comparability of measured uranium activities to those reported by conventional, US Environmental Protection Agency (EPA)-certified laboratory methods is also required. The eleven (11) technologies demonstrated included (1) EPA-standard soil sampling and laboratory mass-spectroscopy analyses, and currently-accepted field-screening techniques using (2) sodium-iodide scintillometers, (3) FIDLER low-energy scintillometers, and (4) a field-portable x-ray fluorescence spectrometer. Proposed advanced characterization techniques included (5) alpha-track detectors, (6) a high-energy beta scintillometer, (7) electret ionization chambers, (8) and (9) a high-resolution gamma-ray spectrometer in two different configurations, (10) a field-adapted laser ablation-inductively coupled plasma-atomic emission spectroscopy (ICP-AES) technique, and (11) a long-range alpha detector. Measurement reproducibility and the accuracy of each method were tested by acquiring numerous replicate measurements of total uranium activity at each of two ''standard sites'' located within the main field demonstration area. Meteorological variables including temperature, relative humidity. and 24-hour rainfall quantities were also recorded in conjunction with the standard-sites measurements

  9. The socio-economic base line survey; first chapter of the handbook under preparation: "Managing farmers: a handbook for working with farmers in irrigation and drainage projects"

    Schrevel, A.

    2002-01-01

    The text The socio-economic base line survey is the first chapter of a book under preparation meant to instruct senior staff of irrigation and drainage projects on techniques to work with farmers. It informs the reader of best practices to set up and execute a socio-economic baseline survey. The

  10. Plasma flow switch characterization for the Los Alamos Foil Implosion Project

    Bowers, R.L.; Brownell, J.H.; Greene, A.E.; Peterson, D.L.

    1990-01-01

    The next system design under consideration for the Los Alamos Foil Implosion Project is projected to deliver tens of mega-amperes of electrical current produced by high-explosive driven flux compression generators on a time scale of about one microsecond to a load foil. The use of such generators, with time scales of order several tenths of a millisecond, leads to considerable pulse shaping problems. Previously it was noted that a commutating switch might serve as an efficient alternative to a closing switch in transferring current from a coaxial transmission line to a cylindrically imploding load. Research at the Air Force Weapons Laboratory (AFWL) has met with considerable success in efficiently transferring currents of order 10 MA to an imploding liner using the plasma flow switch concept (PFS). Besides efficiently transferring current, the plasma flow switch protects the load region from high voltages generated by an opening switch until the current is present to provide magnetic insulation. For these reasons, a PFS is being investigated as the final pulse shaping step in the design. A series of capacitor bank experiments is also being fielded to help investigate physics issues and to benchmark the codes

  11. Characterization of a novel telomerase-immortalized human endometrial stromal cell line, St-T1b

    Brosens Jan J

    2009-07-01

    Full Text Available Abstract Background Coordinated differentiation of the endometrial compartments in the second half of the menstrual cycle is a prerequisite for the establishment of pregnancy. Endometrial stromal cells (ESC decidualize under the influence of ovarian progesterone to accommodate implantation of the blastocyst and support establishment of the placenta. Studies into the mechanisms of decidualization are often hampered by the lack of primary ESC. Here we describe a novel immortalized human ESC line. Methods Primary ESC were immortalized by the transduction of telomerase. The resultant cell line, termed St-T1b, was characterized for its morphological and biochemical properties by immunocytochemistry, RT-PCR and immunoblotting. Its progestational response was tested using progesterone and medroxyprogesterone acetate with and without 8-Br-cAMP, an established inducer of decidualization in vitro. Results St-T1b were positive for the fibroblast markers vimentin and CD90 and negative for the epithelial marker cytokeratin-7. They acquired a decidual phenotype indistinguishable from primary ESC in response to cAMP stimulation. The decidual response was characterized by transcriptional activation of marker genes, such as PRL, IGFBP1, and FOXO1, and enhanced protein levels of the tumor suppressor p53 and the metastasis suppressor KAI1 (CD82. Progestins alone had no effect on St-T1b cells, but medroxyprogesterone acetate greatly enhanced the cAMP-stimulated expression of IGFBP-1 after 3 and 7 days. Progesterone, albeit more weakly, also augmented the cAMP-induced IGFBP-1 production but only after 7 days of treatment. The cell line remained stable in continuous culture for more than 150 passages. Conclusion St-T1b express the appropriate phenotypic ESC markers and their decidual response closely mimics that of primary cultures. Decidualization is efficiently induced by cAMP analog and enhanced by medroxyprogesterone acetate, and, to a lesser extent, by natural

  12. In vitro identification and characterization of CD133(pos cancer stem-like cells in anaplastic thyroid carcinoma cell lines.

    Giovanni Zito

    Full Text Available Recent publications suggest that neoplastic initiation and growth are dependent on a small subset of cells, termed cancer stem cells (CSCs. Anaplastic Thyroid Carcinoma (ATC is a very aggressive solid tumor with poor prognosis, characterized by high dedifferentiation. The existence of CSCs might account for the heterogeneity of ATC lesions. CD133 has been identified as a stem cell marker for normal and cancerous tissues, although its biological function remains unknown.ATC cell lines ARO, KAT-4, KAT-18 and FRO were analyzed for CD133 expression. Flow cytometry showed CD133(pos cells only in ARO and KAT-4 (64+/-9% and 57+/-12%, respectively. These data were confirmed by qRT-PCR and immunocytochemistry. ARO and KAT-4 were also positive for fetal marker oncofetal fibronectin and negative for thyrocyte-specific differentiating markers thyroglobulin, thyroperoxidase and sodium/iodide symporter. Sorted ARO/CD133(pos cells exhibited higher proliferation, self-renewal, colony-forming ability in comparison with ARO/CD133(neg. Furthermore, ARO/CD133(pos showed levels of thyroid transcription factor TTF-1 similar to the fetal thyroid cell line TAD-2, while the expression in ARO/CD133(neg was negligible. The expression of the stem cell marker OCT-4 detected by RT-PCR and flow cytometry was markedly higher in ARO/CD133(pos in comparison to ARO/CD133(neg cells. The stem cell markers c-KIT and THY-1 were negative. Sensitivity to chemotherapy agents was investigated, showing remarkable resistance to chemotherapy-induced apoptosis in ARO/CD133(pos when compared with ARO/CD133(neg cells.We describe CD133(pos cells in ATC cell lines. ARO/CD133(pos cells exhibit stem cell-like features--such as high proliferation, self-renewal ability, expression of OCT-4--and are characterized by higher resistance to chemotherapy. The simultaneous positivity for thyroid specific factor TTF-1 and onfFN suggest they might represent putative thyroid cancer stem-like cells. Our in

  13. Phenotypic and genotypic characterization of Thai isolates of Plasmodium falciparum after an artemisinin resistance containment project.

    Thita, Thunyapit; Jadsri, Pimrat; Thamkhantho, Jarupatr; Ruang-Areerate, Toon; Suwandittakul, Nantana; Sitthichot, Naruemon; Mahotorn, Kittiya; Tan-Ariya, Peerapan; Mungthin, Mathirut

    2018-05-15

    In Thailand, artemisinin-based combination therapy (ACT) has been used to treat uncomplicated falciparum malaria since 1995. Unfortunately, artemisinin resistance has been reported from Thailand and other Southeast Asian countries since 2003. Malarone ® , a combination of atovaquone-proguanil (ATQ-PG), has been used to cease artemisinin pressure in some areas along Thai-Cambodia border, as part of an artemisinin resistance containment project since 2009. This study aimed to determine genotypes and phenotypes of Plasmodium falciparum isolates collected from the Thai-Cambodia border after the artemisinin resistance containment project compared with those collected before. One hundred and nine of P. falciparum isolates collected from Thai-Cambodia border from Chanthaburi and Trat provinces during 1988-2016 were used in this study. Of these, 58 isolates were collected after the containment. These parasite isolates were characterized for in vitro antimalarial sensitivities including chloroquine (CQ), quinine (QN), mefloquine (MQ), piperaquine (PPQ), artesunate (AS), dihydroartemisinin (DHA), ATQ and PG and genetic markers for drug resistance including the Kelch13 (k13), Plasmodium falciparum chloroquine resistance transporter (pfcrt), P. falciparum multidrug resistance 1 (pfmdr1) and cytochrome b (cytb) genes. Mean CQ, QN, MQ, PPQ and AS IC 50 s of the parasite isolates collected from 2009 to 2016 exhibited significantly higher than those of parasites collected before 2009. Approximately 57% exhibited in vitro MQ resistance. Approximately 94% of the isolates collected from 2009 to 2016 contained the pfmdr1 184F allele. Mutations of the k13 gene were detected in approximately 90% of the parasites collected from 2009 to 2016 which were significantly higher than the parasite isolates collected before. No ATQ-resistant genotype and phenotype of P. falciparum were found among the isolates collected after the containment project. Although the containment project had been

  14. Vadose zone characterization project at the Hanford Tank Farms: U Tank Farm Report

    NONE

    1997-05-01

    The U.S. Department of Energy Grand Junction Office (DOE-GJO) was tasked by the DOE Richland Operations Office (DOE-RL) to perform a baseline characterization of the gamma-ray-emitting radionuclides that are distributed in the vadose zone sediments beneath and around the single-shell tanks (SSTs) at the Hanford Site. The intent of this characterization is to determine the nature and extent of the contamination, to identify contamination sources when possible, and to develop a baseline of the contamination distribution that will permit future data comparisons. This characterization work also allows an initial assessment of the impacts of the vadose zone contamination as required by the Resource Conservation and Recovery Act (RCRA). This characterization project involves acquiring information regarding vadose zone contamination with borehole geophysical logging methods and documenting that information in a series of reports. This information is presently limited to detection of gamma-emitting radionuclides from both natural and man-made sources. Data from boreholes surrounding each tank are compiled into individual Tank Summary Data Reports. The data from each tank in a tank farm are then compiled and summarized in a Tank Farm Report. This document is the Tank Farm Report for the U Tank Farm. Logging operations used high-purity germanium detection systems to acquire laboratory-quality assays of the gamma-emitting radionuclides in the sediments around and below the tanks. These assays were acquired in 59 boreholes that surround the U Tank Farm tanks. Logging of all boreholes was completed in December 1995, and the last Tank Summary Data Report for the U Tank Farm was issued in September 1996.

  15. Project W-314 specific test and evaluation plan for SN-633 transfer line (241-AX-B to 241-AY-02A)

    Hays, W.H.

    1998-01-01

    The purpose of this Specific Test and Evaluation Plan (STEP) is to provide a detailed written plan for the systematic testing of modifications made by the addition of the SN-633 transfer line by the W-314 Project. The STEP develops the outline for test procedures that verify the system's performance to the established Project design criteria. The STEP is a lower tier document based on the W-314 Test and Evaluation Plan (TEP). This STEP encompasses all testing activities required to demonstrate compliance to the project design criteria as it relates to the addition of transfer line SN-633. The Project Design Specifications (PDS) identify the specific testing activities required for the Project. Testing includes Validations and Verifications (e.g., Commercial Grade Item Dedication activities), Factory Acceptance Tests (FATs), installation tests and inspections, Construction Acceptance Tests (CATs), Acceptance Test Procedures (ATPs), Pre-Operational Test Procedures (POTPs), and Operational Test Procedures (OTPs). It should be noted that POTPs are not required for testing of the transfer line addition. The STEP will be utilized in conjunction with the TEP for verification and validation

  16. Characterization of Vadose Zone Sediments from C Waste Management Area: Investigation of the C-152 Transfer Line Leak

    Brown, Christopher F.; Serne, R. Jeffrey; Bjornstad, Bruce N.; Valenta, Michelle M.; Lanigan, David C.; Vickerman, Tanya S.; Clayton, Ray E.; Geiszler, Keith N.; Iovin, Cristian; Clayton, Eric T.; Kutnyakov, Igor V.; Baum, Steven R.; Lindberg, Michael J.; Orr, Robert D.

    2008-09-11

    The sodium data was removed due to potential contamination introduced during the acid extraction process. The rest of the text remains unchanged from the original report issued in January 2007. The overall goal of the Tank Farm Vadose Zone Project, led by CH2M HILL Hanford Group, Inc., is to define risks from past and future single-shell tank farm activities at Hanford. To meet this goal, CH2M HILL Hanford Group, Inc., tasked scientists from Pacific Northwest National Laboratory to perform detailed analyses on vadose zone sediments from within waste management area (WMA) C. Specifically, this report contains all the geologic, geochemical, and selected physiochemical characterization data compiled on vadose zone sediment recovered from direct-push samples collected around the site of an unplanned release (UPR), UPR-200-E-82, adjacent to the 241-C-152 Diversion Box located in WMA C.

  17. PERL - European research project on characterization of gaskets for bolted flange connections

    Kockelmann, H.; Hahn, R.

    2004-01-01

    Great progress was observed in the European standardization in the last years in the field of the design of floating type bolted flange connections. New design rules were developed (EN 1591) which include new definitions of gasket characteristics for the calculation of floating type flanged joints. In addition a new gasket testing standard was drafted (prEN 13555) which assures a comprehensive characterization of gaskets for bolted flanged joints. This draft standard contains some new features which were examined and validated within the European research project PERL (Pressure Equipment - Reduction of Leak Rate). The gasket testing strategy laid down in prEN 13555 is presented in this paper. Some testing results highlighten the measuring procedures and the evaluation of the gasket characteristics. (orig.)

  18. Rock mass mechanical property estimations for the Yucca Mountain Site Characterization Project

    Lin, M.; Hardy, M.P.; Bauer, S.J.

    1993-06-01

    Rock mass mechanical properties are important in the design of drifts and ramps. These properties are used in evaluations of the impacts of thermomechanical loading of potential host rock within the Yucca Mountain Site Characterization Project. Representative intact rock and joint mechanical properties were selected for welded and nonwelded tuffs from the currently available data sources. Rock mass qualities were then estimated using both the Norwegian Geotechnical Institute (Q) and Geomechanics Rating (RMR) systems. Rock mass mechanical properties were developed based on estimates of rock mass quality, the current knowledge of intact properties, and fracture/joint characteristics. Empirical relationships developed to correlate the rock mass quality indices and the rock mass mechanical properties were then used to estimate the range of rock mass mechanical properties

  19. Central and Eastern United States (CEUS) Seismic Source Characterization (SSC) for Nuclear Facilities Project

    Kevin J. Coppersmith; Lawrence A. Salomone; Chris W. Fuller; Laura L. Glaser; Kathryn L. Hanson; Ross D. Hartleb; William R. Lettis; Scott C. Lindvall; Stephen M. McDuffie; Robin K. McGuire; Gerry L. Stirewalt; Gabriel R. Toro; Robert R. Youngs; David L. Slayter; Serkan B. Bozkurt; Randolph J. Cumbest; Valentina Montaldo Falero; Roseanne C. Perman' Allison M. Shumway; Frank H. Syms; Martitia (Tish) P. Tuttle

    2012-01-31

    This report describes a new seismic source characterization (SSC) model for the Central and Eastern United States (CEUS). It will replace the Seismic Hazard Methodology for the Central and Eastern United States, EPRI Report NP-4726 (July 1986) and the Seismic Hazard Characterization of 69 Nuclear Plant Sites East of the Rocky Mountains, Lawrence Livermore National Laboratory Model, (Bernreuter et al., 1989). The objective of the CEUS SSC Project is to develop a new seismic source model for the CEUS using a Senior Seismic Hazard Analysis Committee (SSHAC) Level 3 assessment process. The goal of the SSHAC process is to represent the center, body, and range of technically defensible interpretations of the available data, models, and methods. Input to a probabilistic seismic hazard analysis (PSHA) consists of both seismic source characterization and ground motion characterization. These two components are used to calculate probabilistic hazard results (or seismic hazard curves) at a particular site. This report provides a new seismic source model. Results and Findings The product of this report is a regional CEUS SSC model. This model includes consideration of an updated database, full assessment and incorporation of uncertainties, and the range of diverse technical interpretations from the larger technical community. The SSC model will be widely applicable to the entire CEUS, so this project uses a ground motion model that includes generic variations to allow for a range of representative site conditions (deep soil, shallow soil, hard rock). Hazard and sensitivity calculations were conducted at seven test sites representative of different CEUS hazard environments. Challenges and Objectives The regional CEUS SSC model will be of value to readers who are involved in PSHA work, and who wish to use an updated SSC model. This model is based on a comprehensive and traceable process, in accordance with SSHAC guidelines in NUREG/CR-6372, Recommendations for Probabilistic

  20. Advanced reservoir characterization for improved oil recovery in a New Mexico Delaware basin project

    Martin, F.D.; Kendall, R.P.; Whitney, E.M. [Dave Martin and Associates, Inc., Socorro, NM (United States)] [and others

    1997-08-01

    The Nash Draw Brushy Canyon Pool in Eddy County, New Mexico is a field demonstration site in the Department of Energy Class III program. The basic problem at the Nash Draw Pool is the low recovery typically observed in similar Delaware fields. By comparing a control area using standard infill drilling techniques to a pilot area developed using advanced reservoir characterization methods, the goal of the project is to demonstrate that advanced technology can significantly improve oil recovery. During the first year of the project, four new producing wells were drilled, serving as data acquisition wells. Vertical seismic profiles and a 3-D seismic survey were acquired to assist in interwell correlations and facies prediction. Limited surface access at the Nash Draw Pool, caused by proximity of underground potash mining and surface playa lakes, limits development with conventional drilling. Combinations of vertical and horizontal wells combined with selective completions are being evaluated to optimize production performance. Based on the production response of similar Delaware fields, pressure maintenance is a likely requirement at the Nash Draw Pool. A detailed reservoir model of pilot area was developed, and enhanced recovery options, including waterflooding, lean gas, and carbon dioxide injection, are being evaluated.

  1. Fuel Thermo-physical Characterization Project: Evaluation of Models to Calculate Thermal Diffusivity of Layered Composites

    Burkes, Douglas [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Casella, Amanda J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Gardner, Levi D. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Casella, Andrew M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Huber, Tanja K. [Technische Universität München, Munich (Germany); Breitkreutz, Harald [Technische Universität München, Munich (Germany)

    2015-02-11

    The Office of Material Management and Minimization Fuel Thermo-physical Characterization Project at Pacific Northwest National Laboratory (PNNL) is tasked with using PNNL facilities and processes to receive irradiated low enriched uranium-molybdenum fuel plate samples and perform analyses in support of the Office of Material Management and Minimization Reactor Conversion Program. This work is in support of the Fuel Development Pillar that is managed by Idaho National Laboratory. A key portion of the scope associated with this project was to measure the thermal properties of fuel segments harvested from plates that were irradiated in the Advanced Test Reactor. Thermal diffusivity of samples prepared from the fuel segments was measured using laser flash analysis. Two models, one developed by PNNL and the other developed by the Technische Universität München (TUM), were evaluated to extract the thermal diffusivity of the uranium-molybdenum alloy from measurements made on the irradiated, layered composites. The experimental data of the “TC” irradiated fuel segment was evaluated using both models considering a three-layer and five-layer system. Both models are in acceptable agreement with one another and indicate that the zirconium diffusion barrier has a minimal impact on the overall thermal diffusivity of the monolithic U-Mo fuel.

  2. Characterization of apparently balanced chromosomal rearrangements from the developmental genome anatomy project.

    Higgins, Anne W; Alkuraya, Fowzan S; Bosco, Amy F; Brown, Kerry K; Bruns, Gail A P; Donovan, Diana J; Eisenman, Robert; Fan, Yanli; Farra, Chantal G; Ferguson, Heather L; Gusella, James F; Harris, David J; Herrick, Steven R; Kelly, Chantal; Kim, Hyung-Goo; Kishikawa, Shotaro; Korf, Bruce R; Kulkarni, Shashikant; Lally, Eric; Leach, Natalia T; Lemyre, Emma; Lewis, Janine; Ligon, Azra H; Lu, Weining; Maas, Richard L; MacDonald, Marcy E; Moore, Steven D P; Peters, Roxanna E; Quade, Bradley J; Quintero-Rivera, Fabiola; Saadi, Irfan; Shen, Yiping; Shendure, Jay; Williamson, Robin E; Morton, Cynthia C

    2008-03-01

    Apparently balanced chromosomal rearrangements in individuals with major congenital anomalies represent natural experiments of gene disruption and dysregulation. These individuals can be studied to identify novel genes critical in human development and to annotate further the function of known genes. Identification and characterization of these genes is the goal of the Developmental Genome Anatomy Project (DGAP). DGAP is a multidisciplinary effort that leverages the recent advances resulting from the Human Genome Project to increase our understanding of birth defects and the process of human development. Clinically significant phenotypes of individuals enrolled in DGAP are varied and, in most cases, involve multiple organ systems. Study of these individuals' chromosomal rearrangements has resulted in the mapping of 77 breakpoints from 40 chromosomal rearrangements by FISH with BACs and fosmids, array CGH, Southern-blot hybridization, MLPA, RT-PCR, and suppression PCR. Eighteen chromosomal breakpoints have been cloned and sequenced. Unsuspected genomic imbalances and cryptic rearrangements were detected, but less frequently than has been reported previously. Chromosomal rearrangements, both balanced and unbalanced, in individuals with multiple congenital anomalies continue to be a valuable resource for gene discovery and annotation.

  3. Construction and characterization of the detection modules for the Muon Portal Project

    Blancato, A.A.; Bonanno, D.L.; La Rocca, P.; Longhitano, F.; Lo Presti, D.; Parasole, O.; Pugliatti, C.; Riggi, F.; Russo, G.V.; Santagati, G. [Dipartimento di Fisica e Astronomia, Universita di Catania and INFN, Catania, (Italy); Bandieramonte, M.; Billotta, S.; Bonanno, G.; Garozzo, S.; Marano, D.; Riggi, S.; Romeo, G. [INAF, Osservatorio Astrofisico di Catania, (Italy); Fallica, P.G.; Romeo, M. [STMicroelectronics, Catania, (Italy)

    2015-07-01

    The Muon Portal Project is a joint initiative between research and industrial partners, aimed at the construction of a real size detector prototype (6 x 3 x 7 m{sup 3}) for the inspection of containers by the muon scattering technique, devised to search for hidden high-Z fissile materials and provide a full 3D tomography of the interior of the container in a scanning time of the order of minutes. The muon tracking detector is based on a set of 48 detection modules (size 1 m x 3 m), each built with 100 extruded scintillator strips, so as to provide four X-Y detection planes, two placed above and two below the container to be inspected. Two wavelength shifting (WLS) fibres embedded in each strip convey the emitted photons to Silicon Photomultipliers (SiPM) which act as photo-sensors. After a research and development phase, which led to the choice and test of the individual components, the construction of the full size detector has already started. The paper describes the results of the mass characterization of the photo-sensors and the construction and test measurements of the first detection modules of the Project. (authors)

  4. Data Mining Approaches for Genomic Biomarker Development: Applications Using Drug Screening Data from the Cancer Genome Project and the Cancer Cell Line Encyclopedia.

    David G Covell

    Full Text Available Developing reliable biomarkers of tumor cell drug sensitivity and resistance can guide hypothesis-driven basic science research and influence pre-therapy clinical decisions. A popular strategy for developing biomarkers uses characterizations of human tumor samples against a range of cancer drug responses that correlate with genomic change; developed largely from the efforts of the Cancer Cell Line Encyclopedia (CCLE and Sanger Cancer Genome Project (CGP. The purpose of this study is to provide an independent analysis of this data that aims to vet existing and add novel perspectives to biomarker discoveries and applications. Existing and alternative data mining and statistical methods will be used to a evaluate drug responses of compounds with similar mechanism of action (MOA, b examine measures of gene expression (GE, copy number (CN and mutation status (MUT biomarkers, combined with gene set enrichment analysis (GSEA, for hypothesizing biological processes important for drug response, c conduct global comparisons of GE, CN and MUT as biomarkers across all drugs screened in the CGP dataset, and d assess the positive predictive power of CGP-derived GE biomarkers as predictors of drug response in CCLE tumor cells. The perspectives derived from individual and global examinations of GEs, MUTs and CNs confirm existing and reveal unique and shared roles for these biomarkers in tumor cell drug sensitivity and resistance. Applications of CGP-derived genomic biomarkers to predict the drug response of CCLE tumor cells finds a highly significant ROC, with a positive predictive power of 0.78. The results of this study expand the available data mining and analysis methods for genomic biomarker development and provide additional support for using biomarkers to guide hypothesis-driven basic science research and pre-therapy clinical decisions.

  5. YUCCA MOUNTAIN SITE CHARACTERIZATIONS PROJECT TUNNEL BORING MACHINE (TBM) SYSTEM SAFETY ANALYSIS

    1997-01-01

    The purpose of this analysis is to systematically identify and evaluate hazards related to the tunnel boring machine (TBM) used in the Exploratory Studies Facility (ESF) at the Yucca Mountain Site Characterization Project. This process is an integral part of the systems engineering process; whereby safety is considered during planning, design, testing, and construction. Since the TBM is an ''as built'' system, the MandO is conducting the System Safety Analysis during the construction or assembly phase of the TBM. A largely qualitative approach was used since a radiological System Safety Analysis is not required. The risk assessment in this analysis characterizes the accident scenarios associated with the TBM in terms of relative risk and includes recommendations for mitigating all identified risks. The priority for recommending and implementing mitigation control features is: (1) Incorporate measures to reduce risks and hazards into the system/subsystem/component design, (2) add safety features and capabilities to existing designs, and (3) develop procedures and conduct training to increase worker awareness of potential hazards, on methods to reduce exposure to hazards, and on the actions required to avoid accidents or correct hazardous conditions. The scope of this analysis is limited to the TBM during normal operations, excluding hazards occurring during assembly and test of the TBM or maintenance of the TBM equipment

  6. YUCCA MOUNTAIN SITE CHARACTERIZATIONS PROJECT TUNNEL BORING MACHINE (TBM) SYSTEM SAFETY ANALYSIS

    N/A

    1997-02-19

    The purpose of this analysis is to systematically identify and evaluate hazards related to the tunnel boring machine (TBM) used in the Exploratory Studies Facility (ESF) at the Yucca Mountain Site Characterization Project. This process is an integral part of the systems engineering process; whereby safety is considered during planning, design, testing, and construction. Since the TBM is an ''as built'' system, the M&O is conducting the System Safety Analysis during the construction or assembly phase of the TBM. A largely qualitative approach was used since a radiological System Safety Analysis is not required. The risk assessment in this analysis characterizes the accident scenarios associated with the TBM in terms of relative risk and includes recommendations for mitigating all identified risks. The priority for recommending and implementing mitigation control features is: (1) Incorporate measures to reduce risks and hazards into the system/subsystem/component design, (2) add safety features and capabilities to existing designs, and (3) develop procedures and conduct training to increase worker awareness of potential hazards, on methods to reduce exposure to hazards, and on the actions required to avoid accidents or correct hazardous conditions. The scope of this analysis is limited to the TBM during normal operations, excluding hazards occurring during assembly and test of the TBM or maintenance of the TBM equipment.

  7. Cost estimate of high-level radioactive waste containers for the Yucca Mountain Site Characterization Project

    Russell, E.W.; Clarke, W. [Lawrence Livermore National Lab., CA (United States); Domian, H.A. [Babcock and Wilcox Co., Lynchburg, VA (United States); Madson, A.A. [Kaiser Engineers California Corp., Oakland, CA (United States)

    1991-08-01

    This report summarizes the bottoms-up cost estimates for fabrication of high-level radioactive waste disposal containers based on the Site Characterization Plan Conceptual Design (SCP-CD). These estimates were acquired by Babcock and Wilcox (B&S) under sub-contract to Lawrence Livermore National Laboratory (LLNL) for the Yucca Mountain Site Characterization Project (YMP). The estimates were obtained for two leading container candidate materials (Alloy 825 and CDA 715), and from other three vendors who were selected from a list of twenty solicited. Three types of container designs were analyzed that represent containers for spent fuel, and for vitrified high-level waste (HLW). The container internal structures were assumed to be AISI-304 stainless steel in all cases, with an annual production rate of 750 containers. Subjective techniques were used for estimating QA/QC costs based on vendor experience and the specifications derived for the LLNL-YMP Quality Assurance program. In addition, an independent QA/QC analysis is reported which was prepared by Kasier Engineering. Based on the cost estimates developed, LLNL recommends that values of $825K and $62K be used for the 1991 TSLCC for the spent fuel and HLW containers, respectively. These numbers represent the most conservative among the three vendors, and are for the high-nickel anstenitic steel (Alloy 825). 6 refs., 7 figs.

  8. Yucca Mountain Site Characterization Project: Technical data catalog,(quarterly supplement)

    1993-01-01

    The June 1, 1985, Department of Energy (DOE)/Nuclear Regulatory Commission (NRC) Site-Specific Procedural Agreement for Geologic Repository Site Investigation and Characterization Program requires the DOE to develop and maintain a catalog of data which will be updated and provided to the NRC at least quarterly. This catalog is to include a description of the data; the time (date), place, and method of acquisition; and where it may be examined. The Yucca Mountain Site Characterization Project (YMP) Technical Data Catalog is published and distributed in accordance with the requirements of the Site-Specific Agreement. The YMP Technical Data Catalog is a report based on reference information contained in the YMP Automated Technical Data Tracking System (ATDT). The reference information is provided by Participants for data acquired or developed in support of the YMP. The Technical Data Catalog is updated quarterly and published in the month following the end of each quarter. A complete revision to the Catalog is published at the end of each fiscal year

  9. Cost estimate of high-level radioactive waste containers for the Yucca Mountain Site Characterization Project

    Russell, E.W.; Clarke, W.; Domian, H.A.; Madson, A.A.

    1991-08-01

    This report summarizes the bottoms-up cost estimates for fabrication of high-level radioactive waste disposal containers based on the Site Characterization Plan Conceptual Design (SCP-CD). These estimates were acquired by Babcock and Wilcox (B ampersand S) under sub-contract to Lawrence Livermore National Laboratory (LLNL) for the Yucca Mountain Site Characterization Project (YMP). The estimates were obtained for two leading container candidate materials (Alloy 825 and CDA 715), and from other three vendors who were selected from a list of twenty solicited. Three types of container designs were analyzed that represent containers for spent fuel, and for vitrified high-level waste (HLW). The container internal structures were assumed to be AISI-304 stainless steel in all cases, with an annual production rate of 750 containers. Subjective techniques were used for estimating QA/QC costs based on vendor experience and the specifications derived for the LLNL-YMP Quality Assurance program. In addition, an independent QA/QC analysis is reported which was prepared by Kasier Engineering. Based on the cost estimates developed, LLNL recommends that values of $825K and $62K be used for the 1991 TSLCC for the spent fuel and HLW containers, respectively. These numbers represent the most conservative among the three vendors, and are for the high-nickel anstenitic steel (Alloy 825). 6 refs., 7 figs

  10. Vandose Zone Characterization Project at the Hanford Tank Farms: SX Tank Farm Report

    Brodeur, J.R.; Koizumi, C.J.; Bertsch, J.F.

    1996-09-01

    The SX Tank Farm is located in the southwest portion of the 200 West Area of the Hanford Site. This tank farm consists of 15 single-shell tanks (SSTs), each with an individual capacity of 1 million gallons (gal). These tanks currently store high-level nuclear waste that was primarily generated from what was called the oxidation-reduction or open-quotes REDOXclose quotes process at the S-Plant facility. Ten of the 15 tanks are listed in Hanlon as open-quotes assumed leakersclose quotes and are known to have leaked various amounts of high-level radioactive liquid to the vadose zone sediment. The current liquid content of each tank varies, but the liquid from known leaking tanks has been removed to the extent possible. In 1994, the U.S. Department of Energy Richland Office (DOE-RL) requested the DOE Grand Junction Projects Office (GJPO), Grand Junction, Colorado, to perform a baseline characterization of contamination in the vadose zone at all the SST farms with spectral gamma-ray logging of boreholes surrounding the tanks. The SX Tank Farm geophysical logging was completed, and the results of this baseline characterization are presented in this report

  11. Final report on the Background Soil Characterization Project at the Oak Ridge Reservation, Oak Ridge, Tennessee

    Hatmaker, T.L.; Hook, L.A.; Jackson, B.L.

    1993-10-01

    The Background Soil Characterization Project (BSCP) will provide background concentration levels of selected metals, organic compounds, and radionuclides in soils from uncontaminated on-site areas at the Oak Ridge Reservation (ORR), and off-site in the western part of Roane County and the eastern part of Anderson County. The BSCP will establish a database, recommend how to use the data for contaminated site assessment, and provide estimates of the potential human health and environmental risks associated with the background level concentrations of potentially hazardous constituents. ORR background soil characterization data will be used for two purposes. The first application will be in differentiating between naturally occurring constituents and site-related contamination. This is a very important step in a risk assessment because if sufficient background data are not available, no constituent known to be a contaminant can be eliminated from the assessment even if the sampled concentration is measured at a minimum level. The second use of the background data will be in calculating baseline risks against which site-specific contamination risks can be compared

  12. Yucca Mountain Site Characterization Project: Technical Data Catalog (quarterly supplement), June 30, 1994

    1994-01-01

    The DOE/NRC Site-Specific Procedural Agreement for Geologic Repository Site Investigation and Characterization Program requires the DOE to develop and maintain a catalog of data which will be updated and provided to the NRC at least quarterly. This catalog is to include a description of the data; the date, place, and method of acquisition; and where it may be examined. The Yucca Mountain Site Characterization Project (YMP) Technical Data Catalog is published and distributed in accordance with the requirements of the Site-Specific Agreement. The YMP Technical Data Catalog is a report based on reference information contained in the YMP Automated Technical Data Tracking System (ATDT). The reference information is provided by Participants for data acquired or developed in support of the YMP. The Technical Data Catalog is updated quarterly and published in the month following the end of each quarter. A complete revision to the catalog is published at the end of each fiscal year. Supplements to the end-of-year edition are published each quarter. These supplements provide information related to new data items not included in previous quarterly updates and data items affected by changes to previously published reference information. The Technical Data Catalog, dated September 30, 1993, should be retained as the baseline document for the supplements until the end-of-year revision is published and distributed in October 1994

  13. Fiscal Year 1999 memorandum of understanding for the TWRS characterization project

    HUNT, J.W.

    1998-01-01

    During fiscal year 1999, the level of success achieved by the Tank Waste Remediation System (TWRS) shall be determined by specific performance measures. These measures take the form of significant deliverables, one of which is the completion of Tank Characterization Reports (TCRS). In order to achieve success regarding the TCR performance deliverable, multiple organizations across TWRS must work together. Therefore, the requirements and expectations needed from each of these TWRS organizations were examined in order to gain an understanding of the performance necessary from each organization to achieve the end deliverable. This memorandum of understanding (MOU) documents the results of this review and establishes the performance criteria by which TWRS will assess its progress and success. These criteria have been determined based upon a TWRS Characterization Project budget of $42.1 million for fiscal year 1999; if this budget is changed or the currently identified workscope is modified, this MOU will need to be revised accordingly. This MOU is subdivided into six sections, where sections three through six each identify individual interfaces between TWRS organizations. The specific performance criteria related to each TWRS organizational interface are then delineated in the section, along with any additional goals or issues pertaining to that interface

  14. Fiscal year 1998 memorandum of understanding for the TWRS characterization project

    Schreiber, R.D.

    1998-01-01

    During fiscal year 1998, the level of success achieved by the Tank Waste Remediation System (TWRS) shall be determined by specific performance measures. These measures take the form of significant deliverables, one of which is the completion of Tank Characterization Reports (TCRs). In order to achieve success regarding the TCR performance deliverable, multiple organizations across TWRS must work together. Therefore, the requirements and expectations needed from each of these TWRS organizations were examined in order to gain an understanding of the performance necessary from each organization to achieve the end deliverable. This memorandum of understanding (MOU) documents the results of this review and establishes the performance criteria by which TWRS will assess its progress and success. These criteria have been determined based upon a TWRS Characterization Project budget of $47.5 million for fiscal year 1998; if this budget is changed or the currently identified work scope is modified, this MOU will need to be revised accordingly. This MOU is subdivided into six sections, where sections three through six each identify individual interfaces between TWRS organizations. The specific performance criteria related to each TWRS organizational interface are then delineated in the section, along with any additional goals or issues pertaining to that interface

  15. Characterizing uncertain sea-level rise projections to support investment decisions

    Lempert, Robert J.; Wikman-Svahn, Per; Keller, Klaus

    2018-01-01

    Many institutions worldwide are considering how to include uncertainty about future changes in sea-levels and storm surges into their investment decisions regarding large capital infrastructures. Here we examine how to characterize deeply uncertain climate change projections to support such decisions using Robust Decision Making analysis. We address questions regarding how to confront the potential for future changes in low probability but large impact flooding events due to changes in sea-levels and storm surges. Such extreme events can affect investments in infrastructure but have proved difficult to consider in such decisions because of the deep uncertainty surrounding them. This study utilizes Robust Decision Making methods to address two questions applied to investment decisions at the Port of Los Angeles: (1) Under what future conditions would a Port of Los Angeles decision to harden its facilities against extreme flood scenarios at the next upgrade pass a cost-benefit test, and (2) Do sea-level rise projections and other information suggest such conditions are sufficiently likely to justify such an investment? We also compare and contrast the Robust Decision Making methods with a full probabilistic analysis. These two analysis frameworks result in similar investment recommendations for different idealized future sea-level projections, but provide different information to decision makers and envision different types of engagement with stakeholders. In particular, the full probabilistic analysis begins by aggregating the best scientific information into a single set of joint probability distributions, while the Robust Decision Making analysis identifies scenarios where a decision to invest in near-term response to extreme sea-level rise passes a cost-benefit test, and then assembles scientific information of differing levels of confidence to help decision makers judge whether or not these scenarios are sufficiently likely to justify making such investments

  16. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1992 quality program status report

    Bolivar, S.L.; Burningham, A.; Chavez, P.

    1994-03-01

    This status report summarizes the activities and accomplishments of the Los Alamos Yucca Mountain Site Characterization Project's quality assurance program for calendar year 1992. The report includes major sections on Program Activities and Trend Analysis. Program Activities are discussed periodically at quality meetings. The most significant issue addressed in 1992 has been the timely revision of quality administrative procedures. The procedure revision process was streamlined from 55 steps to 7. The number of forms in procedures was reduced by 38%, and the text reduced by 29%. This allowed revision in 1992 of almost half of all implementing procedures. The time necessary to complete the revision process (for a procedure) was reduced from 11 months to 3 months. Other accomplishments include the relaxation of unnecessarily strict training requirements, requiring quality assurance reviews only from affected organizations, and in general simplifying work processes. All members of the YMP received training to the new Orientation class Eleven other training classed were held. Investigators submitted 971 records to the Project and only 37 were rejected. The software program has 115 programs approved for quality-affecting work. The Project Office conducted 3 audits and 1 survey of Los Alamos activities. We conducted 14 audits and 4 surveys. Eight corrective action reports were closed, leaving only one open. Internally, 22 deficiencies were recognized. This is a decrease from 65 in 1991. Since each deficiency requires about 2 man weeks to resolve, the savings are significant. Problems with writing acceptable deficiency reports have essentially disappeared. Trend reports for 1992 were examined and are summarized herein. Three adverse trends have been closed; one remaining adverse trend will be closed when the affected procedures are revised. The number of deficiencies issued to Los Alamos compared to other participants is minimal

  17. Characterizing uncertain sea-level rise projections to support investment decisions.

    Sriver, Ryan L; Lempert, Robert J; Wikman-Svahn, Per; Keller, Klaus

    2018-01-01

    Many institutions worldwide are considering how to include uncertainty about future changes in sea-levels and storm surges into their investment decisions regarding large capital infrastructures. Here we examine how to characterize deeply uncertain climate change projections to support such decisions using Robust Decision Making analysis. We address questions regarding how to confront the potential for future changes in low probability but large impact flooding events due to changes in sea-levels and storm surges. Such extreme events can affect investments in infrastructure but have proved difficult to consider in such decisions because of the deep uncertainty surrounding them. This study utilizes Robust Decision Making methods to address two questions applied to investment decisions at the Port of Los Angeles: (1) Under what future conditions would a Port of Los Angeles decision to harden its facilities against extreme flood scenarios at the next upgrade pass a cost-benefit test, and (2) Do sea-level rise projections and other information suggest such conditions are sufficiently likely to justify such an investment? We also compare and contrast the Robust Decision Making methods with a full probabilistic analysis. These two analysis frameworks result in similar investment recommendations for different idealized future sea-level projections, but provide different information to decision makers and envision different types of engagement with stakeholders. In particular, the full probabilistic analysis begins by aggregating the best scientific information into a single set of joint probability distributions, while the Robust Decision Making analysis identifies scenarios where a decision to invest in near-term response to extreme sea-level rise passes a cost-benefit test, and then assembles scientific information of differing levels of confidence to help decision makers judge whether or not these scenarios are sufficiently likely to justify making such investments

  18. The DLR project Wirbelschleppe. Detecting, characterizing, controlling, attenuating, understanding, and predicting aircraft wake vortices

    Holzaepfel, F. (ed.)

    2008-07-01

    This collection of reports presents an excerpt of the investigations that were performed in the framework of the DLR Projekt Wirbelschleppe. A similar sample of reports was presented as part of three dedicated wake vortex sessions accomplished at the 1{sup st} European Air and Space Conference (CEAS 2007) and Deutscher Luft- und Raumfahrtkongress 2007 in Berlin. The Projekt Wirbelschleppe was conducted in two phases in the time frame from 1999 to 2007 with the five contributing DLR Institutes: Institute of Atmospheric Physics, Institute of Aerodynamics and Flow Technology, Institute of Flight Systems, Institute of Flight Guidance, Institute of Robotics and Mechatronics and the Institute of Aeronautics and Astronautics of the University of Technology Berlin. The project unified a multitude of different aspects and disciplines of wake vortex research which can be characterized by four main themes: - minimization of wake vortices by measures at the aircraft; - development and demonstration of a system for wake vortex prediction and observation; - airborne wake vortex detection and active control; - integration of systems into air traffic control. The Projekt Wirbelschleppe greatly benefited from the European projects AWIATOR, ATC-Wake, Credos, C-Wake, Eurowake, FAR-Wake, FLYSAFE, I-Wake, S-Wake, WakeNet, WakeNet2-Europe, WakeNet3-Europe, and Wavenc. DLR's wake vortex activities will be continued in the Projekt Wetter and Fliegen (2008-2011): Because the current compilation represents only a limited extract of the accomplished work, it is completed by a list of references emerging from the project. (orig.)

  19. Characterizing uncertain sea-level rise projections to support investment decisions.

    Ryan L Sriver

    Full Text Available Many institutions worldwide are considering how to include uncertainty about future changes in sea-levels and storm surges into their investment decisions regarding large capital infrastructures. Here we examine how to characterize deeply uncertain climate change projections to support such decisions using Robust Decision Making analysis. We address questions regarding how to confront the potential for future changes in low probability but large impact flooding events due to changes in sea-levels and storm surges. Such extreme events can affect investments in infrastructure but have proved difficult to consider in such decisions because of the deep uncertainty surrounding them. This study utilizes Robust Decision Making methods to address two questions applied to investment decisions at the Port of Los Angeles: (1 Under what future conditions would a Port of Los Angeles decision to harden its facilities against extreme flood scenarios at the next upgrade pass a cost-benefit test, and (2 Do sea-level rise projections and other information suggest such conditions are sufficiently likely to justify such an investment? We also compare and contrast the Robust Decision Making methods with a full probabilistic analysis. These two analysis frameworks result in similar investment recommendations for different idealized future sea-level projections, but provide different information to decision makers and envision different types of engagement with stakeholders. In particular, the full probabilistic analysis begins by aggregating the best scientific information into a single set of joint probability distributions, while the Robust Decision Making analysis identifies scenarios where a decision to invest in near-term response to extreme sea-level rise passes a cost-benefit test, and then assembles scientific information of differing levels of confidence to help decision makers judge whether or not these scenarios are sufficiently likely to justify making

  20. Environmental assessment for the Groundwater Characterization Project, Nevada Test Site, Nye County, Nevada

    1992-08-01

    The US Department of Energy (DOE) proposes to conduct a program to characterize groundwater at the Nevada Test Site (NTS), Nye County, Nevada, in accordance with a 1987 DOE memorandum stating that all past, present, and future nuclear test sites would be treated as Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) sites (Memorandum from Bruce Green, Weapons Design and Testing Division, June 6, 1987). DOE has prepared an environmental assessment (DOE/EA-0532) to evaluate the environmental consequences associated with the proposed action, referred to as the Groundwater Characterization Project (GCP). This proposed action includes constructing access roads and drill pads, drilling and testing wells, and monitoring these wells for the purpose of characterizing groundwater at the NTS. Long-term monitoring and possible use of these wells in support of CERCLA, as amended by the Superfund Amendments and Reauthorization Act, is also proposed. The GCP includes measures to mitigate potential impacts on sensitive biological, cultural and historical resources, and to protect workers and the environment from exposure to any radioactive or mixed waste materials that may be encountered. DOE considers those mitigation measures related to sensitive biological, cultural and historic resources as essential to render the impacts of the proposed action not significant, and DOE has prepared a Mitigation Action Plan (MAP) that explains how such mitigations will be planned and implemented. Based on the analyses presented in the EA, DOE has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act of 1969 (NEPA). Therefore, preparation of an environmental impact statement is not required and the Department is issuing this FONSI

  1. Environmental assessment for the Groundwater Characterization Project, Nevada Test Site, Nye County, Nevada; Revision 1

    NONE

    1992-08-01

    The US Department of Energy (DOE) proposes to conduct a program to characterize groundwater at the Nevada Test Site (NTS), Nye County, Nevada, in accordance with a 1987 DOE memorandum stating that all past, present, and future nuclear test sites would be treated as Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) sites (Memorandum from Bruce Green, Weapons Design and Testing Division, June 6, 1987). DOE has prepared an environmental assessment (DOE/EA-0532) to evaluate the environmental consequences associated with the proposed action, referred to as the Groundwater Characterization Project (GCP). This proposed action includes constructing access roads and drill pads, drilling and testing wells, and monitoring these wells for the purpose of characterizing groundwater at the NTS. Long-term monitoring and possible use of these wells in support of CERCLA, as amended by the Superfund Amendments and Reauthorization Act, is also proposed. The GCP includes measures to mitigate potential impacts on sensitive biological, cultural and historical resources, and to protect workers and the environment from exposure to any radioactive or mixed waste materials that may be encountered. DOE considers those mitigation measures related to sensitive biological, cultural and historic resources as essential to render the impacts of the proposed action not significant, and DOE has prepared a Mitigation Action Plan (MAP) that explains how such mitigations will be planned and implemented. Based on the analyses presented in the EA, DOE has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act of 1969 (NEPA). Therefore, preparation of an environmental impact statement is not required and the Department is issuing this FONSI.

  2. Characterization of eleven monosomic alien addition lines added from Gossypium anomalum to Gossypium hirsutum using improved GISH and SSR markers.

    Wang, Xiaoxiao; Wang, Yingying; Wang, Chen; Chen, Yu; Chen, Yu; Feng, Shouli; Zhao, Ting; Zhou, Baoliang

    2016-10-07

    Gossypium anomalum (BB genome) possesses the desirable characteristics of drought tolerance, resistance to diseases and insect pests, and the potential for high quality fibers. However, it is difficult to transfer the genes associated with these desirable traits into cultivated cotton (G. hirsutum, AADD genome). Monosomic alien addition lines (MAALs) can be used as a bridge to transfer desired genes from wild species into G. hirsutum. In cotton, however, the high number and smaller size of the chromosomes has resulted in difficulties in discriminating chromosomes from wild species in cultivated cotton background, the development of cotton MAALs has lagged far behind many other crops. To date, no set of G. hirsutum-G. anomalum MAALs was reported. Here the amphiploid (AADDBB genome) derived from G. hirsutum × G. anomalum was used to generate a set of G. hirsutum-G. anomalum MAALs through a combination of consecutive backcrossing, genomic in situ hybridization (GISH), morphological survey and microsatellite marker identification. We improved the GISH technique used in our previous research by using a mixture of two probes from G. anomalum and G. herbaceum (AA genome). The results indicate that a ratio of 4:3 (G. anomalum : G. herbaceum) is the most suitable for discrimination of chromosomes from G. anomalum and the At-subgenome of G. hirsutum. Using this improved GISH technique, 108 MAAL individuals were isolated. Next, 170 G. hirsutum- and G. anomalum-specific codominant markers were obtained and employed for characterization of these MAAL individuals. Finally, eleven out of 13 MAALs were identified. Unfortunately, we were unable to isolate Chrs. 1B a and 5B a due to their very low incidences in backcrossing generation, as these remained in a condition of multiple additions. The characterized lines can be employed as bridges for the transfer of desired genes from G. anomalum into G. hirsutum, as well as for gene assignment, isolation of chromosome

  3. Characterization of a receptor for interleukin-5 on human eosinophils and the myeloid leukemia line HL-60

    Ingley, E.; Young, I.G.

    1991-01-01

    Interleukin-5 (IL-5) promotes the growth and differentiation of human eosinophils and may regulate the selective eosinophilia and eosinophil activation seen in certain diseases. Radiolabeled recombinant human IL-5 (hIL-5) was used to characterize the IL-5 receptor present on normal human eosinophils and on the myeloid leukemia line HL-60, which can be induced to differentiate into eosinophilic cells. Binding studies with eosinophils and HL-60 cells grown under alkaline conditions demonstrated similar high-affinity binding sites for hIL-5 on both cell types with kd values of approximately 400 pmol/L. The binding observed was specific in that it was not inhibited by hIL-3, human granulocyte-macrophage colony-stimulating factor, or hIL-2. Binding studies with a number of other human cell lines, including a B-lymphoma line, and with lymphocyte and neutrophil preparations were also performed, but IL-5 receptors were not detectable on these cells. The number of hIL-5 receptors on HL-60 cells could be correlated with its propensity to differentiate towards an eosinophilic cell type. Expression of hIL-5 receptors on HL-60 cells was upregulated by butyric acid under alkaline conditions, downregulated by hIL-3, virtually eliminated by dimethyl sulfoxide and hIL-5, while hIL-2 had no detectable effect. One major 125I-hIL-5-crosslinked complex of 75 to 85 Kd in Mr was detected on HL-60 cells using crosslinking agents giving a molecular mass of 55 to 60 Kd for the hIL-5 receptor itself. Studies using cellular autoradiography showed that IL-5 receptors were evenly distributed on eosinophils but that receptor distribution on HL-60 cells was noticeably heterogeneous. Eosinophils were the only cells in slides prepared from peripheral blood that had detectable levels of IL-5 receptors in agreement with the specific action of IL-5 on the human eosinophil lineage

  4. Extreme emission-line galaxies out to z ~ 1 in zCOSMOS. I. Sample and characterization of global properties

    Amorín, R.; Pérez-Montero, E.; Contini, T.; Vílchez, J. M.; Bolzonella, M.; Tasca, L. A. M.; Lamareille, F.; Zamorani, G.; Maier, C.; Carollo, C. M.; Kneib, J.-P.; Le Fèvre, O.; Lilly, S.; Mainieri, V.; Renzini, A.; Scodeggio, M.; Bardelli, S.; Bongiorno, A.; Caputi, K.; Cucciati, O.; de la Torre, S.; de Ravel, L.; Franzetti, P.; Garilli, B.; Iovino, A.; Kampczyk, P.; Knobel, C.; Kovač, K.; Le Borgne, J.-F.; Le Brun, V.; Mignoli, M.; Pellò, R.; Peng, Y.; Presotto, V.; Ricciardelli, E.; Silverman, J. D.; Tanaka, M.; Tresse, L.; Vergani, D.; Zucca, E.

    2015-06-01

    Context. The study of large and representative samples of low-metallicity star-forming galaxies at different cosmic epochs is of great interest to the detailed understanding of the assembly history and evolution of low-mass galaxies. Aims: We present a thorough characterization of a large sample of 183 extreme emission-line galaxies (EELGs) at redshift 0.11 ≤ z ≤ 0.93 selected from the 20k zCOSMOS bright survey because of their unusually large emission line equivalent widths. Methods: We use multiwavelength COSMOS photometry, HST-ACS I-band imaging, and optical zCOSMOS spectroscopy to derive the main global properties of star-forming EELGs, such as sizes, stellar masses, star formation rates (SFR), and reliable oxygen abundances using both "direct" and "strong-line" methods. Results: The EELGs are extremely compact (r50 ~ 1.3 kpc), low-mass (M∗ ~ 107-1010 M⊙) galaxies forming stars at unusually high specific star formation rates (sSFR ≡ SFR/M⋆ up to 10-7 yr-1) compared to main sequence star-forming galaxies of the same stellar mass and redshift. At rest-frame UV wavelengths, the EELGs are luminous and show high surface brightness and include strong Lyα emitters, as revealed by GALEX spectroscopy. We show that zCOSMOS EELGs are high-ionization, low-metallicity systems, with median 12+log (O/H) = 8.16 ± 0.21 (0.2 Z⊙) including a handful of extremely metal-deficient (Universe, EELGs are most often found in relative isolation. While only very few EELGs belong to compact groups, almost one third of them are found in spectroscopically confirmed loose pairs or triplets. Conclusions: The zCOSMOS EELGs are galaxies caught in a transient and probably early period of their evolution, where they are efficiently building up a significant fraction of their present-day stellar mass in an ongoing, galaxy-wide starburst. Therefore, the EELGs constitute an ideal benchmark for comparison studies between low- and high-redshift low-mass star-forming galaxies. Full

  5. Methodology for the selection of routes for international cross-border line projects involving multiple objectives and decision-makers in the analyses of restrictions and environmental possibilities

    Angel S, Enrique; Cadena, Luis Fernando

    2005-01-01

    A scheme was developed and applied to select the optimum environmental route for international cross-border line projects, in a decision making context involving multiple objectives and multiple decision-makers, the project studied was the electricity interconnection for central America (SIEPAC) for which a prospective assessment was carried out regarding the restrictions and possibilities in the light of the Colombian environmental dimensions management model. The methodology proposed followed these stages: Definition and approval of the structure of environmental restriction and criticality variables, sectorization and selection of complex sections, definition of decision-makers for multi-objective analysis; design and application of consultation tool; definition and modeling of options applying SIG; sensitivity analysis of alternative routes and project's environment management. Different options were identified for insertion and permanence of the project according to the criteria of various interest groups and actors consulted: environmental authorities, electricity companies, scientific community and civil society

  6. Characterization of Listeria monocytogenes isolated from a fresh mixed sausage processing line in Pelotas-RS by PFGE

    von Laer, Ana Eucares; de Lima, Andréia Saldanha; Trindade, Paula dos Santos; Andriguetto, Cristiano; Destro, Maria Teresa; da Silva, Wladimir Padilha

    2009-01-01

    Listeria monocytogenes is a bacterium capable to adhere to the surfaces of equipment and utensils and subsequently form biofilms. It can to persist in the food processing environmental for extended periods of time being able to contaminate the final product. The aim of this study was to trace the contamination route of L. monocytogenes on a fresh mixed sausage processing line, from raw material to the final product. The isolates obtained were characterized by serotyping and molecular typing by pulsed-field gel electrophoresis (PFGE) using the restriction enzymes ApaI and AscI. L. monocytogenes was detected in 25% of the samples. The samples of raw material were not contaminated, however, the microorganism was detected in 21% of the environmental samples (food contact and non-food contact), 20.8% of the equipments, 20% of the food worker’s hands, 40% of the mass ready to packaging and in all the final products samples, demonstrating that the contamination of final product occurred during the processing and the importance of cross contamination. PFGE yielded 22 pulsotypes wich formed 7 clusters, and serotyping yielded 3 serotypes and 1 serogroup, however, the presence of serotypes 4b and 1/2b in the final product is of great concern for public health. The tracing of contamination showed that some strains are adapted and persisted in the processing environment in this industry. PMID:24031402

  7. Synthesis and Characterization of Silver Nanoparticles Using Cannonball Leaves and Their Cytotoxic Activity against MCF-7 Cell Line

    Devaraj, P.; Kumari, P.; Aarti, Ch.; Renganathan, A.

    2013-01-01

    Cannonball (Couroupita guianensis) is a tree belonging to the family Lecythidaceae. Various parts of the tree have been reported to contain oils, keto steroids, glycosides, couroupitine, indirubin, isatin, and phenolic substances. We report here the synthesis of silver nanoparticles (AgNPs) using cannonball leaves. Green synthesized nanoparticles have been characterized by UV-Vis spectroscopy, SEM, TEM, and FTIR. Cannonball leaf broth as a reducing agent converts silver ions to AgNPs in a rapid and eco friendly manner. The UV-Vis spectra gave surface plasmon resonance peak at 434 nm. TEM image shows well-dispersed silver nanoparticles with an average particle size of 28.4 nm. FTIR showed the structure and respective bands of the synthesized nanoparticles and the stretch of bonds. Green synthesized silver nanoparticles by cannonball leaf extract show cytotoxicity to human breast cancer cell line (MCF-7). Overall, this environmentally friendly method of biological silver nanoparticles production provides rates of synthesis faster than or comparable to those of chemical methods and can potentially be used in various human contacting areas such as cosmetics, foods, and medical applications.

  8. Characterization of inorganic phosphate transport in the triple-negative breast cancer cell line, MDA-MB-231.

    Russo-Abrahão, Thais; Lacerda-Abreu, Marco Antônio; Gomes, Tainá; Cosentino-Gomes, Daniela; Carvalho-de-Araújo, Ayra Diandra; Rodrigues, Mariana Figueiredo; Oliveira, Ana Carolina Leal de; Rumjanek, Franklin David; Monteiro, Robson de Queiroz; Meyer-Fernandes, José Roberto

    2018-01-01

    Recent studies demonstrate that interstitial inorganic phosphate is significantly elevated in the breast cancer microenvironment as compared to normal tissue. In addition it has been shown that breast cancer cells express high levels of the NaPi-IIb carrier (SLC34A2), suggesting that this carrier may play a role in breast cancer progression. However, the biochemical behavior of inorganic phosphate (Pi) transporter in this cancer type remains elusive. In this work, we characterize the kinetic parameters of Pi transport in the aggressive human breast cancer cell line, MDA-MB-231, and correlated Pi transport with cell migration and adhesion. We determined the influence of sodium concentration, pH, metabolic inhibitors, as well as the affinity for inorganic phosphate in Pi transport. We observed that the inorganic phosphate is dependent on sodium transport (K0,5 value = 21.98 mM for NaCl). Furthermore, the transport is modulated by different pH values and increasing concentrations of Pi, following the Michaelis-Menten kinetics (K0,5 = 0.08 mM Pi). PFA, monensin, furosemide and ouabain inhibited Pi transport, cell migration and adhesion. Taken together, these results showed that the uptake of Pi in MDA-MB-231 cells is modulated by sodium and by regulatory mechanisms of intracellular sodium gradient. General Significance: Pi transport might be regarded as a potential target for therapy against tumor progression.

  9. RELIABILITY AND ACCURACY ASSESSMENT OF INVASIVE AND NON- INVASIVE SEISMIC METHODS FOR SITE CHARACTERIZATION: FEEDBACK FROM THE INTERPACIFIC PROJECT

    Garofalo , F.; Foti , S.; Hollender , F.; Bard , P.-Y.; Cornou , C.; Cox , B.R.; Dechamp , A.; Ohrnberger , M.; Sicilia , D.; Vergniault , C.

    2017-01-01

    International audience; The InterPacific project (Intercomparison of methods for site parameter and velocity profile characterization) aims to assess the reliability of seismic site characterization methods (borehole and surface wave methods) used for estimating shear wave velocity (VS) profiles and other related parameters (e.g., VS30). Three sites, representative of different geological conditions relevant for the evaluation of seismic site response effects, have been selected: (1) a hard r...

  10. Standard characterization of soils employed in the FAO/IAEA phosphate project

    Montange, D.; Zapata, F.

    2002-01-01

    In the frame of the FAO/IAEA networked research project, the agronomic effectiveness of natural and modified phosphate rock (PR) products was evaluated using nuclear and related techniques under a variety of soil, climate and management conditions. In addition to the local soil analyses, it was decided to make a standard characterization of the soils employed in the project to gather direct and comparable information on the relevant soil properties affecting the suitability of PRs for direct application and to better interpret the results from the agronomic evaluation, including the creation of a database for phosphate modelling. This paper describes the standard characterization of soils, that was mainly made at CIRAD, Montpellier, France. A total of 51 soil samples were analyzed from 15 countries including Belarus (1), Brazil (2), Chile (3), China (20), Cuba (2) Ghana (6), Hungary (2), Indonesia (3), Kenya (1), Malaysia (1), Poland (1), Romania (2), Russia (1), Thailand (3) and Venezuela (3). Methods of analyses used for the soil characterization included textural class, pH, chemical analysis for total N and P, and exchangeable elements (CEC, saturation). Available P was measured using 4 methods including Olsen, Bray II, Pi paper and Resin. Available P measurements using resin method were made at CENA, Piracicaba, Brazil. The soil P dynamics was described using the 32 P isotope exchange kinetic method at CEN Cadarache, France with the same soil samples. As a result of the worldwide distribution of the soils employed in the project, the results showed a very large diversity in each of the measured soil characteristics. The analysis of the data focused on the most representative tropical acid soils, i.e. Ultisols and Oxisols. Inceptisols have also been included because most of them were acid and located in the tropics and subtropics. Results are synthesized and analyzed with particular emphasis on: i) identification of the most relevant soil characteristics

  11. Neurochemical, morphologic, and laminar characterization of cortical projection neurons in the cingulate motor areas of the macaque monkey

    Nimchinsky, E. A.; Hof, P. R.; Young, W. G.; Morrison, J. H.; Bloom, F. E. (Principal Investigator)

    1996-01-01

    The primate cingulate gyrus contains multiple cortical areas that can be distinguished by several neurochemical features, including the distribution of neurofilament protein-enriched pyramidal neurons. In addition, connectivity and functional properties indicate that there are multiple motor areas in the cortex lining the cingulate sulcus. These motor areas were targeted for analysis of potential interactions among regional specialization, connectivity, and cellular characteristics such as neurochemical profile and morphology. Specifically, intracortical injections of retrogradely transported dyes and intracellular injection were combined with immunocytochemistry to investigate neurons projecting from the cingulate motor areas to the putative forelimb region of the primary motor cortex, area M1. Two separate groups of neurons projecting to area M1 emanated from the cingulate sulcus, one anterior and one posterior, both of which furnished commissural and ipsilateral connections with area M1. The primary difference between the two populations was laminar origin, with the anterior projection originating largely in deep layers, and the posterior projection taking origin equally in superficial and deep layers. With regard to cellular morphology, the anterior projection exhibited more morphologic diversity than the posterior projection. Commissural projections from both anterior and posterior fields originated largely in layer VI. Neurofilament protein distribution was a reliable tool for localizing the two projections and for discriminating between them. Comparable proportions of the two sets of projection neurons contained neurofilament protein, although the density and distribution of the total population of neurofilament protein-enriched neurons was very different in the two subareas of origin. Within a projection, the participating neurons exhibited a high degree of morphologic heterogeneity, and no correlation was observed between somatodendritic morphology and

  12. 46 CFR 160.040-4 - Equipment for impulse-projected rocket type line-throwing appliance.

    2010-10-01

    ... be of either natural or synthetic fibers suitable for marine usage. The end of the line intended to... directions for loading and firing the appliance in service use shall be permanently engraved in plastic and...

  13. Methodology and main results of seismic source characterization for the PEGASOS Project, Switzerland

    Coppersmith, K. J.; Youngs, R. R.; Sprecher, Ch.

    2009-01-01

    Under the direction of the National Cooperative for the Disposal of Radioactive Waste (NAGRA), a probabilistic seismic hazard analysis was conducted for the Swiss nuclear power plant sites. The study has become known under the name 'PEGASOS Project'. This is the first of a group of papers in this volume that describes the seismic source characterization methodology and the main results of the project. A formal expert elicitation process was used, including dissemination of a comprehensive database, multiple workshops for identification and discussion of alternative models and interpretations, elicitation interviews, feedback to provide the experts with the implications of their preliminary assessments, and full documentation of the assessments. A number of innovative approaches to the seismic source characterization methodology were developed by four expert groups and implemented in the study. The identification of epistemic uncertainties and treatment using logic trees were important elements of the assessments. Relative to the assessment of the seismotectonic framework, the four expert teams identified similar main seismotectonic elements: the Rhine Graben, the Jura / Molasse regions, Helvetic and crystalline subdivisions of the Alps, and the southern Germany region. In defining seismic sources, the expert teams used a variety of approaches. These range from large regional source zones having spatially-smoothed seismicity to smaller local zones, to account for spatial variations in observed seismicity. All of the teams discussed the issue of identification of feature-specific seismic sources (i.e. individual mapped faults) as well as the potential reactivation of the boundary faults of the Permo-Carboniferous grabens. Other important seismic source definition elements are the specification of earthquake rupture dimensions and the earthquake depth distribution. Maximum earthquake magnitudes were assessed for each seismic source using approaches that consider the

  14. Sierra Pacific Power Company Alturas Transmission Line Project, Final Environmental Impact Report/Environmental Impact Statement. Volume 2: Comments and responses

    1995-11-01

    Sierra Pacific Power Company has proposed the construction and operation of a 345,000 volt overhead electric power transmission line from Alturas, California to Reno, Nevada. This Environmental Impact Report/Environmental Impact Statement will assess the potential environmental impacts of the proposed project and alternatives. This report contains public comments which were received on the Draft EIR/S. Significant issues may be identified through public and agency comments

  15. Status of volcanism studies for the Yucca Mountain Site Characterization Project

    Crowe, B.; Perry, F.; Murrell, M.; Poths, J.; Valentine, G.A. [Los Alamos National Lab., NM (United States); Wells, S. [Univ. of California, Riverside, CA (United States); Bowker, L.; Finnegan, K. [Univ. of Nevada, Las Vegas, NV (United States); Geissman, J.; McFadden, L.

    1995-02-01

    Chapter 1 introduces the volcanism issue for the Yucca Mountain site and provides the reader with an overview of the organization, content, and significant conclusions of this report. The risk of future basaltic volcanism is the primary topic of concern including both events that intersect a potential repository and events that occur near or within the waste isolation system of a repository. Chapter 2 describes the volcanic history of the Yucca Mountain region (YMR) and emphasizes the Pliocene and Quaternary volcanic record, the interval of primary concern for volcanic risk assessment. The Lathrop Wells volcanic center is described in detail because it is the youngest basalt center in the YMR. Chapter 3 describes the tectonic setting of the YMR and presents and assesses the significance of multiple alternative tectonic models. Geophysical data are described for the YMR and are used as an aid to understand the distribution of basaltic volcanic centers. Chapter 4 discusses the petrologic and geochemical features of basaltic volcanism in the YMR, the southern Great Basin and the Basin and Range province. The long time of activity and characteristic small volume of the Postcaldera basalt of the YMR result in one of the lowest eruptive rates in a volcanic field in the southwest United States. Chapter 5 summarizes current concepts of the segregation, ascent, and eruption of basalt magma. Chapter 6 summarizes the history of volcanism studies (1979 through early 1994), including work for the Yucca Mountain Site Characterization Project and overview studies by the state of Nevada and the Nuclear Regulatory Commission. Chapter 7 summarizes probabilistic volcanic hazard assessment using a three-part conditional probability model. Chapter 8 describes remaining volcanism work judged to be needed to complete characterization studies for the YMR. Chapter 9 summarizes the conclusions of this volcanism status report.

  16. Status of volcanism studies for the Yucca Mountain Site Characterization Project

    Crowe, B.; Perry, F.; Murrell, M.; Poths, J.; Valentine, G.A.; Wells, S.; Bowker, L.; Finnegan, K.; Geissman, J.; McFadden, L.

    1995-02-01

    Chapter 1 introduces the volcanism issue for the Yucca Mountain site and provides the reader with an overview of the organization, content, and significant conclusions of this report. The risk of future basaltic volcanism is the primary topic of concern including both events that intersect a potential repository and events that occur near or within the waste isolation system of a repository. Chapter 2 describes the volcanic history of the Yucca Mountain region (YMR) and emphasizes the Pliocene and Quaternary volcanic record, the interval of primary concern for volcanic risk assessment. The Lathrop Wells volcanic center is described in detail because it is the youngest basalt center in the YMR. Chapter 3 describes the tectonic setting of the YMR and presents and assesses the significance of multiple alternative tectonic models. Geophysical data are described for the YMR and are used as an aid to understand the distribution of basaltic volcanic centers. Chapter 4 discusses the petrologic and geochemical features of basaltic volcanism in the YMR, the southern Great Basin and the Basin and Range province. The long time of activity and characteristic small volume of the Postcaldera basalt of the YMR result in one of the lowest eruptive rates in a volcanic field in the southwest United States. Chapter 5 summarizes current concepts of the segregation, ascent, and eruption of basalt magma. Chapter 6 summarizes the history of volcanism studies (1979 through early 1994), including work for the Yucca Mountain Site Characterization Project and overview studies by the state of Nevada and the Nuclear Regulatory Commission. Chapter 7 summarizes probabilistic volcanic hazard assessment using a three-part conditional probability model. Chapter 8 describes remaining volcanism work judged to be needed to complete characterization studies for the YMR. Chapter 9 summarizes the conclusions of this volcanism status report

  17. Response to state comments on the revised draft North Central Regional characterization reports for the Crystalline Repository Project

    1985-08-01

    The purpose of this document is to respond to the comments received from the states of the North Central Region on the revised draft North Central Regional Characterization Reports (RCRs). The responses in this document indicate the manner in which the suggestions or comments received have been considered in modifying the revised draft North Central RCRs. Both general comments related to the overall Crystalline Repository Project (CRP) and comments on specific sections of the RCRs are addressed. This document responds to North Central State comments on both the revised draft North Central Regional Geologic Characterization Report (RGCR) and the revised draft North Central Regional Environmental Characterization Report (RECR)

  18. Response to state comments on the revised draft Southeastern Regional Characterization Reports for the Crystalline Repository Project

    1985-08-01

    The purpose of this document is to respond to the comments received from the states of the Southeastern Region on the revised draft Southeastern Regional Characterization Reports (RCRs). The responses in this document indicate the manner in which the suggestions or comments received have been considered in modifying the revised draft Southeastern RCRs. Both general comments related to the overall Crystalline Repository Project (CRP) and comments on specific sections of the RCRs are addressed. This document responds to Southeastern State comments on both the revised draft Southeastern Regional Geologic Characterization Report (RGCR) and the revised draft Southeastern Regional Environmental Characterization Report (RECR)

  19. Response to state comments on the revised draft northeastern regional characterization reports for the Crystalline Repository Project

    1985-08-01

    The purpose of this document is to respond to the comments received from the States of the Northeastern Region on the revised draft Northeastern Regional Characterization Reports (RCRs). The responses in this document indicate the manner in which the suggestions or comments received have been considered in modifying the revised draft Northeastern RCRs. Both general comments related to the overall Crystalline Repository Project (CRP) and comments on specific sections of the RCRs are addressed. This document responds to Northeastern State comments on both the revised draft Northeastern Regional Geologic Characterization Report (RGCR) and the revised draft Northeastern Regional Environmental Characterization Report (RECR)

  20. Estimates of spatial correlation in volcanic tuff, Yucca Mountain, Nevada: Yucca Mountain Site Characterization Project

    Rautman, C.A.

    1991-02-01

    The spatial correlation structure of volcanic tuffs at and near the site of the proposed high-level nuclear waste repository at Yucca Mountain, Nevada, is estimated using samples obtained from surface outcrops and drill holes. Data are examined for four rock properties: porosity, air permeability, saturated hydraulic conductivity, and dry bulk density. Spatial continuity patterns are identified in both lateral and vertical (stratigraphic) dimensions. The data are examined for the Calico Hills tuff stratigraphic unit and also without regard for stratigraphy. Variogram models fitted to the sample data from the tuffs of Calico Hills indicate that porosity is correlated laterally over distances of up to 3000 feet. If air permeability and saturated conductivity values are viewed as semi-interchangeable for purposes of identifying spatial structure, the data suggest a maximum range of correlation of 300 to 500 feet without any obvious horizontal to vertical anisotropy. Continuity exists over vertical distances of roughly 200 feet. Similar variogram models fitted to sample data taken from vertical drill holes without regard for stratigraphy suggest that correlation exists over distances of 500 to 800 feet for each rock property examined. Spatial correlation of rock properties violates the sample-independence assumptions of classical statistics to a degree not usually acknowledged. In effect, the existence of spatial structure reduces the ''equivalent'' number of samples below the number of physical samples. This reduction in the effective sampling density has important implications for site characterization for the Yucca Mountain Project. 19 refs., 43 figs., 5 tabs

  1. Hydrometric, Hydrochemical, and Hydrogeophysical Runoff Characterization Across Multiple Land Covers in the Agua Salud Project, Panama

    Litt, Guy Finley

    As the Panama Canal Authority faces sensitivity to water shortages, managing water resources becomes crucial for the global shipping industry's security. These studies address knowledge gaps in tropical water resources to aid hydrological model development and validation. Field-based hydrological investigations in the Agua Salud Project within the Panama Canal Watershed employed multiple tools across a variety of land covers to investigate hydrological processes. Geochemical tracers informed where storm runoff in a stream comes from and identified electrical conductivity (EC) as an economical, high sample frequency tracer during small storms. EC-based hydrograph separation coupled with hydrograph recession rate analyses identified shallow and deep groundwater storage-discharge relationships that varied by season and land cover. A series of plot-scale electrical resistivity imaging geophysical experiments coupled with rainfall simulation characterized subsurface flow pathway behavior and quantified respectively increasing infiltration rates across pasture, 10 year old secondary succession forest, teak (tectona grandis), and 30 year old secondary succession forest land covers. Additional soil water, groundwater, and geochemical studies informed conceptual model development in subsurface flow pathways and groundwater, and identified future research needs.

  2. Characterization of projected DWPF glasses heat treated to simulate canister centerline cooling

    Marra, S.L.; Jantzen, C.M.

    1992-05-01

    Liquid high-level nuclear waste will be immobilized at the Savannah River Site (SRS) by vitrification in borosilicate glass. The glass will be produced and poured into stainless steel canisters in the Defense Waste Processing Facility (DWPF). Eventually these canistered waste forms will be sent to a geologic repository for final disposal. In order to assure acceptability by the repository, the Department of Energy has defined requirements which DWPF canistered waste forms must meet. These requirements are the Waste Acceptance Preliminary Specifications (WAPS). The WAPS require DWPF to identify the crystalline phases expected to be present in the final glass product. Knowledge of the thermal history of the borosilicate glass during filling and cooldown of the canister is necessary to determine the amount and type of crystalline phases present in the final glass product. Glass samples of seven projected DWPF compositions were cooled following the same temperature profile as that of glass at the centerline of the full-scale DWPF canister. The glasses were characterized by x-ray diffraction and scanning electron microscopy to identify the crystalline phases present The volume percents of each crystalline phase present were determined by quantitative x-ray diffraction. The Product Consistency Test (PCI) was used to determine the durability of the heat-treated glasses

  3. Evaluation of low-level radioactive waste characterization and classification programs of the West Valley Demonstration Project

    Taie, K.R.

    1994-01-01

    The West Valley Demonstration Project (WVDP) is preparing to upgrade their low-level radioactive waste (LLW) characterization and classification program. This thesis describes a survey study of three other DOE sites conducted in support of this effort. The LLW characterization/classification programs of Oak Ridge National Laboratory, Savannah River Site, and Idaho National Engineering Laboratory were critically evaluated. The evaluation was accomplished through tours of each site facility and personnel interviews. Comparative evaluation of the individual characterization/classification programs suggests the WVDP should purchase a real-time radiography unit and a passive/active neutron detection system, make additional mechanical modifications to the segmented gamma spectroscopy assay system, provide a separate building to house characterization equipment and perform assays away from waste storage, develop and document a new LLW characterization/classification methodology, and make use of the supercompactor owned by WVDP

  4. Characterization of materials for a reactive transport model validation experiment: Interim report on the caisson experiment. Yucca Mountain Site Characterization Project

    Siegel, M.D.; Cheng, W.C.; Ward, D.B.; Bryan, C.R.

    1995-08-01

    Models used in performance assessment and site characterization activities related to nuclear waste disposal rely on simplified representations of solute/rock interactions, hydrologic flow field and the material properties of the rock layers surrounding the repository. A crucial element in the design of these models is the validity of these simplifying assumptions. An intermediate-scale experiment is being carried out at the Experimental Engineered Test Facility at Los Alamos Laboratory by the Los Alamos and Sandia National Laboratories to develop a strategy to validate key geochemical and hydrological assumptions in performance assessment models used by the Yucca Mountain Site Characterization Project

  5. Characterization of materials for a reactive transport model validation experiment: Interim report on the caisson experiment. Yucca Mountain Site Characterization Project

    Siegel, M.D.; Cheng, W.C. [Sandia National Labs., Albuquerque, NM (United States); Ward, D.B.; Bryan, C.R. [Univ. of New Mexico, Albuquerque, NM (United States). Dept. of Earth and Planetary Sciences

    1995-08-01

    Models used in performance assessment and site characterization activities related to nuclear waste disposal rely on simplified representations of solute/rock interactions, hydrologic flow field and the material properties of the rock layers surrounding the repository. A crucial element in the design of these models is the validity of these simplifying assumptions. An intermediate-scale experiment is being carried out at the Experimental Engineered Test Facility at Los Alamos Laboratory by the Los Alamos and Sandia National Laboratories to develop a strategy to validate key geochemical and hydrological assumptions in performance assessment models used by the Yucca Mountain Site Characterization Project.

  6. Synthesis and Characterization of New Palladium(II) Thiosemicarbazone Complexes and Their Cytotoxic Activity against Various Human Tumor Cell Lines

    Hernández, Wilfredo; Paz, Juan; Carrasco, Fernando; Spodine, Evgenia; Manzur, Jorge; Sieler, Joachim; Blaurock, Steffen; Beyer, Lothar

    2013-01-01

    The palladium(II) bis-chelate complexes of the type [Pd(TSC1-5)2] (6–10), with their corresponding ligands 4-phenyl-1-(acetone)-thiosemicarbazone, HTSC1 (1), 4-phenyl-1-(2′-chloro-benzaldehyde)-thiosemicarbazone, HTSC2 (2), 4-phenyl-1-(3′-hydroxy-benzaldehyde)-thiosemicarbazone, HTSC3 (3), 4-phenyl-1-(2′-naphthaldehyde)-thiosemicarbazone, HTSC4 (4), and 4-phenyl-1-(1′-nitro-2′-naphthaldehyde)-thiosemicarbazone, HTSC5 (5), were synthesized and characterized by elemental analysis and spectroscopic techniques (IR and 1H- and 13C-NMR). The molecular structure of HTSC3, HTSC4, and [Pd(TSC1)2] (6) have been determined by single crystal X-ray crystallography. Complex 6 shows a square planar geometry with two deprotonated ligands coordinated to PdII through the azomethine nitrogen and thione sulfur atoms in a cis arrangement. The in vitro cytotoxic activity measurements indicate that the palladium(II) complexes (IC50 = 0.01–9.87 μM) exhibited higher antiproliferative activity than their free ligands (IC50 = 23.48–70.86 and >250 μM) against different types of human tumor cell lines. Among all the studied palladium(II) complexes, the [Pd(TSC3)2] (8) complex exhibited high antitumor activity on the DU145 prostate carcinoma and K562 chronic myelogenous leukemia cells, with low values of the inhibitory concentration (0.01 and 0.02 μM, resp.). PMID:24391528

  7. Characterization and classification of one new cytoplasmic male sterility (CMS) line based on morphological, cytological and molecular markers in non-heading Chinese cabbage (Brassica rapa L.).

    Heng, Shuangping; Shi, Dianyi; Hu, Zhenhua; Huang, Tao; Li, Jinping; Liu, Liyan; Xia, Chunxiu; Yuan, Zhenzhen; Xu, Yuejin; Fu, Tingdong; Wan, Zhengjie

    2015-09-01

    A new non-heading Chinese cabbage CMS line M119A was characterized and specific molecular markers were developed to classify different CMS types. One new non-heading Chinese cabbage (Brassica rapa L.) cytoplasmic male sterile (CMS) line M119A was obtained by interspecific crosses between the recently discovered hau CMS line of Brassica juncea and B. rapa. Furthermore, the line was characterized and compared with other five isonuclear-alloplasmic CMS lines. The M119A line produced six stamens without pollen and only two stamen fused together in fewer flowers. Tissue section indicated that anther abortion in M119A may have occurred during differentiation of the archesporial cells without pollen sac. All the six CMS lines were grouped into three types based on the presence of three PCR fragments of 825, 465 and 772 bp amplified with different mitochondrial genes specific primers. The 825-bp fragment was amplified both in 09-10A and H201A using the specific primer pair P-orf224-atp6, and showed 100 % identity with the mitochondrial gene of pol CMS. The 465-bp fragment was amplified in 30A and 105A using the primer pair P-orf138 and shared 100 % identity with the mitochondrial gene of ogu CMS. The 772-bp fragment was amplified in M119A and H203A using the primer pair P-orf288 and showed 100 % identity with the mitochondrial gene of hau CMS. Therefore, these markers could efficiently distinguish different types of isonuclear-alloplasmic CMS lines of non-heading Chinese cabbage, which were useful for improving the efficiency of cross-breeding and heterosis utilization in cruciferous vegetables.

  8. On-line Flagging of Anomalies and Adaptive Sequential Hypothesis Testing for Fine-feature Characterization of Geosynchronous Satellites

    Chaudhary, A.; Payne, T.; Kinateder, K.; Dao, P.; Beecher, E.; Boone, D.; Elliott, B.

    The objective of on-line flagging in this paper is to perform interactive assessment of geosynchronous satellites anomalies such as cross-tagging of a satellites in a cluster, solar panel offset change, etc. This assessment will utilize a Bayesian belief propagation procedure and will include automated update of baseline signature data for the satellite, while accounting for the seasonal changes. Its purpose is to enable an ongoing, automated assessment of satellite behavior through its life cycle using the photometry data collected during the synoptic search performed by a ground or space-based sensor as a part of its metrics mission. The change in the satellite features will be reported along with the probabilities of Type I and Type II errors. The objective of adaptive sequential hypothesis testing in this paper is to define future sensor tasking for the purpose of characterization of fine features of the satellite. The tasking will be designed in order to maximize new information with the least number of photometry data points to be collected during the synoptic search by a ground or space-based sensor. Its calculation is based on the utilization of information entropy techniques. The tasking is defined by considering a sequence of hypotheses in regard to the fine features of the satellite. The optimal observation conditions are then ordered in order to maximize new information about a chosen fine feature. The combined objective of on-line flagging and adaptive sequential hypothesis testing is to progressively discover new information about the features of a geosynchronous satellites by leveraging the regular but sparse cadence of data collection during the synoptic search performed by a ground or space-based sensor. Automated Algorithm to Detect Changes in Geostationary Satellite's Configuration and Cross-Tagging Phan Dao, Air Force Research Laboratory/RVB By characterizing geostationary satellites based on photometry and color photometry, analysts can

  9. The SAURON project - XI. Stellar populations from absorption-line strength maps of 24 early-type spirals

    Peletier, Reynier F.; Falcon-Barroso, Jesus; Bacon, Roland; Cappellari, Michele; Davies, Roger L.; de Zeeuw, P. T.; Emsellem, Eric; Ganda, Katia; Krajnovic, Davor; Kuntschner, Harald; McDermid, Richard M.; Sarzi, Marc; van de Ven, Glenn

    2007-01-01

    We present absorption-line strength maps of a sample of 24 representative early-type spiral galaxies, mostly of type Sa, obtained as part of the SAURON (Spectrographic Areal Unit for Research on Optical Nebulae) survey of nearby galaxies using our custom-built integral-field spectrograph. Using

  10. 75 FR 75170 - Minnkota Power Cooperative, Inc.: Bemidji to Grand Rapids 230 kV Transmission Line Project

    2010-12-02

    ... availability of Record of Decision. SUMMARY: The Rural Utilities Service, hereinafter referred to as RUS and/or the Agency, has issued a Record of Decision (ROD) for the Environmental Impact Statement (EIS) for the... financing to construct the 230 kilovolt (kV) transmission line between the Wilton Substation near Bemidji...

  11. The LCO Follow-up and Characterization Network and AgentNEO Citizen Science Project

    Lister, Tim; Greenstreet, Sarah; Gomez, Edward; Christensen, Eric J.; Larson, Stephen M.

    2017-10-01

    The LCO NEO Follow-up Network is using the telescopes of the Las Cumbres Observatory (LCO) and a web-based target selection, scheduling and data reduction system to confirm NEO candidates and characterize radar-targeted known NEOs. Starting in July 2014, the LCO NEO Follow-up Network has observed over 4,500 targets and reported more than 25,000 astrometric and photometric measurements to the Minor Planet Center.The LCO NEO Follow-up Network's main aims are to perform confirming follow-up of the large number of NEO candidates and to perform characterization measurements of radar targets to obtain light curves and rotation rates. The NEO candidates come from the NEO surveys such as Catalina, PanSTARRS, ATLAS, NEOWISE and others. In particular, we are targeting objects in the Southern Hemisphere, where the LCO NEO Follow-up Network is the largest resource for NEO observations.The first phase of the LCO Network comprises nine 1-meter and seven 0.4-meter telescopes at site at McDonald Observatory (Texas), Cerro Tololo (Chile), SAAO (South Africa) and Siding Spring Observatory (Australia). The network has been fully operational since 2014 May, and observations are being executed remotely and robotically. Additional 0.4-meter telescopes will be deployed in 2017 and 2x1-meter telescopes for a site at Ali Observatory, Tibet are planned for 2018-2019.We have developed web-based software called NEOexchange which automatically downloads and aggregates NEO candidates from the Minor Planet Center's NEO Confirmation Page, the Arecibo and Goldstone radar target lists and the NASA lists. NEOexchange allows the planning and scheduling of observations on the LCO Telescope Network and the tracking of the resulting blocks and generated data. We have extended the NEOexchange software to include automated scheduling and moving object detection, with the results presented to the user via the website.We will present results from the LCO NEO Follow-up Network and from the development of the

  12. On-Line Use of Three-Dimensional Marker Trajectory Estimation From Cone-Beam Computed Tomography Projections for Precise Setup in Radiotherapy for Targets With Respiratory Motion

    Worm, Esben S.; Høyer, Morten; Fledelius, Walther; Nielsen, Jens E.; Larsen, Lars P.; Poulsen, Per R.

    2012-01-01

    Purpose: To develop and evaluate accurate and objective on-line patient setup based on a novel semiautomatic technique in which three-dimensional marker trajectories were estimated from two-dimensional cone-beam computed tomography (CBCT) projections. Methods and Materials: Seven treatment courses of stereotactic body radiotherapy for liver tumors were delivered in 21 fractions in total to 6 patients by a linear accelerator. Each patient had two to three gold markers implanted close to the tumors. Before treatment, a CBCT scan with approximately 675 two-dimensional projections was acquired during a full gantry rotation. The marker positions were segmented in each projection. From this, the three-dimensional marker trajectories were estimated using a probability based method. The required couch shifts for patient setup were calculated from the mean marker positions along the trajectories. A motion phantom moving with known tumor trajectories was used to examine the accuracy of the method. Trajectory-based setup was retrospectively used off-line for the first five treatment courses (15 fractions) and on-line for the last two treatment courses (6 fractions). Automatic marker segmentation was compared with manual segmentation. The trajectory-based setup was compared with setup based on conventional CBCT guidance on the markers (first 15 fractions). Results: Phantom measurements showed that trajectory-based estimation of the mean marker position was accurate within 0.3 mm. The on-line trajectory-based patient setup was performed within approximately 5 minutes. The automatic marker segmentation agreed with manual segmentation within 0.36 ± 0.50 pixels (mean ± SD; pixel size, 0.26 mm in isocenter). The accuracy of conventional volumetric CBCT guidance was compromised by motion smearing (≤21 mm) that induced an absolute three-dimensional setup error of 1.6 ± 0.9 mm (maximum, 3.2) relative to trajectory-based setup. Conclusions: The first on-line clinical use of

  13. A characterization of the ZFL cell line and primary hepatocytes as in vitro liver cell models for the zebrafish (Danio rerio)

    Eide, Marta; Rusten, Marte; Male, Rune; Jensen, Knut Helge Midtbø; Goksøyr, Anders

    2014-01-01

    Highlights: •The ZFL cell line and primary hepatocytes were characterized. •Basic and induced expression of nuclear receptors and target genes were found. •The ZFL cell line expresses very low basic levels of most genes. •The ZFL cells have low induction of gene expression following exposures. •Primary hepatocytes show large sex-dependent differences in gene expression. -- Abstract: The zebrafish (Danio rerio) is a widely used model species in biomedical research. The ZFL cell line, established from zebrafish liver, and freshly isolated primary hepatocytes from zebrafish have been used in several toxicological studies. However, no previous report has compared and characterized these two systems at the level of gene expression. The aim of this study was to evaluate the ZFL cell line in comparison to primary hepatocytes as in vitro models for studying effects of environmental contaminants in zebrafish liver. Using quantitative real-time PCR, the basal level and transcriptional induction potential of key genes involved in toxic responses in the ZFL cell line, primary hepatocytes and whole liver from zebrafish were compared. The study showed that the ZFL cells have lower levels of mRNA of most selected genes compared to zebrafish liver. The induced gene transcription following exposure to ligand was much lower in ZFL cells compared to zebrafish primary hepatocytes at the doses tested. Importantly, oestrogen receptor and vitellogenin genes showed low basal transcription and no induction response in the ZFL cell line. In conclusion, it appears that primary hepatocytes are well suited for studying environmental contaminants including xenoestrogens, but may show large sex-dependent differences in gene transcription. The ZFL cell line shows potential in toxicological studies involving the aryl hydrocarbon receptor pathway. However, low potential for transcriptional induction of genes in general should be expected, especially notable when studying estrogenic

  14. A characterization of the ZFL cell line and primary hepatocytes as in vitro liver cell models for the zebrafish (Danio rerio)

    Eide, Marta, E-mail: marta.eide@bio.uib.no [Department of Biology, University of Bergen, Bergen (Norway); Rusten, Marte; Male, Rune [Department of Molecular Biology, University of Bergen, Bergen (Norway); Jensen, Knut Helge Midtbø; Goksøyr, Anders [Department of Biology, University of Bergen, Bergen (Norway)

    2014-02-15

    Highlights: •The ZFL cell line and primary hepatocytes were characterized. •Basic and induced expression of nuclear receptors and target genes were found. •The ZFL cell line expresses very low basic levels of most genes. •The ZFL cells have low induction of gene expression following exposures. •Primary hepatocytes show large sex-dependent differences in gene expression. -- Abstract: The zebrafish (Danio rerio) is a widely used model species in biomedical research. The ZFL cell line, established from zebrafish liver, and freshly isolated primary hepatocytes from zebrafish have been used in several toxicological studies. However, no previous report has compared and characterized these two systems at the level of gene expression. The aim of this study was to evaluate the ZFL cell line in comparison to primary hepatocytes as in vitro models for studying effects of environmental contaminants in zebrafish liver. Using quantitative real-time PCR, the basal level and transcriptional induction potential of key genes involved in toxic responses in the ZFL cell line, primary hepatocytes and whole liver from zebrafish were compared. The study showed that the ZFL cells have lower levels of mRNA of most selected genes compared to zebrafish liver. The induced gene transcription following exposure to ligand was much lower in ZFL cells compared to zebrafish primary hepatocytes at the doses tested. Importantly, oestrogen receptor and vitellogenin genes showed low basal transcription and no induction response in the ZFL cell line. In conclusion, it appears that primary hepatocytes are well suited for studying environmental contaminants including xenoestrogens, but may show large sex-dependent differences in gene transcription. The ZFL cell line shows potential in toxicological studies involving the aryl hydrocarbon receptor pathway. However, low potential for transcriptional induction of genes in general should be expected, especially notable when studying estrogenic

  15. Conventional and molecular cytogenetics of human non-medullary thyroid carcinoma: characterization of eight cell line models and review of the literature on clinical samples

    Ribeiro, Franclim Ricardo; Meireles, Ana Margarida; Rocha, Ana Sofia; Teixeira, Manuel Rodrigues

    2008-01-01

    Cell lines are often poorly characterized from a genetic point of view, reducing their usefulness as tumor models. Our purpose was to assess the genetic background of eight commonly used human thyroid carcinoma models and to compare the findings with those reported for primary tumors of the gland. We used chromosome banding analysis and comparative genomic hybridization to profile eight non-medullary thyroid carcinoma cell lines of papillary (TPC-1, FB2, K1 and B-CPAP), follicular (XTC-1) or anaplastic origin (8505C, C643 and HTH74). To assess the representativeness of the findings, we additionally performed a thorough review of cytogenetic (n = 125) and DNA copy number information (n = 270) available in the literature on clinical samples of thyroid carcinoma. The detailed characterization of chromosomal markers specific for each cell line revealed two cases of mistaken identities: FB2 was shown to derive from TPC-1 cells, whereas K1 cells have their origin in cell line GLAG-66. All cellular models displayed genomic aberrations of varying complexity, and recurrent gains at 5p, 5q, 8q, and 20q (6/7 cell lines) and losses at 8p, 13q, 18q, and Xp (4/7 cell lines) were seen. Importantly, the genomic profiles were compatible with those of the respective primary tumors, as seen in the meta-analysis of the existing literature data. We provide the genomic background of seven independent thyroid carcinoma models representative of the clinical tumors of the corresponding histotypes, and highlight regions of recurrent aberrations that may guide future studies aimed at identifying target genes. Our findings further support the importance of routinely performing cytogenetic studies on cell lines, to detect cross-contamination mishaps such as those identified here

  16. Phase 1 Characterization sampling and analysis plan West Valley demonstration project.

    Johnson, R. L. (Environmental Science Division)

    2011-06-30

    The Phase 1 Characterization Sampling and Analysis Plan (CSAP) provides details about environmental data collection that will be taking place to support Phase 1 decommissioning activities described in the Phase 1 Decommissioning Plan for the West Valley Demonstration Project, Revision 2 (Phase I DP; DOE 2009). The four primary purposes of CSAP data collection are: (1) pre-design data collection, (2) remedial support, (3) post-remediation status documentation, and (4) Phase 2 decision-making support. Data collection to support these four main objectives is organized into two distinct data collection efforts. The first is data collection that will take place prior to the initiation of significant Phase 1 decommissioning activities (e.g., the Waste Management Area [WMA] 1 and WMA 2 excavations). The second is data collection that will occur during and immediately after environmental remediation in support of remediation activities. Both data collection efforts have a set of well-defined objectives that encompass the data needs of the four main CSAP data collection purposes detailed in the CSAP. The main body of the CSAP describes the overall data collection strategies that will be used to satisfy data collection objectives. The details of pre-remediation data collection are organized by WMA. The CSAP contains an appendix for each WMA that describes the details of WMA-specific pre-remediation data collection activities. The CSAP is intended to expand upon the data collection requirements identified in the Phase 1 Decommissioning Plan. The CSAP is intended to tightly integrate with the Phase 1 Final Status Survey Plan (FSSP). Data collection described by the CSAP is consistent with the FSSP where appropriate and to the extent possible.

  17. Sequence Analysis and Characterization of Active Human Alu Subfamilies Based on the 1000 Genomes Pilot Project.

    Konkel, Miriam K; Walker, Jerilyn A; Hotard, Ashley B; Ranck, Megan C; Fontenot, Catherine C; Storer, Jessica; Stewart, Chip; Marth, Gabor T; Batzer, Mark A

    2015-08-29

    The goal of the 1000 Genomes Consortium is to characterize human genome structural variation (SV), including forms of copy number variations such as deletions, duplications, and insertions. Mobile element insertions, particularly Alu elements, are major contributors to genomic SV among humans. During the pilot phase of the project we experimentally validated 645 (611 intergenic and 34 exon targeted) polymorphic "young" Alu insertion events, absent from the human reference genome. Here, we report high resolution sequencing of 343 (322 unique) recent Alu insertion events, along with their respective target site duplications, precise genomic breakpoint coordinates, subfamily assignment, percent divergence, and estimated A-rich tail lengths. All the sequenced Alu loci were derived from the AluY lineage with no evidence of retrotransposition activity involving older Alu families (e.g., AluJ and AluS). AluYa5 is currently the most active Alu subfamily in the human lineage, followed by AluYb8, and many others including three newly identified subfamilies we have termed AluYb7a3, AluYb8b1, and AluYa4a1. This report provides the structural details of 322 unique Alu variants from individual human genomes collectively adding about 100 kb of genomic variation. Many Alu subfamilies are currently active in human populations, including a surprising level of AluY retrotransposition. Human Alu subfamilies exhibit continuous evolution with potential drivers sprouting new Alu lineages. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  18. A strategy to seal exploratory boreholes in unsaturated tuff; Yucca Mountain Site Characterization Project

    Fernandez, J.A. [Sandia National Labs., Albuquerque, NM (United States); Case, J.B.; Givens, C.A.; Carney, B.C. [IT Corp., Albuquerque, NM (United States)

    1994-04-01

    This report presents a strategy for sealing exploratory boreholes associated with the Yucca Mountain Site Characterization Project. Over 500 existing and proposed boreholes have been considered in the development of this strategy, ranging from shallow (penetrating into alluvium only) to deep (penetrating into the groundwater table). Among the comprehensive list of recommendations are the following: Those boreholes within the potential repository boundary and penetrating through the potential repository horizon are the most significant boreholes from a performance standpoint and should be sealed. Shallow boreholes are comparatively insignificant and require only nominal sealing. The primary areas in which to place seals are away from high-temperature zones at a distance from the potential repository horizon in the Paintbrush nonwelded tuff and the upper portion of the Topopah Spring Member and in the tuffaceous beds of the Calico Hills Unit. Seals should be placed prior to waste emplacement. Performance goals for borehole seals both above and below the potential repository are proposed. Detailed construction information on the boreholes that could be used for future design specifications is provided along with a description of the environmental setting, i.e., the geology, hydrology, and the in situ and thermal stress states. A borehole classification scheme based on the condition of the borehole wall in different tuffaceous units is also proposed. In addition, calculations are presented to assess the significance of the boreholes acting as preferential pathways for the release of radionuclides. Design calculations are presented to answer the concerns of when, where, and how to seal. As part of the strategy development, available technologies to seal exploratory boreholes (including casing removal, borehole wall reconditioning, and seal emplacement) are reviewed.

  19. Projectables

    Rasmussen, Troels A.; Merritt, Timothy R.

    2017-01-01

    CNC cutting machines have become essential tools for designers and architects enabling rapid prototyping, model-building and production of high quality components. Designers often cut from new materials, discarding the irregularly shaped remains. We introduce ProjecTables, a visual augmented...... reality system for interactive packing of model parts onto sheet materials. ProjecTables enables designers to (re)use scrap materials for CNC cutting that would have been previously thrown away, at the same time supporting aesthetic choices related to wood grain, avoiding surface blemishes, and other...... relevant material properties. We conducted evaluations of ProjecTables with design students from Aarhus School of Architecture, demonstrating that participants could quickly and easily place and orient model parts reducing material waste. Contextual interviews and ideation sessions led to a deeper...

  20. K25/K27 Characterization for Demonstrating Criticality Incredibility K-25 / K-27 D and D Project

    Haghighi, Mahmoud H.; Howe, Kathleen E.; Chandler, John R.

    2008-01-01

    The K-25 and K-27 Buildings are currently undergoing decontamination and decommissioning (D and D) as part of a non-time critical removal action. When the enrichment process was shut-down, the process gas equipment and piping was purged to remove UF 6 , but the process gas system was not generally chemically treated to remove solid holdup. Generally, chemical treatment was completed it was not very effective in removing the larger deposits. Because of this, uranium deposits continue to reside throughout the process gas system. There is the potential for unreacted UF 6 to be present, as well. A key part of this project is to locate the remaining significant uranium deposits and manage them appropriately. Since some process gas components in the K-25 and K-27 Buildings contain enriched uranium holdup, it is important to ensure nuclear criticality safety during demolition or subsequent loading and transport of waste. Therefore, a major task in preparation for demolition is to demonstrate that 'criticality is incredible (CI)' in each building area. The U.S. Department of Energy (DOE) and Bechtel Jacobs Company LLC (BJC) are in agreement that proper and thorough characterization of fissile holdup material in the K-25 and K-27 process gas components is a key element in achieving and demonstrating that criticality is incredible, as well as in management of demolition wastes and collection of holdup material. While the classic project scheduling wisdom may indicate that performing all of the data quality objectives (DQO) and planning before the field work starts, the size of the K-25 / K-27 D and D Project combined with the evolving decommissioning plans and re-plans have indicated that a fresh look at what further characterization data are needed to meet the current project requirements. Performing a DQO in the middle of the project allowed a much greater degree of accuracy of the real project data needs than was possible even 22 months ago. The outcome of the DQO work

  1. Final Hanford Site Transuranic (TRU) Waste Characterization Quality Assurance Project Plan

    GREAGER, T.M.

    1999-01-01

    The Transuranic Waste Characterization Quality Assurance Program Plan required each US Department of Energy (DOE) site that characterizes transuranic waste to be sent the Waste Isolation Pilot Plan that addresses applicable requirements specified in the QAPP

  2. A dynamical characterization of the uncertainty in projections of regional precipitation change in the semi-arid tropics

    Giannini, A.

    2016-12-01

    The uncertainty in CMIP multi-model ensembles of regional precipitation change in tropical regions is well known: taken at face value, models do not agree on the direction of precipitation change. Consequently, in adaptation discourse, either projections are discounted, e.g., by giving more relevance to temperature projections, or outcomes are grossly misrepresented, e.g., in extrapolating recent drought into the long-term future. That this is an unsatisfactory state of affairs, given the dominant role of precipitation in shaping climate-sensitive human endeavors in the tropics, is an understatement.Here I will provide a dynamical characterization of the uncertainty in regional precipitation projections that exploits the CMIP multi-model ensembles. This characterization is based on decomposing the moisture budget and relating its terms to the influence of the oceans, specifically to the roles of moisture supply and stabilization of the vertical profile. I will discuss some preliminary findings highlighting the relevance of lessons learned from seasonal-to-interannual prediction. One such lesson is to go beyond the projection taken at face value, and understand physical processes, specifically, the role of the oceans, in order to be able to make qualitative arguments, in addition to quantitative predictions. One other lesson is to abandon the search for the "best model" and exploit the multi-model ensemble to characterize "emergent constraints".

  3. The Genomes OnLine Database (GOLD) v.5: a metadata management system based on a four level (meta)genome project classification

    Reddy, T.B.K.; Thomas, Alex D.; Stamatis, Dimitri; Bertsch, Jon; Isbandi, Michelle; Jansson, Jakob; Mallajosyula, Jyothi; Pagani, Ioanna; Lobos, Elizabeth A.; Kyrpides, Nikos C.

    2015-01-01

    The Genomes OnLine Database (GOLD; http://www.genomesonline.org) is a comprehensive online resource to catalog and monitor genetic studies worldwide. GOLD provides up-to-date status on complete and ongoing sequencing projects along with a broad array of curated metadata. Here we report version 5 (v.5) of the database. The newly designed database schema and web user interface supports several new features including the implementation of a four level (meta)genome project classification system and a simplified intuitive web interface to access reports and launch search tools. The database currently hosts information for about 19 200 studies, 56 000 Biosamples, 56 000 sequencing projects and 39 400 analysis projects. More than just a catalog of worldwide genome projects, GOLD is a manually curated, quality-controlled metadata warehouse. The problems encountered in integrating disparate and varying quality data into GOLD are briefly highlighted. GOLD fully supports and follows the Genomic Standards Consortium (GSC) Minimum Information standards. PMID:25348402

  4. Final Technical Report on STTR Project DE-FG02-06ER86281 Particle Tracking in Matter-Dominated Beam Lines (G4beamline)

    Muons, Inc.

    2011-05-19

    This project has been for software development of the G4beamline [1] program, which is a particle-tracking simulation program based on the Geant4 toolkit [2], optimized for beam lines. This program can perform more realistic simulations than most alternatives, while being significantly easier to use by physicists. This project has fostered the general acceptance of G4beamline within the muon community, and has assisted in expanding its role outside that community. During this project, the G4beamline user community has grown from about a half-dozen users to more than 200 users around the world. This project also validated our business decision to keep G4beamline an open-source program, judging that an STTR project would provide more development resources than would marketing and selling the program. G4beamline is freely available to the physics community, and has been well validated against experiments and other codes within its domain. Muons, Inc. continues to support and develop the program, and a major part of the company's continued success and growth is directly related to our expertise in applying this program to interesting applications.

  5. The Genomes OnLine Database (GOLD) v.5: a metadata management system based on a four level (meta)genome project classification

    Reddy, Tatiparthi B. K. [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Thomas, Alex D. [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Stamatis, Dimitri [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Bertsch, Jon [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Isbandi, Michelle [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Jansson, Jakob [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Mallajosyula, Jyothi [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Pagani, Ioanna [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Lobos, Elizabeth A. [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Kyrpides, Nikos C. [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); King Abdulaziz Univ., Jeddah (Saudi Arabia)

    2014-10-27

    The Genomes OnLine Database (GOLD; http://www.genomesonline.org) is a comprehensive online resource to catalog and monitor genetic studies worldwide. GOLD provides up-to-date status on complete and ongoing sequencing projects along with a broad array of curated metadata. Within this paper, we report version 5 (v.5) of the database. The newly designed database schema and web user interface supports several new features including the implementation of a four level (meta)genome project classification system and a simplified intuitive web interface to access reports and launch search tools. The database currently hosts information for about 19 200 studies, 56 000 Biosamples, 56 000 sequencing projects and 39 400 analysis projects. More than just a catalog of worldwide genome projects, GOLD is a manually curated, quality-controlled metadata warehouse. The problems encountered in integrating disparate and varying quality data into GOLD are briefly highlighted. Lastly, GOLD fully supports and follows the Genomic Standards Consortium (GSC) Minimum Information standards.

  6. In line digital holography measurement for liquid-liquid flow: application to the characterization of emulsions produced in pulsed column

    Lamadie, F.

    2013-01-01

    Several processes used in research and industry are based on liquid-liquid extraction, a method designed for selective separation of products in a mixture. In liquid-liquid extraction, two immiscible liquids are contacted: an aqueous phase and an organic phase, one of which generally contains an extractant molecule capable of transferring the desired elements to the other phase. The transfer occurs at the contact surface between the two phases. After transfer, both phases are separated by settling. In practice, these operations are performed in industrial apparatus. In order to optimize the operation of these devices, it's important to determine the fundamental characteristics of the emulsion. These include parameters related to the fluid flow velocity as well as parameters related to fluid mixing such as the interfacial area, hold-up, and size distribution of the droplets population. Numerous imaging techniques can be used to measure these parameters. One of them, digital holography, is well-known for allowing complete reconstruction of information about a 3D flow in a single shot. This PhD work deals with a direct application of digital in line holography to droplets rising in a continuous liquid phase. The droplet size imposes a regime of intermediate-field diffraction hardly explored to date. Acquired diffraction patterns show that the usual dark disk model is not valid and that good agreement is obtained with a mixed model coupling thin lens with opaque disk. Hologram focusing is nevertheless performed with a dedicated automated method. A literature review has been conducted to identify the sharpest auto-focus function for our application. In a second step, in order to measure high retention rates, an inverse problem approach is applied on all the outliers and missing droplets. This hologram restitution treatment has been applied to experimental results with a comparison to independent measurements. The main results obtained with calibrated droplets are

  7. Design of a machine to bore and line a long horizontal hole in tuff: Nevada Nuclear Waste Storage Investigations Project

    Friant, J.E.; Dowden, P.B.

    1987-09-01

    This report describes an engineering design for equipment capable of simultaneously drilling and lining deep horizontal bore holes. The ultimate use of the equipment is to bore up to 600 ft long, 3 ft diameter emplacement holes for a nuclear waste repository. The specific system designed is referred to as a Development Prototype Boring Machine (DPBM) which will be used to demonstrate the drilling/lining capability in field development tests. The system utilizes as in-hole electric drive and a vacuum chip removal and handling system. The drilling unit is capable of active directional control and uses laser-type alignment equipment. The system combines the features of a small steerable tunnel boring machine, combined with a horizontally-oriented raise drill, thereby utilizing current technology. All elements of the system are compact and mobile as required for a shaft entry, underground mining environment. 3 refs., 35 figs., 1 tab

  8. THE LICK AGN MONITORING PROJECT: BROAD-LINE REGION RADII AND BLACK HOLE MASSES FROM REVERBERATION MAPPING OF Hβ

    Bentz, Misty C.; Walsh, Jonelle L.; Barth, Aaron J.; Baliber, Nairn; Bennert, Vardha Nicola; Greene, Jenny E.; Hidas, Marton G.; Canalizo, Gabriela; Hiner, Kyle D.; Filippenko, Alexei V.; Ganeshalingam, Mohan; Lee, Nicholas; Li, Weidong; Serduke, Frank J. D.; Silverman, Jeffrey M.; Steele, Thea N.; Gates, Elinor L.; Malkan, Matthew A.; Minezaki, Takeo; Sakata, Yu

    2009-01-01

    We have recently completed a 64-night spectroscopic monitoring campaign at the Lick Observatory 3-m Shane telescope with the aim of measuring the masses of the black holes in 12 nearby (z 6 -10 7 M sun and also the well-studied nearby active galactic nucleus (AGN) NGC 5548. Nine of the objects in the sample (including NGC 5548) showed optical variability of sufficient strength during the monitoring campaign to allow for a time lag to be measured between the continuum fluctuations and the response to these fluctuations in the broad Hβ emission. We present here the light curves for all the objects in this sample and the subsequent Hβ time lags for the nine objects where these measurements were possible. The Hβ lag time is directly related to the size of the broad-line region (BLR) in AGNs, and by combining the Hβ lag time with the measured width of the Hβ emission line in the variable part of the spectrum, we determine the virial mass of the central supermassive black hole in these nine AGNs. The absolute calibration of the black hole masses is based on the normalization derived by Onken et al., which brings the masses determined by reverberation mapping into agreement with the local M BH -σ * relationship for quiescent galaxies. We also examine the time lag response as a function of velocity across the Hβ line profile for six of the AGNs. The analysis of four leads to rather ambiguous results with relatively flat time lags as a function of velocity. However, SBS 1116+583A exhibits a symmetric time lag response around the line center reminiscent of simple models for circularly orbiting BLR clouds, and Arp 151 shows an asymmetric profile that is most easily explained by a simple gravitational infall model. Further investigation will be necessary to fully understand the constraints placed on the physical models of the BLR by the velocity-resolved response in these objects.

  9. Quantitative investigation of the edge enhancement in in-line phase contrast projections and tomosynthesis provided by distributing microbubbles on the interface between two tissues: a phantom study

    Wu, Di; Donovan Wong, Molly; Li, Yuhua; Fajardo, Laurie; Zheng, Bin; Wu, Xizeng; Liu, Hong

    2017-12-01

    The objective of this study was to quantitatively investigate the ability to distribute microbubbles along the interface between two tissues, in an effort to improve the edge and/or boundary features in phase contrast imaging. The experiments were conducted by employing a custom designed tissue simulating phantom, which also simulated a clinical condition where the ligand-targeted microbubbles are self-aggregated on the endothelium of blood vessels surrounding malignant cells. Four different concentrations of microbubble suspensions were injected into the phantom: 0%, 0.1%, 0.2%, and 0.4%. A time delay of 5 min was implemented before image acquisition to allow the microbubbles to become distributed at the interface between the acrylic and the cavity simulating a blood vessel segment. For comparison purposes, images were acquired using three system configurations for both projection and tomosynthesis imaging with a fixed radiation dose delivery: conventional low-energy contact mode, low-energy in-line phase contrast and high-energy in-line phase contrast. The resultant images illustrate the edge feature enhancements in the in-line phase contrast imaging mode when the microbubble concentration is extremely low. The quantitative edge-enhancement-to-noise ratio calculations not only agree with the direct image observations, but also indicate that the edge feature enhancement can be improved by increasing the microbubble concentration. In addition, high-energy in-line phase contrast imaging provided better performance in detecting low-concentration microbubble distributions.

  10. Single Shell Tank Waste Characterization Project for Tank B-110, Core 9 - data package and PNL validation summary report

    Pool, K.N.; Jones, T.E.; McKinley, S.G.; Tingey, J.M.; Longaker, T.M.; Gibson, J.A.

    1990-01-01

    This Data Package contains results obtained by Pacific Northwest Laboratory (PNL) staff in the characterization and analyses of Core 9 segments taken from the Single-Shell Tank (SST) 110B. The characterization and analysis of Core 9 segments are outlined in the Waste Characterization Plan for Hanford Site Single-Shell Tanks and in the Pacific Northwest Laboratory (PNL) Single-Shell Tank Waste Characterization Support FY 89/90 Statement of Work (SOW), Rev. 1 dated March, 1990. Specific analyses for each sub-sample taken from a segment are delineated in Test Instructions prepared by the PNL Single-Shell Tank Waste Characterization Project Management Office (SST Project) in accordance with procedures contained in the SST Waste Characterization Procedure Compendium (PNL-MA-599). Analytical procedures used in the characterization activities are also included in PNL-MA-599. Core 9 included five segments although segment 1 did not have sufficient material for characterization. The five samplers were received from Westinghouse Hanford Company (WHC) on 11/21-22/89. Each segment was contained in a sampler and was enclosed in a shipping cask. The shipping cask was butted up to the 325-A hot cell and the sampler moved into the hot cell. The material in the sampler (i.e., the segment) was extruded from the sampler, limited physical characteristics assessed, and photographed. At this point samples were taken for particle size and volatile organic analyses. Each segment was then homogenized. Sub-samples were taken for required analyses as delineated in the appropriate Test Instruction. Table 1 includes sample numbers assigned to Core 9 segment materials being transferred from 325-A Hot Cell. Sample numbers 90-0298, 90-0299, 90-0302, and 90-0303 were included in Table 1 although no analyses were requested for these samples. Table 2 lists Core 9 sub-sample numbers per sample preparation method

  11. On-Line Ion Exchange Liquid Chromatography as a Process Analytical Technology for Monoclonal Antibody Characterization in Continuous Bioprocessing.

    Patel, Bhumit A; Pinto, Nuno D S; Gospodarek, Adrian; Kilgore, Bruce; Goswami, Kudrat; Napoli, William N; Desai, Jayesh; Heo, Jun H; Panzera, Dominick; Pollard, David; Richardson, Daisy; Brower, Mark; Richardson, Douglas D

    2017-11-07

    Combining process analytical technology (PAT) with continuous production provides a powerful tool to observe and control monoclonal antibody (mAb) fermentation and purification processes. This work demonstrates on-line liquid chromatography (on-line LC) as a PAT tool for monitoring a continuous biologics process and forced degradation studies. Specifically, this work focused on ion exchange chromatography (IEX), which is a critical separation technique to detect charge variants. Product-related impurities, including charge variants, that impact function are classified as critical quality attributes (CQAs). First, we confirmed no significant differences were observed in the charge heterogeneity profile of a mAb through both at-line and on-line sampling and that the on-line method has the ability to rapidly detect changes in protein quality over time. The robustness and versatility of the PAT methods were tested by sampling from two purification locations in a continuous mAb process. The PAT IEX methods used with on-line LC were a weak cation exchange (WCX) separation and a newly developed shorter strong cation exchange (SCX) assay. Both methods provided similar results with the distribution of percent acidic, main, and basic species remaining unchanged over a 2 week period. Second, a forced degradation study showed an increase in acidic species and a decrease in basic species when sampled on-line over 7 days. These applications further strengthen the use of on-line LC to monitor CQAs of a mAb continuously with various PAT IEX analytical methods. Implementation of on-line IEX will enable faster decision making during process development and could potentially be applied to control in biomanufacturing.

  12. Establishment and characterization of a new cell line, FPS-1, derived from human undifferentiated pleomorphic sarcoma, overexpressing epidermal growth factor receptor and cyclooxygenase-2.

    Hakozaki, Michiyuki; Hojo, Hiroshi; Sato, Michiko; Tajino, Takahiro; Yamada, Hitoshi; Kikuchi, Shinichi; Abe, Masafumi

    2006-01-01

    Undifferentiated pleomorphic sarcoma (UPS) is among the most common soft tissue sarcomas in adults. In order to improve its aggressive course or prognosis and establish new therapeutic methods, molecular genetic and biological characterizations of UPS are required. A new human UPS cell line (FPS-1) was established from UPS of the upper arm of a 79-year-old man. The cell line has been maintained for over 14 months with more than 60 passages. FPS-1 cells were characterized using molecular biological methods. FPS-1 cells showed the same morphological and immunophenotypical characteristics as the primary tumor. Cytogenetic and molecular analyses revealed a nonsense mutation in exon 6 of the p53 gene. Epidermal growth factor receptor (EGFR) and cyclooxygenase-2 (COX-2) were expressed in FPS-1 cells. FPS-1 cells might be useful for investigating biological behavior and developing new molecular targeting antitumor drugs for UPS with EGFR or COX-2 expression.

  13. Generation, genome edition and characterization of iPSC lines from a patient with coenzyme Q10 deficiency harboring a heterozygous mutation in COQ4 gene

    Damià Romero-Moya

    2017-10-01

    Full Text Available We report the generation, CRISPR/Cas9-edition and characterization of induced pluripotent stem cell (iPSC lines from a patient with coenzyme Q10 deficiency harboring the heterozygous mutation c.483G > C in the COQ4 gene. iPSCs were generated using non-integrative Sendai Viruses containing the reprogramming factors OCT4, SOX2, KLF4 and C-MYC. The iPSC lines carried the c.483G > C COQ4 mutation, silenced the OKSM expression and were mycoplasma-free. They were bona fide pluripotent cells as characterized by morphology, immunophenotype/gene expression for pluripotent-associated markers/genes, NANOG and OCT4 promoter demethylation, karyotype and teratoma formation. The COQ4 mutation was CRISPR/Cas9 edited resulting in isogenic, diploid and off-target free COQ4-corrected iPSCs.

  14. Identification and characterization of conservative organic tracers for use as hydrologic tracers for the Yucca Mountain site characterization project: Quality Assurance Project Plan, Revision 1

    Stetzenbach, K.J.

    1993-01-01

    The purpose of this work is to identify and characterize candidate conservative organic tracers for use as hydrologic tracers for experiments to be conducted at the Yucca Mountain C-well complex. During this quarter the main effort was directed towards rewriting the quality assurance program in preparation for a review and audit by the USGS. However, due to budget constraints the review and audit were not carried out. The tracer QA plan and standard operating procedures (SOPs) were revised and copies are included in the report. Instrumental problems were encountered and corrected with the addition of new integration and sample control software. In the sampling, there was an unexplained peak in the chromatograms of the tracers being tested in the light tuff. This was not correctable and these experiments will be repeated in the next quarter

  15. Derivation, Characterization, and Neural Differentiation of Integration-Free Induced Pluripotent Stem Cell Lines from Parkinson's Disease Patients Carrying SNCA, LRRK2, PARK2, and GBA Mutations

    Momcilovic, Olga; Sivapatham, Renuka; Oron, Tal Ronnen

    2016-01-01

    We report generation of induced pluripotent stem cell (iPSC) lines from ten Parkinson's disease (PD) patients carrying SNCA, PARK2, LRRK2, and GBA mutations, and one age-matched control. After validation of pluripotency, long-term genome stability, and integration-free reprogramming, eight...... not be sufficient to determine the cause or mechanism of the disease, and highlights the need to use more focused strategies for large-scale data analysis........ We further examined gene expression in a stress model (MPTP-induced dopaminergic neuronal death) using two clones from the SNCA triplication line, and detected changes in genes associated with mitophagy. Our data suggested that even a well-characterized line of a monogenic disease may...

  16. Characterization of Camptothecin-induced Genomic Changes in the Camptothecin-resistant T-ALL-derived Cell Line CPT-K5

    Kjeldsen, Eigil; Nielsen, Christine J F; Roy, Amit

    2018-01-01

    -K5 and its parental cell line. We identified copy number alterations affecting genes important for maintaining genome integrity and reducing CPT-induced DNA damage. We show for the first time that short tandem repeats are targets for TOP1 cleavage, that can be differentially stimulated by CPT.......Acquisition of resistance to topoisomerase I (TOP1)-targeting camptothecin (CPT) derivatives is a major clinical problem. Little is known about the underlying chromosomal and genomic mechanisms. We characterized the CPT-K5 cell line expressing mutant CPT-resistant TOP1 and its parental T......-cell derived acute lymphoblastic leukemia CPT-sensitive RPMI-8402 cell line by karyotyping a