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Sample records for lepeletier hymenoptera apidae

  1. Diferenciação genética entre Melipona mondury, Smith 1863, Melipona rufiventris, Lepeletier, 1836 e Melipona sp. (Hymenoptera, Apidae) no estado de Minas Gerais, Brasil, utilizando marcadores ISSR

    OpenAIRE

    Dias, Fábia Guimarães

    2008-01-01

    Melipona rufiventris, Lepeletier, 1836 e M. mondury, Smith, 1863 (Hymenoptera, Apidae) são espécies geneticamente similares, popularmente conhecidas como uruçu amarela. Estudos recentes, utilizando marcadores moleculares mostraram que as populações de uruçu amarela em Minas Gerais formam grupos distintos, sendo M. mondury pertencente à região de Mata Atlântica, M. rufiventris e uma terceira espécie, ainda não identificada aqui denominada de Melipona sp., pertencentes à região do Cerrado. O ob...

  2. Morphological and Chemical Characterization of the Invasive Ants in Hives of Apis mellifera scutellata Lepeletier (Hymenoptera: Apidae).

    Science.gov (United States)

    Simoes, M R; Giannotti, E; Tofolo, V C; Pizano, M A; Firmino, E L B; Antonialli-Junior, W F; Andrade, L H C; Lima, S M

    2016-02-01

    Apiculture in Brazil is quite profitable and has great potential for expansion because of the favorable climate and abundancy of plant diversity. However, the occurrence of pests, diseases, and parasites hinders the growth and profitability of beekeeping. In the interior of the state of São Paulo, apiaries are attacked by ants, especially the species Camponotus atriceps (Smith) (Hymenoptera: Formicidae), which use the substances produced by Apis mellifera scutellata (Lepeletier) (Hymenoptera: Apidae), like honey, wax, pollen, and offspring as a source of nourishment for the adult and immature ants, and kill or expel the adult bees during the invasion. This study aimed to understand the invasion of C. atriceps in hives of A. m. scutellata. The individuals were classified into castes and subcastes according to morphometric analyses, and their cuticular chemical compounds were identified using Photoacoustic Fourier transform infrared spectroscopy (FTIR-PAS). The morphometric analyses were able to classify the individuals into reproductive castes (queen and gynes), workers (minor and small ants), and the soldier subcaste (medium and major ants). Identification of cuticular hydrocarbons of these individuals revealed that the eight beehives were invaded by only three colonies of C. atriceps; one of the colonies invaded only one beehive, and the other two colonies underwent a process called sociotomy and were responsible for the invasion of the other seven beehives. The lack of preventive measures and the nocturnal behavior of the ants favored the invasion and attack on the bees.

  3. Higher-level bee classifications (Hymenoptera, Apoidea, Apidae sensu lato Classificação dos grandes grupos de abelhas (Hymenoptera, Apoidea, Apidae sensu lato

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    Gabriel A. R. Melo

    2005-03-01

    Full Text Available A higher-level classification of bees, in which the entire group is treated as a single family - the Apidae - is advocated here. A total of seven subfamilies, 51 tribes and 27 subtribes are recognized. These subfamilies correspond to the families adopted in the traditional classification. Although the proposed changes do not involve any major rearrangement, basically only changing the rank given to the main groups, the new system makes the classification of bees more consistent with that adopted for other major groups of aculeate Hymenoptera. It also departs from the 19th century practice, perpetuated in the traditional classification, of giving family-status to the main groups of bees. A correspondence table associating the taxon names used in the current traditional classification with those of the revised classification is presented. Scrapterini new tribe (type-genus Scrapter Lepeletier & Serville is proposed to accommodate the southern African genus Scrapter.Apresenta-se uma classificação para as abelhas em que o todo o grupo é tratado como uma única família - Apidae. São reconhecidas sete subfamílias, 51 tribos e 27 subtribos. As subfamílias correspondem às famílias da classificação tradicional. Apesar das mudanças propostas afetarem apenas o status dos grupos, o novo sistema torna a classificação das abelhas mais consistente com aquela adotada para os grandes grupos de Hymenoptera aculeados. Além disso, distancia-se da tradição de dar status de família aos grupos principais de abelhas, uma prática do século 19 perpetuada na classificação tradicional. É apresentada uma tabela de correspondência associando os nomes dos táxons usados na classificação tradicional corrente com aquelas da classificação sendo proposta aqui. Scrapterini tribo nova (gênero-tipo Scrapter Lepeletier & Serville é proposta para acomodar Scrapter, um gênero restrito à porção sul do continente africano.

  4. CARACTERIZAÇÃO FÍSICO-QUÍMICA DO MEL DE MELIPONA FULVA LEPELETIER, 1836 (HIMENOPTERA: APIDAE: MELIPONINAE) UTILIZADA NA MELIPONICULTURA POR COMUNIDADES TRADICIONAIS DO ENTORNO DA CIDADE DE MACAPÁ-AP

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    Chaves, Alison Felipe Alencar; Gomes, Jorge Emílio Henriques; da Costa, Arley José Silveira

    2012-01-01

    O estudo buscou determinar as características físico-químicas do mel de abelha sem ferrão Melipona fulva Lepeletier, 1836 (Hymenoptera: Apidae: Meliponinae) utilizada na meliponicultura por comunidades tradicionais do entorno da cidade de Macapá, AP. As características físico-químicas analisadas foram: umidade, cinzas, acidez, pH, condutividade e hidroximetilfurfural. Os resultados foram expressos em média ± desvio padrão e comparados com os valores limites presentes na legislação vigente e c...

  5. Morphometry of the midgut of Melipona quadrifasciata anthidioides (Lepeletier) (Hymenoptera: Apidae) during metamorphosis.

    Science.gov (United States)

    Cruz, L C; Araújo, V A; Dolder, H; Araújo, A P A; Serrão, J E; Neves, C A

    2011-01-01

    In Hymenoptera, midgut changes begin in the last instar. At this stage, the larval epithelial digestive cells degenerate, leaving only the basal membrane and the regenerative cells which will develop into a new epithelium during the pupal stage and in the adult. Epithelium renewal is followed by changes in volume and shape of the midgut. Morphometric analysis of digestive cells and total midgut volume of Melipona quadrifasciata anthidioides (Lepeletier) were conducted to verify whether cell volume increase are sufficient to account for the total midgut volume increase that occurs during metamorphosis. An increase in midgut volume was verified in spite of the scarcity of cell proliferation found during metamorphosis. At the end of metamorphosis, the increase in cell volume was not sufficient to explain the increase in volume of the midgut, indicating that an increase in the number of digestive cells is apparently necessary. Nevertheless, the mechanism by which regenerative cells reconstitute the epithelium during metamorphosis remains unknown.

  6. Enkele bijzondere bijenwaarnemingen (Hymenoptera: Apidae)

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    Raemakers, I.P.

    2000-01-01

    Some interesting records on Dutch bees (Hymenoptera: Apidae) The publication of the preliminary atlas of Dutch bees (Peeters et al. 1999) has stimulated many specialists in their mapping activity. The author reports several interesting new distribution records on ten bee-species in 1999. The Dutch

  7. De wespbijen (Nomada) van Nederland (Hymenoptera: Apidae)

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    Smit, J.

    2004-01-01

    The wasp bees (Nomada) of the Netherlands (Hymenoptera: Apidae) In the Netherlands 44 species of Nomada are known to occur. The name wasp bee is derived from the often yellow and black colour, which give the insects a wasplike appearance. Nomada bees are cleptoparasites mostly with Andrena species.

  8. Effects of Apis mellifera adansonii, L. 1758 (Apidae: Hymenoptera ...

    African Journals Online (AJOL)

    Effects of Apis mellifera adansonii, L. 1758 (Apidae: Hymenoptera) pollination on yields of Cucumeropsis mannii (Naudin) in Kisangani, Democratic Republic of Congo. Boniface Posho Ndola, Yves Brostaux, Guillaume Le Goff, Marie-Lucie Susini, Eric Haubruge, Frederic Francis, Bach Kim Nguyen ...

  9. Community of orchid bees (Hymenoptera: Apidae in transitional vegetation between Cerrado and Atlantic Forest in southeastern Brazil

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    EP. Pires

    Full Text Available The community of orchid bees (Hymenoptera: Apidae: Euglossina was studied at an area in the transition between the Cerrado and Atlantic Forest biomes, from March, 2010 to February, 2011 in the Barroso region, state of Minas Gerais, eastern Brazil. Orchid-bee males were collected with bait traps containing three different scents (cineole, eugenol and vanillin and with entomological nets for collecting bees on flowers. A total of 614 orchid-bee males were collected using aromatic traps, belonging to four genera and 15 species. Twenty-five female specimens belonging to two genera and at least three species were collected on flowers. Eulaema (Apeulaema nigrita Lepeletier, 1841 was the most abundant species (50% of collected specimens, followed by Euglossa (Euglossa truncata Rebêlo & Moure, 1996 (28%. Cineole was the most attractive compound (66.5% of males and 13 species, followed by eugenol (16% and 9 species and vanillin (13.5% and 4 species. Eulaema (Apeulaema marcii Nemésio, 2009 and Eufriesea auriceps (Friese, 1899 were attracted to all scents, whereas Euglossa species were collected only in cineole and eugenol.

  10. Bombus brasiliensis Lepeletier (Hymenoptera, Apidae infected with Nosema ceranae (Microsporidia

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    Santiago Plischuk

    Full Text Available ABSTRACT Heavy infections caused by a microsporidium were detected in midgut epithelium cells of two adult workers of the bumble bee Bombus brasiliensis Lepeletier collected near Puerto Iguazú, Misiones province, Argentina. Microsporidium rRNA (16S small subunit was amplified by 218MITOC primers and produced amplicons indicating presence of Nosema ceranae Fries et al., a virulent pathogen of more than 20 bee species, possibly involved in Apis mellifera L. Colony Collapse Disorder. Campaigns in search of B. brasiliensis between 2008 and 2015 have revealed a possible narrower range in the southeastern area of its known distribution. Effects of N. ceranae infections could be modulating their populations and should not be overlooked. In addition, the wide host range of this microsporidium makes it a potential threat to several endemic bees such as stingless (Meliponini and orchid bees (Euglossini.

  11. De kortsnuitbloedbij Sphecodes majalis nieuw voor de Nederlandse fauna (Hymenoptera: Apidae)

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    Raemakers, I.

    2004-01-01

    Sphecodes majalis, a new bee species for the Netherlands (Hymenoptera: Apidae) A population of Sphecodes majalis was found on a limestone grassland near Maastricht (Limburg). On several occasions more than 10 female and several male specimen were observed. Sphecodes majalis is a parasite of

  12. Espécies novas de Trigonisca Moure (Hymenoptera, Apidae, Apinae New species of Trigonisca Moure (Hymenoptera, Apidae, Apinae

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    Patrícia Maia Correia de Albuquerque

    2007-01-01

    Full Text Available Algumas espécies novas de Meliponini do gênero Trigonisca Moure, 1950 (Hymenoptera, Apidae, Apinae do Brasil e Panamá são descritas: T. roubiki sp. nov. (Panamá, T. variegatifrons sp. nov. (Brasil: RO, PA, MT, T. vitrifrons sp. nov. (Brasil: AM, PA, T. unidentata sp. nov. (Brasil: AM, T. meridionalis sp. nov. (Brasil: PA, MA, MT, MG, SP, T. bidentata sp. nov. (Brasil: RO, T. extrema sp. nov. (Brasil: AM e T. hirticornis sp. nov. (Brasil: RO, PA; e são apresentados novos registros geográficos de T. flavicans (Moure, 1950, T. intermedia Moure, 1989, T. dobzhanskyi (Moure, 1950, T. ceophloei (Schwarz, 1938, T. nataliae (Moure, 1950 e T. pediculana (Fabricius, 1804 e uma chave para identificação das espécies.Some new Meliponini species of the genus Trigonisca Moure, 1950 (Hymenoptera, Apidae, Apinae from Brazil and Panamá are described: T. roubiki sp. nov. (Panamá, T. variegatifrons sp. nov. (Brazil: RO, PA, MT, T. vitrifrons sp. nov. (Brazil: AM, PA, T. unidentata sp. nov. (Brazil: AM, T. meridionalis sp. nov. (Brazil: PA, MA, MT, MG, SP, T. bidentata sp. nov. (Brazil: RO, T. extrema sp. nov. (Brazil: AM and T. hirticornis sp. nov. (Brazil: RO, PA; additional geographic records from T. flavicans (Moure, 1950, T. intermedia Moure, 1989, T. dobzhanskyi (Moure, 1950, T. ceophloei (Schwarz, 1938, T. nataliae (Moure, 1950 and T. pediculana (Fabricius, 1804 and an identification key for species are presented.

  13. Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae Enzymatic polymorphism in Melipona quadrifasciata anthidioides Lepeletier populations (Hymenoptera, Apidae, Meliponinae

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    Davi S. Aidar

    2001-06-01

    Full Text Available Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52 were prepared for starch gel electrophoresis in order to investigate the genetic variation of the following enzimes: esterase (EST, isocitrate dehydrogenase (IDH, malic enzyme (ME, phosphoglucomutase (PGM, superoxide desmutase (SOD, α-glycerophosphate dehydrogenase (αPGD, malate dehydrogenase (MDH, leucine aminopeptidase (LAP, hexokinase (HK and phosphoglucose isomerase (PGI. The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles(est-sand and est-f while all the others were shown to be monomorphic. The allele EST-S showed a frequency of 82,6%.

  14. On the identity of Melipona torrida Friese (Hymenoptera, Apidae)

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    Melo, Gabriel A. R.

    2013-01-01

    On the identity of Melipona torrida Friese (Hymenoptera, Apidae). Melipona marginata var. torrida Friese, 1916, described from three workers putatively collected in Costa Rica, never had its identity properly recognized. Since its original description, no additional specimens have ever been collected in Costa Rica. It is argued here that Melipona torrida was based on mislabeled specimens and corresponds to Melipona marginata obscurior Moure, 1971, a form known only from southern Brazil, Argen...

  15. Nesting biology of Centris (Centris aenea Lepeletier (Hymenoptera, Apidae, Centridini

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    Cândida Maria Lima Aguiar

    2003-12-01

    Full Text Available Nesting activity of Centris aenea Lepeletier, 1841 was studied in two Brazilian habitats, Caatinga (Monte Santo, Bahia and Cerrado (Palmeiras, Bahia and Luiz Antônio, São Paulo. Nests were excavated in the ground and did not tend to be aggregated together at the two sites, but at Palmeiras, nests were in a large aggregation. Nest architecture consists of a single unbranched tunnel, sloping to vertical, which leads to a linear series of four cells, placed from 8 to 26 cm in depth. Cells are urn-shaped with a rounded base, and their cell caps have a central hollow process, as in other Centridini. Nest architecture of C. aenea was compared to other species of Centris Fabricius, 1804. Provisions are composed of a pollen mass covered by a thin liquid layer on which the egg is placed. Females were observed gathering oil on Mcvaughia bahiana W.R. Anderson flowers from October to March in the Caatinga, and on Byrsonima intermedia A.Juss. as well as other Malpighiaceae species from August to December in the Cerrado. Pollen is gathered by buzzing flowers of Solanaceae, Caesalpiniaceae, Malpighiaceae, and Ochnaceae. Several nectar sources were recorded. There is indirect evidence that Mesoplia sp. parasitizes nests of C. aenea in the Cerrado.

  16. Biologia de nidificação de Centris (Hemisiella trigonoides Lepeletier (Hymenoptera, Apidae, Centridini Nesting biology of Centris (Hemisiella trigonoides Lepeletier (Hymenoptera, Apidae, Centridini

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    Cândida M. L. Aguiar

    2006-06-01

    Full Text Available O comportamento de nidificação de Centris (Hemisiella trigonoides Lepeletier, 1841, e o comportamento de seus cleptoparasitas foram estudados em Monte Santo, Bahia, Brasil. As abelhas construíram seus ninhos com uma mistura de solo e óleo, dentro de cavidades preexistentes na madeira de uma construção abandonada, assim como em gomos de bambu de 8 e 9 mm de diâmetro. Os ninhos completados tinham de uma a cinco células alongadas, arranjadas em uma série linear e orientadas horizontalmente. O tempo gasto para construir uma célula foi altamente variável, sendo em geral de 4,5 a 5,5 h. Após finalizar a construção da célula, as fêmeas fizeram uma ou duas viagens para coletar um líquido incolor, provavelmente óleo floral, usado para revestir as paredes internas da célula. Para aprovisionar uma célula foram realizadas de cinco a oito viagens de coleta para obtenção de pólen e néctar, e de quatro a seis viagens para coleta de óleo. Imediatamente após a oviposição, as fêmeas fecharam as células usando o solo que elas tinham coletado previamente. Três espécies cleptoparasitas pertencentes ao gênero Coelioxys Latreille, 1809 atacaram os ninhos. Entradas de cleptoparasitas dentro dos ninhos occorreram, na maioria dos casos, enquanto a fêmea hospedeira estava ausente do ninho. As fêmeas de C. (H. trigonoides apresentaram comportamentos defensivos para evitar parasitismo, tais como expulsar os parasitas e guardar os ninhos. Machos de C. (H. trigonoides usaram o local de nidificação como abrigo durante as horas mais quentes do dia, assim como para dormir. Eles deixavam as cavidades no dia seguinte entre 09:00 e 10:30 h. Isto sugere que machos e fêmeas têm padrões temporais de atividade distintos.The nesting behavior of Centris (Hemisiella trigonoides Lepeletier, 1841, and the behavior of their cleptoparasites were studied at Monte Santo, Bahia, Brazil. The females constructed their nests within preexisting holes in wood

  17. Comunidade de Euglossini (Hymenoptera, Apidae das dunas litorâneas do Abaeté, Salvador, Bahia, Brasil

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    Blandina Felipe Viana

    2002-01-01

    Full Text Available Community of Euglossini (Hymenoptera, Apidae from the coastal sand dunes of Abaeté, Salvador, Bahia, Brazil. The Euglossini community structure was analyzed by attracting males with the scents eucalyptol, eugenol, vanillin, benzyl benzoate and methyl salicylate, and by netting bees on flowers. The samplings took place three times a month along one year from 6:00 a.m. to 6:00 p.m. The scent baits attracted 670 individuals belonging to seven species of three genus. The predominant species were Euglossa cordata (Linnaeus, 1758 (76.6% and Eulaema nigrita Lepeletier, 1841 (21.8%. Euglossini males visited the scents along the whole year, being more abundant in May and in August. The most efficient fragrance was eucalyptol, attracting 624 individuals of five species. The males abundance fluctuated along the day, being the highest frequency observed between 8:00 a.m. to 10:00 a.m. Forty eigth Euglossini females of four species were netted visiting flowers of 14 plant species belonging to 13 families. Solanaceae and Caesalpiniaceae were the most visited. The species catched on flowers were Euglossa cordata, Eulaema nigrita, Euplusia mussitans (Fabricius, 1787 and Eulaema meriana flavescens Friese 1899. Euglossa cordata was the predominant species on flowers (64.6%, being collected during almost the whole year. Euplusia mussitans was the only species netted on flowers which males were not sampled on the scents.

  18. Bumble bee (Hymenoptera: Apidae) community structure on two sagebrush steppe sites in southern Idaho

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    Stephen P. Cook; Sara M. Birch; Frank W. Merickel; Carrie Caselton Lowe; Deborah Page-Dumroese

    2011-01-01

    Although sagebrush, Artemisia spp., does not require an insect pollinator, there are several native species of bumble bees, Bombus spp. (Hymenoptera: Apidae), that are present in sagebrush steppe ecosystems where they act as pollinators for various forbs and shrubs. These native pollinators contribute to plant productivity and reproduction. We captured 12 species of...

  19. Predation of Apiomerus pilipes (Fabricius (Hemiptera, Reduviidae, Harpactorinae, Apiomerini over Meliponinae bees (Hymenoptera, Apidae, in the State of Amazonas, Brazil

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    Alexandre Coletto da Silva

    2004-12-01

    Full Text Available The present work shows the occurrence of an intense predatory activity on adults working Meliponinae bees (Hymenoptera, Apidae, by Apiomerus pilipes (Fabricius, 1787 (Hemiptera, Reduviidae, Harpactorinae, Apiomerini at a meliponary in the Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas State, Brazil.O presente trabalho registra a ocorrência de intensa atividade predatória de Apiomerus pilipes (Fabricius, 1787 (Hemiptera, Reduviidae, Harpactorini, Apiomerini sobre operárias adultas de meliponíneos (Hymenoptera, Apidae, no meliponário experimental do Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Estado do Amazonas, Brasil. O meliponário se encontra num fragmento de vegetação secundária no próprio INPA.

  20. Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae by fluorochromes

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    Jefferson de Brito Marthe

    2010-01-01

    Full Text Available Four colonies of the stingless bee Partamona cupira (Hymenoptera: Apidae were cytogenetically analyzed using conventional staining and the fluorochromes CMA3 e DAPI. The females have 2n = 34 chromosomes (2K=32+2. Some females, however, presented an additional large B acrocentric chromosome, to a total of 2n = 35. Chromosome B and the chromosomal pairs 2, 9 and 10 showed CMA3+ bands, indicating an excess of CG base-pairs. A clear association was verified between the P. helleri B chromosome SCAR marker and the presence of a B chromosome in P. cupira. The data obtained suggests that B chromosomes in P. helleri and P. cupira share a common origin.

  1. Meliponini neotropicais: o gênero Ptilotrigona Moure (Hymenoptera, Apidae, Apinae

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    João M. F. Camargo

    2004-01-01

    Full Text Available O gênero neotropical de abelhas sem ferrão, Ptilotrigona Moure, 1951, é revisado. Três espécies são reconhecidas: Ptilotrigona occidentalis (Schulz, 1904, endêmica do NW da América do Sul - do NW do Equador até o sul de Darién -, e com uma população isolada na Península de Osa - Costa Rica; P. pereneae (Schwarz, 1943, endêmica do oeste da Amazônia, e P. lurida (Smith, 1854, amplamente distribuída na Amazônia. Ptilotrigona lurida e P. pereneae são as únicas abelhas sem ferrão que estocam pólen em associação com leveduras (Candida sp. e produzem pouco ou nenhum mel. Ninhos são descritos e ilustrados. Holótipos de Trigona suffragata Cockerell, 1922 (sin. de P. occidentalis e Trigona manni Cockerell, 1912, e exemplares de Trigona heideri Friese, 1900 (sins. de P. lurida, identificados por Friese, e um parátipo de Trigona (Tetragona heideri pereneae Schwarz, 1943, são estudados. Novo sinônimo: Ptilotrigona lurida (Smith, 1854 = Trigona mocsaryi lutea Friese, 1903 syn. nov. Na análise cladística, espécies de Camargoia Moure, 1989, e Tetragona Lepeletier & Serville, 1828, foram incluídas como grupos externos; a hipótese apresentada é a seguinte: ((((Ptilotrigona lurida, P. pereneae P. occidentalis((Camargoia nordestina, C. pilicornis C. camargoi Tetragona goettei. Uma chave de identificação para as espécies e outros aspectos bionômicos também são apresentados.Neotropical Meliponini: the genus Ptilotrigona Moure, (Hymenoptera, Apidae, Apinae. The Neotropical stingless bees genus Ptilotrigona Moure, 1951 is revised. Three species are recognized: Ptilotrigona occidentalis (Schulz, 1904, endemic to NW South America - from NW Ecuador to southern Darién -, and with one isolated population in Osa Peninsula - Costa Rica; P. pereneae (Schwarz, 1943, endemic to the western Amazon; and P. lurida (Smith, 1854, largely distributed in the Amazon region. Ptilotrigona lurida and P. pereneae are the only known stingless bees

  2. A new species of Eufriesea Cockerell (Hymenoptera, Apidae, Euglossina from northeastern Brazil

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    Luiz R. R. Faria

    2011-03-01

    Full Text Available A new species of Eufriesea Cockerell (Hymenoptera, Apidae from northeastern Brazil. Eufriesea pyrrhopyga sp. nov. a short-tongued Eufriesea is described as a new species. It can be easily recognized for its predominantly violet lower frons and thorax, violet tergum 1 contrasting with the strong reddish coloration on the lateral portions of terga 2 to 4 and on entire terga 5 and 6, and head pubescence with contrasting colors, white on the lower two-thirds of the face and black on upper frons and vertex. This new species, collected in Recife (Pernambuco, Brazil, apparently is restricted to the Pernambuco endemic center, and seems to be highly endangered.

  3. Contribution to Taxonomy and Distribution of the Genus Elaphropoda (Hymenoptera: Apidae: Apinae in Vietnam

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    Nguyen, Minh Phuong

    2016-04-01

    Full Text Available Taxonomic notes on the genus Elaphropoda Lieftinck, 1966 (Hymenoptera: Apidae from Vietnam are presented. Two species of the genus are reported: Elaphropoda percarinata (Cockerell, 1930 was first recorded from Vietnam based on specimens collected from Ha Tinh province in 1998, and is reconfirmed with a specimens collected from Bac Kan province in the Northeastern part of the country in this study, and Elaphropoda khasiana (Schulz, 1906 is recorded from Vietnam for the first time. Redescriptions of the male of E. percarinata and the female of E. khasiana are given with illustrations.

  4. Melostelis gen. nov., espécies novas e notas complementares sobre Anthidiini (Hymenoptera, Apidae

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    Danúncia Urban

    2011-06-01

    Full Text Available Melostelis gen. nov., espécies novas e notas complementares sobre Anthidiini (Hymenoptera, Apidae. Melostelis gen. nov. é proposto para um novo Anthidiini cleptoparasita. São descritas e ilustradas duas espécies novas: Melostelis amazonensis sp. nov. de Manaus, Amazonas e Larocanthidium chacoense sp. nov. de Porto Murtinho, Mato Grosso do Sul. São dados a conhecer os machos de Epanthidium bolivianum Urban, 1995 e Epanthidium araranguense Urban, 2006 e, registrados pela primeira vez no Brasil, na sub-região do chaco, Ketianthidium zanolae Urban, 2000 e Epanthidium bolivianum.

  5. On the identity of Melipona torrida Friese (Hymenoptera, Apidae

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    Gabriel A. R. Melo

    2013-09-01

    Full Text Available On the identity of Melipona torrida Friese (Hymenoptera, Apidae. Melipona marginata var. torrida Friese, 1916, described from three workers putatively collected in Costa Rica, never had its identity properly recognized. Since its original description, no additional specimens have ever been collected in Costa Rica. It is argued here that Melipona torrida was based on mislabeled specimens and corresponds to Melipona marginata obscurior Moure, 1971, a form known only from southern Brazil, Argentina and Paraguay. A lectotype is designated for Melipona torrida and notes on the type material of Melipona marginata obscurior are provided. Other known examples of species described from mislabeled specimens in Friese's Zur Bienenfauna von Costa Rica are discussed. It is pointed out that additional names proposed in this work, based on material from Costa Rica, might turn out to correspond to South American taxa. Also, the date of publication of this Friese's paper is discussed.

  6. Notes on the systematics of the orchid-bee genus Eulaema (Hymenoptera, Apidae

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    Gabriel A. R. Melo

    2014-09-01

    Full Text Available Notes on the systematics of the orchid-bee genus Eulaema (Hymenoptera, Apidae. The classification of the genus Eulaema is modified in order to make it congruent with recent phylogenetic hypotheses based on molecular data. The speciosa group, containing E. peruviana, E. speciosa and related species, is removed from E. (Eulaema and transferred to E. (Apeulaema. New morphological characters are presented to support the revised scope of the subgenera and their diagnostic features are revised. Six species groups are recognized herein: two in E. (Apeulaema and four in E. (Eulaema. A list of valid species in each species group and an identification key to males of each of the subgenera and species groups are provided. Finally, an older overlooked designation of a type species for Eulaema is presented in the Appendix.

  7. Antibacterial Compounds from Propolis of Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae) from Thailand

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    Sanpa, Sirikarn; Popova, Milena; Bankova, Vassya; Tunkasiri, Tawee; Eitssayeam, Sukum; Chantawannakul, Panuwan

    2015-01-01

    This study investigated the chemical composition and antimicrobial activity of propolis collected from two stingless bee species Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae). Six xanthones, one triterpene and one lignane were isolated from Tetragonula laeviceps propolis. Triterpenes were the main constituents in T. melanoleuca propolis. The ethanol extract and isolated compounds from T. laeviceps propolis showed a higher antibacterial activity than those of T. melanoleuca propolis as the constituent α-mangostin exhibited the strongest activity. Xanthones were found in propolis for the first time; Garcinia mangostana (Mangosteen) was the most probable plant source. In addition, this is the first report on the chemical composition and bioactivity of propolis from T. melanoleuca. PMID:25992582

  8. Antibacterial Compounds from Propolis of Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae from Thailand.

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    Sirikarn Sanpa

    Full Text Available This study investigated the chemical composition and antimicrobial activity of propolis collected from two stingless bee species Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae. Six xanthones, one triterpene and one lignane were isolated from Tetragonula laeviceps propolis. Triterpenes were the main constituents in T. melanoleuca propolis. The ethanol extract and isolated compounds from T. laeviceps propolis showed a higher antibacterial activity than those of T. melanoleuca propolis as the constituent α-mangostin exhibited the strongest activity. Xanthones were found in propolis for the first time; Garcinia mangostana (Mangosteen was the most probable plant source. In addition, this is the first report on the chemical composition and bioactivity of propolis from T. melanoleuca.

  9. No Evidence of Habitat Loss Affecting the Orchid Bees Eulaema nigrita Lepeletier and Eufriesea auriceps Friese (Apidae: Euglossini) in the Brazilian Cerrado Savanna.

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    Silva, D P; De Marco, P

    2014-12-01

    Habitat loss, landscape fragmentation, and agriculture intensification constitute the main threats to bees. As the organisms responsible for almost one third of the food produced worldwide, there are growing concerns on bees' response to human-related disturbances. Among all bee groups, orchid bees (Apidae: Euglossini) compose an interesting group to test landscape-related hypotheses. In here, we tested the effect of landscape features (amount of anthropic areas and isolation) on the probability of occurrence and the abundances of both Eulaema nigrita Lepeletier and Eufriesea auriceps Friese in the Cerrado savanna. In general, we did not observe any effect of landscape features on the probability of occurrence and abundances of both species in our sampling sites. Given their potential high dispersal abilities, these bee species may be less sensitive to fragmented landscapes or even positively affected by the increase of anthropic habitats. Since we sampled many E. nigrita specimens in highly preserved Cerrado savanna areas, we believe that at least for this biome, this species may not be a good indicator of landscape disturbance.

  10. Bees (Hymenoptera: Apidae present in the flowers of the balsa wood Ochroma lagopus Swartz, 1788 = Abelhas (Hymenoptera: Apidae associadas às flores do pau-de-balsa Ochroma lagopus Swartz, 1788

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    Carla Regina Guimarães Brighenti

    2010-10-01

    Full Text Available The flower of balsa wood holds about 10 to 15 mL of nectar, which helps attracting pollinating agents, since the genus Ochroma is incapable of self-fertilization. However, a high mortality of bees is observed in these flowers. The present study investigated the frequency and constancy of mortality of the individuals of the familyApidae that fed on nectar from the balsa wood. Data was gathered from June to August 2008, in Lavras – Minas Gerais State, Brazil. In addition, the survival of the Africanized bees that fed on the nectar of this flower was compared to those that fed on 50% aqueous solution of honey. Forty flowers were analyzed, and 949 individuals of the orders Hymenoptera (98.1%, Hemiptera (0.95%, Coleoptera (0.74% and Diptera (0.21% were collected. Most Hymenoptera individuals were bees of the genera Partamona and Trigona (677 individuals, which were considered of constant occurrence. Flowers producing up to 16.7 nectar mL were found. The nectar diet contained 16.44% of total sugar, and resulted in low survival of the bees in laboratory (31.32 . 2.37 hours, compared to a diet of 50% aqueous solution of honey (112.32 .2.03 hours.A flor do pau-de-balsa produz cerca de 10 a 15 mL de néctar, útil na atração de polinizadores, uma vez que o gênero Ochroma é incapaz de fazer autofecundação. É observada intensa mortalidade de abelhas em suas flores. Objetivou-se realizar o levantamento da frequência e constância de mortalidade de indivíduos da família Apidae, sendo os dados levantados no período de junho a agosto de 2008 em Lavras, MinasGerais, Brasil. Além disso, avaliou-se a sobrevivência de abelhas africanizadas alimentadas com o néctar desta flor quando comparados com aquelas alimentadas com solução aquosa de mel a 50%. Foram analisadas 40 flores e coletados 949 indivíduos das Ordens: Hymenoptera (98,1%, Hemiptera (0,95%, Coleoptera (0,74% e Diptera (0,21%. Dentre os himenópteros os mais frequentes foram dos g

  11. Nesting sites characteristics of stingless bees (Hymenoptera: Apidae in Central Sulawesi, Indonesia

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    Nelky Suriawanto

    2017-06-01

    Full Text Available Stingless bees (Hymenoptera: Apidae is eusocial insects that live together in a colony. This research was aimed to study the nesting site characteristics of stingless bees in the settlement areas at Central Sulawesi, Indonesia. The nesting sites were observed by purposive sampling method from July 2015 to January 2016. Four species belong to genus Tetragonula were found, namely T. fuscobalteata, T. biroi, T. sapiens, and T. laeviceps. Two spesies, T. biroi and T. sapiens are the new record in Sulawesi island. The highest abundance of stingless bees colony was T. fuscobalteata (92.26%, followed by T. biroi (4.17%, T. sapiens (2.98%, and T. laeviceps (0.59%. Nesting sites of T. fuscobalteata were found in the stone, brick wall, wooden wall, bamboo, and iron cavities, T. biroi in the wooden wall, stone, and brick wall cavities, T. sapiens in stone cavities, while T. laeviceps in wooden walls.

  12. Morphological, chemical and developmental aspects of the Dufour gland in some eusocial bees (Hymenoptera, Apidae: a review

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    Fábio Camargo Abdalla

    2006-06-01

    Full Text Available Morphological, chemical and developmental aspects of the Dufour gland in some eusocial bees (Hymenoptera, Apidae: a review. The present revision focused on the more recent data about the Dufour gland in some eusocial bees, taking in account general aspects of its morphology, secretion chemical nature, bio-synthetic pathway and development. Many functions have been attributed to this gland in eusocial bees, but none are convincing. With the new data about this gland, not only the secretion chemical pathway of the Dufour gland may be reasonable understood, as its function in some eusocial bees, especially Apis mellifera Linné, 1758, which has been extensively studied in the last years.Aspectos morfológicos, químicos e do desenvolvimento da glândula de Dufour em algumas abelhas eussociais (Hymenoptera, Apidae: revisão. Esta revisão aborda os mais recentes dados sobre a glândula de Dufour em algumas abelhas eussociais, considerando aspectos gerais da sua morfologia, do seu desenvolvimento, da natureza química da sua secreção, assim como sua via bio-sintética. Muitas funções têm-se atribuído à glândula de Dufour nas abelhas eussociais, mas nenhuma suficientemente convincente. Os novos dados a respeito dessa glândula permitem não só conhecer razoavelmente bem a via bio-sintética como a função da secreção da glândula de Dufour em algumas abelhas eussociais, especialmente em Apis mellifera Linné, 1758, a qual tem sido extensivamente estudada nos últimos anos.

  13. Adaptive behavior of Scotocryptus melitophilus Reitter (Coleoptera, Leiodidae to live with its host Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae

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    José Maurício Dias Bezerra

    2000-03-01

    Full Text Available Forty six beetles Scotocryptus melitophilus Reitter, 1881 collected in a nest of Melipona quadrifasciata Lepeletier, 1836 were studied. All beetles were put together in a large petri dish with 14 males, 14 virgin queens and 14 workers of M. quadrifasciata, which was not aggressive towards the S. melitophilus and flew with 3 to 10 beetles attached to them. When groups of 20 workers, 20 males and 20 virgin queens were put separately in three petri dishes, the workers become attractive to beetles after they were seven days old, while the males after the 19 days old. Only one virgin queen five days old attracted one beetle. The anatomic parts of the workers bees more attractive to the beetles when smeared to a filter paper were the head (32%, abdomen (29% and legs (29%. The part of the workers bees that less attracted the beetles was the thorax (10%. 5. melitophilus, both males and females, are able to distinguish caste, sex and age differences of their host M. quadrifasciata, what can increase the adaptive value of the beetles involved in phoretic behavior.

  14. Nesting biology of Centris (Hemisiella tarsata Smith (Hymenoptera, Apidae, Centridini

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    Cândida M. L. Aguiar

    2004-09-01

    Full Text Available Nests of Centris tarsata Smith, 1874 were obtained from trap-nests in areas of dry semi-deciduous forest (Baixa Grande and caatinga (Ipirá, in the State of Bahia. Nesting occurred in bamboo canes and in tubes of black cardboard with 5.8 cm (= small tube and 10.5 cm (= large tube in length and 0.6 and 0.8 cm in diameter, respectively. In both areas C. tarsata nested during the wet season producing four generations in Baixa Grande and three generations in Ipirá. The immatures of one generation underwent diapause at both sites. The bees constructed their nests with a mixture of sand and oil. In general, the cells were elongated and arranged in linear series with its opening pointing towards the nest entrance. Completed nests had two to three cells in small tubes, one to seven cells in large tubes, and two to 13 cells in bamboo canes. The nest plug resembled an uncompleted cell and was externally covered with oil. The cells were provisioned with pollen, oil, and nectar. Nests were parasitized by Mesocheira bicolor (Fabricius, 1804 (Hymenoptera: Apidae and other not identify bee species.Ninhos de Centris tarsata Smith, 1874 foram obtidos através da utilização de ninhos-armadilha, em áreas de floresta estacional semi-decídua (Baixa Grande e de caatinga (Ipirá, no Estado da Bahia. A nidificação ocorreu em gomos de bambus e em tubos de cartolina preta, estes com comprimentos de 5,8 cm (= tubos pequenos e 10,5 cm (= tubos grandes, e diâmetro de 0,6 e 0,8 cm, respectivamente. Em ambas as áreas C. tarsata nidificou durante a estação úmida, produzindo quatro gerações anuais em Baixa Grande e três em Ipirá. Os imaturos de uma das gerações passaram por diapausa em ambos os locais. As abelhas construíram seus ninhos com uma mistura de areia e óleo. Em geral, as células foram alongadas e arranjadas em série linear, com sua abertura dirigida para a entrada do ninho. Os ninhos completados tinham de duas a três células nos tubos pequenos

  15. Preservation of Domesticated Honey Bee (Hymenoptera: Apidae) Drone Semen.

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    Paillard, M; Rousseau, A; Giovenazzo, P; Bailey, J L

    2017-08-01

    Preservation of honey bee (Apis mellifera L., Hymenoptera: Apidae) sperm, coupled with instrumental insemination, is an effective strategy to protect the species and their genetic diversity. Our overall objective is to develop a method of drone semen preservation; therefore, two experiments were conducted. Hypothesis 1 was that cryopreservation (-196 °C) of drone semen is more effective for long-term storage than at 16 °C. Our results show that after 1 yr of storage, frozen sperm viability was higher than at 16 °C, showing that cryopreservation is necessary to conserve semen. However, the cryoprotectant used for drone sperm freezing, dimethyl sulfoxide (DMSO), can harm the queen and reduce fertility after instrumental insemination. Hypothesis 2 was that centrifugation of cryopreserved semen to reduce DMSO prior to insemination optimize sperm quality. Our results indicate that centrifuging cryopreserved sperm to remove cryoprotectant does not affect queen survival, spermathecal sperm count, or sperm viability. Although these data do not indicate that centrifugation of frozen-thawed sperm improves queen health and fertility after instrumental insemination, we demonstrate that cryopreservation is achievable, and it is better for long-term sperm storage than above-freezing temperatures for duration of close to a year. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  16. Onde os mais Adaptados Permanecem: Comunidade de Abelhas sem Ferrão (Hymenoptera: Apidae, Meliponini em Áreas Urbanas do Município de Ubá, Minas Gerais, Brasil

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    Gustavo Araújo

    2016-12-01

    Where the most Adapted Remain: Stingless Bees Community (Hymenoptera: Apidae, Meliponini in Urban Areas of the City of Uba, Minas Gerais, Brazil Abstract. The urbanization has caused the fragmentation of various natural environments, taking to loss of many ecological processes in which includes pollination, realized mainly by bees, in special Meliponini species, which has drastically reduced their populations due to loss of habitat for urban expansion. The aim of this study was to survey the species of stingless bees in urban areas of the municipality of Ubá - MG, Brazil, relate the richness and abundance of species found with the percentage of occupation of the structural variables of the urban landscape and relate the presence of the species obtained with these variables. Were found 28 nests belonging to four species, Tetragonisca angustula (Latreille, Trigona spinipes (Fabricius, Nannotrigona testaceicornis (Lepeletier and Friesella schrottkyi (Friese. There was no significant relationship between richness and abundance of Meliponini and structural variables of vegetation, possibly due to low values in the number of nests and species obtained. Only T. spinipes showed related to the presence of vegetation. The results show that the Meliponini community is composed by generalist species able to use artificial cavities and ornamental plants as an alternative source of resources, common in urban areas. The results obtained in this study is a warning to current conditions of the plant remaining in urban areas in the city of Ubá, besides may also be used as support in taking-decision on projects for the conservation and recovery of degraded areas, focusing on bees and their importance to the sustainability of these ecosystems.

  17. Mitochondrial sequencing reveals five separate origins of 'black' Apis mellifera (Hymenoptera: Apidae) in eastern Australian commercial colonies.

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    Oxley, P R; Oldroyd, B P

    2009-04-01

    Establishment of a closed population honey bee, Apis mellifera L. (Hymenoptera: Apidae), breeding program based on 'black' strains has been proposed for eastern Australia. Long-term success of such a program requires a high level of genetic variance. To determine the likely extent of genetic variation available, 50 colonies from 11 different commercial apiaries were sequenced in the mitochondrial cytochrome oxidase I and II intergenic region. Five distinct and novel mitotypes were identified. No colonies were found with the A. mellifera mellifera mitotype, which is often associated with undesirable feral strains. One group of mitotypes was consistent with a caucasica origin, two with carnica, and two with ligustica. The results suggest that there is sufficient genetic diversity to support a breeding program provided all these five sources were pooled.

  18. Potential pollinators of tomato, Lycopersicon esculentum (Solanaceae), in open crops and the effect of a solitary bee in fruit set and quality.

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    Santos, A O R; Bartelli, B F; Nogueira-Ferreira, F H

    2014-06-01

    We identified native bees that are floral visitors and potential pollinators of tomato in Cerrado areas, described the foraging behavior of these species, and verified the influence of the visitation of a solitary bee on the quantity and quality of fruits. Three areas of tomato crops, located in Minas Gerais, Brazil, were sampled between March and November 2012. We collected 185 bees belonging to 13 species. Exomalopsis (Exomalopsis) analis Spinola, 1853 (Hymenoptera: Apidae) was the most abundant. Ten species performed buzz pollination. Apis mellifera L. 1758 (Hymenoptera: Apidae) and Paratrigona lineata (Lepeletier, 1836) (Hymenoptera: Apidae) could also act as pollinators. The fruit set and number of seeds obtained from the pollination treatment by E. analis were higher than those in the control group. Our results allowed the identification of potential tomato pollinators in Cerrado areas and also contributed information regarding the impact of a single species (E. analis) on fruit set and quality. Although most of the visiting bees show the ability for tomato pollination, there is an absence of adequate management techniques, and its usage is difficult with the aim of increasing the crop production, which is the case for E. analis. Species such as Melipona quinquefasciata, P. lineata, and A. mellifera, which are easy to handle, are not used for pollination services. Finally, it is suggested that a combination of different bee species that are able to pollinate the tomato is necessary to prevent the super-exploitation of only a single species for pollination services and to guarantee the occurrence of potential pollinators in the crop area.

  19. Survival of honey bee (Hymenoptera: Apidae) spermatozoa incubated at room temperature from drones exposed to miticides.

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    Burley, Lisa M; Fell, Richard D; Saacke, Richard G

    2008-08-01

    We conducted research to examine the potential impacts ofcoumaphos, fluvalinate, and Apilife VAR (Thymol) on drone honey bee, Apis mellifera L. (Hymenoptera: Apidae), sperm viability over time. Drones were reared in colonies that had been treated with each miticide by using the dose recommended on the label. Drones from each miticide treatment were collected, and semen samples were pooled. The pooled samples from each treatment were subdivided and analyzed for periods of up to 6 wk. Random samples were taken from each treatment (n = 6 pools) over the 6-wk period. Sperm viability was measured using dual-fluorescent staining techniques. The exposure of drones to coumaphos during development and sexual maturation significantly reduced sperm viability for all 6 wk. Sperm viability significantly decreased from the initial sample to week 1 in control colonies, and a significant decrease in sperm viability was observed from week 5 to week 6 in all treatments and control. The potential impacts of these results on queen performance and failure are discussed.

  20. Meliponini neotropicais: o gênero Partamona Schwarz, 1939 (Hymenoptera, Apidae

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    Pedro Silvia R. M.

    2003-01-01

    Full Text Available Neotropical Meliponini: the genus Partamona Schwarz, 1939 (Hymenoptera, Apidae. The systematics and biogeography of Partamona Schwarz, a Neotropical genus of stingless bees (Meliponini, Apinae, Apidae, are revised. Seventeen new species are described: P. epiphytophila sp. nov., P. subtilis sp. nov., P. nhambiquara sp. nov., P. batesi sp. nov., P. yungarum sp. nov., P. vitae sp. nov., P. ferreirai sp. nov., P. gregaria sp. nov., P. auripennis sp. nov., P. nigrilabris sp. nov., P. combinata sp. nov., P. chapadicola sp. nov., P. seridoensis sp. nov., P. littoralis sp. nov., P. criptica sp. nov., P. rustica sp. nov. and P. sooretamae sp. nov. Partamona pseudomusarum Camargo, 1980, is considered as junior synonym of P. vicina Camargo, 1980. Types of P. grandipennis (Schwarz, 1951, P. xanthogastra Pedro & Camargo, 1996-1997, P. pearsoni (Schwarz, 1938, P. ailyae Camargo, 1980, P. pseudomusarum, P. vicina, P. mulata Moure in Camargo, 1980, P. aequatoriana Camargo, 1980, P. mourei Camargo, 1980, P. peckolti, (Friese, 1901, P. testacea (Klug, 1807, P. helleri (Friese, 1900 and P. musarum (Cockerell, 1917 were examined. Lectotypes of P. orizabaensis (Strand, 1919, and P. cupira (Smith, 1863 are designated. An identification key for the species and drawings of morphological characters are presented. A phylogenetic hypothesis, based mainly on morphological characters is proposed. Four groups are defined, considering the shape of mandible of workers and sternum VII of males: bilineata / epiphytophila group (western Amazon to México, including P. bilineata (Say, P. grandipennis, P. xanthogastra P. orizabaensis P. peckolti P. epiphytophila sp. nov., P. subtilis sp. nov., P. nhambiquara sp. nov., P. batesi sp. nov., P. yungarum sp. nov. and P. vitae sp. nov.; musarum group (Central Brazil, north of South America to Central America, including P. musarum, P. aequatoriana, P. vicina, P. mourei, P. pearsoni, P. ferreirai sp. nov., P. gregaria sp. nov. and P

  1. Optimizing Drone Fertility With Spring Nutritional Supplements to Honey Bee (Hymenoptera: Apidae) Colonies.

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    Rousseau, Andrée; Giovenazzo, Pierre

    2016-03-27

    Supplemental feeding of honey bee (Apis melliferaL., Hymenoptera: Apidae) colonies in spring is essential for colony buildup in northern apicultural regions. The impact of pollen and syrup feeding on drone production and sperm quality is not well-documented, but may improve fecundation of early-bred queens. We measured the impact of feeding sucrose syrup, and protein supplements to colonies in early spring in eastern Canada. Drones were reared under different nutritional regimes, and mature individuals were then assessed in regard to size, weight, and semen quality (semen volume, sperm count, and viability). Results showed significant increases in drone weight and abdomen size when colonies were fed sucrose and a protein supplement. Colonies receiving no additional nourishment had significantly less semen volume per drone and lower sperm viability. Our study demonstrates that feeding honey bee colonies in spring with sucrose syrup and a protein supplement is important to enhance drone reproductive quality. RÉSUMÉ: L'administration de suppléments alimentaires aux colonies de l'abeille domestique (Apis melliferaL., Hymenoptera: Apidae) au printemps est essentielle pour le bon développement des colonies dans les régions apicoles nordiques. L'impact de la supplémentation des colonies en pollen et en sirop sur la production des faux-bourdons et la qualité du sperme demeure peu documenté mais pourrait résulter en une meilleure fécondation des reines produites tôt en saison. Nous avons mesuré l'impact de la supplémentation en sirop et/ou en supplément de pollen sur les colonies d'abeilles tôt au printemps dans l'est du Canada. Les faux-bourdons ont été élevé sous différents régimes alimentaires et les individus matures ont ensuite été évalués pour leur taille, leur poids ainsi que la qualité de leur sperme (volume de sperme, nombre et viabilité des spermatozoïdes. Les résultats montrent une augmentation significative du poids et de la taille

  2. Prediction of social structure and genetic relatedness in colonies of the facultative polygynous stingless bee Melipona bicolor (Hymenoptera, Apidae).

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    Dos Reis, Evelyze Pinheiro; de Oliveira Campos, Lucio Antonio; Tavares, Mara Garcia

    2011-04-01

    Stingless bee colonies typically consist of one single-mated mother queen and her worker offspring. The stingless bee Melipona bicolor (Hymenoptera: Apidae) shows facultative polygyny, which makes this species particularly suitable for testing theoretical expectations concerning social behavior. In this study, we investigated the social structure and genetic relatedness among workers from eight natural and six manipulated colonies of M. bicolor over a period of one year. The populations of M. bicolor contained monogynous and polygynous colonies. The estimated genetic relatedness among workers from monogynous and polygynous colonies was 0.75 ± 0.12 and 0.53 ± 0.16 (mean ± SEM), respectively. Although the parental genotypes had significant effects on genetic relatedness in monogynous and polygynous colonies, polygyny markedly decreased the relatedness among nestmate workers. Our findings also demonstrate that polygyny in M. bicolor may arise from the adoption of related or unrelated queens.

  3. Prediction of social structure and genetic relatedness in colonies of the facultative polygynous stingless bee Melipona bicolor (Hymenoptera, Apidae

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    Evelyze Pinheiro dos Reis

    2011-01-01

    Full Text Available Stingless bee colonies typically consist of one single-mated mother queen and her worker offspring. The stingless bee Melipona bicolor (Hymenoptera: Apidae shows facultative polygyny, which makes this species particularly suitable for testing theoretical expectations concerning social behavior. In this study, we investigated the social structure and genetic relatedness among workers from eight natural and six manipulated colonies of M. bicolor over a period of one year. The populations of M. bicolor contained monogynous and polygynous colonies. The estimated genetic relatedness among workers from monogynous and polygynous colonies was 0.75 ± 0.12 and 0.53 ± 0.16 (mean ± SEM, respectively. Although the parental genotypes had significant effects on genetic relatedness in monogynous and polygynous colonies, polygyny markedly decreased the relatedness among nestmate workers. Our findings also demonstrate that polygyny in M. bicolor may arise from the adoption of related or unrelated queens.

  4. Mitochondrial DNA polymorphism among populations of Melipona quadrifasciata quadrifasciata Lepeletier (Apidae: Meliponini) from southern Brazil.

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    Torres, Rogelio R; Arias, Maria C; Moretto, Geraldo

    2009-01-01

    The geographical distribution of the Brazilian endemic stingless bee Melipona quadrifasciata quadrifasciata Lepeletier ranges from Rio Grande do Sul to Minas Gerais states. The objective of the present study was to verify mtDNA polymorphisms among samples of M. q. quadrifasciata collected in southern Brazil. Twenty nine colonies from three localities (Blumenau and Mafra/SC and Prudentópolis/ PR) were sampled. Seven mtDNA regions were amplified and further digested with 15 restriction enzymes (PCR-RFLP). Five composite haplotypes were identified, with two unique to samples from Prudentópolis and the remaining three to samples from Mafra and/or Blumenau.

  5. Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations.

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    Tavares, Mara Garcia; Pietrani, Nathalia Teixeira; de Castro Durvale, Maxwell; Resende, Helder Canto; de Oliveira Campos, Lucio Antonio

    2013-03-01

    Melipona quadrifasciata is a stingless bee widely found throughout the Brazilian territory, with two recognized subspecies, M. quadrifasciata anthidioides, that exhibits interrupted metasomal stripes, and M. quadrifasciata quadrifasciata, with continuous metasomal stripes. This study aimed to estimate the genetic variability of these subspecies. For this purpose, 127 colonies from 15 Brazilian localities were analyzed, using nine species-specific microsatellite primers. At these loci, the number of alleles ranged from three to 15 (mean: 7.2), and the observed heterozygosity (Ho) ranged from 0.03-0.21, while the expected heterozygosity (He) ranged from 0.23-0.47. The genetic distances among populations ranged from 0.03-0.45. The FST multilocus value (0.23) indicated that the populations sampled were structured, and the clustering analysis showed the formation of two subgroups and two more distant populations. The first group contained the subspecies M. quadrifasciata quadrifasciata, and the other, the subspecies M. quadrifasciata anthidioides and the two M. quadrifasciata populations with continuous metasomal stripes from northern Minas Gerais. These results confirmed that the yellow metasomal stripes alone are not a good means for correctly identifying the different subspecies of M. quadrifasciata.

  6. Systematics and biology of Xylocopa subgenus Schonnherria (Hymenoptera, Apidae) in Argentina.

    Science.gov (United States)

    Lucia, Mariano; Gonzalez, Victor H; Abrahamovich, Alberto H

    2015-01-01

    Biological information on the species of the large carpenter bee Xylocopa subgenus Schonnherria occurring in Argentina is revised. Based on the appraisal of museum specimens, the study of type material, and field surveys conducted across 15 provinces between 2007 and 2011, the following seven species are recognized for the country: Xylocopa bambusae Schrottky, Xylocopa chrysopoda Schrottky, Xylocopa macrops Lepeletier de Saint Fargeau, Xylocopa simillima Smith Xylocopa splendidula Lepeletier de Saint Fargeau, Xylocopa pulchra Smith, and Xylocopa viridis Smith. Previous literature records of Xylocopa dimidiata Latreille, Xylocopa subcyanea Pérez, and Xylocopa varians Smith for the province of Misiones appear to have been misidentified specimens, although the presence of these species in Argentina cannot be entirely ruled out given the proximity of this province to Brazil and Paraguay where they occur; Xylocopa boops Maidl was described from a male specimen with unusually enlarged eyes and is newly synonymized under Xylocopa macrops. Males and females of all species are diagnosed, described, and figured, including details of the male genitalia. Taxonomic comments, data on the geographical distribution and nesting substrates, and identification keys to all Argentinean species of Schonnherria are provided. The nesting biologies of Xylocopa splendidula and Xylocopa viridis are documented.

  7. Inventário da fauna de Euglossinae (Hymenoptera, Apidae do baixo sul da Bahia, Brasil Inventory of the Euglossinae (Hymenoptera, Apidae fauna of southern Bahia, Brazil

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    Edinaldo Luz das Neves

    1997-12-01

    Full Text Available This paper reports the results concerning collection of Euglossinae bees in a mangrove ecosystem in Valença(13º22'08"S and 39º04'20"W, Bahia. These samples were made twice a month, for a year. The chemical baits Citronella, Eucalyptol, Eugenol, Metyl Salicylate and Vanillin attracted 1,144 specimens distributed among twelve species and two genera: Eulaema (Lepeletier, 1841 and Euglossa (Latreille, 1802. The predominam bee species was Eulaema nigrita (Lepeletier, 1841 consisting of 49.4% of the collected specimens, followed by Euglossa cordata (Linnaeus, 1758 with 44.88%, Euglossa imperialis (Cockerell, 1922 with 2.4% and Eulaema meriana flavescens (Friese, 1899 with 1.6%. Theothers species, considering sporadic visitors, Euglossa securigera (Dressler, 1982, Euglossa chalybeata (Friese, 1925, Euglossa liopoda (Dressler, 1982, Euglossa gaianii (Dressler, 1982, Euglossa townsendi (Cockerell, 1904, Euglossa truncata (Rebelo & Moure, 1995, Euglossa melanotricha (Moure, 1967 and Euglossa sapphirina (Moure, 1968 represented together only 1.8% of the total sample. The Euglossinae were more active from October to May. Eucalyptol was the most attractive bait, attracting 1,120 specimens. Methyl Salicylate attracted 17 specimens, followed by Eugenol, which attracted 04 specimens and Vanillin, which attracted 03 specimens. Citronella was not an attractive chemical.

  8. Hymenoptera Stings and the Acute Kidney Injury

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    Yashad Dongol

    2013-07-01

    Full Text Available Hymenoptera stings are a health concern. Apidae (bees, Vespidae (hornets, yellow jackets and wasps and Formicidae (ants are medically-important stinging insects under the order Hymenoptera. Clinical features from simple skin manifestations to severe and fatal organ injury are due to the hypersensitivity reactions and/ or the toxic effects of the venom inoculated. Here we discuss on Hymenoptera stings involving apids (honey bees and vespids (wasps, hornets and yellow jackets and their effect on renal function and associated morphological changes in the kidney. Despite the differences in venom composition and quantity released per sting in two insect groups, both lead to similar medical consequences, such as localised normal allergic reactions, mild to severe anaphylaxis and shock and multiple organ and tissue injury leading to multiple organ failure. Acute kidney injury (AKI is one of the unusual complications of Hymenoptera stings and has the basis of both immune-mediated and toxic effects. Evidence has proven that supportive therapy along with the standard medication is very efficient in completely restoring the kidney function without any recurrence.

  9. Dinâmica de populações de Euglossina (Hymenoptera, Apidae em mata ciliar, Urbano Santos, Maranhão, Brasil Population dynamics of Euglossina (Hymenoptera, Apidae in riparian forest, Urbano Santos, Maranhão, Brazil

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    Cristiane C. de Carvalho

    2006-06-01

    Full Text Available Machos de Euglossina foram coletados por meio de iscas-odores de benzoato de benzila, eucaliptol, eugenol, salicilato de metila, vanilina, durante um ano em área de mata ciliar, no município de Urbano Santos, Maranhão. As coletas foram realizadas mensalmente, entre 8 h e 16 h, totalizando 96 horas de amostragem. Foram amostrados 283 indivíduos, 4 gêneros e 16 espécies. Euglossa Latreille, 1802 foi o gênero mais abundante, seguido por Eufriesea Cockerell, 1909, Eulaema Lepeletier, 1841 e Exaerete Hoffmannsegg, 1817. As espécies mais freqüentes foram Euglossa modestior (Dressler, 1982, Euglossa cordata (Linnaeus, 1758, Eulaema cingulata (Fabricius, 1804, Exaerete smaragdina (Guérin-Menéville, 1845, Eulaema nigrita Lepeletier, 1841 e Euglossa gaianii Dressler, 1982. Eucaliptol foi a essência mais atrativa. As maiores freqüências de visitas ocorreram no período da manhã e a maior diversidade de espécies ocorreu no período chuvoso.Males of Euglossina bees were collected in benzil benzoate, eucaliptol, eugenol, methyl salicylate and vanillin scent baits, during one year in a riparian forest area, located in the municipality of Urbano Santos, Maranhão. The collections were carried out monthly, between 8 am and 4 pm, totalling 96 hours of sampling, resulting in 283 individuals, 4 genera and 16 species. Euglossa Latreille, 1802 was the most abundant genus, followed by Eufriesea Cockerell, 1909, Eulaema Lepeletier, 1841 and Exaerete Hoffmannsegg, 1817. The most frequent species were Euglossa modestior (Dressler, 1982, Euglossa cordata (Linnaeus, 1758, Eulaema cingulata (Fabricius, 1804, Exaerete smaragdina (Guérin-Menéville, 1845, Eulaema nigrita Lepeletier, 1841 and Euglossa gaianii (Dressler, 1982. Eucaliptol was the most attractive chemical bait. The highest frequencies of visits were in the morning and the highest diversity of species occurred in the rainy period.

  10. Nest-mate recognition in Manuelia postica (Apidae: Xylocopinae): an eusocial trait is present in a solitary bee.

    Science.gov (United States)

    Flores-Prado, Luis; Aguilera-Olivares, Daniel; Niemeyer, Hermann M

    2008-02-07

    In eusocial Hymenoptera, females are more tolerant towards nest-mate than towards non-nest-mate females. In solitary Hymenoptera, females are generally aggressive towards any conspecific female. Field observations of the nest biology of Manuelia postica suggested nest-mate recognition. Experiments were performed involving two live interacting females or one live female interacting with a dead female. Live females from different nests were more intolerant to each other than females from the same nest. Females were more intolerant towards non-nest-mate than towards nest-mate dead females. When dead females were washed with pentane, no differences in tolerant and intolerant behaviours were detected between non-nest-mate and nest-mate females. Females were more intolerant towards nest-mate female carcasses coated with the cuticular extract from a non-nest-mate than towards non-nest-mate female carcasses coated with the cuticular extract from a nest-mate. The compositions of the cuticular extracts was more similar between females from the same nest than between females from different nests. The results demonstrate for the first time nest-mate recognition mediated by cuticular chemicals in a largely solitary species of Apidae. The position of Manuelia at the base of the Apidae phylogeny suggests that nest-mate recognition in eusocial species apical to Manuelia represents the retention of a primitive capacity in Apidae.

  11. The Size But not the Symmetry of the Wings of Eulaema nigrita Lepeletier (Apidae: Euglossini) is Affected by Human-Disturbed Landscapes in the Brazilian Cerrado Savanna.

    Science.gov (United States)

    Pinto, N S; Silva, D P; Rodrigues, J G; De Marco, P

    2015-10-01

    Among other human-related activities, habitat loss and fragmentation are currently ranked as the most important environmental features affecting the persistence of animal and plant populations in landscapes, as well as the maintenance of ecological processes and services. Since these processes are also capable of affecting the ontogenetic development of species inhabiting those landscapes, here we measured the wing veins of male Eulaema nigrita Lepeletier (Apidae: Euglossini) bees in order to evaluate whether the bees sampled in agriculture (AG) areas suffer higher fluctuating asymmetry (FA) than those sampled in Cerrado (CE) areas in the Brazilian state of Goiás. We believe that individuals sampled in CE areas would be less asymmetric than those sampled in AG areas, given a potential higher exposure of these specimens to environmental stresses (mostly pesticides). However, we did not observe a significant trend in the FA measures we obtained, although three wing measures were bigger for bees from CE areas. The lack of significant effects of FA may be related to inherent bionomic features of E. nigrita. For instance, given their high individual dispersal abilities, the individuals we analyzed may have developed in different areas than those where they were sampled. Their generalist feeding behavior may also have given them a higher resistance to environmental perturbations, buffering the normal development of immatures even in areas with local high environmental stress. Nonetheless, higher death rates of individuals from anthropic areas may also have killed the developing immatures of E. nigrita before they reached adulthood consequently equalizing our sampled estimates.

  12. Study of the flight range and ideal density of the africanized honeybees, Apis mellifera L., 1758 (Hymenoptera: Apidae) labelled with {sup 32} P on an apple orchard; Estudo do raio de acao e densidade ideal de abelhas africanizadas, Apis mellifera L., 1758 (Hymenoptera: Apidae) marcadas com {sup 32} P em pomar de maca

    Energy Technology Data Exchange (ETDEWEB)

    Paranhoa, B A.J.

    1990-06-01

    The ideal density, the flight range, the choice for any flight direction, the influence of temperature and relative humidity of air about the honeybee`s activity, Apis mellifera L.. 1758 (Hymenoptera: Apidae) were studied in an apple orchard, utilizing nuclear techniques. Five hives, with 35,000 bees each, were labelled with syrup (50%) content (2,5 {mu}Ci {sup 32} P/ml) and taken one by one, every two days to the blossomed orchard. A circumference area of 100 m diameter (0,8 ha) W staked each 10 m from the center to the limit (50 m), making a cross, pointing out to North, South, East and West. The honeybees were collected on apple flowers, during 5 minutes in each stake, at 10:00 a.m. and 12:30 p.m. (author).

  13. Ocorrência do pólen de Podocarpus sp. (Podocarpaceae nas coletas de Frieseomelitta varia Lepeletier 1836 (Apidae: Meliponinae em uma área de Manaus, AM, Brasil Occurrence of Podocarpus sp. (Podocarpaceae pollen on Frieseomelitta varia Lepeletier 1836 (Apidae: Meliponinae in Manaus, Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Antonio Carlos Marques-Souza

    2010-06-01

    Full Text Available Durante o período de doze meses, o pólen transportado por Frieseomelitta varia Lepeletier 1836 foi coletado das corbículas das operárias, logo após o fechamento da entrada das colméias. Feita a identificação polínica dos grãos de pólen e sua frequência mensal nas amostras, constatou-se que as espécies das famílias mais visitadas pela ordem de coleta, foram: Cecropiaceae, Sapotaceae, Myrtaceae e Moraceae. As operárias coletaram o pólen de 79 espécies de plantas distribuídas em 60 gêneros e 37 famílias, sendo as mais frequentes: Cecropia sp. visitada o ano todo com atratividade em janeiro de 82,03%, Morus sp. com atratividade de 37,46% em dezembro; Myrcia amazonica DC. com atratividade em abril de 32,34% e Pouteria macrophylla (A.DC. Eyma em junho com atratividade de 36,54%. Quanto a Podocarpus sp. esse é o primeiro relato da presença do pólen dessa espécie em coletas de meliponíneos, o que não deixa de ser um fato curioso, uma vez que se trata de uma gimnosperma encontrada em áreas específicas da região amazônica e que apresentou atratividade para Frieseomelitta varia Lepeletier 1836 de 4,94% no mês de março.Over a 12 month period, pollen was sampled from the corbiculae of workers of the stingless bee Frieseomelitta varia Lepeletier 1836. Collections were obtained immediately after sealing the hives, located in a forest fragment in the city of Manaus. Pollen was identified and monthly frequency of each type was tallied. The most-visited plant families in decreasing order of frequency were Cecropiaceae, Sapotaceae, Myrtaceae and Moraceae. Identified pollen was harvested from 79 plant species in 60 genera and 37 families. The most frequent species were: Cecropia sp., visited year-round but with highest attractiveness (82% in January; Morus sp., with peak attractiveness (37% in December; Myrcia amazonica DC. with 32% peak attractiveness in April; and Pouteria macrophylla (A.DC. Eyma, with 37% peak attractiveness in

  14. Occurrence, morphology and ultrastructure of the Dufour gland in Melipona bicolor Lepeletier (Hymenoptera, Meliponini Ocorrência, morfologia e ultra-estrutura da glândula de Dufour em Melipona bicolor Lepeletier (Hymenoptera, Meliponini

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    Fábio Camargo Abdalla

    2004-03-01

    Full Text Available The occurrence, morphology and ultrastructure of the Dufour gland in Melipona bicolor Lepeletier, 1836 are presented. The Dufour gland is not present in workers. In virgin queens the gland cells show characteristics of low activity, which are described in the text. In physogastric queens the gland epithelium is higher and the cells more active than in virgin queens, showing numerous basal plasmic membrane invaginations impregnated by an electrondense material, increased apical invaginations and accumulation of substances that will be released to the gland lumen in the subcuticular space. Therefore, the data show that the Dufour gland is more developed in physogastric than in virgin queens, indicating a possible involvement of the Dufour gland in the reproduction of this species.A ocorrência, morfologia e ultra-estrutura da glândula de Dufour em Melipona bicolor Lepeletier, 1836 são apresentados. A glândula de Dufour não está presente nas operárias. Nas rainhas virgens, as células glandulares mostram características de baixa atividade, as quais são descritas no texto. Nas rainhas fisogástricas, o epitélio glandular é mais alto e as células mais ativas do que nas rainhas virgens, mostrando numerosas invaginações da membrana plasmática basal impregnadas por material eletrondenso, além do aumento da freqüência de invaginações apicais e acúmulo de substâncias no espaço subcuticular, as quais serão posteriormente liberadas ao lúmen da glândula. Portanto, os dados mostram que a glândula de Dufour é mais desenvolvida nas rainhas fisogástricas do que nas virgens, indicando um possível envolvimento da glândula de Dufour na reprodução desta espécie.

  15. Potential of ozone as a fumigant to control pests in honey bee (Hymenoptera: Apidae) hives.

    Science.gov (United States)

    James, R R

    2011-04-01

    Ozone is a powerful oxidant capable of killing insects and microorganisms, and eliminating odors, taste, and color. Thus, it could be useful as a fumigant to decontaminate honey comb between uses. The experiments here are intended to determine the exposure levels required to kill an insect pest and spore forming bee pathogens. Ozone was effective against greater wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae), even on naturally infested comb. Neonates and adults were the easiest life stages to kill, requiring only a few hours of exposure, whereas eggs required a 48-h exposure (at 460-920 mg O3/m3). Two honey bee, Apis mellifera L. (Hymenoptera: Apidae), pathogens, Ascosphaera apis (a fungus that causes chalkbrood) and Paenibacillus larvae (a bacterium that causes American foulbrood), also were killed with ozone. These pathogens required much higher concentrations (3200 and 8560 mg O3/m3, respectively) and longer exposure periods (3 d) than needed to control the insects. P. larvae was effectively sterilized only when these conditions were combined with high temperature (50 degrees C) and humidity (> or =75% RH). Thus, ozone shows potential as a fumigant for bee nesting materials, but further research is needed to evaluate its acceptability and efficacy in the field. The need for a reliable method to decontaminate honey bee nesting materials as part of an overall bee health management system is discussed.

  16. Effect of entomopathogens on Africanized Apis mellifera L. (Hymenoptera: Apidae

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    Michele Potrich

    2018-01-01

    Full Text Available This study aimed to evaluate the effect of commercially used entomopathogens on Africanized Apis mellifera L. (Hymenoptera: Apidae. Four bioassays were performed: 1 pulverized entomopathogens on A. mellifera; 2 entomopathogens sprayed on a smooth surface; 3 entomopathogens sprayed on soy leaves; and 4 entomopathogens mixed with candy paste (sugar syrup. Five treatments were prepared: sterile distilled water (control, distilled water sterilized with Tween® 80 (0.01%, and the commercial entomopathogens Metarhizium anisopliae E9 (1.0 × 109 conidia mL−1, Beauveria bassiana PL63 (1.0 × 108 conidia mL−1 and Bacillus thuringiensis var. kurstaki HD-1 (3.0 × 108 spores mL−1. Each treatment consisted of five repetitions, with 20 workers per repetition, which were stored in a plastic box and, later, in a biological oxygen demand (B.O.D. incubator (27 ± 2 °C, RH of 60% ± 10%, 12-h photophase. The mortality of the workers was evaluated from 1 h to 240 h, and the data were analyzed using Bayesian inference. The workers killed by the ingestion of candy paste contaminated with the pathogens (products were randomly separated and selected for the removal of the midgut. Each midgut was fixed in Bouin's solution and prepared for histology. B. bassiana was verified to reduce the survival of A. mellifera workers in all bioassays. Moreover, M. anisopliae reduced the survival of A. mellifera workers directly sprayed, on a smooth surface and mixed with candy. B. thuringiensis reduced A. mellifera survival on a smooth surface and mixed with candy paste. However, its effects were lower than that observed by B. bassiana. The treatments with the biological products did not induce morphometric alterations in the midgut of A. mellifera. Keywords: Bayesian statistics, Entomopathogenic fungi, Entomopathogenic bacteria, Honeybee, Selectivity

  17. Study of the flight range and ideal density of the africanized honeybees, Apis mellifera L., 1758 (Hymenoptera: Apidae) labelled with 32 P on an apple orchard

    International Nuclear Information System (INIS)

    Paranhoa, B.A.J.

    1990-06-01

    The ideal density, the flight range, the choice for any flight direction, the influence of temperature and relative humidity of air about the honeybee's activity, Apis mellifera L.. 1758 (Hymenoptera: Apidae) were studied in an apple orchard, utilizing nuclear techniques. Five hives, with 35,000 bees each, were labelled with syrup (50%) content (2,5 μCi 32 P/ml) and taken one by one, every two days to the blossomed orchard. A circumference area of 100 m diameter (0,8 ha) W staked each 10 m from the center to the limit (50 m), making a cross, pointing out to North, South, East and West. The honeybees were collected on apple flowers, during 5 minutes in each stake, at 10:00 a.m. and 12:30 p.m. (author)

  18. Varroa destructor (Mesostigmata: Varroidae in Costa Rica: population dynamics and its influence on the colony condition of Africanized honey bees (Hymenoptera: Apidae

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    Rafael A Calderón

    2008-12-01

    Full Text Available The development of Varroa destructor Anderson & Trueman (Mesostigmata: Varroidae population dynamics in Africanized honey bees, Apis mellifera L. (Hymenoptera: Apidae colonies was monitored from February to July 2004 in Atenas, Costa Rica. A correlation between the mite infestation level and the colony condition was evaluated. For each colony, infestation of varroa in adult bees was measured twice a month. Sticky boards were placed on the bottom boards of each colony to collect fallen mites. The condition of the colonies was evaluated by measuring the amount of brood and adult bees. Our results consistently showed that mite infestation on adult bees increased significantly in the experimental colonies, rising to 10.0% by the end of the experiment. In addition, the mean mite fall increased significantly over the course of the study in the treated (R= 0.72, PLa dinámica poblacional del ácaro Varroa destructor Anderson & Trueman (Mesostigmata: Varroidae en abejas africanizadas, Apis mellifera L. (Hymenoptera: Apidae fue monitoreada de febrero a julio 2004, en Atenas, Costa Rica. Asimismo, se analizó la relación entre el nivel de infestación de varroa y la condición de la colmena. La infestación del ácaro V. destructor fue evaluada en abejas adultas dos veces al mes. Además, se colocaron trampas adhesivas en el fondo de la colmena para recoger los ácaros que caen naturalmente. La condición de la colmena fue determinada midiendo la cantidad de cría y la población de abejas adultas. La infestación del ácaro V. destructor en abejas adultas aumentó significativamente durante el estudio hasta alcanzar un 10.0%. Igualmente, la caída natural de ácaros se incrementó, tanto en colmenas que fueron tratadas previa-mente con un acaricida químico (R= 0.72, P<0.05 como en colmenas sin tratamiento (R= 0.74, P<0.05, hasta llegar a 63.8 y 73.5 ácaros por día, respectivamente. El aumento de la infestación en las colmenas coincidió con una

  19. Phylogeny and revision of the bee genus Rhinocorynura Schrottky (Hymenoptera, Apidae, Augochlorini, with comments on its female cephalic polymorphism

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    Rodrigo B. Gonçalves

    2012-03-01

    Full Text Available Phylogeny and revision of the bee genus Rhinocorynura Schrottky (Hymenoptera, Apidae, Augochlorini, with comments on its female cephalic polymorphism. A taxonomic revision and a phylogeny for the species of Rhinocorynura are provided. Six species are recognized: R. briseis, R. crotonis, R. inflaticeps and R. vernoniae stat. nov., the latter removed from synonymy with R. inflaticeps, in addition to two newly described species, R. brunnea sp. nov. and R. viridis sp. nov. Lectotypes for Halictus crotonis Ducke, 1906 and Halictus inflaticeps Ducke, 1906 are hereby designated. Another available name included in Rhinocorynura, Corynuropsis ashmeadi Schrottky, 1909, is removed from the genus and treated as species inquerenda in Augochlorini. Rhinocorynura is monophyletic in the phylogenetic analysis and the following relationships were found among its species: (R. crotonis (R. briseis ((R. brunnea sp. nov. + R. viridis sp. nov. (R. inflaticeps + R. vernoniae. Biogeographic relationships within the genus and comparisons with related taxa are presented. Females of all species exhibit pronounced variation in body size, in two of them, R. inflaticeps and R. vernoniae, with structural modifications possibly linked to division of labor. Identification key and illustrations for the species are provided.

  20. A Landscape Analysis to Understand Orientation of Honey Bee (Hymenoptera: Apidae) Drones in Puerto Rico.

    Science.gov (United States)

    Galindo-Cardona, A; Monmany, A C; Diaz, G; Giray, T

    2015-08-01

    Honey bees [Apis mellifera L. (Apidae, Hymenoptera)] show spatial learning behavior or orientation, in which animals make use of structured home ranges for their daily activities. Worker (female) orientation has been studied more extensively than drone (male) orientation. Given the extensive and large flight range of drones as part of their reproductive biology, the study of drone orientation may provide new insight on landscape features important for orientation. We report the return rate and orientation of drones released at three distances (1, 2, and 4 km) and at the four cardinal points from an apiary located in Gurabo, Puerto Rico. We used high-resolution aerial photographs to describe landscape characteristics at the releasing sites and at the apiary. Analyses of variance were used to test significance among returning times from different distances and directions. A principal components analysis was used to describe the landscape at the releasing sites and generalized linear models were used to identify landscape characteristics that influenced the returning times of drones. Our results showed for the first time that drones are able to return from as far as 4 km from the colony. Distance to drone congregation area, orientation, and tree lines were the most important landscape characteristics influencing drone return rate. We discuss the role of landscape in drone orientation. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  1. Interações agressivas em abelhas sem ferrão: Melipona quadrifasciata (Lepeletier invadindo ninho de Scaptotrigona bipunctata (Lepeletier

    Directory of Open Access Journals (Sweden)

    2015-08-01

    Abstract. This record describes the occurrence of conflicts between stingless bees of an active colony of Scaptotrigona bipunctata (Lepeletier and individuals of Melipona quadrifasciata (Lepeletier, and discusses possible hypotheses that motivated the attack. Behaviors were observed in an active colony of S. bipunctata. The active nest guards detained individuals of M. quadrifasciata who invaded the colony. The chances of misidentification of the colony entrance and error in the species possible aggregation were discarded, however, the hypothesis of the real invasion recorded in this study demonstrates a behavioral strategy by M. quadrifasciata suggests that there is an energy savings if successful in looting.

  2. Phylogeny and revision of the bee genus Rhinocorynura Schrottky (Hymenoptera, Apidae, Augochlorini, with comments on its female cephalic polymorphism

    Directory of Open Access Journals (Sweden)

    Rodrigo B. Gonçalves

    2012-03-01

    Full Text Available Phylogeny and revision of the bee genus Rhinocorynura Schrottky (Hymenoptera, Apidae, Augochlorini, with comments on its female cephalic polymorphism. A taxonomic revision and a phylogeny for the species of Rhinocorynura are provided. Six species are recognized: R. briseis, R. crotonis, R. inflaticeps and R. vernoniae stat. nov., the latter removed from synonymy with R. inflaticeps, in addition to two newly described species, R. brunnea sp. nov. and R. viridis sp. nov. Lectotypes for Halictus crotonis Ducke, 1906 and Halictus inflaticeps Ducke, 1906 are hereby designated. Another available name included in Rhinocorynura, Corynuropsis ashmeadi Schrottky, 1909, is removed from the genus and treated as species inquerenda in Augochlorini. Rhinocorynura is monophyletic in the phylogenetic analysis and the following relationships were found among its species: (R. crotonis (R. briseis ((R. brunnea sp. nov. + R. viridis sp. nov. (R. inflaticeps + R. vernoniae. Biogeographic relationships within the genus and comparisons with related taxa are presented. Females of all species exhibit pronounced variation in body size, in two of them, R. inflaticeps and R. vernoniae, with structural modifications possibly linked to division of labor. Identification key and illustrations for the species are provided.Filogenia e revisão taxonômica das abelhas do gênero Rhinocorynura Schrottky (Hymenoptera, Apidae, Augochlorini, com comentários sobre o poliformismo cefálico das fêmeas. São apresentadas uma revisão taxonômica e filogenia para as espécies de Rhinocorynura. Seis espécies são reconhecidas, duas descritas como novas, R. brunnea sp. nov. e R. viridis sp. nov., e quatro com nomes disponíveis, R. briseis, R. crotonis, R. inflaticeps e R. vernoniae stat. nov., esta última removida da sinonímia com R. inflaticeps. Designam-se aqui lectótipos para Halictus crotonis Ducke, 1906 e Halictus inflaticeps Ducke, 1906. Outro nome disponível incluído em

  3. Sublethal Effects of the Neonicotinoid Insecticide Thiamethoxam on the Transcriptome of the Honey Bees (Hymenoptera: Apidae).

    Science.gov (United States)

    Shi, Teng-Fei; Wang, Yu-Fei; Liu, Fang; Qi, Lei; Yu, Lin-Sheng

    2017-12-05

    Neonicotinoid insecticides are now the most widely used insecticides in the world. Previous studies have indicated that sublethal doses of neonicotinoids impair learning, memory capacity, foraging, and immunocompetence in honey bees (Apis mellifera, Linnaeus) (Hymenoptera: Apidae). Despite these, few studies have been carried out on the molecular effects of neonicotinoids. In this study, we focus on the second-generation neonicotinoid thiamethoxam, which is currently widely used in agriculture to protect crops. Using high-throughput RNA-Seq, we investigated the transcriptome profile of honey bees after subchronic exposure to 10 ppb thiamethoxam over 10 d. In total, 609 differentially expressed genes (DEGs) were identified, of which 225 were upregulated and 384 were downregulated. Several genes, including vitellogenin, CSP3, defensin-1, Mrjp1, and Cyp6as5 were selected and further validated using real-time quantitative polymerase chain reaction assays. The functions of some DEGs were identified, and Gene Ontology-enrichment analysis showed that the enriched DEGs were mainly linked to metabolism, biosynthesis, and translation. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that thiamethoxam affected biological processes including ribosomes, the oxidative phosphorylation pathway, tyrosine metabolism pathway, pentose and glucuronate interconversions, and drug metabolism. Overall, our results provide a basis for understanding the molecular mechanisms of the complex interactions between neonicotinoid insecticides and honey bees. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  4. Larval development of Physocephala (Diptera, Conopidae in the bumble bee Bombus morio (Hymenoptera, Apidae

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    Fábio C Abdalla

    2014-12-01

    Full Text Available Larval development of Physocephala (Diptera, Conopidae in the bumble bee Bombus morio (Hymenoptera, Apidae. In the summer of 2012, a high incidence of conopid larvae was observed in a sample of female B. morio collected in remaining fragments of semidecidual forest and Cerrado, in the municipality of Sorocaba, state of São Paulo, Brazil. The larval development of conopid flies was studied, beginning at the larval instars (LO to L3 and PUP, until the emergence of the imago under laboratory conditions and inside the host. At the first instar, or LO, the microtype larvae measured less than 1 mm in length. During the transition from L1 to L3, the larvae grew in length. At L3, the larvae doubled their length (4 mm and then started to develop both in length and width, reaching the PUP stage with 10 mm in length and 7 mm in width. The main characteristic that differentiates L3 from the early instars is the larger body size and the beginning of posterior spiracle development. The development from PUP to puparium took less than 24h. The bees died ten days after the fly oviposition, or just before full PUP development. The early development stages (egg-LO to L1 were critical for larva survival. The pupa was visible between the intersegmental sternites and, 32 days after pupation, a female imago of Physocephala sp. emerged from one bee. The puparium and the fly measured approximately 10 mm in length. In a single day of collection, up to 45% of the bumble bees collected were parasitized by conopid flies.

  5. USBombus, a database of contemporary survey data for North American Bumble Bees (Hymenoptera, Apidae, Bombus) distributed in the United States.

    Science.gov (United States)

    Koch, Jonathan B; Lozier, Jeffrey; Strange, James P; Ikerd, Harold; Griswold, Terry; Cordes, Nils; Solter, Leellen; Stewart, Isaac; Cameron, Sydney A

    2015-01-01

    Bumble bees (Hymenoptera: Apidae, Bombus) are pollinators of wild and economically important flowering plants. However, at least four bumble bee species have declined significantly in population abundance and geographic range relative to historic estimates, and one species is possibly extinct. While a wealth of historic data is now available for many of the North American species found to be in decline in online databases, systematic survey data of stable species is still not publically available. The availability of contemporary survey data is critically important for the future monitoring of wild bumble bee populations. Without such data, the ability to ascertain the conservation status of bumble bees in the United States will remain challenging. This paper describes USBombus, a large database that represents the outcomes of one of the largest standardized surveys of bumble bee pollinators (Hymenoptera, Apidae, Bombus) globally. The motivation to collect live bumble bees across the United States was to examine the decline and conservation status of Bombus affinis, B. occidentalis, B. pensylvanicus, and B. terricola. Prior to our national survey of bumble bees in the United States from 2007 to 2010, there have only been regional accounts of bumble bee abundance and richness. In addition to surveying declining bumble bees, we also collected and documented a diversity of co-occuring bumble bees. However we have not yet completely reported their distribution and diversity onto a public online platform. Now, for the first time, we report the geographic distribution of bumble bees reported to be in decline (Cameron et al. 2011), as well as bumble bees that appeared to be stable on a large geographic scale in the United States (not in decline). In this database we report a total of 17,930 adult occurrence records across 397 locations and 39 species of Bombus detected in our national survey. We summarize their abundance and distribution across the United States and

  6. First host record for the cleptoparasitic bee Rhathymus friesei Ducke (Hymenoptera, Apidae

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    Hugo A. Werneck

    2012-12-01

    Full Text Available The genus Rhathymus contains only obligatory cleptoparasitic species whose hosts belong to the genus Epicharis (Apidae, Centridini. Host information is available for only four of the 20 species of Rhathymus. In this note a new host record is added, in which the parasitism by R. friesei on nests of Epicharis (Epicharoides picta is documented.

  7. Floral preferences of a neotropical stingless bee, Melipona quadrifasciata Lepeletier (Apidae: Meliponina) in an urban forest fragment.

    Science.gov (United States)

    Antonini, Y; Costa, R G; Martins, R P

    2006-05-01

    Species of plants used by Melipona quadrifasciata Lepeletier for pollen and nectar gathering in an urban forest fragment were recorded in Belo Horizonte, Minas Gerais, Brazil. Melipona quadrifasciata visited 22 out of 103 flowering plant species. The plant species belonged mainly to Myrtaceae, Asteraceae, and Convolvulaceae (64% of the visits). Melipona quadrifasciata tended to collect pollen or nectar each time, except for Myrtaceae species, from which both pollen and nectar were collected. Bee abundance at flowers did not significantly correlate to food availability (expressed by flowering plant richness). We found a relatively high similarity (50%) between plant species used by M. quadrifasciata, which was also found in studies carried out in São Paulo State. However, low similarity (17%) was found between the results of this study and those of another done in Bahia State, Brazil.

  8. Floral preferences of a neotropical stingless bee, Melipona quadrifasciata Lepeletier (Apidae: Meliponina in an urban forest fragment

    Directory of Open Access Journals (Sweden)

    Y. Antonini

    Full Text Available Species of plants used by Melipona quadrifasciata Lepeletier for pollen and nectar gathering in an urban forest fragment were recorded in Belo Horizonte, Minas Gerais, Brazil. Melipona quadrifasciata visited 22 out of 103 flowering plant species. The plant species belonged mainly to Myrtaceae, Asteraceae, and Convolvulaceae (64% of the visits. Melipona quadrifasciata tended to collect pollen or nectar each time, except for Myrtaceae species, from which both pollen and nectar were collected. Bee abundance at flowers did not significantly correlate to food availability (expressed by flowering plant richness. We found a relatively high similarity (50% between plant species used by M. quadrifasciata, which was also found in studies carried out in São Paulo State. However, low similarity (17% was found between the results of this study and those of another done in Bahia State, Brazil.

  9. Expression profile of a Laccase2 encoding gene during the metamorphic molt in Apis mellifera (Hymenoptera,Apidae

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    Moysés Elias-Neto

    2013-06-01

    Full Text Available Expression profile of a Laccase2 encoding gene during the metamorphic molt in Apis mellifera (Hymenoptera, Apidae. Metamorphosis in holometabolous insects occurs through two subsequent molting cycles: pupation (metamorphic molt and adult differentiation (imaginal molt. The imaginal molt in Apis mellifera L. was recently investigated in both histological and physiological-molecular approaches. Although the metamorphic molt in this model bee is extremely important to development, it is not well-known yet. In the current study we used this stage as an ontogenetic scenario to investigate the transcriptional profile of the gene Amlac2, which encodes a laccase with an essential role in cuticle differentiation. Amlac2 expression in epidermis was contrasted with the hemolymph titer of ecdysteroid hormones and with the most evident morphological events occurring during cuticle renewal. RT-PCR semiquantitative analyses using integument samples revealed increased levels of Amlac2 transcripts right after apolysis and during the subsequent pharate period, and declining levels near pupal ecdysis. Compared with the expression of a cuticle protein gene, AmelCPR14, these results highlighted the importance of the ecdysteroid-induced apolysis as an ontogenetic marker of gene reactivation in epidermis for cuticle renewal. The obtained results strengthen the comprehension of metamorphosis in Apis mellifera. In addition, we reviewed the literature about the development of A. mellifera, and emphasize the importance of revising the terminology used to describe honey bee molting cycles.

  10. Mitochondrial genome of the North African Sahara Honeybee, Apis mellifera sahariensis (Hymenoptera: Apidae)

    DEFF Research Database (Denmark)

    Haddad, Nizar; Adjlane, Noureddine; Loucif-Ayad, Wahida

    2017-01-01

    e present the complete mitochondrial genome of honey bee subspecies, Apis mellifera sahariensis (Apidae) belonging to the African lineage. The assembled circular genome has a length of 16,569 bp which comprises 13 protein coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and AT rich...

  11. Brood pheromone effects on colony protein supplement consumption and growth in the honey bee (Hymenoptera: Apidae) in a subtropical winter climate.

    Science.gov (United States)

    Pankiw, Tanya; Sagili, Ramesh R; Metz, Bradley N

    2008-12-01

    Fatty acid esters extractable from the surface of honey bee, Apis mellifera L. (Hymenoptera: Apidae), larvae, called brood pheromone, significantly increase rate of colony growth in the spring and summer when flowering plant pollen is available in the foraging environment. Increased colony growth rate occurs as a consequence of increased pollen intake through mechanisms such as increasing number of pollen foragers and pollen load weights returned. Here, we tested the hypothesis that addition of brood pheromone during the winter pollen dearth period of a humid subtropical climate increases rate of colony growth in colonies provisioned with a protein supplement. Experiments were conducted in late winter (9 February-9 March 2004) and mid-winter (19 January-8 February 2005). In both years, increased brood area, number of bees, and amount of protein supplement consumption were significantly greater in colonies receiving daily treatments of brood pheromone versus control colonies. Amount of extractable protein from hypopharyngeal glands measured in 2005 was significantly greater in bees from pheromone-treated colonies. These results suggest that brood pheromone may be used as a tool to stimulate colony growth in the southern subtropical areas of the United States where the package bee industry is centered and a large proportion of migratory colonies are overwintered.

  12. Effects of neonicotinoid imidacloprid exposure on bumble bee (Hymenoptera: Apidae) queen survival and nest initiation.

    Science.gov (United States)

    Wu-Smart, Judy; Spivak, Marla

    2018-02-08

    Neonicotinoids are highly toxic to insects and may systemically translocate to nectar and pollen of plants where foraging bees may become exposed. Exposure to neonicotinoids can induce detrimental sublethal effects on individual and colonies of bees and may have long-term impacts, such as impaired foraging, reduced longevity, and reduced brood care or production. Less well-studied are the potential effects on queen bumble bees that may become exposed while foraging in the spring during colony initiation. This study assessed queen survival and nest founding in caged bumble bees [Bombus impatiens (Cresson) (Hymenoptera: Apidae)] after chronic (18-d) dietary exposure of imidacloprid in syrup (1, 5, 10, and 25 ppb) and pollen (0.3, 1.7, 3.3, and 8.3 ppb), paired respectively. Here we show some mortality in queens exposed at all doses even as low as 1 ppb, and, compared with untreated queens, significantly reduced survival of treated queens at the two highest doses. Queens that survived initial imidacloprid exposure commenced nest initiation; however, they exhibited dose-dependent delay in egg-laying and emergence of worker brood. Furthermore, imidacloprid treatment affected other parameters such as nest and queen weight. This study is the first to show direct impacts of imidacloprid at field-relevant levels on individual B. impatiens queen survival and nest founding, indicating that bumble bee queens are particularly sensitive to neonicotinoids when directly exposed. This study also helps focus pesticide risk mitigation efforts and highlights the importance of reducing exposure rates in the early spring when bumble bee queens, and other wild bees are foraging and initiating nests. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  13. Euglossine bee communities in small forest fragments of the Atlantic Forest, Rio de Janeiro state, southeastern Brazil (Hymenoptera, Apidae Comunidade de abelhas Euglossina em pequenos fragmentos de Mata Atlântica no estado do Rio de Janeiro, sudeste do Brasil (Hymenoptera, Apidae

    Directory of Open Access Journals (Sweden)

    Willian Moura de Aguiar

    2012-06-01

    Full Text Available Euglossine bee communities in small forest fragments of the Atlantic Forest, Rio de Janeiro state, southeastern Brazil (Hymenoptera, Apidae. Euglossine bees are important pollinators in forests and agricultural areas. Although the structure of their communities is critically affected by anthropogenic disturbances, little is known about these bees in small forest fragments. The objectives of this study were to analyze the composition, abundance, and diversity of euglossine bee species in nine small fragments of different phytophysiognomies of the Atlantic Forest in southeastern Brazil, and to identify the environmental variables that may be related to the species composition of these communities. Males were sampled quarterly from May 2007 to May 2009 with aromatic traps containing methyl cinnamate, vanillin, eucalyptol, benzyl acetate, and methyl salicylate. A total of 1558 males, belonging to 10 species and three genera of Euglossina were collected. The richness ranged from five to seven species per fragment. Euglossa cordata, E. securigera, Eulaema nigrita e E. cingulata were common to all fragments studied. The diversity differed significantly among areas, ranging from H' = 1.04 to H' = 1.65. The precipitation, phytophysiognomy, and altitude had the highest relative importance over the species composition variation. The results presented in this study demonstrate that small forest fragments are able to support populations of euglossine bee species, most of which are widely distributed and reportedly tolerant to open and/or disturbed areas and suggest that the conservation of such areas is important, particularly in areas that are regenerating and in regions with agricultural matrices where these bees can act as important pollinatorsComunidade de abelhas Euglossina em pequenos fragmentos de Mata Atlântica no estado do Rio de Janeiro, sudeste do Brasil (Hymenoptera, Apidae. Abelhas Euglossina são importantes polinizadores nas florestas e em

  14. Ultrastructure of the intramandibular gland of workers and queens of the stingless bee, Melipona quadrifasciata.

    Science.gov (United States)

    Da Cruz-Landim, Carminda; Gracioli-Vitti, Luciana F; Abdalla, Fábio C

    2011-01-01

    The intramandibular glands of workers and queens of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae), at different ages and from different functional groups, were studied using light and transmission electron microscopy. The results demonstrated that these glands are composed of two types of secretory structures: 1.A hypertrophied epidermis on the dorsal side of the mandible that is an epithelial gland. 2. Free secretory cells filling the inner spaces of the appendices that constitute a unicellular gland. The epithelial gland is larger in the young (1-2-day-old workers), and the gland becomes involuted during the nurse worker stage. The unicellular glands of the workers posses some secretion during all of the studied phases, but secretory activity is more intensive in the foraging workers. Vesicles of secretion are absent in the unicellular glands of queens. These results demonstrate that these glands show functional adaptations in different castes corresponding to the functions of each caste.

  15. Revisión taxonómica del subgénero Micrandrena (Hymenoptera: Apoidea: Andrenidae: Andrena) de la Península Ibérica

    OpenAIRE

    Dardón Peralta, María José

    2011-01-01

    [ES]Las abejas (Hymenoptera: Apoidea) han sido clasificadas por diferentes autores siguiendo distintos criterios, aunque actualmente se emplea la propuesta de Michener (2007), quien establece siete familias: Andrenidae, Colletidae, Halictidae, Melittidae, Stenotritidae, Megachilidae y Apidae. La familia Andrenidae se divide en cuatro subfamilias: Oxaeinae, Andreninae, Alocandreninae y Panurginae. La subfamilia Andreninae está conformada por los géneros Ancyladrena Cockerell, 1930, Andrena...

  16. Odor Learning and Its Experience-Dependent Modulation in the South American Native Bumblebee Bombus atratus (Hymenoptera: Apidae)

    Science.gov (United States)

    Palottini, Florencia; Estravis Barcala, María C.; Farina, Walter M.

    2018-01-01

    Learning about olfactory stimuli is essential in bumblebees’ life since it is involved in orientation, recognition of nest sites, foraging efficiency and food yield for the colony as a whole. To evaluate associative learning abilities in bees under controlled environmental conditions, the proboscis extension response (PER) assay is a well-established method used in honey bees, stingless bees and successfully adapted to bumblebees of the genus Bombus. However, studies on the learning capacity of Bombus atratus (Hymenoptera: Apidae), one of the most abundant native species in South America, are non-existent. In this study, we examined the cognitive abilities of worker bees of this species, carrying out an olfactory PER conditioning experiment. Bumblebees were able to learn a pure odor when it was presented in paired association with sugared reward, but not when odor and reward were presented in an unpaired manner. Furthermore, if the bees were preexposed to the conditioned odor, the results differed depending on the presence of the scent either as a volatile in the rearing environment or diluted in the food. A decrement in learning performance results from the non-reinforced pre-exposure to the to-be-conditioned odor, showing a latent inhibition phenomenon. However, if the conditioned odor has been previously offered diluted in sugared reward, the food odor acts as a stimulus that improves the learning performance during PER conditioning. The native bumblebee B. atratus is thus a new hymenopteran species capable of being trained under controlled experimental conditions. Since it is an insect increasingly reared for pollination service, this knowledge could be useful in its management in crops. PMID:29755391

  17. Foraging pattern and harvesting of resources of subterranean stingless bee Geotrigona subterranea (Friese, 1901 (Hymenoptera: Apidae: Meliponini

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    Fernando Mendes Barbosa

    Full Text Available ABSTRACT Flight activity of bees is influenced both by environmental factors and by internal condition of the colonies. Information about external activity of bees is very important, because it provides data of the species biology, supplying subsidies for the use of these insects in the pollination of crops. The present work aim to evaluate the flight activity of Geotrigona subterranea (Friese, 1901 (Hymenoptera: Apidae in natural environment. This study was performed on the Instituto Federal do Norte de Minas Gerais, in the municipality Januária, Minas Gerais State. Two natural nests were observed. The activities of bees of the colonies were recorded three days each month, during the period of December 2011 to November 2012, totaling 924 observations. It was recorded the number of bees leaving and entering the nest, and the type of material transported by them for ten minutes each hour from 5 a.m. to 7 p.m. The bees entered the colony carrying pollen, resin, detritus and also without apparent material. The bees began external activities by 6 a.m. at 20°C and finished at 6 p.m. at 28.8°C. The peak of activity of G. subterranea occurs on schedule from 1 to 2 p.m. Even though G. subterranea makes their nests in underground, their foraging activities are very similar to others stingless bee species that usually nest on tree cavities or aerial places. This indicate that despite their particular nesting way the external factors as climatic ones will significantly modulate their foraging pattern in a daily and seasonal way.

  18. Rarely reported, widely distributed, and unexpectedly diverse: molecular characterization of mermithid nematodes (Nematoda: Mermithidae) infecting bumble bees (Hymenoptera: Apidae: Bombus) in the USA.

    Science.gov (United States)

    Tripodi, Amber D; Strange, James P

    2018-03-16

    Mermithid nematodes (Nematoda: Mermithida: Mermithidae) parasitize a wide range of both terrestrial and aquatic invertebrate hosts, yet are recorded in bumble bees (Insecta: Hymenoptera: Apidae: Bombus) only six times historically. Little is known about the specific identity of these parasites. In a single-season nationwide survey of internal parasites of 3646 bumble bees, we encountered six additional instances of mermithid parasitism in four bumble bee species and genetically characterized them using two regions of 18S to identify the specific host-parasite relationships. Three samples from the northeastern USA are morphologically and genetically identified as Mermis nigrescens, whereas three specimens collected from a single agricultural locality in the southeast USA fell into a clade with currently undescribed species. Nucleotide sequences of the V2-V6 region of 18S from the southeastern specimens were 2.6-3.0% divergent from one another, and 2.2-4.0% dissimilar to the nearest matches to available data. The dearth of available data prohibits positive identification of this parasite and its affinity for specific bumble bee hosts. By doubling the records of mermithid parasitism of bumble bee hosts and providing genetic data, this work will inform future investigations of this rare phenomenon.

  19. Biologia de Abertura e Fechamento do Tubo de Acesso de Nannotrigona testaceicornis (Lepeletier (Apidae: Meliponini

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    Luis Alves

    2015-04-01

    Abstract. The bee nest’s access tube relates to its defense, forage and physicochemical regulation through temperature and air circulation control. Therefore, it’s opening and closing causes a conflict (Trade-off in the size of the nest’s opening and the colony’s safety. This paper describes the process of opening and closing of the access tube in different times of the year and provides important information about the foraging behavior of Nannotrigona testaceicornis (Lepeletier. During this work, conducted in the Universidade Federal de Juiz de Fora campus (Minas Gerais, Brasil, 41 observations were performed, of which 27 took place during the hot and humid season (from October to March and 14 during the cold and dry season (from April to September, totaling 172 hours of observation. It was seen that the time spent in closing was significantly longer in the hot and humid season (χ² = 7.50; p < 0.0062, than in the cold and dry season. The longer closing time of the tube during the hot season and shorter in the cold season was regulated by the bee’s external activity rhythm, which is influenced by climatic factors. Thus, the opening and closing processes suffers alterations during the different seasons of the year. Accordingly, this paper shows important information about the species’ biology, information that may be used on its handling, increase its use on commercial activities and decrease the loss of workers during colony migration.

  20. Euglossine bee communities in small forest fragments of the Atlantic Forest, Rio de Janeiro state, southeastern Brazil (Hymenoptera, Apidae

    Directory of Open Access Journals (Sweden)

    Willian Moura de Aguiar

    2012-06-01

    Full Text Available Euglossine bee communities in small forest fragments of the Atlantic Forest, Rio de Janeiro state, southeastern Brazil (Hymenoptera, Apidae. Euglossine bees are important pollinators in forests and agricultural areas. Although the structure of their communities is critically affected by anthropogenic disturbances, little is known about these bees in small forest fragments. The objectives of this study were to analyze the composition, abundance, and diversity of euglossine bee species in nine small fragments of different phytophysiognomies of the Atlantic Forest in southeastern Brazil, and to identify the environmental variables that may be related to the species composition of these communities. Males were sampled quarterly from May 2007 to May 2009 with aromatic traps containing methyl cinnamate, vanillin, eucalyptol, benzyl acetate, and methyl salicylate. A total of 1558 males, belonging to 10 species and three genera of Euglossina were collected. The richness ranged from five to seven species per fragment. Euglossa cordata, E. securigera, Eulaema nigrita e E. cingulata were common to all fragments studied. The diversity differed significantly among areas, ranging from H' = 1.04 to H' = 1.65. The precipitation, phytophysiognomy, and altitude had the highest relative importance over the species composition variation. The results presented in this study demonstrate that small forest fragments are able to support populations of euglossine bee species, most of which are widely distributed and reportedly tolerant to open and/or disturbed areas and suggest that the conservation of such areas is important, particularly in areas that are regenerating and in regions with agricultural matrices where these bees can act as important pollinators

  1. Organization of the cysts in bee (Hymenoptera, Apidae testis: number of spermatozoa per cyst

    Directory of Open Access Journals (Sweden)

    Cruz-Landim Carminda da

    2001-01-01

    Full Text Available The morphology of the cyst cells in Apis mellifera Linné, 1758, Scaptotrigona postica Latreille, 1804, and Melipona bicolor bicolor Lepeletier, 1836 testis, as well as the average number of spermatic cells are reported. The data indicates a supporting and nourrishing role of the cyst cells to the developing cystocytes. The counts of immature spermatozoa in the cysts show an average of 202.8 ± 21.2 spermatozoa for A. mellifera, 117.4 ± 8.68 for S. postica and 88.8 ± 15.57 for M. bicolor, which predict the occurrence of 8 mitotic cycles in the cystocytes of A. mellifera and 7 in the meliponines, considering that only one spermatozoom originates of each final spermatogonium.

  2. Alelle number and heterozigosity for microsatellite loci in different stingless bee species (Hymenoptera: Apidae, Meliponini).

    Science.gov (United States)

    Francisco, Flávio de O; Brito, Rute M; Arias, Maria C

    2006-01-01

    In the present study we compare genetic characteristics (allele diversity and observed heterozygosity) of microsatellite loci, from three stingless bee species (Plebeia remota Holmberg, Partamona mulata Moure In Camargo and Partamona helleri Friese), amplified by using heterospecific primers originally designed for Melipona bicolor Lepeletier and Scaptotrigona postica Latreille. We analyzed 360 individuals of P. remota from 72 nests, 58 individuals of R. mulata from 58 nests, and 47 individuals of P. helleri from 47 nests. The three species studied showed low level of polymorphism for the loci amplified with primers derived from M. bicolor. However, for the loci amplified with primers derived from S. postica, only P. remota presented low level of polymorphism.

  3. Abelhas sem ferrão (Hymenoptera: Apidae, Meliponini da Estação Ecológica de Água Limpa, Cataguases-MG, Brasil.

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    Hugo Werneck

    2014-08-01

    Stingless Bees of the Estação Ecológica de Água Limpa, Cataguases-MG, Brazil Abstract. This study carried out a survey of nests of stingless bees present in the Estação Ecológica de Água Limpa (EEAL in the municipality of Cataguases, Minas Gerais. We found twenty-one nests belonging to nine species: Friesella schrottkyi (Friese, Melipona bicolor Lepeletier, Plebeia sp. 1, Plebeia sp. 2, Tetragona clavipes (Fabricius, Tetragona quadrangula (Fabricius, Tetragonisca angustula (Latreille, Trigona hyalinata (Lepeletier, Trigona spinipes (Fabricius. Twenty of the 21 nests were found in arboreal substrate. The fauna of stingless bees observed in EEAL is represented by a low number of species. Although the local vegetation has the potential to provide substrates for nesting and floral resources, the low species richness may be related to the fact that the EEAL is an isolated forest remnant.

  4. Effects of brood pheromone (SuperBoost) on consumption of protein supplement and growth of honey bee (Hymenoptera: Apidae) colonies during fall in a northern temperate climate.

    Science.gov (United States)

    Sagili, Ramesh R; Breece, Carolyn R

    2012-08-01

    Honey bee, Apis mellifera L. (Hymenoptera: Apidae), nutrition is vital for colony growth and maintenance of a robust immune system. Brood rearing in honey bee colonies is highly dependent on protein availability. Beekeepers in general provide protein supplement to colonies during periods of pollen dearth. Honey bee brood pheromone is a blend of methyl and ethyl fatty acid esters extractable from cuticle of honey bee larvae that communicates the presence of larvae in a colony. Honey bee brood pheromone has been shown to increase protein supplement consumption and growth of honey bee colonies in a subtropical winter climate. Here, we tested the hypothesis that synthetic brood pheromone (SuperBoost) has the potential to increase protein supplement consumption during fall in a temperate climate and thus increase colony growth. The experiments were conducted in two locations in Oregon during September and October 2009. In both the experiments, colonies receiving brood pheromone treatment consumed significantly higher protein supplement and had greater brood area and adult bees than controls. Results from this study suggest that synthetic brood pheromone may be used to stimulate honey bee colony growth by stimulating protein supplement consumption during fall in a northern temperate climate, when majority of the beekeepers feed protein supplement to their colonies.

  5. The Effects of the Insect Growth Regulators Methoxyfenozide and Pyriproxyfen and the Acaricide Bifenazate on Honey Bee (Hymenoptera: Apidae) Forager Survival.

    Science.gov (United States)

    Fisher, Adrian; Colman, Chet; Hoffmann, Clint; Fritz, Brad; Rangel, Juliana

    2018-04-02

    The honey bee (Apis mellifera L. (Hymenoptera: Apidae)) contributes an essential role in the U.S. economy by pollinating major agricultural crops including almond, which depends entirely on honey bee pollination for successful nut set. Almond orchards are often treated with pesticides to control a variety of pests and pathogens, particularly during bloom. While the effects to honey bee health of some insecticides, particularly neonicotinoids, have received attention recently, the impact of other types of insecticides on honey bee health is less clear. In this study, we examined the effects to honey bee forager survival of three non-neonicotinoid pesticides widely used during the 2014 California almond bloom. We collected foragers from a local apiary and exposed them to three pesticides at the label dose, or at doses ranging from 0.5 to 3 times the label dose rate. The selected pesticides included the insect growth regulators methoxyfenozide and pyriproxyfen, and the acaricide bifenazate. We simulated field exposure of honey bees to these pesticides during aerial application in almond orchards by using a wind tunnel and atomizer set up with a wind speed of 2.9 m/s. Experimental groups consisting of 30-40 foragers each were exposed to either untreated controls or pesticide-laden treatments and were monitored every 24 hr over a 10-d period. Our results revealed a significant negative effect of all pesticides tested on forager survival. Therefore, we suggest increased caution in the application of these pesticides in almond orchards or any agricultural crop during bloom to avoid colony health problems.

  6. Removal of drone brood from Apis mellifera (Hymenoptera: Apidae) colonies to control Varroa destructor (Acari: Varroidae) and retain adult drones.

    Science.gov (United States)

    Wantuch, Holly A; Tarpy, David R

    2009-12-01

    The parasitic mite Varroa destructor Anderson & Trueman (Acari: Varroidae) has plagued European honey bees, Apis mellifera L. (Hymenoptera: Apidae), in the Americas since its introduction in the 1980s. For many years, these mites were sufficiently controlled using synthetic acaricides. Recently, however, beekeepers have experienced increased resistance by mites to chemical pesticides, which are also known to leave residues in hive products such as wax and honey. Thus there has been increased emphasis on nonchemical integrated pest management control tactics for Varroa. Because mites preferentially reproduce in drone brood (pupal males), we developed a treatment strategy focusing on salvaging parasitized drones while removing mites from them. We removed drone brood from colonies in which there was no acaricidal application and banked them in separate "drone-brood receiving" colonies treated with pesticides to kill mites emerging with drones. We tested 20 colonies divided into three groups: 1) negative control (no mite treatment), 2) positive control (treatment with acaricides), and 3) drone-brood removal and placement into drone-brood receiving colonies. We found that drone-brood trapping significantly lowered mite numbers during the early months of the season, eliminating the need for additional control measures in the spring. However, mite levels in the drone-brood removal group increased later in the summer, suggesting that this benefit does not persist throughout the entire season. Our results suggest that this method of drone-brood trapping can be used as an element of an integrated control strategy to control varroa mites, eliminating a large portion of the Varroa population with limited chemical treatments while retaining the benefits of maintaining adult drones in the population.

  7. Abelhas Euglossina (Hymenoptera, Apidae coletadas em uma monocultura de eucalipto circundada por Cerrado em Urbano Santos, Maranhão, Brasil Euglossina bees (Hymenoptera, Apidae collected in an eucalyptus monoculture surounded by Cerrado, Urbano Santos, MA, Brazil

    Directory of Open Access Journals (Sweden)

    Fernanda N. Mendes

    2008-09-01

    Full Text Available Machos de Euglossina foram coletados por meio de iscas-odores de benzoato de benzila, eucaliptol, eugenol, salicilato de metila e vanilina em uma monocultura de eucalipto circundada por cerrado, no município de Urbanos Santos, Maranhão. As coletas foram realizadas mensalmente, de abril de 2001 a abril de 2002, entre 8h e 16h, totalizando 96 horas de amostragem. Foram coletados 58 indivíduos de 3 gêneros e 10 espécies. Euglossa Latreille, 1802 foi o gênero mais abundante, seguido por Eufriesea Cockerell, 1909 e Eulaema Lepeletier, 1841. As espécies mais freqüentes foram Euglossa cordata (Linnaeus, 1758, Euglossa gaianii Dressler, 1982 e Euglossa modestior (Dressler, 1982. Eucaliptol foi a essência mais atrativa. As maiores freqüências de visitas ocorreram no período da manhã e as maiores abundâncias em setembro, no período de estiagem, e em dezembro, no período chuvoso.Males of Euglossina bees were collected in benzil benzoate, eucaliptol, eugenol, methyl salicylate and vanillin scent baits in an eucalyptus monoculture surrounded by cerrado, located in the municipality of Urbano Santos, Maranhão. The collections were carried out monthly, from April 2001 to April 2002, between 8 a.m. and 4 p.m., totaling 96 hours of sampling, resulting in 58 individuals of 3 genera and 10 species. Euglossa Latreille, 1802 was the most abundant genus, followed by Eufriesea Cockerell, 1909 and Eulaema Lepeletier, 1841. The most frequent species were Euglossa cordata (Linnaeus, 1758, Euglossa gaianii Dressler, 1982 and Euglossa modestior (Dressler, 1982. Eucaliptol was the most attractive chemical bait. The highest frequencies of visits were in the morning and the highest abundance in September, in the drought period, and December, in the rainy period.

  8. A Bio-Economic Case Study of Canadian Honey Bee (Hymenoptera: Apidae) Colonies: Marker-Assisted Selection (MAS) in Queen Breeding Affects Beekeeper Profits

    Science.gov (United States)

    Baylis, Kathy; Hoover, Shelley E.; Currie, Rob W.; Melathopoulos, Andony P.; Pernal, Stephen F.; Foster, Leonard J.; Guarna, M. Marta

    2017-01-01

    Abstract Over the past decade in North America and Europe, winter losses of honey bee (Hymenoptera: Apidae) colonies have increased dramatically. Scientific consensus attributes these losses to multifactorial causes including altered parasite and pathogen profiles, lack of proper nutrition due to agricultural monocultures, exposure to pesticides, management, and weather. One method to reduce colony loss and increase productivity is through selective breeding of queens to produce disease-, pathogen-, and mite-resistant stock. Historically, the only method for identifying desirable traits in honey bees to improve breeding was through observation of bee behavior. A team of Canadian scientists have recently identified markers in bee antennae that correspond to behavioral traits in bees and can be tested for in a laboratory. These scientists have demonstrated that this marker-assisted selection (MAS) can be used to produce hygienic, pathogen-resistant honey bee colonies. Based on this research, we present a beekeeping case study where a beekeeper’s profit function is used to evaluate the economic impact of adopting colonies selected for hygienic behavior using MAS into an apiary. Our results show a net profit gain from an MAS colony of between 2% and 5% when Varroa mites are effectively treated. In the case of ineffective treatment, MAS generates a net profit benefit of between 9% and 96% depending on the Varroa load. When a Varroa mite population has developed some treatment resistance, we show that MAS colonies generate a net profit gain of between 8% and 112% depending on the Varroa load and degree of treatment resistance. PMID:28334400

  9. Variação do tamanho corporal de machos de Eulaema nigrita Lepeletier (Hymenoptera, Apidae, Euglossini. Resposta materna à flutuação de recursos?

    Directory of Open Access Journals (Sweden)

    Peruquetti Rui Carlos

    2003-01-01

    Full Text Available It was compared body size (measured as intertegular span variance of trapped-males of Eulaema nigrita Lepeletier, 1841 sampled in dry (July, August and September and wet (December, January and February seasons of the years 1988/89 and 1994/95 in Viçosa (Minas Gerais, Brazil. It was also compared the body size variance between males and females sampled in three nests found in São Carlos (São Paulo, Brazil and between these males and trapped ones. The smaller male (6.4 mm was sampled in June and the bigger (8.9 mm in July 1994, but the majority (32.3% showed an intertegular span ranging from 7.8 to 8.0mm. The observed variance in body size was similar between males sampled in nests and trapped-males and the variance found inside a nest was similar between the sexes. However, males sampled in 1988/89 were bigger than 1994/95 males and males sampled in nests were smaller than trapped-males. The variance of the body size of males did differ between 1988/89 and 1994/95. In the first period differences between males sampled in dry or wet season was not observed, but in 1994/95 period the males sampled in dry season showed significantly greater variance in body size than males sampled in wet season. The body size variance did not differ between the wet seasons of 1988/89 and 1994/95. The body size variation, measured as the coefficient of variation in intertegular span, did not differ between males of E. nigrita (CV = 4.3% and ground-nesting bees. These results show that the variation in body size of males of E. nigrita reflects that one found inside the nests of this bee, being similar among males and females. This variation is expected as result of ecological factors influences the nesting females. El Niño climatic events alone or in association with the lack of local food resources due to deforestation and presence of monocultures might play a role in observed body size variation. However this hypothesis is not sufficient to explain the observed

  10. On the identity of the adventive species of Eufriesea Cockerell in the USA: systematics and potential distribution of the coerulescens species group (Hymenoptera: Apidae)

    Science.gov (United States)

    In the summer of 2010, two male specimens of the neotropical orchid bee genus Eufriesea Cockerell were collected in the Guadalupe Mountains of western Texas and southeastern New Mexico, USA. We tentatively identified them as E. coerulescens (Lepeletier de Saint Fargeau) because of the uncertainty su...

  11. Hymenoptera of Afghanistan and the central command area of operations: assessing the threat to deployed U.S. service members with insect venom hypersensitivity.

    Science.gov (United States)

    Turbyville, Joseph C; Dunford, James C; Nelson, Michael R

    2013-01-01

    Insect venom hypersensitivity can pose a threat to personnel deployed to a combat zone but the exposure risk in Afghanistan is currently unknown. This study was designed to assess the threat of Hymenoptera stings and associated allergic reactions in Afghanistan. Hymenoptera species were collected during a deployment to southern Afghanistan from June 2010 through January 2011. The literature was also reviewed to determine species of medically important Hymenoptera recorded in the region. The U.S. Army theater electronic medical data system was mined for ICD-9 codes associated with insect stings to determine the number of theater medical clinic encounters addressing insect sting reactions. Three species of flying hymenoptera were commonly encountered during the study period: Vespa orientalis L., Polistes wattii Cameron, and Vespula germanica (F.). A literature review also confirms the presence of honeybees (Apidae), numerous velvet ant (Mutillidae) species, and various ant (Formicidae) species all capable of stinging. No evidence was identified to suggest that fire ants (Solenopsis ssp.) are a threat in the region. Based on electronic medical records from the U.S. Central Command area of operations over a 2-year period, roughly 1 in 500 clinic visits involved a patient with a diagnosis of insect bite or sting. Cross-reactive members of all five flying Hymenoptera species commonly assessed for in Hymenoptera allergy evaluations are present in Afghanistan. The review of in-theater medical records confirms that insect stings pose an environmental threat to deployed service members.

  12. As abelhas eussociais (Hymenoptera, Apidae visitantes florais em um ecossistema de dunas continentais no médio Rio São Francisco, Bahia, Brasil

    Directory of Open Access Journals (Sweden)

    Edinaldo Luz das Neves

    2002-01-01

    Full Text Available Highly eusocial bees (Hymenoptera, Apidae flower visitors in a continental sand dune ecosystem from the medium São Francisco River, Bahia, Brazil. A community of highly eusocial bees in sand dunes, covered with caatinga vegetation, in the medium São Francisco River, Bahia (10º47' 37"S and 42º49' 25"W was studied. The local climate is semi arid and hot, with mean temperature of 25.7 ºC and annual precipitation of 653.8 mm. Censuses took place every two months, from February to December of 2000. The bees were sampled on flowers with entomological nets, from 6:00 a.m. to 5:00 p.m. A total of 2,147 individuals of eight species of Apinae were found, of which Apis mellifera Linnaeus (40.2%, Trigona spinipes (Fabricius (28.7% and Frieseomelitta silvestri languida Moure (14.7% were the predominant species. The diversity was H' = 1.53 and the evenness E' = 0.73. The bees were active during the whole year, but there was a significant variation in the monthly abundance of individuals (c2= 799.55; df= 35; p<0.0001. The daily activity was greater between 6:00 a.m. and 8:00 a.m. The low bee diversity observed is a consequence of the low richness of botanical species and of the small amount of sites for the bees' nests. The community of highly eusocial bees from the dunes presents organization patterns similar to those observed in other caatinga areas, albeit with some particularities.

  13. Diversidade de abelhas (Hymenoptera, Apidae) ao longo de um gradiente latitudinal na Mata Atlântica

    OpenAIRE

    Gonçalves,Rodrigo Barbosa; Brandão,Carlos Roberto Ferreira

    2008-01-01

    A Mata Atlântica é um dos ambientes mais ricos e ameaçados do mundo, o que deveria ter estimulado em muito o estudo e a conservação do Bioma, mas a fauna de Hymenoptera permanece ainda relativamente pouco conhecida. Em especial, a fauna de abelhas da floresta ombrófila densa é pouco estudada em comparação à fauna das áreas abertas brasileiras. O projeto temático "Biodiversidade de Hymenoptera e Isoptera: riqueza e diversidade ao longo de um gradiente latitudinal na Mata Atlântica - a floresta...

  14. Número de alelos XO em uma população de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae Number of XO alleles in a population of Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae

    Directory of Open Access Journals (Sweden)

    Davi S. Aidar

    2001-12-01

    Full Text Available The number of xo alleles of a population of stingless bees can be estimated by observing the male proportion of the first brood comb produced by a new queen (about 40 Fl bees and the total number of colonies. A proportion of 50% diploid males indicates insemination of the queen by a single male with some xo sex allele: 25% diploid male would indicate insemination by two males (one with some xo as the queen and another with different one. Diploidy was confirmed by cytology of the testis. The original population (78 colonies of Ribeirão Preto, State S. Paulo, Brazil, had 8 xo sex alleles. From this population, 10 colonies were orphaned and taken to the Serra do Mar (Sea Mountains at Espirito Santo State (1200 km far in order that the virgin queen of each colony would cross with males of this region. Ten days after the nine hives (one was lost were brought back to Ribeirão Preto. No one of them produced diploid males. New samples took from all the Meliponary revealed that the number of xo sex alleles to have increased to 17,3. Than, this methodology can be used to increase the genetic variability of the stingless bees populations.

  15. A Bio-Economic Case Study of Canadian Honey Bee (Hymenoptera: Apidae) Colonies: Marker-Assisted Selection (MAS) in Queen Breeding Affects Beekeeper Profits.

    Science.gov (United States)

    Bixby, Miriam; Baylis, Kathy; Hoover, Shelley E; Currie, Rob W; Melathopoulos, Andony P; Pernal, Stephen F; Foster, Leonard J; Guarna, M Marta

    2017-06-01

    Over the past decade in North America and Europe, winter losses of honey bee (Hymenoptera: Apidae) colonies have increased dramatically. Scientific consensus attributes these losses to multifactorial causes including altered parasite and pathogen profiles, lack of proper nutrition due to agricultural monocultures, exposure to pesticides, management, and weather. One method to reduce colony loss and increase productivity is through selective breeding of queens to produce disease-, pathogen-, and mite-resistant stock. Historically, the only method for identifying desirable traits in honey bees to improve breeding was through observation of bee behavior. A team of Canadian scientists have recently identified markers in bee antennae that correspond to behavioral traits in bees and can be tested for in a laboratory. These scientists have demonstrated that this marker-assisted selection (MAS) can be used to produce hygienic, pathogen-resistant honey bee colonies. Based on this research, we present a beekeeping case study where a beekeeper's profit function is used to evaluate the economic impact of adopting colonies selected for hygienic behavior using MAS into an apiary. Our results show a net profit gain from an MAS colony of between 2% and 5% when Varroa mites are effectively treated. In the case of ineffective treatment, MAS generates a net profit benefit of between 9% and 96% depending on the Varroa load. When a Varroa mite population has developed some treatment resistance, we show that MAS colonies generate a net profit gain of between 8% and 112% depending on the Varroa load and degree of treatment resistance. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America.

  16. A molecular phylogeny of the stingless bee genus Melipona (Hymenoptera: Apidae).

    Science.gov (United States)

    Ramírez, Santiago R; Nieh, James C; Quental, Tiago B; Roubik, David W; Imperatriz-Fonseca, Vera L; Pierce, Naomi E

    2010-08-01

    Stingless bees (Meliponini) constitute a diverse group of highly eusocial insects that occur throughout tropical regions around the world. The meliponine genus Melipona is restricted to the New World tropics and has over 50 described species. Melipona, like Apis, possesses the remarkable ability to use representational communication to indicate the location of foraging patches. Although Melipona has been the subject of numerous behavioral, ecological, and genetic studies, the evolutionary history of this genus remains largely unexplored. Here, we implement a multigene phylogenetic approach based on nuclear, mitochondrial, and ribosomal loci, coupled with molecular clock methods, to elucidate the phylogenetic relationships and antiquity of subgenera and species of Melipona. Our phylogenetic analysis resolves the relationship among subgenera and tends to agree with morphology-based classification hypotheses. Our molecular clock analysis indicates that the genus Melipona shared a most recent common ancestor at least approximately 14-17 million years (My) ago. These results provide the groundwork for future comparative analyses aimed at understanding the evolution of complex communication mechanisms in eusocial Apidae. Copyright 2010 Elsevier Inc. All rights reserved.

  17. Detoxification and stress response genes expressed in a western North American bumble bee, Bombus huntii (Hymenoptera: Apidae).

    Science.gov (United States)

    Xu, Junhuan; Strange, James P; Welker, Dennis L; James, Rosalind R

    2013-12-12

    The Hunt bumble bee (Bombus huntii Greene, Hymenoptera: Apidae) is a holometabolous, social insect important as a pollinator in natural and agricultural ecosystems in western North America. Bumble bees spend a significant amount of time foraging on a wide variety of flowering plants, and this activity exposes them to both plant toxins and pesticides, posing a threat to individual and colony survival. Little is known about what detoxification pathways are active in bumble bees, how the expression of detoxification genes changes across life stages, or how the number of detoxification genes expressed in B. huntii compares to other insects. We found B. huntii expressed at least 584 genes associated with detoxification and stress responses. The expression levels of some of these genes, such as those encoding the cytochrome P450s, glutathione S-transferases (GSTs) and glycosidases, vary among different life stages to a greater extent than do other genes. We also found that the number of P450s, GSTs and esterase genes expressed by B. huntii is similar to the number of these genes found in the genomes of other bees, namely Bombus terrestris, Bombus impatiens, Apis mellifera and Megachile rotundata, but many fewer than are found in the fly Drosophila melanogaster. Bombus huntii has transcripts for a large number of detoxification and stress related proteins, including oxidation and reduction enzymes, conjugation enzymes, hydrolytic enzymes, ABC transporters, cadherins, and heat shock proteins. The diversity of genes expressed within some detoxification pathways varies among the life stages and castes, and we typically identified more genes in the adult females than in larvae, pupae, or adult males, for most pathways. Meanwhile, we found the numbers of detoxification and stress genes expressed by B. huntii to be more similar to other bees than to the fruit fly. The low number of detoxification genes, first noted in the honey bee, appears to be a common phenomenon among bees

  18. Revisão das espécies de Melipona do grupo fuliginosa (Hymenoptera, Apoidea, Apidae, Meliponini Revision of the species of Melipona of the fuliginosa group (Hymenoptera, Apoidea, Apidae, Meliponini

    Directory of Open Access Journals (Sweden)

    João M. F. Camargo

    2008-09-01

    Full Text Available Três espécies são reconhecidas: Melipona (Michmelia fuliginosa Lepeletier, 1836, de larga distribuição, do Suriname e Guiana Francesa até o sudeste do Brasil e noroeste da Argentina, M. (Michmelia titania Gribodo, 1893 (revalidado, endêmica do oeste da Amazônia, e M. (Michmelia fallax sp. nov., do noroeste do Equador até a América Central. Melipona fuliginosa distingue-se pela pilosidade dos tergos metassomáticos II-V, tanto do macho como da operária, densa e plumosa, e pelo primeiro tarsômero da perna III do macho mais largo que longo. Em M. titania e M. fallax sp. nov., a pilosidade dos tergos II-V é escassa e simples, não-plumosa, e o primeiro tarsômero tão longo quanto largo ou mais longo que largo. Operárias de Melipona titania e M. fallax sp. nov. separam-se pela forma do penicilo, que é fortemente sinuoso em M. titania, e nos machos de M. fallax sp. nov. as órbitas internas dos olhos são paralelas, enquanto em M. titania as órbitas são convergentes embaixo. Novos registros geográficos, dados bionômicos e uma chave para identificação das espécies são apresentados. Adicionalmente, são feitos comentários sobre o padrão biogeográfico e sobre as glândulas tergais das rainhas.Three species are recognized: Melipona (Michmelia fuliginosa Lepeletier, 1836, widely distributed, from Suriname and French Guiana to southeastern Brazil and northwestern Argentina, M. (Michmelia titania Gribodo, 1893 (revalidated, endemic to western Amazon and M. (Michmelia fallax sp. nov., from northwestern Ecuador to Central America. Melipona fuliginosa differs from the other species by the pilosity of metasomal terga II-V, dense and plumose, in both male and workers, and the first tarsomere of leg III of the male wider than long. In M. titania and M. fallax sp. nov., the pilosity of terga II-V is scarce and simple, non-plumose, and the first tarsomere of leg III of the male is as long as wide or longer. Workers of Melipona titania and

  19. O Efeito do Fogo sobre a Comunidade de Abelhas Euglossini (Hymenoptera: Apidae em Floresta de Transição Cerrado-Amazônia (Mato Grosso, Brasil

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    Nubia Giehl

    2013-12-01

    Abstract. We evaluated the effects of induced burned on Euglossini bee assemblages (Hymenoptera: Apidae in a transitional area between Cerrado and Amazonia, eastern Mato Grosso, Brazil. We determinate abundances, richness and composition of Euglossini in three plots: control plot (unburned, plot burned each year since 2004 (intermediate degradation, plot burned each three years since 2004 (high degradation. We tested the hypothesis that two burned plots present lower male abundances, less species richness and different species composition in comparison with the control plot. We collected male bees actively and passively by using six pure fragrances: β-ionona, benzoato de benzila, geraniol, fenil-etil-acetato, salicilato de metila e vanilina. We collected seven species with no differences in male abundances among three plots (F (2, 12= 0.150; p= 0.8. Estimated richness species in control the plot was higher than the plot burned each three years (12 ± 3.8; 4± 2, respectively, while plot burned each year showed intermediate richness (8 ± 4.35 and higher than plot burned each three years. Cluster Analysis (UPGMA revealed significant differences in species composition of the triennial fire area to the other two areas. Our results suggest that fire occurring with different frequencies in transitional forest promote decreases in richness of species and modifications in species composition. These modifications were clearer in plot more degraded (burned each three years and induce deleterious effects on orchid bee assemblage.

  20. Variation and genetic structure of Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations based on ISSR pattern.

    Science.gov (United States)

    Nascimento, Marcília A; Batalha-Filho, Henrique; Waldschmidt, Ana M; Tavares, Mara G; Campos, Lucio A O; Salomão, Tânia M F

    2010-04-01

    For a study of diversity and genetic structuring in Melipona quadrifasciata, 61 colonies were collected in eight locations in the state of Minas Gerais, Brazil. By means of PCR analysis, 119 ISSR bands were obtained, 80 (68%) being polymorphic. H(e) and H (B) were 0.20 and 0.16, respectively. Two large groups were obtained by the UPGMA method, one formed by individuals from Januária, Urucuia, Rio Vermelho and Caeté and the other by individuals from São João Del Rei, Barbacena, Ressaquinha and Cristiano Otoni. The Φst and θ(B) values were 0.65 and 0.58, respectively, thereby indicating high population structuring. UPGMA grouping did not reveal genetic structuring of M. quadrifasciata in function of the tergite stripe pattern. The significant correlation between dissimilarity values and geographic distances (r = 0.3998; p Melipona quadrifasciata population structuring, possibly applicable to the studies of other bee species.

  1. Nidificação e forrageamento de Centris (Ptilotopus scopipes Friese (Hymenoptera, Apidae Nesting biology and foraging behavior of Centris (Ptilotopus scopipes Friese (Hymenoptera, Apidae

    Directory of Open Access Journals (Sweden)

    Maria Cristina Gaglianone

    2001-07-01

    Full Text Available Centris (Ptilotopus are large bees and important pollinators in the Neotropical region. Its biology and behavior is still poorly known and only a few observations have been published. In this paper, observations on the biology of C. (Ptilotopus scopipes Friese, 1899, a species that occurs in the "cerrados" of Brazil, are presented. The study was conducted in the Estação Ecológica de Jataí, in Luiz Antônio, São Paulo, during the active period of the adults (November through April. Females collected pollen from flowers of Solanaceae and Caesalpiniaceae, by vibration, and floral oils from Malpighiaceae belonging to the genera Byrsonima, Banisteriopsis, Stigmaphyllon and Peixotoa. Floral resources are carried in the hind leg scopae to the nests, which are excavated in the walls of epigeous nests of the termite Procornitermes araujoi Emerson, 1952 (Termitidae, Nasutitermitinae. The main tunnel entered the termite nest at an ascending angle ending in a vestibulum. Two parallel, vertical burrows forming linear series of cells descended from the vestibulum. The upper cell (the nearest to the nest entrance was filled with soil. This might represent a protection against parasites. During the study, one female of Acanthopus excellens Schrottky, 1902 (Apidae, Ericrocidini, a cleptoparasitic bee, was captured when leaving one of the nests. A male of C. scopipes emerged eleven months after cell closure, suggesting a univoltine cycle for this species.

  2. Development of an Application Programming Interface for Depletion Analysis (APIDA)

    International Nuclear Information System (INIS)

    Lago, Daniel; Rahnema, Farzad

    2017-01-01

    Highlights: • APIDA an Application Programming Interface tool for Depletion Analysis. • APIDA employs a matrix exponential method and a linear chain method. • A burnup solver to couple to neutron transport solvers in lattice depletion or reactor core analysis codes. - Abstract: A new utility has been developed with extensive capabilities in identifying nuclide decay and transmutation characteristics, allowing for accurate and efficient tracking of the change in isotopic concentrations in nuclear reactor fuel over time when coupled with a transport solution method. This tool, named the Application Programming Interface for Depletion Analysis (APIDA), employs both a matrix exponential method and a linear chain method to solve for the end-of-time-step nuclide concentrations for all isotopes relevant to nuclear reactors. The Chebyshev Rational Approximation Method (CRAM) was utilized to deal with the ill-conditioned matrices generated during lattice depletion calculations, and a complex linear chain solver was developed to handle isotopes reduced from the burnup matrix due to either radioactive stability or a sufficiently low neutron-induced reaction cross section. The entire tool is housed in a robust but simple application programming interface (API). The development of this API allows other codes, particularly numerical neutron transport solvers, to incorporate APIDA as the burnup solver in a lattice depletion code or reactor core analysis code in memory, without the need to write or read from the hard disk. The APIDA code was benchmarked using several decay and transmutation chains. Burnup solutions produced by APIDA were shown to provide material concentrations comparable to the analytically solved Bateman equations – well below 0.01% relative error for even the most extreme cases using isotopes with vastly different decay constants. As a first order demonstration of the API, APIDA was coupled with the transport solver in the SERPENT code for a fuel pin

  3. Especies nuevas de abejas de Cuba y La Española (Hymenoptera: Colletidae, Megachilidae, Apidae

    Directory of Open Access Journals (Sweden)

    Julio A. Genaro

    2001-12-01

    Full Text Available Se describen e ilustran cinco especies nuevas de abejas antillanas: Collectes granpiedrensis n. sp. (Cuba (Colletidae; Osmia (Diceratosmia stangei n. sp. (República Dominicana; Coelioxys (Cyrtocoelioxys alayoi n. sp. (Cuba; C. (Boreocoelioxys sannicolarensis n. sp. (Cuba (Megachilidae y Triepeolus nisibonensis n. sp. (República Dominicana (ApidaeFive new species of Antillean bees are described and illustrated: Colletes granpiedrensis n. sp. (Cuba (Colletidae is charaterized as follows: Head and mesosoma black, legs and metasoma brown. Dense brown hairs on head and mesosoma; white on frons and metasomal terga. Clypeus, frons and mesosoma with large punctures, lesser on vertex and metasoma. Malar space more wide than long. Male and female slightly similar, except in the apical margin of clypeus, supraclipeal area, and color of the pubescence on legs and sterna; Osmia (Diceratosmia stangei n. sp. (Dominican Republic (Megachilidae is charaterized as follows: Dark metallic green, metasoma black with metallic green reflections. Pubescence light; body with large, closed punctures. Female with violet reflections in tergum III and mandible tridentate; Coelioxys (Cyrtocoelioxys alayoi n. sp. (Cuba (Megachilidae is charaterized as follows: Female black, except basal area of mandibles, tegula, legs, lateral area of tergum I and sterna, reddish brown. Posterior margin of scutellum rounded. Apex of tergum VI with spine curved up. Sternum VI fringed with short, closed setae, and the apex with short spine; Coelioxys (Boreocoelioxys sannicolarensis n. sp. (Cuba (Megachilidae is charaterized as follows: Black, except antenna and tegula brown; legs and sterna reddish brown. Clypeal margin straight in profile. Gradular grooves on metasomal terga II and III distinct medially. Fovea on metasomal tergum II of male deep and short, and Triepeolus nisibonensis n. sp. (Dominican Republic (Apidae is charaterized as follows: Dorsal pubescence (short and dense on mesosoma

  4. ABELHAS EUGLOSSINA (HYMENOPTERA: APIDAE ASSOCIADAS À MONOCULTURA DE EUCALIPTO NO CERRADO MATO-GROSSENSE

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    Silva do Nascimento

    2015-04-01

    Full Text Available O objetivo deste estudo foi avaliar a composição de abelhas Euglossina em três áreas distintas, com monocultura de eucalipto de diferentes idades, utilizando a vegetação nativa como controle, com base nos aspectos de riqueza e abundância. O trabalho foi realizado em três propriedades particulares, localizadas na região Sudoeste de Mato Grosso, em monocultura de eucaliptos de diferentes idades e vegetação nativa (Cerrado. As coletas foram realizadas mensalmente, de dezembro de 2011 a março de 2012, utilizando-se seis essências: eugenol, eucaliptol, vanilina, benzoato de benzila, salicitato de metila e acetato de benzila, das 8 às 16 h. Foram coletados 430 espécimes, de quatro gêneros e 18 espécies. Eulaema nigrita Lepeletier, 1841, Euglossa melanotricha Moure, 1967 e Eulaema cingulata Fabricius, 1804 foram as espécies mais abundantes e comuns a todas as áreas estudadas. A área com maior abundância de abelhas foi ApS (166 indivíduos e com maior riqueza, a Tol (14 espécies. A composição de espécies foi semelhante nas áreas analisadas, e a abundância apresentou dissimilaridade entre a Tol e as áreas SanR e ApS. A área AC (área-controle apresentou maior abundância (147 e riqueza (n = 15 em relação à monocultura de eucalipto.

  5. Fertility signals in the bumblebee Bombus terrestris (Hymenoptera: Apidae)

    Science.gov (United States)

    Sramkova, A.; Schulz, C.; Twele, R.; Francke, W.; Ayasse, M.

    2008-06-01

    In eusocial Hymenoptera, queen control over workers is probably inseparable from the mechanism of queen recognition. In primitively eusocial bumblebees ( Bombus), worker reproduction is controlled not only by the presence or absence of a dominant queen but also by other dominant workers. Furthermore, it was shown that the queen dominance is maintained by pheromonal cues. We investigated whether there is a similar odor signal released by egg-laying queens and workers that may have a function as a fertility signal. We collected cuticular surface extracts from nest-searching and breeding Bombus terrestris queens and workers that were characterized by their ovarian stages. In chemical analyses, we identified 61 compounds consisting of aldehydes, alkanes, alkenes, and fatty acid esters. Nest-searching queens and all groups of breeding females differed significantly in their odor bouquets. Furthermore, workers before the competition point (time point of colony development where workers start to develop ovaries and lay eggs) differed largely from queens and all other groups of workers. Breeding queens showed a unique bouquet of chemical compounds and certain queen-specific compounds, and the differences toward workers decrease with an increasing development of the workers’ ovaries, hinting the presence of a reliable fertility signal. Among the worker groups, the smallest differences were found after the competition point. Egg-laying females contained higher total amounts of chemical compounds and of relative proportions of wax-type esters and aldehydes than nest-searching queens and workers before the competition point. Therefore, these compounds may have a function as a fertility signal present in queens and workers.

  6. Notes on Apidae and Vespidae (Hymenoptera) Species Collected by Bait Traps in OrganicVineyard and Orchards of Kemalpaşa (İzmir), Western Turkey

    OpenAIRE

    ÜZÜM, Ahu; TANYERİ, Rukiye; GÜLPERÇİN, Nilay; TEZCAN, Serdar; YILDIRIM, Erol

    2010-01-01

    Hymenoptera species collected by bait traps during the months of June-October in organic vineyard and orchards in Kemalpaşa district, (İzmir) of Western Turkey were evaluated in this study. As a result, six species belonging 2007 to two families of Hymenoptera were determined. Those were Apis mellifera Linnaeus, 1758, Vespula germanica (Fabricius, 1793), Vespa crabro Linnaeus, 1758, Vespa orientalis Linnaeus, 1771, Polistes dominulus (Christ, 1791) and Polistes gallicus (Linnaeus, 1767). Amon...

  7. A scent shield to survive: identification of the repellent compounds secreted by the male offspring of the cuckoo bumblebee Bombus vestalis

    Czech Academy of Sciences Publication Activity Database

    Lhomme, P.; Ayasse, M.; Valterová, Irena; Lecocq, T.; Rasmont, P.

    2015-01-01

    Roč. 157, č. 3 (2015), s. 263-270 ISSN 0013-8703 Institutional support: RVO:61388963 Keywords : Hymenoptera * Apidae * Psithyrus * social parasitism * repellent * GC-EAD * chemical camouflage Subject RIV: EG - Zoology Impact factor: 1.442, year: 2015

  8. New Records of Bee Genera (Hymenoptera: Apoidea from Colombia

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    Víctor H. González

    2006-07-01

    Full Text Available The solitary bee genera Lophothygater Moure y Michener (Apidae, Eucerini and Tapinotaspoides Moure (Apidae, Tapinotaspidini are reported from northern Colombia for the first time. These genera were previously known from the central Brazilian Amazonian and Argentina and Paraguay, respectively.

  9. Author Details

    African Journals Online (AJOL)

    Improving honey production in worker bees (Apis mellifera adansoni L.) hymenoptera: apidae through artificial modification of their feeding activities. Abstract PDF · Vol 13, No 2 (2013) - Articles Challenges associated with the honey bee (Apis Mellifera Adansonii) colonies establishment in South Western Nigeria

  10. Density and Distribution of Xylocopa Nests (Hymenoptera: Apidae) in Caatinga Areas in the Surroundings of Passion Fruit Crops.

    Science.gov (United States)

    Martins, C F; de Siqueira, K M M; Kiill, L H P; Sá, I I S; Aguiar, C M L

    2014-08-01

    Due to their importance as pollinators of many plant species, this study aimed to know the nest density, spatial distribution, and nesting substrates used by Xylocopa species in the Caatinga, a xerophilous vegetation of Northeastern Brazil. Three areas of Caatinga in the surroundings of passion fruit crops were sampled. The bee species found in these areas were Xylocopa grisescens Lepeletier and Xylocopa frontalis (Olivier). All nests were in Commiphora leptophloeos (Burseraceae) trees (n = 113). Phytosociological analysis showed that this tree species presented the highest absolute density (212.5 individuals/ha) and index of importance value (52.7). The distribution pattern of the C. leptophloeos was aggregated. The nests were located in dead and dried branches with an average diameter of 5.3 ± 2.0 cm (n = 43). The mean number of nests/tree was 3.1 ± 2.8 (n = 113). The less disturbed area showed 6.7 nests/ha and 4.2 nests/tree. In the disturbed areas, 0.9 nests/ha and 2.4 to 2.7 nests/tree were observed. The availability of substrate for nesting in the studied areas and its importance as a limiting factor for nesting are discussed.

  11. International Journal of Biological and Chemical Sciences - Vol 11 ...

    African Journals Online (AJOL)

    ... L. 1758: Hymenoptera, Apidae) in three beekeeping sites of the Democratic Republic of Congo · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Boniface Posho Ndola, Paul Malumba, Bernard Wathelet, Eric Haubruge, Frederic Francis, Bach Kim Nguyen, 541-555.

  12. Efecto del cambio del paisaje en la estructura de la comunidad de abejas sin aguijón (Hymenoptera: Apidae en Meta, Colombia

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    Guiomar Nates-Parra

    2008-09-01

    Full Text Available Las abejas sin aguijón son unos de los polinizadores naturales más diversos de la fauna Apoidea en los trópicos. Nidifican en diversos sustratos y distintos ambientes, existiendo algunas especies típicas de lugares naturales o artificiales. La alteración del medio donde nidifican tiene un importante impacto sobre la composición natural de su estructura, hecho que se ve reflejado también en la densidad de nidos. Analizamos la composición de la estructura de la comunidad de abejas sin aguijón en tres paisajes del piedemonte llanero colombiano, una región importante por representar la transición entre ambientes andinos y de sabana (seriamente amenazada por la ganadería intensiva. Realizamos muestreos sistemáticos en bosque secundario, agro-ecosistema y zona urbana; registramos la presencia de 204 nidos de 11 géneros representados por 24 especies. La riqueza de abejas sin aguijón fue similar, aun cuando hubo diferencias significativas en la estructura de la comunidad en los tres paisajes. La densidad de nidos por paisaje fue heterogénea y nunca mayor de 16 nidos/Ha. Observamos dos patrones de nidificación y registramos diferente número de especies en la misma zona utilizando otro criterio de muestreo.Effect of landscape change on the structure of the sting-less bee community (Hymenoptera: Apidae in Meta, Colombia. Stingless bees represent one of the most diversified components of the natural Apoidea fauna of pollinators in the tropics. They use diverse kinds of substrates and inhabit varied habitats. Some species are typical for some natural either artificial place. The landscape alteration were this group of bees nests, has and important impact on the natural composition of its community structure, fact which is reflected in the nest density. We analyzed the structure composition of the stingless bees’ community in three environments in the Colombian llanos piedmont, an important region that represents the transition between

  13. Author Details

    African Journals Online (AJOL)

    Tope, SF. Vol 4, No 4 (2010) - Articles Foraging behaviour of Apis mellifera adansonii Latreille (Hymenoptera : Apidae) on Daniellia oliveri, Delonix regia, Hymenocardia acida and Terminalia mantaly flowers in Ngaoundéré (Cameroon) Abstract PDF. ISSN: 1997-342X. AJOL African Journals Online. HOW TO USE AJOL.

  14. Author Details

    African Journals Online (AJOL)

    Mbianda, AP. Vol 4, No 4 (2010) - Articles Foraging behaviour of Apis mellifera adansonii Latreille (Hymenoptera : Apidae) on Daniellia oliveri, Delonix regia, Hymenocardia acida and Terminalia mantaly flowers in Ngaoundéré (Cameroon) Abstract PDF. ISSN: 1997-342X. AJOL African Journals Online. HOW TO USE AJOL ...

  15. Inbreeding and building up small populations of stingless bees (Hymenoptera, Apidae

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    Paulo Nogueira-Neto

    2002-12-01

    Full Text Available A study of the viability of small populations of Hymenoptera is a matter of importance to gain a better zoological, ethological, genetical and ecological knowledge of these insects, and for conservation purposes, mainly because of the consequences to the survival of colonies of many species of bees, wasps, and ants. Based on the Whiting (1943 principle, Kerr & Vencovski (1982 presented a hypothesis that states that viable populations of stingless bees (Meliponini should have at least 40 colonies to survive. This number was later extended to 44 colonies by Kerr (1985. This would be necessary to avoid any substantial amount of homozygosis in the pair of chromosomic sexual loci, by keeping at least six different sexual gene alleles in a reproductive population. In most cases this would prevent the production of useless diploid males. However, several facts weigh against considering this as a general rule. From 1990 to 2001, 287 colony divisions were made, starting with 28 foundation colonies, in the inbreeding and population experiments with the Meliponini reported here. These experiments constitute the most extensive and longest scientific research ever made with Meliponini bees. In ten different experiments presented here, seven species (one with two subspecies of Meliponini bees were inbred in five localities inside their wide-reaching native habitats, and in two localities far away from these habitats. This was done for several years. On the whole, the number of colonies increased and the loss of colonies over the years was small. In two of these experiments, although these populations were far (1,000 km and 1,200 km from their native habitat, their foundation colonies were multiplied successfuly. It was possible to build up seven strong and three expanding medium populations, starting with one, two, three or even five colonies. However, in six other cases examined here, the Whiting (1943 principle and the hypothesis of Kerr & Vencovski (1982

  16. Pollination and yield responses of cowpea ( Vigna unguiculata L ...

    African Journals Online (AJOL)

    To determine the apicultural value of Vigna unguiculata (L.) Walp. (Fabaceae) and evaluate the Apis mellifera adansonii Latreille (Hymenoptera: Apidae) activity on its pod and seed yields, the bee foraging and pollinating activities were studied in Ngaoundéré. The experiment was carried out within the University of ...

  17. Author Details

    African Journals Online (AJOL)

    Fasasi, KA. Vol 13, No 1 (2011) - Articles Studies on the life cycle and morphometrics of honeybees, Apis Mellifera adansonii (Hymenoptera: Apidae) in a Mangrove Area of Lagos, Nigeria Abstract PDF. ISSN: 0794-4896. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors ...

  18. Browse Title Index

    African Journals Online (AJOL)

    Items 351 - 400 of 900 ... OC Odoli, PM Oduor-Odote, B Ohowa, SO Onyango. Vol 15, No 2 (2015), Evaluation of four apicultural products for hive colonization by honey bees (Apis mellifera adansonii) (Hymenoptera: apidae) and pre-colonization pests, Abstract PDF. SA Babarinde, SA Olaleke, AO Akinyemi, TA Adebayo, ...

  19. Following the cold: geographical differentiation between interglacial refugia and speciation in the arcto-alpine species complex Bombus monticola (Hymenoptera: Apidae)

    Czech Academy of Sciences Publication Activity Database

    Martinet, B.; Lecocq, T.; Brasero, N.; Biella, Paolo; Urbanová, Klára; Valterová, Irena; Cornalba, M.; Gjershaugh, J. O.; Michez, D.; Rasmont, P.

    2018-01-01

    Roč. 43, č. 1 (2018), s. 200-217 ISSN 0307-6970 R&D Projects: GA ČR(CZ) GP14-10035P Grant - others:GA JU(CZ) 152/2016/P Institutional support: RVO:60077344 ; RVO:61388963 Keywords : Bombus monticola * geographical differentiation * Hymenoptera Subject RIV: EH - Ecology, Behaviour; CB - Analytical Chemistry, Separation (UOCHB-X) OBOR OECD: Ecology; Analytical chemistry (UOCHB-X) Impact factor: 4.474, year: 2016 http://onlinelibrary.wiley.com/doi/10.1111/syen.12268/full

  20. Termorregulação colonial e a influência da temperatura no desenvolvimento da cria em abelhas sem ferrão, Melipona scutellaris (Hymenoptera, Apidae, Meliponini)

    OpenAIRE

    Yara Sbrolin Roldão

    2011-01-01

    Uma característica dos insetos sociais, entre elas as abelhas, é o controle da temperatura de seus ninhos. Abelhas sociais são denominadas como animais heterotérmicos, ou seja, são endotérmicas quando realizam atividades motoras (adultas) e ectotérmicas quando apresentam inatividade (cria e abelhas jovens). Essa característica está entrelaçada com o comportamento social. As abelhas melíferas (Apidae; Apini: Apis mellifera) são conhecidas por apresentarem uma temperatura ótima dentro de seus n...

  1. Déficit de polinização da aceroleira no período seco no semiárido paraibano

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    Rozileudo da Silva Guedes

    2011-06-01

    Full Text Available A aceroleira é uma importante frutífera tropical que pode produzir frutos o ano todo na região semiárida do Nordeste do Brasil, caso seja utilizada a irrigação. Como a aceroleira depende da polinização por abelhas coletoras de óleos florais para apresentar uma produção satisfatória de frutos, este trabalho teve como objetivo avaliar a abundância de polinizadores e o sucesso reprodutivo da cultura no período seco, no semiárido paraibano. Foram registradas quatro espécies de abelhas nativas da tribo Centridini, todas consideradas polinizadoras efetivas da aceroleira, pela frequência nas flores e comportamento: Centris aenea Lepeletier, C. tarsata Smith, C. fuscata Lepeletier e C. trigonoides Lepeletier (Apidae, Centridini. A frequência de visitas às flores foi menor no período seco do que no período chuvoso. A polinização cruzada manual complementar resultou em incremento de 61 a 74% na produção de frutos durante o período seco, nos dois anos avaliados, indicando que há um grande déficit de polinização devido à baixa abundância de abelhas Centris. Esse resultado implica a necessidade de manejo dos polinizadores, especialmente em cultivos irrigados durante o período seco, na região semiárida do Nordeste do Brasil.

  2. Eumelanin and pheomelanin are predominant pigments in bumblebee (Apidae: Bombus pubescence

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    Carlo Polidori

    2017-05-01

    Full Text Available Background Bumblebees (Hymenoptera: Apidae: Bombus are well known for their important inter- and intra-specific variation in hair (or pubescence color patterns, but the chemical nature of the pigments associated with these patterns is not fully understood. For example, though melanization is believed to provide darker colors, it still unknown which types of melanin are responsible for each color, and no conclusive data are available for the lighter colors, including white. Methods By using dispersive Raman spectroscopy analysis on 12 species/subspecies of bumblebees from seven subgenera, we tested the hypothesis that eumelanin and pheomelanin, the two main melanin types occurring in animals, are largely responsible for bumblebee pubescence coloration. Results Eumelanin and pheomelanin occur in bumblebee pubescence. Black pigmentation is due to prevalent eumelanin, with visible signals of additional pheomelanin, while the yellow, orange, red and brown hairs clearly include pheomelanin. On the other hand, white hairs reward very weak Raman signals, suggesting that they are depigmented. Additional non-melanic pigments in yellow hair cannot be excluded but need other techniques to be detected. Raman spectra were more similar across similarly colored hairs, with no apparent effect of phylogeny and both melanin types appeared to be already used at the beginning of bumblebee radiation. Discussion We suggest that the two main melanin forms, at variable amounts and/or vibrational states, are sufficient in giving almost the whole color range of bumblebee pubescence, allowing these insects to use a single precursor instead of synthesizing a variety of chemically different pigments. This would agree with commonly seen color interchanges between body segments across Bombus species.

  3. Interactions between carpenter bees and orchid bees (Hymenoptera: Apidae in flowers of Bertholletia excelsa Bonpl. (Lecythidaceae Interações entre abelhas carpinteiras e abelhas das orquídeas (Hymenoptera: Apidae em flores de Bertholletia excelsa Bonpl. (Lecythidaceae

    Directory of Open Access Journals (Sweden)

    Charles Fernando dos Santos

    2012-03-01

    Full Text Available Competition between two species of bees for the same type of floral resource may generate antagonistic behavior between them, especially in cultivated areas where food resources are limited, seasonally and locally. In this study, was tested the hypothesis of antagonism between two solitary bee species of the family Apidae, Eulaema mocsaryi (Euglossini and Xylocopa frontalis (Xylocopini, visiting the Brazil nut flowers (Bertholletia excelsa: Lecythidaceae in a central Amazonia agricultural area. The visitation time was analyzed to detect the possible temporal overlap in the foraging of these bees. Furthermore, was analyzed their interspecific interactions for manipulating flower species visited by an opponent species, as well as attempts to attack this opponent. The individuals of Xylocopa frontalis visited the Brazil nut flowers before Eulaema mocsaryi, although the peak visitation of both did not presented significant differences. Neither of the species manipulated flowers recently visited by opponent species, and there were practically no antagonistic interactions between them. Thus, X. frontalis and E. mocsaryi shared the same food source in the flowers of B. excelsa due to differences in their time of visits and non-aggressive way of interacting with the opponent. This result has important implications for pollinating the Brazil nut, and a possible management of X. frontalis and E. mocsaryi, since these two were the most abundant pollinators in the studied locality.A competição entre duas espécies de abelhas por um mesmo tipo de recurso floral pode gerar comportamentos antagônicos entre elas, principalmente, dentro de áreas cultivadas, onde o recurso alimentar é limitado sazonalmente e localmente. No presente trabalho, foi testada a hipótese de antagonismo entre duas espécies de abelhas solitárias da família Apidae, Eulaema mocsaryi (Euglossini e Xylocopa frontalis (Xylocopini em flores da castanheira do Brasil (Bertholletia

  4. Author Details

    African Journals Online (AJOL)

    Fasasi, KA. Vol 8 (2010) - Articles Effect of age of honeybee queens of Apis mellifera adansonii (Hymenoptera: apidae) on the rate of brood production in modern bee-hives. Abstract. ISSN: 1596-972X. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about ...

  5. Biologia floral de Croton urucurana Baill. (Euphorbiaceae ocorrente em vegetação ripária da ilha Porto Rico, Porto Rico, Estado do Paraná, Brasil - DOI: 10.4025/actascibiolsci.v26i2.1638 Floral biology of Croton urucurana Baill (Euphorbiaceae that occurs in riparian vegetation of Porto Rico Island, in Porto Rico, Paraná, Brazil - DOI: 10.4025/actascibiolsci.v26i2.1638

    Directory of Open Access Journals (Sweden)

    Luiz Antonio de Souza

    2004-04-01

    Full Text Available Foram realizados estudos de biologia floral em Croton urucurana, espécie monóica, arbórea, pioneira e recomendada para a recuperação de matas ciliares. As flores são diclinas, pequenas, apresentam coloração amarelo-esverdeada e dispõem-se em inflorescências racemosas. A antese é noturna, por volta das 23h, e as flores duram três dias. Os visitantes florais foram insetos das ordens Diptera, Odonata, Lepidoptera, Hemiptera e Hymenoptera (Apidae e Vespidae, sendo Apis mellifera a espécie mais freqüenteStudies of floral biology were developed on Croton urucurana, a pioneer monoecious tree, recommended to be used in the process of regeneration of the riparian forest. The plant presents small yellow-greenish unisexual flowers, disposed in racemose inflorescences. Anthesis occurs at night, flowers last three days and open around 11 pm. Flowers are visited by insects belonging to the following Orders Diptera, Odonata, Lepidoptera, Hemiptera and Hymenoptera (Apidae and Vespidae. Apis mellifera was the most frequent flower visitor

  6. Ecotoxicological effects of the insecticide fipronil in Brazilian native stingless bees Melipona scutellaris (Apidae: Meliponini).

    Science.gov (United States)

    de Morais, Cássio Resende; Travençolo, Bruno Augusto Nassif; Carvalho, Stephan Malfitano; Beletti, Marcelo Emílio; Vieira Santos, Vanessa Santana; Campos, Carlos Fernando; de Campos Júnior, Edimar Olegário; Pereira, Boscolli Barbosa; Carvalho Naves, Maria Paula; de Rezende, Alexandre Azenha Alves; Spanó, Mário Antônio; Vieira, Carlos Ueira; Bonetti, Ana Maria

    2018-09-01

    Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae) is a pollinator of various native and cultivated plants. Because of the expansion of agriculture and the need to ensure pest control, the use of insecticides such as fipronil (FP) has increased. This study aimed to evaluate the effects of sublethal doses of FP insecticide on M. scutellaris at different time intervals (6, 12, and 24 h) after exposure, via individually analyzed behavioral biomarkers (locomotor activity, behavioral change) as well as the effect of FP on different brain structures of bees (mushroom bodies, antennal cells, and optic cells), using sub-individual cell biomarkers (heterochromatin dispersion, total nuclear and heterochromatic volume). Forager bees were collected when they were returning to the nest and were exposed to three different concentrations of FP (0.40, 0.040, and 0.0040 ng a.i/bee) by topical application. The results revealed a reduction in the mean velocity, lethargy, motor difficulty, paralysis, and hyperexcitation in all groups of bees treated with FP. A modification of the heterochromatic dispersion pattern and changes in the total volume of the nucleus and heterochromatin were also observed in the mushroom bodies (6, 12, and 24 h of exposure) and antennal lobes (6 and 12 h) of bees exposed to 0.0040 ng a.i/bee (LD 50/100 ). FP is toxic to M. scutellaris and impairs the essential functions required for the foraging activity. Copyright © 2018 Elsevier Ltd. All rights reserved.

  7. Refugia, biodiversity, and pollination roles of bumble bees in the Madrean Archipelago

    Science.gov (United States)

    Justin O. Schmidt; Robert S. Jacobson

    2005-01-01

    Eight species of bumble bees (Hymenoptera: Apidae: Bombus) are present within five major Sky Island mountains of southern Arizona. Another four species exist in the nearby large mountainous region stretching from the Arizona White Mountains to Flagstaff. The distribution and number of bumble bee species within the individual Sky Island mountains varies from six in the...

  8. Variación de la comunidad de abejas de las orquídeas (Hymenoptera: Apidae en tres ambientes perturbados del piedemonte llanero colombiano

    Directory of Open Access Journals (Sweden)

    Alejandro Parra-H

    2007-12-01

    Full Text Available Las abejas de las orquídeas subsisten en vastas áreas de bosque tropicales gracias a que mantienen estrechas relaciones con tipos de vegetación particular en diversos micro-hábitats. Con base en este tipo de relaciones con el medio y características biológicas como preferencia por ciertos tipos de néctares y de polen, y diversidad morfológica y etológica de la tribu, es posible evaluar la calidad de un hábitat según la distribución de euglosinos. Este trabajo propone el uso de esta información, además de índices de diversidad para la evaluación de la calidad del medio. Entre marzo y diciembre de 2003 muestreamos tres tipos de paisaje (Urbano, Rural y Conservado en el piedemonte llanero colombiano, usando redes entomológicas y sustancias aromáticas (Cineol y Metil Salicilato. Para las 15 localidades muestreadas se registraron 17 de las 26 especies conocidas para el área. Eulaema nigrita fue la más frecuente mientras que Euglossa magnipes, E. cybelia, E. heterosticta, E. singularis, Eulaema bombiformis, E. speciosa y Exaerete frontalis correspondieron a ambientes considerados de aceptable a buena calidad. La composición y cercanía de fragmentos de bosque son factores favorables. La diversidad relativa (máxima variación de formas y tamaños dentro de la tribu, sería proporcional a la calidad del medio.Variation of the orchid bees community (Hymenoptera: Apidae in three altered habitats of the Colombian "llano" piedmont. Orchid bees subsist in vast tropical forest areas because they maintain close relationships with particular plant species in diverse micro-habitats. Based on the relationships among the environment and biological features (food preference, morphologic and ethologic diversity, it is possible to determine habitat quality using the euglossine array. This work proposes the use of this ecological information, in addition to diversity indices, for the evaluation of environmental quality. Fifteen localities in

  9. CORRELAÇÃO ENTRE PARÂMETROS BIOMÉTRICOS E PRODUTIVOS EM COLÔNIAS DE Melipona quadrifasciata anthidioides LEPELETIER (HYMENOPTERA: APIDAE

    Directory of Open Access Journals (Sweden)

    Patricia Faquinello

    2013-09-01

    Full Text Available In meliponini colonies, biometric characteristics may be associated with production traits, thus the study of correlations is extremely useful as a tool for colony selection process. The aim of this study was to estimate the correlations between biometric and productive parameters of Melipona quadrifasciata anthidioides. We analyzed 128 colonies, from 60 parental and two generations, F1 and F2. The following parameters were evaluated: queen and colony weight; number, length and width of brood disks; number, width, depth and volume of honey pots; number, width and depth of pollen pots; glossa size, and estimate of the population and honey production. The queen weight was correlated with the number of brood disks (0.23 and population (0.23, as well as the characteristic number of pollen pots is related to the length and width of brood disks and population (0.88, 0.54 and 0.52, respectively. The characteristic honey production is related to the number (0.93, width (0.50 and volume (0.47 of honey pots. The results showed that honey production is directly correlated with number, volume, width and depth of honey pots. On the other side, the population size was correlated with the number of brood disks and pollen pots.

  10. Cytogenetic characterization of Melipona rufiventris Lepeletier 1836 and Melipona mondury Smith 1863 (Hymenoptera, Apidae by C banding and fluorochromes staining

    Directory of Open Access Journals (Sweden)

    Denilce Meneses Lopes

    2008-01-01

    Full Text Available The stingless bees Melipona rufiventris and M. mondury were analyzed cytogenetically by conventional staining with Giemsa, C-banding and sequential staining with the fluorochromes CMA3/DA/DAPI. Both species presented 2n = 18 and n = 9, except for one colony of M. rufiventris, in which some individuals had 2n = 19 due to the presence of a B chromosome. After Giemsa staining and C-banding the chromosomes appeared very condensed and presented a high heterochromatic content, making it difficult to localize the centromere and therefore to visualize the chromosomes morphology. The constitutive heterochromatin was located in interstitial chromosome regions covering most of the chromosomes extension and consisted mainly of AT, as shown by DAPI staining. The euchromatin was restricted to the chromosome extremities and was GC-rich, as evidenced by CMA3 staining. The B chromosome was CMA3-negative and DAPI-positive, a heterochromatic constitution similar to that of the A genome chromosomes.

  11. Floral preferences and climate influence in nectar and pollen foraging by Melipona rufiventris Lepeletier (Hymenoptera: Meliponini) in Ubatuba, São Paulo state, Brazil.

    Science.gov (United States)

    Fidalgo, Adriana de O; Kleinert, Astrid de M P

    2010-01-01

    We describe the environment effects on the amount and quality of resources collected by Melipona rufiventris Lepeletier in the Atlantic Forest at Ubatuba city, São Paulo state, Brazil (44º48'W, 23º22'S). Bees carrying pollen and/or nectar were captured at nest entrances during 5 min every hour, from sunrise to sunset, once a month. Pollen loads were counted and saved for acetolysis. Nectar was collected, the volume was determined and the total dissolved solids were determined by refractometer. Air temperature, relative humidity and light intensity were also registered. The number of pollen loads reached its maximum value between 70% and 90% of relative humidity and 18ºC and 23ºC; for nectar loads this range was broader, 50-90% and 20-30ºC. The number of pollen loads increased as relative humidity rose (rs = 0.401; P < 0.01) and high temperatures had a strong negative influence on the number of pollen loads collected (rs = -0.228; P < 0.01). The number of nectar loads positively correlated with temperature (rs = 0.244; P < 0.01) and light intensity (rs = 0.414; P < 0.01). The percentage of total dissolved solids (TDS) on nectar loads positively correlated with temperature and light intensity (rs = 0.361; P < 0.01 and rs = 0.245; P < 0.01), negatively correlated with relative humidity (rs = -0.629; P < 0.01), and it increased along the day. Most nectar loads had TDS between 11% and 30%, with an average of 24.7%. The volume measures did not show any pattern. Important pollen sources were Sapindaceae, Anacardiaceae, Rubiaceae, Arecaceae, Solanaceae and Myrtaceae; nectar sources were Sapindaceae, Fabaceae, Rubiaceae, Arecaceae and Solanaceae.

  12. Subfamily Limoniinae Speiser, 1909 (Diptera, Limoniidae) from Baltic amber (Eocene): the genus Helius Lepeletier & Serville, 1828.

    Science.gov (United States)

    Kania, Iwona

    2014-06-10

    A revision of the genus Helius Lepeletier & Serville, 1828 (Diptera: Limoniidae) from Baltic amber (Eocene) is presented. Redescriptions of 5 species, Helius formosus Krzemiński, 1993, Helius linus Podenas, 2002, Helius minutus (Loew, 1850), Helius mutus Podenas, 2002, Helius pulcher (Loew, 1850) of this genus from Baltic amber are given and documented by photographs and drawings. Four new species of the genus Helius from Baltic amber are described: Helius gedanicus sp. nov., Helius hoffeinsorum sp. nov., Helius similis sp. nov., Helius fossilis sp. nov. A key to species of Helius from Baltic amber is provided. Patterns morphological evolution and the aspects evolutionary history of Helius are discussed.

  13. Digestive and regenerative cells in the midgut of haploid and diploid males of the stingless bee Melipona quadrifasciata anthidioides (Hymenoptera: Apidae

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    Kenner M. Fernandes

    2012-10-01

    Full Text Available In eusocial bees, workers and queens are diploid (2n, whereas males are haploid (n. However, in some species, including the stingless bee Melipona quadrifasciata anthidioides Lepeletier, 1836, 2n males arise from fertilized eggs resulting from the crossing between a queen and her brother. In the present study, we provide a comparative analysis of the digestive and regenerative cells in n and 2n pupae and adult males of M. quadrifasciata anthidioides. In n and 2n pupae and adult males, the number of regenerative cells/nest was similar. In n and 2n pupae, the mean number of digestive cells/midgut area was 2076 ± 0.60, whereas in adults it was 1234 ± 1.42 digestive cells/midgut area. The nuclear area of the digestive cells was also similar in both n and 2n adult males (~154 µm² and smaller in pupae (~91 µm²; this variation might be a result of DNA amplification in digestive cells during bee development. The results from our current study provide further understanding of the morphological and physiological aspects of the digestive tract of bees and show that the ploidy difference between n and 2n male stages does not affect the number of digestive and regenerative cells in the midgut of M. quadrifasciata anthidioides.

  14. Estudo Comparativo das Sensilas Antenais de Operárias de Melipona scutellaris Latreille (Hymenoptera: Apidae de Diferentes Altitudes

    Directory of Open Access Journals (Sweden)

    Marcília Aparecida Nascimento

    2013-04-01

    Full Text Available Sensilas são estruturas sensoriais presentes nas antenas dos insetos e são responsáveis pela higro- quimio-, termo- e mecanorrecepção. No presente trabalho, as sensilas presentes nos três flagelômeros (F mais distais de Melipona scutellaris Latreille (Hymenopera: Apidae coletadas em diferentes altitudes (200 e acima de 900m foram avaliadas qualitativamente e quantitativamente. A identificação, contagem e medição das sensilas foram feitas a partir de imagens da superfície dorsal das antenas que foram obtidas com o auxílio de microscópio eletrônico de varredura. Foram observadas as sensilas placoide, basicônica, celocônica, ampulácea, campaniforme e tricoide reta e curvada. Comparativamente, o grupo localizado a 200m de altitude possui sensilas tricoides retas em maior quantidade nos três flagelômeros, enquanto que o grupo de altitudes acima de 900m apresentou maior quantidade de sensilas tricoides curvadas no F9, sendo as mesmas maiores para o F10. Essas diferenças foram discutidas em função da localização geográfica dos diferentes grupos aqui estudados. Os resultados da investigação da estrutura antenal de M. scutellaris contribuem para um melhor entendimento da biologia dessa espécie.

  15. Morfologia externa da operária de Lestrimelitta ehrhardtti (Hymenoptera: Meliponini

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    DANIELE. R PARIZOTTO

    2010-05-01

    Full Text Available ABSTRACT  Workers of Lestrimelitta ehrhardtti Friese were studied based on a great number of individuals collected in Castro municipality, Paraná State, Brazil. The specimens were described morphologically, measured and illustrated. KEY-WORDS: Apidae, stingless-bees, cleptobiotic, Neotropical.   RESUMEN Se estudió un gran número de obreras de Lestrimelitta ehrhardtti Friese colectadas en el municipio de Castro, Paraná, Brasil. Los especímenes fueran descritos  morfológicamente, medidos e ilustrados. PALABRAS CLAVE: Apidae, abejas sin aguijón, cleptobiótica, Neotropical.

  16. Registro de Nephridiophaga sp. (Protista: Nephridiophagidae en Apis mellifera (Hymenoptera: Apidae del Sur de la región Pampeana Record of Nephridiophaga sp. (Protista: Nephridiophagidae in Apis mellifera (Hymenoptera: Apidae of the southern Pampas

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    Santiago Plischuk

    2011-12-01

    Full Text Available Durante estudios prospectivos tendientes a la detección de protistas asociados a ápidos en la región Pampeana, se observó la presencia de esporos ovales bicóncavos y grupos de esporos (cúmulos en los túbulos de Malpighi de abejas de Dufaur, partido de Saavedra, sudoeste de la provincia de Buenos Aires. Los esporos maduros midieron 4,8 ± 0,05 x 2,4 ± 0,03 μm y la carga (intensidad promedió 5,71 ± 1,49 x 10(6 esporos/abeja. Las detecciones se efectuaron entre julio y octubre de 2006 y la prevalencia en las colmenas positivas osciló entre 1 y 16,7 %. Las características morfológicas de los esporos, el lugar de desarrollo y la especie huésped involucrada sugieren que el microorganismo en cuestión, pertenece al género Nephridiophaga y sería N. apis Ivani, especie tipo cuyo conocimiento es extremadamente limitado. El hallazgo constituye el primer registro de un nefridiofágido asociado a A mellifera fuera del continente europeo.During surveys for the detection of protists associated to Apidae in the Pampas region, biconcave oval spores, and spore clumps were observed in the Malpighian tubules of honeybees from Dufaur, Saavedra county, southwestern Buenos Aires province. Mature spores measured 4.8 ± 0.05 x 2.4 ± 0.03 μm, and mean spore load was 5.71 ± 1.49 x 10(6 per honeybee. Detections were from July to October 2006, and prevalence in positive colonies ranged from 1 to 16.7%. Morphology of the spores, the site of development, and the identity of the host species suggest that the isolated microorganism belongs to the genus Nephridiophaga and would be N apis Ivani, the type species, knowledge on which is extremely limited. The finding constitutes the first record of a nephridiophagid in honeybees outside of Europe.

  17. HYMENOPTERA ALLERGENS: FROM VENOM TO VENOME

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    Edzard eSpillner

    2014-02-01

    Full Text Available In Western Europe hymenoptera venom allergy primarily relates to venoms of the honeybee and the common yellow jacket. In contrast to other allergen sources, only a few major components of hymenoptera venoms had been characterized until recently. Improved expression systems and proteomic detection strategies have allowed the identification and characterization of a wide range of additional allergens. The field of hymenoptera venom allergy research has moved rapidly from focusing on venom extract and single major allergens to a molecular understanding of the entire venome as a system of unique and characteristic components. An increasing number of such components has been identified, characterized regarding function and assessed for allergenic potential. Moreover, advanced expression strategies for recombinant production of venom allergens allow selective modification of molecules and provide insight into different types of IgE reactivities and sensitization patterns. The obtained information contributes to an increased diagnostic precision in hymenoptera venom allergy and may serve for monitoring, reevaluation and improvement of current therapeutic strategies.

  18. The Activity of Carbohydrate-Degrading Enzymes in the Development of Brood and Newly Emerged workers and Drones of the Carniolan Honeybee, Apis mellifera carnica

    OpenAIRE

    Żółtowska, Krystyna; Lipiński, Zbigniew; Łopieńska-Biernat, Elżbieta; Farjan, Marek; Dmitryjuk, Małgorzata

    2012-01-01

    The activity of glycogen Phosphorylase and carbohydrate hydrolyzing enzymes α-amylase, glucoamylase, trehalase, and sucrase was studied in the development of the Carniolan honey bee, Apis mellifera carnica Pollman (Hymenoptera: Apidae), from newly hatched larva to freshly emerged imago of worker and drone. Phosphorolytic degradation of glycogen was significantly stronger than hydrolytic degradation in all developmental stages. Developmental profiles of hydrolase activity were similar in both ...

  19. Hymenoptera stings in Brazil: a neglected health threat in Amazonas State

    OpenAIRE

    Costa, Allyson Guimarães; Chaves, Bárbara Aparecida; Murta, Felipe Leão Gomes; Sachett, Jacqueline Almeida Gonçalves; Sampaio, Vanderson Souza; Silva, Vanessa Costa; Monteiro, Wuelton Marcelo

    2018-01-01

    Abstract INTRODUCTION: Hymenoptera injuries are commonly caused by stinging insects. In Amazonas state, Brazil, there is no information regarding distribution, profile, and systemic manifestations associated with Hymenoptera injuries. METHODS: This study aimed to identify risk factors for systemic manifestation using the Brazilian Notifiable Diseases Surveillance System (2007 to 2015). RESULTS: Half of Hymenoptera injuries were caused by bee stings. Hymenoptera injuries were concentrate...

  20. Melittofauna and Other Potential Pollinators in Wetland and Uplands in South Central Nebraska (Insecta: Apoidea).

    Science.gov (United States)

    Park, Cynthia N; Overall, Lisa M; Smith, Loren M; Lagrange, Ted; McMurry, Scott

    2017-03-10

    Our objective was to document potential wild pollinating insects in south central Nebraska. This intensively cultivated region is known as the Rainwater Basin and contains some of the most endangered wetland systems in North America. We used blue vane traps to passively collect insects and insect nets to actively collect on flowering plants from April through October in 2014 and 2015. Habitat types included playa wetlands, adjacent mixed and tallgrass prairies, and agricultural fields. Over 112,000 insects were collected; Hymenoptera represented 78% of the total, and the families Apidae and Halictidae comprised 99% of the total melittofauna. Insects from 13 orders were collected, but Hymenoptera, Diptera, and Coleoptera were the most abundant potential pollinators.

  1. Isolation of biologically active peptides from the venom of Japanese carpenter bee, Xylocopa appendiculata

    OpenAIRE

    Kawakami, Hiroko; Goto, Shin G.; Murata, Kazuya; Matsuda, Hideaki; Shigeri, Yasushi; Imura, Tomohiro; Inagaki, Hidetoshi; Shinada, Tetsuro

    2017-01-01

    Background Mass spectrometry-guided venom peptide profiling is a powerful tool to explore novel substances from venomous animals in a highly sensitive manner. In this study, this peptide profiling approach is successfully applied to explore the venom peptides of a Japanese solitary carpenter bee, Xylocopa appendiculata (Hymenoptera: Apoidea: Apidae: Anthophila: Xylocopinae: Xylocopini). Although interesting biological effects of the crude venom of carpenter bees have been reported, the struct...

  2. Biologia de Centris Fabricius (Hymenoptera, Anthophoridae, Centridini em matas contínuas e fragmentos na Amazônia Central Biology of Centris Fabricius (Hymenoptera, Anthophoridae, Centridini in continuous forest and fragments in Central Amazonia

    Directory of Open Access Journals (Sweden)

    Elder F. Morato

    1999-01-01

    Full Text Available A study of four tree-hole nesting bee species of Centris Fabricius, 1804 was conducted between July, 1988 and June, 1990 in isolated and continuous "terra firme" forests at Manaus region. Nests were obtained from wood trap-nests of different diameters and consisted of a linear series of brood cells. Centris dichrootricha (Moure, 1945 and C. terminata Smith, 1874 were the most abundant species. Centris terminata nested principally in gaps and continuous forest and C. dichrootricha in cleared areas and small fragments of forest. More nests were obtained in trap-nests placed at 8 and 15 m height above ground. Nesting activity was more pronounced in the wet season between August and January. Natural enemies are the beetle Tetraonyx Latreille, 1805 and the bees Coelioxys Latreille, 1809, Mesocheira Lepeletier & Serville, 1825 and Mesoplia Lepeletier, 1841. Nest architecture of Centris spp. are described.

  3. Hymenoptera: Formicidae

    African Journals Online (AJOL)

    1990-05-15

    May 15, 1990 ... Supplemental Library User's Guide 1983 Edition. pp. 131-144. (Ed.) S. Ioyner. SAS Institute Incorporated, Cary,. North Carolina. CORBARA, B., LACHAUD, I. & FRESNEAU, D. 1989. Individual variability, social structure and division of labour in the ponerine ant, Ectatommo. ruidum Roger (Hymenoptera,.

  4. [Bee diversity in Tecoma stans (L.) Kunth (Bignoniaceae): importance for pollination and fruit production].

    Science.gov (United States)

    Silva, Cláudia I; Augusto, Solange C; Sofia, Silvia H; Moscheta, Ismar S

    2007-01-01

    Tecoma stans (L.) Kunth is an exotic plant in Brazil, commonly distributed in urban areas, which is considered an invasive species in crop and pasture areas. In this study, the floral biology and the behavior of bees in flowers of T. stans from three urban areas in southeastern Brazil were investigated. In all study sites, T. stans was an important food resource to the Apoidea to 48 species of bees. Centris tarsata Smith and Exomalopsis fulvofasciata Smith (Hymenoptera: Apidae) were the effective pollinators more abundant, while Scaptotrigona depilis Moure (Hymenoptera: Apidae) was the more frequent robber species. The most part of T. stans visitors (87.5%) exploited exclusively nectar, which varied in sugar concentration depending on the day period and flower phase. In all flower stages, higher averages of nectar concentration (26.4% to 32.7%) occurred from 10 am to 2 pm. The presence of osmophore in the petals and protandry were detected. In two urban areas the number of visitors varied significantly during the day. The greatest abundance of pollinators occurred when pollen availability was higher and flowers showed receptive stigma, which could be contributing to the reproductive success of T. stans. The results indicate that the production of fruits increased in plants that received a higher number of effective pollinators.

  5. Abelhas (Hymenoptera: Apoidea visitantes das flores de urucum em Vitória da Conquista, BA Bees (Hymenoptera: Apoidea visitors of the annatto flowers in Vitória da Conquista, Bahia State, Brazil

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    Augusto Jorge Cavalcante Costa

    2008-04-01

    Full Text Available O urucum é um arbusto da família Bixaceae, utilizado na fabricação de corantes naturais para a indústria alimentícia e cosmética. No Brasil, somente nos últimos 15 anos, houve maior interesse pelo cultivo, pois se tornou uma alternativa agrícola promissora. O presente trabalho teve por objetivo identificar as abelhas visitantes das flores do urucuzeiro em Vitória da Conquista, BA. O trabalho foi conduzido no campo experimental da UESB, em uma lavoura do tipo cultivado Peruana Paulista. A coleta das abelhas visitantes foi feita na época principal de floração do urucueiro: março/abril, das 6h às 18h. Foram coletadas 3019 abelhas de 22 espécies, com predominância na visitação das 8h às 14h em relação ao número de indivíduos e número de espécies capturadas. As espécies mais freqüentes foram: Trigona spinipes (Fabricius, Apis mellifera L., Schwarziana quadripunctata (Lepeletier e Tetragonisca angustula (Latreille. Espécies de maior porte, como Xylocopa frontalis (Olivier, Bombus morio (Swederus e Eulaema nigrita Lepeletier, consideradas como eficientes na polinização da cultura do urucum, não foram abundantes neste estudo.Annatto is a shrub from Bixaceae family, which natural pigment (annatto is widely used in food and cosmetic industries. In Brazil, the interest for this crop started in the last fifteen years, once it became a promising agricultural alternative. This study was aimed at identifing visitor bees of annatto flowers at the agriculture region of Vitória da Conquista (BA. The research was carried out in the experimental field of UESB, in an experimental plot planted with the cv. Peruana Paulista. The visitor bees were collected during the main blooming period: March/April, between 6:00h and 18:00h. A total of 3,019 bees from 22 species was collected, with higher visitation during the period from 8:00 to 14:00h, regarding the number of individuals and species. The species most frequent were Trigona spinipes

  6. Diversity of peptidic and proteinaceous toxins from social Hymenoptera venoms.

    Science.gov (United States)

    Dos Santos-Pinto, José Roberto Aparecido; Perez-Riverol, Amilcar; Lasa, Alexis Musacchio; Palma, Mario Sergio

    2018-06-15

    Among venomous animals, Hymenoptera have been suggested as a rich source of natural toxins. Due to their broad ecological diversity, venom from Hymenoptera insects (bees, wasps and ants) have evolved differentially thus widening the types and biological functions of their components. To date, insect toxinology analysis have scarcely uncovered the complex composition of bee, wasp and ant venoms which include low molecular weight compounds, highly abundant peptides and proteins, including several allergens. In Hymenoptera, these complex mixtures of toxins represent a potent arsenal of biological weapons that are used for self-defense, to repel intruders and to capture prey. Consequently, Hymenoptera venom components have a broad range of pharmacological targets and have been extensively studied, as promising sources of new drugs and biopesticides. In addition, the identification and molecular characterization of Hymenoptera venom allergens have allowed for the rational design of component-resolved diagnosis of allergy, finally improving the outcome of venom immunotherapy (VIT). Until recently, a limited number of Hymenoptera venoms had been unveiled due to the technical limitations of the approaches used to date. Nevertheless, the application of novel techniques with high dynamic range has significantly increased the number of identified peptidic and proteinaceous toxins. Considering this, the present review summarizes the current knowledge about the most representative Hymenoptera venom peptides and proteins which are under study for a better understanding of the insect-caused envenoming process and the development of new drugs and biopesticides. Copyright © 2018 Elsevier Ltd. All rights reserved.

  7. Effects of golf courses on local biodiversity.

    OpenAIRE

    Gange, A.C.; Tanner, R.A.

    2005-01-01

    There are approximately 2600 golf courses in the UK, occupying 0.7% of the total land cover. However, it is unknown whether these represent a significant resource, in terms of biodiversity conservation, or if they are significantly less diverse than the surrounding habitats. The diversity of vegetation (tree and herbaceous species) and three indicator taxa (birds, ground beetles (Coleoptera, Carabidae) and bumblebees (Hymenoptera, Apidae)) was studied on nine golf courses and nine adja...

  8. Hitting an Unintended Target: Phylogeography of Bombus brasiliensis Lepeletier, 1836 and the First New Brazilian Bumblebee Species in a Century (Hymenoptera: Apidae.

    Directory of Open Access Journals (Sweden)

    José Eustáquio Santos Júnior

    Full Text Available This work tested whether or not populations of Bombus brasiliensis isolated on mountain tops of southeastern Brazil belonged to the same species as populations widespread in lowland areas in the Atlantic coast and westward along the Paraná-river valley. Phylogeographic and population genetic analyses showed that those populations were all conspecific. However, they revealed a previously unrecognized, apparently rare, and potentially endangered species in one of the most threatened biodiversity hotspots of the World, the Brazilian Atlantic Forest. This species is described here as Bombus bahiensis sp. n., and included in a revised key for the identification of the bumblebee species known to occur in Brazil. Phylogenetic analyses based on two mtDNA markers suggest this new species to be sister to B. brasiliensis, from which its workers and queens can be easily distinguished by the lack of a yellow hair-band on the first metasomal tergum. The results presented here are consistent with the hypothesis that B. bahiensis sp. n. may have originated from an ancestral population isolated in an evergreen-forest refuge (the so-called Bahia refuge during cold, dry periods of the Pleistocene. This refuge is also known as an important area of endemism for several animal taxa, including other bees. Secondary contact between B. bahiensis and B. brasiliensis may be presently prevented by a strip of semi-deciduous forest in a climate zone characterized by relatively long dry seasons. Considering the relatively limited range of this new species and the current anthropic pressure on its environment, attention should be given to its conservation status.

  9. Ovarian egg morphology in chalcidoid wasps (Hymenoptera: Chalcidoidea parasitizing gall wasps (Hymenoptera: Cynipidae

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    Vårdal, H.

    2016-06-01

    Full Text Available We provide morphological egg data of 26 species of 5 chalcidoid families associated with cynipid galls (Hymenoptera: Cynipidae from western Palaearctic, including the first egg data for the family Ormyridae. Adult chalcidoid species were reared from galls, and eggs obtained from dissected female ovaries were examined using scanning electron microscopy (SEM. The shape of the eggs varies from oval to elongate and tapered at both ends. Eggs of Eurytomidae as well as some Eulophidae, Eupelmidae and Pteromalidae are equipped with a peduncle at the anterior end. We found a positive correlation between long eggs and long ovipositors and confirmed the expectation that eggs of endoparasitoids are generally shorter and narrower than eggs of ectoparasitoids. We were able to locate the sperm entrance or micropyle at the anterior pole of eggs of several species. It is situated at the anterior end of the egg and at the end of the peduncle when present. In addition, the eggshells of the endoparasitoid Sycophila biguttata (Swederus, 1795 (Hymenoptera: Eurytomidae and the ectoparasitoid Cecidostiba fungosa (Geoffroy, 1785 (Hymenoptera: Pteromalidae, are for the first time described.En el presente trabajo se aportan datos morfol.gicos del huevo de 26 especies del Paleártico occidental pertenecientes a 5 familias de Chalcidoidea asociadas con agallas de cinípidos (Hymenoptera: Cynipidae, incluyendo los primeros datos del huevo de especies de Ormyridae. Los ejemplares adultos de las especies estudiadas fueron obtenidos por emergencia de agallas en laboratorio, los ovarios de las hembras diseccionados para obtener los huevos, que fueron finalmente estudiados utilizando técnicas de microscopía electronica de barrido. La forma de los huevos estudiados varía de ovalada a alargada y ahusada en ambos extremos. Los huevos de Eurytomidae, así como algunos de Eulophidae, Eupelmidae y Pteromalidae están provistos de un pedúnculo en el extremo anterior. Se encontr

  10. KEANEKARAGAMAN HYMENOPTERA PARASITIKA PADA TIPE EKOSISTEM BERBEDA DI BANGKA TENGAH, KEPULAUAN BANGKA BELITUNG

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    Herry Marta Saputra

    2017-05-01

    Full Text Available Diversity of parasitic Hymenoptera in different ecosystem types in Central Bangka, Bangka-Belitung Islands. Hymenoptera richness is dominated by parasitic species. More than 80% of Hymenoptera play a role as parasitoid on arthropods that are mostly insects. Diversity of parasitic Hymenoptera is widely studied in various types of terrestrial ecosystems including agro-ecosystem and non-agro-ecosystem. This study aimed to invent and compare the diversity of parasitic Hymenoptera in three different ecosystems, i.e., forest, oil palm plantation, and ex-tin mining. The location was located in Central Bangka Regency, Bangka Island. The study was conducted in Juli 2014 until October 2015. Parasitic Hymenoptera was collected with insect sweep net and yellow pan trap on one transect line with 1000 m length. Parasitic Hymenoptera were found on forest as much as 732 morphospecies, 326 morphospecies on oil palm plantations, and 293 morphospecies on ex-tin mining. Diversity and abundance of parasitic Hymenoptera on forest was higher than oil palm plantation and ex-tin mining area. Braconidae family was found dominant on forest, however on oil palm plantation and extin mining area the dominant family was Scelionidae.

  11. Geographic distribution and spatial differentiation in the color pattern of abdominal stripes of the Neotropical stingless bee Melipona quadrifasciata (Hymenoptera: Apidae

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    Henrique Batalha-Filho

    2009-06-01

    Full Text Available Melipona quadrifasciata Lepeletier, 1836, regionally known as "mandaçaia", has been traditionally divided in two distinct subspecies: M. quadrifasciata anthidioides and M. quadrifasciata quadrifasciata. The main difference between the subspecies refers to the yellow metasomal stripes which are continuous in M. q. quadrifasciata and discontinuous in M. q. anthidioides. This study investigated the geographic differentiation in the metasomal stripes and characterized the restriction sites in the mtDNA of both chromatic types. Specimens from 198 localities were examined, and the variation observed in the pattern of stripes was grouped into distinct classes. The distribution pattern found in the present work agrees with the previously reported pattern: M. q. quadrifasciata inhabits the southern portion of the distribution, from Misiones, Argentina, southeastern Paraguay and Rio Grande do Sul to southern São Paulo, and M. q. anthidioides ranges from northeastern São Paulo to the northern Diamantina Plateau, Bahia, and westwards to the central portion of the Goiás state. It is documented for the first time the occurrence of two populations with continuous stripes inhabiting disjunct areas in relation to M. q. quadrifasciata - one in northern Minas Gerais and another in northeastern Bahia and Sergipe. The data of RFLP showed two restriction patterns, one present in M. q. quadrifasciata, and another in M. q. anthidioides and in populations with continuous metasomal stripes from northern Minas Gerais and northeastern Bahia and Sergipe. The observed patterns of geographic differentiation of M. quadrifasciata suggests the occurrence of repeated events of geographical isolation, followed by range expansion, that occurred probably during the cycles of climatic changes in the Pleistocene.

  12. Spatial Analysis of Agricultural Landscape and Hymenoptera Biodiversity at Cianjur Watershed

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    YAHERWANDI

    2006-12-01

    Full Text Available Hymenoptera is one of the four largest insect order (the other three are Coleoptera, Diptera, and Lepidoptera. There are curerently over 115 000 described Hymenoptera species. It is clear that Hymenoptera is one of the major components of insect biodiversity. However, Hymenoptera biodiversity is affected by ecology, environment, and ecosystem management. In an agricultural areas, the spatial structure, habitat diversity, and habitat composition may vary from cleared landscapes to structurally rich landscape. Thus, it is very likely that such large-scale spatial patterns (landscape effects may influence local biodiversity and ecological functions. Therefore, the objective of this research were to study diversity and configuration elements of agricultural landscapes at Cianjur Watershed with geographical information sytems (GIS and its influence on Hymenoptera biodiversity. The structural differences between agricultural landscapes of Nyalindung, Gasol, and Selajambe were characterized by patch analyst with ArcView 3.2 of digital land use data. Results indicated that class of land uses of Cianjur Watershed landscape were housing, mixed gardens, talun and rice, vegetable, and corn fields. Landscape structure influenced the biodiversity of Hymenoptera. Species richness and the species diversity were higher in Nyalindung landscape compare to Gasol and Selajambe landscape.

  13. Hymenoptera stings in Brazil: a neglected health threat in Amazonas State

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    Allyson Guimarães Costa

    Full Text Available Abstract INTRODUCTION: Hymenoptera injuries are commonly caused by stinging insects. In Amazonas state, Brazil, there is no information regarding distribution, profile, and systemic manifestations associated with Hymenoptera injuries. METHODS: This study aimed to identify risk factors for systemic manifestation using the Brazilian Notifiable Diseases Surveillance System (2007 to 2015. RESULTS: Half of Hymenoptera injuries were caused by bee stings. Hymenoptera injuries were concentrated in Manaus, and 13.36% of cases displayed systemic signs. Delayed medical assistance (4 to 12 hours presented four times more risk for systemic manifestations. CONCLUSIONS: Simple clinical observations and history of injury are critical information for prognostic improvement.

  14. Trap-nests used by Centris (Heterocentris) terminata Smith (Hymenoptera: Apidae, Centridini) at secondary Atlantic Forest fragments, in Salvador, Bahia State; Ninhos de Centris (Heterocentris) terminata Smith (Hymenoptera: Apidae, Centridini) em fragmentos de Mata Atlantica secundaria, Salvador, BA

    Energy Technology Data Exchange (ETDEWEB)

    Drummmont, Patricia; Viana, Blandina F. [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Biologia. Lab. de Biologia e Ecologia de Abelhas (LABEA); Silva, Fabiana O. da [Faculdade Tecnologia e Ciencias (FTC), Salvador, BA (Brazil); Faculdades Jorge Amado, Savador, BA (Brazil)

    2008-05-15

    Ninety-five nests of Centris (Heterocentris) terminata Smith were collected in trap nests, during November/2001 and January/2003, at two fragments (PZGV e CFO-UFBA) of secondary Atlantic Forest, in Salvador, Bahia State (13 deg 01' W and 38 deg 30' S). The highest nest frequencies occurred from December to February (summer), with no nests foundations from August to October (winter - early spring). Two-hundred eight adults emerged from 347 brood cells, being 164 males and 116 females (1: 0.42). During the study period sex ratio was male biased ({chi}{sup 2} = 9.342; gl = 10; P < 0.05). C. terminata nested in holes with diameters 6, 8, 10 mm, but 84,2% were constructed in 8 and 10 mm. nests had one to seven cells arranged in a linear series with the cell's partitions built with a mixture of sand and resin or oil. Male is significantly smaller than female, which emerges from the first cells constructed. Immature mortality occurred in 14.1% of brood cells (n 49), of which 13.0% were due fail in development and 1.2% due to parasitism of Coelioxys sp. (Hymenoptera: Megachilidae) e Tetraonyx sp. (Coleoptera: Meloidae). In the study site, weather, mainly pluviosity, rather than natural enemies influenced seasonal population abundance. The long period of nesting activity, local abundance and usage of trap nests, suggest the potential of C. terminata for management aiming at pollination of native and cultivated plants. (author)

  15. Regular dorsal dimples and damaged mites of Varroa destructor in some Iranian honey bees (Apis mellifera)

    OpenAIRE

    Ardestani, Masoud M.; Ebadi, Rahim; Tahmasbi, Gholamhossein

    2011-01-01

    The frequency of damaged Varroa destructor Anderson and Trueman (Mesostigmata: Varroidae) found on the bottom board of hives of the honey bee, Apis mellifera L. (Hymenoptera: Apidae) has been used as an indicator of the degree of tolerance or resistance of honey bee colonies against mites. However, it is not clear that this measure is adequate. These injuries should be separated from regular dorsal dimples that have a developmental origin. To investigate damage to Varroa mites and regular dor...

  16. Nest initiation in three North American bumble bees (Bombus): gyne number and presence of honey bee workers influence establishment success and colony size.

    Science.gov (United States)

    Strange, James P

    2010-01-01

    Three species of bumble bees, Bombus appositus Cresson, Bombus bifarius, Cresson and Bombus centralis Cresson (Hymenoptera: Apidae) were evaluated for nest initiation success under three sets of initial conditions. In the spring, gynes of each species were caught in the wild and introduced to nest boxes in one of three ways. Gynes were either introduced in conspecific pairs, singly with two honey bees, Apis mellifera L. (Hymenoptera: Apidae) workers, or alone. Nesting success and colony growth parameters were measured to understand the effects of the various treatments on nest establishment. Colonies initiated from pairs of conspecific gynes were most successful in producing worker bees (59.1%), less successful were colonies initiated with honey bee workers (33.3%), and least successful were bumble bee gynes initiating colonies alone (16.7%). There was a negative correlation between the numbers of days to the emergence of the first worker in a colony to the attainment of ultimate colony size, indicating that gynes that have not commenced oviposition in 21 days are unlikely to result in colonies exceeding 50 workers. B. appositus had the highest rate of nest establishment followed by B. bifarius and B. centralis. Nest establishment rates in three western bumble bee species can be increased dramatically by the addition of either honey bee workers or a second gyne to nesting boxes at colony initiation.

  17. Single locus complementary sex determination in Hymenoptera : an "unintelligent" design?

    NARCIS (Netherlands)

    Wilgenburg, Ellen van; Driessen, Gerard; Beukeboom, Leo W.

    2006-01-01

    The haplodiploid sex determining mechanism in Hymenoptera (males are haploid, females are diploid) has played an important role in the evolution of this insect order. In Hymenoptera sex is usually determined by a single locus, heterozygotes are female and hemizygotes are male. Under inbreeding,

  18. Single locus complementary sex determination in Hymenoptera: an "unintelligent" design?

    NARCIS (Netherlands)

    van Wilgenburg, E.; Driessen, G.J.J.; Beukeboom, L.W.

    2006-01-01

    The haplodiploid sex determining mechanism in Hymenoptera (males are haploid, females are diploid) has played an important role in the evolution of this insect order. In Hymenoptera sex is usually determined by a single locus, heterozygotes are female and hemizygotes are male. Under inbreeding,

  19. Hymenoptera venom review focusing on Apis mellifera

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    P. R. de Lima

    2003-01-01

    Full Text Available Hymenoptera venoms are complex mixtures containing simple organic molecules, proteins, peptides, and other bioactive elements. Several of these components have been isolated and characterized, and their primary structures determined by biochemical techniques. These compounds are responsible for many toxic or allergic reactions in different organisms, such as local pain, inflammation, itching, irritation, and moderate or severe allergic reactions. The most extensively characterized Hymenoptera venoms are bee venoms, mainly from the Apis genus and also from social wasps and ant species. However, there is little information about other Hymenoptera groups. The Apis venom presents high molecular weight molecules - enzymes with a molecular weight higher than 10.0 kDa - and peptides. The best studied enzymes are phospholipase A2, responsible for cleaving the membrane phospholipids, hyaluronidase, which degrades the matrix component hyaluronic acid into non-viscous segments and acid phosphatase acting on organic phosphates. The main peptide compounds of bee venom are lytic peptide melittin, apamin (neurotoxic, and mastocyte degranulating peptide (MCD.

  20. KEANEKARAGAMAN HYMENOPTERA PARASITOID PADA PERKEBUNAN KELAPA SAWIT PTPN VIII CINDALI, BOGOR

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    Ichsan Luqmana Indra Putra

    2016-09-01

    Full Text Available Diversity of parasitic Hymenoptera in PTPN VIII oil palm plantation Cindali, Bogor. One group of important natural enemies in oil palm plantation is parasitic Hymenoptera. The purpose of this research is to know the diversity and fluctuation of parasitic Hymenoptera PTPN VIII Cindali oil palm plantation. This research was conducted in 6 blocks of oil palm in September 2014 – June 2015. There were 5 plots in every observation blocks 39.2 x 39.2 m in size, and used direct and indirect method. Direct method done by 5 plants in every plots was taken randomized to observed and taken the herbivore insects to reared until the parasitic Hymenoptera came out. Observation of cover crops conducted by 3 subplots determined diagonally in every plots 9.8 x 9.8 m in size and herbivore insects was observed and collected. Indirect methods used sweep net and yellow pan trap. The result of this research, 26 parasitic Hymenoptera families was found, with the Braconidae was the most morphospecies found and the most individual amount was Scelionidae. The abundance of parasitoid in every month fluctuated.

  1. Pengaruh Habitat Sekitar Lahan Persawahan dan Umur Tanaman Padi terhadap Keanekaragaman Hymenoptera Parasitika

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    Nina Herlina

    2015-09-01

    Full Text Available As the largest group of biological control agents, Parasitic Hymenoptera play important role in controlling pest outbreak in agricultural habitat. Unfortunately, there is lack of information about how these parasitoids occur in agricultural habitat related to condition of surrounding habitat and phenology of crop plant. The objective of this research was to study the effect of rice field surrounding habitat and age of rice plant on the diversity of Parasitic Hymenoptera. Research area was located in Carang Pulang Village, Dramaga, Bogor. We selected four blocks which represented different of habitat condition and age of rice plant. Each block was set six yellow pan traps (with minimum distance 20 meter and one malaise trap. Sampling of insects were conducted weekly from 6 to 12 week after planting. From this research, we collected 1,833 individual of Hymenoptera (without ants belong to 9 superfamilies, 23 families, and 216 species. Parasitic Hymenoptera was more abundant (96% and species rich (84% than Aculeata. Rice field surrounding habitat (block and age of rice field significantly affect the diversity of Parasitic Hymenoptera. We found positively correlation between age of rice plant and species richness of Parasitic Hymenoptera.

  2. Natural history of interaction between Meteorus sp. Haliday, 1835 (Hymenoptera: Braconidae) and its hyperparasitoid Toxeumella albipes Girault, 1913 (Hymenoptera: Pteromalidae).

    Science.gov (United States)

    Sobczak, J F; Maia, D P; Moura, J C M S; Costa, V A; Vasconcellos-Neto, J

    2012-02-01

    Some parasitoids build a cocoon mass that hangs in the host body until the adults emergence, which is an advantage against attack by predators who troll the vegetation in search of prey. However, such behaviour is not effective against the hyperparasitoid attacks. This study reports the interaction between the caterpillar Manduca sexta Linnaeus, 1763 (Lepidoptera, Sphingidae) parasitised by Meteorus sp. (Hymenoptera, Braconidae) larvae and its hyperparasitoid Toxeumella albipes (Hymenoptera, Pteromalidae). This is the first description of the attack and oviposition of T. albipes.

  3. Diversity of bees (Hymenoptera, Apiformes in extensive orchards in the highlands of Jordan

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    Al-Ghzawi, A.

    2014-09-01

    Full Text Available Bees visiting the blossoms of fruit trees were surveyed for the first time in Jordan. A transect was determined in Ebbin village in Ajlun (32° 22″ N, 35° 49″ E where the bees were collected from blossoms of stone fruit trees. Most of the specimens were identified up to the species level and few specimens were identified up to the genus level only. A total of 1,461 specimens were collected during the study period and 53 bee species were identified and recorded for the first time in Jordan. The collected species represented five families: Apidae, Megachilidae, Halictidae, Andrenidae and Colletidae. The results showed that Apidae and Megachilidae were the largest in terms of diversity, while Halictidae showed the highest abundance.

  4. Natural history of interaction between Meteorus sp. Haliday, 1835 (Hymenoptera: Braconidae and its hyperparasitoid Toxeumella albipes Girault, 1913 (Hymenoptera: Pteromalidae

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    JF Sobczak

    Full Text Available Some parasitoids build a cocoon mass that hangs in the host body until the adults emergence, which is an advantage against attack by predators who troll the vegetation in search of prey. However, such behaviour is not effective against the hyperparasitoid attacks. This study reports the interaction between the caterpillar Manduca sexta Linnaeus, 1763 (Lepidoptera, Sphingidae parasitised by Meteorus sp. (Hymenoptera, Braconidae larvae and its hyperparasitoid Toxeumella albipes (Hymenoptera, Pteromalidae. This is the first description of the attack and oviposition of T. albipes.

  5. Inventario de Hymenoptera (Hexapoda en El Ventorrillo: un rico enclave de biodiversidad en la Sierra de Guadarrama (España Central

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    Nieves-Aldrey, José Luis

    2003-12-01

    Full Text Available Overall data of the inventory of Hymenoptera from the Biogeological Station of “El Ventorrillo” are presented. The studied site is located at an altitude of 1450 m, on the south face of the Sierra de Guadarrama (Central Spain, about 60 km NW from Madrid. Between 1988 and 1991 an insect biodiversity inventory was carried out using three sampling methods: Malaise traps, yellow pan traps and sweep nets. Out of the more than 1,000,000 insects trapped, increasing the collections of the MNCN, about 600,000 were sorted to order. We found 83,688 individuals of Hymenoptera (representing 13,8% and the second more abundant group in the samples, after Diptera (450,000 individuals and 77,5% of total. Forty nine families, 518 genera and 1310 species de Hymenoptera has been identified until now. The overall richness of Hymenoptera from El Ventorrillo is estimated in 2700 species and about 13,000 the number of insect species from the study site. An appendix is provided with the list of identified species and its overall abundance in the samples. As results of the inventory, ten new species for science have been described, and several more new species are not yet described; additionally, at least 33 genera and more than 170 species were recorded for the first time for Iberia. The abundance of Hymenoptera, as measured by Malaise trap catches, was very high, comparatively to other published data, reaching a peak of 916 individuals per trap day at the most productive trap and sampling period. The more abundant families were, in decreasing order, Braconidae, Ichneumonidae, Mymaridae, Scelionidae, Apidae and Pteromalidae, represented by numbers of individuals between 12,000, for Braconidae, to near 6000 for Pteromalidae. Among the identified families, the more species rich at the study area were in decreasing order: Pteromalidae (290, Ichneumonidae (217, Sphecidae (107 and Eulophidae (101 species. The richness of the 29 remaining families at the area of study was

  6. Component Resolved Diagnosis in Hymenoptera Anaphylaxis.

    Science.gov (United States)

    Tomsitz, D; Brockow, K

    2017-06-01

    Hymenoptera anaphylaxis is one of the leading causes of severe allergic reactions and can be fatal. Venom-specific immunotherapy (VIT) can prevent a life-threatening reaction; however, confirmation of an allergy to a Hymenoptera venom is a prerequisite before starting such a treatment. Component resolved diagnostics (CRD) have helped to better identify the responsible allergen. Many new insect venom allergens have been identified within the last few years. Commercially available recombinant allergens offer new diagnostic tools for detecting sensitivity to insect venoms. Additional added sensitivity to nearly 95% was introduced by spiking yellow jacket venom (YJV) extract with Ves v 5. The further value of CRD for sensitivity in YJV and honey bee venom (HBV) allergy is more controversially discussed. Recombinant allergens devoid of cross-reactive carbohydrate determinants often help to identify the culprit venom in patients with double sensitivity to YJV and HBV. CRD identified a group of patients with predominant Api m 10 sensitization, which may be less well protected by VIT, as some treatment extracts are lacking this allergen. The diagnostic gap of previously undetected Hymenoptera allergy has been decreased via production of recombinant allergens. Knowledge of analogies in interspecies proteins and cross-reactive carbohydrate determinants is necessary to distinguish relevant from irrelevant sensitizations.

  7. Contribution of Plantation Forest on Wild Bees (Hymenoptera: Apoidea Pollinators Conservation in Mount Slamet, Central Java, Indonesia

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    Imam Widhiono

    2017-12-01

    Full Text Available Wild bee pollinators (Hymenoptera : Apiade diversity and abundance were studied in three types of plantation forest on Mt. Slamet (Central Java Province, Indonesia. The aims of the research was to know the diversity and abundance of wild bee pollinators and to determine the possibility of plantation forest contribution on wild bees conservation. Sampling has been done at three stands: a pine forest (PF, with Pinus merkusii, an Agathis forest (AF, with Agathis damara and a community forest (CF, with Albizia falctaria. Each habitat was divided into 5 line transect (100 x 5 m and sweep nets were used to collect the wild bee samples. Sampling was done eah month from April to August 2015. The diversity of wild bees was high (12 species in 9 genera; members of the Apidae (7 species were dominant. The most abundant species across the forests were Apis cerana (343 individuals; 25.5% of total, Trigona laeviceps (195 individuals; 14.5%, and Megachille relativa (165 individuals; 12.3%. Measurements of species diversity (H’, species evenness (E, habitat similarity (Ss and species richness indicated that the wild bee species diversity in the region was relatively high (H’ = 1.275 to (H’ = 1.730;(E= 0.870 to (E = 0.93. The result showed that the diversity of wild bees in three different plantation forest habitats on Mt. Slamet were similar and can be concluded that plantation forest types were important for pollinator conservation, and an appropriate future preservation strategy should include of the areas of all plantation forest types.

  8. Primera cita de la Argentina de Ophelimus maskelli (Ashmead (Hymenoptera: Eulophidae y su parasitoide, Closterocerus chamaeleon (Girault (Hymenoptera: Eulophidae

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    Daniel A AQUINO

    2014-01-01

    Full Text Available Se cita por primera vez de la Argentina la presencia de la avispa galí - cola exótica Ophelimus maskelli (Ashmead (Hymenoptera: Eulophidae y su para - sitoide natural, Closterocerus chamaeleon (Girault (Hymenoptera: Eulophidae en Eucalyptus camaldulensis de la provincia de Buenos Aires. Ophelimus maskelli es una especie invasora de origen Australiano que ha sido reportada como plaga de los eucaliptos en numerosos países de Asia, Europa y América. Closterocerus chamaleon es un parasitoide exótico cuyo potencial como biocontrolador de O. maskelli está siendo evaluado en algunos países.

  9. Evolución de la sociabilidad en Hymenoptera: Rasgos conductuales vinculados a niveles sociales y precursores de sociabilidad en especies solitarias Evolution of sociality in Hymenoptera: Behavioural traits linked to social levels and precursors of sociality in solitary species

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    LUIS FLORES-PRADO

    2012-09-01

    Full Text Available En Hymenoptera, los niveles de sociabilidad han sido asociados a rasgos conductuales, tales como los comportamientos de nidificación y agonísticos, y la capacidad de reconocimiento entre conespecíficos. El reconocimiento de compañeros de nido es un fenómeno de amplia difusión entre especies eusociales, y puede ser inferido por el resultado de las interacciones agonísticas entre hembras; estas son más tolerantes hacia compañeras de nido que hacia no compañeras de nido. Contrariamente, en la mayor parte de las especies solitarias las hembras son agresivas hacia otras hembras conespecíficas. En especies eusociales, la descendencia inmadura es alimentada directamente por la madre, o por obreras; así, el contacto frecuente entre progenie y hembras adultas puede contribuir a entender el reconocimiento social. En el extremo opuesto, las especies solitarias construyen nidos que no permiten interacciones entre adultos e inmaduros. A pesar de esto, estudios recientes sugieren que el aprendizaje del fenotipo propio podría explicar la capacidad de reconocimiento y, tal vez, corresponde al punto de partida en el desarrollo y evolución de la sociabilidad. La subfamilia Xylocopinae (Apidae ha emergido como un valioso modelo para estudiar la evolución de la sociabilidad pues contiene especies que presentan un amplio rango de sociabilidad. En particular, la tribu Manueliini representa un taxón interesante desde el punto de vista de la evolución de la sociabilidad en Xylocopinae pues ha sido propuesto como el grupo hermano de todos los demás Xylocopinae, es un taxón relicto que retiene rasgos morfológicos ancestrales, contiene solo especies fundamentalmente solitarias (aunque en una de estas se ha demostrado recientemente reconocimiento de compañeras de nido y de parientes y algunas especies exhiben rasgos conductuales precursores de vida social. En este trabajo se revisa en Hymenoptera los grados de sociabilidad asociados con rasgos

  10. The Community of Hymenoptera Parasitizing Necrophagous Diptera in an Urban Biotope

    Science.gov (United States)

    Frederickx, Christine; Dekeirsschieter, Jessica; Verheggen, François J.; Haubruge, Eric

    2013-01-01

    Most reports published in the field of forensic entomology are focused on Diptera and neglect the Hymenoptera community. However, Hymenoptera are part of the entomofaunal colonization of a dead body. The use of Hymenoptera parasitoids in forensic entomology can be relevant to evaluate the time of death. Hymenoptera parasitoids of the larvae and pupae of flies may play an important role in the estimation of the post-mortem period because their time of attack is often restricted to a small, well-defined window of time in the development of the host insect. However, these parasitoids can interfere with the developmental times of colonizing Diptera, and therefore a better understanding of their ecology is needed. The work reported here monitored the presence of adult Hymenoptera parasitoids on decaying pig carcasses in an urban biotope during the summer season (from May to September). Six families and six species of parasitoids were recorded in the field: Aspilota fuscicornis Haliday (Braconidae), Alysia manducator Panzer, Nasonia vitripennis Walker (Pteromalidae), Tachinaephagus zealandicus Ashmead (Encyrtidae), Trichopria sp. (Diapriidae), and Figites sp. (Figitidae). In the laboratory, five species emerged from pupae collected in the field: Trichopria sp., Figites sp., A. manducator, N. vitripennis, and T. zealandicus. These five species colonize a broad spectrum of Diptera hosts, including those species associated with decomposing carcasses, namely those from the families Calliphoridae, Muscidae, Fanniidae, and Sarcophagidae. PMID:23895458

  11. Sex determination in the Hymenoptera

    NARCIS (Netherlands)

    Heimpel, George E.; de Boer, Jetske G.

    2008-01-01

    The dominant and ancestral mode of sex determination in the Hymenoptera is arrhenotokous parthenogenesis, in which diploid females develop from fertilized eggs and haploid males develop from unfertilized eggs. We discuss recent progress in the understanding of the genetic and cytoplasmic mechanisms

  12. Diagnosis of Hymenoptera venom allergy

    NARCIS (Netherlands)

    Bilo, BM; Rueff, F; Mosbech, H; Bonifazi, F; Oude Elberink, JNG

    2005-01-01

    The purpose of diagnostic procedure is to classify a sting reaction by history, identify the underlying pathogenetic mechanism, and identify the offending insect. Diagnosis of Hymenoptera venom allergy thus forms the basis for the treatment. In the central and northern Europe vespid (mainly Vespula

  13. História natural da interação entre Meteorus sp. Haliday, 1835 (Hymenoptera: Braconidae) e seu hiperparasitoide Toxeumella albipes Girault, 1913 (Hymenoptera: Pteromalidae)

    OpenAIRE

    Sobczak, JF; Maia, DP; Moura, JCMS; Costa, VA; Vasconcellos-Neto, J

    2012-01-01

    Some parasitoids build a cocoon mass that hangs in the host body until the adults emergence, which is an advantage against attack by predators who troll the vegetation in search of prey. However, such behaviour is not effective against the hyperparasitoid attacks. This study reports the interaction between the caterpillar Manduca sexta Linnaeus, 1763 (Lepidoptera, Sphingidae) parasitised by Meteorus sp. (Hymenoptera, Braconidae) larvae and its hyperparasitoid Toxeumella albipes (Hymenoptera, ...

  14. On the identity of the adventive species of Eufriesea Cockerell in the USA: systematics and potential distribution of the coerulescens species group (Hymenoptera, Apidae

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    Victor H. Gonzalez

    2017-04-01

    Full Text Available In the summer of 2010, two male specimens of the neotropical orchid bee genus Eufriesea Cockerell were collected in the Guadalupe Mountains of western Texas and southeastern New Mexico, USA. We tentatively identified them as E. coerulescens (Lepeletier de Saint Fargeau because of the uncertainty surrounding the limits of this taxon and hypothesized that they were members of a persistent bee population, rather than long-distance transient vagrants. The goals of this paper are to clarify the identity of these specimens, assess the species limits of E. coerulescens, and to evaluate suitability of habitats in the USA for this adventive species. Herein, we revise the species in the coerulescens group using morphological features of both sexes and confirm that the specimens of Eufriesea from the USA are E. coerulescens. We recognize the following six species in the coerulescens group: E. coerulescens, E. micheneri Ayala & Engel, E. simillima (Moure & Michener, which is reinstated from synonym with E. coerulescens, and three new species from Mexico (E. barthelli Gonzalez & Griswold, sp. n., E. engeli Gonzalez & Griswold, sp. n., and E. oliveri Gonzalez & Griswold, sp. n.. To facilitate the identification of these taxa, we present a fully illustrated account of the species, comparative diagnoses, descriptions, and an updated key to all Mexican species of Eufriesea. Our analyses using species distribution modelling show an absence of suitable habitat for E. coerulescens in western Texas and southeastern New Mexico, thus favoring the long-distance dispersal hypothesis. The analyses also suggest high suitability of habitats across the Caribbean and some areas in Florida, as well as in other regions in Mexico and Central America. We discuss the implications of these results and compare them with the predicted distribution available for the other two known adventive orchid bee species in the USA.

  15. Effects of honey bee (Hymenoptera: Apidae) and bumble bee (Hymenoptera: Apidae) presence on cranberry (Ericales: Ericaceae) pollination.

    Science.gov (United States)

    Evans, E C; Spivak, M

    2006-06-01

    Honey bees, Apis mellifera L., are frequently used to pollinate commercial cranberries, Vaccinium macrocarpon Ait., but information is lacking on the relative contribution of honey bees and native bees, the effects of surrounding vegetation on bee visitation, and on optimal timing for honey bee introduction. We begin with a descriptive study of numbers of honey bees, bumble bees, and other bees visiting cranberry blossoms, and their subsequent effect on cranberry yield, on three cranberry properties in 1999. The property surrounded by agricultural land, as opposed to wetlands and woodlands, had fewer numbers of all bee types. In 2000, one property did not introduce honey bee colonies, providing an opportunity to document the effect of lack of honey bees on yield. With no honey bees, plants along the edge of the bed had significantly higher berry weights compared with nonedge plants, suggesting that wild pollinators were only effective along the edge. Comparing the same bed between 1999, with three honey bee colonies per acre, and 2000, with no honey bees, we found a significant reduction in average berry size. In 2000, we compared stigma loading on properties with and without honey bees. Significantly more stigmas received the minimum number of tetrads required for fruit set on the property with honey bees. Significantly more tetrads were deposited during mid-bloom compared with early bloom, indicating that mid-bloom was the best time to have honey bees present. This study emphasizes the importance and effectiveness of honey bees as pollinators of commercial size cranberry plantings.

  16. ABO blood groups, Rhesus factor, and anaphylactic reactions due to Hymenoptera stings.

    Science.gov (United States)

    Pałgan, Krzysztof; Bartuzi, Zbigniew; Chrzaniecka, Elżbieta

    2017-09-21

    Numerous publications indicate that the prevalence of some infectious, neoplastic and immunological diseases are associated with ABO blood groups. The aim of this study was to verify whether ABO and Rh blood groups are associated with severe anaphylactic reactions after Hymenoptera stings. A study was undertaken of 71,441 Caucasian subjects living in the same geographic area. The study group included 353 patients with diagnosed systemic anaphylaxis to Hymenoptera venom. Control group included 71,088 healthy blood donors. Frequencies of ABO and Rhesus groups in the study and control groups were compared using univariate and multivariate analyses. No statistically significant interactions were observed between the ABO blood group and anaphylactic reactions to Hymenoptera.

  17. Native Honey Bees Outperform Adventive Honey Bees in Increasing Pyrus bretschneideri (Rosales: Rosaceae) Pollination.

    Science.gov (United States)

    Gemeda, Tolera Kumsa; Shao, Youquan; Wu, Wenqin; Yang, Huipeng; Huang, Jiaxing; Wu, Jie

    2017-12-05

    The foraging behavior of different bee species is a key factor influencing the pollination efficiency of different crops. Most pear species exhibit full self-incompatibility and thus depend entirely on cross-pollination. However, as little is known about the pear visitation preferences of native Apis cerana (Fabricius; Hymenoptera: Apidae) and adventive Apis mellifera (L.; Hymenoptera: Apidae) in China. A comparative analysis was performed to explore the pear-foraging differences of these species under the natural conditions of pear growing areas. The results show significant variability in the pollen-gathering tendency of these honey bees. Compared to A. mellifera, A. cerana begins foraging at an earlier time of day and gathers a larger amount of pollen in the morning. Based on pollen collection data, A. mellifera shows variable preferences: vigorously foraging on pear on the first day of observation but collecting pollen from non-target floral resources on other experimental days. Conversely, A. cerana persists in pear pollen collection, without shifting preference to other competitive flowers. Therefore, A. cerana outperforms adventive A. mellifera with regard to pear pollen collection under natural conditions, which may lead to increased pear pollination. This study supports arguments in favor of further multiplication and maintenance of A. cerana for pear and other native crop pollination. Moreover, it is essential to develop alternative pollination management techniques to utilize A. mellifera for pear pollination. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  18. A checklist of Ropalidiini wasps (Hymenoptera: Vespidae: Polistinae in Indochina

    Directory of Open Access Journals (Sweden)

    Pham Phong Huy

    2014-01-01

    Full Text Available As a basis for intensive study of the taxonomy and biogeography of Ropalidiini wasps in Indochina (Hymenoptera: Vespidae: Polistinae, a checklist of Ropalidiini wasps (Hymenoptera: Vespidae is presented. A total of 57 Ropalidiini species and subspecies belonging to three genera from Indochina are listed, together with information of the type material deposited in the Natural History Collection, Ibaraki University, Japan (IUNH and the Institute of Ecology and Biological Resources (IEBR. References of their distribution in Indochina are also provided.

  19. A new scenario of bioprospecting of Hymenoptera venoms through proteomic approach

    Directory of Open Access Journals (Sweden)

    LD Santos

    2011-01-01

    Full Text Available Venoms represent a huge and essentially unexplored reservoir of bioactive components that may cure diseases that do not respond to currently available therapies. This review select advances reported in the literature from 2000 to the present about the new scenario of Hymenoptera venom composition. On account of new technologies in the proteomic approach, which presents high resolution and sensitivity, the combination of developments in new instruments, fragmentation methods, strategic analysis, and mass spectrometry have become indispensable tools for interrogation of protein expression, molecule interaction, and post- translational modifications. Thus, the biochemical characterization of Hymenoptera venom has become a major subject of research in the area of allergy and immunology, in which proteomics has been an excellent alternative to assist the development of more specific extracts for diagnosis and treatment of hypersensitive patients to Hymenoptera venoms.

  20. Natural history of Hymenoptera venom allergy in Eastern Spain.

    Science.gov (United States)

    Fernandez, J; Soriano, V; Mayorga, L; Mayor, M

    2005-02-01

    The natural history of stings, the clinical reaction of the patient and in vivo and in vitro tests are necessary parameters to assess before initiating Hymenoptera venom immunotherapy. In the decision to initiate immunotherapy with Hymenoptera venom, it is not usual to evaluate the natural history of the disease, which seems to be self-limiting and therefore of variable clinical significance. Our aim was to determine the natural history of Hymenoptera hypersensitivity over 4 consecutive years in a rural Mediterranean population. An epidemiological study of Hymenoptera sting reactions and possible sensitivity was carried out in 145 randomly selected subjects out of a rural Mediterranean population of 600. Seventy-two subjects, including those with a history of anaphylaxis, completed the 4-year study. The nature of their clinical reactions, age, sex, history of atopy, profession, family history of reactions to Hymenoptera insects, time elapsed since the last sting, number of stings and specific IgE and IgG were determined (the latter, to the three most important insects in the area: Apis mellifera, Polistes dominulus, and Vespula germanica). Of the 72 subjects, four subjects had systemic reactions (SR), 23 had large local reaction (LLR) and all the others (117) was minor local reactions. None who had experienced an SR had a repeat SR when re-stung over the 4-year study. Of those with LLR, 12 subjects had the same type of reaction and 11 experienced more mild local reactions when re-stung. In the SR and local reaction groups, IgE to honey bee (Hb) increased significantly during the study period, whereas in those with only LLR, specific IgE to wasp (Polistes) decreased. Specific IgG to Polistes and Vespula (wasps) decreased significantly, whereas there was no change in the specific IgG to Hb in any of the groups. The number of stings per year decreased at the end of the study in all groups, but positive-specific IgG was higher in subjects with the greatest number of

  1. Host location by ichneumonid parasitoids is associated with nest dimensions of the host bee species.

    Science.gov (United States)

    Flores-Prado, L; Niemeyer, H M

    2012-08-01

    Parasitoid fitness depends on the ability of females to locate a host. In some species of Ichneumonoidea, female parasitoids detect potential hosts through vibratory cues emanating from them or through vibrational sounding produced by antennal tapping on the substrate. In this study, we (1) describe host location behaviors in Grotea gayi Spinola (Hymenoptera: Ichneumonidae) and Labena sp. on nests of Manuelia postica Spinola (Hymenoptera: Apidae), (2) compare nest dimensions between parasitized and unparasitized nests, (3) correlate the length of M. postica nests with the number of immature individuals developing, and (4) establish the relative proportion of parasitized nests along the breeding period of M. postica. Based on our results, we propose that these parasitoids use vibrational sounding as a host location mechanism and that they are able to assess host nest dimensions and choose those which may provide them with a higher fitness. Finally, we discuss an ancestral host-parasitoid relationship between Manuelia and ichneumonid species.

  2. Allergen-specific immunotherapy of Hymenoptera venom allergy

    DEFF Research Database (Denmark)

    Schiener, Maximilian; Graessel, Anke; Ollert, Markus

    2017-01-01

    Stings of hymenoptera can induce IgE-mediated hypersensitivity reactions in venom-allergic patients, ranging from local up to severe systemic reactions and even fatal anaphylaxis. Allergic patients' quality of life can be mainly improved by altering their immune response to tolerate the venoms...... by injecting increasing venom doses over years. This venom-specific immunotherapy is highly effective and well tolerated. However, component-resolved information about the venoms has increased in the last years. This knowledge is not only able to improve diagnostics as basis for an accurate therapy......, but was additionally used to create tools which enable the analysis of therapeutic venom extracts on a molecular level. Therefore, during the last decade the detailed knowledge of the allergen composition of hymenoptera venoms has substantially improved diagnosis and therapy of venom allergy. This review focuses...

  3. Basophil-activation tests in hymenoptera allergy

    NARCIS (Netherlands)

    Dubois, Anthony E. J.; van der Heide, Sicco

    The measurement of basophil-activation markers may be useful in detecting IgIE-mediated sensitization but the relevance for application of the basophil-activation test in prediction of clinical reactivity in Hymenoptera allergy is very limited. For this reason, this test currently has no established

  4. Taxonomic and functional responses to fire and post-fire management of a Mediterranean hymenoptera community.

    Science.gov (United States)

    Mateos, Eduardo; Santos, Xavier; Pujade-Villar, Juli

    2011-11-01

    Fire is one of the commonest disturbances worldwide, transforming habitat structure and affecting ecosystem functioning. Understanding how species respond to such environmental disturbances is a major conservation goal that should be monitored using functionally and taxonomically diverse groups such as Hymenoptera. In this respect, we have analyzed the taxonomic and functional response to fire and post-fire management of a Hymenoptera community from a Mediterranean protected area. Thus, Hymenoptera were sampled at fifteen sites located in three burnt areas submitted to different post-fire practices, as well as at five sites located in peripheral unburnt pine forest. A total of 4882 specimens belonging to 33 families, which were classified into six feeding groups according to their dietary preferences, were collected. ANOVA and Redundancy Analyses showed a taxonomic and functional response to fire as all burnt areas had more Hymenoptera families, different community composition and higher numbers of parasitoids than the unburnt area. Taxonomic differences were also found between burnt areas in terms of the response of Hymenoptera to post-fire management. In general the number of parasitoids was positively correlated to the number of potential host arthropods. Parasitoids are recognized to be sensitive to habitat changes, thus highlighting their value for monitoring the functional responses of organisms to habitat disturbance. The taxonomic and functional responses of Hymenoptera suggest that some pine-forest fires can enhance habitat heterogeneity and arthropod diversity, hence increasing interspecific interactions such as those established by parasitoids and their hosts.

  5. Taxonomic and Functional Responses to Fire and Post-Fire Management of a Mediterranean Hymenoptera Community

    Science.gov (United States)

    Mateos, Eduardo; Santos, Xavier; Pujade-Villar, Juli

    2011-11-01

    Fire is one of the commonest disturbances worldwide, transforming habitat structure and affecting ecosystem functioning. Understanding how species respond to such environmental disturbances is a major conservation goal that should be monitored using functionally and taxonomically diverse groups such as Hymenoptera. In this respect, we have analyzed the taxonomic and functional response to fire and post-fire management of a Hymenoptera community from a Mediterranean protected area. Thus, Hymenoptera were sampled at fifteen sites located in three burnt areas submitted to different post-fire practices, as well as at five sites located in peripheral unburnt pine forest. A total of 4882 specimens belonging to 33 families, which were classified into six feeding groups according to their dietary preferences, were collected. ANOVA and Redundancy Analyses showed a taxonomic and functional response to fire as all burnt areas had more Hymenoptera families, different community composition and higher numbers of parasitoids than the unburnt area. Taxonomic differences were also found between burnt areas in terms of the response of Hymenoptera to post-fire management. In general the number of parasitoids was positively correlated to the number of potential host arthropods. Parasitoids are recognized to be sensitive to habitat changes, thus highlighting their value for monitoring the functional responses of organisms to habitat disturbance. The taxonomic and functional responses of Hymenoptera suggest that some pine-forest fires can enhance habitat heterogeneity and arthropod diversity, hence increasing interspecific interactions such as those established by parasitoids and their hosts.

  6. Cross-reacting carbohydrate determinants and hymenoptera venom allergy.

    Science.gov (United States)

    Brehler, Randolf; Grundmann, Sonja; Stöcker, Benedikt

    2013-08-01

    Insect venom allergy is an important cause of anaphylaxis. Venom immunotherapy assume the clear identification of the culprit insect, but this is impeded by Immunoglobulin E (IgE) antibodies to cross reactive carbohydrate determinant (CCD) epitopes of common glycoproteins. Here we give an overview about inducers, importance, and relevance of anti-N-Glycan CCD IgE antibodies. Pollen exposure and insect stings induce anti-CCD IgE antibodies interfering with in-vitro tests for allergy diagnosis due to extensive IgE cross-reactivity. Instead of being biologically active these antibodies are irrelevant for allergic reactions due to hymenoptera stings. The general response of the immune system to the ubiquitous exposure to N-glycan containing glycoproteins is still a matter of debate. CCD specific IgG antibodies in sera of bee keepers suggest tolerance induction due to high-dose exposure. Tolerance induction by pollen and food glycoproteins has not been proved. Hymenoptera stings and pollen exposure induce anti-CCD IgE. In regard to anaphylaxis due to Hymenoptera stings these antibodies are not clinically relevant, but they are important for the specificity of in-vitro tests proving insect venom allergy. The introduction of component based diagnostic IgE testing improves the specificity of in-vitro tests if proteins devoid of CCD epitopes are used.

  7. Head capsule characters in the Hymenoptera and their phylogenetic implications

    Directory of Open Access Journals (Sweden)

    Lars Vilhelmsen

    2011-09-01

    Full Text Available The head capsule of a taxon sample of three outgroup and 86 ingroup taxa is examined for characters of possible phylogenetic significance within Hymenoptera. 21 morphological characters are illustrated and scored, and their character evolution explored by mapping them onto a phylogeny recently produced from a large morphological data set. Many of the characters are informative and display unambiguous changes. Most of the character support demonstrated is supportive at the superfamily or family level. In contrast, only few characters corroborate deeper nodes in the phylogeny of Hymenoptera.

  8. Nest-mate recognition in Manuelia postica (Apidae: Xylocopinae): an eusocial trait is present in a solitary bee

    OpenAIRE

    Flores-Prado, Luis; Aguilera-Olivares, Daniel; Niemeyer, Hermann M

    2007-01-01

    In eusocial Hymenoptera, females are more tolerant towards nest-mate than towards non-nest-mate females. In solitary Hymenoptera, females are generally aggressive towards any conspecific female. Field observations of the nest biology of Manuelia postica suggested nest-mate recognition. Experiments were performed involving two live interacting females or one live female interacting with a dead female. Live females from different nests were more intolerant to each other than females from the sa...

  9. Four newly recorded species of the genus Meteorus Haliday (Hymenoptera: Braconidae: Euphorinae) from Korea

    OpenAIRE

    Hye-Rin Lee; Tae-Ho An; Deok-Seo Ku; Bong-Kyu Byun

    2017-01-01

    Four species of the genus Meteorus (Hymenoptera: Braconidae: Euphorinae) are reported for the first time from Korea: Meteorus brevicauda Thomson, 1895, Meteorus colon Haliday, 1835, Meteorus kunashiricus Belokobylskij, 1995, and Meteorus vexator Haliday, 1835. Diagnosis, distribution, and host information are provided for each species. Keywords: Braconidae, Hymenoptera, Korea, Meteorus, New record

  10. Abejas englosinas de Colombia (Hymenoptera: Apidae I. Claves ilustradas Abejas englosinas de Colombia (Hymenoptera: Apidae I. Claves ilustradas

    Directory of Open Access Journals (Sweden)

    Bonilla Gómez María Argenis

    1992-09-01

    Full Text Available One hundred and eight euglossine bee species are registered for Colombia, distributed in five genera: Aglae (1, Eufriesea (30, Euglossa (60, Eulaema (13 and Exaerete (4. New records for Colombia include: Eufriesea auripes, Ef. dressleri, Ef. fragrocara, Ef. xantha, Euglossa viridis, Eg. azureoviridis, Eg. cognata, Eg. singularis, EI. boliviensis, EI. seabrai and EI mocsaryi. Keys are given for males and females of Aglae, Exaerete, Eulaema and Eufriesea and for males of Euglossa of the 108 Colombian euglossines. A key for the subgenera of Euglossa is presented by the first time. Revisando diferentes fuentes de literatura y colecciones de abejas en Colombia y el exterior se encontró para Colombia un total de 108 especies de abejas de la subfamilia Euglossinae repartidas en 5 géneros: Aglae (1 especie, Eufriesea (30 especies, Euglossa (60 especies, Eulaema (13 especies y Exaerete (4 especies. Se registran por primera vez para Colombia 11 especies de abejas euglosinas: Eufriesea auripes, Ef. fragrocara, Ef. dressleri, Ef. xantha, Euglossa viridis, Eg. cognata, Eg. singularis, Eg. azureoviridis, Eulaema boliviensis, EI. seabrai y EI. mocsaryi. Se presentan claves para las 108 especies de los 5 géneros así, machos y hembras de Eufriesea, Exaerete y Aglae y los machos de Euglossa y por primera vez una clave para los subgéneros de Euglossa.

  11. Trap-nests used by Centris (Heterocentris) terminata Smith (Hymenoptera: Apidae, Centridini) at secondary Atlantic Forest fragments, in Salvador, Bahia State

    International Nuclear Information System (INIS)

    Drummmont, Patricia; Viana, Blandina F.; Silva, Fabiana O. da

    2008-01-01

    Ninety-five nests of Centris (Heterocentris) terminata Smith were collected in trap nests, during November/2001 and January/2003, at two fragments (PZGV e CFO-UFBA) of secondary Atlantic Forest, in Salvador, Bahia State (13 deg 01' W and 38 deg 30' S). The highest nest frequencies occurred from December to February (summer), with no nests foundations from August to October (winter - early spring). Two-hundred eight adults emerged from 347 brood cells, being 164 males and 116 females (1: 0.42). During the study period sex ratio was male biased (χ 2 = 9.342; gl = 10; P < 0.05). C. terminata nested in holes with diameters 6, 8, 10 mm, but 84,2% were constructed in 8 and 10 mm. nests had one to seven cells arranged in a linear series with the cell's partitions built with a mixture of sand and resin or oil. Male is significantly smaller than female, which emerges from the first cells constructed. Immature mortality occurred in 14.1% of brood cells (n 49), of which 13.0% were due fail in development and 1.2% due to parasitism of Coelioxys sp. (Hymenoptera: Megachilidae) e Tetraonyx sp. (Coleoptera: Meloidae). In the study site, weather, mainly pluviosity, rather than natural enemies influenced seasonal population abundance. The long period of nesting activity, local abundance and usage of trap nests, suggest the potential of C. terminata for management aiming at pollination of native and cultivated plants. (author)

  12. Four newly recorded species of the genus Meteorus Haliday (Hymenoptera: Braconidae: Euphorinae from Korea

    Directory of Open Access Journals (Sweden)

    Hye-Rin Lee

    2017-12-01

    Full Text Available Four species of the genus Meteorus (Hymenoptera: Braconidae: Euphorinae are reported for the first time from Korea: Meteorus brevicauda Thomson, 1895, Meteorus colon Haliday, 1835, Meteorus kunashiricus Belokobylskij, 1995, and Meteorus vexator Haliday, 1835. Diagnosis, distribution, and host information are provided for each species. Keywords: Braconidae, Hymenoptera, Korea, Meteorus, New record

  13. Suscetibilidade de operárias e larvas de abelhas sociais em relação à ricinina Susceptibility of workers and larvae of social bees in relation to ricinine

    Directory of Open Access Journals (Sweden)

    Débora C. Rother

    2009-03-01

    Full Text Available Muitas substâncias de origem vegetal podem ser tóxicas ou apresentar potencial inseticida. Com o objetivo de diminuir a problemática da poluição ambiental alguns estudos vêm tentando substituir os inseticidas artificiais pelos inseticidas botânicos. Ricinus communis (Euphorbiaceae apresenta uma grande variedade de substâncias sendo a ricinina o principal componente tóxico. Considerando que as abelhas são insetos benéficos por atuarem como agentes polinizadores das plantas, este estudo teve por objetivo avaliar o efeito tóxico da ricinina para as operárias e larvas de Apis mellifera (Linnaeus, 1758 (Hymenoptera, Apidae e Scaptotrigona postica (Latreille, 1907 (Hymenoptera, Meliponini. Para isso, foram realizados testes de ingestão em operárias confinadas recebendo ricinina incorporada à dieta e testes de aplicação tópica com a substância solubilizada em metanol e aplicada no pronoto das abelhas com auxílio de uma microseringa. Para as larvas foram realizados testes de ingestão e calculada sua taxa de mortalidade. Os resultados mostram atividade tóxica significativa (p Many substances of vegetal origin can be toxic or present an insecticidal potential. With the aim of decreasing the environment pollution problem, a few studies are trying to substitute synthetic insecticides with botanical ones. Ricinus communis (Euphorbiaceae presents a great variety of substances, being the ricinine the main toxic component. Considering that bees are useful as pollinator agents of plants, this study evaluates toxicity potential of ricinine on workers and larvae of Apis mellifera (Linnaeus, 1758 (Hymenoptera, Apidae and Scaptotrigona postica (Latreille, 1907 (Hymenoptera, Meliponini. In order to determine ricinine toxicity, ingestion tests were carried out with isolated workers bees that received ricinine on its diet. Furthermore, for topic tests, solutions of ricinine in methanol were applied on pronotum of worker bees with an "Agla" brand

  14. Size and shape in Melipona quadrifasciata anthidioides Lepeletier, 1836 (Hymenoptera; Meliponini

    Directory of Open Access Journals (Sweden)

    LA Nunes

    Full Text Available This study aimed to identify differences in wing shape among populations of Melipona quadrifasciata anthidioides obtained in 23 locations in the semi-arid region of Bahia state (Brazil. Analysis of the Procrustes distances among mean wing shapes indicated that population structure did not determine shape variation. Instead, populations were structured geographically according to wing size. The Partial Mantel Test between morphometric (shape and size distance matrices and altitude, taking geographic distances into account, was used for a more detailed understanding of size and shape determinants. A partial Mantel test between morphometris (shape and size variation and altitude, taking geographic distances into account, revealed that size (but not shape is largely influenced by altitude (r = 0.54 p < 0.01. These results indicate greater evolutionary constraints for the shape variation, which must be directly associated with aerodynamic issues in this structure. The size, however, indicates that the bees tend to have larger wings in populations located at higher altitudes.

  15. Size and shape in Melipona quadrifasciata anthidioides Lepeletier, 1836 (Hymenoptera; Meliponini).

    Science.gov (United States)

    Nunes, L A; Passos, G B; Carvalho, C A L; Araújo, E D

    2013-11-01

    This study aimed to identify differences in wing shape among populations of Melipona quadrifasciata anthidioides obtained in 23 locations in the semi-arid region of Bahia state (Brazil). Analysis of the Procrustes distances among mean wing shapes indicated that population structure did not determine shape variation. Instead, populations were structured geographically according to wing size. The Partial Mantel Test between morphometric (shape and size) distance matrices and altitude, taking geographic distances into account, was used for a more detailed understanding of size and shape determinants. A partial Mantel test between morphometris (shape and size) variation and altitude, taking geographic distances into account, revealed that size (but not shape) is largely influenced by altitude (r = 0.54 p < 0.01). These results indicate greater evolutionary constraints for the shape variation, which must be directly associated with aerodynamic issues in this structure. The size, however, indicates that the bees tend to have larger wings in populations located at higher altitudes.

  16. Riqueza e Abundância de Abelhas Euglossini (Hymenoptera, Apidae em Parques Urbanos de Goiânia, Goiáse em parques urbanos de Goiânia, Goiás

    Directory of Open Access Journals (Sweden)

    Vanessa Grandolfo

    2013-07-01

    Abstract. Urban environments have become a refuge for many species, including some bees of the tribe Euglossini. The group includes species with large capacity flight, so they are considered important neotropical pollinators. Euglossini is scarce information about the Cerrado, incomplete information like geographic distribution, which are endemic and which are cosmopolitan. This study aimed to identify and analyze the composition of bee species of the tribe Euglossini occurring in urban parks as to abundance, richness and attractiveness through the use of scent baits. The specimens of Euglossini were collected monthly from April to August 2008 in four urban parks in Goiânia, Goiás. The bees were sampled with the aid of scent traps, exposed from 8 am to 12 noon, containing one of the essences: salicylate methyl, benzyl acetate, cineole, vanillin. We collected 147 male orchid bees belonging to three genera and five species. There was a strong dominance of individuals Eulaema nigrita Lepeletier (95.23%, followed by Euglossa cordata (Linnaeus (2.72%. The Cineol was the essence that attracted the greatest abundance of males and methyl salicylate had the highest species richness attracted. This study is the first record of species occurrence of Exaerete smaragdina (Guérin and Euglossa imperialis Cockerell for the Midwest and Euglossa variabilis Friese for the Cerrado. The preservation of all these areas will keep bee populations of wild these regions ecological importance of maintaining the service pollination of plant species.

  17. Abelhas africanizadas Apis mellifera scutellata Lepeletier, 1836 (Hymenoptera: Apidae: Apinae exploram recursos na floresta amazônica? Do Africanized honeybees explore resources in the amazonian forest?

    Directory of Open Access Journals (Sweden)

    Marcio Luiz de Oliveira

    2005-09-01

    Full Text Available As abelhas africanas (Apis mellifera scutellata foram trazidas para o Brasil na década de 1950 e, por acidente, cruzaram-se com outras subespécies de abelhas melíferas européias introduzidas no século XIX. Isso proporcionou o surgimento de híbridos com características predominantes das abelhas africanas, tais como rusticidade e maior capacidade de enxamear, o que lhes permitiu uma rápida adaptação e expansão por quase todo continente americano. Até hoje existem controvérsias se essas abelhas, denominadas africanizadas, causam algum impacto sobre a fauna de abelhas nativas. Nas Américas, as africanizadas estão restritas a regiões de baixas altitudes e de invernos amenos; no Brasil, ocorrem principalmente em áreas urbanas e formações vegetacionais abertas ou adulteradas, sendo dificilmente vistas ou coletadas no interior de florestas densas como a amazônica. Diante dessa observação, diversas iscas foram disponibilizadas no interior de fragmentos de florestas e de florestas contínuas na Amazônia central, para testar se operárias de abelhas africanizadas seriam capazes de penetrar nos mesmos. Nenhuma operária foi vista visitando as iscas na floresta contínua ou mesmo nos fragmentos de floresta, ocorrendo visitas somente nas áreas desmatadas e capoeiras próximas. Esse resultado, além de indicar a inexistência de competição por recursos com as abelhas nativas no interior da floresta amazônica, também indica que uma apicultura em grande escala na região seria inviável, uma vez que a floresta não é sequer visitada por essas abelhas.The African honeybees (Apis mellifera scutellata were introduced accidentally to Brazil in 1956 where it subsequently interbred with other subspecies of European honeybee here introduced in the 19th century. This resulted in hybrids with predominantly African honeybee characteristics, such as higher capacity of swarming and survival in inhospitable conditions, allowing them to adapt quickly and expand throughout nearly of the Americas. Moreover, to date there is much controversy about the probable impact of these bees, called Africanized honey bees, on native bees. In the Americas, Africanized honeybees are limited to regions of low altitude and cool winters, and in Brazil they occur principally in urban areas, and open or disturbed vegetation, not occurring in the interior of dense forest such as the Amazon Forest. We offered various kinds of bait in the interior of continuous forest, and in forest fragments to verify if Africanized honeybees would be capable of penetrate in it. No Africanized honeybee workers visited any baits in continuous forest or in forest fragments, but they did so in deforested/open areas. This result indicates that there is no possibility of source competition between Africanized and native bees within Amazon forest, and also indicates that large-scale beekeeping is unlikely to succeed in this region, because forest is not explored by Africanized bees.

  18. Brachymeria pandora (Crawford (Hymenoptera, Chalcididae: a new parasitoid of Historis odius (Fabricius (Lepidoptera, Nymphalidae

    Directory of Open Access Journals (Sweden)

    Hélcio R. Gil-Santana

    2005-12-01

    Full Text Available The first record of parasitism of Brachymeria pandora (Crawford, 1914 (Hymenoptera, Chalcididae on Historis odius (Fabricius, 1775 (Lepidoptera, Nymphalidae in the State of Rio de Janeiro, Brazil is presented.Apresenta-se o primeiro registro de parasitismo de Brachymeria pandora (Crawford, 1914 (Hymenoptera, Chalcididae em Historis odius (Fabricius, 1775 (Lepidoptera, Nymphalidae, no estado do Rio de Janeiro, Brasil.

  19. In-vitro diagnostics of Hymenoptera venom allergy

    NARCIS (Netherlands)

    Rueff, F.; Vos, B.; Przybilla, B.

    In-vitro diagnostics of Hymenoptera venom allergy Patients with a history of anaphylactic sting reactions require an allergological work-up (history, in-vitro tests, and skin tests) to clarify indications on venom immunotherapy and on the type of venom to be used. To demonstrate a venom

  20. Can the Understory Affect the Hymenoptera Parasitoids in a Eucalyptus Plantation?

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    Onice Teresinha Dall'Oglio

    Full Text Available The understory in forest plantations can increase richness and diversity of natural enemies due to greater plant species richness. The objective of this study was to test the hypothesis that the presence of the understory and climatic season in the region (wet or dry can increase the richness and abundance of Hymenoptera parasitoids in Eucalyptus plantations, in the municipality of Belo Oriente, Minas Gerais State, Brazil. In each eucalyptus cultivation (five areas of cultivation ten Malaise traps were installed, five with the understory and five without it. A total of 9,639 individuals from 30 families of the Hymenoptera parasitoids were collected, with Mymaridae, Scelionidae, Encyrtidae and Braconidae being the most collected ones with 4,934, 1,212, 619 and 612 individuals, respectively. The eucalyptus stands with and without the understory showed percentage of individuals 45.65% and 54.35% collected, respectively. The understory did not represent a positive effect on the overall abundance of the individuals Hymenoptera in the E. grandis stands, but rather exerted a positive effect on the specific families of the parasitoids of this order.

  1. Can the Understory Affect the Hymenoptera Parasitoids in a Eucalyptus Plantation?

    Science.gov (United States)

    Dall’Oglio, Onice Teresinha; Ribeiro, Rafael Coelho; Ramalho, Francisco de Souza; Fernandes, Flávio Lemes; Wilcken, Carlos Frederico; de Assis Júnior, Sebastião Lourenço; Rueda, Rosa Angélica Plata; Serrão, José Eduardo; Zanuncio, José Cola

    2016-01-01

    The understory in forest plantations can increase richness and diversity of natural enemies due to greater plant species richness. The objective of this study was to test the hypothesis that the presence of the understory and climatic season in the region (wet or dry) can increase the richness and abundance of Hymenoptera parasitoids in Eucalyptus plantations, in the municipality of Belo Oriente, Minas Gerais State, Brazil. In each eucalyptus cultivation (five areas of cultivation) ten Malaise traps were installed, five with the understory and five without it. A total of 9,639 individuals from 30 families of the Hymenoptera parasitoids were collected, with Mymaridae, Scelionidae, Encyrtidae and Braconidae being the most collected ones with 4,934, 1,212, 619 and 612 individuals, respectively. The eucalyptus stands with and without the understory showed percentage of individuals 45.65% and 54.35% collected, respectively. The understory did not represent a positive effect on the overall abundance of the individuals Hymenoptera in the E. grandis stands, but rather exerted a positive effect on the specific families of the parasitoids of this order. PMID:26954578

  2. Meliponini Neotropicais: o gênero Dolichotrigona Moure (Hymenoptera, Apidae, Apinae Neotropical Meliponini: the genus Dolichotrigona Moure (Hymenoptera, Apidae, Apinae

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    João M. F. Camargo

    2005-03-01

    Full Text Available O gênero neotropical de abelhas-sem-ferrão Dolichotrigona Moure, 1950, é revisado. Sete espécies novas são descritas: D. mendersoni sp. nov. (Brasil: AM, AC, RO, D. clavicornis sp. nov. (Brasil: AC, D. rondoni sp. nov. (Brasil: RO, D. tavaresi sp. nov. (Brasil: AM, AC, D. browni sp. nov. (Brasil: RO, AC, MT; Peru, Equador, Bolívia, D. moratoi sp. nov. (Brasil, AM, AC e D. chachapoya sp. nov. (Peru. O holótipo de Trigona martinezi Brèthes, 1920, e o lectótipo de Melipona longitarsis Ducke, 1916 - aqui designado -, foram examinados e redescritos. Trigona schulthessi Friese, 1900, foi interpretada com base na literatura. Chave para identificação das espécies e ilustrações dos principais caracteres diagnósticos são apresentadas.The Neotropical stingless bees genus Dolichotrigona Moure, 1950, is revised. Seven new species are described: D. mendersoni sp. nov. (Brazil: AM, AC, RO, D. clavicornis sp. nov. (Brazil: AC, D. rondoni sp. nov. (Brazil: RO, D. tavaresi sp. nov. (Brazil: AM, AC, D. browni sp. nov. (Brazil: RO, AC, MT; Peru, Equador, Bolivia, D. moratoi sp. nov. (Brazil, AM, AC and D. chachapoya sp. nov. (Peru. The holotype of Trigona martinezi Brèthes, 1920, and the lectotype of Melipona longitarsis Ducke, 1916 - herein designated -, were examined and redescribed. Trigona schulthessi Friese, 1900, was interpreted based on the literature. Identification key for species and illustrations of the main diagnostic characters are presented.

  3. Comunidade de insetos visitantes florais de Ocimum selloi Benth (Lamiaceae em Santa Cruz do Sul, RS, Brasil

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    Karine Schoeninger

    2011-10-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2012v25n1p55 Este trabalho objetivou listar a comunidade de visitantes florais de Ocimum selloi, indicar os potenciais polinizadores e seus padrões de visitação. A coleta dos visitantes florais ocorreu no ano de 2007, em área antropofizada, sendo analisada a constância e a dominância dos táxons. Coletou-se um total de 653 insetos, pertencentes a sete ordens; Hymenoptera, com 370 indivíduos, foi o grupo mais representativo. Foram identificadas 36 famílias, sendo 12 de Hymenoptera e oito de Diptera, as mais diversas. Apidae foi a família com maior constância e dominância compreendida, principalmente, pela abelha Apis mellifera L., 1758 (n = 223, seguida de Halictidae (n = 107. A maior atividade forrageira foi observada no horário entre as 9h e 11h da manhã. Constatou-se que A. mellifera foi o principal visitante floral e potencial polinizador de O. selloi.

  4. Six new genera of Braconidae (Hymenoptera) from China

    NARCIS (Netherlands)

    Achterberg, van C.; Chen, X.

    2004-01-01

    Six new genera of the family Braconidae (Hymenoptera) from China are described and illustrated: two genera of the subfamily Agathidinae: Facilagathis gen. nov. (type species: F. spinulata spec. nov.) and Cremnoptoides gen. nov. (type species: Cremnops pappi Sharkey, 1994); one genus of the subfamily

  5. Epidemiology, diagnosis, and treatment of Hymenoptera venom allergy in mastocytosis patients.

    Science.gov (United States)

    Niedoszytko, Marek; Bonadonna, Patrizia; Oude Elberink, Joanne N G; Golden, David B K

    2014-05-01

    Hymenoptera venom allergy is a typical IgE-mediated reaction caused by sensitization to 1 or more allergens of the venom, and accounts for 1.5% to 34% of all cases of anaphylaxis. Patients suffering from mastocytosis are more susceptible to the anaphylactic reactions to an insect sting. This article aims to answer the most important clinical questions raised by the diagnosis and treatment of insect venom allergy in mastocytosis patients. Total avoidance of Hymenoptera is not feasible, and there is no preventive pharmacologic treatment available, although venom immunotherapy reduces the risk of subsequent systemic reactions. Copyright © 2014 Elsevier Inc. All rights reserved.

  6. Palmistichus elaeisis Delvare & LaSalle (Hymenoptera, Eulophidae: a new parasitoid of Dione juno juno (Cramer (Lepidoptera, Nymphalidae Palmistichus elaeisis Delvare & LaSalle (Hymenoptera, Eulophidae: um novo parasitóide de Dione juno juno (Cramer (Lepidoptera, Nymphalidae

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    Hélcio R. Gil-Santana

    2006-09-01

    Full Text Available Palmistichus elaeisis Delvare & LaSalle, 1993 (Hymenoptera, Eulophidae is recorded as parasitoid of Dione juno juno (Cramer, 1779 (Lepidoptera, Nymphalidae in the State of Rio de Janeiro, Brazil.Palmistichus elaeisis Delvare & LaSalle, 1993 (Hymenoptera, Eulophidae é registrado como parasitóide de Dione juno juno (Cramer, 1779 (Lepidoptera, Nymphalidae, no estado do Rio de Janeiro, Brasil.

  7. Provisional host catalogue of Fig wasps (Hymenoptera, Chalcidoidea)

    NARCIS (Netherlands)

    Wiebes, J.T.

    1966-01-01

    INTRODUCTION In this catalogue — entitled "provisional" because our knowledge of the subject is still so evidently incomplete — all species of Ficus mentioned as hosts of fig wasps, are listed with the Hymenoptera Chalcidoidea reared from their receptacles. The names used for the Agaonidae are in

  8. Vegetation Management and Host Density Influence Bee-Parasite Interactions in Urban Gardens.

    Science.gov (United States)

    Cohen, Hamutahl; Quistberg, Robyn D; Philpott, Stacy M

    2017-12-08

    Apocephalus borealis phorid flies, a parasitoid of bumble bees and yellow jacket wasps in North America, was recently reported as a novel parasitoid of the honey bee Apis mellifera Linnaeus (Hymenoptera: Apidae). Little is known about the ecology of this interaction, including phorid fecundity on bee hosts, whether phorid-bee parasitism is density dependent, and which local habitat and landscape features may correlate with changes in parasitism rates for either bumble or honey bees. We examined the impact of local and landscape drivers and host abundance on phorid parasitism of A. mellifera and the bumble bee Bombus vosnesenskii Radoszkowski (Hymenoptera: Apidae). We worked in 19 urban gardens along the North-Central Coast of California, where phorid parasitism of honey bees was first reported in 2012. We collected and incubated bees for phorid emergence, and surveyed local vegetation, ground cover, and floral characteristics as well as land cover types surrounding gardens. We found that phorid parasitism was higher on bumble bees than on honey bees, and phorids produced nearly twice as many pupae on individual bumble bee hosts than on honey bee hosts. Parasitism of both bumble and honey bees increased with abundance of honey bees in a site. Differences in landscape surroundings did not correlate with parasitism, but local factors related to bee resource provisioning (e.g., tree and shrub abundance) positively correlated with increased parasitism. This research thus helps to document and describe conditions that may have facilitated phorid fly host shift to honey bees and further elucidate how resource provisioning in urban gardens influences bee-parasite interactions. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  9. A new technique in the excavation of ground-nest bee burrows (Hymenoptera: Apoidea

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    Diego Marinho

    2018-01-01

    Full Text Available Bees have a diversified natural history, thus the methods applied to study such diversity are varied. When it comes to studies of nesting biology, bees which nest in pre-existing cavities have been reasonably well studied since researchers started using trap-nests. However, bees whose nests are built underground are poorly studied due to the difficulty of finding natural nesting areas and the absence of a method that facilitates bee nest excavation. The latter is evidenced by the lack of accurate descriptions in literature of how nests are excavated. In this study we tested cylindrical rubber refills of eraser pen as a new material to be used as a tracer of underground nest galleries in a natural nesting area of two species of Epicharis Klug, 1807 (Apidae. We compared this technique directly with plaster in powder form mixed with water and our results with other methodological studies describing alternative methods and materials. The rubber refill technique overcame the main issues presented by materials such as plaster, molten metal alloys and bioplastic, namely: death of the organisms by high temperatures and/or formation of plugs and materials unduly following the roots inside the galleries. Keywords: Apidae, Apoidea, Brood cells, Methodology, Solitary bees

  10. A New Species of Vespula, and First Record of Vespa crabro L. (Hymenoptera:Vespidae) from Guatemala, Central America

    Science.gov (United States)

    Vespula akrei Landolt sp. nov. (Hymenoptera:Vespidae; Vespinae) is described from Guatemala. The first record of Vespa crabro L. (Hymenoptera:Vespidae:Vespinae) in Guatemala is given, and Vespula Inexspectata Eck (1994) from Mexico is re-described. We place Vespula akrei sp. nov. in the Vespula vulg...

  11. Dual Effect of Phenolic Nectar on Three Floral Visitors of Elsholtzia rugulosa (Lamiaceae) in SW China.

    Science.gov (United States)

    Zhang, Feng-Ping; Yang, Qiu-Yun; Zhang, Shi-Bao

    2016-01-01

    Some plants secrete toxic nectar to appeal to most effective pollinators and deter non-pollinators or nectar thieves; however available information about ecological function of toxic nectar remains scarce. Elsholtzia rugulosa stands out as a plant with toxic nectar recorded in SW China. We focused on the functional significance of the phenolic compound that imparts toxic to the nectar of E. rugulosa. The effects of phenolic nectar were studied in three visitors of the flowers of the winter-blooming E. rugulosa Hemsl. (Lamiaceae) in SW China. The pollinating species Apis cerana Fabricius (Apidae; Asian honey bee) and two occasional visitors, Vespa velutina Lepeletier (Vespidae; yellow-legged Asian hornet) and Bombus eximius Smith (Apidae; a bumble bee) were tested for their preferences for low and high concentrations of 4-hydroxybenzoic acid in hexose and sucrose solutions. The pollinator is important for the plant, which is dependent on pollinator visits to attain a higher seed production and it is most likely that the combination of phenolic toxic nectar and the adaptation to phenolic nectar by A. cerana delivers an evolutionary advantage to both actors. The low and high concentrations of the phenolic acid were nearly totally refused by both occasional visitors V. velutina and B. eximius and were preferred by the pollinator A. cerana. E. rugulosa gains by having a much higher seed production and the pollinating honey bee by having an exclusive and reliable food source during the winter season at high altitudes in SW China. We found that the function of the toxic phenolic compound has dual roles by appealing to legitimate pollinators and deterring non-pollinators of E. rugulosa.

  12. Dual Effect of Phenolic Nectar on Three Floral Visitors of Elsholtzia rugulosa (Lamiaceae in SW China.

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    Feng-Ping Zhang

    Full Text Available Some plants secrete toxic nectar to appeal to most effective pollinators and deter non-pollinators or nectar thieves; however available information about ecological function of toxic nectar remains scarce. Elsholtzia rugulosa stands out as a plant with toxic nectar recorded in SW China. We focused on the functional significance of the phenolic compound that imparts toxic to the nectar of E. rugulosa. The effects of phenolic nectar were studied in three visitors of the flowers of the winter-blooming E. rugulosa Hemsl. (Lamiaceae in SW China. The pollinating species Apis cerana Fabricius (Apidae; Asian honey bee and two occasional visitors, Vespa velutina Lepeletier (Vespidae; yellow-legged Asian hornet and Bombus eximius Smith (Apidae; a bumble bee were tested for their preferences for low and high concentrations of 4-hydroxybenzoic acid in hexose and sucrose solutions. The pollinator is important for the plant, which is dependent on pollinator visits to attain a higher seed production and it is most likely that the combination of phenolic toxic nectar and the adaptation to phenolic nectar by A. cerana delivers an evolutionary advantage to both actors. The low and high concentrations of the phenolic acid were nearly totally refused by both occasional visitors V. velutina and B. eximius and were preferred by the pollinator A. cerana. E. rugulosa gains by having a much higher seed production and the pollinating honey bee by having an exclusive and reliable food source during the winter season at high altitudes in SW China. We found that the function of the toxic phenolic compound has dual roles by appealing to legitimate pollinators and deterring non-pollinators of E. rugulosa.

  13. Epidemiological study of the prevalence of allergic reactions to Hymenoptera in a rural population in the Mediterranean area.

    Science.gov (United States)

    Fernandez, J; Blanca, M; Soriano, V; Sanchez, J; Juarez, C

    1999-08-01

    Systemic allergic reactions to Hymenoptera venom occur in a percentage that varies from 0.4 to 3.3%. Epidemiological studies indicate that from 15 to 25% of the general population can be sensitized to different Hymenoptera venom as well as the fact that the degree of exposure may be related to the prevalence found in those studies. The objective of this study was to evaluate the prevalence of insect sting allergy and the venom sensitization in a rural population to three Hymenoptera previously found in the area: Polistes dominulus (Pd), Vespula germanica (Vg) and honey bee (Hb). A rural community located in the south-east of Spain, close to the Mediterranean Sea, was selected since the stinging Hymenoptera having been previously identified. A random sample of 310 subjects from the village census was studied. A questionnaire and a serum sample were obtained from every patient. The evaluation was conducted by a family doctor, who focused on the reactions to Hymenoptera sting, age, sex, occupation, atopia, previous Hymenoptera sting, stinging insect, interval to last sting and average stings per year. RAST to Hymenoptera venoms were also determined. The prevalence of systemic reactions was 2.3% (57.6% of them had a positive RAST). Large local reactions were found in 26.4% (only 28.5% of them had a positive RAST). Asymptomatic sensitization (positive RAST) was observed in 16.4% of subjects without reaction. Only a weak correlation between subjects with less than 3 years' interval to last sting exposure and positive RAST results was noted, whether they presented with a clinical reaction or not (P < 0.05). The prevalence of systemic sting reactions in our rural community is higher than other general populations in the same Mediterranean area, and similar to other rural populations studied. The degree of exposure influences not only the prevalence found but also the detection of specific serum IgE.

  14. Morphology and function of the ovipositor mechanism in Ceraphronoidea (Hymenoptera, Apocrita

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    Andrew Ernst

    2013-08-01

    Full Text Available The ovipositor of apocritan Hymenoptera is an invaluable source of phylogenetically relevant characters, and our understanding of its functional morphology stands to enlighten us about parasitoid life history strategies. Although Ceraphronoidea is one of the most commonly collected Hymenoptera taxa with considerable economic importance, our knowledge about their natural history and phylogenetic relationships, both to other apocritan lineages and within the superfamily itself, is limited. As a first step towards revealing ceraphronoid natural diversity we describe the skeletomuscular system of the ceraphronoid ovipositor for the first time. Dissections and Confocal Laser Scanning Microscopy 3D media files were used to visualize the ovipositor complex and to develop character concepts. Morphological structures were described in natural language and then translated into a character-character state format, whose terminology was linked to phenotype-relevant ontologies. Four unique anatomical phenotypes were revealed: 1. The first valvifer (gonangulum of the genus Trassedia is composed of two articulating sclerites, a condition present only in a few basal insect taxa. The bipartition of the first valvifer in Trassedia is most likely secondary and might allow more rapid oviposition. 2. Ceraphronoids, unlike other Hymenoptera, lack the retractor muscle of the terebra; instead the egg laying device is retracted by the seventh sternite. 3. Also unlike other Hymenoptera, the cordate apodeme and the anterior flange of the second valvifer are fused and compose one ridge that serves as the site of attachment for the dorsal and ventral T9-second valvifer muscles. Overall, the ceraphronoid ovipositor system is highly variable and can be described by discrete, distinguishable character states. However, these differences, despite their discrete nature, do not reflect the present classification of the superfamily and might represent parallelisms driven by host

  15. [Prevalence of hymenoptera sting allergy in veterinary medicine students from Monterey, Nuevo Leon, Mexico].

    Science.gov (United States)

    Arias Cruz, Alfredo; Monsiváis Toscano, Gina; Gallardo Martínez, Gabriela; González Díaz, Sandra Nora; Galindo Rodríguez, Gabriela

    2007-01-01

    The reported prevalence of allergic systemic reactions to hymenoptera venom occur in up to 3.3% and large local reactions occur in 17% in the general population. To investigate the prevalence of hymenoptera sting allergy in a group of veterinary medicine students from Monterrey, Nuevo Leon, Mexico. A transverse and observational study was done with 64 students of veterinary medicine. We conducted a questionnaire about the students' history of insect allergy and atopy. Skin test with allergenic extracts of bee and ant were practiced to all subjects. We performed aeroallergen skin prick test to the subjets with suspected atopy. Students age ranged from 17 to 25 years (mean 20.2) and 37 were males. Twenty students (31.3%) had clinical history of atopy and positive skin tests to aeroallergens. On the other hand, 5 students (7.8%), including 2 atopic, had suffered large local reactions, but none of them had suffered systemic reactions. Bee and ant skin tests were positive in 15.6% and 31.3% of the students respectively. There was no difference in the prevalence of hymenoptera allergy between atopic and non atopic subjects (p < 0.05). Further, the frequency of atopy in subjects with positive skin tests for bee and ant was 50%. The prevalence of large local reactions and hymenoptera sensitization found in this group was similar to that found in other epidemiologic studies.

  16. Aspectos da biologia de Euplusia violacea (Blanchard (Hymenoptera, Apidae, Euglossini Aspects of biology of Euplusia violacea (Blanchard (Hymenoptera, Apidae, Euglossini

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    Rui Carlos Peruquetti

    1997-01-01

    Full Text Available Nests of orchid bee Euplusia violacea (Blanchard, 1840 colleted in trap-nests and in the wood of a construction in Viçosa, Minas Gerais (20º45'30"S, 42º52'05"W, Brazil, were studied under laboratory condictions. Some aspects of the biology of these bees were observed, such as sex-ratio, development time, Iongevity and others. Some aspects of the behavior of E. violada were also studied.

  17. Apomictic parthenogenesis in a parasitoid wasp Meteorus pulchricornis, uncommon in the haplodiploid order Hymenoptera.

    Science.gov (United States)

    Tsutsui, Y; Maeto, K; Hamaguchi, K; Isaki, Y; Takami, Y; Naito, T; Miura, K

    2014-06-01

    Although apomixis is the most common form of parthenogenesis in diplodiploid arthropods, it is uncommon in the haplodiploid insect order Hymenoptera. We found a new type of spontaneous apomixis in the Hymenoptera, completely lacking meiosis and the expulsion of polar bodies in egg maturation division, on the thelytokous strain of a parasitoid wasp Meteorus pulchricornis (Wesmael) (Braconidae, Euphorinae) on pest lepidopteran larvae Spodoptera litura (Fabricius) (Noctuidae). The absence of the meiotic process was consistent with a non-segregation pattern in the offspring of heterozygous females, and no positive evidence was obtained for the induction of thelytoky by any bacterial symbionts. We discuss the conditions that enable the occurrence of such rare cases of apomictic thelytoky in the Hymenoptera, suggesting the significance of fixed heterosis caused by hybridization or polyploidization, symbiosis with bacterial agents, and occasional sex. Our finding will encourage further genetic studies on parasitoid wasps to use asexual lines more wisely for biological control.

  18. Transcript levels of ten caste-related genes in adult diploid males of Melipona quadrifasciata (Hymenoptera, Apidae: a comparison with haploid males, queens and workers

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    Andreia A. Borges

    2011-01-01

    Full Text Available In Hymenoptera, homozygosity at the sex locus results in the production of diploid males. In social species, these pose a double burden by having low fitness and drawing resources normally spent for increasing the work force of a colony. Yet, diploid males are of academic interest as they can elucidate effects of ploidy (normal males are haploid, whereas the female castes, the queens and workers, are diploid on morphology and life history. Herein we investigated expression levels of ten caste-related genes in the stingless bee Melipona quadrifasciata, comparing newly emerged and 5-day-old diploid males with haploid males, queens and workers. In diploid males, transcript levels for dunce and paramyosin were increased during the first five days of adult life, while those for diacylglycerol kinase and the transcriptional co-repressor groucho diminished. Two general trends were apparent, (i gene expression patterns in diploid males were overall more similar to haploid ones and workers than to queens, and (ii in queens and workers, more genes were up-regulated after emergence until day five, whereas in diploid and especially so in haploid males more genes were down-regulated. This difference between the sexes may be related to longevity, which is much longer in females than in males.

  19. Transcript levels of ten caste-related genes in adult diploid males of Melipona quadrifasciata (Hymenoptera, Apidae) - A comparison with haploid males, queens and workers.

    Science.gov (United States)

    Borges, Andreia A; Humann, Fernanda C; Oliveira Campos, Lucio A; Tavares, Mara G; Hartfelder, Klaus

    2011-10-01

    In Hymenoptera, homozygosity at the sex locus results in the production of diploid males. In social species, these pose a double burden by having low fitness and drawing resources normally spent for increasing the work force of a colony. Yet, diploid males are of academic interest as they can elucidate effects of ploidy (normal males are haploid, whereas the female castes, the queens and workers, are diploid) on morphology and life history. Herein we investigated expression levels of ten caste-related genes in the stingless bee Melipona quadrifasciata, comparing newly emerged and 5-day-old diploid males with haploid males, queens and workers. In diploid males, transcript levels for dunce and paramyosin were increased during the first five days of adult life, while those for diacylglycerol kinase and the transcriptional co-repressor groucho diminished. Two general trends were apparent, (i) gene expression patterns in diploid males were overall more similar to haploid ones and workers than to queens, and (ii) in queens and workers, more genes were up-regulated after emergence until day five, whereas in diploid and especially so in haploid males more genes were down-regulated. This difference between the sexes may be related to longevity, which is much longer in females than in males.

  20. Abridged life tables for Cephalonomia stephanoderis and Prorops nasuta (Hymenoptera: Bethylidae) Parasitoids of Hypothenemus hampei (Coleoptera: Scolytidae) reared on artificial diet

    Science.gov (United States)

    Biological aspects and demographic parameters of Cephalonomia stephanoderis Betrem (Hymenoptera: Bethylidae) and Prorops nasuta Waterston (Hymenoptera: Bethylidae) parasitoids of the coffee berry borer (CBB), Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae) were investigated usi...

  1. New faunistic data on the family Braconidae (Hymenoptera from Korea

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    Tae-Ho An

    2014-12-01

    Full Text Available Twenty-one species of the four genera in the family Braconidae (Hymenoptera are reported for the first time in Korea. These species belong to the subfamily Euphorinae (Braconidae. Faunistic and distribution data are included.

  2. The spatial distribution of Hymenoptera parasitoids in a forest reserve in Central Amazonia, Manaus, AM, Brazil

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    RB. Querino

    Full Text Available Parasitoids are of great importance to forest ecosystems due to their ecological role in the regulation of the population of other insects. The species richness and abundance of parasitoids in the forest canopy and understory, both on the borders and in the interior of a tropical forest reserve in Central Amazonia were investigated. For a 12-month period, specimen collections were made every 15 days from suspended traps placed in the forest canopy and in the understory strata, both on the border and in the interior of forest areas. A total of 12,835 Hymenoptera parasitoids from 23 families were acquired. Braconidae, Diapriidae, Mymaridae, Eulophidae, and Scelionidae were the most represented in the area and strata samples. The results indicate that there were no significant differences in the species richness or abundance of Hymenoptera between the forest borders and the inner forest. The data does show that the presence of Hymenoptera is significantly greater in the understory in both the border and interior areas than in the canopy (vertical stratification. Aphelinidae and Ceraphronidae were significantly associated with the inner forest, while the other seven families with the border of the reserve. The abundance of Hymenoptera parasitoids presented seasonal variations during the year related to the rainy and dry seasons.

  3. Flight activity of Melipona asilvai Moure (Hymenoptera: Apidae

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    B. A. Souza

    Full Text Available Many stingless bee species are specific to their areas of occurrence. Even when adapted to their local climate and flora conditions, they are subject to modifications in the environment, directly influencing flight activity. The aim of this work is to obtain information about the flight activity of the stingless bee Melipona asilvai Moure, thus contributing to the knowledge of this species. The flow of bees entering and leaving the colony was evaluated, and the type of transported material was identified. This information was correlated with climatic data collected at the time of observations, performed between June 2002 and March 2003. It can be proved that temperature was the factor with the greatest influence on the external activity of this species, showing a significant positive correlation with the entry of bees into the colony and pollen collection. Mud collecting showed a significant positive correlation with a humidity increase. Flight activity began at a temperature of 21.0 °C and humidity of 84.5%, peaking at 27.4 °C and 60.6% RH, respectively.

  4. Flight activity of Melipona asilvai Moure (Hymenoptera: Apidae).

    Science.gov (United States)

    Souza, B A; Carvalho, C A L; Alves, R M O

    2006-05-01

    Many stingless bee species are specific to their areas of occurrence. Even when adapted to their local climate and flora conditions, they are subject to modifications in the environment, directly influencing flight activity. The aim of this work is to obtain information about the flight activity of the stingless bee Melipona asilvai Moure, thus contributing to the knowledge of this species. The flow of bees entering and leaving the colony was evaluated, and the type of transported material was identified. This information was correlated with climatic data collected at the time of observations, performed between June 2002 and March 2003. It can be proved that temperature was the factor with the greatest influence on the external activity of this species, showing a significant positive correlation with the entry of bees into the colony and pollen collection. Mud collecting showed a significant positive correlation with a humidity increase. Flight activity began at a temperature of 21.0 degrees C and humidity of 84.5%, peaking at 27.4 degrees C and 60.6% RH, respectively.

  5. Handling sticky resin by stingless bees (Hymenoptera, Apidae

    Directory of Open Access Journals (Sweden)

    Markus Gastauer

    2011-06-01

    Full Text Available For their nest defense, stingless bees (Meliponini collect plant resins which they stick on intruders like ants or cleptobiotic robber bees causing their immobilization. The aim of this article is to identify all parts of stingless bee workers contacting these sticky resins. Of special interest are those body parts with anti-adhesive properties to resin, where it can be removed without residues. For that, extensive behavioral observations during foraging flight, handling and application of the resin have been carried out. When handling the resin, all tarsi touch the resin while walking above it. For transportation from plants to the nest during foraging flight, the resin is packed to the corbicula via tarsi and basitarsi of front and middle legs. Once stuck to the resin or after the corbicula had been unloaded, the bee's legs have to be cleaned thoroughly. Only the tips of the mandibles, that form, cut and apply the sticky resin, seem to have at least temporarily resin-rejecting properties.

  6. Mieren in Veluwebermen: soortenrijkdom en aanbevelingen voor beheer (Hymenoptera: Formicidae).

    NARCIS (Netherlands)

    Noordijk, J.; Boer, P.

    2007-01-01

    Ants in roadside verges on the Veluwe: species richness and recommendations for management (Hymenoptera: Formicidae) Highway verges in the Veluwe region contain some well developed nutrient poor plant communities, like grasslands, grey hair grass vegetation and heather vegetation. These places

  7. A preliminary checklist of the ants (Hymenoptera: Formicidae) of ...

    African Journals Online (AJOL)

    A preliminary species checklist of the ants (Hymenoptera: Formicidae) of. Kakamega Forest, Western Kenya, is presented. The species list is based on specimens sampled from 1999 until 2009, which are deposited in the ant collection of the Zoological Research Museum Koenig, Bonn, Germany, and the Natural History ...

  8. Evolution of Cuticular Hydrocarbons in the Hymenoptera: a Meta-Analysis.

    Science.gov (United States)

    Kather, Ricarda; Martin, Stephen J

    2015-10-01

    Chemical communication is the oldest form of communication, spreading across all forms of life. In insects, cuticular hydrocarbons (CHC) function as chemical cues for the recognition of mates, species, and nest-mates in social insects. Although much is known about the function of individual hydrocarbons and their biosynthesis, a phylogenetic overview is lacking. Here, we review the CHC profiles of 241 species of Hymenoptera, one of the largest and most important insect orders, which includes the Symphyta (sawflies), the polyphyletic Parasitica (parasitoid wasps), and the Aculeata (wasps, bees, and ants). We investigated whether these taxonomic groups differed in the presence and absence of CHC classes and whether the sociality of a species (solitarily vs. social) had an effect on CHC profile complexity. We found that the main CHC classes (i.e., n-alkanes, alkenes, and methylalkanes) were all present early in the evolutionary history of the Hymenoptera, as evidenced by their presence in ancient Symphyta and primitive Parasitica wasps. Throughout all groups within the Hymenoptera, the more complex a CHC the fewer species that produce it, which may reflect the Occam's razor principle that insects' only biosynthesize the most simple compound that fulfil its needs. Surprisingly, there was no difference in the complexity of CHC profiles between social and solitary species, with some of the most complex CHC profiles belonging to the Parasitica. This profile complexity has been maintained in the ants, but some specialization in biosynthetic pathways has led to a simplification of profiles in the aculeate wasps and bees. The absence of CHC classes in some taxa or species may be due to gene silencing or down-regulation rather than gene loss, as demonstrated by sister species having highly divergent CHC profiles, and cannot be predicted by their phylogenetic history. The presence of highly complex CHC profiles prior to the vast radiation of the social Hymenoptera indicates a

  9. A new species of Crinibracon Quicke (Hymenoptera: Braconidae) parasitic on pupae of Hasora chromus (Cramer) (Lepidoptera: Hesperiidae) from India.

    Science.gov (United States)

    Gupta, Ankita; Achterberg, Cornelis Van; Chitrala, Malathi

    2016-08-29

    A new species, Crinibracon chromusae Gupta & van Achterberg sp. n., parasitic on pupae of Hasora chromus (Cramer) (Hesperiidae) on Millettia (= Pongamia) pinnata (L.) Panigrahi (Fabaceae), is described from India and compared with C. sinicus (Yang, Chen & Liu, 2008) from China, the only other species known with a similar general appearance. For the first time biological information for the genus Crinibracon Quicke, 1988, is given. Three species of hyperparasitoids, Philolema braconidis (Ferrière) (Hymenoptera: Eurytomidae), Nesolynx javanica Ferrière (Hymenoptera: Eulophidae), and an Eupelmus sp. (Hymenoptera: Eupelmidae) emerged along with C. chromusae sp. n. from pupae of H. chromus. The generic placement of this new species along with interesting parasitoid biology is discussed.

  10. De urntjeswesp Eumenes coronatus zoekt het hogerop (Hymenoptera: Vespidae)

    NARCIS (Netherlands)

    Smit, J.

    2005-01-01

    The potter wasp Eumenes coronatus on the move (Hymenoptera: Vespidae) The first record of Eumenus coronatus in the Netherlands dates back from 1954. After that the species was not recorded until 1988. Since then many specimens were collected in the southern part of the country. In 2004 a female was

  11. The mitochondrial genome of the stingless bee Melipona bicolor (Hymenoptera, Apidae, Meliponini: sequence, gene organization and a unique tRNA translocation event conserved across the tribe Meliponini

    Directory of Open Access Journals (Sweden)

    Daniela Silvestre

    2008-01-01

    Full Text Available At present a complete mtDNA sequence has been reported for only two hymenopterans, the Old World honey bee, Apis mellifera and the sawfly Perga condei. Among the bee group, the tribe Meliponini (stingless bees has some distinction due to its Pantropical distribution, great number of species and large importance as main pollinators in several ecosystems, including the Brazilian rain forest. However few molecular studies have been conducted on this group of bees and few sequence data from mitochondrial genomes have been described. In this project, we PCR amplified and sequenced 78% of the mitochondrial genome of the stingless bee Melipona bicolor (Apidae, Meliponini. The sequenced region contains all of the 13 mitochondrial protein-coding genes, 18 of 22 tRNA genes, and both rRNA genes (one of them was partially sequenced. We also report the genome organization (gene content and order, gene translation, genetic code, and other molecular features, such as base frequencies, codon usage, gene initiation and termination. We compare these characteristics of M. bicolor to those of the mitochondrial genome of A. mellifera and other insects. A highly biased A+T content is a typical characteristic of the A. mellifera mitochondrial genome and it was even more extreme in that of M. bicolor. Length and compositional differences between M. bicolor and A. mellifera genes were detected and the gene order was compared. Eleven tRNA gene translocations were observed between these two species. This latter finding was surprising, considering the taxonomic proximity of these two bee tribes. The tRNA Lys gene translocation was investigated within Meliponini and showed high conservation across the Pantropical range of the tribe.

  12. Knowledge about useful entomofauna in the county of La Purísima Palmar de Bravo, Puebla State, Mexico

    Directory of Open Access Journals (Sweden)

    D. H. Zetina

    2007-06-01

    Full Text Available Eighteen species of edible insects belonging to 15 families of six orders of Insecta were recorded in the county of La Purísima Palmar de Bravo in Puebla State, Mexico. They had the following taxonomic distribution among the different families: Lygaeidae, Pentatomidae, Membracidae, Cercopidae, Psocidae, Melolonthidae, Curculionidae, Cossidae, Megathymidae, Pyralidae, Geometridae and Apidae, all with a single recorded species; the families Acrididae, Formicidae and Vespidae with two species in each family. The latter were the most abundant families, and Hymenoptera was the most salient order with five species. Three species were reported as new registers of edible insects for Mexico and also for the world. The nutritive value of insects in terms of macro and micronutrients is discussed.

  13. Trophobiosis in the arboricolous ant .i.Liometopum microcephalum./i. (Hymenoptera: Formicidae: Dolichoderinae)

    Czech Academy of Sciences Publication Activity Database

    Schlaghamerský, J.; Kašpar, J.; Petráková, L.; Šustr, Vladimír

    2013-01-01

    Roč. 110, č. 2 (2013), s. 231-239 ISSN 1210-5759 Institutional support: RVO:60077344 Keywords : Hymenoptera * arboricolous * ants * trophobiosis Subject RIV: EG - Zoology Impact factor: 1.076, year: 2013

  14. On the Hymenoptera (exclusive of the Anthophila and Formicidae)

    NARCIS (Netherlands)

    Cameron, P.

    1913-01-01

    Our knowledge of the Hymenoptera of the Island of Waigeu hitherto has been based on the material collected by Dr. A. R. Wallace and described by Mr. Frederick Smith in the Journal of the Linnean Society of London, 1863, pp. 6—48. Omitting the bees (5 species) and the ants (27 species) Smith

  15. Spermatogenesis in the parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae)

    Czech Academy of Sciences Publication Activity Database

    Carabajal Paladino, Leonela Z.; Lo Nostro, F.; Papeschi, A.; Cladera, J.; Bressa, M. J.

    2017-01-01

    Roč. 98, č. 1 (2017), s. 38-43 ISSN 0001-7272 R&D Projects: GA MŠk(CZ) EE2.3.30.0032 Institutional support: RVO:60077344 Keywords : Hymenoptera * modified meiosis * abortive division Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Genetics and heredity (medical genetics to be 3) Impact factor: 1.211, year: 2016 http://onlinelibrary.wiley.com/doi/10.1111/azo.12148/pdf

  16. Utility of laboratory testing for the diagnosis of Hymenoptera venom allergy.

    Science.gov (United States)

    Vachová, Martina; Panzner, Petr; Malkusová, Ivana; Hanzlíková, Jana; Vlas, Tomáš

    2016-05-01

    A diagnosis of Hymenoptera venom allergy is based on clinical history and the results of skin tests and/or laboratory methods. To analyze the utility of available laboratory tests in diagnosing Hymenoptera venom allergy. Ninety-five patients with Hymenoptera venom allergy with a history of bee (35) or wasp (60) anaphylactic sting reaction and positive skin test with bee or wasp venom were included in this analysis. Specific immunoglobulin E (to bee venom extract, wasp venom extract, available recombinant molecules, and a basophil activation test with venom extracts were assessed in all the patients. Test sensitivity and specificity were calculated by using standard threshold values; then, receiver operating characteristic curve analysis was performed to compute optimal threshold values. Also, statistical analysis of the utility of different combinations of laboratory tests was performed. The optimal threshold values were revealed to be the following: 1.0 kIU/L for bee venom extract (sensitivity, 97.14%; specificity, 100%), 0.35 kIU/L for rApi m 1 (sensitivity, 68.57%; specificity, 100%), 1.22 kIU/L for wasp venom extract (sensitivity, 88.33%; specificity, 95.45%), 0.7 kIU/L for rVes v 5 (sensitivity, 86.67%; specificity, 95.45%), 1.0 kIU/L for rVes v 1 (sensitivity, 56.67%; specificity, 95.45%), 6.5% for basophil activation test with bee venom extract (sensitivity, 80%; specificity, 95.45%), and 4.5% for basophil activation test with wasp venom extract (sensitivity, 91.53%; specificity, 95.45%). The best test combinations were found to be the following: bee venom extract plus rApi m 1 (sensitivity, 97.14%; specificity, 95.45%) in bee and either wasp venom extract plus rVes v 5, or rVes v 5 plus rVes v 1 (both sensitivity, 98.33%; specificity, 95.45%) in patients with wasp venom allergy. Our analysis confirmed that currently used laboratory tests represent effective tools in diagnosing Hymenoptera venom allergy. Moreover, our probabilistic approach offered another

  17. Notas sistemáticas sobre abejas Meliponini del Chaco (Hymenoptera, Apidae Systematic notes on Meliponini bees of the Chaco Region (Hymenoptera, Apidae

    Directory of Open Access Journals (Sweden)

    Arturo Roig Alsina

    2010-06-01

    Full Text Available Se tratan dos especies características de la Región Chaqueña: Scaptotrigona jujuyensis (Schrottky y Lestrimelitta chacoana n. sp. Se clarifica la identidad de Trigona jujuyensis Schrottky, 1911, y se le designa un neotipo. Se presentan descripciones, ilustraciones y datos de distribución para cada especie.Two species characteristic of the Chaco region are treated in this contribution: Scaptotrigona jujuyensis (Schrottky and Lestrimelitta chacoana n. sp. The identity of Trigona jujuyensis Schrottky, 1911, is clarified and a neotype is designated. Descriptions, illustrations and distributional data are presented for each species.

  18. A review of the albidohirta group of Ptilothrix (Hymenoptera: Apidae: Emphorini Revisión del grupo albidohirta de Ptilothrix (Hymenoptera: Apidae: Emphorini

    Directory of Open Access Journals (Sweden)

    Arturo Roig Alsina

    2007-12-01

    Full Text Available The albidohirta group of Ptilothrix Smith includes three species: Ptilothrix albidohirta Brèthes, P. concolor sp. nov., and P. nemoralis sp. nov. The group is known from northern and western Argentina and from central Bolivia. One of its species, P. albidohirta, reaches high altitudes in the Andes mountains, up to 3600 m a.s.l., while the other two species occur in the lowlands. A characterization of the group, descriptions of the species, and illustrations are provided.El grupo albidohirta de Ptilothrix Smith incluye tres especies: Ptilothrix albidohirta Brèthes, P. concolor sp. nov. y P. nemoralis sp. nov. Este grupo se conoce del oeste y norte de la Argentina y del centro de Bolivia. Una de sus especies, P. albidohirta, llega hasta los 3.600 m s.n.m. en la cordillera de los Andes, en tanto que las otras dos especies habitan en el llano. Se caracteriza el grupo y se describen e ilustran las especies.

  19. An anocellar polistine wasp (Hymenoptera, Vespidae, Polistinae from Texas

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    Volker Lohrmann

    2016-12-01

    Full Text Available A remarkable teratological female of Polistes (Fuscopolistes dorsalis neotropicus Bequaert, 1940 (Vespidae: Polistinae is described and illustrated. The specimen lacks all three external dorsal ocelli but is normally developed in almost every other aspect. Additionally, similar findings in other Hymenoptera are briefly discussed, as are the consequences and the reasons that might cause the random loss of ocelli.

  20. Added sensitivity of component-resolved diagnosis in hymenoptera venom-allergic patients with elevated serum tryptase and/or mastocytosis

    DEFF Research Database (Denmark)

    Michel, J B; Brockow, K; Darsow, U

    2016-01-01

    BACKGROUND: Anaphylaxis caused by hymenoptera venom allergy is associated with elevation of baseline serum tryptase (sBT) and/or mastocytosis in about 5% of patients. Up to now, no information has become available on single venom allergen sIgE reactivity and the usefulness of component......-resolved approaches to diagnose this high-risk patient group. To address the component-resolved sIgE sensitization pattern and diagnostic sensitivity in hymenoptera venom-allergic patients with elevated sBT levels and/or mastocytosis, a panel of yellow jacket and honeybee venom allergens was applied on a widely used...... IgE immunoassay platform. METHODS: Fifty-three patients with mastocytosis and/or elevated sBT tryptase level and systemic reactions to hymenoptera venoms were analyzed for their IgE reactivity to recombinant yellow jacket and honeybee venom allergens by Immulite3 g. RESULTS: sIgE reactivity to Ves v...

  1. Protein content and electrophoretic profile of fat body and ovary extracts from workers of Melipona quadrifasciata anthidioides (Hymenoptera, Meliponini

    Directory of Open Access Journals (Sweden)

    Vagner T. Paes de Oliveira

    Full Text Available Workers of Melipona quadrifasciata anthidioides (Lepeletier, 1836 develop their ovaries and lay eggs, therefore the production of vitellogenin is expected. In electrophoretic profiles only fat body extracts from nurse workers and ovary extracts from newly-emerged workers show protein with molecular mass similar to vitellogenin. However, an increase in the protein content was detected in forager fat body. This increase was attributed to storage of vitellogenin or other proteins in the previous phase and not discharged into the hemolymph or to an effect of the increased titre of juvenile hormone in this phase of worker life over the fat body functioning.

  2. Espectro polínico de amostras de mel de Apis mellifera L., coletadas na Bahia Pollen spectrum of samples of Apis mellifera L., honey collected in Bahia State, Brazil

    Directory of Open Access Journals (Sweden)

    AUGUSTA CAROLINA DE CAMARGO CARMELLO MORETI

    2000-01-01

    Full Text Available O espectro polínico encontrado em amostras de mel provenientes de seis municípios do Estado da Bahia foi analisado com o objetivo de contribuir para o conhecimento da flora visitada por Apis mellifera L. 1758 (Hymenoptera: ApidaeA identificação dos tipos polínicos foi feita por meio de literatura especializada e de informações de campo. Foram encontrados 43 tipos de pólen, sendo consideradas como dominantes as espécies Eucalyptus sp. (Myrtaceae, Mimosa verrucosa Benth. (Mimosaceae, M. scabrella Benth. (Mimosaceae e Bauhinia sp. (Caesalpiniaceae. Os tipos acessórios foram Alternanthera ficoidea R.Br. (Amaranthaceae, tipo Compositae (Asteraceae e Cecropia sp. (Moraceae. Destacou-se a participação de Mimosa sp. (Mimosaceae e de outras espécies silvestres na composição dos méis nos municípios considerados, registrando-se ainda a espécie Eucalyptus sp. (Myrtaceae como uma das principais fontes de alimento das abelhas dessa região.Pollen spectrum found in honey samples from six localities of Bahia State, Brazil, was analyzed with the objective to contribute for the knowledge of the plants used by Apis mellifera L., 1758 (Hymenoptera: Apidae. The identification of the pollen types was made by specialized literature and by field information. Two hundred pollen grains were studied in order to determine the percentage and the occurrence of each type. Forty three pollen types were identified, being considered as the predominant types Eucalyptus sp. (Myrtaceae, Mimosa verrucosa Benth. (Mimosaceae, M. scabrella Benth. (Mimosaceae and Bauhinia sp. (Caesalpiniaceae. The accessory pollen types were Alternanthera ficoidea R.Br. (Amaranthaceae, Compositae type (Asteraceae and Cecropia sp. (Moraceae. It is intense the participation of Mimosa sp. (Mimosaceae and other wild species in the honey composition of the six localities considered. Eucalyptus sp. (Myrtaceae is one of the dominant sources of bee food in some regions of Bahia State.

  3. Uso da parafina incorporada à cera alveolada em colônias de abelhas Apis mellifera L. africanizadas para produção de mel Paraffin incorporation to beewax foundation in Apis mellifera L. Africanized colonies for honey production

    Directory of Open Access Journals (Sweden)

    Vagner de Alencar Arnaut de Toledo

    2002-04-01

    Full Text Available O objetivo foi avaliar favos construídos a partir de três tratamentos, lâminas de cera alveolada, lâminas de cera alveolada com parafina e parte do quadro sem cera. Foram utilizadas dez colônias de abelhas africanizadas, Apis mellifera L. (Hymenoptera, Apidae existentes na região de Marialva, Estado do Paraná. Foram efetuados mapeamentos da área construída e ocupada com mel, a intervalos de aproximadamente 20 dias. Três quadros, cada um contendo os três tratamentos foram colocados nas posições lateral esquerda, central e lateral direita da melgueira vista por trás da colméia. As abelhas construíram a maior área e ocuparam com mel (p The aim of this paper was the evaluation of combs from three experimental treatments: beeswax foundation, beeswax with paraffin and a gap frame, partially without wax. The experiment was carried out using ten Apis mellifera L. (Hymenoptera, Apidae Africanized honeybee colonies in Marialva - state of Paraná, in the south of Brazil. They were accomplished by mapping areas constructed and occupied with honey, at intervals of approximately 20 days. Three combs, containing, each one, the three treatments were placed at the positions right lateral, central and left lateral of the super observed the hive to the back. The honeybees built the greatest area and filled with honey (p < 0.05 when the comb was introduced in the central position of the hive, compared to other treatments in which differences were also observed (p < 0.05. The position of the wax comb mixed with paraffin produced the smallest area (p < 0.05 occupied with honey compared to the others. Results showed that paraffin mixed with wax comb should be used since the combs are placed in the central position and must be used for production of honey in sections.

  4. Revision of charipine aphid hyperparasitoids (Hymenoptera, Cynipoidea: Figitidae) from central Europe

    Czech Academy of Sciences Publication Activity Database

    Ferrer-Suay, M.; Starý, Petr; Selfa, J.; Pujade-Villar, J.

    2017-01-01

    Roč. 28, č. 3 (2017), s. 113-147 ISSN 0785-8760 Institutional support: RVO:60077344 Keywords : Hymenoptera * central Europe * aphid Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 0.300, year: 2016 http://www.entomologicafennica.org/Volume28/EF_28_3/1Ferrer-Suay.pdf

  5. Revision of the world species of Xeris Costa (Hymenoptera: Siricidae)

    Science.gov (United States)

    Henri Goulet; Caroline Boudreault; Nathan M. Schiff

    2015-01-01

    Xeris is one of ten extant genera of Siricidae known as as woodwasps or horntails. They are important wood-boring Hymenoptera from the Northern Hemisphere. Adults and larvae of Xeris are often intercepted at ports and are consequently of concern as potential alien invasive species. The genus consists of 16 species with eight in...

  6. Primer registro de Tremex fuscicornis (Hymenoptera: Siricidae para la Argentina en una plantación de álamos en Buenos Aires First record of Tremex fuscicornis (Hymenoptera: Siricidae in Argentina infesting a poplar plantation in Buenos Aires

    Directory of Open Access Journals (Sweden)

    Lucas Landi

    2011-12-01

    Full Text Available La presencia de la "avispa taladradora de las latifoliadas", Tremex fuscicornis Fabricius (Hymenoptera: Siricidae, fue detectada en una plantación de álamos de la provincia de Buenos Aires, lo que constituye el primer registro de la especie para la Argentina. Se describen aspectos morfológicos y biológicos de la misma.The presence of the woodwasp Tremex fuscicornis Fabricius (Hymenoptera: Siricidae was detected in a poplar plantation in Buenos Aires. This is the first record of the species in Argentina. Morphological and biological aspects of the woodwasp are described.

  7. First record of Aleiodes laphygmae (Hymenoptera: Braconidae) for Argentina and its association with larvae of Spodoptera eridania (Lepidoptera: Noctuidae)

    OpenAIRE

    Valverde, Liliana; Berta, D. Carolina; Gomez, Marcelo Geronimo

    2012-01-01

    Se reporta por primera vez para Argentina Aleiodes laphygmae (Viereck) (Hymenoptera: Braconidae) y como parasitoide de larvas de Spodoptera eridania (Stoll) (Lepidoptera: Noctuidae), en plantaciones de soja en la provincia de Tucumán (Argentina). Se provee información biológica como hábitos, hospedadores y distribución.Aleiodes laphygmae (Viereck) (Hymenoptera: Braconidae), is reported for the first time for Argentina. It is also reported parasitizing larvae of Spodoptera eridania (Stoll) (Le...

  8. Partial cytochrome b sequences for six Hymenoptera of the eastern United States.

    Science.gov (United States)

    Collins, A M; Gardner, L M

    2001-01-01

    Mitochondrial DNA (mtDNA) haplotypes have been commonly used to determine honeybee subspecies relationships. To see if these markers would also be useful for comparisons of other Hymenoptera, we collected workers of six local species: Vespa crabro, the European hornet; Bombus impatiens, a bumblebee; Vespula germanica, the German yellow jacket; Polistes fuscatus, a paper wasp; Halictus ligatus, an alkali bee; and an unspecified Megachile, a leafcutting bee. MtDNA was isolated and digested with six endonucleases (AvaI, BglII, EcoRI, HindIII, HinfI, XbaI). The digested DNA was electrophoresed and visualized on agarose gels with comparison to a standard fragment marker and similarly treated honeybee mtDNA. The fragments obtained were also purified and sequenced. Phylogenetic relationships between six wasp and bee species, Apis mellifera, and several other similar aculeate Hymenoptera were determined. Newly defined DNA sequences were posted to GenBank (AF281169-AF281174).

  9. Improved sensitivity to venom specific-immunoglobulin E by spiking with the allergen component in Japanese patients suspected of Hymenoptera venom allergy

    Directory of Open Access Journals (Sweden)

    Naruo Yoshida

    2015-07-01

    Conclusions: The measurement of sIgE following spiking of rVes v 5 and rPol d 5 by conventional testing in Japanese subjects with sIgE against hornet and paper wasp venom, respectively, improved the sensitivity for detecting Hymenoptera venom allergy. Improvement testing for measuring sIgE levels against hornet and paper wasp venom has potential for serologically elucidating Hymenoptera allergy in Japan.

  10. Epidemiology, Diagnosis, and Treatment of Hymenoptera Venom Allergy in Mastocytosis Patients

    NARCIS (Netherlands)

    Niedoszytko, Marek; Bonadonna, Patrizia; Oude Elberink, Joanne N. G.; Golden, David B. K.

    Hymenoptera venom allergy is a typical IgE-mediated reaction caused by sensitization to 1 or more allergens of the venom, and accounts for 1.5% to 34% of all cases of anaphylaxis. Patients suffering from mastocytosis are more susceptible to the anaphylactic reactions to an insect sting. This article

  11. Stiff upper lip: Labrum deformity and functionality in bees (Hymenoptera: Apoidea)

    Science.gov (United States)

    In hyper-diverse groups such as Hymenoptera, a variety of structures with different, complementary functions are used for feeding. Although the function of the parts such as the mandibles is obvious, the use of others, like the labrum, is more difficult to discern. Here, we discuss the labrum’s func...

  12. Bees of the Azores: an annotated checklist (Apidae, Hymenoptera).

    Science.gov (United States)

    Weissmann, Julie A; Picanço, Ana; Borges, Paulo A V; Schaefer, Hanno

    2017-01-01

    We report 18 species of wild bees plus the domesticated honeybee from the Azores, which adds nine species to earlier lists. One species, Hylaeus azorae , seems to be a single island endemic, and three species are possibly native ( Colletes eous , Halictus villosulus , and Hylaeus pictipes ). All the remaining bee species are most likely accidental introductions that arrived after human colonization of the archipelago in the 15 th century. Bee diversity in the Azores is similar to bee diversity of Madeira and Cape Verde but nearly ten times lower than it is in the Canary Islands.

  13. Bees of the Azores: an annotated checklist (Apidae, Hymenoptera

    Directory of Open Access Journals (Sweden)

    Julie A. Weissmann

    2017-01-01

    Full Text Available We report 18 species of wild bees plus the domesticated honeybee from the Azores, which adds nine species to earlier lists. One species, Hylaeus azorae, seems to be a single island endemic, and three species are possibly native (Colletes eous, Halictus villosulus, and Hylaeus pictipes. All the remaining bee species are most likely accidental introductions that arrived after human colonization of the archipelago in the 15th century. Bee diversity in the Azores is similar to bee diversity of Madeira and Cape Verde but nearly ten times lower than it is in the Canary Islands.

  14. Effects of Apis mellifera adansonii, L. 1758 (Apidae: Hymenoptera ...

    African Journals Online (AJOL)

    pollination on yields of Cucumeropsis mannii (Naudin) in Kisangani, ... 1Unit of Functional and Evolutionary Entomology, University of Liège, ... number of fruits per plant, average weight of seeds extracted per fruit, ... enhance symbiotic interactions Apis mellifera with C. mannii in DRC. ..... Trends in Ecology and Evolution,.

  15. Visitantes florais de Erythrina speciosa Andr. (Leguminosae Flowering visitors of Erythrina speciosa Andr., Leguminosae

    Directory of Open Access Journals (Sweden)

    Maria J. Vitali-Veiga

    2000-06-01

    Full Text Available Inspite of Etythrina species exhibit morphologic attributes for adaptation to pollination by nectarivorous birds mentioned in the literature, E. speciosa is pollinated by lots of bees (Apinae and Meliponinae which show a great urban occurrence. Systems of E. speciosa floral reproduction, fenology, diversity, frequency and constancy of insects visiting at different hours and flowering periods were studied. E. speciosa is Biocompatible, but xenogamy is the predominant system of reproduction. A large diversity of insects visiting the inflorescences was observed, with predominance of bees. The bee species showed a higher frequency: Apis mellifera Linnaeus, 1758 (45,0 %, Trigona spinipes (Fabricius, 1793 (28,6%, Trigona hyalinata (Lepeletier, 1836 (12,2 % and the ant Zacryptoceruspusillus Klug, 1824 (2,8 %. Constant but not frequent were the bees (Apidae Plebeia droryana (Friese, 1900, Friesella schrottkyi (Friese, 1900, Nannotrigona testaceicornis (Lepeletier, 1836, Tetragonisca angustula (Latreille, 1811, the wasps (Vespidae Polybia paulista Ihering, 1896, Protopolybia exigua (de Saussure, 1854, Agelaia pallipes (Olivier. 1791, the ant (Formicidae Pseudomyrmex sp. and the beetle (Chrysomelidae Diabrotica speciosa (Germar, 1824. E. speciosa flowers were visited by hummingbirds (Trochilidae: Eupetomena macroura (Gmelin, 1788, Clorostilbon aureoventris (d'Orbigny & Lafresnaye, 1838 and Amazilia sp. The birds Passer domeslicus (Linnaeus, 1758 (Ploceidae and Coereba flaveola (Linnaeus, 1758 (Emberizidac, also are present. The frequency and insect distribution were influenced by ambiental factors. Temperature, light, time, barometric pressure, relative humidity and wind velocity were significantly correlated with insect numbers. There is a visit sequence, by floral resource disponibility during the day, conditioned by transport ability, insect numbers and colony necessity, which begins by A. mellifera followed by meliponid bees. These bees make the

  16. Alocanthedon, a new subgenus of Chalicodoma from Southeast Asia (Hymenoptera, Megachilidae)

    Science.gov (United States)

    Engel, Michael S.; Gonzalez, Victor H.

    2011-01-01

    Abstract A new subgenus, Alocanthedon Engel and Gonzalez subgen. n., is described for five species of unusual Southeast Asian bees in the genus Chalicodoma Lepeletier de Saint Fargeau (Megachilinae: Megachilini). The subgenus is most noteworthy for the deep postgenal depression or furrow in males (bordered outwardly near the base of the mandible by a protuberant, thick lamella) and the presence of a dense patch of black setae posteriorly in the forewing medial cell (except in one species) [resembling the dense patch of setae among the submarginal cells of Thrinchostoma Saussure (Halictidae: Halictinae: Halictini)]. The subgenus is characterized and distinguished from the related Callomegachile Michener. A key to the following five species presently included in the subgenus is provided: Chalicodoma aterrimum (Smith), Chalicodoma atratiforme (Meade-Waldo) comb. n., Chalicodoma memecylonae Engel sp. n., Chalicodoma odontophorum Engel sp. n., and Chalicodoma apoicola Engel sp. n. Chalicodoma (Callomegachile) atratiforme sininsulae (Cockerell) is newly placed in synonymy with C. (C.) fulvipenne (Smith). Species have been collected from Memecylaceae (Myrtales) and Fabaceae (Fabales). The phylogenetic relationships of Alocanthedon among other Megachilini are briefly elaborated upon. PMID:21747670

  17. Flight activity and responses to climatic conditions of two subspecies of Melipona marginata Lepeletier (Apidae, Meliponinae)

    Energy Technology Data Exchange (ETDEWEB)

    Kleinert-Giovannini, A; Imperatriz-Fonseca, V L

    1986-01-01

    The flight activity of two colonies of M. m. marginata and six colonies of M. m. obscurior was studied in Sao Paulo, Brazil during three periods in 1981-1983. All colonies were hived except for one colony of M. m. obscurior which nested in a hollow tree. The two subspecies showed the same responses to the climatic factors analyzed. Flight activity was correlated positively with temperature and negatively with RH. In favorable conditions foraging took place throughout the day but both species were most active between 11 and 13 h. In the last observation period (October 1982-January 1983), when only two obscurior colonies were studied, flight activity was not correlated to RH, occurring even when RH was unfavorably high. This was a very rainy season, and it is suggested that the behavior of the bees indicates a flexible response to prevailing weather conditions.

  18. De roodrandzandbij Andrena rosae in de Zuid-Hollandse Biesbosch (Hymenoptera: Apoidea: Andrenidae).

    NARCIS (Netherlands)

    Meer, van der F.; Reemer, M.; Peeters, T.M.J.

    2006-01-01

    Andrena rosae in the Zuid-Hollandse Biesbosch (Hymenoptera: Apoidea: Andrenidae) Andrena rosae is a fairly large, black and red mining bee. It has declined dramatically in the 20th century in northwestern Europe. In the Netherlands the species only survived in and around the Biesbosch, at the

  19. Primera cita de Aleiodes laphygmae (Hymenoptera: Braconidae para Argentina y de su asociación con larvas de Spodoptera eridania (Lepidoptera: Noctuidae First record of Aleiodes laphygmae (Hymenoptera: Braconidae for Argentina and its association with larvae of Spodoptera eridania (Lepidoptera: Noctuidae

    Directory of Open Access Journals (Sweden)

    Liliana Valverde

    2012-06-01

    Full Text Available Se reporta por primera vez para Argentina Aleiodes laphygmae (Viereck (Hymenoptera: Braconidae y como parasitoide de larvas de Spodoptera eridania (Stoll (Lepidoptera: Noctuidae, en plantaciones de soja en la provincia de Tucumán (Argentina. Se provee información biológica como hábitos, hospedadores y distribución.Aleiodes laphygmae (Viereck (Hymenoptera: Braconidae, is reported for the first time for Argentina. It is also reported parasitizing larvae of Spodoptera eridania (Stoll (Lepidoptera: Noctuidae in soybeans crops in Tucumán province (Argentina. Biological information on habits, hosts and distribution is provided.

  20. An epidemiological survey of hymenoptera venom allergy in the Spanish paediatric population.

    Science.gov (United States)

    Martínez-Cañavate, A; Tabar, A I; Eseverri, J L; Martín, F; Pedemonte-Marco, C

    2010-01-01

    Hypersensitivity reactions to hymenoptera venom are infrequent in paediatric patients. A study was made to determine the incidence of this pathology in children, based on an epidemiological survey targeted to all members of the SEICAP (Sociedad Española de Inmunología Clínica y Alergia Pediátrica/Spanish Society of Paediatric Clinical Immunology and Allergy), and designed to collect the data on patients under 17 years of age diagnosed with hymenoptera venom allergy. The data corresponding to 175 patients (135 males) were collected. The mean age was 9.9 ± 3.6 years. Seventeen percent (32 patients) were the offspring of beekeepers, and 68.9% had experienced previous stings. The causal insect was Apis melifera, implicated in 55 cases, followed by Polistes dominulus (33 cases). In 151 patients (83.9%) the condition consisted of a local reaction. The most frequent systemic response was urticaria and angio-oedema. Fourteen patients suffered anaphylactic shock. The diagnosis was based on skin test (intradermal and prick) and/or specific IgE testing. Three treatment categories were established: (a) prevention and educational measures; (b) symptomatic treatment with oral antihistamines as well as self-injectable adrenalin; and (c) immunotherapy. In this context, 135 patients underwent immunotherapy with a mean duration of 3.5 ± 1.7 years (range 2-5 years) - with excellent tolerance. The starting regimen was predominantly conventional (92 patients). The results of this survey show hypersensitivity reactions to hymenoptera venom to be infrequent in paediatrics, though with a strong impact upon patient quality of life. Copyright © 2009 SEICAP. Published by Elsevier Espana. All rights reserved.

  1. Risk of anaphylaxis in patients with large local reactions to hymenoptera stings: a retrospective and prospective study.

    Science.gov (United States)

    Pucci, Stefano; D'Alò, Simona; De Pasquale, Tiziana; Illuminati, Ilenia; Makri, Elena; Incorvaia, Cristoforo

    2015-01-01

    In the few studies available, the risk of developing systemic reactions (SR) to hymenoptera stings in patients with previous large local reactions (LLRs) to stings ranges from 0 to 7 %. We evaluated both retrospectively and prospectively the risk of SRs in patients with LLRs to stings. An overall number of 477 patients, 396 with an SR as the first manifestation of allergy and 81 with a history of only LLRs after hymenoptera stings, were included in the study. All patients had clinical history and allergy testing (skin tests and/or specific IgE) indicative of allergy to venom of only one kind of Hymenoptera. Of the 81 patient with LLRs, 53 were followed-up for 3 years by annual control visits, while the 396 patients with SR were evaluated retrospectively. Among the 396 patients with an SR, only 17 (4.2 %) had had a previous LLR as debut of allergy, after an history of normal local reactions to Hymenoptera stings. All the 81 patients with a history of only LLRs had previously had at least two LLRs, with an overall number of 238 stings and no SR. Among the 53 patients who were prospectively evaluated we found that 31 of them (58.3 %) were restung by the same type of insect, with an overall number of 59 stings, presenting only LLRs and no SR. Our findings confirm that patients with repeated LLRs to stings had no risk of SR, while a single LLR does not exclude such risk. This has to be considered in the management of patients with LLRs.

  2. Parasitismo entre especies (Diptera, Hymenoptera en los nidos de Stictia signata (Hymenoptera: Sphecidae

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    Julio A. Genaro

    1999-09-01

    Full Text Available S. signata es una de las avispas de la arena más frecuentemente observada en los cayos y las costas de Cuba. Las hembras construyen los nidos en la arena y los abastecen con moscas, para alimentar a la descendencia. Se describe la conducta de dos especies: Liohippelates n. sp. circa collusor (Diptera: Chloropidae y Hexacola sp. (Hymenoptera: Eucoilidae para penetrar al interior de los nidos de S. signata. Las observaciones se efectuaron durante 1989 hasta 1991, en playa Caimito, sur de la provincia de La Habana, Cuba. Liohippelates cleptoparasitó el 100% de los nidos. Sus larvas necrófagas se alimentaron de los restos de las presas dejadas por la larva de S. signata, sin afectarla. Sólo en un caso la larva mostró signos de mortalidad, porque además del número alto de cleptoparásitos inmaduros, habían 53 moscas adultas alimentándose de los fluidos corporales de las presas. Hexacola sp. fue un parasitoide de las larvas de Liohippelates, en el interior de las celdillas. A pesar del elevado cleptoparasitismo, la población del esfécido se mantuvo elevada durante los años de observación.Stictia signata is one of the most frequently observed sand wasps in the Cuban keys and coasts. Females build their nests in the sand and supply them with flies to feed offspring. Here, I describe the behavior of two species, Liohippelates n. sp. near collusor (Diptera: Chloropidae and Hexacola sp. (Hymenoptera: Eucoilidae, which enter the nests of S. signata. The observations were carried out from 1989 through 1991 in Caimito beach, Southern Havana province, Cuba. Liohippelates inhabited 100% of the nests. Its necrofagous larvae fed on the remnants of prey left by the larva of S. signata, without affecting the larva. Only in one case did the larva show signs of mortality because, apart from the high number of immature cleptoparasites, there were 53 adult flies feeding on prey body fluids. Hexacola sp. parasitized the larvae of Liohippelates within the

  3. Famílias de Hymenoptera (Insecta como ferramenta avaliadora da conservação de restingas no extremo sul do Brasil

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    Cristina Maria Loyola Zardo

    2009-12-01

    Full Text Available Com o objetivo de estimar e comparar a diversidade e flutuação populacional das famílias de Hymenoptera em área de restinga foi realizado um levantamento faunístico em duas áreas de restinga com diferentes níveis de conservação. Foram coletados 5.518 himenópteros distribuídos em 30 famílias. Os picos populacionais na flutuação das famílias ocorreram no verão confirmando a alta correlação da temperatura com a distribuição das famílias. Constatou-se na restinga em sucessão maior riqueza, porém, com alta dominância, abrigando representantes dos três grupos ecológicos (antófilos, generalistas e parasitóides em alta abundância. A restinga preservada, com 17 famílias, verificou-se mais diversa e homogênea, onde verificou-se maior abundância dos parasitóides, devido à maior estabilidade do sistema. A riqueza de famílias de Hymenoptera em áreas de restinga pode ser utilizada como parâmetro indicativo de qualidade ambiental, para este tipo de bioma.Hymenoptera Families (Insecta as Evaluation Tool of the Conservation of Sandbanks in Southern BrazilAbstract. With aim to estimate and compare the diversity and population of the Hymenoptera families in a sandbank area was carried out a wildlife survey in two areas of sandbank with different levels of conservation. We collected 5 518 Hymenoptera distributed in 30 families. The peaks in the families fluctuation occurred in the summer confirmed the high correlation of temperature with the distribution of families. The sandbank in succession had the highest richness, however with high dominance, hosting representatives of the three ecological groups (anthophilous, generalists and parasitoids in high abundance. The sandbank preserved, with 17 families, was more diverse and homogeneous, where the parasitoids showed greater abundance due to greater system stability. The richness of Hymenoptera families in sandbanks can be used as a parameter indicative of environmental quality.

  4. The invasion, provenance and diversity of Vespa velutina Lepeletier (Hymenoptera: Vespidae in Great Britain.

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    Giles E Budge

    Full Text Available The yellow-legged or Asian hornet (Vespa velutina colour form nigrithorax was introduced into France from China over a decade ago. Vespa velutina has since spread rapidly across Europe, facilitated by suitable climatic conditions and the ability of a single nest to disperse many mated queens over a large area. Yellow-legged hornets are a major concern because of the potential impact they have on populations of many beneficial pollinators, most notably the western honey bee (Apis mellifera, which shows no effective defensive behaviours against this exotic predator. Here, we present the first report of this species in Great Britain. Actively foraging hornets were detected at two locations, the first around a single nest in Gloucestershire, and the second a single hornet trapped 54 km away in Somerset. The foraging activity observed in Gloucestershire was largely restricted to within 700 m of a single nest, suggesting highly localised movements. Genetic analyses of individuals from the Gloucestershire nest and the single hornet from Somerset suggest that these incursions represent an expansion of the European population, rather than a second incursion from Asia. The founding queen of the Gloucestershire nest mated with a single male, suggesting that sexual reproduction may have occurred in an area of low nest density. Whilst the nest contained diploid adult males, haploid 'true' males were only present at the egg stage, indicating that the nest was detected and removed before the production of queens. Members of the public reported additional dead hornets associated with camping equipment recently returned from France and imported timber products, highlighting possible pathways of incursion. The utility of microsatellites to inform surveillance during an incursion and the challenge of achieving eradication of this damaging pest are discussed.

  5. Application of recombinant antigen 5 allergens from seven allergy-relevant Hymenoptera species in diagnostics

    DEFF Research Database (Denmark)

    Schiener, Maximilian; Eberlein, Bernadette; Moreno Aguilar, Carmen

    2017-01-01

    BACKGROUND: Hymenoptera stings can cause severe anaphylaxis in untreated venom-allergic patients. A correct diagnosis regarding the relevant species for immunotherapy is often hampered by clinically irrelevant cross-reactivity. In vespid venom allergy, cross-reactivity between venoms of different...

  6. Accelerated evolution of mitochondrial but not nuclear genomes of Hymenoptera: new evidence from crabronid wasps.

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    Martin Kaltenpoth

    Full Text Available Mitochondrial genes in animals are especially useful as molecular markers for the reconstruction of phylogenies among closely related taxa, due to the generally high substitution rates. Several insect orders, notably Hymenoptera and Phthiraptera, show exceptionally high rates of mitochondrial molecular evolution, which has been attributed to the parasitic lifestyle of current or ancestral members of these taxa. Parasitism has been hypothesized to entail frequent population bottlenecks that increase rates of molecular evolution by reducing the efficiency of purifying selection. This effect should result in elevated substitution rates of both nuclear and mitochondrial genes, but to date no extensive comparative study has tested this hypothesis in insects. Here we report the mitochondrial genome of a crabronid wasp, the European beewolf (Philanthus triangulum, Hymenoptera, Crabronidae, and we use it to compare evolutionary rates among the four largest holometabolous insect orders (Coleoptera, Diptera, Hymenoptera, Lepidoptera based on phylogenies reconstructed with whole mitochondrial genomes as well as four single-copy nuclear genes (18S rRNA, arginine kinase, wingless, phosphoenolpyruvate carboxykinase. The mt-genome of P. triangulum is 16,029 bp in size with a mean A+T content of 83.6%, and it encodes the 37 genes typically found in arthropod mt genomes (13 protein-coding, 22 tRNA, and two rRNA genes. Five translocations of tRNA genes were discovered relative to the putative ancestral genome arrangement in insects, and the unusual start codon TTG was predicted for cox2. Phylogenetic analyses revealed significantly longer branches leading to the apocritan Hymenoptera as well as the Orussoidea, to a lesser extent the Cephoidea, and, possibly, the Tenthredinoidea than any of the other holometabolous insect orders for all mitochondrial but none of the four nuclear genes tested. Thus, our results suggest that the ancestral parasitic lifestyle of

  7. The species of the Neotropical genus Fractipons Townes, 1970 (Hymenoptera, Ichneumonidae, Cryptinae).

    Science.gov (United States)

    Bordera, Santiago; González-Moreno, Alejandra

    2011-01-19

    In this paper, two new species of the Neotropical genus Fractipons Townes, 1970 (Hymenoptera, Ichneumonidae) are described. A new diagnosis for the genus, a re-description of Fractipons cincticornis Townes, 1970 and a key to known species are provided. New distribution records for the genus now include Argentina, Costa Rica, Panama and Peru.

  8. Two new species of the genus Peristenus Foerster (Hymenoptera: Braconidae: Euphorinae) from the Canary Islands

    NARCIS (Netherlands)

    Achterberg, van C.; Guerrero, E.R.

    2003-01-01

    Two new species of the genus Peristenus Foerster (Hymenoptera: Braconidae: Euphorinae) from the Canary Islands are described and illustrated: Peristenus angifemoralis spec. nov. from Tenerife, and P. gloriae spec. nov. from Gran Canaria and Tenerife.

  9. Single locus complementary sex determination in Hymenoptera: an "unintelligent" design?

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    Driessen Gerard

    2006-01-01

    Full Text Available Abstract The haplodiploid sex determining mechanism in Hymenoptera (males are haploid, females are diploid has played an important role in the evolution of this insect order. In Hymenoptera sex is usually determined by a single locus, heterozygotes are female and hemizygotes are male. Under inbreeding, homozygous diploid and sterile males occur which form a genetic burden for a population. We review life history and genetical traits that may overcome the disadvantages of single locus complementary sex determination (sl-CSD. Behavioural adaptations to avoid matings between relatives include active dispersal from natal patches and mating preferences for non-relatives. In non-social species, temporal and spatial segregation of male and female offspring reduces the burden of sl-CSD. In social species, diploid males are produced at the expense of workers and female reproductives. In some social species, diploid males and diploid male producing queens are killed by workers. Diploid male production may have played a role in the evolution or maintenance of polygyny (multiple queens and polyandry (multiple mating. Some forms of thelytoky (parthenogenetic female production increase homozygosity and are therefore incompatible with sl-CSD. We discuss a number of hypothetical adaptations to sl-CSD which should be considered in future studies of this insect order.

  10. Notas sobre a bionomia de Trichotrigona extranea Camargo & Moure (Hymenoptera, Apidae, Meliponini Notes on the bionomy of Trichotrigona extranea Camargo & Moure (Hymenoptera, Apidae, Meliponini

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    João M. F. Camargo

    2007-03-01

    Full Text Available Três ninhos de T. extranea, encontrados nas matas de igapó, na localidade de Samaúma, foz do rio Daraã (64°45'35" W, 0°27'7" S, AM, Brasil, são descritos e ilustrados. Este é o único lugar onde esta espécie é conhecida. Todos os ninhos estavam em ocos de ramos secos de "Tanimbuca" - Buchenavia suaveolens Eichler (Combretaceae. Dois deles compartilhavam o oco com ninhos de Frieseomelitta sp. e um era solitário. Um ninho continha 46 células de cria, 43 operárias adultas e uma rainha fisogástrica; o segundo, 240 células, 117 operárias, 25 machos e uma rainha fisogástrica; o terceiro, 260 células, 163 operárias, uma rainha fisogástrica e uma pupa de rainha. As células são construídas na forma de cacho, como em Friseomelitta, e as células de rainha são do mesmo tamanho e forma que as de operária. Não há potes de alimento - este é o único Meliponini que se conhece que não armazena alimento. Supostamente, esta espécie tem hábitos cleptobióticos. Rainhas e machos são também descritos.Three nests of T. extranea, found in "igapó" forests in the locality of Samaúma, mouth of Daraã river (64°45'35" W, 0°27'7" S, AM, Brazil, are described and illustrated. This is the only place from where this species is known. All nests were located in hollow of dried branches of "Tanimbuca" - Buchenavia suaveolens Eichler (Combretaceae. Two of them were sharing the hollow with nests of Frieseomelitta sp. and one was solitary. One nest had 46 brood cells, 43 adult workers and one physogastric queen; the second one, 240 cells, 117 adult workers, 25 males and one physogastric queen; the third one, 260 cells, 163 workers, one physogastric queen and one queen pupa. The cells are arranged in clusters, like in Frieseomelitta, and the queen cells has the same shape and size as the worker ones. There are no food pots - this is the only known Meliponini that does not store food. Supposedly, this species is cleptobiotic. Queens and males are also described.

  11. A NEW SPECIES OF INVASIVE GALL WASP (HYMENOPTERA: EULOPHIDAE: TETRASTICHINAE) ON BLUE GUM (EUCALYPTUS GLOBULUS) IN CALIFORNIA

    Science.gov (United States)

    The blue gum gall wasp, Selitrichodes globulus La Salle & Gates (Hymenoptera: Eulophidae: Tetrastichinae), is described as an invasive gall inducer on blue gum, Eucalyptus globulus (Myrtaceae), in California....

  12. A Gynandromorph and Teratological Case in Spilomicrus sp. (Hymenoptera, Diaprioidea, Diapriidae

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    Emerson Comério

    2015-12-01

    Resumo. Este estudo relata a ocorrência de antenas com características femininas e masculinas em um exemplar fêmea de Spilomicrus sp. (Hymenoptera, Diaprioidea, Diapriidae coletado no Parque Estadual Intervales, Ribeirão Grande, São Paulo, Brasil, assim como malformações em uma das antenas, que são aqui descritas e ilustradas.

  13. PERBANDINGAN KEANEKARAGAMAN HYMENOPTERA PARASITOID PADA AGROEKOSISTEM KEDELAI DENGAN APLIKASI DAN TANPA APLIKASI INSEKTISIDA

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    Hendrival Hendrival

    2017-04-01

    Full Text Available Abstrak Sistem pengelolaan tanaman kedelai dengan penggunaan insektisida sintetik yang intensif akan menurunkan keanekaragaman jenis Hymenoptera parasitoid. Penelitian bertujuan untuk memban-dingkan keanekaragaman Hymenoptera parasitoid pada agroekosistem kedelai dengan dan tanpa aplikasi insektisida sintetik. Pengumpulan data serangga menggunakan perangkap dari jaring serangga dan nampan kuning. Hasil penelitian menunjukkan bahwa indeks keanekaragaman jenis pada fase pertumbuhan vegetatif dan generatif kedelai dengan aplikasi insektisida lebih rendah dibandingkan dengan yang tanpa aplikasi insektisida, yang keduanya tergolong sedang. Indeks kemerataan jenis pada fase pertumbuhan vegetatif dan generatif dari kedua agroekosistem kedelai tergolong tinggi. Indeks kekayaan jenis pada fase vegetatif dari agroekosistem kedelai dengan aplikasi insektisida tergolong rendah (0<2,3955≤2,5, sedangkan pada fase generatif tergolong sedang (0<3,6118≤4. Indeks kekayaan jenis pada fase vegetatif (0<2,6229≤4 dan generatif (0<3,8287≤4 dari agroekosistem kedelai tanpa aplikasi insektisida tergolong sedang. Komunitas Hymenoptera parasitoid pada agroekosistem kedelai tanpa aplikasi insektisida memiliki kemiripan lebih rendah daripada yang dengan aplikasi insektisida. Aplikasi insektisida mempengaruhi indeks kekayaan jenis pada fase generatif dan kemiripan komunitasnya, yaitu nilainya lebih rendah daripada yang tanpa insektisida. Abstract The management system of soybean agroecosystem with an intensive use of synthetic insecticides will reduce the diversity of parasitoid Hymenoptera species. The study aimed to compare the diversity of the parasitoids in soybean agroecosystem with and without insecticide application. The collection of the parasitoid used insect net and yellow tray. The results showed that the diversity index of the parasitoids during vegetative and generative growth of the soybean with the insecticide application was lower than the one without

  14. Comparative mitogenomics of Braconidae (Insecta: Hymenoptera) and the phylogenetic utility of mitochondrial genomes with special reference to Holometabolous insects

    Science.gov (United States)

    2010-01-01

    Background Animal mitochondrial genomes are potential models for molecular evolution and markers for phylogenetic and population studies. Previous research has shown interesting features in hymenopteran mitochondrial genomes. Here, we conducted a comparative study of mitochondrial genomes of the family Braconidae, one of the largest families of Hymenoptera, and assessed the utility of mitochondrial genomic data for phylogenetic inference at three different hierarchical levels, i.e., Braconidae, Hymenoptera, and Holometabola. Results Seven mitochondrial genomes from seven subfamilies of Braconidae were sequenced. Three of the four sequenced A+T-rich regions are shown to be inverted. Furthermore, all species showed reversal of strand asymmetry, suggesting that inversion of the A+T-rich region might be a synapomorphy of the Braconidae. Gene rearrangement events occurred in all braconid species, but gene rearrangement rates were not taxonomically correlated. Most rearranged genes were tRNAs, except those of Cotesia vestalis, in which 13 protein-coding genes and 14 tRNA genes changed positions or/and directions through three kinds of gene rearrangement events. Remote inversion is posited to be the result of two independent recombination events. Evolutionary rates were lower in species of the cyclostome group than those of noncyclostomes. Phylogenetic analyses based on complete mitochondrial genomes and secondary structure of rrnS supported a sister-group relationship between Aphidiinae and cyclostomes. Many well accepted relationships within Hymenoptera, such as paraphyly of Symphyta and Evaniomorpha, a sister-group relationship between Orussoidea and Apocrita, and monophyly of Proctotrupomorpha, Ichneumonoidea and Aculeata were robustly confirmed. New hypotheses, such as a sister-group relationship between Evanioidea and Aculeata, were generated. Among holometabolous insects, Hymenoptera was shown to be the sister to all other orders. Mecoptera was recovered as the

  15. Comparative mitogenomics of Braconidae (Insecta: Hymenoptera and the phylogenetic utility of mitochondrial genomes with special reference to Holometabolous insects

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    Shi Min

    2010-06-01

    Full Text Available Abstract Background Animal mitochondrial genomes are potential models for molecular evolution and markers for phylogenetic and population studies. Previous research has shown interesting features in hymenopteran mitochondrial genomes. Here, we conducted a comparative study of mitochondrial genomes of the family Braconidae, one of the largest families of Hymenoptera, and assessed the utility of mitochondrial genomic data for phylogenetic inference at three different hierarchical levels, i.e., Braconidae, Hymenoptera, and Holometabola. Results Seven mitochondrial genomes from seven subfamilies of Braconidae were sequenced. Three of the four sequenced A+T-rich regions are shown to be inverted. Furthermore, all species showed reversal of strand asymmetry, suggesting that inversion of the A+T-rich region might be a synapomorphy of the Braconidae. Gene rearrangement events occurred in all braconid species, but gene rearrangement rates were not taxonomically correlated. Most rearranged genes were tRNAs, except those of Cotesia vestalis, in which 13 protein-coding genes and 14 tRNA genes changed positions or/and directions through three kinds of gene rearrangement events. Remote inversion is posited to be the result of two independent recombination events. Evolutionary rates were lower in species of the cyclostome group than those of noncyclostomes. Phylogenetic analyses based on complete mitochondrial genomes and secondary structure of rrnS supported a sister-group relationship between Aphidiinae and cyclostomes. Many well accepted relationships within Hymenoptera, such as paraphyly of Symphyta and Evaniomorpha, a sister-group relationship between Orussoidea and Apocrita, and monophyly of Proctotrupomorpha, Ichneumonoidea and Aculeata were robustly confirmed. New hypotheses, such as a sister-group relationship between Evanioidea and Aculeata, were generated. Among holometabolous insects, Hymenoptera was shown to be the sister to all other orders

  16. Revision of the subfamily Euphorinae (excluding the tribe Meteorini Cresson) (Hymenoptera: Braconidae) from China

    NARCIS (Netherlands)

    Chen, X.; Achterberg, van C.

    1997-01-01

    The subfamily Euphorinae (excluding the tribe Meteorini Cresson) (Hymenoptera: Braconidae) from China is revised. In total 150 species, belonging to 24 genera, are treated and keyed. One genus (Heia gen. nov.; type species: Heia robustipes spec. nov.) and 69 species are described as new to science.

  17. Prey identification in nests of the potter wasp Hypodynerus andeus (Packard (Hymenoptera, Vespidae, Eumeninae using DNA barcodes

    Directory of Open Access Journals (Sweden)

    Héctor A. Vargas

    2014-06-01

    Full Text Available Prey identification in nests of the potter wasp Hypodynerus andeus (Packard (Hymenoptera, Vespidae, Eumeninae using DNA barcodes. Geometrid larvae are the only prey known for larvae of the Neotropical potter wasp Hypodynerus andeus (Packard, 1869 (Hymenoptera, Vespidae, Eumeninae in the coastal valleys of the northern Chilean Atacama Desert. A fragment of the mitochondrial gene cytochrome oxidase c subunit 1 was amplified from geometrid larvae collected from cells of H. andeus in the Azapa Valley, Arica Province, and used to provide taxonomic identifications. Two species, Iridopsis hausmanni Vargas, 2007 and Macaria mirthae Vargas, Parra & Hausmann, 2005 were identified, while three others could be identified only at higher taxonomic levels, because the barcode reference library of geometrid moths is still incomplete for northern Chile.

  18. DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini)

    OpenAIRE

    Rocha, MP; Pompolo, SD; Dergam, JA; Fernandes, A; Campos, LAD

    2002-01-01

    We analyzed patterns of heterochromatic bands in the Neotropical stingless bee genus Melipona (Hymenoptera, Meliponini). Group I species (Melipona bicolor bicolor, Melipona quadrifasciata, Melipona asilvae, Melipona marginata, Melipona subnitida) were characterized by low heterochromatic content. Group 11 species (Melipona capixaba, Melipona compressipes, Melipona crinita, Melipona seminigra fuscopilosa e Melipona scutellaris) had high heterochromatic content. All species had 2n = 18 and n = ...

  19. Diploid Male Production of Two Amazonian Melipona Bees (Hymenoptera: Apidae

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    Izaura Bezerra Francini

    2012-01-01

    Full Text Available The diploid male has already been recorded for Melipona Illger, and herein, in Melipona seminigra merrillae Cockerell and Melipona interrupta manaosensis Schwarz. This paper was carried out at the Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, AM, Brazil. We produced and monitored 31 new colonies of M. s. merrillae and 32 new colonies of M. i. manaosensis. We sampled 2,995 pupae of M. s. merrillae and 2,020 of M. i. manaosensis. In colonies with a 1 : 1 sex ratio, male diploidy was confirmed by cytogenetic analysis and workers’ behavior. We estimated 16 sex-determining alleles in M. s. merrillae and 22 in M. i. manaosensis. In colonies of M. i. manaosensis in a 1 : 1 sex ratio, workers killed the males and the queen that produced them soon after they emerged, as predicted. This behavior was not registered for M. s. merrillae, and sex ratios did not stay 1 : 1, indicating polyandry for this species.

  20. Microsatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae).

    Science.gov (United States)

    Lopes, Denilce Meneses; D Silva, Filipe Oliveira; Fernandes Salomão, Tânia Maria; Campos, Lúcio Antônio D Oliveira; Tavares, Mara Garcia

    2009-05-01

    Eight microsatellite primers were developed from ISSR (intersimple sequence repeats) markers for the stingless bee Melipona rufiventris. These primers were tested in 20 M. rufiventris workers, representing a single population from Minas Gerais state. The number of alleles per locus ranged from 2 to 5 (mean = 2.63) and the observed and expected heterozygosity values ranged from 0.00 to 0.44 (mean = 0.20) and from 0.05 to 0.68 (mean = 0.31), respectively. Several loci were also polymorphic in M. quadrifasciata, M. bicolor, M. mandacaia and Partamona helleri and should prove useful in population studies of other stingless bees. © 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd.

  1. Photoreceptor spectral sensitivity in the bumblebee, Bombus impatiens (Hymenoptera: Apidae.

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    Peter Skorupski

    Full Text Available The bumblebee Bombus impatiens is increasingly used as a model in comparative studies of colour vision, or in behavioural studies relying on perceptual discrimination of colour. However, full spectral sensitivity data on the photoreceptor inputs underlying colour vision are not available for B. impatiens. Since most known bee species are trichromatic, with photoreceptor spectral sensitivity peaks in the UV, blue and green regions of the spectrum, data from a related species, where spectral sensitivity measurements have been made, are often applied to B impatiens. Nevertheless, species differences in spectral tuning of equivalent photoreceptor classes may result in peaks that differ by several nm, which may have small but significant effects on colour discrimination ability. We therefore used intracellular recording to measure photoreceptor spectral sensitivity in B. impatiens. Spectral peaks were estimated at 347, 424 and 539 nm for UV, blue and green receptors, respectively, suggesting that this species is a UV-blue-green trichromat. Photoreceptor spectral sensitivity peaks are similar to previous measurements from Bombus terrestris, although there is a significant difference in the peak sensitivity of the blue receptor, which is shifted in the short wave direction by 12-13 nm in B. impatiens compared to B. terrestris.

  2. An Early Miocene bumble bee from northern Bohemia (Hymenoptera, Apidae

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    Jakub Prokop

    2017-10-01

    Full Text Available A new species of fossil bumble bee (Apinae: Bombini is described and figured from Early Miocene (Burdigalian deposits of the Most Basin at the Bílina Mine, Czech Republic. Bombus trophonius sp. n., is placed within the subgenus Cullumanobombus Vogt and distinguished from the several species groups therein. The species is apparently most similar to the Nearctic B. (Cullumanobombus rufocinctus Cresson, the earliest-diverging species within the clade and the two may be related only by symplesiomorphies. The age of the fossil is in rough accordance with divergence estimations for Cullumanobombus.

  3. A new subgenus of Heterotrigona from New Guinea (Hymenoptera: Apidae)

    DEFF Research Database (Denmark)

    Engel, Michael S.; Rasmussen, Claus

    2017-01-01

    A new subgenus is established within the Indomalayan stingless bee genus Heterotrigona Schwarz (Meliponini). Sahulotrigona Engel & Rasmussen, new subgenus, is distinguished from amongst other Heterotrigona, particularly the subgenus Platytrigona Moure, within which one of the two included species...... in the following new combinations: Heterotrigona (Platytrigona) flaviventris (Friese), H. (P.) hobbyi (Schwarz), H. (P.) keyensis (Friese), H. (P.) lamingtonia (Cockerell), H. (P.) planifrons (Smith), H. (Sundatrigona) lieftincki (Sakagami & Inoue), and H. (Su.) moorei (Schwarz). The stingless bees of Papuasia...

  4. Note on glands present in meliponinae (Hymenoptera, Apidae bees legs

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    Carminda da Cruz-Landim

    1998-01-01

    Full Text Available The present paper reports the presence of glandular structures in legs of some stingless bee species. The glands appear as: the epidermis transformation in a glandular epithelium as in basitarsus, an epithelial sac inside the segment as in the femur of queens or in the last tarsomere, as round glandular cells, scattered or forming groupments. The saculiform gland of femur is present only in queens, the other glands are present in males, queens and workers of the studied species, apparently without any type of polymorphism. This occurrence seems indicate that the function of these glands have not to do with the sociality or specific behavior of castes.

  5. Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)

    OpenAIRE

    Waldschmidt, Ana Maria; Salomão, Tânia Maria Fernandes; Barros, Everaldo Gonçalves de; Campos, Lúcio de Antônio Oliveira

    1997-01-01

    The objective of the present study was to test three different procedures for DNA extraction of Melipona quadrifasciata based on existing methods for DNA extraction of Apis, plants and fungi. These methods differ in the concentrations of specific substances in the extraction buffer. The results demonstrate that the method used for Apis is not adequate for DNA extraction from M. quadrifasciata. On the other hand, with minor modifications this method and the methods for plants and fungi were ad...

  6. An overview of cytogenetics of the tribe Meliponini (Hymenoptera: Apidae).

    Science.gov (United States)

    Tavares, Mara Garcia; Lopes, Denilce Meneses; Campos, L A O

    2017-06-01

    The present study provides a comprehensive review of cytogenetic data on Meliponini and their chromosomal evolution. The compiled data show that only 104 species of stingless bees, representing 32 of the 54 living genera have been studied cytogenetically and that among these species, it is possible to recognize three main groups with n = 9, 15 and 17, respectively. The first group comprises the species of the genus Melipona, whereas karyotypes with n = 15 and n = 17 have been detected in species from different genera. Karyotypes with n = 17 are the most common among the Meliponini studied to date. Cytogenetic information on Meliponini also shows that although chromosome number, in general, is conserved among species of a certain genus, other aspects, such as chromosome morphology, quantity, distribution and composition of heterochromatin, may vary between them. This reinforces the fact that the variations observed in the karyotypes of different Meliponini groups cannot be explained by a single theory or a single type of structural change. In addition, we present a discussion about how these karyotype variations are related to the phylogenetic relationships among the different genera of this tribe.

  7. Observations on fragrance collection behaviour of euglossine bees (Hymenoptera, Apidae

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    Peter W.H. Holland

    2015-03-01

    Full Text Available Male bees of the tribe Euglossini collect volatile chemicals secreted by orchids using dense patches of hair on the front tarsi. After collecting chemicals, the bee hovers while transferring these fragrances to invaginations on the hind tibiae. The fragrance collection and hovering behaviours are repeated multiple times. Here I report preliminary field observations on the length of fragrance collection and hovering phases in bees of the Eulaema meriana (Oliver, 1789 mimicry complex visiting the orchid Catasetum discolor in Kavanayén, Venezuela. I observed that in extended visits with many cycles of fragrance collection and hovering, the length of each collection phase gradually increased, while the length of hovering phase was static. This suggests either that chemicals secreted by orchids are in limited supply or that efficiency of fragrance collection drops.

  8. New species of the stingless bee genus Schwarziana (Hymenoptera, Apidae

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    Gabriel A.R. Melo

    2015-12-01

    Full Text Available ABSTRACT Two new species of the stingless bee genus Schwarziana from Brazil are described and illustrated. Schwarziana bocainensis sp. nov. is described from Serra da Bocaina, in São Paulo, and S. chapadensis sp. nov. is described from Chapada dos Veadeiros, in Goiás. An identification key to workers of the known species of Schwarziana is provided.

  9. Sex Determination in Bees. IV. Genetic Control of Juvenile Hormone Production in MELIPONA QUADRIFASCIATA (Apidae)

    Science.gov (United States)

    Kerr, Warwick Estevam; Akahira, Yukio; Camargo, Conceição A.

    1975-01-01

    Cell number and volume of corpora allata was determined for 8 phases of development, the first prepupal stage to adults 30 days old, in the social Apidae Melipona quadrifasciata. In the second prepupal stage a strong correlation was found between cell number and body weight ( r=0.651**), and cell number and corpora allata volume in prepupal stage (r=0.535*), which indicates that juvenile hormone has a definite role in caste determination in Melipona. The distribution of the volume of corpus allatum suggest a 3:1 segregation between bees with high volume of corpora allata against low and medium volume. This implies that genes xa and xb code for an enzyme that directly participates in juvenile hormone production. It was also concluded that the number of cells in the second prepupal stage is more important than the weight of the prepupa for caste determination. A scheme summarizing the genic control of sex and caste determination in Melipona bees in the prepupal phase is given. PMID:1213273

  10. Preliminary taxonomic study of the genus Praon (Hymenoptera:Braconidae:Aphidiinae) and its host associations in Iran

    Czech Academy of Sciences Publication Activity Database

    Rakhshani, E.; Talebi, A. A.; Manzari, S.; Tomanovic, Ž.; Starý, Petr; Rezwani, A.

    2007-01-01

    Roč. 26, č. 2 (2007), s. 19-34 ISSN 0259-9996 R&D Projects: GA AV ČR IBS5007102 Institutional research plan: CEZ:AV0Z50070508 Keywords : genus Praon * Hymenoptera * Iran Subject RIV: EH - Ecology, Behaviour

  11. Impact of Hymenoptera venom allergy and the effects of specific venom immunotherapy on mast cell metabolites in sensitized children

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    Ewa Cichocka-Jarosz

    2014-06-01

    Full Text Available introduction and objective. Mast cells (MC are effector cells during severe systemic reactions (SR to Hymenoptera stings. Venom specific immunotherapy (VIT is the treatment of choice for prevention of SR to stings. Tryptase and prostaglandin D[sub]2[/sub] metabolites (PGD[sub]2[/sub] are the markers of MC activation. The study design was to 1. compare baseline values of serum tryptase concentration (BST and PGD[sub]2[/sub] metabolites in children with/without venom sensitization, 2. to evaluate an influence of rush VIT on MC markers in treated children. materials and methods. Sensitized group: 25 children with SR to Hymenoptera sting. Control group: 19 healthy children. Active treatment: 5-day-rush-VIT. BST was evaluated by ImmunoCAP, PGD[sub]2[/sub] metabolites in blood and urine by GC-NICI-MS. results. The baseline blood levels of MC markers were significantly higher, while urinary concentration of 9α,11β-PGF2 was significantly lower in the whole group of venom-sensitized children compared to controls. Severity of SR showed negative correlation with urinary PGD[sub]2[/sub] metabolites, while positive with plasma 9α,11β-PGF2 and BST concentration The highest sensitivity was obtained for plasma 9α,11β-PGF2 whereas the highest specificity for urinary PGD-M. conclusions. In children with IgE-mediated SR to Hymenoptera stings, elevation of baseline values of PGD2 metabolites in blood is accompanied by decreased excretion of its urinary metabolites. Assessment of stable PGD[sub]2 [/sub] metabolites might serve as an independent MC marker to identify allergic children. There is an association between urinary PGD[sub]2[/sub] metabolites and severity of the SR to Hymenoptera stings.

  12. Meteorus arizonensis Muesebeck, 1923 (Hymenoptera: Braconidae): nuevo registro para México

    OpenAIRE

    Gutiérrez-Ramírez, A.; Robles-Bermúdez, A.; Cambero-Campos, J.; Coronado-Blanco, J. M.

    2015-01-01

    Braconidae is one of the more diverse families of Hymenoptera with almost 20,000 species worldwide. Meteorus has 316 species and is almost cosmopolitan. Previously, seven species of this genus have been recorded from Mexico. In this note, Meteorus arizo-nensis Muesebeck is recorded for the first time for the country, obtained from the fall armyworm, Spodoptera frugiperda (J.E. Smith), with material from Santa Maria del Oro, locality in the State of Nayarit.

  13. Parasitoidism of Chalcidid wasps (Hymenoptera, Chalcididae on Philornis sp. (Diptera, Muscidae

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    M. S. Couri

    Full Text Available Philornis Meinert larvae are known as parasites of birds, with coprophagous, semi-hematophagous or hematophagous habits. Biological data of the larvae of the fifty described species are still scarcely known. Here we describe some aspects of the parasitism of a species of Philornis on Thalurania glaucopis Gmelin (Trochilidae and record two species of Chalcididae (Hymenoptera parasitoids, Conura annulifera (Walker, 1864 and Brachymeria podagrica (Fabricius, 1787, reared from Philornis puparia.

  14. The species of the genus Hypodynerus de Saussure (Hymenoptera, Vespidae, Eumeninae) occurring in Brazil.

    Science.gov (United States)

    Garcete-Barrett, Bolívar R; Hermes, Marcel Gustavo

    2013-01-01

    An identification table and descriptions are given to recognize the two species of Hypodynerus (Hymenoptera: Vespidae: Eumeninae) recorded from Brazil: Hypodynerus arechavaletae (Brèthes) and Hypodynerus duckei (Bertoni) comb. n. The lectotype is designated and the male is described for Hypodynerus duckei, its presence being recorded from Brazil for the first time.

  15. Evaluation of the quality of life in subjects with a history of severe anaphylactic reaction to the Hymenoptera venom.

    Science.gov (United States)

    Nowak, Natalia; Bazan-Socha, Stanisława; Pulka, Grażyna; Pełka, Karolina; Latra, Paulina

    2015-01-01

    Sensitization to the Hymenoptera venom is one of the main causes of anaphylaxis in Poland. Venom immunotherapy is the only effective treatment in such cases. Comprehensive patient care includes also education. The aim of our study was to assess the state of knowledge and to evaluate the quality of life and the anxiety level in patients allergic to the Hymenoptera venom after anaphylactic reaction. The survey was carried out in the period of the insects flight in 61 adult subjects (35 wasp and 26 bee allergic), using a validated Vespid Allergy Quality of Life Questionnaire (VQLQ), Hospital Anxiety and Depression Scale, and subjective assessment of anxiety level. The majority of respondents received venom immunotherapy. Sensitized to the wasp venom had significantly impaired quality of life (VQLQ score) as compared to the bee venom allergic (p = 0.014). The intensity of anxiety decreased with the duration of immunotherapy (p = 0.01). The majority of subjects knew how to recognize and treat anaphylaxis, but only 8% employed an identification card and about 50% implemented rules of the pre-exposition prophylaxis. History of a severe anaphylaxis to the Hymenoptera venom affected the quality of life. Venom immunotherapy reduced anxiety. We hope that presented surveys and their results might be useful in qualifying for immunotherapy in clinically uncertain cases.

  16. A new species of Chelonus (Areselonus) (Hymenoptera, Braconidae) from India reared from Acrocercops lysibathra (Meyrick) on Cordia latifolia Roxb.

    OpenAIRE

    Ahmad, Zubair; Ghramh, Hamed A.

    2018-01-01

    Chelonus (Areselonus) spinigaster sp. n., (Hymenoptera: Braconidae: Cheloninae) is described from India. The new species was reared from the moth species Acrocercops lysibathra (Meyr.) on Cordia latifolia Roxb.

  17. A new species of Chelonus (Areselonus) (Hymenoptera, Braconidae) from India reared from Acrocercops lysibathra (Meyrick) on Cordia latifolia Roxb.

    Science.gov (United States)

    Ahmad, Zubair; Ghramh, Hamed A

    2018-01-01

    Chelonus (Areselonus) spinigaster sp. n. , (Hymenoptera: Braconidae: Cheloninae) is described from India. The new species was reared from the moth species Acrocercops lysibathra (Meyr.) on Cordia latifolia Roxb.

  18. First report of Eurytoma plotnikovi Nik. (Hymenoptera, Eurytomidae, a seed parasite of pistachio, in Sicily (Italy

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    Santi Longo

    2011-09-01

    Full Text Available The pistachio seed wasp, Eurytoma plotnikovi Nik.(Hymenoptera, E urytomidae, is a new pest recently arrived in pistachio orchards in central-western Sicily (Italy. Information on the damaging effects of this seed wasp in the affected areas is provided.

  19. Field behavior of parasitic Coelioxys chichimeca (Hymenoptera: Megachilidae) toward the host bee Centris bicornuta (Hymenoptera: Apidae)

    OpenAIRE

    Vinson , S.; Frankie , Gordon; Rao , Asha

    2011-01-01

    International audience; Coelioxys chichimeca is a cleptoparasite and an important cause of mortality for several woodhole nesting pollinator bees in Costa Rica. We studied the behavior of this parasitic bee towards one of the more common host bee, Centris (Heterocentris) bicornuta. The female parasitic C. chichimeca were attracted to host bee, C. bicornuta nest cells that contained pollen, but only exhibited interest in entering and ovipositing when these host cells contained nectar. This int...

  20. Seasonality of Pelecinus polyturator (Drury (Hymenoptera, Pelecinidae in the Atlantic Rainforest of São Paulo State, Brazil

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    Rogéria I. R. Lara

    2014-03-01

    Full Text Available Seasonality of Pelecinus polyturator (Drury (Hymenoptera, Pelecinidae in the Atlantic Rainforest of São Paulo State, Brazil. A survey of the parasitoid wasp Pelecinus polyturator (Drury, 1773 (Hymenoptera, Pelecinidae was carried out with five Malaise traps/area in five areas in the Atlantic Rainforest of São Paulo State, Brazil, between November 2009 and October 2010. The sampling effort in each locality amounted to 1,825 trap-days. Data were obtained from a total of 317 exemplars of P. polyturator, corresponding to 108 females and 209 males. The average sex ratio of the studied population was 0.52. The highest occurrence of P. polyturator was observed between November and March with frequency peak in January; about 95% of the specimens studied were captured at altitudes close to 1,000 m above sea level.

  1. Body size limits dim-light foraging activity in stingless bees (Apidae: Meliponini).

    Science.gov (United States)

    Streinzer, Martin; Huber, Werner; Spaethe, Johannes

    2016-10-01

    Stingless bees constitute a species-rich tribe of tropical and subtropical eusocial Apidae that act as important pollinators for flowering plants. Many foraging tasks rely on vision, e.g. spatial orientation and detection of food sources and nest entrances. Meliponini workers are usually small, which sets limits on eye morphology and thus quality of vision. Limitations are expected both on acuity, and thus on the ability to detect objects from a distance, as well as on sensitivity, and thus on the foraging time window at dusk and dawn. In this study, we determined light intensity thresholds for flight under dim light conditions in eight stingless bee species in relation to body size in a Neotropical lowland rainforest. Species varied in body size (0.8-1.7 mm thorax-width), and we found a strong negative correlation with light intensity thresholds (0.1-79 lx). Further, we measured eye size, ocelli diameter, ommatidia number, and facet diameter. All parameters significantly correlated with body size. A disproportionately low light intensity threshold in the minute Trigonisca pipioli, together with a large eye parameter P eye suggests specific adaptations to circumvent the optical constraints imposed by the small body size. We discuss the implications of body size in bees on foraging behavior.

  2. Genes underlying reproductive division of labor in termites, with comparisons to social Hymenoptera

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    Judith eKorb

    2016-04-01

    Full Text Available All social insects are characterized by a reproductive division of labor. Within a colony only a few individuals reproduce (queens and in termites, also a king while the large majority (workers and soldiers forgo reproduction, at least temporarily. The evolution of such reproductive altruism can ultimately be explained by inclusive fitness theory. Here, I will review the proximate genetic mechanisms underlying this altruism in termites. As social cockroaches they evolved eusociality independently from the social Hymenoptera, which makes them interesting test cases to look for common underlying mechanisms of eusociality and lineage specific idiosyncrasies. First, I will provide a summary of the genes and their function that have been identified to underlie reproductive division of labor - so called 'queen genes,' - in the drywood termite Cryptotermes secundus, an emerging model to study termite social evolution. Second, I outline how widespread these queen genes are across the termite phylogeny, using also evidence from recent genome analyses. I will provide hypotheses about the evolutionary origin of these queen genes, aiming to link proximate mechanisms with ultimate functions. Finally, I will draw comparisons to social Hymenoptera to indicate potential common underpinnings that warrant further testing.

  3. The Hymenopterous Poison Apparatus. X. Morphological and Behavioral Changes in Atta texana (Hymenoptera: Formicidae)

    Science.gov (United States)

    Henry R. Hermann; John C. Moser; Allen N. Hunt

    1970-01-01

    Atta texana (Buckley) and other members of this genus no longer utilize the 8th and 9th gonapophyses as part of their defensive system. Although the sclerites that comprise the stinging apparatus in most aculeate Hymenoptera are present in the species, they seem to function only in the deposition of trail pheromones. A mechanical and chemical defense...

  4. The species of the genus Hypodynerus de Saussure (Hymenoptera, Vespidae, Eumeninae occurring in Brazil

    Directory of Open Access Journals (Sweden)

    Bolívar Garcete-Barrett

    2013-04-01

    Full Text Available An identification table and descriptions are given to recognize the two species of Hypodynerus (Hymenoptera: Vespidae: Eumeninae recorded from Brazil: H. arechavaletae (Brèthes and H. duckei (Bertoni comb. n. The lectotype is designated and the male is described for Hypodynerus duckei, its presence being recorded from Brazil for the first time.

  5. Reproductive and developmental biology of the emerald ash borer parasitoid Spathius galinae (Hymenoptera: Braconidae) as affected by temperature

    Science.gov (United States)

    Emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) is an invasive pest of serious concern in North America. To complement ongoing biological control efforts, Spathius galinae Belokobylskij and Strazenac (Hymenoptera: Braconidae), a recently-described specialist parasitoid of ...

  6. Primeiro registro de ocorrência do parasitóide Brasema sp. (hymenoptera: eupelmidae em ovos de Leptoglossus zonatus (Dallas, 1852 (hemiptera: coreidae no Brasil First record of occurrence of the parasitoid Brasema sp. (hymenoptera: eupelmidae in eggs of Leptoglossus zonatus (Dallas, 1852 (hemiptera: coreidae in Brazil

    Directory of Open Access Journals (Sweden)

    Carlos Henrique Marchiori

    2002-12-01

    Full Text Available Este trabalho registra a primeira ocorrência do parasitóide Brasema sp. (Hymenoptera: Eupelmidae parasitando ovos de Leptoglossus zonatus (Hemiptera: Coreidae em cultivar de milho em Itumbiara, GO. A porcentagem de parasitismo foi de 4,8%. Brasema sp. constitue-se em ectoparasitóide de larvas de coleópteros e de outros hospedeiros no interior de tecidos de plantas.This work reports, for the first time, of parasitoid Brasema sp. (Hymenoptera: Eupelmidae parasitizinf eggs of Leptoglossus zonatus (Hemiptera: Coreidae in maize cultivate in Itumbiara, GO, Brazil. The percentage of parasitizing was 4,8%. Brasema sp. constitutes ectoparasitoid of beetle larvae and other hosts concealed in plant tissue.

  7. Sex determination in the haplodiploid wasp Nasonia vitripennis (Hymenoptera Chalcidoidea) : A critical consideration of models and evidence

    NARCIS (Netherlands)

    Beukeboom, Leo W.; Kamping, Albert; van de Zande, Louis

    Sex determining mechanisms are highly diverse. Like all Hymenoptera, the parasitic wasp Nasonia vitripennis reproduces by haplodiploidy: males are haploid and females are diploid. Sex in Nasonia is not determined by complementary alleles at sex loci. Evidence for several alternative models is

  8. Two new species of Oobius (Hymenoptera: Encyrtidae) and their phylogenetic relationship with other congeners from northeastern Asia

    Science.gov (United States)

    Yan-Xia Yao; Jian J. Duan; Jason L. Mottern; Xiao-Yi Wang; Zhong-Qi Yang; Leah S. Bauer; Michael W. Gates

    2018-01-01

    Two new species of egg parasitoids, Oobius saimaensis Yao and Mottern new species and Oobius fleischeri Yao and Duan new species (Hymenoptera: Encyrtidae), are described from eggs of Agrilus fleischeri Obenberger, 1925 (Coleoptera: Buprestidae). Agrilus fleischeri is a phloemfeeding woodborer of poplar (...

  9. Aspectos da biologia de nidificação de Euplusia mussitans (Fabricius (Hymenoptera, Apidae, Euglossini Aspects of nesting biology of Euplusia mussitans Fabricius (Hymenoptera, Apidae, Euglossini

    Directory of Open Access Journals (Sweden)

    Blandina Felipe Viana

    2001-12-01

    Full Text Available The architecture and biology of Euplusia mussitans (Fabricius, 1787 nests were investigated, using trap nests made by wood, which were randomly distributed in a fragment of a coastal sand dunes, in Salvador, Bahia. Brazil (12º56'S and 38º21'W. 10 nests were found in cavities with 1.5 cm in diameter. From them emerged 10 females and 16 males (Sex ratio = 0,38. The nest cells were made of small pieces of bark cemented together with resin. The number of cells in the nests varied from 2 to 4, with females cells at the inner end and males cells towards the entrance. No significant difference was observed (t= 0.7274, P > 0.05 between the medium size for males (6.3 mm ± 0.16 and for females (6.4 mm ± 0.29. The development time of females was longer than males.

  10. Nidificação e forrageamento de Centris (Ptilotopus maranhensis Ducke (Hymenoptera, Apidae, Centridini Nesting biology and foraging of Centris (Ptilotopus maranhensis Ducke (Hymenoptera, Apidae, Centridini

    Directory of Open Access Journals (Sweden)

    Marina Ramos

    2007-12-01

    Full Text Available As abelhas pertencentes à tribo Centridini possuem distribuição neotropical e são consideradas os principais polinizadores de diversas espécies vegetais em diferentes biomas. Apesar disso, dados sobre a biologia da maioria das espécies ainda são escassos. Este trabalho relata observações sobre a biologia de Centris (Ptilotopus maranhensis Ducke, 1910, uma espécie que ocorre no Nordeste do Brasil. O estudo foi conduzido em uma área de cerrado no Estado do Maranhão durante o período de atividade dos adultos. Fêmeas foram observadas nidificando em termiteiros epígeos e em visita às flores de Byrsonima umbellata Mart. (Malpighiaceae para coleta de óleo. O comportamento foi registrado diretamente e através de filmagens. Os ninhos foram escavados, raspando-se o substrato até as células serem encontradas. Um ninho completo foi aberto para exame das estruturas internas e análise do conteúdo das células. A estrutura geral apresentava um túnel principal com células descendentes dispostas linearmente. Foram encontradas células (n = 5 com larvas em diferentes estágios de desenvolvimento e uma massa de pólen de consistência firme e pastosa formada por uma grande quantidade de grãos de Byrsonima.The bees belonging to the tribe Centridini possess distribution neotropical and are considered the main pollinators of different plant species in different biomas. Despite of this the biology data of the majority of species remain scarce. This work is about observations of biology of Centris (Ptilotopus maranhensis Ducke, 1910, specie that occurs in the Northeast of Brazil. The study was made in a cerrado area in the State of Maranhão during a period of adult activity. Females were seen nesting at epigeous termite nests and visiting Byrsonima umbellata Mart. (Malpighiaceae flowers for oil collection. The behavior was directly registered and shooted. The nests were excavated being scraped the substratum until the cells were found. A complete nest was opened for examination of the internal structures and analysis of cells content. The general structure presented a main tunnel with cells descending linearly. Cells (n = 5 with larvae in different periods of development were found. A firmly pollen paste was seen where a huge Byrsonima grains was verified.

  11. Acceptance threshold hypothesis is supported by chemical similarity of cuticular hydrocarbons in a stingless bee, Melipona asilvai.

    Science.gov (United States)

    Nascimento, D L; Nascimento, F S

    2012-11-01

    The ability to discriminate nestmates from non-nestmates in insect societies is essential to protect colonies from conspecific invaders. The acceptance threshold hypothesis predicts that organisms whose recognition systems classify recipients without errors should optimize the balance between acceptance and rejection. In this process, cuticular hydrocarbons play an important role as cues of recognition in social insects. The aims of this study were to determine whether guards exhibit a restrictive level of rejection towards chemically distinct individuals, becoming more permissive during the encounters with either nestmate or non-nestmate individuals bearing chemically similar profiles. The study demonstrates that Melipona asilvai (Hymenoptera: Apidae: Meliponini) guards exhibit a flexible system of nestmate recognition according to the degree of chemical similarity between the incoming forager and its own cuticular hydrocarbons profile. Guards became less restrictive in their acceptance rates when they encounter non-nestmates with highly similar chemical profiles, which they probably mistake for nestmates, hence broadening their acceptance level.

  12. The activity of carbohydrate-degrading enzymes in the development of brood and newly emerged workers and drones of the Carniolan honeybee, Apis mellifera carnica.

    Science.gov (United States)

    Żółtowska, Krystyna; Lipiński, Zbigniew; Łopieńska-Biernat, Elżbieta; Farjan, Marek; Dmitryjuk, Małgorzata

    2012-01-01

    The activity of glycogen Phosphorylase and carbohydrate hydrolyzing enzymes α-amylase, glucoamylase, trehalase, and sucrase was studied in the development of the Carniolan honey bee, Apis mellifera carnica Pollman (Hymenoptera: Apidae), from newly hatched larva to freshly emerged imago of worker and drone. Phosphorolytic degradation of glycogen was significantly stronger than hydrolytic degradation in all developmental stages. Developmental profiles of hydrolase activity were similar in both sexes of brood; high activity was found in unsealed larvae, the lowest in prepupae followed by an increase in enzymatic activity. Especially intensive increases in activity occurred in the last stage of pupae and newly emerged imago. Besides α-amylase, the activities of other enzymes were higher in drone than in worker broods. Among drones, activity of glucoamylase was particularly high, ranging from around three times higher in the youngest larvae to 13 times higher in the oldest pupae. This confirms earlier suggestions about higher rates of metabolism in drone broods than in worker broods.

  13. Effects of venom immunotherapy on serum level of CCL5/RANTES in patients with Hymenoptera venom allergy.

    Science.gov (United States)

    Gawlik, Radoslaw; Glück, Joanna; Jawor, Barbara; Rogala, Barbara

    2015-01-01

    Hymenoptera venoms are known to cause life-threatening IgE-mediated anaphylactic reactions in allergic individuals. Venom immunotherapy is a recommended treatment of insect allergy with still the mechanism not being completely understood. We decided to assess the serum CCL5/RANTES level in patients who experienced severe anaphylactic reaction to Hymenoptera venom and to find out changes in the course of immunotherapy. Twenty patients (9 men, 11 women, mean age: 31.91 ± 7.63 years) with history of anaphylactic reaction after insect sting were included into the study. Diagnosis was made according to sIgE and skin tests. All of them were enrolled into rush venom immunotherapy with bee or wasp venom extracts (Pharmalgen, ALK-Abello, Horsholm, Denmark). Serum levels of CCL5/RANTES were measured using a commercially available ELISA kit (R&D Systems, Minneapolis, MN). CCL5/RANTES serum concentration are higher in insect venom allergic patients than in healthy controls (887.5 ± 322.77 versus 387.27 ± 85.11 pg/ml). Serum concentration of CCL5/RANTES in insect venom allergic patient was significantly reduced in the course of allergen immunotherapy already after 6 days of vaccination (887.5 ± 322.77 versus 567.32 ± 92.16 pg/ml). CCL5/RANTES serum doesn't correlate with specific IgE. Chemokine CCL5/RANTES participates in allergic inflammation induced by Hymenoptera venom allergens. Specific immunotherapy reduces chemokine CCL5/RANTES serum level already after initial days of venom immunotherapy.

  14. Primer registro del género Mellinus (Hymenoptera: Sphecidae en Colombia

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    Fernández C. Fernando

    1999-06-01

    Full Text Available Uno de los taxones menos conocidos de Sphecidae (Hymenoptera: Apoidea es Mellinus Fabricius, género que en el pasado llegó a tener categoría de subfamilia (Bohart & Menke 1976, pero que ahora comprende una tribu, Mellinini, dentro de la subfamilia Nyssoninae (Menke & Fernández 1996. Al parecer,  las hembras de este género capturan moscas del estiércol cerca a excrementos de mamíferos en el campo, y hacen sus nidos en el suelo (Evans 1989.

  15. Ciclo de desarrollo de Trigona (Tetragonisca angustula, Latreille 1811 (Hymenoptera, Trigonini

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    María Giomar Nates Parra

    1989-01-01

    Full Text Available Se estudió el ciclo de desarrollo de una abeja sin aguijón: Trigona (Tetragonisca angustula. Latreille 1811 (Hymenoptera. Trigonini. Los resultados fueron los siguientes: El periodo comprendido entre la postura del huevo y la emergencia del imago, es, en las obreras, de 36.5 días en promedio. La duración del ciclo se hace mayor a medida que las celdas se alejan del centro del panal. Se determinaron 3 instares larvales y 5 fases pupales para obreras.

  16. Role of emerald ash borer (Coleoptera: Buprestidae) larval vibrations in host-quality assessments by Tetrastichus planipennisi (Hymenoptera: Eulophidae)

    Science.gov (United States)

    Michael D. Ulyshen; Richard W. Mankin; Yigen Chen; Jian J. Duan; Therese M. Poland; Leah S. Bauer

    2011-01-01

    The biological control agent Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) is a gregarious larval endoparasitoid of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), an invasive cambium-feeding species responsible for recent, widespread mortality of ash (Fraxinus spp.) in...

  17. Pollination Requirements of Almond (Prunus dulcis): Combining Laboratory and Field Experiments.

    Science.gov (United States)

    Henselek, Yuki; Eilers, Elisabeth J; Kremen, Claire; Hendrix, Stephen D; Klein, Alexandra-Maria

    2018-03-08

    Almond (Prunus dulcis (Mill.) D. A. Webb; Rosales: Rosaceae) is a cash crop with an estimated global value of over seven billion U.S. dollars annually and commercial varieties are highly dependent on insect pollination. Therefore, the understanding of basic pollination requirements of the main varieties including pollination efficiency of honey bees (Apis mellifera, Linnaeus, Hymenoptera: Apidae) and wild pollinators is essential for almond production. We first conducted two lab experiments to examine the threshold number of pollen grains needed for successful pollination and to determine if varietal identity or diversity promotes fruit set and weight. Further, we examined stigma and ovules of flowers visited by Apis and non-Apis pollinators in the field to study the proportion of almond to non-almond pollen grains deposited, visitation time per flower visit, and tube set. Results indicate that the threshold for successful fertilization is around 60 pollen grains, but pollen can be from any compatible variety as neither pollen varietal identity nor diversity enhanced fruit set or weight. Andrena cerasifolii Cockerell (Hymenoptera: Andrenidae) was a more effective pollinator on a per single visit basis than Apis and syrphid flies. Nevertheless, Apis was more efficient than A. cerasifolii and syrphid flies as they spent less time on a flower during a single visit. Hence, planting with two compatible varieties and managing for both Apis and non-Apis pollinators is likely to be an optimal strategy for farmers to secure high and stable pollination success.

  18. Invasive ant Tapinoma melanocephalum (Hymenoptera: Formicidae): A rare quest or increasingly common indoor pest in Europe?

    Czech Academy of Sciences Publication Activity Database

    Klimeš, Petr; Okrouhlík, Jan

    2015-01-01

    Roč. 112, č. 4 (2015), s. 705-712 ISSN 1210-5759 R&D Projects: GA ČR GB14-36098G Institutional support: RVO:60077344 Keywords : Hymenoptera * Formicidae * Tapinoma Subject RIV: EH - Ecology, Behaviour Impact factor: 0.975, year: 2014 http://www.eje.cz/pdfs/eje/2015/04/16.pdf

  19. The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae, a parasitoid wasp of Drosophila

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    Daniel S. Oliveira

    Full Text Available Abstract The partial mitochondrial genome sequence of Leptopilina boulardi (Hymenoptera: Figitidae was characterized. Illumina sequencing was used yielding 35,999,679 reads, from which 102,482 were utilized in the assembly. The length of the sequenced region of this partial mitochondrial genome is 15,417 bp, consisting of 13 protein-coding, two rRNA, and 21tRNA genes (the trnaM failed to be sequenced and a partial A+T-rich region. All protein-coding genes start with ATN codons. Eleven protein-coding genes presented TAA stop codons, whereas ND6 and COII that presented TA, and T nucleotides, respectively. The gene pattern revealed extensive rearrangements compared to the typical pattern generally observed in insects. These rearrangements involve two protein-coding and two ribosomal genes, along with the 16 tRNA genes. This gene order is different from the pattern described for Ibalia leucospoides (Ibaliidae, Cynipoidea, suggesting that this particular gene order can be variable among Cynipoidea superfamily members. A maximum likelihood phylogenetic analysis of the main groups of Apocrita was performed using amino acid sequence of 13 protein-coding genes, showing monophyly for the Cynipoidea superfamily within the Hymenoptera phylogeny.

  20. Epidemiology of allergic reactions to hymenoptera stings in Irish school children.

    LENUS (Irish Health Repository)

    Jennings, Aisling

    2010-12-01

    The aim of this was to study generate the first epidemiological data regarding the prevalence of hymenoptera allergy among school children in Ireland. Questionnaires, including six sting-specific questions (1), were distributed to the parents of primary school children aged 6-8 and 11-13, divided equally between rural and urban backgrounds. From 110 schools, 4112 questionnaires were returned. A total of 1544 (37.5%) children had been stung in their lifetime. Among the total, 5.8% of children stung experienced a large local reaction, 3.4% had a mild (cutaneous) systemic reaction (MSR) and 0.8% experienced a moderate\\/severe systemic reaction (SSR); these figures respectively represent 2.2%, 1.3% and 0.2% of the total study group. On logistic regression analysis, older children and rural children were at a higher risk of being stung (OR 1.7; 95% CI 1.4-2.; OR 1.6; 95% CI 1.4-1.8 respectively). Rural dwellers and asthma sufferers were more likely to experience an SSR (OR 4.3; 95% CI 1.4-13.5 and OR 2.8; 95% CI 1.8-4.3, respectively). Hymenoptera stings are more common in rural than urban dwelling Irish children. Asthma imparted a greater risk of SSR in this study population. Severe reactions are unusual overall, occurring in <1% of those stung, a lower prevalence than in Israeli teenagers but in keeping with other European reports relating to young children.

  1. Abelhas (Hymenoptera: Apoidea visitantes das flores do feijão guandu no Recôncavo Baiano, Brasil Bees (Hymenoptera: Apoidea on pigeonpea flowers in 'Recôncavo Baiano' region, Brazil

    Directory of Open Access Journals (Sweden)

    Ruberval Leone Azevedo

    2007-10-01

    Full Text Available O feijão guandu Cajanus cajan L. é uma cultura comum, encontrada com freqüência em todo o Brasil, principalmente em pequenas propriedades rurais. Este trabalho teve por objetivo obter informações sobre a diversidade de abelhas visitantes das flores do feijão guandu, gerando informações sobre a estrutura da comunidade de abelhas e a importância desta leguminosa como fonte de recursos tróficos. As coletas foram feitas semanalmente, entre maio e outubro de 2005, na área experimental de Entomologia do Centro de Ciências Agrárias da UFBA, localizada no município de Cruz das Almas, Bahia, no intervalo entre as 6:00 e as 18:00 horas. Um total de 4.676 indivíduos foi coletado visitando as flores do feijão guandu. A família Apidae foi representada por 99,3% dos indivíduos e 76,2% das espécies amostradas. Os gêneros Xylocopa e Trigona foram os que apresentaram maior riqueza, com quatro e três espécies, respectivamente. Trigona spinipes foi a espécie mais abundante, com freqüência relativa igual a 83,1%, seguida de Nannotrigona testaceicornis (5,1%.Pigeonpea, Cajanus cajan L. is a common crop, frequently found throughout Brazil, mainly in small rural properties. This research was aimed at geting information on the diversity of visiting bees to flowers of pigeonpea, generating information on the structure of the bee community and the importance of this Leguminosae as source of food resources. Collections had been made weekly, between May and October 2005 in the Experimental area of the Center of Agrarian Sciences and Environmental of the UFBA, located in the Cruz das Almas, Bahia, in the interval between 6:00 AM and 06:00 PM. A total of 4.676 individuals was collected visiting the pigeonpea flowers. The Apidae family was represented by 99.25% of individuals and 76.19% of total species. The genera Xylocopa and Trigona were the ones that showed greater richness, with four and three species, respectively. Trigona spinipes was the most

  2. Hyperparasitoid wasps (Hymenoptera: Trigonalidae) reared from dry forest and rain forest caterpillars of Area de Conservacion, Guanacaste, Costa Rica

    Science.gov (United States)

    Five species of Trigonalidae, hyperparasites of Ichneumonidae (Hymenoptera) and Tachinidae (Diptera) that parasitize caterpillars (Lepidoptera), have been reared during the ongoing caterpillar inventory of Area de Conservación Guanacaste (ACG), Guanacaste Province, northwestern Costa Rica: Lycogaste...

  3. A new species of Megischus Brullé (Hymenoptera, Stephanidae from China, with a key to the Chinese species

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    Hong Chun-dan

    2010-11-01

    Full Text Available A new species of Megischus Brullé from China, M. aplicatus sp. n., is described and illustrated. A key to the Chinese species of Megischus is added. The holotype is deposited in the Parasitic Hymenoptera Collection of Zhejiang University, Hangzhou.

  4. A new species of solitary Meteorus (Hymenoptera: Braconidae) reared from caterpillars of toxic butterflies (Lepidoptera: Nymphalidae) in Ecuador.

    Science.gov (United States)

    Shaw, Scott R; Jones, Guinevere Z

    2009-01-01

    A new species of parasitoid wasp, Meteorus rugonasus Shaw and Jones (Hymenoptera: Braconidae), is described from the Yanayacu Biological Station, Napo Province, Ecuador. The new species is diagnosed and compared to other species in the genus. It was reared from larvae of Pteronymia zerlina (Hewitson, 1855) (Lepidoptera: Nymphalidae, Ithomiinae) found feeding on leaves of Solanum (Solanaceae). The parasitoid is solitary. This is the first record of a Meteorus species attacking ithomiine Nymphalidae. A new species of parasitoid wasp, Meteorus rugonasus Shaw and Jones (Hymenoptera: Braconidae), is described from the Yanayacu Biological Station, Napo Province, Ecuador. The new species is diagnosed and compared to other species in the genus. It was reared from larvae of Pteronymia zerlina (Hewitson, 1855) (Lepidoptera: Nymphalidae, Ithomiinae) found feeding on leaves of Solanum (Solanaceae). The parasitoid is solitary. This is the first record of a Meteorus species attacking ithomiine Nymphalidae.

  5. Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome.

    Science.gov (United States)

    de Sousa, Cristina Soares; Serrão, José Eduardo; Bonetti, Ana Maria; Amaral, Isabel Marques Rodrigues; Kerr, Warwick Estevam; Maranhão, Andréa Queiroz; Ueira-Vieira, Carlos

    2013-07-01

    The insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in stingless bees. In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The M. scutellaris fat body ESTs encoded proteins with roles in numerous physiological processes, including anti-oxidation, phosphorylation, metabolism, detoxification, transmembrane transport, intracellular transport, cell proliferation, protein hydrolysis and protein synthesis. This is the first report to describe a transcriptomic analysis of specific organs of M. scutellaris. Our findings provide new insights into the physiological role of the fat body in stingless bees.

  6. Morphogenetic Alterations in Melipona quadrifasciata anthidioides (Hymenoptera: Apidae) Associated with Pesticides.

    Science.gov (United States)

    Prado-Silva, Arlete; Nunes, Lorena Andrade; Dos Santos, Jádilla Mendes; Affonso, Paulo Roberto Antunes de Mello; Waldschmidt, Ana Maria

    2018-05-01

    Bees are major pollinators of both native flora and cultured crops. Nonetheless, despite their key functional role in ecosystems and agriculture, bee populations have been affected worldwide by deforestation and contamination by insecticides. Conversely, little is known about the effects of pesticides on morphogenetic development of neotropical stingless bees. We compared the fluctuating asymmetry (FA) in newly emerged bees and foragers of Melipona quadrifasciata anthidioides exposed to pesticides (experimental greenhouse and cultivated field). In addition, visitation behavior of foragers was inferred from pollen analyses and direct observation. A significant increase of FA (P < 0.001) was detected in bees from the greenhouse. Even though pesticides might affect their development, foragers seem to avoid contaminated plants whenever possible, as confirmed by pollen and visitation analyses. Consequently, the conservation of natural forests in agricultural landscapes is essential to ensure the health of colonies in stingless bees.

  7. Predatory behavior in a necrophagous bee Trigona hypogea (Hymenoptera; Apidae, Meliponini)

    Science.gov (United States)

    Mateus, Sidnei; Noll, Fernando B.

    Although most bees feed on nectar and pollen, several exceptions have been reported. The strangest of all is the habit found in some neotropical stingless bees, which have completely replaced pollen-eating by eating animal protein from corpses. For more than 20 years, it was believed that carrion was the only protein source for these bees. We report that these bees feed not only off dead animals, but on the living brood of social wasps and possibly other similar sources. Using well developed prey location and foraging behaviors, necrophagous bees discover recently abandoned wasps' nests and, within a few hours, prey upon all immatures found there.

  8. A review of the albidohirta group of Ptilothrix (Hymenoptera: Apidae: Emphorini

    Directory of Open Access Journals (Sweden)

    Arturo ROIG ALSINA

    2007-01-01

    Full Text Available El grupo albidohirta de Ptilothrix Smith incluye tres especies: Ptilothrix albidohirta Brèthes, P. concolor sp. nov. y P. nemoralis sp. nov. Este grupo se conoce del oeste y norte de la Argentina y del centro de Bolivia. Una de sus especies, P. albidohirta, llega hasta los 3.600 m s.n.m. en la cordillera de los Andes, en tanto que las otras dos especies habitan en el llano. Se caracteriza el grupo y se describen e ilustran las especies.

  9. TUMBUHAN YANG DIKUNJUNGI LEBAH PEKERJA Apis (Hymenoptera: Apidae DI SUMATERA BARAT

    Directory of Open Access Journals (Sweden)

    Jasmi Jasmi

    2016-09-01

    Full Text Available Research about plant visited by Apis forager was conducted at altitude < 500 m and 600-1400 m of West Sumatra from December 2009-July 2010. The plant visited by forager was collected at radius 500 m from nests in fruits cropping, coffea and cinnamom plantation and forest edge. Honeybee from genus Apisvisit on plant consisted of four species, those are A. andreniformis, A. cerana, A. dorsata and A. koschevnikovi. Plant visited by forager Apis consisted of 61 species belong to 26 families. The number of 36 plant species found at lowland and highland, 20 species only at lowland and five species at highland. Most of plant species visited by Apis forager are Asteraceae and Leguminoceae. About 29 plant species visited by Apis were agriculture crop and 32 species of non agriculture. Apis andreniformis visited 29 plant species, A. cerana 56 species, A. dorsata 57 species and A. koschevnikovi one plant species. Cucumis sativus was visited by four bee species, Galiansoga farviflora was visited only by A. cerana. Five plant species (Rorippa indica, Cinnamomum burmanii, Nasturrium indicum, Rorippa indica and Eryngium foetidum were visited by only A. dorsata. Other three plants species (Mangifera indica, Cytrus aurantifoliaand Oryza sativa were visited by A. cerana and A. andreniformis, 31 plant species were visited by A. cerana and A. dorsata, 21 species were visited by A. andreniformis, A. cerana and A. dorsata. Keywords: lebah madu, Apis forager, food source plant, visit

  10. The apid cuckoo bees of the Cape Verde Islands (Hymenoptera, Apidae).

    Science.gov (United States)

    Straka, Jakub; Engel, Michael S

    2012-01-01

    The apid cuckoo bees of the Cape Verde Islands (Republic of Cape Verde) are reviewed and five species recognized, representing two genera. The ammobatine genus Chiasmognathus Engel (Nomadinae: Ammobatini), a specialized lineage of cleptoparasites of nomioidine bees is recorded for the first time. Chiasmognathus batelkaisp. n. is distinguished from mainland African and Asian species. The genus Thyreus Panzer (Apinae: Melectini) is represented by four species - Thyreus denoliisp. n., Thyreus batelkaisp. n., Thyreus schwarzisp. n., and Thyreus aistleitnerisp. n. Previous records of Thyreus scutellaris (Fabricius) from the islands were based on misidentifications.

  11. Application of a modified selection index for honey bees (Hymenoptera: Apidae).

    Science.gov (United States)

    van Engelsdorp, D; Otis, G W

    2000-12-01

    Nine different genetic families of honey bees (Apis mellifera L.) were compared using summed z-scores (phenotypic values) and a modified selection index (Imod). Imod values incorporated both the phenotypic scores of the different traits and the economic weightings of these traits, as determined by a survey of commercial Ontario beekeepers. Largely because of the high weight all beekeepers place on honey production, a distinct difference between line rankings based on phenotypic scores and Imod scores was apparent, thereby emphasizing the need to properly weight the traits being evaluated to select bee stocks most valuable for beekeepers. Furthermore, when beekeepers who made >10% of their income from queen and nucleus colony sales assigned relative values to the traits used in the Imod calculations, the results differed from those based on weightings assigned by honey producers. Our results underscore the difficulties the North American beekeeping industry must overcome to devise effective methods of evaluating colonies for breeding purposes.

  12. Stingless Bees (Hymenoptera: Apidae: Meliponini in Oriental Mountains Cementeries from Colombia

    Directory of Open Access Journals (Sweden)

    Guiomar Nates-Parra

    2006-01-01

    Full Text Available In 11 cemeteries of Cundinamarca and Meta (Colombia departments we found 203 nests of stingless bees pertaining to 15 species. The majority of the found nests (61% belong to genus Nannotrigona Cokerell, 1922. Nannotrigona mellaria was the specie with the greater nests number and higher population; Trigona (Tetragonisca angustula was found in all cemeteries, but in a smaller percentage that N. mellaria (29% of the total. In the Tena (Cundinamarca cemetery was found the nest highest density (118 nest/ha, with a tombs occupation percentage of 13.9%. We discussed the importance of cemeteries as an alternative for wild bees nesting sites conservation in urban areas.

  13. Multifemale nests and social behavior in Euglossa melanotricha (Hymenoptera, Apidae, Euglossini

    Directory of Open Access Journals (Sweden)

    Aline Andrade-Silva

    2012-05-01

    Full Text Available The nesting biology and social behavior of the euglossine bee species Euglossa melanotricha was analyzed based on the monitoring of eight nests found in man-made cavities and transferred to observation boxes. Euglossa melanotricha females usually construct their nests in cavities in the ground, in buildings, or in mounds. In this study, we present new data on the nesting biology of E. melanotricha. The process of reactivation of nests was commonly observed with one to three females participating in the reactivation. The duration of the process of reactivation ranged from 10 to 78 days (n = 31 and were longer during the rainy season. Time spent (in days for provisioning, oviposition and closing a single cell was higher in reactivations that occurred during the dry period.151 emergences were observed (39 males and 112 females. 90 (80.3% of the emerged females returned to the natal nest, but only 35 (38.9% remained and actively participated in the construction and provisioning of cells. The other 55 abandoned the nests after several days without performing any work in the nest. Matrifilial nest structure was regulated by dominance-subordinate aggressive behavior among females, where the dominant female laid almost all eggs. Task allocation was recognized by behavioral characteristics, namely, agonism and oophagy in cells oviposited by other females. Euglossa melanotricha is multivoltine and its nesting is asynchronous with respect to season. Our observations suggest a primitively eusocial organization. These observations of E. melanotricha provide valuable information for comparison with other species of Euglossa in an evolutionary context.

  14. Hive Relocation Does Not Adversely Affect Honey Bee (Hymenoptera: Apidae Foraging

    Directory of Open Access Journals (Sweden)

    Fiona C. Riddell Pearce

    2013-01-01

    Full Text Available Honey bees, Apis mellifera, face major challenges including diseases and reduced food availability due to agricultural intensification. Additionally, migratory beekeeping may subject colonies to a moving stress, both during the move itself and after the move, from the bees having to forage in a novel environment where they have no knowledge of flower locations. This study investigated the latter. We moved three colonies housed in observation hives onto the campus from a site 26 km away and compared their foraging performance to three similarly sized colonies at the same location that had not been moved. We obtained data on (1 foraging performance by calculating distance by decoding waggle dances, (2 hive foraging rate by counting forager departure rate, (3 forage quality by assessing sugar content of nectar from returning foragers, and (4 forager success by calculating the proportion of bees returning to the nest entrance with nectar in their crop. We repeated this 3 times (August 2010, October 2010, and June 2011 to encompass any seasonal effects. The data show no consistent difference in foraging performance of moved versus resident hives. Overall the results suggest that moving to a new location does not adversely affect the foraging success of honey bees.

  15. Effect of Maruca vitrata (Lepidoptera: Crambidae) host plants on life-history parameters of the parasitoid Apanteles taragamae (Hymenoptera: Braconidae)

    NARCIS (Netherlands)

    Dannon, A.E.; Tamo, M.; Agboton, C.; Huis, van A.; Dicke, M.

    2012-01-01

    The effect of four host plant species of the herbivore Maruca vitrata Fabricius (Lepidoptera: Crambidae) on development time, longevity, fecundity and sex ratio of the parasitoid Apanteles taragamae Viereck (Hymenoptera: Braconidae) was investigated under laboratory conditions. The larvae were

  16. A Total-Evidence Approach to Dating with Fossils, Applied to the Early Radiation of the Hymenoptera

    Science.gov (United States)

    Ronquist, Fredrik; Klopfstein, Seraina; Vilhelmsen, Lars; Schulmeister, Susanne; Murray, Debra L.; Rasnitsyn, Alexandr P.

    2012-01-01

    Abstract Phylogenies are usually dated by calibrating interior nodes against the fossil record. This relies on indirect methods that, in the worst case, misrepresent the fossil information. Here, we contrast such node dating with an approach that includes fossils along with the extant taxa in a Bayesian total-evidence analysis. As a test case, we focus on the early radiation of the Hymenoptera, mostly documented by poorly preserved impression fossils that are difficult to place phylogenetically. Specifically, we compare node dating using nine calibration points derived from the fossil record with total-evidence dating based on 343 morphological characters scored for 45 fossil (4--20 complete) and 68 extant taxa. In both cases we use molecular data from seven markers (∼5 kb) for the extant taxa. Because it is difficult to model speciation, extinction, sampling, and fossil preservation realistically, we develop a simple uniform prior for clock trees with fossils, and we use relaxed clock models to accommodate rate variation across the tree. Despite considerable uncertainty in the placement of most fossils, we find that they contribute significantly to the estimation of divergence times in the total-evidence analysis. In particular, the posterior distributions on divergence times are less sensitive to prior assumptions and tend to be more precise than in node dating. The total-evidence analysis also shows that four of the seven Hymenoptera calibration points used in node dating are likely to be based on erroneous or doubtful assumptions about the fossil placement. With respect to the early radiation of Hymenoptera, our results suggest that the crown group dates back to the Carboniferous, ∼309 Ma (95% interval: 291--347 Ma), and diversified into major extant lineages much earlier than previously thought, well before the Triassic. [Bayesian inference; fossil dating; morphological evolution; relaxed clock; statistical phylogenetics.] PMID:22723471

  17. Asociaciones áfido-parasitoide (Hemiptera: Aphididae; Hymenoptera: Braconidae, Aphidiinae en cultivos hortícolas orgánicos en Los Cardales, Buenos Aires, Argentina Aphid-parasitoid associations (Hemiptera: Aphididae; Hymenoptera: Braconidae, Aphidiinae on organic vegetable crops in Los Cardales, Buenos Aires, Argentina

    Directory of Open Access Journals (Sweden)

    Andrea V. Andorno

    2007-07-01

    Full Text Available Diez especies de áfidos (Hemiptera: Aphididae se hallaron parasitados por siete especies de parasitoides (Hymenoptera: Braconidae, Aphidiinae en cultivos hortícolas orgánicos. Myzus persicae (Sulzer fue el áfido más frecuentemente encontrado sobre una amplia variedad de cultivos, y con mayor diversidad de parasitoides asociados. Aphidius colemani Viereck fue el afidiino más usual, que ataca varias especies de áfidos. Ocho asociaciones tritróficas, involucrando Aphidius matricariae Haliday, han sido registradas por primera vez para la Argentina.Ten aphid species (Hemiptera: Aphididae were found parasitized by seven aphid parasitoid species (Hymenoptera: Braconidae, Aphidiinae on organic vegetable crops. Myzus persicae (Sulzer was the most frequent aphid found on a wide variety of crops, with the largest parasitoid diversity associated. Aphidius colemani Viereck was the most frequent aphidiine attacking several species of aphids. Eight tritrophic associations involving Aphidius matricariae Haliday are reported for the first time for Argentina.

  18. Temperature dependent functional response of Diaeretiella rapae (Hymenoptera: Aphidiidae) to the cabbage aphid, Brevicoryne brassicae (Hemiptera: Aphididae)

    DEFF Research Database (Denmark)

    Moayeri, Hamid R. S.; Madadi, Hossein; Pouraskari, Hossein

    2013-01-01

    Diaeretiella rapae MacIntosh (Hymenoptera: Aphidiidae) is one of the most common and successful parasitoids of the cabbage aphid. The functional response of D. rapae towards cabbage aphids was examined in laboratory studies at three constant temperatures, 17°C, 25°C and 30°C. D. rapae exhibited a...

  19. Visitantes florales diurnos del girasol (Helianthus annuus, Asterales: Asteraceae en la Argentina Diurnal floral visitors of sunflower (Helianthus annuus, Asterales: Asteraceae in Argentina

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    Juan P. Torretta

    2010-06-01

    Full Text Available El girasol (Helianthus annuus L. es un importante cultivo oleaginoso en la Argentina. Durante tres campañas agrícolas, se determinaron la diversidad y la abundancia del elenco de los visitantes florales diurnos de capítulos de girasol, en ocho sitios que cubren gran parte del área cultivada en Argentina. Setenta y seis morfo-especies de visitantes florales, pertenecientes a ocho órdenes, fueron capturados sobre capítulos de este cultivo. El principal orden fue Hymenoptera, con 37 especies o morfoespecies, de las cuales 32 fueron abejas (Apoidea. Las familias de abejas más representadas fueron Apidae (13, Megachilidae (11 y Halictidae (7. La abeja doméstica (Apis mellifera L. realizó el 93% de las visitas. La composición del elenco de visitantes no mostró un patrón de variación identificable a lo largo del día, ni con respecto a la distancia al borde del cultivo, pero varió entre sitios de muestreo. Se concluye que la abeja doméstica es el principal polinizador del girasol en la Argentina, aunque varias especies nativas de abejas (Melissodes tintinnans (Holmberg, M. rufithorax Brèthes, Melissoptila tandilensis Holmberg, y Megachile spp. podrían ser consideradas como potenciales polinizadores del cultivo.Sunflower (Helianthus annuus L. is an important oilseed crop in Argentina. During three agricultural years, the diversity and abundance of diurnal floral visitors of sunflower heads were determined in eight sites spanning much of this crop's cultivation area in Argentina. Seventysix morpho-species of floral visitors, belonging to eight orders, were captured on sunflower. The principal order was Hymenoptera, with 37 species or morpho-species, of which 32 were bees (Apoidea. The most represented bee families were Apidae (13, Megachilidae (11 and Halictidae (7. The domestic bee (Apis mellifera L. accounted for 93% of the visits. Floral visitor composition did not show an identifiable variation pattern either throughout the day or

  20. A new species of the Camponotus aureopilus VIEHMEYER, 1914 species-group (Hymenoptera: Formicidae) from Papua New Guinea

    Czech Academy of Sciences Publication Activity Database

    Shattuck, S.; Janda, Milan

    2009-01-01

    Roč. 12, - (2009), s. 251-253 ISSN 1994-4136 R&D Projects: GA MŠk LC06073; GA AV ČR KJB612230701 Grant - others:U.S. National Science Foundation(US) DEB-02-11591 Institutional research plan: CEZ:AV0Z50070508 Keywords : taxonomic description * Hymenoptera * Formicidae Subject RIV: EG - Zoology

  1. Ichneumonidae (Hymenoptera) parasitóides de larvas de Lepidoptera associadas a Croton floribundus Spreng (Euphorbiaceae)

    OpenAIRE

    Fernandes, Luciana Bueno dos Reis; Dias Filho, Manoel Martins; Fernandes, Marcelo Adorna; Penteado-Dias, Angelica Maria

    2010-01-01

    Parasitoids of the family Ichneumonidae (Hymenoptera) were obtained during an inventory of Lepidoptera larvae caught feeding in the wild on Croton floribundus (Euphorbiaceae). The Lepidoptera larvae were collected from host plants along trails inside three preserved forest areas in the Brazilian state of São Paulo. Fifteen different species of Ichneumonidae belonging to five subfamilies (Banchinae, Campopleginae, Cremastinae, Mesochorinae and Metopiinae) were obtained. Seven species of Ichneu...

  2. A hymenopterists’ guide to the Hymenoptera Anatomy Ontology: utility, clarification, and future directions

    Directory of Open Access Journals (Sweden)

    Katja Seltmann

    2012-05-01

    Full Text Available Hymenoptera exhibit an incredible diversity of phenotypes, the result of ~240 million years of evolution and the primary subject of more than 250 years of research. Here we describe the history, development, and utility of the Hymenoptera Anatomy Ontology (HAO and its associated applications. These resources are designed to facilitate accessible and extensible research on hymenopteran phenotypes. Outreach with the hymenopterist community is of utmost importance to the HAO project, and this paper is a direct response to questions that arised from project workshops. In a concerted attempt to surmount barriers of understanding, especially regarding the format, utility, and development of the HAO, we discuss the roles of homology, “preferred terms”, and “structural equivalency”. We also outline the use of Universal Resource Identifiers (URIs and posit that they are a key element necessary for increasing the objectivity and repeatability of science that references hymenopteran anatomy. Pragmatically, we detail a mechanism (the “URI table” by which authors can use URIs to link their published text to the HAO, and we describe an associated tool (the “Analyzer” to derive these tables. These tools, and others, are available through the HAO Portal website (http://portal.hymao.org. We conclude by discussing the future of the HAO with respect to digital publication, cross-taxon ontology alignment, the advent of semantic phenotypes, and community-based curation.

  3. Occurrence of Hymenoptera on Sus scrofa carcasses during summer and winter seasons in southeastern Brazil Ocorrência de Hymenoptera em carcaças de Sus scrofa durante as estações de inverno e verão do sudeste do Brasil

    Directory of Open Access Journals (Sweden)

    Leonardo Gomes

    2007-09-01

    Full Text Available Considerable importance has been given to nest construction and larval food transport to the nest as a precondition for the eusociality of insects. Most adult hymenopterans feed on liquids, although bees and a few wasps may also feed on pollen. Carrion represents an additional source of protein for some species and they will scavenge for dead animals in the wild. This paper aims at analyzing Hymenoptera visitors on a pig carcass during the process of decomposition, in the summer of 2005 and the winter of 2006 in Brazil, and comparing the results with other studies in the Neotropical region. To our knowledge, this is the first study which described the occurence of Agelaia pallipes, Polybia paulista and Scaptotrigona depilis on decomposing carcasses in southeastern Brazil. It also raises the hypothesis of possible applications of Hymenoptera to achieve more precise PMI estimations, apart from other insects already known as having great importance in such estimates.Considerável importância tem sido dada às construções de ninhos e transporte de alimento larval para o ninho como uma pré-condição para a eusociabilidade dos insetos. Muitos adultos de himenópteros alimentam-se em líquidos, embora as abelhas e poucas vespas podem também se alimentarem de pólen. Carcaças representam uma fonte adicional de proteína para algumas espécies e elas foram uma vez observadas se alimentando de animais mortos na natureza. Este trabalho tem por objetivo analisar Hymenoptera visitantes em carcaças de porcos durante o verão de 2005 e inverno de 2006 no Brasil, ao longo dos estágios de decomposição, comparando com resultados de outros estudos na região Neotropical. Pelo nosso conhecimento, esse é o primeiro estudo que descreveu a ocorrência de Agelaia pallipes, Polybia paulista e Scaptotrigona depilis em carcaças em decomposição no sudeste do Brasil. Isso também aumenta a hipótese na possibilidade de aplicação de Hymenoptera em auxiliar a

  4. Five new species of Meteorus Haliday (Hymenoptera: Braconidae: Euphorinae) from Brazil.

    Science.gov (United States)

    De Almeida, Luis Felipe Ventura; Dias, Angélica Maria Penteado

    2015-12-10

    Meteorus Haliday, 1835 (Hymenoptera: Braconidae) is a cosmopolitan genus with around 340 species described, all koinobiont endoparasitoids of Coleoptera or Lepidoptera larvae, and several of its hosts are pest insects. Previously to this work only two species were described from Brazil, M. eaclidis Muesebeck and M. townsendi Muesebeck. Five new species of Meteorus are here described: M. atlanticus n. sp., M. ferruginosus n. sp., M. itatiaiensis n. sp., M. monoceros n. sp., and M. strigatus n. sp. Three species are recorded for the first time from Brazil: M. jerodi Aguirre & Shaw, M. laphygmae Viereck and M. megalops Zitani.

  5. Biological parameters and thermal requirements of the parasitoid Praon volucre (Hymenoptera: Braconidae) with Macrosiphum euphorbiae (Hemiptera: Aphididae) as host

    NARCIS (Netherlands)

    Conti, De B.F.; Bueno, V.H.P.; Sampaio, M.V.; Lenteren, van J.C.

    2011-01-01

    The effect of temperature on the biology of Praon volucre (Haliday, 1833) (Hymenoptera: Braconidae) in Macrosiphum euphorbiae (Thomas, 1878) (Hemiptera: Aphididae) hosts was studied and the thermal requirements of the parasitoid were determined. Experiments were carried out at 16, 19, 22, 25, and 28

  6. Hymenoptera parasitoides associados às larvas de Lepidoptera em reflorestamento e sistemas agroflorestais da fazenda Canchim (Embrapa Pecuária Sudeste), São Carlos, São Paulo, Brasil

    OpenAIRE

    Aline Garcia Pereira

    2013-01-01

    Estudos da flora e fauna silvestres são importantes por contribuirem para a compreensão dos processos ecológicos que ocorrem em resposta às estratégias de manejo utilizadas. Os Hymenoptera são um grupo-chave para o estabelecimento de prioridades em conservação do ambiente, pois representam alta proporção da diversidade de insetos, sendo facilmente amostrados. O objetivo deste trabalho foi caracterizar a fauna de Hymenoptera parasitoides associada às larvas de Lepidoptera em área de refloresta...

  7. Identification of microsatellite markers for a worldwide distibuted, highly invasive ant species Tapinoma melanocephalum (Hymenoptera: Formicidiae)

    Czech Academy of Sciences Publication Activity Database

    Zima, Jan; Lebrasseur, O.; Borovanská, Michaela; Janda, Milan

    2016-01-01

    Roč. 113, JUNE 03 (2016), s. 409-414 E-ISSN 1802-8829 R&D Projects: GA ČR GAP505/12/2467 Grant - others:Marie Curie Fellowship(CZ) PIOFGA2009-25448 Institutional support: RVO:60077344 Keywords : Hymenoptera * Formicidae * Tapinoma melanocephalum Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.167, year: 2016 http://www.eje.cz/pdfs/eje/2016/01/53.pdf

  8. Utilização de recursos florais por abelhas (Hymenoptera, Apoidea em uma área de Caatinga (Itatim, Bahia, Brasil

    Directory of Open Access Journals (Sweden)

    Aguiar Cândida Maria Lima

    2003-01-01

    Full Text Available This study was designed to identify important food resource plants used by bee species in a Caatinga area, as well as describe the local patterns of floral use by bees. A total of 1,145 foraging bees, belonging to 60 species, were captured while visiting 50 plant species. Melochia tomentosa L., Sida galheirensis Ulbr., Erythroxylon catingae P. Cowan, and Ziziphus cotinifolia Reiss. were the most frequently visited plants. Melochia tomentosa, Solanum paniculatum L. and S. galheirensis were visited by larger number of bee species. Some oligolectic bees were identified. Apis mellifera Linnaeus, 1758 and Trigona spinipes (Fabricius, 1793 had the largest trophic niche breadth (2.71 and 2.31. The trophic niche overlap was highest (0.52 between Xylocopa grisescens Lepeletier, 1841 and Frieseomelitta silvestrii (Friese, 1902. The low trophic niche overlap between Apis mellifera and native stingless bees seems to be the result of intensive exploration of only a few flower sources by Africanized bees, not frequently visited by meliponids.

  9. A new species of Tamarixia Mercet (Hymenoptera, Eulophidae, parasitoid of Trioza aguacate Hollis & Martin (Hemiptera, Triozidae in Mexico

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    Zoya Yefremova

    2014-01-01

    Full Text Available Tamarixia aguacatensis Yefremova, sp. n. (Hymenoptera: Eulophidae: Tetrastichinae is described from Mexico as a parasitoid of the avocado psyllid, Trioza aguacate Hollis & Martin (Hemiptera: Triozidae. Trioza aguacate is a serious pest of avocado, Persea americana Miller. A key to the species of Tamarixia Mercet in Mexico is given.

  10. Phylogeny and population genetic structure of ant genus Acropyga (Hymenoptera: Formicidae) in Papua New Guinea

    Czech Academy of Sciences Publication Activity Database

    Janda, Milan; Matos Maravi, Pavel F.; Borovanská, Michaela; Zima, Jan; Youngerman, E.; Pierce, N. E.

    2016-01-01

    Roč. 30, č. 1 (2016), s. 28-40 ISSN 1445-5226 R&D Projects: GA ČR GAP505/12/2467 Grant - others:Marie Curie Fellowship(CZ) PIOFGA2009-25448; Operational Program Research and Development for Innovations(CZ) CZ.1.05/3.2.00/08.0144 Institutional support: RVO:60077344 Keywords : Acropyga * Hymenoptera * Papua New Guinea Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.172, year: 2016

  11. The parasites of cereal stem borers (Lepidoptera: Cossidae, Crambidae, Noctuidae, Pyralidae) in Africa, belonging to the family Braconidae (Hymenoptera: Ichneumonoidea)

    NARCIS (Netherlands)

    Achterberg, van C.; Polaszek, A.

    1996-01-01

    A review is given of the parasites (parasitoids) of the African cereal stem borers (including introduced species) belonging to the family Braconidae (Hymenoptera); 38 species belonging to 19 genera are keyed and treated. Three new species are described: Macrocentrus sesamivorus spec. nov. from

  12. New species of Megastylus (Hymenoptera: Ichneumonidae: Orthocentrinae) reared from larvae of Keroplatidae fungus gnats (Diptera) in a Dutch orchid greenhouse

    NARCIS (Netherlands)

    Humala, Andrei E.; Kruidhof, Marjolein; Woelke, Joop

    2017-01-01

    A new parasitoid wasp species belonging to the genus Megastylus (Hymenoptera: Ichneumonidae: Orthocentrinae) found in an orchid nursery in The Netherlands is described and illustrated: Megastylus woelkei sp. nov. It was reared from parasitized larvae of fungus gnats (Diptera: Keroplatidae). The

  13. The origins of species richness in the Hymenoptera: insights from a family-level supertree

    Directory of Open Access Journals (Sweden)

    Davis Robert B

    2010-04-01

    Full Text Available Abstract Background The order Hymenoptera (bees, ants, wasps, sawflies contains about eight percent of all described species, but no analytical studies have addressed the origins of this richness at family-level or above. To investigate which major subtaxa experienced significant shifts in diversification, we assembled a family-level phylogeny of the Hymenoptera using supertree methods. We used sister-group species-richness comparisons to infer the phylogenetic position of shifts in diversification. Results The supertrees most supported by the underlying input trees are produced using matrix representation with compatibility (MRC (from an all-in and a compartmentalised analysis. Whilst relationships at the tips of the tree tend to be well supported, those along the backbone of the tree (e.g. between Parasitica superfamilies are generally not. Ten significant shifts in diversification (six positive and four negative are found common to both MRC supertrees. The Apocrita (wasps, ants, bees experienced a positive shift at their origin accounting for approximately 4,000 species. Within Apocrita other positive shifts include the Vespoidea (vespoid wasps/ants containing 24,000 spp., Anthophila + Sphecidae (bees/thread-waisted wasps; 22,000 spp., Bethylidae + Chrysididae (bethylid/cuckoo wasps; 5,200 spp., Dryinidae (dryinid wasps; 1,100 spp., and Proctotrupidae (proctotrupid wasps; 310 spp.. Four relatively species-poor families (Stenotritidae, Anaxyelidae, Blasticotomidae, Xyelidae have undergone negative shifts. There are some two-way shifts in diversification where sister taxa have undergone shifts in opposite directions. Conclusions Our results suggest that numerous phylogenetically distinctive radiations contribute to the richness of large clades. They also suggest that evolutionary events restricting the subsequent richness of large clades are common. Problematic phylogenetic issues in the Hymenoptera are identified, relating especially to

  14. Insectos asociados con flores de malezas del Jardín Botánico de Santiago de Cuba, con énfasis en Hymenoptera

    Directory of Open Access Journals (Sweden)

    José L. Fernández T.

    2001-12-01

    Full Text Available Se estudiaron los insectos visitantes en flores de nueve especies de malezas del Jardín Botánico de Santiago de Cuba, Cuba Oriental, durante 1993 (marzo a junio, en primavera y 1994 (enero a marzo, final del invierno y comienzo de la primavera. Se contabilizaron unas 50 horas de recolecta en tres horarios diferentes (0900-0930 hr en 1993; 0900-0930 hr, 1200-1230 hr y 1500-1530 hr en 1994. Se identificaron más de 140 especies y por lo menos 37 familias; Hymenoptera fue el orden mejor representado (con más de la mitad de los individuos rerecolectados, seguido de Diptera, Coleoptera y Lepidoptera. Dentro de Hymenoptera las abejas (Apoidea fueron mayoritarias, especialmente Apis mellifera L.; seguidas de avispas (Vespidae, Pompilidae, Sphecidae e icneumónidos (Ichneumonidae; los microhimenópteros no se recolectaron. Se comparó la himenopterofauna en cuanto a diversidad, similitud, especies dominantes y subdominantes, preferencia de horarios, eficiencia de los muestreos, etc. Cada especie de planta sustentó una fauna característica. Casi un tercio de los himenópteros visitantes fueron controles biológicos de plagas agrícolas, y la mayoría pueden considerarse polinizadores. Especies afines filogenéticamente presentaron patrones de actividad diaria semejante. El horario 0900 -0930 hr fue el de mayor biodiversidad en cuanto a número de especies visitantes. Al analizar el número de especies con relación al número de especímenes rerecolectados, así como el total de especies y número de muestreos realizados, las funciones de mejor ajuste fueron polinomiales de segundo grado (r² = 0.9734 y r² = 0.9573 respectivamente, p The insect visitors of flowers in nine weeds species were studied in the Botanical Garden of Santiago de Cuba, Eastern Cuba, during 1993 (March - June, spring season and 1994 (January - March, end of winter and beginning of spring season. About 50 hours of collecting efforts were made at three times (0900-0930 hr in

  15. A new species of genus Chorebus Haliday (Hymenoptera, Alysiinae parasitising Hexomyza caraganae Gu (Diptera, Agromyzidae from NW China

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    Tao Li

    2017-03-01

    Full Text Available Chorebus (Stiphrocera hexomyzae sp. n. (Hymenoptera, Braconidae, Alysiinae, Dacnusini is described and illustrated. It was reared from twig galls of Hexomyza caraganae Gu (Diptera, Agromyzidae on Caragana korshinskii Kom. f. (Fabaceae in Ningxia and Inner Mongolia (NW China. A partial key to related or similar Chorebus species is provided.

  16. Effects of feeding frequency and sugar concentration on behavior and longevity of the adult aphid parasitoid: Aphidius ervi (Haliday) (Hymenoptera: Braconidae)

    NARCIS (Netherlands)

    Azzouz, H.; Giordanengo, P.; Wäckers, F.L.; Kaiser, L.

    2004-01-01

    Aphidius ervi (Haliday) (Hymenoptera: Braconidae) is a solitary aphid endoparasitoid. Adults feed on honeydew and possibly on other sugar sources such as nectar. Sugar sources can vary qualitatively and quantitatively according to biotic factors and environmental conditions. Experiments were

  17. Espectro polínico de mel de tiúba (Melipona fasciculata Smith, 1854, Hymenoptera, Apidae Pollen spectrum of honey of "tiúba" (Melipona fasciculata Smith, 1854, Hymenoptera, Apidae

    Directory of Open Access Journals (Sweden)

    Adriana Cecília Lima Martins

    2011-01-01

    Full Text Available O espectro polínico do mel de Melipona fasciculata Smith foi analisado com o objetivo de identificar os recursos nectaríferos utilizados por essa espécie. A identificação das plantas visitadas foi realizada com base na análise dos tipos polínicos encontrados em 12 amostras de mel coletadas, mensalmente, em uma colônia localizada no município de Palmeirândia, na área da Baixada Ocidental Maranhense (02º40'47,6S, 44º52'39,8"W, Brasil. As análises quantitativas e qualitativas foram realizadas com o objetivo de determinar as porcentagens e classes de frequência dos tipos polínicos presentes nas amostras de mel. Foram encontrados 45 tipos polínicos, sendo Pontederia parviflora Alexander (Pontederiaceae, espécie mais frequente em todo o período de amostragem (38,6%, pólen dominante em Outubro (86%, Junho (85%, Julho (76%, Agosto (49% e Setembro (51% e pólen acessório em Dezembro, Janeiro e Março. Mimosa caesalpiniifolia Benth (Mimosaceae foi a segunda espécie mais frequente (22,8% sendo pólen dominante em Novembro (46%, Abril (74% e Maio (72%. Myrcia eximia DC (Myrtaceae foi considerada pólen isolado importante. As famílias mais representativas no espectro polínico das amostras de mel foram Pontederiaceae e Mimosaceae. 50% dos méis foram biflorais, havendo também méis monoflorais (41,7% e heteroflorais (8,3%.The pollen spectrum of honey of the Melipona fasciculata was analyzed aiming to elucidate the nectariferous resources used by this species. The identification of the visited plants was based upon the analysis of the pollen spectrum of 12 honey samples collected in one meliponarium located in Palmeirândia county, lowland area of Maranhão State, Brazil (02º40'47,6S, 44º52'39,8"W. The quantitative and qualitative analysis of honey samples was conducted in order to determine the pollen type percentages and frequency classes. Forty-five pollen types were found, being Pontederia parviflora Alexander (Pontederiaceae the most frequent species during period of sampling (38.6% and considered dominant pollen in October (86%, June (85%, July (76%, August (49% and September (51% and accessory pollen in December, January and March. Mimosa caesalpiniifolia Benth (Mimosaceae was the second most frequent species (22.8% and was a dominant pollen in November (46%, April (74% and May (72%. Myrcia eximia DC. (Myrtaceae was considered important isolated pollen. The most representative families found in the pollen spectrum of the honey samples were Pontederiaceae and Mimosaceae. 50% of honey samples was bifloral, but there were also monofloral (41.7% and heterofloral (8.3% honeys.

  18. Comparative toxicities and synergism of apple orchard pesticides to Apis mellifera (L. and Osmia cornifrons (Radoszkowski.

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    David J Biddinger

    Full Text Available The topical toxicities of five commercial grade pesticides commonly sprayed in apple orchards were estimated on adult worker honey bees, Apis mellifera (L. (Hymenoptera: Apidae and Japanese orchard bees, Osmia cornifrons (Radoszkowski (Hymenoptera: Megachilidae. The pesticides were acetamiprid (Assail 30SG, λ-cyhalothrin (Warrior II, dimethoate (Dimethoate 4EC, phosmet (Imidan 70W, and imidacloprid (Provado 1.6F. At least 5 doses of each chemical, diluted in distilled water, were applied to freshly-eclosed adult bees. Mortality was assessed after 48 hr. Dose-mortality regressions were analyzed by probit analysis to test the hypotheses of parallelism and equality by likelihood ratio tests. For A. mellifera, the decreasing order of toxicity at LD₅₀ was imidacloprid, λ-cyhalothrin, dimethoate, phosmet, and acetamiprid. For O. cornifrons, the decreasing order of toxicity at LD₅₀ was dimethoate, λ-cyhalothrin, imidacloprid, acetamiprid, and phosmet. Interaction of imidacloprid or acetamiprid with the fungicide fenbuconazole (Indar 2F was also tested in a 1∶1 proportion for each species. Estimates of response parameters for each mixture component applied to each species were compared with dose-response data for each mixture in statistical tests of the hypothesis of independent joint action. For each mixture, the interaction of fenbuconazole (a material non-toxic to both species was significant and positive along the entire line for the pesticide. Our results clearly show that responses of A. mellifera cannot be extrapolated to responses of O.cornifrons, and that synergism of neonicotinoid insecticides and fungicides occurs using formulated product in mixtures as they are commonly applied in apple orchards.

  19. Ecologia da polinização da amoreira-preta (Rubus sp (Rosaceae em Timbó-SC, Brasil Pollination ecology of blackberry (Rubus sp. (Rosaceae in Timbo (SC, Brazil

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    Leônidas João de Mello Junior

    2011-09-01

    Full Text Available O presente trabalho verificou experimentalmente a ecologia da polinização da amoreira-preta (Rubus sp.. Objetivou-se confirmar a síndrome de polinização de Rubus sp., por meio de análise de seu sistema reprodutivo, quantificação da produção diária de néctar, levantamento da entomofauna que visita as flores da amoreira na área de estudo e análise do seu comportamento polinizador. Os experimentos foram realizados em uma área de cultivo de amoreira-preta, no mês de dezembro de 2005, município de Timbó (SC. Constatou-se que a maior taxa de frutificação (48,3 % ± 3,2 ocorreu via polinização livre, onde os insetos não foram excluídos. Foi verificada a ocorrência de autopolinização, porém com taxa inferior de frutificação (12,2% ± 4,9. A anemofilia não foi constatada na espécie. A avaliação da produção de néctar em Rubus sp. resultou em valores compatíveis com a síndrome de melitofilia. Os visitantes florais coletados e observados sobre as flores de Rubus sp. foram predominantemente abelhas da ordem Hymenoptera (97%, que iniciam, em menor número, a atividade de forrageamento às 8h, com pico de atividade às 12h e declinando até às 16h. As coletas resultaram numa amostragem de 1.360 abelhas, divididas em quatro famílias e 13 espécies. A família com maior riqueza de espécies (N = 7 foi Halictidae e a mais abundante foi Apidae, com 1.288 indivíduos. Em Apidae, houve o predomínio da espécie exótica Apis mellifera, cuja representação na amostra foi de 1.246 indivíduos. Os dados da abundância e as observações naturalísticas do seu comportamento da flor apontaram A. mellifera como o principal polinizador de Rubus sp., mas confirmam que as demais espécies de abelhas também participam na polinização de Rubus sp.The pollination ecology of the blackberry (Rubus sp. was studied by means of determining its reproductive system, the analysis of flower's nectar production. Sampling the insects that visit

  20. Hornets Have It: A Conserved Olfactory Subsystem for Social Recognition in Hymenoptera?

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    Antoine Couto

    2017-06-01

    Full Text Available Eusocial Hymenoptera colonies are characterized by the presence of altruistic individuals, which rear their siblings instead of their own offspring. In the course of evolution, such sterile castes are thought to have emerged through the process of kin selection, altruistic traits being transmitted to following generation if they benefit relatives. By allowing kinship recognition, the detection of cuticular hydrocarbons (CHCs might be instrumental for kin selection. In carpenter ants, a female-specific olfactory subsystem processes CHC information through antennal detection by basiconic sensilla. It is still unclear if other families of eusocial Hymenoptera use the same subsystem for sensing CHCs. Here, we examined the existence of such a subsystem in Vespidae (using the hornet Vespa velutina, a family in which eusociality emerged independently of ants. The antennae of both males and female hornets contain large basiconic sensilla. Sensory neurons from the large basiconic sensilla exclusively project to a conspicuous cluster of small glomeruli in the antennal lobe, with anatomical and immunoreactive features that are strikingly similar to those of the ant CHC-sensitive subsystem. Extracellular electrophysiological recordings further show that sensory neurons within hornet basiconic sensilla preferentially respond to CHCs. Although this subsystem is not female-specific in hornets, the observed similarities with the olfactory system of ants are striking. They suggest that the basiconic sensilla subsystem could be an ancestral trait, which may have played a key role in the advent of eusociality in these hymenopteran families by allowing kin recognition and the production of altruistic behaviors toward relatives.

  1. Dopluise (Hemiptera: Coccoidea geassosieer met die wipstertmier, Crematogaster peringueyi Emery (Hymenoptera: Formicidae

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    Johannes H. Giliomee

    2015-02-01

    Full Text Available Neste van die wipstertmier, Crematogaster peringueyi (Hymenoptera: Formicidae, is op verskeie plekke langs die kus van die Wes-Kaap versamel. Die doel was om vas te stel watter dopluisagtiges (Hemiptera: Coccoidea in die neste in assosiasie met hierdie miere leef. Dopluise van drie families, naamlik die Pseudococcidae (witluise, Coccidae (sagtedopluise en Kerriidae (lakdopluise is in die neste gevind, almal bekend daarvoor dat hulle heuningdou afskei. Hierdie mutualistiese verhouding tussen die miere en dopluise, bekend as mirmekofilie, is fakultatief van aard. Die wipstertmier blyk ook nie spesifiek te wees wat betref die plant waarop hulle nes maak nie.

  2. Improved sensitivity to venom specific-immunoglobulin E by spiking with the allergen component in Japanese patients suspected of Hymenoptera venom allergy.

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    Yoshida, Naruo; Hirata, Hirokuni; Watanabe, Mineaki; Sugiyama, Kumiya; Arima, Masafumi; Fukushima, Yasutsugu; Ishii, Yoshiki

    2015-07-01

    Ves v 5 and Pol d 5, which constitute antigen 5, are recognized as the major, most potent allergens of family Vespidae. Several studies have reported the diagnostic sensitivity of the novel recombinant (r)Ves v 5 and rPol d 5 allergens in routine clinical laboratory settings by analyzing a group of Vespula and Polistes venom-allergic patients. In this study, we analyzed the sensitivity to venom specific (s)IgE by spiking with rVes v 5 and rPol d 5 in Japanese patients suspected of Hymenoptera venom allergy. Subjects were 41 patients who had experienced systemic reactions to hornet and/or paper wasp stings. Levels of serum sIgE against hornet and paper wasp venom by spiking with rVes v 5 and rPold d 5, respectively, as improvement testing, compared with hornet and paper wasp venom, as conventional testing, were measured by ImmunoCAP. Of the 41 patients, 33 (80.5%) were positive (≥0.35 UA/ml) for hornet and/or paper wasp venom in conventional sIgE testing. sIgE levels correlated significantly (P venom (R = 0.78) in improvement testing and conventional testing. To determine specificity, 20 volunteers who had never experienced a Hymenoptera sting were all negative for sIgE against these venoms in both improvement and conventional testing. Improved sensitivity was seen in 8 patients negative for sIgE against both venoms in conventional testing, while improvement testing revealed sIgE against hornet or paper wasp venom in 5 (total 38 (92.7%)) patients. The measurement of sIgE following spiking of rVes v 5 and rPol d 5 by conventional testing in Japanese subjects with sIgE against hornet and paper wasp venom, respectively, improved the sensitivity for detecting Hymenoptera venom allergy. Improvement testing for measuring sIgE levels against hornet and paper wasp venom has potential for serologically elucidating Hymenoptera allergy in Japan. Copyright © 2015 Japanese Society of Allergology. Production and hosting by Elsevier B.V. All rights reserved.

  3. Structural Examination of the Dufour's Gland of the Cavity-nesting Bees Osmia lignaria Say and Megachile rotundata (Fabricius) (Hymenoptera: Megachilidae)

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    The Dufour’s gland of two solitary cavity-nesting bees, Osmia lignaria and Megachile rotundata (Hymenoptera: Megachilidae), were examined with microscopy to determine the structure and arrangement of the gland as part of the sting apparatus. The Dufour’s glands of these two bee species are similar ...

  4. The first record of the genus Tanaostigma (Hymenoptera: Tanaostigmatidae) in the Old World, with the description of a new species from India.

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    Gupta, Ankita; Joshi, Sunil

    2016-11-15

    Tanaostigma Howard (Hymenoptera: Tanaostigmatidae) is recorded for the first time in the fauna of the Old World, with T. indica Gupta sp. n.  described and illustrated from southern India, reared from Millettia (=Pongamia) pinnata (Fabaceae).

  5. Field-cage evaluation of the parasitoid Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) as a natural enemy of the coffee berry borer

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    Phymastichus coffea (Hymenoptera: Eulophidae) is an African parasitoid that has been imported to Mexico and other Latin American countries for the biological control of the coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae). As a part of the evaluation of this ...

  6. Larvae and Nests of Aculeate Hymenoptera (Hymenoptera: Aculeata) Nesting in Reed Galls Induced by Lipara spp. (Diptera: Chloropidae) with a Review of Species Recorded. Part II.

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    Astapenková, Alena; Heneberg, Petr; Bogusch, Petr

    2017-01-01

    The ability of aculeate Hymenoptera to utilize wetlands is poorly understood, and descriptions of their nests and developmental stages are largely absent. Here we present results based on our survey of hymenopterans using galls induced by Lipara spp. flies on common reed Phragmites australis in the years 2015-2016. We studied 20,704 galls, of which 9,446 were longitudinally cut and the brood from them reared in the laboratory, while the remaining 11,258 galls reared in rearing bags also in laboratory conditions. We recorded eight species that were previously not known to nest in reed galls: cuckoo wasps Chrysis rutilans and Trichrysis pumilionis, solitary wasps Stenodynerus chevrieranus and Stenodynerus clypeopictus, and bees Pseudoanthidium tenellum, Stelis punctulatissima, Hylaeus communis and Hylaeus confusus. Forty five species of Hymenoptera: Aculeata are known to be associated with reed galls, of which 36 make their nests there, and the other are six parasitoids of the family Chrysididae and three cuckoo bees of the genus Stelis. Of these species, Pemphredon fabricii and in southern Europe also Heriades rubicola are very common in reed galls, followed by Hylaeus pectoralis and two species of the genus Trypoxylon. We also found new host-parasite associations: Chrysis angustula in nests of Pemphredon fabricii, Chrysis rutilans in nests of Stenodynerus clypeopictus, Trichrysis pumilionis in nests of Trypoxylon deceptorium, and Stelis breviuscula in nests of Heriades rubicola. We provide new descriptions of the nests of seven species nesting in reed galls and morphology of mature larvae of eight species nesting in reed galls and two parasitoids and one nest cleptoparasite. The larvae are usually very similar to those of related species but possess characteristics that make them easy to distinguish from related species. Our results show that common reeds are not only expansive and harmful, but very important for many insect species associated with habitats

  7. The wasp larva's last supper: 100 million years of evolutionary stasis in the larval development of rhopalosomatid wasps (Hymenoptera: Rhopalosomatidae

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    V. Lohrmann

    2017-08-01

    Full Text Available Rhopalosomatidae are an unusual family of wasps (Hymenoptera: Aculeata comprising less than 100 species found in the tropics and subtropics of all continents except Europe and Antarctica. Whereas some species resemble nocturnal Ichneumonidae, others might be mistaken for spider wasps or different groups of brachypterous Hymenoptera. Despite their varied morphology, all members of the family supposedly develop as larval ectoparasitoids of crickets (Orthoptera: Grylloidea. Here, we report on the first record of a fossil rhopalosomatid larva which was discovered in mid-Cretaceous amber from northern Myanmar (Burma. The larva is attached to the lateral side of a cricket between the metafemur and the abdomen, impacting the natural position of the hind leg, exactly as documented for modern species. Additionally, the larval gestalt is strikingly similar to those of extant forms. These observations imply that this behavioral specialization, e.g., host association and positioning on host, likely evolved in the stem of the family at least 100 million years ago.

  8. Registro de nido de Camponotus rufipes (Formicidae: Hymenoptera en un armario metálico dentro de una estructura urbana | Nesting report of Camponotus rufipes (Formicidae: Hymenoptera in a metallic cabinet insight an urban structure

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    Cristina Sainz-Borgo

    2017-11-01

    Full Text Available Urban ants are common and have a great importance for humans, for the effects in the houses or for being vectors of pathogens. The present paper reports the presence of a nest of Camponotus atriceps (Formicidae: Hymenoptera inside a metal cabinet in a research laboratory at Simón Bolívar University (Caracas, Venezuela. This report constitutes one of the few records for this species in metallic structures, since they usually occupy wooden structures.

  9. The use of polliniferous resources by Melipona capixaba, an endangered stingless bee species.

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    Serra, Bruna Danielle Vieira; da Luz, Cynthia Fernandes Pinto; Campos, Lucio Antonio de Oliveira

    2012-01-01

    Abstract Pollen types present in samples from corbiculae of Melipona capixaba (Moure and Camargo) (Hymenoptera: Apidae: Meliponina) worker bees were analyzed, as well as pollen samples from food pots inside the hives in three sites located at the bees' original habitat. The aim was to find out the sources used as a trophic resource by this species. The dominant pollen grains in the spectrum of the samples belonged to the families Myrtaceae and Melastomataceae. Eucalyptus was the most frequent pollen type in the corbiculae in Conceição do Castelo municipality; Eucalyptus, Myrcia, and Melastomatacea/Combretaceae in the Fazenda do Estado district; and Eucalyptus and Myrcia in the São Paulo de Aracê district, both in the Domingos Martins municipality. Eucalyptus and Melastomataceae/Combretaceae were the predominant pollen types in the food pots. Eucalyptus was the most prevalent type all year round or most of the year. The most common pollen types in the months that Eucalyptus was not present or dominant in the samples were of remaining native forest species, "ruderal" (field) plants, fruit-bearing plants, and introduced ornamental plants.

  10. Impact of bifenthrin on honeybees and Culex quinquefasciatus.

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    Qualls, Whitney A; Xue, Rui-De; Zhong, He

    2010-06-01

    The impact of bifenthrin on honeybees, Apis mellifera (Hymenoptera: Apidae) was evaluated in both laboratory and semifield assays. Ten serial dilutions of bifenthrin and an acetone control using the bottle bioassay protocol were used in the laboratory to determine killing time after 15-, 30-, and 60-min honeybee exposure. Both dose and exposure time significantly affected honeybee mortality (df = 6, F = 10.9, P Bifenthrin was applied at 9.7 ml/liter, 19.5 ml/liter, and 29.5 ml/liter of water to common landscape vegetation, Melampodium paludosum Melanie (show star) and Duranta erecta L. (golden dewdrop); a water control was also used. Bee mortality was significantly higher (P < 0.05, df = 2, F = 20.8) at 29.5 ml/liter compared to the mortality at 19.5-ml/liter and 9.7-ml/liter application rates after 24-h exposure to the treated vegetation. Mortality of Culex quinquefasciatus exposed to treated vegetation was significantly (P < 0.05, df = 10, F = 114) different by week and by application rate.

  11. Inhibitory effect of indole analogs against Paenibacillus larvae, the causal agent of American foulbrood disease.

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    Alvarado, Israel; Margotta, Joseph W; Aoki, Mai M; Flores, Fernando; Agudelo, Fresia; Michel, Guillermo; Elekonich, Michelle M; Abel-Santos, Ernesto

    2017-09-01

    Paenibacillus larvae, a Gram-positive bacterium, causes American foulbrood (AFB) in honey bee larvae (Apis mellifera Linnaeus [Hymenoptera: Apidae]). P. larvae spores exit dormancy in the gut of bee larvae, the germinated cells proliferate, and ultimately bacteremia kills the host. Hence, spore germination is a required step for establishing AFB disease. We previously found that P. larvae spores germinate in response to l-tyrosine plus uric acid in vitro. Additionally, we determined that indole and phenol blocked spore germination. In this work, we evaluated the antagonistic effect of 35 indole and phenol analogs and identified strong inhibitors of P. larvae spore germination in vitro. We further tested the most promising candidate, 5-chloroindole, and found that it significantly reduced bacterial proliferation. Finally, feeding artificial worker jelly containing anti-germination compounds to AFB-exposed larvae significantly decreased AFB infection in laboratory-reared honey bee larvae. Together, these results suggest that inhibitors of P. larvae spore germination could provide another method to control AFB. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America.

  12. Inhibitory effect of gut bacteria from the Japanese honey bee, Apis cerana japonica, against Melissococcus plutonius, the causal agent of European foulbrood disease

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    Wu, Meihua; Sugimura, Yuya; Iwata, Kyoko; Takaya, Noriko; Takamatsu, Daisuke; Kobayashi, Masaru; Taylor, DeMar; Kimura, Kiyoshi; Yoshiyama, Mikio

    2014-01-01

    Abstract European foulbrood is a contagious bacterial disease of honey bee larvae. Studies have shown that the intestinal bacteria of insects, including honey bees, act as probiotic organisms. Microbial flora from the gut of the Japanese honey bee, Apis cerana japonica F. (Hymenoptera: Apidae) , were characterized and evaluated for their potential to inhibit the growth of Melissococcus plutonius corrig. (ex White) Bailey and Collins (Lactobacillales: Enterococcaceae) , the causative agent of European foulbrood. Analysis of 16S rRNA gene sequences from 17 bacterial strains isolated by using a culture-dependent method revealed that most isolates belonged to Bacillus, Staphylococcus, and Pantoea. The isolates were screened against the pathogenic bacterium M. plutonius by using an in vitro growth inhibition assay, and one isolate (Acja3) belonging to the genus Bacillus exhibited inhibitory activity against M. plutonius. In addition, in vivo feeding assays revealed that isolate Acja3 decreased the mortality of honey bee larvae infected with M plutonius, suggesting that this bacterial strain could potentially be used as a probiotic agent against European foulbrood. PMID:25368073

  13. A New Method for Quick and Easy Hemolymph Collection from Apidae Adults.

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    Grzegorz Borsuk

    Full Text Available Bio-analysis of insects is increasingly dependent on highly sensitive methods that require high quality biological material, such as hemolymph. However, it is difficult to collect fresh and uncontaminated hemolymph from adult bees since they are very active and have the potential to sting, and because hemolymph is rapidly melanized. Here we aimed to develop and test a quick and easy method for sterile and contamination-free hemolymph sampling from adult Apidae. Our novel antennae method for hemolymph sampling (AMHS, entailed the detachment of an antenna, followed by application of delicate pressure to the bee's abdomen. This resulted in the appearance of a drop of hemolymph at the base of the detached antenna, which was then aspirated using an automatic pipetter. Larger insect size corresponded to easier and faster hemolymph sampling, and to a greater sample volume. We obtained 80-100 μL of sterile non-melanized hemolymph in 1 minute from one Bombus terrestris worker, in 6 minutes from 10 Apis mellifera workers, and in 15 minutes from 18 Apis cerana workers (+/-0.5 minutes. Compared to the most popular method of hemolymph collection, in which hemolymph is sampled by puncturing the dorsal sinus of the thorax with a capillary (TCHS, significantly fewer bees were required to collect 80-100 μL hemolymph using our novel AMHS method. Moreover, the time required for hemolymph collection was significantly shorter using the AMHS compared to the TCHS, which protects the acquired hemolymph against melanization, thus providing the highest quality material for biological analysis.

  14. Resposta de fêmeas de Encarsia formosa Gahan (Hymenoptera: Aphelinidae aos odores do hospedeiro e da planta-hospedeira em olfatômetro de quatro vias Response of female Encarsia formosa Gahan (Hymenoptera: Aphelinidae to host and plant-host odors

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    Kátia Maria Medeiros de Siqueira

    2003-01-01

    Full Text Available A crescente importância da mosca-branca Bemisia tabaci raça B (Hemiptera: Aleyrodidae como praga agrícola tem incentivado a busca de inimigos naturais que possam ser utilizados em programas de controle biológico. Estudou-se a atração de fêmeas de Encarsia formosa (Hymenoptera: Aphelinidae aos odores emanados pelo seu hospedeiro - a mosca-branca B. tabaci raça B - em plantas de tomate, em olfatômetro de quatro vias. O parasitóide não apresentou atração aos odores da planta de tomate nem ao complexo planta de tomate-ninfas de B. tabaci.The increasing importance of the whitefly Bemisia tabaci race B (Hemiptera: Aleyrodidae as one of the major agricultural pest of this century, has resulted in a search for natural enemies that can be used in biological control programs. The response of naive females of Encarsia formosa (Hymenoptera: Aphelinidaeto volatiles from its hostspecies: insect (B. tabacci race B and plant (Lycopersicom esculentum Mill. were tested using 4-nose olfactometre. Parasitoid was not attracted by neither or insect hostspecies volatile.

  15. Differentially displayed expressed sequence tags in Melipona scutellaris (Hymenoptera, Apidae, Meliponini) development.

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    Santana, Flávia A; Nunes, Francis M F; Vieira, Carlos U; Machado, Maria Alice M S; Kerr, Warwick E; Silva, Wilson A; Bonetti, Ana Maria

    2006-03-01

    We have compared gene expression, using the Differential Display Reverse Transcriptase-Polymerase Chain Reaction (DDRT-PCR) technique, by means of mRNA profile in Melipona scutellaris during ontogenetic postembryonic development, in adult worker, and in both Natural and Juvenile Hormone III-induced adult queen. Six, out of the nine ESTs described here, presented differentially expressed in the phases L1 or L2, or even in both of them, suggesting that key mechanisms to the development of Melipona scutellaris are regulated in these stages. The combination HT11G-AP05 revealed in L1 and L2 a product which matches to thioredoxin reductase protein domain in the Clostridium sporogenes, an important protein during cellular oxidoreduction processes. This study represents the first molecular evidence of differential gene expression profiles toward a description of the genetic developmental traits in the genus Melipona.

  16. Occurrence and biogeographic aspects of Melipona quinquefasciata in NE Brazil (Hymenoptera, Apidae

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    LIMA-VERDE L. W.

    2002-01-01

    Full Text Available The stingless bee Melipona quinquefasciata is not included among the nine bee species of Melipona described in literature of NE Brazil. However, reports of some farmers raised suspicion on the occurrence of M. quinquefasciata in the state of Ceará, in NE Brazil. Investigations were carried out from July 1997 to September 2000, by means of trips to the areas of probable occurrence of this bee species. Results confirmed the presence of M. quinquefasciata in Ceará and determined its habitat along the chapada do Araripe (Araripe plateau and all extension of planalto da Ibiapaba (Ibiapaba plateau, in altitudes between 600 and 900 m. Melipona quinquefasciata lives in the phytocoenosis of cerrado (Brazilian savanna, cerradão (savanna forest and carrasco (montane deciduous shrub vegetation on the top of Araripe plateau, and only carrasco in the Ibiapaba plateau. Due to pressures caused by reduction of the area covered with native vegetation, large use of agrochemicals in anthropic areas and generalised predatory hunting of honey and beeswax, M. quinquefasciata is in risk of disappearing from the ecosystems of Araripe and Ibiapaba plateaus within a few years.

  17. Karyotypes and heterochromatin variation (C-bands in Melipona species (Hymenoptera, Apidae, Meliponinae

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    Rocha Marla Piumbini

    1998-01-01

    Full Text Available We describe the karyotypes of eight bee species of the genus Melipona and compare them in terms of heterochromatin content and location (C-banding technique. All species had 2n = 18 (females and n = 9 (males chromosomes, but a wide variation in heterochromatin content was detected among karyotypes. On the basis of these differences, the species were divided into two functional groups, one of them comprising species with a karyotype having a low heterochromatin content (M. bicolor bicolor, M. quadrifasciata, M. marginata, and M. asilvai, and the other species with a high heterochromatin content (M. seminigra fuscopilosa, M. capixaba, M. scutellaris, and M. captiosa. In the species with high heterochromatin content, heterochromatin occupied practically the entire extent of all chromosomes, with euchromatin being limited to the extremities, a fact that prevented observation of the centromere. In contrast, in the species with karyotypes having a low heterochromatin content, heterochromatin was visualized only in some chromosomes. In the chromosomes in which it was present, heterochromatin was located in the centromere or on the short arm. M. bicolor bicolor had the smallest heterochromatin content with only three chromosome pairs presenting heterochromatin in females. Increased heterochromatin content may be explained by interstitial and pericentromeric growth.

  18. Differentially displayed expressed sequence tags in Melipona scutellaris (Hymenoptera, Apidae, Meliponini development

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    Santana Flávia A.

    2006-01-01

    Full Text Available We have compared gene expression, using the Differential Display Reverse Transcriptase - Polymerase Chain Reaction (DDRT-PCR technique, by means of mRNA profile in Melipona scutellaris during ontogenetic postembryonic development, in adult worker, and in both Natural and Juvenile Hormone III-induced adult queen. Six, out of the nine ESTs described here, presented differentially expressed in the phases L1 or L2, or even in both of them, suggesting that key mechanisms to the development of Melipona scutellaris are regulated in these stages. The combination HT11G-AP05 revealed in L1 and L2 a product which matches to thioredoxin reductase protein domain in the Clostridium sporogenes, an important protein during cellular oxidoreduction processes. This study represents the first molecular evidence of differential gene expression profiles toward a description of the genetic developmental traits in the genus Melipona.

  19. Ultrastructure of the midgut endocrine cells in Melipona quadrifasciata anthidioides (Hymenoptera, Apidae

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    C. A. Neves

    Full Text Available In this study we describe the ultrastructure of the endocrine cells observed in the midgut of M. quadrifasciata anthidioides. This bee has two types of endocrine cells, which are numerous on the posterior midgut region. Cells of the closed type are smaller and have irregular secretory granules with lower electrondensity than those of the open cell type. The open cell type has elongated mitochondria mainly on the basal area, where most of the secretory granules are also found. Besides the secretion granules and mitochondria, endocrine cells in this species have well-developed autophagic vacuoles and Golgi complex elements.

  20. Antimicrobial, antioxidant and cytotoxic activities of propolis from Melipona orbignyi (Hymenoptera, Apidae).

    Science.gov (United States)

    Campos, Jaqueline Ferreira; dos Santos, Uilson Pereira; Macorini, Luis Fernando Benitez; de Melo, Adriana Mary Mestriner Felipe; Balestieri, José Benedito Perrella; Paredes-Gamero, Edgar Julian; Cardoso, Claudia Andrea Lima; de Picoli Souza, Kely; dos Santos, Edson Lucas

    2014-03-01

    Propolis from stingless bees is well known for its biologic properties; however, few studies have demonstrated these effects. Therefore, this study aimed to investigate the chemical composition and antimicrobial, antioxidant and cytotoxic activities of propolis from the stingless bee Melipona orbignyi, found in Mato Grosso do Sul, Brazil. The chemical composition of the ethanol extract of propolis (EEP) indicated the presence of aromatic acids, phenolic compounds, alcohols, terpenes and sugars. The EEP was active against the bacterium Staphylococcus aureus and the fungus Candida albicans. The EEP showed antioxidant activity by scavenging free radicals and inhibiting hemolysis and lipid peroxidation in human erythrocytes incubated with an oxidizing agent. Additionally, EEP promoted cytotoxic activity and primarily necrotic death in K562 erythroleukemia cells. Taken together, these results indicate that propolis from M. orbignyi has therapeutic potential for the treatment and/or prevention of diseases related to microorganism activity, oxidative stress and tumor cell proliferation. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. Agricultural Landscape and Pesticide Effects on Honey Bee (Hymenoptera: Apidae) Biological Traits.

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    Alburaki, Mohamed; Steckel, Sandra J; Williams, Matthew T; Skinner, John A; Tarpy, David R; Meikle, William G; Adamczyk, John; Stewart, Scott D

    2017-06-01

    Sixteen honey bee (Apis mellifera L.) colonies were placed in four different agricultural landscapes to study the effects of agricultural landscape and exposure to pesticides on honey bee health. Colonies were located in three different agricultural areas with varying levels of agricultural intensity (AG areas) and one nonagricultural area (NAG area). Colonies were monitored for their performance and productivity for one year by measuring colony weight changes, brood production, and colony thermoregulation. Palynological and chemical analyses were conducted on the trapped pollen collected from each colony and location. Our results indicate that the landscape's composition significantly affected honey bee colony performance and development. Colony weight and brood production were significantly greater in AG areas compared to the NAG area. Better colony thermoregulation in AG areas' colonies was also observed. The quantities of pesticides measured in the trapped pollen were relatively low compared to their acute toxicity. Unexplained queen and colony losses were recorded in the AG areas, while colony losses because of starvation were observed in the NAG area. Our results indicate that landscape with high urban activity enhances honey bee brood production, with no significant effects on colony weight gain. Our study indicates that agricultural crops provide a valuable resource for honey bee colonies, but there is a trade-off with an increased risk of exposure to pesticides. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  2. Pollination of Greenhouse Tomatoes by the Mexican bumblebee Bombus ephippiatus (Hymenoptera: Apidae

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    Carlos Hernan Vergara

    2012-04-01

    Full Text Available The Mexican native bumblebee Bombus ephippiatus Say was evaluated as a potential pollinator of greenhouse tomatoes (Solanum lycopersicon L.. The experiments were performed at San Andrés Cholula, Puebla, Mexico, from June to December 2004 in two 1 000 m2 greenhouses planted with tomatoes of the cultivar Mallory (Hazera ®. For the experiments, we used two colonies of Bombus ephippiatus, reared in the laboratory from queens captured in the field. Four treatments were applied to 20 study plants: pollination by bumble bees, manual pollination, pollination by mechanical vibration and no pollination (bagged flowers, no vibration. We measured percentage of flowers visited by bumble bees, number of seeds per fruit, maturing time, sugar content, fruit weight and fruit shape. All available flowers were visited by bumblebees, as measured by the degree of anther cone bruising. The number of seeds per fruit was higher for bumble bee-pollinated plants as compared with plants pollinated mechanically or not pollinated and was not significantly different between hand-pollinated and bumble bee-pollinated plants. Maturation time was significantly longer and sugar content, fresh weight and seed count were significantly higher for bumblebee pollinated flowers than for flowers pollinated manually or with no supplemental pollination, but did not differ with flowers pollinated mechanically.

  3. Workers dominate male production in the neotropical bumblebee Bombus wilmattae (Hymenoptera: Apidae

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    Vandame Rémy

    2011-06-01

    Full Text Available Abstract Background Cooperation and conflict in social insects are closely linked to the genetic structure of the colony. Kin selection theory predicts conflict over the production of males between the workers and the queen and between the workers themselves, depending on intra-colonial relatedness but also on other factors like colony efficiency, sex ratios, cost of worker reproduction and worker dominance behaviour. In most bumblebee (Bombus species the queen wins this conflict and often dominates male production. However, most studies in bumblebees have been conducted with only a few selected, mostly single mated species from temperate climate regions. Here we study the genetic colony composition of the facultative polyandrous neotropical bumblebee Bombus wilmattae, to assess the outcome of the queen-worker conflict over male production and to detect potential worker policing. Results A total of 120 males from five colonies were genotyped with up to nine microsatellite markers to infer their parentage. Four of the five colonies were queen right at point of time of male sampling, while one had an uncertain queen status. The workers clearly dominated production of males with an average of 84.9% +/- 14.3% of males being worker sons. In the two doubly mated colonies 62.5% and 96.7% of the male offspring originated from workers and both patrilines participated in male production. Inferring the mother genotypes from the male offspring, between four to eight workers participated in the production of males. Conclusions In this study we show that the workers clearly win the queen-worker conflict over male production in B. wilmattae, which sets them apart from the temperate bumblebee species studied so far. Workers clearly dominated male production in the singly as well the doubly mated colonies, with up to eight workers producing male offspring in a single colony. Moreover no monopolization of reproduction by single workers occurred.

  4. Nesting biology of four Tetrapedia species in trap-nests (Hymenoptera:Apidae:Tetrapediini

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    Evandro Camillo

    2005-06-01

    Full Text Available The nests used in this study were obtained from trap-nests (tubes of cardboard and cut bamboo stems placed on Santa Carlota Farm (Itaoca Section-IS, Santana Section-SS and Cerrado-Ce, Cajuru, SP, Brazil. The number of nests and corresponding species obtained were as follows: 516 nests of T. curvitarsis, 104 of T. rugulosa, 399 of T. diversipes and 98 of T. garofaloi. The most abundant species from SS and Ce was T.curvitarsis, and from IS it was T. diversipes. In general, most nests were collected during the hot and wet season (September to April. The nests were constructed with sand and an oily substance, and a single female established them. The cells were constructed in a linear series, sometimes followed by a vestibular cell. The number of brood cells ranged from 1 to 10 in T. curvitarsis (n=200, and in T. garofaloi (n=51, from 1 to 8 (n=30 in T. rugulosa, and from 1 to 6 (n=37 in T. diversipes. The pollen mass (pollen + oily substance contained a hollow, sometimes divided by a transverse ridge, on the exposed face of the pollen mass. The egg was vertically positioned in the lower part of the hollow. At times, the closing of a cell was initiated before provisioning was completed, with a construction of a collar at the cell limit. In some nests the final cellular partition also acted as a closure plug. Females began activities at 6:18 a.m.and ended between 3:31 and 6:26 p.m. Some females (T. curvitarsis , T. rugulosa and T. garofaloi did not spend the nights at their nests, returning to them only the following morning with additional material. In general, the development period (for males and females was greater in nests collected near the end of the hot and wet season than it was for nests collected in other months. Sex ratios for each species were as follows: T. curvitarsis, 1:1; T. rugulosa , 1.6:1 female; T. diversipes, 1.9:1; T. garofaloi, 2.8:1. Males and females of T. diversipes exhibited statistically similar sizes and in the other three species the females were larger than the males. The mortality rates were statistically similar: 33.2% for T. curvitarsis, 25.8% for T. rugulosa, 26.8% for T. diversipes and 38.2% for T. garofaloi. The parasitoids were: Coelioxoides exulans, Leucospis cayenensis, Anthrax sp., Coelioxys sp., Coelioxoides sp. and individuals of the family Meloidae. Rev. Biol. Trop.53(1-2:175-186. Epub 2005 Jun 24Los nidos usados en este estudio fueron obtenidos de nidos de trampa (tubos de cartón y tallos cortados de bambú localizados en la finca Santa Carlota (Itaoca Sección-IS, Santana Sección-SS y Cerrado-Ce, Cajuru, SP, Brasil. El número de nidos y las especies correpondientes obtenidas fueron:516 nidos de T. curvitarsis, 104 de T. rugulosa, 399 de T. diversipes y 98 de T. garofaloi. La especie más abundante en SS y Ce fue T. curvitarsis, y la de IS fue T.diversipes. En general, la mayoría de los nidos fueron recolectados durante la estación caliente y húmeda (septiembre a abril. Los nidos fueron construidos con arena y una sustancia aceitosa, y una hembra solitaria se estableció en ellos. Las celdas fueron contruidas en una serie lineal, algunas veces contiguo a una celda vestibular. El número de celdas amplias se distribuyó desde 1 a 10 en T. curvitarsis (n=200, y en T. garofaloi (n=51, de 1 a 8 (n=30 en T. rugulosa, y de 1 a 6 (n=37 en T. diversipes. Las masas de polen (polen más sustancia aceitosa contenían una depresión, algunas veces dividida por una carina transversal, sobre la cara expuesta de la masa de polen. Los huevos fueron puestos en la parte inferior de la depresión. A veces, el cierre de una celda fue iniciada antes de que el aprovicionamiento estuviera completo, con la construcción de un collar en el borde de la celda. En algunos nidos la partición final de las celdas también actuaba como un tapón. Las hembras empiezan sus actividades a las 6:18 a.m. y terminan entre 3:31 y 6:26 p.m. Algunas hembras (T. curvitarsis, T. rugulosa y T. garofaloi no pasan las noches en sus nidos, regresando a ellos solamente la mañana siguiente con material adicional. En general, el periodo de desarrollo (para machos y hembras fue mayor en nidos recolectados cerca del fin de la estación cálida y humeda que respecto a los nidos recolectados en otros meses. La proporción de sexos para cada especie fue: T. curvitarsis, 1:1; T. rugulosa, 1.6:1 hembras; T. diversipes, 1.9:1; T. garofaloi, 2.8:1. Machos y hembras de T. diversipes exhibieron tamaños estadísticamente similares y en las otras especies las hembras fueron más grandes que los machos. Las tasas de mortalidad fueron similares estadísticamente: 33.2% para T. curvitarsis, 25.8% para T. rugulosa, 26.8% para T. diversipes y 38.2% para T. garofaloi. Los parasitoides fueron Coelioxoides exulans, Leucospis cayenensis, Anthrax sp., Coelioxys sp., Coelioxoides sp.e individuos de la familia Meloidae

  5. Occurrence and biogeographic aspects of Melipona quinquefasciata in NE Brazil (Hymenoptera, Apidae

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    L. W. LIMA-VERDE

    Full Text Available The stingless bee Melipona quinquefasciata is not included among the nine bee species of Melipona described in literature of NE Brazil. However, reports of some farmers raised suspicion on the occurrence of M. quinquefasciata in the state of Ceará, in NE Brazil. Investigations were carried out from July 1997 to September 2000, by means of trips to the areas of probable occurrence of this bee species. Results confirmed the presence of M. quinquefasciata in Ceará and determined its habitat along the chapada do Araripe (Araripe plateau and all extension of planalto da Ibiapaba (Ibiapaba plateau, in altitudes between 600 and 900 m. Melipona quinquefasciata lives in the phytocoenosis of cerrado (Brazilian savanna, cerradão (savanna forest and carrasco (montane deciduous shrub vegetation on the top of Araripe plateau, and only carrasco in the Ibiapaba plateau. Due to pressures caused by reduction of the area covered with native vegetation, large use of agrochemicals in anthropic areas and generalised predatory hunting of honey and beeswax, M. quinquefasciata is in risk of disappearing from the ecosystems of Araripe and Ibiapaba plateaus within a few years.

  6. Cytogenetic characterization of two species of Frieseomelitta Ihering, 1912 (Hymenoptera, Apidae, Meliponini

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    Antônio F. Carvalho

    2011-01-01

    Full Text Available The cytogenetic analysis of Frieseomelitta dispar and F. francoi revealed the chromosome numbers 2n = 30 and n = 15 and a karyotypic formula 2K = 4M+2Mt+4A+20A M. The number of chromosomes observed was consistent with those reported for other Frieseomelitta species. The occurrence of the Mt chromosome and other features of the karyotype formulae suggest a close relationship between F. dispar, F. francoi and F. varia. Nevertheless, it was possible to differentiate the karyotypes of the species by DAPI/CMA3 staining, which revealed GC-rich regions on two chromosome pairs of F. dispar: one acrocentric and one pseudoacrocentric. In F. francoi, the same kinds of regions were observed on a pair of metacentrics and on a pair of acrocentrics. Our analysis also confirmed the chromosome number conservation in Frieseomelitta and suggests that infrequent pericentric inversion could constitute a synapomorphy for the group including F. dispar, F. francoi, and F. varia.

  7. Fluctuating asymmetry in Apis mellifera(Hymenoptera: Apidae as bioindicator of anthropogenic environments

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    Lorena Andrade Nunes

    2015-09-01

    Full Text Available The successful distribution of A. melliferais due to their ability to adjust to seasonal variations, considerable control over their internal physical environment and exploration of different resources. However, their populations have experienced different forms and levels of environmental pressure. This research aimed to verify the phenotypic plasticity in both size and shape of wings in A. melliferausing fluctuating asymmetry, based on geometric morphometrics from apiaries located in sites with high and low levels of anthropization. We sampled 16 locations throughout all five geographic regions of Brazil. At each site, samples were collected from 20 beehives installed in apiaries: 10 installed near high anthropogenic environments (Cassilàndia - MS, Fortaleza - CE, Maringá - PR, Aquidauana - MS, Rolim de Moura - RO, Riachuelo - SE, Ubirata - PR and Piracicaba - SP, and 10 in sites with low levels of human disturbance (Cassilàndia - MS, Itapiúna CE, Uniao da Vitoria - PR, Aquidauana - MS, Rolim de Moura - RO, Pacatuba - SE, Erval Seco - RS, Rio Claro - SP. A sample of 10 individuals was taken in each hive, totaling 200 per location, for a total of 1 600 individuals. We used fluctuating asymmetry (FA in size and shape of the forewing through geometric morphometrics. The FA analysis was conducted in order to check bilateral differences. The indexes of size and shape were submitted to analysis of variance (ANOVA, where the characters evaluated were used as factors to verify the size and shape differences. The results indicated an asymmetry on the shape of the wing (P < 0.001 but no asymmetry was observed on wing size. Considering FA as an environmental response and high and low impacted areas as a fixed factor, we observed significant differences (P < 0.05. The results for the wing shape in A. melliferademonstrated that this feature undergoes more variation during ontogeny compared to the variation in size. We concluded that bee samples collected from colonies with higher levels of human disturbance had higher wing-shape asymmetry; the variation of fluctuating asymmetry in the wing shape of honeybees can be used as an indicator of the degree of environmental anthropization.

  8. Foraging behavior of stingless bee Heterotrigona itama (Cockerell, 1918) (Hymenoptera : Apidae : Meliponini)

    Science.gov (United States)

    Jaapar, Mohd Fahimee; Jajuli, Rosliza; Mispan, Muhamad Radzali; Ghani, Idris Abd

    2018-04-01

    A study to investigate the foraging behavior of Heterotrigona itama (Cockerell, 1918) was conducted on three colonies between January 2016 and June 2016. A digital single-lens reflex (DSLR) with macro lens attached, and action camera (SJCAM) was used to record foraging behavior of H. itama in its colonies for 5 min per hour between 0800 to 1700 h for a day per 6 months. In addition, three data loggers (Watchdog B100 2K) has been installed adjacent to the observation nest for collect temperature and humidity in the study areas. Result showed that the numbers of return foragers was significantly different from January to June also with outgoing forager. The returning forager between hours showed significant different from 8 am to 5 pm also for outgoing forager. The ideal temperature related to foraging behavior for H. itama was 29°C to 32 °C Our finding also, helps to guide researcher to expand the knowledge in foraging behavior by stingless bee as well as encouraging more small farmers to start rearing at least for their own consumption. In addition, these findings also guide the farmers to manage their chemical toxic inside the meliponiculture.

  9. First successful case of in vitro fertilization-embryo transfer with venom immunotherapy for hymenoptera sting allergy

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    Tucker Michael J

    2004-10-01

    Full Text Available Abstract Background To describe immune and endocrine responses in severe hymenoptera hypersensitivity requiring venom immunotherapy (VIT during in vitro fertilization (IVF. Case presentation A 39-year old patient was referred for history of multiple miscarriage and a history of insect sting allergy. Four years earlier, she began subcutaneous injection of 100 mcg mixed vespid hymenoptera venom/venom protein every 5–6 weeks. The patient had one livebirth and three first trimester miscarriages. Allergy treatment was maintained for all pregnancies ending in miscarriage, although allergy therapy was discontinued for the pregnancy that resulted in delivery. At our institution ovulation induction incorporated venom immunotherapy (VIT during IVF, with a reduced VIT dose when pregnancy was first identified. Serum IgE was monitored with estradiol during ovulation induction and early pregnancy. Response to controlled ovarian hyperstimulation was favorable while VIT was continued, with retrieval of 12 oocytes. Serum RAST (yellow jacket IgE levels fluctuated in a nonlinear fashion (range 36–54% during gonadotropin therapy and declined after hCG administration. A healthy female infant was delivered at 35 weeks gestation. The patient experienced no untoward effects from any medications during therapy. Conclusion Our case confirms the safety of VIT in pregnancy, and demonstrates RAST IgE can remain

  10. Espectro polínico de amostras de mel de Melipona mandacaia Smith, 1863 (Hymenoptera: Apidae = Pollen spectrum from honey samples of Melipona mandacaia Smith, 1863 stingless bee (Hymenoptera: Apidae

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    Rogério Marcos de Oliveira Alves

    2006-01-01

    Full Text Available O espectro polínico de amostras de mel da abelha Melipona mandacaia foi analisado com objetivo de elucidar os recursos alimentares utilizados por essa espécie. A identificação das plantas visitadas foi realizada com base na análise dos tipos polínicos encontrados nas amostras de mel coletadas em 11 colônias localizadas no município de São Gabriel, em área de caatinga do Estado da Bahia, Brasil (11º14’S e 41º52’W. As análises quantitativas e qualitativas foram realizadas com o objetivo de determinar as porcentagens e classes de freqüência dos tipos polínicos presentes nas amostras de mel. Foram encontrados 26 tipos polínicos, sendo o tipo Piptadenia rigida (Mimosaceae considerado dominante. Ricinus communis (Euphorbiaceae, Mimosa verrucata (Mimosaceae e M. arenosa (Mimosaceae foram considerados pólen isolado importante. As famílias mais representativas no espectro polínico das amostras de mel foram Mimosaceae, Euphorbiaceae, Asteraceae e Anacardiaceae.The pollen spectrum from honey samples of Melipona mandacaia stingless bee was analyzed aiming at elucidating the alimentaryresources used by that species. The identification of the visited plants was based on the analysis of pollen from honey samples collected in 11 hives located in São Gabriel county, in the semiarid area of Bahia State, Brazil (11º14’S and 41º52’W. The quantitative and qualitative analyses of honey samples were conducted in order to determine the pollen types percentages and frequency classes. Twenty-six pollen types were found, being the Piptadenia rigida type (Mimosaceae considered dominant. Ricinus communis (Euphorbiaceae, Mimosa verrucata (Mimosaceae and M. arenosa (Mimosaceae were considered important isolated pollen. The most representative families found in the pollen spectrum of the honey samples were Mimosaceae, Euphorbiaceae, Asteraceae and Anacardiaceae.

  11. Primeiro registro de Eufriesea laniventris (Ducke, 1902 (Hymenoptera, Apidae, Euglossini no Amazonas, Brasil First record of Eufriesea laniventris (Ducke, 1902 (Hymenoptera, Apidae, Euglossini in the state of Amazonas, Brazil

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    Eliana Fernandez Storti

    2004-01-01

    Full Text Available É registrada pela primeira vez a ocorrência de Eufriesea laniventris no Amazonas. No ano 2000, foram coletados seis exemplares na região de Manaus (2º 36' S e 60º 02' W atraídos pelas substâncias odoríferas 1,8 cineol e salicilato de metila.The first record of Eufriesea laniventris in the state of Amazonas is here reported. Attracted to 1.8 cineole and methyl salicylate fragrances, six specimens were colected in the region of Manaus (2º 36' S 60º 02' W during the year 2000.

  12. L'activité de butinage des Apoides sauvages (Hymenoptera Apoidea sur les fleurs de maïs à Yaounde (Cameroon et réflexions sur la pollinisation des graminées tropicales

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    Tchuenguem-Fohouo F.N.

    2002-01-01

    Full Text Available The gatering activity of wild bees (Hymenoptera Apoidea on flowers of maize at Yaound (Cameroon and further considerations on pollination of the tropical Gramineae. At Nkolbisson (Yaounde, Cameroon, in May 1991, flowers of maize (Zea mays L; Poaceae were observed for the study of pollen gathering by five different species of wild bees: one Apidae Meliponinae (Dactylurina staudingeri and four Halictidae Nomiinae (Lipotriches andrei, Lipotriches langi, Lipotriches notabilis and Leuconomia granulata. Each of these Apoidea is well attracted by pollen of maize. The larger number of bees foraging at the same time on a panicle varies from one with Dactylurina staudingeri to four with Lipotriches andrei and Lipotriches notabilis. Generally, bees forage maize during the whole day and during the full flowering period but visits are more numerous in the morning and during the period of intense flowering. Median duration of a visit on a male spikelet varies from 1 sec with Dactylurina staudingeri to 7 sec with Lipotriches andrei. These Apoidea have an elaborated behaviour when gathering pollen of maize. Dactylurina staudingeri however seems less adapted to the floral morphology of Poaceae comparing with Lipotriches. When foraging maize, all these bees are regular visitors to flowers of this plant, even in the presence of other flower species in the vicinity of the crop. Bees studied have a positive impact on the yield of grains due to a complementary action with the well known one of the wind. The influence is indirect as the bees are seldom visiting the stigmates. The explanation is that when the bees are very common on the panicles they shake the anthers, inducing the release of pollen grains in the atmosphere even in the days without wind. The part of wild bees in the increase of yields is estimated to 3/ while the one of the domestic bees (Apis mellifera is estimated to 21/ in that locality. The authors are reviewing existing literature on grass crop

  13. Comparison of the olfactory preferences of four species of filth fly pupal parasitoid species (Hymenoptera: Pteromalidae) for hosts in equine and bovine manure

    Science.gov (United States)

    House flies (Musca domestica L.) and stable flies (Stomoxys calcitrans (L.)) (Diptera: Muscidae) are common pests in equine and cattle facilities. Pupal parasitoids primarily in the genera Spalangia and Muscidifurax (Hymenoptera: Pteromalidae) can be purchased for biological control of these flies. ...

  14. The Behavioural Abilities of Meteorus versicolor Wesm. and Rogas rossicus Kok. (Hymenoptera, Branconidae) Parasitoids of Tephrina arenacearia Den. Et Schiff. (Lepidoptera, Geometridae)

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    KERIMOVA, Ilhama Gudrat Gizi

    1998-01-01

    This paper concerns with the behavioural abilities of Meteorus versicolor Wesm. and Rogas rossicus Kok. (Hymenoptera, Braconidae)-internal parasitoids of Thephrina arenacearia Den. et Schiff. (Lepidoptera, Geometridae) as well. Periods of development phases and searching, ovipositional and mating behaviour of both parasitoids have been investigated.

  15. Mortality due to Hymenoptera stings in Costa Rica, 1985-2006 Mortalidad por picaduras de himenópteros en Costa Rica, 1985-2006

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    Mónica Prado

    2009-05-01

    Full Text Available OBJECTIVE: To analyze mortality due to Hymenoptera stings in Costa Rica during 1985-2006. METHODS: Records of deaths due to Hymenoptera stings in 1985-2006 were retrieved from Instituto Nacional de Estadística y Censos (National Statistics and Census Institute. Mortality rates were calculated on the basis of national population reports, as of 1 July of each year. Information for each case included age, gender, and the province in which the death occurred. In addition, reports of Hymenoptera sting accidents received by the Centro Nacional de Intoxicaciones (National Poison Center, CNI in 1995-2006 were obtained to assess exposure to these insects. RESULTS: Over the 22-year period analyzed, 52 fatalities due to Hymenoptera stings were recorded. Annual mortality rates varied from 0-1.73 per 1 million inhabitants, with a mean of 0.74 (95% confidence interval: 0.46-0.93. The majority of deaths occurred in males (88.5%, representing a male to female ratio of 7.7:1. A predominance of fatalities was observed in the elderly (50 years of age and older, as well as in children less than 10 years of age. The province with the highest mortality rate was Guanacaste. The CNI documented 1 591 reports of Hymenoptera stings (mostly by bees in 1995-2006, resulting in an annual average of 133 cases, with only a slight predominance of males over females (1.4:1. CONCLUSIONS: Stings by Hymenoptera, mostly by bees, constitute a frequent occurrence in Costa Rica that can be life-threatening in a small proportion of cases, most often in males and the elderly. The annual number of fatalities fluctuated from 0-6, averaging 2.4 deaths per year. Awareness should be raised not only among the general population, but also among health care personnel that should consider this risk in the clinical management of patients stung by Hymenoptera.OBJETIVO: Analizar la mortalidad por picaduras de himenópteros en Costa Rica en el período de 1985 a 2006. MÉTODOS: Se obtuvieron los

  16. Nota sobre o comportamento de agregação dos machos de Oxaea austera Gerstaecker (Hymenoptera, Apoidea, Oxaeinae na caatinga do Estado da Bahia, Brasil Notes on a male sleeping aggregation behavior of Oxaea austera Gerstaecker (Hymenoptera, Apoidea, Oxaeinae in the caatinga of Bahia State, Brazil

    Directory of Open Access Journals (Sweden)

    Favízia Freitas de Oliveira

    2002-03-01

    Full Text Available This note reports for the first time a "male sleeping aggregation" of the solitary bee Oxaea austera Gerstaecker, 1867 (Hymenoptera, Apoidea, Oxaeinae found near the town of Iaçú, Bahia, in Northeastern Brazil. This is also the first record of a species of Oxaea for the caatinga ecosystem.

  17. Taxonomic and faunistic study of Aulacidae (Hymenoptera, Evanioidea from Iran, with illustrated key to species

    Directory of Open Access Journals (Sweden)

    Mostafa Ghafouri Moghaddam

    2018-02-01

    Full Text Available Aulacidae are parasitoids of wood-boring larvae of Hymenoptera and Coleoptera, known in all zoogeographic regions of the World, except Antarctic. Two aulacids, Pristaulacus compressus (Spinola, 1808 and the rare Pristaulacus mourguesi Maneval, 1935, have been recently collected from Iran, the latter being a new record. Based on available data, the Iranian aulacid fauna includes five species within a single genus, Pristaulacus Kieffer 1900. A brief taxonomic treatment, as well as morphometric data and an illustrated key to species, are provided.

  18. Record of the genus Arrhenophagoidea Girault (Hymenoptera: Chalcidoidea: Encyrtidae from India, description of a new species from the Andaman Islands

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    M. Hayat

    2014-05-01

    Full Text Available The genus Arrhenophagoidea Girault (Hymenoptera: Encyrtidae is recorded for the first time from India and the Oriental region, and a new species, A. andamanica sp. Nov. is described from material collected in the Andaman and Nicobar Islands, India. As the genus is newly recorded from the Oriental region, a brief diagnosis is also given.

  19. Catalogue of Danish Alysiinae (Hymenoptera: Braconidae, with the description of two new species of Aspilota Foerster, 1863

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    Francisco Javier Peris-Felipo

    2016-12-01

    Full Text Available In the present study, a total of 153 species of Alysiinae (Hymenoptera: Braconidae from Denmark are catalogued. Two species are described as new for science: Aspilota leptoarticulata Munk & Peris-Felipo sp. nov. and A. grandis Munk & Peris-Felipo sp. nov. Additionally, 38 alysiine species are recorded for the first time for the Danish fauna. A faunistic list with distribution data and host records is provided.

  20. First discovery of the family Tanaostigmatidae (Hymenoptera: Chalcidoidea) from China with a description of a new gall-making species utilizing kudzu leaves

    Science.gov (United States)

    Yang Zhong-qi; Sun Jiang-hua; James P. Pitts

    2004-01-01

    A new species of Tanaostigmodes (Hymenoptera: Chalcidoidea, Tanaostigmatidae) is described from China- Tanaostigmodes puerariae sp. nov. This is the first record of this family in China. This new species has potential as a biological control agent for control of kudzu, Pueraria lobate, in the U.S., because its...

  1. The role of selected soil fauna as predators of Apethymus abdominalis Lep. (Hymenoptera: Tenthredinidae) in oak forests in the District Caiuti, Romania

    Science.gov (United States)

    C. Ciornei; N. Popa; L. Ciuca; C. Rang

    2003-01-01

    The present study was initiated in 2001 in the oak forests from Trotus valley (Forest District Caiucti - Bacau, Romania) which were heavily infested by oak sawflies Apethymus abdominalis Lep. (Hymenoptera: Tenthredinidae), in order to understand better the role of soil-inhabitating predators in population regulation of this pest.

  2. Egg parasitoids (Hymenoptera: Mymaridae and Trichogrammatidae) of the gall-making leafhopper Scenergates viridis (Hemiptera: Cicadellidae) from Uzbekistan, with taxonomic notes on the Palaearctic species of Aphelinoidea.

    Science.gov (United States)

    Rakitov, Roman; Triapitsyn, Serguei V

    2013-01-01

    A new species of the Aphelinoidea Girault (Hymenoptera: Trichogrammatidae), A. (Aphelinoidea) sariq Triapitsyn & Rakitov sp. n., is described from Uzbekistan. Both sexes were reared from eggs of the only known truly gall-making leafhopper, Scenergates viridis (Vilbaste), laid inside its galls on camelthorn, Alhagi maurorum Medikus; additional females were found dead inside the galls. Aphelinoidea sariq is the only known species of the nominate subgenus of Aphelinoidea whose body color is predominantly yellow. Taxonomic notes on other Palaearctic species of Aphelinoidea are provided; A. scythica Fursov, syn. n. is synonymized underA. (Aphelinoidea) turanica S. Trjapitzin. Another trichogrammatid, Par-acentrobia (Paracentrobia) sp., was reared from eggs of S. viridis in much smaller numbers. Also described from the same locality and host is Gonatocerus (Lymaenon) mitjaevi Triapitsyn & Rakitov sp. n. (Hymenoptera: Mymaridae).

  3. Pollinator diversity (Hymenoptera and Diptera in semi-natural habitats in Serbia during summer

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    Mudri-Stojnić Sonja

    2012-01-01

    Full Text Available The aim of this study was to assess species diversity and population abundance of the two main orders of pollinating insects, Hymenoptera and Diptera. The survey was conducted in 16 grassland fragments within agro-ecosystems in Vojvodina, as well as in surrounding fields with mass-flowering crops. Pollinators were identified and the Shannon-Wiener Diversity Index was used to measure their diversity. Five families, 7 subfamilies, 26 genera and 63 species of insects were recorded. All four big pollinator groups investigated were recorded; hoverflies were the most abundant with 32% of the total number of individuals, followed by wild bees - 29%, honeybees - 23% and bumblebees with 16%.

  4. Leaf-litter amount as a factor in the structure of a ponerine ants community (Hymenoptera, Formicidae, Ponerinae in an eastern Amazonian rainforest, Brazil

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    Alexandro Herbert dos Santos Bastos

    2011-12-01

    Full Text Available Leaf-litter amount as a factor in the structure of a ponerine ants community (Hymenoptera, Formicidae, Ponerinae in an eastern Amazonian rainforest, Brazil. Leaf-litter may be an important factor in structuring ponerine ant communities (Hymenoptera, Formicidae, Ponerinae in tropical rainforests. We specifically examined how leaf-litter affects the structure of a ponerine ant community in primary Amazonian rainforest sites at the Ferreira Penna Scientific Station, Pará, Brazil. A total of 53 species belonging to eight genera of three ponerine tribes were collected with mini-Winkler extractors. The amount of leaf-litter positively affected the abundance and richness of the ponerine ant community, and also influenced species composition. Nearby samples often had low species similarity, especially when adjacent samples differed in the amount of leaf-litter. Leaf-litter availability in Amazonian primary forests is a key factor for distribution of ground-dwelling ponerine species, even at small scales.

  5. A new species of Klabonosa Bouček (Hymenoptera: Pteromalidae) reared from eggs of Endochus sp. (Hemiptera: Reduviidae) from India.

    Science.gov (United States)

    Gupta, Ankita; Yeshwanth, H M; Sureshan, P M

    2018-04-23

    The genus Klabonosa Bouček (Hymenoptera: Pteromalidae) is recorded for the first time from the Oriental region, with both sexes of K. indica Gupta, Sureshan Yeshwanth sp. n. reared from eggs of the assassin bug Endochus sp. (Hemiptera: Reduviidae) on Artocarpus heterophyllus Lam. (Moraceae). The male is formally described and illustrated for the first time for the genus.

  6. Molecular phylogeny of Indo-Pacific carpenter ants (Hymenoptera: Formicidae, Camponotus) reveals waves of dispersal and colonization from diverse source areas

    Czech Academy of Sciences Publication Activity Database

    Clouse, R. M.; Janda, Milan; Blanchard, B.; Sharma, P.; Hoffmann, B. D.; Andersen, A. N.; Czekanski-Moir, J. E.; Krushelnycky, P.; Rabeling, C.; Wilson, E. O.; Economo, E. P.; Sarnat, E. M.; General, D. M.; Alpert, G. D.; Wheeler, W. C.

    2015-01-01

    Roč. 31, č. 4 (2015), s. 424-437 ISSN 0748-3007 R&D Projects: GA ČR GAP505/12/2467 Grant - others:Marie Curie Felloswhip(CZ) PIOFGA2009-25448 Institutional support: RVO:60077344 Keywords : Hymenoptera * Camponotus * molecular phylogeny Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 4.952, year: 2015 http://onlinelibrary.wiley.com/doi/10.1111/cla.12099/epdf

  7. First record of Platygaster luteipes Buhl (Hymenoptera Platygastridae) from leaf galls on black pepper along with first report of the species from India

    DEFF Research Database (Denmark)

    Anjana, M.; Rajmohana, K.; Buhl, Peter Neerup

    2016-01-01

    Platygaster luteipes Buhl (Hymenoptera: Platygastridae) is reported for the first time from India. Several specimens of both sexes of the species were reared from the globular galls on mature leaves of black pepper. The hitherto undescribed male of the species is characterized and illustrated....

  8. Review of the genus Craspedolcus Enderlein sensu lato in China, with the description of a new genus and four new species (Hymenoptera, Braconidae, Braconinae)

    NARCIS (Netherlands)

    Li, Y.; Achterberg, van C.; Chen, X.-x.

    2017-01-01

    A new genus is split off the genus Craspedolcus Enderlein, 1920 (Hymenoptera, Braconidae, Braconinae): Maculibracon gen. n. with type species Maculibracon abruptus sp. n. The genus Craspedolcus Enderlein sensu stricto is redefined, a key to both genera and to their species in China, Thailand and

  9. A New Species of Megastigmus Dalman (Hymenoptera: Torymidae) Reared from Seeds of Atlantic White Cedar (Cupressaceae), with Notes on Infestation Rates

    Science.gov (United States)

    J.J. Turgeon; K. Kamijo; G. DeBarr

    1997-01-01

    A new species, Megastigmus thyoides Kamijo (Hymenoptera: Torymidae), which emerged from seeds of Atlantic white cedar, Chamaecyparis thyoides (L.) B.S.P., collected in eastern United States is described and illustrated. This is the first record of this genus exploiting seeds of Cupressaceae in the Nearctic region. An average of 7% of the seeds collected from five sites...

  10. Additions to the fauna of Braconidae from Madeira and Selvagens Islands, with the description of five new species (Hymenoptera: Braconidae: Homolobinae, Alysiinae, Opiinae)

    NARCIS (Netherlands)

    Achterberg, van C.; Aguiar, Franquinho A.M.

    2009-01-01

    Twenty-one species of the family Braconidae (Hymenoptera) are added to the checklist of Braconidae from Madeira, resulting in 113 species, of which 17 species are endemic to Madeira Islands and 4 species are only known from Madeira and Canary Islands. Five species are reported new for the Selvagens

  11. Primera cita de Aleiodes laphygmae (Hymenoptera: Braconidae) para Argentina y de su asociación con larvas de Spodoptera eridania (Lepidoptera: Noctuidae)

    OpenAIRE

    Liliana VALVERDE; D. Carolina BERTA; Marcelo GERONIMO GOMEZ

    2012-01-01

    Se reporta por primera vez para Argentina Aleiodes laphygmae (Viereck) (Hymenoptera: Braconidae) y como parasitoide de larvas de Spodoptera eridania (Stoll) (Lepidoptera: Noctuidae), en plantaciones de soja en la provincia de Tucumán (Argentina). Se provee información biológica como hábitos, hospedadores y distribución.

  12. A new technique in the excavation of ground-nest bee burrows (Hymenoptera: Apoidea

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    Diego Marinho

    Full Text Available ABSTRACT Bees have a diversified natural history, thus the methods applied to study such diversity are varied. When it comes to studies of nesting biology, bees which nest in pre-existing cavities have been reasonably well studied since researchers started using trap-nests. However, bees whose nests are built underground are poorly studied due to the difficulty of finding natural nesting areas and the absence of a method that facilitates bee nest excavation. The latter is evidenced by the lack of accurate descriptions in literature of how nests are excavated. In this study we tested cylindrical rubber refills of eraser pen as a new material to be used as a tracer of underground nest galleries in a natural nesting area of two species of Epicharis Klug, 1807 (Apidae. We compared this technique directly with plaster in powder form mixed with water and our results with other methodological studies describing alternative methods and materials. The rubber refill technique overcame the main issues presented by materials such as plaster, molten metal alloys and bioplastic, namely: death of the organisms by high temperatures and/or formation of plugs and materials unduly following the roots inside the galleries.

  13. Oviposition behavior and survival of Tamarixia radiata (Hymenoptera: Eulophidae), an ectoparasitoid of the Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae), on hosts exposed to an entomopathogenic fungus

    Science.gov (United States)

    Antagonistic interactions between the nymphal parasitoid, Tamarixia radiata Waterston (Hymenoptera: Eulophidae), and the ARSEF 3581 isolate of the entomopathogenic fungus, Isaria fumosorosea Wize (Hypocreales: Cordycipitaceae) could disrupt biological control of the Asian citrus psyllid, Diaphorina ...

  14. A New Species of Solitary Meteorus (Hymenoptera: Braconidae) Reared from Caterpillars of Toxic Butterflies (Lepidoptera: Nymphalidae) in Ecuador

    OpenAIRE

    Shaw, Scott R.; Jones, Guinevere Z.

    2009-01-01

    A new species of parasitoid wasp, Meteorus rugonasus Shaw and Jones (Hymenoptera: Braconidae), is described from the Yanayacu Biological Station, Napo Province, Ecuador. The new species is diagnosed and compared to other species in the genus. It was reared from larvae of Pteronymia zerlina (Hewitson, 1855) (Lepidoptera: Nymphalidae, Ithomiinae) found feeding on leaves of Solanum (Solanaceae). The parasitoid is solitary. This is the first record of a Meteorus species attacking ithomiine Nympha...

  15. The genus Odontocynips Kieffer, 1910 (Hymenoptera, Cynipidae, Cynipini in Panama, with redescription of Cynips championi Cameron, 1883

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    Pujade-Villar, J.

    2011-06-01

    Full Text Available The genus Odontocynips Kieffer, 1910 (Hymenoptera, Cynipidae, Cynipini is recorded for the first time in Panama, including two species, O. championi (Cameron and O. hansoni Pujade-Villar, that induce galls on Quercus bumelioides Liebm. and Q. lancifolia Schledl & Cham. (Fagaceae, sect. Quercus, White Oaks, respectively. Odontocynips championi (Cameron, 1833, originally described as Cynips championi Cameron based solely on galls, is redescribed including the first description of the adults, a neotype is designated and a new combination is established. The known distribution and host range of O. hansoni, recorded earlier from Costa Rica, are also expanded upon.Se cita por primera vez para Panamá el género Odontocynips Kieffer, 1910 (Hymenoptera, Cynipidae, Cynipini, incluyendo dos especies: Odontocynips championi (Cameron y O. hansoni Pujade-Villar, que inducen agallas en Quercus bumelioides Liebm. y Q. lancifolia Schledl & Cham. (Fagaceae, sect. Quercus, robles blancos, respectivamente. Se redescribe Odontocynips championi (Cameron, 1833, descrita solo a partir de sus agallas como Cynips championi Cameron, se describen por primera vez los adultos, se designa un neotipo y se establece una nueva combinación taxonómica al transferirla al género Odontocynips. Por otra parte, se amplía la distribución geográfica y rango de hospedador de O. hansoni, previamente citada sólo de Costa Rica.

  16. Primera cita de Aleiodes laphygmae (Hymenoptera: Braconidae para Argentina y de su asociación con larvas de Spodoptera eridania (Lepidoptera: Noctuidae

    Directory of Open Access Journals (Sweden)

    Liliana VALVERDE

    2012-01-01

    Full Text Available Se reporta por primera vez para Argentina Aleiodes laphygmae (Viereck (Hymenoptera: Braconidae y como parasitoide de larvas de Spodoptera eridania (Stoll (Lepidoptera: Noctuidae, en plantaciones de soja en la provincia de Tucumán (Argentina. Se provee información biológica como hábitos, hospedadores y distribución.

  17. Ophioninae (Hymenoptera: Ichneumonidae wasp community in the cloudy forest Monteseco, Cajamarca, Peru

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    Evelyn Sánchez

    2014-12-01

    Full Text Available We describe the species composition of the subfamily Ophioninae (Hymenoptera: Ichneumonidae along an altitudinal gradient in the cloudy forest Monteseco, Cajamarca, Peru collected in 2009 and 2010. Eighteen species were recorded in three genera of Ophioninae: Alophophion, Enicospilus y Ophion. Five species are recorded for the first time in Peru: Ophion polyhymniae Gauld, 1988; Enicospilus cubensis (Norton, 1863; E. guatemalensis (Cameron, 1886; E. cressoni Hooker, 1912 y E. mexicanus (Cresson, 1874. Subfamily composition varies with the elevation. The highest species richness (S=11 was found at 2150 m and the lowest (S=3 at 3116 m. Enicospilus is more diverse from low to mid elevation, Ophion from mid to high elevation and Alophophion occurs predominantly at high elevation.

  18. Oogenesis pattern and type of ovariole of the parasitoid Palmistichus elaeisis (Hymenoptera: Eulophidae

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    Gilberto S. Andrade

    2012-09-01

    Full Text Available The knowledge on ovigeny in parasitoids is important for basic studies on physiology and applied biological control. The ovigeny pattern and type of ovariole of the parasitoid Palmistichus elaeisis Delvare & LaSalle (Hymenoptera: Eulophidae were studied in newly-emerged females at seven, 14, 24 and 48 h intervals after their emergence from Tenebrio molitor L. pupae (Coleoptera: Tenebrionidae. Females of P. elaeisis presented ovaries composed by four ovarioles of the meroistic polytrophic type. The yolk accumulation and chorionogenesis in P. elaeisis were concluded 24 h after the female emergence. The 48 h-old females show a high quantity of egg ready for oviposition. These findings can help to improve the mass production of P. elaeisis and the augmentative biological control by using this natural enemy.O conhecimento da ovigenia em parasitóides é importante para estudos básicos em fisiologia e para o controle biológico aplicado. O padrão de ovigenia e OVIGENY OF Palmistichus elaeisis (HYMENOPTERA: EULOPHIDAE o tipo de ovaríolo do parasitóide Palmistichus elaeisis Delvare & LaSalle (Hymenopera: Eulophidae foram estudados em fêmeas recém-emergidas e em intervalos de sete, 14, 24 de 48 horas após a emergência em pupas de Tenebrio molitor L. (Coleoptera: Tenebrionidae. Fêmeas de P. elaesis apresentaram o ovário composto por quatro ovaríolos do tipo meroístico politrófico. A deposição de vitelo e corionogênese em P. elaeisis foram concluídas 24 horas após a emergência. Fêmeas com 48 horas de idade apresentam grande quantidade de ovos prontos para a oviposição. Esses resultados podem ajudar a melhoria da produção massal de P. elaeisis e o controle biológico aplicado com esse parasitóide.

  19. Floral Preference of Melipona eburnea Friese (Hymenoptera: Apidae) in a Colombian Andean Region.

    Science.gov (United States)

    Obregon, D; Nates-Parra, G

    2014-02-01

    Melipona eburnea Friese is a stingless bee kept in some regions of Colombia, where it is reported to be vulnerable to extinction due to habitat disturbance. To contribute to raising conservation strategies, the aim of this study was to identify the floral preferences of this species using melissopalynological analysis. A total of 31 pollen pot samples and 37 honey samples were taken from March 2009 through March 2010 from four colonies in Fusagasuga, Colombia. We found 92 pollen types: 17 from pollen pot samples, 39 from honey samples (indicating the sources of nectar), and 36 in both types of samples. The most frequent pollen types in the pollen pot samples were Myrcia type (100%), Eucalyptus globulus (96.9%), and Fraxinus uhdei (96.9%). The most frequent pollen types in honey samples were E. globulus (97.4%) and Myrcia type (94.9%). The pollen types corresponded mainly to native plants (68%), trees (44.5%), plants whose sexual system is hermaphroditic (56.5%), and plants with inflorescences (76.2%). The most frequent shapes of the flowers were brush-like (type Myrtaceae) and dish-like (type Asteraceae), and the preferred flower colors were white or cream (52.2%). In general, we found that M. eburnea showed a strong preference for trees of the family Myrtaceae to obtain nectar and pollen, including native and introduced species. Some other families are contributing significantly, such as Melastomataceae for pollen collection and Asteraceae for nectar. These results highlight the key plant species for the diet of M. eburnea.

  20. Por que Melipona scutellaris (Hymenoptera, Apidae forrageia sob alta umidade relativa do ar?

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    Marília D. e Silva

    2011-06-01

    Full Text Available Há evidências de que a temperatura do ar e a umidade relativa afetam a atividade de voo de espécies de abelhas sociais Meliponini. Em particular, as espécies grandes do gênero Melipona Illiger, 1806 responderiam de maneira mais estreita à variação na umidade relativa. Neste estudo defende-se o argumento de que a umidade relativa seja uma variável de confusão. Nesta linha de argumentação, também foi analisado o papel da coleta de pólen sobre o ritmo diário de forrageio. A robusta Melipona scutellaris (Latreille, 1811 foi usada como modelo e a atividade diária de voo e de forrageio de pólen foi medida em 12 colônias (4 colônias/hábitat, em três tipos de hábitats, que variam principalmente quanto à pluviosidade, na área de distribuição natural desta espécie (Floresta Pluvial, Floresta Sazonal e Transição Floresta Tropical-Cerrados. A maioria da atividade de voo acontece durante a manhã. A atividade de forrageio das colônias foi mais elevada nas primeiras horas do alvorecer, quando a umidade relativa também era alta, frequentemente associada a picos de coleta de pólen. A atividade de voo decresceu abruptamente durante as temperaturas altas ao redor do meio dia. A relação da atividade de voo com a umidade relativa foi altamente significativa e linear, contrastando com a relação significativa e unimodal com a temperatura. Na relação com o forrageio de M. scutellaris, a umidade relativa se configura como uma variável contingente, em hábitats tropicais úmidos, considerando os padrões diários de variação do microclima e de forrageio de pólen. Este último padrão também sustenta a hipótese de partição temporal de fontes florais de pólen.

  1. Toxicity of Imidacloprid to the Stingless Bee Scaptotrigona postica Latreille, 1807 (Hymenoptera: Apidae).

    Science.gov (United States)

    Soares, Hellen Maria; Jacob, Cynthia Renata Oliveira; Carvalho, Stephan Malfitano; Nocelli, Roberta Cornélio Ferreira; Malaspina, Osmar

    2015-06-01

    The stingless bee Scaptotrigona postica is an important pollinator of native and cultivated plants in Brazil. Among the factors affecting the survival of these insects is the indiscriminate use of insecticides, including the neonicotinoid imidacloprid. This work determined the toxicity of imidacloprid as the topical median lethal dose (LD50) and the oral median lethal concentration (LC50) as tools for assessing the effects of this insecticide. The 24 and 48 h LD50 values were 25.2 and 24.5 ng of active ingredient (a.i.)/bee, respectively. The 24 and 48 h LC50 values were 42.5 and 14.3 ng a.i./µL of diet, respectively. Ours results show the hazard of imidacloprid and the vulnerability of stingless bees to it, providing relevant toxicological data that can used in mitigation programs to ensure the conservation of this species.

  2. Age-dependent attractivity of males’ sexual pheromones in Bombus terrestris (L.) [Hymenoptera, Apidae

    Czech Academy of Sciences Publication Activity Database

    Coppée, Audrey; Mathy, T.; Cammaerts, M.; Verheggen, F. J.; Terzo, M.; Iserbyt, S.; Valterová, Irena; Rasmont, P.

    2011-01-01

    Roč. 21, č. 2 (2011), s. 75-82 ISSN 0937-7409 R&D Projects: GA ČR GA203/09/1446 Institutional research plan: CEZ:AV0Z40550506 Keywords : Bombus terrestris * sexual pheromones * age-dependent variation * behavioural tests Subject RIV: CC - Organic Chemistry Impact factor: 1.556, year: 2011

  3. Nest structure and communal nesting in Euglossa (Glossura annectans Dressler (Hymenoptera, Apidae, Euglossini

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Garófalo

    1998-01-01

    Full Text Available Three nests of Euglossa (Glossura annectans Dressier, 1982 were obtained from trap nests at Serra do Japi, Jundiai, São Paulo State, Brazil. The bees nested in bamboo cane (one nest and in wooden-boxes (two nests. Solitary (two cases and pleometrotic (one case foundations were observed. Two nests were re-used once by two females working in each of them. Re-using females that shared the nests were of the same generation and each built, provisioned and oviposited in her own cells, characterizing a communal association. The brood development period was related to climatic conditions. Natural enemies included Anthrax oedipus oedipus Fabricius, 1805 (Bombyliidae, Coelioxys sp. (Megachilidae and Melittobia sp. (Eulophidae.

  4. Taxonomic notes on stingless bee Trigona (Tetragonula iridipennis Smith (Hymenoptera: Apidae from India

    Directory of Open Access Journals (Sweden)

    K. Vijayakumar

    2014-10-01

    Full Text Available Stingless Bees are a large and diverse taxon and more than 500 species are recorded worldwide.  Based on the previous reports, totally seven species are reported from India.  The present study reports male and female morphological characteristics of Tetragonula iridipennis belonging to Tetragonula subgenus in Nellithurai Village, Tamil Nadu, India.  The species of Tetragonula group are extremely similar in the external morphology of the workers. The glabrous bands on mesoscutum of female bees and structure and arrangement of gonostylus and the penis valve of male bees are used to separate T. iridipennis from other species in this group.  The identity of T. iridipennis is made based on the male and female key morphological characteristics in the previous literature.

  5. Effects of clothianidin on Bombus impatiens (Hymenoptera: Apidae) colony health and foraging ability.

    Science.gov (United States)

    Franklin, Michelle T; Winston, Mark L; Morandin, Lora A

    2004-04-01

    We conducted laboratory experiments to investigate the lethal and sublethal effects of clothianidin on bumble bee, Bombus impatiens Cresson, colony health and foraging ability. Bumble bee colonies were exposed to 6 ppb clothianidin, representing the highest residue levels found in field studies on pollen, and a higher dose of 36 ppb clothianidin in pollen. Clothianidin did not effect pollen consumption, newly emerged worker weights, amount of brood or the number of workers, males, and queens at either dose. The foraging ability of worker bees tested on an artificial array of complex flowers also did not differ among treatments. These results suggest that clothianidin residues found in seed-treated canola and possibly other crops will not adversely affect the health of bumble bee colonies or the foraging ability of workers.

  6. Orchid bee (Hymenoptera: Apidae community from a gallery forest in the Brazilian Cerrado

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    Francinaldo S Silva

    2012-06-01

    Full Text Available The orchid bees are a very important group of pollinators distributed in the Neotropics. Although a lot of studies concerning male euglossine bees have been done in this region, few works have so far been carried out in the Cerrado biome. This manuscript has the main objective to present the orchid bee community from a Gallery Forest in the Northeastern Brazilian Cerrado landscape, taking account the species composition, abundance, seasonality and hourly distribution. Male euglossine bees were collected monthly from October 2007 to May 2009, in the Reserva Florestal da Itamacaoca belonging to the Companhia de Água e Esgoto do Maranhão, in Chapadinha municipality, Maranhão State. The scents eucalyptol, eugenol and vanillin were utilized, between 07:00 and 17:00hr, to attract the euglossine males. Cotton balls were dampened with the scents and suspended by a string on tree branches 1.5m above soil level, set 8m from one another. The specimens were captured with entomological nets, killed with ethyl acetate and transported to the laboratory to be identified. A total of 158 individuals and 14 species of bees were recorded. The genus Eulaema was the most representative group of euglossine bees in relation to the total number of the sampled individuals, accounting for 50.6% of bees followed by Euglossa (26.6%, Eufriesea (15.2% and Exaerete (7.6%. The most frequent species were Eulaema nigrita (27.8%, Eulaema cingulata (19% and Euglossa cordata (18.3%. Many species typical of forested environments were found in samples, like Euglossa avicula, Euglossa violaceifrons and Eulaema meriana, emphasizing the role played by the Gallery Forests as bridge sites to connect the two great biomes of Amazonia and Atlantic Forest. The occurrence of Exaerete guaykuru represents the second record of this species for the Neotropical region, and both records coming from the Gallery Forest zones. The male euglossine bees were sampled mainly in the dry season, where 62.5% of the individuals were collected in that period. Eufriesea species appeared at the baits only in the wet season. The hourly frequency of bees at scent baits showed a clear preference for the morning period, where 87.9% visited the baits from 07:00 to 12:00hr. The euglossine bee fauna found in the Northeastern Maranhão Cerrado is represented chiefly by species of large geographic distribution and by some forest bee species, where their occurrence is maybe related to to the environmental conditions supported by the Gallery Forest ecosystem. Rev. Biol. Trop. 60 (2: 625-633. Epub 2012 June 01.Las abejas de las orquídeas son un grupo muy importante de polinizadores distribuido en el Neotrópico, incluyendo Brasil. Aunque una gran cantidad de estudios sobre las abejas euglosinas se han hecho en esa región, pocos trabajos han sido hasta ahora llevado a cabo en el bioma del Cerrado. Este manuscrito tiene como objetivo principal mostrar la comunidad de abejas de orquídeas de un bosque de galería en el Cerrado brasileño, así como la composición de las especies, abundancia, estacionalidad y distribución horaria. Abejas euglosinas fueron capturadas mensualmente desde octubre 2007 hasta mayo 2009 en la Reserva Forestal da Itamacaoca perteneciente a la Companhia de Água e Esgoto do Maranhão, en el municipio de Chapadinha, Maranhão, Brasil. Eucaliptol, eugenol y vainillina se utilizaron para atraer las abejas, entre las 07:00 y 17:00hr. Bolas de algodón fueron impregnadas con los respectivos compuestos aromáticos y suspendidas por un cordel en ramas de los árboles alrededor de 1.5m sobre el nivel del suelo, separados 8m entre sí. Los especímenes fueron capturados con redes entomológicas, fijados con acetato de etilo y transportados al laboratorio para su identificación. Un total de 158 individuos y 14 especies de abejas fueron registrados. El género Eulaema fue el grupo más representativo de las abejas euglosinas, lo que representa el 50.6% de las abejas seguido por Euglossa (26.6%, Eufriesea (15.2% y Exaerete (7.6%. Las especies más frecuentes fueron: Eulaema nigrita (27.8%, cingulata Eulaema (19% y Euglossa cordata (18.3%. Muchas especies típicas de los ambientes forestales fueron capturadas, como Euglossa avicula, Euglossa violaceifrons y Eulaema meriana. La aparición de Exaerete guaykuru representa el segundo registro de esta especie para la región Neotropical, y ambos registros procedentes de las zonas de bosque de galería. Las abejas euglosinas fueron recolectadas principalmente en la estación seca (62.5%, pero el género Eufriesea apareció sobre todo en la estación lluviosa. Las abejas mostraron una clara preferencia por el período de la mañana, donde el 87.9% visitó los cebos aromáticos entre 07:00 y las 12:00hr. La fauna de abejas euglosinas está representada principalmente por especies de distribución geográfica amplia y por algunas especies forestales. La aparición de estas especies puede deberse a las condiciones ambientales presentes en el bosque de galería.

  7. Expression of varroa sensitive hygiene (VSH) in commercial VSH honey bees (Hymenoptera: Apidae)

    Science.gov (United States)

    We tested six commercial sources of honey bees (Apis mellifera L.) that were bred to include the trait of varroa sensitive hygiene (VSH). VSH confers resistance to the parasitic mite Varroa destructor Anderson & Trueman. Queens from these sources were established in colonies which later were measure...

  8. Proteomic analysis in the Dufour's gland of Africanized Apis mellifera workers (Hymenoptera: Apidae.

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    Aparecida das Dores Teixeira

    Full Text Available The colony of eusocial bee Apis mellifera has a reproductive queen and sterile workers performing tasks such as brood care and foraging. Chemical communication plays a crucial role in the maintenance of sociability in bees with many compounds released by the exocrine glands. The Dufour's gland is a non-paired gland associated with the sting apparatus with important functions in the communication between members of the colony, releasing volatile chemicals that influence workers roles and tasks. However, the protein content in this gland is not well studied. This study identified differentially expressed proteins in the Dufour's glands of nurse and forager workers of A. mellifera through 2D-gel electrophoresis and mass spectrometry. A total of 131 spots showed different expression between nurse and forager bees, and 28 proteins were identified. The identified proteins were categorized into different functions groups including protein, carbohydrate, energy and lipid metabolisms, cytoskeleton-associated proteins, detoxification, homeostasis, cell communication, constitutive and allergen. This study provides new insights of the protein content in the Dufour's gland contributing to a more complete understanding of the biological functions of this gland in honeybees.

  9. Lethal and sublethal effects of azadirachtin on the bumblebee Bombus terrestris (Hymenoptera: Apidae).

    Science.gov (United States)

    Barbosa, Wagner Faria; De Meyer, Laurens; Guedes, Raul Narciso C; Smagghe, Guy

    2015-01-01

    Azadirachtin is a biorational insecticide commonly reported as selective to a range of beneficial insects. Nonetheless, only few studies have been carried out with pollinators, usually emphasizing the honeybee Apis mellifera and neglecting other important pollinator species such as the bumblebee Bombus terrestris. Here, lethal and sublethal effects of azadirachtin were studied on B. terrestris via oral exposure in the laboratory to bring out the potential risks of the compound to this important pollinator. The compound was tested at different concentrations above and below the maximum concentration that is used in the field (32 mg L(-1)). As most important results, azadirachtin repelled bumblebee workers in a concentration-dependent manner. The median repellence concentration (RC50) was estimated as 504 mg L(-1). Microcolonies chronically exposed to azadirachtin via treated sugar water during 11 weeks in the laboratory exhibited a high mortality ranging from 32 to 100 % with a range of concentrations between 3.2 and 320 mg L(-1). Moreover, no reproduction was scored when concentrations were higher than 3.2 mg L(-1). At 3.2 mg L(-1), azadirachtin significantly inhibited the egg-laying and, consequently, the production of drones during 6 weeks. Ovarian length decreased with the increase of the azadirachtin concentration. When azadirachtin was tested under an experimental setup in the laboratory where bumblebees need to forage for food, the sublethal effects were stronger as the numbers of drones were reduced already with a concentration of 0.64 mg L(-1). Besides, a negative correlation was found between the body mass of male offspring and azadirachtin concentration. In conclusion, our results as performed in the laboratory demonstrated that azadirachtin can affect B. terrestris with a range of sublethal effects. Taking into account that sublethal effects are as important as lethal effects for the development and survival of the colonies of B. terrestris, this study confirms the need to test compounds on their safety, especially when they have to perform complex tasks such as foraging. The latter agrees with the recent European Food Safety Authority guidelines to assess 'potentially deleterious' compounds for sublethal effects on behavior.

  10. Species of Euglossa of the analis group in the Atlantic forest (Hymenoptera, Apidae

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    Luiz R. R. Faria

    2012-08-01

    Full Text Available The species of Euglossa Latreille, 1802 of the analis group inhabiting the Brazilian Atlantic forest are revised and identification keys for males and females are provided. Five species are recognized in the Atlantic forest: Euglossa cognata Moure, 1970, Euglossa marianae Nemésio, 2011, Euglossa roderici Nemésio, 2009 and two new species described here, Euglossa botocuda sp. nov. and Euglossa calycina sp. nov. These two new species have been misidentified by previous authors as Atlantic forest populations of, respectively, Euglossa iopyrrha Dressler, 1982 and Euglossa mixta Friese, 1899. Relevant morphological features are illustrated and distribution maps are also provided. Notes on the analis group are included and an additional available name, Euglossa aureiventris Friese, 1899, is placed in this species group.

  11. Drifting bumble bee (Hymenoptera: Apidae) workers in commercial greenhouses may be social parasites

    NARCIS (Netherlands)

    Birmingham, A.L.; Hoover, S.E.; Winston, M.L.; Ydenberg, R.C.

    2004-01-01

    Commercial greenhouses require high densities of managed bumble bee (Bombus occidentalis Greene, 1858 and Bombus impatiens Cresson, 1863) colonies to pollinate crops such as tomatoes (Lycopersicon esculentum Miller). We examined drifting, a behavioural consequence of introducing closely aggregated

  12. Proteomic analysis in the Dufour's gland of Africanized Apis mellifera workers (Hymenoptera: Apidae).

    Science.gov (United States)

    Teixeira, Aparecida das Dores; Games, Patricia D; Katz, Benjamin B; Tomich, John M; Zanuncio, José C; Serrão, José Eduardo

    2017-01-01

    The colony of eusocial bee Apis mellifera has a reproductive queen and sterile workers performing tasks such as brood care and foraging. Chemical communication plays a crucial role in the maintenance of sociability in bees with many compounds released by the exocrine glands. The Dufour's gland is a non-paired gland associated with the sting apparatus with important functions in the communication between members of the colony, releasing volatile chemicals that influence workers roles and tasks. However, the protein content in this gland is not well studied. This study identified differentially expressed proteins in the Dufour's glands of nurse and forager workers of A. mellifera through 2D-gel electrophoresis and mass spectrometry. A total of 131 spots showed different expression between nurse and forager bees, and 28 proteins were identified. The identified proteins were categorized into different functions groups including protein, carbohydrate, energy and lipid metabolisms, cytoskeleton-associated proteins, detoxification, homeostasis, cell communication, constitutive and allergen. This study provides new insights of the protein content in the Dufour's gland contributing to a more complete understanding of the biological functions of this gland in honeybees.

  13. Methods for species delimitation in bumblebees (Hymenoptera, Apidae, Bombus): towards an integrative approach

    Czech Academy of Sciences Publication Activity Database

    Lecocq, T.; Dellicour, S.; Michez, D.; Dehon, M.; Dewulf, A.; De Meulemeester, T.; Brasero, N.; Valterová, Irena; Rasplus, J. Y.; Rasmont, P.

    2015-01-01

    Roč. 44, č. 3 (2015), s. 281-297 ISSN 0300-3256 Institutional support: RVO:61388963 Keywords : bumblebee taxonomy * genetic analysis * pheromone composition Subject RIV: CC - Organic Chemistry Impact factor: 2.733, year: 2015

  14. Bee Diversity (Hymenoptera: Apoidea in a Tropical Rainforest Succession

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    Allan Smith-Pardo

    2007-01-01

    15.356 specimens were collected, belonging to four families and 287 species, representing 62% of all bee species found in Colombia. About 50% of all individuals sampled were stingless social bees (Apidae, Meliponini. Trigona (Trigona fulviventris was the most abundant species (~10% in the survey. Augochlora and Megachile were the most specious genera. The pasture and secondary forest showed high values of diversity and richness and were significantly higher than those of the mature forest and low shrubs. In all successional stages, except in the mature forest, the number of new species collected in each sample period approached zero and the species accumulation curves tended to stabilize as time and sampling area increased. The net was the most efficient method in all successional stages, except in the forest, where most bee species and individuals were collected with the Van Somer trap. However, a higher percentage (50% of rare species was collected with the Malaise trap. The number of new species collected in each sampled period and the species accumulation curves suggest that our survey was nearly sufficient to estimate the bee diversity in these early successional stages, but insufficient to study the mature forest apifauna. Due to the high efficiency of the Van Somer trap to attract bees in the forest, this trap should be used regularly in additional bee surveys in tropical rain forests. We also summarize the bee surveys in Colombia and highlight the importance of using other less common sampling methods to study bees from tropical ecosystems.

  15. Additions to the Knowledge of the Genus Phimenes (Hymenoptera: Vespidae: Eumeninae from Vietnam

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    Lien Thi Phuong

    2016-01-01

    Full Text Available The solitary wasp genus Phimenes Giordani Soika, 1992 (Hymenoptera: Vespidae: Eumeninae was reported to occur in Vietnam by van der Vecht (1959, represented by one taxon, Phimenes flavopictus continentalis (Zimmermann, which was synonymized under nominotypical Phimenes flavopictus (Blanchard, 1849 by Kumar (2013. A note on gender of this genus is made in the text. One more species, Phimenes indosinensis (van der Vecht, 1959 is recorded in this study from Dak Lak in the southern and Son La in the northwestern parts of Vietnam for the first time. Detailed descriptions of the female and male of the latter are provided with figures. A key to the two species from Vietnam is also provided.

  16. Two genera Foersteria Szépligeti, 1896 and Polydegmon Foerster, 1862 (Hymenoptera, Braconidae, Brachistinae) from China, with description of a new species.

    Science.gov (United States)

    Yan, Cheng-Jin; He, Jun-Hua; Chen, Xue-Xin

    2013-01-01

    The genera Foersteria Szépligeti, 1896 and Polydegmon Foerster, 1862 (Hymenoptera, Braconidae, Brachistinae) are recorded for the first time from China. A new species, Foersteria xinjiangensis Yan & Chen, sp. nov., is described and illustrated. A key to the Palaearctic species of Foersteria is given. In addition, Polydegmon sinuatus Foerster, 1862 is illustrated in detail for the first time.

  17. Extensive gene rearrangements in the mitochondrial genomes of two egg parasitoids, Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae).

    Science.gov (United States)

    Chen, Long; Chen, Peng-Yan; Xue, Xiao-Feng; Hua, Hai-Qing; Li, Yuan-Xi; Zhang, Fan; Wei, Shu-Jun

    2018-05-04

    Animal mitochondrial genomes usually exhibit conserved gene arrangement across major lineages, while those in the Hymenoptera are known to possess frequent rearrangements, as are those of several other orders of insects. Here, we sequenced two complete mitochondrial genomes of Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae). In total, 37 mitochondrial genes were identified in both species. The same gene arrangement pattern was found in the two species, with extensive gene rearrangement compared with the ancestral insect mitochondrial genome. Most tRNA genes and all protein-coding genes were encoded on the minority strand. In total, 15 tRNA genes and seven protein-coding genes were rearranged. The rearrangements of cox1 and nad2 as well as most tRNA genes were novel. Phylogenetic analysis based on nucleotide sequences of protein-coding genes and on gene arrangement patterns produced identical topologies that support the relationship of (Agaonidae + Pteromalidae) + Trichogrammatidae in Chalcidoidea. CREx analysis revealed eight rearrangement operations occurred from presumed ancestral gene order of Chalcidoidea to form the derived gene order of Trichogramma. Our study shows that gene rearrangement information in Chalcidoidea can potentially contribute to the phylogeny of Chalcidoidea when more mitochondrial genome sequences are available.

  18. The discovery of the genus Spasskia Belokobylskij, 1989 (Hymenoptera: Braconidae) in China, with description of a new species.

    Science.gov (United States)

    Yan, Cheng-jin; He, Jun-hua; Chen, Xue-xin

    2014-01-01

    The genus Spasskia Belokobylskij, 1989 (Hymenoptera: Braconidae: Helconinae) is reported for the first time from China. Two species, namely Spasskia brevicarinata Yan et Chen sp. n.and Spasskia indica Singh, Belokobylskij et Chauhan, 2005 are described and illustrated. A key to the species of this genus is updated to include the new species. This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.

  19. Ocorrência e recomendações para o manejo de Sirex noctilio Fabricius (Hymenoptera, Siricidae em plantios de Pinus patula (Pinaceae em Minas Gerais, Brasil Occurrence and management recommendations of Sirex noctilio Fabricius (Hymenoptera, Siricidae on Pinus patula (Pinaceae plantations in the state of Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Edson Tadeu Iede

    2007-12-01

    Full Text Available A vespa-da-madeira Sirex noctilio Fabricius, 1793 (Hymenoptera, Siricidae é a praga mais importante das florestas cultivadas com Pinus spp. no Brasil. Foi introduzida no Rio Grande do Sul em 1988 e sua dispersão ficou restrita aos estados do sul do país até 2004, quando foi detectada em São Paulo. Neste trabalho é relatada a ocorrência de S. noctilio em plantios de Pinus patula em Minas Gerais, em janeiro de 2005. São discutidas algumas medidas que podem ser adotadas para restringir a dispersão de S. noctilio na região Sudeste.The woodwasp Sirex noctilio Fabricius, 1793 (Hymenoptera, Siricidae is the most important pest on Pinus spp. in Brazil. It was introduced in the state of Rio Grande do Sul in 1988 and dispersed through the southern states. In 2004 it was detected in São Paulo state. In this work, it is reported the occurrence of S. noctilio in the state of Minas Gerais, in January 2005, on commercial areas of Pinus patula. It is discussed some measures that can be adopted to restrict its spread in the southeastern region.

  20. Proboscis conditioning experiments with honeybees, Apis mellifera caucasica, with butyric acid and DEET mixture as conditioned and unconditioned stimuli.

    Science.gov (United States)

    Abramson, Charles I; Giray, Tugrul; Mixson, T Andrew; Nolf, Sondra L; Wells, Harrington; Kence, Aykut; Kence, Meral

    2010-01-01

    Three experiments are described investigating whether olfactory repellents DEET and butyric acid can support the classical conditioning of proboscis extension in the honeybee, Apis mellifera caucasica (Hymenoptera: Apidae). In the first experiment DEET and butyric acid readily led to standard acquisition and extinction effects, which are comparable to the use of cinnamon as a conditioned stimulus. These results demonstrate that the odor of DEET or butyric acid is not intrinsically repellent to honey bees. In a second experiment, with DEET and butyric acid mixed with sucrose as an unconditioned stimulus, proboscis conditioning was not established. After several trials, few animals responded to the unconditioned stimulus. These results demonstrate that these chemicals are gustatory repellents when in direct contact. In the last experiment a conditioned suppression paradigm was used. Exposing animals to butyric acid or DEET when the proboscis was extended by direct sucrose stimulation or by learning revealed that retraction of the proboscis was similar to another novel odor, lavender, and in all cases greatest when the animal was not permitted to feed. These results again demonstrate that DEET or butyric acid are not olfactory repellents, and in addition, conditioned suppression is influenced by feeding state of the bee.

  1. Chemical Ecology of Stingless Bees.

    Science.gov (United States)

    Leonhardt, Sara Diana

    2017-04-01

    Stingless bees (Hymenoptera, Apidae: Meliponini) represent a highly diverse group of social bees confined to the world's tropics and subtropics. They show a striking diversity of structural and behavioral adaptations and are important pollinators of tropical plants. Despite their diversity and functional importance, their ecology, and especially chemical ecology, has received relatively little attention, particularly compared to their relative the honeybee, Apis mellifera. Here, I review various aspects of the chemical ecology of stingless bees, from communication over resource allocation to defense. I list examples in which functions of specific compounds (or compound groups) have been demonstrated by behavioral experiments, and show that many aspects (e.g., queen-worker interactions, host-parasite interactions, neuronal processing etc.) remain little studied. This review further reveals that the vast majority of studies on the chemical ecology of stingless bees have been conducted in the New World, whereas studies on Old World stingless bees are still comparatively rare. Given the diversity of species, behaviors and, apparently, chemical compounds used, I suggest that stingless bees provide an ideal subject for studying how functional context and the need for species specificity may interact to shape pheromone diversification in social insects.

  2. Captura de enxames de abelhas sem ferrão (Hymenoptera, Apidae, Meliponinae sem destruição de árvores Capturing stingless bee nests (Hymenoptera, Apidae, Meliponinae without destroying the trees

    Directory of Open Access Journals (Sweden)

    Alexandre Coletto-Silva

    2005-09-01

    Full Text Available No Brasil a criação racional de abelhas sem ferrão é denominada meliponicultura. As abelhas sem ferrão possuem diferentes comportamentos de nidificação, com ninhos internos (cavidades naturais ou não e externos. Um dos principais problemas apresentados na meliponicultura é a captura de uma colônia com o objetivo de iniciar um meliponário sem "destruir as árvores" ou mesmo as próprias colônias durante a captura. O presente trabalho apresenta um método alternativo para captura de colônias de abelhas sem ferrão, especialmente, do gênero Melipona Illiger, 1806, que são as espécies mais utilizadas para produção de mel e pólen, na região Amazônica. O método consiste em abrir uma janela na árvore, coletar o material e fechar a abertura utilizando a resina vegetal conhecida como breu.Meliponiculture is the name for stingless beekeeping in Brazil. Stingless bees have different nest behaviors showing external and internal nests (natural cavities or not. One of the main problems of meliponiculture is the capture of a colony in order to begin a meliponary without destroying trees or the colonies during the capture. A new alternative method for the capture of stingless bee colonies is presented for the genus Melipona Illiger, 1806, which are the species mostly used for producing honey and pollen. The method is to open the tree, collect the colony, and then close the tree with natural resins known as "breu".

  3. Pollen storages in nests of bees of the genera Partamona, Scaura and Trigona (Hymenoptera, Apidae Pólen estocado nos ninhos de abelhas dos gêneros Partamona, Scaura e Trigona (Hymenoptera, Apidae

    Directory of Open Access Journals (Sweden)

    André Rodrigo Rech

    2011-09-01

    Full Text Available Bees and angiosperms established a mutualistic relationship along the evolutionary time. The aim of this study is to contribute for the understanding of this relation analyzing pollen stored by stingless bees colonies distributed along the Rio Negro. Fourteen species of Meliponini from the genera Partamona, Scaura, and Trigona were studied with regard to the content of pollen pots. The pollen material was removed from the pollen pots, homogenized, and prepared according to the usual acetolysis technique. The overlap of the trophic niche and the grouping of species by similarity of niches was calculated. The identification revealed 78 pollen types belonging to 36 families, being 37 types attractive and 16 considered as promoters of a temporary specialization event. With the results, it was possible to indicate a list of important plants for meliponiculture in the Amazon.Abelhas e plantas estabeleceram ao longo do tempo evolutivo uma relação mutualística. Buscando contribuir para o entendimento dessa relação, foi analisado o pólen estocado por colônias de abelhas-sem-ferrão distribuídas ao longo do rio Negro. Foram estudados potes de pólen de 14 espécies de Meliponini dos gêneros Partamona, Scaura e Trigona. O material polínico foi retirado dos potes de pólen, homogeneizado e preparado segundo técnica usual de acetólise. Foram calculados a sobreposição de nicho trófico e o agrupamento das espécies pela similaridade de nichos. Foi identificado o total de 78 tipos polínicos, pertencentes a 36 famílias, sendo 37 destes, considerados atrativos, enquanto 16 foram promotores de eventos de especialização temporária. Com os resultados obtidos foi possível indicar uma lista de plantas de importância para a meliponicultura na Amazônia.

  4. Evidence for divergence in cuticular hydrocarbon sex pheromone between California and Mississippi (United States of America) populations of bark beetle parasitoid Roptrocerus xylophagorum (Hymenoptera: Pteromalidae).

    Science.gov (United States)

    Brian Sullivan; Nadir Erbilgin

    2014-01-01

    Roptrocerus xylophagorum (Ratzeburg) (Hymenoptera: Pteromalidae) is a common Holarctic parasitoid of the larvae and pupae of bark beetles (Coleoptera: Curculionidae: Scotytinae). In no-choice laboratory bioassays, we found that male wasps derived either from northern California or southwestern Mississippi, United States of America more frequently displayed sexual...

  5. Los Chalcidoidea (Hymenoptera asociados con agallas de Cinípidos (Hymenoptera, Cynipidae en la Comunidad de Madrid

    Directory of Open Access Journals (Sweden)

    Gómez, J. F.

    2006-05-01

    Full Text Available Information about the chalcid wasp parasitoid community (Hymenoptera, Chalcidoidea associated with galls of Cynipidae in Madrid (Spain is compiled and updated. Studied material includes more than 1000 published and unpublished records from samplings in 80 sites in the Madrid region carried out over twenty three years by the research team. A check-list of 121 species, 19 of them provisionally identified, from 6 families of Chalcidoidea is provided as follows: 26 Eurytomidae, 27 Torymidae, 9 Ormyridae, 33 Pteromalidae, 9 Eupelmidae and 17 Eulophidae. Ormyrus rufimanus Mayr, 1904 and Idiomacromerus semiaeneus (Szelenyi, 1957 are recorded for the first time in the Iberian Peninsula. For each family and genus of Chalcidoidea data are given on biology, diversity and distribution in Comunidad de Madrid. The composition of the chalcid wasp parasitoid community associated with gall wasps in Comunidad de Madrid is discussed and compared to the same community data from the Iberian Peninsula and the Western Palaearctic. Species from the Iberian community of chalcid parasitoids that are missing from Madrid region, exclusive species and potentially present species are also commented upon. Finally two appendices are presented as follows: a list of the Chalcidoidea species reared from 73 different galls made by 71 cynipid species from Madrid with associated parasitoid records, and a final appendix with full record data of all studied material, including information on the sampling localities with X, Y georeferenced coordinates, host galls and host plants.

    Se compila y actualiza la información existente sobre la comunidad parasitoide de Chalcidoidea, asociada a agallas de cinípidos (Hymenoptera, Cynipidae en la Comunidad de Madrid. Los datos corresponden a más de 1000 registros, tanto de datos publicados, como inéditos, correspondientes a colectas en 80 localidades de Madrid efectuadas por el equipo investigador a lo largo de 23 a

  6. Nearly complete mitogenome of hairy sawfly, Corynis lateralis (Brullé, 1832) (Hymenoptera: Cimbicidae): rearrangements in the IQM and ARNS1EF gene clusters.

    Science.gov (United States)

    Doğan, Özgül; Korkmaz, E Mahir

    2017-10-01

    The Cimbicidae is a small family of the primitive and relatively less diverse suborder Symphyta (Hymenoptera). Here, nearly complete mitochondrial genome (mitogenome) of hairy sawfly, Corynis lateralis (Hymenoptera: Cimbicidae) was sequenced using next generation sequencing and comparatively analysed with the mitogenome of Trichiosoma anthracinum. The sequenced length of C. lateralis mitogenome was 14,899 bp with an A+T content of 80.60%. All protein coding genes (PCGs) are initiated by ATN codons and all are terminated with TAR or T- stop codon. All tRNA genes preferred usual anticodons. Compared with the inferred insect ancestral mitogenome, two tRNA rearrangements were observed in the IQM and ARNS1EF gene clusters, representing a new event not previously reported in Symphyta. An illicit priming of replication and/or intra/inter-mitochondrial recombination and TDRL seem to be responsible mechanisms for the rearrangement events in these gene clusters. Phylogenetic analyses confirmed the position of Corynis within Cimbicidae and recovered a relationship of Tenthredinoidea + (Cephoidea + Orussoidea) in Symphyta.

  7. First report of interspecific facultative social parasitism in the paper wasp genus Mischocyttarus Saussure (Hymenoptera, Vespidae Primeiro registro de parasitismo social facultativo interespecífico em vespas do gênero Mischocyttarus Saussure (Hymenoptera, Vespidae

    Directory of Open Access Journals (Sweden)

    Thiago S. Montagna

    2012-06-01

    Full Text Available First report of interspecific facultative social parasitism in the paper wasp genus Mischocyttarus Saussure (Hymenoptera, Vespidae. Parasitism of colonies of the social wasp Mischocyttarus cerberus Ducke, 1918 by females of Mischocyttarus consimilis Zikán, 1949 was observed in a rural area of Dourados, state of Mato Grosso do Sul, Brazil. In all monitored cases, the invasion occurred in the pre-emergence colony stage, generally by a single female of M. consimilis. The period of establishment of the foreign female in the host colony was marked by antagonistic behaviors between the host female and the invasive. In general, the architecture of the parasitized nest was modified from the typical architecture of the host species nest.Primeiro registro de parasitismo social facultativo interespecífico em vespas do gênero Mischocyttarus Saussure (Hymenoptera, Vespidae. Parasitismo de colônias da vespa social Mischocyttarus cerberus Ducke, 1918 por fêmeas de Mischocyttarus consimilis Zikán, 1949 foram registrados em uma área rural no município de Dourados estado de Mato Grosso do Sul no Brasil. Em todos os casos monitorados a invasão ocorreu na fase colonial de pré-emergência, e em geral foi executado por uma única fêmea de M. consimilis. O período de estabelecimento da fêmea estrangeira na colônia hospedeira foi marcado por comportamentos antagônicos entre as fêmeas interespecíficas. Em geral, a arquitetura do ninho parasitado foi modificada em relação à arquitetura típica do ninho da espécie hospedeira.

  8. A new genus of oak gallwasp, Cyclocynips Melika, Tang & Sinclair (Hymenoptera: Cynipidae: Cynipini), with descriptions of two new species from Taiwan.

    Science.gov (United States)

    Melika, George; Tang, Chang-ti; Sinclair, Frazer; Yang, Man-miao; Lohse, Konrad; Hearn, Jack; Nicholls, James A; Stone, Graham N

    2013-01-01

    A new genus of cynipid oak gallwasp-Cyclocynips Melika, Tang, & Sinclair (Hymenoptera: Cynipidae: Cynipini), with two new species--C. uberis and C. tumorvirgae--reared from galls on oaks of the Quercus subgenus Cyclobalanopsis is described from Taiwan. Descriptions of asexual generation adults and their diagnostic characters are presented. The likelihood of yet undiscovered sexual generations and the evolution of host-plant associations in these species are discussed.

  9. Fauna de Campopleginae (Hymenoptera, Ichneumonidae) em hortas orgânicas em Araraquara e São Carlos, SP, Brasil

    OpenAIRE

    Sandonato, Diogo Loibel; Onody, Helena Carolina; Penteado-Dias, Angélica Maria

    2010-01-01

    Fauna de Campopleginae (Hymenoptera, Ichneumonidae) em hortas orgânicas em Araraquara e São Carlos, SP, Brasil. De março de 2006 a fevereiro 2007 foram realizadas doze amostragens utilizando armadilhas Malaise em cada uma das três áreas de cultivos orgânicos estudadas. Um total de 1773 espécimes foram identificados em dez gêneros de Campopleginae: Campoctonus Viereck, 1912, Campoletis Föster, 1869, Casinaria Holmgren, 1859, Charops Holmgren, 1859, Cryptophion Viereck, 1913, Diadegma Föster, 1...

  10. Ecology and Economics of Using Native Managed Bees for Almond Pollination.

    Science.gov (United States)

    Koh, Insu; Lonsdorf, Eric V; Artz, Derek R; Pitts-Singer, Theresa L; Ricketts, Taylor H

    2018-02-09

    Native managed bees can improve crop pollination, but a general framework for evaluating the associated economic costs and benefits has not been developed. We conducted a cost-benefit analysis to assess how managing blue orchard bees (Osmia lignaria Say [Hymenoptera: Megachildae]) alongside honey bees (Apis mellifera Linnaeus [Hymenoptera: Apidae]) can affect profits for almond growers in California. Specifically, we studied how adjusting three strategies can influence profits: (1) number of released O. lignaria bees, (2) density of artificial nest boxes, and (3) number of nest cavities (tubes) per box. We developed an ecological model for the effects of pollinator activity on almond yields, validated the model with published data, and then estimated changes in profits for different management strategies. Our model shows that almond yields increase with O. lignaria foraging density, even where honey bees are already in use. Our cost-benefit analysis shows that profit ranged from -US$1,800 to US$2,800/acre given different combinations of the three strategies. Adding nest boxes had the greatest effect; we predict an increase in profit between low and high nest box density strategies (2.5 and 10 boxes/acre). In fact, the number of released bees and the availability of nest tubes had relatively small effects in the high nest box density strategies. This suggests that growers could improve profits by simply adding more nest boxes with moderate number of tubes in each. Our approach can support grower decisions regarding integrated crop pollination and highlight the importance of a comprehensive ecological economic framework for assessing these decisions. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  11. Development of antennal sensilla of Tetragonisca angustula Latreille, 1811 (Hymenoptera: Meliponini during pupation

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    V. T. Dohanik

    Full Text Available Abstract The antennal sensilla are sensory organs formed by a group of neurons and accessory cells, which allow perception of environmental cues, which play a role as mechanoreceptors and chemoreceptors. This study describes the post-embryonic development of the antennal sensilla of the stingless Tetragonisca angustula (Hymenoptera: Meliponini workers. The development of the antennal sensilla begins in the transition stage of the pre-pupae to white-eyed pupae. The sensilla are completely developed at the black-eyed pupae stage, but they are covered by the old cuticle. The sensilla are exposed to the environment only in newly emerged workers of T. angustula, but it is possible that environmental stimuli can be recognized due to the pores in the old cuticle.

  12. Description of the male of Psyllaephagus euphyllurae (Masi (Hymenoptera, Encyrtidae, a parasitoid of the olive psylla, Euphyllura olivina (Costa (Hemiptera, Liviidae, with notes on its reproductive traits and hyperparasitoids

    Directory of Open Access Journals (Sweden)

    S.V. Triapitsyn

    2014-12-01

    Full Text Available A colony of the encyrtid wasp Psyllaephagus euphyllurae (Masi (Hymenoptera, Encyrtidae has been established in the quarantine laboratory at the University of California, Riverside, California, USA as part of a classical biological control program against its invasive host, the olive psylla, Euphyllura olivina (Costa (Hemiptera, Psylloidea, Liviidae, an important pest of olives in some parts of the world. The colony originators were reared from the same host found on abandoned, commercial olives in Catalonia, Spain; additional collections were made in Murcia. The parasitoid reproduces primarily by thelytoky; however, a few occasional males have been found in the field in Spain, but not in colonies reared under quarantine or laboratory conditions. Here, the female of P. euphyllurae is redescribed and its male is described and illustrated for the first time; the only previous mention of male P. euphyllurae was from Tunisia, reared from the same psyllid host but without any details on its morphology. A lectotype is designated for Encyrtus euphyllurae Masi. Information is given on the results of genetic matching between the two sexes of the parasitoid and also on the presence of the bacterial Wolbachia symbiont that apparently is affecting reproduction of this species, including its sex ratio in the field. Two species of hyperparasitoids have also emerged from the parasitized olive psylla nymphs from Catalonia: numerous specimens of Apocharips trapezoidea (Hartig (Hymenoptera, Figitidae and one specimen of a Pachyneuron sp. (Hymenoptera, Pteromalidae.

  13. Morphological and molecular characterization of common European species Adialytus (Hymenoptera: Braconidae: Aphidiinae) based on mtCOI barcoding gene and geometric morphometrics of the forewings

    Czech Academy of Sciences Publication Activity Database

    Stanković, S. S.; Petrović, A.; Milošević, M.I.; Starý, Petr; Kavallieratos, N. G.; Žikić, V.; Tomanović, Ž.

    2015-01-01

    Roč. 112, č. 1 (2015), s. 165-174 ISSN 1210-5759 Grant - others:Ministry of Education, Science and Technology Development of the Republic of Serbia(RS) III43001 Institutional support: RVO:60077344 Keywords : Hymenoptera * Braconidae * Adialytus Subject RIV: EG - Zoology Impact factor: 0.975, year: 2014 http://www.eje.cz/pdfs/eje/2015/01/21.pdf

  14. Partamona nigrilabris Pedro & Camargo, 2003, um novo sinônimo de P. nigrior (Cockerell, 1925 (Hymenoptera, Apidae Partamona nigrilabris Pedro & Camargo, 2003, a new synonym of P. nigrior (Cockerell, 1925 (Hymenoptera, Apidae

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    João M. F. Camargo

    2003-01-01

    Full Text Available P. nigrilabris was described based on specimens wrongly labelled as from "Salvador-BA, Brasil". It is considered as a new synonym: Partamona nigrior (Cockerell, 1925 = P. nigrilabris Pedro & Camargo, 2003, syn. nov.

  15. Study on the Hymenoptera parasitoid associated with Lepidoptera larvae in reforestation and agrosilvopastoral systems at Fazenda Canchim (Embrapa Pecuária Sudeste) São Carlos, SP, Brazil.

    Science.gov (United States)

    Pereira, A G; Silva, R B; Dias, M M; Penteado-Dias, A M

    2015-11-01

    The aim of this study was to characterize the local fauna of Hymenoptera parasitoids associated with Lepidoptera larvae in areas of reforestation and agrosilvopastoral systems at Fazenda Canchim (Embrapa Pecuária Sudeste, São Carlos, SP, Brazil). Lepidoptera larvae collected with entomological umbrella were kept in the laboratory until emergence of adults or their parasitoids. From those collected in the agrosilvopastoral system, emerged 267 specimens of hymenopteran parasitoids belonging to 16 genera: Braconidae, Agathidinae (Alabagrus), Braconinae (Bracon), Microgastrinae (Cotesia, Diolcogaster, Glyptapanteles, Pholetesor and Protapanteles), Orgilinae (Orgilus); Ichneumonidae, Campopleginae (Casinaria, Charops and Microcharops); Chalcididae, Chalcidinae (Brachymeria and Conura); Eulophidae, Entedoninae (Horismenus), Eulophinae (Elachertus and Euplectrus). From the Lepidoptera larvae collected in the reforestation, emerged 68 specimens of hymenopteran parasitoids, belonging to 8 genera: Chalcididae, Chalcidinae (Conura); Ichneumonidae, Pimplinae (Neotheronia), Campopleginae (Charops and Microcharops) and Braconidae, Microgastrinae (Apanteles, Diolcogaster, Distatrix, Glyptapanteles and Protapanteles). The results of this study suggest the occurrence of a wide variety of Hymenoptera parasitoids in the studied environments.

  16. Study on the Hymenoptera parasitoid associated with Lepidoptera larvae in reforestation and agrosilvopastoral systems at Fazenda Canchim (Embrapa Pecuária Sudeste São Carlos, SP, Brazil

    Directory of Open Access Journals (Sweden)

    A. G. Pereira

    Full Text Available Abstract The aim of this study was to characterize the local fauna of Hymenoptera parasitoids associated with Lepidoptera larvae in areas of reforestation and agrosilvopastoral systems at Fazenda Canchim (Embrapa Pecuária Sudeste, São Carlos, SP, Brazil. Lepidoptera larvae collected with entomological umbrella were kept in the laboratory until emergence of adults or their parasitoids. From those collected in the agrosilvopastoral system, emerged 267 specimens of hymenopteran parasitoids belonging to 16 genera: Braconidae, Agathidinae (Alabagrus, Braconinae (Bracon, Microgastrinae (Cotesia, Diolcogaster, Glyptapanteles, Pholetesor and Protapanteles, Orgilinae (Orgilus; Ichneumonidae, Campopleginae (Casinaria, Charops and Microcharops; Chalcididae, Chalcidinae (Brachymeria and Conura; Eulophidae, Entedoninae (Horismenus, Eulophinae (Elachertus and Euplectrus. From the Lepidoptera larvae collected in the reforestation, emerged 68 specimens of hymenopteran parasitoids, belonging to 8 genera: Chalcididae, Chalcidinae (Conura; Ichneumonidae, Pimplinae (Neotheronia, Campopleginae (Charops and Microcharops and Braconidae, Microgastrinae (Apanteles, Diolcogaster, Distatrix, Glyptapanteles and Protapanteles. The results of this study suggest the occurrence of a wide variety of Hymenoptera parasitoids in the studied environments.

  17. Eficiência de produtos termonebulígenos no controle de Atta laevigata (Hymenoptera: Formicidae em plantio de eucalipto Efficiency of products for thermonebulization on the control of Atta laevigata (Hymenoptera: Formicidae in eucalypus plantations

    Directory of Open Access Journals (Sweden)

    Ronald Zanetti

    2008-08-01

    Full Text Available Avaliou-se a eficiência de produtos termonebulígenos, a base de clorpirifós ou de extratos vegetais, comparativamente ao uso de isca formicida, a base de sulfluramida, no controle de Atta laevigata (F. Smith, 1858 (Hymenoptera: Formicidae. Foi avaliada a percentagem de paralisação das atividades de corte de folhas e de movimentação de formigas de A. laevigata aos três, 12, 36, 63 e 86 dias após a aplicação dos tratamentos. Na última avaliação, os formigueiros foram abertos para a verificação da eficiência de controle. Todos os produtos testados apresentaram alta percentagem de paralisação das atividades de corte e de movimentação das formigas aos três e 12 dias após a aplicação, respectivamente. Os produtos a base de clorpirifós e um a base de extratos vegetais apresentaram alta eficiência no controle de A. laevigata, sendo mais efetivos que a isca formicida testada.The efficiency of products formulated with chlorpyrifos or plants extracts in thermonebuzation was evaluated and compared to the use of the granulated bait formulated with sulfluramid in the control of Atta laevigata (F. Smith, 1858 (Hymenoptera: Formicidae. The cutting activity and the movement of ants on the colonies were evaluated at three, 12, 36, 63 and 86 days after the application of the treatments. The colonies of these ants were excavated in the last evaluation to obtain the efficiency of each product. All products stopped the cutting activity and movements of the individuals of A. laevigata three and 12 days after their application, respectively. Products formulated with chlorpyrifos and one with plant extracts were more efficient than granulated bait.

  18. Progeny Density and Nest Availability Affect Parasitism Risk and Reproduction in a Solitary Bee (Osmia lignaria) (Hymenoptera: Megachilidae).

    Science.gov (United States)

    Farzan, Shahla

    2018-02-08

    Gregarious nesting behavior occurs in a broad diversity of solitary bees and wasps. Despite the prevalence of aggregative nesting, the underlying drivers and fitness consequences of this behavior remain unclear. I investigated the effect of two key characteristics of nesting aggregations (cavity availability and progeny density) on reproduction and brood parasitism rates in the blue orchard bee (Osmia lignaria Say) (Hymenoptera: Megachilidae), a solitary species that nests gregariously and appears to be attracted to nesting conspecifics. To do so, I experimentally manipulated nest cavity availability in a region of northern Utah with naturally occurring populations of O. lignaria. Nest cavity availability had a negative effect on cuckoo bee (Stelis montana Cresson) (Hymenoptera: Megachilidae) parasitism rates, with lower parasitism rates occurring in nest blocks with more available cavities. For both S. montana and the cleptoparasitic blister beetle Tricrania stansburyi Haldeman (Coleoptera: Meloidae), brood parasitism rate was negatively correlated with log-transformed O. lignaria progeny density. Finally, cavity availability had a positive effect on male O. lignaria body weight, with the heaviest male progeny produced in nest blocks with the most cavities. These results suggest that cavity availability and progeny density can have substantial effects on brood parasitism risk and reproduction in this solitary bee species. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  19. Long-term monitoring of the introduced emerald ash borer (Coleoptera: Buprestidae) egg parasitoid, oobius agrili (Hymenoptera: Encyridae), in Michigan, USA and evaluation of a newly developed monitoring technique

    Science.gov (United States)

    Emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is a serious invasive pest of ash trees (Fraxinus spp.) in North America. The egg parasitoid Oobius agrili Zhang and Huang (Hymenoptera: Encyrtidae) was introduced as a biological control agent of this pest in Michiga...

  20. Effect of Parasitoid: Host Ratio and Parasitoid and Host Group Size on Fitness of Spathius galinae (Hymenoptera: Braconidae), a Parasitoid of Emerald Ash Borer (Coleoptera: Buprestidae): Implications for Mass-Rearing

    Science.gov (United States)

    Producing insect natural enemies in laboratories or insectaries for biological pest control is often expensive, and developing cost-effective rearing techniques is a goal of many biological control programs. Spathius galinae Belokobylskij and Strazenac (Hymenoptera: Braconidae), a recently described...

  1. Differences in the reproductive biology and diapause of two congeneric species of egg parasitoids (Hymenoptera:Encyrtidae) from northeast Asia: implications for biological control of the invasive emerald ash borer

    Science.gov (United States)

    Oobius primorskyensis Yao and Duan and Oobius agrili Zhang and Huang (Hymenoptera: Encyrtidae) constitute a cryptic species complex of egg parasitoids attacking the emerald ash borer Agrilus planipennis (Coleotpera: Buprestidae) in their native range of northeast Asia. While O. primorskyensis is c...

  2. Description of five species of Xanthopimpla Saussure 1892 (Hymenoptera: Ichneumonidae: Pimplinae) from Malaysia

    Science.gov (United States)

    Dass, Angeline David; Ghani, Idris Abd.

    2013-11-01

    Description of five species of Xanthopimpla Saussure, 1829 (Hymenoptera: Ichneumonidae: Pimplinae) from Malaysia was done using specimens deposited in Centre for Insects Systematics, Universiti Kebangsaan Malaysia (CIS, UKM). Type and non-type specimens were loaned from several repositories namely Zoological Museum of Amsterdam Netherlands (ZMAN), Swedish Museum of Natural History (NRM), British Natural History Museum London (BMNH) and Department of Agricultural Malaysia (DOA) for identification and comparison. The specimens were identified to the species level which gives rise to five species namely Xanthopimpla conica Cushman, 1925, Xanthopimpla despinosa leipephelis Townes & Chiu, 1970, Xanthopimpla flavolineata Cameron, 1907, Xanthopimpla punctata (Fabricius, 1781) and Xanthopimpla tricapus impressa Townes & Chiu, 1970. A dichotomous key and descriptions for five Xanthopimpla spesies were provided. Photos and illustrations of carina on propodeum were also included in this paper.

  3. A new mesoserphid wasp from the Middle Jurassic of northeastern China (Hymenoptera, Proctotrupoidea

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    Yan Zheng

    2017-12-01

    Full Text Available A new genus and species of Mesoserphidae (Hymenoptera, Juraserphus modicus gen. et sp. nov., is described based on a well-preserved fossil specimen from the Middle Jurassic Jiulongshan Formation of northeastern China. It is characterized by the following forewing features: the forking of Rs+M located approximately one-third of the distance between 1m-cu and 2r-rs, both 1cu-a and 2cu-a antefurcal; 1-M more than twice as long as 1m-cu and hind wing with cells r and rm closed. In addition, it has a short ovipositor, only extending slightly beyond the metasomal apex. Its new morphological characters broaden the diversity of Mesoserphidae in the Mesozoic and provide new insights into the evolution and relationships of Mesoserphidae.

  4. Revisions of the Afrotropical genera of Argidae and species of Pampsilota Konow, 1899 (Hymenoptera, Tenthredinoidea

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    Andrew D. Liston

    2017-01-01

    Full Text Available The Afrotropical fauna contains five genera of Argidae. These are keyed. New subjective synonyms, followed by the valid name in brackets, are Calarge Enslin, 1911 [Arge Schrank, 1802], Calarge africana Enslin, 1911 [Arge congrua Konow, 1907], Clyparge Pasteels, 1963 [Scobina Lepeletier & Serville, 1828], Clyparge terminalis Pasteels, 1963 [Scobina poecila (Klug, 1834], and Sterictophora [sic] afra Pasteels, 1963 [Sphacophilus afer comb. n., species inquirenda near S. monjarasi Smith & Morales-Reyes, 2015]. The type material of both C. terminalis and S. afra was probably collected in the New World, but labelled with the wrong locality “Kamerun”. An introduction of both species to Africa, not followed by long-term establishment, seems less likely. The removal of these taxa from the faunal list of the region is recommended. The nine known Afrotropical species of Pampsilota are revised, and an illustrated dichotomous identification key presented, with distribution maps for all species. Four species are here described as new to science: P. dahomeyanus Goergen, Koch & Liston, sp. n., P. nigeriae Liston & Koch, sp. n., P. tsavoensis Liston & Koch, sp. n., and P. zebra Liston & Koch, sp. n. Lectotypes are designated for Pampsilota afer Konow, 1899, and Cipdele africana Mocsáry, 1909. The immature stages and host plant of only one species are known: P. dahomeyanus on Lannea nigritana (Anacardiaceae. Its larval morphology strongly resembles that of European and North American species of Arge. We provisionally retain Pampsilota as a valid genus, although it could justifiably be treated as comprising merely a species group, or groups, within Arge.

  5. Revision of the Palaearctic Gasteruption assectator aggregate, with special reference to Sweden (Hymenoptera, Gasteruptiidae

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    Niklas Johansson

    2016-09-01

    Full Text Available The Palaearctic species of the Gasteruption assectator aggregate (Hymenoptera, Gasteruptiidae are revised and three species are recognised. Two species are re-instated: Gasteruption boreale (Thomson, 1883, stat. n. and G. nigritarse (Thomson, 1883, stat. n., and both are excluded from the synonymy with G. assectator (Linnaeus, 1758. The general distribution of both species is given for Europe and in detail for Sweden. A key to the valid Palaearctic species of the Gasteruption assectator aggregate is given; key characters and primary types are illustrated. Four new synonyms are listed: Foenus fumipennis Thomson, 1883, Trichofoenus breviterebrae Watanabe, 1934, and Gasteruption margotae Madl, 1987, are synonymized with Gasteruption boreale (Thomson, 1883 and Gasteruption brevicauda Kieffer, 1904, with G. undulatum (Abeille de Perrin, 1879.

  6. Lymphocyte-mediated regulation of platelet activation during desensitization in patients with hymenoptera venom hypersensitivity.

    Science.gov (United States)

    Ledru, E; Pestel, J; Tsicopoulos, A; Joseph, M; Wallaert, B; Tonnel, A B; Capron, A

    1988-01-01

    T cells from peripheral blood of hymenoptera sensitive patients were studied before and after venom desensitization. Before treatment, T cells showed a variable but higher proliferative response to allergen than T cells of treated patients or controls. While before desensitization, T cell products, specifically released after in vitro allergen stimulation, were able to amplify the IgE-dependent platelet activity, we showed that after treatment of the same patients, T cell products strongly reduced platelet activation. Considering the modifications in platelet activation previously observed in patients treated by specific immunotherapy, the present results suggest that, through a modification of T cell reactivity to allergen, T cell functions are modulated by desensitization, and emphasize the involvement of T cell products in the desensitization mechanisms. PMID:3263227

  7. Sting microsculpture in the digger wasp Bembix rostrata (Hymenoptera, Crabronidae

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    Natalia Matushkina

    2011-03-01

    Full Text Available The sting microsculpture of the digger wasp Bembix rostrata (Fabricius, 1781 (Hymenoptera, Crabronidae is studied with the scanning electron microscope (SEM for the first time. As in many other hymenopterans, the second valvifer of B. rostrata possesses two fields of styloconic sensilla (hair plates of proprioceptive function. The presence of two paired fields of campaniform sensilla on the second valvula and second valvifer is first shown in an apoid wasp. The first and the second valvulae bear scattered sensilla-like structures on the external surface, more numerous apically. The first valvula has two subapical barbs externally and a pair of valvilli on its inner surface, whereas the outer surface of the second valvula is smooth. The third valvula is sclerotized externally, consisting of proximal and distal parts, and bearing four sensilla morphotypes of mechanoreceptive and probably chemoreceptive functions. The inner surface of the valvulae and the membranous cuticle that is touching the sting have microstructures of different shapes directed distally. Functional aspects of characters studied are discussed.

  8. El subgénero Trigona S. Str. Jurine 1808 (Hymenoptera: Apidae: Meliponinae) en Colombia

    OpenAIRE

    Édgar Javier Hernández Martínez; Guiomar Nates Parra

    2004-01-01

    Para Colombia se registran 28 de los 29 taxones descritos para el subgénero Trigona s. str. T. (T.) albipennis Almeida, 1992; T. (T.) amalthea Olivier, 1789; T. (T.) hyalinata var. amazonensis Ducke, 1916; T. (T.) hyalinata var. branneri Cockerell, 1912; T. (T.) chanchamayoënsis Schwarz, 1948; T. (T.) cilipes Fabricius, 1804; T. (T.) corvina Cockerell, 1913; T. (T.) crassipes Fabricius, 1793; T. (T.) dallatorreana Friese, 1900; T. (T.) dimidiata var. venezuelana Schwarz, 1948; T. (T.) dimi...

  9. El subgénero Trigona S. Str. Jurine 1808 (Hymenoptera: Apidae: Meliponinae) en Colombia

    OpenAIRE

    Hernández Martínez Édgar Javier; Nates Parra Guiomar

    2004-01-01

    Para Colombia se registran 28 de los 29 taxones descritos para el subgénero Trigona s. str. T. (T.) albipennis Almeida, 1992; T. (T.) amalthea Olivier, 1789; T. (T.) hyalinata var. amazonensis
    Ducke, 1916; T. (T.) hyalinata var. branneri Cockerell, 1912; T. (T.) chanchamayoënsis Schwarz, 1948; T. (T.) cilipes Fabricius, 1804; T. (T.) corvina Cockerell, 1913; T. (T.) crassipes Fabricius, 1793; T. (T.) dallatorreana Friese, 1900; T. (T.) dimidiata var. venezuelana Schwarz, 1948; T. (...

  10. [Visitation of orchid by Melipona capixaba Moure & Camargo (Hymenoptera: Apidae), bee threatened with extinction].

    Science.gov (United States)

    Resende, Helder C; Barros, Fábio de; Campos, Lúcio A O; Fernandes-Salomão, Tânia M

    2008-01-01

    The stingless bee Melipona capixaba Moure & Camargo is a species restricted to the Atlantic forest in the Domingos Martins, Conceição do Castelo, Venda Nova do Imigrante and Afonso Cláudio County, in the Espírito Santo State, Brazil. Despite its cological importance as pollinator few studies have examined the ecology and biology of this bee. This note relates a case of the M. capixaba workers carrying pollinarium attached to the scuttellum. The pollinaria were identified as belonging to the orchid subtribe Maxillariinae species possibly of the genus Maxillaria sensu lato or Xylobium.

  11. Nesting habits of Centris (Hemisiella) dichrootricha (Hymenoptera: Apidae) in the Northern Cerrado of Brazil.

    Science.gov (United States)

    Carvalho, Gracy C A; Carreira, Léa M M; Rêgo, Márcia M C; Albuquerque, Patricia M C

    2016-09-01

    The Neotropical bee Centris (Hemisiella) dichrootricha is a solitary bee that nests in pre-existing cavities that occur in the rain forest. This study describes the nesting biology of C. dichrootricha and its preference for nesting in Cerrado and gallery forest habitats. The study was conducted from January 2012 and December 2013, in Mirador State Park in the municipality of Formosa da Serra Negra, Maranhão State, Brazil. For this, wooden trap-nests of 6, 8, 10, 12, 14 and 16 mm in diameter were used; a total of 300 trap-nests were placed in the gallery forest and Cerrado areas, respectively. Traps were monitored monthly and all completed nests were collected and replaced with empty ones. The nests were then taken to the laboratory to analyze bee development and emergence, nests characteristics and parasites presence. The species used 29 of the trap-nests, which had diameters of 8, 10, 12 and 14 mm. A total of 87 C. dichrootricha specimens emerged. The nests were parasitized by two bee species, Mesocheira bicolor (Apinae) and Coelioxys sp. (Megachilinae), and one fly species, Antrax sp. (Diptera). The highest nesting incidence of 72.4 % was observed in the gallery forest, whereas only 27.6 % in the Cerrado; this difference in habitat use was significant (χ² = 5.56; p pollen types that belonged to six families. Pollen of the family Malpighiaceae was most frequently used, with four species represented (Byrsonima crassifolia, B. rotunda, B. spicata and Heteropterys sp.). C. dichrootricha showed a preference for nesting in cavities of various diameters in gallery forest sites. The present study provides a novel description of the nesting habits and biology of C. dichrootricha in habitats of Central/Southern Maranhão. C. dichrootricha primarily used resources from the Cerrado, including soil to build their nests, pollen and floral oils; we concluded that gallery forest and Cerrado areas are intrinsically related to the maintenance of local populations of this species.

  12. Worker life tables, survivorship, and longevity in colonies of Bombus (Fervidobombus atratus (Hymenoptera: Apidae

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    Eunice Vieira da Silva-Matos

    2000-06-01

    Full Text Available Survivorship curves and longevity of workers were studied in two queenright and two queenless colonies of Bombus (Fervidobombus atratus. Survivorship curves for workers of all colonies were, in general, convex, indicating an increasing mortality rate with increasing age. The mean longevity for the workers from queenright colonies, 24.3 days and 17.6 days, was not significantly different from that in queenless colonies, 21.2 days and 20.2 days. In all colonies workers started foraging activities when aged 0-5 days, and the potential forager rates rose progressively with increasing age. Mortality rates within each age interval were significantly correlated with the foraging worker rates in all colonies. Only in two of the colonies (one queenright and one queenless longevity was significantly correlated with worker size. The duration of brood development period seems to be one of the most important factors influencing adult worker longevity in this bumble bee species.

  13. MORPHOLOGICAL CONSTRAINTS AND NECTAR ROBBING IN THREE ANDEAN BUMBLE BEE SPECIES (HYMENOPTERA, APIDAE, BOMBINI

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    RIVEROS ANDRE J.

    2006-06-01

    Full Text Available We report differences in foraging behavior of three Andean bumblebee species onflowers of Digitalis purpurea (Scrophulariaceae. Bombus atratus was a potentialpollinator while B. hortulanus and B. rubicundus collected nectar by robbing throughholes. We attribute behavioral differences to physical constraints. B. atratus has alonger glossa and a larger body size and is able to reach the nectaries, whereas B.hortulanus and B. rubicundus have shorter glossae and smaller bodies and probablymust rob nectar through holes at the base of flowers.

  14. Plastic nesting behavior of Centris (Centris) flavifrons (Hymenoptera: Apidae: Centridini) in an urban area

    OpenAIRE

    Martins , Celso; Peixoto , Marcella; Aguiar , Cândida

    2014-01-01

    International audience; Centris flavifrons is a ground-nesting solitary bee species that has an important role in crop and native plant species pollination in the Neotropical region. However, the nesting biology and ecology of this species are little understood. We studied the nesting biology of C. flavifrons by observing 287 nests in an urban garden. Females built nests only during the dry season from September to March and exhibit notable flexibility in the amount of time each nest is activ...

  15. The Synergistic Effects of Almond Protection Fungicides on Honey Bee (Hymenoptera: Apidae) Forager Survival.

    Science.gov (United States)

    Fisher, Adrian; Coleman, Chet; Hoffmann, Clint; Fritz, Brad; Rangel, Juliana

    2017-06-01

    The honey bee (Apis mellifera L.) contributes ∼$17 billion annually to the United States economy, primarily by pollinating major agricultural crops including almond, which is completely dependent on honey bee pollination for nut set. Almond growers face constant challenges to crop productivity owing to pests and pathogens, which are often controlled with a multitude of agrochemicals. For example, fungicides are often applied in combination with other products to control fungal pathogens during almond bloom. However, the effects of fungicides on honey bee health have been so far understudied. To assess the effects of some of the top fungicides used during the 2012 California almond bloom on honey bee forager mortality, we collected foragers from a local apiary and exposed them to fungicides (alone and in various combinations) at the label dose, or at doses ranging from 0.25 to 2 times the label dose rate. These fungicides were Iprodione 2SE Select, Pristine, and Quadris. We utilized a wind tunnel and atomizer set up with a wind speed of 2.9 m/s to simulate field-relevant exposure of honey bees to these agrochemicals during aerial application in almond fields. Groups of 40-50 foragers exposed to either untreated controls or fungicide-laden treatments were monitored daily over a 10-d period. Our results showed a significant decrease in forager survival resulting from exposure to simulated tank mixes of Iprodione 2SE Select, as well as synergistic detrimental effects of Iprodione 2SE Select in combination with Pristine and Quadris on forager survival. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  16. Evaluation of cage designs and feeding regimes for honey bee (Hymenoptera: Apidae) laboratory experiments.

    Science.gov (United States)

    Huang, Shao Kang; Csaki, Tamas; Doublet, Vincent; Dussaubat, Claudia; Evans, Jay D; Gajda, Anna M; Gregorc, Alex; Hamilton, Michele C; Kamler, Martin; Lecocq, Antoine; Muz, Mustafa N; Neumann, Peter; Ozkirim, Asli; Schiesser, Aygün; Sohr, Alex R; Tanner, Gina; Tozkar, Cansu Ozge; Williams, Geoffrey R; Wu, Lyman; Zheng, Huoqing; Chen, Yan Ping

    2014-02-01

    The aim of this study was to improve cage systems for maintaining adult honey bee (Apis mellifera L.) workers under in vitro laboratory conditions. To achieve this goal, we experimentally evaluated the impact of different cages, developed by scientists of the international research network COLOSS (Prevention of honey bee COlony LOSSes), on the physiology and survival of honey bees. We identified three cages that promoted good survival of honey bees. The bees from cages that exhibited greater survival had relatively lower titers of deformed wing virus, suggesting that deformed wing virus is a significant marker reflecting stress level and health status of the host. We also determined that a leak- and drip-proof feeder was an integral part of a cage system and a feeder modified from a 20-ml plastic syringe displayed the best result in providing steady food supply to bees. Finally, we also demonstrated that the addition of protein to the bees' diet could significantly increase the level ofvitellogenin gene expression and improve bees' survival. This international collaborative study represents a critical step toward improvement of cage designs and feeding regimes for honey bee laboratory experiments.

  17. Intraspecific variation of the cephalic labial gland secretions in Bombus terrestris (L.) (Hymenoptera: Apidae)

    Czech Academy of Sciences Publication Activity Database

    Coppée, A.; Terzo, M.; Valterová, Irena; Rasmont, P.

    2008-01-01

    Roč. 5, č. 12 (2008), s. 2654-2661 ISSN 1612-1872 Institutional research plan: CEZ:AV0Z40550506 Keywords : bumblebees * labial gland secretions * pheromones * male-marking pheromones * Bombus terrestris Subject RIV: CC - Organic Chemistry Impact factor: 1.659, year: 2008

  18. Meliponini neotropicais: o gênero Partamona Schwarz, 1939 (Hymenoptera, Apidae, Apinae - bionomia e biogeografia

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    Camargo João M. F.

    2003-01-01

    Full Text Available This work, dedicated to the study of nesting habits of the species of the Neotropical genus Partamona Schwarz, is a sequence to the taxonomic revision recently published elsewhere. A total of 214 nests and nest aggregations of 18 species [Partamona epiphytophila Pedro & Camargo, 2003; P. testacea (Klug, 1807; P. mourei Camargo, 1980; P. vicina Camargo, 1980; P. auripennis Pedro & Camargo, 2003; P. combinata Pedro & Camargo, 2003; P. chapadicola Pedro & Camargo, 2003; P. nhambiquara Pedro & Camargo, 2003; P. ferreirai Pedro & Camargo, 2003; P. pearsoni (Schwarz, 1938; P. gregaria Pedro & Camargo, 2003; P. batesi Pedro & Camargo, 2003; P. ailyae Camargo, 1980; P. cupira (Smith, 1863; P. mulata Moure in Camargo, 1980; P. seridoensis Pedro & Camargo, 2003; P. criptica Pedro & Camargo, 2003; P. helleri (Friese, 1900] were studied , including data about habitat, substrate, structural characteristics, construction materials and behavior. The descriptions of the nests are illustrated with 48 drawings. Partial data of the nests of P. bilineata (Say, 1837, P. xanthogastra Pedro & Camargo, 1997, P. orizabaensis (Strand, 1919, P. peckolti (Friese, 1901, P. aequatoriana Camargo, 1980, P. musarum (Cockerell, 1917 and P. rustica Pedro & Camargo, 2003 are also presented. Nests of P. grandipennis (Schwarz, 1951, P. yungarum Pedro & Camargo, 2003, P. subtilis Pedro & Camargo, 2003, P. vitae Pedro & Camargo, 2003, P. nigrior (Cockerell, 1925, P. sooretamae Pedro & Camargo, 2003 and P. littoralis Pedro & Camargo, 2003 are unknown. The species of Partamona build notable nest entrance structures, with special surfaces for incoming / exiting bees; some of them are extremely well-elaborated and ornamented, serving as flight orientation targets. All species endemic to western Ecuador to Mexico with known nesting habits (P. orizabaensis, P. peckolti, P. xanthogastra, P. bilineata, P. aequatoriana and P. musarum build their nests in several substrates, non-associated with termitaria, such as cavities and crevices in walls, among roots of epiphytes and in bases of palm leaves, in abandoned bird nests, under bridges, and in other protected places, except P. peckolti that occasionally occupies termite nests. In South America, on the eastern side of the Andes, only P. epiphytophila and P. helleri nest among roots of epiphytes and other substrates, non-associated with termitaria. All other species studied (P. batesi, P. gregaria, P. pearsoni, P. ferreirai, P. chapadicola, P. nhambiquara, P. vicina, P. mourei, P. auripennis, P. combinata, P. cupira, P. mulata, P. ailyae, P. seridoensis, P. criptica and P. rustica nest inside active termite nests, whether epigeous or arboreous. The only species that builds obligate subterranean nests, associated or not with termite or ant nests (Atta spp. is P. testacea. Nests of Partamona have one vestibular chamber (autapomorphic for the genus closely adjacent to the entrance, filled with a labyrinth of anastomosing pillars and connectives, made of earth and resins. One principal chamber exists for food and brood, but in some species one or more additional chambers are filled with food storage pots. In nests of P. vicina, there is one atrium or "false nest", between the vestibule and the brood chamber, which contains involucral sheaths, cells and empty pots. All structures of the nest are supported by permanent pillars made of earth and resins (another autapomorphy of the genus. The characters concerning nesting habits were coded and combined with morphological and biogeographic data, in order to hypothesize the evolutive scenario of the genus using cladistic methodology. The phylogenetic hypothesis presented is the following: (((((P. bilineata (P. grandipennis, P. xanthogastra (P. orizabaensis, P. peckolti (P. aequatoriana, P. musarum P. epiphytophila, P. yungarum, P. subtilis, P. vitae (((((P. testacea (P. mourei, P. vicina (P. nigrior (P. auripennis, P. combinata (P. ferreirai (P. pearsoni (P. gregaria (P. batesi (P. chapadicola, P. nhambiquara ((((P. ailyae, P. sooretamae P. cupira, P. mulata P. seridoensis P. criptica, P. rustica, P. littoralis P. helleri. One area cladogram is presented. Dates of some vicariance / cladogenesis events are suggested. For bilineata / epiphytophila group, which inhabits the Southwestern Amazonia and the Chocó-Mexican biogeographical components, the origin of ancestral species is attributed to the Middle Miocene, when the transgressions of the Maracaibo and Paranense seas isolated the tropical northwestern South America from the eastern continental land mass. The next cladogenic event in the history of the bilineata / epiphytophila group is attributed to the Plio-Pleistocene, when the Ecuadorian Andes reached more than 3000 m, and the ancestral species was fragmented in two populations, one occupying the western Andes (ancestral species of the bilineata subgroup and other the southwestern Amazon (ancestral species of the epiphytophila subgroup. Other aspects of the history of Partamona are also discussed.

  19. Eufriesea nigrescens y E. Pretiosa (Hymenoptera: Apidae: Euglossini: un caso de oportunismo o simbiosis?

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    González B. Victor Hugo

    2000-12-01

    Full Text Available Una colonia mixta se forma cuando un nido es ocupado por más de una especie (Michener 1974. En Bombus, este tipo de colonias son muy comunes de manera natural; la reina de una especie invade el nido de otra, mata a la reina hospedera y pone sus propias crías (Michener 1974. Este tipo de relaciones interespecíficas son raras en otros apidos y han sido registradas una sola vez entre dos especies de Meliponini: Melipona fuliginosa y M. panamica (= M. fasciata (Roubik 1981, Roubik, como pers., y dos especies de Euglossini: Eulaema cingulata y E. polychroma (Roubik 1990.

  20. New methods and media for the centrifugation of honey bee (Hymenoptera: Apidae) drone semen.

    Science.gov (United States)

    Wegener, Jakob; May, Tanja; Kamp, Günter; Bienefeld, Kaspar

    2014-02-01

    Centrifugation of Apis mellifera L. drone semen is a necessary step in the homogenization of semen pools for the enlargement of the effective breeding population, as well as in the collection of semen by the so-called washing technique. It is also of interest for the removal of cryoprotectants after cryopreservation. The adoption of methods involving semen centrifugation has been hampered by their damaging effect to sperm. Here, we tested four new diluents as well as three additives (catalase, hen egg yolk, and a protease inhibitor), using sperm motility and dual fluorescent staining as indicators of semen quality. Three of the new diluents significantly reduced motility losses after centrifugation, as compared with the literature standard. Values of motility and propidium iodide negativity obtained with two of these diluents were not different from those measured with untreated semen. The least damaging diluent, a citrate-HEPES buffer containing trehalose, was then tested in an insemination experiment with centrifuged semen. Most queens receiving this semen produced normal brood, and the number of sperm reaching the storage organ of the queen was not significantly different from that in queens receiving untreated semen. These results could improve the acceptance of techniques involving the centrifugation of drone semen. The diluent used in the insemination experiment could also serve as semen extender for applications not involving centrifugation.

  1. Uma espécie nova de Schwarzula da Amazônia (Hymenoptera, Apidae, Meliponini

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    Camargo João M. F.

    2002-01-01

    Full Text Available Schwarzula coccidophila sp. nov., a tiny Amazonian stingless bee, that attends scale insects (Cryptostigma Ferris, 1922, Coccidae in its nest, is described. It is distinguished from Schwarzula timida (Silvestri, 1902, the only other species of the genus, mainly by the malar area longer than diameter of 3rd flagellomere, and the denser plumose pilosity. Additional records of S. timida is presented.

  2. El subgénero Trigona S. Str. Jurine 1808 (Hymenoptera: Apidae: Meliponinae en Colombia

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    Édgar Javier Hernández Martínez

    2004-07-01

    basada en las características de la arquitectura externa de los nidos. Se discute la presencia de una especie nueva dentro del grupo Fulviventris. Se estableció una colección de referencia y una base de datos para el subgénero en el Laboratorio de Investigaciones en Abejas del departamento de Biología (LABUN de la Universidad Nacional de Colombia, Sede Bogotá.

  3. Temporal Activity Patterns of the Spider Wasp Pepsis montezuma Smith (Hymenoptera: Pompilidae) in a Disturbed Lower Montane Rainforest (Manizales, Colombia)

    OpenAIRE

    Restrepo-Giraldo, Carlos; Rodriguez, Juanita; Pitts, James P.

    2012-01-01

    We studied the temporal activity pattern of the spider wasp Pepsis montezuma Smith (Hymenoptera: Pompilidae) in a disturbed lower montane rainforest, which is located in the city of Manizales, Colombia, at an altitude of 2,150 m. Females of this species are diurnal with two peaks of activity: one in the morning and the other in the afternoon. During the morning, nectar foraging occurred at Baccharis latifolia. During the afternoon, females hunted for tarantulas of the genus Pamphobeteus (Aran...

  4. Las abejas (Hymenoptera: Apoidea del Parque Nacional Natural Gorgona, Pacífico colombiano

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    Marcela González-Córdoba

    2014-02-01

    Full Text Available En este estudio se presenta la diversidad y abundancia de las abejas del PNN Gorgona. En tres visitas en intervalos de tres meses, se muestrearon seis zonas de la isla con cubierta vegetal variable. Los muestreos fueron indirectos usando (i trampas McPhail (cebadas con cineol, silicato de metilo y extracto clavos; (ii trampas Van Someren Rydon (con cebo de pescado o pollo o fruta podridos, (iii trampas Malaise y (iv trampas de luz y directos a lo largo de transectos de longitud indefinida, por períodos de una hora en cada área y se repitieron cuatro veces. Un total de 585 especímenes fueron capturados, de ellos, 443 pertenecen a Apidae (14 géneros, 141 a Halictidae (seis géneros y uno a Megachilidae. Con este informe, el inventario local de Abejas se eleva a 43 especies y 28 géneros. Trigona fulviventris guianae Cockerell, 1910 fue la especie más abundante, Euglossa ignita Smith, 1874 la más atraída por los cebos aromáticos y Megalopta genalis Smith, 1853 dominó durante la noche. Apis mellifera Linnaeus, 1758 no se encontró en los muestreos. La importancia de la Isla Gorgona, como sitio de la diversidad para las especies de abejas es discutida.

  5. A cuckoo in wolves' clothing? Chemical mimicry in a specialized cuckoo wasp of the European beewolf (Hymenoptera, Chrysididae and Crabronidae

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    Herzner Gudrun

    2008-01-01

    Full Text Available Abstract Background Host-parasite interactions are among the most important biotic relationships. Host species should evolve mechanisms to detect their enemies and employ appropriate counterstrategies. Parasites, in turn, should evolve mechanisms to evade detection and thus maximize their success. Females of the European beewolf (Philanthus triangulum, Hymenoptera, Crabronidae hunt exclusively honeybee workers as food for their progeny. The brood cells containing the paralyzed bees are severely threatened by a highly specialized cuckoo wasp (Hedychrum rutilans, Hymenoptera, Chrysididae. Female cuckoo wasps enter beewolf nests to oviposit on paralyzed bees that are temporarily couched in the nest burrow. The cuckoo wasp larva kills the beewolf larva and feeds on it and the bees. Here, we investigated whether H. rutilans evades detection by its host. Since chemical senses are most important in the dark nest, we hypothesized that the cuckoo wasp might employ chemical camouflage. Results Field observations suggest that cuckoo wasps are attacked by beewolves in front of their nest, most probably after being recognized visually. In contrast, beewolves seem not to detect signs of the presence of these parasitoids neither when these had visited the nest nor when directly encountered in the dark nest burrow. In a recognition bioassay in observation cages, beewolf females responded significantly less frequently to filter paper discs treated with a cuticular extract from H. rutilans females, than to filter paper discs treated with an extract from another cuckoo wasp species (Chrysis viridula. The behavior to paper discs treated with a cuticular extract from H. rutilans females did not differ significantly from the behavior towards filter paper discs treated with the solvent only. We hypothesized that cuckoo wasps either mimic the chemistry of their beewolf host or their host's prey. We tested this hypothesis using GC-MS analyses of the cuticles of male and

  6. Características físico-químicas de amostras de mel de Melipona mandacaia Smith (Hymenoptera: Apidae Physico-chemical characteristics of honey samples of stingless bee Melipona mandacaia Smith (Hymenoptera: Apidae

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    Rogério Marcos de Oliveira Alves

    2005-12-01

    Full Text Available Análises de amostras de mel da abelha Melipona mandacaia provenientes do município de São Gabriel, região semi-árida do Estado da Bahia, foram realizadas com o objetivo de contribuir para o conhecimento das características físico-químicas desse produto. Os parâmetros analisados foram: Umidade (%; Hidroximetilfurfural (mg.kg-1; Açúcares Redutores (%; Sacarose (%; Viscosidade (mPa. s; Condutividade Elétrica (µS; pH; Acidez (meq.kg-1; Índice de Formol (mL.kg-1; e Cor. A maioria dos parâmetros físico-químicos apresentou valores médios adequados para o consumo humano, o que possibilita a exploração desse produto pelas comunidades rurais da região semi-árida da Bahia. Contudo, o teor de umidade elevado é um aspecto que requer uma maior atenção por parte do produtor, que deverá ter maiores cuidados com a higiene na manipulação do mel durante a coleta e no processo de armazenamento, evitando a sua contaminação por microrganismos que causam a depreciação do produto.Honey samples of the Melipona mandacaia stingless bee collected in the São Gabriel county, semi-arid region of the State of Bahia, Brazil, were analyzed with the objective of contributing for the knowledge of the characteristics physico-chemical of that product. The parameters analyzed were: Moisture (%; Hydroxymethylfurfural (mg.kg-1; Reducing Sugars (%; Sucrose (%; Viscosity (mPa.s; Electrical Conductivity (µS; pH; Acidity (meq.kg-1; Formol Index (mL.kg-1; and Color. Most of the physico-chemical parameters showed values adequated for the human consumption, facilitating the exploration of the product by rural communities of the semi-arid area of Bahia. However, the high moisture content is an aspect that deserves a greater attention by the part of producers, who should have concern with hygiene cares when manipulating the honey during the collection and the storage processes, avoiding its contamination with microorganisms that may cause depreciation of the product.

  7. Determinação das castas em Scaptotrigona postica (Latreille) (Hymenoptera, Apidae, Meliponini): diferenciação do ovário Caste determination in Scaptotrigona postica (Latreille) (Hymenoptera, Apidae, Meliponini): the ovarian differentiation

    OpenAIRE

    Thaís da Cruz Alves dos Santos; Carminda da Cruz-Landim

    2002-01-01

    Both castes of Scaptotrigolia postica (Latreille, 1804) possess four ovarioles in each ovary. Queen and workers have the same ovarian development during the larvallife, but in lhe late larval stage the queen ovary beco me larger. During pupation a higher rate of cell division is observed in queen ovarioles and a higher rale of cell death in workers. Newly emerged workers have short ovarioles with differenriatcd germarium and vitellarium while queens have very long ovarioles with only germariu...

  8. Determinação das castas em Scaptotrigona postica (Latreille (Hymenoptera, Apidae, Meliponini: diferenciação do ovário Caste determination in Scaptotrigona postica (Latreille (Hymenoptera, Apidae, Meliponini: the ovarian differentiation

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    Thaís da Cruz Alves dos Santos

    2002-09-01

    Full Text Available Both castes of Scaptotrigolia postica (Latreille, 1804 possess four ovarioles in each ovary. Queen and workers have the same ovarian development during the larvallife, but in lhe late larval stage the queen ovary beco me larger. During pupation a higher rate of cell division is observed in queen ovarioles and a higher rale of cell death in workers. Newly emerged workers have short ovarioles with differenriatcd germarium and vitellarium while queens have very long ovarioles with only germarium. Caste deterrnination in rhis species of bee is trophic, but lhe food does not differ in quality, only in quantiry. The food differences only beco me effecri ve by the end of larval stage when the queen larvae have lhe opportunity of eat more. In this way lhe ovary differentiation, between workers and queens. In this species, only occurs frorn lhe end of larval stage, mainly during pupation. Although the ovaries of workers are smaller, they are precocious in relation to queens, since nurse workers, 5 10 20 days, old may lay eggs. The eggs laid by the workers may be trophic or functional. These eggs may be distinguished by lhe aspect ofthe yolk. Older forager workers have degenerated ovaries.

  9. On the nesting biology of Pirhosigma Giordani Soika (Hymenoptera, Vespidae, Eumeninae, with special reference to the use of vegetable matter

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    Marcel G. Hermes

    2013-12-01

    Full Text Available On the nesting biology of Pirhosigma Giordani Soika (Hymenoptera, Vespidae, Eumeninae, with special reference to the use of vegetable matter. The use of vegetable matter in nest building is not widespread among the Eumeninae, and is reported for the first time for the two species of potter wasps Pirhosigma superficiale and P. limpidum. These wasps make mostly spherical mud nests over which they attach small pieces of unmasticated plant matter. Use of plant fragments in this group of wasps is interpreted as camouflage behavior.

  10. Anaphylactic Reactions After Discontinuation of Hymenoptera Venom Immunotherapy: A Clonal Mast Cell Disorder Should Be Suspected.

    Science.gov (United States)

    Bonadonna, Patrizia; Zanotti, Roberta; Pagani, Mauro; Bonifacio, Massimiliano; Scaffidi, Luigi; Olivieri, Elisa; Franchini, Maurizio; Reccardini, Federico; Costantino, Maria Teresa; Roncallo, Chiara; Mauro, Marina; Boni, Elisa; Rizzini, Fabio Lodi; Bilò, Maria Beatrice; Marcarelli, Anna Rosaria; Passalacqua, Giovanni

    2017-12-16

    Up to 75% of patients with severe anaphylactic reactions after Hymenoptera sting are at risk of further severe reactions if re-stung. Venom immunotherapy (VIT) is highly effective in protecting individuals with ascertained Hymenoptera venom allergy (HVA) and previous severe reactions. After a 3- to 5-year VIT course, most patients remain protected after VIT discontinuation. Otherwise, a lifelong treatment should be considered in high-risk patients (eg, in mastocytosis). Several case reports evidenced that patients with mastocytosis and HVA, although protected during VIT, can re-experience severe and sometimes fatal reactions after VIT discontinuation. To evaluate whether patients who lost protection after VIT discontinuation may suffer from clonal mast cell disorders. The survey describes the characteristics of patients who received a full course of VIT for previous severe reactions and who experienced another severe reaction at re-sting after VIT discontinuation. Those with a Red Española de Mastocitosis score of 2 or more or a serum basal tryptase level of more than 25 ng/mL underwent a hematological workup (bone marrow biopsy, KIT mutation, expression of aberrant CD25) and/or skin biopsy. Nineteen patients (mean age, 56.3 years; 89.5% males) were evaluated. All of them had received at least 4 years of VIT and were protected. After VIT discontinuation they were re-stung and developed, in all but 1 case, severe anaphylactic reactions (12 with loss of consciousness, in the absence of urticaria/angioedema). Eighteen patients (94.7%) had a clonal mast cell disorder, 8 of them with normal tryptase. Looking at this selected population, we suggest that mastocytosis should be considered in patients developing severe reactions at re-sting after VIT discontinuation and, as a speculation, patients with mastocytosis and HVA should be VIT-treated lifelong. Copyright © 2017. Published by Elsevier Inc.

  11. Dirhinus giffardii (Hymenoptera: Chalcididae, parasitoid affecting Black Soldier Fly production systems in West Africa

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    Emilie Devic

    2015-12-01

    Full Text Available Interest for insect farming is currently growing globally. Conditions in West Africa appear suitable for developing such farming systems that can benefit communities by improving livelihoods, food and feed security or sanitation. In Ghana and Mali, the Black Soldier Fly (Hermetia illucens Linnaeus, 1758 is being produced for waste recycling and animal feed. In a two stages process (egg and larvae production, egg production was hampered by a pupal parasitoid, Dirhinus giffardii Silvestri, 1913 (Hymenoptera: Chalcididae, which reduced future broodstock by almost 72%. This is the first time D. giffardii is reported as a parasitoid of H. illucens pupae and one of the first reports of parasitism in this commercially important fly species. The introduction of precautionary measures is highly recommended for the success of H. illucens production systems in West Africa.

  12. Notes on Neotropical Eumeninae, with the description of a new species of Pachodynerus de Saussure (Hymenoptera, Vespidae

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    Marcel Gustavo Hermes

    2013-06-01

    Full Text Available Notes on Neotropical Eumeninae, with the description of a new species of Pachodynerus de Saussure (Hymenoptera, Vespidae. Taxonomic information on Neotropical Eumeninae is provided. A new species, Pachodynerus fessatus sp. nov. is described from southeastern São Paulo, Brazil. Additional material of Pachodynerus sericeus (Fox was examined, representing the first further specimens after the original description and including the previously unknown male. The examination of new material of the genus Stenonartonia adds some new distribution records and shows some previously unrecorded individual variation for some species. The males of Stenonartonia guaraya Garcete-Barrett and Stenonartonia rejectoides Garcete-Barrett are described for the first time.

  13. A new Anagyrus (Hymenoptera: Encyrtidae) from Argentina, parasitoid of Hypogeococcus sp. (Hemiptera: Pseudococcidae) on Harrisia pomanensis (Cactaceae).

    Science.gov (United States)

    Triapitsyn, Serguei V; Aguirre, María B; Logarzo, Guillermo A

    2016-05-26

    A new species of Anagyrus Howard (Hymenoptera: Encyrtidae), A. lapachosus sp. n., is described from Salta Province of Argentina as a parasitoid of Hypogeococcus sp. (Hemiptera: Pseudococcidae) on Harrisia pomanensis cactus (Cactaceae). It is a candidate "new association" biological control agent for quarantine evaluation and possible following introduction to Puerto Rico (USA) against another Hypogeococcus sp., commonly called the Harrisia cactus mealybug and often misidentified as H. pungens Granara de Willink (according to our unpublished data the latter attacks only Amaranthaceae), which devastates or threatens the native cacti there and also in some other Caribbean islands (Triapitsyn, Aguirre et al. 2014; Carrera-Martínez et al. 2015).

  14. Antiviral Action of Hydromethanolic Extract of Geopropolis from Scaptotrigona postica against Antiherpes Simplex Virus (HSV-1).

    Science.gov (United States)

    Coelho, Guilherme Rabelo; Mendonça, Ronaldo Zucatelli; Vilar, Karina de Senna; Figueiredo, Cristina Adelaide; Badari, Juliana Cuoco; Taniwaki, Noemi; Namiyama, Gisleine; de Oliveira, Maria Isabel; Curti, Suely Pires; Evelyn Silva, Patricia; Negri, Giuseppina

    2015-01-01

    The studies on chemical composition and biological activity of propolis had focused mainly on species Apis mellifera L. (Hymenoptera: Apidae). There are few studies about the uncommon propolis collected by stingless bees of the Meliponini tribe known as geopropolis. The geopropolis from Scaptotrigona postica was collected in the region of Barra do Corda, Maranhão state, Brazil. The chemical analysis of hydromethanolic extract of this geopropolis (HMG) was carried out through HPLC-DAD-ESI-MS/MS and the main constituents found were pyrrolizidine alkaloids and C-glycosyl flavones. The presence of alkaloids in extracts of propolis is detected for the first time in this sample. The antiviral activity of HMG was evaluated through viral DNA quantification experiments and electron microscopy experiments. Quantification of viral DNA from herpes virus showed reduction of about 98% in all conditions and concentration tested of the HMG extract. The results obtained were corroborated by transmission electron microscopy, in which the images did not show particle or viral replication complex. The antiviral activity of C-glycosyl flavones was reported for a variety of viruses, being observed at different points in the viral replication. This work is the first report about the antiviral activity of geopropolis from Scaptotrigona postica, in vitro, against antiherpes simplex virus (HSV).

  15. Melanic variation underlies aposematic color variation in two hymenopteran mimicry systems.

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    Heather M Hines

    Full Text Available The stinging hymenopteran velvet ants (Mutillidae and bumble bees (Apidae: Bombus spp. have both undergone extensive diversification in aposematic color patterns, including yellow-red hues and contrasting dark-light body coloration, as a result of Müllerian mimicry. Understanding the genetic and developmental mechanisms underlying shifts in these mimetic colors requires characterization of their pigmentation. In this study, a combination of solubility, spectrophotometry, and melanin degradation analysis are applied to several color forms and species of these lineages to determine that orange-red colors in both lineages are comprised of primarily dopamine-derived pheomelanins. Until a few recent studies, pheomelanins were thought not to occur in insects. These results support their potential to occur across insects and particularly among the Hymenoptera. Shifts between black and orange-red colors, such as between mimetic color forms of bumble bee Bombus melanopygus, are inferred to involve modification of the ratios of dark eumelanins to red pheomelanins, thus implicating the melanin pathway in mimetic diversification. This discovery highlights the need to focus on how pheomelanins are synthesized in the insect melanin pathway and the potential for new pigments to be found even in some of our most well-known insect systems.

  16. Portable digital video surveillance system for monitoring flower-visiting bumblebees

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    Thorsdatter Orvedal Aase, Anne Lene

    2011-08-01

    Full Text Available In this study we used a portable event-triggered video surveillance system for monitoring flower-visiting bumblebees. The system consist of mini digital recorder (mini-DVR with a video motion detection (VMD sensor which detects changes in the image captured by the camera, the intruder triggers the recording immediately. The sensitivity and the detection area are adjustable, which may prevent unwanted recordings. To our best knowledge this is the first study using VMD sensor to monitor flower-visiting insects. Observation of flower-visiting insects has traditionally been monitored by direct observations, which is time demanding, or by continuous video monitoring, which demands a great effort in reviewing the material. A total of 98.5 monitoring hours were conducted. For the mini-DVR with VMD, a total of 35 min were spent reviewing the recordings to locate 75 pollinators, which means ca. 0.35 sec reviewing per monitoring hr. Most pollinators in the order Hymenoptera were identified to species or group level, some were only classified to family (Apidae or genus (Bombus. The use of the video monitoring system described in the present paper could result in a more efficient data sampling and reveal new knowledge to pollination ecology (e.g. species identification and pollinating behaviour.

  17. Antiviral Action of Hydromethanolic Extract of Geopropolis from Scaptotrigona postica against Antiherpes Simplex Virus (HSV-1

    Directory of Open Access Journals (Sweden)

    Guilherme Rabelo Coelho

    2015-01-01

    Full Text Available The studies on chemical composition and biological activity of propolis had focused mainly on species Apis mellifera L. (Hymenoptera: Apidae. There are few studies about the uncommon propolis collected by stingless bees of the Meliponini tribe known as geopropolis. The geopropolis from Scaptotrigona postica was collected in the region of Barra do Corda, Maranhão state, Brazil. The chemical analysis of hydromethanolic extract of this geopropolis (HMG was carried out through HPLC-DAD-ESI-MS/MS and the main constituents found were pyrrolizidine alkaloids and C-glycosyl flavones. The presence of alkaloids in extracts of propolis is detected for the first time in this sample. The antiviral activity of HMG was evaluated through viral DNA quantification experiments and electron microscopy experiments. Quantification of viral DNA from herpes virus showed reduction of about 98% in all conditions and concentration tested of the HMG extract. The results obtained were corroborated by transmission electron microscopy, in which the images did not show particle or viral replication complex. The antiviral activity of C-glycosyl flavones was reported for a variety of viruses, being observed at different points in the viral replication. This work is the first report about the antiviral activity of geopropolis from Scaptotrigona postica, in vitro, against antiherpes simplex virus (HSV.

  18. Stingless bees damage broccoli inflorescences when collecting fibers for nest building

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    Adriano Jorge Nunes dos Santos

    2012-01-01

    Full Text Available The stingless bee Trigona spinipes (Fabricius, 1793 (Hymenoptera: Apidae is an important pollinator for various crops, but constitutes an occasional pest of other plant species since it causes injury to leaves, stems, flowers and fruits while collecting nest materials. The aim of the present study was to determine the damage caused by T. spinipes to a broccoli (Brassica oleracea L. var. italica, Brassicaceae growing on an organic farm. A significant number of plants (72.5 % presented damaged inflorescences, while 39% of all of the inflorescences suffered some degree of injury. The activities of T. spinipes caused scarifications on the stems of the inflorescences, and these typically evolved to epidermal cicatrices up to 10 mm wide. In some cases, the lesions were sufficiently deep to cause partial destruction of the vascular tissues, and this lead to thinner (< 5 mm diameter floral stems that may collapse. To the best of our knowledge, this is the first report concerning the attack of broccoli plants by T. spinipes. The results obtained should serve to highlight the possibility that stingless bees could be responsible for direct and/or indirect damage to vegetable crops, and to stimulate the development of control strategies for these incidental pests.

  19. Reproductive performance of Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae) with previously refrigerated pupae of Bombyx mori L. (Lepidoptera: Bombycidae)

    OpenAIRE

    Pereira, FF.; Zanuncio, JC.; Serrão, JE.; Pastori, PL.; Ramalho, F.S.

    2009-01-01

    The mass rearing of parasitoids represents a fundamental stage for programmes of biological control. The progeny of the parasitoid Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae) were evaluated on previously refrigerated pupae of Bombyx mori L. (Lepidoptera: Bombycidae). Forty-eight to 72 hours-old pupae of B. mori were stored at 10 ºC for five, 10, 15 or 20 days and then exposed to parasitism by P. elaeisis females. This parasitoid showed shorter duration of the life cycl...

  20. First report of interspecific facultative social parasitism in the paper wasp genus Mischocyttarus Saussure (Hymenoptera, Vespidae

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    Thiago S. Montagna

    2012-06-01

    Full Text Available First report of interspecific facultative social parasitism in the paper wasp genus Mischocyttarus Saussure (Hymenoptera, Vespidae. Parasitism of colonies of the social wasp Mischocyttarus cerberus Ducke, 1918 by females of Mischocyttarus consimilis Zikán, 1949 was observed in a rural area of Dourados, state of Mato Grosso do Sul, Brazil. In all monitored cases, the invasion occurred in the pre-emergence colony stage, generally by a single female of M. consimilis. The period of establishment of the foreign female in the host colony was marked by antagonistic behaviors between the host female and the invasive. In general, the architecture of the parasitized nest was modified from the typical architecture of the host species nest.