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Sample records for leica rs500 leiat504

  1. Leica ADS40 Sensor for Coastal Multispectral Imaging

    Science.gov (United States)

    Craig, John C.

    2007-01-01

    The Leica ADS40 Sensor as it is used for coastal multispectral imaging is presented. The contents include: 1) Project Area Overview; 2) Leica ADS40 Sensor; 3) Focal Plate Arrangements; 4) Trichroid Filter; 5) Gradient Correction; 6) Image Acquisition; 7) Remote Sensing and ADS40; 8) Band comparisons of Satellite and Airborne Sensors; 9) Impervious Surface Extraction; and 10) Impervious Surface Details.

  2. System Software Testing of Laser Tracker Leica AT401

    Directory of Open Access Journals (Sweden)

    Filip Dvořáček

    2014-12-01

    Full Text Available The article introduces a group of instruments called laser trackers and specificallyfocuses on one of them - Leica AT401. At the Research Institute of Geodesy, To-pography and Cartography the instrument has been tested both in laboratory andoutdoor conditions. Several significant errors in the instrument’s system softwarehave been found, mostly due to the creation of user-programmed controlling appli-cation called ATControl. The errors are related to a selection, a computation andan evaluation procedure of the refractive index of air. Finally, notes of the newmeasurement modes of Leica AT40x are given and a range of distance measure-ment is discussed.

  3. Tecnologie RTK e Multiref GPS: SpiderNET di Leica

    Directory of Open Access Journals (Sweden)

    Redazione Redazione

    2005-07-01

    Full Text Available Il concetto fondamentale dell’approccio intrapreso da Leica è sostanzialmente quello di trasmettere tutti i dati rilevanti di correzione da un network di riferimento direttamente alrover in forma compatta rappresentando i dati di osservazione ed ambiguità livellata come differenziali di correzione per dati dispersivi e non-dispersivi; questa soluzione è ormai riconosciutacome Master-Auxiliary Concept (MAC e rappresenta la base per i messaggi RTCM 3.0 all’interno di network RTK.

  4. LABORATORY TESTING OF LEICA AT401 LASER TRACKER

    Directory of Open Access Journals (Sweden)

    Filip Dvořáček

    2016-04-01

    Full Text Available This paper describes laboratory tests on a Leica AT401laser tracker. As the newer Leica AT402 model also uses the same firmware package, most of the results should also be valid for this device. First, we present the instrument’s firmware errors and the software used for testing. The ASME B89.4.19-2006 standard for testing laser trackers is briefly presented. The warm-up effect of the instrument is inspected with respect to both angle measurement and distance measurement. The absolute distance meter (ADM is compared with a laboratory interferometer on a 30-meter long rail and also on a bench with automated movement of the carriage of the reflector. A time series of measurements for determining the additive constant is evaluated. A simple test of the stability of the distance measurement in field conditions is introduced. Most of the tests were carried out at the Research Institute of Geodesy, Topography and Cartography (RIGTC and at the Faculty of Civil Engineering (FCE of the Czech Technical University in Prague (CTU.

  5. Sample Preparation for TEM and FESEM: New Generation Coaters from Leica Microsystems

    Directory of Open Access Journals (Sweden)

    Niemova, S.V.

    2014-03-01

    Full Text Available The article informs about new desktop coaters for ap p lication of conductive layers in vacuum from Leica Mic ro systems. There are information about technical data and field of application of the coaters.

  6. Production data from a Leica ZBA31H+ shaped e-beam mask writer located at the Photronics facility, Manchester, England

    Science.gov (United States)

    Johnson, Stephen; Loughran, Dominic; Osborne, Peter; Sixt, Pierre; Doering, Hans-Joachim

    1999-06-01

    The ZBA31H+) is a variable shaped spot, vector scan e- beam lithography system operating at 20 keV. The specified performance is designed to produce reticles to 250 nanometer design rules, and beyond. In November 98 the acceptance results of a newly installed Leica ZBA31H+), at Photonic Manchester, were presented in a paper at the VDE/VDI 15th European Conference on Mask Technology. This paper is a continuation of that work and presents data from a capability study carried out, on 4000 angstrom EBR9 HS31 resist. Analysis of: mean to target, uniformity, X/Y bias, isolated vs. dense linewidths, linearity, and registration performance of the tool is presented, and the effects of re- iterative develop on process capability compared. Theoretically, a shaped beam system has advantages over raster scan in terms of write time and edge definition capabilities. In this paper, comparative write times against an Etec Mebes 4500 system are included. The ZBA31H+) has to write very small polygons in order to image non-axial or non-45 degree features. The resulting effect on image quality and write time is investigated. In order to improve the fidelity of small OPC structures, Leica have investigated alternative writing strategies, and their results to data are presented here.

  7. Acquisition of a Leica ScanStation II LIDAR unit

    Science.gov (United States)

    2008-04-01

    The funding will be used to purchase a LiDAR (Light Detection and Ranging) unit to generate external funding in many diverse areas. The investigators will initially seek funding from NSF, transportation agencies, and emergency management agencies for...

  8. Aerial Photography and Imagery, Ortho-Corrected, Leaf-on September 2004 0.5m resolution RGB orthoimagery that covers Connecticut's coastal communities. Data were collected by Earth Data, under contract to NOAA, using a Leica ADS40 sensor., Published in 2004, University of Connecticut.

    Data.gov (United States)

    NSGIC Education | GIS Inventory — Aerial Photography and Imagery, Ortho-Corrected dataset current as of 2004. Leaf-on September 2004 0.5m resolution RGB orthoimagery that covers Connecticut's coastal...

  9. Aerial Photography and Imagery, Ortho-Corrected, Leaf-on September 2004 0.5m resolution CIR orthoimagery that covers Connecticut's coastal communities. Data were collected by Earth Data, under contract to NOAA, using a Leica ADS40 sensor., Published in 2004, University of Connecticut.

    Data.gov (United States)

    NSGIC Education | GIS Inventory — Aerial Photography and Imagery, Ortho-Corrected dataset current as of 2004. Leaf-on September 2004 0.5m resolution CIR orthoimagery that covers Connecticut's coastal...

  10. Development of Osseointegrated Implants for Soldier Amputees Following Orthopaedic Extremity Trauma

    Science.gov (United States)

    2008-08-01

    Jung (Leica) 2050 Microtome (Leica Microsystems Inc., Bannockburn, IL) with Accu-Edge® Low Profile Blades (Sakura Finetek U.S.A., Inc., Torrance, CA...119-30. 18. Matsuzaki K. Why and how are peptide-lipid interactions utilized for self- defense? Magainins and tachyplesins as archetypes . Biochim

  11. 2004 Southwest Florida Water Management District Lidar: Sarasota District

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This metadata record describes the ortho & lidar mapping of Sarasota County, FL. The mapping consists of lidar data collected using a Leica ALS-40 Lidar Sensor,...

  12. 2008 Federal Emergency Management Agency (FEMA) New York Lidar: Erie County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Using Leica Geosystems ALS50 Laser Systems, 89 flight lines with a nominal point spacing = 1.4 meters (4.59 feet) were collected over Erie County, NY (approximately...

  13. Kui Che on reklaami(kunsti) ikooniks / Andres Laasik

    Index Scriptorium Estoniae

    Laasik, Andres, 1960-2016

    1999-01-01

    Eestis tehtud reklaamid on kahtlaselt sarnased välismaistega. Saksa ajakirjas "Spiegel" oleva ameerika fotoaparaadi Leica reklaam, millel on Che Guevara nägu, on sarnane internetikaubamaja "Osta" reklaamiga.

  14. Aerial Photography and Imagery, Ortho-Corrected - VOLUSIA 2006 Orthophotography

    Data.gov (United States)

    NSGIC Local Govt | GIS Inventory — 2006, 6 inch Pixel Color Orthophotography - - Panchromatic, red, green, blue and near infrared imagery was acquired using the Leica ADS40 multi-spectral scanner (see...

  15. 2010-2011 US Army Corps of Engineers (USACE) Joint Airborne Lidar Bathymetry Technical Center of Expertise (JALBTCX) Topobathy Lidar: Oregon and Washington

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These files contain topographic and bathymetric lidar data collected with the Leica ALS60 (topo) and SHOALS-1000T (bathy) systems along the coasts of Oregon and...

  16. Isolation and identification of Metarhizium anisopliae from Chilo ...

    African Journals Online (AJOL)

    *

    2012-04-12

    Apr 12, 2012 ... 1Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences (Key Laboratory of ... Keywords: Metarhizium anisopliae, isolation, identification, Chilo venosatus, culture medium, biological control. .... with a Leica microscope and average values were compared for all.

  17. 2007 ARRA Lidar: Lake County (IL)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Classified Point Cloud LAS files consist of 5 different classifications: Unclassified, Ground, Noise, Water and Ignored Ground. Merrick used the Leica ALS50...

  18. Immunotherapies for Targeting Ancient Retrovirus during Breast Cancer

    Science.gov (United States)

    2014-03-01

    cells group imaged for m-Kate expressed on A375-SM cells using a Leica whole body immunofluorescence microscope. The lungs of the tumor alone mice...analysis of IFC data. Bio-station time lapse imaging for analyzing T cells and tumor cell interaction in vitro over 24 hr period of time. Whole body ...immunoflourescence imaging -Leica Steromicroscope. COMPUTER SKILLS Windows, MS Word, Excel, Power Point, Adobe Photoshop , Adobe Acrobat, Adobe

  19. Refresher Course on Geomatic Applications for Sustainable Water ...

    Indian Academy of Sciences (India)

    Admin

    provided to and fro second class railway fare by the shortest route for attending the programme and each participant shall pay an amount of Rs. 500/- towards registration fee. Free boarding and lodging will be provided for outstation participants. This course is planned as a residential programme and stay in the University ...

  20. Fulltext PDF

    Indian Academy of Sciences (India)

    2. 3-year D.Math. (Hons.): Successful completion of 10+2 years of secondary education or equivalent with Mathematics and. English. Stipend Rs.500/- per month. 3. 2-year M.Stat.: 3-year Bachelor's degree with Statistics or Mathematics as a full subject, but those without Statistics must have outstanding mathematical ability.

  1. Focus stacking: Comparing commercial top-end set-ups with a semi-automatic low budget approach. A possible solution for mass digitization of type specimens

    Directory of Open Access Journals (Sweden)

    Jonathan Brecko

    2014-12-01

    Full Text Available In this manuscript we present a focus stacking system, composed of commercial photographic equipment. The system is inexpensive compared to high-end commercial focus stacking solutions. We tested this system and compared the results with several different software packages (CombineZP, Auto-Montage, Helicon Focus and Zerene Stacker. We tested our final stacked picture with a picture obtained from two high-end focus stacking solutions: a Leica MZ16A with DFC500 and a Leica Z6APO with DFC290. Zerene Stacker and Helicon Focus both provided satisfactory results. However, Zerene Stacker gives the user more possibilities in terms of control of the software, batch processing and retouching. The outcome of the test on high-end solutions demonstrates that our approach performs better in several ways. The resolution of the tested extended focus pictures is much higher than those from the Leica systems. The flash lighting inside the Ikea closet creates an evenly illuminated picture, without struggling with filters, diffusers, etc. The largest benefit is the price of the set-up which is approximately € 3,000, which is 8 and 10 times less than the LeicaZ6APO and LeicaMZ16A set-up respectively. Overall, this enables institutions to purchase multiple solutions or to start digitising the type collection on a large scale even with a small budget.

  2. Focus stacking: Comparing commercial top-end set-ups with a semi-automatic low budget approach. A possible solution for mass digitization of type specimens.

    Science.gov (United States)

    Brecko, Jonathan; Mathys, Aurore; Dekoninck, Wouter; Leponce, Maurice; VandenSpiegel, Didier; Semal, Patrick

    2014-01-01

    In this manuscript we present a focus stacking system, composed of commercial photographic equipment. The system is inexpensive compared to high-end commercial focus stacking solutions. We tested this system and compared the results with several different software packages (CombineZP, Auto-Montage, Helicon Focus and Zerene Stacker). We tested our final stacked picture with a picture obtained from two high-end focus stacking solutions: a Leica MZ16A with DFC500 and a Leica Z6APO with DFC290. Zerene Stacker and Helicon Focus both provided satisfactory results. However, Zerene Stacker gives the user more possibilities in terms of control of the software, batch processing and retouching. The outcome of the test on high-end solutions demonstrates that our approach performs better in several ways. The resolution of the tested extended focus pictures is much higher than those from the Leica systems. The flash lighting inside the Ikea closet creates an evenly illuminated picture, without struggling with filters, diffusers, etc. The largest benefit is the price of the set-up which is approximately € 3,000, which is 8 and 10 times less than the LeicaZ6APO and LeicaMZ16A set-up respectively. Overall, this enables institutions to purchase multiple solutions or to start digitising the type collection on a large scale even with a small budget.

  3. FIXED-BED COLUMN ADSORPTION OF METHYL BLUE USING ...

    African Journals Online (AJOL)

    userpc

    Adsorbent characterization. Scanning electron microscope (SEM, Leica. Stereoscan-440) and Fourier transform infrared spectroscopy (Cary630) Agilent Technologies in a range of 4000-400 cm-1, pHpzc (pH at point of zero charge) were used to investigate the physicochemical properties of the prepared adsorbent. Column ...

  4. Influence de la salinité sur la structure des branchies et l ...

    African Journals Online (AJOL)

    SARAH

    31 juil. 2014 ... appareil photographique numérique Leica DC 300 F associé au programme FW 4000. Les coupes ultrafines ont été contrastées à l'acétate d'uranyle et au citrate de ..... Osmoregulatory strategies in natural populations of the black-chinned tilapia Sarotherodon melanotheron exposed to extreme salinities in.

  5. Fulltext PDF

    Indian Academy of Sciences (India)

    preferably in food packaging applications because of its mag- nificent physical and mechanical properties ... LDPE also finds broad range applications because of its easy processability, light weight and chemical ..... with a diamond knife (M/s Leica, Germany) for subsequent. TEM observations. Images were captured by ...

  6. The morphological diversification of pollinia of some members of ...

    African Journals Online (AJOL)

    The morphological diversifications of pollinium of different genera of Asclepiadaceae were studied with the help of light microscope and phase contrast microscope (Leica-DM1000). The shape, size, position, orientation of pollinia, translator attachment, furrow position, etc are important criterion for the studies of pollinial ...

  7. Low voltage varistor ceramics based on SnO2

    Indian Academy of Sciences (India)

    WINTEC

    The microstructure of the sintered pellets was observed using a Leica Cambridge 440 microscope. The I–V characteristics were measured by using Keithley electrometer, 6517A. It also contained inbuilt 1 kV d.c. power supply so that there was no need of external power supply in the circuit. The current–voltage relation of a.

  8. Particularization of alpha contamination using CR-39 track detectors

    Indian Academy of Sciences (India)

    each was sandwiched to be dried in desiccant paper. To obtain the track diameter distribution and track density, the etched detec- tors were read and measured using an automatic digital image analysis system. (LEICA), consisting of a DMRE optical microscope with 600× magnification, a. Pramana – J. Phys., Vol. 69, No.

  9. Development and validation of an automated, microscopy-based method for enumeration of groups of intestinal bacteria

    NARCIS (Netherlands)

    Jansen, GJ; Wildeboer-Veloo, ACM; Tonk, RHJ; Franks, AH; Welling, G

    An automated microscopy-based method using fluorescently labelled 16S rRNA-targeted oligonucleotide probes directed against the predominant groups of intestinal bacteria was developed and validated. The method makes use of the Leica 600HR. image analysis system, a Kodak MegaPlus camera model 1.4 and

  10. Tuberculous Lymphadenitis in South-Eastern Nigeria; a 15 Years ...

    African Journals Online (AJOL)

    lives were saved between 2000 and 2013 through effective diagnosis and treatment.[1]. Tuberculous Lymphadenitis ... most common presentation while inguinal, axillary, mesenteric, mediastinal, and intramammary involvement have also .... The slides were examined using a binocular light microscope (Leica Microsystems ...

  11. Assessment of the biocompatibility of mineral trioxide aggregate ...

    African Journals Online (AJOL)

    2015-02-11

    Feb 11, 2015 ... periapical tissues and may penetrate through dentine.[4]. The biocompatibility of dental materials can be .... Analysis system (Leica Micros Imaging Solutions Ltd.,. Cambridge, UK). The intra‑class .... literature review – part II: Leakage and biocompatibility investigations. J Endod. 2010;36:190‑202. 6. Grecca ...

  12. Parameter Estimation And Hypothesis Testing In A Two Epoch Dam ...

    African Journals Online (AJOL)

    Also computed along with the least square solution and statistical testing were the minimum detectable Bias (MDB) and the Bias to Noise Ratio (BNR). All tests and adjustments were carried out using MOVE 3 software along with the LEICA SKI Pro 2.1. From the results of the tests, only observation to Rover station RF 8 ...

  13. hydrogeochemical assessment of groundwater in kaltungo and ...

    African Journals Online (AJOL)

    Admin

    of drinking water, but when contaminated, may lead to health problems (Aloa and Ige, 2003). The source of groundwater contamination could be natural through groundwater-rock interaction or through anthropogenic .... (Vacuum pump, Asbestos pad, Bukner flask and membrane funnel) and Leica Quebec Dark field colony.

  14. 2007 South Carolina LiDAR: Charleston (partial), Jasper, and Colleton Counties

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — LiDAR data collection was performed utilizing a Leica ALS-50 sensor, collecting multiple return x, y, and z data as well as intensity data. LiDAR data was processed...

  15. Download this PDF file

    African Journals Online (AJOL)

    acer

    tissue sections were placed in a water bath (Leica H1210, Germany) at 35°C to 37°C, and were then mounted on glass slides using a slide .... Up-and-Down procedures for testing of chemicals (OECD, 2008a), this finding indicates that the MECN is classified as category 5, ..... Fruit against human bacteria. Res. Pharma. Biol ...

  16. Electric field generated solitons, disclinations and vortical flows in ...

    Indian Academy of Sciences (India)

    observations were made in mercury light, in transmission or reflection mode, under a Leica polarizing microscope .... loop domains (figures 10c,d), depending on whether the approaching wave structures are in phase or ... [12], the absence of wave structure in thick films is explained as due to the diminished stabilizing effect ...

  17. Coendutermes tucum Fontes (Isoptera, Termitidae, Nasutitermitinae): description of the imago caste and additional notes.

    Science.gov (United States)

    Cuezzo, Carolina

    2016-12-09

    Coendutermes Fontes, 1985 is a monotypic South American termite genus. Coendutermes tucum Fontes, 1985, was described based on morphological characters from soldiers and workers collected in Mato Grosso, Brazil, and Jodensavanne, Suriname. Herein, I describe the imago caste of C. tucum for the first time with additional notes on soldiers, workers, and new distributional records. The studied material is deposited at the Museu de Zoologia da Universidade de São Paulo, São Paulo, Brazil (MZUSP). I use the terminology of Fontes (1987) to describe worker mandibles, and that of Noirot (2001) for the different parts of the digestive tube of workers. I measured the imagoes morphometric characters following Roonwal (1970): LH, length of head capsule (9); WH, width of head capsule without eyes (18); OF, occipito-fontanelle distance (23); DE, diameter of eye (48); LO, length of ocellus (55); WO, width of ocellus (56); EOD, eye-ocellus distance (57); LP, length of pronotum (65); WP, width of pronotum (68); LT, length of hind tibia (85). I took photographs of all castes with a stereomicroscope (Leica M205C) attached to a video camera (Leica DFC295) and images of gizzard and enteric valve under a microscope (Leica DM750B) attached to a video camera (Leica ICC50HD), then I combined the stacks of images with the software Leica LAS EZ 2.0 or Helicon Focus 5.2.11 X64. For the scanning electron micrographs (SEM), one soldier was dried to critical point while directly mounted on a stub with double face adhesive tape, then coated with gold and photographed with the SEM (Zeiss LEO 440 ®).

  18. A STUDY ON CASCADING IMPACT OF DEMONETIZATION ON LIVELIHOOD IN BELGAUM CITY OF KARNATAKA

    OpenAIRE

    SHIVASHANKAR K; Mr. BASAVARAJ TIGADI

    2017-01-01

    Demonetization has often been used as a tool to cut down hyper inflation. Which is essential to maintain country inflation in a moderate level in turn helps to have fastest growing economy in the world? Prime Minister of India Mr. Narendra Modi came out with his master stroke on corruption, counterfeit currency, terrorism and black money by announcing demonetization and ceasing Rs 500 and Rs. 1000 notes as a part of legal tender in India. Many of the scholars, politicians, business men and e...

  19. LHC survey laser tracker controls renovation

    International Nuclear Information System (INIS)

    Charrondiere, C.; Nybo, M.

    2012-01-01

    The LHC survey laser tracker control system is based on an industrial software package (Axyz) from Leica Geosystems (TM) that has an interface to Visual Basic (TM), which we used to automate the geometric measurements for the LHC magnets. With the new version of the Leica software, this Visual Basic (TM) interface is no longer available and we had to redesign the interface software to adapt to a PC-DMIS server that replaced the Axyz software. As this package is no longer supported, we have taken the decision to re-code the automation application in LabVIEW. This presentation describes the existing equipment, interface and application showing the reasons for our decisions to move to PC-DMIS and LabVIEW. A comparison between the new and the existing system is made. (authors)

  20. COMPARING MULTI-SOURCE PHOTOGRAMMETRIC DATA DURING THE EXAMINATION OF VERTICALITY IN A MONUMENTAL TOWER

    Directory of Open Access Journals (Sweden)

    J. Markiewicz

    2017-02-01

    Full Text Available This paper presents an analysis of source photogrammetric data in relation to the examination of verticality in a monumental tower. In the proposed data processing methodology, the geometric quality of the point clouds relating to the monumental tower of the castle in Iłżawas established by using terrestrial laser scanning (Z+F 5006h, Leica C10, terrestrial photographs and digital images sourced via unmanned aerial vehicles (UAV (Leica Aibot X6 Hexacopter. Tests were performed using the original software, developed by the authors, which allows for the automation of 3D point cloud processing. The software also facilitates the verification of the verticality of the tower and the assessment of the quality of utilized data.

  1. LHC Survey Laser Tracker Controls Renovation

    CERN Document Server

    Charrondière, C

    2011-01-01

    The LHC survey laser tracker control system is based on an industrial software package (Axyz) from Leica Geosystems™ that has an interface to Visual Basic™, which we used to automate the geometric measurements for the LHC magnets. With the new version of the Leica software, this Visual Basic™ interface is no longerb available and we had to redesign the interface software to adapt to a PC-DMIS server that replaced the Axyz software. As this package is no longer supported, we have taken the decision to recode the automation application in LabVIEW. This presentation describes the existing equipment, interface and application showing the reasons for our decisions to move to PC-DMIS and LabVIEW. A comparison between the new and legacy system is made

  2. Precision of GNSS instruments by static method comparing in real time

    Directory of Open Access Journals (Sweden)

    Slavomír Labant

    2009-09-01

    Full Text Available Tablet paper describes comparison of measuring accuracy two apparatus from the firm Leica. One of them recieve signals onlyfrom GPS satelites and another instrument is working with GPS and also with GLONASS satelites. Measuring is carry out by RTK staticmethod with 2 minutes observations. Measurement processing is separated to X, Y (position and h (heigh. Adjustment of directobservations is used as a adjusting method.

  3. Measurement Technology System

    Science.gov (United States)

    2015-05-29

    underwent two months of testing and integration in preparation for scanning unclassified Styrofoam replicas. The scanning of the critical areas of the...unclassified Styrofoam models of the propeller and the PC1A. Innovative’s Commack office received all of the LEICA scanning and electronic support...MTS Workstation and MTS Application software was shipped to Michigan for integration with the Robot Motion Programs. The Styrofoam replicas of the

  4. Microstructural Effects on the Spall Properties of ECAE-Processed AZ31B Magnesium Alloy

    Science.gov (United States)

    2016-10-01

    sectioned (parallel and perpendicular to the axis of the specimen) and metallographically prepared for the SEM examination. A Leica Microsystems optical ...mapped grains is shown in Fig. 2b. Use of this ECAE processing route re- sulted in the development of a strong< 0001 > fibre texture component (6–7...times random) parallel to the direction of the impact. The fibre texture is not ‘perfect’ as there is not normally distributed scatter about the ideal

  5. Qualitative and quantitative exfoliative cytology of normal oral mucosa in type 2 diabetic patients

    OpenAIRE

    Shareef, Ban Tawfeek; Ang, Kok Teong; Naik, Venkatesh Ramasamy

    2008-01-01

    Objective: The main purpose of this study is to emphasize the relevance of exfoliative cytology as an additional tool to aid in the diagnosis of diabetes mellitus. Materials and Methods: This is a comparative cross-sectional study. Oral smears were obtained from 10 diabetic patients and 10 healthy individuals. These smears were stained with Papanicolaou stain. The nuclear (NA) and cytoplasmic (CA) areas of 20 integral cells predominant in the buccal mucosa were measured using the Leica Qw...

  6. The measuring of real state of the residential complex Vlčince II in Žilina by using of TLS technology

    Directory of Open Access Journals (Sweden)

    Katarína Pukanská

    2011-12-01

    Full Text Available Construction of blocks of flats Vlčince II in Žilina, realized by the building company Doprastav a.s., consists from two blocksA and B. For measuring of real status construction was used terrestrial laser scanner Leica ScanStation. Processing of measured datawas applicated in software Cyclone Scan, Register and Cloudworx for Microstation. Through measured objects was created horizontalsections in more high levels. Founded deviations are presented in attached tables.

  7. Regulation of Programmed Necrosis and Bone Marrow Failure

    Science.gov (United States)

    2017-03-01

    process called programmed cell death removes cells, and bone marrow failure occurs when more cells are removed than can be replaced . There are two...hematopathologist. Whole slide images were obtained using Aperio Versa 200 (Leica Microsystems). We developed a pipeline for statistical analysis to evaluate the...cell death (PCD), apoptosis and recently recognized necroptosis, share molecular machinery, but diverge in outcome with important implications for the

  8. CMOS IMAGING SENSOR TECHNOLOGY FOR AERIAL MAPPING CAMERAS

    Directory of Open Access Journals (Sweden)

    K. Neumann

    2016-06-01

    Full Text Available In June 2015 Leica Geosystems launched the first large format aerial mapping camera using CMOS sensor technology, the Leica DMC III. This paper describes the motivation to change from CCD sensor technology to CMOS for the development of this new aerial mapping camera. In 2002 the DMC first generation was developed by Z/I Imaging. It was the first large format digital frame sensor designed for mapping applications. In 2009 Z/I Imaging designed the DMC II which was the first digital aerial mapping camera using a single ultra large CCD sensor to avoid stitching of smaller CCDs. The DMC III is now the third generation of large format frame sensor developed by Z/I Imaging and Leica Geosystems for the DMC camera family. It is an evolution of the DMC II using the same system design with one large monolithic PAN sensor and four multi spectral camera heads for R,G, B and NIR. For the first time a 391 Megapixel large CMOS sensor had been used as PAN chromatic sensor, which is an industry record. Along with CMOS technology goes a range of technical benefits. The dynamic range of the CMOS sensor is approx. twice the range of a comparable CCD sensor and the signal to noise ratio is significantly better than with CCDs. Finally results from the first DMC III customer installations and test flights will be presented and compared with other CCD based aerial sensors.

  9. Technical Training Seminar: Laser Trackers: the Local Positioning Technology (LPT)

    CERN Multimedia

    Davide Vitè

    2005-01-01

    Friday 20 May from 10:00 to 16:00, Training Centre (bldg. 593) Laser Trackers: the Local Positioning Technology (LPT) Simon Moser, Michael Lettau, Achim Lupus, Niklaus Suter, Leica GEOSYSTEMS AG, Switzerland Laser trackers are used at CERN for different applications within the LHC Project. Leica Geosystems AG have been developing during the last four years the revolutionary Local Positioning Technology (LPT). Laser trackers are increasingly used to ensure accuracy of large fabrications, and alignment in the final assembly process. Competing portable Coordinate Measuring Machines (CMM) with articulated arms require a frequent repositioning, known to lead to a loss of accuracy and efficiency. Leica Geosystems developed armless solutions, the T-Probe and T-Scan, for use with its laser trackers. The combination of the tracker technology with photogrammetry is the base of LPT, enabling real time measurements with free hand-held devices, such as the T-Probe and T-Scan. T-Probe and T-Scan overcome the proble...

  10. Insights on the molecular mechanism for the recalcitrance of biochars: interactive effects of carbon and silicon components.

    Science.gov (United States)

    Guo, Jianhua; Chen, Baoliang

    2014-08-19

    Few studies have investigated the effects of structural heterogeneity (particularly the interactions of silicon and carbon) on the mechanisms for the recalcitrance of biochar. In this study, the molecular mechanisms for the recalcitrance of biochars derived from rice straw at 300, 500, and 700 °C (named RS300, RS500, and RS700, respectively) were elucidated. Short-term (24 h) and long-term (240 h) oxidation kinetics experiments were conducted under different concentrations of H2O2 to distinguish the stable carbon pools in the biochars. We discovered that the stabilities of the biochars were influenced not only by their aromaticity but also through possible protection by silicon encapsulation, which is regulated by pyrolysis temperatures. The aromatic components and recalcitrance of the biochars increased with increasing pyrolysis temperatures. The morphologies of the carbon forms in all of the biochars were also greatly associated with those of silica. Silica-encapsulation protection only occurred for RS500, not for RS300 and RS700. In RS300, carbon and silica were both amorphous, and they were easily decomposed by H2O2. The separation of crystalline silica from condensed aromatic carbon in RS700 eliminated the protective role of silicon on carbon. The effect of the biochar particle size on the stability of the biochar was greatly influenced by C-Si interactions and by the oxidation intensities. A novel silicon-and-carbon-coupled framework model was proposed to guide biochar carbon sequestration.

  11. Morphological observation and characterization of thePseudoregma bambucicolawith the scanning electron microscope.

    Science.gov (United States)

    Nong, Xiang; Zeng, Xuemei; Yang, Yaojun; Liang, Zi; Tang, Mei; Liao, Lejuan; Luo, Chaobing

    2017-11-01

    Both leica microscopic camera system and scanning electron microscopy was used to observe and characterize the feet, back, abdomen, antennae and mouthparts of the Pseudoregma bambucicola from the bamboo, Bambusa multiplex . The possible functions of all the external morphological characteristics of the P. bambucicola were described and discussed in detail, which offers a basis for further enriching the biology, phylogeny and ecological niche of the P. bambucicola . Moreover, the morphological results should contribute to morphological identification and differentiation of the P. bambucicola from other aphids in the same family.

  12. San Miguel's dozers get it right the first time

    Energy Technology Data Exchange (ETDEWEB)

    Fiscow, S.

    2000-09-01

    The paper reports the latest machine guidance system developed for Leica Geosystem's new dozer 2000. The dozer 2000 system collects signals from the U.S. Global Positioning System (GPS) to determine the machine's position with centimeter-level accuracy in real time. Position data from a vehicle-mounted GPS receiver is fed to an AutoCAD-based engineering software package running on a rugged touch-screen computer in the operator's cab. The operation of the system, cost saving and the software components have been described. 4 photos.

  13. ALLTEM System User’s Manual, Munitions Management Projects, ALLTEM Multi-Axis Electromagnetic Induction System Demonstration and Validation, Version 1.0

    Science.gov (United States)

    2012-03-05

    parallel to. The sides are defined by their compass positions (SW, NW, NW, SE). If you don’t want the graph to be auto scaled on the X and Y axis, then...a neural network which classifies targets and generates a dig-list. Each of these menus are discussed in detail in the following sections...program is supplied by Leica, and uses the UTC time stamps on both the base and rover data sets to correlate the GPS values from the two units, at a

  14. HGF/c-MET Pathway in AIDS-Related Lymphoma

    Science.gov (United States)

    2016-09-01

    FBS, 10 mM HEPES ( pH 7.5), 100 U/mL of penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL amphotericin B. All cells were incubated at 37°C in 5...Transcriptional profiling of gastric epithelial cells infected with wild type or arginase-deficient Helicobacter pylori. BMC Microbiol. 2012; 12:175...DAPI; Sigma) in 180 mM Tris-HCl ( pH 7.5). Slides were washed once in 180 mM Tris-HCl for 15 minutes and prepared for visualization using a Leica

  15. HISTOMORFOMETRIA DO INTESTINO DELGADO DE RATOS SUBMETIDOS A DIFERENTES FONTES PROTÉICAS

    OpenAIRE

    M. CARUSO; A. DEMONTE

    2009-01-01

    Foi estudada a histomorfometria da porção inicial do intestino delgado de ratos, após a administração de concentrado protéico de soja e de caseína como únicas fontes protéicas. Foram mensurados o comprimento (CV), área (AV) das vilosidades e profundidade (PC) e área (AC) das criptas intestinais, através de microscópio óptico Leica Leitz DMRXE, acoplado com Câmera e Software Analisador de Imagens. Os resultados indicaram significativas diferenças entre os grupos avalia...

  16. Application of Geodetic Techniques for Antenna Positioning in a Ground Penetrating Radar Method

    Science.gov (United States)

    Mazurkiewicz, Ewelina; Ortyl, Łukasz; Karczewski, Jerzy

    2018-03-01

    The accuracy of determining the location of detectable subsurface objects is related to the accuracy of the position of georadar traces in a given profile, which in turn depends on the precise assessment of the distance covered by an antenna. During georadar measurements the distance covered by an antenna can be determined with a variety of methods. Recording traces at fixed time intervals is the simplest of them. A method which allows for more precise location of georadar traces is recording them at fixed distance intervals, which can be performed with the use of distance triggers (such as a measuring wheel or a hip chain). The search for methods eliminating these discrepancies can be based on the measurement of spatial coordinates of georadar traces conducted with the use of modern geodetic techniques for 3-D location. These techniques include above all a GNSS satellite system and electronic tachymeters. Application of the above mentioned methods increases the accuracy of space location of georadar traces. The article presents the results of georadar measurements performed with the use of geodetic techniques in the test area of Mydlniki in Krakow. A satellite receiver Leica system 1200 and a electronic tachymeter Leica 1102 TCRA were integrated with the georadar equipment. The accuracy of locating chosen subsurface structures was compared.

  17. A simple method for the extraction and identification of light density microplastics from soil.

    Science.gov (United States)

    Zhang, Shaoliang; Yang, Xiaomei; Gertsen, Hennie; Peters, Piet; Salánki, Tamás; Geissen, Violette

    2018-03-01

    This article introduces a simple and cost-saving method developed to extract, distinguish and quantify light density microplastics of polyethylene (PE) and polypropylene (PP) in soil. A floatation method using distilled water was used to extract the light density microplastics from soil samples. Microplastics and impurities were identified using a heating method (3-5s at 130°C). The number and size of particles were determined using a camera (Leica DFC 425) connected to a microscope (Leica wild M3C, Type S, simple light, 6.4×). Quantification of the microplastics was conducted using a developed model. Results showed that the floatation method was effective in extracting microplastics from soils, with recovery rates of approximately 90%. After being exposed to heat, the microplastics in the soil samples melted and were transformed into circular transparent particles while other impurities, such as organic matter and silicates were not changed by the heat. Regression analysis of microplastics weight and particle volume (a calculation based on image J software analysis) after heating showed the best fit (y=1.14x+0.46, R 2 =99%, p80%. Results from field samples collected from North-western China prove that our method of repetitive floatation and heating can be used to extract, distinguish and quantify light density polyethylene microplastics in soils. Microplastics mass can be evaluated using the empirical model. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. Combined techniques for network measurements at accelerator facilities

    International Nuclear Information System (INIS)

    Pschorn, I.

    1999-01-01

    Usually network measurements at GSi (Gesellschaft fur Schwerionen forschung) are carried out by employing the Leica tachymeter TC2002K etc. Due to time constraints and the fact that GSi possesses only one of these selected, high precision total-stations, it was suddenly necessary to think about employing a Laser tracker as the major instrument for a reference network measurement. The idea was to compare the different instruments and to proof if it is possible at all to carry out a precise network measurement using a laser tracker. In the end the SMX Tracker4500 combined with Leica NA3000 for network measurements at GSi, Darmstadt and at BESSY Il, Berlin (both located in Germany) was applied. A few results are shown in the following chapters. A new technology in 3D metrology came up. Some ideas of applying these new tools in the field of accelerator measurements are given. Finally aspects of calibration and checking the performance of the employed high precision instrument are pointed out in this paper. (author)

  19. Estimation of vegetation water content at leaf and canopy level using dual-wavelength commercial terrestrial laser scanners.

    Science.gov (United States)

    Elsherif, Ahmed; Gaulton, Rachel; Mills, Jon

    2018-04-06

    Vegetation water content, quantified as the leaf equivalent water thickness (EWT), can serve as an indicator of vegetation stress. The intensity data recorded by terrestrial laser scanning (TLS) instruments, operating at shortwave infrared wavelengths, can be used to estimate the three-dimensional distribution of EWT, after a full and rigorous calibration for the range and incidence angle effects. However, TLS instruments do not record the incidence angles automatically, making calibration challenging. In this study, intensity data from two commercially available TLS instruments (Leica P40, 1550 nm shortwave infrared wavelength, and Leica P20, 808 nm near-infrared wavelength) were combined in a normalized difference index (NDI). The NDI was found to minimize the incidence angle effects with no need for further calibration. A dry-down experiment was conducted using deciduous and conifer canopies. The NDI was found to be highly correlated to EWT at leaf level ( R 2 of 0.91 and 0.74) and at canopy level ( R 2 of 0.89 and 0.74) for the deciduous and conifer canopies, respectively. Three-dimensional distributions of EWT at canopy level were generated, which revealed some vertical heterogeneity.

  20. Prvi optički teodoliti : First optical theodolites

    Directory of Open Access Journals (Sweden)

    Nedim Tuno

    2011-12-01

    Full Text Available U radu je dat pregled prvih optičkih teodolita, koji su označili veliku prekretnicu u razvoju i primjeni geodetskih instrumenata. Njihova pojava veže se za ime čuvenog konstruktora Heinricha Wilda i imala je nemjerljivo pozitivan uticaj na cjelokupnu industriju geodetskih instrumenata, koji se osjeća čak i danas. Svrha članka nije komercijalna u smislu reklamiranja pojedinih tehničkih rješenja iz tvornice Wild (Leica, već pokušaj da se prikaže mjerna tehnologija koja je dominirala u prethodnom stoljeću, kroz prizmu legendarnog teodolita T2. : The article gives an overview of the first optical theodolites, which marked a major milestone in the development and use of surveying instruments. Their appearance is connected to the name of the famous constructor Heinrich Wild and had a immeasurably positive impact on the entire industry of surveying instruments, that is still felt today. The purpose of the article is not commercial in terms of advertising of certain technical solutions of the manufacturer Wild (Leica, but an attempt to show the measurement technology that has dominated the previous century, through the lens of legendary T2 theodolite.

  1. IMMUNOHISTOCHEMICAL ANALYSIS OF CASPASE-3 ACTIVITY IN LIVER BIOPSIES OF PATIENTS WITH MONO AND MIXED INFECTIONS

    Directory of Open Access Journals (Sweden)

    I. I. Tokin

    2015-01-01

    Full Text Available Objective: to study the activity of proapoptotic signal protein caspase-3 for determination of peculiarities of apoptosis regulation under liver chronic diseases.Subjects and methods. The immunohistochemical analysis of caspase-3 activity in 5 liver biopsies of the patients with mono infection of chronic hepatitis B and 5 liver biopsies of the patients with mixed infection of tuberculosis, chronic hepatitis C and human immunodeficiency virus was fulfilled. Morphological and morphometric analysis of serial microphotographs was performed using an image analysis system (microscope Leica DM 2500, digital camera Leica DFC320 R2 and a computer.Results. The activity of caspase-3 as dark brown granularity was revealed in all tis-sue components of liver (hepatocytes, epithelium of bile ducts, endotheliocytes, Kupffer cells of sinusoids, in compositions of lymphohistiocyte infiltrations. The maximal activity was discovered in hepatocytes nuclei. The expression of caspase-3 was significantly higher in liver biopsies of the patients with mixed infection. It is typical that the immunoreactive hepatocytes had not any morphological marks of apoptosis.Conclusion. The caspase-3 expression of proapoptotic signal protein caspase-3 may serve as an early marker of liver damage including the possibilities of apoptosis development.

  2. IMMUNOHISTOCHEMICAL ANALYSIS OF CASPASE-3 ACTIVITY IN LIVER BIOPSIES OF PATIENTS WITH MONO AND MIXED INFECTIONS

    Directory of Open Access Journals (Sweden)

    I. I. Tokin

    2014-01-01

    Full Text Available Objective: to study the activity of proapoptotic signal protein caspase-3 for determination of peculiarities of apoptosis regulation under liver chronic diseases.Subjects and methods. The immunohistochemical analysis of caspase-3 activity in 5 liver biopsies of the patients with mono infection of chronic hepatitis B and 5 liver biopsies of the patients with mixed infection of tuberculosis, chronic hepatitis C and human immunodeficiency virus was fulfilled. Morphological and morphometric analysis of serial microphotographs was performed using an image analysis system (microscope Leica DM 2500, digital camera Leica DFC320 R2 and a computer.Results. The activity of caspase-3 as dark brown granularity was revealed in all tis-sue components of liver (hepatocytes, epithelium of bile ducts, endotheliocytes, Kupffer cells of sinusoids, in compositions of lymphohistiocyte infiltrations. The maximal activity was discovered in hepatocytes nuclei. The expression of caspase-3 was significantly higher in liver biopsies of the patients with mixed infection. It is typical that the immunoreactive hepatocytes had not any morphological marks of apoptosis.Conclusion. The caspase-3 expression of proapoptotic signal protein caspase-3 may serve as an early marker of liver damage including the possibilities of apoptosis development.

  3. Comparing microleakage and the layering methods of silorane-based resin composite in wide Class II MOD cavities.

    Science.gov (United States)

    Bagis, Yildirim Hakan; Baltacioglu, Ismail Hakki; Kahyaogullari, Savas

    2009-01-01

    This in vitro study compared the effects of different layering techniques and different monomer-based composites on microleakage. Thirty-two freshly extracted caries-free human third molars were used. A wide mesio-occlusal-distal (MOD) cavity was prepared in each third molar, with the occlusal dimension being approximately two-thirds of the intercuspal dimension, and the cervical limit of one proximal box was stopped 1 mm below and the other 1 mm above the cementoenamel junction. The cavities were filled with a methacrylate-based nanohybrid composite (Grandio, VoCo) and a silorane-based microhybrid composite (Filtek Silorane, 3M ESPE). All samples were subjected to 1000 thermal cycles of 5 degrees C/55 degrees C in water with a 30-second dwell time and, after the procedure, the teeth were immersed in a 0.5% basic fuchsin dye at 23 degrees C for 24 hours. Sectioned samples were examined under a stereomicroscope (Leica MZ12, Leica Microsystems), and microleakage scores were statistically analyzed using the Kruskal-Wallis and the Mann-Whitney U tests. No microleakage was observed for silorane-based resin composite restorations. The nanohybrid-based resin composite restorations showed different levels of leakage. Statistical analysis of microleakage scores showed differences only at the enamel margins for nanohybrid material for both layering techniques (p < 0.05). The nanohybrid-based resin composite restorations showed better results with the vertical layering technique for enamel margins.

  4. La documentación geométrica de elementos patrimoniales con láser escáner terrestre. La experiencia del Lapa en Galicia

    Directory of Open Access Journals (Sweden)

    Mañana Borrazás, Patricia

    2009-12-01

    Full Text Available In this text we show some geometric documentation of various elements of Cultural Heritage. We use a Terrestrial Laser Scanner, a Leica HDS3000, a survey instrument that allows a high density register with pinpoint accuracy. The basic aim of this article is to evaluate the documentation posibilities of this kind of Terrestrial Laser Scanner and the different problems that emerged. As an example, we present the work processing of the topographic survey of a hillfort, the record in full detail of an archaeological excavation, the survey of a building and a two site of Rock Art.

    En este texto se presentan una serie de trabajos de documentación geométrica de diversos elementos patrimoniales realizados con láser escáner 3D. En ellos hemos empleado el modelo de Leica HDS3000, un instrumento topográfico que permite una captura de datos densa y con una resolución milimétrica. El objetivo principal de este documento es evaluar las posibilidades de registro de este escáner en distintos tipos de sitios así como los problemas que se generaron. Como ejemplos, presentamos el proceso de trabajo para obtener la topografía de un castro, el registro de detalle de una excavación, el levantamiento de un edificio y de unos petroglifos.

  5. ANALYSIS OF TERRESTRIAL LASER SCANNING AND PHOTOGRAMMETRY DATA FOR DOCUMENTATION OF HISTORICAL ARTIFACTS

    Directory of Open Access Journals (Sweden)

    R. A. Kuçak

    2016-10-01

    Full Text Available Historical artifacts living from the past until today exposed to many destructions non-naturally or naturally. For this reason, The protection and documentation studies of Cultural Heritage to inform the next generations are accelerating day by day in the whole world. The preservation of historical artifacts using advanced 3D measurement technologies becomes an efficient tool for mapping solutions. There are many methods for documentation and restoration of historic structures. In addition to traditional methods such as simple hand measurement and tachometry, terrestrial laser scanning is rapidly becoming one of the most commonly used techniques due to its completeness, accuracy and fastness characteristics. This study evaluates terrestrial laser scanning(TLS technology and photogrammetry for documenting the historical artifacts facade data in 3D Environment. PhotoModeler software developed by Eos System was preferred for Photogrammetric method. Leica HDS 6000 laser scanner developed by Leica Geosystems and Cyclone software which is the laser data evaluation software belonging to the company is preferred for Terrestrial Laser Scanning method. Taking into account the results obtained with this software product is intended to provide a contribution to the studies for the documentation of cultural heritage.

  6. [Intraoperative videoangiography using green indocyanine during aneurysm surgery].

    Science.gov (United States)

    Cordero, E; Enseñat, J; Macho, J; González, J J; Sánchez, M; Fernández, C; Caral, L; Valero, R; Ferrer, E

    2010-08-01

    The authors' objective is to report the initial appreciations on the use of the intraoperative near-infrared indocyanine green videoangiography during aneurysm surgery in our center. 10 surgical procedures have been made in 9 patients, 5 males and 4 females between 27 and 61 years old with an average of age of 49 years during a time of 10 months between March, 2008 and January, 2009. 10 surgical procedures were performed and 11 aneurysms were clipped. Intravenous indocyanine green and surgical microscope Leica OH4 with module of vascular fluorescence intraoperating Leica FL800, with camera infrared Sony (Heerbrugg-Switzerland) were used. The information offered by this technique during the intervention is compared with the images of the postoperative angiography performed during the first 24 hours. The partial or complete occlusion and the respect to the near vessels were evaluated. The findings of the intraoperative videoangiography were the complete occlusion and absence of complications in all the cases. These results corresponded completely with the postoperative results of the angiography postoperative, except in a case where the angiography demonstrated vasoespasmo moderate without clinical repercussion that during the videoangiografía intraoperatoria was not perceived. Clinically no patient presented neurological added deficits. The intraoperative videoangiography is a tool of easy application that offers valuable information as for the complete occlusion of the aneurysm and the permeability of the adjacent vessels.

  7. Histological characterization of Sticholecitha serpentis Prudhoe, 1949 (digenea, bieriidae, sticholecithinae, parasite of Bothrops moojeni Hoge, 1966 (serpentes, viperidae

    Directory of Open Access Journals (Sweden)

    TB. Silva

    2005-12-01

    Full Text Available The aim of this study was to evaluate the morphology of the species Sticholecitha serpentis Prudhoe, 1949 by means of histological procedures. Helminths were previously fixed in Railliet & Henry solution, uncompressed and were included in 2-hydroxyethyl-methacrylate. Longitudinal and transverse serial sections with a 4-mum thickness were performed in a microtome (Leica RM 2165, stained with haematoxylin-eosin and then analyzed in a computerized image analysis system (Qwin Lite 2.5, Leica. Structures of systematic value, such as oral sucker, acetabulum, prepharynx, pharynx, esophagus, intestinal caeca, vitelline glands, ovary, uterus, cirrus pouch and testicles were described. Structures that were poorly visible in total preparations were also observed and described, such as efferent ducts, ejaculatory duct, prostate, seminal vesicle, seminal receptacle, Laurer’s channel, Mehlis’ gland, vitelline ducts, metraterm, genital atrium, digestive glands and excretory vesicle. We demonstrated that histological analysis can supply important data regarding the morphological characterization of S. serpentis and will be able to contribute to systematic studies of trematodes.

  8. Laser microdissection and DNA typing of cells from single hair follicles.

    Science.gov (United States)

    Di Martino, D; Giuffrè, G; Staiti, N; Simone, A; Todaro, P; Saravo, L

    2004-12-02

    Isolation and identification of single cells from tissue samples or smears assume a great relevance in pathological and forensic applications; in this latter field, the possibility to identify a specific genetic profile can be obtained by short tandem repeat (STR) typing, allowing to achieve a scientific proof important in law courts. It is well known that DNA extraction may be performed from several tissue fragments, blood traces, spermatozoa as well as telogen hair. However, in the last case, few follicle cells are coupled to a great amount of keratin reducing the efficiency of DNA amplification. Recently, the introduction of laser microdissection technique has greatly improved the capability to select single cells without any cross-contamination. In the present report, we have performed a laser microdissection using a Leica AS LMD (Leica Microsystems, Germany), utilized on cutting the telogen hair in order to exclusively collect the lower part of the follicle and reduce keratin contamination. In this way we can accurately extract an adequate amount of DNA, successfully typed by STR profile.

  9. Proposal for the award of a contract for the supply of portable 3-d measuring systems

    CERN Document Server

    1999-01-01

    This document concerns the award of a contract for the supply of portable 3-D measuring systems for checking the dimensions of the cold masses of the LHC superconducting dipole and quadrupole magnets. A call for tenders (IT-2680/LHC/LHC) was sent on 4 June 1999 to fifteen firms in six Member States, one firm in Japan and two firms in the USA. By the closing date, CERN had received four tenders. The Finance Committee is invited to approve the negotiation of a contract with the firm LEICA GEOSYSTEMS (CH) for the supply of five portable 3-D measuring systems for the LHC superconducting dipole and quadrupole magnets, for a total amount of 859 500 Swiss francs, not subject to revision, with an option for the supply of up to five additional 3-D measuring systems, for a total amount up to 859 500 Swiss francs, subject to revision for contractual deliveries after 31 December 2002, bringing the overall total amount to a maximum of 1 719 000 Swiss francs, subject to revision after 31 December 2002 for the option. LEICA...

  10. Oblique Multi-Camera Systems - Orientation and Dense Matching Issues

    Science.gov (United States)

    Rupnik, E.; Nex, F.; Remondino, F.

    2014-03-01

    The use of oblique imagery has become a standard for many civil and mapping applications, thanks to the development of airborne digital multi-camera systems, as proposed by many companies (Blomoblique, IGI, Leica, Midas, Pictometry, Vexcel/Microsoft, VisionMap, etc.). The indisputable virtue of oblique photography lies in its simplicity of interpretation and understanding for inexperienced users allowing their use of oblique images in very different applications, such as building detection and reconstruction, building structural damage classification, road land updating and administration services, etc. The paper reports an overview of the actual oblique commercial systems and presents a workflow for the automated orientation and dense matching of large image blocks. Perspectives, potentialities, pitfalls and suggestions for achieving satisfactory results are given. Tests performed on two datasets acquired with two multi-camera systems over urban areas are also reported.

  11. Laser Capture Microdissection of Epithelium from a Wound Healing Model for MicroRNA Analysis.

    Science.gov (United States)

    Simões, Alyne; Chen, Zujian; Zhao, Yan; Chen, Lin; Macias, Virgilia; DiPietro, Luisa A; Zhou, Xiaofeng

    2018-01-01

    MicroRNAs are ~22 nucleotide-long noncoding RNAs influencing many cellular processes (including wound healing) by their regulatory functions on gene expression. The ability to analyze microRNA in different cells at the wound site is essential for understanding the critical role(s) of microRNA during various phases of wound healing. Laser capture micro-dissection (LCM) is an effective method to distinguish between relevant and non-relevant cells or tissues and enables the researcher to obtain homogeneous, ultra-pure samples from heterogeneous starting material. We present here our protocol for procuring epithelial cells from a mouse wound healing model using a Leica LMD7000 Laser Microdissection system, as well as the RNA isolation and downstream microRNA analysis. Using this method, researchers can selectively and routinely analyze regions of interest down to single cells to obtain results that are relevant, reproducible, and specific.

  12. SURVEY OF SELECTED PROCEDURES FOR THE INDIRECT DETERMINATION OF THE GROUP REFRACTIVE INDEX OF AIR

    Directory of Open Access Journals (Sweden)

    Filip Dvořáček

    2018-02-01

    Full Text Available The main aim of the research was to evaluate numeric procedures of the indirect determination of the group refractive index of air and to choose the suitable ones for requirements of ordinary and high accuracy distance measurement in geodesy and length metrology. For this purpose, 10 existing computation methods were derived from various authors’ original publications and all were analysed for wide intervals of wavelengths and atmospheric parameters. The determination of the phase and the group refractive indices are essential parts in the evaluation of the first velocity corrections of laser interferometers and electronic distance meters. The validity of modern procedures was tested with respect to updated CIPM-2007 equations of the density of air. The refraction model of Leica AT401 laser tracker was analysed.

  13. Evaluation of adaptation of ceramic inlays using optical coherence tomography and replica technique.

    Science.gov (United States)

    Ayse Gozde, Turk; Metin, Sabuncu; Mubin, Ulusoy

    2018-02-01

    Optical coherence tomography (OCT) has generally been used as a nondestructive technique to evaluate integrities of composite restorations. We investigated marginal and internal adaptations of ceramic inlay restorations with OCT and compared them to results with the silicone replica technique. Round-shaped class I cavities were prepared on 16 human maxillary first premolar teeth. Ceramic inlays were fabricated. Silicone replicas from inlays were obtained and sectioned to measure marginal and internal adaptations with a stereomicroscope (Leica Dfc 295, Bensheim, Germany). Inlays were cemented on respective teeth. Marginal and internal adaptations were then measured with the OCT system (Thorlabs, New Jersey, USA) in 200- μm intervals. Replica and OCT measurements were compared with independent samples t-tests. A paired t-test was used to evaluate the marginal and internal adaptations of each group (p inlays, whether measured by replica or OCT techniques, were within clinically acceptable limits.

  14. Consideraciones sobre la exactitud de las redes de levantamiento topográfico

    Directory of Open Access Journals (Sweden)

    Yordanis Esteban Batista-Legrá

    2013-09-01

    Full Text Available Se evaluó la exactitud de las poligonales medidas en el yacimiento Camarioca Sur lo que permitió obtener nuevos parámetros técnicos para la red de densificación geodésica, teniendo en cuenta el empleo de estaciones totales. Se utilizaron como referencia las poligonales trazadas por los técnicos de GEOCUBA, validándolas con los softwares Topo6, Autocad Map y Autocad Civil 3D 2010. Se empleó el sistema de posicionamiento global (GPS Leica 1200 para el control de los trabajos, tomando como referencia los puntos de apoyo utilizados por GEOCUBA Oriente Norte. Se concluye que cuando se usan estaciones totales es posible fusionar las poligonales de primera y segunda categoría en un solo grupo en virtud de que la exactitud de las mediciones es mayor.

  15. Target Visualization at the National Ignition Facility

    Energy Technology Data Exchange (ETDEWEB)

    Potter, Daniel Abraham [Univ. of California, Davis, CA (United States)

    2011-01-01

    As the National Ignition Facility continues its campaign to achieve ignition, new methods and tools will be required to measure the quality of the targets used to achieve this goal. Techniques have been developed to measure target surface features using a phase-shifting diffraction interferometer and Leica Microsystems confocal microscope. Using these techniques we are able to produce a detailed view of the shell surface, which in turn allows us to refine target manufacturing and cleaning processes. However, the volume of data produced limits the methods by which this data can be effectively viewed by a user. This paper introduces an image-based visualization system for data exploration of target shells at the National Ignition Facility (NIF) at Lawrence Livermore National Laboratory. It aims to combine multiple image sets into a single visualization to provide a method of navigating the data in ways that are not possible with existing tools.

  16. Grain refinement and macrosegregation behavior of direct chill cast Al-Zn-Mg-Cu alloy under combined electromagnetic fields

    Directory of Open Access Journals (Sweden)

    Yu-bo Zuo

    2015-09-01

    Full Text Available The combined electromagnetic fields were achieved by the application of an alternating magnetic field and a stationary magnetic field and were used during direct chill (DC casting process to control the microstructure and macrosegregation of an Al-Zn-Mg-Cu alloy. Ingot microstructures were analyzed under an optical microscope (Leica DMR. The composition at different locations in the ingots was measured with inductively coupled plasma mass spectrometry (ICP method. The results showed that the grain structure is transformed from dendrite to equiaxed structure and significantly refined with the application of combined electromagnetic fields. The uniformity of microstructure is also greatly improved. The combined electromagnetic fields show a significant effect on the distribution of elements. The negative macrosegregation in the centre area of the ingot is obviously reduced.

  17. Comparison microscope identification of a cheese bitemark: a case report.

    Science.gov (United States)

    Bernitz, H; Kloppers, B A

    2002-06-01

    Police investigating the murder of a farmer recovered a piece of cheese containing bite- marks. The local dental practitioner used white plaster to make casts of the bitemarks in the cheese and also of the teeth of three suspects. The cheese specimen was retained by the police and seven months later the case was referred to the Forensic Odontology Unit at the University of Pretoria where a silicone rubber cast of the bitemarks in the cheese was made. A lack of concordant features present in a conventional pattern-associated comparison was overcome with the aid of a Leica DMC comparison microscope. Individual features observed under 6.3x magnification aided in the positive identification of the suspect, who when confronted with the evidence, admitted guilt at his first court appearance.

  18. Aspects Regarding the Tracking of the Behavior in Time of Vâlsan Dam, Arges County, Romania

    Directory of Open Access Journals (Sweden)

    Vlad PĂUNESCU

    2017-11-01

    Full Text Available This paper aims to highlight the behavior in time monitoring of a hydrotechnical construction by establishing the displacements compared to the base tranche and the previous tranche, processing these measurements and establishing conclusions on the behavior of the analyzed construction. The main purpose of this paper is to study if there are any displacements of the studied hydrotechnical construction between different measurements tranches. Field measurements were performed between September - December 2016, after which the data was processed at the office with specially developed programs for this purpose. For the micro-triangulation stage, respectively for measuring the directions and distances, were used Leica TS02 Plus R500 total stations. The measurement accuracy of this total station is 1” for directional determination and 1.5mm + 2ppm for distances. Tracking the behavior in time of hydrotechnical constructions are of great importance, because it can prevent many calamities like breaking the dam, floods etc.

  19. Vertical Displacements Analysis of Measurements Achieved by Laser Station

    Science.gov (United States)

    Sztubecki, Jacek; Bujarkiewicz, Adam; Mrowczynska, Maria

    2017-12-01

    Measurements of vertical displacements using geodetic methods consist in the determination of changes in the location of reference points on the object or of its whole components. Such measurements require a high accuracy which can be ensured by the application of appropriate measuring instruments and measurement technologies. Modern measurement methods enable us to undertake tasks which traditional methods could not handle, and the required precision is guaranteed. This article describes an analysis of measurements of vertical displacements taken by means of a coordinate laser station, Leica TDRA 6000, as an alternative for measurements performed by precise levelling. An original approach to the stabilisation of measurement points is presented and technical guidelines for measurements are provided. An analysis of the data obtained from the measurements allowed us to formulate conclusions regarding the measurement technology in question and the accuracy of the results.

  20. Static terrestrial laser scanning of juvenile understory trees for field phenotyping

    Science.gov (United States)

    Wang, Huanhuan; Lin, Yi

    2014-11-01

    This study was to attempt the cutting-edge 3D remote sensing technique of static terrestrial laser scanning (TLS) for parametric 3D reconstruction of juvenile understory trees. The data for test was collected with a Leica HDS6100 TLS system in a single-scan way. The geometrical structures of juvenile understory trees are extracted by model fitting. Cones are used to model trunks and branches. Principal component analysis (PCA) is adopted to calculate their major axes. Coordinate transformation and orthogonal projection are used to estimate the parameters of the cones. Then, AutoCAD is utilized to simulate the morphological characteristics of the understory trees, and to add secondary branches and leaves in a random way. Comparison of the reference values and the estimated values gives the regression equation and shows that the proposed algorithm of extracting parameters is credible. The results have basically verified the applicability of TLS for field phenotyping of juvenile understory trees.

  1. From Survey to FEM Analysis for Documentation of Built Heritage: the Case Study of Villa Revedin-Bolasco

    Science.gov (United States)

    Guarnieri, A.; Fissore, F.; Masiero, A.; Di Donna, A.; Coppa, U.; Vettore, A.

    2017-05-01

    In the last decade advances in the fields of close-range photogrammetry, terrestrial laser scanning (TLS) and computer vision (CV) have enabled to collect different kind of information about a Cultural Heritage objects and to carry out highly accurate 3D models. Additionally, the integration between laser scanning technology and Finite Element Analysis (FEA) is gaining particular interest in recent years for structural analysis of built heritage, since the increasing computational capabilities allow to manipulate large datasets. In this note we illustrate the approach adopted for surveying, 3D modeling and structural analysis of Villa Revedin-Bolasco, a magnificent historical building located in the small walled town of Castelfranco Veneto, in northern Italy. In 2012 CIRGEO was charged by the University of Padova to carry out a survey of the Villa and Park, as preliminary step for subsequent restoration works. The inner geometry of the Villa was captured with two Leica Disto D3a BT hand-held laser meters, while the outer walls of the building were surveyed with a Leica C10 and a Faro Focus 3D 120 terrestrial laser scanners. Ancillary GNSS measurements were also collected for 3D laser model georeferencing. A solid model was then generated from the laser global point cloud in Rhinoceros software, and portion of it was used for simulation in a Finite Element Analysis (FEA). In the paper we discuss in detail all the steps and challenges addressed and solutions adopted concerning the survey, solid modeling and FEA from laser scanning data of the historical complex of Villa Revedin-Bolasco.

  2. Inventory of Close-to-Nature Forests Based on the Combination of Airborne LiDAR Data and Aerial Multispectral Images Using a Single-Tree Approach

    Directory of Open Access Journals (Sweden)

    Ivan Sačkov

    2017-11-01

    Full Text Available This study is concerned with the assessment of application possibilities for remote sensing data within a forest inventory in close-to-nature forests. A combination of discrete airborne laser scanning data and multispectral aerial images separately evaluated main tree and forest stand characteristics (i.e., the number of trees, mean height and diameter, tree species, tree height, tree diameter, and tree volume. We used eCognition software (Trimble GeoSpatial, Munich, Germany for tree species classification and reFLex software (National Forest Centre, Zvolen, Slovakia for individual tree detection as well as for forest inventory attribute estimations. The accuracy assessment was conducted at the ProSilva demo site Smolnícka Osada (Eastern Slovakia, Central Europe, which has been under selective management for more than 60 years. The remote sensing data were taken using a scanner (Leica ALS70-CM and camera (Leica RCD30 from an average height of 1034 m, and the ground reference data contained the measured positions and dimensions of 1151 trees in 45 plots distributed across the region. This approach identified 73% of overstory and 28% of understory trees. Tree species classification within overstory trees resulted in an overall accuracy slightly greater than 65%. We also found that the mean difference between the remote-based results and ground data was −0.3% for tree height, 1.1% for tree diameter, and 1.9% for stem volume. At the stand level, the mean difference reached values of 0.4%, 17.9%, and −21.4% for mean height, mean diameter, and growing stock, respectively.

  3. Comparative Study of the Volumetric Methods Calculation Using GNSS Measurements

    Science.gov (United States)

    Şmuleac, Adrian; Nemeş, Iacob; Alina Creţan, Ioana; Sorina Nemeş, Nicoleta; Şmuleac, Laura

    2017-10-01

    This paper aims to achieve volumetric calculations for different mineral aggregates using different methods of analysis and also comparison of results. To achieve these comparative studies and presentation were chosen two software licensed, namely TopoLT 11.2 and Surfer 13. TopoLT program is a program dedicated to the development of topographic and cadastral plans. 3D terrain model, level courves and calculation of cut and fill volumes, including georeferencing of images. The program Surfer 13 is produced by Golden Software, in 1983 and is active mainly used in various fields such as agriculture, construction, geophysical, geotechnical engineering, GIS, water resources and others. It is also able to achieve GRID terrain model, to achieve the density maps using the method of isolines, volumetric calculations, 3D maps. Also, it can read different file types, including SHP, DXF and XLSX. In these paper it is presented a comparison in terms of achieving volumetric calculations using TopoLT program by two methods: a method where we choose a 3D model both for surface as well as below the top surface and a 3D model in which we choose a 3D terrain model for the bottom surface and another 3D model for the top surface. The comparison of the two variants will be made with data obtained from the realization of volumetric calculations with the program Surfer 13 generating GRID terrain model. The topographical measurements were performed with equipment from Leica GPS 1200 Series. Measurements were made using Romanian position determination system - ROMPOS which ensures accurate positioning of reference and coordinates ETRS through the National Network of GNSS Permanent Stations. GPS data processing was performed with the program Leica Geo Combined Office. For the volumetric calculating the GPS used point are in 1970 stereographic projection system and for the altitude the reference is 1975 the Black Sea projection system.

  4. Biopsy of Different Oral Soft Tissues Lesions by KTP and Diode Laser: Histological Evaluation

    Directory of Open Access Journals (Sweden)

    Umberto Romeo

    2014-01-01

    Full Text Available Introduction. Oral biopsy aims to obtain clear and safe diagnosis; it can be performed by scalpel or laser. The controversy in this latter application is the thermal alteration due to tissue heating. The aim of this study is the histological evaluation of margins of “in vivo” biopsies collected by diode and KTP lasers. Material and Methods. 17 oral benign lesions biopsies were made by diode 808 nm (SOL, DenMatItalia, Italy and KTP 532 nm (SmartLite, DEKA, Italy. Samples were observed at OM LEICA DM 2000; margin alterations were evaluated through Leica Application Suite 3.4. Results. Epithelial and connective damages were assessed for each pathology with an average of 0.245 mm and a standard deviation of ±0.162 mm in mucoceles, 0.382 mm ± 0.149 mm in fibromas, 0.336 mm ± 0.106 mm in hyperkeratosis, 0.473 mm ± 0.105 mm in squamous hyperplasia, 0.182 mm in giant cell granuloma, and 0.149 mm in melanotic macula. Discussion. The histologic aspect of lesions influenced the response to laser, whereas the greater inflammation and cellularity were linked with the higher thermal signs. Many artifacts were also associated to histologic procedures. Conclusion. Both tested lasers permitted sure histologic diagnosis. However, it is suggested to enlarge biopsies of about 0.5 mm, to avoid thermal alterations, especially in inflammatory lesions like oral lichen planus.

  5. Three-Dimensional Recording of Bastion Middleburg Monument Using Terrestrial Laser Scanner

    Science.gov (United States)

    Majid, Z.; Lau, C. L.; Yusoff, A. R.

    2016-06-01

    This paper describes the use of terrestrial laser scanning for the full three-dimensional (3D) recording of historical monument, known as the Bastion Middleburg. The monument is located in Melaka, Malaysia, and was built by the Dutch in 1660. This monument serves as a major hub for the community when conducting commercial activities in estuaries Malacca and the Dutch build this monument as a control tower or fortress. The monument is located on the banks of the Malacca River was built between Stadhuys or better known as the Red House and Mill Quayside. The breakthrough fort on 25 November 2006 was a result of the National Heritage Department through in-depth research on the old map. The recording process begins with the placement of measuring targets at strategic locations around the monument. Spherical target was used in the point cloud data registration. The scanning process is carried out using a laser scanning system known as a terrestrial scanner Leica C10. This monument was scanned at seven scanning stations located surrounding the monument with medium scanning resolution mode. Images of the monument have also been captured using a digital camera that is setup in the scanner. For the purposes of proper registration process, the entire spherical target was scanned separately using a high scanning resolution mode. The point cloud data was pre-processed using Leica Cyclone software. The pre-processing process starting with the registration of seven scan data set through overlapping spherical targets. The post-process involved in the generation of coloured point cloud model of the monument using third-party software. The orthophoto of the monument was also produced. This research shows that the method of laser scanning provides an excellent solution for recording historical monuments with true scale of and texture.

  6. Adjustment and transformation strategies of ItalPoS Permanent GNSS Network

    Directory of Open Access Journals (Sweden)

    Alessandro Capra

    2009-06-01

    Full Text Available A private network of 133 Global Navigation Satellite Systems (GNSS permanent sites, named ItalPoS (Italian
    Positioning Service and uniformly covering the entire Italian territory, was established in April 2006 by the Italian
    Division of Leica Geosystems S.p.A. This network also involves several GPS stations of the INGV (Italian
    National Institute of Geophysics and Volcanology RING (Real-time Integrated National Gps network and GPS
    stations from other public and private bodies. Indeed Leica Geosystems contributed with its experience and its
    products to these networks which currently fulfil the national coverage where there are no ItalPoS stations. For
    instance, the purpose of the ItalPoS network is mainly GNSS data distribution in terms of real time corrections
    and RINEX files for the post-processing.
    In order to compute and distribute to private and scientific users a set of adjusted coordinates of the ItalPoS network
    in the IGS05 and ETRF89-2000 reference frames, a dataset of RINEX of about 120 GPS permanent sites
    was created and processed, using the distributed sessions approach, by means of Gamit/Globk 10.3 GPS data
    processor, also using some SOPAC (Scripps Orbits and Permanent Array Center and EUREF (European Permanent
    Network solutions to improve the reference frame definition and the internal constraints.
    This paper describes all the strategies adopted to estimate coordinates and velocities of the ItalPoS Network in
    IGS05 and in ETRF89-2000 reference frames in the whole Italian territory.

  7. Osseointegration of standard and mini dental implants: a histomorphometric comparison.

    Science.gov (United States)

    Dhaliwal, Jagjit S; Albuquerque, Rubens F; Murshed, Monzur; Feine, Jocelyne S

    2017-12-01

    Mini dental implants (MDIs) are becoming increasingly popular for rehabilitation of edentulous patients because of their several advantages. However, there is a lack of evidence on the osseointegration potential of the MDIs. The objective of the study was to histomorphometrically evaluate and compare bone apposition on the surface of MDIs and standard implants in a rabbit model. Nine New Zealand white rabbits were used for the study to meet statistical criteria for adequate power. Total 18 3M ™ ESPE ™ MDIs and 18 standard implants (Ankylos ® Friadent, Dentsply) were inserted randomly into the tibia of rabbits (four implants per rabbit); animals were sacrificed after a 6-week healing period. The specimens were retrieved en bloc and preserved in 10% formaldehyde solution. Specimens were prepared for embedding in a light cure acrylic resin (Technovit 9100). The most central sagittal histological sections (30-40 μm thick) were obtained using a Leica SP 1600 saw microtome. After staining, the Leica DM2000 microscope was used, the images were captured using Olympus DP72 camera and associated software. Bone implant contact (BIC) was measured using Infinity Analyze software. All implants were osseointegrated. Histologic measures show mineralized bone matrix in intimate contact with the implant surface in both groups. The median BIC was 57.5% (IQR 9.0) in the MDI group and 55.0% (IQR 4.5) in the control group (P > 0.05, Mann-Whitney test). There were no statistical differences in osseointegration at 6 weeks between MDIs and standard implants in rabbit tibias. Based on these results, it is concluded that osseointegration of MDIs is similar to that of standard implants.

  8. High-resolution monitoring of fluvial bedrock erosion in a natural gorge

    Science.gov (United States)

    Beer, Alexander R.; Turowski, Jens M.

    2014-05-01

    Morphological evolution of terrestrial and planetary landscapes is of increasing interest in the geosciences. In mountainous regions stream development and stream shape as a consequence of the interaction of uplift and erosion is fundamental for surface formation. Bedrock stream sections are prevalent that are routings for water and sediments. Hence, the correct description of bedrock channel evolution is fundamental for landscape modelling. To analyse how in situ erosion rates depend on factors like discharge, sediment transport and topography, there is a need of highly resolved topographic field data that so far is not available. Here we present preliminary outcomes of a change detection study from the Gorner Gorge above Zermatt, Switzerland. The outflow of the Gorner glacier (the Gornera stream) is captured most of the time by a water intake for hydropower production. However this intake is flushed twice a day in summer to purge settled sediments. Then the Gornera, charged with erosive bedload, runs along its natural stream bed that cuts through a roche moutonnée. This bedrock section (25m long, 5m wide and 8m deep) was surveyed repeatedly twice a year benefiting from nearly dry bed conditions during water capturing. A Leica ScanStation C10 was used for capturing high density point clouds (aspired average point spacing 5mm) of the bedrock surfaces. Referencing each of the various scanning positions was conducted using Leica HDS targets attached to fixed anchor bolts in the bedrock, that were surveyed locally with a total station. Resulting DEMs were used to calculate DEMs of difference (DoDs) for the bedrock walls and a huge boulder residing on the gravel bed. Erosion rates are visualised and discussed in respect of to the local spatial arrangement of the bedrock to the stream flow and water level.

  9. Cost of the treatment of complications of unsafe abortion in public hospitals.

    Science.gov (United States)

    Naghma-e-Rehan

    2011-02-01

    To study the cost of induced abortions (unsafe abortion) and treatment of the complications of such abortions. Descriptive study was conducted between July, 2008 and December, 2008 in four tertiary care hospitals of Lahore: Sir Ganga Ram Hospital, Lady Wellingdon Hospital, General Hospital and Family Health Hospital of Family Planning Association of Pakistan. One hundred women admitted with complications of induced abortion were interviewed by lady doctors, who were trained to conduct the interviews. The data was collected through a structured pre-designed and pre-tested questionnaire approved by International Planned Parenthood Federation. Based on the information provided by the woman and/or her husband and hospital sources, the average cost of abortion was Rs.1686/-. The cost varied from Rs. 500/- to 7,000/-depending on the city, type of provider and facilities available at the clinic. The average cost of treating the complications was Rs. 4,197/- ranging from Rs. 1600/- to Rs 45,000/-. At the present exchange rate (September, 2009), the cost will be US$ 20/- to 560/-. Wide variation between two extremes was due to the type and extent of complications. Treatment for abortion-related complications consumes a large portion of hospital budgets for obstetrics and gynaecology and results in considerable mortality and morbidity. Due to restrictive laws and high cost of safe abortions, most women faced with an unwanted pregnancy resort to unsafe abortions.

  10. Height and weight distribution of lower-middle income group of radiation workers

    International Nuclear Information System (INIS)

    Datta, S.; Sharma, R.C.; Sunta, C.M.

    1982-01-01

    Workers in the nuclear industry who are occasionally exposed to a radioactive environment were monitored for possible internal contamination. Calibration of the detection equipment was carried out with the help of a phantom. It is imperative that the phantom should have the physical dimensions of the subjects being monitored. As a step towards evolving a reference phantom, the height and weight distribution of the workers has been studied. The subjects included in this study are from lower middle income group drawing salaries between Rs 500 to 1000 per month. Mean weight +- SD was found to be 56.2 +- 8.70 kg and height 167 +- 5.90 cm. these averages match well with the data given by certain Life Insurance Companies in India. Although mean weight was found to be appreciably higher than the value reported in 1966, based on autopsy data, the mean weight and height are much less than the reference man values adopted by International Commission on Radiological Protection (ICRP) which are based on western man (average weight = 70 kg., average height = 174 cm). (author)

  11. Debris flow cartography using differential GNSS and Theodolite measurements

    Science.gov (United States)

    Khazaradze, Giorgi; Guinau, Marta; Calvet, Jaume; Furdada, Gloria; Victoriano, Ane; Génova, Mar; Suriñach, Emma

    2016-04-01

    , limits the visibility of the satellites and thus, can result in meter-level errors while estimating the positions. We have conducted 2 measurements using various differential GNSS systems in March and in September of 2015. During these measurements we used Leica Viva GS14 receiver as a rover station, which was equipped with a GSM card to establish an internet connection in order to receive differential corrections from continuous GNSS networks. During the first campaign we have used the RTK positioning method using the SmartNet network (http://es.smartnet-eu.com) operated by Leica. This system had the advantage of transmitting differential corrections for GPS and GLONASS systems. During the second campaign, we have had an access to the ICGC (http://www.icc.cat) CatNet permanent GPS network, which only provides GPS satellite corrections. Here we present the analysis of the obtained precisions from these two RTK systems. Additionally, we have analyzed the geodetic data in a post-processing mode using the Leica Geo Office 8.4 software with IGS estimated final orbits. For this procedure, in addition to using the data from nearby CatNet CGPS stations, we have also used data from the base station(s) specifically setup near the study area during the campaign period. The work has been supported by the Spanish Ministry of Science and Innovation project CHARMA: CHAracterization and ContRol of MAss Movements. A Challenge for Geohazard Mitigation (CGL2013-40828-R) and RISKNAT group (2014GR/1243).

  12. Socioeconomics of long-term glaucoma therapy in India

    Directory of Open Access Journals (Sweden)

    Bhagabat Nayak

    2015-01-01

    Full Text Available Purpose: To determine the socioeconomic impact of long-term glaucoma therapy. Materials and Methods: One hundred and fifty consecutive glaucoma patients on medical therapy, following up at our glaucoma service for at least 6 months were recruited. A questionnaire regarding monthly income, cost of glaucoma medications prescribed, availability of medications, travel time, time spent in review clinics, compliance, education status, medical insurance and systemic or local side-effects was administered. Results: The patients seen at the tertiary government hospital had an average monthly income of Rs. 10,912/- (range: Rs. 500/- to Rs. 50,000/- with approximately 56% of the patients having an income of less than Rs. 5000/month. The expenditure on anti-glaucoma medications ranged from 0.3% in high income group to 123% of their monthly gross income in low income group (P < 0.0001. The total expenditure including travel, stay, and loss of wages of patients and accompanying persons ranged from 1.6% in high income group to 137% of the monthly income in low income group (P < 0.0001. Mean time required for a glaucoma clinic visit was 15.66 h, (range: 6-96 h/month. About 2.7% experienced systemic side-effects and 21.3% had complaints of ocular adverse effects. About 90% of the patients were compliant. 92% were not covered by any insurance plan/government reimbursement for their treatment. Conclusions: Medical therapy for glaucoma is an economic burden to many patients and should be individualized, according to the socioeconomic status, availability of drugs and the required distance to travel to reach the specialist clinics.

  13. Retrospective study of chikungunya outbreak in urban areas of India

    Science.gov (United States)

    Nagpal, B.N.; Saxena, Rekha; Srivastava, Aruna; Singh, Neeru; Ghosh, S.K.; Sharma, S.K.; Kumar, Ashwani; Kumar, Hemant; Sharma, Alok Suman; Chand, S.K.; Ojha, V.P.; Mohanty, S.S.; Mohanty, A.K.; Dasgupta, R.K.; Dhillon, G.P.S.; Dash, A.P.

    2012-01-01

    Background & objectives: A retrospective study on chikungunya outbreak in India in five States viz. Delhi, Madhya Pradesh, Orissa, Maharashtra and Kerala was conducted in 2007-2008 to know the distribution and determinants of chikungunya fever outbreak in India. Methods: On the basis of high and low incidence of chikungunya fever, two districts from each State and two wards from the selected district were taken for random selection of 1000 households from 10 districts and 5 States. Semi-structured questionnaires were administered to individuals, patients, qualified health professionals and to stakeholders for collecting information. Results: The educational background and occupation of the respondents showed variations across the study States. Only in high incidence ward of Maharashtra, water storage period for 3-6 days and emptying, drying of water containers on weekly basis was noted. The study through knowledge, attitude, belief, practice (KABP) obtained individual's perception of chikungunya fever, its prevention and control. Patients’ expenditure on treatment was mainly recorded less than Rs 500 across study States. Health facility survey obtained an overview of the capacity of local health facilities. Stakeholders’ perception regarding chikungunya fever was also noted. Interpretation & Conclusions: The study revealed differences in awareness of chikungunya, cause of the disease, vector responsible, mode of transmission, biting time and elimination of breeding of mosquitoes statistically significant among high and low incidence wards of all the States. Expenditure on treatment was independent of economically active status and loss of man-days across all the States. Education and occupation did not have any relation with emptying/drying of water containers in high incidence wards. Strengthening of surveillance, information, education and communication (IEC) activities along with case management facilities may be provided by the State health department for

  14. A new leech species of Helobdella (Hirudinea, Glossiphoniidae from San Carlos de Bariloche, Río Negro, Argentina Una especie nueva de sanguijuela del género Helobdella (Hirudinea, Glossiphoniidae de San Carlos de Bariloche, Río Negro, Argentina

    Directory of Open Access Journals (Sweden)

    Bettina S. Gullo

    2009-04-01

    Full Text Available A new freshwater leech species Helobdella fantasmae n. sp. is described. This description is based on the examination of 12 specimens collected in Laguna Fantasma, Bariloche (41° 05'S 71° 28'W, during December 2002. Leeches were found attached to submerged plants. They were relaxed with gradual addition of 70% ethanol, fixed in 10% formalin, stored in 70% ethanol and stained with borax carmine. Examination of external morphology, dissections and microphotographs were accomplished with a Leica Wild M3Z stereo microscope aided with an Olympus C-4000 digital camera. H. fantasmae n. sp. differs from other species of the genus by the presence of 1 pair of eyes on somite III, crop without gastric chambers, digitiform caeca and postcaeca., short sperm ducts reaching the back of somite XV, atrium pyriform, short ovisacs reaching somite XIII. This is the first record of leeches from an ephemeral wetland in North Patagonia. This finding expands current knowledge of the biodiversity of Hirudinea in South America, increasing the number of known Helobdella spp. from the Río Negro province (North Patagonia to 10 species.Se describe una especie de sanguijuela dulceacuícola Helobdella fantasmae n. sp. Esta descripción se basa en el examen de 12 ejemplares recolectados en la Laguna Fantasma, Bariloche (41° 05'S 71° 28'O durante diciembre de 2002. Los individuos se hallaron asociados a la vegetación sumergida. Fueron relajados con la adición gradual de etanol 70%, fijados en formalina al 10%, preservados en etanol 70% y teñidos con carmín borácico. El examen de la morfología externa, disecciones y microfotografías fueron realizados con la ayuda de un estereomicroscopio Leica Wild M3Z con cámara digital Olympus C-4000. H. fantasmae n. sp. difiere de otras especies del género por los siguientes caracteres: un par de ojos en el somito III, estómago recto sin cámaras, ciegos laterales ni postciegos, espermiductos cortos que descienden hasta el

  15. Influence of the power level of an ultra-sonic system on dental cavity preparation Influência do nível de potência de um sistema de ultra-som em preparos cavitários

    Directory of Open Access Journals (Sweden)

    Érika Botelho Josgrilberg

    2007-12-01

    Full Text Available The aim of this study was to evaluate the shape of dental cavities made with the CVDentus® system using different ultrasound power levels. One standard cavity was made on the buccal aspect of 15 bovine incisors with a CVDentus® cylindrical bur (82142. The sample was divided into three groups: G1 - ultrasound with power II; G2 - ultrasound with power III; and G3 - ultrasound with power IV. A standardizing device was used to obtain standardized preparations and ultrasound was applied during one minute in each dental preparation. The cavities were sectioned in the middle, allowing observation of the cavity’s profile with a magnifying glass, and width and depth measurement using the Leica Qwin program. The Kruskal-Wallis (p O objetivo deste estudo foi avaliar o formato dos preparos cavitários realizados com o sistema CVDentus® utilizando potências variadas do ultra-som. Uma cavidade padronizada foi realizada na face vestibular de 15 incisivos bovinos utilizando as pontas cilíndricas CVDentus® (82142. A amostra foi dividida em 3 grupos: G1 - ultra-som com potência II; G2 - ultra-som com potência III; e G3 - ultra-som com potência IV. Foi utilizada uma máquina padronizadora de preparos cavitários e o ultra-som foi aplicado durante 1 minuto em cada preparo. As cavidades foram seccionadas no centro, permitindo a visualização do perfil cavitário em uma lupa estereoscópica, e esse foi medido em largura e profundidade por meio do programa Leica Qwin. O teste estatístico Kruskal-Wallis (p < 0,05 e o método de Dunn demonstraram diferenças entre os formatos das cavidades produzidas com as potências III e IV. Entretanto, as cavidades realizadas com a potência III apresentaram dimensões semelhantes às da ponta utilizada. Concluiu-se que a potência indicada pelo fabricante (III é a mais adequada para uso do sistema CVDentus®.

  16. Comparative anatomy of leaves of Kalanchoe pinnata and K. crenata in sun and shade conditions, as a support for their identification

    Directory of Open Access Journals (Sweden)

    Nattacha S. Moreira

    2012-04-01

    Full Text Available Kalanchoe pinnata (Lam. Pers. and K. crenata (Andrews Haw., Crassulaceae, are popularly used in the treatment of many diseases. Their biological activities, such as anti-leishmaniasis and analgesic, can be useful in phytotherapy. Both species are often misidentified as the other, because of their similar popular uses and names, and the similar external morphology of the leaves. We investigated the existence of anatomical characters that will permit correct identification of the species grown in shade and in sun conditions. We also contribute with new observations on the leaf anatomy of K. pinnata and K. crenata. Fixed (FAA70 leaves were used, and their sections were embedded in Leica historesin. Hydathodes were observed in both species, and for the first time were anatomically described in K. crenata. The species showed anatomical differences in relation to the presence of epidermal idioblasts only in K. crenata, the different pattern of distribution of subepidermal idioblasts, and the presence of leaf buds only in K. pinnata.

  17. Elemental and topographical imaging of microscopic variations in deposition on NSTX-U and DIII-D samples

    Science.gov (United States)

    Skinner, C. H.; Kaita, R.; Koel, B. E.; Chrobak, C. P.; Wampler, W. R.

    2017-10-01

    Tokamak plasma facing components (PFCs) have surface roughness that can cause microscopic spatial variations in erosion and deposition and hence influence material migration. Previous RBS measurements showed indirect evidence for this but the spatial (0.5mm) resolution was insufficient for direct imaging. We will present elemental images at sub-micron resolution of deposition on NSTX-U and DiMES samples that show strong microscopic variations and correlate this with 3D topographical maps of surface irregularities. The elemental imaging is performed with a Scanning Auger Microprobe (SAM) that measures element-specific Auger electrons excited by an SEM electron beam. 3D topographical maps of the samples are performed with a Leica DCM 3D confocal light microscope and compared to the elemental deposition pattern. The initial results appear consistent with erosion at the downstream edges of the surface pores exposed to the incident ion flux, whereas the deeper regions are shadowed and serve as deposition traps. Support was provided through DOE Contract Numbers DE-AC02-09CH11466, DE-FC02-04ER54698 and DE-NA0003525.

  18. Emprego de estações totais robotizadas na automação, controle e aquisição de dados, voltado ao monitoramento de barragens

    Directory of Open Access Journals (Sweden)

    Marco Aurélio Debus Nadal

    2017-02-01

    Full Text Available Atualmente com a evolução do instrumental utilizado em levantamentos geodésicos, especialmente no que diz respeito a robotização de estações totais, é possível a utilização destes em monitoramento geodésico contínuo, principalmente em estruturas de grande porte. No presente trabalho foi abordada a maneira de se estabelecer comunicação e controle destes instrumentos de medição através do desenvolvimento de programas que permitiram o envio e o recebimento de dados, em uma comunicação entre a estação total robotizada e o computador. Foram testadas as interfaces de comunicação que o instrumento utilizado neste trabalho, dentre elas: conexão via cabo serial, Bluetooth®, e link de rádio. Também foi estudado o protocolo de comunicação específico para este instrumento, disponibilizado pelo fabricante. O objetivo foi o desenvolvimento de um programa de computador que permitisse o controle de uma estação total robotizada Leica TCRA1205, voltado a aplicações de monitoramento geodésico.

  19. a Study about Terrestrial Laser Scanning for Reconstruction of Precast Concrete to Support Qlassic Assessment

    Science.gov (United States)

    Aziz, M. A.; Idris, K. M.; Majid, Z.; Ariff, M. F. M.; Yusoff, A. R.; Luh, L. C.; Abbas, M. A.; Chong, A. K.

    2016-09-01

    Nowadays, terrestrial laser scanning shows the potential to improve construction productivity by measuring the objects changes using real-time applications. This paper presents the process of implementation of an efficient framework for precast concrete using terrestrial laser scanning that enables contractors to acquire accurate data and support Quality Assessment System in Construction (QLASSIC). Leica Scanstation C10, black/white target, Autodesk Revit and Cyclone software were used in this study. The results were compared with the dimensional of based model precast concrete given by the company as a reference with the AutoDesk Revit model from the terrestrial laser scanning data and conventional method (measuring tape). To support QLASSIC, the tolerance dimensions of cast in-situ & precast elements is +10mm / -5mm. The results showed that the root mean square error for a Revit model is 2.972mm while using measuring tape is 13.687mm. The accuracy showed that terrestrial laser scanning has an advantage in construction jobs to support QLASSIC.

  20. Developing Verification Systems for Building Information Models of Heritage Buildings with Heterogeneous Datasets

    Science.gov (United States)

    Chow, L.; Fai, S.

    2017-08-01

    The digitization and abstraction of existing buildings into building information models requires the translation of heterogeneous datasets that may include CAD, technical reports, historic texts, archival drawings, terrestrial laser scanning, and photogrammetry into model elements. In this paper, we discuss a project undertaken by the Carleton Immersive Media Studio (CIMS) that explored the synthesis of heterogeneous datasets for the development of a building information model (BIM) for one of Canada's most significant heritage assets - the Centre Block of the Parliament Hill National Historic Site. The scope of the project included the development of an as-found model of the century-old, six-story building in anticipation of specific model uses for an extensive rehabilitation program. The as-found Centre Block model was developed in Revit using primarily point cloud data from terrestrial laser scanning. The data was captured by CIMS in partnership with Heritage Conservation Services (HCS), Public Services and Procurement Canada (PSPC), using a Leica C10 and P40 (exterior and large interior spaces) and a Faro Focus (small to mid-sized interior spaces). Secondary sources such as archival drawings, photographs, and technical reports were referenced in cases where point cloud data was not available. As a result of working with heterogeneous data sets, a verification system was introduced in order to communicate to model users/viewers the source of information for each building element within the model.

  1. DEVELOPING VERIFICATION SYSTEMS FOR BUILDING INFORMATION MODELS OF HERITAGE BUILDINGS WITH HETEROGENEOUS DATASETS

    Directory of Open Access Journals (Sweden)

    L. Chow

    2017-08-01

    Full Text Available The digitization and abstraction of existing buildings into building information models requires the translation of heterogeneous datasets that may include CAD, technical reports, historic texts, archival drawings, terrestrial laser scanning, and photogrammetry into model elements. In this paper, we discuss a project undertaken by the Carleton Immersive Media Studio (CIMS that explored the synthesis of heterogeneous datasets for the development of a building information model (BIM for one of Canada’s most significant heritage assets – the Centre Block of the Parliament Hill National Historic Site. The scope of the project included the development of an as-found model of the century-old, six-story building in anticipation of specific model uses for an extensive rehabilitation program. The as-found Centre Block model was developed in Revit using primarily point cloud data from terrestrial laser scanning. The data was captured by CIMS in partnership with Heritage Conservation Services (HCS, Public Services and Procurement Canada (PSPC, using a Leica C10 and P40 (exterior and large interior spaces and a Faro Focus (small to mid-sized interior spaces. Secondary sources such as archival drawings, photographs, and technical reports were referenced in cases where point cloud data was not available. As a result of working with heterogeneous data sets, a verification system was introduced in order to communicate to model users/viewers the source of information for each building element within the model.

  2. A STUDY ABOUT TERRESTRIAL LASER SCANNING FOR RECONSTRUCTION OF PRECAST CONCRETE TO SUPPORT QLASSIC ASSESSMENT

    Directory of Open Access Journals (Sweden)

    M. A. Aziz

    2016-09-01

    Full Text Available Nowadays, terrestrial laser scanning shows the potential to improve construction productivity by measuring the objects changes using real-time applications. This paper presents the process of implementation of an efficient framework for precast concrete using terrestrial laser scanning that enables contractors to acquire accurate data and support Quality Assessment System in Construction (QLASSIC. Leica Scanstation C10, black/white target, Autodesk Revit and Cyclone software were used in this study. The results were compared with the dimensional of based model precast concrete given by the company as a reference with the AutoDesk Revit model from the terrestrial laser scanning data and conventional method (measuring tape. To support QLASSIC, the tolerance dimensions of cast in-situ & precast elements is +10mm / -5mm. The results showed that the root mean square error for a Revit model is 2.972mm while using measuring tape is 13.687mm. The accuracy showed that terrestrial laser scanning has an advantage in construction jobs to support QLASSIC.

  3. The Segmentation of Point Clouds with K-Means and ANN (artifical Neural Network)

    Science.gov (United States)

    Kuçak, R. A.; Özdemir, E.; Erol, S.

    2017-05-01

    Segmentation of point clouds is recently used in many Geomatics Engineering applications such as the building extraction in urban areas, Digital Terrain Model (DTM) generation and the road or urban furniture extraction. Segmentation is a process of dividing point clouds according to their special characteristic layers. The present paper discusses K-means and self-organizing map (SOM) which is a type of ANN (Artificial Neural Network) segmentation algorithm which treats the segmentation of point cloud. The point clouds which generate with photogrammetric method and Terrestrial Lidar System (TLS) were segmented according to surface normal, intensity and curvature. Thus, the results were evaluated. LIDAR (Light Detection and Ranging) and Photogrammetry are commonly used to obtain point clouds in many remote sensing and geodesy applications. By photogrammetric method or LIDAR method, it is possible to obtain point cloud from terrestrial or airborne systems. In this study, the measurements were made with a Leica C10 laser scanner in LIDAR method. In photogrammetric method, the point cloud was obtained from photographs taken from the ground with a 13 MP non-metric camera.

  4. Ion projection lithography: November 2000 status and sub-70-nm prospects

    Science.gov (United States)

    Kaesmaier, Rainer; Wolter, Andreas; Loeschner, Hans; Schunck, Stefan

    2000-10-01

    Among all next generation lithography (NGL) options Ion Projection Lithography (IPL) offers the smallest (particle) wavelength of 5x10- 5nm (l00keV Helium ions). Thus, 4x reduction ion-optics has diffraction limits IOS) has been realized and assembled. In parallel to the PDT-IOS effort, at Leica Jena a test bench for a vertical vacuum 300mm-wafer stage has been realized. Operation of magnetic bearing supported stage movement has already been demonstrated. As ASML vacuum compatible optical wafer alignment system, with 3nm(3(sigma) ) precision demonstrated in air, has been integrated to this wafer test bench system recently. Parallel to the IPL tool development, Infineon Technologies Mask House and the Institute for Microelectronics Stuttgart are intensively working on the development of IPL stencil masks with success in producing 150mm and 200mm stencil masks as reported elsewhere. This paper is focused on information about the status of the PDT-IOS tool.

  5. Effect of SiO2 protective layer on the femtosecond laser-induced damage of HfO2/SiO2 multilayer high-reflective coatings

    International Nuclear Information System (INIS)

    Yuan Lei; Zhao Yuanan; Wang Congjuan; He Hongbo; Fan Zhengxiu; Shao Jianda

    2007-01-01

    Two kinds of HfO 2 /SiO 2 800 nm high-reflective (HR) coatings, with and without SiO 2 protective layer were deposited by electron beam evaporation. Laser-induced damage thresholds (LIDT) were measured for all samples with femtosecond laser pulses. The surface morphologies and the depth information of all samples were observed by Leica optical microscopy and WYKO surface profiler, respectively. It is found that SiO 2 protective layer had no positive effect on improving the LIDT of HR coating. A simple model including the conduction band electron production via multiphoton ionization and impact ionization is used to explain this phenomenon. Theoretical calculations show that the damage occurs first in the SiO 2 protective layer for HfO 2 /SiO 2 HR coating with SiO 2 protective layer. The relation of LIDT for two kinds of HfO 2 /SiO 2 HR coatings in calculation agrees with the experiment result

  6. FRACTAL ANALYSIS OF TRABECULAR BONE: A STANDARDISED METHODOLOGY

    Directory of Open Access Journals (Sweden)

    Ian Parkinson

    2011-05-01

    Full Text Available A standardised methodology for the fractal analysis of histological sections of trabecular bone has been established. A modified box counting method has been developed for use on a PC based image analyser (Quantimet 500MC, Leica Cambridge. The effect of image analyser settings, magnification, image orientation and threshold levels, was determined. Also, the range of scale over which trabecular bone is effectively fractal was determined and a method formulated to objectively calculate more than one fractal dimension from the modified Richardson plot. The results show that magnification, image orientation and threshold settings have little effect on the estimate of fractal dimension. Trabecular bone has a lower limit below which it is not fractal (λ<25 μm and the upper limit is 4250 μm. There are three distinct fractal dimensions for trabecular bone (sectional fractals, with magnitudes greater than 1.0 and less than 2.0. It has been shown that trabecular bone is effectively fractal over a defined range of scale. Also, within this range, there is more than 1 fractal dimension, describing spatial structural entities. Fractal analysis is a model independent method for describing a complex multifaceted structure, which can be adapted for the study of other biological systems. This may be at the cell, tissue or organ level and compliments conventional histomorphometric and stereological techniques.

  7. Histological Ex Vivo Evaluation of Peri-Incisional Thermal Effect Created by a New-Generation CO2 Superpulsed Laser

    Directory of Open Access Journals (Sweden)

    G. Palaia

    2014-01-01

    Full Text Available The purpose of this study is the evaluation of the histological effects of a new-generation superpulsed CO2 laser through an “ex vivo” study. A CO2 (λ = 10,600 nm ultra-speed laser (SmartUS20D, DEKA, Florence, Italy has been used at different parameters from 2 to 4 watt in Continuous Wave (CW and Pulsed Wave (PW, 50 Hz to obtain 30 samples from pig cadaver tongues. All the specimens have been subdivided into 6 groups (from A to F and each group consisted of 5 samples. A final specimen has been taken by scalpel and used as control group. Histological analysis has been performed using an optical microscope (Leica DM 2000 at a magnification of ×40. Results showed that histological readability was optimal in all the samples. The thermal damage has been negligible in all the groups. Furthermore, the average of thermal damage was 0,095 mm in the epithelial, while it was 0.245 mm in the connective tissue. Statistical analysis using Graphpad Prism 5 software showed no significant differences among the groups. CO2 laser demonstrated a good surgical effectiveness provoking little peripheral damage onto the cut edges and allowing a safe histological diagnosis.

  8. Penetration of a resin-based filling material into lateral root canals and quality of obturation by different techniques.

    Science.gov (United States)

    Michelotto, André Luiz da Costa; Moura-Netto, Cacio; Araki, Angela Toshie; Akisue, Eduardo; Sydney, Gilson Blitzkow

    2015-01-01

    The aim of this study was to evaluate the penetration of a resin/polyester polymer-based material (Resilon Real Seal; SybronEndo Corp., Orange, USA) into simulated lateral canals, and the quality of obturations by different techniques. A total of 30 standardized simulated canals were divided into three groups according to the technique of obturation used: MS (McSpadden), SB (SystemB/Obtura II), and LC (Lateral Condensation). To analyze the penetration of the filling material, the simulated canals were digitalized and the images were analyzed using the Leica QWIN Pro v2.3 software. The data of the middle and apical thirds were separately submitted to analysis of variance (ANOVA), followed by the Tukey's test for the comparison of the techniques. Results showed a significant difference (p test on interexaminer agreement and the nonparametric Kruskal-Wallis test indicated no significant difference between filling techniques. It was concluded that Resilon achieves greater levels of penetration when associated with thermoplastic obturation techniques.

  9. Cutting velocity accuracy as a criterion for comparing robot trajectories and manual movements for medical industry

    Science.gov (United States)

    Vorotnikov, A. A.; Klimov, D. D.; Romash, E. V.; Bashevskaya, O. S.; Poduraev, Yu. V.; Bazykyan, E. A.; Chunihin, A. A.

    2018-03-01

    Industrial robots perform technological operations, such as spot and arc welding, machining and laser cutting along different trajectories within their performance characteristics. The evaluation of these characteristics is carried out according to the criteria of the standard ISO 9283. The criteria of this standard are applicable in industrial manufacturing, but not in the medical industry, as they are not developed in the framework of medical tasks. Therefore, it is necessary to evaluate according to criteria built on different principles. In this article, the question of comparative evaluation of trajectories from program movements of a robot and manual movements of a surgeon, arising during the development of robotic medical complexes using industrial robots, is considered. A comparative evaluation is required to prove the expediency of automating medical operations in maxillofacial surgery. This study focuses on the estimation of velocity accuracy of a medical instrument. To obtain the velocity of the medical instrument, coordinates of the trajectory points from the program movements of the robot KUKA LWR4+ and trajectories from the manual movements of a professional surgeon have been measured. The measurement was carried out using a coordinate measuring machine, the laser tracker Leica LTD800. The accuracy estimation was carried out by two criteria: the criterion set out in the ISO 9283 standard, and the developed alternative criterion, the description of which is presented in this article. A quantitative comparative evaluation of the trajectories of a robot and a surgeon was obtained.

  10. Fluorescent dyes with large Stokes shifts for super-resolution optical microscopy of biological objects: a review.

    Science.gov (United States)

    Sednev, Maksim V; Belov, Vladimir N; Hell, Stefan W

    2015-10-22

    The review deals with commercially available organic dyes possessing large Stokes shifts and their applications as fluorescent labels in optical microscopy based on stimulated emission depletion (STED). STED microscopy breaks Abbe's diffraction barrier and provides optical resolution beyond the diffraction limit. STED microscopy is non-invasive and requires photostable fluorescent markers attached to biomolecules or other objects of interest. Up to now, in most biology-related STED experiments, bright and photoresistant dyes with small Stokes shifts of 20-40 nm were used. The rapid progress in STED microscopy showed that organic fluorophores possessing large Stokes shifts are indispensable in multi-color super-resolution techniques. The ultimate result of the imaging relies on the optimal combination of a dye, the bio-conjugation procedure and the performance of the optical microscope. Modern bioconjugation methods, basics of STED microscopy, as well as structures and spectral properties of the presently available fluorescent markers are reviewed and discussed. In particular, the spectral properties of the commercial dyes are tabulated and correlated with the available depletion wavelengths found in STED microscopes produced by LEICA Microsytems, Abberior Instruments and Picoquant GmbH.

  11. Moles, Laser, Mirrors and the Measurement of LHC Dipole Cold Masses

    CERN Document Server

    2002-01-01

    Marta Bajko measuring the geometry of a dipole magnet with the 3-D laser measuring machine. Over in buildings 181 and SM 18 the LHC dipole magnets await final measurements to make sure they are of exactly the right geometry (shape). And since the dipole cold masses are not straight (they are slightly bent in the horizontal plane), getting them to meet their very tight assembly tolerances of tenth of millimetre is not an easy task. 'We are aiming for one order of magnitude better than that which we actually need for the accuracy of the measurements, and old school rulers of any sort cannot handle these requirements,' says Marta Bajko, one of the project's engineers, 'for our work we need to rely on optical measuring technology.' And rely on optical measuring is exactly what they have done. The dipole measurements are being taken with a gadget called LTD 500 (Laser Tracker and Absolute Distance-Meter), created by Leica Geosystems, a Swiss Company that designs and distributes high precision measurement technol...

  12. An Integrated Method for Mapping Impervious and Pervious Areas in Urban Environments Using Hyperspectral and LiDAR Data

    Science.gov (United States)

    Hashemi Beni, L.; McArdle, S.; Khayer, Y.

    2014-11-01

    As urbanization continues to increase and extreme climatic events become more prevalent, urban planners and engineers are actively implementing adaptive measures to protect urban assets and communities. To support the urban planning adaptation process, mapping of impervious and pervious areas is essential to understanding the hydrodynamic environment within urban areas for flood risk planning. The application of advance geospatial data and analytical techniques using remote sensing and GIS can improve land surface characterization to better quantify surface run-off and infiltration. This study presents a method to combine airborne hyperspectral and LiDAR data for classifying pervious (e.g. vegetation, gravel, and soil) and impervious (e.g. asphalt and concrete) areas within road allowance areas for the City of Surrey, British Columbia, Canada. Hyperspectral data was acquired using the Compact Airborne Spectrographic Imager (CASI) at 1 m ground spatial resolution, consisting of 72 spectral bands, and LiDAR data acquired from Leica Airborne LiDAR system at a density of 20 points/m2. A spectral library was established using 10 cm orthophotography and GIS data to identify surface features. In addition to spectral functions such as mean and standard deviation, several spectral indices were developed to discriminate between asphalt, concrete, gravel, vegetation, and shadows respectively. A spectral analysis of selected endmembers was conducted and an initial classification technique was applied using Spectral Angle Mapper (SAM). The classification results (i.e. shadows) were improved by integrating LIDAR data with the hyperspectral data.

  13. Microscope-Integrated OCT Feasibility and Utility With the EnFocus System in the DISCOVER Study.

    Science.gov (United States)

    Runkle, Anne; Srivastava, Sunil K; Ehlers, Justis P

    2017-03-01

    To evaluate the feasibility and utility of a novel microscope-integrated intraoperative optical coherence tomography (OCT) system. The DISCOVER study is an investigational device study evaluating microscope-integrated intraoperative OCT systems for ophthalmic surgery. This report focuses on subjects imaged with the EnFocus prototype system (Leica Microsystems/Bioptigen, Morrisville, NC). OCT was performed at surgeon-directed milestones. Surgeons completed a questionnaire after each case to evaluate the impact of OCT on intraoperative management. Fifty eyes underwent imaging with the EnFocus system. Successful imaging was obtained in 46 of 50 eyes (92%). In eight cases (16%), surgical management was changed based on intraoperative OCT findings. In membrane peeling procedures, intraoperative OCT findings were discordant from the surgeon's initial impression in seven of 20 cases (35%). This study demonstrates the feasibility of microscope-integrated intraoperative OCT using the Bioptigen EnFocus system. Intraoperative OCT may provide surgeons with additional information that may influence surgical decision-making. [Ophthalmic Surg Lasers Imaging Retina. 2017;48:216-222.]. Copyright 2017, SLACK Incorporated.

  14. TESTING DIFFERENT SURVEY TECHNIQUES TO MODEL ARCHITECTONIC NARROW SPACES

    Directory of Open Access Journals (Sweden)

    A. Mandelli

    2017-08-01

    Full Text Available In the architectural survey field, there has been the spread of a vast number of automated techniques. However, it is important to underline the gap that exists between the technical specification sheet of a particular instrument and its usability, accuracy and level of automation reachable in real cases scenario, especially speaking about Cultural Heritage (CH field. In fact, even if the technical specifications (range, accuracy and field of view are known for each instrument, their functioning and features are influenced by the environment, shape and materials of the object. The results depend more on how techniques are employed than the nominal specifications of the instruments. The aim of this article is to evaluate the real usability, for the 1:50 architectonic restitution scale, of common and not so common survey techniques applied to the complex scenario of dark, intricate and narrow spaces such as service areas, corridors and stairs of Milan’s cathedral indoors. Tests have shown that the quality of the results is strongly affected by side-issues like the impossibility of following the theoretical ideal methodology when survey such spaces. The tested instruments are: the laser scanner Leica C10, the GeoSLAM ZEB1, the DOT DPI 8 and two photogrammetric setups, a full frame camera with a fisheye lens and the NCTech iSTAR, a panoramic camera. Each instrument presents advantages and limits concerning both the sensors themselves and the acquisition phase.

  15. Analysis of Deformations of the Skylight Construction at the Main Hall of the Warsaw University of Technology

    Science.gov (United States)

    Odziemczyk, Waldemar

    2015-02-01

    The paper presents technology and results of measurements of the steel construction of the skylight of the Main Hall of the Warsaw University of Technology. The new version of the automated measuring system has been used for measurements. This system is based on Leica TCRP1201+ total station and the TCcalc1200 software application, developed by the author, which operates on a laptop computer connected with the total station by the wire. Two test measurements were performed. Each of them consisted of cyclic measurement using the polar method, from one station; points located on the skylight construction, as well as control points located on concrete, bearing poles, were successively measured. Besides geometrical values (such as Hz, V angles and the slope distance D), the changes of temperature and atmospheric pressure, were also recorded. Processed results of measurements contained information concerning the behaviour of the skylight; asymmetry of horizontal displacements with respect to the X axis have been proved. Changes of parameters of the instrument telescope and changes of the instrument orientation were also stated; they were connected with changes of the temperature. The most important results of works have been presented in the form of diagrams.

  16. Fluorescent dyes with large Stokes shifts for super-resolution optical microscopy of biological objects: a review

    International Nuclear Information System (INIS)

    Sednev, Maksim V; Belov, Vladimir N; Hell, Stefan W

    2015-01-01

    The review deals with commercially available organic dyes possessing large Stokes shifts and their applications as fluorescent labels in optical microscopy based on stimulated emission depletion (STED). STED microscopy breaks Abbe’s diffraction barrier and provides optical resolution beyond the diffraction limit. STED microscopy is non-invasive and requires photostable fluorescent markers attached to biomolecules or other objects of interest. Up to now, in most biology-related STED experiments, bright and photoresistant dyes with small Stokes shifts of 20–40 nm were used. The rapid progress in STED microscopy showed that organic fluorophores possessing large Stokes shifts are indispensable in multi-color super-resolution techniques. The ultimate result of the imaging relies on the optimal combination of a dye, the bio-conjugation procedure and the performance of the optical microscope. Modern bioconjugation methods, basics of STED microscopy, as well as structures and spectral properties of the presently available fluorescent markers are reviewed and discussed. In particular, the spectral properties of the commercial dyes are tabulated and correlated with the available depletion wavelengths found in STED microscopes produced by LEICA Microsytems, Abberior Instruments and Picoquant GmbH. (topical review)

  17. Anatomical and micro-morphological analysis of the fruit and vegetative organs of Inula oculus-christi L. in the Pannonian part of Serbia

    Directory of Open Access Journals (Sweden)

    Karanović Dunja S.

    2016-01-01

    Full Text Available Inula oculus-christi L. is a perennial plant, 20-60 cm high, growing in south-east Europe, central and southern Russia and some parts of Asia. It belongs to Pontic-Pannonian floristic element. In Pannonian part of Serbia this species is extremely rare, with very few recently confirmed literature and herbarium data. According to the field data for the period from 2013 to 2015, only one population of this species was recorded in Pannonian part of Serbia, on the site of Rimski Šanac, which is threatened by anthropogenic activities. Furthermore, anatomical and micro-morphological features of I. oculus-christi have not been documented so far. Therefore, in order to get better knowledge of the biology this species, as well as on the basis of the abovementioned, its anatomical and micro-morphological characteristics were investigated. Cross sections of the leaf, stem, rhizome and fruit were obtained using cryotechnique procedure by Leica CM 1850 cryostat. Using a light microscopy, detailed descriptions of anatomical characteristics of the analysed organs were given. The scanning electron microscopy revealed specific qualitative features of leaf and fruit that characterize the species. Obtained data may be useful in determination of this species and represent the valuable contribution to its micro-morphological and anatomical differentiation from other congeneric and related species. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 173002

  18. Testing Different Survey Techniques to Model Architectonic Narrow Spaces

    Science.gov (United States)

    Mandelli, A.; Fassi, F.; Perfetti, L.; Polari, C.

    2017-08-01

    In the architectural survey field, there has been the spread of a vast number of automated techniques. However, it is important to underline the gap that exists between the technical specification sheet of a particular instrument and its usability, accuracy and level of automation reachable in real cases scenario, especially speaking about Cultural Heritage (CH) field. In fact, even if the technical specifications (range, accuracy and field of view) are known for each instrument, their functioning and features are influenced by the environment, shape and materials of the object. The results depend more on how techniques are employed than the nominal specifications of the instruments. The aim of this article is to evaluate the real usability, for the 1:50 architectonic restitution scale, of common and not so common survey techniques applied to the complex scenario of dark, intricate and narrow spaces such as service areas, corridors and stairs of Milan's cathedral indoors. Tests have shown that the quality of the results is strongly affected by side-issues like the impossibility of following the theoretical ideal methodology when survey such spaces. The tested instruments are: the laser scanner Leica C10, the GeoSLAM ZEB1, the DOT DPI 8 and two photogrammetric setups, a full frame camera with a fisheye lens and the NCTech iSTAR, a panoramic camera. Each instrument presents advantages and limits concerning both the sensors themselves and the acquisition phase.

  19. Uav-Based Automatic Tree Growth Measurement for Biomass Estimation

    Science.gov (United States)

    Karpina, M.; Jarząbek-Rychard, M.; Tymków, P.; Borkowski, A.

    2016-06-01

    Manual in-situ measurements of geometric tree parameters for the biomass volume estimation are time-consuming and economically non-effective. Photogrammetric techniques can be deployed in order to automate the measurement procedure. The purpose of the presented work is an automatic tree growth estimation based on Unmanned Aircraft Vehicle (UAV) imagery. The experiment was conducted in an agriculture test field with scots pine canopies. The data was collected using a Leica Aibotix X6V2 platform equipped with a Nikon D800 camera. Reference geometric parameters of selected sample plants were measured manually each week. In situ measurements were correlated with the UAV data acquisition. The correlation aimed at the investigation of optimal conditions for a flight and parameter settings for image acquisition. The collected images are processed in a state of the art tool resulting in a generation of dense 3D point clouds. The algorithm is developed in order to estimate geometric tree parameters from 3D points. Stem positions and tree tops are identified automatically in a cross section, followed by the calculation of tree heights. The automatically derived height values are compared to the reference measurements performed manually. The comparison allows for the evaluation of automatic growth estimation process. The accuracy achieved using UAV photogrammetry for tree heights estimation is about 5cm.

  20. The Electrophoretic Mobility of Proteins near Surfaces

    Science.gov (United States)

    Ramasamy, Perumal; Singh, Avtar; Rafailovich, Miriam; Sokolov, Jonathan

    2004-03-01

    We have attempted to apply the methods developed for surface DNA electrophoresis (1) for proteomics. Droplets of FITC stained Abumin, Poly- L-Lysine, or Casein purchased from Sigma were deposited on glass cover slips. The droplets were then place in contact with a TBE buffer solution contained in a cell molded from PDMS. Pt electrodes were inserted into the cell and a voltage was a applied. The motion of the protein was then imaged with a Leica Confocal microscope as a function of buffer concentration, distance from the surface, and applied voltage. The mobilities were then compared with those of uncharged one micron florescent Polystyrene beads. References: 1)Henzel WJ, Watanabe C, Stults JT., !0 Protein Identification: The Origins of Peptide Mass Fingerprinting. !1 J. American Society for Mass Spectrometry. 14 (September 2003): 931-942 2)Mathesius U, Imin N, Natera SH, Rolfe BG., !0 Proteomics as a functional genomics tool. !1 Methods of Molecular Biology 236: 395-414. *Work supported in part by the NSF-MRSEC program

  1. Comparison of the Selected State-Of-The-Art 3D Indoor Scanning and Point Cloud Generation Methods

    Directory of Open Access Journals (Sweden)

    Ville V. Lehtola

    2017-08-01

    Full Text Available Accurate three-dimensional (3D data from indoor spaces are of high importance for various applications in construction, indoor navigation and real estate management. Mobile scanning techniques are offering an efficient way to produce point clouds, but with a lower accuracy than the traditional terrestrial laser scanning (TLS. In this paper, we first tackle the problem of how the quality of a point cloud should be rigorously evaluated. Previous evaluations typically operate on some point cloud subset, using a manually-given length scale, which would perhaps describe the ranging precision or the properties of the environment. Instead, the metrics that we propose perform the quality evaluation to the full point cloud and over all of the length scales, revealing the method precision along with some possible problems related to the point clouds, such as outliers, over-completeness and misregistration. The proposed methods are used to evaluate the end product point clouds of some of the latest methods. In detail, point clouds are obtained from five commercial indoor mapping systems, Matterport, NavVis, Zebedee, Stencil and Leica Pegasus: Backpack, and three research prototypes, Aalto VILMA , FGI Slammer and the Würzburg backpack. These are compared against survey-grade TLS point clouds captured from three distinct test sites that each have different properties. Based on the presented experimental findings, we discuss the properties of the proposed metrics and the strengths and weaknesses of the above mapping systems and then suggest directions for future research.

  2. Tracing GFP-labeled WJMSCs in vivo using a chronic salpingitis model: an animal experiment.

    Science.gov (United States)

    Li, Zhe; Zhang, Zhao; Ming, Wai-Kit; Chen, Xin; Xiao, Xiao-Min

    2017-12-01

    The present study was conducted to evaluate the distribution of Wharton's jelly-derived mesenchymal stem cells (WJMSCs) and their repairing function on the oviduct. WJMSCs were transfected with the LV3-GFP-PURO lentivirus. Female New Zealand rabbits (n = 24) were divided randomly into control A and B groups and experimental C and D groups to establish inflammation models. Sterile saline solution or WJMSCs were injected into rabbits via ear veins and/or genital tract perfusion once weekly for 3 weeks. All rabbits were humanely sacrificed 1 week after the last perfusion to collect the oviduct, uterus, liver, and bladder for examination. Green fluorescent protein (GFP) and cytokeratin 7 (CK7) were imaged using a Leica Qwin Plus V3 fluorescence confocal microscope and analyzed as mean optical densities in an Image-Pro Plus analysis system. We found that lentivirus expressing the GFP gene produced an efficient transfection. The mean optical density values of GFP and CK7 in the oviducts were higher in the experimental D group than those in the control A and experimental C groups. No GFP fluorescence deposits occurred in the bladder of the control A group or experimental C group. Colocalization of CK7 and WJMSCs was observed in the oviducts in all groups. WJMSCs exhibited homing characteristics and migrated to the injured oviduct to promote epithelial cell growth. Additionally, local treatment resulted in higher efficiency.

  3. An immunohistochemical and fluorescence in situ hybridization-based comparison between the Oracle HER2 Bond Immunohistochemical System, Dako HercepTest, and Vysis PathVysion HER2 FISH using both commercially validated and modified ASCO/CAP and United Kingdom HER2 IHC scoring guidelines.

    LENUS (Irish Health Repository)

    O'Grady, Anthony

    2010-12-01

    Immunohistochemistry (IHC) is used as the frontline assay to determine HER2 status in invasive breast cancer patients. The aim of the study was to compare the performance of the Leica Oracle HER2 Bond IHC System (Oracle) with the current most readily accepted Dako HercepTest (HercepTest), using both commercially validated and modified ASCO\\/CAP and UK HER2 IHC scoring guidelines. A total of 445 breast cancer samples from 3 international clinical HER2 referral centers were stained with the 2 test systems and scored in a blinded fashion by experienced pathologists. The overall agreement between the 2 tests in a 3×3 (negative, equivocal and positive) analysis shows a concordance of 86.7% and 86.3%, respectively when analyzed using commercially validated and modified ASCO\\/CAP and UK HER2 IHC scoring guidelines. There is a good concordance between the Oracle and the HercepTest. The advantages of a complete fully automated test such as the Oracle include standardization of key analytical factors and improved turn around time. The implementation of the modified ASCO\\/CAP and UK HER2 IHC scoring guidelines has minimal effect on either assay interpretation, showing that Oracle can be used as a methodology for accurately determining HER2 IHC status in formalin fixed, paraffin-embedded breast cancer tissue.

  4. Integration of Geodata in Documenting Castle Ruins

    Science.gov (United States)

    Delis, P.; Wojtkowska, M.; Nerc, P.; Ewiak, I.; Lada, A.

    2016-06-01

    Textured three dimensional models are currently the one of the standard methods of representing the results of photogrammetric works. A realistic 3D model combines the geometrical relations between the structure's elements with realistic textures of each of its elements. Data used to create 3D models of structures can be derived from many different sources. The most commonly used tool for documentation purposes, is a digital camera and nowadays terrestrial laser scanning (TLS). Integration of data acquired from different sources allows modelling and visualization of 3D models historical structures. Additional aspect of data integration is possibility of complementing of missing points for example in point clouds. The paper shows the possibility of integrating data from terrestrial laser scanning with digital imagery and an analysis of the accuracy of the presented methods. The paper describes results obtained from raw data consisting of a point cloud measured using terrestrial laser scanning acquired from a Leica ScanStation2 and digital imagery taken using a Kodak DCS Pro 14N camera. The studied structure is the ruins of the Ilza castle in Poland.

  5. INTEGRATION OF GEODATA IN DOCUMENTING CASTLE RUINS

    Directory of Open Access Journals (Sweden)

    P. Delis

    2016-06-01

    Full Text Available Textured three dimensional models are currently the one of the standard methods of representing the results of photogrammetric works. A realistic 3D model combines the geometrical relations between the structure’s elements with realistic textures of each of its elements. Data used to create 3D models of structures can be derived from many different sources. The most commonly used tool for documentation purposes, is a digital camera and nowadays terrestrial laser scanning (TLS. Integration of data acquired from different sources allows modelling and visualization of 3D models historical structures. Additional aspect of data integration is possibility of complementing of missing points for example in point clouds. The paper shows the possibility of integrating data from terrestrial laser scanning with digital imagery and an analysis of the accuracy of the presented methods. The paper describes results obtained from raw data consisting of a point cloud measured using terrestrial laser scanning acquired from a Leica ScanStation2 and digital imagery taken using a Kodak DCS Pro 14N camera. The studied structure is the ruins of the Ilza castle in Poland.

  6. Diffusion of Boron in Cobalt Sinters

    Directory of Open Access Journals (Sweden)

    Borowiecka-Jamrozek J.

    2013-12-01

    Full Text Available W pracy przedstawiono wyniki badan procesu dyfuzji w warstwach powierzchniowych uzyskanych w wyniku nasycenia borem spieków otrzymanych z proszku kobaltu gatunku Co Extrafine. Warstwy otrzymano przy zastosowaniu proszkowej metody borowania opartej na wykorzystaniu mieszaniny o składzie: B4C jako składnik podstawowy stanowiacy zródło boru, (NH4Cl + NaF aktywator i Al2O3 jako wypełniacz. Zachowane były nastepujace parametry procesu: temperatura 950°C, czas 6h i 12h. Badania obejmowały wyznaczenie współczynnika dyfuzji w oparciu o strukture, grubosc uzyskanych i czas nasycenia warstw. Obserwacji naniesionych warstw dokonano za pomoca mikroskopu optycznego Leica DM-4000. Zdjecia ujawniły dwufazowa strukture warstw borkowych. Badania rentgenowskie potwierdziły wystepowanie faz o składzie: CoB i Co2B. Zbudowano model dyfuzji atomów boru w strukturze kobaltu zakładajac dyfuzje reaktywna. Obliczono współczynnik dyfuzji w oparciu o model atomowy dyfuzji wykorzystujac potencjały oddziaływan pomiedzy atomami boru i kobaltu. Otrzymane wyniki porównano z danymi doswiadczalnymi dyfuzji boru w innych materiałach.

  7. Testing EDM of Total Stations

    Directory of Open Access Journals (Sweden)

    Cirbus Ján

    2001-03-01

    Full Text Available The paper is devoted to testing electrooptical distance measuring devices (EDM built in total stations, than can be used for various tasks in the contemporary geodetic works. A rich market offer and availability of these universal measuring systems with satisfying distance range, excellent accuracy and other parameters, make total stations as dominant terrestrial geodetic instruments.For succesfully applying these instruments, above all for relliable distance measurements, the stability of the modulation frequency is the most important pre-condition. In the article, therefore, there are given some methods to verify the modulation frequency stability. In addition, some ways for determining the EDM distance constant and periodical corrections of the phase measuring unit are introduced for 4 types of EDM : LEICA 1700L, TOPCON GTS6A, TOPCON GTS2, C.ZEISS ELTA50. It were also investigated their possibilities for precise distance survey. Values of the determined constants and periodical corrections are presented in Tab. 2.Based on the investigation results of the 4 EDM types and using the values m obtained for different distances S, equations of the a posteriori standard deviations in form : m = (a+b.S were derived too.

  8. Control Measurements of Crane Rails Performed by Terrestrial Laser Scanning.

    Science.gov (United States)

    Kregar, Klemen; Možina, Jan; Ambrožič, Tomaž; Kogoj, Dušan; Marjetič, Aleš; Štebe, Gašper; Savšek, Simona

    2017-07-20

    This article presents a method for measuring the geometry of crane rails with terrestrial laser scanning (TLS). Two sets of crane rails were divided into segments, their planes were adjusted, and the characteristic rail lines were defined. We used their profiles to define the positional and altitude deviations of the rails, the span and height difference between the two rails, and we also verified that they complied with the Eurocode 3 standard. We tested the method on crane rails at the hydroelectric power plant in Krško and the thermal power plant in Brestanica. We used two scanning techniques: "pure" TLS (Riegel VZ-400) and "hybrid" TLS (Leica MS50) scanning. This article's original contribution lies in the detailed presentation of the computations used to define the characteristic lines of the rails without using the numeric procedures from existing software packages. We also analysed the influence of segment length and point density on the rail geometry results, and compared the two laser scanning techniques. We also compared the results obtained by terrestrial laser scanning with the results obtained from the classic polar method, which served as a reference point for its precision.

  9. [Morphologic, morphometrical, and histological aspects of the eggs of four species of the genera Meccus (Hemiptera, Reduviidae, Triatominae)].

    Science.gov (United States)

    Obara, Marcos Takashi; Barata, José Maria Soares; da Silva, Nilza Nunes; Ceretti Júnior, Walter; Urbinatti, Paulo Roberto; da Rosa, João Aristeu; Jurberg, José; Galvão, Cleber

    2007-02-01

    The objective of this work was to analyze the morphological, morphometrical, and histological characteristics of eggs of four triatomine bugs species still not studied, in order to understand phylogenetic aspects and to facilitate parameters used in taxonomy, with the purpose of a specific and generic characterization of these vectors in public health. The eggs of each species had come from the collections of the Laboratory of Triatomine bugs and Culicid mosquitoes of the Faculdade de Saúde Pública - USP (Faculty of Public Health/USP). The morphologic studies were carried out through optic microscopy (OM) and scanning electronic microscopy (SEM). The eggs were measured with the help of the digital paquimeter Starrett 727. In relation to histological measures, Microtome Leica RM 2145 was used. Similarities were evidenced in the exocorial architecture of the eggs when they were studied by OM and SEM. This similarity among the eggs suggests a recent speciation, probably derived from common ancestry, representing a monophyletic group. Some structures were also detected by the histological cuts. The study has contributed to the magnification and recognition in generic and specific terms of the Triatominae subfamily. These new data will be able to subsidize a better understanding to determine roles for each vector species and to facilitate parameters to be used in taxonomy.

  10. Storm-time ionization enhancements at the topside low-latitude ionosphere

    Directory of Open Access Journals (Sweden)

    A. Dmitriev

    2008-05-01

    Full Text Available Ion density enhancements at the topside low-latitude ionosphere during a Bastille storm on 15–16 July 2000 and Halloween storms on 29–31 October 2003 were studied using data from ROCSAT-1/IPEI experiment. Prominent ion density enhancements demonstrate similar temporal dynamics both in the sunlit and in the nightside hemispheres. The ion density increases dramatically (up to two orders of magnitude during the main phase of the geomagnetic storms and reaches peak values at the storm maximum. The density enhancements are mostly localized in the region of a South Atlantic Anomaly (SAA, which is characterized by very intense fluxes of energetic particles. The dynamics of near-Earth radiation was studied using SAMPEX/LEICA data on >0.6 MeV electrons and >0.8 MeV protons at around 600 km altitude. During the magnetic storms the energetic particle fluxes in the SAA region and in its vicinity increase more than three orders of magnitude. The location of increased fluxes overlaps well with the regions of ion density enhancements. Two mechanisms were considered to be responsible for the generation of storm-time ion density enhancements: prompt penetration of the interplanetary electric field and abundant ionization of the ionosphere by enhanced precipitation of energetic particles from the radiation belt.

  11. High-Resolution Characterization of Intertidal Geomorphology by TLS

    Science.gov (United States)

    Guarnieri, A.; Vettore, A.; Marani, M.

    2007-12-01

    Observational fluvial geomorphology has greatly benefited in the last decades from the wide availability of digital terrain data obtained by orthophotos and by means of accurate airborne laser scanner data (LiDAR). On the contrary, the spatially-distributed study of the geomorphology of intertidal areas, such as tidal flats and marshes, remains problematic owing to the small relief characterizing such environments, often of the order of a few tens of centimetres, i.e. comparable to the accuracy of state-of-the-art LiDAR data. Here we present the results of Terrestrial Laser Scanner (TLS) acquisitions performed within a tidal marsh in the Venice lagoon. The survey was performed using a Leica HDS 3000 TLS, characterized by a large Field of View (360 deg H x 270 deg V), a low beam divergence (DSM and a DTM. This is important e.g. in eco-geomorphic studies of intertidal environments, where conventional LiDAR technologies cannot easily separate first and last laser returns (because of the low vegetation height) and thus provide models of the surface as well as of the terrain. Furthermore, the DTM is shown to provide unprecedented characterizations of marsh morphology, e.g. regarding the cross-sectional properties of small-scale tidal creeks (widths of the order of 10 cm), previously observable only through conventional topographic surveys, thus not allowing a fully spatially-distributed description of their morphology.

  12. Integrating a Web-Based Whole-Slide Imaging System and Online Questionnaires in a National Cytopathology Peer Comparison Educational Program in Taiwan.

    Science.gov (United States)

    Hang, Jen-Fan; Liang, Wen-Yih; Hsu, Chih-Yi; Lai, Chiung-Ru

    2015-01-01

    In a peer comparison educational program, transferring glass slides between laboratories and collecting responses are time- and cost-consuming. Integrating a web-based whole-slide imaging (WSI) system and online questionnaires may serve as a promising solution. Five gynecologic Papanicolaou-stained smears and 5 nongynecologic slides were selected. The 10 whole-slide images were acquired by a Leica SCN-400 system and released via an Aperio eSlide Manager. Online questionnaires generated by Google Forms with access to the 10 whole-slide images were released to all the practitioners in Taiwan by e-mail. After closing the program, an online posttest feedback survey was conducted. A total of 302 participants joined the gynecologic test, and 291 joined the nongynecologic test. The correct interpretation rates were 81.8-93.7% in the former and 28.5-93.1% in the latter. In the posttest feedback survey, there were 63.2% of the participants reporting first-time WSI experience, and 97.9% of them said they would like to participate in a similar program again. Integrating a web-based WSI system and online questionnaires is an easy method to access nationwide practitioners. Participants can make interpretations using WSI even without prior experience. The model is valuable for those who want to initiate a large-scale cytopathology peer comparison educational program. © 2015 S. Karger AG, Basel.

  13. A NEW APPROACH FOR THE SEMI-AUTOMATIC TEXTURE GENERATION OF THE BUILDINGS FACADES, FROM TERRESTRIAL LASER SCANNER DATA

    Directory of Open Access Journals (Sweden)

    E. Oniga

    2012-07-01

    Full Text Available The result of the terrestrial laser scanning is an impressive number of spatial points, each of them being characterized as position by the X, Y and Z co-ordinates, by the value of the laser reflectance and their real color, expressed as RGB (Red, Green, Blue values. The color code for each LIDAR point is taken from the georeferenced digital images, taken with a high resolution panoramic camera incorporated in the scanner system. In this article I propose a new algorithm for the semiautomatic texture generation, using the color information, the RGB values of every point that has been taken by terrestrial laser scanning technology and the 3D surfaces defining the buildings facades, generated with the Leica Cyclone software. The first step is when the operator defines the limiting value, i.e. the minimum distance between a point and the closest surface. The second step consists in calculating the distances, or the perpendiculars drawn from each point to the closest surface. In the third step we associate the points whose 3D coordinates are known, to every surface, depending on the limiting value. The fourth step consists in computing the Voronoi diagram for the points that belong to a surface. The final step brings automatic association between the RGB value of the color code and the corresponding polygon of the Voronoi diagram. The advantage of using this algorithm is that we can obtain, in a semi-automatic manner, a photorealistic 3D model of the building.

  14. THE SEGMENTATION OF POINT CLOUDS WITH K-MEANS AND ANN (ARTIFICAL NEURAL NETWORK

    Directory of Open Access Journals (Sweden)

    R. A. Kuçak

    2017-05-01

    Full Text Available Segmentation of point clouds is recently used in many Geomatics Engineering applications such as the building extraction in urban areas, Digital Terrain Model (DTM generation and the road or urban furniture extraction. Segmentation is a process of dividing point clouds according to their special characteristic layers. The present paper discusses K-means and self-organizing map (SOM which is a type of ANN (Artificial Neural Network segmentation algorithm which treats the segmentation of point cloud. The point clouds which generate with photogrammetric method and Terrestrial Lidar System (TLS were segmented according to surface normal, intensity and curvature. Thus, the results were evaluated. LIDAR (Light Detection and Ranging and Photogrammetry are commonly used to obtain point clouds in many remote sensing and geodesy applications. By photogrammetric method or LIDAR method, it is possible to obtain point cloud from terrestrial or airborne systems. In this study, the measurements were made with a Leica C10 laser scanner in LIDAR method. In photogrammetric method, the point cloud was obtained from photographs taken from the ground with a 13 MP non-metric camera.

  15. Two new Horaiclavus (Horaiclavidae, Conoidea) species from the Indo-Pacific region.

    Science.gov (United States)

    Bonfitto, Antonio; Morassi, Mauro

    2014-06-19

    The genus Horaiclavus includes eight Holocene Indo-Pacific species (Appeltans et al. 2012). Herein, we describe two new species that resemble members of this genus in some aspects of shell morphology, but otherwise show features that suggest that they differ from "typical" Horaiclavus species. Descriptions and measurements were based on shells oriented spire up with the aperture facing the viewer and made using a Leica MS 5 Stereomicroscope for incident light and a 10× ocular micrometer. Scanning electron microscope (SEM) micrographs were taken using a Hitachi S-2400. The classification adopted here follows Bouchet et al. (2011). The following abbreviations are used in the text: MZB = Museo di Zoologia dell'Università di Bologna; MNHN = Muséum National d'Histoire Naturelle, Paris, France; NHMUK = The Natural History Museum of United Kingdom [formerly British Museum (Natural History)], London, United Kingdom; ZMA = Naturalis Biodiversity Center, Leiden, the Netherlands; ZRC = Zoological Reference Collection, Raffles Museum of Biodiversity Research, Singapore; a = aperture length; b = shell width; l = length; a/l = ratio of aperture length to total shell length; b/l = ratio of shell breadth to total length; dd= dead collected specimen(s); stn. = station.

  16. Expressions of matrix metalloproteinase-2 and extracellular matrix metalloproteinase inducer in retinoblastoma

    Directory of Open Access Journals (Sweden)

    Yu-Hong Cheng

    2015-07-01

    Full Text Available AIM: To investigate expressions of matrix metalloproteinase-2(MMP-2and extracellular matrix metalloproteinase inducer(EMMPRINin retinoblastoma(Rband the relationships between MMP-2, EMMPRIN and tumor development.METHODS:Immunohistochemical technique was used to detect expressions of MMP-2 and EMMPRIN in 39 cases of paraffin embedded Rb samples. Quantitative analysis of expressions of MMP-2 and EMMPRIN were assessed by measuring the mean gray scale of Rb tissue with LEICA IM50 Color Pathologic Analysis System. The differences of expressions of MMP-2 and EMMPRIN in each clinical and pathological stage were statistically analyzed, and the same step was also undertaken to study the relationship between Rb with MMP-2 positive expression and that with EMMPRIN positive expression.RESULTS: The positive expression rate of MMP-2 was 90%(Gray value: 109.64±14.52; 35/39, and that of EMMPRIN was 85%(Gray value: 108.01±13.60; 33/39. The expressions of MMP-2 and EMMPRIN were significantly higher in tumors of glaucomatous stage(Gray value: 108.21±11.47 and 107.56±14.32than those in intraocular stage(Gray value: 121.13±11.32 and 119.34±12.66; PPPPPPCONCLUSION: The positive expression levels of MMP-2 and EMMPRIN may correlate with tumor infiltration and metastasis.

  17. The head-mounted microscope.

    Science.gov (United States)

    Chen, Ting; Dailey, Seth H; Naze, Sawyer A; Jiang, Jack J

    2012-04-01

    Microsurgical equipment has greatly advanced since the inception of the microscope into the operating room. These advancements have allowed for superior surgical precision and better post-operative results. This study focuses on the use of the Leica HM500 head-mounted microscope for the operating phonosurgeon. The head-mounted microscope has an optical zoom from 2× to 9× and provides a working distance from 300 mm to 700 mm. The headpiece, with its articulated eyepieces, adjusts easily to head shape and circumference, and offers a focus function, which is either automatic or manually controlled. We performed five microlaryngoscopic operations utilizing the head-mounted microscope with successful results. By creating a more ergonomically favorable operating posture, a surgeon may be able to obtain greater precision and success in phonomicrosurgery. Phonomicrosurgery requires the precise manipulation of long-handled cantilevered instruments through the narrow bore of a laryngoscope. The head-mounted microscope shortens the working distance compared with a stand microscope, thereby increasing arm stability, which may improve surgical precision. Also, the head-mounted design permits flexibility in head position, enabling operator comfort, and delaying musculoskeletal fatigue. A head-mounted microscope decreases the working distance and provides better ergonomics in laryngoscopic microsurgery. These advances provide the potential to promote precision in phonomicrosurgery. Copyright © 2011 The American Laryngological, Rhinological, and Otological Society, Inc.

  18. Visualization of Target Inspection data at the National Ignition Facility

    Energy Technology Data Exchange (ETDEWEB)

    Potter, D; Antipa, N

    2012-02-16

    As the National Ignition Facility continues its campaign to achieve ignition, new methods and tools will be required to measure the quality of the target capsules used to achieve this goal. Techniques have been developed to measure capsule surface features using a phase-shifting diffraction interferometer and Leica Microsystems confocal microscope. These instruments produce multi-gigabyte datasets which consist of tens to hundreds of files. Existing software can handle viewing a small subset of an entire dataset, but none can view a dataset in its entirety. Additionally, without an established mode of transport that keeps the target capsules properly aligned throughout the assembly process, a means of aligning the two dataset coordinate systems is needed. The goal of this project is to develop web based software utilizing WebGL which will provide high level overview visualization of an entire dataset, with the capability to retrieve finer details on demand, in addition to facilitating alignment of multiple datasets with one another based on common features that have been visually identified by users of the system.

  19. Morphometric Analysis of Longipalpis (Diptera: Psychodidae) Complex Populations in Mato Grosso do Sul, Brazil.

    Science.gov (United States)

    Santos, Mirella F C; Andrade Filho, José D; Fernandes, Carlos E S; Mateus, Nathália L F; Eguchi, Gabriel U; Fernandes, Wedson D; Brazil, Reginaldo P; Oliveira, Everton F; Oliveira, Alessandra G

    2015-05-01

    Owing to the existence of cryptic species that are difficult to distinguish morphologically, the search for new taxonomic characters and methods for identifying and classifying sand flies continues. Lutzomyia longipalpis (Lutz & Neiva, 1912) and Lutzomyia cruzi (Mangabeira, 1938) (Diptera: Psychodidae) are two such species that occur in sympatry in some regions of Mato Grosso do Sul State (MS). Twenty females and twenty males from each of the five populations of Lu. longipalpis and one population of Lu. cruzi from MS were examined. An outlying population of Lu. longipalpis from Estrela de Alagoas, State of Alagoas, was used to compare the degree of divergence among the groups in MS. Specimens were cleared, mounted on slides, identified, and measured using LAS-Leica. The principal component analysis of morphometric characters showed a high degree of variation among females, while males varied to a lower degree. The populations of Alagoas and Miranda demonstrated the greatest variation. The first region, Alagoas, is geographically distant from the others and occurs under distinctly different ecological conditions, which likely accounts for the variation. Further studies should be made to elucidate the factors that contribute to the differences found between the populations of MS. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  20. T.O.F. LASER SCANNER FOR THE SURVEYING OF STATUES: A TEST ON A REAL CASE

    Directory of Open Access Journals (Sweden)

    G. Artese

    2013-07-01

    Full Text Available The contribution regards the surveying of two statues of famous contemporary sculptors that have been placed in the central zone of Cosenza, which has been transformed in an open air museum. To realize a 3-D representation of the museum, different methodologies have been used, based on classical surveying (total stations and GNSS, image data and range data. The increasing performances of the new models of Time Of Flight (T.O.F. laser scanners allow to build accurate models also for medium-size objects; on the other hand, the recent techniques of 3D modeling enable the processing of large amount of data and the effective removal of noises. Thus, if an extreme accuracy is not required, one can think to use the T.O.F. laser scanner, also for the surveying of statues. For the acquisition of the surfaces of the statues, two different types of laser scanning have been used: the Leica Scan StationC10, based on Time Of Flight, and the Minolta VIVID 300 triangulation scanner. In the paper, the comparison between the results obtained by using the different techniques is described.

  1. Automated RNA In Situ Hybridization for 18 High Risk Human Papilloma Viruses in Squamous Cell Carcinoma of the Head and Neck: Comparison With p16 Immunohistochemistry.

    Science.gov (United States)

    Drumheller, Bradley; Cohen, Cynthia; Lawson, Diane; Siddiqui, Momin T

    2017-08-02

    Detection of human papilloma virus (HPV)-related head and neck squamous cell carcinoma (HNSCC) is important, as HPV-associated HNSCCs respond better to therapy. The RNAscope HPV-test is a novel RNA in situ hybridization (ISH) technique which strongly stains transcripts of E6 and E7 mRNA in formalin-fixed, paraffin-embedded tissue, with the potential to replace the indirect immunohistochemical (IHC) marker for p16 protein. A direct clinical comparison between p16 IHC and an automated RNA ISH using 18 probes has not been established. Samples from 27 formalin-fixed, paraffin-embedded HNSCC cases from the Emory University Hospital archives were stained using 18 individual RNA ISH probes for high-risk HPV (RNAscope 2.5 LS Probe ) on a Leica autostainer (Buffalo Grove, IL) and were compared with p16 IHC. Two pathologists reviewed and reached a consensus on all interpretations. The RNAscope technique was positive in 89% (24/27) and the p16 IHC was positive in 78% (21/27). The RNAscope was negative in 11.1% of samples (3/27) and the p16 IHC-negative in 22.2% (6/27). The RNA ISH detected 100% of the p16-positive IHC-stained slides and had a concordance of 88.9% (24/27). This easy to interpret automated staining method for 18 high-risk HPV genotypes is a feasible replacement for the indirect p16 IHC method.

  2. Comparative anatomy of leaves of Kalanchoe pinnata and K. crenata in sun and shade conditions, as a support for their identification

    Directory of Open Access Journals (Sweden)

    Nattacha S. Moreira

    2012-10-01

    Full Text Available Kalanchoe pinnata (Lam. Pers. and K. crenata (Andrews Haw., Crassulaceae, are popularly used in the treatment of many diseases. Their biological activities, such as anti-leishmaniasis and analgesic, can be useful in phytotherapy. Both species are often misidentified as the other, because of their similar popular uses and names, and the similar external morphology of the leaves. We investigated the existence of anatomical characters that will permit correct identification of the species grown in shade and in sun conditions. We also contribute with new observations on the leaf anatomy of K. pinnata and K. crenata. Fixed (FAA70 leaves were used, and their sections were embedded in Leica historesin. Hydathodes were observed in both species, and for the first time were anatomically described in K. crenata. The species showed anatomical differences in relation to the presence of epidermal idioblasts only in K. crenata, the different pattern of distribution of subepidermal idioblasts, and the presence of leaf buds only in K. pinnata.

  3. Easy and versatile adaptive optics setup with deformable lens for high-resolution microscopy

    Science.gov (United States)

    Pozzi, P.; Quintavalla, M.; Verstraete, H.; Bijlsma, H.; Bonora, S.; Verhaegen, M.

    2017-06-01

    It has been widely proven in literature that most optical microscopy techniques can greatly benefit from the application of adaptive optics correction of phase aberrations through an adaptive optical element, such as a deformable mirror or a spatial light modulator. However, adaptive optics is not yet widely adopted in the life sciences community, mostly due to the lack of adaptive commercial microscopy systems, and the inherent technical difficulty in modifying an existing microscopy setup to integrate an adaptive element, both on the software and hardware sides. We present a plug-and-play adaptive optics module for generic optical microscopes, based on a prototype refractive 18 actuators adaptive optical element, which can be inserted in any microscope between the objective and the microscope body. Correction is performed in a sensorless fashion, optimizing image quality metrics of the image presented to the user on screen. The results presented show how an end-user oriented commercial confocal laser scanning microscope (Leica SP5) can be upgraded with adaptive optics with minor hardware modifications, and no changes to the microscope control software.

  4. High-accuracy local positioning network for the alignment of the Mu2e experiment.

    Energy Technology Data Exchange (ETDEWEB)

    Hejdukova, Jana B. [Czech Technical Univ., Prague (Czech Republic)

    2017-06-01

    This Diploma thesis describes the establishment of a high-precision local positioning network and accelerator alignment for the Mu2e physics experiment. The process of establishing new network consists of few steps: design of the network, pre-analysis, installation works, measurements of the network and making adjustments. Adjustments were performed using two approaches. First is a geodetic approach of taking into account the Earth’s curvature and the metrological approach of a pure 3D Cartesian system on the other side. The comparison of those two approaches is performed and evaluated in the results and compared with expected differences. The effect of the Earth’s curvature was found to be significant for this kind of network and should not be neglected. The measurements were obtained with Absolute Tracker AT401, leveling instrument Leica DNA03 and gyrotheodolite DMT Gyromat 2000. The coordinates of the points of the reference network were determined by the Least Square Meth od and the overall view is attached as Annexes.

  5. T.O.F. Laser Scanner for the Surveying of Statues: a Test on a Real Case

    Science.gov (United States)

    Artese, G.; De Napoli, L.; Artese, S.

    2013-07-01

    The contribution regards the surveying of two statues of famous contemporary sculptors that have been placed in the central zone of Cosenza, which has been transformed in an open air museum. To realize a 3-D representation of the museum, different methodologies have been used, based on classical surveying (total stations and GNSS), image data and range data. The increasing performances of the new models of Time Of Flight (T.O.F.) laser scanners allow to build accurate models also for medium-size objects; on the other hand, the recent techniques of 3D modeling enable the processing of large amount of data and the effective removal of noises. Thus, if an extreme accuracy is not required, one can think to use the T.O.F. laser scanner, also for the surveying of statues. For the acquisition of the surfaces of the statues, two different types of laser scanning have been used: the Leica Scan StationC10, based on Time Of Flight, and the Minolta VIVID 300 triangulation scanner. In the paper, the comparison between the results obtained by using the different techniques is described.

  6. [AO distribution and fluorescence spectra in myoblasts and single muscle fibres].

    Science.gov (United States)

    Beliaeva, T N; Krolenko, S A; Leont'eva, E A; Mozhenok, T P; Salova, A V; Faddeeva, M D

    2009-01-01

    Using spectral scanning regime of Leica TCS SL confocal microscope, acridine orange (AO) fluorescence spectra in nuclei and cytoplasms of living myoblasts L6J1 and frog single muscle fibres have been studied. AO fluorescence spectra in salt solutions dependent on free AO concentrations and in AO complexes with DNA have also been obtained for comparison. Myoblasts nuclei fluoresced in green spectral region with maximum at approximately 530 nm (corresponding AO monomers fluorescence), nucleoli fluoresced most brightly. Nuclear chromatin fluoresced not uniformly in these cells. We saw similar to myoblasts AO emission in nucleoli and nuclei of frog single muscle fibres. The uniformed weak green fluorescence was observed for myoblast cytoplasm. As to the muscle fibres sarcoplasm, we saw also AO green fluorescence in A-discs. In myoblasts and muscle fibre cytoplasm we saw the fluorescent red, yellow and green granules which were acidic organelles. The comparison of AO fluorescence spectra in living cells with fluorescence spectra of different AO concentrations and complexes of AO with DNA in buffer solutions allows estimation of AO concentration in acidic granules which is of interest in the investigation of cellular organelles functions in the processes of intracellular transport, adaptation, apoptosis and a number of pathological conditions.

  7. HISTOMORFOMETRIA DO INTESTINO DELGADO DE RATOS SUBMETIDOS A DIFERENTES FONTES PROTÉICAS

    Directory of Open Access Journals (Sweden)

    M. CARUSO

    2009-01-01

    Full Text Available

    Foi estudada a histomorfometria da porção inicial do intestino delgado de ratos, após a administração de concentrado protéico de soja e de caseína como únicas fontes protéicas. Foram mensurados o comprimento (CV, área (AV das vilosidades e profundidade (PC e área (AC das criptas intestinais, através de microscópio óptico Leica Leitz DMRXE, acoplado com Câmera e Software Analisador de Imagens. Os resultados indicaram significativas diferenças entre os grupos avaliados, particularmente nos parâmetros comprimento e área das vilosidades, evidenciando do ponto de vista histomorfológico, as diferenças existentes na absorção das proteínas do leite e da soja.

  8. Terrestrial laser scanning in monitoring of anthropogenic objects

    Science.gov (United States)

    Zaczek-Peplinska, Janina; Kowalska, Maria

    2017-12-01

    The registered xyz coordinates in the form of a point cloud captured by terrestrial laser scanner and the intensity values (I) assigned to them make it possible to perform geometric and spectral analyses. Comparison of point clouds registered in different time periods requires conversion of the data to a common coordinate system and proper data selection is necessary. Factors like point distribution dependant on the distance between the scanner and the surveyed surface, angle of incidence, tasked scan's density and intensity value have to be taken into consideration. A prerequisite for running a correct analysis of the obtained point clouds registered during periodic measurements using a laser scanner is the ability to determine the quality and accuracy of the analysed data. The article presents a concept of spectral data adjustment based on geometric analysis of a surface as well as examples of geometric analyses integrating geometric and physical data in one cloud of points: cloud point coordinates, recorded intensity values, and thermal images of an object. The experiments described here show multiple possibilities of usage of terrestrial laser scanning data and display the necessity of using multi-aspect and multi-source analyses in anthropogenic object monitoring. The article presents examples of multisource data analyses with regard to Intensity value correction due to the beam's incidence angle. The measurements were performed using a Leica Nova MS50 scanning total station, Z+F Imager 5010 scanner and the integrated Z+F T-Cam thermal camera.

  9. Terrestrial laser scanning in monitoring of anthropogenic objects

    Directory of Open Access Journals (Sweden)

    Zaczek-Peplinska Janina

    2017-12-01

    Full Text Available The registered xyz coordinates in the form of a point cloud captured by terrestrial laser scanner and the intensity values (I assigned to them make it possible to perform geometric and spectral analyses. Comparison of point clouds registered in different time periods requires conversion of the data to a common coordinate system and proper data selection is necessary. Factors like point distribution dependant on the distance between the scanner and the surveyed surface, angle of incidence, tasked scan’s density and intensity value have to be taken into consideration. A prerequisite for running a correct analysis of the obtained point clouds registered during periodic measurements using a laser scanner is the ability to determine the quality and accuracy of the analysed data. The article presents a concept of spectral data adjustment based on geometric analysis of a surface as well as examples of geometric analyses integrating geometric and physical data in one cloud of points: cloud point coordinates, recorded intensity values, and thermal images of an object. The experiments described here show multiple possibilities of usage of terrestrial laser scanning data and display the necessity of using multi-aspect and multi-source analyses in anthropogenic object monitoring. The article presents examples of multisource data analyses with regard to Intensity value correction due to the beam’s incidence angle. The measurements were performed using a Leica Nova MS50 scanning total station, Z+F Imager 5010 scanner and the integrated Z+F T-Cam thermal camera.

  10. Automatic Object Extraction from Electrical Substation Point Clouds

    Directory of Open Access Journals (Sweden)

    Mostafa Arastounia

    2015-11-01

    Full Text Available The reliability of power delivery can be profoundly improved by preventing wildlife-related power outages. This can be achieved by insulating electrical substation components with non-conductive covers. The manufacture of custom-built covers requires as-built models of the salient components. This study presents new, automated methodology to recognize key components of electrical substations from 3D LiDAR data acquired using terrestrial laser scanning. The proposed methodology includes six novel algorithms to recognize key components (fence, cables, circuit breakers, bushings and bus pipes of electrical substations. Three datasets with different resolutions and configurations are used in this study. A Leica HDS 6100 laser scanner was used to acquire the first dataset and a Faro Focus3D laser scanner was employed to collect the second and third datasets. The obtained results indicate that 178 and 171 out of 181 electrical substation elements were successfully recognized in the first and second dataset, respectively, and 183 out of 191 components were identified in the third dataset. The results also demonstrate that an average 97.8% accuracy and average 98.8% precision at the point cloud level can be achieved.

  11. Maternal behaviour and feeding practices as determinants of childhood diarrhoea: some observations amongst rural Bengalee mothers.

    Science.gov (United States)

    Ghosh, S; Sengupta, P G; Mandal, S K; Manna, B; Sikder, S N; Sirkar, B K

    1994-01-01

    In India, epidemiologists followed 980 rural families with children less than 3 years old living near Calcutta in West Bengal to identify determinants related to maternal behavior and feeding practices of childhood diarrhea. They identified 570 families with diarrhea cases and 410 families with no diarrhea cases. Children with diarrhea were more likely to live in Kuchcha housing (44.7% vs. 33.9%; p = 0.0006), to have a family income of less than Rs.500/month (44.2% vs. 36.6%; p = 0.016) and a mother who was illiterate (53.5% vs. 45.4%; p = 0.013). Nondiarrheal families were more likely to have a sanitary latrine (63.9% vs. 50.5%; p = 0.000031) and have soap (for ablution, 22.9% vs. 14.4%; p = 0.0005 and, before food handling, 7.1% vs. 3%; p = 0.0046). Mothers with children who did not have diarrhea were more likely to space their births at least 4 years apart than those with children who did have diarrhea (20.5% vs. 14.7%; p = 0.018). Mothers with children who did not have diarrhea were also less likely to practice poor hygiene. Specifically, they would tend not to use leftover food for the next feeding (19.1% vs. 38%; p = 0.02), to have children whose body and clothes were dirty (19.1% vs. 40%; p = 0.01), to dispose of stools indiscriminately (55.3% vs. 73.7%; p = 0.02), to share a common latrine with other villagers (15.9% vs. 36.2%; p = 0.008), and to stop drinking water in a wide mouth container (66% vs. 84.8%; p = 0.008). Mothers with children who did not have diarrhea were also more likely to wash the container used for feeding the children with soap (48.9% vs. 30.4%; p = 0.03).

  12. Alcohol consumption practices amongst adult males in a rural area of Haryana

    Directory of Open Access Journals (Sweden)

    Sandeep Sachdeva

    2014-01-01

    Full Text Available Objective: To assess alcohol consumption practices amongst adult males in a rural block area. Materials and Methods: Using multi-stage random sampling frame, at least 12 households were identified from selected anganwadi centers (n = 27, and one resident adult male from each household was interviewed in confidence using pre-designed, pre-tested, semi-structured interview schedule after seeking informed consent. In households with more than one adult male respondent, the elder member was recruited. For operational purpose, a drinker was defined as a person who had consumed at least one alcoholic drink in 1 year immediately preceding the survey. Results: A total of 345 adult males were covered with a mean age of 46.6 (±14.2 years. Nearly 64.6% had 5 years of schooling, 46.4% were farmers, 54.2% lived in joint family system and 59.7% had monthly family income up to Rs 10,000. Nearly 150 (43.5% subjects had some correct knowledge regarding adverse effects of alcohol consumption. Of all the study subjects, 326 (94.4% males had consumed at least one alcohol-based drink and of which 66.3% consumed country liquor. Nearly 239 (73.3% drank at either their respective homes or their friends′ homes. Two hundred and eighty-one (86.2% procured alcohol from a shop within the village; average expenditure per month was Rs 500-900 for 132 (40.5% respondents; and 38.3% subjects did not consume any snacks with alcohol intake. Drinking had become a habit for 194 (59.5% males; however, based on self-assessment, 131 (40.2% considered alcohol consumption as a problem and intended to stop drinking. Conclusion: Within study limitations, high prevalence of alcohol consumption was noted in this study, which may not only eventually lead to health/social issues but also adversely influence the younger generation for easy and early uptake of alcohol.

  13. Organização celular dos testículos em Hylidae e Leptodactylidae, no Pantanal (Estado do Mato Grosso do Sul, Brasil - DOI: 10.4025/actascibiolsci.v31i4.3332 Cellular organization of the testes in Hylidae and Leptodactylidae, in the Pantanal (Mato Grosso do Sul State, Brazil - DOI: 10.4025/actascibiolsci.v31i4.3332

    Directory of Open Access Journals (Sweden)

    Ana Barbara dos Santos Rosa

    2009-08-01

    Full Text Available O objetivo deste trabalho foi analisar e comparar a organização das células germinativas em diferentes estádios de diferenciação celular nos machos adultos de Dendropsophus nanus (Boulenger, 1889, Pseudis limellum (Cope, 1862, Pseudis paradoxa (Linnaeus, 1758 e Scinax acuminatus (Cope, 1862, pertencentes à família Hylidae, e de Leptodactylus chaquensis (Cei, 1950 e L. podicipinus (Cope, 1862, pertencentes à família Leptodactylidae, coletados no Pantanal e na serra da Bodoquena, no Estado do Mato Grosso do Sul, Brasil. Os testículos foram retirados, fixados, desidratados em uma série alcoólica e incluídos em resina plástica do tipo methacrilato glicol (Historesin Leica®; cortados a 3 µm, corados com azul de toluidina 1% e observados no microscópio de luz. Constatou-se que os indivíduos da família Hylidae apresentam, em todas as épocas do ano, presença de todas as células de linhagem germinativa da espermatogênese. Já os indivíduos da família Leptodactylidae apresentam todas as células da linhagem germinativa somente no período chuvoso. As variações das características na organização dos epitélios seminíferos, assim como da evidente diferença na quantidade de espermatozoides no interior dos túbulos, demonstram que os anuros estudados apresentam diferentes formas de desenvolvimento da espermatogênese ao longo do ano: de forma cíclica para os da família Hylidae e acíclica, com liberação explosiva de espermatozoides, para os da família LeptodactylidaeThe objective of this work was to make a comparative analysis of germ cell organization at different stages of cellular differentiation in adult males of Dendropsophus nanus (Boulenger, 1889, Pseudis limellum (Cope, 1862, P. paradoxa (Linnaeus, 1758, and Scinax acuminatus (Cope, 1862, belonging to the family Hylidae; and Leptodactylus chaquensis (Cei, 1950 and L. podicipinus (Cope, 1862, belonging to the family Leptodactylidae, collected in the Pantanal and in

  14. Modelos experimentais de desnutrição e sua influência no trofismo cutâneo Experimental models of malnutrition and its effect on skin trophism

    Directory of Open Access Journals (Sweden)

    Saulo Nani Leite

    2011-08-01

    Full Text Available FUNDAMENTOS: A pele, para exercer suas funções, necessita de níveis adequados de nutrientes. OBJETIVO: Analisar o trofismo cutâneo de ratos nutridos e desnutridos por meio de dois modelos de desnutrição. MÉTODOS: No Modelo Marasmo, utilizaram-se 60 ratos Wistar em controle dietético, dos quais 30 foram selecionados aleatoriamente para receber metade da dieta diária durante 60 dias. No Modelo Gelatina, empregaram-se 60 ratos, dos quais 30 receberam dieta associada a proteína de baixa qualidade (gelatina durante 30 dias. Avaliou-se o estado nutricional dos animais por meio da massa corporal, dos sinais clínicos e da dosagem de albumina sérica. Após o período de desnutrição, fez-se a histologia da pele dos animais para análise da espessura da derme e epiderme com o software Leica Application Suite; nas lâminas coradas com tricrômio de Gomori, analisou-se a colagênese com o software ImageJ. RESULTADOS: A massa corporal dos animais desnutridos pelo marasmo e gelatina foi significativamente menor (pBACKGROUND: The skin requires adequate levels of nutrients to function properly. OBJECTIVE: To analyze skin trophism in well-nourished and undernourished rats using two models of malnutrition. METHODS: In the marasmus model, 60 Wistar rats were kept on a controlled diet, 30 being randomly selected to receive half the established diet for 60 days. In the gelatin model, 60 rats were used, 30 of which received a diet consisting of poor quality protein (gelatin for 30 days. The nutritional status of the animals was evaluated according to body mass index, clinical signs and serum albumin measurement. After the period of malnutrition, histology was performed on the animals' skin to analyze the thickness of the dermis and epidermis using the Leica Application Suite software. Collagen was analyzed on slides stained with Gömöri trichrome using the ImageJ software program. RESULTS: The body mass index of the malnourished animals in the marasmus

  15. Acompanhamento da icterícia neonatal em recém-nascidos de termo e prematuros tardios Follow-up of neonatal jaundice in term and late premature newborns

    Directory of Open Access Journals (Sweden)

    Fernando Perazzini Facchini

    2007-08-01

    Full Text Available OBJETIVO: Relatar os resultados de um projeto de acompanhamento de recém-nascidos de termo e próximos ao termo ictéricos no período neonatal. MÉTODOS: Foram encaminhados a ambulatório especializado neonatos com peso > 2.000 g e/ou idade gestacional > 35 semanas, cuja icterícia na alta foi avaliada inicialmente com o icterômetro de Ingram, Bilicheck® e, se indicado, com bilirrubinômetro Unistat (Leica. A bilirrubinemia destes recém-nascidos situava-se no ou acima do percentil 40 do nomograma elaborado por Bhutani. Todos recém-nascidos tratados com fototerapia durante internação foram reavaliados laboratorialmente 24 horas após suspensão do tratamento. A indicação de reinternação para tratamento fototerápico intensivo foi para paciente com nível > 20 mg/dL. RESULTADOS: De um total de 11.259 neonatos, 2.452 (21,8% foram encaminhados para acompanhamento, dos quais 87,2% (2.140 retornaram. Oitenta neonatos retornados foram reinternados. Dos 2.452 encaminhados para retorno, 180 (7,3% tinham bilirrubinemia > 15 mg/dL na alta. Destes, 151 retornaram para acompanhamento. Vinte (13,2% foram reinternados para tratamento. Do total de reinternados, dois recém-nascidos apresentaram nível > 25 mg/dL e nenhum > 30 mg/dL. Todos responderam rapidamente à fototerapia intensiva, e não houve necessidade de utilizar exsangüinotransfusões. CONCLUSÕES: Nossos resultados sugerem que o esquema adotado é eficiente na detecção e prevenção de hiperbilirrubinemias de risco para produzir encefalopatia bilirrubínica em recém-nascidos de termo e próximos ao termo.OBJECTIVE: To report on the results of a project following term and near term newborn infants who were jaundiced during the neonatal period. METHODS: Neonates were referred to the follow-up clinic with weight > 2,000 g and/or gestational age > 35 weeks, and jaundice at discharge was initially assessed with an Ingram icterometer or Bilicheck and, if indicated, with a Unistat

  16. Comparação entre a dosagem transcutânea e plasmática de bilirrubina Comparison of transcutaneous and plasma bilirubin measurement

    Directory of Open Access Journals (Sweden)

    Maria das Graças da Cunha Leite

    2007-06-01

    Full Text Available OBJETIVOS: Comparar dosagens transcutâneas de bilirrubina pelo Bilicheck com a dosagem plasmática capilar pelo bilirrubinômetro Unistat (Leica. MÉTODOS: Foram realizadas 200 dosagens concomitantes (transcutânea e plasmática, calculadas a correlação e concordância entre elas e feita avaliação da influência do peso de nascimento, raça, idade gestacional, idade pós-natal e uso de fototerapia. RESULTADOS: A correlação linear foi de 0,92, e a média da diferença entre as dosagens foi de 0,72 (±1,57 mg/dL, com intervalo de confiança em 95% de -2,42 a +3,86. A curva ROC realizada com a dosagem transcutânea em 14 mg/dL demonstrou melhor sensibilidade (88,2% e especificidade (97,8%, com valor preditivo positivo de 78,9%, valor preditivo negativo de 98,9 e área abaixo da curva de 0,98. CONCLUSÃO: A dosagem realizada pelo Bilicheck pode substituir a dosagem plasmática capilar até o valor de 14 mg/dL. Acima deste nível, deve ser considerada apenas como rastreador na seleção de pacientes que devem ser submetidos a dosagem sangüínea.OBJECTIVES: To compare transcutaneous bilirubin measurements made using Bilicheck equipment with assays of capillary plasma using the Unistat bilirubinometer (Leica. METHODS: Two hundred concomitant assays were performed (transcutaneous and in plasma, and the correlation and level of agreement between them was calculated. An assessment was also made of the influence of birth weight, skin color, gestational age, postnatal age and phototherapy. RESULTS: The linear correlation coefficient was 0.92, and the mean difference between assays was 0.72 (±1.57 mg/dL, with a 95% confidence interval from -2.42 to +3.86. The best of a series of ROC curves demonstrated that transcutaneous assays at 14 mg/dL offer the best sensitivity (88.2% and specificity (97.8%, with a positive predictive value of 78.9%, negative predictive value of 98.9 and are below the curve of 0.98. CONCLUSIONS: Assays performed using

  17. New technology for the emulsification of petroleum residuals in water; Nueva tecnologia para la emulsificacion de residuales del petroleo en agua

    Energy Technology Data Exchange (ETDEWEB)

    Peralta, M. Vita; Arriola, Alejandro M.; Sanchez S, Ramon; Manzanares P, Emilio; Romo M, Cesar A.; Yery de Coss P, R. [Instituto de Investigaciones Electricas, Temixco, Morelos (Mexico)

    2001-07-01

    It turns out of interest to Comision Federal de Electricidad and Petroleos Mexicanos to reduce costs of fuel oil, reducing the viscosity of the residues petroleum of high vacuum distillation emulsifying these with water. The parameters that define the characteristics of the emulsions are mainly the relation of their components, viscosity, size and drop distribution and its stability. The experimental work began producing emulsions in form of lots, using samples of approximately 500 milliliters of vacuum residue. In this first stage, at different concentrations, were examined eight different surfactants, as well as combinations among them, also seven different stabilizers were used. With these tests it has been managed to identify the concentrations, as well as the most adequate relations of surfactants and stabilizers for the production of emulsions of vacuum residues in water. The size and drop distribution has been determined by means of an optical LEICA microscope, model DMLS, that has adapted a photographic system in which an emulsion photo is obtained and, later, a system of analysis of images is used for obtaining of the values of the diameter of the drops present in the emulsion. Finally, by means of statistical software the average size of the particles is obtained, as well as the drop size distribution. The tests results of emulsions that remain fluid are shown and an example of the microstructure of an emulsion of vacuum residue-in-water is given. As well as an example of histogram of the drop size distribution of an emulsion of vacuum distillation-in-water, and another one of the viscosity in function of temperature at different cut efforts in one emulsion of vacuum distillate residue-in-water. [Spanish] Resulta de interes para la Comision Federal de Electricidad y Petroleos Mexicanos reducir costos del combustoleo, reduciendo la viscosidad de los residuos de vacio emulsionando estos en agua. Los parametros que definen las caracteristicas de las

  18. The impact of a port on the surrounding seashores based on the 13-year monitoring results

    Science.gov (United States)

    Tõnisson, Hannes; Orviku, Kaarel; Kont, Are

    2017-04-01

    The study was carried out in the vicinity of Sillamäe town and industrial port, located on the north-eastern coast of Estonia in the south-eastern part of the Gulf of Finland (The Baltic Sea). Sillamäe was potentially one of the most serious threats for the whole Baltic Sea environment. The town was founded together with the construction of a highly specialized chemical and metallurgy plant in 1946, where fuel rods and nuclear materials for the Soviet nuclear power plants and weapons were produced. The current study is focusing on the shore processes and the coastal sea fronting Sillamäe. The town is located east of the port. It is the region with the highest potential impact of the port. Until the town was founded and the factory with its nuclear waste depository was constructed, the shores near the town were described as one litho-dynamic system with a good natural balance. Major human influence (construction of the port, waste depository, etc.) and additionally climate warming has taken place since then. The shores in front of the nuclear waste depository are well protected today. However, the rapidly expanding port is the major obstacle of the longshore sediment transport since 2001. The aim of the study is to analyze the impact of the port to the changes in coastal evolution and sediment budget in the vicinity of the port based on the regular monitoring results. The dynamics of the seashores was assessed using remote methods and in situ measurements. Remote methods included the analyses of shoreline changes and changes in scarp positions in space and time using orthophotos. The study is also based on the measurements of scarp edges, shorelines and shore profiles conducted in 2004-2016. The measurements were carried out using Leica GS09 RTK-GPS and Leica level. The volume of sediments in the active zone of each profile was calculated. The active zone was defined as the zone from the mean shoreline to the elevation where storm waves were still able to

  19. Significado da presença de esquizócitos no sangue periférico de gestantes com pré-eclâmpsia Ming of the presence of schistocytes in blood smear of preeclamptic pregnat women

    Directory of Open Access Journals (Sweden)

    Rosângela de Fátima do Nascimento e Silva

    2008-08-01

    Full Text Available OBJETIVO: avaliar o significado da presença de esquizócitos em esfregaço de sangue periférico de gestantes com pré-eclâmpsia, identificando-os e correlacionando-os com outros marcadores de hemólise e da gravidade da doença. MÉTODOS: foram avaliadas 76 lâminas de esfregaço de sangue periférico de gestantes portadoras de pré-eclâmpsia. Após a realização do esfregaço, as lâminas foram submetidas ao corante de Leishman e armazenadas até a leitura, feita em microscópio modelo DLMB, da marca Leica, com aumento de 40 vezes e imersão em óleo. O microscópio era dotado de software Qwin Lite 2.5, que permitia gravar as imagens dos campos escolhidos em CD-ROM. Em cada lâmina foram contados dez campos com aproximadamente 100 eritrócitos. Foi considerada presença de esquizócitos (fragmento irregular ou em forma de capacete, de mordida ou triângulo quando a porcentagem dos mesmos era maior ou igual que 0,2%. A presença de esquizócitos foi correlacionada com outros marcadores de hemólise (hemoglobina, bilirrubina total, desidrogenase lática e reticulócitos, marcadores da pré-eclâmpsia (proteinúria e número de plaquetas e com a gravidade da pré-eclâmpsia. Para análise estatística foi utilizado o programa Statistical Package in Social Science (SPSS, versão 10.0, com valor de pPURPOSE: to evaluate the significance of schizocytes presence in peripheral blood smear of pregnant women with pre-eclampsia, identifying and correlating them with other markers of hemolysis and of the disease severity. METHODS: Seventh six glass slides of peripheral blood smear of pregnant women with pre-eclampsia have been evaluated. After the smear, the slides have been stained with Leishman's dye and stored till they were examined with a Leica, model DLMB microscope, provided with the Qwin Lite 2.5 software that made it possible to record the images of selected fields in CD-ROM. Ten fields with approximately 100 erythrocytes were counted in each

  20. Estimating the accuracy of vectors derived from open data

    Science.gov (United States)

    Nikolakopoulos, Konstantinos G.; Dimitropoulos, George

    2017-10-01

    Many geological applications like mapping, open quarry monitoring or even the study of tectonic structures, require a high resolution and a very accurate representation of the surface in order to minimize errors. High resolution satellite images or airphotos can provide the necessary basemap for such applications. Nevertheless these kinds of images are quite expensive and hard to get access to. Google Earth is easily accessible to the public, but the question that arises is, if those images can be as reliable as the original satellite images and if they can be used in their place for geological applications. The purpose of this study was to investigate Google Earth images reliability, measure their accuracy and find out if they can provide valid results and finally if they can be used for mapping applications. The region that was investigated is Markopoulo quarry in Attiki Peninsula Greece. Images were taken using Google Earth, one for each direction (North, South, East, and West) from 2004 to 2015. All the images were of the same magnification and same image quality. Subsequently, those images were georeferenced with the use of ArcGIS software. The georeference procedure was executed again using Erdas Imagine software for comparison purposes. A quickbird satellite image over the same area was orthorectified in Leica Photogrammetry Suite. From the orthorectified Quickbird image and the Google Earth images the road network was digitized and the derived vectors were compared in ARCGIS. The comparison showed remarkably low deviation which leads us to the conclusion that Google Earth images can be used as alternative basemap in many applications.

  1. Water content determination of soil surface in an intensive apple orchard

    Science.gov (United States)

    Riczu, Péter; Nagy, Gábor; Tamás, János

    2015-04-01

    Currently in Hungary, less than 100,000 hectares of orchards can be found, from which cultivation of apple is one of the most dominant ones. Production of marketable horticulture products can be difficult without employing advanced and high quality horticulture practices, which, in turn, depends on appropriate management and irrigation systems, basically. The got out water amount depend on climatic, edafic factors and the water demand of plants as well. The soil water content can be determined by traditional and modern methods. In order to define soil moisture content, gravimetry measurement is one of the most accurate methods, but it is time consuming and sometimes soil sampling and given results are in different times. Today, IT provides the farmers such tools, like global positioning system (GPS), geographic information system (GIS) and remote sensing (RS). These tools develop in a great integration rapidly. RS methods are ideal to survey larger area quick and accurate. Laser scanning is a novel technique which analyses a real-world or object environment to collect structural and spectral data. In order to obtain soil moisture information, the Leica ScanStation C10 terrestrial 3D laser scanner was used on an intensive apple orchard on the Study and Regional Research Farm of the University of Debrecen, near Pallag. Previously, soil samples from the study area with different moisture content were used as reference points. Based on the return intensity values of the laser scanner can be distinguished the different moisture content areas of soil surface. Nevertheless, the error of laser distance echo were examined and statistically evaluated. This research was realized in the frames of TÁMOP 4.2.4. A/2-11-1-2012-0001 "National Excellence Program - Elaborating and operating an inland student and researcher personal support system". The project was subsidized by the European Union and co-financed by the European Social Fund.

  2. Standardisation of homoeopathic drug: Plectranthus fruticosus

    Directory of Open Access Journals (Sweden)

    P Padma Rao

    2014-01-01

    Full Text Available Background: Plectranthus fruticosus (Benth. Wight ex Hook. f, syn. P. deccanicus Brinq. is an under shrub belonging to the family Lamiaceae. Leaves and young stems of this plant are used as medicine in Homoeopathy. Objective: To carry out pharmacognostic and physico - chemical studies to use authentic and correct species as standard raw materials to ensure purity, quality and its usefulness. Materials and Methods: The leaves and young aerial parts of Plectranthus fruticosus were fixed in formaldehyde: acetic acid alcohol (F.A.A, processed for microtomy (paraffin method, sectioned, stained and permanent slides prepared following Johansen. The microtome sections in T.S. and longitudinal section (L.S. were obtained at 6-8 um thickness on Leica RM 2155 microtome. The powder microscopy characters were studied by boiling the powdered drug in distilled water, stained in saffranin and mounted with glycerine. Photomicrography was done with Olympus BX 53 research trinocular microscope. Results: The leaves are large, ovate to cordate, thin, margins with rounded serrations, surface coarse with dense covering hair. Petiole is long and stem is quadrangular with nodes. Trichomes are predominantly uniseriate, macroform, conical besides few uniseriate filiform peltate and capitate hairs. Lamina is conspicuously thin. The hypodermal collenchyma is angularly thickened. Vascular bundles in midvein are arranged in an ′arc′. Petiole is oblong to rounded, in transection (T.S. and undulated. A ring of angular collenchyma is present. Cortical cells possess starch grains and crystals of calcium oxalate. A continuous vascular cylinder is present interrupted with 2-3 seriate medullary rays. The physicochemical properties and HPTLC fingerprints of this plant have been standardized. Conclusion: The powder microscopic features and organoleptic characters along with the anatomical and physico chemical studies including HPTLC fingerprints are diagnostic to establish the

  3. Expression of dog1 in low-grade fibromyxoid sarcoma: A study of 19 cases and review of the literature.

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    Vallejo-Benítez, Ana; Rodríguez-Zarco, Enrique; Carrasco, Sara Pabón; Pereira-Gallardo, Sofia; Brugal Molina, Javier; García-Escudero, Antonio; Robles Frías, Antonio; Marcilla, David; González-Cámpora, Ricardo

    2017-10-01

    DOG1 is a highly-sensitive marker often included in the immunohistochemical panel for the diagnosis of gastrointestinal stromal tumors (GISTs). Recent research has shown that DOG1 may also be expressed by low-grade fibromyxoid sarcomas (LGFMSs); this may give rise to diagnostic error when the sarcoma is located in the abdominal cavity. This paper reports on immnohistochemical expression of DOG1 in 19 LGFMSs using two different monoclonal antibodies: K9 (Leica, Novocastra Laboratories, Newcastle upon Tyne, UK) and SP31 (Thermo Scientific, Freemont, USA). All LGFMSs displayed the standard histological pattern of alternating myxoid and fibrous areas, low cellularity and bland spindle-cell morphology. Positive staining for MUC4 was observed in 18/19 cases (94.7%), while there was rearrangement of the FUS gene in 14/19 (73.7%) cases and of the EWR1 gene in 2/19 (10.5%). The sarcoma staining negative for MUC4 displayed FUS gene rearrangement. Whole-section immunohistochemistry revealed positive staining for DOG1 in 8/19 cases (42.1%), though only with clone K9. Cytoplasmic as well as membrane staining was observed in all cases; staining was focal (10-30%) and of varying intensity (1+ to 2+). In conclusion, DOG1 clone K9 exhibited low sensitivity (42.1%) for the diagnosis of LGFMS, although higher than clone SP31. Since the two clones display similar sensitivity and specificity for GIST diagnosis, SP31 would appear to be more specific for this purpose, since no reaction was observed here with LGFMS, a GIST-mimicking lesion. Copyright © 2017 Elsevier Inc. All rights reserved.

  4. Effect of Technical Quality of Thermomechanical Die Forging of AA2099 Alloy

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    Łukaszek-Sołek A.

    2014-10-01

    Full Text Available The paper presents the results of investigations of a multicomponent third-generation aluminium alloy, classified as AA2099. The actual forging conditions were determined basing on the assessment of the quality of side surface of specimens subjected to compression in Gleeble 3800 simulator and on flow curves of the alloy, as well as numerical modelling of forging process performed with application of QForm 3D v.7 software. Compression tests were realized at temperatures 400-500 °C, with a strain rate of 0.001-100 s-1, up to a specified constant true strain value of 0.9. Microstructure examination in as-delivered state was performed with application of Leica DM 4000M optical microscope. The obtained results of isothermal deformation of specimens were correlated with the analysis of a characteristic layered pancake-type microstructure. The simulation of die forging of a complex-shape forging (high-current contact tip used in power engineering at the temperature 500 °C, was performed. The shape of a forging makes it possible to fully analyse the influence of thermomechanical process conditions on technical quality of a product. The simulation of forging process showed full correctness of material flow, with no signs of instability. At the same time, the analysis of investigations allowed to prepare and realize the industrial forging trials for a forging of a very complex shape, in a single step, at the temperature 500 °C, with application of thermomechanical treatment. The forging attained high quality of shape and surface. Directional specimens were taken, in order to be subjected to microstructure examination and hardness testing. The data obtained from industrial tests, combined with the results of testing using Gleeble simulator as well as from numerical modelling, make up the guidelines for mechanical processing of AA2099 alloy at the temperatures 470-500 °C.

  5. ANALYZING SPECTRAL CHARACTERISTICS OF SHADOW AREA FROM ADS-40 HIGH RADIOMETRIC RESOLUTION AERIAL IMAGES

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    Y.-T. Hsieh

    2016-06-01

    Full Text Available The shadows in optical remote sensing images are regarded as image nuisances in numerous applications. The classification and interpretation of shadow area in a remote sensing image are a challenge, because of the reduction or total loss of spectral information in those areas. In recent years, airborne multispectral aerial image devices have been developed 12-bit or higher radiometric resolution data, including Leica ADS-40, Intergraph DMC. The increased radiometric resolution of digital imagery provides more radiometric details of potential use in classification or interpretation of land cover of shadow areas. Therefore, the objectives of this study are to analyze the spectral properties of the land cover in the shadow areas by ADS-40 high radiometric resolution aerial images, and to investigate the spectral and vegetation index differences between the various shadow and non-shadow land covers. According to research findings of spectral analysis of ADS-40 image: (i The DN values in shadow area are much lower than in nonshadow area; (ii DN values received from shadowed areas that will also be affected by different land cover, and it shows the possibility of land cover property retrieval as in nonshadow area; (iii The DN values received from shadowed regions decrease in the visible band from short to long wavelengths due to scattering; (iv The shadow area NIR of vegetation category also shows a strong reflection; (v Generally, vegetation indexes (NDVI still have utility to classify the vegetation and non-vegetation in shadow area. The spectral data of high radiometric resolution images (ADS-40 is potential for the extract land cover information of shadow areas.

  6. Selected Problems of Determining the Course of Railway Routes by Use of GPS Network Solution

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    Koc, Władysław; Specht, Cezary

    2011-09-01

    The main problem related to railroad surveying design and its maintenance is the necessity to operate in local geodetic reference systems caused by the long rail sections with straight lines and curvatures of the running edge. Due to that reason the geodetic railroad classical surveying methods requires to divide all track for a short measurement section and that caused additional errors. Development of the Global Navigational Satellite Systems (GNSS) positioning methods operating in the standardized World Geodetic System (WGS-84) allowed verification of capability of utilization GPS measurements for railroad surveying. It can be stated that implemented satellite measurement techniques opens a whole new perspective on applied research and enables very precise determination of data for railway line determining, modernization and design. The research works focused on implementation GNSS multi-receivers measurement positioning platform for projecting and stock-taking working based on polish active geodesic network ASG-EUPOS, as a reference frame. In order to eliminate the influence of random measurement errors and to obtain the coordinates representing the actual shape of the track few campaigns were realized in 2009 and 2010. Leica GPS Total station system 1200 SmartRover (with ATX1230 GG antennas) receivers were located in the diameter of the measurement platform. Polish Active Geodetic Network ASG-EUPOS was used as a reference network transmitted Real Time Kinematic Positioning Service according to RTCM 3.1 standard. Optimum time period were selected for GNSS campaign and testing area was chosen without large obstructions. The article presents some surveying results of the measurement campaigns and also discusses the accuracy of the course determination. Analyzes and implementation of results in railroad design process are also discussed.

  7. Comparison of radiological and morphologic assessments of myocardial bridges.

    Science.gov (United States)

    Ercakmak, Burcu; Bulut, Elif; Hayran, Mutlu; Kaymaz, Figen; Bilgin, Selma; Hazirolan, Tuncay; Bayramoglu, Alp; Erbil, Mine

    2015-09-01

    In this study we aimed to compare the findings of coronary dual-source computed tomography angiography of myocardial bridges with cadaveric dissections. Forty-one isolated, non-damaged fresh sheep hearts were used in this study. Myocardial bridges of the anterior interventricular branch of the left coronary artery were demonstrated and analyzed by a coronary dual-source computed tomography angiography. Dissections along the left anterior interventricular branch of the left coronary artery were performed by using Zeiss OPMI pico microscope and the length of the bridges were measured. The depths of the myocardial bridges were measured from the stained sections by using the light microscope (Leica DM 6000B). MBs were found in all 41 hearts (100%) during dissections. Dual-source computed tomography angiography successfully detected 87.8% (36 of the 41 hearts) of the myocardial bridges measured on left anterior interventricular branch of left coronary artery. The lengths of the myocardial bridges were found 5-40 and 8-50 mm with dissection and dual-source computed tomography angiography, respectively. And the depths were found 0.7-4.5 mm by dual-source computed tomography angiography and 0.745-4.632 mm morphologically. Comparison of the mean values of the lengths showed statistically significantly higher values (22.0 ± 8.5, 17.7 ± 7.7 mm, p = 0.003) for the dissections. Radiological assessment also effectively discriminated complete bridges from incomplete ones. Our study showed that coronary computed tomography angiography is reliable in evaluating the presence and depth of myocardial bridges.

  8. Tidal Bore detection in the Garonne River using high frequency GNSS data

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    Frappart, Frédéric; Roussel, Nicolas; Darrozes, José; Bonneton, Philippe; Bonneton, Natalie; Detandt, Guillaume; Perosanz, Felix; Loyer, Sylvain

    2016-04-01

    A tidal bore is a positive surge propagating upstream that may form when a rising tide with significant amplitude enters shallow, gently sloping and narrowing rivers. Tidal bores have a significant impact on the river ecosystem behavior, especially in terms of sediment transport. Most of the existing field studies were limited to visual observations. Only a few field experiments have been devoted to a quantitative study of the tidal bore dynamics. We carried out a field study in August, 2015, using a GNSS buoy to measure the tidal bore in the Garonne River (France) at Podensac located 140 km upstream of the estuary mouth. Precise Point Positioning and Differential GNSS techniques were used to determine the river surface height variations with a 20 Hz sampling rate. This site was selected owing to the presence of well-developed undular tidal bores and also because of the absence of any significant curvature of the river at this location, which limits the complexity of the tidal bore structure. The Gironde estuary is located in the Bay of Biscay, on the southwest coast of France, and is formed from the meeting of the rivers Dordogne and Garonne. In the Gironde mouth, the mean neap tidal range and mean spring tidal range is 2.5 m and 5 m, respectively. As the tide propagates upstream a marked ebbflood asymmetry occurs in the upper reaches of the estuary and the wave is amplified. This large amplitude tidal wave propagates in the Garonne and Dordogne rivers up to 160 km from the estuary mouth. Both GNSS buoy and reference station use a Leica AR10 antenna and GR25 receiver. Both stations (reference and buoy) acquired data with a 20 Hz sampling rate. GNSS data were processed using RTKLib. Results allowed to detect the the wave train of the tidal bore that caused an elevation of the surface of around 1.5 m. Comparisons were performed using acoustic data showing a good agreement between both sources of data.

  9. A Comparative Study of Precise Point Positioning (PPP Accuracy Using Online Services

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    Malinowski Marcin

    2016-12-01

    Full Text Available Precise Point Positioning (PPP is a technique used to determine the position of receiver antenna without communication with the reference station. It may be an alternative solution to differential measurements, where maintaining a connection with a single RTK station or a regional network of reference stations RTN is necessary. This situation is especially common in areas with poorly developed infrastructure of ground stations. A lot of research conducted so far on the use of the PPP technique has been concerned about the development of entire day observation sessions. However, this paper presents the results of a comparative analysis of accuracy of absolute determination of position from observations which last between 1 to 7 hours with the use of four permanent services which execute calculations with PPP technique such as: Automatic Precise Positioning Service (APPS, Canadian Spatial Reference System Precise Point Positioning (CSRS-PPP, GNSS Analysis and Positioning Software (GAPS and magicPPP - Precise Point Positioning Solution (magicGNSS. On the basis of acquired results of measurements, it can be concluded that at least two-hour long measurements allow acquiring an absolute position with an accuracy of 2-4 cm. An evaluation of the impact on the accuracy of simultaneous positioning of three points test network on the change of the horizontal distance and the relative height difference between measured triangle vertices was also conducted. Distances and relative height differences between points of the triangular test network measured with a laser station Leica TDRA6000 were adopted as references. The analyses of results show that at least two hours long measurement sessions can be used to determine the horizontal distance or the difference in height with an accuracy of 1-2 cm. Rapid products employed in calculations conducted with PPP technique reached the accuracy of determining coordinates on a close level as in elaborations which employ

  10. Thickness and marking quality of different occlusal contact registration strips

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    Maria Fernanda de Souza Mauá Serapião TOLEDO

    2014-12-01

    Full Text Available Objectives Evaluate the thickness and the marking quality of different occlusal contact registration strips (OCRS and a possible correlation between them. Material and Methods The following OCRS were selected: Accufilm II, BK20, BK21, BK22, BK23, BK28, and BK31. The thickness was measured in three points of the OCRS with an electronic measuring device (TESA, and the mean was calculated. To produce the marks on the strips, composite resin specimens were adapted to a universal testing machine (Versat 2000 with 40 kgf load cell at a speed of 1.0 mm/min. The mark images were photographed with a stereoscopic microscope (Stemi SV11 and processed and analyzed by the 550-Leica Qwin® analyzer. Results Values (μm found in the 1st and 2nd thickness measurements were: Accufilm II - 16.4 and 14.2; BK20 - 10.0 and 8.1; BK21 - 9.5 and 8.0; BK22 - 9.7 and 8.7; BK23 - 9.8 and 7.9; BK28 - 12.8 and 10.0; and BK31 - 8.4 and 8.0, respectively. The mean (mm2 values found in the mark areas were: Accufilm II - 0.078; BK20 - 0.035; BK21 - 0.045; BK22 - 0.012; BK23 - 0.022; BK28 - 0.024; and BK31 - 0.024. The results were submitted to the Kruskal-Wallis (p<0.05 and Pearson’s correlation tests. Conclusions Only in the 2nd measurement, the OCRS thickness observed was similar to the value indicated by the manufacturers; the Accufilm II and the BK28 strips showed the better marks; and no correlation was found between the thickness and the marking area.

  11. Dem Assessment Derived from Close Range Photogrammetry: a Case Study from Kadavur Area, Karur District, Tamil Nadu, India

    Science.gov (United States)

    Anbarasan, S.; Sakthivel, R.

    2012-07-01

    Close-Range Photogrammetry is an accurate, cost effective technique of collecting measurements of real world objects and conditions, directly from photographs. Photogrammetry utilizes digital images to obtain accurate measurements and geometric data of the object or area of interest, in order to provide spatial information for Engineering design, spatial surveys or 3D modeling. The benefits of close-range Photogrammetry over other field procedures are purported to be: Increased accuracy; complete as-built information; reduced costs; reduced on-site time; and effective for small and large projects. The same basic principle of traditional Aerial Photogrammetry can be applied to stereoscopic pictures taken from lower altitudes or from the ground. Terrestrial, ground-based, and close-range are all descriptive terms that refer to photos taken with an object-to-camera distance less than 300m (1000 feet). (Matthews, N.A, 2008). Close range Photogrammetry is a technique for obtaining the geometric information (e.g. position, distance, size and shape) of any object in 3D space that was imaged on the two dimensional (2D) photos, (Wolf, P.R, et.al, 2000) DEM Generation requires many processing and computation, such as camera calibration, stereo matching, editing, and interpolation. All the mentioned steps contribute to the quality of DEM. Image on close range Photogrammetry can be captured using three kind of camera: metric camera, semi-metric camera, and non-metric camera (Hanke, K., et.al, 2002). In this paper DEM quality assessed at Kadavur area, Karur district, Tamil Naudu, India using Close Range Photogrammetry technique, Commercial Digital Camera and Leica Photogrammetry Suite.

  12. GIS for the needs of the Radioactive Waste Repository Authority

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    Jitka Mikšová

    2007-06-01

    Full Text Available The Radioactive Waste Repository Authority (RAWRA is a state organisation responsible for the management of activities related to the disposal of all existing and future radioactive waste and spent nuclear fuel classed as a waste in Czech Republic. Worldwide, a deep geological repository is considered the highest degree of safety for a nuclear waste disposal. Such a repository has to be built in a stable geological environment ensuring the isolation of the stored radioactive waste from the surrounding environment for a long period of time. The selection of suitable site for the deep geological repository construction is a complicated and long term process. Considering this fact and also in respect to an assumed volume of varied datasets the GIS RAWRA was established to ensure convenient management and availability of data containing spatial information.The system is based on ESRI (ArcInfo including extensions, ArcSDE, ArcIMS, Leica Geosystems (Image Analysis and Microsoft software (MS SQL Server. Resulting datasets from six recommended potentially suitable sites for the location of a geological repository have been incorporated into the geodatabase to date. The necessary analysis was made using ESRI software tools and, in addition, custom applications were developed including the metadata editor, etc. This analysis was carried out with respect to existing geological and non-geological criteria defined for a nuclear waste repository. Finally, all six investigated sites with a total area of 240 km2 were reduced in area, each of them resulting in an area of approximately 10km2 for further detailed characterisation.

  13. Chemical composition of the essential oil and botanical study of the flowers of Mentha suaveolens.

    Science.gov (United States)

    El-Kashoury, El-Sayeda A; El-Askary, Hesham I; Kandil, Zeinab A; Salem, Mohamed A

    2014-06-01

    Herbal medicines play a paramount role in the treatment of wide range of diseases, so there is a growing need for their quality control and standardization. Traditionally, histological and morphological inspections have been the usual methods to authenticate herbs intended for medicinal applications. Mentha suaveolens Ehrh. (Lamiaceae) is native to Africa Temperate Asia and Europe and it's cultivated in Egypt. The macro- and micromorphology of the flowers of M. suaveolens Ehrh. cultivated in Egypt were studied to find the diagnostic characters of this species. In addition, the chemical composition of the essential oil of the flowers was also studied to define the chemotype of the plant. Photographs of macro- and micromorphology were taken using Casio and Leica DFC500 digital cameras, respectively. In addition, the essential oil was prepared by hydrodistillation followed by gas chromatographic/mass spectrometric (GC/MS) analysis for identification of its components. The macro- and micromorphological characteristics of M. suaveolens were determined. The yield of the essential oil obtained by hydrodistillation from M. suaveolens flowers was 1.7% calculated on dry weight basis. GC/MS analysis of the oil resulted in identification of 29 components, which amounted to 99.77% of the total oil composition. The major component was carvone (50.59%) followed by limonene (31.25%). The results obtained herein revealed for the macro, micromorphological and chemical composition characteristics of the flowers. The results of GC/MS analysis of the essential oil supported that M. suaveolens cultivated in Egypt could be categorized as carvone-rich chemotype since this compound pertained its high relative percentile.

  14. Comparison of glacier loss on Qori Kalis, Peru and Mt. Kilimanjaro, Tanzania over the last decade using digital photogrammetry and stereo analysis

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    Lamantia, K.

    2017-12-01

    Rising global temperatures have created cause for concern, particularly among those who study the world's glaciers. Given their high sensitivity to climate change tropical glaciers can be used not only as indicators of change but can provide information necessary for more accurate interpretations of the mechanisms driving climate change. In the past, measurements of glacier extent changes such as for the Qori Kalis Glacier in Peru have been based on terrestrial photography and hand-plotted photogrammetry. Recent technological advances now provide an opportunity to modify the way these glaciers are observed and measured. New developments have opened doors for digital photogrammetry software such as the Leica Photogrammetry Suite and stereo analyst from ERDAS, which offers stereoscopic tools with the ability to plot the ice extent in a three dimensional image. At least two images from different perspectives are required to create the file for stereo analysis. The resulting three-dimensional digital content will offer more flexibility in analysis, quantification, and visualization for better documentation of retreating glaciers. It is possible to produce both two-and three-dimensional surface area estimations for glaciers such as Qori Kalis and the Kilimanjaro ice fields. Beyond a surface area measurement, the software also possesses the capability to create contours for the surface of the glacier as well as view and analyze properties such as slope and aspect. The surface area measurements taken with the digital method are compared with the hand-plotted measurements made in the past and are found to be comparable. A comparison of glacier loss over time as well as a comparison between both tropical locations, will be presented and should provide better insight to the drivers that are influencing current glacier loss. Making the transition from terrestrial, to aerial, and now to satellite imagery provides a simpler method for accessing and assessing changes in

  15. Applying object-based image analysis and knowledge-based classification to ADS-40 digital aerial photographs to facilitate complex forest land cover classification

    Science.gov (United States)

    Hsieh, Yi-Ta; Chen, Chaur-Tzuhn; Chen, Jan-Chang

    2017-01-01

    In general, considerable human and material resources are required for performing a forest inventory survey. Using remote sensing technologies to save forest inventory costs has thus become an important topic in forest inventory-related studies. Leica ADS-40 digital aerial photographs feature advantages such as high spatial resolution, high radiometric resolution, and a wealth of spectral information. As a result, they have been widely used to perform forest inventories. We classified ADS-40 digital aerial photographs according to the complex forest land cover types listed in the Fourth Forest Resource Survey in an effort to establish a classification method for categorizing ADS-40 digital aerial photographs. Subsequently, we classified the images using the knowledge-based classification method in combination with object-based analysis techniques, decision tree classification techniques, classification parameters such as object texture, shape, and spectral characteristics, a class-based classification method, and geographic information system mapping information. Finally, the results were compared with manually interpreted aerial photographs. Images were classified using a hierarchical classification method comprised of four classification levels (levels 1 to 4). The classification overall accuracy (OA) of levels 1 to 4 is within a range of 64.29% to 98.50%. The final result comparisons showed that the proposed classification method achieved an OA of 78.20% and a kappa coefficient of 0.7597. On the basis of the image classification results, classification errors occurred mostly in images of sunlit crowns because the image values for individual trees varied. Such a variance was caused by the crown structure and the incident angle of the sun. These errors lowered image classification accuracy and warrant further studies. This study corroborates the high feasibility for mapping complex forest land cover types using ADS-40 digital aerial photographs.

  16. Evaluation of adaptation of ceramic inlays using optical coherence tomography and replica technique

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    TURK Ayse Gozde

    2018-02-01

    Full Text Available Abstract Optical coherence tomography (OCT has generally been used as a nondestructive technique to evaluate integrities of composite restorations. We investigated marginal and internal adaptations of ceramic inlay restorations with OCT and compared them to results with the silicone replica technique. Round-shaped class I cavities were prepared on 16 human maxillary first premolar teeth. Ceramic inlays were fabricated. Silicone replicas from inlays were obtained and sectioned to measure marginal and internal adaptations with a stereomicroscope (Leica Dfc 295, Bensheim, Germany. Inlays were cemented on respective teeth. Marginal and internal adaptations were then measured with the OCT system (Thorlabs, New Jersey, USA in 200- μm intervals. Replica and OCT measurements were compared with independent samples t-tests. A paired t-test was used to evaluate the marginal and internal adaptations of each group (p < 0.05. Marginal and internal adaptations were 100.97 ± 31.36 and 113.94 ± 39.75 μm, respectively, using the replica technique and 28.97 ± 17.86 and 97.87 ± 21.83 μm, respectively, using OCT. The differences between the techniques were significant (p = 0.00 and p = 0.01, respectively. On evaluation within the groups, internal adaptation values were found to be significantly higher than the marginal adaptation values for the replica technique (p = 0.00 and OCT (p = 0.00. Therefore, the replica and OCT techniques showed different results, with higher values of marginal and internal adaptation found with the replica technique. Marginal and internal adaptation values of ceramic inlays, whether measured by replica or OCT techniques, were within clinically acceptable limits.

  17. From Tls Survey to 3d Solid Modeling for Documentation of Built Heritage: the Case Study of Porta Savonarola in Padua

    Science.gov (United States)

    Guarnieri, A.; Fissore, F.; Masiero, A.; Vettore, A.

    2017-08-01

    It is a matter of fact that 3D visualisation and proper documentation of cultural objects helps to preserve the history and memories of historic buildings, archaeological sites and cultural landscapes, and supports economic growth by stimulating cultural tourism. Preservation, visualisation and recreation of valuable historical and architectural objects and places has always been a serious challenge for specialists in the field. Today, the rapid developments in the fields of close-range photogrammetry, terrestrial laser scanning (TLS) and computer vision (CV) enable to carry out highly accurate 3D models so as to be extremely effective and intuitive for users who have stringent requirements and high expectations. In this note we present the results of the survey and 3D modeling of an ancient gate, Porta Savonarola, located within the remains of the medieval town walls surrounding the historical city center of Padua, Italy. The work has been undertaken within the framework of the project "Walls Multimedia Museum" (WMM) promoted by the local private association "Padua Walls Committee". The goal of the project was to develop a prototype of an "extended" virtual museum, spreaded along most interesting locations of the town walls. The survey of the ancient gate was performed with a Leica C10 and P20 terrestrial laser scanners. Once the acquired scans were properly merged together, a solid model was generated from the global point cloud, and plans and elevations were extracted from it for restoration purposes. A short multimedia video was also created for the "Walls Multimedia Museum", showing both the outer and inner part of the gate. In the paper we will discuss all the steps and challenges addressed to provide the 3D solid model of Porta Savonarola from the TLS data.

  18. [Promoter effect of mercury chloride and methyl-mercury on human keratinocytes in culture].

    Science.gov (United States)

    Zefferino, R; Elia, G; Petrozzi, M T; Leone, A; Corsi, P; Ambrosi, L

    2002-01-01

    Mercury has received considerable media focus because it is present in dental amalgams and seafood. There is potential exposure in gas meters, thermometers and fluorescent lamps workers. To evaluate its possible epigenetic carcinogen effect, cultures of human keratinocytes were treated with increasing doses of HgCl2 for 30 min, 24 h and of CH3HgCl for 24 h, respectively. The red neutral method was used to evaluate the doses of HgCl2 and CH3HgCl which had no cytotoxic effect. Then, the dye transfer method was used to investigate the gap junctions-mediated intercellular communication (GJIC). Cells were microinjected with Lucifer Yellow CH by using the Eppendorf Apparatus and the Leica inverted microscope. After 30 min incubation at the concentration of 10 microM, HgCl2 did not exert inhibition of GJIC. Conversely, after 24 h at the concentration of 10 nM, HgCl2 inhibited GJIC. Incubation with CH3HgCl at the concentration of 250 nM for 24 h reduced the number of fluorescent cells, thus denoting a inhibition of GJIC. Taken together our data demonstrated that: i) HgCl2 and CH3HgCl exerted an inhibitory effect upon GJIC; ii) HgCl2 resulted to inhibit GJIC at concentrations 25 folds lower than CH3HgCl. Further studies will be addressed to evaluate whether the reversal of GJIC inhibition could be obtained by withdrawal of toxic substance, or by the addition of a GJIC activator like the retinoic acid. Finally to shed light on the possible effect of mercury derivates at the transcriptional or translational levels, the expression profile of the connexin 43 gene after HgCl2 and CH3HgCl exposure of cultured human keratinocytes will be investigated.

  19. Aerial Images from AN Uav System: 3d Modeling and Tree Species Classification in a Park Area

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    Gini, R.; Passoni, D.; Pinto, L.; Sona, G.

    2012-07-01

    The use of aerial imagery acquired by Unmanned Aerial Vehicles (UAVs) is scheduled within the FoGLIE project (Fruition of Goods Landscape in Interactive Environment): it starts from the need to enhance the natural, artistic and cultural heritage, to produce a better usability of it by employing audiovisual movable systems of 3D reconstruction and to improve monitoring procedures, by using new media for integrating the fruition phase with the preservation ones. The pilot project focus on a test area, Parco Adda Nord, which encloses various goods' types (small buildings, agricultural fields and different tree species and bushes). Multispectral high resolution images were taken by two digital compact cameras: a Pentax Optio A40 for RGB photos and a Sigma DP1 modified to acquire the NIR band. Then, some tests were performed in order to analyze the UAV images' quality with both photogrammetric and photo-interpretation purposes, to validate the vector-sensor system, the image block geometry and to study the feasibility of tree species classification. Many pre-signalized Control Points were surveyed through GPS to allow accuracy analysis. Aerial Triangulations (ATs) were carried out with photogrammetric commercial software, Leica Photogrammetry Suite (LPS) and PhotoModeler, with manual or automatic selection of Tie Points, to pick out pros and cons of each package in managing non conventional aerial imagery as well as the differences in the modeling approach. Further analysis were done on the differences between the EO parameters and the corresponding data coming from the on board UAV navigation system.

  20. AERIAL IMAGES FROM AN UAV SYSTEM: 3D MODELING AND TREE SPECIES CLASSIFICATION IN A PARK AREA

    Directory of Open Access Journals (Sweden)

    R. Gini

    2012-07-01

    Full Text Available The use of aerial imagery acquired by Unmanned Aerial Vehicles (UAVs is scheduled within the FoGLIE project (Fruition of Goods Landscape in Interactive Environment: it starts from the need to enhance the natural, artistic and cultural heritage, to produce a better usability of it by employing audiovisual movable systems of 3D reconstruction and to improve monitoring procedures, by using new media for integrating the fruition phase with the preservation ones. The pilot project focus on a test area, Parco Adda Nord, which encloses various goods' types (small buildings, agricultural fields and different tree species and bushes. Multispectral high resolution images were taken by two digital compact cameras: a Pentax Optio A40 for RGB photos and a Sigma DP1 modified to acquire the NIR band. Then, some tests were performed in order to analyze the UAV images' quality with both photogrammetric and photo-interpretation purposes, to validate the vector-sensor system, the image block geometry and to study the feasibility of tree species classification. Many pre-signalized Control Points were surveyed through GPS to allow accuracy analysis. Aerial Triangulations (ATs were carried out with photogrammetric commercial software, Leica Photogrammetry Suite (LPS and PhotoModeler, with manual or automatic selection of Tie Points, to pick out pros and cons of each package in managing non conventional aerial imagery as well as the differences in the modeling approach. Further analysis were done on the differences between the EO parameters and the corresponding data coming from the on board UAV navigation system.

  1. Assessment of BRAF V600E Status in Colorectal Carcinoma: Tissue-Specific Discordances between Immunohistochemistry and Sequencing.

    Science.gov (United States)

    Estrella, Jeannelyn S; Tetzlaff, Michael T; Bassett, Roland L; Patel, Keyur P; Williams, Michelle D; Curry, Jonathan L; Rashid, Asif; Hamilton, Stanley R; Broaddus, Russell R

    2015-12-01

    Although sequencing provides the gold standard for identifying colorectal carcinoma with BRAF V600E mutation, immunohistochemistry (IHC) with the recently developed mouse monoclonal antibody VE1 for BRAF V600E protein has shown promise as a more widely available and rapid method. However, we identified anecdotal discordance between VE1 IHC and sequencing results and therefore analyzed VE1 staining by two different IHC methods (Leica Bond and Ventana BenchMark) in whole tissue sections from 480 colorectal carcinomas (323 BRAF wild-type, 142 BRAF V600E mutation, and 15 BRAF non-V600E mutation). We also compared the results with melanomas and papillary thyroid carcinomas (PTC). With the Bond method, among 142 BRAF V600E-mutated colorectal carcinomas, 77 (54%) had diffuse VE1 staining and 48 (33%) had heterogeneous staining, but 17 (12%) were negative. Among 323 BRAF wild-type colorectal carcinomas, 196 (61%) were negative, but 127 (39%) had staining, including 7 with diffuse staining. When positivity was defined as staining in ≥ 20% of tumor cells, VE1 IHC had sensitivity of 75% and specificity of 93% for BRAF V600E mutation. With the Ventana method, among 57 BRAF V600E-mutated colorectal carcinomas, 36 (63%) had diffuse VE1 staining, whereas 6 (11%) had no or weak (colorectal carcinomas, 16 (48%) had no or weak staining, whereas 15 (45%) had heterogeneous staining. In contrast with colorectal carcinoma, Bond and Ventana VE1 IHC in melanoma and PTC were highly concordant with sequencing results. We conclude that VE1 IHC produces suboptimal results in colorectal carcinoma and should not be used to guide patient management. ©2015 American Association for Cancer Research.

  2. Visualization of target inspection data at the National Ignition Facility

    Energy Technology Data Exchange (ETDEWEB)

    Potter, Daniel, E-mail: potter15@llnl.gov [Lawrence Livermore National Laboratory (United States); Antipa, Nick, E-mail: antipa1@llnl.gov [Lawrence Livermore National Laboratory (United States)

    2012-12-15

    Highlights: Black-Right-Pointing-Pointer Target surfaces are measured using a phase-shifting diffraction interferometer. Black-Right-Pointing-Pointer Datasets are several gigabytes that consist of tens to hundreds of files. Black-Right-Pointing-Pointer Software tools that provide a high-level overview of the entire dataset. Black-Right-Pointing-Pointer Single datasets loaded into the visualization session can be individually rotated. Black-Right-Pointing-Pointer Multiple datasets with common features are found then datasets can be aligned. - Abstract: As the National Ignition Facility continues its campaign to achieve ignition, new methods and tools will be required to measure the quality of the target capsules used to achieve this goal. Techniques have been developed to measure capsule surface features using a phase-shifting diffraction interferometer and Leica Microsystems confocal microscope. These instruments produce multi-gigabyte datasets which consist of tens to hundreds of files. Existing software can handle viewing a small subset of an entire dataset, but none can view a dataset in its entirety. Additionally, without an established mode of transport that keeps the target capsules properly aligned throughout the assembly process, a means of aligning the two dataset coordinate systems is needed. The goal of this project is to develop web based software utilizing WebGL which will provide high level overview visualization of an entire dataset, with the capability to retrieve finer details on demand, in addition to facilitating alignment of multiple datasets with one another based on common features that have been visually identified by users of the system.

  3. Shipborne LiDAR system for coastal change monitoring

    Science.gov (United States)

    Kim, chang hwan; Park, chang hong; Kim, hyun wook; hyuck Kim, won; Lee, myoung hoon; Park, hyeon yeong

    2016-04-01

    Coastal areas, used as human utilization areas like leisure space, medical care, ports and power plants, etc., are regions that are continuously changing and interconnected with oceans and land and the sea level has risen by about 8cm (1.9mm / yr) due to global warming from 1964 year to 2006 year in Korea. Coastal erosion due to sea-level rise has caused the problem of marine ecosystems and loss of tourism resources, etc. Regular monitoring of coastal erosion is essential at key locations with such volatility. But the survey method of land mobile LiDAR (light detection and ranging) system has much time consuming and many restrictions. For effective monitoring beach erosion, KIOST (Korea Institute of Ocean Science & Technology) has constructed a shipborne mobile LiDAR system. The shipborne mobile LiDAR system comprised a land mobile LiDAR (RIEGL LMS-420i), an INS (inertial navigation system, MAGUS Inertial+), a RTKGPS (LEICA GS15 GS25), and a fixed platform. The shipborne mobile LiDAR system is much more effective than a land mobile LiDAR system in the measuring of fore shore areas without shadow zone. Because the vessel with the shipborne mobile LiDAR system is continuously moved along the shoreline, it is possible to efficiently survey a large area in a relatively short time. Effective monitoring of the changes using the constructed shipborne mobile LiDAR system for seriously eroded coastal areas will be able to contribute to coastal erosion management and response.

  4. Laser microdissection reveals that transcripts for five plant and one fungal phosphate transporter genes are contemporaneously present in arbusculated cells.

    Science.gov (United States)

    Balestrini, Raffaella; Gómez-Ariza, Jorge; Lanfranco, Luisa; Bonfante, Paola

    2007-09-01

    The establishment of a symbiotic interaction between plant roots and arbuscular mycorrhizal (AM) fungi requires both partners to undergo significant morphological and physiological modifications which eventually lead to reciprocal beneficial effects. Extensive changes in gene expression profiles recently have been described in transcriptomic studies that have analyzed the whole mycorrhizal root. However, because root colonization by AM fungi involves different cell types, a cell-specific gene expression pattern is likely to occur. We have applied the laser microdissection (LMD) technology to investigate expression profiles of both plant and fungal genes in Lycopersicon esculentum roots colonized by Glomus mosseae. A protocol to harvest arbuscule-containing cells from paraffin sections of mycorrhizal roots has been developed using a Leica AS LMD system. RNA of satisfactory quantity and quality has been extracted for molecular analysis. Transcripts for plant phosphate transporters (LePTs), selected as molecular markers for a functional symbiosis, have been detected by reverse-transcriptase polymerase chain reaction assays and associated to distinct cell types, leading to novel insights into the distribution of LePT mRNAs. In fact, the transcripts of the five phosphate transporters (PTs) have been detected contemporaneously in the same arbusculated cell population, unlike from the neighboring noncolonized cells. In addition, fungal H(+)ATPase (GmHA5) and phosphate transporter (GmosPT) mRNAs were found exclusively in arbusculated cells. The discovery that five plant and one fungal PT genes are consistently expressed inside the arbusculated cells provides a new scenario for plant-fungus nutrient exchanges.

  5. Sensitivity and Specificity of Cardiac Tissue Discrimination Using Fiber-Optics Confocal Microscopy.

    Science.gov (United States)

    Huang, Chao; Sachse, Frank B; Hitchcock, Robert W; Kaza, Aditya K

    2016-01-01

    Disturbances of the cardiac conduction system constitute a major risk after surgical repair of complex cases of congenital heart disease. Intraoperative identification of the conduction system may reduce the incidence of these disturbances. We previously developed an approach to identify cardiac tissue types using fiber-optics confocal microscopy and extracellular fluorophores. Here, we applied this approach to investigate sensitivity and specificity of human and automated classification in discriminating images of atrial working myocardium and specialized tissue of the conduction system. Two-dimensional image sequences from atrial working myocardium and nodal tissue of isolated perfused rodent hearts were acquired using a fiber-optics confocal microscope (Leica FCM1000). We compared two methods for local application of extracellular fluorophores: topical via pipette and with a dye carrier. Eight blinded examiners evaluated 162 randomly selected images of atrial working myocardium (n = 81) and nodal tissue (n = 81). In addition, we evaluated the images using automated classification. Blinded examiners achieved a sensitivity and specificity of 99.2 ± 0.3% and 98.0 ± 0.7%, respectively, with the dye carrier method of dye application. Sensitivity and specificity was similar for dye application via a pipette (99.2 ± 0.3% and 94.0 ± 2.4%, respectively). Sensitivity and specificity for automated methods of tissue discrimination were similarly high. Human and automated classification achieved high sensitivity and specificity in discriminating atrial working myocardium and nodal tissue. We suggest that our findings facilitate clinical translation of fiber-optics confocal microscopy as an intraoperative imaging modality to reduce the incidence of conduction disturbances during surgical correction of congenital heart disease.

  6. Contribution of glomerular morphometry to the diagnosis of pediatric nephropathies

    Directory of Open Access Journals (Sweden)

    Mariana Barreto Marini

    2016-01-01

    Full Text Available Only a few studies describe histopathological changes in renal biopsies performed in pediatric patients. This study was conducted to identify an association between morphometric data in renal biopsies and renal function of these patients. Fifty-nine individuals with ages between 2 and 18 years old were selected, who were divided into six groups consisting of frequent nephropathies in children and adolescents and one control group. Proteinuria, urea, and creatinine values of the patients were recorded. Interactive image analysis software Leica QWin[®]was used for morpho- metric analysis of Bowman′s capsule, glomerular capillary tuft, and Bowman′s space area. The mean glomerular tuft area was higher in the membranous glomerulopathy group than in the podo- cytopathy group (57,101 ± 25,094 vs. 27,420 c ± 6279 µm2; P <0.05. The median of Bowman′s space area was higher in the control group than in the podocytopathy group and in the thin basement membrane/Alport syndrome group [12,210 (7676-26,945 vs. 5801 (3031-7852 µm2; P <0.01 and 12210 (7676-26,945 vs. 4183 (3797-7992 µm2; P <0.01, respectively]. There was a positive and significant correlation between Bowman′s capsule area and the levels of proteinuria, creatinine, and urea of the patients, as well as between the glomerular tuft area and the levels of proteinuria, creatinine, and urea in the patients, regardless of their nephropathy. Glomerular morphometry may contribute to the diagnosis of some glomerulopathies and the association between glomerular morphometric parameters, and laboratory data may promote a better understanding of the prognosis of these patients.

  7. Changes in rat testis morphology and androgen receptor expression around the age of puberty.

    Science.gov (United States)

    Abd El-Meseeh, Nabila A; El-Shaarawy, Ehab A A; AlDomairy, Ahmed F; Sehly, Reem A Abou

    2016-05-01

    Androgens are the keystone in fertility and intact sexual functions in males. It exerts its actions via androgen receptors extensively present in testicular cells, only its presence in germ cells is controversial. The alteration of androgen receptors in different testicular cells is usually accompanied by different sexual disorders. On the other hand, many sexual disorders are treated with androgens. Puberty, being the juncture of hormonal blossom, is an important stage to evaluate the evolution of testicular cells including androgen receptors. The aim of the work was to investigate the morphological and androgen receptor changes in different testicular cells during puberty in the rat testis using histological and immunohistochemical techniques. This study was carried out on 45 male albino rats (Sprague-Dawley). The rats were divided into three age groups; group I (prepubertal) 21 days old, group II (peripubertal) 35 days and group III (postpubertal) 90 days old. The rat testes were examined histologically and immuneohistochemically. Cells and androgen receptors were counted using Leica Qwin 500 image analyzer computer system. Data were analyzed using univariate ANOVA and Bonferroni post hoc test. Histological examination of the different ages showed developmental changes of different testicular cells. Immunohistochemical examination revealed the presence of AR in spermatogenic cells in pubertal and postpubertal groups and partially in prepubertal group. AR was clearly expressed in both Sertoli and Leydig cells in the three groups. The maximum expression in Sertoli cells was at 90 days while that of Leydig cells was at the age of 35 days. Androgen receptors should not be excluded as an effective factor on germ cells through its direct action on AR, clearly expressed in spermatogenic cells and its surge at the age of puberty. Studies and treatments should respect the AR expected levels according to age in other testicular cells as well. Sertoli cells show a linear

  8. Topographical survey work and stake out of an agroindustrial building from Periam village, Timis county

    Directory of Open Access Journals (Sweden)

    Livia Luminita Barliba

    2016-11-01

    Full Text Available The main objective of the project was to achieve a topographic survey works and mapping of an agro-purpose storage for vegetables and fruits produced by the existing farms from the village. The work theme includes achieving of cadastral plans base on a 1/5000 scale, using  measurements for all studied locations and land cadastre units. GPS technology used in thickening RGNS, namely the determination of network support, lead to the lifting of all topographical stake out of  the contour points. (Bârliba Luminiţa Livia, et. all.,2004, After that, the points were used as starting points for execution the tracing of the surface construction with the support of a total station. For topographical survey was used a V82 South GNSS receiver with two frequencies and 7 channels which allows RTK positioning in real time and then it was used  permanent topographic station nearby Timisoara (TIM1.2.3. After overlapping the situation plan and  plan developed by an architect, it was made the stake out of industrial building’s foundation with Leica Total Station 1205+, through the dedicated "Stakeout" software. Topographical works generally pursue two base objectives: the topographical survey completed by a digital representation of a small land area and staking out the construction building  respectively implementing the projects on the ground (Bârliba Luminiţa Livia, et. all.,2005. This paper demonstrated that it is possible to achieve the optimal time of all field and office operations with precision and accuracy parameters required by the topographical laws and rules.

  9. Chalcedony (a crystalline variety of silica): biogenic origin in electric organs from living Psammobatis extenta (family Rajidae).

    Science.gov (United States)

    Prado Figueroa, María; Barrera, Facundo; Cesaretti, Nora N

    2008-10-01

    The electric organs of electric fish have been used extensively for the study of peripheral cholinergic synapses. Aluminum and silicon have been observed in the electrocytes of Psammobatis extenta, a fish belonging to the family Rajidae, using a combination of scanning electron microscopy and X-ray spectrometry. Based on this evidence, the presence of silica minerals has been documented by means of mineralogical techniques. Electric organ cryostat sections and subcellular fractions were observed using a Leica DMLP mineralogical microscope. The shape, size and color, among other properties, were analyzed in plane-polarized light, while birefringence and the extinction angle, which allow for mineral identification, were observed through crossed-polarized illumination. The distribution of chalcedony, an oxide silicon mineral, in the sections and all the fractions of the electric organ was recorded. X-ray diffraction analysis of the electric organ segments showed a similar result, with a low-quartz variety. Chalcedony precipitation occurred at a specific pH (7-8) and oxidation potential (Eh; 0.0 to -0.2). This observation supports the important role played by pH and Eh conditions in silica precipitation in electrocytes, as has been reported in geological environments. It is possible that silica formation and silica degradation in electric organs are also related to the enzymes, silicatein and silicase, that direct the polymerization and depolymerization of amorphous silica in sponges. Carbonic anhydrases (silicase) are involved in physiological pH regulation. Crystallization of chalcedony via spiral growth from a partially polymerized fluid is consistent with processes known to occur in organic systems. This is the first time that a biogenically produced crystalline mineral phase (i.e., chalcedony) has been observed in the electrocytes and cholinergic nerves from living electric fish.

  10. Biosilicification (chalcedony) in human cerebral cortex, hippocampus and cerebellum from aged patients.

    Science.gov (United States)

    Prado Figueroa, María; Flores, Luis; Sanchez, Juvenal; Cesaretti, Nora

    2008-10-01

    Aluminum and silicon have been observed to be present in the human degenerated brain and normal elderly brains by using a combination of scanning electron microscopy and X-ray spectrometry (EDS-SEM). Al and Si of electric organs were also reported--in electrocytes and cholinergic nerves--from living electric fish (family Rajidae). A biogenically produced crystalline mineral phase (i.e., chalcedony) has also been observed in electric organs by using a mineralogical microscope. Based on this evidence we decided to explore the presence of chalcedony (SiO2) in the human central nervous system (CNS). Sections from aged patients (mean, 81 years) were collected after autopsy and observed using a Leica DMLP mineralogical microscope. Chalcedony was detected in cerebral cortex and cerebellum. In plane-polarized light, chalcedony is rounded in shape, 12-20 microm in size, translucent, with a low refraction index. The crossed-polarizer image shows first order birefringence color (grey-white) and radial extinction. Chalcedony was also detected in the hippocampus in large amounts and sizes (50-60 microm). Chalcedony is a microcrystalline fibrous form of silica. It consists of nanoscale intergrowths of quartz and the optically length-slow fibrous silica polymorph moganite. Chalcedony precipitation occurs at a specific pH (7-8) and oxidation potential (Eh; 0.0 to -0.2) in geological environments. This observation supports the important role played by pH and Eh conditions in silica precipitation in elderly brains, as has also been reported in peripheral cholinergic nerves in electric organ from living electric fish. Carbonic anhydrases (CAs) (silicase) are involved in physiological pH regulation and may also be participating in the polymerization-depolymerization of chalcedony in the human brain. This is the first time a biogenically produced crystalline mineral phase (i.e., chalcedony) has been observed in the human CNS from aged patients.

  11. FROM TLS SURVEY TO 3D SOLID MODELING FOR DOCUMENTATION OF BUILT HERITAGE: THE CASE STUDY OF PORTA SAVONAROLA IN PADUA

    Directory of Open Access Journals (Sweden)

    A. Guarnieri

    2017-08-01

    Full Text Available It is a matter of fact that 3D visualisation and proper documentation of cultural objects helps to preserve the history and memories of historic buildings, archaeological sites and cultural landscapes, and supports economic growth by stimulating cultural tourism. Preservation, visualisation and recreation of valuable historical and architectural objects and places has always been a serious challenge for specialists in the field. Today, the rapid developments in the fields of close-range photogrammetry, terrestrial laser scanning (TLS and computer vision (CV enable to carry out highly accurate 3D models so as to be extremely effective and intuitive for users who have stringent requirements and high expectations. In this note we present the results of the survey and 3D modeling of an ancient gate, Porta Savonarola, located within the remains of the medieval town walls surrounding the historical city center of Padua, Italy. The work has been undertaken within the framework of the project “Walls Multimedia Museum” (WMM promoted by the local private association “Padua Walls Committee”. The goal of the project was to develop a prototype of an “extended” virtual museum, spreaded along most interesting locations of the town walls. The survey of the ancient gate was performed with a Leica C10 and P20 terrestrial laser scanners. Once the acquired scans were properly merged together, a solid model was generated from the global point cloud, and plans and elevations were extracted from it for restoration purposes. A short multimedia video was also created for the “Walls Multimedia Museum”, showing both the outer and inner part of the gate. In the paper we will discuss all the steps and challenges addressed to provide the 3D solid model of Porta Savonarola from the TLS data.

  12. Morphological Changes Along a Dike Landside Slope Sampled by 4d High Resolution Terrestrial Laser Scanning

    Science.gov (United States)

    Herrero-Huertaa, Mónica; Lindenbergh, Roderik; Ponsioen, Luc; van Damme, Myron

    2016-06-01

    Emergence of light detection and ranging (LiDAR) technology provides new tools for geomorphologic studies improving spatial and temporal resolution of data sampling hydrogeological instability phenomena. Specifically, terrestrial laser scanning (TLS) collects high resolution 3D point clouds allowing more accurate monitoring of erosion rates and processes, and thus, quantify the geomorphologic change on vertical landforms like dike landside slopes. Even so, TLS captures observations rapidly and automatically but unselectively. In this research, we demonstrate the potential of TLS for morphological change detection, profile creation and time series analysis in an emergency simulation for characterizing and monitoring slope movements in a dike. The experiment was performed near Schellebelle (Belgium) in November 2015, using a Leica Scan Station C10. Wave overtopping and overflow over a dike were simulated whereby the loading conditions were incrementally increased and 14 successful scans were performed. The aim of the present study is to analyse short-term morphological dynamic processes and the spatial distribution of erosion and deposition areas along a dike landside slope. As a result, we are able to quantify the eroded material coming from the impact on the terrain induced by wave overtopping which caused the dike failure in a few minutes in normal storm scenarios (Q = 25 l/s/m) as 1.24 m3. As this shows that the amount of erosion is measurable using close range techniques; the amount and rate of erosion could be monitored to predict dike collapse in emergency situation. The results confirm the feasibility of the proposed methodology, providing scalability to a comprehensive analysis over a large extension of a dike (tens of meters).

  13. MORPHOLOGICAL CHANGES ALONG A DIKE LANDSIDE SLOPE SAMPLED BY 4D HIGH RESOLUTION TERRESTRIAL LASER SCANNING

    Directory of Open Access Journals (Sweden)

    M. Herrero-Huertaa

    2016-06-01

    Full Text Available Emergence of light detection and ranging (LiDAR technology provides new tools for geomorphologic studies improving spatial and temporal resolution of data sampling hydrogeological instability phenomena. Specifically, terrestrial laser scanning (TLS collects high resolution 3D point clouds allowing more accurate monitoring of erosion rates and processes, and thus, quantify the geomorphologic change on vertical landforms like dike landside slopes. Even so, TLS captures observations rapidly and automatically but unselectively. In this research, we demonstrate the potential of TLS for morphological change detection, profile creation and time series analysis in an emergency simulation for characterizing and monitoring slope movements in a dike. The experiment was performed near Schellebelle (Belgium in November 2015, using a Leica Scan Station C10. Wave overtopping and overflow over a dike were simulated whereby the loading conditions were incrementally increased and 14 successful scans were performed. The aim of the present study is to analyse short-term morphological dynamic processes and the spatial distribution of erosion and deposition areas along a dike landside slope. As a result, we are able to quantify the eroded material coming from the impact on the terrain induced by wave overtopping which caused the dike failure in a few minutes in normal storm scenarios (Q = 25 l/s/m as 1.24 m3. As this shows that the amount of erosion is measurable using close range techniques; the amount and rate of erosion could be monitored to predict dike collapse in emergency situation. The results confirm the feasibility of the proposed methodology, providing scalability to a comprehensive analysis over a large extension of a dike (tens of meters.

  14. Laser Tracker Utilization Methodology in Measuring Truth Trajectories for INS Testing on 6 Degree of Freedom Table at the Marshall Space Flight Center's Contact Dynamics Simulation Laboratory with Lessons Learned

    Science.gov (United States)

    Leggett, Jared O.; Bryant, Thomas C.; Cowen, Charles T.; Clifton, Billy W.

    2018-01-01

    When performing Inertial Navigation System (INS) testing at the Marshall Space Flight Center's (MSFC) Contact Dynamics Simulation Laboratory (CDSL) early in 2017, a Leica Geosystems AT901 Laser Tracker system (LLT) measured the twist & sway trajectories as generated by the 6 Degree Of Freedom (6DOF) Table in the CDSL. These LLT measured trajectories were used in the INS software model validation effort. Several challenges were identified and overcome during the preparation for the INS testing, as well as numerous lessons learned. These challenges included determining the position and attitude of the LLT with respect to an INS-shared coordinate frame using surveyed monument locations in the CDSL and the accompanying mathematical transformation, accurately measuring the spatial relationship between the INS and a 6DOF tracking probe due to lack of INS visibility from the LLT location, obtaining the data from the LLT during a test, determining how to process the results for comparison with INS data in time and frequency domains, and using a sensitivity analysis of the results to verify the quality of the results. While many of these challenges were identified and overcome before or during testing, a significant lesson on test set-up was not learned until later in the data analysis process. It was found that a combination of trajectory-dependent gimbal locking and environmental noise introduced non-negligible noise in the angular measurements of the LLT that spanned the evaluated frequency spectrum. The lessons learned in this experiment may be useful for others performing INS testing in similar testing facilities.

  15. Radiometric Normalization of Large Airborne Image Data Sets Acquired by Different Sensor Types

    Science.gov (United States)

    Gehrke, S.; Beshah, B. T.

    2016-06-01

    Generating seamless mosaics of aerial images is a particularly challenging task when the mosaic comprises a large number of im-ages, collected over longer periods of time and with different sensors under varying imaging conditions. Such large mosaics typically consist of very heterogeneous image data, both spatially (different terrain types and atmosphere) and temporally (unstable atmo-spheric properties and even changes in land coverage). We present a new radiometric normalization or, respectively, radiometric aerial triangulation approach that takes advantage of our knowledge about each sensor's properties. The current implementation supports medium and large format airborne imaging sensors of the Leica Geosystems family, namely the ADS line-scanner as well as DMC and RCD frame sensors. A hierarchical modelling - with parameters for the overall mosaic, the sensor type, different flight sessions, strips and individual images - allows for adaptation to each sensor's geometric and radiometric properties. Additional parameters at different hierarchy levels can compensate radiome-tric differences of various origins to compensate for shortcomings of the preceding radiometric sensor calibration as well as BRDF and atmospheric corrections. The final, relative normalization is based on radiometric tie points in overlapping images, absolute radiometric control points and image statistics. It is computed in a global least squares adjustment for the entire mosaic by altering each image's histogram using a location-dependent mathematical model. This model involves contrast and brightness corrections at radiometric fix points with bilinear interpolation for corrections in-between. The distribution of the radiometry fixes is adaptive to each image and generally increases with image size, hence enabling optimal local adaptation even for very long image strips as typi-cally captured by a line-scanner sensor. The normalization approach is implemented in HxMap software. It has been

  16. Avaliação de uma técnica para geração de modelos digitais de superfície utilizando múltiplas imagens

    Directory of Open Access Journals (Sweden)

    Raquel Alves de Oliveira

    Full Text Available A utilização de imagens ópticas para a geração de Modelos Digitais de Superfície (MDSs é explorada há muitos anos e os resultados dependem, além das características do projeto (resolução das imagens, tamanho da área de sobreposição entre as imagens, etc, das técnicas de correspondência de imagens aplicadas e da capacidade computacional para o processamento das imagens. Os pontos gerados com erros no processo de correspondência influenciam diretamente a qualidade dos produtos obtidos e, consequentemente, aumentam a necessidade da etapa onerosa de edição. O objetivo deste trabalho é a avaliação da técnica de geração de MDSs utilizando a correspondência simultânea entre múltiplas imagens pelo método de busca em linhas verticais (Vertical Line Locus-VLL. Nos experimentos foram utilizadas seis imagens da área urbana de Presidente Prudente-SP, com elemento de resolução aproximado de 7cm. Para análise da qualidade foram gerados MDSs de uma pequena área contendo áreas homogêneas, padrão repetitivo, objetos em movimento incluindo sombras e árvores grandes. O MDS obtido foi comparado com dados de varredura a LASER (Light Amplification by Simulated Emission of Radiation e com o MDS gerado pelo software Leica Photogrammetric Suite (LPS. Os resultados mostraram a compatibilidade da qualidade entre os modelos gerados pela técnica implementada e os dados de referência.

  17. Urban planning and agriculture. Methodology for assessing rooftop greenhouse potential of non-residential areas using airborne sensors.

    Science.gov (United States)

    Nadal, Ana; Alamús, Ramón; Pipia, Luca; Ruiz, Antonio; Corbera, Jordi; Cuerva, Eva; Rieradevall, Joan; Josa, Alejandro

    2017-12-01

    The integration of rooftop greenhouses (RTGs) in urban buildings is a practice that is becoming increasingly important in the world for their contribution to food security and sustainable development. However, the supply of tools and procedures to facilitate their implementation at the city scale is limited and laborious. This work aims to develop a specific and automated methodology for identifying the feasibility of implementation of rooftop greenhouses in non-residential urban areas, using airborne sensors. The use of Light Detection and Ranging (LIDAR) and Long Wave Infrared (LWIR) data and the Leica ALS50-II and TASI-600 sensors allow for the identification of some building roof parameters (area, slope, materials, and solar radiation) to determine the potential for constructing a RTG. This development represents an improvement in time and accuracy with respect to previous methodology, where all the relevant information must be acquired manually. The methodology has been applied and validated in a case study corresponding to a non-residential urban area in the industrial municipality of Rubí, Barcelona (Spain). Based on this practical application, an area of 36,312m 2 out of a total area of 1,243,540m 2 of roofs with ideal characteristics for the construction of RTGs was identified. This area can produce approximately 600tons of tomatoes per year, which represents the average yearly consumption for about 50% of Rubí total population. The use of this methodology also facilitates the decision making process in urban agriculture, allowing a quick identification of optimal surfaces for the future implementation of urban agriculture in housing. It also opens new avenues for the use of airborne technology in environmental topics in cities. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. ACCURACY ASSESSMENT OF CROWN DELINEATION METHODS FOR THE INDIVIDUAL TREES USING LIDAR DATA

    Directory of Open Access Journals (Sweden)

    K. T. Chang

    2016-06-01

    Full Text Available Forest canopy density and height are used as variables in a number of environmental applications, including the estimation of biomass, forest extent and condition, and biodiversity. The airborne Light Detection and Ranging (LiDAR is very useful to estimate forest canopy parameters according to the generated canopy height models (CHMs. The purpose of this work is to introduce an algorithm to delineate crown parameters, e.g. tree height and crown radii based on the generated rasterized CHMs. And accuracy assessment for the extraction of volumetric parameters of a single tree is also performed via manual measurement using corresponding aerial photo pairs. A LiDAR dataset of a golf course acquired by Leica ALS70-HP is used in this study. Two algorithms, i.e. a traditional one with the subtraction of a digital elevation model (DEM from a digital surface model (DSM, and a pit-free approach are conducted to generate the CHMs firstly. Then two algorithms, a multilevel morphological active-contour (MMAC and a variable window filter (VWF, are implemented and used in this study for individual tree delineation. Finally, experimental results of two automatic estimation methods for individual trees can be evaluated with manually measured stand-level parameters, i.e. tree height and crown diameter. The resulting CHM generated by a simple subtraction is full of empty pixels (called "pits" that will give vital impact on subsequent analysis for individual tree delineation. The experimental results indicated that if more individual trees can be extracted, tree crown shape will became more completely in the CHM data after the pit-free process.

  19. The DISCOVER Study 3-Year Results: Feasibility and Usefulness of Microscope-Integrated Intraoperative OCT during Ophthalmic Surgery.

    Science.gov (United States)

    Ehlers, Justis P; Modi, Yasha S; Pecen, Paula E; Goshe, Jeff; Dupps, William J; Rachitskaya, Aleksandra; Sharma, Sumit; Yuan, Alex; Singh, Rishi; Kaiser, Peter K; Reese, Jamie L; Calabrise, Carmen; Watts, Allison; Srivastava, Sunil K

    2018-02-03

    To report the 3-year assessment of feasibility and usefulness of microscope-integrated intraoperative OCT (iOCT) during ophthalmic surgery. Prospective, consecutive case series. Adult participants undergoing incisional ophthalmic surgery with iOCT imaging who consented to be enrolled in the Determination of Feasibility of Intraoperative Spectral-Domain Microscope Combined/Integrated OCT Visualization during En Face Retinal and Ophthalmic Surgery (DISCOVER) study. The DISCOVER study is a single-site, multisurgeon, institutional review board-approved investigational device prospective study. Participants included patients undergoing anterior or posterior segment surgery who underwent iOCT imaging with 1 of 3 prototype microscope-integrated iOCT systems (i.e., Zeiss Rescan 700, Leica EnFocus, or Cole Eye iOCT systems). Clinical characteristics were documented, iOCT was directed by the operating surgeon at predetermined surgical time points, and each surgeon completed a questionnaire after surgery to evaluate the usefulness of iOCT during surgery. Feasibility of iOCT based ability to obtain an OCT image during surgery and usefulness of iOCT based on surgeon reporting during surgery. Eight hundred thirty-seven eyes (244 anterior segment cases and 593 posterior segment cases) were enrolled in the DISCOVER study. Intraoperative OCT demonstrated feasibility with successful image acquisition in 820 eyes (98.0%; 95% confidence interval [CI], 96.8%-98.8%). In 106 anterior segment cases (43.4%; 95% CI, 37.1%-49.9%), the surgeons indicated that the iOCT information impacted their surgical decision making and altered the procedure. In posterior segment procedures, surgeons reported that iOCT enabled altered surgical decision making during the procedure in 173 cases (29.2%; 95% CI, 25.5%-33.0%). The DISCOVER iOCT study demonstrated both generalized feasibility and usefulness based on the surgeon-reported impact on surgical decision making. This large-scale study confirmed similar

  20. High-resolution Digital Mapping of Historical Lava Flows as a Test-bed for Lava Flow Models

    Science.gov (United States)

    Pyle, D. M.; Parks, M.; Nomikou, P.; Mather, T. A.; Simou, E.; Kalnins, L. M.; Paulatto, M.; Watts, A. B.

    2013-12-01

    Quantitative analysis of high-resolution lava flow morphology can improve our understanding of past effusive eruptions by providing insight into eruptive processes and the rheological properties of erupted magmas. We report the results of an ongoing investigation into the young dacite lava flows of the Kameni islands, Santorini volcano, Greece, which were emplaced during both subaerial and shallow submarine eruptions over the past 3000 years. Historical eruptions of the Kameni islands since 1866 have been very carefully documented in contemporaneous scientific reports. Eruptions since 1573 appear to be time-predictable, with a close relationship between eruption length, the size of extruded lava domes, and the time elapsed since the previous eruption. A new NERC - Airborne Survey and Research Facility LiDAR survey of the Kameni islands was completed in May 2012, using a Leica ALS50 Airborne Laser Scanner mounted on a Dornier 228 aircraft. The topographic surface was mapped at an average point density of 2.1 points per square metre, and covers the entire extent of the youngest subaerial lava flow fields on Santorini. A 2-m DEM derived from the 2012 LiDAR dataset was merged with a 5-m resolution bathymetric grid, based on multibeam surveys carried out by the Hellenic Centre for Marine Research, during cruises in 2001 and 2006, using a SEABEAM 2120 hull-mounted swath system. The resultant grid provides the first high resolution map of both subaerial and submarine historic lava flows emplaced in the centre of the Santorini caldera, and includes several previously unidentified submarine flows and cones. Attribute maps were used to delineate and identify discrete lava flows both onshore and offshore; and morphometric profiles were used to compute accurate volumetric estimates for each of the historic flows, and to determine bulk rheological properties of the lavas, assuming a Bingham rheology. This ongoing work will improve our analysis of the relationship between

  1. Apoyo a Estudios Geodinamicos con GPS en Guatemala

    Science.gov (United States)

    Robles, V. R.

    2013-05-01

    El Instituto Geografico Nacional de Guatemala implemento 17 estaciones GNSS en el año 2009, como un proyecto de credito mixto de donacion de equipamiento del Gobierno de Suiza, el cual, este equipamiento de estaciones CORS GNSS es un sistema de recepción y transmisión de datos crudos GPS RInex que utiliza la tecnologia Spider Web de Leica, asi mismo este sistema esta sirviendo para el espablecimiento de un marco geodesico nacional de coordenadas geodesicas oficiales, el cual se calculan u obtienen las velocidades en tiempos temporales programados de las 17 Estaciones CORS. La infraestructura del marco geodesico de Guatemala esta sirviendo de base para las aplicaciones de estudios geodinamicos como el monitoreo de del desplazamiento de las placas tectonicas por medio de un estudio que se inicio en el año de 1999, llamado medicion con GPS el sistema de Fallas de los rios Polochic Motagua de Guatemala, tambien para un estudio que se implemento para deformación de corteza terrestre local en un Volcan Activo de Guatemala llamado Pacaya. Para el estudio de medicion con GPS en el sistema de falla de los Rios del polochic Motagua se implementaron 16 puntos para medir con GPS de dos frecuencias en el año de 1999, el cual, tres puntos son estaciones geodesicas CORS IGS llamados GUAT, ELEN y HUEH, despues en el año de 2003 se hizo otra medicion en un total de 20 puntos, que permitió calcular las velocidades de desplazamieinto de los puntos en mención, usando como referencia el modelo NUVEL 1A de DeMets de la placa de Norteamerica. Este estudio fue en cooperación internacional por la universidad de Nice de Francia y el IGNde Francia. Para el estudio del monitoreo con GPS del volcan activo de Guatemala, se implementaron cuatro puntos al rededor del volcan, el cual, se realizan cuatro mediciones al año, que permiten determinar axialmente la distancias entre los puntos, y rebisar estadisticamente cual es el comportamiento de las distancias en funcion del tiempo, si

  2. DEM time series of an agricultural watershed

    Science.gov (United States)

    Pineux, Nathalie; Lisein, Jonathan; Swerts, Gilles; Degré, Aurore

    2014-05-01

    In agricultural landscape soil surface evolves notably due to erosion and deposition phenomenon. Even if most of the field data come from plot scale studies, the watershed scale seems to be more appropriate to understand them. Currently, small unmanned aircraft systems and images treatments are improving. In this way, 3D models are built from multiple covering shots. When techniques for large areas would be to expensive for a watershed level study or techniques for small areas would be too time consumer, the unmanned aerial system seems to be a promising solution to quantify the erosion and deposition patterns. The increasing technical improvements in this growth field allow us to obtain a really good quality of data and a very high spatial resolution with a high Z accuracy. In the center of Belgium, we equipped an agricultural watershed of 124 ha. For three years (2011-2013), we have been monitoring weather (including rainfall erosivity using a spectropluviograph), discharge at three different locations, sediment in runoff water, and watershed microtopography through unmanned airborne imagery (Gatewing X100). We also collected all available historical data to try to capture the "long-term" changes in watershed morphology during the last decades: old topography maps, soil historical descriptions, etc. An erosion model (LANDSOIL) is also used to assess the evolution of the relief. Short-term evolution of the surface are now observed through flights done at 200m height. The pictures are taken with a side overlap equal to 80%. To precisely georeference the DEM produced, ground control points are placed on the study site and surveyed using a Leica GPS1200 (accuracy of 1cm for x and y coordinates and 1.5cm for the z coordinate). Flights are done each year in December to have an as bare as possible ground surface. Specific treatments are developed to counteract vegetation effect because it is know as key sources of error in the DEM produced by small unmanned aircraft

  3. Avulsion research using flume experiments and highly accurate and temporal-rich SfM datasets

    Science.gov (United States)

    Javernick, L.; Bertoldi, W.; Vitti, A.

    2017-12-01

    SfM's ability to produce high-quality, large-scale digital elevation models (DEMs) of complicated and rapidly evolving systems has made it a valuable technique for low-budget researchers and practitioners. While SfM has provided valuable datasets that capture single-flood event DEMs, there is an increasing scientific need to capture higher temporal resolution datasets that can quantify the evolutionary processes instead of pre- and post-flood snapshots. However, flood events' dangerous field conditions and image matching challenges (e.g. wind, rain) prevent quality SfM-image acquisition. Conversely, flume experiments offer opportunities to document flood events, but achieving consistent and accurate DEMs to detect subtle changes in dry and inundated areas remains a challenge for SfM (e.g. parabolic error signatures).This research aimed at investigating the impact of naturally occurring and manipulated avulsions on braided river morphology and on the encroachment of floodplain vegetation, using laboratory experiments. This required DEMs with millimeter accuracy and precision and at a temporal resolution to capture the processes. SfM was chosen as it offered the most practical method. Through redundant local network design and a meticulous ground control point (GCP) survey with a Leica Total Station in red laser configuration (reported 2 mm accuracy), the SfM residual errors compared to separate ground truthing data produced mean errors of 1.5 mm (accuracy) and standard deviations of 1.4 mm (precision) without parabolic error signatures. Lighting conditions in the flume were limited to uniform, oblique, and filtered LED strips, which removed glint and thus improved bed elevation mean errors to 4 mm, but errors were further reduced by means of an open source software for refraction correction. The obtained datasets have provided the ability to quantify how small flood events with avulsion can have similar morphologic and vegetation impacts as large flood events

  4. Accuracy Assessment of a Complex Building 3d Model Reconstructed from Images Acquired with a Low-Cost Uas

    Science.gov (United States)

    Oniga, E.; Chirilă, C.; Stătescu, F.

    2017-02-01

    Nowadays, Unmanned Aerial Systems (UASs) are a wide used technique for acquisition in order to create buildings 3D models, providing the acquisition of a high number of images at very high resolution or video sequences, in a very short time. Since low-cost UASs are preferred, the accuracy of a building 3D model created using this platforms must be evaluated. To achieve results, the dean's office building from the Faculty of "Hydrotechnical Engineering, Geodesy and Environmental Engineering" of Iasi, Romania, has been chosen, which is a complex shape building with the roof formed of two hyperbolic paraboloids. Seven points were placed on the ground around the building, three of them being used as GCPs, while the remaining four as Check points (CPs) for accuracy assessment. Additionally, the coordinates of 10 natural CPs representing the building characteristic points were measured with a Leica TCR 405 total station. The building 3D model was created as a point cloud which was automatically generated based on digital images acquired with the low-cost UASs, using the image matching algorithm and different software like 3DF Zephyr, Visual SfM, PhotoModeler Scanner and Drone2Map for ArcGIS. Except for the PhotoModeler Scanner software, the interior and exterior orientation parameters were determined simultaneously by solving a self-calibrating bundle adjustment. Based on the UAS point clouds, automatically generated by using the above mentioned software and GNSS data respectively, the parameters of the east side hyperbolic paraboloid were calculated using the least squares method and a statistical blunder detection. Then, in order to assess the accuracy of the building 3D model, several comparisons were made for the facades and the roof with reference data, considered with minimum errors: TLS mesh for the facades and GNSS mesh for the roof. Finally, the front facade of the building was created in 3D based on its characteristic points using the PhotoModeler Scanner

  5. Internal adaptation of cast titanium crowns

    Directory of Open Access Journals (Sweden)

    Sicknan Soares da Rocha

    2007-08-01

    Full Text Available As the adaptation of titanium crowns obtained by Rematitan Plus investment, specific for titanium, is not recognized to be suitable, this study evaluated the effect of the concentration of the specific liquid and the temperature of the mold of investments on the internal misfit of crowns cast on commercially pure titanium. Individual dies of epoxy resin were obtained, representing teeth prepared for full-crown restoration with a 6-degree axial surface convergence angle and shoulder (1.0 mm. For the waxing of each crown, a ring-shaped stainless steel matrix (8.0mm internal diameter; 7.5 mm height was adapted above the individual dies of epoxy resin. The Rematian Plus investment was mixed according to the manufacturer's instructions using two different concentrations of the specific liquid: 100%, 75%. Casting was performed in a Discovery Plasma Ar-arc vacuum-pressure casting machine with molds at temperatures of 430ºC, 515ºC and 600ºC. The crowns were cleaned individually in a solution (1% HF + 13% HNO3 for 10 min using a ultrasonic cleaner, with no internal adaptations, and luted with zinc phosphate cement under a 5 kg static load. The crown and die assemblies were embedded in resin and sectioned longitudinally. The area occupied by cement was observed using stereoscopic lens (10X and measured by the Leica Qwin image analysis system (mm². The data for each experimental condition (n=8 were analyzed by Kruskal-Wallis non-parametric test (á=0.05. The results showed that liquid dilution and the increase in mold temperature did not significantly influence the levels of internal fit of the cast titanium crowns. The lowest means (±SD of internal misfit were obtained for the 430ºC/100%: (7.25 mm² ±1.59 and 600ºC/100% (8.8 mm² ±2.25 groups, which presented statistically similar levels of internal misfit.

  6. GATA 3 – A MULTISPECIFIC BUT POTENTIALLY USEFUL MARKER IN SURGICAL PATHOLOGY – A SYSTEMATIC ANALYSIS OF 2500 EPITHELIAL AND NON-EPITHELIAL TUMORS

    Science.gov (United States)

    Miettinen, Markku; Cue, Peter A. Mc.; Sarlomo-Rikala, Maarit; Rys, Janusz; Czapiewski, Piotr; Wazny, Krzysztof; Langfort, Renata; Waloszczyk, Piotr; Biernat, Wojciech; Lasota, Jerzy; Wang, Zengfeng

    2013-01-01

    GATA3 is a transcription factor important in the differentiation of breast epithelia, urothelia, and subsets of T-lymphocytes. It has been suggested useful in the evaluation of mammary or urothelial origin or metastatic carcinomas, but its distribution in normal and neoplastic tissues is incompletely mapped. In this study, we examined normal developing and adult tissues in 2040 epithelial and 460 mesenchymal or neuroectodermal neoplasms for GATA3 expression to explore its diagnostic value in surgical pathology, using monoclonal antibody (clone L50-823) and Leica Bond automated immunohistochemistry. GATA3 was expressed in trophoblast, fetal and adult epidermis, adult mammary and some salivary gland and sweat gland ductal epithelia, urothelia, distal nephron in developing and adult tissues, some prostatic basal cells, and subsets of T-lymphocytes. It was expressed stronger in fetal than adult mesothelia and was absent in respiratory and gastrointestinal epithelia. In epithelial neoplasms, GATA3 was expressed in >90% of primary and metastatic ductal and lobular carcinomas of the breast, urothelial, and cutaneous basal cell carcinomas, and trophoblastic and endodermal sinus tumors. In metastatic breast carcinomas, it was more sensitive than GCDFP. Among squamous cell carcinomas, the expression was highest in the skin (81%) and lower in cervical (33%), laryngeal (16%) and pulmonary tumors (12%). Common positivity was found in skin adnexal tumors (100%), mesothelioma (58%), salivary gland (43%) and pancreatic (37%) ductal carcinomas, whereas frequency of expression in adenocarcinomas of lung, stomach, colon, endometrium, ovary, and prostate was tumor with frequent positivity (51%), whereas oncocytomas were positive in 17% of cases but other types only rarely. Among mesenchymal and neuroectodermal tumors, paragangliomas were usually positive, which sets these tumors apart from epithelial neuroendocrine tumors. Mesenchymal tumors were only sporadically positive, except

  7. Distribuição dos depósitos de glicogênio durante a odontogênese intra-uterina em Calomys callosus = Distribution of glycogen storage during odontogenesis in fetal development in Calomys callosus

    Directory of Open Access Journals (Sweden)

    Vieira, Andreia Espíndola

    2006-01-01

    Full Text Available As células animais estocam carboidratos na forma de glicogênio como fonte de energia. Esses depósitos ocorrem amplamente nos tecidos embrionários para disponibilização rápida de energia, principalmente durante a diferenciação e o desenvolvimento das células. O presente trabalho avaliou a distribuição de depósitos de glicogênio durante a odontogênese intra-uterina em Calomys callosus utilizando fetos com 12 a 20 dias, sendo 5 fetos de cada dia. As cabeças dos fetos foram removidas e fixadas em formaldeído 10% em PBS. Os espécimes foram processados para inclusão em glicol metacrilato (Leica Historesin, sendo aqueles com mais de 15 dias previamente descalcificados em EDTA. Para cada dia de desenvolvimento obteve-se cortes semi-seriados com 3 µm de espessura. As lâminas foram divididas em 2 grupos, sendo um grupo submetido à reação P. A. S. e o outro submetido à amilase salivar e em seguida à reação P. A. S. (grupo controle. Ao microscópio de luz foi observada, nas fases iniciais da odontogênese (12-15 dias, reação P. A. S. mais evidente nas células ectomesenquimais e aos 16-17 dias a reação predominou na região do folículo dental. Aos 18-19 dias a reação intensificou-se no retículo estrelado e aos 20 dias nas células do epitélio interno e na papila dental. De acordo com a metodologia empregada e com os resultados obtidos, concluímos que ocorre variação dos depósitos de glicogênio nas diferentes regiões do germe dental ao longo da odontogênese e que o Calomys callosus representa um modelo biológico para estudos da odontogênese

  8. Surveying a fossil oyster reef using terrestrial laser scanning

    Science.gov (United States)

    Haring, A.; Exner, U.; Harzhauser, M.

    2009-04-01

    The Korneuburg Basin, situated north-west of Vienna, is well known to contain a rich variety of fossils from the Early Miocene (16.5 ma) and therefore has been investigated extensively by scientists in the past decades. An exceptional discovery was made in 2005: a large fossil oyster reef has been excavated and documented carefully during the last years. Aside from the giant-sized oyster (Crassostrea gryphoides), the excavation site contains numerous species of molluscs along with teeth of sharks and rays and even isolated bones of sea cows. The oysters, having lengths of up to 80 cm, are protruding from the ground surface, which is more or less a tilted plane (25˚ ) with a size of about 300 m2. The entire site is crosscut by a network of geological faults, often also offsetting individual oyster shells. Displacements along the normal faults do not exceed ~ 15 cm. The faulted fossils offer a unique opportunity to measure displacement distribution along the faults in great detail and provide insight in deformation mechanisms in porous, barely lithified sediments. In order to get a precise 3D model of the oyster reef, the terrestrial laser scanner system Leica HDS 6000 is used. It is a phase-based laser scanner, i.e. the distance measurement is performed using the phase-shift principle. Compared to the time-of-flight principle, this method is generally more appropriate to projects like this one, where the distances to be measured are relatively small (aligned randomly or if they show a systematic alignment, in order to draw conclusions on the ocean currents at that time. For data processing, we use the 3D modeling software Geomagic Studio and the DTM (Digital Terrain Model) software SCOP++. The entire site has also been systematically documented by a large number of digital photographs. The obtained texture information allows a visual interpretation of the underlying surface. We discuss in how far such a 3D model derived from terrestrial laser scanner data may be

  9. Geodetic infrastructure at the Barcelona harbour for sea level monitoring

    Science.gov (United States)

    Martinez-Benjamin, Juan Jose; Gili, Josep; Lopez, Rogelio; Tapia, Ana; Pros, Francesc; Palau, Vicenc; Perez, Begona

    2015-04-01

    The presentation is directed to the description of the actual geodetic infrastructure of Barcelona harbour with three tide gauges of different technologies for sea level determination and contribution to regional sea level rise and understanding past and present sea level rise in the Barcelona harbour. It is intended that the overall system will constitute a CGPS Station of the ESEAS (European Sea Level) and TIGA (GPS Tide Gauge Benchmark Monitoring) networks. At Barcelona harbour there is a MIROS radar tide gauge belonging to Puertos del Estado (Spanish Harbours).The radar sensor is over the water surface, on a L-shaped structure which elevates it a few meters above the quay shelf. 1-min data are transmitted to the ENAGAS Control Center by cable and then sent each 1 min to Puertos del Estado by e-mail. The information includes wave forescast (mean period, significant wave height, sea level, etc.This sensor also measures agitation and sends wave parameters each 20 min. There is a GPS station Leica Geosystems GRX1200 GG Pro and antenna AX 1202 GG. The Control Tower of the Port of Barcelona is situated in the North dike of the so-called Energy Pier in the Barcelona harbor (Spain). This tower has different kind of antennas for navigation monitoring and a GNSS permanent station. As the tower is founded in reclaimed land, and because its metallic structure, the 50 m building is subjected to diverse movements, including periodic fluctuations due to temperature changes. In this contribution the 2009, 2011, 2012, 2013 and 2014 the necessary monitoring campaigns are described. In the framework of a Spanish Space Project, the instrumentation of sea level measurements has been improved by providing the Barcelona site with a radar tide gauge Datamar 2000C from Geonica S.L. in June 2014 near an acoustic tide gauge from the Barcelona Harbour installed in 2013. Precision levelling has been made several times in the last two years because the tower is founded in reclaimed land and

  10. The Correlation of Interphase Chromatin Structure with the Radiation-Induced Inter- and Intrachromosome Exchange Hotspots

    Science.gov (United States)

    Zhang, Ye; Mangala, Lingegowda S.; Purgason, Ashley M.; Hada, Megumi; Cucinotta, Francis A.; Wu, Honglu

    2011-01-01

    To investigate the relationship between chromosome aberrations induced by radiation and chromatin folding, we reconstructed three dimensional structure of chromosome 3 and measured the physical distances between different regions of the chromosome. Previously, we have investigated the location of breaks involved in inter- and intrachromosomal type exchange events in human chromosome 3, using the multicolor banding in situ hybridization (mBAND) technique. In human epithelial cells exposed to both low- and high-LET radiations in vitro, we reported that intra-chromosome exchanges occurred preferentially between a break in the 3p21 and one in the 3q11 regions, and the breaks involving in inter-chromosome exchanges occurred in two regions towards the telomeres of the chromosome. Exchanges were also observed between a break in 3p21 and one in 3q26, but few exchanges were observed between breaks in 3q11 and 3q26, even though the two regions are located on the same arm of the chromosome. In this study, human epithelial cells were fixed at G1 phase and the interphase cells were hybridized using the XCyte3 mBAND kit from MetaSystems. The z-section images of chromosome 3 were captured with a Leica and an LSM 510 Meta laser scanning confocal microscopes. A total of 100 chromosomes were analyzed. The reconstruction of three dimensional structure of interphase chromosome 3 with six different colored regions was achieved using the Imaris software. The relative distance between different regions was measured as well. We further analyzed fragile sites on the chromosome that have been identified in various types of cancers. The data showed that, in majority of the cells, the regions containing 3p21 and 3q11 are colocalized in the center of the chromosome, whereas, the regions towards the telomeres of the chromosome are either physically wrapping outside the chromosome center or with arms sticking out. Our results demonstrated that the distribution of breaks involved in radiation

  11. Multi-GNSS phase delay estimation and PPP ambiguity resolution: GPS, BDS, GLONASS, Galileo

    Science.gov (United States)

    Li, Xingxing; Li, Xin; Yuan, Yongqiang; Zhang, Keke; Zhang, Xiaohong; Wickert, Jens

    2017-10-01

    This paper focuses on the precise point positioning (PPP) ambiguity resolution (AR) using the observations acquired from four systems: GPS, BDS, GLONASS, and Galileo (GCRE). A GCRE four-system uncalibrated phase delay (UPD) estimation model and multi-GNSS undifferenced PPP AR method were developed in order to utilize the observations from all systems. For UPD estimation, the GCRE-combined PPP solutions of the globally distributed MGEX and IGS stations are performed to obtain four-system float ambiguities and then UPDs of GCRE satellites can be precisely estimated from these ambiguities. The quality of UPD products in terms of temporal stability and residual distributions is investigated for GPS, BDS, GLONASS, and Galileo satellites, respectively. The BDS satellite-induced code biases were corrected for GEO, IGSO, and MEO satellites before the UPD estimation. The UPD results of global and regional networks were also evaluated for Galileo and BDS, respectively. As a result of the frequency-division multiple-access strategy of GLONASS, the UPD estimation was performed using a network of homogeneous receivers including three commonly used GNSS receivers (TRIMBLE NETR9, JAVAD TRE_G3TH DELTA, and LEICA). Data recorded from 140 MGEX and IGS stations for a 30-day period in January in 2017 were used to validate the proposed GCRE UPD estimation and multi-GNSS dual-frequency PPP AR. Our results show that GCRE four-system PPP AR enables the fastest time to first fix (TTFF) solutions and the highest accuracy for all three coordinate components compared to the single and dual system. An average TTFF of 9.21 min with 7{°} cutoff elevation angle can be achieved for GCRE PPP AR, which is much shorter than that of GPS (18.07 min), GR (12.10 min), GE (15.36 min) and GC (13.21 min). With observations length of 10 min, the positioning accuracy of the GCRE fixed solution is 1.84, 1.11, and 1.53 cm, while the GPS-only result is 2.25, 1.29, and 9.73 cm for the east, north, and vertical

  12. Laser Scanning for the definition of high resolution topography in the Apuan Alps (IT) marble district

    Science.gov (United States)

    Riccucci, Silvia; Salvini, Riccardo; Francioni, Mirko

    2010-05-01

    The present paper describes the results of five laser scanning surveys performed by a Leica™ ScanStation2 with the aim of producing an high definition topography of a quarry, at the scale of 1:1,000. The project comes from a joint research carried out by the Authors in collaboration with the Local Sanitary Unit Agency (ASL n.1) of Massa and Carrara (IT). The main objective of this work has been the survey of both the exploited quarry walls and the upper residual and natural slopes. The area of interest is characterized by several quarry fronts which develop to variable and alternate directions to create very evident rock spurs, from NW-SE to NE-SW trending for a total wideness of about 2 hectares. Moreover, the quarry walls are sub-vertical and sometimes overhang by a total height of 100 m in respect to the quarry floor. Differential GPS and orthometric correction have been applied in order to co-register and to georeference the five point clouds; for these purposes a series of optical targets have been measured using a Laser Total Station. The subsequent phase has been the mesh construction and editing, from which a topographic map, 25 cm equidistance contours lines, has been created. The planimetric map shows the position and the geometry of crests, banks, escarpments, walls and all others exploitation features and the characteristics of the upper natural slopes. Morphological profiles along the maximum slope have been realized in order to better plan the future extractive activities according to the regional law. In order to make easier the prospective observation of detailed and overall areas, 3D views of multi-directional orientation have been realized. The produced data has been published by using the free LeicaTM TruView plug-in for Internet Explorer in a way to easily view the photographs and to measure the laser scan point clouds. Processing results have highlighted the higher spatial resolution of data coming from laser scanning in respect to the

  13. Hindlimb Suspension as a Model to Study Ophthalmic Complications in Microgravity Status Report: Optimization of Rat Retina Flat Mounts Staining to Study Vascular Remodeling

    Science.gov (United States)

    Theriot, Corey A.; Zanello, Susana B.

    2014-01-01

    Preliminary data from a prior tissue-sharing experiment has suggested that early growth response protein-1 (Egr1), a transcription factor involved in various stress responses in the vasculature, is induced in the rat retina after 14 days of hindlimb suspension (HS) and may be evidence that mechanical stress is occurring secondary to the cephalad fluid shift. This mechanical stress could cause changes in oxygenation of the retina, and the subsequent ischemia- or inflammation-driven hypoxia may lead to microvascular remodeling. This microvascular remodeling process can be studied using image analysis of retinal vessels and can be then be quantified by the VESsel GENeration Analysis (VESGEN) software, a computational tool that quantifies remodeling patterns of branching vascular trees and capillary or vasculogenic networks. Our project investigates whether rodent HS is a valid model to study the effects of simulated-weightlessness on ocular structures and their relationship with intracranial pressure (ICP). One of the hypotheses to be tested is that HS-induced cephalad fluid shift is accompanied by vascular engorgement that produces changes in retinal oxygenation, leading to oxidative stress, hypoxia, microvascular remodeling, and cellular degeneration. We have optimized the procedure to obtain flat mounts of rat retina, staining of the endothelial lining in vasculature and acquisition of high quality images suitable for VESGEN analysis. Briefly, eyes were fixed in 4% paraformaldehyde for 24 hours and retinas were detached and then mounted flat on microscope slides. The microvascular staining was done with endothelial cell-specific isolectin binding, coupled to Alexa-488 fluorophore. Image acquisition at low magnification and high resolution was performed using a new Leica SP8 confocal microscope in a tile pattern across the X,Y plane and multiple sections along the Z-axis. This new confocal microscope has the added capability of dye separation using the Linear

  14. RADIOMETRIC NORMALIZATION OF LARGE AIRBORNE IMAGE DATA SETS ACQUIRED BY DIFFERENT SENSOR TYPES

    Directory of Open Access Journals (Sweden)

    S. Gehrke

    2016-06-01

    Full Text Available Generating seamless mosaics of aerial images is a particularly challenging task when the mosaic comprises a large number of im-ages, collected over longer periods of time and with different sensors under varying imaging conditions. Such large mosaics typically consist of very heterogeneous image data, both spatially (different terrain types and atmosphere and temporally (unstable atmo-spheric properties and even changes in land coverage. We present a new radiometric normalization or, respectively, radiometric aerial triangulation approach that takes advantage of our knowledge about each sensor’s properties. The current implementation supports medium and large format airborne imaging sensors of the Leica Geosystems family, namely the ADS line-scanner as well as DMC and RCD frame sensors. A hierarchical modelling – with parameters for the overall mosaic, the sensor type, different flight sessions, strips and individual images – allows for adaptation to each sensor’s geometric and radiometric properties. Additional parameters at different hierarchy levels can compensate radiome-tric differences of various origins to compensate for shortcomings of the preceding radiometric sensor calibration as well as BRDF and atmospheric corrections. The final, relative normalization is based on radiometric tie points in overlapping images, absolute radiometric control points and image statistics. It is computed in a global least squares adjustment for the entire mosaic by altering each image’s histogram using a location-dependent mathematical model. This model involves contrast and brightness corrections at radiometric fix points with bilinear interpolation for corrections in-between. The distribution of the radiometry fixes is adaptive to each image and generally increases with image size, hence enabling optimal local adaptation even for very long image strips as typi-cally captured by a line-scanner sensor. The normalization approach is implemented in

  15. Evaluating the effectiveness of low cost UAV generated topography for geomorphic change detection

    Science.gov (United States)

    Cook, K. L.

    2014-12-01

    With the recent explosion in the use and availability of unmanned aerial vehicle platforms and development of easy to use structure from motion software, UAV based photogrammetry is increasingly being adopted to produce high resolution topography for the study of surface processes. UAV systems can vary substantially in price and complexity, but the tradeoffs between these and the quality of the resulting data are not well constrained. We look at one end of this spectrum and evaluate the effectiveness of a simple low cost UAV setup for obtaining high resolution topography in a challenging field setting. Our study site is the Daan River gorge in western Taiwan, a rapidly eroding bedrock gorge that we have monitored with terrestrial Lidar since 2009. The site presents challenges for the generation and analysis of high resolution topography, including vertical gorge walls, vegetation, wide variation in surface roughness, and a complicated 3D morphology. In order to evaluate the accuracy of the UAV-derived topography, we compare it with terrestrial Lidar data collected during the same survey period. Our UAV setup combines a DJI Phantom 2 quadcopter with a 16 megapixel Canon Powershot camera for a total platform cost of less than $850. The quadcopter is flown manually, and the camera is programmed to take a photograph every 5 seconds, yielding 200-250 pictures per flight. We measured ground control points and targets for both the Lidar scans and the aerial surveys using a Leica RTK GPS with 1-2 cm accuracy. UAV derived point clouds were obtained using Agisoft Photoscan software. We conducted both Lidar and UAV surveys before and after a summer typhoon season, allowing us to evaluate the reliability of the UAV survey to detect geomorphic changes in the range of one to several meters. We find that this simple UAV setup can yield point clouds with an average accuracy on the order of 10 cm compared to the Lidar point clouds. Well-distributed and accurately located ground

  16. Measuring Above Ground Biomass and Vegetation Structure in the South Florida Everglades Wetland Ecosystem with X-, C-, and L-band SAR data and Ground-based LiDAR

    Science.gov (United States)

    Feliciano, E. A.; Wdowinski, S.; Potts, M.; Chin, S.; Phillips, D. A.

    2010-12-01

    Worldwide, anthropogenic activities are disturbing and disrupting nutrient rich bio-diverse wetland ecosystems. Disturbance of the South Florida Everglades has been particularly acute, but difficult to quantify given its limited accessibility. Successful ecosystem monitoring requires the use of remote sensing. We used space-based Synthetic Aperture Radar (SAR) observations to estimate vegetation structure and above-ground biomass and track their changes over time. Our study leveraged three different SAR wavelengths that interact with different aspects of the vegetation. The short wavelength X-band (3.2 cm) signal interacts mainly with canopies; the intermediate wavelength C-band (5.6 cm) signal interacts with both canopies and branches; and the long wavelength L-band (24 cm) signal interacts with both the surface and lower portion of the vegetation. We used dual- and quadruple-polarization observations acquired from the TerraSAR-X, RadarSAT-2, and ALOS satellites. Different polarization data reflect radar signal interaction with different sections of the vegetation due to different scattering mechanisms. In order to calibrate the multi-wavelength and multi-polarization SAR observations, we conducted field measurement in three vegetation communities: hammock, pine and cypress. Our ground measurements included both traditional forestry surveys and state-of-the-art Terrestrial Laser Scanning (TLS), a.k.a. ground based LiDAR surveys. A week long TLS survey was conducted in the Everglades National Park in the three calibrations sites using a Leica ScanStation C10 TLS instrument which utilizes a narrow, green (532 nm) laser beam. During this week we collected a total of 29 scans (33 GB of data). The TLS surveys provided centimeter resolution 3-D point clouds of the ground surface and below-canopy vegetation. Initial analysis of the data has provided detailed 3-D estimates of the vegetation structure and above ground biomass. A comparative analysis of the ability of the

  17. High-rate (1 Hz to 20 Hz GPS coseismic dynamic displacements carried out during the Emilia 2012 seismic sequence

    Directory of Open Access Journals (Sweden)

    Antonio Avallone

    2012-10-01

    Full Text Available In May-July 2012, Emilia Romagna (northern Italy was struck by a significant seismic sequence, which was characterized by two moderate-magnitude earthquakes: a Ml 5.9 event on May 20, 2012, at 02:03:53 UTC, and a Ml 5.8 event on May 29, 2012, at 07:00:03 UTC, about 12 km to the west of the first mainshock. The earthquake sequence produced a total of 20 casualties and severe and widespread damage, mainly to historical and commercial buildings. A detailed description of the seismic sequence can be found in Sco-gnamiglio et al. [2012, this volume]. The largest of the earthquake static displacements were recorded by tens of continuous global positioning system (cGPS stations, as described in Serpelloni et al. [2012, this volume]. Most of these stations were operating with a sampling frequency of 1 Hz, and they belonged to scientific or commercial networks: RING (http://ring.gm.ingv.it; ITALPOS (http://smartnet.leica-geosystems.it; GeoTop (http://www.netgeo.it; Fondazione Geometri Emilia Romagna (http://www.gpsemiliaromagna.it; Lombardia [http://www.gpslombardia.it; and Veneto (http://147.162.229.63. Some hours after the first mainshock, the sampling frequency of the near-field RING stations (SBPO and MODE were switched to 20 Hz, thus recording the coseismic displacements produced by the May 29, 2012, earthquake at higher frequency. This sampling frequency was previously used for the detection of coseismic dynamic displacements only for the Mw 9 Tohoku-Oki 2011 event [Colosimo et al. 2011b]. Thus, the 20-Hz-sampling displacements for the Tohoku-Oki 2011 earthquake and the May 29, 2012, Emilia event might represent important recordings to investigate coseismic contributions at frequencies higher than 1 Hz with GPS. In the present study, after the description of the high-rate GPS (HRGPS data analysis, we will show and compare the preliminary results. Then, for the two mainshocks, we will compare the displacements recorded by the HRGPS (1 Hz up to 20 Hz

  18. Geologic and Geophysicsal Studies of Natural Hazards and Risks in the Gulf of Peter the Great, Japan Sea

    Science.gov (United States)

    Anokhin, Vladimir; Shcherbakov, Viktor; Motychko, Viktor; Slinchenkov, Vladimir; Sokolov, Georgy; Kotov, Sergey; Kartashov, Sergey

    2013-04-01

    The area of the Gulf of Peter the Great is socially, economically and culturally one of the most important regions for the Russian Far East. At the same time, there have been reported palpable natural hazards, which pose a real threat to local infrastructure. Complex field team of the Gramaberg VNIIOkeangeologia institute carried out geological and geophysical studies of natural hazards in the water area and coastal zone of the gulf in the summer and autumn of 2012. The research program included - geodetic deformation monitoring of the coastal zone by the HDS 3000 Leica tachometer; - echo sounding of the underwater part of the coastal slope by the LCX-37C depth sounder equipped with active external 12-channel GPS Lowrance antenna LGC-3000; - high-frequency acoustic profiling by GeoPulse Subbotom Profilier with oscillator frequency of 12.2 kHz for the study of bottom sediments to a depth of 40 m; - hydromagnetic measurements by SeaSPY Marine Magnetics magnetometer for investigation of deep geological structure; - sonar measurements by GEO SM C-MAX, 325 kHz frequency emitters for studying seafloor features; - studies of the water column (sensing and sampling); - bottom sediment sampling. Analytic work was performed by mass spectrometry, atomic absorption spectrophotometry, chromatography, gas chromatography-mass spectrometry, gamma spectrometry and included the following. For water - the content of Fe, Mn, Cd, As, Pb, Cu, Co, Ni, Cr, Zn, Hg in solution and in suspension, polycyclic aromatic compounds, organochlorine pesticides, oil, methane. For sediments - grade analysis, mineralogical analysis of sand, determination of Fe, Mn, Cd, As, Pb, Cu, Co, Ni, Cr, Zn, Hg content; identification of petroleum products, polychlorinated biphenyls, organochlorine pesticides, the specific activity of Cs-137. As a result, a set of geological maps was composed: maps of pre-Quaternary and Quaternary rocks and deposits, lithological map, geomorphological map, map of engineering

  19. Calcareous nannofossils of the Toarcian-Aalenian transition in the São Gião section (Lusitanian Basin, Portugal): preliminary results

    Science.gov (United States)

    Cortesão, André; Henriques, Maria Helena

    2016-04-01

    This work presents preliminary results regarding the composition of the calcareous nannofossils assemblages' recorded in the Lower-Middle Jurassic transition of the São Gião section, located in the northern Lusitanian Basin (Central Portugal). The section is a 45 m-thick monotonous alternation of marl and marly limestone (the Póvoa da Lomba Formation) ranging from the Upper Toarcian to the Lower Aalenian, and it corresponds to an expanded section showing exceptional exposure conditions. The continuous record of ammonites has enabled the recognition of the Aalensis Zone (Mactra and Aalensis subzones) and the Opalinum Zone (Opalinum and Comptum subzones). The abundant and very diverse benthic foraminiferal record accurately calibrated with the ammonite record has allowed the recognition of the Astacolus dorbignyi Zone. For the study of the calcareous nannofossil record, four samples were collected (one for each ammonite subzone) and processed; the smear slides were analyzed in a Leica DM750P polarizing microscope, using a 1000 X magnification. The nannfossil assemblages of the São Gião section are dominated by representatives of the genera Lotharingius and Discorhabdus, whereas Carinolithus and Schizosphaerella are subordinated. Other genera also represented in the analysed assemblages include Crepidolithus and Thoracosphaera. As noticed for the ammonite and for the benthic foraminiferal record, throughout the Upper Toarcian - Lower Aalenian record for the São Gião section no drastic changes in the number of originations and extinctions of nannofossil genera between ammonite biozones was detected. The main faunal change is the increase in the relative abundance of the genera Carinolithus in the Comptum Subzone, and the concomitant reduction of the relative abundance of Discorhabdus, but Lotharingius representatives remain dominant during the whole Upper Toarcian - Lower Aalenian transition. Further developments on this study will contribute to elaborate an

  20. Scanning Apollo Flight Films and Reconstructing CSM Trajectories

    Science.gov (United States)

    Speyerer, E.; Robinson, M. S.; Grunsfeld, J. M.; Locke, S. D.; White, M.

    2006-12-01

    Over thirty years ago, the astronauts of the Apollo program made the journey from the Earth to the Moon and back. To record their historic voyages and collect scientific observations many thousands of photographs were acquired with handheld and automated cameras. After returning to Earth, these films were developed and stored at the film archive at Johnson Space Center (JSC), where they still reside. Due to the historical significance of the original flight films typically only duplicate (2nd or 3rd generation) film products are studied and used to make prints. To allow full access to the original flight films for both researchers and the general public, JSC and Arizona State University are scanning and creating an online digital archive. A Leica photogrammetric scanner is being used to insure geometric and radiometric fidelity. Scanning resolution will preserve the grain of the film. Color frames are being scanned and archived as 48 bit pixels to insure capture of the full dynamic range of the film (16 bit for BW). The raw scans will consist of 70 Terabytes of data (10,000 BW Hasselblad, 10,000 color Hasselblad, 10,000 Metric frames, 4500 Pan frames, 620 35mm frames counts; are estimates). All the scanned films will be made available for download through a searchable database. Special tools are being developed to locate images based on various search parameters. To geolocate metric and panoramic frames acquired during Apollos 15\\-17, prototype SPICE kernels are being generated from existing photographic support data by entering state vectors and timestamps from multiple points throughout each orbit into the NAIF toolkit to create a type 9 Spacecraft and Planet Ephemeris Kernel (SPK), a nadir pointing C\\- matrix Kernel (CK), and a Spacecraft Clock Kernel (SCLK). These SPICE kernels, in addition to the Instrument Kernel (IK) and Frames Kernel (FK) that also under development, will be archived along with the scanned images. From the generated kernels, several IDL

  1. DIRECT GEOREFERENCING OF UAV DATA BASED ON SIMPLE BUILDING STRUCTURES

    Directory of Open Access Journals (Sweden)

    W. Tampubolon

    2016-06-01

    effective tool for the LSTM application at low cost. Our results show that image orientation calculations from building structure essentially improve the accuracy of direct georeferencing procedure adjusted also by the GCPs. To gain three dimensional (3D point clouds in local coordinate system, an extraction procedure has been performed by using Agisoft Photo Scan. Subsequently, a Digital Surface Model (DSM generated from the acquired data is the main output for LSTM that has to be assessed using standard field and conventional mapping workflows. For an appraisal, our DSM is compared directly with a similar DSM obtained by conventional airborne data acquisition using Leica RCD-30 metric camera as well as Trimble Phase One (P65+ camera. The comparison reveals that our approach can achieve meter level accuracy both in planimetric and vertical dimensions.

  2. Virtual slides in peer reviewed, open access medical publication

    Directory of Open Access Journals (Sweden)

    Kayser Klaus

    2011-12-01

    Full Text Available Abstract Background Application of virtual slides (VS, the digitalization of complete glass slides, is in its infancy to be implemented in routine diagnostic surgical pathology and to issues that are related to tissue-based diagnosis, such as education and scientific publication. Approach Electronic publication in Pathology offers new features of scientific communication in pathology that cannot be obtained by conventional paper based journals. Most of these features are based upon completely open or partly directed interaction between the reader and the system that distributes the article. One of these interactions can be applied to microscopic images allowing the reader to navigate and magnify the presented images. VS and interactive Virtual Microscopy (VM are a tool to increase the scientific value of microscopic images. Technology and Performance The open access journal Diagnostic Pathology http://www.diagnosticpathology.org has existed for about five years. It is a peer reviewed journal that publishes all types of scientific contributions, including original scientific work, case reports and review articles. In addition to digitized still images the authors of appropriate articles are requested to submit the underlying glass slides to an institution (DiagnomX.eu, and Leica.com for digitalization and documentation. The images are stored in a separate image data bank which is adequately linked to the article. The normal review process is not involved. Both processes (peer review and VS acquisition are performed contemporaneously in order to minimize a potential publication delay. VS are not provided with a DOI index (digital object identifier. The first articles that include VS were published in March 2011. Results and Perspectives Several logistic constraints had to be overcome until the first articles including VS could be published. Step by step an automated acquisition and distribution system had to be implemented to the corresponding

  3. Role of Ki-67 proliferation index in the assessment of patients with neuroendocrine neoplasias regarding the stage of disease.

    Science.gov (United States)

    Miller, H C; Drymousis, P; Flora, R; Goldin, R; Spalding, D; Frilling, A

    2014-06-01

    Neuroendocrine neoplasias (NEN) of the gastroenteropancreatic (GEP) system frequently present with metastatic deposits. The proliferation marker Ki-67 is used for diagnosis and to assess the prognosis of disease. The aim of our study was to evaluate the usefulness of Ki-67 % in the assessment of NEN patients with regard to their disease stage in clinical practice. Additionally, a comparative analysis of Ki-67 levels among different sites of disease was performed. This retrospective study included patients with GEP NEN referred to our center from 2010 to 2012. The NEN diagnosis was confirmed by standard histopathology. Ki-67 immunohistochemistry was done on paraffin-embedded sections using an automated Leica immunohistochemistry machine. NEN grading was carried out according to European Neuroendocrine Tumor Society recommendations (low grade [G1] to intermediate grade [G2], well to moderately differentiated neuroendocrine neoplasms; high-grade [G3], moderately to poorly differentiated neuroendocrine neoplasms). Results of tumor staging and grading were correlated. In a subgroup of cases, comparative analysis of Ki-67 levels in different sites of disease was carried out. One hundred sixty-one GEP NEN patients were included in the study. Metastatic disease was seen in 46.1 % (53/115) of G1 tumors, 77.8 % (28/36) of G2 tumors, and 100 % of (10/10) G3 tumors (p = 0.0002). When stratified according to primary tumor site, metastatic disease was documented in 42.9 % (36/84) of patients with pancreatic NEN and in 91.9 % (34/37) of those with small intestinal primary. Stage IV metastatic disease was present in 27.8 % (32/115) and 72.2 % (26/36) of the G1 and G2 tumors, respectively, and in 90 % (9/10) of the G3 tumors. Assessment of the Ki-67 index for a subset of cases at metastatic sites as well as the primary tumor site showed discrepancies in 35.3 % cases. In 7/9 (77.8 %) patients with liver metastases, Ki-67 % was higher in the liver lesions than in the primary tumor

  4. The effect of iodine and strumigens long-term foodborne intake on histometrical parameters of thyroid gland in gimmers

    Directory of Open Access Journals (Sweden)

    Zdeněk Peksa

    2013-01-01

    Full Text Available In 2010 and 2011 two experiments on gimmers of Šumava mountain sheep were performed. The first experiment was carried out on 12 animals (experimental period was 11 months; control group was fed with 3 mg I*kg−1 in dry matter per day and experimental group was fed with 5 mg I*kg−1 in dry matter per day. The second experiment lasted 10 months and it was carried out on 12 animals. Feed ration for control group contained 10 mg I*kg DM−1. Feed ration for experimental group contained 10 mg I*kg DM−1 too; moreover it included rapeseed meal and 1 g of sodium nitrate. The aim of the first experiment was focused on impact of high iodine intake on structure of thyroid gland. The aim of the second experiment was to discover effect of strumigens during high iodine intake on structure of thyroid gland. The animals were slaughtered after the experiment and there was executed the dissection of thyroid gland. The samples of thyroid gland were processed during classic paraffin method and dyed with haematoxylin and eosin. For finding of histometrical parameters was used program Leica IM 500 Version 4.0. The length, the width and the area of follicles were measured. The follicles were divided into three groups after this procedure (by the length; in each group were measured 20 thyreocytes. In group with intake 5 mg I*kg DM−1 (experiment from year 2010: higher weight of thyroid (p < 0.01, significant higher (p < 0.05 proportional representation of large and medium follicles, significantly (p < 0.05 higher average size of follicles, demonstrably lower (p < 0.05 height of epithelium were found. There were not found any differences in monitored parameters between the groups from experiment which was carried out during the year 2011. After comparing results from the both experiments significantly higher percentage representation of large follicles a significantly lower representation of small follicles, distinctly higher average size and higher

  5. GATA3: a multispecific but potentially useful marker in surgical pathology: a systematic analysis of 2500 epithelial and nonepithelial tumors.

    Science.gov (United States)

    Miettinen, Markku; McCue, Peter A; Sarlomo-Rikala, Maarit; Rys, Janusz; Czapiewski, Piotr; Wazny, Krzysztof; Langfort, Renata; Waloszczyk, Piotr; Biernat, Wojciech; Lasota, Jerzy; Wang, Zengfeng

    2014-01-01

    GATA3 is a transcription factor important in the differentiation of breast epithelia, urothelia, and subsets of T lymphocytes. It has been suggested to be useful in the evaluation of carcinomas of mammary or urothelial origin or metastatic carcinomas, but its distribution in normal and neoplastic tissues is incompletely mapped. In this study, we examined normal developing and adult tissues and 2040 epithelial and 460 mesenchymal or neuroectodermal neoplasms for GATA3 expression to explore its diagnostic value in surgical pathology, using monoclonal antibody (clone L50-823) and Leica Bond automated immunohistochemistry. GATA3 was expressed in trophoblast, fetal and adult epidermis, adult mammary and some salivary gland and sweat gland ductal epithelia, urothelia, distal nephron in developing and adult tissues, some prostatic basal cells, and subsets of T lymphocytes. It was expressed stronger in fetal than in adult mesothelia and was absent in respiratory and gastrointestinal epithelia. In epithelial neoplasms, GATA3 was expressed in >90% of primary and metastatic ductal and lobular carcinomas of the breast, urothelial, and cutaneous basal cell carcinomas and trophoblastic and endodermal sinus tumors. In metastatic breast carcinomas, it was more sensitive than GCDFP. Among squamous cell carcinomas, the expression was highest in the skin (81%) and lower in cervical (33%), laryngeal (16%), and pulmonary tumors (12%). Common positivity was found in skin adnexal tumors (100%), mesothelioma (58%), salivary gland (43%), and pancreatic (37%) ductal carcinomas, whereas frequency of expression in adenocarcinomas of lung, stomach, colon, endometrium, ovary, and prostate was <10%. Chromophobe renal cell carcinoma was a unique renal tumor with frequent positivity (51%), whereas oncocytomas were positive in 17% of cases but other types only rarely. Among mesenchymal and neuroectodermal tumors, paragangliomas were usually positive, which sets these tumors apart from epithelial

  6. Study into Point Cloud Geometric Rigidity and Accuracy of TLS-Based Identification of Geometric Bodies

    Science.gov (United States)

    Klapa, Przemyslaw; Mitka, Bartosz; Zygmunt, Mariusz

    2017-12-01

    Capability of obtaining a multimillion point cloud in a very short time has made the Terrestrial Laser Scanning (TLS) a widely used tool in many fields of science and technology. The TLS accuracy matches traditional devices used in land surveying (tacheometry, GNSS – RTK), but like any measurement it is burdened with error which affects the precise identification of objects based on their image in the form of a point cloud. The point’s coordinates are determined indirectly by means of measuring the angles and calculating the time of travel of the electromagnetic wave. Each such component has a measurement error which is translated into the final result. The XYZ coordinates of a measuring point are determined with some uncertainty and the very accuracy of determining these coordinates is reduced as the distance to the instrument increases. The paper presents the results of examination of geometrical stability of a point cloud obtained by means terrestrial laser scanner and accuracy evaluation of solids determined using the cloud. Leica P40 scanner and two different settings of measuring points were used in the tests. The first concept involved placing a few balls in the field and then scanning them from various sides at similar distances. The second part of measurement involved placing balls and scanning them a few times from one side but at varying distances from the instrument to the object. Each measurement encompassed a scan of the object with automatic determination of its position and geometry. The desk studies involved a semiautomatic fitting of solids and measurement of their geometrical elements, and comparison of parameters that determine their geometry and location in space. The differences of measures of geometrical elements of balls and translations vectors of the solids centres indicate the geometrical changes of the point cloud depending on the scanning distance and parameters. The results indicate the changes in the geometry of scanned objects

  7. Integrating wireless sensor network for monitoring subsidence phenomena

    Science.gov (United States)

    Marturià, Jordi; Lopez, Ferran; Gigli, Giovanni; Intrieri, Emanuele; Mucchi, Lorenzo; Fornaciai, Alessandro

    2016-04-01

    An innovative wireless sensor network (WSN) for the 3D superficial monitoring of deformations (such as landslides and subsidence) is being developed in the frame of the Wi-GIM project (Wireless sensor network for Ground Instability Monitoring - LIFE12 ENV/IT/001033). The surface movement is detected acquiring the position (x, y and z) by integrating large bandwidth technology able to detect the 3D coordinates of the sensor with a sub-meter error, with continuous wave radar, which allows decreasing the error down to sub-cm. The Estació neighborhood in Sallent is located over the old potassium mine Enrique. This zone has been affected by a subsidence process over more than twenty years. The implementation of a wide network for ground auscultation has allowed monitoring the process of subsidence since 1997. This network consists of: i) a high-precision topographic leveling network to control the subsidence in surface; ii) a rod extensometers network to monitor subsurface deformation; iii) an automatic Leica TCA Total Station to monitor building movements; iv) an inclinometers network to measure the horizontal displacements on subsurface and v) a piezometer to measure the water level. Those networks were implemented within an alert system for an organized an efficient response of the civil protection authorities in case of an emergency. On 23rd December 2008, an acceleration of subsoil movements (of approx. 12-18 cm/year) provoked the activation of the emergency plan by the Catalan Civil Protection. This implied the preventive and scheduled evacuation of the neighbours (January 2009) located in the area with a higher risk of collapse: around 120 residents of 43 homes. As a consequence, the administration implemented a compensation plan for the evacuation of the whole neighbourhood residents and the demolition of 405 properties. In this work, the adaptation and integration process of Wi-GIM system with those conventional monitoring network are presented for its testing

  8. Forest structure analysis combining laser scanning with digital airborne photogrammetry

    Science.gov (United States)

    Lissak, Candide; Onda, Yuichi; Kato, Hiroaki

    2017-04-01

    The interest of Light Detection and Ranging (LiDAR) for vegetation structure analysis has been demonstrated in several research context. Indeed, airborne or ground Lidar surveys can provide detailed three-dimensional data of the forest structure from understorey forest to the canopy. To characterize at different timescale the vegetation components in dense cedar forests we can combine several sources point clouds from Lidar survey and photogrammetry data. For our study, Terrestrial Laser Scanning (TLS-Leica ScanStation C10 processed with Cyclone software) have been lead in three forest areas (≈ 200m2 each zone) mainly composed of japanese cedar (Japonica cryptomeria), in the region of Fukushima (Japan). The study areas are characterized by various vegetation densities. For the 3 areas, Terrestrial laser scanning has been performed from several location points and several heights. Various floors shootings (ground, 4m, 6m and 18m high) were able with the use of a several meters high tower implanted to study the canopy evolution following the Fukushima Daiishi nuclear power plant accident. The combination of all scanners provides a very dense 3D point cloud of ground and canopy structure (average 300 000 000 points). For the Tochigi forest area, a first test of a low-cost Unmanned Aerial Vehicle (UAV) photogrammetry has been lead and calibrated by ground GPS measurements to determine the coordinates of points. TLS combined to UAV photogrammetry make it possible to obtain information on vertical and horizontal structure of the Tochigi forest. This combination of technologies will allow the forest structure mapping, morphometry analysis and the assessment of biomass volume evolution from multi-temporal point clouds. In our research, we used a low-cost UAV 3 Advanced (200 m2 cover, 1300 pictures...). Data processing were performed using PotoScan Pro software to obtain a very dense point clouds to combine to TLS data set. This low-cost UAV photogrammetry data has been

  9. Innovazione tecnologica e sinergie tra soluzioni geospaziali Lo "scenario Intergraph"

    Directory of Open Access Journals (Sweden)

    Andrea Fiduccia

    2012-04-01

    Full Text Available Negli ultimi anni abbiamo potuto assistere all’espansione delle tecnologie per l’Informazione Geografica dall’originale ambito della cartografia numerica per la conoscenza del territorio (automated mapping o per la gestione delle infrastrutture e delle reti tecnologiche (facility management verso un dominio più ampio denominato "geospatial".Technological innovation and synergies between geospatial solutions. The "Intergraph's scenario".Geospatial  is  much  more  than  GIS.  We  can  understand  the evolution  of  Geographic  Information  considering  some  new working groups and OWS demonstrations of Open Geospa-tial Consortium: Sensor Web Enablement, Sensor Fusion Ena-blement, Feature & Decision Fusion, Aviation, Emergency and Disaster Management, etc. Another point of observation is to analyze some brand new products of a multinational enterprise committed to the innovation like Intergraph Corporation. Part of Hexagon AB, Intergraph, Leica Geosystems and ERDAS are working together to leverage joint strengths in geospatial in-novation.Intergraph’s Motion Video Exploitation solution leverages full motion video, giving analysts the ability to collect, analyze, and maximize the value of video assets.GeoMedia  3D  is  a  GeoMedia  add-on  product  that  extends the functionality of Intergraph’s geospatial solutions through an integrated 3D visualization and analysis environment. You can visualize, navigate, analyze, and interact with 3D data na-tively in GeoMedia.GeoMedia Smart Client delivers an enterprise geospatial plat-form engineered to support large numbers of users who are unable to operate full desktop products, but whose workflows need  advanced  geospatial  functionality  that  cannot  be  sup-ported by Web mapping tools.G/Technology Fiber Optic Works 1.0 streamlines the management  of  fiber  optic  infrastructure  for  utilities,  municipalities, agencies and

  10. Innovazione tecnologica e sinergie tra soluzioni geospaziali Lo "scenario Intergraph"

    Directory of Open Access Journals (Sweden)

    Andrea Fiduccia

    2012-04-01

    Full Text Available Negli ultimi anni abbiamo potuto assistere all’espansione delle tecnologie per l’Informazione Geografica dall’originale ambito della cartografia numerica per la conoscenza del territorio (automated mapping o per la gestione delle infrastrutture e delle reti tecnologiche (facility management verso un dominio più ampio denominato "geospatial". Technological innovation and synergies between geospatial solutions. The "Intergraph's scenario".Geospatial  is  much  more  than  GIS.  We  can  understand  the evolution  of  Geographic  Information  considering  some  new working groups and OWS demonstrations of Open Geospa-tial Consortium: Sensor Web Enablement, Sensor Fusion Ena-blement, Feature & Decision Fusion, Aviation, Emergency and Disaster Management, etc. Another point of observation is to analyze some brand new products of a multinational enterprise committed to the innovation like Intergraph Corporation. Part of Hexagon AB, Intergraph, Leica Geosystems and ERDAS are working together to leverage joint strengths in geospatial in-novation.Intergraph’s Motion Video Exploitation solution leverages full motion video, giving analysts the ability to collect, analyze, and maximize the value of video assets.GeoMedia  3D  is  a  GeoMedia  add-on  product  that  extends the functionality of Intergraph’s geospatial solutions through an integrated 3D visualization and analysis environment. You can visualize, navigate, analyze, and interact with 3D data na-tively in GeoMedia.GeoMedia Smart Client delivers an enterprise geospatial plat-form engineered to support large numbers of users who are unable to operate full desktop products, but whose workflows need  advanced  geospatial  functionality  that  cannot  be  sup-ported by Web mapping tools.G/Technology Fiber Optic Works 1.0 streamlines the management  of  fiber  optic  infrastructure  for  utilities,  municipalities, agencies and

  11. BUILDING EXTRACTION USING MULTI SENSOR SYSTEMS

    Directory of Open Access Journals (Sweden)

    N. Yastikli

    2013-05-01

    Full Text Available In this study, the automatic building extraction is aimed using object-based image analysis method with multi sensor system includes LiDAR, digital camera and GPS/IMU. The image processing techniques, segmentation and classification methods were used for automatic object extraction with defined rule set. The proposed method based on object based classification to overcome the limitation of traditional pixel based classification such as confusion of classes. The generated Digital Surface Model (DSM from LiDAR point cloud was used to separate building and vegetation classes. The morphologic filters were utilized also optimization of mixed classes. In our proposed approach for building extraction, multi-resolution, contrast-difference and chessboard segmentations were applied. The object-based classification method was preferred in classification process with defined fuzzy rules. First, vegetation and ground classes were generated than building regions were derived with using the results of the classification and segmentation. The data set was obtained from the project of "NABUCCO Gas Pipeline Project". The data set actually was collected for corridor mapping of pipeline which will link the Eastern border of Turkey, to Baumgarten in Austria via Bulgaria, Romania and Hungary. The study area is a suburban neighborhood located in the city of Sivas, Turkey. The Leica ALS60 LiDAR system, DiMAC, Dalsa Area Bayer RGB Charge Coupled (CCD Camera and GPS and CUS6 IMU system were used for data collection. The additional data sets were generated with point cloud collected by LiDAR and RGB images from digital camera. The rule sets for automatic building extraction were developed in Definiens e-Cognition Developer 8.64 program system. To evaluate the performance of proposed automatic building extraction approach, reference data set was generated with digitizing of extracted building over the orthoimage. The accuracy assessment was performed with completeness and

  12. Terrestrial photography as method to identify sediment sources in eroding rills

    Science.gov (United States)

    Wirtz, Stefan; Remke, Alexander; Wengel, René; Seeger, Manuel; Ries, Johannes B.

    2013-04-01

    One weak point in available studies on erosion processes in eroding rills is the lack of information about the real source of transported sediment. So far, such sources can only be identified by observation during the event or the experiment. Quantification is not possible by this way, additionally only large and clear visible changes are considered. In this study we present a method to quantify even small topographical changes in eroding rills during experiments or real rainfall events. We tested the suitability of DEMs created from terrestrial stereo photographies before and after application of artificial runoff to quantify eroded, transported and sedimented material in the rill. In 1 m intervals, pairs of photos are taken along the tested rills. The setup ensures that both cameras are moved with a constant parallax. The height of only about 1 m above ground level ensures that even low quantities of moved material can be identified. Based on technical data of the camera and the shot level, the theoretical accuracy of the images is below 1 mm horizontal ground resolution. The camera carrier bases on a rotating tower crane which has been developed from a customary survey tripod. The stereo images are combined with free-hand photographies from different angles of view to reach overhangs in rill sidewalls and ensure a fully 3D representation of the rill. In the analyses of the photographies 2 different kinds of software are used: The standard software Leica Photogrammetry Suite requires stereo images and ground control points whereas the open source software package is able to handle either stereo images as well as free-hand photographies. Ground control points are not needed, the software identifies point clusters which are to find in overlapping images. Using these feature-points, spatial marks are positioned which are used to match and rectify the single images to get an apposite panorama. By this way images from very different angles of view can be combined. The

  13. Performance evaluation of laser guided leveler

    Directory of Open Access Journals (Sweden)

    M.A. Hoque

    2014-12-01

    Full Text Available The study was conducted at Bangladesh Agricultural Research Institute (BARI farm on clay loam soil during Rabi season of 2010-2011. The treatments consisted of laser land leveling (T1 and control (non-leveled (T2. A preliminary field survey was done using staff gage. Initially a base station was established to dispense laser ray uniformly. The laser ray erected from base station guided the sensor of the stuff gage and the leveler. Elevation data was collected from the different points of the field and made an average. The maximum gage reading were 247.0 cm and the minimum gage reading was 219.2 cm. Average gage readings of the laser leveled plot was 235.66 cm that was settled for auto adjustment. Therefore, huge amount of soils (16.46 cm high was cut from the highest point and subsequently had to fill to the low points. Finally, an equal gage reading of 235.66 cm was observed after leveling the plot. The laser leveler (Leica MLS700 was used hitching with a TAFE tractor. The field was leveled with manual control initially and finally it was operated with auto adjustment. Two operators, 25 litter diesels and total 6 hours time were required during this leveling. Wheat was cultivated in leveled land (T1 and non-leveled land (T2. Laser leveling was insured for improvement in nutrient use efficiencies, option for precision farming, reduces weed problems, and improves uniformity of crop maturity. There was better distribution of water in leveled plot, which helped to reduce irrigation application time 1 hour. Due to uniformity of moisture content improved germination and crop establishment was found which reflected in higher plant population (239 m-2. Maximum yield (3.41 t ha-1 was obtained in T1 due to longer panicle (10.89 cm, more grain per plant (27.47 and 1000 grain weight (47.38 g compared to yield of T2 (2.62 t ha-1.

  14. Towards effective and stable probiotics.

    Science.gov (United States)

    Yarullina, D R; Damshkaln, L G; Bruslik, N L; Konovalova, O A; Ilinskaya, O N; Lozinsky, V I

    2015-01-01

    -MDT, Moscow, Russia).Starch swelling and dissolution was studied in simulated colonic fluid (SCF), prepared according to [3] and in distilled water (pH = 6.0) as control. Amylase from Aspergillus oryzae (A8220, Sigma) was added to the solutions to study the influence of amylase. The formulation form was examined visually during 14 h incubation time.Fluorescence microscopy images were obtained with a Leica DM6000B (Germany) fluorescent microscope using Leica FW4000 software.L. plantarum 8PA3 loaded in SPS were placed either in HCl solution (pH 2), or in 2% oxgall bile solution, or in 0.85% NaCl solution. Viability was tested after 2, 4 and 6 h incubation at 37°C by plating diluted aliquots onto MRS agar with subsequent counting of bacterial colony forming units (CFU). In addition, viability was determined using LIVE/DEAD BacLight bacterial viability kit L-7012 (Molecular Probes, Invitrogen) as described elsewhere [4]. Fluorescence in the stained samples was estimated with BD FACS Canto II (USA) flow cytometer or fluorescent microscope.Nitric oxide (NO) production was assessed with DAF-FM DA and DAA fluorescent dyes as described earlier [4]. Each experiment was performed in triplicate. In the present study we studied the probiotic composition comprising of SPS and bacteria L. plantarum 8PA3. We used AFM to confirm effective fixation of the cells to carbohydrate. The compositions were found to swell quickly (~5 min) in aqueous solutions either containing amylase, or not. Tested starch formulations disintegrated during the first 5-10 min of incubation in amylase solutions whereas in amylase-free probes dissolution was less intensive (after ~30 min). Amylolysis of starch excipients was less pronounced in aqueous amylase solution than in SCF, supplemented with amylase. None of 19 studied Lactobacillus strains hydrolyzed SPS when growing on MRS agar supplemented with it. The amount of viable L. plantarum 8PA3 cells formulated with SPS was high and did not change when stored for 6

  15. Physical incorporation of particles in a porous media: a path to a smart wood

    Science.gov (United States)

    Zerriaa, Azza; El Ganaoui, Mohammed; Gerardin, Christine; Tazibt, Abdel; Gabsi, Slimane

    2016-05-01

    The aim of this work is to develop a functional wood incorporating, in its surface, physical and chemical properties that meet society demand. For instance: fire resistance, magnetic electrical conduction (metal-wood particles), antibacterial reaction (copper-wood), anti-pollution (zeolite-wood), dry coloring, reflective effects (minerals-wood). As part of the research on wooden materials, the technique of "JAZOLTHOP1" driving micrometric particles before combining them and moving in supersonic speeds was used in the framework of enriching a wooden substrate. Various tests were conducted on two wooden materials (fir and ash tree) submitted to four typologies of particles (steel shot, garnet, corundum and glass beads). The surfaces of the test samples were machined beforehand for a use of conventional smooth quality, thus defining a reference surface before incorporation. The enriched samples were characterized by using two optical techniques; firstly a surface technique through macroscopy Leica 110X ZP, then a volume technique through tomography2. Subsequently, volume simulations (wood-inclusions) were implemented to study the thermal transfer. The obtained results showcase the existence of certain set conditions to reach the critical fluency of incorporation and to localize the enrichment on a parallel plan to the sample surface. The results show also the influence of particles concentration and the kind of the chosen wood on the final composite matrix/particle media. Contribution to the topical issue "Materials for Energy Harvesting, Conversion and Storage (ICOME 2015) - Elected submissions", edited by Jean-Michel Nunzi, Rachid Bennacer and Mohammed El Ganaoui http:// http:// http:// http:// http:// http://Nunzihttp:// http://Jean-Michelhttp:// http:// http:// http:// http:// http://Bennacerhttp:// http://Rachidhttp:// http:// http:// http:// http:// http://El Ganaouihttp:// http://Mohammedhttp:// http:// http:// http://Guest editorshttp:// http:// http:// http

  16. Bandagem reversível do tronco pulmonar: modelo experimental para preparo rápido do ventrículo pulmonar

    Directory of Open Access Journals (Sweden)

    ASSAD Renato S.

    1998-01-01

    Full Text Available Um novo cateter-balão foi desenvolvido com o objetivo de induzir o preparo rápido do ventrículo pulmonar. O cateter apresenta três vias, uma para o balão e duas para medir pressões, proximal e distal ao balão [ventrículo direito (VD e tronco pulmonar (TP]. Os corações de 6 cabritos jovens (peso médio: 5,3 kg foram submetidos à sobrecarga sistólica imposta pelo cateter e avaliados morfologicamente e pelo ecocardiograma. A via de saída do VD (VSVD foi exposta através de toracotomia esquerda. Foram realizadas biópsias do miocárdio para estudos de microscopia óptica e eletrônica. O cateter-balão foi introduzido pela VSVD e posicionado no TP. Após a convalecença pós-operatória, foi iniciado o treinamento do VD através da injeção de 0,5 ml de água no balão. Posteriormente, volumes adicionais (0,5 ml eram injetados no balão a cada 2 dias, causando sobrecarga sistólica progressiva. A avaliação ecocardiográfica foi realizada com intervalos de 1 a 2 dias. Os animais foram sacrificados após 2 a 3 semanas de treinamento do VD, para avaliação morfológica do coração. O diâmetro externo dos miócitos cardíacos, seccionados longitudinalmente, foi medido ao nível do núcleo, utilizando-se o sistema de análise de imagem (Quantimet-Leica. Secções de 1µ de espessura do VD foram examinados sob microscopia eletrônica para determinar a densidade de volume das mitocôndrias. O ecocardiograma revelou equalização das massas musculares dos ventrículos com intervalo de 6 a 10 dias de treinamento do VD. À microscopia óptica, foi observado aumento significativo do diâmetro dos miócitos (p<0,001 e dos núcleos (p<0,003, embora sob a microscopia eletrônica não tenha havido variação significativa da densidade média do volume de mitocôndrias do VD (p=0,385. O cateter-balão proposto neste trabalho permite a manipulação da luz do TP, possibilitando o preparo rápido e não cirúrgico do ventrículo pulmonar e

  17. PREFACE: 17th International Conference on Microscopy of Semiconducting Materials 2011

    Science.gov (United States)

    Walther, T.; Midgley, P. A.

    2011-11-01

    where necessary. In the end, 61 manuscripts were accepted for publication. The Editors are very grateful to the following colleagues for their rapid and careful reviewing of manuscripts: M Albrecht, J S Barnard, R Beanland, G A Botton, D Cooper, L Clement, A J Craven, A G Cullis, N Daneu, V Grillo, E Grünbaum, A Gustafsson, P-H Jouneau, O L Krivanek, D Larson, M Luysberg, S I Molina, F A Ponce, A Rosenauer, F M Ross, I M Ross, J-L Rouviere, and Z L Wang. Prizes for student presentations at the conference were awarded to Mr Alberto Eljarrat, Universitat de Barcelona, and Mr Thibaud Denneulin, CEA-LETI Grenoble, both of whom are presenting their results also as contributions to this proceedings volume. Entertainment during the conference dinner was provided by the college's string quartet, and after the meal we held the now traditional friendly competition between teams of microscopists; this year teams were asked to construct models of scientific instruments using only cardboard, paper, sticky tape and glue. By unanimous vote, the team who presented the 'Atomic Food Microscope (AFM)' prototype won first prize - the deft 'dipping mode' of the cardboard cantilever (see photo) was a highlight! Figure 2 Figure 2. The prize winning 'Atomic Food Microscope (AFM)' in action! The organisers are very grateful to the following companies who contributed to the success of the meeting by presenting trade stands during an exhibition on the evening of 5 April: Agar Scientific, Bruker, CamScan, FEI, Gatan, Hitachi High Technologies, HREM Research, IOP Publishing, JEOL, Leica Microsystems and MICOS. Finally, we would like to thank the staff of the Institute of Physics for their expert assistance in planning and organising this conference and in particular Claire Garland for her dedicated professional support and her always joyful approach to any issues that arose. October 2011 T Walther P A Midgley

  18. Using a Remotely Piloted Aircraft System (RPAS) to analyze the stability of a natural rock slope

    Science.gov (United States)

    Salvini, Riccardo; Esposito, Giuseppe; Mastrorocco, Giovanni; Seddaiu, Marcello

    2016-04-01

    This paper describes the application of a rotary wing RPAS for monitoring the stability of a natural rock slope in the municipality of Vecchiano (Pisa, Italy). The slope under investigation is approximately oriented NNW-SSE and has a length of about 320 m; elevation ranges from about 7 to 80 m a.s.l.. The hill consists of stratified limestone, somewhere densely fractured, with dip direction predominantly oriented in a normal way respect to the slope. Fracture traces are present in variable lengths, from decimetre to metre, and penetrate inward the rock versant with thickness difficult to estimate, often exceeding one meter in depth. The intersection between different fracture systems and the slope surface generates rocky blocks and wedges of variable size that may be subject to phenomena of gravitational instability (with reference to the variation of hydraulic and dynamic conditions). Geometrical and structural info about the rock mass, necessary to perform the analysis of the slope stability, were obtained in this work from geo-referenced 3D point clouds acquired using photogrammetric and laser scanning techniques. In particular, a terrestrial laser scanning was carried out from two different point of view using a Leica Scanstation2. The laser survey created many shadows in the data due to the presence of vegetation in the lower parts of the slope and limiting the feasibility of geo-structural survey. To overcome such a limitation, we utilized a rotary wing Aibotix Aibot X6 RPAS geared with a Nikon D3200 camera. The drone flights were executed in manual modality and the images were acquired, according to the characteristics of the outcrops, under different acquisition angles. Furthermore, photos were captured very close to the versant (a few meters), allowing to produce a dense 3D point cloud (about 80 Ma points) by the image processing. A topographic survey was carried out in order to guarantee the necessary spatial accuracy to the process of images exterior

  19. Geomaterials and architecture of the medieval monuments of Sardinia (Italy): petrophysical investigations on their construction materials and documentation on the architectonic aspects using digital technologies

    Science.gov (United States)

    Columbu, Stefano; Verdiani, Giorgio

    2015-04-01

    The Sardinia Island is in the core area of the Mediterranean Sea. Its position has made it the crossing point of many cultural and political events, but at the same time its isolation has favoured the manifestation of specific and unique Cultural Heritage phenomena. The network of several medieval monuments (i.e., Romanesque churches) disseminated all around the island clearly shows how an architectural language can be declined according to site specific materials and specific artistical and practical choices, always preserving its original logic and grammar. On the bases of different architectural characteristics and petrophysical features of their lithology, a significant number of churches have been chosen from the different medieval geographical-political areas of the Sardinia named (at that time) "Giudicati". Each of these churches were surveyed using the following methods: photography, 3D Laser Scanner for the whole interior and exterior parts (using a Leica HDS 6000 and a Cam/2 Faro Photon units), photogrammetry (using high resolution Nikon D700 and D800e) of a selected set of the extern surface of significant altered samples (aimed to the production of high quality and highly detailed 3D digital models), direct sampling of representative rocks and ancient mortars for geochemical and minero-petrographic analysis using optical polarized microscope, electronic microscopy (SEM), X-Ray fluorescence (XRF), X-Ray diffractometry (XRD). The physical-mechanical properties (real and bulk densities, open and closed porosity, water absorption and saturation, vapour permeability, flexion and compression strengths, etc.) of various geomaterials are determined with helium picnometry, microscopic image analysis, gas-permeability thermostatic chamber, oil-hydraulic press machine, Point Load Test (PLT), abrasimeter. For each church, when there was the occasion, some specific case study has been developed, matching the information about the materials and the specific events

  20. OPEN SOURCE WEB TOOL FOR TRACKING IN A LOWCOST MOBILE MAPPING SYSTEM

    Directory of Open Access Journals (Sweden)

    F. Fissore

    2017-11-01

    Full Text Available During the last decade several Mobile Mapping Systems (MMSs, i.e. systems able to acquire efficiently three dimensional data using moving sensors (Guarnieri et al., 2008, Schwarz and El-Sheimy, 2004, have been developed. Research and commercial products have been implemented on terrestrial, aerial and marine platforms, and even on human-carried equipment, e.g. backpack (Lo et al., 2015, Nex and Remondino, 2014, Ellum and El-Sheimy, 2002, Leica Pegasus backpack, 2016, Masiero et al., 2017, Fissore et al., 2018. Such systems are composed of an integrated array of time-synchronised navigation sensors and imaging sensors mounted on a mobile platform (Puente et al., 2013, Tao and Li, 2007. Usually the MMS implies integration of different types of sensors, such as GNSS, IMU, video camera and/or laser scanners that allow accurate and quick mapping (Li, 1997, Petrie, 2010, Tao, 2000. The typical requirement of high-accuracy 3D georeferenced reconstruction often makes such systems quite expensive. Indeed, at time of writing most of the terrestrial MMSs on the market have a cost usually greater than 50000, which might be expensive for certain applications (Ellum and El-Sheimy, 2002, Piras et al., 2008. In order to allow best performance sensors have to be properly calibrated (Dong et al., 2007, Ellum and El-Sheimy, 2002. Sensors in MMSs are usually integrated and managed through a dedicated software, which is developed ad hoc for the devices mounted on the mobile platform and hence tailored for the specific used sensors. Despite the fact that commercial solutions are complete, very specific and particularly related to the typology of survey, their price is a factor that restricts the number of users and the possible interested sectors. This paper describes a (relatively low cost terrestrial Mobile Mapping System developed at the University of Padua (TESAF, Department of Land Environment Agriculture and Forestry by the research team in CIRGEO, in order to

  1. Comparative morpho-anatomical studies of the lesions caused by citrus leprosis virus on sweet orange

    Directory of Open Access Journals (Sweden)

    João P.R. Marques

    2010-06-01

    Full Text Available The leprosis disease shows a viral etiology and the citrus leprosis virus is considered its etiologic agent. The disease may show two types of cytopatologic symptom caused by two virus: nuclear (CiLV-N and cytoplasmic (CiLV-C types. The aim of this study was to compare the morpho-anatomical differences in the lesions caused by leprosis virus-cytoplasmic and nuclear types in Citrus sinensis (L. Osbeck 'Pêra'. Leaf and fruit lesions were collected in Piracicaba/São Paulo (cytoplasmic type and Monte Alegre do Sul/São Paulo and Amparo/São Paulo (nuclear type. The lesions were photographed and then fixed in Karnovsky solution, dehydrated in a graded ethylic series, embedded in hydroxy-ethyl methacrylate resin (Leica Historesin, sectioned (5 μm thick, stained and mounted in synthetic resin. The digital images were acquired in a microscope with digital video camera. Leaf and fruit lesions caused by the two viruses were morphologically distinct. Only the lesion caused by CiLV-N virus presented three well-defined regions. In both lesions there was the accumulation of lipidic substances in necrotic areas that were surrounded by cells with amorphous or droplets protein. Only leaf and fruit lesions caused by CiLV-N virus exhibited traumatic gum ducts in the vascular bundles.A doença leprose dos citros tem etiologia viral sendo o citrus leprosis virus seu agente etiológico. Demonstrou-se que há dois vírus distintos que causam sintomas de leprose em ci-tros: citoplasmático (CiLV-C e o nuclear (CiLV-N. O objetivo desse estudo foi comparar as diferenças morfo-anatômicas nas lesões causadas por CiLV-C e por CiLV-N em laranjeira doce (Citrus sinensis (L. Osbeck 'Pêra'. As lesões foliares e dos frutos foram coletadas em Piracicaba/SP (tipo citoplas-mático e em Monte Alegre do Sul/SP e Amparo/SP (tipo nuclear. As lesões foram fotografadas e em seguida fixadas em solução Karnovsky, desidratadas em série etílica, incluídas em historesina e

  2. The effect of space microgravity on the physiological activity of mammalian resident cardiac stem cells

    Science.gov (United States)

    Belostotskaya, Galina; Zakharov, Eugeny

    Prolonged exposure to weightlessness during space flights is known to cause depression of heart function in mammals. The decrease in heart weight and its remodeling under the influence of prolonged weightlessness (or space microgravity) is assumed to be due to both morphological changes of working cardiomyocytes and their progressive loss, as well as to possible depletion of resident cardiac stem cells (CSCs) population, or their inability to self-renewal and regeneration of muscle tissue under conditions of weightlessness. We have previously shown that the presence of different maturity clones formed by resident CSCs not only in culture but also in the mammalian myocardium can be used as an indicator of the regenerative activity of myocardial cells [Belostotskaya, et al., 2013: 2014]. In this study, we were interested to investigate whether the 30-day near-Earth space flight on the spacecraft BION-M1 affects the regenerative potential of resident CSCs. Immediately after landing of the spacecraft, we had examined the presence of resident c-kit+, Sca-1+ and Isl1+ CSCs and their development in suspension of freshly isolated myocardial cells of C57BL mice in comparison to controls. Cardiac cell suspension was obtained by enzymatic digestion of the heart [Belostotskaya and Golovanova, 2014]. Immunocytochemically stained preparations of fixed cells were analyzed with confocal microscope Leica TCS SP5 (Germany) in the Resource Center of St-Petersburg State University. CSCs were labeled with appropriate antibodies. CSCs differentiation into mature cardiomyocytes was verified using antibodies to Sarcomeric α-Actinin and Cardiac Troponin T. Antibodies to Connexin43 were used to detect cell-cell contacts. All antibodies were conjugated with Alexa fluorochromes (488, 532, 546, 568, 594 and/or 647 nm), according to Zenon-technology (Invitrogen). It has been shown that, under identical conditions of cell isolation, more complete digestion of heart muscle was observed in

  3. ALK Protein Analysis by IHC Staining after Recent Regulatory Changes: A Comparison of Two Widely Used Approaches, Revision of the Literature, and a New Testing Algorithm.

    Science.gov (United States)

    Marchetti, Antonio; Di Lorito, Alessia; Pace, Maria Vittoria; Iezzi, Manuela; Felicioni, Lara; D'Antuono, Tommaso; Filice, Giampaolo; Guetti, Luigi; Mucilli, Felice; Buttitta, Fiamma

    2016-04-01

    Recent regulatory changes have allowed the diagnostic use of immunohistochemical (IHC) analysis for the identification of patients with non-small cell lung cancer who are eligible for treatment with anaplastic lymphoma receptor tyrosine kinase (ALK) inhibitors. The U.S. Food and Drug Administration has approved the VENTANA ALK (D5F3) CDx Assay (Ventana Medical Systems, Tucson, AZ) as companion diagnostics, and the Italian Medicines Agency has recognized IHC analysis as a diagnostic test indicating an algorithm for patient selection. On the basis of the new regulations, we compared two commonly used IHC assays on 1031 lung adenocarcinomas: the VENTANA ALK (D5F3) CDx Assay with the OptiView Amplification Kit (Ventana Medical Systems) and a standard IHC test with the clone 5A4 (Novocastra, Leica Biosystems, Newcastle Upon Tyne, United Kingdom) along with their interpretative algorithms. Fluorescence in situ hybridization (FISH) was performed in all cases. Next-generation sequencing was performed in FISH/IHC analysis-discordant samples. FISH gave positive results in 33 (3.2%) cases. When FISH was used as a reference, the VENTANA ALK (D5F3) CDx assay had a sensitivity of 90.9% ± 2.6%, a specificity of 99.8% ± 0.6%, and positive and negative predictive values of 93.8% ± 2.1% and 99.7% ± 0.6%, respectively. The clone 5A4-based IHC test showed a sensitivity of 90.9% ± 2.6%, a specificity of 98.3% ± 1.3%, and positive and negative predictive values of 63.8% ± 4.2% and 99.7% ± 0.6%, respectively. Five cases with IHC analysis/FISH-discordant results in our series were analyzed together with those previously reported in the literature. Overall, data from 35 patients indicate a response rate to ALK inhibitors in 100% of FISH-negative/IHC analysis-positive cases (seven of seven) and 46% of FISH-positive/IHC analysis-negative cases (13 of 28), respectively. Our results confirm the difficulty in managing an IHC test without amplification in the absence of

  4. Open Source Web Tool for Tracking in a Lowcost Mobile Mapping System

    Science.gov (United States)

    Fissore, F.; Pirotti, F.; Vettore, A.

    2017-11-01

    During the last decade several Mobile Mapping Systems (MMSs), i.e. systems able to acquire efficiently three dimensional data using moving sensors (Guarnieri et al., 2008, Schwarz and El-Sheimy, 2004), have been developed. Research and commercial products have been implemented on terrestrial, aerial and marine platforms, and even on human-carried equipment, e.g. backpack (Lo et al., 2015, Nex and Remondino, 2014, Ellum and El-Sheimy, 2002, Leica Pegasus backpack, 2016, Masiero et al., 2017, Fissore et al., 2018). Such systems are composed of an integrated array of time-synchronised navigation sensors and imaging sensors mounted on a mobile platform (Puente et al., 2013, Tao and Li, 2007). Usually the MMS implies integration of different types of sensors, such as GNSS, IMU, video camera and/or laser scanners that allow accurate and quick mapping (Li, 1997, Petrie, 2010, Tao, 2000). The typical requirement of high-accuracy 3D georeferenced reconstruction often makes such systems quite expensive. Indeed, at time of writing most of the terrestrial MMSs on the market have a cost usually greater than 50000, which might be expensive for certain applications (Ellum and El-Sheimy, 2002, Piras et al., 2008). In order to allow best performance sensors have to be properly calibrated (Dong et al., 2007, Ellum and El-Sheimy, 2002). Sensors in MMSs are usually integrated and managed through a dedicated software, which is developed ad hoc for the devices mounted on the mobile platform and hence tailored for the specific used sensors. Despite the fact that commercial solutions are complete, very specific and particularly related to the typology of survey, their price is a factor that restricts the number of users and the possible interested sectors. This paper describes a (relatively low cost) terrestrial Mobile Mapping System developed at the University of Padua (TESAF, Department of Land Environment Agriculture and Forestry) by the research team in CIRGEO, in order to test an

  5. First test results of the airborne dispersive pushbroom imaging spectrometer APEX

    Science.gov (United States)

    Meuleman, K.; Itten, K.; Schaepman, M.

    2009-04-01

    APEX, ESA-Prodex "Airborne Prism Experiment" comprises the development of an airborne dispersive pushbroom imaging spectrometer and has originally been designed as flexible hyperspectral mission simulator and calibrator for existing and upcoming or planned future space missions. The APEX project is co-funded by Switzerland and Belgium and built by a Belgian-Swiss industrial team under the prime RUAG Aerospace (CH), responsible for the total system and the mechanical components, OIP (Oudenaarde, BE) contributing the spectrometer, and Netcetera (Zurich, CH) being responsible for the electronics. RSL (University of Zurich, CH) acts as scientific PI together with the Co-PI VITO (Mol, BE). The APEX sensor is operating between 380 nm and 2500 nm in more than 300 freely configurable bands (up to 512 bands in full spectral mode), by means of two dispersive spectrometer channels. 1000 pixels across track and a total field of view of 28° define the ground pixel size (e.g. 2,5 m from 5000 m AGL). A stabilized platform (Leica PAV-30) reduces major geometric distortions due to aircraft instabilities while a GPS/IMU system (Applanix PosAV 410) measures continuously the sensors' position and orientation allowing direct georeferencing of the acquired data . The system is currently is phase D, the calibration and test phase, and first testflights have been performed on a Do-228 in cooperation of DLR while the acquired data is currently under evaluation. Discussions are ongoing to fly APEX on the new DLR High Altitude Research Aircraft (HALO) as well. The system is currently in phase D, the calibration and test phase, and will deliver first scientific data to users by mid 2009. The APEX processing and archiving facility (PAF) is hosted by VITO in the APEX Operations Center (AOC) at Mol, Belgium . A specific level 0-1 processing software module producing uniform, radiometrically calibrated data has been developed by RSL and is integrated into the PAF by VITO. An APEX Calibration

  6. Analyzing RCD30 Oblique Performance in a Production Environment

    Science.gov (United States)

    Soler, M. E.; Kornus, W.; Magariños, A.; Pla, M.

    2016-06-01

    In 2014 the Institut Cartogràfic i Geològic de Catalunya (ICGC) decided to incorporate digital oblique imagery in its portfolio in response to the growing demand for this product. The reason can be attributed to its useful applications in a wide variety of fields and, most recently, to an increasing interest in 3d modeling. The selection phase for a digital oblique camera led to the purchase of the Leica RCD30 Oblique system, an 80MPixel multispectral medium-format camera which consists of one Nadir camera and four oblique viewing cameras acquiring images at an off-Nadir angle of 35º. The system also has a multi-directional motion compensation on-board system to deliver the highest image quality. The emergence of airborne oblique cameras has run in parallel to the inclusion of computer vision algorithms into the traditional photogrammetric workflows. Such algorithms rely on having multiple views of the same area of interest and take advantage of the image redundancy for automatic feature extraction. The multiview capability is highly fostered by the use of oblique systems which capture simultaneously different points of view for each camera shot. Different companies and NMAs have started pilot projects to assess the capabilities of the 3D mesh that can be obtained using correlation techniques. Beyond a software prototyping phase, and taking into account the currently immature state of several components of the oblique imagery workflow, the ICGC has focused on deploying a real production environment with special interest on matching the performance and quality of the existing production lines based on classical Nadir images. This paper introduces different test scenarios and layouts to analyze the impact of different variables on the geometric and radiometric performance. Different variables such as flight altitude, side and forward overlap and ground control point measurements and location have been considered for the evaluation of aerial triangulation and

  7. Calcareous nannofossil biostratigraphy of Sarcheshmeh and Sanganeh formations at Qaleh Jegh and Yazdan Abad sections (West of Kopet-Dagh Basin

    Directory of Open Access Journals (Sweden)

    Azam Gholami Fard

    2017-03-01

    calcareous marl, marl and argillaceous limestone in upper part. A fossiliferous limestone separates Sarcheshmeh and Sanganeh formations. Sanganeh Formation is 485 m thick comprising siltstones with sandstone, marl (with argillaceous limestone or limestone intercalation. A total of 1286 samples (1006 from Qaleh Jegh section and 280 from the Yazdan Abad section were collected at about 4 m intervals. For the purpose of biostratigraphic investigations, all of the samples were processed using the gravity settling technique (Bown and Young, 1998 and the prepared slides were observed under a Leica DMLP Pol light microscope at ×1000 magnification.   3-    Discussion of Results & Conclusions Nannofossil bioevents have been utilized to biostratigraphically classify the exposed sedimentary succession based on schemes of Roth (1978; modified by Bralower et al., 1993 and Sissingh (1977; modified by Perch-Nielsen, 1985. Overall, 56 species from 28 genera of 14 families in Qaleh Jegh section, and 45 species from 26 genera of 13 families in Yazdan Abad section have been identified and their distribution recorded. Based on the index calcareous nannofossil taxa, the upper part of NC6, NC7A, NC7 (B&C and lower part of NC8 nannofossil zones are delineated in Ghaleh Jegh and the upper part of NC5, NC6, NC7B and lower part of NC7A are recorded in Yazdan Abad. The distributions point to Early to Late Aptian time of deposition for the rocks of Sarcheshmeh Formation and Late Aptian to Early Albian for those of Sanganeh Formation in Ghaleh Jegh, while At Yazdan Abad, Sarcheshmeh Formation ranges from Late Barremian to Early Aptian and Sanganeh Formation dates Early to Late Aptian.

  8. Photogrammetric Metrology for the James Webb Space Telescope Integrated Science Instrument Module

    Science.gov (United States)

    Nowak, Maria; Crane, Allen; Davila, Pam; Eichhorn, William; Gill, James; Herrera, Acey; Hill, Michael; Hylan, Jason; Jetten, Mark; Marsh, James; hide

    2007-01-01

    The James Webb Space Telescope (JWST) is a 6.6m diameter, segmented, deployable telescope for cryogenic IR space astronomy (approximately 40K). The JWST Observatory architecture includes the Optical Telescope Element and the Integrated Science Instrument Module (ISIM) element that contains four science instruments (SI) including a Guider. The ISM optical metering structure is a roughly 2.2x1.7x2.2m, asymmetric frame that is composed of carbon fiber and resin tubes bonded to invar end fittings and composite gussets and clips. The structure supports the SIs, isolates the SIs from the OTE, and supports thermal and electrical subsystems. The structure is attached to the OTE structure via strut-like kinematic mounts. The ISIM structure must meet its requirements at the approximately 40K cryogenic operating temperature. The SIs are aligned to the structure's coordinate system under ambient, clean room conditions using laser tracker and theodolite metrology. The ISIM structure is thermally cycled for stress relief and in order to measure temperature-induced mechanical, structural changes. These ambient-to-cryogenic changes in the alignment of SI and OTE-related interfaces are an important component in the JWST Observatory alignment plan and must be verified. We report on the planning for and preliminary testing of a cryogenic metrology system for ISIM based on photogrammetry. Photogrammetry is the measurement of the location of custom targets via triangulation using images obtained at a suite of digital camera locations and orientations. We describe metrology system requirements, plans, and ambient photogrammetric measurements of a mock-up of the ISIM structure to design targeting and obtain resolution estimates. We compare these measurements with those taken from a well known ambient metrology system, namely, the Leica laser tracker system. We also describe the data reduction algorithm planned to interpret cryogenic data from the Flight structure. Photogrammetry was

  9. Vegetation Structure, Tree Volume and Biomass Estimation using Terrestrial Laser Scanning Remote Sensing: A Case Study of the Mangrove Forests in the Everglades National Park

    Science.gov (United States)

    Feliciano, E. A.; Wdowinski, S.; Potts, M. D.

    2012-12-01

    Mangrove forests are being threatened by accelerated climate change, sea level rise and coastal projects. Carbon/above ground biomass (AGB) losses due to natural or human intervention can affect global warming. Thus, it is important to monitor AGB fluctuations in mangrove forests similar to those inhabiting the Everglades National Park (ENP). Tree volume and tree wood specific density are two important measurements for the estimation of AGB (mass = volume * density). Wood specific density is acquired in the laboratory by analyzing stem cores acquired in the field. However, tree volume is a challenging task because trees resemble tapered surfaces. The majority of published studies estimate tree volume and biomass using allometric equations, which describe the size, shape, volume or AGB of a given population of trees. However, these equations can be extremely general and might not give a representative value of volume or AGB for a specific tree species. In order to have precise biomass estimations, other methodologies for tree volume estimation are needed. To overcome this problem, we use a state-of-the-art remote sensing tool known as ground-based LiDAR a.k.a Terrestrial Laser Scanner (TLS), which can be used to precisely measure vegetation structure and tree volume from its 3-D point cloud. We surveyed three mangrove communities: (Rhizophora mangle, Laguncuria racemosa and Avicennia germinans) in three different sites along Shark River Slough (SRS), which is the primary source of water to the ENP. Our sites included: small-, intermediate- and tall- size mangroves. Our ground measurements included both: traditional forestry surveys and TLS surveys for tree attributes (tree height and diameter at breast height (DBH)) comparison. These attributes are used as input to allometric equations for the estimation of tree volume and AGB. A total of 25 scans were collected in 2011 with a Leica ScanStation C10 TLS. The 3-D point cloud acquired from the TLS data revealed that

  10. Importance of Laser Scanning Resolution in the Process of Recreating the Architectural Details of Historical Buildings

    Science.gov (United States)

    Pawłowicz, Joanna A.

    2017-10-01

    The TLS method (Terrestrial Laser Scanning) may replace the traditional building survey methods, e.g. those requiring the use measuring tapes or range finders. This technology allows for collecting digital data in the form of a point cloud, which can be used to create a 3D model of a building. In addition, it allows for collecting data with an incredible precision, which translates into the possibility to reproduce all architectural features of a building. This data is applied in reverse engineering to create a 3D model of an object existing in a physical space. This study presents the results of a research carried out using a point cloud to recreate the architectural features of a historical building with the application of reverse engineering. The research was conducted on a two-storey residential building with a basement and an attic. Out of the building’s façade sticks a veranda featuring a complicated, wooden structure. The measurements were taken at the medium and the highest resolution using a ScanStation C10 laser scanner by Leica. The data obtained was processed using specialist software, which allowed for the application of reverse engineering, especially for reproducing the sculpted details of the veranda. Following digitization, all redundant data was removed from the point cloud and the cloud was subjected to modelling. For testing purposes, a selected part of the veranda was modelled by means of two methods: surface matching and Triangulated Irregular Network. Both modelling methods were applied in the case of data collected at medium and the highest resolution. Creating a model based on data obtained at medium resolution, both by means of the surface matching and the TIN method, does not allow for a precise recreation of architectural details. The study presents certain sculpted elements recreated based on the highest resolution data with superimposed TIN juxtaposed against a digital image. The resulting model is very precise. Creating good models

  11. The clinical and morphological aspects of aetiology and pathogenesis of sacrococcygeal pilonidal cysts

    Directory of Open Access Journals (Sweden)

    Ye. V. Tsema

    2013-12-01

    Full Text Available Introduction. The occurrence of pilonidal cysts in Ukrainian population is up to 50 cases per 100.000. Nevertheless, the cause and the pathogenesis of pilonidal cyst to date remain unclear. There are two opposite views on the etiology of the pilonidal disease stating it has congenital or acquired origin. Authors suggest the definite answer should be based primarily on the results of the morphologic evaluation of pilonidal cyst tissue. Aim: To explore the morphological features of sacrococcygeal pilonidal cysts by means of histological evaluation of cyst tissue after the wide local excision of the cyst. Methods: We performed complex morphological evaluation of cyst tissue obtained after the wide local excision of pilonidal cyst to find out particulars of sacrococcygeal pilonidal cyst histological structure. In total, we evaluated 42 surgical specimens obtained after the wide local excision of pilonidal cyst complicated by the secondary sinus tract formation. The microscopy was performed with the light microscope Leica DM LS2 (ocular lens: х10, objective lens х10 or х20, camera’s optical zoom х4. Histological samples were stained with hematoxylin and eosin using the standard method. Discussion. The absence of own epithelial elements in the pilonidal cysts and the secondary sinus tracts have been demonstrated. Hypertrophic growth of skin or hair follicle epithelium was evident in some specimens. Such changes seen in the deep layers of skin on the border with adipose tissue were similar to epidermal polyps. Results. There are some morphological features suggesting the acquired origin of the pilonidal disease as follows: - Hair found in the pilonidal cyst’s tissue is not associated with hair follicles, and occurs as loose shafts with atrophied hair bulb, and their exogenous transdermal penetration is evident. - Pilonidal cyst doesn’t have own epithelium, and the epithelial fragments that occur are the fragments of disorganized hair

  12. Evaluating the accuracy of low cost UAV generated topography and its effectiveness for geomorphic change detection

    Science.gov (United States)

    Cook, Kristen

    2015-04-01

    With the recent explosion in the use and availability of unmanned aerial vehicle platforms and development of easy to use structure from motion (SfM) software, UAV based photogrammetry is increasingly being adopted to produce high resolution topography for the study of surface processes. UAV systems can vary substantially in price and complexity, but the tradeoffs between these and the quality of the resulting data are not well constrained. We look at one end of this spectrum and evaluate the effectiveness of a simple low cost UAV setup for obtaining high resolution topography in a challenging field setting. Our study site is the Daan River gorge in western Taiwan, a rapidly eroding bedrock gorge that we have monitored with terrestrial Lidar since 2009. The site presents challenges for the generation and analysis of high resolution topography, including vertical gorge walls, vegetation, wide variation in surface roughness, and a complicated 3D morphology. In order to evaluate the accuracy of the UAV-derived topography, we compare it with terrestrial Lidar data collected during the same survey period. Our UAV setup combines a DJI Phantom 2 quadcopter with a 16 megapixel Canon Powershot camera for a total platform cost of less than 850. The quadcopter is flown manually, and the camera is programmed to take a photograph every 4 seconds, yielding 200-250 pictures per flight. We measured ground control points and targets for both the Lidar scans and the aerial surveys using a Leica RTK GPS with 1-2 cm accuracy. UAV derived point clouds were obtained using Agisoft Photoscan software. We conducted both Lidar and UAV surveys before and after the 2014 typhoon season, allowing us to evaluate the reliability of the UAV survey to detect geomorphic changes in the range of one to several meters. The accuracy of the SfM point clouds depends strongly on the characteristics of the surface being considered, with vegetation and small scale texture causing inaccuracies. However, we

  13. Development of a Whole Slide Imaging System on Smartphones and Evaluation With Frozen Section Samples.

    Science.gov (United States)

    Yu, Hong; Gao, Feng; Jiang, Liren; Ma, Shuoxin

    2017-09-15

    The aim was to develop scalable Whole Slide Imaging (sWSI), a WSI system based on mainstream smartphones coupled with regular optical microscopes. This ultra-low-cost solution should offer diagnostic-ready imaging quality on par with standalone scanners, supporting both oil and dry objective lenses of different magnifications, and reasonably high throughput. These performance metrics should be evaluated by expert pathologists and match those of high-end scanners. The aim was to develop scalable Whole Slide Imaging (sWSI), a whole slide imaging system based on smartphones coupled with optical microscopes. This ultra-low-cost solution should offer diagnostic-ready imaging quality on par with standalone scanners, supporting both oil and dry object lens of different magnification. All performance metrics should be evaluated by expert pathologists and match those of high-end scanners. In the sWSI design, the digitization process is split asynchronously between light-weight clients on smartphones and powerful cloud servers. The client apps automatically capture FoVs at up to 12-megapixel resolution and process them in real-time to track the operation of users, then give instant feedback of guidance. The servers first restitch each pair of FoVs, then automatically correct the unknown nonlinear distortion introduced by the lens of the smartphone on the fly, based on pair-wise stitching, before finally combining all FoVs into one gigapixel VS for each scan. These VSs can be viewed using Internet browsers anywhere. In the evaluation experiment, 100 frozen section slides from patients randomly selected among in-patients of the participating hospital were scanned by both a high-end Leica scanner and sWSI. All VSs were examined by senior pathologists whose diagnoses were compared against those made using optical microscopy as ground truth to evaluate the image quality. The sWSI system is developed for both Android and iPhone smartphones and is currently being offered to the

  14. A prostaglandin E2 receptor antagonist prevents pregnancies during a preclinical contraceptive trial with female macaques.

    Science.gov (United States)

    Peluffo, M C; Stanley, J; Braeuer, N; Rotgeri, A; Fritzemeier, K-H; Fuhrmann, U; Buchmann, B; Adevai, T; Murphy, M J; Zelinski, M B; Lindenthal, B; Hennebold, J D; Stouffer, R L

    2014-07-01

    and blood lipid profiles. Several mRNAs encoding proteins involved in PGE2 synthesis, metabolism and signaling increase (P contraceptive effect without altering steroid hormone patterns or menstrual cyclicity during a 5-months contraceptive trial. Fertility recovered as early as 1 month after ending treatment. This is a proof-of-concept study in a non-human primate model. Further investigations are warranted to elucidate the mechanism(s) of PTGER2 antagonist action in the primate ovary. Although PTGER2 antagonist treatment did not produce any obvious undesirable effects, improvements in the mode of administration, as well as the efficacy of these compounds, are necessary to consider such a contraceptive for women. Monitoring as well as improving the efficacy and safety of female contraceptives is an important public health activity. Even though hormonal contraceptives are effective for women, concerns remain regarding their side-effects and long-term use because of the widespread actions of such steroidal products in many tissues. Moreover, some women cannot take hormones for medical reasons. Thus, development of non-hormonal contraceptives for women is warranted. Supported by Bayer HealthCare Pharmaceuticals, The Eunice Kennedy Shriver NICHD Contraceptive Development and Research Center (U54 HD055744), NIH Office of the Director (Oregon National Primate Research Center P51 OD011092), and a Lalor Foundation Postdoctoral Basic Research Fellowship (MCP). The use of the Leica confocal was supported by grant number S10RR024585. Some of the authors (N.B., A.R., K.-H.F., U.F., B.B. and B.L.) are employees of Bayer Healthcare Pharma.

  15. A mobile GPR system for evaluating road landslide damage and effectiveness of the restoration works at Potenza District scale

    Science.gov (United States)

    Loperte, A.; Bavusi, M.; Soldovieri, F.

    2012-04-01

    Basilicata Region (Southern Italy) is affected by a large amount of landslides due to its geological structure and orogenic history. In particular, due to its location in the Italian Apennine chain, the District of Potenza is particularly affected by this natural hazard, which threatens private buildings and public infrastructures. In this framework, the transport infrastructures result greatly exposed to this kind of damage since there is a great probability that a road intercepts a landslide in its path. In addition, this can have relevant "social and economic" effects at large distance from the landslide due to the interconnected character of the road network. A classical remediation strategy for a damaged road, especially when affected by a localised landslide, consists in restoring its planarity by filling the depression induced by the damage. Moreover a such intervention often causes the recurrence of the damage since it results in a weakness zone in the road section. The result is that there is always an interrupted road somewhere in Basilicata. In order to design an effective remediation strategy at the scale of the district road network, a fast low-cost diagnosis method is needed in order to: • Detect damaged/restored treats of roads; • Characterize previous restoration intervention quality; • Provide a geo-database reporting all at-risk road sections; • Predict which road sections might be damaged with the upcoming rainfall events. In this framework, the Institute of Methodologies for Environmental Analysis (IMAA-CNR), also thanks to the cooperation of the Institute for Electromagnetic Sensing of the Environment (IREA-CNR) has equipped a van with a high-resolution Ground Penetrating Radar and differential GPS instrumentations so to achieve a mobile GPR system. The main elements of the GPR are the control unit IDS DAD MCH Fast-Wave, horn antenna air-launched IDS HN-1000b and high speed encoder, differential GPS 1200 Leica. Main feature of this

  16. Integration of X-band SAR interferometry, continuous and periodic D-GPS and in-place inclinometers to characterize and monitor a deep-seated earthslide in the Dolomites (Italy)

    Science.gov (United States)

    Mulas, Marco; Corsini, Alessandro; Soldati, Mauro; Marcato, Gianluca; Pasuto, Alessandro; Crespi, Mattia; Mazzoni, Augusto; Benedetti, Elisa; Branzanti, Mara; Manunta, Michele; Ojha, Chandrakanta; Chinellato, Giulia; Cuozzo, Giovanni; Costa, Armin; Monsorno, Roberto; Thiebes, Benni; Piantelli, Elena; Magnani, Massimo; Meroni, Marco; Mair, Volkmar

    2015-04-01

    The Corvara landslide is an active, large-scale, deep-seated and slow moving earthslide of about 30 Mm3 located in the Dolomites (Italy). It is frequently damaging a national road and, occasionally, isolated buildings and recreational ski facilities. Since the mid '90s it has been mapped, dated and monitored thanks to field surveys, boreholes, radiocarbon dating, inclinometers, piezometers and periodic D-GPS measurements, carried out by the Geology and the Forestry Planning offices of the Autonomous Province of Bolzano, the Municipality of Corvara in Badia, the University of Modena and Reggio Emilia, the IRPI-CNR of Padua. In 2013, a new phase of characterization and monitoring has started which also involves the EURAC's Institute for Applied Remote Sensing, the geodesy group of University La Sapienza, the CNR-IREA of Naples and the Leica Geosystems office in Italy. This new phase of characterization and monitoring is meant to investigate the opportunities of innovative SAR interferometry, D-GPS and in-place inclinometers techniques to provide for a high frequency monitoring of the study site in support to the analysis of the investigation of forcing factors leading unsteady, nonuniform landslide motion through different seasons of the year. Monitoring results are also expected to provide a validation of innovative interferometric techniques so to fully evaluate their conformity to be used as a long-term monitoring system in land-use planning and risk management procedures. The monitoring infrastructure now integrates: 16 Corner Reflector for satellite X-Band SAR interferometric products, 13 benchmarks for D-GPS periodic surveys, three on-site GPS receivers for continuous positioning and remote ftp data pushing, two in-place inclinometers and a pressure transducer to record pore-pressure variations. The coupling of SAR-based products with GPS records is achieved using especially designed Corner Reflectors having an appendix dedicated to hold Dual-Frequency GPS

  17. ANALYZING RCD30 OBLIQUE PERFORMANCE IN A PRODUCTION ENVIRONMENT

    Directory of Open Access Journals (Sweden)

    M. E. Soler

    2016-06-01

    Full Text Available In 2014 the Institut Cartogràfic i Geològic de Catalunya (ICGC decided to incorporate digital oblique imagery in its portfolio in response to the growing demand for this product. The reason can be attributed to its useful applications in a wide variety of fields and, most recently, to an increasing interest in 3d modeling. The selection phase for a digital oblique camera led to the purchase of the Leica RCD30 Oblique system, an 80MPixel multispectral medium-format camera which consists of one Nadir camera and four oblique viewing cameras acquiring images at an off-Nadir angle of 35º. The system also has a multi-directional motion compensation on-board system to deliver the highest image quality. The emergence of airborne oblique cameras has run in parallel to the inclusion of computer vision algorithms into the traditional photogrammetric workflows. Such algorithms rely on having multiple views of the same area of interest and take advantage of the image redundancy for automatic feature extraction. The multiview capability is highly fostered by the use of oblique systems which capture simultaneously different points of view for each camera shot. Different companies and NMAs have started pilot projects to assess the capabilities of the 3D mesh that can be obtained using correlation techniques. Beyond a software prototyping phase, and taking into account the currently immature state of several components of the oblique imagery workflow, the ICGC has focused on deploying a real production environment with special interest on matching the performance and quality of the existing production lines based on classical Nadir images. This paper introduces different test scenarios and layouts to analyze the impact of different variables on the geometric and radiometric performance. Different variables such as flight altitude, side and forward overlap and ground control point measurements and location have been considered for the evaluation of aerial

  18. Quantification of Colloid Retention in Unsaturated Porous Media Using Microscopic Image Analysis Data

    Science.gov (United States)

    Dathe, A.; Zevi, Y.; Gao, B.; Richards, B. K.; Steenhuis, T. S.

    2006-05-01

    The movement of contaminants via colloidal transport mechanisms through the vadose zone to groundwater is of growing concern. Normally-immobile contaminants can enter an aquifer via colloid-facilitated transport, and pathogens themselves (e.g. Cryptosporidium parvum) are colloidal in scale. Little is known about the complex pore-scale mechanisms of transport and retention of colloids in soils. Measurements of colloid and microbial transport have been typically limited to the evaluation of breakthrough curves from column experiments (which yield only an integrated signal of all retention processes in the column) or to the visualization in micromodels with limited applicability to realistic conditions. The objective of the work discussed here is to observe and model colloid transport and retention on the pore scale. Flow experiments were run in a horizontal flow chamber containing clean quartz sand as the porous medium. Synthetic fluorescent microspheres were used as easily-detected colloid surrogates. A syringe inlet pump and peristaltic outlet pump controlled the chamber moisture content and flow rate. The chamber was mounted under a Laser Scanning Confocal Microscope (Leica TCS SP2, 10x 0.40 UV objective) which allowed the acquisition of time series images and 3D reconstruction of pore-scale images. Three spectral channels were used to detect: 1) fluorescent microsphere emissions (500 to 540 nm) excited at 488 nm by an argon laser; 2) water phase emissions (555 to 650 nm) due to Rhodamine B stain excited at 543 nm by a green HeNe laser; and 3) reflectance of laser light at the grain surfaces. Three 8-bit images were detected simultaneously for every time step. The system is also capable of obtaining image stacks in the z-direction, which allow the determination of the position of attached colloids relative to the interface between air, water menisci, and solid grains. The 3D z-axis stacks reveal that the colloids are attaching at the air/water meniscus/solid (AWm

  19. Chemical and microbiological evaluation of the internal surfaces of aluminum tubes both unlined and lined with epoxi resin by means of the stereoscope and scanning electron microscope Avaliação química e microbiológica da superfície interna dos tubos de alumínio não revestido e revestido com resina epóxi por meio de microscópio estereoscópio e eletrônico de varredura

    Directory of Open Access Journals (Sweden)

    Elisabeth Loshchagin Pizzolitto

    2001-12-01

    Full Text Available The internal surfaces of aluminum tubes, both unlined and lined with epoxy resin, which are used for packaged medicines such as creams, ointments, gels, etc., were evaluated chemically, and microbiologically with regard to microorganism adherence. Proof of porosity of the unlined aluminum surface and lined with epoxy resin, and the resistance to removal of the resin were observed using a scanning electron microscope and a stereoscope, with a digitalized image system. To evaluate the microorganisms adherence to the aluminum surface, unlined and lined with epoxy resin, sterilized disks (10mm in diameter were immersed in Mueller Hinton Broth and placed in tubes of polypropylene with screw caps. The liquid culture medium was then inoculated with suspensions (10(9CFU/ml of the following microorganisms: Streptococcus agalactiae, Staphylococcus aureus, Acinetobacter lwoffii and Candida albicans and incubated at 37°C under constant agitation (100rpm for 12 days. The culture broth was changed every 3 days, after which the disks were removed, and observed under the scanning electron microscope. Microscopic observations revealed adherence of the microorganisms and biofilm on the aluminum surfaces both unlined and lined with epoxy resin.A superfície interna das bisnagas fabricadas com alumínio não revestido e revestido com resina epóxi, utilizadas para acondicionar cremes, pomadas, géis, etc., foram avaliadas quimicamente e por métodos microbiológicos correlacionados com a aderência de microrganismos. A prova da porosidade e da resistência à remoção da resina foi observada por meio do microscópio eletrônico de varredura (Topcon FM300 e estereoscópio Leica (MZ12 acoplado a Sistema de Digitalização de Imagens. Para avaliar a ação dos microrganismos foram utilizados corpos-de-prova esterilizados (discos de 10mm de diâmetro, imersos em caldo Mueller Hinton (Difco e colocados em tubos de polipropileno com tampa de rosca (Corning. Foram

  20. AUTHOR GUIDELINES

    Directory of Open Access Journals (Sweden)

    Chief Editor

    2014-12-01

    :Articles that present innovations / best practicesAbstract: 100 wordsMain Text: 1500 wordsReferences: 10 (PubMed - Citation preferredTable / Figure: 1 – 2Continued Medical Education:In-depth, substantiated, educational articles presenting core information for the continuing professional development word count: 3500 words (excluding references, figures, and tablesLetters to the Editor:Raising issues on articles published in IJCHCommunications of about 500 – 800 words from readersBook reviews:Critical look at relevant booksMain Text: Up to 500 wordsCommon Interests:Conference Report, Minutes of Meeting, Audited Account Statement of Association & IJCH, Awards, Oration, Remembrance, etc.Editorial WorkflowNote: The IJCH strongly discourages duplication/reduplication of data already published in other journals (even when certain cosmetic changes/additions are made. If and when duplication is detected after publishing in IJCH, the journal will be forced to ‘retract’ such articles.  All papers submitted to IJCH are subject to peer review process. All accepted papers will be suitably edited before publication.Manuscript Handling Charges: (In Two Steps - w.e.f. 07.11.2014The journal charges fee on submission of Original Article, Review Article, Short Communication/Article, Report from the field, Continued Medical education, Letters to the Editor and is a mandatory prerequisite to start editorial screening. (To be deposited within one week of submission1.      Article Submission Fee:In case First Author is Members of IAPSM by NEFT/RTGS/Online Transfer/Net Banking - Submission Fee Rs.500/- and by Cash deposit in account - Rs.550/-.In case First Author is Non-Members by NEFT/RTGS/Online Transfer/Net Banking - Submission Fee Rs.1000/- and by Cash deposit in account - Rs.1050/-.International Author(s by PayPal - 20US$ Submission Fee (Seller e mail: alphacomputerdd@rediffmail.com2.      Article Processing Fee:In case First Author is Members of IAPSM by NEFT

  1. Restoration of Bhoj Wetlands At Bhopal, India

    Science.gov (United States)

    Shukla, S. S.; Kulshrestha, M.; Wetland Project, Bhoj

    financial savings as well as devel- opment of in-house expertise. The works have also been taken up manually as far as possible, giving employment opportunities to thousands of poor residents of Bhopal City and nearby villages. This also has ensured participation of masses by default. The project had at the time of its inception envisaged a sum of Rs. 2470 millions. As a result of manual pursuance of work, development of in-house expertise and due to various other cost reduction exercises adopted by the project authorities and engineers the project has so far spent only Rs. 1100 millions thus resulting in huge savings. A part of savings (Rs. 500 millions) has been invested to form a Lake Conservation Authority U a Government body which will look after all the lakes in the entire state 1 of Madhya Pradesh, thus contributing immensely to the cause of lake conservation. One of the special features of this project has been the involvement of masses in its various developmental schemes. This has been ensured by involving more than 55 NGOs and dozens of local educational institutions. Millions of Rupees have been ear- marked for ensuring public participation in various sub-projects. The project on its completion within the next 4 months will contribute immensely to the conservation of both the lakes which can them become role models for other similar wetlands. The paper also details out the various project works like diversion and treatment of nearly 56 MLD of sewage, reduction of harmful inflows of chemicals from activities such as washing activity, idol immersions etc., reduction measures for silt inflow to the lakes, desilting and dredging operations to increase the lake capacity by more than 2.5 M.Cum, improvement in the water quality by reduction of pollution, deweeding oper- ations, nutrient removal by dredging, increased aquaculture activities etc., large scale afforestation (1.6 million trees have been planted in the catchment) and implementa- tion of solid waste

  2. VADASE: a new approach for real-time fast displacement detection - First application to Taiwan High-Rate GNSS Network

    Science.gov (United States)

    Hung, Huang-Kai; Rau, Ruey-Juin; Colosimo, Gabriele; Benedetti, Elisa; Branzanti, Mara; Crespi, Mattia; Mazzoni, Augusto

    2014-05-01

    with very promising results to GPS single frequency data in occasion of the Emilia Earthquake (Italy, M=6.0 - May 20, 2012) [2] and also to Galileo single and dual frequency data [1]. VADASE, in principle, can be embedded into the receiver firmware and work in real-time. In this respect, at present, in the frame of an investigation project, VADASE has been implemented within the last release of the firmware of Leica Geosystems GR receivers. Here we present the application of the VADASE methodology to data collected at several permanent stations of Taiwan High-Rate GNSS Network in occasion of recent earthquakes occurred on June 2, 2013 (south-west of Buli, M=6.2, USGS) and October 31, 2013 (south-west of Hualian, M=6.3, USGS). Furthermore a solutions comparison with results obtained from co-located accelerometers and seismometers is shown and all the kinematic parameters are investigated. In particular, estimated velocities from VADASE are directly compared with seismometers dataset. Then, VADASE velocities are derived to perform a comparison with data collected by accelerometers. Finally, a comparison in terms of displacements obtained from all the instruments is carried out. With these tests a new strategy to integrate traditional seismic network with the information coming from GNSS data can be better assessed. [1] Benedetti E, Branzanti M, Colosimo G, Mazzoni A, Crespi M (2013). VADASE: state of the art and new developments of a third way to GNSS Seismology. International Association of Geodesy Symposia (Accepted for publication) [2] Benedetti E, Branzanti M, Biagi L, Colosimo G, Mazzoni A, Crespi M (2013). GNSS seismology for the 2012 Mw = 6.1 Emilia Earthquake: exploiting the VADASE algorithm. Seismological Research Letters (Accepted for publication) [3] Branzanti M, Colosimo G, Crespi M, Mazzoni A (2012) GPS near real-time coseismic displacements for the great Tohoku-Oki earthquake, doi:10.1109/LGRS.2012.2207704, IEEE Geoscience and Remote Sensing Letters [4

  3. Airborne & SAR Synergy Reveals the 3D Structure of Air Bubble Entrainment in Internal Waves and Frontal Zones

    Science.gov (United States)

    da Silva, J. C. B.; Magalhaes, J. M.; Batista, M.; Gostiaux, L.; Gerkema, T.; New, A. L.

    2013-03-01

    spectral range 8-12 μm. With a nominal ground resolution of approximately 1.5 meters (at an altitude of 500 meters) it is capable to detect fine structure associated to turbulence. The LiDAR system that has been used is the Leica ALS50-II (1064nm) with a hit rate greater than 1 hit per square meter and a vertical resolution of approximately 15 cm. Both systems were available simultaneously, together with the hyperspectral system and the RCD105 39Mpx digital camera, integrated with the LiDAR navigation system. We analyse the airborne data together with a comprehensive dataset of satellite Synthetic Aperture Radar (SAR) that includes ENVISAT and TerraSAR-X images. In addition, in situ observations in the near-shore zone were obtained in a previous experiment (Project SPOTIWAVE-II POCI/MAR/57836/2004 funded by the Portuguese FCT) during the summer period in 2006. These included thermistor chain measurements along the water column that captured the vertical structure of shoaling internal (tidal) waves and ISWs close to the breaking point. The SAR and airborne images were obtained in light wind conditions, in the near-shore zone, and in the presence of ISWs. The LiDAR images revealed sub-surface structures (some 1-2 m below the sea surface) that were co-located with surface films. These film slicks were induced by the convergent fields of internal waves and upwelling fronts. Some of the sub-surface features were located over the front slopes of the internal waves, which coincides with the internal wave slick band visible in the aerial photos and hyperspectral systems. Our flight measurements revealed thermal features similar to “boils” of cold water within the wake of (admittedly breaking) internal waves. These features are consistent with the previous in situ measurements of breaking ISWs. In this paper we will show coincident multi-sensor airborne and satellite SAR observations that reveal the 3D structure of air bubble entrainment in the internal wave field and frontal

  4. PREFACE: 18th Microscopy of Semiconducting Materials Conference (MSM XVIII)

    Science.gov (United States)

    Walther, T.; Hutchison, John L.

    2013-11-01

    are very grateful to the following companies who contributed to the success of the meeting by presenting trade stands on 9 and 10 April: Agar Scientific, Fischione, Gatan, ISS Group Services and Leica Microsystems. St Catherine's College is thanked for provision of excellent accommodation, superb lecture facilities and the very friendly catering staff who served food and drink during the days. There was even a compliment from some of our French visitors for the cheese board during the conference dinner! Finally, we would like to thank the staff of the Royal Microscopical Society for their expert help in planning and support of this conference - in particular Victoria Masters and her assistant Alice Pyper for their dedicated professional support and always joyful approach to any issues that arose. September 2013 Thomas Walther John L Hutchison

  5. Topographical, Geological and Geophysical Measurements in the Diamer Basha Dam Area (Gilgit-Balistan, Pakistan)

    Science.gov (United States)

    Poretti, Giorgio; Calligaris, Chiara; de Min, Gino; Tariq, Shahina; Kahn, Hawas; Zubair, Faqhia

    2010-05-01

    the whole length of the lake. The present study shows the preliminary results of an initial topographical survey of the dam's area realized within the framework of a larger monitoring project called "Nanga Parbat - Haramosh Massif Monitoring Project" the target of which is to determine the uplift of the massif due to geo-tectonic compressions. In fact northern Pakistan comprises three former distinct and previously separate plates named Karakoram, Kohistan island arc and Indian plates. They collided with each other during the Cretaceous-Tertiary ages and formed the present day configuration of this region (Himalayan orogeny) [3]. The project presented here was started in April of 2009 and developed through three different phases: a) Study of the area, contacts with the local authorities and identification of the points to be surveyed b) Monumentation of the benchmarks c) First measurement on the selected benchmarks. Since the location of the dam was very close to the Nanga Parbat-Haramosh massif It was decided to devote to it a special attention setting up a small topographical network. Three benchmarks were put on each bank (right and left) of the Indus river in order to measure the relative movements of the banks before and after the construction of the dam. The measurements were performed with a Leica TS30. Total Station following the scheme of the double parallelogram reproduced in Fig. 1 [3]. The measurements taken, before, during and after the construction of the dam will be a remarkable index of the change in the geophysical parameters produced by the dam and the related lake. References [1] http://www.paklinks.com/gs/pakistan-affairs/215216-president-musharraf-opens-work-diamer-basha-dam.html [2] http://www.defence.pk/forums/economy-development/12401-diamer-basha-dam-engineering-design-tender-documents-project-completed.html [3] http://www.dmi.units.it/~poretti/figureegubasha

  6. TEM Analyses of Itokawa Regolith Grains and Lunar Soil Grains to Directly Determine Space Weathering Rates on Airless Bodies

    Science.gov (United States)

    Berger, Eve L.; Keller, Lindsay P.; Christoffersen, Roy

    2016-01-01

    Samples returned from the moon and Asteroid Itokawa by NASA's Apollo Missions and JAXA's Hayabusa Mission, respectively, provide a unique record of their interaction with the space environment. Space weathering effects result from micrometeorite impact activity and interactions with the solar wind. While the effects of solar wind interactions, ion implantation and solar flare particle track accumulation, have been studied extensively, the rate at which these effects accumulate in samples on airless bodies has not been conclusively determined. Results of numerical modeling and experimental simulations do not converge with observations from natural samples. We measured track densities and rim thicknesses of three olivine grains from Itokawa and multiple olivine and anorthite grains from lunar soils of varying exposure ages. Samples were prepared for analysis using a Leica EM UC6 ultramicrotome and an FEI Quanta 3D dual beam focused ion beam scanning electron microscope (FIB-SEM). Transmission electron microscope (TEM) analyses were performed on the JEOL 2500SE 200kV field emission STEM. The solar wind damaged rims on lunar anorthite grains are amorphous, lack inclusions, and are compositionally similar to the host grain. The rim width increases as a smooth function of exposure age until it levels off at approximately 180 nm after approximately 20 My (Fig. 1). While solar wind ion damage can only accumulate while the grain is in a direct line of sight to the Sun, solar flare particles can penetrate to mm-depths. To assess whether the track density accurately predicts surface exposure, we measured the rim width and track density in olivine and anorthite from the surface of rock 64455, which was never buried and has a surface exposure age of 2 My based on isotopic measurements. The rim width from 64455 (60-70nm) plots within error of the well-defined trend for solar wind amorphized rims in Fig. 1. Measured solar flare track densities are accurately reflecting the

  7. Natural-color and color-infrared image mosaics of the Colorado River corridor in Arizona derived from the May 2009 airborne image collection

    Science.gov (United States)

    Davis, Philip A.

    2013-01-01

    The Grand Canyon Monitoring and Research Center (GCMRC) of the U.S. Geological Survey (USGS) periodically collects airborne image data for the Colorado River corridor within Arizona (fig. 1) to allow scientists to study the impacts of Glen Canyon Dam water release on the corridor’s natural and cultural resources. These data are collected from just above Glen Canyon Dam (in Lake Powell) down to the entrance of Lake Mead, for a total distance of 450 kilometers (km) and within a 500-meter (m) swath centered on the river’s mainstem and its seven main tributaries (fig. 1). The most recent airborne data collection in 2009 acquired image data in four wavelength bands (blue, green, red, and near infrared) at a spatial resolution of 20 centimeters (cm). The image collection used the latest model of the Leica ADS40 airborne digital sensor (the SH52), which uses a single optic for all four bands and collects and stores band radiance in 12-bits. Davis (2012) reported on the performance of the SH52 sensor and on the processing steps required to produce the nearly flawless four-band image mosaic (sectioned into map tiles) for the river corridor. The final image mosaic has a total of only 3 km of surface defects in addition to some areas of cloud shadow because of persistent inclement weather during data collection. The 2009 four-band image mosaic is perhaps the best image dataset that exists for the entire Arizona part of the Colorado River. Some analyses of these image mosaics do not require the full 12-bit dynamic range or all four bands of the calibrated image database, in which atmospheric scattering (or haze) had not been removed from the four bands. To provide scientists and the general public with image products that are more useful for visual interpretation, the 12-bit image data were converted to 8-bit natural-color and color-infrared images, which also removed atmospheric scattering within each wavelength-band image. The conversion required an evaluation of the

  8. Small format digital photogrammetry for applications in the earth sciences

    Science.gov (United States)

    Rieke-Zapp, Dirk

    2010-05-01

    coordinate system is important to measure the dip angle of geologic features in outcrop. The planimetric coordinate systems would be arbitrary, but may easily be oriented to compass north introducing a direction measurement of a compass. Wooden spheres and a Leica disto D3 laser distance meter added less than 0.150 kg to the field equipment considering that a suitable digital camera was already part of it. Identification of a small digital camera suitable for precise measurements was a major part of this work. A group of cameras were calibrated several times over different periods of time on a testfield. Further evaluation involved an accuracy assessment in the field comparing distances between signalized points calculated form a photogrammetric setup with coordinates derived from a total station survey. The smallest camera in the test required calibration on the job as the interior orientation changed significantly between testfield calibration and use in the field. We attribute this to the fact that the lens was retracted then the camera was switched off. Fairly stable camera geometry in a compact size camera with lens retracting system was accomplished for Sigma DP1 and DP2 cameras. While the pixel count of the cameras was less than for the Ricoh, the pixel pitch in the Sigma cameras was much larger. Hence, the same mechanical movement would have less per pixel effect for the Sigma cameras than for the Ricoh camera. A large pixel pitch may therefore compensate for some camera instability explaining why cameras with large sensors and larger pixel pitch typically yield better accuracy in object space. Both Sigma cameras weigh approximately 0.250 kg and may even be suitable for use with ultralight aerial vehicles (UAV) which have payload restriction of 0.200 to 0.300 kg. A set of other cameras that were available were also tested on a calibration field and on location showing once again that it is difficult to reason geometric stability from camera specifications. Image

  9. Creation Greenhouse Environment Map Using Localization of Edge of Cultivation Platforms Based on Stereo Vision

    Directory of Open Access Journals (Sweden)

    A Nasiri

    2017-10-01

    based on the visual odometry, global map of the environment is constructed. To evaluate the accuracy of the obtained algorithm in estimation of the position of the corners, Euclidian distances of coordinates of the corners achieved by Leica Total Station and coordinates and resulted from local maps, were computed. Results and Discussion Results showed that the lower edges have been detected with better accuracy than the upper ones. Upper edges were not desirably extracted because of being close to the pots. In contrast, due to the distance between lower edge and the ground surface, lower edges were extracted with a higher quality. Since the upper and lower edges of the platform are in the same direction, the lower edges of the platform have been only used for producing an integrated map of the greenhouse environment. The total length of the edge of the cultivation platforms was 106.6 meter, that 94.79% of which, was detected by the proposed algorithm. Some regions of the edge of the platforms were not detected, since they were not located in the view angle of the stereo camera. By the proposed algorithm, 83.33% of cultivation platforms’ corners, were detected with the average error of 0.07309 meter and mean squared error of 0.0076. Non- detected corners are due the fact that they were not located in the camera view angle. The maximum and minimum errors in the localization, according to the Euclidian distance, were 0.169 and 0.0001 meters, respectively. Conclusions Stereo vision is the perception of the depth of 3D with the disparity of the two images. In navigation, stereo vision is used for localizing the obstacles of movement. Cultivation platforms are the main obstacle of movement in greenhouses. Therefore, it is possible to design an integrated map of greenhouse environment and perform automatic control by localization of the cultivation platforms. In this research, the depth discontinuity feature in the locations of the edges, was used for the localization of the

  10. The orange-brown patina of Salisbury Cathedral (West Porch) surfaces: evidence of its man-made origin.

    Science.gov (United States)

    Martín-Gil, Jesus; Martín-Gil, Francisco Javier; del Carmen Ramos-Sánchez, Maria; Martín-Ramos, Pablo

    2005-09-01

    In this paper, we attempt to elucidate the composition and origin of the orange patina on the surfaces of the West-Porch of Salisbury Cathedral by comparison to other known patinas: (i) the orange-brown patina on the marble surfaces of the Acropolis in Athens and the Arch of Titus in Rome whose analyses have shown very high amounts of phosphates, and generally amino acids from animal-skin glue or other protein binders; (ii) the phosphated patinas which also contain oxalates, found in 1996 on Catalonian calcareous sandstones and in the calcareous dolomites of the Monastery of Silos, Spain, whose origin is either the application of calcium caseinate, or egg yolk and animal glue; and (iii) the patinas with only oxalates found in some of Verona's monuments (St. Zeno) and Spanish sites as in the Monastery of Guadalupe and Cuenca cathedral, formed either by the mineralization of algal filaments or by biological reactions yielding oxalate from yolk egg (added to stone as part of preservative empirical treatments). In the winter of 2003, the West-Porch of Salisbury Cathedral received conservation works, but the old patina was not entirely removed. This fact has allowed us to collect the samples for its study. The IR spectra were registered with a Golden Gate ATR Mk II system using attenuated total reflectance Fourier transform infrared (ATR/FTIR) spectrometry. Mineral composition was determined by XRD (Philips PW 1710 spectrometer with Cu tube), whereas major and trace elements analyses were performed by XRF (Philips PW1480 PW). Microscopy examination was performed on a Leica M655 microscope. Phosphate, oxalate, calcium and sulphate contents were analysed by usual chemical methods. ATD-FTIR spectra of the Salisbury's patina exhibit peaks at 2361, 2341 and 671 cm(-1) (assigned to phosphates); 3410, 1680, 1620, 1122 and 602 cm(-1) (assigned to sulphates); and 1447/1437 and 876 cm(-1) (attributed to carbonates). The little peaks at 1620 and 798 cm(-1) could be assigned to

  11. Airborne LIDAR and high resolution satellite data for rapid 3D feature extraction

    Science.gov (United States)

    Jawak, S. D.; Panditrao, S. N.; Luis, A. J.

    2014-11-01

    This work uses the canopy height model (CHM) based workflow for individual tree crown delineation and 3D feature extraction approach (Overwatch Geospatial's proprietary algorithm) for building feature delineation from high-density light detection and ranging (LiDAR) point cloud data in an urban environment and evaluates its accuracy by using very high-resolution panchromatic (PAN) (spatial) and 8-band (multispectral) WorldView-2 (WV-2) imagery. LiDAR point cloud data over San Francisco, California, USA, recorded in June 2010, was used to detect tree and building features by classifying point elevation values. The workflow employed includes resampling of LiDAR point cloud to generate a raster surface or digital terrain model (DTM), generation of a hill-shade image and an intensity image, extraction of digital surface model, generation of bare earth digital elevation model (DEM) and extraction of tree and building features. First, the optical WV-2 data and the LiDAR intensity image were co-registered using ground control points (GCPs). The WV-2 rational polynomial coefficients model (RPC) was executed in ERDAS Leica Photogrammetry Suite (LPS) using supplementary *.RPB file. In the second stage, ortho-rectification was carried out using ERDAS LPS by incorporating well-distributed GCPs. The root mean square error (RMSE) for the WV-2 was estimated to be 0.25 m by using more than 10 well-distributed GCPs. In the second stage, we generated the bare earth DEM from LiDAR point cloud data. In most of the cases, bare earth DEM does not represent true ground elevation. Hence, the model was edited to get the most accurate DEM/ DTM possible and normalized the LiDAR point cloud data based on DTM in order to reduce the effect of undulating terrain. We normalized the vegetation point cloud values by subtracting the ground points (DEM) from the LiDAR point cloud. A normalized digital surface model (nDSM) or CHM was calculated from the LiDAR data by subtracting the DEM from the DSM

  12. Geological setting of silica in Dehnow-Abid region (Eshghabad northeast using fluid inclusions studies

    Directory of Open Access Journals (Sweden)

    Omid Yazdanpanah

    2017-02-01

    Full Text Available Introduction Dehnow-Abid area is a part of the geological map of Eshghabad with scale 1:100000 (Aghanabati, 1994 that is located about 20 kilometers northeast of Eshghabad and in the coordinates of 57° 6´ 0" to 57° 10´ 0" eastern longitude and 34° 28´ 0" to 34 21´ 0" northern latitude. The Dehnow-Abid area is located in Tabas block and east of central Iran structural zone. The small continent east central Iran (Takin, 1972 includes blocks: Loot, Tabas and Yazd that constitute Iran's eastern part (Davoudzadeh and Schmidt, 1982. In geology, we can acquire more information about temperature forming minerals and rocks, pressure, density of the fluid and the chemical composition of the ore bearing fluids by fluid inclusions studies. Properties as well as their role in our understanding of the sources and evolution of ore bearing hydrothermal fluids and genesis of mineral deposits are very important (Rodder, 1979. In this study, we tried to use both field and laboratory studies, including petrography and thermometry studies of fluid inclusions, environment formation of quartz in the specified Dehno-Abid. Materials and methods At first, in order to identify the area, the 1:100000 map of Eshghabad was used. Then, for a complete cognition of mentioned area, after a few field visits and sampling of outcrops of quartz, we prepared 16 double polishing sections from some crystalline and milky quartz. Then, 10 thin sections of sandstones of that area were prepared for identification the host rock. Microscopic examinations on fluid inclusions were done by a LEICA DMLSP polarizing light microscope. Fluid inclusion micro-thermometry studies were done by using a Linkam THM S600 heating and freezing stage and with a TMS94 controller. Also, a cooling LNP which is mounted on an Olympus BX-41 microscope in Laboratory Fluid inclusion of Earth Sciences, Damghan University was used. Discussion and results Lithology of the Dehnow-Abid area included dark shale

  13. Virtual Microscope Views of the Apollo 11 and 12 Lunar Samples

    Science.gov (United States)

    Gibson, E. K.; Tindle, A. G.; Kelley, S. P.; Pillinger, J. M.

    2016-01-01

    components, and publication on a website. Two large research quality microscopes are used to collect all the images required for a virtual microscope. The first is part of an integrated package that utilizes Leica PowerMosaic software and a motorised XYZ stage to generate large area mosaics. It includes a fast acquisition camera and depending on the PTS size normally is used to produce seamless mosaic images consisting of 100-500 individual photographs. If the sample is suitable, three mosaics of each sample are recorded - plane polarised light, between crossed polars and reflected light. In order for the VM to be a true petrological microscope it is necessary to recreate the features of a rotating stage and perform observations using filters to produce polarised light. Thus the petrological VM includes the capability of seeing changes in optical properties (pleochroism and birefringence) during rotation allowing mineral identification. The second microscope in the system provides the functions of the rotating stage. To this microscope we have added a robotically controlled motor to acquire seventy-two images (5 degree intervals) in plane polarised light and between crossed polars. To process the images acquired from the two microscopes involves a combination of proprietary software (Photoshop) and our own in-house code. The final stage involves assembling all the components in an HTML5 environment. Pathfinder investigations: We have undertaken a number of pilot studies to demonstrate the efficacy of the petrological microscope with lunar samples. The first was to make available on-line images collected from the Educational Package of Apollo samples provided by NASA to the UK STFC (Science and Technical Facilities Council) for loan as educational material e.g. for schools. The real PTSs of the samples are now no longer sent out to schools removing the risks associated with transport, accidental breakage and eliminating the possibility of loss. The availability of lunar

  14. Premature aging phenotype in mice lacking high affinity nicotinic receptors: region specific changes in layer V pyramidal cell morphology

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    Eleni Konsolaki

    2014-02-01

    (4-6 months and old (22-24 months C57Bl6J wildtype and β2-/- mice were used in this study (WT adult n=5; WT old n=5; β2-/- adult n=5; β2-/- old n=6. Each brain was fixed overnight in 4% paraformaldehyde and subsequently transferred to saline solution until ready for use. The two hemispheres were separated and embedded in 3% agarose. Sections (100µm were cut using a vibratome from V1 (both sagittal and coronal sections and ACC (coronal sections. Complete neurons were defined as YFP+ pyramidal cells whose soma, basal dendrites, apical dendrite with its branches and apical tuft could be clearly distinguished under the fluorescence microscope. Argon laser (488 nm was used for YFP excitation, and Z-series of images were captured by Leica Confocal Software (LAS AF. Measurements were restricted to parameters that could be fully visualized and quantified within the section thickness in order to avoid errors associated with distal dendrites that would be absent due to the cutting procedure, thereby biasing our sample in favour of smaller dendritic trees. Hence, measurements were taken for the following 12 parameters, with respect to the three neuronal compartments: (i soma: volume (in 3 dimensions, for optimal estimation of absolute size; horizontal and vertical Feret diameter which gives additional information on the shape of the cell body; (ii apical dendrite: length of dendrite from soma to the apical tuft bifurcation, number of apical branches, diameter (measured at a distance of 60µm from the soma, vertical and horizontal coverage of apical tuft; (iii basal dendrites: number of primary dendrites, diameter of all primary dendrites (measurements taken at 2 µm from the soma, length of all primary dendrites (up to first bifurcation point, number of all secondary dendrites. More distal dendritic segments could not be measured reliably in all cells and were excluded from analysis. Several additional parameters were calculated from these measurements: “tuft area” was