Antioxidant and antibacterial effects of laurus nobilis aqueous extract again Staphylococcus aureus and Escherichia coli

Directory of Open Access Journals (Sweden)

B. Azimzadeh

2017-05-01

Full Text Available Some medical plants which are rich in phenolic compounds (flavnoieds, tannis and anthocyanin have drawn increasing attention as the most important naturanal antioxidant source by many developed countries. Laurus nobilis is one of the medical plants that grows in various regions of Iran. This plants is known to have many benefits and medical properties such as diuretics and mosaics. Also, this plant is used in treatment of gastrointestinal problems, especialy and it is effectieve in elimination of stomach gas. In this experiment, we studied antioxidant and antibacterial effects of Laurus nobilis plant. To this purpose, the efficiency of aqueous solvent extract, phenolic compounds, DPPH radical scavenging and ferric-reducing power and ABTS free radical scavenging were examined. Antibacterial characteristics of aqueous extract was evaluated on Staphylococcus aureus and Escherichiacoli. The result shows that the aqueous extract of Lauros nobilis has 14/8% extraction efficiency with high rate of phenolic (99/9±9/95 and the lowest IC50 in DPPH test (2/813 and the high rate of ferric reducing power (22/15±2/10 and ABTS free radical scavenging (22/87±2/03. The result of antibacterial test also indicates that aqueous extract has high antibacterial effect on Staphylococcus aureus (18±0 and E. Coli (18±0 mm.These facts show high antioxidant and antibacterial activity of laurel’s extract.

Drying characteristics of bay laurel (Laurus nobilis L.) fruits in a ...

African Journals Online (AJOL)

Jane

2011-06-24

Jun 24, 2011 ... cosmetic industry, food and medicinal purposes. The ... Fresh bay laurel fruits procured from Black Sea Region of Turkey ..... rice. ASAE Paper No: 3531, ASAE, St. Joseph, MI. Afifi FU, Khalil E, Tamimi SO, Disi A (1997).

Bionomy of the laurel scale Aonidia lauri (Bouche (Hemiptera: Diaspididae in Podgorica, Montenegro

Directory of Open Access Journals (Sweden)

Graora Draga

2016-01-01

Full Text Available Aonidia lauri (Bouche (Hemiptera: Diaspididae is a serious pest of laurel (Laurus nobilis L. in urban parts of the City of Podgorica (Montenegro. Severe infestation causes chlorotic spots and necrotic rings around feeding spots, drying and dieback of leaves and buds. In addition, plants become physiologically weak and lose in aesthetic quality, while continuous infestation in urban areas often leads to partial or complete drying of plants. This study of the biology of A. lauri on L. nobilis was carried out at three locations in Podgorica ('Stara Varoš', 'Centar' and 'Preko Morače', Montenegro, in 2010 and 2011. A. lauri developed three generations annually and overwintered on laurel leaves and branches as the second-instar nymph-larval stage. An extended period of larval development ensures a continuous presence of all development stages on plants, which leads to overlapping of generations. Sporadic predatory ladybirds, Chilocorus bipustulatus (L. (Coleoptera: Coccinellidae, whose larvae and adults feed on scales, were detected inside A. lauri colonies.

Isolation and characterization of 20 microsatellite loci for laurel species (Laurus, Lauraceae).

Science.gov (United States)

Arroyo, Juan M; Rigueiro, Cristina; Rodríguez, Rocío; Hampe, Arndt; Valido, Alfredo; Rodríguez-Sánchez, Francisco; Jordano, Pedro

2010-05-01

Microsatellite primers were developed for the evergreen tree Laurus to investigate population genetic structure and patterns of gene flow via animal-dispersed pollen and seeds. Twenty polymorphic nuclear microsatellite markers were developed using CA, GA, AAC, and ATG n-enriched genomic libraries. Given the tetraploidy of the sampled populations, we analyzed our data both as dominant loci and as codominant genotypic data to calculate allele frequencies and genetic diversity. A total of 196 and 222 alleles were found in 37 Mediterranean (L. nobilis) and 26 Macaronesian islands (L. azorica) individuals, respectively. Levels of polymorphism of the reported markers are adequate for studies of diversity and parentage in natural populations of this Tertiary relict tree.

Laurus nobilis, Zingiber officinale and Anethum graveolens essential oils: Composition, antioxidant and antibacterial activities against bacteria isolated from fish and shellfish

OpenAIRE

Snuossi, M.; Trabelsi, N.; Taleb, S. B.; Dehmeni, A.; Flamini, G.; De Feo, V.

2016-01-01

Several bacterial strains were isolated from wild and reared fish and shellfish. The identification of these strains showed the dominance of the Aeromonas hydrophila species in all seafood samples, followed by Staphylococcus spp., Vibrio alginolyticus, Enterobacter cloacae, Klebsiella ornithinolytica, Klebsiella oxytoca and Serratia odorifera. The isolates were studied for their ability to produce exoenzymes and biofilms. The chemical composition of the essential oils from Laurus nobilis leav...

Laurus nobilis, Vitex agnus-castus ve Tamarix parviflora Türlerinin Kimyasal İçeriği ve Fenolik Ekstraktiflerinin İncelenmesi

Directory of Open Access Journals (Sweden)

Samim YAŞAR

2016-05-01

Full Text Available Bu çalışmada Laurus nobilis, Vitex agnus-castus ve Tamarix parviflora bitkilerinden alınan örneklerde kimyasal bileşim ve fenolik ekstraktifler incelenmiştir. Bu türlerin endüstriyel anlamda oduna alternatif olabilirliği ve antioksidan üretiminde hammadde olarak kullanılabilirliği değerlendirilmiştir. Laurus nobilis, Vitex agnus-castus ve Tamarix parviflora bitkilerine ait örneklerde holoselüloz, selüloz, α-selüloz, lignin ve kül miktarları ile etanol siklohekzan, soğuk su, sıcak su ve %1 NaOH çözünürlükleri sırasıyla %73.26-76.32, %53.58-54.52, %43.28-44.73, %20.39-21.27, %2.12-2.79, %2.49-2.71, %9.67-11.70, %10.77-12.42 ve %21.77-24.48 aralığında bulunmuştur. HPLC analizleri toplam fenolik madde miktarının Vitex agnus-castus’da (2.09 mg/g, Tamarix parviflora’da (1.05 mg/g ve Laurus nobilis’de (0.58 mg/g olduğunu göstermiştir. Laurus nobilis’te en yüksek miktardaki fenolik ekstraktif madde epikateşin (0.23 mg/g ve Tamarix parviflora’da gallik asit (0.17 mg/g olarak belirlenmiş, Vitex agnus-castus’da ise p-hidroksibenzoik asit (0.95 mg/g olarak tespit edilmiştir. Elde edilen sonuçlar, çalışma konusu bitkilerin iğne yapraklı ve yapraklı türlerle karşılaştırılabilir düzeyde olduğunu göstermiş olup, söz konusu maki türlerinin orman ürünleri endüstrisinde ve ticari antioksidan üretiminde hammadde olarak kullanılabileceklerini ortaya koymuştur.

Antimicrobial effects of Turkish propolis, pollen, and laurel on spoilage and pathogenic food-related microorganisms.

Science.gov (United States)

Erkmen, Osman; Ozcan, Mehmet Musa

2008-09-01

The antimicrobial activities of propolis extract, pollen extract, and essential oil of laurel (Laurus nobilis L.) at concentrations from 0.02% to 2.5% (vol/vol) were investigated on bacteria (Bacillus cereus, Bacillus subtilis, Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Yersinia enterocolitica, Enterococcus faecalis, and Listeria monocytogenes), yeasts (Saccharomyces cerevisiae and Candida rugosa), and molds (Aspergillus niger and Rhizopus oryzae). Pollen has no antimicrobial effects on the bacteria and fungi tested in the concentrations used. Propolis showed a bactericidal effect at 0.02% on B. cereus and B. subtilis, at 1.0% on S. aureus and E. faecalis, and at 0.2% on L. monocytogenes. The minimum inhibitory concentration of propolis for fungi was 2.5%. Propolis and laurel were ineffective against E. coli and S. typhimurium at the concentrations tested. The results showed that the antimicrobial activity were concentration dependent. Propolis and essential oil of laurel may be used as biopreservative agents in food processing and preservation.

Evaluation of the Volatile Oil Composition and Antiproliferative Activity of Laurus nobilis L. (Lauraceae on Breast Cancer Cell Line Models

Directory of Open Access Journals (Sweden)

Rana Abu-Dahab

2014-03-01

Full Text Available Volatile oil composition and antiproliferative activity of Laurus nobilis L. (Lauraceae fruits and leaves grown in Jordan were investigated. GC-MS analysis of the essential oil of the fruits resulted in the identification of 45 components representing 99.7 % of the total oil content, while the leaf essential oil yielded 37 compounds representing 93.7% of the total oil content. Oxygenated monoterpene 1,8-cineole was the main component in the fruit and leaf oils. Using sulphorhodamine B assay; the crude ethanol fraction, among other solvent extracts, showed strong antiproliferative activity for both leaves and fruits, nevertheless, the fruits were more potent against both breast cancer cell models (MCF7 and T47D. At IC 50 values ; the mechanism of apoptosis was nevertheless different: where L. nobilis fruit proapoptotic efficacy was not regulated by either p53 or p21, L. nobilis leaf extract components enhanced the p53 levels substantially. In both extracts, apoptosis was not caspase-8 or Fas Ligand and sFas (Fas/APO-1 dependent. Our studies highlight L. nobilis as a potential natural agent for breast cancer therapy. Compared with non induced basal cells, both L. nobilis fruits and leaves induced a significant enrichment in the cytoplasmic mono- and oligonucleosomes after assumed induction of programmed MCF7 cell death.

Diversity hotspots of the laurel forest on Tenerife, Canary Islands: a phylogeographic study of Laurus and Ixanthus.

Science.gov (United States)

Betzin, Anja; Thiv, Mike; Koch, Marcus A

2016-09-01

Macaronesian laurel forest is among the worldwide hotspots of threatened biodiversity. With increasing evidence that woodland composition on the Canary Islands changed dramatically during the last few thousand years, the aim of this study was to find evidence for substantial recent population dynamics of two representative species from laurel forest. Amplified fragment length polymorphism (AFLP) was used to evaluate fine-scaled genetic variation of the paradigmatic tree Laurus novocanariensis (Lauraceae) and a long-lived herbaceous gentian from core laurel forest, Ixanthus viscosus (Gentianaceae), on Tenerife. Bioclimatic variables were analysed to study the respective climate niches. A chloroplast DNA screening was performed to evaluate additional genetic variation. Genetic diversity of the laurel tree showed severe geographic partitioning. On Tenerife, fine-scaled Bayesian clustering of genetic variation revealed a western and an eastern gene pool, separated by a zone of high admixture and with a third major gene pool. Compared with genetic clusters found on the other Canary Islands, the East-West differentiation on Tenerife seems to be more recent than differentiation between islands. This is substantiated by the finding of extremly low levels of chloroplast DNA-based polymorphisms. Ixanthus showed no geographic structuring of genetic variation. Genetic data from Tenerife indicate contemporary gene flow and dispersal on a micro/local scale rather than reflecting an old and relic woodland history. In particular for Laurus, it is shown that this species occupies a broad bioclimatic niche. This is not correlated with its respective distribution of genetic variation, therefore indicating its large potential for contemporary rapid and effective colonization. Ixanthus is more specialized to humid conditions and is mostly found in the natural Monteverde húmedo vegetation types, but even for this species indications for long-term persistence in the respective

Chemical composition and antioxidant properties of Laurus nobilis L. and Myrtus communis L. essential oils from Morocco and evaluation of their antimicrobial activity acting alone or in combined processes for food preservation.

Science.gov (United States)

Cherrat, Lamia; Espina, Laura; Bakkali, Mohammed; García-Gonzalo, Diego; Pagán, Rafael; Laglaoui, Amin

2014-04-01

This study describes the antioxidant and antimicrobial activity of Laurus nobilis L. and Myrtus communis L. essential oils (EOs). This is the first report of the synergistic antimicrobial effect of these EOs in combination with physical food preservation treatments. EOs obtained by steam distillation from aerial parts of Laurus nobilis and Myrtus communis were analysed by using gas chromatography-mass spectrometry. The main compounds were 1,8-cineole and 2-carene (L. nobilis EO); and myrtenyl acetate, 1,8-cineole and α-pinene (M. communis EO). L. nobilis EO showed higher antioxidant activity than M. communis EO in three complementary antioxidant tests. Although antimicrobial activity tests demonstrated the effectiveness of L. nobilis EO and the lack of bactericidal effect of M. communis EO, synergistic lethal effects were observed when combining each EO (0.2 µL mL(-1)) with mild heat (54°C for 10 min) or high hydrostatic pressure (175-400 MPa for 20 min). In contrast, combination of EOs with pulsed electric fields (30 kV cm(-1) for 25 pulses) showed no additional effects. This study shows the great potential of these EOs in combined treatments with mild heat and high hydrostatic pressure to obtain a higher inactivation of foodborne pathogens, which might help in the design of safe processes applied at low intensity. © 2013 Society of Chemical Industry.

In vitro antimicrobial properties of plant essential oils thymus vulgaris, cymbopogon citratus and laurus nobilis against five important foodborne pathogens Propriedades antibacterianas in vitro de óleos essenciais de thymus vulgaris, cymbopogon citratus e laurus nobilis contra cinco importantes bactérias patogênicas veiculadas por alimentos

Directory of Open Access Journals (Sweden)

Alessandra Farias Millezi

2012-03-01

Full Text Available Several essential oils of condiment and medicinal plants possess proven antimicrobial activity and are of important interest for the food industry. Therefore, the Minimum Inhibitory Concentrations (MIC of those oils should be determined for various bacteria. MIC varies according to the oil used, the major compounds, and the physiology of the bacterium under study. In the present study, the essential oils of the plants Thymus vulgaris (time, Cymbopogon citratus (lemongrass and Laurus nobilis (bay were chemically quantified, and the MIC was determined on the bacteria Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19117, Salmonella enterica Enteritidis S64, and Pseudomonas aeruginosa ATCC 27853. The essential oil of C. citratus demonstrated bacterial activity at all concentrations tested and against all of the bacteria tested. The majority of essential oil compounds were geranial and neral. The major constituent of T. vulgaris was 1.8-cineol and of L. nobilis was linalool, which presented lower antibacterial activity, followed by 1.8-cineol. The Gram-negative bacteria demonstrated higher resistance to the use of the essential oils tested in this study. E. coli was the least sensitive and was inhibited only by the oils of C. citratus and L. nobilis.Diversos óleos essenciais de plantas condimentares e medicinais possuem atividade antimicrobiana comprovada, sendo de grande interesse para a indústria de alimentos. Dessa forma, as Concentrações Mínimas Inibitórias (CMI desses óleos para diversas bactérias devem ser determinadas. As CMI variam de acordo com o óleo utilizado, dos compostos majoritários e da fisiologia da bactéria em estudo. Na presente pesquisa, os óleos essenciais das plantas Thymus vulgaris (tomilho, Cymbopogon citratus (capim-limão e Laurus nobilis (louro foram quantificados quimicamente e determinou-se a CMI sobre as bactérias Staphylococcus aureus ATCC 25923, Escherichia. coli

Differences in the fragrances of pollen and different floral parts of male and female flowers of Laurus nobilis.

Science.gov (United States)

Flamini, Guido; Cioni, Pier Luigi; Morelli, Ivano

2002-07-31

The headspace analyses of pollen, whole living female and male flowers, and staminoids have been performed on Laurus nobilis L. (Lauraceae) from Italy to determine whether there are differences in the volatiles emitted in order to give a contribution to the roles of the different flower parts in the pollination ecology of dioecious plants. Also, the essential oils obtained from male and female plants have been studied to evaluate a possible correlation between the spontaneously emitted volatiles and the constituents stored in the glandular tissues. Furthermore, the headspace sampling technique has been improved, with respect to previously employed methods, by means of solid-phase microextraction (SPME).

21 CFR 582.20 - Essential oils, oleoresins (solvent-free), and natural extractives (including distillates).

Science.gov (United States)

2010-04-01

.... Clover Trifolium spp. Coca (decocainized) Erythroxylum coca Lam. and other spp. of Erythroxylum. Coffee Coffea spp. Cola nut Cola acuminata Schott and Endl., and other spp. of Cola. Coriander Coriandrum... Cola acuminata Schott and Endl., and other spp. of Cola. Laurel berries Laurus nobilis L. Laurel leaves...

Nutritional and antioxidant contributions of Laurus nobilis L. leaves: would be more suitable a wild or a cultivated sample?

Science.gov (United States)

Dias, Maria Inês; Barros, Lillian; Dueñas, Montserrat; Alves, Rita C; Oliveira, M Beatriz P P; Santos-Buelga, Celestino; Ferreira, Isabel C F R

2014-08-01

Medicinal and aromatic plants are used since ancient times in folk medicine and traditional food, but also in novel pharmaceutical preparations. The controversy lies in the use of cultivated and/or wild plants presenting both advantages and disadvantages in biological, ecological but also economic terms. Herein, cultivated and wild samples of Laurus nobilis L. were chemically characterized regarding nutritional value, free sugars, organic acids, fatty acids and tocopherols. Furthermore, the antioxidant activity (scavenging activity, reducing power and lipid peroxidation inhibition) and individual phenolic profile of L. nobilis extracts and infusions were evaluated. Data showed that the wild sample gave higher nutritional contribution related to a higher content of proteins, free sugars, organic acids, PUFA and tocopherols. It also gave better PUFA/SFA and n-6/n-3 ratios. Regarding antioxidant activity and phenolic compounds, it was the cultivated sample (mostly the infusion) that showed the highest values. The present study supports the arguments defending the use of wild and cultivated medicinal and aromatic plants as both present very interesting features, whether nutritional or antioxidant, that can be an assessed by their consumption. In vitro culture could be applied to L. nobilis as a production methodology that allows combination of the benefits of wild and cultivated samples. Copyright © 2014 Elsevier Ltd. All rights reserved.

In vitro antimicrobial properties of plant essential oils thymus vulgaris, cymbopogon citratus and laurus nobilis against five important foodborne pathogens

Directory of Open Access Journals (Sweden)

Alessandra Farias Millezi

2012-03-01

Full Text Available Several essential oils of condiment and medicinal plants possess proven antimicrobial activity and are of important interest for the food industry. Therefore, the Minimum Inhibitory Concentrations (MIC of those oils should be determined for various bacteria. MIC varies according to the oil used, the major compounds, and the physiology of the bacterium under study. In the present study, the essential oils of the plants Thymus vulgaris (time, Cymbopogon citratus (lemongrass and Laurus nobilis (bay were chemically quantified, and the MIC was determined on the bacteria Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19117, Salmonella enterica Enteritidis S64, and Pseudomonas aeruginosa ATCC 27853. The essential oil of C. citratus demonstrated bacterial activity at all concentrations tested and against all of the bacteria tested. The majority of essential oil compounds were geranial and neral. The major constituent of T. vulgaris was 1.8-cineol and of L. nobilis was linalool, which presented lower antibacterial activity, followed by 1.8-cineol. The Gram-negative bacteria demonstrated higher resistance to the use of the essential oils tested in this study. E. coli was the least sensitive and was inhibited only by the oils of C. citratus and L. nobilis.

Erythema multiforme like allergic contact dermatitis associated with laurel oil: a rare presentation.

Science.gov (United States)

Uzuncakmak, Tugba Kevser; Karadag, Ayse Serap; Izol, Belcin; Akdeniz, Necmettin; Cobanoglu, Bengu; Taskin, Secil

2015-04-16

Allergic contact dermatitis is a common skin disease, which affects approximately 20% of the population. This reaction may present with several clinical manifestations. Erythema multiforme-like allergic contact dermatitis is a rare type of non-eczematous contact dermatitis, which may lead to difficulty in diagnosis.Essential oil of Laurus nobilis is widely used in massage therapy for antiinflammatory and analgesic effects. Laurus nobilis induced contact dermatitis has been reported in the literature but an erythema multiforme-like presentation is rare.

Optimization of the spray application technology in bay laurel (Laurus nobilis).

Science.gov (United States)

Nuyttens, D; Braekman, P; Foque, D

2009-01-01

Bay laurel is an evergreen, commercially grown and expensive ornamental pot plant, which is susceptible to different pests like aphids, scale and lerp insects, thrips, caterpillars of codling moth and sooty moulds. Recently, caterpillars of the Mediterranean carnation leafroller (Cacoecimorpha pronubana) cause more and more problems. These pests can lead to important financial losses for the growers. During summer the individual pot plants are placed on a field-container in a fairly dense configuration. Crop protection is traditionally done by moving with a spray lance between the rows of pot plants and treating each individual plant from bottom to top. Good penetration is clearly an important advantages of this spray technique but it is very time-consuming, unhealthy and laborious. Some other growers use a 'spray platform' on a high-clearance tractor. Plants sprayed from this platform are exclusively approached from above resulting in an inferior spray deposition on the lower parts of the plants. To overcome the disadvantages of both available techniques, the potential of an automated tunnel sprayer was investigated. Five different nozzle types were evaluated under laboratory conditions i.e. hollow cone, standard flat fan, air inclusion flat fan, deflector flat fan and twin air inclusion flat fan at spray pressures varying from 3.0 to 7.0 bar depending on the type of nozzle. For each nozzle type, three nozzle sizes were included in the experiments which resulted in 15 different spray application techniques. All experiments were done at a speed of 2.5 km x h(-1). This resulted in three different application volumes: 2450, 4900 and 7300 l x ha(-1). After optimizing the nozzle configuration (distance and orientation) using water-sensitive paper, deposition tests with five different mineral chelates as tracer elements were performed. Filter papers were used as collectors at 20 different positions to measure spray deposition, distribution and penetration in the canopy

Experimental study of water desorption isotherms and thin-layer convective drying kinetics of bay laurel leaves

Science.gov (United States)

Ghnimi, Thouraya; Hassini, Lamine; Bagane, Mohamed

2016-12-01

The aim of this work is to determine the desorption isotherms and the drying kinetics of bay laurel leaves ( Laurus Nobilis L.). The desorption isotherms were performed at three temperature levels: 50, 60 and 70 °C and at water activity ranging from 0.057 to 0.88 using the statistic gravimetric method. Five sorption models were used to fit desorption experimental isotherm data. It was found that Kuhn model offers the best fitting of experimental moisture isotherms in the mentioned investigated ranges of temperature and water activity. The Net isosteric heat of water desorption was evaluated using The Clausius-Clapeyron equation and was then best correlated to equilibrium moisture content by the empirical Tsami's equation. Thin layer convective drying curves of bay laurel leaves were obtained for temperatures of 45, 50, 60 and 70 °C, relative humidity of 5, 15, 30 and 45 % and air velocities of 1, 1.5 and 2 m/s. A non linear regression procedure of Levenberg-Marquardt was used to fit drying curves with five semi empirical mathematical models available in the literature, The R2 and χ2 were used to evaluate the goodness of fit of models to data. Based on the experimental drying curves the drying characteristic curve (DCC) has been established and fitted with a third degree polynomial function. It was found that the Midilli Kucuk model was the best semi-empirical model describing thin layer drying kinetics of bay laurel leaves. The bay laurel leaves effective moisture diffusivity and activation energy were also identified.

In Vitro Study of the Antifungal Activity of Essential Oils Obtained from Mentha spicata, Thymus vulgaris, and Laurus nobilis.

Science.gov (United States)

Houicher, Abderrahmane; Hechachna, Hind; Teldji, Hanifa; Ozogul, Fatih

2016-01-01

The aim of this study was to determine the antifungal activity of the essential oils isolated from three aromatic plants against 13 filamentous fungal strains. The major constituents of Mentha spicata, Thymus vulgaris, and Laurus nobilis essential oils were carvone (52.2%), linalool (78.1%), and 1,8-cineole (45.6%), respectively. There are also some patents suggesting the use of essential oils as natural and safe alternatives to fungicides for plant protection. In the present work, M. spicata essential oil exhibited the strongest activity against all tested fungi in which Fusarium graminearum, F.moniliforme, and Penicillium expansum were the most sensitive to mint oil with lower minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) values of 2.5 μL mL-1 (v/v). Thymus vulgaris essential oil was less active compared to the oil of M. spicata. Aspergillus ochraceus was the most sensitive strain to thyme oil with MIC and MFC values of 2.5 and 5 μL mL-1, respectively. Thymus vulgaris essential oil also exhibited a moderate fungicidal effect against the tested fungi, except for A. niger (MFC >20 μL-1). L. nobilis essential oil showed a similar antifungal activity with thyme oil in which A. parasiticus was the most resistant strain to this oil (MFC >20 μL mL-1). Our findings suggested the use of these essential oils as alternatives to synthetic fungicides in order to prevent pre-and post-harvest infections and ensure product safety. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Activité antioxydante et anti-candidosique de l’huile essentielle de Laurus nobilis L. provenant de la région d’El Kala (Nord–Est Algérien

Directory of Open Access Journals (Sweden)

Amira OUIBRAHIM

2015-12-01

Full Text Available L’huile essentielle extraite de Laurus nobilis L. par hydrodistillation a fourni un rendement de 0,7%. L’analyse de l’huile essentielle par CG/SM a permis l’identification de 17 composants, principalement des monoterpènes. 1,8 cinéole (36,31%, β-linalol (22.52%, eugenol-methylether (9.17% et camphene (7.37% ont constitué les composés majoritaires avec un total de 96,95%. L’activité antioxydante in vitro a été évaluée à travers trois méthodes: le test de piégeage du radical libre DPPH avec un IC50 de 1,55 ± 0,14 mg/ml ; le test de blanchiment de β-carotène qui a permis d’enregistrer un pourcentage d’inhibition de la peroxydation lipidique de 41,97% et finalement l’évaluation du pouvoir réducteur qui s’est avéré faible par rapport à celui de l’acide ascorbique. L’activité anticantidosique a été testée sur 4 souches de Candida albicans en utilisant la méthode de dilution en milieu gélosé. Les quatre souches ont montré une sensibilité avec une CMI de 0,68 mg/ml pour les souches d’origine urinaire et vaginale et 1.08 mg/ml pour les Candida d’origine bucco-nasale. L’huile essentielle de Laurus nobilis originaire de la région d’El Kala a manifesté des propriétés antioxidantes modérées et des propriétés anticandidosiques.

Supercritical CO2 extract and essential oil of bay (Laurus nobilis L. – chemical composition and antibacterial activity

Directory of Open Access Journals (Sweden)

JASNA IVANOVIĆ

2010-03-01

Full Text Available The present study deals with the supercritical carbon dioxide (SC-CO2 extraction and hydrodistillation (HD of dried bay leaves (Laurus nobilis L.. The chemical composition and antibacterial activity of the SC-CO2 extract and essential oil (EO from dried leaves of bay were compared to each other and literature data. Qualitative and quantitative analyses of the SC-CO2 extract and EO were performed using GC–FID and GC–MS analytical methods. A significant difference in the chemical composition of the SC-CO2 extract and EO was observed. The EO comprised high contents of monoterpenes and their oxygenated derivates (98.4 %, principally 1,8-cineole (33.4 %, linalool (16.0 % and α-terpinyl acetate (13.8 %, sabinene (6.91 % and methyl eugenol (5.32 %. The SC-CO2 extract comprised twice less monoterpenes and their oxygenated derivates (43.89 %, together with sesquiterpenes (12.43 %, diterpenes (1.33 % and esters (31.13 %. The major components were methyl linoleate (16.18 %, α-terpinyl acetate (12.88 %, linalool (9.00 %, methyl eugenol (8.67 %, methyl arachidonate (6.28 % and eugenol (6.14 %. An investigation of the antibacterial activity of bay SC-CO2 extract and EO was completed on different Staphylococcus strains using the broth macrodilution method. Staphylococcus intermedius strains were the most susceptible to both the SC-CO2 extract and EO (MIC = 640 µg/ml.

Etude de l’activité antibactérienne des huiles essentielles d’Inula viscosa, Salvia officinalis et Laurus nobilis de la région de Bejaia

Directory of Open Access Journals (Sweden)

Nawel Kheyar

2014-02-01

Full Text Available Dans cette étude, l’activité antibactérienne des huiles essentielles d’Inula viscosa, Salvia officinalis et Laurus nobilis a été évaluée in vitro par la méthode de diffusion sur gélose contre des souches de référence et multirésistante : il s’agit de Staphylococcus aureus (ATCC 6538, Pseudomonas aeruginosa (ATCC 27853, Escherichia coli (NAR, Klebsiella pneumoniae (E47 et Listeria innocua (CLIP 74915. Pseudomonas aeruginosa a été l’espèce la plus résistante, cependant, Staphylococcus aureus s’est montrée la plus sensible aux huiles testées. Les résultats de l’analyse chimique de la composition de l’huile Inula viscosa par chromatographie en phase gazeuse indiquent la richesse de cette dernière en thymol (6,93% and carvacrol (2,27%, ce qui pourrait être à l’origine des résultats observés.

Extraction and Separation of Volatile and Fixed Oils from Berries of Laurus nobilis L. by Supercritical CO2

Directory of Open Access Journals (Sweden)

M. Assunta Dessì

2008-08-01

Full Text Available Isolation of volatile and fixed oils from dried berries of Laurus nobilis L. from Tunisia have been obtained by supercritical fractioned extraction with carbon dioxide. Extraction experiments were carried out at a temperature of 40 °C and pressures of 90 and 250 bar. The extraction step performed at 90 bar produced a volatile fraction mainly composed of (E-β-ocimene (20.9%, 1,8-cineole (8.8%, α-pinene (8.0%, β-longipinene (7.1%, linalool acetate (4.5%, cadinene (4.7%, β-pinene (4.2%, α-terpinyl acetate (3.8% and α-bulnesene (3.5%. The oil yield in this step of the process was 0.9 % by weight charged. The last extraction step at 250 bar produced an odorless liquid fraction, in which a very small percentage of fragrance compounds was found, whereas triacylglycerols were dominant. The yield of this step was 15.0 % by weight. The most represented fatty acids of the whole berry fixed oil were 12:0 (27.6%, 18:1 n-9 (27.1%, 18:2 n-6 (21.4%, and 16:0 (17,1%, with the 18:1 n-9 and 18:2 n-6 unsaturated fatty acids in particular averaging 329 μg/mg of oil.

Does short-term potassium fertilization improve recovery from drought stress in laurel?

Science.gov (United States)

Oddo, Elisabetta; Inzerillo, Simone; Grisafi, Francesca; Sajeva, Maurizio; Salleo, Sebastiano; Nardini, Andrea

2014-08-01

Xylem hydraulic conductance varies in response to changes in sap solute content, and in particular of potassium (K(+)) ion concentration. This phenomenon, known as the 'ionic effect', is enhanced in embolized stems, where it can compensate for cavitation-induced loss of hydraulic conductance. Previous studies have shown that in well-watered laurel plants (Laurus nobilis L.), potassium concentration of the xylem sap and plant hydraulic conductance increased 24 h after fertilization with KCl. The aim of this work was to test whether water-stressed laurel plants, grown under low potassium availability, could recover earlier from stress when irrigated with a KCl solution instead of potassium-free water. Two-year-old potted laurel seedlings were subjected to water stress by suspending irrigation until leaf conductance to water vapour (g(L)) dropped to ∼30% of its initial value and leaf water potential (ψ(L)) reached the turgor loss point (ψ(TLP)). Plants were then irrigated either with water or with 25 mM KCl and monitored for water status, gas exchange and plant hydraulics recovery at 3, 6 and 24 h after irrigation. No significant differences were found between the two experimental groups in terms of ψ(L), g(L), plant transpiration, plant hydraulic conductance or leaf-specific shoot hydraulic conductivity. Analysis of xylem sap potassium concentration showed that there were no significant differences between treatments, and potassium levels were similar to those of potassium-starved but well-watered plants. In conclusion, potassium uptake from the soil solution and/or potassium release to the xylem appeared to be impaired in water-stressed plants, at least up to 24 h after relief from water stress, so that fertilization after the onset of stress did not result in any short-term advantage for recovery from drought. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Distillation Parameters for Pilot Plant Production of Laurus nobilis Essential oil

Directory of Open Access Journals (Sweden)

Temel Özek

2012-01-01

Full Text Available Essential oils have increasing importance in flavour and fragrance industries. They are obtained by distillation techniques. In order to produce an oil with market potential its optimum production parameters have to be well known prior to its commercial production. Determination of the steam distillation parameters of commercially available Laurel leaves oil in pilot plant scale is described. The effect of steam rate and processing time play a major role in distillation of essential oils. Distillation speed was high in the beginning of the process, then gradually reduced as the distillation proceeded. The main component of the oil of Laurel leaf oil was 1,8-cineole accumulating significantly in the early fractions.

Laurel leaf extracts for honeybee pest and disease management: antimicrobial, microsporicidal, and acaricidal activity.

Science.gov (United States)

Damiani, Natalia; Fernández, Natalia J; Porrini, Martín P; Gende, Liesel B; Álvarez, Estefanía; Buffa, Franco; Brasesco, Constanza; Maggi, Matías D; Marcangeli, Jorge A; Eguaras, Martín J

2014-02-01

A diverse set of parasites and pathogens affects productivity and survival of Apis mellifera honeybees. In beekeeping, traditional control by antibiotics and molecules of synthesis has caused problems with contamination and resistant pathogens. In this research, different Laurus nobilis extracts are tested against the main honeybee pests through an integrated point of view. In vivo effects on bee survival are also evaluated. The ethanol extract showed minimal inhibitory concentration (MIC) values of 208 to 416 μg/mL, having the best antimicrobial effect on Paenibacillus larvae among all substances tested. Similarly, this leaf extract showed a significant antiparasitic activity on Varroa destructor, killing 50 % of mites 24 h after a 30-s exposure, and on Nosema ceranae, inhibiting the spore development in the midgut of adult bees ingesting 1 × 10(4) μg/mL of extract solution. Both ethanol extract and volatile extracts (essential oil, hydrolate, and its main component) did not cause lethal effects on adult honeybees. Thus, the absence of topical and oral toxicity of the ethanol extract on bees and the strong antimicrobial, microsporicidal, and miticidal effects registered in this study place this laurel extract as a promising integrated treatment of bee diseases and stimulates the search for other bioactive phytochemicals from plants.

Efecto de la poda sobre la cantidad y el rendimiento del aceite esencial de Laurus nobilis

Directory of Open Access Journals (Sweden)

Ana María Callejas-Leguizamon

2013-07-01

Full Text Available Objetivo. Conocer el comportamiento de la planta de laurel con diferentes podas, en cuanto a su rendimiento y cantidad de aceite esencial para satisfacer las demandas del mercado. Materiales y métodos. Se monitorearon 48 plantas, ubicadas en el municipio de Nuevo Colón, Boyacá, en un diseño completamente al azar, con tres tratamientos (deshoje, despunte y el testigo sin poda en 5 estadios fenológicos, con cuatro repeticiones. Se evaluó la respuesta en cuanto a tres solventes usados en la extracción y diferentes tipos de poda. Resultados. El hexano fue el solvente que permitió mayor extracción de aceite esencial de las hojas de laurel, seguido de etanol y cloroformo-metanol, respectivamente. Asimismo, en los diferentes tratamientos de poda se demostró que la mayor cantidad de aceite esencial (p≤0,01 se obtuvo en hojas de laurel con despunte, mientras que con deshoje se obtuvo la menor cantidad. Analizando el rendimiento, se encontraron rendimientos (2,32% en las hojas de laurel con despunte, seguido de las plantas testigo (2,05% y por último las plantas con deshoje (1,24%. Conclusiones. El despunte, como práctica agronómica, en laurel representa mayores beneficios al productor y es una alternativa de valor agregado.

EVALUATION DE L'ACTIVITE ANTIBACTERIENNE

African Journals Online (AJOL)

kamal

Activité antibactérienne de l'huile essentielle des inflorescences de Laurus. Nobilis et Lavandula Angustifolia ... Les huiles essentielles des fleurs de Lavandula officinalis et les feuilles de Laurus nobilis inhibent la croissance de Staphylococus aureus .... protocole adopté est le suivant : • incorporation de la fraction de ...

Ecophysiological and biochemical strategies of response to ozone in Mediterranean evergreen broadleaf species

Science.gov (United States)

Nali, C.; Paoletti, E.; Marabottini, R.; Della Rocca, G.; Lorenzini, G.; Paolacci, A. R.; Ciaffi, M.; Badiani, M.

Three Mediterranean shrubs, Phillyrea latifolia L. (phillyrea), Arbutus unedo L. (strawberry tree), and Laurus nobilis L. (laurel), differing in their morphological and ecological response to water shortage, were exposed for 90 days to 0 or 110 ppb of ozone (O 3), 5 h each day. This yielded an accumulated exposure over of a threshold of 40 ppb (AOT40) of 31.5 ppm h over the 3 months experiment. These species showed differing responses to O 3: laurel and phillyrea developed foliar chlorotic mottles on the adaxial surface of leaves, whereas strawberry tree leaves showed reddish interveinal stipple-like necrotic lesions. In all cases, however, foliar injury did not exceed 8% of the sampled leaf area. At the end of the exposure period, O 3-induced stomatal limitation caused significant decreases of net photosynthesis in strawberry tree and laurel, but not in phillyrea. The relative water content of the leaves was significantly decreased by O 3, especially in laurel and strawberry tree, suggesting the occurrence of drought stress. Electrical conductivity of leachates from foliar discs increased in response to the treatment, much more strongly in laurel and in strawberry tree than in phillyrea, suggesting an O 3-dependent alteration of the membrane retention capacity. At the end of the experimental period, the activity of superoxide dismutase and the content of reduced glutathione, but not that of reduced ascorbate, were significantly increased in the ozonated leaves of strawberry tree and phillyrea, but not in laurel. The evergreen broadleaves studied here maybe relatively tolerant to realistic O 3 levels, at least in terms of visible injury and gas exchange. Such tolerance might overlap with their level of tolerance to drought stress. High constitutive levels, and/or O 3-induced increases in antioxidants, might contribute to O 3 tolerance in these Mediterranean evergreen broadleaf species.

Maceration of Extra Virgin Olive Oil with Common Aromatic Plants Using Ultrasound-Assisted Extraction: An UV-Vis Spectroscopic Investigation

Directory of Open Access Journals (Sweden)

Ozren Jović

2018-01-01

Full Text Available Rosemary (Rosmarinus officinalis, garden sage (Salvia officinalis, summer savory (Satureja hortensis, laurel (Laurus nobilis, and other aromatic plants were put in olive oil and exposed to ultrasounds for different duration. Filtrated oils were dissolved in cyclohexane, and UV-Vis measurements were carried out. Absorbance values corresponding to chlorophylls, carotenoids, flavonoids (370 nm, and polyphenols (around 300 nm were measured. In addition, for some samples, total phenols were determined using Folin-Denis reagent and compared with the similar maceration procedure in water solvent (instead of olive oil. Maceration without ultrasound in olive oil for each plant was also compared with ultrasound-assisted extraction. The results show that significant amount of aromatic content can be extracted in olive oil by applying ultrasounds for only few minutes, especially for Salvia officinalis powder. The use of UV-Vis measurements is simple but enough to examine the extent of phenol content extraction through such maceration procedure.

Antibacterial and Antioxidant Activities of Essential Oils from Artemisia herba-alba Asso., Pelargonium capitatum × radens and Laurus nobilis L.

Science.gov (United States)

Rafiq, Ragina; Hayek, Saeed A.; Anyanwu, Ugochukwu; Hardy, Bonita I.; Giddings, Valerie L.; Ibrahim, Salam A.; Tahergorabi, Reza; Kang, Hye Won

2016-01-01

Essential oils are natural antimicrobials that have the potential to provide a safer alternative to synthetic antimicrobials currently used in the food industry. Therefore, the aim of this study was to evaluate the antimicrobial and antioxidant activities of essential oils from white wormwood, rose-scented geranium and bay laurel against Salmonella typhimurium and Escherichia coli O157:H7 on fresh produce and to examine consumer acceptability of fresh produce treated with these essential oils. Our results showed that essential oil derived from rose-scented geranium exhibited the most effective antimicrobial activity at the same and similar minimum inhibition concentration levels (0.4%, v/v and 0.4% and 0.5%, v/v) respectively against Salmonella typhimurium and Escherichia coli O157:H7. All three essential oils showed antioxidant properties, with the highest activity occurring in bay laurel essential oil. In a sensory test, tomatoes, cantaloupe and spinach sprayed with 0.4% rose-scented geranium essential oil received higher scores by panelists. In conclusion, rose-scented geranium essential oil could be developed into a natural antimicrobial to prevent contamination of Salmonella typhimurium and Escherichia coli O157:H7 in fresh produce, plus this oil would provide additional health benefits due to the antioxidant properties of its residue. PMID:28231123

Identification, Functional Characterization, and Evolution of Terpene Synthases from a Basal Dicot1[OPEN

Science.gov (United States)

Yahyaa, Mosaab; Matsuba, Yuki; Brandt, Wolfgang; Doron-Faigenboim, Adi; Bar, Einat; McClain, Alan; Davidovich-Rikanati, Rachel; Lewinsohn, Efraim; Pichersky, Eran; Ibdah, Mwafaq

2015-01-01

Bay laurel (Laurus nobilis) is an agriculturally and economically important dioecious tree in the basal dicot family Lauraceae used in food and drugs and in the cosmetics industry. Bay leaves, with their abundant monoterpenes and sesquiterpenes, are used to impart flavor and aroma to food, and have also drawn attention in recent years because of their potential pharmaceutical applications. To identify terpene synthases (TPSs) involved in the production of these volatile terpenes, we performed RNA sequencing to profile the transcriptome of L. nobilis leaves. Bioinformatic analysis led to the identification of eight TPS complementary DNAs. We characterized the enzymes encoded by three of these complementary DNAs: a monoterpene synthase that belongs to the TPS-b clade catalyzes the formation of mostly 1,8-cineole; a sesquiterpene synthase belonging to the TPS-a clade catalyzes the formation of mainly cadinenes; and a diterpene synthase of the TPS-e/f clade catalyzes the formation of geranyllinalool. Comparison of the sequences of these three TPSs indicated that the TPS-a and TPS-b clades of the TPS gene family evolved early in the evolution of the angiosperm lineage, and that geranyllinalool synthase activity is the likely ancestral function in angiosperms of genes belonging to an ancient TPS-e/f subclade that diverged from the kaurene synthase gene lineages before the split of angiosperms and gymnosperms. PMID:26157114

Laurus nobilis Linn, Murraya koenigii

African Journals Online (AJOL)

1Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State,. Qwaqwa Campus, Phuthaditjhaba ... the Central Spices Market in Mile 12 area, Ketu,. Lagos, Nigeria. The plants were ... magnesium oxide (14 mM) and 20 µL test sample (0.25 – 1.0 mg/mL extract).

Evaluación de sistemas de captación de agua en restauración forestal de suelos degradados

Directory of Open Access Journals (Sweden)

Gloria María Cifuentes Molano

2016-06-01

Full Text Available El objetivo del estudio consistió en caracterizar las variables explicativas en la captación de humedad, evaluadas a través   de unidades sistematizadas o microcuencas de tipo negarim y semicircular. Igualmente se tomaron en cuenta las variables temperatura, precipitación y cambios en el crecimiento,  como la altura de la planta, el diámetro del tallo y el fuste en la especie sembrada Laurus nobilis L.  Mediante análisis ANOVA se comparó la humedad contenida en la zona de recepción, en donde se plantó el árbol; este análisis es una extensión de la prueba T para dos muestras independientes en  el caso de diseños con más de dos muestras, en donde la variable independiente es el tipo de tratamiento utilizado (Microcuenca negarim y semicircular. Se estimó la viabilidad de la especie Laurel (Laurus nobilis L de la familia de las lauráceas, arbusto con el cual se reforestó el área objeto de estudio. Se evalúo el nivel de escorrentía en cada uno de los sistemas de captación y los efectos hidrológicos para el almacenamiento de humedad, utilizando para el tratamiento   los datos meteorológicos de la zona de estudio y aplicando el Modelo Hridrológico Modipé. El contenido de humedad de las parcelas varió, así,  el 66% de las microcuencas semicirculares tuvieron una humedad en el suelo mayor a 29%; en tanto que en las microcuencas negarim solo el 23% de las unidades presentaron un porcentaje de humedad  entre el   23%  y el 29%. Los resultados de este trabajo contribuyen a orientar procesos y prácticas de recolección de aguas lluvias para revegetalización de suelos áridos y erosionados en zonas de baja precipitación.

Laurels for Laurel Oaks

Science.gov (United States)

Sisson, Ray

1976-01-01

Describes a former Air Force base converted into a joint school district vocational school which includes among other things an up-to-date facility for the students, located at Laurel Oaks, one of the four campuses of the Great Oaks Joint Vocational School District. (HF)

In vitro screening of selected essential oils from medicinal plants ...

African Journals Online (AJOL)

Enteric methane production lowers the efficiency of feed utilization in ruminants and contributes to greenhouse gas emissions which are responsible for global climate change. This study examined the effects of nine essential oils (EO) from Citrus aurantifolia, Cymbopogon citratus, Eucalyptus citriodora, Laurus nobilis, ...

Microsatellite loci isolated from the scleractinian coral, Acropora nobilis.

Science.gov (United States)

Isomura, Naoko; Hidaka, Michio

2008-05-01

We report the isolation and characterization of eight microsatellite loci from the scleractinian coral, Acropora nobilis. The microsatellite loci were obtained using compound SSR primers or an enrichment protocol. All the loci were polymorphic with four to eight alleles per locus and observed heterozygosities ranging from 0.22 to 0.76. Some of the primers developed for the two congeners, Acropora palmata and Acropora millepora were applicable to A. nobilis. These loci are useful for studying the connectivity among A. nobilis populations in Okinawa, southern Japan. © 2007 The Authors.

Fattening performance, blood parameters and slaughter traits of ...

African Journals Online (AJOL)

Jane

2011-07-11

Jul 11, 2011 ... traits of Karya lambs consuming blend of essential oil compounds ... the antioxidative properties of plant extracts, which can ... of thyme leaf (Origanum onites L.), daphne leaf (Laurus nobilis L.), sage tea leaf (Salvia triloba L.), fennel seed (Foeniculum vulgare L.), orange ..... Antimicrobial Feed Additives.

Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent Phytophthora infestans.

Science.gov (United States)

Soylu, E Mine; Soylu, Soner; Kurt, Sener

2006-02-01

The aim of this study was to find an alternative to synthetic fungicides currently used in the control of devastating oomycete pathogen Phytophthora infestans, causal agent of late blight disease of tomato. Antifungal activities of essential oils obtained from aerial parts of aromatic plants such as oregano (Origanum syriacum var. bevanii), thyme (Thymbra spicata subsp. spicata), lavender (Lavandula stoechas subsp. stoechas), rosemary (Rosmarinus officinalis), fennel (Foeniculum vulgare), and laurel (Laurus nobilis), were investigated against P. infestans. Both contact and volatile phase effects of different concentrations of the essential oils used were determined by using two in vitro methods. Chemical compositions of the essential oils were also determined by GC-MS analysis. Major compounds found in essential oils of thyme, oregano, rosemary, lavender, fennel and laurel were carvacrol (37.9%), carvacrol (79.8), borneol (20.4%), camphor (20.2%), anethole (82.8%) and 1,8-cineole (35.5%), respectively. All essential oils were found to inhibit the growth of P. infestans in a dose-dependent manner. Volatile phase effect of oregano and thyme oils at 0.3 microg/ml air was found to completely inhibit the growth of P. infestans. Complete growth inhibition of pathogen by essential oil of fennel, rosemary, lavender and laurel was, however, observed at 0.4-2.0 microg/ml air concentrations. For the determination of the contact phase effects of the tested essential oils, oregano, thyme and fennel oils at 6.4 microg/ml were found to inhibit the growth of P. infestans completely. Essential oils of rosemary, lavender and laurel were inhibitory at relatively higher concentrations (12.8, 25.6, 51.2 microg/ml respectively). Volatile phase effects of essential oils were consistently found to be more effective on fungal growth than contact phase effect. Sporangial production was also inhibited by the essential oil tested. Light and scanning electron microscopic (SEM) observation on

Interactive ion-mediated sap flow regulation in olive and laurel stems: physicochemical characteristics of water transport via the pit structure.

Science.gov (United States)

Ryu, Jeongeun; Ahn, Sungsook; Kim, Seung-Gon; Kim, TaeJoo; Lee, Sang Joon

2014-01-01

Sap water is distributed and utilized through xylem conduits, which are vascular networks of inert pipes important for plant survival. Interestingly, plants can actively regulate water transport using ion-mediated responses and adapt to environmental changes. However, ionic effects on active water transport in vascular plants remain unclear. In this report, the interactive ionic effects on sap transport were systematically investigated for the first time by visualizing the uptake process of ionic solutions of different ion compositions (K+/Ca2+) using synchrotron X-ray and neutron imaging techniques. Ionic solutions with lower K+/Ca2+ ratios induced an increased sap flow rate in stems of Olea europaea L. and Laurus nobilis L. The different ascent rates of ionic solutions depending on K+/Ca2+ ratios at a fixed total concentration increases our understanding of ion-responsiveness in plants from a physicochemical standpoint. Based on these results, effective structural changes in the pit membrane were observed using varying ionic ratios of K+/Ca2+. The formation of electrostatically induced hydrodynamic layers and the ion-responsiveness of hydrogel structures based on Hofmeister series increase our understanding of the mechanism of ion-mediated sap flow control in plants.

Empleo de aceites esenciales como antioxidantes naturales

Directory of Open Access Journals (Sweden)

Zydgalo, J. A.

1995-10-01

Full Text Available The aims of this study were to determine and compare the antioxidant effect of essential oils from Thymus vulgaris, Laurus nobilis, Foeniculum vulgare, Eucalyptus globules, Tagetes minuta, Satureja parvifolia and Lippia polystachya in soybean oil. The concentrations of volatile oils in soybean oil were 0.1% and 0.02%. The antioxidant activity was measured by peroxide values. Thymus vulgaris and L. nobilis essential oils exhibited a significant antioxidant activity, followed by F. vulgare and E. globules in a decreasing order. Tagetes minuta, S. parvifolia and L. polystachya had not effect, and their contribution to the stability of soybean oil was negligible.

Los objetivos de este estudio fueron determinar y comparar el efecto antioxidante de los aceites esenciales de Thymus vulgaris, Laurus nobilis, Foeniculum vulgare, Eucalyptus globulus, Tagetes minuta, Satureja parvifolia y Lippia polystachya en el aceite de soja. Las concentraciones de las esencias en el aceite de soja fueron 0.1% y 0.02%. La actividad antioxidante fue medida por los valores de peróxido. Los aceites esenciales de T. vulgaris y L nobilis exhibieron una significativa acción antioxidante seguidos, en orden decreciente, por F. vulgare y E. globulus. Las esencias de T. minuta, S. parvifolia y L polystachya no mostraron efecto antioxidante y su contribución a la estabilidad oxidativa del aceite de soja fue despreciable.

Novel Paraconiothyrium species on stone fruit trees and other woody hosts.

Science.gov (United States)

Damm, U; Verkley, G J M; Crous, P W; Fourie, P H; Haegi, A; Riccioni, L

2008-06-01

Coniothyrium-like fungi are common wood and soil inhabitants and hyperparasites on other fungi. They belong to different fungal genera within the Pleosporales. Several isolates were obtained on wood of different Prunus species (plum, peach and nectarine) from South Africa, on Actinidia species from Italy and on Laurus nobilis from Turkey. Morphological and cultural characteristics as well as DNA sequence data (5.8S nrDNA, ITS1, ITS2, partial SSU nrDNA) were used to characterise them. The isolates belonged to three species of the recently established genus Paraconiothyrium. This is the first report of Paraconiothyrium brasiliense on Prunus spp. from South Africa. Two new species are described, namely Paraconiothyrium variabile sp. nov. on Prunus persica and Prunus salicina from South Africa, on Actinidia spp. from Italy and on Laurus nobilis from Turkey, and Paraconiothyrium africanum sp. nov. on Prunus persica from South Africa. Although other known species of Paraconiothyrium commonly produce aseptate conidia, those of P. africanum and P. hawaiiense comb. nov. are predominantly two-celled.

Complete Chloroplast Genome of the Wollemi Pine (Wollemia nobilis): Structure and Evolution.

Science.gov (United States)

Yap, Jia-Yee S; Rohner, Thore; Greenfield, Abigail; Van Der Merwe, Marlien; McPherson, Hannah; Glenn, Wendy; Kornfeld, Geoff; Marendy, Elessa; Pan, Annie Y H; Wilton, Alan; Wilkins, Marc R; Rossetto, Maurizio; Delaney, Sven K

2015-01-01

The Wollemi pine (Wollemia nobilis) is a rare Southern conifer with striking morphological similarity to fossil pines. A small population of W. nobilis was discovered in 1994 in a remote canyon system in the Wollemi National Park (near Sydney, Australia). This population contains fewer than 100 individuals and is critically endangered. Previous genetic studies of the Wollemi pine have investigated its evolutionary relationship with other pines in the family Araucariaceae, and have suggested that the Wollemi pine genome contains little or no variation. However, these studies were performed prior to the widespread use of genome sequencing, and their conclusions were based on a limited fraction of the Wollemi pine genome. In this study, we address this problem by determining the entire sequence of the W. nobilis chloroplast genome. A detailed analysis of the structure of the genome is presented, and the evolution of the genome is inferred by comparison with the chloroplast sequences of other members of the Araucariaceae and the related family Podocarpaceae. Pairwise alignments of whole genome sequences, and the presence of unique pseudogenes, gene duplications and insertions in W. nobilis and Araucariaceae, indicate that the W. nobilis chloroplast genome is most similar to that of its sister taxon Agathis. However, the W. nobilis genome contains an unusually high number of repetitive sequences, and these could be used in future studies to investigate and conserve any remnant genetic diversity in the Wollemi pine.

Mountain laurel toxicosis in a dog.

Science.gov (United States)

Manhart, Ingrid O; DeClementi, Camille; Guenther, Christine L

2013-01-01

To describe a case of mountain laurel (Kalmia latifolia) toxicosis in a dog, including case management and successful outcome. A dog presented for vomiting, hematochezia, bradycardia, weakness, and ataxia, which did not improve with supportive treatment. Mountain laurel ingestion was identified as cause of clinical signs after gastrotomy was performed to remove stomach contents. Supportive treatment was continued and the dog made a full recovery. This report details a case of mountain laurel toxicosis in a dog, including management strategies and outcome, which has not been previously published in the veterinary literature. © Veterinary Emergency and Critical Care Society 2013.

Erythema multiforme due to contact with laurel oil.

Science.gov (United States)

Athanasiadis, G I; Pfab, F; Klein, A; Braun-Falco, M; Ring, J; Ollert, M

2007-08-01

Erythema multiforme is a relatively common skin disorder. The best known cause is herpes simplex virus infection. We report the first case of erythema multiforme due to contact with laurel oil. The diagnosis was confirmed by the positive patch test to laurel oil, the histopathological studies of the lesions and the histopathological studies of the positive patch test to laurel oil.

78 FR 67084 - Drawbridge Operation Regulation; Broad Creek, Laurel, DE

Science.gov (United States)

2013-11-08

...-AA09 Drawbridge Operation Regulation; Broad Creek, Laurel, DE AGENCY: Coast Guard, DHS. ACTION: Notice....25, both at Laurel, DE. The proposed new rule would change the current regulation by requiring a..., mile 8.2, all at Laurel, shall open on signal if at least 48 hours notice is given. Previous regulation...

Chemical composition and antimicrobial activity of essential oils from selected herbs cultivated in the South of Brazil against food spoilage and foodborne pathogens Composição química e atividade antimicrobiana de óleos essenciais de plantas selecionadas cultivadas no Sul do Brasil contra micro-organismos patogênicos e deteriorantes de alimentos

Directory of Open Access Journals (Sweden)

Sheila Mello da Silveira

2012-07-01

Full Text Available The chemical composition of 10 selected plant essential oils obtained by steam distillation was determined by GC and GC/MS. The antimicrobial activity of the essential oils was screened against 12 important food-related bacterial strains by agar disc-diffusion assay. MIC and MBC were determined for the essential oils that presented the highest activity in the agar disc-diffusion test. The most active essential oils against the tested bacteria were, in descending order, lemongrass (Cymbopogon flexuosus, basil (Ocimum basilicum, oregano (Origanum vulgare, cinnamon leaf (Cinnamomum zeylanicum, and laurel (Laurus nobilis. Except for S. Typhimurium, the tested bateria were inhibited at MIC values lower or equal to 0.62mg mL-1 by lemongrass (Cymbopogon flexuosus essential oil. Yersinia enterocolitica presented the highest sensitivity to all essential oils tested (CMI≤0.62mg mL-1. There was a significant correlation (PA composição química de 10 óleos essenciais obtidos por destilação a vapor foi determinada por CG/DIC e CG/EM. A atividade antimicrobiana dos óleos essenciais foi detectada através do método de difusão em ágar frente a 12 espécies de bactérias de importância em alimentos. As CMI e CMB foram determinadas para os óleos essenciais que na difusão em ágar evidenciaram maior atividade. Os óleos essenciais que apresentaram maior atividade contra as bactérias testadas foram, em ordem decrescente, os de capim-limão (Cymbopogon flexuosus, manjericão (Ocimum basilicum, orégano (Origanum vulgare, folha de canela (Cinnamomum zeylanicum e louro (Laurus nobilis. Com exceção de S. Typhimurium, o óleo essencial de capim limão (Cymbopogon flexuosus apresentou valores de CMI e CMB iguais ou inferiores a 0,62mg mL-1 contra os micro-organismos testados. Yersinia enterocolitica foi o patógeno mais sensível frente a todos os óleos essenciais avaliados (CMI≤0,62mg mL-1. Foi detectada correlação significativa (P<0,05 entre os n

Ambrosia beetles associated with laurel wilt of avocado

Science.gov (United States)

Redbay ambrosia beetle, Xyleborus glabratus, is an exotic wood-boring pest first detected in 2002 near Savannah, Georgia. The beetle’s dominant fungal symbiont, Raffaelea lauricola, is the pathogen that causes laurel wilt, a lethal disease of trees in the family Lauraceae. Laurel wilt has since spr...

Laurel Springs & DoDEA

National Research Council Canada - National Science Library

Jhung, Seung

2000-01-01

At the request of the client organization, Laurel Springs School, we developed an in-depth market analysis of comparable educational programs offered within the Department of Defense Education Activities (DoDEA...

Forædling af nordmannsgran og nobilis:

DEFF Research Database (Denmark)

Nielsen, Ulrik Braüner

Denne rapport er udarbejdet som en status efter godt 7 års forædlingsarbejde i Skov & Landskab regi indenfor træarterne nobilis og nordmannsgran. Rapporten belyser status over det hidtige forædlingsarbejde og skitserer tillige nogle fremtidige perspektiver ved en fortsat forædlingsindsats...

Microbial growth and quorum sensing antagonist activities of herbal plants extracts.

Science.gov (United States)

Al-Hussaini, Reema; Mahasneh, Adel M

2009-09-03

Antimicrobial and antiquorum sensing (AQS) activities of fourteen ethanolic extracts of different parts of eight plants were screened against four Gram-positive, five Gram-negative bacteria and four fungi. Depending on the plant part extract used and the test microorganism, variable activities were recorded at 3 mg per disc. Among the Grampositive bacteria tested, for example, activities of Laurus nobilis bark extract ranged between a 9.5 mm inhibition zone against Bacillus subtilis up to a 25 mm one against methicillin resistant Staphylococcus aureus. Staphylococcus aureus and Aspergillus fumigatus were the most susceptible among bacteria and fungi tested towards other plant parts. Of interest is the tangible antifungal activity of a Tecoma capensis flower extract, which is reported for the first time. However, minimum inhibitory concentrations (MIC's) for both bacteria and fungi were relatively high (0.5-3.0 mg). As for antiquorum sensing activity against Chromobacterium violaceum, superior activity (>17 mm QS inhibition) was associated with Sonchus oleraceus and Laurus nobilis extracts and weak to good activity (8-17 mm) was recorded for other plants. In conclusion, results indicate the potential of these plant extracts in treating microbial infections through cell growth inhibition or quorum sensing antagonism, which is reported for the first time, thus validating their medicinal use.

Microbial Growth and Quorum Sensing Antagonist Activities of Herbal Plants Extracts

Directory of Open Access Journals (Sweden)

Reema Al-Hussaini

2009-09-01

Full Text Available Antimicrobial and antiquorum sensing (AQS activities of fourteen ethanolic extracts of different parts of eight plants were screened against four Gram-positive, five Gram-negative bacteria and four fungi. Depending on the plant part extract used and the test microorganism, variable activities were recorded at 3 mg per disc. Among the Grampositive bacteria tested, for example, activities of Laurus nobilis bark extract ranged between a 9.5 mm inhibition zone against Bacillus subtilis up to a 25 mm one against methicillin resistant Staphylococcus aureus. Staphylococcus aureus and Aspergillus fumigatus were the most susceptible among bacteria and fungi tested towards other plant parts. Of interest is the tangible antifungal activity of a Tecoma capensis flower extract, which is reported for the first time. However, minimum inhibitory concentrations (MIC's for both bacteria and fungi were relatively high (0.5-3.0 mg. As for antiquorum sensing activity against Chromobacterium violaceum, superior activity (>17 mm QS inhibition was associated with Sonchus oleraceus and Laurus nobilis extracts and weak to good activity (8-17 mm was recorded for other plants. In conclusion, results indicate the potential of these plant extracts in treating microbial infections through cell growth inhibition or quorum sensing antagonism, which is reported for the first time, thus validating their medicinal use.

Case Study: North Laurel High School, London, Kentucky.

Science.gov (United States)

Southern Regional Education Board, Atlanta, GA.

When North Laurel High School, London, Kentucky, opened in Fall 1992, students and teachers entered a new facility and a new era of commitment to excellence for all students. In Spring 1993, North Laurel joined the Southern Regional Education Board's High Schools That Work initiative. The new school replaced the general track and raised graduation…

Prescribed Burning For Laurel and Rhodendron Control in the Southern appalachians

Science.gov (United States)

Ralph M. Hooper

1969-01-01

Prescribed fire shows promise as a tool for the control of laurel and rhododendron in the Southern Appalachian mountains. A recent prescribed fire killed the tops of 70 percent of ail laurel under 0.5 inch d.b.h. and 70 percent of the rhododendron under 1 inch d.b.h. Seventeen months after the bum, almost all of the top-killed laurel and rhododendron have...

Effects of Herbal Essential Oil Mixture as a Dietary Supplement on Egg Production in Quail

Directory of Open Access Journals (Sweden)

Metin Çabuk

2014-01-01

Full Text Available One hundred and eighty 7-week-old laying quail were fed various diets over a 12-week period. The diets included a control diet (without essential oil mixture (EOM or antibiotics (ANTs, a basal diet including EOM (24 mg/kg feed, and a basal diet including an ANT (avilamycin, 10 mg/kg feed. Each treatment comprised 4 replications with 4 cages (15 quail per cage, amounting to 60 quail per treatment group. Diets (in mash form and water were provided for ad libitum consumption. EOM consisted of 6 different essential oils derived from the following herbs: oregano (Origanum sp., laurel leaf (Laurus nobilis L., sage leaf (Salvia triloba L., myrtle leaf (Myrtus communis, fennel seeds (Foeniculum vulgare, and citrus peel (Citrus sp.. In comparison with the control diet, adding supplements such as EOM and ANTs to the basal diet increased egg production in quail (P<0.001. However, egg production was similar between EOM and ANT treatment groups. Moreover, there were no differences between the treatment groups with regard to egg weight. Feed intake was not affected by EOM or ANT supplementation, whereas feed conversion ratio was significantly improved by EOM and ANT supplementation. Thus, we concluded that EOM has beneficial effects as a dietary supplement on egg production and feed conversion ratio.

Oviposition Deterrent and Larvicidal and Pupaecidal Activity of Seven Essential Oils and their Major Components against Culex quinquefasciatus Say (Diptera: Culicidae): Synergism–antagonism Effects

Science.gov (United States)

Andrade-Ochoa, Sergio; Sánchez-Aldana, Daniela; Chacón-Vargas, Karla Fabiola; Rivera-Chavira, Blanca E.; Camacho, Alejandro D.; Nogueda-Torres, Benjamín

2018-01-01

The larvicidal activity of essential oils cinnamon (Cinnamomum verum J. Presl), Mexican lime (Citrus aurantifolia Swingle) cumin (Cuminum cyminum Linnaeus), clove (Syzygium aromaticum (L.) Merr. & L.M.Perry), laurel (Laurus nobilis Linnaeus), Mexican oregano (Lippia berlandieri Schauer) and anise (Pimpinella anisum Linnaeus)) and their major components are tested against larvae and pupae of Culex quinquefasciatus Say. Third instar larvae and pupae are used for determination of lethality and mortality. Essential oils with more than 90% mortality after a 30-min treatment are evaluated at different time intervals. Of the essential oils tested, anise and Mexican oregano are effective against larvae, with a median lethal concentration (LC50) of 4.7 and 6.5 µg/mL, respectively. Anise essential oil and t-anethole are effective against pupae, with LC50 values of 102 and 48.7 µg/mL, respectively. Oregano essential oil and carvacrol also have relevant activities. A kinetic analysis of the larvicidal activity, the oviposition deterrent effect and assays of the effects of the binary mixtures of chemical components are undertaken. Results show that anethole has synergistic effects with other constituents. This same effect is observed for carvacrol and thymol. Limonene shows antagonistic effect with β-pinene. The high larvicidal and pupaecidal activities of essential oils and its components demonstrate that they can be potential substitutes for chemical compounds used in mosquitoes control programs. PMID:29443951

Detection of laurel wilt disease in avocado using low altitude aerial imaging.

Science.gov (United States)

de Castro, Ana I; Ehsani, Reza; Ploetz, Randy C; Crane, Jonathan H; Buchanon, Sherrie

2015-01-01

Laurel wilt is a lethal disease of plants in the Lauraceae plant family, including avocado (Persea americana). This devastating disease has spread rapidly along the southeastern seaboard of the United States and has begun to affect commercial avocado production in Florida. The main objective of this study was to evaluate the potential to discriminate laurel wilt-affected avocado trees using aerial images taken with a modified camera during helicopter surveys at low-altitude in the commercial avocado production area. The ability to distinguish laurel wilt-affected trees from other factors that produce similar external symptoms was also studied. RmodGB digital values of healthy trees and laurel wilt-affected trees, as well as fruit stress and vines covering trees were used to calculate several vegetation indices (VIs), band ratios, and VI combinations. These indices were subjected to analysis of variance (ANOVA) and an M-statistic was performed in order to quantify the separability of those classes. Significant differences in spectral values among laurel wilt affected and healthy trees were observed in all vegetation indices calculated, although the best results were achieved with Excess Red (ExR), (Red-Green) and Combination 1 (COMB1) in all locations. B/G showed a very good potential for separate the other factors with symptoms similar to laurel wilt-affected trees, such as fruit stress and vines covering trees, from laurel wilt-affected trees. These consistent results prove the usefulness of using a modified camera (RmodGB) to discriminate laurel wilt-affected avocado trees from healthy trees, as well as from other factors that cause the same symptoms and suggest performing the classification in further research. According to our results, ExR and B/G should be utilized to develop an algorithm or decision rules to classify aerial images, since they showed the highest capacity to discriminate laurel wilt-affected trees. This methodology may allow the rapid detection

Detection of laurel wilt disease in avocado using low altitude aerial imaging.

Directory of Open Access Journals (Sweden)

Ana I de Castro

Full Text Available Laurel wilt is a lethal disease of plants in the Lauraceae plant family, including avocado (Persea americana. This devastating disease has spread rapidly along the southeastern seaboard of the United States and has begun to affect commercial avocado production in Florida. The main objective of this study was to evaluate the potential to discriminate laurel wilt-affected avocado trees using aerial images taken with a modified camera during helicopter surveys at low-altitude in the commercial avocado production area. The ability to distinguish laurel wilt-affected trees from other factors that produce similar external symptoms was also studied. RmodGB digital values of healthy trees and laurel wilt-affected trees, as well as fruit stress and vines covering trees were used to calculate several vegetation indices (VIs, band ratios, and VI combinations. These indices were subjected to analysis of variance (ANOVA and an M-statistic was performed in order to quantify the separability of those classes. Significant differences in spectral values among laurel wilt affected and healthy trees were observed in all vegetation indices calculated, although the best results were achieved with Excess Red (ExR, (Red-Green and Combination 1 (COMB1 in all locations. B/G showed a very good potential for separate the other factors with symptoms similar to laurel wilt-affected trees, such as fruit stress and vines covering trees, from laurel wilt-affected trees. These consistent results prove the usefulness of using a modified camera (RmodGB to discriminate laurel wilt-affected avocado trees from healthy trees, as well as from other factors that cause the same symptoms and suggest performing the classification in further research. According to our results, ExR and B/G should be utilized to develop an algorithm or decision rules to classify aerial images, since they showed the highest capacity to discriminate laurel wilt-affected trees. This methodology may allow the

77 FR 33560 - Southwest Pennsylvania Railroad Company-Acquisition Exemption-Laurel Hill Development Corporation

Science.gov (United States)

2012-06-06

... Pennsylvania Railroad Company--Acquisition Exemption-- Laurel Hill Development Corporation Southwest... 49 CFR 1150.41 to acquire a 0.66-mile line of railroad owned by Laurel Hill Development Corporation... rail line. Most recently, in Southwest Pennsylvania Railroad Company--Acquisition Exemption--Laurel...

Eye trauma in Laurel and Hardy movies - another nice mess.

Science.gov (United States)

Zegers, Lara DA; Zegers, Richard Hc

2016-11-01

One of the characteristics in Laurel and Hardy films is a lot of physical violence. The present study examines the occurrence of eye trauma in Laurel and Hardy movies and discusses the impact they could have been had if the films were set in reality. All 92 movies starring Laurel and Hardy as a pair in leading roles were watched together by the authors and were scored for any eye trauma. Eighty-eight eye traumas happened, of which 48% were directed at Hardy. The eye poke was the most frequently occurring eye trauma and the traumatic corneal abrasion was very likely the most frequently occurring injury. Among the most serious causes of eye trauma were the pin of a door handle, a stick, a champagne cork, a tree branch and tacks. Without a doubt, if their films had been reality, especially Hardy but also Laurel and several other people, would have suffered from serious eye injuries caused by the 88 eye traumas. The findings of the present study might reflect the personality, character and intellectual capacity of both Laurel and Hardy as 'Two Minds Without a Single Thought'. © The Author(s) 2016.

The potential anticonvulsant activity of the ethanolic extracts of Achillea nobilis and Momordica charantia in rats

Directory of Open Access Journals (Sweden)

Gamal A. Soliman

2016-06-01

Full Text Available Context: Currently available antiepileptic drugs have debilitating adverse effects. Natural products and plants already used in traditional medicine can be a good place to start in the search for safer and more effective options. Aims: To investigate the anticonvulsant potential of Achillea nobilis and Momordica charantia extracts in maximal electroshock (MES, as well as pentylenetetrazole (PTZ- and strychnine nitrate (STN- induced seizure models in rats. Methods: For each model, eight groups of 21-day-old male Albino rats were used. The 1st group was kept as control, 2nd as standard (diazepam, 7.5 mg/kg; 3rd – 5th treated with A. nobilis (100, 200 and 300 mg/kg; and 6th – 8th administered M. charantia (100, 200 and 300 mg/kg. After 30 min, rats were exposed to a shock of 150 mA by a convulsiometer, via ear electrodes for 2 s (in MES test or sc injection of PTZ (85 mg/kg or STN (2.5 mg/kg. Results: A. nobilis and M. charantia extracts (200 and 300 mg/kg demonstrated dose-dependent anticonvulsant effect against MES-induced seizures. In the PTZ induced convulsion, A. nobilis and M. charantia (200 and 300 mg/kg significantly slowed the commencement of convulsions and minimized the duration of seizures. A. nobilis (300 mg/kg showed 60% protection in rats against STN induced seizures. In contrast, A. nobilis (100 and 200 mg/kg and M. charantia (100, 200 and 300 mg/kg showed no significant protection against STN-induced seizures in rats. Conclusions: The results of the present study suggest that both extracts exhibited marked anticonvulsant activities.

Mycorrhizal dependency of laurel (Ocotea sp.)

International Nuclear Information System (INIS)

Sierra-Escobar, Jorge A; Castro Restrepo, Dagoberto; Osorio Vega, Walter

2009-01-01

A greenhouse experiment was carried out to determine the mycorrhizal dependency of laurel (>Ocotea sp.). In order to do this, a completely randomized experimental design was used, with six treatments in a factorial array of 3 x 2 and five repetitions. The treatments involved a combination of three Phosphorus (P) levels in soil solution (0.002, 0.02 and 0.2 mg L-1) and two levels of mycorrhizal inoculation, either inoculated or non-inoculated with Glomus aggregatum Schenck and Smith. The leaf P content as a function of time was used as an output variable. Shoot dry matter, shoot P content, mycorrhizal colonization of roots, and mycorrhizal dependence were measured at harvest. The results indicated that the leaf P content increased significantly when using the mycorrhizal inoculation in laurel at P level 0.2 mg L -1, but not in the other P levels, on some of the sampling days. Shoot dry weight and total plant P content did not increase at all levels of soil available P. Mycorrhizal dependency of laurel reached 28%, which allows this species to be classified as moderately dependent on mycorrhiza.

Spatial distribution modelling of the endangered bivalve Pinna nobilis in a Marine Protected Area

Directory of Open Access Journals (Sweden)

M. VÁZQUEZ-LUIS

2014-09-01

Full Text Available The spatial distribution of Pinna nobilis densities have been analysed through a geostatistical approach in the MPA of Cabrera National Park, Balearic Islands (Spain, Western Mediterranean Sea. Regression kriging was used to model the effect of environmental variables on the density of living individuals of P. nobilis and generate a predictive map of its distribution within the MPA. The environmental variables considered for the model were: depth; slope; habitat type and heterogeneity; wave exposure; and MPA zoning. A total of 378 transects were randomly distributed with a total of 149,000 m2 surveyed at a depth range from 4.2 to 46 m. The recorded P. nobilis densities are among the highest in the Mediterranean Sea. With respect to the prediction model, results indicate that benthic habitats play a key role in the spatial distribution of P. nobilis, with higher densities in seagrass meadows of Posidonia oceanica. The fan mussel population density peaked at 9 m depth, decreasing with depth. Also, decreasing densities are expected with increasing exposure to waves. The predicted map shows some hotspots of density different in size and distributed along the MPA, and provides valuable information for the spatial conservation management of this species.

ANTIOXIDANT CAPACITY, MINERAL CONTENT AND ESSENTIAL OIL COMPOSITION FROM SELECT ALGERIAN MEDICINAL PLANTS

Directory of Open Access Journals (Sweden)

Hadjira Guenane1

2017-10-01

Full Text Available The objective of the present study was to analyze the total antioxidant capacity, minerals contents of four plants (Juniperus oxycedrus, Thymus capitatus, Laurus nobilis and Eruca vesicaria and chemical composition of the essential oils of the aerial parts of T. capitatus. Their antioxidant activity was assessed by DPPH, ABTS and FRAPS assays. Total phenol and flavonoid contents of the extracts were also determined. The results showed that the L. nobilis extract had the highest total phenolic and flavonoids contents (19.11 ± 0.22 mg GAE•g-1 dw, 4.47 ± 0.12 mg QE•g-1 dw, respectively. The extract of E. vesicaria had the highest value of TEAC for scavenging DPPH, whereas L. nobilis extract was active for ABTS and FRAP. GC/MS analysis revealed that the essential oil from the aerial parts of T. capitatus contained thirty-seven compounds; thymol was the major constituent (82.79 %. Atomic absorption spectroscopy showed high levels of Ca, K, Mg and Fe, and trace amounts of Zn, Cu and Mn in all four extracts.

Summer survival of Phytophthora ramorum in California bay laurel leaves

Science.gov (United States)

Elizabeth J. Fichtner; David M. Rizzo; Shannon C. Lynch; Jennifer Davidson; Gerri Buckles; Jennifer Parker

2008-01-01

Sudden oak death manifests as non-lethal foliar lesions on bay laurel (Umbellularia californica), which support sporulation and survival of Phytophthora ramorum in forest ecosystems. Infected bay laurel leaves are more likely to abscise than uninfected leaves, resulting in an accumulation of inoculum at the forest floor. The pathogen survives the dry...

Recovery plan for laurel wilt of avocado, caused by Raffaelea lauricola

Science.gov (United States)

Laurel wilt kills American members of the Lauraceae plant family, including avocado (Persea americana), an important commercial fruit crop. The disease threatens commercial production in the US and other countries, and currently impacts the avocado industry in Florida. As laurel wilt spreads, the N...

Sperm density required for inducing gynogenetic haploidy in scallop Chlamys nobilis

Digital Repository Service at National Institute of Oceanography (India)

Goswami, U.

Experiments conducted for standardizing sperm density for artificial insemination and inducing gynogenesis by ultra-violet rays irradiated sperms in Chlamys nobilis showed that 10:1 ratio of sperms to eggs, gave maximum rate of fertilization...

77 FR 2128 - Southwest Pennsylvania Railroad Company-Acquisition Exemption-Laurel Hill Development Corporation

Science.gov (United States)

2012-01-13

... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [Docket No. FD 35584] Southwest Pennsylvania Railroad Company--Acquisition Exemption-- Laurel Hill Development Corporation Southwest... 49 CFR 1150.41 to acquire a number of rail lines now owned by Laurel Hill Development Corporation...

Disjunct population of redbay ambrosia beetle and laurel wilt disease discovered in Mississippi

Science.gov (United States)

J.J. Riggins; M. Hughes; J.A. Smith; R. Chapin

2011-01-01

Laurel wilt is an aggressive, non-native vascular wilt disease of redbay trees (Persea borbonia), sassafras (Sassafras albidum), and other plants within the Lauraceae family. The laurel wilt pathogen, (Raffaelea lauricola), is vectored by the redbay ambrosia beetle (Xyleborus glabratus), which...

Utilization of muddy detritus as organic matter source by the fan mussel Pinna nobilis.

Directory of Open Access Journals (Sweden)

S. TRIGOS

2014-10-01

Full Text Available The knowledge of the feeding habits in marine species is fundamental to better understand their relationship with the environment. Although phytoplankton has been traditionally reported as the main food source consumed by the Mediterranean fan mussel Pinna nobilis, recent studies have revealed that detritus represents an important food source for this species. We analysed the degree of acceptance of muddy detritus and the utilisation of its organic matter (OM by P. nobilis on a group of 21 individuals (30.3-59.7 cm of total shell height (Ht. The specimens were collected between July and September 2012 in two areas (43°04´25” N; 5°46´7” E and 43°04´34” N; 5°47´32” E of the Embiez archipelago, northwestern Mediterranean (France. Our studies show that P. nobilis retains high quantities of OM from muddy detritus (47.50 ± 11.23% of filtered OM irrespectively of shell size. Smaller individuals, however, actively filter more detritus than large ones. The values of retained OM, together with previous studies on stomach contents, suggest that muddy detritus is a more important OM source than phytoplankton for this species.

RNA sequencing supports distinct reactive oxygen species-mediated pathways of apoptosis by high and low size mass fractions of Bay leaf (Lauris nobilis) in HT-29 cells.

Science.gov (United States)

Rodd, Annabelle L; Ververis, Katherine; Sayakkarage, Dheeshana; Khan, Abdul W; Rafehi, Haloom; Ziemann, Mark; Loveridge, Shanon J; Lazarus, Ross; Kerr, Caroline; Lockett, Trevor; El-Osta, Assam; Karagiannis, Tom C; Bennett, Louise E

2015-08-01

Anti-proliferative and pro-apoptotic effects of Bay leaf (Laurus nobilis) in mammalian cancer and HT-29 adenocarcinoma cells have been previously attributed to effects of polyphenolic and essential oil chemical species. Recently, we demonstrated differentiated growth-regulating effects of high (HFBL) versus low molecular mass (LFBL) aqueous fractions of bay leaf and now confirm by comparative effects on gene expression, that HFBL and LFBL suppress HT-29 growth by distinct mechanisms. Induction of intra-cellular lesions including DNA strand breakage by extra-cellular HFBL, invoked the hypothesis that iron-mediated reactive oxygen species with capacity to penetrate cell membrane, were responsible for HFBL-mediated effects, supported by equivalent effects of HFBL in combination with γ radiation. Activities of HFBL and LFBL were interpreted to reflect differentiated responses to iron-mediated reactive oxygen species (ROS), occurring either outside or inside cells. In the presence of LFBL, apoptotic death was relatively delayed compared with HFBL. ROS production by LFBL mediated p53-dependent apoptosis and recovery was suppressed by promoting G1/S phase arrest and failure of cellular tight junctions. In comparison, intra-cellular anti-oxidant protection exerted by LFBL was absent for extra-cellular HFBL (likely polysaccharide-rich), which potentiated more rapid apoptosis by producing DNA double strand breaks. Differentiated effects on expression of genes regulating ROS defense and chromatic condensation by LFBL versus HFBL, were observed. The results support ferrous iron in cell culture systems and potentially in vivo, can invoke different extra-cellular versus intra-cellular ROS-mediated chemistries, that may be regulated by exogenous, including dietary species.

Ação antioxidante de chás e condimentos de grande consumo no Brasil

Directory of Open Access Journals (Sweden)

Selene M. de Morais

Full Text Available Antioxidantes são compostos que atuam inibindo e/ou diminuindo os efeitos desencadeados pelos radicais livres e compostos oxidantes. Os chás são bebidas populares e fontes significativas de compostos fenólicos, sendo considerados importantes integrantes das dietas devido às suas propriedades antioxidantes. O presente trabalho teve como objetivo avaliar a ação antioxidante de uma variedade de chás e condimentos mais consumidos no Brasil. Os chás analisados foram das plantas: Pneumus boldus Mold., Matricaria recutita L., Cymbopogon citratus (DC Stapf, Baccharis trimera (Less. DC, Camelia sinensis (L O. Kuntze (fermentado, Camelia sinensis (não-fermentado, Lippia alba N.E. Brown,Mentha arvensis L., e Pyrus malus L. Os condimentos analisados foram: Eugenia aromatica Baill, Cinnamonum zeylanicum Blume, Laurus nobilis L. e Origanum vulgare L. O método utilizado para avaliar a ação antioxidante foi o da atividade seqüestradora de radicais livres DPPH em solução metanólica. Todas as amostras analisadas demonstraram atividade em suas diferentes concentrações. Camelia sinensis (não-fermentada foi a mais ativa com CI50= 0,14 mg/mL, cujos principais compostos antioxidantes são epigalocatequinas. Os condimentos mais ativos foram Cinnamonum zeylanicum (CI50 = 0,37 mg/mL, Eugenia aromatica Baill (CI50 = 0,46 mg/mL e Laurus nobilis (CI50 = 0,76 mg/mL, cujo principal antioxidante relatado foi o eugenol.

Recovery Plan for Laurel Wilt of Avocado

Science.gov (United States)

Laurel wilt kills American members of the Lauraceae plant family, including avocado (Persea americana). The disease threatens commercial avocado production in Florida, as well as the National Germplasm Repository for avocado in Miami (USDA-ARS). Elsewhere in the US, major (California) and minor comm...

Human Stressors Are Driving Coastal Benthic Long-Lived Sessile Fan Mussel Pinna nobilis Population Structure More than Environmental Stressors.

Science.gov (United States)

Deudero, Salud; Vázquez-Luis, Maite; Álvarez, Elvira

2015-01-01

Coastal degradation and habitat disruption are severely compromising sessile marine species. The fan shell Pinna nobilis is an endemic, vulnerable species and the largest bivalve in the Mediterranean basin. In spite of species legal protection, fan shell populations are declining. Models analyzed the contributions of environmental (mean depth, wave height, maximum wave height, period of waves with high energy and mean direction of wave source) versus human-derived stressors (anchoring, protection status, sewage effluents, fishing activity and diving) as explanatory variables depicting Pinna nobilis populations at a mesoscale level. Human stressors were explaining most of the variability in density spatial distribution of fan shell, significantly disturbing benthic communities. Habitat protection affected P. nobilis structure and physical aggression by anchoring reveals a high impact on densities. Environmental variables instead played a secondary role, indicating that global change processes are not so relevant in coastal benthic communities as human-derived impacts.

Progression and Impact of Laurel Wilt Disease within Redbay and Sassafras Populations in Southeast Georgia

Science.gov (United States)

R. Scott Cameron; James Hanula; Stephen Fraedrich; Chip Bates

2015-01-01

Laurel wilt disease (LWD), caused by the fungus Raffaelea lauricola and transmitted by Xyleborus glabratus (Redbay Ambrosia Beetle [RAB]), has killed millions of Persea borbonia (Redbay) trees throughout the southeastern Coastal Plain. Laurel wilt also has been...

Effects of environmental variables on the survival of Phytophthora ramorum in bay laurel leaves

Science.gov (United States)

M.V. DiLeo; R.M. Bostock; D.M. Rizzo

2008-01-01

Bay laurel (Umbellularia californica (Hook. & Arn.) Nutt.) is the primary reservoir host of Phytophthora ramorum Werres, De Cock & Man n?t Veld in coastal California woodlands. Non-lethal foliar lesions on bay laurel trees support the majority of pathogen sporulation during the winter et season and appear to provide the...

Turmeric and laurel aqueous extracts exhibit in vitro anti-atherosclerotic activity and in vivo hypolipidemic effects in a zebrafish model.

Science.gov (United States)

Jin, Seori; Hong, Joo-Heon; Jung, Seung-Hyeon; Cho, Kyung-Hyun

2011-03-01

Culinary herbs and spices have been widely used for their hypoglycemic, lipid-lowering, and anti-inflammatory activities. This study examined the physiologic activity of hydrophilic components using extracts of turmeric or laurel leaf powder. Aqueous extracts of turmeric and laurel showed potent inhibitory activity against fructose-mediated glycation with antioxidant ability against low-density lipoprotein (LDL) oxidation and radical scavenging activity. The turmeric and laurel extracts had potent cholesteryl ester transfer protein (CETP) inhibitory ability (up to 23% and 40% inhibition, respectively) at a final concentration of 10 μg/mL. The turmeric and laurel extracts inhibited the cellular uptake of oxidized LDL into macrophages, which is the initial step in atherogenesis. For in vivo testing, zebrafish consumed a high cholesterol diet (HCD) (final concentration, 4% [wt/wt]) with or without turmeric or laurel powder (final concentration, 10% [wt/wt]). The turmeric and laurel groups had a 14% and 12% decrease, respectively, in the weight and height ratios compared to the HCD group. The plasma total cholesterol level was significantly lower in the turmeric and laurel groups (48% and 28% less, respectively, than in the HCD group). Plasma triglycerides were more markedly reduced in the turmeric and laurel groups than in the HCD group (68% and 56% less, respectively, than the HCD group). In conclusion, the hydrophilic extracts of turmeric and laurel potently suppressed the incidence of atherosclerosis via a strong antioxidant potential, prevention of apolipoprotein A-I glycation and LDL phagocytosis, and inhibition of CETP. Consumption of turmeric and laurel extracts exhibited hypolipidemic and antioxidant activities in a hypercholesterolemic zebrafish model.

Ecological implications of Laurel Wilt infestation on Everglades Tree Islands, southern Florida

Science.gov (United States)

Snyder, James R.

2014-01-01

There is a long history of introduced pests attacking native forest trees in the United States (Liebhold and others, 1995; Aukema and others, 2010). Well-known examples include chestnut blight that decimated the American chestnut (Castanea dentata), an extremely important tree in the eastern United States, both as a food source for wildlife and humans and for the wood; Dutch elm disease that attacks native elms (Ulmus spp.), including those commonly planted as shade trees along city streets; and the balsam wooly adelgid (Adelges piceae), an insect that is destroying Fraser firs (Abies fraseri) in higher elevations of Great Smoky Mountains National Park. Laurel wilt, a fungal disease transmitted by the redbay ambrosia beetle (Xyleborus glabratus), is a 21st-century example of an introduced forest pest that attacks native tree species in the laurel family (Lauraceae) (Mayfield, 2007; Hulcr and Dunn, 2011).The introduction of laurel wilt disease has been traced to the arrival of an Asian ambrosia beetle (Xyleborus glabratus) at Port Wentworth, Georgia, near Savannah, in 2002, apparently accidently introduced in wooden shipping material (Mayfield, 2007). Within the next 2 years, it was determined that the non-native wood-boring insect was the vector of an undescribed species of fungus, responsible for killing large numbers of red bay (Persea borbonia) trees in the surrounding area. Dispersing female redbay ambrosia beetles drill into live trees and create tunnels in the wood. They carry with them fungal spores in specialized organs called mycangia at the base of each mandible and sow the spores in the tunnels they excavate. The fungus, since named Raffaelea lauricola (Harrington and others, 2008), is the food source for adults and larvae. The introduction of Raffaelea lauricola causes the host plant to react in such a way as to block the vascular tissue, resulting in loss of water conduction, wilt, and death (Kendra and others, 2013).Although first seen in red bay

National Dam Inspection Program. Laurel Run Dam. NDI ID Number PA-00380. DER ID Number 35-6, Pennsylvania Gas and Water Company. Susquehanna River Basin, Laurel Run, Lackawanna County, Pennsylvania Phase I Inspection Report,

Science.gov (United States)

1980-04-01

Supply. g. Design and Construction History. Laurel Run Dam was constructed in 1594 by Martin Cawley, a contractor from Archbald. The construction was...1T6Ace joly PHASE I INSPECTION REPORT -4 NATIONAL DAM INSPECTION PROGRAM Lime LAUREL RUN DAM PENNSYLVANIA GAS AND WATER COMPANY RESERVOIR AREA

Human Stressors Are Driving Coastal Benthic Long-Lived Sessile Fan Mussel Pinna nobilis Population Structure More than Environmental Stressors.

Directory of Open Access Journals (Sweden)

Salud Deudero

Full Text Available Coastal degradation and habitat disruption are severely compromising sessile marine species. The fan shell Pinna nobilis is an endemic, vulnerable species and the largest bivalve in the Mediterranean basin. In spite of species legal protection, fan shell populations are declining. Models analyzed the contributions of environmental (mean depth, wave height, maximum wave height, period of waves with high energy and mean direction of wave source versus human-derived stressors (anchoring, protection status, sewage effluents, fishing activity and diving as explanatory variables depicting Pinna nobilis populations at a mesoscale level. Human stressors were explaining most of the variability in density spatial distribution of fan shell, significantly disturbing benthic communities. Habitat protection affected P. nobilis structure and physical aggression by anchoring reveals a high impact on densities. Environmental variables instead played a secondary role, indicating that global change processes are not so relevant in coastal benthic communities as human-derived impacts.

Phytophthora ramorum does not cause physiologically significant systemic injury to California bay laurel, its primary reservoir host.

Science.gov (United States)

DiLeo, M V; Bostock, R M; Rizzo, D M

2009-11-01

California bay laurel trees (Umbellularia californica) play a crucial role in the reproduction and survival of Phytophthora ramorum in coastal California forests by supporting sporulation during the rainy season and by providing a means for the pathogen to survive the dry, Mediterranean summer. While bay laurel is thus critical to the epidemiology of sudden oak death and other P. ramorum diseases in California, the relatively minor symptoms observed on this reservoir host suggest that it may not sustain ecologically significant injury itself. The long-term role that P. ramorum will play in California forests will depend in part on the extent to which this pathogen decreases the ecological fitness of bay laurel. Despite the importance of this question, no study has yet investigated in detail the physiological impact that ramorum blight imposes on bay laurel. This experimental study quantifies the impact that P. ramorum has on artificially inoculated bay laurel seedlings with measurements that integrate the full injury that infection with an oomycete may cause: photosynthetic efficiency, total photosynthetic area, and growth. Leaf area and leaf mass were not impacted significantly by infection of P. ramorum. Photosynthetic efficiency was mildly depressed in symptomatic, but not asymptomatic leaves, despite unnaturally high levels of necrosis that were imposed on the seedlings. These results demonstrate that bay laurel trees suffer only minor injury from ramorum blight beyond visible necrotic symptoms. Consequently, it is highly likely that bay laurel will continue to be widely available as a host for P. ramorum in California forests, which has long-term implications for the composition of these forests.

California bay laurel susceptibility to Phythophthora ramorum depends upon season, leaf age, and fungal load

Science.gov (United States)

Steve Johnston; Nathan Rank; Michael Cohen; Ross Meentemeyer

2010-01-01

Phytophthora ramorum can produce spores on dozens of native California plant species, but the most important vector for infection of oak (Quercus) is California bay laurel (Umbellularia californica). Presence of bay laurel is associated with increased infection of oaks and it is the most common tree...

First report of laurel wilt, caused by Raffaelea lauricola , on Sassafras ( Sassafras albidum ) in Arkansas

Science.gov (United States)

Rabiu Olatinwo; C. Barton; Stephen Fraedrich; W. Johnson; J. Hwang

2016-01-01

Laurel wilt, caused by Raffaelea lauricola T.C. Harrin., Aghayeva & Fraedrich, is a lethal disease that affects members of the laurel family (Lauraceae) including avocado (Persea americana Mill), redbay (Persea borbonia [L.] Spreng., Nees), and sassafras (Sassafras albidum [Nutt.]). The fungus is a symbiont of an exotic ambrosia beetle (Xyleborus glabratus...

Laurel wilt: A global threat to avocado production

Science.gov (United States)

Laurel wilt kills American members of the Lauraceae plant family, including avocado (Persea americana). The disease threatens commercial avocado production in Florida, as well as the National Germplasm Repository for avocado in Miami (USDA-ARS). Elsewhere in the US, major (California) and minor comm...

75 FR 70742 - AES Laurel Mountain, LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes...

Science.gov (United States)

2010-11-18

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. ER11-2036-000] AES Laurel Mountain, LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request for Blanket... proceeding of AES Laurel Mountain, LLC's application for market-based rate authority, with an accompanying...

Effects of prolonged drought on stem non-structural carbohydrates content and post-drought hydraulic recovery in Laurus nobilis L.: The possible link between carbon starvation and hydraulic failure.

Science.gov (United States)

Trifilò, Patrizia; Casolo, Valentino; Raimondo, Fabio; Petrussa, Elisa; Boscutti, Francesco; Lo Gullo, Maria Assunta; Nardini, Andrea

2017-11-01

Drought-induced tree decline is a complex event, and recent hypotheses suggest that hydraulic failure and carbon starvation are co-responsible for this process. We tested the possible role of non-structural carbohydrates (NSC) content on post-drought hydraulic recovery, to verify the hypothesis that embolism reversal represents a mechanistic link between carbon starvation and stem hydraulics. Measurements were performed in laurel plants subjected to similar water stress levels either over short or long term, to induce comparable embolism levels. Plants subjected to mild and prolonged water shortage (S) showed reduced growth, adjustment of turgor loss point driven by changes in both osmotic potential at full turgor and bulk modulus of elasticity, a lower content of soluble NSC and a higher content of starch with respect to control (C) plants. Moreover, S plants showed a lower ability to recover from xylem embolism than C plants, even after irrigation. Our data suggest that plant carbon status might indirectly influence plant performance during and after drought via effects on xylem hydraulic functioning, supporting the view of a possible mechanistic link between the two processes. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Green Extraction of Natural Antioxidants from the Sterculia nobilis Fruit Waste and Analysis of Phenolic Profile

Directory of Open Access Journals (Sweden)

Jiao-Jiao Zhang

2018-05-01

Full Text Available The waste of Sterculia nobilis fruit was massively produced during food processing, which contains lots of natural antioxidants. In this study, antioxidants in the Sterculia nobilis fruit waste were extracted using the green microwave-assisted extraction (MAE technique. The effects of five independent variables (ethanol concentration, solvent/material ratio, extraction time, temperature, and microwave power on extraction efficiency were explored, and three major factors (ethanol concentration, extraction time, and temperature showing great influences were chosen to study their interactions by response surface methodology. The optimal conditions were as follows: 40.96% ethanol concentration, 30 mL/g solvent/material ratio, 37.37 min extraction time at 66.76 °C, and 700 W microwave power. The Trolox equivalent antioxidant capacity value obtained in optimal conditions was in agreement with the predicted value. Besides, MAE improved the extraction efficiency compared with maceration and Soxhlet extraction methods. Additionally, the phenolic profile in the extract was analyzed by UPLC-MS/MS, and eight kinds of phenolic compounds were identified and quantified, including epicatechin, protocatechuic acid, ferulic acid, gallic acid, p-coumaric acid, caffeic acid, quercetin, and p-hydroxycinnamic acid. This study could contribute to the value-added utilization of the waste from Sterculia nobilis fruit, and the extract could be developed as food additive or functional food.

Antiviral effect of Anthocleista nobilis root extract on the liver homogenate indices of poultry fowls infected with Newcastle Disease Virus (NDV)

OpenAIRE

Ayodele P. O,; Okonko I. O,; Chukwuka K. S,; Odu N. N; Michael V. I

2011-01-01

This study reports the preliminary investigation of the antiviral effect of Anthocleista nobilis root extract on the liver homogenate indices of poultry fowls treated for Newcastle Disease (ND). Eighteen (18) weeks-old fowls were used for this study. These were divided into 3 groups, A (infected and with treatment), B (infected and without treatment) and C (control). Groups A and B were challenged with Newcastle disease virus (NDV). Group A and C were given ethanolic root extract of A. nobili...

77 FR 47625 - Laurel Hill Wind Energy, LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes...

Science.gov (United States)

2012-08-09

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. ER12-2313-000] Laurel Hill Wind Energy, LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request For... Laurel Hill Wind Energy, LLC's application for market-based rate authority, with an accompanying rate...

78 FR 47317 - Ore Knob Mine Superfund Site; Laurel Springs, Ashe County, North Carolina; Notice of Settlement

Science.gov (United States)

2013-08-05

... ENVIRONMENTAL PROTECTION AGENCY [FRL-9843-3; CERCLA-04-2013-3759] Ore Knob Mine Superfund Site; Laurel Springs, Ashe County, North Carolina; Notice of Settlement AGENCY: Environmental Protection Agency... settlement with Herbert N. Francis concerning the Ore Knob Mine Superfund Site located in Laurel Springs...

Mitochondrial DNA reveals genetic structuring of Pinna nobilis across the Mediterranean Sea.

Directory of Open Access Journals (Sweden)

Daria Sanna

Full Text Available Pinna nobilis is the largest endemic Mediterranean marine bivalve. During past centuries, various human activities have promoted the regression of its populations. As a consequence of stringent standards of protection, demographic expansions are currently reported in many sites. The aim of this study was to provide the first large broad-scale insight into the genetic variability of P. nobilis in the area that encompasses the western Mediterranean, Ionian Sea, and Adriatic Sea marine ecoregions. To accomplish this objective twenty-five populations from this area were surveyed using two mitochondrial DNA markers (COI and 16S. Our dataset was then merged with those obtained in other studies for the Aegean and Tunisian populations (eastern Mediterranean, and statistical analyses (Bayesian model-based clustering, median-joining network, AMOVA, mismatch distribution, Tajima's and Fu's neutrality tests and Bayesian skyline plots were performed. The results revealed genetic divergence among three distinguishable areas: (1 western Mediterranean and Ionian Sea; (2 Adriatic Sea; and (3 Aegean Sea and Tunisian coastal areas. From a conservational point of view, populations from the three genetically divergent groups found may be considered as different management units.

Proteome and Transcriptome Analysis of Ovary, Intersex Gonads, and Testis Reveals Potential Key Sex Reversal/Differentiation Genes and Mechanism in Scallop Chlamys nobilis.

Science.gov (United States)

Shi, Yu; Liu, Wenguang; He, Maoxian

2018-04-01

Bivalve mollusks exhibit hermaphroditism and sex reversal/differentiation. Studies generally focus on transcriptional profiling and specific genes related to sex determination and differentiation. Few studies on sex reversal/differentiation have been reported. A combination analysis of gonad proteomics and transcriptomics was conducted on Chlamys nobilis to provide a systematic understanding of sex reversal/differentiation in bivalves. We obtained 4258 unique peptides and 93,731 unigenes with good correlation between messenger RNA and protein levels. Candidate genes in sex reversal/differentiation were found: 15 genes differentially expressed between sexes were identified and 12 had obvious sexual functions. Three novel genes (foxl2, β-catenin, and sry) were expressed highly in intersex individuals and were likely involved in the control of gonadal sex in C. nobilis. High expression of foxl2 or β-catenin may inhibit sry and activate 5-HT receptor and vitellogenin to maintain female development. High expression of sry may inhibit foxl2 and β-catenin and activate dmrt2, fem-1, sfp2, sa6, Amy-1, APCP4, and PLK to maintain male function. High expression of sry, foxl2, and β-catenin in C. nobilis may be involved in promoting and maintaining sex reversal/differentiation. The downstream regulator may not be dimorphic expressed genes, but genes expressed in intersex individuals, males and females. Different expression patterns of sex-related genes and gonadal histological characteristics suggested that C. nobilis may change its sex from male to female. These findings suggest highly conserved sex reversal/differentiation with diverged regulatory pathways during C. nobilis evolution. This study provides valuable genetic resources for understanding sex reversal/differentiation (intersex) mechanisms and pathways underlying bivalve reproductive regulation.

First report of laurel wilt, caused by Raffaelea lauricola , on spicebush ( Lindera benzoin ) in South Carolina

Science.gov (United States)

S. W. Fraedrich; T. C. Harrington; B. A. McDaniel; G. S. Best

2016-01-01

Most members of the laurel family (Lauraceae) indigenous to the United States appear to be highly susceptible to laurel wilt, a disease caused by Raffaelea lauricola T.C. Harr., Aghayeva & Fraedrich, a fungal symbiont of the redbay ambrosia beetle (Xyleborus glabratus Eichhoff) (Fraedrich et al. 2008; Harrington et al. 2008). The beetle and fungus were...

Determination of volatiles produced during radiation processing in Laurus cinnamomum

Energy Technology Data Exchange (ETDEWEB)

Salum, D.C.; Araujo, M.M.; Fanaro, G.B. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Cidade Universitaria, Av. Professor Lineu Prestes 2242, Zip code: 05508-000 Butanta, Sao Paulo, SP (Brazil); Purgatto, E. [Faculdade de Ciencias Farmaceuticas, FCF/USP, Departamento de Alimentos e Nutricao Experimental. Av. Prof. Lineu Prestes, 580 Bloco 14. CEP: 05508-900 Sao Paulo, SP (Brazil)], E-mail: epurgatt@usp.br; Villavicencio, A.L.C.H. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Cidade Universitaria, Av. Professor Lineu Prestes 2242, Zip code: 05508-000 Butanta, Sao Paulo, SP (Brazil)], E-mail: villavic@ipen.br

2009-07-15

In order to protect food from pathogenic microorganisms as well as increase its shelf-life, while keeping sensorial properties (e.g., odor and taste), which are important properties required by spice buyers, it is necessary to analyze volatile formation from irradiation of medicinal and food herbs. Possible changes in the odor of these herbs are evaluated by characterizing different radiation doses and effects on sensorial properties, in order to allow better application of the irradiation technology. The aim of the present study was to analyze volatile formation on cinnamon (Laurus cinnamomum) samples after gamma irradiation. These samples were irradiated into plastic packages using a {sup 60}Co facility. Radiation doses applied were 0, 5, 10, 15, 20 and 25 kGy. For the analysis of the samples, solid-phase microextraction (SPME) was applied, while for the analysis of volatile compounds, CG/MS. Spice irradiation showed the highest decrease in volatile compounds. For L. cinnamomum, the irradiation decreased volatile compounds by nearly 56% and 89.5%, respectively, comparing to volatile from a sample which had not been previously irradiated.

Antioxidant, Anti-inflammatory, Analgesic Properties, and Phytochemical Characterization of Stem Bark Extract and Fractions of Anthocleista nobilis.

Science.gov (United States)

Ngwoke, Kenneth Gerald; Akwagbulam, Amaka Godsaveus; Erhirhie, Ernest Oghenesuvwe; Ajaghaku, Daniel Lotanna; Okoye, Festus Basden Chiedu; Esimone, Charles Okechukwu

2018-01-01

Anthocleista nobilis ( Loganiaceae ) is used by Mbano people of Imo State, Nigeria, for the treatment of various ailments. The aim of this study is to evaluate the antioxidant, anti-inflammatory, and analgesic properties of the methanol extract, fractions, and subfractions of A. nobilis . The powdered stem bark was extracted with methanol and sequentially fractionated into n-hexane, ethyl acetate, and butanol fractions. The constituents of the fractions were analyzed using high-pressure liquid chromatography (HPLC), and the components were identified by dereplication. Antioxidant potential of the extracts and fractions was investigated using 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging method. Anti-inflammatory and analgesic activities of the extract and fractions were also investigated using xylene-induced inflammation and acetic acid-induced writhing models, respectively. A total of five compounds isovitexin ( R t = 18.77 min), isovitexin-2''-O-xyl ( R t = 19.68 min), p-Hydroxybenzoic acid ( R t = 11.88 min), Sarasinoside L ( R t = 19.64 min), isovitexin ( R t = 18.77), and apigenin monoglycoside ( R t = 19.64 min) were identified by HPLC analysis and dereplication. The ethyl acetate fraction and subfraction elicited the best anti-inflammatory activity. The ethyl acetate subfraction also inhibited acetic acid-induced pain by 79% and 85.0% at the doses of 100 mg/kg and 200 mg/kg, respectively, which was better than 71.1% and 81.3% observed for diclofenac at similar doses. A. nobilis could be a potential source of anti-inflammatory and analgesic lead compounds. The extract, fractions and subfractions of Anthocleista nobilis were screened or antioxidant, anti-inflammatory and Analgesic properties in vitro and in mice models. Some of the components were identified by dereplication after HPLC analysis. The results demonstrated potent anti-inflammatory and analgesic property of the extracts and fractions. The dereplication analysis also identified vitexin and

Antioxidant, Anti-inflammatory, Analgesic Properties, and Phytochemical Characterization of Stem Bark Extract and Fractions of Anthocleista nobilis

Science.gov (United States)

Ngwoke, Kenneth Gerald; Akwagbulam, Amaka Godsaveus; Erhirhie, Ernest Oghenesuvwe; Ajaghaku, Daniel Lotanna; Okoye, Festus Basden Chiedu; Esimone, Charles Okechukwu

2018-01-01

Background: Anthocleista nobilis (Loganiaceae) is used by Mbano people of Imo State, Nigeria, for the treatment of various ailments Objective: The aim of this study is to evaluate the antioxidant, anti-inflammatory, and analgesic properties of the methanol extract, fractions, and subfractions of A. nobilis. Materials and Methods: The powdered stem bark was extracted with methanol and sequentially fractionated into n-hexane, ethyl acetate, and butanol fractions. The constituents of the fractions were analyzed using high-pressure liquid chromatography (HPLC), and the components were identified by dereplication. Antioxidant potential of the extracts and fractions was investigated using 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging method. Anti-inflammatory and analgesic activities of the extract and fractions were also investigated using xylene-induced inflammation and acetic acid-induced writhing models, respectively. Results: A total of five compounds isovitexin (Rt = 18.77 min), isovitexin-2''-O-xyl (Rt = 19.68 min), p-Hydroxybenzoic acid (Rt = 11.88 min), Sarasinoside L (Rt = 19.64 min), isovitexin (Rt = 18.77), and apigenin monoglycoside (Rt = 19.64 min) were identified by HPLC analysis and dereplication. The ethyl acetate fraction and subfraction elicited the best anti-inflammatory activity. The ethyl acetate subfraction also inhibited acetic acid-induced pain by 79% and 85.0% at the doses of 100 mg/kg and 200 mg/kg, respectively, which was better than 71.1% and 81.3% observed for diclofenac at similar doses. Conclusion: A. nobilis could be a potential source of anti-inflammatory and analgesic lead compounds. SUMMARY The extract, fractions and subfractions of Anthocleista nobilis were screened or antioxidant, anti-inflammatory and Analgesic properties in vitro and in mice models. Some of the components were identified by dereplication after HPLC analysis. The results demonstrated potent anti-inflammatory and analgesic property of the extracts and

SOME COMPARATIVE BIOMETRIC ASPECTS IN ARISTICHTHYS NOBILIS AND HYPOPHTHALMICHTHYS MOLITRIX

Directory of Open Access Journals (Sweden)

GABRIELA VASILE

2008-10-01

Full Text Available The paper performs a comparative analysis of some biometric (total length, standard length, head length and caudal footstalks length in two cultured cyprinids species grown in a controlled system, namely: Aristichthys nobilis (bighead carp and Hypophthalmichthys molitrix (silver carp, of various ages (starting with the first up to the fourth growth summer. The results obtained evidenced that, along the four growing stages, the representatives of Hypophthalmichthys molitrix show slightly higher values than those of Aristichthys genus for the total and standard bodily length, while for the head length the situation is reversed.

A bittersweet story: the true nature of the laurel of the Oracle of Delphi.

Science.gov (United States)

Harissis, Haralampos V

2014-01-01

It is known from ancient sources that "laurel," identified with sweet bay, was used at the ancient Greek oracle of Delphi. The Pythia, the priestess who spoke the prophecies, purportedly used laurel as a means to inspire her divine frenzy. However, the clinical symptoms of the Pythia, as described in ancient sources, cannot be attributed to the use of sweet bay, which is harmless. A review of contemporary toxicological literature indicates that it is oleander that causes symptoms similar to those of the Pythia, while a closer examination of ancient literary texts indicates that oleander was often included under the generic term laurel. It is therefore likely that it was oleander, not sweet bay, that the Pythia used before the oracular procedure. This explanation could also shed light on other ancient accounts regarding the alleged spirit and chasm of Delphi, accounts that have been the subject of intense debate and interdisciplinary research for the last hundred years.

Juvenile Pen Shells (Pinna nobilis) Tolerate Acidification but Are Vulnerable to Warming

KAUST Repository

Basso, Lorena

2015-02-25

In the course of this century, rising anthropogenic CO2 emissions will likely cause a decrease in ocean pH, know as ocean acidification, together with an increase of water temperature. Only in the last years, studies have focused on synergetic effects of both stressors on marine invertebrates, particularly on early life stages considered more vulnerable. Disparate responses of their singular and combined effects were reported, highlighting the importance of extending the studies to different species and populations of marine invertebrates. Here, we observed the response of important parameters such as growth, mortality and oxygen consumption of juvenile pen shell Pinna nobilis at supplied pCO2 gas levels of 400 ppm (ambient) and 1000 ppm and at three temperatures (20, 23 and 26 °C) during 36 days. To our knowledge, this is the first study on ocean acidification and temperature effects on juveniles of this species. We show that the two stressors play roles at distinct levels, with pCO2 influencing growth and partially mortality, and temperature increasing mortality rates and oxygen consumption strongly. Therefore, juveniles of P. nobilis are more likely affected by increasing temperature than the pCO2 levels expected by the end of the twenty-first century.

Anti-mycobacterial activity of polyketides from Penicillium sp. endophyte isolated from Garcinia nobilis against Mycobacterium smegmatis

Directory of Open Access Journals (Sweden)

Jean Bosco Jouda

2016-01-01

Conclusion: Isolated compounds from Penicillium sp. harbored in G. nobilis exhibited promising antimycobacterial activity against M. smegmatis thus supporting the immensity of the potential of antimycobacterial drug discovery from endophytes from medicinal plants. Penialidin C could further be investigated for antimycobacterial drug development.

An Endophytic Actinomycete,Sterptomyces sp.AOK-30,Isolated from Mountain Laurel and Its Antifungal Activity

OpenAIRE

Tomio, NISHIMURA; Akane, MEGURO; Sachiko, HASEGAWA; Yoshiko, NAKAGAWA; Masafumi, SHIMIZU; Hitoshi, KUNOH; Akatsuka Orchid Co.,Ltd.; Akatsuka Orchid Co.,Ltd.; Akatsuka Orchid Co.,Ltd.; Akatsuka Orchid Co.,Ltd.; Laboratory of Ecological Circulation,Faculty of Bioresources,Mie University; Laboratory of Ecological Circulation,Faculty of Bioresources,Mie University

2002-01-01

To survey endophytic actinomycetes as potential biocontrol agents against fungal diseases of mountain laurel, young plants of mountain laurel (Kalmia latifolia L.) were used as an isolation source. From a total of 73 actinomycetes isolates obtained from leaves, stems and roots of test plants, Streptomyces sp. AOK-30 was selected, because i) it had a broad and intense antimicrobial spectrum against various yeasts and fungal pathogens of Ericaceae, ii) it grew on the multiplication and rooting ...

Habitat use by a large population of Pinna nobilis in shallow waters

Directory of Open Access Journals (Sweden)

Patricia Prado

2014-12-01

Full Text Available We investigated the habitat use and size structure of the fan mussel, Pinna nobilis L. in the Alfacs Bay (Ebro Delta, Spain, NW Mediterranean. Shore-parallel transects were conducted to assess the abundance, size, and orientation of individuals and to record habitat features along the Banya Sandspit, at depths of 20 to 130 cm. Results showed two distinctive areas in terms of population density, marked by the end of local salt pan dikes that we named Good Habitat (GH and Bad Habitat (BH. To extrapolate these results to the full area of BH and GH, perpendicular transects were conducted to determine the local bathymetry. Then, the underestimation of individuals due to the effects of distance, depth and seagrass cover (Distance 6 computer package was added to raw abundances to obtain a corrected population of 90303 individuals (12085 in the BH and 782018 in the GH, the largest so far reported at such low depths. Most recorded individuals were adults (40 to 60 cm shell length, with no occurrence of sizes < 20 cm, and with a strong association with Cymodocea nodosa seagrass beds. Given the shallow distribution of the population, the absence of small sizes during the study period, and the presence of human activities that may damage P. nobilis and its habitat, the development of a management plan appears imperative for the conservation of the species.

78 FR 62614 - Guttman Energy, Inc., PBF Holding Company LLC v. Buckeye Pipe Line Company, L.P., Laurel Pipe...

Science.gov (United States)

2013-10-22

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. OR14-4-000] Guttman Energy, Inc., PBF Holding Company LLC v. Buckeye Pipe Line Company, L.P., Laurel Pipe Line Company, L.P... complaint against Buckeye Pipe Line Company L.P. and Laurel Pipe Line Company L.P. (Respondents) challenging...

Native and introduced gastropods in laurel forests on Tenerife, Canary Islands

Science.gov (United States)

Kappes, Heike; Delgado, Juan D.; Alonso, María R.; Ibáñez, Miguel

2009-09-01

The introduction of non-native gastropods on islands has repetitively been related to a decline of the endemic fauna. So far, no quantitative information is available even for the native gastropod fauna from the laurel forests (the so-called Laurisilva) of the Canary Islands. Much of the original laurel forest has been logged in recent centuries. Based on vegetation studies, we hypothesized that densities and the number of introduced species decline with the age of the regrowth forests. We sampled 27 sites from which we collected thirty native and seven introduced species. Two introduced species, Milax nigricans and Oxychilus alliarius, were previously not reported from the Canary Islands. Assemblage composition was mainly structured by disturbance history and altitude. Overall species richness was correlated with slope inclination, prevalence of rocky outcrops, amounts of woody debris and leaf litter depth. Densities were correlated with the depth of the litter layer and the extent of herb layer cover and laurel canopy cover. Introduced species occurred in 22 sites but were neither related to native species richness nor to the time that elapsed since forest regrowth. One introduced slug, Lehmannia valentiana, is already wide-spread, with densities strongly related to herb cover. Overall species richness seemed to be the outcome of invasibility, thus factors enhancing species richness likely also enhance invasibility. Although at present introduced species contribute to diversity, the potential competition between introduced slugs and the rich native semi-slug fauna, and the effects of introduced predatory snails ( Oxychilus spp. and Testacella maugei) warrant further monitoring.

An evaluation of seven methods for controlling mountain laurel thickets in the mixed-oak forests of the central Appalachian Mountains, USA

Science.gov (United States)

Patrick H. Brose

2017-01-01

In the Appalachian Mountains of eastern North America, mountain laurel (Kalmia latifolia) thickets in mixed-oak (Quercus spp.) stands can lead to hazardous fuel situations, forest regeneration problems, and possible forest health concerns. Therefore, land managers need techniques to control mountain laurel thickets and limit...

Trichodina nobilis Chen, 1963 and Trichodina reticulata Hirschmann et Partsch, 1955 from ornamental freshwater fishes in Brazil

Directory of Open Access Journals (Sweden)

ML Martins

Full Text Available In the present work Trichodina reticulata and T. nobilis (Ciliophora: Trichodinidae are morphologically characterised from ornamental freshwater fish culture in the State of Santa Catarina, Brazil. The prevalence of infection and a list of comparative measurements are discussed. We examined "southern platyfish" Xiphophorus maculatus (n = 35, "goldfish" Carassius auratus (n = 31, "guppy" Poecilia reticulata (n = 20, "sailfin molly" Poecilia latipinna (n = 6, "beta" Betta splendens (n = 2 and "spotted headstander" Chilodus punctatus (n = 1. After being anesthetised in a benzocaine solution, fishes were examined for parasitological evaluation. A total of 51.57% fishes were parasitised by Trichodina spp. Carassius auratus was the most parasitised species, followed by X. maculatus and P. reticulata. Beta splendens, C. punctatus and P. latipinna were not parasitised by any trichodinid species. Two species of Trichodina were collected from the skin of fish: T. nobilis was found in C. auratus, P. reticulata and X. maculatus and T. reticulata was only observed in C. auratus. The importance of adequate handling in ornamental fish culture are also discussed.

Survival and chlamydospore production of Phytophthora ramorum in California bay laurel leaves

Science.gov (United States)

E. Fichtner; D. Rizzo; S. Lynch; D. Rizzo; G. Buckles; J. Parke

2009-01-01

Sudden oak death manifests as non-lethal foliar lesions on bay laurel (Umbellularia californica), which support sporulation and survival of Phytophthora ramorum in forest ecosystems. The pathogen survives the dry summers in a proportion of attached bay leaves, but the propagules responsible for survival are...

A comparison of complete mitochondrial genomes of silver carp hypophthalmichthys molitrix and bighead carp hypophthalmichthys nobilis: Implications for their taxonomic relationship and phylogeny

Science.gov (United States)

Li, S.-F.; Xu, J.-W.; Yang, Q.-L.; Wang, C.H.; Chen, Q.; Chapman, D.C.; Lu, G.

2009-01-01

Based upon morphological characters, Silver carp Hypophthalmichthys molitrix and bighead carp Hypophthalmichthys nobilis (or Aristichthys nobilis) have been classified into either the same genus or two distinct genera. Consequently, the taxonomic relationship of the two species at the generic level remains equivocal. This issue is addressed by sequencing complete mitochondrial genomes of H. molitrix and H. nobilis, comparing their mitogenome organization, structure and sequence similarity, and conducting a comprehensive phylogenetic analysis of cyprinid species. As with other cyprinid fishes, the mitogenomes of the two species were structurally conserved, containing 37 genes including 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA (tRNAs) genes and a putative control region (D-loop). Sequence similarity between the two mitogenomes varied in different genes or regions, being highest in the tRNA genes (98??8%), lowest in the control region (89??4%) and intermediate in the protein-coding genes (94??2%). Analyses of the sequence comparison and phylogeny using concatenated protein sequences support the view that the two species belong to the genus Hypophthalmichthys. Further studies using nuclear markers and involving more closely related species, and the systematic combination of traditional biology and molecular biology are needed in order to confirm this conclusion. ?? 2009 The Fisheries Society of the British Isles.

Invasion patterns of ground-dwelling arthropods in Canarian laurel forests

Science.gov (United States)

Arndt, Erik; Perner, Jörg

2008-09-01

Patterns of invasive species in four different functional groups of ground-dwelling arthropods (Carnivorous ground dwelling beetles; Chilopoda; Diplopoda; Oniscoidea) were examined in laurel forests of the Canary Islands. The following hypotheses were tested: (A) increasing species richness is connected with decreasing invasibility as predicted by the Diversity-invasibility hypothesis (DIH); (B) disturbed or anthropogenically influenced habitats are more sensitive for invasions than natural and undisturbed habitats; and (C) climatic differences between laurel forest sites do not affect the rate of invasibility. A large proportion of invasives (species and abundances) was observed in most of the studied arthropod groups. However, we did not find any support for the DIH based on the examined arthropod groups. Regarding the impact of the extrinsic factors 'disturbance' and 'climate' on invasion patterns, we found considerable differences between the studied functional groups. Whereas the 'disturbance parameters' played a minor role and only affected the relative abundances of invasive centipedes (positively) and millipedes (negatively), the 'climate parameters' were significantly linked with the pattern of invasive detritivores. Interactions between native and invading species have not been observed thus far, but cannot completely be excluded.

Effects of Gelatin-Based Edible Films Enriched with Laurel Essential Oil on the Quality of Rainbow Trout (Oncorhynchus mykiss Fillets During Refrigerated Storage

Directory of Open Access Journals (Sweden)

Tuba Baygar

2014-01-01

Full Text Available The effects of gelatin films enriched with laurel leaf essential oil on the quality of rainbow trout (Oncorhynchus mykiss during refrigerated storage at (4±1 °C were examined over a period of 26 days. Fish fillets were wrapped with 8 % gelatin films containing 0, 0.1 and 1 % (by volume per mass of laurel essential oil and vacuum packaged. Sensory (raw and cooked fish, microbiological (total viable counts, psychrotrophic bacteria counts, Enterobacteriaceae and lactic acid bacteria and chemical (proximate composition, pH, total volatile base nitrogen (TVB-N, thiobarbituric acid (TBA, free fatty acid (FFA and peroxide value (PV analysis, and colour measurement were carried out during the storage period and shelf-life was determined periodically. The obtained results showed that the gelatin film enriched with laurel essential oil was suitable for the preservation of rainbow trout fillet and the ability of laurel essential oil to preserve the film depended on its ratio. Combined effects of gelatin film and laurel essential oil (1 % by volume per mass was efficient in maintaining the quality characteristics at an acceptable level up to 22 days of storage, while the control and gelatin film without the essential oil reached an unacceptable level at 15 and 20 days, respectively.

Ambrosia beetle communities in forest and agriculture ecosystems with laurel wilt disease

Science.gov (United States)

The invasive redbay ambrosia beetle, Xyleborus glabratus, is an exotic wood-boring pest first detected in 2002 near Savannah, Georgia. The beetle’s dominant fungal symbiont, Raffaelea lauricola, is the pathogen that causes laurel wilt, a lethal disease of trees in the family Lauraceae. Redbay ambro...

Potential Anti-Cancer Activities and Mechanisms of Costunolide and Dehydrocostuslactone

Directory of Open Access Journals (Sweden)

Xuejing Lin

2015-05-01

Full Text Available Costunolide (CE and dehydrocostuslactone (DE are derived from many species of medicinal plants, such as Saussurea lappa Decne and Laurus nobilis L. They have been reported for their wide spectrum of biological effects, including anti-inflammatory, anticancer, antiviral, antimicrobial, antifungal, antioxidant, antidiabetic, antiulcer, and anthelmintic activities. In recent years, they have caused extensive interest in researchers due to their potential anti-cancer activities for various types of cancer, and their anti-cancer mechanisms, including causing cell cycle arrest, inducing apoptosis and differentiation, promoting the aggregation of microtubule protein, inhibiting the activity of telomerase, inhibiting metastasis and invasion, reversing multidrug resistance, restraining angiogenesis has been studied. This review will summarize anti-cancer activities and associated molecular mechanisms of these two compounds for the purpose of promoting their research and application.

Toxicidade de compostos sintéticos e naturais sobre Tetranychus urticae e o predador Phytoseiulus macropilis

Directory of Open Access Journals (Sweden)

Bruce Veronez

2012-04-01

Full Text Available O objetivo deste trabalho foi avaliar a toxicidade de compostos sintéticos e naturais sobre Tetranychus urticae e o predador Phytoseiulus macropilis. A mortalidade e a taxa de crescimento de T. urticae e seu predador foram avaliadas após a aplicação de: abamectina, clofentezina, fenpropatrina, fenpiroximato, propargito, enxofre e espiromesifeno, nas concentrações recomendadas; óleos de nim (Natuneem e Sempre Verde Killer Neem a 1%; e extratos aquosos a 10% de Dieffenbachia brasiliensis, Annona squamosa, Ruta graveolens, Agave angustifolia, Melia azedarach, Sonchus oleraceus, Mentha spicata x M. suaveolens, Allium cepa, Laurus nobilis e Eucalyptus saligna. A toxicidade aguda e a influência dos compostos sobre a taxa de crescimento instantâneo dos ácaros foram avaliadas em laboratório. Extratos de A. cepa, A. angustifolia, produtos à base de óleo de nim, espiromesifeno, propargito, fenpiroximato, abamectina e fenpropatrina causaram mortalidade superior a 83% em T. urticae. Extrato de A. angustifolia, Natuneem e clofentezina não causaram mortalidade significativa em P. macropilis. Agave angustifolia e Natuneem não afetaram significativamente a taxa de crescimento deste predador. Propargito, fenpiroximato, abamectina, fenpropatrina, espiromesifeno e extrato de L. nobilis afetaram severamente a população de P. macropilis.

A COMPARATIVE STUDY ON THE ACTIVITY OF ALANIN-AMINOTRANSFERASE IN HYPOPHTHALMICHTHYS MOLITRIX AND ARISTICHTHYS NOBILIS

Directory of Open Access Journals (Sweden)

Gabriela Vasile

2006-08-01

Full Text Available The present paper represents a comparative study on the activity of one aminotransferase - alaninaminotransferase, in the digestive tube of Hypophthalmichthys molitrix (silver carp and Aristichthys nobilis (bighead carp. The enzymatic activity has been determined colorimetrically, with 2, 4 - dinitrophenyl hydrazine, the results obtained being expressed as UE / g / min. It was observed that, comparatively with the alanin-aminotransferase activity recorded in silver carp, in the case of bighead carp, the values recorded are much lower.

Towards the development of a laurel wilt screening program in redbay (Persea borbonia)

Science.gov (United States)

Marc Hughes; Jason Smith

2012-01-01

Laurel wilt is a highly destructive disease of redbay (Persea borbonia (L.) Spreng.) and other Lauraceous natives in the southeastern United States. The disease and associated vector, the redbay ambrosia beetle (Xyleborus glabratus), has spread through the United States coastal plain. The presence of surviving and...

Two Hour Vocational Classes at the Laurel County Area Vocational School. Final Report.

Science.gov (United States)

Moore, James E.

This developmental project with a research component was initiated at the Laurel County Vocational School with the major objective of determining if 2-hour instructional blocks could be operated in programs enrolling only secondary students. Subobjectives involved data gathering and statistical analyses on (1) student achievement, (2) ratings of…

Using genetic information to inform Redbay restoration in Laurel Wilt epidemic areas

Science.gov (United States)

K.E. Smith; M.A. Hughes; C.S. Echt; S.A. Josserand; C.D. Nelson; J.M. Davis; J.A. Smith

2017-01-01

Laurel wilt disease is incited by the exotic fungus Raffaelea lauricola and transmitted by the Asian redbay ambrosia beetle (Xyleborus glabratus). The disease has spread from Savannah, Georgia in 2002 across the coastal southeast as far south as the Everglades, and in 2014 was discovered as far west as Texas. Mortality is severe, with locations in...

Comparison of ambrosia beetle communities in two hosts with laurel wilt: swampbay vs. avocado

Science.gov (United States)

The invasive redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae), is an exotic wood-boring pest first detected in 2002 near Savannah, Georgia. The beetle’s dominant fungal symbiont, Raffaelea lauricola, is the pathogen that causes laurel wilt, a lethal disease of tre...

Water dynamics in a laurel montane cloud forest in the Garajonay National Park (Canary Islands, Spain)

Science.gov (United States)

García-Santos, G.; Marzol, M. V.; Aschan, G.

Field measurements from February 2003 to January 2004 in a humid (but dry in summer) crest heath wood-land (degraded laurel forest) in the National Park of Garajonay, Canary Islands (Spain), were combined to calculate water balance components. The water balance domain is at the surface of the catchment and is controlled by atmospheric processes and vegetation. This study found that annual water income (rainfall plus fog water) was 1440 mm year-1, half of which was occult (or fog) precipitation, while stand transpiration estimated from measurements of sap flow amounted, annually, to 40% of potential evapotranspiration calculated from measurements of meteorological variables. The positive role of crest laurel forests, which transpire less water than is incoming from rain and fog is emphasised.

Insecticidal effects of essential oils extracted from aromatic plants on Ceratitis capitata (Wied.) in Lebanon

Energy Technology Data Exchange (ETDEWEB)

Abi Chahine, M [Lebanese Agricultural Research Institute, Jdeidet el-Metn (Lebanon); Khoury, N; Webeh, E [Lebanese Agricultural Research Institute, Jdeidet el-Metn (Lebanon)

2005-07-01

Full text: The excessive use of chemical pesticides to control agricultural pests is becoming alarming. The objective of this study is to search for biopesticides of plant origin that could be used to control one of the major pest of fruit production; the Mediterranean fruit fly (Ceratitis capitata Wied.). A colony of the Lebanese wild strain of this insect was reared under laboratory condition to provide biological material. The insecticidal activity of the essential oils extracted from aromatic plants in Lebanon was assessed. The tested plants are: Foeniculum vulgare, Thymbra spicata, Artemisia herba alba, Origanum syriacum, Ruta chalepensis, Lavandula stoechas, Salvia fruticosa, Mentha microphylla, Juniperus oxycedrus, Rosmarinus officinalis, Myrtus communis, Laurus nobilis and Ocimum gratissimum. Results show that essential oils isolated from F. vulgare, T. spicata, A. herba alba, O. syriacum and R. chalepensis have promising insecticidal potential. (author)

Preliminary study of the molluscicidal and larvicidal properties of some essential oils and phytochemicals from medicinal plants

Directory of Open Access Journals (Sweden)

Aristides M. Leite

Full Text Available This study aimed to evaluate the molluscicidal and larvicidal activity of some essential oils and phytochemicals from medicinal plants. Molluscicide and larvicidal activity were determined by, respectively, the lethality bioassays using Artemia salina Leach. Artemiidae and Aedes aegypti L. Culicidae larvae. Essential oils from Eugenia uniflora L. Myrtaceae, Laurus nobilis L. Lauraceae, Origanum vulgare L. Lamiaceae and the phytochemicals α-pinene and eugenol presented citotoxicity toward Artemia salina with CL50 values between 9.59 and 253.43 μL/mL. Essential oils from E. uniflora, M. piperita, O. vulgare and R. officinalis showed embryotoxicity on Aedes aegypti larvae with a viability inhibition between 40 and 100%. These results show the bioactivity of the assayed essential oils and phytochemicals and, partially, justify their insertion in further evaluation in order to establish a safe exploitation of their biological potentiality.

Laurel Wilt in Natural and Agricultural Ecosystems: Understanding the Drivers and Scales of Complex Pathosystems

Directory of Open Access Journals (Sweden)

Randy C. Ploetz

2017-02-01

Full Text Available Laurel wilt kills members of the Lauraceae plant family in the southeastern United States. It is caused by Raffaelea lauricola T.C. Harr., Fraedrich and Aghayeva, a nutritional fungal symbiont of an invasive Asian ambrosia beetle, Xyleborus glabratus Eichhoff, which was detected in Port Wentworth, Georgia, in 2002. The beetle is the primary vector of R. lauricola in forests along the southeastern coastal plain of the United States, but other ambrosia beetle species that obtained the pathogen after the initial introduction may play a role in the avocado (Persea americana Miller pathosystem. Susceptible taxa are naïve (new-encounter hosts that originated outside Asia. In the southeastern United States, over 300 million trees of redbay (P. borbonia (L. Spreng. have been lost, and other North American endemics, non-Asian ornamentals and avocado—an important crop that originated in MesoAmerica—are also affected. However, there are no reports of laurel wilt on the significant number of lauraceous endemics that occur in the Asian homeland of R. lauricola and X. glabratus; coevolved resistance to the disease in the region has been hypothesized. The rapid spread of laurel wilt in the United States is due to an efficient vector, X. glabratus, and the movement of wood infested with the insect and pathogen. These factors, the absence of fully resistant genotypes, and the paucity of effective control measures severely constrain the disease’s management in forest ecosystems and avocado production areas.

First report of Laurel Wilt caused by Rafffaelea lauricola on sassafras in Mississippi

Science.gov (United States)

J.J. Riggins; S.W. Fraedrich; T.C. Harrington

2011-01-01

Laurel wilt is caused by the fungus Raffaelea lauricola T.C. Harrin., Aghayeva & Fraedrich and is lethal to redbay (Persea borbonia (L.) Spreng.), sassafras (Sassafras albidum (Nutt.) Nees), and other species in the Lauraceae (1). The fungus is carried by the redbay ambrosia beetle (Xyleborus glabratus Eichh.), which is native to Asia.

El trampolín tecnológico : el "Incidente Laurel" y España en la II Guerra Mundial

OpenAIRE

Florentino Rodao

1994-01-01

El momento más crítico para las relaciones entre España y Estados Unidos durante la II Guerra Mundial fue, con bastante diferencia, el protagonizado por el llamado «Incidente Laurel» en los meses de octubre y noviembre de 1943. El presunto reconocimiento oficial por el ministro de Exteriores Jordana de un gobierno pro-japonés como el de José P. Laurel en Filipinas llevó a una tortísima campaña de prensa contra Franco, combinada con rumores de actuación militar en la Penínsul...

Genetic variation in native populations of the laurel wilt pathogen, Raffaelea lauricola , in Taiwan and Japan and the introduced population in the United States

Science.gov (United States)

Caroline E. Wuest; Thomas C. Harrington; Stephen W. Fraedrich; Hye-Young Yun; Sheng-Shan Lu

2017-01-01

Laurel wilt is a vascular wilt disease caused by Raffaelea lauricola, a mycangial symbiont of an ambrosia beetle, Xyleborus glabratus. The fungus and vector are native to Asia but were apparently introduced to the Savannah, GA, area 15 or more years ago. Laurel wilt has caused widespread mortality on redbay (Persea borbonia) and other members of the Lauraceae in the...

First report of laurel wilt disease caused by Raffaelea lauricola on pondspice in Florida

Science.gov (United States)

M. Hughes; J.A. Smith; A.E. Mayfield III; M.C. Minno; K. Shin

2011-01-01

Laurel wilt is a fungal vascular disease of redbay (Persea borbonia (L.) Spreng) and other plants in the family Lauraceae in the southeastern United States (1). The disease is caused by Raffaelea lauricola T. C. Harr., Fraedrich & Aghayeva, which is vectored by the exotic redbay ambrosia beetle (Xyleborus glabratus...

Juvenile pen shells (Pinna nobilis) tolerate acidification but are vulnerable to warming, supplement to: Basso, L; Hendriks, Iris; Duarte, Carlos M (2015): Juvenile pen shells (Pinna nobilis) tolerate acidification but are vulnerable to warming. Estuaries and Coasts, 38(6), 1976-1985

KAUST Repository

Basso, L

2016-01-01

In the course of this century, rising anthropogenic CO2 emissions will likely cause a decrease in ocean pH, know as ocean acidification, together with an increase of water temperature. Only in the last years, studies have focused on synergetic effects of both stressors on marine invertebrates, particularly on early life stages considered more vulnerable. Disparate responses of their singular and combined effects were reported, highlighting the importance of extending the studies to different species and populations of marine invertebrates. Here, we observed the response of important parameters such as growth, mortality and oxygen consumption of juvenile pen shell Pinna nobilis at supplied pCO2 gas levels of 400 ppm (ambient) and 1000 ppm and at three temperatures (20, 23 and 26 °C) during 36 days. To our knowledge, this is the first study on ocean acidification and temperature effects on juveniles of this species. We show that the two stressors play roles at distinct levels, with pCO2 influencing growth and partially mortality, and temperature increasing mortality rates and oxygen consumption strongly. Therefore, juveniles of P. nobilis are more likely affected by increasing temperature than the pCO2 levels expected by the end of the twenty-first century.

Phosphorus conservation by evergreenness of mountain laurel. [Kalmia latifolia

Energy Technology Data Exchange (ETDEWEB)

Thomas, W A [Oak Ridge National Lab., TN; Grigal, D F

1976-01-01

Field studies and compartmental model analyses demonstrate that the evergreen nature of mountain laurel Kalmia latifolia L. helps conserve phosphorus on infertile sites. The perennial canopy continuously adds P to the forest floor through foliar leaching and year-round leaf fall. Slow mineralization of leaf litter provides a steady addition of available P. The evergreenness of Kalmia allows it to utilize increases in available P, thus keeping it in circulation through biological tissues. Retention of a sufficient P supply in this manner allows the site to respond when changes such as disturbance or succession occur in the system.

The effect of laurel leaf extract against toxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in cultured rat hepatocytes.

Science.gov (United States)

Turkez, Hasan; Geyikoglu, Fatime

2011-12-01

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a very toxic environmental pollutant that raises great public concern about its impact on human health. Recent studies indicate that laurel leaf extract exhibits antioxidant properties that can counter the toxic effects of certain compounds in the liver. The aim of this study was to assess how effective LE is against the toxicity of TCDD in a primary culture of rat hepatocytes. The extract (50 mg L(-1), 100 mg L(-1), and 200 mg L(-1)) was added to cultures alone or with TCDD (1.61 mg L(-1) and 3.22 mg L(-1)) for 48 hours. Cell viability was measured using the [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) assay and the lactate dehydrogenase (LDH) cytotoxicity assay, while oxidative damage was assessed by measuring total antioxidant capacity (TAC) and total oxidative stress (TOS). DNA damage was also analysed using the micronucleus (MN) assay of the cultured hepatocytes. TCDD alone lowered, and laurel extract had no effect on cell viability. TCDD also increased TOS and significantly decreased TAC. It significantly increased the frequency of micronucleated hepatocytes in a dose-dependent manner. In cultures exposed to LE alone, TOS did not change and TAC significantly increased in a dose-dependent manner. Added to TCDD, laurel countered its toxic effects and showed protective effects against TCDD-mediated DNA damage. This points to the therapeutic potential of laurel against TCDD toxicity in the liver.

First report of laurel wilt, caused by Raffaelea lauricola, on sassafras (Sassafras albidum) in Alabama

Science.gov (United States)

C.A. Bates; Stephen Fraedrich; T.C. Harrington; R.S. Cameron; R.D. Menard; Susan Best

2013-01-01

Laurel wilt, caused by Raffaelea lauricola, a fungal symbiont of the redbay ambrosia beetle, Xyleborus glabratus, is responsible for extensive mortality of native redbays (Persea borbonia and P. Palustris) in the coastal plains of the southeastern United States. The wilt also affect the more...

No rest for the laurels: symbiotic invaders cause unprecedented damage to southern USA forests

Science.gov (United States)

M. A. Hughes; J. J. Riggins; F. H. Koch; A. I. Cognato; C. Anderson; J. P. Formby; T. J. Dreaden; R. C. Ploetz; J. A. Smith

2017-01-01

Laurel wilt is an extraordinarily destructive exotic tree disease in the southeastern United States that involves new-encounter hosts in the Lauraceae, an introduced vector (Xyleborus glabratus) and pathogen symbiont (Raffaelea lauricola). USDA Forest Service Forest Inventory and Analysis data were used to estimate that over 300 million trees of redbay (Persea borbonia...

Effects of Gelatin-Based Edible Films Enriched with Laurel Essential Oil on the Quality of Rainbow Trout (Oncorhynchus mykiss) Fillets During Refrigerated Storage

OpenAIRE

Tuba Baygar; Taçnur Baygar; Hatice Hasanhocaoglu; Cansu Metin; Yunus Alparslan

2014-01-01

The effects of gelatin films enriched with laurel leaf essential oil on the quality of rainbow trout (Oncorhynchus mykiss) during refrigerated storage at (4±1) °C were examined over a period of 26 days. Fish fillets were wrapped with 8 % gelatin films containing 0, 0.1 and 1 % (by volume per mass) of laurel essential oil and vacuum packaged. Sensory (raw and cooked fish), microbiological (total viable counts, psychrotrophic bacteria counts, Enterobacteriaceae and lactic acid bacteria) and ...

Host phenology and leaf effects on susceptibility of California bay laurel to Phytophthora ramorum

Science.gov (United States)

Steven F. Johnston; Michael F. Cohen; Tamas Torok; Ross K. Meentemeyer; Nathan E. Rank

2016-01-01

Spread of the plant pathogen Phytophthora ramorum, causal agent of the forest disease sudden oak death, is driven by a few competent hosts that support spore production from foliar lesions. The relationship between traits of a principal foliar host, California bay laurel (Umbellularia californica), and susceptibility to

Quantitative study of fruit flavonoids in citrus hybrids of King (C. nobilis) and Mukaku Kishu (C. kinokuni).

Science.gov (United States)

Kawaii, S; Tomono, Y; Katase, E; Ogawa, K; Nonomura-Nakano, M; Nesumi, H; Yoshida, T; Sugiura, M; Yano, M

2001-08-01

Twenty-four Citrus hybrids of King (C. nobilis) and Mukaku Kishu (C. kinokuni) were examined for their flavonoid profiles of the edible part by reversed-phase HPLC analysis. Two hybrids (G-155 and G-156) contained higher amounts of natsudaidain than their parents, whereas the remainder of the hybrids had a character intermediate between those of King and Mukaku Kishu on the basis of polymethoxylated flavone composition. Principal component analysis revealed the distribution of the hybrids by quantifying 23 flavonoid contents.

Host Phenology and Leaf Effects on Susceptibility of California Bay Laurel to Phytophthora ramorum.

Science.gov (United States)

Johnston, Steven F; Cohen, Michael F; Torok, Tamas; Meentemeyer, Ross K; Rank, Nathan E

2016-01-01

Spread of the plant pathogen Phytophthora ramorum, causal agent of the forest disease sudden oak death, is driven by a few competent hosts that support spore production from foliar lesions. The relationship between traits of a principal foliar host, California bay laurel (Umbellularia californica), and susceptibility to P. ramorum infection were investigated with multiple P. ramorum isolates and leaves collected from multiple trees in leaf-droplet assays. We examined whether susceptibility varies with season, leaf age, or inoculum position. Bay laurel susceptibility was highest during spring and summer and lowest in winter. Older leaves (>1 year) were more susceptible than younger ones (8 to 11 months). Susceptibility was greater at leaf tips and edges than the middle of the leaf. Leaf surfaces wiped with 70% ethanol were more susceptible to P. ramorum infection than untreated leaf surfaces. Our results indicate that seasonal changes in susceptibility of U. californica significantly influence P. ramorum infection levels. Thus, in addition to environmental variables such as temperature and moisture, variability in host plant susceptibility contributes to disease establishment of P. ramorum.

Repellency and toxicity of aromatic plant extracts against the mosquito Culex pipiens molestus (Diptera: Culicidae).

Science.gov (United States)

Traboulsi, Abdallah F; El-Haj, Samih; Tueni, Marie; Taoubi, Khalil; Nader, Natalie Abi; Mrad, Abir

2005-06-01

The insecticidal activities of essential oil extracts from leaves, flowers and roots of aromatic plants against fourth-instar larvae of the mosquito Culex pipiens molestus Forskal were determined. Extracts of Foeniculum vulgare Mill were the most toxic, followed by those of Ferula hermonis Boiss, Citrus sinensis Osbeck, Pinus pinea L, Laurus nobilis L and Eucalyptus spp with LC50 values of 24.5, 44.0, 60.0, 75.0, 117.0 and 120.0 mg litre(-1), respectively. Combination tests between the LC50 and the maximum sub-lethal concentration (MSLC) were determined. Over 20 major components were identified in extracts from each plant species tested. Five essential oils and nine pure components were studied for their repellency against mosquito bites. Terpineol and 1,8-cineole were the most effective against Culex pipiens molestus bites offering complete protection for 1.6 and 2 h, respectively.

Entrapment of laurel lipase in chitosan hydrogel beads.

Science.gov (United States)

Yagar, Hulya; Balkan, Ugur

2017-08-01

Laurel seed lipase was entrapped within chitosan beads with ionotropic gelatin method using tripolyphosphate (TPP) as multivalent covalent counter ion. Immobilization yield was 78%. First, optimum immobilization conditions were determined, and morphology of chitosan beads was characterized by scanning electron microscopy. Optimum pH and temperature were evaluated as 6.0 and 40 °C, respectively. The immobilized beads saved about 55% of its activities at 60° while saved about 32% at 70 °C for 30 min. V max /K m values were determined as 31.75 and 2.87 using olive oil as substrate for immobilized beads and free enzyme, respectively. Immobilized beads showed the activities during 30 days at +4 °C.

Do Affordable Housing Projects Harm Suburban Communities? Crime, Property Values, and Taxes in Mount Laurel, NJ.

Science.gov (United States)

Albright, Len; Derickson, Elizabeth S; Massey, Douglas S

2013-06-01

This paper offers a mixed-method analysis of the municipal-level consequences of an affordable housing development built in suburban New Jersey. Opponents of affordable housing development often suggest that creating affordable housing will harm surrounding communities. Feared consequences include increases in crime, declining property values, and rising taxes. To evaluate these claims, the paper uses the case of Mt. Laurel, NJ - the site of a landmark affordable housing legal case and subsequent affordable housing development. Employing a multiple time series group control design, we compare crime rates, property values, and property taxes in Mt. Laurel to outcomes in similar nearby municipalities that do not contain comparable affordable housing developments. We find that the opening of the affordable housing development was not associated with trends in crime, property values, or taxes, and discuss management practices and design features that may have mitigated potential negative externalities.

Vertical distribution and daily flight periodicity of ambrosia beetles associated with laurel wilt affected avocado orchards.

Science.gov (United States)

Recently, ambrosia beetles have emerged as significant pests of avocado (Persea americana Mill.; Lauraceae) due to their association with fungal pathogens, in particular, the causal agent of laurel wilt disease, Raffaelea lauricola. The objective of this study was to provide insights into the intera...

Reconstruction of water mass properties of the Western Mediterranean using stable isotope ratios (δ18O and δ13C) from Pinna nobilis shells

Science.gov (United States)

Garcia-March, Jose Rafael; Surge, Donna; Tena-Medialdea, Jose; Torres, Javi; Garcia-Martinez, Micaela

2017-04-01

We analysed the stable isotope ratios (δ18O and δ13C) from the calcite of the outer prismatic layer of 8 Pinna nobilis shells at a rate of c. 12-16 samples/year for years between 1997 and 2013. These produced 8 time series between 4 and 16 years long. The empty shells had been collected in two Spanish marine protected areas, Tabarca Island in Alicante and Columbretes Islands in Castellón (4 specimens each). For all shells we constrained the time of death within at least a halve year window. The present study completes previous calibrations by using recorded seawater temperature data during at least two years for each site, coinciding with part of the analysed periods in the shells. Our preliminary results clearly reflect the seasonality of the signal in δ18O values for the entire ontogeny of the individuals studied. However, one specimen from Tabarca Island sampled between years 4 and 11 (corresponding to real age) shows a clear ontogenetic change, with an abrupt reduction in the amplitude of the signal from age 6 to 11 for both δ18O and δ13C values. We hypothesize this pattern relates to the onset of first gonad maturation. Values of δ13C track δ18O values in most individuals, but 3 specimens show low amplitude. For all time series, δ13C values show a decreasing slope with ontogeny similar to previous observations in P. nobilis and other bivalves. Estimated seawater temperature from our δ18O time series reveals the potential of P. nobilis shells as valuable archives of Mediterranean temperature reconstructions in the context of climate change.

First report of laurel wilt, caused by Raffaelea lauricola, on redbay (Persea borbonia) in Texas.

Science.gov (United States)

R. D. Menard; S. R. Clarke; Stephen Fraedrich; T. C. Harrington

2016-01-01

Laurel wilt, caused by Raffaelea lauricola T.C.Harr., Aghayeva, & Fraedrich, a fungal symbiont of the redbay ambrosia beetle (Xyleborus glabratus Eichhoff), is responsible for extensive mortality of redbay (Persea borbonia (L.) Spreng) and other Lauraceae native to the United States (Fraedrich et al. 2008). The beetle and fungus were introduced into the United...

Ecology and morphology of the flowers of Hepatica nobilis Schreb. (Ranunculaceae

Directory of Open Access Journals (Sweden)

Beata Żuraw

2013-04-01

Full Text Available The study was conducted in the years 2009-2010 in the UMCS Botanical Garden in Lublin. The observations involved the flowering time and the seasonal and diurnal dynamics of flowering and pollen production in flowers of the liverleaf (Hepatica nobilis Schreb. from the family Ranunculaceae. The liverleaf begins blooming in early March and the full bloom occurs at the end of March and beginning of April. All-day observations demonstrated that the flowers opened between 8 am and 3 pm, and pollen was released from the anthers between 10 am and 4 pm. The mean weight of pollen produced by 10 liverleaf flowers was estimated at 7.4 mg (1.85 mg/100 stamens. An impact of the weather on the flowering period as well as the seasonal and diurnal dynamics of flowering was observed. Among insect pollinators, the honeybee dominated with a density of 4 individuals per 1m² of a flowerbed that contained 74 open flowers on average.

Palmito de pejibaye (Bactris gasipaes cultivado bajo diferentes densidades de laurel (Cordia alliodora

Directory of Open Access Journals (Sweden)

Johnny Montenegro

2001-01-01

Full Text Available Se evalu6 el incremento en altura y gro- SOT de rebrotes, la altura del tallo dominante, la alliodoproducci6n de palmito y el numero de rebrotes diapOT cepa en plantaciones sin y bajo 2 poblacio- domines de laurel (92 y 370 arboles/ha y el efecto ofdel sombreamiento, en la zona Atlantica de Costa Rica. Se observ6 un mayor incremento en el diametro y la altura de los rebrotes en el monocultivo (P=0.0403, P=0.0033 respectiva- mente unicamente en los estadios iniciales de crecimiento. Una mayor altura del tallo princi- pal (P=0.0143se observ6 en la asociaci6n con haraltas densidades de arboles de laurel. EI nume- ro de rebrotes pOT cepa fue mayor (P=0.05 en maxilas parcelas sin la presencia de arboles. Las ma- yores producciones de palmito fueron encon- whetradas en el monocultivo y con 92 arboles ha/(P=O.O 117, determinandose un menor ren- dimiento con mayores niveles de intercepci6n de luz fotosinteticamente activa pOT parte del estrato arb6reo (r=0.68, P=0.0415. Los ingre- monosos fueron mayores en el tratamiento sin arbo- les, los cuales ejercieron un efecto negativo so- bre el rendimiento productivo del cultivo.

Patterns of diametric growth in stem-analyzed laurel trees (Cordia alliodora) in a Panamanian forest

Science.gov (United States)

Bernard R Parresol; Margaret S. Devall

2013-01-01

Based on cross-dated increment cores, yearly diameters of trees were reconstructed for 21 laurels (Cordia alliodora) growing in a natural secondary forest on Gigante Peninsula, Panama. From this sample of dominant-codominant trees, ages were 14–35 years with an average of 25 years. Growth typically slowed at 7 years old, indicating effects of...

STS-107 M.S. Laurel Clark during TCDT M113 training activities

Science.gov (United States)

2002-01-01

KENNEDY SPACE CENTER, FLA. -- STS-107 Mission Specialist Laurel Clark (in yellow cap) is instructed on the operation of an M113 armored personnel carrier during Terminal Countdown Demonstration Test activities, a standard part of launch preparations. STS-107 is a mission devoted to research and will include more than 80 experiments that will study Earth and space science, advanced technology development, and astronaut health and safety. Launch is planned for Jan. 16, 2003, between 10 a.m. and 2 p.m. EST aboard Space Shuttle Columbia.

Sources of water to wells in updip areas of the Wenonah-Mount Laurel aquifer, Gloucester and Camden Counties, New Jersey

Science.gov (United States)

Watt, Martha K.; Voronin, Lois M.

2006-01-01

Since 1996, when the New Jersey Department of Environmental Protection (NJDEP) restricted ground-water withdrawals from the Potomac-Raritan-Magothy aquifer system in the southern New Jersey Coastal Plain as a result of excessive drawdown, Coastal Plain communities have been interested in developing alternate sources of water supply for their residents. The use of ground water from areas near the updip parts of the overlying confined aquifers where withdrawals are not restricted is being considered to meet the demand for drinking water. Concerns have arisen, however, regarding the potential effects of increased withdrawals from these areas on ground-water flow to streams and wetlands as well as to the deeper, confined parts of the aquifers. Therefore, the U.S. Geological Survey, in cooperation with the NJDEP, conducted a study to investigate the sources of water to currently inactive wells in the updip part of the Wenonah-Mount Laurel aquifer in Gloucester and Camden Counties, New Jersey. Of particular interest is whether the primary source of the increased withdrawals is likely to be the aquifer outcrop or the downdip, confined part of the aquifer. The outcrop of the Wenonah-Mount Laurel aquifer covers nearly 8 mi2 (square miles), or about 46 percent of Deptford Township's 17.56-mi2 area. The Deptford Township Municipal Utilities Authority owns six currently (2005) inactive wells in the Wenonah-Mount Laurel aquifer at the southeastern boundary of Deptford Township, 1.25 mi (miles) from the outcrop. For the purposes of this study, an existing ground-water-flow model of the New Jersey Coastal Plain aquifers was used to simulate ground-water-flow conditions in Gloucester and Camden Counties in 1998. Two alternative withdrawal scenarios were superimposed on the results of the 1998 simulation. In the first (the 'full-allocation' scenario), full-allocation withdrawal rates established by the NJDEP were applied to 45 existing wells in the Deptford Township area. In the

Susceptibility to Laurel Wilt and disease incidence in two rare plant species, Pondberry and Pondspice Plant Disease.

Science.gov (United States)

Stephen Fraedrich; T Harrington; C Bates; J Johnson; L. Reid; Glenda Susan Best; T Leininger; Tracy Hawkins

2011-01-01

Laurel wilt, caused by Raffaelea lauricola, has been responsible for extensive losses of redbay (Persea borbonia) in South Carolina and Georgia since 2003. Symptoms of the disease have been noted in other species of the Lauraceae such as the federally endangered pondberry (Lindera melissifolia) and the threatened pondspice (Litsea aestivalis). Pondberry and pondspice...

Vertical Distribution and Daily Flight Periodicity of Ambrosia Beetles (Coleoptera: Curculionidae) in Florida Avocado Orchards Affected by Laurel Wilt

Science.gov (United States)

Ambrosia beetles have emerged as significant pests of avocado (Persea americana Miller) due to their association with pathogenic fungal symbionts, most notably Raffaelea lauricola, the causal agent of laurel wilt. We evaluated the interaction of ambrosia beetles with host avocado trees by documentin...

Origin, development, and impact of mountain laurel thickets on the mixed-oak forests of the central Appalachian Mountains, USA

Science.gov (United States)

Patrick H. Brose

2016-01-01

Throughout forests of the northern hemisphere, some species of ericaceous shrubs can form persistent understories that interfere with forest regeneration processes. In the Appalachian Mountains of eastern North America, mountain laurel (Kalmia latifolia) may interfere in the regeneration of mixed-oak (Quercus spp.) forests. To...

Detection and management of Xyleborus glabratus and other vectors of laurel wilt, a lethal disease affecting avocados in Florida

Science.gov (United States)

The redabay ambrosia beetle, Xyleborus glabratus, carries a phytopathogenic symbiont, Raffaelea lauricola, which causes laurel wilt, a lethal vascular disease of some Lauraceae species. Both X. glabratus and R. lauricola are natives of Asia that recently invaded much of the coastal plain of the sout...

First report of Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae) and laurel wilt in Louisiana, USA: The disease continues westward on sassafras

Science.gov (United States)

Stephen W. Fraedrich; C. Wood Johnson; Roger D. Menard; Thomas C. Harrington; Rabiu Olatinwo; G. Susan Best

2015-01-01

Laurel wilt, caused by Raffaelea lauricola Harrington, Fraedrich & Aghayeva (Ophiostomatales: Ophiostomataceae), has spread rapidly through the coastal plains forests of the southeastern United States (USA) with devastating effects on redbay (Persea borbonia [L.] Spreng.; Laurales: Lauraceae) populations (Fraedrich et...

Chapter 11 - Progression of Laurel Wilt Disease in Georgia: 2009–11 (Project SC-EM-08-02)

Science.gov (United States)

R. Scott Cameron; Chip Bates; James Johnson

2014-01-01

Laurel wilt disease (LWD) is caused by the fungus Raffaelea lauricola and vectored by the redbay ambrosia beetle (RAB), Xyleborus glabratus (Fraedrich and others 2008). The pathogen and vector were apparently introduced from Asia through the Port of Savannah, and the disease has spread rapidly throughout the lower coastal plains forests in Georgia, killing nearly all...

Evaluating population and community structure against climate and land-use determinants to improve the conservation of the rare Narcissus pseudonarcissus subsp. nobilis

Directory of Open Access Journals (Sweden)

Vaz, Ana Sofia

2016-06-01

Full Text Available Climate and land-use changes are among the most relevant determinants of future persistence of rare plant species in rural landscapes. We analysed the structure of populations of a rare plant, Narcissus pseudonarcissus subsp. nobilis, and of their respective plant communities against several environmental variables (climate-, topography-, land-use-, and soil-related in order to identify the pressures that may directly or indirectly affect the persistence of the rare species. Overall, local land-use was the primary determinant of traits related to population renewal and community composition. Specifically, traditional farmlands supported higher community diversity and population individuals. Though moderate land-use intensification seemed to benefit plant community diversity, land abandonment could allow the persistence of N. pseudonarcissus subsp. nobilis populations. Also, a relevant influence of regional environment was perceived on species richness as well as on traits related to population condition, highlighting climate change as a potential determinant of the future persistence of the species. This study highlights the importance of considering key population traits as well as of community structure to accomplish conservation goals by accounting with the factors driving changes in the habitats in which rare species occur, from climate change to land-use and landscape management.Las alteraciones del clima y del uso del suelo están entre los factores más relevantes para la persistencia de las especies raras de plantas en paisajes rurales. Este trabajo evalúa la estructura de las poblaciones de una especie rara, Narcissus pseudonarcissus subsp. nobilis, así como de las comunidades de plantas en las cuales se incluyen, frente a variables ambientales (relacionadas con el clima, la topografía, el uso del suelo, y las propiedades del suelo, para identificar las presiones que puedan directa o indirectamente afectar a la especie. En general

Anti-mycobacterial activity of polyketides from Penicillium sp. endophyte isolated from Garcinia nobilis against Mycobacteriumsmegmatis.

Science.gov (United States)

Jouda, Jean Bosco; Mawabo, Isabelle Kamga; Notedji, Augustin; Mbazoa, Céline Djama; Nkenfou, Jean; Wandji, Jean; Nkenfou, Céline Nguefeu

2016-06-01

According to estimates by the World Health Organization, there were 9.6 million new tuberculosis (TB) cases in 2014: 5.4 million among men, 3.2 million among women, and 1.0 million among children. There were also 1.5 million TB deaths. Although there are potent anti-TB molecules, the misuse of these drugs in addition to inconsistent or partial treatment have led to the development of multidrug-resistant TB and extensively drug-resistant TB. It is established that plants harbor microorganisms, collectively known as endophytes, which also produce metabolites. Exploring the as-yet untapped natural products from the endophytes increases the chances of finding novel and active compounds. The present study was aimed to investigate the antimycobacterial activity of the crude extract and compounds isolated from Penicillium sp. endophyte associated with Garcinia nobilis against Mycobacterium smegmatis. Liquid culture obtained from the fermentation of Penicillium sp. was extracted using ethylacetate and the liquid chromatography-mass spectrometry monitored fractionation of crude extracts yielded six compounds. Their structures were elucidated with spectroscopic analyses including two-dimensional nuclear magnetic resonance, high resolution mass spectrometry by dereplication using Antibase, and by comparison to literature data. All compounds and the crude extract from the liquid medium were evaluated for their antimycobacterial activity against M. smegmatis. In this study, the activity of penialidins A-C (1-3), citromycetin (4), p-hydroxy phenyl glyoxalaldoxime (5), and Brefeldin A (6) were tested against nonpathogenic M. smegmatis. Penialidin C was the most active compound with a minimum inhibitory concentration of 15.6μg/mL. Isolated compounds from Penicillium sp. harbored in G. nobilis exhibited promising antimycobacterial activity against M. smegmatis thus supporting the immensity of the potential of antimycobacterial drug discovery from endophytes from medicinal plants

Effect of environmental conditions and lesion age on sporulation of Phytophthora ramorum on California bay laurel, rhododendron, and camellia

Science.gov (United States)

Steve Tjosvold; David Chambers; Sylvia Mori

2013-01-01

The objective of our research was to determine the environmental conditions and lesion age favorable for Phytophthora ramorum sporulation under field conditions. For 2 years, new camellia, rhododendron, and California bay laurel (Umbellaria californica (Hook. & Arn.) Nutt.) nursery stock were seasonally inoculated (every 3 months) on foliage....

Botryosphaeriaceae species associated with dieback and canker disease of bay laurel in northern California with the description of Dothiorella californica sp. nov.

Science.gov (United States)

Lawrence, Daniel P; Peduto Hand, Francesca; Gubler, W Douglas; Trouillas, Florent P

2017-04-01

Members of the Botryosphaeriaceae are cosmopolitan fungi that may exist as seemingly innocuous endophytes or as destructive pathogens of numerous woody hosts, including fruit and nut crops, urban ornamental trees and shrubs, and forest trees. Surveys of bay laurel in northern California have revealed symptoms of dieback and branch canker of unknown aetiology. The goals of this study were to identify and clarify the species of Botryosphaeriaceae associated with these symptoms and to confirm their pathogenicity. To understand the role of members of the Botryosphaeriaceae in the dieback and canker disease of bay laurel, 23 isolates were isolated from symptomatic wood. Phylogenetic analyses of ITS, translation elongation factor 1-α, and beta-tubulin revealed three species: Botryosphaeria dothidea, Neofusicoccum nonquaesitum, and the newly described and typified species Dothiorella californica sp. nov. When select isolates were inoculated to 2- to 3-year-old branches of Umbellularia californica in a natural forest, both B. dothidea and N. nonquaesitum were pathogenic with N. nonquaesitum producing the largest lesions at 12- and 18-months post inoculation, respectively, while Do. californica did not cause wood lesions significantly greater than the mock-inoculated controls. This study represents the first attempt to identify and test the pathogenicity of Botryosphaeriaceae species associated with dieback and canker disease of bay laurel in a northern California forest. Copyright © 2016 British Mycological Society. All rights reserved.

Activity, polypeptide and gene identification of thylakoid Ndh complex in trees: potential physiological relevance of fluorescence assays.

Science.gov (United States)

Serrot, Patricia H; Sabater, Bartolomé; Martín, Mercedes

2012-09-01

Three evergreen (Laurus nobilis, Viburnum tinus and Thuja plicata) and two autumnal abscission deciduous trees (Cydonia oblonga and Prunus domestica) have been investigated for the presence (zymogram and immunodetection) and functionality (post-illumination chlorophyll fluorescence) of the thylakoid Ndh complex. The presence of encoding ndh genes has also been investigated in T. plicata. Western assays allowed tentative identification of zymogram NADH dehydrogenase bands corresponding to the Ndh complex after native electrophoresis of solubilized fractions from L. nobilis, V. tinus, C. oblonga and P. domestica leaves, but not in those of T. plicata. However, Ndh subunits were detected after SDS-PAGE of thylakoid solubilized proteins of T. plicata. The leaves of the five plants showed the post-illumination chlorophyll fluorescence increase dependent on the presence of active Ndh complex. The fluorescence increase was higher in autumn in deciduous, but not in evergreen trees, which suggests that the thylakoid Ndh complex could be involved in autumnal leaf senescence. Two ndhB genes were sequenced from T. plicata that differ at the 350 bp 3' end sequence. Comparison with the mRNA revealed that ndhB genes have a 707-bp type II intron between exons 1 (723 bp) and 2 (729 bp) and that the UCA 259th codon is edited to UUA in mRNA. Phylogenetically, the ndhB genes of T. plicata group close to those of Metasequoia, Cryptomeria, Taxodium, Juniperus and Widdringtonia in the cupresaceae branch and are 5' end shortened by 18 codons with respect to that of angiosperms. Copyright © Physiologia Plantarum 2012.

Presence and Prevalence of Raffaelea lauricola, Cause of Laurel Wilt, in Different Species of Ambrosia Beetle in Florida, USA.

Science.gov (United States)

Ploetz, Randy C; Konkol, Joshua L; Narvaez, Teresa; Duncan, Rita E; Saucedo, Ramon J; Campbell, Alina; Mantilla, Julio; Carrillo, Daniel; Kendra, Paul E

2017-04-01

We summarize the information available on ambrosia beetle species that have been associated in Florida with Raffaelea lauricola T.C. Harr., Fraedrich & Aghayeva, the primary symbiont of Xyleborus glabratus Eichhoff and cause of laurel wilt. In total, 14 species in Ambrosiodmus, Euwallacea, Premnobius, Xyleborus, Xyleborinus, and Xylosandrus were either reared from laurel wilt-affected host trees or trapped in laurel wilt-affected stands of the same, and assayed for R. lauricola. In six collections from native species in the southeastern United States [Persea borbonia (L.), Persea palustris (Raf.) Sarg., and Persea humilis Nash] and four from avocado (Persea americana Mill.), extracted mycangia or heads (taxa with mandibular mycangia) or intact bodies (taxa with mycangia in other locations) were surface-disinfested before assays on a semi-selective medium for the isolation of Raffaelea (CSMA+). Raffaelea lauricola was identified based on its characteristic phenotype on CSMA+, and the identity of a random subset of isolates was confirmed with taxon-specific microsatellite markers. The pathogen was recovered from 34% (246 of 726) of the individuals that were associated with the native Persea spp., but only 6% (58 of 931) of those that were associated with avocado. Over all studies, R. lauricola was recovered from 10 of the ambrosia beetle species, but it was most prevalent in Xyleborus congeners. This is the first record of R. lauricola in Ambrosiodmus lecontei Hopkins, Xyleborinus andrewesi (Blandford), and Xyleborus bispinatus Eichhoff. The potential effects of R. lauricola's promiscuity are discussed. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Tree Species Composition and Structure near Road Borders in the Laurel Forest of Anaga (Tenerife – Islas Canarias

Directory of Open Access Journals (Sweden)

Zaira Negrín

2016-11-01

Full Text Available Urbanization is one of the main causes of species extinction. Closely linked to urbanization are road systems, which are a source of biotic and abiotic effects on the surrounding landscape. The continued existence of these corridors results in enormous human activity (Forman & Alexander, 1998. In particular, roads sharply define and fragment forest ecosystems leading to changes in plant species composition and vegetation structure from road border to the surrounding interior. This paper assesses border effects on tree species richness and composition in the laurel forest of Anaga, Tenerife, Spain. Effects of anthropogenic corridors on vegetation differed among the study sites. Multivariate analysis revealed that species composition is more related to the sampling site than to the effect of the corridor, while for density, significant differences were found between the road border and forest interior but not as a regular pattern. This suggests that main corridor disturbances regarding tree basal area is limited to the immediate road edge in the laurel forest, while for species composition, no significant differences were found.

The identity of the semiterrestrial crab Terrathelphusa kuchingensis (Nobili, 1901) (Crustacea: Decapoda: Brachyura: Gecarcinucidae), with descriptions of four new species from southwestern Sarawak, Borneo, Malaysia.

Science.gov (United States)

Grinang, Jongkar; Ng, Peter K L

2015-04-10

Four new species of semiterrestrial gecarcinucid crabs are described from limestone and sandstone habitats in southwestern Sarawak, Malaysia: Terrathelphusa aglaia n. sp., T. cerina n. sp., T. kundong n. sp., and T. mas n. sp. The taxonomy of T. kuchingensis (Nobili, 1901) is discussed, its precise identity ascertained from fresh material, and its actual distribution determined. This increases the number of Terrathelphusa species in Borneo to eight.

STS-107 M.S. Laurel Clark takes a break during TCDT M113 training

Science.gov (United States)

2002-01-01

KENNEDY SPACE CENTER, FLA. -- STS-107 Mission Specialist Laurel Clark takes a break during training on the operation of an M113 armored personnel carrier during Terminal Countdown Demonstration Test activities, a standard part of launch preparations. STS-107 is a mission devoted to research and will include more than 80 experiments that will study Earth and space science, advanced technology development, and astronaut health and safety. Launch is planned for Jan. 16, 2003, between 10 a.m. and 2 p.m. EST aboard Space Shuttle Columbia.

Field test of foliar-spray herbicides to control mountain laurel in mature mixed-oak forests in western Maryland

Science.gov (United States)

Gary W. Miller; Patrick H. Brose; Jeffrey D. Kochenderfer; James N. Kochenderfer; Kurt W. Gottschalk; John R. Denning

2016-01-01

Successful oak (Quercus spp.) regeneration requires the presence of competitive sources of oak reproduction before parent oaks are harvested. Mountain laurel (Kalmia latifolia) in the understory of many Appalachian forests prevents new oak seedlings from receiving adequate sunlight to survive and grow into competitive size classes. This study examined the efficacy of...

Utility of essential oils for development of host-based lures for Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae), vector of laurel wilt

Science.gov (United States)

Redbay ambrosia beetle, Xyleborus glabratus, is native to Southeast Asia, but subsequent to introduction in Georgia in 2002, it has become a serious invasive pest in the USA, now established in nine southeastern states. Females vector Raffaelea lauricola, the fungus that causes laurel wilt, a letha...

Laurel forest recovery during 20 years in an abandoned firebreak in Tenerife, Canary Islands

Science.gov (United States)

Ramón Arévalo, José; Dolores Peraza, María; Álvarez, Carlos; Bermúdez, Alfredo; Domingo Delgado, Juan; Gallardo, Antonio; María Fernández-Palacios, José

2008-01-01

This study assessed the recovery of the structure and species composition of a laurel forest in an abandoned firebreak in the Rural Park of Anaga, Tenerife (Canary Islands). We statistically compared values of species richness, density and biovolume between 23 plots in the firebreak and six control plots in natural forest near the firebreak. We evaluated changes in species composition with detrended correspondence analysis (DCA) based on densities and biovolume. Biovolume is increasing significantly along the successional gradient (from 1990 to 2004) but remains less than the values in control plots. Stem densities were significantly lower in control plots than in 2004 plots. Species richness was significantly higher in control plots than in 2004 plots (although there were no differences in values obtained between the first sampling period 6 years after abandonment, and the second sampling 20 years after abandonment). Changes in species richness are significant, but all species present in control plots are also found in the firebreak plots. DCA based on biovolume significantly discriminated control plots from firebreak plots in 1990 (for axis I). Results suggest recovery to a laurel forest is occurring, although more time will be required to reach control plot density and biovolume values. The low intensity of disturbance and a well-conserved forest adjacent to the firebreak favour the recovery of species inside the firebreak. We advise eliminating suckers from all small trees (leaving the bigger stems) to accelerate succession to a vegetation structure similar to that found surrounding the firebreak.

Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum.

Science.gov (United States)

Kalai-Grami, Leila; Saidi, Sabrine; Bachkouel, Sarra; Ben Slimene, Imen; Mnari-Hattab, Monia; Hajlaoui, Mohamed Rebah; Limam, Ferid

2014-09-01

A collection of 200 bacterial isolates recovered from citrus plants (Citrus limon, Citrus sinensis, and Citrus reticulata), Medicago truncatula and Laurus nobilis, was established. In vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens Phoma tracheiphila and Verticillium albo-atrum. Isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (PGPB) abilities, as well as for the presence of nonribosomal peptide synthetase (NRPS) genes responsible of the lipopeptide biosynthesis. The results were positive for 16 isolates which exhibited at least two PGPB activities and a single NRPS gene. Genetic diversity of the selected isolates was studied using random amplified polymorphic DNA (RAPD) and repetitive element PCR (REP) tools that showed clustering of strains into three major groups (I, II, and III) (i, ii, and iii), respectively. Clustering was further confirmed by the 16S rDNA sequencing that assigned nine isolates to Bacillus velezensis, four isolates to Bacillus methyltrophicus, one isolate to Bacillus amyloliquefaciens, and two isolates to Bacillus mojavensis. Organ-bacterial genotype interaction as well as positive correlation with NRPS genes are discussed.

HPLC mapping of second generation ethanol production with lignocelluloses wastes and diluted sulfuric hydrolysis

Directory of Open Access Journals (Sweden)

Diogo José Horst

2014-09-01

Full Text Available Wood wastes are potential material for second generation ethanol production within the concept of residual forest bio-refinery. Current paper reports on ethanol production employing an HPLC method for monitoring the chemical content dispersed in the hydrolysate liquor after fermented. The proton-exchange technique was the analytical method employed. Twelve types of wood chips were used as biomass, including Hymenolobium petraeum, Tabebuia cassinoides, Myroxylon peruiferum, Nectandra lanceolata, Ocotea catharinensis, Cedrelinga catenaeformis, Cedrela fissilis Vell, Ocotea porosa, Laurus nobilis, Balfourodendron riedelianum, Pinus Elliotti and Brosimum spp. The influence of diluted sulfuric hydrolysis on the yeast Saccharomyces cerevisiae during the fermentation assay was also investigated. Standard compounds mapped in the analysis comprised fructose, lactic acid, acetic acid, glycerol, glucose and ethanol. The yeast showed ethanol productivity between 0.75 and 1.91 g L-1 h-1, respectively, without the addition of supplementary nutrients or detoxification. The use of these materials for the bioconversion of cellulose into ethanol has been proved. Current analysis contributes towards the production of biofuels by wastes recovery and by process monitoring and optimization.

Laurel Clark Earth Camp: A Program for Teachers and Students to Explore Their World and Study Global Change Through Field-Experience and Satellite Images

Science.gov (United States)

Buxner, S.; Orchard, A.; Colodner, D.; Schwartz, K.; Crown, D. A.; King, B.; Baldridge, A.

2012-03-01

The Laurel Clark Earth Camp program provides middle and high school students and teachers opportunities to explore local environmental issues and global change through field-experiences, inquiry exercises, and exploring satellite images.

Evaluation of the Anti-plasmodial Activity of the Methanolic Root Extracts of Anthocleista nobilis G. Don, Nauclea latifolia Smith and Napoleona imperialis P. Beauv

OpenAIRE

Ijeoma H. Ogbuehi; Omotayo O. Ebong; Eme O. Asuquo; Chijioke A. Nwauche

2014-01-01

The emergence of resistant strains of the malaria parasite has necessitated the continued search for other effective, safe and cheap plant-based anti-malarial agents. This study was carried out to evaluate in vivo the anti-plasmodial effect of the extract of a combination of three plants as used in traditional medicine in South-east, Nigeria. Dried and ground roots of the three plants: Anthocleista nobilis, Nauclea latifolia and Napoleona imperialis were extracted in 70% methanol as a combina...

Fog reduces transpiration in tree species of the Canarian relict heath-laurel cloud forest (Garajonay National Park, Spain).

Science.gov (United States)

Ritter, Axel; Regalado, Carlos M; Aschan, Guido

2009-04-01

The ecophysiologic role of fog in the evergreen heath-laurel 'laurisilva' cloud forests of the Canary Islands has not been unequivocally demonstrated, although it is generally assumed that fog water is important for the survival and the distribution of this relict paleoecosystem of the North Atlantic Macaronesian archipelagos. To determine the role of fog in this ecosystem, we combined direct transpiration measurements of heath-laurel tree species, obtained with Granier's heat dissipation probes, with micrometeorological and artificial fog collection measurements carried out in a 43.7-ha watershed located in the Garajonay National Park (La Gomera, Canary Islands, Spain) over a 10-month period. Median ambient temperature spanned from 7 to 15 degrees C under foggy conditions whereas higher values, ranging from 9 to 21 degrees C, were registered during fog-free periods. Additionally, during the periods when fog water was collected, global solar radiation values were linearly related (r2=0.831) to those under fog-free conditions, such that there was a 75+/-1% reduction in median radiation in response to fog. Fog events greatly reduced median diurnal tree transpiration, with rates about 30 times lower than that during fog-free conditions and approximating the nighttime rates in both species studied (the needle-like leaf Erica arborea L. and the broadleaf Myrica faya Ait.). This large decrease in transpiration in response to fog was independent of the time of the day, tree size and species and micrometeorological status, both when expressed on a median basis and in cumulative terms for the entire 10-month measuring period. We conclude that, in contrast to the turbulent deposition of fog water droplets on the heath-laurel species, which may be regarded as a localized hydrological phenomenon that is important for high-altitude wind-exposed E. arborea trees, the cooler, wetter and shaded microenvironment provided by the cloud immersion belt represents a large-scale effect

Entomopathogenic fungi as a biological control agents for the vector of the laurel wilt disease, the redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae)

Science.gov (United States)

The redbay ambrosia beetle (RAB), Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae) vectors the fungal pathogen, Raffaelea lauricola, which causes laurel wilt (LW), a lethal disease of trees in the family Lauraceae, including the most commercially important crop in this family, avocado, Pe...

Isolations from the redbay ambrosia beetle, Xyleborus glabratus, confirm that the laurel wilt pathogen, Raffaelea lauricola, originated in Asia

Science.gov (United States)

Thomas C. Harrington; Hye Young Yun; Sheng-Shan Lu; Hideaki Goto; Dilzara N. Aghayeva; Stephen W. Fraedrich

2011-01-01

The laurel wilt pathogen Raffaelea lauricola was hypothesized to have been introduced to the southeastern USA in the mycangium of the redbay ambrosia beetle, Xyleborus glabratus, which is native to Asia. To test this hypothesis adult X. glabratus were trapped in Taiwan and on Kyushu Island, Japan, in 2009, and dead beetles were sent to USA for isolation of fungal...

Suitability of California bay laurel and other species as hosts for the non-native redbay ambrosia beetle and granulate ambrosia beetle.

Science.gov (United States)

Albert (Bud) Mayfield; Martin MacKenzie; Philip G. Cannon; Steve Oak; Scott Horn; Jaesoon Hwang; Paul E. Kendra

2013-01-01

The redbay ambrosia beetle Xyleborus glabratus Eichhoff is a non-native vector of the pathogen that causes laurel wilt, a deadly disease of trees in the family Lauraceae in the southeastern U.S.A.Concern exists that X. glabratus and its fungal symbiont could be transported to the western U....

DFS-88, 1988 Tri-Service Data Fusion Symposium. Volume I - Technical Proceedings, Johns Hopkins University, Applied Physics Laboratory, Laurel, Maryland, 17 - 19 May 1988

National Research Council Canada - National Science Library

1988-01-01

The 1988 Tri-Service Data Fusion Symposium (DFS-88) was held at Laurel, Maryland on 17-19 May 1988 under the joint sponsorship of the Data Fusion Sub-Panel of the Joint Directors of Laboratories (JDL/DFSP...

Survey of insect fauna from plants medicinal, aromatic and seasoning and disinfestation by the process of radiation

International Nuclear Information System (INIS)

Reis, Fabricio Caldeira

2013-01-01

The present study aimed to survey the insect fauna associated with medicinal plants, aromatic dehydrated and seasoning trade in Sao Paulo city, using different doses of gamma radiation with the aim of disinfestation of the material and determine the lethal dose of gamma radiation on Sphaericus gibboides. From April to May 2011 were collected in 10 establishments the following sample materials: Melissa officinalis L. (Lemongrass), Mentha piperita L. (Mint), Ocimum basilicum L. (Basil), Origanum vulgare L. (Oregano), Rosmarinus officinalis L. (Rosemary), Thymus vulgaris L. (Thyme), Senna alexandrina Mill (senna), Coriandrum sativum L. (Coriander), Petroselinum crispum (Mill.) Fuss (salsa) and Pimpinella anisum L. (Fennel), Baccharis trimera (Less.) DC. (Gorse), Chamomilla recutita L. (= M. recutita L.) (chamomile), Laurus nobilis L. (Blonde) (Lauraceae); Capsicum annuum L. (Sweet paprika), Bixa orellana L. (Spice) (Bixaceae) and Peumus boldus Molina (Boldo). The first screening showed that all the tested materials did not show the presence of adult insects. After 45 days 940 adult insects were found and larvae from eggs. The substrates analyzed Chamomilla recutita showed the highest rate of infestation, with 70,6%. Pelmus boldus, Laurus nobilis, Chamomilla recutita and Capsicum annuum, had the highest species diversity. Baccharis trimera, Bixa orellana, Melissa officinalis, Origanum vulgare and Coriandrum sativum showed no infestation. The species was Lasioderma serricorne the insect with the largest number of individuals found (936), higher percentage of infestation in different materials (62.5%) and lots, and highest occurrence (68,75%) materials (M. piperita, S. alexandrian, P. anisum, Chamomilla recutita, P. crispum, L. nobilis, C. sativum, C. annuum, O. basilicum, P. boldus and T. vulgaris). The following materials were selected for testing disinfestation by irradiation process: Bixa orellana, Capsicum annuum, Cassia angustifolia, Coriandrum sativum, Mentha

Survey of insect fauna from plants medicinal, aromatic and seasoning and disinfestation by the process of radiation; Levantamento da entomofauna de plantas medicinais, aromaticas e condimentares e desinfestacao pelo processo de irradiacao

Energy Technology Data Exchange (ETDEWEB)

Reis, Fabricio Caldeira

2013-07-01

The present study aimed to survey the insect fauna associated with medicinal plants, aromatic dehydrated and seasoning trade in Sao Paulo city, using different doses of gamma radiation with the aim of disinfestation of the material and determine the lethal dose of gamma radiation on Sphaericus gibboides. From April to May 2011 were collected in 10 establishments the following sample materials: Melissa officinalis L. (Lemongrass), Mentha piperita L. (Mint), Ocimum basilicum L. (Basil), Origanum vulgare L. (Oregano), Rosmarinus officinalis L. (Rosemary), Thymus vulgaris L. (Thyme), Senna alexandrina Mill (senna), Coriandrum sativum L. (Coriander), Petroselinum crispum (Mill.) Fuss (salsa) and Pimpinella anisum L. (Fennel), Baccharis trimera (Less.) DC. (Gorse), Chamomilla recutita L. (= M. recutita L.) (chamomile), Laurus nobilis L. (Blonde) (Lauraceae); Capsicum annuum L. (Sweet paprika), Bixa orellana L. (Spice) (Bixaceae) and Peumus boldus Molina (Boldo). The first screening showed that all the tested materials did not show the presence of adult insects. After 45 days 940 adult insects were found and larvae from eggs. The substrates analyzed Chamomilla recutita showed the highest rate of infestation, with 70,6%. Pelmus boldus, Laurus nobilis, Chamomilla recutita and Capsicum annuum, had the highest species diversity. Baccharis trimera, Bixa orellana, Melissa officinalis, Origanum vulgare and Coriandrum sativum showed no infestation. The species was Lasioderma serricorne the insect with the largest number of individuals found (936), higher percentage of infestation in different materials (62.5%) and lots, and highest occurrence (68,75%) materials (M. piperita, S. alexandrian, P. anisum, Chamomilla recutita, P. crispum, L. nobilis, C. sativum, C. annuum, O. basilicum, P. boldus and T. vulgaris). The following materials were selected for testing disinfestation by irradiation process: Bixa orellana, Capsicum annuum, Cassia angustifolia, Coriandrum sativum, Mentha

Quantification of propagules of the laurel wilt fungus and other mycangial fungi from the redbay ambrosia beetle, Xyleborus glabratus.

Science.gov (United States)

Harrington, T C; Fraedrich, S W

2010-10-01

The laurel wilt pathogen, Raffaelea lauricola, is a fungal symbiont of the redbay ambrosia beetle, Xyleborus glabratus, which is native to Asia and was believed to have brought R. lauricola with it to the southeastern United States. Individual X. glabratus beetles from six populations in South Carolina and Georgia were individually macerated in glass tissue grinders and serially diluted to quantify the CFU of fungal symbionts. Six species of Raffaelea were isolated, with up to four species from an individual adult beetle. The Raffaelea spp. were apparently within the protected, paired, mandibular mycangia because they were as numerous in heads as in whole beetles, and surface-sterilized heads or whole bodies yielded as many or more CFU as did nonsterilized heads or whole beetles. R. lauricola was isolated from 40 of the 41 beetles sampled, and it was isolated in the highest numbers, up to 30,000 CFU/beetle. Depending on the population sampled, R. subalba or R. ellipticospora was the next most frequently isolated species. R. arxii, R. fusca, and R. subfusca were only occasionally isolated. The laurel wilt pathogen apparently grows in a yeast phase within the mycangia in competition with other Raffaelea spp.

Transport and characterization of ambient biological aerosol near Laurel, MD

Science.gov (United States)

Santarpia, J. L.; Cunningham, D.; Gilberry, J.; Kim, S.; Smith, E. E.; Ratnesar-Shumate, S.; Quizon, J.

2010-09-01

Bacterial aerosol have been observed and studied in the ambient environment since the mid nineteenth century. These studies have sought to provide a better understanding of the diversity, variability and factors that control the biological aerosol population. In this study, we show comparisons between diversity of culturable bacteria and fungi, using culture and clinical biochemical tests, and 16S rRNA diversity using Affymetrix PhyloChips. Comparing the culturable fraction and surveying the total 16S rRNA of each sample provides a comprehensive look at the bacterial population studied and allows comparison with previous studies. Thirty-six hour back-trajectories of the air parcels sampled, over the two day period beginning 4 November 2008, provide information on the sources of aerosol sampled on the campus of Johns Hopkins University Applied Physics Laboratory in Laurel, MD. This study indicates that back-trajectory modeling of air parcels may provide insights into the observed diversity of biological aerosol.

Epicuticular wax on cherry laurel (Prunus laurocerasus) leaves does not constitute the cuticular transpiration barrier.

Science.gov (United States)

Zeisler, Viktoria; Schreiber, Lukas

2016-01-01

Epicuticular wax of cherry laurel does not contribute to the formation of the cuticular transpiration barrier, which must be established by intracuticular wax. Barrier properties of cuticles are established by cuticular wax deposited on the outer surface of the cuticle (epicuticular wax) and in the cutin polymer (intracuticular wax). It is still an open question to what extent epi- and/or intracuticular waxes contribute to the formation of the transpiration barrier. Epicuticular wax was mechanically removed from the surfaces of isolated cuticles and intact leaf disks of cherry laurel (Prunus laurocerasus L.) by stripping with different polymers (collodion, cellulose acetate and gum arabic). Scanning electron microscopy showed that two consecutive treatments with all three polymers were sufficient to completely remove epicuticular wax since wax platelets disappeared and cuticle surfaces appeared smooth. Waxes in consecutive polymer strips and wax remaining in the cuticle after treatment with the polymers were determined by gas chromatography. This confirmed that two treatments of the polymers were sufficient for selectively removing epicuticular wax. Water permeability of isolated cuticles and cuticles covering intact leaf disks was measured using (3)H-labelled water before and after selectively removing epicuticular wax. Cellulose acetate and its solvent acetone led to a significant increase of cuticular permeability, indicating that the organic solvent acetone affected the cuticular transpiration barrier. However, permeability did not change after two subsequent treatments with collodion and gum arabic or after treatment with the corresponding solvents (diethyl ether:ethanol or water). Thus, in the case of P. laurocerasus the epicuticular wax does not significantly contribute to the formation of the cuticular transpiration barrier, which evidently must be established by the intracuticular wax.

Antibacterial activities of medicinal plants against multidrug resistant urinary tract pathogens

International Nuclear Information System (INIS)

Aziz, M.A.; Adnan, M.; Rahman, H.; Allah, A.; Hashem, A.

2017-01-01

Urinary tract infections (UTI) caused by multi-drug resistant (MDR) bacterial pathogens have become a serious global health concern. Main etiological agents for UTI are Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa. Recently, medicinal plants have found great popularity in medical treatment for different kinds of infections including urinary tract infections. The study has been planned to evaluate the efficacy of alkaloids, flavonoids, saponins and crude extracts of medicinal plants i.e. Syzygium aromaticum, Glycerrhiza glabra,Laurus nobilis and Brassica rapa against MDR urinary tract pathogens through agar well diffusion method. To investigate the Minimum Inhibitory Concentrations (MICs) and Minimum Bactericidal Concentration (MBCs), dilution method was used. Quantitative evaluations of phytochemicals indicated the presence of alkaloids in higher concentrations. Results obtained for the antibacterial activities, the crude extracts of the four plants showed significantly higher inhibition zones as compared to other phytochemicals. The MIC values obtained for different extracts varying from 7.5-15 mg/ml. Comparig the activities of the extracts of the the four medicinal plants it was found that Syzygium aromaticum was the most potent plant against the tested bacterial pathogens indicating its strong candidateship for the drug development. (author)

Repelência e atividade inseticida de pós vegetais sobre Zabrotes subfasciatus Boheman em feijão-fava armazenado

Directory of Open Access Journals (Sweden)

J.E. Girão Filho

2014-09-01

Full Text Available O objetivo desta pesquisa foi avaliar o potencial inseticida de Piper nigrum L. (pimenta do reino, Ruta graveolens L. (arruda, Laurus nobilis L. (louro, Syzygium aromaticum (L. Merr. & L.M.Perry (cravo da índia, Chenopodium ambrosioides L. (mastruz, Piper tuberculatum Jaqc. (pimenta de macaco, Tagetes erecta L. (cravo de defunto, Cymbopogon nardus L. (citronela e Melissa officinalis L. (erva cidreira sobre Z. subfasciatus Boh. através dos testes de confinamento, onde os insetos foram confinados durante cinco dias em um recipiente contendo dez grãos de feijão-fava com 0,3 g do pó da planta a ser testada, e, com chance de escolha por meio de uma arena circular, os insetos tiveram a possibilidade de escolha entre grãos com os pós e a testemunha, grãos sem pó das plantas. Observou-se que houve plantas que atuaram como inseticida, outras que repeliram o inseto e não causaram a morte, e outras, que além de repelir também mataram os insetos quando em contato (cravo da índia e matruz. Concluímos que: P. nigrum, P. tuberculatum, S. aromaticum e C. ambrosioides são tóxicas à Z. subfasciatus causando-lhes a morte, L. nobilis, T. erecta, e C. nardus não apresentaram efeito tóxico sobre Z. subfasciatus, C. ambrosioides, S. aromaticum, e C. nardus são repelentes à Z. subfasciatus; P. nigrum, P. tuberculatum, e T. erecta são neutras, e que M. officinalis não apresentou nenhum efeito sobre Z. subfasciatus em relação aos parâmetros avaliados.

Density, size structure, shell orientation and epibiontic colonization of the fan mussel Pinna nobilis L. 1758 (Mollusca: Bivalvia in three contrasting habitats in an estuarine area of Sardinia (W Mediterranean

Directory of Open Access Journals (Sweden)

Piero Addis

2009-03-01

Full Text Available We investigated the spatial distribution, size structure, shell orientation and valve colonization by epibionts of the endangered Mediterranean bivalve Pinna nobilis in three continuous but different habitats in the Gulf of Oristano (Sardinia, western Mediterranean. The sampling stations chosen were: an estuarine area (E of coastal salt-marshes characterized by unvegetated sea-bottoms; and two areas in a seagrass meadow characterized by an extensive Posidonia oceanica meadow (Mw and patched mixed meadows of P. oceanica and Cymodocea nodosa (Me. We found significant differences in mean densities among stations and the highest value was found in the estuarine area. Shell orientation showed that there was uniform circular distribution of specimens in the Mw station and a unimodal distribution in the Me and E stations, where specimens were set at 0°N and 10°NNE, which is a pattern related to sea drift. Shell epibiosis displayed differences between habitats. The highest valve colonization was in the estuary, with filamentous dark algae and Ostrea edulis reaching almost 90 percent of shell coverage. This study provides new information on habitat preferences and data for assessing local populations of P. nobilis that is useful for its conservation and improving the knowledge of its ecology.

This tree is not big enough for the both of us: symptoms of Phytophthora ramorum on California bay laurel are lower when insect herbivores are abundant

Science.gov (United States)

Kerry E. Wininger; Nathan Rank

2017-01-01

Leaves of California bay laurel (Umbellularia californica) are considered the primary natural source of inoculum for the devastating forest disease sudden oak death (Phytophthora ramorum), and yet this plant and the insects associated with its leaves remain understudied. This is unfortunate due to the role herbivorous...

Spore Acquisition and Survival of Ambrosia Beetles Associated with the Laurel Wilt Pathogen in Avocados after Exposure to Entomopathogenic Fungi.

Science.gov (United States)

Avery, Pasco B; Bojorque, Verónica; Gámez, Cecilia; Duncan, Rita E; Carrillo, Daniel; Cave, Ronald D

2018-04-25

Laurel wilt is a disease threatening the avocado industry in Florida. The causative agent of the disease is a fungus vectored by ambrosia beetles that bore into the trees. Until recently, management strategies for the vectors of the laurel wilt fungus relied solely on chemical control and sanitation practices. Beneficial entomopathogenic fungi (EPF) are the most common and prevalent natural enemies of pathogen vectors. Laboratory experiments demonstrated that commercial strains of EPF can increase the mortality of the primary vector, Xyleborus glabratus , and potential alternative vectors, Xylosandrus crassiusculus , Xyleborus volvulus and Xyleborus bispinatus (Coleoptera: Curculionidae: Scolytinae). Our study provides baseline data for three formulated commercially-available entomopathogenic fungi used as potential biocontrol agents against X. crassiusculus , X. volvulus and X. bispinatus. The specific objectives were to determine: (1) the mean number of viable spores acquired per beetle species adult after being exposed to formulated fungal products containing different strains of EPF ( Isaria fumosorosea , Metarhizium brunneum and Beauveria bassiana ); and (2) the median and mean survival times using paper disk bioassays. Prior to being used in experiments, all fungal suspensions were adjusted to 2.4 × 10⁶ viable spores/mL. The number of spores acquired by X. crassiusculus was significantly higher after exposure to B. bassiana , compared to the other fungal treatments. For X. volvulus , the numbers of spores acquired per beetle were significantly different amongst the different fungal treatments, and the sequence of spore acquisition rates on X. volvulus from highest to lowest was I. fumosorosea > M. brunneum > B. bassiana . After X. bispinatus beetles were exposed to the different suspensions, the rates of acquisition of spores per beetle amongst the different fungal treatments were similar. Survival estimates (data pooled across two tests) indicated an

Resistance of juveniles of the Mediterranean pen shell, (Pinna nobilis) to hypoxia and interaction with warming

KAUST Repository

Basso, Lorena

2015-05-01

Low oxygen zones in coastal and open ecosystems have expanded in recent decades, a trend that will accelerate with climatic warming. Exposure of nearshore animals to hypoxic waters and increasing temperature may be stressful and lead to impaired physiological performance. Here, we evaluate the effect of low oxygen concentrations in interaction with increased temperature on juveniles of a large Mediterranean bivalve, Pinna nobilis. We examine the response of survival and oxygen consumption of juveniles placed in water with normal and low oxygen concentrations (100% and 40%, respectively) along an increased temperature gradient (24, 26, 28 and 30°C) during 4 days. To our knowledge, this is the first study on hypoxia and its combined effect with temperature on juveniles of this species. We show that the two stressors alone or in combination do not influence the physiological performance of juveniles for short periods (4 days). This result stands apart from a general trend pointing at ocean warming as additional stressor that could increase the vulnerability of benthic macrofauna to reduced oxygen concentrations. © 2015 Elsevier Ltd.

Resistance of juveniles of the Mediterranean pen shell, (Pinna nobilis) to hypoxia and interaction with warming

KAUST Repository

Basso, Lorena; Hendriks, Iris E.; Duarte, Carlos M.

2015-01-01

Low oxygen zones in coastal and open ecosystems have expanded in recent decades, a trend that will accelerate with climatic warming. Exposure of nearshore animals to hypoxic waters and increasing temperature may be stressful and lead to impaired physiological performance. Here, we evaluate the effect of low oxygen concentrations in interaction with increased temperature on juveniles of a large Mediterranean bivalve, Pinna nobilis. We examine the response of survival and oxygen consumption of juveniles placed in water with normal and low oxygen concentrations (100% and 40%, respectively) along an increased temperature gradient (24, 26, 28 and 30°C) during 4 days. To our knowledge, this is the first study on hypoxia and its combined effect with temperature on juveniles of this species. We show that the two stressors alone or in combination do not influence the physiological performance of juveniles for short periods (4 days). This result stands apart from a general trend pointing at ocean warming as additional stressor that could increase the vulnerability of benthic macrofauna to reduced oxygen concentrations. © 2015 Elsevier Ltd.

Water quality and quantity and simulated surface-water and groundwater flow in the Laurel Hill Creek Basin, southwestern Pennsylvania, 1991–2007

Science.gov (United States)

Galeone, Daniel G.; Risser, Dennis W.; Eicholtz, Lee W.; Hoffman, Scott A.

2017-07-10

Laurel Hill Creek is considered one of the most pristine waterways in southwestern Pennsylvania and has high recreational value as a high-quality cold-water fishery; however, the upper parts of the basin have documented water-quality impairments. Groundwater and surface water are withdrawn for public water supply and the basin has been identified as a Critical Water Planning Area (CWPA) under the State Water Plan. The U.S. Geological Survey, in cooperation with the Somerset County Conservation District, collected data and developed modeling tools to support the assessment of water-quality and water-quantity issues for a basin designated as a CWPA. Streams, springs, and groundwater wells were sampled for water quality in 2007. Streamflows were measured concurrent with water-quality sampling at main-stem sites on Laurel Hill Creek and tributaries in 2007. Stream temperatures were monitored continuously at five main-stem sites from 2007 to 2010. Water usage in the basin was summarized for 2003 and 2009 and a Water-Analysis Screening Tool (WAST) developed for the Pennsylvania State Water Plan was implemented to determine whether the water use in the basin exceeded the “safe yield” or “the amount of water that can be withdrawn from a water resource over a period of time without impairing the long-term utility of a water resource.” A groundwater and surface-water flow (GSFLOW) model was developed for Laurel Hill Creek and calibrated to the measured daily streamflow from 1991 to 2007 for the streamflow-gaging station near the outlet of the basin at Ursina, Pa. The CWPA designation requires an assessment of current and future water use. The calibrated GSFLOW model can be used to assess the hydrologic effects of future changes in water use and land use in the basin.Analyses of samples collected for surface-water quality during base-flow conditions indicate that the highest nutrient concentrations in the main stem of Laurel Hill Creek were at sites in the

Impact of Laurel Wilt, Caused by Raffaelea lauricola, on Leaf Gas Exchange and Xylem Sap Flow in Avocado, Persea americana.

Science.gov (United States)

Ploetz, Randy C; Schaffer, Bruce; Vargas, Ana I; Konkol, Joshua L; Salvatierra, Juanpablo; Wideman, Ronney

2015-04-01

Laurel wilt, caused by Raffaelea lauricola, is a destructive disease of avocado (Persea americana). The susceptibility of different cultivars and races was examined previously but more information is needed on how this host responds to the disease. In the present study, net CO2 assimilation (A), stomatal conductance of H2O (gs), transpiration (E), water use efficiency (WUE), and xylem sap flow rates were assessed in cultivars that differed in susceptibility. After artificial inoculation with R. lauricola, there was a close relationship between symptom development and reductions in A, gs, E, WUE, and mean daily sap flow in the most susceptible cultivar, 'Russell', and significantly greater disease and lower A, gs, E, WUE, and sap flow rates were usually detected after 15 days compared with the more tolerant 'Brogdon' and 'Marcus Pumpkin'. Significant differences in preinoculation A, gs, E, and WUE were generally not detected among the cultivars but preinoculation sap flow rates were greater in Russell than in Brogdon and Marcus Pumpkin. Preinoculation sap flow rates and symptom severity for individual trees were correlated at the end of an experiment (r=0.46), indicating that a plant's susceptibility to laurel wilt was related to its ability to conduct water. The potential management of this disease with clonal rootstocks that reduce sap flow rates is discussed.

GIS-based rapid-assessment of bighead carp Hypophthalmichthys nobilis (Richardson, 1845) suitability in reservoirs

Science.gov (United States)

Long, James M.; Liang, Yu; Shoup, Daniel E.; Dzialowski, Andrew R.; Bidwell, Joseph R.

2014-01-01

Broad-scale niche models are good for examining the potential for invasive species occurrences, but can fall short in providing managers with site-specific locations for monitoring. Using Oklahoma as an example, where invasive bighead carp (Hypophthalmichthys nobilis) are established in certain reservoirs, but predicted to be widely distributed based on broad-scale niche models, we cast bighead carp reproductive ecology in a site-specific geospatial framework to determine their potential establishment in additional reservoirs. Because bighead carp require large, long free-flowing rivers with suitable hydrology for reproduction but can persist in reservoirs, we considered reservoir tributaries with mean annual daily discharge ≥8.5 cubic meters per second (m3 /s) and quantified the length of their unimpeded portions. In contrast to published broad-scale niche models that identified nearly the entire state as susceptible to invasion, our site-specific models showed that few reservoirs in Oklahoma (N = 9) were suitable for bighead carp establishment. Moreover, this method was rapid and identified sites that could be prioritized for increased study or scrutiny. Our results highlight the importance of considering the environmental characteristics of individual sites, which is often the level at which management efforts are implemented when assessing susceptibility to invasion.

Acoustic satellite behaviour in the Australian bushcricket Elephantodeta nobilis (Phaneropterinae, Tettigoniidae, Orthoptera).

Science.gov (United States)

Bailey; Field

2000-02-01

Male and female Elephantodeta nobilis duet with the female responding to the male's long and complex call. The duetting male's call consisted of four parts, described here as parts A, B, C and D. We found that the female replied 570 ms after the male's D pulse, which followed the extended part B and short click of part C. Noncalling males were attracted to the duet and often used satellite tactics by inserting a volley of clicks 200 ms before the alpha male's D pulse. Satellite males used part C of the alpha male song to cue their own call and this inserted call induced females to reply earlier compared with the alpha male call alone. Alpha males often extended their calls with additional D-type calls and so we examined the effectiveness of these calls as countermeasures to satellite calling. There was no influence of this alpha strategy on the satellite's propensity to call although more calls from the alpha male did cause the female to reply more frequently. We also examined the effect of relative intensity of alpha and satellite calls on the female's reply. Reduced satellite intensity increased the variance in the timing of the female response. Finally, we tested the effectiveness of the satellite's call on female phonotaxis within a two-speaker arena. Although females preferred the alpha male they were nevertheless attracted to the satellite calls regardless of the latter's relative intensity. We discuss the possible role of satellite calling as a novel conditional strategy. Copyright 2000 The Association for the Study of Animal Behaviour.

Spore Acquisition and Survival of Ambrosia Beetles Associated with the Laurel Wilt Pathogen in Avocados after Exposure to Entomopathogenic Fungi

Directory of Open Access Journals (Sweden)

Pasco B. Avery

2018-04-01

Full Text Available Laurel wilt is a disease threatening the avocado industry in Florida. The causative agent of the disease is a fungus vectored by ambrosia beetles that bore into the trees. Until recently, management strategies for the vectors of the laurel wilt fungus relied solely on chemical control and sanitation practices. Beneficial entomopathogenic fungi (EPF are the most common and prevalent natural enemies of pathogen vectors. Laboratory experiments demonstrated that commercial strains of EPF can increase the mortality of the primary vector, Xyleborus glabratus, and potential alternative vectors, Xylosandrus crassiusculus, Xyleborus volvulus and Xyleborus bispinatus (Coleoptera: Curculionidae: Scolytinae. Our study provides baseline data for three formulated commercially-available entomopathogenic fungi used as potential biocontrol agents against X. crassiusculus, X. volvulus and X. bispinatus. The specific objectives were to determine: (1 the mean number of viable spores acquired per beetle species adult after being exposed to formulated fungal products containing different strains of EPF (Isaria fumosorosea, Metarhizium brunneum and Beauveria bassiana; and (2 the median and mean survival times using paper disk bioassays. Prior to being used in experiments, all fungal suspensions were adjusted to 2.4 × 106 viable spores/mL. The number of spores acquired by X. crassiusculus was significantly higher after exposure to B. bassiana, compared to the other fungal treatments. For X. volvulus, the numbers of spores acquired per beetle were significantly different amongst the different fungal treatments, and the sequence of spore acquisition rates on X. volvulus from highest to lowest was I. fumosorosea > M. brunneum > B. bassiana. After X. bispinatus beetles were exposed to the different suspensions, the rates of acquisition of spores per beetle amongst the different fungal treatments were similar. Survival estimates (data pooled across two tests indicated an

Review of Feminist Bioethics At the Center, On the Margins, edited by Jackie Leach Scully, Laurel E. Baldwin-Ragaven, Petya Fitzpatrick

Directory of Open Access Journals (Sweden)

Sander-Staudt Maureen

2010-12-01

Full Text Available Abstract The anthology, Feminist Bioethics, edited by Jackie Leach Scully, Laurel E. Baldwin-Ragaven, and Petya Fitzpatrick, examines how feminist bioethics theoretically and methodologically challenges mainstream bioethics, and whether these approaches are useful for exploring difference in other contexts. It offers critical conceptual analyses of "autonomy", "universality", and "trust", and covers topics such as testing for hereditary cancer, prenatal selection for sexual orientation, midwifery, public health, disability, Indigenous research reform in Australia, and China's one child policy.

Composition of volatile aromatic compounds and minerals of tarhana enriched with cherry laurel (Laurocerasus officinalis).

Science.gov (United States)

Temiz, Hasan; Tarakçı, Zekai

2017-03-01

Different concentrations of cherry laurel pulp (0, 5, 10, 15 and 20%) were used to produce tarhana samples. Volatile aromatic compounds and minor mineral content were investigated. Volatile aromatic compounds were analyzed by using GC-MS with SPME fiber and minor mineral values were evaluated with inductively coupled plasma optical emission spectrometer. The statistical analysis showed that addition of pulp affected volatile aromatic compounds and minor mineral content significantly. Thirty five volatile aromatic compounds were found in tarhana samples. The octanoic acid from acids, benzaldehyde (CAS) phenylmethanal from aldehydes, 6-methyl-5-hepten-2-one from ketones, octadecane (CAS) n -octadecane form terpenes, ethyl caprylate from esters and benzenemethanol (CAS) benzyl alcohol from alcohols had the highest percentage of volatile aromatic compounds. Tarhana samples were rich source of Mn, Cu and Fe content.

Abundance and reproductive biology of metapenaeus stebbingi nobili, shrimp in Lake Qarum, Egypt

Energy Technology Data Exchange (ETDEWEB)

Abdel Razek, F.A. [National Institute of Oceanography and Fisheries, Kayed Bay , Alexandria, (Egypt)

1995-12-31

After 10 years of shrimp transplantation Lake Qarum, shrimp production became the second product (36.44%), after Tilapia zilli (48%), of the total landing of Lake Qarum, Solea vulgaris production remained stable (7%), but mullets a sharp decrease in its production (3%) during the period from 1985 to 1990. The annual landing of the transplanted Metapenaeus stebbingi Nobili increased from 1.7% in 1978-1979 to 100% in 1900 of the total shrimp landing of Lake Qarum. The success of Metapenaeus stebbingi in Lake Qarum is a direct result of the suitable ecological conditions. The estimated minimum biological size of Metapenaeus stebbingi in the lake was 8.3 cm in total length while the smallest female observed was 9.0 cm long. The highest spawning activity of Metapenaeus stebbingi was from June to October with high abunance of recuritments during August-October. The maximum size recorded was 12.0 cm for females and 11.0 cm for males with a modal size of 4.0-5.0 cm for both sexes. Smaller sizes (less than 4.0 cm) show an abundance of males. The fishing season in lake Qarum begins from July to December and from February to April. The active period of spawning as well as the occurrence of Metapenaeus stebbingi fry is from August to October. This period coincides with the highest fishing operations in the lake. This affects directly the shrimp fisheries of Lake Qarum.

Reexamining the frequency range of hearing in silver (Hypophthalmichthys molitrix) and bighead (H. nobilis) carp

Science.gov (United States)

Vetter, Brooke J.; Brey, Marybeth; Meninger, Allen F.

2018-01-01

Silver (Hypophthalmichthys molitrix) and bighead (H. nobilis) carp (collectively bigheaded carp) are invasive fish that threaten aquatic ecosystems in the upper Midwest United States and the Laurentian Great Lakes. Controlling bigheaded carp is a priority of fisheries managers and one area of focus involves developing acoustic deterrents to prevent upstream migration. For an acoustic deterrent to be effective however, the hearing ability of bigheaded carp must be characterized. A previous study showed that bigheaded carp detected sound up to 3 kHz but this range is narrower than what has been reported for other ostariophysans. Therefore, silver and bighead carp frequency detection was evaluated in response to 100 Hz to 9 kHz using auditory evoked potentials (AEPs). AEPs were recorded from 100 Hz to 5 kHz. The lowest thresholds were at 500 Hz for both species (silver carp threshold: 80.6 ± 3.29 dB re 1 μPa SPLrms, bighead carp threshold: 90.5 ± 5.75 dB re 1 μPa SPLrms; mean ± SD). These results provide fisheries managers with better insight on effective acoustic stimuli for deterrent systems, however, to fully determine bigheaded carp hearing abilities, these results need to be compared with behavioral assessments.

PERTUMBUHAN MATA TUNAS JERUK KEPROK (CITRUS NOBILIS HASIL OKULASI PADA BERBAGAI MEDIA TANAM DAN UMUR BATANG BAWAH ROUGH LEMON (C. JAMBHIRI

Directory of Open Access Journals (Sweden)

Tatiek Kartika Suharsi

2013-08-01

Full Text Available The objective of this study was to evaluate keprok (Citrus nobilis budding seed development on various growing media and age of C. jambhiri rootstock. The design of experiment was complete randomized groups with 2 factors and 3 replications. The growing media consisted of soil, charcoal husk, sheep’s manure, compost, and vermi-compost. Rough lemon citrus rootstock’s age were 8, 11, and 14 months. The results showed that the growing media of soil:charcoal husk:compost or vermicompost mixture of [1:1:1] (v:v:v was the best media for increasing keprok bud growing. Rough lemon rootstock of 8 and 11 months age were the best rootstock for keprok. Rough lemon rootstock of 14 month age stimulated growth of keprok bud faster than that of 8 and 11 month age of rootstock.

ON THE STRUCTURE OF THE MEDIAN SEGMENT OF THE DIGESTIVE TUBE AND ON THE ACTIVITY OF ALPHA-AMYLASE AT ARISTICHTHYS NOBILIS, IN VARIOUS STAGES OF DEVELOPMENT

Directory of Open Access Journals (Sweden)

Gabriela Vasile

2007-12-01

Full Text Available The investigations have been dedicated to the structure of the median segment of the digestive tube, on Aristichthys nobilis individuals of various ages (i.e., one summer- and, respectively, four summer-old, correlated, on one side, with the main feeding regime and, on the other, with the amylasic activity present in this part of the digestive tract. As to the structural aspects, higher values of the thickness of the mucous tunic have been recorded in the four summer-old bighead carp individuals, to be possibly associated with the high values of the α-amylase activity in this segment of the digestive tube.

Uji Kinerja Mesin Sortasi Jeruk Sistem Rotasi untuk Penyortiran Jeruk Siam Pontianak (Citrus nobilis var. microcarpa

Directory of Open Access Journals (Sweden)

Budi Setiawan

2014-10-01

Full Text Available Abstrak. Salah satu kegiatan pascapanen yang cukup menyita waktu adalah proses sortasi jeruk siam pontianak (Citrus nobilis var. microcarpa di Kabupaten Sambas. Selama ini proses sortasi ditingkat petani masih dilakukan secara manual dimana tingkat keseragaman ukuran dan tingkat kematangan rendah, standar mutu berubah-ubah dan kapasitas penyortiran rendah. Untuk mengatasi permasalahan tersebut melalui penelitian ini dikembangkan rancang bangun mesin sortasi jeruk sistem rotasi. Penelitian ini bertujuan untuk melakukan uji kinerja pada mesin sortasi jeruk sistem rotasi untuk mengetahui pengaruh kecepatan linier bidang sortasi, kemiringan bidang sortasi dan massa input buah pada mesin. Pengujian mesin dilakukan dengan 4 perlakuan kecepatan linier bidang sortasi (0,54 m/s, 0,70 m/s, 0,82 m/s dan 1,09 m/s, 3 perlakuan kemiringan bidang sortasi (10o, 20o, dan 30o dan 3 perlakuan massa input buah jeruk (2 kg, 3 kg dan 4 kg. Hasil pengujian menunjukkan bahwa massa input buah jeruk dan kecepatan linier bidang sortasi memberikan pengaruh yang signifikan terhadap kinerja mesin sortasi ini, sedangkan kemiringan bidang sortasi berpengaruh tetapi tidak signifikan. Hasil uji kinerja terbaik diperoleh pada perlakuan massa input 2 kg, kemiringan bidang sortasi 10o dan kecepatan linier bidang sortasi 1,09 m/s dengan nilai kapasitas penyortiran 1,84 kg/menit atau 110,4 kg/jam dan efisiensi penyortiran 68 %.   Performance Testing of Citrus Sorting Machine with Rotation System to Sorting Pontianak Siam Citrus (Citrus nobilis var. microcarpa Abstract. One of postharvest  activities that required much time are sorting in Sambas district. The sorting process is still done manually, where the level of uniformity of size and currently their maturity level are including the low of the quality standard fickle and sorting capacity. To overcome these problems, a study was developed to design citrus sorting machine with rotation system. This study aims to test the

A survey of homopteran species (Auchenorrhyncha from coffee shrubs and poró and laurel trees in shaded coffee plantations, in Turrialba, Costa Rica

Directory of Open Access Journals (Sweden)

Liliana Rojas

2001-12-01

Full Text Available Asurvey of homopteran species (Auchenorryncha was conducted in coffee plantations with no shade (C, and in those with shade of either poró (Erythrina poeppigiana (CP or poró plus laurel (Cordia alliodora (CPL, in Turrialba, Costa Rica. A total of 130 species in ten families were collected, dominated by Cicadellidae (82 species. Species richness was highest in the CP system (88, followed by CPL (74 and C systems (60. Five most common species for all systems were Fusigonalia lativittata, Hebralebra nicaraguensis, Neocoelidia sp., Oliarus sp. and Clastoptera sp. Diversification of the coffee agroecosystem favors some species while limiting others, and have no effect on the majority of species. Thus, only F. lativittata, Neocoelidia sp. and Scaphytopius ca. latidens were well represented in all systems, but were more abundant in coffee shrubs. Additionally, the following were the dominant species in each system: Graphocephala sp. 1 (C, F. lativittata (CP and H. nicaraguensis (CPL. Four species abundant on laurel trees, including H. nicaraguensis, appeared almost exclusively on these tree species. Species similarity was highest on the CP and CPL systems (51 % of the species in common, followed by the C and CP (39 % and the C and CPL systems (38 %. These findings show that even disturbed systems can harbor many insect species, so that they deserve attention from conservation advocatesand biologistsEn Turrialba, Costa Rica, se efectuó un inventario de especies de chicharritas (Homoptera: Auchenorryncha en plantaciones de café sin sombra (C, así como en café asociado con árboles de poró (Erythrina poeppigiana (CP o de poró y laurel (Cordia alliodora (CPL. Se recolectaron 131 especies, pertenecientes a diez familias, entre las cuales predominó Cicadellidae (82 especies. La riqueza de especies fue mayor para el sistema CP (88 especies, seguido por CPL y C, con 74 y 60 especies, respectivamente. Las cinco especies más comunes para los tres

Penialidins A-C with strong antibacterial activities from Penicillium sp., an endophytic fungus harboring leaves of Garcinia nobilis.

Science.gov (United States)

Jouda, Jean-Bosco; Kusari, Souvik; Lamshöft, Marc; Mouafo Talontsi, Ferdinand; Douala Meli, Clovis; Wandji, Jean; Spiteller, Michael

2014-10-01

Three new polyketides named penialidins A-C (1-3), along with one known compound, citromycetin (4), were isolated from an endophytic fungus, Penicillium sp., harbored in the leaves of the Cameroonian medicinal plant Garcinia nobilis. Their structures were elucidated by means of spectroscopic and spectrometric methods (NMR and HRMS(n)). The antibacterial efficacies of the new compounds (1-3) were tested against the clinically-important risk group 2 (RG2) bacterial strains of Staphylococcus aureus and Escherichia coli. The ecologically imposing strains of E. coli (RG1), Bacillus subtilis and Acinetobacter sp. BD4 were also included in the assay. Compound 3 exhibited pronounced activity against the clinically-relevant S. aureus as well as against B. subtilis comparable to that of the reference standard (streptomycin). Compound 2 was also highly-active against S. aureus. By comparing the structures of the three new compounds (1-3), it was revealed that altering the substitutions at C-10 and C-2 can significantly increase the antibacterial activity of 1. Copyright © 2014 Elsevier B.V. All rights reserved.

75 FR 43556 - TA-W-73,381, MT Rail Link, Inc., Missoula, MT; TA-W-73,381A, Billings, MT; TA-W-73,381B, Laurel...

Science.gov (United States)

2010-07-26

... DEPARTMENT OF LABOR Employment and Training Administration TA-W-73,381, MT Rail Link, Inc., Missoula, MT; TA-W-73,381A, Billings, MT; TA-W-73,381B, Laurel, MT; TA-W-73,381C, Livingston, MT; TA-W-73... Helena, Montana. The amended notice applicable to TA-W-73,381 is hereby issued as follows: All workers of...

Long-term monitoring of P. ramorum inoculum identifies spatio-temporal patterns of pathogen sporulation and proves that selective California bay laurel removal reduces risk of oak infection

Science.gov (United States)

M. Garbelotto; S. Swain; D. Schmidt

2013-01-01

In 2005, eight 50 x 50 m plots, all with a significant component of California bay laurel (Umbellularia californica (Hook. & Arn.) Nutt.), were selected in the Soquel Demonstration State Forest, Santa Cruz County, California. Each plot contained a 5 m buffer zone around the edges and sixteen 10 x 10 m squares. A bucket was placed at the center...

Induksi Embriogenesis Somatik dari Jaringan Endosperma Jeruk Siam (Citrus nobilis Lour. cv Simadu

Directory of Open Access Journals (Sweden)

Mia Kosmiatin

2014-07-01

Full Text Available ABSTRACTTriploid plants can be obtained from endosperm tissues through somatic embryogenesis regeneration. This research aimed to obtain somatic embryogenesis regeneration technique of tangerine endosperm. There were 3 experiments conducted in this research: 1 Embryogenic callus induction of tangerine endosperm. Endosperms isolated from fruits that were harvested from mother plants 11-13 weeks after anthesis and cultured on Murashige and Skoog (MS medium by modified vitamin Morel and Wetmore (MW which treated by 0.1 mg L-1 biotin, 500 mg L-1 malt extract (ME, 500 mg L-1 Casein hydrolisate (CH, 500 mg L-1 ME + 0.1 mg L-1 biotin, and 500 mg L-1CH + 0.1 mg L-1 biotin, 2 Maturation and germination of somatic embryos conducted by embryogenic callus cultured on MS medium by vitamin MW modified with addition of ABA, glutamine, and biotin, and 3 Plantlet elongation conducted on MS medium modified by MW vitamin with addition of GA3 and Kinetin. The best induction medium for embryogenic callus was modified MS enriched with 3 mg L-1 BA and 500 L-1 CH or ME, in succession 84.0 and 80.0%. The best medium for somatic embryos maturation with normal morphological plantlets (54.8% was modified MS medium without plant growth regulator with higher rate of solidified agent (from 2.5 to 3 g L-1 Phytagel. Plantlets elongation was highly (0.9 cm occurred on modified MS with enriched of 2.5 mg L-1 GA3. Keywords: Citrus nobilis (Lour., endosperm culture, in vitro, Simadu tangerine

Economic Benefit for Cuban Laurel Thrips Biological Control.

Science.gov (United States)

Shogren, C; Paine, T D

2016-02-01

The Cuban laurel thrips, Gynaikothrips ficorum Marchal (Thysanoptera: Phlaeothripidae), is a critical insect pest of Ficus microcarpa in California urban landscapes and production nurseries. Female thrips feed and oviposit on young Ficus leaves, causing the expanding leaves to fold or curl into a discolored leaf gall. There have been attempts to establish specialist predator natural enemies of the thrips, but no success has been reported. We resampled the same areas in 2013-2014 where we had released Montandoniola confusa (= morguesi) Streito and Matocq (Hemiptera: Anthocoridae) in southern California in 1995 but had been unable to recover individuals in 1997-1998. Thrips galls were significantly reduced in all three of the locations in the recent samples compared with the earlier samples. M. confusa was present in all locations and appears to be providing successful biological control. The value of the biological control, the difference between street trees in good foliage condition and trees with poor foliage, was $58,766,166. If thrips damage reduced the foliage to very poor condition, the value of biological control was $73,402,683. Total cost for the project was $61,830. The benefit accrued for every dollar spent on the biological control of the thrips ranged from $950, if the foliage was in poor condition, to $1,187, if the foliage was in very poor condition. The value of urban forest is often underappreciated. Economic analyses that clearly demonstrate the very substantial rates of return on investment in successful biological control in urban forests provide compelling arguments for supporting future efforts. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Total nitrogen and suspended-sediment loads and identification of suspended-sediment sources in the Laurel Hill Creek watershed, Somerset County, Pennsylvania, water years 2010-11

Science.gov (United States)

Sloto, Ronald A.; Gellis, Allen C.; Galeone, Daniel G.

2012-01-01

Laurel Hill Creek is a watershed of 125 square miles located mostly in Somerset County, Pennsylvania, with small areas extending into Fayette and Westmoreland Counties. The upper part of the watershed is on the Pennsylvania Department of Environmental Protection 303(d) list of impaired streams because of siltation, nutrients, and low dissolved oxygen concentrations. The objectives of this study were to (1) estimate the annual sediment load, (2) estimate the annual nitrogen load, and (3) identify the major sources of fine-grained sediment using the sediment-fingerprinting approach. This study by the U.S. Geological Survey (USGS) was done in cooperation with the Somerset County Conservation District. Discharge, suspended-sediment, and nutrient data were collected at two streamflow-gaging stations—Laurel Hill Creek near Bakersville, Pa., (station 03079600) and Laurel Hill Creek at Ursina, Pa., (station 03080000)—and one ungaged stream site, Laurel Hill Creek below Laurel Hill Creek Lake at Trent (station 03079655). Concentrations of nutrients generally were low. Concentrations of ammonia were less than 0.2 milligrams per liter (mg/L), and concentrations of phosphorus were less than 0.3 mg/L. Most concentrations of phosphorus were less than the detection limit of 0.02 mg/L. Most water samples had concentrations of nitrate plus nitrite less than 1.0 mg/L. At the Bakersville station, concentrations of total nitrogen ranged from 0.63 to 1.3 mg/L in base-flow samples and from 0.57 to 1.5 mg/L in storm composite samples. Median concentrations were 0.88 mg/L in base-flow samples and 1.2 mg/L in storm composite samples. At the Ursina station, concentrations of total nitrogen ranged from 0.25 to 0.92 mg/L in base-flow samples; the median concentration was 0.57 mg/L. The estimated total nitrogen load at the Bakersville station was 262 pounds (lb) for 11 months of the 2010 water year (November 2009 to September 2010) and 266 lb for the 2011 water year. Most of the total

Genetic and oceanographic tools reveal high population connectivity and diversity in the endangered pen shell Pinna nobilis

KAUST Repository

Wesselmann, Marlene; Gonzá lez-Wangü emert, Mercedes; Serrã o, Ester A.; Engelen, Aschwin H.; Renault, Lionel; Garcí a-March, José R.; Duarte, Carlos M.; Hendriks, Iris E.

2018-01-01

For marine meta-populations with source-sink dynamics knowledge about genetic connectivity is important to conserve biodiversity and design marine protected areas (MPAs). We evaluate connectivity of a Mediterranean sessile species, Pinna nobilis. To address a large geographical scale, partial sequences of cytochrome oxidase I (COI, 590 bp) were used to evaluate phylogeographical patterns in the Western Mediterranean, and in the whole basin using overlapping sequences from the literature (243 bp). Additionally, we combined (1) larval trajectories based on oceanographic currents and early life-history traits and (2) 10 highly polymorphic microsatellite loci collected in the Western Mediterranean. COI results provided evidence for high diversity and low inter-population differentiation. Microsatellite genotypes showed increasing genetic differentiation with oceanographic transport time (isolation by oceanographic distance (IBD) set by marine currents). Genetic differentiation was detected between Banyuls and Murcia and between Murcia and Mallorca. However, no genetic break was detected between the Balearic populations and the mainland. Migration rates together with numerical Lagrangian simulations showed that (i) the Ebro Delta is a larval source for the Balearic populations (ii) Alicante is a sink population, accumulating allelic diversity from nearby populations. The inferred connectivity can be applied in the development of MPA networks in the Western Mediterranean.

Genetic and oceanographic tools reveal high population connectivity and diversity in the endangered pen shell Pinna nobilis

KAUST Repository

Wesselmann, Marlene

2018-03-13

For marine meta-populations with source-sink dynamics knowledge about genetic connectivity is important to conserve biodiversity and design marine protected areas (MPAs). We evaluate connectivity of a Mediterranean sessile species, Pinna nobilis. To address a large geographical scale, partial sequences of cytochrome oxidase I (COI, 590 bp) were used to evaluate phylogeographical patterns in the Western Mediterranean, and in the whole basin using overlapping sequences from the literature (243 bp). Additionally, we combined (1) larval trajectories based on oceanographic currents and early life-history traits and (2) 10 highly polymorphic microsatellite loci collected in the Western Mediterranean. COI results provided evidence for high diversity and low inter-population differentiation. Microsatellite genotypes showed increasing genetic differentiation with oceanographic transport time (isolation by oceanographic distance (IBD) set by marine currents). Genetic differentiation was detected between Banyuls and Murcia and between Murcia and Mallorca. However, no genetic break was detected between the Balearic populations and the mainland. Migration rates together with numerical Lagrangian simulations showed that (i) the Ebro Delta is a larval source for the Balearic populations (ii) Alicante is a sink population, accumulating allelic diversity from nearby populations. The inferred connectivity can be applied in the development of MPA networks in the Western Mediterranean.

[A new strategy for enhancing acanthamoebicidal activity with synthesis of nanoflower of Laurocerausus officinalis Roemer (cherry laurel) fruit extracts].

Science.gov (United States)

Baldemir, Ayşe; Karaman, Ülkü; Yusufbeyoğlu, Sadi; Eken, Ayşe; Ildız, Nilay; İlgün, Selen; Çolak, Cemil; Kaçmaz, Gamze; Öçsoy, İsmail; Çankaya, Soner

2018-01-01

Pathogenic Acanthamoeba species often cause infection known as Acanthamoeba keratitis among people who use contact lenses. It is a type of infection that can result in corneal ulceration, visual loss or even blindness, if not treated. There are various therapeutic options available in the treatment of Acanthamoeba infections but they are usually tough treatments with limited efficacy. For instance, hydrogen peroxide (H 2 O 2 ) is a commonly used contact lens disinfectant which is effective against Acanthamoeba but it is toxic to the cornea. For these reasons, new and more efficacious treatment options are required for Acanthamoeba infections. In this context, plants are considered natural resources for the discovery of new drugs. Laurocerasus officinalis Roem. (cherry laurel) (Rosaceae) grows in Black Sea region; and it is known as "Taflan", "Laz kirazı" or "Karayemis". Local people are using the seeds against diabetes, while the fruits are consuming as food, and used fordiuretic and passing kidney stones. It has also been reported that the seeds of the cherry laurel are used as an antiparasitic agent in this area. The aim of the study was to confirm the traditionally use of antiparasitic activity of this fruit and to increase the potential effect by means of organic-inorganic hybrid synthesis. Total phenol contents of methanol extracts prepared from endocarp, mesocarp and seeds of the fruit were calculated. The effects of methanol extracts and nano flower (NFs) plants synthesized from these extracts on the proliferation of Acanthamoeba castellanii were investigated. Thus, for the first time, novel organic-inorganic nanobio-antiparasitic agents called NFs were produced from cherry laurel and the increase in the amoebicidal activity of the NFs was elucidated. The characterization of NFs were determined with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometer (FT-IR) and Energy-Dispersive X-ray (EDX) techniques. In addition, the catalytic

Effect of chipping on emergence of the redbay ambrosia beetle (Coleoptera: Curculionidae: Scolytinae) and recovery of the laurel wilt pathogen from infested wood chips.

Science.gov (United States)

Spence, D J; Smith, J A; Ploetz, R; Hulcr, J; Stelinski, L L

2013-10-01

Significant mortality ofredbay trees (Persea borbonia (L.) Spreng.) in the southeastern United States has been caused by Raffaelea lauricola, T.C. Harr., Fraedrich, & Aghayeva (Harrington et al. 2008), a fungal symbiont of the exotic redbay ambrosia beetle, Xyleborus glabratus, Eichhoff (Fraedrich et al. 2008). This pathogen causes laurel wilt, which is an irreversible disease that can kill mature trees within a few weeks in summer. R. lauricola has been shown to be lethal to most native species of Lauraceae and cultivated avocado (Persea americana Mill.) in the southeastern United States. In this study, we examined the survival of X. glabratus and R. lauricola in wood chips made from infested trees by using a standard tree chipper over a 10-wk period. After 2 wk, 14 X. glabratus were recovered from wood chips, whereas 339 X. glabratus emerged from nonchipped bolts. R. lauricola was not found 2 d postchipping from wood chips, indicating that the pathogen is not likely to survive for long inside wood chips. In contrast, R. lauricola persisted in dead, standing redbay trees for 14 mo. With large volumes of wood, the potential for infested logs to be moved between states or across U.S. borders is significant. Results demonstrated that chipping wood from laurel wilt-killed trees can significantly reduce the number of X. glabratus and limit the persistence of R. lauricola, which is important for sanitation strategies aimed at limiting the spread of this disease.

OPTIMIZATION OF STIRRING SPEED AND STIRRING TIME TOWARD NANOPARTICLE SIZE OF CHITOSAN-SIAM CITRUS PEEL (Citrus nobilis L.var Microcarpa 70% ETHANOL EXTRACT

Directory of Open Access Journals (Sweden)

Wintari Taurina

2017-04-01

Full Text Available Siam citrus peel (Citrus nobilis L. var. Microcarpa is a plant derived from Sambas Regency, West Kalimantan Province. Bioavailability of herbal active compounds can be enhanced by formulating extract into nanoparticle. The polymer used was chitosan with crosslinker Na-TPP. Stirring speed and stirring time play an important role to produce small particle size in forming nanoparticle using ionic gelation method. Enhancement of stirring speed and stirring time could reduce particle size. Nanoparticles were prepared using ionic gelation method by mixing Na-TPP, extract and chitosan (1:1:6 with varying the stirring speed 500 rpm, 1000 rpm, 1500 rpm and stirring time 1 hrs, 2 hrs, 3 hrs. The particle size of nanoparticle was found to be 85.3 nm at 1000 rpm of stirring speed and 3 hrs of stirring times, with polidispersity index 0.287, zeta potential +32.37 mV and entrapment efficiency 87.12 %.

A reconstruction of Palaeo-Macaronesia, with particular reference to the long-term biogeography of the Atlantic island laurel forests

DEFF Research Database (Denmark)

Fernández-Palacios, José María; de Nascimento, Lea; Otto, Rüdiger

2011-01-01

Macaronesia is a biogeographical region comprising five Atlantic Oceanic archipelagos: the Azores, Madeira, Selvagen (Savage Islands), Canaries and Cape Verde. It has strong affinities with the Atlantic coast of the Iberian Peninsula and the north-western fringes of Africa. This paper re...... the role of these archipelagos as stepping stones and as both repositories of palaeo-endemic forms and crucibles of neo-endemic radiations of plant and animal groups. Our principal focus is on the laurel forest communities, long considered impoverished relicts of the Palaeotropical Tethyan flora....... This account is therefore contextualized by reference to the long-term climatic and biogeographical history of Southern Europe and North Africa and by consideration of the implications of changes in land–sea configuration, climate and ocean circulation for Macaronesian biogeography. We go on to provide...

In Vitro Pollen Viability and Pollen Germination in Cherry Laurel (Prunus laurocerasus L.

Directory of Open Access Journals (Sweden)

Melekber Sulusoglu

2014-01-01

Full Text Available Pollen quality is important for growers and breeders. This study was carried out to determine in vitro pollen viability and pollen germination in seven genotypes of cherry laurel (Prunus laurocerasus L.. Two pollen viability tests, TTC (2,3,5-triphenyl tetrazolium chloride and IKI (iodine potassium iodide, were used. Pollen traits of genotypes were studied using an in vitro medium containing 0%, 5%, 10%, 15%, and 20% sucrose to determine the best sucrose concentrations for germination. In the second step, the germinated pollen was counted 1, 4, 6, 10, 12, 24, and 48 hours later until there was no further germination. The viability rates were different according to genotypes and tests used. The IKI and TTC staining tests and pollen germination had low correlation (r2 = 0.0614 and r2 = 0.0015, resp.. Painted pollen rate was higher and pollen was well-stained with IKI test and pollen viability estimated with TTC staining test was better than that estimated with the IKI staining test. 15% sucrose gave the best germination rates in most of the genotypes. Pollen germination rates were recorded periodically from one hour to 48 hours in 15% sucrose and the results showed that pollen germination rates increased after 6 hours of being placed in culture media.

In vitro pollen viability and pollen germination in cherry laurel (Prunus laurocerasus L.).

Science.gov (United States)

Sulusoglu, Melekber; Cavusoglu, Aysun

2014-01-01

Pollen quality is important for growers and breeders. This study was carried out to determine in vitro pollen viability and pollen germination in seven genotypes of cherry laurel (Prunus laurocerasus L.). Two pollen viability tests, TTC (2,3,5-triphenyl tetrazolium chloride) and IKI (iodine potassium iodide), were used. Pollen traits of genotypes were studied using an in vitro medium containing 0%, 5%, 10%, 15%, and 20% sucrose to determine the best sucrose concentrations for germination. In the second step, the germinated pollen was counted 1, 4, 6, 10, 12, 24, and 48 hours later until there was no further germination. The viability rates were different according to genotypes and tests used. The IKI and TTC staining tests and pollen germination had low correlation (r(2) = 0.0614 and r(2) = 0.0015, resp.). Painted pollen rate was higher and pollen was well-stained with IKI test and pollen viability estimated with TTC staining test was better than that estimated with the IKI staining test. 15% sucrose gave the best germination rates in most of the genotypes. Pollen germination rates were recorded periodically from one hour to 48 hours in 15% sucrose and the results showed that pollen germination rates increased after 6 hours of being placed in culture media.

Occurrence of Radium-224, Radium-226 and Radium-228 in Water from the Vincentown and Wenonah-Mount Laurel Aquifers, the Englishtown Aquifer System, and the Hornerstown and Red Bank Sands, Southwestern and South-Central New Jersey

Science.gov (United States)

dePaul, Vincent T.; Szabo, Zoltan

2007-01-01

This investigation is the first regionally focused study of the presence of natural radioactivity in water from the Vincentown and Wenonah-Mount Laurel aquifers, Englishtown aquifer system, and the Hornerstown and Red Bank Sands. Geologic materials composing the Vincentown and Wenonah-Mount Laurel aquifers and the Hornerstown and Red Bank Sands previously have been reported to contain radioactive (uranium-enriched) phosphatic strata, which is common in deposits from some moderate-depth coastal marine environments. The decay of uranium and thorium gives rise to natural radioactivity and numerous radioactive progeny, including isotopes of radium. Naturally occurring radioactive isotopes, especially those of radium, are of concern because radium is a known human carcinogen and ingestion (especially in water used for drinking) can present appreciable health risks. A regional network in southwestern and south-central New Jersey of 39 wells completed in the Vincentown and Wenonah-Mount Laurel aquifers, the Englishtown aquifer system, and the Hornerstown and Red Bank Sands was sampled for determination of gross alpha-particle activity; concentrations of radium radionuclides, major ions, and selected trace elements; and physical properties. Concentrations of radium-224, radium-226, and radium-228 were determined for water from 28 of the 39 wells, whereas gross alpha-particle activity was determined for all 39. The alpha spectroscopic technique was used to determine concentrations of radium-224, which ranged from less than 0.5 to 2.7 pCi/L with a median concentration of less than 0.5pCi/L, and of radium-226, which ranged from less than 0.5 to 3.2 pCi/L with a median concentration of less than 0.5 pCi/L. The beta-counting technique was used to determine concentrations of radium-228. The concentration of radium-228 ranged from less than 0.5 to 4.3 pCi/L with a median of less than 0.5. Radium-228, when quantifiable, had the greatest concentration of the three radium

Photonic Crystals with Large Complete Bandgap Composed of an Approximately Ordered Array of Laurel-Crown-Like Structures Fabricated by Employing Anodic Aluminum Oxide Template

Science.gov (United States)

Chan, Der-Sheng; Chau, Yuan-Fong

2013-01-01

An innovative fabrication processes of a photonic crystal composed of an approximately ordered array of laurel-crown-like structures by employing an anodic aluminum oxide (AAO) template is presented. We found that the intensity of the electric field is affected by the microstructure and surface morphology of aluminum foil after etching the scalloped barrier oxide layer (BOL). In addition, the electric current is strongly dependent on the electric field distribution in the scalloped BOL at the pore bottoms. By using a different step potential (DSP) of 30-60 V in series, the proposed photonic crystal is fabricated and possesses a large complete photonic bandgap.

The Effect of Fish Size and Condition on the Contents of Twelve Essential and Non Essential Elements in Aristichthys nobilis

Directory of Open Access Journals (Sweden)

M. Naeem*, A. Salam, S. S. Tahir1 and N. Rauf 1

2011-04-01

Full Text Available The correlation coefficients between fish size (body weight and total length and metal contents (Na, K, Ca, Mg, Mn, Fe, Cu, Zn, Cr, Co, Cd and Pb in whole fish (Aristichthys nobilis were determined. A total of 71 fish samples were collected from hatcheries and fish reservoirs located in Islamabad and Fatehjung. Highly significant (P<0.001 relationship between metal concentrations and fish size was found. Most of the metals (Na, K, Ca, Mg, Cu, Zn, Cr, Cd and Pb showed an isometric increase, while Mn, Fe and Co showed an allometric increase in with increasing body weight. All metals showed isometric increase, while, Na, Mn, Fe, Cu, and Co showed positive allometric growth in relation to total length. The correlation coefficient (r between different variables and wet body weight, condition factor was found highly significant (P<0.001 in examined fish except for Na, Ca, Cu, Zn, Cd and Pb while for total length the same results found except Ca, Cd, Zn and Pb. Variance inflation factor values of regression coefficients in multiple regression analysis for each variable were lesser than 10. The metal levels of the examined fish were lower than the recommended values in fish and fishery products set by FAO.

Rearing conditions and habitat use of white seabass (Atractoscion nobilis) in the northeastern Pacific based on otolith isotopic composition

Science.gov (United States)

Romo-Curiel, Alfonsina E.; Herzka, Sharon Z.; Sepulveda, Chugey A.; Pérez-Brunius, Paula; Aalbers, Scott A.

2016-03-01

White seabass, Atractoscion nobilis, is an important coastal resource throughout both California and Baja California, but whether this species comprises a single or multiple subpopulations in the northeastern Pacific is not known. The aim of this study was to infer larval rearing habitats and population structure of white seabass by sampling adults from three regions spanning a latitudinal temperature gradient and a distance of over 1000 km, and analyzing the isotopic composition (δ18O and δ13C) of otolith aragonite corresponding to the larval, juvenile and adult stages. Otolith cores revealed high isotopic variability and no significant differences among regions, suggesting overlapping rearing conditions during the larval stage, the potential for long distance dispersal or migration or selective mortality of larvae at higher temperatures. Back-calculated temperatures of aragonite precipitation derived using regional salinity-δw relationships and local salinity estimates also did not differ significantly. However, there were significant differences between the δ18O values of the first seasonal growth ring of age 0 fish as well as back-calculated aragonite precipitation temperatures, suggesting the presence of two potentially discrete subpopulations divided by Punta Eugenia (27°N) along the central Baja California peninsula. These findings are consistent with regional oceanographic patterns and are critical for understanding white seabass population structure, and provide information needed for the implementation of appropriate management strategies.

Quantification of eDNA shedding rates from invasive bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix

Science.gov (United States)

Klymus, Katy E.; Richter, Catherine A.; Chapman, Duane C.; Paukert, Craig P.

2015-01-01

Wildlife managers can more easily mitigate the effects of invasive species if action takes place before a population becomes established. Such early detection requires sensitive survey tools that can detect low numbers of individuals. Due to their high sensitivity, environmental DNA (eDNA) surveys hold promise as an early detection method for aquatic invasive species. Quantification of eDNA amounts may also provide data on species abundance and timing of an organism’s presence, allowing managers to successfully combat the spread of ecologically damaging species. To better understand the link between eDNA and an organism’s presence, it is crucial to know how eDNA is shed into the environment. Our study used quantitative PCR (qPCR) and controlled laboratory experiments to measure the amount of eDNA that two species of invasive bigheaded carps (Hypophthalmichthys nobilis and Hypophthalmichthys molitrix) shed into the water. We first measured how much eDNA a single fish sheds and the variability of these measurements. Then, in a series of manipulative lab experiments, we studied how temperature, biomass (grams of fish), and diet affect the shedding rate of eDNA by these fish. We found that eDNA amounts exhibit a positive relationship with fish biomass, and that feeding could increase the amount of eDNA shed by ten-fold, whereas water temperature did not have an effect. Our results demonstrate that quantification of eDNA may be useful for predicting carp density, as well as densities of other rare or invasive species.

Antifungal activity and ultrastructural alterations in Pseudocercospora griseola treated with essential oils Atividade antifúngica e alterações ultraestruturais em Pseudocercospora griseola tratado com óleos essenciais

Directory of Open Access Journals (Sweden)

Julián Mauricio Ágredo Hoyos

2012-06-01

Full Text Available Pseudocercospora griseola, the etiologic agent of angular leaf spot of common bean (Phaseolus vulgaris, is an important disease in all bean-producing regions worldwide and may cause extremely high yield losses. The control of this disease is made more difficult by the pathogen's genetic variability and the inefficiency of fungicides. In this study, of 26 essential oils tested at different concentrations, 25 demonstrated efficiency in affecting the germination of strains 63-31 and 63-63 of the pathogen, reaching inhibition levels of between 80% and 100%. Cymbopogon citratus and Cymbopogon martinii inhibited conidia germination at all concentrations; Eugenia caryophyllata, Cinnamomum sp., Thymus vulgaris, Matricaria recutita, Cordia verbenacea, Origanum vulgare, Cymbopogon nardus, at 0.1 and 0.5%; and Zingiber officinale, Mentha arvensis, Chamaecyparis pisifera, Lavandula officinalis, Ocimum basilicum, Pimpinella anisum, Ocimum selloi, Baccharis dracunculifolia, Laurus nobilis, Citrus sinensis, Melaleuca alternifolia and Eucalyptus globulus, at 0.5%. The main constituents identified were cinnamaldehyde in Cinnamomum sp.; eugenol in E. caryophyllata; trans-β-farnesene in M. recutita; pulegone in C. verbenacea; thymol in T. vulgaris; geranial and neral in C. citratus, and geraniol in C. martini. Through transmission electron microscopy (TEM, it was verified that C. citratus, C. martini and E. caryophyllata presented direct fungitoxic action on P. griseola, causing severe damage to the cellular ultrastructure of the conidia, invalidating germination. These results indicated that essential oils are a promising alternative strategy for the control of angular leaf spot in bean, representing less risk to human health and the environment.Pseudocercospora griseola, agente etiológico da mancha angular do feijoeiro comum (Phaseolus vulgaris, é uma doença importante nas regiões produtoras de feijão em todo o mundo e pode causar perdas de produtividade

TRADITIONAL USE OF A PROTECTED SPECIES (Litsea glaucescens Kunth IN THE “SIERRA DEL LAUREL

Directory of Open Access Journals (Sweden)

Salvador Martín Medina-Torres

2011-05-01

Full Text Available Whenever a natural area is proposed to be a protected area, the information that supports the proposal is usually based on the physical environmental, rather than on the social environment and on the management of natural resource. This study was conducted from May 2004 to June 2005 whit the objective to analyze the process of exploitation of Mexican Bay Leaf (Litsea glaucescens Kunth in rural communities of Sierra del Laurel, a priority area in the state of Aguascalientes, México. Thirteen semi-structured interview were applied for local collectors and seven more were applied to inhabitants of the region to understand the trends in the use of resource. Three interviews were also applied to environmental officials from federal and local institutions to know about their position regarding the use of the Mexican Bay Leaf. A participative workshop with producers and collectors was conducted to document their opinion on the status of the population of this specie. Trend in utilization of the Mexican Bay Leaf have led it to be in danger of extinction: there is not organization nor legal utilization schemes among collectors that allow regeneration; the lack of institutional coordination, along whit inefficient implementation of laws and other legal instruments to regulate the management of natural resources, has contributed so that users have not adequate risk management procedures.

Surveying the Effect of the Phenol Compounds on Antibacterial Activity of Herbal Extracts: In vitro Assessment of Herbal Extracts in Fasa-Fars Province

Directory of Open Access Journals (Sweden)

Elahe Ahmadi

2016-09-01

Full Text Available Background & Objectives: Due to increase in  bacterial drug resistance, discovering new antibacterial compounds is really important. The objective of this study is to evaluate the phenol compounds effect on antibacterial activity of herbal extracts of Fasa-Fars province in vitro. Materials & Methods: The antibacterial activity of 26 plants was studied by disk diffusion, well, and MIC methods in compare with 13 standard antibiotics against S. aureus and E. coli as control bacteria. Measurement of phenol compounds were performed by Seevers and Daly colorimetric methods using Folin-ciocalteu indicator. Results: Inhibition zone of bacterial growth  against S. aureus in well and disk methods were 32 and 22 mm in using Zataria multiflora, respectively .And there were 23 and 16 mm against E. coli in Zataria multiflora, respectively. Less effects and inhibition zones, less than 15mm on both strains, were seen in using  Saturina hortensis, Cinamomum zeylanicum, ­Artemisia absinthium, ­Urtica dioica, Carum carvi L. cyminum Cuminum, Achillea fragrantissimia, Marticaria chamomilla, Zingiber officinale, Origanum majorana, and Plantago psyllium. Most effective MIC results, 7.8 µg/ml, were related to the extracts of Zataria multiflora, Carum copticum L. Rosmarinus officinalis L., and Laurus nobilis L. Phenol compound amounts were approximately between 66.51±1.9 and 233.15±5.1 mg/gr extract in Zataria multiflora and Plantago psyllium, respectively. Conclusion: Results of antibacterial activity of extracts and relation with phenol compound amounts indicate the antibacterial effect of phenol compounds in herbal extracts.

Antibacterial, anti-swarming and anti-biofilm formation activities of Chamaemelum nobile against Pseudomonas aeruginosa.

Science.gov (United States)

Kazemian, Hossein; Ghafourian, Sobhan; Heidari, Hamid; Amiri, Pouya; Yamchi, Jalil Kardan; Shavalipour, Aref; Houri, Hamidreza; Maleki, Abbas; Sadeghifard, Nourkhoda

2015-01-01

Chamomile (Chamaemelum nobile) is widely used throughout the world, and has anti-inflammatory, deodorant, bacteriostatic, antimicrobial, carminative, sedative, antiseptic, anti-catarrhal, and spasmolytic properties. Because of the increasing incidence of drug-resistant bacteria, the development of natural antibacterial sources such as medical herbs for the treatment of infectious diseases is necessary. Extracts from different plant parts such as the leaves, flowers, fruit, and bark of Combretum albiflorum, Laurus nobilis , and Sonchus oleraceus were found to possess anti-quorum sensing (QS) activities. In this study, we evaluated the effect of C. nobile against Pseudomonas aeruginosa biofilm formation. The P. aeruginosa samples were isolated from patients with different types of infection, including wound infection, septicemia, and urinary tract infection. The flowers of C. nobile were dried and the extract was removed using a rotary device and then dissolved in dimethyl sulfoxide at pH 7.4. The microdilution method was used to evaluate the minimum inhibitory concentration (MIC) of this extract on P. aeruginosa , and biofilm inhibition was assayed. Eighty percent of the isolated samples (16/20) could form a biofilm, and most of these were isolated from wound infections. The biofilm inhibitory concentration of the C. nobile extract was 6.25-25mg/ml, whereas the MIC was 12.5-50mg/ml. The anti-QS property of C. nobile may play an important role in its antibacterial activity, thus offering an additional strategy in the fight against bacterial infections. However, molecular investigation is required to explore the exact mechanisms of the antibacterial action and functions of this phytocompound.

Descomposición y liberación de nutrimentos desde las raíces finas de los sistemas agroforestales de cacao con sombras de poró y laurel, en Turrialba, Costa Rica Descomposición y liberación de nutrimentos desde las raíces finas de los sistemas agroforestales de cacao con sombras de poró y laurel, en Turrialba, Costa Rica

Directory of Open Access Journals (Sweden)

Muñoz Arboleda Fernando

2006-06-01

Full Text Available

Se usó el método de bolsas de descomposición para estudiar la pérdida de biomasa y liberación de N, P, K, Ca y Mg desde las raíces finas (<2 mm en los sistemas agroforestales de cacao con sombras de poró y laurel. Se muestrearon bolsas a las 1, 2, 4, 6, 12, 18 y 25 semanas en las épocas seca y lluviosa. Las tasas de descomposición y liberación de nutrimentos fueron bien descritas por un modelo exponencial doble. Se observó alta relación entre las tasas de pérdida de biomasa y liberación de N de las tres especies. Las tasas de liberación de nutrimentos fueron K > Mg > P > N > Ca. Después de una semana de incubación cerca del 90% del K fue liberado mientras que el Ca tendió a la acumulación. Las raíces de poró fueron las de más rápida descomposición y liberación de nutrimentos, excepto de K. El poró sería la especie de sombra adecuada para sistemas de producción que requieran rápida recirculación de nutrimentos. El laurel exhibió tasas lentas de descomposición y liberación de nutrimentos.

Palabras claves: Agroforestería, Theobroma cacao, Erythrina poeppigiana, Cordia alliodora, recirculación de nutrimentos, descomposición de raíces finas.

Xyleborus bispinatus Reared on Artificial Media in the Presence or Absence of the Laurel Wilt Pathogen (Raffaelea lauricola).

Science.gov (United States)

Menocal, Octavio; Cruz, Luisa F; Kendra, Paul E; Crane, Jonathan H; Cooperband, Miriam F; Ploetz, Randy C; Carrillo, Daniel

2018-02-28

Like other members of the tribe Xyleborini, Xyleborus bispinatus Eichhoff can cause economic damage in the Neotropics. X. bispinatus has been found to acquire the laurel wilt pathogen Raffaelea lauricola (T. C. Harr., Fraedrich & Aghayeva) when breeding in a host affected by the pathogen. Its role as a potential vector of R. lauricola is under investigation. The main objective of this study was to evaluate three artificial media, containing sawdust of avocado ( Persea americana Mill.) and silkbay ( Persea humilis Nash.), for rearing X. bispinatus under laboratory conditions. In addition, the media were inoculated with R. lauricola to evaluate its effect on the biology of X. bispinatus . There was a significant interaction between sawdust species and R. lauricola for all media. Two of the media supported the prolific reproduction of X. bispinatus , but the avocado-based medium was generally more effective than the silkbay-based medium, regardless whether or not it was inoculated with R. lauricola . R. lauricola had a neutral or positive effect on beetle reproduction. The pathogen was frequently recovered from beetle galleries, but only from a few individuals which were reared on inoculated media, and showed limited colonization of the beetle's mycangia. Two media with lower water content were most effective for rearing X. bispinatus .

Handling Practices During Distribution of Kinnow-Mandarins (Citrus nobilis x Citrus deliciosa Used for Preparation of Fresh-Squeezed Juices and their Effects on Microbiological Safety

Directory of Open Access Journals (Sweden)

M. Ghosh

2005-01-01

Full Text Available The objective of this study was to assess the microbiological safety of Kinnow-mandarins (Citrus nobilis X Citrus deliciosa used for preparation of fresh squeezed juices by street vendors. One hundred and fifty Kinnow mandarin samples were collected from different points in the distribution chain from the same lot, washed and aliquots of the wash samples were analyzed for total aerobes, Staphylococcus, total and fecal coliforms, Salmonella and Shigella. Although, there was no notable increase in the total aerobic, total or fecal coliform counts, total staphylococcal counts increased significantly (P<0.05 during distribution to street vendors; seventy-two samples from the street vendors showed the presence of coagulase positive Staphylococcus aureus, twenty-three of these produced enterotoxins B and C. Salmonella and Shigella were not detected in any of the samples. Sources of high numbers of enterotoxigenic S. aureus were traced to unhygienic manual handling by middle level buyers and by street vendors. The results of our study demonstrate the poor microbiological quality of Kinnow-mandarins, the possible entry points of contaminants in the distribution chain of these fruits and unhygienic vending practices. Appropriate intervention measures are needed to ensure safe fresh squeezed juices for consumers.

[Simultaneous determination of five alkaloids in Niuhuang Shangqing Tablets by micellar electrokinetic chromatography-mass spectrometry using laurel acyl malic acid ester].

Science.gov (United States)

Hu, Mengdi; Su, Di; Fan, Xiaosu; Yu, Jianhua; Xu, Yuanjin

2012-12-01

A micellar electrokinetic chromatography-mass spectrometric method based on laurel acyl malic acid ester (LMAE) for the separation and determination of coptisine, berberine, jatrorrhizine, phellodendrine and ligustrazine in Niuhuang Shangqing Tablets was established. The baseline separation of the five compounds was attained within 18 min by an uncoated capillary (88 cm x 50 microm) on the operating voltage of 25 kV using 7.5 mmol/L LMAE-15 mmol/L ammonia-50 mmol/L ammonium acetate mixture (pH = 7.0) containing 12.5% (v/v) acetonitrile as the electrophoretic medium and 50% 2-propanol aqueous solution (containing 3 mmol/L acetic acid) as the sheath liquid. The peak area of each component to its concentration showed a good linear relationship. The relative standard deviations of migration times and peak areas of the five components were less than 5% and the recoveries were between 96.0% and 105%. The developed method is simple, rapid, accurate and is suitable for the routine analysis of the five alkaloid components in Niuhuang Shangqing Tablets.

Biogeography of Mediterranean Hotspot Biodiversity: Re-Evaluating the 'Tertiary Relict' Hypothesis of Macaronesian Laurel Forests.

Science.gov (United States)

Kondraskov, Paulina; Schütz, Nicole; Schüßler, Christina; de Sequeira, Miguel Menezes; Guerra, Arnoldo Santos; Caujapé-Castells, Juli; Jaén-Molina, Ruth; Marrero-Rodríguez, Águedo; Koch, Marcus A; Linder, Peter; Kovar-Eder, Johanna; Thiv, Mike

2015-01-01

The Macaronesian laurel forests (MLF) are dominated by trees with a laurophyll habit comparable to evergreen humid forests which were scattered across Europe and the Mediterranean in the Paleogene and Neogene. Therefore, MLF are traditionally regarded as an old, 'Tertiary relict' vegetation type. Here we address the question if key taxa of the MLF are relictual. We evaluated the relict hypothesis consulting fossil data and analyses based on molecular phylogenies of 18 representative species. For molecular dating we used the program BEAST, for ancestral trait reconstructions BayesTraits and Lagrange to infer ancestral areas. Our molecular dating showed that the origins of four species date back to the Upper Miocene while 14 originated in the Plio-Pleistocene. This coincides with the decline of fossil laurophyllous elements in Europe since the middle Miocene. Ancestral trait and area reconstructions indicate that MLF evolved partly from pre-adapted taxa from the Mediterranean, Macaronesia and the tropics. According to the fossil record laurophyllous taxa existed in Macaronesia since the Plio- and Pleistocene. MLF are composed of species with a heterogeneous origin. The taxa dated to the Pleistocene are likely not 'Tertiary relicts'. Some species may be interpreted as relictual. In this case, the establishment of most species in the Plio-Pleistocene suggests that there was a massive species turnover before this time. Alternatively, MLF were largely newly assembled through global recruitment rather than surviving as relicts of a once more widespread vegetation. This process may have possibly been triggered by the intensification of the trade winds at the end of the Pliocene as indicated by proxy data.

Peynirlere Kontamine Olan Küflerin Bazı Esansiyel Yağlar ile İnhibisyonu

Directory of Open Access Journals (Sweden)

Sibel Özçakmak

2015-02-01

Full Text Available Küf kontaminasyonu önemli kalite problemlerine ve peynir üreticilerine ekonomik kayıplara neden olmaktadır. Peynirlerden yaygın olarak izole edilen kontamine küf türleri Penicillium commune, P. verrucosum, P. roqueforti, P. palitans, Aspergillus flavus ve Geotrichum candidum olarak belirlenmiştir. Sorbik asit veya bunların tuzları, bozulma etmeni küflerin önlenmesi için peynir yüzeylerine uygulanmaktadır. Bazı sentetik gıda katkılarının kullanımıyla ilgili gıda endüstrisi ve mevzuatı oluşturan kuruluşlar tarafından getirilen kısıtlamalar doğal antimikrobiyel bileşikler üzerine araştırmaların doğmasına neden olmuştur. Bitki ve baharat, gıdaların mikrobiyolojik güvenliğini sağlayan etkili bileşiklere sahiptir. Salvia officinalis (ada çayı, Laurus nobilis (defne, Cinnamomum zeylanicum (tarçın, Thymus vulgaris (kekik, Cymbopogon citratus (limon otu, Origanum vulgare (keklik otu esansiyel yağları, bozulma etmeni küf şorasını inhibe edebilmiştir. Thymol ve karanfil esansiyel yağlarının Çedar peynirinde P. citrinum, Kaşar peynirinde A. flavus; zeytin yaprağı ekstraklarının, Tulum peynirinde A. niger, P. citrinum ve P. roqueforti, beyaz peynirde Mucor rocemosus’un gelişimini inhibe edebildiği bildirilmiştir.

Antibacterial, anti-swarming and anti-biofilm formation activities of Chamaemelum nobile against Pseudomonas aeruginosa

Directory of Open Access Journals (Sweden)

Hossein Kazemian

2015-08-01

Full Text Available AbstractINTRODUCTION:Chamomile ( Chamaemelum nobile is widely used throughout the world, and has anti-inflammatory, deodorant, bacteriostatic, antimicrobial, carminative, sedative, antiseptic, anti-catarrhal, and spasmolytic properties. Because of the increasing incidence of drug-resistant bacteria, the development of natural antibacterial sources such as medical herbs for the treatment of infectious diseases is necessary. Extracts from different plant parts such as the leaves, flowers, fruit, and bark of Combretum albiflorum, Laurus nobilis , and Sonchus oleraceus were found to possess anti-quorum sensing (QS activities. In this study, we evaluated the effect of C. nobile against Pseudomonas aeruginosa biofilm formationMETHODS:The P. aeruginosa samples were isolated from patients with different types of infection, including wound infection, septicemia, and urinary tract infection. The flowers of C. nobile were dried and the extract was removed using a rotary device and then dissolved in dimethyl sulfoxide at pH 7.4. The microdilution method was used to evaluate the minimum inhibitory concentration (MIC of this extract on P. aeruginosa , and biofilm inhibition was assayed.RESULTS:Eighty percent of the isolated samples (16/20 could form a biofilm, and most of these were isolated from wound infections. The biofilm inhibitory concentration of the C. nobile extract was 6.25-25mg/ml, whereas the MIC was 12.5-50mg/ml.CONCLUSIONS:The anti-QS property of C. nobile may play an important role in its antibacterial activity, thus offering an additional strategy in the fight against bacterial infections. However, molecular investigation is required to explore the exact mechanisms of the antibacterial action and functions of this phytocompound.

α-Terpineol attenuates morphine-induced physical dependence and tolerance in mice: role of nitric oxide.

Science.gov (United States)

Parvardeh, Siavash; Moghimi, Mahsa; Eslami, Pegah; Masoudi, Alireza

2016-02-01

Dependence and tolerance to opioid analgesics are major problems limiting their clinical application. α-Terpineol is a monoterpenoid alcohol with neuroprotective effects which is found in several medicinal plants such as Myrtus communis, Laurus nobilis, and Stachys byzantina. It has been shown that some of these medicinal plants such as S. byzantina attenuate dependence and tolerance to morphine. Since α-terpineol is one of the bioactive phytochemical constituent of these medicinal plants, the present study was conducted to investigate the effects of α-terpineol on morphine-induced dependence and tolerance in mice. The mice were rendered dependent or tolerant to morphine by a 3-day administration schedule. The hot-plate test and naloxone-induced withdrawal syndrome were used to evaluate tolerance and dependence on morphine, respectively. To investigate a possible role for nitric oxide (NO) in the protective effect of α-terpineol, the NO synthase inhibitor, L-N(G)-nitroarginine methyl ester (L-NAME) and NO precursor, L-arginine, were used. Administration of α-terpineol (5, 10, and 20 mg/kg, IP) significantly decreased the number of jumps in morphine dependent animals. Moreover, α-terpineol (20 and 40 mg/kg, IP) attenuated tolerance to the analgesic effect of morphine. The inhibitory effects of α-terpineol on morphine-induced dependence and tolerance were enhanced by pretreatment with L-NAME (10 mg/kg, IP). However, L-arginine (300 mg/kg, IP) antagonized the protective effects of α-terpineol on dependence and tolerance to morphine. These findings indicate that α-terpineol prevents the development of dependence and tolerance to morphine probably through the influence on NO production.

In Vitro Antibacterial Activity of Several Plant Extracts and Oils against Some Gram-Negative Bacteria

Directory of Open Access Journals (Sweden)

Ayman Al-Mariri

2014-01-01

Full Text Available Background: Medicinal plants are considered new resources for producing agents that could act as alternatives to antibiotics in the treatment of antibiotic-resistant bacteria. The aim of this study was to evaluate the antibacterial activity of 28 plant extracts and oils against four Gram-negative bacterial species. Methods: Experimental, in vitro, evaluation of the activities of 28 plant extracts and oils as well as some antibiotics against E. coli O157:H7, Yersinia enterocolitica O9, Proteus spp., and Klebsiella pneumoniae was performed. The activity against 15 isolates of each bacterium was determined by disc diffusion method at a concentration of 5%. Microdilution susceptibility assay was used in order to determine the minimal inhibitory concentrations (MICs of the plant extracts, oils, and antibiotics. Results: Among the evaluated herbs, only Origanum syriacum L., Thymus syriacus Boiss., Syzygium aromaticum L., Juniperus foetidissima Wild, Allium sativum L., Myristica fragrans Houtt, and Cinnamomum zeylanicum L. essential oils and Laurus nobilis L. plant extract showed anti-bacterial activity. The MIC50 values of these products against the Gram-negative organisms varied from 1.5 (Proteus spp. and K. pneumoniae( and 6.25 µl/ml (Yersinia enterocolitica O9 to 12.5 µl/ml (E. coli O:157. Conclusion: Among the studied essential oils, O. syriacum L., T. syriacus Boiss., C. zeylanicum L., and S. aromaticum L. essential oils were the most effective. Moreover, Cephalosporin and Ciprofloxacin were the most effective antibiotics against almost all the studied bacteria. Therefore, O. syriacum L., T. syriacus Boiss., C. zeylanicum L., and S. aromaticum L. could act as bactericidal agents against Gram-negative bacteria.

Plastome Evolution in the Sole Hemiparasitic Genus Laurel Dodder (Cassytha) and Insights into the Plastid Phylogenomics of Lauraceae.

Science.gov (United States)

Wu, Chung-Shien; Wang, Ting-Jen; Wu, Chia-Wen; Wang, Ya-Nan; Chaw, Shu-Miaw

2017-10-01

To date, little is known about the evolution of plastid genomes (plastomes) in Lauraceae. As one of the top five largest families in tropical forests, the Lauraceae contain many species that are important ecologically and economically. Lauraceous species also provide wonderful materials to study the evolutionary trajectory in response to parasitism because they contain both nonparasitic and parasitic species. This study compared the plastomes of nine Lauraceous species, including the sole hemiparasitic and herbaceous genus Cassytha (laurel dodder; here represented by Cassytha filiformis). We found differential contractions of the canonical inverted repeat (IR), resulting in two IR types present in Lauraceae. These two IR types reinforce Cryptocaryeae and Neocinnamomum-Perseeae-Laureae as two separate clades. Our data reveal several traits unique to Cas. filiformis, including loss of IRs, loss or pseudogenization of 11 ndh and rpl23 genes, richness of repeats, and accelerated rates of nucleotide substitutions in protein-coding genes. Although Cas. filiformis is low in chlorophyll content, our analysis based on dN/dS ratios suggests that both its plastid house-keeping and photosynthetic genes are under strong selective constraints. Hence, we propose that short generation time and herbaceous lifestyle rather than reduced photosynthetic ability drive the accelerated rates of nucleotide substitutions in Cas. filiformis. © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

Laurel Clark Earth Camp: Building a Framework for Teacher and Student Understanding of Earth Systems

Science.gov (United States)

Colodner, D.; Buxner, S.; Schwartz, K.; Orchard, A.; Titcomb, A.; King, B.; Baldridge, A.; Thomas-Hilburn, H.; Crown, D. A.

2013-04-01

Laurel Clark Earth Camp is designed to inspire teachers and students to study their world through field experiences, remote sensing investigations, and hands on exploration, all of which lend context to scientific inquiry. In three different programs (for middle school students, for high school students, and for teachers) participants are challenged to understand Earth processes from the perspectives of both on-the ground inspection and from examination of satellite images, and use those multiple perspectives to determine best practices on both a societal and individual scale. Earth Camp is a field-based program that takes place both in the “natural” and built environment. Middle School Earth Camp introduces students to a variety of environmental science, engineering, technology, and societal approaches to sustainability. High School Earth Camp explores ecology and water resources from southern Arizona to eastern Utah, including a 5 day rafting trip. In both camps, students compare environmental change observed through repeat photography on the ground to changes observed from space. Students are encouraged to utilize their camp experience in considering their future course of study, career objectives, and lifestyle choices. During Earth Camp for Educators, teachers participate in a series of weekend workshops to explore relevant environmental science practices, including water quality testing, biodiversity surveys, water and light audits, and remote sensing. Teachers engage students, both in school and after school, in scientific investigations with this broad based set of tools. Earth Stories from Space is a website that will assist in developing skills and comfort in analyzing change over time and space using remotely sensed images. Through this three-year NASA funded program, participants will appreciate the importance of scale and perspective in understanding Earth systems and become inspired to make choices that protect the environment.

Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichehys nobilis) and silver carp (Hypophthalmichthys molitrix).

Science.gov (United States)

Liu, Haiyang; Pang, Meixia; Yu, Xiaomu; Zhou, Ying; Tong, Jingou; Fu, Beide

2018-01-05

Sex-specific markers are powerful tools for identifying sex-determination system in various animals. Bighead carp (Hypophthalmichehys nobilis) and silver carp (Hypophthalmichthys molitrix) are two of the most important edible fish in Asia, which have a long juvenility period that can lasts for 4-5 years. In this study, we found one sex-specific marker by next-generation sequencing together with bioinformatics analysis in bighead carp. The male-specific markers were used to perform molecular sexing in the progenies of artificial gynogenetic diploids and found all progenies (n = 160) were females. Meanwhile, around 1 : 1 sex ratio was observed in a total of 579 juvenile offspring from three other families. To further extend the male-specific region, we performed genome walking and got a male-specific sequence of 8,661 bp. Five pairs of primers were designed and could be used to efficiently distinguish males from females in bighead carp and silver carp. The development of these male-specific markers and results of their molecular sexing in different populations provide strong evidence for a sex determination system of female homogametry or male heterogametry (XX/XY) in bighead carp and silver carp. To the best of our knowledge, this is the first report of effective sex-specific markers in these two large carp species. © The Author(s) 2018. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

Assessment System for Aircraft Noise (ASAN) Citation Database. Volume 2

Science.gov (United States)

1989-12-01

georgei 01.07.18.03.04.02 * Gambusia heterochir 01.07.18.03.04.04 * Gambusia nobilis 01.07.18.03.04.03 * Gambusia, Amistad 01.07.18.03.04.01 * Gambusia...01.07.18.03.04.03 ..... *Gambusia amistadensis ..... *Gambusia, Amistad 01.07.18.03.04.04 ..... *Gambusia nobilis ..... *Gambusia, Pecos

Potential of contact insecticides to control Xyleborus glabratus (Coleoptera: Curculionidae), a vector of laurel wilt disease in avocados.

Science.gov (United States)

Carrillo, Daniel; Crane, Jonathan H; Peña, Jorge E

2013-12-01

Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae: Scolytinae) is an invasive ambrosia beetle that vectors laurel wilt, a new disease that threatens avocado and other species in the Lauraceae Family. The lethal concentrations (LC50 & 90) of nine commercial insecticides to X. glabratus were determined by using a bolt-dip bioassay. Different formulations of bifenthrin, permethrin, fenpropathrin, z-cypermethrin + bifenthrin, 1-cyhalothrin + thiamethoxam, malathion, chlorpyrifos, carbaryl, and methomyl were tested. Four concentrations of each insecticide were tested (0.5, 0.1, 0.03, and 0.01 of the label rate) and with water as a control. Beetles were exposed to treated bolts and mortality registered 48 h later. After 2 wk, bolts were destructively sampled to determine the number of beetles that constructed galleries and were alive inside the wood. Probit analysis was used to determine the LC50 & 90. Six pesticides were applied directly to the trunk and limbs of avocado trees in a commercial grove. Limbs of treated trees were cut weekly after the application and exposed to X. glabratus to determine the number of beetles boring into the logs. The toxicity of pesticides to X. glabratus was greatly reduced 2 wk after application. Among the tested pesticides, malathion and z-cypermethrin + bifenthrin provided the best suppression of X. glabratus. Among the insecticides registered for use in avocado, fenpropathrin and malathion were the most effective in protecting trees from attack by X. glabratus. Other pesticides that are currently not registered for use in avocados could be useful for managing this ambrosia beetle.

A note on the occurrence of praniza larvae of Gnathiidae (Crustacea, Isopoda on fishes from Northeast of Pará, Brazil

Directory of Open Access Journals (Sweden)

Daniel G. Diniz

2008-12-01

Full Text Available The infection of the estuarine teleost fishes Mugil gaimardianus Desmarest, 1831 (Mugilidae, Arius phrygiatus Valenciennes, 1839 (Ariidae, Conodon nobilis Linnaeus, 1759 (Haemulidae, Cetengraulis edentulus Cuvier, 1829 (Engraulidae, and Anableps anableps Linnaeus, 1758 (Anablepidae by praniza larvae of Gnathiidae (Crustacea, Isopoda was studied in specimens fished off the Atlantic Ocean in Northeast of Pará State, near Bragança, Brazil. The highest infection prevalence value was found in Anableps anableps (42.3% and the lowest in Conodon nobilis (9.1%. The mean intensity varied from 1 parasitein Conodon nobilis to 19.5 in Arius phrygiatus. A description of the larvae is provided. The morphology of the mouthparts is related to the blood sucking activity, and is compared with the characteristics of other gnathiidae species.Foi estudada a parasitose dos peixes estuarinos Mugil gaimardianus Desmarest, 1831 (Mugilidae, Arius phrygiatus Valenciennes, 1839 (Ariidae, Conodon nobilis Linnaeus, 1759 (Haemulidae, Cetengraulis edentulus Cuvier, 1829 (Engraulidae, e Anableps anableps Linnaeus, 1758 (Anablepidae por larvas praniza de Gnathiidae (Crustacea, Isopoda em exemplares pescados no Oceano Atlântico, no Nordeste do Pará, próximo a Bragança, Brasil. O valor mais elevado da prevalência da infecção foi observado em A. anableps (42,3% e o menor em C. nobilis (9,1%. A intensidade média da parasitose variou entre 1 parasita em C. nobilis até 19,5 em A. phrygiatus. Efetua-se a descrição da larva, verificando-se que a morfologia da armadura bucal está relacionada com a atividade sugadora de sangue, e faz-se a comparação com as características de outras espécies.

Differences in health symptoms among residents living near illegal dump sites in Los Laureles Canyon, Tijuana, Mexico: a cross sectional survey.

Science.gov (United States)

Al-Delaimy, Wael K; Larsen, Catherine Wood; Pezzoli, Keith

2014-09-15

Living near landfills is a known health hazard prompting recognition of environmental injustice. The study aim was to compare self-reported symptoms of ill health among residents of four neighborhoods, living in haphazardly constructed settlements surrounded by illegal dumpsites in Tijuana, Mexico. One adult from each of 388 households located in Los Laureles Canyon were interviewed about demographics, health status, and symptoms. Distance from each residence to both the nearest dumpsite and the canyon bottom was assessed. The neighborhoods were selected from locations within the canyon, and varied with respect to proximity to dump sites. Residents of San Bernardo reported significantly higher frequencies of ill-health symptoms than the other neighborhoods, including extreme fatigue (OR 3.01 (95% CI 1.6-5.5)), skin problems/irritations (OR 2.73 (95% CI 1.3-5.9)), stomach discomfort (OR 2.47 (1.3-4.8)), eye irritation/tears (OR 2.02 (1.2-3.6)), and confusion/difficulty concentrating (OR 2.39 (1.2-4.8)). Proximity to dumpsites did not explain these results, that varied only slightly when adjusted for distance to nearest dumpsite or distance to the canyon bottom. Because San Bernardo has no paved roads, we hypothesize that dust and the toxicants it carries is a possible explanation for this difference. Studies are needed to further document this association and sources of toxicants.

Effects of Chilling and Partial Freezing on Rigor Mortis Changes of Bighead Carp (Aristichthys nobilis) Fillets: Cathepsin Activity, Protein Degradation and Microstructure of Myofibrils.

Science.gov (United States)

Lu, Han; Liu, Xiaochang; Zhang, Yuemei; Wang, Hang; Luo, Yongkang

2015-12-01

To investigate the effects of chilling and partial freezing on rigor mortis changes in bighead carp (Aristichthys nobilis), pH, cathepsin B, cathepsin B+L activities, SDS-PAGE of sarcoplasmic and myofibrillar proteins, texture, and changes in microstructure of fillets at 4 °C and -3 °C were determined at 0, 2, 4, 8, 12, 24, 48, and 72 h after slaughter. The results indicated that pH of fillets (6.50 to 6.80) was appropriate for cathepsin function during the rigor mortis. For fillets that were chilled and partially frozen, the cathepsin activity in lysosome increased consistently during the first 12 h, followed by a decrease from the 12 to 24 h, which paralleled an increase in activity in heavy mitochondria, myofibrils and sarcoplasm. There was no significant difference in cathepsin activity in lysosomes between fillets at 4 °C and -3 °C (P > 0.05). Partially frozen fillets had greater cathepsin activity in heavy mitochondria than chilled samples from the 48 to 72 h. In addition, partially frozen fillets showed higher cathepsin activity in sarcoplasm and lower cathepsin activity in myofibrils compared with chilled fillets. Correspondingly, we observed degradation of α-actinin (105 kDa) by cathepsin L in chilled fillets and degradation of creatine kinase (41 kDa) by cathepsin B in partially frozen fillets during the rigor mortis. The decline of hardness for both fillets might be attributed to the accumulation of cathepsin in myofibrils from the 8 to 24 h. The lower cathepsin activity in myofibrils for fillets that were partially frozen might induce a more intact cytoskeletal structure than fillets that were chilled. © 2015 Institute of Food Technologists®

Allelopathic Effect of Essential Oil of Sweet Bay (Laurus nobilis L. on Germination and Seedling Vigor of Velvetleaf (Abutilon theopharasti L. and Field Bindweed (Convolvulus arvensis L.

Directory of Open Access Journals (Sweden)

Bahram Mirshekari

2016-06-01

Full Text Available To study allelopatic effect of sweet bay essence concentrations (0, 100, 200, 300 and 400 ppm on germination and early establishment of velvetleaf and field bindweed an experiment was conducted at Islamic Azad University of Tabriz, Iran, during 2013. Results indicated that germination percentage of non-treated seeds was 73.3%, and that of treated seeds 64.7%. Plant height at 400 ppm concentration was shorter than other treatments. Mean leaf area per plant of weeds ranged from 13.5 cm2 in control up to 9.7 cm2 in 300 ppm and 400 ppm concertrations. Dry weight per weed plant of the seeds treated with 300 and 400 ppm concentrations was twice lower than of untreated seeds. Vigor index of seedling from seeds treated with 100 and 200 ppm essence and control were 1.5, 1.5 and 2.6 times higher than those treated with 300-400 ppm, respectively. Regression analysis showed that germination percentage, leaf area and dry weight per plant did have higher effect on seedling vigor index. It can be concluded that essential oil of sweet-bay may have potential in controlling weeds, especially in the higher concentrations. Therefor, it could be used in the synthesis of bioherbicides compounds to control weeds.

Isolations from the redbay ambrosia beetle, Xyleborus glabratus, confirm that the laurel wilt pathogen, Raffaelea lauricola, originated in Asia.

Science.gov (United States)

Harrington, Thomas C; Yun, Hye Young; Lu, Sheng-Shan; Goto, Hideaki; Aghayeva, Dilzara N; Fraedrich, Stephen W

2011-01-01

The laurel wilt pathogen Raffaelea lauricola was hypothesized to have been introduced to the southeastern USA in the mycangium of the redbay ambrosia beetle, Xyleborus glabratus, which is native to Asia. To test this hypothesis adult X. glabratus were trapped in Taiwan and on Kyushu Island, Japan, in 2009, and dead beetles were sent to USA for isolation of fungal symbionts. Individual X. glabratus were macerated in glass tissue grinders, and the slurry was serially diluted and plated onto malt agar medium amended with cycloheximide, a medium semiselective for Ophiostoma species and their anamorphs, including members of Raffaelea. R. lauricola was isolated from 56 of 85 beetles in Taiwan and 10 of 16 beetles in Japan at up to an estimated 10 000 CFUs per beetle. The next most commonly isolated species was R. ellipticospora, which also has been recovered from X. glabratus trapped in the USA, as were two other fungi isolated from beetles in Taiwan, R. fusca and R. subfusca. Three unidentified Raffaelea spp. and three unidentified Ophiostoma spp. were isolated rarely from X. glabratus collected in Taiwan. Isolations from beetles similarly trapped in Georgia, USA, yielded R. lauricola and R. ellipticospora in numbers similar to those from beetles trapped in Taiwan and Japan. The results support the hypothesis that R. lauricola was introduced into the USA in mycangia of X. glabratus shipped to USA in solid wood packing material from Asia. However differences in the mycangial mycoflora of X. glabratus in Taiwan, Japan and USA suggest that the X. glabratus population established in USA originated in another part of Asia.

Partnerships Between Ambrosia Beetles and Fungi: Lineage-Specific Promiscuity Among Vectors of the Laurel Wilt Pathogen, Raffaelea lauricola.

Science.gov (United States)

Saucedo-Carabez, J R; Ploetz, Randy C; Konkol, J L; Carrillo, D; Gazis, R

2018-04-20

Nutritional mutualisms that ambrosia beetles have with fungi are poorly understood. Although these interactions were initially thought to be specific associations with a primary symbiont, there is increasing evidence that some of these fungi are associated with, and move among, multiple beetle partners. We examined culturable fungi recovered from mycangia of ambrosia beetles associated with trees of Persea humilis (silk bay, one site) and P. americana (avocado, six commercial orchards) that were affected by laurel wilt, an invasive disease caused by a symbiont, Raffaelea lauricola, of an Asian ambrosia beetle, Xyleborus glabratus. Fungi were isolated from 20 adult females of X. glabratus from silk bay and 70 each of Xyleborus affinis, Xyleborus bispinatus, Xyleborus volvulus, Xyleborinus saxesenii, and Xylosandrus crassiusculus from avocado. With partial sequences of ribosomal (LSU and SSU) and nuclear (β-tubulin) genes, one to several operational taxonomic units (OTUs) of fungi were identified in assayed individuals. Distinct populations of fungi were recovered from each of the examined beetle species. Raffaelea lauricola was present in all beetles except X. saxesenii and X. crassiusculus, and Raffaelea spp. predominated in Xyleborus spp. Raffaelea arxii, R. subalba, and R. subfusca were present in more than a single species of Xyleborus, and R. arxii was the most abundant symbiont in both X. affinis and X. volvulus. Raffaelea aguacate was detected for the first time in an ambrosia beetle (X. bispinatus). Yeasts (Ascomycota, Saccharomycotina) were found consistently in the mycangia of the examined beetles, and distinct, putatively co-adapted populations of these fungi were associated with each beetle species. Greater understandings are needed for how mycangia in ambrosia beetles interact with fungi, including yeasts which play currently underresearched roles in these insects.

Diploid hybrid origin of Ostryopsis intermedia (Betulaceae) in the Qinghai-Tibet Plateau triggered by Quaternary climate change.

Science.gov (United States)

Liu, Bingbing; Abbott, Richard J; Lu, Zhiqiang; Tian, Bin; Liu, Jianquan

2014-06-01

Despite the well-known effects that Quaternary climate oscillations had on shaping intraspecific diversity, their role in driving homoploid hybrid speciation is less clear. Here, we examine their importance in the putative homoploid hybrid origin and evolution of Ostryopsis intermedia, a diploid species occurring in the Qinghai-Tibet Plateau (QTP), a biodiversity hotspot. We investigated interspecific relationships between this species and its only other congeners, O. davidiana and O. nobilis, based on four sets of nuclear and chloroplast population genetic data and tested alternative speciation hypotheses. All nuclear data distinguished the three species clearly and supported a close relationship between O. intermedia and the disjunctly distributed O. davidiana. Chloroplast DNA sequence variation identified two tentative lineages, which distinguished O. intermedia from O. davidiana; however, both were present in O. nobilis. Admixture analyses of genetic polymorphisms at 20 SSR loci and sequence variation at 11 nuclear loci and approximate Bayesian computation (ABC) tests supported the hypothesis that O. intermedia originated by homoploid hybrid speciation from O. davidiana and O. nobilis. We further estimated that O. davidiana and O. nobilis diverged 6-11 Ma, while O. intermedia originated 0.5-1.2 Ma when O. davidiana is believed to have migrated southward, contacted and hybridized with O. nobilis possibly during the largest Quaternary glaciation that occurred in this region. Our findings highlight the importance of Quaternary climate change in the QTP in causing hybrid speciation in this important biodiversity hotspot. © 2014 John Wiley & Sons Ltd.

Differences in Health Symptoms among Residents Living Near Illegal Dump Sites in Los Laureles Canyon, Tijuana, Mexico: A Cross Sectional Survey

Directory of Open Access Journals (Sweden)

Wael K. Al-Delaimy

2014-09-01

Full Text Available Living near landfills is a known health hazard prompting recognition of environmental injustice. The study aim was to compare self-reported symptoms of ill health among residents of four neighborhoods, living in haphazardly constructed settlements surrounded by illegal dumpsites in Tijuana, Mexico. One adult from each of 388 households located in Los Laureles Canyon were interviewed about demographics, health status, and symptoms. Distance from each residence to both the nearest dumpsite and the canyon bottom was assessed. The neighborhoods were selected from locations within the canyon, and varied with respect to proximity to dump sites. Residents of San Bernardo reported significantly higher frequencies of ill-health symptoms than the other neighborhoods, including extreme fatigue (OR 3.01 (95% CI 1.6–5.5, skin problems/irritations (OR 2.73 (95% CI 1.3–5.9, stomach discomfort (OR 2.47 (1.3–4.8, eye irritation/tears (OR 2.02 (1.2–3.6, and confusion/difficulty concentrating (OR 2.39 (1.2–4.8. Proximity to dumpsites did not explain these results, that varied only slightly when adjusted for distance to nearest dumpsite or distance to the canyon bottom. Because San Bernardo has no paved roads, we hypothesize that dust and the toxicants it carries is a possible explanation for this difference. Studies are needed to further document this association and sources of toxicants.

Differences in Health Symptoms among Residents Living Near Illegal Dump Sites in Los Laureles Canyon, Tijuana, Mexico: A Cross Sectional Survey

Science.gov (United States)

Al-Delaimy, Wael K.; Wood Larsen, Catherine; Pezzoli, Keith

2014-01-01

Living near landfills is a known health hazard prompting recognition of environmental injustice. The study aim was to compare self-reported symptoms of ill health among residents of four neighborhoods, living in haphazardly constructed settlements surrounded by illegal dumpsites in Tijuana, Mexico. One adult from each of 388 households located in Los Laureles Canyon were interviewed about demographics, health status, and symptoms. Distance from each residence to both the nearest dumpsite and the canyon bottom was assessed. The neighborhoods were selected from locations within the canyon, and varied with respect to proximity to dump sites. Residents of San Bernardo reported significantly higher frequencies of ill-health symptoms than the other neighborhoods, including extreme fatigue (OR 3.01 (95% CI 1.6–5.5)), skin problems/irritations (OR 2.73 (95% CI 1.3–5.9)), stomach discomfort (OR 2.47 (1.3–4.8)), eye irritation/tears (OR 2.02 (1.2–3.6)), and confusion/difficulty concentrating (OR 2.39 (1.2–4.8)). Proximity to dumpsites did not explain these results, that varied only slightly when adjusted for distance to nearest dumpsite or distance to the canyon bottom. Because San Bernardo has no paved roads, we hypothesize that dust and the toxicants it carries is a possible explanation for this difference. Studies are needed to further document this association and sources of toxicants. PMID:25226411

7 CFR 301.92-2 - Restricted, regulated, and associated articles; lists of proven hosts and associated plant taxa.

Science.gov (United States)

2010-01-01

... virginiana Witch hazel *Heteromeles arbutifolia Toyon *Kalmia spp. Kalmia—includes all species, hybrids, and... tree Pittosporum undulatum Victorian box Prunus laurocerasus English laurel, cherry laurel Prunus...

The influence of chicken eggshell powder as a buffer on biohydrogen production from rotten orange (Citrus nobilis var. microcarpa) with immobilized mixed culture

Science.gov (United States)

Damayanti, Astrilia; Sarto, Syamsiah, Siti; Sediawan, Wahyudi B.

2017-06-01

This research observed the influence of chicken eggshell on hydrogen production from anaerobic fermentation of rotten orange (Citrus nobilis var. microcarpa) using batch method at 36 °C and pH 7. Fermentation material were varied in several types, the first type was meat and peel of oranges with VS of 59.152 g.L-1 in A, B, C, and D compositions. The second type was orange meat added with peel (OMP) with VS of 36.852 g.L-1. The immobilized ingredients used in the experiment consisted of 2 % (w/v) alginate and active carbon with the ratio of 1:1. 3.2 g chicken eggshell powder was added to the first type of material (substrates A, B, C, and D). Results showed that pH during fermentation process using chicken eggshell as a buffer was constant at 5.5; however, without the use of chicken eggshell, the pH decreased to 3.8 and increased slightly before it stayed stable at 4.0. The total amount of gas produced in sample using the chicken eggshell was 46,35 mL.mg VS-1 and in sample produced without the eggshell, it was 3,4 mL.mg VS-1. The production of hydrogen in substrate that used chicken eggshell was 1,276 mL.gVS-1 in average on the first day. Meanwhile, for the substrate with no addition of chicken eggshell, the average production of hydrogen was 0,163 mL.gVS-1. The reduction of volatile solid (VS) in sample that used chicken eggshell was 24 %, while in sample produced without addition of chicken eggshell, the reduction was 12 %. The liquid compounds (VFA) produced in the fermentation using chicken eggshell were acetic acid and butyric acid. Meanwhile, without addition of chicken eggshell, the products were acetic acid, butyric acid, and propionic acid. This study shows that addition of chicken eggshell as a buffer effectively contributed to hydrogen production during fermentation of rotten oranges.

Vertical Distribution and Daily Flight Periodicity of Ambrosia Beetles (Coleoptera: Curculionidae) in Florida Avocado Orchards Affected by Laurel Wilt.

Science.gov (United States)

Menocal, Octavio; Kendra, Paul E; Montgomery, Wayne S; Crane, Jonathan H; Carrillo, Daniel

2018-03-08

Ambrosia beetles have emerged as significant pests of avocado ((Persea americana Mill. [Laurales: Lauraceae])) due to their association with pathogenic fungal symbionts, most notably Raffaelea lauricola T.C. Harr., Fraedrich & Aghayeva (Ophiostomatales: Ophiostomataceae), the causal agent of the laurel wilt (LW) disease. We evaluated the interaction of ambrosia beetles with host avocado trees by documenting their flight height and daily flight periodicity in Florida orchards with LW. Flight height was assessed passively in three avocado orchards by using ladder-like arrays of unbaited sticky traps arranged at three levels (low: 0-2 m; middle: 2-4 m; high: 4-6 m). In total, 1,306 individuals of 12 Scolytinae species were intercepted, but six accounted for ~95% of the captures: Xyleborus volvulus (Fabricius) (Coleoptera: Curculionidae), Xyleborinus saxesenii Ratzeburg (Coleoptera: Curculionidae), Euplatypus parallelus (Fabricius) (Coleoptera: Curculionidae), Xyleborus bispinatus Eichhoff (Coleoptera: Curculionidae), Xyleborus affinis Eichhoff (Coleoptera: Curculionidae), and Hypothenemus sp. (Coleoptera: Curculionidae). The primary vector of R. lauricola, Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae), was not detected. Females of X. volvulus showed a preference for flight at low levels and X. bispinatus for the low and middle levels; however, captures of all other species were comparable at all heights. At a fourth orchard, a baiting method was used to document flight periodicity. Females of X. saxesenii and Hypothenemus sp. were observed in flight 2-2.5 h prior to sunset; X. bispinatus, X. volvulus, and X. affinis initiated flight at ~1 h before sunset and Xylosandrus crassiusculus (Motschulsky) (Coleoptera: Curculionidae) at 30 min prior to sunset. Results suggest that ambrosia beetles in South Florida fly near sunset (when light intensity and wind speed decrease) at much greater heights than previously assumed and have species-specific patterns in host

On Amherstia nobilis

Indian Academy of Sciences (India)

I met Dr Bappu for the first time in the early days of the Kavalur telescope in the ... Bappugot very interested and the next exercise was to get the know-how on ... gardeners had clear instructions to let in a little sunlight every morning and then ...

Cytotoxicity of South-African medicinal plants towards sensitive and multidrug-resistant cancer cells.

Science.gov (United States)

Saeed, Mohamed E M; Meyer, Marion; Hussein, Ahmed; Efferth, Thomas

2016-06-20

Traditional medicine plays a major role for primary health care worldwide. Cancer belongs to the leading disease burden in industrialized and developing countries. Successful cancer therapy is hampered by the development of resistance towards established anticancer drugs. In the present study, we investigated the cytotoxicity of 29 extracts from 26 medicinal plants of South-Africa against leukemia cell lines, most of which are used traditionally to treat cancer and related symptoms. We have investigated the plant extracts for their cytotoxic activity towards drug-sensitive parental CCRF-CEM leukemia cells and their multidrug-resistant P-glycoprotein-overexpressing subline, CEM/ADR5000 by means of the resazurin assay. A panel of 60 NCI tumor cell lines have been investigated for correlations between selected phytochemicals from medicinal plants and the expression of resistance-conferring genes (ABC-transporters, oncogenes, tumor suppressor genes). Seven extracts inhibited both cell lines (Acokanthera oppositifolia, Hypoestes aristata, Laurus nobilis, Leonotis leonurus, Plectranthus barbatus, Plectranthus ciliates, Salvia apiana). CEM/ADR5000 cells exhibited a low degree of cross-resistance (3.35-fold) towards the L. leonurus extract, while no cross-resistance was observed to other plant extracts, although CEM/ADR5000 cells were highly resistant to clinically established drugs. The log10IC50 values for two out of 14 selected phytochemicals from these plants (acovenoside A and ouabain) of 60 tumor cell lines were correlated to the expression of ABC-transporters (ABCB1, ABCB5, ABCC1, ABCG2), oncogenes (EGFR, RAS) and tumor suppressors (TP53). Sensitivity or resistance of the cell lines were not statistically associated with the expression of these genes, indicating that multidrug-resistant, refractory tumors expressing these genes may still respond to acovenoside A and ouabain. The bioactivity of South African medicinal plants may represent a basis for the development

Some Physical, Chemical, and Biological Parameters of Samples of Scleractinium Coral Aquaculture Skeleton Used for Reconstruction/Engineering of the Bone Tissue.

Science.gov (United States)

Popov, A A; Sergeeva, N S; Britaev, T A; Komlev, V S; Sviridova, I K; Kirsanova, V A; Akhmedova, S A; Dgebuadze, P Yu; Teterina, A Yu; Kuvshinova, E A; Schanskii, Ya D

2015-08-01

Physical and chemical (phase and chemical composition, dynamics of resorption, and strength properties), and biological (cytological compatibility and scaffold properties of the surface) properties of samples of scleractinium coral skeletons from aquacultures of three types and corresponding samples of natural coral skeletons (Pocillopora verrucosa, Acropora formosa, and Acropora nobilis) were studied. Samples of scleractinium coral aquaculture skeleton of A. nobilis, A. formosa, and P. verrucosa met the requirements (all study parameters) to materials for osteoplasty and 3D-scaffolds for engineering of bone tissue.

Interaction between rancidity and organoleptic parameters of anchovy marinade (Engraulis encrasicolus L. 1758) include essential oils.

Science.gov (United States)

Turan, Hülya; Kocatepe, Demet; Keskin, İrfan; Altan, Can Okan; Köstekli, Bayram; Candan, Canan; Ceylan, Asuman

2017-09-01

This study was carried out to evaluate the lipid oxidation and sensory attributes of anchovy marinated with 10% NaCl+4% alcohol vinegar+0.2% citric acid solution and 0.1% different essential oils. Group A Control: only sunflower seed oil, Group B: sunflower seed oil+0.1% rosemary oil, Group C: sunflower seed oil+0.1% coriander oil, Group D: sunflower seed oil+0.1% laurel oil and Group E: sunflower seed oil+0.1% garlic oil. During storage, lipid oxidation as indicated by the 2-thiobarbituric acid reactive substances (TBARs) values of the control group were significantly higher than the other groups containing essential oils. The results showed that the essential oils have retarding effect on lipids oxidation. This effect was the highest in laurel oil during initial 3 months; and it was similar to laurel oil and rosemary oil in the fourth month; in all the essential oil added groups in 6 month. L*(brightness) values were similar for all groups in first fourth months but, at the last 2 months, group using laurel oil was found better. Yellowness (b*) was similar in all groups during the intial 3 months whereas, after that lower values in the groups that used laurel and rosemary oils were detected. The study concluded that marination with 0.1% laurel oil of anchovy can retard lipid oxidation and improve the sensory attributes of the product during refrigerated storage.

Antithrombin activity of medicinal plants of the Azores.

Science.gov (United States)

de Medeiros, J M; Macedo, M; Contancia, J P; Nguyen, C; Cunningham, G; Miles, D H

2000-09-01

A chromogenic bioassay was utilized to determine the antithrombin activity of methylene chloride and methanol extracts prepared from 50 plants of Azores. Extracts of the six plants Hedychium gardneranum, Tropaeolum majus, Gunnera tinctoria, Hedera helix, Festuca jubata and Laurus azorica demonstrated activity of 78% or higher in this bioassay system.

Toxic effects of chromic sulphate on the common carp, Cyprinus carpio

Energy Technology Data Exchange (ETDEWEB)

Wong, M.H.; Lau, W.M.; Tong, T.Y.; Liu, W.K.; Luk, K.C.

1982-02-01

The effects of chromic sulphate, which is commonly used in the tanning of leather, on the common carp, Cyprinus carpio and the bighead, Aristichthys nobilis, were studied. C. carpio was found to be more susceptible than A. nobilis to chromic sulphate at the lowest concentration tested (100 mg/l) whereas results obtained at other concentration were similar for both species. Damage to the gills, liver and intestine was observed by histopathological examination. A wide range of chemicals is used in the different stages of treating leather and a possible treatment of the effluent from the tanneries is suggested.

Genome Sizes in Hepatica Mill: (Ranunculaceae Show a Loss of DNA, Not a Gain, in Polyploids

Directory of Open Access Journals (Sweden)

B. J. M. Zonneveld

2010-01-01

, and a possible pentaploid. The somatic nuclear DNA contents (2C-value, as measured by flow cytometry with propidium iodide, were shown to range from 33 to 80 pg. The Asiatic and American species, often considered subspecies of H. nobilis, could be clearly distinguished from European H. nobilis. DNA content confirmed the close relationships in the Asiatic species, and these are here considered as subspecies of H. asiatica. Parents for the allotetraploid species could be suggested based on their nuclear DNA content. Contrary to the increase in genome size suggested earlier for Hepatica, a significant (6%–14% loss of nuclear DNA in the natural allopolyploids was found.

7 CFR 301.32-2 - Regulated articles.

Science.gov (United States)

2010-01-01

... jambhiri Lemon, Rough Mediterranean. Citrus latifolia Lime, Persian Oriental. Citrus limon Lemon..., Oriental. Citrus reticulata x C. sinensis = Citrus nobilis Orange, king Mediterranean, Melon, Oriental...

Mitochondrial genome sequencing and development of genetic markers for the detection of DNA of invasive bighead and silver carp (Hypophthalmichthys nobilis and H. molitrix in environmental water samples from the United States.

Directory of Open Access Journals (Sweden)

Heather L Farrington

Full Text Available Invasive Asian bighead and silver carp (Hypophthalmichthys nobilis and H. molitrix pose a substantial threat to North American aquatic ecosystems. Recently, environmental DNA (eDNA, genetic material shed by organisms into their environment that can be detected by non-invasive sampling strategies and genetic assays, has gained recognition as a tool for tracking the invasion front of these species toward the Great Lakes. The goal of this study was to develop new species-specific conventional PCR (cPCR and quantitative (qPCR markers for detection of these species in North American surface waters. We first generated complete mitochondrial genome sequences from 33 bighead and 29 silver carp individuals collected throughout their introduced range. These sequences were aligned with those from other common and closely related fish species from the Illinois River watershed to identify and design new species-specific markers for the detection of bighead and silver carp DNA in environmental water samples. We then tested these genetic markers in the laboratory for species-specificity and sensitivity. Newly developed markers performed well in field trials, did not have any false positive detections, and many markers had much higher detection rates and sensitivity compared to the markers currently used in eDNA surveillance programs. We also explored the use of multiple genetic markers to determine whether it would improve detection rates, results of which showed that using multiple highly sensitive markers should maximize detection rates in environmental samples. The new markers developed in this study greatly expand the number of species-specific genetic markers available to track the invasion front of bighead and silver carp and will improve the resolution of these assays. Additionally, the use of the qPCR markers developed in this study may reduce sample processing time and cost of eDNA monitoring for these species.

Experimental insights into angiosperm origins.

Science.gov (United States)

Lomax, Barry; Lee, Alex; Smilie, Ian; Knight, Charles; Upchurch, Garland

2017-04-01

The angiosperms occupy almost every habitat type on Earth and comprise nearly 90% of extant plant species. Yet this ascendency is a relatively recent (geological) phenomenon. Palaeobotanical evidence indicates a likely first occurrence in the Early Cretaceous followed by a relatively rapid increase in diversity with their rise to dominance marking the onset of modern world. Understanding this diversification event has been a key research question since Darwin commented on this "abominable mystery", and it remains one of the most significant unanswered questions in plant biology. Sequencing work shows that the diversification and radiation was accompanied by successive whole genome duplication (WGD) events. Furthermore proxy data and predictions from long-term carbon cycle models indicate that the angiosperm diversification was accompanied by a decline in atmospheric CO2. These observation raise the intriguing possibility that declining atmospheric CO2 concentration and capacity to undergo polyploidy could have given angiosperms a competitive advantage when compared to other plant groups. Using comparative ecophysiology we set out to test the effects of declining atmospheric CO2 by growing a six species (Ranunculus acris and Polypodium vulgare, chosen to represent Cretaceous understorey angiosperms and pteridophytes respectively. Liquidambar styraciflua and Laurus nobilis represented canopy angiosperms and Ginkgo biloba and Metasequoia glyptostroboides canopy gymnosperms) in controlled conditions across a CO2 gradient (2000, 1200, 800 and 400 ppm) to simulate Cretaceous CO2decline. To test for WGDs we use the relationship between guard cell size and genome size to reconstruct angiosperm genome size as they radiated. Analysis of our fossil dataset shows that earliest angiosperms had a small genome size. Our experimental work shows that angiosperms have a greater capacity for acclimation suggesting that declining CO2 could have acted as a trigger for the angiosperm

Assessing urban forest effects and values, Los Angeles' urban forest

Science.gov (United States)

David J. Nowak; Robert E. III Hoehn; Daniel E. Crane; Lorraine Weller; Antonio. Davila

2011-01-01

An analysis of trees in Los Angeles, CA, reveals that this area has about 6 million trees with tree and shrub canopies that cover 24.9 percent of the city. The most common tree species are Italian cypress, scrub oak, laurel sumac, Mexican fan palm, and Indian laurel, Trees in Los Angeles currently store about 1.3 million tons of carbon (4.7 million tons CO2...

Environmental Exposures, Genetic Polymorphisms and p53 Mutational Spectra in a Case-Control Study of Breast Cancer

Science.gov (United States)

1999-01-01

activities in alimentary tract. Gastroenterology 1997;112:766-75. 38. Moreno A, Pares A, Ortiz J, Enriquez J, Pares X. Alcohol dehydrogenase from human...preliminary data analysis, Dr. Terri Lehman and Bioserve Biotechnologies (Laurel, MD) for their technical expertise, Drs. Joel Gelernter, David Comings...genes with the tyrosine hydroxylase was performed at Bioserve cigarette smoking. In a recent study of the polymorphic Biotechnologies (Laurel, MD, USA

A Picture Guide to Trees of the Gamboa Area, Republic of Panama.

Science.gov (United States)

1985-10-01

THEIR LEAVES Plants that Lose Their Leaves In Wet Season In Dry Season Cordia aliodora (Laurel) Annona spraguei (Cherimoya) Ochroma pyramidale (Balsa...Zanthoxylum species. 77 Cordia aliodora Laurel Boraginaceae Leaves The leaves (13 by 6 cm) are simple, clustered in whorls, pointed at both ends, and...flowers (5mm) lk ri 6m Figure 41. Cordia aliodora. 79 Bursera simarouba Gumbo Limbo Burseraceae I Leaves The leaves are alternate and pinnately compound

In vitro flowering in embryogenic cultures of Kinnow mandarin ...

African Journals Online (AJOL)

AJB SERVER

2006-08-17

Aug 17, 2006 ... Embryogenic cultures of Kinnow mandarin (C. nobilis Lour × C. deliciosa Tenora) were raised from unfertilized ovules ... development of efficient plant tissue culture procedures for in vitro ..... epiphytic orchid. Plant Physiol.

North American Lauraceae: Terpenoid Emissions, Relative Attraction and Boring Preferences of Redbay Ambrosia Beetle, Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae)

OpenAIRE

Kendra, Paul E.; Montgomery, Wayne S.; Niogret, Jerome; Pruett, Grechen E.; Mayfield, Albert E.; MacKenzie, Martin; Deyrup, Mark A.; Bauchan, Gary R.; Ploetz, Randy C.; Epsky, Nancy D.

2014-01-01

The invasive redbay ambrosia beetle, Xyleborus glabratus, is the primary vector of Raffaelea lauricola, a symbiotic fungus and the etiologic agent of laurel wilt. This lethal disease has caused severe mortality of redbay (Persea borbonia) and swampbay (P. palustris) trees in the southeastern USA, threatens avocado (P. americana) production in Florida, and has potential to impact additional New World species. To date, all North American hosts of X. glabratus and suscepts of laurel wilt are mem...

leaves extract on mild steel in acid

African Journals Online (AJOL)

ADOWIE PERE

The volume of the cathodic hydrogen gas evolved was also plotted as a .... prevent the escape of hydrogen gas. The volume .... Clivia nobilis leaves extract on the flow of current ... behaviour of ethanol extract of Piper guinensis as a green ...

Using optimized random amplified polymorphic DNA (RAPD ...

African Journals Online (AJOL)

Administrator

2011-10-19

Oct 19, 2011 ... It is conjectured that its origin is from a cross between tangerine and orange; however, there is ... to better understand the genetic relationship between C. nobilis Lour. Gonggan and other Citrus species; and (2) to address the.

In vitro antifungal activities of 26 plant extracts on mycelial growth of ...

African Journals Online (AJOL)

Antifungal activities of 26 plant extracts were tested against Phytophthora infestans using radial growth technique. While all tested plant extracts produced some antifungal activities Xanthium strumarium, Lauris nobilis, Salvia officinalis and Styrax officinalis were the most active plants that showed potent antifungal activity.

Tools for assessing kinship, population structure, phylogeography, and interspecific hybridization in Asian carps invasive to the Mississippi River, USA: isolation and characterization of novel tetranucleotide microsatellite DNA loci in silver carp Hypophthalmichthys molitrix

Science.gov (United States)

King, T.L.; Eackles, M.S.; Chapman, D.C.

2011-01-01

We document the isolation and characterization of novel tetranucleotide microsatellite DNA markers for the invasive silver carp Hypophthalmichthys molitrix and provide the results of cross-species amplification for three additional invasive carp species: bighead (H. nobilis), grass (Ctenopharyngodon idella) and black (Mylopharyngodon piceus). In the target species these markers yielded levels of allelic diversity (average 4.4 alleles/locus) and heterozygosity (average 54.7%) sufficient to: (1) provide unique multilocus genotypes; (2) delineate kinship relationships; (3) differentiate populations/species; (4) estimate effective population sizes; and (5) provide unique demographic perspectives for control or eradication. Currently these markers are being utilized to determine the degree of introgressive hybridization between H. molitrix and H. nobilis, to quantify gene flow between different sub-basins established in the central United States, and to assess the demographic status of sub-basin groups. This information will be critically important in the management/control of these invasive species.

2017: Alterum Annum Nobilis Arthroscopy (AANA).

Science.gov (United States)

Lubowitz, James H; Brand, Jefferson C; Rossi, Michael J

2017-12-01

In 2017, the Arthroscopy Association of North America (AANA) continues to be defined. Arthroscopy is an international journal and represents a worldwide Academic Association of Naturally-gifted Arthroscopists (AANA). When we look to the past to better understand the present and to foretell the future, we observe that our cooperative effort, merging surgeons and scientists, researchers and scholars, and authors and readers, has evolved beyond national borders. Our endeavor is an intercontinental, All-embracing Avant-garde of Notable Academics (AANA). In summary, Arthroscopy, like AANA, is global. Copyright © 2017 Arthroscopy Association of North America. Published by Elsevier Inc. All rights reserved.

Assessment of allelopathic potential of selected medicinal plants of ...

African Journals Online (AJOL)

Inula falconeri, Inula koelzii, Lactuca dissecta and Anthemis nobilis were collected from Himalaya and Hindukush ranges of Pakistan and their allelopathic effect was studied through Sandwich and Homogenated Sandwich methods. The study also aimed at analyzing whether method and concentration can affect the overall ...

IIn vitro antifungal evaluation of various plant extracts against early ...

African Journals Online (AJOL)

Antifungal activities of 27 plant extracts were tested against Alternaria solani (E. & M.) Jones and Grout using radial growth technique. While all tested plant extracts produced some antifungal activities, the results revealed that Circium arvense, Humulus lupulus, Lauris nobilis and Salvia officinalis showed significant ...

The effect of feeding time on dispersal of Virola seeds by toucans determined from GPS tracking and accelerometers

NARCIS (Netherlands)

Kays, Roland; Jansen, Patrick A.; Knecht, Elise M. H.; Vohwinkel, Reinhard; Wikelski, Martin

2011-01-01

Seed dispersal is critical to understanding forest dynamics but is hard to study because tracking seeds is difficult. Even for the best-studied dispersal system of the Neotropics, Virola nobilis, the dispersal kernel remains unknown. We combined high-resolution GPS/3D-acceleration bird tracking,

Traditional Mediterranean plants: characterization and use of an essential oils mixture to treat Malassezia otitis externa in atopic dogs.

Science.gov (United States)

Nardoni, Simona; Pistelli, Luisa; Baronti, Ilenia; Najar, Basma; Pisseri, Francesca; Bandeira Reidel, Rose Vanessa; Papini, Roberto; Perrucci, Stefania; Mancianti, Francesca

2017-08-01

Several plants extracts from Mediterranean countries are traditionally employed in skin troubles both in humans and in animals. Malassezia pachydermatis is a lipophylic yeast responsible for otitis externa and dermatitis in dogs and for cutaneous and systemic disease in humans. Five mixtures of essential oils obtained from Mediterranean plants (Citrus paradisi, Salvia sclarea, Ocimum basilicum, Rosmarinus officinalis, Citrus limon, Anthemis nobilis, Lavandula hybrida and Thymus vulgaris) provided with antifungal and/or anti-inflammatory action assayed in vitro, were tested in vivo versus M. pachydermatis to treat once daily for 2 weeks 25 atopic dogs with Malassezia otitis externa. Mixture composed by C. limon 1%, S. sclarea 0,5%, R. officinalis 1%, A. nobilis 0,5% yielded excellent results in all treated dogs. Despite of clinical resolution after all treatments the number of blastospores did not decrease. This study confirms recent findings suggesting a multifactorial alternative approach for the management of canine Malassezia otitis.

Biometric indices and size at first sexual maturity of eight alien fish species from Bangladesh

Directory of Open Access Journals (Sweden)

Md. Yeamin Hossain

2016-09-01

Full Text Available The biometric indices and size at first sexual maturity of eight alien fish species from several water bodies in Bangladesh were studied for the first time. A total of 273 individuals of eight alien fish species (Barbonymus gonionotus, Clarias gariepinus, Ctenopharyngodon idella, Cyprinus carpio, Hypophthalmichthys molitrix, H. nobilis, Oreochromis niloticus and Pangasianodon hypophthalmus were collected using traditional fishing gears from June 2014 to May 2015. Among the four condition factors (Allometric condition factor, Fulton’s condition factor, Relative condition factor, and Relative weight studied, Fulton’s condition factor was the best for assessing the well-being of these alien species in their natural habitat, based on the relationships of condition factors with body weight and total length. The calculated form factor was 0.0270 for B. gonionotus, 0.0077 for C. gariepinus, 0.0119 for C. idella, 0.0194 for C. carpio, 0.0101 for H. molitrix, 0.0092 for H. nobilis, 0.0158 for O. niloticus and 0.0105 for P. hypophthalmus. The size at first sexual maturity was estimated in TL as 12.30 cm for B. gonionotus, 25.53 cm for C. gariepinus, 32.80 cm for C. idella, 18.22 cm for C. carpio, 23.92 cm for H. molitrix, 30.18 cm for H. nobilis, 21.78 cm for O. niloticus, and 21.32 cm for P. hypophthalmus. The present study also calculates form factor and first sexual maturity of these alien species from different water-bodies world over. The findings of this study can be very helpful for sustainable management of these alien species in Bangladesh and similar ecosystems.

Evaluation of nitrogen sources (15 N) in three wheat varieties in an andisol and in an ultisol, IX region. 1. Effect of yield, absorption and N efficiency

International Nuclear Information System (INIS)

Peyrelongue, A.; Pino, Y.; Buneder, M.

1997-01-01

Full text: During 1988/1989 the effect of nitrate and ammoniacal fertilization was studied on yield, yield components, absorption and efficiency of N according the conventional methods in three wheat varieties. The field evaluation was done in an Ultisol and Andisol of the IX Region of Chile. In both soils the statistical design was completely randomized blocks where the sources of N were the treatment: sodium nitrate, urea and ammonium nitrate. In the Andisol the wheat variety used was Laurel and in the Ultisol Dalcahue and Perquenco varieties were used. The rate of N was 160 kg N ha -1 . The application of N had a significant effect on yield in the three environments. For Dalcahue this effect was obtained with sodium nitrate and for Perquenco and Laurel there was not significant differences between nitrogen sources. The results in Ultisol show different behavior between varieties, with a better response of Perquenco according N application but a lower yield in relationship with Dalcahue. The best yield was obtained with Laurel in the Andisol, also with the higher total N absorption, AE and FUE, according with the yield obtained

Current vegetation status in the area of potential laurisilva from the Monte del Agua - Erjos watershed (Tenerife, Spain after the 2007 wildfire

Directory of Open Access Journals (Sweden)

M.E. Arozena

2013-05-01

Full Text Available The capacity of Pinus canariensis to support the effect of the fire and the fact that great part of the fires happened in Canaries have concerned to forests of this species in the last decades explain the focus put in the study about fires of the pinegrove. However, few works were done on the fire in the Canarian laurel forest. The fire of August 2007 in Tenerife affected a sector of the potential area of laurel forest, in the west of the island, and provided the opportunity of an approximation to the knowledge of the way in which the fire concerns this type of forest. The study area has a mosaic of different plant communities ecologically and floristically related to the laurel forest: a structured forest, dominated by evergreen broadleaf species, Erica arborea scrubs and old fields at different stages of recolonization after the abandonment of crops, as well as small areas with Pinus canariensis and Pinus radiata plantations. Through analysis of the floristic composition, of the vegetation physiognomy and the post-fire reproduction systems by species in forest and bush areas, a very active regeneration has been found in all situations. The importance of "self-succession" as a process of regeneration is remarkable, with a domination of the asexual reproduction in the forest areas and the combination of this one with the sexual one in those of the bushes formations. So, in the same way that happens with other types of vegetation, has been observed that the reaction of the laurel forest is very related with the features of the vegetation pre-fire, at least when the fire does not concern the soil, as in this case.

North American Lauraceae: terpenoid emissions, relative attraction and boring preferences of redbay ambrosia beetle, Xyleborus glabratus (coleoptera: curculionidae: scolytinae.

Directory of Open Access Journals (Sweden)

Paul E Kendra

Full Text Available The invasive redbay ambrosia beetle, Xyleborus glabratus, is the primary vector of Raffaelea lauricola, a symbiotic fungus and the etiologic agent of laurel wilt. This lethal disease has caused severe mortality of redbay (Persea borbonia and swampbay (P. palustris trees in the southeastern USA, threatens avocado (P. americana production in Florida, and has potential to impact additional New World species. To date, all North American hosts of X. glabratus and suscepts of laurel wilt are members of the family Lauraceae. This comparative study combined field tests and laboratory bioassays to evaluate attraction and boring preferences of female X. glabratus using freshly-cut bolts from nine species of Lauraceae: avocado (one cultivar of each botanical race, redbay, swampbay, silkbay (Persea humilis, California bay laurel (Umbellularia californica, sassafras (Sassafras albidum, northern spicebush (Lindera benzoin, camphor tree (Cinnamomum camphora, and lancewood (Nectandra coriacea. In addition, volatile collections and gas chromatography-mass spectroscopy (GC-MS were conducted to quantify terpenoid emissions from test bolts, and electroantennography (EAG was performed to measure olfactory responses of X. glabratus to terpenoids identified by GC-MS. Significant differences were observed among treatments in both field and laboratory tests. Silkbay and camphor tree attracted the highest numbers of the beetle in the field, and lancewood and spicebush the lowest, whereas boring activity was greatest on silkbay, bay laurel, swampbay, and redbay, and lowest on lancewood, spicebush, and camphor tree. The Guatemalan cultivar of avocado was more attractive than those of the other races, but boring response among the three was equivalent. The results suggest that camphor tree may contain a chemical deterrent to boring, and that different cues are associated with host location and host acceptance. Emissions of α-cubebene, α-copaene, α-humulene, and

North American Lauraceae: terpenoid emissions, relative attraction and boring preferences of redbay ambrosia beetle, Xyleborus glabratus (coleoptera: curculionidae: scolytinae).

Science.gov (United States)

Kendra, Paul E; Montgomery, Wayne S; Niogret, Jerome; Pruett, Grechen E; Mayfield, Albert E; MacKenzie, Martin; Deyrup, Mark A; Bauchan, Gary R; Ploetz, Randy C; Epsky, Nancy D

2014-01-01

The invasive redbay ambrosia beetle, Xyleborus glabratus, is the primary vector of Raffaelea lauricola, a symbiotic fungus and the etiologic agent of laurel wilt. This lethal disease has caused severe mortality of redbay (Persea borbonia) and swampbay (P. palustris) trees in the southeastern USA, threatens avocado (P. americana) production in Florida, and has potential to impact additional New World species. To date, all North American hosts of X. glabratus and suscepts of laurel wilt are members of the family Lauraceae. This comparative study combined field tests and laboratory bioassays to evaluate attraction and boring preferences of female X. glabratus using freshly-cut bolts from nine species of Lauraceae: avocado (one cultivar of each botanical race), redbay, swampbay, silkbay (Persea humilis), California bay laurel (Umbellularia californica), sassafras (Sassafras albidum), northern spicebush (Lindera benzoin), camphor tree (Cinnamomum camphora), and lancewood (Nectandra coriacea). In addition, volatile collections and gas chromatography-mass spectroscopy (GC-MS) were conducted to quantify terpenoid emissions from test bolts, and electroantennography (EAG) was performed to measure olfactory responses of X. glabratus to terpenoids identified by GC-MS. Significant differences were observed among treatments in both field and laboratory tests. Silkbay and camphor tree attracted the highest numbers of the beetle in the field, and lancewood and spicebush the lowest, whereas boring activity was greatest on silkbay, bay laurel, swampbay, and redbay, and lowest on lancewood, spicebush, and camphor tree. The Guatemalan cultivar of avocado was more attractive than those of the other races, but boring response among the three was equivalent. The results suggest that camphor tree may contain a chemical deterrent to boring, and that different cues are associated with host location and host acceptance. Emissions of α-cubebene, α-copaene, α-humulene, and calamenene were

78 FR 66422 - Appointment of Members of the Legal Division to the Performance Review Board, Internal Revenue...

Science.gov (United States)

2013-11-05

... Counsel (Operations) 2. Laurel Robinson, Deputy Division Counsel (Large Business & International) 3. Debra... Counsel (Passthroughs and Special Industries) 5. Andrew Keyso, Associate Chief Counsel (Income Tax...

HYDROLOGY, LAUREL COUNTY, KENTUCKY USA

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — Hydrology data include spatial datasets and data tables necessary for documenting the hydrologic procedures for estimating flood discharges for a flood insurance...

Loss of Functional Photosystem II Reaction Centres in Zooxanthellae of Corals Exposed to Bleaching Conditions: Using Fluorescence Rise Kinetics.

Science.gov (United States)

Hill, R; Larkum, A W D; Frankart, C; Kühl, M; Ralph, P J

2004-01-01

Mass coral bleaching is linked to elevated sea surface temperatures, 1-2 degrees C above average, during periods of intense light. These conditions induce the expulsion of zooxanthellae from the coral host in response to photosynthetic damage in the algal symbionts. The mechanism that triggers this release has not been clearly established and to further our knowledge of this process, fluorescence rise kinetics have been studied for the first time. Corals that were exposed to elevated temperature (33 degrees C) and light (280 mumol photons m(-2) s(-1)), showed distinct changes in the fast polyphasic induction of chlorophyll-a fluorescence, indicating biophysical changes in the photochemical processes. The fluorescence rise over the first 2000ms was monitored in three species of corals for up to 8 h, with a PEA fluorometer and an imaging-PAM. Pocillopora damicornis showed the least impact on photosynthetic apparatus, while Acropora nobilis was the most sensitive, with Cyphastrea serailia intermediate between the other two species. A. nobilis showed a remarkable capacity for recovery from bleaching conditions. For all three species, a steady decline in the slope of the initial rise and the height of the J-transient was observed, indicating the loss of functional Photosystem II (PS II) centres under elevated-temperature conditions. A significant loss of PS II centres was confirmed by a decline in photochemical quenching when exposed to bleaching stress. Non-photochemical quenching was identified as a significant mechanism for dissipating excess energy as heat under the bleaching conditions. Photophosphorylation could explain this decline in PS II activity. State transitions, a component of non-photochemical quenching, was a probable cause of the high non-photochemical quenching during bleaching and this mechanism is associated with the phosphorylation-induced dissociation of the light harvesting complexes from the PS II reaction centres. This reversible process may

Fulltext PDF

Indian Academy of Sciences (India)

mounted on a slender intensely red stalk and arranged into long , drooping , candelabra-like sprays. Stamens are ten with long filaments. The young pods are brilliant crimson. The plant's name was given in honour of Sarah, Countess of Amherst, wife of the governor of Myanmar; nobilis, on account of the exquisite beauty of ...

75 FR 28235 - Export Trade Certificate of Review

Science.gov (United States)

2010-05-20

..., Dallas, TX (controlling entity: Sekisui America Corporation, Mt. Laurel, NJ); Thomas M. Johnson, Park... Members from USSA's Certificate: Atotech USA, Inc., Rockhill, SC; Bostik, Inc., Wauwatosa, WI; Cook...

Drug: D06712 [KEGG MEDICUS

Lifescience Database Archive (English)

Full Text Available [TAX:119260] ... Same as: E00084 E00567 Therapeutic category: 5100 ... Lauraceae (laurel family) Cinnamon bark (dried) Major component: Cinnamaldehyde [CPD:C00903] ... PubChem: 47208363 ...

75 FR 50747 - Export Trade Certificate of Review

Science.gov (United States)

2010-08-17

..., Dallas, TX (controlling entity: Sekisui America Corporation, Mt. Laurel, NJ); and Thomas M. Johnson, Park... Members from USSA's Certificate: Atotech USA, Inc., Rockhill, SC; Bostik, Inc., Wauwatosa, WI; Cook...

Chemical composition and fumigant toxicity of the essential oils from 16 species of Eucalyptus against Haematobia irritans (Diptera: Muscidae) adults

OpenAIRE

Juan, Laura W.; Lucía Mulas, Alejandro; Zerba, Eduardo N.; Harrand, Leonel; Marco, Martín; Masuh, Hector M.

2011-01-01

Oils extracted from various species of Eucalyptus (Eucalyptus badjensis Beuzev & Welch, Eucalyptus badjensis x Eucalyptus nitens, Eucalyptus benthamii variety dorrigoensis Maiden & Cambage, Eucalyptus botryoides Smith, Eucalyptus dalrympleana Maiden, Eucalyptus fastigata Deane & Maiden, Eucalyptus nobilis L.A.S. Johnson & K. D. Hill, Eucalyptus polybractea R. Baker, Eucalyptus radiata ssp. radiata Sieber ex Spreng, Eucalyptus resinifera Smith, Eucalyptus robertsonii Blakely, Eucalyptus rubida...

Environ: E00650 [KEGG MEDICUS

Lifescience Database Archive (English)

Full Text Available ame as: D06713 Lauraceae (laurel family) Cinnamomum cassia the dried leaves, twigs, and bark Obtained by steam distillation Major component: Cinnamaldehyde [CPD:C00903] CAS: 8007-80-5

Resilience Assessment of Lowland Plantations Using an Ecosystem Modeling Approach

Directory of Open Access Journals (Sweden)

Chia-Hsin Wu

2015-03-01

Full Text Available As afforestation programs of former farmlands take hold in Taiwan to achieve a variety of ecological and socio-economic values, it is becoming necessary to define best forest management. Hence, we simulated mixed stands of Cinnamomum camphora and Fraxinus griffithii planted through a gradient of soil fertility and varying camphor/ash density ratios, but maintaining a fixed total stand density of 1500 trees ha−1. Total stand productivity was slightly lower in mixed stands than the combination of both monocultures in rich and poor sites. Maximum negative yield surpluses for 50-year old stands were 7 Mg ha−1 and 6 Mg ha−1 for rich and poor sites with a 1:1 camphor laurel/ash ratios. Maximum stand woody biomass in rich sites was reached in camphor laurel monocultures (120 Mg ha−1 and in poor sites for Himalayan ash monocultures (58 Mg ha−1. However, for medium-quality sites, a small yield surplus (11 Mg ha−1 was estimated coinciding with a maximum stand woody biomass of 95 Mg ha−1 for a 1:1 camphor laurel/ash density ratio. From an ecological resilience point of view, rotation length was more important than stand composition. Long rotations (100 years could improve soil conditions in poor sites. In rich sites, short rotations (50 years should be avoided to reduce risks or fertility loss.

Phenolic composition of selected herbal infusions and their anti-inflammatory effect on a colonic model in vitro in HT-29 cells

Directory of Open Access Journals (Sweden)

Elda Herrera-Carrera

2015-12-01

Full Text Available Some herbal infusions used in folk medicine in Mexico to treat gastrointestinal disorders were evaluated. Antioxidant activity and phenolic compounds were analyzed on the lyophilized aqueous crude extracts (LACE of arnica (Aster gymnocephalus, chamomile (Chamaemelum nobile, cumin (Cominum cyminum, desert resurrection plant (DRP (Selaginella lepidophylla, laurel (Listea glaucescens, marjoram (Origanum majorana, mint (Mentha spicata, salvilla (Buddleia scordioides and yerbaniz (Tagetes lucida. Total phenolic content ranged from 8.0 to 70.7 μg GAE/mg for DRP and laurel respectively. Major phenolic compounds were identified by gas chromatography–mass spectrometry and high-performance liquid chromatography. The IC50 determined by the degradation of the deoxy-d-ribose ranged from 2,452.53 to 5,097.11 μg/mL. The cytoprotective effect of the LACE alone and on indomethacin-induced oxidative stress in HT-29 cells was tested. The tetrazolium dye MTT assay was performed in concentrations of 0.125–10 mg/mL allowing choosing the lowest concentration for this experimentation. Inflammation markers were measured by Western blotting. None of the extracts inhibited COX-1 by themselves; however, it was observed that extracts have a modulation effect over COX-2, TNFα, NFκB, and IL-8. By the decrease in the expression of pro-inflammatory cytokines, it follows that salvilla, chamomile, and laurel show promising anti-inflammatory effects.

Comparing Avocado, Swamp Bay, and Camphortree as Hosts of Raffaelea lauricola Using a Green Fluorescent Protein (GFP)-Labeled Strain of the Pathogen.

Science.gov (United States)

Campbell, A S; Ploetz, R C; Rollins, J A

2017-01-01

Raffaelea lauricola, a fungal symbiont of the ambrosia beetle Xyleborus glabratus, causes laurel wilt in members of the Lauraceae plant family. North American species in the family, such as avocado (Persea americana) and swamp bay (P. palustris), are particularly susceptible to laurel wilt, whereas the Asian camphortree (Cinnamomum camphora) is relatively tolerant. To determine whether susceptibility is related to pathogen colonization, a green fluorescent protein-labeled strain of R. lauricola was generated and used to inoculate avocado, swamp bay, and camphortree. Trees were harvested 3, 10, and 30 days after inoculation (DAI), and disease severity was rated on a 1-to-10 scale. By 30 DAI, avocado and swamp bay developed significantly more severe disease than camphortree (mean severities of 6.8 and 5.5 versus 1.6, P < 0.003). The extent of xylem colonization was recorded as the percentage of lumena that were colonized by the pathogen. More xylem was colonized in avocado than camphortree (0.9% versus 0.1%, P < 0.03) but colonization in swamp bay (0.4%) did not differ significantly from either host. Although there were significant correlations between xylem colonization and laurel wilt severity in avocado (r = 0.74), swamp bay (r = 0.82), and camphortree (r = 0.87), even severely affected trees of all species were scarcely colonized by the pathogen.

Sensory and chemical stability in coated peanuts with the addition of essential oils and synthetic antioxidants

Directory of Open Access Journals (Sweden)

Olmedo, R. H.

2012-03-01

Full Text Available The objective of this work was to evaluate the antioxidant effect of essential oils on the oxidative stability of coated peanuts. Untreated coated peanuts (CP and treated coated peanuts with the addition of rosemary (CP-R, oregano (CP-O and laurel (CP-L essential oils and BHT (CPBHT were prepared. Peroxide values (PV and p-anisidine values (AV and the intensity ratings of sensory attributes by descriptive analysis were measured during 112 days of storage at room temperature (23°C. CP-BHT exhibited the lowest PV and AV increase. CP-R, CP-O and CP-L showed lower rates of increase in PV and AV than CP. The oxidized and cardboard flavor intensity ratings increased much more in CP during storage than the other studied products. CPBHT also showed the lowest increase in the intensity ratings of these sensory attributes. Three essential oils, namely, laurel, oregano and rosemary showed antioxidant activity and increased the shelf life of coated peanuts.

El objetivo de este trabajo fue evaluar el efecto antioxidante de aceites esenciales sobre la estabilidad oxidativa en maní recubiertos. Se prepararon maníes recubiertos sin agregados (CP, y con el agregado de aceites esenciales de romero (CP-R, orégano (CP-O y laurel (CP-L y BHT (CPBHT. Se midieron, durante 112 días de almacenamiento, los valores de peróxidos (PV y p-anisidina (AV, y las intensidades de atributos sensoriales mediante análisis descriptivo. CP-BHT presentó el menor valor de PV y AV. CP-R, CP-O y CP-L tuvieron mayor PV y AV respecto a CP. Los valores de intensidad del sabor oxidado y cartón tuvieron un mayor incremento en CP durante el almacenamiento con respecto a los otros productos estudiados. La muestra CP-BHT también mostró los menores valores de intensidad de estos atributos sensoriales. Los aceites esenciales de laurel, orégano y romero presentaron actividad antioxidante e incrementaron la vida útil del maní recubierto.

Determination of volatiles produced during radiation processing in food and medicinal herbs

International Nuclear Information System (INIS)

Salum, Debora Christina

2008-01-01

In order to protect food from pathogenic microorganisms as well as to increase its shelf life while keeping sensorial properties (e.g. odor and taste), once the latter are one of the main properties required by spice buyers, it is necessary to analyze volatile formation from irradiation of medicinal and food herbs. The aim of the present study was to analyze volatile formation from Co irradiation of Laurus Cinnamomum, Piper Nigrum, Origanum Vulgare and Myristica Fragans. Possible changes on the odor of these herbs are evaluated by characterizing different radiation doses and effects on sensorial properties in order to allow better application of irradiation technology. l he samples have been irradiated in plastic packages by making use of a 60 Co Gamma irradiator. Irradiation doses of 0, 5, 10, 15, 20 and 25kGy have been tested. For the analysis of the samples, SPME has been applied, while for the analysis of volatile compounds, CG/MS. Spice irradiation has promoted mostly decrease in volatile compounds when doses of 5, 10, 15, 20 and 25kGy were used. For Laurus cinnamomum, the irradiation decreased volatile by nearly 56% and 89.5% respectively, comparing to volatile from a sample which has not been previously irradiated. Differently from other spices analyzed, irradiation on Myristica Fragans has increased volatile compounds except for 4-terpineol. The miristicine (toxic substance when in large quantities, commonly mentioned as narcotic) has increased by nearly 80%. For Origanum Vulgare and Piper Nigrum, significant decrease in volatile compounds have been found, mainly when it comes to 25 kGy irradiation. In general, results indicate loss of sensorial quality of spices. (author)

Determination of volatiles produced during radiation processing in food and medicinal herbs; Determinacao de volateis produzidos durante o processamento por radiacao em ervas alimenticias e medicinais

Energy Technology Data Exchange (ETDEWEB)

Salum, Debora Christina

2008-07-01

In order to protect food from pathogenic microorganisms as well as to increase its shelf life while keeping sensorial properties (e.g. odor and taste), once the latter are one of the main properties required by spice buyers, it is necessary to analyze volatile formation from irradiation of medicinal and food herbs. The aim of the present study was to analyze volatile formation from Co irradiation of Laurus Cinnamomum, Piper Nigrum, Origanum Vulgare and Myristica Fragans. Possible changes on the odor of these herbs are evaluated by characterizing different radiation doses and effects on sensorial properties in order to allow better application of irradiation technology. l he samples have been irradiated in plastic packages by making use of a {sup 60}Co Gamma irradiator. Irradiation doses of 0, 5, 10, 15, 20 and 25kGy have been tested. For the analysis of the samples, SPME has been applied, while for the analysis of volatile compounds, CG/MS. Spice irradiation has promoted mostly decrease in volatile compounds when doses of 5, 10, 15, 20 and 25kGy were used. For Laurus cinnamomum, the irradiation decreased volatile by nearly 56% and 89.5% respectively, comparing to volatile from a sample which has not been previously irradiated. Differently from other spices analyzed, irradiation on Myristica Fragans has increased volatile compounds except for 4-terpineol. The miristicine (toxic substance when in large quantities, commonly mentioned as narcotic) has increased by nearly 80%. For Origanum Vulgare and Piper Nigrum, significant decrease in volatile compounds have been found, mainly when it comes to 25 kGy irradiation. In general, results indicate loss of sensorial quality of spices. (author)

Table 2

African Journals Online (AJOL)

mcabuk

Effect of a dietary essential oil mixture on performance of laying hens ... Keywords: Essential oils, mannan oligosaccharide, antibiotic, egg production, laying hen, .... essential oils derived from selected herbs: Oregano oil (Origanum sp.), laurel ...

77 FR 59081 - Technical Amendments Due to Change of Agency Name

Science.gov (United States)

2012-09-26

... day such act is transmitted by the Chairman to the Speaker of the House of Representatives and the... right superimposed upon the United States Capitol dome and two laurel branches both in gold which appear...

Annual North Dakota Elevator Marketing Report, 2008-09

Science.gov (United States)

2009-12-01

The Annual North Dakota Elevator Marketing Report for 2008-09 was prepared by Kimberly Vachal and Laurel Benson, : Upper Great Plains Transportation Institute. The authors gratefully acknowledge the assistance of the North Dakota : Grain Dealers Asso...

Annual North Dakota Elevator Marketing Report, 2009-10

Science.gov (United States)

2010-11-01

The Annual North Dakota Elevator Marketing Report for 2009-10 was prepared by Kimberly Vachal and Laurel Benson, : Upper Great Plains Transportation Institute. The authors gratefully acknowledge the assistance of the North Dakota : Wheat Commission a...

Annual North Dakota Elevator Marketing Report, 2007-08

Science.gov (United States)

2008-12-01

The Annual North Dakota Elevator Marketing Report for 2007-08 was prepared by Kimberly Vachal and Laurel Benson, : Upper Great Plains Transportation Institute. The authors gratefully acknowledge the assistance of the North Dakota : Grain Dealers Asso...

Final Remedial Investigation Report Area of Contamination (AOC) 57. Volume I. Text Sections 1 Through 10, Figures and Tables

Science.gov (United States)

2000-06-01

choke cherry ( Prunus virginiana ), maleberry (Lyonia ligustrina), and paper birch (Betula papyrifera). Herbaceous species observed in this habitat...portion of this habitat include sheep laurel, witch hazel (Hamamelis virginiana ), nannyberry, choke cherry, clubmoss (Lycopodium carolinianum), and ferns

Annual North Dakota Elevator Marketing Report, 2010-11

Science.gov (United States)

2011-12-01

The Annual North Dakota Elevator Marketing Report for 2010-11 was prepared by Kimberly Vachal and Laurel Benson, Upper Great Plains Transportation Institute. The authors gratefully acknowledge the assistance of the North Dakota Wheat Commission and t...

Is stump sprout treatment necessary to effectively control Phytophthora ramorum in California's wildlands?

Science.gov (United States)

Yana Valachovic; Richard Cobb; David Rizzo; Brendan Twieg; Chris Lee; Radoslaw Glebocki

2013-01-01

In California, wildland hosts that support sporulation of Phytophthora ramorum, such as California bay laurel (Umbellularia californica (Hook. & Arn.) Nutt.) and tanoak (Notholithocarpus densiflorus (Hook. & Arn.) Manos, Cannon & S.H. Oh), also develop prolific basal sprouts following...

South African Medical Journal - Vol 104, No 10 (2014)

African Journals Online (AJOL)

Izindaba: Ebola: SA has no outbreak 'laurels' to rest on · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. C Bateman, 653-655. http://dx.doi.org/10.7196/SAMJ.8894 ...

African Journal of Biotechnology - Vol 10, No 72 (2011)

African Journals Online (AJOL)

Phenolic composition and antioxidant capacity of Cherry laurel (Laurocerasus officinalis Roem.) sampled from Trabzon region, Turkey · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. FY Karahalil, H Şahin, 16293-16299 ...

Evaluation of lure combinations containing essential oils and volatile spiroketals for detection of host-seeking Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae)

Science.gov (United States)

The invasive redbay ambrosia beetle, Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae: Scolytinae) vectors the fungal pathogen (Raffaelea lauricola) that causes laurel wilt, a disease responsible for widespread mortality of trees in the Lauraceae in the southeastern U.S. Early detection of in...

Factors influencing Phytophthora ramorum infectivity on Umbellularia californica and testing of a defoliation-based control method

Science.gov (United States)

Christine Windsor Colijn; Michael Cohen; Steve Johnston; Whalen Dillon; Nathan Rank

2013-01-01

The primary foliar host for Phytophthora ramorum is California bay laurel, Umbellularia californica (Hook. & Arn.) Nutt., a main reservoir for the pathogen in California woodlands. We investigated environmental and pathogen-mediated influences on incidence and severity of P. ramorum infection of

Morpho-anatomical features of underground systems in six Asteraceae species from the Brazilian Cerrado.

Science.gov (United States)

Appezzato-da-Glória, Beatriz; Cury, Graziela

2011-09-01

In the Brazilian Cerrado (neotropical savanna), the development of bud-bearing underground systems as adaptive structures to fire and dry periods can comprise an important source of buds for this ecosystem, as already demonstrated in the Brazilian Campos grasslands and North American prairies. Asteraceae species from both woody and herbaceous strata have subterranean organs that accumulate carbohydrates, reinforcing the adaptive strategy of these plants to different environmental conditions. This study aims to analyse the morpho-anatomy of underground systems of six species of Asteraceae (Mikania cordifolia L.f. Willd., Mikania sessilifolia DC, Trixis nobilis (Vell.) Katinas, Pterocaulon alopecuroides (Lam.) DC., Vernonia elegans Gardner and Vernonia megapotamica Spreng.), to describe these structures and to verify the occurrence and origin of shoot buds, and to analyse the presence of reserve substances. Individuals sampled in Cerrado areas in São Paulo State showed thick underground bud-bearing organs, with adventitious or lateral roots and presence of fructans. Xylopodium was found in all studied species, except for Trixis nobilis, which had stem tuber. The presence of fructans as reserve, and the capacity of structures in the formation of buds indicate the potential of herbaceous species of Asteraceae in forming a viable bud bank for vegetation regeneration in the Brazilian Cerrado.

2014 USGS/NRCS Lidar: Central MS

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — TASK NAME: USGS-NRCS Laurel MS 0.7m NPS LIDAR Lidar Data Acquisition and Processing Production Task USGS Contract No. G10PC00057 Task Order No. G13PD01086 Woolpert...

Highly productive continuous miners

Energy Technology Data Exchange (ETDEWEB)

Sanda, A.P.

1993-12-01

Profiles operations at 4 Appalachian underground coal mines. Mines covered are: Laurel Coal Corp., Danville, W. Va; Sunrise Mining Inc., Jolo W.Va.; Unicorn Mining, Inc., London, Ky; and Reedy Coal Co., Deane, Ky. Describes mining methods and equipment. 2 tabs., 8 photos.

75 FR 67388 - Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval

Science.gov (United States)

2010-11-02

... toll-free number). Persons with hearing or speech impairments may access that number through TTY by... Office Termination Mortgagee name address jurisdictions effective date Homeownership centers Freedom.... Freedom Mortgage Corp......... 907 Pleasant Valley Des Moines....... 10/4/10 Denver. Ave. Mount Laurel, NJ...

Use of different spices as potential natural antioxidant additives on cooked beans (Phaseolus vulgaris). Increase of DPPH radical scavenging activity and total phenolic content.

Science.gov (United States)

Pereira, Marina Pelincer; Tavano, Olga Luisa

2014-12-01

Herbs and spices, excellent sources of phenolic compounds, can be considered potential antioxidant additives. The use of spices must strike a balance between their potential antioxidant capabilities during preparation and the flavor acceptance, in order to avoid rejection of the food. The aimed of this study is to evaluate the influence of different spices and their concentrations on cooked common beans, focusing its potential as antioxidant additives. Onion, parsley, spring onion, laurel and coriander increased the antioxidant activity of preparation when used at 7.96 g of onion, 1.06 g parsley, 3.43 g spring onion, 0.25 g laurel (dry leaves), and 0.43 g coriander/100 g of cooked beans. Besides, these spices concentrations enhance total phenolics and alter the mixture protein digestibility minimally. For garlic samples it was not possible to establish a concentration that increases the antioxidant activity of cooked beans.

Kvantitativní analýza léčiv v biologických materiálech separačními metodami

OpenAIRE

Maláková, Jana

2012-01-01

Charles University in Prague, Faculty of Pharmacy in Hradci Králové Department of Pharmaceutical Chemistry and Drug Control Candidate: PharmDr. Jana Maláková Supervisor: Doc. PharmDr. Milan Nobilis, PhD., Associate Professor of Bioorganic Chemistry. Title of Doctoral Thesis: Quantitative measurement of drugs in biological samples using chromatography methods. The thesis deals with the application of instrumental analytical methods to the quantitative measurement of drugs and their metabolites...

Plant succession and community restoration following felling and burning in the Southern Appalachian Mountains

Science.gov (United States)

Barton D. Clinton; James M. Vose

2000-01-01

Recent declines in the yellow pine component of pine-hardwood stands in the Southern Appalachian Mountains has prompted managers to increase the use of fire as a silvicultural tool. The fell and burn treatment is designed to remove competing vegetation (hardwoods and mountain laurel...

Contact allergy to spices

NARCIS (Netherlands)

W. Van den Akker Th. (W.); I.D. Roesyanto-Mahadi (I.); A.W. van Toorenenbergen (Albert); Th. van Joost (Theo)

1990-01-01

textabstractA group of 103 patients suspected of contact allergy was tested with the European standard series, wood tars and spices; paprika, cinnamon, laurel, celery seed, nutmeg, curry, black pepper, cloves, while pepper, coriander, cacao and garlic. 32 patients (Group I) were selected on the

75 FR 37288 - Kiwifruit Grown in California; Order Amending Marketing Order No. 920

Science.gov (United States)

2010-06-29

... administration of the order. The amendments will redefine the grower districts into which the production area is... improve the operation and administration of the California kiwifruit marketing order program. Proposed..., 2010. FOR FURTHER INFORMATION CONTACT: Laurel May or Kathleen M. Finn, Marketing Order Administration...

Chemical ecology and serendipity: Developing attractants for Florida ambrosia beetle pests

Science.gov (United States)

Two exotic ambrosia beetles have become established in southern Florida: Xyleborus glabratus, the redbay ambrosia beetle (RAB), and Euwallacea fornicatus, the tea shot hole borer (TSHB). Both pests vector pathogenic fungal symbionts; the former for laurel wilt and the latter for Fusarium dieback d...

Screening of bacteria for antagonistic activity against phytopathogens of avocados

Science.gov (United States)

Bacteria and fungi were isolated from the bark of the avocado tree (Persea americana) located in southern Florida. The bacterial strains were subsequently assayed for antagonism activity against Raffaelea lauricola, the causal agent of laurel wilt in avocados. The screen identified no isolates that ...

Chemical ecology and lure development for redbay ambrosia beetle

Science.gov (United States)

The exotic redbay ambrosia beetle, Xyleborus glabratus Eichhoff, has become a serious invasive pest in the U.S., currently established in nine southeastern states. Female beetles are the primary vectors of a pathogenic fungus (Raffaelea lauricola) that causes laurel wilt. This lethal vascular dise...

Suppression of Phytophthora ramorum infestations through silvicultural treatment in California's north coast

Science.gov (United States)

Yana Valachovic; Chris Lee; Brendan Twieg; David Rizzo; Richard Cobb; Radoslaw Glebocki

2013-01-01

In 2006, three forested sites infested with Phytophthora ramorum in Humboldt County, California were subjected to different combinations of treatments designed to reduce inoculum and control spread. One treatment, consisting of removal of all California bay laurel (Umbellularia californica (Hook. & Arn.) Nutt.) and tanoak...

Morpho-anatomical features of underground systems in six Asteraceae species from the Brazilian Cerrado

Directory of Open Access Journals (Sweden)

Beatriz Appezzato-da-Glória

2011-09-01

Full Text Available In the Brazilian Cerrado (neotropical savanna, the development of bud-bearing underground systems as adaptive structures to fire and dry periods can comprise an important source of buds for this ecosystem, as already demonstrated in the Brazilian Campos grasslands and North American prairies. Asteraceae species from both woody and herbaceous strata have subterranean organs that accumulate carbohydrates, reinforcing the adaptive strategy of these plants to different environmental conditions. This study aims to analyse the morpho-anatomy of underground systems of six species of Asteraceae (Mikania cordifolia L.f. Willd., Mikania sessilifolia DC, Trixis nobilis (Vell. Katinas, Pterocaulon alopecuroides (Lam. DC., Vernonia elegans Gardner and Vernonia megapotamica Spreng., to describe these structures and to verify the occurrence and origin of shoot buds, and to analyse the presence of reserve substances. Individuals sampled in Cerrado areas in São Paulo State showed thick underground bud-bearing organs, with adventitious or lateral roots and presence of fructans. Xylopodium was found in all studied species, except for Trixis nobilis, which had stem tuber. The presence of fructans as reserve, and the capacity of structures in the formation of buds indicate the potential of herbaceous species of Asteraceae in forming a viable bud bank for vegetation regeneration in the Brazilian Cerrado.No Cerrado brasileiro (savana neotropical, o desenvolvimento de sistemas subterrâneos que produzem gemas, como estruturas adaptativas contra o fogo e períodos de seca, pode compreender um importante suprimento de gemas para esse ecossistema, como já demonstrado nos campos brasileiros e nas pradarias norte-americanas. Espécies de Asteraceae tanto do estrato lenhoso, quanto do herbáceo têm órgãos que acumulam carboidratos, reforçando a estratégia adaptativa dessas plantas a diferentes condições ambientais. Este estudo tem o objetivo de analisar a morfo

75 FR 33736 - Tart Cherries Grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah, Washington...

Science.gov (United States)

2010-06-15

...., Stop 0237, Washington, DC 20250-0237; telephone: (202) 720-2491, Fax: (202) 720-8938, or E-mail: Laurel... DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 930 [Doc. No. AO-370-A8; AMS..., Oregon, Utah, Washington, and Wisconsin; Withdrawal of Proposed Rule AGENCY: Agricultural Marketing...

The Strict Liability Principle and the Human Rights of the Athlete in Doping Cases

NARCIS (Netherlands)

J.W. Soek

2006-01-01

textabstractAthletes who achieve extraordinary feats on the pitch stir up the imagination and enjoy a unique position within society. However, laurels received one day, may be just as quickly snatched back the next if it becomes known that the athlete achieved his or her exceptional performance

Response of Planted Eastern White Pine (Pinus strobus L.) to Mechanical Release, Competition, and Drought in the Southern Appalachians

Science.gov (United States)

Barton D. Clinton; Katherine J. Elliott; Wayne T. Swank

1997-01-01

Conversion of low-quality, natural mixed pine hardwood ecosystems, containing a mountain laurel (Kalmia latifolia L.) dominated understory, to more productive eastern white pine (Pinus strobus L.)/mixed-hardwood systems is a common prescription on relatively xeric southern Appalachian forest sites. We examined the effects of...

Anatomia del nervo faciale

OpenAIRE

Barbut , J.; Tankere , F.; Bernat , I.

2017-01-01

International audience; Il nervo faciale è al centro della pratica quotidiana in oto-rino-laringoiatria. La sua singolare fisiologia e la sua patologia fanno di questo paio di nervi cranici un soggetto appassionante in cui alcuni si sono specializzati. La precisa conoscenza della sua anatomia, il cui percorso è tortuoso e presenta molte relazioni con altri elementi nobili, è un prerequisito indispensabile per il suo approccio, sia in chirurgia cervicale che in quella otologica che in quella n...

Phase Change Energy Storage Material Suitable for Solar Heating System

Science.gov (United States)

Li, Xiaohui; Li, Haihua; Zhang, Lihui; Liu, Zhenfa

2018-01-01

Differential scanning calorimetry (DSC) was used to investigate the thermal properties of palmitic acid, myristic acid, laurel acid and the binary composite of palmitic/laurel acid and palmitic/myristic acid. The results showed that the phase transition temperatures of the three monomers were between 46.9-65.9°C, and the latent heats were above 190 J/g, which could be used as solar energy storage material. When the mass ratio of Palmitic acid and myristic was 1:1, the eutectic mixture could be formed. The latent heat of the eutectic mixture was 186.6 J/g, the melting temperature and the solidification temperature was 50.6°C and 43.8°C respectively. The latent heat of phase change and the melting temperature had not obvious variations after 400 thermal cycles, which proved that the binary composite had good thermal stability and was suitable for solar floor radiant heating system.

Browse Title Index

African Journals Online (AJOL)

Items 8051 - 8100 of 11090 ... Vol 10, No 72 (2011), Phenolic composition and antioxidant capacity of Cherry laurel (Laurocerasus officinalis Roem.) sampled from Trabzon region, Turkey, Abstract PDF. FY Karahalil, H Şahin. Vol 10, No 62 (2011), Phenolic compounds and antioxidant activities in some fruits and vegetables ...

Laurel Lodge Nursing Home, Templemichael Glebe, Longford.

LENUS (Irish Health Repository)

Mitchell, Kevin

2011-11-11

Abstract The number of individual cases of psychiatric disorders that can be ascribed to identified, rare, single mutations is increasing with great rapidity. Such mutations can be recapitulated in mice to generate animal models with direct etiological validity. Defining the underlying pathogenic mechanisms will require an experimental and theoretical framework to make the links from mutation to altered behavior in an animal or psychopathology in a human. Here, we discuss key elements of such a framework, including cell type-based phenotyping, developmental trajectories, linking circuit properties at micro and macro scales and definition of neurobiological phenotypes that are directly translatable to humans.

Laurel Lodge Nursing Home, Templemichael Glebe, Longford.

LENUS (Irish Health Repository)

Ivers, Mary E.

2009-01-01

Cancer survivor numbers in Ireland are increasing due to the success of modern treatments. Although most survivors have a good quality of life not all survivors return to \\'normal\\' after treatment. The HSE funded CANSURVIVOR research project has found that many survivors have difficulties and need help to recover and adjust after cancer treatment. Over a number of exploratory studies using interviews, focus groups and a survey of 262 breast, prostate, colorectal and lung cancer survivors, the researchers found that over 25% of survivors experienced significant difficulties with physical, emotional and social functioning, including symptoms such as insomnia and fatigue, while 33% experienced high levels of anxiety. Of particular concern were the findings that over 50% of survivors were overweight, 35% had reduced their physical activity levels and 13% continued to smoke after cancer, putting them at risk for further health problems. This evidence led to the development of an 8-week multi-disciplinary pilot rehabilitation programme. Significant quality of life improvements were achieved with increases in strength and fitness as well as a reduction in anxiety levels and dietary improvements. The researchers highlight the need for a structured, co-ordinated survivorship service, education of health professionals about survivorship and the provision of high quality information to survivors. This research was led by the School of Psychology at UCD in collaboration with the Physiotherapy and Nutrition departments of St. Vincent\\'s hospital.

American Women: Early Pursuit for Olympic Laurels.

Science.gov (United States)

Welch, Paula

Women were not considered in preliminary discussions concerning the events, designation of participants, competitive attire, and problems relating to amateurism in the first Olympic games. Golf was the first sport in which women participated in the Olympics, and the first woman to achieve Olympic recognition for the United States did so by winning…

Laurel Lodge Nursing Home, Templemichael Glebe, Longford.

LENUS (Irish Health Repository)

Hedden, Lindsay

2012-01-01

Among women with surgically removed, high-risk HER-2\\/neu-positive breast cancer, trastuzumab has demonstrated significant improvements in disease-free and overall survival. The objective of this study is to evaluate the cost-effectiveness of the currently recommended 12-month adjuvant protocol of trastuzumab using a Markov modeling approach and real-world cost data.

Laurels for Swiss in-service inspection team

International Nuclear Information System (INIS)

Anon.

1984-01-01

Following what is believed to be the first successful application of P-scan ultrasonic testing for the in-service inspection of austenitic welds in the boiling water reactor at Wuergassen (Federal Republic of Germany) last year, Sulzer participated in a 'round robin' test organized by the Electric Power Research Institute in the USA form May to July 1983. (Auth.)

PRELIMINARY, LAUREL COUNTY AND INCORPORATED AREAS, KENTUCKY

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — The Digital Flood Insurance Rate Map (DFIRM) Database depicts flood risk information and supporting data used to develop the risk data. The primary risk...

EVALUACIÓN DE REQUERIMIENTOS NUTRICIONALES EN VIVERO DE ESPECIES TROPICALES EMPLEADAS EN SILVICULTURA URBANA

Directory of Open Access Journals (Sweden)

David Andrés Herrera Ramírez

Full Text Available La fertilización es determinante del crecimiento y desarrollo de árboles urbanos. Por ello, estudiamos, mediante la técnica del elemento faltante, los requerimientos nutricionales de cinco especies tropicales usadas en silvicultura urbana (Tabebuia chrysantha, Margaritaria nobilis, Hamelia patens, Apeiba aspera, Cupania americana. Se encontró algunas especies sensibles a la deficiencia de nitrógeno y otras a la deficiencia de azufre. El grupo de T. chrysanta, M. nobilis y H. patens mostró alta sensibilidad a la deficiencia de nitrógeno, mientras que C. americana y A. aspera presentaron sensibilidad a la deficiencia de azufre, representada en menor crecimiento promedio para todas las variables evaluadas: altura, diámetro en el cuello de la raíz, materia seca radical y materia seca aérea. Las representaciones HJ-Biplot, utilizadas para caracterizar la respuesta por especie a cada tratamiento en todas las unidades experimentales, evidenciaron los bajos rendimientos en crecimiento para las deficiencias en nitrógeno y azufre. El desbalance de bases calcio/magnesio y magnesio/potasio afectó el crecimiento en altura de todas las especies, evidenciado en los altos crecimientos obtenidos para los tratamientos deficientes en calcio y magnesio. Debido a esto, las fórmulas nutricionales utilizadas en vivero deben reajustarse a las exigencias individuales de las especies, para producir mejores árboles para la ciudad.

Assessment of heavy metals pollution in the gulf of Gabes (Tunisia using four mollusk species

Directory of Open Access Journals (Sweden)

L. RABAOUI

2013-08-01

Full Text Available Since the establishment of the ‘Tunisian Chemical Group’ in Gabes city, Tunisia, no serious investigations were carried out about the heavy metal pollution in Gabes gulf.  In the present study, the contents of four heavy metals were assessed in four mollusk species (two gastropods, Gibbula ardens and Patella caerulea, and two bivalves, Pinctada radiata and Pinna nobilis, collected from twelve coastal stations. The results obtained showed generally that high concentrations of heavy metals were recorded in the central area of Gabes gulf, nearer to Gabes city; the low concentrations were in contrast found at the edges of this gulf, which is probably due to the chemical pollution generated from the huge industry of phosphoric acid in Gabes city. Comparing the results found with the four examined species, the lowest concentrations were noted with the two bivalve species P. radiata and P. nobilis. The highest heavy metals’ concentrations, noted during this study, are comparable to the findings of other authors in other areas, but they are considerably beyond the standards. A remediation action is necessarily needed either by reducing the amount of heavy metals in the phosphgypums quantities thrown directly in the sea or by stopping completely this harmful industry to protect the marine life in the area. A remediation action is necessarily needed to protect the marine life in the area.

Biology and distribution of chafers (Coleoptera, Scarabaeidae) living in hollow trees in Sweden.

OpenAIRE

Nilsson, Sven; Baranowski, Rickard; Hedin, Jonas; Jansson, Niklas; Ranius, Thomas

2002-01-01

We review the ecology and distributions of the chafers Liocola marmorata (F.), Gnorimus nobilis (L.) and Gnorimus variabilis (L.) in Sweden based on museum and several large private collections. These species live in hollow deciduous trees, in Sweden especially in oaks. The former and recently documented localities are shown on maps. More than 100 years ago, all the species as well as their habitats were more common in Sweden than today. One problem when interpreting old finds is that hollow ...

Essential oils and anxiolytic aromatherapy.

Science.gov (United States)

Setzer, William N

2009-09-01

A number of essential oils are currently in use as aromatherapy agents to relieve anxiety, stress, and depression. Popular anxiolytic oils include lavender (Lavandula angustifolia), rose (Rosa damascena), orange (Citrus sinensis), bergamot (Citrus aurantium), lemon (Citrus limon), sandalwood (Santalum album), clary sage (Salvia sclarea), Roman chamomile (Anthemis nobilis), and rose-scented geranium (Pelargonium spp.). This review discusses the chemical constituents and CNS effects of these aromatherapeutic essential oils, as well as recent studies on additional essential oils with anxiolytic activities.

29 CFR 780.114 - Wild commodities.

Science.gov (United States)

2010-07-01

... Agricultural Or Horticultural Commodities § 780.114 Wild commodities. Employees engaged in the gathering or harvesting of wild commodities such as mosses, wild rice, burls and laurel plants, the trapping of wild... 29 Labor 3 2010-07-01 2010-07-01 false Wild commodities. 780.114 Section 780.114 Labor Regulations...

Forecasting the future of coast live oak forests in the face of sudden oak death

Science.gov (United States)

Letty B. Brown; Barbara Allen-Diaz

2006-01-01

Little is known about the potential short- and long-term impacts of sudden oak death (SOD) on forest structure and composition. This study began in 2002 to evaluate the effects of SOD on coast live oak (Quercus agrifolia) - California bay laurel (Umbellularia californica) forests over a gradient of Phytophthora ramorum...

Spatial variation in effects of temperature on Phenotypic characteristics of Phytophthora ramorum isolates from eastern Sonoma county

Science.gov (United States)

Valerie Sherron; Nathan E. Rank; Michael Cohen; Brian L. Anacker; Ross K. Meentemeyer

2008-01-01

Quantifying the growth rates of plant pathogens in the laboratory can be useful for predicting rates of disease spread and impact in nature. The purpose of this study was to examine phenotypic variation among isolates of Phytophthora ramorum collected from a foliar host plant species, Umbellularia californica (California bay laurel...

Bird communities in two oceanic island forests fragmented by roads ...

African Journals Online (AJOL)

Although most studies on road effects on birds have been conducted on continental grounds, road fragmentation on oceanic islands is often heavier. We assessed variation in bird communities near (≤ 25 m) and far (>100 m) from forest roads dividing laurel and pine forests on Tenerife, Canary Islands. Line transects were ...

First report of Phytophthora ramorum infecting grand fir in California

Science.gov (United States)

K.L. Riley; G.A. Chastagner

2011-01-01

Phytophthora ramorum was detected on grand fir in 2003 and 2005 in a Christmas tree plantation near Los Gatos, CA, in association with infected California bay laurel. Isolates derived from stem lesions were used to inoculate grand fir seedlings in two tests. Isolations from lesions on inoculated plants were positive for P. ramorum...

"No Rugby--No Fear": Collective Stories, Masculinities and Transformative Possibilities in Schools

Science.gov (United States)

Pringle, Richard

2008-01-01

This paper contributes to the development of a critical pedagogy in physical education (PE) by illustrating how "collective stories" can be used within schools to help raise awareness of the relationships between sport, PE and gendered identities. A collective story, a concept developed by Laurel Richardson, aims to give voice to those…

SAMJ IZINDABA.indd

African Journals Online (AJOL)

laurels to rest on', several viral haemorrhagic fever (VHF) cases or 'outbreaks' had been successfully contained over the past decade and a half. The Izindaba report was based on opinions expressed by former CDC staffer and now local disease surveillance expert Dr. Andrew Medina-Marino on his return from helping out ...

Asymbiotic seed germination and in vitro propagation of ...

African Journals Online (AJOL)

Joe Krawczyszyn

2015-10-13

Oct 13, 2015 ... Christenson. Plant Growth Regul. 65:381-387. Knudson L (1946). A new nutrient solution for the germination of orchid seed. Am. Orchid Soc. Bull. 14:214-217. Lloyd G, MCCown B (1980). Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Int. Plant. Prop.

Work of the Hand through the Curriculum and across the Planes of Development: A Compilation of Creative Ideas

Science.gov (United States)

NAMTA Journal, 2013

2013-01-01

This article and the one that follows, "Quilt-Making in the Elementary Class" (EJ1077043), emerge from Mountain Laurel Montessori School and provide examples of the intrinsic links between the hand and academic lessons. This article features a compilation of artful recipes for young children (Soap Sculpting Clay, Easy Flour Paste, Face…

STUDIES ON SOME MORPHOLOGICAL CHARACTERS IN BIGHEAD CARP FRY

Directory of Open Access Journals (Sweden)

ELENA CIORNEA

2008-10-01

Full Text Available The present paper investigates some morphological characters (total length, standard bodily length, head length, maxim height and bodily circumference in representatives of bighead carp (Aristichthys nobilis occurring in their first growing summer. Biometric analyses were performed on 100 individuals, in the end the morphological characters under investigations being statistically processed. Explanation of the experimental results evidences low values of the standard error of the mean, which is indicative of a grouping of all external bodily variables under investigation close to the average value.

Inducción y enraizamiento de brotes epicórmicos de Cordia alliodora (Ruiz Et Pavon, Oken

Directory of Open Access Journals (Sweden)

Mercedes Susana Carranza Patiño

2016-10-01

Full Text Available La atractiva apariencia de la madera de Cordia alliodora (laurel, sus características físico-mecánicas, su abundante regeneración natural, captura de carbono y protección al suelo, la hacen ideal para la reforestación. No obstante, la falta de material de siembra de buena calidad para suplir la demanda media anual, convierten la propagación vegetativa en una herramienta esencial para el mejoramiento genético, y la conservación de genotipos en bancos clonales. En este sentido, se propuso establecer una metodología para la inducción y enraizamiento de brotes epicórmicos de árboles de laurel utilizando reguladores de crecimiento vegetal. Las concentraciones de citoquininas empleadas para la inducción de brotes a partir de árboles adultos fueron de: 0, 3000, 6000, 9000 mg L-1 de BAP sola y combinadas con 1000, 2000, 3000 mg L-1 de AIA, y se determinaron utilizando un Diseño Completo Aleatorizado (DCA. Las concentraciones de auxinas para el enraizamiento de los brotes fueron de 0, 1000, y 1500 mg kg-1 de ANA y AIB, mediante un DCA con arreglo factorial 3 x 3 (hormona ANA x hormona AIB. Se evaluó la inducción de brotes epicórmicos y se obtuvieron 2.67 brotes de 16.42 cm de longitud, con 6000 mg kg-1 de BAP + 2000 mg kg-1 de AIA. El porcentaje de enraizamiento fue 54%, número de raíces 1.78, longitud 2.59 con 1500 mg kg-1 de ANA + 1500 mg kg-1 de AIB. El uso de citoquininas y auxinas fue efectivo para provocar la diferenciación celular tanto en la inducción y rizogénesis de brotes epicórmicos de laurel.

Effect of antioxidant on the oxidation stability and combustion–performance–emission characteristics of a diesel engine fueled with diesel–biodiesel blend

International Nuclear Information System (INIS)

Rashedul, H.K.; Masjuki, H.H.; Kalam, M.A.; Teoh, Y.H.; How, H.G.; Rizwanul Fattah, I.M.

2015-01-01

Highlights: • Alexandrian laurel or Calophyllum inophyllum biodiesel blend fulfill the ASTM (D7467) specification. • Addition of antioxidant to biodiesel higher the oxidation stability. • Antioxidant treated blends showed lower NO X and BSFC compared to untreated blend. • Antioxidant treated blends showed higher CO, HC and smoke compared to untreated blend. - Abstract: Alexandrian laurel or Calophyllum inophyllum oil is recently considered one of the most anticipated nonconsumable or nonedible biodiesel sources. An attempt has been made in this study to increase the oxidation stability and investigate the engine performance, emission, and combustion characteristics of a diesel engine by adding 1% (by vol.) of two antioxidants, such as 2,6-Di-tert.-butyl-4-methylphenol and 2,2′-methylenebis (4-methyl-6-tert-butylphenol), in higher percentages of C. inophyllum biodiesel (CB30) with diesel fuel (B0). The experiment was performed on a single-cylinder, water-cooled, direct-injection diesel engine for this purpose. The addition of both antioxidants increased the oxidation stability without significantly changing other physicochemical properties. Results also show that the antioxidants enhanced the start of combustion of biodiesel, which resulted in a short ignition delay. The peak pressure and the peak heat release rate during premixed combustion phase of pure CB30 and its modified blend with antioxidant were higher than those of B0. Both antioxidant blends showed higher brake power, higher brake thermal efficiency, and lower brake specific fuel consumption than pure CB30. Both antioxidants significantly reduced NO X emission; however, CO, HC, and smoke opacity were slightly higher than those of CB30. Based on this study, Alexandrian laurel or C. inophyllum biodiesel blend (CB30) with antioxidant can be used as an alternative fuel in a diesel engine without modifications.

North American Lauraceae: terpenoid emissions, relative attraction and boring preferences of redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae).

Science.gov (United States)

Paul E. Kendra; Wayne S. Montgomery; Jerome Niogret; Grechen E. Pruett; Albert (Bud) Mayfield; Martin MacKenzie; Mark A. Deyrup; Gary R. Bauchan; Randy C. Ploetz

2014-01-01

Abstract The invasive redbay ambrosia beetle, Xyleborus glabratus, is the primary vector of Raffaelea lauricola, a symbiotic fungus and the etiologic agent of laurel wilt. This lethal disease has caused severe mortality of redbay (Persea borbonia) and swampbay (P. palustris) trees in the southeastern USA, threatens avocado (P. americana) production in Florida, and has...

Are Shaman Paintings Material Religion or Religious Art?

Directory of Open Access Journals (Sweden)

Jong-chun Park

2017-12-01

Full Text Available Laurel Kendall, Jongsung Yang, and Yul Soo Yoon. God Pictures in Korean Contexts: The Ownership and Meaning of Shaman Paintings. Honolulu: University of Hawai'i Press, 2015. God Pictures in Korean Contexts: The Ownership and Meaning of Shaman Paintings is a pioneering work, one that explores the proverbial “road not taken” by previous research on the subject. The authors situate “the lives of Korean shaman paintings [musindo] in a complex South Korean world; in shaman shrines, private collections, and museums." Incorporating their vivid experiences in the field, authors Laurel Kendall, anthropologist and curator at the American Museum of Natural History; Jongsung Yang, collector and director of the Museum of Shamanism in Seoul; and Yul Soo Yoon, art historian and director of the Gahoe Museum in Seoul, investigate not merely the religious meanings and functions of shaman paintings, but how these meanings and functions are accepted, appropriated, and even created, depending on the interests of various participants—shamans, painters, collectors of shaman paintings, and so on—who are relevant to these visual forms of expressive culture.

Chemical composition and antifungal activity of essential oils from medicinal plants of Kazakhstan.

Science.gov (United States)

Sampietro, Diego A; Gomez, Analía de Los A; Jimenez, Cristina M; Lizarraga, Emilio F; Ibatayev, Zharkyn A; Suleimen, Yerlan M; Catalán, Cesar A

2017-06-01

The composition of essential oils from leaves of Kazakhstan medicinal plants was analysed by GC-MS. The major compounds identified were 1,8-cineole (34.2%), myrcene (19.1%) and α-pinene (9.4%) in Ajania fruticulosa; 1,8-cineole (21.0%), β-thujone (11.0%), camphor (8.5%), borneol (7.3%) and α-thujone (6.5%), in Achillea nobilis; camphor (47.3%), 1,8-cineole (23.9%), camphene (9.8%) and β-thujone (6.0%) in Artemisia terrae-albae; 1,8-cineole(55.8%) and β-pinene (6.2%) in Hyssopus ambiguus; α-thuyene(46.3%) and δ-cadinene(6.3%) in Juniperus sibirica; sabinene (64%) in Juniperus sabina; and α-pinene (51.5%), β-phellandrene (11.2%) and δ-cadinene (6.3%) in Pinus sibirica. The essential oils did not show antifungal effect (MIC > 1.20 mg/mL) on Aspergillus carbonarius and Aspergillus niger, while the oils from A. nobilis, A. terrae-albae, H. ambiguus and J. sabina exhibited moderate and moderate to weak antimicrobial activities on Fusarium verticillioides (MIC = 0.60 mg/mL) and Fusarium graminearum (MIC = 0.60-1.20 mg/mL), respectively. A principal component analysis associated the antifungal activity (r 2  > 0.80, p = 0.05) with the presence of borneol, camphor, camphene, 1,8-cineole,α- and β-thujone, and of the oxygenated monoterpenes.

Effect of tree species and end seal on attractiveness and utility of cut bolts to the redbay Ambrosia beetle and granulate Ambrosia beetle (Coleoptera: Curculionidae: Scolytinae)

Science.gov (United States)

Albert E. Mayfield; James L. Hanula

2012-01-01

The redbay ambrosia beetle, Xyleborus glabratus Eichhoff, is a non-native invasive pest and vector of the fungus that causes laurel wilt disease in certain trees of the family Lauraceae. This study assessed the relative attractiveness and suitability of cut bolts of several tree species to X. glabratus. In 2009, female X. glabratus were equally attracted to traps...

Author Details

African Journals Online (AJOL)

Izindaba: Ebola: SA has no outbreak 'laurels' to rest on. Abstract PDF · Vol 104, No 10 (2014) - Articles Izindaba: Dismal obs/gynae training contributing to maternal deaths – Motsoaledi Abstract PDF · Vol 104, No 10 (2014) - Articles Izindaba: BHF and Government: 'I'll change if you'll change' Abstract PDF · Vol 104, No 11 ...

A Regional Guidebook for Applying the Hydrogeomorphic Approach to the Functional Assessment of Forested Wetlands in Alluvial Valleys of East Texas

Science.gov (United States)

2010-10-01

plant species as the low-gradient riverine subclass. However, species such as American elm (Ulmus americana), slippery elm (Ulmus rubra), winged elm ...americana American elm Ulmus crassifolia cedar elm Ulmus rubra slippery elm Ulmus spp. elm Viburnum nudum possumhaw Vitis rotundifolia muscadine...laurel oak (Q. laurifolia), Shumard oak (Q. shumardii), elms (Ulmus sp.), sweetgum (Liquidambar styraciflua), southern magnolia (Magnolia

Sélection de parutions

Directory of Open Access Journals (Sweden)

2006-12-01

Full Text Available Si vous souhaitez soumettre une analyse d'ouvrage concernant le domaine des TIC et des langues, contactez-moi : foucher@lrl.univ-bpclermont.fr Paola NobiliOltre il libro di testo. Multimedialità e nuovi contesti per apprendere le lingueRoma : Carocci Faber, 2006ISBN : 88-7766-287-416,50 € Nicolas GuichonLangues et TICE - Méthodologie de conception multimédiaParis : Ophrys, collection Autoformation et Enseignement Multimédia, 2006ISBN : 2-7080-1155-3236 pages22 € Benoît HabertInstruments et re...

Antiproliferative effects of the readily extractable fractions prepared from various citrus juices on several cancer cell lines.

Science.gov (United States)

Kawaii, S; Tomono, Y; Katase, E; Ogawa, K; Yano, M

1999-07-01

To eliminate the masking effect by flavonoid glycosides, which comprise approximately 70% of conventionally prepared sample, the readily extractable fraction from Citrus juice, which was prepared by adsorbing on HP-20 resin and eluting with ethanol and acetone from the resin, was subjected to antiproliferative tests against several cancer cell lines. Screening of 34 Citrus juices indicated that King (Citrus nobilis) strongly inhibited proliferation of all cancer cell lines examined. Sweet lime and Kabuchi inhibited three of the four cancer cell lines. In contrast, these samples were substantially less cytotoxic toward normal human cell lines.

Directory of Open Access Journals (Sweden)

Juan Valladolid Ontaneda

2017-06-01

Full Text Available El estudio de selección de árboles semilleros se realizó en las comunas Olón, Dos Mangas y Salanguillo del cantón Santa Elena, provincia de Santa Elena, Ecuador. El objetivo del estudio fue, identificar, caracterizar y seleccionar árboles semilleros de especies maderables con buenas características morfológicas, y que permitan disponer de semillas para trabajos de propagación de especies forestales en viveros con fines de reforestación. El estudio se inicia con la identificación y ubicación de plantaciones forestales de varias especies forestales establecidas entre los años 2000 a 2005, en las plantaciones se evaluaron las características dasométricas y morfológicas, las que permitieron seleccionar 11 árboles con características morfológicas deseables correspondientes a cinco especies forestales laurel blanco(Cordia alliodora, laurel negro(Cordia megalantha, caoba (Swietenia macrophylla, cedro (Cedrela odorata y melina (Gmelina arbórea; cada árbol fue georreferenciado utilizando coordenadas UTM Datum WGS 1984 Zona 17M. La cantidad de semillas que pueden producir dichas especies forestales es proyectada por año la cual servirá como referencia para establecer programas de reforestación con materiales propios y adaptados a las condiciones ambientales en la provincia de Santa Elena Abstract  The study of selection of seed trees was carried out in the Olón, Dos Mangas and Salanguillo communes of the canton Santa Elena, Santa Elena province, Ecuador. The objective of the study was to identify, characterize and select seedlings of timber species with good morphological characteristics, and to allow seeds to be used for the propagation of forest species in nurseries for reforestation purposes. The study began with the identification and location of forest plantations of several forest species established between 2000 and 2005, on the plantations, the dasometric and morphological characteristics were evaluated, allowing the

Computalog; no resting on laurels for this company

International Nuclear Information System (INIS)

Jaremko, G.

1997-01-01

Computalog, one of the Canadian oilfield service sector's strongest survivors, is celebrating its 25th anniversary in 1997. In an interview with 'Oilweek' the company's president confidently predicted that in the not-too-distant future, the company will derive at least half of its revenues from foreign sources. Considerable progress has already been made towards this goal: in 1996 some 30 per cent of revenues have been derived from operations in the United States, Russia, India, China, Argentina and Venezuela. Currently, the company has 1023 employees (80 in Calgary), and dominates the Canadian market for 'wireline services' of recording or logging features of wells with increasingly elaborate, computerized electronic instruments. In addition to its core business, the company is active in directional and horizontal drilling, product sales, manufacturing, and research and development. Although majority Canadian-owned, and perceived to be a primarily Canadian company, its current goal is to gain greater exposure on the global market and to have a larger following on Wall Street

Cost Model/Data Base Catalog Non-DoD/Academic Survey. Volume 1. Project Summary

Science.gov (United States)

1988-10-30

and to schedule briefings, i I IV-ii. I I • Applied Physics Laboratory i Bureau of Economic Analysis, Department of Commerce * Congressional Budget... Physics Laboratory Mr. Edward M. Portner Assistant Director, Business Operations Applied Physics Laboratory, Room 7-248 Johns Hopkins Road Laurel, MD...College) Defense Systems Management Collge Director, PMSS Directorate Ft. Belvoir, VA 22060-5426 1 33. Decision-Science Applications, Inc. Decision

Combining Surveillance Systems: Effective Merging of U.S. Veteran and Military Health Data

Science.gov (United States)

2016-08-04

Department of Defense, Silver Spring, Maryland, United States of America, 2 Applied Physics Laboratory, Johns Hopkins University, Laurel, Maryland...was used to examine seasonal influenza trends, a gastrointestinal (GI) illness epidemic and a heat-related event. This provided the first example of the... geriatric clinics, women’s primary care, infectious diseases and emergency medicine. We grouped visits into an influenza-like-illness (ILI) syndrome

DNA-based confirmation that the parasitic wasp Cotesia glomerata (Braconidae, Hymenoptera) is a new threat to endemic butterflies of the Canary Islands

Czech Academy of Sciences Publication Activity Database

Lozan, Aurel; Monaghan, M. T.; Spitzer, Karel; Jaroš, Josef; Žurovcová, Martina; Brož, V.

2008-01-01

Roč. 9, č. 6 (2008), s. 1431-1437 ISSN 1566-0621 R&D Projects: GA AV ČR 1QS500070505 Grant - others:EU SYNTHESIS(BE) GB-TAF-2063; UK Biotechnology and Biological Sciences Research Council(GB) BBS/B/04358 Institutional research plan: CEZ:AV0Z50070508 Keywords : alien parasitoids * threatened butterflies * laurel forests Subject RIV: EH - Ecology, Behaviour Impact factor: 2.408, year: 2008

Early Training Estimation System

Science.gov (United States)

1980-08-01

first year efforts in CTES development were Cecil Wakelin, Gavin Livingstone, Ray Walsh« Peter Weddle, David Herlihy, Laurel Brown, Drs. Paul Ronco...and Society, 1980, pp. 1067-1974. David , J., Price, J. Successful communication in full scale engineering development statements of work. Air Force...1980, U.S. Army Engineering Laboratory. Shrier , S. Algorithms for system design. Proceedings of the International Conference on Cybernetics and

Cycles of selected elements in the frame of Globalization and Global Change in the environment of Tenerife (Canary Islands, Spain)

Science.gov (United States)

Heidak, Markus O.; Glasmacher, Ulrich A.; Schöler, Heinfried; Trieloff, Mario; Kober, Bernd

2010-05-01

The Laurel Forest is an important and sensitive ecosystem with particular element cycling mechanisms. On Tenerife the distribution is straitened to some parts in the north, north-west and northeast. The NE trade wind ensures a permanently humid climate in the north. Major urban and industrial development is centred on Tenerife, and as a touristy hotspot the Island is exposed to heavy air traffic. Furthermore, the short distance to the African coastline and, therefore, to the Sahara, contribute a regular influence of African Dust emissions. In summary, Laurel Forest is exposed to different climatic conditions, variations in lithology and soils, and aerosols caused by local anthropogenic emissions, Saharan dust, and sea spray. The present study aims to understand geogenic and anthropogenic element transports of K, P, N, and organic components between soils and Laurel Forest. In addition, the element contribution from the aerosols such as the Sahara dust has to be quantified to understand the rock - soil - vegetation coupling system. The Sahara dust as one of the important aerosols has been studied by various researchers (Bustos et al., 1998; Rodrıguez, 1999; Torres et al., 2001; Viana et al., 2002). Viana et al.,(2002) quantified the impacts of African dust outbreaks for Tenerife and Gran Canaria, after the interpretation of the PM10 (thoracis particulate matter) from nineteen air quality monitoring stations. Three types of African dust contributions were identified and characterized (winter, summer and autumn-winter dust outbreaks). Collected samples with and without African dust influence proved that: (a) for the intensive winter African dust outbreaks (daily PM10 levels up to 191 mg/m3) at least 76% of the bulk PM10 levels may be attributable to dust load, whereas the anthropogenic input accounts for only 3-14% and (b) SiO2, Al2O3, Ca, K, Fe, Ti, V, Mn and Ba concentrations are excellent tracers of African origin (Viana et al., 2002).

Sea Cucumber Resources At Tanjung Pai Waters Padaido Biak Numfor Papua

Directory of Open Access Journals (Sweden)

Eddy Yusron

2004-12-01

Full Text Available Observation on sea cucumber diversity was carried out at coastal waters of Pai and Imbeyomi Islands in the Padaido Island Biak Numfor. Sampling was done by using a transect quadrant of 1 m x 1 m. This sampling and observation on its microhabitat were conducted by snorkling. Analyses on the sea cucumber community structure were based on its frequency of occurance, diversity, and density. The results showed that at both locations 10 species of sea cucumber were found where Holothuria edulis, H. atra, and H. nobilis were predominant common and more evenly distributed than the other species.

Confocal microscopy as a useful approach to describe gill rakers of Asian species of carp and native filter-feeding fishes of the upper Mississippi River system

Science.gov (United States)

Liza R. Walleser,; D.R. Howard,; Sandheinrich, Mark B.; Gaikowski, Mark P.; Amberg, Jon J.

2014-01-01

To better understand potential diet overlap among exotic Asian species of carp and native species of filter-feeding fishes of the upper Mississippi River system, microscopy was used to document morphological differences in the gill rakers. Analysing samples first with light microscopy and subsequently with confocal microscopy, the three-dimensional structure of gill rakers in Hypophthalmichthys molitrix,Hypophthalmichthys nobilis and Dorosoma cepedianum was more thoroughly described and illustrated than previous work with traditional microscopy techniques. The three-dimensional structure of gill rakers in Ictiobus cyprinellus was described and illustrated for the first time.

Assessment of dreissenid biodeposits as a potential food resource for invasive Asian carp

Science.gov (United States)

Anderson, Karl R.; Chapman, Duane C.; Hayer, Cari-Ann

2016-01-01

Silver carp (Hypophthalmichthys molitrix) and bighead carp (H. nobilis) are poised to invade the Laurentian Great Lakes. Zebra mussels (Dreissena polymorpha) and quagga mussels (D. rostriformis bugensis) have shifted nutrient pathways towards the benthos, partly through deposition of feces and rejected food particles called biodeposits. When biodeposit material was fed to bighead and silver carp, they fed on the material, but on average lost weight. Energy density between fed and unfed fish did not differ, but a few individual fish did gain weight on the biodeposits diet. Our results demonstrate that biodeposits might be considered a supplemental food for bigheaded carps.

Relationship between structure and antiproliferative activity of polymethoxyflavones towards HL60 cells.

Science.gov (United States)

Kawaii, Satoru; Ikuina, Tomoyasu; Hikima, Takeshi; Tokiwano, Tetsuo; Yoshizawa, Yuko

2012-12-01

As part of our continuing investigation of polymethoxyflavone (PMF) derivatives as potential anticancer substances, a series of PMF derivatives was synthesized. The synthesized compounds were evaluated for cytotoxicity against the promyelocytic leukemic HL60 cell line, and structure-activity relationship correlations were investigated along with previously isolated PMFs from the peel of king orange (Citrus nobilis). 7,3'-Dimethoxyflavone demonstrated the most potent activity among the synthetic PMFs. Consideration of correlation between the methoxylation pattern and antiproliferative activity revealed the importance of the 3'-methoxyl group and the higher degree of methoxylation on the A-ring moiety of PMFs.

ÎNCADRAREA ŞLEAHTEI POLONEZE ÎN RÂNDURILE NOBILIMII DIN BASARABIA PE PARCURSUL SECOLULUI Al XIX-lea (Cazul familiilor de nobili Celinski şi Cernilev/Cernelevski

Directory of Open Access Journals (Sweden)

Cristina GHERASIM

2015-11-01

Full Text Available În articolul dat, în baza documentelor de arhivă inedite şi a studiilor monografice publicate, vom analiza problema privind încadrarea nobilimii alogene în rândurile nobilimii din Basarabia, având ca exemplu şleahta poloneză. Basarabia, fiind amplasată strategic, a prezentat un interes deosebit în politica de extindere a Imperiului Rus. Din acest considerent, ţarismul, odată cu anexarea acestui teritoriu, a căutat să-şi creeze aici o baza socială care să susţină şi să promoveze politica imperială. Pe lângă acordarea diferitelor privilegii populaţiei autohtone, în special boierimii, administraţia imperială a promovat o politică de colonizare a teritoriului dintre Prut şi Nistru şi de infiltrare în rândul boierimii autohtone a nobililor sau simplilor funcţionari de origine rusă, precum şi unora veniţi din Austria, Polonia etc. Ca rezultat, pe parcursul sec. al XIX-lea, alături de boierii autohtoni, în procesul de confirmare a titlurilor nobiliare la Comisia instituită în acest sens s-au încadrat şi reprezentanţi ai nobilimii alogene. Analizând dosarele depuse de şleahta poloneză, observăm că reprezentanţilor acesteia li se confirma titlul nobiliar în urma prezentării unei adeverinţe care certifica că Adunarea Deputaţilor Nobilimii din Podolia deja recunoscuse apartenenţa nobiliară a solicitantului. Însă, depistăm şi cazuri când reprezentanţilor şleahtei poloneze, după mai multe încercări, nu li se confirma titlul nobiliar, cu toate că Adunarea Deputaţilor din Podolia îi recunoscuse deja în rândul nobilimii. Din această categorie face parte, spre exemplu, familia de nobili Cernelevski, membrilor căreia la 18 decembrie 1802 Adunarea Deputaţilor din Podolia le-a confirmat titlul nobiliar, ca urmaşi ai boierului moldovean, paharnicului Andronache Cernelevski. Însă, urmaşilor săi le-au fost respinse toate cererile depuse, din considerentul că din dosarul analizat de c

MARINATED WITH EFFECT OF NaCl AND SODIUM TRIPOLYPHOSPHATE BROMATOLOGICAL PROPERTIES ON THE MEAT GOAT

OpenAIRE

Ricardo Peña F.; Daniel Salvador Duran O; Luis Carlos Baleta M.

2014-01-01

Goat meat has all the significant nutritional characteristics by consumers to be an excellent alternative supply; therefore, this study proposes the use of a mixture of NaCl, sodium tripolyphosphate, laurel and rosemary extracts as marginalisers agents using maceration technique marinade as facilitator system and applying vacuum packaging and storage at refrigeration of an overall strategy of modernization. In the finished product bromatologic variables pH, fat, protein, water holding c...

Regional Guidebook for Applying the Hydrogeomorphic Approach to Assessing the Functions of Headwater Slope Wetlands on the South Carolina Coastal Plain

Science.gov (United States)

2011-09-01

i.e., swamp bay) ( Persea borbonia) in the overstory or midstory ERDC/EL TR-11-11 20 (Monk 1966, Nelson 1986, Wharton et al. 1977, USACE 1988). In...biflora swamp tupelo Persea borbonia redbay Pinus glabra spruce pine Pinus taeda loblolly pine Quercus laurifolia laurel oak Quercus michauxii swamp... American elm Group 22 Carpinus caroliniana American hornbeam 0.66 Celtis laevigata sugarberry Cornus foemina stiff dogwood Diospyros virginiana

Civilian Reuse of Former Military Bases. Summary of Completed Military Base Economic Adjustment Projects

Science.gov (United States)

1990-06-01

Theodore Army Terminal 1965 (-) Unde, deal Basic Industries, Chamber of Commerce . P.O. Box 2187 Mobile Paint Mfg Co, Hula, Taylor- Mobile. AL 36652 Wharton...585 650 Sidney Warehousing Activities, 300(C) Anita Pennel, Chamber of Commerce . Sioux Army Depot 1967 (2) Western Nebraska Community College, Sidney...Inc. Laurel Oaks Vo-Tech, 600(S) Wilmington Area Chamber of Commerce Industrial Park, Hydro-Lift Trucks, 69 North South Street Southern State Community

Laurel wilt in avocado: Review of an emerging disease

Science.gov (United States)

aurel wilt, caused by the vascular fungus Raffaelea lauricola, is transmitted by the redbay ambrosia beetle, Xyleborus glabratus, and affects many plants in the family Lauraceae. It was introduced into the United States around 2002 through infested packing material arriving in Georgia. In Florida, t...

CALS Test Network Handbook

Science.gov (United States)

1993-03-01

Ltd Gillette Company Dorset, England Boston MA Foreign Broadcast Information Set. Giordano Associates Inc Frederick MD Long Branch NJ Formtek Inc...Associates Orem UT College Park MD InfoDesign Corp Johns Hopkins University Toronto, Ontario Laurel MD Information Spectrum Inc Joint Committee on Printing...Inc Washington DC Bel Air MD Navy Naval Research Laboratory OutSource Inc Washington DC Los Angeles CA 22 i +’ . S.. . -- -,•w• -- w,,Wmwmm mm -- m mmm

A Wave Theory for Non-Imaging Concentrators

Science.gov (United States)

1992-04-01

the radiance function over the z = 0 plane, as defined by Walther (W), 6 W. T. Welford and R. Winston, High Collection Nonimaging Optics , Academic...Laurel, Maryland 2072&-6099 REFERENCES IW. T. Welford and R. Winston, High Collection Nonimaging Optics , Academic Press, New York (1989). 2 W. Welford and...stationary optical system. For light from a quasi-homogeneous source, this expression can be somewhat simplified by an approximatior. It is shown that the

Lipid nanocarriers based on natural oils with high activity against oxygen free radicals and tumor cell proliferation

International Nuclear Information System (INIS)

Lacatusu, I.; Badea, N.; Badea, G.; Oprea, O.; Mihaila, M.A.; Kaya, D.A.; Stan, R.; Meghea, A.

2015-01-01

The development of nano-dosage forms of phytochemicals represents a significant progress of the scientific approach in the biomedical research. The aim of this study was to assess the effectiveness of lipid nanocarriers based on natural oils (grape seed oil, fish oil and laurel leaf oil) in counteracting free radicals and combating certain tumor cells. No drug was encapsulated in the nanocarriers. The cytotoxic effect exerted by bioactive nanocarriers against two tumor cells, MDA-MB 231 and HeLa cell lines, and two normal cells, L929 and B16 cell lines, was measured using the MTT assay, while oxidative damage was assessed by measuring the total antioxidant activity using chemiluminescence analysis. The best performance was obtained for nanocarriers based on an association of grape seed and laurel leaf oils, with a capacity to scavenge about 98% oxygen free radicals. A dose of nanocarriers of 5 mg·mL −1 has led to a drastic decrease in tumor cell proliferation even in the absence of an antitumor drug (e.g. about 50% viability for MDA-MB 231 cell line and 60% viability for HeLa cell line). A comparative survival profile of normal and tumor cells, which were exposed to an effective dose of 2.5 mg·mL −1 lipid nanocarriers, has revealed a death rate of 20% for normal B16 cells and of 40% death rate for MDA-MB 231 and HeLa tumor cells. The results in this study imply that lipid nanocarriers based on grape seed oil in association with laurel leaf oil could be a candidate to reduce the delivery system toxicity and may significantly improve the therapeutic efficacy of antitumor drugs in clinical applications. - Highlights: • Functional lipid nanocarriers with unique features and broad spectrum effectiveness • Lipid nanocarriers based on laureal leaf oil (LLO) and grape seed oil (GSO) • Antioxidant activity has reached 98% for nanocarriers containing 25% GSO and 2% LLO. • LLO exerts a significant cytotoxic effect against HeLa and MDA-MB 231 tumor cells. • 50

Lipid nanocarriers based on natural oils with high activity against oxygen free radicals and tumor cell proliferation

Energy Technology Data Exchange (ETDEWEB)

Lacatusu, I.; Badea, N.; Badea, G.; Oprea, O. [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No 1, 011061 Bucharest (Romania); Mihaila, M.A. [Institute of Virusology “Stefan S. Nicolau”, Center of Immunology, Bravu Road, No. 285, 030304 Bucharest (Romania); Kaya, D.A. [Department of Field Crops, Faculty of Agriculture, Mustafa Kemal University, 31030 Antakya, Hatay (Turkey); Stan, R., E-mail: rl_stan2000@yahoo.com [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No 1, 011061 Bucharest (Romania); Meghea, A. [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No 1, 011061 Bucharest (Romania)

2015-11-01

The development of nano-dosage forms of phytochemicals represents a significant progress of the scientific approach in the biomedical research. The aim of this study was to assess the effectiveness of lipid nanocarriers based on natural oils (grape seed oil, fish oil and laurel leaf oil) in counteracting free radicals and combating certain tumor cells. No drug was encapsulated in the nanocarriers. The cytotoxic effect exerted by bioactive nanocarriers against two tumor cells, MDA-MB 231 and HeLa cell lines, and two normal cells, L929 and B16 cell lines, was measured using the MTT assay, while oxidative damage was assessed by measuring the total antioxidant activity using chemiluminescence analysis. The best performance was obtained for nanocarriers based on an association of grape seed and laurel leaf oils, with a capacity to scavenge about 98% oxygen free radicals. A dose of nanocarriers of 5 mg·mL{sup −1} has led to a drastic decrease in tumor cell proliferation even in the absence of an antitumor drug (e.g. about 50% viability for MDA-MB 231 cell line and 60% viability for HeLa cell line). A comparative survival profile of normal and tumor cells, which were exposed to an effective dose of 2.5 mg·mL{sup −1} lipid nanocarriers, has revealed a death rate of 20% for normal B16 cells and of 40% death rate for MDA-MB 231 and HeLa tumor cells. The results in this study imply that lipid nanocarriers based on grape seed oil in association with laurel leaf oil could be a candidate to reduce the delivery system toxicity and may significantly improve the therapeutic efficacy of antitumor drugs in clinical applications. - Highlights: • Functional lipid nanocarriers with unique features and broad spectrum effectiveness • Lipid nanocarriers based on laureal leaf oil (LLO) and grape seed oil (GSO) • Antioxidant activity has reached 98% for nanocarriers containing 25% GSO and 2% LLO. • LLO exerts a significant cytotoxic effect against HeLa and MDA-MB 231 tumor

From One Leader to Another

Science.gov (United States)

2013-01-01

like a “Laurel and Hardy movie ” she was hit on the head by a 4x4 board that rolled off the side of the 5-ton trailer . Later that afternoon the Soldier... Reception and Integration Activities, Functional Training and talent management within an organization are all semi-formal processes (items that may not...stage which involves the Reception and Integration of Soldiers to the unit. During this stage, the flow of communication is essential for the

The imperative of culture: a quantitative analysis of the impact of culture on workforce engagement, patient experience, physician engagement, value-based purchasing, and turnover

OpenAIRE

Owens, Katie; Eggers, Jim; Keller, Stephanie; McDonald, Audrey

2017-01-01

Katie Owens,1 Jim Eggers,2 Stephanie Keller,1 Audrey McDonald1 1HealthStream Engagement Institute, Pensacola, FL, 2Analytics, HealthStream, Laurel, MD, USA Abstract: Current uncertainty for the future of the health care landscape is placing an increasing amount of pressure on leadership teams to be prepared to steer their organization forward in a number of potential directions. It is commonly recognized among health care leaders that culture will either enable or disable organizational succe...

Old Books Bring New Life to the Brick and Mortar Library

Science.gov (United States)

2012-08-01

Beijing 108   constellations, centering primarily on the Greek and Roman mythology surrounding the constellations. As with star atlases that would...obscure Greek myths. So who was Hydra and why is he always shown with a crow and a cup in a laurel tree? The Greek myth says Apollo asked the crow to... Greek letters to the brighter starts, with alpha being the brightest. These letters were placed on the charts themselves. So just a few years before

Topical Formulation Comprising Fatty Acid Extract from Cod Liver Oil: Development, Evaluation and Stability Studies.

Science.gov (United States)

Ilievska, Biljana; Loftsson, Thorsteinn; Hjalmarsdottir, Martha Asdis; Asgrimsdottir, Gudrun Marta

2016-06-01

The purpose of this study was to develop a pharmaceutical formulation containing fatty acid extract rich in free omega-3 fatty acids such as eicosapentaenoic acid and docosahexaenoic acid for topical use. Although the health benefits of cod liver oil and other fish oils taken orally as a dietary supplement have been acknowledged and exploited, it is clear that their use can be extended further to cover their antibacterial properties. In vitro evaluation showed that 20% (v/v) fatty acid extract exhibits good activity against strains of the Gram-positive bacteria Staphylococcus aureus, Enterococcus faecalis, Streptoccoccus pyogenes and Streptoccoccus pneumonia. Therefore, free polyunsaturated fatty acids from cod liver oil or other fish oils can be used as safe and natural antibacterial agents. In this study, ointment compositions containing free fatty acids as active antibacterial agents were prepared by using various natural waxes and characterized. The effects of different waxes, such as carnauba wax, ozokerite wax, laurel wax, beeswax, rice bran wax, candelilla wax and microcrystalline wax, in the concentration range of 1% to 5% (w/w) on the ointment texture, consistency and stability were evaluated. The results showed significant variations in texture, sensory and rheological profiles. This was attributed to the wax's nature and chain composition. Microcrystalline wax gave the best results but laurel wax, beeswax and rice bran wax exhibited excellent texturing, similar sensory profiles and well-balanced rheological properties.

Mortality and community changes drive sudden oak death impacts on litterfall and soil nitrogen cycling.

Science.gov (United States)

Cobb, Richard C; Eviner, Valerie T; Rizzo, David M

2013-10-01

Few studies have quantified pathogen impacts to ecosystem processes, despite the fact that pathogens cause or contribute to regional-scale tree mortality. We measured litterfall mass, litterfall chemistry, and soil nitrogen (N) cycling associated with multiple hosts along a gradient of mortality caused by Phytophthora ramorum, the cause of sudden oak death. In redwood forests, the epidemiological and ecological characteristics of the major overstory species determine disease patterns and the magnitude and nature of ecosystem change. Bay laurel (Umbellularia californica) has high litterfall N (0.992%), greater soil extractable NO3 -N, and transmits infection without suffering mortality. Tanoak (Notholithocarpus densiflorus) has moderate litterfall N (0.723%) and transmits infection while suffering extensive mortality that leads to higher extractable soil NO3 -N. Redwood (Sequoia sempervirens) has relatively low litterfall N (0.519%), does not suffer mortality or transmit the pathogen, but dominates forest biomass. The strongest impact of pathogen-caused mortality was the potential shift in species composition, which will alter litterfall chemistry, patterns and dynamics of litterfall mass, and increase soil NO3 -N availability. Patterns of P. ramorum spread and consequent mortality are closely associated with bay laurel abundances, suggesting this species will drive both disease emergence and subsequent ecosystem function. © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.

Juvenile Pen Shells (Pinna nobilis) Tolerate Acidification but Are Vulnerable to Warming

KAUST Repository

Basso, Lorena; Hendriks, Iris E.; Duarte, Carlos M.

2015-01-01

effects of both stressors on marine invertebrates, particularly on early life stages considered more vulnerable. Disparate responses of their singular and combined effects were reported, highlighting the importance of extending the studies to different

Are Shaman Paintings Material Religion or Religious Art?

OpenAIRE

Jong-chun Park

2017-01-01

Laurel Kendall, Jongsung Yang, and Yul Soo Yoon. God Pictures in Korean Contexts: The Ownership and Meaning of Shaman Paintings. Honolulu: University of Hawai'i Press, 2015. God Pictures in Korean Contexts: The Ownership and Meaning of Shaman Paintings is a pioneering work, one that explores the proverbial “road not taken” by previous research on the subject. The authors situate “the lives of Korean shaman paintings [musindo] in a complex South Korean world; in shaman shrines, private co...

Approaching Stan Laurel's illusion: the self-induced rubber hand phenomenon.

Science.gov (United States)

Neuf, Hartmut; Hamburger, Kai

2013-01-01

The classical rubber hand illusion is induced by an experimenter (eg stimulation with a brush) and usually realized with some sort of visual occlusion. Here, we demonstrate a new phenomenon: the self-induced rubber hand illusion. It is possible to elicit the feeling of a third hand without any help from an experimenter and under conditions of no occlusion. The findings are discussed within the context of neural plasticity.

User interface design of electronic appliances

CERN Document Server

Baumann, Konrad

2002-01-01

Foreword by Brenda Laurel. Part One: Introduction 1. Background, Bruce Thomas 2. Introduction, Konrad Baumann 3. The Interaction Design Process, Georg Rakers Part Two: User Interface Design 4. Creativity Techniques, Irene Mavrommati 5. Design Principals, Irene Mavrommati and Adrian Martel 6. Design of On-Screen Interfaces, Irene Mavrommati Part Three: Input Devices 7. Controls, Konrad Baumann 8. Keyboards, Konrad Baumann 9. Advanced Interaction Techniques, Christopher Baber and Konrad Baumann 10. Speech Control, Christopher Baber and Jan Noyes 11. Wearable Computers, Christopher Baber Part Fou

El alcanfor

OpenAIRE

García Paredes, Antonio

2012-01-01

El alcanfor es un cuerpo natural, pertenece al reino vegetal; es blanco, brillante, semitrasparente, aromático, duro, sólido, liviano, inflamable, volátil, pulverizable al ponerle unas gotas de alcohol, soluble en el alcohol y en los aceites, insoluble en el agua, medicinal e industrial. Entre los muchos vegetales que suministra alcanfor el más importante y antiguamente conocido es el Laurel Alcanfor, árbol centenario y frondoso de la familia de las Laurineas, propio del Japón y de la Chin...

Topical Formulation Comprising Fatty Acid Extract from Cod Liver Oil: Development, Evaluation and Stability Studies

Directory of Open Access Journals (Sweden)

Biljana Ilievska

2016-06-01

Full Text Available The purpose of this study was to develop a pharmaceutical formulation containing fatty acid extract rich in free omega-3 fatty acids such as eicosapentaenoic acid and docosahexaenoic acid for topical use. Although the health benefits of cod liver oil and other fish oils taken orally as a dietary supplement have been acknowledged and exploited, it is clear that their use can be extended further to cover their antibacterial properties. In vitro evaluation showed that 20% (v/v fatty acid extract exhibits good activity against strains of the Gram-positive bacteria Staphylococcus aureus, Enterococcus faecalis, Streptoccoccus pyogenes and Streptoccoccus pneumonia. Therefore, free polyunsaturated fatty acids from cod liver oil or other fish oils can be used as safe and natural antibacterial agents. In this study, ointment compositions containing free fatty acids as active antibacterial agents were prepared by using various natural waxes and characterized. The effects of different waxes, such as carnauba wax, ozokerite wax, laurel wax, beeswax, rice bran wax, candelilla wax and microcrystalline wax, in the concentration range of 1% to 5% (w/w on the ointment texture, consistency and stability were evaluated. The results showed significant variations in texture, sensory and rheological profiles. This was attributed to the wax’s nature and chain composition. Microcrystalline wax gave the best results but laurel wax, beeswax and rice bran wax exhibited excellent texturing, similar sensory profiles and well-balanced rheological properties.

Antioxidant Activities and Oxidative Stabilities of Some Unconventional Oilseeds.

Science.gov (United States)

Uluata, Sibel; Ozdemir, Nurhayat

2012-04-01

The oils of some unconventional oilseeds (hemp, radish, terebinth, stinging nettle, laurel) were obtained by a cold-press method in which the total oil content, fatty acids, tocopherol isomers, some metal contents (Ca, Mg, Fe, Cu), antioxidant activity and oxidative stability were determined. The total oil content was determined ranging between 30.68 and 43.12%, and the oil samples had large amounts of unsaturated fatty acids, with oleic acid and linoleic acid. Of all the oils, terebinth seed oil had the highest α-tocopherol content (102.21 ± 1.01 mg/kg oil). Laurel oilseed had the highest antiradical activity in both the DPPH and ABTS assays. The peroxide value of the non-oxidized oils ranged between 0.51 and 3.73 mequiv O(2)/kg oil. The TBARS value of the non-oxidized oils ranged between 0.68 ± 0.02 and 6.43 ± 0.48 mmol MA equiv/g oil. At 110 °C, the Rancimat induction period of the oils ranged between 1.32 and 43.44 h. The infrared spectra of the samples were recorded by FTIR spectroscopy. The absorbance values of the spectrum bands were observed and it was determined that some of the chemical groups of oxidized oils caused changes in absorbance. As a result of the present research, the analyzed oils could be evaluated as an alternative to traditionally consumed vegetable oils or as additives to them.

Feeding of two Cichlidae species (Perciformes in an hypertrophic urban lake

Directory of Open Access Journals (Sweden)

Yafe Alejandro

2002-01-01

Full Text Available Diet of two cichlid species, Cichlasoma facetum (Jenyns, 1842, and Gymnogeophagus rhabdotus Hensel, 1870, was studied in Rodó Lake, an urban hypertrophic lake in Uruguay. The stomach contents from 192 individuals of C. facetum and 202 of G. rhabdotus, obtained through seasonal sampling in the year 2000, were analyzed. The occurrence frequency and the alimentary importance index of each food item were calculated for each season and size class in both species. Cichlasoma facetum fed upon insects (mainly chironomid larvae and pupae, fish (Cnesterodon decemmaculatus Jenyns, 1842, and vegetals (algae, periphyton and macrophytes debris; large individuals also fed upon the freshwater shrimp Palaemonetes argentinus Nobili, 1901. Gymnogeophagus rhabdotus consumed zooplankton (mainly copepods, vegetals (algae and detritus and Chironomidae larvae in a lesser extent.

Groundwater and underground coal gasification in Alberta

International Nuclear Information System (INIS)

Haluszka, A.; MacMillan, G.; Maev, S.

2010-01-01

Underground coal gasification has potential in Alberta. This presentation provided background information on underground coal gasification and discussed groundwater and the Laurus Energy demonstration project. A multi-disciplined approach to project assessment was described with particular reference to geologic and hydrogeologic setting; geologic mapping; and a hydrogeologic numerical model. Underground coal gasification involves the conversion of coal into synthesis gas or syngas. It can be applied to mined coal at the surface or applied to non-mined coal seams using injection and production wells. Underground coal gasification can effect groundwater as the rate of water influx into the coal seams influences the quality and composition of the syngas. Byproducts created include heat as well as water with dissolved concentrations of ammonia, phenols, salts, polyaromatic hydrocarbons, and liquid organic products from the pyrolysis of coal. A process overview of underground coal gasification was also illustrated. It was concluded that underground coal gasification has the potential in Alberta and risks to groundwater could be minimized by a properly designed project. refs., figs.

Acetylcholinesterase enzyme activity in carp brain and muscle after acute exposure to diafuran Atividade da enzima acetilcolinesterase em cérebro e músculo de carpas após exposição aguda ao diafuran

Directory of Open Access Journals (Sweden)

Jaqueline Ineu Golombieski

2008-01-01

Full Text Available Sublethal adverse effects may result from exposure of aquatic organisms to insecticides at environmentally relevant concentrations. Fingerlings of the common carp (Cyprinus carpio, Linnaeus, 1758, grass carp (Ctenopharyngodon idella, Valenciennes, 1844, and bighead carp (Aristichthys nobilis, Richardson, 1845 were exposed to diafuran, an insecticide widely used during rice cultivation in Southern Brazil. The aim of this study was to verify the relationship between the lethal concentration (LC50 of diafuran and the acetylcholinesterase (AChE activity in brain and muscle tissues of these species as a possible early biomarker of exposure to this insecticide. LC50 was determined for fish exposed to diafuran concentrations during 96 h (short term: common carp: control, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg L-1; grass carp: control, 1.0, 2.0, 3.0 and 3.5 mg L-1 and, bighead carp: control, 0.5, 1.0, 1.5, 2.0, 3.0 and 4.0 mg L-1, as well as the determination of AChE at concentrations near LC50 for these species. LC50 values (nominal concentrations were 1.81 mg L-1 for the common carp, 2.71 mg L-1 for the grass carp and, 2.37 mg L-1 for the bighead carp. All carps exposed to diafuran were lethargic (lower concentrations or immobile. Diafuran inhibited the acetylcholinesterase activity in brain (~38% and muscle (~50% of all species. Muscle of bighead carp under control treatment showed higher specific AChE activity than brain (14.44 against 5.94 µmol min-1 g protein-1, respectively. Concentrations of diafuran used for rice cropping may affect Cyprinus carpio, Ctenopharyngodon idella and Aristichthys nobilis behaviors and the AChE activities in brain and muscle of these species may be an early biomarker of toxicity of this insecticide.Exposição a inseticidas em concentrações elevadas no ambiente podem ocasionar efeitos adversos subletais em organismos aquáticos. Alevinos de carpa húngara (Cyprinus carpio, Linnaeus, 1758, carpa capim (Ctenopharyngodon

Efficacy of eastern equine encephalitis immunization in whooping cranes.

Science.gov (United States)

Olsen, G H; Turell, M J; Pagac, B B

1997-04-01

An epizootic of eastern equine encephalitis (EEE) at the Patuxent Wildlife Research Center (PWRC), Laurel, Maryland (USA), in 1989 provided an opportunity to determine if EEE immunization protected whooping cranes (Grus americana). Based on seroconversion of 31% of sympatric hatch-year sandhill cranes, Grus canadensis, and a previous 35% case fatality rate in whooping cranes, 17 (37%) of the 46 susceptible whooping cranes should have been exposed to virus and six should have died. As there were no deaths in these birds, the EEE vaccination program appeared to be efficacious in this whooping crane population.

The 1986 ARI (Army Research Institute) Survey of U.S. Army Recruits: Media Habits

Science.gov (United States)

1988-04-01

EDGAR M. JOHNSONCOI Technical Director Cmadn Technical review by Accesion For Curtis L. Gilroy NTAS CRA&:AI Laurel N. Oliver DI A UnC ABm~jc NOTICES...NBC SOURCE T681 X240 SOLID GOLD X241 SOUL TRAIN X242 AMERICAN BANDSTAND X243 DANCE FEVER U" X244 MOVIES ON NETWORK TV X755 MUSIC VIDEOS (NOT...BLACKE’S MAGIC Y080 MOONLIGHTING Y081 MACGIVER Y082 STINGRAY Y083 AIRWOLF Y084 THE A-TEAM Y085 HUNTER Y086 MIAMI VICE Y087 HARDCASTLE AND MCCORMICK Y088

Proceedings of the 6th Nordic Conference on Construction Economics and Organisation

DEFF Research Database (Denmark)

.: DISSATISFACTION FACTORS IN THE INFRASTRUCTURE PROJECTS – PROJECTS FEEDBACK APPROACH Lindahl, G., Blakstad, S., Hansen, G. & Nenonen, S.: USEFRAME – A FRAMEWORK TO UNDERSTAND AND MAP USABILITY RESEARCH Manowong, E.: INFLUENCES OF CONSUMERS-CONSTRUCTORS RELATIONSHIPS IN THE GREEN-BUILDING MARKET Rasila, H., Airo, K...... IN THE BUILT ENVIRONMENT Johansson, T. & Laurell-Stenlund, K.: TIME-GEOGRAPHIC VISUALISATION OF STAKEHOLDER VALUES: A CASE STUDY OF CITY RELOCATION Kjølle, K. & Blakstad, S.: INVOLVING END-USERS’ EXPERIENCE AND AWARENESS: USING BOUNDARY OBJECTS IN BRIEFING Kärnä, S., Manninen, A., Junnonen, J. & Nenonen, S...

El Alcanfor

Directory of Open Access Journals (Sweden)

García Paredes Antonio

1941-08-01

Full Text Available El alcanfor es un cuerpo natural, pertenece al reino vegetal; es blanco, brillante, semitrasparente, aromático, duro, sólido, liviano, inflamable, volátil, pulverizable al ponerle unas gotas de alcohol, soluble en el alcohol y en los aceites, insoluble en el agua, medicinal e industrial. Entre los muchos vegetales que suministra alcanfor el más importante y antiguamente conocido es el Laurel Alcanfor, árbol centenario y frondoso de la familia de las Laurineas, propio del Japón y de la China.

Ploidia e fertilidade de pólen em progênies de citros Ploidy and pollen fertility in citrus hybrids

Directory of Open Access Journals (Sweden)

Eduardo Cesar Brugnara

2008-01-01

Full Text Available Este trabalho foi desenvolvido para estimar a fertilidade do pólen e determinar o nível de ploidia em progênies dos cruzamentos da tangerineira 'Montenegrina' (C. deliciosa Ten. com a tangerineira 'King' (C. nobilis Lour. e com a laranjeira 'Caipira' (C. sinensis (L. Osb.. As plantas, de pés-francos e com idade entre 11 e 12 anos, são mantidas na Estação Experimental Agronômica da Universidade Federal do Rio Grande do Sul, em Eldorado do Sul. Avaliaram-se o nível de ploidia, através da contagem dos cromossomos em células em meiose, e a fertilidade do pólen, por coloração do pólen com carmim propiônico, em 2005 e 2006. Todos os híbridos avaliados são diplóides e a fertilidade de pólen variou de zero a 98%. C27 - híbrido de 'Montenegrina' x 'Caipira' - revelou-se praticamente estéril, e a fertilidade de C21, do mesmo cruzamento, é de 10%. A menor fertilidade observada no cruzamento da 'Montenegrina' x 'King' foi de 42%.This work was performed to estimate pollen fertility and determinate the ploidy level of 11 to 12 years old progenies of crosses of 'Montenegrina' mandarin (Citrus deliciosa Ten. with 'King' mandarin (C. nobilis Lour. and with 'Caipira' sweet orange (C. sinensis (L. Osb. maintained at Estação Experimental Agronômica of UFRGS, in Eldorado do Sul, Brazil. The ploidy level was determined by chromosome countings in meiosis cells and pollen fertility evaluated by staining pollen grains with propionic carmine. All evaluated hybrids are diploid and pollen fertility varied from zero to 98%. C27, a hybrid 'Montenegrina' x 'Caipira', was found is sterile, and C21, from the same cross, showed 10 % fertility. The lowest observed fertility in the progeny 'Montenegrina' x 'King' was 42 %.

Comparison of electron-irradiation and gamma-irradiation as a decontamination treatment of spices

International Nuclear Information System (INIS)

Hayashi, Toru; Todoriki, Setsuko; Mamun.

1993-01-01

Electron-irradiation at 10 kGy decontaminated all the eight kinds of spices (black pepper, white pepper, nutmeg, red pepper, parsley, paprika, laurel and onion powder) to the microbial levels lower than 10 2 /g. Similar sterilization effects by the irradiation were observed on heat-resistant bacterial spores. The 10 kGy irradiation did not significantly affect the contents of essential oils and colors of the spices. These results indicate that electron-irradiation at 10 kGy effectively decontaminates spices without notable adverse effect on the qualities. The disinfecting effect of electron beams on spices was smaller than that of gamma-rays. (author)

The Psychology of Music, editado por Diana Deutsch, 3ª edição: resenha dos capítulos 10 a 13

Directory of Open Access Journals (Sweden)

Ricieri Carlini Zorzal

2016-03-01

Full Text Available O presente texto traz uma resenha de quatro capítulos da terceira edição do livro The Psychology of Music, editado por Diana Deutsch. Nominalmente, são analisados os seguintes capítulos: 10 Performance musical: movimento e coordenação, de autoria de Caroline Palmer; 11 Desenvolvimento musical, de autoria de Laurel Trainor e Erin Hannon; 12 Música e habilidades cognitivas, de autoria de Glenn Schellenberg e Michael Weiss; e 13 As fundações biológicas da música: insights da amusia congênita, de autoria de Isabelle Peretz.

Biodiversity in a Florida Sandhill Ecosystem

Directory of Open Access Journals (Sweden)

Samantha Robertson

2009-01-01

Full Text Available This project compares two transects of land in the University of South Florida's Botanical Gardens for their biodiversity. The transects were chosen to represent a Florida sandhill ecosystem and the individual Longleaf Pine, Saw Palmetto, Turkey Oak, Laurel Oak and Live Oak specimens were counted. All other species above waist height were counted as "other"?. Once the individuals were counted, the Simpson's and Shannon-Wiener indices were calculated. Since the Shannon-Wiener index incorporates several diversity characteristics, it is typically more reliable than Simpson's. However, both biodiversity indices agreed that transect B was more diverse than transect A.

Weathering of dolomite in industrial environments

International Nuclear Information System (INIS)

Gauri, K.L.; Tambe, S.S.; Caner-Saltik, E.N.

1992-01-01

To the exclusion of other major atmospheric pollutants, sulfur dioxide is mainly responsible for attack upon dolomite. This article characterizes Laurel Dolomite on the basis of composition, texture, and porosity; describes the mechanism of the SO 2 reaction with dolomite; and develops two equations for the prediction of the rate of decay of dolomite. Over a period of 120 yr nearly 3.57 mm surface reduction at protected areas and 0.915 mm surface reduction at unprotected surfaces of a building in Louisville was calculated. However, these values could not be verified because measurement of these quantities in the field cannot be made

Eastern equine encephalomyelitis virus and Culiseta melanura activity at the Patuxent Wildlife Research Center, 1985-90.

Science.gov (United States)

Pagac, B B; Turell, M J; Olsen, G H

1992-09-01

Mosquito population densities, virus isolations and seroconversion in sentinel quail were used to monitor eastern equine encephalomyelitis virus (EEE) activity at the Patuxent Wildlife Research Center, Laurel, Maryland, from 1985 through 1990. A dramatic increase in the number of Culiseta melanura collected in 1989, as compared with the 3 previous years, was associated with virus isolations from this species (5/75 pools; n = 542 mosquitoes) and with seroconversion in sentinel quail (4/22 birds positive). This was the first detection of EEE virus activity in this area since a 1984 EEE outbreak killed 7 whooping cranes.

Irradiation of spices produced in Argentina

International Nuclear Information System (INIS)

Kairiyama, E.; Narvaiz, P.; Lescano, G.; Kaupert, N.L.

1988-01-01

Some spices produced in Argentina ground red pepper, anise, fennel, laurel, paprika, Cayenne pepper, cumin (seed), and mayoram (leaves and flowers), were irradiated to inactivate microbial lead, without causing significant chemical or sensory alterations. They were packed in polyethylene bags of 100 m thickness, and irradiated at the 60 Co semi-industrial facility of the Ezeiza Atomic Center, with doses of 7 and 10 kGy, and dose rate of 93.87 Gy/min. Dosimetric data were evaluated with potassium nitrate. Control and irradiated samples were stored at room temperature. Chemical analysis were performed to verify the Argentine Alimentary Codex specifications: water content; essence; total ash; ash insoluble in acid; alcoholic, volatile ether and non-volatile ether extracts; starch; and crude fiber. Besides, colour and volatile substances were analyzed. No differences were found between control and irradiated samples, with the exception of an increase in the total amount of volatiles released by irradiated spices, and a slight colour loss in mayoram. Microbiological determinations consisted of aerobic plate count, yeasts and moulds, coliform bacteria, Salmonella, Staphylococcus aureus, mesophilic and thermophilic sporeformers, enterococci and Clostridium perfringens. Microbial load in control samples oscillated between 10 3 and 10 6 microorganisms per gram, and was inhibited with 10 kGy to undetectable level. With 7 kGy, survivors were still detected in: cumin, anise, laurel, red pepper, fennel and paprika. So, the purpose of this work was accomplished with the dose of 10 kGy. Great improvement on the quality of those products which being stored at room temperature are consumed in the raw state, would be attained. (Author) [es

40 CFR 52.1392 - Federal Implementation Plan for the Billings/Laurel Area.

Science.gov (United States)

2010-07-01

... comprehensive electronic-magnetic report and a written hard copy data summary report. (ii) The electronic report shall be on magnetic or optical media, and such submittal shall follow the reporting format of electronic data being submitted to the MDEQ. EPA may modify the reporting format delineated in this section...

On the Performance of Quorum Replication on the Internet

Science.gov (United States)

2008-10-31

ISP in Cambridge, MA MA2 ISP in Cambridge, MA MA3 ISP in Martha’s Vineyard, MA MA4 ISP in Massachusetts, MA MD ISP in Laurel, MD MEX National...1523 171 96% 58% MA2 28% 742 1517 230 94% 59% NC 32% 465 616 255 90% 63% ISR2 3% 424 70 400 100% 97% UT1 27% 979 1847 189 96% 55% MA1 29% 645 712 238... MA2 UT2 UT1 CA1 AUS NC NZ TW KR CA2 CA3 Fig. 4. Our crumbling wall quorum system. of the first row are in North America. TW, which has lossy

The rate of 45Ca uptake by two corals species at waters of Burung island, Bangka-Belitung province

International Nuclear Information System (INIS)

Zulkifli Dahlan; Gusti Diansyah; T Zia Ulqodry; Ania Citraresmini

2010-01-01

Coral reefs transplantation is the most technique used for coral reefs rehabilitation, at the present. Recently the 45 Ca technique has been using for determining growth appearances in corals because of its ability to calculate the calcification process. For this reason, the study on the rate of 45 Ca uptake by natural corals Acropora Formosa and Acropora nobilis was carried out between June and December 2009 at the waters of Burung Island, Bangka-Belitung Province. The coral fragments of about 5 cm were harvested and put into a PVC container filled with 2 liters of fresh sea water, then incubated with 45 CaCl 2 solutions with an activity of 11.04 μCi/ml for 8 hour under fluorescent light. After the incubation, the “labeled” coral fragments were transplanted to where they have been taken from, and after such period will be re-harvested to determine their 45 Ca uptake content. The results showed that the 45 Ca technique was a reliable method to calculate the rate 45 Ca uptake by coral fragments, which were studied in different depths and time periods of light exposure. There was a significant difference in the 45 Ca uptake by the two different coral species. A. Formosa up took more 45 Ca than A. nobilis did. The highest 45 Ca uptake was shown by A. Formosa at 5 m. This was true for all the lengths of time to light exposure (1, 3, 5 and 7 hours). Different pattern of 45 Ca uptake showed by A. nobilisat 10 m depth, where it could be recognized that after a drop of 45 Ca the uptake increase continuously until the end of the light exposure (7 hours). The difference in 45 Ca uptake between the coral fragments is assumed to be influence by light and the algae species living symbiotically with the coral species that will further influence the CO 2 -fixation. This process will influence the calcification process, which is expressed in 45 Ca uptake. Further studies should be carried out to exactly gathered data of all the factors which could influence the calcification

Discrimination of whisky brands and counterfeit identification by UV-Vis spectroscopy and multivariate data analysis.

Science.gov (United States)

Martins, Angélica Rocha; Talhavini, Márcio; Vieira, Maurício Leite; Zacca, Jorge Jardim; Braga, Jez Willian Batista

2017-08-15

The discrimination of whisky brands and counterfeit identification were performed by UV-Vis spectroscopy combined with partial least squares for discriminant analysis (PLS-DA). In the proposed method all spectra were obtained with no sample preparation. The discrimination models were built with the employment of seven whisky brands: Red Label, Black Label, White Horse, Chivas Regal (12years), Ballantine's Finest, Old Parr and Natu Nobilis. The method was validated with an independent test set of authentic samples belonging to the seven selected brands and another eleven brands not included in the training samples. Furthermore, seventy-three counterfeit samples were also used to validate the method. Results showed correct classification rates for genuine and false samples over 98.6% and 93.1%, respectively, indicating that the method can be helpful for the forensic analysis of whisky samples. Copyright © 2017 Elsevier Ltd. All rights reserved.

Reptilian prey of the sonora mud turtle (Kinosternon sonoriense) with comments on saurophagy and ophiophagy in North American Turtles

Science.gov (United States)

Lovich, J.; Drost, C.; Monatesti, A.J.; Casper, D.; Wood, D.A.; Girard, M.

2010-01-01

We detected evidence of predation by the Sonora mud turtle (Kinosternon sonoriense) on the Arizona alligator lizard (Elgaria kingii nobilis) and the ground snake (Sonora semiannulata) at Montezuma Well, Yavapai County, Arizona. Lizards have not been reported in the diet of K. sonoriense, and saurophagy is rare in turtles of the United States, having been reported previously in only two other species:, the false map turtle (Graptemys pseudogeographica) and the eastern box turtle (Terrapene carolina). While the diet of K. sonoriense includes snakes, ours is the first record of S. semiannulata as food of this turtle. Ophiophagy also is rare in turtles of the United States with records for only five other species of turtles. Given the opportunistic diets of many North American turtles, including K. sonoriense, the scarcity of published records of saurophagy and ophiophagy likely represents a shortage of observations, not rarity of occurrence.

A reply to Iversen et al.'s comment “Monitoring of animal abundance by environmental DNA - An increasingly obscure perspective”

Science.gov (United States)

Klymus, Katy E.; Richter, Catherine A.; Chapman, Duane C.; Paukert, Craig

2015-01-01

We appreciate the conversation put forward by Iversen et al. (2015) in their response to our article “Quantification of eDNA shedding rates from invasive bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix” in the 2015 environmental DNA special issue of Biological Conservation.We agree with Iversen et al.'s concern about overly optimistic conclusions that could be drawn from the current eDNA literature. One hope for eDNA technology is that it can be used in estimating abundance or population density. Evidence suggests that eDNA measurements correlate with total biomass (Takahara et al., 2012) rather than abundance. We demonstrate a similar relationship between biomass and eDNA shedding rates. Nevertheless, without field testing of these methods and specific survey protocols, we cannot make strong conclusions regarding the technique's field applicability. In our manuscript, we attempted to point out areas in which more research is needed.

Identification, characterization, and palynology of high-valued medicinal plants.

Science.gov (United States)

Fazal, Hina; Ahmad, Nisar; Haider Abbasi, Bilal

2013-01-01

High-valued medicinal plants Achillea millefolium, Acorus calamus, Arnebia nobilis, Fumaria indica, Gymnema sylvestre, Origanum vulgare, Paeonia emodi, Peganum harmala, Psoralea corylifolia, Rauwolfia serpentina, and Vetiveria zizanioides were identified with the help of taxonomical markers and investigated for characterization and palynological studies. These parameters are used to analyze their quality, safety, and standardization for their safe use. Botanical description and crude drug description is intended for their quality assurance at the time of collection, commerce stages, manufacturing, and production. For this purpose the detailed morphology was studied and compared with the Flora of Pakistan and other available literatures. Here we reported the pollen grain morphology of Origanum vulgare, Paeonia emodi, Psoralea corylifolia, and Rauwolfia serpentina for the first time. Similarly the crude drug study of Gymnema sylvestre (leaf), Origanum vulgare (aerial parts), Paeonia emodi (tubers), and Peganum harmala (seeds) was also carried out for the first time.

Identification, Characterization, and Palynology of High-Valued Medicinal Plants

Directory of Open Access Journals (Sweden)

Hina Fazal

2013-01-01

Full Text Available High-valued medicinal plants Achillea millefolium, Acorus calamus, Arnebia nobilis, Fumaria indica, Gymnema sylvestre, Origanum vulgare, Paeonia emodi, Peganum harmala, Psoralea corylifolia, Rauwolfia serpentina, and Vetiveria zizanioides were identified with the help of taxonomical markers and investigated for characterization and palynological studies. These parameters are used to analyze their quality, safety, and standardization for their safe use. Botanical description and crude drug description is intended for their quality assurance at the time of collection, commerce stages, manufacturing, and production. For this purpose the detailed morphology was studied and compared with the Flora of Pakistan and other available literatures. Here we reported the pollen grain morphology of Origanum vulgare, Paeonia emodi, Psoralea corylifolia, and Rauwolfia serpentina for the first time. Similarly the crude drug study of Gymnema sylvestre (leaf, Origanum vulgare (aerial parts, Paeonia emodi (tubers, and Peganum harmala (seeds was also carried out for the first time.

Rapid establishment of pure lines of silver carp (Hypophthalmichthys molirix) and bighead carp (Aristichthys nobilis)

Institute of Scientific and Technical Information of China (English)

WANG Zhongwei; YE Yuzhen; ZHOU Jianfeng; WU Qingjiang

2004-01-01

The diversity of gynogenetic, artificial sex reversal and natural silver carp and bighead carp is examined using randomly amplified polymorphic DNA (RAPD) method.All of the 187 bands are obtained and 19 (10.16%) of them are polymorphic in gynogenetic silver carp.Meanwhile 32 (15.61%) out of 205 bands are polymorphic in control group.In gynogenetic bighead carp a total of 232 bands are identified and 11 (4.74%) out of them are polymorphic, while 25 (10.37%) out of 241 bands are polymorphic in control group.The genetic distance of four populations is calculated and it is 0.102 and 0.023 for gynogenetic silver carp and gynogenetic bighead carp respectively.The values of natural silver carp and bighead carp are 0.161 and 0.104.From the UPGMA trees constructed based on genetic distance, the sex reversal individuals that match with the gynogenetic female individuals are picked out.A new breeding process of establishing a pure line is developed.

The resurrection of Charybdis (Gonioinfradens) giardi (Nobili, 1905), newly recorded from the SE Mediterranean Sea.

Science.gov (United States)

Galil, Bella S; Douek, Jacob; Gevili, Roy; Goren, Menachem; Yudkovsky, Yana; Paz, Guy; Rinekvich, Baruch

2018-01-15

A single adult specimen of Gonioinfradens giardi, a portunid crab known from the Red Sea, Gulf of Oman and Arabian Gulf, was recently collected off the southern Israeli coast, in the southeastern Mediterranean Sea. Morphological characters, as well as molecular analyses based on the mitochondrial barcoding gene cytochrome oxidase sub unit I (COI), support its distinction from the widely distributed G. paucidentata. Therefore, G. giardi is reinstated as a valid species, and withdrawn from its synonymy with G. paucidentata. Previous Mediterranean records of the latter species are misidentifications and should be referred to G. giardi. The species is described, illustrated, and differentiated from its cogener.

Plasticizers and BPA Residues in Tunisian and Italian Culinary Herbs and Spices.

Science.gov (United States)

Di Bella, Giuseppa; Ben Mansour, Hedi; Ben Tekaya, Asma; Beltifa, Asma; Potortì, Angela Giorgia; Saiya, Emanuele; Bartolomeo, Giovanni; Dugo, Giacomo; Lo Turco, Vincenzo

2018-06-01

In the present study, 18 plasticizers and residues in 10 different Tunisian and Italian culinary herbs and spices (black pepper, mint, caraway, coriander, oregano, rosemary, thyme, fennel, verbena, and laurel) were determined by GC/MS. Di-methylphthalate, di-(2-methylpropyl)adipate, di-n-butyladipate, di-propylphthalate, benzylbenzoate, di-phenylphthalate, and bisphenol A were lower than their LOQ in all 118 samples. Among the Tunisian samples, di-(2-ethylhexyl)phthalate was found in all types of samples and di-butylphthalate in 50% of types; all other phthalates were rarely dectected. Among the adipates, only di-methyladipate was found in 90% of types; di-ethyladipate was seldom found and di-(2-ethylhexyl)adipate only in samples of caraway. Di-(2-ethylhexyl)terephthalate was found in all types of samples; di-(2-ethylhexyl)sebacate was detected rarely but with high values. Among the Sicilian samples, di-ethylphthalate and di-(2-methylpropyl)phthalate were detected only in samples of mint; di-methyladipate, di-butylphthalate, and benzylbutylphthalate were identified in oregano and laurel; di-(2-ethylhexyl)terephthalate and di-(2-ethylhexyl)sebacate only in oregano. In any case, the results suggested that intake of these contaminants through spices and herbs is not a dangerous risk to the consumers. Probably, as already observed for the other food, these contaminants could result from pollution of the environment (air, water, and/or soil) and/or farming methods. Plasticizers and BPA in Tunisian and Italian spices were determined by GC/MS. Tunisian spices seem to contain more residues than Italian samples. Intake of these contaminants by spices is not a dangerous risk to the consumers. These pollutants could result from environmental pollution or agricultural practices. © 2018 Institute of Food Technologists®.

Ciudades soñadas y ciudades en las que es imposible soñar: la narrativa de Guinea Ecuatorial

Directory of Open Access Journals (Sweden)

Dorothy Odartey-Wellington

2012-11-01

Full Text Available Este trabajo examina las contribuciones de los escritores contemporáneos de Guinea Ecuatorial a la construcción de nociones de ciudades y sus habitantes en la narrativa africana contemporánea. Aunque el documento se basa principalmente en obras de María Nsue Angüe, Maximiliano Nkogo Esono, Juan Tomás Ávila Laurel, Donato Ndongo-Bidyogo y César Mba Abogo, la interpretación de estos escritores de las ciudades se examina en relación con un contexto más amplio de la escritura africana representada por los siguientes autores anglófonos, Ama Ata Aidoo, Sefi Atta, Kojo Laing y Sinyangwe Binwell así como la francófona Calixthe Beyala. El tratamiento particular de ciudades por parte de los escritores guineoecuatorianos, en comparación con las tendencias en otros ejemplos de la literatura y crítica africanas, tiene su origen en las preocupaciones socio-políticas en el contexto de la petroeconomía de su país. This paper examines the contributions of contemporary Equatoguinean writers to the construction of ideas of cities and their inhabitants in contemporary African narrative. Although the paper draws mainly on works by María Nsue Angüe, Maximiliano Nkogo Esono, Juan Tomás Ávila Laurel, Donato Ndongo-Bidyogo and César Mba Abogo, these writers’ rendition of cities is examined with reference to a wider context of African writing represented by Anglophone and Francophone writers Ama Ata Aidoo, Sefi Atta, Kojo Laing, Binwell Sinyangwe and Calixthe Beyala. The particular treatment of Equatoguinean cities in comparison to trends in other examples of African literature and criticism, are traced to the socio-political concerns in the context of Equatorial Guinean new petro-economy.

PRODUCCIÓN DE MADERA Y CARBONO EN LA REGENERACIÓN DE SISTEMAS AGROFORESTALES EN LA RESERVA INDÍGENA DE TALAMANCA, COSTA RICA

Directory of Open Access Journals (Sweden)

Wilson A. Calero Borge

2012-02-01

Full Text Available El trabajo se desarrolló en la Reserva Indígena de Talamanca, Costa Rica. Se remidieron 64 parcelas de cacao (C y banano (B, para estimar el almacenamiento y el incremento de madera y carbono de laurel (Cordia alliodora y cedro amargo (Cedrela odorata L. de regeneración natural en estos sistemas agroforestales (SAF. Se realizó inventario de laurel y cedro real y se midieron árboles con d3 4 cm. El volumen comercial (Vc (d 3 45 cm se proyectó con matrices de transición tipo Usher.Los incrementos de madera y carbono se obtuvieron en el períodocomprendido 2001-2005.No hubo diferencias signifi cativas (P<0.05 para la densidad (d y existencias totales de madera (Vt en los SAF, la d es de 52,4 árboles ha-1 en C y 53,8 árboles ha-1 en B, contienen un volumen total de 55,4 m3 ha-1 en C y 52,6 m3 ha-1 en B. Las tasas de fi jación de carbono son de 1,3 y 1,8 t ha-1 año-1 en C y B respectivamente.A cinco años de proyección los C producirán 52,0 (m3 ha-1 y los B 54,2 (m3 ha-1 de Vc con un IPAVc de 4,08 y 4,74 (m3 ha-1 año-1 en C y B respectivamente. Para todos los SAF evaluadosel aprovechamiento de madera será sostenible, con las actuales tasas de aprovechamiento y reclutamiento de individuos. Se recomienda aplicar del modelo propuesto para el diseño ymanejo de SAF.

A comparative analysis of terrestrial arthropod assemblages from a relict forest unveils historical extinctions and colonization differences between two oceanic islands

Science.gov (United States)

Matthews, Thomas J.; Rego, Carla; Crespo, Luis; Aguiar, Carlos A. S.; Cardoso, Pedro; Rigal, François; Silva, Isamberto; Pereira, Fernando; Borges, Paulo A. V.; Serrano, Artur R. M.

2018-01-01

During the last few centuries oceanic island biodiversity has been drastically modified by human-mediated activities. These changes have led to the increased homogenization of island biota and to a high number of extinctions lending support to the recognition of oceanic islands as major threatspots worldwide. Here, we investigate the impact of habitat changes on the spider and ground beetle assemblages of the native forests of Madeira (Madeira archipelago) and Terceira (Azores archipelago) and evaluate its effects on the relative contribution of rare endemics and introduced species to island biodiversity patterns. We found that the native laurel forest of Madeira supported higher species richness of spiders and ground beetles compared with Terceira, including a much larger proportion of indigenous species, particularly endemics. In Terceira, introduced species are well-represented in both terrestrial arthropod taxa and seem to thrive in native forests as shown by the analysis of species abundance distributions (SAD) and occupancy frequency distributions (OFD). Low abundance range-restricted species in Terceira are mostly introduced species dispersing from neighbouring man-made habitats while in Madeira a large number of true rare endemic species can still be found in the native laurel forest. Further, our comparative analysis shows striking differences in species richness and composition that are due to the geographical and geological particularities of the two islands, but also seem to reflect the differences in the severity of human-mediated impacts between them. The high proportion of introduced species, the virtual absence of rare native species and the finding that the SADs and OFDs of introduced species match the pattern of native species in Terceira suggest the role of man as an important driver of species diversity in oceanic islands and add evidence for an extensive and severe human-induced species loss in the native forests of Terceira. PMID:29694360

Parentesco reconfigurado no espaço da adoção Refiguring kinship in the space of adoption

Directory of Open Access Journals (Sweden)

Barbara Yngvesson

2007-12-01

Full Text Available Nesse artigo, inspiro-me em pesquisas realizadas na Suécia, Índia, Colômbia, Equador, Bolívia, Chile e Estados Unidos, entre 1995 e 2004, para enfocar o que Laurel Kendall descreve como "a plasticidade assim como a força do parentesco idiomático" no contexto da adoção transnacional. Tanto na Europa como na América do Norte, adoções transnacionais (que tendem a ser transraciais, dado que a maioria das crianças adotadas vem de regiões não-européias complicam o projeto de "imitar a natureza", pois em muitas dessas adoções a "diferença" entre os pais adotivos e a criança é evidente. O adotado oriundo da Ásia, da África ou da América Latina que vive numa família euro-americana representa um paradoxo de pertencimento no contexto global onde o transnacionalismo, ao mesmo tempo, afirma e transgride as fronteiras da nação-estado.In this article, I draw on research carried out in Sweden, India, Colombia, Ecuador, Bolivia, Chile, and the United States between 1995-2004 to focus on what Laurel Kendall describes as "the plasticity as well as the power of idiomatic kinship" in the context of transnational adoption. In both Europe and North America, transnational adoptions (which have tended to be cross-racial, in the sense that the majority of adopted children come from non-European regions of the globe complicate the project of "imitating nature", since in many of these adoptions, the "difference" between adopted child and parent is obvious. The adoptee from Asia, Africa, or Latin America in an Euro-American home represents the ultimate paradox of belonging in a global context where transnationalism both affirms and breaches the borders of the nation-state.

A critical reflection on the technological development of deep brain stimulation (DBS

Directory of Open Access Journals (Sweden)

Christian eIneichen

2014-09-01

Full Text Available Since the translational research findings of Benabid and colleagues, which partly led to their seminal paper regarding the treatment of mainly tremor-dominant Parkinson patients through thalamic high-frequency-stimulation (HFS in 1987, we still struggle with identifying a satisfactory mechanistic explanation of the underlying principles of Deep Brain Stimulation. Furthermore, the technological advance of DBS devices (electrodes and implantable pulse generators, IPG's has shown a distinct lack of dynamic progression. In light of this we argue that it is time to leave the paleolithic age and enter hellenistic times: the device-manufacturing industry and the medical community together should put more emphasis on advancing the technology rather than resting on their laurels.

Understanding Sediment Processes of Los Laureles Canyon in the Binational Tijuana River watershed

Science.gov (United States)

Tijuana River Basin originates in Mexico and drains 4465 km2 into the Tijuana River Estuary National Research Reserve, a protected coastal wetland in California that supports 400 species of birds. During storms, excessive erosion in Tijuana produces sediment loads that bury nativ...

Biological control of tanoak and bay laurel resprouts using the fungus, Chondrostereum purpureum

Science.gov (United States)

Marianne Elliott; Gary Chastagner; Simon Shamoun; Grace Sumampong; Ellen Goheen; Alan Kanaskie

2013-01-01

In southwest Oregon, an aggressive program of cutting and burning host plants in an effort toeradicate Phytophthora ramorum was initiated. It was soon apparent that tanoak (Notholithocarpus densiflorus (Hook.& Arn.) Manos, Cannon& S.H. Oh) resprouts were highly susceptible to P. ramorum and...

Replacing fish meal by food waste in feed pellets to culture lower trophic level fish containing acceptable levels of organochlorine pesticides: health risk assessments.

Science.gov (United States)

Cheng, Zhang; Mo, Wing-Yin; Man, Yu-Bon; Nie, Xiang-Ping; Li, Kai-Bing; Wong, Ming-Hung

2014-12-01

The present study used food waste (collected from local hotels and restaurants) feed pellets in polyculture of low-trophic level fish [bighead (Aristichtys nobilis), grass carp (Ctenopharyngodon idellus), and mud carp (Cirrhina molitorella)] aiming at producing safe and quality products for local consumption. The results indicated that grass carp (hexachlorocyclohexanes (HCHs) waste feed pellets were relatively free of organochlorine pesticides (OCPs). The experimental ponds (water and sediment) were relatively free of OCPs, lowering the possibility of biomagnification of OCPs in the food chains within the ponds. The raw concentrations of OCPs extracted from the fish were not in the bioavailable form, which would ultimately reach bloodstream and exert adverse effects on human body. Health risk assessments based on digestible concentrations are commonly regarded as a more accurate method. The results of health risk assessments based on raw and digestible concentrations showed that the fish fed with food waste feed pellets were safe for consumption from the OCP perspective. Copyright © 2014 Elsevier Ltd. All rights reserved.

Posthodiplostomatosis in a fishpond in Serbia

Directory of Open Access Journals (Sweden)

Marković Maja

2012-01-01

Full Text Available Posthodiplostomatosis (black spot disease is a disease of young freshwater fish species of families Cyprinidae and Cobitidae, resulting from infection with metacercaria, which is the penultimate developmental stage of Posthodiplostomum cuticola. Metacercaria give rise to black cysts located primarily in the skin, muscles and on the fins. The disease caused by P. cuticola in certain fish species is first detected in a fishpond in Serbia, as presented in the current work, whilst in natural waters it has been existing for many years. Diagnosis of the infection in the grass carp (Ctenopharyngodon idella and the bighead carp (Aristichthys nobilis was based on the occurrence of dark discolorations and cysts of the parasite on the fins. Cysts measured approximately 1 mm in diameter, were confirmed by histopathological means. Posthodiplostomatosis occurred in fishponds in which preventive measures were not implemented, i.e. where the nurseries were not dried up and mechanically cleaned.

HL-60 differentiating activity and flavonoid content of the readily extractable fraction prepared from citrus juices.

Science.gov (United States)

Kawaii, S; Tomono, Y; Katase, E; Ogawa, K; Yano, M

1999-01-01

Citrus plants are rich sources of various bioactive flavonoids. To eliminate masking effects caused by hesperidin, naringin, and neoeriocitrin, the abundant flavonoid glycosides which make up 90% of the conventionally prepared sample, the readily extractable fraction from Citrus juice was prepared by adsorbing on HP-20 resin and eluting with EtOH and acetone from the resin and was subjected to HL-60 differentiation assay and quantitative analysis of major flavonoids. Screening of 34 Citrus juices indicated that King (C. nobilis) had a potent activity for inducing differentiation of HL-60, and the active principles were isolated and identified as four polymethoxylated flavonoids, namely, nobiletin, 3,3',4',5,6,7, 8-heptamethoxyflavone, natsudaidain, and tangeretin. HPLC analysis of the readily extractable fraction also indicated that King contained high amounts of these polymethoxylated flavonoids among the Citrus juices examined. Principal component and cluster analyses of the readily extractable flavonoids indicated peculiarities of King and Bergamot.

Distillation of Essential Oils from Pontianak Orange Peel Wastes and Its Utilization for Aromatherapy Soap

Directory of Open Access Journals (Sweden)

Hidayati Hidayati

2012-12-01

Full Text Available Orange (Citrus nobilis var. microcarpa is the main commodities in Pontianak. Production of essential oils from orange peel wastes and its use for soap aromatherapy substance could improve its economic value. This study is aimed to produce the essential oils from orange peel wastes with the highest limonene content by distillation. Its application for aromatherapy soap substance also evaluated. Distillation of essential oils from orange peels was performed at 1000C and 1100C for 4, 5, 6 and 7 hours. The results showed that at 1000C for 7 hours produced the highest limonene content, reach 97.69%. The essential oils color was pale yellow, specific gravity 0.84, refractive index 1.47, solubility in 90% ethanol 1:1 (transparent, acid value 0.143% and ester number 5.37. The aromatherapy soap produced with addition of 3.6% of limonene oils is in accordance with SNI 06-3532-1994 except for water content parameter.

Radioactive contamination of the significant fish species in fish-ponds and in natural waters with particular respect to the accumulation of Sr-90 Pt. 2

International Nuclear Information System (INIS)

Kantor, D.; Szentjobi, O.

1977-01-01

The radioactive contamination of omnivorous fish species (Cyprinus carpio, Hypophtalmictys nobilis Richardson), of herbivorous fish species (Hypophtalmictys molitrix Valenciennes) and carnivorous fish species (Silurus glanis L., Esox Lucius L.) has been investigated. Orientative data have been obtained by analyzing samples originating from various sites. Though differences appeared between the analyzed samples, no incorporations of outstanding nature have been observed. In accordance with experiences described in literature, the isotope Sr-90 is accumulated in an increased degree in the skeleton of fish living in ponds. This is valid in all cases when the vital processes are regulated artificially by fishery methods. A particularly important factor is that the individual fish living in ponds are 2-3 or at most 4 years of age i.e. from the aspect of their ontogenesis their organism is in a strongly build-up phase. Sr-90 proved to be accumulated in a relatively greater amount in the organisms of herbivorous fish. (P.J.)

The Types of Essentials Oil Components Isolated From the Leaves of Citrus Aurantifolia and Citrus Nobilis

OpenAIRE

Wulandari, Mutiara Juni; Mohammad Anwar Jamaludin,, Lailatul Riska, Agustin Laela Prunama; Mumun Nurmilawati, Indra Fauzi

2015-01-01

Essential oil or known as the eteris oil (etheric oil) was result from secondary metabolism of a plant. In general essential oil contains of citronellal, Citronelal, Citronelol, Limonen, β-Pinene dan sabinene. The components essential oil derived from citrus plants commonly used by perfume industry, on other hand it is used as essentials oil orange flavour addition in some drinks and food, and also as an antioxidant and anti cancer. One of the essential oil is produced by Citrus aurantifolia ...

Anti-mycobacterial activity of polyketides from Penicillium sp. endophyte isolated from Garcinia nobilis against Mycobacterium smegmatis

OpenAIRE

Jean Bosco Jouda; Isabelle Kamga Mawabo; Augustin Notedji; Céline Djama Mbazoa; Jean Nkenfou; Jean Wandji; Céline Nguefeu Nkenfou

2016-01-01

Objective/background: According to estimates by the World Health Organization, there were 9.6 million new tuberculosis (TB) cases in 2014: 5.4 million among men, 3.2 million among women, and 1.0 million among children. There were also 1.5 million TB deaths. Although there are potent anti-TB molecules, the misuse of these drugs in addition to inconsistent or partial treatment have led to the development of multidrug-resistant TB and extensively drug-resistant TB. It is established that plants ...

DOE ZERH Case Study: Habitat for Humanity South Sarasota, Laurel Gardens #794, Nakomis, FL

Energy Technology Data Exchange (ETDEWEB)

none,

2015-09-01

Case study of a DOE 2015 Housing Innovation Award winning affordable home in the hot-humid climate that got a HERS 51 without PV, with foam-filled masonry block walls with .75” rigid foam, furring strips, and foil-faced paper on interior walls; R-20 ocsf in roof of sealed attic, uninsulated slab, 15 SEER 8.0 HSPF heat pump walls for heating and cooling, heat pump water heater.

Unlocking Sydney's renewable gas potential

International Nuclear Information System (INIS)

Ong, Jacqueline

2014-01-01

The City of Sydney may have hit the statewide resource recovery target of 66% two years early, but it is not resting on its laurels. Instead, it wants to do more with the remaining 34% of non-recyclable waste that is being sent to landfill. Its Advanced Waste Treatment Master Plan explores how the waste-derived gas can be injected itito the gas grid to fuel local energy generation or transport networks, killing two birds with one stone - meeting the city's energy needs and diverting waste from landfill. The plan seems sound. After all, landfill costs have increased 300% in the past decade and landfill levies are projected to exceed $400 a tonne by 2030. In addition, four landfills sites operating near the city will close within the next few years

RIA of alpha-fetoprotein in serum and amniotic fluid

Energy Technology Data Exchange (ETDEWEB)

Fingerova, H; Talas, M; Stroufova, A [Palackeho Univ., Olomouc (Czechoslovakia). Lekarska Fakulta; Santavy, J; Krikal, Z [Ustav pro Peci o Matku a Dite, Prague (Czechoslovakia)

1979-01-01

An own modification of the double antibody radioimmunoassay for AFP using /sup 125/I-labelled AFP as a tracer, rabbit anti-AFP obtained from SEVAC, Prague and precipitating antibodies prepared by the authors is described. The AFP levels measured in the serum and the amniotic fluid using the method were in agreement with those obtained by the means of the AFPK RIA kit by SORIN in the Institute for the Care of Mother and Child in Prague. The AFP concentrations found in the cord serum and the amniotic fluid were confirmed also by the rocket electroimmunoassay according to Laurell. The described AFP RIA seems suitable for the clinical application in prenatal screening for congenital malformations, in difficult pregnancies, in hepatology and the diagnosis and the evaluation of therapy of some human malignancies.

Occupational health I: The method of collective interviews applied to the study of the work-weariness process of workers of alcohol distilleries in the region of Ribeirao Preto; Saude do trabalhador I: o metodo de entrevistas coletivas aplicado ao estudo do processo trabalho-desgaste operario em destilaria de alcool na regiao de Ribeirao Preto

Energy Technology Data Exchange (ETDEWEB)

Azevedo Pinheiro, Sandra de; Ruffino Netto, Antonio [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Medicina. Dept. de Medicina Social

1994-07-01

The author presents the method of investigation used in the study of the work-weariness process of workers of alcohol distilleries in the region of Ribeirao Preto - Sao Paulo State, Southeast Brazil. The study was based on the methodology proposed by Laurell and Noriega (1989), and on the collective interviews carried out with groups of workers from four large sectors of an alcohol distillery: the patio, the crushing sector, the boilers and the evaporation sector, and the fermentation and distillation sectors. The information obtained from the workers and collectively agreed by them, was compares to other sources of data: notification of the occupational accidents, reports of the Regional Labor Office, direct observation of the industrial environment, items of the collective agreement, and supplementary clinic-laboratory tests, among others. (author) 24 refs.

RIA of alpha-fetoprotein in serum and amniotic fluid

International Nuclear Information System (INIS)

Fingerova, H.; Talas, M.; Stroufova, A.

1979-01-01

An own modification of the double antibody radioimmunoassay for AFP using 125 I-labelled AFP as a tracer, rabbit anti-AFP obtained from SEVAC, Prague and precipitating antibodies prepared by the authors is described. The AFP levels measured in the serum and the amniotic fluid using the method were in agreement with those obtained by the means of the AFPK RIA kit by SORIN in the Institute for the Care of Mother and Child in Prague. The AFP concentrations found in the cord serum and the amniotic fluid were confirmed also by the rocket electroimmunoassay according to Laurell. The described AFP RIA seems suitable for the clinical application in prenatal screening for congenital malformations, in difficult pregnancies, in hepatology and the diagnosis and the evaluation of therapy of some human malignancies. (author)

As condições de trabalho como fator desencadeador do uso de substâncias psicoativas pelos trabalhadores de enfermagem Las condiciones de trabajo como factor desencadenante del uso de sustancias psicoactivas por los trabajadores de enfermería The work conditions as unleash factor of the psychoactive substance use by the workers of nursing

Directory of Open Access Journals (Sweden)

Elizabeth Rose Costa Martins

2007-12-01

Full Text Available O estudo teve como objeto o uso de substâncias psicoativas pelos trabalhadores de enfermagem e sua relação com o trabalho. O objetivo foi analisar as condições de trabalho como fator desencadeador do uso de drogas pelo trabalhador de enfermagem. O referencial teórico estruturou-se a partir do conceito de drogas e modelos explicativos da drogodependência, apoiados nas concepções de Dejours (1999 e Laurell & Noriega (1989. Estudo descritivo de abordagem qualitativa com 40 sujeitos trabalhadores de enfermagem de clínica médica de um hospital universitário no Município do Rio de Janeiro, realizado em 2005 e 2006. Como instrumento de coleta de dados utilizou-se a entrevista semi-estruturada. Os resultados mostraram que a utilização de substâncias psicoativas pelos profissionais no local de trabalho pode estar relacionada com as condições de sobrecarga de trabalho e com a facilidade de acesso e que tais substâncias comprometem a sua saúde e o desenvolvimento de suas atividades laborais.El estudio tiene como objeto el uso de substancia psicoactivas por los trabajadores de enfermería y su relación con el trabajo. El objetivo fue analizar las condiciones de trabajo como factor desencadenante al uso de drogas por el trabajador de enfermeria. El referencial teórico se estructuró a partir del concepto de drogas y modelos explicativos de la drogodependencia, apoyado en las concepciones de Dejours (1999 y Laurell y Noriega (1989. Estudio descriptivo y enfoque cualitativo con 40 sujetos trabajadores de enfermeria, de clínica médica de un hospital universitário del Municipio de Rio de Janeiro- Brasil, realizado en 2005 y 2006. Como instrumento de recolección de datos, la entrevista semiestructurada. Los resultados revelan que la utilización de substancias psicoactivas, por los profesionales, en el local de trabajo, puede estar relacionada con las condiciones y carga excesiva de trabajo, con la facilidad de acesso y con las

Hydraulic and water-quality data collection for the investigation of Great Lakes tributaries for Asian carp spawning and egg-transport suitability

Science.gov (United States)

Murphy, Elizabeth A.; Jackson, P. Ryan

2013-01-01

If the invasive Asian carps (bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix) migrate to the Great Lakes, in spite of the efforts to stop their advancement, these species will require the fast-flowing water of the Great Lakes tributaries for spawning and recruitment in order to establish a growing population. Two Lake Michigan tributaries (the Milwaukee and St. Joseph Rivers) and two Lake Erie tributaries (the Maumee and Sandusky Rivers) were investigated to determine if these tributaries possess the hydraulic and water-quality characteristics to allow successful spawning of Asian carps. To examine this issue, standard U.S. Geological Survey sampling protocols and instrumentation for discharge and water-quality measurements were used, together with differential global positioning system data for georeferencing. Non-standard data-processing techniques, combined with detailed laboratory analysis of Asian carp egg characteristics, allowed an assessment of the transport capabilities of each of these four tributaries. This assessment is based solely on analysis of observed data and did not utilize the collected data for detailed transport modeling.

Decontamination of Clostridium perfringens and Salmonella spp. in Thai Fermented Fish (Pla-ra) by Gamma Radiation

International Nuclear Information System (INIS)

Prakhongsil, P.; Phianphak, W.; Malakrong, A.; Komolamisra, C.

2014-01-01

Gamma radiation can be applied as a decontamination method to eliminate microorganisms in fermented food. In this study, samples of Thai fermented fish were evaluated for microbiological and hygienic qualities and then exposed to gamma irradiation. Prior to irradiation, Salmonella spp. and Clostridium perfringens were detected and the results were found contaminated in five samples from twenty-six of Thai fermented fish samples ; Nile tilapia fish (Oreochromisniloticus), bighead carp fish (Aristichthys nobilis) and common snakehead fish (Channa striata) using VIDAS Salmonella Easy SLM assay and standard conventional assay for C. perfringens. For detecting of living parasites helminths, fifteen samples were assayed for liver fluke (Opisthorchis viverrini) and Gnathostoma spinigerum, but neither was found. When exposed to gamma irradiation, results showed that the minimum dose of 2.70 kGy could sufficiently eliminate Salmonella spp. from fermented Nile tilapia fish (Oreochromis nioloticus), whereas a higher dose of 6.16 kGy was required to reduce C. perfringens from130 CFU/g and 10 CFU/g to less than 10 CFU/g in fermented Nile tilapia fish and common snakehead fish (Channa striata) fish.

Not all carp are created equal: Impacts of broadband sound on common carp swimming behavior

Science.gov (United States)

Murchy, Kelsie; Vetter, Brooke J.; Brey, Marybeth; Amberg, Jon J.; Gaikowski, Mark; Mensinger, Allen F.

2016-01-01

Bighead carp (Hypophthalmichthys nobilis), silver carp (H. molitrix) (hereafter: bigheaded carps), and common carp (Cyprinus carpio) are invasive fish causing negative impacts throughout their North American range. To control their movements, non-physical barriers are being developed. Broadband sound (0.06 to 10 kHz) has shown potential as an acoustic deterrent for bigheaded carps, but the response of common carp to broadband sound has not been evaluated. Since common carp are ostariophysians, possessing Weberian ossicles similar to bigheaded carps, it is possible that sound can be used as an acoustical deterrent for all three species. Behavioral responses to a broadband sound were evaluated for common carp in an outdoor concrete pond. Common carp responded a median of 3.0 (1st Q: 1.0, 3rd Q: 6.0) consecutive times to the broadband sound which was lower than silver carp and bighead carp to the same stimulus. The current study shows that common carp demonstrate an inconsistent negative phonotaxis response to a broadband sound, and seem to habituate to the sound quickly.

Selected medicinal plants used in herbal industries; their toxicity against pathogenic microorganisms

International Nuclear Information System (INIS)

Fazal, H.; Ahmad, M.; Abbasi, B.H.

2012-01-01

Multi-drug resistant strains of fungi and bacteria are imposing the need for new drugs. Reliable natural sources with minor side effects are needed to control anti-human pathogenic invaders specially bacteria. Given the demands for natural products that are inherently safe and environmentally compatible, the advancement in antimicrobial potential has provided a better alternative to synthetic resistance antibiotics. In the present investigation such types of medicinal plants were selected for analyses that are used by local herbal practioners for multiple diseases. Thirty three extracts of Achillea millefolium, Acorus calamus, Arnebia nobilis, Fumaria indica, Gymnema sylvestre, Origanum vulgare, Paeonia emodi, Peganum harmala, Psoralea corylifolia, Rauwolfia serpentina and Vetiveria zizanioides in chloroform, ethanol and hexane were investigated for their antimicrobial potential. These extracts were tested against eight microorganisms including four gram negative bacterial strains viz., Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhi, three gram positive bacterial strains Staphylococcus aureus, Bacillus subtilis and Bacillus cereus and a fungal strain viz., Candida albicans. Majority of the extracts showed marked antimicrobial potential against the tested microorganisms. (author)

I pionieri italiaNi della geofisiCa

Directory of Open Access Journals (Sweden)

P. CALOI

1964-06-01

Full Text Available Vengono rapidamente passati in rassenga, seguendo
l'ordine cronologico, gli studiosi di geofisica — intesa in senso lato — clie,
a partire dalla seconda meta del Settecento, ebbero ad interessarsi in Italia
di elettricita atmosferica, magnetismo terrestre, idrografla, oceanografia fisica,
sismologia e vulcanologia, con intenti pionieristici. Sono ricordati i principali
risultati raggiunti da una trentina di ricercatori fra i quali spiccano, per la
vastita del lavoro compiuto e per la novita dei risultati, Leopoldo Nobili e Macedonio
Melloni. Ma, ciascuno nei suo campo, scoperte di rilievo fecero pure
G. B. Beccaria, Luigi Palmieri, Carlo Matteucci, Timoteo Bertelli, Luigi De
Marcbi, Carlo Somigliana, Emilio Oddone, Alfonso Sella e Domenico Pacini.
Alcuni vantano priorita di rilievo: scoperta della doppia oscillazione diurna
del barometro (Chiminello, 1780; unicita sorgiva delle varie radiazioni dello
spettro (M. Melloni; sismografo elettromagnetico a registrazione continua
(Palmieri, 1855; microsismi (Bertelli, 1872; il paleomagnetismo (Oddone e
Sella, 1891; la radiazione cosmica (Pacini, 1910; . ..

Environmental DNA calibration study. Interim technical review report

Science.gov (United States)

Baerwaldt, K.; Bartron, Meredith L.; Schilling, K.; Lee, Debbie; Russo, Edmond; Estes, Trudy; Fischer, Richard; Fleming, Beth; Guilfoyle, Michael P.; Kilgore, K. Jack; Lance, Richard; Perkins, Edward; Schultz, Martin; Smith, David; Amberg, Jon J.; Chapman, Duane C.; Gaikowski, Mark P.; Klymus, Katy E.; Richter, Catherine A.

2014-01-01

Invasive aquatic nuisance species pose a major threat to aquatic ecosystems worldwide. Invasive Asian carps, including bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix) have been steadily dispersing upstream through the Mississippi, Illinois, and Des Plaines Rivers since the 1990s. To prevent further movement up the Illinois River into the Chicago Area Waterway System (CAWS, see Figures 1.1.1 to 1.1.3) and possibly Lake Michigan and the Great Lakes ecosystem, an electrical barrier has been operating near Lockport to deter the advance of Asian carp. Although one adult individual has been detected in Lockport pool of the Illinois Waterway, the leading edge of the invasion of bighead and silver carp is considered to be at RM 281.5 in Dresden Island Pool, 15 miles downstream from the barrier and 55 miles from Lake Michigan, and that front has not progressed upstream since 2006. Although spawning activity has been observed in Marseilles pool in 2012, verified capture of eggs and larvae remain downstream in Peoria pool, over 140 miles from Lake Michigan (Figure 1.1.1).

Distribuição e abundância dos caranguejos Uca Leach (Crustacea, Decapoda, Ocypodidae na Baía de Guaratuba, Paraná, Brasil Distribution and abundance of fiddler crabs Uca Leach (Crustacea Decapoda Ocypodidae in Guaratuba Bay, Parana State, southern Brazil

Directory of Open Access Journals (Sweden)

Setuko Masunari

2006-12-01

Full Text Available Um estudo sobre distribuição espacial e abundância dos chama-marés Uca Leach, 1814 foi realizado na Baía de Guaratuba, Estado do Paraná. Foram coletados chama-marés de dez biótopos ao longo de um gradiente de salinidade de zero a 32 dentro da Baía de Guaratuba. Foram obtidas sete espécies, entre as quais, Uca mordax (Smith, 1870 que foi registrada somente em biótopos inundados por águas de baixas salinidades (de zero a 16. As demais espécies mostraram tolerância a uma ampla variação de salinidade, mas Uca maracoani (Latreille, 1802-1803 e Uca leptodactyla Rathbun, 1898 predominaram em águas mais salinas, de 14 a 32, enquanto U. burgersi Holthuis, 1967, Uca rapax (Smith, 1870, Uca thayeri Rathbun, 1900 e Uca uruguayensis Nobili, 1901 foram coletadas em mais de três biótopos e mostraram uma tendência ao eurihalismo, suportando salinidades de 4 a 32. Entretanto, outras características do substrato tais como porcentagem relativa de cascalho/areia/silte/argila, teor de matéria orgânica e presença de marismas, também, influenciaram a distribuição espacial destes caranguejos. U. leptodactyla foi registrada com densidade máxima de 240 ind.m-2, o mais alto valor conhecido.A study of the spatial distribution and abundance of fiddler crabs was carried out in Guaratuba Bay, Parana State, southern Brazil. Fiddler crabs were collected from 10 biotopes located along a salinity gradient from zero to 32 inside Guaratuba Bay (between 48°30'W-25°50'S and 48°45'W-25°54'S. Seven species were found, among which, Uca mordax (Smith, 1870 occurred only in biotopes inundated by low salinity water, from zero to 16. Remaining species tolerated wide range of salinity oscillation, but Uca maracoani (Latreille, 1802-1803 and Uca leptodactyla Rathbun, 1898 predominated in saltier waters, from 14 to 32, while U. burgersi Holthuis, 1967, Uca rapax (Smith, 1870, Uca thayeri Rathbun, 1900, and Uca uruguayensis Nobili, 1901 were collected in more

Experimental observations on the decay of environmental DNA from bighead and silver carps

Science.gov (United States)

Lance, Richard F.; Klymus, Katy E.; Richter, Cathy; Guan, Xin; Farrington, Heather L.; Carr, Matthew R.; Thompson, Nathan; Chapman, Duane C.; Baerwaldt, Kelly L.

2017-01-01

Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic environment. Understanding the environmental factors that influence the decay of eDNA and how these factors impact detection probabilities over time and space could have significant implications for eDNA survey design and data interpretation. Here we experimentally explore decay of eDNA associated with bighead carp (Hypophthalmichthys nobilis) biological waste collected from an aquaculture filtration system and with sperm collected from captive silver carp (H. molitrix), and how decay may be influenced by differing levels of water turbulence, temperature, microbial load, and pH. We found that the decay patterns of eDNA associated with both H. nobilis biological waste and H. molitrix milt significantly fit monophasic exponential decay curves. Secondly, we observed that the highest temperature we tested resulted in a decay half-life as much as 5.5× more rapid than the lowest temperature we tested. When we suppressed microbial loads in eDNA samples, we observed that overall losses of eDNA were reduced by about 2.5×. When we amended eDNA samples with pond water the half-life of eDNA was reduced by about 2.25×, despite relatively little apparent increase in the overall microbial load. This pattern indicated that species constituency of the microbial community, in addition to microbial load, might play a critical role in eDNA degradation. A shift in pH from 6.5 to 8.0 in the samples resulted in a 1.6× reduction in eDNA halflife. Water turbulence in our study had no apparent effect on eDNA decay. When we combined different temperature, pH, and microbial load treatments to create a

Summary of some studies on Kalmia angustifolia L.: A problem species in newfoundland forestry. Information report No. N-X-296

Energy Technology Data Exchange (ETDEWEB)

Titus, B D; Sidhu, S S; Mallik, A U

1996-12-31

The shrub known as sheep laurel or lambkill (Kalmia angustifolia L.) is commonly found as an understory component of black spruce forests, and can proliferate rapidly after stand disturbances. Subsequent regeneration and growth of spruce on Kalmia-dominated sites is often poor, and a greater understanding of the ecology of Kalmia and its interactions with black spruce is required for the management of such sites. This report reviews a series of field, greenhouse, and laboratory studies on the biology of Kalmia. It describes the phytological associations of the shrub in Newfoundland, its method of reproduction and propagation, seed germination requirements, soil requirements for adequate regeneration, and histology. Study results reported include the factors inhibiting black spruce growth by Kalmia and the response of Kalmia to control treatments. The report concludes with silvicultural recommendations to address the Kalmia problem.

Vigilance and reason - The keys to continued credibility

International Nuclear Information System (INIS)

Arlotto, G.A.

1994-01-01

I have entitle my speech open-quotes Vigilance and Reason-The Keys to Continued Credibilityclose quotes. A partitioning of the words gives insight into my view of where we are, where we may be going, and that we have control of our fate. open-quotes Continuedclose quotes indicates that I believe, at present, the American Society of Mechanical Engineers (ASME) Codes and Standards process has credibility. open-quotes Keysclose quotes connotes that we are at a crossroads and something must be done to stay on track. And open-quotes Vigilance and Reasonclose quotes suggest that we can achieve our goal of continued credibility through exercising vigilance and reason. We cannot rest on our laurels. We must take conscious, positive actions to strengthen the credibility of our products; otherwise we will backslide. It is up to us

The history of Patuxent: America’s wildlife research story

Science.gov (United States)

Perry, Matthew C.

2017-01-18

This report, based on a symposium held on October 13, 2011, at the National Wildlife Visitor Center at the Patuxent Research Refuge in Laurel, MD, documents the history of the Patuxent Research Refuge and the Patuxent Wildlife Research Center, collectively known as Patuxent. The symposium was one of the many activities occurring at that time to celebrate the 75th anniversary of the creation of the Patuxent Research Refuge in 1936. The Patuxent Wildlife Research Center is located at the refuge, and the research center director, Dr. Gregory J. Smith, with great enthusiasm, personally supervised all aspects of the celebration. The symposium was coordinated by Dr. Matthew C. Perry, the editor of this report, with Dr. Smith’s strong support. The refuge and the research center have been essentially synonymous for the almost 80 years of their history.

PerformAge

DEFF Research Database (Denmark)

Fristrup, Tine

2012-01-01

are constituted as experts on aging. In the humanistic model of aging, the aging self becomes the expert on the process of becoming of age. Thi sconflicting aspect of the discursive battlefield on aging will be elaborated in the framework of PerformAge and understood as a temporal and spatial theorising of aging......: the poetics of aging. Making the lived experience the centrepiece of this paper, poetic representations of aging lives (Laurel Richardson, 1991, 1992) will articulate subjectively felt experiences in social encounters between people performing age. In writing the social (Dorothy E. Smith, 1999) encounters, I...... as a researcher perform age in the process of figuring the possible age performativities as subject positioning performativity (Judith Butler, 1995) in the social encounters and textualized in poetic representations. Inspired by the work on Reifungsromane (Barbara Frey Waxman, 1990), I do not want to paint...

Evolutive and regressive soil sequences for characterization of soils in laurel forest (Tenerife, Canary Islands

Directory of Open Access Journals (Sweden)

José Asterio Guerra-García

2014-03-01

Full Text Available Soil degradation processes have achieved the recognition of a global environmental problem in recent years. It has been suggested by various international forums and organizations that in order to adequately establish methods to combat land degradation, it is necessary to evaluate this degradation locally and at a detailed scale. The evaluation of soil degradation of natural ecosystems at a detailed scale requires the definition of standards to which to compare this degradation. To define these standards and properly handle the processes that give rise to variations in soil quality and degradation, it is necessary to establish in some detail the pedogenic processes that have or have not taken place in a particular area and which lead to the formation of a mature soil. A mature soil should be considered as standard in these situations and, therefore, a non-degraded soil. This paper presents the possible evolutive and regressive sequences of soil, and provides some examples of using this methodology to evaluate the degradation of the same in the Monteverde of the island of Tenerife. It also presents some physical, chemical and mineralogical properties of climacic mature soils, degraded soils and low quality soils, and examines their similarities and differences in this bioclimatic environment and on different parent materials. Thus it is observed that the main processes of degradation in these areas are related to plant cover modifications that lead to the decreasing protection of the soil surface, which results in the long term, in the onset of degradation processes such as water erosion, biological degradation, loss of andic properties, compaction and sealing and crusting surface, loss of water retention capacity, illuviation, etc. Climacic soils that can be found in areas of steep lava flows are Leptosols, while gently sloping areas are Cambisols and Andosols. On pyroclastic materials there are vitric Andosols and andic Andosols according to their degree of evolution. The most characteristic regressive processes are illuviation/leaching, resulting in Luvisols, Lixisols and Alisols; and water erosion, causing the presence of secondary Leptosols.

The interaction between refugee law and international criminal justice / Fannie Lafontaine, Joseph Rikhof, Laurel Baig

Index Scriptorium Estoniae

Lafontaine, Fannie

2014-01-01

Terve ajakiri on pühendatud põgenikeõiguse ja rahvusvahelise kriminaalõiguse vahelistele seostele, sõjapõgenike kaitsele, inimkaubandusele, terrorismile ning näidetele Hollandi, Austraalia, Suurbritannia ja Kanada seadusandlusest

Microtrophic grade of wax laurel useful for recuperation of eroded soils in Tomine Embalse - Colombia

International Nuclear Information System (INIS)

Rivera, Emma L; Campos R; Yunda de, A L; Martinez R

2000-01-01

The use of the energy resources in our country should be made in an efficient way; therefore one of the principal objectives is to extend the useful of the reservoirs with reforestation programs. The use of biofertilizers is one of the biotechnological tools that could be useful for the adaptation of trees in degraded soils. Micorrhiza, a common association between roots and fungi, participate in nutrition of almost all plants. In order to study the effect of inoculation of arbuscular mycorrhizal fungus in Myrica pubescens, firstly it was carried out an evaluation of ma formed by the plant in natural conditions, and secondly, an evaluation of the effect of inoculation of the MA fungi Acaulospora longula and Glomus manihotis. Inoculation experiments were carried out in nursery conditions, in two different soils and two different nitrogen and phosphorus fertilization levels. We found structures similar to arbuscules, but the branching and diameter of intracellular hyphas were different to that of the normal arbuscules. Results showed that M. pubescens is a mycotrophic plant, but the capacity of forming mycorrhizal depending of soil conditions. M. pubescens was benefited because of inoculation of MA fungi, especially when low levels of nitrogen and phosphorus fertilization were used

Isolation of active constituents from cherry laurel (Laurocerasus officinalis Roem.) leaves through bioassay-guided procedures.

Science.gov (United States)

Akkol, Esra Küpeli; Kırmızıbekmez, Hasan; Küçükboyacı, Nurgün; Gören, Ahmet C; Yesilada, Erdem

2012-01-31

The fresh leaves of Laurocerasus officinalis Roem. (Rosaceae) are externally used against pain and feverish symptoms in Turkish folk medicine. Effects of the extracts, fractions and isolated compounds from the leaves of L. officinalis were investigated using in vivo models of inflammation and pain in mice. The crude ethanolic extract from the leaves of plant was sequentially fractionated into five subextracts; explicitly, n-hexane, chloroform, ethyl acetate (EtOAc), n-butanol, and remaining water extracts. Further studies were carried out on the most active EtOAc subextract was further subjected to fractionation through column chromatography. For the anti-inflammatory activity, carrageenan-induced hind paw edema and acetic acid-induced increase in capillary permeability models, and for the antinociceptive activity p-benzoquinone-induced writhing test in mice were employed. Ethanolic extract of the leaves was shown to possess significant inhibitory activity in the assay methods without inducing any gastric damage. Through bioassay-guided fractionation and isolation procedures three phenolic compounds, 2-O-β-D-glucopyranosyl-2-hydroxyphenyl-acetic acid (1), kaempferol-3-O-β-D-xylopyranosyl-(1→2)-O-β-D-glucopyranoside (2) and (+)-catechin (3) were isolated from the active fraction and their structures were elucidated by spectral techniques (1D and 2D NMR, ESIMS). The experimental data verified that Laurocerasus officinalis leaves displayed remarkable anti-inflammatory and antinociceptive activity. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Pollen Dispersal by Catapult: Experiments of Lyman J. Briggs on the Flower of Mountain Laurel

Science.gov (United States)

Nimmo, John R.; Hermann, Paula M.; Kirkham, M. B.; Landa, Edward R.

2014-09-01

The flower of Kalmia latifolia L. employs a catapult mechanism that flings its pollen to considerable distances. Physicist Lyman J. Briggs investigated this phenomenon in the 1950s after retiring as longtime director of the National Bureau of Standards, attempting to explain how hydromechanical effects inside the flower's stamen could make it possible. Briggs's unfinished manuscript implies that liquid under negative pressure generates stress, which, superimposed on the stress generated from the flower's growth habit, results in force adequate to propel the pollen as observed. With new data and biophysical understanding to supplement Briggs's experimental results and research notes, we show that his postulated negative-pressure mechanism did not play the exclusive and crucial role that he credited to it, though his revisited investigation sheds light on various related processes. Important issues concerning the development and reproductive function of Kalmia flowers remain unresolved, highlighting the need for further biophysical advances.

Bacteria of the genus Erwinia found in the spermatheca of the laurel psyllid Trioza alacris.

Science.gov (United States)

Marchini, Daniela; Ciolfi, Silvia; Gottardo, Marco; Marri, Laura

2014-12-01

Psylloidea are economically important insects causing serious damage to plants by direct feeding and/or vectoring bacterial pathogens. Results reported here indicate the presence of extracellular bacteria in the spermatheca of egg-laying Trioza alacris females. One phylotype, sharing 99 % identity with the non-phytopathogenic bacterium Erwinia tasmaniensis, was identified regardless of methods applied or insect sampling year and location. This is the first study, achieved by ultrastructural, cultural, and 16S rRNA gene-based analysis, of an insect spermatheca microbiota.

Juvenile pen shells (Pinna nobilis) tolerate acidification but are vulnerable to warming, supplement to: Basso, L; Hendriks, Iris; Duarte, Carlos M (2015): Juvenile pen shells (Pinna nobilis) tolerate acidification but are vulnerable to warming. Estuaries and Coasts, 38(6), 1976-1985

KAUST Repository

Basso, L; Hendriks, Iris; Duarte, Carlos M.

2016-01-01

effects of both stressors on marine invertebrates, particularly on early life stages considered more vulnerable. Disparate responses of their singular and combined effects were reported, highlighting the importance of extending the studies to different

Evaluation of leaf energy dissipation by the Photochemical Reflectance

Science.gov (United States)

Raddi, S.; Magnani, F.

studied in seedlings of 10 broadleaf tree species (Arbutus unedo, Castanea sativa, Fraxinus angustifolia, Fagussylvatica, Juglans regia, Laurus nobilis, Ligustrum vulgare, Platanus occidentalis, Quercus robur, Q. ilex, Salix capraea) under controlled conditions. To avoid the possibility of a spurious correlation in response to light, electron transport rate was modulated through changes in ambient CO2 concentration, whilst irradiance waskept constant at saturating levels. This would mimic the effects of stomatal changes under midday field conditions. Leaf photosynthetic potential (Jmax, Vcmax) and electron transport rates were derived from the resulting A/ci curves through the Farquhar model (Farquhar &von Caemmerer 1982; Farquhar, von Caemmerer &Berry 1980). Leaf reflectance in the visible region was continuously monitored with a ZEISS MCS-501 spectrometer, with a digitalisation accuracy of 16 bit, band-to-band spacing of 0.8 nm and a bandwidth of approx 3 nm (FWHM). The manipulation of ambient [CO2 ] and electron transport rate induced marked changes in leaf spectroscopy. Apart from an apparent shift in the 680-730 nm region, resulting from leaf fluorescence, a marked peak was observed at 531 nm whilst the signal at 570 nm remained almost constant after an initial acclimation to high light. The shift in leaf reflectance mirrored parallel changes in assimilation rates. As a result, a very strong correlation between PRI and computed PSII quantum efficiency was observed at the leaf level. Different leaves and species, however, differed both in the slope of the relationship and in the absolute PRI level. When all the results were pooled together, however, a significant correlation (R2 = 0.64) was still apparent. This would mimic the situation in distal remote sensing, where a variety of sunlit leaves from different species and with different photosynthetic potential would be sampled together. An even stronger correlation (R2 = 0.69) was observed, however, between PRI and

Kinnow madarin (Citrus nobilis lour × Citrus deliciosa tenora) fruit waste silage as potential feed for small ruminants.

Science.gov (United States)

Malla, B A; Rastogi, A; Sharma, R K; Ishfaq, A; Farooq, And J

2015-01-01

Study was conducted to ascertain the quality of Kinnow mandarin waste (KMW) silage and its utilization by adult male goats. KMW was collected, dried to 30% dry matter level and ensiled in silo pit after addition of disodium hydrogen orthophosphate as source of phosphorus as KMW is deficient in phosphorus. Oat was collected at milking stage, chopped finely and ensiled in a silo pit for 2 months. Twelve nondescript local adult male goats of about 8-10 months age and mean body weight of 23.00±0.90 kg were selected. The goats were randomly allotted on body weight as per randomized block design into two equal groups, six animals in each group (n=6) namely "oat silage (OS)" and "Kinnow silage." Goats were offered weighed quantities of respective silage on ad libitum basis. The silages were evaluated for proximate principles and silage quality attributes. Differences were found between chemical composition of both silages with higher organic matter, ether extracts, nitrogen free extract (p0.05 for CP) and possess comparable (2.23 vs. 2.06; p>0.05) calcium content. The pH, ammonia nitrogen (percent of total nitrogen) and soluble carbohydrate content were lower (4.20 vs. 3.30; 4.14 vs. 3.80; 2.73 vs. 1.86; p0.05) among the two dietary groups. It can be concluded that KMW can be used to prepare good quality silage for feeding of goats.

Kinnow madarin (Citrus nobilis lour × Citrus deliciosa tenora fruit waste silage as potential feed for small ruminants

Directory of Open Access Journals (Sweden)

B. A. Malla

2015-01-01

Full Text Available Aim: Study was conducted to ascertain the quality of Kinnow mandarin waste (KMW silage and its utilization by adult male goats. Materials and Methods: KMW was collected, dried to 30% dry matter level and ensiled in silo pit after addition of disodium hydrogen orthophosphate as source of phosphorus as KMW is deficient in phosphorus. Oat was collected at milking stage, chopped finely and ensiled in a silo pit for 2 months. Twelve nondescript local adult male goats of about 8-10 months age and mean body weight of 23.00±0.90 kg were selected. The goats were randomly allotted on body weight as per randomized block design into two equal groups, six animals in each group (n=6 namely “oat silage (OS” and “Kinnow silage.” Goats were offered weighed quantities of respective silage on ad libitum basis. The silages were evaluated for proximate principles and silage quality attributes. Results: Differences were found between chemical composition of both silages with higher organic matter, ether extracts, nitrogen free extract (p0.05 for CP and possess comparable (2.23 vs. 2.06; p>0.05 calcium content. The pH, ammonia nitrogen (percent of total nitrogen and soluble carbohydrate content were lower (4.20 vs. 3.30; 4.14 vs. 3.80; 2.73 vs. 1.86; p0.05 among the two dietary groups. Conclusion: It can be concluded that KMW can be used to prepare good quality silage for feeding of goats.

Effect of Ionizing irradiation on microbial load and quality and sensory characteristics of chamomile (Matricaria recutita, Anthemis nobilis)

International Nuclear Information System (INIS)

Al-Bachir, M.

2014-03-01

A study was conducted to investigate the effect of gamma irradiation and electron beam on microbial physical chemical and sensorial properties of chamomile. Powders of chamomile were exposed to 10 and 20 kGy of gamma irradiation and electron bean. Microbial load and moisture of powder, extraction rate physical chemical and sensorial properties of chamomile extract were evaluated immediately after treatment and after 12 months of storage. Results indicated that total microbial count of chamomile powder (control sample), including coliform were relatively high irradiation reduced the total count and total coliform. Electron beam was more effective in reducing the microbial load than gamma irradiation. No considerable changes in physico-chemical properties of extract produced from chamomile powder treated with low doses (10 kGy) while some changes were indicated in extract produced from chamomile powder treated with low doses (20 kGy) of gamma irradiation or electron beam. No differences were verified in the overall of sensorial (color, flavor, texture, and taste) properties of chamomile extracts after irradiation with 10 and 20 kGy of gamma irradiation or electron beam.(author)

Screening and Characterization of Polygalacturonase as Potential Enzyme for Keprok Garut Orange (Citrus nobilis var. chrysocarpa) Juice Clarification

Science.gov (United States)

Widowati, E.; Utami, R.; Kalistyatika, K.

2017-11-01

Use of thermostable enzyme from bacilli for industrial application is significant. This research aimed to isolate thermophilic pectinolytic bacteria from orange peel and vegetable waste which produced thermostable polygalacturonase, to investigate the polygalacturonase ability in clarifying keprok Garut orange juice, and to characterize polygalacturonase based on pH optimum, temperature optimum, enzyme stability, enzyme kinetics KM, and Vmax. Obtained, 14 isolates that further selected to 4 best isolates based on highest polygalacturonase activity and keprok Garut orange juice clarification ability. Four selected enzyme isolates were AR 2, AR 4, KK 4, and KK 5 had ability to increase juice transmittance, decrease juice viscosity and also reduce total soluble solid. Furthermore 4 selected isolates were partially purified by ammonium sulphate precipitation and dialysis method. Four partially purified enzymes were known that enzyme character of AR 2 optimum at pH 6; AR 4 optimum at pH 5.5; KK 4 optimum at pH 6; and KK 5 optimum at pH 4.5. Four enzymes were optimum at temperature 60°C thus stable at temperature 50-60°C, this characteristic indicate that enzymes were thermostable. AR 2 showed active activity stable at pH 4-7; AR 4 showed active activity stable at pH 6-7; KK 4 showed active activity stable at pH 4-6; however KK 5 stable at pH 4-5. Enzyme AR 2 and KK 4 was getting inactive at pH 11, thus AR 4 and KK 5 inactive at pH 12. KM value of AR 2, AR 4, KK 4, and KK 5 was 0.0959; 0.0974; 0.0966; and 0.178 mg/ml respectively. Vmax of AR 2, AR 4, KK 4, and KK 5 was 0.0203; 0.0202; 0.0185; and 0.0229 U/ml respectively. Enzyme AR 2 was the most compatible enzyme to be applied in keprok Garut orange juice clarification for it had the lowest KM value.

Off-fault ground ruptures in the Santa Cruz Mountains, California: Ridge-top spreading versus tectonic extension during the 1989 Loma Prieta earthquake

Science.gov (United States)

Ponti, Daniel J.; Wells, Ray E.

1991-01-01

The Ms 7.1 Loma Prieta earthquake of 18 October 1989 produced abundant ground ruptures in an 8 by 4 km area along Summit Road and Skyland Ridge in the Santa Cruz Mountains. Predominantly extensional fissures formed a left-stepping, crudely en echelon pattern along ridges of the hanging-wall block southwest of the San Andreas fault, about 12 km northwest of the epicenter. The fissures are subparallel to the San Andreas fault and appear to be controlled by bedding planes, faults, joints, and other weak zones in the underlying Tertiary sedimentary strata of the hanging-wall block. The pattern of extensional fissures is generally consistent with tectonic extension across the crest of the uplifted hanging-wall block. Also, many displacements in Laurel Creek canyon and along the San Andreas and Sargent faults are consistent with right-lateral reverse faulting inferred for the mainshock. Additional small tensile failures along the axis of the Laurel anticline may reflect growth of the fold during deep-seated compression. However, the larger ridge-top fissures commonly have displacements that are parallel to the north-northeast regional slope directions and appear inconsistent with east-northeast extension expected from this earthquake. Measured cumulative displacements across the ridge crests are at least 35 times larger than that predicted by the geodetically determined surface deformation. These fissures also occur in association with ubiquitous landslide complexes that were reactivated by the earthquake to produce the largest concentration of co-seismic slope failures in the epicentral region. The anomalously large displacements and the apparent slope control of the geometry and displacement of many co-seismic surface ruptures lead us to conclude that gravity is an important driving force in the formation of the ridge-top fissures. Shaking-induced gravitational spreading of ridges and downslope movement may account for 90¿ or more of the observed displacements on

MARINATED WITH EFFECT OF NaCl AND SODIUM TRIPOLYPHOSPHATE BROMATOLOGICAL PROPERTIES ON THE MEAT GOAT

Directory of Open Access Journals (Sweden)

Ricardo Peña F.

2014-12-01

Full Text Available Goat meat has all the significant nutritional characteristics by consumers to be an excellent alternative supply; therefore, this study proposes the use of a mixture of NaCl, sodium tripolyphosphate, laurel and rosemary extracts as marginalisers agents using maceration technique marinade as facilitator system and applying vacuum packaging and storage at refrigeration of an overall strategy of modernization. In the finished product bromatologic variables pH, fat, protein, water holding capacity, tenderness and moisture during the days 0, 7, 14, 21 and 28 were evaluated in chilled storage. The results showed that the treatment is marinated improves water retention, protein retention and raises the pH of the product from the first day of storage because we can say that the application of the marinating process by mashing influences bromatological significantly on properties of meat goat breed Santander , becoming an alternative to industrialization

1,8-cineole protected human lipoproteins from modification by oxidation and glycation and exhibited serum lipid-lowering and anti-inflammatory activity in zebrafish

Directory of Open Access Journals (Sweden)

Kyung-Hyun Cho1,2,*

2012-10-01

Full Text Available We recently reported that a water extract of laurel or turmeric,1,8-cineole enriched fractions, showed hypolipidemic activityin the zebrafish model. Therefore, the present study investigatedthe cineole’s anti-oxidant and anti-inflammatory activitiesin lipoprotein metabolism in vitro and in vivo. Cineolehad inhibitory effects on cupric ion-mediated oxidation of lipoproteinsin general, while simultaneously enhancing ferric ionremoval ability in high-density lipoprotein (HDL. Hypercholesterolemiawas induced in zebrafish using cholesterol-feedingtreatment, 4% cholesterol, for 3 weeks. After feeding with orwithout the addition of cineole, the results revealed that cineolepossessed lipid-lowering and anti-inflammatory activitiesin hypercholesterolemic zebrafish. In addition, serum amyloidA and interleukin-6 levels were lowered and lipid accumulationwas decreased in the liver. Conclusively, 1,8-cineole wasfound to have anti-oxidant activities in lipoprotein metabolismboth in vitro and in vivo with simultaneous reduction of lipidaccumulation in the liver of zebrafish.

Plasma and the universe

International Nuclear Information System (INIS)

Falthammar, C.G.

1988-01-01

Hannes Alfven has enjoyed a long and spectacular career in which he has time and again pioneered the path that other workers were able fruitfully to exploit. One thinks of the Alven waves, the concept of gyrocenter drift and the perturbation theory based on it, and the critical velocity phenomenon in the interaction of a plasma with a neutral gas. All of these discoveries have been of fundamental importance. By no means content to rest on his laurels, Alfven has, during the last decade, contributed to science in a major sense, especially in the field of cosmogeny. For example, he has used the latest data from the Voyager spacecraft to test his detailed predictions of the structure of the Saturnian rings. Alfven's current preoccupation is with the Plasma Universe and, as may be expected, some of his concepts are receiving observational support, while others are still controversial

Ragusan merchants and the Crusades in the Late Middle Ages. Financial support for the war and bequests pro anima... “ad pasagium turchorum seu saracenorum, et alliud super patarenos Bosnie”

Directory of Open Access Journals (Sweden)

Romano, Daniela

2008-12-01

Full Text Available Ragusan wills of 13th and 14th centuries reveal peculiar and unsuspected aspects about armed pilgrimages by proxy in the Balkans. The struggle against infedeles and heretics propagandized by the Roman Church finds financial support and full agreement in Dubrovnik citizens, also thanks to the Franciscan and Dominican preaching. Nobles, merchants, clerics and priests, craftsmen, men and women, they are all ready to take part in the Just war. Anyway they would fight only on the condition of leaving a sum of money to those who would fight for their souls’ salvation.

I testamenti ragusei del XIII e XIV secolo rivelano aspetti particolari e per nulla indagati sul pellegrinaggio armato per procura in area balcanica. La lotta agli infedeles e agli eretici messa in atto dalla Chiesa di Roma trova sostegno economico e piena adesione nei cittadini di Dubrovnik, città-stato baluardo della cristianità più a levante, anche grazie alla campagna pubblicistica attuata da francescani e domenicani. Nobili, mercanti, chierici e preti, artigiani, uomini e donne partecipano alla giusta guerra, prevedendo lasciti per uomini che combatteranno per la salvezza delle anime dei testatori.

Annotated type catalogue of the Orthalicoidea (Mollusca, Gastropoda) in the Museum für Naturkunde, Berlin.

Science.gov (United States)

Breure, Abraham S H

2013-01-01

The type status is described of 96 taxa classified within the superfamily Orthalicoidea and present in the Mollusca collection of the Museum für Naturkunde der Humboldt-Universität zu Berlin. Lectotypes are designated for the following taxa: Orthalicus elegans Rolle, 1895; Bulimus maranhonensis Albers, 1854; Orthalicus nobilis Rolle, 1895; Orthalichus tricinctus Martens, 1893. Orthalicus sphinx tresmariae is introduced as new name for Zebra sphinx turrita Strebel, 1909, not Zebra quagga turrita Strebel, 1909. The following synonyms are established: Zebra crosseifischeri Strebel, 1909 = Orthalicus princeps fischeri Martens, 1893; Orthalicus isabellinus Martens, 1873 = Orthalicus bensoni (Reeve, 1849); Zebra zoniferus naesiotes Strebel, 1909 = Orthalicus undatus (Bruguière, 1789); Porphyrobaphe (Myiorthalicus) dennisoni pallida Strebel, 1909 = Hemibulimus dennisoni (Reeve, 1848); Zebra delphinus pumilio Strebel, 1909 = Orthalicus delphinus (Strebel, 1909); Orthalicus (Laeorthalicus) reginaeformis Strebel, 1909 = Corona perversa (Swainson, 1821); Bulimus (Eurytus) corticosus Sowerby III, 1895 = Plekocheilus (Eurytus) stuebeli Martens, 1885. The taxon Bulimus (Eudioptus) psidii Martens, 1877 is now placed within the family Sagdidae, tentatively in the genus Platysuccinea. Appendices are included with an index to all the types of Orthalicoidea extant (including those listed by Köhler 2007) and a partial list of letters present in the correspondence archives.

Antibacterial and Antifungal Activity of Essential Oils against Pathogens Responsible for Otitis Externa in Dogs and Cats.

Science.gov (United States)

Ebani, Valentina V; Nardoni, Simona; Bertelloni, Fabrizio; Najar, Basma; Pistelli, Luisa; Mancianti, Francesca

2017-04-21

Background: Essential oils (EOs) are recommended by some veterinarians to treat otitis externa in pets, but data about their efficacy in scientific literature are very scant. Methods: Nine commercial EOs, from roman chamomile ( Anthemis nobilis L.), star anise ( Illicium verum ), lavender ( Lavandula hybrida ), litsea ( Litsea cubeba (Lour.) Pers.), basil ( Ocimum basilicum L.), oregano ( Origanum vulgare L. subsp. hirticum ), rosemary ( Rosmarinus officinalis L.), clary sage ( Salvia sclarea L.), and thyme ( Thymus vulgaris L.) were tested against bacterial and fungal pathogens previously isolated from dogs and cats with otitis externa. In particular, the analyses were carried out against Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus pseudointermedius , Aspergillus niger, Aspergillus fumigatus, Aspergillus terreus, Candida albicans, Candida tropicalis, Trichosporon sp., and Rhodotorula sp. Results: O. vulgare and S. sclarea showed superior antibacterial activity, even if not against all the strains. Trichosporon sp., C. albicans , and A. terreus were insensitive to most Eos, while other yeasts and molds showed different degrees of sensitivity. In particular, most fungi were inhibited by O. vulgare and R. officinalis . Conclusions: The obtained results suggest that some EOs could be included in treatment as an alternative therapeutic option in bacterial otitis complicated by fungi, in association with conventional drugs.

A study on the relationship of arsenic accumulation with protein, lipid, ash and moisture contents in muscle of eight species of fish in Iran

Directory of Open Access Journals (Sweden)

A Askary Sary

2012-11-01

Full Text Available A comparative study was conducted to investigate a relationship between concentration of arsenic with protein, lipid, ash and moisture content in Cyprinus carpio, Oncorhynchus mykiss, Aristichthys nobilis, Hypophthalmichthys molitrix, Ctenopharyngodon idella, Scomberomorus commerson, Scomberomorus guttatus and Otolithes ruber. A total of 72 sample of common carp, Bighead carp, silver carp and grass carp fishing from Azadegan fish farming center, Ahvaz; Rainbow trout from Cheshme Dimeh and Scomberomorus commerson, Scomberomorus guttatus and Otolithes ruber caught with gill netfrom Hendijan. Wet-digestion method was performed prior to arsenic determination in the samples. The level of arsenic was measured by atomic absorption spectrophotometer. The results showed that concentration of arsenic in the muscle of fishes was 269.87 ± 20.96 µg/Kg. Moreover, levels of protein, lipid, ash and moisture in the samples were estimated at 19.67±0.78 g/100, 2.45±0.45 g/100, 1.49±0.23 g/100, 78±1.89 g/100, respectively. Results also showed a positive correlation between the accumulation of arsenic in muscle of fishes with levels of protein, lipid, ash and moisture (p

La prolusione di rocco e le dottrine del processo penale

Directory of Open Access Journals (Sweden)

Renzo Orlandi

2015-03-01

Full Text Available L’articolo analizza le conseguenze del pensiero giuridico di Arturo Rocco, dal 1910 nella dottrina di procedura penale, ossia, il metodo tecnico-giuridico (esegesi, sistematica e critica. La dogmatica di procedura pena- le inizia con la monografia di Giovanni Conso, nel 1955. L’insoddisfazione con il tecnicismo giuridico e con la mancanza di maturità scientifica si è manifestata con Carnelutti nel 1946 (Cerenterola, sottolineando le peculiarità strutturali del procedimento penale. In questa prospettiva, il testo sottolinea la conferenza di Franco Cordero nel 1964 (Lecce, così come le lezioni da James Goldschmidt. Inoltre, negli anni sessanta, divennero importanti diritti fondamentali, di fronte alla Costituzione democratica, con un nuovo orientamento dottrinale. Il diritto processuale penale è stato considerato come diritto costituzionale applicato. Si segnalano gli insegnamenti di Amodio, Amato, Chiavario, Ferrua, Grevi, Iluminati, Massa e Nobili. Successivamente, l’articolo mette in evidenza il CPP del 1988, l’internazionalizzazione dei sistemi giuridici, l’importanza del diritto comparato e dei diritti fondamentali. Si conclude con il nuovo ordine mondiale dei diplomi internazionali e tribunali sovranazionali, con nuove esigenze, oltre il tecnicismo giuridico.

Interaktywna prezentacja zagadnień środowiskowych za pomocą Google Fusion Tables na przykładzie zdjęć fitosocjologicznych wybranych okolic Tenczynka

Directory of Open Access Journals (Sweden)

Karol Król

2017-09-01

Full Text Available W ostatnich latach zaobserwować można dynamiczny rozwój interaktywnych form przekazu, w tym kartografii internetowej i geowizualizacji. Celem pracy jest charakterystyka i ocena wybranych technik i narzędzi komputerowych wykorzystanych do utworzenia interaktywnej mapy zjawisk przestrzennych prezentującej wyniki obserwacji florystycznych przeprowadzonych w sołectwie Tenczynek (woj. małopolskie. Na badanym obszarze zidentyfikowano dwa szczególnie cenne przyrodniczo gatunki: kłokoczki południowej (Staphylea pinnata L., krzewu spotykanego jedynie w południowej części Polski, w żyznych lasach liściastych oraz przylaszczki pospolitej (Hepatica nobilis Schreb., gatunku wskaźnikowego klasy Querco-Fagetea obszarów najcieplejszych. W konkluzji wykazano, że narzędzie Fusion Tables jest użyteczne i stosunkowo proste w obsłudze przy relatywnie niewielkich nakładach pracy pozwala utworzyć interaktywną mapę dowolnego zjawiska o charakterze przestrzennym. Zwrócono również uwagę, że aplikacje internetowe mogą pełnić funkcję informacyjno-edukacyjną poprzez ukierunkowany i odpowiednio zredagowany przekaz.

Screening by coral green fluorescent protein (GFP)-like chromoproteins supports a role in photoprotection of zooxanthellae

Science.gov (United States)

Smith, E. G.; D'Angelo, C.; Salih, A.; Wiedenmann, J.

2013-06-01

Green fluorescent protein (GFP)-like pigments are responsible for the vivid colouration of many reef-building corals and have been proposed to act as photoprotectants. Their role remains controversial because the functional mechanism has not been elucidated. We provide direct evidence to support a photoprotective role of the non-fluorescent chromoproteins (CPs) that form a biochemically and photophysically distinct group of GFP-like proteins. Based on observations of Acropora nobilis from the Great Barrier Reef, we explored the photoprotective role of CPs by analysing five coral species under controlled conditions. In vitro and in hospite analyses of chlorophyll excitation demonstrate that screening by CPs leads to a reduction in chlorophyll excitation corresponding to the spectral properties of the specific CPs present in the coral tissues. Between 562 and 586 nm, the CPs maximal absorption range, there was an up to 50 % reduction of chlorophyll excitation. The screening was consistent for established and regenerating tissue and amongst symbiont clades A, C and D. Moreover, among two differently pigmented morphs of Acropora valida grown under identical light conditions and hosting subclade type C3 symbionts, high CP expression correlated with reduced photodamage under acute light stress.

Susceptibility to Phytophthora ramorum in California bay laurel, a key foliar host of sudden oak death

Science.gov (United States)

Brian L. Anacker; Nathan E. Rank; Daniel Hüberli; Matteo Garbelotto; Sarah Gordon; Rich Whitkus; Tami Harnik; Matthew Meshriy; Lori Miles; Ross K. Meentemeyer

2008-01-01

Sudden oak death, caused by the water mold Phytophthora ramorum, is a plant disease responsible for the death of hundreds of thousands of oak and tanoak trees. Some foliar hosts play a major role in the epidemiology of this disease. Upon infection by P. ramorum, these foliar hosts express non-fatal leaf lesions from which large...

Resting on Laurels: The Effects of Discrete Progress Markers as Subgoals on Task Performance and Preferences

Science.gov (United States)

Amir, On; Ariely, Dan

2008-01-01

This article investigates the influence of progress certainty and discrete progress markers (DPMs) on performance and preferences. The authors suggest that the effects of DPMs depend on whether progress certainty is high or low. When the distance to the goal is uncertain, DPMs can help reduce uncertainty and thus improve performance and increase…

The State of Integrated Air and Missile Defense Held in Laurel, Maryland on July 14, 2011

Science.gov (United States)

2011-07-14

compromised from servers (-22%) 86% were discovered by a third party (+25%) 96% of breaches were avoidable (+-0) Source 2011 Data Breach Investigations...Foreign Espionage - Terrorists - State Sponsored Attacks UNCLASSIFIED UNCLASSIFIED 11 What commonalities exist? How do breaches occur? Verizon Data ... Breach Study “Breaching organizations still doesn’t typically require highly sophisticated attacks, most victims are a target of opportunity rather

Pathogenicity variation in two west coast forest Phytophthoras, Phytophthora nemorosa and P. pseudosyringae, to bay laurel

Science.gov (United States)

R.E. Linzer; M. Garbelotto

2008-01-01

Two recently described pathogenic oomycetes, Phytophthora nemorosa and P. pseudosyringae, have overlapping host and geographic ranges in California and Oregon forests with P. ramorum, causal agent of ?sudden oak death? disease. Preliminary genetic evidence indicates P. nemorosa and P....

AFSC/RACE/FBEP/Laurel: Substrate preference and delayed settlement in northern rock sole larvae Lepidopsetta polyxystra

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This dataset is from laboratory experiments testing the onset and ontogenetic changes in habitat selection of pre- and post-settling northern rock sole (NRS) larvae,...

Estructura del aparato reproductor masculino de Uca uruguayensis (Decapoda, Ocypodidae Structure of the male reproductive tract of Uca uruguayensis (Decapoda, Ocypodidae

Directory of Open Access Journals (Sweden)

Elena Irene Cuartas

Full Text Available El objetivo de este trabajo es describir las estructuras del sistema reproductor de machos de Uca uruguayensis Nobili, 1901 y estudiar los cambios relacionados con la madurez sexual. Se trabajó con observación estereoscópica de material fresco, técnica histológica de rutina y observaciones en Microscopio Electrónico de Barrido. Se incluyó la caracterización de los gonopodos 1 (G1 y 2 (G2. El tracto reproductor del macho se compone de un par de testículos (T, un vaso deferente (VD tubular y sinuoso y una ampolla terminal (AT. El VD tiene tres secciones, la anterior (VDA, la media (VDM y la posterior (VDP. El VDA y VDM están conformadas por un epitelio simple de células cúbicas. El epitelio del VDP es columnar y con núcleos basales y alongados. El diseño tubular se modifica al ocuparse el lumen del VDP con líquido espermático durante el verano y la musculatura circular se hace más evidente. La porción terminal del VDP se ensancha formando una ampolla (AT que comprende cuatro cámaras interconectadas. Todas las estructuras están rodeadas de una capa de tejido conectivo de poco espesor. Se identifican las modificaciones observadas en la histología de T y VD, definiendo como mas relevantes las observadas desde el mes de noviembre hasta marzo. Estas modificaciones sugieren que U. uruguayensis tiene, en la localidad estudiada, una única estación reproductiva durante el verano en esta latitud. La AT, tal como es descripta, es una estructura que hasta el momento no ha sido mencionada para los Brachyura.The structure of the male reproductive tract was described in Uca uruguayensis Nobili, 1901, by using histological methods, scanning electron microscopy techniques, and stereoscopic observations of fresh material. The aim of this work was to establish the functional changes associated with sexual maturation. The morphology of the first (G1 and second (G2 pair of gonopods was described. The male reproductive tract consists of paired

In situ studies on the distribution patterns and dynamics of microcystins in a biomanipulation fish - bighead carp (Aristichthys nobilis)

International Nuclear Information System (INIS)

Chen Jun; Xie Ping; Zhang Dawen; Lei Hehua

2007-01-01

The distribution and dynamics of microcystins in various organs of the phytoplanktivorous bighead carp were studied monthly in Lake Taihu, which is dominated by toxic cyanobacteria. There was a good agreement between LC-MS and HPLC-UV determinations. Average recoveries of spiked fish samples were 63% for MC-RR and 71% for MC-LR. The highest MC contents in intestine, liver, kidney and spleen were 85.67, 2.83, 1.70 and 1.57 μg g -1 DW, respectively. MCs were much higher in mid-gut walls (1.22 μg g -1 DW) than in hind- and fore-gut walls (0.31 and 0.18 μg g -1 DW, respectively), suggesting the importance of mid-gut wall as major site for MC absorption. A cysteine conjugate of MC-LR was detected frequently in kidney. Among the muscle samples analyzed, 25% were above the provisional tolerable daily intake level by WHO. Bighead is strongly resistant to microcystins and can be used as biomanipulation fish to counteract cyanotoxin contamination in eutrophic waters. - Bighead carp is resistant to microcystins and can be used as biomanipulation fish to counteract cyanotoxin contamination

Applying Hotspot Detection Methods in Forestry: A Case Study of Chestnut Oak Regeneration

International Nuclear Information System (INIS)

Fei, S.

2010-01-01

Hotspot detection has been widely adopted in health sciences for disease surveillance, but rarely in natural resource disciplines. In this paper, two spatial scan statistics (SaT Scan and Cluster Seer) and a non spatial classification and regression trees method were evaluated as techniques for identifying chestnut oak (Quercus Montana) regeneration hotspots among 50 mixed-oak stands in the central Appalachian region of the eastern United States. Hotspots defined by the three methods had a moderate level of conformity and revealed similar chestnut oak regeneration site affinity. Chestnut oak regeneration hotspots were positively associated with the abundance of chestnut oak trees in the over story and a moderate cover of heather species (Vaccinium and Gaylussacia spp.) but were negatively associated with the abundance of hay scented fern (Dennstaedtia punctilobula) and mountain laurel (Kalmia latiforia). In general, hotspot detection is a viable tool for assisting natural resource managers with identifying areas possessing significantly high or low tree regeneration.

Applying Hotspot Detection Methods in Forestry: A Case Study of Chestnut Oak Regeneration

Directory of Open Access Journals (Sweden)

Songlin Fei

2010-01-01

Full Text Available Hotspot detection has been widely adopted in health sciences for disease surveillance, but rarely in natural resource disciplines. In this paper, two spatial scan statistics (SaTScan and ClusterSeer and a nonspatial classification and regression trees method were evaluated as techniques for identifying chestnut oak (Quercus Montana regeneration hotspots among 50 mixed-oak stands in the central Appalachian region of the eastern United States. Hotspots defined by the three methods had a moderate level of conformity and revealed similar chestnut oak regeneration site affinity. Chestnut oak regeneration hotspots were positively associated with the abundance of chestnut oak trees in the overstory and a moderate cover of heather species (Vaccinium and Gaylussacia spp. but were negatively associated with the abundance of hayscented fern (Dennstaedtia punctilobula and mountain laurel (Kalmia latiforia. In general, hotspot detection is a viable tool for assisting natural resource managers with identifying areas possessing significantly high or low tree regeneration.

Lysophosphatidic Acid Acyltransferase from Coconut Endosperm Mediates the Insertion of Laurate at the sn-2 Position of Triacylglycerols in Lauric Rapeseed Oil and Can Increase Total Laurate Levels

Science.gov (United States)

Knutzon, Deborah S.; Hayes, Thomas R.; Wyrick, Annette; Xiong, Hui; Maelor Davies, H.; Voelker, Toni A.

1999-01-01

Expression of a California bay laurel (Umbellularia californica) 12:0-acyl-carrier protein thioesterase, bay thioesterase (BTE), in developing seeds of oilseed rape (Brassica napus) led to the production of oils containing up to 50% laurate. In these BTE oils, laurate is found almost exclusively at the sn-1 and sn-3 positions of the triacylglycerols (T.A. Voelker, T.R. Hayes, A.C. Cranmer, H.M. Davies [1996] Plant J 9: 229–241). Coexpression of a coconut (Cocos nucifera) 12:0-coenzyme A-preferring lysophosphatitic acid acyltransferase (D.S. Knutzon, K.D. Lardizabal, J.S. Nelsen, J.L. Bleibaum, H.M. Davies, J.G. Metz [1995] Plant Physiol 109: 999–1006) in BTE oilseed rape seeds facilitates efficient laurate deposition at the sn-2 position, resulting in the acccumulation of trilaurin. The introduction of the coconut protein into BTE oilseed rape lines with laurate above 50 mol % further increases total laurate levels. PMID:10398708

Evaluation of nitrogen sources (15 N) on three wheat varieties in an andisol and an ultisol in the IX Region. II: Isotopic parameters and fertilizer use efficiency

International Nuclear Information System (INIS)

Pino N, Ines; Buneder B, Mirta; Peyrelongue C, Amelia

1996-01-01

A field study was carried out in order to evaluate different N fertilizers sources in three wheat varieties considering an Andisol and an Ultisol soils, in the IX Region of the country. The dilution isotopic techniques was used, with AS, 10% at rates of 20 kg ha -1 of N. The isotopic parameters such as N in the plant derived from the nitrogen sources, the N in the plant derived from the soil, the fertilizer use efficiencies and the agronomic evaluation between them were determined. The Nddfu (%) was associated to the varieties and to the soils. In the Ultisol, Dalcahue variety had a better behaviour with SS and, in the Andisol, Laurel variety showed an special affinity with U. In three varieties, the higher % of N derived from the sources it was in the grain, showing Dalcahue variety a better translocation.The fertilizer use efficiency (FUE) and the physiological efficiency, determined according the isotopic parameters, were higher than the values determined according the conventional methodology. (author)

Separation of uranium(V I) from binary solution mixtures with thorium(IV), zirconium(IV) and cerium(III) by foaming

International Nuclear Information System (INIS)

Shakir, K.; Aziz, M.; Benyamin, K.

1992-01-01

Foam separation has been investigated for the removal of uranium(V I), thorium(IV), zirconium(IV) and cerium(III) from dilute aqueous solutions at pH values ranging from about I to about II. Sodium laurel sulphate (Na L S) and acetyl trimethyl ammonium bromide (CTAB), being a strong anionic and a strong cationic surfactants, were used as collectors. The results indicate that Na L S can efficiently remove thorium(IV), zirconium(IV) and cerium(III) but not uranium(V I). CTAB, on the other hand, can successfully float only uranium(V I) and zirconium(IV). These differences in flotation properties of the different cations could be used to establish methods for the separation of uranium(V I) from binary mixtures with thorium(IV), zirconium(IV) or cerium(III). The results are discussed in terms of the hydrolytic behaviour of the tested cations and properties of used collectors.2 fig., 1 tab

Regional distribution of native and exotic species in levees of the lower delta of the Paraná river - DOI: 10.4025/actascibiolsci.v30i4.5869

Directory of Open Access Journals (Sweden)

Pablo Aceñolaza

2008-11-01

Full Text Available The distribution and abundance of exotic and native species in levee neo-ecosystems were analyzed. No invasive species were found in unit A; their absence could be explained by the fluvial action of the Paraná river, extraordinary flood episodes and anthropic disturbances. Invasive species associated with the tidal regimen of the de la Plata river were present in units B and C, particularly Chinese privet (L. sinense, green ash (F. pennsylvanica, honey locust (G. triacanthos, Japanese honeysuckle (L. Japonica, blackberry ( Rubus spp., box elder (A. Negundo and glossy privet (L. Lucidum.Native species showed low recovery values, both at a regional level and within each unit, with the exception of A. The neo-ecosystems with the greater degree of abandonment of units B and C exhibited dominance of exotic tree species and, to a lesser extent, recovery of native species of the original gallery forest (seibo, Erythrina crista galli; laurel, Nectandra falcifolia; canelón, Rapanea spp. and arrayán, Blepharocalyx tweediei.

Evaluation of nitrogen sources (15 N) on three wheat varieties in an andisol and an ultisol, in the IX region. 2. Isotopic parameters and fertilizer use efficiency

International Nuclear Information System (INIS)

Pino N, Ines; Peyrelongue C, A.; Buneder B, Mirta

1997-01-01

Full text: A field study was carried out in order to evaluate different N fertilizers sources un three wheat varieties, considering an Andisol and Ultisol soils, in the IX Region of the country. The dilution isotopic techniques was used, with As, 10% at rates of 20 kg ha -1 of N. The isotopic parameters such as N in the plant derived from the nitrogen sources, the N in the plant derived from the soil, the fertilizer use efficiencies and the agronomic evaluation between them were determined. The Nddfu (%) was associated to the varieties and to the soils. In the Ultisol, Dalcahue variety had a better behaviour with SS and, in the Andisol, Laurel variety showed an special affinity with U. In the three varieties, the higher % of N derived from the sources it was in the grain, showing Dalcahue variety a better translocation. The fertilizer use efficiency (FUE) and the physiological efficiency, determined according the isotopic parameters, were higher than the values determined according the conventional methodology

Radioimmunoassay of alpha-foeto-protein in hepatology. Observation of 151 cases

International Nuclear Information System (INIS)

Dao, Mouhamadou.

1976-03-01

Alpha-foeto-protein, although discovered several years ago, was considered as only able to exist in primary adult liver cancer. With progress in science and in research it was observed that AFP can be found in normal subjects, without any pathological significance. APF has been found moreover in illnesses other than primary liver cancer. This range of diseases, restricted at first, has continued to widen, a development due to the discovery of new AFP detection methods and radioimmunoassay. Our work, covering 151 cases discussed in detail in the 'Material and Methods' chapter, is interesting from two viewpoints: in hepatology it is useful to know whether the very sensitive radio-immunological method reveals abnormal AFP content variations in different liver diseases; given the large number of hepatomas in our series it is also interesting to have the value of this determination confirmed in primary liver cancer diagnosis. Radioimmunoassay now replaces other quantitative methods (MANCINI's radial immunodiffusion and LAURELL's elec. diffusion) and is the most accurate [fr