IL1RN and KRT13 Expression in Bladder Cancer: Association with Pathologic Characteristics and Smoking Status

Directory of Open Access Journals (Sweden)

Thomas S. Worst

2014-01-01

Full Text Available Purpose. To validate microarray data on cytokeratin 13 (KRT13 and interleukin-1 receptor antagonist (IL1RN expression in urothelial carcinoma of the urinary bladder (UCB and to correlate our findings with pathologic characteristics and tobacco smoking. Methods. UCB tissue samples (n=109 and control samples (n=14 were obtained from transurethral resection and radical cystectomy specimens. Immunohistochemical staining of KRT13 and IL1RN was performed and semiquantitative expression scores were assessed. Smoking status was evaluated using a standardized questionnaire. Expression scores were correlated with pathologic characteristics (tumor stage and grade and with smoking status. Results. Loss of KRT13 and IL1RN expression was observed in UCB tissue samples when compared to controls (P=0.007, P=0.008 in which KRT13 and IL1RN expression were high. IL1RN expression was significantly reduced in muscle-invasive tumors (P=0.003. In tissue samples of current smokers, a significant downregulation of IL1RN was found when compared to never smokers (P=0.013. Conclusion. Decreased expressions of KRT13 and IL1RN are common features of UCB and are associated with aggressive disease. Tobacco smoking may enhance the loss of IL1RN, indicating an overweight of proinflammatory mediators involved in UCB progression. Further validation of the influence of smoking on IL1RN expression is warranted.

Meelis Virkebau : Kreenholm peaks meediaga suhtlema / Tanel Mazur

Index Scriptorium Estoniae

Mazur, Tanel, 1971-

2007-01-01

Ilmunud ka: Narva Postiljon, 5. apr. 2007, lk. 1,2; Narvskaja Gazeta, 5. apr. 2007, lk. 1,2. Kreenholmi endine juht Meelis Virkebau ei mõista Narva linnapea Tarmo Tammiste seisukohta, et 3000 töötut ei ole linnale probleem, samuti peab ta mõtlematuks peaminister Andrus Ansipi väidet, et Kreenholmi probleem pole nii hull kui paistab. M. Virkebau selgitab oma lahkumist ettevõtte juhi kohalt 2003. aastal

Pangad nopivad odava kinnisvara / Birjo Must ; kommenteerinud Hannes Kuhlbach, Meelis Šokman

Index Scriptorium Estoniae

Must, Birjo

2010-01-01

Võrreldes 2010. a. algusega on SEB makseraskustes klientidelt ostetud kinnisvara mahu neljakordistanud, Swedbank on mahu enam kui kahekordistanud. Kommenteerivad SEB kinnisvarahaldusfirma Estectus OÜ juht Hannes Kuhlbach ja Swedbanki Eesti kinnisvarahalduse ettevõtte Ektornet Residential Estonia OÜ juht Meelis Šokman

Meeli Kõiva vitraažid ajakirja Stained Glass talvenumbris / Rein Eriksson

Index Scriptorium Estoniae

Eriksson, Rein

1998-01-01

Intervjuust Meeli Kõivaga Ameerika Ühendriikide vitraažikunstnike Assotsiatsiooni ajakirja "Stained Glass" talvenumbris. Avaldati 8 fotot kunstniku vitraažidest. Artikli "Unistused transparentsest ruumist" autor - USA vitraažikunstnike Assotsiatsiooni endine president Helene Weiss. M. Kõiva esines aprillis 1997 akvarellidega grupinäitusel New Yorgis, Broadway galeriis Art 54.

Klaas on kaasaegne ja vastupidav materjal : skulptuur / Meeli Kõiva ; interv. Reet Varblane

Index Scriptorium Estoniae

Kõiva, Meeli

2007-01-01

Klaasikunstnik Meeli Kõiva tutvustas Tamperes klaasifoorumil "Glass Performance Days 2007" oma klaasvalgusskulptuure. Ettekanne valmis koostöös Aulis Bertiniga. Arhitektuursest klaasikunstist, koostööst Ekku Peltomäkiga, Eestis teostamata jäänud klaas- ja valgusskulptuuridest, kunstist, mida 1% ulatuses kogu hoone maksumusest rahastatakse

OT-Combiners Via Secure Computation

DEFF Research Database (Denmark)

Harnik, Danny; Ishai, Yuval; Kushilevitz, Eyal

2008-01-01

of faulty candidates (t = Ω(n)). Previous OT-combiners required either ω(n) or poly(k) calls to the n candidates, where k is a security parameter, and produced only a single secure OT. We demonstrate the usefulness of the latter result by presenting several applications that are of independent interest......An OT-combiner implements a secure oblivious transfer (OT) protocol using oracle access to n OT-candidates of which at most t may be faulty. We introduce a new general approach for combining OTs by making a simple and modular use of protocols for secure computation. Specifically, we obtain an OT......, strengthen the security, and improve the efficiency of previous OT-combiners. In particular, we obtain the first constant-rate OT-combiners in which the number of secure OTs being produced is a constant fraction of the total number of calls to the OT-candidates, while still tolerating a constant fraction...

"Tallinn Treff" - maailma lavade uus põlvkond Tallinnas / Meelis Pai, Vahur Keller, Reeda Toots ; intervjueerinud Margot Visnap

Index Scriptorium Estoniae

Pai, Meelis, 1968-

2009-01-01

30. maist 6. juunini toimuvast rahvusvahelisest nukuteatrifestivalist "Tallinn Treff" ning selle alafestivalist "Noor vaim" annavad ülevaate festivali peakorraldaja Meelis Pai, kusntiline juht Vahut Keller ja "Noor vaimu" juht Reeda Toots

The glossopharyngeal nerve controls epithelial expression of Sprr2a and Krt13 around taste buds in the circumvallate papilla.

Science.gov (United States)

Miura, Hirohito; Kusakabe, Yuko; Hashido, Kento; Hino, Akihiro; Ooki, Makoto; Harada, Shuitsu

2014-09-19

Tastants reach the tip of taste bud cells through taste pores which are openings in the epithelium. We found Sprr2a is selectively expressed in the upper layer of the epithelium surrounding taste buds in the circumvallate papilla (CV) where the epithelium is organized into taste pores. Sprr2a is a member of a small proline-rich protein family, which is suggested to be involved in the restitution/migration phase of epithelial wound healing. The expression of Sprr2a was restricted to the upper layer and largely segregated with Ptch1 expression that is restricted to the basal side of the epithelium around the taste buds. Denervation resulted in the gradual loss of Sprr2a-expressing cells over 10 days similarly to that of taste bud cells which is in contrast to the rapid loss of Ptch1 expression. We also found that denervation caused an increase of Keratin (Krt)13 expression around taste buds that corresponded with the disappearance of Sprr2a and Ptch1 expression. Taste buds were surrounded by Krt13-negative cells in the CV in control mice. However, at 6 days post-denervation, taste buds were tightly surrounded by Krt13-positive cells. During taste bud development, taste bud cells emerged together with Krt13-negtive cells, and Sprr2a expression was increased along with the progress of taste bud development. These results demonstrate that regional gene expression surrounding taste buds is associated with taste bud formation and controlled by the innervating taste nerve. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

75 FR 51169 - OTS Minority Depository Institutions Advisory Committee

Science.gov (United States)

2010-08-18

... DEPARTMENT OF THE TREASURY Office of Thrift Supervision [Docket ID: OTS-2010-0026] OTS Minority... (OTS). ACTION: Notice. SUMMARY: The Charter for the OTS Minority Depository Institutions Advisory... of the Treasury to announce the renewal of the OTS Minority Depository Institutions Advisory...

CRISPR/Cas9 DNA cleavage at SNP-derived PAM enables both in vitro and in vivo KRT12 mutation-specific targeting.

Science.gov (United States)

Courtney, D G; Moore, J E; Atkinson, S D; Maurizi, E; Allen, E H A; Pedrioli, D M L; McLean, W H I; Nesbit, M A; Moore, C B T

2016-01-01

CRISPR/Cas9-based therapeutics hold the possibility for permanent treatment of genetic disease. The potency and specificity of this system has been used to target dominantly inherited conditions caused by heterozygous missense mutations through inclusion of the mutated base in the short-guide RNA (sgRNA) sequence. This research evaluates a novel approach for targeting heterozygous single-nucleotide polymorphisms (SNPs) using CRISPR/Cas9. We determined that a mutation within KRT12, which causes Meesmann's epithelial corneal dystrophy (MECD), leads to the occurrence of a novel protospacer adjacent motif (PAM). We designed an sgRNA complementary to the sequence adjacent to this SNP-derived PAM and evaluated its potency and allele specificity both in vitro and in vivo. This sgRNA was found to be highly effective at reducing the expression of mutant KRT12 mRNA and protein in vitro. To assess its activity in vivo we injected a combined Cas9/sgRNA expression construct into the corneal stroma of a humanized MECD mouse model. Sequence analysis of corneal genomic DNA revealed non-homologous end-joining repair resulting in frame-shifting deletions within the mutant KRT12 allele. This study is the first to demonstrate in vivo gene editing of a heterozygous disease-causing SNP that results in a novel PAM, further highlighting the potential for CRISPR/Cas9-based therapeutics.

The First Report of KRT5 Mutation Underlying Acantholytic Dowling-Degos Disease with Mottled Hypopigmentation in an Indian Family

Science.gov (United States)

Verma, Shyam; Pasternack, Sandra M.; Rütten, Arno; Ruzicka, Thomas; Betz, Regina C.; Hanneken, Sandra

2014-01-01

Galli Galli disease (GGD) is the name given to a rare form of acantholytic Dowling-Degos disease. (DDD), the latter itself being a rare condition. We believe we are describing for the first time in Indian dermatologic literature a case of GGD in a family where 25 persons have DDD and have been able to document a KRT5 mutation in four members of the family. Whereas reticulate pigmentation is a hallmark of DDD there are rare reports of mottled pigmentation with multiple asymptomatic hypopigmented macules scattered diffusely along with the pigmentation. All the cases described here show a mottled pigmentation comprising hypo and hyperpigmented asymptomatic macules. After the clinical diagnosis was made by one of the authors (SV) in India, the German authors repeated histological examination and successfully demonstrated a heterozygous nonsense mutation, c.C10T (p.Gln4X), in exon 1 of the KRT5 gene, from various centers in Munich, Bonn, Dusseldorf and Friedrichschafen in Germany. PMID:25284854

A categorization of customer concerns for an OT front-end of innovation process in an IT/OT convergence context

OpenAIRE

Bonnetto , Emilie; YANNOU , Bernard; Bertoluci , Gwenola; Boly , Vincent; Alvarez , Jorge

2016-01-01

International audience; Operational Technologies (OT) are designed to monitor and control plants. OT are increasingly mixed with Information Technologies (IT) in global solutions. A conventional customer inquiry is no more sufficient to get enough data about Customer Concerns (CC). Indeed, an IT OT solution is the nervous system of a company; it intertwines people processes and functions. For specification step, one must now capture negative perceptions in the interrelationships with other ac...

Fabrication of PEDOT-OTS-patterned ITO substrates

NARCIS (Netherlands)

Herzer, N.; Wienk, M.M.; Schmit, P.; Spoelstra, A.B.; Hendriks, C.E.; Oosterhout, S.D.; Höppener, S.; Schubert, U.S.

2010-01-01

The fabrication of a poly(3,4-ethylenedioxythiophene) (PEDOT) pattern is demonstrated. As template, an n-octadecyltrichlorosilane (OTS) monolayer self-assembled on indium tin oxide (ITO) was structured by UV–ozone photolithography, resulting in an ITO–OTS patterned surface. The conducting properties

Os instrumentos de regulação baseados no conhecimento: o caso do programme for international student assessment (PISA Knowledge-based regulation tools (KRT: the programme for international student assessment (PISA case

Directory of Open Access Journals (Sweden)

Estela Costa

2009-12-01

Full Text Available Este artigo inscreve-se na temática dos novos modos de regulação da educação, em particular nos instrumentos de políticas públicas. No quadro da instrumentação da acção pública, a atenção recai nos instrumentos de regulação baseados no conhecimento (Knowledge-based Regulation Tools [KRT]. Na verdade, a economia mundial fundamenta-se, cada vez mais, no conhecimento, o qual emerge como instrumento de política, regulando os actores sociais e legitimando a decisão política. O Programme for International Student Assessment (PISA, da Organização para Cooperação e Desenvolvimento Económico (OCDE, foi o instrumento escolhido como passível de configurar um KRT. O texto baseiase em seis estudos de caso, assentes em entrevistas e análise documental. Intentando na circulação e no uso do PISA, na Bélgica, Escócia, França, Hungria, Portugal e Roménia, procuramos demonstrar que o PISA se afirma como um KRT, provando a relação circular entre conhecimento e política, dado que, como instrumento de política, produz conhecimento e, como instrumento científico, produz política.This text focuses on the study of a KRT (Knowledgebased Regulation Tool, within the wider framework of the new modes of regulation in education, and use of instruments in public action. The world economy is gradually more knowledge-based. Knowledge emerges as a tool for policy-making and for regulating social actors, and legitimating the political decision-making. We choose the OECD's Program for International Student Assessment (PISA as an example of a KRT. The article is based on six case-studies, with interviews and document analysis. Our goal was to study the circulation and use of PISA, in Belgium, Scotland, France, Hungary, Portugal and Romania, trying to show that PISA is indeed a KRT, thus proving the circular relationship between knowledge and policy, because as a policy instrument it produces knowledge, and as a scientific tool it produces policy.

The chicken frizzle feather is due to an α-keratin (KRT75 mutation that causes a defective rachis.

Directory of Open Access Journals (Sweden)

Chen Siang Ng

Full Text Available Feathers have complex forms and are an excellent model to study the development and evolution of morphologies. Existing chicken feather mutants are especially useful for identifying genetic determinants of feather formation. This study focused on the gene F, underlying the frizzle feather trait that has a characteristic curled feather rachis and barbs in domestic chickens. Our developmental biology studies identified defects in feather medulla formation, and physical studies revealed that the frizzle feather curls in a stepwise manner. The frizzle gene is transmitted in an autosomal incomplete dominant mode. A whole-genome linkage scan of five pedigrees with 2678 SNPs revealed association of the frizzle locus with a keratin gene-enriched region within the linkage group E22C19W28_E50C23. Sequence analyses of the keratin gene cluster identified a 69 bp in-frame deletion in a conserved region of KRT75, an α-keratin gene. Retroviral-mediated expression of the mutated F cDNA in the wild-type rectrix qualitatively changed the bending of the rachis with some features of frizzle feathers including irregular kinks, severe bending near their distal ends, and substantially higher variations among samples in comparison to normal feathers. These results confirmed KRT75 as the F gene. This study demonstrates the potential of our approach for identifying genetic determinants of feather forms.

The potential for oxytocin (OT) to prevent breast cancer: a hypothesis.

Science.gov (United States)

Murrell, T G

1995-08-01

This hypothesis proposes that carcinogens in the breast are generated by the action of superoxide free radicals released when acinal gland distension, under the influence of unopposed prolactin, causes microvessel ischaemia. Inadequate nipple care in the at-risk years leads to ductal obstruction preventing the elimination of carcinogens from the breast. The regular production of oxytocin (OT) from nipple stimulation would cause contraction of the myoepithelial cells, relieving acinal gland distension and aiding the active elimination of carcinogenic fluid from the breast. Mechanical breast pump stimulation causes an increase in plasma OT levels in the luteal but not in the follicular phase of the menstrual cycle. OT production upon nipple stimulation in the luteal phase of premenopausal, non-lactating women may be protective against the high rates of mitotic breast cell division noted at this time via the potential to block the effect of oestrogen. The epidemiology of breast cancer suggests that lengthy lactation time is beneficial. Sexual activity in nulliparous women also protects and OT levels have been shown to rise with orgasm in women and in men. OT systems in the brain are intricately linked to oestrogen and progesterone levels, and it is possible that these hormones may modify the OT secretory response both centrally and through an effect on the sensitivity of the breast. OT production with nipple care and in sex and lactation, and the reduction in cycling ovarian hormones that occurs with pregnancy, may all be important preventative factors in the development of breast cancer both pre- and post-menopausally.

12 CFR 563b.115 - How will OTS review my business plan?

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How will OTS review my business plan? 563b.115... business plan? (a) OTS will review your business plan to determine that it demonstrates a safe and sound... will be determinative. OTS will review every case on its merits. (b) You must file your business plan...

12 CFR 500.10 - The OTS or The Office.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 5 2010-01-01 2010-01-01 false The OTS or The Office. 500.10 Section 500.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY AGENCY ORGANIZATION AND FUNCTIONS General Organization § 500.10 The OTS or The Office. The Office of Thrift Supervision (referred to as “OTS” or “Office”) is an office of the...

Superhump Period of MASTER OT

Science.gov (United States)

Garnavich, Peter; McClelland, Colin

2013-02-01

We observed the optical transient MASTER OT J065608.28+744455.2 (ATEL #4783) with the Vatican Advanced Technology Telescope (VATT) and VATT4K CCD camera. V-band imaging began at 2013 Feb. 5.15 (UT) and continued for 3.3 hours with a time resolution of 22 seconds.

12 CFR 563.146 - Will the OTS permit my capital distribution?

Science.gov (United States)

2010-01-01

... under 12 U.S.C. 1831o(d)(1)(B). (b) Your proposed capital distribution raises safety or soundness... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Will the OTS permit my capital distribution... SAVINGS ASSOCIATIONS-OPERATIONS Capital Distributions § 563.146 Will the OTS permit my capital...

12 CFR 502.55 - Where can I find OTS's fee schedule?

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Where can I find OTS's fee schedule? 502.55 Section 502.55 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND... fees in a Thrift Bulletin. OTS will publish these fees at least 30 days before they are effective. ...

Optimization of OT-MACH Filter Generation for Target Recognition

Science.gov (United States)

Johnson, Oliver C.; Edens, Weston; Lu, Thomas T.; Chao, Tien-Hsin

2009-01-01

An automatic Optimum Trade-off Maximum Average Correlation Height (OT-MACH) filter generator for use in a gray-scale optical correlator (GOC) has been developed for improved target detection at JPL. While the OT-MACH filter has been shown to be an optimal filter for target detection, actually solving for the optimum is too computationally intensive for multiple targets. Instead, an adaptive step gradient descent method was tested to iteratively optimize the three OT-MACH parameters, alpha, beta, and gamma. The feedback for the gradient descent method was a composite of the performance measures, correlation peak height and peak to side lobe ratio. The automated method generated and tested multiple filters in order to approach the optimal filter quicker and more reliably than the current manual method. Initial usage and testing has shown preliminary success at finding an approximation of the optimal filter, in terms of alpha, beta, gamma values. This corresponded to a substantial improvement in detection performance where the true positive rate increased for the same average false positives per image.

Performance of non-ideal OT-MRC with co-channel interference

KAUST Repository

Radaydeh, Redha Mahmoud Mesleh

2010-12-01

This paper studies the effect of non-ideal estimation of channel state information (CSI) on the performance of output-threshold maximal-ratio combining (OT-MRC) diversity scheme in the presence of co-channel interference as well as white noise. The channel fading envelopes are assumed to follow slowly varying flat Rayleigh model. New closed-form expressions for the combined signal-to-interference-plus-noise ratio (SINR) distribution and outage probability performance are presented. Performance comparisons between the conventional MRC and OT-MRC for the system model described above are provided. © 2010 IEEE.

PREDICTING PROGNOSTIC VALUE OF OCULAR TRAUMA SCORE (OTS IN AN OPEN GLOBE INJURY IN TERTIARY EYE CARE HOSPITAL

Directory of Open Access Journals (Sweden)

Rahul

2015-10-01

Full Text Available AIM : To evaluate the prognostic value of OTS in open globe injuries. MATERIAL METHOD : Retrospective analysis of 77 eyes with open globe injuries was done from 01/07/2013 to 31/12/2014. Patients were assigned raw score sum based on initial V/A, and ocular findings then classified into 5 categories for predicting final visual outcome based on ocular Trauma score (OTS. RESULT : We estimated final V/A in 77 cases of open globe injuries (64.93% had raw sc ore between 65.91 (category 3, 4 Six months after the injury, 42.85% patients of categories 1 (raw score 0 - 44 achieved V/A of PL/HM as compared to 17% in OTS study. 16 patients with raw compared to OTS study. We reported comparable visual outcome with OT S study except in category 1 & 2. CONCLUSION: OTS score is valuable in triage, patient counseling and decision making for the management of ocular trauma. We recommend that OTS should be used routinely for open globe injuries as it is a simple guide

12 CFR 516.10 - How does OTS compute time periods under this part?

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How does OTS compute time periods under this part? 516.10 Section 516.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES § 516.10 How does OTS compute time periods under this part? In computing...

12 CFR 516.170 - When will OTS conduct a meeting on an application?

Science.gov (United States)

2010-01-01

... application, or otherwise determines that a meeting will benefit the decision-making process. OTS may limit... inform the applicant and all commenters requesting a meeting of its decision to grant or deny a meeting request, or of its decision to conduct a meeting on its own initiative. (c) If OTS decides to conduct a...

Use of thermal cycling to reduce adhesion of OTS coated coated MEMS cantilevers

Science.gov (United States)

Ali, Shaikh M.; Phinney, Leslie M.

2003-01-01

°Microelectromechanical systems (MEMS) have enormous potential to contribute in diverse fields such as automotive, health care, aerospace, consumer products, and biotechnology, but successful commercial applications of MEMS are still small in number. Reliability of MEMS is a major impediment to the commercialization of laboratory prototypes. Due to the multitude of MEMS applications and the numerous processing and packaging steps, MEMS are exposed to a variety of environmental conditions, making the prediction of operational reliability difficult. In this paper, we investigate the effects of operating temperature on the in-use adhesive failure of electrostatically actuated MEMS microcantilevers coated with octadecyltrichlorosilane (OTS) films. The cantilevers are subjected to repeated temperature cycles and electrostatically actuated at temperatures between 25°C and 300°C in ambient air. The experimental results indicate that temperature cycling of the OTS coated cantilevers in air reduces the sticking probability of the microcantilevers. The sticking probability of OTS coated cantilevers was highest during heating, which decreased during cooling, and was lowest during reheating. Modifications to the OTS release method to increase its yield are also discussed.

"Merging Yoga and Occupational Therapy (MY-OT): A feasibility and pilot study".

Science.gov (United States)

Schmid, Arlene A; Puymbroeck, Marieke Van; Portz, Jennifer D; Atler, Karen E; Fruhauf, Christine A

2016-10-01

To examine the feasibility and benefits of the Merging Yoga and Occupational Therapy (MY-OT) intervention. This is the primary analysis of a non-controlled pretest-posttest pilot study to understand the feasibility and impact of MY-OT on balance, balance self-efficacy, and fall risk factor management in people with chronic stroke. University research laboratory. People with chronic stroke were included in the study if they: had sustained a fall or had fear of falling, were able to stand, and hand impaired balance and were at risk for falls (≤46 on the Berg Balance Scale (BBS)). Individuals completed an 8 week intervention that included 16 sessions of both yoga and group occupational therapy (OT). Yoga included physical postures, breathing exercises, and meditation. OT focused on post-stroke fall risk factor management. The BBS was used to assess balance, the Activities-specific Balance Confidence Scale (ABC) was used to measure balance self-efficacy. Five fall risk factor management scales were used. Overall, the intervention was considered feasible, as individuals were able to safely complete the intervention with little attrition and high attendance. Balance improved by 30% (p=0.002). Balance self-efficacy improved by 15% (p=0.034). Each of the five fall risk factor management scales improved, but only two significantly improved (Fall Prevention and Management Questionnaire, 29%, p=0.004 and Fall Prevention Strategy Survey, 42%, p=0.032). The results demonstrate that MY-OT is a potential intervention to improve multiple fall related outcomes for people with stroke. Therapists may consider these interventions for people with stroke, but additional research is warranted. Copyright © 2016 Elsevier Ltd. All rights reserved.

Trehalose-6-Phosphate-Mediated Toxicity Determines Essentiality of OtsB2 in Mycobacterium tuberculosis In Vitro and in Mice.

Directory of Open Access Journals (Sweden)

Jan Korte

2016-12-01

Full Text Available Trehalose biosynthesis is considered an attractive target for the development of antimicrobials against fungal, helminthic and bacterial pathogens including Mycobacterium tuberculosis. The most common biosynthetic route involves trehalose-6-phosphate (T6P synthase OtsA and T6P phosphatase OtsB that generate trehalose from ADP/UDP-glucose and glucose-6-phosphate. In order to assess the drug target potential of T6P phosphatase, we generated a conditional mutant of M. tuberculosis allowing the regulated gene silencing of the T6P phosphatase gene otsB2. We found that otsB2 is essential for growth of M. tuberculosis in vitro as well as for the acute infection phase in mice following aerosol infection. By contrast, otsB2 is not essential for the chronic infection phase in mice, highlighting the substantial remodelling of trehalose metabolism during infection by M. tuberculosis. Blocking OtsB2 resulted in the accumulation of its substrate T6P, which appears to be toxic, leading to the self-poisoning of cells. Accordingly, blocking T6P production in a ΔotsA mutant abrogated otsB2 essentiality. T6P accumulation elicited a global upregulation of more than 800 genes, which might result from an increase in RNA stability implied by the enhanced neutralization of toxins exhibiting ribonuclease activity. Surprisingly, overlap with the stress response caused by the accumulation of another toxic sugar phosphate molecule, maltose-1-phosphate, was minimal. A genome-wide screen for synthetic lethal interactions with otsA identified numerous genes, revealing additional potential drug targets synergistic with OtsB2 suitable for combination therapies that would minimize the emergence of resistance to OtsB2 inhibitors.

12 CFR 510.5 - Release of unpublished OTS information.

Science.gov (United States)

2010-01-01

... ORGANIZATIONAL REGULATIONS § 510.5 Release of unpublished OTS information. (a) Scope. (1) This section applies to... their official capacities. Examples of unpublished information include: (i) Information in the memory of...

Oxytocin (OT) and arginine-vasopressin (AVP) act on OT receptors and not AVP V1a receptors to enhance social recognition in adult Syrian hamsters (Mesocricetus auratus).

Science.gov (United States)

Song, Zhimin; Larkin, Tony E; Malley, Maureen O'; Albers, H Elliott

2016-05-01

Social recognition is a fundamental requirement for all forms of social relationships. A majority of studies investigating the neural mechanisms underlying social recognition in rodents have investigated relatively neutral social stimuli such as juveniles or ovariectomized females over short time intervals (e.g., 2h). The present study developed a new testing model to study social recognition among adult males using a potent social stimulus. Flank gland odors are used extensively in social communication in Syrian hamsters and convey important information such as dominance status. We found that the recognition of flank gland odors after a 3min exposure lasted for at least 24h, substantially longer than the recognition of other social cues in rats and mice. Intracerebroventricular injections of OT and AVP prolonged the recognition of flank gland odor for up to 48h. Selective OTR but not V1aR agonists, mimicked these enhancing effects of OT and AVP. Similarly, selective OTR but not V1aR antagonists blocked recognition of the odors after 20min. In contrast, the recognition of non-social stimuli was not blocked by either the OTR or the V1aR antagonists. Our findings suggest both OT and AVP enhance social recognition via acting on OTRs and not V1aRs and that the recognition enhancing effects of OT and AVP are limited to social stimuli. Copyright © 2016 Elsevier Inc. All rights reserved.

Recent optical activity of the blazar OT 355

Science.gov (United States)

Bachev, R.; Kurtenkov, A.; Nikolov, Y.; Spassov, B.; Boeva, S.; Latev, G.; Dimitrova, R. V. Munoz

2017-06-01

The Flat Spectrum Radio Quasar OT 355 (also known as 7C 173240.70+385949.00, z=0.975) was typically observed to be in the optical between 16th and 21th magnitude (CRTS, http://nesssi.cacr.caltech.edu/catalina/20011332/113321380764100137p.html).

A gene expression study of dorso-ventrally restricted pigment pattern in adult fins of Neolamprologus meeli, an African cichlid species

Directory of Open Access Journals (Sweden)

Ehsan Pashay Ahi

2017-01-01

Full Text Available Fish color patterns are among the most diverse phenotypic traits found in the animal kingdom. Understanding the molecular and cellular mechanisms that control in chromatophore distribution and pigmentation underlying this diversity is a major goal in developmental and evolutionary biology, which has predominantly been pursued in the zebrafish model system. Here, we apply results from zebrafish work to study a naturally occurring color pattern phenotype in the fins of an African cichlid species from Lake Tanganyika. The cichlid fish Neolamprologus meeli displays a distinct dorsal color pattern, with black and white stripes along the edges of the dorsal fin and of the dorsal half of the caudal fin, corresponding with differences in melanophore density. To elucidate the molecular mechanisms controlling the differences in dorsal and ventral color patterning in the fins, we quantitatively assessed the expression of 15 candidate target genes involved in adult zebrafish pigmentation and stripe formation. For reference gene validation, we screened the expression stability of seven widely expressed genes across the investigated tissue samples and identified tbp as appropriate reference. Relative expression levels of the candidate target genes were compared between the dorsal, striped fin regions and the corresponding uniform, grey-colored regions in the anal and ventral caudal fin. Dorso-ventral expression differences, with elevated levels in both white and black stripes, were observed in two genes, the melanosome protein coding gene pmel and in igsf11, which affects melanophore adhesion, migration and survival. Next, we predicted potential shared upstream regulators of pmel and igsf11. Testing the expression patterns of six predicted transcriptions factors revealed dorso-ventral expression difference of irf1 and significant, negative expression correlation of irf1 with both pmel and igsf11. Based on these results, we propose pmel, igsf11 and irf1 as

12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 5 2010-01-01 2010-01-01 false When will OTS adjust, add, waive, or eliminate a fee? 502.60 Section 502.60 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under...

Placental Expressions of CDKN1C and KCNQ1OT1 in Monozygotic Twins with Selective Intrauterine Growth Restriction.

Science.gov (United States)

Gou, Chenyu; Liu, Xiangzhen; Shi, Xiaomei; Chai, Hanjing; He, Zhi-Ming; Huang, Xuan; Fang, Qun

2017-10-01

CDKN1C and KCNQ1OT1 are imprinted genes that might be potential regulators of placental development. This study investigated placental expressions of CDKN1C and KCNQ1OT1 in monozygotic twins with and without selective intrauterine growth restriction (sIUGR). Seventeen sIUGR and fifteen normal monozygotic(MZ) twin pairs were examined. Placental mRNA expressions of CDKN1C and KCNQ1OT1 were detected by real-time fluorescent quantitative PCR. CDKN1C protein expression was detected by immunohistochemical assay and Western-blotting. In the sIUGR group, smaller fetuses had a smaller share of the placenta, and CDKN1C protein expression was significantly increased while KCNQ1OT1 mRNA expression was significantly decreased. The CDKN1C/KCNQ1OT1 mRNA ratio was lower in the larger fetus than in the smaller fetus (p < .05). In the control group, CDKN1C protein expression showed no difference between larger and smaller fetuses, while KCNQ1OT1 mRNA expression was significantly lower in the larger fetus, and the CDKN1C/KCNQ1OT1 mRNA ratio was higher in the larger fetus than in the smaller fetus (p < .05). Our findings showed that pathogenesis of sIUGR may be related to the co-effect of the up-regulated protein expression of CDKN1C and down-regulated mRNA expression of KCNQ1OT1 in the placenta.

OT - Education for the health services of the future

DEFF Research Database (Denmark)

Hansen, Bodil Winther; Sørensen, Annette; Hove, Anne

OT - Education for the health services of the future This presentation offers knowledge about which qualifications the health services and OT practice in general demand from Occupational Therapists. The study was developed in a wider context of the constant reflection within higher education...... as a result of the rapid pace of change in society. The Faculty of Occupational Therapy in Copenhagen wanted to enable the graduates in the best possible way to meet the employers’ demands as to qualifications. Furthermore, the aim was to develop and guarantee the quality of the educations offered...... by University College Oeresund. To express the level of education to be achieved in terms of competences and learning outcome, the study was inspired by the tuning process of educational structures in Europe, which is part of the Bologna process to integrate higher education area in Europe. The study is based...

Gene expression profiles of prohibitin in testes of Octopus tankahkeei (ot-phb) revealing its possible role during spermiogenesis.

Science.gov (United States)

Mao, Hai-Tao; Wang, Da-Hui; Lan, Zhou; Zhou, Hong; Yang, Wan-Xi

2012-05-01

Prohibitin is essential for intracellular homeostasis and stabilization of mitochondrial respiratory chain complexes. To explore its functions during spermiogenesis of Octopus tankahkeei (O. tankahkeei), we have cloned and sequenced the cDNA of this mammalian PHB homologue (termed ot-PHB) from the testes of O. tankahkeei. The 1165 bp ot-phb cDNA contains a 100 bp 5' UTR, a 882 bp open reading frame and a 183 bp 3' UTR. The putative ot-PHB protein owns a transmembrane domain from 6 to 31 amino acid (aa) and a putative PHB domain from 26 to 178 aa. Protein alignment demonstrated that ot-PHB had 73.3, 73.6, 74.0, 75.1, and 45.4% identity with its homologues in Homo sapiens, Mus muculus, Danio rerio, Xenopus tropicalis and Trypanosoma brucei, respectively. Tissue distribution profile analysis revealed its presence in all the tissues examined. In situ hybridization in spermiogenic cells demonstrated that ot-phb was expressed moderately at the beginning of the spermiogenesis. The abundance of transcripts increased in intermediate spermatids and in drastically remodeling final spermatids. In mature spermatozoa, the residuary transcripts concentrated around the chondriosomal mantle where mitochondria assemble around. In summary, the expression of ot-phb during spermiogenesis implicates a potential function of this protein during mitochondrial ubiquitination. It is the first time to implicate the role of prohibitin in cephalopod spermiogenesis.

Changeover Inference: Estimating the Relationship Between DT and OT Data

National Research Council Canada - National Science Library

Dippery, Kevin

1997-01-01

... which has undergone developmental testing. Using a re-sampling method called the Bootstrap, the sampling variance and standard error of the changeover factor are calculated, as are confidence intervals for the OT failure rate of a new system...

12 CFR 502.50 - What fees does OTS charge?

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 5 2010-01-01 2010-01-01 false What fees does OTS charge? 502.50 Section 502.50 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND FEES... consolidated with a savings association on the Consolidated Statement of Condition of the Thrift Financial...

Spectroscopic Classification of MASTER OT J044212.20+230616.7

Science.gov (United States)

Shivvers, I.; Kelly, P. L.; Clubb, K. I.; Filippenko, A. V.

2014-09-01

We report that inspection of a CCD spectrum (range 330-1000 nm), obtained on September 22 UT with the 3-m Shane reflector (+ Kast) at Lick Observatory, shows that MASTER OT J044212.20+230616.7 (ATel #6484) is a Type IIn supernova.

Doktoritööd : [2007, Janis Ešots, Signe Dobelniece

Index Scriptorium Estoniae

2007-01-01

29. mail kaitseb Janis Ešots doktoritööd "Mullā Sadrā wujūd õpetus: müstitsismi ja filosoofia süntees"; 11. juunil kaitseb Signe Dobelniece doktoritööd "Kodutus Lätis: põhjused ja lahendused"

The reactivity of anion-exchange resins by applying OT-for-OH exchange reaction in the equilibrium state

International Nuclear Information System (INIS)

Kano, Naoki; Nihei, Makoto; Imaizumi, Hiroshi

1996-01-01

In order to reveal the behavior of hydroxyl group in isotope exchange reaction, OT-for-OH exchange reaction between each anion-exchange resin (OH - form) and tritiated water (abbreviated as HTO water below) was observed at 80degC under the equilibrium. Anion-exchange resins used were Amberlite IRA-400, IRA-410 (both strongly basic), and IRA-94S (weakly basic). It can be thought that an HTO molecule dissociates into H + +OT - (or T + +OH - ). The activity of each resin based on OT-for-OH exchange reaction was measured with a liquid scintillation counter. From the above-mentioned, the following five were found. Isotope exchange reaction as 'atomic group' occurred between the OH group in each anion-exchange resin and the OT group in HTO water. The reactivity of strongly basic anion-exchange resin is larger than that of weakly basic one. The ratio of the reactivity of these resins can roughly be expressed as follows: (IRA-410): (IRA-400): (IRA-94S)=42: 7: 1. The degree of OT-for-OH exchange reaction may be smaller than that of T-for-H exchange reaction. The method used and results obtained in this work may be helpful to obtain the data for the prevention of T-contamination, especially to obtain the data from certain atomic groups including T. (author)

Over-expression of the mycobacterial trehalose-phosphate phosphatase OtsB2 results in a defect in macrophage phagocytosis associated with increased mycobacterial-macrophage adhesion

Directory of Open Access Journals (Sweden)

Hao Li

2016-11-01

Full Text Available Trehalose-6-phosphate phosphatase (OtsB2 is involved in the OtsAB trehalose synthesis pathway to produce free trehalose and is strictly essential for mycobacterial growth. We wished to determine the effects of OtsB2 expression on mycobacterial phenotypes such as growth, phagocytosis and survival in macrophages. Mycobacterium bovis-BCG (BCG over-expressing OtsB2 were able to better survive in stationary phase. Over-expression of OtsB2 led to a decrease in phagocytosis but not survival in THP-1 macrophage-like cells, and this was not due to a decrease in general macrophage phagocytic activity. Surprisingly, when we investigated macrophage-mycobacterial interactions by flow cytometry and atomic force microscopy, we discovered that BCG over-expressing OtsB2 have stronger binding to THP-1 cells than wild-type BCG. These results suggest that altering OtsB2 expression has implications for mycobacterial host-pathogen interactions. Macrophage-mycobacteria phagocytic interactions are complex and merit further study.

Inflatable capsule recovery raft using OTS pontoons and other components, Phase I

Data.gov (United States)

National Aeronautics and Space Administration — The objective of the proposed innovation is to research and study the feasibility of using pontoons (based on Off the Shelf (OTS) equipment) to make an inflatable...

MASTER OT J072007.30+451611.6: Cataclysmic Variable with an Extreme Hot Spot

Science.gov (United States)

Denisenko, D.

2018-05-01

MASTER team has reported an unusual object MASTER OT J072007.30+451611.6 in ATel #11620 (T. Pogrosheva et al.). The object was observed at 17.0-17.2m during 9 minutes from 22:15:26 to 22:24:30 UT on 2018-04-26, but was below the detection limit (18.8m) 45 minutes before the first positive observation and 43 minutes after the last one. As noted by S. Otero in AAVSO VSX entry, MASTER OT J072007.30+451611.6 has an X-ray counterpart XMMSL2 J072007.4+451615.

High-Amplitude, Rapid Photometric Variation of the New Polar Master OT J132104.0+560957.8

Science.gov (United States)

2015-02-05

HIGH-AMPLITUDE, RAPID PHOTOMETRIC VARIATION OF THE NEW POLAR MASTER OT J132104.04+560957.8 LITTLEFIELD, COLIN;1,2 GARNAVICH, PETER;1 MAGNO, KATRINA;1...18.5 during each photometric cycle, becoming so faint that we could no longer detect it. The data showed a period of roughly 91 minutes with each...TYPE 3. DATES COVERED 00-00-2015 to 00-00-2015 4. TITLE AND SUBTITLE High-Amplitude, Rapid Photometric Variation Of The New Polar Master OT

MASTER-SAAO: contradictory SN and flaring OT

Science.gov (United States)

Balanutsa, P.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Gress, O.; Pogrosheva, T.; Shumkov, V.; Vladimirov, V.; Vlasenko, D.; Kuvshinov, D.; Gabovich, A.

2017-07-01

MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 03h 42m 50.70s , -01d 52m 28s.7 on 2017-07-01.1753UT with unfiltered (6 images), that contradicts to Ia type detected in ATEL #10240 , ATEL #10225 for ATLAS17dcl ( http://www.supernova.thistlethwaites.com/sn2017/sndate.html ). It is in 8.4"W,20"S of PGC135685 with Btc=15.13, Vgsr=2773 http://leda.univ-lyon1.fr/ledacat.cgi?PGC135685 Spectral observations are required.

Solar energy system performance evaluation: Honeywell OTS 41, Shenandoah (Newman), Georgia

Science.gov (United States)

Mathur, A. K.; Pederson, S.

1982-08-01

The operation and technical performance of the Solar Operational Test Site (OTS 41) located at Shenandoah, Georgia, are described, based on the analysis of the data collected between January and August 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 41 is a hydronic heating and cooling system consisting of 702 square feet of liquid-cooled flat-plate collectors; a 1000-gallon thermal storage tank; a 3-ton capacity organic Rankine-cycle-engine-assisted air conditioner; a water-to-are heat exchanger for solar space heating; a finned-tube coil immersed in the storage tank to preheat water for a gas-fired hot water heater; and associated piping, pumps, valves, and controls. The solar system has six basic modes of operation and several combination modes. The system operation is controlled automatically by a Honeywell-designed microprocessor-based control system, which also provides diagnostics.

Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis

Directory of Open Access Journals (Sweden)

Carmel M. Barber

2016-02-01

Full Text Available Taipans (Oxyuranus spp. are elapids with highly potent venoms containing presynaptic (β and postsynaptic (α neurotoxins. O. temporalis (Western Desert taipan, a newly discovered member of this genus, has been shown to possess venom which displays marked in vitro neurotoxicity. No components have been isolated from this venom. We describe the characterization of α-elapitoxin-Ot1a (α-EPTX-Ot1a; 6712 Da, a short-chain postsynaptic neurotoxin, which accounts for approximately 30% of O. temporalis venom. α-Elapitoxin-Ot1a (0.1–1 µM produced concentration-dependent inhibition of indirect-twitches, and abolished contractile responses to exogenous acetylcholine and carbachol, in the chick biventer cervicis nerve-muscle preparation. The inhibition of indirect twitches by α-elapitoxin-Ot1a (1 µM was not reversed by washing the tissue. Prior addition of taipan antivenom (10 U/mL delayed the neurotoxic effects of α-elapitoxin-Ot1a (1 µM and markedly attenuated the neurotoxic effects of α-elapitoxin-Ot1a (0.1 µM. α-Elapitoxin-Ot1a displayed pseudo-irreversible antagonism of concentration-response curves to carbachol with a pA2 value of 8.02 ± 0.05. De novo sequencing revealed the main sequence of the short-chain postsynaptic neurotoxin (i.e., α-elapitoxin-Ot1a as well as three other isoforms found in O. temporalis venom. α-Elapitoxin-Ot1a shows high sequence similarity (i.e., >87% with other taipan short-chain postsynaptic neurotoxins.

Nedožité devadesátiny Milana Otáhala

Czech Academy of Sciences Publication Activity Database

Tůma, Oldřich

2017-01-01

Roč. 24, 1/2 (2017), s. 247-251 ISSN 1210-7050 Institutional support: RVO:68378114 Keywords : Milan Otáhal * obituary * historiography Subject RIV: AB - History OBOR OECD: History (history of science and technology to be 6.3, history of specific sciences to be under the respective headings)

12 CFR 502.28 - How does OTS determine the organizational form component for a savings and loan holding company?

Science.gov (United States)

2010-01-01

... component for a savings and loan holding company? 502.28 Section 502.28 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND FEES Assessments Savings and Loan Holding Companies... savings and loan holding company that OTS regulates under section 10(l) of the HOLA. OTS will compute your...

Solar-energy-system performance evaluation: Honeywell OTS 44, Ocmulgee, Georgia

Science.gov (United States)

Mathur, A. K.; Pederson, S.

1982-01-01

The operation and technical performance of the solar operational test site (OTS 44) are described, based on data collected between April, 1981 and August, 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 44 is a hydronic heating and cooling system consisting of 5040 square feet of liquid cooled flat plate collectors; a 4000 gallon thermal storage tank; one 25 ton capacity organic Rankine cycle engine assisted water chillers; a forced draft cooling tower; and associated piping, pumps, valves, controls and heat rejection equipment. The solar system has eight basic modes of operation and several combination modes for providing space conditioning and hot water to the building. Data monitored during the 4 months of the operational test period found that the solar system collected 285 MMBtu of thermal energy of the total incident solar energy of 1040 MMBtu and provided 210 MMBtu for cooling and 10 MMBtu for heating and hot water. The net electrical energy saving due to the solar system was approximately 2600 kWh(e), and fossil energy saving was about 20 million Btu (MMBtu).

In a changing society which elements and competencies is needed and demanded in the future OT education?

DEFF Research Database (Denmark)

Hansen, Bodil Winther; Sørensen, Annette

to plan a curriculum, in order to enable OT graduates to meet employers’ demands in a rapidly changing society •The future OT profession. After 12 years of practising Problem Based Learning (PBL), the Faculty of Occupational Therapy wanted to develop the quality of the OT education offered by Metropolitan...... University College, Copenhagen. The object was to evaluate and develop the pedagogical practice, and to examine if the pedagogical concept prepares the students for the future in the best way possible. To describe the level of education to be achieved in terms of competences and learning outcome, the study...... was inspired by the Tuning Process of educational structures in Europe, which is part of the Bologna process to integrate higher education in Europe. The study is based on literature studies, quantitative and qualitative methods. All of these methods are suitable for uncovering competences demanded by practice...

Sequence analysis and heterologous expression of the wool cuticle-degrading enzyme encoding genes in Fusarium oxysporum 26-1.

Science.gov (United States)

Chaya, Etsushi; Suzuki, Tohru; Karita, Shuichi; Hanya, Akira; Yoshino-Yasuda, Shoko; Kitamoto, Noriyuki

2014-06-01

Two protease-like proteins, KrtA and KrtC, were identified in Fusarium oxysporum 26-1. Genes coding these proteins, krtA and krtC, were isolated and characterized. Recombinant KrtA (rKrtA) and KrtC (rKrtC) were successfully expressed in Aspergillus oryzae and secreted. The combination of rKrtA and rKrtC completely removed the cuticle of wool fibers. Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Optimal Diphthongs: An OT Analysis of the Acquisition of Spanish Diphthongs

Science.gov (United States)

Krause, Alice

2013-01-01

This dissertation investigates the acquisition of Spanish diphthongs by adult native speakers of English. The following research questions will be addressed: 1) How do adult native speakers of English pronounce sequences of two vowels in their L2 Spanish at different levels of acquisition? 2) Can OT learnability models, specifically the GLA,…

Viis korrust klaasi ja betooni / Ell-Maaja Randküla

Index Scriptorium Estoniae

Randküla, Ell-Maaja, 1939-2016

2005-01-01

Hotell Ülemiste Tallinnas Lennujaama tee 2. Sisekujundus: Meelis Press ja Hillar Mänd, AS Meelis Pressi Arhitektuuribüroo. Ehitaja: AS FKSM. Arhitekt Martin Aunin. Meelis Pressist ja Hillar Männist, nende tähtsamad tööd. Ill.: 21 värv. vaadet

LATERAL SURVIVAL: AN OT ACCOUNT

Directory of Open Access Journals (Sweden)

Moira Yip

2004-12-01

Full Text Available When laterals are the targets of phonological processes, laterality may or may not survive. In a fixed feature geometry, [lateral] should be lost if its superordinate node is eliminated by either the spreading of a neighbouring node, or by coda neutralization. So if [lateral] is under Coronal (Blevins 1994, it should be lost under Place assimilation, and if [lateral] is under Sonorant Voicing (Rice & Avery 1991 it should be lost by rules that spread voicing. Yet in some languages lateral survives such spreading intact. Facts like these argue against a universal attachment of [lateral] under either Coronal or Sonorant Voicing, and in favour of an account in terms of markedness constraints on feature-co-occurrence (Padgett 2000. The core of an OT account is that IFIDENTLAT is ranked above whatever causes neutralization, such as SHARE-F or *CODAF. laterality will survive. If these rankings are reversed, we derive languages in which laterality is lost. The other significant factor is markedness. High-ranked feature co-occurrence constraints like *LATDORSAL can block spreading from affecting laterals at all.

12 CFR 563b.200 - What actions may OTS take on my application?

Science.gov (United States)

2010-01-01

... appraiser may serve as an underwriter or selling agent, if you ensure that the appraiser is separate from the underwriter or selling agent affiliate and the underwriter or selling agent affiliate does not... convenience and needs of your communities after the conversion. (1) Based on this review, OTS may approve your...

The R package otu2ot for implementing the entropy decomposition of nucleotide variation in sequence data

Directory of Open Access Journals (Sweden)

Alban eRamette

2014-11-01

Full Text Available Oligotyping is a novel, supervised computational method that classifies closely related sequences into oligotypes (OTs based on subtle nucleotide variations (Eren et al. 2013. Its application to microbial datasets has helped reveal ecological patterns which are often hidden by the way sequence data are currently clustered to define operational taxonomic units (OTUs. Here, we implemented the OT entropy decomposition procedure and its unsupervised version, Minimal Entropy Decomposition (MED; Eren et al. 2014, in the statistical programming language and environment, R. The aims are to facilitate the integration of computational routines, interactive statistical analyses, and visualization into a single framework. In addition, two complementary approaches are implemented: 1 An analytical method (the broken stick model is proposed to help identify oligotypes of low abundance that could be generated by chance alone and 2 a one-pass profiling (OP method, to efficiently identify those OTUs whose subsequent oligotyping would be most promising. These enhancements are especially useful for large datasets, where a manual screening of entropy analysis results and the creation of a full set of OTs may not be feasible. The package and procedures are illustrated by several tutorials and examples.

Treatment of geographic atrophy by the topical administration of OT-551: results of a phase II clinical trial.

Science.gov (United States)

Wong, Wai T; Kam, Waynekid; Cunningham, Denise; Harrington, Molly; Hammel, Keri; Meyerle, Catherine B; Cukras, Catherine; Chew, Emily Y; Sadda, Srinivas R; Ferris, Frederick L

2010-12-01

To investigate the safety and preliminary efficacy of OT-551, a disubstituted hydroxylamine with antioxidant properties, for the treatment of geographic atrophy (GA), the advanced atrophic form of age-related macular degeneration (AMD). The study was a single-center, open-label phase II trial, enrolling 10 participants with bilateral GA. Topical 0.45% OT-551 was administered in one randomly assigned eye three times daily for 2 years. Safety measures were assessed by complete ophthalmic examination, fundus photography, and review of symptoms. The primary efficacy outcome measure was the change in best corrected visual acuity at 24 months. Secondary efficacy measures included changes in area of GA, contrast sensitivity, microperimetry measurements, and total drusen area from baseline. Study drug was well tolerated and was associated with few adverse events. The mean change in BCVA at 2 years was +0.2 ± 13.3 letters in the study eyes and -11.3 ± 7.6 letters in fellow eyes (P = 0.0259). However, no statistically significant differences were found between the study and fellow eyes for all other secondary outcome measures. OT-551 was well tolerated by study participants and was not associated with any serious adverse effects. Efficacy measurements in this small study indicate a possible effect in maintaining visual acuity. However, the absence of significant effects on other outcomes measures in this study suggests that OT-551, in the current concentration and mode of delivery, may have limited or no benefit as a treatment for GA (ClinicalTrials.gov number, NCT00306488).

78 FR 76768 - Removal of Transferred OTS Regulations Regarding Disclosure and Reporting of CRA-Related...

Science.gov (United States)

2013-12-19

... of competent jurisdiction, or by operation of law. \\1\\ Dodd-Frank Wall Street Reform and Consumer... the OTS was abolished by Title III of the Dodd-Frank Act. Part 390, subpart H is largely redundant of...

Evaluation and selection of CASE tool for SMART OTS development

International Nuclear Information System (INIS)

Park, K. O; Seo, S. M.; Seo, Y. S.; Koo, I. S.; Jang, M. H.

1999-01-01

CASE(Computer-Aided Software Engineering) tool is a software that aids in software engineering activities such as requirement analysis, design, testing, configuration management, and project management. The evaluation and selection of commercial CASE tools for the specific software development project is not a easy work because the technical ability of an evaluator and the maturity of a software development organization are required. In this paper, we discuss selection strategies, characteristic survey, evaluation criteria, and the result of CASE tool selection for the development of SMART(System-integrated Modular Advanced ReacTor) OTS(Operator Training Simulator)

VizieR Online Data Catalog: Follow-up photometry of M101 OT2015-1 (Blagorodnova+, 2017)

Science.gov (United States)

Blagorodnova, N.; Kotak, R.; Polshaw, J.; Kasliwal, M. M.; Cao, Y.; Cody, A. M.; Doran, G. B.; Elias-Rosa, N.; Fraser, M.; Fremling, C.; Gonzalez-Fernandez, C.; Harmanen, J.; Jencson, J.; Kankare, E.; Kudritzki, R.-P.; Kulkarni, S. R.; Magnier, E.; Manulis, I.; Masci, F. J.; Mattila, S.; Nugent, P.; Ochner, P.; Pastorello, A.; Reynolds, T.; Smith, K.; Sollerman, J.; Taddia, F.; Terreran, G.; Tomasella, L.; Turatto, M.; Vreeswijk, P. M.; Wozniak, P.; Zaggia, S.

2017-07-01

The location of M101-OT2015-1 has been serendipitously imaged by numerous telescopes and instruments over the last 15 years (from 2000 to 2015). Our best quality pre-discovery image (seeing of 0.55") is an r-band exposure at -3625 days pre-peak from the Canada-France-Hawaii Telescope (CFHT). The historical optical data for M101-OT was retrieved from the CFHT MegaPrime and CFHT12K/Mosaic, using single and combined exposures, Pan-STARRS-1/GPC1 (PS1), Isaac Newton Telescope/Wide Field Camera (INT/WFC), and Sloan Digital Sky Survey (SDSS) DR 10 (Ahn+ 2014, see V/147). Unfortunately, there are no HST images covering the location of the source. Post-discovery optical magnitudes were obtained from the reported followup astronomer's telegrams (ATels), Liverpool Telescope (LT), the Nordic Optical Telescope (NOT), and the Palomar P48 and P60 telescopes. The infrared data were retrieved from CFHT/WIRCam, UKIRT/WFCAM, and the Spitzer Infrared Array Camera in 3.6 and 4.5um as part of the SPitzer InfraRed Intensive Transients Survey (SPIRITS) (Kasliwal+, 2017ApJ...839...88K). Details of pre-discovery photometry and post-discovery optical photometry may be found in the Appendices Tables 1 and 2, respectively. We obtained spectra of M101-OT using a range of facilities in 2015 Feb-Jul. (3 data files).

Mis mõtteid tekitab õpetaja kutsestandardi projekt? / Kerli Prass, Kalle Kalda, Loone Ots, Külli Arand

Index Scriptorium Estoniae

2013-01-01

Vastasid SA Noored Kooli tuutor ja õpetajakoolituse koordinaator Kerli Prass, lastekaitse liidu president Loone Ots, Tartu haridustöötajate liidu esimees Kalle Kalda, Tallinna Saksa Gümnaasiumi klassiõpetaja Külli Arand

Keratin 17 Is Induced in Oral Cancer and Facilitates Tumor Growth.

Directory of Open Access Journals (Sweden)

Rumana Khanom

Full Text Available Keratin subtypes are selectively expressed depending on the cell type. They not only provide structural support, but regulate the metabolic processes and signaling pathways that control the growth of the epithelium. KRT17 (keratin 17 is induced in the regenerative epithelium and acts on diverse signaling pathways. Here, we demonstrate that KRT17 is invariably and permanently induced in oral squamous cell carcinoma (OSCC, as revealed by immunohistochemistry and cDNA microarray analysis. Two representative OSCC cell lines; KRT17-weakly expressing Ca9-22 and KRT17-highly expressing HSC3 were used to establish KRT17-overexpressing Ca9-22 and KRT17-knockdown HSC3 cells. Analysis of these cells revealed that KRT17 promoted cell proliferation and migration by stimulating the Akt/mTOR pathway. KRT17 also upregulated the expression of SLC2A1 (solute carrier family 2 member 1/Glut1 and glucose uptake. To further investigate the effect of KRT17 on tumorigenesis, KRT17-knockout HSC3 cells were established and were transplanted to the cephalic skin of nude mice. The tumors that developed from KRT17-knockout HSC3 cells had a lower Ki-67 labeling index and were significantly smaller compared to the controls. These results indicate that KRT17 stimulates the Akt/mTOR pathway and glucose uptake, thereby facilitating tumor growth. We could not confirm the relationship between KRT17 and SFN (stratifin in the cells examined in this study. However, our study reinforces the concept that the cellular properties of cancer are regulated by a series of molecules similar to those found in wound healing. In OSCC, KRT17 acts as a pathogenic keratin that facilitates tumor growth through the stimulation of multiple signaling pathways, highlighting the importance of KRT17 as a multifunctional promoter of tumorigenesis.

75 FR 181 - Application for Conversion From: (a) OTS-Regulated, State-Chartered Savings Association to...

Science.gov (United States)

2010-01-04

... DEPARTMENT OF THE TREASURY Office of Thrift Supervision Application for Conversion From: (a) OTS-Regulated, State- Chartered Savings Association to Federal Savings Association; (b) National Bank, Commercial Bank, State Savings Bank, or Credit Union to Federal Savings Association; (c) State Mutual Holding...

75 FR 10560 - Application for Conversion From: (a) OTS-Regulated, State-Chartered Savings Association to...

Science.gov (United States)

2010-03-08

... DEPARTMENT OF THE TREASURY Office of Thrift Supervision Application for Conversion From: (a) OTS-Regulated, State- Chartered Savings Association to Federal Savings Association; (b) National Bank, Commercial Bank, State Savings Bank, or Credit Union to Federal Savings Association; (c) State Mutual Holding...

Genetic background effects of keratin 8 and 18 in a DDC-induced hepatotoxicity and Mallory-Denk body formation mouse model.

Science.gov (United States)

Haybaeck, Johannes; Stumptner, Cornelia; Thueringer, Andrea; Kolbe, Thomas; Magin, Thomas M; Hesse, Michael; Fickert, Peter; Tsybrovskyy, Oleksiy; Müller, Heimo; Trauner, Michael; Zatloukal, Kurt; Denk, Helmut

2012-06-01

Keratin 8 (K8) and keratin 18 (K18) form the major hepatocyte cytoskeleton. We investigated the impact of genetic loss of either K8 or K18 on liver homeostasis under toxic stress with the hypothesis that K8 and K18 exert different functions. krt8⁻/⁻ and krt18⁻/⁻ mice crossed into the same 129-ola genetic background were treated by acute and chronic administration of 3,5-diethoxy-carbonyl-1,4-dihydrocollidine (DDC). In acutely DDC-intoxicated mice, macrovesicular steatosis was more pronounced in krt8⁻/⁻ and krt18⁻/⁻ compared with wild-type (wt) animals. Mallory-Denk bodies (MDBs) appeared in krt18⁻/⁻ mice already at an early stage of intoxication in contrast to krt8⁻/⁻ mice that did not display MDB formation when fed with DDC. Keratin-deficient mice displayed significantly lower numbers of apoptotic hepatocytes than wt animals. krt8⁻/⁻, krt18⁻/⁻ and control mice displayed comparable cell proliferation rates. Chronically DDC-intoxicated krt18⁻/⁻ and wt mice showed a similarly increased degree of steatohepatitis with hepatocyte ballooning and MDB formation. In krt8⁻/⁻ mice, steatosis was less, ballooning, and MDBs were absent. krt18⁻/⁻ mice developed MDBs whereas krt8⁻/⁻ mice on the same genetic background did not, highlighting the significance of different structural properties of keratins. They are independent of the genetic background as an intrinsic factor. By contrast, toxicity effects may depend on the genetic background. krt8⁻/⁻ and krt18⁻/⁻ mice on the same genetic background show similar sensitivity to DDC intoxication and almost resemble wt animals regarding survival, degree of porphyria, liver-to-body weight ratio, serum bilirubin and liver enzyme levels. This stands in contrast to previous work where krt8⁻/⁻ and krt18⁻/⁻ mice on different genetic backgrounds were investigated.

{sigma}{sup t}ot{sub e}eyy at e{sup +}e{sup -} colliders

Energy Technology Data Exchange (ETDEWEB)

Godbole, R.M. [Indian Institute of Science, Centre for Theoretical Studies, Bangalore (India); Pancheri, G. [Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali di Frascati, Rome (Italy)

2001-02-01

In this talk are briefly summarized different models for {sigma}{sup t}ot{sub 2}y (e{sup +}e{sup -} {yields}yy{yields} hadrons) and contrast model predictions with the data. It will be then discussed the capability of the future e{sup +}e{sup -} and yy colliders to distinguish between various models and end with an outlook for future work.

Návrh systému regulace vrtule stálých otáček

OpenAIRE

Líška, Lukáš

2014-01-01

Diplomová práca sa zaoberá problematikou návrhu elektrickej regulácie vrtule konštantných otáčok. Cieľom práce je ukázať postup návrhu regulátora na príklade vrtule DMP-3 pracujúcej s motorom Rotax 912 ULS, využitím vedomostí z mechaniky letu, elektrotechniky a automatizácie. V rámci práce bude poukázané na problematiku riadenia uhla nastavenia vrtule za použitia elektricky riadeného lineárneho aktuátora. Záver práce tvorí porovnanie výhod vrtule konštantných otáčok DMP-3 uvážením jej použiti...

Transcriptional role of androgen receptor in the expression of long non-coding RNA Sox2OT in neurogenesis.

Directory of Open Access Journals (Sweden)

Valentina Tosetti

Full Text Available The complex architecture of adult brain derives from tightly regulated migration and differentiation of precursor cells generated during embryonic neurogenesis. Changes at transcriptional level of genes that regulate migration and differentiation may lead to neurodevelopmental disorders. Androgen receptor (AR is a transcription factor that is already expressed during early embryonic days. However, AR role in the regulation of gene expression at early embryonic stage is yet to be determinate. Long non-coding RNA (lncRNA Sox2 overlapping transcript (Sox2OT plays a crucial role in gene expression control during development but its transcriptional regulation is still to be clearly defined. Here, using Bicalutamide in order to pharmacologically inactivated AR, we investigated whether AR participates in the regulation of the transcription of the lncRNASox2OTat early embryonic stage. We identified a new DNA binding region upstream of Sox2 locus containing three androgen response elements (ARE, and found that AR binds such a sequence in embryonic neural stem cells and in mouse embryonic brain. Our data suggest that through this binding, AR can promote the RNA polymerase II dependent transcription of Sox2OT. Our findings also suggest that AR participates in embryonic neurogenesis through transcriptional control of the long non-coding RNA Sox2OT.

Downregulation of keratin 76 expression during oral carcinogenesis of human, hamster and mouse.

Directory of Open Access Journals (Sweden)

Srikant Ambatipudi

Full Text Available Keratins are structural marker proteins with tissue specific expression; however, recent reports indicate their involvement in cancer progression. Previous study from our lab revealed deregulation of many genes related to structural molecular integrity including KRT76. Here we evaluate the role of KRT76 downregulation in oral precancer and cancer development.We evaluated KRT76 expression by qRT-PCR in normal and tumor tissues of the oral cavity. We also analyzed K76 expression by immunohistochemistry in normal, oral precancerous lesion (OPL, oral squamous cell carcinoma (OSCC and in hamster model of oral carcinogenesis. Further, functional implication of KRT76 loss was confirmed using KRT76-knockout (KO mice.We observed a strong association of reduced K76 expression with increased risk of OPL and OSCC development. The buccal epithelium of DMBA treated hamsters showed a similar trend. Oral cavity of KRT76-KO mice showed preneoplastic changes in the gingivobuccal epithelium while no pathological changes were observed in KRT76 negative tissues such as tongue.The present study demonstrates loss of KRT76 in oral carcinogenesis. The KRT76-KO mice data underlines the potential of KRT76 being an early event although this loss is not sufficient to drive the development of oral cancers. Thus, future studies to investigate the contributing role of KRT76 in light of other tumor driving events are warranted.

Genetic variants in pachyonychia congenita-associated keratins increase susceptibility to tooth decay.

Science.gov (United States)

Duverger, Olivier; Carlson, Jenna C; Karacz, Chelsea M; Schwartz, Mary E; Cross, Michael A; Marazita, Mary L; Shaffer, John R; Morasso, Maria I

2018-01-01

Pachyonychia congenita (PC) is a cutaneous disorder primarily characterized by nail dystrophy and painful palmoplantar keratoderma. PC is caused by mutations in KRT6A, KRT6B, KRT6C, KRT16, and KRT17, a set of keratin genes expressed in the nail bed, palmoplantar epidermis, oral mucosal epithelium, hair follicle and sweat gland. RNA-seq analysis revealed that all PC-associated keratins (except for Krt6c that does exist in the mouse genome) are expressed in the mouse enamel organ. We further demonstrated that these keratins are produced by ameloblasts and are incorporated into mature human enamel. Using genetic and intraoral examination data from 573 adults and 449 children, we identified several missense polymorphisms in KRT6A, KRT6B and KRT6C that lead to a higher risk for dental caries. Structural analysis of teeth from a PC patient carrying a p.Asn171Lys substitution in keratin-6a (K6a) revealed disruption of enamel rod sheaths resulting in altered rod shape and distribution. Finally, this PC-associated substitution as well as more frequent caries-associated SNPs, found in two of the KRT6 genes, that result in p.Ser143Asn substitution (rs28538343 in KRT6B and rs151117600 in KRT6C), alter the assembly of K6 filaments in ameloblast-like cells. These results identify a new set of keratins involved in tooth enamel formation, distinguish novel susceptibility loci for tooth decay and reveal additional clinical features of pachyonychia congenita.

Wannabe - Ameerika ja Postkontor 2 / Karin Paulus

Index Scriptorium Estoniae

Paulus, Karin, 1975-

2008-01-01

Uued hotellid Tallinna kesklinnas: Kaksiktornid (arhitektuuribüroo Nord Projekt, Meeli Truu, sisekujundaja Meelis Press); Forumi hotell (arhitektuuribüroo Pluss, Indrek Allmann, sisekujundajad Kaire Kemp-Tishler ja Ea Andla)

Atonen plaanib praamiliinid riigile anda / Hindrek Riikoja

Index Scriptorium Estoniae

Riikoja, Hindrek

2004-01-01

Majandus- ja kommunikatsiooniminister Meelis Atoneni plaanist anda praamiliinid mõne riigifirma opereerida. Vt. samas lühiintervjuud Meelis Atoneniga: Minister: riik ei piira vaba turgu. Kommenteerivad: Toivo Vaik, Andres Onemar

Obrazets stalinskoi arhitekturõ snesut / Urmas Tooming

Index Scriptorium Estoniae

Tooming, Urmas

2008-01-01

Tallinnas Narva mnt. 63 lammutatakse stalinistliku arhitektuuri üks kujukaid näiteid. Asemele ehitatakse neli ärihoonet. Kinnistu arhitektuurilise lahenduse koostas Meelis Pressi arhitektuuribüroo. Kommenteerib arhitekt Meelis Press

Spectroscopic Classification of MASTER OT J110707.62-052244.0 as a Type Ia Supernova

Science.gov (United States)

Zheng, W.; Kim, M.; Shivvers, I.; Yuk, H.; Filippenko, A. V.

2015-11-01

We report that inspection of a CCD spectrum (range 350-1050 nm) of MASTER OT J110707.62-052244.0 (ATel #8236), obtained on Nov. 11.57 UT with the Shane 3-m reflector (+ Kast spectrograph) at Lick Observatory, shows that the object is a normal Type Ia supernova roughly 1 week past maximum brightness.

78 FR 54401 - Removal of Transferred OTS Regulations Regarding Post-Employment Activities of Senior Examiners

Science.gov (United States)

2013-09-04

... F Street) on business days between 7 a.m. and 5 p.m. Please include your name, affiliation, address... section 6303(b) of the Intelligence Reform and Terrorism Prevention Act of 2004.\\4\\ This Act added a new... showing of good cause. After comparing the FDIC's rules with the transferred OTS rule relating to post...

"Käed segasid tühjalt kaarte ikka edasi, kuigi ise olin juba Une-Mati kaisus" / Urmas Vahe

Index Scriptorium Estoniae

Vahe, Urmas

2007-01-01

Maag Meelis Kubo, kes alustab ajalehes oma võlukunsti tutvustava rubriigiga. Lisaks lühitutvustus "Kes on Meelis Kubo?", sissejuhatus artikliteseeriasse "Kuidas ise mustkunsti teha?" ja esimene trikk : "Kas su isa on elektrik?"

Caracterización de la comunidad de macroinvertebrados de la Quebrada Paloblanco de la Cuenca del Río Otún (Risaralda, Colombia)

OpenAIRE

Eleonora Bernal Pinilla; Duberney García García; Miguel Adriano Novoa Bravo; Atticus Pinzón Rodríguez

2006-01-01

Se realizó un estudio de las comunidades de macroinvertebrados presentes en la quebrada Paloblanco, ubicada en la cuenca del río Otún (Risaralda, Colombia). Los puntos de muestreo seleccionados corresponden a la cabecera y la zona cercana a la desembocadura en el río Otún, ambos con características geomorfológicas de corrientes de primer orden. En cada punto se colectaron los macroinvertebrados de las unidades funcionales hojarasca, epiliton, musgo y grava, junto con una muestra de deriva y f...

Leczenie otępienia przy współistniejących schorzeniach internistycznych – wybrane zagadnienia

Directory of Open Access Journals (Sweden)

Alicja Klich-Rączka

2012-12-01

Full Text Available Celem poniższej pracy było przedstawienie dylematów związanych ze stosowaniem leków poprawiających funkcje poznawcze przy obecności innych schorzeń u ludzi starszych. Ze względu na powszechną w starości polipatologię otępienie, będące jednym z najistotniejszych problemów medycznych w tym okresie życia, rzadko występuje jako pojedyncze schorzenie – zwykle współistnieje z innymi chorobami. Leczenie otępienia jest zwykle połączone z terapią innych jednostek chorobowych, co wiąże się ze zwiększonym ryzykiem interakcji lekowych oraz działań niepożądanych. Dodatkowo ryzyko tych działań wzrasta z powodu zmian w farmakokinetyce wynikających z gorszej dystrybucji leków w organizmie oraz pogarszającej się z wiekiem wydolności wielonarządowej, czego konsekwencją może być upośledzony metabolizm i wydalanie leków. Spośród stosowanych w leczeniu otępienia inhibitorów acetylocholinesterazy interakcje najczęściej dotyczą donepezilu, co spowodowane jest jego metabolizmem w wątrobie przy udziale cytochromu P450, który równocześnie uczestniczy w metabolizmie wielu innych leków wykorzystywanych w terapii różnych schorzeń wieku starczego. Przeciwwskazaniami do stosowania inhibitorów acetylocholinesterazy są przede wszystkim: ciężka niewydolność wątroby, zespół chorego węzła zatokowego, zaburzenia rytmu i przewodnictwa, czynna choroba wrzodowa żołądka lub dwunastnicy, choroby powodujące utrudnienie odpływu moczu, napady drgawkowe, astma oskrzelowa/przewlekła obturacyjna choroba płuc. Działania niepożądane leków występują u kilku do kilkudziesięciu procent pacjentów, a u części z nich stanowią przeciwwskazania do kontynuowania terapii. Problem interakcji lekowych i działań niepożądanych jest często marginalizowany w leczeniu starszych pacjentów z otępieniem.

MASTER OT J014638.27+041324.4 is a Young Type IIP Supernova

Science.gov (United States)

Zheng, W.; Kelly, P. L.; Clubb, K. I.; Filippenko, A. V.

2013-12-01

We report that a CCD spectrum (range 350-1000 nm) of MASTER OT J014638.27+041324.4 (Shurpakov et al., ATel #5630) was obtained on Dec 6.5 UT with the Shane 3-m reflector (+Kast spectrograph) at Lick Observatory. The spectrum shows a blue continuum and weak, broad hydrogen Balmer lines having P-Cyg profiles, indicating that the object is a young Type IIP supernova. Weak He I 587.6 nm is also present.

Keratin14 mRNA expression in human pneumocytes during quiescence, repair and disease.

Directory of Open Access Journals (Sweden)

Marco Confalonieri

Full Text Available The lung alveoli slowly self-renew pneumocytes, but their facultative regeneration capacity is rapidly efficient after an injury, so fibrosis infrequently occurs. We recently observed Keratin 14 (KRT14 expression during diffuse alveolar damage (DAD, but not in controls. We wonder if KRT14 may be a marker of pneumocyte transition from quiescence to regeneration. Quantitative PCR and Western blot analyses highlighted the presence of KRT14 (mRNA and protein only in human lung samples with DAD or interstitial lung disease (ILD. In the exponentially growing cell lines A549 and H441, the mRNA and protein levels of KRT14 peaked at day one after cell seeding and decreased at day two, opposite to what observed for the proliferation marker E2F1. The inverse relation of KRT14 versus E2F1 expression holds true also for other proliferative markers, such as cyclin E1 and cyclin D1. Of interest, we also found that E2F1 silencing caused cell cycle arrest and increased KRT14 expression, whilst E2F1 stimulation induced cell cycle progression and decreased KRT14. KRT14 also increased in proliferative pneumocytes (HPAEpiC just before transdifferentiation. Overall, our results suggest that KRT14 is a viable biomarker of pneumocyte activation, and repair/regeneration. The involvement of KRT14 in regenerative process may suggest a novel pharmaceutical target to accelerate lung repair.

Eesti etümoloogia sõnaraamat / Gábor Zaicz

Index Scriptorium Estoniae

Zaicz, Gábor

2013-01-01

Arvustus: Metsmägi, Iris, Sedrik, Meeli, Soosaar, Sven-Erik. Eesti etümoloogia sõnaraamat. Peatoimetaja Iris Metsmägi. Koostanud ja toimetanud Iris Metsmägi, Meeli Sedrik, Sven-Erik Soosaar. Eesti Keele Sihtasutus. Tallinn, 2012

Avatud büroo viiendal korrusel / Maris Takk

Index Scriptorium Estoniae

Takk, Maris

2006-01-01

Tallinnas Endla t. 16 asuva Elioni pekontori sisekujundus. Autorid: Kärt Kukkur, Meelis Press. Ehitus: As Esmar Ehitus. Kärt Kukkurist ja Meelis Pressist, nende tähtsamad tööd. Ill.: plaan, 9 värv. vaadet

Computer software design description for the Treated Effluent Disposal Facility (TEDF), Project L-045H, Operator Training Station (OTS)

International Nuclear Information System (INIS)

Carter, R.L. Jr.

1994-01-01

The Treated Effluent Disposal Facility (TEDF) Operator Training Station (OTS) is a computer-based training tool designed to aid plant operations and engineering staff in familiarizing themselves with the TEDF Central Control System (CCS)

Luksuslik ja atraktiivne klaasikunst

Index Scriptorium Estoniae

2010-01-01

Eesti klaasikunstnik Meeli Kõiva tutvustab klaasi kasutamise võimalusi. Klaasimaalist, vitraažist. Meeli Kõiva klaas-valgusskulptuur "Feuille Qui Vole" ("Kohtumine lendavate lehtede all") asub Glaston Corporation'i juhtkonna hoone fuajees Soomes, "Reactive River" Euroopa Parlamendi peahoones Brüsselis

Pharmacokinetic Study of Bioactive Flavonoids in the Traditional Japanese Medicine Keigairengyoto Exerting Antibacterial Effects against Staphylococcus aureus

Directory of Open Access Journals (Sweden)

Takashi Matsumoto

2018-01-01

Full Text Available Recent studies have demonstrated that flavonoid glucuronides can be deconjugated to the active form aglycone by β-glucuronidase-expressing macrophages. Keigairengyoto (KRT is a flavonoid-rich traditional Japanese medicine reported to enhance bacterial clearance through immune modulation. Our aims are to examine the pharmacokinetics of KRT flavonoids and to identify active flavonoids contributing to the adjuvant effects of KRT. KRT was evaluated at pharmacokinetic analysis to quantify absorbed flavonoids, and cutaneous infection assay induced in mice by inoculation of Staphylococcus aureus. Preventive or therapeutic KRT administration reduced the number of bacteria in the infection site as well as macroscopic and microscopic lesion scores with efficacies similar to antibiotics. Pharmacokinetic study revealed low plasma levels of flavonoid aglycones after KRT administration; however, plasma concentrations were enhanced markedly by β-glucuronidase treatment, with baicalein the most abundant (Cmax, 1.32 µg/mL. In random screening assays, flavonoids such as bacalein, genistein, and apigenin enhanced bacteria phagocytosis by macrophages. Glucuronide bacalin was converted to aglycone baicalein by incubation with living macrophages, macrophage lysate, or skin homogenate. Taken together, the adjuvant effect of KRT may be due to some blood-absorbed flavonoids which enhance macrophage functions in host defense. Flavonoid-rich KRT may be a beneficial treatment for infectious skin inflammation.

Keratins K2 and K10 are essential for the epidermal integrity of plantar skin.

Science.gov (United States)

Fischer, Heinz; Langbein, Lutz; Reichelt, Julia; Buchberger, Maria; Tschachler, Erwin; Eckhart, Leopold

2016-01-01

K1 and K2 are the main type II keratins in the suprabasal epidermis where each of them heterodimerizes with the type I keratin K10 to form intermediate filaments. In regions of the ears, tail, and soles of the mouse, only K2 is co-expressed with K10, suggesting that these keratins suffice to form a mechanically resilient cytoskeleton. To determine the effects of the suppression of both main keratins, K2 and K10, in the suprabasal plantar epidermis of the mouse. Krt2(-/-) Krt10(-/-) mice were generated by crossing Krt2(-/-) and Krt10(-/-) mice. Epidermal morphology of soles of hind-paws was examined macroscopically and histologically. Immunofluorescence analysis and quantitative PCR analysis were performed to analyze the expression of keratins in sole skin of wildtype and Krt2(-/-) Krt10(-/-) mice. Highly abundant proteins of the sole stratum corneum were determined by electrophoretic and chromatographic separation and subsequent mass spectrometry. K2 and K10 are the most prominent suprabasal keratins in normal mouse soles with the exception of the footpads where K1, K9 and K10 predominate. Mice lacking both K2 and K10 were viable and developed epidermal acanthosis and hyperkeratosis in inter-footpad epidermis of the soles. The expression of keratins K1, K9 and K16 was massively increased at the RNA and protein levels in the soles of Krt2(-/-) Krt10(-/-) mice. This study demonstrates that the loss of the main cytoskeletal components of plantar epidermis, i.e. K2 and K10, can be only partly compensated by the upregulation of other keratins. The thickening of the epidermis in the soles of Krt2(-/-) Krt10(-/-) mice may serve as a model for pathomechanistic aspects of palmoplantar keratoderma. Copyright © 2015. Published by Elsevier Ireland Ltd.

Life-cycle and genome of OtV5, a large DNA virus of the pelagic marine unicellular green alga Ostreococcus tauri.

Directory of Open Access Journals (Sweden)

Evelyne Derelle

Full Text Available Large DNA viruses are ubiquitous, infecting diverse organisms ranging from algae to man, and have probably evolved from an ancient common ancestor. In aquatic environments, such algal viruses control blooms and shape the evolution of biodiversity in phytoplankton, but little is known about their biological functions. We show that Ostreococcus tauri, the smallest known marine photosynthetic eukaryote, whose genome is completely characterized, is a host for large DNA viruses, and present an analysis of the life-cycle and 186,234 bp long linear genome of OtV5. OtV5 is a lytic phycodnavirus which unexpectedly does not degrade its host chromosomes before the host cell bursts. Analysis of its complete genome sequence confirmed that it lacks expected site-specific endonucleases, and revealed the presence of 16 genes whose predicted functions are novel to this group of viruses. OtV5 carries at least one predicted gene whose protein closely resembles its host counterpart and several other host-like sequences, suggesting that horizontal gene transfers between host and viral genomes may occur frequently on an evolutionary scale. Fifty seven percent of the 268 predicted proteins present no similarities with any known protein in Genbank, underlining the wealth of undiscovered biological diversity present in oceanic viruses, which are estimated to harbour 200Mt of carbon.

12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

Science.gov (United States)

2010-01-01

... for a savings and loan holding company? 502.29 Section 502.29 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND FEES Assessments Savings and Loan Holding Companies-Calculation of Assessments § 502.29 How does OTS determine the condition component for a savings and loan...

12 CFR 502.26 - How does OTS calculate the semi-annual assessment for savings and loan holding companies?

Science.gov (United States)

2010-01-01

... assessment for savings and loan holding companies? 502.26 Section 502.26 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND FEES Assessments Savings and Loan Holding Companies-Calculation of Assessments § 502.26 How does OTS calculate the semi-annual assessment for savings and loan...

Gastrointestinal differentiation marker Cytokeratin 20 is regulated by homeobox gene CDX1

DEFF Research Database (Denmark)

Chan, Carol W M; Wong, Newton A; Liu, Ying

2009-01-01

colorectal cancer cell lines. Deletion and mutation analysis of the KRT20 promoter showed that the minimum regulatory region for the control of KRT20 expression by CDX1 is within 246 bp upstream of the KRT20 transcription start site. ChIP analysis confirmed that CDX1 binds to the predicted CDX elements...... in this region of the KRT20 promoter in vivo. In addition, immunohistochemistry showed expression of CDX1 parallels that of KRT20 in the normal crypt, which further supports their close relationship. In summary, our observations strongly imply that KRT20 is directly regulated by CDX1, and therefore suggest...... a role for CDX1 in maintaining differentiation in intestinal epithelial cells. Because a key feature of the development of a cancer is an unbalanced program of proliferation and differentiation, dysregulation of CDX1 may be an advantage for the development of a colorectal carcinoma. This could, therefore...

Kas ülikoolis õppimine on õige aeg lapse sünnitamiseks? / Ilona Opaal, Liis-Erliken Vinne, Tõnu Ots ... [jt.

Index Scriptorium Estoniae

2008-01-01

Küsimusele vastavad TLÜ anglistikaüliõpilane Ilona Opaal, TLÜ bioloogiaüliõpilane Liis-Erliken Vimme, psühholoog Tõnu Ots, filosoof Valdar Parve, Soome Instituudi direktor Jaana Vasama, psühholoog Tiia Lister, Treffneri Gümnaasiumi usu- ja filosoofiaõpetaja Toomas Jürgenstein

Entraining IDyOT: timing in the information dynamics of thinking

Directory of Open Access Journals (Sweden)

Jamie Forth

2016-10-01

Full Text Available We present a novel hypothetical account of entrainment in music and language, in context of the Information Dynamics of Thinking model, IDyOT. The extended model affords an alternative view of entrainment, and its companion term, pulse, from earlier accounts. The model is based on hiearchical, statistical prediction, modeling expectations of both what an event will be and when it will happen. As such,it constitutes a kind of predictive coding, with a particular novel hypothetical implementation. Here, we focus on the model’s mechanism for predicting when a perceptual event will happen, given an existing sequence of past events, which maybe musical or linguistic. We propose a range of tests to validate or falsify the model, at various different levels of abstraction, and argue that computational modelling in particular, and this model in particular, can offer a means of providing limited but useful evidence for evolutionary hypotheses.

Consonant harmony in Nilotic: contrastive specifications and Stratal OT

Directory of Open Access Journals (Sweden)

Sara Mackenzie

2016-07-01

Full Text Available Through analyses of dental harmony in four Nilotic languages, this paper argues both for contrastive representations and for a stratal version of Optimality Theory. Dholuo, Päri, Shilluk and Anywa all have dental harmony patterns which bar the co-occurrence of dental and alveolar segments. Details of the dental harmony patterning vary between languages and are tied to contrasts in the inventory. In Anywa, harmony is non-structure-preserving and an allophonic dental nasal surfaces in harmonic forms. This pattern can be analyzed in a stratal model of OT but leads to a ranking paradox if only a single level of evaluation is used. Further support for a stratal analysis can be found in the interaction between harmony and consonant mutation processes in the four languages. In Dholuo, harmony applies at the stem level and consonant mutation results in disharmonic surface forms in morphologically complex words. In Anywa, harmony applies later, at the word level, and words formed through consonant mutation must obey the dental harmony restrictions.

Spatiotemporal analysis of putative notochordal cell markers reveals CD24 and keratins 8, 18, and 19 as notochord-specific markers during early human intervertebral disc development.

Science.gov (United States)

Rodrigues-Pinto, Ricardo; Berry, Andrew; Piper-Hanley, Karen; Hanley, Neil; Richardson, Stephen M; Hoyland, Judith A

2016-08-01

In humans, the nucleus pulposus (NP) is composed of large vacuolated notochordal cells in the fetus but, soon after birth, becomes populated by smaller, chondrocyte-like cells. Although animal studies indicate that notochord-derived cells persist in the adult NP, the ontogeny of the adult human NP cell population is still unclear. As such, identification of unique notochordal markers is required. This study was conducted to determine the spatiotemporal expression of putative human notochordal markers to aid in the elucidation of the ontogeny of adult human NP cells. Human embryos and fetuses (3.5-18 weeks post-conception (WPC)) were microdissected to isolate the spine anlagens (notochord and somites/sclerotome). Morphology of the developing IVD was assessed using hematoxylin and eosin. Expression of keratin (KRT) 8, KRT18, KRT19, CD24, GAL3, CD55, BASP1, CTGF, T, CD90, Tie2, and E-cadherin was assessed using immunohistochemistry. KRT8, KRT18, KRT19 were uniquely expressed by notochordal cells at all spine levels at all stages studied; CD24 was expressed at all stages except 3.5 WPC. While GAL3, CD55, BASP1, CTGF, and T were expressed by notochordal cells at specific stages, they were also co-expressed by sclerotomal cells. CD90, Tie2, and E-cadherin expression was not detectable in developing human spine cells at any stage. This study has identified, for the first time, the consistent expression of KRT8, KRT18, KRT19, and CD24 as human notochord-specific markers during early IVD development. Thus, we propose that these markers can be used to help ascertain the ontogeny of adult human NP cells. © 2016 The Authors. Journal of Orthopaedic Research Published by Wiley Periodicals, Inc. J Orthop Res 34:1327-1340, 2016. © 2016 The Authors. Journal of Orthopaedic Research Published by Wiley Periodicals, Inc.

Spatiotemporal analysis of putative notochordal cell markers reveals CD24 and keratins 8, 18, and 19 as notochord‐specific markers during early human intervertebral disc development

Science.gov (United States)

Rodrigues‐Pinto, Ricardo; Berry, Andrew; Piper‐Hanley, Karen; Hanley, Neil; Richardson, Stephen M.

2016-01-01

ABSTRACT In humans, the nucleus pulposus (NP) is composed of large vacuolated notochordal cells in the fetus but, soon after birth, becomes populated by smaller, chondrocyte‐like cells. Although animal studies indicate that notochord‐derived cells persist in the adult NP, the ontogeny of the adult human NP cell population is still unclear. As such, identification of unique notochordal markers is required. This study was conducted to determine the spatiotemporal expression of putative human notochordal markers to aid in the elucidation of the ontogeny of adult human NP cells. Human embryos and fetuses (3.5–18 weeks post‐conception (WPC)) were microdissected to isolate the spine anlagens (notochord and somites/sclerotome). Morphology of the developing IVD was assessed using hematoxylin and eosin. Expression of keratin (KRT) 8, KRT18, KRT19, CD24, GAL3, CD55, BASP1, CTGF, T, CD90, Tie2, and E‐cadherin was assessed using immunohistochemistry. KRT8, KRT18, KRT19 were uniquely expressed by notochordal cells at all spine levels at all stages studied; CD24 was expressed at all stages except 3.5 WPC. While GAL3, CD55, BASP1, CTGF, and T were expressed by notochordal cells at specific stages, they were also co‐expressed by sclerotomal cells. CD90, Tie2, and E‐cadherin expression was not detectable in developing human spine cells at any stage. This study has identified, for the first time, the consistent expression of KRT8, KRT18, KRT19, and CD24 as human notochord‐specific markers during early IVD development. Thus, we propose that these markers can be used to help ascertain the ontogeny of adult human NP cells. © 2016 The Authors. Journal of Orthopaedic Research Published by Wiley Periodicals, Inc. J Orthop Res 34:1327–1340, 2016. PMID:26910849

Cytokeratin expression in mouse lacrimal gland germ epithelium.

Science.gov (United States)

Hirayama, Masatoshi; Liu, Ying; Kawakita, Tetsuya; Shimmura, Shigeto; Tsubota, Kazuo

2016-05-01

The lacrimal gland secretes tear fluids that protect the ocular surface epithelium, and its dysfunction leads to dry eye disease (DED). The functional restoration of the lacrimal gland by engraftment of a bioengineered lacrimal gland using lacrimal gland germ epithelial cells has been proposed to cure DED in mice. Here, we investigate the expression profile of cytokeratins in the lacrimal gland germ epithelium to clarify their unique characteristics. We performed quantitative polymerase chain reaction (Q-PCR) and immunohistochemistry (IHC) analysis to clarify the expression profile of cytokeratin in the lacrimal gland germ epithelium. The mRNA expression of keratin (KRT) 5, KRT8, KRT14, KRT15, and KRT18 in the lacrimal gland germ epithelium was increased compared with that in mouse embryonic stem cells and the lacrimal gland germ mesenchyme, as analyzed by Q-PCR. The expression level of KRT15 increased in the transition from stem cells to lacrimal gland germ epithelium, then decreased as the lacrimal gland matured. IHC revealed that the expression set of these cytokeratins in the lacrimal gland germ epithelium was different from that in the adult lacrimal gland. The expression of KRT15 was observed in the lacrimal gland germ epithelium, and it segmentalized into some of the basal cells in the intercanulated duct in mature gland. We determined the expression profile of cytokeratins in the lacrimal gland epithelium, and identified KRT15 as a candidate unique cellular marker for the lacrimal gland germ epithelium. Copyright © 2015 Elsevier Ltd. All rights reserved.

Hıyarda (Cucumis sativus L. yabancı ot çıkış zamanın tahminine yönelik araştırmalar

Directory of Open Access Journals (Sweden)

Nihat Tursun

2015-01-01

Full Text Available İstatistiki modellerin geliştirilmesi için hıyar bitkisinde on bir önemli yabancı otun çıkış zamanın belirlenmesinde modellemeler yapılmıştır. Hıyar deneme arazisinde bulunan önemli yabancı otlar çıkış zamanlarına göre gruplara ayrılmıştır. Bu yabancı otlardan; Amaranthus retroflexus, Chenopodium album, Heliotropium europaeum, Polygonum aviculare ve Solanum nigrum erken, Convolvulus arvensis, Cyperus rotundus, Cynodon dactylon, Portulaca oleracea ve Sorghum halepense bütün vejetasyon boyunca ve Tribulus terrestris ise geç çimlenen yabancı ot türü olarak belirlenmiştir. Farklı doğrusal olmayan büyüme eğrileri (Chapman-Richard, Weibull, logistic, Gompertz ve cubic spline farklı yabancı ot türleri ve yıllar için çıkış yüzdeleri verileri esas alınarak uyarlanmıştır. Cubic splin model birçok yabancı ot türü için en iyi model olarak saptanmıştır.

Transcriptional analysis and molecular dynamics simulations reveal the mechanism of toxic metals removal and efflux pumps in Lysinibacillus sphaericus OT4b.31

KAUST Repository

Shaw, Dario Rangel; Dussan, Jenny

2017-01-01

Lysinibacillus sphaericus strain OT4b.31 is a bacterium widely applied in bioremediation processes of hydrocarbon and metal polluted environments. In this study, we identified the molecular mechanism underlying the Pb2+ and Cr6+ resistance. Metal

Purification, crystallization and preliminary X-ray diffraction studies of a putative UDP-N-acetyl-d-mannosamine dehydrogenase from Pyrococcus horikoshii OT3

Energy Technology Data Exchange (ETDEWEB)

Lokanath, Neratur K.; Pampa, Kudigana J.; Kamiya, Toshimi; Kunishima, Naoki, E-mail: kunisima@spring8.or.jp [Advanced Protein Crystallography Research Group, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148 (Japan)

2007-05-01

A putative UDP-N-acetyl-d-mannosamine dehydrogenase from P. horikoshii OT3 has been crystallized in space group P2{sub 1}, with unit-cell parameters a = 80.28, b = 69.24, c = 83.10 Å, β = 114.4°. X-ray diffraction data have been collected to 1.80 Å resolution. A putative UDP-N-acetyl-d-mannosamine dehydrogenase from Pyrococcus horikoshii OT3, an essential enzyme for polysaccharide biosynthesis, has been overexpressed in Escherichia coli and purified. Crystals were obtained using the oil-microbatch method at 291 K. A native data set extending to 1.8 Å resolution has been collected and processed in space group P2{sub 1}. Assuming the presence of a dimer in the asymmetric unit, the V{sub M} value is calculated to be 2.3 Å{sup 3} Da{sup −1}, which is consistent with the result of a dynamic light-scattering experiment that shows a dimeric state of the protein in solution.

Zastosowanie jakościowej analizy profilu wykonania skali ACE-III w diagnostyce różnicowej chorób otępiennych

Directory of Open Access Journals (Sweden)

Emilia J. Sitek

2017-03-01

Full Text Available Skala Addenbrooke’s Cognitive Examination III (ACE-III jest poszerzonym narzędziem przesiewowej oceny funkcji poznawczych, użytecznym we wczesnym wykrywaniu zaburzeń poznawczych, wstępnej diagnostyce różnicowej zespołów otępiennych oraz monitorowaniu postępu choroby. ACE-III ocenia uwagę i orientację, pamięć, fluencję słowną, funkcje językowe i wzrokowo-przestrzenne. Skala może być używana przez lekarzy i psychologów, zarówno jako narzędzie przesiewowe, jak i jako wstęp do kompleksowej oceny neuropsychologicznej. ACE-III zawiera również krótsze narzędzie, Mini-ACE, które w razie potrzeby może być stosowane niezależnie. W niniejszej pracy ACE-III porównano z innymi najbardziej popularnymi skalami przesiewowej oceny funkcji poznawczych: Mini-Mental State Examination (MMSE oraz Montrealską Skalą Oceny Funkcji Poznawczych (Montreal Cognitive Assessment, MoCA. Następnie omówiono przydatność kliniczną analizy profilu wykonania ACE-III w  diagnostyce różnicowej wybranych chorób neurozwyrodnieniowych. Zaprezentowano profile wykonania ACE-III typowe dla choroby Alzheimera (zarówno o wczesnym, jak i o późnym początku, zwyrodnienia czołowo-skroniowego, choroby Parkinsona, atypowych zespołów parkinsonowskich oraz otępienia naczyniopochodnego. Spośród atypowych wariantów choroby Alzheimera uwzględniono zanik korowy tylny, wariant logopeniczny afazji pierwotnej postępującej i wariant czołowy. Spośród podstawowych wariantów zwyrodnienia czołowo-skroniowego przedstawiono wariant behawioralny otępienia czołowo- -skroniowego oraz niepłynny i semantyczny wariant afazji pierwotnej postępującej. Wreszcie spośród zespołów parkinsonizm plus omówiono otępienie z ciałami Lewy’ego, zanik wieloukładowy, postępujące porażenie ponadjądrowe i zwyrodnienie korowo-podstawne.

Crystallization and preliminary X-ray analysis of PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins

International Nuclear Information System (INIS)

Shirokane, Michio; Sawano, Yoriko; Miyazono, Ken-ichi; Nagata, Koji; Tanokura, Masaru

2007-01-01

PH1010, a DUF54-family protein from the hyperthermophilic archaeon P. horikoshii OT3, was crystallized and X-ray diffraction data were collected to 1.90 Å resolution. PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins, was expressed, purified and crystallized. Crystallization was performed by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystal diffracted X-rays to 1.90 Å resolution using a synchrotron-radiation source. The space group of the crystal was determined to be P2 1 2 1 2 1 , with unit-cell parameters a = 46.9, b = 49.5, c = 132.7 Å. The crystal contained two PH1010 molecules in the asymmetric unit (V M = 2.4 Å 3 Da −1 ) and had a solvent content of 48%

Purification, crystallization and preliminary X-ray crystallographic analysis of the archaeal phosphoglycerate mutase PH0037 from Pyrococcus horikoshii OT3

Energy Technology Data Exchange (ETDEWEB)

Lokanath, Neratur K.; Kunishima, Naoki, E-mail: kunisima@spring8.or.jp [Advanced Protein Crystallography Research Group, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148 (Japan)

2006-08-01

The archaeal phosphoglycerate mutase PH0037 from P. horikoshii OT3 has been crystallized in space group R32, with unit-cell parameters a = 155.62, c = 230.35 Å. A 2.2 Å resolution data was collected at SPring-8 beamline BL26B1. Phosphoglycerate mutases catalyze the interconversion of 2-phosphoglycerate and 3-phosphoglycerate in glycolysis and gluconeogenesis pathways. The archaeal phosphoglycerate mutase PH0037 from Pyrococcus horikoshii OT3 has been overexpressed in Escherichia coli and purified. Crystals were obtained using the oil-microbatch method at 291 K. A native data set extending to a resolution of 2.2 Å has been collected and processed in space group R32. Assuming the presence of a dimer in the asymmetric unit, the V{sub M} value is calculated to be 3.0 Å{sup 3} Da{sup −1}, consistent with the dynamic light-scattering experiment result, which shows a dimeric state of the protein in solution. Molecular-replacement trials using the crystal structure of Bacilllus stearothermophilus phosphoglycerate mutase as a search model did not provide a satisfactory solution, indicating substantially different structures of these two phophoglycerate mutases.

Overexpression, purification, crystallization and preliminary crystallographic studies of a hyperthermophilic adenylosuccinate synthetase from Pyrococcus horikoshii OT3

International Nuclear Information System (INIS)

Wang, Xiaoying; Akasaka, Ryogo; Takemoto, Chie; Morita, Satoshi; Yamaguchi, Machiko; Terada, Takaho; Shirozu, Mikako; Yokoyama, Shigeyuki; Chen, Shilin; Si, Shuyi; Xie, Yong

2011-01-01

A hyperthermophilic adenylosuccinate synthetase from P. horikoshii OT3, which is 90–120 amino acids shorter than those from the vast majority of organisms, was expressed, purified and crystallized and X-ray diffraction data were collected to 2.5 Å resolution. Adenylosuccinate synthetase (AdSS) is a ubiquitous enzyme that catalyzes the first committed step in the conversion of inosine monophosphate (IMP) to adenosine monophosphate (AMP) in the purine-biosynthetic pathway. Although AdSS from the vast majority of organisms is 430–457 amino acids in length, AdSS sequences isolated from thermophilic archaea are 90–120 amino acids shorter. In this study, crystallographic studies of a short AdSS sequence from Pyrococcus horikoshii OT3 (PhAdSS) were performed in order to reveal the unusual structure of AdSS from thermophilic archaea. Crystals of PhAdSS were obtained by the microbatch-under-oil method and X-ray diffraction data were collected to 2.50 Å resolution. The crystal belonged to the trigonal space group P3 2 12, with unit-cell parameters a = b = 57.2, c = 107.9 Å. There was one molecule per asymmetric unit, giving a Matthews coefficient of 2.17 Å 3 Da −1 and an approximate solvent content of 43%. In contrast, the results of native polyacrylamide gel electrophoresis and analytical ultracentrifugation showed that the recombinant PhAdSS formed a dimer in solution

Conservation of Repeats at the Mammalian KCNQ1OT1-CDKN1C Region Suggests a Role in Genomic Imprinting

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Marcos De Donato

2017-06-01

Full Text Available KCNQ1OT1 is located in the region with the highest number of genes showing genomic imprinting, but the mechanisms controlling the genes under its influence have not been fully elucidated. Therefore, we conducted a comparative analysis of the KCNQ1/KCNQ1OT1-CDKN1C region to study its conservation across the best assembled eutherian mammalian genomes sequenced to date and analyzed potential elements that may be implicated in the control of genomic imprinting in this region. The genomic features in these regions from human, mouse, cattle, and dog show a higher number of genes and CpG islands (detected using cpgplot from EMBOSS, but lower number of repetitive elements (including short interspersed nuclear elements and long interspersed nuclear elements, compared with their whole chromosomes (detected by RepeatMasker. The KCNQ1OT1-CDKN1C region contains the highest number of conserved noncoding sequences (CNS among mammals, where we found 16 regions containing about 38 different highly conserved repetitive elements (using mVista, such as LINE1 elements: L1M4, L1MB7, HAL1, L1M4a, L1Med, and an LTR element: MLT1H. From these elements, we found 74 CNS showing high sequence identity (>70% between human, cattle, and mouse, from which we identified 13 motifs (using Multiple Em for Motif Elicitation/Motif Alignment and Search Tool with a significant probability of occurrence, 3 of which were the most frequent and were used to find transcription factor–binding sites. We detected several transcription factors (using JASPAR suite from the families SOX, FOX, and GATA. A phylogenetic analysis of these CNS from human, marmoset, mouse, rat, cattle, dog, horse, and elephant shows branches with high levels of support and very similar phylogenetic relationships among these groups, confirming previous reports. Our results suggest that functional DNA elements identified by comparative genomics in a region densely populated with imprinted mammalian genes may be

Crystallization and preliminary X-ray analysis of PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins

Energy Technology Data Exchange (ETDEWEB)

Shirokane, Michio; Sawano, Yoriko; Miyazono, Ken-ichi; Nagata, Koji; Tanokura, Masaru, E-mail: amtanok@mail.ecc.u-tokyo.ac.jp [Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan)

2007-06-01

PH1010, a DUF54-family protein from the hyperthermophilic archaeon P. horikoshii OT3, was crystallized and X-ray diffraction data were collected to 1.90 Å resolution. PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins, was expressed, purified and crystallized. Crystallization was performed by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystal diffracted X-rays to 1.90 Å resolution using a synchrotron-radiation source. The space group of the crystal was determined to be P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 46.9, b = 49.5, c = 132.7 Å. The crystal contained two PH1010 molecules in the asymmetric unit (V{sub M} = 2.4 Å{sup 3} Da{sup −1}) and had a solvent content of 48%.

Geología de la zona de influencia de la falla otú en el distrito minero segovia-remedios

Directory of Open Access Journals (Sweden)

MILTON ÁLVAREZ GALINDEZ

2007-01-01

Full Text Available Se presenta la caracterización geológica y estructural de la zona de influencia de la falla Otú en jurisdicción del distrito minero Segovia-Remedios. Además, se proponen controles mecánicos para las mineralizaciones auríferas, que si bien son compatibles, en parte, con un modelo de depósitos de oro orogénico, dejan abiertas otras posibles interpretaciones

A global outer-rise/outer-trench-slope (OR/OTS) earthquake study

Science.gov (United States)

Wartman, J. M.; Kita, S.; Kirby, S. H.; Choy, G. L.

2009-12-01

Using improved seismic, bathymetric, satellite gravity and other geophysical data, we investigated the seismicity patterns and focal mechanisms of earthquakes in oceanic lithosphere off the trenches of the world that are large enough to be well recorded at teleseismic distances. A number of prominent trends are apparent, some of which have been previously recognized based on more limited data [1], and some of which are largely new [2-5]: (1) The largest events and the highest seismicity rates tend to occur where Mesozoic incoming plates are subducting at high rates (e.g., those in the western Pacific and the Banda segment of Indonesia). The largest events are predominantly shallow normal faulting (SNF) earthquakes. Less common are reverse-faulting (RF) events that tend to be deeper and to be present along with SNF events where nearby seamounts, seamount chains and other volcanic features are subducting [Seno and Yamanaka, 1996]. Blooms of SNF OR/OTS events usually occur just after and seaward of great interplate thrust (IPT) earthquakes but are far less common after smaller IPT events. (2) Plates subducting at slow rates (Chile, the Ninety East Ridge in Sumatra, and the D’Entrecastaux Ridge in Vanuatu).

A Novel Role for Keratin 17 in Coordinating Oncogenic Transformation and Cellular Adhesion in Ewing Sarcoma

Science.gov (United States)

Sankar, Savita; Tanner, Jason M.; Bell, Russell; Chaturvedi, Aashi; Randall, R. Lor; Beckerle, Mary C.

2013-01-01

Oncogenic transformation in Ewing sarcoma is caused by EWS/FLI, an aberrant transcription factor fusion oncogene. Glioma-associated oncogene homolog 1 (GLI1) is a critical target gene activated by EWS/FLI, but the mechanism by which GLI1 contributes to the transformed phenotype of Ewing sarcoma was unknown. In this work, we identify keratin 17 (KRT17) as a direct downstream target gene upregulated by GLI1. We demonstrate that KRT17 regulates cellular adhesion by activating AKT/PKB (protein kinase B) signaling. In addition, KRT17 is necessary for oncogenic transformation in Ewing sarcoma and accounts for much of the GLI1-mediated transformation function but via a mechanism independent of AKT signaling. Taken together, our data reveal previously unknown molecular functions for a cytoplasmic intermediate filament protein, KRT17, in coordinating EWS/FLI- and GLI1-mediated oncogenic transformation and cellular adhesion in Ewing sarcoma. PMID:24043308

Characterization of an immunodominant cancer-specific O-glycopeptide epitope in murine podoplanin (OTS8)

DEFF Research Database (Denmark)

Steentoft, Catharina; Schjoldager, Katrine T; Cló, Emiliano

2010-01-01

antibody 237, developed to a spontaneous murine fibrosarcoma, was shown to be directed to murine podoplanin (OTS8) with truncated Tn O-glycans. Our understanding of such cancer-specific auto-antibodies to truncated glycoforms of glycoproteins is limited. Here we have investigated immunogenicity...... of a chemoenzymatically produced Tn-glycopeptide derived from the putative murine podoplanin O-glycopeptide epitope. We found that the Tn O-glycopeptide was highly immunogenic in mice and produced a Tn-glycoform specific response with no reactivity against unglycosylated peptides or the O-glycopeptide with extended O......-glycan (STn and T glycoforms). The immunodominant epitope was strictly dependent on the peptide sequence, required Tn at a specific single Thr residue (Thr(77)), and antibodies to the epitope were not found in naive mice. We further tested a Tn O-glycopeptide library derived from human podoplanin...

Testosterone regulates keratin 33B expression in rat penis growth through androgen receptor signaling.

Science.gov (United States)

Ma, Yan-Min; Wu, Kai-Jie; Dang, Qiang; Shi, Qi; Gao, Yang; Guo, Peng; Xu, Shan; Wang, Xin-Yang; He, Da-Lin; Gong, Yong-Guang

2014-01-01

Androgen therapy is the mainstay of treatment for the hypogonadotropic hypogonadal micropenis because it obviously enhances penis growth in prepubescent microphallic patients. However, the molecular mechanisms of androgen treatment leading to penis growth are still largely unknown. To clarify this well-known phenomenon, we successfully generated a castrated male Sprague Dawley rat model at puberty followed by testosterone administration. Interestingly, compared with the control group, testosterone treatment stimulated a dose-dependent increase of penis weight, length, and width in castrated rats accompanied with a dramatic recovery of the pathological changes of the penis. Mechanistically, testosterone administration substantially increased the expression of androgen receptor (AR) protein. Increased AR protein in the penis could subsequently initiate transcription of its target genes, including keratin 33B (Krt33b). Importantly, we demonstrated that KRT33B is generally expressed in the rat penis and that most KRT33B expression is cytoplasmic. Furthermore, AR could directly modulate its expression by binding to a putative androgen response element sequence of the Krt33b promoter. Overall, this study reveals a novel mechanism facilitating penis growth after testosterone treatment in precastrated prepubescent animals, in which androgen enhances the expression of AR protein as well as its target genes, such as Krt33b.

Testosterone regulates keratin 33B expression in rat penis growth through androgen receptor signaling

Directory of Open Access Journals (Sweden)

Yan-Min Ma

2014-12-01

Full Text Available Androgen therapy is the mainstay of treatment for the hypogonadotropic hypogonadal micropenis because it obviously enhances penis growth in prepubescent microphallic patients. However, the molecular mechanisms of androgen treatment leading to penis growth are still largely unknown. To clarify this well-known phenomenon, we successfully generated a castrated male Sprague Dawley rat model at puberty followed by testosterone administration. Interestingly, compared with the control group, testosterone treatment stimulated a dose-dependent increase of penis weight, length, and width in castrated rats accompanied with a dramatic recovery of the pathological changes of the penis. Mechanistically, testosterone administration substantially increased the expression of androgen receptor (AR protein. Increased AR protein in the penis could subsequently initiate transcription of its target genes, including keratin 33B (Krt33b. Importantly, we demonstrated that KRT33B is generally expressed in the rat penis and that most KRT33B expression is cytoplasmic. Furthermore, AR could directly modulate its expression by binding to a putative androgen response element sequence of the Krt33b promoter. Overall, this study reveals a novel mechanism facilitating penis growth after testosterone treatment in precastrated prepubescent animals, in which androgen enhances the expression of AR protein as well as its target genes, such as Krt33b.

A Otávia do Pseudo-Sêneca: tradução, estudo introdutório e notas

OpenAIRE

Fernanda Vieira Gôzo

2016-01-01

Esta pesquisa de mestrado objetivou o estudo da peça latina Otávia do Pseudo-Sêneca, que inclui uma tradução para o português, acompanhada de notas e de um estudo introdutório. Este trabalho centrou-se em apontar o que se têm discutido entre os estudiosos de literatura clássica em relação à peça. Foi pertinente para esse expediente uma análise do gênero praetexta, do qual faz parte esta peça, considerando as divergências entre praetexta republicana e imperial, bem como a observação de como a ...

Databáze a generování testovacích otázek

OpenAIRE

Rejko, Peter

2008-01-01

Cílem bakalářské práce je vytvoření databáze testovacích otázek a jejich generování. Je rozdělená na teoretickou a praktickou část. V teoretické jsou popsané nástroje se zaměřením na tvorbu databází jazyku HTML, PHP a databázový server MySQL. Praktická část obsahuje vytvořenou aplikaci a zpracování. Jazyk PHP podporuje programování na základě modulárního paradigmatu - to znamená, že každý vytvořený modul má svoji úlohu. Aplikace obsahuje modul zabezpečující přihlášení a výběr dalšího modulu n...

Evaluación de la diversidad de bacterias degradadoras de hidrocarburos aisladas de suelos de las cuencas de los ríos Otún y La Vieja / Evaluation of hydrocarbon degrading bacteria diversity isolated from soils of Otún and La Vieja river basins

OpenAIRE

Yanine Suárez, Habib Fernando

2010-01-01

Se evaluó el efecto del uso del suelo, sobre la densidad y diversidad de bacterias degradadoras de hidrocarburos (HC). Se seleccionaron los usos: bosque, pastizal y cafetal en la cuenca del río La Vieja (Valle del Cauca y Quindío), y bosque, cebollar y plantación forestal en la cuenca del río Otún (Risaralda) y se realizaron dos eventos de muestreo. La densidad se evaluó mediante la técnica de NMP en medio Bushnell-Hass, suplementado con diesel y usando XTT como indicador de actividad. Las ba...

A genome-wide association study of bipolar disorder with comorbid eating disorder replicates the SOX2-OT region.

Science.gov (United States)

Liu, Xiaohua; Kelsoe, John R; Greenwood, Tiffany A

2016-01-01

Bipolar disorder is a heterogeneous mood disorder associated with several important clinical comorbidities, such as eating disorders. This clinical heterogeneity complicates the identification of genetic variants contributing to bipolar susceptibility. Here we investigate comorbidity of eating disorders as a subphenotype of bipolar disorder to identify genetic variation that is common and unique to both disorders. We performed a genome-wide association analysis contrasting 184 bipolar subjects with eating disorder comorbidity against both 1370 controls and 2006 subjects with bipolar disorder only from the Bipolar Genome Study (BiGS). The most significant genome-wide finding was observed bipolar with comorbid eating disorder vs. controls within SOX2-OT (p=8.9×10(-8) for rs4854912) with a secondary peak in the adjacent FXR1 gene (p=1.2×10(-6) for rs1805576) on chromosome 3q26.33. This region was also the most prominent finding in the case-only analysis (p=3.5×10(-7) and 4.3×10(-6), respectively). Several regions of interest containing genes involved in neurodevelopment and neuroprotection processes were also identified. While our primary finding did not quite reach genome-wide significance, likely due to the relatively limited sample size, these results can be viewed as a replication of a recent study of eating disorders in a large cohort. These findings replicate the prior association of SOX2-OT with eating disorders and broadly support the involvement of neurodevelopmental/neuroprotective mechanisms in the pathophysiology of both disorders. They further suggest that different clinical manifestations of bipolar disorder may reflect differential genetic contributions and argue for the utility of clinical subphenotypes in identifying additional molecular pathways leading to illness. Copyright © 2015 Elsevier B.V. All rights reserved.

Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells.

Directory of Open Access Journals (Sweden)

Satoru Yoshida

Full Text Available BACKGROUND: Application of induced pluripotent stem (iPS cells in regenerative medicine will bypass ethical issues associated with use of embryonic stem cells. In addition, patient-specific IPS cells can be useful to elucidate the pathophysiology of genetic disorders, drug screening, and tailor-made medicine. However, in order to apply iPS cells to mitotic tissue, induction of tissue stem cells that give rise to progeny of the target organ is required. METHODOLOGY/PRINCIPAL FINDINGS: We induced stratified epithelial cells from mouse iPS cells by co-culture with PA6 feeder cells (SDIA-method with use of BMP4. Clusters of cells positive for the differentiation markers KRT1 or KRT12 were observed in KRT14-positive colonies. We successfully cloned KRT14 and p63 double-positive stratified epithelial progenitor cells from iPS-derived epithelial cells, which formed stratified epithelial sheets consisting of five- to six-polarized epithelial cells in vitro. When these clonal cells were cultured on denuded mouse corneas, a robust stratified epithelial layer was observed with physiological cell polarity including high levels of E-cadherin, p63 and K15 expression in the basal layer and ZO-1 in the superficial layer, recapitulating the apico-basal polarity of the epithelium in vivo. CONCLUSIONS/SIGNIFICANCE: These results suggest that KRT14 and p63 double-positive epithelial progenitor cells can be cloned from iPS cells in order to produce polarized multilayer epithelial cell sheets.

Evaluation of a Coaching Experiential Learning Project on OT Student Abilities and Perceptions

Directory of Open Access Journals (Sweden)

Erin A. Phillips

2017-01-01

Full Text Available Innovative teaching methods to address emerging practice needs are critical components of effective occupational therapy education. Experiential learning strategies can enhance skill development and translation of knowledge into OT clinical practice. In addition, skills such as coaching may provide important links to health promotion practices. Thirty-two occupational therapy students took part in an experiential project to connect occupational engagement and health for a community of older adults. A pretest/posttest design was used to evaluate program outcomes in student perceived abilities, and narrative reflection papers provided postexperience qualitative information. The students improved in all 10 areas of abilities selfassessment with mean total scores from pretest (M = 42 improving significantly at posttest (M = 58. Themes from reflection papers indicated a positive response to experiential learning and a desire for more opportunities to prepare for clinical practice, including the use of interprofessional training. The students improved in their abilities to use coaching and health promotion strategies through the use of experiential learning methods. Outcomes suggest that experiential learning opportunities are an effective way to enhance student competencies in coaching, improve readiness for wellness programming, and increase student confidence in application of skills in future clinical practice.

Caracterización de la comunidad de macroinvertebrados de la Quebrada Paloblanco de la Cuenca del Río Otún (Risaralda, Colombia

Directory of Open Access Journals (Sweden)

Eleonora Bernal Pinilla

2006-07-01

Full Text Available Se realizó un estudio de las comunidades de macroinvertebrados presentes en la quebrada Paloblanco, ubicada en la cuenca del río Otún (Risaralda, Colombia. Los puntos de muestreo seleccionados corresponden a la cabecera y la zona cercana a la desembocadura en el río Otún, ambos con características geomorfológicas de corrientes de primer orden. En cada punto se colectaron los macroinvertebrados de las unidades funcionales hojarasca, epiliton, musgo y grava, junto con una muestra de deriva y fauna general (sin discriminar unidad funcional. Se encontraron 42 familias de Insecta, además de Acari, Annelida, Crustacea e Hirudinea. Igualmente se encontraron familias de presencia exclusiva en cada punto, mostrando diferencias en la composición de las comunidades. Los índices ecológicos empleados mostraron una mayor diversidad en punto bajo y diferencias entre algunas de las unidades funcionales, relacionados con el medio y los hábitos presentes. Para el análisis se tuvieron en cuenta los conceptos de continuo y zonación de los ríos. A pesar de tener condiciones de primer orden, existen variaciones a pequeña escala entre los puntos de muestreo, que están relacionadas con la intervención antrópica en la zona aledaña a punto bajo.

Set dlja perevoztshikov / Aleksei Zhigulin

Index Scriptorium Estoniae

Zhigulin, Aleksei

2004-01-01

Majandus- ja kommunikatsiooniminister Meelis Atoneni osalemisel toimus ümarlaud, mis arutas Eesti merekaubalaevanduse edaspidist arengut Euroopa Liidu tingimustes, loomisel on lähimerevedude edendamiskeskus

Kuumav disain / Kristjan Arunurm

Index Scriptorium Estoniae

Arunurm, Kristjan

2008-01-01

Eestis pakutavatest disainradiaatoritest, soovitusi radiaatori ostjatele. Kommenteerivad Zehnder esindaja Eestis Anneli Saal, Tatjana Mihhailova Radiaatorikeskusest, Meelis Saal firmast Vikingland ja Aivar Kahr firmast Airmate

OT2_smalhotr_3: Herschel Extreme Lensing Line Observations (HELLO)

Science.gov (United States)

Malhotra, S.

2011-09-01

We request 59.8 hours of Herschel time to observe 20 normal star-forming galaxies in the [CII] 158 micron and [OI] 63 micron lines. These galaxies lie at high redshift (1OT1, 49 high redshift IR luminous galaxies were approved for spectroscopy, but only two so-called normal galaxies were included. This is an imbalance that should be corrected, to balance Herschel's legacy.

Farmakoterapia zaburzeń behawioralnych towarzyszących otępieniu

Directory of Open Access Journals (Sweden)

Tomasz Sobów

2013-12-01

Full Text Available Farmakoterapia zaburzeń zachowania i objawów neuropsychiatrycznych u chorych otępiałych (behavioural and psychological symptoms of dementia, BPSD była przedmiotem wielu badań klinicznych. Ich wyniki nie pozwalają niestety na sformułowanie prostych zaleceń. Leki przeciwpsychotyczne (zwłaszcza II generacji okazały się względnie skuteczne w zwalczaniu pobudzenia i agresji, ale ich działanie na objawy psychotyczne jest wątpliwe. Poważne obawy dotyczące bezpieczeństwa stosowania leków przeciwpsychotycznych (zwłaszcza ryzyko ostrych zdarzeń sercowo-mózgowych i związanej z nimi śmiertelności w znaczący sposób ograniczają ich wykorzystanie. Leki przeciwdepresyjne, szczególnie z grupy selektywnych inhibitorów zwrotnego wychwytu serotoniny (selective serotonin reuptake inhibitor, SSRI, wykazują podobną skuteczność w zwalczaniu pobudzenia i objawów psychotycznych jak leki przeciwpsychotyczne, co wobec lepszej tolerancji zachęca do ich szerszego stosowania. Inne grupy leków (w tym przeciwdrgawkowe i benzodiazepiny, pomimo teoretycznych przesłanek sugerujących możliwą skuteczność, w randomizowanych badaniach nie zostały ocenione jako wystarczająco efektywne (i bezpieczne, aby rekomendować ich szersze wykorzystanie. Rutynowe stosowanie zasady hierarchicznego postępowania w BPSD (najpierw staranna diagnostyka, w tym wykluczenie majaczenia, następnie interwencje niefarmakologiczne i optymalizacja leczenia inhibitorami cholinesterazy i/lub memantyną, a dopiero w trzeciej kolejności zastosowanie leków psychotropowych pozwala na ograniczenie farmakoterapii w tej szczególnie wrażliwej grupie pacjentów.

Keratin 23, a novel DPC4/Smad4 target gene which binds 14-3-3ε

International Nuclear Information System (INIS)

Liffers, Sven-T; Schwarte-Waldhoff, Irmgard; Meyer, Helmut E; Stühler, Kai; Hahn, Stephan A; Maghnouj, Abdelouahid; Munding, Johanna B; Jackstadt, René; Herbrand, Ulrike; Schulenborg, Thomas; Marcus, Katrin; Klein-Scory, Susanne; Schmiegel, Wolff

2011-01-01

Inactivating mutations of SMAD4 are frequent in metastatic colorectal carcinomas. In previous analyses, we were able to show that restoration of Smad4 expression in Smad4-deficient SW480 human colon carcinoma cells was adequate to suppress tumorigenicity and invasive potential, whereas in vitro cell growth was not affected. Using this cellular model system, we searched for new Smad4 targets comparing nuclear subproteomes derived from Smad4 re-expressing and Smad4 negative SW480 cells. High resolution two-dimensional (2D) gel electrophoresis was applied to identify novel Smad4 targets in the nuclear subproteome of Smad4 re-expressing SW480 cells. The identified candidate protein Keratin 23 was further characterized by tandem affinity purification. Immunoprecipitation, subfractionation and immunolocalization studies in combination with RNAi were used to validate the Keratin 23-14-3-3ε interaction. We identified keratins 8 and 18, heat shock proteins 60 and 70, plectin 1, as well as 14-3-3ε and γ as novel proteins present in the KRT23-interacting complex. Co-immunoprecipitation and subfractionation analyses as well as immunolocalization studies in our Smad4-SW480 model cells provided further evidence that KRT23 associates with 14-3-3ε and that Smad4 dependent KRT23 up-regulation induces a shift of the 14-3-3ε protein from a nuclear to a cytoplasmic localization. Based on our findings we propose a new regulatory circuitry involving Smad4 dependent up-regulation of KRT23 (directly or indirectly) which in turn modulates the interaction between KRT23 and 14-3-3ε leading to a cytoplasmic sequestration of 14-3-3ε. This cytoplasmic KRT23-14-3-3 interaction may alter the functional status of the well described 14-3-3 scaffold protein, known to regulate key cellular processes, such as signal transduction, cell cycle control, and apoptosis and may thus be a previously unappreciated facet of the Smad4 tumor suppressive circuitry

Põneva kappkemmerguga mõisahoones hakkab sõitma lift / Vilja Kohler

Index Scriptorium Estoniae

Kohler, Vilja, 1966-

2005-01-01

Tartumaal asuva Tammistu mõisa rekonstrueerimisest, projekt AS Restor arhitektidelt Mart Keskkülalt ja Reet Saluverelt. Peahoonesse tuleb puuetega laste rehabilitatsioonikeskus. Kommenteerib Meelis Joost

Valitsuskabinet teeb ettepaneku kinnitada Eesti Raudtee erastajaks BRS

Index Scriptorium Estoniae

2004-01-01

Majandus- ja kommunikatsiooniminister Meelis Atonen tutvustas valitsuskabineti liikmetele AS-i Eesti Raudtee erastamise otsuse eelnõu, millega tehakse ettepanek kinnitada parimaks pakkujaks Baltic Rail Service

Transcriptional analysis and molecular dynamics simulations reveal the mechanism of toxic metals removal and efflux pumps in Lysinibacillus sphaericus OT4b.31

KAUST Repository

Shaw, Dario Rangel

2017-11-23

Lysinibacillus sphaericus strain OT4b.31 is a bacterium widely applied in bioremediation processes of hydrocarbon and metal polluted environments. In this study, we identified the molecular mechanism underlying the Pb2+ and Cr6+ resistance. Metal uptake and temporal transcription patterns of metal resistance operons were evaluated using reverse-transcribed quantitative PCR amplification. The function of the resistance determinants was studied applying docking and in silico mutagenesis methods. The results revealed that the adaptation of Lysinibacillus sphaericus OT4b.31 to elevated levels of lead and chromium involves the pbr and chr operons which comprise a transcriptional regulatory component (pbrR and chrB) and efflux ATPases (pbrA and chrA) to expel ions from the cytoplasm. Expression of metal resistance genes was constitutive and specifically inducible to the exposure of Pb2+ and Cr6+. The simultaneous presence of cations didn\\'t affect the bioaccumulation of metals, evidencing the multimetal resistance of L. sphaericus. Docking analysis revealed the key metal-protein interactions and the conformational changes after metal or ATP binding. Results showed that residues with aromatic rings or imidazole in the catalytic domain are crucial for metal binding and achievement of the function. To our knowledge, this is the first report of a specific mechanism for lead and chromium resistance in Lysinibacillus genus. From the findings of this study, it is possible to suggest the bacterium as a suitable candidate for rapid toxic metals bioremediation processes.

Infotehnoloogiateenistus / Meelis Kukk

Index Scriptorium Estoniae

Kukk, Meelis

2004-01-01

Ilmunud ka: 2003 : annual report : [Eesti Raudtee]. - Tallinn, 2004, lk. 36; 2003 : godovoi ottshjot : [Eesti Raudtee]. - Tallinn, 2004, lk. 36. Ülevaade AS-i Eesti Raudtee infotehnoloogiteenistuse tegvusest 2003. aastal

Pankade vastulöök kaardikopeerijaile / Mariliis Pinn

Index Scriptorium Estoniae

Pinn, Mariliis

2008-01-01

Sampo Pank ja Nordea Pank paigaldavad sularahaautomaatidele antiskimmingu ehk kaardi magnetribalt kaardiandmete kopeerimise ja PIN-koodi varastamise vastased seadmed. Vt samas: Näiteid elust. Kommenteerib Meelis Nurk

Neiud meenutavad küüditamise aegu / Raimu Hanson

Index Scriptorium Estoniae

Hanson, Raimu, 1957-

2006-01-01

Vilde Teatri noortestuudio esietendab 12. apr. Vilde Teatri keldrisaalis lavastust "Rong see...". Mängivad noored teatrihuvilised Karlova gümnaasiumist ja Reiniku Gümnaasiumist, lavastaja Meelis Hansing

Identification of epigenetically regulated genes that predict patient outcome in neuroblastoma

International Nuclear Information System (INIS)

Carén, Helena; Djos, Anna; Nethander, Maria; Sjöberg, Rose-Marie; Kogner, Per; Enström, Camilla; Nilsson, Staffan; Martinsson, Tommy

2011-01-01

Epigenetic mechanisms such as DNA methylation and histone modifications are important regulators of gene expression and are frequently involved in silencing tumor suppressor genes. In order to identify genes that are epigenetically regulated in neuroblastoma tumors, we treated four neuroblastoma cell lines with the demethylating agent 5-Aza-2'-deoxycytidine (5-Aza-dC) either separately or in conjunction with the histone deacetylase inhibitor trichostatin A (TSA). Expression was analyzed using whole-genome expression arrays to identify genes activated by the treatment. These data were then combined with data from genome-wide DNA methylation arrays to identify candidate genes silenced in neuroblastoma due to DNA methylation. We present eight genes (KRT19, PRKCDBP, SCNN1A, POU2F2, TGFBI, COL1A2, DHRS3 and DUSP23) that are methylated in neuroblastoma, most of them not previously reported as such, some of which also distinguish between biological subsets of neuroblastoma tumors. Differential methylation was observed for the genes SCNN1A (p < 0.001), PRKCDBP (p < 0.001) and KRT19 (p < 0.01). Among these, the mRNA expression of KRT19 and PRKCDBP was significantly lower in patients that have died from the disease compared with patients with no evidence of disease (fold change -8.3, p = 0.01 for KRT19 and fold change -2.4, p = 0.04 for PRKCDBP). In our study, a low methylation frequency of SCNN1A, PRKCDBP and KRT19 is significantly associated with favorable outcome in neuroblastoma. It is likely that analysis of specific DNA methylation will be one of several methods in future patient therapy stratification protocols for treatment of childhood neuroblastomas

Effect of Ozone Therapy (OT on Healing of Colonic Anastomosis in a Rat Model of Peritonitis

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Başak Erginel

2014-09-01

Full Text Available Background: Ozone is a three-oxygen molecule (O3. Ozone therapy (OT is systematically effective when pathological inflammatory and immunologic processes are activated. Among of these conditions are wound healing, macular degeneration related to aging, and conditions that are ischemic or infectious. Aims: The aim of this study was to determine the effects of OT on wound healing of intestinal anastomosis in the presence of peritonitis in a rat model. Study Design: Animal experimentation. Methods: A total of 40 Wistar albino rats were randomized into four groups (n=10 including: sham (S, peritonitis (P, ozone 0 (O0, and ozone 24 (O24. In group S, only cecal dissection was carried out. The S group had only a cecal dissection and intestinal anastomosis performed, but no peritonitis. In all other groups, cecal ligation and puncture (CLP followed the cecal dissection to induce bacterial peritonitis. 24 h after puncture, a cecal resection and ileocolic anastomosis were performed. In group P, 24 h after CLP, a cecal resection and ileocolic anastomosis were performed and no ozone was administered. In group O0, immediately after the anastomosis, and in group O24, starting 24 hours after the anastomosis, an intraperitoneal 1 mg/kg/day ozone administration was applied for seven days. On the seventh day the animals were sacrificed, the anastomotic bursting pressures (BP and the hydroxyproline values of the anastomotic tissues were measured, and histopathologic examination of the anastomotic segment was carried out. Results: The highest BP was in group S, with 211±23.13 mmHg. The mean BP of group P was 141±56.25 mmHg, which was significantly lower than in the other two peritonitis groups that received ozone therapy, group O0 and O24, where it was 192±22 and 166±45 mmHg, respectively (p0.05. Histopathologic analyses of the anastomotic segments determined there was significantly more oedema and necrosis in the control group rats, and collagen deposition in

Baltic Property Trust - professionaalseim kinnisvara investeerimisfirma Baltimere piirkonnas

Index Scriptorium Estoniae

2005-01-01

BPT Eesti direktor Meelis Liivak Baltic Property Trusti (BPT) eesmärkidest, tegevusvaldkondadest ja tulevikuplaanidest. Kommenteerivad Alo Kullamaa, Priit Põldoja, Andres Järving ja Aivar Tomson. Lisa: Ajalugu

DNA methylation patterns of imprinting centers for H19, SNRPN, and KCNQ1OT1 in single-cell clones of human amniotic fluid mesenchymal stem cell

Directory of Open Access Journals (Sweden)

Hsiu-Huei Peng

2012-09-01

Conclusion: In conclusion, human amniotic fluid mesenchymal stem cells contain a unique epigenetic signature during in vitro cell culture. H19 and KCNQ1OT1 possessed a substantial degree of hypermethylation status, and variable DNA methylation patterns of SNRPN was observed during in vitro cell culture of human amniotic fluid mesenchymal stem cells. Our results urge further understanding of epigenetic status of human amniotic fluid mesenchymal stem cells before it is applied in cell replacement therapy.

Rae vallajuhte kahtlustatakse korruptsioonis / Kärt Anvelt

Index Scriptorium Estoniae

Anvelt, Kärt, 1973-

2010-01-01

Rae vallavanemat Veigo Gutmanni ja abivallavanemat Meelis Kasemaad kahtlustatakse selles, et osaledes Lehmja külas asuvate kinnistute detailplaneeringutega seotud otsustusprotsessides, varjasid nad oma varalisi huve seoses nimetatud kinnistutega

Tallinna kesklinna sissesõidu võiks maksustada / Brigitte Ollier, Hans Rat ; interv. Toivo Tänavsuu

Index Scriptorium Estoniae

Ollier, Brigitte

2004-01-01

Tallinnas viibinud Ülemaailmse Ühistranspordi Assotsiatsiooni (UITP) peasekretär ja direktor vastavad küsimustele, mis on seotud Tallinna ühistranspordiga. Kommenteerivad Toomas Vitsut ja Meelis Atonen

Kambakas Taistole / Janar Filippov

Index Scriptorium Estoniae

Filippov, Janar, 1976-

2003-01-01

Autoettevõtete Liit ning 19 bussifirma juhti esitasid siseminister Margus Leivole ning majandus- ja kommunikatsiooniminister Meelis Atonenile pöördumise bussiettevõtte Taisto kohta. Küsimustele vastab Tajo Saluste

Kesklinna politseihoone = The Central Tallinn Police Department / Margit Mutso

Index Scriptorium Estoniae

Mutso, Margit, 1966-

2010-01-01

Tallinnas Kolde pst. 65 asuva Kesklinna politseihoone arhitektuursest lahendusest. Arhitekt, sisearhitekt Meeli Truu, kaasautor Gersti Siimon (AS Nord Projekt). Žürii liikme Kalle Komissarovi hinnang kultuurkapitali aastapreemiale esitatud hoonele

Melodija tranzita / Ljubov Semjonova

Index Scriptorium Estoniae

Semjonova, Ljubov

2004-01-01

Laeval Meloodia toimunud rahvusvahelise transiidi teemalisest konverentsist, millest teiste hulgas võtsid osa Eesti endine majandus- ja kommunikatsiooniminister Meelis Atonen ning Soome transpordi- ja tööstusminister Mauri Pekkarinen

Kuulujututurunduse abil Eestis veel müüa ei õnnestu / Katrin Rohtla

Index Scriptorium Estoniae

Rohtla, Katrin, 1966-

2007-01-01

Maailmas on üha enam levimas kuulujututurundus (buzzmarketing), kus ettevõtted annavad trenditeadlikele arvamusliidritele testimiseks tooteid lootuses, et nood tooteid oma tutvusringkonnale edasi tutvustaksid. Kommenteerivad Merit Raju ja Meelis Vill

Microsoft võttis Eesti koostööpakkumise vastu / Kristjan Otsmann

Index Scriptorium Estoniae

Otsmann, Kristjan, 1971-

2004-01-01

Ida-Euroopa riikide ja Microsofti ümarlaud Slovakkias Bratislavas. Microsofti asutaja Bill Gates oli huvitatud Meelis Atoneni ettepanekust, et riik korraldab oma kodanikele Microsofti tarkvara rentimise neile vastuvõetava hinnaga

"Hair" - laul ja tants, mitte kultusmuusikal / Andris Feldmanis

Index Scriptorium Estoniae

Feldmanis, Andris, 1982-

2006-01-01

Kultusmuusikal "Hair" Rock Cafés Georg Otsa nim. Tallinna Muusikakooli õpilaste esituses, lavastaja Ivo Eensalu, muusikajuhid Mare Väljataga, Meelis Punder, tantsujuht Ulrike Ustav, kunstnikud Kristel Viks, Liisi Suuroja

MULTISEASONAL TREE CROWN STRUCTURE MAPPING WITH POINT CLOUDS FROM OTS QUADROCOPTER SYSTEMS

Directory of Open Access Journals (Sweden)

S. Hese

2017-08-01

Full Text Available OTF (Off The Shelf quadro copter systems provide a cost effective (below 2000 Euro, flexible and mobile platform for high resolution point cloud mapping. Various studies showed the full potential of these small and flexible platforms. Especially in very tight and complex 3D environments the automatic obstacle avoidance, low copter weight, long flight times and precise maneuvering are important advantages of these small OTS systems in comparison with larger octocopter systems. This study examines the potential of the DJI Phantom 4 pro series and the Phantom 3A series for within-stand and forest tree crown 3D point cloud mapping using both within stand oblique imaging in different altitude levels and data captured from a nadir perspective. On a test site in Brandenburg/Germany a beach crown was selected and measured with 3 different altitude levels in Point Of Interest (POI mode with oblique data capturing and deriving one nadir mosaic created with 85/85 % overlap using Drone Deploy automatic mapping software. Three different flight campaigns were performed, one in September 2016 (leaf-on, one in March 2017 (leaf-off and one in May 2017 (leaf-on to derive point clouds from different crown structure and phenological situations – covering the leaf-on and leafoff status of the tree crown. After height correction, the point clouds where used with GPS geo referencing to calculate voxel based densities on 50 × 10 × 10 cm voxel definitions using a topological network of chessboard image objects in 0,5 m height steps in an object based image processing environment. Comparison between leaf-off and leaf-on status was done on volume pixel definitions comparing the attributed point densities per volume and plotting the resulting values as a function of distance to the crown center. In the leaf-off status SFM (structure from motion algorithms clearly identified the central stem and also secondary branch systems. While the penetration into the

Multiseasonal Tree Crown Structure Mapping with Point Clouds from OTS Quadrocopter Systems

Science.gov (United States)

Hese, S.; Behrendt, F.

2017-08-01

OTF (Off The Shelf) quadro copter systems provide a cost effective (below 2000 Euro), flexible and mobile platform for high resolution point cloud mapping. Various studies showed the full potential of these small and flexible platforms. Especially in very tight and complex 3D environments the automatic obstacle avoidance, low copter weight, long flight times and precise maneuvering are important advantages of these small OTS systems in comparison with larger octocopter systems. This study examines the potential of the DJI Phantom 4 pro series and the Phantom 3A series for within-stand and forest tree crown 3D point cloud mapping using both within stand oblique imaging in different altitude levels and data captured from a nadir perspective. On a test site in Brandenburg/Germany a beach crown was selected and measured with 3 different altitude levels in Point Of Interest (POI) mode with oblique data capturing and deriving one nadir mosaic created with 85/85 % overlap using Drone Deploy automatic mapping software. Three different flight campaigns were performed, one in September 2016 (leaf-on), one in March 2017 (leaf-off) and one in May 2017 (leaf-on) to derive point clouds from different crown structure and phenological situations - covering the leaf-on and leafoff status of the tree crown. After height correction, the point clouds where used with GPS geo referencing to calculate voxel based densities on 50 × 10 × 10 cm voxel definitions using a topological network of chessboard image objects in 0,5 m height steps in an object based image processing environment. Comparison between leaf-off and leaf-on status was done on volume pixel definitions comparing the attributed point densities per volume and plotting the resulting values as a function of distance to the crown center. In the leaf-off status SFM (structure from motion) algorithms clearly identified the central stem and also secondary branch systems. While the penetration into the crown

Roostevabast terasest pind, kuhu ei jää sõrmejälgi

Index Scriptorium Estoniae

2007-01-01

Tootejuht Meelis Paas tutvustab Saksa firma Busch-Jaeger uut roostevabast terasest elektritarvikute sarja pure, kuhu kuulub 80 erinevat toodet. Sarja pure lülitid-pistikupesad sobivad ideaalselt kööki

Eesti animafilm osales Annecys

Index Scriptorium Estoniae

2004-01-01

Animafilmifestivalil Annecys osales neli Eesti filmi : Priit Pärna joonisfilm "Karl ja Marilyn", Rao Heidmetsa nukufilm "Instinkt", David Snowmani joonisfilm "Frank ja Wendy. Hungerburger" ja Meelis Arulepa reklaamfilm "Animated Dreams 2003"

Telepoisi kolmas tulemine / Jaanus Kulli

Index Scriptorium Estoniae

Kulli, Jaanus, 1955-

2008-01-01

ETV lastesaadete esimese telepoisi looja oli 1960. aastatel Jaak Palmse, 2000. aastatel "taastas" telepoisi Meelis Arulepp ja 2008.a. ilmus telepoiss ekraanile Daniel Müntineni ja animatsioonistuudio Birds of Paradise loominguna

Eurovisiooni vaheklipp murrab teed animailmas / Neeme Korv

Index Scriptorium Estoniae

Korv, Neeme, 1974-

2003-01-01

Mulluse Eurovisiooni lauluvõistluse Tallinna kontserdi üks vaheklippidest "Saabastega kass", mille režissöörid on René Vilbre ja Meelis Arulepp, valiti maineka Annecy animafilmifestivali nominentide hulka

Nightlife duo Meri ja Julm / Siim Nestor

Index Scriptorium Estoniae

Nestor, Siim, 1974-

2002-01-01

Remikside produktsiooni-duost Rulers of the Deep (ROTD), koosseisus Meelis Meri ja P. Julm (Priit Juurman), firma Defected singli ATFC featuring Lisa Milleett "Sleep Talk" edust inglise edetabelites, millel M. Meri remiksid

Skype has changed the telecommunications market / Lauri Levo

Index Scriptorium Estoniae

Levo, Lauri

2005-01-01

Eestlaste kaasabil loodud programmist Skype, millega saab Interneti vahendusel teha tasuta telefonikõnesid üle maailma. Meelis Kuusbergi kommentaar. Lisad: Ajalugu; Niklas Zennström; Janus Friis. Vt. samas intervjuud Sten Tamkiviga

Ministry prepares to buy back rail company / Kairi Kurm

Index Scriptorium Estoniae

Kurm, Kairi

2006-01-01

Eesti valitsuse teatel on tehtud ettepanek osta tagasi 66 protsenti Eesti Raudtee osalusest. Omaniku Edward Burkhardt'i ja Eesti poliitikute seisukohad. Kommenteerivad: Agu Uudelepp, Toomas Raag, Meelis Atonen, Margus Tsahkna, Eiki Nestor

Tri gorsti za Tsentralnõi rõnok / Arvi Tapver

Index Scriptorium Estoniae

Tapver, Arvi

1999-01-01

Mõrvatud endise abilinnapea Mait Metsamaa keskturu erastajatest kaaskond kahlustas tunnistaja sõnul topeltmängu, kui Metsamaa vanale sõbrale ja allilmas kardetavale Meelis Laole erastamisest loobumise eest hüvitist pakkus

Eduard Odinets : "Olen eestlane, kelle emakeel on vene keel" / Eduard Odinets ; interv. Erik Kalda

Index Scriptorium Estoniae

Odinets, Eduard, 1976-

2006-01-01

Ilmunud ka: Severnoje Poberezhje Ilmunud ka: Severnoje Poberezhje : Subbota 3. juuni lk. 3. Riigikogu sotsiaaldemokraatide fraktsiooni nõunik oma poliitikukarjäärist. Arvamust avaldab Katrin Saks ja Meelis Goldberg (Severnoje Pob.)

Anneli Remme soovitab : Kammerdžässi kava "Jõulud mõisas" / Siim Nestor

Index Scriptorium Estoniae

Nestor, Siim, 1974-

2006-01-01

Eesti-Tšiili trio Raivo Tafenau - Meelis Vind - Ricardo Padilla kontsertidest sarja "Mõisaromantika" raames 25. nov. Taagepera lossis Valgamaal, 26. nov. Tõstamaa mõisas Pärnumaal ja 16. dets. Vihterpalu mõisas Harjumaal

Eestikeelse loomuliku kõnesünteesi poole / Diana Krull

Index Scriptorium Estoniae

Krull, Diana, 1930-2013

2008-01-01

Arvustus: Mihkla, Meelis. Kõne ajalise struktuuri modelleerimine eestikeelsele tekst-kõne sünteesile = Modelling the temporal stucture of speech for the Estonian text-to-speech synthesis. Tartu : Tartu Ülikooli Kirjastus, 2007

Purskkaev toas - närv puhanud ja õhk niiske / Signe Kalberg

Index Scriptorium Estoniae

Kalberg, Signe, 1959-

2010-01-01

Õhuniiskuse säilitamiseks, liiklusmüra summutamiseks või meeli rahustava veevulina kuulamiseks võib tuppa tuua purskkaevu. Tubaste purskkaevude valikust. Geobioloog ja feng shui spetsialist Siret Seeder purskkaevu asukohast kodus

A Mouse Model of Hyperproliferative Human Epithelium Validated by Keratin Profiling Shows an Aberrant Cytoskeletal Response to Injury

Directory of Open Access Journals (Sweden)

Samal Zhussupbekova

2016-07-01

Full Text Available A validated animal model would assist with research on the immunological consequences of the chronic expression of stress keratins KRT6, KRT16, and KRT17, as observed in human pre-malignant hyperproliferative epithelium. Here we examine keratin gene expression profile in skin from mice expressing the E7 oncoprotein of HPV16 (K14E7 demonstrating persistently hyperproliferative epithelium, in nontransgenic mouse skin, and in hyperproliferative actinic keratosis lesions from human skin. We demonstrate that K14E7 mouse skin overexpresses stress keratins in a similar manner to human actinic keratoses, that overexpression is a consequence of epithelial hyperproliferation induced by E7, and that overexpression further increases in response to injury. As stress keratins modify local immunity and epithelial cell function and differentiation, the K14E7 mouse model should permit study of how continued overexpression of stress keratins impacts on epithelial tumor development and on local innate and adaptive immunity.

Restoran Cha Dao = Restaurant Cha Dao

Index Scriptorium Estoniae

2012-01-01

Tallinnas Suur-Patarei 2 asuva Hiina restorani Cha Dao sisekujundusest. Restorani mööbel, v.a toolid, projekteeriti eritellimusena. Sisearhitekt Dmitri Pisarenko (DM3 OÜ), loetletud tema töid. Arhitekt Meeli Truu

Kolhoosikeskus - oma ja võõras = Collective farm centre - familiar and alien / Liina Jänes ; tõlk. Liisi Ojamaa

Index Scriptorium Estoniae

Jänes, Liina, 1977-

2000-01-01

Eesti kolhoosikeskuste arhitektuurist. Vaadeldakse aastail 1957-1990 ehitatud 24 keskusehoonet. Arhitektid Nikolai Kusmin, Harri Kingo, Toomas Rein, Vilen Künnapu, Ain Padrik, Maara Metsal, Meeli Truu jt. 17 välisvaadet, 3 joon., kaart

Kuidas möödus 2012. aasta komplekteerimises? / Külli Karu, Rutt Enok, Kaili Õunapuu-Seidelberg ... [jt.

Index Scriptorium Estoniae

2013-01-01

Vastavad Külli Karu Tallinna Keskraamatukogust, Rutt Enok Harju Maakonnaraamatukogust, Kaili Õunapuu-Seidelberg Lääne-Virumaa Keskraamatukogust, Tiina Talvet Rapla Keskraamatukogust, Toivo Ärtis Kambja raamatukogust ning kultuuriministeeriumi raamatukogunõunik Meeli Veskus

Lozhitsja na relsõ poka smõsla net / Niina Antonova

Index Scriptorium Estoniae

Antonova, Niina

2004-01-01

Peaministri ülesannetes majandus- ja kommunikatsiooniministri Meelis Atoneni pressikonverentsist Narva-visiidi tulemustest. Parlamendiliige Mihhail Stalnuhhin ja Eesti Raudtee juhatuse liige rääkisid Narva raudteejaama rekonstrueerimise projektist ning soojuse uue hinna kooskõlastamisest

Kassikullatud mausoleum kadunud aja ideoloogiale / Johannes Saar

Index Scriptorium Estoniae

Saar, Johannes, 1965-

2004-01-01

Ilja ja Emilia Kabakovi ning Raul Meel'i ühisnäitus Tallinna Kunstihoones. Kabakovid on toonud Kunstihoone suurde saali korduse 1993. a. Frankfurdis esmaesitatud installatsiooniideest "Tühi muuseum". Totaalse installatsiooni meistrist Ilja Kabakovist

Co-option of Hair Follicle Keratins into Amelogenesis Is Associated with the Evolution of Prismatic Enamel: A Hypothesis

Directory of Open Access Journals (Sweden)

Elia Beniash

2017-10-01

Full Text Available Recent discovery of hair follicle keratin 75 (KRT75 in enamel raises questions about the function of this protein in enamel and the mechanisms of its secretion. It is also not clear how this protein with a very specific and narrow expression pattern, limited to the inner root sheath of the hair follicle, became associated with enamel. We propose a hypothesis that KRT75 was co-opted by ameloblasts during the evolution of Tomes' process and the prismatic enamel in synapsids.

24. IV avati Tartu Kunstimajas...

Index Scriptorium Estoniae

2003-01-01

Vallo Kalamehe ja Peeter Linnapi kureeritud fotonäitus "XXL. Eesti fotoklassika". Osalevad Herkki-Erich Merila, Tarvo Hanno Varres, Peeter Laurits, Toomas Kalve, P. Linnap, V. Kalamees, Malev Toom, Madis Palm, Tõnu Tamm, Meelis Lokk

Disainkaminad

Index Scriptorium Estoniae

2007-01-01

Salongides Tulering, Tulepesad ja 12 kaminat ning Tondi kaminasalongis pakutavad kaminad. Kaminad Liina Ainomäe, Annes Arro, Kaire Kemp-Tischleri, Jorma Holmi, Külli Salumi ja Riina Hariku sisekujundusega ning Meelis Pressi prpjekteeritud eramutes

Kriisikoldeid kustutab (teatri)mäng / Rait Avestik

Index Scriptorium Estoniae

Avestik, Rait, 1974-

2010-01-01

Itaallaste Nino d’Introna ja Giacomo Ravicchio teksti põhjal valminud seiklusloost „Robinson & Crusoe”, tõlkinud Kirke Org, lavastanud Aare Toikka. Esietendus 27. nov. 2009 VAT-teatris. Mängivad Meelis Põdersoo ja Ago Soots

Puls Breweryl on vurts väljas / Asso Puidet

Index Scriptorium Estoniae

Puidet, Asso, 1977-

2008-01-01

AS Puls Brewery tegevjuht Meelis Pihel tunnistab, et loogiliseks sammuks oleks ettevõttel liituda mõne suurema korporatsiooniga. Diagramm: Pärnu õlletootmise tõusud ja mõõnad. Lisa: Ütlemised läbi aastatel

Pressure effects on enzyme reactions in mainly organic media: alpha-chymotrypsin in reversed micelles of Aerosol OT in octane.

Science.gov (United States)

Mozhaev, V V; Bec, N; Balny, C

1994-08-01

Biocatalytic transformations in reversed micelles formed by anionic surfactant Aerosol OT in octane have been studied at high pressures by an example of alpha-chymotrypsin-catalyzed hydrolysis of N-carbobenzoxy-L-tyrosine p-nitrophenyl ester and N-succinyl-L-phenylalanine p-nitroanilide. For the first time it has been found that the enzyme retains high activity in these water-in-oil microemulsions up to a pressure of 2 kbar. The value of the activation volume (delta V*) for the enzyme reactions shows a dependence on the water content in the system. When the size of the micellar aqueous inner cavity (as evaluated at 1 atm) approaches the molecular size of alpha-chymotrypsin, delta V* becomes significantly different from the value in aqueous solution and in the micelles with a larger size. Possibilities of regulating the enzyme activity by pressure in systems with a low content of water are discussed.

Dinámica de construcción territorial en los asentamientos del río Otún en Pereira (1950- 2000: fenomenología a la territorialidad del sujeto habitante

Directory of Open Access Journals (Sweden)

Julio César Murillo García

2016-06-01

Full Text Available El presente artículo surge como resultado del análisis- interpretación a la dinámica territorial en los asentamientos informales ubicados sobre las márgenes del río Otún a su paso por la comuna Otún en la ciudad de Pereira, desde dos momentos nodales de su construcción: momento fundacional (1950 y momento de toma y apropiación de predios (2000. Como metodología se asume la fenomenología a la territorialidad cotidiana del habitante, explícitamente desde dos variables: contexto socioeconómico y vínculos territoriales. A partir de allí se consolidaron universos de análisis e interpretación sobre la territorialidad del sujeto que dan cuenta de fricciones y/o conflictos espaciales con respecto a la política pública territorial del municipio de Pereira, en medio de la ejecución de programas y proyectos de gestión y planificación territorial con un denotado carácter hegemónico. Con ello se despeja un panorama crítico reflexivo alrededor de la implementación de estas políticas y al desarrollo sostenible territorial.

Abilinnapea Ratas pidas uputust paratamatuseks / Jüri Ratas ; interv. Piret Reiljan

Index Scriptorium Estoniae

Ratas, Jüri, 1978-

2004-01-01

Tallinna abilinnapea Jüri Ratas kommenteerib olukorda linnas, kus kahe päevaga sadas maha rohkem kui juulikuu sademete norm ette näeb. Vt. samas Meelis Eldermann. Miks veevärk vihmaga toime ei tulnud?

Estlink sea cable project to proceed / Aleksei Gunter

Index Scriptorium Estoniae

Gunter, Aleksei, 1979-

2003-01-01

29. augustil kohtusid Tallinnas Soome kaubandus- ja tööstusminister Mauri Pekkarinen ja Eesti Vabariigi majandusminister Meelis Atonen, et välja töötada Soome-Eesti merealuse kaabli Estlink'i projekti üksikasjad

Mehelikkuse kriis / Margit Tõnson

Index Scriptorium Estoniae

Tõnson, Margit, 1978-

2009-01-01

Eesti uuemaid lühimängufilme : Sutharsan Bala "Krantsid" (operaator Meelis Veermets, produtsent Marianne Ostrat, osades Jekaterina Novosjolova, Paulo Kulešov ja Mihkel Ulki "Pihv" (stsenarist Triin Sinissaar, osades Mari Abel, Andres Mähar)

Soojalt sojast / Ülle Hõbemägi

Index Scriptorium Estoniae

Hõbemägi, Ülle

2008-01-01

Sojast, sojatoodetest ning sojatoitudest räägivad aroomterapeut ja massöör Marge Teder, Soya Foods Baltic OÜ müügidirektor Meeli Matsalu, tudeng ja taimetoitlane Loore-Emilie Raav ning Angelica Udeküll

"Cremaster' tsükkel" : Matthew Barney avastusretk kunsti ja filmikunsti vahealal / Miriam Dagan ; tõlk. Epp Aareleid

Index Scriptorium Estoniae

Dagan, Miriam

2007-01-01

Ameerika kunstniku ja režissööri kunstnikufilmidest "Cremaster' tsükkel" (1994-2002). Viie tsükli põhisündmusest. Viienda filmi rekvisiitor oli Meeli Salumäe, Kostabi Maailma assistent New Yorgis

Stiilne avarus / Aita Kivi

Index Scriptorium Estoniae

Kivi, Aita, 1954-

2004-01-01

Angelika ja Meelis Maalmanni perekonna korter (93 mø) Rannamõisa teel asuvas ridaelamus. Uue plaanilahenduse ja sisekujunduse autor Aet Piel. Ill.: 8 vaadet, korteri plaan enne ja nüüd. A. ja M. Maalmanni kommentaarid

Eesti reklaamide best of... / Andres Maimik

Index Scriptorium Estoniae

Maimik, Andres, 1970-

2008-01-01

Eesti reklaamikampaaniad: 1. http//www.assapauk.ee/. Mina olen pedofiil; 2. Vichy vesi. Mullnaised; 3. A le Coq Special. Usalda oma meeli; 4. SMS-laen. Kvaliteetinimene tarbib kvaliteetteenust; 5. Värska vurts. Preester ja koolitüdruk

Q Vara juhtimiskoolitus "Kuidas karastus teras" / Annika Vaikla, Keiu Talve

Index Scriptorium Estoniae

Vaikla, Annika

2007-01-01

Kinnisvarafirma OÜ Q Vara töötajad pidid läbi viima Paide rahvusvahelise triatloni, mis oli läbiviijatele juhtimiskoolituseks. Küsimusele, kas juhtkonnal tekkis kiusatus sekkuda, vastab Q Vara juhatuse esimees Meelis Šokmann

Lõplike vastusteta kunstnikeportreed / Riin Kübarsepp

Index Scriptorium Estoniae

Kübarsepp, Riin, 1978-

2004-01-01

Dokumentaalfilmid kunstnikest - Kert Grünbergi "Uputus" fotograaf Peeter Lauritsast (Multi Kultuurimaja 2003), Marianne Kõrveri "Pildi sisse minek" kostüümikunstnik Agu Pildist (Exitfilm 2002) ja Meelis Salujärve "Erki Kasemets" (Rühm+0 2002)

Eesti Ajaloomuuseumi väliekspositsioon "Klooga laager ja holokaust" = Outdoor exhibition of the Estonian History Museum "Klooga camp and the holocaust"

Index Scriptorium Estoniae

2015-01-01

Eesti Ajaloomuuseumi väliekspositsioon "Klooga laager ja holokaust" Kloogal, valminud 2013. Arhitektuuri sihtkapitali maastikuarhitektuuripreemia 2013. Arhitektuurne idee ja projekteerimine Mari Rass, Diana Taalfeld ja EA Reng. Kuraator Olev Liivik, konsultandid Ruth Bettina Birn, Tõnis Liibek, Meelis Maripuu

Pühapäev kunsti ja muusikaga / Mari Hiiemäe

Index Scriptorium Estoniae

Hiiemäe, Mari

2008-01-01

Jazzkaare 20. aprillil korraldatavast kunsti ja muusika pühapäevast Niguliste muuseumis (esineb East-West Quartet), Tallinna Kunstihoones (Estonian Guitar Octet), Rotermanni soolalaos (Raivo Tafenau ja Meelis Vindi duo) ja Kumu auditooriumis (laulja Kelli Uustani plaadiesitlus)

Eesti animafilm võitis UNICEFi auhinna

Index Scriptorium Estoniae

2007-01-01

A Film Eesti lastelühianimafilm (režissöörid Aina Järvine ja Meelis Arulepp) tunnistati parimaks haridus- ja sotsiaalteemaliseks filmiks 19.-22. aprillini Itaalias toimunud "Cartoons on the Bay" festivalil ja sai Pulcinella auhinna

CD80 and CD86 Costimulatory Molecules Differentially Regulate OT-II CD4+ T Lymphocyte Proliferation and Cytokine Response in Cocultures with Antigen-Presenting Cells Derived from Pregnant and Pseudopregnant Mice

Directory of Open Access Journals (Sweden)

Tomasz Maj

2014-01-01

Full Text Available Immune phenomena during the preimplantation period of pregnancy are poorly understood. The aim of our study was to assess the capacity for antigen presentation of splenic antigen-presenting cells (APCs derived from pregnant and pseudopregnant mice in in vitro conditions. Therefore, sorted CD11c+ dendritic cells and macrophages F4/80+ and CD11b+ presenting ovalbumin (OVA were cocultured with CD4+ T cells derived from OT-II mice’s (C57BL6/J-Tg(TcraTcrb1100Mjb/J spleen. After 132 hours of cell culture, proliferation of lymphocytes (ELISA-BrdU, activation of these cells (flow cytometry, cytokine profile (ELISA, and influence of costimulatory molecules blocking on these parameters were measured. We did not detect any differences in regulation of Th1/Th2 cytokine balance. CD86 seems to be the main costimulatory molecule involved in the proliferation response but CD80 is the main costimulatory molecule influencing cytokine secretion in pregnant mice. In conclusion, this study showed that CD80 and CD86 costimulatory molecules regulate OT-II CD4+ T lymphocyte proliferation and cytokine response in cocultures with antigen-presenting cells derived from pregnant and pseudopregnant mice. The implications of these changes still remain unclear.

Suur fondijuhtide ülevaade

Index Scriptorium Estoniae

2006-01-01

Küsimustele vastavad Toomas Reisenbuk, Valdur Jaht, Mehis Raud, Siim Valner, Vahur Madisson, Meelis Angerma, Alo Kullamaa, Endriko Võrklaev, Arne Randmaa, Andrei Zaborski, Robert Kitt, Fabio Filipozzi, Martin Hendre, Piotr Chorzewski, Artjom Saia, Aari Stalde ja Märten Kress

"Magnus" lummas Riia vaatajaid / Kristi Leppik

Index Scriptorium Estoniae

Leppik, Kristi

2008-01-01

Kadri Kõusaare Cannes'i programmis esilinastunud debüütfilmi "Magnus" esilinastuselt Läti väärtfilmikinos Cinema Riga, kus kohal olid režissöör ning peaosatäitja Kristjan Kasearu. Lisatud Meelis Muhu "Arvamus"

Omanikud lõpetavad skandaalse firma Kuressaare Golf tegevuse / Andres Sepp

Index Scriptorium Estoniae

Sepp, Andres, 1976-

2004-01-01

AS Kuressaare Golf likvideeritakse, sest Põduste jõe äärde kavandatud golfiväljaku ehitamise plaanid sumbusid, kuna firma aktsionäride vahel tekitas tüli rahakantimise kahtlus. Kommenteerib AS-i Kuressaare Golf juhatuse liige Meelis Põlda

Atonen ja Aaviksoo tegid eile esimese 3G kõne / Viktoria Korpan

Index Scriptorium Estoniae

Korpan, Viktoria, 1976-

2003-01-01

Majandusminister Meelis Atonen ja Eesti Mobiiltelefoni juht Peep Aaviksoo pidasid EMT tehnoloogiakeskuses esimese ametliku kõne 3G testvõrgus. Lisaks tavakõnele videokõnet ja kiiret andmesidet võimaldav teenus jõuab kommertskasutusse orienteeruvalt aastal 2005, lubas Aaviksoo

Internet nagu postiteenus / Anneli Ammas

Index Scriptorium Estoniae

Ammas, Anneli, 1962-

2004-01-01

Majandus- ja kommunikatsiooniminister Meelis Atoneni sõnul peaks Interneti-teenus muutuma sarnaseks postiteenusega, millel on ühtne hind üle Eesti. Bill Gates on huvitatud Eesti ettepanekust pakkuda Eesti kodutarbijale tarkvara tervikpaketist vaid seda osa, mida tarbija tegelikult kasutab

Euroopluse origami / Meelis Oidsalu

Index Scriptorium Estoniae

Oidsalu, Meelis, 1978-

2011-01-01

17. septembril NO99 teatris esietendunud kolme teatri - NO99, Münchner Kammerspiele, Lyric Hammersmith Theatre - koostöös valminud lavastusest "Three Kingdoms" ("Kolm kuningriiki"), teksti autor Simon Stephens, lavastaja Sebastian Nübling

Multikultuurses Horvaatias / Meeli Veskus

Index Scriptorium Estoniae

Veskus, Meeli, 1953-

2005-01-01

NAPLE 2. konverents أRahvusraamatukogude roll rahvuslikes poliitikates suundumisel teadmistepõhise ühiskonna poole: kultuuripärandi digiteerimine ja mitmekultuurilisuse rakendamine raamatukogudesؤ peeti 5.-8. oktoobrini 2005 Supetaris Horvaatias

Turvaline tulevik / Meelis Piller

Index Scriptorium Estoniae

Piller, Meelis

2002-01-01

Arutlus kas turvalise tuleviku eelduseks on privaatsusest loobumine. Ka probleemi "turvalisus versus privaatsus" tõstatanud Robert Ludlumi romaanist "Prometheuse pettus" ja ideede käsitlusest Ray Bradbury ulmeteoses "451° Fahrenheiti". Näitena on toodud ka Steven Spielbergi filmi "Minority report"

Privaatsuse õigusest / Meelis Leis

Index Scriptorium Estoniae

Leis, Meelis

1998-01-01

Privaatsuse kui kaitstava hüve tekkeloost, selle õiguslikust ja psühholoogilisest käsitlusest, privaatsuse õigusest kohustisõiguses, Euroopa inimõiguste ja põhivabaduste kaitse konventsioonis ning Saksa, USA ja Eesti õiguskorras

Secondary Metabolite Profiling of Species of the Genus Usnea by UHPLC-ESI-OT-MS-MS

Directory of Open Access Journals (Sweden)

Francisco Salgado

2017-12-01

Full Text Available Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria, which are considered among the slowest growing organisms, with strong tolerance to adverse environmental conditions. There are about 400 genera and 1600 species of lichens and those belonging to the Usnea genus comprise about 360 of these species. Usnea lichens have been used since ancient times as dyes, cosmetics, preservatives, deodorants and folk medicines. The phytochemistry of the Usnea genus includes more than 60 compounds which belong to the following classes: depsides, depsidones, depsones, lactones, quinones, phenolics, polysaccharides, fatty acids and dibenzofurans. Due to scarce knowledge of metabolomic profiles of Usnea species (U. barbata, U. antarctica, U. rubicunda and U. subfloridana, a study based on UHPLC-ESI-OT-MS-MS was performed for a comprehensive characterization of their secondary metabolites. From the methanolic extracts of these species a total of 73 metabolites were identified for the first time using this hyphenated technique, including 34 compounds in U. barbata, 21 in U. antarctica, 38 in U. rubicunda and 37 in U. subfloridana. Besides, a total of 13 metabolites were not identified and reported so far, and could be new according to our data analysis. This study showed that this hyphenated technique is rapid, effective and accurate for phytochemical identification of lichen metabolites and the data collected could be useful for chemotaxonomic studies.

Vy jste mi z té otázky utekl (analýza jednoho způsobu vymáhání odpovědi s využitím korpusových dokladů)

Czech Academy of Sciences Publication Activity Database

Jílková, Lucie

2015-01-01

Roč. 97, č. 1 (2015), s. 31-11 ISSN 0008-7386 R&D Projects: GA ČR GAP406/12/1829 Institutional support: RVO:68378092 Keywords : the expression vy mi utíkáte z otázky * the SYN2013PUB corpus * the DIALOG corpus, metaphor Subject RIV: AI - Linguistics

Hotell Hilton Tallinn Park / Kersti Pikk

Index Scriptorium Estoniae

Pikk, Kersti

2016-01-01

Äriklassihotellide ketti Hilton kuuluv hotell Tallinnas. Arhitektuurse lahenduse autor Meelis Press. Interjööri kujundasid Soome büroo dSign Vertti Kivi & Co ja Allianss Arhitektid. Hotelli tube ja üldalasid kaunistavad Katrin Karu ja Maria Simsoni maalid

Saaremaa - kokkuhoidev kogukond

Index Scriptorium Estoniae

2004-01-01

Saaremaa Politseiprefektuuri töötajad Ants Ley, Meelis Juhandi, Hillar Peegel, Juri Krotman, Veigo Väli, Rainer Antsaar ja Riina Paju. Koostööpartneritena kommenteerivad Prits Liblik, Taivo Vaik, Toomas Luik ja Toomas Kasemaa. Vt. samas: Ajalooline Kuressaare politseimaja

Idea juht eitas maksupettust / Inge Rumessen

Index Scriptorium Estoniae

Rumessen, Inge

2002-01-01

Reklaamiagentuuri Idea juht Mark Eikner eitas kohtuistungil seost mitmemiljonilise maksupettuse skeemiga möödunud kohalike valimiste ajal. Kohtuprotsess Meelis Siidirätsepa üle, kes OÜ Rescue juhina jättis tasumata 1,5 milj. krooni käibemaksu

Suur langevari tagab pehme maandumise / Raigo Neudorf

Index Scriptorium Estoniae

Neudorf, Raigo

2003-01-01

Arutletakse, kuidas muuta riigiametitest või -ettevõtetest lahkuvatele tippjuhtidele makstavate lahkumishüvitiste süsteemi. Arvamust avaldavad rahandusministeeriumi ettevõtluse talitluse juhataja Hjalmar Liivik, AS-i Eesti Post juhatuse esimees Alo Streimann ning majandus- ja kommunikatsiooniminister Meelis Atonen.

Teadvusenähtusi tähistavad mõisted ja psühhoanalüüs Eesti moodi / Talis Bachmann

Index Scriptorium Estoniae

Bachmann, Talis, 1951-

2016-01-01

Kommentaar artiklitele: Meelis Sütt, Ants Parktal. "Mõtisklusi psühhoanalüüsi eestindamisest." - Akadeemia, 2015, nr. 7, lk. 1236-1255 ; Endel Talvik. "Psühhoanalüütiliste terminite eestindamisest: Unbewusste - alateadvus?" - Akadeemia, 2015, nr. 12, lk. 2133-2142

Dynamic evolution of the alpha (α) and beta (β) keratins has accompanied integument diversification and the adaptation of birds into novel lifestyles

DEFF Research Database (Denmark)

Greenwold, Matthew J.; Bao, Weier; Jarvis, Erich D.

2014-01-01

BACKGROUND: Vertebrate skin appendages are constructed of keratins produced by multigene families. Alpha (α) keratins are found in all vertebrates, while beta (β) keratins are found exclusively in reptiles and birds. We have studied the molecular evolution of these gene families in the genomes...... of 48 phylogenetically diverse birds and their expression in the scales and feathers of the chicken. RESULTS: We found that the total number of α-keratins is lower in birds than mammals and non-avian reptiles, yet two α-keratin genes (KRT42 and KRT75) have expanded in birds. The β-keratins, however...

Me suudame ajaga kaasas käia / interv. Ene Riet

Index Scriptorium Estoniae

2003-01-01

Eesti raamatukoguhoidjate VIII kongressi (9.-10. juuni 2002) delegaatitelt küsiti arvamust Eesti raamatukogunduse kohta, vastasid: Kalli Kuhi, Sirje Okapuu, Toomas Liivamägi, Sirje Lauring-Vaska, Helve Sreiberg, Irina Kruusla, Ädu Neemre, Tiit Margna, Valentina Brovina, Asko Tamme, Meeli Veskus

Tshto ostalos za kadrom / Jevgeni Ashihmin

Index Scriptorium Estoniae

Ashihmin, Jevgeni

2003-01-01

Sillamäe kultuurikeskuses näidati uut dokumentaalfilmi Sillamäe pensionäride ühenduse aktivisti Esja Shuri võitlusest oma õiguste eest "Meeleavaldaja" : režissöör ja produtsent Meelis Muhu : operaator Erik Norkroos : In Ruum 2003

Nõia järeltulijast Ilmari Karrost tehti tõsielufilm / Vallo Nuust

Index Scriptorium Estoniae

Nuust, Vallo, 1962-

2002-01-01

Meelis Piller on teinud tõsielufilmi Ilmari Karrost, kes räägib filmis oma linnanõiast vaaremast Katarina Blumbergist ja Äksi nõiast Emilie Elisabeth Jürgensist. Filmi näidatakse 26. aprillil Tallinnas tarbekunstimuuseumi näitusel "Nõia nõu"

Poliitilised väljapressijad / Tarmo Vahter

Index Scriptorium Estoniae

Vahter, Tarmo, 1970-

2006-01-01

Ilmunud ka: Infopress 27. okt. nr. 43 lk. 158-159. Ajakirjaniku leiud Rahvusliku Koonderakonna Isamaa 1991-1995 aastate arhiivis, erakonna sponsorid. Lisa: 451 000 krooni Isamaale. Riigiga seotud firmade annetused. Ilmunud ka: Meelis Atoneni repliiki 26. okt. Eesti Ekspress lk. A8

Lühianimafilm "Väike lühinägelik boamadu" võitis UNICEF-i auhinna

Index Scriptorium Estoniae

2007-01-01

A Film Eesti lastelühianimafilm (loo autor Andry Ervald, režissöörid Aina Järvine ja Meelis Arulepp) tunnistati parimaks haridus- ja sotsiaalteemaliseks filmiks 19.-22. aprillini Itaalias toimunud "Cartoons on the Bay" festivalil ja sai Pulcinella auhinna

Eesti põrsad Hiinas / Janno Roos

Index Scriptorium Estoniae

Roos, Janno, 1976-

2009-01-01

Shanghai EXPO Eesti paviljoni konkursi võidutööst "Savecity.org". Võidutöö autorid Illimar Truverk AET-Arhitektid, Priit Hamer AET-Arhitektid, Janno Roos Ruumilabor, Andres Labi Ruumilabor, Ionel Lehari Identity, Kristian Paljasma Identity, Meelis Opmann Identity ja Maret Põldre Identity

Valitsused on ühes voodis ärimeestega / Annika Matson

Index Scriptorium Estoniae

Matson, Annika, 1976-

2005-01-01

Ligi 30 aastat tagasi loodud kosmeetika firma The Body Shop asutajast Anita Roddick'ust. Vt. samas: Minu suurim dilemma. Graafik: The Body Shopi aktsia on Londoni börsil näinud nii häid kui ka halbu aegu. Kommenteerib Meelis Milder

Poola pole Ignalina plaane lukku löönud / Piotr Wozniak ; interv. Raimo Poom

Index Scriptorium Estoniae

Wozniak, Piotr

2007-01-01

Äsja laialisaadetud Poola valitsuse majandusministri sõnul soovib Poola olla Ignalina tuumaelektrijaama projektis aktiivne investor, st Poola soovib teatud osa selle kavandatavast võimsusest, minimaalselt 1000 MW. Meelis Atonen, Villu Reiljan ja Evelyn Sepp vastavad küsimusele, kas Ignalina suudab põlevkivienergiat asendada

Ühe sümptomi kirjeldus - Sillamäe Jeanne D'Arc / Karol Ansip

Index Scriptorium Estoniae

Ansip, Karol

2003-01-01

Dokumentaalfilm Sillamäe pensionäride ühenduse aktivisti Esja Shuri võitlusest oma õiguste eest "Meeleavaldaja" : stsenarist, režissöör ja produtsent Meelis Muhu : operaator Erik Norkroos : In Ruum 2003. Lisatud andmed M. Muhu senise loomingu kohta lk. 112

Kunst on töö hing / Signe Kivi

Index Scriptorium Estoniae

Kivi, Signe, 1957-

2009-01-01

Eesti Kunstiakadeemia 95. Kunstikõrgkooli minevikust, olevikust ja tulevikust, 95. aastapäeva tähistamisest. 10. detsembril promoveeritakse EKA audoktoriteks Helsingi ülikooli emeriitprofessor Riitta Nikula ja Leideni ülikooli graafilise disaini õppejõud Gerard Unger. Uueks EKA auliikmeks on nimetatud Meelis Milder

Kolm ministrit valgustasid Kagu-Eesti omavalitsusjuhte / Mari-Anne Leht

Index Scriptorium Estoniae

Leht, Mari-Anne, 1950-

2003-01-01

Valgamaal Taageperas räägiti Kagu-Eesti omavalitsusjuhtidele haridusest, haridusreformist ja rahast. Kohal olid ka haridus- ja teadusminister Toivo Maimets, siseminister Jaan Õunapuu ja majandusminister Meelis Atonen. Kommenteerivad Rein Randver, Pille Tali, Sirje Tobreluts jt. Vt. samas: Hannes Võrno kaebas rasket elu

Karmel Eikner seljatab Stockmanni! / Lea Veelma

Index Scriptorium Estoniae

Veelma, Lea, 1947-2007

2006-01-01

Karmel Eikneri koostöö Stockmanni kaubamajaga on lõppemas: kaubamaja korjas müügilt tema brändi Powered by Karmel tooted ja süüdistab ärinaist omavolilises allahindluses. Vandeadvokaadi Meelis Pirni sõnul on Stockmann rikkunud koostöölepingut

Eesti lastefilm sai UNICEFi auhinna

Index Scriptorium Estoniae

2007-01-01

A Film Eesti laste lühianimafilm (režissöörid Aina Järvine ja Meelis Arulepp) tunnistati parimaks haridus- ja sotsiaalteemaliseks filmiks 19.-22. aprillini Itaalias toimunud "Cartoons on the Bay" festivalil ja sai Pulcinella auhinna. Praegu luuakse selle filmi tegelastega telesarja

Näiliselt leppinud partnerid ootavad kiiresti uut ministrit / Tuuli Koch, Mirko Ojakivi

Index Scriptorium Estoniae

Koch, Tuuli

2005-01-01

Järgmiseks määravaks sammuks valitsuskoalitsiooni püsimise suhtes võib kujuneda uue välisministri kandidaadi nimetamine. Lisa: Ansip jäi oma seisukoha juurde. Vt. samas: Võitlus Meelis Atoneni ja Andrus Ansipi vahel; lühiintervjuud peaminister Juhan Partsiga

VES magas Riias

Index Scriptorium Estoniae

2002-01-01

Valie Export Society (Kadi Estland, Killu Sukmit, Marko Laimre, Meelis Salujärv) osales 9.-11. V Riias toimunud rahvusvahelisel üritusel "6th element" remake-aktsiooniga. Performance'itega esinesid Sara Lunden Rootsist, Kate Pendry Norrast, Jenia Svetlova Venemaalt, Solomia Savtshuk ja Natalia Golibroda Ukrainast

Overexpression of Akt1 enhances adipogenesis and leads to lipoma formation in zebrafish.

Directory of Open Access Journals (Sweden)

Che-Yu Chu

Full Text Available BACKGROUND: Obesity is a complex, multifactorial disorder influenced by the interaction of genetic, epigenetic, and environmental factors. Obesity increases the risk of contracting many chronic diseases or metabolic syndrome. Researchers have established several mammalian models of obesity to study its underlying mechanism. However, a lower vertebrate model for conveniently performing drug screening against obesity remains elusive. The specific aim of this study was to create a zebrafish obesity model by over expressing the insulin signaling hub of the Akt1 gene. METHODOLOGY/PRINCIPAL FINDINGS: Skin oncogenic transformation screening shows that a stable zebrafish transgenic of Tg(krt4Hsa.myrAkt1(cy18 displays severely obese phenotypes at the adult stage. In Tg(krt4:Hsa.myrAkt1(cy18, the expression of exogenous human constitutively active Akt1 (myrAkt1 can activate endogenous downstream targets of mTOR, GSK-3α/β, and 70S6K. During the embryonic to larval transitory phase, the specific over expression of myrAkt1 in skin can promote hypertrophic and hyperplastic growth. From 21 hour post-fertilization (hpf onwards, myrAkt1 transgene was ectopically expressed in several mesenchymal derived tissues. This may be the result of the integration position effect. Tg(krt4:Hsa.myrAkt1(cy18 caused a rapid increase of body weight, hyperplastic growth of adipocytes, abnormal accumulation of fat tissues, and blood glucose intolerance at the adult stage. Real-time RT-PCR analysis showed the majority of key genes on regulating adipogenesis, adipocytokine, and inflammation are highly upregulated in Tg(krt4:Hsa.myrAkt1(cy18. In contrast, the myogenesis- and skeletogenesis-related gene transcripts are significantly downregulated in Tg(krt4:Hsa.myrAkt1(cy18, suggesting that excess adipocyte differentiation occurs at the expense of other mesenchymal derived tissues. CONCLUSION/SIGNIFICANCE: Collectively, the findings of this study provide direct evidence that Akt1

Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats

Directory of Open Access Journals (Sweden)

Rasha R. Ahmed

2015-01-01

Full Text Available BACKGROUND: Impaired wound healing is a complication of diabetes and a serious problem in clinical practice. We previously found that whey protein (WP was able to regulate wound healing normally in streptozotocin (STZ-dia-betic models. This subsequent study was designed to assess the effect of WP on heat shock protein-72 (Hsp72 and keratin16 (Krt16 expression during wound healing in diabetic rats. METHODS: WP at a dosage of 100 mg/kg of body weight was orally administered daily to wounded normal and STZ-diabetic rats for 8 days. RESULTS: At day 4, the WP-treated diabetic wound was significantly reduced compared to that in the corresponding control. Diabetic wounded rats developed severe inflammatory infiltration and moderate capillary dilatation and regeneration. Treated rats had mild necrotic formation, moderate infiltration, moderate to severe capillary dilatation and regeneration, in addition to moderate epidermal formation. Hsp72 and Krt16 densities showed low and dense activity in diabetic wounded and diabetic wounded treated groups, respectively. At day 8, WP-treatment of diabetic wounded animals revealed great amelioration with complete recovery and closure of the wound. Reactivity of Hsp72 and Krt16 was reversed, showing dense and low, or medium and low, activity in the diabetic wounded and diabetic wounded treated groups, respectively. Hsp72 expression in the pancreas was found to show dense reactivity with WP-treated diabetic wound rats. CONCLUSION: This data provides evidence for the potential impact of WP in the up-regulation of Hsp72 and Krt16 in T1D, resulting in an improved wound healing process in diabetic models.

Characterization of the archaeal ribonuclease P proteins from Pyrococcus horikoshii OT3.

Science.gov (United States)

Terada, Atsushi; Honda, Takashi; Fukuhara, Hideo; Hada, Kazumasa; Kimura, Makoto

2006-08-01

Ribonuclease P (RNase P) is a ribonucleoprotein complex involved in the processing of the 5'-leader sequence of precursor tRNA (pre-tRNA). Our earlier study revealed that RNase P RNA (pRNA) and five proteins (PhoPop5, PhoRpp38, PhoRpp21, PhoRpp29, and PhoRpp30) in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 reconstituted RNase P activity that exhibits enzymatic properties like those of the authentic enzyme. In present study, we investigated involvement of the individual proteins in RNase P activity. Two particles (R-3Ps), in which pRNA was mixed with three proteins, PhoPop5, PhoRpp30, and PhoRpp38 or PhoPop5, PhoRpp30, and PhoRpp21 showed a detectable RNase P activity, and five reconstituted particles (R-4Ps) composed of pRNA and four proteins exhibited RNase P activity, albeit at reduced level compared to that of the reconstituted particle (R-5P) composed of pRNA and five proteins. Time-course analysis of the RNase P activities of R-4Ps indicated that the R-4Ps lacking PhoPop5, PhoRpp21, or PhoRpp30 had virtually reduced activity, while omission of PhoRpp29 or PhoRpp38 had a slight effect on the activity. The results indicate that the proteins contribute to RNase P activity in order of PhoPop5 > PhoRpp30 > PhoRpp21 > PhoRpp29 > PhoRpp38. It was further found that R-4Ps showed a characteristic Mg2+ ion dependency approximately identical to that of R-5P. However, R-4Ps had optimum temperature of around at 55 degrees C which is lower than 70 degrees C for R-5P. Together, it is suggested that the P. horikoshii RNase P proteins are predominantly involved in optimization of the pRNA conformation, though they are individually dispensable for RNase P activity in vitro.

Viljandi ilusamad ja inetumad uusehitised võhikute pilgu läbi / Gert Kiiler

Index Scriptorium Estoniae

Kiiler, Gert

2007-01-01

Viimase kümne aasta uusehitisi Viljandis hindavad Meelis Atonen, Jaan Tammsalu, Anu Raud, Erge Hanschmidt, Priit Aule ja sisearhitekt Hannes Praks. Ilusaimaks tunnistati Mainori keskus ja Linna Auto maja. Konkurentsis viimane oli bussijaama hoone. Lisaks Gert Kiileri "Hoone ilu sõltub paljudest nüanssidest"

ERKI moe ülevaatus pakkus täismajale toreda õhtu / Tiiu Oga

Index Scriptorium Estoniae

Oga, Tiiu

2007-01-01

Eesti Kunstiakadeemia 13.01.2007 Salme kultuurikesksuses toimunud moeetendusest. Lavastaja Kaarel Väli, graafiline kujundus Eiko Ojala, videokunstnikud Taavi Varm ja Aleks Tenusaar koos RGB-ga, lavaline kujundus Ain Nurmela, valguskunstnik Meelis Lusmägi koos ET Valgusega. Korraldajad Jaanika Terasmaa ja Ester Kannelmäe

Atonen viiks netiühenduse igasse metsatallu / Kai Kalamees

Index Scriptorium Estoniae

Kalamees, Kai

2004-01-01

Majandus- ja kommunikatsiooniminister Meelis Atonen kavatseb kohtuda Bratsislavas peetaval Kesk- ja Ida-Euroopa riikide IT-ministrite ümarlaual Microsofti juhi Bill Gatesiga, et tekitada temas huvi e-Eesti idee vastu ning teha ettepanek muuta Microsofti ärimudelit nii, et tarkvara hinnad oleksid kodutarbijatele soodsamad ja paindlikumad

Aasta betoonehitis 2005

Index Scriptorium Estoniae

2006-01-01

Aasta betoonehitiseks valiti TTP kontorihoone Tallinnas Pirital (Mähe tee 1), arhitekt Meelis Press. Eriauhinnad pälvisid arhitektuuribüroo QP Arhitektid (arhitektid Tõnu Laigu, Tõnu Laanemäe) AS Coal Terminal peahoone eest Muuga sadamas ja arhitektuuribüroo 3 + 1 Arhitektid eramu eest Magasini kvartalis Tallinnas

Ольга Темникова прглашает на одну ночь / Ксения Репсон

Index Scriptorium Estoniae

Репсон, Ксения

2009-01-01

Olga Temnikova kunstniku (naine) ja kunstiarmastaja (mees) suhet kajastavast maalinäitusest "One Night Stand" Tallinna Linnagaleriis. Kaasautorid on Kivisildnik ja Ilja Nartov. Kunstiprojekti tekstiosaks on Kivisildniku miniatuurteos „Meelis ja Vjatško”. Ilja Nartov illustreerib oma maaliloominguga olukorda, kuidas kunstiarmastaja võib hakkama saada ka ilma kunstnikuta

Saladusliku Sandu Hubani miljonid / Peeter Raidla

Index Scriptorium Estoniae

Raidla, Peeter, 1955-

2005-01-01

Eesti ühe edukaima hokiklubi Välk 494 rahastaja Sandu Hubani alias Aleksandr Gubani tegevusest, ettevõtlusest ja äritegevusest. Lisad: Hubani osalused firmades; Ettevõtted, millega Huban enam seotud ei ole; Välk 494 seob Hubani ja tema äripartnereid. Kommenteerivad Meelis Birk, Enriko Lukecha, Aivo Pärn

Investigación y práctica urbanística desde la Escuela de Arquitectura de Madrid : 20 años de actividad del Instituto Juan de Herrera (SPyOT, 1977-1997

Directory of Open Access Journals (Sweden)

Ramón López de Lucio

1997-11-01

For two decades now, research and urban practice at Escuela Técnica Superior de Arquitectura de Madrid has notably come apart from dominant planning ideology, in which "urban project" has been considered from a quite reductionist approach, so embedded in the paradigmatic tautology of "urban urbanism", as the only way of working and teaching. In 1977 a group of professors of what latter became the Departamento de Urbanística y Ordenación del Territorio de la E.T.S. de Arquitectura de Madrid decided to organize themselves as a working group with the objective of developing research projects and eventually professional work of particular interest in the fields of city and regional planning. Under the name of Seminario de Planeamiento y Ordenación del Territorio (SPyOT this group started a series of research projects focusing initially in Madrid -as central object of study and laboratory of new planning initiatives- and in the comprehensive plans of the main Spanish cities. After 20 years of uninterrupted activity the initial group has grown and consolidated, and its fields of activity have expanded. More than one hundred projects coordinated by two dozens of professors have been completed in which about 200 hundred students have cooperated. A permanent platform for research within the university has developed and an alternative for professional work for full-time professors has been created, following the guidelines of the University Reform Law (LRU. A considerable number of outstanding students have started careers in the planning field, both in the public and private sectors. After 20 years it can be said that the always difficult relationship between academia and the professional world, both for students and professors, has been facilitated by the existence of the SPyOT. Today the SPyOT has become the Sección de Urbanismo del Instituto Juan de Herrera. This publication includes, after some introductory remarks by the Directors of the Planning Department of the

Tummfilmiklassika kohtub maailmamuusikaga / Andreas Sepp

Index Scriptorium Estoniae

Sepp, Andreas

2011-01-01

Festivalist "Tummfilmid tõstavad häält", mis kestab 19.02.-5.03.2011. Filmi "Inimene filmikaameraga" (Venemaa, 1929) uushelindab Dead Combo, "Noored Kotkad" (Eesti, 1927/2008) Meelis Vind (Eesti) ja Napoleon Maddox (USA), "Berliin, suurlinna sümfoonia" (Saksamaa, 1927) ÖÄK, "Antenn" (Argentina, 2007) Argento Tango Fusion

Goskontrol : võplatõ za zagrjaznenije ne pobuzhdajut predprijatija sokratit võbrossõ / Külli Kriis

Index Scriptorium Estoniae

Kriis, Külli, 1961-

2008-01-01

Riigikontrolli hinnangul ei ole saastetasud üldjuhul välisõhu saastamist ja jäätmete tekitamist vähendanud, kuna oluliselt odavam on saastamine kinni maksta kui keskkonnasõbralikku tehnoloogiasse investeerida. Kommenteerivad ASi VKG tehnikadirektor Meelis Eldermann ja OÜ Kiviõli Keemiatööstus keskkonnakaitse peaspetsialist Kersti Salulaid

Autokool kahtlustab ARK-i kättemaksus / Antti Oolo

Index Scriptorium Estoniae

Oolo, Antti, 1967-

2000-01-01

Tallinna autokooli Radial omanik Peeter Erik näeb kooli võimaliku tegevuslitsentsist ilmajäämise taga riikliku autoregistrikeskuse kättemaksu. Veebruaris jäi Radiali töötaja Meeli Kaljusaar kaitsepolitseile vahele ebaseaduslikult väljastatud juhilubade müügiga. Tabel: Litsentsi kaotamise ohus olevad autokoolid

Isehakanud klassikute näitus / Margus Kiis

Index Scriptorium Estoniae

Kiis, Margus

2003-01-01

Fotonäitusest XXL, mis hõlmab peamiselt 1990-2000 a. fotosid, Tartu Kunstnike Majas. Näituse koostajad Peeter Linnap ja Vello Kalamets. Kirjeldatud DeStudio, Peeter Lauritsa, Toomas Kalve, Tarvo Hanno Varrese, Peeter Linnapi, Meelis Lukki, Ain Protsini, Tõnu Tamme, Malev Toomi, Vello Kalametsa ja Madis Palmi töid

Romaanid võistu rebimas - August Jakobsonist Sass Hennoni / Jaak Urmet

Index Scriptorium Estoniae

Urmet, Jaak, 1979-

2005-01-01

2004. a. romaanivõistluse esikoharomaan on Sass Henno "Mina olin siin", teine koht: Olavi Ruitlane "Kaval-Peeter ja Vene-Pagan", kolmas koht: Meelis Friedenthal "Kuldne aeg". Sisaldab võitjate nimekirja 1979-2004 ja andmeid noorte silmapaistvate autorite kohta: Kaarel Kressa, Toomas Verrev, Priit Kruus, Rolf Liiv, André Trinity, Jana Lepik,

The Estonian Tax System is Under Attack / Craig Rawlings, Daria Sivovol, Kersti Harkmann

Index Scriptorium Estoniae

Rawlings, Craig

2006-01-01

Ameerika, Briti ja Rootsi Kaubanduskojad Eestis ning Prantsuse-Eesti Äriklubi korraldasid seminari, kus Eesti maksusüsteemi tuleviku üle arutasid 15 põhiesinejat ning üle saja osavõtja. Väljavõte sessioonist, kus vastajateks olid Meelis Atonen, Eiki Nestor, Tarmo Kriis ja Vahur Kraft. Lisa: Esinejate tutvustus

The Transcriptional Landscape of p53 Signalling Pathway

Directory of Open Access Journals (Sweden)

Chizu Tanikawa

2017-06-01

Full Text Available Although recent cancer genomics studies have identified a large number of genes that were mutated in human cancers, p53 remains as the most frequently mutated gene. To further elucidate the p53-signalling network, we performed transcriptome analysis on 24 tissues in p53+/+ or p53−/− mice after whole-body X-ray irradiation. Here we found transactivation of a total of 3551 genes in one or more of the 24 tissues only in p53+/+ mice, while 2576 genes were downregulated. p53 mRNA expression level in each tissue was significantly associated with the number of genes upregulated by irradiation. Annotation using TCGA (The Cancer Genome Atlas database revealed that p53 negatively regulated mRNA expression of several cancer therapeutic targets or pathways such as BTK, SYK, and CTLA4 in breast cancer tissues. In addition, stomach exhibited the induction of Krt6, Krt16, and Krt17 as well as loricrin, an epidermal differentiation marker, after the X-ray irradiation only in p53+/+ mice, implying a mechanism to protect damaged tissues by rapid induction of differentiation. Our comprehensive transcriptome analysis elucidated tissue specific roles of p53 and its signalling networks in DNA-damage response that will enhance our understanding of cancer biology.

Suure filosoofi surm / Meelis Piller

Index Scriptorium Estoniae

Piller, Meelis

2003-01-01

Eesti päritolu mõtlejast ja joogist Rama Tammest ; Samal teemal vaata ka "Mihkel Tamm" (Haljand Udami materjalide põhjal). Ajakirjanik ja režissöör käis Mihkel (Rama) Tamme portreefilmi "Null" tarvis USAs intervjueerimas

Sündinud mittetapjaks / Meelis Kapstas

Index Scriptorium Estoniae

Kapstas, Meelis, 1963-

2002-01-01

Ilmar Raagi Hamleti-teemaline lühimängufilm "Miski on mäda ehk Kogu vale Hamletist" ("Something is Rotten or All the Lie About Hamlet") võrdluses Kenneth Branagh' suurejoonelise Hollywoodi toodanguga "Hamlet" : USA 1996. Lisa : A. Ermeli koostatud K. Branagh' filmograafia lk. 86

Muutumatud ja muutuvad / Meelis Friedenthal

Index Scriptorium Estoniae

Friedenthal, Meelis, 1973-

2016-01-01

Varauusaja nn. universaalsest inimesest. Aristotelese kümnest kategooriast. Ülikoolidest ja seal õpetatud distsipliinidest varauusajal. Distsipliinide ühisest eesmärgist - kuidas hästi ja õigesti elada. Teaduse ja religiooni vastandumine sai võimalikuks alles siis kui ühendteooria vahetus mehhanistliku maailmapildi vastu. Keskajal ja varauusajal oli võimalik vaimsetes asjades teatud kindlus, sest kõik asjad olid viidud ühistele alustele. Kaasaja ühiskonnaprobleemid ei ole lahendatavad ainult tehnokraatlike vahenditega

Kontrastide maa Brasiilia / Meelis Joost

Index Scriptorium Estoniae

Joost, Meelis

2011-01-01

Artikli autor kuulus Euroopa Majandus- ja Sotsiaalkomitee delegatsioon, mis külastas lihavõttenädalale järgneval nädalal Brasiilia Majandus– ja Sotsiaalnõukogu kutsel Brasiilia Föderatsiooni pealinna Brasíliat ning riigi esimest pealinna, praegust Bahia osariigi pealinna Salvadori, et osaleda 4. EL– Brasiilia kodanikuühiskonna ümarlaual

Teavikute ost riigihankega / Meeli Veskus

Index Scriptorium Estoniae

Veskus, Meeli, 1953-

2007-01-01

1. maist 2007 hakkas kehtima uus riigihangete seadus, mida raamatukogudes tuleb järgida teavikuid komplekteerides. Lisa: "Raamatukogu" küsib: kuidas riigihangete seadust rakendatakse? Vastavad Tallinna Keskraamatukogu direktor Kaie Holm ja Rapla Keskraamatukogu pearaamatukoguhoidja Katrin Niklus

Okupatsioon ja propaganda / Meelis Maripuu

Index Scriptorium Estoniae

Maripuu, Meelis

2008-01-01

Venemaa massiteabevahendite infost Balti riikide ja Poola okupeerimise kohta. Alates 2005. aastast on nähtav püüe dikteerida Balti riikide ajalookäsitlusi ja välja töötada moderniseeritud Venemaa poolne kontseptsioon II maailmasõja perioodi ajaloost

Suursaadik kohvrite otsas / Meelis Kapstas

Index Scriptorium Estoniae

Kapstas, Meelis, 1963-

2007-01-01

Eestist lahkuv Briti suursaadik Nigel Haywood Eestist ja eestlastest, harrastustest, kuninganna Elizabeth II Eesti visiidist, perekonnast ning asumisest diplomaatilisele tööle Iraaki. Lisad: Nigel Haywood; Tema Majesteediga lennujaamas

Loss of notochordal cell phenotype in 3D-cell cultures: implications for disc physiology and disc repair.

Science.gov (United States)

Omlor, G W; Nerlich, A G; Tirlapur, U K; Urban, J P; Guehring, T

2014-12-01

Embryonic notochordal disc nucleus cells (NC) have been identified to protect disc tissue against disc degeneration but in human beings NC phenotype gets lost with aging and the pathophysiological mechanisms are poorly understood. NC may stimulate other cells via soluble factors, and NC-conditioned medium can be used to stimulate matrix production of other disc cells and mesenchymal stem cells and thus may be of special interest for biological disc repair. As this stimulatory effect is associated with the NC phenotype, we investigated how cell morphology and gene-expression of the NC phenotype changes with time in 3D-cell culture. NC and inner annulus chondrocyte-like cells (CLC) from immature pigtails (freshly isolated cells/tissue, 3D-alginate beads, 3D-clusters) were cultured for up to 16 days under normoxia and hypoxia. Protein-expression was analysed by immunohistology and gene-expression analysis was carried out on freshly isolated cells and cultured cells. Cell morphology and proliferation were analysed by two-photon-laser-microscopy. Two-photon-laser-microscopy showed a homogenous and small CLC population in the inner annulus, which differed from the large vacuole-containing NC in the nucleus. Immunohistology found 93 % KRT8 positive cells in the nucleus and intracellular and pericellular Col2, IL6, and IL12 staining while CLC were KRT8 negative. Freshly isolated NC showed significantly higher KRT8 and CAIII but lower Col2 gene-expression than CLC. NC in 3D-cultures demonstrated significant size reduction and loss of vacuoles with culture time, all indicating a loss of the characteristic NC morphology. Hypoxia reduced the rate of decrease in NC size and vacuoles. Gene-expression of KRT8 and CAIII in NC fell significantly early in culture while Col2 did not decrease significantly within the culture period. In CLC, KRT8 and CAIII gene-expression was low and did not change noticeably in culture, whereas Col2 expression fell with time in culture. 3D

Millele pöörate eluaseme ostmisel ja üürimisel tähelepanu?

Index Scriptorium Estoniae

2005-01-01

Küsimusele vastavad Brüsselis tööd otsiv Kadri Kuusk, Sampo Panga koolitusosakonna juhataja Külli Meier, finantsinspektsiooni osakonnajuhataja Andre Nõmm, Riigikogu liige Meelis Atonen, Palivere lastekodu juhataja Külle Saar, Helsingis töötav arst Jaak Kiviloog, Narva kooliõpetaja Julia Ivanova, maksu- ja tolliameti peaspetsialist Tiiu Kilk

Raskustes ASil on OÜst vähem jaksu ellu jääda / Kadri Bank

Index Scriptorium Estoniae

Bank, Kadri

2008-01-01

Kadri Männasoo doktoritööst selgub, et kuigi aktsiaseltsidel tuleb täita suuremat omakapitalinõuet, on need osaühingutest väiksema jätkusuutlikkusega. Seaduspära kehtib, kui võrrelda varade mahult sama suuri ettevõtteid. Diagramm: AS-ide OÜ-ks ümberkujundamiste arv. Kommenteerib Meelis Mullaste

Eesti tööstuse areng on vaid kohalike ettevõtjate kätes / interv. Raul Kalev

Index Scriptorium Estoniae

2008-01-01

Ilmunud ka: Postimees : na russkom jazõke : Tehnika i Proizvodstvo 9. juuni lk. 1,4-5. Vestlusringis Eesti tööstuse perspektiividest osalesid majandus- ja kommunikatsiooniminister Juhan Parts, lehtmetalli töötleva AS Favor omanik Almar Proos, AS Baltika juht Meelis Milder ja tööandjate keskliidu juht Tarmo Kriis

Ekspert : klaas Vabadussamba valmistamiseks eriti hästi ei kõlba / Mari Kodres

Index Scriptorium Estoniae

Kodres, Mari

2008-01-01

Tallinnasse Harjumäele ja Islandi väljakule kavandatavate mälestusmärkide klaaslahendusest. Islandi väljakule on kavas avada vastupanu mälestusmärk "Murtud müür", autorid Kuulo Vahter, Ivo Lill ja Ain Rööpson. Kommenteerivad Meeli Kõiva ja klaasiinsener Aulis Bertin Soomest

Viisnurk saab üksuste müügist heal juhul 170 miljonit krooni / Annika Matson

Index Scriptorium Estoniae

Matson, Annika, 1976-

2004-01-01

AS Viisnurk müüb ehitus- ja spordikaupade divisjonid, liimpuiduvabriku ning vabrikualuse maa. Viisnurga endine tegevjuht Meelis Kukk ning konkureerivate ettevõtete esindajad prognoosivad müügist saadavat tulu. Diagramm: Suurimat tulu loodetakse kasumis ehitusmaterjalide tootmise müügist. Vt. samas: Adrenaliini armastav investor võiks Viisnurga aktsiat osta

Kaubamaja "Lemon" - Arco Ärikeskus : Estonia pst. 1, Tallinn

Index Scriptorium Estoniae

2002-01-01

1958. a. valminud hoone rekonstrueerimine. Projekteerija: EA Reng. Arhitekt Martin Aunin. Ehituskonstruktsioonid: Riho Märtson. Insenertehnilised osad: Karmo Pajo, Maarika Kurvits, Eha Treial. Valgusinstallatsioon: Meeli Kõiva. Kaupluste mööbel, kohtvalgustus: Priit Põldme, Ants Tolli, Ruth-Helene Kaasik, Hugo Mitt, Mart Vesker, Tarmo Piirmets. Projekt 2001, valmis 2002. I korruse põhiplaan, 4 vaadet

Lavakad, 23. lend / Eva Kübar

Index Scriptorium Estoniae

Kübar, Eva

2008-01-01

Eesti Muusika- ja Teatriakadeemia lavakunstikooli XXIII lennu lõpetajatest - näitlejad Marek Tammets, Ago Soots, Agnes Aaliste, Martin Mill, Andres Oja, Juss Haasma, Maarja Mitt, Meelis Põdersoo, Jekaterina Novosjolova, Elina Pähklimägi ja Kristo Viiding, dramaturgid Marion Jõepera, Mart Kase ja Maria Lee Liivak ning lavastajad Robert Annus ja Uku Uusberg

Finantskrahh kasvatas miljonilaenuvõtja kuumakset 1000 krooni võrra / Hindrek Riikoja

Index Scriptorium Estoniae

Riikoja, Hindrek

2008-01-01

Ilmunud ka: Postimees : na russkom jazõke 1. okt. 2008, lk. 6-7. Uut hoogu saanud rahvusvaheline börsikriis tõstab laenumakseid. Vt. samas: Ameerika Ühendriikide kinnisvarakriis võib võtta ka eestlaselt lõpuks töö ja kodu; Kuidas on reageerinud rahaturgude kokkukukkumisele Euroopa riigid; Kommenteerivad: Toomas Aak ja Meelis Milder

Saku Suurhall Rocca al Mares = Saku Suurhall in Rocca al Mare / Inga Raukas

Index Scriptorium Estoniae

Raukas, Inga, 1967-

2001-01-01

Peaprojekteerija, detailplaneering: Nord Projekt AS. Arhitektid Esa Viitanen (Soome), Andres Kariste, kaastöö: Meeli Truu, Loomet Raudsepp. Sisekujundus: Eero Jürgenson, Maarja Valk-Falk, Tambet Pedak, ARS Interjöörprojekt. Konstruktsioonid: Kaido Kuusiku, Tiit Arumäe, Aive Sau, Jaan Port. Projekteerimise projektijuht Andres Mikker. Projekt 1999-2001, valmis 2001. 9 ill

Innovaatiliste äriideede konkursi võitjad / Varje Sootak

Index Scriptorium Estoniae

Sootak, Varje, 1946-

2006-01-01

Ettevõtjatele, teadlastele ja üliõpilastele korraldatud NETS (New Entrepreneurs in Technology and Science) ettevõtlusprogrammi innovaatiliste äriideede konkursil selgusid 15 parimat, kellest mitmed on Tartu Ülikooli üliõpilased ja töötajad: Anton Litvinenko, Fred Valk, Madis Kauts, Mario Plaas, Marko Daljajev, Meelis Kaldalu, Reigo Ginter, Tanel Tarkmees

Suurfirmade 2008. aasta eelarved kinnitavad majanduse jahtumist / Mikk Salu

Index Scriptorium Estoniae

Salu, Mikk, 1975-

2008-01-01

Eesti suurettevõtete juhid räägivad, kuidas nad oma ettevõtte uue aasta eelarvet on planeerinud, kui palju on arvestanud palgakasvuks. Pindi Kinnisvara arvestab oma tulude 20-protsendilise langusega. Peep Sooman, Enn Saar, Vahur Roossaar, Tarmo Noop, Siim Kallast ja Meelis Milder vastavad küsimusele, millist palgatõusu nad prognoosivad 2008. aastal

Vaimse tervise simuleerijate triloogia / Pille-Riin Purje

Index Scriptorium Estoniae

Purje, Pille-Riin, 1963-

2008-01-01

2. mail Endla teatris esietendunud Martin Alguse näidendist "Janu", lavastaja, kunstnik ja muusikaline kujundaja Andres Noormets. 3. mail Rakvere Teatris esietendunud vene kirjaniku Venedikt Jerofejevi "Moskva-Petuški" ainetel tehtud lavastusest "Üks joodik sõitis rongiga", lavastaja Toomas Suuman, dramatiseerija Meelis Lainvoo. Jevgeni Griškovetsi "Linn" Theatrumis, lavastaja Lembit Peterson

Elu nagu filmis - ainult selline, mida te endale ei sooviks / Eva Kübar

Index Scriptorium Estoniae

Kübar, Eva

2009-01-01

20.-25. jaanuarini toimunud Helsingi dokumentaalfilmifestival DocPoint. Eesti filmidest oli festivalil Meelis Muhu "Aljoša". Pikemalt India filmist "India kuningas" ("King of India", režissöör Arvind Sinha), Uus-Meremaa filmist "Staarikunstnik ning sudaani kaksikud" ("The Art Star and the Sudanese twins", režissöör Pietra Brettkelly)

Sakraalne ruum / Aleksander Sprohgis

Index Scriptorium Estoniae

Sprohgis, Aleksander

2011-01-01

Töö sihiks oli kaardistada mõningaid aspekte küsimusest, kuidas inimesed kogevad jumalat. Kirjeldatakse sakraalset ruumi kui kohta, kus otsitakse tunnetuslikku kontakti, vaadeldakse inimese minapilti ja tunnetuses toimuvat, analüüsides erinevaid meeli ning lõpuks püütakse läbi isikliku kogemuse uurida ja seletada oma tunnetust sakraalses ruumis

Ilves tunnistas lõpuks Eesti majanduse kriisi / Kadrin Karner, Kärt Blumberg

Index Scriptorium Estoniae

Karner, Kadrin

2008-01-01

President Toomas Hendrik Ilvese majandusteemalisest videoläkitusest portaalis YouTube. Artiklis meenutatakse ühtlasi presidendi telepöördumist 31. detsembril 2007, kus riigipea pidas vajalikuks rahvast hoiatada kriisist rääkijate eest. Arvamust avaldavad valuutavahetusfirma Tavid nõukogu liige Meelis Atonen, ASi Eften Capital juhatuse liige Viljar Arakas, ASi Estiko nõukogu esimees Neinar Seli

Kontorimaailma uus esteetika / Ell-Maaja Randküla

Index Scriptorium Estoniae

Randküla, Ell-Maaja, 1939-2016

2006-01-01

Tallinnas Mähe tee 1 asuva TTP ärihoone sisekujundus. Autor: Meelis Press, kes on projekteerinud ka kogu puitmööbli. Ehitus: AS TTP (peatöövõtja), AS Roxor (betoonitööd. Eesti Sisearhitektide Liidu 2005. a. ühiskondliku interjööri preemia. Ill.: 3 korruste plaani, 30 värv. vaadet

Investor, mine rahaga Venemaale ja Poola / Liis Kängsepp

Index Scriptorium Estoniae

Kängsepp, Liis, 1981-

2008-01-01

Analüütikud soovitavad investeerida Venemaa, Poola, Brasiilia või USA aktsiaturgudele, Balti riikide suhtes ollakse aga umbusklikud. Vt. samas: Kas nüüd lõpuks on põhi käes? Raivo Sormunen. Aktsiaturu languse põhjused; Aktsiate ostmise A&O; Diagramm: Börside langus. Kommenteerivad Meelis Atonen, Märt Luuk, Arvo Nõges ja Jaan Talts

Tulge vaatama filme eesti kunstnikest!

Index Scriptorium Estoniae

2002-01-01

24. V Tallinna Kunstihoones filmiprogramm: Peeter Brambat "Müütide mäss", 2002 (Lembit Sarapuust); Rühm+0 (režissöör Meelis Salujärv) "Erki Kasemets", 2002; Mari Sobolev ja Jasper Zoova "The Kids of Rock'n roll", 2000; Tiina Kolgi portreesaade "Õnne sünnipäevaks, Tiina Tammetalu"

Kujundusideid / Ketlin Priilinn

Index Scriptorium Estoniae

Priilinn, Ketlin, 1982-

2006-01-01

Eesti sisearhitektide Annes Arro, Jorma Holmi, Kaire Kemp-Tishleri (kamina sepised Heigo Jelle), Andro Kööbi, Rein Lauri (seintel Anne Parmasto ja Rein Kelpmanni maalid), Taso Mähari (eramu arhitekt Emil Urbel, seinal Lemming Nageli maal "Punane joon all - Burn Out"), Eerik Olle, Meelis Pressi ja Anu Vainomäe kujundatud elutoad. 33 värv. ill

Ettevõtjatel puudub logistikast terviknägemus : parim ladu on veoauto kast / Reigo Kuivjõgi

Index Scriptorium Estoniae

Kuivjõgi, Reigo

2000-01-01

Kuna ettevõtetel puudub sageli logistika terviklik koordineerimine ja kulude analüüs, on liigsed logistikakulud firma majandustulemustele suureks koormaks. Logistikateemalisest ümarlauakohtumisest võtsid osa Tallinna Tehnikakõrgkooli prorektor Enno Lend, Tallinna Tehnikaülikooli professor Aare Uustalu, Schenker-BTL-i logistikadirektor Ain Tulvi, Schenker-BTL-i tegevdirektor Meelis Arumeel ja Eesti Kaubaliinide juhataja Tõnis Hintsov

Eurovisiooni vaheklipid tõid ETV-le Aumuna

Index Scriptorium Estoniae

2003-01-01

Mulluse Eurovisiooni lauluvõistluse Tallinna kontserdi vaheklipid said Eesti reklaamiauhindade Kuldmuna jagamisel Aumuna. Üks vaheklippidest "Saabastega kass", mille režissöörid on René Vilbre ja Meelis Arulepp, valiti maineka Annecy animafilmifestivali nominentide hulka. 2 klippi valiti Norras Fredrikstadis toimuva animafestivali programmi. Praegu teeb Rene Vilbre koos võttegrupiga Euroopa Liidu referendumi teemalisi klippe

Isolation and functional interrogation of adult human prostate epithelial stem cells at single cell resolution.

Science.gov (United States)

Hu, Wen-Yang; Hu, Dan-Ping; Xie, Lishi; Li, Ye; Majumdar, Shyama; Nonn, Larisa; Hu, Hong; Shioda, Toshi; Prins, Gail S

2017-08-01

Using primary cultures of normal human prostate epithelial cells, we developed a novel prostasphere-based, label-retention assay that permits identification and isolation of stem cells at a single cell level. Their bona fide stem cell nature was corroborated using in vitro and in vivo regenerative assays and documentation of symmetric/asymmetric division. Robust WNT10B and KRT13 levels without E-cadherin or KRT14 staining distinguished individual stem cells from daughter progenitors in spheroids. Following FACS to isolate label-retaining stem cells from label-free progenitors, RNA-seq identified unique gene signatures for the separate populations which may serve as useful biomarkers. Knockdown of KRT13 or PRAC1 reduced sphere formation and symmetric self-renewal highlighting their role in stem cell maintenance. Pathways analysis identified ribosome biogenesis and membrane estrogen-receptor signaling enriched in stem cells with NF-ĸB signaling enriched in progenitors; activities that were biologically confirmed. Further, bioassays identified heightened autophagy flux and reduced metabolism in stem cells relative to progenitors. These approaches similarly identified stem-like cells from prostate cancer specimens and prostate, breast and colon cancer cell lines suggesting wide applicability. Together, the present studies isolate and identify unique characteristics of normal human prostate stem cells and uncover processes that maintain stem cell homeostasis in the prostate gland. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Isolation and functional interrogation of adult human prostate epithelial stem cells at single cell resolution

Directory of Open Access Journals (Sweden)

Wen-Yang Hu

2017-08-01

Full Text Available Using primary cultures of normal human prostate epithelial cells, we developed a novel prostasphere-based, label-retention assay that permits identification and isolation of stem cells at a single cell level. Their bona fide stem cell nature was corroborated using in vitro and in vivo regenerative assays and documentation of symmetric/asymmetric division. Robust WNT10B and KRT13 levels without E-cadherin or KRT14 staining distinguished individual stem cells from daughter progenitors in spheroids. Following FACS to isolate label-retaining stem cells from label-free progenitors, RNA-seq identified unique gene signatures for the separate populations which may serve as useful biomarkers. Knockdown of KRT13 or PRAC1 reduced sphere formation and symmetric self-renewal highlighting their role in stem cell maintenance. Pathways analysis identified ribosome biogenesis and membrane estrogen-receptor signaling enriched in stem cells with NF-ĸB signaling enriched in progenitors; activities that were biologically confirmed. Further, bioassays identified heightened autophagy flux and reduced metabolism in stem cells relative to progenitors. These approaches similarly identified stem-like cells from prostate cancer specimens and prostate, breast and colon cancer cell lines suggesting wide applicability. Together, the present studies isolate and identify unique characteristics of normal human prostate stem cells and uncover processes that maintain stem cell homeostasis in the prostate gland.

Predicting timing of clinical outcomes in patients with chronic kidney disease and severely decreased glomerular filtration rate.

Science.gov (United States)

Grams, Morgan E; Sang, Yingying; Ballew, Shoshana H; Carrero, Juan Jesus; Djurdjev, Ognjenka; Heerspink, Hiddo J L; Ho, Kevin; Ito, Sadayoshi; Marks, Angharad; Naimark, David; Nash, Danielle M; Navaneethan, Sankar D; Sarnak, Mark; Stengel, Benedicte; Visseren, Frank L J; Wang, Angela Yee-Moon; Köttgen, Anna; Levey, Andrew S; Woodward, Mark; Eckardt, Kai-Uwe; Hemmelgarn, Brenda; Coresh, Josef

2018-03-24

Patients with chronic kidney disease and severely decreased glomerular filtration rate (GFR) are at high risk for kidney failure, cardiovascular disease (CVD) and death. Accurate estimates of risk and timing of these clinical outcomes could guide patient counseling and therapy. Therefore, we developed models using data of 264,296 individuals in 30 countries participating in the international Chronic Kidney Disease Prognosis Consortium with estimated GFR (eGFR)s under 30 ml/min/1.73m 2 . Median participant eGFR and urine albumin-to-creatinine ratio were 24 ml/min/1.73m 2 and 168 mg/g, respectively. Using competing-risk regression, random-effect meta-analysis, and Markov processes with Monte Carlo simulations, we developed two- and four-year models of the probability and timing of kidney failure requiring kidney replacement therapy (KRT), a non-fatal CVD event, and death according to age, sex, race, eGFR, albumin-to-creatinine ratio, systolic blood pressure, smoking status, diabetes mellitus, and history of CVD. Hypothetically applied to a 60-year-old white male with a history of CVD, a systolic blood pressure of 140 mmHg, an eGFR of 25 ml/min/1.73m 2 and a urine albumin-to-creatinine ratio of 1000 mg/g, the four-year model predicted a 17% chance of survival after KRT, a 17% chance of survival after a CVD event, a 4% chance of survival after both, and a 28% chance of death (9% as a first event, and 19% after another CVD event or KRT). Risk predictions for KRT showed good overall agreement with the published kidney failure risk equation, and both models were well calibrated with observed risk. Thus, commonly-measured clinical characteristics can predict the timing and occurrence of clinical outcomes in patients with severely decreased GFR. Copyright © 2018 International Society of Nephrology. Published by Elsevier Inc. All rights reserved.

Muusika : Hortus Musicus Moskvas. Eesti Rahvusmeeskoor Toulouse'is. Joaquín Rodrigo 100. Georg Ots kuuel plaadil. "Kiriku laulu- ja palveraamat" ئ 10. "Vikerraadio" heategevuskontsert. Artur Kapi ühingu esimees on Andres Uibo / Vello Mäeots

Index Scriptorium Estoniae

Mäeots, Vello, 1940-2015

2001-01-01

20. XI esines Hortus Musicus Moskvas Eesti saatkonnas. Eesti Rahvusmeeskoor kandis 15.XI Toulouse'is koos Toulouse'i Rahvusliku Linnaorkestriga ette Jean Sibeliuse "Kullervo". 22.XI tähistatakse hispaania helilooja Joaquín Rodrigo 100. sünniaastapäeva. Estonia teatri Valges saalis esitleti kogumik-CD-d "Georg Ots ئ Anthology". "Kiriku laulu- ja palveraamat" sai 30.XI 10. aastaseks. 3.XII toimub Tallinnas Kaarli kirikus "Vikerraadio" heategevuskontsert "Aita alustada elu". 26.X asutati Suure-Jaanis Rahvusvaheline Artur Kapi Ühing, mille esimeheks valiti organist Andres Uibo

Rõivatööstuse TOP 50 aastal 2005

Index Scriptorium Estoniae

2006-01-01

Vt. samas artiklid: Meelis Virkebau. Rõiva- ja tekstiilitööstuses puuduvad lihtsad lahendused; Euratex: traditsioonilise tööstuse maht üha väheneb; Ketlin Priilinn. Rõivatootja ja Baltika teeb tõhusaid edusamme ka jaekaupmehena; SG Balticum panustab vabadust hindavatele klientidele; Lenne paigutas maailma aktsiaturgudel edukalt raha. Tabel: Rõivatööstuse ettevõtete üld- ja finantsandmed

Book reviews / by Jürgen Rooste and Paul Raud

Index Scriptorium Estoniae

Rooste, Jürgen, 1979-

2016-01-01

Arvustus: Sinijärv, Karl Martin. KMSX: kuidas öelda. Tallinn Näo Kirik, 2016 ; Heinsaar, Mehis. Unistuste tappev kasvamine. Tallinn : Menu, 2016 ; Friedenthal, Meelis. Inglite keel. Tallinn : Varrak, 2016 ; Filimonov, P. I. Laulis, kuni kõik lõppes / tõlkinud Ingrid Velbaum-Staub. Tallinn : Varrak, 2016 ; Urgas, Silvia. Siht/Koht. Tartu : Elusamus, 2015 ; Kaus, Jan. Läheduste raamat. Tallinn : Verb, 2016

Ülemiste järve esitlev elegantne hotell : Lennujaama tee 2, Tallinn = An Elegant Hotel that Presents Lake Ülemiste : Lennujaama tee 2, Tallinn / Urmas Oja

Index Scriptorium Estoniae

Oja, Urmas, 1981-2012

2004-01-01

Aerofloti hotelli kohale ja osalt vana maja konstruktsioonidele ehitatud Ülemiste hotellist. Hotelli ees on parkla ja purskkaevuga väljak. Projekteerija: EA Reng, projektijuht Taavi Turvas. Arhitekt Martin Aunin. Sisekujundus: Meelis Press, Hillar Mänd (M. Pressi Arhitektuuribüroo). Konstruktsioonid: Inseneribüroo Pike. Ehitaja: FKSM. I, II ja III korruse plaan, 3 välis- ja 5 sisevaadet

Nad pakuvad eri lahendusi / Monica Raud

Index Scriptorium Estoniae

Raud, Monica, 1981-

2004-01-01

Ajakirjade Kirjastuse müügijuht Kristiina Anvelt, TV3 müügidirektor Meelis Järvela, Eesti Päevalehe müügijuht Krista Liiv ja vanem projektijuht Olari Raig, Delfi müügi- ja marketingijuht Allan Sombri ja Eesti Raadio müügijuht Ave Vahemets selgitavad, miks reklaamida jogurtit, spordiklubi ning pesupublit just nende kanali vahendusel

Konkurss vindus osalejate puuduses / Kristiina Randmaa

Index Scriptorium Estoniae

Randmaa, Kristiina

2003-01-01

Kvaliteediauhinna konkurss läks riigile maksma 700 000 krooni, kuigi konkursil osales vaid 6 ettevõtet. Seetõttu võidakse tulevikus kaaluda ürituse tasuliseks muutmist ettevõtete jaoks. Foto: Kvaliteediauhinna lõputseremoonial 16. oktoobril olid kohal ka majandusminister Meelis Atonen ja president Arnold Rüütel, nende kõrval auhinna võitja TNT Eesti juht Asko TaluDiagramm: Enim kulus eksperttasudele

Genetics Home Reference: Dowling-Degos disease

Science.gov (United States)

... can be triggered by UV light, sweating, or friction on the skin. The pigmentation changes characteristic of ... A, Ruzicka T, Betz RC, Hanneken S. The First Report of KRT5 Mutation Underlying Acantholytic Dowling-Degos Disease ...

Genetic and bibliographic information: KRT5 [GenLibi

Lifescience Database Archive (English)

Full Text Available nd Neonatal Diseases and Abnormalities (C16) > Congenital Abnormalities (C16.131) > Skin Abnormalities (C16....ases, Inborn (C16.320) > Skin Diseases, Genetic (C16.320.850) > Epidermolysis Bullosa (C16.320.850.275) > Ep...idermolysis Bullosa Simplex (C16.320.850.275.180) Skin and Connective Tissue Diseases (C17) > Skin Diseases (C17.800) > Skin....493) > Epidermolysis Bullosa Simplex (C17.800.804.493.180) Skin and Connective Tissue Diseases (C17) > Skin Diseases (C17.800) > Ski...rmolysis Bullosa Simplex (C17.800.827.275.180) Skin and Connective Tissue Diseases (C17) > Skin

Patrones de relación entre información biológica e información física y socioeconómica : cuenca alta del Río Otún, Risaralda Colombia

OpenAIRE

Coronel Picón, Yulieth Rossio

2009-01-01

En este trabajo se realizó un análisis de información física, socioeconómica y biológica de la cuenca del río Otún. Se presenta la metodología utilizada para la identificación de patrones de relación entre la información física y socioeconómica de la cuenca y la presencia de algunos mamíferos identificados en esta zona, y los resultados obtenidos. La identificación de patrones se llevó a cabo mediante la aplicación de árboles de decisión, utilizando el algoritmo j-48 desarrollado en el entorn...

Erythrodermia Congenitalis Ichthyosiformis Bullosa of Brocq

Directory of Open Access Journals (Sweden)

Dorothea Sander

2016-01-01

Full Text Available A 50-year-old man presented with congenital scaling and hyperkeratosis on his palms, the soles of his feet and the extensor areas of his joints. The flexural areas were unaffected. His maternal grandmother, questionably his maternal uncle, his mother, all three brothers, one of his two sisters as well as two nephews and three nieces have or had similar skin changes. A punch biopsy was taken from the left palm. Clinical and histological signs led to the diagnosis of erythrodermia congenitalis ichthyosiformis bullosa of Brocq. We confirmed this genetically and found a heterozygous duplication (c.1752dupT in the keratin 1 gene (KRT-1. To our knowledge, this is the first case of this skin condition reported in the literature with a heterozygous duplication (c.1752dupT in KRT-1.

Muretsemise mõjust kirjandusretseptsioonile / Meelis Oidsalu

Index Scriptorium Estoniae

Oidsalu, Meelis, 1978-

2010-01-01

2000-ndate eesti kirjanduse diskussioonist (ilmus peamiselt ajakirjas Looming). Ka Mihkel Kunnuse arvustusest "Tragöödia sünd heaolu talumatust ebaheroilisusest", Sirp, 17. sept., mis käsitles Tuuli Tauli luulekogu "Täiuslik ja turvaline"

Selgusid aasta 2010 tegijad / Meelis Kaubi

Index Scriptorium Estoniae

Kaubi, Meelis

2010-01-01

Saare maakonna aasta õppijaks valiti Rehe Pagar OÜ juhataja Glaudja Majorova, Aasta koolitajaks Signe Sarah Arro, Aasta koolitussõbralikumaks organisatsiooniks Trelleborg IP Estonia ja Aasta koolitussõbralikumaks omavalitsuseks Pöide vald

Külalised hommikumaalt / Meeli Parijõgi

Index Scriptorium Estoniae

Parijõgi, Meeli

2013-01-01

7.-13. maini olid Eestis õppereisil kaks Afganistani naisõpetajat - Fatema Mohammad Aziz Shaheed Mazari 1. keskkoolist ning Farida Mohammad Yunus Noor Khuda koolist. Reis oli osa arenguabiprojektist, mis parandab hariduse kättesaadavust ja kvaliteeti Afganistani maapiirkondades

Arutlus autorist ja ruumist / Meelis Oidsalu

Index Scriptorium Estoniae

Oidsalu, Meelis, 1978-

2010-01-01

Dokumentaalfilmi sarjas "Eesti lood" linastunud filmidest: "Beebilõust" - Saaremaa poeedist ja hiphop-muusikust Andrus Elbingust, režissöör ja stsenarist Margit Lillak ning "Pastacas" - Soomes elavast Eesti kunstnikust ja muusikust Ramo Tedrest, režissöör Rein Kotov

Results of investigations conducted in the LaRC 4-foot unitary plan wind tunnel leg no. 1 using the 0.010-scale 72-OTS model of the space shuttle integrated vehicle (IA94A)

Science.gov (United States)

Nichols, M. E.

1976-01-01

Aero-loads investigations were conducted on the updated configuration-5 space shuttle launch vehicle at Mach numbers 2.50, 3.50, and 4.50. Six-component vehicle forces and moments, base and sting-cavity pressures, elevon hinge moments, wing-root bending and torsion moments, and normal shear force data were obtained. Full simulation of updated vehicle protuberances and attach hardware was employed. Various elevon deflection angles were tested, with two different forward orbiter-to-external-tank attach-strut configurations. The entire vehicle model 72-OTS was supported by means of a balance mounted in the orbiter through its base and suspended from an appropriate sting for the specific tunnel.

Urinary and plasma oxytocin changes in response to MDMA or intranasal oxytocin administration.

Science.gov (United States)

Francis, Sunday M; Kirkpatrick, Matthew G; de Wit, Harriet; Jacob, Suma

2016-12-01

The neuropeptide oxytocin (OT) has received increased experimental attention for its putative role in both normal social functioning and several psychiatric disorders that are partially characterized by social dysfunction (e.g., autism spectrum disorders: ASD). Many human experimental studies measure circulating plasma levels of OT in order to examine the relationship between the hormone and behavior. Urinary OT (uOT) assays offer a simple, easy, and non-invasive method to measure peripheral hormone levels, but the correspondence between uOT and plasma OT (pOT) levels is unclear. Here, we conducted two within-subjects, double-blind studies exploring changes in uOT and pOT levels following administration of two drugs: MDMA, an oxytocin-releasing drug (Study 1), and intranasal oxytocin (INOT: Study 1 and 2). In Study 1, 14 adult participants (2 females) were each administered either oral 1.5mg/kg MDMA or 40IU INOT across two different study sessions. In Study 2, 10 male participants (adolescents and young adults) diagnosed with ASD received either 40IU INOT or placebo across two different sessions. In both studies, blood and urine samples were collected before and after drug administration at each study session. For Study 1, 10 participants provided valid plasma and urine samples for the MDMA session, and 8 provided valid samples for the INOT session. For Study 2, all 10 participants provided valid samples for both INOT and placebo sessions. Pre- and post-administration levels of pOT and uOT were compared. Additionally, correlations between percent change from baseline uOT and pOT levels were examined. Study 1: Plasma OT and uOT levels significantly increased after administration of MDMA and INOT. Furthermore, uOT levels were positively correlated with pOT levels following administration of MDMA (r=0.57, p=0.042) but not INOT (r=0.51, p=0.097). Study 2: There was a significant increase in uOT levels after administration of INOT, but not after administration of

Lühinägelik boamaopoiss müttab festivalidel

Index Scriptorium Estoniae

2007-01-01

A Film Eesti joonisfilm "Väike lühinägelik boamadu" (loo autor Andry Ervald, režissöörid Aina Järvine ja Meelis Arulepp) on üks viiest Pulcinella auhinna 2007 nominendist Itaalia animafilmifestivalil Cartoons on the Bay. Detsembris 2006 osales film IX olümpia rahvusvahelisel laste- ja noortefilmide festivalil, kus žürii valis ta parimaks lühianimafilmiks

Kõik ei ole action / Mari Sobolev

Index Scriptorium Estoniae

Sobolev, Mari, 1968-

1998-01-01

Noorte videoprojektidest. Marko Laimre ja Andrus Kõresaare "Egoistically Correct", ainus actionil rajanev video. Jasper Zoova "Sheeps Are Everywhere". J. Zoova ja Tarrvi Laamannni "Fuck". Karol Kallase kureeritud näitusel "Kunstnik tapab koletise" "Kullo" galeriis olnud Jan Bergi, Meelis Salujärve, Al Paldroki videod, Andrus Joonase töö põhjal tehtud Kiwa video "Kunstnik tapab koletise". Publiku soov vaatemängu järele.

Seitse luuleraamatut, seitse debüüti / Veiko Märka

Index Scriptorium Estoniae

Märka, Veiko, 1964-

2006-01-01

Arvustus: Teede, Andra. Takso Tallinna taevas. Luige : Verb, 2006 ; Vabat, Martin. Mina olengi kirjandusklassik. Tartu : Eesti Kirjanduse Selts, 2006 ; Kangro, Maarja. Kurat õrnal lumel. Luige : Verb, 2006 ; Ojasaar, Krista. Hommikused unenäod. Tartu : Ilmamaa, 2006 ; Korts, Eliina. Lööklaused murravad metsi. Tartu : Eesti Kirjanduse Selts, 2006 ; Piik, Paavo. Kummuli linnad. Tallinn : Tuum, 2006 ; Oidsalu, Meelis. Jumala kihvt luulekogu. Tallinn : Sirp ja Vaseliin, 2006

Testimonios arqueológicos del Oriente Próximo reflejados en el Séfer–Masa`ot de Benjamín de Tudela (Siria-Palestina, Mesopotamia y Egipto

Directory of Open Access Journals (Sweden)

Magdalena Nom de Déu, José Ramón

2005-04-01

Full Text Available Benjamín de Tudela (en hebreo Biniamín ben rabbí Yonah mi- Tudela es, sin duda, un navarro universal. Judío de la aljama tudelana, se ignora la fecha de su nacimiento (acaso hacia 1130. Hijo de rabino y viajero infatigable, sólo sabemos de este personaje lo poco que de él se menciona en su famoso Libro de Viajes o Séfer-masa`ot, fruto de sus vivas experiencias —las más de las veces directas— exquisitamente plasmadas en su relación de viaje, redactadas en hebreo. Conocedor del romance navarro vernáculo de la época, sabía hebreo - su lengua de instrucción y cultivo literario- y árabe, entendiendo acaso el griego y el latín.…

Mutations in POGLUT1, Encoding Protein O-Glucosyltransferase 1, Cause Autosomal-Dominant Dowling-Degos Disease

DEFF Research Database (Denmark)

Basmanav, F Buket; Oprisoreanu, Ana-Maria; Pasternack, Sandra M

2014-01-01

Dowling-Degos disease (DDD) is an autosomal-dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation. We previously identified loss-of-function mutations in KRT5 but were only able to detect pathogenic mutations in fewer than half of our subjects. To ident...

"Asumi" nurgalahendus tänapäeval = Corner solution of "Asum" today / Roman Koolmar

Index Scriptorium Estoniae

Koolmar, Roman, 1904-1971

2015-01-01

1937. a. projekteeritud Teedeministeeriumi ametnike korteriühing "Ühiskodu" ja kohtunike korteriühing "Asum" Raua põik 3 ja 5. Välisvaade tänavalt ja sisehoovist. Korruse plaanid. Lk. 65 "Ühiskodu" I korrusel, krt. 2 elanud Koolmarite korteri kaminanurk

Molecular constituents of the extracellular matrix in rat liver mounting a hepatic progenitor cell response for tissue repair

DEFF Research Database (Denmark)

Vestentoft, Peter Siig; Jelnes, Peter; Andersen, Jesper Bøje

2013-01-01

differentially up- or down-modulated (P tier of defense (Agrn, Bgn, Fbn1, Col4a1, Col8a1, Col9a3, Lama5, Lamb1, Lamb2, Itga4, Igtb2, Itgb4, Itgb6, Nid2), and their signal intensities showed a strong or very...... strong correlation with Krt1- 19, a well-established marker of a ductular/HPC reaction. Furthermore, a significant up-modulation and very strong correlation between the transcriptional profiles of Krt1-19 and St14 encoding matriptase, a component of a novel protease system, was found in the second tier...... that laminin, Collagen1a1, agrin and nidogen1 surrounded bile ducts, proliferating cholangiocytes, and HPCs in ductular reactions regardless of the nature of defense. Similarly, matriptase and HAI-1 were expressed in cholangiocytes regardless of the tier of defense, but in the second tier of defense...

Results of a M = 5.3 heat transfer test of the integrated vehicle using phase-change paint techniques on the 0.0175-scale model 56-OTS in the NASA/Ames Research Center 3.5-foot hypersonic wind tunnel

Science.gov (United States)

Marroquin, J.

1985-01-01

An experimental investigation was performed in the NASA/Ames Research Center 3.5-foot Hypersonic Wind Tunnel to obtain supersonic heat-distribution data in areas between the orbiter and external tank using phase-change paint techniques. The tests used Novamide SSV Model 56-OTS in the first and second-stage ascent configurations. Data were obtained at a nominal Mach number of 5.3 and a Reynolds number per foot of 5 x 10 to the 6th power with angles of attack of 0 deg, +/- 5 deg, and sideslip angles of 0 deg and +/- 5 deg.

Human forniceal region is the stem cell-rich zone of the conjunctival epithelium.

Science.gov (United States)

Harun, Mohd Hairul Nizam; Sepian, Siti Norzalehawati; Chua, Kien-Hui; Ropilah, Abd Rahman; Abd Ghafar, Norzana; Che-Hamzah, Jemaima; Bt Hj Idrus, Ruszymah; Annuar, Faridah Hanom

2013-03-01

The anterior surface of the eye is covered by several physically contiguous but histologically distinguishable epithelia overlying the cornea, limbus, bulbar conjunctiva, fornix conjunctiva, and palpebral conjunctiva. The self-renewing nature of the conjunctival epithelia makes their long-term survival ultimately dependent on small populations of stem cells. Hence, the objective of this study was to investigate the expression of the stem cell genes Sox2, OCT4, NANOG, Rex1, NES, and ABCG2 in cultured human conjunctival epithelium from different conjunctival zones, namely, the bulbar, palpebral and fornix zones. Three samples were taken from patients with primary pterygium and cataract (age range 56-66 years) who presented to our eye clinic at the UKM Medical Centre. The eye was examined with slit lamp to ensure there was no underlying ocular surface diseases and glaucoma. Conjunctival tissue was taken from patients who underwent a standard cataract or pterygium operation as a primary procedure. Tissues were digested, cultured, and propagated until an adequate number of cells was obtained. Total RNA was extracted and subjected to expression analysis of conjunctival epithelium genes (KRT4, KRT13, KRT19) and stem cell genes (Sox2, OCT4, NANOG, Rex1, NES, ABCG2) by reverse transcriptase-PCR and 2% agarose gel electrophoresis. The expression of Sox2, OCT4, and NANOG genes were detected in the fornical cells, while bulbar cells only expressed Sox2 and palpebral cells only expressed OCT4. Based on these results, the human forniceal region expresses a higher number of stem cell genes than the palpebral and bulbar conjunctiva.

Uudised : Tüüri esiettekanne Frankfurdis. Corelli Musicult "Jõulud mõisas"

Index Scriptorium Estoniae

2006-01-01

22., 23. ja 24. nov. Frankfurdi Alte Operis esiettekandele tulevast Erkki-Sven Tüüri uuest "Klaverikontserdist". Eesti-Tšiili trio Raivo Tafenau - Meelis Vind - Ricardo Padilla kontsertidest sarja "Mõisaromantika" raames 25. nov. Taagepera lossis Valgamaal, 26. nov. Tõstamaa mõisas Pärnumaal ja 16. dets. Vihterpalu mõisas Harjumaal. Hans Neuenfelsi lavastatud Mozarti ooperist "Idemeneo" Deutsche Operis Berliinis. Inglise ansambli Procol Harumi hiti "A Whiter Shade of Pale" autoriõigustest

A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing.

Science.gov (United States)

Eliava, Marina; Melchior, Meggane; Knobloch-Bollmann, H Sophie; Wahis, Jérôme; da Silva Gouveia, Miriam; Tang, Yan; Ciobanu, Alexandru Cristian; Triana Del Rio, Rodrigo; Roth, Lena C; Althammer, Ferdinand; Chavant, Virginie; Goumon, Yannick; Gruber, Tim; Petit-Demoulière, Nathalie; Busnelli, Marta; Chini, Bice; Tan, Linette L; Mitre, Mariela; Froemke, Robert C; Chao, Moses V; Giese, Günter; Sprengel, Rolf; Kuner, Rohini; Poisbeau, Pierrick; Seeburg, Peter H; Stoop, Ron; Charlet, Alexandre; Grinevich, Valery

2016-03-16

Oxytocin (OT) is a neuropeptide elaborated by the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei. Magnocellular OT neurons of these nuclei innervate numerous forebrain regions and release OT into the blood from the posterior pituitary. The PVN also harbors parvocellular OT cells that project to the brainstem and spinal cord, but their function has not been directly assessed. Here, we identified a subset of approximately 30 parvocellular OT neurons, with collateral projections onto magnocellular OT neurons and neurons of deep layers of the spinal cord. Evoked OT release from these OT neurons suppresses nociception and promotes analgesia in an animal model of inflammatory pain. Our findings identify a new population of OT neurons that modulates nociception in a two tier process: (1) directly by release of OT from axons onto sensory spinal cord neurons and inhibiting their activity and (2) indirectly by stimulating OT release from SON neurons into the periphery. Copyright © 2016 Elsevier Inc. All rights reserved.

Effect of oxytocin injection to milching buffaloes on its content & stability in milk

Directory of Open Access Journals (Sweden)

Raghu Pullakhandam

2014-01-01

Full Text Available Background & objectives: Oxytocin (OT injections to milch cattle for milk letdown have become a common practice amongst dairy farmers in India. Although there is no reported evidence, it is widely presumed that long term consumption of such milk leads to adverse health consequences. However, there is no information on the effect of exogenous OT injections on milk OT content and its stability during heating and gastrointestinal digestion. This study was carried out to determine the OT content in milk samples given by buffaloes with and without OT injections and to assess the stability of OT in the milk. Methods: Milk samples from milch buffaloes (Murrah buffalo were collected from local farmers with (n=121 or without (n=120 exogenous OT injections during 3 to 5 months of lactation period. The OT content of milk samples was estimated by competitive enzyme immunoassay (EIA. The thermal and digestive stability of OT was assessed by in silico and in vitro digestion methods. Results: The OT content of the milk samples was similar regardless of OT injections used. Further, OT was found to be stable to heat treatment and gastric pepsin digestion while it was rapidly digested during the simulated intestinal digestion. r0 educed OT was digested by pepsin, implying that internal disulphide bridge of OT rendered the peptide resistant to peptic digestion. On the other hand, phenylmethylsulphonyl fluoride (PMSF, a serine protease inhibitor, abrogated the pancreatin induced digestion of OT. Interpretation & conclusions: These findings suggest that exogenous OT injections do not influence its content in milk. Further, OT present in milk is rapidly degraded during intestinal digestion, ruling out its intestinal absorption and associated adverse health consequences, if any.

Effect of different doses of oxytocin on cardiac electrophysiology and arrhythmias induced by ischemia.

Science.gov (United States)

Houshmand, Fariba; Faghihi, Mahdieh; Imani, Alireza; Kheiri, Soleiman

2017-01-01

The onset of acute myocardial ischemia (MI) is accompanied by a rapid increase in electrical instability and often fatal ventricular arrhythmias. This study investigated that whether oxytocin (OT) can modulate ischemia-induced arrhythmias and considered relationships between the severity of arrhythmia and the electrocardiogram parameters during ischemia. OT (0.0001-1 μg) was administrated intraperitoneally 30 min before ischemia. To examine receptor involved, a selective OT-receptor antagonist, atosiban (ATO), was infused 10 min before OT. OT caused a significant and biphasic dose-dependent reduction in ectopic heart activity and arrhythmia score. OT doses that reduced ventricular arrhythmia elicited significant increase in QT interval. OT attenuated the electrophysiological changes associated with MI and there was significant direct relationship between QRS duration and arrhythmia score. ATO treatment reduced beneficial effects of OT on arrhythmogenesis. Nevertheless, ATO failed to alter OT effects on premature ventricular contractions. We assume that the ability of OT to modulate the electrical activity of the heart may play an important role in the antiarrhythmic actions of OT.

Effect of different doses of oxytocin on cardiac electrophysiology and arrhythmias induced by ischemia

Directory of Open Access Journals (Sweden)

Fariba Houshmand

2017-01-01

Full Text Available The onset of acute myocardial ischemia (MI is accompanied by a rapid increase in electrical instability and often fatal ventricular arrhythmias. This study investigated that whether oxytocin (OT can modulate ischemia-induced arrhythmias and considered relationships between the severity of arrhythmia and the electrocardiogram parameters during ischemia. OT (0.0001–1 μg was administrated intraperitoneally 30 min before ischemia. To examine receptor involved, a selective OT-receptor antagonist, atosiban (ATO, was infused 10 min before OT. OT caused a significant and biphasic dose-dependent reduction in ectopic heart activity and arrhythmia score. OT doses that reduced ventricular arrhythmia elicited significant increase in QT interval. OT attenuated the electrophysiological changes associated with MI and there was significant direct relationship between QRS duration and arrhythmia score. ATO treatment reduced beneficial effects of OT on arrhythmogenesis. Nevertheless, ATO failed to alter OT effects on premature ventricular contractions. We assume that the ability of OT to modulate the electrical activity of the heart may play an important role in the antiarrhythmic actions of OT.

Major Meelis Kiili jääb koduõuele toodud sõjaga rahule / Meelis Kiili ; interv. Gerli Romanovitsh

Index Scriptorium Estoniae

Kiili, Meelis, 1965-

2004-01-01

Ilmunud ka: Severnoje Poberezhje : Subbota, 22. mai 2004, lk. 5. Suurõppuse "Kevadtorm 2004" staabiülem annab hinnangud õppusele, kommenteerib Eesti liitumist NATO-ga ja sõjafilmide vastavust tegelikkusele

ESL aastapreemia 2005 žürii. ESL aastapreemia 2005 žürii koosoleku protokoll : 8. märts 2006 / Mari Kurismaa

Index Scriptorium Estoniae

Kurismaa, Mari, 1956-

2006-01-01

2005. a. publitsistikapreemia Leila Pärtelpojale raamatu "Pööningul. Auf dem Dachboden" eest, näitusekujunduse preemia ühendusele Laika, Belka &Strelka Eesti Maanteemuuseumi näituse "Tee ajalugu" eest, ajaloolise interjööri preemia Juta Lemberile Rahvusooper Estonia publikuruumide kujunduse eest, ühiskondliku interjööri preemia Katrin Kaevatsile ja Peeter Perele Pääsküla raamatukogu interjööri eest ning Meelis Pressile TTP ärihoone eest

Ott Puuraid 100 / Mart Kalm

Index Scriptorium Estoniae

Kalm, Mart, 1961-

2004-01-01

23. IV 2004. a. tähistati Viljandis kauaaegse linna peaarhitekti Ott Puuraiu (1904-1986) 100. sünniaastapäeva. Esinesid Mart Kalm, Ülo Stöör ja arhitekti tütar Meeli Roosalu. Kondase keskuses avati O. Puuraiu projektide näitus, mille koostas tema lapselaps, arhitekt Priit Roosalu, kes oli ka kataloogi koostanud. Näituse koostamisel olid abiks Anne Lass ja Tarmo Maiste. O Puuraiu elust ja loomingust

Identification of a novel amino acid racemase from a hyperthermophilic archaeon Pyrococcus horikoshii OT-3 induced by D-amino acids.

Science.gov (United States)

Kawakami, Ryushi; Ohmori, Taketo; Sakuraba, Haruhiko; Ohshima, Toshihisa

2015-08-01

To date, there have been few reports analyzing the amino acid requirement for growth of hyperthermophilic archaea. We here found that the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 requires Thr, Leu, Val, Phe, Tyr, Trp, His and Arg in the medium for growth, and shows slow growth in medium lacking Met or Ile. This largely corresponds to the presence, or absence, of genes related to amino acid biosynthesis in its genome, though there are exceptions. The amino acid requirements were dramatically lost by addition of D-isomers of Met, Leu, Val, allo-Ile, Phe, Tyr, Trp and Arg. Tracer analysis using (14)C-labeled D-Trp showed that D-Trp in the medium was used as a protein component in the cells, suggesting the presence of D-amino acid metabolic enzymes. Pyridoxal 5'-phosphate (PLP)-dependent racemase activity toward Met, Leu and Phe was detected in crude extract of P. horikoshii and was enhanced in cells grown in the medium supplemented with D-amino acids, especially D-allo-Ile. The gene encoding the racemase was narrowed down to one open reading frame on the basis of enzyme purification from P. horikoshii cells, and the recombinant enzyme exhibited PLP-dependent racemase activity toward several amino acids, including Met, Leu and Phe, but not Pro, Asp or Glu. This is the first report showing the presence in a hyperthermophilic archaeon of a PLP-dependent amino acid racemase with broad substrate specificity that is likely responsible for utilization of D-amino acids for growth.

Bērnu mārketinga attīstība Latvijas lielāko pārtikas produktu ražotāju tirgus darbībās

OpenAIRE

Lūse, Ieva

2010-01-01

Uzņēmumi visā pasaulē arvien vairāk sāka apzināties bērnu potenciālu tirgū un aktivizē savu komunikāciju ar bērnu auditoriju. Bērni vienlaicīgi tirgū pilda vairākas lomas kā patērētāji, iniciatori, ietekmētāji un lēmēji par pirkumu un pircēji šodien un nākotnē, kļūstot par daudzu uzņēmumu produkcijas pamata patērētājiem. Ņemot vērā tēmas aktualitāti autore par maģistra darba mērķi izvirza apzināt un analizēt lielāko Latvijas pārtikas produktu ražotāju aktivitāti bērnu mārketing...

Oxytocin and vasopressin flatten dominance hierarchy and enhance behavioral synchrony in part via anterior cingulate cortex.

Science.gov (United States)

Jiang, Yaoguang; Platt, Michael L

2018-05-29

The neuropeptides oxytocin (OT) and arginine vasopressin (AVP) influence social functions in many mammals. In humans and rhesus macaques, OT delivered intranasally can promote prosocial behavior in certain contexts. Yet the precise neural mechanisms mediating these behavioral effects remain unclear. Here we show that treating a group of male macaque monkeys intranasally with aerosolized OT relaxes their spontaneous social interactions with other monkeys. OT reduces differences in social behavior between dominant and subordinate monkeys, thereby flattening the status hierarchy. OT also increases behavioral synchrony within a pair. Intranasal delivery of aerosolized AVP reproduces the effects of OT with greater efficacy. Remarkably, all behavioral effects are replicated when OT or AVP is injected focally into the anterior cingulate gyrus (ACCg), a brain area linked to empathy and other-regarding behavior. ACCg lacks OT receptors but is rich in AVP receptors, suggesting exogenous OT may shape social behavior, in part, via nonspecific binding. Notably, OT and AVP alter behaviors of both the treated monkey and his untreated partner, consistent with enhanced feedback through reciprocal social interactions. These findings bear important implications for use of OT in both basic research and as a therapy for social impairments in neurodevelopmental disorders.

Modifications to the restriction method of Gentry and Casanas for otariid pups Modificaciones al método de restricción de Gentry y Casanas para crías de otáridos

Directory of Open Access Journals (Sweden)

Janitzio Égido-Villarreal

2007-06-01

Full Text Available To avoid risky and expensive chemical immobilization of otariid pups, physical restriction is a better option. For this reason we constructed a stretcher that permits wide liberty to interact with the pup and take a variety of samples in a safe, comfortable and easy way, for both the pup and the researcher.Para evitar el uso de la riesgosa y cara restricción química en crías de otáridos la mejor opción es la restricción física. Por lo anterior construimos una camilla que permite amplia libertad para la toma de diferentes tipos de muestras de manera segura, cómoda y fácil tanto para la cría como para el investigador.

Body composition, metabolism, sleep, psychological and eating patterns of overtraining syndrome: Results of the EROS study (EROS-PROFILE).

Science.gov (United States)

Cadegiani, Flavio A; Kater, Claudio E

2018-08-01

Overtraining syndrome (OTS) is caused by an imbalance between training, nutrition and resting, and leads to decreased performance and fatigue; however, the precise underlying triggers of OTS remain unclear. This study investigated the body composition, metabolism, eating, sleeping patterns and mood states among participants with OTS. Selected participants were divided into OTS-affected athletes (OTS, n = 14), healthy athletes (ATL, n = 25), and healthy non-physically active controls (NCS, n = 12). Compared to ATL, OTS showed decreased sleep quality (p = 0.004); increased duration of work or study (p sleep, and increased cognitive activity are likely OTS triggers. OTS appears to induce dehydration, increase body fat, decrease libido, and worsen mood.

Oxytocin in the prelimbic medial prefrontal cortex reduces anxiety-like behavior in female and male rats.

Science.gov (United States)

Sabihi, Sara; Durosko, Nicole E; Dong, Shirley M; Leuner, Benedetta

2014-07-01

The neuropeptide oxytocin (OT) is anxiolytic in rodents and humans. However, the specific brain regions where OT acts to regulate anxiety requires further investigation. The medial prefrontal cortex (mPFC) has been shown to play a role in the modulation of anxiety-related behavior. In addition, the mPFC contains OT-sensitive neurons, expresses OT receptors, and receives long range axonal projections from OT-producing neurons in the hypothalamus, suggesting that the mPFC may be a target where OT acts to diminish anxiety. To investigate this possibility, female rats were administered OT bilaterally into the prelimbic (PL) region of the mPFC and anxiety-like behavior assessed. In addition, to determine if the effects of OT on anxiety-like behavior are sex dependent and to evaluate the specificity of OT, male and female anxiety-like behavior was tested following delivery of either OT or the closely related neuropeptide arginine vasopressin (AVP) into the PL mPFC. Finally, the importance of endogenous OT in the regulation of anxiety-like behavior was examined in male and female rats that received PL infusions of an OT receptor antagonist (OTR-A). Overall, even though males and females showed some differences in their baseline levels of anxiety-like behavior, OT in the PL region of the mPFC decreased anxiety regardless of sex. In contrast, neither AVP nor an OTR-A affected anxiety-like behavior in males or females. Together, these findings suggest that although endogenous OT in the PL region of the mPFC does not influence anxiety, the PL mPFC is a site where exogenous OT may act to attenuate anxiety-related behavior independent of sex. Copyright © 2014 Elsevier Ltd. All rights reserved.

Sex-Specific Effects of Stress on Oxytocin Neurons Correspond With Responses to Intranasal Oxytocin.

Science.gov (United States)

Steinman, Michael Q; Duque-Wilckens, Natalia; Greenberg, Gian D; Hao, Rebecca; Campi, Katharine L; Laredo, Sarah A; Laman-Maharg, Abigail; Manning, Claire E; Doig, Ian E; Lopez, Eduardo M; Walch, Keenan; Bales, Karen L; Trainor, Brian C

2016-09-01

Oxytocin (OT) is considered to be a stress-buffering hormone, dampening the physiologic effects of stress. However, OT can also be anxiogenic. We examined acute and long-lasting effects of social defeat on OT neurons in male and female California mice. We used immunohistochemistry for OT and c-fos cells to examine OT neuron activity immediately after defeat (n = 6-9) and 2 weeks (n = 6-9) and 10 weeks (n = 4-5) later. We quantified Oxt messenger RNA with quantitative polymerase chain reaction (n = 5-9). Intranasal OT was administered to naïve and stressed mice tested in social interaction and resident-intruder tests (n = 8-14). Acute exposure to a third episode of defeat increased OT/c-fos colocalizations in the paraventricular nucleus of both sexes. In the medioventral bed nucleus of the stria terminalis, defeat increased Oxt messenger RNA, total OT neurons, and OT/c-fos colocalizations in female mice but not male mice. Intranasal OT failed to reverse stress-induced social withdrawal in female mice and reduced social interaction behavior in female mice naïve to defeat. In contrast, intranasal OT increased social interaction in stressed male mice and reduced freezing in the resident-intruder test. Social defeat induces long-lasting increases in OT production and OT/c-fos cells in the medioventral bed nucleus of the stria terminalis of female mice but not male mice. Intranasal OT largely reversed the effects of stress on behavior in male mice, but effects were mixed in female mice. These results suggest that changes in OT-sensitive networks contribute to sex differences in behavioral responses to stress. Copyright © 2015 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved.

Cigarette smoke inhalation increases the alveolar bone loss caused by primary occlusal trauma in a rat model.

Science.gov (United States)

Campos, M L G; Corrêa, M G; Júnior, F H N; Casati, M Z; Sallum, E A; Sallum, A W

2014-04-01

Occlusal trauma (OT) and smoking are both factors that alter alveolar bone metabolism and therefore could synergistically act on alveolar bone loss. The aim of this experimental study was to evaluate the influence of short-term cigarette smoke inhalation (CSI) on inter-radicular alveolar bone loss promoted by primary OT in a rat model. Forty-eight animals were randomly assigned to one of three groups based on treatment type: OT + CSI (n = 16), animals were exposed to CSI three times per day, for 8 min per exposure, and they concomitantly received unilateral vertical augmentation creating an occlusal interference inducing experimental OT; OT (n = 16), animals received only unilateral vertical augmentation; negative control (NC; n = 16), animals maintained for equal periods to achieve periodontal baseline values of periodontal ligament dimension. Each group was divided into two subgroups (n = 8) based on treatment length: 7 or 14 d. After 7 d, the OT + CSI group exhibited significantly higher bone loss compared to the NC group (p = 0.0022). After 14 d, the OT (p < 0.0001) and OT + CSI (p < 0.0001) groups presented significantly higher bone loss compared to the NC group, and OT + CSI resulted in significantly higher bone loss than OT alone (p = 0.0241). The number of tartrate-resistant acid phosphatase-positive cells on the linear surface of the bone crest after 7 d was significantly higher in the OT + CSI group as compared to the NC and OT groups (p < 0.0001 and p = 0.0045, respectively) and remained significantly higher in the OT + CSI group after 14 d, compared to the OT group (p < 0.0001). Short-term CSI increases early bone loss in association with OT after 7 d, and this worsens in severity after 14 d of exposure. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Oxytocin and Estrogen Receptor β in the Brain: An Overview.

Science.gov (United States)

Acevedo-Rodriguez, Alexandra; Mani, Shaila K; Handa, Robert J

2015-01-01

Oxytocin (OT) is a neuropeptide synthesized primarily by neurons of the paraventricular and supraoptic nuclei of the hypothalamus. These neurons have axons that project into the posterior pituitary and release OT into the bloodstream to promote labor and lactation; however, OT neurons also project to other brain areas where it plays a role in numerous brain functions. OT binds to the widely expressed OT receptor (OTR), and, in doing so, it regulates homeostatic processes, social recognition, and fear conditioning. In addition to these functions, OT decreases neuroendocrine stress signaling and anxiety-related and depression-like behaviors. Steroid hormones differentially modulate stress responses and alter OTR expression. In particular, estrogen receptor β activation has been found to both reduce anxiety-related behaviors and increase OT peptide transcription, suggesting a role for OT in this estrogen receptor β-mediated anxiolytic effect. Further research is needed to identify modulators of OT signaling and the pathways utilized and to elucidate molecular mechanisms controlling OT expression to allow better therapeutic manipulations of this system in patient populations.

Ontogenesis of oxytocin pathways in the mammalian brain: late maturation and psychosocial disorders

Directory of Open Access Journals (Sweden)

Valery eGrinevich

2015-01-01

Full Text Available Oxytocin (OT, the main neuropeptide of sociality, is expressed in neurons exclusively localized in the hypothalamus. During the last decade, a plethora of neuroendocrine, metabolic, autonomic and behavioral effects of OT has been reported. In the urgency to find treatments to syndromes as invalidating as autism, many clinical trials have been launched in which OT is administered to patients, including adolescents and children. However, the impact of OT on the developing brain and in particular on the embryonic and early postnatal maturation of OT neurons, has been only poorly investigated. In the present review we summarize available (although limited literature on general features of ontogenetic transformation of the OT system, including determination, migration and differentiation of OT neurons. Next, we discuss trajectories of OT receptors (OTR in perinatal period. Furthermore, we provide evidence that early alterations, from birth, in the central OT system lead to severe neurodevelopmental diseases such as feeding deficit in infancy and severe defects in social behavior in adulthood, as described in Prader-Willi syndrome. Our review intends to propose a hypothesis about developmental dynamics of central OT pathways, which are essential for survival right after birth and for the acquisition of social skills later on. A better understanding of the embryonic and early postnatal maturation of the OT system may lead to better OT-based treatments in Prader-Willi syndrome and autism.

Operating theatre ventilation systems and microbial air contamination in total joint replacement surgery: results of the GISIO-ISChIA study.

Science.gov (United States)

Agodi, A; Auxilia, F; Barchitta, M; Cristina, M L; D'Alessandro, D; Mura, I; Nobile, M; Pasquarella, C

2015-07-01

Recent studies have shown a higher rate of surgical site infections in hip prosthesis implantation using unidirectional airflow ventilation compared with turbulent ventilation. However, these studies did not measure the air microbial quality of operating theatres (OTs), and assumed it to be compliant with the recommended standards for this ventilation technique. To evaluate airborne microbial contamination in OTs during hip and knee replacement surgery, and compare the findings with values recommended for joint replacement surgery. Air samplings were performed in 28 OTs supplied with unidirectional, turbulent and mixed airflow ventilation. Samples were collected using passive sampling to determine the index of microbial air contamination (IMA). Active sampling was also performed in some of the OTs. The average number of people in the OT and the number of door openings during the sampling period were recorded. In total, 1228 elective prosthesis procedures (60.1% hip and 39.9% knee) were included in this study. Of passive samplings performed during surgical activity in unidirectional airflow ventilation OTs (U-OTs) and mixed airflow OTs (M-OTs), 58.9% and 87.6% had IMA values >2, respectively. Of samplings performed during surgical activity in turbulent airflow OTs (T-OTs) and in turbulent airflow OTs with the surgical team wearing Steri-Shield Turbo Helmets (TH-OTs), 8.6% and 60% had IMA values ≤ 2, respectively. Positive correlation was found between IMA values and the number of people in the OT and the number of door openings (P systems always provide acceptable airborne bacterial counts. Copyright © 2015 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

The relationship between demand and need for orthodontic treatment in high school students in Bangkok.

Science.gov (United States)

Atisook, Pitraporn; Chuacharoen, Rattiya

2014-07-01

Orthodontic service is limited in Thailand and cannot meet the demand of the population. (1) To assess the need for orthodontic treatment (OT) using the Index of Orthodontic Treatment Need (IOTN) to analyze the relationship between demand and need for OT and (2) to compare the demand and need for OT between genders. A cross-sectional study was conducted on 450 students aged 12- to 14-years-old in three government high schools in Bangkok. A constructed questionnaire was used to assess demand for OT Clinical examination was done by two orthodontists to determine the needfor OT using the IOTN RESULTS: Most of the students (74.0%) wished to have OT while only one-third (37.5%) had severe need, and one-third (34.4%) had moderate need for OT as judge by the DHC of the IOTN. The AC of the IOTN indicated that most students (55.8%) had mild or no need for OT Females (79%) demanded OT more than males (66% p-value = 0.033) but the need was similar in both sexes. Most functional factors had strong relationships with the demand for OTexcept lower teeth bite on palate, but none was found to be associated with need for OT All of the aesthetic factors had strong relationships with demand for OT There were significant relationships with needs in five categories, 1) crooked, crowded, or spacing teeth, 2) worried when speaking or smiling, 3) had suggestedfor OT 4) breath smell and halitosis, and 5) wanted to put on braces to be like other people or for fashionable reasons. Most of the students requested OT but females had significantly higher demand for OT than males. Most of the samples needed to have OT The aesthetic factors that had strong relationships with the need for OT were 1) crooked, crowded, or spacing teeth, 2) worried when speaking or smiling, 3) had suggested for T07 4) breath smell and halitosis, and 5) wanted to put on braces to be like otherpeople orfor fashionable reasons.

Oxytocin Removes Estrous Female vs. Male Preference of Virgin Male Rats: Mediation of the Supraoptic Nucleus Via Olfactory Bulbs

Directory of Open Access Journals (Sweden)

Xiao-Yu Liu

2017-10-01

Full Text Available Social functions of oxytocin (OT have been explored extensively; however, relationship between the effect of intranasally applied OT (nasal OT on the social preference (SP and intracerebral actions of endogenous OT remains unclear. To resolve this question, we first observed effects of nasal OT on the SP of virgin young adult male rats toward unfamiliar virgin estrous female (EF vs. virgin male rats. The results showed that the test male rats exhibited significantly more times and longer duration accessing the female than the male, which were acutely eliminated by nasal OT. Then, we examined the approaches mediating nasal OT effects on the activity of potential brain targets in Western blots and found that nasal OT activated the olfactory bulbs (OBs and the supraoptic nucleus (SON, but not the piriform cortex, amygdala and hippocampus as shown by significant changes in the expression of c-Fos and/or phosphorylated extracellular signal-regulated protein kinase (pERK 1/2. Moreover, microinjection of TTX into the OBs blocked nasal OT-evoked increases in pERK1/2 levels as well as the molecular association between ERK1/2 and OT-neurophysin in the SON. Electrolytic lesions of the lateral olfactory tract did not significantly change the basal levels of pERK 1/2 in the SON; however, upon nasal OT, pERK 1/2 levels in the SON reduced significantly. Lastly, microinjection of L-aminoadipic acid (gliotoxin into the SON to reduce OT levels reduced the duration of the test male’s accessing the EF and blocked the nasal OT-evoked increase in the duration of test male’s accessing the male while significantly increasing pERK1/2 levels in the amygdala. These findings reveal for the first time that nasal OT acutely eliminates virgin males’ SP to EFs via the OB-SON route and that OT neurons could mediate the social effects of nasal OT by suppressing social phobia generated in the amygdala.

Oxytocin as a novel therapeutic option for type I diabetes and diabetic osteopathy.

Science.gov (United States)

Elabd, S K; Sabry, I; Mohasseb, M; Algendy, A

2014-04-01

The aim of the present study was to highlight the newly discovered metabolic role of oxytocin (OT) in the type I diabetic rats. Previous studies have demonstrated that OT has a beneficial role on bone physiology and therefore, the OT effect on the diabetic osteopathy will be assessed as well. Induction of the type I diabetes was carried out by an intraperitoneal injection of 60 mg/kg body weight of streptozotocin. The metabolic role of OT on diabetic rats after OT treatment with intramuscular injection of 40 µIU/kg body weight for 6 weeks was assessed. Histological and ultrastructural studies of rat pancreas samples, before and after the OT injection, were performed and compared with the obtained physiological results. Oxytocin treatment had positive metabolic effects in diabetic rats. This is based on the change in glucose metabolism, lipid profile, and insulin sensitivity in experimental animals. In addition, OT treatment showed histological regenerative changes of pancreatic islet cells of diabetic rats. Moreover, OT administration showed that it has an anabolic effect on the bone biology. The results suggest that activation of the oxytocin receptor (OTR) pathway by infusion of OT, OT analogs, or OT agonists may represent a promising approach for the treatment of diabetes and some of its complications, including diabetic osteopathy.

Salivary Oxytocin Concentrations in Males following Intranasal Administration of Oxytocin: A Double-Blind, Cross-Over Study.

Science.gov (United States)

Daughters, Katie; Manstead, Antony S R; Hubble, Kelly; Rees, Aled; Thapar, Anita; van Goozen, Stephanie H M

2015-01-01

The use of intranasal oxytocin (OT) in research has become increasingly important over the past decade. Although researchers have acknowledged a need for further investigation of the physiological effects of intranasal administration, few studies have actually done so. In the present double-blind cross-over study we investigated the longevity of a single 24 IU dose of intranasal OT measured in saliva in 40 healthy adult males. Salivary OT concentrations were significantly higher in the OT condition, compared to placebo. This significant difference lasted until the end of testing, approximately 108 minutes after administration, and peaked at 30 minutes. Results showed significant individual differences in response to intranasal OT administration. To our knowledge this is the largest and first all-male within-subjects design study to demonstrate the impact of intranasal OT on salivary OT concentrations. The results are consistent with previous research in suggesting that salivary OT is a valid matrix for OT measurement. The results also suggest that the post-administration 'wait-time' prior to starting experimental tasks could be reduced to 30 minutes, from the 45 minutes typically used, thereby enabling testing during peak OT concentrations. Further research is needed to ascertain whether OT concentrations after intranasal administration follow similar patterns in females, and different age groups.

Salivary Oxytocin Concentrations in Males following Intranasal Administration of Oxytocin: A Double-Blind, Cross-Over Study.

Directory of Open Access Journals (Sweden)

Katie Daughters

Full Text Available The use of intranasal oxytocin (OT in research has become increasingly important over the past decade. Although researchers have acknowledged a need for further investigation of the physiological effects of intranasal administration, few studies have actually done so. In the present double-blind cross-over study we investigated the longevity of a single 24 IU dose of intranasal OT measured in saliva in 40 healthy adult males. Salivary OT concentrations were significantly higher in the OT condition, compared to placebo. This significant difference lasted until the end of testing, approximately 108 minutes after administration, and peaked at 30 minutes. Results showed significant individual differences in response to intranasal OT administration. To our knowledge this is the largest and first all-male within-subjects design study to demonstrate the impact of intranasal OT on salivary OT concentrations. The results are consistent with previous research in suggesting that salivary OT is a valid matrix for OT measurement. The results also suggest that the post-administration 'wait-time' prior to starting experimental tasks could be reduced to 30 minutes, from the 45 minutes typically used, thereby enabling testing during peak OT concentrations. Further research is needed to ascertain whether OT concentrations after intranasal administration follow similar patterns in females, and different age groups.

Kuulus Roman Viktjuki Teater Tallinnas / Meelis Kapstas

Index Scriptorium Estoniae

Kapstas, Meelis, 1963-

2000-01-01

26. ja 27. mail Vene Kultuurikeskuses Roman Viktjuki Teatri külalisetendused. Mängitakse R. Viktjuki lavastust "Kellavärgiga apelsin" Anthony Burgessi romaani järgi ja Viktjuki versiooni Oscar Wilde'i kurbmängust "Salomé"

Kuninglik kingitus Sofiero / Meeli Müüripeal

Index Scriptorium Estoniae

Müüripeal, Meeli, 1963-

2005-01-01

Sofiero pargist, mille tulevane Rootsi kuningas Gustav VI Adolf ja kroonprintsess Margareta said 1905. a. pulmakingiks, mille nad tegid kauniks ja kuulsaks ning pärandasid Helsingborgi linnale. Margareta avaldatud aiaraamatutest. Juubelisuveks ilmus rootsi ja inglise keeles kogumik "Sofiero. Royal Residence and Glorius Garden". 18 ill

Tallinna evakueerimine 1944. a. septembris / Meelis Maripuu

Index Scriptorium Estoniae

Maripuu, Meelis

2008-01-01

Saksa vägede ja tsiviilisikute evakueerimisest Tallinnast 1944. aasta septembris. Tabel 1: Eesti mahajätmise plaani korrigeerimine taandumise käigus. Evakueeritud tsiviilisikute erinevatest gruppidest. Evakueerimise käigust. Evakueerimise tipp-päevaks kujunes 20. september. Tabel 2: Tallinnast 18.-22. septembril 1944 lahkunud Saksa laevad evakueeritutega

Kivirähk VAT Teatris / Meelis Kapstas

Index Scriptorium Estoniae

Kapstas, Meelis, 1963-

1999-01-01

VAT Teatri hooaja repertuaar. A. Kivirähki näidend "Sibulad ja šokolaad". Järgmise aasta jaanuaris saab teater oma statsionaarse lava - amfiteatri põhimõttel tehtud kammersaali Rahvusraamatukogus

Ainult kaks küsimust / Meelis Piller

Index Scriptorium Estoniae

Piller, Meelis

2011-01-01

Projekti autor 1. märtsist Tallinnas Eksperimentaalgaleriis ja CocaCola Plazas avatud vanade stereofotode näitusest "Linna-ruum 1900", stereofotograafia ajaloost lühidalt. Tallinna vaadetega stereofotode negatiivid on pärit Eesti Ajaloomuuseumist ja Eesti Filmiarhiivist. Kõige suurema stereofotode pärandi on jätnud fotograaf Jaan Riet

Elektriõnnetustest aastal 2005 / Meelis Kärt

Index Scriptorium Estoniae

Kärt, Meelis

2006-01-01

Tehnilise Järelevalve Inspektsiooni andmed 2005. a. toimunud elektriga seotud õnnetusjuhtumite kohta. Võrdlusandmed varasemate aastatega. Statistilised diagrammid. Vt. ka: Võrk, Rein. Meenutusi elektriõnnetuste ekspertiisidest

Reportaaž hilissuviselt Hämemaalt / Meelis Oidsalu

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Oidsalu, Meelis, 1978-

2009-01-01

Bertolt Brechti näidend, mis kirjutatud Hella Wuolijoe jutustuste ja näidendikavandi järgi "Härra Punttila ja tema sulane Matti". Lavastaja Kalju Komissarov, esietendus 19. juunil Ugala tiigi kaldal

Internetikunsti jätkuv tulemine / Meelis Kaldalu

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Kaldalu, Meelis

2004-01-01

Internetikunst (veebikunst, interaktiivne kunst, võrgukunst, netikunst, net.art) - loominguline tegevus, mis peab Internetti oma auditooriumiks. Manifestid. Internetikunst ja reaalsuse populaarsus, uus meedia. Internetiaadressid

Eeluurimiskohtunik kriminaalmenetluse seadustiku eelnõus / Meelis Eerik

Index Scriptorium Estoniae

Eerik, Meelis, 1976-

2000-01-01

Eeluurimiskohtuniku funktsioonidest uurimistoimingute sanktsioneerimisel, kriminaalmenetluse tagamisel, kohtueelses, väljaandmis- ja kohtumenetluses ning eeluurimiskohtuniku poolt tehtavatest lahenditest

Astmed ja hüpped / Meelis Friedenthal

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Friedenthal, Meelis, 1973-

2010-01-01

Dan Gordoni näidend "Vihmamees" (Rain Man) samanimelise filmi põhjal, filmistsenaariumi autorid Ronald Bass ja Barry Morrow, stsenaariumi aluseks Barry Morrow jutustus, lavastaja Georg Malvius. Esietendus 20. novembril Vanemuise väikeses majas

Nädalalõpuga kaks "Hamletit" / Meelis Kapstas

Index Scriptorium Estoniae

Kapstas, Meelis, 1963-

2000-01-01

Nädalavahetuse esietendustest : W. Gombrowiczi "Laulatus", lavastaja Mati Unt, esietendus 17. märtsil Vanemuises ; O. Muhhina "Tanja, Tanja", lavastaja Kalju Komissarov, esietendus 17. märtsil Rakvere Teatris ; M. Kundera "Jacques ja tema isand", lavastaja Ain Mäeots, esietendus 18. märtsil Vanemuises ; Luigi Lunari "Senaator Fox", lavastaja Tiit Palu, esietendus 18. märtsil Endla Teatris

The oncoprotein v-Myb activates transcription of Gremlin 2 during in vitro differentiation of the chicken neural crest to melanoblasts

Czech Academy of Sciences Publication Activity Database

Starostová, Michaela; Čermák, Vladimír; Dvořáková, Marta; Karafiát, Vít; Kosla, Jan; Dvořák, Michal

2014-01-01

Roč. 540, č. 1 (2014), s. 122-129 ISSN 0378-1119 R&D Projects: GA AV ČR KAN200520801 Institutional support: RVO:68378050 Keywords : Melanocyte development * Tamoxifen-inducible v-Myb * v-Myb-dependent genes * PRDC * KRT19 Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.138, year: 2014

Body temperature and cardiac changes induced by peripherally administered oxytocin, vasopressin and the non-peptide oxytocin receptor agonist WAY 267,464: a biotelemetry study in rats

Science.gov (United States)

Hicks, C; Ramos, L; Reekie, T; Misagh, G H; Narlawar, R; Kassiou, M; McGregor, I S

2014-01-01

Background and Purpose There is current interest in oxytocin (OT) as a possible therapeutic in psychiatric disorders. However, the usefulness of OT may be constrained by peripheral autonomic effects, which may involve an action at both OT and vasopressin V1A receptors. Here, we characterized the cardiovascular and thermoregulatory effects of OT, vasopressin (AVP) and the non-peptide OT receptor agonist WAY 267,464 in rats, and assessed the relative involvement of the OT and V1A receptors in these effects. Experimental Approach Biotelemetry in freely moving male Wistar rats was used to examine body temperature and heart rate after OT (0.01 – 1 mg kg−1; i.p.), AVP (0.001 – 0.1 mg kg−1; i.p.) or WAY 267,464 (10 and 100 mg kg−1; i.p.). The actions of the OT receptor antagonist Compound 25 (C25, 5 and 10 mg kg−1) and V1A receptor antagonist SR49059 (1 and 10 mg kg−1) were studied, as well as possible V1A receptor antagonist effects of WAY 267,464. Key Results OT and AVP dose-dependently reduced body temperature and heart rate. WAY 267,464 had similar, but more modest, effects. SR49059, but not C25, prevented the hypothermia and bradycardia induced by OT and AVP. WAY 267,464 (100 mg·kg−1) prevented the effects of OT, and to some extent AVP. Conclusions and Implications Peripherally administered OT and AVP have profound cardiovascular and thermoregulatory effects that appear to principally involve the V1A receptor rather than the OT receptor. Additionally, WAY 267,464 is not a simple OT receptor agonist, as it has functionally relevant V1A antagonist actions. PMID:24641248

Body temperature and cardiac changes induced by peripherally administered oxytocin, vasopressin and the non-peptide oxytocin receptor agonist WAY 267,464: a biotelemetry study in rats.

Science.gov (United States)

Hicks, C; Ramos, L; Reekie, T; Misagh, G H; Narlawar, R; Kassiou, M; McGregor, I S

2014-06-01

There is current interest in oxytocin (OT) as a possible therapeutic in psychiatric disorders. However, the usefulness of OT may be constrained by peripheral autonomic effects, which may involve an action at both OT and vasopressin V1A receptors. Here, we characterized the cardiovascular and thermoregulatory effects of OT, vasopressin (AVP) and the non-peptide OT receptor agonist WAY 267,464 in rats, and assessed the relative involvement of the OT and V1A receptors in these effects. Biotelemetry in freely moving male Wistar rats was used to examine body temperature and heart rate after OT (0.01 - 1 mg kg(-1); i.p.), AVP (0.001 - 0.1 mg kg(-1); i.p.) or WAY 267,464 (10 and 100 mg kg(-1); i.p.). The actions of the OT receptor antagonist Compound 25 (C25, 5 and 10 mg kg(-1)) and V1A receptor antagonist SR49059 (1 and 10 mg kg(-1)) were studied, as well as possible V1A receptor antagonist effects of WAY 267,464. OT and AVP dose-dependently reduced body temperature and heart rate. WAY 267,464 had similar, but more modest, effects. SR49059, but not C25, prevented the hypothermia and bradycardia induced by OT and AVP. WAY 267,464 (100 mg·kg(-1)) prevented the effects of OT, and to some extent AVP. Peripherally administered OT and AVP have profound cardiovascular and thermoregulatory effects that appear to principally involve the V1A receptor rather than the OT receptor. Additionally, WAY 267,464 is not a simple OT receptor agonist, as it has functionally relevant V1A antagonist actions. © 2014 The British Pharmacological Society.

Puksoo auhind 2007/2008 / Tiiu Reimo

Index Scriptorium Estoniae

Reimo, Tiiu, 1953-

2009-01-01

23. jaanuaril 2009 Tartu Ülikooli Raamatukogus peetud Friedrich Puksoo 119. sünniaastapäevale pühendatud ettekandepäeval "Raamatust, raamatukogust, raamatukoguhoidjast" kuulutati välja järjekordsed Puksoo auhinna laureaadid. TÜR-i ja ERÜ poolt välja antava auhinna pälvis TÜR-i teaduri, filosoofiadoktor Meelis Friedenthali dissertatsioon "Tallinna Linnaarhiivi Tractatus moralis de oculo", tõsteti esile Jürgen Beyeri ja Larissa Petina tööd

Human ovolution and plasma oxytocin

International Nuclear Information System (INIS)

Kumaresan, Perianna; Kumaresan, Malathi; Hossini, Mahmood; Arellano, Carolina; Vasicka, Alois

1983-01-01

Plasma oxytocin (OT) concentrations were measured by radioimmunoassay (RIA) method without extracting plasma in 11 normal menstruating women. Mean plasma OT level began to increase steadily from the 7th day of the menstrual cycle and this level rose up to 20+-5 μU/ml (Mean+-S.E.) on the 10th day of the cycle. OT level declined to 13+-6 μU/ml on the day of the LH peak and continuously declined for another 2 days - then rose. The OT level was higher during the follicular phase than during the luteal phase. In 1 individual OT measured in 2 cycles a year apart showed the highest level of OT coincided with LH and FSH peak and abruptly declined. When there was the highest level of progesterone, the OT level was measurable 1 out of 11 cycles. From this study, we conclude that OT may have a role in human ovulation either synergistically or alone with other ovulatory mechanisms and ovarian estradiol and progesterone control the secretion of OT and also suggests that OT may play some role in the regulation of the luteolysis and the menstrual cycle in women. (author)

Oxytocin facilitates fidelity in well-established marmoset pairs by reducing sociosexual behavior toward opposite-sex strangers.

Science.gov (United States)

Cavanaugh, Jon; Mustoe, Aaryn C; Taylor, Jack H; French, Jeffrey A

2014-11-01

Behavioral strategies that facilitate the maintenance of social bonds are critical for the preservation of high-quality social relationships. Central oxytocin (OT) activity modulates the behavioral features of socially monogamous relationships in a number of mammalian species (including marmoset monkeys), and plays a vital role in the behavioral maintenance of long-term social relationships. Two distinct variants of OT have been identified in some New World primates (including marmosets; Lee et al., 2011). The marmoset variant of the oxytocin ligand (Pro(8)-OT) is structurally distinct from the consensus mammalian variant of the oxytocin ligand (Leu(8)-OT), due to a proline substitution at the 8th amino-acid position. The goal of the present study was to determine if treating marmosets with Pro(8)-OT, relative to treatments with Leu(8)-OT, control saline, or an OT antagonist, had modulatory effects on the behavioral maintenance of long-term social relationships in marmosets. Treatment with the Pro(8) variant, but not the Leu(8) variant, of OT facilitated fidelity with a long-term partner by reducing time spent in close proximity with an opposite-sex stranger. However, this facilitative effect of Pro(8)-OT on proximity behavior manifested itself differently in male and female marmosets, such that females preferred to interact socially with their partner rather than a stranger when treated with Pro(8)-OT, while males spent less time in close proximity with both their partner and a stranger when treated with Pro(8)-OT. Furthermore, treatment with Pro(8)-OT, but not Leu(8)-OT, significantly delayed the expression of sexual solicitation behavior toward an opposite-sex stranger in both male and female marmosets, but had no effect on sociosexual behavior directed toward a long-term partner. These results suggest that the OT system is highly involved in reducing fidelity-threatening behaviors in well-established marmoset pairs, and that the effects were only produced by

The organizational effects of oxytocin on the central expression of estrogen receptor α and oxytocin in adulthood

Directory of Open Access Journals (Sweden)

Papademetriou Eros

2007-09-01

Full Text Available Abstract Background Previous studies have demonstrated that neonatal manipulation of oxytocin (OT has effects on the expression of estrogen receptor α (ERα and the central production of oxytocin observed in juveniles (at weaning, 21 days of age. The goal of this study was to determine whether the effects of neonatal manipulation of OT last into adulthood, and if the effects differ from those observed during the early postnatal period. On the first day of life, prairie voles (Microtus ochrogaster received one of three doses of OT (High, 3 μg; Med, 0.3 μg; Low, 0.03 μg, an OT antagonist, or isotonic saline. Another group was handled, but not injected. Then as adults, brains were collected, sectioned, and stained for ERα or OT using immunocytochemistry. Results In females, treatment with OT increased the expression of ERα immunoreactivity in the ventral lateral septum (0.03 μg and the ventromedial nucleus of the hypothalamus and central amygdala (0.3 μg. In males, OT antagonist increased ERα expression in the bed nucleus of the stria terminalis. There was no apparent effect of OT on the number of cells producing OT in the paraventricular nucleus of the hypothalamus. Conclusion The current results suggest that neonatal manipulation of OT has long-term organizational effects on the expression of ERα in both males and females. The lack of effect on OT neurons in the paraventricular nucleus suggests that some developmental effects of OT previously observed in weanlings do not persist into adulthood. Developmental effects of OT on ERα patterns were sexually dimorphic, dose-dependent, and site-specific.

Conditioned same-sex partner preference in male rats is facilitated by oxytocin and dopamine: effect on sexually dimorphic brain nuclei.

Science.gov (United States)

Triana-Del Rio, Rodrigo; Tecamachaltzi-Silvarán, Miriam B; Díaz-Estrada, Victor X; Herrera-Covarrubias, Deissy; Corona-Morales, Aleph A; Pfaus, James G; Coria-Avila, Genaro A

2015-04-15

Conditioned same-sex partner preference can develop in male rats that undergo cohabitation under the effects of quinpirole (QNP, D2 agonist). Herein, we assessed the development of conditioned same-sex social/sexual preference in males that received either nothing, saline, QNP, oxytocin (OT), or QNP+OT during cohabitation with another male (+) or single-caged (-). This resulted in the following groups: (1) Intact-, (2) Saline+, (3) QNP-, (4) OT-, (5) QNP+, (6) OT+ and (7) QNP/OT+. Cohabitation occurred during 24h in a clean cage with a male partner that bore almond scent on the back as conditioned stimulus. This was repeated every 4 days for a total of three trials. Social and sexual preference were assessed four days after the last conditioning trial in a drug-free test in which experimental males chose between the scented familiar male and a novel sexually receptive female. Results showed that males from groups Intact-, Saline+, QNP- and OT- displayed a clear preference for the female (opposite-sex), whereas groups QNP+, OT+ and QNP/OT+ displayed socio/sexual preference for the male partner (same-sex). In Experiment 2, the brains were processed for Nissl dye and the area size of two sexually dimorphic nuclei (SDN-POA and SON) was compared between groups. Males from groups OT-, OT+ and QNP/OT+ expressed a smaller SDN-POA and groups QNP+ and QNP/OT+ expressed a larger SON. Accordingly, conditioned same-sex social/sexual partner preference can develop during cohabitation under enhanced D2 or OT activity but such preference does not depend on the area size of those sexually dimorphic nuclei. Copyright © 2015 Elsevier B.V. All rights reserved.

Detection of Tropical Overshooting Cloud Tops Using Himawari-8 Imagery

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Miae Kim

2017-07-01

Full Text Available Abstract: Overshooting convective cloud Top (OT-accompanied clouds can cause severe weather conditions, such as lightning, strong winds, and heavy rainfall. The distribution and behavior of OTs can affect regional and global climate systems. In this paper, we propose a new approach for OT detection by using machine learning methods with multiple infrared images and their derived features. Himawari-8 satellite images were used as the main input data, and binary detection (OT or nonOT with class probability was the output of the machine learning models. Three machine learning techniques—random forest (RF, extremely randomized trees (ERT, and logistic regression (LR—were used to develop OT classification models to distinguish OT from non-OT. The hindcast validation over the Southeast Asia and West Pacific regions showed that RF performed best, resulting in a mean probabilities of detection (POD of 77.06% and a mean false alarm ratio (FAR of 36.13%. Brightness temperature at 11.2 μm (Tb11 and its standard deviation (STD in a 3 × 3 window size were identified as the most contributing variables for discriminating OT and nonOT classes. The proposed machine learning-based OT detection algorithms produced promising results comparable to or even better than the existing approaches, which are the infrared window (IRW-texture and water vapor (WV minus IRW brightness temperature difference (BTD methods.

CSF and blood oxytocin concentration changes following intranasal delivery in macaque.

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Olga Dal Monte

Full Text Available Oxytocin (OT in the central nervous system (CNS influences social cognition and behavior, making it a candidate for treating clinical disorders such as schizophrenia and autism. Intranasal administration has been proposed as a possible route of delivery to the CNS for molecules like OT. While intranasal administration of OT influences social cognition and behavior, it is not well established whether this is an effective means for delivering OT to CNS targets. We administered OT or its vehicle (saline to 15 primates (Macaca mulatta, using either intranasal spray or a nebulizer, and measured OT concentration changes in the cerebral spinal fluid (CSF and in blood. All subjects received both delivery methods and both drug conditions. Baseline samples of blood and CSF were taken immediately before drug administration. Blood was collected every 10 minutes after administration for 40 minutes and CSF was collected once post-delivery, at the 40 minutes time point. We found that intranasal administration of exogenous OT increased concentrations in both CSF and plasma compared to saline. Both delivery methods resulted in similar elevations of OT concentration in CSF, while the changes in plasma OT concentration were greater after nasal spray compared to nebulizer. In conclusion our study provides evidence that both nebulizer and nasal spray OT administration can elevate CSF OT levels.

The Mediating Role of Organizational Trust on The Effects of Servant Leader ship and Organizational Justice on Organizational Identification

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Fikret Ateş

2015-09-01

Full Text Available The aim of this study determined to research whether was affected Organizational Identification (OI by Servant Leadership (SL, Organizational Justice (OJ, Organizational Trust (OT and, how was affected OI by SL and OJ and, whether there is an indirecteffect of OT on the relations of OI-SL and OI-OJ. For these aims, the questionnaire was developed with Dennis and Bocerne (2006's SL scale, Colquitt (2001's OJ scale, Mael and Ashforth (1992's OI scale, Bromiley and Cummings (1996’s OT scale. The validity of scales authenticated with multi-factor structure for SL, OJ, and OT, with one factor structure for OI. The reliability of scales were calculated as Cronbach's alpha (α values (SL=0.95; OJ=0.93, OT=0.76, OI=0.87. According to the hypothesis tests which done by Structural Equation Models; has that there are direct positive effects between SL, OJ, OT and OI or between SL, OJ and OT; finally has determined that there are positive and partial indirect effects between OT and SL-OI or between OT andOJ-OI.

Awareness and Use of Electronic Health Records in Entry-Level Occupational Therapy and Occupational Therapy Assistant Curricula

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Louis F. Dmytryk

2017-03-01

Full Text Available The Accreditation Council for Occupational Therapy Education (ACOTE requires programs to instruct entrylevel occupational therapy (OT and occupational therapy assistant (OTA students in technology that may include electronic documentation systems, distance communication, virtual environments, and telehealth (standard B1.8. At this time, there are no publications describing if and how electronic health record (EHR instruction is implemented in entry-level OT and OTA programs. The purpose of this study is to investigate awareness and use of EHRs in entry-level OT and OTA curricula. Respondents from 76 nationally accredited entry-level programs (two OT doctoral, 24 OT masters, two OT combined bachelors/masters, and 48 OTA completed a survey. The findings showed inconsistent and incomplete EHR instruction in entry-level OT and OTA education. This study provides a baseline for investigating best practices in EHR education for entrylevel OT and OTA students

Resenha de 1976: Movimento Black Rio, de Luiz Felipe de Lima Peixoto e Zé Otávio Sabadelhe (Rio de Janeiro: José Olympio, 2016

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Carlos Palombini

2017-08-01

Full Text Available Lançado em 10 de novembro de 2016, o livro 1976: Movimento Black Rio, dos jornalistas Luiz Felipe de Lima Peixoto e Zé Otávio Sabadelhe, celebra os quarenta anos da manchete de Lena Frias, Black Rio: o orgulho (importado de ser negro no Brasil, publicada em 17 de julho de 1976 no Caderno B do Jornal do Brasil. Em 28 reportagens, os autores recorrem a entrevistas e material previamente publicado para contar a história da cultura de bailes que, alimentados por soul, funk e disco afro-norte-americanos, espalharam-se pela Zona Norte carioca nos anos 1970, bem como a história do conjunto das apropriações desses gêneros por Tim Maia, Toni Tornado, Hyldon, Carlos Dafé, Cassiano, Gerson King Combo, Dom Salvador e Abolição, União Black, e Banda Black Rio. O livro tenciona constituir “uma contribuição à construção discursiva de uma memória social positiva da população negra brasileira” e oferece elementos para um trabalho necessário de revisão historiográfica que transcende o âmbito do tema tratado, apesar de erros fatuais que poderiam ter sido corrigidos por cruzamento de dados.

Central oxytocin and food intake: focus on macronutrient-driven reward

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Anica eKlockars

2015-04-01

Full Text Available Centrally acting oxytocin (OT is known to terminate food consumption in response to excessive stomach distension, increase in salt loading and presence of toxins. Hypothalamic-hindbrain OT pathways facilitate these aspects of OT-induced hypophagia. However, recent discoveries have implicated OT in modifications of feeding via reward circuits: OT has been found to differentially affect consumption of individual macronutrients in choice and no-choice paradigms. In this mini-review, we focus on presenting and interpreting evidence that defines OT as a key component of mechanisms that reduce eating for pleasure and shape macronutrient preferences. We also provide remarks on challenges in integrating the knowledge on physiological and pathophysiological states in which both OT activity and macronutrient preferences are affected.

The role of oxytocin in cardiovascular regulation

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J. Gutkowska

2014-03-01

Full Text Available Studies of body volume expansion have indicated that lesions of the anteroventral third ventricle and median eminence block the release of atrial natriuretic peptide (ANP into the circulation. Detailed analysis of the lesions showed that activation of oxytocin (OT-ergic neurons is responsible for ANP release, and it has become clear that activation of neuronal circuitry elicits OT secretion into the circulation, activating atrial OT receptors and ANP release from the heart. Subsequently, we have uncovered the entire functional OT system in the rat and the human heart. An abundance of OT has been observed in the early development of the fetal heart, and the capacity of OT to generate cardiomyocytes (CMs has been demonstrated in various types of stem cells. OT treatment of mesenchymal stem cells stimulates paracrine factors beneficial for cardioprotection. Cardiovascular actions of OT include: i lowering blood pressure, ii negative inotropic and chronotropic effects, iii parasympathetic neuromodulation, iv vasodilatation, v anti-inflammatory activity, vi antioxidant activity, and vii metabolic effects. OT actions are mediated by nitric oxide and ANP. The beneficial actions of OT may include the increase in glucose uptake by CMs and stem cells, reduction in CM hypertrophy, oxidative stress, and mitochondrial protection of several cell types. In experimentally induced myocardial infarction in rats, continuous in vivo OT delivery improves cardiac healing and cardiac work, reduces inflammation, and stimulates angiogenesis. Because OT plays anti-inflammatory and cardioprotective roles and improves vascular and metabolic functions, it demonstrates potential for therapeutic use in various pathologic conditions.

Manipulation of the oxytocin system alters social behavior and attraction in pair-bonding primates, Callithrix penicillata.

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Smith, Adam S; Agmo, Anders; Birnie, Andrew K; French, Jeffrey A

2010-02-01

The establishment and maintenance of stable, long-term male-female relationships, or pair-bonds, are marked by high levels of mutual attraction, selective preference for the partner, and high rates of sociosexual behavior. Central oxytocin (OT) affects social preference and partner-directed social behavior in rodents, but the role of this neuropeptide has yet to be studied in heterosexual primate relationships. The present study evaluated whether the OT system plays a role in the dynamics of social behavior and partner preference during the first 3 weeks of cohabitation in male and female marmosets, Callithrix penicillata. OT activity was stimulated by intranasal administration of OT, and inhibited by oral administration of a non-peptide OT-receptor antagonist (L-368,899; Merck). Social behavior throughout the pairing varied as a function of OT treatment. Compared to controls, marmosets initiated huddling with their social partner more often after OT treatments but reduced proximity and huddling after OT antagonist treatments. OT antagonist treatment also eliminated food sharing between partners. During the 24-h preference test, all marmosets interacted more with an opposite-sex stranger than with the partner. By the third-week preference test, marmosets interacted with the partner and stranger equally with the exception that intranasal-OT treatments facilitated initial partner-seeking behavior over initial contact with the stranger. Our findings demonstrate that pharmacological manipulations of OT activity alter partner-directed social behavior during pair interactions, suggesting that central OT may facilitate the process of pair-bond formation and social relationships in marmoset monkeys. Published by Elsevier Inc.

The role of oxytocin in cardiovascular regulation

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Gutkowska, J.; Jankowski, M. [University of Montreal, CHUM Research Centre, Faculty of Medicine, Department of Medicine, Laboratory of Cardiovascular Biochemistry, Montreal, Quebec, Canada, Laboratory of Cardiovascular Biochemistry, Department of Medicine, Faculty of Medicine, University of Montreal, CHUM Research Centre, Montreal, Quebec (Canada); Antunes-Rodrigues, J. [Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Fisiologia, Ribeirão Preto, SP, Brasil, Departamento de Fisiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil)

2014-03-03

Studies of body volume expansion have indicated that lesions of the anteroventral third ventricle and median eminence block the release of atrial natriuretic peptide (ANP) into the circulation. Detailed analysis of the lesions showed that activation of oxytocin (OT)-ergic neurons is responsible for ANP release, and it has become clear that activation of neuronal circuitry elicits OT secretion into the circulation, activating atrial OT receptors and ANP release from the heart. Subsequently, we have uncovered the entire functional OT system in the rat and the human heart. An abundance of OT has been observed in the early development of the fetal heart, and the capacity of OT to generate cardiomyocytes (CMs) has been demonstrated in various types of stem cells. OT treatment of mesenchymal stem cells stimulates paracrine factors beneficial for cardioprotection. Cardiovascular actions of OT include: i) lowering blood pressure, ii) negative inotropic and chronotropic effects, iii) parasympathetic neuromodulation, iv) vasodilatation, v) anti-inflammatory activity, vi) antioxidant activity, and vii) metabolic effects. OT actions are mediated by nitric oxide and ANP. The beneficial actions of OT may include the increase in glucose uptake by CMs and stem cells, reduction in CM hypertrophy, oxidative stress, and mitochondrial protection of several cell types. In experimentally induced myocardial infarction in rats, continuous in vivo OT delivery improves cardiac healing and cardiac work, reduces inflammation, and stimulates angiogenesis. Because OT plays anti-inflammatory and cardioprotective roles and improves vascular and metabolic functions, it demonstrates potential for therapeutic use in various pathologic conditions.

The role of oxytocin in cardiovascular regulation

International Nuclear Information System (INIS)

Gutkowska, J.; Jankowski, M.; Antunes-Rodrigues, J.

2014-01-01

Studies of body volume expansion have indicated that lesions of the anteroventral third ventricle and median eminence block the release of atrial natriuretic peptide (ANP) into the circulation. Detailed analysis of the lesions showed that activation of oxytocin (OT)-ergic neurons is responsible for ANP release, and it has become clear that activation of neuronal circuitry elicits OT secretion into the circulation, activating atrial OT receptors and ANP release from the heart. Subsequently, we have uncovered the entire functional OT system in the rat and the human heart. An abundance of OT has been observed in the early development of the fetal heart, and the capacity of OT to generate cardiomyocytes (CMs) has been demonstrated in various types of stem cells. OT treatment of mesenchymal stem cells stimulates paracrine factors beneficial for cardioprotection. Cardiovascular actions of OT include: i) lowering blood pressure, ii) negative inotropic and chronotropic effects, iii) parasympathetic neuromodulation, iv) vasodilatation, v) anti-inflammatory activity, vi) antioxidant activity, and vii) metabolic effects. OT actions are mediated by nitric oxide and ANP. The beneficial actions of OT may include the increase in glucose uptake by CMs and stem cells, reduction in CM hypertrophy, oxidative stress, and mitochondrial protection of several cell types. In experimentally induced myocardial infarction in rats, continuous in vivo OT delivery improves cardiac healing and cardiac work, reduces inflammation, and stimulates angiogenesis. Because OT plays anti-inflammatory and cardioprotective roles and improves vascular and metabolic functions, it demonstrates potential for therapeutic use in various pathologic conditions

Low-dose oxytocin delivered intranasally with Breath Powered device affects social-cognitive behavior: a randomized four-way crossover trial with nasal cavity dimension assessment.

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Quintana, D S; Westlye, L T; Rustan, Ø G; Tesli, N; Poppy, C L; Smevik, H; Tesli, M; Røine, M; Mahmoud, R A; Smerud, K T; Djupesland, P G; Andreassen, O A

2015-07-14

Despite the promise of intranasal oxytocin (OT) for modulating social behavior, recent work has provided mixed results. This may relate to suboptimal drug deposition achieved with conventional nasal sprays, inter-individual differences in nasal physiology and a poor understanding of how intranasal OT is delivered to the brain in humans. Delivering OT using a novel 'Breath Powered' nasal device previously shown to enhance deposition in intranasal sites targeted for nose-to-brain transport, we evaluated dose-dependent effects on social cognition, compared response with intravenous (IV) administration of OT, and assessed nasal cavity dimensions using acoustic rhinometry. We adopted a randomized, double-blind, double-dummy, crossover design, with 16 healthy male adults completing four single-dose treatments (intranasal 8 IU (international units) or 24 IU OT, 1 IU OT IV and placebo). The primary outcome was social cognition measured by emotional ratings of facial images. Secondary outcomes included the pharmacokinetics of OT, vasopressin and cortisol in blood and the association between nasal cavity dimensions and emotional ratings. Despite the fact that all the treatments produced similar plasma OT increases compared with placebo, there was a main effect of treatment on anger ratings of emotionally ambiguous faces. Pairwise comparisons revealed decreased ratings after 8 IU OT in comparison to both placebo and 24 IU OT. In addition, there was an inverse relationship between nasal valve dimensions and anger ratings of ambiguous faces after 8-IU OT treatment. These findings provide support for a direct nose-to-brain effect, independent of blood absorption, of low-dose OT delivered from a Breath Powered device.

Opryszczkowe zapalenie mózgu przebiegające w postaci szybko narastającego zespołu otępiennego u chorej na reumatoidalne zapalenie stawów, leczonej infliksymabem

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Justyna Swarowska-Knap

2011-06-01

Full Text Available Przedstawiono przypadek 53-letniej chorej na reumatoidalnezapalenie stawów (RZS o ciężkim, opornym na standardoweleczenie przebiegu, leczonej wieloma lekami modyfikującymi przebiegchoroby (LMPCh oraz glikokortykosteroidami, u którejw trakcie leczenia antycytokinowego (infliksymabem wystąpiłyobjawy szybko narastającego zespołu otępiennego. Ze względu nacoraz powszechniejsze zastosowanie leków antycytokinowych nietylko w dziedzinie reumatologii, coraz częściej w literaturze światowejpojawiają się doniesienia o związanych z ich stosowaniemdziałaniach niepożądanych, w tym powikłaniach infekcyjnychi zaburzeniach ze strony ośrodkowego układu nerwowego. Zaburzenianeurologiczne obserwowano w trakcie leczenia wszystkimiinhibitorami czynnika martwicy nowotworów α (tumour necrosisfactor α – TNF-α. Wśród nich występowały: parestezje (65% chorych,zaburzenia widzenia związane z zapaleniem nerwu wzrokowego(40% chorych i splątanie (25% chorych. Ponadto, wgdanych literaturowych, u chorych na RZS leczonych immunosupresyjnieistnieje większe niż w populacji ogólnej ryzyko poważnychinfekcji oraz reaktywacji latentnych infekcji wirusowych,w tym infekcji wirusami z rodziny Herpesviridae i wirusem JC. Ze względu na możliwy związek szybko ujawniających się zaburzeńneurologicznych z leczeniem infliksymabem wysunięto u pacjentkipodejrzenie postępującej wieloogniskowej leukoencefalopatii(progressive multifocal leukoencephalopathy – PML, będącej wynikiemaktywacji utajonej infekcji wirusem JC. W wykonanym badaniuMRI mózgowia stwierdzono obecność hiperintensywnychzmian zlokalizowanych w istocie białej oraz szarej płatów czołowychi skroniowych, które nie dawały efektu masy (ryc. 1. Lokalizacjazmian w płatach skroniowych była typowa dla opryszczkowegozapalenia mózgu (herpes simplex encephalitis – HSE, aleobecnie wiadomo, że podobne zmiany mogą także występowaćw PML. Obie neuroinfekcje mogą przebiega

Orthodontic treatment for disabled children: a survey of parents' attitudes and overall satisfaction.

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Abeleira, María Teresa; Pazos, Elisabeth; Ramos, Isabel; Outumuro, Mercedes; Limeres, Jacobo; Seoane-Romero, Juan; Diniz, Marcio; Diz, Pedro

2014-08-05

Many patients with disability require orthodontic treatment (OT) to achieve adequate oral function and aesthetic appearance. The cooperation of disabled patients and of their parents is central to the success of OT, as treatment can involve ethical dilemmas. The aim of this study was to analyze the motivation, expectations and overall satisfaction with OT among parents of patients with disabilities. The parents of 60 disabled Spanish children with physical, mental and/or sensory impairment undergoing OT were surveyed on attitudes to OT and level of satisfaction with the outcomes. The survey consisted of 23 questions in 4 sections: attitude and adaptation, benefits, adverse effects, and level of satisfaction after completion of OT. A control group formed of the parents of 60 healthy children undergoing OT at the same institution were also surveyed. Parents of disabled children undergoing OT showed a high level of motivation and they are willing to collaborate in oral hygiene procedures. Adaptation to the removable appliances was poorer in disabled children but adaptation to fixed appliances was excellent. OT can provide a marked improvement in quality of life, social relationships and oral functionality in disabled children. Among parents of disabled children undergoing OT, the perceived level of overall satisfaction was very high and expectations were often exceeded.

Comprehensive, blinded assessment of balance in orthostatic tremor.

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Bhatti, Danish; Thompson, Rebecca; Xia, Yiwen; Hellman, Amy; Schmaderer, Lorene; Suing, Katie; McKune, Jennifer; Penke, Cynthia; Iske, Regan; Roeder, Bobbi Jo; Siu, Ka-Chun; Bertoni, John M; Torres-Russotto, Diego

2018-02-01

Orthostatic Tremor (OT) is a movement disorder characterized by a sensation of unsteadiness and tremors in the 13-18 Hz range present upon standing. The pathophysiology of OT is not well understood but there is a relationship between the sensation of instability and leg tremors. Despite the sensation of unsteadiness, OT patients do not fall often and balance in OT has not been formally assessed. We present a prospective blinded study comparing balance assessment in patients with OT versus healthy controls. We prospectively enrolled 34 surface Electromyography (EMG)-confirmed primary OT subjects and 21 healthy controls. Participants underwent evaluations of balance by blinded physical therapists (PT) with standardized, validated, commonly used balance scales and tasks. OT subjects were mostly female (30/34, 88%) and controls were majority males (13/20, 65%). The average age of OT subjects was 68.5 years (range 54-87) and for controls was 69.4 (range 32-86). The average duration of OT symptoms was 18 years. OT subjects did significantly worse on all the balance scales and on most balance tasks including Berg Balance Scale, Functional Gait Assessment, Dynamic Gait Index, Unipedal Stance Test, Functional Reach Test and pull test. Gait speed and five times sit to stand were normal in OT. Common validated balance scales are significantly abnormal in primary OT. Despite the objective finding of impaired balance, OT patients do not commonly have falls. The reported sensation of unsteadiness in this patient population seems to be out of proportion to the number of actual falls. Further studies are needed to determine which components of commonly used balance scales are affected by a sensation of unsteadiness and fear of falling. Copyright © 2017 Elsevier Ltd. All rights reserved.

Association of Oxytocin and Parental Prefrontal Activation during Reunion with Infant: A Functional Near-Infrared Spectroscopy Study

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Jun Ito

2017-12-01

Full Text Available Although previous studies have revealed the role of oxytocin (OT in parental behavior, the role of OT has not been investigated through the direct assessment of prefrontal brain activation during parenting. By using functional near-infrared spectroscopy, we aimed to show the relationship between parental [maternal (N = 15 and paternal (N = 21] OT levels and the activation of the prefrontal cortex (PFC, while holding their infants after separation. Baseline OT levels were measured in the subjects’ saliva samples before the experiment. Prefrontal brain activation was assessed in participants sitting alone on a chair (i.e., separation from their infant for 120 s and during the target period (i.e., holding their infant for 45 s, which was done in triplicate. The oxygen hemoglobin (oxy-Hb dissociation curve significantly increased in 9 out of 22 channels on the PFC when maternal and paternal samples were combined. However, only the fathers showed a correlation between salivary OT and oxy-Hb signal. Furthermore, while holding their infants, high-OT fathers showed left hemispheric dominance compared to low-OT fathers, while high-OT mothers showed right hemispheric dominance compared to low-OT mothers. This study showed that fathers with high-OT levels showed neural activation with left hemispheric dominance, while holding their infants, suggesting that increase of OT level might activate paternal PFC related to parenting behavior, although the same is not true for mothers.

The acute effects of intranasal oxytocin administration on endocrine and sexual function in males.

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Burri, Andrea; Heinrichs, Markus; Schedlowski, Manfred; Kruger, Tillmann H C

2008-06-01

The role of the neuropeptide oxytocin (OT) ranges from the modulation of neuroendocrine physiological effects to the establishment of complex social and bonding behaviours. Experimental studies in animals, as well as case reports in humans, suggest that OT affects different aspects of sexual behaviour and has predominantly facilitating properties for sexual appetence and performance. Using a previously established experimental paradigm of sexual arousal and masturbation-induced orgasm, this study investigated the acute effects of intranasal OT application (24I.U.) on endocrine parameters and measures of sexual appetence and function in healthy men (n=10). In a double-blind, placebo-controlled, balanced cross-over design, sexual arousal, and orgasm were induced by an erotic film and masturbation. In addition to the continuous recording of endocrine (OT, cortisol, prolactin, epinephrine, norepinephrine) and cardiovascular data (heart rate), parameters of appetitive, consummatory, and refractory sexual behaviour were assessed using the acute sexual experience scale (ASES). OT plasma levels were significantly elevated after intranasal OT throughout the whole experiment (>60 min). In addition, OT treatment induced significantly higher increases in epinephrine plasma levels during sexual activity without affecting cortisol levels, prolactin levels or heart rate. OT treatment did not alter appetitive, consummatory, and refractory sexual behaviour according to the ASES. However, when subjects were asked about their subjective perception of whether OT or placebo had been applied, eight out of 10 subjects in the OT group answered correctly, thus pointing to an altered perception of arousal. In conclusion, intranasally administered OT leads to a marked increase in OT plasma levels together with increased secretion of catecholamines when subjects are engaged in sexual activity in a laboratory setting. As the effects of OT on sexual behaviour were equivocal, future studies

Distinct BOLD activation profiles following central and peripheral oxytocin administration in awake rats

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Craig F Ferris

2015-09-01

Full Text Available A growing body of literature has suggested that intranasal oxytocin (OT or other systemic routes of administration can alter prosocial behavior, presumably by directly activating OT sensitive neural circuits in the brain. Yet there is no clear evidence that OT given peripherally can cross the blood-brain-barrier at levels sufficient to engage the OT receptor. To address this issue we examined changes in blood oxygen level dependent (BOLD signal intensity in response to peripheral OT injections (0.1, 0.5 or 2.5 mg/kg during functional magnetic resonance (fMRI in awake rats imaged at 7.0 tesla. These data were compared to OT (1ug/5 µl given directly to the brain via the lateral cerebroventricle. Using a 3D annotated MRI atlas of the rat brain segmented into 171 brain areas and computational analysis we reconstructed the distributed integrated neural circuits identified with BOLD fMRI following central and peripheral OT. Both routes of administration caused significant changes in BOLD signal within the first 10 min of administration. As expected, central OT activated a majority of brain areas known to express a high density of OT receptors e.g., lateral septum, subiculum, shell of the accumbens, bed nucleus of the stria terminalis. This profile of activation was not matched by peripheral OT. The change in BOLD signal to peripheral OT did not show any discernible dose-response. Interestingly, peripheral OT affected all subdivisions of the olfactory bulb, in addition to the cerebellum and several brainstem areas relevant to the autonomic nervous system, including the solitary tract nucleus. The results from this imaging study do not support a direct central action of peripheral OT on the brain. Instead, the patterns of brain activity suggest that peripheral OT may interact at the level of the olfactory bulb and through sensory afferents from the autonomic nervous system to influence brain activity.

Oxytocin Acting in the Nucleus Accumbens Core Decreases Food Intake.

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Herisson, F M; Waas, J R; Fredriksson, R; Schiöth, H B; Levine, A S; Olszewski, P K

2016-04-01

Central oxytocin (OT) promotes feeding termination in response to homeostatic challenges, such as excessive stomach distension, salt loading and toxicity. OT has also been proposed to affect feeding reward by decreasing the consumption of palatable carbohydrates and sweet tastants. Because the OT receptor (OTR) is expressed in the nucleus accumbens core (AcbC) and shell (AcbSh), a site regulating diverse aspects of eating behaviour, we investigated whether OT acts there to affect appetite in rats. First, we examined whether direct AcbC and AcbSh OT injections affect hunger- and palatability-driven consumption. We found that only AcbC OT infusions decrease deprivation-induced chow intake and reduce the consumption of palatable sucrose and saccharin solutions in nondeprived animals. These effects were abolished by pretreatment with an OTR antagonist, L-368,899, injected in the same site. AcbC OT at an anorexigenic dose did not induce a conditioned taste aversion, which indicates that AcbC OT-driven anorexia is not caused by sickness/malaise. The appetite-specific effect of AcbC OT is supported by the real-time polymerase chain reaction analysis of OTR mRNA in the AcbC, which revealed that food deprivation elevates OTR mRNA expression, whereas saccharin solution intake decreases OTR transcript levels. We also used c-Fos immunohistochemistry as a marker of neuronal activation and found that AcbC OT injection increases activation of the AcbC itself, as well as of two feeding-related sites: the hypothalamic paraventricular and supraoptic nuclei. Finally, considering the fact that OT plays a significant role in social behaviour, we examined whether offering animals a meal in a social setting would modify their hypophagic response to AcbC OT injections. We found that a social context abolishes the anorexigenic effects of AcbC OT. We conclude that OT acting via the AcbC decreases food intake driven by hunger and reward in rats offered a meal in a nonsocial setting. © 2016

Distinct BOLD Activation Profiles Following Central and Peripheral Oxytocin Administration in Awake Rats.

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Ferris, Craig F; Yee, Jason R; Kenkel, William M; Dumais, Kelly Marie; Moore, Kelsey; Veenema, Alexa H; Kulkarni, Praveen; Perkybile, Allison M; Carter, C Sue

2015-01-01

A growing body of literature has suggested that intranasal oxytocin (OT) or other systemic routes of administration can alter prosocial behavior, presumably by directly activating OT sensitive neural circuits in the brain. Yet there is no clear evidence that OT given peripherally can cross the blood-brain barrier at levels sufficient to engage the OT receptor. To address this issue we examined changes in blood oxygen level-dependent (BOLD) signal intensity in response to peripheral OT injections (0.1, 0.5, or 2.5 mg/kg) during functional magnetic resonance imaging (fMRI) in awake rats imaged at 7.0 T. These data were compared to OT (1 μg/5 μl) given directly to the brain via the lateral cerebroventricle. Using a 3D annotated MRI atlas of the rat brain segmented into 171 brain areas and computational analysis, we reconstructed the distributed integrated neural circuits identified with BOLD fMRI following central and peripheral OT. Both routes of administration caused significant changes in BOLD signal within the first 10 min of administration. As expected, central OT activated a majority of brain areas known to express a high density of OT receptors, e.g., lateral septum, subiculum, shell of the accumbens, bed nucleus of the stria terminalis. This profile of activation was not matched by peripheral OT. The change in BOLD signal to peripheral OT did not show any discernible dose-response. Interestingly, peripheral OT affected all subdivisions of the olfactory bulb, in addition to the cerebellum and several brainstem areas relevant to the autonomic nervous system, including the solitary tract nucleus. The results from this imaging study do not support a direct central action of peripheral OT on the brain. Instead, the patterns of brain activity suggest that peripheral OT may interact at the level of the olfactory bulb and through sensory afferents from the autonomic nervous system to influence brain activity.

Transitional basal cells at the squamous-columnar junction generate Barrett’s oesophagus

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Jiang, Ming; Li, Haiyan; Zhang, Yongchun; Yang, Ying; Lu, Rong; Liu, Kuancan; Lin, Sijie; Lan, Xiaopeng; Wang, Haikun; Wu, Han; Zhu, Jian; Zhou, Zhongren; Xu, Jianming; Lee, Dong-Kee; Zhang, Lanjing; Lee, Yuan-Cho; Yuan, Jingsong; Abrams, Julian A.; Wang, Timothy G.; Sepulveda, Antonia R.; Wu, Qi; Chen, Huaiyong; Sun, Xin; She, Junjun; Chen, Xiaoxin; Que, Jianwen

2017-01-01

In several organ systems the transitional zone between different types of epithelia is a hotspot for pre-neoplastic metaplasia and malignancy1–3. However, the cell-of-origin for the metaplastic epithelium and subsequent malignancy, remains obscure1–3. In the case of Barrett’s oesophagus (BE), intestinal metaplasia occurs at the gastro-oesophageal junction, where stratified squamous epithelium transitions into simple columnar cells4. Based on different experimental models, several alternative cell types have been proposed as the source of the metaplasia, but in all cases the evidence is inconclusive and no model completely mimics BE with the presence of intestinal goblet cells5–8. Here, we describe a novel transitional columnar epithelium with distinct basal progenitor cells (p63+ KRT5+ KRT7+) in the squamous-columnar junction (SCJ) in the upper gastrointestinal tract of the mouse. We use multiple models and lineage tracing strategies to show that this unique SCJ basal cell population serves as a source of progenitors for the transitional epithelium. Moreover, upon ectopic expression of CDX2 these transitional basal progenitors differentiate into intestinal-like epithelium including goblet cells, thus reproducing Barrett’s metaplasia. A similar transitional columnar epithelium is present at the transitional zones of other mouse tissues, including the anorectal junction, and, importantly, at the gastro-oesophageal junction in the human gut. Acid reflux-induced oesophagitis and the multilayered epithelium (MLE) believed to be a precursor of BE are both characterized by the expansion of the transitional basal progenitor cells. Taken together our findings reveal the presence of a previously unidentified transitional zone in the epithelium of the upper gastrointestinal tract and provide evidence that the p63+ KRT7+ basal cells in this zone are the cell-of-origin for MLE and BE. PMID:29019984

Screening for the Most Suitable Reference Genes for Gene Expression Studies in Equine Milk Somatic Cells.

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Jakub Cieslak

Full Text Available Apart from the well-known role of somatic cell count as a parameter reflecting the inflammatory status of the mammary gland, the composition of cells isolated from milk is considered as a valuable material for gene expression studies in mammals. Due to its unique composition, in recent years an increasing interest in mare's milk consumption has been observed. Thus, investigating the genetic background of horse's milk variability presents and interesting study model. Relying on 39 milk samples collected from mares representing three breeds (Polish Primitive Horse, Polish Cold-blooded Horse, Polish Warmblood Horse we aimed to investigate the utility of equine milk somatic cells as a source of mRNA and to screen the best reference genes for RT-qPCR using geNorm and NormFinder algorithms. The results showed that despite relatively low somatic cell counts in mare's milk, the amount and the quality of the extracted RNA are sufficient for gene expression studies. The analysis of the utility of 7 potential reference genes for RT-qPCR experiments for the normalization of equine milk somatic cells revealed some differences between the outcomes of the applied algorithms, although in both cases the KRT8 and TOP2B genes were pointed as the most stable. Analysis by geNorm showed that the combination of 4 reference genes (ACTB, GAPDH, TOP2B and KRT8 is required for apropriate RT-qPCR experiments normalization, whereas NormFinder algorithm pointed the combination of KRT8 and RPS9 genes as the most suitable. The trial study of the relative transcript abundance of the beta-casein gene with the use of various types and numbers of internal control genes confirmed once again that the selection of proper reference gene combinations is crucial for the final results of each real-time PCR experiment.

Proteomic profiling reveals candidate markers for arsenic-induced skin keratosis.

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Guo, Zhiling; Hu, Qin; Tian, Jijing; Yan, Li; Jing, Chuanyong; Xie, Heidi Qunhui; Bao, Wenjun; Rice, Robert H; Zhao, Bin; Jiang, Guibin

2016-11-01

Proteomics technology is an attractive biomarker candidate discovery tool that can be applied to study large sets of biological molecules. To identify novel biomarkers and molecular targets in arsenic-induced skin lesions, we have determined the protein profile of arsenic-affected human epidermal stratum corneum by shotgun proteomics. Samples of palm and foot sole from healthy subjects were analyzed, demonstrating similar protein patterns in palm and sole. Samples were collected from the palms of subjects with arsenic keratosis (lesional and adjacent non-lesional samples) and arsenic-exposed subjects without lesions (normal). Samples from non-exposed healthy individuals served as controls. We found that three proteins in arsenic-exposed lesional epidermis were consistently distinguishably expressed from the unaffected epidermis. One of these proteins, the cadherin-like transmembrane glycoprotein, desmoglein 1 (DSG1) was suppressed. Down-regulation of DSG1 may lead to reduced cell-cell adhesion, resulting in abnormal epidermal differentiation. The expression of keratin 6c (KRT6C) and fatty acid binding protein 5 (FABP5) were significantly increased. FABP5 is an intracellular lipid chaperone that plays an essential role in fatty acid metabolism in human skin. This raises a possibility that overexpression of FABP5 may affect the proliferation or differentiation of keratinocytes by altering lipid metabolism. KRT6C is a constituent of the cytoskeleton that maintains epidermal integrity and cohesion. Abnormal expression of KRT6C may affect its structural role in the epidermis. Our findings suggest an important approach for future studies of arsenic-mediated toxicity and skin cancer, where certain proteins may represent useful biomarkers of early diagnoses in high-risk populations and hopefully new treatment targets. Further studies are required to understand the biological role of these markers in skin pathogenesis from arsenic exposure. Copyright © 2016 Elsevier Ltd

Developmental toxicity of organotin compounds in animals

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Lijiao eWu

2014-09-01

Full Text Available Organotin compounds (OTs have been used as biocides in antifouling paints and agriculture. The IMO introduced a global ban on the use of OTs in antifouling systems in 2001 due to their high toxicity. However, OTs have still been detected in the environment and pose a threat to the ecosystem. Several research groups have summarized the analytical methods, environmental fate, biochemistry, reproductive toxicity and mechanisms of actions of OTs. Here, we reviewed the developmental toxicity of OTs in various organisms such as sea urchin, ascidian, mussel and fish. The differences in sensitivity to OT exposure exist not only in different species but also at different stages in the same species. Though some hypotheses have been proposed to explain the developmental toxicity of OTs, the solid evidences are greatly in need.

A Comparison of Gains in Keypad Response Technology Classroom with Other Pedagogies in Nigeria

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Agbatogun, Alaba Olaoluwakotansibe

2013-01-01

Drawing from the socio-cultural theory of Vygotsky, this study compared the academic performance gains scores of pupils in a Keypad Response Technology (KRT) class with those in two other pedagogies to determine the learners' language proficiency level in ESL classroom. A sample of 99 Nigerian primary six pupils participated in the study.…

Plasma oxytocin concentrations are lower in depressed vs. healthy control women and are independent of cortisol.

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Yuen, Kaeli W; Garner, Joseph P; Carson, Dean S; Keller, Jennifer; Lembke, Anna; Hyde, Shellie A; Kenna, Heather A; Tennakoon, Lakshika; Schatzberg, Alan F; Parker, Karen J

2014-04-01

The neuropeptide oxytocin (OT) promotes social behavior and attenuates stress responsivity in mammals. Recent clinical evidence suggests OT concentrations may be dysregulated in major depression. This study extends previous research by testing whether: 1) OT concentrations vary systematically in depressive disorders with and without hypercortisolemia, 2) gender differences in OT concentrations are observed in depressed vs. healthy control participants, and 3) OT concentrations are predictive of clinical phenotypes. Plasma OT concentrations of psychotic major depressive (PMD; n = 14: 10 female, 4 male), non-psychotic major depressive (NPMD; n = 17: 12 female, 5 male), and non-depressed, healthy control (n = 19: 11 female, 8 male) participants were assayed at 2000, 2400, 0400, and 0800 h. Plasma cortisol concentrations were quantified at 2300 h, and clinical phenotypes were determined. As expected, PMD participants, compared to NPMD and healthy control participants, showed higher plasma cortisol concentrations. Although both depressed groups showed similar OT concentrations, a significant interaction effect between group and gender was observed. Specifically, depressed females exhibited lower mean OT concentrations than depressed males. Further, depressed vs. healthy control female participants exhibited lower mean OT concentrations, whereas depressed vs. healthy control male participants showed a trend in the opposite direction. OT concentrations were also predictive of desirability, drug dependence, and compulsivity scores as measured by the Million Clinical Multiaxial Inventory-III. All findings were independent of cortisol. These data suggest that OT signaling may provide a mechanism by which to better understand female-biased risk to develop depressive disorders and that plasma OT concentrations may be a useful biomarker of certain clinical phenotypes. Copyright © 2013. Published by Elsevier Ltd.

Oxytocin mechanisms of stress response and aggression in a territorial finch.

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Goodson, James L; Schrock, Sara E; Kingsbury, Marcy A

2015-03-15

All jawed vertebrates produce a form of oxytocin (OT), and in birds, mammals and fish, OT is strongly associated with affiliation. However, remarkably few data are available on the roles of OT and OT receptors (OTRs) in aggression. Because OT and OTRs exert anxiolytic effects in mammals (although context-specific) and modulate stress coping, we hypothesized that OTR activation is at least permissive for territorial aggression. Indeed, we find that peripheral injections of an OTR antagonist significantly reduce male-male and female-female aggression in a highly territorial finch. This finding suggests the hypothesis that aggression is accompanied by an increase in transcriptional (Fos) activity of OT neurons, but contrary to this hypothesis, we find that dominant male residents do not elevate OT-Fos colocalization following an aggressive encounter and that OT-Fos colocalization in the preoptic area and hypothalamus correlates negatively with aggression. Furthermore, OT-Fos colocalization increases dramatically in males that were aggressively subjugated or pursued by a human hand, likely reflecting OT modulation of stress response. Because OT inhibits the hypothalamo-pituitary-adrenal axis, the antagonist effects may reflect the fact that aggressive birds and mammals tend to be hyporesponsive to stress. If this is correct, then 1) the observed effects of OTR antagonism may reflect alterations in corticosterone feedback to the brain rather than centrally mediated OTR effects, and 2) the negative correlation between OT-Fos colocalization and aggression may reflect the fact that more aggressive, stress hyporesponsive males require less inhibition of the hypothalamo-pituitary-adrenal axis than do less aggressive males, despite the requirement of that inhibition for the normal display of aggression. Copyright © 2015 Elsevier Inc. All rights reserved.

Betoonipidu Kumus

Index Scriptorium Estoniae

2006-01-01

15. III betoonipäeval anti üle konkursi "Aasta betoonehitis 2005" auhinnad. Aasta betoonehitis - TTP kontorihoone Pirital (peapreemia - arhitekt Meelis Press, ehitaja auhind - OÜ Roxor Ehitus, betoonitarnija - AS HC Betoon); arhitektuuri eriauhinnad - QP Arhitektid (arhihektid Tõnu Laigu, Tõnu Laanemäe), AS-i Coal Terminal peahoone, 3+1 Arhitektid, eramu Magasini kvartalis Tallinnas; ehitaja eriauhind - AS Merko Ehitus, Kumu hoone; Eesti Päevalehe lugejate lemmik - AS TTP kontorihoone; ajakirja Ehitaja eripreemia - arhitekt Marti Aunin, büroohoone Tetris Tallinnas

Tööpuudus hiilib kikivarvul Võrumaale / Arved Breidaks

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Breidaks, Arved, 1975-

2008-01-01

Võrumaal on töötute arv eelmise aastaga võrreldes tõusma hakanud: mullu suvel maakonnas registreeritud 3,9%-line töötuse määr asendus tänavu juuli lõpus 5,4%-lise töötusega. Lisa: Töötus Võrumaal. Vt. samas: Kuidas iseloomustate olukorda Võrumaa tööjõuturul? Vastavad Martin Arula (AS Toftan), Kaido Mäesalu (AS Suwem), Meelis Munski (AS Semuehitus), Indrek Klampe (OÜ Selista Ehitus), Andres Visanpuu (Võru TÜ)

Oxytocin increases amygdala reactivity to threatening scenes in females.

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Lischke, Alexander; Gamer, Matthias; Berger, Christoph; Grossmann, Annette; Hauenstein, Karlheinz; Heinrichs, Markus; Herpertz, Sabine C; Domes, Gregor

2012-09-01

The neuropeptide oxytocin (OT) is well known for its profound effects on social behavior, which appear to be mediated by an OT-dependent modulation of amygdala activity in the context of social stimuli. In humans, OT decreases amygdala reactivity to threatening faces in males, but enhances amygdala reactivity to similar faces in females, suggesting sex-specific differences in OT-dependent threat-processing. To further explore whether OT generally enhances amygdala-dependent threat-processing in females, we used functional magnetic resonance imaging (fMRI) in a randomized within-subject crossover design to measure amygdala activity in response to threatening and non-threatening scenes in 14 females following intranasal administration of OT or placebo. Participants' eye movements were recorded to investigate whether an OT-dependent modulation of amygdala activity is accompanied by enhanced exploration of salient scene features. Although OT had no effect on participants' gazing behavior, it increased amygdala reactivity to scenes depicting social and non-social threat. In females, OT may, thus, enhance the detection of threatening stimuli in the environment, potentially by interacting with gonadal steroids, such as progesterone and estrogen. Copyright © 2012 Elsevier Ltd. All rights reserved.

Oxytocin and Aggression.

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de Jong, Trynke R; Neumann, Inga D

2017-09-02

The neuropeptide oxytocin (OT) has a solid reputation as a facilitator of social interactions such as parental and pair bonding, trust, and empathy. The many results supporting a pro-social role of OT have generated the hypothesis that impairments in the endogenous OT system may lead to antisocial behavior, most notably social withdrawal or pathological aggression. If this is indeed the case, administration of exogenous OT could be the "serenic" treatment that psychiatrists have for decades been searching for.In the present review, we list and discuss the evidence for an endogenous "hypo-oxytocinergic state" underlying aggressive and antisocial behavior, derived from both animal and human studies. We furthermore examine the reported effects of synthetic OT administration on aggression in rodents and humans.Although the scientific findings listed in this review support, in broad lines, the link between a down-regulated or impaired OT system activity and increased aggression, the anti-aggressive effects of synthetic OT are less straightforward and require further research. The rather complex picture that emerges adds to the ongoing debate questioning the unidirectional pro-social role of OT, as well as the strength of the effects of intranasal OT administration in humans.

The knowledge and attitudes of orthodontic trainees towards orthodontic therapists: a national survey.

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Patel, Sameer; Mack, Gavin

2017-09-01

To assess the knowledge and attitudes of orthodontic trainees towards orthodontic therapists (OTs) in the UK. Cross-sectional survey. UK-based orthodontic trainees. An electronic survey was sent to all members of the Training Grades Group of the British Orthodontic Society assessing exposure to OTs and their knowledge regarding current supervision guidelines and scope of practice. Attitudes towards OTs were also explored. Seventy-six responses (response rate 57%) were returned. Nearly 90% of trainees had no formal training regarding OTs. A total of 15.5% were aware of the correct current supervision guidelines and there was large variation in the knowledge of OTs' scope of practice. The majority of trainees were happy to supervise OTs, but only 22.4% felt prepared for this during training. In total, 63% of trainees felt that OTs could impact their own future job prospects. Currently, there is minimal formal training provided to trainees regarding the role of OTs. This is reflected in the lack of knowledge regarding supervision guidelines and scope of practice. Overall, trainees felt OTs were positive for the workforce but were concerned regarding the impact of their own future employment.

Matrjoshki ot kutjur

Index Scriptorium Estoniae

2008-01-01

Vene moeajakirja Vogue kümnendaks juubeliks joonistasid tuntud moekunstnikud kogu maailmast matrjoshkade eskiise. Eskiiside järgi maalisid vene meistrid poolemeetrised nukud. Nii sai ajakiri kingituseks Yves Saint Laurent'i, Versace, Prada, Dolce&Gabbana, Oscar de la Renta, Judashkini, Simatshovi ja paljude teiste matrjoshkad

Dissociating frontal regions that co-lateralize with different ventral occipitotemporal regions during word processing☆

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Seghier, Mohamed L.; Price, Cathy J.

2013-01-01

The ventral occipitotemporal sulcus (vOT) sustains strong interactions with the inferior frontal cortex during word processing. Consequently, activation in both regions co-lateralize towards the same hemisphere in healthy subjects. Because the determinants of lateralisation differ across posterior, middle and anterior vOT subregions, we investigated whether lateralisation in different inferior frontal regions would co-vary with lateralisation in the three different vOT subregions. A whole brain analysis found that, during semantic decisions on written words, laterality covaried in (1) posterior vOT and the precentral gyrus; (2) middle vOT and the pars opercularis, pars triangularis, and supramarginal gyrus; and (3) anterior vOT and the pars orbitalis, middle frontal gyrus and thalamus. These findings increase the spatial resolution of our understanding of how vOT interacts with other brain areas during semantic categorisation on words. PMID:23728081

Neuropeptide diversity and the regulation of social behavior in New World primates

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French, Jeffrey A.; Taylor, Jack H.; Mustoe, Aaryn C.; Cavanaugh, Jon

2016-01-01

Oxytocin (OT) and vasopressin (AVP) are important hypothalamic neuropeptides that regulate peripheral physiology, and have emerged as important modulators of brain function, particularly in the social realm. OT structure and the genes that ultimately determine structure are highly conserved among diverse eutherian mammals, but recent discoveries have identified surprising variability in OT and peptide structure in New World monkeys (NWM), with five new OT variants identified to date. This review explores these new findings in light of comparative OT/AVP ligand evolution, documents coevolutionary changes in the oxytocin and vasopressin receptors (OTR and V1aR), and highlights the distribution of neuropeptidergic neurons and receptors in the primate brain. Finally, the behavioral consequences of OT and AVP in regulating NWM sociality are summarized, demonstrating important neuromodulatory effects of these compounds and OT ligand-specific influences in certain social domains. PMID:27020799

Oxytocin--its role in male reproduction and new potential therapeutic uses.

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Thackare, Hemlata; Nicholson, Helen D; Whittington, Kate

2006-01-01

Oxytocin (OT) is traditionally thought of as a "female" neurohypophysis hormone due to its role in parturition and milk ejection. However, OT is recognized as having endocrine and paracrine roles in male reproduction. At ejaculation, a burst of OT is released from the neurohypophysis into the systemic circulation and stimulates contractions of the reproductive tract aiding sperm release. There is conclusive evidence that OT is synthesized within the mammalian testis, epididymis and prostate and the presence of OT receptors (OTRs) through the reproductive tract supports a local action for this peptide. OT has a paracrine role in stimulating contractility of the seminiferous tubules, epididymis and the prostate gland. Interestingly, OT has also been shown to modulate androgen levels in these tissues via stimulation of the conversion of testosterone to dihydrotestostone (DHT) by 5alpha-reductase. The elucidation of OT's role in male reproduction has suggested a number of potential therapeutic uses for this hormone. Exogenous administration of OT has, in some cases, been shown to increase the numbers of ejaculated sperm, possibly by stimulating contractions of the reproductive tract and thus aiding sperm passage. Within the prostate, OT has been shown to affect gland growth both directly and via its interaction with androgen metabolism. Prostate pathologies due to unregulated cell proliferation/growth, such as benign prostatic hyperplasia and cancer, are unfortunately very common and few effective treatments are available. Greater understanding of paracrine growth mediators, such as OT, is likely to provide new mechanisms for treating such pathologies.

An Early Postnatal Oxytocin Treatment Prevents Social and Learning Deficits in Adult Mice Deficient for Magel2, a Gene Involved in Prader-Willi Syndrome and Autism.

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Meziane, Hamid; Schaller, Fabienne; Bauer, Sylvian; Villard, Claude; Matarazzo, Valery; Riet, Fabrice; Guillon, Gilles; Lafitte, Daniel; Desarmenien, Michel G; Tauber, Maithé; Muscatelli, Françoise

2015-07-15

Mutations of MAGEL2 have been reported in patients presenting with autism, and loss of MAGEL2 is also associated with Prader-Willi syndrome, a neurodevelopmental genetic disorder. This study aimed to determine the behavioral phenotype of Magel2-deficient adult mice, to characterize the central oxytocin (OT) system of these mutant mice, and to test the curative effect of a peripheral OT treatment just after birth. We assessed the social and cognitive behavior of Magel2-deficient mice, analyzed the OT system of mutant mice treated or not by a postnatal administration of OT, and determined the effect of this treatment on the brain. Magel2 inactivation induces a deficit in social recognition and social interaction and a reduced learning ability in adult male mice. In these mice, we reveal anatomical and functional modifications of the OT system and show that these defects change from birth to adulthood. Daily administration of OT in the first postnatal week was sufficient to prevent deficits in social behavior and learning abilities in adult mutant male mice. We show that this OT treatment partly restores a normal OT system. Thus, we report that an alteration of the OT system around birth has long-term consequences on behavior and on cognition. Importantly, an acute OT treatment of Magel2-deficient pups has a curative effect. Our study reveals that OT plays a crucial role in setting social behaviors during a period just after birth. An early OT treatment in this critical period could be a novel therapeutic approach for the treatment of neurodevelopmental disorders such as Prader-Willi syndrome and autism. Copyright © 2015 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved.

Concentration of Antifouling Biocides and Metals in Sediment Core Samples in the Northern Part of Hiroshima Bay

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Noritaka Tsunemasa

2014-06-01

Full Text Available Accumulation of Ot alternative antifoulants in sediment is the focus of this research. Much research had been done on surface sediment, but in this report, the accumulation in the sediment core was studied. The Ot alternative antifoulants, Diuron, Sea-Nine211, and Irgarol 1051, and the latter’s degradation product, M1, were investigated in five samples from the northern part of Hiroshima Bay. Ot compounds (tributyltin (TBT and triphenyltin (TPT were also investigated for comparison. In addition, metal (Pb, Cu, Zn, Fe and Mn levels and chronology were measured to better understand what happens after accumulation on the sea floor. It was discovered that Ot alternative antifoulant accumulation characteristics in sediment were like Ot compounds, with the concentration in the sediment core being much higher than surface sediment. The concentration in sediment seems to have been affected by the regulation of Ot compounds in 1990, due to the concentration of Ot alternative antifoulants and Ot compounds at the survey point in front of the dock, showing an increase from almost the same layer after the regulation.

[Interdisciplinary teamwork in the OR: Identification and measurement of teamwork in the operating room].

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Passauer-Baierl, Stefanie; Baschnegger, Heiko; Bruns, Christiane; Weigl, Matthias

2014-01-01

Effective teamwork is one of the essentials in conducting successful and safe surgical procedures in the operating theatre (OT). The present paper aims to provide a selective review of various approaches describing effective interdisciplinary teamwork in the OT. Furthermore, it covers observational methods to assess OT teamwork with particular focus on Germany. Our definition of successful surgical teamwork is based on an already established classification system considering five criteria for effective and safe OT teams: coordination, communication, cooperation, leadership, and team monitoring. Well-defined and reliable measures are necessary to examine the quality of OT teamwork. Those methods should entail the special characteristics of the OT team. They should include all phases of the surgical procedure and incorporate all the professions involved (surgeons, surgical nurses, and anaesthetic staff). We conclude that research into methods for the assessment of OTs in Germany needs to be undertaken as a prerequisite to investigating the relationship between OT teamwork and its effects on patient safety and surgical quality. Copyright © 2013. Published by Elsevier GmbH.

Sex, Receptors and Attachment: A Review of Individual Factors Influencing Response to Oxytocin

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Kai S Macdonald

2013-01-01

Full Text Available As discussed in the larger review in this special issue (MacDonald and Feifel, intranasal oxytocin (IN OT is demonstrating a growing potential as a therapeutic agent in psychiatry. Importantly, research suggests that a variety of individual factors may influence a person’s response to OT. In this mini-review, I provides a review of three: (1 sex and hormonal status; (2 genetic variation in aspects of the OT system (i.e. OT receptors; and (3 attachment history. Each of these factors will be important to monitor as we strive to develop a richer understanding of OT's role in human development, brain-based disease, and the potential for individualized, OT-targeted treatments.

A determination of occlusal plane comparing different levels of the tragus to form ala-tragal line or Camper's line: A photographic study.

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Kumar, Sandeep; Garg, Sandeep; Gupta, Seema

2013-02-01

The purpose of this study was to determine accurately the part of the tragus to be used to form the Ala-Tragal line or Camper's line in orthognathic profile patients. 150 dentate subjects with age of 18-40 years with orthognathic profile were sampled. Life-size lateral digital photographs of the face with fox plane were taken in natural head position. Different angles between Eye-Ear plane and occlusal plane (OT1-OP), Eye-Ear plane and ala-superior border of tragus (OT1-AT1), Eye-Ear plane and ala-middle border of tragus (OT1-AT2) and Eye-Ear plane and ala-inferior border of tragus (OT1-AT3) were calculated using computer software package, AutoCAD 2004. From the three angles formed by the Eye-ear plane (OT1 or FH plane) and the ala-tragal lines, the one closest to the angle formed between Eye-Ear plane (OT1) and occlusal plane (OP) was used to determine the occlusal plane of orientation. The obtained results were subjected to ANOVA F test, Tukey's Honestly significant difference test, followed by Karl Pearson coefficient of correlation test. P values of less than 0.05 were taken as statistically significant. The mean of base line angle i.e. OT1-OP angle (11.96 ± 4.36) was found to be close to OT1-AT2 angle (13.67 ± 1.93) and OT1-AT3 angle (10.31 ± 2.03), but OT1-OP angle was found to be more closer to OT1-AT3 angle. Comparison of mean angles showed that OT1-OP angle in both males (11.68) and females (12.51) is close to OT1-AT3 angle (males- 11.01, females- 11.95). The line joining from ala to the lower border of the tragus was parallel to the occlusal plane in 53.3% of the subjects. There was no influence of the sex on the level of occlusal plane.

Organotin Exposure and Vertebrate Reproduction: A Review

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Julia Fernandez Puñal de Araújo

2018-03-01

Full Text Available Organotin (OTs compounds are organometallic compounds that are widely used in industry, such as in the manufacture of plastics, pesticides, paints, and others. OTs are released into the environment by anthropogenic actions, leading to contact with aquatic and terrestrial organisms that occur in animal feeding. Although OTs are degraded environmentally, reports have shown the effects of this contamination over the years because it can affect organisms of different trophic levels. OTs act as endocrine-disrupting chemicals (EDCs, which can lead to several abnormalities in organisms. In male animals, OTs decrease the weights of the testis and epididymis and reduce the spermatid count, among other dysfunctions. In female animals, OTs alter the weights of the ovaries and uteri and induce damage to the ovaries. In addition, OTs prevent fetal implantation and reduce mammalian pregnancy rates. OTs cross the placental barrier and accumulate in the placental and fetal tissues. Exposure to OTs in utero leads to the accumulation of lipid droplets in the Sertoli cells and gonocytes of male offspring in addition to inducing early puberty in females. In both genders, this damage is associated with the imbalance of sex hormones and the modulation of the hypothalamic–pituitary–gonadal axis. Here, we report that OTs act as reproductive disruptors in vertebrate studies; among the compounds are tetrabutyltin, tributyltin chloride, tributyltin acetate, triphenyltin chloride, triphenyltin hydroxide, dibutyltin chloride, dibutyltin dichloride, diphenyltin dichloride, monobutyltin, and azocyclotin.

Antinociceptive action of oxytocin involves inhibition of potassium channel currents in lamina II neurons of the rat spinal cord

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Darbon Pascal

2009-11-01

Full Text Available Abstract Background Growing evidence in the literature shows that oxytocin (OT has a strong spinal anti-nociceptive action. Oxytocinergic axons originating from a subpopulation of paraventricular hypothalamic neurons establish synaptic contacts with lamina II interneurons but little is known about the functional role of OT with respect to neuronal firing and excitability. Results Using the patch-clamp technique, we have recorded lamina II interneurons in acute transverse lumbar spinal cord slices of rats (15 to 30 days old and analyzed the OT effects on action potential firing ability. In the current clamp mode, we found that bath application of a selective OT-receptor agonist (TGOT reduced firing in the majority of lamina II interneurons exhibiting a bursting firing profile, but never in those exhibiting a single spike discharge upon depolarization. Interestingly, OT-induced reduction in spike frequency and increase of firing threshold were often observed, leading to a conversion of the firing profile from repetitive and delayed profiles into phasic ones and sometimes further into single spike profile. The observed effects following OT-receptor activation were completely abolished when the OT-receptor agonist was co-applied with a selective OT-receptor antagonist. In current and voltage clamp modes, we show that these changes in firing are strongly controlled by voltage-gated potassium currents. More precisely, transient IA currents and delayed-rectifier currents were reduced in amplitude and transient IA current was predominantly inactivated after OT bath application. Conclusion This effect of OT on the firing profile of lamina II neurons is in good agreement with the antinociceptive and analgesic properties of OT described in vivo.

Conditioned social preference, but not place preference, produced by intranasal oxytocin in female mice.

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Kosaki, Yutaka; Watanabe, Shigeru

2016-04-01

Oxytocin (OT) has been implicated in a variety of mammalian reproductive and social behaviors, and the use of intranasal OT for clinical purposes is on the rise. However, basic actions of OT, including the rewarding or reinforcing properties of the drug, are currently not fully understood. In this study, the authors investigated whether intranasally administered OT has different reinforcing properties for social and nonsocial stimuli and whether such effects are variable between male and female subjects. Conditioned social preference (CSP) and conditioned place preference (CPP) paradigms were used to examine social and nonsocial reinforcing properties of OT. In CSP, the presence of a same-sex unfamiliar conspecific was repeatedly paired with intranasal OT, while a different conspecific was associated with saline. The reinforcing effect of OT was assessed in a postconditioning choice test under a drug-free condition. In CPP, the 2 conspecifics were replaced with nonsocial black and white compartments. The authors found that intranasal OT (12 μg) in females supported the formation of CSP (Experiment 1) but not CPP (Experiment 3). Neither CSP (Experiment 2) nor CPP (Experiment 4) was formed in males. Extended conditioning with higher dose OT (36 μg), however, abolished the initial CSP in females and produced an aversion to the OT-paired stimulus mouse. Experiment 5 indicated that it was the repeated administrations rather than the higher dose that produced the abolition of the original preference. Overall, the current results demonstrate for the first time a sex- and stimulus-dependent reinforcing property of intranasal OT in mice. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

Kolleegist parem / Andres Kurg

Index Scriptorium Estoniae

Kurg, Andres, 1975-

2006-01-01

Eesti Sisearhitektide Liidu aastapreemiad 2005: parima ühiskondliku interjööri preemia võitis Pääsküla raamatukogu (Katrin Kaevats, Urmas Muru, Peeter Pere), näitusekujunduse preemia sai Maanteemuuseum (Krista Lepland, Malle Jürgenson, Tea Tammelaan, OÜ Laika, Belka & Strelka), ajaloolise interjööri preemia läks renoveeritud Estonia teatrile (Juta Lember, Aulo Padar), publitsistikapreemia sai Leila Pärtelpoja raamat "Pööningul", teise ühiskondliku ruumi preemia võitis TTP ärihoone Pirital (Meelis Press). Kommentaar Tiina Jõgedalt

Arnold Rüütel vassis taas aastaarvuga / Anneli Ammas

Index Scriptorium Estoniae

Ammas, Anneli, 1962-

2006-01-01

President Arnold Rüütel väitis 31. augusti Eesti Päevalehes, et temast sai kommunistlik noor 1949. aastal, autori sõnul algas A. Rüütli liikmelisus ELKNÜ-s juba 1946. aastal. Lähiajaloo uurija Meelis Maripuu väitel ei olnud 1946. aastal ELKNÜ veel massiorganisatsioon. Vt. samas: Varasem intervjuu - see oli 1949, pärast küüditamist; Rüütel: ma pole midagi salanud ; Ilves: Eesti vajab iga inimest. Toomas Hendrik Ilvese avaldusest seoses Johannes Hindi kohtuasja käsitlemisega ajakirjanduses

Jõulujazz alustab heaga / Kai Ilustrumm

Index Scriptorium Estoniae

Ilustrumm, Kai

2004-01-01

Heategevuskontserdist "Aita alustada elu!" Kaarli kirikus 28. nov., Soome multiinstrumentalisti Sakari Kukko trio kontserdist 5. dets. Niguliste muuseum-kontserdisaalis, ameerika vokaalansambli M-Pact kontserdist 7. dets. Pärnu kontserdimajas, Raivo Tafenau-Meelis Vindi koosmusitseerimisest tšiili löökpillimängija Ricardo Padillaga 11. dets. Niguliste muuseum-kontserisaalis, Helin-Mari Arderi kontserdist "Sajab esimest lund" 15. dets. Niguliste muuseum-kontserisaalis, rootsi ansambli Bossa Electrica esinemiset 16. dets. Rock Café's, ansambli Eesti Keeled kontserdist "Vaiksel ööl" 17. dets. Tallinnas Sokos Hotell Virus

Non-orthogonally transitive G2 spike solution

International Nuclear Information System (INIS)

Lim, Woei Chet

2015-01-01

We generalize the orthogonally transitive (OT) G 2 spike solution to the non-OT G 2 case. This is achieved by applying Geroch’s transformation on a Kasner seed. The new solution contains two more parameters than the OT G 2 spike solution. Unlike the OT G 2 spike solution, the new solution always resolves its spike. (fast track communication)

Investigations of the 0.020-scale 88-OTS Integrated Space Shuttle Vehicle Jet-Plume Model in the NASA/Ames Research Center 11 by11-Foot Unitary Plan Wind Tunnel (IA80). Volume 1

Science.gov (United States)

Nichols, M. E.

1976-01-01

The results are documented of jet plume effects wind tunnel test of the 0.020-scale 88-OTS launch configuration space shuttle vehicle model in the 11 x 11 foot leg of the NASA/Ames Research Center Unitary Plan Wind Tunnel. This test involved cold gas main propulsion system (MPS) and solid rocket motor (SRB) plume simulations at Mach numbers from 0.6 to 1.4. Integrated vehicle surface pressure distributions, elevon and rudder hinge moments, and wing and vertical tail root bending and torsional moments due to MPS and SRB plume interactions were determined. Nozzle power conditions were controlled per pretest nozzle calibrations. Model angle of attack was varied from -4 deg to +4 deg; model angle of sideslip was varied from -4 deg to +4 deg. Reynolds number was varied for certain test conditions and configurations, with the nominal freestream total pressure being 14.69 psia. Plotted force and pressure data are presented.

AFM investigation of effect of absorbed water layer structure on growth mechanism of octadecyltrichlorosilane self-assembled monolayer on oxidized silicon

International Nuclear Information System (INIS)

Li, Shaowei; Zheng, Yanjun; Chen, Changfeng

2016-01-01

The growth mechanism of an octadecyltrichlorosilane (OTS) self-assembled monolayer on a silicon oxide surface at various relative humidities has been investigated. Atomic force microscopy images show that excess water may actually hinder the nucleation and growth of OTS islands. A moderate amount of water is favorable for the nucleation and growth of OTS islands in the initial stage; however, the completion of the monolayer is very slow in the final stage. The growth of OTS islands on a low-water-content surface maintains a relatively constant speed and requires the least amount of time. The mobility of water molecules is thought to play an important role in the OTS monolayers, and a low-mobility water layer provides a steady condition for OTS monolayer growth.

Role of oxytocin in improving the welfare of farm animals - A review.

Science.gov (United States)

Chen, Siyu; Sato, Shusuke

2017-04-01

Recently, increasing attention has been paid to the welfare of farm animals, which have been evaluated using behavioral and physiological measures. However, so far, the measures have almost always been used to estimate poor welfare. In this review, firstly we focus on how oxytocin (OT) relates to positive social behavior, pleasure, and stress tolerance, and second on which management factors stimulate OT release. OT induces maternal and affiliative behaviors and has an anti-stress effect. Further, OT is produced during enjoyable events, and has positive feedback on its own release as well. Therefore, to some extent, the relationship of OT to positive normal behavior was mutually beneficial-heightened OT concentration owing to comfortable rearing conditions induces positive social behavior, which in turn may increase OT concentration. Hence, studies on animal welfare should pay more attention to increasing comfort and the stress tolerance, rather than only focusing on when stress occurs in farm animals.

Vasopressin and Oxytocin Reduce Food Sharing Behavior in Male, but Not Female Marmosets in Family Groups

Directory of Open Access Journals (Sweden)

Jack H. Taylor

2017-07-01

Full Text Available Oxytocin (OT is critical for lactation and maternal care, but OT and the related nonapeptide vasopressin are important for caregiving behaviors in fathers and alloparents as well. This experiment tested the effects of vasopressin and OT on food sharing in marmoset families. We treated caregivers (parents, siblings with intranasal vasopressin, OT, or saline, and then paired them with the youngest marmoset in the family. Caregivers were given preferred food, and then observed for food sharing and aggressive behavior with young marmosets. OT reduced food sharing from male alloparents to youngest siblings, and fathers that received vasopressin refused to share food with their youngest offspring more often than when treated with OT. Vasopressin increased aggressive vocalizations directed toward potential food recipients in all classes of caregivers. These results indicate that vasopressin and OT do not always enhance prosocial behavior: modulation of food sharing depends on both sex and parental status.

Orthoptic Treatment of Convergence Insufficiency in Parkinson's Disease: A Case Series.

Science.gov (United States)

Kergoat, Hélène; Law, Caroline; Chriqui, Estefania; Kergoat, Marie-Jeanne; Leclerc, Bernard-Simon; Panisset, Michel; Postuma, Ronald; Irving, Elizabeth L

2017-01-01

Introduction: This study reports a case series of orthoptic treatment (OT) for convergence insufficiency (CI) in individuals with Parkinson's disease (PD). Method: We are reporting two cases of individuals with PD who completed OT for CI. Both had a confirmed diagnosis of CI, accompanied by CI-type symptomatology. They each underwent an OT program consisting of three office-based visits and 8 weeks of home-based exercises. Treatment outcome was based on the changes measured pre- versus post-OT on the near point of convergence, positive fusional vergences, and symptomatology score. Results: The two participants successfully completed therapy, gained ability to converge, had fewer symptoms, and were satisfied with the OT-induced changes they felt in their day-to-day lives. Conclusion: This case series show that OT for CI in PD is possible. Further research is required as these results demonstrate that OT has the potential to improve symptomatic CI in these patients. In the meantime, the positive results obtained in these two cases should encourage clinicians to consider OT (a therapy with no/minimal risk) for CI in patients with PD whose quality of life is affected by this binocular dysfunction.

Influence of oxytocin on emotion recognition from body language: A randomized placebo-controlled trial.

Science.gov (United States)

Bernaerts, Sylvie; Berra, Emmely; Wenderoth, Nicole; Alaerts, Kaat

2016-10-01

The neuropeptide 'oxytocin' (OT) is known to play a pivotal role in a variety of complex social behaviors by promoting a prosocial attitude and interpersonal bonding. One mechanism by which OT is hypothesized to promote prosocial behavior is by enhancing the processing of socially relevant information from the environment. With the present study, we explored to what extent OT can alter the 'reading' of emotional body language as presented by impoverished biological motion point light displays (PLDs). To do so, a double-blind between-subjects randomized placebo-controlled trial was conducted, assessing performance on a bodily emotion recognition task in healthy adult males before and after a single-dose of intranasal OT (24 IU). Overall, a single-dose of OT administration had a significant effect of medium size on emotion recognition from body language. OT-induced improvements in emotion recognition were not differentially modulated by the emotional valence of the presented stimuli (positive versus negative) and also, the overall tendency to label an observed emotional state as 'happy' (positive) or 'angry' (negative) was not modified by the administration of OT. Albeit moderate, the present findings of OT-induced improvements in bodily emotion recognition from whole-body PLD provide further support for a link between OT and the processing of socio-communicative cues originating from the body of others. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Statistical and Methodological Considerations for the Interpretation of Intranasal Oxytocin Studies.

Science.gov (United States)

Walum, Hasse; Waldman, Irwin D; Young, Larry J

2016-02-01

Over the last decade, oxytocin (OT) has received focus in numerous studies associating intranasal administration of this peptide with various aspects of human social behavior. These studies in humans are inspired by animal research, especially in rodents, showing that central manipulations of the OT system affect behavioral phenotypes related to social cognition, including parental behavior, social bonding, and individual recognition. Taken together, these studies in humans appear to provide compelling, but sometimes bewildering, evidence for the role of OT in influencing a vast array of complex social cognitive processes in humans. In this article, we investigate to what extent the human intranasal OT literature lends support to the hypothesis that intranasal OT consistently influences a wide spectrum of social behavior in humans. We do this by considering statistical features of studies within this field, including factors like statistical power, prestudy odds, and bias. Our conclusion is that intranasal OT studies are generally underpowered and that there is a high probability that most of the published intranasal OT findings do not represent true effects. Thus, the remarkable reports that intranasal OT influences a large number of human social behaviors should be viewed with healthy skepticism, and we make recommendations to improve the reliability of human OT studies in the future. Copyright © 2016 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved.

A common oxytocin receptor gene (OXTR) polymorphism modulates intranasal oxytocin effects on the neural response to social cooperation in humans.

Science.gov (United States)

Feng, C; Lori, A; Waldman, I D; Binder, E B; Haroon, E; Rilling, J K

2015-09-01

Intranasal oxytocin (OT) can modulate social-emotional functioning and related brain activity in humans. Consequently, OT has been discussed as a potential treatment for psychiatric disorders involving social behavioral deficits. However, OT effects are often heterogeneous across individuals. Here we explore individual differences in OT effects on the neural response to social cooperation as a function of the rs53576 polymorphism of the oxytocin receptor gene (OXTR). Previously, we conducted a double-blind, placebo-controlled study in which healthy men and women were randomized to treatment with intranasal OT or placebo. Afterwards, they were imaged with functional magnetic resonance imaging while playing an iterated Prisoner's Dilemma Game with same-sex partners. Within the left ventral caudate nucleus, intranasal OT treatment increased activation to reciprocated cooperation in men, but tended to decrease activation in women. Here, we show that these sex differences in OT effects are specific to individuals with the rs53576 GG genotype, and are not found for other genotypes (rs53576 AA/AG). Thus, OT may increase the reward or salience of positive social interactions for male GG homozygotes, while decreasing those processes for female GG homozygotes. These results suggest that rs53576 genotype is an important variable to consider in future investigations of the clinical efficacy of intranasal OT treatment. © 2015 John Wiley & Sons Ltd and International Behavioural and Neural Genetics Society.

Occupational therapy practice in emergency care: Occupational therapists' perspectives.

Science.gov (United States)

Spang, Lisa; Holmqvist, Kajsa

2015-01-01

Emergency care takes place in a complex work environment that is characterized by critically ill patients, short hospital stays, and a wide variety of different healthcare professionals. Studies of occupational therapists' (OTs) experiences of working within emergency care have shown that they often experience difficulties in explaining the essence of occupational therapy and have to justify their approaches. Much effort has been made in Sweden to help OTs dispel the notion that occupational therapy is difficult to explain, and the aim of this study was to describe how Swedish OTs perceive their work in emergency care. A qualitative descriptive approach was taken, and 14 interviews were conducted with OTs working in emergency care. Qualitative content analysis was used to analyse the data. The overall theme that emerged was "Feeling established through deliberate occupation-based work". The underlying categories showed different strategies used by the OTs to provide occupational therapy in an emergency care context. Deliberate strategies were used to demonstrate the effectiveness of occupational therapy and its approaches to patients and other health care professionals, and this resulted in the OTs feeling both established and needed. Unlike the OTs in previous studies, the Swedish OTs experienced no difficulties in explaining occupational therapy and could make convincing arguments for their interventions. Parallel to their clinical work, the OTs worked with on-going development to find ways to improve their approaches. In summary, these Swedish OTs seem to have been provided with a professional language and the knowledge required to establish themselves in an emergency care setting.

Sex differences in neural activation following different routes of oxytocin administration in awake adult rats.

Science.gov (United States)

Dumais, Kelly M; Kulkarni, Praveen P; Ferris, Craig F; Veenema, Alexa H

2017-07-01

The neuropeptide oxytocin (OT) regulates social behavior in sex-specific ways across species. OT has promising effects on alleviating social deficits in sex-biased neuropsychiatric disorders. However little is known about potential sexually dimorphic effects of OT on brain function. Using the rat as a model organism, we determined whether OT administered centrally or peripherally induces sex differences in brain activation. Functional magnetic resonance imaging was used to examine blood oxygen level-dependent (BOLD) signal intensity changes in the brains of awake rats during the 20min following intracerebroventricular (ICV; 1μg/5μl) or intraperitoneal (IP; 0.1mg/kg) OT administration as compared to baseline. ICV OT induced sex differences in BOLD activation in 26 out of 172 brain regions analyzed, with 20 regions showing a greater volume of activation in males (most notably the nucleus accumbens and insular cortex), and 6 regions showing a greater volume of activation in females (including the lateral and central amygdala). IP OT also elicited sex differences in BOLD activation with a greater volume of activation in males, but this activation was found in different and fewer (10) brain regions compared to ICV OT. In conclusion, exogenous OT modulates neural activation differently in male versus female rats with the pattern and magnitude, but not the direction, of sex differences depending on the route of administration. These findings highlight the need to include both sexes in basic and clinical studies to fully understand the role of OT on brain function. Copyright © 2017 Elsevier Ltd. All rights reserved.

A pilot study of rhythm and timing training as a supplement to occupational therapy in stroke rehabilitation.

Science.gov (United States)

Hill, Valerie; Dunn, Leah; Dunning, Kari; Page, Stephen J

2011-01-01

Stroke is the leading cause of disability. A need exists for an effective intervention to enhance upper extremity (UE) motor abilities and activities of daily living (ADL) performance. The objectives of this pilot study were to (1) determine the feasibility of adding Interactive Metronome (IM) to an occupational therapy (OT) program; and (2) determine changes in UE impairments, function, quality of life, and perceived physical performance ability and satisfaction using a combined IM + OT regimen compared with OT alone for adults with chronic stroke. This pilot study (n=10) used a 2-group (OT or IM+OT) pretest-posttest design. The intervention involved 60 minutes of IM + OT or OT alone, 3 days a week for 10 weeks. Outcome measures included the UE Fugl-Meyer (impairment), the Arm Motor Ability Test (function), the Box and Block Test (function), Stroke Impact Scale (quality of life), and the Canadian Occupational Performance Measure (perceived performance ability and satisfaction). It was feasible to add IM to OT. The IM+OT group demonstrated decreased impairment and increased quality of life. However, the OT-alone group demonstrated greater gains in function, perceived physical performance ability, and satisfaction. These findings suggest that rhythm and timing training using the IM is a feasible intervention to consider as part of therapy treatment. However, IM may fit best for prefunctional treatment, as it seemed to primarily decrease impairment. It may also serve as a supplement before or after treatment in order to maximize rehabilitation potential. Clinical implications and suggestions for future studies are provided.

75 FR 42833 - Prohibited Service at Savings and Loan Holding Companies

Science.gov (United States)

2010-07-22

....treas.gov . OTS will post comments and the related index on the OTS Internet Site at http://www.ots... regulations applicable to savings associations under Regulation DD. Regulation DD implements the Truth in...

Orthostatic Tremor and Orthostatic Myoclonus: Weight-bearing Hyperkinetic Disorders: A Systematic Review, New Insights, and Unresolved Questions

Directory of Open Access Journals (Sweden)

Anhar Hassan

2016-11-01

Full Text Available Background: Orthostatic tremor (OT and orthostatic myoclonus (OM are weight-bearing hyperkinetic movement disorders most commonly affecting older people that induce “shaky legs” upon standing. OT is divided into “classical” and “slow” forms based on tremor frequency. In this paper, the first joint review of OT and OM, we review the literature and compare and contrast their demographic, clinical, electrophysiological, neuroimaging, pathophysiological, and treatment characteristics. Methods: A PubMed search up to July 2016 using the phrases “orthostatic tremor,” “orthostatic myoclonus,” “shaky legs,” and “shaky legs syndrome” was performed. Results: OT and OM should be suspected in older patients reporting unsteadiness with prolonged standing and/or who exhibit cautious, wide-based gaits. Surface electromyography (SEMG is necessary to verify the diagnoses. Functional neuroimaging and electrophysiology suggest the generator of classical OT lies within the cerebellothalamocortical network. For OM, and possibly slow OT, the frontal, subcortical cerebrum is the most likely origin. Clonazepam is the most useful medication for classical OT, and levetiracetam for OM, although results are often disappointing. Deep brain stimulation appears promising for classical OT. Rolling walkers reliably improve gait affected by these disorders, as both OT and OM attenuate when weight is transferred from the legs to the arms. Discussion: Orthostatic hyperkinesias are likely underdiagnosed, as SEMG is often unavailable in clinical practice, and thus may be more frequent than currently recognized. The shared weight-bearing induction of OT and OM may indicate a common pathophysiology. Further research, including use of animal models, is necessary to better define the prevalence and pathophysiology of OT and OM, in order to improve their treatment, and provide additional insights into basic balance and gait mechanisms.

Cuban Ocular Toxoplasmosis Epidemiology Study (COTES): incidence and prevalence of ocular toxoplasmosis in Central Cuba.

Science.gov (United States)

Bustillo, Jorge L; Diaz, Jose D; Pacheco, Idarmes C; Gritz, David C

2015-03-01

Serological studies indicate that rates of ocular toxoplasmosis (OT) vary geographically, with higher rates in tropical regions. Little is known about population-based rates of active OT. We aimed to describe the epidemiology of OT in Central Cuba. This large-population, cross-sectional cohort study used a prospective database at a large regional referral centre in Central Cuba. The patient database was searched for all patients who presented with OT during the 12-month study period from 1 April 2011 to 31 March 2012. Inclusion criteria were the clinical diagnosis of OT, characterised by focal retinochoroidal inflammation and a response to therapy as expected. Gender-stratified and age-stratified study population data from the 2012 Cuban Census were used to calculate incidence rates and prevalence ratios. Among 279 identified patients with OT, 158 presented with active OT. Of these, 122 new-onset and 36 prior-onset cases were confirmed. Based on the total population in the Sancti Spiritus province (466,106 persons), the overall incidence of active OT was 26.2 per 100,000 person-years (95% CI 21.7 to 31.3) with an annual prevalence ratio of 33.9 per 100,000 persons (95% CI 28.8 to 39.6). The incidence of active OT was lowest in the oldest age group and highest in patients aged 25-44 years (4.5 and 42.1 per 100,000 person-years, respectively). This first report describing population-based rates of OT in the Cuban population highlights the importance of patient age as a likely risk factor for OT. Disease rates were found to be highest in females and young to middle-aged adults. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Oxytocin induces prostaglandin F2 alpha release in pregnant cows: influence of gestational age and oxytocin receptor concentrations.

Science.gov (United States)

Fuchs, A R; Rollyson, M K; Meyer, M; Fields, M J; Minix, J M; Randel, R D

1996-03-01

Brahman cows with known breeding dates received i.v. injections of either 10 or 100 IU oxytocin (OT) on Days 50, 150, 250, or 280 of gestation (n = 6 for each stage). Concentrations of the prostaglandin (PG) F2 alpha metabolite, 13,14-dihydro-15-keto-prostaglandin (PGFM), and OT were measured in samples of peripheral plasma collected at 15-min intervals for 1 h before and 1 h after treatment and then at 30-min intervals for 3 h. Plasma progesterone was measured daily for 14 days after OT injections on Days 50 and 250 of gestation. The increase in plasma OT after injection was dose-dependent (p = 0.001) but not affected by stage of gestation. Plasma PGFM increased after OT in a dose- and stage-dependent manner (p = 0.0001). At Day 280, the increase in plasma PGFM after 100 IU OT was sevenfold greater than at Day 50. Plasma progesterone declined significantly during the 7th to 12th days postinjection and returned to normal pregnancy values by the 14th day (4.4 +/- 0.3 ng/ml) except in two cows treated on Day 50 of gestation that later aborted. In these, plasma progesterone was significantly lower, 2.6 +/- 0.1 ng/ml. In a second experiment, the concentration of OT receptors was determined in endometrium collected from purebred Angus or Hereford cows slaughtered on Days 50, 150, 250, and 280 of gestation (n = 3 or 4 at each stage). Endometrial concentrations of OT receptor changed as a function of gestational age, increasing sixfold from Day 50 to Day 280, which was parallel to the increase by OT of plasma PGFM. Thus, endometrial OT receptors are functionally coupled to PGF2 alpha release during pregnancy, and their concentration determines the magnitude of OT-induced PGF2 alpha release during gestation. Consequently, endogenous OT is a factor in the regulation of PGF2 alpha release from the bovine uterus during pregnancy and parturition.

The effects of intranasal oxytocin on reward circuitry responses in children with autism spectrum disorder.

Science.gov (United States)

Greene, R K; Spanos, M; Alderman, C; Walsh, E; Bizzell, J; Mosner, M G; Kinard, J L; Stuber, G D; Chandrasekhar, T; Politte, L C; Sikich, L; Dichter, G S

2018-03-27

Intranasal oxytocin (OT) has been shown to improve social communication functioning of individuals with autism spectrum disorder (ASD) and, thus, has received considerable interest as a potential ASD therapeutic agent. Although preclinical research indicates that OT modulates the functional output of the mesocorticolimbic dopamine system that processes rewards, no clinical brain imaging study to date has examined the effects of OT on this system using a reward processing paradigm. To address this, we used an incentive delay task to examine the effects of a single dose of intranasal OT, versus placebo (PLC), on neural responses to social and nonsocial rewards in children with ASD. In this placebo-controlled double-blind study, 28 children and adolescents with ASD (age: M = 13.43 years, SD = 2.36) completed two fMRI scans, one after intranasal OT administration and one after PLC administration. During both scanning sessions, participants completed social and nonsocial incentive delay tasks. Task-based neural activation and connectivity were examined to assess the impact of OT relative to PLC on mesocorticolimbic brain responses to social and nonsocial reward anticipation and outcomes. Central analyses compared the OT and PLC conditions. During nonsocial reward anticipation, there was greater activation in the right nucleus accumbens (NAcc), left anterior cingulate cortex (ACC), bilateral orbital frontal cortex (OFC), left superior frontal cortex, and right frontal pole (FP) during the OT condition relative to PLC. Alternatively, during social reward anticipation and outcomes, there were no significant increases in brain activation during the OT condition relative to PLC. A Treatment Group × Reward Condition interaction revealed relatively greater activation in the right NAcc, right caudate nucleus, left ACC, and right OFC during nonsocial relative to social reward anticipation during the OT condition relative to PLC. Additionally, these analyses revealed

Radioimmunoassay of urine oxytocin in man

International Nuclear Information System (INIS)

Zebidi, A.; Geelen, G.; Allevard, A.M.; Sempore, B.; Jarsaillon, E.; Meunier, C.; Gharib, C.

1978-01-01

A radioimmunoassay (RIA) for oxytocin (OT) in urine is described. 125 I-OT was prepared, and antibodies were raised in rabbits against OT coupled to bovine serumalbumine. This allowed us to set up a RIA for OT which limit of detection is 1.25 pg/tube (0.6 μU). The use of an extraction procedure using CG 50 Amberlite is essential. The recovery after extraction reaches 70.5 %. pH 5 is the optimum pH were urine samples must be stored. The superposition of the elution peak of endogenous OT on that of exogenous hormone is an argument in favour of the validity of such an extraction procedure. Daily urinary excretion of OT reaches 9.58 mU +- 3.48 in 18 healthy young men [fr

Reformierakond ei kauple põhimõtetega / Meelis Atonen

Index Scriptorium Estoniae

Atonen, Meelis, 1966-

2001-01-01

Ilmunud ka: Hiiu Leht, 11. dets. 2001, lk. 2; Nädaline, 11. dets. 2001, lk. 4; Valgamaalane, 11. dets. 2001, lk. 2; Järva Teataja, 11. dets. 2001, lk. 2; Kesknädal, 19. dets. 2001, lk. 5. Tallinna võimuliidu lagunemise peapõhjus oli Tallinna 2002. aasta eelarvesse kavandatud 900 mln kuni 1,5 mld krooni suurune laen, millega Reformierakond ei ole kunagi nõustnud. Autor: Reformierakond. Parlamendisaadik

"Rohkem naisest kui näitlejast..." / Meelis Kapstas

Index Scriptorium Estoniae

Kapstas, Meelis, 1963-

2006-01-01

Ilmus Ita Everi elulooraamat "Ita Ever. Elu suuruses". Näitlejannad Viire Valdma, Mari-Liis Lill, Ülle Kaljuste, Britta Vahur, Elina Reinold, Kersti Kreismann, Merle Palmiste ja Ülle Ulla avaldavad oma esmamulje raamatust

Eluviisi määrab valikuvabadus / Meelis Press

Index Scriptorium Estoniae

Press, Meelis, 1965-

1998-01-01

Kodu loomine tähendab mõtteviisi. Erinevaid elamisvõimalusi maailmast : taotluslik looduslähedus (S. Fehni loodud kodu Norras), afisheeritud metropol, maja kui elav organism (T. Spiegelhalteri projekteeritud päikesepatareidega elamu), T. Fujimori projekteeritud võilillemaja Jaapanis, K. Masahashi plekkangaarist konstrueeritud talumaja jm.

Tulumaks 20 protsenti - julgust, sõbrad! / Meelis Atonen

Index Scriptorium Estoniae

Atonen, Meelis, 1966-

2002-01-01

Ilmunud ka: Molodjozh Estonii 31. mai lk. 5, Virumaa Teataja 30. mai lk. 7, Hiiumaa, Koit, Meie Maa 30. mai lk. 5,6,2, Sakala 30. mai lk. 2, Lääne-Harju Ekspress, Vooremaa 1. juuni lk. 4,8, Järva Teataja 7. juuni lk. 2, Pärnu Postimees 8. juuni lk. 14, Nädaline 15. juuni lk. 4, Valgamaalane 18. juuni lk. 2, Liberal-Forum juuni nr. 5 lk. 1, Hiiu Leht 26. juuni lk. 2. Reformierakonna ettepanek alandada üksikisiku tulumaksu 26% 20-le. Autor: Reformierakond. Parlamendisaadik

Aia alt pilvekatusega kunstisaali / Meeli Müüripeal

Index Scriptorium Estoniae

Müüripeal, Meeli, 1963-

2006-01-01

Sabile restaureeritud sünagoog-kunstisaalist (1890).1993. a. läti skulptori Ojars Arvids Feldbergsi (sünd. 1947) poolt avatud Pedvale keskkonnakunsti keskusest, sealsetest Brinkpedvale (18. sajand) ja Frikspedvale mõisahoonetest. 1999. a. pälvis Pedvale vabaõhu kunstisaal UNESCO Melina Mercouri auhinna. Pedvales on tuleetendusega esinenud Raul Meel, eesti kunstnike töödest nimetatud Villu Jaanisoo töö "Tugitool". 16 ill

Ameerikas keelekümblust õppimas / Meeli Parijõgi

Index Scriptorium Estoniae

Parijõgi, Meeli

2015-01-01

SA Innove keelekümbluskeskus viis veebruaris kahesuunalise keelekümbluse lasteaiaõpetajad nädalaks kogemusi omandama USA-sse Minnesota ülikooli. Kahesuunalist keelekümblust (pool päeva eesti ja pool vene keeles) hakatakse sügisest rakendama ka Eesti lasteaedades

Rosencrantz ja Guildenstern surevad Padise kloostris / Meelis Lainvoo

Index Scriptorium Estoniae

Lainvoo, Meelis

2005-01-01

Rakvere Teatri suvelavastus T. Stoppardi "Rosencrantz ja Guildenstern on jälle surnud" (lav. H. Toompere jun) mängitakse SA Virumaa Muuseumide poolt loa mittesaamise pärast Padise kloostri varemetel. Ka teatri suvelavastus "Pidusöök" on Rägavere mõisast üle kolinud Sagadi mõisa

Estimating absolute price level in Estonia / Meelis Angerma

Index Scriptorium Estoniae

Angerma, Meelis

2006-01-01

Lähema ühe kuni viie aasta jooksul asendab Eestis ühisraha euro kohaliku valuuta krooni, kaob võimalus arvutada Eesti-spetsiifilist reaalkurssi ehk hinnataset nominaalse vahetuskursi järgi. Suhtelise hinnataseme arvutamise vajadus EL-is kasvab, sest majandussidemed liikmesriikidega järjest tihenevad. Majandusotsuste langetamiseks vajavad majandussubjektid põhjalikku informatsiooni üksikute kaupade suhtelise hinna ja kaupade grupi suhtelise hinnataseme kohta. Tabelid

Kes on minu õunakest hammustanud? / Meelis A Piller

Index Scriptorium Estoniae

Piller, Meelis A

1998-01-01

Apple Macintosh tüüpi arvutist, mille tugevaim külg on värvikäsitlus ja mida eelistavad kasutada kujunduskunstnikud, professionaalsed reklaamifirmad, trükikojad, fotostuudiod. Suurimaks puuduseks on olnud odavamate koduarvutite puudumine.

Difficulties in organ procurement and transplantation in the aboriginal minority people in Taiwan.

Science.gov (United States)

Shih, F J; Chang, Y C; Chen, C F; Lin, F J; Huang, Y S; Yan, Y H; Shih, F Jong; Chen, C R

2012-05-01

Aboriginal people (AP) are a minority group in Taiwan. Little information on their perspectives on organ transplantation (OT) is available. Their rights for organ donation (OD) and as OT recipients (OTR) are constrained as a vulnerable population in society. This research sought to explore various Highland Aborigine Tribes beliefs systems and concepts related to OT. We employed a qualitative design on a purposive sample including seven categories of Taiwanese AP. Data collected by face-to-face interviews were evaluated by content analysis. Seventy-five informants (45 female and 30 males) of 18 to 82 years from seven tribes completed interviews: Bunun (n = 20), Shao (n = 18), Tsou (n = 15), Amis (n = 12), Truku (n = 4), Rukai (n = 3), and Puyuma (n = 3). Of there, 33% had no idea of OT. All informants reported lack of knowledge of OD, organ procurement, and OTR. Eighty percent (45-82 years) had no willingness for OD or OTR; others might consult family members and health professionals (HP) to learn about OT. Seven hindering factors were identified: (1) having no background of OT; (2) limited impressions obtained from television news reports; (3) negative concepts of donating one's organs to others; (4) OT concepts contrast with cultural meanings of death; (5) possibility of being stigmatized; (6) fear of being rejected by others; and (7) HP had never mentioned OT. Taiwan APs' perspectives of OT concepts showed the majority to be unfamiliar with the concept and benefits of OT. Future research is necessary to explore the possible avenues to facilitate communications between HP and AP leaders, as well as elders in each AP category in Taiwan. Copyright © 2012 Elsevier Inc. All rights reserved.

A conceptual-practice model for occupational therapy to facilitate return to work in breast cancer patients.

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Désiron, Huguette A M; Donceel, Peter; de Rijk, Angelique; Van Hoof, Elke

2013-12-01

Improved therapies and early detection have significantly increased the number of breast cancers survivors, leading to increasing needs regarding return to work (RTW). Occupational therapy (OT) interventions provide successful RTW assistance for other conditions, but are not validated in breast cancer. This paper aims to identify a theoretical framework for OT intervention by questioning how OT models can be used in OT interventions in RTW of breast cancer patients; criteria to be used to select these models and adaptations that would be necessary to match the OT model(s) to breast cancer patients' needs? Using research specific criteria derived from OT literature (conceptual OT-model, multidisciplinary, referring to the International Classification of functioning (ICF), RTW in breast cancer) a search in 9 electronic databases was conducted to select articles that describe conceptual OT models. A content analysis of those models complying to at least two of the selection criteria was realised. Checking for breast cancer specific issues, results were matched with literature of care-models regarding RTW in breast cancer. From the nine models initially identified, three [Canadian Model of Occupational Performance, Model of Human Occupation (MOHO), Person-Environment-Occupation-Performance model] were selected based on the selection criteria. The MOHO had the highest compliance rate with the criteria. To enhance usability in breast cancer, some adaptations are needed. No OT model to facilitate RTW in breast cancer could be identified, indicating a need to fill this gap. Individual and societal needs of breast cancer patients can be answered by using a MOHO-based OT model, extended with indications for better treatment, work-outcomes and longitudinal process factors.

Subcaste differences in neural activation suggest a prosocial role for oxytocin in eusocial naked mole-rats.

Science.gov (United States)

Hathaway, Georgia A; Faykoo-Martinez, Mariela; Peragine, Deane E; Mooney, Skyler J; Holmes, Melissa M

2016-03-01

The neuropeptide oxytocin (OT) influences prosocial behavior(s), aggression, and stress responsiveness, and these diverse effects are regulated in a species- and context-specific manner. The naked mole-rat (Heterocephalus glaber) is a unique species with which to study context-dependent effects of OT, exhibiting a strict social hierarchy with behavioral specialization within the subordinate caste: soldiers are aggressive and defend colonies against unfamiliar conspecifics while workers are prosocial and contribute to in-colony behaviors such as pup care. To determine if OT is involved in subcaste-specific behaviors, we compared behavioral responses between workers and soldiers of both sexes during a modified resident/intruder paradigm, and quantified activation of OT neurons in the hypothalamic paraventricular nucleus (PVN) and supraoptic nucleus (SON) using the immediate-early-gene marker c-fos co-localized with OT neurons. Resident workers and soldiers were age-matched with unfamiliar worker stimulus animals as intruders, and encounters were videorecorded and scored for aggressive behaviors. Colony-matched controls were left in their home colony for the duration of the encounters. Brains were extracted and cell counts were conducted for OT immunoreactive (ir), c-fos-ir, and percentage of OT-c-fos double-labeled cells. Results indicate that resident workers were less aggressive but showed greater OT neural activity than soldiers. Furthermore, a linear model including social treatment, cortisol, and subcaste revealed that subcaste was the only significant predictor of OT-c-fos double-labeled cells in the PVN. These data suggest that in naked mole-rats OT promotes prosocial behaviors rather than aggression and that even within subordinates status exerts robust effects on brain and behavior. Copyright © 2015 Elsevier Inc. All rights reserved.

The effects of oxytocin on fear recognition in patients with schizophrenia and in healthy controls

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Meytal eFischer-Shofty

2013-07-01

Full Text Available Individuals who suffer from schizophrenia often show a marked deficit in recognition of emotional facial expressions, as part of broader impairment of social cognition. Research has shown that recognition of negative emotions, specifically fear recognition, is particularly impaired among patients with schizophrenia. Recently we reported that intranasal administration of OT (IN OT increased the ability to correctly recognize fear in a group of healthy men. The aim of the current study was to examine the effects of IN OT administration on fear recognition among patients with schizophrenia. Based on previous research, we also sought to examine a possible selective effect of OT dependent on baseline performance, hypothesizing that IN OT would have a greater enhancement effect on less proficient individuals. It was thus hypothesized that patients will show more improvement in fear recognition following the administration of IN OT as compared to controls. Sixty six participants (31 schizophrenia patients, 35 healthy controls were enrolled in the current study. All participants received treatment of a single dose of 24 IU IN OT and an equivalent amount of placebo, one week apart. The participants’ ability to accurately recognize fear and happiness was evaluated using a face morphing task. Overall, as a group, both patients and healthy control participants were more accurate in recognizing fearful facial expressions, but not happy faces, following IN OT administration, as compared to their performance following placebo. IN OT did not differentially affect emotion recognition in patients and healthy controls. Yet, the results indicated a selective effect for IN OT, in which the hormone improves fear recognition only among individuals whose baseline performance was below the median, regardless of their psychiatric status.

Involvement of the oxytocin system in the bed nucleus of the stria terminalis in the sex-specific regulation of social recognition

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Dumais, Kelly M.; Alonso, Andrea G.; Immormino, Marisa A.; Bredewold, Remco; Veenema, Alexa H.

2015-01-01

Sex differences in the oxytocin (OT) system in the brain may explain why OT often regulates social behaviors in sex-specific ways. However, a link between sex differences in the OT system and sex-specific regulation of social behavior has not been tested. Here, we determined whether sex differences in the OT receptor (OTR) or in OT release in the posterior bed nucleus of the stria terminalis (pBNST) mediates sex-specific regulation of social recognition in rats. We recently showed that, compared to female rats, male rats have a three-fold higher OTR binding density in the pBNST, a sexually dimorphic area implicated in the regulation of social behaviors. We now demonstrate that OTR antagonist (5 ng/0.5 μl/side) administration into the pBNST impairs social recognition in both sexes, while OT (100 pg/0.5 μl/side) administration into the pBNST prolongs the duration of social recognition in males only. These effects seem specific to social recognition, as neither treatment altered total social investigation time in either sex. Moreover, baseline OT release in the pBNST, as measured with in vivo microdialysis, did not differ between the sexes. However, males showed higher OT release in the pBNST during social recognition compared to females. These findings suggest a sex-specific role of the OT system in the pBNST in the regulation of social recognition. PMID:26630388

Oxytocin and prolactin release after hypertonic saline administration in melatonin-treated male Syrian hamsters

International Nuclear Information System (INIS)

Juszczak, M.; Steger, R.W.; Fadden, C.; Bartke, A.

1996-01-01

The aim of the present investigations was to examine the effects of melatonin (Mel) on oxytocin (OT) release under conditions of osmotic stimulation, brought about by hypertonic saline administration, as well as to determine whether osmotically stimulated OT release in Mel-treated Syrian hamster is associated with alterations in the release of prolactin (PRL) and in norepinephrine (NE) and dopamine (DA) content in the hypothalamus. In both Mel- and vehicle-treated hamsters, injection of hypertonic saline was followed by a significant decrease in OT content in the pituitary neurointermediate lobe (NIL) and elevation of plasma OT and PRL levels. Melatonin injections had no significant affect on NIL OT content in either isotonic- or hypertonic-saline treated animals. Pretreatment with Mel did not alter plasma OT or PRL levels in isotonic saline-injected animals. However, Mel facilitated the release of OT, but prevented the release of PRL after hypertonic saline administration. Melatonin treatment reduced hypothalamic NE content (but not that of DA) in isotonic-saline treated animals. After osmotic stimulation, hypothalamic content of NE and DA was significantly lower in Mel-treated than in vehicle-treated animals. Data from the present study suggest that the osmotically-stimulated release of OT and PRL seems to be related to the activation of noradrenergic rather than dopaminergic transmission. Both dopaminergic and noradrenergic transmission may be, however, involved in mediating the effects of Mel on the osmotically-activated OT and PRL release. (author). 48 refs, 3 figs

Oxytocin and prolactin release after hypertonic saline administration in melatonin-treated male Syrian hamsters

Energy Technology Data Exchange (ETDEWEB)

Juszczak, M.; Steger, R.W.; Fadden, C.; Bartke, A. [Southern Illinois Univ., Carbondale, IL (United States)

1996-12-31

The aim of the present investigations was to examine the effects of melatonin (Mel) on oxytocin (OT) release under conditions of osmotic stimulation, brought about by hypertonic saline administration, as well as to determine whether osmotically stimulated OT release in Mel-treated Syrian hamster is associated with alterations in the release of prolactin (PRL) and in norepinephrine (NE) and dopamine (DA) content in the hypothalamus. In both Mel- and vehicle-treated hamsters, injection of hypertonic saline was followed by a significant decrease in OT content in the pituitary neurointermediate lobe (NIL) and elevation of plasma OT and PRL levels. Melatonin injections had no significant affect on NIL OT content in either isotonic- or hypertonic-saline treated animals. Pretreatment with Mel did not alter plasma OT or PRL levels in isotonic saline-injected animals. However, Mel facilitated the release of OT, but prevented the release of PRL after hypertonic saline administration. Melatonin treatment reduced hypothalamic NE content (but not that of DA) in isotonic-saline treated animals. After osmotic stimulation, hypothalamic content of NE and DA was significantly lower in Mel-treated than in vehicle-treated animals. Data from the present study suggest that the osmotically-stimulated release of OT and PRL seems to be related to the activation of noradrenergic rather than dopaminergic transmission. Both dopaminergic and noradrenergic transmission may be, however, involved in mediating the effects of Mel on the osmotically-activated OT and PRL release. (author). 48 refs, 3 figs.

Melanocortin Receptor Agonists Facilitate Oxytocin-Dependent Partner Preference Formation in the Prairie Vole.

Science.gov (United States)

Modi, Meera E; Inoue, Kiyoshi; Barrett, Catherine E; Kittelberger, Kara A; Smith, Daniel G; Landgraf, Rainer; Young, Larry J

2015-07-01

The central melanocortin (MC) system has been widely studied for its effects on food intake and sexual behavior. However, the MC system, and more specifically the MC4 receptor (MC4R), also interacts with neurochemical systems that regulate socioemotional behaviors, including oxytocin (OT) and dopamine. In monogamous prairie voles, OT and dopamine interact to promote partner preference formation, a laboratory measure of an enduring social bond between mates. Here we investigated the effects of MC receptor activation on partner preference formation in prairie voles, as well as the interaction between the MC and OT systems during this process. Peripheral administration of the brain penetrant MC3/4R receptor peptide agonist, Melanotan II (MTII), and the highly selective, small-molecule MC4R agonist, Pf-446687, enhanced partner preference formation in the prairie vole, but not in the non-monogamous meadow vole. MTII-induced partner preferences were enduring, as they were present 1 week after drug manipulation. The prosocial effects of MCR agonists may be mediated, in part, through modulation of OT, as coadministration of an OT receptor antagonist prevented MTII-induced partner preferences. MTII also selectively activated hypothalamic OT neurons and potentiated central OT release. As OT has been shown to enhance some aspects of social cognition in humans, our data suggest that the MC4R may be a viable therapeutic target for enhancing social function in psychiatric disorders, including autism spectrum disorders and schizophrenia, potentially through activation of the OT system.

Involvement of the oxytocin system in the bed nucleus of the stria terminalis in the sex-specific regulation of social recognition.

Science.gov (United States)

Dumais, Kelly M; Alonso, Andrea G; Immormino, Marisa A; Bredewold, Remco; Veenema, Alexa H

2016-02-01

Sex differences in the oxytocin (OT) system in the brain may explain why OT often regulates social behaviors in sex-specific ways. However, a link between sex differences in the OT system and sex-specific regulation of social behavior has not been tested. Here, we determined whether sex differences in the OT receptor (OTR) or in OT release in the posterior bed nucleus of the stria terminalis (pBNST) mediates sex-specific regulation of social recognition in rats. We recently showed that, compared to female rats, male rats have a three-fold higher OTR binding density in the pBNST, a sexually dimorphic area implicated in the regulation of social behaviors. We now demonstrate that OTR antagonist (5 ng/0.5 μl/side) administration into the pBNST impairs social recognition in both sexes, while OT (100 pg/0.5 μl/side) administration into the pBNST prolongs the duration of social recognition in males only. These effects seem specific to social recognition, as neither treatment altered total social investigation time in either sex. Moreover, baseline OT release in the pBNST, as measured with in vivo microdialysis, did not differ between the sexes. However, males showed higher OT release in the pBNST during social recognition compared to females. These findings suggest a sex-specific role of the OT system in the pBNST in the regulation of social recognition. Copyright © 2015 Elsevier Ltd. All rights reserved.

Occupational Therapy ’s Role in an Interprofessional Student-Run Free Clinic: Challenges and Opportunities Identified

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Oaklee Rogers

2017-07-01

Full Text Available Student-run free clinics (SRFCs provide services to underserved populations while enhancing student education. Occupational therapy (OT participation in integrated care SRFCs is an emerging area of practice and enhances the interprofessional model necessary for holistic patient care. The Student Health Outreach for Wellness (SHOW organization, located in Phoenix, Arizona, is a SRFC comprised of three state universities that incorporates nine different health professional programs to deliver interprofessional care, including OT. The SHOW clinic provides direct care services where student volunteers practice clinical and interprofessional skills under the guidance of licensed health care providers. OT preceptors and students participate in team-based assessment and care delivery to practice their discipline-specific treatment knowledge of upper-extremity impairments, musculoskeletal pain, medication management, fall prevention, and behavioral health issues, among many others. OT integration into the clinic provides students and preceptors an opportunity to build a unique skill set in interprofessional care, educate other disciplines about OT, and become emerging leaders in the field. Challenges for OT involvement in this SRFC include recruitment and sustainability of volunteers and lack of knowledge and understanding about the role of OT on an interprofessional team. Further research is needed to identify additional benefits of OT services in SFRCs.

MATERNAL MENTAL HEALTH MODERATES THE RELATIONSHIP BETWEEN OXYTOCIN AND INTERACTIVE BEHAVIOR.

Science.gov (United States)

Samuel, Simcha; Hayton, Barbara; Gold, Ian; Feeley, Nancy; Carter, C Sue; Zelkowitz, Phyllis

2015-01-01

Mothers with mood or anxiety disorders exhibit less optimal interactive behavior. The neuropeptide oxytocin (OT) has been linked to more optimal interactive behaviors in mothers without mental illness, and it may play a particularly beneficial role in mothers with mood or anxiety disorders given its antidepressant and anxiolytic functions. We compared the relationship between OT and interactive behavior in mothers with and without mental health problems. Participants included 20 women diagnosed with postpartum mood or anxiety disorders (clinical sample) and 90 women with low levels of depression and anxiety during pregnancy and postpartum (community sample). At 2 months' postpartum, blood was drawn to assess maternal OT levels, and mother-infant interaction was coded for maternal sensitivity, intrusiveness, remoteness, and depressiveness. Clinical mothers exhibited less sensitive, more intrusive, and more depressive interactive behaviors than did community mothers. The groups did not differ in OT levels. Mothers with higher OT levels were less intrusive with their infants. Higher OT levels were associated with less depressive interactive behavior only in clinical mothers. OT was associated with positive interactive behaviors in both groups. In clinical mothers, the calming and soothing effects of OT may promote more relaxed, energetic, and infant-focused interactive behaviors. © 2015 Michigan Association for Infant Mental Health.

Patient compliance with a health care provider referral for an occupational therapy lymphedema consult.

Science.gov (United States)

Dominick, Sally A; Natarajan, Loki; Pierce, John P; Madanat, Hala; Madlensky, Lisa

2014-07-01

Limited information exists on breast cancer patients' compliance to attend outpatient appointments with an occupational therapy (OT) lymphedema specialist. The objectives of this study were (1) to examine patient compliance with a health care provider referral for an OT lymphedema consult and (2) to identify potential barriers to compliance. A retrospective chart review of female breast cancer patients at the UC San Diego Health System was conducted. Electronic medical records were queried for breast cancer patients, who received a health care provider referral for an OT lymphedema consult between June 1, 2010 and December 31, 2011. Descriptive statistics and Fisher's exact chi-square tests were used to examine how specific participant characteristics were associated with attending an OT appointment. A total of 210 female patients received an OT referral from a health care provider related to their breast cancer diagnosis. Forty-three (20.5%) patients did not attend an OT appointment. Non-attenders were more likely to have had fewer lymph nodes removed (Pcancer patients attended recommended OT lymphedema consults, a substantial number of women might benefit from further education about OT for lymphedema prevention following breast cancer treatment. Further research to understand barriers to attendance is recommended, particularly among women with only sentinel nodes removed.

Failed Replication of Oxytocin Effects on Trust: The Envelope Task Case.

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Anthony Lane

Full Text Available The neurohormone Oxytocin (OT has been one of the most studied peptides in behavioral sciences over the past two decades. Many studies have suggested that OT could increase trusting behaviors. A previous study, based on the "Envelope Task" paradigm, where trust is assessed by the degree of openness of an envelope containing participant's confidential information, showed that OT increases trusting behavior and reported one of the most powerful effects of OT on a behavioral variable. In this paper we present two failed replications of this effect, despite sufficient power to replicate the original large effect. The non-significant results of these two failed replications clearly exclude a large effect of OT on trust in this paradigm but are compatible with either a null effect of OT on trust, or a small effect, undetectable with small sample size (N = 95 and 61 in Study 1 and 2, respectively. Taken together, our results question the purported size of OT's effect on trust and emphasize the need for replications.

(Mal adaptações metabólicas ao treinamento contínuo: concepções não consensuais de terminologia e diagnóstico Metabolic (mal adaptations to training continuum: misconceptions of terminology and diagnosis

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Franz Homero Paganini Burini

2010-10-01

Full Text Available Altos desempenhos esportivos demandam treinamentos pesados necessários ao estímulo adaptativo específico a cada esporte. A elevada carga de treino é geralmente acompanhada de discreta fadiga e reduções agudas no desempenho, mas caso acompanhada de períodos apropriados de recuperação, resulta em supercompensação metabólica ao treinamento, refletida como aumento na capacidade aeróbica e/ou força muscular. Visto como contínuo, os processos de intensificação do treinamento e o estresse relacionado à supercompensação, o aumento da sobrecarga ou do estresse poderá, em algum momento, acarretar a quebra da homeostase e a queda temporária da função (supra-alcance - OR ou supra-alcance funcional - FOR. Quando a sobrecarga excessiva de treinamento é combinada com recuperação inadequada há instalação do estado de supratreinamento (OT ou supra-alcance não funcional (NFOR. O OT excede o OR, cujo pico é também o limiar do OT resultando em desadaptações fisiológicas e queda crônica do desempenho físico. A forma crônica de desadaptação fisiológica ao treinamento físico é chamada de síndrome do supertreinamento (OTS. A própria expressão da síndrome denota a etiologia multifatorial do estado e reconhece que o exercício não é necessariamente seu único fator causal. O diagnóstico de OTS é baseado na recuperação ou não do desempenho. Não há biomarcador objetivo para OTS. A distinção entre OTS e NFOR (supratreinamento extremo é dependente de desfecho clínico e exclusão diagnóstica de doenças orgânicas, mais comuns na OTS. Também a diferença entre OR e OT é sutil e nenhum de seus marcadores bioquímicos pode ser universalizado. Não há evidências confirmatórias que OR evolui para OT ou que os sintomas de OT são piores dos que os de OR. Apenas pela fadiga aguda e queda de rendimento experimentada em sessões isoladas de treinamento, não é possível diferenciar presentemente os estados de OR e OT

Oxytocin is a cardiovascular hormone

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Gutkowska J.

2000-01-01

Full Text Available Oxytocin (OT, a nonapeptide, was the first hormone to have its biological activities established and chemical structure determined. It was believed that OT is released from hypothalamic nerve terminals of the posterior hypophysis into the circulation where it stimulates uterine contractions during parturition, and milk ejection during lactation. However, equivalent concentrations of OT were found in the male hypophysis, and similar stimuli of OT release were determined for both sexes, suggesting other physiological functions. Indeed, recent studies indicate that OT is involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of cardiovascular functions. It has long been known that OT induces natriuresis and causes a fall in mean arterial pressure, both after acute and chronic treatment, but the mechanism was not clear. The discovery of the natriuretic family shed new light on this matter. Atrial natriuretic peptide (ANP, a potent natriuretic and vasorelaxant hormone, originally isolated from rat atria, has been found at other sites, including the brain. Blood volume expansion causes ANP release that is believed to be important in the induction of natriuresis and diuresis, which in turn act to reduce the increase in blood volume. Neurohypophysectomy totally abolishes the ANP response to volume expansion. This indicates that one of the major hypophyseal peptides is responsible for ANP release. The role of ANP in OT-induced natriuresis was evaluated, and we hypothesized that the cardio-renal effects of OT are mediated by the release of ANP from the heart. To support this hypothesis, we have demonstrated the presence and synthesis of OT receptors in all heart compartments and the vasculature. The functionality of these receptors has been established by the ability of OT to induce ANP release from perfused heart or atrial slices. Furthermore, we have shown that the heart and large vessels

Comparing oxytocin and cortisol regulation in a double-blind, placebo-controlled, hydrocortisone challenge pilot study in children with autism and typical development.

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Corbett, Blythe A; Bales, Karen L; Swain, Deanna; Sanders, Kevin; Weinstein, Tamara A R; Muglia, Louis J

2016-01-01

Children with autism spectrum disorder (ASD) show marked impairment in social functioning and poor adaptation to new and changing contexts, which may be influenced by underlying regulatory processes. Oxytocin (OT) and cortisol are key neuromodulators of biological and behavioral responses, show a synergistic effect, and have been implicated in the neuropathological profile in ASD. However, they are rarely investigated together. The purpose of the pilot study was to evaluate the relationship between cortisol and OT in children with ASD under baseline and physiological stress (hydrocortisone challenge) conditions. Arginine vasopressin (AVP), structurally similar to OT, was also examined. A double-blind, placebo-controlled, randomly assigned, crossover design was employed in 25 children 8-to-12 years with ASD (N = 14) or typical development (TD, N = 11). A low dose of hydrocortisone and placebo were administered via liquid suspension. Analysis of variance (ANOVA) was used to examine the within-subject factor "Condition" (hydrocortisone/placebo) and "Time" (pre and post) and the between-subject factor "Group" (ASD vs. TD). Pearson correlations examined the relationship between hormone levels and clinical profile. There was a significant Time × Condition × Group interaction F (1.23) = 4.18, p = 0.05 showing a rise in OT during the experimental condition (hydrocortisone) and a drop during the placebo condition for the TD group but not the ASD group. There were no group differences for AVP. Hormone levels were associated with social profiles. For the TD group, an inverse relationship was observed. OT increased during physiological challenge suggesting that OT played a stress-buffering role during cortisol administration. In contrast for the ASD group, OT remained unchanged or decreased during both the physiological challenge and the placebo condition, suggesting that OT failed to serve as a stress buffer under conditions of physiological stress. While

Corticotropin-Releasing Factor Receptors Modulate Oxytocin Release in the Dorsolateral Bed Nucleus of the Stria Terminalis (BNST in Male Rats

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Daisy Martinon

2018-03-01

Full Text Available The neuropeptide oxytocin (OT plays an important role in the regulation of social and anxiety-like behavior. Our previous studies have shown that OT neurons send projections from the hypothalamus to the dorsolateral bed nucleus of the stria terminalis (BNSTdl, a forebrain region critically involved in the modulation of anxiety-like behavior. Importantly, these OT terminals in the BNSTdl express presynaptic corticotropin releasing factor (CRF receptor type 2 (CRFR2. This suggests that CRFR2 might be involved in the modulation of OT release. To test this hypothesis, we measured OT content in microdialysates collected from the BNSTdl of freely-moving male Sprague-Dawley rats following the administration of a selective CRFR2 agonist (Urocortin 3 or antagonist (Astressin 2B, As2B. To determine if type 1 CRF receptors (CRFR1 are also involved, we used selective CRFR1 antagonist (NBI35965 as well as CRF, a putative ligand of both CRFR1 and CRFR2. All compounds were delivered directly into the BNSTdl via reverse dialysis. OT content in the microdialysates was measured with highly sensitive and selective radioimmunoassay. Blocking CRFR2 with As2B caused an increase in OT content in BNSTdl microdialysates, whereas CRFR2 activation by Urocortin 3 did not have an effect. The As2B-induced increase in OT release was blocked by application of the CRFR1 antagonist demonstrating that the effect was dependent on CRFR1 transmission. Interestingly, CRF alone caused a delayed increase in OT content in BNSTdl microdialysates, which was dependent on CRF2 but not CRF1 receptors. Our results suggest that members of the CRF peptide family modulate OT release in the BNSTdl via a fine-tuned mechanism that involves both CRFR1 and CRFR2. Further exploration of mechanisms by which endogenous OT system is modulated by CRF peptide family is needed to better understand the role of these neuropeptides in the regulation of anxiety and the stress response.

Long-term exposure to intranasal oxytocin in a mouse autism model.

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Bales, K L; Solomon, M; Jacob, S; Crawley, J N; Silverman, J L; Larke, R H; Sahagun, E; Puhger, K R; Pride, M C; Mendoza, S P

2014-11-11

Oxytocin (OT) is a neuropeptide involved in mammalian social behavior. It is currently in clinical trials for the treatment of autism spectrum disorder (ASD). Previous studies in healthy rodents (prairie voles and C57BL/6J mice) have shown that there may be detrimental effects of long-term intranasal administration, raising the questions about safety and efficacy. To investigate the effects of OT on the aspects of ASD phenotype, we conducted the first study of chronic intranasal OT in a well-validated mouse model of autism, the BTBR T+ Itpr3tf/J inbred strain (BTBR), which displays low sociability and high repetitive behaviors. BTBR and C57BL/6J (B6) mice (N=94) were administered 0.8  IU/kg of OT intranasally, daily for 30 days, starting on day 21. We ran a well-characterized set of behavioral tasks relevant to diagnostic and associated symptoms of autism, including juvenile reciprocal social interactions, three-chambered social approach, open-field exploratory activity, repetitive self-grooming and fear-conditioned learning and memory, some during and some post treatment. Intranasal OT did not improve autism-relevant behaviors in BTBR, except for female sniffing in the three-chambered social interaction test. Male saline-treated BTBR mice showed increased interest in a novel mouse, both in chamber time and sniffing time, whereas OT-treated male BTBR mice showed a preference for the novel mouse in sniffing time only. No deleterious effects of OT were detected in either B6 or BTBR mice, except possibly for the lack of a preference for the novel mouse's chamber in OT-treated male BTBR mice. These results highlight the complexity inherent in understanding the effects of OT on behavior. Future investigations of chronic intranasal OT should include a wider dose range and early developmental time points in both healthy rodents and ASD models to affirm the efficacy and safety of OT.

Low dose intranasal oxytocin delivered with Breath Powered device dampens amygdala response to emotional stimuli: A peripheral effect-controlled within- subjects randomized dose-response fMRI trial

OpenAIRE

Quintana, Daniel; Westlye, Lars Tjelta; Alnæs, Dag; Rustan, Øyvind; Kaufmann, Tobias; Smerud, Knut Terje; Mahmoud, Ramy; Djupesland, Per G.; Andreassen, Ole Andreas

2016-01-01

It is unclear if and how exogenous oxytocin (OT) reaches the brain to improve social behavior and cognition and what is the optimal dose for OT response. To better understand the delivery routes of intranasal OT administration to the brain and the dose-response, we compared amygdala response to facial stimuli by means of functional magnetic resonance imaging (fMRI) in four treatment conditions, including two different doses of intranasal OT using a novel Breath Powered device, intravenous (IV...

Emotional suppression explains the link between early life stress and plasma oxytocin.

Science.gov (United States)

Mohiyeddini, Changiz; Opacka-Juffry, Jolanta; Gross, James J

2014-01-01

Early life stress (ELS) has been found to be associated with lower concentrations of plasma oxytocin (OT) in adulthood. It is not yet clear, however, what mechanisms underlie this association. The goal of the present study was to test the role of emotional suppression as an intervening variable between ELS in childhood and plasma OT. In a nonclinical sample of 90 men, ELS, emotional suppression, and plasma OT were assessed. Emotional suppression was positively associated with ELS (r = 0.37, p emotion regulation strategy - was not correlated with ELS or plasma OT concentrations. Cross-sectional regression analyses revealed that the ELS explained variance in plasma OT via emotional suppression. Moderation analyses revealed that the combination of high ELS and high emotional suppression was associated with the lowest concentrations of plasma oxytocin. These findings are consistent with the view that emotional suppression may be one pathway linking ELS and OT.

Content of conventional therapy for the severely affected arm during subacute rehabilitation after stroke: An analysis of physiotherapy and occupational therapy practice.

Science.gov (United States)

de Jong, Lex D; van Wijck, Frederike; Stewart, Roy E; Geurts, Alexander C H; Dijkstra, Pieter U

2018-01-01

Physiotherapy (PT) and occupational therapy (OT) are key professions providing treatment for the arm after stroke; however, knowledge about the content of these treatments is scant. Detailed data are needed to replicate interventions, evaluate their effective components, and evaluate PT and OT practice. This paper describes PT and OT treatment for the severely affected arm in terms of duration, content according to components and categories of the International Classification of Human Functioning, Disability and Health, and to analyze differences between professions. Design: This is a retrospective analysis of randomized trial data. 46 patients after stroke with poor arm motor control recruited from inpatient neurological units from three rehabilitation centers in the Netherlands. PTs and OTs recorded duration and content of arm treatment interventions for 8 weeks using a bespoke treatment schedule with 15 International Classification of Human Functioning, Disability and Health categories. PTs and OTs spent on average 4-7 min per treatment session (30 min) on arm treatment. OTs spent significantly more time providing arm treatment and treatment at the activities level than PTs. PTs spent 79% of their arm treatment time on body functions, OTs 41%. OTs spent significantly more time on "moving around using transportation," "self care," and "household tasks" categories. Patients after stroke with a severely affected arm and an unfavorable prognosis for arm motor recovery receive little arm-oriented PT and OT. Therapists spent most arm treatment time on body functions. There was a considerable overlap in the content of PT and OT in 12 of the 15 categories. Results can be generalized only to patients with poor arm motor control and may not represent practice in other countries. Copyright © 2017 John Wiley & Sons, Ltd.

Kinetic and Structural Characterization of a Heterohexamer 4-Oxalocrotonate Tautomerase from Chloroflexus aurantiacus J-10-fl: Implications for Functional and Structural Diversity in the Tautomerase Superfamily

International Nuclear Information System (INIS)

Burks, Elizabeth A.; Fleming, Christopher D.; Mesecar, Andrew D.; Whitman, Christian P.; Pegan, Scott D.

2010-01-01

4-Oxalocrotonate tautomerase (4-OT) isozymes play prominent roles in the bacterial utilization of aromatic hydrocarbons as sole carbon sources. These enzymes catalyze the conversion of 2-hydroxy-2,4-hexadienedioate (or 2-hydroxymuconate) to 2-oxo-3-hexenedioate, where Pro-1 functions as a general base and shuttles a proton from the 2-hydroxyl group of the substrate to the C-5 position of the product. 4-OT, a homohexamer from Pseudomonas putida mt-2, is the most extensively studied 4-OT isozyme and the founding member of the tautomerase superfamily. A search of five thermophilic bacterial genomes identified a coded amino acid sequence in each that had been annotated as a tautomerase-like protein but lacked Pro-1. However, a nearby sequence has Pro-1, but the sequence is not annotated as a tautomerase-like protein. To characterize this group of proteins, two genes from Chloroflexus aurantiacus J-10-fl were cloned, and the corresponding proteins were expressed. Kinetic, biochemical, and X-ray structural analyses show that the two expressed proteins form a functional heterohexamer 4-OT (hh4-OT), composed of three αβ dimers. Like the P. putida enzyme, hh4-OT requires the amino-terminal proline and two arginines for the conversion of 2-hydroxymuconate to the product, implicating an analogous mechanism. In contrast to 4-OT, hh4-OT does not exhibit the low-level activity of another tautomerase superfamily member, the heterohexamer trans-3-chloroacrylic acid dehalogenase (CaaD). Characterization of hh4-OT enables functional assignment of the related enzymes, highlights the diverse ways the β-α-β building block can be assembled into an active enzyme, and provides further insight into the molecular basis of the low-level CaaD activity in 4-OT.

Developmental changes in hypothalamic oxytocin and oxytocin receptor mRNA expression and their sensitivity to fasting in male and female rats.

Science.gov (United States)

Matsuzaki, Toshiya; Iwasa, Takeshi; Munkhzaya, Munkhsaikhan; Tungalagsuvd, Altankhuu; Kawami, Takako; Murakami, Masahiro; Yamasaki, Mikio; Yamamoto, Yuri; Kato, Takeshi; Kuwahara, Akira; Yasui, Toshiyuki; Irahara, Minoru

2015-04-01

Oxytocin (OT) affects the central nervous system and is involved in a variety of social and non-social behaviors. Recently, the role played by OT in energy metabolism and its organizational effects on estrogen receptor alpha (ER-α) during the neonatal period have gained attention. In this study, the developmental changes in the hypothalamic mRNA levels of OT, the OT receptor (OTR), and ER-α were evaluated in male and female rats. In addition, the fasting-induced changes in the hypothalamic mRNA levels of OT and the OTR were evaluated. Hypothalamic explants were taken from postnatal day (PND) 10, 20, and 30 rats, and the mRNA level of each molecule was measured. Hypothalamic OT mRNA expression increased throughout the developmental period in both sexes. The rats' hypothalamic OTR mRNA levels were highest on PND 10 and decreased throughout the developmental period. In the male rats, the hypothalamic mRNA levels of ER-α were higher on PND 30 than on PND 10. On the other hand, no significant differences in hypothalamic ER-α mRNA expression were detected among the examined time points in the female rats, although hypothalamic ER-α mRNA expression tended to be higher on PND 30 than on PND 10. Significant positive correlations were detected between hypothalamic OT and ER-α mRNA expression in both the male and female rats. Hypothalamic OT mRNA expression was not affected by fasting at any of the examined time points in either sex. These results indicate that hypothalamic OT expression is not sensitive to fasting during the developmental period. In addition, as a positive correlation was detected between hypothalamic OT and ER-α mRNA expression, these two molecules might interact with each other to induce appropriate neuronal development. Copyright © 2015 Elsevier Ltd. All rights reserved.

Toward the Decision Tree for Inferring Requirements Maturation Types

Science.gov (United States)

Nakatani, Takako; Kondo, Narihito; Shirogane, Junko; Kaiya, Haruhiko; Hori, Shozo; Katamine, Keiichi

Requirements are elicited step by step during the requirements engineering (RE) process. However, some types of requirements are elicited completely after the scheduled requirements elicitation process is finished. Such a situation is regarded as problematic situation. In our study, the difficulties of eliciting various kinds of requirements is observed by components. We refer to the components as observation targets (OTs) and introduce the word “Requirements maturation.” It means when and how requirements are elicited completely in the project. The requirements maturation is discussed on physical and logical OTs. OTs Viewed from a logical viewpoint are called logical OTs, e.g. quality requirements. The requirements of physical OTs, e.g., modules, components, subsystems, etc., includes functional and non-functional requirements. They are influenced by their requesters' environmental changes, as well as developers' technical changes. In order to infer the requirements maturation period of each OT, we need to know how much these factors influence the OTs' requirements maturation. According to the observation of actual past projects, we defined the PRINCE (Pre Requirements Intelligence Net Consideration and Evaluation) model. It aims to guide developers in their observation of the requirements maturation of OTs. We quantitatively analyzed the actual cases with their requirements elicitation process and extracted essential factors that influence the requirements maturation. The results of interviews of project managers are analyzed by WEKA, a data mining system, from which the decision tree was derived. This paper introduces the PRINCE model and the category of logical OTs to be observed. The decision tree that helps developers infer the maturation type of an OT is also described. We evaluate the tree through real projects and discuss its ability to infer the requirements maturation types.

Olfactory tubercle stimulation alters odor preference behavior and recruits forebrain reward and motivational centers

Directory of Open Access Journals (Sweden)

Brynn J FitzGerald

2014-03-01

Full Text Available Rodents show robust behavioral responses to odors, including strong preferences or aversions for certain odors. The neural mechanisms underlying the effects of odors on these behaviors in animals are not well understood. Here, we provide an initial proof-of-concept study into the role of the olfactory tubercle (OT, a structure with known anatomical connectivity with both brain reward and olfactory structures, in regulating odor-motivated behaviors. We implanted c57bl/6 male mice with an ipsilateral bipolar electrode into the OT to administer electric current and thereby yield gross activation of the OT. We confirmed that electrical stimulation of the OT was rewarding, with mice frequently self-administering stimulation on a fixed ratio schedule. In a separate experiment, mice were presented with either fox urine or peanut odors in a three-chamber preference test. In absence of OT stimulation, significant preference for the peanut odor chamber was observed which was abolished in the presence of OT stimulation. Perhaps providing a foundation for this modulation in behavior, we found that OT stimulation significantly increased the number of c-Fos positive neurons in not only the OT, but also in forebrain structures essential to motivated behaviors, including the nucleus accumbens and lateral septum. The present results support the notion that the OT is integral to the display of motivated behavior and possesses the capacity to modulate odor hedonics either by directly altering odor processing or perhaps by indirect actions on brain reward and motivation structures.

Natural variations in maternal and paternal care are associated with systematic changes in oxytocin following parent-infant contact.

Science.gov (United States)

Feldman, Ruth; Gordon, Ilanit; Schneiderman, Inna; Weisman, Omri; Zagoory-Sharon, Orna

2010-09-01

Animal studies have demonstrated that the neuropeptide oxytocin (OT) plays a critical role in processes of parent-infant bonding through mechanisms of early parental care, particularly maternal grooming and contact. Yet, the involvement of OT in human parenting remains poorly understood, no data are available on the role of OT in the development of human fathering, and the links between patterns of parental care and the OT response have not been explored in humans. One hundred and twelve mothers and fathers engaged in a 15-min play-and-contact interaction with their 4-6-month-old infants and interactions were micro-coded for patterns of parental touch. Results showed that baseline levels of plasma and salivary OT in mothers and fathers were similar, OT levels in plasma and saliva were inter-related, and OT was associated with the parent-specific mode of tactile contact. Human mothers who provided high levels of affectionate contact showed an OT increase following mother-infant interaction but such increase was not observed among mothers displaying low levels of affectionate contact. Among fathers, only those exhibiting high levels of stimulatory contact showed an OT increase. These results demonstrate consistency in the neuroendocrine basis of human parental interactions with those seen in other mammals. The findings underscore the need to provide opportunities for paternal care to trigger the biological basis of fatherhood and suggest that interventions that permit social engagement may be recommended in conditions of diminished maternal-infant contact, such as prematurity or postpartum depression. Copyright 2010 Elsevier Ltd. All rights reserved.

Binding characteristics of arylamine, o-toluidine, in rodent blood proteins, hemoglobin and albumin

International Nuclear Information System (INIS)

DeBord, D.G.; Swearengin, T.F.; Booth-Jones, A.B.; Wissinger, L.A.

1991-01-01

o-Toluidine (OT), an arylamine used as an anti-oxidant in the tire and rubber industry, is a suspect human carcinogen. In order to develop a method for biological monitoring of OT, the authors have examined the binding characteristics of OT to the blood proteins, albumin (Alb) and hemoglobin (Hb). Male Sprague-Dawley rats were treated with 10, 20, 40, 50, or 100 mg/kg [ 14 C0]-OT ip and terminated at 2, 4, 8, 18, or 24 hrs. Alb and Hb were isolated from the blood. OT binding was determined by radioactivity associated with Alb and Hb. Maximum OT binding occurred at 4 hrs for Alb and 24 hrs for Hb. The radioactivity associated with Alb was 2x greater than that associated with Hb at 4 hrs, and approximately 30% greater at 24 hrs. Similar OT binding to Alb was observed at the 50 and 100 mg/kg doses, while for Hb maximum binding was seen at 100 mg/kg. Extended time points were studied to determine which blood protein would be more useful for biological monitoring purposes. Rats were treated with 100 mg/kg bw [ 14 C]-OT and terminated at 1, 2 and 4 wks. The remaining radioactivity associated with Alb was 12% after 1 wk, and < 2% after 2 and 4 wks. Conversely, the radioactivity associated with Hb was 64% after 1 wk, 52% by 2 wks, and 24% by 4 wks. These results suggest that Hb may be more useful than Alb as a biological monitoring tool for OT

Scope of practice and supervision of orthodontic therapists in the United Kingdom: Part 2: a national cross-sectional survey of orthodontic therapists.

Science.gov (United States)

Ahmed, Farooq; Dugdale, Charlotte; Malik, Ovais; Waring, David

2018-03-01

Orthodontic therapists (OTs) are the most recent addition to the orthodontic clinical team. The General Dental Council (GDC) and the British Orthodontic Society have formulated guidance and guidelines relating to their scope of practice and level of supervision, however there has been no contemporary UK-based research investigating practice and supervision of OTs. The aim of this study was to investigate the scope of practice and level of supervision of OTs working in the UK. Ethical approval was received from the University of Manchester Research Ethics Committee. An anonymous postal questionnaire was dispatched using postal details acquired through the British Orthodontic Societies mailing list. Three mailings of the questionnaire were conducted. A 74% response rate was achieved. OTs routinely conducted 16 of the 20 procedures from their scope of practice. Uncommon procedures included fitting headgear (24%), lingual appliances (27%), inserting or removing temporary anchorage devices (20%), and taking facebow record (18%). A total of 62% of OTs took patient consent for treatment. 59% were supervised through a written prescription with no direct supervision. OTs were directly supervised for only a quarter of their clinical practice. Orthodontists viewing frequency for OTs varied significantly, and was found to be the following: every 2-4 visits (36%), every other visit (35%), and every visit (26%). OTs mostly carried out the scope of practice as permitted by the GDC. Procedures uncommon to routine orthodontic practice were also uncommon to Orthodontic therapist clinical practice. OTs work mostly through written prescription with no direct supervision.

Oxytocin and Socioemotional Aging─Current Knowledge and Future Trends

Directory of Open Access Journals (Sweden)

Natalie C. Ebner

2013-08-01

Full Text Available The oxytocin (OT system is involved in various aspects of social cognition and prosocial behavior. Specifically, OT has been examined in the context of social memory, emotion recognition, cooperation, trust, empathy, and bonding, and─though evidence is somewhat mixed─intranasal OT appears to benefit aspects of socioemotional functioning. However, most of the extant data on aging and OT is from animal research and human OT research has focused largely on young adults. As such, though we know that various socioemotional capacities change with age, we know little about whether age-related changes in the OT system may underlie age-related differences in socioemotional functioning. In this review, we take a genetic-neuro-behavioral approach and evaluate current evidence on age-related changes in the OT system as well as the putative effects of these alterations on age-related socioemotional functioning. Looking forward, we identify informational gaps and propose an Age-Related Genetic, Neurobiological, Sociobehavioral Model of Oxytocin (AGeNeS-OT model which may structure and inform investigations into aging-related genetic, neural, and sociocognitive processes related to OT. As an exemplar of the use of the model, we report exploratory data suggesting differences in socioemotional processing associated with genetic variation in the oxytocin receptor gene (OXTR in samples of young and older adults. Information gained from this arena has translational potential in depression, social stress, and anxiety─all of which have high relevance in aging─and may contribute to reducing social isolation and improving well-being of individuals across the lifespan.

Circulating tumor cells in melanoma patients.

Directory of Open Access Journals (Sweden)

Gary A Clawson

Full Text Available Circulating tumor cells (CTCs are of recognized importance for diagnosis and prognosis of cancer patients. With melanoma, most studies do not show any clear relationship between CTC levels and stage of disease. Here, CTCs were enriched (∼400X from blood of melanoma patients using a simple centrifugation device (OncoQuick, and 4 melanocyte target RNAs (TYR, MLANA, MITF, and MIF were quantified using QPCR. Approximately one-third of melanoma patients had elevated MIF and MLANA transcripts (p<0.0001 and p<0.001, respectively compared with healthy controls. In contrast, healthy controls had uniformly higher levels of TYR and MITF than melanoma patients (p<0.0001. There was a marked shift of leukocytes into the CTC-enriched fractions (a 430% increase in RNA recovery, p<0.001, and no relationship between CTC levels and stage of disease was found. CTCs were captured on microfabricated filters and cultured. Captured melanoma CTCs were large cells, and consisted of 2 subpopulations, based on immunoreactivity. One subpopulation (∼50% stained for both pan-cytokeratin (KRT markers and the common leukocyte marker CD-45, whereas the second subpopulation stained for only KRT. Since similar cells are described in many cancers, we also examined blood from colorectal and pancreatic cancer patients. We observed analogous results, with most captured CTCs staining for both CD-45/KRT markers (and for the monocyte differentiation marker CD-14. Our results suggest that immature melanocyte-related cells (expressing TYR and MITF RNA may circulate in healthy controls, although they are not readily detectable without considerable enrichment. Further, as early-stage melanomas develop, immature melanocyte migration into the blood is somehow curtailed, whereas a significant proportion of patients develop elevated CTC levels (based on MIF and MLANA RNAs. The nature of the captured CTCs is consistent with literature describing leukocyte/macrophage-tumor cell fusion hybrids

Tofacitinib attenuates pathologic immune pathways in patients with psoriasis: A randomized phase 2 study.

Science.gov (United States)

Krueger, James; Clark, James D; Suárez-Fariñas, Mayte; Fuentes-Duculan, Judilyn; Cueto, Inna; Wang, Claire Q; Tan, Huaming; Wolk, Robert; Rottinghaus, Scott T; Whitley, Maryann Z; Valdez, Hernan; von Schack, David; O'Neil, Shawn P; Reddy, Padmalatha S; Tatulych, Svitlana

2016-04-01

Tofacitinib is an oral Janus kinase inhibitor being investigated for psoriasis. We sought to elucidate the molecular mechanisms underlying the clinical efficacy of tofacitinib in patients with psoriasis. Twelve patients with plaque psoriasis were randomized (3:1) to receive 10 mg of tofacitinib or placebo twice daily for 12 weeks. Biopsy specimens were taken from nonlesional (baseline) and lesional (baseline, days 1 and 3, and weeks 1, 2, 4, and 12) skin. Biopsy specimens were examined for psoriatic epidermal features (thickness, Ki67(+) keratinocytes and keratin 16 [KRT16] mRNA expression, and phosphorylated signal transducer and activator of transcription [pSTAT](+) nuclei) and T-cell and dendritic cell (DC) subsets by using immunohistochemistry, and mRNA transcripts were quantified by using a microarray. In lesional skin keratinocyte pSTAT1 and pSTAT3 staining was increased at baseline but reduced after 1 day of tofacitinib (baseline, median of 1290 pSTAT1(+) cells/μm(2); day 1, median of 332 pSTAT1(+) cells/μm(2); and nonlesional, median of 155 pSTAT1(+) cells/μm(2)). Epidermal thickness and KRT16 mRNA expression were significantly and progressively reduced after days 1 and 3 of tofacitinib administration, respectively (eg, KRT16 decreased 2.74-fold, day 3 vs baseline, P = .016). Decreases in DC and T-cell numbers were observed after weeks 1 and 2, respectively. At week 4, significant decreases in IL-23/TH17 pathways were observed that persisted through week 12. Improvements in clinical and histologic features were strongly associated with changes in expression of psoriasis-related genes and reduction in IL-17 gene expression. Tofacitinib has a multitiered response in patients with psoriasis: (1) rapid attenuation of keratinocyte Janus kinase/STAT signaling; (2) removal of keratinocyte-induced cytokine signaling, leading to reductions in pathologic DC and T-cell numbers to nonlesional levels; and (3) inhibition of the IL-23/TH17 pathway. Copyright © 2016

Intranasal oxytocin modulates neural functional connectivity during human social interaction.

Science.gov (United States)

Rilling, James K; Chen, Xiangchuan; Chen, Xu; Haroon, Ebrahim

2018-02-10

Oxytocin (OT) modulates social behavior in primates and many other vertebrate species. Studies in non-primate animals have demonstrated that, in addition to influencing activity within individual brain areas, OT influences functional connectivity across networks of areas involved in social behavior. Previously, we used fMRI to image brain function in human subjects during a dyadic social interaction task following administration of either intranasal oxytocin (INOT) or placebo, and analyzed the data with a standard general linear model. Here, we conduct an extensive re-analysis of these data to explore how OT modulates functional connectivity across a neural network that animal studies implicate in social behavior. OT induced widespread increases in functional connectivity in response to positive social interactions among men and widespread decreases in functional connectivity in response to negative social interactions among women. Nucleus basalis of Meynert, an important regulator of selective attention and motivation with a particularly high density of OT receptors, had the largest number of OT-modulated connections. Regions known to receive mesolimbic dopamine projections such as the nucleus accumbens and lateral septum were also hubs for OT effects on functional connectivity. Our results suggest that the neural mechanism by which OT influences primate social cognition may include changes in patterns of activity across neural networks that regulate social behavior in other animals. © 2018 Wiley Periodicals, Inc.

Organotins in Neuronal Damage, Brain Function, and Behavior: A Short Review

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Igor Ferraz da Silva

2018-01-01

Full Text Available The consequences of exposure to environmental contaminants have shown significant effects on brain function and behavior in different experimental models. The endocrine-disrupting chemicals (EDC present various classes of pollutants with potential neurotoxic actions, such as organotins (OTs. OTs have received special attention due to their toxic effects on the central nervous system, leading to abnormal mammalian neuroendocrine axis function. OTs are organometallic pollutants with a tin atom bound to one or more carbon atoms. OT exposure may occur through the food chain and/or contaminated water, since they have multiple applications in industry and agriculture. In addition, OTs have been used with few legal restrictions in the last decades, despite being highly toxic. In addition to their action as EDC, OTs can also cross the blood–brain barrier and show relevant neurotoxic effects, as observed in several animal model studies specifically involving the development of neurodegenerative processes, neuroinflammation, and oxidative stress. Thus, the aim of this short review is to summarize the toxic effects of the most common OT compounds, such as trimethyltin, tributyltin, triethyltin, and triphenyltin, on the brain with a focus on neuronal damage as a result of oxidative stress and neuroinflammation. We also aim to present evidence for the disruption of behavioral functions, neurotransmitters, and neuroendocrine pathways caused by OTs.

A New Observing Tool for the James Clerk Maxwell Telescope

Science.gov (United States)

Folger, Martin; Bridger, Alan; Dent, Bill; Kelly, Dennis; Adamson, Andy; Economou, Frossie; Hirst, Paul; Jenness, Tim

A new Observing Tool (OT) has been developed at the UK Astronomy Technology Centre, Edinburgh, UK and the Joint Astronomy Centre, Hilo, Hawaii, USA. It is based on the Gemini Observing Tool and provides the first graphical observation preparation tool for the James Clerk Maxwell Telescope (JCMT) as well as being the first use of the OT for a non-optical/IR telescope. The OT allows the observer to assemble high level Science Programs using graphical representations of observation components such as instrument, target, and filter. This is later translated into low level control sequences for telescope and instruments. The new OT is designed to work on multiple telescopes: currently the UK Infrared Telescope (UKIRT) and JCMT. Object-oriented design makes the inclusion of telescope and instrument specific packages easy. The OT is written in Java using GUI packages such as Swing and JSky. A new component for the JCMT OT is the graphical Frequency Editor for Heterodyne instruments. It can be used to specify parameters such as frequencies, bandwidths, and sidebands of multiple subsystems, while graphically displaying the front-end frequency, emission lines and atmospheric transmission. In addition, Flexible Scheduling support has been added to the OT. The observer can define scheduling constraints by arranging observations graphically. Science Programs can be saved as XML or sent directly from the OT to a database (via SOAP).

OSD’s Obligation & Expenditure Rate Goals: An Examination of the Factors Contributing to the Interference

Science.gov (United States)

2015-04-30

Prepamg OAE leYd t-end dec$01𔃻 ~ 4 15 218 F 15 lack ot dec$01𔃻 ~at expected~ ~ 4 22 252 f 18 ot new OSO /SeMce poky 4~ 4 20 2 59 F17 directed POM 4...tacwed ~ 357 223 F35 Wehhold 35, 358 234 I F3S oa.IA edmnsVlltNe ktoons 35, 3 42 236 F37 RedorecbOt’t ot contr.cw efforts 35, 3 47 223 F38 OSO

Increasing the Impact of High-Resolution Lidar Topography Through Online Data Access and Processing

Science.gov (United States)

Crosby, C. J.; Nandigam, V.; Baru, C.; Arrowsmith, R.

2013-12-01

Topography data acquired with lidar (light detection and ranging) technology are revolutionizing the way we study the Earth's surface and overlying vegetation. These data, collected from satellite, airborne, tripod, or mobile-mounted scanners have emerged as a fundamental tool for research on topics including earthquake hazards, hillslope processes, and cyrosphere change. The U.S. National Science Foundation-funded OpenTopography (OT) Facility (http://www.opentopography.org) is a web-based system designed to democratize access to earth science-oriented lidar topography data. OT provides free, online access to lidar data in a number of forms, including the point cloud and associated geospatial-processing tools for customized analysis. The point cloud data are co-located with on-demand processing tools to generate digital elevation models, and derived products and visualizations which allow users to quickly access data in a format appropriate for their scientific application. The OT system is built using a service-oriented architecture (SOA) that leverages cyberinfrastructure resources at the San Diego Supercomputer Center at the University of California San Diego to allow users, regardless of expertise, to access these massive lidar datasets and derived raster data products for use in research and teaching. OT hosts over 600 billion lidar returns covering more than 120,000 km2. These data are provided by a variety of partners under joint agreements and memoranda of understanding with OT. Partners include national facilities such as the NSF-funded National Center for Airborne Lidar Mapping (NCALM), as well as non-governmental organizations and local, state, and federal agencies. OT has become a hub for high-resolution topography resources. Datasets hosted by other organizations, as well as lidar-specific software, can be registered into the OT catalog, providing users a 'one-stop shop' for such information. OT is also a partner on the NASA Lidar Access System (NLAS

32 CFR 3.4 - Definitions.

Science.gov (United States)

2010-07-01

... responsible for approving the OTs acquisition strategy and resulting OT agreement. This official must be at least one level above the Agreements Officer and at no lower level than existing agency thresholds associated with procurement contracts. Awardee. Any business unit that is the direct recipient of an OT...

77 FR 20104 - Privacy Act of 1974, as Amended; System of Records

Science.gov (United States)

2012-04-03

... Locator File OTS .012--Payroll/Personnel Systems & Payroll Records OTS .013--Mass Communication System OTS... examination or investigation; (3) The news media in accordance with guidelines contained in 28 CFR 50.2; (4... Information Act Officer, Communications Division, Mailstop 2-3, Office of the Comptroller of the Currency, 250...

REGULATION OF THE RAT OXYTOCIN GENE BY ESTRADIOL - EXAMINATION OF PROMOTER ACTIVITY IN TRANSFECTED CELLS AND OF MESSENGER-RIBONUCLEIC-ACID AND PEPTIDE LEVELS IN THE HYPOTHALAMONEUROHYPOPHYSEAL SYSTEM

NARCIS (Netherlands)

BURBACH, JPH; ADAN, RAH; VANTOL, HHM; VERBEECK, MAE; AXELSON, JF; VANLEEUWEN, FW; BEEKMAN, JM; AB, G

1990-01-01

Oxytocin (OT) plays a role in reproduction at the level of the pituitary and mammary glands and uterus. This OT is synthesized in the hypothalamo-neurohypophyseal system (HNS). A number of observations have suggested that estrogens regulate the production of OT in the HNS. In this study the effect

Comparison of Oral, Tympanic and Fresh Urine Temperature in ...

African Journals Online (AJOL)

Background: Core body temperature (CBT) is commonly evaluated by measuring oral (OT) and tympanic membrane (TT) temperatures; however, there are considerable debates on which of these methods is the most appropriate. Objective: To evaluate concordance of OT and TT with CBT. Material and Methods: OT, TT and ...

Men perform comparably to women in a perspective taking task after administration of intranasal oxytocin but not after placebo.

Directory of Open Access Journals (Sweden)

Angeliki eTheodoridou

2013-05-01

Full Text Available Oxytocin (OT is thought to play an important role in human interpersonal information processing and behavior. By inference, OT should facilitate empathic responding, i.e. the ability to feel for others and to take their perspective. In two independent double-blind, placebo-controlled between-subjects studies, we assessed the effect of intranasally administered OT on affective empathy and perspective taking, whilst also examining potential sex differences (e.g., women being more empathic than men. In study 1, we provided 96 participants (48 men with an empathy scenario and recorded self reports of empathic reactions to the scenario, while in study 2, a sample of 120 individuals (60 men performed a computerized implicit perspective taking task. Whilst results from Study 1 showed no influence of OT on affective empathy, we found in Study 2 that OT exerted an effect on perspective taking ability in men. More specifically, men responded faster than women in the placebo group but they responded as slowly as women in the OT group. We conjecture that men in the OT group adopted a social perspective taking strategy, such as did women in both groups, but not men in the placebo group. On the basis of results across both studies, we suggest that self-report measures (such as used in Study 1 might be less sensitive to OT effects than more implicit measures of empathy such as that used in Study 2. If these assumptions are confirmed, one could infer that OT effects on empathic responses are more pronounced in men than women, and that any such effect is best studied using more implicit measures of empathy rather than explicit self-report measures.

Feasibility study of a randomized controlled trial of a telephone-delivered problem-solving-occupational therapy intervention to reduce participation restrictions in rural breast cancer survivors undergoing chemotherapy.

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Hegel, Mark T; Lyons, Kathleen D; Hull, Jay G; Kaufman, Peter; Urquhart, Laura; Li, Zhongze; Ahles, Tim A

2011-10-01

Breast cancer patients receiving adjuvant chemotherapy often experience functional effects of treatment that limit participation in life activities. The purpose of this study was to examine the feasibility of conducting a randomized controlled trial (RCT) of a novel intervention for these restrictions, determine acceptability of the intervention, and preliminarily assess its effects. A pilot RCT of a telephone-delivered Problem-solving and Occupational Therapy intervention (PST-OT) to improve participation restrictions in rural breast cancer patients undergoing chemotherapy. Thirty-one participants with Stages 1-3 breast cancer were randomized to 6 weekly sessions of PST-OT (n = 15) and usual care (n = 16). The primary study outcome was the feasibility of conducting the trial. Secondary outcomes were functional, quality of life and emotional status as assessed at baseline, 6 and 12 weeks. Of 46 patients referred 31 were enrolled (67% recruitment rate), of which 6 participants withdrew (81% retention rate). Twenty-four participants completed all study-related assessments (77%). Ninety-two percent of PST-OT participants were highly satisfied with the intervention, and 92% reported PST-OT to be helpful/very helpful for overcoming participation restrictions. Ninety-seven percent of planned PST-OT treatment sessions were completed. Completion rates for PST-OT homework tasks were high. Measures of functioning, quality of life, and emotional state favored the PST-OT condition. This pilot study suggests that an RCT of the PST-OT intervention is feasible to conduct with rural breast cancer patients undergoing adjuvant chemotherapy and that PST-OT may have positive effects on function, quality of life, and emotional state. 2010 John Wiley & Sons, Ltd.

Clinical application of ocular trauma score for mechanical ocular injury in armed forces

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Ying ZHANG

2011-10-01

Full Text Available Objective To evaluate the predictive value of ocular trauma score(OTS in mechanical ocular injuries occurring in military forces,and compare the difference of OTS application between ocular injury caused by military training and non-military training.Methods The data of 633 inpatients from armed forces suffering from injury of 665 eyes were retrospectively reviewed.Of the 665 injured eyes,326 were injured during military training and 339 during non-military training.The likelihood of final visual acuity(VA calculated with the OTS score was compared with that by the present OTS study,and the correlation between the score and final VA was then analyzed.Results Compared with the distribution of final VA in standard OTS score,the ratio in category 1 was statistically different in present study(P 0.05.There were significant differences in ratio of category 3 between eyes injured by military and non-military training(P < 0.05,and both ratios in category 1 were significantly different compared with those in standard OTS(P < 0.01,e.g.the ratio of NLP was lower(50% vs 73%,P < 0.01;43% vs 73%,P < 0.01;and the ratio in category 2 was significantly different between the eyes injured by non-military training and standard OTS(P < 0.05.However,the OTS showed positive correlation with final VA in present study(P < 0.0001.Conclusion OTS calculated at initial examination may primarily provide a piece of prognostic information of mechanical ocular injuries,and is of special benefit for deciding a prompt management or for evacuation decision for severe ocular injuries caused by military training.

Sex differences in the neural and behavioral response to intranasal oxytocin and vasopressin during human social interaction.

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Rilling, James K; Demarco, Ashley C; Hackett, Patrick D; Chen, Xu; Gautam, Pritam; Stair, Sabrina; Haroon, Ebrahim; Thompson, Richmond; Ditzen, Beate; Patel, Rajan; Pagnoni, Giuseppe

2014-01-01

Both oxytocin (OT) and vasopressin (AVP) are known to modulate social behavior, and dysfunction in both systems has been postulated as a potential cause of certain psychiatric disorders that involve social behavioral deficits. In particular, there is growing interest in intranasal OT as a potential treatment for certain psychiatric disorders, and preliminary pre-clinical and clinical studies suggest efficacy in alleviating some of the associated symptoms. However, the vast majority of research participants in these studies have been male, and there is evidence for sexually differentiated effects of nonapeptides in both humans and non-human animals. To date, no study has investigated the effect of intranasal OT on brain function in human males and females within the same paradigm. Previously, in a randomized, placebo-controlled, double-blind fMRI study, we reported effects of intranasal OT and AVP on behavior and brain activity of human males as they played an interactive social game known as the Prisoner's Dilemma Game. Here, we present findings from an identical study in human females, and compare these with our findings from males. Overall, we find that both behavioral and neural responses to intranasal OT and AVP are highly sexually differentiated. In women, AVP increased conciliatory behavior, and both OT and AVP caused women to treat computer partners more like humans. In men, AVP increased reciprocation of cooperation from both human and computer partners. However, no specific drug effects on behavior were shared between men and women. During cooperative interactions, both OT and AVP increased brain activity in men within areas rich in OT and AVP receptors and in areas playing a key role in reward, social bonding, arousal and memory (e.g., the striatum, basal forebrain, insula, amygdala and hippocampus), whereas OT and AVP either had no effect or in some cases actually decreased brain activity in these regions in women. OT treatment rendered neural responses

Functional characterization of the NhaA Na+/H+ antiporter from the green picoalga Ostreococcus tauri.

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Dawut, Keatisuda; Sirisattha, Sophon; Hibino, Takashi; Kageyama, Hakuto; Waditee-Sirisattha, Rungaroon

2018-07-01

Transmembrane ion transport is a critical process in the cellular response to salt stress. Among the known functional membrane transporters that are involved in the salt stress response, Na + /H + antiporters have been extensively studied. These ubiquitous membrane proteins are crucial for salt tolerance and are associated with the regulation of internal pH, cell volume, morphogenesis, and vesicular trafficking. Molecular and functional analyses of Na + /H + antiporters have been characterized among taxa but little is known about algal Na + /H + antiporters. Here, we analyzed putative Na + /H + antiporters from the complete genome sequence of the marine picoalga Ostreococcus tauri. At least 10 putative Na + /H + antiporters belonging to the SOS1, NHX, and KEA/Kef families were found. Surprisingly, a bacterial type NhaA sequence (OtNhaA) was also found. Topological modeling of OtNhaA predicted 12 possible transmembrane segments with a long N-terminus. The full-length (FL_OtNhaA) and N-terminal truncated (ΔN112_OtNhaA) versions of OtNhaA were constructed, expressed in the salt-sensitive mutant Escherichia coli TO114, and functionally characterized. Complementation analysis revealed that FL_OtNhaA- and ΔN112_OtNhaA-expressing cells exhibited increased tolerance to high NaCl concentrations up to 700 mM. Antiporter activity assays showed that both FL_OtNhaA and ΔN112_OtNhaA proteins predominantly exhibited Na + /H + and Ca 2+ /H + antiporter activities at alkaline pH conditions. Intriguingly, the ΔN112_OtNhaA exhibited higher Na + /H + and Ca 2+ /H + antiporter activities compared to FL_OtNhaA. Kinetic analysis revealed that FL_OtNhaA has a high affinity for Na + and Ca 2+ ions with a K m of 1.1 ± 0.23 mM for Na + (at pH 8.5) and a K m of 0.3 ± 0.07 mM for Ca 2+ (at pH 8.5). Since NhaA has shown striking diversity among taxa, our results provide insight into the functional properties of the algal NhaA Na + /H + antiporter. These results will

NUTRIMENTAL POTENTIAL OF RED DOLICHOS, BROWN DOLICHOS AND COWPEA FOR GREEN MANURE PRODUCED UNDER THREE TILLAGE SYSTEMS

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Felix Alfredo Beltran-Morales

2009-06-01

Full Text Available The nutrimental content of N, NO3-N, P, K, Mg, Ca, Mn, Zn, Cu, Fe, and B, was evaluated in three bean cultivars of two species with potential to be used as green manure. Cultivars were the red lab-lab bean (RL (Lablab purpureus, brown lab-lab bean (BL (L. purpureus and cowpea (CW (Vigna unguiculata. Three tillage systems were assayed: conventional tillage (CT, minimum tillage (MT and optimal tillage (OT. Results showed that the nutrimental content of N, NO3, P, K did not evidence statistical differences (P≤0.05 among bean cultivars and tillage systems. However, the greater content of N was in BL-CT with 4.85%. The content of P varied from 0.38 to 0.41% and the concentration of K from 2.40 to 2.84%. Ca showed the highest concentration in RL-OT with 2.88%, while the lowest percentage was evidenced by CW-CT, with 2.12% (P≤0.05. The highest percentage of Mg appeared in CW-OT with 0.89% and the lowest concentration was registered for BL-CT, showing 0.52% (P≤0.05. The highest Fe concentration was observed in RL-OT with 0.15% and the lowest in BL-CT and RL-CT, with 0.10%. The Mn content was significantly greater in CW without concerning the tillage system with 0.39% and the smallest concentration was obtained in RL-MT with 0.17%. The greatest percentage content of nutriment B was observed in BL-OT, RL-CT, CW-MT and CW-OT, from 0.024 to 0.030%. In contrast, the lowest content of B was found in RL-MT with 0.015%. The content of Zn did not showed statistical differences respect to the cropping system; the lowest concentration of Zn was observed in CW-OT with 0.0026% and the highest percentage in BL-OT with 0.0040. Cu was significantly highest in BL-CT, BL-MT, BL-OT, RL-CT, RL-MT, RL-OT and CW-OT, being BL-MT and BL-OT the combinations with the highest concentration (0.0061%, while the lowest concentration was observed in CW-MT and CW-CT, with 0.0048% and 0.0044%, respectively.

Cyclic ADP-ribose and heat regulate oxytocin release via CD38 and TRPM2 in the hypothalamus during social or psychological stress in mice

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Jing Zhong

2016-07-01

Full Text Available Hypothalamic oxytocin (OT is released into the brain by cyclic ADP-ribose (cADPR with or without depolarizing stimulation. Previously, we showed that the intracellular free calcium concentration ([Ca2+]i that seems to trigger OT release can be elevated by -NAD+, cADPR, and ADP in mouse oxytocinergic neurons. As these -NAD+ metabolites activate warm-sensitive TRPM2 cation channels, when the incubation temperature is increased, the [Ca2+]i in hypothalamic neurons is elevated. However, it has not been determined whether OT release is facilitated by heat in vitro or hyperthermia in vivo in combination with cADPR. Furthermore, it has not been examined whether CD38 and TRPM2 exert their functions on OT release during stress or stress-induced hyperthermia in relation to the anxiolytic roles and social behaviors of OT under stress conditions. Here, we report that OT release from the isolated hypothalami of male mice in culture was enhanced by extracellular application of cADPR or increasing the incubation temperature from 35°C to 38.5°C, and simultaneous stimulation showed a greater effect. This release was inhibited by a cADPR-dependent ryanodine receptor inhibitor and a nonspecific TRPM2 inhibitor. The facilitated release by heat and cADPR was suppressed in the hypothalamus isolated from CD38 knockout mice and CD38- or TRPM2-knockdown mice. In the course of these experiments, we noted that OT release differed markedly between individual mice under stress with group housing. That is, when male mice received cage-switch stress and eliminated due to their social subclass, significantly higher levels of OT release were found in subordinates compared with ordinates. In mice exposed to anxiety stress in an open field, the cerebrospinal fluid (CSF OT level increased transiently at 5 minutes after exposure, and the rectal temperature also increased from 36.6°C to 37.8°C. OT levels in the CSF of mice with lipopolysaccharide-induced fever (+0.8�

Anatomy in Occupational Therapy Program Curriculum: Practitioners' Perspectives

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Schofield, Katherine Anne

2014-01-01

Anatomy education is undergoing significant transformation. It is unknown whether changes are in accordance with occupational therapy (OT) practice needs. The purpose of this pilot study was to survey OT clinicians to determine their perspectives on the value of anatomy in OT curricula, and anatomical knowledge required for practice. In addition…

Intranasal Oxytocin Normalizes Amygdala Functional Connectivity in Posttraumatic Stress Disorder.

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Koch, Saskia B J; van Zuiden, Mirjam; Nawijn, Laura; Frijling, Jessie L; Veltman, Dick J; Olff, Miranda

2016-07-01

The neuropeptide oxytocin (OT) has been suggested as a promising pharmacological agent for medication-enhanced psychotherapy in posttraumatic stress disorder (PTSD) because of its anxiolytic and prosocial properties. We therefore investigated the behavioral and neurobiological effects of a single intranasal OT administration (40 IU) in PTSD patients. We conducted a randomized, placebo-controlled, cross-over resting-state fMRI study in male and female police officers with (n=37, 21 males) and without PTSD (n=40, 20 males). We investigated OT administration effects on subjective anxiety and functional connectivity of basolateral (BLA) and centromedial (CeM) amygdala subregions with prefrontal and salience processing areas. In PTSD patients, OT administration resulted in decreased subjective anxiety and nervousness. Under placebo, male PTSD patients showed diminished right CeM to left ventromedial prefrontal cortex (vmPFC) connectivity compared with male trauma-exposed controls, which was reinstated after OT administration. Additionally, female PTSD patients showed enhanced right BLA to bilateral dorsal anterior cingulate cortex (dACC) connectivity compared with female trauma-exposed controls, which was dampened after OT administration. Although caution is warranted, our findings tentatively suggest that OT has the potential to diminish anxiety and fear expression of the amygdala in PTSD, either via increased control of the vmPFC over the CeM (males) or via decreased salience processing of the dACC and BLA (females). Our findings add to accumulating evidence that OT administration could potentially enhance treatment response in PTSD.

Non-linear learning in online tutorial to enhance students’ knowledge on normal distribution application topic

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Kartono; Suryadi, D.; Herman, T.

2018-01-01

This study aimed to analyze the enhancement of non-linear learning (NLL) in the online tutorial (OT) content to students’ knowledge of normal distribution application (KONDA). KONDA is a competence expected to be achieved after students studied the topic of normal distribution application in the course named Education Statistics. The analysis was performed by quasi-experiment study design. The subject of the study was divided into an experimental class that was given OT content in NLL model and a control class which was given OT content in conventional learning (CL) model. Data used in this study were the results of online objective tests to measure students’ statistical prior knowledge (SPK) and students’ pre- and post-test of KONDA. The statistical analysis test of a gain score of KONDA of students who had low and moderate SPK’s scores showed students’ KONDA who learn OT content with NLL model was better than students’ KONDA who learn OT content with CL model. Meanwhile, for students who had high SPK’s scores, the gain score of students who learn OT content with NLL model had relatively similar with the gain score of students who learn OT content with CL model. Based on those findings it could be concluded that the NLL model applied to OT content could enhance KONDA of students in low and moderate SPK’s levels. Extra and more challenging didactical situation was needed for students in high SPK’s level to achieve the significant gain score.

Preparation of open tubular capillary columns by in situ ring-opening polymerization and their applications in cLC-MS/MS analysis of tryptic digest.

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Wang, Hongwei; Yao, Yating; Li, Ya; Ma, Shujuan; Peng, Xiaojun; Ou, Junjie; Ye, Mingliang

2017-08-01

An open tubular (OT) column (25 μm i.d.) was prepared by in situ ring-opening polymerization of octaglycidyldimethylsilyl polyhedral oligomeric silsesquioxanes (POSS-epoxy) with 4-aminophenyl disulfide (APDS) in a binary porogenic system of ethanol/H 2 O. It was found that porogenic composition played an important role in the formation of OT stationary phases. The ratio of ethanol/H 2 O at 6/1 (v/v) would lead to the fabrication of hybrid monoliths, while the ratio of ethanol/H 2 O at 13/1 (v/v) would result in the synthesis of OT phases. In addition, the effects of precursor content and reaction duration on the thickness of OT stationary phases were investigated. Either lower precursor content or shorter reaction duration would produce thinner layer of OT column. The repeatability of OT columns was evaluated through relative standard deviation (RSD%) with benzene as the analyte. The run-to-run, column-to-column and batch-to-batch repeatabilities were 1.7%, 4.8% and 5.6%, respectively, exhibiting satisfactory repeatability of the OT column. Then tryptic digest of mouse liver proteins was used to evaluate the performance of the resulting OT columns (25 μm i.d. × 2.5 m in length) by cLC-MS/MS analysis, demonstrating their potential in proteome analysis. Copyright © 2017 Elsevier B.V. All rights reserved.

Differential Axonal Projection of Mitral and Tufted Cells in the Mouse Main Olfactory System

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Shin Nagayama

2010-09-01

Full Text Available In the past decade, much has been elucidated regarding the functional organization of the axonal connection of olfactory sensory neurons to olfactory bulb (OB glomeruli. However, the manner in which projection neurons of the OB process odorant input and send this information to higher brain centers remains unclear. Here, we report long-range, large-scale tracing of the axonal projection patterns of OB neurons using two-photon microscopy. Tracer injection into a single glomerulus demonstrated widely distributed mitral/tufted cell axonal projections on the lateroventral surface of the mouse brain, including the anterior/posterior piriform cortex (PC and olfactory tubercle (OT. We noted two distinct groups of labeled axons: PC-orienting axons and OT-orienting axons. Each group occupied distinct parts of the lateral olfactory tract. PC-orienting axons projected axon collaterals to a wide area of the PC but only a few collaterals to the OT. OT-orienting axons densely projected axon collaterals primarily to the anterolateral OT (alOT. Different colored dye injections into the superficial and deep portions of the OB external plexiform layer revealed that the PC-orienting axon populations originated in presumed mitral cells and the OT-orienting axons in presumed tufted cells. These data suggest that although mitral and tufted cells receive similar odor signals from a shared glomerulus, they process the odor information in different ways and send their output to different higher brain centers via the PC and alOT.

OpenTopography: Addressing Big Data Challenges Using Cloud Computing, HPC, and Data Analytics

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Crosby, C. J.; Nandigam, V.; Phan, M.; Youn, C.; Baru, C.; Arrowsmith, R.

2014-12-01

OpenTopography (OT) is a geoinformatics-based data facility initiated in 2009 for democratizing access to high-resolution topographic data, derived products, and tools. Hosted at the San Diego Supercomputer Center (SDSC), OT utilizes cyberinfrastructure, including large-scale data management, high-performance computing, and service-oriented architectures to provide efficient Web based access to large, high-resolution topographic datasets. OT collocates data with processing tools to enable users to quickly access custom data and derived products for their application. OT's ongoing R&D efforts aim to solve emerging technical challenges associated with exponential growth in data, higher order data products, as well as user base. Optimization of data management strategies can be informed by a comprehensive set of OT user access metrics that allows us to better understand usage patterns with respect to the data. By analyzing the spatiotemporal access patterns within the datasets, we can map areas of the data archive that are highly active (hot) versus the ones that are rarely accessed (cold). This enables us to architect a tiered storage environment consisting of high performance disk storage (SSD) for the hot areas and less expensive slower disk for the cold ones, thereby optimizing price to performance. From a compute perspective, OT is looking at cloud based solutions such as the Microsoft Azure platform to handle sudden increases in load. An OT virtual machine image in Microsoft's VM Depot can be invoked and deployed quickly in response to increased system demand. OT has also integrated SDSC HPC systems like the Gordon supercomputer into our infrastructure tier to enable compute intensive workloads like parallel computation of hydrologic routing on high resolution topography. This capability also allows OT to scale to HPC resources during high loads to meet user demand and provide more efficient processing. With a growing user base and maturing scientific user

How does maternal oxytocin influence children's mental health problem and maternal mental health problem?

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Tse, Wai S; Siu, Angela F Y; Wong, Tracy K Y

2017-12-01

This study aims to explore the interrelationship among maternal oxytocin (OT) responsiveness, maternal mental health, maternal parenting behavior, and mental health of children under a free-play interaction. 61 mother-child dyads were recruited for the study. Maternal mental health problem and parenting self-efficacy were measured using self-reported questionnaires. The mental health problems of children were also evaluated using a mother-reported questionnaire. Furthermore, salivary OT was collected before and after a standardized 10min free-play interaction. Parenting behaviors, including eye gaze and touch, were measured during the free-play interaction. Maternal OT responsiveness was significantly associated with less maternal mental health problem, touch frequency, and mental health problem of children but not with parenting self-efficacy. In the multivariate linear regression analysis that considers maternal OT responsiveness and maternal and children's mental health problems, maternal OT responsiveness was not associated with the mental health problems of children. This result suggested that maternal mental health problem played a mediational role between maternal OT responsiveness and the mental health problem of children. Results supported the assertion that maternal OT responsiveness contributed to the increased risk of maternal mental health problems and, subsequently, the risk of mental health problems of their children. Copyright © 2017 Elsevier B.V. All rights reserved.

Oxytocin promotes social bonding in dogs.

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Romero, Teresa; Nagasawa, Miho; Mogi, Kazutaka; Hasegawa, Toshikazu; Kikusui, Takefumi

2014-06-24

Recent evidence suggests that enduring social bonds have fitness benefits. However, very little is known about the neural circuitry and neurochemistry underlying the formation and maintenance of stable social bonds outside reproductive contexts. Oxytocin (OT), a neuropeptide synthetized by the hypothalamus in mammals, regulates many complex forms of social behavior and cognition in both human and nonhuman animals. Animal research, however, has concentrated on monogamous mammals, and it remains unknown whether OT also modulates social bonds in nonreproductive contexts. In this study we provide behavioral evidence that exogenous OT promotes positive social behaviors in the domestic dog toward not only conspecifics but also human partners. Specifically, when sprayed with OT, dogs showed higher social orientation and affiliation toward their owners and higher affiliation and approach behaviors toward dog partners than when sprayed with placebo. Additionally, the exchange of socio-positive behaviors with dog partners triggered the release of endogenous OT, highlighting the involvement of OT in the development of social relationships in the domestic dog. These data provide new insight into the mechanisms that facilitate the maintenance of close social bonds beyond immediate reproductive interest or genetic ties and complement a growing body of evidence that identifies OT as one of the neurochemical foundations of sociality in mammalian species.

Facial Feature Extraction Using Frequency Map Series in PCNN

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Rencan Nie

2016-01-01

Full Text Available Pulse coupled neural network (PCNN has been widely used in image processing. The 3D binary map series (BMS generated by PCNN effectively describes image feature information such as edges and regional distribution, so BMS can be treated as the basis of extracting 1D oscillation time series (OTS for an image. However, the traditional methods using BMS did not consider the correlation of the binary sequence in BMS and the space structure for every map. By further processing for BMS, a novel facial feature extraction method is proposed. Firstly, consider the correlation among maps in BMS; a method is put forward to transform BMS into frequency map series (FMS, and the method lessens the influence of noncontinuous feature regions in binary images on OTS-BMS. Then, by computing the 2D entropy for every map in FMS, the 3D FMS is transformed into 1D OTS (OTS-FMS, which has good geometry invariance for the facial image, and contains the space structure information of the image. Finally, by analyzing the OTS-FMS, the standard Euclidean distance is used to measure the distances for OTS-FMS. Experimental results verify the effectiveness of OTS-FMS in facial recognition, and it shows better recognition performance than other feature extraction methods.

Oxytocin and vasopressin enhance responsiveness to infant stimuli in adult marmosets.

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Taylor, Jack H; French, Jeffrey A

2015-09-01

The neuropeptides oxytocin (OT) and arginine-vasopressin (AVP) have been implicated in modulating sex-specific responses to offspring in a variety of uniparental and biparental rodent species. Despite the large body of research in rodents, the effects of these hormones in biparental primates are less understood. Marmoset monkeys (Callithrix jacchus) belong to a clade of primates with a high incidence of biparental care and also synthesize a structurally distinct variant of OT (proline instead of leucine at the 8th amino acid position; Pro(8)-OT). We examined the roles of the OT and AVP systems in the control of responses to infant stimuli in marmoset monkeys. We administered neuropeptide receptor agonists and antagonists to male and female marmosets, and then exposed them to visual and auditory infant-related and control stimuli. Intranasal Pro(8)-OT decreased latencies to respond to infant stimuli in males, and intranasal AVP decreased latencies to respond to infant stimuli in females. Our study is the first to demonstrate that Pro(8)-OT and AVP alter responsiveness to infant stimuli in a biparental New World monkey. Across species, the effects of OT and AVP on parental behavior appear to vary by species-typical caregiving responsibilities in males and females. Copyright © 2015 Elsevier Inc. All rights reserved.

Impact of International Collaborative Project on Cultural Competence among Occupational Therapy Students

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Divya Sood OTD, OTR/L

2014-07-01

Full Text Available Occupational therapy (OT educators recognize a need to ensure that OT students are culturally competent. The researchers developed the International Collaborative Project on Cultural Competence (ICPCC to help students understand the impact of cultural context on client care. Entry-level MOT students from a university in the US (N = 18 collaborated with BOT students (N = 4 and advanced MOT students (N = 9 from two universities in India using an online course management system WebCT. The study explored the impact of the ICPCC on OT students’ cultural competence and discusses students’ perceptions of culture on the OT process. The Inventory for Assessing the Process of Cultural Competence Among Health Care Professionals Revised© measured students’ cultural competence at baseline and immediately after participation in the ICPCC. Qualitative data was collected using a Self-Reflection Form. There was an increase in the cultural competence scores among all three groups of students after participating in the ICPCC at p value < .05. Three themes emerged from the qualitative data analysis: meaning of the term culture, impact of cultural on client- centered practice, and impact of cultural on OT outcomes. OT students recognized the role that cultural differences play in OT evaluation and intervention.

Low dose intranasal oxytocin delivered with Breath Powered device dampens amygdala response to emotional stimuli: A peripheral effect-controlled within-subjects randomized dose-response fMRI trial.

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Quintana, Daniel S; Westlye, Lars T; Alnæs, Dag; Rustan, Øyvind G; Kaufmann, Tobias; Smerud, Knut T; Mahmoud, Ramy A; Djupesland, Per G; Andreassen, Ole A

2016-07-01

It is unclear if and how exogenous oxytocin (OT) reaches the brain to improve social behavior and cognition and what is the optimal dose for OT response. To better understand the delivery routes of intranasal OT administration to the brain and the dose-response, we compared amygdala response to facial stimuli by means of functional magnetic resonance imaging (fMRI) in four treatment conditions, including two different doses of intranasal OT using a novel Breath Powered device, intravenous (IV) OT, which provided similar concentrations of blood plasma OT, and placebo. We adopted a randomized, double-blind, double-dummy, crossover design, with 16 healthy male adults administering a single-dose of these four treatments. We observed a treatment effect on right amygdala activation during the processing of angry and happy face stimuli, with pairwise comparisons revealing reduced activation after the 8IU low dose intranasal treatment compared to placebo. These data suggest the dampening of amygdala activity in response to emotional stimuli occurs via direct intranasal delivery pathways rather than across the blood-brain barrier via systemically circulating OT. This trial is registered at the U.S. National Institutes of Health clinical trial registry (www.clinicaltrials.gov; NCT01983514) and as EudraCT no. 2013-001608-12. Copyright © 2016 Elsevier Ltd. All rights reserved.

Oxytocin effects on emotional response to others' faces via serotonin system in autism: A pilot study.

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Fukai, Mina; Hirosawa, Tetsu; Kikuchi, Mitsuru; Ouchi, Yasuomi; Takahashi, Tetsuya; Yoshimura, Yuko; Miyagishi, Yoshiaki; Kosaka, Hirotaka; Yokokura, Masamichi; Yoshikawa, Etsuji; Bunai, Tomoyasu; Minabe, Yoshio

2017-09-30

The oxytocin (OT)-related serotonergic system is thought to play an important role in the etiology and social symptoms of autism spectrum disorder (ASD). However, no evidence exists for the relation between the prosocial effect of chronic OT administration and the brain serotonergic system. Ten male subjects with ASD were administered OT for 8-10 weeks in an open-label, single-arm, non-randomized, uncontrolled manner. Before and during the OT treatment, positron emission tomography was used with the ( 11 C)-3-amino-4-(2-[(demethylamino)methyl]phenylthio)benzonitrile( 11 C-DASB) radiotracer. Then binding of serotonin transporter ( 11 C-DASB BP ND ) was estimated. The main outcome measures were changes in 11 C-DASB BP ND and changes in the emotional response to others' faces. No significant change was found in the emotional response to others' faces after the 8-10 week OT treatment. However, the increased serotonin transporter (SERT) level in the striatum after treatment was correlated significantly with increased negative emotional response to human faces. This study revealed a relation between changes in the serotonergic system and in prosociality after chronic OT administration. Additional studies must be conducted to verify the chronic OT effects on social behavior via the serotonergic system. Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.

Crystallization and preliminary X-ray crystallographic studies of the biotin carboxyl carrier protein and biotin protein ligase complex from Pyrococcus horikoshii OT3

International Nuclear Information System (INIS)

Bagautdinov, Bagautdin; Matsuura, Yoshinori; Bagautdinova, Svetlana; Kunishima, Naoki

2007-01-01

A truncated form of biotin carboxyl carrier protein containing the C-terminal half fragment (BCCPΔN76) and the biotin protein ligase (BPL) with the mutation R48A (BPL*) or the double mutation R48A K111A (BPL**) were successfully cocrystallized in the presence of ATP and biotin. The BPL*–BCCPΔN76 and BPL**–BCCPΔN76 crystals belong to space group P2 1 and diffract X-rays to 2.7 and 2.0 Å resolution, respectively. Biotin protein ligase (BPL) catalyses the biotinylation of the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase. To elucidate the exact details of the protein–protein interactions in the biotinylation function, the C-terminal half fragment of BCCP (BCCPΔN76), the R48A mutant of BPL (BPL*) and the R48A K111A double mutant of BPL (BPL**), all of which are from Pyrococcus horikoshii OT3, have been expressed, purified and successfully cocrystallized. Cocrystals of the BPL*–BCCPΔN76 and BPL**–BCCPΔN76 complexes as well as crystals of BPL*, BPL** and BCCPΔN76 were obtained by the oil-microbatch method using PEG 20 000 as a precipitant at 295 K. Complete X-ray diffraction data sets for BPL*–BCCPΔN76 and BPL**–BCCPΔN76 crystals were collected at 100 K to 2.7 and 2.0 Å resolution, respectively, using synchrotron radiation. They belong to the monoclinic space group P2 1 , with similar unit-cell parameters a = 69.85, b = 63.12, c = 75.64 Å, β = 95.9°. Assuming two subunits of the complex per asymmetric unit gives a V M value of 2.45 Å 3 Da −1 and a solvent content of 50%

Clinical features and course of ocular toxocariasis in adults.

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Seong Joon Ahn

2014-06-01

Full Text Available PURPOSE: To investigate the clinical features, clinical course of granuloma, serologic findings, treatment outcome, and probable infection sources in adult patients with ocular toxocariasis (OT. METHODS: In this retrospective cohort study, we examined 101 adult patients diagnosed clinically and serologically with OT. Serial fundus photographs and spectral domain optical coherence tomography images of all the patients were reviewed. A clinic-based case-control study on pet ownership, occupation, and raw meat ingestion history was performed to investigate the possible infection sources. RESULTS: Among the patients diagnosed clinically and serologically with OT, 69.6% showed elevated immunoglobulin E (IgE levels. Granuloma in OT involved all retinal layers and several vitreoretinal comorbidities were noted depending on the location of granuloma: posterior pole granuloma was associated with epiretinal membrane and retinal nerve fiber layer defects, whereas peripheral granuloma was associated with vitreous opacity. Intraocular migration of granuloma was observed in 15 of 93 patients (16.1%. Treatment with albendazole (400 mg twice a day for 2 weeks and corticosteroids (oral prednisolone; 0.5-1 mg/kg/day resulted in comparable outcomes to patients on corticosteroid monotherapy; however, the 6-month recurrence rate in patients treated with combined therapy (17.4% was significantly lower than that in patients treated with corticosteroid monotherapy (54.5%, P=0.045. Ingestion of raw cow liver (80.8% or meat (71.2% was significantly more common in OT patients than healthy controls. CONCLUSIONS: Our study discusses the diagnosis, treatment, and prevention strategies for OT. Evaluation of total IgE, in addition to anti-toxocara antibody, can assist in the serologic diagnosis of OT. Combined albendazole and corticosteroid therapy may reduce intraocular inflammation and recurrence. Migrating feature of granuloma is clinically important and may further suggest

Role of the oxytocin system in amygdala subregions in the regulation of social interest in male and female rats.

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Dumais, Kelly M; Alonso, Andrea G; Bredewold, Remco; Veenema, Alexa H

2016-08-25

We previously found that oxytocin (OT) receptor (OTR) binding density in the medial amygdala (MeA) correlated positively with social interest (i.e., the motivation to investigate a conspecific) in male rats, while OTR binding density in the central amygdala (CeA) correlated negatively with social interest in female rats. Here, we determined the causal involvement of OTR in the MeA and CeA in the sex-specific regulation of social interest in adult rats by injecting an OTR antagonist (5ng/0.5μl/side) or OT (100pg/0.5μl/side) before the social interest test (4-min same-sex juvenile exposure). OTR blockade in the CeA decreased social interest in males but not females, while all other treatments had no behavioral effect. To further explore the sex-specific involvement of the OT system in the CeA in social interest, we used in vivo microdialysis to determine possible sex differences in endogenous OT release in the CeA during social interest. Interestingly, males and females showed similar levels of extracellular OT release at baseline and during social interest, suggesting that factors other than local OT release mediate the sex-specific role of CeA-OTR in social interest. Moreover, we found a positive correlation between CeA-OT release and social investigation time in females. This was further reflected by reduced CeA-OT release during social interest in females that expressed low compared to high social interest. We discuss the possibility that this reduction in OT release may be a consequence, rather than a cause, of exposure to a social stimulus. Overall, our findings show for the first time that extracellular OT release in the CeA is similar between males and females and that OTR in the CeA plays a causal role in the regulation of social interest toward juvenile conspecifics in males. Copyright © 2016 IBRO. Published by Elsevier Ltd. All rights reserved.

Enkephalin inhibition of angiotensin-stimulated release of oxytocin and vasopressin

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Keil, L. C.; Chee, O.; Rosella-Dampman, L. M.; Emmert, S.; Summy-Long, J. Y.

1984-01-01

The effect of intracerebroventricular (ICV) pretreatment with 100 ng/5 microliter leucine(5)-enkephalin (LE) on the increase in plasma oxytocin (OT) and vasopressin (VP) caused by ICV injection of 10, 50, or 100 ng/5 microliter of angiotensin II (AII) is investigated experimentally in conscious adult male Sprague-Dawley rats; the effects of water-deprivation dehydration and lactation/suckling (in female rats) are also studied. An OT radioimmunoassay (RIA) with a sensitivity of 800 fg/ml (described in detail) and the VP RIA technique of Keil and Severs (1977) are employed. Administration of AII or dehydration for 48 or 72 h cause a significant increase in OT and VP without affecting the ratio, while lactation and suckling increase OT only. LE pretreatment inhibits significantly but does not suppress the AII-stimulated OT-VP response.

Short outlines of books by Estonian authors / Brita Melts, Rutt Hinrikus and Janika Kronberg

Index Scriptorium Estoniae

Melts, Brita

2013-01-01

Arvustus: Baturin, Nikolai. Lendav hollandlanna. Tallinn : Eesti Raamat, 2012 ; Friedenthal, Meelis. Mesilased. Tallinn : Varrak, 2012 ; Kaus, Jan. Koju. Tallinn : Tuum, 2012 ; Kaldmaa, Kätlin. Armastuse tähestik. Tallinn : NyNorden, 2012 ; Kivastik, Mart. Vietnami retsept. Tartu : Väike Öömuusika, 2012 ; Õnnepalu, Tõnu. Kuidas on elada. Tallinn : Varrak, 2012 ; Õnnepalu, Tõnu. Mandala. Tallinn : Varrak, 2012 ; Riismaa, Kaur. Rebase matmine. Tallinn : Näo Kirik, 2012 ; Vadi, Urmas. Tagasi Eestisse. Saarde-Pärnu : Jumalikud Ilmutused, 2012 ; Vint, Toomas. Avalikult abielust. Tallinn : Tulikiri, 2012 ; Kangro, Maarja. Dantelik auk. Tallinn : Eesti Keele Sihtasutus, 2012 ; Kaevats, Mihkel. Ungari kirsid & teisi luuletusi. Tallinn : Pegasus, 2012.

Milliseid erialaseid teadmisi ja oskusi olete kasutanud oma praeguses ametis? : vastavad infoteaduse eriala vilistlased / interv. Hela Ojasaar, Margot Mahlapuu

Index Scriptorium Estoniae

2005-01-01

Vastavad: EV Presidendi Kantselei direktori asetäitja, üldosakonna juhataja Riina Roomet, Mahtra Talurahvamuuseumi direktor Tiia-Helle Schmitte, EBS-i raamatukogu juhataja Eda Pihu, Euroopa Liidu asjade komisjoni nõunik (Riigikogu) Külli Kask, Kultuuriministeeriumi raamatukogunõunik Meeli Veskus, Võrumaa Keskraamatukogu direktor Inga Kuljus, Lääne Maakonna Keskraamatukogu direktor Ilme Sepp, Riigiarhiivi direktor Marge Tiidus, kirjastuse Pilgrim juht Tiina Ristimets, Tallinna Keskraamatukogu direktor Kaie Holm, Eesti ENIC/NARIC Keskuse infospetsialist Liia Tüür, Eesti Rahvusraamatukogu direktor Tiiu Valm, Tartu Ülikooli Raamatukogu teenindusdirektor Mare-Nelli Ilus, Viljandi Kultuuriakadeemia raamatukogunduse ja infoteaduste osakonna juhataja Ilmar Vaaro

Vasopressin and oxytocin receptor systems in the brain: Sex differences and sex-specific regulation of social behavior.

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Dumais, Kelly M; Veenema, Alexa H

2016-01-01

The neuropeptides vasopressin (VP) and oxytocin (OT) and their receptors in the brain are involved in the regulation of various social behaviors and have emerged as drug targets for the treatment of social dysfunction in several sex-biased neuropsychiatric disorders. Sex differences in the VP and OT systems may therefore be implicated in sex-specific regulation of healthy as well as impaired social behaviors. We begin this review by highlighting the sex differences, or lack of sex differences, in VP and OT synthesis in the brain. We then discuss the evidence showing the presence or absence of sex differences in VP and OT receptors in rodents and humans, as well as showing new data of sexually dimorphic V1a receptor binding in the rat brain. Importantly, we find that there is lack of comprehensive analysis of sex differences in these systems in common laboratory species, and we find that, when sex differences are present, they are highly brain region- and species-specific. Interestingly, VP system parameters (VP and V1aR) are typically higher in males, while sex differences in the OT system are not always in the same direction, often showing higher OT expression in females, but higher OT receptor expression in males. Furthermore, VP and OT receptor systems show distinct and largely non-overlapping expression in the rodent brain, which may cause these receptors to have either complementary or opposing functional roles in the sex-specific regulation of social behavior. Though still in need of further research, we close by discussing how manipulations of the VP and OT systems have given important insights into the involvement of these neuropeptide systems in the sex-specific regulation of social behavior in rodents and humans. Copyright © 2015 Elsevier Inc. All rights reserved.

Early involvement in friendships predicts later plasma concentrations of oxytocin and vasopressin in juvenile rhesus macaques (Macaca mulatta

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Tamara Aliza Rachel Weinstein

2014-08-01

Full Text Available The neuropeptides oxytocin (OT and vasopressin (AVP are involved in social bonding in attachment relationships, but their role in friendship is poorly understood. We investigated whether rhesus macaques’ (Macaca mulatta friendships at age one predicted plasma OT and AVP at two later time points. Subjects were 54 rhesus macaques at the California National Primate Research Center. Blood was drawn during a brief capture-and-release in the home cage, and plasma assayed for OT and AVP using an enzyme immunoassay. Separate linear mixed models for each sex tested the effects of dominance rank, age, sampling time point, housing condition, parturition status, two blood draw timing measures, and five friendship types: proximity friendships, play friendships, reciprocal friendships (a preference for a peer that also preferred the subject, multiplex friendships (friendships displayed in more than one behavioral domain, and total number of friendships. Females’ number of reciprocal and play friendships at age one significantly predicted later OT; additionally, these two friendship types interacted with rank, such that high-ranking females with the fewest friendships had the highest OT concentrations. Friendship did not predict later OT levels in males, however proximity, play, reciprocal, and total number of friendships predicted males’ plasma AVP. Play and total number of friendships also tended to predict AVP in females. Our results show that peripheral measures of neuroendocrine functioning in juvenile rhesus monkeys are influenced by early involvement in friendships. Friendships have an especially strong impact on an individual’s psychosocial development, and our data suggest OT and AVP as potential underlying mechanisms. Moreover, sex differences in the functioning of the OT and AVP systems, and their relation to friendship, may have important clinical implications for the use of OT as a therapeutic, as well as informing the social context in

o-Toluidine blood protein adducts: HPLC analysis with fluorescence detection after a single dose in the adult male rat

International Nuclear Information System (INIS)

Cheever, K.L.; DeBord, G.D.; Swearengin, T.F.

1991-01-01

Hemoglobin (Hb) and albumin (Alb) adducts of the suspect human carcinogen o-toluidine (OT) were quantified in blood samples collected from rats after a single i.p. injection. Mild alkaline hydrolysis of Hb-adducted [ 14 C]OT followed by extraction with ethylacetate resulted in recovery of 66% of the bound radioactivity. HPLC analysis revealed a single radiolabeled peak which was identified as OT by GC-MS. In subsequent experiments the Hb and Alb adduct levels were determined by HPLC analysis of this split product using fluorescence detection. 4-Ethylaniline was used as internal standard. The detection limit for OT was approximately 450 pg/injection of 5 pmol. mg Hb. Mean adduct levels for Hb increased rapidly over the first 4 hr with the highest (ng/mg Hb ± SD) 3.7 ± 0.5 detected 24 hr after OT (50 mg/kg body wt). In contrast, adduct levels for pooled Alb samples increased from 0.7 ng/mg Alb at 2 hr to 2.5 ng/mg Alb at 4 hr, but were not detectable 24 hr after OT (50 mg/kg body wt). In contrast, adduct levels for pooled Alb samples increased from 0.7 ng/mg Alb at 2 hr to 2.5 ng/mg Alb at 4 hr, but were not detectable 24 hr after dosing. Hb adducts showed a linear relationship for OT doses of 10, 20, 40, 50, and 100 mg/kg body wt. The Hb adduct t 1/2 (11.2 days) was determined after a single 100 mg/kg OT dose. Hb adduct levels were quantifiable (1.3 ± 0.2 ng/mg Hb) by HPLC/fluorescence 28 days after 100 mg/kg OT

Vasopressin and oxytocin receptor systems in the brain: sex differences and sex-specific regulation of social behavior

Science.gov (United States)

Dumais, Kelly M.; Veenema, Alexa H.

2015-01-01

The neuropeptides vasopressin (VP) and oxytocin (OT) and their receptors in the brain are involved in the regulation of various social behaviors and have emerged as drug targets for the treatment of social dysfunction in several sex-biased neuropsychiatric disorders. Sex differences in the VP and OT systems may therefore be implicated in sex-specific regulation of healthy as well as impaired social behaviors. We begin this review by highlighting the sex differences, or lack of sex differences, in VP and OT synthesis in the brain. We then discuss the evidence showing the presence or absence of sex differences in VP and OT receptors in rodents and humans, as well as showing new data of sexually dimorphic V1a receptor binding in the rat brain. Importantly, we find that there is lack of comprehensive analysis of sex differences in these systems in common laboratory species, and we find that, when sex differences are present, they are highly brain region- and species- specific. Interestingly, VP system parameters (VP and V1aR) are typically higher in males, while sex differences in the OT system are not always in the same direction, often showing higher OT expression in females, but higher OT receptor expression in males. Furthermore, VP and OT receptor systems show distinct and largely non-overlapping expression in the rodent brain, which may cause these receptors to have either complementary or opposing functional roles in the sex-specific regulation of social behavior. Though still in need of further research, we close by discussing how manipulations of the VP and OT systems have given important insights into the involvement of these neuropeptide systems in the sex-specific regulation of social behavior in rodents and humans. PMID:25951955

Maternal and Paternal Plasma, Salivary, and Urinary Oxytocin and Parent-Infant Synchrony: Considering Stress and Affiliation Components of Human Bonding

Science.gov (United States)

Feldman, Ruth; Gordon, Ilanit; Zagoory-Sharon, Orna

2011-01-01

Studies in mammals have implicated the neuropeptide oxytocin (OT) in processes of bond formation and stress modulation, yet the involvement of OT in human bonding throughout life remains poorly understood. We assessed OT in the plasma, saliva, and urine of 112 mothers and fathers interacting with their 4-6-month-old infants. Parent-infant…

Clinical relevance of novel Otarine herpesvirus-3 in California sea lions (Zalophus californianus: lymphoma, esophageal ulcers, and strandings

Directory of Open Access Journals (Sweden)

Venn-Watson Stephanie

2012-12-01

Full Text Available Abstract Herpesviruses have been recognized in marine mammals, but their clinical relevance is not always easy to assess. A novel otarine herpesvirus-3 (OtHV3 was detected in a geriatric California sea lion (Zalophus californianus, and using a newly developed quantitative PCR assay paired with histology, OtHV3 was associated with esophageal ulcers and B cell lymphoblastic lymphoma in this animal. The prevalence and quantities of OtHV3 were then determined among buffy coats from 87 stranded and managed collection sea lions. Stranded sea lions had a higher prevalence of OtHV3 compared to managed collection sea lions (34.9% versus 12.5%; p = 0.04, and among the stranded sea lions, yearlings were most likely to be positive. Future epidemiological studies comparing the presence and viral loads of OtHV3 among a larger population of California sea lions with and without lymphoid neoplasia or esophageal ulcers would help elucidate the relevance of OtHV3-associated pathologies to these groups.

The effect of oxytocin on cooperation in a prisoner’s dilemma depends on the social context and a person’s social value orientation

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Boone, Christophe; Kiyonari, Toko

2014-01-01

The interactionist approach to the study of exogenous oxytocin (OT) effects on prosocial behavior has emphasized the need to consider both contextual cues and individual differences. Therefore, an experiment was set up to examine the joint effect of intranasal OT, a salient social cue and the personality trait social value orientation on cooperative behavior in one-shot prisoner’s dilemma games. The outcome of these mixed-motive games is known to be highly dependent on values and on social information that might reveal the partner’s intent. Consistent with an a priori hypothesis, OT and social information interact significantly to affect the behavior of individuals with a proself value orientation: after prior contact with the game partner, OT enhances cooperative behavior, whereas in anonymous conditions, it exacerbates their intrinsic self-interested behavior. These effects of OT do not hold for individuals with a prosocial value orientation, whose cooperation levels appear to be more influenced by prior contact with the game partner. Follow-up hypotheses for why prosocial and proself individuals respond differently to exogenous OT were developed. PMID:23588271

Autoradiographic demonstration of oxytocin-binding sites in the macula densa

International Nuclear Information System (INIS)

Stoeckel, M.E.; Freund-Mercier, M.J.

1989-01-01

Specific oxytocin (OT)-binding sites were localized in the rat kidney with use of a selective 125 I-labeled OT antagonist ( 125 I-OTA). High concentrations of OT binding sites were detected on the juxtaglomerular apparatus with use of the conventional film autoradiographic technique. No labeling occurred on other renal structures. The cellular localization of the OT binding sites within the juxtaglomerular apparatus was studied in light microscope autoradiography, on semithin sections from paraformaldehyde-fixed kidney slices incubated in the presence of 125 I-OTA. These preparations revealed selective labeling of the macula densa, mainly concentrated at the basal pole of the cells. Control experiments showed first that 125 I-OTA binding characteristics were not noticeably altered by prior paraformaldehyde fixation of the kidneys and second that autoradiographic detection of the binding sites was not impaired by histological treatments following binding procedures. In view of the role of the macula densa in the tubuloglomerular feedback, the putative OT receptors of this structure might mediate the stimulatory effect of OT on glomerular filtration