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Sample records for k60 polysaccharides extracted

  1. Extraction Optimization of Polysaccharides from Pitaya Stems

    Institute of Scientific and Technical Information of China (English)

    HE Cong-fen; LI Peng; ZHAO Hua; SONG Li-ya; ZHU Jun; DONG Yin-mao

    2011-01-01

    [Objective] The aim was to describe the extraction of polysaccharides from pitaya stems.[Method] The hot water,enzyme-assisted and microwave-assisted methods were used,with the microwave-assisted extraction being deemed optimal by general evaluation.[Result] The main factors affecting the yield of polysaccharides in the microwave-assisted extraction,by order of magnitude,were as follows:timemicrowave powertemperature;additionally,optimal conditions included a 10 min extraction time,an 80 ℃ extraction temperature and a microwave setting of 200 W.Using these optimal conditions,the yield of PSPS(Polysaccharides from Pitaya Stems) was 1.42%.After purification,the yield of PSPS was 0.74%.[Conclusion] The PSPS was analyzed by IR,MALDI-TOF-MS and an element analysis technique.It was shown to be a polysaccharide mixture,and the molecular weight was between 3 900 and 4 300 Da.

  2. [The extraction, purification and assaying of Gynostemma pentaphyllum polysaccharides].

    Science.gov (United States)

    Song, Shu-liang; Tang, Jin-bao; Ji, Ai-guo; Liang, Hao; Zhu, Peng; Wang, Wei-li

    2006-06-01

    By orthogonal design, and considering extracting efficiency and cost, optimizing the extract method of Gynostemma pentaphyllum polysaccharides. We purified the crude Gynostemma pentaphyllum polysaccharides initially, and assayed the polysaccharides content of Gynostemma pentaphyllum polysccharides. The content of Gynostemma pentaphyllum polysaccharides was sigificantly higher than the predecessor. It would provide conditions for the deep exploitation of Gynostemma pentaphyllum.

  3. α-Amylase-assisted extraction of polysaccharides from Panax ginseng.

    Science.gov (United States)

    Sun, Lin; Wu, Di; Ning, Xin; Yang, Guang; Lin, Ziheng; Tian, Meihong; Zhou, Yifa

    2015-04-01

    In this paper, α-amylase-assisted extraction was used to isolate the polysaccharide that remained in hot water-extracted ginseng. The yield of the polysaccharide was 9.0%, almost equal to that of the hot water-extracted polysaccharide. Using anion exchange and gel permeation chromatography, the polysaccharide was fractionated into a neutral polysaccharide fraction and six pectic fractions. The neutral fraction accounted for 76% of the polysaccharide and contained both amylopectin and amylose. The pectic polysaccharide fractions were identified to be arabinogalactan, type-I rhamnogalacturonan and homogalacturonan-type pectin by high-performance liquid chromatography, Fourier transform-infrared and nuclear magnetic resonance analysis. Structural and lymphocyte proliferation activity results showed that these polysaccharides were different from those extracted by hot water, indicating that ginseng contains complex polysaccharides with diverse structures, which results in its diverse pharmacological activities. The α-amylase-assisted extraction is a novel method for preparing ginseng polysaccharides and could be applied toward the further study and exploration of ginseng. These findings provide technical and theoretical support for ginseng pharmacology. Copyright © 2015 Elsevier B.V. All rights reserved.

  4. Separation of Pneumcoccal Capsular Polysaccharide BY Gel Extraction

    Institute of Scientific and Technical Information of China (English)

    Xu Xiaoping; Huang Xinghua; Li Zhongqin; Chen Jiebo

    2004-01-01

    Separation of pneumcoccal capsular polysaccharide by gel was investigated in this paper. The gels used here were poly(acrylamide-co-sodium methacrylate)gels and prepared by free radical polymerization in aqueous solution. The experimental results indicated that gel extraction is a potential method to separate pneumcoccal capsular polysaccharide from its dilute solution.

  5. Optimization of enzyme assisted extraction of polysaccharides from Ganoderma lucidum

    Institute of Scientific and Technical Information of China (English)

    Xu Chunhua; Tian Zhenle; Zhang Chenju; Yu Xiaobing; Zheng Huihua

    2014-01-01

    In the present work,an enzyme assisted extraction method is used to isolate crude polysaccharides from Ganoderma lucidum. The isolating effect was optimized with orthographic graph statistic method with three levels and four independent variables. Complex enzyme,extraction temperature,extraction time and ex-traction pH were combined to obtain the best possible combination to get maximum amount of extract and crude polysaccharides yield. The optimum extraction conditions were:complex enzyme amount of 3%(w/v),extrac-tion temperature at 45℃,extraction time of 3 h and extraction pH at 7. Under these conditions,the experimen-tal amount of extract is 8.9%and the yield of crude polysaccharides is 1.1%,which are in close agreement with the value predicted by the model.

  6. Microwave-assisted extraction of polysaccharides from solanum nigrum

    Institute of Scientific and Technical Information of China (English)

    CHEN Xiao-qing; LIU-qin; JIANG xin-yu; ZENG fan

    2005-01-01

    The microwave-assisted method was used to extract polysaccharides from solanum nigrum. The optimum experimental parameters, mechanism of the extraction and the effect of microwave-assisted extraction process on the structures of polysaccharides were investigated. The extract was analyzed by the modified phenol-sulfuric acid method at 490 nm. The optimum experimental parameters were obtained by orthogonal experiments as follows: extraction time 15 min, microwave radiation power 455 W and the process ratio of materials mass to solvent volume 1∶20. The results show that compared with the conventional reflux extraction, the microwave-assisted extraction has a higher yield in shorter time, with no effect on the finally obtained polysaccharides as seen from the FT-IR spectra. The scanning electron microscopy images reveal that the mechanism of the extraction is related to the structural changes of the plant cells in different extracting conditions.

  7. Extraction and antioxidant activity of polysaccharides from Rana chensinensis skin.

    Science.gov (United States)

    Wang, Zhanyong; Zhao, Yuanyuan; Su, Tingting

    2015-01-22

    The extraction process of polysaccharides from Rana chensinensis skin was optimized by using a Box-Behnken design. The optimum extraction conditions were as follows: extraction time, 4.96h; extraction temperature, 100°C; ratio of water to raw material, 60; and extraction frequency, 1. Under these conditions, the experimental polysaccharide yield was 2.03±0.14%, which agreed with the predicted yield. The purified polysaccharide RCSP II was successfully obtained by diethylaminoethanol-Sepharose and Sepharose CL-6B column chromatography. In vitro experiments showed that RCSP II exhibited a strong scavenging activity against superoxide anion and 1,1-diphenyl-2-picrylhydrazyl radicals but a weak scavenging activity against hydroxyl radicals. RCSP II also showed a strong reducing capacity. Thus, this polysaccharide can be used as a natural antioxidant in functional foods or medicines.

  8. Ultrasound assisted extraction of polysaccharides from hazelnut skin.

    Science.gov (United States)

    Yılmaz, Tuncay; Tavman, Şebnem

    2016-03-01

    In this study ultrasound assisted extraction (UAE) of polysaccharides from hazelnut skin has been studied. Optimum sonication time has been evaluated depending on responses such as amount of carbohydrate and dried sample and thermogravimetric analysis. Chemical and structural properties of extracted material have been determined by Fourier transform spectroscopy attenuated-total reflectance (FTIR-ATR) spectroscopy. Pretreated hazelnut skin powders were extracted in distilled water. Mixture was sonicated by ultrasonic processor probe for 15, 30, 45, 60, 90, and 120 min. The results of UAE showed that maximum ethanol insoluble extracts in 60 min and the highest dry matter content could be obtained in 120 min extraction. Although total carbohydrate content of ethanol insoluble dry extract decreased with time, total carbohydrate in ethanol soluble fraction increased. Polysaccharides extracted from hazelnut skin were assumed to be pectic polysaccharide according to the literature survey of FTIR analysis result. Application time of UAE has an important effect on extraction of polysaccharide from hazelnut skin. This affect could be summarized by enhancing extraction yield up to critical level. Decrease of the yield in ethanol insoluble part could be explained by polymer decomposition. Most suitable model was hyperbolic model by having the lowest root mean square error and the highest R(2) values.

  9. Extraction of Opuntia dillenii Haw. Polysaccharides and Their Antioxidant Activities

    OpenAIRE

    Heng Li; Qingxia Yuan; Xianjiao Zhou; Fuhua Zeng; Xiangyang Lu

    2016-01-01

    Use of natural polysaccharides in medicine and food has wide interest in research. In this study, we extracted and purified some polysaccharides from cactus Opuntia dillenii Haw. (ODP). Some preliminary functions of these products were characterized. Under the optimal purification conditions, the yield of ODP extracted from the 2–4 month-old Opuntia dillenii Haw. (T-ODP) was 30.60% ± 0.40%, higher than that of ODP from the 5–10 month-old materials (O-ODP) (18.97% ± 0.58%). The extracted ODP w...

  10. Polysaccharide extraction from Sphallerocarpus gracilis roots by response surface methodology.

    Science.gov (United States)

    Ma, Tingting; Sun, Xiangyu; Tian, Chengrui; Luo, Jiyang; Zheng, Cuiping; Zhan, Jicheng

    2016-07-01

    The extraction process of Sphallerocarpus gracilis root polysaccharides (SGRP) was optimized using response surface methodology with two methods [hot-water extraction (HWE) and ultrasonic-assisted extraction (UAE)]. The antioxidant activities of SGRP were determined, and the structural features of the untreated materials (HWE residue and UAE residue) and the extracted polysaccharides were compared by scanning electron microscopy. Results showed that the optimal UAE conditions were extraction temperature of 81°C, extraction time of 1.7h, liquid-solid ratio of 17ml/g, ultrasonic power of 300W and three extraction cycles. The optimal HWE conditions were 93°C extraction temperature, 3.6h extraction time, 21ml/g liquid-solid ratio and three extraction cycles. UAE offered a higher extraction yield with a shorter time, lower temperature and a lower solvent consumption compared with HWE, and the extracted polysaccharides possessed a higher antioxidant capacity. Therefore, UAE could be used as an alternative to conventional HWE for SGRP extraction.

  11. Extraction, Characterization and Immunological Activity of Polysaccharides from Rhizoma gastrodiae

    Directory of Open Access Journals (Sweden)

    Juncheng Chen

    2016-06-01

    Full Text Available A response surface and Box-Behnken design approach was applied to augment polysaccharide extraction from the residue of Rhizoma gastrodiae. Statistical analysis revealed that the linear and quadratic terms for three variables during extraction exhibited obvious effects on extraction yield. The optimum conditions were determined to be a liquid-to-solid ratio of 54 mL/g, an extraction temperature of 74 °C, an extraction time of 66 min, and three extractions. These conditions resulted in a maximum Rhizoma gastrodiae polysaccharide (RGP extraction yield of 6.11% ± 0.13%. Two homogeneous polysaccharides (RGP-1a and RGP-1b were obtained using DEAE cellulose-52 and Sephadex G-100 columns. The preliminary characterization of RGP-1a and RGP-1b was performed using HPLC-RID, HPGPC, and FTIR. Tests of the immunological activity in vitro showed that the two polysaccharides could significantly stimulate macrophages to release NO and enhance phagocytosis in a dose-dependent manner. In particular, RGP-1b (200 μg/mL and LPS (2 μg/mL had almost the same influence on the NO production and phagocytic activity of RAW 264.7 macrophages (p > 0.05. All the data obtained indicate that RGP-1a and RGP-1b have the potential to be developed as a health food.

  12. Extraction, Characterization and Immunological Activity of Polysaccharides from Rhizoma gastrodiae.

    Science.gov (United States)

    Chen, Juncheng; Tian, Shan; Shu, Xiaoying; Du, Hongtao; Li, Na; Wang, Junru

    2016-06-25

    A response surface and Box-Behnken design approach was applied to augment polysaccharide extraction from the residue of Rhizoma gastrodiae. Statistical analysis revealed that the linear and quadratic terms for three variables during extraction exhibited obvious effects on extraction yield. The optimum conditions were determined to be a liquid-to-solid ratio of 54 mL/g, an extraction temperature of 74 °C, an extraction time of 66 min, and three extractions. These conditions resulted in a maximum Rhizoma gastrodiae polysaccharide (RGP) extraction yield of 6.11% ± 0.13%. Two homogeneous polysaccharides (RGP-1a and RGP-1b) were obtained using DEAE cellulose-52 and Sephadex G-100 columns. The preliminary characterization of RGP-1a and RGP-1b was performed using HPLC-RID, HPGPC, and FTIR. Tests of the immunological activity in vitro showed that the two polysaccharides could significantly stimulate macrophages to release NO and enhance phagocytosis in a dose-dependent manner. In particular, RGP-1b (200 μg/mL) and LPS (2 μg/mL) had almost the same influence on the NO production and phagocytic activity of RAW 264.7 macrophages (p > 0.05). All the data obtained indicate that RGP-1a and RGP-1b have the potential to be developed as a health food.

  13. The Effect of Different Extracting Agents on Extraction Rate of Polysaccharides in Shii- Takes from Guizhou

    Institute of Scientific and Technical Information of China (English)

    MO; Liping

    2001-01-01

    Herba Glossogynes Tenuifoliae (Shii-Take) contains lots of bioactive components, such as equal amylose, mixed amylose, sugar albumen, albumen polysaccharide, proflin and so on, that all have effects of anticancer and restrain tumour. But the extraction rate by hot water and alkali solution is much low.  Different Shii-Takes contain polysaccharides with different properties and structures, such as acid-base property, solubility, stability and so on. So the property of extracting agent is one of important effects on extraction rate, in addition cell structures of Shii-Takes. Extraction of polysaccharides from four species of Herba Glossogynes Tenuifoliae (Shii-Take) from Guizhou in different extracting agents is studied in this paper. Extraction conditions are 0.5mol/L aqueous solution of extracting agents, 40 times of weight ratio of liquid and solid during 1hour at 60℃. The quantities of the polysaccharides extracted shows the following Table 1.……

  14. The Effect of Different Extracting Agents on Extraction Rate of Polysaccharides in Shii- Takes from Guizhou

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@ Herba Glossogynes Tenuifoliae (Shii-Take) contains lots of bioactive components, such as equal amylose, mixed amylose, sugar albumen, albumen polysaccharide, proflin and so on, that all have effects of anticancer and restrain tumour. But the extraction rate by hot water and alkali solution is much low. Different Shii-Takes contain polysaccharides with different properties and structures, such as acid-base property, solubility, stability and so on. So the property of extracting agent is one of important effects on extraction rate, in addition cell structures of Shii-Takes. Extraction of polysaccharides from four species of Herba Glossogynes Tenuifoliae (Shii-Take) from Guizhou in different extracting agents is studied in this paper. Extraction conditions are 0.5mol/L aqueous solution of extracting agents, 40 times of weight ratio of liquid and solid during 1hour at 60℃. The quantities of the polysaccharides extracted shows the following Table 1.

  15. Effect of Extraction Methods on Polysaccharide of Clitocybe maxima Stipe

    OpenAIRE

    Junchen Chen; Pufu Lai; Hengsheng Shen; Hengguang Zhen; Rutao Fang

    2013-01-01

    Clitocybe maxima (Gartn. ex Mey. Fr.) Quél. is a favorable edible fungi species. The proportion of its stipe is about 45% of entire fruit biomass, which is a low value defined byproduct. To increase its value added utilization, three extraction methods (as hot water, microwave-assisted and complex-enzyme-hydrolysis-assist) were conducted. The extraction effect on the polysaccharide of Clitocybe maxima stipe was compared and the processing conditions in extraction were optimized. The content o...

  16. Microwave superheated water extraction of polysaccharides from spent coffee grounds.

    Science.gov (United States)

    Passos, Cláudia P; Coimbra, Manuel A

    2013-04-15

    The spent coffee grounds (SCG) are a food industry by-product that can be used as a rich source of polysaccharides. In the present work, the feasibility of microwave superheated water extraction of polysaccharides from SCG was studied. Different ratios of mass of SCG to water, from 1:30 to 1:5 (g:mL) were used for a total volume of 80 mL. Although the amount of material extracted/batch (MAE1) increased with the increase of the concentration of the sample, the amount of polysaccharides achieved a maximum of 0.57 g/batch for 1:10. Glycosidic-linkage composition showed that all extraction conditions allowed to obtain mainly arabinogalactans. When the unextracted insoluble material was re-extracted under the same conditions (MAE2), a further extraction of polysaccharides was observed (0.34 g/batch for 1:10), mainly galactomannans. Also, a high amount of oligosaccharides, mainly derived from galactomannans, can be obtained in MAE2 (0.96 g/batch for 1:10). This technology allows to obtain galactomannans and arabinogalactans in proportions that are dependent on the operating conditions.

  17. Extraction, purification and antioxidant activity of polysaccharides from bamboo leaves

    Institute of Scientific and Technical Information of China (English)

    GAO Ya-ni; TIAN Cheng-rui; ZHAO Li-li

    2012-01-01

    Ultrasonic extraction (UE) was employed for the extraction of bamboo leaf polysaccharides (BLP).The influential parameters of UE procedure including extraction time,ultrasonic power and solid/liquid ratio were optimized by orthogonal experiments.DEAE-cellulose column chromatography was applied to purify BLP and then the radical scavenging activity of BLP was also evaluated.Optimal extraction conditions were:extraction time of 15 min,ultrasonic power of 300 W,and solid/liquid ratio of 1:15.Four kinds of polysaccharides were obtained by DEAE-cellulose column chromatography; the maximum superoxide radical scavenging rate (20.4%) of BLP was inferior to that of vitamin C (Vc,the control) and the hydroxyl radical scavenging rate (50%) was equivalent to that of Vc.

  18. Extraction and purification of the polysaccharides in Hippohaere rhamnoides L.

    Institute of Scientific and Technical Information of China (English)

    XU Yaqin; JIN Ting; YU Zeyuan; FU Hong

    2007-01-01

    The different extraction technology and purification technology of Hippohpae rhamoides polysaccharides were researched in the paper. The best method of papain extraction were obtained, the ratio of papain 2%, pH at 5.5, temperature at 45℃ and extraction time of 20 min were suitable for papain extraction. The highest content of Hippohpae rhamoides polysaccharides was 44.28 mg·g-1. The optimum process of ultrasonic extraction were obtained, namely extracted for 55 min at 480 W with the material ratio of 1:20. The highest content of Hippohpae rhamoides polysaccharides was 48.63 mg·g-1. The results showed that the ultrasonic and papain extraction together was the best method, the content was 54.30 mg·g-1. After the removing protein, pigment and dialysis. Two fraction were separated from the purified Hippohpae rhamoides by DEAE-cellulose chromatography, the main fraction was collected finally. The fraction was identified by Sepharose CL-4B gel filtration. Ultraviolet spectrometry, freeze-thawing analysis showed that fraction was purified. Its molecular weight was probably 109.4 ku.

  19. Extraction, purification and characterization of polysaccharides from Hawk tea.

    Science.gov (United States)

    Jia, Xuejing; Ding, Chunbang; Yuan, Shu; Zhang, Zhongwei; Chen, Yang'er; Du, Lei; Yuan, Ming

    2014-01-01

    In the present study, the extraction, purification and characterization of polysaccharides from Hawk mature leaf tea (HMP) were investigated. The optimal extraction parameters were obtained by using a Box-Behnken design as follows: extraction temperature 88.9 °C, extraction time 128.2 min and ratio of water to solid 11.4 mL/g. The crude HMP was sequentially purified by chromatography of DEAE-52, and two purified fractions, HMP-1 and HMP-2, were obtained. HMP-1 and HMP-2 were mainly composed of arabinose, galactose, glucose and mannose with the molecular weight of 133 and 100 kDa, respectively. For antioxidant activities in vitro, HMP-1 had strong 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity and ferric reducing activity power (FRAP). These results provide a scientific basis for the further use of polysaccharides from this traditional herb tea. Copyright © 2013 Elsevier Ltd. All rights reserved.

  20. Polysaccharides in Sipunculus nudus: Extraction condition optimization and antioxidant activities

    Science.gov (United States)

    Zhang, Qin; Dong, Lanfang; Tong, Tong; Wang, Qingchao; Xu, Mingzhu

    2017-02-01

    Marine organisms constitute unlimited resource of bioactive substances due to their high biodiversity and represent a valuable source of new compounds. This study optimized the alkali-extraction conditions and antioxidant activities of soluble polysaccharides from the body wall of Sipunculus nudus. The effects of solid-liquid ratio, extraction duration, extraction temperature, and alkali concentration on the yield of S. nudus polysaccharides (SNP) were examined, according to which the optimal combination of extraction parameters was obtained by an orthogonal test. The relative influencing importance of different extraction parameters on the yield of SNP followed the order as solid-liquid ratio > extraction temperature > alkali concentration > extraction duration. The highest extraction yield, 1.98%, was achieved under an optimal extraction condition: temperature 60°C, solid-liquid ratio 1:6 g mL-1, duration 5 h, and alkali (NaOH) mass fraction 6%. The in vitro antioxidant activities examination showed that extracted SNP under this optimized condition had strong power in reducing certain hydroxyl and superoxide radical scavenging abilities. The promising results showed that extracted SNP could be a potent natural antioxidant.

  1. Extraction of Opuntia dillenii Haw. Polysaccharides and Their Antioxidant Activities.

    Science.gov (United States)

    Li, Heng; Yuan, Qingxia; Zhou, Xianjiao; Zeng, Fuhua; Lu, Xiangyang

    2016-11-24

    Use of natural polysaccharides in medicine and food has wide interest in research. In this study, we extracted and purified some polysaccharides from cactus Opuntia dillenii Haw. (ODP). Some preliminary functions of these products were characterized. Under the optimal purification conditions, the yield of ODP extracted from the 2-4 month-old Opuntia dillenii Haw. (T-ODP) was 30.60% ± 0.40%, higher than that of ODP from the 5-10 month-old materials (O-ODP) (18.97% ± 0.58%). The extracted ODP was purified by DEAE sepharose fast flow anion exchange and Sephacryl S-400 chromatography with four fractions obtained (ODP-Ia, ODP-Ib, ODP-IIa and ODP-IIb). Analysis with UV-vis chromatography indicated that ODP-Ia and ODP-IIa were relatively homogeneous molecules with a molecular weight of 339 kD and 943 kD, respectively. Results of infrared spectroscopy indicated that ODP, ODP-Ia, and ODP-IIa were acidic polysaccharides. Further, the antioxidant activity against DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, hydroxyl radicals, and superoxide radical in vitro demonstrated that the T-ODP exhibited higher antioxidant activity than the O-ODP, and the purified fraction (ODP-Ia) was superior to the ODP. These results will offer a theoretical basis for further research on the structure-function relationship of ODP and the rational utilization of Opuntia dillenii Haw.

  2. Influence of Temperature on the Extractibility of Polysaccharides in Barley

    Directory of Open Access Journals (Sweden)

    Rodica Căpriţă

    2011-10-01

    Full Text Available Barley contains substantial amounts of both soluble and insoluble non-starch polysaccharides (NSP. The main watersoluble NSP in barley are highly viscous β-glucans. Monogastric animals, including humans and birds, cannotsynthesize β-glucanase, and the amount of β-glucanase derived from barley grain and bacteria in the gastrointestinaltract is insufficient to completely hydrolyze β-glucans. In the present investigation, we have studied the influence oftemperature and heating time on the extractibility of soluble polysaccharides in barley. Heating the barley samples at60°C and 80°C before extraction has the effect of lowering the soluble fraction of the polysaccharides. The dynamicviscosity values of water extracts from barley decreased up to 21.68% when heating at 60ºC for 15 minutes, and upto 25.30% when heating at 80ºC for 15 minutes, when the determinations were made immediately after extractseparation. Heating the barley samples for 15 minutes at 80°C deactivates the endogenous hydrolytic enzymes.

  3. Extraction of Opuntia dillenii Haw. Polysaccharides and Their Antioxidant Activities

    Directory of Open Access Journals (Sweden)

    Heng Li

    2016-11-01

    Full Text Available Use of natural polysaccharides in medicine and food has wide interest in research. In this study, we extracted and purified some polysaccharides from cactus Opuntia dillenii Haw. (ODP. Some preliminary functions of these products were characterized. Under the optimal purification conditions, the yield of ODP extracted from the 2–4 month-old Opuntia dillenii Haw. (T-ODP was 30.60% ± 0.40%, higher than that of ODP from the 5–10 month-old materials (O-ODP (18.97% ± 0.58%. The extracted ODP was purified by DEAE sepharose fast flow anion exchange and Sephacryl S-400 chromatography with four fractions obtained (ODP-Ia, ODP-Ib, ODP-IIa and ODP-IIb. Analysis with UV-vis chromatography indicated that ODP-Ia and ODP-IIa were relatively homogeneous molecules with a molecular weight of 339 kD and 943 kD, respectively. Results of infrared spectroscopy indicated that ODP, ODP-Ia, and ODP-IIa were acidic polysaccharides. Further, the antioxidant activity against DPPH (1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radicals, and superoxide radical in vitro demonstrated that the T-ODP exhibited higher antioxidant activity than the O-ODP, and the purified fraction (ODP-Ia was superior to the ODP. These results will offer a theoretical basis for further research on the structure-function relationship of ODP and the rational utilization of Opuntia dillenii Haw.

  4. Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune

    NARCIS (Netherlands)

    Klaus, A.; Kozarski, M.; Niksic, M.; Jakovljevic, D.; Todorovic, N.; Griensven, van L.J.L.D.

    2011-01-01

    Antioxidant properties of hot water extract (HWE), hot water extracted polysaccharides (HWP) and hot alkali extracted polysaccharides (HWAE) were obtained from fruiting bodies of the wild basidiomycete Schizophyllum commune. All extracts contained both a- and ß-glucans as determined by Megazyme ß-gl

  5. Extraction, characterization and antioxidant activities of polysaccharides of Chuanminshen violaceum.

    Science.gov (United States)

    Dong, Hongmin; Zhang, Qing; Li, Yu; Li, Lu; Lan, Weijie; He, Jingliu; Li, Huiyan; Xiong, Yabo; Qin, Wen

    2016-05-01

    A single factor experiment and Box-Behnken design were applied to optimize the microwave-assisted extraction (MAE) of Chuanminshen violaceum polysaccharides (CVPs). The CVPs yield was 34.59 ± 0.51%, which was in accordance with the predicted value of 35.54%, under the following optimum conditions: microwave power of 466 W, extraction temperature of 64.5 °C, extraction time of 15 min, and water-raw material ratio of 40 mL/g. Two CVPs fractions (CVPs-I and CVPs-II) were obtained via stepwise ethanol precipitation. CVPs-II was further purified using DEAE cellulose-52 chromatography to obtain the major fraction of CVPs-IIa. Fourier transform infrared spectroscopy (FTIR) and GC-MS analysis revealed that crude CVPs, CVPs-I, and CVPs-II were neutral polysaccharides and were mainly composed of glucose and galactose. The molecular weights of CVPs-I and CVPs-IIa were 233.69 and 11.02 kDa, respectively. Furthermore, all samples exhibited certain antioxidant activity or reducing power to scavenge the DPPH and ABTS radicals, among which CVPs-II was the strongest. In conclusion, MAE is an efficient method for extracting CVPs of C. violaceum, and this plant root has the potential to be explored as a source of natural antioxidants.

  6. Ultrasound-Assisted Extraction May Not Be a Better Alternative Approach than Conventional Boiling for Extracting Polysaccharides from Herbal Medicines

    OpenAIRE

    Ka-Man Yip; Jun Xu; Wing-Sum Tong; Shan-Shan Zhou; Tao Yi; Zhong-Zhen Zhao; Hu-Biao Chen

    2016-01-01

    In clinical practice polysaccharides from herbal medicines are conventionally prepared by boiling water extraction (BWE), while ultrasound-assisted extraction (UAE) has often been used instead employed in laboratory research due to its strong extraction ability and efficiency. However, if and how the polysaccharides obtained by UAE and BWE are comparable, and hence whether the UAE-based research is instructive for the actual usage of herbal polysaccharides still requires further evaluation. T...

  7. Extraction, Structural Characterization, and Potential Antioxidant Activity of the Polysaccharides from Four Seaweeds

    OpenAIRE

    Jinzhe He; Yaoyang Xu; Hongbo Chen; Peilong Sun

    2016-01-01

    Four seaweed polysaccharides were extracted from Sarcodia ceylonensis, Ulva lactuca L., Gracilaria lemaneiformis, and Durvillaea antarctica, respectively, by microwave-assisted extraction. The effect of three significant variables (extraction time, extraction temperature, and the ratio of water to raw material) on the process for extracting polysaccharides was investigated, along with the optimization of the extraction using the response surface method (RSM) with a Box–Behnken design. The pol...

  8. Extraction, Characterization, and Molecular Weight Determination of Senna tora (L.) Seed Polysaccharide

    OpenAIRE

    Pawar, Harshal A.; K. G. Lalitha

    2015-01-01

    The objective of the present work was extraction of polysaccharide from Senna tora L. seed and its characterization as a pharmaceutical excipient. Polysaccharide extraction was based on mechanical separation of the endosperm of seeds of Senna tora, water dissolution, centrifugation, and precipitation with acetone. Standard procedures were used to study the viscosity, micromeritic properties, and microbial bioburden. Accelerated stability study was carried out on isolated polysaccharide for si...

  9. Optimization of Extraction Process for Polysaccharide in Salvia Miltiorrhiza Bunge Using Response Surface Methodology.

    Science.gov (United States)

    Yanhua, Wang; Fuhua, Wu; Zhaohan, Guo; Mingxing, Peng; Yanan, Zhang; Ling, Pang Zhen; Minhua, Du; Caiying, Zhang; Zian, Liang

    2015-01-01

    This study was aimed to optimize the extraction process for Salvia miltiorrhiza Bunge polysaccharide using response surface methodology The results showed that four operating parameters including microwave power, microwave time and the particle size had notable effects on the polysaccharide extraction of Salvia miltiorrhiza Bunge. The effects could be ranked in decreasing order of importance as follows:. Microwave power > microwave time > the comminution degree. The optimal extraction parameters were determined as 573.83W of Microwave power and 8.4min of microwave time and 67.51mesh of the comminution degree, resulting in the yield of Salvia miltiorrhiza Bunge polysaccharide of 101.161mg / g. The established regression model describing polysaccharide extraction from as a function of the three extraction parameters was highly significant (R 2 = 0.9953). The predicted and experimental results were found to be in good agreement. Thus, the model can be applicable for the prediction of polysaccharide extraction from Salvia miltiorrhiza Bunge.

  10. Extraction of polysaccharides and the antioxidant activity from the seeds of Plantago asiatica L.

    Science.gov (United States)

    Ye, Chun-Lin; Hu, Wei-Lian; Dai, De-Hui

    2011-11-01

    The extraction conditions of polysaccharides from Plantago asiatica L. seeds were investigated. Four parameters affecting the polysaccharides extraction, extraction times, water to sample, extraction temperature and single extraction time, were determined by orthogonal experiments. Under the optimized conditions, the polysaccharides yield of P. asiatica L. seeds was 2.467%. The antioxidant activities of the polysaccharides were investigated. The reducing power of the polysaccharides was dose dependent, and the reducing capacity of the polysaccharides was inferior to butylated hydroxytoluene, which is known to be a strong reducing agent. The scavenging rates of the polysaccharides on superoxide and 1,1-diphenyl-2-picrylhydrazyl radicals were 79.7% and 81.4%, at polysaccharides concentration of 0.75 mg/mL, respectively, a scavenging rates approximately similar to that of 0.75 mg/mL ascorbic acid (83.5% and 85.1%, respectively). Furthermore, it exhibited a moderate concentration-dependent ABTS radical scavenging activity, ferrous ion chelating potency and H(2)O(2) scavenging activity. The data obtained in the in vitro models clearly establish the antioxidant potency of the polysaccharides extracted from Semen Plantaginis.

  11. Optimization of ultrasonic circulating extraction of polysaccharides from Asparagus officinalis using response surface methodology.

    Science.gov (United States)

    Zhao, Qingsheng; Kennedy, John F; Wang, Xiaodong; Yuan, Xiaofan; Zhao, Bing; Peng, Youshun; Huang, Yunxiang

    2011-08-01

    Polysaccharides were extracted from Asparagus officinalis. A novel ultrasonic circulating extraction (UCE) technology was applied for the polysaccharide extraction. Three-factor-three-level Box-Behnken design was employed to optimize ultrasonic power, extraction time and the liquid-solid ratio to obtain a high polysaccharide yield. The optimal extraction conditions were as follows: ultrasonic power was 600 W, extraction time was 46 min, the liquid-solid ratio was 35 mL/g. Under these conditions, the experimental yield of polysaccharides was 3.134%, which was agreed closely to the predicted value. The average molecular weight of A. officinalis polysaccharide was about 6.18×10(4) Da. The polysaccharides were composed of glucose, fucose, arabinose, galactose and rhamnose in a ratio of 2.18:1.86:1.50:0.98:1.53. Compared with hot water extraction (HWE), UCE showed time-saving, higher yield and no influence on the structure of asparagus polysaccharides. The results indicated that ultrasonic circulating extraction technology could be an effective and advisable technique for the large scale production of plant polysaccharides. Copyright © 2011 Elsevier B.V. All rights reserved.

  12. Translational Medicine Study on Extraction and Determination of Urtica Polysaccharides and Their Anti-Oxidation Effect

    Institute of Scientific and Technical Information of China (English)

    JIANG Zhi-tao; LI Shan-shan; WANG Tong-fang; CHEN Xiao-feng

    2016-01-01

    Objective: Translational medicine study on extraction and determination of urtica polysaccharides and their anti-oxidation effect. Methods: Water-soluble alcohol sedimentation technique was used to extract the urtica polysaccharides, and ultraviolet (UV) spectrophotometry was applied to determine their content. The influence of polysaccharides on the activities of glutathione (GSH), catalase (CAT), malondialdéhyde (MDA) and superoxyde dismutase (SOD) in mice with H22-loaded tumors was observed. Results: The purity of urtica polysaccharides was 61.39% after extraction and puriifcation. After administration of high-, moderate- and low-dose urtica polysaccharides, the activity of serum GSH, CAT and SOD was markedly higher while MDA content was prominently lower in mice with H22-loaded tumors than those in model group. Conclusion: Urtica polysaccharides can strengthen the anti-oxidation effect and inhibit the lipid peroxidation reaction in body.

  13. Optimization of enzyme assisted extraction of polysaccharides from Astragalus membranaceus.

    Science.gov (United States)

    Chen, Huaguo; Zhou, Xin; Zhang, Junzeng

    2014-10-13

    Astragalus polysaccharide (APS) is known to have a variety of pharmacological activities. In the present study, enzyme assisted extraction of APS from Astragalus mongholicus using various enzymes were examined. Research found that glucose oxidase offered a better performance in enhancement of extraction yields of APS than other ones. Glucose oxidase assisted extraction process was further optimized by using response surface method (RSM) to obtain maximum yield of crude APS. The optimized extraction conditions were as follows: enzyme amount of 3.0%, enzyme treated time of 3.44 d, enzyme treated temperature of 56.9 °C and extraction solvent pH of 7.8. Under these conditions, the experimental yield was 29.96 ± 0.14%, which was well in close agreement with the value (30.19%) predicted by RSM model and increased more than 250% compared with none enzyme treated ones. Pharmacological test showed that enzyme assisted APS had a better antioxidant activity (about 2 times higher) than none enzyme treated ones.

  14. Screening of natural polysaccharides extracted from the fruits of Pithecellobium dulce as a pharmaceutical adjuvant.

    Science.gov (United States)

    S, Preethi; A, Mary Saral

    2016-11-01

    Polysaccharides were extracted from the dried fruiting bodies of Pithecellobium dulce with 20% ethanol by microwave-assisted extraction. The polysaccharides were isolated by ion exchange chromatography and afford three water-soluble polysaccharides PDP-1, PDP-2, and PDP-3. These isolated compounds were subjected to acid hydrolysis, methylation, IR and GC-MS for its compositional analysis and revealed that all the three fractions are heteropolysaccharides. PDP-1 was found to be composed of xylose, mannose, galactose and Rhamnose. PDP-2 and PDP-3 composed of xylose, Rhamnose, glucose, ribose, galactose, and mannose. The micromeretic properties of the extracted polysaccharides possessed a bulk density of 0.69g/ml, 0.65g/ml and 0.71g/ml for PDP-1, PDP-2, and PDP-3 respectively. The Hausner's ratio and Carr's index confirm the good flow property and compressibility of the polysaccharides. The polysaccharides extracted from Pithecellobium dulce fruits were tested for its application as a pharmaceutical adjuvant. The in vitro drug release study suggests that the extracted polysaccharides are potential candidates as a pharmaceutical adjuvant. Furthermore, the three isolated polysaccharides were subjected to its radical scavenging activity using DPPH, phospho molybdenum assay and reducing power assay. The results exhibited that the polysaccharides can be explored as a novel natural antioxidant and can be recommended as a functional food.

  15. Effects of extraction methods on the antioxidant activities of polysaccharides from Agaricus blazei Murrill.

    Science.gov (United States)

    Jia, Shaoyi; Li, Feng; Liu, Yong; Ren, Haitao; Gong, Guili; Wang, Yanyan; Wu, Songhai

    2013-11-01

    Five polysaccharides were obtained from Agaricus blazei Murrill (ABM) through different extraction methods including hot water extraction, single enzyme extraction (pectinase, cellulase or papain) and compound enzymes extraction (cellulase:pectinase:papain). Their characteristics such as the polysaccharide yield, polysaccharide content, protein content, infrared spectra were determined, and antioxidant activities were investigated on the basis of hydroxyl radical, DPPH free radical, ABTS free radical and reducing power. The results showed that five extracts exhibited antioxidant activities in a concentration-dependent manner. Compared with other methods, the compound enzymes extraction method was found to present the highest polysaccharides yield (17.44%). Moreover, compound enzymes extracts exhibited the strongest reducing power and highest scavenging rates on hydroxyl radicals, DPPH radicals and ABTS radicals. On the contrary, hot water extraction method had the lowest polysaccharides yield of 11.95%, whose extracts also exhibited the lowest antioxidant activities. Overall, the available data obtained in vitro models suggested that ABM extracts were natural antioxidants and compound enzymes extraction was an appropriate, mild and effective extracting method for obtaining the polysaccharide extracts from Agaricus blazei Murrill (ABM).

  16. Proteomic analysis of scallop hepatopancreatic extract provides insights into marine polysaccharide digestion

    Science.gov (United States)

    Lyu, Qianqian; Jiao, Wenqian; Zhang, Keke; Bao, Zhenmin; Wang, Shi; Liu, Weizhi

    2016-01-01

    Marine polysaccharides are used in a variety of applications, and the enzymes that degrade these polysaccharides are of increasing interest. The main food source of herbivorous marine mollusks is seaweed, and several polysaccharide-degrading enzymes have been extracted from mollusk digestive glands (hepatopancreases). Here, we used a comprehensive proteomic approach to examine the hepatopancreatic proteins of the Zhikong scallop (Chlamys farreri). We identified 435 proteins, the majority of which were lysosomal enzymes and carbohydrate and protein metabolism enzymes. However, several new enzymes related to polysaccharide metabolism were also identified. Phylogenetic and structural analyses of these enzymes suggest that these polysaccharide-degrading enzymes may have a variety of potential substrate specificities. Taken together, our study characterizes several novel polysaccharide-degrading enzymes in the scallop hepatopancreas and provides an enhanced view of these enzymes and a greater understanding of marine polysaccharide digestion. PMID:27982037

  17. Extraction and characterization of the auricularia auricular polysaccharide

    Science.gov (United States)

    Zhang, Q. T.

    2016-07-01

    To study a new protein drugs carrier, the Auricularia auricular polysaccharide (AAP) was extracted and purified from Auricularia auricular, and then characterized by the micrOTOF-Q mass spectrometer, UV/Vis spectrophotometer, moisture analyzer and SEM. The results showed that the AAP sample was water- soluble and white flocculence, its molecular weight were 20506.9 Da∼⃒63923.7 Da, and the yield, moisture, and total sugar contents of the AAP were 4.5%, 6.2% and 90.12%(w/w), respectively. The results of the SEM revealed that the AAP dried by vacuum were spherical particles with a smooth surface, and the AAP freeze-dried had continuous porous sheet shape with the loose structure.

  18. Extraction, purification, characterization and antioxidant activities of polysaccharides from Cistanche tubulosa.

    Science.gov (United States)

    Zhang, Wiejie; Huang, Jing; Wang, Wei; Li, Qian; Chen, Yao; Feng, Weiwei; Zheng, Daheng; Zhao, Ting; Mao, Guanghua; Yang, Liuqing; Wu, Xiangyang

    2016-12-01

    An efficient ultrasonic-cellulase-assisted extraction (UCE) of Cistanche tubulosa polysaccharide (CTP) was established. The response surface methodology based on Box-Behnken Design was employed to further optimize extraction conditions. After quaternary ammonium salt precipitation, the polysaccharide of C. tubulosa was characterized by different techniques. The results showed that a maximum polysaccharide yield of 22.31±0.45% was achieved at a pH of 5.2 for 31.5min at 54.1°C. Compared to hot water extraction, the yield of CTP in UCE and polysaccharide content increased to 44.96% and 70.13±2.19%, respectively. There was no marked difference among polysaccharides extracted using different methods from the infrared spectrum. Ultrasonic-cellulase-assisted extraction polysaccharide showed a fibrous structure from scanning electron microscopy and was composed of rhamnose, mannose, glucose, and galactose in a molar ratio of 2.18:1:28.29:1.43 by gas chromatography. The circular dichroism results indicated that polysaccharides had a maximum positive peak around 210nm with different peak values. The thermogravimetric analysis and differential scanning calorimetry were used to test the thermostability of CTP. Besides, CTP demonstrated appreciable antioxidant potential on antioxidant experiments in vitro. The results suggested that UCE is an effective method for CTP extraction and its polysaccharide showed appreciable antioxidant activity.

  19. Extraction of Angelica sinensis polysaccharides using ultrasound-assisted way and its bioactivity.

    Science.gov (United States)

    Zhao, Ying; Shi, Yongyong; Yang, Huixin; Mao, Lijuan

    2016-07-01

    In the present article, ultrasound-assisted extraction (UAE) of polysaccharides from Angelica sinensis were investigated. The aim of the study is to examine the extraction parameters such as ultrasound power (140-180W), the ratio of liquid to solid (5-7), extraction time (40-50min) and extraction temperature (80-100°C) and to obtain the best possible combinations of these parameters through response surface methodology (RSM). Based on contour plots and variance analysis, optimum operational conditions for maximizing polysaccharides yield were found to be 180w, 7, 45min and 90°C. Under the optimum operating conditions determined, 6.96% polysaccharides were achieved. In addition, the results showed that A. sinensis polysaccharides (ASP) could increase antioxidant enzymes activities and decrease the MDA levels in the skeletal muscle of exhaustive exercise rats. This study provides strong evidence that A. sinensis polysaccharides supplementation possessed protective effects against exhaustive exercise-induced oxidative stress.

  20. Characterization of polysaccharides extracted from spent coffee grounds by alkali pretreatment.

    Science.gov (United States)

    Ballesteros, Lina F; Cerqueira, Miguel A; Teixeira, José A; Mussatto, Solange I

    2015-01-01

    Spent coffee grounds (SCG), obtained during the processing of coffee powder with hot water to make soluble coffee, are the main coffee industry residues and retain approximately seventy percent of the polysaccharides present in the roasted coffee beans. The purpose of this study was to extract polysaccharides from SCG by using an alkali pretreatment with sodium hydroxide at 25°C, and determine the chemical composition, as well as the antioxidant and antimicrobial properties of the extracted polysaccharides. Galactose (60.27%mol) was the dominant sugar in the recovered polysaccharides, followed by arabinose (19.93%mol), glucose (15.37%mol) and mannose (4.43%mol). SCG polysaccharides were thermostable, and presented a typical carbohydrate pattern. Additionally, they showed good antioxidant activity through different methods and presented high antimicrobial percent inhibition against Phoma violacea and Cladosporium cladosporioides (41.27% and 54.60%, respectively). These findings allow identifying possible applications for these polysaccharides in the food industry.

  1. Microwave assisted extraction of sulfated polysaccharides (fucoidan) from Ascophyllum nodosum and its antioxidant activity.

    Science.gov (United States)

    Yuan, Yuan; Macquarrie, Duncan

    2015-09-20

    Sulfated polysaccharides (fucoidan) from brown seaweed Ascophyllum nodosum were extracted by microwave assisted extraction (MAE) technology. Different conditions of temperature (90-150°C), extraction time (5-30 min) were evaluated and optimal fucoidan yield was 16.08%, obtained from 120°C for 15 min's extraction. Compositional analysis, GPC, HPAEC and IR analysis were employed for characterization of extracted sulfated polysaccharides. Fucose was the main monosaccharide of fucoidan extracted at 90°C while glucuronic acid was the main monosaccharide of fucoidan extracted at 150°C. Both the molecular weight and sulfate content of extracted fucoidan increased with decreasing extraction temperature. All fucoidans exhibited antioxidant activities as measured by DPPH scavenging and reducing power, among which fucoidan extracted at 90°C was highest. This study shows that MAE is an efficient technology to extract sulfated polysaccharides from seaweed and Ascophyllum nodosum could potentially be a resource for natural antioxidants.

  2. Arabinose and ferulic acid rich pectic polysaccharides extracted from sugar beet pulp.

    NARCIS (Netherlands)

    Oosterveld, A.; Beldman, G.; Schols, H.A.; Voragen, A.G.J.

    1996-01-01

    Arabinose and ferulic acid rich polysaccharides were extracted from sugar beet pulp using two extraction methods: a sequential extraction with H2O (2 times), NaOH/EDTA (2 times), and 4 M NaOH (2 times; method A) and a sequential extraction in which the NaOH/EDTA extraction was replaced by an

  3. Extraction of water-soluble polysaccharide and the antioxidant activity from Semen cassiae

    Directory of Open Access Journals (Sweden)

    Changjian Liu

    2014-12-01

    Full Text Available Water-soluble polysaccharide was isolated from Semen cassiae using water for extraction and ethanol for deposition. The optimized conditions for polysaccharide isolation by orthogonal experiments were a sample to liquid ratio of 1:30 at 80°C for 3.5 hours; the yield of polysaccharide from Semen cassiae under these conditions was 5.46%. Different polysaccharides (SCPW-1, SCPW-2, SCPW-3, SCPW-4, SCPW-5, SCPS-1, SCPS-2 were obtained from the extract (i.e., crude polysaccharide by DEAE-cellulose column chromatography. The polysaccharides obtained showed different structures by Fourier transform infrared therein the five elected from the seven kinds separated. The antioxidant activities of the extract were evaluated. The scavenging rates of the present extract on hydroxyl and superoxide were 43.32% and 64.97%, respectively, at a concentration of polysaccharide of 94.03 μg/mL, which was better than vitamin C at the same concentration. The scavenging rate of the present extract on 1,1-diphenyl-2-picrylhydrazyl was 13.33% at a polysaccharide concentration of 94.03 μg/mL, which was less than vitamin C at the same concentration.

  4. Alkali extraction and in vitro antioxidant activity of Monascus mycelium polysaccharides

    OpenAIRE

    Wang, Pengrong; Chen, Danfeng; Jiang, Donghua; Dong, Xiameng; Chen, Panpan; Lin, Yaoxue

    2012-01-01

    In the present work, alkali extraction technology was used to optimize the extraction of Monascus mycelium polysaccharides for the first time. The extracting parameters of alkali extracted Monascus mycelium polysaccharides were optimized by Box-Behnken design (BBD). The optimum conditions were extraction temperature 49 °C, alkali concentration 7%, solvent/material ratio 23:1 (ml/g) and extraction time 2.3 h with an enhanced yield of 10.1%, compared with the yield 4.76% of hot water extraction...

  5. Alkali extraction and in vitro antioxidant activity of Monascus mycelium polysaccharides.

    Science.gov (United States)

    Wang, Pengrong; Chen, Danfeng; Jiang, Donghua; Dong, Xiameng; Chen, Panpan; Lin, Yaoxue

    2014-07-01

    In the present work, alkali extraction technology was used to optimize the extraction of Monascus mycelium polysaccharides for the first time. The extracting parameters of alkali extracted Monascus mycelium polysaccharides were optimized by Box-Behnken design (BBD). The optimum conditions were extraction temperature 49 °C, alkali concentration 7%, solvent/material ratio 23:1 (ml/g) and extraction time 2.3 h with an enhanced yield of 10.1%, compared with the yield 4.76% of hot water extraction, indicating that alkali extraction is a more efficient way. In order to discuss the biological activity of alkali extracted polysaccharides, we compared the in vitro antioxidant activity of alkali extracted polysaccharides (AMP) with hot water extracted polysaccharides (HMP). The result showed that AMP have the similar capability of scavenging both superoxide radical and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical of HMP in vitro. Therefore, alkali extraction technology is not only a high-efficiency way to extract AMP, but also can retain the natural antioxidant activities of AMP, which can be used in pharmaceutical and food industries.

  6. Apoptosis of multiple myeloid cells induced by polysaccharides extracts from Hedyotis diffusa and its mechanism

    Institute of Scientific and Technical Information of China (English)

    林圣云

    2013-01-01

    Objective To explore the proliferation inhibition and apoptosis effects of polysaccharides extracts from Hedyotis diffusa(PEHD)on multiple myeloma(MM) cell line RPMI 8226 cells in vitro,so as to provide experimental

  7. Enzyme assisted extraction of polysaccharides from the fruit of Cornus officinalis.

    Science.gov (United States)

    You, Qinghong; Yin, Xiulian; Zhao, Yuping

    2013-10-15

    Process of enzyme assisted extraction (EAE) of polysaccharides from Cornus officinalis was optimized by response surface methodology (RSM). The influence of four different factors on the yield of C. officinalis polysaccharides (COP) was studied. Results showed that the optimal conditions were compound enzyme amount of 2.15%, extraction pH of 4.2, extraction temperature of 55 °C and extraction time of 97 min. Under these conditions, the COP yield was 9.29±0.31%, which was well in agreement with the value predicted by the model. The three methods, EAE, hot water extraction (HWE), ultrasound-assisted extraction (UAE) for extracting COP by RSM were further compared. Results showed that EAE had the largest yield of polysaccharides with lower equipment cost.

  8. Extraction and Characterization of Tamarind (Tamarind indica L.) Seed Polysaccharides (TSP) from Three Difference Sources

    OpenAIRE

    Khanittha Chawananorasest; Patsuda Saengtongdee; Praphakorn Kaemchantuek

    2016-01-01

    Tamarind seed polysaccharide (TSP), a natural polysaccharide extracted from tamarind seeds is used in the pharmaceutical, textile and food industries as a mucoadhesive polymer. This work aimed to extract TSP from tamarind seeds from three sources with two methods and characterized its physical and chemical properties. Kernel powder of tamarind seeds was slurried into a clear solution, set aside overnight and then centrifuged at 6000 rpm for 20 min to separate all foreign matter. The supernata...

  9. Sulfated Polysaccharides in Marine Sponges: Extraction Methods and Anti-HIV Activity

    Directory of Open Access Journals (Sweden)

    Ana I. S. Esteves

    2011-01-01

    Full Text Available The extraction, fractionation and HIV-1 inhibition potential of polysaccharides extracted from three species of marine sponges, Erylus discophorus, Cliona celata and Stelletta sp., collected in the Northeastern Atlantic, is presented in this work. The anti-HIV activity of 23 polysaccharide pellets and three crude extracts was tested. Crude extracts prepared from Erylus discophorus specimens were all highly active against HIV-1 (90 to 95% inhibition. Cliona celata pellets showed low polysaccharide content (bellow 38.5% and almost no anti-HIV activity (<10% inhibition. Stelletta sp. pellets, although quite rich in polysaccharide (up to 97.3%, showed only modest bioactivity (<36% HIV-1 inhibition. Erylus discophorus pellets were among the richest in terms of polysaccharide content (up to 98% and the most active against HIV-1 (up to 95% inhibition. Chromatographic fractionation of the polysaccharide pellet obtained from a specimen of Erylus discophorus (B161 yielded only modestly active fractions. However, we could infer that the active molecule is most probably a high molecular weight sulfated polysaccharide (>2000 kDa, whose mechanism is possibly preventing viral attachment and entry (fusion inhibitor.

  10. Antioxidant activity of polysaccharide extracted from Ganoderma lucidum using response surface methodology.

    Science.gov (United States)

    Kan, Yongjun; Chen, Tiqiang; Wu, Yanbin; Wu, Jianguo; Wu, Jinzhong

    2015-01-01

    Superfine grinding technology was applied for polysaccharide extraction from the fruiting bodies of Ganoderma lucidum, and response surface methodology (RSM) was used to optimize the effects of processing parameters on polysaccharide extraction yield. Results showed that the maximum yield of G. lucidum polysaccharides (GLP) was obtained at an optimum condition: extraction time 137 min, extraction temperature 66 ̊C, the ratio of water to material 35 mL/g, and the GLP extracting yield reached 2.44% under this condition. GLP were precipitated into three crude polysaccharides, viz. GLP40, GLP60 and GLP80. The basic characterization of polysaccharides was determined by using HPLC and FT-IR methods. GLP, GLP80, GLP60, and GLP40 were composed of Man, Rib, Glc, Gal and Fuc with the molar ratios of 1.27:0.36:22.89:1.61:0.33, 1.40:0.31:23.02:3.46:0.91, 0.96:0.34:25.76:2.47:0.46, and 2.81:1.42:23.83:1.61:0.33, respectively. The result of FT-IR suggested that the monosaccharide residue of the four polysaccharides was β-pyranoid ring. Moreover, the antioxidant activities of these four polysaccharides were evaluated. The results showed that GLP80 had the best reducing power, DPPH radical scavenging ability and oxygen radical scavenging ability followed by GLP, GLP60 and GLP40. Our results demonstrated that RSM might be a valuable technique for optimizing the efficient extraction of GLP, and G. lucidum could be considered as sources of natural antioxidants and preservatives of food industry. Moreover, polysaccharides, especially GLP80, extracted from the fruiting bodies of G. lucidum, exhibited promising antioxidant activities.

  11. Effect of extraction method on structure and antioxidant activity of Hohenbuehelia serotina polysaccharides.

    Science.gov (United States)

    Li, Xiaoyu; Wang, Lu

    2016-02-01

    The impacts of four extraction methods (hot water, enzyme assistance, ultrasonic assistance and ultrasonic-enzyme assistance) on the extraction yields, preliminary structure and antioxidant activities of the Hohenbuehelia serotina polysaccharides (HW-HSP, EA-HSP, UA-HSP and UEA-HSP) were systematically investigated. The yield of the polysaccharides (20.70±0.17%) obtained by ultrasonic-enzyme assistance was higher than the polysaccharides by other methods'. Four kinds of polysaccharides possessed the different preliminary structural characteristics including molecular weight distributions, monosaccharide compositions, crystallization and spiral structures, while different surface morphology. Through the measurements of antioxidant activities in vitro, UEA-HSP exhibited the most significant scavenging capacities on non-physiological ABTS free radicals and physiological hydroxyl radicals. These data showed that ultrasonic-enzyme assistance was more beneficial to enhance the extraction yields of the polysaccharides, and obtain higher bioactive polysaccharides. The results also suggested that H. serotina polysaccharides possessed potential healthcare application in food field due to their antioxidant activities.

  12. Extraction, Structural Characterization, and Potential Antioxidant Activity of the Polysaccharides from Four Seaweeds

    Directory of Open Access Journals (Sweden)

    Jinzhe He

    2016-11-01

    Full Text Available Four seaweed polysaccharides were extracted from Sarcodia ceylonensis, Ulva lactuca L., Gracilaria lemaneiformis, and Durvillaea antarctica, respectively, by microwave-assisted extraction. The effect of three significant variables (extraction time, extraction temperature, and the ratio of water to raw material on the process for extracting polysaccharides was investigated, along with the optimization of the extraction using the response surface method (RSM with a Box–Behnken design. The polysaccharide structure, monosaccharide composition, degree of sulfation, and molecular weight (MW distribution were analyzed by infrared (IR spectrometry, gas chromatography (GC, and high-performance gel permeation chromatography (HPGPC. IR spectrometry showed that Sarcodia ceylonensis polysaccharide (SCP, Ulva lactuca L. polysaccharide (ULLP, and Durvillaea antarctica polysaccharide (DAP were all sulfated polysaccharides and, except Gracilaria lemaneiformis polysaccharide (GLP, all belong to β-pyranosidic polysaccharides. The average molecular weight (MW of SCP, ULLP, GLP, and DAP was 466, 404, 591, and 482 kDa, respectively. The quantitative and comparative results with external standards indicated that the main monosaccharide in SCP and ULLP was mannose; and GLP and DAP were mainly composed of galactose and glucose, respectively. Then the in vitro antioxidant activity of all of the polysaccharides was evaluated using different assays—2,2–azino –bis (3-ethylbenzthiazoline-6- sulfonate (ABTS, hydroxyl radical, nitrite scavenging capacity, and reducing power—and the relationship between their antioxidant activity and chemical characteristics were also examined. ULLP presented the highest ABTS radical scavenging activity; ULLP, SCP and DAP also showed a strong effect on the ABTS radical scavenging activity. SCP and ULLP exhibited excellent hydroxyl radical scavenging activities, about 83.33% ± 2.31% and 80.07% ± 2.17%, respectively, at 4 mg/mL. The

  13. Extraction, Structural Characterization, and Potential Antioxidant Activity of the Polysaccharides from Four Seaweeds.

    Science.gov (United States)

    He, Jinzhe; Xu, Yaoyang; Chen, Hongbo; Sun, Peilong

    2016-11-28

    Four seaweed polysaccharides were extracted from Sarcodia ceylonensis, Ulva lactuca L., Gracilaria lemaneiformis, and Durvillaea antarctica, respectively, by microwave-assisted extraction. The effect of three significant variables (extraction time, extraction temperature, and the ratio of water to raw material) on the process for extracting polysaccharides was investigated, along with the optimization of the extraction using the response surface method (RSM) with a Box-Behnken design. The polysaccharide structure, monosaccharide composition, degree of sulfation, and molecular weight (MW) distribution were analyzed by infrared (IR) spectrometry, gas chromatography (GC), and high-performance gel permeation chromatography (HPGPC). IR spectrometry showed that Sarcodia ceylonensis polysaccharide (SCP), Ulva lactuca L. polysaccharide (ULLP), and Durvillaea antarctica polysaccharide (DAP) were all sulfated polysaccharides and, except Gracilaria lemaneiformis polysaccharide (GLP), all belong to β-pyranosidic polysaccharides. The average molecular weight (MW) of SCP, ULLP, GLP, and DAP was 466, 404, 591, and 482 kDa, respectively. The quantitative and comparative results with external standards indicated that the main monosaccharide in SCP and ULLP was mannose; and GLP and DAP were mainly composed of galactose and glucose, respectively. Then the in vitro antioxidant activity of all of the polysaccharides was evaluated using different assays-2,2-azino -bis (3-ethylbenzthiazoline-6- sulfonate) (ABTS), hydroxyl radical, nitrite scavenging capacity, and reducing power-and the relationship between their antioxidant activity and chemical characteristics were also examined. ULLP presented the highest ABTS radical scavenging activity; ULLP, SCP and DAP also showed a strong effect on the ABTS radical scavenging activity. SCP and ULLP exhibited excellent hydroxyl radical scavenging activities, about 83.33% ± 2.31% and 80.07% ± 2.17%, respectively, at 4 mg/mL. The reducing power of

  14. Characterization of Polysaccharide by HPLC: Extraction and Anticancer Effects

    OpenAIRE

    Liming Gao; Ya Di; Jiandong Wu; Ming Shi; Fulu Zheng

    2014-01-01

    Cervical cancer is a serious health hazard for women’s reproductive system cancer; the method of treatment for cervical cancer is still in surgery, chemotherapy, and radiotherapy as the basic means, but with many complications. The effects of natural medicines for cervical cancer are increasingly becoming the focus of people’s attentions. By studying the polysaccharide of cervical cancer in mice, we found that shark cartilage polysaccharide can increase the serum levels of T-SOD and GSH and d...

  15. Optimization extraction, preliminary characterization and antioxidant activity in vitro of polysaccharides from Stachys sieboldii Miq. tubers.

    Science.gov (United States)

    Feng, Kai; Chen, Wei; Sun, Liwei; Liu, Jianzeng; Zhao, Yangxin; Li, Luxi; Wang, Yuxing; Zhang, Wenjing

    2015-07-10

    Response surface methodology was used to optimize the extraction conditions of water-soluble polysaccharides from Stachys sieboldii Miq. tubers. A central composite design was used to optimize the extraction processing parameters. The optimum extraction conditions are as follows: extraction temperature, 95°C; extraction time, 2.5h; water to raw material ratio, 16; and extraction frequency, 3. Under the optimized conditions, an experimental yield of 9.21 ± 0.18%, which is in good agreement with the predicted yield, was obtained. Purified polysaccharide SSP II-a was successfully obtained using diethylaminoethanol-Sepharose and Sepharose CL-6B column chromatography. SSP II-a was found to be an acidic polysaccharide fraction with an average molecular weight of 168kDa and composed of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose. In vitro antioxidant activity assays suggested that SSP II-a presents high scavenging activity toward superoxide anion, hydroxyl, and 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) radicals but relatively lower scavenging activity toward 1,1-diphenyl-2-picrylhydrazyl radicals. The results indicated that response surface methodology is an effective method for the extraction of polysaccharides from S. sieboldii Miq. tubers and the polysaccharides could be explored as a potential antioxidant agent for use in medicine or functional food.

  16. Optimization of PEG-based extraction of polysaccharides from Dendrobium nobile Lindl. and bioactivity study.

    Science.gov (United States)

    Zhang, Yi; Wang, Hongxin; Wang, Peng; Ma, ChaoYang; He, GuoHua; Rahman, Md Ramim Tanver

    2016-11-01

    Polyethylene glycol (PEG) as a green solvent was employed to extract polysaccharide. The optimal conditions for PEG-based ultrasonic extraction of Dendrobium nobile Lindl. polysaccharide (JCP) were determined by response surface methodology. Under the optimal conditions: extraction temperature of 58.5°C; ultrasound power of 193W, and the concentration of polyethylene glycol-200 (PEG-200) solution of 45%, the highest JCP yield was obtained as 15.23±0.57%, which was close to the predicted yield, 15.57%. UV and FT-IR analysis revealed the general characteristic absorption peaks of both JCP with water extraction (JCPw) and PEG-200 solvent extraction (JCPp). Thermal analysis of both JCPs was performed with Thermal Gravimetric Analyzer (TGA) and Differential Scanning Calorimeter (DSC). Antioxidant activities of two polysaccharides were also compared and no significant difference in vitro was obtained. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Optimization of enzyme-assisted extraction of polysaccharides from alfalfa and its antioxidant activity.

    Science.gov (United States)

    Wang, Shaopu; Dong, Xiaofang; Tong, Jianming

    2013-11-01

    In this present study, an efficient complex enzyme-assisted extraction technology was developed and optimized to extract polysaccharides from alfalfa using four factors at five levels central composite rotatable response surface design (CCRD). The experimental data was fitted to a second order polynomial equation with high coefficient of determination values (R(2)>0.95). The results of statistical analysis showed that the linear and quadratic terms of these four variables had significant effects (Penzyme concentration of 2.5%, 2.0%, 3.0% (weight of alfalfa) of cellulase, papain and pectase, extraction temperature 52.7 °C, extraction pH 3.87, ratio of water to raw material 78.92 mL/g and extraction time 2.73 h. Under the optimal conditions, the experimental extraction yield of alfalfa polysaccharides was 5.05 ± 0.02%, which was well matched with the value (5.09%) predicted by the CCRD model. Moreover, evaluation of the antioxidant activity of polysaccharides from alfalfa in vitro suggested that the polysaccharides had good antioxidant effect, especially scavenging activity for hydroxyl radical and DPPH radical, which indicated that the polysaccharides from alfalfa may be explored as a novel natural antioxidant.

  18. Cellulase-assisted extraction of polysaccharides from Cucurbita moschata and their antibacterial activity.

    Science.gov (United States)

    Qian, Zhi-Gang

    2014-01-30

    In this study, cellulase-assisted extraction of water soluble polysaccharides from pumpkin (Cucurbita moschata) and their antibacterial activity were investigated. The polysaccharides yield was monitored during the extraction process. The optimum extraction conditions were determined as follows: time, 40 min; temperature, 55°C; pH, 4.5; and cellulase amount, 4,000 U/g. The extracts were centrifuged, filtered, proteins removed by Sevag method, concentrated to approximately 15% (w/v), precipitated with 5 volumes of absolute ethanol, freeze-dried, and pulverized to yield a water soluble powder of pumpkin polysaccharides (PP). The sugar content of the product was 68.3%, and the yield was 17.34% (w/w), respectively. The PP had high antibacterial activity against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli at the concentration of 100 mg/mL.

  19. Optimization of ultrasonic-assisted extraction and in vitro antioxidant activities of polysaccharides from Trametes orientalis.

    Science.gov (United States)

    Zheng, Yi; Li, Yong; Wang, Wei-dong

    2014-10-13

    A Box-Behnken design was employed to optimize ultrasonic-assisted extraction of Trametes orientalis polysaccharides (TOP). The crude polysaccharides were purified by DEAE cellulose-52 chromatography, giving a main fraction named as PTOP. The antioxidant properties of PTOP were evaluated by different in vitro antioxidant assays, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities, reducing power, superoxide radical scavenging activities, and chelating ability of ferrous ions. The results showed that optimal extraction parameters were as follows: ratio of water to raw material 30.6 mL/g, ultrasonic power 109.8 W, extraction temperature 40.2 °C, and extraction time 42.2 min. Under these conditions, the experimental yield of polysaccharides was 7.49 ± 0.14%, which agreed closely with the predicted value (7.47%). Furthermore, PTOP exhibited antioxidant capacity in a concentration-dependent manner in all assays.

  20. Optimum extraction of polysaccharides from Opuntia dillenii and evaluation of its antioxidant activities.

    Science.gov (United States)

    Yang, Qin; Chen, Huaguo; Zhou, Xin; Zhang, Junzeng

    2013-09-12

    Response surface methodology (RSM) was applied to optimize the extraction of crude polysaccharides from Opuntia dillenii (Ker-Gaw) Haw. A three-level, four-variable Box-Behnken design was employed to obtain the best possible combination of extraction temperature (80-90 °C), extraction time (50-70 min), number of extraction cycle (1-3 times), and ratio of water to raw material (8:1-12:1, v/w) for maximum yield of crude polysaccharide. Besides, the antioxidant capacity of crude polysaccharide was evaluated by DPPH assay. The results showed that optimized extraction conditions were extraction temperature 85 °C, extraction time 63.7 min, extraction 2 times and ratio of water to raw material 11.14:1. Under these conditions, the experimental yield was 27.36 ± 0.21%, which is well in close agreement with the value (27.44%) predicted by RSM model. Pharmacological test showed that O. dillenii crude polysaccharides had a good antioxidant activity.

  1. Beiwei ZHU%Optimization of Enzyme Extraction Conditions of Cordycepin Polysaccharide Using Response Surface Methodology

    Institute of Scientific and Technical Information of China (English)

    Dong AN; Beiwei ZHU

    2012-01-01

    [Objective] This study aimed to optimize extraction from the leftover of Cordyceps militaris the conditions for polysaccharide culture medium. [Method] Firstly the compositions of C. militaris culture medium were detected, before the cordycepin polysaccharide in medium was extracted using enzymatic hydrolysis. The optimum hydrolytic enzyme was selected through single factor test. Then, the extraction tem- perature, pH, enzyme content and solid-liquid ratio were optimized by response sur- face methodology, and confirmed by mathematical simulation. [Result] Acid hydrolytic enzyme was the optimum for extracting polysaccharides from the leftover of C. mili- taris culture medium. The optimum extraction conditions were as follows: extraction temperature 39.89 ℃, solid-liquid ratio 1:75.78, enzyme content 2.39% and pH 3.12. Under these conditions, the extraction rate of polysaccharides reached 9.96%. [Con- clusion] The study could provide a certain theoretical direction for extracting polysac- charities from the leftover of C. mliltaris culture medium on a large scale.

  2. Extraction and characterization of polysaccharides from green and roasted Coffea arabica beans.

    NARCIS (Netherlands)

    Oosterveld, A.; Harmsen, H.; Voragen, A.G.J.; Schols, H.A.

    2003-01-01

    Polysaccharides were sequentially extracted from green and roasted Coffea arabica beans with water (90 °C), EDTA, 0.05, 1, and 4 M NaOH and characterized chemically. Additionally, the beans were subjected to a single extraction with water at 170 °C. Green arabica coffee beans contained large proport

  3. Cellulase-assisted extraction, characterization, and bioactivity of polysaccharides from Polygonatum odoratum.

    Science.gov (United States)

    Liu, Xin; Zhang, Miansong; Guo, Kai; Jia, Airong; Shi, Yaping; Gao, Ganglong; Sun, Zhenliang; Liu, Changheng

    2015-04-01

    Cellulase-assisted extraction of polysaccharides from Polygonatum odoratum (CPP) was optimized by response surface methodology (RSM) and the extracted CPP's preliminary chemical characteristics, as well as antioxidant and immunomodulatory activities were also investigated. The optimal extraction parameters comprised an extraction temperature of 58.21 °C, an extraction time of 3.18 h, pH value of 5.8, and cellulase amount of 6.0%. Under these conditions, the relative yield was 15.76%, higher than the yield achieved with hot water extracted polysaccharide (HPP). Chemical composition analysis demonstrated that CPP and HPP consisted of mannose, glucosamine, rhamnose, glucose, galactose, and arabinose with a molecular ratio of 7.80:1.08:1.63:65.93:3.58:1.00 and 11.22:0.23:0.23:17.59:2.73:9.10, respectively. The molecular weight distribution of CPP was lower and more homogeneous compared with HPP. CPP exhibited stronger antioxidant activities than HPP, including DPPH radical scavenging activity and reducing power. Both CPP and HPP could significantly promote the proliferation and neutral red phagocytosis of RAW 264.7 macrophage cells in vitro. These results indicate that the cellulase-assisted extraction method influenced the physicochemical characteristics, and consequently, the functional activities of polysaccharides, suggesting the cellulose-assisted method may be a viable option for extraction polysaccharides from P. odoratum.

  4. Some Physical Properties of Protein Moiety of Alkali-Extracted Tea Polysaccharide Conjugates Were Shielded by Its Polysaccharide

    Directory of Open Access Journals (Sweden)

    Xiaoqiang Chen

    2017-05-01

    Full Text Available Polysaccharide conjugates were alkali-extracted from green tea (TPC-A. Although it contained 11.80% covalently binding proteins, TPC-A could not bind to the Coomassie Brilliant Blue dyes G250 and R250. TPC-A had no expected characteristic absorption peak of protein in the UV-vis spectrum scanning in the range of 200–700 nm. The UV-vis wavelength of 280 nm was not suitable to detect the presence of the protein portion of TPC-A. The zeta potential of TPC-A merely presented the negative charge properties of polysaccharides instead of the acid–base property of its protein section across the entire pH range. Furthermore, TPC-A was more stable when the pH of solution exceeded 4.0. In addition, no precipitation or haze was generated in the TPC-A/(−-epigallocatechin gallate (EGCG mixtures during 12 h storage. TPC-A has emulsifying activity, which indicated that its protein moiety formed hydrophobic groups. Thus, it was proposed that some physical properties of TPC-A protein were shielded by its olysaccharide, since the protein moiety was wrapped by its polysaccharide chains.

  5. Optimum extraction of polysaccharides from motherwort leaf and its antioxidant and antimicrobial activities.

    Science.gov (United States)

    Tahmouzi, Saeed; Ghodsi, Mitra

    2014-11-04

    Box-Behnken design was employed to optimize the extraction conditions for polysaccharides from the leaves of motherwort (Leonurus cardiaca L.). Three independent variables including extraction temperature (60-100 °C), extraction time (60-120 min), and the ratio of water to raw material (20-60) were investigated. The results revealed that the quadratic and linear terms of three factors had strong effects on the extraction yield of polysaccharides from motherwort leaf. The best extraction conditions for the yield of polysaccharide (LCLP) was extraction temperature of 81.4 °C, time of 106.6 min and the ratio of water to raw material of 45.2. Under the optimal conditions, the extraction yield of LCLP was 9.17 ± 0.39%, which was well matched with value predicted by the model 9.26%. The results indicated that the purified LCLP exerted obvious scavenging effects on free radicals in vitro. Furthermore, motherwort polysaccharides could be used as a novel antimicrobial additive. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. Hydrothermal extraction and micronization of polysaccharides from Ganoderma lucidum in a one-step process

    Directory of Open Access Journals (Sweden)

    Wahyudiono

    2013-02-01

    Full Text Available Ganoderma lucidum (G. lucidum is a mushroom-forming white rot fungus that contains a wide variety of bioactive components (glucans. In this study, G. lucidum was utilized for the extraction of polysaccharides by hot compressed water at a temperature of 160oC and a pressure of 4.0 MPa using a semi-batch system. Under these conditions, thermal softening of G. lucidum occurred, allowing the removal of the polysaccharides protecting other constituents in G. lucidum via hydrolysis. Next, the extract was directly atomized by spray drying to remove the water. Scanning electron microscope (SEM images showed that the particles formed were spherical and dimpled or shriveled with diameters varying from 1 to 6 m. Based on these results it is proposed that this process is applicable to isolate polysaccharides from other types of biomass and may result in advances in extraction technology to obtain plant biomass components.

  7. Extraction and separation of polysaccharides from Laminaria japonica by size-exclusion chromatography.

    Science.gov (United States)

    Zhang, Heng; Row, Kyung Ho

    2015-04-01

    A large number of studies have suggested that polysaccharides, such as fucoidan and laminarin, in various seaweeds have significant biological properties. A different distribution of molecular weights is a prominent sign of many polysaccharides. Therefore, a simple, fast and reliable high-performance size-exclusion chromatography (HPSEC) method was proposed to separate fucoidan and laminarin from Laminaria japonica. After evaluating the different separation conditions for HPSEC, such as the type of mobile phase and flow rate, an acid extraction method was established and optimized by a systematic investigation of the influencing factors. Under the optimal conditions, 169.2 and 383.8 mg g(-1) of fucoidan and laminarin, respectively, were extracted. This method is suitable for the extraction and separation of polysaccharides with good reproducibility of the retention time, acceptable linearity, small relative standard deviation and low detection limits. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  8. Anti-inflammatory and angiogenic activity of polysaccharide extract obtained from Tibetan kefir.

    Science.gov (United States)

    Prado, Maria Rosa Machado; Boller, Christian; Zibetti, Rosiane Guetter Mello; de Souza, Daiany; Pedroso, Luciana Lopes; Soccol, Carlos Ricardo

    2016-11-01

    The search for new bioactive molecules is a driving force for research pharmaceutical industries, especially those molecules obtained from fermentation. The molecules possessing angiogenic and anti-inflammatory attributes have attracted attention and are the focus of this study. Angiogenic activity from kefir polysaccharide extract, via chorioallantoic membrane assay, exhibited a pro-angiogenic effect compared with vascular endothelial factor (pro-angiogenic) and hydrocortisone (anti-angiogenic) activity as standards with an EC50 of 192ng/mL. In terms of anti-inflammatory activity determined via hyaluronidase enzyme assay, kefir polysaccharide extract inhibited the enzyme with a minimal activity of 2.08mg/mL and a maximum activity of 2.57mg/mL. For pharmaceutical purposes, kefir polysaccharide extract is considered to be safe because it does not inhibit VERO cells in cytotoxicity assays. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. Determination of Polysaccharide in Radix pseudostellariae Extract by Size-Exclusion High-Performance Liquid Chromatography

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A simple size-exclusion high-performance liquid chromatographic method was developed for rapid molecular mass screening to determine the average molecular mass, polydispersity, and quantity of Taizishen polysaccharides extract. The screening used a TOSOH TSK-GEL G3000SW high-performance liquid chromatographic column with 0.1 mol/L NaNO3 buffer as the mobile phase and refractive index detector. The molecular mass calibration curve was linear for polysaccharide standards from 10 to 100 kDa with a correlation coefficient of 0.9991. The method can be used to analyze the quantity, average molecular mass,and polydispersity of polysaccharides. In addition, the screening method is suitable for quality control of polysaccharide preparations in Chinese medicines and pharmaceutical products.

  10. Extraction and characterization of three polysaccharides extracted from Opuntia ficus indica cladodes.

    Science.gov (United States)

    Bayar, Nadia; Kriaa, Mouna; Kammoun, Radhouane

    2016-11-01

    The chemical extraction and the characterization of polysaccharides from mucilage (MC), pectin (PC) and total pectic mucilage fraction (TFC) of Opuntia ficus indica cladodes as well as the evaluation of their antioxidant activities was investigated. The FTIR spectroscopic analysis revealed the presence of carboxyl and hydroxyl groups corresponding to polysaccharides. Uronic acid and the total sugar contents of PC were higher than those of TFC and MC whereas ash content of MC was considerably more important. In addition, the findings showed that all the samples had little protein content and low average molecular weight compared to the results mentioned in literature. Furthermore, MC reached not only the highest water (WHC) and oil holding (OHC) capacities (7.81g/g and 1.34g/g, respectively) but also the highest antioxidant properties (DPPH and ABTS scavenging activities, β-carotene bleaching inhibition activity and reducing power). However, PC had the strongest emulsifying and foaming properties. As for TFC, it had low WHC, OHC and emulsifying properties whereas it had higher foaming properties than MC and greater antioxidant properties compared to PC. These outcomes can encourage the use of PC as a surfactant and MC and TFC as natural antioxidants in food and pharmaceutical industries.

  11. Optimization of extraction process and investigation of antioxidant effect of polysaccharides from the root of Limonium sinense Kuntze

    OpenAIRE

    2011-01-01

    Objective: To optimize the extraction technology for polysaccharides from the root of Limonium sinense (Girard) Kuntze, Plumbaginaceae and evaluate the antioxidant capacity of polysaccharides from L. sinense (LSEP) Materials and Methods : One-singer factor and response surface methodology(RSM) were established to extract the polysaccharides from L. sinense. Then, the 1,1-diphenyl-2-picrylhydrazyl free radical, hydroxyl radical(.OH), and 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) ...

  12. Extraction, Characterization, and Molecular Weight Determination of Senna tora (L. Seed Polysaccharide

    Directory of Open Access Journals (Sweden)

    Harshal A. Pawar

    2015-01-01

    Full Text Available The objective of the present work was extraction of polysaccharide from Senna tora L. seed and its characterization as a pharmaceutical excipient. Polysaccharide extraction was based on mechanical separation of the endosperm of seeds of Senna tora, water dissolution, centrifugation, and precipitation with acetone. Standard procedures were used to study the viscosity, micromeritic properties, and microbial bioburden. Accelerated stability study was carried out on isolated polysaccharide for six months at 40°C/75 RH as per ICH guidelines. The gum obtained from S. tora seeds was an amorphous free flowing odourless powder with dull brown colour (yield = 35% w/w. The bulk density, tapped density, and angle of repose data reveal that S. tora gum possesses good flow property. The intrinsic viscosity obtained was 1.568 dL/g. The average molecular weight of purified S. tora gum was found to be 198 kDa by intrinsic viscosity method. The results indicated that viscosity of gum solution increases with increase in temperature. FTIR study revealed the absence of degradation or decomposition of polysaccharide at accelerated stability conditions for six months. It has been concluded that extracted polysaccharide can be used as pharmaceutical excipient in terms of flow behavior, microbial properties, and stability.

  13. Optimization of enzyme-assisted extraction and characterization of polysaccharides from Hericium erinaceus.

    Science.gov (United States)

    Zhu, Yang; Li, Qian; Mao, Guanghua; Zou, Ye; Feng, Weiwei; Zheng, Daheng; Wang, Wei; Zhou, Lulu; Zhang, Tianxiu; Yang, Jun; Yang, Liuqing; Wu, Xiangyang

    2014-01-30

    The enzyme-assisted extraction (EAE) of polysaccharides from the fruits of Hericium erinaceus was studied. In this study, response surface methodology and the Box-Behnken design based on single-factor and orthogonal experiments were applied to optimize the EAE conditions. The optimal extraction conditions were as follows: a pH of 5.71, a temperature of 52.03°C and a time of 33.79 min. The optimal extraction conditions resulted in the highest H. erinaceus polysaccharides (HEP) yield, with a value 13.46 ± 0.37%, which represented an increase of 67.72% compared to hot water extraction (HWE). The polysaccharides were characterized by FT-IR, SEM, CD, AFM, and GC. The results showed that HEP was composed of mannose, glucose, xylose, and galactose in a molar ratio of 15.16:5.55:4.21:1. The functional groups of the H. erinaceus polysaccharides extracted by HWE and EAE were fundamentally identical but had apparent conformational changes.

  14. Extraction optimization of polysaccharide from Zanthoxylum bungeanum using RSM and its antioxidant activity.

    Science.gov (United States)

    Li, Peiqin; Zhou, Ligang; Mou, Yan; Mao, Ziling

    2015-01-01

    Polysaccharide prepared from pericarp of Zanthoxylum bungeanum was proved to possess excellent antioxidant activities in vitro by using reducing ferric iron power, DPPH radical scavenging activity, chelating ferrous iron capacity, and hydroxyl radical scavenging activity assays in the present study. In those four antioxidant assay models, Z. bungeanum polysaccharide (ZBP) displayed prominent antioxidant activities with low EC50 values of 0.011, 0.021, 0.056 and 0.008 mg/mL, respectively. Moreover, the extraction process of ZBP was further optimized by response surface methodology combined with Box-Behnken design. The highest polysaccharide yield 13.96%, which agreed closely with the predicted yield 13.20%, was obtained under the optimal extraction conditions as follows: extraction temperature 89 °C, extraction time 3h, ratio of water volume (mL) to raw material weight (g) 29 (v/w), and extraction number two times. The present research not only provide theoretical basis for exploitation of natural polysaccharide antioxidants, but also establish the foundation of large-scale production and further system utilization of ZBP.

  15. Extraction optimization, characterization and immunity activity of polysaccharides from Fructus Jujubae.

    Science.gov (United States)

    Cui, Guoting; Zhang, Wuxia; Wang, Qingjie; Zhang, Amin; Mu, Haibo; Bai, Hongjin; Duan, Jinyou

    2014-10-13

    The versatile Fructus Jujubae is widely used in Chinese and Korean traditional medicine. In this study, the extraction optimization, characterization and immunostimulatory activities of polysaccharides from Fructus Jujubae were investigated. Based on a four-variable-three-level Box-Behnken statistical design, the optimal extraction parameters were optimized as follows: extraction temperature 90 °C, extraction time 3.23 h, water to raw material ratio 33:1 and extraction 3 times. Under these conditions, the experimental yield of polysaccharides was 6.47 ± 0.26%, which was close to the predicted yield value (6.54%). The crude Fructus Jujubae polysaccharide was further purified by DEAE-cellulose chromatography repeatedly, and two homogenous fractions, designated as RQP1d and RQP2d with molecular weight of 83.8 and 123.0 kDa respectively, were obtained. Their structures were determined by chemical analysis, Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). Finally, preliminary immunological tests indicated that both RQP1d and RQP2d significantly stimulated NO production in RAW264.7 macrophages, and promoted LPS-induced splenocyte proliferation. These data implied Fructus Jujubae polysaccharides had the potential to be explored as novel natural immunostimulant for using in functional foods or medicine.

  16. The Antioxidative, Antiaging, and Hepatoprotective Effects of Alkali-Extractable Polysaccharides by Agaricus bisporus

    Directory of Open Access Journals (Sweden)

    Shangshang Li

    2017-01-01

    Full Text Available The aim of this work was designed to investigate the antioxidant, antiaging, and hepatoprotective effects of alkali-extractable polysaccharides (AlAPS and their three purified fractions (AlAPS-1, AlAPS-2, and AlAPS-3 from Agaricus bisporus in D-galactose induced aging mice. For in vitro antioxidant analysis, both AlAPS and its fractions exhibited moderate reducing power, Fe2+-chelating activities, and potent scavenging activities on hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH radicals. The in vivo results demonstrated that the polysaccharides, especially AlAPS-2, showed potential antiaging and hepatoprotective effects by enhancing the antioxidant status, decreasing serum hepatic enzyme activities, and improving the lipid metabolism. This study suggested that the polysaccharides extracted and purified from A. bisporus could be exploited as a potent dietary supplement to attenuate aging and prevent age-related diseases.

  17. Extraction, partial characterization and bioactivity of polysaccharides from boat-fruited sterculia seeds.

    Science.gov (United States)

    Ai, Lianzhong; Wu, Jinhong; Che, Na; Wu, Yan; Cui, Steve W

    2012-12-01

    Three polysaccharides (water-soluble (WSP), alkali-soluble (ASP) and insoluble (IMP)) from boat-fruited sterculia seeds were obtained using different extraction methods. Moisture, ash, protein and total carbohydrate content of WSP, ASP and IMP were analyzed. WSP was rich in glucose, rhamnose, arabinose and galactose while small amount of xylose was also detected. The monosaccharide composition as well its relative content for WSP and ASP were similar. The intrinsic viscosity results demonstrated that ASP had much lower intrinsic viscosity than WSP, indicating partial polysaccharides were degraded into low molecular weight polymers during alkaline extraction. The acute anti-inflammatory bioactive results of polysaccharides indicated that WSP demonstrated an inhibitive effect toward acute inflammation.

  18. Optimization of microwave-assisted extraction of polysaccharide from Psidium guajava L. fruits.

    Science.gov (United States)

    Amutha Gnana Arasi, Michael Antony Samy; Gopal Rao, Manchineela; Bagyalakshmi, Janardanan

    2016-10-01

    This study deals with the optimization of microwave assisted extraction of polysaccharide from Psidium guajava L. fruit using Response surface methodology. To evaluate the effect of three independent variables, Water to plant material ratio, microwave power used for extraction and Irradiation time, central composite design has been employed. The yield is considered as dependent variable. The design model estimated the optimum yield of 6.81677% at 200W microwave power level, 3:1 water to plant material ratio and 20min of irradiation time. Three factors three levels Central composite design coupled with RSM was used to model the extraction process. ANOVA was performed to find the significance of the model. The polysaccharide extracted using microwave assisted extraction process was analyzed using FTIR Spectroscopy.

  19. Antioxidant property of water-soluble polysaccharides from Poria cocos Wolf using different extraction methods.

    Science.gov (United States)

    Wang, Nani; Zhang, Yang; Wang, Xuping; Huang, Xiaowen; Fei, Ying; Yu, Yong; Shou, Dan

    2016-02-01

    Poria cocos Wolf is a popular traditional medicinal plant that has invigorating activity. Water-soluble polysaccharides (PCPs) are its main active components. In this study, four different methods were used to extract PCPs, which include hot water extraction (PCP-H), ultrasonic-assisted extraction (PCP-U), enzyme-assisted extraction (PCP-E) and microwave-assisted extraction (PCP-M). Their chemical compositions and structure characterizations were compared. In vitro antioxidant activities were studied on the basis of DPPH radical, hydroxyl radical, reducing power and metal chelating ability. The results showed that PCPs were composed of mannose, glucose, galactose, and arabinose, and had typical IR spectra characteristics of polysaccharides. Compared with other PCPs, PCP-M had lower neutral sugar content, higher mannose content and higher uronic acid content. The molecular weight were determined as PCP-Ecocos Wolf.

  20. Structural investigation of water-soluble polysaccharides extracted from the fruit bodies of Coprinus comatus

    NARCIS (Netherlands)

    Li, Bo; Dobruchowska, Justyna M.; Gerwig, Gerrit J.; Dijkhuizen, Lubbert; Kamerling, Johannis P.

    2013-01-01

    Water-soluble polysaccharide material, extracted from the stipes of the fruit bodies of Coprinus comatus by hot water, was fractionated by sequential weak anion-exchange and size-exclusion chromatography. The relevant fractions were subjected to structural analysis, including (D/L) monosaccharide/me

  1. Designed optimization of a single-step extraction of fucose-containing sulfated polysaccharides from Sargassum sp

    DEFF Research Database (Denmark)

    Ale, Marcel Tutor; Mikkelsen, Jørn Dalgaard; Meyer, Anne S.

    2012-01-01

    Fucose-containing sulfated polysaccharides can be extracted from the brown seaweed, Sargassum sp. It has been reported that fucose-rich sulfated polysaccharides from brown seaweeds exert different beneficial biological activities including anti-inflammatory, anticoagulant, and anti-viral effects....... Classical extraction of fucose-containing sulfated polysaccharides from brown seaweed species typically involves extended, multiple-step, hot acid, or CaCl2 treatments, each step lasting several hours. In this work, we systematically examined the influence of acid concentration (HCl), time, and temperature...... on the yield of fucosecontaining sulfated polysaccharides (FCSPs) in statistically designed two-step and single-step multifactorial extraction experiments. All extraction factors had significant effects on the fucose-containing sulfated polysaccharides yield, with the temperature and time exerting positive...

  2. Inhibitory effects of polysaccharide extract from Spirulina platensis on corneal neovascularization

    OpenAIRE

    Yang, LingLing; Wang, Yao; Zhou, Qingjun; Chen, Peng; Wang, Yiqiang; Wang, Ye; Liu, Ting; Xie, Lixin

    2009-01-01

    Purpose To assess the effects of polysaccharide extract from Spirulina platensis (PSP) on corneal neovascularization (CNV) in vivo and in vitro. Methods PSP was extracted from dry powder of Spirulina platensis. Its anti-angiogenic activity was evaluated in the mouse corneal alkali burn model after topical administration of PSP four times daily for up to seven days. Corneal samples were processed for histochemical, immunohistochemical, and gene expression analyses. The effects of PSP on prolif...

  3. Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities

    Science.gov (United States)

    Wan, Peng; Yang, Xiaoman; Cai, Bingna; Chen, Hua; Sun, Huili; Chen, Deke; Pan, Jianyu

    2015-08-01

    In the present study, ultrasonic extraction technique (UET) is used to improve the yield of polysaccharides from Laminaria japonica (LJPs). And their antioxidative as well as glycosidase inhibitory activities are investigated. Box-Behnken design (BBD) combined with response surface methodology (RSM) is applied to optimize ultrasonic extraction for polysaccharides. The optimized conditions are obtained as extraction time at 54 min, ultrasonic power at 1050 W, extraction temperature at 80°C and ratio of material to solvent at 1:50 (g mL-1). Under these optimal ultrasonic extraction conditions, an actual experimental yield (5.75% ± 0.3%) is close to the predicted result (5.67%) with no significant difference ( P > 0.05). Vitro antioxidative and glycosidase inhibitory activities tests indicate that the crude polysaccharides (LJP) and two major ethanol precipitated fractions (LJP1 and LJP2) are in a concentration-dependent manner. LJP2 (30%-60% ethanol precipitated polysaccharides) possesses the strongest α-glucosidase inhibitory activity and moderate scavenging activity against hydroxyl radicals (66.09% ± 2.19%, 3.0 mg mL-1). Also, the inhibitory activity against α-glucosidase (59.08% ± 3.79%, 5.0 mg mL-1) is close to that of acarbose (63.99% ± 3.27%, 5.0 mg mL-1). LJP1 (30% ethanol precipitated polysaccharides) exhibits the strongest scavenging activity against hydroxyl radicals (99.80% ± 0.00%, 3.0 mg mL-1) and moderate α-glucosidase inhibitory activity (47.76% ± 1.92%, 5.0 mg mL-1). LJP shows the most remarkable DPPH scavenging activity (66.20% ± 0.11%, 5.0 mg mL-1) but weakest α-glucosidase inhibitory activity (37.77% ± 1.30%, 5.0 mg mL-1). However, all these LJPs exert weak inhibitory effects against α-amylase. These results show that UET is an effective method for extracting bioactive polysaccharides from seaweed materials. LJP1 and LJP2 can be developed as a potential ingredient in hypoglycemic agents or functional food for the management of

  4. Modeling and optimization of ultrasound-assisted extraction of polysaccharide from Cucurbita moschata.

    Science.gov (United States)

    Prakash Maran, J; Mekala, V; Manikandan, S

    2013-02-15

    Polysaccharides from pumpkin were extracted by ultrasound-assisted extraction technology using four factors at five levels central composite rotatable response surface design (CCRD). On using single factor analysis, process variables such as extraction temperature (50-70 °C), power of ultrasound (50-70 W), time (15-25 min) and solid-liquid ratio (1:10-1:20 g/ml) were selected. Experiments were conducted to evaluate the effects of four independent variables on the maximum extraction yield of polysaccharides. From the experimental data, second order polynomial mathematical model were developed with high coefficient of determination values (R(2)>0.96). From response surface plots, temperature and ultrasound power exhibited independent and interactive effects on the extraction yields. Extraction temperature of 70 °C, ultrasound power of 70 W, time of 23 min and solid-liquid ratio of 1:10 g/ml were determined as optimal conditions with a maximum polysaccharides yield of 16.21%, which was confirmed through the validation of the experiments.

  5. Pulsed counter-current ultrasound-assisted extraction and characterization of polysaccharides from Boletus edulis.

    Science.gov (United States)

    You, Qinghong; Yin, Xiulian; Ji, Chaowen

    2014-01-30

    Four methods for extracting polysaccharides from Boletus edulis, namely, hot-water extraction, ultrasonic clearer extraction, static probe ultrasonic extraction, and pulsed counter-current probe ultrasonic extraction (CCPUE), were studied. Results showed that CCPUE has the highest extraction efficiency among the methods studied. Under optimal CCPUE conditions, a B. edulis polysaccharide (BEP) yield of 8.21% was obtained. Three purified fractions, BEP-I, BEP-II, and BEP-III, were obtained through sequential purification by DEAE-52 and Sephadex G-75 chromatography. The average molecular weights of BEP-I, BEP-II, and BEP-III were 10,278, 23,761, and 42,736 Da, respectively. The polysaccharides were mainly composed of xylose, mannose, galactose, and glucose; of these, mannose contents were the highest. The antioxidant activities of the BEPs were further investigated by measurement of their ability to scavenge DPPH and hydroxyl radicals as well as their reducing power. The results indicated that the BEPs have good antioxidant activity.

  6. Optimization of polysaccharides extraction from Tricholoma mongolicum Imai and their antioxidant and antiproliferative activities.

    Science.gov (United States)

    Wang, Jin; Zhao, Yongming; Li, Wei; Wang, Zhibao; Shen, Lixia

    2015-10-20

    Response surface methodology was used to optimize the enzyme-assisted extraction parameters for polysaccharides from Tricholoma mongolicum Imai (TMIPs). The orthogonal test design was employed to determine the optimal concentration of three kinds of enzyme (trypsin, pectinase and papain) and the optimal concentrations of trypsin, pectinase and papain were 1.5%, 2.5%, and 2.0% (dry weight % of T. mongolicum Imai powder), respectively. In addition, three variables that remarkably affected the yield of polysaccharides such as extraction temperature, pH and extraction time were studied based on a Box-Behnken design. The results demonstrated that extraction time was the most remarkable factor affecting the TMIPs yield, followed by pH and temperature. Optimal extraction was obtained at 48.4°C, pH 5.4, and extraction time of 132min. Under these optimum conditions, the yield was 24.01%, which is consistent with the predicted value. Furthermore, crude polysaccharides were purified to obtain four fractions. In vitro antioxidant and antiproliferative activities results showed that TMIP-4 had stronger antioxidant and antiproliferative capacity than other fractions.

  7. Response surface optimization of enzyme-assisted extraction polysaccharides from Dictyophora indusiata.

    Science.gov (United States)

    Wu, Songhai; Gong, Guili; Wang, Yanyan; Li, Feng; Jia, Shaoyi; Qin, Fengxiang; Ren, Haitao; Liu, Yong

    2013-10-01

    An enzyme-assisted procedure for the extraction of the water-soluble polysaccharides from the stipe of Dictyophora indusiata was investigated using response surface methodology. The orthogonal array design was employed to optimize the concentration of three kinds of enzyme (cellulase, papain and pectolyase) and the optimal cellulose, papain and pectolyase concentration were 2.0% (wt.% of D. indusiata powder), 2.0% and 1.5%, respectively. And then the effect such as temperature, time and pH was studied based on a three-level three-factor Box-Behnken design. The optimized conditions were as follows: extraction temperature 52.5 °C, extraction time 105 min and pH 5.25. Under these conditions, the experimental yield of polysaccharides was 9.77±0.18%, which was well matched with the predictive yield of 9.87%. As it turned out, enzyme-assisted procedure was an effective method.

  8. Extraction Optimization, Purification and Physicochemical Properties of Polysaccharides from Gynura medica

    Directory of Open Access Journals (Sweden)

    Fengwei Li

    2016-03-01

    Full Text Available Extraction of polysaccharides from Gynura medica (GMPs was optimized by response surface methodology (RSM. A central composition design including three parameters, namely extraction temperature (X1, ratio of water to raw material (X2 and extraction time (X3, was used. The best conditions were extraction temperature of 91.7 °C, extraction time of 4.06 h and ratio of water to raw material of 29.1 mL/g. Under the optimized conditions, the yield of GMPs was 5.56%, which was similar to the predicted polysaccharides yield of 5.66%. A fraction named GMP-1 was obtained after isolation and purification by DEAE-52 and Sephadex G-100 gel chromatography, respectively. GMP-1, with a molecular weight of 401 kDa, mainly consisted of galacturonic acid (GalA, xylose (Xyl, glucose (Glu. Infrared spectroscopy was used to characterize the major functional groups of GMP-1 and the results indicated that it was an acidic polysaccharide. The antioxidant and α-glucosidase inhibitory activities of GMPs and GMP-1 were determined in vitro. The results indicated that GMPs and GMP-1 show potential for use in functional foods or medicines.

  9. Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purifiaction

    NARCIS (Netherlands)

    Kozarski, M.; Klaus, A.; Jakovljevic, D.; Todorovic, N.; Niksic, M.; Vrvic, M.M.; Griensven, van L.J.L.D.

    2014-01-01

    Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 ¿ 6)-ß-D-glucans. PPE was free

  10. Screening of antimicrobial potential of polysaccharide from cuttlebone and methanolic extract from body tissue of Sepia prashadi Winkworth, 1936

    Institute of Scientific and Technical Information of China (English)

    Pasiyappazham Ramasamy; Aruldhason Barwin Vino; Ramachandran Saravanan; Namasivayam Subhapradha; Vairamani Shanmugam; Annaian Shanmugam

    2011-01-01

    Objective: To evaluate the antimicrobial activity of polysaccharide from cuttlebone and methanolic extract from body tissue of Sepia prashadi, against ten human pathogenic bacteria and five fungi. Methods:The activity of polysaccharide and methanolic extract was investigated against Vibrio cholerae, Pseudomonas aeruginosa, Klebsiella pneumoniae, Vibrio alginolyticus, Staphylococcus aureus, Vibrio parahaemolyticus, Streptococcus sp., Streptococcus pneumoniae, Salmonella sp. and Escherichia coli, and five fungal strains such as Alternaria alternata, Candida tropicalis, Penicillium italicum, Fusarium equiseti and Candida albican using disc diffusion method and minimum inhibitory concentration (MIC) were also calculated. Results:Both polysaccharide and methanolic extract was active against gram positive than that of gram negative pathogenic bacteria but inactive against fungi. The MIC of both the extract ranging from 60 to 100 mg/mL. Conclusions: These results suggest that cephalopod polysaccharide and methanolic extract possess relatively good antibacterial activity.

  11. Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.

    Science.gov (United States)

    Wang, Zhen-Bin; Pei, Juan-Juan; Ma, Hai-Le; Cai, Pan-Fu; Yan, Jing-Kun

    2014-08-30

    Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively. PL-N mainly comprised xylose and arabinose with a high molecular weight (Mw) and the highest carbohydrate and uronic acid contents. PL-W and PL-A were mainly composed of glucose with high and low Mw fractions in various ratios. All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner. The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities. The Mws, monosaccharide compositions, and chemical structures of the polysaccharides also affected their antioxidant activities. PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.

  12. Extraction and characterization of polysaccharides from Semen Cassiae by microwave-assisted aqueous two-phase extraction coupled with spectroscopy and HPLC.

    Science.gov (United States)

    Chen, Zhi; Zhang, Wei; Tang, Xunyou; Fan, Huajun; Xie, Xiujuan; Wan, Qiang; Wu, Xuehao; Tang, James Z

    2016-06-25

    A novel and rapid method for simultaneous extraction and separation of the different polysaccharides from Semen Cassiae (SC) was developed by microwave-assisted aqueous two-phase extraction (MAATPE) in a one-step procedure. Using ethanol/ammonium sulfate system as a multiphase solvent, the effects of MAATPE on the extraction of polysaccharides from SC such as the composition of the ATPS, extraction time, temperature and solvent-to-material ratio were investigated by UV-vis analysis. Under the optimum conditions, the yields of polysaccharides were 4.49% for the top phase, 8.80% for the bottom phase and 13.29% for total polysaccharides, respectively. Compared with heating solvent extraction and ultrasonic assisted extraction, MAATPE exhibited the higher extraction yields in shorter time. Fourier-transform infrared spectra showed that two polysaccharides extracted from SC to the top and bottom phases by MAATPE were different from each other in their chemical structures. Through acid hydrolysis and PMP derivatization prior to HPLC, analytical results by indicated that a polysaccharide of the top phases was a relatively homogeneous homepolysaccharide composed of dominant gucose glucose while that of the bottom phase was a water-soluble heteropolysaccharide with multiple components of glucose, xylose, arabinose, galactose, mannose and glucuronic acid. Molar ratios of monosaccharides were 95.13:4.27:0.60 of glucose: arabinose: galactose for the polysaccharide from the top phase and 62.96:14.07:6.67: 6.67:5.19:4.44 of glucose: xylose: arabinose: galactose: mannose: glucuronic acid for that from the bottom phase, respectively. The mechanism for MAATPE process was also discussed in detail. MAATPE with the aid of microwave and the selectivity of the ATPS not only improved yields of the extraction, but also obtained a variety of polysaccharides. Hence, it was proved as a green, efficient and promising alternative to simultaneous extraction of polysaccharides from SC.

  13. Optimization for ultrasound-assisted extraction of polysaccharides with chemical composition and antioxidant activity from the Artemisia sphaerocephala Krasch seeds.

    Science.gov (United States)

    Zheng, Quan; Ren, Daoyuan; Yang, Nana; Yang, Xingbin

    2016-10-01

    Artemisia sphaerocephala Krasch seeds polysaccharides have been reported to have a variety of important biological activities. However, effective extraction of Artemisia sphaerocephala Krasch seeds polysaccharides is still an unsolved issue. In this study, the orthogonal rotatable central composite design was employed to optimize ultrasound-assisted extraction conditions of Artemisia sphaerocephala Krasch seeds polysaccharides. Based on a single-factor analysis method, ultrasonic power, extraction time, solid-liquid ratio and extraction temperature were shown to significantly affect the yield of polysaccharides extracted from the A. sphaerocephala Krasch seeds. The optimal conditions for extraction of Artemisia sphaerocephala Krasch seeds polysaccharides were determined as following: ultrasonic power 243W, extraction time 125min, solid-liquid ratio 64:1 and extraction temperature 64°C, where the experimental yield was 14.78%, which was well matched with the predicted value of 14.81%. Furthermore, ASKP was identified as a typical heteropolysaccharide with d-galacturonic acid (38.8%) d-galactose (20.2%) and d-xylose (15.5%) being the main constitutive monosaccharides. Moreover, Artemisia sphaerocephala Krasch seeds polysaccharides exhibited high total reducing power and considerable scavenging activities on DPPH, hydroxyl and superoxide radicals, in a concentration-dependent manner in vitro.

  14. Optimization of extraction and antioxidant activity of polysaccharides from Salvia miltiorrhiza Bunge residue.

    Science.gov (United States)

    Jiang, Yuanyuan; Wang, Long; Zhang, Li; Wang, Tao; Zhou, Yonghong; Ding, Chunbang; Yang, Ruiwu; Wang, Xiaoli; Yu, Lin

    2015-08-01

    In this study, the process of extracting polysaccharides from Salvia miltiorrhiza Bunge residue was optimized by using a Box-Behnken design. Statistical analysis of the results showed that the linear and quadratic terms of the three variables of the extraction process had significant effects. The optimal conditions are as follows: extracting time of 2.6 h, extraction temperature of 89 °C, and ratio of water to raw material of 32 mL/g. Moreover, a new polysaccharide with antioxidant activity [i.e., SMWP-1 (∼5.27×10(5) Da)] was isolated from S. miltiorrhiza residue. The carbohydrate, uronic acid, and protein contents of SMWP-1 were 90.11%, 0.13%, and 0.53%, respectively. The SMWP-1 is composed of glucose, xylose, mannose, and galactose. The preliminary structural characterization of SMWP-1 was determined via Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) analyses. This polysaccharide exhibited strong reducing power and free-radical scavenging activities in vitro against 2,2-diphenyl-1-picrylhydrazyl, superoxide anion, and hydroxyl. Therefore, SMWP-1 can be investigated further as a novel natural antioxidant.

  15. Ultrasonic-enchanced compound-enzymes-assisted extraction of polysaccharides from Cornus officinalis

    Directory of Open Access Journals (Sweden)

    You Qinghong

    2015-01-01

    Full Text Available Process of ultrasonic-enchanced compound-enzymes-assisted extraction of polysaccharides from the fruit of Cornus officinalis was optimized by response surface methodology (RSM. The influence of three different factors on the yield of Cornus officinalis polysaccharides (COP was studied. Results showed that the optimal conditions were extraction temperature 49.6°C, ultrasound time 40.41 min, and ultrasound power 308.07 W. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and were analyzed by analysis of variance (ANOVA. The 3-D response surface plot and the contour plot derived from the mathematical models were applied to determine the optimal conditions. Under modificated conditions, the experimental yield of polysaccharides was 11.02 ± 0.41%, which was well in agreement with the value predicted by the model. A good 1,1-diphenyl-2-picryldydrazyl (DPPH radical scavenging activity of COP extracted by ultrasonic-enchanced compound-enzymes-assisted extraction was observed.

  16. Novel functional polysaccharides from Radix Polygoni Multiflori water extracted residue: Preliminary characterization and immunomodulatory activity.

    Science.gov (United States)

    Zhang, Qing; Xu, Yi; Zou, Sheng; Zhang, Xiaodan; Cao, Kun; Fan, Qi

    2016-02-10

    The alkali-extractable polysaccharides (APMPs) were isolated from the water extracted residues of Radix Polygoni Multiflori, and further purified by DEAE-52 cellulose and Sephadex G-100 column chromatography to obtain a homogeneous polysaccharide (APMP-2) with molecular weights of 7724.8 Da. HPLC chromatography analysis identified that APMP-2 was a heteropolysaccharides and mainly composed of Galactose and Xylose with a molar ratio of 4.31: 1.06. It was shown that both APMP and APMP-2 were of activation effects on splenocytes and peritoneal macrophages, and also significantly restore the proliferation rate, phagocytic index and cytokine (IL-2 and TNF-α) production level of 5-FU-treated splenocytes/peritoneal macrophages in a dosage-dependent manner. The results suggested that polysaccharides presented in Radix Polygoni Multiflori water-extracted residues possessed immunomodulatory activity and could be used as potential immunomodulators, and this finding could be a reference for the utilization of Radix Polygoni Multiflori water extracted residues.

  17. Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea Cakes

    Directory of Open Access Journals (Sweden)

    Hongzhi Liu

    2016-06-01

    Full Text Available The hot-water extraction, purification and characterization of polysaccharides from defatted peanut cake (PPC were investigated in this study. A Box-Behnken factorial design (BBD was used to investigate the effects of three independent variables, namely extraction temperature (X1, extraction time (X2 and ratio of water to raw material (X3. The optimum conditions were 85 °C, 3 h and 20:1 (mL/g respectively. Regression analysis was done to reveal the experimental results which include 34.97% extraction rate while the value verified under these conditions was 34.49%. The crude PPC was sequentially further purified by Sephadex G-100 chromatography, and one purified fraction was obtained. The PPC purified fraction was characterized by FT-IR, HPAEC; SEC-MALLS. The average molecular weight of the PPC purified fraction was 2.383 × 105 Da. The polysaccharide was mainly composed of glucose, galactose, arabinose and xylose. The PPC have the typical absorption of polysaccharide.

  18. Extraction, characterization and antioxidant activities of polysaccharides from E. corneum gigeriae galli.

    Science.gov (United States)

    Xiong, Qingping; Li, Xia; Zhou, Ruizhen; Hao, Hairong; Li, Songlin; Jing, Yi; Zhu, Chun; Zhang, Qinghua; Shi, Yingying

    2014-08-08

    In the present study, optimization of enzyme-assisted extraction, characterization and antioxidant activities in vitro of polysaccharides from Endothelium corneum gigeriae galli (PEGG) were investigated. It was found that the optimum extraction conditions were determined as follows: extraction temperature 87.0°C, extraction time 177.0 min, enzyme concentration 1.65%, enzymatic hydrolysis time 141.0 min, liquid-to-solid ratio 20, enzymatic hydrolysis temperature 55°C and enzymatic hydrolysis pH 3.6. Under these conditions, the experimental yield of polysaccharides was 5.08%. In addition, PEGG had a relatively high sulfate radical content. PEGG was composed of rhamnose, fucose, mannose, glucose and galactose, with molar percentages of 13.1, 4.5, 72.8 and 9.6%, respectively. The average molecular weight was 83 kDa. And there were infrared characteristic absorption peaks of polysaccharides in the FT-IR spectroscopy of PEGG. For antioxidant activities in vitro, PEGG showed possessed strong hydroxyl radical scavenging, Fe(2+) chelating and lipid peroxidation inhibitory activities.

  19. Fucose-containing sulfated polysaccharides from brown seaweed: Extraction technolgy and bioactivity assessment

    DEFF Research Database (Denmark)

    Ale, Marcel Tutor

    seaweeds, and investigate their bioactivity. To this effect, designed optimized extraction of fucose-containing sulfated polysaccharides (FCSPs) and/or crude fucoidan from brown seaweed were performed, and the bioactivity of the isolated FCSPs was investigated. Moreover, to assess the potential of seaweed...... to assimilate nitrogen-based nutrients, a technology for accurate monitoring of differential seaweed growth responses to nutrient assimilation was also developed. Fucoidan is a term used to describe a class of sulfated polysaccharides extracted from brown seaweed, which contains substantial amounts of fucose......; varying amounts of galactose, xylose, and glucuronic acid; and differing glycosidic linkages, and are variously substituted with sulfate and acetyl groups and side branches containing fucose or other glycosyl units. These FCSPs principally consist of a backbone of (1→3)- and/or (1→4)-linked α...

  20. Cellulase-assisted extraction and antibacterial activity of polysaccharides from the dandelion Taraxacum officinale.

    Science.gov (United States)

    Wang, Hong-Bin

    2014-03-15

    In the present study, we investigated the cellulase-assisted extraction and antibacterial activity of water-soluble polysaccharides from the dandelion Taraxacum officinale. The extraction conditions, optimized for improving yield, were as follows: time, 46.11 min; temperature, 54.87 °C; pH, 4.51 and cellulase enzyme, 4000 U/g. Under these conditions, the yield of polysaccharides from dandelion (PD) reached 20.67% (w/w). The sugar content of PD was 95.6% (w/w), and it displayed high antibacterial activity at a concentration of 100mg/mL against Escherichia coli, Bacillus subtilis and Staphylococcus aureus. These results indicate that PD may be a viable option for use as a food preservative.

  1. Protective effect of Phellinus linteus polysaccharide extracts against thioacetamide-induced liver fibrosis in rats: a proteomics analysis

    NARCIS (Netherlands)

    Wang, H.; Wu, G.; Park, H.J.; Jiang, P.P.; Sit, W.H.; Griensven, van L.J.L.D.; Wan, J.M.F.

    2012-01-01

    Background: The hepatoprotective potential of Phellinus linteus polysaccharide (PLP) extracts has been described. However, the molecular mechanism of PLP for the inhibition of liver fibrosis is unclear. This study aims to investigate the molecular protein signatures involved in the hepatoprotective

  2. Protective effect of Phellinus linteus polysaccharide extracts against thioacetamide-induced liver fibrosis in rats: a proteomics analysis

    NARCIS (Netherlands)

    Wang, H.; Wu, G.; Park, H.J.; Jiang, P.P.; Sit, W.H.; Griensven, van L.J.L.D.; Wan, J.M.F.

    2012-01-01

    Background: The hepatoprotective potential of Phellinus linteus polysaccharide (PLP) extracts has been described. However, the molecular mechanism of PLP for the inhibition of liver fibrosis is unclear. This study aims to investigate the molecular protein signatures involved in the hepatoprotective

  3. Safety Evaluation of Turmeric Polysaccharide Extract: Assessment of Mutagenicity and Acute Oral Toxicity

    OpenAIRE

    Chandrasekaran Chinampudur Velusami; Srinivasa Rao Boddapati; Srikanth Hongasandra Srinivasa; Edwin Jothie Richard; Joshua Allan Joseph; Murali Balasubramanian; Amit Agarwal

    2013-01-01

    Curcuma longa Linn. (Zingiberaceae) commonly known as turmeric has long been used for centuries as a spice and household remedy. The present study was carried out to assess the possible mutagenic potential and acute oral toxicity of polysaccharide extract of turmeric rhizome (NR-INF-02) using standard tests. The standard battery of in vitro genotoxicity tests, bacterial reverse mutation test (BRMT), chromosome aberration (CA), and micronucleus (MN) tests were employed to assess the possible m...

  4. Extraction optimization, characterization and antioxidant activity of polysaccharide from Gentiana scabra bge.

    Science.gov (United States)

    Cheng, Zhenyu; Zhang, Yuewei; Song, Haiyan; Zhou, Hongli; Zhong, Fangli; Hu, Haobin; Feng, Yu

    2016-12-01

    In this study, optimization of smashing tissue extraction (STE), preliminary chemical characterization and antioxidant activity in vitro of crude polysaccharides (CPS) from Gentiana scabra bge (G. scabra) were investigated. The optimal extraction conditions were determined as follows: sample particle size of 80mesh, solid/liquid ratio of 1:34, extraction voltage of 157.09V and extraction time of 130.38s. Under these conditions, the extraction yield of CPS had reached 15.03±0.14% (n=3). Chemical composition analysis indicated CPS was mainly composed of mannose, rhamnose, galacturonic acid, glcose, galactose, arabinose and fucose in a molar ratio of 1.00:9.89:51.59:35.37:38.06:99.13:21.34, respectively. The average molecular weight of CPS was estimated to be 3.8×10(4)Da. In addition, the potential antioxidant activity of CPS extracted by STE were demonstrated by DPPH radical scavenging assay, superoxide anion radical scavenging assay, hydroxyl radical scavenging assay and ferric reducing power assay. Overall, this study provided an effective extraction technique for G. scabra polysaccharides which would be explored as a promising natural antioxidant agent applied in functional foods or medicines.

  5. Anti-tumor activity of polysaccharides extracted from Senecio ...

    African Journals Online (AJOL)

    carcinoma. Chunqing Dou*, Bao Zhang, Mingming Han, Xin Jin, Liyuan Sun and Tao Li. Department ... time, 3.06 h; number of extractions, 2; and ratio of water to raw material, 16.17 mL/g. ..... Yang J, Li X, Xue Y, Wang N, Liu W. Anti-hepatoma.

  6. Optimisation of high-pressure ultrasonic-assisted extraction and antioxidant capacity of polysaccharides from the rhizome of Ligusticum chuanxiong.

    Science.gov (United States)

    Liu, Jin-Liang; Zheng, Shun-Lin; Fan, Qiao-Jia; Yuan, Ji-Chao; Yang, Shi-Min; Kong, Fan-Lei

    2015-05-01

    High-pressure ultrasound-assisted extraction was used to extract polysaccharides from the Ligusticum chuanxiong rhizomes. Three independent variables, namely, extraction temperature (X1), ultrasonic power (X2) and extraction time (X3) were investigated. Response surface methodology was performed based on the results of single-factor tests. Experimental data were fitted to a second-order polynomial equation using multiple regression analysis, and results were examined using appropriate statistical methods. The optimal conditions were as follows: extraction temperature of 85°C, ultrasonic power of 187 W and extraction time of 29 min. Under these conditions, the experimental yield of polysaccharides was 5.33%, which is close to the predicted yield of 5.41%. The extracted and purified polysaccharides showed excellent antioxidative effects on 2,2-diphenyl-1-picryl-hydrazy, hydroxyl and superoxide radicals in vitro.

  7. Extraction Optimization, Preliminary Characterization and Bioactivities in Vitro of Ligularia hodgsonii Polysaccharides

    Directory of Open Access Journals (Sweden)

    Xueping Song

    2016-05-01

    Full Text Available The optimization extraction, preliminary characterization and bioactivities of Ligularia hodgsonii polysaccharides were investigated. Based on single-factor experiments and orthogonal array test, the optimum extraction conditions were obtained as follows: extraction time 3 h, temperature 85 °C, water/raw material ratio 36. Further Sevag deproteinization and dialysis yielded the dialyzed Ligularia hodgsonii polysaccharides (DLHP, 19.2 ± 1.4 mg/g crude herb. Compositional analysis, size-exclusion chromatography connected with multi-angle laser light-scattering and refractive index (SEC-MALLS-RI, Fourier transform infrared (FT-IR and 1H nuclear magnetic resonance (NMR spectroscopy were employed for characterization of the polysaccharides. DLHP was found to have a major component with a weight-average molecular weight of 1.17 × 105 Da, mainly comprising of glucose, galactose, arabinose, mannose, rhamnose, glucuronic acid and galacturonic acid. By in vitro antioxidant activity assays, DLHP presented remarkable scavenging capacities towards 1,1-diphenyl-2-picrylhydrazyl (DPPH, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS and hydroxyl radicals, and ferrous ions chelating ability. Moreover, it exhibited appreciable anti-hyperglycemic activity as demonstrated by differential inhibition of α-glucosidase and α-amylase. The results indicated that DLHP could potentially be a resource for antioxidant and hypoglycemic agents.

  8. Extraction Optimization, Preliminary Characterization and Bioactivities in Vitro of Ligularia hodgsonii Polysaccharides.

    Science.gov (United States)

    Song, Xueping; Tang, Jun

    2016-05-21

    The optimization extraction, preliminary characterization and bioactivities of Ligularia hodgsonii polysaccharides were investigated. Based on single-factor experiments and orthogonal array test, the optimum extraction conditions were obtained as follows: extraction time 3 h, temperature 85 °C, water/raw material ratio 36. Further Sevag deproteinization and dialysis yielded the dialyzed Ligularia hodgsonii polysaccharides (DLHP, 19.2 ± 1.4 mg/g crude herb). Compositional analysis, size-exclusion chromatography connected with multi-angle laser light-scattering and refractive index (SEC-MALLS-RI), Fourier transform infrared (FT-IR) and ¹H nuclear magnetic resonance (NMR) spectroscopy were employed for characterization of the polysaccharides. DLHP was found to have a major component with a weight-average molecular weight of 1.17 × 10⁵ Da, mainly comprising of glucose, galactose, arabinose, mannose, rhamnose, glucuronic acid and galacturonic acid. By in vitro antioxidant activity assays, DLHP presented remarkable scavenging capacities towards 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl radicals, and ferrous ions chelating ability. Moreover, it exhibited appreciable anti-hyperglycemic activity as demonstrated by differential inhibition of α-glucosidase and α-amylase. The results indicated that DLHP could potentially be a resource for antioxidant and hypoglycemic agents.

  9. Characteristics and Antitumor Activity of Morchella esculenta Polysaccharide Extracted by Pulsed Electric Field

    Directory of Open Access Journals (Sweden)

    Chao Liu

    2016-06-01

    Full Text Available Polysaccharides from Morchella esculenta have been proven to be functional and helpful for humans. The purpose of this study was to investigate the chemical structure and anti-proliferating and antitumor activities of a Morchella esculenta polysaccharide (MEP extracted by pulsed electric field (PEF in submerged fermentation. The endo-polysaccharide was separated and purified by column chromatography and Gel permeation chromatography, and analyzed by gas chromatography. The MEP with an average molecular weight of 81,835 Da consisted of xylose, glucose, mannose, rhamnose and galactose at the ratio of 5.4:5.0:6.5:7.8:72.3. Structure of MEP was further analyzed by Fourier-transform infrared spectroscopy and 1H and 13C liquid-state nuclear magnetic resonance spectroscopy. Apoptosis tests proved that MEP could inhibit the proliferation and growth of human colon cancer HT-29 cells in a time- and dose-dependent manner within 48 h. This study provides more information on chemical structure of anti-proliferating polysaccharides isolated from Morchella esculenta.

  10. Characteristics and Antitumor Activity of Morchella esculenta Polysaccharide Extracted by Pulsed Electric Field.

    Science.gov (United States)

    Liu, Chao; Sun, Yonghai; Mao, Qian; Guo, Xiaolei; Li, Peng; Liu, Yang; Xu, Na

    2016-06-22

    Polysaccharides from Morchella esculenta have been proven to be functional and helpful for humans. The purpose of this study was to investigate the chemical structure and anti-proliferating and antitumor activities of a Morchella esculenta polysaccharide (MEP) extracted by pulsed electric field (PEF) in submerged fermentation. The endo-polysaccharide was separated and purified by column chromatography and Gel permeation chromatography, and analyzed by gas chromatography. The MEP with an average molecular weight of 81,835 Da consisted of xylose, glucose, mannose, rhamnose and galactose at the ratio of 5.4:5.0:6.5:7.8:72.3. Structure of MEP was further analyzed by Fourier-transform infrared spectroscopy and ¹H and (13)C liquid-state nuclear magnetic resonance spectroscopy. Apoptosis tests proved that MEP could inhibit the proliferation and growth of human colon cancer HT-29 cells in a time- and dose-dependent manner within 48 h. This study provides more information on chemical structure of anti-proliferating polysaccharides isolated from Morchella esculenta.

  11. Chemical composition and antioxidant activity of an acidic polysaccharide extracted from Cucurbita moschata Duchesne ex Poiret.

    Science.gov (United States)

    Yang, Xingbin; Zhao, Yan; Lv, You

    2007-06-13

    A simple and sensitive high-performance capillary electrophoresis (HPCE) method was designed for quantitative analysis of the component monosaccharides of an acidic polysaccharide extracted from pumpkin. In this method, the polysaccharide was hydrolyzed into component monosaccharides with 2.0 M trifluoroacetic acid at 100 degrees C for 6 h and then labeled with 1-phenyl-3-methyl-5-pyrazolone, and subsequently the labeled monosaccharide derivatives were separated by HPCE. As a result, glucose (21.7%) and glucuronic acid (18.9%) were identified to be the main component monosaccharides, followed by galactose (11.5%), arabinose (9.8%), xylose (4.4%), and rhamnose (2.8%). Furthermore, the pumpkin polysaccharide was also demonstrated to effectively inhibit the H2O2-caused decrease of cell viability, lactate dehydrogenase leakage, and malondialdehyde formation, and also reduced the H2O2-caused decline of superoxide dismutase activity and glutathione depletion in cultured mouse peritoneal macrophages, indicating that pumpkin polysaccharide possessed significant cytoprotective effect and antioxidative activity.

  12. In vitro antioxidant effects and cytotoxicity of polysaccharides extracted from Laminaria japonica.

    Science.gov (United States)

    Peng, Zhenfei; Liu, Min; Fang, Zhexiang; Zhang, Qiqing

    2012-06-01

    A water-soluble crude polysaccharide (WPS) was obtained from Laminaria japonica by hot water extraction. Three major polysaccharide fractions (WPS-1, WPS-2 and WPS-3) were purified from WPS by anion-exchange chromatography. Monosaccharide components analysis indicated that galactose was the predominant monosaccharide in WPS and WPS-3, accounting for 56.25% and 54.11%, respectively. And fucose was the predominant monosaccharide in WPS-1 and WPS-2, accounting for 46.91% and 45.1%, respectively. Antioxidant activity tests revealed that WPS-2 showed significant function of scavenging hydroxyl free radical and WPS-1 exhibited the highest inhibitory effects on superoxide radical. Cytotoxicity of all polysaccharide fractions was evaluated by MTT assay and Hoechst 33258 staining. Results showed that WPS-1 and WPS-2 significantly inhibited the growth of A375 cells and low anti-proliferative effects of WPS-2 on vascular smooth muscle cells (VSMCs) were observed. These results suggested that the polysaccharide fraction of WPS-2 might be explored as a potential safe antioxidant and antitumor agent.

  13. Characteristics and Antitumor Activity of Morchella esculenta Polysaccharide Extracted by Pulsed Electric Field

    Science.gov (United States)

    Liu, Chao; Sun, Yonghai; Mao, Qian; Guo, Xiaolei; Li, Peng; Liu, Yang; Xu, Na

    2016-01-01

    Polysaccharides from Morchella esculenta have been proven to be functional and helpful for humans. The purpose of this study was to investigate the chemical structure and anti-proliferating and antitumor activities of a Morchella esculenta polysaccharide (MEP) extracted by pulsed electric field (PEF) in submerged fermentation. The endo-polysaccharide was separated and purified by column chromatography and Gel permeation chromatography, and analyzed by gas chromatography. The MEP with an average molecular weight of 81,835 Da consisted of xylose, glucose, mannose, rhamnose and galactose at the ratio of 5.4:5.0:6.5:7.8:72.3. Structure of MEP was further analyzed by Fourier-transform infrared spectroscopy and 1H and 13C liquid-state nuclear magnetic resonance spectroscopy. Apoptosis tests proved that MEP could inhibit the proliferation and growth of human colon cancer HT-29 cells in a time- and dose-dependent manner within 48 h. This study provides more information on chemical structure of anti-proliferating polysaccharides isolated from Morchella esculenta. PMID:27338370

  14. Purification, characterization and biological activities in vitro of polysaccharides extracted from tea seeds.

    Science.gov (United States)

    Wang, Yuanfeng; Liu, Yangyang; Mao, Fangfang; Liu, Yiran; Wei, Xinlin

    2013-11-01

    Three polysaccharide fractions (NTSPS, ATSPS1-1 and ATSPS2) were isolated and purified from the water extract of tea (Camellia sinensis) seeds with yields of 32.74%, 25.22%, and 11.09%, respectively. The average molecular weight of NTSPS, ATSPS1-1 and ATSPS2 was determined by HPGPC system, with an Mw of 4.588, 500 and 100 kDa, respectively. Monosaccharide analysis by ion chromatography revealed they differed from each other in monosaccharide kinds and molar ratio. Furthermore, ATSPS1-1 and ATSPS2 were heteropolysaccharide bounded with protein (2.83% and 2.12%) and 17 general amino acids were detected in them on an ion-chromatographic analyzer. The IR spectrum of ATSPS1-1 and ATSPS2 revealed the typical characteristics of polysaccharides and protein. In addition, the antitumor and immunological activity of the three polysaccharide fractions in vitro were also evaluated. It was found inhibition activity of NTSPS, ATSPS1-1 and ATSPS2 on K562 cells increased with increasing sample concentration and the inhibition ratios of them at 400 μg/mL were beyond 30.13 ± 3.54%, 36.61 ± 2.75% and 32.33 ± 2.53%, respectively. They also exhibited strong ability in promoting proliferation of mice splenic lymphocyte. Results of these studies indicated the three purified polysaccharides had a potential application as natural antitumor drugs.

  15. Optimization of ultrasonic-assisted extraction of antioxidant polysaccharides from the stem of Trapa quadrispinosa using response surface methodology.

    Science.gov (United States)

    Raza, Aun; Li, Feng; Xu, Xiuquan; Tang, Jian

    2017-01-01

    Response surface methodology (RSM) was used to optimize experimental conditions for ultrasonic-assisted extraction (UAE) of antioxidant crude polysaccharides (CPS) from Trapa quadrispinosa stems. Antioxidant capacity of polysaccharides was determined by Ferric-Reducing Antioxidant Capacity (FRAC) assay with the method of 1, 10-phenanthroline. The maximum yield of polysaccharides (2.78±0.16%) was obtained under optimal extraction conditions of extraction time, 41min; ratio of water to material, 31.5mL/g; and extraction temperature, 58°C. The maximum antioxidant capacity (19.02±0.24μmol Fe(2+)/g) was obtained under the optimal extraction conditions of extraction time, 38min; ratio of water to material, 32mL/g; and extraction temperature, 56°C. These two values were agreed well with predicted yield (2.75%) and antioxidant capacity (18.77μmol Fe(2+)/g). Antioxidant activities of CPS were investigated by various assays. The results showed that CPSUAE obtained by UAE showed higher 1,1-Diphenyl-2-picrylhydrazxyl (DPPH), 2,2-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activities and stronger reducing power, total antioxidant capacity compared with CPSHWE obtained by hot water extraction (HWE).The results indicated that UAE is an advisable method for extraction of antioxidant polysaccharides from the stem of T. quadrispinosa and polysaccharides could be explored as potential antioxidant to use in medicine or functional food.

  16. Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata).

    Science.gov (United States)

    Wu, Hao; Zhu, Junxiang; Diao, Wenchao; Wang, Chengrong

    2014-11-26

    An efficient ultrasound-assisted enzymatic extraction (UAEE) of Cucurbita moschata polysaccharides (CMCP) was established and the CMCP antioxidant activities were studied. The UAEE operating parameters (extraction temperature, ultrasonic power, pH, and liquid-to-material ratio) were optimized using the central composite design (CCD) and the mass transfer kinetic study in UAEE procedure was used to select the optimal extraction time. Enzymolysis and ultrasonication that were simultaneously conducted was selected as the UAEE synergistic model and the optimum extraction conditions with a maximum polysaccharide yield of 4.33 ± 0.15% were as follows: extraction temperature, 51.5 °C; ultrasonic power, 440 W; pH, 5.0; liquid-to-material ratio, 5.70:1 mL/g; and extraction time, 20 min. Evaluation of the antioxidant activity in vitro suggested that CMCP has good potential as a natural antioxidant used in the food or medicine industry because of their high reducing power and positive radical scavenging activity for DPPH radical.

  17. Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry (Morus alba L.) leaves.

    Science.gov (United States)

    Yuan, Qingxia; Xie, Yufeng; Wang, Wei; Yan, Yuhua; Ye, Hong; Jabbar, Saqib; Zeng, Xiaoxiong

    2015-09-05

    Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry leaves (MLP) were investigated in the present study. The optimal extraction conditions with an extraction yield of 10.0 ± 0.5% for MLP were determined as follows: extraction temperature 92 °C, extraction time 3.5h and ratio (v/w, mL/g) of extraction solvent (water) to raw material 34. Two purified fractions, MLP-3a and MLP-3b with molecular weights of 80.99 and 3.64 kDa, respectively, were obtained from crude MLP by chromatography of DEAE-Cellulose 52 and Sephadex G-100. Fourier transform-infrared spectroscopy revealed that crude MLP, MLP-3a and MLP-3b were acidic polysaccharides. Furthermore, crude MLP and MLP-3a had more complicated monosaccharide compositions, while MLP-3b had a relatively higher content of uronic acid. Crude MLP, MLP-3a and MLP-3b exhibited potent Fe(2+) chelating power and scavenging activities on 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide and 2,2'-azinobis-(3-ethyl-benzothiazolin-6-sulfonic acid) radicals. The results suggested that MLP could be explored as natural antioxidant.

  18. Optimization of microwave-assisted enzymatic extraction of polysaccharides from the fruit of Schisandra chinensis Baill.

    Science.gov (United States)

    Cheng, Zhenyu; Song, Haiyan; Yang, Yingjie; Liu, Yan; Liu, Zhigang; Hu, Haobin; Zhang, Yang

    2015-05-01

    A microwave-assisted enzymatic extraction (MAEE) method had been developed, which was optimized by response surface methodology (RSM) and orthogonal test design, to enhance the extraction of crude polysaccharides (CPS) from the fruit of Schisandra chinensis Baill. The optimum conditions were as follows: microwave irradiation time of 10 min, extraction pH of 4.21, extraction temperature of 47.58°C, extraction time of 3h and enzyme concentration of 1.5% (wt% of S. chinensis powder) for cellulase, papain and pectinase, respectively. Under these conditions, the extraction yield of CPS was 7.38 ± 0.21%, which was well in close agreement with the value predicted by the model. The three methods including heat-refluxing extraction (HRE), ultrasonic-assisted extraction (UAE) and enzyme-assisted extraction (EAE) for extracting CPS by RSM were further compared. Results indicated MAEE method had the highest extraction yields of CPS at lower temperature. It was indicated that the proposed approach in this study was a simple and efficient technique for extraction of CPS in S. chinensis Baill.

  19. Extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp.

    Science.gov (United States)

    Du, Hongtao; Chen, Juncheng; Tian, Shan; Gu, Hongling; Li, Na; Sun, Yao; Ru, Jiajia; Wang, Junru

    2016-10-20

    In this research, extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp were investigated. A response surface methodology (RSM) with a Box-Behnken design (BBD) was used to optimize the extraction process. The maximum EAP yield was 9.82±0.38%, which is in good agreement with the predicted value (9.93±0.24%). Two homogeneous polysaccharides, EAP-1a and EAP-1b with molecular weights of 8.70kDa and 4.39kDa respectively, were prepared by DEAE-52 cellulose and Sephadex G-100 columns and characterized by HPLC, HPGPC, and FT-IR. Three polysaccharides (EAP, EAP-1a and EAP-1b) could stimulate macrophages to release NO and enhance phagocytic activities of RAW 264.7 cells in dose-dependent manner. Moreover, there was no significant difference between crude EAP group (400μg/mL) and positive control group (LPS) in effects on macrophages. The results implied that EAP had the potential to be developed as natural medicines or health foods.

  20. Influence of polysaccharides and storage during processing on the properties of mango seed kernel extract (microencapsulation).

    Science.gov (United States)

    Maisuthisakul, Pitchaon; Gordon, Michael H

    2012-10-01

    Extracts from mango (Mangifera indica Linn.) cultivar Chok-Anan seed kernels were studied as active substances, since they are known as a good source of phenolic antioxidants with metal chelating and tyrosinase inhibitory activities. Response surface methodology (RSM) was used to investigate the effect of a combination of polysaccharides selected from gum arabic, maltodextrin and alginate on droplet size distribution, encapsulation efficiency (EE), stability and viscosity of W/O/W emulsions. In addition, the effects of stored emulsion on the properties of the encapsulated powder were studied. The results showed that there were interactions between polysaccharides which affected droplet size distribution, stability, viscosity and EE of multiple emulsions. The RSM showed a good fit to the proposed model with R(2)>0.83, 0.79 and 0.69 for viscosity, stability and EE, respectively, with significant correlations (pcoating material was also a suitable coating mixture for preparation of encapsulated mango seed kernel extract powder. Moreover, if the polysaccharide combination is not appropriate for coating, the storage after emulsion preparation will have a greater effect on the properties of the encapsulated emulsion and powder.

  1. Microwave-assisted extraction of jujube polysaccharide: Optimization, purification and functional characterization.

    Science.gov (United States)

    Rostami, Hosein; Gharibzahedi, Seyed Mohammad Taghi

    2016-06-05

    The operational parameters involved in microwave-assisted extraction (MAE) of jujube polysaccharide including microwave power, water to raw material ratio and extraction temperature and time were optimized by RSM. MAE at 400W, 75°C, 60 min, using 30 g water/g powdered jujube was the best condition for maximum yield (9.02%) of polysaccharide. Two novel water-soluble polysaccharides (JCP-1 and JCP-2) with average molecular weights of 9.1×10(4)-1.5×10(5)Da in term of the symmetrical narrow peaks were identified using the analytical purification procedures. The JCP-1 and JCP-2 mainly composed of glucose, arabinose, galactose and rhamnose in molar ratios of 1.4:2.1:4.2:0.9 and 1.2:1.8:4.1:1.1, respectively. The use of 1.5% JCP-1 led to a high emulsifying stability (95.5%) in a model oil-in-water type emulsion with a reduced surface tension (44.1 mN/m) and droplet size (1.32 μm), and an increased apparent viscosity (0.13 Pas) during 21-day cold storage. The antioxidant activities were increased in dose-dependent manners (25-200 μg/mL).

  2. Optimization of Vacuum-Microwave Radiation Pretreatment on Extraction of Ganoderma Polysaccharides

    Directory of Open Access Journals (Sweden)

    C. F. Song

    2015-01-01

    Full Text Available A new process of vacuum-microwave (VM radiation pretreatment for extracting polysaccharides from the Ganoderma lucidum was proposed, and the parameters were optimized by response surface methodology (RSM. The orthogonal-central composite design scheme was used and the responsive surfaces methodology of three factors and five levels was adopted, and the factors influencing the technological parameters and its interaction terms were analyzed and regressed. The optimal parameters were obtained as follows: the infiltration time of 70 min, microwave power density of 11.2 W/g, and VM irradiation time of 180 s. In consequence, the extraction yield was up to 1.775% when VM radiation was conducted in advance. Compared to the traditional hot-water extraction method, VM pretreatment can shorten the extraction time by more than a half, and the polysaccharide extraction yield was increased by 48.1%. It holds significant potential for further investigation, development, and application.

  3. Comparison of two chemical extraction methods for proteins and polysaccharides of Spirogyra fluviatilis in extracellular polymeric substances

    Science.gov (United States)

    Chang, Shuiping; Lee, Yichao

    2017-05-01

    Under natural circumstances, Spirogyra fluviatilis excretes large amounts of extracellular polymeric substances (EPS) and forms a mucilage layer. This study used two algal EPS extraction methods such as NH4OH and formaldehyde-NaOH to compare the extraction yields of EPS and the levels of its primary components of polysaccharides and proteins. The results indicated that the extraction yields of EPS using formaldehyde-NaOH and NH4OH are 8.05 mg EPS/g-fresh algae and 5.26 mg EPS/g-fresh algae, respectively. The ratio of proteins to polysaccharides in EPS is around 0.24 to 0.34, which is not a significant difference between the two extraction methods. In other words, the level of polysaccharides is always higher than the level of proteins in EPS extraction with both two methods

  4. Ultrasound-assisted extraction of polysaccharides from Rhododendron aganniphum: Antioxidant activity and rheological properties.

    Science.gov (United States)

    Guo, Xiao; Shang, Xiaofei; Zhou, Xuzheng; Zhao, Baotang; Zhang, Jiyu

    2017-09-01

    In this study, we aimed to optimize the extraction of polysaccharides from the leaves of Rhododendron aganniphum and investigate its rheological properties and antioxidant activity. After optimizing the operating parameters using a Box-Behnken design (BBD), the results showed that the optimal ultrasound-assisted extraction conditions were as follows: extraction temperature, 55°C; liquid-solid ratio, 25:1; extraction time, 2.2h; and ultrasound treatment power, 200W. The optimized experimental yield of polysaccharides by ultrasound-assisted extraction (PUAE) was 9.428%, higher than that obtained by hot water extraction (PHWE) for 12h at the same liquid-solid ratio and extraction temperature. In the in vitro antioxidant activity tests, PUAE had higher positive radical scavenging activity for hydroxyl, superoxide and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals than PHWE. However, PUAE and PHWE solutions had similar intermolecular interactions in the steady-shear flow and dynamic viscoelasticity tests, resulting in similar macroscopic behaviour. With respect to the apparent viscosity, storage modulus (G') and loss modulus (G″) of PUAE were lower at the same shear rate or angular frequency. All PUAE solutions exhibited non-Newtonian shear-thinning pseudoplastic behaviour that was accurately described by the Carreau model but was better fit by the power-law model at high shear rates (≥1/s), which demonstrated that the variation in the apparent viscosity dependence was greater at higher concentrations and shear rates. The G' and G″ of the solutions increased as the experimental frequency increased from 0.05 to 500rad/s under all experimental concentrations, and the modulus crossover point decreased gradually with increasing PUAE concentration. The above results demonstrated that the ultrasound-assisted extraction methods gave a higher yield of polysaccharides from the leaves of R. aganniphum with a shorter extraction time than the hot water extraction method

  5. Two-steps extraction of essential oil, polysaccharides and biphenyl cyclooctene lignans from Schisandra chinensis Baill fruits.

    Science.gov (United States)

    Cheng, Zhenyu; Yang, Yingjie; Liu, Yan; Liu, Zhigang; Zhou, Hongli; Hu, Haobin

    2014-08-05

    A method for two-steps extraction of essential oil, polysaccharides and lignans from Schisandra chinensis Baill had been established. Firstly, S. chinensis was extracted by hydro-distillation, the extracted solution was separated from the water-insoluble residue and precipitated by adding dehydrated alcohol after the essential oil was collected, and then the precipitate as polysaccharide was collected. Finally, second extraction was performed to obtained lignans from the water-insoluble residue with ultrasonic-microwave assisted extraction (UMAE) method. Response surface methodology was employed to optimize the UMAE parameters, the optimal conditions were as follows: microwave power 430W, ethanol concentration 84%, particle size of sample 120-mesh sieves, ratio of water to raw material 15 and extraction time 2.1min. Under these optimized conditions, the total extraction yields of five lignans (Schisandrol A, Schisantherin A, Deoxyschisandrin, Schisandrin B and Schisandrin C) had reached 14.22±0.135mg/g. Compared with the traditional method of direct extraction of different bioactive components in respective procedure, the extraction yields of polysaccharides and the five lignans had reached 99% and 95%, respectively. The mean recoveries of the 5 lignan compounds and polysaccharides were 97.75-101.08% and their RSD value was less than 3.88%.The approach proposed in this study not only improved the extraction yield of lignans, but also elevated the utilization of Schisandra resources.

  6. Extraction, characterization and in vitro antioxidant activity of polysaccharides from black soybean.

    Science.gov (United States)

    Liu, Jun; Wen, Xiao-yuan; Zhang, Xue-qing; Pu, Hui-min; Kan, Juan; Jin, Chang-hai

    2015-01-01

    Optimization of extraction conditions, preliminary characterization and in vitro antioxidant activity of polysaccharides from black soybean (BSPS) were investigated. The results of Box-Behnken design showed that the optimal extraction conditions for BSPS were as follows: ratio of water to material of 20 ml/g, extraction time of 6.4h and extraction temperature of 92 °C, with a corresponding yield of 2.56%. The crude BSPS were further fractionated on DEAE-52 and Sepharose CL-4B chromatography to afford three purified fractions (BSPS-1, BSPS-2 and BSPS-3). Chemical analysis showed that the three purified fractions were mainly composed of carbohydrate and uronic acid. In addition, BSPS-1 was composed of arabinose, rhamnose, galactose, glucose and mannose in the molar ratio of 1.79:1.00:2.59:26.54:1.01. BSPS-2 was composed of arabinose, rhamnose, xylose, galactose and mannose in the molar ratio of 8.10:4.80:9.15:13.38:1.00. BSPS-3 was composed of arabinose, rhamnose, galactose and mannose in the molar ratio of 16.80:3.60:33.66:1.00. The results of Fourier transform-infrared spectroscopy further confirmed the characteristic polysaccharide structures of the three purified fractions. Moreover, antioxidant assays showed crude BSPS and its purified fractions had potential superoxide anion and DPPH radical scavenging activities, and their antioxidant activity decreased in the order of crude BSPS > BSPS-3 > BSPS-2 > BSPS-1.

  7. Extraction optimization of water-extracted mycelial polysaccharide from endophytic fungus Fusarium oxysporum Dzf17 by response surface methodology.

    Science.gov (United States)

    Li, Peiqin; Lu, Shiqiong; Shan, Tijiang; Mou, Yan; Li, Yan; Sun, Weibo; Zhou, Ligang

    2012-01-01

    Water-extracted mycelial polysaccharide (WPS) from the endophytic fungus Fusarium oxysporum Dzf17 isolated from Dioscorea zingiberensis was found to be an efficient elicitor to enhance diosgenin accumulation in D. zingigerensis cultures, and also demonstrated antioxidant activity. In this study, response surface methodology (RSM) was employed to optimize the extraction process of WPS from F. oxysporum Dzf17 using Box-Behnken design (BBD). The ranges of the factors investigated were 1-3 h for extraction time (X(1)), 80-100 °C for extraction temperature (X(2)), and 20-40 (v/w) for ratio of water volume (mL) to raw material weight (g) (X(3)). The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis. Statistical analysis showed that the polynomial regression model was in good agreement with the experimental results with the determination coefficient (R(2)) of 0.9978. By solving the regression equation and analyzing the response surface contour plots, the extraction parameters were optimized as 1.7 h for extraction time, 95 °C for extraction temperature, 39 (v/w) for ratio of water volume (mL) to raw material weight (g), and with 2 extractions. The maximum value (10.862%) of WPS yield was obtained when the WPS extraction process was conducted under the optimal conditions.

  8. Effects of extraction methods on the yield, chemical structure and anti-tumor activity of polysaccharides from Cordyceps gunnii mycelia.

    Science.gov (United States)

    Zhu, Zhen-Yuan; Dong, Fengying; Liu, Xiaocui; Lv, Qian; YingYang; Liu, Fei; Chen, Ling; Wang, Tiantian; Wang, Zheng; Zhang, Yongmin

    2016-04-20

    This study was to investigate the effects of different extraction methods on the yield, chemical structure and antitumor activity of polysaccharides from Cordyceps gunnii (C. gunnii) mycelia. Five extraction methods were used to extract crude polysaccharides (CPS), which include room-temperature water extraction (RWE), hot-water extraction (HWE), microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE) and cellulase-assisted extraction (CAE). Then Sephadex G-100 was used for purification of CPS. As a result, the antitumor activities of CPS and PPS on S180 cells were evaluated. Five CPS and purified polysaccharides (PPS) were obtained. The yield of CPS by microwave-assisted extraction (CPSMAE) was the highest and its anti-tumor activity was the best and its macromolecular polysaccharide (3000-1000kDa) ratio was the largest. The PPS had the same monosaccharide composition, but their obvious difference was in the antitumor activity and the physicochemical characteristics, such as intrinsic viscosity, specific rotation, scanning electron microscopy and circular dichroism spectra. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Structural elucidation and immunostimulatory activity of polysaccharide isolated by subcritical water extraction from Cordyceps militaris.

    Science.gov (United States)

    Luo, Xiaoping; Duan, Yuqing; Yang, Wenya; Zhang, Haihui; Li, Changzheng; Zhang, Jixian

    2017-02-10

    Water-soluble polysaccharides were obtained from Cordyceps militaris (C. militaris) (CMP) by subcritical water extraction (SWE). Two polysaccharides fractions, CMP-W1 and CMP-S1, were isolated from CMP using DEAE-52 cellulose and Sephadex G-150 column chromatography. The structural characteristics of CMP-W1 and CMP-S1 were investigated. The results showed that the molecular weight of CMP-W1 and CMP-S1 are 3.66×105Da and 4.60×105Da, respectively, and both of them were heteropolysaccharides composed of d-mannose, d-glucose, d-galactose with the molar ratios of 2.84:1:1.29 and 2.05:1:1.09, respectively. FT-IR spectra analysis suggested that CMP-W1 and CMP-S1 belonged to pyranose form sugar and protein free. For immunostimulatory activity assay in vitro, CMP-W1 and CMP-S1 significantly promoted lymphatic spleen cell proliferation of mice. Therefore, the polysaccharides obtained from C. militaris by SWE can be used as potential natural immunostimulant in functional foods or medicine. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Ultrasound extraction of polysaccharides from mulberry leaves and their effect on enhancing antioxidant activity.

    Science.gov (United States)

    Zhang, Dong-Yang; Wan, Yi; Xu, Jian-Yi; Wu, Guo-Hua; Li, Long; Yao, Xiao-Hui

    2016-02-10

    A Box-Behnken design (BBD) was applied to optimize the ultrasound-assisted extraction of polysaccharides from mulberry leaves. Under the optimum conditions of an extraction temperature of 57 °C, an extraction time of 80 min and a liquid/solid ratio of 53 mL/g, the mulberry leaf polysaccharide (MLP) yield was 6.92 ± 0.29%. Then, three fractions of MLPs were obtained by deproteinization, dialysis and decolorization. The carbohydrate content, FT-IR spectrum and monosaccharide composition of the MLPs were also investigated. The antioxidant activities of the three fractions were compared, and the results indicated that the antioxidant activities decreased with the increasing MLP purity. Therefore, highly concentrated MLPs were shown to have very little antioxidant activity. After quercetin (10 μg/mL) was added, the antioxidant activities were improved significantly. This result showed that MLPs and quercetin have a synergistic effect on the antioxidant activity. Although the MLPs have very little antioxidant activity alone, they greatly enhance the antioxidant activity of flavonoids. Thus, MLPs can be used as an antioxidant activity enhancer in the food industry.

  11. Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.

    Science.gov (United States)

    Zhang, Hai-Lin; Cui, Shao-Hua; Zha, Xue-Qiang; Bansal, Vibha; Xue, Lei; Li, Xiao-Long; Hao, Ran; Pan, Li-Hua; Luo, Jian-Ping

    2014-06-15

    In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP). The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h. Under these conditions, the JSP yield reached 1.007 mg/g. Papain (15 U/mL) in combination with Sevag reagent was beneficial in removing proteins from JSP. After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively. The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition. In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.

  12. Enzyme-assisted extraction of anticoagulant polysaccharide from Liparis tessellatus eggs.

    Science.gov (United States)

    Ticar, Bernadeth F; Rohmah, Zuliyati; Ambut, Carmelo V; Choi, Yeung-Joon; Mussatto, Solange I; Choi, Byeong-Dae

    2015-03-01

    This study aimed to recover a heparin-like anticoagulant polysaccharide from Liparis tessellatus eggs (PLE) by using enzyme-assisted extraction technique. Extraction experiments were carried out using three different enzymes (Alcalase®2.4 L, Flavourzyme®500 MG, and Protamex®) under different conditions of temperature (45, 50, and 55°C), pH (6.5, 7.0, and 7.5), incubation time (24, 36, and 48 h), and enzyme to substrate ratio (E/S=0.5, 1.0, and 1.5%, w/w), which were combined according to a D-optimal design. Statistical analysis of extraction results allowed identifying the variables with greater influence on the extraction yield, and selecting the conditions that maximize the PLE extraction. The best extraction results were achieved when using the Protamex® enzyme in an E/S ratio of 1.34% (w/w), pH 6.60, 47.40°C, during 26.50 h. Under these conditions, a polysaccharide yield of 2.10% (w/w) was obtained. Clotting time measurements, activated partial thromboplastin time, and prothrombin time for evaluation of the anticoagulant properties of PLE were determined and showed increasing activities in correlation with the concentrations used. In the final step, the heparin-like nature of PLE was confirmed by digestion with heparinases I, II, and III, which showed ΔDiHS-0S, ΔDiHS-6S, ΔDiHS-diS1, and ΔDiHS-diS2 at compositions of 0.04, 0.03, 0.35, and 0.24 mol/g, respectively.

  13. Extraction and Characterization of Tamarind (Tamarind indica L.) Seed Polysaccharides (TSP) from Three Difference Sources.

    Science.gov (United States)

    Chawananorasest, Khanittha; Saengtongdee, Patsuda; Kaemchantuek, Praphakorn

    2016-06-15

    Tamarind seed polysaccharide (TSP), a natural polysaccharide extracted from tamarind seeds is used in the pharmaceutical, textile and food industries as a mucoadhesive polymer. This work aimed to extract TSP from tamarind seeds from three sources with two methods and characterized its physical and chemical properties. Kernel powder of tamarind seeds was slurried into a clear solution, set aside overnight and then centrifuged at 6000 rpm for 20 min to separate all foreign matter. The supernatant was separated and poured into excess 95% ethanol with continuous stirring. The precipitate obtained was collected and dried in the oven and then the dried TSP polymer was stored in a desiccator. The dried TSP was analyzed by ¹H-NMR, FT-IR and XRD. The results showed TSP from tamarind seeds taken from paddy farmland (A), a waste from the export tamarind juice industry (B) and the export tamarind powder industry(C) gave yields of 31.55%, 26.95% and 17.30%, respectively, using method 1 and 11.15%, 53.65% and 54.65%, with method 2, respectively, but method 2 gave purer TSP than method 1. The FT-IR spectra displayed peaks at 3351.95 cm(-1), 2920.76 cm(-1), 1018.85 cm(-1) and 555.16 cm(-1). The ¹H-NMR showed polysaccharide peaks between δ 3.50-4.20 ppm and XRD diagrams indicated their amorphous nature. Future works will focus on the quantitative analysis, biological activity and possible use of TSP as a drug delivery system.

  14. Extraction and Characterization of Tamarind (Tamarind indica L. Seed Polysaccharides (TSP from Three Difference Sources

    Directory of Open Access Journals (Sweden)

    Khanittha Chawananorasest

    2016-06-01

    Full Text Available Tamarind seed polysaccharide (TSP, a natural polysaccharide extracted from tamarind seeds is used in the pharmaceutical, textile and food industries as a mucoadhesive polymer. This work aimed to extract TSP from tamarind seeds from three sources with two methods and characterized its physical and chemical properties. Kernel powder of tamarind seeds was slurried into a clear solution, set aside overnight and then centrifuged at 6000 rpm for 20 min to separate all foreign matter. The supernatant was separated and poured into excess 95% ethanol with continuous stirring. The precipitate obtained was collected and dried in the oven and then the dried TSP polymer was stored in a desiccator. The dried TSP was analyzed by 1H-NMR, FT-IR and XRD. The results showed TSP from tamarind seeds taken from paddy farmland (A, a waste from the export tamarind juice industry (B and the export tamarind powder industry(C gave yields of 31.55%, 26.95% and 17.30%, respectively, using method 1 and 11.15%, 53.65% and 54.65%, with method 2, respectively, but method 2 gave purer TSP than method 1. The FT-IR spectra displayed peaks at 3351.95 cm−1, 2920.76 cm−1, 1018.85 cm−1 and 555.16 cm−1. The 1H-NMR showed polysaccharide peaks between δ 3.50–4.20 ppm and XRD diagrams indicated their amorphous nature. Future works will focus on the quantitative analysis, biological activity and possible use of TSP as a drug delivery system.

  15. Orthogonal test design for optimization of the extraction of polysaccharide from Paeonia sinjiangensis K.Y. Pan

    Directory of Open Access Journals (Sweden)

    Shuge Tian

    2011-01-01

    Full Text Available Background: Paeonia sinjiangensis K.Y. Pan is a perennial herb belonging to the ranunculaceae family and it is one of the most important crude drugs in traditional Chinese medicine. In this article, Paeonia sinjiangensis K.Y. Pan rich in polysaccharide is used as an experimental material. Materials and Method: Study the effects of proportion, temperature, times and time taken for the extraction yield of polysaccharide through a single-factor exploration. Then, through an orthogonal experiment (L9(34, it was investigated to get the best extraction conditions. Results: The results showed that the ratio of solvent to raw material, number of extractions and duration of extraction were the main variables that influenced the yields of extracts. The separation procedure of precipitation with alcohol and the purification from the removing proteins were deeply analyzed. Meanwhile the contents of polysaccharide were determined by anthrone colorimetry. Conclusion: The highest yield was obtained when the ratio of solvent to raw material, number of extractions, and duration of extraction were 8:1, 2, and 1.5 h, respectively. The content of soluble polysaccharide is 51.57%.

  16. Extractability and digestibility of plant cell wall polysaccharides during hydrothermal and enzymatic degradation of wheat straw (Triticum aestivum L.)

    DEFF Research Database (Denmark)

    Hansen, Mads A.T.; Ahl, Louise I.; Pedersen, Henriette L.

    2014-01-01

    , regardless their extractability in water or only alkali. Based on the results, AX and MLG appear to be loosely bound in the cell wall matrix while the other polysaccharides are bound more tightly and shielded from enzymatic attack by AX and MLG until pretreatment. The gradual solubilisation and digestion...... and by comprehensive microarray polymer profiling (CoMPP). This way, the effects of each degradation step to the intermolecular organisation of specific polysaccharides in the cell walls were elucidated. After pretreatment, the degree of polymerisation (DP) of released xylo-oligosaccharides in both samples was up...... to about 20, but mostly around 3-8, and notably more acetylated in stems. Arabinoxylan (AX) and mixed-linkage glucan (MLG) became water-extractable while xylan, xyloglucan (XG), mannan and glucan remained only alkali-extractable. All polysaccharides became partly digestible after pretreatment however...

  17. Structural Features of Alkaline Extracted Polysaccharide from the Seeds of Plantago asiatica L. and Its Rheological Properties

    Directory of Open Access Journals (Sweden)

    Jun-Yi Yin

    2016-09-01

    Full Text Available Polysaccharide from the seeds of Plantago asiatica L. has many bioactivities, but few papers report on the structural and rheological characteristics of the alkaline extract. The alkaline extracted polysaccharide was prepared from seeds of P. asiatica L. and named herein as alkaline extracted polysaccharide from seeds of P. asiatica L. (PLAP. Its structural and rheological properties were characterized by monosaccharide composition, methylation, GC-MS and rheometry. PLAP, as an acidic arabinoxylan, was mainly composed of 1,2,4-linked Xylp and 1,3,4-linked Xylp residues. PLAP solution showed pseudoplastic behavior, and weak gelling properties at high concentration. Sodium and especially calcium ions played a significant role in increasing the apparent viscosity and gel strength.

  18. Effect of the Polysaccharide Extract from the Edible Mushroom Pleurotus ostreatus against Infectious Bursal Disease Virus

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    Tatiana Rugea

    2009-08-01

    Full Text Available The polysaccharide-containing extracellular fractions (EFs of the edible mushroom Pleurotus ostreatus have immunomodulating effects. Being aware of these therapeutic effects of mushroom extracts, we have investigated the synergistic relations between these extracts and BIAVAC and BIAROMVAC vaccines. These vaccines target the stimulation of the immune system in commercial poultry, which are extremely vulnerable in the first days of their lives. By administrating EF with polysaccharides from P. ostreatus to unvaccinated broilers we have noticed slow stimulation of maternal antibodies against infectious bursal disease (IBD starting from four weeks post hatching. For the broilers vaccinated with BIAVAC and BIAROMVAC vaccines a low to almost complete lack of IBD maternal antibodies has been recorded. By adding 5% and 15% EF in the water intake, as compared to the reaction of the immune system in the previous experiment, the level of IBD antibodies was increased. This has led us to believe that by using this combination of BIAVAC and BIAROMVAC vaccine and EF from P. ostreatus we can obtain good results in stimulating the production of IBD antibodies in the period of the chicken first days of life, which are critical to broilers’ survival. This can be rationalized by the newly proposed reactivity biological activity (ReBiAc principles by examining the parabolic relationship between EF administration and recorded biological activity.

  19. Isolation and structural characterization of the water-extractable polysaccharides from Cassia obtusifolia seeds.

    Science.gov (United States)

    Shang, Mingsheng; Zhang, Xiaoman; Dong, Qun; Yao, Jian; Liu, Qin; Ding, Kan

    2012-10-01

    The seed of Cassia obtusifolia is a food or herbal medicine used for improving eyesight, treating constipation and other disorders, and polysaccharides have been implicated in these pharmacological activities. The endosperm of the seeds, Cassia gum, is a commercial thickening or gelling agent, composed mainly of galactomannans. However, the whole seeds of C. obtusifolia, rather than the endosperm, are used in folk medicine or food, which might contain more complex constituents of polysaccharides. In this study, the whole seeds of C. obtusifolia were extracted with boiling water, and from the water extract, three homogeneous fractions were isolated, designated CFAA-1, CFAA-3, and CFBB2, respectively, after treatment with Fehling solution followed by anion-exchange and gel permeation chromatography. Using chemical and spectroscopic methods, CFAA-1, and CFAA-3 were elucidated to be both branched galactomannans with different molecular weights, consisting of 1,4-linked β-d-mannopyranosyl backbone with single-unit α-d-galactopyranosyl branches attached to O-6 of mannose, while CFBB2 was shown to be a linear (1→4)-α-polygalacturonic acid.

  20. Effect of the Polysaccharide Extract from the Edible Mushroom Pleurotus ostreatus against Infectious Bursal Disease Virus

    Science.gov (United States)

    Selegean, Mircea; Putz, Mihai V.; Rugea, Tatiana

    2009-01-01

    The polysaccharide-containing extracellular fractions (EFs) of the edible mushroom Pleurotus ostreatus have immunomodulating effects. Being aware of these therapeutic effects of mushroom extracts, we have investigated the synergistic relations between these extracts and BIAVAC and BIAROMVAC vaccines. These vaccines target the stimulation of the immune system in commercial poultry, which are extremely vulnerable in the first days of their lives. By administrating EF with polysaccharides from P. ostreatus to unvaccinated broilers we have noticed slow stimulation of maternal antibodies against infectious bursal disease (IBD) starting from four weeks post hatching. For the broilers vaccinated with BIAVAC and BIAROMVAC vaccines a low to almost complete lack of IBD maternal antibodies has been recorded. By adding 5% and 15% EF in the water intake, as compared to the reaction of the immune system in the previous experiment, the level of IBD antibodies was increased. This has led us to believe that by using this combination of BIAVAC and BIAROMVAC vaccine and EF from P. ostreatus we can obtain good results in stimulating the production of IBD antibodies in the period of the chicken first days of life, which are critical to broilers’ survival. This can be rationalized by the newly proposed reactivity biological activity (ReBiAc) principles by examining the parabolic relationship between EF administration and recorded biological activity. PMID:20111675

  1. Safety evaluation of turmeric polysaccharide extract: assessment of mutagenicity and acute oral toxicity.

    Science.gov (United States)

    Velusami, Chandrasekaran Chinampudur; Boddapati, Srinivasa Rao; Hongasandra Srinivasa, Srikanth; Richard, Edwin Jothie; Joseph, Joshua Allan; Balasubramanian, Murali; Agarwal, Amit

    2013-01-01

    Curcuma longa Linn. (Zingiberaceae) commonly known as turmeric has long been used for centuries as a spice and household remedy. The present study was carried out to assess the possible mutagenic potential and acute oral toxicity of polysaccharide extract of turmeric rhizome (NR-INF-02) using standard tests. The standard battery of in vitro genotoxicity tests, bacterial reverse mutation test (BRMT), chromosome aberration (CA), and micronucleus (MN) tests were employed to assess the possible mutagenic activity of NR-INF-02 (Turmacin). The results showed no mutagenic effect with NR-INF-02 up to a dose of 5000 µg/mL in BRMT. The results on CA and MN tests revealed the non clastogenic activity of NR-INF-02 in a dose range of 250.36 to 2500 µg/mL with and without metabolic activation (S9). In acute oral toxicity study, NR-INF-02 was found to be safe up to 5 g/kg body weight in Wistar rats. Overall, results indicated that polysaccharide extract of C. longa was found to be genotoxically safe and also exhibited maximum tolerable dose of more than 5 g/kg rat body weight.

  2. Safety Evaluation of Turmeric Polysaccharide Extract: Assessment of Mutagenicity and Acute Oral Toxicity

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    Chandrasekaran Chinampudur Velusami

    2013-01-01

    Full Text Available Curcuma longa Linn. (Zingiberaceae commonly known as turmeric has long been used for centuries as a spice and household remedy. The present study was carried out to assess the possible mutagenic potential and acute oral toxicity of polysaccharide extract of turmeric rhizome (NR-INF-02 using standard tests. The standard battery of in vitro genotoxicity tests, bacterial reverse mutation test (BRMT, chromosome aberration (CA, and micronucleus (MN tests were employed to assess the possible mutagenic activity of NR-INF-02 (Turmacin. The results showed no mutagenic effect with NR-INF-02 up to a dose of 5000 µg/mL in BRMT. The results on CA and MN tests revealed the non clastogenic activity of NR-INF-02 in a dose range of 250.36 to 2500 µg/mL with and without metabolic activation (S9. In acute oral toxicity study, NR-INF-02 was found to be safe up to 5 g/kg body weight in Wistar rats. Overall, results indicated that polysaccharide extract of C. longa was found to be genotoxically safe and also exhibited maximum tolerable dose of more than 5 g/kg rat body weight.

  3. Optimization Extraction, Preliminary Characterization and Antioxidant Activities of Polysaccharides from Semen Juglandis

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    Xueyong Ren

    2016-10-01

    Full Text Available The optimization extraction process, preliminary characterization and antioxidant activities of polysaccharides from Semen Juglandis (SJP were studied in this paper. Based on the Box-Behnken experimental design and response surface methodology, the optimal extraction conditions for the SJP extraction were obtained as follows: temperature 88 °C, extraction time 125 min and ratio of liquid to solid 31 mL/g. Under these conditions, experimental extraction yield of SJP was (5.73 ± 0.014% (n = 5, similar to the predicted value of 5.78%. Furtherly, the purified SJP obtained from SJP extract by DEAE-52 and Sephacryl S-100 chromatography was analyzed to be rhamnose, galacturonic acid, galactose, arabinose and fucose in the molar ratio of 1:6.34:1.38:3.21:1.56. And the weight-average molecular weight and radius of gyration of the purified SJP in 0.1 M NaCl were determined to be 2.76 × 104 g/mol and 122 nm by SEC-MALLS, respectively. More importantly, it exhibited appreciable antioxidant activities compared to the standard Vc, such as DPPH radical scavenging activity (IC50 0.21 mg/mL, strong reducing power, ABTS radical scavenging activity (IC50 0.29 mg/mL, and hydroxyl radical scavenging activity (IC50 0.38 mg/mL. These results indicate that SJP may be useful for developing functional health products or natural antioxidant.

  4. Optimization of extraction, characterization and antioxidant activity of polysaccharides from Brassica rapa L.

    Science.gov (United States)

    Wang, Wei; Wang, Xiaoqing; Ye, Hong; Hu, Bing; Zhou, Li; Jabbar, Saqib; Zeng, Xiaoxiong; Shen, Wenbiao

    2016-01-01

    The root of Brassica rapa L. has been traditionally used as a Uyghur folk medicine to cure cough and asthma by Uyghur nationality in Xinjiang Uygur Autonomous Region of China. In the present study, therefore, extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from the root of B. rapa L. (BRP) were investigated. The optimal extraction conditions with an extraction yield of 21.48 ± 0.41% for crude BRP were obtained as follows: extraction temperature 93°C, extraction time 4.3h and ratio of extraction solvent (water) to raw material 75 mL/g. The crude BRP was purified by chromatographic columns of DEAE-52 cellulose and Sephadex G-100, affording three purified fractions of BRP-1-1, BRP-2-1 and BRP-2-2 with average molecular weight of 1510, 1110 and 838 kDa, respectively. Monosaccharide composition analysis indicated that BRP-1-1 was composed of mannose, rhamnose, glucose, galactose and arabinose, BRP-2-1 was composed of rhamnose, galacturonic acid, galactose and arabinose, and BRP-2-2 was composed of rhamnose and galacturonic acid in a molar ratio of 1.27: 54.92. Furthermore, the crude BRP exhibited relatively higher antioxidant activity in vitro than purified fractions; hence, it could be used as a natural antioxidant in functional foods or medicines.

  5. One-step column chromatographic extraction with gradient elution followed by automatic separation of volatiles, flavonoids and polysaccharides from Citrus grandis.

    Science.gov (United States)

    Han, Han-Bing; Li, Hui; Hao, Rui-Lin; Chen, Ya-Fei; Ni, He; Li, Hai-Hang

    2014-02-15

    Citrus grandis Tomentosa is widely used in traditional Chinese medicine and health foods. Its functional components include volatiles, flavonoids and polysaccharides which cannot be effectively extracted through traditional methods. A column chromatographic extraction with gradient elution was developed for one-step extraction of all bioactive substances from C. grandis. Dried material was loaded into a column with petroleum ether: ethanol (8:2, PE) and sequentially eluted with 2-fold PE, 3-fold ethanol: water (6:4) and 8-fold water. The elutes was separated into an ether fraction containing volatiles and an ethanol-water fraction containing flavonoids and polysaccharides. The later was separated into flavonoids and polysaccharides by 80% ethanol precipitation of polysaccharides. Through this procedure, volatiles, flavonoids and polysaccharides in C. grandis were simultaneously extracted at 98% extraction rates and simply separated at higher than 95% recovery rates. The method provides a simple and high-efficient extraction and separation of wide range bioactive substances.

  6. Extraction of polysaccharides from herbal Scutellaria barbata D. Don (Ban-Zhi-Lian) and their antioxidant activity.

    Science.gov (United States)

    Ye, Chun-Lin; Huang, Qi

    2012-08-01

    The response surface methodology was employed to study the extraction of polysaccharides from Scutellaria barbata D. Don. The quantitative effects of extraction temperature, time, number and ratio of water to raw material on yield of polysaccharides were investigated with Box-Behnken design. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed using the appropriate statistical methods. By solving the regression equation and analyzing 3D plots, the optimum condition was at extraction temperature 70 °C, time 3h, numbers 3 and ratio of water to raw material 18.5 mL/g. Under these conditions, the experimental polysaccharides yield was 2.43±0.11%, which was in good agreement with the predicted value. The antioxidant activities of the polysaccharides were evaluated in vitro. A potential antioxidant activity of S. barbata polysaccharides provides a scientific basis for the use of this herb in traditional medicine as an antioxidant.

  7. Optimization of enzyme assisted extraction of Fructus Mori polysaccharides and its activities on antioxidant and alcohol dehydrogenase.

    Science.gov (United States)

    Deng, Qingfang; Zhou, Xin; Chen, Huaguo

    2014-10-13

    In the present study, enzyme assisted extraction of Fructus Mori polysaccharides (FMPS) from F. mori using four kinds of enzymes and three compound enzymes were examined. Research found that glucose oxidase offered a better performance in enhancement of the extraction yields of FMPS, antioxidant and activate alcohol dehydrogenase activities. The glucose oxidase assisted extraction process was further optimized by using response surface method (RSM) to obtain maximum yield of crude FMPS. The results showed that optimized extraction conditions were ratio of enzyme amount 0.40%, enzyme treated time 38 min, treated temperature 58 °C and liquid-solid radio 11.0. Under these conditions, the mean experimental value of extraction yield (16.16 ± 0.14%) corresponded well with the predicted values and increased 160% than none enzyme treated ones. Pharmacological verification tests showed that F. mori crude polysaccharides had good antioxidant and activate alcohol dehydrogenase activities in vitro. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Separation and Extraction of Polysaccharide from Schisandra chinensis%五味子多糖的提取及分离

    Institute of Scientific and Technical Information of China (English)

    丛娟; 宫莉; 闫小娟

    2011-01-01

    五味子是一种具有广阔开发前景的传统中药材.实验中,以五味子为原料,利用水提醇沉法得到了五味子粗多糖.在料液质量体积比为1g∶32 mL,浸提温度99℃,提取时间4 h的适宜的提取工艺条件下,粗多糖的提取率达5.61%.采用酶法与Sevag法联用脱除粗多糖中蛋白质效果较好.利用凝胶色谱柱层析法对多糖半纯品进行分级分离,得到了两种多糖Ⅰ和Ⅱ.经薄层色谱分析,初步确定了两种多糖的单糖组成,又通过红外光谱分析,证明多糖Ⅰ可能为α-呋喃糖,多糖Ⅱ可能为β-吡喃糖.%In this manuscript, the optimum conditions of polysaccharide purify from Schisandra, a kind of broad development prospect of traditional Chinese herbal medicines, were determined.Schisandra polysaccharide was extracted by using water extraction and alcohol precipitationand the optimum extraction parameters listed as follows: the ratio of solid-liquid was 1 g: 32 mL,extraction temperature was 99 ℃, extraction time was 4 h.With the optimum conditions, the extraction rate of crude polysaccharide could reach at 5.61%.furthermore, the protein of crude polysaccharide was removed by using enzymatic and savage method to eliminate.Semi-pure polysaccharide was graded and separated by gel column chromatography, two kinds of polysaccharide Ⅰ and Ⅱ obtained.After thin-layer chromatography analysis, the composition of polysaccharides was preliminary identified, through infrared spectroscopy analysis,polysaccharide Ⅰ and Ⅱ were identified as α-Furan sugar and β-pyranose, respectively.

  9. Anti-Inflammatory Activity of Polysaccharide Fraction of Curcuma longa Extract (NR-INF-02).

    Science.gov (United States)

    Illuri, Ramanaiah; Bethapudi, Bharathi; Anandakumar, Senthilkumar; Murugan, Sasikumar; Joseph, Joshua A; Mundkinajeddu, Deepak; Agarwal, Amit; Chandrasekaran, C V

    2015-01-01

    The aim of the study was to investigate the safety and anti-inflammatory effects of polysaccharide fraction (F1) of Curcuma longa extract (NR-INF-02) in classical rodent models of inflammation. F1 was evaluated for its acute oral toxicity and found to be safe upto 5000 mg/kg body weight in rats. The anti-inflammatory activity of F1 was evaluated in acute (carrageenan - induced paw edema; xylene - induced ear edema) and chronic (cotton pellet - induced granuloma) models of inflammation. The results of the study demonstrated that F1 significantly (p ≤ 0.05) inhibited carrageenan-induced paw edema at 1 h and 3 h at doses of 11.25, 22.5 and 45 mg/kg body weight in rats. Also, F1 at doses of 15.75, 31.5 and 63 mg/kg significantly inhibited the xylene induced ear edema in mice. In a chronic model, F1 at 11.25, 22.5 and 45 mg/kg doses produced significant reduction of wet and dry weights of cotton pellets in rats. Overall results indicated that F1 of NR-INF-02 significantly attenuated acute and chronic inflammation in rodent models. This study emphasizes on the importance of Curcuma longa polysaccharide's role in acute and chronic inflammation.

  10. Optimisation of pressurised water extraction of polysaccharides from blackcurrant and its antioxidant activity.

    Science.gov (United States)

    Xu, Yaqin; Cai, Fei; Yu, Zeyuan; Zhang, Ling; Li, Xingguo; Yang, Yu; Liu, Gaijie

    2016-03-01

    Pressurised water extraction (PWE) of polysaccharides from blackcurrant fruits was investigated using a response surface methodology (RSM). The optimal conditions for PWE were: time 51min, pressure 1.6MPa, and temperature 52°C. Under these conditions, the experimental yield of Ribes nigrum L. polysaccharides (RNLP) was 11.68±0.12%, which closely agreed with the predicted value (11.77%). After preliminary purification with D4006 macroporous resin, RNLP I was obtained and its chemical characterisation was undertaken by GC, HPLC, and IR spectroscopy. RNLP I was composed of rhamnose, arabinose, xylose, mannose, galactose, and glucose with a molar ratio of 2.89:14.82:1.02:1.00:2.53:6.39 and its molecular weight was 1.49×10(4)kDa. The antioxidant activity of RNLP I was evaluated by free radical scavenging assays and a reducing power assay in vitro. RNLP I showed strong DPPH and superoxide radical scavenging activities and reducing power.

  11. Optimization of ultrasound-assisted compound enzymatic extraction and characterization of polysaccharides from blackcurrant.

    Science.gov (United States)

    Xu, Yaqin; Zhang, Ling; Yang, Yu; Song, Xiumei; Yu, Zeyuan

    2015-03-06

    In the present study, an efficient procedure for ultrasound-assisted compound enzymatic extraction of polysaccharides from blackcurrant fruits was investigated using response surface methodology (RSM). The Box-Behnken design was applied to optimize the effects of enzyme concentration (X1), pH (X2) and ultrasonic time (X3). The statistical analysis indicated that the independent variables (X1) and the quadratic terms (X1(2) and X3(2)) had significant effects on the yield of blackcurrant polysaccharides (BCP). The optimal conditions were: enzyme concentration 1.575%, pH 5.3, and ultrasonic time 25.6 min. The experimental yield of BCP was 14.28±0.06%, which was closely matched with the predicted yield of 14.31%. After preliminary purification, BCP I was obtained and characterized by GC, HPLC, and IR. BCP I comprised rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 1.818:1.362:0.377:0.501:1.581:1.722 and its molecular weight was 8146 kDa. BCP I showed notable α-amylase inhibitory activity. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Antioxidant and anti-aging effects of acidic-extractable polysaccharides by Agaricus bisporus.

    Science.gov (United States)

    Li, Shangshang; Liu, Hui; Wang, Wenshuai; Wang, Xiuxiu; Zhang, Chen; Zhang, Jianjun; Jing, Huijuan; Ren, Zhenzhen; Gao, Zheng; Song, Xinling; Jia, Le

    2017-09-01

    This study was designed to investigate the antioxidant and anti-aging effects of D-galatose-induced (D-gal-induced) aging mice as well as monosaccharide compositions of acidic-extractable polysaccharides (AcAPS) and its major purified fractions (AcAPS-1, AcAPS-2 and AcAPS-3) from the fruiting body of Agaricus bisporus. In the in vitro assays, AcAPS-2 showed superior scavenging activities on hydroxyl (82.98±4.67%) and DPPH (64.47±4.05%) radicals at the concentration of 1.0mg/mL than AcAPS and other polysaccharides fractions. For in vivo anti-aging analysis, AcAPS-2 showed superior effects on hepatic and nephric protection by improving serum enzyme activities, biochemical levels, lipid contents and antioxidant status, respectively. The monosaccharide analysis showed that rhamnose (Rha) and glucose (Glu) may play vital roles in maintaining the antioxidant and anti-aging activities. The results suggested that both AcAPS and its purified fractions might be suitable for functional foods and natural drugs in preventing the acute aging-associated diseases. Copyright © 2017 Elsevier B.V. All rights reserved.

  13. Anti-tumor and macrophage activation induced by alkali-extracted polysaccharide from Pleurotus ostreatus.

    Science.gov (United States)

    Kong, Fanli; Li, Feng-E; He, Zhongmei; Jiang, Yong; Hao, Ruoyi; Sun, Xin; Tong, Haibin

    2014-08-01

    Pleurotus ostreatus is popularly consumed as traditional medicine and health food for enhancing immune function in China. Polysaccharides from mushroom have been demonstrated to possess a wide range of health beneficial properties. This study was carried out to elucidate the immunomodulating effects and molecular mechanism involved in the in vivo and in vitro anti-tumor activities of alkali-extracted polysaccharide (WPOP-N1) from the fruiting bodies of P. ostreatus. The results showed that WPOP-N1 significantly inhibited the tumor growth of Sarcoma 180 tumor-bearing mice, and markedly increased the secretion level of TNF-α in serum. In addition, WPOP-N1 enhanced the phagocytic capability of peritoneal macrophages in vitro. Furthermore, the secretion of TNF-α and NO and the amount of TNF-α and iNOS transcript were increased significantly when the peritoneal macrophages were exposed to WPOP-N1. Meanwhile, Western blot analysis revealed that the stimulation of peritoneal macrophages by WPOP-N1 induced the phosphorylation of p65 and a marked decrease of IκB expression. These results suggest that WPOP-N1 could activate macrophages through NF-κB signaling pathway, and the anti-tumor effects of WPOP-N1 can be achieved by its immunostimulating property. Copyright © 2014 Elsevier B.V. All rights reserved.

  14. Extraction, characterization, and biological activity of polysaccharides from Sophora flavescens Ait.

    Science.gov (United States)

    Zhang, Qihong; Yu, Jingbo; Zhang, Leifang; Hu, Meiqun; Xu, Yan; Su, Weike

    2016-12-01

    Four water-soluble polysaccharides, designated as SF1, SF2, SF3 and SF4, were efficiently extracted from the roots of Sophora flavescens by mechanochemistry under the conditions of rotational speed of 400rpm, grinding time of 10min, powder to ball weight ratio of 1:20, and Na2CO3 loading of 7wt%. The results obtained indicated that all of these four acid heteropolysaccharides are composed of rhamnose, arabinose, xylose, mannose, glucose and galactose, with the average molecular weights of 400.9, 98.6, 99.3, 42.7kDa, respectively. In vitro, SF4 showed the most significant scavenging activity on superoxide radical, ABTS, and DPPH radical, while SF3 had the most significant scavenging activity on hydroxyl radical. Immunological tests demonstrated that SF1, SF2, SF3 and SF4 significantly stimulated nitric oxide production without cytotoxicity in macrophages and promoted splenocyte proliferation. These data suggest that the four polysaccharides fractions have the potential as novel natural sources of antioxidative and immunopotentiating agents.

  15. Optimization of Ultrasound-Assisted Extraction, Preliminary Characterization and In Vitro Antioxidant Activity of Polysaccharides from Green Pea Pods.

    Science.gov (United States)

    Jalili Safaryan, Maryam; Ganjloo, Ali; Bimakr, Mandana; Zarringhalami, Soheila

    2016-11-28

    In this study, ultrasound-assisted extraction of green pea pod polysaccharide (GPPP) was investigated and optimized using a central composite response surface design coupled with a numerical optimization technique. The effects of ultrasonic power (50-150 W), sonication time (20-80 min), ratio of water to raw material (20:1-40:1 mL/g) and extraction temperature (40-80 °C) on polysaccharide extraction yield were studied. The maximum extraction yield was obtained with a sonication power of 135.34 W, extraction time of 48.61 min, ratio of water to raw material of 33.6:1 mL/g and extraction temperature of 68.25 °C. Under these conditions, the experimental yield was 7.37% ± 0.13%, which was in close agreement with the predicted value (7.20%). The GPPP has been analyzed in order to identify a variety of chemical properties. The FT-IR spectrum demonstrated obvious characteristic peaks of polysaccharides. Furthermore, antioxidant activity of GPPP was evaluated by various antioxidant assays in vitro. The results revealed that GPPP possessed considerable DPPH free radical scavenging activity (91.03%), reducing power (0.63) and ferric reducing antioxidant power (0.34 mmol/L) at a total amount of 0.9 mg/mL. These findings indicated that GPPP extracted using an ultrasound-assisted extraction technique has potential as a novel source of natural antioxidant agent for future applications.

  16. Microwave-Assisted Extraction, Chemical Structures, and Chain Conformation of Polysaccharides from a Novel Cordyceps Sinensis Fungus UM01.

    Science.gov (United States)

    Cheong, Kit-Leong; Wang, Lan-Ying; Wu, Ding-Tao; Hu, De-Jun; Zhao, Jing; Li, Shao-Ping

    2016-09-01

    Cordyceps sinensis is a well-known tonic food with broad medicinal properties. The aim of the present study was to investigate the optimization of microwave-assisted extraction (MAE) and characterize chemical structures and chain conformation of polysaccharides from a novel C. sinensis fungus UM01. Ion-exchange and gel filtration chromatography were used to purify the polysaccharides. The chemical structure of purified polysaccharide was determined through gas chromatography-mass spectrometry. Moreover, high performance size exclusion chromatography combined with refractive index detector and multiangle laser light scattering were conducted to analyze the molecular weight (Mw ) and chain conformation of purified polysaccharide. Based on the orthogonal design L9 , optimal MAE conditions could be obtained through 1300 W of microwave power, with a 5-min irradiation time at a solid to water ratio of 1:60, generating the highest extraction yield of 6.20%. Subsequently, the polysaccharide UM01-S1 was purified. The UM01-S1 is a glucan-type polysaccharide with a (1→4)-β-d-glucosyl backbone and branching points located at O-3 of Glcp with a terminal-d-Glcp. The Mw , radius of gyration (Rg ) and hydrodynamic radius (Rh ) of UM01-S1 were determined as 5.442 × 10(6)  Da, 21.8 and 20.2 nm, respectively. Using the polymer solution theory, the exponent (ν) value of the power law function was calculated as 0.38, and the shape factor (ρ = Rg /Rh ) was 1.079, indicating that UM01-S1 has a sphere-like conformation with a branched structure in an aqueous solution. These results provide fundamental information for the future application of polysaccharides from cultured C. sinensis in health and functional food area. © 2016 Institute of Food Technologists®

  17. Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.

    Science.gov (United States)

    Wu, Zhongwei; Zhang, Mingxia; Xie, Minhao; Dai, Zhuqing; Wang, Xiaoqing; Hu, Bing; Ye, Hong; Zeng, Xiaoxiong

    2016-02-10

    Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated. As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g. The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively. Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group. These results suggested that PHMPs could be explored as promising natural antioxidant.

  18. Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus

    NARCIS (Netherlands)

    Kozarski, M.; Klaus, A.; Niksic, M.; Jakovljevic, D.; Helsper, J.P.F.G.; Griensven, van L.J.L.D.

    2011-01-01

    Partially purified polysaccharides were obtained from four medicinal mushroom species, Agaricus bisporus, Agaricus brasiliensis, Phellinus linteus and Ganoderma lucidum by hot water extraction, followed by ethanol precipitation. The four samples contained varying amounts of both a- and ß-glucans as

  19. Phellinus linteus polysaccharide extracts increase the mitochondrial membrane potential and cause apoptotic death of THP-1 monocytes

    NARCIS (Netherlands)

    Griensven, van L.J.L.D.; Verhoeven, H.A.

    2013-01-01

    Background: The differentiation resp. death of human monocytic THP-1 cells induced by polysaccharide extracts of the medicinal mushrooms Phellinus linteus, Agaricus bisporus and Agaricus brasiliensis have been studied. This study aims to identify leads for the causal effects of these mushroom compon

  20. Immunomodulatory Potential of the Polysaccharide-Rich Extract from Edible Cyanobacterium Nostoc commune

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    Hui-Fen Liao

    2015-11-01

    Full Text Available A dry sample of Nostoc commune from an organic farm in Pingtung city (Taiwan was used to prepare polysaccharide-rich (NCPS extract. The conditioned medium (CM from NCPS-treated human peripheral blood (PB-mononuclear cells (MNC effectively inhibited the growth of human leukemic U937 cells and triggered differentiation of U937 monoblast cells into monocytic/macrophagic lines. Cytokine levels in MNC-CMs showed upregulation of granulocyte/macrophage-colony stimulatory factor and IL-1β and downregulation of IL-6 and IL-17 upon treatment with NCPS. Moreover, murine macrophage RAW264.7 cells treated with NCPS exhibited the stimulatory effects of nitric oxide and superoxide secretion, indicating that NCPS might activate the immunity of macrophages. Collectively, the present study demonstrates that NCPS from N. commune could be potentially used for macrophage activation and consequently inhibited the leukemic cell growth and induced monocytic/macrophagic differentiation.

  1. Optimization of extraction process and investigation of antioxidant effect of polysaccharides from the root of Limonium sinense Kuntze

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    Xinhui Tang

    2011-01-01

    Full Text Available Objective: To optimize the extraction technology for polysaccharides from the root of Limonium sinense (Girard Kuntze, Plumbaginaceae and evaluate the antioxidant capacity of polysaccharides from L. sinense (LSEP Materials and Methods : One-singer factor and response surface methodology(RSM were established to extract the polysaccharides from L. sinense. Then, the 1,1-diphenyl-2-picrylhydrazyl free radical, hydroxyl radical(.OH, and 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt free radical assays were established to measure the antioxidant capacity of the LSEP in vitro. Results: According to analysis, extraction temperature significantly affected extraction yield. The optimum extraction conditions for LSEP were as follows: extraction temperature, 95°C; ultrasonic time 50 minutes; and dosage liquor ratio, 1: 12. Under these conditions, the experimental yield of crude LSEP was 12.80±0.19% which was well matched with the predicted models. The antioxidant capacity data suggested that LSEP has strong antioxidant activity. Conclusion: One-singer factor and RSM were used to extract of LSEP are simple and feasible and LSEP could be developed as a nutraceutical agent for itsstrong antioxidant activity.

  2. In vitro cancer chemopreventive properties of polysaccharide extract from the brown alga, Sargassum latifolium.

    Science.gov (United States)

    Gamal-Eldeen, Amira M; Ahmed, Eman F; Abo-Zeid, Mona A

    2009-06-01

    Polysaccharides of edible algae attracted extensive interest due to their numerous biological activities. Sargassum latifolium (Turner) C. Agardh, belongs to Sargassaceae, is a brown algae in red sea shores in Egypt. This work is a novel attempt to explore the cancer chemopreventive activity of different fractions of water-soluble polysaccharide extract derived from S. latifolium. Estimation of cancer chemopreventive activity, specifically anti-initiation, including the modulation of carcinogen metabolism and the antioxidant capacity, revealed that E1 and E4 were potent anti-initiators, where they lead not only to an inhibition in the carcinogen activator cytochrome P450 1A (IC50 2.54 and 10.30 microg/ml, respectively), but also to an induction in the carcinogen detoxification enzymes glutathione-S-transferases (144% and 225% of the control, respectively). E1 and E4 inhibited 59% and 63% of the induced-DNA damage, as measured by comet assay. Similarly both E1 and E4 possessed potential anti-promoting properties as indicated by their anti-inflammatory activity. E1 and E4 enhanced the macrophage proliferation; however they dramatically inhibited the stimulated NO (30.7% and 59.3%), TNF-alpha (38.2% and 54.9) and COX-2 (20% and 18%), respectively. E3 showed a selective cytotoxicity against lymphoblastic leukemia (1301 cells), while other fraction extracts had no cytotoxic effect against all tested cell lines. E3 led to a major disturbance in cell cycle including arrest in both S-phases in 1301 cells. This disturbance was associated with an induced-cell death due to apoptosis, but not necrosis. In conclusion, E1 and E4 are promising cancer chemopreventive fractions, since they had tumor anti- initiating activity via their protective modulation of carcinogen metabolism, and tumor anti-promoting activity via their anti-inflammatory activity, while E3 can be considered as a promising anti-cancer agent against leukemia.

  3. Immune-stimulatory and anti-inflammatory activities of Curcuma longa extract and its polysaccharide fraction

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    Chinampudur V Chandrasekaran

    2013-01-01

    Full Text Available Background: While curcuminoids have been reported to possess diverse biological activities, the anti-inflammatory activity of polar extracts (devoid of curcuminoids of Curcuma longa (C. longa has seldom been studied. In this study, we have investigated immune-stimulatory and anti-inflammatory activities of an aqueous based extract of C. longa (NR-INF-02 and its fractions in presence and absence of mitogens. Materials and Methods: Effects of NR-INF-02 (Turmacin TM , Natural Remedies Pvt. Ltd., Bangalore, India on proliferation, nitric oxide (NO, monocyte chemotactic protein-1 (MCP-1, interleukins (ILs and prostaglandin (PGE 2 levels of mouse splenocytes and mouse macrophage (RAW264.7 cells were determined. Results: NR-INF-02 increased splenocytes number in presence and absence of lipopolysaccharide (LPS or concanavalin A. Treatment of NR-INF-02 showed a significant increase of NO, IL-2, IL-6, IL-10, IL-12, interferon (IFN gamma, tumor necrosis factor (TNF alpha and MCP-1 production in unstimulated mouse splenocytes and mouse macrophages. Interestingly, NR-INF-02 showed potent inhibitory effect towards release of PGE 2 and IL-12 levels in LPS stimulated mouse splenocytes. Further, NR-INF-02 was fractionated into polysaccharide fraction (F1 and mother liquor (F2 to study their immune-modulatory effects. F1 was found to be more potent than F2 toward inhibiting PGE 2 and IL-12 in LPS stimulated splenocytes. Conclusion: Present findings revealed the novel anti-inflammatory property of NR-INF-02 and its polysaccharide fraction by inhibiting the secretion of IL-12 and PGE 2 in vitro.

  4. Optimization for ultrasound-assisted extraction of polysaccharides with antioxidant activity in vitro from the aerial root of Ficus microcarpa.

    Science.gov (United States)

    Jiang, Changxing; Li, Xia; Jiao, Yunpeng; Jiang, Dingyun; Zhang, Ling; Fan, Benxia; Zhang, Qianghua

    2014-09-22

    In this study, optimization of ultrasound-assisted extraction and antioxidant activity of polysaccharides from the aerial root of Ficus microcarpa (FMPS) were investigated. The optimal conditions for extraction of FMPS were determined as followings: ultrasound power 200 W, ultrasound temperature 70°C, extraction temperature 74°C, liquid-solid ratio 35, extraction time 238 min, ultrasound time 49 min. The experimental yield of FMPS (3.44%) obtained under these conditions was well agreement with the value predicted by the model. In addition, Fourier transform-infrared spectroscopy and antioxidant activity assays revealed that FMPS were acidic polysaccharides and had strong Fe2+ chelating activity and moderate hydrogen peroxide scavenging effect. Further work on the purification, structure characterization and antioxidant activity in vivo of FMPS is in progress.

  5. Insights in the antioxidant synergistic effects of combined edible mushrooms: phenolic and polysaccharidic extracts of Boletus edulis and Marasmius oreades

    OpenAIRE

    Vieira, Vanessa; Marques, Azucena; Barros, Lillian; Barreira, João C.M.; Isabel C. F. R. Ferreira

    2012-01-01

    In a previous work, we reported the presence of Marasmius oreades in mixtures with antioxidant synergistic effects, and the mixture Boletus edulis and Marasmius oreades (50% of each) as having the highest antioxidant activity, but without synergism among the phenolic extracts. Herein, phenolic and polysaccharidic extracts from both species were combined in different proportions (12.5, 25, 50, 75 and 87.5%) and compared to controls (individual samples), in order to give insight in ...

  6. Rapid determination of polysaccharides in BianTi Soft extract by spectrophotometry coupled with gas chromatography-mass spectrometry

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    Minxia Zheng

    2010-01-01

    Full Text Available A simple approach for the rapid determination of polysaccharides in BianTi Soft Extract using spectrophotometry coupled with gas chromatography-mass spectrometry (GC-MS was developed. The mixed standard solution composed of D-glucose, D-mannose, galactose and D-xylose in different proportions (1.00: 1.01: 0.12: 0.05 was prepared according to the monosaccharide composition analysis of the polysaccharides by GC-MS. The determination of polysaccharides by UV-Vis spectrophotometer was performed after 35-min color reaction, in which 1 ml 5% phenol and 4 ml sulfate was used. The assay of the method validation has shown that the method was stable, reliable and feasible. Furthermore, the proposed method was successfully applied in the preparation procedure of BianTi Soft Extract, selecting out optimal decoction conditions and suitable decoction container. It suggests that the convenient method could be useful for the quality control of BianTi Soft Extract. Meanwhile, it may be an alternative for polysaccharides determination of other formulations.

  7. Ultrasonic extraction optimization of L. macranthoides polysaccharides and its physicochemical properties.

    Science.gov (United States)

    Wu, Zhen; Li, Hong; Yang, Yong; Tan, Hongjun

    2015-03-01

    The dried flower buds of L. macranthoides, belong to the item Shan Yin Hua, are widely used as raw materials for pharmaceutical, food additive, healthy food and cosmetic industry in China. To optimize the effects of the ultrasonic-assisted extraction (UAE) processing parameters on the yield of L. macranthoides polysaccharides (LMPs), a response surface methodology with a central composite rotatable design was employed. Four independent variables were investigated: ultrasonic power (X1), temperature (X2), time (X3), and the ratio of water volume to raw material weight (W/M ratio, X4). The experimental data were fitted to a quadratic polynomial equation using multiple regression analysis and also examined using appropriate statistical methods. The optimum conditions were: X1, 113.6 W; X2, 71.5°C; X3, 54.7 min; and X4, 30.7 mL/g. Under the optimal conditions, the extraction yield of LMPs was (4.81±0.12)%, which is in close agreement with the value predicted by the statistical model. Further, LMPs were characterized by FT-IR, XRD, TGA/DSC and NMR. In vitro experiments indicated that LMPs had strong scavenging capacities towards the DPPH, hydroxyl and superoxide radicals. Overall, LMPs may have potential applications in the medical and food industries.

  8. Polysaccharide extract of Mimosa tenuiflora stem barks stimulates acute inflammatory response via nitric oxide

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    Kaira Emanuella Sales da Silva-Leite

    2016-12-01

    Full Text Available Mimosa tenuiflora (Mimosaceae or “jurema-preta” is well distributed in the northeast Brazil, being popularly used to treat skin lesions, burns and inflammation. The healing effect of the alcoholic extract prepared with its barks corroborates the popular use. This study aimed to evaluate the inflammatory response of polysaccharides extracted from M. tenuiflora barks (EP-Mt by methanol/NaOH and ethanol precipitation. Inflammatory activity was assessed in rat models of acute inflammation (paw edema and peritonitis, by the following parameters: edema, vascular permeability, leukocyte migration, myeloperoxidase activity and pharmacological modulation of nitric oxide and prostaglandins. EP-Mt presented 3.8% yield, 41% carbohydrate and 0.34% protein. EP-Mt (0.01, 0.1, 1.0 mg kg-1 injected by subcutaneous route elicited paw edema that lasted from 30-420 min, with maximal effect at 1 mg kg-1 (40x vs. saline, and was inhibited by L-NAME (52% and dexamethasone (26%. EP-Mt (1 mg kg-1, via intraperitoneal stimulated leukocytes migration (2.2x, mainly neutrophils (6.5x and MPO activity (96%. The leukocyte migration elicited by EP-Mt was inhibited by dexamethasone (39% and L-NAME (38%. EP-Mt containing high carbohydrate content induces acute inflammation via nitric oxide, which open perspectives of application in pathological conditions of immunosuppression.

  9. Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.

    Science.gov (United States)

    Zhang, Jianjun; Meng, Guangyuan; Zhai, Guoyin; Yang, Yongheng; Zhao, Huajie; Jia, Le

    2016-01-01

    To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G. The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88. Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC). Both GPS and its fractions showed potential antioxidant activities by scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and increasing the reducing power in vitro; and by improving the CAT activities, and lowing the LPO and MDA contents in vivo, respectively. The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.

  10. Ultrahigh pressure-assisted enzymatic extraction maximizes the yield of longan pulp polysaccharides and their acetylcholinesterase inhibitory activity in vitro.

    Science.gov (United States)

    Bai, Yajuan; Liu, Lei; Zhang, Ruifen; Huang, Fei; Deng, Yuanyuan; Zhang, Mingwei

    2017-03-01

    An extraction method employing ultrahigh pressure-assisted enzymatic treatment was developed and optimized by response surface methodology to increase the yield of longan pulp polysaccharides (LP-UE). A maximum polysaccharides yield of 8.55% was obtained under the optimal conditions of 407MPa ultrahigh pressure maintained for 6min with an enzyme to pretreated material ratio of 1:100, an enzymolysis time of 1.7h and a water to pretreated material ratio of 42ml/g. Subsequently, the physicochemical properties and acetylcholinesterase (AChE) inhibitory activity of LP-UE were compared to those of longan pulp polysaccharides (LP) extracted by hot water (LP-H), ultrahigh pressure (LP-U) or enzymatic treatment (LP-E). Results demonstrated that the extraction yield, hexuronic acid content and AChE inhibitory activity of LP-UE was the highest among the four LP samples. LP-UE was primarily made up of arabinose, glucose, and galactose and was linked mainly by β-type glycosidic linkage. The FTIR spectrum of LP-UE was very similar to those of LP-H, LP-U, and LP-E. In summary, ultrahigh pressure-assisted enzymatic treatment is a more efficient technique for extracting LP with considerable improvement of both yield and memory enhancement function. Copyright © 2016. Published by Elsevier B.V.

  11. Optimization of Ultrasound-Assisted Extraction, Preliminary Characterization and In Vitro Antioxidant Activity of Polysaccharides from Green Pea Pods

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    Maryam Jalili Safaryan

    2016-11-01

    Full Text Available In this study, ultrasound-assisted extraction of green pea pod polysaccharide (GPPP was investigated and optimized using a central composite response surface design coupled with a numerical optimization technique. The effects of ultrasonic power (50–150 W, sonication time (20–80 min, ratio of water to raw material (20:1–40:1 mL/g and extraction temperature (40–80 °C on polysaccharide extraction yield were studied. The maximum extraction yield was obtained with a sonication power of 135.34 W, extraction time of 48.61 min, ratio of water to raw material of 33.6:1 mL/g and extraction temperature of 68.25 °C. Under these conditions, the experimental yield was 7.37% ± 0.13%, which was in close agreement with the predicted value (7.20%. The GPPP has been analyzed in order to identify a variety of chemical properties. The FT-IR spectrum demonstrated obvious characteristic peaks of polysaccharides. Furthermore, antioxidant activity of GPPP was evaluated by various antioxidant assays in vitro. The results revealed that GPPP possessed considerable DPPH free radical scavenging activity (91.03%, reducing power (0.63 and ferric reducing antioxidant power (0.34 mmol/L at a total amount of 0.9 mg/mL. These findings indicated that GPPP extracted using an ultrasound-assisted extraction technique has potential as a novel source of natural antioxidant agent for future applications.

  12. In vitro antioxidant activities of sulfated derivatives of polysaccharides extracted from Auricularia auricular.

    Science.gov (United States)

    Zhang, Hua; Wang, Zhen-Yu; Yang, Lin; Yang, Xin; Wang, Xue; Zhang, Zhi

    2011-01-01

    In this research, two types of sulfated polysaccharide derivatives were successfully synthesized. Their antioxidant activities were investigated by employing various established in vitro systems. In addition, the degree of sulfation was evaluated using ion-chromatography and IR spectra. The results verify that, when employing scavenging superoxide radical tests, both the sulfation of acid Auricularia auricular polysaccharides (SAAAP) and the sulfation of neutral Auricularia auricular polysaccharides (SNAAP) derivatives possessed considerable antioxidant activity and had a more powerful antioxidant competence than that of the native non-sulfated polysaccharides (AAAP and NAAP). On the other hand, AAAP and NAAP exhibited stronger activity on scavenging both the hydroxyl radical and lipid peroxidation. Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity. In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

  13. In Vitro Antioxidant Activities of Sulfated Derivatives of Polysaccharides Extracted from Auricularia auricular

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    Zhi Zhang

    2011-05-01

    Full Text Available In this research, two types of sulfated polysaccharide derivatives were successfully synthesized. Their antioxidant activities were investigated by employing various established in vitro systems. In addition, the degree of sulfation was evaluated using ion-chromatography and IR spectra. The results verify that, when employing scavenging superoxide radical tests, both the sulfation of acid Auricularia auricular polysaccharides (SAAAP and the sulfation of neutral Auricularia auricular polysaccharides (SNAAP derivatives possessed considerable antioxidant activity and had a more powerful antioxidant competence than that of the native non-sulfated polysaccharides (AAAP and NAAP. On the other hand, AAAP and NAAP exhibited stronger activity on scavenging both the hydroxyl radical and lipid peroxidation. Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity. In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

  14. Optimization of enzymolysis-ultrasonic assisted extraction of polysaccharides from Momordica charabtia L. by response surface methodology.

    Science.gov (United States)

    Fan, Tao; Hu, Jianguo; Fu, Lidan; Zhang, Lijin

    2015-01-22

    An efficient enzymolysis-ultrasonic assisted extraction (EUAE) was developed and optimized for the extraction of polysaccharide from Momordica charabtia L. The single factor experiments and orthogonal experiments were used for the key experimental factors and their test range. Based on the preliminary experimental results, the response surface methodology (RSM) and Box-Behnken design (BBD) were applied for the optimization of EUAE conditions. Using the multiple regression analysis and analysis of variance (ANOVA), the experimental data were fitted to a second-order polynomial equation and were used to generate the mathematical model of optimization experiments. The optimal extraction conditions were as follows: a pH of 4.38, a extraction temperature of 52.02°C and a extraction time of 36.87 min. Under the optimal extraction conditions, the extraction yield of Momordica charabtia L. polysaccharides (MCP) was 29.75±0.48%, which was well matched with the predicted value (29.80%) of the BBD model. Copyright © 2014 Elsevier Ltd. All rights reserved.

  15. Structure and antioxidative property of a polysaccharide from an ammonium oxalate extract of Phellinus linteus.

    Science.gov (United States)

    Yan, Jing-Kun; Wang, Yao-Yao; Wang, Zhen-Bin; Ma, Hai-Le; Pei, Juan-Juan; Wu, Jian-Yong

    2016-10-01

    In this paper, the novel polysaccharide PL-A11 was purified from an ammonium oxalate extract of Phellinus linteus mycelia. Its physicochemical properties, structural characteristics, and antioxidant activities were investigated. Results showed that PL-A11 had a weight-average molecular weight (Mw) of 13.8kDa and was mainly composed of arabinose, xylose, mannose, and glucose in a molar ratio of 1.1:1.3:1.0:6.6. The backbone of PL-A11 was composed of (1→4)-α-d-glucopyranosyl, (1→2)-α-d-xylopyranosyl, and (1→3)-α-d-arabinofuranosyl residues, whereas the (1→6)-α-d-mannopyranosyl residues formed branches at the O-2 position with 1-linked-α-d-glucopyranosyl terminal residues. From the antioxidative activity tests in vivo, the administration of PL-A11 obviously enhanced the activity of antioxidant enzymes and significantly reduced the level of malondiadehyde (MDA) in the serum and liver of d-galactose-treated aging mice in a dose-dependent manner, as well as effectively stimulated the immune system of aging mice. These findings implied that PL-A11 could be developed as a potential antioxidant for applications in the functional food, pharmaceutical, cosmetic or nutraceutical industries.

  16. Structural features and antitumor activity of a novel polysaccharide from alkaline extract of Phellinus linteus mycelia.

    Science.gov (United States)

    Pei, Juan-Juan; Wang, Zhen-Bin; Ma, Hai-Le; Yan, Jing-Kun

    2015-01-22

    A novel high molecular weight polysaccharide (PL-N1) was isolated from alkaline extract of the cultured Phellinus linteus mycelia. The weight average molecular weight (Mw) of PL-N1 was estimated at 343,000kDa. PL-N1 comprised arabinose, xylose, glucose, and galactose in the molar ratio of 4.0:6.7:1.3:1.0. The chemical structure of PL-N1 was investigated by FTIR and NMR spectroscopies and methylation analysis. The results showed that the backbone of PL-N1 comprised (1→4)-linked β-D-xylopyranosyl residues, (1→2)-linked α-D-xylopyranosyl residues, (1→4)-linked α-D-glucopyranosyl residues, (1→5)-linked β-D-arabinofuranosyl residues, (1→4)-linked β-D-xylopyranosyl residues which branched at O-2, and (1→4)-linked β-D-galactopyranosyl residues which branched at O-6. The branches consisted of (1→)-linked α-D-arabinofuranosyl residues. Antitumor activity assay in vitro showed that PL-N1 could inhibit the growth of HepG2 cells to a certain extent in a dose-dependent manner. Thus, PL-N1 may be developed as a potential, natural antitumor agent and functional food.

  17. Immunomodulatory activity of protein-bound polysaccharide extracted from Chelidonium majus.

    Science.gov (United States)

    Song, Jie-Young; Yang, Hyun-Ok; Pyo, Suhk-Neung; Jung, In-Sung; Yi, Seh-Yoon; Yun, Yeon-Sook

    2002-04-01

    In the course of searching immunomodulators from natural sources, the protein-bound polysaccharide, CM-Ala, has been isolated from the water extract of Chelidonium majus L. (Papaveraceae). The immunostimulatory characteristics have been investigated in several experiments such as generation of activated killer (AK) cells, proliferation of splenocytes, activation of macrophages and granulocyte macrophage-colony forming cell (GM-CFC) assay. Of the fractions obtained using Sephacryl S200 column chromatography, CM-Ala was the most effective fraction that augmented the cytotoxicity against Yac-1 tumor cells from 0.88% to 34.18% by culturing with splenocytes for 5 days. CM-Ala also enhanced nitric oxide production by two fold in peritoneal macrophages and exhibited antitumor activity. It showed mitogenic activity on both spleen cells and bone marrow cells. CM-Ala induced proliferation of splenocytes by 84 fold and increased GM-CFC numbers by 1.48 fold over than the non-treated. On the contrary, CM-Ala had cytotoxic activity to a diverse group of tumor cells. From the above results, we proposed that CM-Ala has a possibility of an effective antitumor immunostimulator.

  18. Antioxidant and lipoxygenase activities of polyphenol extracts from oat brans treated with polysaccharide degrading enzymes

    Directory of Open Access Journals (Sweden)

    Nisita Ratnasari

    2017-07-01

    Full Text Available This study used polysaccharide degrading enzymes and protein precipitation to extract polyphenols from oats and to determine their bioactivity. Duplicate oat brans were treated with viscozyme (Vis, cellulase (Cel or no enzyme (control, CTL then, proteins were removed in one set (Vis1, Cel1, CTL1 and not in the other (Vis2, Cel2, CTL2. HPLC analyses showed that for cellulase treated brans, precipitation of proteins increased phenolic acids and avenanthramides by 14%. Meanwhile, a decreased of 67% and 20% respectively was found for viscozyme and control brans. The effect of protein precipitation on soluble polyphenols is therefore dependent of the carbohydrase, as proteins with different compositions will interact differently with other molecules. Radical scavenging data showed that Cel1 and Vis1 had higher quenching effects on ROO• radicals with activities of 22.1 ± 0.8 and 23.5 ± 1.2 μM Trolox Equivalents/g defatted brans. Meanwhile, CTL2 had the highest HO• radicals inhibition (49.4 ± 2.8% compared to 10.8–32.3% for others. Samples that highly inhibited lipoxygenase (LOX, an enzyme involved in lipid oxidation were Cel1 (23.4 ± 2.3% and CTL1 (18 ± 0.4%.

  19. Separation and purification of both tea seed polysaccharide and saponin from camellia cake extract using macroporous resin.

    Science.gov (United States)

    Yang, Pengjie; Zhou, Mingda; Zhou, Chengyun; Wang, Qian; Zhang, Fangfang; Chen, Jian

    2015-02-01

    A novel method to separate and purify tea seed polysaccharide and tea seed saponin from camellia cake extract by macroporous resin was developed. Among four kinds of resins (AB-8, NKA-9, XDA-6, and D4020) tested, AB-8 macroporous resin possessed optimal separating capacity for the two substances and thus was selected for the separation, in which deionized water was used to elute tea seed polysaccharide, 0.25% NaOH solution to remove the undesired pigments, and 90% ethanol to elute tea seed saponin. Further dynamic adsorption/desorption experiments on AB-8 resin-based column chromatography were conducted to obtain the optimal parameters. Under optimal dynamic adsorption and desorption conditions, 18.7 and 11.8% yield of tea seed polysaccharide and tea seed saponin were obtained with purities of 89.2 and 96.0%, respectively. The developed method provides a potential approach for the large-scale production of tea seed polysaccharide and tea seed saponin from camellia cake. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. A Microwave-Assisted Simultaneous Distillation and Extraction Method for the Separation of Polysaccharides and Essential Oil from the Leaves of Taxus chinensis Var. mairei

    Directory of Open Access Journals (Sweden)

    Chunjian Zhao

    2016-01-01

    Full Text Available An efficient microwave-assisted simultaneous distillation and extraction (MA-SDE method has been developed for the separation of polysaccharides and essential oil from Taxus chinensis var. mairei. The key operating parameters for MA-SDE were optimized by single factor and central composite design experiments, and the optimal conditions were found to include a particle size of 60–80 mesh, liquid/solid ratio of 22.5 mL/g, extraction time of 17.5 min, microwave power of 547 W, and dichloromethane was used as the extraction solvent of the essential oil. The yields obtained for polysaccharides and essential oil under the optimized conditions were 6.39% ± 0.12% and 0.27% ± 0.03%, respectively. The MA-SDE method was also compared with conventional heat reflux extraction (HRE and hydrodistillation extraction (HDE. The MA-SDE method not only allowed for the simultaneous extraction of polysaccharides and essential oil, but also completed the task with a much shorter extraction time of 17.5 min (HRE and HDE required 3 and 6 h, respectively. Furthermore, the MA-SDE method gave increased extraction yields for polysaccharides (1.14-fold higher than HRE and essential oil (1.23-fold higher than HDE. Based on these results, this MA-SDE method represents a rapid and efficient technique for the simultaneous extraction of polysaccharides and essential oil.

  1. Extraction, characterization and antioxidant activities of Se-enriched tea polysaccharides.

    Science.gov (United States)

    Wang, Yuanfeng; Li, Yongfu; Liu, Yangyang; Chen, Xueqing; Wei, Xinlin

    2015-01-01

    Se-polysaccharides from Se-enriched tea leaves were purified by DEAE-sepharose fast flow gel column (2.5×60cm) and three polysaccharide fractions (Se-TPS1, Se-TPS2, and Se-TPS3) were isolated and purified with yields of 6.5, 37.14, and 8.57%, respectively. The average sizes of Se-TPS1 and Se-TPS2 were determined by HPGPC system, with molecular weights of 1.1×10(5) and 2.4×10(5)Da, respectively. Se-TPS3 was a polysaccharide polymer with two peaks with molecular weights of 9.2×10(5) and 2.5×10(5)Da. Monosaccharide components analysis by ion chromatography revealed Se-polysaccharides were acidic polysaccharoses and different from each other in monosaccharide kinds and molar ratio. Elements of Se, C, H, N, S, and 14 kinds of mineral elements were analyzed by AFS, EA, and ICP-AES, respectively. Spectral analysis (IR and UV) indicated Se-polysaccharides were typical glycoproteins. Morphological analyses of the samples were determined by SEM and AFM. In addition, the DPPH and superoxide radicals scavenging activities were also discussed to assess antioxidant activities of the samples, and Se-polysaccharides showed higher antioxidant activities compared to the ordinary polysaccharides.

  2. MICROWAVE- ASSISTED EXTRACTION TECHNOLOGY OF POLYSACCHARIDES FROM GRIFLOLA FRONDOSA%泰山灰树花茵丝体多糖的微波辅助提取

    Institute of Scientific and Technical Information of China (English)

    李晓倩

    2011-01-01

    利用微波辅助提取灰树花菌丝体多糖.以粗多糖的得率为考察指标,以时间、微波功率、液料比为考察因素,通过单因素实验和正交实验,确定微波辅助提取灰树花菌丝体多糖的最佳实验条件;采用硫酸-苯酚法对多糖含量进行了测定.微波辅助提取灰树花多糖正交实验优化结果为:时间6 min,功率:200 W,料液比150:1,多糖得率为13.1%.微波辅助提取灰树花多糖与传统水提取法相比可缩短时间,提高粗糖产率,是一种有前景的多糖辅助提取方法.%To extract the mycelium polysaccharides from Griflola frondosa by the method assisted with microwave. In order to determine the optimal extraction condition, the conditions of polysaccharides extraction including extraction time , microwave power and ratw of water to material were studied by single factor experiment and orthogonal design; The polysaccharide was determined by phenol - sulphoacid method. The experimental results showed that the optimum extraction condition of mycelium polysaccharides from Griflola frondosa was as following: the extraction time is 6min, 200W the miwowave power is the ratio of water to material is, 150:1 the polysaccharides extraction rate was 13.1%. Compared with the traditional water extraction, microwave - assisted extraction of polysaccharide could reduce the time and increase the yield of raw polysacharide, and it was a promising assisted extraction method of polysaccharides.

  3. Study on the Extraction Method of Polysaccharide from Medicago Sativa%紫花苜蓿中多糖的提取方法研究

    Institute of Scientific and Technical Information of China (English)

    刘晓峰; 达哇卓玛; 杨国柱

    2013-01-01

    [Objective] The aim was to study the optimal extraction conditions of polysaccharide from Medicago sativa.[Method] The polysaccharide was extracted from Medicago sativa by using hot water extraction method.Effects of four factors like extraction time,extraction temperature,solid-liquid ratio and extraction times on the extracting rate of polysaccharide extracted from Medicago sativa were studied through single-factor experiment and orthogonal experiment.[Result] The optimal extraction conditions of polysaccharide from Medicago sativa were as followed:the extraction time was 1 h ; the extraction temperature was 95 ℃ ; the solid-liquid ratio was 1 ∶ 30 ; and the extracting time was three times.Under the above conditions,the extracting rate of polysaccharide was up to 6.1%.[Conclusion] The research results provides theoretical basis for the study and production of polysaccharide from Medicago sativa.%[目的]探究提取紫花苜蓿中多糖的最佳工艺条件.[方法]采用热水浸提法提取紫花苜蓿中多糖,并通过单因素试验和正交试验研究了浸提时间、浸提温度、浸提固液比、浸提次数对提取紫花苜蓿中多糖得率的影响.[结果]紫花苜蓿中多糖的最佳提取工艺条件为浸提时间1h、浸提温度95℃、固液比1∶30、浸提3次.在该最佳提取条件下,多糖的得率可达6.1%.[结论]为紫花苜蓿多糖的研究和生产提供了理论依据.

  4. Extraction, preliminary characterization and evaluation of in vitro antitumor and antioxidant activities of polysaccharides from Mentha piperita.

    Science.gov (United States)

    Liu, Xin; Sun, Zhen-Liang; Jia, Ai-Rong; Shi, Ya-Ping; Li, Rui-Hong; Yang, Pei-Ming

    2014-01-01

    This study describes the extraction, preliminary characterization and evaluation of the in vitro antitumor and antioxidant activities of polysaccharides extracted from Mentha piperita (MPP). The optimal parameters for the extraction of MPP were obtained by Box-Behnken experimental design and response surface methodology (RSM) at the ratio of water to raw material of 20, extraction time of 1.5 h and extraction temperature at 80 °C. Chemical composition analysis showed that MPP was mainly composed of glucuronic acid, galacturonic acid, glucose, galactose and arabinose, and the molecular weight of its two major fractions were estimated to be about 2.843 and 1.139 kDa, respectively. In vitro bioactivity experiments showed that MPP not only inhibited the growth of A549 cells but possessed potent inhibitory action against DNA topoisomerase I (topo I), and an appreciative antioxidant action as well. These results indicate that MPP may be useful for developing safe natural health products.

  5. Comparison of the antitumor activity of polysaccharides extracted by boiling water and enzyme assistance from Ganoderma lucidum

    Institute of Scientific and Technical Information of China (English)

    Xu Chunhua; Zhang Chenju; Tian Zhenle; Zheng Huihua; Yu Xiaobing

    2014-01-01

    Polysaccharides are the most important pharmacologically active constituents of Ganoderma lu-cidum. In this work,polysaccharides were extracted from Ganoderma lucidum with boiling water method and enzyme assisted method. The human liver hepatocellular carcinoma cell line HepG2 was used to compare the an-titumor effect of the two kinds of extraction with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bro-mide (MTT) test. Both of these two kinds of Ganoderma lucidum polysaccharides reduced cell viability of can-cer cell HepG2 in a dose and time-dependent manner. At low concentrations,there was no significant difference in the effectiveness of L1 and L2;while at concentrations over 0.8μg/mL,the difference in the effectiveness of L2 in comparison to L1 became significant. At the concentrations of 3.2μg/mL,the cancer cells were almost killed in 2 d.

  6. Structural Characterization and Antioxidant Activities of Polysaccharides Extracted from the Pulp of Elaeagnus angustifolia L.

    OpenAIRE

    Qingqing Chen; Juncheng Chen; Hongtao Du; Qi Li; Jun Chen; Gechao Zhang; Hong Liu; Junru Wang

    2014-01-01

    In this study, two polysaccharides (Elaeagnus angustifolia L. polysaccharide-1 (PEA-1) and PEA-2) were prepared from Elaeagnus angustifolia L. Then, the preliminary structure and antioxidant activities of all the samples were investigated. The results showed that the average molecular weights for PEA-1 and PEA-2 were 9113 and 5020 Da, respectively. And, PEA-1 was mainly composed of rhamnose, xylose, mannose, glucose, and galactose, respectively. The components of PEA-2 were rhamnose, mannose,...

  7. In Vitro Antioxidant Activities of Sulfated Derivatives of Polysaccharides Extracted from Auricularia auricular

    OpenAIRE

    Zhi Zhang; Xue Wang; Lin Yang; Xin Yang; Zhen-Yu Wang; Hua Zhang

    2011-01-01

    In this research, two types of sulfated polysaccharide derivatives were successfully synthesized. Their antioxidant activities were investigated by employing various established in vitro systems. In addition, the degree of sulfation was evaluated using ion-chromatography and IR spectra. The results verify that, when employing scavenging superoxide radical tests, both the sulfation of acid Auricularia auricular polysaccharides (SAAAP) and the sulfation of neutral Auricularia auricular polysacc...

  8. Extract of Reishi polysaccharides induces cytokine expression via TLR4-modulated protein kinase signaling pathways.

    Science.gov (United States)

    Hsu, Hsien-Yeh; Hua, Kuo-Feng; Lin, Chun-Cheng; Lin, Chun-Hung; Hsu, Jason; Wong, Chi-Huey

    2004-11-15

    We have demonstrated that an extract of Ganoderma lucidum (Reishi or Ling-Zhi) polysaccharides (EORP) exerts immunomodulating activities by stimulating the expression of inflammatory cytokines from mouse spleen cells. Interestingly, via responding to LPS in genetic variation of murine macrophage HeNC2 and GG2EE cell lines, and using TLR4 Ab blockage in human blood-derived monocytic macrophages, we have found that the TLR4, but not complement receptor type 3, is a putative receptor of EORP, mediating the consequent immunomodulating events associated with IL-1 gene expression. Based on our studies of reactive oxygen species production, polymyxin B inhibition, and protein tyrosine kinase (PTK) activity, we ruled out the possibility of LPS contamination in EORP. We have found that EORP differentially modulates the protein kinase (PK)-mediated signal transduction pathways associated with inflammatory cytokine IL-1. In human macrophages and murine macrophage J774A.1 cells, EORP was found to up-regulate IL-1 secretion and pro-IL-1 (precursor of IL-1) as well as IL-1-converting enzyme expression. Specifically, EORP rapidly stimulates PTK-mediated phosphorylation, followed by induction of PKs and activation of MAPKs: ERK, JNK, and p38. Using PK inhibitors in the kinase activity assays, Western blot analyses and IL-1 ELISA, we have extensively examined and dissected the role of individual PK in the regulation of pro-IL-1/IL-1. Our findings establish that EORP-mediated signaling pathways are involved in the pro-IL-1/IL-1 regulation: PTK/protein kinase C/MEK1/ERK and PTK/Rac1/p21-activated kinase/p38.

  9. Antitumor activity of polysaccharide extracted from Pleurotus ostreatus mycelia against gastric cancer in vitro and in vivo.

    Science.gov (United States)

    Cao, Xiang-Yu; Liu, Jian-Li; Yang, Wei; Hou, Xiao; Li, Qi-Jiu

    2015-08-01

    The present study aimed to determine the antitumor effects of polysaccharides extracted from Pleurotus ostreatus mycelium on gastric cancer in vitro and in vivo. Polysaccharides were extracted from Pleurotus ostreatus mycelium and an antitumor component, known as Pleurotus ostreatus mycelium polysaccharides 2 (POMP2), with a relative molecular weight of 29 kDa, was then sequentially purified using Sephadex G200 size-exclusion chromatography and diethylaminoethyl-52 cellulose ion-exchange chromatography. The MTT method was used to determine the proliferation of BGC-823 cells treated with POMP2; cell migration assay, colony formation assay and in vivo antitumor tests were used to assess the effect of POMP2 on migration, cell survival and the in vivo tumor formation of BGH-823 cells. Results of the MTT assay indicated that POMP2 had a marked inhibitory effect on the BGC-823 human gastric cancer cell line; when administered at a concentration of 400 mg/l for 72 h, the rate of inhibition was 35.6%. In addition, the colony forming capacity of the BGC-823 cells was significantly reduced following treatment with POMP2. A migration assay indicated that the invasive capabilities of the BGC-823 cells were also significantly inhibited by POMP2. Furthermore, in vivo tests of mice engrafted with BGC-823 cancer cells demonstrated that both tumor weight and volume were markedly reduced following two weeks of treatment with POMP2. The results of the present study suggested that the polysaccharide POMP2 may have a potential application as a natural antitumor treatment for gastric cancer.

  10. Fucans, sulfated polysaccharides extracted from brown seaweeds, inhibit vascular smooth muscle cell proliferation. II. Degradation and molecular weight effect.

    Science.gov (United States)

    Logeart, D; Prigent-Richard, S; Boisson-Vidal, C; Chaubet, F; Durand, P; Jozefonvicz, J; Letourneur, D

    1997-12-01

    Fucan, a sulfated polysaccharide extracted from brown seaweeds, inhibits smooth muscle cell (SMC) proliferation with a higher antiproliferative activity than heparin (Logeart et al., Eur. J. Cell Biol. 74, 1997, this issue). In order to investigate the structure-activity relationship of fucan on SMC growth, we have prepared by size exclusion chromatography fucan fractions of various molecular masses ranging from 5.5 to 556 kDa. Our experiments showed that the antiproliferative activity is dependent on the molecular weight of the polysaccharide. The molecular weight threshold indicated that about 30 saccharidic units on fucan were necessary to give the antiproliferative activity on SMCs. A kinetics study of DNA synthesis using tritiated thymidine uptake was also performed with different molecular weight fucan fractions. Although all tested fractions acted as soon as the cells enter the first cell cycle, the duration and potency of action varied. Moreover, displacement experiments of iodinated fucan revealed that the low molecular fucan fraction interacted weakly with the binding sites. Finally, gel permeation chromatography of internalized radiolabeled heparin and fucans was performed with SMCs. A rapid degradation of internalized heparin was observed, whereas only low molecular weight fucan fractions were partially degraded by SMCs. Together, these results indicate the significance of molecular weight on the antiproliferative activity of fucans on SMCs, and might help to understand their mechanism of action. In addition, the degradation experiments with internalized heparin and fucans ruled out a direct link between polysaccharide degradation and the antiproliferative effect on SMCs.

  11. 麒麟菜中多糖的提取研究%Study on Extraction of Polysaccharides from Eucheuma

    Institute of Scientific and Technical Information of China (English)

    杜云建; 赵玉巧; 黄国军

    2012-01-01

    Hot water extraction of polysaccharides from Eucheuma was studied. The effects of extraction time, extraction temperature, extraction solid-liquid ratio on extraction rate were investigated by single factor experiments. The optimal extraction conditions with single factors were as follows: solid-liquid ratio 1 : 90, extracting time 3 h, extraction temperature 100 ℃. The extraction conditions were optimized by response surface analysis, and the optimized extraction conditions ware as follows: solid-liquid ratio 1 : 95, extracting time 3.7 h, extraction temperature 97.3 ℃, the extraction rate was 31.53%. The optimized extraction conditions were verificated through the experiments; results show the correctness of the model.%采用热水浸提法提取麒麟菜中的多糖。研究了提取温度、提取时间以及固液比对多糖提取率的影响,单因素试验结果表明较佳的提取条件为,时间为3h,提取温度为100℃,提取液固比为90:1,通过响应面优化得到提取的最佳工艺条件为:时间为3.7h,温度为97.3℃,液固比为95:1,提取率为31.5261%,并通过试验对其进行验证,结果证明了所建模型的正确性。

  12. Structural characterization and antioxidant activities of polysaccharides extracted from the pulp of Elaeagnus angustifolia L.

    Science.gov (United States)

    Chen, Qingqing; Chen, Juncheng; Du, Hongtao; Li, Qi; Chen, Jun; Zhang, Gechao; Liu, Hong; Wang, Junru

    2014-06-26

    In this study, two polysaccharides (Elaeagnus angustifolia L. polysaccharide-1 (PEA-1) and PEA-2) were prepared from Elaeagnus angustifolia L. Then, the preliminary structure and antioxidant activities of all the samples were investigated. The results showed that the average molecular weights for PEA-1 and PEA-2 were 9113 and 5020 Da, respectively. And, PEA-1 was mainly composed of rhamnose, xylose, mannose, glucose, and galactose, respectively. The components of PEA-2 were rhamnose, mannose, glucose, and galactose, respectively. Moreover, the Antioxidant assays demonstrated that PEA-1 possessed of strong free radicals scavenging activity and hydroxyl radicals scavenging activities, suggesting that PEA-1 could potentially be used as natural antioxidant.

  13. Bioassay-guided extraction of crude fucose-containing sulphated polysaccharides from Sargassum fusiforme with response surface methodology

    Science.gov (United States)

    Fu, Zhifei; Li, Haihua; Liu, Hongbing; Hu, Shuman; Li, Yueying; Wang, Mengxue; Guan, Huashi

    2016-06-01

    The response surface methodology (RSM) combined with bioassays was employed to optimize the extraction process of crude fucose-containing sulphated polysaccharides (cFCSP) from Sargassum fusiforme. The central composite design (CCD) was used with four variables, five levels, and four responses. The four variables were pH value of hydrochloric acid solution, extraction temperature (°C), ratio of liquid to raw material (mL g-1), and extraction time (h), respectively. Chemical and bioassay indices were used in combination as the response parameters, which included the yield of cFCSP, fucose content, proliferation rate of spleen cells, and lipopolysaccharide-induced proliferation of splenocytes. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis, and examined using the appropriate statistical methods. The best extraction conditions were as follows: the pH value of hydrochloric acid solution was 3.50; the extraction temperature was 100°C; the ratio of liquid to raw material was 15.00 mL g-1 and the extraction time was 2.50 h. The experimental yield was close to the predicted from the model. The extract could promote spleen lymphocyte proliferation, especially the lipopolysaccharide-induced lymphocyte proliferation in vitro, which suggested that its immunomodulatory effect on B lymphocytes. Therefore, cFCSP extracted from S. fusiforme could be utilized as an immunostimulant in functional foods and pharmaceutical industry in future.

  14. Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor

    NARCIS (Netherlands)

    Kozarski, M.; Klaus, A.; Niksic, M.; Vrvic, M.M.; Todorovic, N.; Jakovljevic, D.; Griensven, van L.J.L.D.

    2012-01-01

    Antioxidant activities of polysaccharide extracts of four of the most widely known mushrooms often used in medicinal applications as well as in tea and food, namely Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor, were studied. G. applanatum and L edodes extracts wer

  15. Extraction, preliminary characterization and antioxidant activity of Se-enriched Maitake polysaccharide.

    Science.gov (United States)

    Mao, Guanghua; Zou, Ye; Feng, Weiwei; Wang, Wei; Zhao, Ting; Ye, Changwen; Zhu, Yang; Wu, Xueshan; Yang, Liuqing; Wu, Xiangyang

    2014-01-30

    A Se-enriched Grifola frondosa polysaccharide (Se-GP) was obtained from G. frondosa enriched with Se by spraying an Na2SeO3 solution during fruit body growth using a Box-Behnken design and compared to G. frondosa polysaccharide (GP) for preliminary characterization and determination of the antioxidant activity. Under optimal conditions, polysaccharide yields and both the Se-GP and GP contents do not differ; however, the Se content of Se-GP (17.52 μg/g) was 48.7 times that of GP. Three homogenous Se-GPs (Se-GP11, Se-GP22 and Se-GP33) or GPs (GP11, GP22 and GP33) were obtained via DEAE-52 and Sephacryl S-400 purification. Their molecular weight and polysaccharide content of these compounds were not obviously different, whereas the Se content of Se-GP11, Se-GP22 and Se-GP33 was 9.41, 6.59 and 16.25 times that of GP11, GP22 and GP33, respectively. The antioxidant activity of Se-GP for the DPPH, ABTS and hydroxyl radicals was higher than that of GP and was highest for the hydroxyl radical.

  16. Antioxidant and anti-inflammatory potential of the aqueous extract and polysaccharide fraction from brown marine macroalgae Padina sp. from Gulf of Mannar of Peninsular India

    Institute of Scientific and Technical Information of China (English)

    Kajal Chakraborty

    2013-01-01

    Objective: To evaluate the antioxidant and anti-inflammatory potential of the aqueous extract and polysaccharide fraction from two brown marine macroalga, Padina gymnospora (P.gymnospora ) and Padina tetrastomatica (P. tetrastomatica) harvested from Gulf of Mannar of peninsular India.Methods:The antioxidant activity was evaluated using different in vitro systems, viz., 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2′-azino-bis-3ethylbenzothiozoline-6-sulfonic acid diammonium salt (ABTS), H2O2/ HO. radical scavenging, Fe2+ ion chelating ability, and reducing potential. Folin–Ciocalteu method was used to determine the total phenolic content, and the results were expressed as mg of gallic acid equivalents (GE). Thiobarbituric acid-reactive substance formation inhibition assay was employed to assess the ability of the samples to inhibit lipid oxidation in a model system. COXI and LOXV inhibition assays were employed to assess the anti-inflammatory potential of aqueous extract and polysaccharide fraction.Results:The aqueous extract fraction of P. tetrastomatica realized high total phenolic content (288 mg GE/g), and its activity towards scavenging short-lived radicals (OH. and H2O2) (27.8% and 68.3%, respectively; 0.6 mg/mL) are higher than those registered for Padina gymnospora. Aqueous extract and polysaccharide fractions of P. gymnospora showed higher anti-inflammatory activities against LOXV (56% and 53%, respectively) and COXI (30% and 35%, respectively; 1 mg/mL) enzymes. The correlation studies confirmed that polysaccharides present with the Padina sp. are responsible for their anti-inflammatory potential. IR spectral data of polysaccharide fraction revealed the presence of polysaccharide in alginate form and also confirmed the presence of sulphated polysaccharides as principle bioactive constituents.Conclusions:The study revealed that these seaweeds possess beneficial value as human food or health additives and can be used as a natural green remedy against

  17. Antioxidant and anti-inflammatory potential of the aqueous extract and polysaccharide fraction from brown marine macroalgae Padina sp. from Gulf of Mannar of Peninsular India

    Directory of Open Access Journals (Sweden)

    Nammunayathuputhenkotta Krishnankartha Praveen

    2013-08-01

    Full Text Available Objective: To evaluate the antioxidant and anti-inflammatory potential of the aqueous extract and polysaccharide fraction from two brown marine macroalga, Padina gymnospora (P. gymnospora and Padina tetrastomatica (P. tetrastomatica harvested from Gulf of Mannar of peninsular India. Methods: The antioxidant activity was evaluated using different in vitro systems, viz., 1,1- diphenyl-2-picrylhydrazyl (DPPH, 2, 2′-azino-bis-3ethylbenzothiozoline-6-sulfonic acid diammonium salt (ABTS, H2O2/ HO. radical scavenging, Fe2+ ion chelating ability, and reducing potential. Folin–Ciocalteu method was used to determine the total phenolic content, and the results were expressed as mg of gallic acid equivalents (GE. Thiobarbituric acid-reactive substance formation inhibition assay was employed to assess the ability of the samples to inhibit lipid oxidation in a model system. COXII and LOXV inhibition assays were employed to assess the anti-inflammatory potential of aqueous extract and polysaccharide fraction. Results: The aqueous extract fraction of P. tetrastomatica realized high total phenolic content (288 mg GE/g, and its activity towards scavenging short-lived radicals (OH. and H2O2 (27.8% and 68.3%, respectively; 0.6 mg/mL are higher than those registered for Padina gymnospora. Aqueous extract and polysaccharide fractions of P. gymnospora showed higher anti-inflammatory activities against LOXV (56% and 53%, respectively and COXII (30% and 35%, respectively; 1 mg/mL enzymes. The correlation studies confirmed that polysaccharides present with the Padina sp. are responsible for their anti-inflammatory potential. IR spectral data of polysaccharide fraction revealed the presence of polysaccharide in alginate form and also confirmed the presence of sulphated polysaccharides as principle bioactive constituents. Conclusions: The study revealed that these seaweeds possess beneficial value as human food or health additives and can be used as a natural green

  18. Comparison of gamma ray and electron beam irradiation on extraction yield, morphological and antioxidant properties of polysaccharides from tamarind seed

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Jong-il [Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185 (Korea, Republic of); Kim, Jae-Kyung [Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185 (Korea, Republic of); Graduate school of Food and Biotechnology, Korea University, Seoul 146-701 (Korea, Republic of); Srinivasan, Periasamy; Kim, Jae-Hun [Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185 (Korea, Republic of); Park, Hyun-Jin [Graduate school of Food and Biotechnology, Korea University, Seoul 146-701 (Korea, Republic of); Byun, Myung-Woo [Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185 (Korea, Republic of); Lee, Ju-Woon [Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185 (Korea, Republic of)], E-mail: sjwlee@kaeri.re.kr

    2009-07-15

    Tamarind (Tamarindus indica L) seed polysaccharide (TSP) is of great important due to its various biological activities. The present investigation was carried out to compare extraction yield, morphological characteristics, average molecular weights and antioxidant activities of TSP from gamma- and electron beam (EB)-irradiated tamarind kernel powder. The tamarind kernel powder was irradiated with 0, 5 and 10 kGy by gamma ray (GR) and electron beam, respectively. The extraction yield of TSP was increased significantly by EB and GR irradiation, but there was no significant difference between irradiation types. Morphological studies by scanning electron microscope showed that TSP from GR-irradiated tamarind seed had a fibrous structure, different from that of EB irradiated with a particle structures. The average molecular weight of TSP was decreased by the irradiation, and EB treatment degraded more severely than GR. Superoxide radical scavenging ability and total antioxidant capacity of EB-treated TSP showed higher than those of GR-treated TSP.

  19. Comparison of gamma ray and electron beam irradiation on extraction yield, morphological and antioxidant properties of polysaccharides from tamarind seed

    Science.gov (United States)

    Choi, Jong-il; Kim, Jae-Kyung; Srinivasan, Periasamy; Kim, Jae-Hun; Park, Hyun-Jin; Byun, Myung-Woo; Lee, Ju-Woon

    2009-07-01

    Tamarind ( Tamarindus indica L) seed polysaccharide (TSP) is of great important due to its various biological activities. The present investigation was carried out to compare extraction yield, morphological characteristics, average molecular weights and antioxidant activities of TSP from gamma- and electron beam (EB)-irradiated tamarind kernel powder. The tamarind kernel powder was irradiated with 0, 5 and 10 kGy by gamma ray (GR) and electron beam, respectively. The extraction yield of TSP was increased significantly by EB and GR irradiation, but there was no significant difference between irradiation types. Morphological studies by scanning electron microscope showed that TSP from GR-irradiated tamarind seed had a fibrous structure, different from that of EB irradiated with a particle structures. The average molecular weight of TSP was decreased by the irradiation, and EB treatment degraded more severely than GR. Superoxide radical scavenging ability and total antioxidant capacity of EB-treated TSP showed higher than those of GR-treated TSP.

  20. Structural Characterization and Antioxidant Activities of Polysaccharides Extracted from the Pulp of Elaeagnus angustifolia L.

    Directory of Open Access Journals (Sweden)

    Qingqing Chen

    2014-06-01

    Full Text Available In this study, two polysaccharides (Elaeagnus angustifolia L. polysaccharide-1 (PEA-1 and PEA-2 were prepared from Elaeagnus angustifolia L. Then, the preliminary structure and antioxidant activities of all the samples were investigated. The results showed that the average molecular weights for PEA-1 and PEA-2 were 9113 and 5020 Da, respectively. And, PEA-1 was mainly composed of rhamnose, xylose, mannose, glucose, and galactose, respectively. The components of PEA-2 were rhamnose, mannose, glucose, and galactose, respectively. Moreover, the Antioxidant assays demonstrated that PEA-1 possessed of strong free radicals scavenging activity and hydroxyl radicals scavenging activities, suggesting that PEA-1 could potentially be used as natural antioxidant.

  1. Time of harvest affects the yield of soluble polysaccharides extracted enzymatically from potato pulp

    DEFF Research Database (Denmark)

    Ravn, Helle Christine; Sørensen, Ole Bandsholm; Meyer, Anne S.

    2015-01-01

    potato polysaccharides applying a recently developed enzymatic process using 1.0% (w/w) [enzyme/substrate (E/S)] pectin lyase from Aspergillus nidulans and 1.0% (w/w) [E/S] polygalacturonase from A. aculeatus at 60 °C, 100 mM citric acid, pH 6.0 for 1 min. Seven samples drawn within the potato starch...

  2. In vitro antioxidant properties of flavonoids and polysaccharides extract from tobacco (Nicotiana tabacum L.) leaves.

    Science.gov (United States)

    Ru, Qiao-Mei; Wang, Li-Juan; Li, Wei-Ming; Wang, Jing-Lu; Ding, Yu-Ting

    2012-09-21

    In the present study, antioxidant properties of flavonoids and polysaccharides from tobacco (Nicotiana tabacum L.) leaves were evaluated in several in vitro systems, e.g., scavenging activities on hydroxyl, superoxide anion, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals, and reducing power. Flavonoids showed much better activity than polysaccharides in scavenging activities on free radicals. When compared to the positive control, ascorbic acid, both showed weaker antioxidant potential. However, flavonoids possessed comparable superoxide anion, DPPH and ABTS radical scavenging abilities to ascorbic acid at high concentration (600 μg/mL). Meanwhile, it was found that flavonoids had prominent effects on the reducing power, which was equivalent to ascorbic acid, and was significantly higher than polysaccharides. These results clearly indicate that flavonoids are effective in scavenging free radicals and have the potential to be powerful antioxidants. Thus, tobacco leaves could be considered as a potential source of natural antioxidants for food, pharmaceutical, cosmetics or nutraceutical industries.

  3. 超声波提取紫薯多糖的工艺优化%Optimization of Ultrasonic Extraction Process of Ipomoea batatas ( Linn. ) Lam. Polysaccharide

    Institute of Scientific and Technical Information of China (English)

    姚惠伶; 蒋林彬

    2011-01-01

    [ Objective] To optimize the extraction conditions of polysaccharide from lpomoea batatas ( Linn. ) Lam. [ Method ] Polysaccharide from Ipomoea batatas ( Linn. ) Lam. was extracted by ultrasonic technology,and the extraction conditions were optimized by orthogonal test combined with single factor test. [ Result ] The influencing order of each factor on yield of polysaccharide was determined to be ultrasonic frequency > solid to liquid ratio > extraction temperature > extraction duration. The optimum conditions for extracting polysaccharide from Ipomoea batatas ( Linn. ) Lam. were: solid to liquid ratio of 1: 15, extraction duration of 40 min, extraction temperature of 60 ℃ and ultrasonic frequency of 80 kHz. Under the optimal extracting conditions,extraction rate of polysaccharide from Ipomoea batatas (Linn.) Lam. reached as high as 3.63%.[ Conclusion] Ipomoea batatas ( Linn. ) Lam. polysaccharide extracted by ultrasonic technology was high in yield and short in extraction time. If carried out at low temperature,the extraction was less in extracting solvent and less in cost.%[目的]优化紫薯多糖的提取工艺.[方法]利用超声波技术提取紫薯粗多糖,用单因素试验和正交试验设计相结合的方法获得最佳提取工艺.[结果]影响紫薯多糖得率的主要因素按重要性排序为:超声频率>料液比>提取温度>提取时间;紫薯粗多糖最佳提取工艺条件为:料液比1:15,提取时间40min,提取温度60℃,超声频率80 kHz,此条件下紫薯多糖的提取率为3.63%.[结论]利用超声技术提取紫薯多糖可以提高多糖得率、缩短提取时间、采用较低温度提取、节省提取溶剂,降低提取成本.

  4. 植物叶多糖的提取和生物活性综述%Review on Extraction and Biological Activity of Polysaccharides from Plant Leave

    Institute of Scientific and Technical Information of China (English)

    许春平; 杨琛琛; 郑坚强; 毛多斌

    2014-01-01

    At present, there are many reports about polysaccharides from plant leaf. In this paper, the extraction methods and biological activities of polysaccharides from plant leave are reviewed. The main extraction methods include solvent extraction (water extraction, ethanol extraction, acid extraction, alkali extraction), ultrasonic assisted extraction, microwave assisted extraction, and enzymatic hydrolysis. Leaf polysaccharides posses various biological activities, mainly including anti-oxidant, anti-fatigue, hypoglycemic, hypolipidemic, immune-stimulating and antimicrobial activities, etc.%对各种植物叶多糖的研究有了很多报道,综述了植物叶多糖的提取方法和功能。主要提取方法包括溶剂提取法(水提法、醇提法、酸提法、碱提法)、超声辅助提取法、微波辅助提取法、酶解法;植物叶多糖具有多种生理功能,主要有抗氧化、抗疲劳、降血糖、降血脂、增强免疫活性以及抑菌作用等。

  5. Extraction, Preliminary Characterization and Evaluation of in Vitro Antitumor and Antioxidant Activities of Polysaccharides from Mentha piperita

    Directory of Open Access Journals (Sweden)

    Xin Liu

    2014-09-01

    Full Text Available This study describes the extraction, preliminary characterization and evaluation of the in vitro antitumor and antioxidant activities of polysaccharides extracted from Mentha piperita (MPP. The optimal parameters for the extraction of MPP were obtained by Box-Behnken experimental design and response surface methodology (RSM at the ratio of water to raw material of 20, extraction time of 1.5 h and extraction temperature at 80 °C. Chemical composition analysis showed that MPP was mainly composed of glucuronic acid, galacturonic acid, glucose, galactose and arabinose, and the molecular weight of its two major fractions were estimated to be about 2.843 and 1.139 kDa, respectively. In vitro bioactivity experiments showed that MPP not only inhibited the growth of A549 cells but possessed potent inhibitory action against DNA topoisomerase I (topo I, and an appreciative antioxidant action as well. These results indicate that MPP may be useful for developing safe natural health products.

  6. Extraction optimization, isolation, preliminary structural characterization and antioxidant activities of the cell wall polysaccharides in the petioles and pedicels of Chinese herbal medicine Qian (Euryale ferox Salisb.).

    Science.gov (United States)

    Wu, Chengying; Wang, Xinsheng; Wang, Hong; Shen, Bei; He, Xiaoxiao; Gu, Wei; Wu, Qinan

    2014-03-01

    Cell wall polysaccharides in the petioles and pedicels of Qian (Euryale ferox Salisb.) (EFPP) were extracted using ultrasound-assisted technique. Response surface methodology (RSM) based on Box-Behnken design (BBD) was employed to optimize extraction parameters for the maximum purity of polysaccharides. The results showed that the optimum extraction conditions were extraction temperature of 80 °C, extraction time of 32 min, ultrasonic power of 270W and liquid-to-solid ratio of 40 mL/g. Under the optimal conditions, the experimental purity of polysaccharides was 62.57% ± 1.68%, which was very close to the predicted. The crude EFPP were isolated using DEAE-52 column and four major fractions (EFPP-1, EFPP-2, EFPP-3 and EFPP-4) were obtained. Typical functional groups of polysaccharides were characteristic for EFPP-1, EFPP-3 and EFPP-4 from FT-IR spectrum. Furthermore, the crude EFPP and three fractions (EFPP-1, EFPP-3 and EFPP-4) possessed appreciable in vitro antioxidant effects on α,α-diphenyl-β-picrylhydrazyl (DPPH), 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), hydroxyl radical scavenging and reducing powers. Then, the crude EFPP and EFPP-4 could effective against H2O2-induced injury on HUVEC and VSMC through enhancement of T-AOC, SOD and CAT activities and decrease of MDA content.

  7. 南瓜多糖提取纯化工艺的研究%Study of extraction and purification of pumpkin polysaccharide by freezing method

    Institute of Scientific and Technical Information of China (English)

    吴鹏

    2011-01-01

    为了提高南瓜多糖的提取率,采用冷冻法对南瓜进行前处理,通过正交实验确定了南瓜多糖提取的工艺参数,采用凝胶层析法对南瓜多糖进行了纯化并测定其分子量,采用了红外光谱对南瓜多糖进行了结构分析.实验结果表明,采用冷冻预处理后,南瓜多糖的提取率增加了77%;正交实验确定的南瓜多糖的优化工艺参数为:提取温度65℃,提取时间2 h,料液比1:16;南瓜粗多糖经SephadexG-200凝胶层析柱后分为2个色谱峰,其平均分子量分别约为21 400和57 500;红外光谱分析显示,南瓜多糖为含有环状结构的多糖.%Pumpkin was pretreated by freezing method to improve the extraction rate of pumpkin polysaccharide. Pumpkin polysaccharide extraction technology parameter was determined by orthogonal experiments. Gel chromatography was used to purify pumpkin polysaccharide and determine molecular weight.Infrared spectrometry was used to analyze the pumpkin polysaccharide structure. Experimental results show that the freezing process can effectively improve the extraction rate of pumpkin polysaccharide. The optimum extraction conditions of pumpkin polysaccharide were as follows : extraction time 2 h , ratio of material to liquid 1∶16, extraction temperature 65 ℃ . Pumpkin polysaccharide was separated into two chromatographic peak by SephadexG-200 gel column chromatography, and the average molecular weight were 21 400 and 57 500, respectively. Infrared spectrum analysis showed pumpkin polysaccharide was cyclic structures amylose.

  8. Antiinflammatory and antinociceptive effects in mice of a sulfated polysaccharide fraction extracted from the marine red algae Gracilaria caudata.

    Science.gov (United States)

    Chaves, Luciano de Sousa; Nicolau, Lucas Antonio Duarte; Silva, Renan Oliveira; Barros, Francisco Clark Nogueira; Freitas, Ana Lúcia Ponte; Aragão, Karoline Sabóia; Ribeiro, Ronaldo de Albuquerque; Souza, Marcellus Henrique Loiola Ponte; Barbosa, André Luiz dos Reis; Medeiros, Jand-Venes Rolim

    2013-02-01

    Many algal species contain relatively high concentrations of polysaccharide substances, a number of which have been shown to have anti-inflammatory and/or immunomodulatory activity. In this study, we evaluated the anti-inflammatory and antinociceptive effects in mice of a sulfated polysaccharide fraction (PLS) extracted from the algae Gracilaria caudata. The antiinflammatory activity of PLS was evaluated using several inflammatory agents (carrageenan, dextran, bradykinin, and histamine) to induce paw edema and peritonitis in Swiss mice. Samples of the paw tissue and peritoneal fluid were removed to determine myeloperoxidase (MPO) activity or TNF-α and IL-1β levels, respectively. Mechanical hypernociception was induced by subcutaneous injection of carrageenan into the plantar surface of the paw. Pretreatment of mice by intraperitoneal administration of PLS (2.5, 5, and 10 mg/kg) significantly and dose-dependently reduced carrageenan-induced paw edema (p < 0.05) compared to vehicle-treated mice. Similarly, PLS 10 mg/kg effectively inhibited edema induced by dextran and histamine; however, edema induced by bradykinin was unaffected by PLS. PLS 10 mg/kg inhibited total and differential peritoneal leukocyte counts following carrageenan-induced peritonitis. Furthermore, PLS reduced carrageenan-increased MPO activity in paws and reduced cytokine levels in the peritoneal cavity. Finally PLS pretreatment also reduced hypernociception 3-4 h after carrageenan. We conclude that PLS reduces the inflammatory response and hypernociception in mice by reducing neutrophil migration and cytokines concentration.

  9. Structural Analysis of Water-soluble Polysaccharide PIP1 Extracted from the Cultured Mycelium of Phellinus igniarius

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Water-soluble crude polysaccharide(PIP) was extracted from cultured mycelium of the fungus Phellinus igniarius . After ethanol precipitation and sepharose CL-6B gel filtration, the fraction of PIP1 was obtained, which was shown to be a homogeneous polysaccharide by means of high-performance liquid chromatography. The structure of PIP1 was determined by using several methods. GC analysis indicates that PIP1 is composed of the monosaccharides of glucose, galactose, and mannose. Their molar ratio is 3.70:4.06:1.00. The molar weight was estimated to be 17 kd via HPLC. IR, GC, partial hydrolysis with acid, periodate oxidation, Smith degradation, methylation, and GC-MS analysis were used for the structural analyses of PIP1. The results show that PIP1 has a small quantity of branch structure. The main glycosidic linkage of PIP1 has a β -configuration. The main chain is made up of a large mass of glucose(1→3 ) and few mannose(1→4); the side chain is composed of glucose(1→3) and galactose(1→6); the nonreduced end is composed of galactose and glucose. The side chains are branched at 6-O of glucose(1→3,6) and mannose(1→4,6). On an average, there are three branches among20 residues. It is presumable that the existence of 1,3-linked Glc in the main and side chains is the main reason for its higher antitumor activity.

  10. Hypolipidemic effect of the polysaccharides extracted from pumpkin by cellulase-assisted method on mice.

    Science.gov (United States)

    Zhao, Xin-Hua; Qian, Li; Yin, De-Lu; Zhou, Yi

    2014-03-01

    The fruit of pumpkin (Cucurbita moschata) is one of the most important vegetables in the world. This study was conducted to investigate the hypolipidemic effect of the polysaccharide isolated from pumpkin (PP). Male Sprague-Dawley rats were divided into three groups and treated with diets containing either high fat, PP, or normal fat. Oral administration of PP could significantly decrease the levels of plasma triacylglycerol (TG), total cholesterol (TC), and plasma low-density lipoprotein cholesterol and increase the levels of fecal fat, cholesterol, and plasma high-density lipoprotein cholesterol. Therefore, results suggest that PP had a high hypolipidemic activity and could be explored as a possible agent for hyperlipidemia.

  11. Optimization of ultrasound assisted extraction (UAE of β-d-glucan polysaccharides from Ganoderma lucidum for prospective scale-up

    Directory of Open Access Journals (Sweden)

    Ibrahim Alzorqi

    2017-03-01

    Full Text Available Three levels of three ultrasonic independent variables were optimized to obtain the maximum yield of water-soluble polysaccharides (PS extracted from Ganoderma lucidum using response surface methodology (RSM. Box–Behnken design (BBD was employed to evaluate the effects of ultrasonic variables on the yield of PS. The parameters that were considered for the optimization are ultrasound power (500–700 W, ultrasonic irradiation time (45–65 min and temperature (70–90 °C. The analysis of variance suggested that the response dependent variable of yield of PS could be expressed by a quadratic polynomial model. The optimal theoretical extraction conditions were found to be an ultrasonic power of 590 W, an irradiation time of 58 min and a temperature of 81 °C. Under these conditions the predicted optimal yield was 52.33 mg. Whereas by following the optimized conditions, the yield of PS by experiments was found to be 52.28 mg which is in a very good agreement with the theoretically predicted one. These outcomes indicate the adequacy of quadratic polynomial model to represent the ultrasonic extraction variables within the ranges of investigation for a volume of 0.25 L; and any prospective scale-up may require modifications in the geometry of the extracting vessel due to the non-linear effects of power ultrasound.

  12. Extraction and antioxidant activities assay of polysaccharides from white Hyacinth bean and promoting-growth to probiotics

    Directory of Open Access Journals (Sweden)

    Ni LEI

    2016-12-01

    Full Text Available The extraction parameters of water-soluble polysaccharides (WPs from white hyacinth bean were optimized using single-factor and orthogonal experiment. The antioxidant activities of WPs were presented by assaying three different radicals, 2,2-diphenyl-1-picrylhydrazy radical (DPPH radical, hydroxyl radical and superoxide radical scavenging ability. In addition, the effects of WPs obtained on the growth of three probiotic strains (Lactobacillus acidophilus LA5, Bifidobacterium bifidum BB01 and Lactobacillus bulgaricus LB6 were also determined by measuring the OD and pH value of culture medium. According to the results, the optimum extraction parameters were as follows: the ratio of water to material was 50, extraction time was 2h and the extraction temperature was 95°C. The yield of WPs reached 1.15±0.07% under this condition. In addition, the WPs had different scavenging ability on three radicals (hydroxyl > DPPH > superoxide. And the WPs could promote the growth of LA5, BB01 and LB6.

  13. 祁东黄花菜多糖的提取及含量测定%Extraction and Determination of Polysaccharides from Daylily of QiDong

    Institute of Scientific and Technical Information of China (English)

    周纪东; 李余动

    2015-01-01

    The polysaccharides were extracted in hot water and isolated by ethanol from Daylily of QiDong, then determined. The content of polysaccharides was determined by spectrophotometric method with phenol sulfuric acid as coloring reagent. Based on the results of single factor tests, the optimum parameters of polysaccharides extraction were studied by orthogonal tests. The re-sults showed that the optimal extraction temperature, time, the ratio of liquid to solid and extraction times were 80℃, 3h, 20:1 and 2 times, respectively. Under this extraction method, the average rate of polysaccharides extraction was 28.36%, RSD=2.04%, the average content of polysaccharides was 14.92%, RSD=2.85%, and the average recovery rate was 100.65%, RSD=3.28%.%以祁东黄花菜为原料,采用水提醇沉法提取出黄花菜多糖并进行含量测定。多糖含量测定方法为苯酚-硫酸法。在单因素试验的基础上,通过正交试验确定最佳浸提工艺条件。结果表明,黄花菜多糖最佳浸提条件为液固比20:1,80℃保温3h,浸提2次。按此浸提,多糖平均提取率为28.36%,RSD=2.04%;多糖平均含量为14.92%,RSD=2.85%;平均回收率为100.65%,RSD=3.28%。

  14. Enhancement of human adaptive immune responses by administration of a high-molecular-weight polysaccharide extract from the cyanobacterium Arthrospira platensis

    DEFF Research Database (Denmark)

    Pedersen, Morten Løbner; Walsted, Anette; Larsen, Rune

    2008-01-01

    The effect of consumption of Immulina, a high-molecular-weight polysaccharide extract from the cyanobacterium Arthrospira platensis, on adaptive immune responses was investigated by evaluation of changes in leukocyte responsiveness to two foreign recall antigens, Candida albicans (CA) and tetanus...

  15. Optimization of Microwave Extraction of Polysaccharide from Ranunculus ternatus%猫爪草多糖的微波提取工艺优化

    Institute of Scientific and Technical Information of China (English)

    王明道; 胡虹; 郜峰; 李素振; 陈红歌

    2011-01-01

    The microwave - assisted extraction technique of polysaccharide from Ranunculus ternatus was optimized by response surface analysis method. The results showed that the treatment time of microwave, power of microwave and the ratio of material to water had remarkable effects on the extractive rate of polysaccharide from R. ternatus. The optimum conditions for the microwave -assisted extraction of polysaccharide from R. ternatus were obtained as follows: the power of microwave being 400 W, the treatment time of microwave being 116 s, and the ratio of material to water being 1: 16. Under the optimum conditions, the extractive rate of polysaccharide from R. ternatus was 8.2%. This result also had a good reproducibility.%采用苯酚-硫酸法,通过响应面分析法对猫爪草多糖微波提取工艺进行优选.结果表明料液比、微波功率、处理时间对猫爪草多糖提取率具有显著影响,猫爪草多糖的微波提取最佳工艺为:料液比1∶16,微波功率50%(400 W),处理时间116 s,猫爪草多糖提取率可达8.2%.该优化工艺简单稳定,重现性好,可用于猫爪草多糖的提取.

  16. Studies on biological activity and extracting methods of sulfated polysaccharides%硫酸酯多糖的生物活性及其提取方法

    Institute of Scientific and Technical Information of China (English)

    刘秋凤; 吴成业

    2012-01-01

    本文分析了硫酸酯多糖的立体结构、分子量和硫酸基等因素对其生物活性的影响;比较了硫酸酯多糖的热水浸提、酶法、超声波提取等几种提取方法的提取率,并从抗凝血活性、抗病毒活性、抗肿瘤活性、抗氧化活性、降血糖和降血脂作用等几个方面综述了硫酸酯多糖所具有的生物活性,提出了硫酸酯多糖向海洋生物药源方面开发应用的展望。%This thesis studies such factors as three - dimensional structure, molecular weight and sulfate radical that influence the biological activity of sulfated polysaccharides, compares the extraction rate of sulfated polysaccharides among several extracting methods including hot water diffusion, enzymatic extraction and ultra- sonic extraction and summarizes the biological activities of sulfated polysaccharides in terms of anticoagulation, anti - virus, anti - tumor, antioxidation, hyperglycemic, hypolipidemic and so on. At last, the paper proposes the prospect of the development and application of sulfated polysaccharides in marine biological pharmaceutical industry.

  17. 阿魏菇多糖的分离纯化与结构分析%The Research of the Extraction, Purification, Structure Analysis of Pleurotus Ferulae Polysaccharide

    Institute of Scientific and Technical Information of China (English)

    刘冰; 许程剑; 王月香; 孙然; 牛博楠; 卢士玲; 李应彪

    2014-01-01

    The purpose of this study was to extract and purificate polysaccharide from the Pleurotus ferulae Lenzi , the basic physical and chemical properties of polysaccharide were obtained, and build a solid foundation at the further polysaccharide from the Pleurotus ferulae Lenzi exploration in the structure , relationship between structure and activity in theory. Pleurotus ferulae polysaccharide was prepared by ethanol precipitation of water extract of the Pleurotus Ferulae,that preliminarily extracted by ethanol,followed by removal of proteins,dialysis against water,precipitaion with alcohol and further purification by chromatography on DEAE-cellulose column and sephadex G-100 column. The thin layer chromatography indicated that polysaccharides from pleurotus ferulae were consisted of four monosaccharides, namely glucose, mannose, galactose and fructose. Basic on infrared and electrophoresis to analysis the mushroom ferulae polysaccharide fractions of structure , and it proved that Pleurotus ferulae polysaccharide was a kind of more pure polysaccharides. Finally, through the analysis by the AFM proved that Pleurotus ferulae polysaccharide molecule had highly branched structure, even between the sugar chain formed ring structure.%通过对新疆阿魏菇多糖进行提纯及分析,了解其基本理化性质,在理论上为深入探究阿魏菇多糖的结构以及构效活性奠定了基础。阿魏菇的热水提取液经乙醇沉淀、去蛋白、逆向流水透析、DEAE-Cellulose和Sephadex G-100柱层析得纯品阿魏菇多糖。薄层色谱分析表明阿魏菇总多糖是由半乳糖,葡萄糖,果糖,甘露糖组成。以红外和电泳为基础,分析了阿魏菇多糖组分的结构,表明其是一种较纯的多糖,原子力显微镜分析表明阿魏菇多糖分子具有高度分枝结构。

  18. Extraction Optimization of Water-Extracted Mycelial Polysaccharide from Endophytic Fungus Fusarium oxysporum Dzf17 by Response Surface Methodology

    Directory of Open Access Journals (Sweden)

    Ligang Zhou

    2012-05-01

    Full Text Available Water-extracted mycelial polysaccharide (WPS from the endophytic fungus Fusarium oxysporum Dzf17 isolated from Dioscorea zingiberensis was found to be an efficient elicitor to enhance diosgenin accumulation in D. zingigerensis cultures, and also demonstrated antioxidant activity. In this study, response surface methodology (RSM was employed to optimize the extraction process of WPS from F. oxysporum Dzf17 using Box-Behnken design (BBD. The ranges of the factors investigated were 1–3 h for extraction time (X1, 80–100 °C for extraction temperature (X2, and 20–40 (v/w for ratio of water volume (mL to raw material weight (g (X3. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis. Statistical analysis showed that the polynomial regression model was in good agreement with the experimental results with the determination coefficient (R2 of 0.9978. By solving the regression equation and analyzing the response surface contour plots, the extraction parameters were optimized as 1.7 h for extraction time, 95 °C for extraction temperature, 39 (v/w for ratio of water volume (mL to raw material weight (g, and with 2 extractions. The maximum value (10.862% of WPS yield was obtained when the WPS extraction process was conducted under the optimal conditions.

  19. Study on extraction technology of marigold stem polysaccharides%接骨木茎多糖的提取条件研究

    Institute of Scientific and Technical Information of China (English)

    刘莹; 赵杰

    2011-01-01

    以接骨木茎为原料,采用水浸提法提取,用苯酚-硫酸法测定多糖含量.探讨了浸提温度、pH值、料液比和浸提时间等因素对多糖提取率的影响.结果表明,最优提取条件为:料液比为1∶20(g∶ mL),温度为85℃,提取时间为3h,提取液的pH值为6.0和1次浸提,其提取率为10.21%.%With the marigold stems as raw material,this experiment adopted the method of immersion ex traction to extract polysaccharides, and used the method of phenol-sulfuric acid to determine the content of polysaccharides. The effect of immersion extraction temperature, pH value, ratio of material to liquid and extraction time on the extraction rate of polysaccharide was studied. The optimal condition was as follows; ratio of materials to liquid was 1:20,temperature was 85℃ .extraction time was 3 h,the pH was 6.0 and extracting one times,the extraction rate was 10.21%.

  20. Technical Report for a Study on the Improvement of Extraction Process and Physiological Activities of Polysaccharides from Undaria pinnatifida by Gamma Irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Jong Il; Lee, Ju Won; Kim, Jae Hun; Yoon, Yo Han; Song, Beom Seok; Yoon, Min Chul; Sung, Nak Yun; Lee, Hak Jyung

    2010-06-15

    By reason of Undaria pinnatifida growth is temperature dependent, U. pinnatifida produced in April and May are obtained as the main byproducts and it is normally wasted. The objective of the this study was investigated to the improvement of extraction process and physiological activities of polysaccharides from U. pinnatifida by gamma irradiation. The extraction yield of fucoidan and laminarrin was increased by gamma irradiation, and the molecular weight of extracted polysaccharides was decreased by irradiation. The effect of gamma irradiation on storage of U. pinnatifida was investigated. No viable cells were observed in samples irradiated at 3 and 5 kGy. Finally, fucoidan and laminarin were applied in the pork patty, and it was shown that lower lipid oxidation and positive effect on microbial stability and quality of the pork patty. These results will suggest that radiation technology can be applied for the extraction of functional materials and storages safe of the seaweeds

  1. Optimization of Extracted Polysaccharide from Seed Melon Pulp%籽瓜瓤中多糖提取条件优化

    Institute of Scientific and Technical Information of China (English)

    赵雪平; 李正英; 李艳梅

    2012-01-01

    Choosing seed melon pulp as raw-materials, polysaccharide is extracted from seed melon pulp .The best extraction condition is solid liquid ratio of 100 : 1, temperature of 95 ℃, extraction time of 3.5 h, and extraction once. Under this condition, the polysaccharide content in dry matter of seed melon pulp was 2.01%.%以巴盟籽瓜为原料,测得籽瓜瓤干物质中多糖的含量为2.01%。单因素、正交优化表明,籽瓜瓤中多糖提取的最佳条件:温度为95℃,提取时间为3.5h,料液比为1:100,提取次数为1次。

  2. Study on the Extraction of Polysaccharides from Daylily by Ultrasonic Technique%黄花菜多糖超声波提取技术的研究

    Institute of Scientific and Technical Information of China (English)

    周纪东; 李余动

    2014-01-01

    研究以祁东黄花菜为原料应用超声波技术提取黄花菜多糖的新工艺,在单因素试验的基础上,通过正交试验确定最佳提取工艺条件。结果表明,黄花菜多糖最佳提取条件为超声波功率150W,液固比20:1,提取时间70min,提取2次。按此提取,多糖得率为28.23%,多糖含量为16.74%。与水提醇沉法相比,超声波提取黄花菜多糖的每次提取时间由3h 减少至70min,而多糖含量则提高了9.55%,故该法可作为黄花菜多糖提取的一种优选方法。%A new method of ultrasonic technique was used to extract the polysaccharides from Daylily of QiDong. Based on the results of single factor tests, the optimum parameters of polysaccharides extraction were studied by orthogonal tests. The results showed that the optimal ultrasound power, extracting time, the ratio of liquid to solid and extracting times were 150W, 70min, 20:1 and 2 times, respectively. Under this extraction method, the average rate of polysaccharides extraction was 28.23%, the average content of polysaccharides was 16.74%. Compared with water extraction and alcohol precipitation, ultrasonic extraction time from 3 h was reduced to 70min and the content of polysaccharides was increased 9.55% . The results indicated that the ultrasonic technique is a better method for extracting the polysaccharides in Daylily.

  3. Complex Enzyme-Assisted Extraction, Purification, and Antioxidant Activity of Polysaccharides from the Button Mushroom, Agaricus bisporus (Higher Basidiomycetes).

    Science.gov (United States)

    Yin, Xiulian; You, Qinghong; Zhou, Xinghai

    2015-01-01

    Agaricus bisporus polysaccharides (ABP) were extracted by complex enzyme-assisted extraction methodology. The following were optimal conditions for the extraction of crude ABP: complex enzyme amount, 2.2%; temperature, 62°C; time, 3 h; and pH, 4. Under these conditions, the experimental yield of crude ABP was 6.87%. The crude ABP was purified by diethylaminoethyl-cellulose 52 chromatography and Sephadex G-100 chromatography, and one fraction-namely, ABP-1-was produced. The ABP-1 contained 93.67% carbohydrate, 1.46% protein, and 0.62% uronic acid. The constituent monosaccharides were predominantly glucose, galactose, mannose, and xylose. The antioxidant activities of ABP-1 were investigated by measuring its scavenging ability on 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, its ferric-reducing activity power, and the reducing power assay. At a concentration of 1.2 mg/mL, ABP-1 seemed to possess good free radical scavenging activity, with a scavenging value of about 56%. The results indicate that ABP-1 has good antioxidant activity.

  4. Study on Ultrasonic Extraction Technique of Broccoli Polysaccharide%超声波法提取西兰花多糖的工艺研究

    Institute of Scientific and Technical Information of China (English)

    郎艳婷; 刘波; 张振宇; 郝明月; 苏万平

    2012-01-01

    采用超声波辅助法提取西兰花多糖,通过单因素试验与正交试验对提取工艺进行优化,以期确定西兰花多糖的最佳提取工艺.结果表明,超声波辅助法提取西兰花多糖的最佳工艺参数为:超声时间50min,超声波功率80W,提取温度60℃,料液比1∶50(mL/g),多糖的得率为8.24%.该试验方法简单、易行,可为西兰花多糖的工业化生产提供参考.%To determine the best broccoli polysaccharide extraction process with the ultrasonic assisted-extraction technology. The technique was optimized by a single factor experiments and orthogonal test. The results showed that the optimum extraction parameters of polysaccharides was that ultrasonic time for50 min, 80 W ultrasonic power at 60℃, ratio of liquid feed 1:50 (ml/g), and polysaccharide yield was 8.24%. The test method is simple, easy, and can provide a reference for the industrial production of broccoli polysaccharide.

  5. 玉米皮多糖的提取及抗氧化性研究%Study on extraction of corn peel polysaccharides and its antioxidation

    Institute of Scientific and Technical Information of China (English)

    张贺; 王倩雯; 曹龙奎

    2015-01-01

    Thecorn peel polysaccharides were extracted by using the methodof water extraction and alcohol precipitation and its antioxidation was studied. Three methods,which were salicylic acid method,DPPH method,adjacent benzene three phenolic autoxidation method,were respectively used to determine the abilitiesofcorn peel polysaccharides scavenging hydroxyl free radical,DPPH radical, superoxide anion free radical. The results showed that the extracting ratioof polysaccharides was up to 6.74%. Thecorn peel polysaccharides had different scavenging effectson these radical,and with the increasingofconcentration,theclearance rate gradually enhanced.%利用水提醇沉法提取玉米皮多糖,并对玉米皮多糖抗氧化性进行研究。分别采用水杨酸法、DPPH法、邻苯三酚自氧化法,测定玉米皮多糖清除羟自由基、DPPH·自由基、超氧阴离子自由基的能力。结果表明,玉米皮多糖的提取率为6.74%。玉米皮多糖对该几种自由基均有不同程度清除作用,并且清除率随着浓度的提高而升高。

  6. Magnetic graphene oxide modified by imidazole-based ionic liquids for the magnetic-based solid-phase extraction of polysaccharides from brown alga.

    Science.gov (United States)

    Wang, Xiaoqin; Li, Guizhen; Row, Kyung Ho

    2017-08-01

    Magnetic graphene oxide was modified by four imidazole-based ionic liquids to synthesize materials for the extraction of polysaccharides by magnetic solid-phase extraction. Fucoidan and laminarin were chosen as the representative polysaccharides owing to their excellent pharmaceutical value and availability. Fourier transform infrared spectroscopy, field-emission scanning electron microscopy, and thermogravimetric analysis were applied to characterize the synthesized materials. Single-factor experiments showed that the extraction efficiency of polysaccharides was affected by the amount of ionic liquids for modification, solid-liquid ratio of brown alga and ethanol, the stirring time of brown alga and ionic liquid-modified magnetic graphene oxide materials, and amount of 1-(3-aminopropyl)imidazole chloride modified magnetic graphene oxide materials added to the brown alga sample solution. The results indicated that 1-(3-aminopropyl)imidazole chloride modified magnetic graphene oxide possessed better extraction ability than graphene oxide, magnetic graphene oxide, and other three ionic-liquid-modified magnetic graphene oxide materials. The highest extraction recoveries of fucoidan and laminarin extracted by 1-(3-aminopropyl)imidazole chloride modified magnetic graphene oxide were 93.3 and 87.2%, respectively. In addition, solid materials could be separated and reused easily owing to their magnetic properties. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  7. Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Sri Puji Astuti Wahyuningsih

    2016-03-01

    Full Text Available Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains β-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1 and adding polysaccharide krestin before-after exposure (P3 had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 ± 0.523 M and 1.456 ± 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2 had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 ± 97.96 µM and 953.86 ± 328.16 µM respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016. Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology & Biology Education, 8(1, 12-17.

  8. Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Sri Puji Astuti Wahyuningsih

    2016-03-01

    Full Text Available Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains β-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1 and adding polysaccharide krestin before-after exposure (P3 had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 ± 0.523 M and 1.456 ± 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2 had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 ± 97.96 µM and 953.86 ± 328.16 µM respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016. Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology & Biology Education, 8(1, 12-17.

  9. Review: prospects for the use of extracts and polysaccharides from marine algae to prevent and treat the diseases caused by Helicobacter pylori.

    Science.gov (United States)

    Besednova, Natalya N; Zaporozhets, Tatyana S; Somova, Larisa M; Kuznetsova, Tatyana A

    2015-04-01

    Helicobacter pylori possesses a broad spectrum of pathogenic factors that allow it to survive and colonize the gastric mucosa, and thus, the pathogenetic targets, which have the same diversity, require search for and the development of alternative, effective, and innocuous means for the eradication of H. pylori. In recent years, fucoidans have been extensively studied due to the numerous interesting biological activities, including the anti-adhesive, anti-oxidative, antitoxic, immunomodulatory, anticoagulant, and anti-infection effects. This review summarizes the data on the effects of extracts and sulfated polysaccharides of marine algae, mainly fucoidans, on pathogenic targets in Helicobacter infection. The pathogenetic targets for therapeutic agents after H. pylori infection, such as flagellas, urease, and other enzymes, including adhesins, cytotoxin A (VacA), phospholipase, and L-8, are characterized here. The main target for the sulfated polysaccharides of seaweed is cell receptors of the gastric mucosa. This review presents the published data about the pleiotropic anti-inflammatory effects of polysaccharides on the gastric mucosa. It is known that fucoidan and other sulfated polysaccharides from algae have anti-ulcer effects, prevent the adhesion of H. pylori to, and reduce the formation of biofilm. The authors speculate that the effect of sulfated polysaccharides on the infectious process caused by H. pylori is related to their action on innate and adaptive immunity cells, and also anti-oxidant and antitoxic potential. Presented in the review are materials indicated for the study of extracts and sulfated polysaccharides from seaweed during H. pylori infection, as these compounds are characterized by multimodality actions. Based on the analysis of literary materials in recent years, the authors concluded that fucoidan can be attributed to the generation of new candidates to create drugs intended for the inclusion in the scheme of eradication therapy of

  10. An experimental design approach to the chemical characterisation of pectin polysaccharides extracted from Cucumis melo Inodorus.

    Science.gov (United States)

    Denman, Laura J; Morris, Gordon A

    2015-03-06

    Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive. These functional properties will depend upon extraction conditions. A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus. The results show that the chemical composition is very sensitive to these conditions and that this has a great influence on for example the degree of branching. Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain. The fitting of regression equations relating yield and composition to extraction conditions can therefore lead to tailor-made pectins for specific properties and/or applications.

  11. Box-Behnken design for extraction optimization of crude polysaccharides from Tunisian Phormidium versicolor cyanobacteria (NCC 466): Partial characterization, in vitro antioxidant and antimicrobial activities.

    Science.gov (United States)

    Belhaj, Dalel; Frikha, Donyez; Athmouni, Khaled; Jerbi, Bouthaina; Ahmed, Mohammad Boshir; Bouallagui, Zouhaier; Kallel, Monem; Maalej, Sami; Zhou, John; Ayadi, Habib

    2017-06-09

    In this study, response surface methodology (RSM) based on Box-Behnken design (BBD) was employed to optimize the aqueous extraction of crude polysaccharides from Tunisian cyanobacteria Phormidium versicolor (NCC 466). The optimal extraction conditions with an extraction yield of 21.56±0.92% were as follows: extraction temperature at 81.05°C, extraction time of 3.99h, and water to raw material ratio of 21.52mLg(-1). Crude Phormidium versicolor polysaccharides (CPv-PS) are found to be a hetero-sulfated-anionic polysaccharides that contained carbohydrate (79.37±1.58%), protein (0.45±0.11%), uronic acids (4.37±0.19%) and sulfate (6.83±0.28%). The carbohydrate fraction was composed of arabinose, xylose, ribose, rhamnose, N-acetyl glucosamine, galactose, glucose, mannose, glucuronic acid and saccharose with corresponding mole percentages of 2.41, 14.58, 2.18, 6.23, 7.04, 28.21, 26.04, 3.02, 0.86 and 5.07, respectively. Evaluation of the antioxidant activity in vitro suggested that CPv-PS strongly scavenged radicals, prevented bleaching of β-carotene and reduced activity. Furthermore, the CPv-PS exhibited effective antimicrobial properties. Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.

  12. Effect of polysaccharides extract of rhizoma atractylodis macrocephalae on thymus, spleen and cardiac indexes, caspase-3 activity ratio, Smac/DIABLO and HtrA2/Omi protein and mRNA expression levels in aged rats.

    Science.gov (United States)

    Guo, Ling; Sun, Yong Le; Wang, Ai Hong; Xu, Chong En; Zhang, Meng Yuan

    2012-10-01

    This study was designed to determine the possible protective effect of polysaccharides extract of rhizoma atractylodis macrocephalae on heart function in aged rats. Polysaccharides extract of rhizoma atractylodis macrocephalae was administered to aged rats. Results showed that thymus, spleen and cardiac indexs were significantly increased, whereas caspase-3 activity ratio, Smac/DIABLO and HtrA2/Omi protein expression, Smac/DIABLO and HtrA2/Omi mRNA expression levels were markedly reduced. It can be concluded that polysaccharides extract of rhizoma atractylodis macrocephalae may enhance immunity and improve heart function in aged rats.

  13. The antioxidative and anti-aging effects of acidic- and alkalic-extractable mycelium polysaccharides by Agrocybe aegerita (Brig.) Sing.

    Science.gov (United States)

    Jing, Huijuan; Li, Juan; Zhang, Jianjun; Wang, Wenshuai; Li, Shangshang; Ren, Zhenzhen; Gao, Zheng; Song, Xinling; Wang, Xiuxiu; Jia, Le

    2017-08-26

    Two extractable polysaccharides of Ac-MPS and Al-MPS were extracted from the mycelium of Agrocybe aegerita (Brig.) Sing and the in vitro antioxidant activities and in vivo anti-aging activities in a d-gal-induced aging mice were investigated in the present study. The in vitro antioxidant analysis showed that Ac-MPS showed superior scavenging effects on hydroxyl of 68.62±2.59% and DPPH of 85.19±3.26% at the concentration of 4500μg/mL. Biochemical indices and antioxidant analysis in vivo showed that Ac-MPS had better effects in raising hepatic activities of SOD, CAT, GPx and T-AOC, inhibiting hepatic contents of LPO and MDA, as well as improving serum lipid levels of LDL-C, HDL-C, LDL-C/HDL-C, TG and TC. Furthermore, HYP measurements suggested that skin collagen in aging mice could be maintained. This study suggested that both Ac-MPS and Al-MPS could be used as potential natural agents or dietary supplements in attenuating aging and its complications. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Chemically-modified polysaccharide extract derived from Leucaena leucocephala alters Raw 264.7 murine macrophage functions.

    Science.gov (United States)

    Gamal-Eldeen, Amira M; Amer, Hassan; Helmy, Wafaa A; Talaat, Roba M; Ragab, Halla

    2007-06-01

    In this study, a chemical modification of the polysaccharides extract (E) derived from Leucaena leucocephala seeds was performed to prepare C-glycosidic 2-propanol derivative (PE), and its sulphated derivative (SPE). This study aimed to characterize immunomodulatory activities of the original extract and its derivatives by exploring their effects on Raw macrophage 264.7 functions and their antioxidant activity. Our results indicated that PE was an effective radical scavenger to hydroxyl, peroxyl, and superoxide anion radicals, and SPE was a peroxyl radical scavenger. PE and SPE were found to influence the macrophage functions. Both of PE and SPE enhanced the macrophage proliferation and phagocytosis of FITC-zymosan; PE inhibited nitric oxide (NO) generation and tumor necrosis factor-alpha (TNF-alpha) secretion in lipopolysaccharide (LPS)-stimulated Raw macrophage 264.7. In contrast, SPE over-induced NO generation and TNF-alpha secretion. Moreover, PE strongly inhibited the binding affinity of FITC-LPS to Raw 264.7, as indicated by flow cytometry analysis. These findings revealed that PE may act as a potent anti-inflammatory agent; however SPE may act as an inducer of macrophage functions against pathogens.

  15. Effects of the polysaccharides extracted from Ganoderma lucidum on chemotherapy-related fatigue in mice.

    Science.gov (United States)

    Ouyang, Ming-Zi; Lin, Li-Zhu; Lv, Wen-Jiao; Zuo, Qian; Lv, Zhuo; Guan, Jie-Shan; Wang, Shu-Tang; Sun, Ling-Ling; Chen, Han-Rui; Xiao, Zhi-Wei

    2016-10-01

    The weight-loaded swimming capability, tumor growth, survival time and biochemical markers of Ganoderma lucidum polysaccharides (GLPs) in a chemotherapy-related fatigue mouse model were tested in the present study. The results showed that the middle-dose GLPs (GLP-M) and the high-dose GLPs (GLP-H) could increase the exhausting swimming time, which was observed to decrease in the cisplatin control group(PCG) and the tumor control group (TCG).The GLP-M and the GLP-H had reduced serum levels of tumor necrosis factor-αand interleukin-6, which were up-regulated by cisplatin. Cisplatin and the presence of tumor significantly enhanced the malondialdehyde (MDA) content and inhibited the activity of superoxide dismutase (SOD) in the muscle. Administration of GLPs at a high dose decreased the levels of MDA and up-regulated the SOD activity. The high-dose GLPs+cisplatin group presented a decreased tendency of tumor volume and a lower tumor weight compared with PCG. Moreover, the mice in the GLP-M and GLP-H groups had longer survival times compared with the mice in the TCG and PCG.The levels of creatinine and serum blood urea nitrogen, which are up-regulated by cisplatin, were significantly reduced by GLP-M and GLP-H. Therefore, these results suggest that GLPs might improve chemotherapy-related fatigue via regulation of inflammatory responses, oxidative stress and reduction of nephrotoxicity.

  16. Chemical Characterization and Antitumor Activities of Polysaccharide Extracted from Ganoderma lucidum

    Directory of Open Access Journals (Sweden)

    Zengenni Liang

    2014-05-01

    Full Text Available Ganoderma lucidum polysaccharide (GLP is a biologically active substance reported to possess anti-tumor ability. Nonetheless, the mechanisms of GLP-stimulated apoptosis are still unclear. This study aims to determine the inhibitory and apoptosis-inducing effects of GLP on HCT-116 cells. We found that GLP reduced cell viability on HCT-116 cells in a time- and dose-dependent manner, which in turn, induced cell apoptosis. The observed apoptosis was characterized by morphological changes, DNA fragmentation, mitochondrial membrane potential decrease, S phase population increase, and caspase-3 and -9 activation. Furthermore, inhibition of c-Jun N-terminal kinase (JNK by SP600125 led to a dramatic decrease of the GLP-induced apoptosis. Western blot analysis unveiled that GLP up-regulated the expression of Bax/Bcl-2, caspase-3 and poly (ADP-ribose polymerase (PARP. These results demonstrate that apoptosis stimulated by GLP in human colorectal cancer cells is associated with activation of mitochondrial and mitogen-activated protein kinase (MAPK pathways.

  17. Study on extraction and purification technology of Nicandra polysaccharide%假酸浆多糖提取纯化工艺的研究

    Institute of Scientific and Technical Information of China (English)

    姜盼; 王遂; 齐欣欣

    2015-01-01

    以假酸浆籽为原料,采用水提法、超声波提取法、微波提取法提取假酸浆多糖,经对各提取过程比较分析可知水提法为最优提取方法,提取条件为料液比1∶55、温度80℃、时间3 h,提取率为6.18%.将水提法得到的假酸浆多糖浓缩液通过醇沉、脱蛋白、脱色等工艺进行纯化,确定了最佳的提取纯化工艺.在最佳的条件下,醇沉后多糖的量可达80.5%,蛋白质脱除率可达70.8%,脱色率可达91.0%.%Nicandra polysaccharide was extracted from the seed of Nicandra physaloides ( L.) Gaertn by the method of water extraction, ultrasonic extraction, microwave extraction, respectively.Through comparative analysis on the extraction process, water extraction achie-ving 6.18%extraction rate was determined to be the optimal method, in which the ratio of material to liquid was 1∶55 , the extraction temperature was 80 ℃, and the extraction time was 3 h.The treatments of ethanol precipitation, deproteinization and decolorization were carried out for purifying to form the final polysaccharide.After the ethanol precipitation, the content of polysaccharide reached 80.5%, the protein removal rate reached 70.8%and the decolorization rate reached 91.0%.

  18. Immune-stimulatory and anti-inflammatory activities of Curcuma longa extract and its polysaccharide fraction

    OpenAIRE

    Chandrasekaran, Chinampudur V.; Kannan Sundarajan; Edwin, Jothie R.; Giligar M Gururaja; Deepak Mundkinajeddu; Amit Agarwal

    2013-01-01

    Background: While curcuminoids have been reported to possess diverse biological activities, the anti-inflammatory activity of polar extracts (devoid of curcuminoids) of Curcuma longa (C. longa) has seldom been studied. In this study, we have investigated immune-stimulatory and anti-inflammatory activities of an aqueous based extract of C. longa (NR-INF-02) and its fractions in presence and absence of mitogens. Materials and Methods: Effects of NR-INF-02 (Turmacin TM , Natural Remedies Pvt. Lt...

  19. Ultrasound assisted pectic polysaccharide extraction and its characterization from waste heads of Helianthus annus.

    Science.gov (United States)

    Ponmurugan, K; Al-Dhabi, Naif Abdullah; Maran, J Prakash; Karthikeyan, K; Moothy, I Ganesh; Sivarajasekar, N; Manoj, J Jony Blessing

    2017-10-01

    The main aim of this current work is to extract pectin from waste heads of Helianthus annus by ultrasound and optimize the process variables (ultrasound power (USP), pH, time of sonication (TS) and ratio of solid to liquid (RSL) on maximal recovery of pectin using central composite statistical experimental design. In addition to that, extracted pectin at optimal condition was characterized and compared with commercial pectin. The optimal extraction process condition was USP of 375w, pH of 3.2, TS of 32min and RSL of 1:15g/ml. Mean experimental pectin yield of 8.89±0.024% was well accord with predicted pectin yield (8.91%). Analysis of chemical composition and Fourier transform infrared spectroscopy of extracted pectin did not show any significant difference with commercial pectin. XRD analysis illustrated a similar crystalline profile in both extracted and commercial pectin. Morphological analysis was performed on fresh and extracted samples using scanning electron microscopy. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Highly virulent strains of Pseudomonas solanacearum that are defective in extracellular-polysaccharide production

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Peilin; Iwata, Michiaki; Sequeira, L. (Dept. of Agriculture (USA)); Leong, S. (Univ. of Wisconsin, Madison (USA))

    1990-07-01

    Extracellular polysaccharide (EPS) has long been regarded as one of the mos important factors involved in wilting of plants by Pseudomonas solanacearum. By means of transposon Tn5 mutagensis, the authors have isolated a class of mutants that have an afluidal colony morphology but retain the ability to cause severe wilting and death of tobacco plants. One such mutant, KD700, was studied in detail. By marker exchange mutagenesis, the altered colony morphology was shown to be the result of a single Tn5 insertion in a 14.3-kilobase EcoRI fragment. This defect could be corrected by introducing a homologous clone from a cosmid library of the wild-type, parental strain K60. The Tn5-containing fragment was introduced into other P. solanacearum wild-type strains by marker exchange, and these altered strains had the same afluidal phenotype as KD700. N-Acetylgalactosamine (GalNac), the major constituent of EPS of all wild-type strains of P. solanacearum, was not detected by gas chromatography-mass spectrometry analysis of vascular fluids from wilting plants infected by KD700. In contrast, GalNac was readily detected in similar fluids of plants infected by K60. Polysaccharides extracted from culture filtrates of KD700 contained approximately one-fifth of the GalNac present in polysaccharides from K60. No differences in growth rates in culture or in planta between the mutant and the parental strains were observed. Since strains that are deficient in EPS production can remain highly virulent to tobacco, they conclude that EPS, or at least its GalNac-containing component, may not be required for disease development by P. solanacearum.

  1. A study of Lycium barbarum polysaccharides (LBP) extraction technology and its anti-aging effect.

    Science.gov (United States)

    Yi, Ran; Liu, Xiao-Min; Dong, Qi

    2013-01-01

    The objective of the study was to optimise the LBP extraction technology and to study the anti-aging effect of LBP by establishing D-gal aging mouse model. Orthogonal design was used to study the extraction technology. The experimental aging mouse model was formed by continuous injection of D-gal, and the anti-aging capacity of LBP was tested using measuring MDA, CAT and GSH-px contents and SOD activity in blood and SOD, MDA and Hyp levels in skin. The results showed that the optimum LBP extraction option determined by the orthogonal design is as follows: solid-liquid ratio of 1:30, extraction for 2 times, 90 min each time, and power is 100 kHz. Thus, LBP can increase SOD, CAT and GSH-px levels in blood and reduce MDA level. It can also improve skin SOD activity, reduce skin MDA content, and increase Hyp content. We concluded that the extraction method established in this experiment is easy and feasible, and the yield of LBP is high, apparently showing that LBP has the potential of delaying senility in D-gal induced mice.

  2. Application of microwave techniques in the extraction of polysaccharides from daylily%微波技术在黄花菜多糖提取中的应用

    Institute of Scientific and Technical Information of China (English)

    周纪东; 李余动

    2015-01-01

    研究应用微波技术以黄花菜为原料提取黄花菜多糖的新工艺。在单因素实验的基础上,通过正交实验确定最佳提取工艺参数。结果表明,黄花菜多糖最佳提取工艺参数为微波功率490 W,提取时间100 s,液固比25∶1,提取2次。按此工艺参数提取,多糖平均得率为31.65%,RSD=2.21%(n=3);多糖平均含量为18.89%,RSD=2.63%(n=3);平均回收率为97.92%,RSD=3.13%(n=6)。与水提醇沉法相比,应用微波技术提取黄花菜多糖,每次提取的时间由3 h减少到100 s,而多糖含量提高了23.63%。%A new method of microwave technique was used to extract the polysaccharides from Daylily.Based on the results of single factor tests,the optimum parameters of polysaccharides extraction were studied by orthogonal tests.The results showed that the optimal microwave power,the time for extraction the ratio of liquid to solid and times of extraction,were 490W,100s,25 ∶1 and 2 times,respectively.With the techniques,the average rate of polysaccharides extraction was 31.65%,RSD=2.21%(n=3),the average content of polysaccharides 18.89%, RSD=2.63%(n=3),and the average recovery rate 97.92%,RSD=3.13%(n=6).Compared with water ex-traction and alcohol precipitation,the time for extraction with microwave was reduced from 3 hours to 100 s and the content of polysaccharides was increased 23 .63%.

  3. Activated charcoal-mediated RNA extraction method for Azadirachta indica and plants highly rich in polyphenolics, polysaccharides and other complex secondary compounds

    OpenAIRE

    Rajakani, Raja; Narnoliya, Lokesh; Sangwan, Neelam Singh; Sangwan, Rajender Singh; Gupta, Vikrant

    2013-01-01

    Background High quality RNA is a primary requisite for numerous molecular biological applications but is difficult to isolate from several plants rich in polysaccharides, polyphenolics and other secondary metabolites. These compounds either bind with nucleic acids or often co-precipitate at the final step and many times cannot be removed by conventional methods and kits. Addition of vinyl-pyrollidone polymers in extraction buffer efficiently removes polyphenolics to some extent, but, it faile...

  4. 川芎多糖提取工艺的研究%Study on the Process of Polysaccharide Extract from Ligusticum chuanxiong Hort

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

      以川芎为原料,采用水提醇沉法对川芎多糖的提取工艺进行研究,通过单因素试验及正交试验优化的最佳提取工艺条件为:物料粒径60目,提取温度60℃,提取时间1.5 h,料液比1∶14,提取2次,川芎多糖提取率可达3.72%。%Taking Ligusticum chuanxiong Hort as raw material, the extraction process of polysaccharide from Ligusticum chuanxiong Hort was studied by using water extraction and alcohol precipitation method. Through the single factor experiment and orthogonal test, the optimum extraction condition was as follows: particle size was 60 mesh, extraction temperature was 60 ℃, extraction time was 1.5 h, the ratio of material to liquid was 1∶14, extracting for 2 times, the extraction rate of polysaccharide from Ligusticum chuanxiong Hort could up to 3.72%.

  5. 姜黄多糖提取工艺优化研究%Study on the Optimal Extraction Technology of Polysaccharide in Curcuma longa L

    Institute of Scientific and Technical Information of China (English)

    杨海玲; 黄文男; 林婕; 许丹妮

    2015-01-01

    探讨姜黄多糖提取最佳工艺。采用单因素与正交实验设计法,对料液比、粉碎目数、提取温度、提取时间4个因素进行考查。结果姜黄多糖的最佳工艺条件为:100目粉末在料液比为1∶40(g/mL)条件下,100℃水浴加热提取4 h。在该条件下,姜黄多糖更加完全,提取率为13.86%。%This experiment was to study the optimal extraction technology of polysaccharide in Curcuma longa L.. The conditions of extraction were used on single factors and orthogonal experiment. The particle sieve size of powders,material to solvent ,extraction temperature and hours was examined. The results showed that the best water extraction technology were as follows:100 particle sieve size of powders,material to solvent 1∶40(g/mL), 100 ℃ extraction temperature and for 4 h .In this condition ,the polysaccharide in Curcuma longa L.could be extracted more complete and the ratio on extraction was 13.86%.

  6. Microwave-assisted extraction of pectic polysaccharide from waste mango peel.

    Science.gov (United States)

    Maran, J Prakash; Swathi, K; Jeevitha, P; Jayalakshmi, J; Ashvini, G

    2015-06-05

    This present study investigates the extraction characteristics and optimal parameters of the microwave-assisted extraction of pectin from waste mango peel (WMP). Microwave power, pH, time and solid-liquid ratio were selected as the extraction parameters and was studied by using Box-Behnken response surface design. The experimental data was analyzed by least square regression analysis method and a second order polynomial model was constructed for response from the experimental data. The constructed model was adequate to explain the relationships between independent variables and response. All studied factors had great influence on the yield of pectin by individually and interactively. The optimum microwave assisted extraction conditions for the highest pectin yield (28.86%) from WMP was found to be: microwave power of 413W, pH of 2.7, time of 134s and solid-liquid ratio of 1:18g/ml. The experimental value was well correlated with predicted value at the optimal condition.

  7. Effect of acemannan, an extracted polysaccharide from Aloe vera, on BMSCs proliferation, differentiation, extracellular matrix synthesis, mineralization, and bone formation in a tooth extraction model.

    Science.gov (United States)

    Boonyagul, Sani; Banlunara, Wijit; Sangvanich, Polkit; Thunyakitpisal, Pasutha

    2014-07-01

    Aloe vera is a traditional wound healing medicine. We hypothesized acemannan, a polysaccharide extracted from Aloe vera gel, could affect bone formation. Primary rat bone marrow stromal cells (BMSCs) were treated with various concentrations of acemannan. New DNA synthesis, VEGF, BMP-2, alkaline phosphatase activity, bone sialoprotein, osteopontin expression, and mineralization were determined by [(3)H] thymidine incorporation assay, ELISA, biochemical assay, western blotting, and Alizarin Red staining, respectively. In an animal study, mandibular right incisors of male Sprague-Dawley rats were extracted and an acemannan treated sponge was placed in the socket. After 1, 2, and 4 weeks, the mandibles were dissected. Bone formation was evaluated by dual-energy X-ray absorptiometry and histopathological examination. The in vitro results revealed acemannan significantly increased BMSC proliferation, VEGF, BMP-2, alkaline phosphatase activity, bone sialoprotein and osteopontin expression, and mineralization. In-vivo results showed acemannan-treated groups had higher bone mineral density and faster bone healing compared with untreated controls. A substantial ingrowth of bone trabeculae was observed in acemannan-treated groups. These data suggest acemannan could function as a bioactive molecule inducing bone formation by stimulating BMSCs proliferation, differentiation into osteoblasts, and extracellular matrix synthesis. Acemannan could be a candidate natural biomaterial for bone regeneration.

  8. Extraction optimization of polysaccharides from Chinese rice wine from the Shaoxing region and evaluation of its immunity activities.

    Science.gov (United States)

    Shen, Chi; Mao, Jian; Chen, Yongquan; Meng, Xiangyong; Ji, Zhongwei

    2015-08-15

    Chinese rice wine is well known for its unique flavor and high nutritional value. It is of interest to investigate the functional components of Chinese rice wine and their health benefits. Response surface design of three factors - pH, ethanol concentration and precipitation time - at three levels was utilized to optimize the extraction of Chinese rice wine polysaccharide (CRWP). The results indicated that the CRWP yield was 77.287% at the optimal levels for pH 8.4, ethanol concentration 88% and precipitation time 23 h. In addition, immune activity of CRWP was investigated by measuring body weight, spleen index and thymus index. Furthermore, immunity activity of CRWP was investigated by measuring lymphocyte proliferation, phagocytic index and phagocytic percentage of immunosuppressed mice. Compared with the control mice and model mice, it was found that CRWP has beneficial immune activities in vivo. These findings indicate that CRWP has immune activities in vivo by modulating the immune response, and implies full development and utilization of the nutritional value of Chinese rice wine. However, further work will be conducted in the future to elucidate the structure-bioactivity relationship for CRWP. © 2014 Society of Chemical Industry.

  9. Blasting extrusion processing: the increase of soluble dietary fiber content and extraction of soluble-fiber polysaccharides from wheat bran.

    Science.gov (United States)

    Yan, Xiaoguang; Ye, Ran; Chen, Ye

    2015-08-01

    In this study, soluble dietary fiber (SDF) content of wheat bran was significantly increased from 9.82 ± 0.16 (w/w, %) to 16.72 ± 0.28 (w/w, %) by a novel blasting extrusion processing with enhanced water retention capacity and the swelling capacity. In addition, a water-soluble polysaccharide (WBP) was isolated and extracted from extruded SDF. WBP was successfully purified from SDF by column chromatography systems with the average molecular weight (Mw) of 4.7 × 10(4)Da, containing arabinose, xylose, glucose, and galactose. With the molar ratio of 0.76:0.99:1.00:0.12. Our results suggest that WBP owned 1 → 2, 1 → 3, 1 → 2, 6 and 1 → 4, 1 → 4, 6 glycosidic bonds in the absence of 1 →, 1 → 6 glycosidic bonds. In vitro antioxidant assays (DPPH, ABTS+ radical scavenging capacities, and ferric ion reducing capacity) demonstrated that WBP possesses good antioxidant capacity, and it could be potentially used as a natural antioxidant for use in functional food, cosmetic and pharmaceutical industries.

  10. Algal Production of Extra- and Intra-Cellular Polysaccharides as an Adaptive Response to the Toxin Crude Extract of Microcystis Aeruginosa

    Directory of Open Access Journals (Sweden)

    Mostafa Mohamed El-Sheekh

    2012-11-01

    Full Text Available This is an investigation concerned with studying the possible adaptive response of four different unicellular algae, Anabaena PCC 7120, Oscillatoria angustissima, Scendesmus obliquus and Chlorella vulgaris, to the toxin of Microcystis aeruginosa (Kützing. Theeffects of four different concentrations, 25, 50, 100 and 200 μg mL-1 of microcystins crude extract of M. aeruginosa, on both intra and extra-cellular polysaccharide levels, in log phase,of the four tested algae were studied. The obtained results showed differential increase in the production levels for both intra and extra-cellular polysaccharides by the tested algae,compared with the control. S. obliquus and C. vulgaris showed a resistance to crude toxinhigher than Anabaena PCC 7120 and O. angustissima. The highly production of polysaccharides by green algal species under this toxic stress indicated the involvement of these polysaccharides in protecting the algal cells against toxic species and, reflect thebiological behavior of particular algal species to the environmental stresses.

  11. Algal production of extra and intra-cellular polysaccharides as an adaptive response to the toxin crude extract of Microcystis aeruginosa

    Directory of Open Access Journals (Sweden)

    El-Sheekh Mostafa

    2012-11-01

    Full Text Available Abstract This is an investigation concerned with studying the possible adaptive response of four different unicellular algae, Anabaena PCC 7120, Oscillatoria angustissima, Scendesmus obliquus and Chlorella vulgaris, to the toxin of Microcystis aeruginosa (Kützing. The effects of four different concentrations, 25, 50, 100 and 200 μg mL-1 of microcystins crude extract of M. aeruginosa, on both intra and extra-cellular polysaccharide levels, in log phase, of the four tested algae were studied. The obtained results showed differential increase in the production levels for both intra and extra-cellular polysaccharides by the tested algae, compared with the control. S. obliquus and C. vulgaris showed a resistance to crude toxin higher than Anabaena PCC 7120 and O. angustissima. The highly production of polysaccharides by green algal species under this toxic stress indicated the involvement of these polysaccharides in protecting the algal cells against toxic species and, reflect the biological behavior of particular algal species to the environmental stresses.

  12. Extraction and structural properties of Acanthophora muscoides (Rhodophyceae extracellular matrix sulfated polysaccharides and their effects on coagulation

    Directory of Open Access Journals (Sweden)

    José Ariévilo Gurgel Rodrigues

    2016-06-01

    Full Text Available Acanthophora muscoides (Rhodophyta contains structurally heterogeneous sulfated polysaccharides (Am-SPs with pharmacological importance; however, its matrix SPs composition has not been still extensively investigated. This study sequentially extracted and compared the structural features and the in vitro anticoagulant effects of the Am-SPs. Papain-extraction sequence yielded Am.E-1, Am.E-2 and Am.E-3 containing differences among the relative proportions of sulfate (26.18-33% and hexoses (42.02-60.67% based on chemical analyses. One- (1H and two-dimensions (1H/13C nuclear magnetic resonance experiments showed very complex Am-SPs composed of alternating 4-linked-α-galactopyranosyl units and 3-linked-β-galactopyranosyl units presenting variable sulfation, CH3 substitutions and3,6-anhydro-α-L-galactose units and pyruvated-D-galactose residues, respectively, typical of agarocolloids. Different chromatographic profiles (DEAE-cellulose were observed, with fractions (Am I, Am II and Am III eluted with 0.5, 0.75 and/or 1 M of NaCl, respectively revealing charge density patterns and distinct mobility to heparin by agarose-electrophoresis and, when analyzed by polyacrylamide-electrophoresis, a dispersive migration and similar mobility as chondroitin-6-sulfate for Am I fractions were noted. Regarding the activated partial thromboplastin time test, fractions had no virtually anticoagulation (1.47→3.07 IU mg-1 in comparison with 193 IU mg-1 heparin. Therefore, Am-SPs show significantly lower anticoagulation than heparin.

  13. 无花果多糖提取工艺及其功能研究%Optimization of polysaccharide extraction process from Ficus carica and its function

    Institute of Scientific and Technical Information of China (English)

    叶文斌

    2016-01-01

    Obj ective]To optimize the technology of polysaccharides extration from Ficus carica,and study its health to alloxan-induced diabetic rats function.[Method]Extracting process of polysaccharides from Ficus carica were optimized by response surface methodology and enzymolysis,and investigating the effects of polysaccharides on glucose and lipidemia and acute toxicity in alloxan-induced diabetic rats.[Re-sult]The optimal extraction conditions were hydrolysis temperature of 50.36 ℃,hydrolysis concentration of 0.06 g ,extraction pH of 4.73 and extracting three times.Under the conditions,the predicted and proved value of polysaccharides extracted were 34.47%and 34.13%,respectively.If polysaccharides were contin-uously intragastric administration for 28 days ,the polysaccharides could significantly reduce blood glucose adrenaline induced hyperglycemia in normal rats and could decrease the levels of FBG ,HbAlc,TC and TG in serum in alloxan-induced diabetic rats,and increase the concentrations of HDL significantly.[Conclu-sion]The optimal process parameters for extracting polysaccharides from Ficus carica was obtained ,and the polysaccharides could significantly decrease the blood glucose and improve the disorder of lipid metabo-lism in alloxan-induced diabetic rats.%【目的】优化无花果多糖提取工艺,研究其生物功能.【方法】采用响应面与酶法联用优化无花果(Ficus carica)多糖的提取工艺,测定无花果多糖对糖尿病诱导大鼠血脂代谢指标的影响.【结果】无花果多糖提取最佳优化条件为酶解温度50.36℃,酶用量0.06 g和pH 4.73.该条件下无花果多糖提取预测值为34.47%,验证值为34.13%.用无花果多糖连续给大鼠灌胃给药28 d,发现无花果多糖能显著降低正常大鼠血糖(P<0.05),极显著降低肾上腺素引起的高血糖(P<0.01),有效降低糖尿病大鼠的 FBG、HbAlc、TC、TG,升高 HDL含量.【结论】

  14. 莲子红皮多糖提取工艺研究%Study on extraction technology of polysaccharides from red skin of locus seed

    Institute of Scientific and Technical Information of China (English)

    徐虹; 朱雨薇; 曹杨; 宋焕禄

    2011-01-01

    Red skin seeds of lotus usually produce about 15% of the red skin powder in the resurfacing process. It was determined that the polysaccharide content in red skin of lotus seed was 9.90%.For further developing the use value of lotus,the present study was designed to extract polysaccharides from the red skin powder using waterextraction and ethanol-precipitation method. With the polysaccharide yield as the index,the extraction conditions were optimized by the orthogonal-array-design on the basis of single-factor experiments. The results showed that the optimal conditions for extracting polysaccharides with α-amylase were as following :the particle size 100 mesh,the material/liquid ratio 1:15, and the enzymolysis time 105min. Under the above conditions, the polysaccharide yield was 8.42% ,and the purity of polysaccharide was 83.19%. It indicated that the red skin of lotus seeds had good development prospects.%红皮莲子在磨皮加工中会产生约15%的红皮粉.经测定,红皮粉中总多糖的含量为9.90%.为进一步开发和利用莲子的营养价值,本研究采用水提醇析法制备莲子红皮多糖.在单因素实验分析基础上,以多糖得率为指标,设计正交实验对提取条件进行了优化.结果表明,使用α一淀粉酶从莲子红皮中提取多糖,当料液比为1:15,酶解时间为105min,粉碎粒度为100目,多糖得率可达8.42%,多糖纯度为83.19%.由此可见,莲子红皮粉具有可开发利用的良好前景.

  15. A Microwave-Assisted Simultaneous Distillation and Extraction Method for the Separation of Polysaccharides and Essential Oil from the Leaves of Taxus chinensis Var. mairei

    OpenAIRE

    Chunjian Zhao; Xin He; Chunying Li; Lei Yang; Yujie Fu; Kaiting Wang; Yukun Zhang; Yujiao Ni

    2016-01-01

    An efficient microwave-assisted simultaneous distillation and extraction (MA-SDE) method has been developed for the separation of polysaccharides and essential oil from Taxus chinensis var. mairei. The key operating parameters for MA-SDE were optimized by single factor and central composite design experiments, and the optimal conditions were found to include a particle size of 60–80 mesh, liquid/solid ratio of 22.5 mL/g, extraction time of 17.5 min, microwave power of 547 W, and dichlorometha...

  16. 红豆杉多糖的提取与结构初探%Extraction of Maire Yew Polysaccharide and Its Structure

    Institute of Scientific and Technical Information of China (English)

    郑德勇; 徐莉; 郑月梅; 林清霞

    2012-01-01

    A polysaccharide was prepared from the branches and leaves of the Taxus ckinensis var. mairei by the processing of hot water extraction, concentration, alcohol-precipitation, dialysis and protein isolation successively. The polysaccharide molecular weight was determined by Sephadex G-100 gel permeation chromatography. The monosaccharide composition of the taxus polysaccharide was determined by gas chromatography. And IR spectra of taxus polysaccharide were determined, too. The results showed that the taxus polysaccharide' s molecular weight was 59. 2 ku, which composed of 365 monosaccharide groups. The ratio of Rhamnose, Arabinose, Mannose, Glucose and Galactose was 4:6:1:1:4. The taxus polysaccharide was a glycoconjugate that composed of several kinds of monosaccharide, glucuronic acid and amino acid. The monosaccharide was in the form of pyran ring and linked by β-( 1,3) glucosidic bond each other. The peptide in taxus polysaccharide couldn't be destructed by Sevag method.%红豆杉枝叶经热水浸提、浓缩、醇沉、透析等工序制得一种红豆杉多糖,采用Sephadex G-100凝胶渗透法测定分子质量,采用气相色谱法测定其单糖组成,并测定了不同纯度红豆杉多糖的红外光谱.所得红豆杉多糖分子质量约为59.2ku,可视为由约365个糖残基构成的高分子化合物,其单糖残基鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖数量比约为4∶6∶1∶1∶4.该红豆杉多糖分子结构中含有普通单糖、糖醛酸或氨基酸残基等,单糖主要以吡喃环形式存在、通过β-(1,3)苷键结合形成一种糖蛋白缀合物,Sevag法处理不能完全除去分子中的肽链.

  17. Extraction and Composition Analysis of Polysaccharides in Bulbus lilii%百合多糖提取及成分分析

    Institute of Scientific and Technical Information of China (English)

    张国强; 杨正平

    2012-01-01

    采用水提醇沉法提取百合(Bulbus lilii)中的多糖成分,Sevage法除蛋白质,凝胶色谱法进行多糖纯化,制备3-甲基-1-苯基-2-吡唑啉酮(PMP)衍生物进行高效液相色谱分析.结果表明,百合多糖由鼠李糖、阿拉伯糖、葡萄糖、木糖、半乳糖5种单糖组成,其含量分别为4.82%、12.79%、47.02%、13.01%、22.36%.%Polysaccharide was extracted and isolated from Bulbus lilii by water extracting-alcohol precipitating method and purified by Sevage method and gel permeation chromatography. The components of the polysaccharide were identified and quantified by HPLC of the PMP derivatives. The results showed that the main monosaccharides contained in B. Lilii polysaccharide were rhamnose, arabinose, glucose, xylose and galactose, with content of 4.82%, 12.79%, 47.02%, 13.01% and 22.36%, respectively.

  18. Polysaccharides-Rich Extract of Ganoderma lucidum (M.A. Curtis:Fr. P. Karst Accelerates Wound Healing in Streptozotocin-Induced Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Poh-Guat Cheng

    2013-01-01

    Full Text Available Ganoderma lucidum (M.A. Curtis:Fr. P. Karst is a popular medicinal mushroom. Scientific reports had shown that the wound healing effects of G. lucidum were partly attributed to its rich polysaccharides. However, little attention has been paid to its potential effects on wounds associated with diabetes mellitus. In this study, we evaluated the wound healing activity of the hot aqueous extract of G. lucidum in streptozotocin-induced diabetic rats. The extract of G. lucidum was standardised based on chemical contents (w/w of total polysaccharides (25.1%, ganoderic acid A (0.45%, and adenosine (0.069%. Six groups of six rats were experimentally wounded in the posterior neck region. Intrasite gel was used as a positive control and aqueous cream as the placebo. Topical application with 10% (w/w of mushroom extract-incorporated aqueous cream was more effective than that with Intrasite gel in terms of wound closure. The antioxidant activity in serum of rats treated with aqueous extract of G. lucidum was significantly higher; whereas the oxidative protein products and lipid damage were lower when compared to those of the controls. These findings strongly support the beneficial effects of standardised aqueous extract of G. lucidum in accelerating wound healing in streptozotocin-induced diabetic rats.

  19. The Study of Two Different Extraction Methods of Tamarind Seed Polysaccharide%两种罗望子多糖提取方法的研究

    Institute of Scientific and Technical Information of China (English)

    陈爱芬; 洪雪婷; 吴云英

    2015-01-01

    Two polysaccharide extraction methods of tamarind seed were analyzed and compared in the experiments.The result shows that the extraction rate of water extraction and ethanol precipitation is much higher than that of organic acid sedimentation,with the average extraction rate at 70.5% and 63.5%,respectively.However,in the term of the IR spectral result and some properties of tamarind seed polysaccharide,water extraction and ethanol precipitation is inferior to organic acid sedimentation.%采用水提醇沉法提和有机酸沉降法进行罗望子多糖的提取,并对两种方法进行了对比分析.结果显示:水提醇沉法提取罗望子多糖的平均提取率为70.34%,有机酸沉降法平均提取率为63.52%.从平均提取率看,水提醇沉法明显高于有机酸沉降法.但从红外光谱及部分性质的测定结果看,水提醇沉法提取纯度不及有机酸沉降法.

  20. Study on Extraction Technology for Polysaccharides in Pitaya Flower%热水法提取火龙果花多糖的工艺优化

    Institute of Scientific and Technical Information of China (English)

    高慧颖; 王琦; 陈源; 郑恒光; 余亚白

    2013-01-01

    采用热水法提取火龙果花多糖,研究不同的加热温度、料水比、加热时间等3个单因素对火龙果花多糖提取量的影响,采用L9(33)正交试验确定最佳提取工艺参数.结果表明:在加热温度90℃、料水比1∶35、加热时间3h的条件下,火龙果花多糖的提取效果最好,为55.87 mg·g-1.%Hot water was used for extracting polysaccharide from pitaya flower.Heating temperature,the ratio of solid to liquid,and heating time were three factors used separately in single factor test to analyze the influence of various factor on extraction efficiency.Orthogonal experiment design L9 (33) was applied to optimize the extraction parameters.The results showed that,with ratio solid ∶ liquid in 1 ∶ 35 and extraction temperature at 90℃ for 3h,the best extraction efficiency of polysaccharides from pitaya flower was obtained for 55.87 mg · g-1.

  1. Structural elucidation and immunostimulating property of a novel polysaccharide extracted from an edible mushroom Lentinus fusipes.

    Science.gov (United States)

    Manna, Dilip K; Maity, Prasenjit; Nandi, Ashis K; Pattanayak, Manabendra; Panda, Bibhash C; Mandal, Amit K; Tripathy, Satyajit; Acharya, Krishnendu; Sahoo, Atish K; Gupta, Nibha; Roy, Somnath; Islam, Syed S

    2017-02-10

    A water soluble heteroglycan (PS-II) with an average molecular weight∼60kDa was isolated from the hot aqueous extract of an edible mushroom Lentinus fusipes. The structural characterization of PS-II was carried out using total acid hydrolysis, methylation analyses, periodate oxidation, Smith degradation and 1D/2D NMR experiments. Total acid hydrolysis indicated the presence of D-galactose and D-glucose in a molar ratio of approximately 1:1. The chemical and NMR analyses revealed that the proposed repeating unit of the PS-II had a backbone chain consisting of three (1→6)-linked α-d-galactopyranosyl residue and two (1→6)-linked β-d-glucopyranosyl residues, one of the β-d-glucopyranosyl residue was branched at O-3 position with a terminal β-d-glucopyranosyl. The PS-II exhibited significant in vitro splenocyte and macrophage activations with optimum dose of 20μg/ml and 80μg/ml respectively. Flow cytometry study revealed the protective role of the PS-II against nicotine stimulated lymphocytes. Moreover, the ROS scavenging property of PS-II was also established using DPPH radical scavenging assay.

  2. In vitro proliferation and production of cytokine and IgG by human PBMCs stimulated with polysaccharide extract from plants endemic to Gabon.

    Science.gov (United States)

    Mengome, Line Edwige; Voxeur, Aline; Akue, Jean Paul; Lerouge, Patrice

    2014-11-13

    Polysaccharides were extracted from seven plants endemic to Gabon to study their potential immunological activities. Peripheral blood mononuclear cell (PBMC) (5×10⁵ cells/mL) proliferation, cytokine and immunoglobulin G (IgG) assays were performed after stimulation with different concentrations of polysaccharide fractions compared with lipopolysaccharides (LPS) and concanavalin A (ConA) from healthy volunteers. The culture supernatants were used for cytokine and IgG detection by enzyme-linked immunosorbent assay (ELISA). The results show that pectin and hemicellulose extracts from Uvaria klainei, Petersianthus macrocarpus, Trichoscypha addonii, Aphanocalyx microphyllus, Librevillea klaineana, Neochevalierodendron stephanii and Scorodophloeus zenkeri induced production levels that were variable from one individual to another for IL-12 (3-40 pg/mL), IL-10 (6-443 pg/mL), IL-6 (7-370 pg/mL), GM-CSF (3-170 pg/mL) and IFN-γ (5-80 pg/mL). Only hemicelluloses from Aphanocalyx microphyllus produce a small amount of IgG (OD=0.034), while the proliferation of cells stimulated with these polysaccharides increased up to 318% above the proliferation of unstimulated cells. However, this proliferation of PBMCs was abolished when the pectin of some of these plants was treated with endopolygalacturonase (p<0.05), but the trend of cytokine synthesis remained the same, both before and after enzymatic treatment or saponification. This study suggests that these polysaccharides stimulate cells in a structure-dependent manner. The rhamnogalacturonan-I (RGI) fragment alone was not able to induce the proliferation of PBMC.

  3. In Vitro Proliferation and Production of Cytokine and IgG by Human PBMCs Stimulated with Polysaccharide Extract from Plants Endemic to Gabon

    Directory of Open Access Journals (Sweden)

    Line Edwige Mengome

    2014-11-01

    Full Text Available Polysaccharides were extracted from seven plants endemic to Gabon to study their potential immunological activities. Peripheral blood mononuclear cell (PBMC (5 × 105 cells/mL proliferation, cytokine and immunoglobulin G (IgG assays were performed after stimulation with different concentrations of polysaccharide fractions compared with lipopolysaccharides (LPS and concanavalin A (ConA from healthy volunteers. The culture supernatants were used for cytokine and IgG detection by enzyme-linked immunosorbent assay (ELISA. The results show that pectin and hemicellulose extracts from Uvaria klainei, Petersianthus macrocarpus, Trichoscypha addonii, Aphanocalyx microphyllus, Librevillea klaineana, Neochevalierodendron stephanii and Scorodophloeus zenkeri induced production levels that were variable from one individual to another for IL-12 (3–40 pg/mL, IL-10 (6–443 pg/mL, IL-6 (7–370 pg/mL, GM-CSF (3–170 pg/mL and IFN-γ (5–80 pg/mL. Only hemicelluloses from Aphanocalyx microphyllus produce a small amount of IgG (OD = 0.034, while the proliferation of cells stimulated with these polysaccharides increased up to 318% above the proliferation of unstimulated cells. However, this proliferation of PBMCs was abolished when the pectin of some of these plants was treated with endopolygalacturonase (p < 0.05, but the trend of cytokine synthesis remained the same, both before and after enzymatic treatment or saponification. This study suggests that these polysaccharides stimulate cells in a structure-dependent manner. The rhamnogalacturonan-I (RGI fragment alone was not able to induce the proliferation of PBMC.

  4. Purification, characterization and antioxidant properties of a novel polysaccharide extracted from Sorghum bicolor (L.) seeds in sausage.

    Science.gov (United States)

    Ben Slima, Sirine; Ktari, Naourez; Trabelsi, Imen; Moussa, Hafedh; Makni, Iskandar; Ben Salah, Riadh

    2017-08-03

    A novel polysaccharide named Sorghum Water-Soluble Polysaccharide (SWSP) was purified from Sorghum bicolor (L.) seeds. It was structurally characterized by high performance liquid chromatography (HPLC), thin layer chromatography (TLC), Fourier transform-infrared spectroscopy analysis (FT-IR), X-ray diffraction (XRD) and mass spectroscopy (MS). HPLC and TLC showed that SWSP is a glucose polymer. The FT-IR spectrum proved the polysaccharide characteristic band of SWSP. XRD and microscopy analyses revealed that SWSP is a semi-crystalline polymer. Functional properties of SWSP were determined based on Water Holding Capacity (WHC), Oil Holding Capacity (OHC) and emulsification properties. SWSP showed good WHC and OHC, recorded at 3.01±0.03 and 1.02±0.03g/g, respectively and exhibited excellent emulsion properties even after 168h (61.5±0.02%). The effect of SWSP on oxidative stability of sausage during storage up to 12days at 4°C was investigated. Results showed a high rate (P<0.05) of oxymyoglobin and low lipid oxidation. The antioxidant activities of SWSP were also studied in vitro. Results demonstrated that the polysaccharides exhibited interesting 1,1-diphenyl-2-picrylhydrazyl (DPPH), ABTS radical scavenging, and ß-carotene bleaching inhibition activities. Overall, this natural polysaccharide was proved to enhance the oxidation stability of sausages, since it can efficiently substitute synthetic antioxidants in meat industry. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. 蜈蚣藻多糖的提取及其特性研究%Extraction and properties of polysaccharides from Grateloupia filicna

    Institute of Scientific and Technical Information of China (English)

    杨东娟; 杨永利; 王忠合; 郭守军; 刘伟明; 钟伟浩; 饶佩云

    2011-01-01

    分别采用碱处理水提法和KCl分级提取蜈蚣藻多糖,研究不同提取方法对蜈蚣藻多糖中3,6-内醚半乳糖、D-半乳糖、硫酸根及其总糖含量的影响。结果表明:粗多糖KCl分级为不溶胶多糖和可溶胶多糖,不溶胶多糖占0.68%,可溶胶多糖占86.25%;不溶胶多糖中硫酸根含量、总糖含量、3,6-内醚半乳糖含量和D-半乳糖含量分别为17.94%、21.27%、29.97%和20.65%,可溶胶多糖中硫酸根含量、总糖含量、3,6-内醚半乳糖含量和D-半乳糖含量分别为29.24%、53.76%、53.70%和32.78%。碱处理后多糖中硫酸根含量降低,3,6-内醚半乳糖含量增加,总糖含量无显著变化。体外细胞毒性测试结果表明,蜈蚣藻多糖对癌细胞株ECA-109具有较强的抑制作用。%The polysaccharides were extracted from Grateloupia filicina with hot water extraction, alkali extraction and the KCI classification extraction, and the properties were also studied, including the contents of the sulfate radical, the total sugar, 3,6-anhydro galactose, and D-galactose in the samples. The results indicated that the Grateloupia filicina polysaccharides could be fractionated to two kinds of polysaccharides, the KCl-insoluble section and the KCl-soluble section. The contents of the KCl-insoluble polysaccharides and the KCl-soluble polysaccharides were 0.68% and 86.25%. The content of the sulfate radical, the total sugar, 3, 6- anhydro galactose and the D-galactose in the KCl-insoluble polysaccharide were 17.94%, 21.27%, 29.97% and 20.65%, and 29.24%, 53.76%, 53.70% and 32.78% in the KCl-soluble polysaccharide. After treated with sodium hydroxide, the contents of 3,6-anhydro galactose and the D-galactose were incereased, but the contents of sulfate radical was reduced, and the contents of the total sugar were not changed.

  6. Comparing the sugar profiles and primary structures of alkali-extracted water-soluble polysaccharides in cell wall between the yeast and mycelial phases from Tremella fuciformis.

    Science.gov (United States)

    Zhu, Hanyu; Yuan, Yuan; Liu, Juan; Zheng, Liesheng; Chen, Liguo; Ma, Aimin

    2016-05-01

    To gain insights into dimorphism, cell wall polysaccharides from Tremella fuciformis strains were obtained from alkali-extracted water-soluble fractions PTF-M38 (from the mycelial form), PTF-Y3 and PTF-Y8 (from the yeast form) of T. fuciformis strains were used to gain some insights into dimorphism study. Their chemical properties and structural features were investigated using gel permeation chromatography, gas chromatography, UV and IR spectrophotometry and Congo red binding reactions. The results indicated that the backbones of PTF-M38, PTF-Y3 and PTF-Y8 were configured with α-linkages with average molecular weights of 1.24, 1.08, and 1.19 kDa, respectively. PTF-M38 was mainly composed of xylose, mannose, glucose, and galactose in a ratio of 1:1.47:0.48:0.34, while PTF-Y3 and PTF-Y8 were mainly composed of xylose, mannose and glucose in a ratio of 1:1.65:4.06 and 1:1.21:0.44, respectively. The sugar profiles of PTF-M38, PTF-Y3 and PTF-Y8 were also established for further comparison. These profiles showed that all three polysaccharides contained the same sugars but in different ratios, and the carbon sources (xylose, mannose, glucose, and galactose) affected the sugar ratios within the polysaccharides.

  7. 枸杞多糖的提取及其抗衰老的研究%Research on the Extraction and Anti-aging of Lycium Barbarum Polysaccharide

    Institute of Scientific and Technical Information of China (English)

    刘杰; 潘晓秋; 周晖; 张海林; 齐宏亮; 廖丹; 倪雪松

    2016-01-01

    目的:优选枸杞多糖的最佳提取工艺,通过建立D-半乳糖致衰老小鼠模型,研究枸杞多糖的抗衰老作用。方法:采用正交设计法对提取工艺进行研究;应用D-半乳糖连续注射,造成实验性衰老小鼠模型,通过测定血液丙二醛(MDA)、谷胱甘肽过氧化物酶( GSH-PX)和过氧化氢酶( CAT)的含量及超氧化物歧化酶( SOD)的活力和皮肤中SOD、MDA及羟脯氨酸( Hyp)的水平来检测枸杞多糖抗衰老的能力。结果:通过正交设计确定的枸杞多糖的最佳提取方案为:料液比为1∶30,提取2次,1次90 min,功率为100 kHz。通过研究可见,枸杞多糖可提高小鼠血液中SOD、CAT、GSH-px水平,降低MDA值;可提高小鼠皮肤SOD活力,降低皮肤MDA含量,提高Hyp含量。结论:本研究建立的提取方法简单、易行,枸杞多糖的提出率高。枸杞多糖具有抗D-半乳糖所致小鼠衰老的作用。%OBJECTIVE:To optimize the optimum extraction technology of lycium barbarum polysaccharide and establish senile mice model by D-galactose , so as to research the anti-aging effects of lycium barbarum polysaccharide . METHODS:Orthogonal design method was adopted to research the extraction technology .The senile mice model was established by injection of D-galactose .The anti-aging capacibility of lycium barbarum polysaccharide were determined by the detection of malonaldehyde (MDA), glutathione peroxidase (GSH-PX), the content of catalase (CAT), activity of superoxide dismutase (SOD) and level of SOD, MDA and hydroxyproline (Hyp) in the skin.RESULTS:The optimum extraction technology scheme by orthogonal design method were : the ratio of solid to liquid was 1∶30, 2 times of extraction, 90 min for once, the power was 100 kHz.Lycium barbarum polysaccharide could increase the level of SOD , CAT and GSH-px in rats , decrease MDA , improve the SOD activity , decrease MDA and increase the content of Hyp

  8. Proteolysis, NaOH and Ultrasound-Enhanced Extraction of Anticoagulant and Antioxidant Sulfated Polysaccharides from the Edible Seaweed, Gracilaria birdiae

    Directory of Open Access Journals (Sweden)

    Gabriel Pereira Fidelis

    2014-11-01

    Full Text Available The sulfated polysaccharides (SP from the edible red seaweed, Gracilaria birdiae, were obtained using five different extraction conditions: Gracilaria birdiae 1 (GB1-water; GB1s-water/sonication; GB1sp-water/sonication/proteolysis; GB2s-NaOH/sonication; and GB2sp-NaOH/sonication/proteolysis. The yield (g increased in the following order: GB2sp > GB1sp > GB2s > GB1s > GB1. However, the amount of SP extracted increased in a different way: GB2sp > GB1 > GB1sp > GB1s > GB2s. Infrared and electrophoresis analysis showed that all conditions extracted the same SP. In addition, monosaccharide composition showed that ultrasound promotes the extraction of polysaccharides other than SP. In the prothrombin time (PT test, which evaluates the extrinsic coagulation pathway, none of the samples showed anticoagulant activity. While in the activated partial thromboplastin time (aPTT test, which evaluates the intrinsic coagulation pathway, all samples showed anticoagulant activity, except GB2s. The aPTT activity decreased in the order of GB1sp > GB2sp > GB1 > GB1s > GB2s. The total capacity antioxidant (TCA of the SP was also affected by extraction condition, since GB2s and GB1 showed lower activity in comparison to the other conditions. In conclusion, the conditions of SP extraction influence their biological activities and chemical composition. The data revealed that NaOH/sonication/proteolysis was the best condition to extract anticoagulant and antioxidant SPs from Gracilaria birdiae.

  9. Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae.

    Science.gov (United States)

    Fidelis, Gabriel Pereira; Camara, Rafael Barros Gomes; Queiroz, Moacir Fernandes; Santos Pereira Costa, Mariana Santana; Santos, Pablo Castro; Rocha, Hugo Alexandre Oliveira; Costa, Leandro Silva

    2014-11-13

    The sulfated polysaccharides (SP) from the edible red seaweed, Gracilaria birdiae, were obtained using five different extraction conditions: Gracilaria birdiae 1 (GB1)-water; GB1s-water/sonication; GB1sp-water/sonication/proteolysis; GB2s-NaOH/sonication; and GB2sp-NaOH/sonication/proteolysis. The yield (g) increased in the following order: GB2sp>GB1sp>GB2s>GB1s>GB1. However, the amount of SP extracted increased in a different way: GB2sp>GB1>GB1sp>GB1s>GB2s. Infrared and electrophoresis analysis showed that all conditions extracted the same SP. In addition, monosaccharide composition showed that ultrasound promotes the extraction of polysaccharides other than SP. In the prothrombin time (PT) test, which evaluates the extrinsic coagulation pathway, none of the samples showed anticoagulant activity. While in the activated partial thromboplastin time (aPTT) test, which evaluates the intrinsic coagulation pathway, all samples showed anticoagulant activity, except GB2s. The aPTT activity decreased in the order of GB1sp>GB2sp>GB1>GB1s>GB2s. The total capacity antioxidant (TCA) of the SP was also affected by extraction condition, since GB2s and GB1 showed lower activity in comparison to the other conditions. In conclusion, the conditions of SP extraction influence their biological activities and chemical composition. The data revealed that NaOH/sonication/proteolysis was the best condition to extract anticoagulant and antioxidant SPs from Gracilaria birdiae.

  10. Optimization of the extraction technology of truffles polysaccharides by response surface analysis%响应面法优化块菌多糖的提取工艺

    Institute of Scientific and Technical Information of China (English)

    岳金玫; 蒲彪; 陈安均; 刘兴艳

    2012-01-01

    目的:优化热水浸提块菌多糖的提取工艺。方法:在单因素实验的基础上,采用3因素3水平的Box-Behnken中心组合设计确定热水提取条件。结果:在实验范围内各因素对块菌多糖得率和多糖含量的影响程度从大到小依次是提取温度〉液料比〉提取时间;最优工艺参数是提取温度90℃、提取时间60min、液料比(mLg)33:1,理论提取率为13.45%,理论含量为70.75%;实际提取率为13.32%,相对误差为0.96%;实际含量71.55%,相对误差为1.13%。结论:响应面法优化块菌多糖的提取工艺,简单可靠、效率高。%Purpose:To optimize the extraction conditions for polysaccharides from truffles.Methods:Based on the single-factor experiments,the Box-Behnken center-united experimental design for 3 factors were used to determine the extraction condition.Results:Within the tested ranges,the impact of three process parameters on extraction yield of polysaccharides from big to small was extraction temperature,liquid-to-solid ratio and extraction time.The optimum extraction temperature was 90℃,extraction time was 60min and liquid-to-solid ratio was 33:1 for polysaccharides extraction.The predictive maximum yield of polysaccharides extraction rates was 13.45% and the polysaccharide content was 70.75%,the average yield of polysaccharides in three validation experiment under the optimum process conditions was 13.32% and the relative error was 0.96%,the actual content was 71.55% and the relative error was 1.13%.Conclusion:The response surface optimized condition for extraction polysaccharides improved extraction efficiency,suggested which is suitable for the extraction of polysaccharides from truffles because of its reliability and briefness.

  11. Study on Composite Enzymatic Extraction of D.rubrovalvata Polysaccharides%复合酶法提取红托竹荪多糖的工艺研究

    Institute of Scientific and Technical Information of China (English)

    叶敏

    2012-01-01

    Composite enzymatic(cellulase,protease and pectase) extraction of D.rubrovalvata polysaccharides was studied in this paper.The main factors that affect extraction yield in the final product were analyzed,such as enzyma reaction temperature,pH,time and enzyma concentration.By means of single factor experiment and orthogonal design,optimum parameters were confirmed as enzyma reaction temperature 50℃,enzyma reaction pH4.5,enzyma reaction time 120 min and enzyma concentration 1.5%.The extraction yield of D.rubrovalvata polysaccharide is 7.98% under the above conditions.%采用复合酶法提取红托竹荪中的水溶性多糖,通过单因素试验和正交试验研究酶浓度、酶解时间、酶解温度以及酶解pH值对多糖得率的影响.确定复合酶法提取红托竹荪水溶性多糖的最佳条件为:酶浓度为1.5%,酶解时间120 min,酶解温度50℃,酶解pH值为4.5,此条件下的红托竹荪多糖得率为7.98%.

  12. Protective effect of Phellinus linteus polysaccharide extracts against thioacetamide-induced liver fibrosis in rats: a proteomics analysis

    Directory of Open Access Journals (Sweden)

    Wang Hualin

    2012-10-01

    Full Text Available Abstract Background The hepatoprotective potential of Phellinus linteus polysaccharide (PLP extracts has been described. However, the molecular mechanism of PLP for the inhibition of liver fibrosis is unclear. This study aims to investigate the molecular protein signatures involved in the hepatoprotective mechanisms of PLP via a proteomics approach using a thioacetamide (TAA-induced liver fibrosis rat model. Methods Male Sprague–Dawley rats were divided into three groups of six as follows: Normal group; TAA group, in which rats received TAA only; and PLP group, in which rats received PLP and TAA. Liver fibrosis was induced in the rats by repeated intraperitoneal injections of TAA at a dose of 200 mg/kg body weight twice a week for 4 weeks. PLP was given orally at a dose of 50 mg/kg body weight twice a day from the beginning of the TAA treatment until the end of the experiment. The development of liver cirrhosis was verified by histological examination. Liver proteomes were established by two-dimensional gel electrophoresis. Proteins with significantly altered expression levels were identified by matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry and the differentially expressed proteins were validated by immunohistochemical staining and reverse transcription polymerase chain reaction. Results Histological staining showed a remarkable reduction in liver fibrosis in the rats with PLP treatment. A total of 13 differentially expressed proteins including actin, tubulin alpha-1C chain, preprohaptoglobin, hemopexin, galectin-5, glutathione S-transferase alpha-4 (GSTA4, branched chain keto acid dehydrogenase hterotetrameric E1 subunit alpha (BCKDHA, glutathione S-transferase mu (GSTmu; glyceraldehyde-3-phosphate dehydrogenase (GAPDH; thiosulfate sulfurtransferase (TFT; betaine-homocysteine S-methyltransferase 1 (BHMT1; quinoid dihydropteridine reductase (QDPR; ribonuclease UK114 were observed between the TAA

  13. Effects of different extraction methods on yield of Smilax glabra polysaccharides and antioxidant activity%不同提取法对土茯苓多糖得率及抗氧化活性的影响

    Institute of Scientific and Technical Information of China (English)

    谢婷; 赵靖雯; 王敏; 易剑峰

    2015-01-01

    目的:考察不同提取方法对土茯苓多糖得率及抗氧化活性的影响。方法采用冷水、热水、氢氧化钠溶液、乙二胺四乙酸二钠(EDTA•2Na)水溶液提取土茯苓多糖,测定清除羟自由基活性、对超氧阴离子自由基的清除作用、还原能力,评价土茯苓多糖的体外抗氧化能力。结果冷水溶粗多糖、热水溶粗多糖、碱溶粗多糖、酸溶粗多糖的多糖得率分别为0.31%、1.1%、10.8%、2.0%。4种方法提取所得土茯苓粗多糖清除超氧自由基的能力均较好;冷水溶粗多糖、热水溶粗多糖、酸溶粗多糖清除羟自由基的能力及还原能力较好,且随浓度增加而上升;碱溶粗多糖基本无清除羟自由基的能力及还原能力。结论4种方法提取所得粗多糖均具有一定的抗氧化活性,且各组分的体外抗氧化活性与多糖含量呈正相关性,结合得率,EDTA•2Na 水溶液提取法最好。%Objective To explore the effects of different extraction methods on yield of Smilax glabra polysaccharides and antioxidant activities. Methods Crude polysaccharides were extracted by using cold water, hot water, NaOH aqueous solution, and EDTA•2Na aqueous solution . Antioxidant activities of Smilax glabra polysaccharides were compared by the methods of scavenging superoxide anion radical , scavenging hydroxyl radical or reducing power. Results Yield of crude cold water-soluble polysaccharides, crude boiling water-soluble polysaccharides, crude alkali-soluble polysaccharides and crude acid-soluble polysaccharides were 0.31%, 1.1%, 10.8 %, 2.0%, respectively. Polysaccharides by using four kinds of different extraction methods had strong scavenging superoxide anion radical power; crude cold water-soluble polysaccharides, crude boiling water-soluble polysaccharides , crude acid-soluble polysaccharides had strong scavenging hydroxyl radical and reducing power , the capacity increased with the increasing

  14. 蝙蝠蛾被孢霉多糖的分离纯化及理化性质%Extraction and characterization of polysaccharides from Mortierella hepiali

    Institute of Scientific and Technical Information of China (English)

    刘佩佩; 缪小亮; 尹鸿萍; 奚涛

    2012-01-01

    Purpose To study the isolation and purification of polysaccharide from Mortierella hepialid. Methods The raw polysaccharide(CPSM) was extracted with water and the content of total of polysaccharide was determined with anthrone-sulfuric acid. Protein was determined by Bradford. Monosaccha-ride was detected by thin-layer chromatography. Two fractions of polysaccharide were obtained by Cellulose DE-52 column chromatography, then were purified respectively by Sephadex column chromatography to obtain CPSM-1a and CPSM-2. Physicochemical Properties of the two polysaccharides were studied by HPLC, FTIR, UV, GPC analysis. Results The content of total polysaccharide, and protein in CPSM was 61.4% and 1.8% respectively,and monosaccharide was not detected. CPSM-la was composed of Man, Gal,Glc with a molar ratio of 5:2:3. CPSM-2 was composed of Man,GalUA,Gal,Glc and Xyl with a molar ratio of 5:1:2:1:1. The weight-average molecular weight of CPSM-la and CPSM-2 were estimated to be 8.8×103,7.6×103 respectively. Conclusion CPSM-la is a neutral polysaccharide,and CPSM-2 is an acidic polysaccharide.%目的 提取蝙蝠蛾被孢霉多糖,并对其理化性质进行研究.方法 利用水提醇沉法得到粗多糖CPSM,采用硫酸-蒽酮法测定粗多糖含量,考马斯亮蓝法测定蛋白质含量,薄层色谱法检查是否含有单糖.CPSM经纤维素DE-52柱分离得CPSM-1和盐洗脱多糖.两种多糖分别经Sephadex系列凝胶柱分离纯化得到CPSM-1a和CPSM-2,采用光谱法和色谱法测定其单糖组成、分子质量等.结果 粗多糖CPSM糖含量为61.4%,蛋白含量为1.8%,不含还原糖.CPSM-1a主要含有甘露糖、半乳糖和葡萄糖,其摩尔比约为5:2:3;CPSM-2主要含有甘露糖、半乳糖醛酸、半乳糖、葡萄糖和木糖,其摩尔比约为5:1:2:1:1.CPSM-1a和CPSM-2重均分子质量分别为8.8×103和7.6×103.结论 CPSM-1a为一水溶性中性多糖,CPSM-2为一水溶性酸性多糖.

  15. 响应面分析法优化牛蒡根多糖提取工艺%Optimization of Extraction Technology for Polysaccharide from Roots of Arctium lappa by Response Surface Methodology

    Institute of Scientific and Technical Information of China (English)

    李瑾; 侯淑珍; 王景媛; 王娜

    2012-01-01

    目的:优化牛蒡根多糖的提取工艺.方法:采用水提醇沉法提取多糖,以多糖得率为考察指标;采用响应面分析法研究影响牛蒡根多糖测定的因素,以多糖提取率为响应值作响应面和等高线.结果:牛蒡多糖提取工艺的最佳条件为料液比1∶14.43,浸提温度84.85℃,浸提时间3.81 h.此条件下,牛蒡多糖的提取率为6.16%.结论:该优选工艺稳定、可行.%Objective; To optimize extraction technology of polysaccharide from roots of Arctium lappa. Method; Polysaccharide was extracted by water extraction and alcohol precipitation method with yield of polysaccharide as index; Response surface methodology was used to study on influencing factors of determination of polysaccharide from roots of A. lappa, response surface and contour were made with extract ratio of polysaccharide as response value. Result; Optimum extraction technology conditions were solid-liquid ratio of 1= 14. 43, extraction temperature 84. 85 t , extracting lime 3. 81 h. Under these conditions, yield of polysaccharide from roots of A. lappa was 6. 16%. Conclusion; This optimized technology was stable and feasible.

  16. An exocellular polysaccharide and its interactions with proteins

    NARCIS (Netherlands)

    Tuinier, R.

    1999-01-01

    In the food industry polysaccharides are used as thickening or gelling agents. Polysaccharides are usually extracted from plants. Micro-organisms are also capable of excreting polysaccharides: exocellular polysaccharides (EPSs). In some cases EPSs are produced in-situ in food products, notably in ac

  17. Antioxidant and antiedema properties of solid-state cultured honey mushroom, Armillaria mellea (higher Basidiomycetes), extracts and their polysaccharide and polyphenol contents.

    Science.gov (United States)

    Lai, Min-Nan; Ng, Lean Teik

    2013-01-01

    Culinary-medicinal honey mushroom or Mi-Huan-Ku, Armillaria mellea (AM), is a popular ingredient in the traditional Chinese medicine for treating diseases of geriatric patients. This study aimed to examine the effect of cultured substrates on the mycelial growth of AM and evaluate its antioxidant and antiedema activities as well as its total polysaccharide and polyphenol contents. Results showed that AM grew best on the maize medium and worst on the potato medium. AM ethanol extract (AM-EtOH) showed stronger DPPH radical scavenging activity than AM aqueous extract (AM-H₂O). However, they were weak in metal chelation and reducing power. AM-EtOH but not AM-H₂O at 200 mg/kg showed antiedema activity in rats. The total β-glucan content of AM-H₂O and AM-EtOH was 21.95% and 3.50%, respectively. AM-EtOH showed higher phenol but lower flavonoid content than AM-H₂O. These results indicate that maize is a good source of substrate for mass production of AM mycelia, and its potency of DPPH radical scavenging and antiedema activities was contributed mainly by the phenolic compounds, not the level of polysaccharide content.

  18. 超声波辅助提取双孢菇多糖的研究%Study on the polysaccharides extraction of Agaricus bisporus using ultrasonic

    Institute of Scientific and Technical Information of China (English)

    高振鹏; 袁亚宏; 岳田利; 杨昊博

    2012-01-01

    【Objective】 Study was made on the optimum technology of Agaricus bisporus polysaccharides extraction by using ultrasonic to lay the foundation for solving by-products reuse in the canning process.【Method】 Using Agaricus bisporus canning by-products as raw materials,single factor experiment and orthogonal test were adopted to analyze the effect of each factor in solid-liquid ratio,ultrasonic power,extraction time and temperature on the extraction percentage of the polysaccharides using ultrasonic,and the process parameters were optimized.【Result】 The results indicated that the optimum condition of extraction is as follows:liquid-solid ratio 40 mL/g,ultrasonic power 560 W,time 120 min and temperature 60 ℃.【Conclusion】 Under this optimum condition,the extraction percentage of Agaricus bisporus polysaccharides reached 3.65%,which increased by 96.24% compared with the common technical yields.%【目的】对超声波辅助提取双孢菇多糖的工艺进行研究,为双孢菇罐头加工过程中废弃物的综合利用提供支持。【方法】以盐渍双孢菇罐头废弃物为原料,通过单因素及正交试验探讨了液料比、超声波功率、超声波处理时间及处理温度对双孢菇多糖提取率的影响,并对超声波辅助提取双孢菇多糖的工艺参数进行了优化。【结果】优化得到超声波辅助提取双孢菇多糖的最佳工艺参数为:超声波功率560W,超声波处理时间120min,超声波处理温度60℃,液料比40mL/g。【结论】在优化的最佳工艺条件下,双孢菇多糖的提取率为3.65%,较无超声波促进作用下普通工艺的多糖提取率提高了96.24%。

  19. Cellulase Extraction Technology of Polysaccharide from Dendrobium Nobile%纤维素酶提取金钗石斛多糖工艺研究

    Institute of Scientific and Technical Information of China (English)

    张晓敏; 孙志蓉; 朱南南; 程丽丽; 杨春宁; 王晨

    2015-01-01

    ive:Objective:To optimize the parameters of the cellulose extraction of Polysaccharide from Dendrobi-um nobile .Mothods:Regard the extraction rate of polysaccharide as indexes,and use orthogonal experriments to determine the content of index in cellulose by UV Spectrophotometry.Then evaluate the merits of enzymatic parameters.Results:The optimized process conditions were solid-to-liquid ratio 1:50,pH 5.0,reflux time 170min,dosage of enzyme 4500U/g,enzymolysis temperature 55℃,and the Dendrobium nobile polysaccharide extraction rate was 7.71%,with 43.84% higher than conventional thermal reflow method.Conclusion:This method has advantages of simplicity,high extraction rate,which lays a foundation for the further study of Den-drobium nobile.It also has the potentially industrial application value in the extraction of effective ingredients of Chinese Medicine.%目的:对纤维素酶用于金钗石斛多糖提取工艺的酶解参数进行优选。方法:通过正交实验,以多糖提取率为指标,采用紫外分光光度法测定多糖含量,以此评价酶解工艺的优劣。结果:最佳酶解条件为:料液比1∶50,pH值5.0,提取时间170min,酶用量4500U/g,酶解温度55℃,此时金钗石斛多糖提取率为7.71%,比常规热回流提取方法高43.84%。结论:本方法装置简单,提取率高,为金钗石斛深入研究奠定基础,在中药有效成分的提取方面具有潜在的工业应用价值。

  20. Optimum Technique Research on the Extraction of Bergamot Crude Polysaccharide with Ethanol Subsiding Method%水提—醇沉法提取佛手粗多糖的最佳工艺研究

    Institute of Scientific and Technical Information of China (English)

    纪耀华; 袁湘杰

    2011-01-01

    目的:确定利用传统方法提取佛手粗多糖的最佳工艺条件。方法:以佛手多糖的含量为指标,以提取时间、提取次数、提取温度、料液比为提取因素,通过单因素试验确定因素水平,利用统计分析方法对提取佛手粗多糖的工艺进行优化研究。结论:水提—醇沉法提取佛手粗多糖的最佳工艺条件是:提取温度95℃,浸提时间2h,料液比1:15,提取次数2次,此方法可以为工业大规模生产佛手多糖提供依据。%【Objective】To determine the optimum condition of total sugar content of crude polysaccharide from bergamot.【Method】 Using polysaccharide content of bergamot as indicators,the extraction time,extraction times,extraction temperature,solid to liquid ratio as extraction factors,factor level were determined through single factor experiment,and the extraction optimization of bergamot crude polysaccharide was researched by statistical method.【Conclusion】 The optimum conditions of ethanol subsiding method to extract total sugar content of crude polysaccharides from bergamot were extraction temperature 95℃,extraction time 2h,solid to liquid ratio 1:15,and extraction twice.This method will provide basis for the mass industrial production of bergamot polysaccharide.

  1. 画眉草多糖提取及其保湿性能研究%Extraction and Moisture Retention of Polysaccharides from Eragrostis Teff

    Institute of Scientific and Technical Information of China (English)

    祝士惠; 孙培冬; 李海洋

    2013-01-01

    本文以脱脂画眉草为原料,用纤维素酶超声辅助法提取多糖;经脱蛋白、脱色、DEAE-纤维素分离纯化,得到3种多糖TPS1、TPS2、TPS3,提取率分别为0.42%、0.66%、0.54%.通过高效凝胶渗透色谱(HPGPC)分析,3种多糖的分子量分别为7886 Da、8467 Da、6366 Da;高效离子色谱(HPIC)测得TPS1组成单糖为果糖,TPS2、TPS3组成单糖为阿拉伯糖、半乳糖、葡萄糖、木糖、甘露糖、果糖、核糖和一种未知糖.对多糖TPS2的保湿和吸湿性研究结果表明TPS2的保湿和吸湿能力均优于常用保湿剂甘油、聚乙二醇400、1,3-丁二醇、壳聚糖.%The polysaccharides in the defatted eragrostis teff were extracted with cellulose enzyme and ultrasound auxiliary. The TPS1 ,TPS2 and TPS3 which were the main polysaccharides parts of eragrostis teff were obtained through purification on DEAE-cellulose, after the polysaccharides were taken off the protein with enzyme and decolored with activated carbon. Extraction ratios of the TPS1 ,TPS2 and TPS3 were 0.42% ,0.66% and 0.54%. The molecular weight of TPS1, TPS2 and TPS3 were 7886 Da,8467 Da and 6366 Da respectively measured by high-performance gel permeation chroma-tography. The components of the polysaccharides were detected by ion chromatography. The results showed that TPS1 contains fructose. Both TPS2 and TPS3 was composed of Ara,Glu,Gla,Xyl,Man,Fru,Rib and an unknown sugar. Meanwhile the moisture retention and absorption performance of the TPS2 were studied. The results showed that the TPS2 moisture absorption and retention capacity are better than glycerol, polyethylene glyeol 400 and chitosan.

  2. Effects of Different Extraction Methods on Antioxidant Activity of Inonotus obliquus Polysaccharides%不同提取方法对桦褐孔菌多糖抗氧化活性的影响

    Institute of Scientific and Technical Information of China (English)

    张丽霞; 张伟娜; 李凌智; 张虹

    2012-01-01

    [ Objective ] This study aimed to explore the effects of different extraction methods on antioxidant activities of /. Obliquus polysaccharides. [ Method ] Crude polysaccharides were extracted by using boiling ethanol precipitation method and ethanol-alkali extraction method; water-soluble and alkali-soluble polysaccharides were purified by using trichloroacetic acid method; antioxidant activities of /. Obliquus polysaccharides were compared by hydroxyl radical scavenging experiment and superoxide anion radical scavenging experiment. [ Result] Yield of crude water-soluble polysaccharides, crude alkali-soluble polysaccharides, pure water-soluble polysaccharides and pure alkali-soluble polysaccharides were 14.0% , 27.7% , 7.0% and 6.2% , respectively. Hydroxyl radical scavenging effect of crude water-soluble polysaccharides and pure alkali-soluble polysaccharides had achieved the maximum with polysaccharide concentration of 0.9 mg/ml and 0.06 mg/ml, and the scavenging rates were 54. 3% and 10.9% , respectively; superoxide anion radical scavenging effect of crude water-soluble polysaccharides and pure water-soluble polysaccharides had achieved the maximum with polysaccharide concentration of 0. 1 mg/ml and 0. 07 mg/ml, and the scavenging rates were 24. 2% and 27. 2% , respectively. [ Conclusion] Antioxidant activity of water-soluble polysaccharides was higher than that of alkali-soluble polysaccharides. /. Obliquus could be used as a species of new efficient natural antioxidant medicinal fungi in application.%[目的]研究不同提取方法对桦褐孔菌多糖抗氧化活性的影响.[方法]采用水煮醇沉法和碱液醇沉法提取粗多糖;采用三氯乙酸法纯化水溶和碱溶粗多糖;采用羟基自由基清除试验和超氧阴离子自由基清除试验进行抗氧化活性对比研究.[结果]水溶粗多糖、碱溶粗多糖、水溶纯多糖、碱溶纯多糖的多糖得率分别为14.0%、27.7%、7.O%、6.2%.对羟基自由

  3. Extraction, Purification and Antioxidantion of Polysaccharide from Purple Sweet Potato%紫红薯多糖的提取纯化及抗氧化作用研究

    Institute of Scientific and Technical Information of China (English)

    李翔; 上官新晨; 蒋艳; 吴少福; 陈继光

    2011-01-01

    The technology for extraction of polysaccharide from purple sweet potato was optimized by orthogonal array design. After removal of pigment and protein by X - 5 and sevag method respectively, Ⅰ and Ⅱ of polysaccharide portions were obtained by further purification by cellulose Chromatography DEAE. Addition ally , the antioxidant capacity of the purified polysaccharide was evaluated by determining their DPPH ? And ? OH and ABTS scavenging activities. The results indicated that the obtained optimum conditions are material to wa ter ratio 1:8, extraction time 60 min, extraction power 240 W. The yield of polysaccharide is 5.44% under the optimal conditions. After purification;Ⅰ and Ⅱ of polysaccharide portions showed considerable antioxidant capacity.%实验优化了从紫红薯中提取多糖的工艺,利用DEAE - 52柱层析分离纯化出紫红薯多糖Ⅰ和Ⅱ两个组分,并测定了对·OH、DPPH·和ABTS的清除作用.结果表明:紫红薯多糖最佳提取条件为提取时间60 min、料液比为1:8、超声功率为240W,经纯化后的紫红薯多糖I和Ⅱ具有较好的清除自由基的能力.

  4. Optimization of ultrasonic-assisted extraction of polysaccharide from lotus seed skins%超声波辅助提取莲子皮多糖工艺优化

    Institute of Scientific and Technical Information of China (English)

    李杨; 周坚; 万盈; 陈轩

    2011-01-01

    Ultrasonic-assisted extraction of polysaccharide from lotus seed skins was studied. The single factor test and the orthogonal test were used to study the effect of liquid/material ratio, extraction time, ultrasonic power and extraction times on extraction rate of polysaccharide from lotus seed skins. The results showed that the optimal process conditions for ultrasonicassisted extraction of polysaccharide from lotus seed skins were as follows: liquid/material ratio: 30:1, extraction time:15min, ultrasonic power: 60 W and extraction times: 4; under such conditions, the extraction rate of polysaccharide was 17.37%, and the mass fraction of polysaccharide was 63.75%.%以莲子皮为原料,研究其多糖的超声波辅助提取工艺条件.采用单因素试验和正交试验,探讨液料比、提取时间、超声功率、提取次数对莲子皮多糖提取率的影响.并以多糖提取率为评价指标,优化提取工艺.试验结果表明:超声波辅助提取莲子皮多糖的最佳工艺条件为液料比30:1,提取时间为30 min,超声功率为60 W,提取次数为4次.在此条件下,多糖提取率为17.37%,多糖质量分数为63.75%.

  5. 硫酸酯化修饰葛仙米多糖工艺研究%Sulfation Modification of Polysaccharide Extracted from Nostoc sphaeroides Ktzing

    Institute of Scientific and Technical Information of China (English)

    朱玉婷; 谭姚; 莫开菊

    2011-01-01

    The orthogonal array design method was used to optimize three reaction conditions,including esterification reagent,temperature and reaction time,for the sulfation of crude polysaccharides extracted from Nostoc sphaeroides Ku..tzing by water extraction and subsequent alcohol precipitation.Besides,FTIR spectroscopic analysis was carried out to identify the structural difference of Nostoc sphaeroides Ku..tzing polysaccharides as a result of the sulfation reaction,and a correlation analysis was done between FTIR A1261/A1418 and degree of substitution(DS) of sulfated polysaccharides,as determined by the barium chloride-gelation method.The optimal sulfation reaction conditions were found to be: 1:4 chlorosulfonic acid-pyridine as esterification reagent for 6 h reaction at 70 ℃.Under the optimal sulfation conditions,the DS of the final products was 1.042.Meanwhile,the sulfated polysaccharide obtained revealed typical sulfated functional groups.The correlation coefficient between FTIR A1261/A1418 and DS of sulfated Nostoc sphaeroides Ku..tzing polysaccharides was 0.974.Therefore,infrared spectroscopy can be used to characterize the structural difference of sulfated polysaccharides and quantify the DS of sulfate groups.%采用氯磺酸-吡啶法合成硫酸酯化葛仙米多糖,利用正交设计对酯化试剂比例、反应温度及反应时间进行优化。通过傅里叶红外光谱分析酯化前后的结构差异,氯化钡-明胶比浊法测定取代度,并分析红外光谱法与取代度之间的相关性。结果表明:葛仙米多糖硫酸酯化修饰的最佳条件为V(氯磺酸)与V(吡啶)比例1:4、反应温度70℃、反应时间6h,此条件下取代度达到1.042;红外光谱分析表明,硫酸酯化后的葛仙米多糖具有硫酸酯键的特征吸收峰,其吸光度比值A1261/A1418与化学方法所测得的硫酸酯化取代度的相关系数达到0.974。红外光谱不仅可以表征硫酸酯化多

  6. Black Fungus Polysaccharides Extraction and Determination%黑木耳多糖的微波提取及含量的测定

    Institute of Scientific and Technical Information of China (English)

    李超; 王磊; 任遥; 朱东; 唐志华; 叶凌枫

    2012-01-01

    Using microwave assisted extraction of wild black fungus polysaccharides to improve the traditional extraction process,the single factor and orthogonal experiment to determine the optimum microwave extraction conditions.The results showed that the experiments for determining the reasonable extraction conditions were: using water as extraction solvent,the microwave power was 560 W,the microwave extraction time was 40 s,the solid to liquid ratio of 1∶130,the extraction time 4 h.Obtained by the single factor experiment all factors in the order of extraction timemicrowave extraction timepowerratio of water.%采用微波法辅助提取野生黑木耳多糖,改进了传统的浸提工艺,通过单因素和正交试验确定微波辅助提取的最佳工艺条件。结果表明,合理提取工艺条件为:采用水作为提取剂、微波功率为560 W、微波提取时间为40 s、料液比为1∶130、萃取时问为4 h浸提效果最佳。由单因素试验得出各因素的影响大小顺序为浸提时间〉微波提取时间〉功率〉料水比。

  7. 香菇多糖提取工艺的比较研究%Study on Extraction Technology of Polysaccharides in Shiitake Mushroom

    Institute of Scientific and Technical Information of China (English)

    张素霞

    2009-01-01

    The effects of supercritical CO2 extraction technology and traditional hot-water method on the shiitake mush-room polysaecharide extraction was compared. The results showed that the optimum conditions of supercritical CO2 fluid extraction were: pressure of 10 Mpa, temperature of 40℃, time of I h. After supereritical CO2 extracting degreasing, the time of the hot water immersion proposes was 1 h, only 1/5 time to the conventional hot-water immersion, the extraction rate of polysaccharide was 6.87%. The extraction rate is 3.16% higher than the traditional water bath soak. The ultraviolet absorption spectrometry of the product showed that there was few impurity. The infrared spectrum indicated that the struc-ture is consistent with the direct hot water immersion formulation. After using the supercritical CO2 extraction, the extrac-tion rate and the quality of shiitake mushroom polysaccharide was obviously enhanced.%比较研究了超临界CO2辅助提取法和传统热水提取法2种工艺对香菇多糖提取效果的影响.试验结果表明,超临界CO2萃取的最佳工艺为:萃取压力10 Mpa,萃取温度40%,萃取时间1 h.超临界CO2萃取脱脂后,热水浸提时间为1 h.仅用了传统方法热水浸提时间的1/5,香菇多糖提取率为6.87%,比传统热水浸提法提高3.16%,所得产品紫外吸收光谱显示,产品杂质含量少,红外光谱显示结构与直接热水浸提法一致.采用超临界CO2辅助提取后,香菇多糖的提取率和质量显著提高.

  8. Study on extraction and purification of Ginkgo biloba exocarp polysaccharide%银杏外种皮多糖的提取和纯化工艺研究

    Institute of Scientific and Technical Information of China (English)

    陈静静; 江波; 张涛; 沐万孟; 缪铭

    2011-01-01

    考察温度、料液比、时间以及提取次数对多糖提取率的影响,确定最佳提取条件.水提醇沉得到的银杏外种皮中多糖(GBEP)含有一定的蛋白质,以多糖的损失率和蛋白质的去除率为指标,比较三种方法:三氯乙酸法、Sevage法、酶法去除GBEP中蛋白质的效率.结果表明,用酶法除蛋白时,多糖的损失率最低;Sevage法除蛋白最彻底,重复多次操作后可以得到不含蛋白质的多糖,虽然多糖损失较多,但有利于进一步的实验研究.AB-8树脂对多糖脱色率为66.35%.%Ginkgo biloba exocarp polysaccharide(GBEP) was obtained from exocarp of Ginkgo biloba L by hot water extraction and ethanol precipitation.The crude GBEP contains a significant amount of protein,which should be eliminated before further investigation.Three deproteinization methods: trichloroacetic acid treatment, Sevage method,enzyme treatment were evaluated,with protein removal rate and polysaccharide loss rate as efficiency criteria.Enzymatic method exhibited the minimum polysaccharide loss,while protein can be completely removed by Sevage method. The decolorization efficiency was 66.35% when using AB-8 macroporous adsorption resin.

  9. Study on Optimum Extracting Conditions of Total Polysaccharides from Ganoderma lucidum of Lingdan Capsule by Alkaline Solutions%碱水提取复方灵丹胶囊中灵芝多糖的工艺

    Institute of Scientific and Technical Information of China (English)

    叶豆丹; 孟美; 王化宇; 任钰; 张炜煜

    2014-01-01

    试验优选了碱水提取法提取复方灵丹胶囊中灵芝多糖的最佳工艺。以总多糖的含量为考察指标,采用单因素法和正交试验法确定方中灵芝多糖最佳提取工艺。结果最佳提取工艺为加10倍于药材量的水(pH=9)煎煮2次,每次1.0 h。该提取工艺操作简单、稳定可行,适合于产业化生产,并具有良好的应用前景。%To optimize the extraction technology of effective fraction of Ganoderma lucidum. To regard the contents of total polysaccharides as the examinational indicators, to decide the extraction methods by L9 (34) orthogonal test and single factor method for total polysaccharides components of Ganoderma lucidum. The optimal extraction of total polysaccharides in Ganoderma lucidum is medicinal materials plus 10 times the amount of water (pH=9), extract twice and 1.0 h per time. The optimum extraction technology of total polysaccharides from Ganoderma lucidum is simple, stable and feasible, suitable for industrial production, and has a good prospect.

  10. Extraction,Purification and Properties Researches of Hemi-cellulose Polysaccharides from Pineapple Peel%菠萝皮渣半纤维素多糖提取纯化及性质研究

    Institute of Scientific and Technical Information of China (English)

    吴靖; 黄惠华

    2009-01-01

    The pineapple hemi-cellulose polysaccharides,obtained from pineapple peel by NaOH solution extraction and ethanol precipitation,were separated and then purified by DEAE-Cellulose( OH-) column. The optimal processing of pineapple hemi-cellulose polysaccharides extraction was:extracting time of 2h,ratio of material and 7% NaOH ( w: v) 1: 10,extracting temperature of 40℃. The yield of hemi-cellulose and content of pentosans are 41. 3% and 73 % respectively. Three kinds of purified hemi-cellulose polysaccharides by freeze-drying were obtained. The hemi-cellulose polysaccharides-1,2 were basically not composed of monosaccharide,starch,protein and nucleic acid. The hemi-cellulose polysaccharides-2 was showed a typical polysaccharide FT-IR spectrum profile.%通过正交试验优化了碱提醇沉菠萝皮渣半纤维素粗多糖提取条件,并用DEAE-Cellulose(OH-)纯化得到3种半纤维素多糖组分,初步分析了半纤维素多糖的理化性质.结果表明:半纤维素粗多糖最佳提取条件为时间2 h,NaOH质量分数7%,固液比(g∶mL)1∶10,温度40℃;菠萝粗半纤维素得率和聚戊糖含量分别为41.3%和73%;柱层析菠萝半纤维素多糖-1和2基本不含单糖、淀粉、蛋白质、核酸类物质;红外分析表明菠萝半纤维素多糖-2具有典型多糖吸收图谱.

  11. 南瓜籽多糖热水提取工艺优化及其抗氧化活性%Extraction technology optimization and antioxidant activity of polysaccharides from pumpkin seeds

    Institute of Scientific and Technical Information of China (English)

    王丽波; 程龙; 徐雅琴; 王泰恒; 李鲁滨; 王瀚嵩

    2016-01-01

    Pumpkin seeds are rich in lipids, proteins, phytosterols, vitamins, minerals and other valuable components. In recent years, many researches have showed pumpkin seeds can significantly reduce serum cholesterol and triglycerides, and play a role in the prevention and mitigation of cardiovascular disease as well as in preventing prostate disease. It has been found that natural polysaccharides extracted from plant seeds possess various important biological activities, such as anti-cancer, antioxidant, immunization and hypolipidemic activities. However, there is not much published information on polysaccharides in pumpkin seeds. For the development and utilization of polysaccharides in pumpkin seeds, at present, degreased pumpkin seed powder was used as raw material, and the extraction of polysaccharides was mainly performed by hot-water extraction. The optimum extraction technology was studied and the preliminary structure of polysaccharides was analyzed by using infrared spectrum scanning (IR) and gas chromatography (GC). Taking polysaccharide yield as an index, the effects of extraction time, extraction temperature, liquid-solid ratio were studied through the single-factor experiment. And then on the basis of single-factor experiment, the optimum extraction condition was investigated with the response surface methodology (RSM) according to the Box-Behnken center combination design principle by the Design-Expert 8.0 software. The results showed that the optimum parameters were: extraction time 2.5 h, extraction temperature 60 °C, liquid-solid ratio 40:1 mL/g, and the extraction yield of pumpkin seed polysaccharides was 2.25%. Under this condition, the actual value was 2.18%, which was closely matched with the predicted yield. The result showed that, the developed model was adequate and precise when compared with experimental data. The monosaccharide composition of polysaccharide was analyzed through comparing the retention time against the standards by GC

  12. Extraction optimization by response surface methodology of mucilage polysaccharide from the peel of Opuntia dillenii haw. fruits and their physicochemical properties.

    Science.gov (United States)

    Han, Yu-Lu; Gao, Jie; Yin, Yan-Yan; Jin, Zheng-Yu; Xu, Xue-Ming; Chen, Han-Qing

    2016-10-20

    In this study, response surface methodology (RSM) was employed to optimize microwave-assisted extraction (MAE) technology of mucilage polysaccharide from the peel of Opuntia dillenii haw. fruits (OFPP), and the physicochemical characteristics of OFPP were also investigated. The three parameters were the ratio of water to raw material (30-40ml/g), microwave power (300-400W) and extraction time (120-180s). The results indicated that the yield of OFPP was 15.62±0.37% under the optimum extraction conditions. Compared with MAE, the OFPP yield by hot water extraction (HWE) was 13.36±0.71%. In addition, the rheological properties of OFPP were also explored. The OFPP obtained by HWE exhibited a lower viscosity compared with that by MAE. The FT-IR spectra analysis, scanning electron microscopy (SEM) observation and thermogravimetric analysis (TGA) revealed that there were strong interactions between Ca(2+) and OFPP, which resulted in the high viscosity, different microstructure and thermal stability of OFPP.

  13. Study on microwave-assisted extraction of garlic polysaccharide and its antioxidant activity%微波辅助大蒜多糖的提取及其抗氧化活性的研究

    Institute of Scientific and Technical Information of China (English)

    邵金华; 李涛; 杨佳

    2013-01-01

    研究了微波辅助大蒜多糖的提取工艺及抗氧化活性.结果表明:最佳功率为300 W,提取温度为65℃,提取时间为8 min,料液比1∶7(g·mL-1);大蒜多糖有一定的清除DPPH和OH·自由基的作用,与Vc相比,大蒜多糖对DPPH和OH·自由基的清除效果要差;但大蒜多糖对菜籽油的抗氧化能力较Vc强,能够有效抑制菜籽油的氧化.%The microwave-assisted extraction of polysaccharide from garlic was studied in this paper.Moreover,we also investigated the antioxidant feature of the extracted garlic polysaccharide.The optimum extraction conditions were that 300 W of microwave power,8 min of extraction time and the rate between material and solvent was 1∶7 (g·mL-1).Although the garlic polysaccharide showed certain scavenging effect on the DPPH and hydroxyl radical,but it was not as good as vitamin C.However,the garlic polysaccharide was more effective than vitamin C in protecting the camellia seed oil from being oxidized.

  14. 宁夏沙枣多糖的酶法提取工艺%Study on Enzymatic Extracting Conditions of Polysaccharide from Elaeagnus angustifolia L.in Ningxia

    Institute of Scientific and Technical Information of China (English)

    王凯; 王青

    2011-01-01

    研究酶法提取宁夏沙枣(Elaeagnus angustifolia L.)中多糖的提取工艺.在单因素试验的基础上,采用正交设计考察了不同pH值、酶的添加量、酸提时间对多糖提取率的影响.结果表明,最佳工艺条件为酶提pH值4.38,添加酶的质量分数5%,酶提时间3h,酶提温度60℃.最佳条件下多糖的提取率为72.93%.%The enzymatic extracting conditions of polysaccharide from Elaeagnus angustifolia L. in Ningxia was studied. Based on single factor test, the effects of pH, enzyme dosage, enzymolysis time on polysaccharide extracting rate were investigated and optimized by orthogonal test. The results showed that the optimum conditions for extracting polysaccharide from E. angustifolia by enzyme were pH 4.38, enzyme dosage 5%,enzymolysis time 3h, enzymolysis temperature 60℃. Under optimal conditions, the extracting rate of polysaccharide was 72.93%.

  15. Production Practice of Russian Standard-based Welding Process for K60 Linepipe%执行俄罗斯标准的K60管线管JCO焊接工艺实践

    Institute of Scientific and Technical Information of China (English)

    尚才众; 郑丽华; 贾云刚; 吴建国

    2015-01-01

    分析了俄罗斯标准K60管线管母材的焊接性,介绍了K60管线管焊材选择、焊接坡口设计及焊接方法以及生产检测结果.认为:选用碱度值较高的焊剂,注意调配焊缝金属中Mn、Si含量,以保证组织中含有较多的针状铁素体;焊接热输入对K60钢级管线管焊缝组织影响明显,随着焊接热输入的增加,柱状晶比例减小,针状铁素体呈先增后减的趋势.

  16. Extraction of rice bran polysaccharides by enzyme combined with ultrasound and its antioxidant activity%酶法协同超声波提取米糠多糖及其抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    魏明; 王晨; 杨超英; 钱森和

    2015-01-01

    The effect of enzyme combined with ultrasound treatments on the extraction of polysaccharides from rice bran was investigated. The extraction conditions of polysaccharides from rice bran were opti-mized by response surface methodology, and antioxidant activity of rice bran polysaccharides was researched. The results showed that both ultrasound and compound enzymes ( mass ratio of cellulase to neutral protease 1∶1) treatments could improve the extraction of rice bran polysaccharides. The optimal extraction conditions of rice bran polysaccharides were obtained as follows:dosage of compound enzymes 3. 1 mg/mL, enzymolysis time 2 h, ultrasonic power 198 W, ultrasonic time 20 min, ratio of material to liquid 1∶30, extraction time 3. 2 h, extraction temperature 60℃. Under these conditions, the yield of rice bran polysaccharides reached 5 . 3%. The rice bran polysaccharides had strong reducing power and certain antioxidant capability on oil and its scavenging effects on DPPH· and ·OH were significant.%研究了酶法协同超声波处理对米糠多糖提取的影响,利用响应面法对米糠多糖提取工艺进行了优化,并探讨了米糠多糖的抗氧化活性。结果表明,纤维素酶与中性蛋白酶复配使用(质量比1∶1)以及超声波处理有利于米糠多糖的提取。提取米糠多糖的最佳条件为:复合酶加量3.1 mg/mL,酶解时间2 h,超声功率198 W,超声时间20 min,料液比1∶30,提取时间3.2 h,提取温度60℃;在此条件下米糠多糖得率达到5.3%。米糠多糖具有较强的还原力和一定的抗油脂氧化活性,对DPPH自由基、羟基自由基均表现出较好的清除能力。

  17. Effect of ultrasonic extraction conditions on antioxidative and immunomodulatory activities of a Ganoderma lucidum polysaccharide originated from fermented soybean curd residue.

    Science.gov (United States)

    Shi, Min; Yang, Yingnan; Hu, Xuansheng; Zhang, Zhenya

    2014-07-15

    A crude Ganoderma lucidum polysaccharide (GLPL) was extracted from fermented soybean curd residue by ultrasonic assisted extraction. The optimal extraction conditions were 30 min at 80 °C with 80 W and water to solid ratio of 10, and with this method 115.47 ± 2.95 mg/g of GLPL yield was obtained. Additionally, the antioxidant and immunomodulatory activities of GLPL were investigated. The results showed that GLPL exhibited strong antioxidant effects, which included scavenging activities against DPPH radicals, hydrogen oxide and ABTS radicals with IC50 values of 0.23, 0.48 and 0.69 mg/mL, respectively. For immunomodulatory activities, GLPL was shown to strongly stimulate the proliferation of macrophages (158.02 ± 13.12%), the production of nitric oxide and phagocytosis (21.16 ± 1.65 μM), and, at 40.00 μg/mL, protected macrophage from Doxorubicin (DOX) (0.16 ± 0.003).

  18. Extraction, separation and structural characterization of polysaccharides from Neverita didyma%扁玉螺多糖的提取、分离和结构分析

    Institute of Scientific and Technical Information of China (English)

    邢晓旭; 赵峡; 李大敬; 于广利; 殷秀红

    2013-01-01

    目的 从扁玉螺(Neverita didyma)中提取和分离纯化多糖,并对其基本理化性质和结构组成进行分析.方法 依次采用水提和碱提方法从扁玉螺中提取粗多糖,采用DEAE Sepharose FF阴离子交换和Sephacryl S-300凝胶柱层析对粗多糖进行分离纯化,并对其总糖、蛋白、氨基糖、糖醛酸和硫酸根含量,相对分子质量和单糖组成进行分析.对多糖纯化组分采用甲基化、气质联用(GC-MS)、红外光谱(IR)、电喷雾质谱(ESI-MS)和核磁共振波谱(NMR)对其化学结构进行分析.结果 扁玉螺水提粗多糖(BYL-S)中不含有糖醛酸和硫酸基,其单糖组成只含Glc,进一步分离得到了4种水溶性多糖组分.碱提粗多糖(BYL-J)单糖组成相对复杂,除含有Glc外,还含有Man、GlcN、GalN、Gal和Fuc,其摩尔比为Glc∶Man∶GlcN∶GalN∶Gal ∶ Fuc=78.9∶ 1.7∶ 3.4∶ 2.2∶ 5.2∶5.6,进一步分离得到了3种多糖组分.对水提多糖纯化组分BYL-S2的结构分析表明其是以旷(1→4)为主链,含有少量β-(1→3,4)和β-(1→3)分支的D-吡喃型葡聚糖.结论 从扁玉螺中提取分离得到了7种多糖组分,并确定了一种水溶性葡聚糖的结构,为扁玉螺多糖结构和活性的深入研究提供了基础.%Objective To extract and purify polysaccharides from Neverita didyma,and investigate their chemical properties and structural features.Methods Neverita didyma polysaccharides were extracted by distilled water and alkali solution sequentially,and purified by DEAE Sepharose FF anion-exchange and Sepharcryl S-300 gel-permeation chromatography.The contents of total sugar,protein,uronic acid,sulfate and amino sugar were determined by various chemical methods,and monosaccharide compositions were analyzed by high performance liquid chromatography.The structure of polysaccharides was characterized by methylation analysis,GC/MS,FT-IR,ESI-MS and NMR techniques.Results No uronic acid,sulfate and amino sugar were found in the

  19. Extraction of Sulfated Polysaccharide from Kelp%海带中硫酸酯化多糖提取研究

    Institute of Scientific and Technical Information of China (English)

    张彦民; 李宝才; 朱利平

    2003-01-01

    硫酸酯化多糖(sulfated polysaccharides,SPS)具有多种生物活性,采用了单因素分析法对硫酸酯化多糖的提取工艺条件进行了优选研究.确定了最佳条件为:提取时间为2 h,提取为温度90℃,加水量为干净海藻质量的25倍.SPS产率达到1.99%,其主要理化指标"硫酸根+多糖"质量分数为61.26%.

  20. Study on the Extraction of Polysaccharide from Rhodiola Rosea and Its Antioxidant Activity in Vitro%红景天多糖的提取及体外抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    林晓月; 齐珈娆; 王丹丹; 任婷; 佟海滨; 孙新

    2016-01-01

    Objective To establish the optimum process for the extraction of Rhodiola rosea polysaccharides ( RSP ) ,and to evaluate their free radical scavenging capacities. Method The optimum extraction conditions were obtained by orthogonal experiment design. Taking the crude polysaccharide content as index,the influences of solid-liquid ratio,extraction temperature,extraction time,extraction times on the extraction efficiency were compared. The proteins in Rhodiola rosea polysaccharides were removed by repeated freezing and thawing,dialysis,and enzyme-sevag method,in order to acquire relatively pure polysaccharides. The basic physical and chemical properties of Rhodiola rosea polysaccharides were characterized by phenol-sulfuric acid method, infrared chromatography and UV-visible spectrophotometer. The antioxidant system was introduced in vitro. The scavenging effects of Rhodiola rosea polysaccharides on three free radicals were determined,including 1,1-diphenyl-2-picrylhydrazyl (DPPH ·), hydroxyl radical ( · OH ) and superoxide anion ( O-2 · ) . Results The optimal extraction conditions were as follows:solid-liquid ratio of 1 ∶ 20,extraction temperature of 90 ℃,3 extraction times,extraction time of 3 h. In addition,Rhodiola rosea polysaccharides exhibited excellent scavenging abilities on DPPH · and · OH free radicals. Conclusion Optimum extraction technology of Rhodiola rosea polysaccharide is successfully established, in addition,Rhodiola rosea polysaccharides have certain antioxidant effect.%目的:优化红景天多糖的制备工艺,并系统评价红景天多糖清除自由基的能力。方法采用正交设计方法优化红景天多糖提取工艺,以多糖提率为考察指标,比较不同固液比、提取温度、提取时间、提取次数对多糖提取效果的影响;通过反复冻融、透析及酶-sevag联合脱蛋白法,纯化制备红景天多糖( RSP );采用苯酚-硫酸法、红外色谱仪及紫外-可见分光光度计对红景天

  1. Optimization of microwave assisted the extraction of phellinus polysaccharides using response surface methodology%响应面法优化微波辅助提取桑黄子实体多糖的工艺研究

    Institute of Scientific and Technical Information of China (English)

    秦俊哲; 任金玫; 殷红

    2014-01-01

    Based on single-factor studies ,response surface optimization was carried out to opti-mize the extraction process of Phellinus polysaccharides by the microwave-assisted extrac-tion technology .The results showed that polysaccharide yield was most significantly affected by microwave power ,extraction time and solid/liquid ratio .The optimal conditions of extrac-tion as follows :extraction time 5 .1 min ,microwave power 540 W ,solid/liquid ratio 1∶41 (w/v) ,extraction twice .Under such conditions ,the yield of Phellinus linteus polysaccharide could reach 4 .18% .%本文以桑黄子实体为原料,在单因素实验的基础上,运用响应曲面法优化微波辅助提取桑黄多糖的工艺条件.结果表明:对桑黄多糖得率的影响因素按主次排序为:微波功率>液料比>提取时间.确定最佳工艺参数为:微波处理时间5.1 min、微波功率540 W、提取2次,在此工艺条件下,桑黄多糖得率为4.18%.

  2. 金佛手果叶多糖提取工艺的优化%Optimization of Extracting Conditions of Polysaccharides from the Fruit and Leaf of Citrus bergamot

    Institute of Scientific and Technical Information of China (English)

    杨继; 陆智明; 陆国权

    2011-01-01

    Through single factor and multi-factor experiments,and response surface experimental design method, the best extraction process of polysaccharides by water extraction of the Citrus bergamot is studied.The result shows that:for polysaccharides,the optimal extraction conditions is 247 minutes of extracting time,91℃of extracting temperature, and the ratio of sample to extracting reagent with 1:64.Polysaccharides yield in Citrus bergamot was 4.542 and 1.776 g/100 g,respectively.Reliable experiment verified the reliability of the above optimized condition.%通过单因素和多因素实验,采用响应面实验设计方法对金佛手果叶多糖以水提取的最佳提取工艺进行了研究,结果表明:多糖最佳提取组合是提取时间是247min,温度是91℃,料液比是1:64,此时1g金佛手果、叶粉多糖得率分别是4.542和1.776g/100g。可靠性验证实验表明,上述提取方法的优化条件是可靠的。

  3. The removal of polysaccharide in three plant DNA extraction methods%3种植物DNA提取法中多糖类物质去除效果的研究

    Institute of Scientific and Technical Information of China (English)

    高洪晓; 杨凯; 刘建斌

    2011-01-01

    To find an efficient method for the removal of polysaccharide in plant DNA extract process, the CTAB, high salt and Chlorobenzene methods were used to extract the genome DNA of three Syringa plants. Agarose gel electrophoresis and ultraviolet spectrophotometer methods were adopted to test the DNA quality, and the concentration changes of polysaccharid in the DNA extraction process were tested with anthraquinone hydrate-sulfuric. The result showed that the removal efficiency of polysaccharide in the three methods were similar. The high salt method was safer and convenienter than the other two methods. The influence of polysaccharide in the high salt methods was removed in a special step. The result provided theoretical basis for the removal of polysaccharide in plant DNA extraction process.%为探明DNA提取过程中去除多糖的有效方法,以丁香属3种植物为材料,用3种DNA提取方法(普通CTAB法、氯苯法和高盐法)提取丁香属植物基因组DNA.对提取的DNA质量采用琼脂糖凝胶电泳和紫外分光光度计法进行检测,并采用蒽酮硫酸法测定DNA提取过程中多糖含量的变化.结果表明:3种方法的多糖去除率相近且都比较高.高盐法与前两种方法相比对人体和环境危害较小且操作简单,是最适合该属植物的DNA提取方法.该方法采用了专门的去除多糖的步骤,为DNA提取过程中多糖的去除提供了一定的理论依据.

  4. Optimization of the Microwave Extraction Condition of Colored-Mushroom Polysaccharides using Response Surface Analysis%响应面法优化微波萃取花菇多糖

    Institute of Scientific and Technical Information of China (English)

    董丹丹; 何晋浙; 孙培龙

    2011-01-01

    This research adopted the microwave extraction method,which researched the effects of four factors on mushroom polysaccharides, such as extraction time, microwave power, microwave temperature and liquid to solid ratio. Based on the response surface analysis with the Design-Expert software program, the extraction condition of the microwave for colored-mushroom polysaccharides was optimized. The results indicated that compared to the hot water extraction and ultrasonic method, microwave increased 1.02% and 1.18% yield of colored-mushroom polysaccharides respectively, and when the microwave power was 700 W, the order of the remaining three parameters on the extraction yield of polysaccharides was extraction time>microwave temperature>liquid to solid ratio. When the microwave treatment was conducted at 88 ℃ for 21 min, the microwave power was 700 W and the liquid to solid ratio was 69, under these conditions, the mushroom polysaccharides yield reached 8.80%, and the polysaccharide yield of second extraction was 1.79%.%采用响应面法对花菇多糖进行微波萃取工艺研究.考察工艺参数——提取时间、微波功率、提取温度、液料比4个因素对花菇多糖得率的影响,用Design-Expert软件程序对实验数据进行二次响应面分析,同时做上述工艺参数的优化实验.实验结果表明:1)相较于热水浸提法与超声波法,微波提取法使多糖得率分别提高了1.02%和1.18%;当微波功率为700W时,其余3项工艺参数对花菇多糖提取得率的影响次序为提取时间>提取温度>液料比.2)优化的微波提取花菇多糖的工艺条件是:提取时间21 min,微波功率700W,提取温度88℃,液料比69.在该条件下花菇多糖得率8.80%,多糖二次萃取得率1.79%.

  5. Ultrasonic-assisted Water Extraction of Polysaccharides from Portulaca and Its Antioxidant Activity%金荞麦多糖的超声提取及抗氧化作用研究

    Institute of Scientific and Technical Information of China (English)

    谷仿丽; 黄仁术; 孙红玉; 刘玉曼

    2014-01-01

    以多糖得率为指标,对影响多糖提取率的超声时间、温度、料液比进行单因素和正交试验;以清除自由基和抗猪油氧化作用探讨金荞麦多糖的抗氧化能力。结果表明,影响金荞麦多糖提取率的最主要因素是时间,最佳提取工艺条件为超声时间120 min、温度60℃、料液比1:20;金荞麦多糖可清除自由基,降低猪油过氧化值,具有体外抗氧化作用。%The extraction rate was used as index to evaluate the results of ultrasonic of polysaccharide from Portulaca ,in which 3 factors considered were extraction time ,extraction temperature ,the solvent volume .The antioxidant capacity of polysaccharide from Portulaca was studied by eliminating free radicals and antioxidant effects on fats .The results showed that the main factors affecting the extraction rate of Portulaca polysaccharide was time ,the best extraction condition were as liquid:ultrasonic time 120min ,temperature 60 ℃ ,ratio of solid to liquid 1 :20 .The extraction of polysaccharide from Portulaca has good antioxidant activity in vitro by eliminating free radicals and reducing peroxide value of fats .

  6. Important Determinants for Fucoidan Bioactivity: A Critical Review of Structure-Function Relations and Extraction Methods for Fucose-Containing Sulfated Polysaccharides from Brown Seaweeds

    Directory of Open Access Journals (Sweden)

    Anne S. Meyer

    2011-10-01

    Full Text Available Seaweeds—or marine macroalgae—notably brown seaweeds in the class Phaeophyceae, contain fucoidan. Fucoidan designates a group of certain fucose-containing sulfated polysaccharides (FCSPs that have a backbone built of (1→3-linked α-l-fucopyranosyl or of alternating (1→3- and (1→4-linked α-l-fucopyranosyl residues, but also include sulfated galactofucans with backbones built of (1→6-β-d-galacto- and/or (1→2-β-d-mannopyranosyl units with fucose or fuco-oligosaccharide branching, and/or glucuronic acid, xylose or glucose substitutions. These FCSPs offer several potentially beneficial bioactive functions for humans. The bioactive properties may vary depending on the source of seaweed, the compositional and structural traits, the content (charge density, distribution, and bonding of the sulfate substitutions, and the purity of the FCSP product. The preservation of the structural integrity of the FCSP molecules essentially depends on the extraction methodology which has a crucial, but partly overlooked, significance for obtaining the relevant structural features required for specific biological activities and for elucidating structure-function relations. The aim of this review is to provide information on the most recent developments in the chemistry of fucoidan/FCSPs emphasizing the significance of different extraction techniques for the structural composition and biological activity with particular focus on sulfate groups.

  7. Important determinants for fucoidan bioactivity: a critical review of structure-function relations and extraction methods for fucose-containing sulfated polysaccharides from brown seaweeds.

    Science.gov (United States)

    Ale, Marcel Tutor; Mikkelsen, Jørn D; Meyer, Anne S

    2011-01-01

    Seaweeds--or marine macroalgae--notably brown seaweeds in the class Phaeophyceae, contain fucoidan. Fucoidan designates a group of certain fucose-containing sulfated polysaccharides (FCSPs) that have a backbone built of (1→3)-linked α-L-fucopyranosyl or of alternating (1→3)- and (1→4)-linked α-L-fucopyranosyl residues, but also include sulfated galactofucans with backbones built of (1→6)-β-D-galacto- and/or (1→2)-β-D-mannopyranosyl units with fucose or fuco-oligosaccharide branching, and/or glucuronic acid, xylose or glucose substitutions. These FCSPs offer several potentially beneficial bioactive functions for humans. The bioactive properties may vary depending on the source of seaweed, the compositional and structural traits, the content (charge density), distribution, and bonding of the sulfate substitutions, and the purity of the FCSP product. The preservation of the structural integrity of the FCSP molecules essentially depends on the extraction methodology which has a crucial, but partly overlooked, significance for obtaining the relevant structural features required for specific biological activities and for elucidating structure-function relations. The aim of this review is to provide information on the most recent developments in the chemistry of fucoidan/FCSPs emphasizing the significance of different extraction techniques for the structural composition and biological activity with particular focus on sulfate groups.

  8. Integrated method of thermosensitive triblock copolymer-salt aqueous two phase extraction and dialysis membrane separation for purification of lycium barbarum polysaccharide.

    Science.gov (United States)

    Wang, Yun; Hu, Xiaowei; Han, Juan; Ni, Liang; Tang, Xu; Hu, Yutao; Chen, Tong

    2016-03-01

    A polymer-salt aqueous two-phase system (ATPS) consisting of thermosensitive copolymer ethylene-oxide-b-propylene-oxide-b-ethylene-oxide (EOPOEO) and NaH2PO4 was employed in deproteinization for lycium barbarum polysaccharide (LBP). The effects of salt type and concentration, EOPOEO concentration, amount of crude LBP solution and temperature were studied. In the primary extraction process, LBP was preferentially partitioned to the bottom (salt-rich) phase with high recovery ratio of 96.3%, while 94.4% of impurity protein was removed to the top (EOPOEO-rich) phase. Moreover, the majority of pigments could be discarded to top phase. After phase-separation, the LBP in the bottom phase was further purified by dialysis membrane to remove salt and other small molecular impurities. The purity of LBP was enhanced to 64%. Additionally, the FT-IR spectrum was used to identify LBP. EOPOEO was recovered by a temperature-induced separation, and reused in a new ATPS. An ideal extraction and recycle result were achieved.

  9. 块菌多糖提取工艺优化及粗多糖抗氧化性的测定%Optimization of Extraction Technique of Polysaccharides from Truffles by Response Surface Methodology and Determination of Crude Polysaccharide Antioxidant Activity

    Institute of Scientific and Technical Information of China (English)

    孔庆龙; 樊建; 赵天瑞

    2012-01-01

    In order to set up the optimization of water extraction technique of polysaccharides from truffles, the yield of crude polysaccharides was chosen as the index, based on the principle of Box-Benhnken Center combination experimental design, the optimization of extraction technique of polysaccharides was carried out by three factors on three levels. The results showed the highest extraction ratio (10. 8% ) was obtained at 94. 68℃, extraction 3. 71 h, and Water-solid ratio of 58. 66. Evaluation of antioxidant activity is measured by DPPH radical scavenging activity, Hydroxyl radical scavenging activity and Reducing power. The results indicate that crude polysaccharide of truffles has a good antioxidant activity.%为确定块菌多糖水提适宜工艺条件,以块菌粗多糖得率为指标,根据Box-Benhnken的中心组合试验设计原理,在单因素试验的基础上采用三因素三水平的响应面法对提取工艺进行优化.结果表明:在温度为94.68℃,提取时间为3.71 h,液料比值58.66时提取率最高为10.8%.采用DPPH自由基清除法、羟基自由基清除法、还原力检测法对块菌粗多糖的抗氧化活性进行测定,结果表明块菌粗多糖具有较好的抗氧化活性.

  10. 微波辅助提取酸浆籽中多糖方法优化%Optimization of the Extraction of Polysaccharides from Physalis alpepengi Defatted Feed by Microwave-assisted

    Institute of Scientific and Technical Information of China (English)

    于开源; 鞠晓峰; 宫春颖

    2012-01-01

    采用微波辅助提取技术,研究微波功率、处理时间及料液比对脱脂酸浆籽中多糖提取率的影响。运用minitabl5.0数据分析软件,采用Box-Behnken的中心组合设计,采用三因素三水平的响应面法优化工艺条件,建立微波提取多糖的二次多项数学模型,并以多糖的提取率为响应值做响应面,得到优化条件为:料液比1:12,微波功率610w,微波处理时间83s。在此条件下实际多糖提取率为5.57%。%Using microwave assisted extraction, microwave power, time and solid to liquid ratio of polysaccharide extraction rate in the defatted seed oil. With minitablS.0 data analysis software, using the Box-Behnken, central composite design with three factors and three levels of response surface method to optimize the process conditions, microwave extraction of polysaccharide secondary to a] number of mathematical models, and rate of response to the polysaccharide extract. The value of doing response surface has been] optimized conditions as follows: liquid ratio 1:12, microwave power 610 W, microwave treatment time 83 s. Under this condition, the] actual polysaccharide extract was 5.57%.

  11. Optimization of the Extraction Technique and the Antioxidant Activity of Polysaccharides from Pleurotus geesteranus%秀珍菇子实体多糖的提取工艺优化及体外抗氧化性

    Institute of Scientific and Technical Information of China (English)

    杨润亚; 李维焕; 吕芳芳

    2012-01-01

    采用响应面法优化了秀珍菇子实体中水溶性多糖的超声提取工艺,并初步测定了多糖提取物的抗氧化活性.结果表明,在提取温度为40℃、超声功率为400W、料液比为1∶30、浸提时间114 min的最佳提取工艺条件下,响应面拟和所得方程对秀珍菇多糖的最大提取率预测值60.74mg/g,实测值60.19 mg/g,实测结果与预测值符合良好.秀珍菇的多糖提取物具有清除羟基自由基的作用,在一定范围内随着多糖浓度的增加其清除效果度还原力均加强.%The ultrasonic extraction process of polysaccharides from Pleurotus geesteranus was optimized by response surface analysis,and the antioxidant activity of the extracts were determined. The results showed that,the optimum ultrasonic extraction conditions were obtained as follows; temperature at 40 ^.ultrasonic power at 400 W,time duration for 114 min,and the ratio of solution (water) to solid (fruit body powder) of 30:1. Under this condition,the extraction yield of the polysaccharide from Pleurotus geesteranus was 60.19 mg/g,which was consistent with the predicted value (60.74 mg/g). The Polysaccharide extracts have a certain extent function to scavenging hydroxyl radical. Their antioxidant power and the reducing capacity increased with the increase of polysaccharides concentration.

  12. 微波协同酶法提取金针菇多糖工艺的优化%Preparation of polysaccharides from Flammulina velutipes by microwave-enzyme synergistic extraction

    Institute of Scientific and Technical Information of China (English)

    黄琼; 丁玲

    2013-01-01

    采用微波协同酶法提取金针菇多糖,研究提取条件对金针菇多糖得率的影响,并在单因素试验基础上进行正交试验.结果表明:微波协同酶法提取可以有效提高金针菇多糖得率,其最佳提取工艺条件为酶解温度45℃,酶解时间1.5h,调节溶液pH 4.0,加入0.40%(m∶m)果胶酶,微波功率600 W,料液比1∶20(m∶V),提取时间90 s,提取3次,在最佳条件下,金针菇多糖得率为21.76%.%Microwave-enzyme synergistic method was used to extract the polysaccharides from Flammulina velutipes , and the effects of extracting conditions on the yield of Flammulina velutipes polysaccharides was studied through the single factors and orthogonal test. Results showed that microwave-enzyme synergistic method could increase the yield of Flammulina velutipes polysaccharides effectively. The optimal extraction conditions were obtained as follows: enzy-molysis temperature 45℃, enzymolysis time 1.5 h, pH 4.0, concentration of pectinas 0.4% (m : m), microwave power 600 W, ratio of solid-liquid 1:20(m : V), extraction time 90 s, and times extraction 3. Under the optimal conditions, the yield of Flammulina velutipes polysaccharides by using microwave-enzymatic synergistic method was 21.76%.

  13. 嗜酸乳杆菌胞外多糖提取工艺优化%The Optimization of Extro-polysaccharides of Lactobacillus acidophilus in Extraction

    Institute of Scientific and Technical Information of China (English)

    刘齐; 刘爱红; 孙美玲; 李孚杰

    2011-01-01

    Lactobacillus acidophilus, which is recognized as a safe fermentation creature, is probiotics which can stay in human intestinal wall. Many strains have been the stars in the food industry for a long time. The extro-polysacchrides of bacteria are macromolecule polymers which are secreted during the incubation or in the cell wall to protect the bacteria during incubation. The polysacchrides are diluted in the water. Its perfect characteristics in rheology and physics has put it on the stage for research and production. The extro-polysacchrides of lactobacii are not only important for flavor and quality of dairy, but also a secure source of polysaccharides, because of its capability of stabilizing and emulsifying the humidity of diary product. In this article, extraction of the extro-polysacchrides of lactobacillus acidophilus is optimized with the SAS software. The yield of polysaccharides is 1.91 g/L by extraction 80% ethanol (1:1.8) for 12hours.%微生物的胞外多糖是微生物在生长代谢过程中分泌到细胞壁外的黏液多糖或荚膜多糖,是具有增稠和胶体性质并能溶解或分散在水中的长链、高分子聚合物.试验以嗜酸乳杆菌胞外多糖为研究对象,对提取时间、提取的乙醇体积分数和提取的料液比进行单因素实验,并利用sas软件对其提取工艺进行优化,得到在80%体积分数的乙醇下,以1:1.8(体积比)的料液比抽提12 h,得到的多糖的产量为1.91g/L.

  14. Extraction Process and Antioxidant Activity of Pinus taeda L.Polysaccharides%火炬松松针多糖提取工艺及其抗氧化性研究

    Institute of Scientific and Technical Information of China (English)

    许小向; 尹小莉; 洪艳平; 邹凯; 上官新晨; 梁强

    2016-01-01

    With the polysaccharides yield as target , the polysaccharides from pine needles of Pinus taeda L.was extracted and its antioxidant activity in vitro was determined .The ultrasonic-assisted extraction technology of polysaccharides from pine needles of P.taeda was confirmed by using Box-Behnken response surface methodology .The results showed that the optimal extraction process conditions were ultrasonic time 25 min, water extraction time 1 h, liquid-material ratio of 25∶1 ( mL∶g) and extraction temperature 91 ℃.Under optimal conditions , the yield of polysaccharides was up to 1.867%.The antioxidant activity in vitro was evaluated by measuring the DPPH· , · OH and ABTS + free radical removal ability.As a result, the pine needle polysaccharides had a relatively strong capacity to scavenge DPPH ,OH and ABTS +free radical .They had a better concentration-response relationship .It showed that pine needle polysaccharides had an obvious antioxidant activity .%以多糖得率为指标,从火炬松松针中浸提多糖,并测定其体外抗氧化活性。在单因素试验基础上采用响应面法优化超声波辅助热水浸提火炬松松针多糖,最佳的工艺参数为:30 g松针粉末在超声波作用时间25 min,热水浸提1 h,液料比25∶1(mL∶g),热水浸提温度91℃。在此条件下,火炬松松针多糖得率达1.867%,提取率达91.39%。通过测定松针多糖对苯基苦基肼自由基( DPPH·)、羟基自由基(· OH)和2,2-联氮-二-(3-乙基-苯并噻唑-6-磺酸)自由基(ABTS+)的清除能力评价其体外抗氧化能力。结果显示:火炬松松针多糖对自由基DPPH·、· OH和ABTS+都有较强清除能力,且都呈较好的量效关系,火炬松松针多糖具有较强的体外抗氧化能力。

  15. Optimization of Ultrasonic-assisted Extraction of Polysaccharides from Chinese Yam Peel and Its Antioxidant Activity%山药皮多糖超声辅助提取工艺及其抗氧化活性

    Institute of Scientific and Technical Information of China (English)

    马艳弘; 殷剑美; 魏建明; 李亚辉; 张宏志; 张立群

    2016-01-01

    This present study focused on extraction of polysaccharides from Chinese yam peel by ultrasonic as-sisted method, and the antioxidant activity of polysaccharides from Chinese yam peels was also evaluated. The extraction conditions of polysaccharides were optimized using response surface methodology on the basis of sin-gle factor experiment. The DPPH radical scavenging capacities and hydroxyl radical(·OH) scavenging capaci-ties were measured. Results showed that the optimal parameiers of ultrasonic extractin were solid/liquid ratio 1/20 (g/mL), ultrasonic time 110 min, extraction time 40 min, extraction temperature 70℃. Under these condi-tions, the polysaccharides extraction rate reached 185.12 mg/g, increased by 48.89%compared with the con-trol without ultrasonic assisted extration. The polysaccharides obtained possessed potent antioxidant activity, and the antioxidant activity of polysaccharides from Chinese yam peel was found in does dependent manner. Un-der the concentration of 3.2 mg/mL, the radical scavenging rate on DPPH and·OH were 87.67%, 71.20%, re-spectively.%研究山药皮多糖的超声辅助提取工艺及其抗氧化活性。结果显示:山药皮多糖的最佳提取条件为料液比1∶20(g/mL)、超声时间110 min、提取时间40 min,提取温度70℃,在此条件下,多糖的提取率为185.12 mg/g,与未超声波辅助提取对照试验相比提高了48.89%,所提多糖具有较好的抗氧化活性,且在一定浓度范围内,其抗氧化能力与多糖质量浓度呈现一定的剂量效应关系。当质量浓度为3.2 mg/mL时其对DPPH自由基和羟自由基清除率分别达到87.67%、71.20%。

  16. 中华芦荟多糖的提取·精制和含量测定研究%Extraction,Purification and Content Determination of Polysaccharides in Aloe vera L. var chinensis

    Institute of Scientific and Technical Information of China (English)

    丁晨; 郭彬歆; 徐琛

    2012-01-01

    [Objective] To optimize the extraction technology of poly sac charides from Aloe vera L. var chinensis, compare several purification methods and establish a method for determining the content of polysaccharides. [ Method] With the yield and content of polysaccharides as the evaluation criteria, the extraction process of polysaccharides was optimized by orthogonal experiment. Three polysaccharides purification methods, including dialysis, macroporous resin AB-8 absorption and activated carbon absorption, were compared. The polysaccharides content was detected colorimetrically by the phenol-sulfuric acid method after the conversion coefficient of Aloe vera L. vat ckinensis polysaeeharides to glu-cose was obtained. [Result] The optimal process conditions for extraction were obtained by orthogonal experiment and the optimal parameters were as follows: the solid-liquid ratio of 1:10 g/ml, twice of extraction, and 3 h of extraction time. The contents of polysaccharide purified by the three methods were shown as dialysis > macroporous resin AB-8 > activated carbon; while the yield of polysaccharides was shown as activated carbon > dialysis > macroporous resin AB-8; the conversion coefficient of polysaccharide to glucose was determined to be 1. 32, the average recovery was 98.4% with a relative standard deviation of 2.36%. [Conclusion] The optimized extraction process is economical, simple, stable and practical, the dialysis method is simple, rapid and cheap to be used for purifying the polysaccharides. The phenol-sulfuric acid method is simple and accurate with good reproducibility.%[目的]优化中华芦荟多糖的提取工艺,比较几种精制方法,并建立多糖含量的测定方法.[方法]以多糖得率和含量为评价指标,采用正交试验法对多糖提取工艺进行研究,并比较透析法、AB-8型大孔树脂吸附法和活性炭吸附法3种多糖精制方法;采用芦荟多糖标准品测得芦荟多糖对葡萄糖的换算因子后,用苯酚-

  17. Polysaccharide Degradation

    Science.gov (United States)

    Stone, Bruce A.; Svensson, Birte; Collins, Michelle E.; Rastall, Robert A.

    An overview of current and potential enzymes used to degrade polysaccharides is presented. Such depolymerases are comprised of glycoside hydrolases, glycosyl transferases, phosphorylases and lyases, and their classification, active sites and action patterns are discussed. Additionally, the mechanisms that these enzymes use to cleave glycosidic linkages is reviewed as are inhibitors of depolymerase activity; reagents which react with amino acid residues, glycoside derivatives, transition state inhibitors and proteinaceous inhibitors. The characterization of various enzymes of microbial, animal or plant origin has led to their widespread use in the production of important oligosaccharides which can be incorporated into food stuffs. Sources of polysaccharides of particular interest in this chapter are those from plants and include inulin, dextran, xylan and pectin, as their hydrolysis products are purported to be functional foods in the context of gastrointestinal health. An alternative use of degraded polysaccharides is in the treatment of disease. The possibility exists to treat bacterial exopolysaccharide with lyases from bacteriophage to produce oligosaccharides exhibiting bioactive sequences. Although this area is currently in its infancy the knowledge is available to investigate further.

  18. Antioxidant and Anti-Inflammatory Properties of an Extract Rich in Polysaccharides of the Mushroom Polyporus dermoporus

    Directory of Open Access Journals (Sweden)

    Celina Maria P. Guerra Dore

    2014-11-01

    Full Text Available Polyporus dermoporus mushroom, native to Brazil, is produced under natural conditions in the unexplored reserve of Mata da Estrela-Rio Grande do Norte-RN. These mushrooms were delipidated with chloroform:methanol (2:1 v/v, extracted with water at 100 °C, and fractionated with ethanol (one and three volumes and then centrifuged. The ethanol precipitation showed a high total sugar level of 64.8% and 1% of protein. This precipitate contained a high glucan level, characterized by chemical methods and by NMR of 13C and 1H and spectroscopy. The 13C NMR spectrum of these mushroom extracts showed the presence of β-glucose by a signal at 103.25 ppm. Studies with these glucans were made to elucidate antioxidant and anti-inflammatory activities. This extract of glucans inhibited the lipid peroxidation (42.9% and superoxide radicals (83.3% at 67 μg/mL. However, the inhibition of hydroxyl radical by the extract of this mushroom was 96% at 267 μg/mL. The action of this extract on induced pleurisy showed a 92.5% and 68.7% reduction in polymorphonuclears cells and nitric oxide, respectively, at 30 mg/kg. The glucans reduced the croton oil-induced ear edema by 65.6% at 30 mg/kg.

  19. Modeling of extraction process of crude polysaccharides from Basil seeds (Ocimum basilicum l.) as affected by process variables.

    Science.gov (United States)

    Salehi, Fakhreddin; Kashaninejad, Mahdi; Tadayyon, Ali; Arabameri, Fatemeh

    2015-08-01

    Basil seed (Ocimum basilicum L.) has practical amounts of gum with good functional properties. In this work, extraction of gum from Basil seed was studied. Effect of pH, temperature and water/seed ratio on the kinetic and thermodynamic parameters; entropy, enthalpy and free energy of extraction were investigated. The maximum gum yield was 17.95 % at 50 °C for pH=7 and water/seed ratio 30:1. In this study, the experimental data were fitted to a mathematical model of mass transfer and equations constants were obtained. The kinetic of Basil seed gum extraction was found to be a first order mass transfer model. Statistical results indicated that the model used in this study will be able to predict the gum extraction from Basil seed adequately. It also found that ΔH and ΔS were positive and ΔG was negative indicating that the extraction process was spontaneous, irreversible and endothermic. The ΔH, ΔS and ΔG values were 0.26-7.87 kJ/mol, 8.12-33.2 J/mol K and 1.62-4.42 kJ/mol, respectively.

  20. Cell wall polysaccharides in black currants and bilberries-characterisation in berries, juice, and press cake

    NARCIS (Netherlands)

    Hilz, H.; Bakx, E.J.; Schols, H.A.; Voragen, A.G.J.

    2005-01-01

    Cell wall polysaccharides from black currants and bilberries were characterised in three approaches. First, compositions of skin, pulp, and seeds show the distribution of polysaccharides over these tissues. A sequential extraction of cell wall material with different aqueous extractants informs

  1. 微波辅助法萃取马齿苋多糖的工艺优化%Process Optimization of Microwave-assisted Extraction of Polysaccharide from Portulaca oleracea

    Institute of Scientific and Technical Information of China (English)

    梁彦

    2012-01-01

    In order to optimize the microwave-assisted extraction process of polysaccharide from P. oleracea, a single-factor test and an orthogonal test were conducted to explore the effects of liquid-solid ratio, microwave processing time and microwave power on the polysaccharide extraction rate. The results showed that the optimum conditions for microwave-assisted extraction of polysaccharide from P. oleracea were as follows: liquid-solid ratio 35 ! 1 (mL ! g), microwave processing time 15 min and microwave power 540 W. The extraction rate could be up to 11. 69% under these conditions. Compared with water extraction, microwave-assisted extraction saved extraction time (40 min) and improved the extraction rate (8.69%).%为了优化微波辅助法萃取马齿苋多糖工艺,采用单因素试验和正交试验优化工艺条件研究了液料比、微波处理时间、微波功率对马齿苋多糖萃取率的影响.结果表明:微波辅助法萃取马齿苋多糖最佳工艺条件为液料比35∶1(mL∶g),微波处理时间15 min,微波功率540W,此时萃取率为11.69%.与水提法相比(40 min,萃取率8.69%),微波辅助提取法节省了萃取时间,提高了萃取率.

  2. 响应面法优化碱溶性龙胆多糖的提取研究%Optimization Extraction of Alkali-solubility Polysaccharide from Gentiana scabra Bunge by Response Surface Methodology

    Institute of Scientific and Technical Information of China (English)

    温钢; 冯凯

    2015-01-01

    For the further development of the alkali‐soluble polysaccharide extraction ,response surface method‐ology (RSM) was used to optimize the extraction conditions of alkaline solubility polysaccharides from the Gentiana scabra Bunge .The method of alkali extraction and alcohol sink was used to extract polysaccharides . The effect of extraction times ,extraction temperature ,extraction time ,ratio of water to raw material ,and NaOH concentration on the yield of Gentiana scabra Bunge polysaccharides were investigated .The results showed that combining single factor test and response surface methodology ,the optimum extraction conditions were chosen as follows:extraction temperature was 74℃ ,ratio of water to raw material was 1∶25 ,extraction time was 1 .8 h ,one time extraction and the NaOH concentration was 9% .Under the optimize conditions ,the GSP predicted value of yield was 4 .4% ,and the GSP experimental yield was 4 .32 ± 0 .124% ,the results were reasonable with expectation .%为深入开发提取碱溶性多糖,采用响应面法(RSM )即碱提醇沉法对龙胆多糖进行提取,研究提取次数、提取温度、提取时间、液料比、碱浓度等因素对多糖提取率的影响。结果表明:在单因素试验基础上,结合响应面分析确定了提取碱溶性龙胆多糖的最适工艺条件为,提取温度74℃,料液比1∶25,提取时间1.8h,提取次数为1次,碱浓度为9%。模型预测的碱溶性龙胆多糖的提取率为4.4%,在此最佳提取参数下,碱溶性龙胆多糖的试验提取率为(4.32±0.124)%,与预期值相符。

  3. 超声波破壁提取葡萄酒酵母泥中多糖的研究%Study on extraction of polysaccharide by ultrasonic dilapidating walls from waste wine yeast slurry

    Institute of Scientific and Technical Information of China (English)

    胡云峰; 陈君然; 胡晗艳; 崔瀚元; 杨秋月

    2013-01-01

    Objective To establish a method for the extraction of polysaccharide of waste wine yeast slurry by ultrasonic method. Methods On the basis of single factor experiments, the effects of operating con-ditions, such as yeast concentration, ultrasonic-assisted extraction temperature, and ultrasonic-assisted extrac-tion time, were analyzed by response surface methodology. Results The optimized conditions of ultrason-ic-assisted extraction are as following: yeast concentration 9.10%, ultrasonic-assisted extraction temperature 65.42 ℃, and ultrasonic-assisted extraction time 132.97 min. Under the optimized conditions, the extraction yield of yeast polysaccharide was 1.85%. The optimum technological condition was validated. the actual ex-traction yield of yeast polysaccharide was 1.86%. It showed that the result was stable. Conclusion The ultra-sonic-assisted extraction method has a high extraction rate in extracting the polysaccharide of waste wine yeast slurry. The method is simple and practical.%目的:利用超声波法提取葡萄酒酵母泥中多糖。方法研究了酵母浓度、超声温度、超声时间对葡萄酒酵母泥中多糖得率的影响,并采用响应面分析法对葡萄酒酵母泥中多糖提取工艺进行优化设计。结果超声波法提取葡萄酒酵母泥中多糖的最佳条件为:酵母浓度9.10%,超声温度为65.42℃,超声时间为132.97 min。最终酵母多糖得率为1.85%,对最佳工艺条件进行验证,酵母多糖实际得率为1.86%,结果重复性较好。结论超声波辅助提取葡萄酒酵母泥中的多糖,工艺简便,多糖得率较高,具有实际的应用价值。

  4. Effects of Black Hoof Medicinal Mushroom, Phellinus linteus (Agaricomycetes), Polysaccharide Extract in Streptozotocin-Induced Diabetic Rats.

    Science.gov (United States)

    Yamac, Mustafa; Zeytinoglu, Melih; Senturk, Hakan; Kartkaya, Kazim; Kanbak, Gungor; Bayramoglu, Gokhan; Oglakci, Aysegul; Van Griensven, Leo J L D

    2016-01-01

    In this article we report the healing effects of a Phellinus linteus fruiting body hot water extract (PLE) in streptozotocin (STZ)-induced diabetic rats. PLE was given before and after STZ. The preprotective, protective, and postprotective effects of PLE on STZ-induced oxidative stress were studied using biochemical (caspase 3 activity, cytosolic-to-lysosomal ratio of cathepsin B and L, DNA fragmentation levels), ordinary histological and immuno-histochemical investigation parameters. Following oral administration of PLE after STZ application, the serum glucose concentration significantly decreased up to 41.13% compared with the control group (P Linteus hot water extract on STZ-induced diabetes.

  5. Effects of black hoof medicinal mushroom, phellinus linteus (Agaricomycetes), polysaccharide extract in streptozotocin-induced diabetic rats

    NARCIS (Netherlands)

    Yamaç, Mustafa; Zeytinoğlu, Melih; Şentürk, Hakan; Kartkaya, Kazim; Kanbak, Göngör; Bayramoğlu, Gökhan; Oğlakci, Ayşegül; Griensven, van Leo J.L.D.

    2016-01-01

    In this article we report the healing effects of a Phellinus linteus fruiting body hot water extract (PLE) in streptozotocin (STZ)–induced diabetic rats. PLE was given before and after STZ. The preprotective, protective, and postprotective effects of PLE on STZ-induced oxidative stress were studi

  6. Structural characteristics of pectic polysaccharides from olive fruit (Olea europaea cv moraiolo) in relation to processing for oil extraction

    NARCIS (Netherlands)

    Vierhuis, E.; Korver, M.; Schols, H.A.; Voragen, A.G.J.

    2002-01-01

    In the olive oil industry technical enzyme preparations are used as processing aids to improve the extraction yield and product quality. In order to obtain more insight in the mechanisms by which these enzyme preparations lead to a higher yield and a better quality, the effect of these preparations

  7. Effects of black hoof medicinal mushroom, phellinus linteus (Agaricomycetes), polysaccharide extract in streptozotocin-induced diabetic rats

    NARCIS (Netherlands)

    Yamaç, Mustafa; Zeytinoğlu, Melih; Şentürk, Hakan; Kartkaya, Kazim; Kanbak, Göngör; Bayramoğlu, Gökhan; Oğlakci, Ayşegül; Griensven, van Leo J.L.D.

    2016-01-01

    In this article we report the healing effects of a Phellinus linteus fruiting body hot water extract (PLE) in streptozotocin (STZ)–induced diabetic rats. PLE was given before and after STZ. The preprotective, protective, and postprotective effects of PLE on STZ-induced oxidative stress were

  8. 蒙药瞿麦中多糖超声提取工艺研究及含量测定%Extraction optimization and content determination of polysaccharide in Mongolian medicine Dianthus superbus L.

    Institute of Scientific and Technical Information of China (English)

    陈建平; 王金辉; 汤化琪; 盛华; 杜艳青

    2013-01-01

    Objective To explore the extraction and determination of polysaccharide in the Mongolian medicine Dianthus superbus L.. Methods Phenol-sulfuric acid method and UV-PDA spectrophotometer were used to determine polysaccharide, which was used as the evaluation index to determine the optimum technological conditions of polysaccharide extraction in Dianthus superbus L. by a single factor and orthogonal experiments. Results The best ultrasonic extraction of polysaccharide in Dianthus superbus L. was as follows: solid-liquid ratio at 1 : 20, 60 ℃ , ultrasonic extraction 30 min, extracting twice. The order of the 4 factors was extraction temperature > extraction time > number of extraction > solid-liquid ratio. The polysaccharide content measured in Dianthus superbus L. was 5.463%; the average recovery rate was 98.6%, RSD = 1.5% (n = 6). Conclusion The above extraction is the best ultrasonic extraction of polysaccharide in Dianthus superbus L.. UV-Vis spectrophotometric method is simple, rapid, sensitive, accurate and reliable, which can be used to determine polysaccharide in Mongolian medicine Dianthus superbus L..%目的 研究瞿麦中多糖的超声提取方法及其含量测定的方法.方法 采用苯酚-硫酸法显色,并以紫外-可见分光光度计测定瞿麦中多糖含量,以其作为评价指标,先后通过单因素试验和正交试验来确定提取瞿麦多糖的最佳工艺条件.结果 超声法提取瞿麦中多糖的最佳工艺为料液比为1∶20,温度60℃,超声时间为30 min,提取2次.各因素影响顺序为提取温度>提取时间>提取次数>料液比,测得瞿麦中多糖含量为5.463%,平均回收率为98.6%,RSD=1.5%(n=6).结论 以上工艺为运用超声技术从瞿麦中提取出多糖的最佳工艺.采用紫外可见分光光度法测定瞿麦中多糖含量的方法简便、快速、灵敏,结果准确可靠,可作瞿麦中多糖的含量测定方法.

  9. Moisturizing effect of serine-loaded solid lipid nanoparticles and polysaccharide-rich extract of root Phragmites communis incorporated in hydrogel bases.

    Science.gov (United States)

    Barua, Sonia; Kim, Hyeongmin; Hong, Seong-Chul; Yoo, Seung-Yup; Shin, Dohyun; Lee, Chung-Lyol; Na, Seon-Jeong; Kim, Yeong Hyo; Jo, Kanghee; Yun, Gyiae; Kim, Joong-Hark; Sohn, Uy Dong; Lee, Jaehwi

    2017-02-01

    This study evaluated the moisturizing effect of serine-loaded solid lipid nanoparticles (serine-SLN) and polysaccharide-rich reed (Phragmites communis) root extract (RRE) incorporated in hydrogel bases. The hydrogels with serine-SLN and/or RRE were carefully applied on the volar forearm of human volunteers. Their moisturizing efficacy was evaluated by monitoring conductance values using a skin surface hygrometer. The values of the area under the normalized conductance-time curve (AUCC) were developed and compared as a parameter for the water holding capacity of the skin. Hydrogels with serine-SLN did not significantly moisturize the skin, while hydrogel containing 0.25% RRE produced a significant increase in the moisture content of the skin. However, adding more than 0.25% of RRE into the hydrogel base decreased the moisturizing effect due to the marked reduction of viscosity. Significantly enhanced moisturizing effect was observed with the hydrogel containing 0.25% RRE and 3% serine-SLN, with AUCC increased 2.21 times compared to than blank hydrogel. The results imply that effective delivery of serine into the skin is possible using lipid-based nanocarriers and RRE, which could be a promising strategy to moisturize the skin effectively.

  10. 中性蛋白酶法提取富硒羊肚菌菌丝体多糖的研究%Study on the Extraction of Mycelium Polysaccharides from Selenium-rich Morchella esculenta with Neutral Proteinase Method

    Institute of Scientific and Technical Information of China (English)

    孙玉军; 李正鹏; 马忠友; 王松华

    2012-01-01

    Objective:To study the extraction conditions of mycelium polysaccharides from selenium-rich Morchella esculenta with neutral proteinase for increasing of yield. Methods:On the basis of single factor tests, orthogonal experiment design was applied to analyze the influence of factors including enzyme dosage, enzymolysis time, enzymolysis temperature and material/liquid ratio on the extraction rate of the polysaccharides. Results:The optimal extraction conditions of mycelium polysaccharides from selenium-rich Morchella esculenta was as follows; enzyme dosage was 1.5% ,enzymolysis time was 2 h,enzymolysis temperature was 40 ℃,and material/liquid ratio was 1 :15. The extraction rate of polysaccharide was 11. 26 % under the extraction conditions. Conclusion:The process is simple, stable and practicable, and can be used for the extraction of mycelium polysaccharides from selenium-rich Morchella esculenta.%目的:研究中性蛋白酶法提取富硒羊肚菌菌丝体多糖的条件,以提高菌丝体多糖的得率.方法:在单因素试验的基础上,进行正交试验设计,考察加酶量、酶解时间、酶解温度、料液比等因素对多糖提取率的影响.结果:确定了中性蛋白酶法提取富硒羊肚菌菌丝体多糖的最佳工艺:加酶量为1.5%,提取时间为2h,酶解温度为40℃,料液比为1∶15,在此条件下,提取率可达11.26%.结论:本工艺简单、稳定、可行,适用于富硒羊肚菌菌丝体多糖的提取.

  11. 响应曲面法优化玉米须多糖提取工艺的研究%Study on Extraction Process of Polysaccharide of Corn Silk Optimized by Response Surface Methodology

    Institute of Scientific and Technical Information of China (English)

    王艺帷; 唐艳花; 吴娜; 王静

    2015-01-01

    This research makes the polysaccharide extraction of corn silk by means of microwave as-sisted extraction and the ethanol precipitation method.Based on the single factor experiment results,and ac-cording to the experimental design principle of Box -Behnken Center,it designs the experiment of three fac-tors three levels.The software of Design -Expert is adopted to make response surface analysis,thus to build the regression equation of the polysaccharide extraction of corn silk.By checking,this regression equation is reasonable and reliable.It can better predict the extraction rate of the Polysaccharide extraction of corn silk. The optimum condition is determined by the method of response surface for the material liquid ratio 1∶8(g/mL).The extraction temperature is 93℃.The extraction time is 3 min.It is verified by experiment that the extraction rate of Polysaccharide extraction of corn silk is 1.13%,and the relative error with prediction is 0.88%.%采用微波辅助,乙醇沉淀法提取玉米须中的多糖,在单因素实验基础上,利用 Box -Behnken 实验设计原理,设计3因素3水平实验,采用 Design -Expert 进行响应面分析,建立玉米须多糖提取的回归方程。经检验该回归方程能够较好的预测玉米须多糖的提取率。经响应面优化法确定的最佳工艺条件为:料液比1∶8(g/mL),提取温度93℃、提取时间30 min。经过实验验证,玉米须多糖的提取率为1.13%,与预测值的相对误差为0.88%。

  12. 缓冲溶液浸提天麻多糖工艺的优化及抗氧化性研究%Study on citrate buffer assisted extraction of polysaccharide from Gastrodia elata and its antioxidant activity

    Institute of Scientific and Technical Information of China (English)

    曹小燕; 杨海涛

    2016-01-01

    Taking Gastrodia elata from Qinba area as raw material,citrate buffer solution at pH 6 as the extraction solvent,the extraction and antioxidant activities of polysaccharides from Gastrodia elata by ul-trasonic were studied.The results showed that the optimum extraction technology of Gastrodia elata poly-saccharides were as follows:extraction temperature 50 ℃,solid-liquid ratio of 1∶40 g/mL,ultrasonic ex-traction time of 30 min,extract power 300 W,citrate buffer solution pH 6.0 as the extraction agent,under this condition,the extraction yield of polysaccharide was 36.21%,which was 20% higher than traditional extraction.Scavenging free radicals IC50 values for O2 -·,·OH and ABTS +· were 0.46,0.76 mg/mL and 6.7 μg/mL respectively,which was superior to the conventional antioxidants of ascorbic acid.The study proved that polysaccharides from Gastrodia elata has good reducing capacity and scavenging radical activity.%以秦巴山区天麻为原料,pH 6柠檬酸盐酸性缓冲溶液为浸提溶剂,研究超声辅助浸提天麻多糖的工艺条件,并考察其还原能力和清除羟基自由基、超氧阴离子自由基和 ABTS 自由基正离子的能力。结果表明,天麻多糖的最佳提取工艺为:超声温度50℃,料液比1∶40 g/mL,提取时间30 min,超声功率300 W,柠檬酸盐缓冲溶液 pH 6。该工艺条件下,天麻多糖的提取率为36.21%,与传统水提法相比,提高了20%。天麻多糖清除 O2-·、·OH、ABTS +·的 IC50值分别为0.46,0.76 mg/mL 和6.7μg/mL,优于同浓度条件下抗氧化剂 VC 溶液。

  13. 响应面法优化提取灰树花碱溶性多糖%Optimized extraction of alkali-soluble polysaccharide of Grifola frondosa by response surface method

    Institute of Scientific and Technical Information of China (English)

    柴丽; 侯红漫; 张公亮

    2013-01-01

    利用试验设计软件Design-Expert,通过二次回归设计得到灰树花碱提多糖提取率与料液比、提取时间、氢氧化钠浓度的回归模型,该模型能较好地预测灰树花碱提多糖的提取率.探讨了影响多糖提取率的关键因素及其相互作用,得到优化提取工艺参数∶料液比1∶66,提取时间33 min,氢氧化钠浓度0.29 mol/L.在此条件下,碱溶性多糖提取率为0.70%.%The influence of liquid-solid ratio, extraction time and sodium hydroxide concentration on extraction ratio of alkali extraction Grifola frondosa polysaccharide were studied by quadratic regression orthogonal design. These three parameters were analyzed and optimized by Design-Expert experimental software through the regression model. The optimized extraction parameters were obtained as follows: liquid-solid ratio 66 : 1, extraction time 33 min, sodium hydroxide concentration 0. 29 mol/L. Under that condition, the extraction ratio of alkali-soluble polysaccharide of Grifola frondosa reached 0. 70%.

  14. Extraction of spirulina platensis polysaccharide by ethanol/(NH4)2SO4 aqueous two-phase system%乙醇/硫酸铵双水相体系萃取螺旋藻多糖的研究

    Institute of Scientific and Technical Information of China (English)

    刘杨; 冯元琦; 陈美欣; 吴景淳; 黄海根

    2012-01-01

    Extraction of spirulina platensis polysaccharide by ethanol/( NH4 ) 2SO4 aqueous two-phase system(ATPS)was investigated. To remove and recycle the proteins, salting-out precipitation protein was carried out after spirulina platensis cells disruption. Spirulina platensis polysaccharide was extracted by traditional hot water diffusion and the polysaccharide dissolution rate reached 38.44 ± 1. 12mg/g spirulina platensis powder. Spirulina platensis polysaccharide was purified deeply by ethanol/ (NH4)2SO4 ATPS. The yield and enrichment factor of the polysaccharide were 84. 5±1. 45% and 6. 2 respectively,on the condition that the tie line length, volume ratio and pH of ATPS were 42. 9,1. 05 and 7. 0, respectively. The results indicated that spirulina platensis polysaccharide would be purified by a simple, efficient and low cost extraction technology using inexpensive ethanol/ (NH4)2SO4 ATPS.%考察了低分子有机溶剂与无机盐-乙醇/硫酸铵双水相体系萃取螺旋藻多糖的可行性及影响因素.研究结果表明:为除去并有效回收螺旋藻细胞中的蛋白质成分,在藻细胞破碎后进行盐析沉淀蛋白质,再采用传统的热水浸提法,可得到螺旋藻多糖的溶出率为38.44±1.12mg/g干燥粉;通过乙醇/硫酸铵双水相体系的萃取分配,在w(乙醇)=19%,w(硫酸铵)=27.5%(即双水相体系系线长度TLL =42.9),体系相比VR=1.05,pH=7.0时,螺旋藻多糖的收率可达84.5±1.45%,富集因子可达6.2.该研究结果表明廉价的乙醇/硫酸铵双水相萃取螺旋藻多糖将有望开发成为一条简洁、高效、低成本的螺旋藻多糖分离提取工艺.

  15. 薏苡仁多糖的提取及其对油脂的抗氧化作用研究%Study on Extraction and Antioxidant Activity of Polysaccharide from Coix Seed

    Institute of Scientific and Technical Information of China (English)

    张洋婷; 马洪波; 宋春梅; 葛红娟; 王舒然

    2015-01-01

    Microwave-assisted extraction was applied to extract polysaccharide from coix seeds. Single factor and orthogonal experiment were applied to optimize the extraction conditions. The result showed that the optimal extraction conditions of extraction time , microwave power and liquid-to-material ratio were 4 min , 320 W and 1:30(g/mL), respectively.Under these conditions,the extraction rate of polysaccharide was 10.52%. Result of antioxidation experiment in oil suggested that polysaccharide from coix seeds had antioxidant effects on lard oil , sesame oil, soybean oil and sunflower oil, and in the order as sesame oil>soybean oil>sunflower oil>lard oil.%用微波辅助法提取薏苡仁中多糖,采用单因素试验和正交试验优化提取条件.结果表明,最佳提取工艺条件为:提取时间4 min、微波功率320 W、液料比1:30(g/mL),在此条件下薏苡仁粗多糖提取率可达10.52%.油脂抗氧化试验表明,薏苡仁多糖对猪油、芝麻油、豆油和葵花籽油均具有抗氧化作用,且抗氧化作用的大小顺序为芝麻油>豆油>葵花籽油>猪油.

  16. 溪黄草多糖的微波提取及其抗氧化性研究%Study on the Microwave Extraction and Antioxidant Activities of Polysaccharides from Rabdosia serra(Maxim.) Hara

    Institute of Scientific and Technical Information of China (English)

    许丽丽; 沈楚椰

    2015-01-01

    研究微波法提取溪黄草多糖的最佳工艺条件并测定其抗氧化活性。通过单因素试验和正交试验优化提取条件,对溪黄草多糖还原能力及清除H2O2能力进行测定。结果表明,溪黄草多糖的最佳提取条件为:微波功率480 W、微波时间75 s、料液比1∶35(g/mL)、浸提次数2次,此条件下多糖得率为12.07 mg/g,溪黄草多糖具有良好的抗氧化活性。该法操作便捷,耗时少,条件温和,为溪黄草资源开发利用提供理论参考。%The optimum extraction conditions and antioxidant activities of polysaccharides from Rabdosia serra (Maxim.) Hara was studied. The extraction conditions were optimized by the single factor experiments and orthogonal experiments. Then the antioxidant activities of polysaccharides were determined by the experiments of reducing capacity and removing H2O2 capacity. Results indicated that the best extracting conditions were as follows: microwave power 480 W, treatment time 75 s,ratio of material to solvent was 1 ∶ 35 (g/mL) and extraction 2 times. Under this condition, the extraction rate of polysaccharides was 12.07 mg/g. The extracts showed obvious antioxidant activities. This method was simple, time-saving, under mild conditions. It provides a scientific basis for the exploitation and utilization of Rabdosia serra(Maxim.) Hara.

  17. Optimization of Ultrasonic-assisted Extraction Process of Polysaccharides from Truffles by Response Surface Methodology%响应面法优化块菌多糖的超声波辅助提取工艺

    Institute of Scientific and Technical Information of China (English)

    蒲彪; 岳金玫; 陈安均; 刘兴艳

    2013-01-01

    利用响应面分析法对超声波辅助提取块茵多糖的工艺进行优化.在单因素试验的基础上,以超声时间、功率、温度和液料比为自变量,以多糖提取率和多糖含量共为响应值,进行Box-Behnken中心组合试验,回归分析确定最优工艺条件.分析各个因素的显著性和交互作用后,得出最优工艺条件为:超声功率140W、处理时间36min、处理温度50℃、液料比40∶1,多糖理论提取率为18.42%,实际提取率为18.15%,相对误差为1.47%;多糖理论含量为74.70%,实际含量73.89%,相对误差为1.08%.%To optimize the extraction process of Truffles polysaccharides,the response surface methodology (RSM) was adopted.Based on the single factor experiment the mathematical regression model was established about the dependen' variable (extraction yield of Truffles polysaccharides and polysaccharides content) and independent variables (ultrasonic time,ultrasonic power,ultrasonic temperature and liquid-solid ratio).The factors influencing the process parameters were determined by regression analysis,and the results showed that the optimum conditions for the extraction were as follows:ultrasonic power was 140W,ultrasonic time was 36min,ultrasonic temperature was 50℃ and liquid-solid ratio was 40:1.The predictive maximum yield of polysaccharides extraction was 18.42% and the polysaccharides content was 74.70%,under the optimized process conditions,the actual extraction was 18.15% and the relative error was 1.47%,the actual content was 73.89% and the relative error was 1.08%.

  18. 纤维素酶酶解法提取怀山药多糖工艺研究%Study on Extraction of Polysaccharide from Dioscorea Opposita Thunb by Enzymatic Hydrolysis of Cellulase

    Institute of Scientific and Technical Information of China (English)

    李培

    2015-01-01

    The Polysaccharide was extracted from Dioscorea opposita Thunb by enzymatic hydrolysis of Cellulase .First,the effects of the ratio of material and water ,the amount of the enzyme ,the time of enzyme hydrolysis , and the temperature of enzyme hydrolysis on the extraction rate of Polysaccharide from Di-oscorea opposita Thunb were studied by single factor experiment .Then the extraction process of Polysac-charide from Dioscorea opposita Thunb was optimized by orthogonal test .The results showed that the order of the primary and secondary factors affecting the extraction rate of Polysaccharides from Dioscorea opposi -ta Thunb was in order:the amount of the enzyme ,the time of enzyme hydrolysis ,the temperature of en-zyme hydrolysis ,the ratio of material and water .The optimal extraction conditions were as follows:the a-mount of the enzyme 3%, the time of enzyme hydrolysis 75 min,the temperature of enzyme hydrolysis 60℃,the ratio of material and water 1:30 .The Polysaccharide yield of 15.78% was achieved under these conditions .%以怀山药为材料,采用纤维素酶酶解,提取其活性成分多糖,先通过单因素实验考察了料水比、酶添加量、酶解时间,酶解温度对怀山药多糖提取得率的影响;然后通过正交试验对怀山药多糖的酶解提取工艺进行了优化,结果表明各因素对怀山药多糖提取率影响的主次顺序依次为:酶添加量、酶解时间、酶解温度、料水比;其最优提取工艺条件为:酶添加量3%,酶解时间75 min,酶解温度60 ℃,料水比1:30,在此最优工艺条件下,怀山药多糖的提取率为15.78%.

  19. 超声提取甜菜多糖对小鼠免疫功能影响的研究%Preliminary Study on Effects of Beetroot Polysaccharide Extracted by Ultrasonic Wave on Immune Function of Mice

    Institute of Scientific and Technical Information of China (English)

    常永志; 包晓玮; 杨晓君; 何江梅; 艾热提

    2011-01-01

    [目的]研究甜菜多糖对正常小鼠免疫功能调节作用的影响.[方法]利用超声提取法得到80%的乙醇粗提物,通过紫外光谱测定甜菜提取物多糖含量为85.82%.将甜菜多糖配成(50 mg/(kg·d)),实验小鼠经胃灌注甜菜多糖,连续7d后处死,测定给药后小鼠脾脏及胸腺系数和腹腔巨噬细胞的吞噬功能.[结果]甜菜多糖能提高小鼠免疫器官重量、巨噬细胞的吞噬功能.[结论]甜菜多糖具有免疫调节活性作用.%[Objective] The purpose of this study was to investigate the effects of Beetroot polysaccharide on immune function of mice. [Method] 80% alcohol etude extract was obtained by ultrasonic wave and the determination of content of the beetroot polysaccharide via ultraviolet spectrophotometry was 85. 82%. Hie experimental mice were infused via stomach with beetroot polysaccharide prepared for 50mg/kg·d, one time a day, and the experiment lasted 7 days. Tne mouse vivo immune function tests are carried out on the 8th day through the determination of their indices of thymus and spleen and the phagocytic activity of abdomen macraphages of mice. [Result] Beetroot polysaccharide can increase the weight of the immune organs and phagocytotic function of the macrophages. [Conclusion] Beetroot polysaccharide can regulate the immune functions of mice.

  20. 浒苔多糖的酶法提取、纯化及初步结构鉴定%Study on the Extraction, Purification and Structural Characterization of Polysaccharide from Enteromorpha

    Institute of Scientific and Technical Information of China (English)

    吕海涛; 肖宝石; 高玉杰

    2013-01-01

    Polysaccharide was extracted from Enteromorpha by hot water-papain. The crude Enteromorpha polysaccharide was separated and purified by DEAE ion-exchange cellulose chromatography and four kinds of polysaccharide components were obtained. The identification of purification with Sephadex G -100 gel chromatography implied that EP1 was homogeneous polysaccharide. From the result of FTIR, EP1 had the polysaccharide characteristic absorption peak and the sulfur acid radical existence. There were no nucleic acid and protein absorbance at 260 nm and 280 nm on the ultraviolet spectrum. The monosaccharide composition of EP1 was analyzed by HPLC, which was Man∶Rha∶Glu∶Xyl=0.09∶1.0∶3.1∶0.29.%  采用热水-木瓜蛋白酶法从浒苔中提取多糖,用DEAE-52纤维柱对浒苔粗多糖分离,得到4个多糖EP1、EP2、EP3和EP4组分。经Sephadex G-100凝胶柱进行纯度鉴定,表明EP1是均一多糖,红外光谱表明EP1具有多糖的特征吸收峰及硫酸基存在,紫外光谱表明EP1在260 nm和280 nm没有核酸和蛋白质的特征吸收峰,HPLC法分析表明EP1的单糖组成为:甘露糖∶鼠李糖∶葡萄糖∶木糖=0.09∶1.0∶3.1∶0.29。

  1. Optimization of Extraction Process by Using Response Surface Methodology and Antioxidant Activity of Polysaccharide from Yellow Mealworm%黄粉虫多糖响应面法提取及抗氧化活性

    Institute of Scientific and Technical Information of China (English)

    何钊; 冯颖; 孙龙; 陈智勇; 陈晓鸣

    2011-01-01

    In this studyrespone surface analysis methodology (RSM) was applied to optimize the extraction conditions of polysaccharide from yellow mealworm ( Terebrio molitor L. ). Antioxidant capability of the polysaccharide was studied in vitro. The optimal range of key factors were studied by regression analysis and listed as follows:temperature 90℃ time 158 min;the ratio of liquid to solid 12. With the optimum conditionsthe yield of polysaccharide was achieved at 1. 94% (the predicted yield was 1. 99%). The polysaccharide from yellow mealworm was constituted glucose mainlymannose and galactose. The results also demonstrated that yellow mealworm polysaccharide has antioxidant activity in vitrowhich means it could be explored as a potential natural antioxidant.%采用响应面分析法研究黄粉虫多糖提取的工艺条件,同时检测提取多糖的抗氧化活性.通过对关键影响因子的最佳水平范围的研究,依据回归分析确定了黄粉虫多糖最佳提取条件为:提取温度90℃、液固比12、提取时间158 min,在此条件下黄粉虫多糖提取率的理论值为1.99%,实测值为1.94%.分析表明,黄粉虫多糖主要由葡萄糖组成,并含少量甘露糖和半乳糖.体外抗氧化试验显示,黄粉虫多糖具有一定的抗氧化作用,可作为潜在的天然抗氧化剂来源.

  2. Optimized prescription of Ganoderma Lucidum Polysaccharide Extract Dropping Pill%鹿角灵芝中灵芝多糖提取物滴丸成型工艺的优化

    Institute of Scientific and Technical Information of China (English)

    李星; 袁曦

    2015-01-01

    OBJECTIVE To study the feasibility of optimization the prescription of Ganoderma Lucidum Poly-saccharide Extract Dropping Pill.METHODS The central composite design and response surface method were used to optimize prescription of Ganoderma Lucidum Polysaccharide Extract Dropping Pill with PEG 6000 and Poloxamer 188 as combined carrier.And Ganoderma Lucidum Polysaccharide Extract Dropping Pill was prepared through melt-ing method.Independent variables were Ganoderma Lucidum Polysaccharide Extract content and Poloxamer 188 con-tent,while dependent variables were weight variable of pills and the dissolution rate of 5,10,60min.Quadratic poly-nomial fit-ting is adopted to estimate the mathematical relationship between independent and dependent varia-bles.Response surfaces were delineated according to the established mathematic models and optimum prescriptions were predicted from there.And confirmatory tests were carried out in the end.RESULTS The best prescription of Ganoderma Lucidum Polysaccharide Extract Dropping Pill was that Ganoderma Lucidum Polysaccharide Extract con-tent was 22%,while Poloxamer 188 content was 40%.Bias between observed and predicted values of all inspection indexes were negligible,indicating the high predictability of the fit models.CONCLUSION The central composite design and r-esponse surface method can be fairly used for optimizating the prescription of Ganoderma Lucidum Poly-saccharide Extract Dropping Pill,and provide new way for further exploration.%目的:探讨鹿角灵芝中灵芝多糖提取物滴丸处方条件,优化最佳处方。方法运用星点设计-效应面法优选鹿角灵芝中灵芝多糖提取物滴丸处方,以PEG6000和Poloxamer 188为联合载体,采用熔融法制备滴丸,并以主药的质量分数及 Poloxamer 188的质量分数作为考察因素,以丸重差异以及5、10、60min溶出度为评价指标,采用二次多项式拟合建立考察因素与评价指标之间的数学关系,根据所建

  3. 正交试验优化芒果叶多糖的提取工艺%Extraction of Polysaccharides from MangiferaIndicaLinn Leaves by Orthogonal Test

    Institute of Scientific and Technical Information of China (English)

    林燕如; 陈宜菲; 陈樱

    2014-01-01

    The optimized Ultrasonic-assisted extraction of polysaccharides in mangifera indica leaves was studied. According to orthogonal design, the optimum condition for the extraction of polysaccharides were as follows:1∶50 of solid-solvent ratio, 50℃of ultrasonic temperture, 400 W of ultrasonic power, 20 min of ul-trasonic time, 3.5 times of the volume of 95%alcohol precipitated, which resulted in the amount of 9.98 mg/g of mangifera indica leaves polysaccharides.%对利用超声波辅助法提取芒果叶多糖的最佳工艺进行研究,结果表明,芒果叶多糖的最佳提取条件为:料液比1∶50、超声波温度50℃、超声波功率400 W、超声波时间20 min、3.5倍体积的95%乙醇进行沉淀,以此条件提取的多糖含量为9.979 mg/g.

  4. Acute Toxicity Test of Polysaccharides Krestin from Coriolus versicolor Extract with Parameters of Hepatocyte Damages, SGPT and SGOT Enzyme in Mice

    OpenAIRE

    Andita Ayu Mandasari; Sri Puji Astuti Wahyuningsih; Win Darmanto

    2015-01-01

    Coriolus versicolor is a mushroom that has polysaccharopeptide krestin (PSK) and polysaccharopeptide (PSP). Many reports showed that polysaccharide krestin as a compound which could reduce mutagen induction, radiation, and development of cancer spontaneusly. However, all substances entering in body could change become toxic depending on dosage. Therefore, this research was aimed to know the effects of PSK on hepatocyte damages, SGPT and SGOT enzymes. Polysaccharide krestin was given by intrap...

  5. Optimization of Extraction Process of Polysaccharides of Cornus and Determination of the Contents of loganin%山茱萸多糖提取工艺优化及马钱苷含量测定

    Institute of Scientific and Technical Information of China (English)

    常玲彬

    2011-01-01

    The Extraction Process of Zhongshan Cornus polysaccharides in leach liquors was studied through enzyme hydrolysis,and the extract the active ingredient of loganin were analysis by HPLC.The results showed that the best process of cornel polysaccharide extraction was the ratio of solvent to material was 1∶5;the optimum extraction time was 6 h,extraction temperacture was 80 ℃,the optimum addition of pectin enzyme was 0.55 g/L.The average content of loganin in leach liquors was 0.512 g/L by HPLC method.%采用L(934)正交设计试验,对山茱萸浸提液中山茱萸多糖的酶水解法提取工艺进行了优化研究,并对浸提液的中有效成分马钱苷含量进行了HPLC法分析。结果表明,山茱萸多糖浸提的最佳工艺为:液料比1∶5,浸提时间4 h,浸提温度80℃,果胶酶添加量0.55 g/L。用HPLC法测定出的山茱萸浸提液中马钱苷平均含量为0.512 g/L。

  6. 超声辅助复合酶法提取桑黄多糖%Extraction technology of polysaccharides from phellin us linteus by ultrasonic-assisted complex enzymatic method

    Institute of Scientific and Technical Information of China (English)

    尹秀莲; 游庆红

    2011-01-01

    探索超声辅助复合酶法提取桑黄多糖的最佳工艺.以多糖提取收率为指标,对超声时间、复合酶用量、作用时间、酶解温度及pH进行单因素试验研究.结果表明:超声辅助复合酶法提取桑黄多糖的最佳条件为超声时间300 s、固定pH 4.0,应用2.0%的木瓜蛋白酶、果胶酶和纤维素酶50℃酶解90 min后,多糖得率可达1.46%.该提取工艺多糖提取收率高,可应用于实际生产.%Ultrasonic assisted complex enzymatic method was used to optimize the extraction conditions of polysaceharides from phellinus linleus . Using the extraction yield of total polysaccharides as target, the process was optimized by investigating the influence of ultrasonic time, complex enzyme dosage, enzymolysis time, enzymolysis temperature and pH value. Results; Results showed that the optimal extraction conditions for polysaccharides from phetlinus tin tens by ultrasonic-assisted complex enzymatic method were: ultrasonic time 300 s. 2.0% caroid, 2.0% pectinase plus 2. 0% cellulase, extraction temperature 50 ℃. Extraction time 90 min and pH 4. 0.and in this condition, the polysaccharide yield could reach 1. 46%.

  7. The Effects of Phellinus linteus Polysaccharide Extracts on Cholesterol Efflux in Oxidized Low-Density Lipoprotein-Loaded THP-1 Macrophages.

    Science.gov (United States)

    Li, Xiao-hui; Li, Yan; Cheng, Zhao-yun; Cai, Xi-guo; Wang, Hong-min

    2015-06-01

    The removal of excess cellular cholesterol is critical for maintaining cellular cholesterol homeostasis. Phellinus linteus polysaccharide extracts (PLPEs) is an immunomudulatory agent with a molecular weight of 153 kd. Here, we analyzed the effects of PLPEs on cholesterol efflux in oxidized low-density lipoprotein (ox-LDL)-loaded THP-1 (human acute monocytic leukemia cell line) macrophages. Various concentrations of PLPEs (5, 10, 20, and 100 μg/mL) were used to treat cells. Cholesterol efflux analysis was performed to analyze the cholesterol efflux ratio in PLPE-treated cells. Semiquantitative reverse transcription-polymerase chain reaction and Western blot analysis were conducted to assess the expression of target genes. Low dose of PLPEs (5-20 μg/mL) dose dependently enhanced cholesterol efflux to apolipoprotein A-I (ApoA-I), evidenced by promoting the expression of adenosine 5'-triphosphate (ATP)-binding cassette A1, ATP-binding cassette G1, and peroxisome proliferation-activated receptor γ, key regulators for cholesterol efflux. Moreover, GW9662, a potent antagonist of peroxisome proliferation-activated receptor γ, inhibited PLPE (20 μg/mL)-promoted cholesterol efflux to ApoA-I in a dose-dependent fashion. However, high dose of PLPEs (100 μg/mL) inhibited cholesterol efflux to ApoA-I from ox-LDL-loaded THP-1 macrophages, enhanced the production of superoxide anion, decreased mitochondrial membrane potential and ATP levels, and raised nicotinamide adenine dinucleotide/nicotinamide adenine dinucleotide phosphate oxidase subunits. Thus, these results indicate that low and high doses of PLPEs exhibit opposite effects on cholesterol efflux from ox-LDL-loaded THP-1 cells.

  8. Induction of G1-phase cell cycle arrest and apoptosis pathway in MDA-MB-231 human breast cancer cells by sulfated polysaccharide extracted from Laurencia papillosa.

    Science.gov (United States)

    Murad, Hossam; Hawat, Mohammad; Ekhtiar, Adnan; AlJapawe, Abdulmunim; Abbas, Assef; Darwish, Hussein; Sbenati, Oula; Ghannam, Ahmed

    2016-01-01

    Marine algae consumption is linked to law cancer incidences in countries that traditionally consume marine products. Hence, Phytochemicals are considered as potential chemo-preventive and chemotherapeutic agents against cancer. We investigated the effects of the algal sulfated polysaccharide extract (ASPE) from the red marine alga L. papillosa on MDA-MB-231 human breast cancer cell line. Flow cytometry analysis was performed to study the cell viability, cell cycle arrest and apoptosis. Changes in the expression of certain genes associated with cell cycle regulation was conducted by PCR real time analyses. Further investigations on apoptotic molecules was performed by ROS measurement and protein profiling. ASPE at low doses (10 µg/ml), inhibited cell proliferation, and arrested proliferating MDA-MB-231 cells at G1-phase. However, higher doses (50 µg/ml), triggered apoptosis in those cells. The low dose of ASPE also caused up-regulation of Cip1/p21 and Kip1/p27 and down-regulation of cyclins D1, D2, and E1 transcripts and their related cyclin dependent kinases: Cdk2, Cdk4, and Cdk6. The higher doses of ASPE initiated a dose-dependent apoptotic death in MDA-MB-231 by induction of Bax transcripts, inhibition of Bcl-2 and cleavage of Caspase-3 protein. Over-generation of reactive oxygen species (ROS) were also observed in MDA-MB-231 treated cells. These findings indicated that ASPE induces G1-phase arrest and apoptosis in MDA-MB-231 cells. ASPE may serve as a potential therapeutic agent for breast cancer.

  9. 山楂多糖的提取条件优化及对保加利亚乳杆菌的增殖影响%Optimization of hawthorn polysaccharide extraction condition and growth-promoting effect on Lactobacillus bulgaricus

    Institute of Scientific and Technical Information of China (English)

    戴远臣; 侯红漫; 张公亮

    2012-01-01

    The hawthorn polysaccharide was extraction with the method of hot bath, and was put into the growth test of Lactobacillus bulgaricus. And with the help of orthogonal experimental design, extraction condition was optimized. The results showed that with the optimized conditions, that solid-liquid ratio of 1:20, time of 6h, the temperature at 90℃, the polysaccharide yield was up to 3.92%. The Hawthorn polysaccharide had growth-promoting effect on Lactobacillus bulgaricus most when polysaccharide ratio of 2%, pH value 6.5, temperature of 42℃. By comparing the growth curves, it showed hawthorn poiysaccharide had obvious positive effect on Lactobacillus bulgaricus.%采用热水浴法提取山楂多糖,正交设计优化提取条件,并将山楂多糖用于保加利亚乳杆菌的增殖试验.结果表明,山楂多糖的最佳提取条件为:料液比1∶20、提取时间6h、提取温度90℃.在此条件下,山楂多糖的提取率为3.92%.山楂多糖对保加利亚乳杆菌有促生作用并且在多糖添加量为2%,pH值为6.5,温度为42℃时促进效果最好.通过绘制生长曲线与普通培养基中生长曲线相比较,证明山楂多糖对保加利亚乳杆菌的生长有明显的促进作用.

  10. Extraction and Determination of Polysaccharide from Discarded Fi brous Roots of Radix Panacis Quingueforlii%西洋参参须废渣中参多糖的提取及含量测定

    Institute of Scientific and Technical Information of China (English)

    张仁权; 吕洁平; 程怡

    2001-01-01

    Objective:To establish a feasible method for extracti ng polysaccharide from the discarded fibrous roots of Radix Panacis Quingueforli i afterpanaquilon had been extracted, and determine the polysaccharide content. Methods:Enzymolysis technique and alcohol was applied for decolorization, and ph enol-sulfuric-acid method was used to determine the active polysaccharide conte nt.The content of trace element and heavy mental was measured by element-analyze r and atomic fluorescence photometer respectively. Results: The yield of polysac charide from the fibrous roots was close (about 11.7%) to the main root.And the content of heavy metal can match the national standard.Conclusion:I t is valuable to extract the polysaccharide from the discarded fibrous root of R adix Panacis Quingueforlii.%目的:从提取西洋参皂苷后的参须废渣中进行西洋参多糖 提取的可行性研究和测定。方法:采用酶解技术和乙醇等方法脱色处理参多糖,用酚-硫酸法测定活性多糖的含量;元素分析仪测无机元素的含量; 原子荧光光度计测重金属的含量。结果:参须中的西洋参多糖得率与主根中多糖的得率相近,以干燥品计算在 11.7% 左右,并含有多种微量元素;重金属分析符合国家卫生标准。结论:在西洋参参须废渣中提取西洋参多糖具有较大 开发利用价值。

  11. Chemical composition and molecular structure of polysaccharide-protein biopolymer from Durio zibethinus seed: extraction and purification process

    Directory of Open Access Journals (Sweden)

    Amid Bahareh

    2012-10-01

    Full Text Available Abstract Background The biological functions of natural biopolymers from plant sources depend on their chemical composition and molecular structure. In addition, the extraction and further processing conditions significantly influence the chemical and molecular structure of the plant biopolymer. The main objective of the present study was to characterize the chemical and molecular structure of a natural biopolymer from Durio zibethinus seed. A size-exclusion chromatography coupled to multi angle laser light-scattering (SEC-MALS was applied to analyze the molecular weight (Mw, number average molecular weight (Mn, and polydispersity index (Mw/Mn. Results The most abundant monosaccharide in the carbohydrate composition of durian seed gum were galactose (48.6-59.9%, glucose (37.1-45.1%, arabinose (0.58-3.41%, and xylose (0.3-3.21%. The predominant fatty acid of the lipid fraction from the durian seed gum were palmitic acid (C16:0, palmitoleic acid (C16:1, stearic acid (C18:0, oleic acid (C18:1, linoleic acid (C18:2, and linolenic acid (C18:2. The most abundant amino acids of durian seed gum were: leucine (30.9-37.3%, lysine (6.04-8.36%, aspartic acid (6.10-7.19%, glycine (6.07-7.42%, alanine (5.24-6.14%, glutamic acid (5.57-7.09%, valine (4.5-5.50%, proline (3.87-4.81%, serine (4.39-5.18%, threonine (3.44-6.50%, isoleucine (3.30-4.07%, and phenylalanine (3.11-9.04%. Conclusion The presence of essential amino acids in the chemical structure of durian seed gum reinforces its nutritional value.

  12. 超声波辅助酶法提取红腰豆多糖工艺优化%Optimal extraction technology of polysaccharides from red kindey bean using ultrasonic assistant with enzyme

    Institute of Scientific and Technical Information of China (English)

    黎英; 陈雪梅; 严月萍; 赖丹妮; 陈小红; 石小琼

    2015-01-01

    Red kidney bean is a kind of valuable edible and medicinal soy food, which contains rich nutrients and a lot of effective ingredients. Polysaccharides are the main active component of red kidney beans, which have a variety of pharmacological effects such as anti-aging, anti-virus, anti-oxidant, antitumor and decreasing blood sugar and blood fat. In recent years, there have been substantial studies on the healthcare value, nutrition composition, product development of red kidney beans, but the extraction process of polysaccharides from red kidney beans and the analysis on its structure and biological activity have not been explored yet. The study respectively used enzyme’s characteristics of effectively destroying the cell wall and keeping the activity of polysaccharide, etc, and the ultrasonic’s characteristics of rapidness and energy saving by producing the effect of cavitation, shearing and intense mixing in liquid with the dispersion effect, to damage the cell tissue. By using the combined enzymatic and ultrasonic method assisted to extract polysaccharides out of red kidney beans, taking red kidney beans as raw material and polysaccharides yield as the index, and fixing the temperature at 50℃, the study firstly applied the L9(34) orthogonal experiments to screen out the best ratio of compound enzymes; and then on the basis of single factor experiment, the Plackett-Burman design was used to analyze solid-liquid ratio, amount of complex enzyme, pH value for enzyme hydrolysis, enzymolysis time, ultrasonic power and ultrasonic time, and these 6 factors were significant. Furthermore, the main factors affecting polysaccharides yield (pH value for enzyme hydrolysis, ultrasonic power, amount of composite enzyme and ultrasonic time) were investigated and optimized by response surface design using Box-Behnken center-united experiment software. Meanwhile, red kidney beans polysaccharide structure had been preliminarily analyzed by using ultraviolet spectrum and

  13. Optimization of Polysaccharide Extraction from Rumex Gmelini Turcz.Using Response Surface Methodology%响应面法分析优化毛脉酸模多糖提取工艺

    Institute of Scientific and Technical Information of China (English)

    周玉; 孙晓佳; 孙莹; 张维君; 王振月

    2013-01-01

    Objective:The experient used response surface methoddogy(RSM) to optimize the best Rumex gmelini polysaccharide extraction conditions.Methods:Box-Behnke statistical design technology was used to optimize the method of Rumex gmelini polysaccharide extraction.With the response surface optimization,the optimum conditions were:extraction time 2.9h;the grinding particle size was 120 mesh;the alcohol precipitation concentration was 70% and the decoction was carried out for three times,and its gotten rate was about 7.12%.Conclusion:By using the response surface method the parameters of optimized extraction of Rumex gmelini polysaccharide are accurate and reliable,and have a practical valoe.%目的:采用响应面法优选毛脉酸模多糖提取工艺.方法:采用Box-Behnken的中心组合实验设计原理优化毛脉酸模多糖提取方法.结果:得到最佳工艺条件是:提取时间2.9h,粉碎粒度为120目,醇沉浓度为70%,重复3次,得到多糖的得率最高为7.12%.结论:采用响应面法优化得到的提取毛脉酸模多糖参数准确可靠,具有实用价值.

  14. Navicula sp. Sulfated Polysaccharide Gels Induced by Fe(III): Rheology and Microstructure

    OpenAIRE

    2016-01-01

    A sulfated polysaccharide extracted from Navicula sp. presented a yield of 4.4 (% w/w dry biomass basis). Analysis of the polysaccharide using gas chromatography showed that this polysaccharide contained glucose (29%), galactose (21%), rhamnose (10%), xylose (5%) and mannose (4%). This polysaccharide presented an average molecular weight of 107 kDa. Scanning electron microscopy (SEM) micrographs showed that the lyophilized Navicula sp. polysaccharide is an amorphous solid with particles of ir...

  15. 无花果多糖提取工艺优化及其超声波改性%Extraction process optimization and ultrasonic modification of polysaccharide fromFicus caricaL

    Institute of Scientific and Technical Information of China (English)

    王振斌; 刘加友; 马海乐; 孙亚钊; 王林; 闫景坤; 郭强

    2014-01-01

    Figs have traditionally been used as medicinal remedies for cancer and popular fruit of excellent source of nutrients. Polysaccharide is an important functional compound of fig and exhibits high anti-oxidant capacity. In order to promote the industrial development of fig and find out the effect of fig polysaccharide molecule modification methods on its activity, the objectives of this paper were 1) determine optimal conditions for water extraction and alcohol precipitation method by use of response surface design method; and 2) the antioxidant activity and molecular characterization of the extracted fig polysaccharides after treated with ultrasound. The traditional extraction method with water extraction and alcohol precipitation for fig were used in this study, and the extraction technology was optimization by response surface design method. And the molecular structure of polysaccharide was modified by ultrasonic wave. The optimal extraction conditions were: extraction time 21 min, extraction temperature 90℃, and the ratio of water to raw material 49:1. Under these conditions, the polysaccharide extraction efficiency was 3.03% for the first extracting, and 3.86%, and 94.62% for the following two extractions, which were all polysaccharide that can be extracted. Then the fig polysaccharides were modified by ultrasound under the condition of 600 W of ultrasonic power for 90 min with 5:2 (s:s) of ultrasonic on/off time ratio. The Fourier transform infrared spectroscopy analysis showed the absorption peak of 3 133 and 3 017 cm-1 was enhanced after the fig polysaccharides treated by ultrasound, which meant that a large number of C-O-C and C-O-H bonds were interrupted. The number average molecular weight and the weight average molecular weight of the polysaccharides were also decreased from 536 800, 1 061 000 Da down to 46 410, 93 870 Da determined by size exclusion chromatography with multi-angle light scattering. The ultrasound modified fig polysaccharides were

  16. 气升式反应器超声破碎海带提取硫酸酯多糖%Extraction of Sulfate Radical Polysaccharide from Laminaria Japonica Enhanced by Ultrasonic Wave in an Air-lift Reactor

    Institute of Scientific and Technical Information of China (English)

    王谦; 黄猛; 赵兵; 王玉春; 欧阳藩; 伍志春

    2001-01-01

    在内径8 cm、有效容积为1 L的气升式循环超声破碎浸提装置中, 进行了超声波强化海带硫酸酯多糖浸提实验. 在pH 5.0、提取温度40oC、液固比45、提取时间25 min、通气量75 L/h 、超声功率120 W、超声作用时间百分比为100%的工艺条件下,硫酸酯多糖的提取率可达1.86%, 比传统水提法高,且极大地缩短了提取时间,比相同条件下不用超声时的提取率(1.11%)高得多. 此法所得多糖的SO42- 含量(26.5%)比水法浸提(20.8%)和相同条件下不用超声时(21.3%)都要高,显示出超声波在强化海带硫酸酯多糖浸提方面的良好应用前景.%The extraction of sulfate radical polysaccharide from Laminaria Japonica enhanced by ultrasonic wave in an air-lift reactor was reported. The optimal experimental conditions in this reactor (diameter 8 cm, working volume 1 liter) were pH 5.0, operation temperature 40oC, mass ratio of liquid to solid 45, extraction time 25 min, air flow rate 75 L/h, ultrasonic power 120 W, ultrasonic duty cycle 100%. Under the above conditions, the extraction ratio of sulfate radical polysaccharide reached 1.86%, higher than that by treatment for 3 h in water at 100oC and that under the same conditions without ultrasonic wave applied. The content of SO42- in sulfate radical polysaccharide of the former was 5.7% higher due to application of ultrasonic wave. The experimental results showed that this novel extraction process is prospective in the extraction of polysaccharide from seaweed.

  17. 雪莲薯多糖浸提工艺及其对羟自由基清除作用研究%Study on the extraction technology of polysaccharide from yacon and the scavenging effect on the hydroxyl free radical

    Institute of Scientific and Technical Information of China (English)

    吕明生; 王淑军; 徐兴权; 盘赛坤; 刘姝; 张兆兵

    2011-01-01

    采用热水浸提法提取雪莲薯多糖,对最佳提取工艺参数分别进行单因素和正交实验,结果表明,对浸提效果影响程度的顺序为:浸提时间>浸提次数>浸提温度>料液比.最佳提取工艺参数为:浸提时间2h、浸提次数4次、浸提温度80℃、料液比1:25.雪莲薯多糖对羟自由基具有清除作用,当多糖浓度达到16mg/mL时,多糖对羟自由基清除率超过了50%.%The optimum extraction process methods using hot water to extract polysaccharide from yacon were studied. Experiments of single factor and L9 (34) orthogonal test were investigated. The order of factors effecting the quantity of the polysaccharide was extraction time, extraction times, extraction temperature, and solid to liquid ratio. The optimum extraction condition was extracting 2h, extracting 4 times, extraction temperature 80℃, and the solid to liquid ratio of 1∶25. While the concentration of polysaccharide was 16mg/mL, the yacon polysaccharide could scavenge more than 50% of the hydroxyl free radical.

  18. The Impact on Crude Polysaccharide Extraction of Seed and Peels Dregs of Hippophae with Different Broken Method%破壁方法对沙棘籽及果渣粗多糖提取率的影响

    Institute of Scientific and Technical Information of China (English)

    陈丽娜; 吴琼; 邹险峰; 张莉宏

    2014-01-01

    With seed and peels dregs of hippophae as material, polysaccharide was dissolved fully by breaking the cytoderm. Four methods with enzymatic, cold solvent, microwave and ultrasonic were compared to the amount of assessment indicators for Polysaccharide dissolved. The results showed that the best method in breaking the cytoderm was microwave treatment and the best conditions was:power 600 W;5 min/time broken;two times;interval 3 min, Under this condition, crude polysaccharide extraction rate of seed and peels dregs of hippophae was 1.892%.%以沙棘榨汁提油后的籽及果渣为原料,经过破壁手段而使多糖充分溶出。本试验采用生物酶法、冻溶、微波及超声波四种方法进行对比破壁,以粗多糖提取率为考核指标,确定了最佳细胞破壁方法为微波破碎,并且确定微波破碎工艺条件为:功率600 W,破碎5 min/次,破碎次数为2次,间歇3 min,在此条件下沙棘籽及果皮渣中粗多糖提取率为1.892%。

  19. Microwave-assisted enzymatic hydrolysis of extruding bean dregs for extraction of polysaccharides%微波协同酶法提取大豆多糖工艺的研究

    Institute of Scientific and Technical Information of China (English)

    陈红; 刘秀奇; 崔海月; 樊红秀; 王大为

    2011-01-01

    以挤压豆渣为原料,采用微波协同酶法提取大豆多糖并对其工艺进行优化。通过单因素实验和正交实验,确定微波协同酶法提取的最佳工艺条件:微波功率600W,微波处理时间7min、液料比15:1、纤维素酶用量1.5%、酶解温度50℃、酶解时间40min、pH5.0,在此工艺条件下,多糖得率为15.85%。%The present study aimed at developing an optimal procedure for the extraction of polysaccharides from the extruding bean dregs hydrolyzed with a commercial cellulase preparation under microwave assistance.The optimal values of crucial technological parameters for improved polysaccharides yield were determined by one-factor-at-a-time and orthogonal array design methods as follows:microwave powder 600 W for 7 min treatment,water/material ratio 15:1(mL/g),enzyme dosage 1.5%,pH 5.0 and hydrolysis temperature 50 ℃ for a hydrolysis duration of 40 min.Under such conditions,a maximum polysaccharides yield of up to 15.85% was obtained.

  20. 复合酶提取灵芝多糖工艺及其抗氧化能力研究%Study on Enzymatic Extraction process of Polysaccharide from GANODERMA and Its Oxidation Resistance

    Institute of Scientific and Technical Information of China (English)

    张天笑; 刘红兵; 张文竹; 李春霞; 管华诗

    2011-01-01

    [ Objective ] To obtain the optimal enzymatic extraction process of polysaccharide from GANODERMA and discuss its oxidation resistance in vitro. [ Method ] Using extraction rate of polysaccharide as index, the proportion of three enzymes and enzymolysis conditions were optimized by orthogonal test based on single factor test. The in vitro oxidation resistance of polysaccharide was determined by DPPH free radical model. [ Result ] Complex enzyme extraction was superior to single enzyme extraction, and the optimal use levels of complex enzymes were: cellulose of 1.5 %, benase of 0.8% and bromelain of 3.5 % ( mass fraction, relative to concentration of zymolyte). The optimum enzymolysis conditions were as following: pH value of 5.5 ,temperature of 50 ℃ and enzymolysis duration of 100 min. In comparison with GANODERMA polysaccharide extracted by water, that extracted using complex enzyme was stronger in DPPH radical-scavenging ability (P < 0.01 ), and the DPPH radical-scavenging rate assumed a rise trend along with the increase of polysaccharide concentration in the range of 0.8 - 4. 8 mg/ml.[ Conclusion] The study provides scientific basis for the development of GANODERMA polysaccharide.%[目的]获得复合酶提取灵芝多糖的最佳工艺,探讨灵芝多糖的体外抗氧化能力.[方法]以多糖提取率为指标,在单因素试验的基础上,采用正交试验对复合酶用量配比、酶解条件进行优化;采用清除1,1-二苯基苦基苯肼(DPPH)自由基模型评价灵芝多糖的体外抗氧化能力.[结果]复合酶提取优于单酶提取,酶用量最佳配比为纤维素酶1.5%、木瓜蛋白酶0.8%、菠萝蛋白酶3.5%(质量分数,相对于底物浓度);最佳酶解条件为pH 5.5,温度50℃、酶解时间为100 min.复合酶提取的灵芝多糖具有良好的清除DPPH自由基作用,其清除能力优于水提灵芝多糖(P<0.01),且在浓度0.8~4.8 mg/ml范围内,其对DPPH自由基的清除率随浓度增大而增大.[结论

  1. Study on polysaccharide ultrasonic-assisted extraction process and antioxidation activity in Salicornia%海芦笋多糖超声波辅助提取工艺及抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    王超; 甄润英

    2012-01-01

    研究海芦笋多糖的超声波辅助提取工艺条件及体外抗氧化活性.通过单因素和正交试验确定超声波辅助提取工艺,利用羟自由基抑制试验判断海芦笋多糖的抗氧化功能.结果表明:海芦笋多糖的最优提取条件为料液(水)比1∶15(m∶V)、超声波处理时间15 min、浸提温度70℃、浸提时间2h,该工艺下粗多糖提取率为6.12%,与传统浸提相比,提取效率明显提高;海芦笋多糖对羟自由基·OH有明显的清除作用,且作用效果随着浓度的增加而增强,对羟自由基的半数抑制率(IC50)为0.578 mg/mL.%The ultrasonic assisted extraction process of Polysaccharide in Salicornia and its antioxidation function were studied. The single-factor and orthogonal experiments were applied in the extraction process. The antioxidant capacity of Polysaccharide in Salicornia was estimated with the test of hydroxyl radical inhibition. The Result showed: The optimal extraction parameters were as follow: ultrasonic treatment time 15 min, extraction temperature 70 ℃, and sol-id/liquid( water) ratio 1 : 15, extraction tmie 2 h. Under the optimized conditions, the extraction yield of polysaccharides was 6. 12%. Compared with the traditional hot water extraction, the extraction efficiency was improved obviously. The test of antioxidant capacity showed that radical · OH was eliminated significantly, and the role of these effect increased with the increase of polysacchandes concentration with IC50 concentration 0. 578 mg/mL.

  2. Design of wireless intelligent warehouse management system based on K60 and CC2530%基于K60与CC2530的无线智能仓储管理系统的研究

    Institute of Scientific and Technical Information of China (English)

    闫文娟; 王红磊

    2016-01-01

    为了提高仓储管理系统的布线灵活度,提高仓储管理效率,本文设计了一种以K60与CC2530为核心的高性能无线智能仓储管理系统,系统采用ZigBee无线组网,集成了仓储环境数据采集、分析、处理和门禁管理等功能。通过仓储环境的实际应用,系统在数据采集、网络传输和控制方面性能稳定,降低了25%的人力成本,提高了30%的效率,达到了预期效果。%In order to improve warehouse management system wiring flexibility and improve the efficiency of warehouse man-agement, wireless intelligent warehouse management system is designed based on K60 and CC2530 in this paper, the system uses ZigBee wireless networking and integrates the functions of data acquisition, analysis, processing and access control of warehouse environment. Through the practical application of the storage environment,the system possess high-performance characteristic in data acquisition, network transmission and control system performance. System is applied to reduce labor cost of 25%,improve the efficiency of 30%. The experiment results are good.

  3. Effect of Water-Extracting Temperature on the Extraction Rate, Chemical Composition and Immune Activity of Polysaccharides From Red Seaweed Gracilariales lemaneiformis%不同提取水温对龙须菜多糖的得率、组成和免疫活性的影响

    Institute of Scientific and Technical Information of China (English)

    朱地琴; 潘迎捷; 唐庆九; 张劲松; 赵勇

    2012-01-01

    系统研究了不同提取水温(30~100℃,温度梯度10℃,共8组)对龙须菜非胶体粗多糖的得率、多糖、蛋白、硫酸基、3,6内醚半乳糖、半乳糖含量的影响,并进一步比较了各组多糖的体外免疫活性和抗病毒能力。结果表明:不同提取水温对龙须菜非胶体粗多糖的得率影响显著(P〈0.01),50℃提取水温组的粗多糖得率较高,蛋白含量较低(P〈0.01),在30~50℃提取水温范围内,总糖含量显著上升;50℃水温组非胶体粗多糖的硫酸基含量显著高于40℃组,各组非胶体多糖的3,6内醚半乳糖含量在15%~20%之间,而半乳糖的含量则在30%~40%;各组非胶体多糖均表现出显著的体外免疫活性,其中50℃提取水温组的免疫活性显著高于其它各组,各组非胶体多糖均没有表现出抗疱疹病毒(HSV-Ⅰ和Ⅱ)活性。因此,综合多糖得率、生化组成及免疫活性,建议龙须菜免疫多糖的最佳提取水温为50℃。%Red seaweed Gracilariales lemaneiformis a commercially important seaweed with high nutritional and pharmic value. G. lemaneiforrn is widely cultured in the coastal area of Shangdong, Zhejiang, Fujian, Guangdong and Hainan province. Previous studies have shown that polysaecharides form G. lemaneiformis has many pharmic functions, such as antitumor, antioxidation, antivirus and antimutagenic. Then, exploration andultilization of polysaccharides form G. lemaneiform have attracted more and more attention for the reserch cylce, aquaculture and pharmacy industry. Although imcreasing temperature will facilitate the improvement of extraction rate of polysaccharide and agar production from G. lemaneiformis, high water-extracting temperature will destroy immune activity and antiviral activity of seaweed polysaccharides. Therefore, the identification of appropriate water-extracting temperature is very important for the extraction and ultilization of polysaccharides form G. lemaneiform. Up

  4. 桑黄液体发酵培养条件和多糖提取工艺的优化%Optimization of Liquid Fermentation Conditions of Phellinus linteus and Mycel ial Polysaccharide Extraction Process

    Institute of Scientific and Technical Information of China (English)

    杨成凤; 郑凌君; 廖尊胜; 刘晓艳; 吕旭聪; 林占熺; 黄一帆; 赵超; 刘斌

    2015-01-01

    The culture conditions of solid and liquid fermentation of Phellinus linteus and mycelial polysaccharide extraction process are optimized.The result shows that the optimal carbon sources for liquid fermentation medium are corn powder and soybean powder and the optimal nitrogen sources are yeast extract and soybean powder,the best ratio of carbon to nitrogen is 25∶1,temperature is 28 ℃, liquid medium volume is 80 mL,rotating speed is 1 10 r/min,and fermentation time is 9 days.While Pennisetum sinese as the main base material is cultivated by solid fermentation,P .linteus mycelium grows well.The optimal fermentation conditions for P .linteus mycelium fermented with P .sinese are as follows:extraction temperature of 90 ℃,solid-liquid ratio of 1∶40,extraction time of 1.5 h, with 3 times-volume ethanol alcohol polysaccharide,under the above conditions,the polysaccharide yield reaches 12.06%,which has more mycelium polysaccharides than other cultivation methods.%对桑黄固体和液体发酵培养条件及菌丝多糖的提取工艺进行优化。结果表明:桑黄液体发酵培养基的最佳碳源是玉米粉和黄豆粉,最佳氮源是酵母浸出汁和黄豆粉,最佳碳氮比25∶1,温度28℃,装液量80 mL,转速110 r/min,发酵培养时间9天。以巨菌草为主要基料进行固体发酵培养,桑黄菌丝长势良好。巨菌草发酵培养的桑黄菌丝多糖的最佳提取工艺为温度90℃,料液比1∶40,提取时间1.5 h,以3倍体积的乙醇醇沉多糖的条件下多糖得率最高,达到12.06%,高于其他栽培方式的桑黄菌丝体多糖含量。

  5. Extraction and in Vitro Antioxidant Activity of Jujube-wine Residue Polysaccharide%枣酒渣多糖的提取和体外抗氧化性研究

    Institute of Scientific and Technical Information of China (English)

    林静雅; 刘邻渭; 严陇兵; 原田

    2012-01-01

    Objective: Search for the extraction condition and determine antioxidation ability of jujube-wine residue polysaccharide (ZRP). Methods: The supersonic extraction condition of ZRP was optimized through single factor and central combination design experiments. The antioxidation ability of the polysaccharide was tested and compared with ascorbic acid, jujube polysaccharide(ZP) and jujube-wine polysaccharide(ZWP). Results; Using the lab ultrasonic cleaner with 40 KHZ, the optemized extraction condition was that extracting by water at 85 °C for 47 min with liquid solide ratio of 30: 1, then the yeild of ZRP was 12.52 mg/g. The ZRP IC50 of ·OH and DPPH· was 2.10 mg/mL and 1.83 mg/mL respectively, and the ZRP reducing power was stronger than ZP and ZWP but weeker than ascobic acid. Conclusions: ZRP had preferable antioxidation ability in vitro test and could be used as food antioxidant.%目的:探讨枣酒渣多糖(ZRP)的提取务件及其抗氧化活性.方法:采用单因素及中心组合试验,建立超声辅助提取ZRP的优化条件,并以红枣多糖(ZP)、枣酒多糖(ZWP)及抗坏血酸为对照,对ZRP清除·OH、DPPH·的能力及还原力进行研究.结果:在超声频率40 kHz条件下,ZRP的最佳热水浸提工艺为:提取时间47 min、提取温度85 ℃、液固比5∶1,在此条件下多糖的提取率为12.52 mg/g.ZRP清除·OH和DPPH·的IC50值分别为2.10 mg/mL和1.83 mg/mL,还原力强于ZP和ZWP,弱于抗坏血酸.结论:枣酒渣多糖具有较强的体外抗氧化能力,可作为食品抗氧化剂.

  6. 响应面法优化紫苏多糖的水提工艺%Optimization of Water Extraction Process of Polysaccharide of Perilla by Response Surface Method

    Institute of Scientific and Technical Information of China (English)

    陈建斌; 李冲伟

    2014-01-01

    采用微波法和响应面分析法(RSM)对中药紫苏籽中多糖的水提工艺进行了优化。紫苏籽经过粉碎和石油醚脱脂,在单因素实验基础上,选择微波提取功率及提取时间、液料比为自变量,用最陡爬坡路径逼近最大提取率区域后,利用响应面中心组合优化设计了紫苏多糖的最佳水提工艺。结果表明:紫苏多糖的最佳水提工艺为微波功率610 W,微波提取时间1.31 min、液料比28.5∶1,紫苏多糖的提取率为1.99%,并对响应面法优化的提取工艺进行了验证,相差率为0.3%。本结论为紫苏多糖的研究提供了一种方便快捷的提取方法。%In this study, the single factor experiments and the response surface analysis (RSM) were used to optimize the water extraction process of polysaccharide from basil seed, a kind of Chinese medicine. The basil seed was smashed and skimmed using aether petrolei, then the single factor experiments were performed using microwave extraction power and time, material/liquid ratio as independent variables. Finally, the optimal levels of each factor were determined after the peak response area was impended by the steepest climbing method and the central composite response test and were carried out at the apogee.The response surface method results showed that the best extraction process was as follows: microwave power at 610 W, microwave extraction time for 1.31 min, and material/liquid ratio at 28.5∶1,under these conditions, the Perilla polysaccharides extraction rate is 1.99%, and the response surface method to optimize the extraction process was verified with 0.3 % difference. This conclusion was provided a convenient and rapid method for extraction of the polysaccharide.

  7. 甘草多糖超声辅助提取工艺优化及分子表征%Ultrasonic Assisted Extraction Optimization of Glycyrrhiza Polysaccharide and Its Molecular Characterization

    Institute of Scientific and Technical Information of China (English)

    何培新; 吴双双; 林莉; 闵玉涛; 许春平

    2016-01-01

    In this study, the ultrasonic assisted extraction conditions of glycyrrhiza polysaccharides were opti-mized and its molecular weight and structure were investigated. The single-factor experiment was used to deter mine the optimum conditions:ultrasonic power 500 W, liquid/solid ratio 20∶1 (mL/g), ultrasonic time 60 min, temperature 60℃. The monosaccharide composition of glycyrrhiza polysaccharide was analyzed by GC/MS. The result showed that the artificial and wild glycyrrhiza monosaccharide components was primarily contained man-nose and glucose, also including arabinose, ribose, xylose, galactose, glucuronic acid and galacturonic acid, indicating that artificial and wild glycyrrhiza polysaccharides were acidic polysaccharides. Finally, the gly-cyrrhiza polysaccharides were purified and two fractions was obtained. The relative molecular weight of artificial glycyrrhiza polysaccharides were 212 ku and 25.1 ku, respectively, while relative molecular weight of wild gly-cyrrhiza polysaccharides were 34.1 ku and 0.1 ku, respectively. This study provides a theoretical basis for the bioactivity investigation and application of glycyrrhiza polysaccharide.%优化超声辅助提取甘草多糖的条件,并对甘草多糖的分子量和结构进行了研究。单因素实验确定最佳提取条件为:设定超声功率500 W时,液料比为20∶1(mL/g),超声时间60 min,温度60℃。应用GC/MS对甘草多糖进行单糖组分分析,得出人工与野生甘草多糖的单糖组分主要是甘露糖和葡萄糖,另外还含有阿拉伯糖、核糖、木糖、半乳糖、葡萄糖醛酸和半乳糖醛酸,说明人工与野生甘草多糖均为酸性多糖。最后利用Sepharose CL-6B层析柱对甘草多糖进行分离纯化,测得人工甘草两个多糖组分相对分子量分别为212 ku和25.1 ku,野生甘草多糖两个组分的相对分子量分别为34.1 ku和0.1 ku。本研究为甘草多糖的生物活性研究和应用提供了理论基础。

  8. Fucans, sulfated polysaccharides extracted from brown seaweeds, inhibit vascular smooth muscle cell proliferation. I. Comparison with heparin for antiproliferative activity, binding and internalization.

    Science.gov (United States)

    Logeart, D; Prigent-Richard, S; Jozefonvicz, J; Letourneur, D

    1997-12-01

    Smooth muscle cell (SMC) proliferation is inhibited both in vivo and in vitro by heparin. However, the precise mechanisms of action are still not understood. The analogy between two sulfated polysaccharides, heparin and fucan, has led us to compare in detail their effects on SMC growth. We have prepared and characterized a 19 kDa fucan fraction from brown seaweed, Ascophyllum nodosum. Fucan affects the growth of SMCs in a time- and dose-dependent, reversible and non-toxic fashion. As determined by cell counting, [3H]thymidine incorporation, and microcytofluorimetry analysis, heparin was less active than fucan in inhibiting SMC growth. Fucan and heparin act by preferential blocking of G0/G1, thus decreasing the G0/S transition. Binding experiments with [125I]fucan indicated saturable, unlabeled-fucan displaceable binding sites with an apparent Kd of 30 nM. Moreover, displacement experiments performed with various polysaccharides revealed that antiproliferative compounds interacted with these membrane sites, but non-antiproliferative polysaccharides (dextran, chondroitin sulfate) did not, providing evidence of a correlation between binding to SMCs and their antiproliferative activity. When cells were exposed at 37 degrees C to a fluorescent 5-([4,6-dichlorotriazin-2-yl]-amino)fluorescein (DTAF)-fucan, internalization occurred and punctate vesicles were observed which accumulated rapidly in the perinuclear region as previously reported for heparin. Nuclear preparations (membranes + contents) of cultured SMCs previously incubated with radiolabeled heparin or fucan indicated the presence of radioactivity, suggesting an antiproliferative action of both polysaccharides at the nuclear level. Collectively, these observations indicated that fucan and heparin share some similar mechanisms of action, such as SMC growth inhibition, binding, and internalization. In the accompanying paper (Logeart et al., Eur. J. Cell Biol. 74, 1997, this issue), we describe the effect of fucans

  9. Determination of polysaccharide in Lycium barbarum L. extract powder containing malt dextrin%含麦芽糊精枸杞提取物干粉中多糖含量测定方法

    Institute of Scientific and Technical Information of China (English)

    贾东升; 崔施展; 谢晓亮; 温春秀; 李荣乔; 刘铭

    2015-01-01

    目的:建立含麦芽糊精的枸杞提取物干粉中多糖含量的测定方法。方法采用糖化酶水解麦芽糊精,醇沉后苯酚硫酸法测定多糖含量,优化糖化酶水解麦芽糊精的工艺,比较酶解麦芽糊精后测定多糖含量、直接测定多糖含量与样品中多糖含量真实值之间的差异。并考察糖化酶对枸杞多糖的水解能力。结果糖化酶水解麦芽糊精的最佳工艺条件为:酶解温度60℃、酶与底物比例为1∶5,反应时间为30 min。与样品中枸杞多糖含量的真实值相比,直接测定值显著偏高,组间比较数据有显著统计学差异(P<0.01),酶解麦芽糊精后测定值无显著性差异(P>0.05);与直接测定值相比,酶解麦芽糊精后测定值显著降低(P<0.01),且糖化酶对枸杞多糖无水解能力。结论糖化酶水解法可消除麦芽糊精对枸杞提取物干粉中多糖测定的影响,且方法简单、有效。%Objective To establish a method for determination of polysaccharide in Lycium barbarum L . extract powder containing with malt dextrin. Method Maltodextrin was treated with glucoamylase hydrolysis and ethanol precipitation successively;then the phenol-sulfuric acid method was used to determine the content of polysaccharide. The glucoamylase hydrolysis technolgy of malt dextrin was optimized, and the variation of polysaccharide content before and after hydrolysis, the difference of polysaccharide contents between direct determination and truth were compared. Morever, the hydrolysis effect of glucoamylase on Lycium barbarum L. was investigated. Results The optimum hydrolysis condition was: temperature 60℃, the ratio of enzyme to substrate 1∶5, hydrolysis time 30 min. The polysaccharide content determined directly was significantly higher than the real value (P0.05). Glucoamylase showed no hydrolysis effects on Lycium barbarum L. polysaccharide. Conclusion Glucoamylase hydrolysis can deplete the

  10. Study on Extraction Process Conditions of Rice Bran Polysaccharide by Aqueous Two-phase System%双水相萃取米糠多糖工艺条件的探究

    Institute of Scientific and Technical Information of China (English)

    秦微微; 金婷; 宋学东; 丁振铎; 张衡

    2014-01-01

    旨在探究聚乙二醇/硫酸铵双水相体系萃取米糠中多糖成分的最佳条件。以双水相系统的相比、分配系数、收率等为参数,探究了PEG 的相对分子质量、PEG 的质量分数以及硫酸铵质量分数对米糠多糖在两相系统中的分配行为的影响。结果表明:萃取最佳条件为PEG分子量为6000,硫酸铵质量分数为14.8%,PEG 6000质量分数为14.3%,多糖得率为0.826388。%The aqueous two-phase system of polyethylene glycol (PEG)/ammonium sulfate for extrac-tion of polysaccharide in rice bran is studied.Use the parameters such as the volume ratio of two pha-ses,the distribution coefficient and the yield to explore the effects of the molecular weight of PEG, the mass fraction of ammonium sulfate and PEG on distribution behavior of polysaccharide of rice bran in two-phase system.It is demonstrated that the optimum conditions of extraction are as follows:the molecular weight of PEG is 6000,the mass fraction of ammonium sulfate is 14.8%,the mass fraction of PEG 6000 is 14.3%,and the yield of polysaccharide is 0.826388.

  11. 松籽壳多糖超声辅助溶剂法提取及抗氧化性研究%Study on ultrasonic-solvent assisted extraction and antioxidant activity of pine nut shell polysaccharide

    Institute of Scientific and Technical Information of China (English)

    李桂娟; 李冲; 姜雪; 龚伟; 张财华; 杨丽

    2012-01-01

    Study on ultrasonic-solvent assisted extraction technology of soluble polysaccharuie from pine nut shell. Through single factor test and orthogonal experiment L9 ( 34 ) optimize extraction process. The removal efficiency of pine nut shell polysaccharide on hydroxyl radicals (OH · ) and superoxide anion (O2- · ) detected was investigated through removal experiment. The result demonstrate that the ultrasonic-assisted extraction optimum technological conditions as follows: ultrasonic time 30. 0 min, extraction temperature 60 ℃, solid-liquid ratio 1 : 50(m : V) , and ultrasonic extraction times 1; the pine nut shell polysaccharides have a strong scavenging capacity effect on hydroxyl radicals(OH · ) ,but a general scavenging capacity effect on superoxide anion (O2- · ).%用超声波辅助溶剂法提取松籽壳中可溶性多糖,通过单因素和正交试验L9(34)优化提取工艺.并通过清除试验研究松籽壳多糖清除羟基自由基(·OH)和超氧阴离子自由基(O2-·)的效果.结果表明:超声波辅助提取松籽壳多糖的最佳工艺条件为超声时间30 min,提取温度60℃,料液比1∶50(m∶V),超声提取次数1次,该条件下多糖含量为44.26%;松籽壳多糖具有较强的清除(·OH)能力,对(O2-·)的清除作用一般.

  12. 酵母多糖的提取、组分分析及对小鼠免疫功能的影响%Extraction, Component Analysis of Yeast Polysaccharide and Its Influence on Immunologic Function in Mice

    Institute of Scientific and Technical Information of China (English)

    王元秀; 李峰

    2009-01-01

    Objective To study the effect of waste beer yeast polysaccharide on immunologic function in mice. Methods With waste beer yeast as the material, the yeast polysaccharide was extracted with citric acid. The poly-saccharide content was determined with phenol-sulfuric acid method and component analysis was performed with pa-per chromatography. The male mice at the age of 18 months were randomly divided into two groups. One group was lavaged with 1.0 mL of 1% polysaccharide for 30 days, the other group was treated with equivalent normal saline for 30 days. Results The corrected carbon clearance value, liver and spleen index and the activities of superoxide dismutase(SOD) and catalase(CAT) as well as the content of malondialdehyde(MDA) in tissue homogenate were significantly different between the two groups. Conclusion Yeast polysaccharide can promote the growth of immune organs, enhance macrophage phagocytosis, significantly increase the activities of SOD and CAT, and meanwhile re-duce the MDA content in liver and spleen in mice.%目的 研究啤酒废酵母多糖对小鼠免疫功能的影响.方法 以啤酒废酵母为材料,用柠檬酸法提取酵母多糖,苯酚-硫酸法测定多糖含量,纸层析鉴定多糖组分.随机将18月龄的雄性小鼠分为2组,一组每天定时灌胃1%多糖1.0 mL,一组灌胃等量生理盐水,连续30 d.结果 2组小鼠的碳颗粒校正廓清指数、肝脏和脾脏指数以及组织匀浆中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量有显著差异.结论 酵母多糖可促进小鼠免疫器官生长,增强小鼠巨噬细胞的吞噬能力;可显著提高SOD、CAT活性,减少肝脏、脾脏中MDA的含量.

  13. Final report on the safety assessment of AloeAndongensis Extract, Aloe Andongensis Leaf Juice,aloe Arborescens Leaf Extract, Aloe Arborescens Leaf Juice, Aloe Arborescens Leaf Protoplasts, Aloe Barbadensis Flower Extract, Aloe Barbadensis Leaf, Aloe Barbadensis Leaf Extract, Aloe Barbadensis Leaf Juice,aloe Barbadensis Leaf Polysaccharides, Aloe Barbadensis Leaf Water, Aloe Ferox Leaf Extract, Aloe Ferox Leaf Juice, and Aloe Ferox Leaf Juice Extract.

    Science.gov (United States)

    2007-01-01

    Plant materials derived from the Aloe plant are used as cosmetic ingredients, including Aloe Andongensis Extract, Aloe Andongensis Leaf Juice, Aloe Arborescens Leaf Extract, Aloe Arborescens Leaf Juice, Aloe Arborescens Leaf Protoplasts, Aloe Barbadensis Flower Extract, Aloe Barbadensis Leaf, Aloe Barbadensis Leaf Extract, Aloe Barbadensis Leaf Juice, Aloe Barbadensis Leaf Polysaccharides, Aloe Barbadensis Leaf Water, Aloe Ferox Leaf Extract, Aloe Ferox Leaf Juice, and Aloe Ferox Leaf Juice Extract. These ingredients function primarily as skin-conditioning agents and are included in cosmetics only at low concentrations. The Aloe leaf consists of the pericyclic cells, found just below the plant's skin, and the inner central area of the leaf, i.e., the gel, which is used for cosmetic products. The pericyclic cells produce a bitter, yellow latex containing a number of anthraquinones, phototoxic compounds that are also gastrointestinal irritants responsible for cathartic effects. The gel contains polysaccharides, which can be acetylated, partially acetylated, or not acetylated. An industry established limit for anthraquinones in aloe-derived material for nonmedicinal use is 50 ppm or lower. Aloe-derived ingredients are used in a wide variety of cosmetic product types at concentrations of raw material that are 0.1% or less, although can be as high as 20%. The concentration of Aloe in the raw material also may vary from 100% to a low of 0.0005%. Oral administration of various anthraquinone components results in a rise in their blood concentrations, wide systemic distribution, accumulation in the liver and kidneys, and excretion in urine and feces; polysaccharide components are distributed systemically and metabolized into smaller molecules. aloe-derived material has fungicidal, antimicrobial, and antiviral activities, and has been effective in wound healing and infection treatment in animals. Aloe barbadensis (also known as Aloe vera)-derived ingredients were not toxic

  14. Antibiofilm Activity of Actinobacillus pleuropneumoniae Serotype 5 Capsular Polysaccharide

    Science.gov (United States)

    Karwacki, Michael T.; Kadouri, Daniel E.; Bendaoud, Meriem; Izano, Era A.; Sampathkumar, Vandana; Inzana, Thomas J.; Kaplan, Jeffrey B.

    2013-01-01

    Cell-free extracts isolated from colony biofilms of Actinobacillus pleuropneumoniae serotype 5 were found to inhibit biofilm formation by Staphylococcus aureus, S. epidermidis and Aggregatibacter actinomycetemcomitans, but not by A. pleuropneumoniae serotype 5 itself, in a 96-well microtiter plate assay. Physical and chemical analyses indicated that the antibiofilm activity in the extract was due to high-molecular-weight polysaccharide. Extracts isolated from a mutant strain deficient in the production of serotype 5 capsular polysaccharide did not exhibit antibiofilm activity. A plasmid harboring the serotype 5 capsule genes restored the antibiofilm activity in the mutant extract. Purified serotype 5 capsular polysaccharide also exhibited antibiofilm activity against S. aureus. A. pleuropneumoniae wild-type extracts did not inhibit S. aureus growth, but did inhibit S. aureus intercellular adhesion and binding of S. aureus cells to stainless steel surfaces. Furthermore, polystyrene surfaces coated with A. pleuropneumoniae wild-type extracts, but not with capsule-mutant extracts, resisted S. aureus biofilm formation. Our findings suggest that the A. pleuropneumoniae serotype 5 capsule inhibits cell-to-cell and cell-to-surface interactions of other bacteria. A. pleuropneumoniae serotype 5 capsular polysaccharide is one of a growing number of bacterial polysaccharides that exhibit broad-spectrum, nonbiocidal antibiofilm activity. Future studies on these antibiofilm polysaccharides may uncover novel functions for bacterial polysaccharides in nature, and may lead to the development of new classes of antibiofilm agents for industrial and clinical applications. PMID:23691104

  15. Antibiofilm activity of Actinobacillus pleuropneumoniae serotype 5 capsular polysaccharide.

    Directory of Open Access Journals (Sweden)

    Michael T Karwacki

    Full Text Available Cell-free extracts isolated from colony biofilms of Actinobacillus pleuropneumoniae serotype 5 were found to inhibit biofilm formation by Staphylococcus aureus, S. epidermidis and Aggregatibacter actinomycetemcomitans, but not by A. pleuropneumoniae serotype 5 itself, in a 96-well microtiter plate assay. Physical and chemical analyses indicated that the antibiofilm activity in the extract was due to high-molecular-weight polysaccharide. Extracts isolated from a mutant strain deficient in the production of serotype 5 capsular polysaccharide did not exhibit antibiofilm activity. A plasmid harboring the serotype 5 capsule genes restored the antibiofilm activity in the mutant extract. Purified serotype 5 capsular polysaccharide also exhibited antibiofilm activity against S. aureus. A. pleuropneumoniae wild-type extracts did not inhibit S. aureus growth, but did inhibit S. aureus intercellular adhesion and binding of S. aureus cells to stainless steel surfaces. Furthermore, polystyrene surfaces coated with A. pleuropneumoniae wild-type extracts, but not with capsule-mutant extracts, resisted S. aureus biofilm formation. Our findings suggest that the A. pleuropneumoniae serotype 5 capsule inhibits cell-to-cell and cell-to-surface interactions of other bacteria. A. pleuropneumoniae serotype 5 capsular polysaccharide is one of a growing number of bacterial polysaccharides that exhibit broad-spectrum, nonbiocidal antibiofilm activity. Future studies on these antibiofilm polysaccharides may uncover novel functions for bacterial polysaccharides in nature, and may lead to the development of new classes of antibiofilm agents for industrial and clinical applications.

  16. In Vitro Antioxidant Properties of Flavonoids and Polysaccharides Extract from Tobacco (Nicotiana tabacum L. Leaves

    Directory of Open Access Journals (Sweden)

    Jing-Lu Wang

    2012-09-01

    Full Text Available In the present study, antioxidant properties of flavonoids and polysaccharides from tobacco (Nicotiana tabacum L. leaves were evaluated in several in vitro systems, e.g., scavenging activities on hydroxyl, superoxide anion, 1,1-diphenyl-2-picrylhydrazyl (DPPH and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS radicals, and reducing power. Flavonoids showed much better activity than polysaccharides in scavenging activities on free radicals. When compared to the positive control, ascorbic acid, both showed weaker antioxidant potential. However, flavonoids possessed comparable superoxide anion, DPPH and ABTS radical scavenging abilities to ascorbic acid at high concentration (600 μg/mL. Meanwhile, it was found that flavonoids had prominent effects on the reducing power, which was equivalent to ascorbic acid, and was significantly higher than polysaccharides. These results clearly indicate that flavonoids are effective in scavenging free radicals and have the potential to be powerful antioxidants. Thus, tobacco leaves could be considered as a potential source of natural antioxidants for food, pharmaceutical, cosmetics or nutraceutical industries.

  17. Extraction and anticoagulant activity of sulfated polysaccharides from Caulerpa cupressoides var. lycopodium (Vahl C. Agardh (Chlorophyceae - doi: 10.4025/actascibiolsci.v33i2.6243 Extraction and anticoagulant activity of sulfated polysaccharides from Caulerpa cupressoides var. lycopodium (Vahl C. Agardh (Chlorophyceae - doi: 10.4025/actascibiolsci.v33i2.6243

    Directory of Open Access Journals (Sweden)

    Norma Maria Barros Benevides

    2011-05-01

    Full Text Available The reportedly low standard quality of heparin (HEP for use in cardiac surgeries has led to concern in the Brazilian and international markets. Sulfated polysaccharides (SPs from seaweeds have been regarded as promising substitutes for HEP. The aim of this study was to sequentially extract total SPs (TSPs from Caulerpa cupressoides (Chlorophyceae with papain in 100 mM sodium acetate buffer (pH 5.0 containing 5 mM cysteine and 5 mM EDTA, followed by fractionation by ion-exchange chromatography (DEAE-cellulose, and then evaluate the anticoagulant potential of SP fractions by activated partial thromboplastin time (APTT using normal human plasma and compare it to standard HEP (193 IU mg-1. The obtained fractions were chemically characterized by chemical composition and agarose gel electrophoresis. The yield was 4.61%, and three fractions of SP (F I, F II and F III eluted with 0.50, 0.75 and 1.00 M of NaCl, respectively, were observed on chromatography profiles; however, differences in charge densities patterns and degree of resolution among them were revealed by electrophoresis. SPs were capable of modifying APTT only in fractions eluted with 0.75 M of NaCl, whose activities were 23.37 and 25.76 IU mg-1, respectively, and the charge density was prerequisite to activity. Therefore, C. cupressoides is a source of SPs possessing low anticoagulant potential compared to HEP.The reportedly low standard quality of heparin (HEP for use in cardiac surgeries has led to concern in the Brazilian and international markets. Sulfated polysaccharides (SPs from seaweeds have been regarded as promising substitutes for HEP. The aim of this study was to sequentially extract total SPs (TSPs from Caulerpa cupressoides (Chlorophyceae with papain in 100 mM sodium acetate buffer (pH 5.0 containing 5 mM cysteine and 5 mM EDTA, followed by fractionation by ion-exchange chromatography (DEAE-cellulose, and then evaluate the anticoagulant potential of SP fractions by activated

  18. Study on Extraction and Antioxidant Activity of Water-soluble Polysaccharides from Moringa Oleifera%辣木茎叶中水溶性多糖的提取及抗氧化活性的研究

    Institute of Scientific and Technical Information of China (English)

    梁鹏; 甄润英

    2013-01-01

    In order to explore the extraction conditions of water-soluble polysaccharide from the Moringa oleifera leaves and antioxidant activity of it,dry powder was used as raw materials and using water as extracting solvent to extract polysaccharide from Moringa oleifera. The extraction temperature, time and solid-liquid ratio were optimized by orthogonal experiments. Then,use methods of Salicylic acid and Pyrogallol to test antioxidant activity on polysaccharide from Moringa oleifera in different concentrations respectively. The results showed that the extraction rate was up to 5.66%,the optimal extraction conditions was extract 120 min ,using 80℃hot water at a solid-liquid ratio of 1∶20 (g/mL). The polysaccharides from Moringa oleifera leaf had a certain degree of antioxidant activities in vitro:scavenge OH·oand O2-in a dose-dependant manner with the IC50 of 7.252 8 mg/ml and 2.501 1 mg/ml respectively.%  主要探讨辣木茎叶中水溶性多糖的提取工艺条件以及抗氧化活性。以辣木茎叶干粉为原料,采用水为提取剂,通过单因素和正交试验对浸提温度、浸提时间及料液比进行研究;采用水杨酸法和邻苯三酚法分别测定辣木多糖对羟自由基以及超氧阴离子的清除率,以确定提取物的抗氧化活性。实验条件下辣木茎叶多糖最佳提取工艺条件为料液比1∶20(g/mL)、浸提温度80℃、浸提时间120 min,在此条件下,辣木粗多糖的提取率可达5.66%;多糖提取物对羟自由基及超氧阴离子均有清除作用,且随着提取物浓度的提高对二者的清除作用逐渐增强,存在剂量效应关系。清除作用的半数抑制率(IC50)分别是7.2528 mg/mL和2.5011 mg/mL。

  19. 离子液体双水相萃取山楂黄酮和多糖的研究%Study on the Extraction of Hawthorn Flavonoids and Polysaccharide in Ionic Liquid Aqueous Two-Phase System

    Institute of Scientific and Technical Information of China (English)

    巩育军; 牛盛童; 黄学锋; 王键

    2014-01-01

    Partition behaviors of hawthorn flavonoids and polysaccharide in ionic liquid aqueous two-phase system(ILATPS)were studied by spectrophotometry.Effects of concentration of ionic liquid and ammonium sulfate,dosage of hawthorn and ultrasonic time on the extraction rate of hawthorn flavonoids and polysaccharide were investigated.The optimal extraction conditions were as follows:concentration of ionic liquid [Bmim]BF4 was 0.26~0.30 g·mL-1,concentration of ammonium sulfate was 0.08~0.10 g·mL-1,dosage of hawthorn was 0.14~0.17 g,ultrasonic time was 15~20 min.Under these conditions,extraction rate of hawthorn fla-vonoids was 86.4%~96.0% in the up phase,and extraction rate of polysaccharide was 75.2%~76.0% in the down phase.%采用分光光度法研究了山楂黄酮和多糖在[Bmim]BF4/(NH4)2 SO4双水相体系的分配行为,探讨了离子液体浓度、(NH4)2 SO4浓度、山楂用量和超声时间等因素对山楂黄酮和多糖萃取率的影响。确定最佳萃取条件为:离子液体[Bmim]BF4浓度0.26~0.30 g·mL-1,(NH4)2 SO4浓度0.08~0.10 g·mL-1,山楂用量0.14~0.17 g,超声时间15~20 min,在此优化条件下,双水相上相中黄酮的萃取率为86.4%~96.0%、下相中多糖的萃取率为75.2%~76.0%。

  20. 微波辅助提取榛子叶多糖提取工艺研究%Study on the Technology of Microwave-assisted Extraction of Hazelnut Leaf Polysaccharide

    Institute of Scientific and Technical Information of China (English)

    申希峰; 林松; 张莲姬

    2016-01-01

    With petroleum ether and 95% ethanol treated hazelnut leaf powder as raw material, microwave-assisted extraction of hazelnut leaf polysaccharide was studied. The impact of leaching temperature, leaching time, the solid-liquid ratio and microwave processing time on polysaccharide yield of hazelnut leaf were investigated. As the result, the optimum conditions obtained by L9(34 ) orthogonal experiment were as follows:leaching temperature was 60 ℃, leaching time was 2 h, solid-liquid ratio was 1:25 ( w:V) , microwave processing time was 3 min. Under the above conditions, the polysaccharide yield of hazelnut leaf was 2. 67%.%以经石油醚和95%乙醇处理过的榛子叶粉为原料,主要研究微波辅助提取榛子叶多糖的工艺条件。分别考察了浸提温度、浸提时间、料液比、微波处理时间对榛子叶多糖得率的影响,并采用L9(34)正交试验优化提取工艺,最后得出的最佳提取工艺条为:浸提温度为60℃,浸提时间为2 h,料液比为1:25( w:V),微波处理时间为3 min,此条件下榛子叶多糖得率为2.67%。

  1. Analysis of composition and antioxidant activities of polysaccharide extracted from Moso Bamboo-Leaf%竹叶多糖的组分及抗氧化活性分析

    Institute of Scientific and Technical Information of China (English)

    殷军; 葛青; 毛建卫; 方晟

    2013-01-01

    竹叶粉碎后经过高温水提醇沉得竹叶多糖粗品.用DEAE琼脂糖凝胶FF和丙烯葡聚糖凝胶S-100分离纯化出两种均一多糖BLP30-1、BLP30-2,并对这二种多糖进行组分分析.气相分析结果表明,两者都由岩藻糖、木糖、甘露糖、葡萄糖、半乳糖五种糖组成.采用尺寸排除色谱和激光光散射联用仪(SEC-LLS)测定分子量分别为2.9×1 04、2.1×l04u.对两种多糖进行抗氧化活性研究表明,其对DPPH自由基、超氧阴离子自由基、羟基自由基均有较高的抑制活性.%Crude polysaccharide was extracted by hot water and precipitated by ethanol. Two purified polysaccharides were obtained by DEAE Sepharose FF and Sephcryl S-100,components of BLP30-1 and BLP30-2 were studied by GC method. The result showed that they were all composed of fucose,xylose, mannose,glucose,galactose. The molecular weight of the polysaccharides was obtained by SEC-LLS and their molecular weight were 2.9x104u and 2.1 x104u,respectively. Available data obtained with in vitro models suggested that they showed significant inhibitory effects on DPPH radical,hydroxyl radical,superoxide radical.

  2. Extraction and Composition Analysis of the Polysaccharide in Rabdosia rubescens%冬凌草多糖的提取、分离及理化性质研究

    Institute of Scientific and Technical Information of China (English)

    侯飞; 刘克为; 梁惠; 高华

    2011-01-01

    为研究冬凌草多糖的理化性质和质量,对冬凌草采用热水、稀碱提取,乙醇沉淀,等电点法脱蛋白后得到冬凌草粗多糖,经Q Sepharose 4 Fast Flow离子交换层析柱和Sephacryl S-100凝胶柱进一步纯化后得到4个纯度较高的组分STPS-Ⅰ、STPS-Ⅱ、JTPS-Ⅰ、JTPS-Ⅱ.对各组分的总糖含量、糖醛酸含量、硫酸根含量以及蛋白含量进行了测定,利用高效液相色谱法测定了单糖组成和分子量.结果 显示冬凌草多糖不同组分的分子量相近,化学组成及单糖组成明显不同.%To study the physical and chemical properties and quality of polysaccharides in Rabdosia rubescens. Crude polysaccharides of Rabdosia rubescens were obtained by extraction in hot water and dilute alkali successively, precipitation with ethanol and removal of protein by method of isoelectric point. STPS- Ⅰ, STPS- Ⅱ,JTPS- Ⅰ, JTPS-Ⅱ were isolated by a combination of ion exchange chromatography on Q Sepharose 4 Fast Flow and gel permeation over Sephacryl S-100 and with high purity. Their chemical characteristics including the content of total sugar, uronic acid, sulfate and protein as well as monosaccharide composition and molecular weight were investigated. The results showed that the molecular weight of different components of polysaccharides in Rabdosia rubescens was similar, while chemical composition and monosaccharide composition obvious different.

  3. Optimization of Extraction of Polysaccharides from Opuntia dillenii Haw. by Dual-enzymatic Method and Its Determination Method%仙人掌多糖的双酶法提取及含量测定的优化

    Institute of Scientific and Technical Information of China (English)

    赵龙岩; 袁清霞; 李子娇; 彭晓敏; 曾富华

    2011-01-01

    The conditions for the determination of polysaccharides from the wild Opuntia dillenii Haw. with phenol-sulfuric acid method were optimized, which were as follows: 2 ml of Opuntia dillenii Haw. samples, 0.5 ml of 4 % phenol and 5 ml of sulphuric acid, shaken up and placed stationarily for 5 min. The samples were measured by spectrophotometer at the wavelength of 482 nm. The calibration curve was linear in the concentration range of 7.5 -37.5 μg/ml. The extraction of polysaccharides from Opuntia dillenii Haw. was performed after sample was treated by 0.7 % pectinase and 0.3 % cellulase at 40 ℃ for 1.5 h. Under these conditions, the yield of polysaccharides was 20.08 %with purity of 83.02 %.%优化了苯酚-硫酸法测定仙人掌多糖含量的条件:取多糖样品2 ml,加4%苯酚0.5 ml,迅速加入浓硫酸5 ml,摇匀后静置5 min,于482 nm处测定吸光度.多糖在7.5~37.5μg/ml浓度范围内与吸光度的线性关系良好.采用果胶酶和纤维素酶双酶提取法从仙人掌中提取仙人掌多糖,最佳提取条件为:果胶酶浓度0.7%,纤维素酶浓度0.3%,40℃提取1.5h.仙人掌多糖产率为20.08%,纯度为83.02%.

  4. 全缘栝楼多糖及提取物不同极性部位抗氧化研究%Antioxidant Activity of Polysaccharides and Different Polarity Chemical Fractions of Extract from Trichosanthes ovigera Bl

    Institute of Scientific and Technical Information of China (English)

    潘乔丹; 唐海燕; 黄元河; 韦贤; 李巨宝; 李雄富

    2015-01-01

    研究全缘栝楼多糖和提取物不同极性部位体外抗氧化活性。采用清除羟自由基(OH·)、[2,2'-连氨-(3-乙基苯并噻唑林-6-磺酸)二氨盐]自由基(ABTS+·)、DPPH测定法以及Fe3+还原/抗氧化能力(FRAP)法,评价全缘栝楼多糖和提取物不同极性部位的抗氧化能力。全缘栝楼多糖和提取物不同极性部位清除DPPH·能力均强于抗坏血酸(IC50=1.22 mg/mL),且多糖(IC50=0.030 mg/mL)和乙酸乙酯层(IC50=0.082 mg/mL)比茶多酚强(IC50=0.118 mg/mL);多糖(IC50=0.031 mg/mL)清除OH·能力比茶多酚(IC50=0.032 mg/mL)和抗坏血酸(IC50=0.044 mg/mL)强,而石油醚层(IC50=0.033 mg/mL)和乙酸乙酯层(IC50=0.038 mg/mL)强于抗坏血酸;清除ABTS+·能力均强于茶多酚(IC50=0.416 mg/mL),且多糖(IC50=0.008 mg/mL)强于抗坏血酸(IC50=0.011 mg/mL);Fe3+还原/抗氧化能力均强于茶多酚(FRAP =1310.8μmol/g),但比抗坏血酸弱(FRAP=31469μmol/g)。全缘栝楼多糖和提取物不同极性部位有较强的抗氧化能力,为其生物活性的深入研究提供了参考依据。%To study the antioxidant activity of polysaccharides and different polarity chemical fractions of extract from Trichosanthes ovigera. The antioxidant activities of polysaccharides and different polarity chemical fractions of extract from Trichosanthes ovigera were evaluated by DPPH free radical scavening assay , and hydroxyl free radical (OH·) scavenging assay, and [2, 2'-Azino-bis (3-ethylbenzo thia zoline-6-sulfonic acid)]diammonium salt radical (ABTS+·)and ferric reducing antioxidant power (FRAP)assay. DPPH free radical scavening activity of polysaccharides and different polarity chemical fractions of extract from Trichosanthes ovigera were higher than VC (IC50 1.22 mg/mL) , and polysaccharides (IC50 0.030 mg/mL) and ethylacetate fraction(IC50 0.082 mg/mL) were higher than tea polyphenol(IC50 0.118 mg/mL). OH

  5. In vitro antioxidant activity of polysaccharide from Gardenia jasminoides ellis

    Science.gov (United States)

    Fan, Y.; Ge, Z.; Luo, A.

    2011-01-01

    A water-soluble polysaccharide, GP, was isolated from Gardenia jasminoides Ellis through hot water extraction followed by ethanol precipitation. The in vitro free radicals scavenging tests exhibited that GP has significant scavenging abilities especially for ABTS, DPPH, and hydroxyl radicals, which suggests that the polysaccharide GP is a novel antioxidant. ?? 2011 Academic Journals.

  6. Research on double enzymatic extraction and antioxidation properties of soybean polysaccharide%双酶法提取大豆多糖及其抗氧化性研究

    Institute of Scientific and Technical Information of China (English)

    宋慧; 苗敬芝; 董玉玮

    2011-01-01

    本文以大豆粕为原料,采用双酶法提取大豆多糖,探讨了酶种类、双酶组合、酶解时间、pH、固液比等对提取率的影响,通过正交试验优化工艺条件,并对其抗氧化活性进行研究.结果表明,大豆多糖提取的最佳工艺条件为pH为6.0,酶解时间为6h,固液比1∶20,加酶量为酸性蛋白酶10%+风味酶8%,大豆多糖提取率为9.28%.大豆多糖对·OH、·O2-自由基表现出较强的清除能力,其IC5o分别为0.083mg/mL和0.078mg/mL.说明从大豆粕中提取的大豆多糖具有良好的抗氧化活性,可作为功能性食品基料添加到乳制品、饮料、面包、饼干等食品中,既增加了产品的保健功能,同时又提高了大豆粕的附加值,为大豆粕的综合开发利用提供技术支撑.%In this paper, soybean meal was used as raw material. Soybean polysaccharide was extracted by double enzymes. Effects of enzyme type, double enzymes combination, hydrolysis time, pH, solid/liquid ratio on extraction rate were discussed. Orthogonal test was used to optimize extracting conditions and antioxidative activities tests. The results showed that the optimum extracting conditions were pH 6. 0, hydrolysis for 6 hours, solid/liquid ratio 1-20, acid protease 10% and flavor enzyme 8% , extraction rate of soybean polysaccharide was 9. 28% . Soybean polysaccharide scavenge ability of ? OH and ? O-2. IC50were 0. 083mg/mL and 0. 078mg/mL respectively. Soybean polysaccharide, extracted from soybean meal had good antioxidative activities. It may be used as a basic functional food material adding to dairy product, beverage, bread, biscuit to provide added value to these products. The experiment can be used for technological support on comprehensive development and utilization of soybean meal.

  7. Antiviral polysaccharides isolated from Hong Kong brown seaweed Hydroclathrus clathratus

    Institute of Scientific and Technical Information of China (English)

    WANG; Hui; OOI; Engchoon; Vincent; ANG; Put; O; Jr.

    2007-01-01

    Two relatively pure polysaccharides H3-a1 and H3-b1 had been isolated from the brown seaweed Hydroclathrus clathratus. They were characterized by HPLC, ultraviolet scanning, gas chromatography, infrared spectroscopy and elemental analysis, and shown to be two different sulfated polysaccharides with different monosaccharide content, but both with high relative molecular mass. They contained some proteins and uronic acid respectively. The sulfate content and bioactivity of these polysaccharides varied during purification. The fractions derived from the hot water extract also exhibited low anticoagulant effect. This is the first time that the antiherpetic and anticoagulant activities were evaluated for the polysaccharides from the Hong Kong brown seaweed Hydroclathrus clathratus.

  8. Optimization of extraction technique of polysaccharides from truffles via response surface methodology(RSM)%响应面法优化块菌多糖提取工艺条件的研究

    Institute of Scientific and Technical Information of China (English)

    张世奇; 阚建全

    2011-01-01

    Optimal processing parameters for ultrasonic-assisted extraction of truffle polysaccharide in neutral water medium were determined.On the basis of single factor experiments and according to Box-Behnken test design principles,the method of response surface method(RSM)for optimal parameters was adopted.Finally,the optimal extraction process was obtained as follows:ultrasonic power 105W,ultrasonic processing time 40min,ratio of liquid to sample 25∶ 1,extraction temperature 75℃.Under these conditions,the yield of polysaccharide truffles reached 3.48%.The model turned out to be with good fitting degree and minor error.%为确定块菌多糖超声中性水提取的最佳工艺条件,以块菌多糖提取率为考察指标,在单因素实验的基础上,采用响应面法对主要提取工艺参数进行了优化,并得到了回归模型。研究结果表明:块菌多糖超声中性水提取的最优工艺条件参数为超声功率为105W,提取时间为40min,料液比为1∶25,提取温度为75℃。在此条件下,块菌多糖的提取率达到3.48%。实验证明模型拟合程度良好,误差较小,模型的选择合适。

  9. 复合酶法提取鸡腿菇多糖的工艺优化%Optimization of Extraction of Polysaccharides from Coprinus comatus by Composite Enzyme method

    Institute of Scientific and Technical Information of China (English)

    郑义; 李超; 王卫东; 王乃馨; 王健龙; 张雷; 尹蓬

    2011-01-01

    The optimum processing conditions of the enzymatic extraction of polysaccharides from Coprinus comatus were studied.By using papain and cellulase complex processing method,the effects of liquid-to-solid ratio,the amount of composite enzyme adding,mass ratio of papain to cellulase,enzymolysis time,enzymolysis pH value,extraction time on the yield of polysaccharides were investigated by the single factor experiments.Technology parameters were optimized by Box–Behnken statistical design,then regression mathematical model was established,the model fitted significantly well and the optimum combination was obtained.When the extraction parameters were controlled at enzymolysis temperature 51.4 ℃,enzymolysis pH value 5.2 and mass ratio of papain to cellulase 0.86,the polysaccharides yield could be up to 6.42%.%为优化鸡腿菇多糖的提取工艺,采用木瓜蛋白酶与纤维素酶复合处理,通过单因素试验研究了液料比、复合酶添加量、木瓜蛋白酶与纤维素酶质量比、酶解温度、pH值和提取时间对鸡腿菇多糖得率的影响。在单因素试验的基础上,采用Box-Benhnken中心组合试验设计,建立了具有较好预测性能的鸡腿菇多糖提取条件的回归模型,获得了复合酶法提取鸡腿菇多糖的最佳工艺,即酶解温度51.4℃、酶解pH值5.2、木瓜蛋白酶与纤维素酶质量比0.86,在此条件下鸡腿菇多糖得率可达6.42%。

  10. A Study on Acute Toxicity of Crude Polysaccharide Extracted from Juglans Mandshurica Maxim to Mice%核桃楸皮粗多糖的急性毒性实验研究

    Institute of Scientific and Technical Information of China (English)

    王光; 吴宜艳; 雷涛

    2013-01-01

    Objective To estimate the acute toxicity of crude polysaccharide extracted from juglans mandshurica maxim to mice. Method Crude polysaccharide extracted from juglans mandshurica maxim (concentration of 5. 4 mg/mL) was orally administrated to the mice at a dose of 40 mL/kg, three times per day, and was injected intraperitoneally to the mice at a dose of 20 mL/kg, two times per day. After administrated for 1 days,continuous observated 7 days. Result In the course of the test, the activity and diet of mice were normal, and there were no death of the mice. Autopsy revealed nothing remarkable. The maximum dosage of oral administration and the maximum dosage of intraperitoneal injection were 6. 48mg/10g and 2. 16 mg/l0g respectively. Conclusion Toxic effect of the rude polysaccharide extracted from juglans mandshurica maxim on the mice in the two administration routes was low.%目的 观察核桃楸皮粗多糖对小鼠的急性毒性.方法 将5.4 mg/mL的核桃楸皮粗多糖溶液,分别按照40 mL/kg每天3次灌胃,20 mL/kg每天2次腹腔注射,给药ld后,连续观察7d.结果 实验期间,小鼠的活动与饮食情况未见异常,未出现死亡.尸检各脏器无异常.小鼠的核桃楸皮粗多糖每天最大给药量:腹腔注射2.16 mg/10 g,灌胃6.48 mg/10 g.结论 通过两种给药方法证明,核桃楸皮粗多糖对小鼠的毒性作用较低.

  11. Extraction and Component Analysis of Polysaccharide from Pleurotus ostrcatus%野生糙皮侧耳多糖的提取、分离纯化和组分分析

    Institute of Scientific and Technical Information of China (English)

    樊小英; 朱越雄; 孙海一; 曹广力

    2011-01-01

    以野生糙皮侧耳子实体为材料,用水浸法及乙醇沉淀法提取多糖;用过氧化氢、重蒸酚、不同含量的活性碳进行脱色;然后用氢氧化锌法、三氯醋酸法、sevag法、胰蛋白酶与sevag法相结合的方法进行脱蛋白;再通过透析和柱层析作进一步分离纯化;最后以纸层析法进行组成糖分析.结果表明:野生糙皮侧耳的水溶性多糖以1.5%活性碳脱色效果最佳,胰蛋白酶与sevag法相结合可获得较好的脱蛋白效果;DEAE-纤维素柱层析结果显示粗多糖含两种多糖组分;纸层析结果显示主要多糖组分的组成糖为:阿拉伯糖、鼠李糖、岩藻糖、半乳糖或葡萄糖.%Polysaccharide was extracted from the fruitbodies of wild Pleurotus ostrcatus by means of water - ex traction and ethanol -deposition, decolorized with peroxyl, phenol and different content of activated carbon, and protein was taken off with zinc hydroxide, trichloroacetic acid, Sevag method and trypsin and Sevag combined method. And then purified by dialysis and DEAE -cellulose chromatography. Finally polysaccharide analysis was done by paper chromatography. The optimum conditions were as follows: decolorization with 1.5 % activated car bon, protein - taking off by trypsin Sevag combined method. Two kinds of polysaccharide were purified by DEAE cellulose chromatography, and the paper chromatography showsed that the polysaccharide was composed of arabi nose, rhamnose, fucose, galactose or glucose.

  12. Study on extraction technology and antioxidative activity of polysaccharides and triterpenoids from peel of Poria cocos%茯苓皮多糖和三萜类物质提取及其抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    陈红梅

    2015-01-01

    采用超声辅助提取法分别从茯苓皮中提取多糖和三萜类物质。考察提取溶剂、超声功率、液料比、提取温度、提取时间等对茯苓皮多糖和三萜类物质的影响。结果表明,选用乙醇提取多糖,选用乙酸乙酯浸提三萜类物质,最佳提取条件为:超声功率450 W,液料比为45 mL/g,提取温度80℃,提取时间35 min。通过对DPPH自由基清除作用、还原力、羟基自由基清除作用的测定,评价茯苓皮中多糖和三萜类物质的抗氧化活性。结果表明,抗氧化能力与质量浓度存在量效关系。在质量浓度达到0.4 mg/mL时,茯苓皮多糖和三萜类物质提取液对DPPH和羟基自由基的清除能力均高于VC;茯苓皮三萜类物质提取液还原力与VC相当。茯苓皮提取物作为一种天然的抗氧化剂具有良好的应用前景。%Microwave assisted extracting conditions of polysaccharides and triterpenoids from peel of Poria cocos were studied.The in-fluence of extraction solvent , solvent-material ratio , ultrasonic power , temperature , extraction time was evaluated by single factor ex-periments.The results showed that the polysaccharides were obtained by ethanol , while the triterpenoids was obtained by ethyl acetate . The optimal conditions were as follows .Ultrasonic power 450 W;solvent-material ratio , 45 mL/g;temperature , 80℃;and extraction time, 35 min.The antioxidant activity was evaluated based on DPPH radical scavenging capacity , reducing power and hydroxyl free radical scavenging capacity .The ability of antioxidative activity was increased with the inerease concentrations of polysaccharides and triterpenoids.When extracted at the concentration of 0.4 mg/mL, polysaccharides and triterpenoids revealed relatively better DPPH radical scavenging capacity and hydroxyl free radical scavenging capacity than V C .Reducing power of riterpenoids was comparable to those of V C .Therefore , the peel of Poria cocos

  13. 山楂多糖的提取条件优化及对保加利亚乳杆菌的增殖影响%Optimization of extraction condition of hawthorn polysaccharide and its growth-promoting effect on Lactobacillus bulgaricus

    Institute of Scientific and Technical Information of China (English)

    戴远臣; 侯红漫; 张公亮

    2012-01-01

    采用热水浴法提取山楂多糖,正交设计优化提取条件,并将山楂多糖用于保加利亚乳杆菌的增殖试验.结果表明,山楂多糖的最佳提取条件为:料液比1∶20、提取时间6h、提取温度90℃.在此条件下,山楂多糖的提取率为3.92%.山楂多糖对保加利亚乳杆菌有促生作用并且在多糖添加量为2%,pH值为6.5,温度为42℃时促进效果最好.通过绘制生长曲线与普通培养基中生长曲线相比较,证明山楂多糖对保加利亚乳杆菌的生长有明显的促进作用.%The Hawthorn polysaccharide was extraction with the method of hot bath, and its effect on the growth of Lactobacillus bulgaricus was evaluated. The extraction conditions were firstly optimized by the orthogonal experiment design. The results showed that the optimal extraction conditions were as follows: solid-liquid ratio 1:20, extraction time 6h and extraction temperature 90℃. Under these conditions, the polysaccharide extraction ratio was up to 3.92%. The hawthorn polysaccharide had a great growth-promoting effect on L. Bulgaricus when polysaccharide 2%, pH value 6.5 and temperature 42℃. By comparing the growth curves, it showed that hawthorn polysaccharide had obvious positive effect on the growth of L. Bulgaricus.

  14. 响应面法优化枸杞多糖提取的研究%Study on Optimization of the Extraction Technology of Lycium Barbarum Polysaccharide by Response Surface Methodology

    Institute of Scientific and Technical Information of China (English)

    刘琳; 鄢瑞明; 曾凡骏

    2011-01-01

    In this study, the method of polysaccharide extraction was hot water extraction and discussing the crushing of raw materials, decolor, pre-soaked in the three factors of pre-experimental extraction to the effect of the follow-up. With anthrone - sulfuric acid method, the content of LBP was tested under the 620nm and the yield was calculated. Box - Behnken statistical design technology was used to optimize the method of LBP extraction. With the response surface optimization, the optimum conditions were: extraction temperature 87℃, liquid ratio 1:26, extraction time 7h. On the condition, the highest yield of LBP was 8.2%. The results were not only had the best extraction conditions for LBP, but also the results of response surface design experiment was much higher the traditional orthogonal in the degree of accuracy. The reference was provided for flarge-scale factory production in the future.%实验采用热水浸提法提取多糖,以蒽酮—硫酸法在620nm下检测枸杞多糖的含量,计算出得率.采用Box -Behnken统计设计技术优化枸杞多糖提取方法,得到的最佳工艺条件是:提取温度87℃,料液比1∶26,提取时间7h,一次提取枸杞多糖得率最高可达8.2%.不仅得出了枸杞多糖的最佳提取条件,而且采用响应面设计实验得到的结果相对传统的正交实验准确度更高,为以后大规模工厂化生产提供了参考.

  15. Sulfated polysaccharide extracted of the green algae Caulerpa racemosa increase the enzymatic activity and paw edema induced by sPLA2 from Crotalus durissus terrificus venom

    Directory of Open Access Journals (Sweden)

    Camila L. Pires

    2013-08-01

    Full Text Available Sulfated polysaccharides derived from seaweed have shown great potential for use in the development of new drugs. In this study, we observed that a low-molecular-weight sulfated polysaccharide from Caulerpa racemosa, termed CrSP, could interact with secretory phospholipase A2 (sPLA2 isolated from Crotalus durissus terrificus venom. When native sPLA2 (14 kDa was incubated with CrSP, they formed a molecular complex (sPLA2:CrSP with a molecular mass of 32 kDa, approximately. Size exclusion chromatography experiments suggested that CrSP formed a stable complex with sPLA2. We belived that sPLA2 and SPCr are involved an ionic interaction between negatively charged CrSP and the positively charged basic amino acid residues of sPLA2, because this interaction induced significant changes in sPLA2 enzymatic and pharmacological activities. CrSP caused a significant increase in sPLA2 enzymatic and bactericidal activity and increased its edematogenic effect. A pharmacological assay showed that the myotoxic activity of sPLA2:CrSP is unrelated to its enzymatic activity and that sPLA2:CrSP may have a practical application as a natural antibacterial agent for use in humans and commercially raised animals.

  16. Optimization of Extraction Technology of Polysaccharides from Opuntia at Room Temperature and Its Scavenging Activity on Hydroxyl Free Radial%室温下仙人掌多糖提取工艺及对羟自由基清除作用研究

    Institute of Scientific and Technical Information of China (English)

    黄芳; 李丽; 吴晓霞

    2012-01-01

    An orthogonal test was conducted in this study, with a view to optimizing the ultrasonic wave assisted extraction technology of polysaccharides from Opuntia at room temperature. The effects of material-water ratio, extrac- tion time and extraction frequence on the extraction efficiency of polysaccharides were studied. The results showed that the optimum extraction conditions were that the material-water ratio was 35:1 (mL/g), the extraction time was 2.5 min and the extraction frequence was twice. It was concluded that the optimal extraction technology was reasonable and suitable for the extraction of polysaccharides from Opuntia. The research on anti-oxidation properties showed that the extracted polysaccharides had a scavenging activity on hydroxyl free radical.%[目的]优选室温下超声辅助提取仙人掌多糖的提取工艺.[方法]采用正交试验设计,分别考察液固比、超声时间、提取次数对提取率的影响.[结果]最佳提取工艺为:液固比35:1mL/g,超声时间25min,提取次数2次.[结论]该工艺合理可行,可用于仙人掌多糖的提取.对多糖提取液的抗氧化研究表明,仙人掌多糖对羟自由基有一定的清除作用.

  17. Study on Extraction and Antioxidant Activity of Polysaccharides from Daylily Leaves%黄花菜叶多糖的提取及抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    周纪东; 李余动

    2015-01-01

    采用水提醇沉法、单因素正交试验研究黄花菜叶多糖(polysaccharides from Daylily leaves, PDL)的提取工艺,通过考察PDL对3种自由基的清除效果来研究其抗氧化活性。结果显示,PDL最优提取条件为提取温度90℃,提取时间3 h,液固比30:1(mL/g),提取3次。在此条件下,PDL提取率为9.13%,多糖含量为6.05%。PDL对O2-·和·OH的清除率分别为55.31%和19.45%,对脂质过氧化物(lipid peroxide, LPO)的抑制率为42.17%。综上表明,PDL对3种自由基的清除效果不显著,其抗氧化活性不强。%The extraction of polysaccharides from Daylily leaves (PDL) by water-extracting and alcohol-precipitating was optimized using one-factor-at-a-time and orthogonal array method. Meanwhile, the antioxidant activity of PDL by its scavenging effect on three free radicals was investigated. The results showed that the optimal conditions for PDL extraction was determined as three extraction cycles with distilled water at a solvent/solid ratio of 30:1 (mL/g) at 95℃ for 3 h. Under these conditions, the extraction yield of PDL was 9.13%, the content of PDL was 6.05%. The scavenging rate of PDL for O2-·, ·OH and lipid peroxide (LPO) was 55.31%, 19.45% and 42.17%, respectively. All main findings showed that PDL had little scavenging effect on three free radicals and weak antioxidant activity.

  18. Application of Nano-Scale Precipitate Engineering of TiN-NbC Composite in 32mm K60-E2 Grade Plate Rolling

    Science.gov (United States)

    Subramanian, S. V.; Xiaoping, Ma; Wenjin, Nie; Xiaobing, Zhang

    A cost effective alloy design based on niobium microalloying was developed by Shagang for the production of 32 mm gage K60-E2 steel plate for manufacturing LSAW pipes of 1422 mm diameter. Two metallurgical challenges are (i) to meet enhanced CTOD toughness and (ii) to ensure consistent DWTT performance in accordance with the specification laid out by Gazprom Vniigaz.

  19. Polysaccharide in Edible Fungus in Dandong: Extraction and Comparison%丹东地区几种食用菌中菌类多糖的提取与比较

    Institute of Scientific and Technical Information of China (English)

    韦月平; 王鹏

    2011-01-01

    利用水提醇沉法对丹东地区的香菇、滑菇、金针菇、黑木耳等食用菌的子实体进行多糖提取,并用苯酚一硫酸法进行多糖含量的测定,找出水提醇沉法的最佳提取工艺,为工业化大规模生产改进生产工艺、提高产量等提供理论及实验基础。正交实验结果表明,温度是影响粗多糖得率的重要因素,水提法的最佳工艺为:液料比80:1(mL:g),提取时间3h,温度100℃。在此条件下,测得香菇多糖含量最高为5.45%;金针菇多糖次之,含量为5.15%;滑菇多糖含量为4.90%;黑木耳多糖含量最低为3.98%:比较结果表明,丹东地区的滑菇和金针菇的多糖值远高于其他地区的同类产品。%Polysaccharide in fruiting bodies of shiitake mushroom, pholiota nameko, needle mushroom and black fungus in Dandong was extracted by water extraction and alcohol precipitation. Its content was determined with phenol -sulfuric acid method. The optimum extraction process was also investigated so as to provide theoretical and experimental basis for improving the production method, increasing the output in large scale industrialized production. The results of orthogonal experiment show that temperature greatly affects the yield of rough polysaccha- ride. The technological conditions of water extraction are as follows : the ratio of liquid and material is 80 : 1 ( mL: g) ; extraction time is 3 h; temperature is 100 ℃. Under such conditions, the content of polysaccharide extracted from shiitake mushroom is the highest, which is 5.45% ; that from needle mushroom is 5.15% ; that from pholiota nameko is 4.90% and that from black fungus is the lowest, which is 3.98%. Comparison result shows that the content of polysaccharide in pholiota nameko and needle mushroom in Dandong are remarkably higher than that of those in other areas.

  20. 杏鲍菇深加工残渣多糖酶法微波辅助提取工艺优化%Optimization of microwave-assisted enzyme extraction process of polysaccharide from Pleurotus eryngii stalk residue after deep treatment

    Institute of Scientific and Technical Information of China (English)

    柯乐芹; 张东旭; 肖建中

    2014-01-01

    为了研究杏鲍菇残渣中多糖的酶处理-微波辅助提取工艺及生物活性,该文以杏鲍菇深加工后的残渣为原料,在纤维素酶处理的基础上,微波辅助法提取杏鲍菇多糖;利用响应面试验设计对提取工艺条件进行优化,并与传统热水提取方法进行比较;对杏鲍菇多糖进行抗氧化和抑菌活性评价。结果表明,微波辅助提取杏鲍菇多糖的较佳条件为:水料比35∶1 mL/g,提取时间15 min,微波功率570 W,此条件下多糖的提取率为12.11%±1.02%,比热水提取高出41.21%,且提取时间缩短了105 min。杏鲍菇多糖对1,1-二苯基-2-三硝基苯肼自由基、羟基自由基和超氧阴离子自由基具有一定的清除作用,其半数抑制浓度(IC50)分别为22.9、19和21.1 mg/mL,对枯草芽孢杆菌、大肠杆菌和金黄色葡萄球菌具有较好的抑制作用,其最低抑制质量浓度分别为8、16和16 mg/mL,对黑曲霉和酿酒酵母没有明显的抑制作用。研究结果为进一步开发杏鲍菇多糖功能和利用杏鲍菇残渣提供一定的技术依据。%Pleurotus eryngii is a kind of valuable edible and medicinal fungus, which contains a lot of effective ingredients. Modern pharmacological researches show that, Pleurotus eryngii fruit body has the function of decreasing blood pressure and reducing blood lipid. Polysaccharides are the main active components of Pleurotus eryngii, which have the antioxidant, antibacterial, antiviral and antitumor effects. The polysaccharide extraction methods include hot-water extraction, ultrasound, microwave, high temperature and high-pressure extraction. At present, the extraction of Pleurotus eryngii polysaccharides is mainly performed by hot-water extraction, which has long extraction time, high energy consumption and low extraction yield. Pleurotus eryngii stalk residue is the byproducts of fruit body processing. It contains rich protein and polysaccharide, and has

  1. Pharmacological Action of Adenophora Polysaccharides

    Institute of Scientific and Technical Information of China (English)

    李泱; 李春红; 唐富天; 李新芳

    2004-01-01

    Adenophora polysaccharides (AP), is an active principle extracted from the root of Adenophorae Potaninii Korsh originated in Gansu Province and isolated with boiling water. AP is isolated and purified from the crude drug by DEAE-cellulose and Sephadex G-200 column, with a white powder and mean molecular weight of 8.3×104 , and [α]D20 of AP is + 68. AP is only composed of glucose judging from the analysis of it with patina chromatography (PC) and gas chromatography-mass spectrometer (GC-MS) methods.

  2. Important Determinants for Fucoidan Bioactivity: A Critical Review of Structure-Function Relations and Extraction Methods for Fucose-Containing Sulfated Polysaccharides from Brown Seaweeds

    DEFF Research Database (Denmark)

    Ale, Marcel Tutor; Mikkelsen, Jørn Dalgaard; Meyer, Anne S.

    2011-01-01

    Seaweeds—or marine macroalgae—notably brown seaweeds in the class Phaeophyceae, contain fucoidan. Fucoidan designates a group of certain fucose-containing sulfated polysaccharides (FCSPs) that have a backbone built of (1→3)-linked α-l-fucopyranosyl or of alternating (1→3)- and (1→4)-linked α......-l-fucopyranosyl residues, but also include sulfated galactofucans with backbones built of (1→6)-β-d-galacto- and/or (1→2)-β-d-mannopyranosyl units with fucose or fuco-oligosaccharide branching, and/or glucuronic acid, xylose or glucose substitutions. These FCSPs offer several potentially beneficial bioactive functions...... for humans. The bioactive properties may vary depending on the source of seaweed, the compositional and structural traits, the content (charge density), distribution, and bonding of the sulfate substitutions, and the purity of the FCSP product. The preservation of the structural integrity of the FCSP...

  3. Optimization of Extraction of Water-solubility Polysaccharide from Gentiana scabra Bunge by Response Surface Methodology%响应面法优化水溶性龙胆多糖的提取研究

    Institute of Scientific and Technical Information of China (English)

    王晨瑜; 刘鑫; 张晶; 王战勇

    2014-01-01

    采用响应面设计法(RSM)优化水溶性龙胆多糖的提取条件。在单因素试验基础上,结合响应面法优化确定提取水溶性龙胆多糖的最适工艺条件如下:提取温度100℃,料液比1∶26.56(g/mL),提取时间3 h,提取次数2次。此条件下,水溶性龙胆多糖的提取率预测值为13.51%,水溶性龙胆多糖的试验提取率为(13.36±0.25)%,预测值与验证值吻合较好。%Response surface methodology (RSM) was used to optimize the extraction conditions of water-solubility polysaccharides from the Gentiana scabra Bunge (GSP). Combining single factor test and response surface methodology, the optimum extraction conditions were chosen as follows:extraction temperature was 100℃, ratio of water to raw material was 26.56, extraction time was 3 h, and number of extraction was 2. Under the optimize conditions, the predicted value of yield of GSP was 13.51%, and the experimental yield of GSP was(13.36± 0.25)%, which showed the results were reasonable.

  4. Nutritional evaluation of Ulva fasciata and polysaccharide extraction from U. fasciata%裂片石莼营养价值的评价及其多糖制备的初步研究

    Institute of Scientific and Technical Information of China (English)

    叶方方; 吴后波; 向文洲; 吴华莲; 戴世鲲; 贾其坤; 陈灿坤

    2015-01-01

    Seaweeds, as an excellent raw material for renewable energy, feed, nutritional health food, cosmetics and pharmaceutical products, possess numerous advantages such as fast growth, high yield, rich in nutrition, and no acquisition of arable land. Ulva fasciata is very rich in Southern China, butfew studies onits nutritional value and polysaccharide extractionhave been reported in China. Taxonomic identification and nutritional value on theU. fas-ciata collected from Fujian Province, and a new approach toimprove the polysaccharide extraction by microwave heating were carried out in this study. The results showed that (i) U. fasciata consists of 34.8% the total sugars, 27.6%protein, 25.2%amino acids and 27.6%fatty acids;(ii) the content of essential amino acids and score of ratio coefficient of amino acids (SRC) in U. fasciata are 10.1%and 79.77 respectively;(iii)it was proved that the heavy metals contents in U. fasciata meet the requirement of the national standard of seaweed products. Meanwhile, compared with the traditional heating extraction process, the polysaccharide yield of U. fasciata was enhanced by 21.35%, and the energy consumption was lowered by 58.51% through the orthogonal experiment optimization of polysaccharide extractionusing microwave heating. All the above results revealed that U.fasciata as a macroalga with high nutritional value, is expected to become an excellent prospective raw material for food, nutraceutical, feed and other bioproducts.%对采自福建的野生裂片石莼(Ulva fasciata)进行了分类学鉴定和营养价值评估;采用微波加热新方法,进一步研究了裂片石莼多糖提取工艺。研究结果表明,裂片石莼的总糖、粗蛋白质、总氨基酸和总脂含量分别为34.38%、27.60%、25.20%、11.70%;其必需氨基酸含量为10.0%,氨基酸比值系数分(SRC)=79.77;裂片石莼的重金属元素含量达到国家海藻制品的限量标准。研究结果还表明,通过正交实验优化

  5. 大叶冬青苦丁茶多糖提取、纯化与抗氧化活性研究%Extraction,Purification and Antioxidant Activity in Vitro of Polysaccharides from Kudingcha Made from Ilex latifolia Thunb

    Institute of Scientific and Technical Information of China (English)

    赵天湖; 范嘉龙; 闫冬; 徐人杰; 郎昌野; 孙怡; 曾晓雄

    2011-01-01

    Kudingcha made from Ilex latifolia Thunb was defatted with 85% ethanol, and then extracted with hot water,decolored by S-8 macroporous resin, precipitated by ethanol, and dried to afford crude polysaccharide (ILPS). Four purified polysaccharides, ILPS-1, ILPS-2, ILPS-3 and ILPS-4, were gained from crude ILPS by DEAE-52 anion-exchange chromatography. Furthermore. the antioxidant activities in vitro of the crude ILPS and its purified fractions were evaluated by determinations of scavenging activities on 1. 1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, superoxide anion radicals (O2· - ), hydroxyl radicals (·OH) , ferric reducing-antioxidant power (FRAP) and chelating capacity to Fe2+. The results indicated that ILPS exhibited strong antioxidant activity, and the antioxidant activity was significantly correlated with its concentration.%大叶冬青苦丁茶经85%乙醇溶液脱脂、热水提取、S-8大孔树脂脱色、醇沉、干燥,得到大叶冬青苦丁茶粗多糖.粗ILPS经DEAE-52纤维素阴离子交换层析柱分离,得到4个多糖组分ILPS-1,ILPS-2,ILPS-3和ILPS-4.采用化学法分别测定了粗多糖及其纯化组分的体外清除自由基(DPPH·自由基,O2-·自由基,·OH自由基)能力、还原能力以及螯合金属离子能力.结果表明,大叶冬青苦丁茶多糖具有较强的体外抗氧化活性,并且抗氧化能力与多糖浓度之间存在良好的相关性.

  6. 柠檬酸提取海带渣中多糖及其抗氧化活性与结构的研究%The extraction of polysaccharide from Laminaria japonica residue by citric acid and study on antioxidant activities and structural characteristics

    Institute of Scientific and Technical Information of China (English)

    卢茳虹; 林宗毅; 崔春; 游丽君; 赵谋明

    2012-01-01

    以海带渣为原料,采用柠檬酸提取法提取海带渣中多糖,并与直接柠檬酸提取的海带多糖进行抗氧化活性与结构比较。研究表明,海带渣多糖的提取率为7.00%±0.03%,其抗氧化活性与直接酸提的海带多糖相当,具有较好的氧自由基清除能力(ORAC值为130.13μmol Trolox/g)、DPPH自由基清除能力(18.89μmol Trolox/g)、ABTS自由基清除能力(53.58μmol Trolox/g)和还原力(37.56μmol Trolox/g),其中ORAC值比抗氧剂BHT高2倍。所得海带渣多糖的主要组分分子量为310182u(2.73%)和30515u(97.27%)。红外光谱图分析表明海带渣多糖与直接酸提的海带多糖结构相似。%Laminaria japonica residue was used as the material to obtain polysaccharide by the citric acid extraction,and the antioxidant activities and structural characteristics of the polysaccharide had been compared with those of polysaccharide obtained by direct citric acid extraction. The results showed that the yield of the polysaccharide was 7.00% ± 0.03%, and the antioxidant activities were similar with those of polysaccharide obtained by direct citric acid extraction, with the values of oxygen radical absorbance capacity, DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power of 130.13μmol Trolox/g, 18.89μmol Trolox/g ,53.58μmol Trolox/g and 37.56μmol Trolox/g, respectively.Moreover,the value of ORAC was 2 times higher than that of BHT. Gel permeation chromatography analysis indicated that the polysaccharide comprised two components, the molecular weights were 310 ] 82 u ( 2.73 % ) and 305 ] 5 u (97.27 % ) separately.The infrared ( I R ) spectra analysis demonstrated that the polysaccharide had resemblant structure with that obtained by direct citric acid extraction.

  7. 羊肚菌胞内多糖的提取及单糖组成分析∗%Extraction and Monosaccharide Composition of Intracellular Polysaccharide of Morchella conica Fr

    Institute of Scientific and Technical Information of China (English)

    矫丽曼

    2016-01-01

    以人工培养的羊肚菌菌丝为材料,通过单因素和正交试验的方法,筛选羊肚菌胞内多糖( MIP)的最佳提取方法,并用硅胶G薄层层析和高效液相色谱法对MIP纯品的单糖组分进行分析。结果表明,羊肚菌多糖最佳的提取工艺为加水倍数25倍、提取温度90℃、浸提时间2h、用95%乙醇沉淀多糖,其多糖得率为7.694%。其MIP由D-甘露糖和D-半乳糖两种单糖组成,摩尔比为1︰1.059。%The mycelium of the planted Morchella conica Fr as materials, the best extraction method of intracellu-lar polysaccharide by single factor analysis and orthogonal experiment were studied. The composition sugars of MIP was also analyzed with Silica gel G thin-layer chromatography and HPLC. The results show that the optimal extrac-tion technology is : the water multiple with 25 , the extractive temperature with 90℃, the extractive time with 2 hours, the alcohol concentration with 95%. At this condition, the yield is 7. 694% , and the MIP is constituted by D-mannose and D-galactose, the mol proportion is 1︰1. 059.

  8. Anticoagulant activity of sulfated polysaccharides fractions from an aqueous extract obtained from the red seaweed Halymenia floresia (Clemente C. Agardh - doi: 10.4025/actascitechnol.v33i4.9143

    Directory of Open Access Journals (Sweden)

    José Ariévilo Gurgel Rodrigues

    2011-09-01

    Full Text Available Heparin (HEP is known due to their side effects and the red seaweed Halymenia floresia (Hf sulfated polysaccharides (SP are heparinoids. In this study we purified the Hf-SP obtained from an aqueous extract and evaluated their anticoagulant activities. Hf-SP1 (25°C, Hf-SP2 (80°C and Hf-SP3 (80°C were sequentially isolated. Hf-SP3 had the highest sulfate content (37.45%. Hf-SP3 was fractionated by ion exchange chromatography on a DEAE-cellulose column using a NaCl gradient. Fractions were lyophilized and submitted to 0.5% agarose gel electrophoresis. The anticoagulant activity was evaluated by the activated partial thromboplastin time using rabbits plasma and expressed in international units per mg of SP using standard HEP (193 IU mg-1. The chromatographic procedure separated into four different SP fractions (F I, F II, F III and F IV eluted at concentrations of 0.50, 0.75, 1.00 and 1.25 M of NaCl, respectively, reveling among them different marked on charge density, when compared by electrophoresis. F III had the highest anticoagulant activity (10.72 IU mg-1, suggesting that the sulfate is important in this process. In conclusion, our results suggest that sequential extractions of Hf-SP are an important biotechnological tool for identification of novel anticoagulants and studies of structural characterization are already in progress.

  9. Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from Halymenia sp. (Rhodophyceae - doi: 10.4025/actascibiolsci.v34i1.6961

    Directory of Open Access Journals (Sweden)

    Norma Maria Barros Benevides

    2011-11-01

    Full Text Available Sulfated polysaccharides (SPs have attracted growing interest for various biotechnological applications. We evaluated the efficiency of two methods of drying SPs (M I and II extracted from Halymenia sp Rhodophyceae in order to compare the yield, purification and anticoagulant activity. The total SPs (TSPs were first extracted with papain in 100 mM sodium acetate (pH 5.0 containing cysteine and EDTA (5 mM. The TSPs obtained were dried in an oven (M I or lyophilized (M II and then examined by ion exchange chromatography (DEAE-cellulose using the NaCl gradient technique. The fractions were analyzed by 0.5% agarose gel electrophoresis and the in vitro anticoagulant activity was evaluated by the activated partial thromboplastin time test using normal human plasma and compared to heparin (HEP (193.00 IU mg-1. There was a difference in TSP yield of 19.05% and similar chromatographic SP profiles. Electrophoresis revealed fractions with distinct resolutions. The fractions eluted with 0.75 M of salt (M I and II were the most active, measuring 27.40 and 72.66 IU mg-1, respectively, when compared to HEP. Therefore, obtaining SP with anticoagulant activity from Halymenia sp. is more efficient by freeze-drying. 

  10. Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

    Directory of Open Access Journals (Sweden)

    Eva Guillamón

    2012-12-01

    Full Text Available Polysaccharides from mushrooms have attracted a great deal of attention due to the many healthy benefits they have demonstrated, such as immunomodulation, anticancer activity, prevention and treatment of cardiovascular diseases, antiviral and antimicrobial effects, among others. Isolation and purification of polysaccharides commonly involve several steps, and different techniques are actually available in order to increase extraction yield and purity. Studies have demonstrated that the molecular structure and arrangement significantly influence the biological activity; therefore, there is a wide range of analytical techniques for the elucidation of chemical structures. Different polysaccharides have been isolated from mushrooms, most of them consisting of β-linked glucans, such as lentinan from Lentinus edodes, pleuran from Pleurotus species, schizophyllan from Schizophyllum commune, calocyban from Calocybe indica, or ganoderan and ganopoly from Ganoderma lucidum. This article reviews the main methods of polysaccharide isolation and structural characterization, as well as some of the most important polysaccharides isolated from mushrooms and the healthy benefits they provide.

  11. A simple technique for removing plant polysaccharide contaminants from DNA.

    Science.gov (United States)

    Do, N; Adams, R P

    1991-02-01

    A survey of the inhibitory effects of various plant polysaccharides on DNA restrictions (HindIII and EcoRI) revealed that neutral polysaccharides (arabino-galactan, dextran, gum guar, gum locust bean, beta-glucan, inulin, laminaran, mannan and starch) were not very inhibitory. In contrast, acidic polysaccharides (carrageenan, dextran sulfate, gum ghatti, gum karaya, pectin and xylan) were very inhibitory, even at low concentrations. The Elutip-d (RPC-5 type resin) was evaluated for removal of the inhibitory polysaccharides. Used alone or in combination with a phenol/chloroform wash, it proved effective in removing the polysaccharide so that HindIII digestion was possible, except in the cases of carrageenan and dextran sulfate. In addition, the genomic DNA extracts from live oak (Quercus virginiana) and magnolia (Magnolia grandiflora) were sufficiently purified so that the DNAs could be restricted with both EcoRI and HindIII.

  12. Study on Extraction of Opuntia dillenii Haw.Polysaccharides by Acid Method%酸法提取仙人掌多糖工艺

    Institute of Scientific and Technical Information of China (English)

    袁清霞; 赵龙岩; 李子娇; 程杰; 曾富华

    2012-01-01

    为探索适合产业化的仙人掌多糖(Opuntia dillenii Haw.polysaccharides,ODPs)提取工艺,采用单因素及正交试验优化酸法提取ODPs条件;优化的苯酚硫酸法测定ODPs得率及含量;并用酶法结合盐酸法除蛋白,经透析,洗涤得到精制的ODPs.得到酸法提取ODPs最佳条件为:加入浓度为0.10 mol/L的硫酸溶液,液料比为20:1(mL/g),70℃提取2次,每次2h.平均产率达39.92%,多糖纯度达87.11%,多糖中蛋白含量降低至1.06%.

  13. 仙人掌粗多糖提取条件的优化%Optimization of Conditions for Extraction of Polysaccharides from Opuntia Dillenii Haw.

    Institute of Scientific and Technical Information of China (English)

    兰琦杰; 曾富华; 张松莲

    2006-01-01

    比较了超声破碎时间、料液比、pH 值对提取率仙人掌粗多糖(Polysaccharides from Opuntia dillenii Haw.,OPS)及活性的影响.结果表明,各因子对OPS的影响程度依次为pH值、超声破碎时间、料水比,影响OPS活性的主要因子是pH值,在酸性或碱性环境下提取到的仙人掌粗多糖活性较低.据回归模型及其期望函数途径进行模拟选优,并进行验证,得到OPS提取率的优化条件为超声破碎时间38 min左右,料液比1:36(g*mL-1),pH值7.16左右.

  14. Immunomodulatory dietary polysaccharides: a systematic review of the literature

    Directory of Open Access Journals (Sweden)

    Nelson Erika D

    2010-11-01

    Full Text Available Abstract Background A large body of literature suggests that certain polysaccharides affect immune system function. Much of this literature, however, consists of in vitro studies or studies in which polysaccharides were injected. Their immunologic effects following oral administration is less clear. The purpose of this systematic review was to consolidate and evaluate the available data regarding the specific immunologic effects of dietary polysaccharides. Methods Studies were identified by conducting PubMed and Google Scholar electronic searches and through reviews of polysaccharide article bibliographies. Only articles published in English were included in this review. Two researchers reviewed data on study design, control, sample size, results, and nature of outcome measures. Subsequent searches were conducted to gather information about polysaccharide safety, structure and composition, and disposition. Results We found 62 publications reporting statistically significant effects of orally ingested glucans, pectins, heteroglycans, glucomannans, fucoidans, galactomannans, arabinogalactans and mixed polysaccharide products in rodents. Fifteen controlled human studies reported that oral glucans, arabinogalactans, heteroglycans, and fucoidans exerted significant effects. Although some studies investigated anti-inflammatory effects, most studies investigated the ability of oral polysaccharides to stimulate the immune system. These studies, as well as safety and toxicity studies, suggest that these polysaccharide products appear to be largely well-tolerated. Conclusions Taken as a whole, the oral polysaccharide literature is highly heterogenous and is not sufficient to support broad product structure/function generalizations. Numerous dietary polysaccharides, particularly glucans, appear to elicit diverse immunomodulatory effects in numerous animal tissues, including the blood, GI tract and spleen. Glucan extracts from the Trametes versicolor

  15. Structural characterization and bioactivities of sulfated polysaccharide from Monostroma oxyspermum.

    Science.gov (United States)

    Seedevi, Palaniappan; Moovendhan, Meivelu; Sudharsan, Sadhasivam; Vasanthkumar, Shanmugam; Srinivasan, Alagiri; Vairamani, Shanmugam; Shanmugam, Annaian

    2015-01-01

    Sulfated polysaccharide was isolated from Monostroma oxyspermum through hot water extraction, anion-exchange and gel permeation column chromatography. The sulfated polysaccharide contained 92% of carbohydrate, 0% of protein, 7.8% of uronic acid, 22% of ash and 33% of moisture respectively. The elemental composition was analyzed using CHNS/O analyzer. The molecular weight of sulfated polysaccharide determined through PAGE was found to be as 55 kDa. Monosaccharides analysis revealed that sulfated polysaccharide was composed of rhamnose, fructose, galactose, xylose, and glucose. The structural features of sulfated polysaccharide were analyzed by NMR spectroscopy. Further the sulfated polysaccharide showed total antioxidant and DPPH free radical scavenging activity were as 66.29% at 250 μg/ml and 66.83% at 160 μg/ml respectively. The sulfated polysaccharide also showed ABTS scavenging ability and reducing power were as 83.88% at 125 μg/ml and 15.81% at 400 μg/ml respectively. The anticoagulant activity was determined for human plasma with respect to Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) was 20.09 IU and 1.79 IU at 25 μg/ml respectively. These results indicated that the sulfated polysaccharide from M. oxyspermum had potent antioxidant and anticoagulant activities.

  16. Extraction, Isolation, Structural Characterization and Anti-Tumor Properties of an Apigalacturonan-Rich Polysaccharide from the Sea Grass Zostera caespitosa Miki

    Directory of Open Access Journals (Sweden)

    Youjing Lv

    2015-06-01

    Full Text Available An apigalacturonan (AGA-rich polysaccharide, ZCMP, was isolated from the sea grass Zostera caespitosa Miki. The depolymerized fragments derived from ZCMP were obtained by either acidic degradation or pectinase degradation, and their structures were characterized by electrospray ionization collision-induced-dissociation mass spectrometry (ESI-CID-MS2 and nuclear magnetic resonance (NMR spectroscopy. The average molecular weight of ZCMP was 77.2 kD and it consisted of galacturonic acid (GalA, apiosefuranose (Api, galactose (Gal, rhamnose (Rha, arabinose (Ara, xylose (Xyl, and mannose (Man, at a molar ratio of 51.4꞉15.5꞉6.0꞉11.8꞉4.2꞉4.4꞉4.2. There were two regions of AGA (70% and rhamnogalacturonan-I (RG-Ι, 30% in ZCMP. AGA was composed of an α-1,4-D-galactopyranosyluronan backbone mainly substituted at the O-3 position by single Api residues. RG-Ι possessed a backbone of repeating disaccharide units of →4GalAα1,2Rhaα1→, with a few α-L-arabinose and β-D-galactose residues as side chains. The anti-angiogenesis assay showed that ZCMP inhibited the migratory activity of human umbilical vein endothelial cell (HUVECs, with no influence on endothelial cells growth. ZCMP also promoted macrophage phagocytosis. These findings of the present study demonstrated the potential anti-tumor activity of ZCMP through anti-angiogenic and immunoregulatory pathways.

  17. Cytochemical Localization of Polysaccharides in Dendrobium officinale and the Involvement of DoCSLA6 in the Synthesis of Mannan Polysaccharides.

    Science.gov (United States)

    He, Chunmei; Wu, Kunlin; Zhang, Jianxia; Liu, Xuncheng; Zeng, Songjun; Yu, Zhenming; Zhang, Xinghua; Teixeira da Silva, Jaime A; Deng, Rufang; Tan, Jianwen; Luo, Jianping; Duan, Jun

    2017-01-01

    Dendrobium officinale is a precious traditional Chinese medicinal plant because of its abundant polysaccharides found in stems. We determined the composition of water-soluble polysaccharides and starch content in D. officinale stems. The extracted water-soluble polysaccharide content was as high as 35% (w/w). Analysis of the composition of monosaccharides showed that the water-soluble polysaccharides were dominated by mannose, to a lesser extent glucose, and a small amount of galactose, in a molar ratio of 223:48:1. Although starch was also found, its content was less than 10%. This result indicated that the major polysaccharides in D. officinale stems were non-starch polysaccharides, which might be mannan polysaccharides. The polysaccharides formed granules and were stored in plastids similar to starch grains, were localized in D. officinale stems by semi-thin and ultrathin sections. CELLULOSE SYNTHASE-LIKE A (CSLA) family members encode mannan synthases that catalyze the formation of mannan polysaccharides. To determine whether the CSLA gene from D. officinale was responsible for the synthesis of mannan polysaccharides, 35S:DoCSLA6 transgenic lines were generated and characterized. Our results suggest that the CSLA family genes from D. officinale play an important role in the biosynthesis of mannan polysaccharides.

  18. Determination of optimum process for microwave method in extraction of bergamot polysaccharide using statistical analytical method%统计学分析方法确定微波法提取佛手多糖的最佳工艺

    Institute of Scientific and Technical Information of China (English)

    李忻; 纪跃芝; 李纯净; 于倩

    2012-01-01

    目的 利用微波提取方法确定提取佛手多糖的最佳工艺条件.方法以佛手多糖含量为指标,提取次数、提取时间、提取功率、料液比为因素,通过单因素试验确定因素水平,利用统计学分析方法对提取佛手多糖的工艺进行优化研究.结果 微波法提取佛手多糖的最佳工艺设计是:提取次数2次,浸提时间8 min,微波功率550 W,料液比1∶20.结论此方法可以为工业大规模生产佛手多糖提供可靠依据.%Objective To determine the optimum process for the microwave method in the extraction of bergamot polysac -charide. Methods With the bergamot polysaccharide content as the indicator, and the extraction times, extraction time, extraction power and solid-liquid ratio as the factors, the single-factor test was used to determine the factor level, and the statistical analytical method was used to optimize the process for the microwave method in the extraction of bergamot polysaccharide. Results The optimum process design for the microwave method in the extraction of bergamot polysaccha ride was: extraction times was 2, extraction time was 8 minutes, microwave power was 550 W and solid-liquid ratio was 1:20. Conclusion This method can provide a reliable basis for the industrial scale production of bergamot polysaccharides.

  19. 大孔树脂对桑黄多糖提取液中色素的吸附与解吸特性研究%Adsorption and desorption characteristics of macroporous resins to pigments of Phellinus linteus polysaccharide extraction

    Institute of Scientific and Technical Information of China (English)

    秦俊哲; 程伟; 杜军国

    2013-01-01

    In order to select a kind of resin which is better on purification of polysaccharide extracted from the fruiting bodies of Phellinus Igniarius. Compared the effects of DA201 and other 5 kinds of macroporous resins in the proessing of decolorization and removal effects of liquid protein. On static adsorption and desorption experiments, select the DA201 resin for static adsorption kinetics and desorption characteristics, investigated the resin saturated adsorption, decolorization rate, protein removing rate and total sugar retention rate. The results show: DA201 resin showed a good effects of deproteinization and decolorization, and total sugar retention rate was high. For the specific polysaccharide extraction, flow rate at 1. 0 mL/min, DA201 resin column to make protein and pigment adsorption reached saturation, need continuous sampling 10 multiple column volume (BV) at least, distilled water and 50% ethanol elution desorption effects was suitable. DA201 resin was suitable for the decolorization of polysaccharide,In order to make full use of DA201 resin adsorption properties, the string column method can be considered in industrial production.%为了选择对桑黄多糖提取液纯化效果较好的树脂,比较了DA201等5种大孔吸附树脂对桑黄多糖提取液的脱色和除蛋白质效果.对5种树脂进行静态吸附与解吸试验,选择DA201树脂研究静态吸附动力学曲线及其解吸特性,考察树脂的饱和吸附量、脱色率、蛋白质脱除率及总糖的保留率.DA201树脂对桑黄多糖提取液中的蛋白质和色素的吸附较多,表明其脱蛋白和脱色效果较好,且多糖的回收率较高.对于特定的多糖提取液,流速1.0 mL/min,过DA201树脂柱要使蛋白质和色素达到吸附饱和,需要连续进样10多个柱床体积(BV),蒸馏水和50%乙醇洗脱解吸效果均较好.DA201树脂较适于桑黄多糖提取液的初步纯化,为了充分发挥DA201树脂的吸附性能,工业生产上可考虑采用串柱法.

  20. Research Progress of the Extraction Procedures and Bioactivities of Brown Seaweed Polysaccharide%褐藻多糖的分离提取及生理活性研究进展

    Institute of Scientific and Technical Information of China (English)

    吴泽; 魏琦峰; 邱庆庆; 任秀莲

    2015-01-01

    褐藻多糖是海藻胶、褐藻糖胶和褐藻淀粉的统称,主要存在于褐藻中。褐藻糖胶作为其主要的生理活性物质,主要由L-岩藻糖和硫酸酯基组成,具有抗氧化、抗凝血、防癌抗肿瘤、抗病毒和消炎等活性。综述了褐藻多糖的提取分离方法和褐藻糖胶的生理活性研究进展,以期为褐藻多糖的应用提供参考。%Brown seaweeds polysaccharide usually contains alginate, fucoidan, laminam and mainly exist in brown seaweeds.As the main physiological active substances, fucoidan composes of L-fucose and sulfate groups.Fucoidan has different beneficial biological activities including antioxidant, anticoagulant, anti-cancer, anti-tumor, anti-virus and anti-inflammatory, etc.This paper reviewed the latest research progress of the extraction procedures and bioactivities of fucoidan, which was expected to provided reference for fucoidan application.

  1. Chemical characteristics and anti-proliferation activities of Ganoderma tsugae polysaccharides.

    Science.gov (United States)

    Chien, Rao-Chi; Yen, Ming-Tsung; Tseng, Yu-Hsiu; Mau, Jeng-Leun

    2015-09-05

    Polysaccharides were extracted by hot-water and hot-alkali from four forms of Ganoderma tsugae including mature and baby Ling chih, mycelium and filtrate. Different profiles of proximate composition and monosaccharide constituents, and element contents were found in the extracted polysaccharides from different extractions and different forms. The molecular weight distributions of polysaccharides were 2.8×10(4)-6.5×10(5)Da and their infrared spectra were comparable. The hot-alkali extracted polysaccharides exhibited better anti-proliferation on IMR32 cells than the hot-water extracted polysaccharides, which were in turn more effective than the hot-water extracts. Besides, most hot-water extracts and both extracted polysaccharides exhibited an anti-proliferation effect on Hep G2 cells. However, the hot-water extracts showed less effective in anti-proliferation of IMR32 and Hep G2 cells. Based on the anti-tumor effects, both polysaccharides could be prepared for use in the formulation of nutraceuticals and functional foods.

  2. Structural studies of the O-specific polysaccharide(s) from the lipopolysaccharide of Azospirillum brasilense type strain Sp7.

    Science.gov (United States)

    Sigida, Elena N; Fedonenko, Yuliya P; Shashkov, Alexander S; Zdorovenko, Evelina L; Konnova, Svetlana A; Ignatov, Vladimir V; Knirel, Yuriy A

    2013-10-18

    Lipopolysaccharide was obtained by phenol-water extraction from dried bacterial cells of Azospirillum brasilense type strain Sp7. Mild acid hydrolysis of the lipopolysaccharide followed by GPC on Sephadex G-50 resulted in a polysaccharide mixture, which was studied by composition and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy. The following polysaccharide structures were established, where italics indicate a non-stoichiometric (∼40%) 2-O-methylation of l-rhamnose.

  3. Optimization of Ultrasound-assisted Extraction of Fungus Polysaccharides from Head Smut of Sorghum by Response Surface Analysis%响应面法优化高梁丝黑穗真菌多糖超声提取工艺

    Institute of Scientific and Technical Information of China (English)

    樊金娟; 杨振华; 徐秀德; 朱峰; 朱延姝; 阮燕晔

    2012-01-01

    To optimize the ultrasound-assisted extraction of polysaccharides from Head smut of sorghum, based on single factor studies, a multiple quadratic regression describing the relationship between the yield of polysaccharides and extraction param-eters such as extraction time, temperature and ratio of water to material was established using a 3-factor, 3-level Box-Behnken statistical design, and response surface and contour plots with the extraction rate of polysaccharides as the response were drawn based on the model. The optimum conditions for extracting polysaccharides from Head smut of sorghum were obtained as follows: ultrasound treatment time 30.2 min, temperature 94.25℃ and ratio of water to raw material 50 mL/g. Under such conditions, the relative error between the experimental and theoretical extraction rates of polysaccharides was 2.945%, indicating that the goodness of fit of the established regression model was quiet good.%为确定高梁丝黑穗真菌多糖超声波提取的最佳工艺条件,在单因素试验基础上选取试验因素与水平,利用中心组合(Box—Behnken)试验设计原理,采用3因素3水平响应面分析法,获得多元二次线性回归方程,以多糖提取率为响应值作响应面和等高线图。结果表明:高梁丝黑穗真菌多糖最佳超声波提取工艺条件为超声时间30.2min、提取温度94.25℃、液料比50(mL/g),在此工艺条件下,多糖提取率1.745%,与理论预测值1.798%的相对误差为2.945%,拟合度较好。

  4. A Study of Optimization of Extraction Conditions and Antioxidant Activity for Polysaccharide from Honeysuckle by Response Surface Methodology%响应曲面法优化金银花多糖提取条件及抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    向佳兰; 王茜; 谢阳; 张菊; 庞均; 张彦明; 蒙大双; 宋九华

    2015-01-01

    Response Surface Methodology (RSM)optimizes the best ultrasonic extraction condition of polysaccharides from honeysuckle and studies its antioxidant activity. Based on the central composite design of RSM, the extraction time, pH and the solid-liquid ratio are extracted, and the effects of the independent variables and their interaction on the extraction yield of polysaccharide are investigated. Respectively studied the scavenge ability of honeysuckle polysaccharide on ·DPPH free radicals and ·OH free radical. With the best extraction conditions of 40 min, 30:1 (mL/g) liquid-solid ratio and 4 PH value, the honeysuckle polysaccharide extraction yield was up to 1.92%. Honeysuckle polysaccharide had strong scavenge capacity to ·DPPH and ·OH, the EC50 are 0.016 mg/ml and 0.21 mg/ml respectively. The optimized process is stable and feasible, and the experimental results are accurate and reliable.%文章采用响应曲面法优化超声提取金银花多糖的条件,研究金银花多糖的抗氧化活性. 依据响应曲面法的中心组合设计,以提取时间、pH及液料比为自变量,研究各自变量及其交互作用对多糖提取率的影响. 考察金银花多糖对·DPPH自由基和·OH自由基的清除效果.金银花多糖的最佳提取条件:时间40min、液料比30:1(mL/g),pH值为4,此时金银花多糖最大提取率达1.92%.金银花多糖对·DPPH自由基和·OH自由均有较强的清除能力,其EC50分别为0.016mg/mL和0.21mg/mL.该优化条件稳定、可行,实验结果准确可靠;金银花多糖对·OH和·DPPH自由基均有较理想的清除能力.

  5. Structural characterization of polysaccharides from bamboo

    Science.gov (United States)

    Kamil, Ruzaimah Nik Mohamad; Yusuf, Nur'aini Raman; Yunus, Normawati M.; Yusup, Suzana

    2014-10-01

    The alkaline and water soluble polysaccharides were isolate by sequential extractions with distilled water, 60% ethanol containing 1%, 5% and 8% NaOH. The samples were prepared at 60 °C for 3 h from local bamboo. The functional group of the sample were examined using FTIR analysis. The most precipitate obtained is from using 60% ethanol containing 8% NaOH with yield of 2.6%. The former 3 residues isolated by sequential extractions with distilled water, 60% ethanol containing 1% and 5% NaOH are barely visible after filtering with cellulose filter paper. The FTIR result showed that the water-soluble polysaccharides consisted mainly of OH group, CH group, CO indicates the carbohydrate and sugar chain. The sample weight loss was slightly decreased with increasing of temperature.

  6. 白术多糖WAM-1结构的色谱分析和原子力显微镜观察%Chromatographic Analysis and Atomic Force Microscope Observation of Polysaccharide Extracted from Atractylodes macrocephala Koidz.

    Institute of Scientific and Technical Information of China (English)

    伍乐芹; 姜绍芬; 张静

    2012-01-01

    Polysaccharides extracted from the stem of Atractylodes macrocephala Koidz by hot water,were fractionated by DEAE-52 cellulose chromatography,and purified by Sephadex G-200 gel filtration chromatography to obtain a fraction, named WAM-1. HPLC and GC analysis showed that WAM-1 was a homogeneous and consisted of glucose and galactose in the molar ratio of 3. 01: 1. The molecular morphology of WAM-1 was observed under an atomic force microscope (AFM).The results showed that WAM-1 existed in different forms with different concentrations. The concentration of polysaccharide had effects on the conformation and form of chain interactions, which might be related to the interaction of intramolecular and intermolecular hydrogen bonds. At 10 μg/mL,the morphology of WAM-1 was observed clearly in rigid chains with many branches.%通过热水浸提法从草本植物白术根茎提取的水溶性粗多糖,经DEAE-52纤维素柱层析分离和Sephadex G-200凝胶过滤柱层析纯化,得到组分WAM-1.采用高效液相色谱(HPLC)检测WAM-1的纯度,气相色谱(GC)对其单糖组分进行分析,原子力显微镜(AFM)对其分子外貌进行观测.结果显示:WAM-1为均一多糖,由葡萄糖和半乳糖以3.01:1摩尔比构成;在不同浓度溶液条件下,WAM-1分子以不同形态存在,多糖溶液的浓度对WAM-1的分子链构象及链间相互作用形式产生影响,推测可能与WAM-1分子内、分子间的氢键缔合作用有关.多糖浓度为10μg/mL时,可清晰的观察到WAM-1是以刚性链状形态存在,且具有多分支结构.

  7. Optimization of Corn Silk Polysaccharide Extraction Technique and Study on its Antiaging Action%玉米须多糖的提取工艺优化及抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    田龙

    2011-01-01

    研究了玉米须多糖的提取工艺条件和抗氧化活性.通过单因素试验和正交试验确定了最佳工艺参数,即超声处理次数350 ~450次,超声波功率300 W,浸提时间在5~6h,乙醇体积分数约60%.并按200、400 mg/kg给老年大鼠连续灌胃30 d,测血清MDA含量,脑和肝组织脂褐质(Lf)含量,皮肤和肝组织羟脯氨酸(HYP)含量及血清SOD,CAT,GSH - Px活性.结果表明:两剂量组均能明显降低老年大鼠血清MDA含量及脑和肝组织Lf含量,明显提高血清SOD,CAT,GSH - Px活性及皮肤HYP含量.%In this paper, the optimized extraction of corn silk polysaccharide and antiaging action was studied, and the best technological parameter was determined through the single factor experiment and the orthogonal test, namely, supersonic processing number of times was 350 -450, ultrasonic wave power was 300W, the proposed soa- king time was 5 -6h, and ethyl alcohol density was approximately 60%. 200 and 400mg/kg were given to the old rats every day for thirty days. The content of malon -dialdehyde (MDA) in serum, the contents of lipofuscin( Lf) in brain and liver, the content of hydrooxyproline (Hyp) in skin and liver and the activity of superoxide dismutase (SOD) , catalase (CAT) and glutathione peroxidase (GSH -Px) in serum were examined. The results showed that polysaccharide could significantly decrease the content of MDA in serum and the Lf content in brain and liver, but obviously increase the activity of SOD, CAT, GSH - Px in serum and the content of Hyp in skin of the old rats.

  8. Preparation of polysaccharides from cyanobacteria Nostoc commune and their antioxidant activities.

    Science.gov (United States)

    Wang, Hong-Bin; Wu, Sheng-Jun; Liu, Dou

    2014-01-01

    In this study, water soluble polysaccharides were prepared from cyanobacteria Nostoc commune by water extraction. Factors affecting the polysaccharide yields were investigated, and the optimum extraction conditions were determined as follows: time, 4h; temperature, 90 °C; the ratio of liquid to solid, 60:1 (v/w); and extraction times, 4. The extract was filtered, concentrated to ∼10% (w/v), precipitated with 3 volumes of ethanol, freeze-dried, and ground to yield a water soluble power. The polysaccharide content of the product was 96.7%, and the yield was 9.18% (w/w). Fourier transform infrared spectra demonstrated that the product samples were mainly composed of polysaccharides. The polysaccharides showed high hydroxyl radical scavenging activity (92.71%) and reducing capacity (0.445) at the concentration of 10 mg/mL. Copyright © 2013 Elsevier Ltd. All rights reserved.

  9. Pharmacological Action of Adenophora Polysaccharides

    Institute of Scientific and Technical Information of China (English)

    李泱; 李春红; 唐富天; 李新芳

    2004-01-01

    @@ Adenophora polysaccharides (AP), is an active principle extracted from the root of Adenophorae Potaninii Korsh originated in Gansu Province and isolated with boiling water(1). AP is isolated and purified from the crude drug by DEAE-cellulose and Sephadex G-200 column, with a white powder and mean molecular weight of 8.3 × 104 , and [α]D20of AP is + 68(1). AP is only composed of glucose judging from the analysis of it with patina chromatography (PC) and gas chromatography-mass spectrometer (GC-MS) methods.The methylation analysis showed that AP is composed of (1→6) linked glucose residues. The measure of nuclear magnetic resonance imaging (NMR) 1H NMR and 14C NMR techniques further proved that AP is α(l→6) linked by Dglucose. The structure of AP is as follows: -[→6]α-D-Glu(1-)n→ (2).

  10. Marine Origin Polysaccharides in Drug Delivery Systems

    Directory of Open Access Journals (Sweden)

    Matias J. Cardoso

    2016-02-01

    Full Text Available Oceans are a vast source of natural substances. In them, we find various compounds with wide biotechnological and biomedical applicabilities. The exploitation of the sea as a renewable source of biocompounds can have a positive impact on the development of new systems and devices for biomedical applications. Marine polysaccharides are among the most abundant materials in the seas, which contributes to a decrease of the extraction costs, besides their solubility behavior in aqueous solvents and extraction media, and their interaction with other biocompounds. Polysaccharides such as alginate, carrageenan and fucoidan can be extracted from algae, whereas chitosan and hyaluronan can be obtained from animal sources. Most marine polysaccharides have important biological properties such as biocompatibility, biodegradability, and anti-inflammatory activity, as well as adhesive and antimicrobial actions. Moreover, they can be modified in order to allow processing them into various shapes and sizes and may exhibit response dependence to external stimuli, such as pH and temperature. Due to these properties, these biomaterials have been studied as raw material for the construction of carrier devices for drugs, including particles, capsules and hydrogels. The devices are designed to achieve a controlled release of therapeutic agents in an attempt to fight against serious diseases, and to be used in advanced therapies, such as gene delivery or regenerative medicine.

  11. 超声波辅助热水浸提香菇多糖响应面优化工艺及其抗氧化活性的研究%Optimization of Ultrasonic-assisted Extraction Technology of Lentinan Polysaccharides by Response Surface Methodology and its Antioxidant Activity

    Institute of Scientific and Technical Information of China (English)

    彭川丛; 孔静; 游丽君; 马方励

    2011-01-01

    采用响应曲面对超声波辅助热水浸提香菇多糖提取条件进行优化,优化后的工艺为:水料比37:1,超声功率550 W,超声时间7.3min,多糖得率实际测定值为20.58%,与模型预测值22.37%接近,表明用响应面方法来优化香菇多糖提取工艺是可行的.与传统的热水煎煮工艺相比,采用超声波辅助热水浸提的方法提取香菇多糖,可使多糖得率提高33.29%,能耗降低55.51%.测定香菇提取液对1,1-二苯基-2-苦味酰基自由基(DPPH·)的清除能力,结果表明,该提取液对DPPH·自由基有一定的清除能力,且呈剂量相关性.%The response surface methodology was used to optimize the ultrasonic-assisted boiling water extraction of lentinan polysaccharides. Design expert analysis indicated that the optimal processing conditions were: water to solid ratio 37∶1, ultrasonic power 550W and ultrasonic extraction time 7.3min, respectively. The extraction yield of lentinan polysaccharide is 20.58% which is close to the predicted value of 22.37%, indicated that using the response surface methodology to optimize the yield of lentinan polysaccharides is reasonable. The optimized extraction technology is better than the traditional hot-water boiling, which increases the yield of lentinan polysaccharides by 33.29% and also decreases the energy consumption by 55.51%. The results showed that the extracting liquid of the lentinus had the capacity to scavenge DPPH radical and scavenging activity was increased with the polysaccharide concentration.

  12. Comparison of antioxidant and antiproliferation activities of polysaccharides from eight species of medicinal mushrooms.

    Science.gov (United States)

    Chen, Peiying; Yong, Yangyang; Gu, Yifan; Wang, Zeliang; Zhang, Shizhu; Lu, Ling

    2015-01-01

    Polysaccharides from mushrooms including Pleurotus eryngii, P. ostreatus, P. nebrodensis, Lentinus edodes, Hypsizygus marmoreus, Flammulina velutipes, Ganoderma lucidum, and Hericium erinaceus were isolated by water extraction and alcohol precipitation. Our results suggest that all tested polysaccharides have the significant antioxidant capacities of scavenging free radicals (1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals). Among them, the H. erinaceus polysaccharide exhibits the highest 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity, whereas the L. edodes polysaccharide shows the strongest scavenging ability for hydroxyl radicals. Furthermore, using the MCF-7 breast cancer cell line and HeLa cells, all 8 selected polysaccharides are able to inhibit the proliferation of tumor cells, but the strength of inhibition varied depending on the mushroom species and the concentration used. Notably, G. lucidum polysaccharide shows the highest inhibition activity on MCF-7 cells. By comparison, H. erinaceus polysaccharide has the strongest inhibitory effect on HeLa cells. Moreover, high-performance liquid chromatography with a carbohydrate analysis column showed significant differences in polysaccharide components among these mushrooms. Thus our data suggest that the different species of mushrooms have the variable functions because of their own specific polysaccharide components. The 8 mushroom polysaccharides have the potential to be used as valuable functional food additives or sources of therapeutic agents for antioxidant and cancer treatments, especially polysaccharides from H. erinaceus, L. edodes, and G. lucidum.

  13. Pleurotus tuber-regium Polysaccharides Attenuate Hyperglycemia and Oxidative Stress in Experimental Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Hui-Yu Huang

    2012-01-01

    Full Text Available Pleurotus tuber-regium contains polysaccharides that are responsible for pharmacological actions, and medicinal effects of these polysaccharides have not yet been studied in diabetic rats. We examined the antidiabetic, antihyperlipidemic, and antioxidant properties of P. tuber-regium polysaccharides in experimental diabetic rats. Forty rats were equally assigned as diabetic high-fat (DHF diet and polysaccharides treated DHF groups (DHF+1P, DHF+2P, and DHF+3P, 20 mg/kg bodyweight/8-week. Diabetes was induced by chronic low-dose streptozotocin injections and a high-fat diet to mimic type 2 diabetes. Polysaccharides (1P, 2P, and 3P were extracted from three different strains of P. tuber-regium. Fasting blood glucose and glycosylated hemoglobin (HbA1c levels substantially decreased, while serum insulin levels were restored by polysaccharides treatment compared to DHF. Furthermore, plasma total cholesterol, triglycerides, and low-density lipoprotein levels were significantly (P<0.01 lower in polysaccharide groups. High-density lipoprotein levels were attenuated with polysaccharides against diabetes condition. Polysaccharides inhibited (P<0.01 the lipid peroxidation index (malondialdehyde, and restored superoxide dismutase and glutathione peroxidase activities in the liver of diabetic rats. The antihyperglycemic property of polysaccharides perhaps boosts the antioxidant system that attenuates oxidative stress. We emphasize that P. tuber-regium polysaccharides can be considered as an alternative medicine to treat hyperglycemia and oxidative stress in diabetic rats.

  14. Orthogonal Array Design for the Optimization of Successive Extraction of Total Flavonoids and Polysaccharides from Epimedium brevicornum Maxim%正交试验优化淫羊藿总黄酮和多糖的分步提取工艺优化

    Institute of Scientific and Technical Information of China (English)

    付亮; 袁璟亚; 杨瑞武; 张利; 周永红; 丁春邦

    2012-01-01

    Objective: To optimize process conditions for the extraction of total flavonoids from the whole plant of Epimedium brevicornum Maxim and for further extraction of polysaccharides from the remaining residue. Methods: A two- step sequential extraction procedure was proposed for the extraction of total flavonoids by ultrasonic-assisted extraction and polysaccharides by hot water extraction. Results: The optimum conditions for the extraction of total flavonoids were found to be two extraction cycles at 60 ℃ and a microwave power of 150 W for 5 min each cycle with 70% ethanol at a solid-to- solvent ratio of 1:10 (g/mL), resulting in an extraction efficiency of 4.72%. The optimum conditions for the extraction of polysaccharides were found to be two extraction cycles at 90 ℃ for 30 min each cycle with a residue-to-water ratio of 1:10 g/mL, resulting in an extraction efficiency of 1.89%. The optimized procedure for sequential extraction of total flavonoids and polysaccharides was characterized by easy operation, low energy consumption, high extraction efficiency, low polysaccharide loss and enhanced comprehensive utilization ofEpimedium brevicornum Maxim.%通过对淫羊藿总黄酮和多糖进行分步提取工艺优化,探寻一种提高淫羊藿综合利用率的提取工艺。利用单因素结合正交试验优化淫羊藿总黄酮的超声波辅助提取工艺,并对此工艺所得药渣进行多糖的水煮提取工艺优化。结果表明,总黄酮提取的最优工艺条件为按料液Lk1:10(斯nL)加入70%乙醇溶液,在60℃提取温度、150W超声功率下提取2次,每次5min,提取效率达4.72%;多糖提取的最优工艺条件为按料液比1:10加入水,在90℃提取温度提取2次,每次30min,提取效率达1.89%。优化后的淫羊藿总黄酮和多糖分步提取工艺操作简单、能耗低、提取效率高、多糖损失率低、较大限度地提高了淫羊藿的综合利用率。

  15. OPTIMIZATION OF MICROWAVE-ASSISTED EXTRACTION CONDITIONS OF MYCELIUM POLYSACCHARIDES FROM GRIFOLA FRONDOSA BY RESPONSE SURFACE METHODOLOGY%用响应面法优化灰树花菌丝体多糖微波提取工艺的研究

    Institute of Scientific and Technical Information of China (English)

    郭家瑞; 王卫国; 李鹏; 崔羽佳

    2011-01-01

    在单因素试验的基础上,利用响应面法分析优化了微波提取灰树花茵丝体多糖的工艺条件,并与直接水提法进行了比较.结果表明,微波辅助提取灰树花茵丝体多糖的最佳工艺条件为:微波功率570.21 W、提取时间6.91 min、液料比(mL/g )40.29∶1,提取两次,预测最大提取率为11.33%,验证试验的实际提取率为11.30%,与理论预测值的相对误差仅为0.26%,和直接水提法相比,多糖提取率提高了4倍.%On the basis of single factor experiments, we optimized the microwave-assisted extraction conditions of mycelium polysaccharides from Grifola frondosa by response surface methodology (RSM), and compared the results with that of the water extraction method. The results showed that the optimal extraction conditions of microwave-assisted extraction method were as follows: microwave power 570.21 W, extraction time 6.91 min and liquid-to-material ratio 40.29:1 (V:M), extraction twice; the actual extraction rate was 11.30%, which has a small relative error in comparison to the predictive value (11.33%); and the polysaccharide extraction rate of the microwave-assisted extraction method was larger than that of the water extraction method by four times.

  16. The First Report of Vibrio parahaemolyticus Strain O10:K60 in Japan, a New Combination of O and K Serotypes Isolated from a Patient with Gastroenteritis.

    Science.gov (United States)

    Ueno, Hiroyuki; Tomari, Kentaro; Kikuchi, Koji; Kobori, Sumie; Miyazaki, Motonobu

    2016-01-01

    Vibrio parahaemolyticus is an important pathogen that causes gastroenteritis in humans, generally associated with the consumption of contaminated seafood, particularly raw shellfish. There are many serotypes in V. parahaemolyticus resulting from a combination of O and K antigens. Among them, O3:K6 and their variants, which represent the pandemic clone, are the most widespread strains worldwide. In this study, we examined V. parahaemolyticus isolated from a gastroenteritis patient's stool at a hospital in Saitama City, Japan in 2013. Serotyping of the O and K antigens identified the strain as O10:K60. To our knowledge, this is the first reported case of a V. parahaemolyticus strain with this antigen combination in Japan. Subsequently, we used PCR to assay for pathogenicity-associated genes, and found that it was positive for tdh, T3SS1, and T3SS2α genes. Antibiotic susceptibility tests showed that the strain was susceptible to all selected antibiotics except ampicillin. Moreover, we detected specific marker genes for the pandemic clone with two kinds of PCR assay. Our results suggest that the isolate O10:K60 is a newly emerging serotype that belongs to the pandemic clone.

  17. Characterization of active polysaccharides of HemoHIM

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Kwang Sun; Shin, Myeong Suk; Bae, Beom Seon; Hwang, Yong Cheol [Kyonggi University, Suwon (Korea, Republic of); Ryu, Kwang Won [Chungju University, Chungju (Korea, Republic of)

    2007-07-15

    In this study, we aimed to elucidate the detailed structure and active moiety of polysaccharide, one of the active constituents of immune and hematopoietic modulating activities of HemoHIM. We first isolated the polysaccharide fractions from the hot water extracts of the each ingredient herbs (A. gigas, P. janonica, C. officinale) of HemoHIM and their mixture. These polysaccharides were composed of neutral (85.32-92.73%) and acidic (4.25-7.88%) saccharides, proteins (0.16-4.02%), and polyphenols (2.09-5.37%). The hydrolytic analysis of polysaccharide fractions showed that they commonly showed higher arabinose, galactose, and galacturonic acid contents. These result suggested that these polysaccharides may have higher contents of rhamnogalacturonan among pectic substances and the main active moiety is composed of polysaccharides. The anion exchange chromatography of HemoHIM and each ingredient herb extract using DEAE-Sepharose FF (Cl- form) column resulted in 1 non-adsorption and 8 adsorption fractions. The analysis of immune activity (lymphocyte proliferation) on these fractions showed that the fractions obtained by higher salt concentration carried the higher activity, but all fractions showed considerable immune activity

  18. Preparation and characterization of mucilage polysaccharide for biomedical applications.

    Science.gov (United States)

    Archana, G; Sabina, K; Babuskin, S; Radhakrishnan, K; Fayidh, Mohammed A; Babu, P Azhagu Saravana; Sivarajan, M; Sukumar, M

    2013-10-15

    In the present investigation, the polysaccharide/mucilage from waste of Abelmoscus esculentus by modification in hot extraction using two different solvents (Acetone, Methanol) were extracted, characterized and further compared with seaweed polysaccharide for their potential applications. The percentage yield, emulsifying capacity and swelling index of this mucilage were determined. The macro algae and okra waste, gave high % yield (22.2% and 8.6% respectively) and good emulsifying capacity (EC%=52.38% and 54.76% respectively) with acetone, compared to methanol (11.3% and 0.28%; EC%=50%) (PH=7) while swelling index was greater with methanol than acetone extracts respectively. The infrared (I.R.) spectrum of the samples was recorded to investigate the chemical structure of mucilage. Thermal analysis of the mucilage was done with TGA (Thermal Gravimetric Analyzer) and DSC (Differential Scanning Calorimeter) which showed both okra and algal polysaccharide were thermostable hydrogels.

  19. Antioxidant properties of cell wall polysaccharides of Stevia rebaudiana leaves

    Directory of Open Access Journals (Sweden)

    Mediesse Kengne Francine

    2014-12-01

    Full Text Available Objective: To examine the total phenolic and protein contents, and the antioxidant activities of cell wall polysaccharide fractions of Stevia rebaudiana leaves. Methods: Three different polysaccharide-enriched fractions, namely FPE (extract with 50 mmol/ L ethylene diamine tetra acetic acid, FPK (extract with 0.05 mol/L KOH and FH (extract with 4 mol/L KOH were extracted from Stevia rebaudiana leaves. The antioxidant activity of these fractions was evaluated based on their ability to scavenge DPPH (1, 1-diphenyl-2-picryl hydrazyl free radical, to reduce ferric power, to chelate ferrous ion and to protect human DNA. Results: The results indicated that protein content was found to be higher in FPK polysaccharide enriched fraction (47.48 µg per mg of FPK. Furthermore, the phenolic compound analysis according to the Folin-Ciocalteu method was higher in FPK (17.71 µg ferulic acid. The DPPH maximal inhibition percentage of the three polysaccharide-enriched fractions at 400 µg/mL was 27.66%, 59.90% and 23.21% respectively for FPE, FPK and FH. All the polysaccharide fractions exhibited a ferric reducing power except the FH one. The three fractions also exhibited lipid peroxidation inhibition, and they completely reverted the DNA damage induced by H2O2/FeCl2. FPK showed the strongest scavenging activity against the DPPH radical, the best chelating ability and lipid peroxidation inhibition. Conclusions: Stevia cell wall polysaccharide fractions are potent protective agents against oxidative stress. The analysis revealed major differences in the antioxidant activity in the three polysaccharides fractions. However, the 0.05 mol/L KOH pectin fraction (FPK showed better antioxidant activity.

  20. Antioxidant properties of cell wall polysaccharides of Stevia rebaudiana leaves

    Institute of Scientific and Technical Information of China (English)

    Mediesse Kengne Francine; Woguia Alice Louise; Fogue Souopgui Pythagore; Atogho-Tiedeu Barbara; Simo Gustave; Thadde Boudjeko

    2014-01-01

    Objective: To examine the total phenolic and protein contents, and the antioxidant activities of cell wall polysaccharide fractions of Stevia rebaudiana leaves.Methods:L ethylene diamine tetra acetic acid), FPK (extract with 0.05 mol/L KOH) and FH (extract with 4 mol/L KOH) were extracted from Stevia rebaudiana leaves. The antioxidant activity of these fractions was evaluated based on their ability to scavenge DPPH (1, 1-diphenyl-2-picryl hydrazyl) free radical, to reduce ferric power, to chelate ferrous ion and to protect human DNA. Three different polysaccharide-enriched fractions, namely FPE (extract with 50 mmol/Results: The results indicated that protein content was found to be higher in FPK polysaccharide enriched fraction (47.48 µg per mg of FPK). Furthermore, the phenolic compound analysis according to the Folin-Ciocalteu method was higher in FPK (17.71 µg ferulic acid). The DPPH maximal inhibition percentage of the three polysaccharide-enriched fractions at 400 µg/mL was 27.66%, 59.90% and 23.21% respectively for FPE, FPK and FH. All the polysaccharide fractions exhibited a ferric reducing power except the FH one. The three fractions also exhibited lipid peroxidation inhibition, and they completely reverted the DNA damage induced by H2O2/FeCl2. FPK showed the strongest scavenging activity against the DPPH radical, the best chelating ability and lipid peroxidation inhibition.Conclusions: Stevia cell wall polysaccharide fractions are potent protective agents against oxidative stress. The analysis revealed major differences in the antioxidant activity in the three polysaccharides fractions. However, the 0.05 mol/L KOH pectin fraction (FPK) showed better antioxidant activity.

  1. 不添加蛋白酶从百合根茎中提取分离纯化水溶性非淀粉多糖%Extraction, isolation and purification of water soluble non-starch polysaccharides from Lily bulb without protease treatment

    Institute of Scientific and Technical Information of China (English)

    Manal M Shehata; 王璋; 许时婴

    2004-01-01

    采用不添加蛋白酶的方法从百合根茎中提取分离水溶性非淀粉多糖(WSNSP).用离子交换色谱和凝胶过滤色谱纯化WSNSP,测定了WSNSP的相对分子质量,其值为97.8 kD.另外,研究发现一些多糖是与蛋白质结合在一起的.%Extraction, and isolation of water-soluble non-starch polysaccharides (WSNSP) from lily bulb without the use of protease was investigated. WSNSP was purified by ion exchange and gel filtration chromatography. Molecular weight was determined and found to be 97.8 kD Furthermore, some polysaccharides would be associated with proteins.

  2. 花果山墨旱莲多糖的提取及体外抗氧化活性比较%Extraction of Polysaccharides from Eclipata Alba Grown in Huaguo Mountain and Comparison of Antioxidant Activity in vitro with Total Flavonoids from Eclipata Alba

    Institute of Scientific and Technical Information of China (English)

    许瑞波; 王新新; 唐秋萍; 胡喜兰; 詹永成

    2011-01-01

    在单因素试验的基础上,通过正交试验优化从连云港花果山墨旱莲中提取多糖的工艺条件,并且对墨旱莲多糖(polysaccharides of Eclipata Alba,PEA)与墨旱莲黄酮类化合物(flavonoids of Eclipata Alba,FEA)的抗氧化活性进行比较。结果表明:PEA的最适宜提取工艺条件为料液比1:15、提取温度90℃、提取时间1h、提取3次;用Sevag法去除PEA中蛋白质、核酸等杂质,并用紫外光谱、红外光谱对其进行表征;最后,利用Fenton反应和邻苯三酚自氧化反应比较PEA、FEA对羟自由基(.OH)和超氧阴离子自由基(O-2.)的清除能力,结果表明PEA、FE A对两种自由基都具有良好的清除能力,而且对.O H的清除能力大于对O-2.的清除能力。当质量浓度大于0.085mg/mL时,FEA的抗氧化活性强于PEA。%Using one-factor-at-a-time experiments followed by orthogonal array design,the process conditions of polysaccharide extraction from Eclipata Alba grown in Huaguo Mountain were optimized in this study.Also,a comparison of antioxidant activity was carried out between polysaccharides(PEA) and flavonoids(FEA) from Eclipata Alba.The results showed that the optimum conditions for polysaccharide extraction were material-to-liquid ratio of 1:15(g/mL),extraction time of 1 h,extraction temperature of 90 ℃ and extraction number of 3.After the removal of impurities such as proteins,nucleic acids,etc,the extracted polysaccharides were characterized using ultraviolet spectroscopy and infrared spectroscopy.Both PEA and FEA had a powerful ability to scavenge hydroxyl and superoxide anion radicals and could scavenge the former more powerfully.At the same concentrations exceeding 0.085 mg/mL,FEA indicated stronger antioxidant activity than PEA.

  3. Isolation and structure elucidation of pectic polysaccharide from rose hip fruits (Rosa canina L.)

    NARCIS (Netherlands)

    Ognyanov, Manol; Remoroza, Connie; Schols, Henk A.; Georgiev, Yordan; Kratchanova, Maria; Kratchanov, Christo

    2016-01-01

    A pectic polysaccharide from rose hip (RH) fruits has been obtained by extraction with 1% aqueous citric acid. It was found that the polysaccharide fraction mainly consisted of galacturonic acid (45.5%) next to galactose (5.5%) and arabinose (4.7%). RH pectin is having a relatively high degree of

  4. Characterisation of antioxidant and antiproliferative acidic polysaccharides from Chinese wolfberry fruits

    NARCIS (Netherlands)

    He, N.W.; Yang, X.B.; Jiao, Y.D.; Tian, L.; Zhao, Y.

    2012-01-01

    Wolfberry fruit polysaccharides (WFPs) were isolated by hot-water extraction and ethanol precipitation. With HPLC analysis, WFPs were for the first time identified as acidic polysaccharides with galacturonic acid being the main component monosaccharide (24.9%), followed by galactose (21.3%), arabino

  5. Characterisation of antioxidant and antiproliferative acidic polysaccharides from Chinese wolfberry fruits

    NARCIS (Netherlands)

    He, N.W.; Yang, X.B.; Jiao, Y.D.; Tian, L.; Zhao, Y.

    2012-01-01

    Wolfberry fruit polysaccharides (WFPs) were isolated by hot-water extraction and ethanol precipitation. With HPLC analysis, WFPs were for the first time identified as acidic polysaccharides with galacturonic acid being the main component monosaccharide (24.9%), followed by galactose (21.3%),

  6. Isolation and structure elucidation of pectic polysaccharide from rose hip fruits (Rosa canina L.)

    NARCIS (Netherlands)

    Ognyanov, Manol; Remoroza, Connie; Schols, Henk A.; Georgiev, Yordan; Kratchanova, Maria; Kratchanov, Christo

    2016-01-01

    A pectic polysaccharide from rose hip (RH) fruits has been obtained by extraction with 1% aqueous citric acid. It was found that the polysaccharide fraction mainly consisted of galacturonic acid (45.5%) next to galactose (5.5%) and arabinose (4.7%). RH pectin is having a relatively high degree of

  7. Antihypertensive activity of polysaccharide from Crassostrea gigas.

    Science.gov (United States)

    Wang, Ting; Ding, Jie; Li, Haibo; Xiang, Jingjing; Wen, Ping; Zhang, Qin; Yin, Linliang; Jiang, Wei; Shen, Caie

    2016-02-01

    Water-soluble polysaccharide was extracted from Crassostrea gigas by hydrolysis with flavourzyme and filtered, ultrafiltered and precipitated using absolute ethanol. Sugar composition analysis performed on the C. gigas polysaccharide (CGP) by high performance liquid chromatography indicated that it was comprised primarily of glucose, and its molecular weight was determined using a TSK-GEL G5000PW column to be ∼3.413×10(6) Da. Next, the antihypertensive activity of CGP was evaluated in rats. Hypertension model Wistar rats were divided into three groups and intragastrically treated with physiological saline (negative control group), CGP (treatment group), and captopril (positive control group). CGP treatment led to significant decrease in both systolic and diastolic pressures in the hypertension model Wistar rats. Furthermore, the antihypertensive effect of CGP was comparable with that of captopril. Thus, CGP has antihypertensive effects and can potentially be used as a therapeutic agent for hypertension. Copyright © 2015 Elsevier B.V. All rights reserved.

  8. [Biological activities of exogenous polysaccharides via controlling endogenous proteoglycan metabolism in vascular endothelial cells].

    Science.gov (United States)

    Sato, Tomoko; Yamamoto, Chika; Fujiwara, Yasuyuki; Kaji, Toshiyuki

    2008-05-01

    Proteoglycan contains glycosmainoglycans, which are endogenous sulfated polysaccharides, in the molecule. The metabolism of proteoglycans regulates cell behavior and cellular events. It is possible that exogenous polysaccharide-related molecules exhibit their biological activities by two mechanisms. One is the interaction with cells and the other is the interaction with growth factors/cytokines that regulate proteoglycans. In this review, we describe sodium spirulan, a sulfated polysaccharide obtained from a hot-water extract of the blue-green alga Spirulina platensis, as an exogenous polysaccharide that stimulates the release of proteoglycans from vascular endothelial cells. Factors that regulate endothelial proteoglycan metabolism are also being described as possible target molecules of exogenous polysaccharides. Further research is required to obtain exogenous polysaccharide-related molecules that exhibit useful biological activities through controlling endothelial proteoglycan metabolism for protection against vascular lesions such as atheroslcerosis.

  9. The extraction of asparagus polysaccharide and its effect on the growth of human hepatic cancer SMMC-7721 cells in vitro%天冬多糖的提取及其对人肝癌SMMC-7721细胞生长影响的研究

    Institute of Scientific and Technical Information of China (English)

    张闽光; 陈刚; 刘力

    2011-01-01

    Objective To investigate the technical reliability of extracting polysaccharide from asparagus cochinchinensis and to discuss the effect of asparagus polysaccharide on the growth of a human hepatic carcinoma cell line. SMMC-7721 cells. in vitro. Methods The asparagus cochinchinensis was soaked in cold water and the crude asparagus polysaccharide was extracted with ethanol precipitation. Then,the crude asparagus polysaccharide was deproteinized with the method of pronase E and Sevag. Finally , the deproteinized asparagus polysaccharide was purified with the cellulose and sepharose chromatographic column, which was further identified and its molecular weight was determined. The effect of asparagus polysaccharide in different dose on the growth of the hepatic carcinoma SMMC-7721 cells was estimated with MTT assay in vitro, and the dose response curve as well as the growth curve was drawn. Results The extracted substance obtained by authors' method was further purified and deproteinized, and asparagus polysaccharide was thus gained. Its molecular weight ranged from 5 000 to 400 000 u. It had a twofold regulative effect on the growth of the hepatic carcinoma SMMC-7721 cells. When its concentration was lower,below 800 μg/ml, the asparagus polysaccharide had the effect of promoting the vegetation of the hepatic carcinoma SMMC-7721 cells. However. when its concentration was higher, over 900 μg/ml, the asparagus polysaccharide showed certain inhihitory effect on the vegetation of SMMC-7721 cells. besides,with the time passing by and the concentration increasing, the inhibitory effect became more effective ( P < 0.05 ). Conclusion The results of this experiment indicate that authors' method to extract, separate and purify the asparagus polysaccharide is technically reliable. In vitro, low concentration of asparagus polysaccharide can promote, while high concentration of asparagus polysaccharide can suppress, the vegetation of the SMMC-7721 cells, and the effectiveness is

  10. Antioxidant activity of herbal polysaccharides and cough reflex.

    Science.gov (United States)

    Nosalova, G; Jurecek, L; Hromadkova, Z; Kostalova, Z; Sadlonova, V

    2013-01-01

    The extraction of Fallopia sachalinensis leaves resulted in two fractions (FS-1 and FS-2). Chemical and spectral analyses of samples revealed the prevalence of pectic polysaccharides with high galacturonic acid, arabinose, galactose, and rhamnose content. Arabinogalactan with a higher content of phenolic prevailed in the FS-1, whereas rhamnogalacturonan predominated in the FS-2 fraction. Both polysaccharides showed significant antioxidant activity according to DPPH and FRAP assays. Evaluation of antitussive activity in healthy adult conscious guinea pigs after oral application of 50 and 75 mg/kg of the FS-2 polysaccharide extracts showed a significant suppression of cough reflex, without an influence on specific airway resistance. The suppression of cough was comparable with that of codeine.

  11. Marine derived polysaccharides for biomedical applications: chemical modification approaches.

    Science.gov (United States)

    d'Ayala, Giovanna Gomez; Malinconico, Mario; Laurienzo, Paola

    2008-09-03

    Polysaccharide-based biomaterials are an emerging class in several biomedical fields such as tissue regeneration, particularly for cartilage, drug delivery devices and gelentrapment systems for the immobilization of cells. Important properties of the polysaccharides include controllable biological activity, biodegradability, and their ability to form hydrogels. Most of the polysaccharides used derive from natural sources; particularly, alginate and chitin, two polysaccharides which have an extensive history of use in medicine, pharmacy and basic sciences, and can be easily extracted from marine plants (algae kelp) and crab shells, respectively. The recent rediscovery of poly-saccharidebased materials is also attributable to new synthetic routes for their chemical modification, with the aim of promoting new biological activities and/or to modify the final properties of the biomaterials for specific purposes. These synthetic strategies also involve the combination of polysaccharides with other polymers. A review of the more recent research in the field of chemical modification of alginate, chitin and its derivative chitosan is presented. Moreover, we report as case studies the results of our recent work concerning various different approaches and applications of polysaccharide-based biomaterials, such as the realization of novel composites based on calcium sulphate blended with alginate and with a chemically modified chitosan, the synthesis of novel alginate-poly(ethylene glycol) copolymers and the development of a family of materials based on alginate and acrylic polymers of potential interest as drug delivery systems.

  12. Marine Derived Polysaccharides for Biomedical Applications: Chemical Modification Approaches

    Directory of Open Access Journals (Sweden)

    Paola Laurienzo

    2008-09-01

    Full Text Available Polysaccharide-based biomaterials are an emerging class in several biomedical fields such as tissue regeneration, particularly for cartilage, drug delivery devices and gelentrapment systems for the immobilization of cells. Important properties of the polysaccharides include controllable biological activity, biodegradability, and their ability to form hydrogels. Most of the polysaccharides used derive from natural sources; particularly, alginate and chitin, two polysaccharides which have an extensive history of use in medicine, pharmacy and basic sciences, and can be easily extracted from marine plants (algae kelp and crab shells, respectively. The recent rediscovery of poly-saccharidebased materials is also attributable to new synthetic routes for their chemical modification, with the aim of promoting new biological activities and/or to modify the final properties of the biomaterials for specific purposes. These synthetic strategies also involve the combination of polysaccharides with other polymers. A review of the more recent research in the field of chemical modification of alginate, chitin and its derivative chitosan is presented. Moreover, we report as case studies the results of our recent work concerning various different approaches and applications of polysaccharide-based biomaterials, such as the realization of novel composites based on calcium sulphate blended with alginate and with a chemically modified chitosan, the synthesis of novel alginate-poly(ethylene glycol copolymers and the development of a family of materials based on alginate and acrylic polymers of potential interest as drug delivery systems.

  13. Health benefits of algal polysaccharides in human nutrition.

    Science.gov (United States)

    Mišurcová, Ladislava; Škrovánková, Soňa; Samek, Dušan; Ambrožová, Jarmila; Machů, Ludmila

    2012-01-01

    The interest in functional food, both freshwater and marine algal products with their possible promotional health effects, increases also in regions where algae are considered as rather exotic food. Increased attention about algae as an abundant source of many nutrients and dietary fiber from the nutrition point of view, as well as from the scientific approaches to explore new nutraceuticals and pharmaceuticals, is based on the presence of many bioactive compounds including polysaccharides extracted from algal matter. Diverse chemical composition of dietary fiber polysaccharides is responsible for their different physicochemical properties, such as their ability to be fermented by the human colonic microbiota resulted in health benefit effects. Fundamental seaweed polysaccharides are presented by alginates, agars, carrageenans, ulvanes, and fucoidans, which are widely used in the food and pharmaceutical industry and also in other branches of industry. Moreover, freshwater algae and seaweed polysaccharides have emerged as an important source of bioactive natural compounds which are responsible for their possible physiological effects. Especially, sulfate polysaccharides exhibit immunomodulatory, antitumor, antithrombotic, anticoagulant, anti-mutagenic, anti-inflammatory, antimicrobial, and antiviral activities including anti-HIV infection, herpes, and hepatitis viruses. Generally, biological activity of sulfate polysaccharides is related to their different composition and mainly to the extent of the sulfation of their molecules. Significant attention has been recently focused on the use of both freshwater algae and seaweed for developing functional food by reason of a great variety of nutrients that are essential for human health.

  14. Liposome-Based Delivery Systems in Plant Polysaccharides

    Directory of Open Access Journals (Sweden)

    Meiwan Chen

    2012-01-01

    Full Text Available Plant polysaccharides consist of many monosaccharide by α- or β-glycosidic bond which can be extracted by the water, alcohol, lipophile liquid from a variety of plants including Cordyceps sinensis, astragalus, and mushrooms. Recently, many evidences illustrate that natural plant polysaccharides possess various biological activities including strengthening immunity, lowering blood sugar, regulating lipid metabolism, antioxidation, antiaging, and antitumour. Plant polysaccharides have been widely used in the medical field due to their special features and low toxicity. As an important drug delivery system, liposomes can not only encapsulate small-molecule compound but also big-molecule drug; therefore, they present great promise for the application of plant polysaccharides with unique physical and chemical properties and make remarkable successes. This paper summarized the current progress in plant polysaccharides liposomes, gave an overview on their experiment design method, preparation, and formulation, characterization and quality control, as well as in vivo and in vitro studies. Moreover, the potential application of plant polysaccharides liposomes was prospected as well.

  15. Characterization and antitumor activity of a polysaccharide from Sarcodia ceylonensis.

    Science.gov (United States)

    Fan, Yijun; Lin, Mengchuan; Luo, Aoshuang; Chun, Ze; Luo, Aoxue

    2014-07-25

    A water-soluble polysaccharide from Sarcodia ceylonensis was obtained by using the method of water-extraction and ethanol-precipitation. The polysaccharide was further purified by chromatography on AB-8 and ADS-7 columns, yielding a pure polysaccharide termed SCP-60. The molecular weight (Mw) of SCP-60 was calculated to be 50.0 kDa, based on the calibration curve obtained with a series of Dextran T standards. The results of FT-IR indicated that the polysaccharide contains the α-configuration of sugar units. GC-MS analysis revealed that SCP-60 was mainly composed of galactose and glucose. NMR spectroscopy revealed SCP-60 had the backbone consisting of → 6)-α-Manp-(1 →, α-D-Glcp-(1 →, → 6)-α-D-Glcp-(1 → and → 6)-α-Galp-(1 →. In order to evaluate the antitumor activity in vivo of the polysaccharide, a sarcoma 180 model was used. The results showed SCP-60 had strong antitumor ability, meanwhile, SCP-60 at a high dose (100 mg/kg) could significantly increase the thymic and splenic indices of S180 mice, and strongly promote the secretion of IL-2, TNF-α and IFN-γ, increase the SOD activities and reduce the concentrations of MDA in blood. Therefore the polysaccharide SCP-60 should be explored as a novel potential antitumor drug.

  16. 超声提取鼠尾草叶多糖工艺优化及其DPPH自由基清除能力评价%Optimization of Ultrasonic Extraction of Polysaccharides from Salvia officinalis L. Leaves and Their DPPH Free Radical Scavenging Activity

    Institute of Scientific and Technical Information of China (English)

    祝元婷; 吴文林; 张利; 刘琪

    2011-01-01

    The conditions for ultrasonic extraction of polysaccharides from Salvia officinalis L.leaves were optimized by one-factor-at-a-time and orthogonal array design methods.A material-to-liquid ratio of 1:40 for 7 min extractions performed twice proved optimal.The resultant polysaccharide yield was 3.27%.The polysaccharide extract obtained had excellent DPPH free radical scavenging effect,revealing a scavenging rate of 82% at the concentration of 160μg/mL and an IC50 of 112μg/mL.%以鼠尾草(Salvia officinalis L.)叶为材料,采用单因素试验和正交试验,探讨超声提取其多糖的最佳工艺条件,并利用1,1-二苯基苦基苯肼(DPPH)自由基清除法评价该多糖的自由基清除能力。结果表明:超声提取多糖的最佳工艺条件为:提取时间7min、提取2次、料液比1:40,在此条件下鼠尾草叶多糖的提取率为3.27%。同时该多糖对DPPH自由基有较好的清除作用,当质量浓度为160μg/mL时,清除率达到82%,并且对DPPH自由基清除能力IC50为112μg/mL。

  17. Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities.

    Science.gov (United States)

    Ferreira, Isabel C F R; Heleno, Sandrina A; Reis, Filipa S; Stojkovic, Dejan; Queiroz, Maria João R P; Vasconcelos, M Helena; Sokovic, Marina

    2015-06-01

    Ganoderma genus comprises one of the most commonly studied species worldwide, Ganoderma lucidum. However, other Ganoderma species have been also reported as important sources of bioactive compounds. Polysaccharides are important contributors to the medicinal properties reported for Ganoderma species, as demonstrated by the numerous publications, including reviews, on this matter. Yet, what are the chemical features of Ganoderma polysaccharides that have bioactivity? In the present manuscript, the chemical features of Ganoderma polysaccharides with reported antioxidant, antitumor and antimicrobial activities (the most studied worldwide) are analyzed in detail. The composition of sugars (homo- versus hetero-glucans and other polysaccharides), type of glycosidic linkages, branching patterns, and linkage to proteins are discussed. Methods for extraction, isolation and identification are evaluated and, finally, the bioactivity of polysaccharidic extracts and purified compounds are discussed. The integration of data allows deduction of structure-activity relationships and gives clues to the chemical aspects involved in Ganoderma bioactivity.

  18. [Study on totai flavonoids of Epimedium assisted with soybean polysaccharide spray-drying powder].

    Science.gov (United States)

    Yan, Hong-mei; Jia, Xiao-bin; Zhang, Zhen-hai; Sun, E; Deng, Jia-hui

    2015-08-01

    In order to evaluate the characteristics of the spray drying of total flavonoids of Epimedium extracts assisted with soybean polysaccharide, a certain percentage of soybean polysaccharide or polyvidone were added to the total flavonoids of Epimedium extract to conduct the spray drying. The effect of soybean polysaccharides against the wall sticking effect of the spray drying was detected, as well as the powder property of total flavonoids of Epimedium spray drying powder and the dissolution in vitro behavior of the effective component. Compared with the total flavonoids of Epimedium spray drying powder, soybean polysaccharide revealed a significant anti-wall sticking effect. The spray drying power which had no notable change in the grain size made a increase in the fluidity, improvement in the moisture absorption and remarkable rise in the dissolution in vitro behavior. It was worth further studying the application of soybean polysaccharide in spray drying power of traditional Chinese medicine.

  19. Penggunaan Ekstrak Kasar Polisakarida Larut Air dari Biji Buah Durian (Durio zibethinus Murr. pada Pembuatan Saos Tomat (The Use of Crude Extract Water Soluble Polysaccharide from Durian (Durio Zibethinus Muur. Seeds in Tomato Sauce Processing

    Directory of Open Access Journals (Sweden)

    Herlina Herlina

    2016-12-01

    Full Text Available Tomato sauce is one of the diversification of tomato products that could increase the added value and shelf life of tomatoes. Sauce made from tomato has a high water content (94 % resulting a watery sauce product. The purpose of this study was to determine the effect of crude extract Water Soluble Polysaccharide (WSP from durian seeds on physical, chemical, and organoleptic as characteristics oftomato sauce. The use of crude extract WSP from durian seeds is expected to increase the viscosity of the sauce. The experimental design used in this study was a non factorial randomized complete block design with three replications. Parameters measured were viscosity, color (Hue and Chroma, total dissolved solids, pH, vitamin C, and organoleptic (panelist preferences in addition to the effectiveness test to determine the best treatment. The results show that the tomato sauce composition significantly affect the viscosity, pH, color intensity, and total dissolved solids, while no significant effect on the angle of color and vitamin C of tomato sauce. The effectiveness test showed that the panelists preferred the P4 tomato sauce (use crude extract WSP from seeds fruit of durian 0.8 % with fondness value  color of 2.68; aroma of 3.08; flavor of 3.48; viscosity of 3.44; overall liking of 3.56. The value of viscosity, hue, chroma, total dissolved solids, pH, vitamin C were 39.64 centipoise; 38,34°; 20.16; 35° Brix; 4.92; and 4.95 mg/g, respectively.   ABSTRAK Saos tomat merupakan salah satu diversifikasi produk olahan buah tomat yang dapat meningkatkan nilai tambah dan umur simpan buah tomat. Saos yang berbahan dasar tomat, memiliki kandungan air yang tinggi (94 % sehingga menghasilkan saos yang encer. Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan ekstrak kasar Polisakarida Larut Air (PLA dari biji buah durian terhadap sifat fisiko kimia dan organoleptik saos tomat. Penggunaan ekstrak kasar PLA dari biji buah durian diharapkan dapat

  20. Therapeutic role of glucogalactan polysaccharide extracted from ...

    African Journals Online (AJOL)

    RACHEL

    Article Number: 5369C7953712. ISSN 1684-5315 .... The number of mushrooms with known pharmaco- ..... The cell suspension was mixed with an equal volume of 1% ..... was noticed also in between the neuronal cells of the hippocampus.(f).

  1. [Optimum of polysaccharide distillation on scrap Cordyceps militaris medium].

    Science.gov (United States)

    Ren, Shu-Yu; Zhao, Chun-Yan; Song, Hui-Yi; Zhao, Hao-Lu; Sun, Jun-De

    2008-03-01

    A mass of scrap Cordyceps militaris solid culture medium could not be utilized better. In this test, using orthogonal design the optimal technique parmeter of extracting polysaccharide was 80 degrees C, two times, in twenty times of water, and 120 minutes each time. Temperature was the most important factor. The referenced data could be provided to depurative production of Cordyceps militaris and resource utilization.

  2. Structure of pectic polysaccharides from sunflower salts-soluble fraction

    Science.gov (United States)

    The manuscript discusses the structural features of pectin polysaccharides extracted from seedless sunflower head residues. The analysis using 1H, 13C and two-dimensional gHSQC NMR showed various numbers of methyl and hydroxyl groups attached to the anomeric carbons in the pectin backbone at differe...

  3. Effects of mushroom-derived ß-glucan rich polysaccharide extracts on nitric oxide production by bone marrow-derived macrophages and nuclear factor-kB transactivation in Caco-2 reporter cells: Can effects be explained by structure?

    NARCIS (Netherlands)

    Volman, J.J.; Helsper, J.P.F.G.; Wei, S.; Baars, J.J.P.; Griensven, van L.J.L.D.; Sonnenberg, A.S.M.; Mensink, R.P.; Plat, J.

    2010-01-01

    Mushrooms are known for their immune-modulating and anti-tumour properties. The polysaccharide fraction, mainly -glucans, is responsible for the immune-modulating effects. Fungal -glucans have been shown to activate leukocytes, which depend on structural characteristics of -glucans. As edible mushro

  4. Process optimization on extracting of selenium polysaccharides with ultrasonic and microwave method from selenium-enriched Auricularia auricula and its antioxidant activity%富硒黑木耳硒多糖超声-微波提取工艺优化及其抗氧化活性

    Institute of Scientific and Technical Information of China (English)

    刘春延; 赵博; 张国财; 郑煜; 李建顺; 刘丛荣

    2016-01-01

    以富硒黑木耳中的硒多糖为考察指标,采用超声–微波提取法,分别设超声时间、微波时间、超声功率、微波功率、料液比5个单因素试验,再选择对富硒黑木耳中硒多糖提取率有显著影响的4个因素(超声时间、微波时间、微波功率和料液比)进行响应面分析,优化富硒黑木耳硒多糖的提取工艺条件。结果表明:采用超声–微波提取富硒黑木耳中硒多糖,在超声时间26 min、微波时间22 min、微波功率350 W 以及料液比1∶58(g/mL)时,硒多糖提取率最高,为11.79%,与传统水提法相比,提高了4.1%,提取时间缩短了56.67%。以传统水提法提取的富硒黑木耳中硒多糖为对照,进一步研究超声–微波提取富硒黑木耳中硒多糖的总还原力以及对羟基自由基和DPPH 自由基的清除率,结果表明,超声–微波提取的富硒黑木耳硒多糖和传统水提法提取的硒多糖的还原力吸光值分别为0.431和0.410,对羟基自由基清除的半抑制浓度(IC50)值分别为3.81、4.91 mg/mL,对 DPPH 自由基清除的 IC50值分别为4.59、5.70 mg/mL,说明超声–微波提取的富硒黑木耳硒多糖抗氧化活性优于传统水提法提取的硒多糖。%Selenium polysaccharides extracting from selenium-enriched Auricularia auricula by the ultrasonic and microwave method was used as an index in this test. The effects of different ultrasonic time, microwave time, ultrasonic power, microwave power and ratio of liquor to material on extracting rate of selenium polysaccharides were investigated by single factor experiment, then ultrasonic time, microwave time, microwave power and ratio of liquor to material, the four factors which had significant effects on extraction rate of selenium polysaccharides were chosen to determine the optimum process parameters in response surface test. The results showed that under the conditions of 1∶58 (g/mL) of ratio of liquor to

  5. Polysaccharides of the red algae.

    Science.gov (United States)

    Usov, Anatolii I

    2011-01-01

    Red algae (Rhodophyta) are known as the source of unique sulfated galactans, such as agar, agarose, and carrageenans. The wide practical uses of these polysaccharides are based on their ability to form strong gels in aqueous solutions. Gelling polysaccharides usually have molecules built up of repeating disaccharide units with a regular distribution of sulfate groups, but most of the red algal species contain more complex galactans devoid of gelling ability because of various deviations from the regular structure. Moreover, several red algae may contain sulfated mannans or neutral xylans instead of sulfated galactans as the main structural polysaccharides. This chapter is devoted to a description of the structural diversity of polysaccharides found in the red algae, with special emphasis on the methods of structural analysis of sulfated galactans. In addition to the structural information, some data on the possible use of red algal polysaccharides as biologically active polymers or as taxonomic markers are briefly discussed. Copyright © 2011 Elsevier Inc. All rights reserved.

  6. Antioxidant and Immunoregulatory Activity of Polysaccharides from Quinoa (Chenopodium quinoa Willd.)

    OpenAIRE

    Yang Yao; Zhenxing Shi; Guixing Ren

    2014-01-01

    The water-extractable (QWP) and the alkali-extractable (QAP) polysaccharides from quinoa (named QWP and QAP, respectively) and their four polysaccharide sub-fractions (QWP-1, QWP-2, QAP-1 and QAP-2), were isolated and purified by anion-exchange and gel filtration chromatography. QWP-1 and QWP-2 were composed of Rha, Ara, Gal and GalA. QAP-1 and QAP-2 were composed of Rha, Ara, Man, Gal and GalA. Antioxidant and immunoregulatory activities of the polysaccharides were evaluated. The results s...

  7. EXTRACT

    DEFF Research Database (Denmark)

    Pafilis, Evangelos; Buttigieg, Pier Luigi; Ferrell, Barbra

    2016-01-01

    The microbial and molecular ecology research communities have made substantial progress on developing standards for annotating samples with environment metadata. However, sample manual annotation is a highly labor intensive process and requires familiarity with the terminologies used. We have the...... and text-mining-assisted curation revealed that EXTRACT speeds up annotation by 15-25% and helps curators to detect terms that would otherwise have been missed.Database URL: https://extract.hcmr.gr/....