WorldWideScience

Sample records for k-4 videotape history

  1. Look and Do Ancient Egypt. Teacher's Manual: Primary Program, Ancient Egypt Art & Architecture [and] Workbook: The Art and Architecture of Ancient Egypt [and] K-4 Videotape. History through Art and Architecture.

    Science.gov (United States)

    Luce, Ann Campbell

    This resource contains a teaching manual, reproducible student workbook, and color teaching poster, which were designed to accompany a 2-part, 34-minute videotape, but may be adapted for independent use. Part 1 of the program, "The Old Kingdom," explains Egyptian beliefs concerning life after death as evidenced in art, architecture and…

  2. History from K-4: What Can We Really Do?

    Science.gov (United States)

    Klee, Mary Beth

    1998-01-01

    Outlines the characteristics of a good history program for kindergarten through grade four, analyzing the Massachusetts History and Social Science Curriculum Framework and the Core Knowledge Sequence as they apply to elementary instruction. Also describes and evaluates principles of teaching history to young children. (Author/SLD)

  3. Scars. [Videotape].

    Science.gov (United States)

    2002

    This 40-minute videotape tackles the issue of childhood bullying and unwanted teasing and torment. This videotape features real school children handling dramatic roles, and "doing the right thing" (aka "positive modeling.") The film is divided into two distinct parts: first act themes include bullying, girl bullies, children without one or both…

  4. Space Science in Action: Space Exploration [Videotape].

    Science.gov (United States)

    1999

    In this videotape recording, students learn about the human quest to discover what is out in space. Students see the challenges and benefits of space exploration including the development of rocket science, a look back at the space race, and a history of manned space travel. A special section on the Saturn V rocket gives students insight into the…

  5. Biochemical Engineering Education through Videotapes.

    Science.gov (United States)

    Austin, G. D.; And Others

    1990-01-01

    The implementation and results of the use of videotapes in introductory biochemical engineering classes are discussed. The topics of the videotapes and the results of a questionnaire about this instructional method are presented. (CW)

  6. Earthquake and Schools. [Videotape].

    Science.gov (United States)

    Federal Emergency Management Agency, Washington, DC.

    Designing schools to make them more earthquake resistant and protect children from the catastrophic collapse of the school building is discussed in this videotape. It reveals that 44 of the 50 U.S. states are vulnerable to earthquake, but most schools are structurally unprepared to take on the stresses that earthquakes exert. The cost to the…

  7. Parent's Journal. [Videotape Series].

    Science.gov (United States)

    1999

    Parent's Journal is a set of 16 videotapes for parents of prenatal, infant, and toddler-age children, created by the Alaska Native Home Base Video Project of the Tlingit and Haida Head Start Program. This series offers culturally relevant solutions to the challenges of parenting, drawing on the life stories and experiences of capable mothers and…

  8. Family Feathers. [Videotape Series].

    Science.gov (United States)

    1999

    Family Feathers is a set of 18 videotapes for parents of preschool children, created by the Alaska Native Home Base Video Project of the Tlingit and Haida Head Start Program. This series offers culturally relevant solutions to the challenges of parenting, drawing on practical advice from Tlingit and Haida parents, wisdom from elders, and some of…

  9. Videotaping Students' Booktalks.

    Science.gov (United States)

    Martin, Ron

    1987-01-01

    Provides a detailed description of a class project in which each student is videotaped while presenting a booktalk. Included are a list of appropriate books, a checklist for students to use in identifying the important characteristics of a booktalk, and an evaluation form for presentations. (CLB)

  10. Space Science for Children: All about the Sun [Videotape].

    Science.gov (United States)

    1999

    This 23-minute videotape aims to give children, grades K-4, a broad understanding of the center of our solar system, the sun. It explains how the sun provides us with life-giving light and heat, how it's responsible for our seasons and weather, and why it's the primary source of energy on Earth. A hands-on activity in which children create their…

  11. Videotaped Lectures in a Graduate Cytogenetics Course.

    Science.gov (United States)

    Phillips, R. L.; Jellen, E. N.

    1994-01-01

    Graduate students evaluated the use of videotape recordings of lectures on chromosome configurations in a cytogenetics course. Ninety-two percent of the students indicated that videotaping was worthwhile. Advantages for using the videotaped cytogenetics lectures are presented. (MDH)

  12. Unifiability in extensions of K4

    NARCIS (Netherlands)

    Gencer, Ç.; de Jongh, D.

    2009-01-01

    We extend and generalize the work on unifiability of [8]. We give a semantic characterization for unifiability and non-unifiability in the extensions of K4. We apply this in particular to extensions of KD4, GL and K4.3 to obtain a syntactic characterization and give a concrete decision procedure for

  13. Consolidated Incineration Facility model videotape

    Energy Technology Data Exchange (ETDEWEB)

    Krolewski, J F; Augsburger, S T

    1988-01-01

    A Consolidated Incineration Facility (CIF) is in final design for construction at the Savannah River Plant in Aiken, South Carolina. The CIF will detoxify and volume reduce combustible radioactive, hazardous and mixed waste. A study model was constructed during scope development for project authorization to assist with equipment layout and insure sufficient maintenance access. To facilitate the Department of Energy Validation process, a videotape of the model was developed. This ten minute videotape includes general information about the incineration process and a tour of the study model with a discussion of activities in each area. The videotape will be shown and the current status and schedule for the CIF presented.

  14. Me & Isaac Newton. [Videotape and] KIDSNET Guide for Educators.

    Science.gov (United States)

    2001

    This videotape records the stories of 7 scientists, 3 women and 4 men who range in age from 33 to 81. Beginning with their earliest scientific questions and including their most personal ponderings, the scientists reveal their histories and professional obligations to affect the world. Director Michael Apted allows the personal adventures of the…

  15. Teaching Strategies for K-4 General Music.

    Science.gov (United States)

    Teaching Music, 1999

    1999-01-01

    Provides a strategy, from the book entitled "Strategies for Teaching K-4 General Music," that addresses Standard 6A of the National Standards for Music Education. Explains that students will identify characteristics that enables them to make decisions about the form of the composition in listening examples longer than classroom songs. (CMK)

  16. ESSEA K-4 Online Course: Polar Connections

    Science.gov (United States)

    Blaney, L.; Myers, R. J.; Schwerin, T.

    2007-12-01

    The Earth System Science Education Alliance (ESSEA) is a National Science Foundation-supported program implemented by the Institute for Global Environmental Strategies (IGES) to improve the quality of geoscience instruction for pre-service, middle, and high school teachers. ESSEA increases teachers' access to quality materials, standards-based instructional methods and content knowledge. Started in 2000 and based on a trio of online courses (for elementary, middle, and high school teachers), the courses have been used by 40 faculty at 20 institutions educating over 1,700 teachers in Earth system science. Program evaluation of original course participants indicated that the courses had significant impact on teachers Earth system content knowledge and beliefs about teaching and learning. Seventeen of the original participating institutions have continued to use the courses and many have developed new programs that incorporate the courses in Earth science education opportunities for teachers. Today the ESSEA program lists nearly 40 colleges and universities as participants. The original K-4 course and modules have been revised to include topics and resources focusing on the International Polar Year. The new K-4 Land, Living Things, Water and Air modules contain inquiry-based investigations exploring our polar regions. Each module lists a set of essential questions that guide teachers and their students as they build content knowledge. The course structure requires teachers to work individually and in teams to build content knowledge and pedagogical understanding of how their students learn. This group investigation approach and a "Teacher as Researcher" theme promote reflection and collaboration to develop criteria for effective concept building. By exploring the characteristics of polar landscapes, atmosphere, and polar life, teachers and their students will develop new understandings about the interactions and dependencies of the Earth spheres and our polar regions

  17. Videotapes

    CERN Document Server

    Chavanne, A

    1989-01-01

    1666 : impact de la creation de l'Academie des Sciences par Colbert, trente ans apres le proces de Galile, et au moment des disparitions de Pascal, Descartes et Fermat. Elle dirigee par le hollandais Huyggens jusqu'a sa fuite de France au moment de la revocation de l'Edit de Nantes. - 1750 : l'Encyclopedie (ou "Dictionnaire raisonne des Sciences, des Arts et des Metiers") de Diderot et d'Alembert, soutenus par Malherbes, Buffon, Condorcet et Rousseau. - 1789 : Revolution francaise. - 8 aout 1793 : l'Assemblee, par une declaration de Marat, dissout l'Academie des Sciences. Celle-ci continue cependant ses travaux pour les poids et mesures jusqu'en 1795. - la Terreur : la condamnation a mort, pas au nom d'une "Revolution qui n'a pas besoin de savants", de trois grands hommes de science : Lavoisier, Bailly et Condorcet. - 1793-1794 : Au printemps 93, le Comite de Salut Publique s'inquiete du demi-million de soldats etrangers de toutes les pays frontaliers qui essaient de penetrer en France pour occuper le pays. C...

  18. Using Videotape Technology: Innovations in Behavioral Research.

    Science.gov (United States)

    Niebuhr, Robert E.; And Others

    1981-01-01

    Reviews present and potential uses of videotape technology in behavioral research. Emphasis is placed on research methodology and the value of incorporating videotape in current research practices, including behavior observation studies, in modeling research, and in perceptual attribution investigations. Concludes with an analysis of advantages…

  19. Videotaping as a Counseling Technique with Families.

    Science.gov (United States)

    Getz, Hildy G.; Nininger, Kathleen

    1999-01-01

    Videotape and audiotape can be used very effectively to give counselees feedback, yet this method has received little attention in the literature. Describes a videotaping approach that can be used to enhance communication and resolve problems between troubled children and their families. Presents case example of a 10-year-old boy, threatening to…

  20. Developing Instructional Videotapes Step by Step.

    Science.gov (United States)

    Sweet, Thomas E.

    1990-01-01

    Discusses the eight steps in developing an instructional videotape: planning, brainstorming content, sequencing the storyline, defining the treatment, developing the introduction and conclusion, scripting the video and audio, controlling the production, and specifying the postproduction. (DMM)

  1. Parity, cycle space, and K4-subdivisions in graphs

    DEFF Research Database (Denmark)

    Thomassen, Carsten

    1999-01-01

    . Zang). We prove the new result that every $4$-connected graph with at least three triangles contains a totally odd $K_4$-subdivision if and only if it does notcontain a vertex whose deletion results in a bipartite graph.In particular, every $4$-connected planar graph contains a totally odd $K_4...

  2. Peaked and Smooth Solitons for K*(4,1 Equation

    Directory of Open Access Journals (Sweden)

    Yongan Xie

    2013-01-01

    Full Text Available This paper is contributed to explore all possible single peak solutions for the K*(4,1 equation ut=uxu2+2α(uuxxx+2uxuxx. Our procedure shows that the K*(4,1 equation either has peakon, cuspon, and smooth soliton solutions when sitting on a nonzero constant pedestal limξ→±∞u=A≠0 or possesses compacton solutions only when limξ→±∞u=A=0. We present a new smooth soliton solution in an explicit form. Mathematical analysis and numeric graphs are provided for those soliton solutions of the K*(4,1 equation.

  3. Teaching Mathematics with Computer Animated Videotapes.

    Science.gov (United States)

    Apostol, Tom M.

    1991-01-01

    Described are some of the visualization techniques employed in the computer-animated videotapes generated through the blending of commentary, animated imagery, music, and special effects. Instructions for procurement are included for these tapes, which are produced by Project MATHEMATICS! at the California Institute of Technology. (JJK)

  4. Cold atom microtraps above a videotape surface

    CERN Document Server

    Retter, J A

    2002-01-01

    Much progress has been made in the last two years towards miniaturizing magnetic traps for cold atoms. This will enable manipulation of coherent samples of atoms, such as Bose-Einstein condensates, or single atoms, on the scale of the atomic de Broglie wavelength. This thesis concerns an array of microscopic magnetic potentials formed close to the surface of magnetized videotape, when a uniform bias field is applied. The recorded magnetization is a 100 mu m sine wave, which covers a 12.5mm x 22mm piece of commercial videotape. This videotape is glued flat onto a thin glass substrate and is gold coated by evaporation so that atoms can be trapped close to the surface in a mirror-MOT. An 'atom chip' has been developed, incorporating the videotape and current-carrying wires located below the magnetized surface. A single wire and bias field create a magnetic tube potential, oriented parallel with the microtraps and with a quadrupole radial field. This allows the mirror-MOT to be compressed and distorted in order t...

  5. Producing videotapes as professional marketing tools.

    Science.gov (United States)

    Hess, L J; Harlan, N; Dyer, E; Lubker, B

    1992-05-01

    Professional quality videotapes are an effective way to reach audiences. Developing such tapes takes careful planning, time, and money, but they are well worth the effort involved. Our new role as "marketing agent" must be played with professionalism, expertise, persistence, and a sense of humor.

  6. Motion Picture and Videotape Analysis of Behavior.

    Science.gov (United States)

    Carpenter, Geoffrey C.; Duvall, David

    1983-01-01

    Use of motion pictures and videotape recordings to analyze animal behavior is described. Indicates that accuracy/amount of data available is greatly increased and that simultaneous behaviors of different animals can be studied or individual behavior patterns increased/decreased, providing observers with temporal perceptions similar to the animals…

  7. "Yes! Animation Is Possible with Your Videotape Recorder!"

    Science.gov (United States)

    Martino, Alfreda; Martin, Ron

    1987-01-01

    Describes a project in which students are involved in producing an animated videotape recording and discusses the advantages of modern videotape equipment over other film media. An outline of the process covers materials used, storyboard and artwork production, videotaping procedures, and audio dubbing. (CLB)

  8. Phosphorus K4 Crystal: A New Stable Allotrope

    Science.gov (United States)

    Liu, Jie; Zhang, Shunhong; Guo, Yaguang; Wang, Qian

    2016-11-01

    The intriguing properties of phosphorene motivate scientists to further explore the structures and properties of phosphorus materials. Here, we report a new allotrope named K4 phosphorus composed of three-coordinated phosphorus atoms in non-layered structure which is not only dynamically and mechanically stable, but also possesses thermal stability comparable to that of the orthorhombic black phosphorus (A17). Due to its unique configuration, K4 phosphorus exhibits exceptional properties: it possesses a band gap of 1.54 eV which is much larger than that of black phosphorus (0.30 eV), and it is stiffer than black phosphorus. The band gap of the newly predicted phase can be effectively tuned by appling hydrostastic pressure. In addition, K4 phosphorus exibits a good light absorption in visible and near ultraviolet region. These findings add additional features to the phosphorus family with new potential applications in nanoelectronics and nanomechanics.

  9. Properties of atomic intercalated carbon K4 crystals

    OpenAIRE

    Itoh, Masahiro; Takami, Seiichi; Kawazoe, Yoshiyuki; Adschiri, Tadafumi

    2009-01-01

    The stability of atomic intercalated carbon $K_{4}$ crystals, XC$_{2}$ (X=H, Li, Be, B, C, N, O, F, Na, Mg, Al, Si, P, S, Cl, K, Ca, Ga, Ge, As, Se, Br, Rb or Sr) is evaluated by geometry optimization and frozen phonon analysis based on first principles calculations. Although C $K_{4}$ is unstable, NaC$_{2}$ and MgC$_{2}$ are found to be stable. It is shown that NaC$_{2}$ and MgC$_{2}$ are metallic and semi conducting, respectively.

  10. The existence spectrum of Meta(K4(3)>K4(3)-e)

    Institute of Scientific and Technical Information of China (English)

    LO; FARO; Giovanni; TRIPODI; Antoinette

    2010-01-01

    Let H and J1 be both t-uniform hypergraphs. Let J2 be a sub-hypergraph of J1. In this paper, the metamorphosis of a hypergraph decomposition is introduced, denoted by (H, J1 > J2)-design, which is a generalization of the concept of metamorphosis of a graph decomposition. Let Meta(J1>J2) denote the set of all integers v such that there exists a (Kv((3)), J1>J2)-design. We completely determine the set Meta(K4((3))>K4((3))-e).

  11. Historie

    DEFF Research Database (Denmark)

    Poulsen, Jens Aage

    Historie i serien handler om læreplaner og læremidler og deres brug i skolefaget historie. Bogen indeholder nyttige redskaber til at analysere og vurdere læremidler......Historie i serien handler om læreplaner og læremidler og deres brug i skolefaget historie. Bogen indeholder nyttige redskaber til at analysere og vurdere læremidler...

  12. CCM key comparison CCM.D-K4 'Hydrometer'

    Science.gov (United States)

    Lorefice, S.; Becerra, L. O.; Lenard, E.; Lee, Y. J.; Lee, W. G.; Madec, T.; Meury, P. A.; Caceres, J.; Santos, C.; Vamossy, C.; Man, J.; Fen, K.; Toda, K.; Wright, J.; Bettin, H.; Toth, H.

    2016-01-01

    This report presents the comparison philosophy, methodology, analysis and the results of the designed CCM.D-K4 key comparison that covered the calibration of high resolution hydrometers for liquid and alcoholometers in the density range 600 kg/m3 to 2000 kg/m3 at the temperature of 20°C. The main purpose of this comparison was not only to evaluate the degree of equivalence in the calibration of high accuracy hydrometers between NMI participants, but also to link, were it is possible, the results of previous comparisons to Key Comparison Reference Values (KCRVs) of CCM.D-K4. Eleven NMI laboratories took part in the CCM.D-K4 divided in two groups (petals). With the CCM.D-K4 purpose, two similar sets consisting of three hydrometers for liquid density determinations and an alcoholometer were circulated to the NMI participants as a travelling standard in the time interval from January 2011 to April 2012. Twelve Key Comparison Reference Values (KCRVs) for each petal have been obtained at the density values related to the tested density marks of the transfer standards by the results of participants. The KCRVs and corresponding uncertainties were calculated by the weighted mean in the case of consistent results, otherwise the median was used. The degree of equivalence (DoE) with respect to the corresponding KCRV was determined for each participant and, in this particular comparison, the Weighted Least Squares (WLS) method was used to link the individual DoE of each participant by a continuous function. Significant drift of the transfer standards was not detected. This report also gives instructions on calculating pair-wise degrees of equivalence, with the addition of any information on correlations that may be necessary to estimate more accurately as well as the procedure for linking international comparisons to the CCM.D-K4. Finally an example of linkage to the CCM.D-K4 is given by dealing with the results of the bilateral comparison between INRiM and NMIA, which was

  13. Properties of atomic intercalated boron nitride K4 type crystals

    OpenAIRE

    Itoh, Masahiro; Takami, Seiichi; Kawazoe, Yoshiyuki; Adschiri, Tadafumi

    2010-01-01

    The stability of atomic intercalated boron nitride K4 crystal structures, XBN (X=H, Li, Be, B, C, N, O, F, Na, Mg, Al, Si, P, S, Cl, K, Ca, Ga, Ge, As, Se, Br, Rb or Sr) is evaluated by the geometric optimization and frozen phonon calculations based on the first principles calculations. NaBN, MgBN, GaBN, FBN and ClBN are found to be stable. NaBN, GaBN, FBN and ClBN are metallic, whereas MgBN is semiconducting.

  14. Critical Time: Earthquake Response Planning and Schools. [Videotape].

    Science.gov (United States)

    Federal Emergency Management Agency, Washington, DC.

    A videotape that describes what earthquakes are, and examines the disaster planning schools can develop during the first few minutes following an earthquake to assure students and staff survive. The kinds of destruction likely to happen during a damaging earthquake are highlighted. The videotape stresses the need for children and staff to know…

  15. Kindergarten, Here I Come! = Kinder, aqui voy! [Videotape].

    Science.gov (United States)

    2001

    Designed to generate excitement about starting school and to answer the many questions preschool children have about kindergarten, this 18-minute videotape gives preschool children a positive, realistic vision of kindergarten. The videotape, in both English and Spanish versions, is designed to be viewed in preschools or day cares, by parents with…

  16. Tutored Videotape-Instruction in Clinical Decision-Making.

    Science.gov (United States)

    Sox, Harold C.; And Others

    1984-01-01

    A comparison of traditional medical lecturing with a technique using a videotaped lecture that can be interrupted by a tutor for discussion or questions showed similar academic achievement rates but higher student ratings for the videotape method. The technique is recommended for specialized topics or areas in which the supply of expert lecturers…

  17. All about Mammals. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    In this videotape, students learn more about the characteristics of common warm-blooded mammals and what makes them different from other animals. Children also find out how humans are more advanced in structure than other mammals, but how they still share the same basic traits. This videotape correlates to the following National Science Education…

  18. All about Mammals. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    In this videotape, students learn more about the characteristics of common warm-blooded mammals and what makes them different from other animals. Children also find out how humans are more advanced in structure than other mammals, but how they still share the same basic traits. This videotape correlates to the following National Science Education…

  19. Oral History and the Special Library.

    Science.gov (United States)

    Jimenez, Rebecca S.

    1985-01-01

    Discussion of the relationship between oral history and libraries notes the character of oral history (both auditory and visual); types of oral history interviews (focused or subject-oriented, life review); forming an oral history collection (audiotapes, videotapes, transcripts); legal agreements and oral history; and collecting versus creating…

  20. Reading, Writing & Rings: Science Literacy for K-4 Students

    Science.gov (United States)

    McConnell, S.; Spilker, L.; Zimmerman-Brachman, R.

    2007-12-01

    Scientific discovery is the impetus for the K-4 Education program, "Reading, Writing & Rings." This program is unique because its focus is to engage elementary students in reading and writing to strengthen these basic academic skills through scientific content. As science has been increasingly overtaken by the language arts in elementary classrooms, the Cassini Education Program has taken advantage of a new cross-disciplinary approach to use language arts as a vehicle for increasing scientific content in the classroom. By utilizing the planet Saturn and the Cassini-Huygens mission as a model in both primary reading and writing students in these grade levels, young students can explore science material while at the same time learning these basic academic skills. Content includes reading, thinking, and hands-on activities. Developed in partnership with the Cassini-Huygens Education and Public Outreach Program, the Bay Area Writing Project/California Writing Project, Foundations in Reading Through Science & Technology (FIRST), and the Caltech Pre-College Science Initiative (CAPSI), and classroom educators, "Reading, Writing & Rings" blends the excitement of space exploration with reading and writing. All materials are teacher developed, aligned with national science and language education standards, and are available from the Cassini-Huygens website: http://saturn.jpl.nasa.gov/education/edu-k4.cfm Materials are divided into two grade level units. One unit is designed for students in grades 1 and 2 while the other unit focuses on students in grades 3 and 4. Each includes a series of lessons that take students on a path of exploration of Saturn using reading and writing prompts.

  1. Usefulness of Videotape Instruction in an Academic Department of Neurology.

    Science.gov (United States)

    Kaufman, David M.; Kaufman, Rita G.

    1983-01-01

    Videotape instruction produced better performance in identification in only certain areas in a neurology clerkship: neuropsychologic phenomena, disorders with subtle or unique movements, and seizures. The choice and cost of equipment and some professional assurances are discussed. (Author/MLW)

  2. Effects of Videotaped Role Playing on Nurses' Therapeutic Communication Skills

    Science.gov (United States)

    Carpenter, Kay F.; Kroth, Jerome A.

    1976-01-01

    Research determining the effectiveness of videotape recorded (VTR) role playing as a teaching technique was conducted on nurses attending continuing education classes in verbal and nonverbal therapeutic communication skills. VTR appears to be an effective technique. (LH)

  3. Usefulness of Videotape Instruction in an Academic Department of Neurology.

    Science.gov (United States)

    Kaufman, David M.; Kaufman, Rita G.

    1983-01-01

    Videotape instruction produced better performance in identification in only certain areas in a neurology clerkship: neuropsychologic phenomena, disorders with subtle or unique movements, and seizures. The choice and cost of equipment and some professional assurances are discussed. (Author/MLW)

  4. An Evaluation of the Navy’s Health Promotion Videotapes

    Science.gov (United States)

    1990-04-30

    1263. Fisher, L., Rowley, P. T., & Lipkin. M. (1981). Genetic counseling for beta - thalassemia trait following health screening in a health maintenence...studied the impact of a videotaped dental fear-reduction program on moderately and highly fearful people who avoided dental treatment . All subjects who...percentage of subjects who viewed the modeling videotape returned for their test-of-cure examination (an essential part of the treatment ) than those who did

  5. Learning through the lens: ethical considerations in videotaping psychotherapy.

    Science.gov (United States)

    Funkenstein, Amy B; Kessler, Katherine Ann; Schen, Cathy R

    2014-01-01

    Psychiatry training programs have begun to use technology to enhance psychotherapy teaching. Videotaped interviews provide a window into the psychotherapeutic exchange, demystifying the process and capturing verbal and nonverbal interactions, facial expression, and tone of voice-which can illustrate therapeutic elements such as the alliance and resistance. The process of videotaping psychotherapeutic interviews, however, introduces issues related to consent, ethics, and the dynamics of therapy. By examining two cases in which residents asked their patients to videotape a session for didactic purposes and encountered divergent outcomes, we explore the ethical issues unique to providing informed consent for videotaping psychotherapy. Informed consent must be given verbally and in writing. Verbal consent must include discussion of the risks and benefits of therapy. The discussion must also include the risks inherent in memorializing sensitive material on an external device, with disclosure of the logistics of how data will be stored, who will view the recorded material, and when it will be destroyed. Therapists should be aware of the coercive power inherent in the physician-patient relationship and should individualize each informed consent procedure with this knowledge in mind. Despite these potential pitfalls, videotaping sessions provides a wealth of information about both patients and therapists that can improve psychotherapy teaching and supervision, and indirectly improve patient care.

  6. 36 CFR 1280.44 - May I film, photograph, or videotape on NARA property for commercial purposes?

    Science.gov (United States)

    2010-07-01

    ... videotape on NARA property for commercial purposes? 1280.44 Section 1280.44 Parks, Forests, and Public... videotape on NARA property for commercial purposes? No, filming, photographing, and videotaping on NARA property for commercial purposes is prohibited....

  7. Buzz Groups and Videotape Speed Training at Ernst & Ernst

    Science.gov (United States)

    Larick, Donald

    1971-01-01

    Using videotape for short lectures at the end of buzz group problems takes the burden off staff when training 360 auditors in computer techniques. Money conscious Ernst & Ernst puts the cost at $1.90 per lecture, per man. (Journal/EB)

  8. All about Reptiles. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    Dinosaurs may be extinct, but reptiles are distant cousins to the beasts that once walked the earth. From snakes and lizards to iguanas and tuataras, children learn what factors make them different from other animals. In this videotape, students explore the mysterious, often misunderstood, world of reptiles and learn about their characteristics…

  9. The Cutting Edge Technology Report: Designing Smarter Schools. [Videotape].

    Science.gov (United States)

    2000

    This videotape highlights the extent to which school buildings are deteriorating in America and the problems this causes for teaching and learning, describes the Energy Smart School campaign, and details the factors needed in building an Energy Smart School. To build schools that last and to recoup some of the building expense, it suggests…

  10. Factors Influencing Consent to Having Videotaped Mental Health Sessions

    Science.gov (United States)

    Ko, Kenton; Goebert, Deborah

    2011-01-01

    Objective: The authors critically reviewed the literature regarding factors influencing consent to having videotaped mental health sessions. Methods: The authors searched the literature in PubMed, PsycINFO, Google Scholar, and Web of Science from the mid-1950s through February 2009. Results: The authors identified 27 studies, of which 19 (73%)…

  11. Videotaped Presentations by Blind Speakers as Attitudinal Change Agents.

    Science.gov (United States)

    Safran, Stephen P.; Safran, Joan S.

    1986-01-01

    Determined whether a videotaped presentation by a speaker who is blind would more positively influence attitude change and information retention than would a presentation by a sighted speaker. Findings suggested that there were no significant main effects for either presenter or pretest conditions on the measures. (Author/BL)

  12. Use of Videotape Feedback with Severely Disturbed Adolescents.

    Science.gov (United States)

    Michel, Jane; Blitstein, Sheldon

    1979-01-01

    Summarizes the design and effects of a group therapy project using videotape feedback with seriously disturbed adolescents. Offers anecdotal evidence that the feedback facilitated the correction of the participants' distorted body images, low self-esteem, lack of capacity for self-observation, and poor peer relationships. (SS)

  13. Applications of Videotape Resources in Manpower Development Programs. Final Report.

    Science.gov (United States)

    McHugh, Paul

    Mobilization for Youth (MFY) found that videotape had several advantages and uses in a manpower training program. These uses included skills training, interview training, instruction in safety practices, orientation to handling of costly materials, and job analysis. Administrators used it for training employees, for recording consultants' advice,…

  14. All about Animal Life Cycles. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    While watching the development from tadpole to frog, caterpillar to butterfly, and pup to wolf, children learn about the life cycles of animals, the different stages of development, and the average life spans of a variety of creatures. This videotape correlates to the following National Science Education Standards for Life Science: characteristics…

  15. Teaching Techniques for the ESL Literacy Classroom. [Videotape.

    Science.gov (United States)

    Gati, Sally

    This 71-minute videotape presents a three-part program of classroom teaching techniques for teachers of English-as-a-Second-Language (ESL) literacy students. It shows students interacting with ESL literacy teachers, and focuses on: (1) essential teaching tools (chalkboard, easel, overhead projector, butcher paper, realia, pictures, flash cards,…

  16. All about Amphibians. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    This videotape teaches children about their favorite amphibious creatures, as well as amphibians' nearest cousins--toads, newts, and salamanders. Young students discover how these amazing creatures can live both in and out of water, learn about the amphibious life cycle, and compare the differences between amphibians and reptiles. This videotape…

  17. Analysis of a Videotaped Lesson from an Exercise Physiology Perspective

    Science.gov (United States)

    Biren, Greg; Rattigan, Peter

    2006-01-01

    Physical educators can infuse fitness concepts, teach exercise science, and increase activity in their lessons all at the same time. The videotaped lesson is reviewed in terms of its value in developing health- and skill-related fitness, and in relation to the national standards for physical education. (Contains 1 table.)

  18. All about Animal Behavior & Communication. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    Why do animals do what they do? What is the difference between instinct and learned behavior? How do animals communicate? These questions are answered as children examine animal behaviors that help them find food, protect themselves, and care for their young. This videotape correlates to the following National Science Education Standards for Life…

  19. All about Food Chains. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    Whether animals are herbivores, carnivores, or omnivores, each one is part of an eternal food chain that carries on from one generation to the next. In this videotape, students learn more about terms like "predator,""pre-consumer" and "producer," as well as the cycles of food chains and food webs and how they support…

  20. All about Reptiles. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    Dinosaurs may be extinct, but reptiles are distant cousins to the beasts that once walked the earth. From snakes and lizards to iguanas and tuataras, children learn what factors make them different from other animals. In this videotape, students explore the mysterious, often misunderstood, world of reptiles and learn about their characteristics…

  1. All about Dinosaurs. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    Dinosaurs were the rulers of the land 65 million years ago. In this videotape, children learn more about the different kinds of dinosaurs by viewing vivid illustrations and fossil discoveries. Students compare the dinosaurs to their modern kin--snakes, lizards, and crocodiles. Students also listen to different theories to try to answer the big…

  2. Children in America's Schools with Bill Moyers. [Videotape].

    Science.gov (United States)

    Hayden, Jeffrey

    A 2-hour videotape presentation explores the crisis in school building quality in the United States as illustrated by public schools found in Ohio; the challenges dilapidated school facilities impose on child health, safety, and the physical learning environment; and the difference that money makes in providing high-quality educational facilities.…

  3. Tips & Techniques: Storytelling with Puppets and Props. [Videotape].

    Science.gov (United States)

    2002

    On this 20-minute videotape, two accomplished storytellers share their expertise on using puppets and props to enhance the art of storytelling. Schroeder Cherry uses several types of puppets to tell his educational story about the Underground Railroad, while Karen Quinn-Wisniewski entertains her audience of young children with classic fables,…

  4. Mitogen-activated protein kinase kinase 4 (MAP2K4 promotes human prostate cancer metastasis.

    Directory of Open Access Journals (Sweden)

    Janet M Pavese

    Full Text Available Prostate cancer (PCa is the second leading cause of cancer death in the US. Death from PCa primarily results from metastasis. Mitogen-activated protein kinase kinase 4 (MAP2K4 is overexpressed in invasive PCa lesions in humans, and can be inhibited by small molecule therapeutics that demonstrate favorable activity in phase II studies. However, MAP2K4's role in regulating metastatic behavior is controversial and unknown. To investigate, we engineered human PCa cell lines which overexpress either wild type or constitutive active MAP2K4. Orthotopic implantation into mice demonstrated MAP2K4 increases formation of distant metastasis. Constitutive active MAP2K4, though not wild type, increases tumor size and circulating tumor cells in the blood and bone marrow. Complementary in vitro studies establish stable MAP2K4 overexpression promotes cell invasion, but does not affect cell growth or migration. MAP2K4 overexpression increases the expression of heat shock protein 27 (HSP27 protein and protease production, with the largest effect upon matrix metalloproteinase 2 (MMP-2, both in vitro and in mouse tumor samples. Further, MAP2K4-mediated increases in cell invasion are dependent upon heat shock protein 27 (HSP27 and MMP-2, but not upon MAP2K4's immediate downstream targets, p38 MAPK or JNK. We demonstrate that MAP2K4 increases human PCa metastasis, and prolonged over expression induces long term changes in cell signaling pathways leading to independence from p38 MAPK and JNK. These findings provide a mechanistic explanation for human studies linking increases in HSP27 and MMP-2 to progression to metastatic disease. MAP2K4 is validated as an important therapeutic target for inhibiting human PCa metastasis.

  5. Impaired removal of H3K4 methylation affects cell fate determination and gene transcription

    DEFF Research Database (Denmark)

    Lussi, Yvonne C; Mariani, Luca; Rundsten, Carsten Friis;

    2016-01-01

    implicated in development, but how the regulation of H3K4me3 level controls developmental processes is not fully established. Here, we show that the H3K4 demethylase RBR-2, the unique member of the KDM5 family in C. elegans, acts cell-autonomously and in a catalytic-dependent manner to control vulva...

  6. The GluK4 kainate receptor subunit regulates memory, mood, and excitotoxic neurodegeneration.

    Science.gov (United States)

    Lowry, E R; Kruyer, A; Norris, E H; Cederroth, C R; Strickland, S

    2013-04-01

    Though the GluK4 kainate receptor subunit shows limited homology and a restricted expression pattern relative to other kainate receptor subunits, its ablation results in distinct behavioral and molecular phenotypes. GluK4 knockout mice demonstrated impairments in memory acquisition and recall in a Morris water maze test, suggesting a previously unreported role for kainate receptors in spatial memory. GluK4 knockout mice also showed marked hyperactivity and impaired pre-pulse inhibition, thereby mirroring two of the hallmark endophenotypes of patients with schizophrenia and bipolar disorder. Furthermore, we found that GluK4 is a key mediator of excitotoxic neurodegeneration: GluK4 knockout mice showed robust neuroprotection in the CA3 region of the hippocampus following intrahippocampal injection of kainate and widespread neuroprotection throughout the hippocampus following hypoxia-ischemia. Biochemical analysis of kainate- or sham-treated wild-type and GluK4 knockout hippocampal tissue suggests that GluK4 may act through the JNK pathway to regulate the molecular cascades that lead to excitotoxicity. Together, our findings suggest that GluK4 may be relevant to the understanding and treatment of human neuropsychiatric and neurodegenerative disorders.

  7. [Synthesis, spectral analysis and photocatalysis of Ag/K4Nb6,O17 heterojunction catalysts].

    Science.gov (United States)

    Zhang, Feng-li; Cao, Yan-ning; Ying, Song; Chen, Rong; Zhang, Han-hui; Zheng, Qi

    2010-10-01

    K4Nb6O17 photocatalyst was successfully synthesized by low-temperature hydrothermal method with layer structure. Considering that a large number of hydroxyl (Nb-OH) and oxygen species (Nb==O, Nb--O-) exist on the surface of K4Nb6O17 synthesized by hydrothermal method, Ag(en)2+ precursors were employed to synthesize Ag/K4Nb6O17 heterostructure photo-catalysts with highly dispersed Ag. Photocatalytic performance evaluation results show that the photodegradation rate of MO for K4Nb6O17 was remarkably improved when a small amont of Ag was loaded. The best loading dose of Ag is 0.5 at%. Based on various characterizations results of XRD, FTIR, UV-Vis DRS, XRF and TEM, the photocatalytic mechanism of Ag/ K4Nb6O17 heterostructure catalysts was illuminated in detail and the conclusions were drawn as follows: (1) K4Nb6O17 nanocrystals serve as electron and hole sources for degradation of an organic dye; (2) Ag nanoparticles on the surface of K4Nb6O17 nanocrystals act as a sink for the electrons, promote interfacial charge-transfer kinetics between the metal and semiconductor, improve the separation of photogenerated electron-hole pairs, and thus enhance the photocatalytic activity of Ag/K4Nb6O17 photocatalyst.

  8. DVIG ENERGETSKE UČINKOVITOSTI PREMOGOVNEGA KOTLA K4 V TOVARNI VIPAP

    OpenAIRE

    Lekše, Aleš

    2014-01-01

    Diplomsko delo se nanaša na znižanje lastne rabe energije na postrojenju kotla K4 ter na dvig deleža pridobljene energije iz OVE v podjetju Vipap Videm Krško. Na podlagi ugotovitev, da so izvedeni ukrepi znižali lastno rabo električne energije, večji delež OVE v strukturi goriva pa je zagotovil željeno toplotno moč kotla K4, nam zaključni del naloge potrjuje, da so vsi izvedeni ukrepi upravičili ekonomsko in ekološko upravičenost posodobitve postrojenja kotla K4.

  9. On a $\\{K_4,L(Q_3)\\}_{K_3}$-ultrahomogeneous graph

    CERN Document Server

    Dejter, Italo J

    2010-01-01

    A construction based on the Biggs-Smith graph is shown to produce an edge-disjoint union of 102 copies of $K_4$ forming a $\\{K_4,L(Q_3)\\}_{K_3}$-ultrahomogeneous graph as well as the non-line-graphical Menger graph of a self-dual $(102_4)$-configuration. This stands in contrast to and is a follow-up of the self-dual $(42_4)$-configuration of \\cite{D1}, whose Menger graph is a non-line-graphical $\\{K_4,$ $K_{2,2,2}\\}_{K_2}$-ultrahomogeneous graph.

  10. Effects of nickel treatment on H3K4 trimethylation and gene expression.

    Directory of Open Access Journals (Sweden)

    Kam-Meng Tchou-Wong

    Full Text Available Occupational exposure to nickel compounds has been associated with lung and nasal cancers. We have previously shown that exposure of the human lung adenocarcinoma A549 cells to NiCl(2 for 24 hr significantly increased global levels of trimethylated H3K4 (H3K4me3, a transcriptional activating mark that maps to the promoters of transcribed genes. To further understand the potential epigenetic mechanism(s underlying nickel carcinogenesis, we performed genome-wide mapping of H3K4me3 by chromatin immunoprecipitation and direct genome sequencing (ChIP-seq and correlated with transcriptome genome-wide mapping of RNA transcripts by massive parallel sequencing of cDNA (RNA-seq. The effect of NiCl(2 treatment on H3K4me3 peaks within 5,000 bp of transcription start sites (TSSs on a set of genes highly induced by nickel in both A549 cells and human peripheral blood mononuclear cells were analyzed. Nickel exposure increased the level of H3K4 trimethylation in both the promoters and coding regions of several genes including CA9 and NDRG1 that were increased in expression in A549 cells. We have also compared the extent of the H3K4 trimethylation in the absence and presence of formaldehyde crosslinking and observed that crosslinking of chromatin was required to observe H3K4 trimethylation in the coding regions immediately downstream of TSSs of some nickel-induced genes including ADM and IGFBP3. This is the first genome-wide mapping of trimethylated H3K4 in the promoter and coding regions of genes induced after exposure to NiCl(2. This study may provide insights into the epigenetic mechanism(s underlying the carcinogenicity of nickel compounds.

  11. Cellular Membrane Composition Requirement by Antimicrobial and Anticancer Peptide GA-K4.

    Science.gov (United States)

    Mishig-Ochir, Tsogbadrakh; Gombosuren, Davaadulam; Jigjid, Altanchimeg; Tuguldur, Badamkhatan; Chuluunbaatar, Galbadrakh; Urnukhsaikhan, Enerelt; Pathak, Chinar; Lee, Bong-Jin

    2017-01-01

    Naturally occurring antimicrobial peptides important for innate immunity are widely studied for their antimicrobial and anticancer activity. The primary target of these AMPs is believed to be the bacterial cytoplasmic membrane. However, the interaction between cytoplasmic membrane and the antimicrobial peptides remains poorly understood. Therefore to focus on the target membrane composition that is required by AMPs to interact with membranes, we have examined the interaction of the antimicrobial and anticancer active 11-residue GA-K4 (FLKWLFKWAKK) peptide with model and intact cell membranes. Effect on the structural conformational properties of GA-K4 peptide was investigated by means of far-UV CD and fluorescence spectroscopic methods. The different conformation of GA-K4 peptide in large unilamellar vesicles (LUV) bilayer and micelle environment suggest that the curvature has an influence on the secondary structure acquired by the peptide. Furthermore, the leakage experiment result confirmed that GA-K4 induced the leakage of cytoplasmic membrane in Staphylococcus аureus bacterial cells. Fluorescence data revealed the interfacial location of GA-K4 peptide in the model membranes. The blue-shift in emission wavelength by tryptophan residues in fluorescence data indicated the penetration of GA-K4 peptide in micelles and phospholipid bilayers. These results showed that the GA-K4 peptide is a membrane-active peptide and its activity depends on membrane curvature and lipid composition. Although further studies are required to confirm the mechanism of action, the data suggest mechanism of toroidal pore formation for the interaction of GA-K4 peptide with membranes. Our studies will be helpful in better understanding of the membrane requirment of peptides to express their therapeutic effects. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  12. CFP1 Regulates Histone H3K4 Trimethylation and Developmental Potential in Mouse Oocytes

    Directory of Open Access Journals (Sweden)

    Chao Yu

    2017-08-01

    Full Text Available Trimethylation of histone H3 at lysine-4 (H3K4me3 is associated with eukaryotic gene promoters and poises their transcriptional activation during development. To examine the in vivo function of H3K4me3 in the absence of DNA replication, we deleted CXXC finger protein 1 (CFP1, the DNA-binding subunit of the SETD1 histone H3K4 methyltransferase, in developing oocytes. We find that CFP1 is required for H3K4me3 accumulation and the deposition of histone variants onto chromatin during oocyte maturation. Decreased H3K4me3 in oocytes caused global downregulation of transcription activity. Oocytes lacking CFP1 failed to complete maturation and were unable to gain developmental competence after fertilization, due to defects in cytoplasmic lattice formation, meiotic division, and maternal-zygotic transition. Our study highlights the importance of H3K4me3 in continuous histone replacement for transcriptional regulation, chromatin remodeling, and normal developmental progression in a non-replicative system.

  13. Acyclic chromatic indices of K4-minor free graphs%没有K4-图子式的图的无圈边色数

    Institute of Scientific and Technical Information of China (English)

    王维凡; 舒巧君

    2011-01-01

    一个图G的无圈k-边染色是指G的一个正常的不产生双色圈的k-边染色.G的无圈边色数a1(G)定义为使得G有一个无圈k-边染色的最小的整数k.本文完全刻画了最大度不为4的没有K4-图子式的图的无圈边色数.%An acyclic edge coloring of a graph G is a proper edge coloring such that no Dichromatic cycles are produced. The acyclic chromatic index a'(G) of G is the smallest integer k such that G has an acyclic edge coloring using k colors. In this paper, we determine completely the acyclic chromatic indices of K4-minor free graphs with maximum degree not equal to 4.

  14. The Medium and the Message: Oral History, New Media, and a Grassroots History of Working Women

    Science.gov (United States)

    Meyerowitz, Ruth; Zinni, Christine F.

    2009-01-01

    In the Spring of 2000, Ruth Meyerowitz and Christine Zinni began collaborative efforts--inside and outside of academia--to enhance a course on The History of Working Women at SUNY Buffalo. Videotaping the oral histories of women labor leaders, they later teamed up with Michael Frisch and Randforce Associates--a research group at SUNY at Buffalo's…

  15. Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin

    Directory of Open Access Journals (Sweden)

    De Rosa Mario

    2010-05-01

    Full Text Available Abstract Background Chondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as a human anti-inflammatory drug. Among bacteria, Escherichia coli K4 produces a capsule containing a non-sulphate chondroitin and its development may provide an efficient and cheap fermentative production of the polysaccharide. Results A random N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis was performed on E. coli K4 to isolate mutants showing an increased production of chondroitin. Several mutants were isolated, one of which, here named VZ15, produced about 80% more chondroitin than the wild type E. coli. We found that the mutant has a missense mutation in the codon 313 of kfoC, the gene encoding chondroitin polymerase (K4CP, with a change from arginine to glutamine. A docking analysis to explain the increased productivity of the K4CP enzyme is presented. Conclusion The enhanced chondroitin production by the E. coli K4 mutant reported here shows the validity of the strain improvement strategy for more cost-friendly fermentative processes in the production of this pharmaceutically important but so-far expensive polysaccharide.

  16. One of the 10 cells of AA Injection Kicker K4

    CERN Multimedia

    1979-01-01

    The 3.5 GeV/c Antiproton Accumulator (AA) had 2 delay-line type injection kickers, K3 (12 cells) and K4 (10 cells). Here we see one of the K4 cells, with ferrite between stainless-steel plates. Pulse voltage: 61 kV; rise/fall-time 86 ns; flat-top 460 ns; top flatness +-2%. During injection, the open side of the C-shaped kickers was closed off with a fast shutter, so that their stray field would not perturb the stack of already accumulated antiprotons.

  17. Early Book Stages, 0-5 Years [and] Creciendo con Libros (Growing [up] with Books). [Videotape.

    Science.gov (United States)

    Holguin, Roxanna

    Using a lighthearted and simple approach, this 23-minute videotape in English and Spanish versions presents interactions between parents and children while reading books. The children in the videotape range in age from 0 to 5 years. The video is introduced by scenes of children enjoying books while narration discussing the impact of reading to…

  18. I Am Your Child: Health & Nutrition [and] Literacy [and] Safety. [Videotapes].

    Science.gov (United States)

    I Am Your Child Foundation, Beverly Hills, CA.

    Noting the importance of early experiences for the healthy growth and development of children, these three videotapes for parents explore children's health and nutrition, literacy, and safety. Each videotape is 20-25 minutes long. The first video, "Your Healthy Baby," presents information parents need on children's health and nutrition.…

  19. Journey to Planet Earth: The Urban Explosion. The Public Television Series. [Videotape].

    Science.gov (United States)

    1999

    This videotape attempts to show students how the uncontrolled development of the world's major cities has led to a series of problems such as air pollution, water pollution, limited room for waste disposal, housing shortages, and loss of farmland. The videotape profiles four mega-cities: Mexico City, Shanghai, Istanbul, and New York City. Students…

  20. Early Book Stages, 0-5 Years [and] Creciendo con Libros (Growing [up] with Books). [Videotape.

    Science.gov (United States)

    Holguin, Roxanna

    Using a lighthearted and simple approach, this 23-minute videotape in English and Spanish versions presents interactions between parents and children while reading books. The children in the videotape range in age from 0 to 5 years. The video is introduced by scenes of children enjoying books while narration discussing the impact of reading to…

  1. Journey to Planet Earth: The Urban Explosion. The Public Television Series. [Videotape].

    Science.gov (United States)

    1999

    This videotape attempts to show students how the uncontrolled development of the world's major cities has led to a series of problems such as air pollution, water pollution, limited room for waste disposal, housing shortages, and loss of farmland. The videotape profiles four mega-cities: Mexico City, Shanghai, Istanbul, and New York City. Students…

  2. How To Dance through Time. Volume V: Victorian Era Couple Dances. [Videotape].

    Science.gov (United States)

    Teten, Carol

    This 55-minute VHS videotape is the fifth in a series of "How To Dance Through Time" videos. It continues the tradition of the romance of the mid-19th century couple dances, focusing on Victorian era couple dances. The videotape offers 35 variations of the renowned 19th century couple dances, including the waltz, the polka, the galop,…

  3. CTEPP STANDARD OPERATING PROCEDURE FOR VIDEOTAPING CHILD ACTIVITIES (SOP-2.23)

    Science.gov (United States)

    This SOP describes the method for videotaping a preschool child at a home. The CTEPP main study will collect multimedia samples and questionnaire data at the homes of participants (adults and children) during 48-hr sampling periods. Videotaping the activities of 10% of these chi...

  4. Camera Perspective Bias in Videotaped Confessions: Evidence that Visual Attention Is a Mediator

    Science.gov (United States)

    Ware, Lezlee J.; Lassiter, G. Daniel; Patterson, Stephen M.; Ransom, Michael R.

    2008-01-01

    Several experiments have demonstrated a "camera perspective bias" in evaluations of videotaped confessions: videotapes with the camera focused on the suspect lead to judgments of greater voluntariness than alternative presentation formats. The present research investigated potential mediators of this bias. Using eye tracking to measure visual…

  5. H3K4 demethylase activities repress proliferative and postmitotic aging

    Science.gov (United States)

    Alvares, Stacy M; Mayberry, Gaea A; Joyner, Ebony Y; Lakowski, Bernard; Ahmed, Shawn

    2014-01-01

    Homeostasis of postmitotic and proliferating cells is maintained by pathways that repress stress. We found that the Caenorhabditis elegans histone 3 lysine 4 (H3K4) demethylases RBR-2 and SPR-5 promoted postmitotic longevity of stress-resistant daf-2 adults, altered pools of methylated H3K4, and promoted silencing of some daf-2 target genes. In addition, RBR-2 and SPR-5 were required for germ cell immortality at a high temperature. Transgenerational proliferative aging was enhanced for spr-5; rbr-2 double mutants, suggesting that these histone demethylases may function sequentially to promote germ cell immortality by targeting distinct H3K4 methyl marks. RBR-2 did not play a comparable role in the maintenance of quiescent germ cells in dauer larvae, implying that it represses stress that occurs as a consequence of germ cell proliferation, rather than stress that accumulates in nondividing cells. We propose that H3K4 demethylase activities promote the maintenance of chromatin states during stressful growth conditions, thereby repressing postmitotic aging of somatic cells as well as proliferative aging of germ cells. PMID:24134677

  6. Purification of chondroitin precursor from Escherichia coli K4 fermentation broth using membrane processing.

    Science.gov (United States)

    Schiraldi, Chiara; Carcarino, Immacolata Loredana; Alfano, Alberto; Restaino, Odile Francesca; Panariello, Andrea; De Rosa, Mario

    2011-04-01

    Recently the possibility of producing the capsular polysaccharide K4, a fructosylated chondroitin, in fed-batch experiments was assessed. In the present study, a novel downstream process to obtain chondroitin from Escherichia coli K4 fermentation broth was developed. The process is simple, scalable and economical. In particular, downstream procedures were optimized with a particular aim of purifying a product suitable for further chemical modifications, in an attempt to develop a biotechnological platform for chondroitin sulfate production. During process development, membrane devices (ultrafiltration/diafiltration) were exploited, selecting the right cassette cut-offs for different phases of purification. The operational conditions (cross-flow rate and transmembrane pressure) used for the process were determined on an ÄKTA cross-flow instrument (GE Healthcare, USA), a lab-scale automatic tangential flow filtration system. In addition, parameters such as selectivity and throughput were calculated based on the analytical quantification of K4 and defructosylated K4, as well as the major contaminants. The complete downstream procedure yielded about 75% chondroitin with a purity higher than 90%.

  7. Research-Based Methods of Reading Instruction for English Language Learners, Grades K-4

    Science.gov (United States)

    Vaughn, Sharon; Linan-Thompson, Sylvia

    2007-01-01

    At last, a truly helpful, practical guide for K-4 teachers who want to ensure that their approach to teaching young English language learners is based on research and grounded in proven classroom practices. The authors of ASCD's best-selling book "Research-Based Methods of Reading Instruction, Grades K-3" explain how you can help the English…

  8. Research-Based Methods of Reading Instruction for English Language Learners, Grades K-4

    Science.gov (United States)

    Vaughn, Sharon; Linan-Thompson, Sylvia

    2007-01-01

    At last, a truly helpful, practical guide for K-4 teachers who want to ensure that their approach to teaching young English language learners is based on research and grounded in proven classroom practices. The authors of ASCD's best-selling book "Research-Based Methods of Reading Instruction, Grades K-3" explain how you can help the English…

  9. From Inception to Reflection: Ohio's K-4 Content-Enriched Mandarin Chinese Curriculum

    Science.gov (United States)

    Robinson, Deborah W.

    2009-01-01

    In 2006 the Ohio Department of Education (ODE) submitted and received a three-year Foreign Language Assistance Program grant from the U.S. Department of Education to write and pilot a K-4 content-enriched Mandarin curriculum and to build online professional development modules to support the curriculum. Once funded, ODE formed an advisory…

  10. Minimal (C4,K4;7)-P-graphs%极小(C4,K4;7)-平面图

    Institute of Scientific and Technical Information of China (English)

    段春燕

    2014-01-01

    An (H1 ,H2;n)-P-graph is a graph with order n,which does not contain a subgraph isomorphic to H1 ,and its complement has no subgraph isomorphic to H2 .G is a minimal (H1 ,H2;n)-P-graph,if G is an (H1 ,H2;n)-P-graph and G′obtained by deleting any one edge from G is not an (H1 ,H2;n)-P-graph.In this paper,the minimal (C4 ,K4;7 )-P-graphs are given.%若平面图G没有与H1同构的子图,且G珔没有与H2同构的子图,则G称为(H1,H2)-平面图。(H1,H2;n)-平面图表示n阶的(H1,H2)-平面图。若G是(H1,H2;n)-平面图,去掉G的任意一条边,都不再是(H1,H2;n)-平面图,则G称为极小(H1,H2;n)-平面图。本文给出了所有的极小(C4,K4;7)-平面图,有助于证明PR(C4,K8)。

  11. Electrochemical Catalysis of Inorganic Complex K4[Fe(CN)6] by Shewanella oneidensis MR-1

    DEFF Research Database (Denmark)

    Zheng, Zhiyong; Wu, Ranran; Xiao, Yong

    to the redox proteins localized to the outer-membrane, for example, the MtrC, MtrB,MtrA and CymA2. Here we investigate its electrochemical properties towards redox inorganic redox compounds. It shows strong electrocatalysis toward electrochemical oxidation of K4[Fe(CN)6]. As a redox molecule, K4[Fe(CN)6] gives...... on the selectivity and electrocatalysis mechanisms of Shewanella oneidensis MR-1 are under investigation. The ability of Shewanella oneidensis MR-1 to catalyze redox action of inorganic metal complex compounds will provide an insight on metal cycles in nature....... disappearance of the cathodic peak and strengthen of the anodic peak, which is a typical catalysis feature of electrochemical oxidation. Further experiments show that Shewanella oneidensis MR-1 does not give such electrocatalysis to redox compounds such as Ru[(NH3)6]Cl3 and Resorufin. Systematic study...

  12. Timing of transcriptional quiescence during gametogenesis is controlled by global histone H3K4 demethylation.

    Science.gov (United States)

    Xu, Mengshu; Soloveychik, Maria; Ranger, Mathieu; Schertzberg, Michael; Shah, Zarna; Raisner, Ryan; Venkatasubrahmanyan, Shivkumar; Tsui, Kyle; Gebbia, Marinella; Hughes, Tim; van Bakel, Harm; Nislow, Corey; Madhani, Hiten D; Meneghini, Marc D

    2012-11-13

    Gametes are among the most highly specialized cells produced during development. Although gametogenesis culminates in transcriptional quiescence in plants and animals, regulatory mechanisms controlling this are unknown. Here, we confirm that gamete differentiation in the single-celled yeast Saccharomyces cerevisiae is accompanied by global transcriptional shutoff following the completion of meiosis. We show that Jhd2, a highly conserved JARID1-family histone H3K4 demethylase, activates protein-coding gene transcription in opposition to this programmed transcriptional shutoff, sustaining the period of productive transcription during spore differentiation. Moreover, using genome-wide nucleosome, H3K4me, and transcript mapping experiments, we demonstrate that JHD2 globally represses intergenic noncoding transcription during this period. The widespread transcriptional defects of JHD2 mutants are associated with precocious differentiation and the production of stress-sensitive spores, demonstrating that Jhd2 regulation of the global postmeiotic transcriptional program is critical for the production of healthy meiotic progeny.

  13. 不再当大男孩儿 KIA K4

    Institute of Scientific and Technical Information of China (English)

    张伟平

    2014-01-01

    东风悦达·起亚K系家族给我留下的印象,就好比是一群大男孩儿,各个都拥有一副帅气的容貌且充满活力。不过从今天开始,K系家族出现了一名"另类",一款相对成熟、稳重的车型——K4!

  14. Supplement to CCM.D-K4 'Hydrometer' report: linkage of EURAMET.M.D-K4 comparison, SIM.M.D-K4 comparison and the supplementary SIM.M.D-S2 to CCM.D-K4 'Hydrometer'

    Science.gov (United States)

    Lorefice, S.; Becerra, L. O.

    2017-01-01

    Evaluation of different types of comparisons to a common set of reference values of a CIPM key comparison is essential to satisfy the concept of the CIPM Mutual Recognition Arrangement (CIPM MRA), where the DoEs of any participant who took part in comparisons should be within the Calibration and Measurement Capability (CMC) section of the CIPM MRA Key Comparison Data Base. The subject of this supplementary report is therefore to present the equivalence of each National Metrological Institute (NMI) participant in the CCM.D-K4 'Hydrometer' key comparison, which was performed in the density range 600 kg/m3 to 2000 kg/m3 at the temperature of 20 °C, and the linkage of the European and Inter-American NMI results performed in the RMO.M.D-K4 comparisons as well as those of the supplementary SIM.M.D-S2 to the common set of KCRVs of the CCM.D-K4 'Hydrometer'. The linking procedure has been obtained by numerical simulation, based on the Monte Carlo method, in which the differences in the results of the different comparison between the intended laboratory and one or more linking laboratory/ies, which took part in both comparisons, are correlated with a continuous function describing the DoEs of the linking laboratory/ies with respect to the common set of KCRVs of the CCM.D-K4. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  15. Drosophila KDM2 is a H3K4me3 demethylase regulating nucleolar organization

    Directory of Open Access Journals (Sweden)

    Birchler James A

    2009-10-01

    Full Text Available Abstract Background CG11033 (dKDM2 is the Drosophila homolog of the gene KDM2B. dKDM2 has been known to possess histone lysine demethylase activity towards H3K36me2 in cell lines and it regulates H2A ubiquitination. The human homolog of the gene has dual activity towards H3K36me2 as well as H3K4me3, and plays an important role in cellular senescence. Findings We have used transgenic flies bearing an RNAi construct for the dKDM2 gene. The knockdown of dKDM2 gene was performed by crossing UAS-RNAi-dKDM2 flies with actin-Gal4 flies. Western blots of acid extracted histones and immunofluoresence analysis of polytene chromosome showed the activity of the enzyme dKDM2 to be specific for H3K4me3 in adult flies. Immunofluoresence analysis of polytene chromosome also revealed the presence of multiple nucleoli in RNAi knockdown mutants of dKDM2 and decreased H3-acetylation marks associated with active transcription. Conclusion Our findings indicate that dKDM2 is a histone lysine demethylase with specificity for H3K4me3 and regulates nucleolar organization.

  16. Inducible Deletion of Protein Kinase Map4k4 in Obese Mice Improves Insulin Sensitivity in Liver and Adipose Tissues.

    Science.gov (United States)

    Danai, Laura V; Flach, Rachel J Roth; Virbasius, Joseph V; Menendez, Lorena Garcia; Jung, Dae Young; Kim, Jong Hun; Kim, Jason K; Czech, Michael P

    2015-07-01

    Studies in vitro suggest that mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4) attenuates insulin signaling, but confirmation in vivo is lacking since Map4k4 knockout is lethal during embryogenesis. We thus generated mice with floxed Map4k4 alleles and a tamoxifen-inducible Cre/ERT2 recombinase under the control of the ubiquitin C promoter to induce whole-body Map4k4 deletion after these animals reached maturity. Tamoxifen administration to these mice induced Map4k4 deletion in all tissues examined, causing decreased fasting blood glucose concentrations and enhanced insulin signaling to AKT in adipose tissue and liver but not in skeletal muscle. Surprisingly, however, mice generated with a conditional Map4k4 deletion in adiponectin-positive adipocytes or in albumin-positive hepatocytes displayed no detectable metabolic phenotypes. Instead, mice with Map4k4 deleted in Myf5-positive tissues, including all skeletal muscles tested, were protected from obesity-induced glucose intolerance and insulin resistance. Remarkably, these mice also showed increased insulin sensitivity in adipose tissue but not skeletal muscle, similar to the metabolic phenotypes observed in inducible whole-body knockout mice. Taken together, these results indicate that (i) Map4k4 controls a pathway in Myf5-positive cells that suppresses whole-body insulin sensitivity and (ii) Map4k4 is a potential therapeutic target for improving glucose tolerance and insulin sensitivity in type 2 diabetes.

  17. Video voyeurs and the covert videotaping of unsuspecting victims: psychological and legal consequences.

    Science.gov (United States)

    Simon, R I

    1997-09-01

    Video voyeurs employ state of the art technology to gain access into the most private places where victims are covertly videotaped. Women are the usual victims of video voyeurs as they change their clothes, perform natural functions or engage in sexual activities. When the videotaping is discovered by the victim, serious psychological harm may result. A civil suit is the most common legal remedy sought. Criminal sanctions, when available, are often insufficient compared to the seriousness of the crime. While unauthorized, covert audiotaping is forbidden by both federal and state codes, videotaping is often not specifically mentioned. It appears that legislators do not fully appreciate the burgeoning of covert videotaping, the technological advances that have greatly expanded the possibilities for voyeuristic viewing and the harm done to victims of video voyeurs. Appropriate criminal sanctions need to be included in privacy statutes for unauthorized, video surveillance with or without accompanying audio transcription.

  18. Genomic distribution of CHD7 on chromatin tracks H3K4 methylation patterns.

    Science.gov (United States)

    Schnetz, Michael P; Bartels, Cynthia F; Shastri, Kuntal; Balasubramanian, Dheepa; Zentner, Gabriel E; Balaji, Ravishankar; Zhang, Xiaodong; Song, Lingyun; Wang, Zhenghe; Laframboise, Thomas; Crawford, Gregory E; Scacheri, Peter C

    2009-04-01

    CHD7 is a member of the chromodomain helicase DNA binding domain family of ATP-dependent chromatin remodeling enzymes. De novo mutation of the CHD7 gene is a major cause of CHARGE syndrome, a genetic disease characterized by a complex constellation of birth defects (Coloboma of the eye, Heart defects, Atresia of the choanae, severe Retardation of growth and development, Genital abnormalities, and Ear abnormalities). To gain insight into the function of CHD7, we mapped the distribution of the CHD7 protein on chromatin using the approach of chromatin immunoprecipitation on tiled microarrays (ChIP-chip). These studies were performed in human colorectal carcinoma cells, human neuroblastoma cells, and mouse embryonic stem (ES) cells before and after differentiation into neural precursor cells. The results indicate that CHD7 localizes to discrete locations along chromatin that are specific to each cell type, and that the cell-specific binding of CHD7 correlates with a subset of histone H3 methylated at lysine 4 (H3K4me). The CHD7 sites change concomitantly with H3K4me patterns during ES cell differentiation, suggesting that H3K4me is part of the epigenetic signature that defines lineage-specific association of CHD7 with specific sites on chromatin. Furthermore, the CHD7 sites are predominantly located distal to transcription start sites, most often contained within DNase hypersensitive sites, frequently conserved, and near genes expressed at relatively high levels. These features are similar to those of gene enhancer elements, raising the possibility that CHD7 functions in enhancer mediated transcription, and that the congenital anomalies in CHARGE syndrome are due to alterations in transcription of tissue-specific genes normally regulated by CHD7 during development.

  19. The Role of GluK4 in Synaptic Plasticity and Affective Behavior in Mice

    Science.gov (United States)

    Catches, Justin Samuel

    Kainate receptors (KARs) are glutamate-gated ion channels that signal through both ionotropic and metabotropic pathways (Contractor et al., 2011). Combinations of five KAR subunits (GluK1-5) form tetrameric receptors with GluK1, GluK2, and GluK3 able to form functional homomeric channels. The high-affinity subunits, GluK4 and GluK5, do not form homomeric channels but modify the properties of heteromeric receptors. Expression of the GluK4 receptor subunit in the forebrain is restricted to the CA3 region of the hippocampus and dentate gyrus regions where KARs modulate synaptic plasticity. In this study, ablation of Grik4, which encodes GluK4, in mice reduced KAR synaptic currents and altered activation properties of postsynaptic receptors but left two forms of presynaptic short-term plasticity intact. Disruption of both Grik4 and Grik5 caused complete loss of the postsynaptic ionotropic KAR current and impaired presynaptic frequency facilitation. Additionally, KAR surface expression was altered at pre- and postsynaptic sites at the MF synapse. Despite the loss of ionotropic signaling, KAR-mediated inhibition of the slow afterhyperpolarization current, which is dependent on metabotropic signaling, was intact in CA3 neurons. Long-term potentiation at the MF-CA3 synapse was reduced, likely through a loss of KAR modulation of excitability of the presynaptic MF axons. Genetic variants in the human GRIK4 gene alter the susceptibility for affective disorders (Bloss and Hunter, 2010). We found that ablation of Grik4 in mice resulted in reduced anxiety and an antidepressant-like phenotype. In the elevated zero-maze, a test for anxiety and risk taking behavior, and in two anxiogenic tests, marble-burying and novelty-induced suppression of feeding, anxiety-like behavior was consistently reduced in knockout animals. In the forced swim, a test of learned helplessness used to determine depression-like behavior, knockout mice demonstrated significantly less immobility suggesting

  20. Synthesis and characterization of the ternary telluroargentate K4[Ag18Te11

    KAUST Repository

    Davaasuren, Bambar

    2014-10-19

    The ternary potassium telluroargentate(I), K4[Ag18Te11], was prepared by solvothermal synthesis in ethylenediamine at 160 °C. It crystallizes in the cubic space group Fm3¯ m (no. 225) with the cell parameter a = 18.6589(6) Å. The crystal structure can be described as a [Ag18Te11]4- three-dimensional anionic framework with the voids accommodating potassium cations. Chemical bonding analysis reveals polar covalent Ag-Te bonds and considerable Ag-Ag interactions, which support the complex anionic character of the structure. The compound is thermally stable up to 450 °C in an inert atmosphere.

  1. Structural insight into the recognition of the H3K4me3 mark by the TFIID subunit TAF3

    NARCIS (Netherlands)

    van Ingen, H.|info:eu-repo/dai/nl/297054651; van Schaik, F.M.A.; Wienk, H.|info:eu-repo/dai/nl/203884884; Ballering, J.|info:eu-repo/dai/nl/325784876; Rehmann, H.; Dechesne, A.C.|info:eu-repo/dai/nl/304841684; Kruijzer, J.A.W.; Liskamp, R.M.J.|info:eu-repo/dai/nl/069091315; Timmers, H.T.M.; Boelens, R.|info:eu-repo/dai/nl/070151407

    2008-01-01

    Trimethylation of lysine residue K4 of histone H3 (H3K4me3) strongly correlates with active promoters for RNA polymerase II-transcribed genes. Several reader proteins, including the basal transcription factor TFIID, for this nucleosomal mark have been identified. Its TAF3 subunit specifically binds

  2. Electrochemical Catalysis of Inorganic Complex K4[Fe(CN)6] by Shewanellaoneidensis MR-1

    DEFF Research Database (Denmark)

    Zheng, Zhiyong; Wu, Ranran; Xiao, Yong

    The interaction between metal and bacteria is a universal and important biogeochemical processin environment. As a dissimilatory metal reduction bacterium, the electrochemically activebacteriium Shewanella oneidensis MR-1 can transfer intracellular electrons to minerals. This ability is attribute...... andelectrocatalysis mechanisms of S. oneidensis MR-1 are under investigation. The ability of S. oneidensis MR-1 to catalyze redoxaction of inorganic metal complex compounds will provide an insight on metal cycles in nature....... to the redox proteins localized on the outer-membrane, for example, the MtrC, MtrB, MtrA and CymA2. Here we investigate its electrochemical properties towards redoxinorganic redox compounds. It shows strong electrocatalysis toward electrochemical oxidation of K4[Fe(CN)6]. As a redox molecule, K4[Fe(CN)6] gives......, with almost disappearance of the cathodic peak and strengthen of the anodic peak, which is a typical catalysis feature of electrochemical oxidation. Further experiments show that S. oneidensis MR-1 does not give such electrocatalysis toredox compounds such as Ru[(NH3)6]Cl3and Resorufin. Selectivity...

  3. Factors affecting early elementary (K--4) teachers' introduction of the nature of science: A national survey

    Science.gov (United States)

    Sweeney, Sophia Jean

    A researcher-developed questionnaire regarding the importance and developmental appropriateness of 12 specific elements of the nature of science (Alshamrani, 2008) for early elementary (kindergarten through fourth grade [K-4]) science instruction was mailed to a random sample of U.S. K-4 teachers. At least half (N = 377) of the respondents reported introducing the inferential, empirical, creative, collaborative, tentative, and cultural aspects of the nature of science (NOS) during the current school year, as well as the idea that no single step-by-step scientific method exists. Over 90% of respondents identified the inferential, empirical, and creative aspects of the NOS as developmentally appropriate for the grade level taught. Based on a 5 point scale (0=not at all important and 4 =very important), the mean scores of eight NOS elements were above a value of three, which corresponded with the descriptor somewhat important to include in K-4 science instruction: the inferential, empirical, creative, collaborative, cultural, and tentative NOS, along with the ideas that replication is an important aspect of experimental research and that no single stepwise scientific method exists. A series of binary logistic regression analyses were used to explore the impact of three predictor variables (developmental appropriateness, importance, and presence in state standards) on teachers' self-reported introduction of each of the NOS elements during the 2009-2010 school year. The data for the presence of the NOS elements in state standards were extracted from a previous study (McComas, Lee, & Sweeney, 2009). Developmental appropriateness was a significant predictor of teachers' introduction of the NOS element for all except the collaborative, empirical and inferential aspects of NOS. Importance was a significant predictor for all 12 NOS elements of interest. A NOS element's presence in the state standards significantly predicted teachers' introduction of that NOS element for only

  4. Analysis of the Mitogen-activated protein kinase kinase 4 (MAP2K4 tumor suppressor gene in ovarian cancer

    Directory of Open Access Journals (Sweden)

    Ryland Georgina L

    2011-05-01

    Full Text Available Abstract Background MAP2K4 is a putative tumor and metastasis suppressor gene frequently found to be deleted in various cancer types. We aimed to conduct a comprehensive analysis of this gene to assess its involvement in ovarian cancer. Methods We screened for mutations in MAP2K4 using High Resolution Melt analysis of 149 primary ovarian tumors and methylation at the promoter using Methylation-Specific Single-Stranded Conformation Polymorphism analysis of 39 tumors. We also considered the clinical impact of changes in MAP2K4 using publicly available expression and copy number array data. Finally, we used siRNA to measure the effect of reducing MAP2K4 expression in cell lines. Results In addition to 4 previously detected homozygous deletions, we identified a homozygous 16 bp truncating deletion and a heterozygous 4 bp deletion, each in one ovarian tumor. No promoter methylation was detected. The frequency of MAP2K4 homozygous inactivation was 5.6% overall, and 9.8% in high-grade serous cases. Hemizygous deletion of MAP2K4 was observed in 38% of samples. There were significant correlations of copy number and expression in three microarray data sets. There was a significant correlation between MAP2K4 expression and overall survival in one expression array data set, but this was not confirmed in an independent set. Treatment of JAM and HOSE6.3 cell lines with MAP2K4 siRNA showed some reduction in proliferation. Conclusions MAP2K4 is targeted by genetic inactivation in ovarian cancer and restricted to high grade serous and endometrioid carcinomas in our cohort.

  5. 关于多重多部图λKn(t)的(K4-e,λ)—分解%Decomposition of Complete Multipartite Multi-graph λKn(t) into K4-e

    Institute of Scientific and Technical Information of China (English)

    赵彤

    2001-01-01

    Hoffman在文献[6]中已经完全解决了多重完全图λKn(t)的(K4-e,λ)一分解问题.本文将Hoffman的结果从多重完全图推广到多重多部图,证明了λKn(t)的(K4-e,λ)-分解存在的充分必要条件.

  6. Inhibition of H3K4me2 Demethylation Protects Auditory Hair Cells from Neomycin-Induced Apoptosis.

    Science.gov (United States)

    He, Yingzi; Yu, Huiqian; Cai, Chengfu; Sun, Shan; Chai, Renjie; Li, Huawei

    2015-08-01

    Aminoglycoside-induced hair cell loss is a major cause of hearing impairment in children and deserves more attention in medical research. Epigenetic mechanisms have been shown to protect hair cells from ototoxic drugs. In this study, we focused on the role of dimethylated histone H3K4 (H3K4me2) in hair cell survival. To investigate the effects of lysine-specific demethylase 1 (LSD1)--the histone demethylase primarily responsible for demethylating H3K4me2--on neomycin-induced hair cell loss, isolated cochleae were pretreated with LSD1 inhibitors followed by neomycin exposure. There was a severe loss of hair cells in the organ of Corti after neomycin exposure, and inhibition of LSD1 significantly protected against neomycin-induced hair cell loss. H3K4me2 expression in the nuclei of hair cells decreased after exposure to neomycin, and blocking the decreased expression of H3K4me2 with LSD1 inhibitors prevented hair cell loss. Local delivery of these inhibitors in vivo also protected hair cells from neomycin-induced ototoxicity and maintained the hearing threshold in mice as determined by auditory brain stem response. This inhibition of neomycin-induced apoptosis occurs via reduced caspase-3 activation. Together, our findings demonstrate the protective role for H3K4me2 against neomycin-induced hair cell loss and hearing loss.

  7. Use of home videotapes to confirm parental reports of regression in autism.

    Science.gov (United States)

    Goldberg, Wendy A; Thorsen, Kara L; Osann, Kathryn; Spence, M Anne

    2008-07-01

    The current study examined consistency between parental reports on early language development and behaviors in non-language domains and observer-coded videotapes of young children with and without autism spectrum disorder (ASD) and autistic regression. Data are reported on 56 children (84% male) with ASD (early onset or autistic regression) and 14 typically developing children (57% male) who had home videotapes. Unique to the current study is the independent identification of loss/no loss for each child by both parental report and observer-coded home videotapes and the examination of agreement between these two methods. Results indicate substantial concordance between parental report and observer codes for onset and loss of expressive language, but minimal concordance for loss in non-language domains, suggesting a need for supplementation of parental reports in these areas.

  8. Water in Comet 2/2003 K4 (LINEAR) with Spitzer

    CERN Document Server

    Woodward, Charles E; Bockelee-Morvan, Dominique; Gehrz, R D

    2007-01-01

    We present sensitive 5.5 to 7.6 micron spectra of comet C/2003 K4 (LINEAR) obtained on 16 July 2004 (r_{h} = 1.760 AU, Delta_{Spitzer} = 1.409 AU, phase angle 35.4 degrees) with the Spitzer Space Telescope. The nu_{2} vibrational band of water is detected with a high signal-to-noise ratio (> 50). Model fitting to the best spectrum yields a water ortho-to-para ratio of 2.47 +/- 0.27, which corresponds to a spin temperature of 28.5^{+6.5}_{-3.5} K. Spectra acquired at different offset positions show that the rotational temperature decreases with increasing distance from the nucleus, which is consistent with evolution from thermal to fluorescence equilibrium. The inferred water production rate is (2.43 +/- 0.25) \\times 10^{29} molec. s^{-1}. The spectra do not show any evidence for emission from PAHs and carbonate minerals, in contrast to results reported for comets 9P/Tempel 1 and C/1995 O1 (Hale-Bopp). However, residual emission is observed near 7.3 micron the origin of which remains unidentified.

  9. Improved immunoelectron microscopic method for localizing cytoskeletal proteins in Lowicryl K4M embedded tissues.

    Science.gov (United States)

    Loesser, K E; Doane, K J; Wilson, F J; Roisen, F J; Malamed, S

    1986-11-01

    We have modified the Lowicryl K4M low-temperature dehydration and embedding procedure for immunoelectron microscopy to provide improved ultrastructural detail and facilitate the localization of actin and tubulin in isolated rat adrenocortical cells, chick spinal cord with attached dorsal root ganglia (SC-DRG), and cultured dorsal root ganglia (DRG). Cells and tissues were fixed for immunocytochemistry either in a mixture of 2% paraformaldehyde and 0.25% glutaraldehyde (0.1 M PIPES buffer, pH 7.3) or in a mixture of 0.3% glutaraldehyde and 1.0% ethyldimethylaminopropylcarbodiimide (0.1 M phosphate buffered saline, pH 7.3). Dehydration was in ethanol at progressively lower temperatures to -35 degrees C. Infiltration at -35 degrees C was followed by ultraviolet polymerization at -20 degrees C. Comparable samples were fixed in glutaraldehyde and osmium tetroxide and embedded in Epon 812 or Epon-Araldite. Post-embedding immunostaining of thin sections utilized commercially available monoclonal antibodies to tubulin and actin followed by the protein A-gold technique (Roth et al., Endocrinology 108:247, 1981). Actin immunoreactivity was observed at the periphery of mitochondria and between mitochondria and lipid droplets in rat adrenocortical cells and at the periphery of neuronal cell processes of SC-DRG. Tubulin immunoreactivity was associated with microtubules throughout neurites of cultured DRG. Our modified technique allows preservation of ultrastructural details as well as localization of antigens by immunoelectron microscopy.

  10. The Role of H3K4me3 in Transcriptional Regulation Is Altered in Huntington's Disease.

    Directory of Open Access Journals (Sweden)

    Xianjun Dong

    Full Text Available Huntington's disease (HD is an autosomal-dominant neurodegenerative disorder resulting from expansion of CAG repeats in the Huntingtin (HTT gene. Previous studies have shown mutant HTT can alter expression of genes associated with dysregulated epigenetic modifications. One of the most widely studied chromatin modifications is trimethylated lysine 4 of histone 3 (H3K4me3. Here, we conducted the first comprehensive study of H3K4me3 ChIP-sequencing in neuronal chromatin from the prefrontal cortex of six HD cases and six non-neurologic controls, and its association with gene expression measured by RNA-sequencing. We detected 2,830 differentially enriched H3K4me3 peaks between HD and controls, with 55% of them down-regulated in HD. Although H3K4me3 signals are expected to be associated with mRNA levels, we found an unexpected discordance between altered H3K4me3 peaks and mRNA levels. Gene ontology (GO term enrichment analysis of the genes with differential H3K4me3 peaks, revealed statistically significantly enriched GO terms only in the genes with down-regulated signals in HD. The most frequently implicated biological process terms are organ morphogenesis and positive regulation of gene expression. More than 9,000 H3K4me3 peaks were located not near any recognized transcription start sites and approximately 36% of these "distal" peaks co-localized to known enhancer sites. Six transcription factors and chromatin remodelers are differentially enriched in HD H3K4me3 distal peaks, including EZH2 and SUZ12, two core subunits of the polycomb repressive complex 2 (PRC2. Moreover, PRC2 repressive state was significantly depleted in HD-enriched peaks, suggesting the epigenetic role of PRC2 inhibition associated with up-regulated H3K4me3 in Huntington's disease. In summary, our study provides new insights into transcriptional dysregulation of Huntington's disease by analyzing the differentiation of H3K4me3 enrichment.

  11. Two Automated Mechanisms to Create Electures and to Videotape Regular Lectures

    Directory of Open Access Journals (Sweden)

    Nael Hirzallah

    2012-01-01

    Full Text Available Many institutes are offering lectures that students may watch online. These lectures vary in type from true multimedia sophisticated documents to eLectures to videotaped regular lectures that may include auto generated closed caption. This paper studies some formats that can be easily composed by instructors. Whether adopting the eLectures format or videotaped lecture format, the paper proposes two automated mechanisms to create such lectures without the intervention of a camera or video mixer operator. The first mechanism depends on the absence of mouse motion events; while the second mechanism depends on the instructors facial and hand detections.

  12. Comparative analyses of H3K4 and H3K27 trimethylations between the mouse cerebrum and testis.

    Science.gov (United States)

    Cui, Peng; Liu, Wanfei; Zhao, Yuhui; Lin, Qiang; Zhang, Daoyong; Ding, Feng; Xin, Chengqi; Zhang, Zhang; Song, Shuhui; Sun, Fanglin; Yu, Jun; Hu, Songnian

    2012-04-01

    The global features of H3K4 and H3K27 trimethylations (H3K4me3 and H3K27me3) have been well studied in recent years, but most of these studies were performed in mammalian cell lines. In this work, we generated the genome-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChIP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLiD technology. We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression, function and development. Moreover, we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes, which contradicts the findings in previous studies. Finally, we observed that bivalent domains, with both H3K4me3 and H3K27me3, existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes. However, the bivalent domains tend towards a "winner-takes-all" approach to regulate the expression of associated genes. We also verified the above results in mouse ES cells. As expected, the results in ES cells are consistent with those in cerebrum and testis. In conclusion, we present two very important findings. One is that H3K4me3 and H3K27me3 rarely occur in silent genes. The other is that bivalent domains may adopt a "winner-takes-all" principle to regulate gene expression. Copyright © 2012 Beijing Institute of Genomics, Chinese Academy of Sciences. Published by Elsevier Ltd. All rights reserved.

  13. Predicting the probability of H3K4me3 occupation at a base pair from the genome sequence context.

    Science.gov (United States)

    Ha, Misook; Hong, Soondo; Li, Wen-Hsiung

    2013-05-01

    Histone modifications regulate chromatin structure and gene expression. Although nucleosome formation is known to be affected by primary DNA sequence composition, no sequence signature has been identified for histone modifications. It is known that dense H3K4me3 nucleosome sites are accompanied by a low density of other nucleosomes and are associated with gene activation. This observation suggests a different sequence composition of H3K4me3 from other nucleosomes. To understand the relationship between genome sequence and chromatin structure, we studied DNA sequences at histone modification sites in various human cell types. We found sequence specificity for H3K4me3, but not for other histone modifications. Using the sequence specificities of H3 and H3K4me3 nucleosomes, we developed a model that computes the probability of H3K4me3 occupation at each base pair from the genome sequence context. A comparison of our predictions with experimental data suggests a high performance of our method, revealing a strong association between H3K4me3 and specific genomic DNA context. The high probability of H3K4me3 occupation occurs at transcription start and termination sites, exon boundaries and binding sites of transcription regulators involved in chromatin modification activities, including histone acetylases and enhancer- and insulator-associated factors. Thus, the human genome sequence contains signatures for chromatin modifications essential for gene regulation and development. Our method may be applied to find new sequence elements functioning by chromatin modulation. Software and supplementary data are available at Bioinformatics online.

  14. Comparative Analyses of H3K4 and H3K27 Trimethylations Between the Mouse Cerebrum and Testis

    KAUST Repository

    Cui, Peng

    2012-06-08

    The global features of H3K4 and H3K27 trimethylations (H3K4me3 and H3K27me3) have been well studied in recent years, but most of these studies were performed in mammalian cell lines. In this work, we generated the genome-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChIP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLiD technology. We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression, function and development. Moreover, we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes, which contradicts the findings in previous studies. Finally, we observed that bivalent domains, with both H3K4me3 and H3K27me3, existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes. However, the bivalent domains tend towards a “winner-takes-all” approach to regulate the expression of associated genes. We also verified the above results in mouse ES cells. As expected, the results in ES cells are consistent with those in cerebrum and testis. In conclusion, we present two very important findings. One is that H3K4me3 and H3K27me3 rarely occur in silent genes. The other is that bivalent domains may adopt a “winner-takes-all” principle to regulate gene expression.

  15. Predicting the probability of H3K4me3 occupation at a base pair from the genome sequence context

    Science.gov (United States)

    Ha, Misook; Hong, Soondo; Li, Wen-Hsiung

    2013-01-01

    Motivation: Histone modifications regulate chromatin structure and gene expression. Although nucleosome formation is known to be affected by primary DNA sequence composition, no sequence signature has been identified for histone modifications. It is known that dense H3K4me3 nucleosome sites are accompanied by a low density of other nucleosomes and are associated with gene activation. This observation suggests a different sequence composition of H3K4me3 from other nucleosomes. Approach: To understand the relationship between genome sequence and chromatin structure, we studied DNA sequences at histone modification sites in various human cell types. We found sequence specificity for H3K4me3, but not for other histone modifications. Using the sequence specificities of H3 and H3K4me3 nucleosomes, we developed a model that computes the probability of H3K4me3 occupation at each base pair from the genome sequence context. Results: A comparison of our predictions with experimental data suggests a high performance of our method, revealing a strong association between H3K4me3 and specific genomic DNA context. The high probability of H3K4me3 occupation occurs at transcription start and termination sites, exon boundaries and binding sites of transcription regulators involved in chromatin modification activities, including histone acetylases and enhancer- and insulator-associated factors. Thus, the human genome sequence contains signatures for chromatin modifications essential for gene regulation and development. Our method may be applied to find new sequence elements functioning by chromatin modulation. Availability: Software and supplementary data are available at Bioinformatics online. Contact: misook.ha@samsung.com or wli@uchicago.edu Supplementary information: Supplementary data are available at Bioinformatics online. PMID:23511541

  16. Merging the Power of the Computer and VCR: Interactive Videotape with the Mandell Instant-Active Interface.

    Science.gov (United States)

    Brown, Scott W.

    1991-01-01

    Describes the Mandell Instant-Active Device, a microcomputer-based system that enables teachers to add questions and/or statement prompts to an existing videotape without affecting the videotape itself. Applications, procedures, student feedback, and hardware requirements are discussed. Ordering information is included. (MES)

  17. Merging the Power of the Computer and VCR: Interactive Videotape with the Mandell Instant-Active Interface.

    Science.gov (United States)

    Brown, Scott W.

    1991-01-01

    Describes the Mandell Instant-Active Device, a microcomputer-based system that enables teachers to add questions and/or statement prompts to an existing videotape without affecting the videotape itself. Applications, procedures, student feedback, and hardware requirements are discussed. Ordering information is included. (MES)

  18. Effect of stearic acid on the properties of metronidazole/methocel K4M floating matrices

    Directory of Open Access Journals (Sweden)

    Belem Lara-Hernández

    2009-09-01

    Full Text Available The properties of metronidazole/Methocel K4M sustained release floating tablets have been studied varying the proportion of the lubricant, stearic acid, on formulations with and without sodium bicarbonate. The variables studied include technological properties of the tablets such as tablet hardness and ejection pressure, the drug release profile, the hydration kinetics and the floating behaviour. The presence of stearic acid and sodium bicarbonate improves the floating behaviour for more than 8 hours. The hydration volume, the tablet hardness and the ejection pressure decrease as the stearic acid content increases and the polymer content decreases. Drug dissolution increases with increasing proportions of stearic acid and decreasing proportions of the polymer in the tablets. The presence of sodium bicarbonate extends the differences in dissolution produced by stearic acid. These results are attributed to decreasing matrices coherence with an increasing quantity of stearic acid and a reducing polymer proportion. The carbon dioxide bubbles produced by sodium bicarbonate expand the matrices facilitating the dissolution, although their presence obstructs also the diffusion path through the hydrated gel layer.Estudaram-se as propriedades de comprimidos flutuantes de metronidazol/Methocel K4M de liberação controlada, variando-se a proporção do lubrificante, ácido esteárico, nas formulações com e sem bicarbonato de sódio. As variáveis estudadas incluem propriedades tecnológicas dos comprimidos, tais como dureza, pressão de ejeção, perfil de liberação do fármaco, cinética de hidratação e comportamento de flutuação. A presença de ácido esteárico e do bicarbonato de sódio melhora o comportamento de flutuação para mais de 8 horas. O volume de hidratação, a dureza e a pressão de ejeção do comprimido decrescem à medida que o conteúdo de ácido esteárico e de polímero diminui. A dissolução do fármaco aumenta com o

  19. The Amazing COS FUV (1320 - 1460 A) Spectrum of (lambda) Vel (K4Ib-II)

    Science.gov (United States)

    Carpenter, Kenneth

    2010-01-01

    The FUV spectrum (1320-1460 A) of the K4 lb-11 supergiant (lambda) Vel was observed with the Cosmic Origins Spectrograph (COS) on HST, as part of the Ayres and Redfield Cycle 17 SNAP program "SNAPing Coronal Iron". This spectrum covers a region not previously recorded in (lambda) Vel at high resolution and, in a mere 20 minutes of exposure, reveals a treasure trove of information. It shows a wide variety of strong emission lines, superposed on a bright continuum, with contributions from both atomic and molecular species. Multiple absorptions, including numerous Ni II and Fe II lines, are visible over this continuum, which is likely generated in the chromosphere of the star. Evidence of the stellar wind is seen in the P Cygni profiles of the CII lines near 1335 A and the results of fluorescence processes are visible throughout the region. The spectrum has remarkable similarities to that of (alpha) Boo (K1.5 III), but significant differences as well, including substantial FUV continuum emission, reminiscent of the M2 Iab supergiant (alpha) Ori, but minus the CO fundamental absorption bands seen in the spectrum of the latter star. However, fluoresced CO emission is present, as in the K-giant stars (alpha) Boo and (alpha) Tau (K5 III). The presence of hot plasma in the atmosphere of the star, indicated by previous GHRS observations of Si III] and C III] lines near 1900 A and FUSE observations of O VI 1032 A, is further confirmed by the detection in this COS spectrum of the Si IV UV 1 lines near 1400 A, though both lines are contaminated by overlying fluorescent H2 emission. We present the details of this spectrum, in comparison with stars of similar temperature or luminosity and discuss the implications for the structure of, and the radiative processes active in, the outer atmospheres of these stars.

  20. Aberrant intracellular localization of H3k4me3 demonstrates an early epigenetic phenomenon in Alzheimer’s disease

    Science.gov (United States)

    Mastroeni, Diego; Delvaux, Elaine; Nolz, Jennifer; Tan, Yuyan; Grover, Andrew; Oddo, Salvatore; Coleman, Paul D.

    2016-01-01

    We have previously reported in Alzheimer’s disease (AD) the mislocalization of epigenetic molecules between the cell nucleus and the cytoplasm. We have extended our finding to include the aberrant localization of histone 3 trimethylation on lysine 4 (H3k4me3), an epigenetic mark associated with actively transcribing genes as well as those poised for transcription. These findings raise the question of where the ectopic localization of H3k4me3 fits within the cascade of cell biological events in the progression of AD. We, therefore, examined the expression and intracellular location of H3k4me3 as a function of Braak stage and also in relation to a series of tau markers that are indicative of disease state. Both lines of evidence showed that ectopic localization of H3k4me3 is early in the course of disease. Because of the known role of H3k4me3 in the expression of synaptic genes, our data suggest an epigenetic role in synaptic deficits early in the course of AD. PMID:26553823

  1. Aberrant intracellular localization of H3k4me3 demonstrates an early epigenetic phenomenon in Alzheimer's disease.

    Science.gov (United States)

    Mastroeni, Diego; Delvaux, Elaine; Nolz, Jennifer; Tan, Yuyan; Grover, Andrew; Oddo, Salvatore; Coleman, Paul D

    2015-12-01

    We have previously reported in Alzheimer's disease (AD) the mislocalization of epigenetic molecules between the cell nucleus and the cytoplasm. We have extended our finding to include the aberrant localization of histone 3 trimethylation on lysine 4 (H3k4me3), an epigenetic mark associated with actively transcribing genes as well as those poised for transcription. These findings raise the question of where the ectopic localization of H3k4me3 fits within the cascade of cell biological events in the progression of AD. We, therefore, examined the expression and intracellular location of H3k4me3 as a function of Braak stage and also in relation to a series of tau markers that are indicative of disease state. Both lines of evidence showed that ectopic localization of H3k4me3 is early in the course of disease. Because of the known role of H3k4me3 in the expression of synaptic genes, our data suggest an epigenetic role in synaptic deficits early in the course of AD. Copyright © 2015 Elsevier Inc. All rights reserved.

  2. Differential dynamics of histone H3 methylation at positions K4 and K9 in the mouse zygote

    Directory of Open Access Journals (Sweden)

    Walter Jörn

    2004-09-01

    Full Text Available Abstract Background In the mouse zygote the paternal genome undergoes dramatic structural and epigenetic changes. Chromosomes are decondensed, protamines replaced by histones and DNA is rapidly and actively demethylated. The epigenetic asymmetry between parental genomes remains at least until the 2-cell stage suggesting functional differences between paternal and maternal genomes during early cleavage stages. Results Here we analyzed the timing of histone deposition on the paternal pronucleus and the dynamics of histone H3 methylation (H3/K4mono-, H3/K4tri- and H3/K9di-methylation immediately after fertilization. Whereas maternal chromatin maintains all types of histone H3 methylation throughout the zygotic development, paternal chromosomes acquire new and unmodified histones shortly after fertilization. In the following hours we observe a gradual increase in H3/K4mono-methylation whereas H3/K4tri-methylation is not present before latest pronuclear stages. Histone H3/K9di-methylation is completely absent from the paternal pronucleus, including metaphase chromosomes of the first mitotic stage. Conclusion Parallel to the epigenetic asymmetry in DNA methylation, chromatin modifications are also different between both parental genomes in the very first hours post fertilization. Whereas methylation at H3/K4 gradually becomes similar between both genomes, H3/K9 methylation remains asymmetric.

  3. Association of MAP4K4 gene single nucleotide polymorphism with mastitis and milk traits in Chinese Holstein cattle.

    Science.gov (United States)

    Bhattarai, Dinesh; Chen, Xing; Ur Rehman, Zia; Hao, Xingjie; Ullah, Farman; Dad, Rahim; Talpur, Hira Sajjad; Kadariya, Ishwari; Cui, Lu; Fan, Mingxia; Zhang, Shujun

    2017-02-01

    The objective of the studies presented in this Research Communication was to investigate the association of single nucleotide polymorphisms present in the MAP4K4 gene with different milk traits in dairy cows. Based on previous QTL fine mapping results on bovine chromosome 11, the MAP4K4 gene was selected as a candidate gene to evaluate its effect on somatic cell count and milk traits in ChineseHolstein cows. Milk production traits including milk yield, fat percentage, and protein percentage of each cow were collected using 305 d lactation records. Association between MAP4K4 genotype and different traits and Somatic Cell Score (SCS) was performed using General Linear Regression Model of R. Two SNPs at exon 18 (c.2061T > G and c.2196T > C) with genotype TT in both SNPs were found significantly higher for somatic SCS. We found the significant effect of exon 18 (c.2061T > G) on protein percentage, milk yield and SCS. We identified SNPs at different location of MAP4K4 gene of the cattle and several of them were significantly associated with the somatic cell score and other different milk traits. Thus, MAP4K4 gene could be a useful candidate gene for selection of dairy cattle against mastitis and the identified polymorphisms might potentially be strong genetic markers.

  4. Gene-gene interaction and RNA splicing profiles of MAP2K4 gene in rheumatoid arthritis.

    Science.gov (United States)

    Shchetynsky, Klementy; Protsyuk, Darya; Ronninger, Marcus; Diaz-Gallo, Lina-Marcela; Klareskog, Lars; Padyukov, Leonid

    2015-05-01

    We performed gene-gene interaction analysis, with HLA-DRB1 shared epitope (SE) alleles for 195 SNPs within immunologically important MAP2K, MAP3K and MAP4K gene families, in 2010 rheumatoid arthritis (RA) patients and 2280 healthy controls. We found a significant statistical interaction for rs10468473 with SE alleles in autoantibody-positive RA. Individuals heterozygous for rs10468473 demonstrated higher expression of total MAP2K4 mRNA in blood, compared to A-allele homozygous. We discovered a novel, putatively translated, "cassette exon" RNA splice form of MAP2K4, differentially expressed in peripheral blood mononuclear cells from 88 RA cases and controls. Within the group of RA patients, we observed a correlation of MAP2K4 isoform expression with carried SE alleles, autoantibody, and rheumatoid factor profiles. TNF-dependent modulation of isoform expression pattern was detected in the Jurkat cell line. Our data suggest a genetic interaction between MAP2K4 and HLA-DRB1, and the importance of rs10468473 and MAP2K4 splice variants in the development of autoantibody-positive RA.

  5. Transcriptional regulation of the differentiation-linked human K4 promoter is dependent upon esophageal-specific nuclear factors.

    Science.gov (United States)

    Opitz, O G; Jenkins, T D; Rustgi, A K

    1998-09-11

    The stratified squamous epithelium comprises actively proliferating basal cells that undergo a program of differentiation accompanied by morphological, biochemical, and genetic changes. The transcriptional regulatory signals and the genes that orchestrate this switch from proliferation to differentiation can be studied through the keratin gene family. Given the localization of keratin 4 (K4) to the early differentiated suprabasal compartment and having previously demonstrated that targeted disruption of this gene in murine embryonic stem cells results in impairment of the normal differentiation program in esophageal and corneal epithelial cells, we studied the transcriptional regulation of the human K4 promoter. A panel of K4 promoter deletions were found in transient transfection assays to be predominantly active in esophageal and corneal cell lines. A critical cis-regulatory element resides between -163 and -140 bp and contains an inverted CACACCT motif. A site-directed mutated version of this motif within the K4 promoter renders it inactive, whereas the wild-type version is active in a heterologous promoter system. It specifically binds esophageal-specific zinc-dependent transcriptional factors. Our studies demonstrate that regulation of the human K4 promoter is in part mediated through tissue-specific transcriptional factors.

  6. Over-expression of histone H3K4 demethylase gene JMJ15 enhances salt tolerance in Arabidopsis

    Directory of Open Access Journals (Sweden)

    Yuan eShen

    2014-06-01

    Full Text Available Histone H3 lysine 4 trimethylation (H3K4me3 has been shown to be involved in stress-responsive gene expression and gene priming in plants. However, the role of H3K4me3 resetting in the processes is not clear. In this work we studied the expression and function of Arabidopsis H3K4 demethylase gene JMJ15. We show that the expression of JMJ15 was relatively low and was limited to a number of tissues during vegetative growth but was higher in young floral organs. Over-expression of the gene in gain-of-function mutants reduced the plant height with accumulation of lignin in stems, while the loss-of-function mutation did not produce any visible phenotype. The gain-of-function mutants showed enhanced salt tolerance, whereas the loss-of-function mutant was more sensitive to salt compared to the wild type. Transcriptomic analysis revealed that over-expression of JMJ15 down-regulated many genes which are preferentially marked by H3K4me3 and H3K4me2. Many of the down-regulated genes encode transcription regulators involved in stress responses. The data suggest that increased JMJ15 levels may regulate the gene expression program that enhances stress tolerance.

  7. Making the Case for Public-Private Child Care Partnerships: Child Care Partnership Project. [Videotape].

    Science.gov (United States)

    Finance Project, Washington, DC.

    The quality of child care in the United States has important implications for school preparedness, welfare reform, economic vitality, and the quality of family life. In this 8-minute videotape, business leaders describe why child care makes good business sense. Visuals explain the importance of early childhood for school and life success, and the…

  8. How To Dance through Time. Volume III: The Majesty of Renaissance Dance. [Videotape].

    Science.gov (United States)

    Teten, Carol

    This 42-minute VHS videotape is the third in a series of "How To Dance Through Time" videos. It highlights the intricacies of an Italian court dance suite, which mirrors the episodic changes of courtship. Nido D'Amore" (The Nest of Love) exposes the technique for all the dance suites of the era, and features The Opening (which…

  9. Success Story: The Impact of National Board Certification[R] in Charlotte-Mecklenburg Schools. [Videotape].

    Science.gov (United States)

    National Board for Professional Teaching Standards, Arlington, VA.

    This videotape describes the impact of National Board for Professional Teaching Standards (NBPTS) certification within North Carolina's Charlotte-Mecklenburg Schools. It begins with the story of an at-risk student who attributes his success to having had an NBPTS certified teacher in 5th grade. The teacher tutored him, paired him with a mentor,…

  10. Reptiles. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    1999

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. The ancestors of reptiles date back to the dinosaurs. After the dinosaurs died out, it was one of the best-adapted species that survived and…

  11. The Effect of a Videotape about Death on Bereaved Children in Family Therapy.

    Science.gov (United States)

    York, Janet B.; Weinstein, Sanford A.

    1980-01-01

    Children who had lost a family member were tested to determine the effect of a videotape about reactions to death. Those who viewed the tape were more able to discuss death in family therapy sessions than those who did not view the tape. (JAC)

  12. Robert Sylwester on Social Interaction and Brain Development. Windows to the Mind, Volume 1. [Videotape].

    Science.gov (United States)

    Sylwester, Robert

    This videotape explores the relationship between the child's social world and cognitive development. The first part of the video examines why the social environment is important to brain development. This section looks at the amount of time a child spends in a social environment, and the anatomy of the developing brain. The second part of the…

  13. Dancetime! 500 Years of Social Dance. Volume I: 15th-19th Centuries. [Videotape].

    Science.gov (United States)

    Teten, Carol

    This VHS videotape recording is the first in a two-volume series that presents 500 years of social dance, music, and fashion. It focuses on the 15th-19th centuries, including Renaissance nobility, Baroque extravagance, Regency refinement, and Victorian romanticism. Each era reflects the changing relationships between men and women through the…

  14. Evolution. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. The story of evolution starts with the study of fossils that helps scientists link today's living organisms with those of the past. Students…

  15. Video-Taped Instruction Creates Listening and Visual Memory Integration for Higher Reading and Math Scores.

    Science.gov (United States)

    Erland, Jan

    This pre-post quasi-experimental study was conducted in a public school 5th grade class to determine the effects of video-taped instruction in teaching analysis and pattern finding skills. Methodology included guidelines from Cognitive Behavior Modification, Suggestopedia, and Guilford's Structure of Intellect Model within the Kaufman and Kaufman…

  16. Insects & Other Arthropods. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. They also learn that there are more species of insects than any other animal class in the world. Insects are incredible creatures with many…

  17. Amphibians. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. Students find out about the world of amphibians as they examine their physical characteristics, environments, and life cycles, as well as…

  18. Animal Interdependency. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. No organism on Earth can exist independently. Students find out more about animal relationships such as predator/prey relationships and…

  19. Animal Behavior & Communication. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. The way an animal acts or behaves helps it get what it needs to survive. Students find out why some animal behaviors are instinctive while…

  20. Endangered & Extinct Animals. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. Due to environmental factors and human interference, many of Earth's creatures have ceased to exist or are on the verge of extinction. In…

  1. Fish. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. There are many types of fish that live in oceans, lakes, and streams. Students learn about fish characteristics and environmental habitats,…

  2. Animal Adaptations. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. As animals adapt to new environments, their bodies and life cycles may change over thousands of years. In this video, students find out about…

  3. Marine & Other Invertebrates. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. Invertebrate animals include a vast array of spineless creatures. In this video, students discover marine lifeforms such as jellyfish,…

  4. Animal Classification. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. They learn what the terms "kingdom", "phylum", and "order" mean, and discover how the 3.5 million-plus organisms found on Earth fit into…

  5. Animal Needs. Animal Life in Action[TM]. Life in Action. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. All animals need food, water, and shelter to grow, reproduce, and survive. Students learn about the needs of animals and how, over time, if…

  6. Birds. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. There are many different types of birds, over 9,000 different species. While not all birds take to the air, they all have feathers. Students…

  7. Dancetime! 500 Years of Social Dance. Volume II: 20th Century. [Videotape].

    Science.gov (United States)

    Teten, Carol

    This 50-minute VHS videotape is the second in a 2-volume series that presents 500 years of social dance, music, and fashion. It features dance and music of the 20th century, including; 1910s: animal dances, castle walk, apache, and tango; 1920s: black bottom and charleston; 1930s: marathon, movie musicals, big apple, and jitterbug; 1940s: rumba;…

  8. Dancetime! 500 Years of Social Dance. Volume I: 15th-19th Centuries. [Videotape].

    Science.gov (United States)

    Teten, Carol

    This VHS videotape recording is the first in a two-volume series that presents 500 years of social dance, music, and fashion. It focuses on the 15th-19th centuries, including Renaissance nobility, Baroque extravagance, Regency refinement, and Victorian romanticism. Each era reflects the changing relationships between men and women through the…

  9. Using Videotapes to Study the Construction of Mathematical Knowledge by Individual Children Working in Groups.

    Science.gov (United States)

    Davis, Robert B.; And Others

    1992-01-01

    Describes research project utilizing video cameras to record behavior while students worked on a combinatorics task in second grade and five months later when they were in third grade. Results describe student development of new mathematical ideas over an extended period of time. Authors argue that videotaping can play an important role in making…

  10. Dancetime! 500 Years of Social Dance. Volume II: 20th Century. [Videotape].

    Science.gov (United States)

    Teten, Carol

    This 50-minute VHS videotape is the second in a 2-volume series that presents 500 years of social dance, music, and fashion. It features dance and music of the 20th century, including; 1910s: animal dances, castle walk, apache, and tango; 1920s: black bottom and charleston; 1930s: marathon, movie musicals, big apple, and jitterbug; 1940s: rumba;…

  11. Evaluation of a Videotape Technique for Measuring Clinical Psychiatric Skills of Medical Students

    Science.gov (United States)

    Tardiff, Kenneth; And Others

    1978-01-01

    A technique developed at SUNY at Stony Brook measures changes in second-year medical students' ability to recognize psychopathology following an educational program in psychobiology. It compares ratings of videotaped interviews by the students to the ratings of the interviews made by the faculty preceptors participating in the teaching program.…

  12. A Videotape Technique for Measuring Clinical Skills: Three Years of Experience.

    Science.gov (United States)

    Tardiff, Kenneth

    1981-01-01

    Use of a videotape technique to evaluate medical students' ability to observe psychopathology after a psychobiology system course is reported. Performance on this audiovisual examination was found not to be related significantly to any of the other methods of evaluation used in the course or in other courses during the first two years of medical…

  13. Multi-Arts Resource Guide. Revised [and] [videotapes I and II].

    Science.gov (United States)

    Pascale, Louise

    This resource guide and the accompanying videotapes are based on the artist-generated lesson plans of the Very Special Arts Massachusetts artist residency program. Six arts units are presented: Collage, Puppetry, Movement I & II, Printmaking I & II, Theater Arts I & II, and Self Concept I & II. Each of these units consists of…

  14. How To Dance through Time. Volume IV: The Elegance of Baroque Social Dance. [Videotape].

    Science.gov (United States)

    Teten, Carol

    This 45-minute VHS videotape is the fourth in a series of "How To Dance Through Time" videos. It shows how to dance the most influential social dances of the French Baroque Court. The ceremonious Minuet is the most famous of the 18th century dance invention. The Allemande is the pivotal bridge linking to the 19th century. The Contredance…

  15. Problem-Solving Skills in Happy and Distressed Couples: Effects of Videotape and Verbal Feedback.

    Science.gov (United States)

    Fichten, Catherine S.; Wright, John

    1983-01-01

    Investigated communication in happy and distressed married couples (N=58) in two studies. Problem-solving behaviors were evaluated by the spouses and observers. Half of the distressed couples received written feedback. Results indicated both happy and distressed wives engaged in more negative behaviors than husbands, and videotape and written…

  16. Animal Behavior & Communication. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. The way an animal acts or behaves helps it get what it needs to survive. Students find out why some animal behaviors are instinctive while…

  17. All about Endangered and Extinct Animals. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    While there are thousands of different animals in the world, some have been extinct for many years and others are on the verge of extinction. In this videotape, students learn about the natural and man-made factors that lead to the endangerment and extinction of animals. Children find out why it is essential for people to help all forms of…

  18. Endangered & Extinct Animals. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. Due to environmental factors and human interference, many of Earth's creatures have ceased to exist or are on the verge of extinction. In…

  19. Presenting Social Issues with Videotape [and] Teaming Up to Take a Risk.

    Science.gov (United States)

    Agosta, Diana; Jackson, Dick

    1991-01-01

    Two articles discuss the use of media in schools. One describes the use of videotapes to present social issues; the second describes the use of an integrated learning system with ninth and tenth grade at-risk students to improve their rate of attendance, academic achievement, and self-esteem. (LRW)

  20. How To Dance through Time. Volume II: Dances of the Ragtime Era, 1910-1920. [Videotape].

    Science.gov (United States)

    Teten, Carol

    This 59-minute VHS videotape is the second in a series of "How To Dance Through Time" videos. It provides 44 step combinations and how-to instructions to help viewers learn to dance the most popular dances of the early 20th century (the ragtime era), including: the wild animal dances (fox trot, horse trot, kangaroo hop, duck waddle, squirrel,…

  1. CTEPP STANDARD OPERATING PROCEDURE FOR TRANSLATING VIDEOTAPES OF CHILD ACTIVITIES (SOP-4.13)

    Science.gov (United States)

    The EPA will conduct a two-day video translation workshop to demonstrate to coders the procedures for translating the activity patterns of preschool children on videotape. The coders will be required to pass reliability tests to successfully complete the training requirements of ...

  2. Behavioral Change as a Result of Videotaped Playback: An Examination of Two Models.

    Science.gov (United States)

    Ronchi, Don; Ripple, Richard E.

    The literature on the behavior effects of videotaped playback reveals that little theoretical formulation has been offered to explain the positive results which have been reported. Two theoretical models are considered in regard to these results. The first, a reinforcement model, suggests that some behaviors are reinforced positively and some…

  3. Canada: A Videotape Collection Focused on Bullying, Homophobia, and Queer Youth

    Science.gov (United States)

    Rofes, Eric

    2005-01-01

    This first essay presents and reviews a collection of six videotapes produced over the past decade that focus primarily on bullying, homophobia, and LGBTQ youth. The second review details a video resource for librarians and those supportive of non-censorship of library resources to provide materials and services to GLBT&Q adolescents.

  4. Darwin's Revolution in Thought: An Illustrated Lecture. Teaching Guide and Videotape.

    Science.gov (United States)

    Gould, Stephen Jay

    "Darwin's Revolution in Thought" is Stephen Jay Gould's definitive treatise on Charles Darwin. This 50-minute classroom edition videotaped lecture is structured in the form of a paradox and three riddles about Darwin's life. Each is designed to shed light on one of the key features of the theory of natural selection, its philosophical…

  5. Animal Life Cycles. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. The stages of life that animals pass through--birth, growth, maturation, reproduction, and death--make up the life cycle. Students learn…

  6. SET DOMAIN GROUP701 encodes a H3K4-methytransferase and regulates multiple key processes of rice plant development.

    Science.gov (United States)

    Liu, Kunpeng; Yu, Yu; Dong, Aiwu; Shen, Wen-Hui

    2017-07-01

    Chromatin-based epigenetic information plays an important role in developmental gene regulation, in response to environment, and in natural variation of gene expression levels. Histone H3 lysine 4 di/trimethylation (H3K4me2/3) is abundant in euchromatin and is generally associated with transcriptional activation. Strikingly, however, enzymes catalyzing H3K4me2/3 remain poorly characterized in crops so far. Here, we investigated the function of the rice SET DOMAIN GROUP 701 (SDG701) gene by molecular and biochemical characterization of the gene product, and by studying effects of its loss or gain of function on plant growth and development. We demonstrated that SDG701 encodes a methytransferase specifically catalyzing H3K4 methylation. Overexpression and knockdown experiments showed that SDG701 is crucial for proper sporophytic plant development as well as for gametophytic transmission that directly impacts rice grain production. In-depth analysis of plant flowering time revealed that SDG701 promotes rice flowering under either long-day or short-day photoperiods. Consistently, the SDG701 protein was found to bind chromatin to promote H3K4me3 and to enhance expression of the rice Hd3a and RFT1 florigens. Collectively, our results establish SDG701 as a major rice H3K4-specific methyltransferase and provide important insights into function of H3K4me3 deposition in transcription activation of florigens in promoting plant flowering. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

  7. Aberrant intracellular localization of H3k4me3 demonstrates an early epigenetic phenomenon in Alzheimer’s disease

    OpenAIRE

    Mastroeni, Diego; Delvaux, Elaine; Nolz, Jennifer; Tan, Yuyan; Grover, Andrew; Oddo, Salvatore; Coleman, Paul D.

    2015-01-01

    We have previously reported in Alzheimer’s disease (AD) the mislocalization of epigenetic molecules between the cell nucleus and the cytoplasm. We have extended our finding to include the aberrant localization of histone 3 trimethylation on lysine 4 (H3k4me3), an epigenetic mark associated with actively transcribing genes as well as those poised for transcription. These findings raise the question of where the ectopic localization of H3k4me3 fits within the cascade of cell biological events i...

  8. The effect of videotaping students' interviews with patients for interview skill education.

    Science.gov (United States)

    Lee, Woo Sung; Hwang, Ji Young; Lim, Ji Eun; Suh, Sang-Yeon; Park, Ki Heum; Sung, Nak-Jin

    2013-03-01

    The importance of communication between patients and physicians has been proven in many previous studies. The authors analyzed the effect of interview skill education through videotapes which recorded students' interviews with real patients in the outpatient department of family medicine. This study was conducted with all students who chose the elective course of family medicine and one randomly selected student every week from an 'infectious internal medicine' class at Dongguk University Ilsan Hospital during the period from December 2008 to March 2011. All students performed a preliminary examination of a new patient at the outpatient department of family medicine. All consultations were videotaped. Feedback to the student was given on the same day by viewing the videotape together. After feedback, all students performed another preliminary examination of one new patient at the department of family medicine the same week. Three family medicine residents scored all videotapes using 10-item interview skill checklists. Many parts of the checklists were modified using the Arizona Clinical Interview Rating Scales. Thirty-three students participated. Of 10 items, nine showed increased scores after feedback. There was a significant change in four items after feedback: 'type of question' (before 2.36 ± 0.60, after 2.73 ± 0.72), 'timeline' (before 2.82 ± 0.68, after 3.18 ± 0.73), 'positive verbal reinforcement' (before 2.24 ± 0.56, after 2.61 ± 0.90), and the total score (before 21.70 ± 2.62, after 23.39 ± 3.13) (P skills using videotapes of students' preliminary consultations with real patients in outpatient settings, was effective in improving the interview areas of 'type of question,' 'timeline,' 'positive verbal reinforcement,' and the total interview scores.

  9. Analisis Cakupan Antenatal Care K4 Program Kesehatan Ibu dan Anak di Wilayah Kerja Dinas Kesehatan Kabupaten Padang Pariaman

    Directory of Open Access Journals (Sweden)

    Elmispendriya Gusna

    2016-01-01

    Full Text Available AbstrakPencapaian target K4 (kunjungan ibu hamil ke tenaga kesehatan yang dilakukan paling sedikit 4 kali selama hamil akan terlaksana jika adanya motivasi bidan di desa ditambah pembinaan dari bidan koordinator yang secara rutin dilakukan dalam bentuk supervisi. Tujuan penelitian ini adalah menganalisis cakupan antenatal care K4 program kesehatan ibu dan anak di wilayah kerja dinas kesehatan kabupaten Padang Pariaman. Penelitian ini menggunakan metode penelitian gabungan antara kuantitatif dan kualitatif. Subjek penelitian untuk penelitian kuantitatif  adalah 49 orang bidan desa, sedangkan informan untuk kualitatif adalah kepala bidang kesehatan keluarga dan Kasie kesehatan ibu dan anak dinas kesehatan kabupaten Padang Pariaman, kepala puskesmas, petugas pemegang program KIA dan bidan pengelola KIA di puskesmas Sungai Limau. Hasil penelitian ini mendapatkan lebih dari separoh (53,1% bidan desa memiliki motivasi rendah, sedangkan lebih dari separoh (67,3% bidan koordinator sudah melakukan supervisi  ke bidan desa dan pada umumnya (91,8% responden memiliki cakupan K4 yang rendah. Tidak terdapat hubungan antara motivasi bidan desa dan supervisi bidan koordinator dengan cakupan antenatal care K4 (p < 0.05. Cakupan antenatal care K4 di Kabupaten Padang Pariaman belum berhasil karena kurangnya peran aktif bidan desa di tengah masyarakat, monitoring dan evaluasi dari dinas kesehatan dan pimpinan puskesmas serta supervisi bidan koordinator belum optimal, serta sumber daya manusia yang belum memaksimalkan perannya dalam melaksanakan tugas ditambah masih kurangnya  kelengkapan sarana dan prasarana. Perlu optimalisasi peran dan fungsi bidan di desa dan optimalisasi kemitraan dengan berbagai pihak dalam komunitas.Kata kunci: cakupan antenatal care K4, motivasi, supervisi AbstractThe achievement of K4 (four time antenatal care target will be success with support from motivation of village midwives and routine supervision of coordinator midwives. The

  10. The impact of a clinic-based educational videotape on knowledge and treatment behavior of men with gonorrhea.

    Science.gov (United States)

    Solomon, M Z; DeJong, W

    1988-01-01

    The impact of a ten-minute, soap opera-style videotape on knowledge and treatment behavior of black men with gonorrhea was tested in randomized clinical trials. The videotape, shown to individual patients after diagnosis, produced a sizeable increase in the percentage of patients who returned for their test-of-cure examination: while only 43.3% of control group patients returned, 53.5% of those in the videotape group did so; chi 2(1) = 9.0; P less than 0.003. In a follow-up study, similar results were found: videotape group (59.0%), control group (39.1%); chi 2(1) = 6.2; P less than 0.015. The videotape also produced large, significant increases in patient knowledge, as measured by a test administered during patients' clinic visit (P less than 0.0001). No measureable effect of the videotape was detected on patients' willingness to refer sexual contacts for treatment. The findings suggest that clinic-based educational materials, particularly soap opera-style videotapes that engender audience identification, create emotional significance for the viewer, and model requisite communication skills, may have a significant role to play in control of sexually transmitted diseases.

  11. Draft Genome Sequence of Halomonas elongata Strain K4, an Endophytic Growth-Promoting Bacterium Enhancing Salinity Tolerance In Planta

    KAUST Repository

    Lafi, Feras Fawzi

    2016-11-04

    Halomonas elongata strain K4 is an endophytic bacterial strain that was isolated from roots of Cyperus conglomeratus collected at the Red Sea coast in Thuwal, Saudi Arabia. Here, we present a draft genome sequence of this strain, highlighting a number of pathways involved in plant growth promotion under salt stress.

  12. Draft Genome Sequence of Halomonas elongata Strain K4, an Endophytic Growth-Promoting Bacterium Enhancing Salinity Tolerance In Planta

    Science.gov (United States)

    Lafi, Feras F.; Ramirez-Prado, Juan S.; Alam, Intikhab; Bajic, Vladimir B.

    2016-01-01

    Halomonas elongata strain K4 is an endophytic bacterial strain that was isolated from roots of Cyperus conglomeratus collected at the Red Sea coast in Thuwal, Saudi Arabia. Here, we present a draft genome sequence of this strain, highlighting a number of pathways involved in plant growth promotion under salt stress. PMID:27811099

  13. An efficient solvent-free synthesis of imidazolines and benzimidazoles using K 4[Fe(CN 6] catalysis

    Directory of Open Access Journals (Sweden)

    Kabeer A. Shaikh

    2012-01-01

    Full Text Available Imidazolines and Benzimidazoles have been efficiently synthesized in high yields by treatment of 1,2-diamine with aldehydes using the metal co-ordinate complex K 4[Fe(CN 6] as a catalysis. The method was carried out under solvent free condition via oxidation of carbon-nitrogen bond. The process is green, mild and inexpensive.

  14. The Structure of a High-Affinity Kainate Receptor: GluK4 Ligand-Binding Domain Crystallized with Kainate.

    Science.gov (United States)

    Kristensen, Ole; Kristensen, Lise Baadsgaard; Møllerud, Stine; Frydenvang, Karla; Pickering, Darryl S; Kastrup, Jette Sandholm

    2016-09-01

    Ionotropic glutamate receptors play a key role in fast neurotransmission in the CNS and have been linked to several neurological diseases and disorders. One subfamily is the kainate receptors, which are grouped into low-affinity (GluK1-3) and high-affinity (GluK4-5) receptors based on their affinity for kainate. Although structures of the ligand-binding domain (LBD) of all low-affinity kainate receptors have been reported, no structures of the high-affinity receptor subunits are available. Here, we present the X-ray structure of GluK4-LBD with kainate at 2.05 Å resolution, together with thermofluor and radiolabel binding affinity data. Whereas binding-site residues in GluK4 are most similar to the AMPA receptor subfamily, the domain closure and D1-D2 interlobe contacts induced by kainate are similar to the low-affinity kainate receptor GluK1. These observations provide a likely explanation for the high binding affinity of kainate at GluK4-LBD.

  15. Computational inference of H3K4me3 and H3K27ac domain length

    Directory of Open Access Journals (Sweden)

    Julian Zubek

    2016-03-01

    Full Text Available Background. Recent epigenomic studies have shown that the length of a DNA region covered by an epigenetic mark is not just a byproduct of the assaying technologies and has functional implications for that locus. For example, expanded regions of DNA sequences that are marked by enhancer-specific histone modifications, such as acetylation of histone H3 lysine 27 (H3K27ac domains coincide with cell-specific enhancers, known as super or stretch enhancers. Similarly, promoters of genes critical for cell-specific functions are marked by expanded H3K4me3 domains in the cognate cell type, and these can span DNA regions from 4–5kb up to 40–50kb in length. These expanded H3K4me3 domains are known as buffer domains or super promoters. Methods. To ask what correlates with—and potentially regulates—the length of loci marked with these two important histone marks, H3K4me3 and H3K27ac, we built Random Forest regression models. With these models, we computationally identified genomic and epigenomic patterns that are predictive for the length of these marks in seven ENCODE cell lines. Results. We found that certain epigenetic marks and transcription factors explain the variability of the length of H3K4me3 and H3K27ac marks across different cell types, which implies that the lengths of these two epigenetic marks are tightly regulated in a given cell type. Our source code for the regression models and data can be found at our GitHub page: https://github.com/zubekj/broad_peaks. Discussion. Our Random Forest based regression models enabled us to estimate the individual contribution of different epigenetic marks and protein binding patterns to the length of H3K4me3 and H3K27ac deposition patterns, therefore potentially revealing genomic signatures at cell specific regulatory elements.

  16. Teaching critical management skills to senior nursing students: videotaped or interactive hands-on instruction?

    Science.gov (United States)

    Baxter, Pamela; Akhtar-Danesh, Noori; Landeen, Janet; Norman, Geoff

    2012-01-01

    This study examined and compared the effectiveness of videotape training versus hands-on instruction in preparing senior nursing students to respond to emergency clinical situations. Fourth year nursing students (n=27) were randomly assigned to one of three groups; one group received videotaped instruction, one group engaged in a hands-on experience, and one group, a control, received no instruction. Students were evaluated using a three-station objective structured clinical examination that involved high-fidelity simulations. Differences between the control and the two instructional groups were significant (p = .007); however, there was no significant difference between the two types of instruction. It was concluded that instruction on crisis management with a high-fidelity simulator, using either video or hands-on instruction, can result in a significant improvement in performance.

  17. Childhood videotaped social and neuromotor precursors of schizophrenia: a prospective investigation

    DEFF Research Database (Denmark)

    Schiffman, Jason; Walker, Elaine; Ekstrøm, Morten;

    2004-01-01

    OBJECTIVE: The authors examined videotaped behaviors of children who developed schizophrenia as adults and of comparison subjects to disclose possible social and neuromotor deficits foreshadowing later development of schizophrenia. METHOD: In 1972, a sample of 265 11-13-year-old Danish children...... were filmed under standardized conditions while they were eating lunch. The examination was part of a larger study investigating early signs of schizophrenia spectrum disorders. Many of the subjects had a parent with schizophrenia, leaving them at high risk for developing a schizophrenia spectrum...... disorder and children who developed other psychiatric disorders. RESULTS: The findings from this study suggest that the brief videotaped footage of children eating lunch was able to discriminate between the individuals who later developed schizophrenia and those who did not. Specifically...

  18. Effects of the Videotape Recorder on Levels of Anxiety, Exhibitionism, and Reticence in High School Speech Students.

    Science.gov (United States)

    Lake, Robert L.; Adams, W. Clifton

    1984-01-01

    Concluded that the videotape recorder could be used effectively as an instructional feedback tool without fear of serious negative effects on speaker performance or on the emotional condition of students. (PD)

  19. Videotaped Feedback Method to Enhance Learning in Preclinical Operative Dentistry: An Experimental Study.

    Science.gov (United States)

    Shah, Dipali Yogesh; Dadpe, Ashwini Manish; Kalra, Dheeraj Deepak; Garcha, Vikram P

    2015-12-01

    The aim of this study was to investigate if a videotaped feedback method enhanced teaching and learning outcomes in a preclinical operative laboratory setting for novice learners. In 2013, 60 dental students at a dental school in India were randomly assigned to two groups: control (n=30) and experimental (n=30). The control group prepared a Class II tooth preparation for amalgam after receiving a video demonstration of the exercise. The experimental group received the same video demonstration as the control group, but they also participated in a discussion and analysis of the control groups' videotaped performance and then performed the same exercise. The self-evaluation scores (SS) and examiner evaluation scores (ES) of the two groups were compared using the unpaired t-test. The experimental group also used a five-point Likert scale to rate each item on the feedback form. The means of SS (13.65±2.43) and ES (14.75±1.97) of the experimental group were statistically higher than the means of SS (11.55±2.09) and ES (11.60±1.82) of the control group. Most students in the experimental group perceived that this technique enhanced their learning experience. Within the limits of this study, the videotaped feedback using both ideal and non-ideal examples enhanced the students' performance.

  20. Histone H3R2 Symmetric Dimethylation and Histone H3K4 Trimethylation Are Tightly Correlated in Eukaryotic Genomes

    OpenAIRE

    Chih-Chi Yuan; Adam G.W. Matthews; Yi Jin; Chang Feng Chen; Brad A. Chapman; Toshiro K. Ohsumi; Karen C. Glass; Tatiana G. Kutateladze; Mark L. Borowsky; Kevin Struhl; Marjorie A. Oettinger

    2012-01-01

    The preferential in vitro interaction of the PHD finger of RAG2, a subunit of the V(D)J recombinase, with histone H3 tails simultaneously trimethylated at lysine 4 and symmetrically dimethylated at arginine 2 (H3R2me2sK4me3) predicted the existence of the previously unknown histone modification H3R2me2s. Here, we report the in vivo identification of H3R2me2s . Consistent with the binding specificity of the RAG2 PHD finger, high levels of H3R2me2sK4me3 are found at antigen receptor gene segmen...

  1. Characterization and analysis of Devonian shales as related to release of gaseous hydrocarbons. Well K-4 Johnson County, Kentucky

    Energy Technology Data Exchange (ETDEWEB)

    Kalyoncu, R.S.; Snyder, M.J.

    1979-08-15

    Various characterization tasks were performed on 54 cored shale samples from Johnson County, Kentucky. Core samples were obtained from depths of 967 to 1510 feet. A total of 126 samples were canned for several DOE contractors, including Battelle Columbus. Free gas analyses indicate the presence of significant quantities of higher chain hydrocarbon gases in the K-4 shales. Hydrocarbon gas release rates and kinetic studies indicate that diffusion coefficients are inversely proportional to the square root of the molecular weight of diffusing species. Although wide scatter is observed in the chemical and physical characterization data, good correlations exist between the hydrocarbon gas contents and various laboratory characterization values (physical and chemical). A number of one-to-one relationships are pointed out and discussed. Lithologically, inorganic portions of K-4 shales are composed predominantly of quartz and illite with small quantities of pyrite and various carbonate minerals.

  2. An expedient Pd/DBU mediated cyanation of aryl/heteroaryl bromides with K4[Fe(CN)6].

    Science.gov (United States)

    Zhang, Dengyou; Sun, Haifeng; Zhang, Lei; Zhou, Yu; Li, Chunpu; Jiang, Hualiang; Chen, Kaixian; Liu, Hong

    2012-03-18

    A practical Pd(PPh(3))(4)/DBU catalytic system for the synthesis of pharmaceutically relevant aminopyridine nitrile intermediates, as well as a variety of other aryl nitriles using non-toxic K(4)[Fe(CN)(6)] has been developed. The key features of our new protocol for cyanation lie in that the reaction can be carried out with readily available Pd(PPh(3))(4) under mild and green conditions, even without the assistance of other ligands.

  3. Aspectos estruturais relacionados ao processo de troca iônica no niobato lamelar K4Nb6O17

    Directory of Open Access Journals (Sweden)

    Marcos A. Bizeto

    2006-12-01

    Full Text Available The niobate with formula K4Nb6O17 has a layered structure formed by stacked negative sheets and exchangeable cations in the interlayer region. In this study we discuss some structural aspects related to the ion exchange in layered hexaniobate based on X-ray diffractometry and vibrational Raman spectroscopy data. Hexaniobate has two distinct interlayer regions and the potassium ions of one interlayer in particular are preferably exchanged by other cations, leading to an interstratified material.

  4. Methylated H3K4, a transcription-associated histone modification, is involved in the DNA damage response pathway.

    Science.gov (United States)

    Faucher, David; Wellinger, Raymund J

    2010-08-26

    Eukaryotic genomes are associated with a number of proteins such as histones that constitute chromatin. Post-translational histone modifications are associated with regulatory aspects executed by chromatin and all transactions on genomic DNA are dependent on them. Thus, it will be relevant to understand how histone modifications affect genome functions. Here we show that the mono ubiquitylation of histone H2B and the tri-methylation of histone H3 on lysine 4 (H3K4me3), both known for their involvement in transcription, are also important for a proper response of budding yeast cells to DNA damaging agents and the passage through S-phase. Cells that cannot methylate H3K4 display a defect in double-strand break (DSB) repair by non-homologous end joining. Furthermore, if such cells incur DNA damage or encounter a stress during replication, they very rapidly lose viability, underscoring the functional importance of the modification. Remarkably, the Set1p methyltransferase as well as the H3K4me3 mark become detectable on a newly created DSB. This recruitment of Set1p to the DSB is dependent on the presence of the RSC complex, arguing for a contribution in the ensuing DNA damage repair process. Taken together, our results demonstrate that Set1p and its substrate H3K4me3, which has been reported to be important for the transcription of active genes, also plays an important role in genome stability of yeast cells. Given the high degree of conservation for the methyltransferase and the histone mark in a broad variety of organisms, these results could have similar implications for genome stability mechanisms in vertebrate and mammalian cells.

  5. [The influence of Acinetobacter calcoaceticus K-4 surface-active substances on the efficiency of microbial destruction of oil pollutants].

    Science.gov (United States)

    Pyroh, T P; Antoniuk, S I; Sorokina, A I

    2009-01-01

    The possibility of the use of Acinetobacter calcoaceticus K-4 surface-active substances (SAS) for water purification from oil was shown. The efficiency of oil degradation (2.6 g/l) in the presence of SAS preparations (5-15 %) in the form of postfermentation of cultural liquid or its supernatant was established to be 81-95 %. Intensification of oil destruction was determined by SAS affecting the activity of oil-oxidizing microbial population.

  6. Synthesis and Structural Determination of Binuclear Nine-Coordinate K4[Y2(Httha)2]·14H2O

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A Y3+ complex with triethylenetetraaminehexaacetic acid was prepared and its composition and structure were determined as K4[Y2(Httha)2]*14H2O by elemental analysis and single-crystal X-ray four circle diffraction analysis. In the binuclear complex, the two YN3O6 parts all form nine-coordinate monocapped square anti prismatic structures. There are a lot of water molecules in the unit cell which form hydrogen bonds.

  7. Methylated H3K4, a transcription-associated histone modification, is involved in the DNA damage response pathway.

    Directory of Open Access Journals (Sweden)

    David Faucher

    2010-08-01

    Full Text Available Eukaryotic genomes are associated with a number of proteins such as histones that constitute chromatin. Post-translational histone modifications are associated with regulatory aspects executed by chromatin and all transactions on genomic DNA are dependent on them. Thus, it will be relevant to understand how histone modifications affect genome functions. Here we show that the mono ubiquitylation of histone H2B and the tri-methylation of histone H3 on lysine 4 (H3K4me3, both known for their involvement in transcription, are also important for a proper response of budding yeast cells to DNA damaging agents and the passage through S-phase. Cells that cannot methylate H3K4 display a defect in double-strand break (DSB repair by non-homologous end joining. Furthermore, if such cells incur DNA damage or encounter a stress during replication, they very rapidly lose viability, underscoring the functional importance of the modification. Remarkably, the Set1p methyltransferase as well as the H3K4me3 mark become detectable on a newly created DSB. This recruitment of Set1p to the DSB is dependent on the presence of the RSC complex, arguing for a contribution in the ensuing DNA damage repair process. Taken together, our results demonstrate that Set1p and its substrate H3K4me3, which has been reported to be important for the transcription of active genes, also plays an important role in genome stability of yeast cells. Given the high degree of conservation for the methyltransferase and the histone mark in a broad variety of organisms, these results could have similar implications for genome stability mechanisms in vertebrate and mammalian cells.

  8. K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidase.

    Science.gov (United States)

    Kobza, Keyna; Camporeale, Gabriela; Rueckert, Brian; Kueh, Alice; Griffin, Jacob B; Sarath, Gautam; Zempleni, Janos

    2005-08-01

    Histones are modified post-translationally, e.g. by methylation of lysine and arginine residues, and by phosphorylation of serine residues. These modifications regulate processes such as gene expression, DNA repair, and mitosis and meiosis. Recently, evidence has been provided that histones are also modified by covalent binding of the vitamin biotin. The aims of this study were to identify biotinylation sites in histone H3, and to investigate the crosstalk among histone biotinylation, methylation and phosphorylation. Synthetic peptides based on the sequence of human histone H3 were used as substrates for enzymatic biotinylation by biotinidase; biotin in peptides was probed using streptavidin peroxidase. These studies provided evidence that K4, K9 and K18 in histone H3 are good targets for biotinylation; K14 and K23 are relatively poor targets. Antibodies were generated to histone H3, biotinylated either at K4, K9 or K18. These antibodies localized to nuclei in human placental cells in immunocytochemistry and immunoblotting experiments, suggesting that lysines in histone H3 are biotinylated in vivo. Dimethylation of R2, R8 and R17 increased biotinylation of K4, K9 and K18, respectively, by biotinidase; phosphorylation of S10 abolished biotinylation of K9. These observations are consistent with crosstalk between biotinylation of histones and other known modifications of histones. We speculate that this crosstalk provides a link to known roles for biotin in gene expression and cell proliferation.

  9. PHF20 Readers Link Methylation of Histone H3K4 and p53 with H4K16 Acetylation

    Directory of Open Access Journals (Sweden)

    Brianna J. Klein

    2016-10-01

    Full Text Available PHF20 is a core component of the lysine acetyltransferase complex MOF (male absent on the first-NSL (non-specific lethal that generates the major epigenetic mark H4K16ac and is necessary for transcriptional regulation and DNA repair. The role of PHF20 in the complex remains elusive. Here, we report on functional coupling between methylation readers in PHF20. We show that the plant homeodomain (PHD finger of PHF20 recognizes dimethylated lysine 4 of histone H3 (H3K4me2 and represents an example of a native reader that selects for this modification. Biochemical and structural analyses help to explain this selectivity and the preference of Tudor2, another reader in PHF20, for dimethylated p53. Binding of the PHD finger to H3K4me2 is required for histone acetylation, accumulation of PHF20 at target genes, and transcriptional activation. Together, our findings establish a unique PHF20-mediated link between MOF histone acetyltransferase (HAT, p53, and H3K4me2, and suggest a model for rapid spreading of H4K16ac-enriched open chromatin.

  10. PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3.

    Science.gov (United States)

    Chung, Ho-Ryun; Xu, Chao; Fuchs, Alisa; Mund, Andreas; Lange, Martin; Staege, Hannah; Schubert, Tobias; Bian, Chuanbing; Dunkel, Ilona; Eberharter, Anton; Regnard, Catherine; Klinker, Henrike; Meierhofer, David; Cozzuto, Luca; Winterpacht, Andreas; Di Croce, Luciano; Min, Jinrong; Will, Hans; Kinkley, Sarah

    2016-05-25

    PHF13 is a chromatin affiliated protein with a functional role in differentiation, cell division, DNA damage response and higher chromatin order. To gain insight into PHF13's ability to modulate these processes, we elucidate the mechanisms targeting PHF13 to chromatin, its genome wide localization and its molecular chromatin context. Size exclusion chromatography, mass spectrometry, X-ray crystallography and ChIP sequencing demonstrate that PHF13 binds chromatin in a multivalent fashion via direct interactions with H3K4me2/3 and DNA, and indirectly via interactions with PRC2 and RNA PolII. Furthermore, PHF13 depletion disrupted the interactions between PRC2, RNA PolII S5P, H3K4me3 and H3K27me3 and resulted in the up and down regulation of genes functionally enriched in transcriptional regulation, DNA binding, cell cycle, differentiation and chromatin organization. Together our findings argue that PHF13 is an H3K4me2/3 molecular reader and transcriptional co-regulator, affording it the ability to impact different chromatin processes.

  11. Enhancer-associated H3K4 monomethylation by trithorax-related, the drosophila homolog of mammalian MLL3/MLL4

    NARCIS (Netherlands)

    H.-M. Herz (Hans-Martin); M. Mohan (Man); A.S. Garruss (Alexander); K. Liang (Kaiwei); Y.-H. Takahashi (Yoh-hei); K. Mickey (Kristen); O. Voets (Olaf); C.P. Verrijzer (Peter); A. Shilatifard (Ali)

    2012-01-01

    textabstractMonomethylation of histone H3 on Lys 4 (H3K4me1) and acetylation of histone H3 on Lys 27 (H3K27ac) are histone modifications that are highly enriched over the body of actively transcribed genes and on enhancers. Although in yeast all H3K4 methylation patterns, including H3K4me1, are impl

  12. Morphed and moving: TNFα-driven motility promotes cell dissemination through MAP4K4-induced cytoskeleton remodeling

    Directory of Open Access Journals (Sweden)

    Min Ma

    2014-04-01

    Full Text Available Cell dissemination from an initial site of growth is a highly coordinated and controlled process that depends on cell motility. The mechanistic principles that orchestrate cell motility, namely cell shape control, traction and force generation, are highly conserved between cells of different origins. Correspondingly, the molecular mechanisms that regulate these critical aspects of migrating cells are likely functionally conserved too. Thus, cell motility deregulation of unrelated pathogenesis could be caused and maintained by similar mechanistic principles. One such motility deregulation disorder is the leukoproliferative cattle disease Tropical Theileriosis, which is caused by the intracellular, protozoan parasite Theileria annulata. T. annulata transforms its host cell and promotes the dissemination of parasite-infected cells throughout the body of the host. An analogous condition with a fundamentally different pathogenesis is metastatic cancer, where oncogenically transformed cells disseminate from the primary tumor to form distant metastases. Common to both diseases is the dissemination of motile cells from the original site. However, unlike metastatic cancer, host cell transformation by Theileria parasites can be reverted by drug treatment and cell signaling be analyzed under transformed and non-transformed conditions. We have used this reversible transformation model and investigated parasite control of host cell motile properties in the context of inflammatory signaling in Ma M. et al. [PLoS Pathog (2014 10: e1004003]. We found that parasite infection promotes the production of the inflammatory cytokine TNFα in the host macrophage. We demonstrated that increased TNFα triggers motile and invasive properties by enhancing actin cytoskeleton remodeling and cell motility through the ser/thr kinase MAP4K4. We concluded that inflammatory conditions resulting in increased TNFα could facilitate cell dissemination by activating the actin

  13. Association of common genetic variants in the MAP4K4 locus with prediabetic traits in humans.

    Directory of Open Access Journals (Sweden)

    Tina Sartorius

    Full Text Available Mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4 is expressed in all diabetes-relevant tissues and mediates cytokine-induced insulin resistance. We investigated whether common single nucleotide polymorphisms (SNPs in the MAP4K4 locus associate with glucose intolerance, insulin resistance, impaired insulin release, or elevated plasma cytokines. The best hit was tested for association with type 2 diabetes. Subjects (N = 1,769 were recruited from the Tübingen Family (TÜF study for type 2 diabetes and genotyped for tagging SNPs. In a subgroup, cytokines were measured. Association with type 2 diabetes was tested in a prospective case-cohort study (N = 2,971 derived from the EPIC-Potsdam study. Three SNPs (rs6543087, rs17801985, rs1003376 revealed nominal and two SNPs (rs11674694, rs11678405 significant associations with 2-hour glucose levels. SNPs rs6543087 and rs11674694 were also nominally associated with decreased insulin sensitivity. Another two SNPs (rs2236936, rs2236935 showed associations with reduced insulin release, driven by effects in lean subjects only. Three SNPs (rs11674694, rs13003883, rs2236936 revealed nominal associations with IL-6 levels. SNP rs11674694 was significantly associated with type 2 diabetes. In conclusion, common variation in MAP4K4 is associated with insulin resistance and β-cell dysfunction, possibly via this gene's role in inflammatory signalling. This variation's impact on insulin sensitivity may be more important since its effect on insulin release vanishes with increasing BMI.

  14. The Oryza sativa Regulator HDR1 Associates with the Kinase OsK4 to Control Photoperiodic Flowering.

    Directory of Open Access Journals (Sweden)

    Xuehui Sun

    2016-03-01

    Full Text Available Rice is a facultative short-day plant (SDP, and the regulatory pathways for flowering time are conserved, but functionally modified, in Arabidopsis and rice. Heading date 1 (Hd1, an ortholog of Arabidopsis CONSTANS (CO, is a key regulator that suppresses flowering under long-day conditions (LDs, but promotes flowering under short-day conditions (SDs by influencing the expression of the florigen gene Heading date 3a (Hd3a. Another key regulator, Early heading date 1 (Ehd1, is an evolutionarily unique gene with no orthologs in Arabidopsis, which acts as a flowering activator under both SD and LD by promoting the rice florigen genes Hd3a and RICE FLOWERING LOCUST 1 (RFT1. Here, we report the isolation and characterization of the flowering regulator Heading Date Repressor1 (HDR1 in rice. The hdr1 mutant exhibits an early flowering phenotype under natural LD in a paddy field in Beijing, China (39°54'N, 116°23'E, as well as under LD but not SD in a growth chamber, indicating that HDR1 may functionally regulate flowering time via the photoperiod-dependent pathway. HDR1 encodes a nuclear protein that is most active in leaves and floral organs and exhibits a typical diurnal expression pattern. We determined that HDR1 is a novel suppressor of flowering that upregulates Hd1 and downregulates Ehd1, leading to the downregulation of Hd3a and RFT1 under LDs. We have further identified an HDR1-interacting kinase, OsK4, another suppressor of rice flowering under LDs. OsK4 acts similarly to HDR1, suppressing flowering by upregulating Hd1 and downregulating Ehd1 under LDs, and OsK4 can phosphorylate HD1 with HDR1 presents. These results collectively reveal the transcriptional regulators of Hd1 for the day-length-dependent control of flowering time in rice.

  15. The Oryza sativa Regulator HDR1 Associates with the Kinase OsK4 to Control Photoperiodic Flowering.

    Science.gov (United States)

    Sun, Xuehui; Zhang, Zhiguo; Wu, Jinxia; Cui, Xuean; Feng, Dan; Wang, Kai; Xu, Ming; Zhou, Li; Han, Xiao; Gu, Xiaofeng; Lu, Tiegang

    2016-03-01

    Rice is a facultative short-day plant (SDP), and the regulatory pathways for flowering time are conserved, but functionally modified, in Arabidopsis and rice. Heading date 1 (Hd1), an ortholog of Arabidopsis CONSTANS (CO), is a key regulator that suppresses flowering under long-day conditions (LDs), but promotes flowering under short-day conditions (SDs) by influencing the expression of the florigen gene Heading date 3a (Hd3a). Another key regulator, Early heading date 1 (Ehd1), is an evolutionarily unique gene with no orthologs in Arabidopsis, which acts as a flowering activator under both SD and LD by promoting the rice florigen genes Hd3a and RICE FLOWERING LOCUST 1 (RFT1). Here, we report the isolation and characterization of the flowering regulator Heading Date Repressor1 (HDR1) in rice. The hdr1 mutant exhibits an early flowering phenotype under natural LD in a paddy field in Beijing, China (39°54'N, 116°23'E), as well as under LD but not SD in a growth chamber, indicating that HDR1 may functionally regulate flowering time via the photoperiod-dependent pathway. HDR1 encodes a nuclear protein that is most active in leaves and floral organs and exhibits a typical diurnal expression pattern. We determined that HDR1 is a novel suppressor of flowering that upregulates Hd1 and downregulates Ehd1, leading to the downregulation of Hd3a and RFT1 under LDs. We have further identified an HDR1-interacting kinase, OsK4, another suppressor of rice flowering under LDs. OsK4 acts similarly to HDR1, suppressing flowering by upregulating Hd1 and downregulating Ehd1 under LDs, and OsK4 can phosphorylate HD1 with HDR1 presents. These results collectively reveal the transcriptional regulators of Hd1 for the day-length-dependent control of flowering time in rice.

  16. Activity measurements of the radionuclides 18F and 99mTc for the NMISA, South Africa in the ongoing comparisons BIPM.RI(II)-K4.F-18 and BIPM.RI(II)-K4.Tc-99m

    Science.gov (United States)

    Michotte, C.; Nonis, M.; Van Rooy, M. W.; Van Staden, M. J.; Lubbe, J.

    2017-01-01

    In 2015, comparisons of activity measurements of 18F and 99mTc using the Transfer Instrument of the International Reference System (SIRTI) took place at the National Metrology Institute of South Africa (NMISA, South Africa). Ampoules containing about 25 kBq of 18F and 99mTc solutions were measured in the SIRTI for more than two half-lives. The NMISA standardized the activity in the ampoules by ionization chamber measurements traceable to 4π(LS)β-γ coincidence measurements. The comparisons, identifiers BIPM.RI(II)-K4.F-18 and BIPM.RI(II)-K4.Tc-99m, are linked to the corresponding BIPM.RI(II)-K1.F-18 and BIPM.RI(II)-K1.Tc-99m comparisons and degrees of equivalence with the respective key comparison reference values have been evaluated. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCRI, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  17. The H3K4me3/2 histone demethylase RBR-2 controls axon guidance by repressing the actin-remodeling gene wsp-1

    DEFF Research Database (Denmark)

    Mariani, Luca; Lussi, Yvonne C.; Vandamme, Julien;

    2016-01-01

    . Here, we show that RBR-2, the sole homolog of the KDM5 family of H3K4me3/2 demethylases in Caenorhabditis elegans, ensures correct axon guidance by controlling the expression of the actin regulator wsp-1. Loss of rbr-2 results in increased levels of H3K4me3 at the transcriptional start site of wsp-1...

  18. Childhood videotaped social and neuromotor precursors of schizophrenia: a prospective investigation

    DEFF Research Database (Denmark)

    Schiffman, Jason; Walker, Elaine; Ekstrøm, Morten;

    2004-01-01

    OBJECTIVE: The authors examined videotaped behaviors of children who developed schizophrenia as adults and of comparison subjects to disclose possible social and neuromotor deficits foreshadowing later development of schizophrenia. METHOD: In 1972, a sample of 265 11-13-year-old Danish children...... were filmed under standardized conditions while they were eating lunch. The examination was part of a larger study investigating early signs of schizophrenia spectrum disorders. Many of the subjects had a parent with schizophrenia, leaving them at high risk for developing a schizophrenia spectrum...

  19. Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae)

    Science.gov (United States)

    Serrano, Érica Alves; Araya-Jaime, Cristian; Suárez-Villota, Elkin Y.; Oliveira, Claudio; Foresti, Fausto

    2016-01-01

    Abstract Characidium gomesi Travasso, 1956 specimens from the Pardo River have up to four heterochromatic supernumerary chromosomes, derived from the sex chromosomes. To access the meiotic behavior and distribution of an active chromatin marker, males and females of Characidium gomesi with two or three B chromosomes were analyzed. Mitotic chromosomes were characterized using C-banding and FISH with B chromosome probes. Meiocytes were subjected to immunofluorescence-FISH assay using anti-SYCP3, anti-H3K4m, and B chromosomes probes. Molecular homology of supernumeraries was confirmed by FISH and by its bivalent conformation in individuals with two of these chromosomes. In individuals with three Bs, these elements formed a bivalent and a univalent. Supernumerary and sex chromosomes exhibited H3K4m signals during pachytene contrasting with their heterochromatic and asynaptic nature, which suggest a more structural role than functional of this histone modification. The implications of this result are discussed in light of the homology, meiotic nuclear organization, and meiotic silencing of unsynapsed chomatin. PMID:27551347

  20. Trithorax monomethylates histone H3K4 and interacts directly with CBP to promote H3K27 acetylation and antagonize Polycomb silencing.

    Science.gov (United States)

    Tie, Feng; Banerjee, Rakhee; Saiakhova, Alina R; Howard, Benny; Monteith, Kelsey E; Scacheri, Peter C; Cosgrove, Michael S; Harte, Peter J

    2014-03-01

    Trithorax (TRX) antagonizes epigenetic silencing by Polycomb group (PcG) proteins, stimulates enhancer-dependent transcription, and establishes a 'cellular memory' of active transcription of PcG-regulated genes. The mechanisms underlying these TRX functions remain largely unknown, but are presumed to involve its histone H3K4 methyltransferase activity. We report that the SET domains of TRX and TRX-related (TRR) have robust histone H3K4 monomethyltransferase activity in vitro and that Tyr3701 of TRX and Tyr2404 of TRR prevent them from being trimethyltransferases. The trx(Z11) missense mutation (G3601S), which abolishes H3K4 methyltransferase activity in vitro, reduces the H3K4me1 but not the H3K4me3 level in vivo. trx(Z11) also suppresses the impaired silencing phenotypes of the Pc(3) mutant, suggesting that H3K4me1 is involved in antagonizing Polycomb silencing. Polycomb silencing is also antagonized by TRX-dependent H3K27 acetylation by CREB-binding protein (CBP). We show that perturbation of Polycomb silencing by TRX overexpression requires CBP. We also show that TRX and TRR are each physically associated with CBP in vivo, that TRX binds directly to the CBP KIX domain, and that the chromatin binding patterns of TRX and TRR are highly correlated with CBP and H3K4me1 genome-wide. In vitro acetylation of H3K27 by CBP is enhanced on K4me1-containing H3 substrates, and independently altering the H3K4me1 level in vivo, via the H3K4 demethylase LSD1, produces concordant changes in H3K27ac. These data indicate that the catalytic activities of TRX and CBP are physically coupled and suggest that both activities play roles in antagonizing Polycomb silencing, stimulating enhancer activity and cellular memory.

  1. K4MY METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  2. K4BM METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  3. K4BL METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  4. Quantification of video-taped images in microcirculation research using inexpensive imaging software (Adobe Photoshop).

    Science.gov (United States)

    Brunner, J; Krummenauer, F; Lehr, H A

    2000-04-01

    Study end-points in microcirculation research are usually video-taped images rather than numeric computer print-outs. Analysis of these video-taped images for the quantification of microcirculatory parameters usually requires computer-based image analysis systems. Most software programs for image analysis are custom-made, expensive, and limited in their applicability to selected parameters and study end-points. We demonstrate herein that an inexpensive, commercially available computer software (Adobe Photoshop), run on a Macintosh G3 computer with inbuilt graphic capture board provides versatile, easy to use tools for the quantification of digitized video images. Using images obtained by intravital fluorescence microscopy from the pre- and postischemic muscle microcirculation in the skinfold chamber model in hamsters, Photoshop allows simple and rapid quantification (i) of microvessel diameters, (ii) of the functional capillary density and (iii) of postischemic leakage of FITC-labeled high molecular weight dextran from postcapillary venules. We present evidence of the technical accuracy of the software tools and of a high degree of interobserver reliability. Inexpensive commercially available imaging programs (i.e., Adobe Photoshop) provide versatile tools for image analysis with a wide range of potential applications in microcirculation research.

  5. Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation

    Energy Technology Data Exchange (ETDEWEB)

    Bian, Chuanbing; Xu, Chao; Ruan, Jianbin; Lee, Kenneth K.; Burke, Tara L.; Tempel, Wolfram; Barsyte, Dalia; Li, Jing; Wu, Minhao; Zhou, Bo O.; Fleharty, Brian E.; Paulson, Ariel; Allali-Hassani, Abdellah; Zhou, Jin-Qiu; Mer, Georges; Grant, Patrick A.; Workman, Jerry L.; Zang, Jianye; Min, Jinrong (Toronto); (Stowers); (UST - China); (UV); (Chinese Aca. Sci.); (MCCM)

    2011-09-28

    The SAGA (Spt-Ada-Gcn5 acetyltransferase) complex is an important chromatin modifying complex that can both acetylate and deubiquitinate histones. Sgf29 is a novel component of the SAGA complex. Here, we report the crystal structures of the tandem Tudor domains of Saccharomyces cerevisiae and human Sgf29 and their complexes with H3K4me2 and H3K4me3 peptides, respectively, and show that Sgf29 selectively binds H3K4me2/3 marks. Our crystal structures reveal that Sgf29 harbours unique tandem Tudor domains in its C-terminus. The tandem Tudor domains in Sgf29 tightly pack against each other face-to-face with each Tudor domain harbouring a negatively charged pocket accommodating the first residue alanine and methylated K4 residue of histone H3, respectively. The H3A1 and K4me3 binding pockets and the limited binding cleft length between these two binding pockets are the structural determinants in conferring the ability of Sgf29 to selectively recognize H3K4me2/3. Our in vitro and in vivo functional assays show that Sgf29 recognizes methylated H3K4 to recruit the SAGA complex to its targets sites and mediates histone H3 acetylation, underscoring the importance of Sgf29 in gene regulation.

  6. Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2

    DEFF Research Database (Denmark)

    Pasini, Diego; Hansen, Klaus H; Christensen, Jesper;

    2008-01-01

    Polycomb group (PcG) proteins regulate important cellular processes such as embryogenesis, cell proliferation, and stem cell self-renewal through the transcriptional repression of genes determining cell fate decisions. The Polycomb-Repressive Complex 2 (PRC2) is highly conserved during evolution......, and its intrinsic histone H3 Lys 27 (K27) trimethylation (me3) activity is essential for PcG-mediated transcriptional repression. Here, we show a functional interplay between the PRC2 complex and the H3K4me3 demethylase Rbp2 (Jarid1a) in mouse embryonic stem (ES) cells. By genome-wide location analysis we...... found that Rbp2 is associated with a large number of PcG target genes in mouse ES cells. We show that the PRC2 complex recruits Rbp2 to its target genes, and that this interaction is required for PRC2-mediated repressive activity during ES cell differentiation. Taken together, these results demonstrate...

  7. KEY COMPARISON: Final report on key comparison APMP.QM-K4.1: Ethanol in nitrogen

    Science.gov (United States)

    Kato, Kenji; Maruyama, Masaaki; Seog Kim, Jin; Hyub, Oh Sang; Woo, Jin-Chun; Kim, Yongdoo; Bae, Hyunkil; Han, Qiao; Zhou, Zeyi

    2008-01-01

    The aim of this comparison was to compare capabilities for the preparation and value assignment of gas mixture standards for ethanol in nitrogen, maintained at national metrology institutes (NMIs). The measurements in this key comparison took place in 2006. There were three participants and one coordinating laboratory. The key comparison reference value (KCRV) was based on the gravimetric preparation for all components. The range of the nominal amount-of-substance fractions of the comparison standard is 100 µmol/mol to 130 µmol/mol. The Chemical Evaluation Research Institute (CERI, Japan) prepared gravimetric mixture samples of ethanol in nitrogen in 10 L aluminium cylinders and the homogeneity and long-term stability of these samples were evaluated at CERI before and after the shipment of the cylinders to the participants. Each participating laboratory was shipped one cylinder to be analyzed and then returned to the CERI. Each sample was reanalyzed to verify whether the contents were stable throughout the comparison. These measurements showed no significant change in the amount fraction within the estimated uncertainty of the measurements. Two of the three participants submitted results that were within 0.1% of the relevant reference value. In these cases, the estimated uncertainty was larger than the deviation from the reference value. CERI was the link laboratory between CCQM-K4 and APMP.QM-K4. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (MRA).

  8. High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production

    OpenAIRE

    Catapano Angela; De Rosa Mario; Cimini Donatella; Restaino Odile; Schiraldi Chiara

    2011-01-01

    Abstract Background The bacteria Escherichia coli K4 produces a capsular polysaccharide (K4 CPS) whose backbone is similar to the non sulphated chondroitin chain. The chondroitin sulphate is one of the major components of the extra-cellular matrix of the vertebrate connective tissues and a high value molecule, widely employed as active principle in the treatment of osteoarthritis. It is usually obtained by extraction from animal tissues, but the risk of virus contaminations, as well as the sc...

  9. Weissella paramesenteroides WpK4 reduces gene expression of intestinal cytokines, and hepatic and splenic injuries in a murine model of typhoid fever.

    Science.gov (United States)

    Alvim, L B; Sandes, S H C; Silva, B C; Steinberg, R S; Campos, M H A; Acurcio, L B; Arantes, R M E; Nicoli, J R; Neumann, E; Nunes, Á C

    2016-02-01

    Diarrhoea in piglets by Salmonella and other pathogens can be a serious health problem. Non-drug treatments such as probiotic microorganisms have various effects on the gastrointestinal microbiota dysbiosis and host immune system modulation. The aim of this study was to demonstrate the suitable use of Weissella paramesenteroides WpK4 strain isolated from healthy piglets as an alternative prophylactic or therapeutic treatment against Salmonella Typhimurium. Out of 37 lactic acid bacteria isolates, 24 strains belonging to the Weissella and Lactobacillus genera were analysed in vitro for desirable probiotic characteristics. The W. paramesenteroides WpK4 strain fulfilled all in vitro tests: resistance to acidic pH and bile salts, hydrophobic cell surface, antagonism against bacterial pathogens, H2O2 production and exopolysaccharide secretion, and non-transferable resistance to antibiotics. Mice fed with WpK4 showed no signs of bacterial translocation to the liver or spleen and decreased Salmonella translocation to these organs. Significantly, WpK4 intake attenuated the weight loss, fostered the preservation of intestinal architecture and integrity, and promoted survival in mice following infection with Salmonella Typhimurium. In addition, WpK4 modulated immune cellular response by inhibiting the production of pro-inflammatory cytokines and inducing anti-inflammatory mediators. These findings validate the probiotic properties of W. paramesenteroides WpK4 strain, and its eventual use in piglets.

  10. How To Start a Family Day Care = Como Iniciar una Guarderia en El Hogar. [Videotape and Viewer's Guide].

    Science.gov (United States)

    Raphael, Doris; Weisman, Douglas

    Being a family day care provider is work that requires professional responsibilities and attention to the physical, emotional, and educational well-being of children; listening and responding to parents; and running a business. This videotape, in English- and Spanish-language versions, explores the elements involved in starting up a family day…

  11. "Drugs and AIDS--reaching for help": a videotape on AIDS and drug abuse prevention for criminal justice populations.

    Science.gov (United States)

    Gross, M; DeJong, W; Lamb, D; Enos, T; Mason, T; Weitzman, E

    1994-01-01

    This article describes the development of a videotape targeted at persons under supervision of the criminal justice system. The videotape seeks to encourage those who use illicit drugs to enter drug treatment and to motivate those at risk for exposure to human immunodeficiency virus (HIV) to alter behaviors that may transmit infection. The criminal justice system presents an important opportunity to deliver such messages, particularly to a large population of persons briefly detained in a jail or lockup and released without subsequent incarceration. Evidence suggests that, even in this audience, knowledge of how to prevent exposure to HIV is widespread, yet those at risk often fail to take appropriate precautions: motivating behavior change demands more than imparting information. In order to shape this videotape, we analyzed the target audience and developed a drama-based approach that applies the framework of social learning theory, the health belief model, and principles of social marketing. This article describes the integration of that theoretical framework into the production process, content, and strategy of the videotape.

  12. An Experimental Study of the Clinical Acquisition of Behavioral Principles by Videotape Self-Confrontation. Final Report.

    Science.gov (United States)

    Denver Univ., CO.

    To determine the effect of videotape self-confrontation as a training device for speech clinicians, 30 students participated in a 12 month study. Ten experimental subjects were assigned to single confrontation, 10 to double confrontation, and 10 were control subjects. Each confrontation subject used a therapy matrix and scored his therapy session…

  13. Conjoint Consultation Using Self-Administered Manual and Videotape Parent-Teacher Training: Effects on Children's Behavioral Difficulties.

    Science.gov (United States)

    Kratochwill, Thomas R.; Elliott, Stephen N.; Loitz, Pamela Ann; Sladeczek, Ingrid; Carlson, John S.

    2003-01-01

    Research compared effectiveness of two different approaches of conjoint consultation using a manual versus a videotape series as the main components of training parents and teachers to treat children's behavioral difficulties. Parent and teacher reports characterized students as meeting their overall behavior goals; and reported high rates of…

  14. How To Dance through Time. Volume I: The Romance of Mid-19th Century Couple Dances. Beginning Level. [Videotape].

    Science.gov (United States)

    Teten, Carol

    This 35-minute VHS videotape is the first in a series of "How To Dance Through Time" videos. It provides how-to instructions to help beginning dancers learn the mid-19th century ballroom couple dances. It introduces dancers to the basic steps, which accompany the romantic dance music of the past. Each dance segment is introduced by a…

  15. 36 CFR 1280.48 - How do I apply to film, photograph, or videotape on NARA property for news purposes?

    Science.gov (United States)

    2010-07-01

    ... permission to use your own microfilming equipment to film archival records and donated historical materials... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false How do I apply to film... film, photograph, or videotape on NARA property for news purposes? (a) If you wish to film,...

  16. I Am Your Child. Quality Child Care: Making the Right Choice for You and Your Child. [Videotape].

    Science.gov (United States)

    I Am Your Child Foundation, Beverly Hills, CA.

    Finding the right child care is often challenging for parents, but it is one of the most important decisions a parent will ever make. This videotape is intended to help parents with the process of evaluating child care options. The 30-minute video is presented in seven parts. Part 1, "Choosing Child Care," discusses why quality child…

  17. Macrohistone Variants Preserve Cell Identity by Preventing the Gain of H3K4me2 during Reprogramming to Pluripotency

    Directory of Open Access Journals (Sweden)

    María J. Barrero

    2013-04-01

    Full Text Available Transcription-factor-induced reprogramming of somatic cells to pluripotency is a very inefficient process, probably due to the existence of important epigenetic barriers that are imposed during differentiation and that contribute to preserving cell identity. In an effort to decipher the molecular nature of these barriers, we followed a genome-wide approach, in which we identified macrohistone variants (macroH2A as highly expressed in human somatic cells but downregulated after reprogramming to pluripotency, as well as strongly induced during differentiation. Knockdown of macrohistone variants in human keratinocytes increased the efficiency of reprogramming to pluripotency, whereas overexpression had opposite effects. Genome-wide occupancy profiles show that in human keratinocytes, macroH2A.1 preferentially occupies genes that are expressed at low levels and are marked with H3K27me3, including pluripotency-related genes and bivalent developmental regulators. The presence of macroH2A.1 at these genes prevents the regain of H3K4me2 during reprogramming, imposing an additional layer of repression that preserves cell identity.

  18. Remarks on Vertex-Distinguishing IE-Total Coloring of Complete Bipartite Graphs K4,n and Kn,n

    Institute of Scientific and Technical Information of China (English)

    Xiang'en CHEN; Xiaoqing XIN; Wenyu HE

    2012-01-01

    Let G be a simple graph.An IE-total coloring f of G refers to a coloring of the vertices and edges of G so that no two adjacent vertices receive the same color.Let C(u) be the set of colors of vertex u and edges incident to u under f.For an IE-total coloring f of G using k colors,if C(u) ≠ C(v) for any two different vertices u and v of V(G),then f is called a k-vertex-distinguishing IE-total-coloring of G,or a k-VDIET coloring of G for short.The minimum number of colors required for a VDIET coloring of G is denoted by xievt(G),and it is called the VDIET chromatic number of G.We will give VDIET chromatic numbers for complete bipartite graph K4,n (n ≥ 4),Kn,n (5 ≤ n ≤ 21) in this article.

  19. Decreased H3K27 and H3K4 trimethylation on mortal chromosomes in distributed stem cells.

    Science.gov (United States)

    Huh, Y H; Sherley, J L

    2014-12-04

    The role of immortal DNA strands that co-segregate during mitosis of asymmetrically self-renewing distributed stem cells (DSCs) is unknown. Previously, investigation of immortal DNA strand function and molecular mechanisms responsible for their nonrandom co-segregation was precluded by difficulty in identifying DSCs and immortal DNA strands. Here, we report the use of two technological innovations, selective DSC expansion and establishment of H2A.Z chromosomal asymmetry as a specific marker of 'immortal chromosomes,' to investigate molecular properties of immortal chromosomes and opposing 'mortal chromosomes' in cultured mouse hair follicle DSCs. Although detection of the respective suppressive and activating H3K27me3 and H3K4me3 epigenetic marks on immortal chromosomes was similar to randomly segregated chromosomes, detection of both was lower on mortal chromosomes destined for lineage-committed sister cells. This global epigenomic feature of nonrandom co-segregation may reveal a mechanism that maintains an epigenome-wide 'poised' transcription state, which preserves DSC identity, while simultaneously activating sister chromosomes for differentiation.

  20. High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production

    Directory of Open Access Journals (Sweden)

    De Rosa Mario

    2011-02-01

    Full Text Available Abstract Background The bacteria Escherichia coli K4 produces a capsular polysaccharide (K4 CPS whose backbone is similar to the non sulphated chondroitin chain. The chondroitin sulphate is one of the major components of the extra-cellular matrix of the vertebrate connective tissues and a high value molecule, widely employed as active principle in the treatment of osteoarthritis. It is usually obtained by extraction from animal tissues, but the risk of virus contaminations, as well as the scarceness of raw material, makes this productive process unsafe and unable to satisfy the growing market demand. In previous studies a new biotechnological process to produce chondroitin from Escherichia coli K4 capsular polysaccharide was investigated and a 1.4 g·L-1 K4 CPS concentration was reached using fed-batch fermentation techniques. In this work, on the trail of these results, we exploited new fermentation strategies to further improve the capsular polysaccharide production. Results The inhibitory effect of acetate on the bacterial cells growth and K4 CPS production was studied in shake flask conditions, while a new approach, that combined the optimization of the feeding profiles, the improvement of aeration conditions and the use of a microfiltration bioreactor, was investigated in three different types of fermentation processes. High polysaccharide concentrations (4.73 ± 0.2 g·L-1, with corresponding average yields (0.13 ± 0.006 gK4 CPS·gcdw-1, were obtained; the increase of K4 CPS titre, compared to batch and fed-batch results, was of 16-fold and 3.3-fold respectively, while average yield was almost 3.5 and 1.4 fold higher. Conclusion The increase of capsular polysaccharide titre confirmed the validity of the proposed fermentation strategy and opened the way to the use of the microfiltration bioreactor for the biotechnological production of chondroitin.

  1. Exploring PHD fingers and H3K4me0 interactions with molecular dynamics simulations and binding free energy calculations: AIRE-PHD1, a comparative study.

    Directory of Open Access Journals (Sweden)

    Dimitrios Spiliotopoulos

    Full Text Available PHD fingers represent one of the largest families of epigenetic readers capable of decoding post-translationally modified or unmodified histone H3 tails. Because of their direct involvement in human pathologies they are increasingly considered as a potential therapeutic target. Several PHD/histone-peptide structures have been determined, however relatively little information is available on their dynamics. Studies aiming to characterize the dynamic and energetic determinants driving histone peptide recognition by epigenetic readers would strongly benefit from computational studies. Herein we focus on the dynamic and energetic characterization of the PHD finger subclass specialized in the recognition of histone H3 peptides unmodified in position K4 (H3K4me0. As a case study we focused on the first PHD finger of autoimmune regulator protein (AIRE-PHD1 in complex with H3K4me0. PCA analysis of the covariance matrix of free AIRE-PHD1 highlights the presence of a "flapping" movement, which is blocked in an open conformation upon binding to H3K4me0. Moreover, binding free energy calculations obtained through Molecular Mechanics/Poisson-Boltzmann Surface Area (MM/PBSA methodology are in good qualitative agreement with experiments and allow dissection of the energetic terms associated with native and alanine mutants of AIRE-PHD1/H3K4me0 complexes. MM/PBSA calculations have also been applied to the energetic analysis of other PHD fingers recognizing H3K4me0. In this case we observe excellent correlation between computed and experimental binding free energies. Overall calculations show that H3K4me0 recognition by PHD fingers relies on compensation of the electrostatic and polar solvation energy terms and is stabilized by non-polar interactions.

  2. Infection Exposure Promotes ETV6-RUNX1 Precursor B-cell Leukemia via Impaired H3K4 Demethylases.

    Science.gov (United States)

    Rodríguez-Hernández, Guillermo; Hauer, Julia; Martín-Lorenzo, Alberto; Schäfer, Daniel; Bartenhagen, Christoph; García-Ramírez, Idoia; Auer, Franziska; González-Herrero, Inés; Ruiz-Roca, Lucia; Gombert, Michael; Okpanyi, Vera; Fischer, Ute; Chen, Cai; Dugas, Martin; Bhatia, Sanil; Linka, René Martin; Garcia-Suquia, Marta; Rascón-Trincado, María Victoria; Garcia-Sanchez, Angel; Blanco, Oscar; García-Cenador, Maria Begoña; García-Criado, Francisco Javier; Cobaleda, César; Alonso-López, Diego; De Las Rivas, Javier; Müschen, Markus; Vicente-Dueñas, Carolina; Sánchez-García, Isidro; Borkhardt, Arndt

    2017-08-15

    ETV6-RUNX1 is associated with the most common subtype of childhood leukemia. As few ETV6-RUNX1 carriers develop precursor B-cell acute lymphocytic leukemia (pB-ALL), the underlying genetic basis for development of full-blown leukemia remains to be identified, but the appearance of leukemia cases in time-space clusters keeps infection as a potential causal factor. Here, we present in vivo genetic evidence mechanistically connecting preleukemic ETV6-RUNX1 expression in hematopoetic stem cells/precursor cells (HSC/PC) and postnatal infections for human-like pB-ALL. In our model, ETV6-RUNX1 conferred a low risk of developing pB-ALL after exposure to common pathogens, corroborating the low incidence observed in humans. Murine preleukemic ETV6-RUNX1 pro/preB cells showed high Rag1/2 expression, known for human ETV6-RUNX1 pB-ALL. Murine and human ETV6-RUNX1 pB-ALL revealed recurrent genomic alterations, with a relevant proportion affecting genes of the lysine demethylase (KDM) family. KDM5C loss of function resulted in increased levels of H3K4me3, which coprecipitated with RAG2 in a human cell line model, laying the molecular basis for recombination activity. We conclude that alterations of KDM family members represent a disease-driving mechanism and an explanation for RAG off-target cleavage observed in humans. Our results explain the genetic basis for clonal evolution of an ETV6-RUNX1 preleukemic clone to pB-ALL after infection exposure and offer the possibility of novel therapeutic approaches. Cancer Res; 77(16); 4365-77. ©2017 AACR. ©2017 American Association for Cancer Research.

  3. Sal k 4, a new allergen of Salsola kali, is profilin: a predictive value of conserved conformational regions in cross-reactivity with other plant-derived profilins.

    Science.gov (United States)

    Assarehzadegan, Mohammad Ali; Amini, Akram; Sankian, Mojtaba; Tehrani, Mohsen; Jabbari, Farahzad; Varasteh, Abdolreza

    2010-01-01

    The aim of this study was to investigate a new allergen of Salsola kali, Sal k 4, and to investigate the predictive value of the conserved conformational regions in cross-reactivity with other plant-derived profilins. The Sal k 4-coding sequence was cloned, expressed, and purified by one-step Ni2+ affinity chromatography to recover high-purity target protein. We assessed cross-reactivity and predicted conserved conformational regions among rSal k 4 and other plant-derived profilins. Immunodetection and inhibition assays using 30 individual sera from S. kali allergic patients indicated that purified rSal k 4 might be the same as that in the crude extract. The results of inhibition assays among rSal k 4 and other plant-derived profilins were in accordance with the homology of the predicted conserved conformational regions. Amino acid sequence homology analysis showed that a high degree of IgE cross-reactivity among plant-derived profilins might depend on the predicted conserved conformational regions.

  4. The Histone Demethylase Jarid1b Ensures Faithful Mouse Development by Protecting Developmental Genes from Aberrant H3K4me3

    DEFF Research Database (Denmark)

    Albert, Mareike; Schmitz, Sandra U; Kooistra, Susanne M

    2013-01-01

    of the H3K4me2/3 histone demethylase Jarid1b (Kdm5b/Plu1) results in major neonatal lethality due to respiratory failure. Jarid1b knockout embryos have several neural defects including disorganized cranial nerves, defects in eye development, and increased incidences of exencephaly. Moreover, in line...... with an overlap of Jarid1b and Polycomb target genes, Jarid1b knockout embryos display homeotic skeletal transformations typical for Polycomb mutants, supporting a functional interplay between Polycomb proteins and Jarid1b. To understand how Jarid1b regulates mouse development, we performed a genome-wide analysis...... of histone modifications, which demonstrated that normally inactive genes encoding developmental regulators acquire aberrant H3K4me3 during early embryogenesis in Jarid1b knockout embryos. H3K4me3 accumulates as embryonic development proceeds, leading to increased expression of neural master regulators like...

  5. Altered expression of BRG1 and histone demethylases, and aberrant H3K4 methylation in less developmentally competent embryos at the time of embryonic genome activation.

    Science.gov (United States)

    Glanzner, Werner G; Wachter, Audrey; Coutinho, Ana Rita S; Albornoz, Marcelo S; Duggavathi, Raj; GonÇAlves, Paulo B D; Bordignon, Vilceu

    2017-01-01

    Epigenetics is a fundamental regulator underlying many biological functions, such as development and cell differentiation. Epigenetic modifications affect key chromatin regulation, including transcription and DNA repair, which are critical for normal embryo development. In this study, we profiled the expression of epigenetic modifiers and patterns of epigenetic changes in porcine embryos around the period of embryonic genome activation (EGA). We observed that Brahma-related gene 1 (BRG1) and Lysine demethylase 1A (KDM1A), which can alter the methylation status of lysine 4 in histone 3 (H3K4), localize to the nucleus at Day 3-4 of development. We then compared the abundance of epigenetic modifiers between early- and late-cleaving embryos, which were classified based on the time to the first cell cleavage, to investigate if their nuclear localization contributes to developmental competence. The mRNA abundance of BRG1, KDM1A, as well as other lysine demethylases (KDM1B, KDM5A, KDM5B, and KDM5C), were significantly higher in late- compared to early-cleaving embryos near the EGA period, although these difference disappeared at the blastocyst stage. The abundance of H3K4 mono- (H3K4me) and di-methylation (H3K4me2) during the EGA period was reduced in late-cleaving and less developmentally competent embryos. By contrast, BRG1, KDM1A, and H3K4me2 abundance was greater in embryos with more than eight cells at Day 3-4 of development compared to those with fewer than four cells. These findings suggest that altered epigenetic modifications of H3K4 around the EGA period may affect the developmental capacity of porcine embryos to reach the blastocyst stage. Mol. Reprod. Dev. 84: 19-29, 2017. © 2016 Wiley Periodicals, Inc.

  6. Epigenetic Profiling of H3K4Me3 Reveals Herbal Medicine Jinfukang-Induced Epigenetic Alteration Is Involved in Anti-Lung Cancer Activity.

    Science.gov (United States)

    Lu, Jun; Zhang, Xiaoli; Shen, Tingting; Ma, Chao; Wu, Jun; Kong, Hualei; Tian, Jing; Shao, Zhifeng; Zhao, Xiaodong; Xu, Ling

    2016-01-01

    Traditional Chinese medicine Jinfukang (JFK) has been clinically used for treating lung cancer. To examine whether epigenetic modifications are involved in its anticancer activity, we performed a global profiling analysis of H3K4Me3, an epigenomic marker associated with active gene expression, in JFK-treated lung cancer cells. We identified 11,670 genes with significantly altered status of H3K4Me3 modification following JFK treatment (P JFK. Collectively, these findings provide the first evidence that the anticancer activity of JFK involves modulation of histone modification at many cancer-related gene loci.

  7. Inter-rater reliability of two depression rating scales, MADRS and DRRS, based on videotape records of structured interviews.

    Science.gov (United States)

    Corruble, E; Purper, D; Payan, C; Guelfi, J

    1998-08-01

    The inter-rater reliability of the French versions of the MADRS and the DRRS was studied on the basis of 58 videotape records of structured standardised interviews of depressed inpatients under antidepressant treatment. Each patient was assessed by two trained raters, from the same videotape recording. The inter-rater reliability of total scores was high with both scales (intra-class correlation coefficients: 0.86 for MADRS and 0.77 for DRRS). However, the inter-rater reliability for individual items was higher and more homogeneous for the MADRS than for the DRRS. Finally, the structured interview in French appears to be relevant for the MADRS, but it should be improved for the DRRS.

  8. Chapman's Reef Oculina Banks Clelia Dive 621 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  9. Chapman's Reef Oculina Banks Clelia Dive 620 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  10. Cape Canaveral Oculina Banks Clelia Dive 616 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  11. Sebastian Pinnacles, Oculina Banks Clelia Dive 614 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  12. Jeff's Reef Oculina Banks Clelia Dive 607 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  13. Sebastian Pinnacles, Oculina Banks Clelia Dive 615 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  14. Jeff's Reef Oculina Banks Clelia Dive 606 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  15. Eau Gallie, Oculina Banks Clelia Dive 608 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  16. Eau Galllie Oculina Banks Clelia Dive 609 2001 Digital Imagery - Captured from Videotapes tken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  17. Sebastian Pinnacles Oculina Banks Clelia Dive 618 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  18. Cocoa Beach Oculina Banks Clelia Dive 617 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  19. Sebastian Pinnacles Oculina Banks Clelia Dive 619 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  20. Using case studies and videotaped vignettes to facilitate the development of critical thinking skills in new graduate nurses.

    Science.gov (United States)

    Hooper, Barbara L

    2014-01-01

    Critical thinking skills are an essential component of nursing and crucial to nursing practice. Case studies with videotaped vignettes were used to help facilitate the development of critical thinking skills in new graduate nurses. Results revealed a statistically significant increase (p = .041) on the overall Health Sciences Reasoning Test score. It is essential for educators to be aware of educational strategies that can affect the development of critical thinking skills.

  1. Videotaped patient stories: impact on medical students' attitudes regarding healthcare for the uninsured and underinsured.

    Directory of Open Access Journals (Sweden)

    Richard Bruno

    Full Text Available The attitudes of medical students toward the current United States healthcare system are not well described in the literature. A graded survey was developed to assess awareness and motivation toward the care of the uninsured and underinsured as well as the impact of a video intervention on these attitudes. The survey, which showed good internal consistency (Cronbach's alpha = 0.85, was administered before and after viewing a collection of videotaped patient stories. Although a spectrum of beliefs emerged from the analysis of survey responses, some common attitudes were identified. Eighty-five percent of respondents either agreed or strongly agreed that medical care should be provided to everyone, regardless of their ability to pay. In addition, 66% indicated they would be willing to forgo a portion of their income to provide care to those who do not have access to healthcare services. These values were strongly correlated with increasing respondent age and primary care specialty choice (p<0.01. The video intervention did not heavily influence student responses, perhaps due to a ceiling effect created by the large number of students who were already sympathetic toward the underserved. Overall, this data reflects that United States medical students recognize a need to provide care to the underserved and are willing to make personal sacrifices to meet that need.

  2. Provider Education about Glaucoma and Glaucoma Medications during Videotaped Medical Visits

    Directory of Open Access Journals (Sweden)

    Betsy Sleath

    2014-01-01

    Full Text Available Objective. The purpose of this study was to examine how patient, physician, and situational factors are associated with the extent to which providers educate patients about glaucoma and glaucoma medications, and which patient and provider characteristics are associated with whether providers educate patients about glaucoma and glaucoma medications. Methods. Patients with glaucoma who were newly prescribed or on glaucoma medications were recruited and a cross-sectional study was conducted at six ophthalmology clinics. Patients’ visits were videotape recorded and patients were interviewed after visits. Generalized estimating equations were used to analyze the data. Results. Two hundred and seventy-nine patients participated. Providers were significantly more likely to educate patients about glaucoma and glaucoma medications if they were newly prescribed glaucoma medications. Providers were significantly less likely to educate African American patients about glaucoma. Providers were significantly less likely to educate patients of lower health literacy about glaucoma medications. Conclusion. Eye care providers did not always educate patients about glaucoma or glaucoma medications. Practice Implications. Providers should consider educating more patients about what glaucoma is and how it is treated so that glaucoma patients can better understand their disease. Even if a patient has already been educated once, it is important to reinforce what has been taught before.

  3. [Feeding strategies of mothers of malnourished and eutrophic children: a qualitative study through videotaped observations].

    Science.gov (United States)

    Perosa, Gimol Benzaquen; Carvalhaes, Maria Antonieta de Barros Leite; Benício, Maria Helena D'Aquino; Silveira, Flávia Cristina Pereira

    2011-11-01

    The scope of this study was to identify and compare maternal feeding strategies and characteristics of the interaction between mothers of malnourished and eutrophic children. Eight pairs of mother/malnourished child and eight pairs of mother/eutrophic child (aged between 9 to 24 months) living in poor inner areas, were videotaped during meals, at home. Through analysis of the videos, the strategies were identified and episodes qualitatively analyzed, according to the peculiar characteristics of the interaction, especially maternal responsivity. There were no significant differences in strategies used by the mothers of both groups. The observations of the episodes have shown that feeding a child is a highly interactive process, dependent upon the abilities and characteristics of both partners. The success of feeding appears to be associated with contextual conditions, maternal responsivity and also to the appetite and flexibility of the child. It is suggested that, in projects geared to malnourished children, besides supplements and feeding orientation, special attention be given to maternal self esteem and in helping mothers to deal with children suffering from loss of appetite.

  4. Text-Based Argumentation with Multiple Sources: A Descriptive Study of Opportunity to Learn in Secondary English Language Arts, History, and Science

    Science.gov (United States)

    Litman, Cindy; Marple, Stacy; Greenleaf, Cynthia; Charney-Sirott, Irisa; Bolz, Michael J.; Richardson, Lisa K.; Hall, Allison H.; George, MariAnne; Goldman, Susan R.

    2017-01-01

    This study presents a descriptive analysis of 71 videotaped lessons taught by 34 highly regarded secondary English language arts, history, and science teachers, collected to inform an intervention focused on evidence-based argumentation from multiple text sources. Studying the practices of highly regarded teachers is valuable for identifying…

  5. Text-Based Argumentation with Multiple Sources: A Descriptive Study of Opportunity to Learn in Secondary English Language Arts, History, and Science

    Science.gov (United States)

    Litman, Cindy; Marple, Stacy; Greenleaf, Cynthia; Charney-Sirott, Irisa; Bolz, Michael J.; Richardson, Lisa K.; Hall, Allison H.; George, MariAnne; Goldman, Susan R.

    2017-01-01

    This study presents a descriptive analysis of 71 videotaped lessons taught by 34 highly regarded secondary English language arts, history, and science teachers, collected to inform an intervention focused on evidence-based argumentation from multiple text sources. Studying the practices of highly regarded teachers is valuable for identifying…

  6. An Oral History of First-Generation Leaders in Education of Children with Emotional/Behavioral Disorders, Part 1: The Accidental Special Educator

    Science.gov (United States)

    Kaff, Marilyn S.; Teagarden, James M.; Zabel, Robert H.

    2011-01-01

    As the first part of an oral history of education of students with emotional/behavioral disorders, 15 first-generation leaders were asked to relate how they entered the field and to describe their careers, which span the past 35 to 50 years. Their videotaped responses were transcribed and are reported here together with discussion of several…

  7. 调控因子SlyA和RfaH在E.coli K4合成果糖软骨素中的生理作用%Effects of overexpression of transcriptional regulators SlyA and RfaH on fructosylated chondroitin production in E coli K4

    Institute of Scientific and Technical Information of China (English)

    杨爱华; 刘佳; 张震宇; 刘立明

    2015-01-01

    To enhance K4CPS production of E. coli K4,the transcriptional factor SlyA and RfaH were over expressed in E. coli K4 to create E. coli pTH⁃slyA,E. coli pTH⁃rfaH and co⁃expression strain E. coli pTH⁃slyA⁃rfaH. The over⁃expression of SlyA and RfaH significantly enhanced K4CPS production. The K4CPS concentration for E. coli pTH⁃slyA,E. coli pTH⁃rfaH and E. coli pTH⁃slyA⁃rfaH was increased by 81�8%、46�5% and 153�8%, compared with that of E. coli K4, respectively. The glycerol consumption rate of recombinant strain decreased by 12�5%、18�8% and 31�3% than that of E. coli K4. Moreover, the concentration of acetic acid decreased by 98�6%、39�7%and 91�6%compared with that of E. coli K4� The overexpression of slyA and rfaH could significantly enhanced K4CPS production.%利用基因工程手段过量表达E. coli K4荚膜多糖( K4CPS)合成途径中相关代谢调控因子SlyA和RfaH,以构建高效合成K4CPS的菌株。结果发现:slyA和rfaH基因的单独过量表达和共表达重组菌的K4CPS的产量较对照菌分别提高了81�8%、46�5%和153�8%;甘油比消耗速率较对照菌降低了12�5%、18�8%和31�3%,而乙酸含量则较对照菌降低了98�6%、39�7%和91�6%。因此,调控蛋白SlyA和RfaH的过量表达能够大幅度提高E�coli K4K4CPS合成,并增强菌体对甘油的利用率,而乙酸合成明显降低。而与单独表达策略相比,共表达策略能够更加有效地增强菌体合成K4CPS的能力。

  8. An HST COS 'SNAPSHOT' spectrum of the K supergiant λ Vel (K4Ib-II)

    Energy Technology Data Exchange (ETDEWEB)

    Carpenter, Kenneth G. [NASA/GSFC Code 667, Greenbelt, MD 20771 (United States); Ayres, Thomas R. [University of Colorado, CASA, 389-UCB, Boulder, CO 80309 (United States); Harper, Graham M. [School of Physics, Trinity College, Dublin 2 (Ireland); Kober, Gladys; Nielsen, Krister E.; Wahlgren, Glenn M., E-mail: Kenneth.G.Carpenter@nasa.gov [Deptartment of Physics, Catholic University of America, Washington, DC 20064 (United States)

    2014-10-10

    We present a far-ultraviolet spectrum of the K4 Ib-II supergiant λ Vel obtained with the Hubble Space Telescope's Cosmic Origins Spectrograph (COS) as a part of the SNAPshot program 'SNAPing coronal iron' (GO 11687). The observation covers a wavelength region (1326-1467 Å) not previously recorded for λ Vel at a spectral resolving power of R ∼ 20,000 and displays strong emission and absorption features, superposed on a bright chromospheric continuum. Fluorescent excitation is responsible for much of the observed emission, mainly powered by strong H I Lyα and the O I (UV 2) triplet emission near λ1304. The molecular CO and H{sub 2} fluorescences are weaker than in the early-K giant α Boo while the Fe II and Cr II lines, also pumped by H I Lyα, are stronger in λ Vel. This pattern of relative line strengths between the two stars is explained by the lower iron-group element abundance in α Boo, which weakens that star's Fe II and Cr II emission without reducing the molecular fluorescences. The λ Vel spectrum shows fluorescent Fe II, Cr II, and H{sub 2} emission similar to that observed in the M supergiant α Ori, but more numerous well-defined narrow emissions from CO. The additional CO emissions are visible in the spectrum of λ Vel since that star does not have the cool, opaque circumstellar shells that surround α Ori and produce broad circumstellar CO (A-X) band absorptions that hide those emissions in the cooler star. The presence of Si IV emission in λ Vel indicates a ∼8 × 10{sup 4} K plasma that is mixed into the cooler chromosphere. Evidence of the stellar wind is seen in the C II λλ1334,1335 lines and in the blueshifted Fe II and Ni II wind absorption lines. Line modeling using Sobolev with Exact Integration for the C II lines indicates a larger terminal velocity (∼45 versus ∼30 km s{sup –1}) and turbulence (∼27 versus <21 km s{sup –1}) with a more quickly accelerating wind (β = 0.35 versus 0.7) at the time of

  9. The ATRX-ADD domain binds to H3 tail peptides and reads the combined methylation state of K4 and K9

    Science.gov (United States)

    Dhayalan, Arunkumar; Tamas, Raluca; Bock, Ina; Tattermusch, Anna; Dimitrova, Emilia; Kudithipudi, Srikanth; Ragozin, Sergey; Jeltsch, Albert

    2011-01-01

    Mutations in the ATRX protein are associated with the alpha-thalassemia and mental retardation X-linked syndrome (ATR-X). Almost half of the disease-causing mutations occur in its ATRX-Dnmt3-Dnmt3L (ADD) domain. By employing peptide arrays, chromatin pull-down and peptide binding assays, we show specific binding of the ADD domain to H3 histone tail peptides containing H3K9me3. Peptide binding was disrupted by the presence of the H3K4me3 and H3K4me2 modification marks indicating that the ATRX-ADD domain has a combined readout of these two important marks (absence of H3K4me2 and H3K4me3 and presence of H3K9me3). Disease-causing mutations reduced ATRX-ADD binding to H3 tail peptides. ATRX variants, which fail in the H3K9me3 interaction, show a loss of heterochromatic localization in cells, which indicates the chromatin targeting function of the ADD domain of ATRX. Disruption of H3K9me3 binding may be a general pathogenicity pathway of ATRX mutations in the ADD domain which may explain the clustering of disease mutations in this part of the ATRX protein. PMID:21421568

  10. The ATRX-ADD domain binds to H3 tail peptides and reads the combined methylation state of K4 and K9.

    Science.gov (United States)

    Dhayalan, Arunkumar; Tamas, Raluca; Bock, Ina; Tattermusch, Anna; Dimitrova, Emilia; Kudithipudi, Srikanth; Ragozin, Sergey; Jeltsch, Albert

    2011-06-01

    Mutations in the ATRX protein are associated with the alpha-thalassemia and mental retardation X-linked syndrome (ATR-X). Almost half of the disease-causing mutations occur in its ATRX-Dnmt3-Dnmt3L (ADD) domain. By employing peptide arrays, chromatin pull-down and peptide binding assays, we show specific binding of the ADD domain to H3 histone tail peptides containing H3K9me3. Peptide binding was disrupted by the presence of the H3K4me3 and H3K4me2 modification marks indicating that the ATRX-ADD domain has a combined readout of these two important marks (absence of H3K4me2 and H3K4me3 and presence of H3K9me3). Disease-causing mutations reduced ATRX-ADD binding to H3 tail peptides. ATRX variants, which fail in the H3K9me3 interaction, show a loss of heterochromatic localization in cells, which indicates the chromatin targeting function of the ADD domain of ATRX. Disruption of H3K9me3 binding may be a general pathogenicity pathway of ATRX mutations in the ADD domain which may explain the clustering of disease mutations in this part of the ATRX protein.

  11. The Meiotic Recombination Activator PRDM9 Trimethylates Both H3K36 and H3K4 at Recombination Hotspots In Vivo.

    Science.gov (United States)

    Powers, Natalie R; Parvanov, Emil D; Baker, Christopher L; Walker, Michael; Petkov, Petko M; Paigen, Kenneth

    2016-06-01

    In many mammals, including humans and mice, the zinc finger histone methyltransferase PRDM9 performs the first step in meiotic recombination by specifying the locations of hotspots, the sites of genetic recombination. PRDM9 binds to DNA at hotspots through its zinc finger domain and activates recombination by trimethylating histone H3K4 on adjacent nucleosomes through its PR/SET domain. Recently, the isolated PR/SET domain of PRDM9 was shown capable of also trimethylating H3K36 in vitro, raising the question of whether this reaction occurs in vivo during meiosis, and if so, what its function might be. Here, we show that full-length PRDM9 does trimethylate H3K36 in vivo in mouse spermatocytes. Levels of H3K4me3 and H3K36me3 are highly correlated at hotspots, but mutually exclusive elsewhere. In vitro, we find that although PRDM9 trimethylates H3K36 much more slowly than it does H3K4, PRDM9 is capable of placing both marks on the same histone molecules. In accord with these results, we also show that PRDM9 can trimethylate both K4 and K36 on the same nucleosomes in vivo, but the ratio of K4me3/K36me3 is much higher for the pair of nucleosomes adjacent to the PRDM9 binding site compared to the next pair further away. Importantly, H3K4me3/H3K36me3-double-positive nucleosomes occur only in regions of recombination: hotspots and the pseudoautosomal (PAR) region of the sex chromosomes. These double-positive nucleosomes are dramatically reduced when PRDM9 is absent, showing that this signature is PRDM9-dependent at hotspots; the residual double-positive nucleosomes most likely come from the PRDM9-independent PAR. These results, together with the fact that PRDM9 is the only known mammalian histone methyltransferase with both H3K4 and H3K36 trimethylation activity, suggest that trimethylation of H3K36 plays an important role in the recombination process. Given the known requirement of H3K36me3 for double strand break repair by homologous recombination in somatic cells, we

  12. Community History.

    Science.gov (United States)

    Lewis, Helen M.

    1997-01-01

    Recounts the experience of researching community history in Ivanhoe, Virginia, between 1987 and 1990. The Ivanhoe History Project involved community members in collecting photographs, memorabilia, and oral histories of their town. Subsequent published volumes won the W. D. Weatherford Award and inspired a quilt exhibit and a theatrical production.…

  13. Using video-taped examples of standardized patient to teach medical students taking informed consent

    Directory of Open Access Journals (Sweden)

    SHIRIN HABIBI KHORASANI

    2015-04-01

    Full Text Available Introduction: Medical student should be trained in medical ethics and one of the most essential issues in this field is taking informed consents. In this research, we compared the effect of effectiveness of teaching methods on students’ ability in taking informed consent from patients. Methods: This semi-experimental study was carried out on fifty eight subjects from the 4th-year students of Shiraz University of Medical Sciences who attended in medical ethics course before their ‘clinical clerkship’training.Method of sampling was census and students were randomly allocated into two groups of control group (n=28 was trained in traditional lecture-based class and the case groupnamed as A1 (n=22 were taught by video-taped examples of standardized patient.Then A1 group attended in traditional lecture-based classes named as A2. The groups were evaluated in terms the ability of recognition of ethical issues through the scenario based ethical examination before and after each training. Scenarios were related to the topics of informed consent. Data were analyzed by SPSS 14 software using descriptive statistics and anova test. P-value less than 0.05 was considered as significant. Results: The mean scores results of A2, A1 and B group were found to be 7.21, 5.91 and 5.73 out of 8, respectively. Comparison between the groups demonstrated that the ability of taking informed consent was significantly higher in A2 group (p<0.001, followed by A1 group (p<0.05, while was the least in the B group (p=0.875. Conclusion: According to this research, lecture-based teaching is still of great value in teaching medical ethics, but when combined with standardized patient, the outcome will be much better. It should be considered that mixed methods of teaching should be used together for better result.

  14. MoSET1 (Histone H3K4 Methyltransferase in Magnaporthe oryzae Regulates Global Gene Expression during Infection-Related Morphogenesis.

    Directory of Open Access Journals (Sweden)

    Kieu Thi Minh Pham

    2015-07-01

    Full Text Available Here we report the genetic analyses of histone lysine methyltransferase (KMT genes in the phytopathogenic fungus Magnaporthe oryzae. Eight putative M. oryzae KMT genes were targeted for gene disruption by homologous recombination. Phenotypic assays revealed that the eight KMTs were involved in various infection processes at varying degrees. Moset1 disruptants (Δmoset1 impaired in histone H3 lysine 4 methylation (H3K4me showed the most severe defects in infection-related morphogenesis, including conidiation and appressorium formation. Consequently, Δmoset1 lost pathogenicity on wheat host plants, thus indicating that H3K4me is an important epigenetic mark for infection-related gene expression in M. oryzae. Interestingly, appressorium formation was greatly restored in the Δmoset1 mutants by exogenous addition of cAMP or of the cutin monomer, 16-hydroxypalmitic acid. The Δmoset1 mutants were still infectious on the super-susceptible barley cultivar Nigrate. These results suggested that MoSET1 plays roles in various aspects of infection, including signal perception and overcoming host-specific resistance. However, since Δmoset1 was also impaired in vegetative growth, the impact of MoSET1 on gene regulation was not infection specific. ChIP-seq analysis of H3K4 di- and tri-methylation (H3K4me2/me3 and MoSET1 protein during infection-related morphogenesis, together with RNA-seq analysis of the Δmoset1 mutant, led to the following conclusions: 1 Approximately 5% of M. oryzae genes showed significant changes in H3K4-me2 or -me3 abundance during infection-related morphogenesis. 2 In general, H3K4-me2 and -me3 abundance was positively associated with active transcription. 3 Lack of MoSET1 methyltransferase, however, resulted in up-regulation of a significant portion of the M. oryzae genes in the vegetative mycelia (1,491 genes, and during infection-related morphogenesis (1,385 genes, indicating that MoSET1 has a role in gene repression either

  15. Synthesis of PbS Intercalated K4Nb6O17 Composite and Its Photocatalytic Activity for Hydrogen Production%PbS插层K4Nb6O17复合物的制备及其光催化制氢活性

    Institute of Scientific and Technical Information of China (English)

    崔文权; 刘艳飞; 胡金山; 刘利; 梁英华

    2012-01-01

    采用固相法合成层状半导体K4Nb6O17,通过层间离子交换反应、胺插入反应以及硫化反应制备了纳米PbS插层的K4Nb6O17复合光催化剂(记作K4Nb6O17/PbS).利用X射线衍射(XRD),场发射扫描电镜(SEM),X射线荧光光谱仪(XRF),紫外-可见漫反射(UV-Vis)和分子荧光光谱(PL)等技术对其进行表征.考察了催化剂在Na2SO3和Na2S为牺牲剂的光催化制氢活性.结果表明,PbS的插层拓展了K4Nb6O17对可见光的响应,催化制氢活性也有明显提高.在紫外光和可见光下3h产氢量分别达到123.94和0.66 mmol/(g cat).最后讨论了插层复合催化剂光生电荷转移的机理.%The layered compound K4Nb6O17 was prepared via high temperature solid reaction, and PbS intercalated K4Nb6O17 (designated as K4Nb6O17/PbS) photocatalyst was synthesized via direct ion exchange, alkylamines intercalation and sulfurization procedures. The as-prepared samples were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), ultraviolet-visible diffuse reflection spectra (UV-Vis) and photoluminescence measurement (PL), energy dispersive X-ray detector (EDS) and X-ray fluorescence spectrometer (XRF). The photocatalytic performance of these catalysts for hydrogen production was also investigated in the presence of Na2S and Na2SO3 sacrificial reagents. The absorption edge of K4Nb6O17 shifted to visible light region after the intercalation of PbS. K4Nb6O17/PbS photocatalysts exhibit higher activities for photocatalytic hydrogen production under both UV light and visible light irradiation, and the amounts of hydrogen produced are 123.94 mmol/(g cat) and 0.66 mmol/(g cat) after 3 h irradiation, respectively. The mechanism of charge separation is also discussed.

  16. Entangled histories

    Science.gov (United States)

    Cotler, Jordan; Wilczek, Frank

    2016-12-01

    We introduce quantum history states and their mathematical framework, thereby reinterpreting and extending the consistent histories approach to quantum theory. Through thought experiments, we demonstrate that our formalism allows us to analyze a quantum version of history in which we reconstruct the past by observations. In particular, we can pass from measurements to inferences about ‘what happened’ in a way that is sensible and free of paradox. Our framework allows for a richer understanding of the temporal structure of quantum theory, and we construct history states that embody peculiar, non-classical correlations in time.

  17. Differential expression of K4-AP currents and Kv3.1 potassium channel transcripts in cortical neurons that develop distinct firing phenotypes.

    Science.gov (United States)

    Massengill, J L; Smith, M A; Son, D I; O'Dowd, D K

    1997-05-01

    Maturation of electrical excitability during early postnatal development is critical to formation of functional neural circuitry in the mammalian neocortex. Little is known, however, about the changes in gene expression underlying the development of firing properties that characterize different classes of cortical neurons. Here we describe the development of cortical neurons with two distinct firing phenotypes, regular-spiking (RS) and fast-spiking (FS), that appear to emerge from a population of immature multiple-spiking (IMS) neurons during the first two postnatal weeks, both in vivo (within layer IV) and in vitro. We report the expression of a slowly inactivating, 4-AP-sensitive potassium current (K4-AP) at significantly higher density in FS compared with RS neurons. The same current is expressed at intermediate levels in IMS neurons. The kinetic, voltage-dependent, and pharmacological properties of the K4-AP current are similar to those observed by heterologous expression of Kv3.1 potassium channel mRNA. Single-cell RT-PCR analysis demonstrates that PCR products representing Kv3.1 transcripts are amplified more frequently from FS than RS neurons, with an intermediate frequency of Kv3.1 detection in neurons with immature firing properties. Taken together, these data suggest that the Kv3.1 gene encodes the K4-AP current and that expression of this gene is regulated in a cell-specific manner during development. Analysis of the effects of 4-AP on firing properties suggests that the K4-AP current is important for rapid action potential repolarization, fast after-hyperpolarization, brief refractory period, and high firing frequency characteristic of FS GABAergic interneurons.

  18. Histone H3 Acetyl K9 and Histone H3 Tri Methyl K4 as Prognostic Markers for Patients with Cervical Cancer

    Directory of Open Access Journals (Sweden)

    Susanne Beyer

    2017-02-01

    Full Text Available Chromatin remodeling alters gene expression in carcinoma tissue. Although cervical cancer is the fourth most common cancer in women worldwide, a systematic study about the prognostic value of specific changes in the chromatin structure, such as histone acetylation or histone methylation, is missing. In this study, the expression of histone H3 acetyl K9, which is known to denote active regions at enhancers and promoters, and histone H3 tri methyl K4, which preferentially identifies active gene promoters, were examined as both show high metastatic potential. A panel of patients with cervical cancer was selected and the importance of the histone modifications concerning survival-time (overall survival and relapse-free survival was analyzed in 250 cases. Histone H3 acetyl K9 staining was correlated with low grading, low FIGO (TNM classification and the International Federation of Gynecology and Obstetrics status, negative N-status and low T-status in cervical cancer, showing a higher expression in adenocarcinoma than in squamous cell carcinoma. Cytoplasmic expression of histone H3 tri methyl K4 in a cervical cancer specimen was correlated with advanced T-status and poor prognosis. While cytoplasmic H3K4me3 expression seemed to be a marker of relapse-free survival, nuclear expression showed a correlation to poor prognosis in overall survival. Within this study, we analyzed the chemical modification of two histone proteins that are connected to active gene expression. Histone H3 acetyl K9 was found to be an independent marker of overall survival. Histone H3 tri methyl K4 was correlated with poor prognosis and it was found to be an independent marker of relapse-free survival. Therefore, we could show that chromatin remodeling plays an important role in cervical cancer biology.

  19. Histone H3 Acetyl K9 and Histone H3 Tri Methyl K4 as Prognostic Markers for Patients with Cervical Cancer.

    Science.gov (United States)

    Beyer, Susanne; Zhu, Junyan; Mayr, Doris; Kuhn, Christina; Schulze, Sandra; Hofmann, Simone; Dannecker, Christian; Jeschke, Udo; Kost, Bernd P

    2017-02-23

    Chromatin remodeling alters gene expression in carcinoma tissue. Although cervical cancer is the fourth most common cancer in women worldwide, a systematic study about the prognostic value of specific changes in the chromatin structure, such as histone acetylation or histone methylation, is missing. In this study, the expression of histone H3 acetyl K9, which is known to denote active regions at enhancers and promoters, and histone H3 tri methyl K4, which preferentially identifies active gene promoters, were examined as both show high metastatic potential. A panel of patients with cervical cancer was selected and the importance of the histone modifications concerning survival-time (overall survival and relapse-free survival) was analyzed in 250 cases. Histone H3 acetyl K9 staining was correlated with low grading, low FIGO (TNM classification and the International Federation of Gynecology and Obstetrics) status, negative N-status and low T-status in cervical cancer, showing a higher expression in adenocarcinoma than in squamous cell carcinoma. Cytoplasmic expression of histone H3 tri methyl K4 in a cervical cancer specimen was correlated with advanced T-status and poor prognosis. While cytoplasmic H3K4me3 expression seemed to be a marker of relapse-free survival, nuclear expression showed a correlation to poor prognosis in overall survival. Within this study, we analyzed the chemical modification of two histone proteins that are connected to active gene expression. Histone H3 acetyl K9 was found to be an independent marker of overall survival. Histone H3 tri methyl K4 was correlated with poor prognosis and it was found to be an independent marker of relapse-free survival. Therefore, we could show that chromatin remodeling plays an important role in cervical cancer biology.

  20. Inflammatory genes TNFα and IL6 display no signs of increased H3K4me3 in circulating monocytes from untreated rheumatoid arthritis patients.

    Science.gov (United States)

    Messemaker, T C; Mikkers, H M M; Huizinga, T W; Toes, R E M; van der Helm-van Mil, A H M; Kurreeman, F

    2017-09-01

    Innate immune cells, such as monocytes, can adopt a long-lasting pro-inflammatory phenotype, a phenomenon called 'trained immunity'. In trained immunity, increased cytokine levels of genes, like interleukin (IL)-6 and tumor necrosis factor (TNF)-α, are observed, which are associated with increased histone 3 lysine 4 trimethylation (H3K4me3) in the promoter region. As systemic IL6 and TNFα levels are increased in rheumatoid arthritis (RA) patients and monocytes are known to be the primary producers of TNFα and IL6, we hypothesized that 'trained immunity' signals may be observed at these genes in monocytes from RA patients. CD14+ monocytes were isolated from untreated RA patients and paired age-matched healthy controls. H3K4me3, mRNA, protein and serum levels of IL6 and TNFα were evaluated by chromatin immunoprecipitation, reverse-transcription quantitative PCR and enzyme-linked immunosorbent assays. Despite elevated serum levels of TNFα and IL6 in the tested RA patients (P<0.05), ex vivo isolated monocytes displayed similar H3K4me3 levels to healthy controls in the promoter region of TNFα and IL6. Concordantly, mRNA and protein levels of IL6 and TNFα were similar before and after lipopolysaccharide stimulation between patients and controls. Together, with the current number of individuals tested we have not detected enhanced trained immunity signals in circulating monocytes from untreated RA patients, despite increased IL6 and TNFα serum levels.

  1. Pro Isomerization in MLL1 PHD3-Bromo Cassette Connects H3K4me Readout to CyP33 and HDAC-Mediated Repression

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Zhanxin; Song, Jikui; Milne, Thomas A.; Wang, Gang G.; Li, Haitao; Allis, C. David; Patel, Dinshaw J. (MSKCC); (Rockefeller)

    2010-09-13

    The MLL1 gene is a frequent target for recurrent chromosomal translocations, resulting in transformation of hematopoietic precursors into leukemia stem cells. Here, we report on structure-function studies that elucidate molecular events in MLL1 binding of histone H3K4me3/2 marks and recruitment of the cyclophilin CyP33. CyP33 contains a PPIase and a RRM domain and regulates MLL1 function through HDAC recruitment. We find that the PPIase domain of CyP33 regulates the conformation of MLL1 through proline isomerization within the PHD3-Bromo linker, thereby disrupting the PHD3-Bromo interface and facilitating binding of the MLL1-PHD3 domain to the CyP33-RRM domain. H3K4me3/2 and CyP33-RRM target different surfaces of MLL1-PHD3 and can bind simultaneously to form a ternary complex. Furthermore, the MLL1-CyP33 interaction is required for repression of HOXA9 and HOXC8 genes in vivo. Our results highlight the role of PHD3-Bromo cassette as a regulatory platform, orchestrating MLL1 binding of H3K4me3/2 marks and cyclophilin-mediated repression through HDAC recruitment.

  2. The Demethylase JMJD2C Localizes to H3K4me3 Positive Transcription Start Sites and Is Dispensable for Embryonic Development

    DEFF Research Database (Denmark)

    Pedersen, Marianne Terndrup; Agger, Karl; Laugesen, Anne

    2014-01-01

    cell (ESC) self-renewal and embryonic development. Moreover, we report that JMJD2C localizes to H3K4me3 positive transcription start sites in both primary cells and in the human carcinoma KYSE150 cell line, containing an amplification of the JMJD2C locus. Binding is dependent on the double Tudor domain...... of JMJD2C, which recognizes H3K4me3, but not H4K20me2/me3 in vitro, thus showing a different binding specificity than the double Tudor domains of JMJD2A and JMJD2B. Depletion of JMJD2C in KYSE150 cells has modest impact on H3K9me3 and H3K36me3 levels, but impairs proliferation and leads to deregulated...... expression of a subset of target genes involved in cell cycle progression. Taken together, we show that JMJD2C is targeted to H3K4me3 positive transcription start sites, where it can contribute to transcriptional regulation, and report that the putative oncogene, JMJD2C, is not generally required...

  3. Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axes.

    Science.gov (United States)

    Sommermeyer, Vérane; Béneut, Claire; Chaplais, Emmanuel; Serrentino, Maria Elisabetta; Borde, Valérie

    2013-01-10

    Meiotic chromosomes are organized into arrays of loops that are anchored to the chromosome axis structure. Programmed DNA double-strand breaks (DSBs) that initiate meiotic recombination, catalyzed by Spo11 and accessory DSB proteins, form in loop sequences in promoters, whereas the DSB proteins are located on chromosome axes. Mechanisms bridging these two chromosomal regions for DSB formation have remained elusive. Here we show that Spp1, a conserved member of the histone H3K4 methyltransferase Set1 complex, is required for normal levels of DSB formation and is associated with chromosome axes during meiosis, where it physically interacts with the Mer2 DSB protein. The PHD finger module of Spp1, which reads H3K4 methylation close to promoters, promotes DSB formation by tethering these regions to chromosome axes and activating cleavage by the DSB proteins. This paper provides the molecular mechanism linking DSB sequences to chromosome axes and explains why H3K4 methylation is important for meiotic recombination.

  4. The histone-H3K4-specific demethylase KDM5B binds to its substrate and product through distinct PHD fingers.

    Science.gov (United States)

    Klein, Brianna J; Piao, Lianhua; Xi, Yuanxin; Rincon-Arano, Hector; Rothbart, Scott B; Peng, Danni; Wen, Hong; Larson, Connie; Zhang, Xi; Zheng, Xia; Cortazar, Michael A; Peña, Pedro V; Mangan, Anthony; Bentley, David L; Strahl, Brian D; Groudine, Mark; Li, Wei; Shi, Xiaobing; Kutateladze, Tatiana G

    2014-01-30

    The histone lysine demethylase KDM5B regulates gene transcription and cell differentiation and is implicated in carcinogenesis. It contains multiple conserved chromatin-associated domains, including three PHD fingers of unknown function. Here, we show that the first and third, but not the second, PHD fingers of KDM5B possess histone binding activities. The PHD1 finger is highly specific for unmodified histone H3 (H3K4me0), whereas the PHD3 finger shows preference for the trimethylated histone mark H3K4me3. RNA-seq analysis indicates that KDM5B functions as a transcriptional repressor for genes involved in inflammatory responses, cell proliferation, adhesion, and migration. Biochemical analysis reveals that KDM5B associates with components of the nucleosome remodeling and deacetylase (NuRD) complex and may cooperate with the histone deacetylase 1 (HDAC1) in gene repression. KDM5B is downregulated in triple-negative breast cancer relative to estrogen-receptor-positive breast cancer. Overexpression of KDM5B in the MDA-MB 231 breast cancer cells suppresses cell migration and invasion, and the PHD1-H3K4me0 interaction is essential for inhibiting migration. These findings highlight tumor-suppressive functions of KDM5B in triple-negative breast cancer cells and suggest a multivalent mechanism for KDM5B-mediated transcriptional regulation. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

  5. The Histone-H3K4-Specific Demethylase KDM5B Binds to Its Substrate and Product through Distinct PHD Fingers

    Directory of Open Access Journals (Sweden)

    Brianna J. Klein

    2014-01-01

    Full Text Available The histone lysine demethylase KDM5B regulates gene transcription and cell differentiation and is implicated in carcinogenesis. It contains multiple conserved chromatin-associated domains, including three PHD fingers of unknown function. Here, we show that the first and third, but not the second, PHD fingers of KDM5B possess histone binding activities. The PHD1 finger is highly specific for unmodified histone H3 (H3K4me0, whereas the PHD3 finger shows preference for the trimethylated histone mark H3K4me3. RNA-seq analysis indicates that KDM5B functions as a transcriptional repressor for genes involved in inflammatory responses, cell proliferation, adhesion, and migration. Biochemical analysis reveals that KDM5B associates with components of the nucleosome remodeling and deacetylase (NuRD complex and may cooperate with the histone deacetylase 1 (HDAC1 in gene repression. KDM5B is downregulated in triple-negative breast cancer relative to estrogen-receptor-positive breast cancer. Overexpression of KDM5B in the MDA-MB 231 breast cancer cells suppresses cell migration and invasion, and the PHD1-H3K4me0 interaction is essential for inhibiting migration. These findings highlight tumor-suppressive functions of KDM5B in triple-negative breast cancer cells and suggest a multivalent mechanism for KDM5B-mediated transcriptional regulation.

  6. Epigenetic Profiling of H3K4Me3 Reveals Herbal Medicine Jinfukang-Induced Epigenetic Alteration Is Involved in Anti-Lung Cancer Activity

    Directory of Open Access Journals (Sweden)

    Jun Lu

    2016-01-01

    Full Text Available Traditional Chinese medicine Jinfukang (JFK has been clinically used for treating lung cancer. To examine whether epigenetic modifications are involved in its anticancer activity, we performed a global profiling analysis of H3K4Me3, an epigenomic marker associated with active gene expression, in JFK-treated lung cancer cells. We identified 11,670 genes with significantly altered status of H3K4Me3 modification following JFK treatment (P<0.05. Gene Ontology analysis indicates that these genes are involved in tumor-related pathways, including pathway in cancer, basal cell carcinoma, apoptosis, induction of programmed cell death, regulation of transcription (DNA-templated, intracellular signal transduction, and regulation of peptidase activity. In particular, we found that the levels of H3K4Me3 at the promoters of SUSD2, CCND2, BCL2A1, and TMEM158 are significantly altered in A549, NCI-H1975, NCI-H1650, and NCI-H2228 cells, when treated with JFK. Collectively, these findings provide the first evidence that the anticancer activity of JFK involves modulation of histone modification at many cancer-related gene loci.

  7. 油水自组装制备Ag@AgCl/K4Nb6 O17及其光催化降解有机污染物的研究∗%Oil-water self-assembly synthesis of Ag@AgCl/K4 Nb6 O17 and enhanced photocatalytic degradation of organic pollutants

    Institute of Scientific and Technical Information of China (English)

    赵俊秀; 刘利; 胡金山; 梁英华; 崔文权

    2015-01-01

    在高温固相法制备K4 Nb6 O17的基础上,采用油水自组装合成复合光催化剂 Ag @ AgCl/K4 Nb6 O17,利用 XRD、SEM、TEM、EDX、UV-Vis、PL、BET等多种手段对复合光催化剂的微观结构、形貌和性能进行了表征,并研究了可见光下降解亚甲基蓝(MB)的催化性能.研究表明,Ag@AgCl 的粒径约为20 nm,均匀分散在二维层状结构K4 Nb6 O17的表面上;贵金属Ag纳米粒子的表面等离子体效应显著,对可见光的吸收范围从400 nm 拓展到800 nm;Ag@AgCl(25%(质量分数))/K4Nb6O17在可见光照射60 min对亚甲基蓝的降解率为88.2%,远远高于单体Ag@AgCl和K4 Nb6 O17的活性.循环实验证明催化剂具有较好的稳定性,同时对苯酚和罗丹明 B(RhB)也具有一定的催化降解活性.淬灭实验表明自由基和空穴均为活性物种,并在此基础上提出降解机理.%The composite photocatalyst of Ag@AgCl/K4 Nb6 O17 was composed of by the oil-water self-assembly method,on the basis that K4 Nb6 O17 was prepared in the high-temperature solid-state method.The microstruc-ture,morphology and properties of Ag@AgCl/K4 Nb6 O17 composite photocatalyst were characterized by means of XRD,SEM,TEM,EDX,UV-vis,PL,BET;and the photocatalytic performance was valued by the degra-dation of methylene blue (MB),phenol,and Rhodamine B (RhB)under visible light irradiation.The results shows that Ag@AgCl particles with the size of about 20 nm were dispersed in two-dimensional layer structure on the surface of K4 Nb6 O17 and the absorption of visible light of the composite photocatalyst ranged from 400 to 800 nm due to the surface plasmon resonance of Ag.The degradation rate of MB solution over Ag@AgCl (25wt%)/K4 Nb6 O17 under the visible light irradiation reaches 88.2% in an hour,which is more than two times compared with the sum of the Ag@AgCl and K4 Nb6 O17 degradation rate.The composite photocatalyst still ex-hibited high catalytic activity after recycled five times

  8. Intellectual History

    DEFF Research Database (Denmark)

    In the 5 Questions book series, this volume presents a range of leading scholars in Intellectual History and the History of Ideas through their answers to a brief questionnaire. Respondents include Michael Friedman, Jacques le Goff, Hans Ulrich Gumbrecht, Jonathan Israel, Phiip Pettit, John Pocock...

  9. Intellectual History

    DEFF Research Database (Denmark)

    In the 5 Questions book series, this volume presents a range of leading scholars in Intellectual History and the History of Ideas through their answers to a brief questionnaire. Respondents include Michael Friedman, Jacques le Goff, Hans Ulrich Gumbrecht, Jonathan Israel, Phiip Pettit, John Pocock...

  10. Romerrigets historie

    DEFF Research Database (Denmark)

    Christiansen, Erik

    Romerrigets historie fra Roms legendariske grundlæggelse i 753 f.v.t. til Heraklios' tronbestigelse i 610 e.v.t.......Romerrigets historie fra Roms legendariske grundlæggelse i 753 f.v.t. til Heraklios' tronbestigelse i 610 e.v.t....

  11. Stage and gene specific signatures defined by histones H3K4me2 and H3K27me3 accompany mammalian retina maturation in vivo.

    Science.gov (United States)

    Popova, Evgenya Y; Xu, Xuming; DeWan, Andrew T; Salzberg, Anna C; Berg, Arthur; Hoh, Josephine; Zhang, Samuel S; Barnstable, Colin J

    2012-01-01

    The epigenetic contribution to neurogenesis is largely unknown. There is, however, growing evidence that posttranslational modification of histones is a dynamic process that shows many correlations with gene expression. Here we have followed the genome-wide distribution of two important histone H3 modifications, H3K4me2 and H3K27me3 during late mouse retina development. The retina provides an ideal model for these studies because of its well-characterized structure and development and also the extensive studies of the retinal transcriptome and its development. We found that a group of genes expressed only in mature rod photoreceptors have a unique signature consisting of de-novo accumulation of H3K4me2, both at the transcription start site (TSS) and over the whole gene, that correlates with the increase in transcription, but no accumulation of H3K27me3 at any stage. By in silico analysis of this unique signature we have identified a larger group of genes that may be selectively expressed in mature rod photoreceptors. We also found that the distribution of H3K4me2 and H3K27me3 on the genes widely expressed is not always associated with their transcriptional levels. Different histone signatures for retinal genes with the same gene expression pattern suggest the diversities of epigenetic regulation. Genes without H3K4me2 and H3K27me3 accumulation at any stage represent a large group of transcripts never expressed in retina. The epigenetic signatures defined by H3K4me2 and H3K27me3 can distinguish cell-type specific genes from widespread transcripts and may be reflective of cell specificity during retina maturation. In addition to the developmental patterns seen in wild type retina, the dramatic changes of histone modification in the retinas of mutant animals lacking rod photoreceptors provide a tool to study the epigenetic changes in other cell types and thus describe a broad range of epigenetic events in a solid tissue in vivo.

  12. Stage and gene specific signatures defined by histones H3K4me2 and H3K27me3 accompany mammalian retina maturation in vivo.

    Directory of Open Access Journals (Sweden)

    Evgenya Y Popova

    Full Text Available The epigenetic contribution to neurogenesis is largely unknown. There is, however, growing evidence that posttranslational modification of histones is a dynamic process that shows many correlations with gene expression. Here we have followed the genome-wide distribution of two important histone H3 modifications, H3K4me2 and H3K27me3 during late mouse retina development. The retina provides an ideal model for these studies because of its well-characterized structure and development and also the extensive studies of the retinal transcriptome and its development. We found that a group of genes expressed only in mature rod photoreceptors have a unique signature consisting of de-novo accumulation of H3K4me2, both at the transcription start site (TSS and over the whole gene, that correlates with the increase in transcription, but no accumulation of H3K27me3 at any stage. By in silico analysis of this unique signature we have identified a larger group of genes that may be selectively expressed in mature rod photoreceptors. We also found that the distribution of H3K4me2 and H3K27me3 on the genes widely expressed is not always associated with their transcriptional levels. Different histone signatures for retinal genes with the same gene expression pattern suggest the diversities of epigenetic regulation. Genes without H3K4me2 and H3K27me3 accumulation at any stage represent a large group of transcripts never expressed in retina. The epigenetic signatures defined by H3K4me2 and H3K27me3 can distinguish cell-type specific genes from widespread transcripts and may be reflective of cell specificity during retina maturation. In addition to the developmental patterns seen in wild type retina, the dramatic changes of histone modification in the retinas of mutant animals lacking rod photoreceptors provide a tool to study the epigenetic changes in other cell types and thus describe a broad range of epigenetic events in a solid tissue in vivo.

  13. Quantum Histories

    CERN Document Server

    Kent, A

    1998-01-01

    There are good motivations for considering some type of quantum histories formalism. Several possible formalisms are known, defined by different definitions of event and by different selection criteria for sets of histories. These formalisms have a natural interpretation, according to which nature somehow chooses one set of histories from among those allowed, and then randomly chooses to realise one history from that set; other interpretations are possible, but their scientific implications are essentially the same. The selection criteria proposed to date are reasonably natural, and certainly raise new questions. For example, the validity of ordering inferences which we normally take for granted --- such as that a particle in one region is necessarily in a larger region containing it --- depends on whether or not our history respects the criterion of ordered consistency, or merely consistency. However, the known selection criteria, including consistency and medium decoherence, are very weak. It is not possibl...

  14. Evaluation of two videotape instruction programmes on how to break bad news--for Cantonese-speaking medical students in Hong Kong.

    Science.gov (United States)

    Betson, C L; Fielding, R; Wong, G; Chung, S F; Nestel, D F

    1997-12-01

    To evaluate a culture-specific videotape on how to 'break bad news' and another videotape produced by a western university, and to determine if the language of presentation influenced the students' perceived abilities to execute basic skills. Third year medical students at the Faculty of Medicine, the University of Hong Kong. Longitudinal study with experimental design. Two instructional tapes on breaking bad news; one using Chinese speaking role models and one using English. In both groups, self-efficacy summed scores increased from 26.8 (95% CI = 25.9-27.7) at the pre-test to 29.0 (95% CI = 28.4-29.6). The biggest changes occurred in perceived self-efficacy regarding specific skills. However, students using the Chinese tape rated skills as more useful than those using the English tape. The videotapes were useful in teaching communication skills. Culturally relevant audiovisual materials were more effective.

  15. Family History

    Science.gov (United States)

    Your family history includes health information about you and your close relatives. Families have many factors in common, including their genes, ... as heart disease, stroke, and cancer. Having a family member with a disease raises your risk, but ...

  16. The Application of Red Pigments from Streptomyces K-4B and Dayak Onions (Eleutherine palmifolia (L.) Merr.) In Colouring Glycerine Soap

    Science.gov (United States)

    Herlina; Asnani, A.; Diastuti, H.

    2017-02-01

    Glycerin soap has been colored with red pigment from Streptomyces K-4 B and Dayak onion (Eleutherine palmifolia (L.) Merr). Both red pigments from Streptomyces K-4B and Dayak onion were extracted with ethanol by maceration method, followed with soxhlet extraction. The concentration of red pigment added was varied (0, 200, 300, 400 μL) to evaluate the best product. The resulted glycerine soaps were characterized and analyzed based on SNI 06-3532-1994. The research results indicated that the glycerine soap has water content ranged from 0.36% to 12.56%; the amount of fatty acid ranged from 14% to 36.75%; the amount of free fatty acids ranged from 0% to 0.37%; the non-saponifiable fat ranged from 0.001 to 0.019%; the pH ranged from 10.33 to 11.06; the foam stability ranged from 0.61% to 89.09%. The results of analysis of variance showed that the effect between treatments significantly different at 95% confidence level (α = 0.05) on the characteristics of glycerine soap. The results of an organoleptic test with parameters observed were color, aroma, texture, foam, rough impression upon usage and rough impression after usage, gave “like to very like soap” with a maximum score of 4.67 (1 to 5 scale). Based on the color assessment, the organoleptic panelists preferred the glycerine soap of SK-4B3 (red pigment from Streptomyces K-4B, 200 μL) with the score of 4.30 (like to very like).

  17. Ag 插层 K4Nb6O17复合光催化剂降解水中有机污染物%The Degradation of Organic Pollutants in Water on The Ag-intercalated K4Nb6O17 Composite Photocatalyst

    Institute of Scientific and Technical Information of China (English)

    穆晨帆; 梁英华; 刘利; 胡金山; 崔文权

    2015-01-01

    Ag-intercalated layered niobate (denoted as K4Nb6O17/Ag) was synthesized via a micr-owave-assisted ion-exchange method. The high enhanced activity of the composite photocatalyst was observed during the degradation of methylene blue (MB). Also, the degradation rate of MB by K4Nb6O17/Ag was higher when the solution pH increased. The composite photocatalyst showed the high enhanced activity with the different concentration of MB. In the same condition, K4Nb6O17/Ag showed higher photocatalysis than Ag-loaded K4Nb6O17 and Ag-loaded TiO2 composite photocat-alysts.%介绍了通过微波辅助的离子交换法制备 Ag 插层 K4Nb6O17复合光催化剂。复合光催化剂在降解水体中亚甲基蓝的过程中呈现出很高的光催化活性,并且插层复合光催化剂对亚基蓝在碱性条件下能发挥更高效的降解作用且对不同初始浓度的亚甲基蓝均具有较好的移除降解效率。在相同的反应条件下, Ag 插层复合光催化剂要比等量的 Ag 负载 K4Nb6O17, Ag 负载 TiO2复合光催化剂活性高很多,使 Ag 插层 K4Nb6O17复合光催化剂在净化水体污染物方面表现出更显著的优势。

  18. Re-patterning of H3K27me3, H3K4me3 and DNA methylation during fibroblast conversion into induced cardiomyocytes

    Directory of Open Access Journals (Sweden)

    Ziqing Liu

    2016-03-01

    Full Text Available Direct conversion of fibroblasts into induced cardiomyocytes (iCMs offers an alternative strategy for cardiac disease modeling and regeneration. During iCM reprogramming, the starting fibroblasts must overcome existing epigenetic barriers to acquire the CM-like chromatin pattern. However, epigenetic dynamics along this reprogramming process have not been studied. Here, we took advantage of our recently generated polycistronic system and determined the dynamics of two critical histone marks, H3K27me3 and H3K4me3, in parallel with gene expression at a set of carefully selected cardiac and fibroblast loci during iCM reprogramming. We observed reduced H3K27me3 and increased H3K4me3 at cardiac promoters as early as day 3, paralleled by a rapid significant increase in their mRNA expression. In contrast, H3K27me3 at loci encoding fibroblast marker genes did not increase until day 10 and H3K4me3 progressively decreased along the reprogramming process; these changes were accompanied by a gradual decrease in the mRNA expression of fibroblast marker genes. Further analyses of fibroblast-enriched transcription factors revealed a similarly late deposition of H3K27me3 and decreased mRNA expression of Sox9, Twist1 and Twist2, three important players in epithelial−mesenchymal transition. Our data suggest early rapid activation of the cardiac program and later progressive suppression of fibroblast fate at both epigenetic and transcriptional levels. Additionally, we determined the DNA methylation states of representative cardiac promoters and found that not every single CpG was equally demethylated during early stages of iCM reprogramming. Rather, there are specific CpGs, whose demethylation states correlated tightly with transcription activation, that we propose are the major contributing CpGs. Our work thus reveals a differential re-patterning of H3K27me3, H3K4me3 at cardiac and fibroblast loci during iCM reprogramming and could provide future genome

  19. Electrochemical studies on Li+/K+ ion exchange behaviour in K4Fe(CN)6 cathode material for Li, K-ion battery

    Indian Academy of Sciences (India)

    Bikash Mandal; I Basumallick; Susanta Ghosha

    2015-01-01

    The electrochemical studies of anhydrous K4Fe(CN)6 is reported. Anhydrous material was produced after dehydrating K4Fe(CN)6.3H2O crystal at 200°C in an open atmosphere. The material, as obtained, was characterized by various spectroscopic techniques, such as UV-Visible, FTIR, powder X-ray diffraction and FESEM-EDX. Electrochemical and Li+/K+ ion exchange behaviour of the synthesized material were studied by cyclic voltametry (CV), chronoamperometry (CA) and galvanostatic charge-discharge method after preparing a laboratory model cell against lithium anode instead of potassium. During anodic scan in the 1st cycle, peak maximum was observed at 3.93 V vs. Li+/Li due to removal of K+ ions from the ferrocyanide matrix, whereas, in the reverse scan (cathodic sweep) as well as in consequent cycles, peak maxima due to Li+ ion insertion and extraction were observed at 2.46 V and 3.23 V vs. Li+/Li, respectively. Cell, assembled using ferrocyanide cathode and lithium anode, shows an open circuit potential of 3.08 V and delivers a maximum capacity of 61 mAh g-1 (theoretical capacity 72 mAh g-1) at a rate of 0.2 C at room temperature.

  20. The inflammatory kinase MAP4K4 promotes reactivation of Kaposi's sarcoma herpesvirus and enhances the invasiveness of infected endothelial cells.

    Directory of Open Access Journals (Sweden)

    Darya A Haas

    Full Text Available Kaposi's sarcoma (KS is a mesenchymal tumour, which is caused by Kaposi's sarcoma herpesvirus (KSHV and develops under inflammatory conditions. KSHV-infected endothelial spindle cells, the neoplastic cells in KS, show increased invasiveness, attributed to the elevated expression of metalloproteinases (MMPs and cyclooxygenase-2 (COX-2. The majority of these spindle cells harbour latent KSHV genomes, while a minority undergoes lytic reactivation with subsequent production of new virions and viral or cellular chemo- and cytokines, which may promote tumour invasion and dissemination. In order to better understand KSHV pathogenesis, we investigated cellular mechanisms underlying the lytic reactivation of KSHV. Using a combination of small molecule library screening and siRNA silencing we found a STE20 kinase family member, MAP4K4, to be involved in KSHV reactivation from latency and to contribute to the invasive phenotype of KSHV-infected endothelial cells by regulating COX-2, MMP-7, and MMP-13 expression. This kinase is also highly expressed in KS spindle cells in vivo. These findings suggest that MAP4K4, a known mediator of inflammation, is involved in KS aetiology by regulating KSHV lytic reactivation, expression of MMPs and COX-2, and, thereby modulating invasiveness of KSHV-infected endothelial cells.

  1. Signalling couples hair follicle stem cell quiescence with reduced histone H3 K4/K9/K27me3 for proper tissue homeostasis.

    Science.gov (United States)

    Lee, Jayhun; Kang, Sangjo; Lilja, Karin C; Colletier, Keegan J; Scheitz, Cornelia Johanna Franziska; Zhang, Ying V; Tumbar, Tudorita

    2016-04-15

    Mechanisms of plasticity to acquire different cell fates are critical for adult stem cell (SC) potential, yet are poorly understood. Reduced global histone methylation is an epigenetic state known to mediate plasticity in cultured embryonic SCs and T-cell progenitors. Here we find histone H3 K4/K9/K27me3 levels actively reduced in adult mouse skin and hair follicle stem cells (HFSCs) during G0 quiescence. The level of marks over specific gene promoters did not correlate to mRNA level changes in quiescent HFSCs. Skin hypomethylation during quiescence was necessary for subsequent progression of hair homeostasis (cycle). Inhibiting BMP signal, a known HFSC anti-proliferative factor, elevated HFSC methylation in vivo during quiescence prior to proliferation onset. Furthermore, removal of proliferation factors and addition of BMP4 reduced histone methylases and increased demethylases mRNAs in cultured skin epithelial cells. We conclude that signalling couples hair follicle stem cell quiescence with reduced H3 K4/K9/K27me3 levels for proper tissue homeostasis.

  2. H3K4me3 demethylation by the histone demethylase KDM5C/JARID1C promotes DNA replication origin firing.

    Science.gov (United States)

    Rondinelli, Beatrice; Schwerer, Hélène; Antonini, Elena; Gaviraghi, Marco; Lupi, Alessio; Frenquelli, Michela; Cittaro, Davide; Segalla, Simona; Lemaitre, Jean-Marc; Tonon, Giovanni

    2015-03-11

    DNA replication is a tightly regulated process that initiates from multiple replication origins and leads to the faithful transmission of the genetic material. For proper DNA replication, the chromatin surrounding origins needs to be remodeled. However, remarkably little is known on which epigenetic changes are required to allow the firing of replication origins. Here, we show that the histone demethylase KDM5C/JARID1C is required for proper DNA replication at early origins. JARID1C dictates the assembly of the pre-initiation complex, driving the binding to chromatin of the pre-initiation proteins CDC45 and PCNA, through the demethylation of the histone mark H3K4me3. Fork activation and histone H4 acetylation, additional early events involved in DNA replication, are not affected by JARID1C downregulation. All together, these data point to a prominent role for JARID1C in a specific phase of DNA replication in mammalian cells, through its demethylase activity on H3K4me3.

  3. Distinct localization of histone H3 acetylation and H3-K4 methylation to the transcription start sites in the human genome

    Science.gov (United States)

    Liang, Gangning; Lin, Joy C. Y.; Wei, Vivian; Yoo, Christine; Cheng, Jonathan C.; Nguyen, Carvell T.; Weisenberger, Daniel J.; Egger, Gerda; Takai, Daiya; Gonzales, Felicidad A.; Jones, Peter A.

    2004-01-01

    Almost 1-2% of the human genome is located within 500 bp of either side of a transcription initiation site, whereas a far larger proportion (≈25%) is potentially transcribable by elongating RNA polymerases. This observation raises the question of how the genome is packaged into chromatin to allow start sites to be recognized by the regulatory machinery at the same time as transcription initiation, but not elongation, is blocked in the 25% of intragenic DNA. We developed a chromatin scanning technique called ChAP, coupling the chromatin immunoprecipitation assay with arbitrarily primed PCR, which allows for the rapid and unbiased comparison of histone modification patterns within the eukaryotic nucleus. Methylated lysine 4 (K4) and acetylated K9/14 of histone H3 were both highly localized to the 5′ regions of transcriptionally active human genes but were greatly decreased downstream of the start sites. Our results suggest that the large transcribed regions of human genes are maintained in a deacetylated conformation in regions read by elongating polymerase. Common models depicting widespread histone acetylation and K4 methylation throughout the transcribed unit do not therefore apply to the majority of human genes. PMID:15123803

  4. Potent and Selective KDM5 Inhibitor Stops Cellular Demethylation of H3K4me3 at Transcription Start Sites and Proliferation of MM1S Myeloma Cells.

    Science.gov (United States)

    Tumber, Anthony; Nuzzi, Andrea; Hookway, Edward S; Hatch, Stephanie B; Velupillai, Srikannathasan; Johansson, Catrine; Kawamura, Akane; Savitsky, Pavel; Yapp, Clarence; Szykowska, Aleksandra; Wu, Na; Bountra, Chas; Strain-Damerell, Claire; Burgess-Brown, Nicola A; Ruda, Gian Filippo; Fedorov, Oleg; Munro, Shonagh; England, Katherine S; Nowak, Radoslaw P; Schofield, Christopher J; La Thangue, Nicholas B; Pawlyn, Charlotte; Davies, Faith; Morgan, Gareth; Athanasou, Nick; Müller, Susanne; Oppermann, Udo; Brennan, Paul E

    2017-03-16

    Methylation of lysine residues on histone tail is a dynamic epigenetic modification that plays a key role in chromatin structure and gene regulation. Members of the KDM5 (also known as JARID1) sub-family are 2-oxoglutarate (2-OG) and Fe(2+)-dependent oxygenases acting as histone 3 lysine 4 trimethyl (H3K4me3) demethylases, regulating proliferation, stem cell self-renewal, and differentiation. Here we present the characterization of KDOAM-25, an inhibitor of KDM5 enzymes. KDOAM-25 shows biochemical half maximal inhibitory concentration values of <100 nM for KDM5A-D in vitro, high selectivity toward other 2-OG oxygenases sub-families, and no off-target activity on a panel of 55 receptors and enzymes. In human cell assay systems, KDOAM-25 has a half maximal effective concentration of ∼50 μM and good selectivity toward other demethylases. KDM5B is overexpressed in multiple myeloma and negatively correlated with the overall survival. Multiple myeloma MM1S cells treated with KDOAM-25 show increased global H3K4 methylation at transcriptional start sites and impaired proliferation. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  5. The histone H3K4-specific demethylase KDM5B binds to its substrate and product through distinct PHD fingers

    Science.gov (United States)

    Klein, Brianna J.; Piao, Lianhua; Xi, Yuanxin; Rincon-Arano, Hector; Rothbart, Scott B.; Peng, Danni; Wen, Hong; Larson, Connie; Zhang, Xi; Zheng, Xia; Cortazar, Michael A.; Peña, Pedro V.; Mangan, Anthony; Bentley, David L.; Strahl, Brian D.; Groudine, Mark; Li, Wei; Shi, Xiaobing; Kutateladze, Tatiana G.

    2014-01-01

    SUMMARY The histone lysine demethylase KDM5B regulates gene transcription and cell differentiation. It contains three PHD fingers, the biological roles of which remain elusive. Here, we show that the first PHD1 finger of KDM5B binds unmodified histone H3, whereas the third PHD3 finger prefers the trimethylated mark, H3K4me3. RNA-seq analysis indicates that KDM5B functions as a transcriptional repressor for a set of genes. Biochemical analysis reveals that KDM5B associates with components of the nucleosome remodeling and deacetylase (NuRD) complex and may cooperate with HDAC1 in gene repression. Compared with the estrogen receptor positive breast cancers, KDM5B is downregulated in the triple-negative breast cancer. Overexpression of KDM5B in the MDA-MB 231 breast cancer cells suppresses cell migration and invasion ability, and the PHD1-H3K4me0 interaction is important for inhibition of migration. These findings highlight tumor-suppressive functions of KDM5B in triple-negative breast cancer cells and suggest a novel multivalent mechanism for KDM5B-mediated transcriptional regulation. PMID:24412361

  6. K4I3 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  7. K4C0 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  8. K4V0 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  9. K4M9 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  10. K4A9 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  11. Matematikkens historie

    DEFF Research Database (Denmark)

    Hansen, Vagn Lundsgaard

    2009-01-01

    Matematikkens historie i syv kapitler: 1. Matematik i støbeskeen; 2. Matematikkens græske arv; 3. Den gyldne tidsalder for hinduer og arabere; 4. Matematik i Kina; 5. Renæssancens matematik; 6. Regning med infinitesimaler ser dagens lys; 7. Matematik i det tyvende århundrede.......Matematikkens historie i syv kapitler: 1. Matematik i støbeskeen; 2. Matematikkens græske arv; 3. Den gyldne tidsalder for hinduer og arabere; 4. Matematik i Kina; 5. Renæssancens matematik; 6. Regning med infinitesimaler ser dagens lys; 7. Matematik i det tyvende århundrede....

  12. Linjefaget historie

    DEFF Research Database (Denmark)

    Rasch-Christensen, Andreas

    Afhandlingen er en undersøgelse af linjefaget historie ved læreruddannelsen. Med fokus på subjektperspektivet peger afhandlingen på en række afgørende udviklingsperspektiver for læreruddannelsen, uddannelsen af historielærere og folkeskolens historieundervisning.......Afhandlingen er en undersøgelse af linjefaget historie ved læreruddannelsen. Med fokus på subjektperspektivet peger afhandlingen på en række afgørende udviklingsperspektiver for læreruddannelsen, uddannelsen af historielærere og folkeskolens historieundervisning....

  13. Matematikkens historie

    DEFF Research Database (Denmark)

    Hansen, Vagn Lundsgaard

    2009-01-01

    Matematikkens historie i syv kapitler: 1. Matematik i støbeskeen; 2. Matematikkens græske arv; 3. Den gyldne tidsalder for hinduer og arabere; 4. Matematik i Kina; 5. Renæssancens matematik; 6. Regning med infinitesimaler ser dagens lys; 7. Matematik i det tyvende århundrede.......Matematikkens historie i syv kapitler: 1. Matematik i støbeskeen; 2. Matematikkens græske arv; 3. Den gyldne tidsalder for hinduer og arabere; 4. Matematik i Kina; 5. Renæssancens matematik; 6. Regning med infinitesimaler ser dagens lys; 7. Matematik i det tyvende århundrede....

  14. Environmental history

    DEFF Research Database (Denmark)

    Pawson, Eric; Christensen, Andreas Aagaard

    2016-01-01

    Environmental history is an interdisciplinary pursuit that has developed as a form of conscience to counter an increasingly powerful, forward-looking liberal theory of the environment. It deals with the relations between environmental ideas and materialities, from the work of the geographers George...... risks”. These are exposed by environmental history’s focus on long-run analysis and its narrative form that identifies the stories that we tell ourselves about nature. How a better understanding of past environmental transformations helps to analyse society and agency, and what this can mean...... for solutions and policies, is the agenda for an engaged environmental history from now on....

  15. Effects of histone acetylation and H3K4 methylation on PRA/PRB in human uterine smooth muscle cells during pregnancy%组蛋白H3、H4乙酰化及H3K4甲基化对人妊娠子宫平滑肌细胞PRA/PRB的影响

    Institute of Scientific and Technical Information of China (English)

    罗慧; 陈诚; 梁志清

    2014-01-01

    目的 通过组蛋白去乙酰化酶抑制剂(trichostatin A,TSA)、H3 K4甲基化酶抑制剂(5’-deoxy-5’-methylthioadenosine,MTA)处理人妊娠子宫平滑肌细胞,干预组蛋白H3、H4乙酰化及H3K4甲基化水平,探讨组蛋白H3、H4乙酰化及H3K4甲基化对人妊娠子宫平滑肌细胞PRA/PRB的影响.方法 分离纯化人妊娠子宫平滑肌细胞(n=16),免疫组化定位孕激素受体(progesterone receptor,PR)及孕激素受体B(progesterone receptor B,PRB)在子宫平滑肌细胞核的表达.分别利用不同浓度TSA、MTA对其进行处理,Real-time PCR检测PR、PRA、PRB mRNA的表达;染色质免疫共沉淀技术(Chromatin immunoprecipitation,ChIP)比较处理前后PRA、PRB启动子区H3、H4乙酰化及H3K4三甲基化水平.结果 TSA可使PRA/PRB明显增高(P<0.05),使PRA启动子区H3、H4乙酰化水平明显上升(P<0.05).MTA可使PRA/PRB明显下降(P<0.05),PRA启动子区H3K4乙酰化水平明显下降(P<0.05).两种药物的干预主要通过对PRA的调控来调节PRA/PRB比值.结论 组蛋白H3、H4乙酰化和H3K4甲基化均可使人妊娠子宫平滑肌细胞PRA/PRB比值发生改变,可能参与“功能性孕激素撤退”机制的调节.

  16. MicroRNA-25通过调控MAP2 K4抑制糖尿病肾病纤维化的研究%Study of Inhibition Fibrosis of MicroRNA-25 on Diabetic Nephropathy by Regulating Expression of MAP2 K4

    Institute of Scientific and Technical Information of China (English)

    王晓莉; 刘洁婷; 张春雷; 王超男; 冯彪; 初彦辉; 张涛

    2015-01-01

    Objective To determine the expression level of microRNA-25 in animal models of diabetic nephropathy and human renal tubular epithelial cells (HK-2) cultured in different conditions, and to explore its regulating effect on the fibrosis in diabetic nephropathy. Methods The expression of microRNA-25 was detected by real-time PCR. The downstream target protein of microRNA-25 was verified by bioinformatic prediction, transient transfection of cells and Western blotting. Results MicroRNA-25 was down-regulated in animal models of diabetic nephropathy and HK-2 cells which were cultured in high-glucose medium (P<0. 01). MAP2K4 might be the downstream target protein of microRNA-25. Overexpression of microRNA-25 reduced the protein expression of MAP2K4 and α-SMA (P<0. 01). Conclusion MicroRNA-25 inhibits the fibrosis in diabetic nephropathy by regulating the expression of MAP2K4.%目的:检测microRNA-25在糖尿病肾病动物模型及不同条件培养下的人肾小管上皮细胞( HK-2)中的表达,探讨其对糖尿病肾病纤维化的调节作用。方法实时荧光定量聚合酶链反应检测microRNA-25的表达。生物信息学预测、细胞瞬时转染和Western blot验证microRNA-25的下游靶蛋白。结果 microRNA-25在糖尿病肾病动物模型及高糖培养下的HK-2中表达降低(P<0.01)。 MAP2K4可能是microRNA-25的下游靶蛋白。过表达microRNA-25可以从蛋白水平上抑制MAP2K4、α-SMA的表达(P<0.01)。结论 microRNA-25可以通过调控MAP2K4从而抑制糖尿病肾病纤维化的进展。

  17. Cultural history as polyphonic history

    Directory of Open Access Journals (Sweden)

    Burke, Peter

    2010-06-01

    Full Text Available This texts offers a reflection on the origins and actual development of the field of cultural history through a comparison with the term that has served as title for this seminar: “polyphonic history”. The author provides an overview of the themes that have structured the seminar (the history of representations, the history of the body and the cultural history of science with the aim of making explicit and clarifying this plurality of voices in the field of history as well as its pervasiveness in other research areas.

    En este texto se ofrece una reflexión sobre el origen y actual desarrollo del campo de la historia cultural a través de una comparación con el término que ha dado título a este seminario: “historia polifónica”. El autor propone un recorrido por las áreas temáticas que han conformado la estructura del seminario (la historia de las representaciones, la historia del cuerpo y la historia cultural de la ciencia con el objeto de explicitar y explicar esta pluralidad de voces en el campo de la historia, así como su repercusión en otras áreas del conocimiento.

  18. How do lay people assess the quality of physicians' communicative responses to patients' emotional cues and concerns? An international multicentre study based on videotaped medical consultations.

    NARCIS (Netherlands)

    Mazzi, M.A.; Bensing, J.; Rimondini, M.; Fletcher, I.; Vliet, L. van; Zimmermann, C.; Deveugele, M.

    2013-01-01

    Objective: To establish which kind of physician communicative responses to patient cues and concerns are appreciated by lay people. Methods: A balanced sample (259 people) was recruited in public places to participate in a full day observation of four videotaped standardized medical consultations. I

  19. The efficacy of videotape feedback for enhancing the effects of exposure-based treatment for social anxiety disorder: A controlled investigation

    NARCIS (Netherlands)

    Smits, J.A.J.; Powers, M.B.; Buxkamper, R.; Telch, M.J.

    2006-01-01

    Correcting patients' faulty beliefs concerning social evaluative threats is the hallmark of cognitive-behavioral treatment of social anxiety disorder. The current study examined the efficacy of two videotape feedback procedures as adjuncts to exposure-based treatment. Participants suffering from soc

  20. Examining the content of weight, nutrition and physical activity advices provided by Dutch practice nurses in primary care: analysis of videotaped consultations.

    NARCIS (Netherlands)

    Dillen, S.M.E. van; Noordman, J.; Dulmen, S. van; Hiddink, G.J.

    2014-01-01

    Background/Objective: To examine the content of Dutch practice nurses’ (PNs’) advices about weight, nutrition and physical activity to overweight and obese patients. Subjects/Methods: A 100 videotaped real-life PN consultations (The Netherlands, 2010/2011) with overweight or obese patients were sele

  1. Examining the content of weight, nutrition and physical activity advices provided by Dutch practice nurses in primary care: analysis of videotaped consultations

    NARCIS (Netherlands)

    Dillen, S.M. van; Noordman, J.; Dulmen, S. van; Hiddink, G.J.

    2014-01-01

    BACKGROUND/OBJECTIVE: To examine the content of Dutch practice nurses' (PNs') advices about weight, nutrition and physical activity to overweight and obese patients. SUBJECTS/METHODS: A 100 videotaped real-life PN consultations (The Netherlands, 2010/2011) with overweight or obese patients were

  2. Examining the content of weight, nutrition and physical activity advices provided by Dutch practice nurses in primary care: analysis of videotaped consultations.

    NARCIS (Netherlands)

    Dillen, S.M.E. van; Noordman, J.; Dulmen, S. van; Hiddink, G.J.

    2014-01-01

    Background/Objective: To examine the content of Dutch practice nurses’ (PNs’) advices about weight, nutrition and physical activity to overweight and obese patients. Subjects/Methods: A 100 videotaped real-life PN consultations (The Netherlands, 2010/2011) with overweight or obese patients were

  3. The Writing on the Wall: A Fox Family Channel Afternoon Special for Cable in the Classroom. [Videotape with] Guide for Educators.

    Science.gov (United States)

    KIDSNET, Washington, DC.

    "The Writing on the Wall," the television program featured in this videotape and teaching guide, focuses on three suburban teenagers who deface with anti-Semitic graffiti, the property of a rabbi, a holocaust survivor, and the Jewish community center and synagogue. The teenagers, tried and convicted, are spared a harsh sentence when the…

  4. Why History?

    Science.gov (United States)

    Duffy, Robert E.

    1988-01-01

    Examines the way in which studying history contributes to intellectual development. Identifies five mental attributes it enhances: perspective--gained from placing people, events, institutions against larger background; encounter--confronting great ideas, personalities, etc.; relativism in a pluralistic world--developed from immersion in other…

  5. Bulletproof History.

    Science.gov (United States)

    Roy, R. H.

    1994-01-01

    Asserts that the writers and producers of the television documentary, "The Valour and the Horror," provided a false impression of an event to fit preconceived and erroneous interpretations of history. Points out specific examples of inaccurate historical presentations and provides contradictory historical interpretations. (CFR)

  6. Business History

    DEFF Research Database (Denmark)

    Hansen, Per H.

    2012-01-01

    This article argues that a cultural and narrative perspective can enrich the business history field, encourage new and different questions and answers, and provide new ways of thinking about methods and empirical material. It discusses what culture is and how it relates to narratives. Taking...

  7. Potted history

    NARCIS (Netherlands)

    Van Dijk, T.

    2010-01-01

    The Jordan Valley was once populated by a people, now almost forgotten by historians, with whom the pharaoh of Egypt sought favour. That is the conclusion reached by Niels Groot, the first researcher to take a PhD at the Delft-Leiden Centre for Archaeology, Art History and Science.

  8. K4Sn3Se8的固相合成、晶体结构和反射光谱研究%Solid state synthesis,crystal structure and diffuse reflectance spectra of K4Sn3Se8

    Institute of Scientific and Technical Information of China (English)

    王志霞; 郭洪猷

    2003-01-01

    采用反应性熔盐法,通过中温固相反应在500 ℃下合成晶体K4Sn3Se8.该晶体属正交晶系,空间群Ccca,晶胞参数:a=0.821 15(6) nm,b=2.779 0(2) nm,c=0.818 65(6) nm,V=1.868 2(2) nm3,Z=4.K4Sn3Se8由[Sn3Se8]4-三聚体负离子和K+正离子组成.漫反射光谱研究表明该材料能隙(Eg)为2.6 eV,属于半导体,对太阳能具有选择吸收的特性.

  9. H3K4 dimethylation in hepatocellular carcinoma is rare compared with other hepatobiliary and gastrointestinal carcinomas and correlates with expression of the methylase Ash2 and the demethylase LSD1.

    Science.gov (United States)

    Magerl, Christian; Ellinger, Jörg; Braunschweig, Till; Kremmer, Elisabeth; Koch, Lin Kristin; Höller, Tobias; Büttner, Reinhard; Lüscher, Bernhard; Gütgemann, Ines

    2010-02-01

    Methylation of core histones regulates chromatin structure and gene expression. Recent studies have demonstrated that these methylation patterns have prognostic value for some tumors. Therefore, we investigated dimethylation of histone H3 at lysine 4 (H3K4diMe) and H3K4 methylating (Ash2 complex) and demethylating enzymes (LSD1) in carcinomas of the hepatic and gastrointestinal tract. High levels of H3K4diMe were rarely observed in 15.7% of hepatocellular carcinoma (8/51) unlike other carcinomas including, in ascending order, cholangiocellular carcinoma/adenocarcinoma of the extrahepatic biliary tract, gastric carcinoma, pancreatic ductal adenocarcinoma, and neuroendocrine carcinoma (P carcinomas (38/45) and correlated directly with H3K4diMe modification (correlation coefficient r = 0.53) and LSD1 expression (r = 0.35). In contrast to other carcinomas, 65.9% (29/44) of hepatocellular carcinomas analyzed showed no LSD1 expression (P carcinomas without LSD1 expression appeared to be frequently Ash2 and H3K4diMe weak or negative (P = .004). In summary, high H3K4diMe expression is rare in hepatocellular carcinoma compared with other carcinomas (negative predictive value 92.3%), which may aid in the differential diagnosis. Lack of H3K4diMe is possibly due to complex epigenetic regulation involving Ash2 and LSD1.

  10. Final Report on the Key Comparison CCM.P-K4.2012 in Absolute Pressure from 1 Pa to 10 kPa.

    Science.gov (United States)

    Ricker, Jacob; Hendricks, Jay; Bock, Thomas; Dominik, Pražák; Kobata, Tokihiko; Torres, Jorge; Sadkovskaya, Irina

    2017-01-01

    The report summarizes the Consultative Committee for Mass (CCM) key comparison CCM.P-K4.2012 for absolute pressure spanning the range of 1 Pa to 10 000 Pa. The comparison was carried out at six National Metrology Institutes (NMIs), including National Institute of Standards and Technology (NIST), Physikalisch-Technische Bundesanstalt (PTB), Czech Metrology Institute (CMI), National Metrology Institute of Japan (NMIJ), Centro Nacional de Metrología (CENAM), and DI Mendeleyev Institute for Metrology (VNIIM). The comparison was made via a calibrated transfer standard measured at each of the NMIs facilities using their laboratory standard during the period May 2012 to September 2013. The transfer package constructed for this comparison preformed as designed and provided a stable artifact to compare laboratory standards. Overall the participants were found to be statistically equivalent to the key comparison reference value.

  11. Chromosomal distribution of H3K4me2, H3K9me2 and 5-methylcytosine: variations associated with polyploidy and hybridization in Brachiaria (Poaceae).

    Science.gov (United States)

    de Paula, Cristina Maria Pinto; Souza Sobrinho, Fausto; Techio, Vânia Helena

    2016-06-01

    Assessment of chromosomal distribution of modified histones and 5-methylcytosine shown that there are diversification of chromosomal types among species of Brachiaria and its interspecific hybrids. Histone post-translational modifications and DNA methylation are epigenetic processes that are involved in structural and functional organization of the genome. This study compared the chromosomal distribution of modified histones and 5-methylcytosine (5-mCyt) in species and interspecific hybrids of Brachiaria with different ploidy levels and reproduction modes. The relation between H3K9me2 and 5-mCyt was observed in the nucleolus organizer region, centromeric central domain and pericentromeric region. H3K4me2 was detected in euchromatic domains, mainly in the terminal chromosomal regions. Comparison of chromosomal distribution among species and hybrids showed greater variation of chromosomal types for the H3K9me2 in B. decumbens (tetraploid and apomictic species) and the 963 hybrid, while, for the H3K4me2, the variation was higher in B. brizantha and B. decumbens (tetraploid and apomictic species) and 963 hybrid. The chromosome distribution of 5-mCyt was similar between B. brizantha and B. decumbens, which differ from the distribution observed in B. ruziziensis (diploid and sexual species). Significant alterations in DNA methylation were observed in the artificially tetraploidized B. ruziziensis and in the interspecific hybrids, possibly as result of hybridization and polyploidization processes. The monitoring of histone modifications and DNA methylation allowed categorizing nuclear and chromosomal distribution of these epigenetic marks, thus contributing to the knowledge of composition and structure of the genome/epigenome of Brachiaria species and hybrids. These data can be useful for speciation and genome evolution studies in genus Brachiaria, and represent important markers to explore relationships between genomes.

  12. Business History as Cultural History

    DEFF Research Database (Denmark)

    Lunde Jørgensen, Ida

    The paper engages with the larger question of how cultural heritage becomes taken for granted and offers a complimentary view to the anthropological ʻCopenhagen School’ of business history, one that draws attention to the way corporate wealth directly and indirectly influences the culture available...

  13. Sommerferiens historie

    DEFF Research Database (Denmark)

    Lützen, Karin

    2011-01-01

    favourite ways for com- mon people to spend their holidays, and with the introduction of holiday pay in the 1930s almost everybody could take a couple of weeks off work in the sum- mer. With the introduction of charter tourism many people went off to Southern Europe to spend their holidays on the same...... pattern. Finally, the history of the special holiday camps is told, which were established by American Jews because they were excluded from many hotels....

  14. Effect of sleep deprivation on H3K9 and H3K4 methylation in the different brain region of rats%REM期睡眠剥夺对大鼠不同脑区组蛋白H3K9和H3K4甲基化水平的影响

    Institute of Scientific and Technical Information of China (English)

    马安东; 马妮; 史明; 田丰仓; 吴中亮

    2016-01-01

    目的:研究REM期睡眠剥夺对成年大鼠不同脑区包括海马、腹内侧前额叶皮质、下丘脑和中缝核群的组蛋白H3K9和H3K4三甲基化水平的影响.方法:将成年SD大鼠随机分为正常对照组(con)、睡眠剥夺1d组(SD1 d)、睡眠剥夺3d组(SD3 d)、睡眠剥夺6d组(SD6 d).采用改良式多平台水环境法建立REM期睡眠剥夺模型,分别于睡眠剥夺后1、3、6d断头取脑,用免疫荧光染色与Western Blot检测大鼠海马、腹内侧前额叶皮质、下丘脑和中缝核群H3K9和H3K4三甲基化水平的变化,最终结果进行统计学分析.结果:Western Blot结果显示:(1)海马、腹内侧前额叶皮质三个SD组H3K9三甲基化水平均低于control组(P<0.05),H3K4三甲基化水平均高于control组(P<0.05);(2)下丘脑三个SD组H3K9三甲基化水平均低于control组(P<0.01),但SD1 d组H3K4三甲基化水平高于control组(P<0.05),SD3 d、SD6 d组H3K4三甲基化水平低于control组(P<0.05);(3)中缝核群三个SD组H3K9和H3K4三甲基化水平均高于control组(P<0.05).对海马与下丘脑免疫荧光验证H3K9三甲基化结果与Western Blot结果趋势一致,且说明细胞无减少.结论:睡眠剥夺可能与海马、腹内侧前额叶皮质、下丘脑和中缝核群的组蛋白H3K9和H3K4三甲基化水平密切相关.

  15. Uncovering History for Future History Teachers

    Science.gov (United States)

    Fischer, Fritz

    2010-01-01

    The art of history teaching is at a crossroads. Recent scholarship focuses on the need to change the teaching of history so students can better learn history, and insists that history teachers must move beyond traditional structures and methods of teaching in order to improve their students' abilities to think with history. This article presents…

  16. Ildens historier

    DEFF Research Database (Denmark)

    Lassen, Henrik Roesgaard

    In December 2012 a manuscript entitled "Tællelyset" ['The Tallow Candle'] was discovered in an archive. The story was subsequently presented to the world as Hans Christian Andersen's first fairy tale and rather bombastically celebrated as such. In this book it is demonstrated that the text cannot...... from a point-by-point tracing of 'the origins and history' of Hans Christian Andersen's famous fairy tales. Where did the come from? How did they become the iconic texts that we know today? On this background it becomes quite clear that "Tællelyset" is a modern pastiche and not a genuine Hans Christian...... Andersen fairy tale....

  17. Dot1-dependent histone H3K79 methylation promotes the formation of meiotic double-strand breaks in the absence of histone H3K4 methylation in budding yeast.

    Directory of Open Access Journals (Sweden)

    Mohammad Bani Ismail

    Full Text Available Epigenetic marks such as histone modifications play roles in various chromosome dynamics in mitosis and meiosis. Methylation of histones H3 at positions K4 and K79 is involved in the initiation of recombination and the recombination checkpoint, respectively, during meiosis in the budding yeast. Set1 promotes H3K4 methylation while Dot1 promotes H3K79 methylation. In this study, we carried out detailed analyses of meiosis in mutants of the SET1 and DOT1 genes as well as methylation-defective mutants of histone H3. We confirmed the role of Set1-dependent H3K4 methylation in the formation of double-strand breaks (DSBs in meiosis for the initiation of meiotic recombination, and we showed the involvement of Dot1 (H3K79 methylation in DSB formation in the absence of Set1-dependent H3K4 methylation. In addition, we showed that the histone H3K4 methylation-defective mutants are defective in SC elongation, although they seem to have moderate reduction of DSBs. This suggests that high levels of DSBs mediated by histone H3K4 methylation promote SC elongation.

  18. Final report on the key comparison CCM.P-K4.2012 in absolute pressure from 1 Pa to 10 kPa

    Science.gov (United States)

    Ricker, Jacob; Hendricks, Jay; Bock, Thomas; Dominik, Pražák; Kobata, Tokihiko; Torres, Jorge; Sadkovskaya, Irina

    2017-01-01

    The report summarizes the Consultative Committee for Mass (CCM) key comparison CCM.P-K4.2012 for absolute pressure spanning the range of 1 Pa to 10 000 Pa. The comparison was carried out at six National Metrology Institutes (NMIs), including National Institute of Standards and Technology (NIST), Physikalisch-Technische Bundesanstalt (PTB), Czech Metrology Institute (CMI), National Metrology Institute of Japan (NMIJ), Centro Nacional de Metrología (CENAM), and DI Mendeleyev Institute for Metrology (VNIIM). The comparison was made via a calibrated transfer standard measured at each of the NMIs facilities using their laboratory standard during the period May 2012 to September 2013. The transfer package constructed for this comparison preformed as designed and provided a stable artifact to compare laboratory standards. Overall the participants were found to be statistically equivalent to the key comparison reference value. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  19. Magnetic transition in K4Cu4OCl10: A model system of three-dimensional spin-(1)/(2) tetrahedra

    Science.gov (United States)

    Fujihala, Masayoshi; Zheng, Xu-Guang; Morodomi, Hiroki; Kawae, Tatsuya; Watanabe, Isao

    2013-04-01

    Isolated spin tetrahedral systems with weak intertetrahedral couplings, as have been reported for Cu2Te2O5X2 (X = Cl, Br) and the related compound Cu4Te5O12Cl4, have received much attention recently because they represent an interesting class of magnets that consist of weakly coupled magnetic clusters and, in particular, they can directly demonstrate the interplay of intertetrahedral couplings with built-in tetrahedral frustration. However, there is much debate about the structural low dimensionality of the Cu-Te-O-Cl(Br) compounds and its effect on the magnetism of the material. Here, we present a model spin tetrahedral system K4Cu4OCl10, with almost isotropic magnetic coupling within the tetrahedron and three-dimensional connection of the tetrahedra. The system enters a spin-singlet state with a susceptibility maximum at Tmax = 11 K, and then enters an antiferromagnetic order at TN = 4.4 K. The ratio TN/Tmax = 0.40 is close to the TN/Tmax = 0.38 for Cu2Te2O5Br2, which is viewed as an indicator of closeness to quantum criticality. Evidence in muon-spin rotation or relaxation suggests an incommensurate ordering. This work shows that the previously revealed ground state in anisotropically structured Cu2Te2O5X2 compounds also exists in an isotropic spin tetrahedral system.

  20. South Experimental Oculina Research Reserve, Clelia Dive 610 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  1. Central Experimental Oculina Research Reserve, Clelia Dive 612 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  2. Public History

    Directory of Open Access Journals (Sweden)

    Marta Gouveia de Oliveira Rovai

    2017-04-01

    Full Text Available Este artigo tem como proposta apresentar o conceito e as práticas de História Pública como um novo posicionamento da ciência histórica em diálogo com profissionais da comunicação, no sentido de produzir e divulgar as experiências humanas. Para isso, discute-se a origem do conceito de História Pública e as diferentes formas de educação histórica que a utilização das novas tecnologias podem proporcionar (dentre elas a internet. Nesse sentido, convida-se o leitor para a reflexão sobre as possibilidades de publicização e de democratização do conhecimento histórico e da cultura, ampliando-se a oportunidade de produção, de divulgação e de acesso do público a diferentes formas experiências no tempo. O artigo também intenciona chamar atenção dos profissionais que lidam com a História e com a Comunicação para os perigos de produções exclusivamente submetidas ao mercado que transformam a popularização da História no reforço de estigmas culturais.   PALAVRAS-CHAVE: História Pública; Educação histórica e Comunicação; democratização e estigmatização.     ABSTRACT This article aims to present the concept and practices of Public History as a new positioning of historical science in dialogue with communication professionals, in the sense of producing and disseminating human experiences. For this, the origin of the concept of Public History and the different forms of historical education that the use of the new technologies can provide (among them the Internet is discussed. In this sense, the reader is invited to reflect on the possibilities of publicizing and democratizing historical knowledge and culture, expanding the opportunity for production, dissemination and public access to different forms of experience in time. The article also intends to draw attention from professionals dealing with History and Communication to the dangers of exclusively commercialized productions that transform the popularization

  3. The Future of History and History Teaching.

    Science.gov (United States)

    Commager, Henry Steele

    1983-01-01

    Technical history, a quantitative record of history strengthened by new techniques in mathematics, computer science, and other fields has advantages over former approaches to history--history as philosophy and historical theology. For example, it makes available more source materials. However, it has drawbacks, e.g., it directs research to highly…

  4. The Future of History and History Teaching.

    Science.gov (United States)

    Commager, Henry Steele

    1983-01-01

    Technical history, a quantitative record of history strengthened by new techniques in mathematics, computer science, and other fields has advantages over former approaches to history--history as philosophy and historical theology. For example, it makes available more source materials. However, it has drawbacks, e.g., it directs research to highly…

  5. Final report on the EURAMET.M.FF-K4.2.2014 volume comparison at 100 μL—calibration of micropipettes

    Science.gov (United States)

    Batista, Elsa; Matus, Michael; Metaxiotou, Zoe; Tudor, Maria; Lenard, Elzbieta; Buker, Oliver; Wennergren, Per; Piluri, Erinda; Miteva, Mariana; Vicarova, Martina; Vospĕlová, Alena; Turnsek, Urska; Micic, Ljiljana; Grue, Lise-Lote; Mihailovic, Mirjana; Sarevska, Anastazija

    2017-01-01

    During the EURAMET TC-F meeting of 2014 and following the finalization of CCM.FF-K4.2.2011 comparison, it was agreed to start a Regional Key Comparison (KC) on volume measurements using two 100 μL micropipettes (piston pipettes) allowing the participating laboratories to assess the agreement of their results and uncertainties. Two 100 μL micropipettes were tested by 15 participants. One participant was not a member or associate member of the BIPM and was be removed from this report. The comparison started in July 2015 and ended in March 2016. The Volume and Flow Laboratory of the Portuguese Institute for Quality (IPQ) was the pilot laboratory and performed the initial and final measurements of the micropipettes. The micropipettes showed a stable volume during the whole comparison, which was confirmed by the results from the pilot laboratory. The original results of all participant NMIs were corrected to the standard atmospheric pressure in order to compare results under the same calibration conditions, and the contribution of the 'process-related handling contribution' was added to the uncertainty budget of each participant. In general the declared CMCs are in accordance with the KCDB. For the micropipette 354828Z, two laboratories had inconsistent results. For micropipette 354853Z, three laboratories had inconsistent results. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  6. RSV-Induced H3K4 Demethylase KDM5B Leads to Regulation of Dendritic Cell-Derived Innate Cytokines and Exacerbates Pathogenesis In Vivo.

    Directory of Open Access Journals (Sweden)

    Catherine Ptaschinski

    2015-06-01

    Full Text Available Respiratory syncytial virus (RSV infection can result in severe disease partially due to its ability to interfere with the initiation of Th1 responses targeting the production of type I interferons (IFN and promoting a Th2 immune environment. Epigenetic modulation of gene transcription has been shown to be important in regulating inflammatory pathways. RSV-infected bone marrow-derived DCs (BMDCs upregulated expression of Kdm5b/Jarid1b H3K4 demethylase. Kdm5b-specific siRNA inhibition in BMDC led to a 10-fold increase in IFN-β as well as increases in IL-6 and TNF-α compared to control-transfected cells. The generation of Kdm5bfl/fl-CD11c-Cre+ mice recapitulated the latter results during in vitro DC activation showing innate cytokine modulation. In vivo, infection of Kdm5bfl/fl-CD11c-Cre+ mice with RSV resulted in higher production of IFN-γ and reduced IL-4 and IL-5 compared to littermate controls, with significantly decreased inflammation, IL-13, and mucus production in the lungs. Sensitization with RSV-infected DCs into the airways of naïve mice led to an exacerbated response when mice were challenged with live RSV infection. When Kdm5b was blocked in DCs with siRNA or DCs from Kdm5bfl/fl-CD11c-CRE mice were used, the exacerbated response was abrogated. Importantly, human monocyte-derived DCs treated with a chemical inhibitor for KDM5B resulted in increased innate cytokine levels as well as elicited decreased Th2 cytokines when co-cultured with RSV reactivated CD4+ T cells. These results suggest that KDM5B acts to repress type I IFN and other innate cytokines to promote an altered immune response following RSV infection that contributes to development of chronic disease.

  7. Role of RbBP5 and H3K4me3 in the vicinity of Snail transcription start site during epithelial-mesenchymal transition in prostate cancer cell.

    Science.gov (United States)

    Li, Dong; Sun, Hui; Sun, Wen-Jing; Bao, Hong-Bo; Si, Shu-Han; Fan, Jia-Lin; Lin, Ping; Cui, Rong-Jun; Pan, Yu-Jia; Wen, Si-Min; Zheng, Xiu-Lan; Yu, Xiao-Guang

    2016-10-04

    EMT (epithelial-mesenchymal transition) occurs in a wide range of tumor types, and has been shown to be crucial for metastasis. Epigenetic modifications of histones contribute to chromatin structure and result in the alterations in gene expression. Tri-methylation of histone H3 lysine 4 (H3K4me3) is associated with the promoters of actively transcribed genes and can serve as a transcriptional on/off switch. RbBP5 is a component of the COMPASS/ -like complex, which catalyzes H3K4me3 formation. In this study, we found that in the process of TGF-Beta1 induced EMT in the prostate cancer cell line DU145, H3K4me3 enrichment and RbBP5 binding increased in the vicinity of Snail (SNAI1) transcription start site. Knocking-down of RbBP5 notably decreased Snail expression and EMT. Recruitment of RbBP5 and formation of H3K4me3 at Snail TSS during EMT depend on binding of SMAD2/3 and CBP at Snail TSS. This study links the SMAD2/3 signal with Snail transcription via a histone modification - H3K4me3. Furthermore, our research also demonstrates that RbBP5 and even WRAD may be a promising therapeutic candidates in treating prostate cancer metastasis, and that DU145 cells maintain their incomplete mesenchymal state in an auto/ paracrine manner.

  8. Shifts in doctor-patient communication between 1986 and 2002: a study of videotaped General Practice consultations with hypertension patients

    Directory of Open Access Journals (Sweden)

    van den Brink-Muinen Atie

    2006-10-01

    Full Text Available Abstract Background Departing from the hypotheses that over the past decades patients have become more active participants and physicians have become more task-oriented, this study tries to identify shifts in GP and patient communication patterns between 1986 and 2002. Methods A repeated cross-sectional observation study was carried out in 1986 and 2002, using the same methodology. From two existing datasets of videotaped routine General Practice consultations, a selection was made of consultations with hypertension patients (102 in 1986; 108 in 2002. GP and patient communication was coded with RIAS (Roter Interaction Analysis System. The data were analysed, using multilevel techniques. Results No gender or age differences were found between the patient groups in either study period. Contrary to expectations, patients were less active in recent consultations, talking less, asking fewer questions and showing less concerns or worries. GPs provided more medical information, but expressed also less often their concern about the patients' medical conditions. In addition, they were less involved in process-oriented behaviour and partnership building. Overall, these results suggest that consultations in 2002 were more task-oriented and businesslike than sixteen years earlier. Conclusion The existence of a more equal relationship in General Practice, with patients as active and critical consumers, is not reflected in this sample of hypertension patients. The most important shift that could be observed over the years was a shift towards a more businesslike, task-oriented GP communication pattern, reflecting the recent emphasis on evidence-based medicine and protocolized care. The entrance of the computer in the consultation room could play a role. Some concerns may be raised about the effectiveness of modern medicine in helping patients to voice their worries.

  9. Shifts in doctor-patient communication between 1986 and 2002: a study of videotaped General Practice consultations with hypertension patients

    Science.gov (United States)

    Bensing, Jozien M; Tromp, Fred; van Dulmen, Sandra; van den Brink-Muinen, Atie; Verheul, William; Schellevis, François G

    2006-01-01

    Background Departing from the hypotheses that over the past decades patients have become more active participants and physicians have become more task-oriented, this study tries to identify shifts in GP and patient communication patterns between 1986 and 2002. Methods A repeated cross-sectional observation study was carried out in 1986 and 2002, using the same methodology. From two existing datasets of videotaped routine General Practice consultations, a selection was made of consultations with hypertension patients (102 in 1986; 108 in 2002). GP and patient communication was coded with RIAS (Roter Interaction Analysis System). The data were analysed, using multilevel techniques. Results No gender or age differences were found between the patient groups in either study period. Contrary to expectations, patients were less active in recent consultations, talking less, asking fewer questions and showing less concerns or worries. GPs provided more medical information, but expressed also less often their concern about the patients' medical conditions. In addition, they were less involved in process-oriented behaviour and partnership building. Overall, these results suggest that consultations in 2002 were more task-oriented and businesslike than sixteen years earlier. Conclusion The existence of a more equal relationship in General Practice, with patients as active and critical consumers, is not reflected in this sample of hypertension patients. The most important shift that could be observed over the years was a shift towards a more businesslike, task-oriented GP communication pattern, reflecting the recent emphasis on evidence-based medicine and protocolized care. The entrance of the computer in the consultation room could play a role. Some concerns may be raised about the effectiveness of modern medicine in helping patients to voice their worries. PMID:17064407

  10. Comparative effectiveness of videotape and handout mode of instructions for teaching exercises: skill retention in normal children.

    Science.gov (United States)

    Gupta, Garima; Sehgal, Stuti

    2012-01-30

    Teaching of motor skills is fundamental to physical therapy practice. In order to optimize the benefits of these teaching and training efforts, various forms of patient education material are developed and handed out to patients. One very important fact has been overlooked. While comparative effectiveness of various modes of instruction has been studied in adults, attention has not been paid to the fact that learning capabilities of children are different from that of adults. The intent of the present study is to compare the effectiveness of video and handout mode of instructions specifically on children. A total of 115 normal elementary-age children aged 10 to 12 years of age were studied. The children were randomized into two groups: A) the video group, and B) the handout group. The video group viewed the video for physical therapy exercises while the handout group was provided with paper handouts especially designed according to the readability of their age group. Statistical analysis using the student's't' test showed that subjects of both the video and handout groups exhibited equal overall performance accuracy. There was no significant difference between the groups both in acquisition and retention accuracy tests. The findings of the present study suggest that if the readability and instructional principles applicable to different target age groups are strictly adhered to, then both video as well as handout modes of instructions result in similar feedback and memory recall in ten to twelve year-old children. Principles of readability related to the patient age are of utmost importance when designing the patient education material. These findings suggest that the less expensive handouts can be an effective instructional aid for teaching exercises to children with various neuromuscular, rheumatic, and orthopedics conditions and the most costly videotape techniques are not necessarily better.

  11. Comparative effectiveness of videotape and handout mode of instructions for teaching exercises: skill retention in normal children

    Directory of Open Access Journals (Sweden)

    Gupta Garima

    2012-01-01

    Full Text Available Abstract Background Teaching of motor skills is fundamental to physical therapy practice. In order to optimize the benefits of these teaching and training efforts, various forms of patient education material are developed and handed out to patients. One very important fact has been overlooked. While comparative effectiveness of various modes of instruction has been studied in adults, attention has not been paid to the fact that learning capabilities of children are different from that of adults. The intent of the present study is to compare the effectiveness of video and handout mode of instructions specifically on children. Methods A total of 115 normal elementary-age children aged 10 to 12 years of age were studied. The children were randomized into two groups: A the video group, and B the handout group. The video group viewed the video for physical therapy exercises while the handout group was provided with paper handouts especially designed according to the readability of their age group. Results Statistical analysis using the student's't' test showed that subjects of both the video and handout groups exhibited equal overall performance accuracy. There was no significant difference between the groups both in acquisition and retention accuracy tests. Conclusion The findings of the present study suggest that if the readability and instructional principles applicable to different target age groups are strictly adhered to, then both video as well as handout modes of instructions result in similar feedback and memory recall in ten to twelve year-old children. Principles of readability related to the patient age are of utmost importance when designing the patient education material. These findings suggest that the less expensive handouts can be an effective instructional aid for teaching exercises to children with various neuromuscular, rheumatic, and orthopedics conditions and the most costly videotape techniques are not necessarily better.

  12. Epigenetics and sex differences in the brain: A genome-wide comparison of histone-3 lysine-4 trimethylation (H3K4me3) in male and female mice.

    Science.gov (United States)

    Shen, Erica Y; Ahern, Todd H; Cheung, Iris; Straubhaar, Juerg; Dincer, Aslihan; Houston, Isaac; de Vries, Geert J; Akbarian, Schahram; Forger, Nancy G

    2015-06-01

    Many neurological and psychiatric disorders exhibit gender disparities, and sex differences in the brain likely explain some of these effects. Recent work in rodents points to a role for epigenetics in the development or maintenance of neural sex differences, although genome-wide studies have so far been lacking. Here we review the existing literature on epigenetics and brain sexual differentiation and present preliminary analyses on the genome-wide distribution of histone-3 lysine-4 trimethylation in a sexually dimorphic brain region in male and female mice. H3K4me3 is a histone mark primarily organized as 'peaks' surrounding the transcription start site of active genes. We microdissected the bed nucleus of the stria terminalis and preoptic area (BNST/POA) in adult male and female mice and used ChIP-Seq to compare the distribution of H3K4me3 throughout the genome. We found 248 genes and loci with a significant sex difference in H3K4me3. Of these, the majority (71%) had larger H3K4me3 peaks in females. Comparisons with existing databases indicate that genes and loci with increased H3K4me3 in females are associated with synaptic function and with expression atlases from related brain areas. Based on RT-PCR, only a minority of genes with a sex difference in H3K4me3 has detectable sex differences in expression at baseline conditions. Together with previous findings, our data suggest that there may be sex biases in the use of epigenetic marks. Such biases could underlie sex differences in vulnerabilities to drugs or diseases that disrupt specific epigenetic processes. Copyright © 2014 Elsevier Inc. All rights reserved.

  13. k482ar.m77t - MGD77 data file for Geophysical data from field activity K-4-82-AR in Arctic from 09/13/1982 to 10/10/1982

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Single-beam bathymetry data along with DGPS navigation data was collected as part of field activity K-4-82-AR in Arctic from 09/13/1982 to 10/10/1982,...

  14. Popular history magazines and history education

    Directory of Open Access Journals (Sweden)

    Robert Thorp

    2016-05-01

    Full Text Available This paper argues that popular history magazines may be a welcome complement to other forms of historical media in history teaching. By outlining a theoretical framework that captures uses of history, the paper analyses popular history magazine articles from five European countries all dealing with the outbreak of World War I. The study finds that while the studied articles provide a rather heterogeneous view of the causes of the Great War, they can be used to discuss and analyse the importance of perspective in history, thus offering an opportunity to further a more disciplinary historical understanding.

  15. Measurement of the Energy Expenditures in Daily Physical Activities with K4b2%应用K4b2心肺功能测定仪对日常体力活动能量消耗的测定

    Institute of Scientific and Technical Information of China (English)

    刘健敏; 李颜; 杨晓光

    2010-01-01

    应用K4b2心肺功能测定仪对日常体力活动的能量消耗进行现场测定,以期为我国体力活动指南的制定提供基础资料.选取23名北医青年学生作为受试者,其中男11名,女12名,应用K4b2心肺功能测定仪对受试者在静息、慢走(3 km/h)、快走(6 km/h)、慢跑(8 km/h)、骑自行车(12 km/h)状态下的能量消耗进行测定.各项体力活动的能量消耗及代谢当量由大到小排列,依次为幔跑((6.26±1.22)kcal·kg-1~·h-1,6.0 MET)、骑自行车((4.30±0.67)kcal·kg-1·h-1,4.2 MET)、快走((3.75±0.73)kcal·ks-1·h-1,3.6 MET)、慢走((1.24±0.26)kcal·kg-1·h-1,1.2 MET)、静息((1.08±0.24)kcal·kg-1·h-1,1.0 MET),按照体力活动强度分级,属于中低强度体力活动.慢走、快走、慢跑、骑自行车等日常体力活动适于在中国人群中推广.

  16. Does burnout among doctors affect their involvement in patients' mental health problems? A study of videotaped consultations

    Directory of Open Access Journals (Sweden)

    de Bakker Dinny H

    2009-08-01

    Full Text Available Abstract Background General practitioners' (GPs' feelings of burnout or dissatisfaction may affect their patient care negatively, but it is unknown if these negative feelings also affect their mental health care. GPs' available time, together with specific communication tools, are important conditions for providing mental health care. We investigated if GPs who feel burnt out or dissatisfied with the time available for their patients, are less inclined to encourage their patients to disclose their distress, and have shorter consultations, in order to gain time and energy. This may result in less psychological evaluations of patients' complaints. Methods We used 1890 videotaped consultations from a nationally representative sample of 126 Dutch GPs to analyse GPs' communication and the duration of their consultations. Burnout was subdivided into emotional exhaustion, depersonalisation and reduced accomplishment. Multilevel regression analyses were used to investigate which subgroups of GPs differed significantly. Results GPs with feelings of exhaustion or dissatisfaction with the available time have longer consultations compared to GPs without these feelings. Exhausted GPs, and GPs with feelings of depersonalisation, talk more about psychological or social topics in their consultations. GPs with feelings of reduced accomplishment are an exception: they communicate less affectively, are less patient-centred and have less eye contact with their patients compared to GPs without reduced accomplishment. We found no relationship between GPs' feelings of burnout or dissatisfaction with the available time and their psychological evaluations of patients' problems. Conclusion GPs' feelings of burnout or dissatisfaction with the time available for their patients do not obstruct their diagnosis and awareness of patients' psychological problems. On the contrary, GPs with high levels of exhaustion or depersonalisation, and GPs who are dissatisfied with the

  17. Bug City: Beetles [Videotape].

    Science.gov (United States)

    1998

    "Bug City" is a video series created to help children learn about insects and other small critters. All aspects of bug life are touched upon including body structure, food, habitat, life cycle, mating habits, camouflage, mutualism (symbiosis), adaptations, social behavior, and more. Each program features dramatic microscopic photography,…

  18. Weather Fundamentals: Meteorology. [Videotape].

    Science.gov (United States)

    1998

    The videos in this educational series, for grades 4-7, help students understand the science behind weather phenomena through dramatic live-action footage, vivid animated graphics, detailed weather maps, and hands-on experiments. This episode (23 minutes) looks at how meteorologists gather and interpret current weather data collected from sources…

  19. Secure surveillance videotapes

    Energy Technology Data Exchange (ETDEWEB)

    Resnik, W.M.; Kadner, S.P. [Aquila Technologies Group, Inc., Albuquerque, NM (United States); Olsen, R.; Chitumbo, K. [International Atomic Energy Agency, Vienna (Austria); Pepper, S. [International Safeguards Project Office, Vienna (Austria)

    1995-12-31

    With assistance from the US Program for Technical Assistance to IAEA Safeguards (POTAS), Aquila Technologies Group developed the Tamper-Resistant Analog Media (TRAM-1000) system to provide standard VHS surveillance video tapes with an enhanced tamper-indicating capability. This project represents further implementation of the partnership approach in facilities including light water reactors with MOX facilities. These facilities use Uniplex Digiquad system video tapes. The partnership approach ensures that one organization can exchange the tapes in a machine without the presence of the other, without losing continuity of information. The TRAM-1000 system development project was accomplished in two stages. In the first stage of the project, the original system delivered to the IAEA, consists of three parts: (1) the tamper detection unit, (2) a specially augmented VHS video tape, and (3) an HP-95 reader. The tamper detection unit houses a VACOSS active fiber-optic seal and an electronic identification tag (E-TAG) reader. In the second stage of the project, the original TRAM-1000 was modified to its current design based on agency input. After delivery of the original TRAM-1000 system to the IAEA, it was reviewed by inspectors. The inspectors felt that the initial system`s tape storage/transport method could be simplified. Rather than threading the fiber through the tape spindles, the inspectors suggested that the tape be placed in a bag capable of being sealed. Also, a more flexible fiber-optic cable was recommended. As a result of these suggestions, Aquila developed a tamper-proof bag specifically for holding a surveillance video tape and sealable with a VACOSS fiber optical seal.

  20. Details acquired from medical history and patients' experience of empathy--two sides of the same coin.

    Science.gov (United States)

    Ohm, Friedemann; Vogel, Daniela; Sehner, Susanne; Wijnen-Meijer, Marjo; Harendza, Sigrid

    2013-05-09

    History taking and empathetic communication are two important aspects in successful physician-patient interaction. Gathering important information from the patient's medical history is needed for effective clinical decision making while empathy is relevant for patient satisfaction. We wanted to investigate whether medical students near graduation are able to combine both skills as required in daily medical practice. Thirty near graduates from Hamburg Medical School participated in an assessment for clinical competences including a consultation hour with five standardized patients. Each patient interview was videotaped and standardized patients rated participants with the CARE questionnaire for consultation and relational empathy. All videotaped interviews were rated with a checklist based on the number of important medical aspects for each case. Data were analysed with the linear mixed model to correct for random effects. Regression analysis was performed to look for correlations between the number of questions asked by a participant and their respective empathy rating. Of the 123 aspects that could have been gathered in total, students only requested 56.4% (95% CI 53.5-59.3). While no difference between male and female participants was found, a significant difference (pcommunication are two completely separate sides of the coin of history taking. While both skills have to be acquired during medical school training with particular focus on their respective learning objectives, medical students need to be provided with additional learning and feedback opportunities where they can be observed exercising both skills combined as required in physicians' daily practice.

  1. Kiropraktikkens historie i Danmark

    DEFF Research Database (Denmark)

    Jørgensen, Per

    Bogen er den første samlede, forskningsbaserede fremstilling om kiropraktikkens danske historie. Den har udblik til kiropraktikkens historie i USA.......Bogen er den første samlede, forskningsbaserede fremstilling om kiropraktikkens danske historie. Den har udblik til kiropraktikkens historie i USA....

  2. Microforms and Sport History.

    Science.gov (United States)

    Levine, Peter

    1986-01-01

    Explores the importance of sport history as it reflects the social and cultural history of the United States. Discussion covers the various sport history materials that are available in microform, including the Spalding Collection, twentieth-century microfilm sources, and sports and social history (Sports Periodicals microfilm series). (EJS)

  3. What Is Literary "History"?

    Science.gov (United States)

    Harris, Wendell V.

    1994-01-01

    Examines the meaning of the word "history" as used in the common phrase "literary history" by critics and scholars. Asserts the differences between historical scholarship and literary history. Argues that the grounding activity of literary history is insulated from the relativism insisted upon by poststructuralist theorizing.…

  4. The influence of agitation sequence and ionic strength on in vitro drug release from hypromellose (E4M and K4M) ER matrices--the use of the USP III apparatus.

    Science.gov (United States)

    Asare-Addo, Kofi; Kaialy, Waseem; Levina, Marina; Rajabi-Siahboomi, Ali; Ghori, Mohammed U; Supuk, Enes; Laity, Peter R; Conway, Barbara R; Nokhodchi, Ali

    2013-04-01

    Theophylline extended release (ER) matrices containing hypromellose (hydroxypropyl methylcellulose (HPMC) E4M and K4M were evaluated in media with a pH range of 1.2-7.5, using an automated USP type III, Bio-Dis dissolution apparatus. The objectives of this study were to evaluate the effects of systematic agitation, ionic strength and pH on the release of theophylline from the gel forming hydrophilic polymeric matrices with different methoxyl substitution levels. Tribo-electric charging of hypromellose, theophylline and their formulated blends containing E4M and K4M grades has been characterised, along with quantitative observations of flow, compression behaviour and particle morphology. Agitations were studied at 5, 10, 15, 20, 25, 30 dips per minute (dpm) and also in the ascending and descending order in the dissolution vials. The ionic concentration strength of the media was also varied over a range of 0-0.4M to simulate the gastrointestinal fed and fasted states and various physiological pH conditions. To study the effect of ionic strength on the hydrophilic matrices, agitation was set at 20 dpm. The charge results on individual components imply that the positively charged particles have coupled with the negatively charged particles to form a stable ordered mixture which is believed to result in a more homogeneous and stable system. The particle shape analysis showed the HPMC K4M polymer to have a more irregular morphology and a rougher surface texture in comparison to the HPMC E4M polymer, possibly a contributory factor to the gelation process. The results showed gelation occurred quicker for the K4M tablet matrices. Drug release increased with increased agitation. This was more pronounced for the E4M tablet matrices. The ionic strength also had more of an effect on the drug release from the E4M matrices. The experiments highlighted the resilience of the K4M matrices in comparison with the E4M matrices. The results thus show that despite similar viscosities of

  5. GmPHD5 acts as an important regulator for crosstalk between histone H3K4 di-methylation and H3K14 acetylation in response to salinity stress in soybean

    Directory of Open Access Journals (Sweden)

    Wu Tao

    2011-12-01

    Full Text Available Abstract Background Accumulated evidence suggest that specific patterns of histone posttranslational modifications (PTMs and their crosstalks may determine transcriptional outcomes. However, the regulatory mechanisms of these "histone codes" in plants remain largely unknown. Results In this study, we demonstrate for the first time that a salinity stress inducible PHD (plant homeodomain finger domain containing protein GmPHD5 can read the "histone code" underlying the methylated H3K4. GmPHD5 interacts with other DNA binding proteins, including GmGNAT1 (an acetyl transferase, GmElongin A (a transcription elongation factor and GmISWI (a chromatin remodeling protein. Our results suggest that GmPHD5 can recognize specific histone methylated H3K4, with preference to di-methylated H3K4. Here, we illustrate that the interaction between GmPHD5 and GmGNAT1 is regulated by the self-acetylation of GmGNAT1, which can also acetylate histone H3. GmGNAT1 exhibits a preference toward acetylated histone H3K14. These results suggest a histone crosstalk between methylated H3K4 and acetylated H3K14. Consistent to its putative roles in gene regulation under salinity stress, we showed that GmPHD5 can bind to the promoters of some confirmed salinity inducible genes in soybean. Conclusion Here, we propose a model suggesting that the nuclear protein GmPHD5 is capable of regulating the crosstalk between histone methylation and histone acetylation of different lysine residues. Nevertheless, GmPHD5 could also recruit chromatin remodeling factors and transcription factors of salt stress inducible genes to regulate their expression in response to salinity stress.

  6. FOXP3 Orchestrates H4K16 Acetylation and H3K4 Tri-Methylation for Activation of Multiple Genes through Recruiting MOF and Causing Displacement of PLU-1

    Science.gov (United States)

    Katoh, Hiroto; Qin, Zhaohui S.; Liu, Runhua; Wang, Lizhong; Li, Weiquan; Li, Xiangzhi; Wu, Lipeng; Du, Zhanwen; Lyons, Robert; Liu, Chang-Gong; Liu, Xiuping; Dou, Yali; Zheng, Pan; Liu, Yang

    2011-01-01

    SUMMARY Both H4K16 acetylation and H3K4 tri-methylation are required for gene activation. However, it is still largely unclear how these modifications are orchestrated by transcriptional factors. Here we analyzed the mechanism of the transcriptional activation by FOXP3, an X-linked suppressor of autoimmune diseases and cancers. FOXP3 binds near transcriptional start sites of its target genes. By recruiting MOF and displacing histone H3K4 demethylase PLU-1, FOXP3 increases both H4K16 acetylation and H3K4 tri-methylation at the FOXP3-associated chromatins of multiple FOXP3-activated genes. RNAi-mediated silencing of MOF reduced both gene activation and tumor suppression by FOXP3, while both somatic mutations in clinical cancer samples and targeted mutation of FOXP3 in mouse prostate epithelial disrupted nuclear localization of MOF. Our data demonstrate a pull-push model in which a single transcription factor orchestrates two epigenetic alterations necessary for gene activation and provide a mechanism for somatic inactivation of the FOXP3 protein function in cancer cells. PMID:22152480

  7. AMPA, NMDA and kainate glutamate receptor subunits are expressed in human peripheral blood mononuclear cells (PBMCs) where the expression of GluK4 is altered by pregnancy and GluN2D by depression in pregnant women.

    Science.gov (United States)

    Bhandage, Amol K; Jin, Zhe; Hellgren, Charlotte; Korol, Sergiy V; Nowak, Krzysztof; Williamsson, Louise; Sundström-Poromaa, Inger; Birnir, Bryndis

    2017-04-15

    The amino acid glutamate opens cation permeable ion channels, the iGlu receptors. These ion channels are abundantly expressed in the mammalian brain where glutamate is the main excitatory neurotransmitter. The neurotransmitters and their receptors are being increasingly detected in the cells of immune system. Here we examined the expression of the 18 known subunits of the iGlu receptors families; α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), kainate, N-methyl-d-aspartate (NMDA) and delta in human peripheral blood mononuclear cells (PBMCs). We compared the expression of the subunits between four groups: men, non-pregnant women, healthy pregnant women and depressed pregnant women. Out of 18 subunits of the iGlu receptors, mRNAs for 11 subunits were detected in PBMCs from men and non-pregnant women; AMPA: GluA3, GluA4, kainate: GluK2, GluK4, GluK5, NMDA: GluN1, GluN2C, GluN2D, GluN3A, GluN3B, and delta: GluD1. In the healthy and the depressed pregnant women, in addition, the delta GluD2 subunit was identified. The mRNAs for GluK4, GluK5, GluN2C and GluN2D were expressed at a higher level than other subunits. Gender, pregnancy or depression during pregnancy altered the expression of GluA3, GluK4, GluN2D, GluN3B and GluD1 iGlu subunit mRNAs. The greatest changes recorded were the lower GluA3 and GluK4 mRNA levels in pregnant women and the higher GluN2D mRNA level in healthy but not in depressed pregnant women as compared to non-pregnant individuals. Using subunit specific antibodies, the GluK4, GluK5, GluN1, GluN2C and GluN2D subunit proteins were identified in the PBMCs. The results show expression of specific iGlu receptor subunit in the PBMCs and support the idea of physiology-driven changes of iGlu receptors subtypes in the immune cells. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Arabidopsis AL PHD-PRC1 complexes promote seed germination through H3K4me3-to-H3K27me3 chromatin state switch in repression of seed developmental genes.

    Science.gov (United States)

    Molitor, Anne Marie; Bu, Zhongyuan; Yu, Yu; Shen, Wen-Hui

    2014-01-01

    Seed germination and subsequent seedling growth define crucial steps for entry into the plant life cycle. For those events to take place properly, seed developmental genes need to be silenced whereas vegetative growth genes are activated. Chromatin structure is generally known to play crucial roles in gene transcription control. However, the transition between active and repressive chromatin states during seed germination is still poorly characterized and the underlying molecular mechanisms remain largely unknown. Here we identified the Arabidopsis PHD-domain H3K4me3-binding ALFIN1-like proteins (ALs) as novel interactors of the Polycomb Repressive Complex 1 (PRC1) core components AtBMI1b and AtRING1a. The interactions were confirmed by diverse in vitro and in vivo assays and were shown to require the AL6 N-terminus containing PAL domain conserved in the AL family proteins and the AtRING1a C-terminus containing RAWUL domain conserved in animal and plant PRC1 ring-finger proteins (including AtRNIG1a/b and AtBMI1a/b). By T-DNA insertion mutant analysis, we found that simultaneous loss of AL6 and AL7 as well as loss of AtBMI1a and AtBMI1b retards seed germination and causes transcriptional derepression and a delayed chromatin state switch from H3K4me3 to H3K27me3 enrichment of several seed developmental genes (e.g. ABI3, DOG1, CRU3, CHO1). We found that AL6 and the PRC1 H3K27me3-reader component LHP1 directly bind at ABI3 and DOG1 loci. In light of these data, we propose that AL PHD-PRC1 complexes, built around H3K4me3, lead to a switch from the H3K4me3-associated active to the H3K27me3-associated repressive transcription state of seed developmental genes during seed germination. Our finding of physical interactions between PHD-domain proteins and PRC1 is striking and has important implications for understanding the connection between the two functionally opposite chromatin marks: H3K4me3 in activation and H3K27me3 in repression of gene transcription.

  9. Arabidopsis AL PHD-PRC1 complexes promote seed germination through H3K4me3-to-H3K27me3 chromatin state switch in repression of seed developmental genes.

    Directory of Open Access Journals (Sweden)

    Anne Marie Molitor

    2014-01-01

    Full Text Available Seed germination and subsequent seedling growth define crucial steps for entry into the plant life cycle. For those events to take place properly, seed developmental genes need to be silenced whereas vegetative growth genes are activated. Chromatin structure is generally known to play crucial roles in gene transcription control. However, the transition between active and repressive chromatin states during seed germination is still poorly characterized and the underlying molecular mechanisms remain largely unknown. Here we identified the Arabidopsis PHD-domain H3K4me3-binding ALFIN1-like proteins (ALs as novel interactors of the Polycomb Repressive Complex 1 (PRC1 core components AtBMI1b and AtRING1a. The interactions were confirmed by diverse in vitro and in vivo assays and were shown to require the AL6 N-terminus containing PAL domain conserved in the AL family proteins and the AtRING1a C-terminus containing RAWUL domain conserved in animal and plant PRC1 ring-finger proteins (including AtRNIG1a/b and AtBMI1a/b. By T-DNA insertion mutant analysis, we found that simultaneous loss of AL6 and AL7 as well as loss of AtBMI1a and AtBMI1b retards seed germination and causes transcriptional derepression and a delayed chromatin state switch from H3K4me3 to H3K27me3 enrichment of several seed developmental genes (e.g. ABI3, DOG1, CRU3, CHO1. We found that AL6 and the PRC1 H3K27me3-reader component LHP1 directly bind at ABI3 and DOG1 loci. In light of these data, we propose that AL PHD-PRC1 complexes, built around H3K4me3, lead to a switch from the H3K4me3-associated active to the H3K27me3-associated repressive transcription state of seed developmental genes during seed germination. Our finding of physical interactions between PHD-domain proteins and PRC1 is striking and has important implications for understanding the connection between the two functionally opposite chromatin marks: H3K4me3 in activation and H3K27me3 in repression of gene transcription.

  10. The effects of videotape modeling and daily feedback on residential electricity conservation, home temperature and humidity, perceived comfort, and clothing worn: Winter and summer.

    Science.gov (United States)

    Winett, R A; Hatcher, J W; Fort, T R; Leckliter, I N; Love, S Q; Riley, A W; Fishback, J F

    1982-01-01

    Two studies were conducted in all-electric townhouses and apartments in the winter (N = 83) and summer (N = 54) to ascertain how energy conservation strategies focusing on thermostat change and set-backs and other low-cost/no-cost approaches would affect overall electricity use and electricity used for heating and cooling, the home thermal environment, the perceived comfort of participants, and clothing that was worn. The studies assessed the effectiveness of videotape modeling programs that demonstrated these conservation strategies when used alone or combined with daily feedback on electricity use. In the winter, the results indicated that videotape modeling and/or feedback were effective relative to baseline and to a control group in reducing overall electricity use by about 15% and electricity used for heating by about 25%. Hygrothermographs, which accurately and continuously recorded temperature and humidity in the homes, indicated that participants were able to live with no reported loss in comfort and no change in attire at a mean temperature of about 62 degrees F when home and about 59 degrees F when asleep. The results were highly discrepant with prior laboratory studies indicating comfort at 75 degrees F with the insulation value of the clothing worn by participants in this study. In the summer, a combination of strategies designed to keep a home cool with minimal or no air conditioning, in conjunction with videotape modeling and/or daily feedback, resulted in overall electricity reductions of about 15% with reductions on electricity for cooling of about 34%, but with feedback, and feedback and modeling more effective than modeling alone. Despite these electricity savings, hygrothermograph recordings indicated minimal temperature change in the homes, with no change in perceived comfort or clothing worn. The results are discussed in terms of discrepancies with laboratory studies, optimal combinations of video-media and personal contact to promote behavior

  11. History of Bioterrorism: Botulism

    Medline Plus

    Full Text Available ... Education What's New Emergency Preparedness and You Video: "The History of Bioterrorism" Recommend on Facebook Tweet Share ... or can be used as bioterrorist weapons. Watch the Complete Program "The History of Bioterroism" (26 min ...

  12. "Hillary - en god historie"

    DEFF Research Database (Denmark)

    Bjerre, Thomas Ærvold

    2007-01-01

    Anmeldelse af Carl Bernsteins Hillary Rodham Clinton og Michael Ehrenreichs Hillary - En amerikansk historie Udgivelsesdato: 15. november......Anmeldelse af Carl Bernsteins Hillary Rodham Clinton og Michael Ehrenreichs Hillary - En amerikansk historie Udgivelsesdato: 15. november...

  13. History of Bioterrorism: Botulism

    Science.gov (United States)

    ... Guide Reaching At-Risk Populations MedCon Video: "The History of Bioterrorism" Recommend on Facebook Tweet Share Compartir ... as bioterrorist weapons. Watch the Complete Program "The History of Bioterroism" (26 min 38 sec) Watch Specific ...

  14. History of Bioterrorism: Botulism

    Medline Plus

    Full Text Available ... What's New Emergency Preparedness and You Video: "The History of Bioterrorism" Recommend on Facebook Tweet Share Compartir ... as bioterrorist weapons. Watch the Complete Program "The History of Bioterroism" (26 min 38 sec) Watch Specific ...

  15. History of Bioterrorism: Botulism

    Medline Plus

    Full Text Available ... Education What's New Emergency Preparedness and You Video: "The History of Bioterrorism" Recommend on Facebook Tweet Share ... or can be used as bioterrorist weapons. Watch the Complete Program "The History of Bioterroism" (26 min ...

  16. HAD Oral History Project

    Science.gov (United States)

    Holbrook, Jarita

    2014-01-01

    The Historical Astronomy Division is the recipient of an American Institute of Physics Neils Bohr Library Grant for Oral History. HAD has assembled a team of volunteers to conduct oral history interviews since May 2013. Each oral history interview varies in length between two and six hours. This presentation is an introduction to the HAD Oral History Project and the activities of the team during the first six months of the grant.

  17. Canadian petroleum history bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Cass, D.

    2003-09-27

    The Petroleum History Bibliography includes a list of more than 2,000 publications that record the history of the Canadian petroleum industry. The list includes books, theses, films, audio tapes, published articles, company histories, biographies, autobiographies, fiction, poetry, humour, and an author index. It was created over a period of several years to help with projects at the Petroleum History Society. It is an ongoing piece of work, and as such, invites comments and additions.

  18. Towards a European History

    NARCIS (Netherlands)

    H. van Dijk (Henk)

    2000-01-01

    textabstractAlthough historical writing is a profession with a long tradition, history as an academic discipline is strongly related to the development of the nation state in the nineteenth century. Notwithstanding specialisations like e.g. cultural history and social and economic history put less e

  19. Modern History of Tibet

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Authored by Xu Guangzhi, this book is a subsidiary project of Research Into Traditional Culture and History (of the PRC Ministry of Education) conducted by China Tibetology Research Institute of Tibet University. The book combines modern history of Tibet with modern history of China as a whole. It tells the close ties between various members of the Chinese nation.

  20. Conducting the Medical History

    Science.gov (United States)

    Finkel, Martin A.; Alexander, Randell A.

    2011-01-01

    A key portion of the medical evaluation of child sexual abuse is the medical history. This differs from interviews or histories obtained by other professionals in that it is focuses more on the health and well-being of the child. Careful questions should be asked about all aspects of the child's medical history by a skilled, compassionate,…

  1. Vamos juntos a la escuela: Consejos para ayudar a los padres a asegurar el exito academico de sus hijos (Let's Go to School Together: Tips To Help Parents Ensure Their Children's Educational Success). [Videotape Kit].

    Science.gov (United States)

    Information Resource Center (ED), Washington, DC.

    Noting research showing that when parents are involved in their children's learning, students achieve more and are less likely to drop out of school, this videotape kit offers Spanish-speaking parents tips to help them become involved in their children's learning, from infancy through adolescence. The kit is intended for use in schools,…

  2. History of Science and History of Philologies.

    Science.gov (United States)

    Daston, Lorraine; Most, Glenn W

    2015-06-01

    While both the sciences and the humanities, as currently defined, may be too heterogeneous to be encompassed within a unified historical framework, there is good reason to believe that the history of science and the history of philologies both have much to gain by joining forces. This collaboration has already yielded striking results in the case of the history of science and humanist learning in early modern Europe. This essay argues that first, philology and at least some of the sciences (e.g., astronomy) remained intertwined in consequential ways well into the modern period in Western cultures; and second, widening the scope of inquiry to include other philological traditions in non-Western cultures offers rich possibilities for a comparative history of learned practices. The focus on practices is key; by shifting the emphasis from what is studied to how it is studied, deep commonalities emerge among disciplines--and intellectual traditions--now classified as disparate.

  3. Diet History Questionnaire: Database Revision History

    Science.gov (United States)

    The following details all additions and revisions made to the DHQ nutrient and food database. This revision history is provided as a reference for investigators who may have performed analyses with a previous release of the database.

  4. Diet History Questionnaire: Database Revision History

    Science.gov (United States)

    The following details all additions and revisions made to the DHQ nutrient and food database. This revision history is provided as a reference for investigators who may have performed analyses with a previous release of the database.

  5. History of mathematics and history of science

    OpenAIRE

    Mann, Tony

    2011-01-01

    This essay argues that the diversity of the history of mathematics community in the United Kingdom has influenced the development of the subject and is a significant factor behind the different concerns often evident in work on the history of mathematics when compared with that of historians of science. The heterogeneous nature of the community, which includes many who are not specialist historians, and the limited opportunities for academic\\ud careers open to practitioners have had a profoun...

  6. Portraying User Interface History

    DEFF Research Database (Denmark)

    Jørgensen, Anker Helms

    2008-01-01

    in that they largely address prevailing UI techno­logies, and thirdly history from above in that they focus on the great deeds of the visionaries. The paper then compares this state-of-art in UI history to the much more mature fields history of computing and history of technology. Based hereon, some speculations......The user interface is coming of age. Papers adressing UI history have appeared in fair amounts in the last 25 years. Most of them address particular aspects such as an in­novative interface paradigm or the contribution of a visionary or a research lab. Contrasting this, papers addres­sing UI...... history at large have been sparse. However, a small spate of publications appeared recently, so a reasonable number of papers are available. Hence this work-in-progress paints a portrait of the current history of user interfaces at large. The paper first describes a theoretical framework recruited from...

  7. Quantitative analysis of ChIP-seq data uncovers dynamic and sustained H3K4me3 and H3K27me3 modulation in cancer cells under hypoxia.

    Science.gov (United States)

    Adriaens, Michiel E; Prickaerts, Peggy; Chan-Seng-Yue, Michelle; van den Beucken, Twan; Dahlmans, Vivian E H; Eijssen, Lars M; Beck, Timothy; Wouters, Bradly G; Voncken, Jan Willem; Evelo, Chris T A

    2016-01-01

    A comprehensive assessment of the epigenetic dynamics in cancer cells is the key to understanding the molecular mechanisms underlying cancer and to improving cancer diagnostics, prognostics and treatment. By combining genome-wide ChIP-seq epigenomics and microarray transcriptomics, we studied the effects of oxygen deprivation and subsequent reoxygenation on histone 3 trimethylation of lysine 4 (H3K4me3) and lysine 27 (H3K27me3) in a breast cancer cell line, serving as a model for abnormal oxygenation in solid tumors. A priori, epigenetic markings and gene expression levels not only are expected to vary greatly between hypoxic and normoxic conditions, but also display a large degree of heterogeneity across the cell population. Where traditionally ChIP-seq data are often treated as dichotomous data, the model and experiment here necessitate a quantitative, data-driven analysis of both datasets. We first identified genomic regions with sustained epigenetic markings, which provided a sample-specific reference enabling quantitative ChIP-seq data analysis. Sustained H3K27me3 marking was located around centromeres and intergenic regions, while sustained H3K4me3 marking is associated with genes involved in RNA binding, translation and protein transport and localization. Dynamic marking with both H3K4me3 and H3K27me3 (hypoxia-induced bivalency) was found in CpG-rich regions at loci encoding factors that control developmental processes, congruent with observations in embryonic stem cells. In silico-identified epigenetically sustained and dynamic genomic regions were confirmed through ChIP-PCR in vitro, and obtained results are corroborated by published data and current insights regarding epigenetic regulation.

  8. Se14, encoding a JmjC domain-containing protein, plays key roles in long-day suppression of rice flowering through the demethylation of H3K4me3 of RFT1.

    Directory of Open Access Journals (Sweden)

    Takayuki Yokoo

    Full Text Available Floral transition from the vegetative to the reproductive growth phase is a major change in the plant life cycle and a key factor in reproductive success. In rice (Oryza sativa L., a facultative short-day plant, numerous flowering time and flower formation genes that control floral transition have been identified and their physiological effects and biochemical functions have been clarified. In the present study, we used a Se14-deficient mutant line (HS112 and other flowering mutant lines to investigate the photoperiodic response, chromosomal location and function in the photoperiod sensitivity of the Se14 gene. We also studied the interactive effects of this locus with other crucial flowering time genes. We found that Se14 is independent of the known photoperiod-sensitive genes, such as Hd1 and Ghd7, and is identical to Os03g0151300, which encodes a Jumonji C (JmjC domain-containing protein. Expression analysis revealed that the expressions of RFT1, a floral initiator known as a "florigen-like gene", and Ehd1 were up-regulated in HS112, whereas this up-regulation was not observed in the original variety of 'Gimbozu'. ChIP assays of the methylation states of histone H3 at lysine 4 (H3K4 revealed that the trimethylated H3K4 in the promoter region of the RFT1 chromatin was significantly increased in HS112. We conclude that Se14 is a novel photoperiod-sensitivity gene that has a suppressive effect on floral transition (flowering time under long day-length conditions through the modification of chromatin structure by H3K4me3 demethylation in the promoter region of RFT1.

  9. History of mathematics and history of science.

    Science.gov (United States)

    Mann, Tony

    2011-09-01

    This essay argues that the diversity of the history of mathematics community in the United Kingdom has influenced the development of the subject and is a significant factor behind the different concerns often evident in work on the history of mathematics when compared with that of historians of science. The heterogeneous nature of the community, which includes many who are not specialist historians, and the limited opportunities for academic careers open to practitioners have had a profound effect on the discipline, leading to a focus on elite mathematics and great mathematicians. More recently, reflecting earlier developments in the history of science, an increased interest in the context and culture of the practice of mathematics has become evident.

  10. Marine Environmental History

    DEFF Research Database (Denmark)

    Poulsen, Bo

    2012-01-01

    This essay provides an overview of recent trends in the historiography of marine environmental history, a sub-field of environmental history which has grown tremendously in scope and size over the last c. 15 years. The object of marine environmental history is the changing relationship between...... human society and natural marine resources. Within this broad topic, several trends and objectives are discernable. The essay argue that the so-called material marine environmental history has its main focus on trying to reconstruct the presence, development and environmental impact of past fisheries...... and whaling operations. This ambition often entails a reconstruction also of how marine life has changed over time. The time frame rages from Paleolithicum to the present era. The field of marine environmental history also includes a more culturally oriented environmental history, which mainly has come...

  11. Ranking Economic History Journals

    DEFF Research Database (Denmark)

    Di Vaio, Gianfranco; Weisdorf, Jacob Louis

    This study ranks - for the first time - 12 international academic journals that have economic history as their main topic. The ranking is based on data collected for the year 2007. Journals are ranked using standard citation analysis where we adjust for age, size and self-citation of journals. We...... also compare the leading economic history journals with the leading journals in economics in order to measure the influence on economics of economic history, and vice versa. With a few exceptions, our results confirm the general idea about what economic history journals are the most influential...... for economic history, and that, although economic history is quite independent from economics as a whole, knowledge exchange between the two fields is indeed going on....

  12. Counting coalescent histories.

    Science.gov (United States)

    Rosenberg, Noah A

    2007-04-01

    Given a species tree and a gene tree, a valid coalescent history is a list of the branches of the species tree on which coalescences in the gene tree take place. I develop a recursion for the number of valid coalescent histories that exist for an arbitrary gene tree/species tree pair, when one gene lineage is studied per species. The result is obtained by defining a concept of m-extended coalescent histories, enumerating and counting these histories, and taking the special case of m = 1. As a sum over valid coalescent histories appears in a formula for the probability that a random gene tree evolving along the branches of a fixed species tree has a specified labeled topology, the enumeration of valid coalescent histories can considerably reduce the effort required for evaluating this formula.

  13. Family Health History and Diabetes

    Science.gov (United States)

    ... Diabetes Diabetes Risk Test Family Health History Quiz Family Health History Quiz Family health history is an ... health problems. Four Questions You Should Ask Your Family About Diabetes & Family Health History Knowing your family ...

  14. Enhanced photoelectrocatalytic decomposition of copper cyanide complexes and simultaneous recovery of copper with a Bi2MoO6 electrode under visible light by EDTA/K4P2O7.

    Science.gov (United States)

    Zhao, Xu; Zhang, Juanjuan; Qiao, Meng; Liu, Huijuan; Qu, Jiuhui

    2015-04-07

    Simultaneous photoelectrocatalytic (PEC) oxidation of cyanides and recovery of copper in a PEC reactor with a Bi(2)MoO(6) photoanode was investigated at alkaline conditions under visible light irradiation. The surface variation of the Bi(2)MoO(6) photoanode and titanium cathode was characterized. The Cu mass distribution onto the anode, in the solution, and onto the cathode was fully investigated. In the individual PEC oxidation of copper cyanides, the formation of a black copper oxide on the anode occurred. By keeping the initial cyanide concentration at 0.01 mM, the effect of EDTA/K(4)P(2)O(7) was examined at different molar ratios of EDTA/K(4)P(2)O(7) to cyanide. It was indicated that the oxidation of cyanides increased and simultaneous copper electrodeposition with zero value onto the cathode was feasible at pH 11. Under the optimal conditions, the total cyanide concentration was lowered from 250 to 5.0 mg/L, and the Cu recovery efficiency deposited onto the cathode was higher than 90%. Cyanate was the only product. The role of the photogenerated hole in the oxidation of cyanide ions was confirmed.

  15. Cooperative Station History Forms

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Various forms, photographs and correspondence documenting the history of Cooperative station instrumentation, location changes, inspections, and...

  16. Ranking Economic History Journals

    DEFF Research Database (Denmark)

    Di Vaio, Gianfranco; Weisdorf, Jacob Louis

    This study ranks - for the first time - 12 international academic journals that have economic history as their main topic. The ranking is based on data collected for the year 2007. Journals are ranked using standard citation analysis where we adjust for age, size and self-citation of journals. We...... also compare the leading economic history journals with the leading journals in economics in order to measure the influence on economics of economic history, and vice versa. With a few exceptions, our results confirm the general idea about what economic history journals are the most influential...

  17. Ranking economic history journals

    DEFF Research Database (Denmark)

    Di Vaio, Gianfranco; Weisdorf, Jacob Louis

    2010-01-01

    This study ranks-for the first time-12 international academic journals that have economic history as their main topic. The ranking is based on data collected for the year 2007. Journals are ranked using standard citation analysis where we adjust for age, size and self-citation of journals. We also...... compare the leading economic history journals with the leading journals in economics in order to measure the influence on economics of economic history, and vice versa. With a few exceptions, our results confirm the general idea about what economic history journals are the most influential for economic...

  18. Public and popular history

    CERN Document Server

    De Groot, Jerome

    2013-01-01

    This interdisciplinary collection considers public and popular history within a global framework, seeking to understand considerations of local, domestic histories and the ways they interact with broader discourses. Grounded in particular local and national situations, the book addresses the issues associated with popular history in a globalised cultural world, such as: how the study of popular history might work in the future; new ways in which the terms 'popular' and 'public' might inform one another and nuance scholarship; transnational, intercultural models of 'pastness'; cultural translat

  19. K4W2OCl10

    Science.gov (United States)

    Villars, P.; Cenzual, K.; Daams, J.; Gladyshevskii, R.; Shcherban, O.; Dubenskyy, V.; Kuprysyuk, V.; Savysyuk, I.; Zaremba, R.

    This document is part of Subvolume A10 'Structure Types. Part 10: Space Groups (140) I4/mcm - (136) P42/mnm' of Volume 43 'Crystal Structures of Inorganic Compounds' of Landolt-Börnstein - Group III 'Condensed Matter'.

  20. Teaching Sport as History, History through Sport

    Science.gov (United States)

    Wheeler, Robert F.

    1978-01-01

    Describes an undergraduate history course based on two themes: sport as a reflection of society and sport as a socializing agent affecting society. The course focuses on sports and industrialization, traditional and modern sports, political and economic aspects of sport, and inequality and discrimination in sports. (Author/JK)

  1. A history of the histories of econometrics

    NARCIS (Netherlands)

    Boumans, M.; Dupont-Kieffer, A.

    2011-01-01

    Econometricians have from the start considered historical knowledge of their own discipline as reflexive knowledge useful for delineating their discipline, that is, for setting its disciplinary boundaries with respect to its aims, its methods, and its scientific values. As such, the histories writte

  2. O uso de filmagem em pesquisas qualitativas El uso de la filmación en investigaciones cualitativas The use of videotaping in qualitative research

    Directory of Open Access Journals (Sweden)

    Eliana Moreira Pinheiro

    2005-10-01

    soluciones se deben basar en acuerdos entre los investigadores y los participantes.Considering advancements in data collection methods, we explore the use of videotaping in qualitative research. This bibliographical study aims at developing reflections on the possibilities of using videotapes in research and at providing material to researchers. The video is used as an instrument of data collection and generation. We mention technical aspects, such as the utilization of a mobile or fixed camera. By means of the latter, the authors report their experience, emphasizing compliance with the neutrality principle and the possibility of editing the images obtained as a means of generating new data. The authors highlight that it was possible to detect contradictions between discourse and behavior through the use of videotaping and interviews. The authors also discuss the ethical principles set by CNS Resolution 196/96 and other ethical questions, whose solutions should be based on the agreement between researchers and subjects.

  3. History of Science

    Science.gov (United States)

    Oversby, John

    2010-01-01

    In this article, the author discusses why the history of science should be included in the science curriculum in schools. He also presents some opportunities that can come out of using historical contexts, and findings from a study assessing the place of history of science in readily available textbooks.

  4. History of Science

    Science.gov (United States)

    Oversby, John

    2010-01-01

    In this article, the author discusses why the history of science should be included in the science curriculum in schools. He also presents some opportunities that can come out of using historical contexts, and findings from a study assessing the place of history of science in readily available textbooks.

  5. History of Mathematics

    DEFF Research Database (Denmark)

    Hansen, Vagn Lundsgaard; Gray, Jeremy

    Volume 1 in Theme on "History of Mathematics", in "Encyclopedia of Life Support Systems (EOLSS), developed under the auspices of the UNESCO.......Volume 1 in Theme on "History of Mathematics", in "Encyclopedia of Life Support Systems (EOLSS), developed under the auspices of the UNESCO....

  6. Writing American Indian History

    Science.gov (United States)

    Noley, Grayson B.

    2008-01-01

    The purpose of this paper is to critique the manner in which history about American Indians has been written and propose a rationale for the rethinking of what we know about this subject. In particular, histories of education as regards the participation of American Indians is a subject that has been given scant attention over the years and when…

  7. Aggersborg through history

    DEFF Research Database (Denmark)

    2014-01-01

    Aggersborg's history from the time of the end of the circular fortress till the present day, with a focus on the late Viking Age and the Middle Ages......Aggersborg's history from the time of the end of the circular fortress till the present day, with a focus on the late Viking Age and the Middle Ages...

  8. History of Mathematics

    DEFF Research Database (Denmark)

    Hansen, Vagn Lundsgaard; Gray, Jeremy

    Volume 1 in Theme on "History of Mathematics", in "Encyclopedia of Life Support Systems (EOLSS), developed under the auspices of the UNESCO.......Volume 1 in Theme on "History of Mathematics", in "Encyclopedia of Life Support Systems (EOLSS), developed under the auspices of the UNESCO....

  9. Final report on EURAMET.M.P-K4.2010: Key and supplementary comparison of national pressure standards in the range 1 Pa to 15 kPa of absolute and gauge pressure

    Science.gov (United States)

    Krajíček, Zdeněk; Bergoglio, Mercede; Jousten, Karl; Otal, Pierre; Sabuga, Wladimir; Saxholm, Sari; Pražák, Dominik; Vičar, Martin

    2014-01-01

    This report describes a EURAMET comparison of five European National Metrology Institutes in low gauge and absolute pressure in gas (nitrogen), denoted as EURAMET.M.P-K4.2010. Its main intention is to state equivalence of the pressure standards, in particular those based on the technology of force-balanced piston gauges such as e.g. FRS by Furness Controls, UK and FPG8601 by DHI-Fluke, USA. It covers the range from 1 Pa to 15 kPa, both gauge and absolute. The comparison in absolute mode serves as a EURAMET Key Comparison which can be linked to CCM.P-K4 and CCM.P-K2 via PTB. The comparison in gauge mode is a supplementary comparison. The comparison was carried out from September 2008 till October 2012. The participating laboratories were the following: CMI, INRIM, LNE, MIKES, PTB-Berlin (absolute pressure 1 kPa and below) and PTB-Braunschweig (absolute pressure 1 kPa and above and gauge pressure). CMI was the pilot laboratory and provided a transfer standard for the comparison. This transfer standard was also the laboratory standard of CMI at the same time, which resulted in a unique and logistically difficult star comparison. Both in gauge and absolute pressures all the participating institutes successfully proved their equivalence with respect to the reference value and all also proved mutual bilateral equivalences in all the points. All the participating laboratories are also equivalent with the reference values of CCM.P-K4 and CCM.P-K2 in the relevant points. The comparison also proved the ability of FPG8601 to serve as a transfer standard. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  10. Regulation of Transcription of Nucleotide-Binding Leucine-Rich Repeat-Encoding Genes SNC1 and RPP4 via H3K4 Trimethylation1[C][W][OA

    Science.gov (United States)

    Xia, Shitou; Cheng, Yu Ti; Huang, Shuai; Win, Joe; Soards, Avril; Jinn, Tsung-Luo; Jones, Jonathan D.G.; Kamoun, Sophien; Chen, She; Zhang, Yuelin; Li, Xin

    2013-01-01

    Plant nucleotide-binding leucine-rich repeat (NB-LRR) proteins serve as intracellular sensors to detect pathogen effectors and trigger immune responses. Transcription of the NB-LRR-encoding Resistance (R) genes needs to be tightly controlled to avoid inappropriate defense activation. How the expression of the NB-LRR R genes is regulated is poorly understood. The Arabidopsis (Arabidopsis thaliana) suppressor of npr1-1, constitutive 1 (snc1) mutant carries a gain-of-function mutation in a Toll/Interleukin1 receptor-like (TIR)-NB-LRR-encoding gene, resulting in the constitutive activation of plant defense responses. A snc1 suppressor screen identified modifier of snc1,9 (mos9), which partially suppresses the autoimmune phenotypes of snc1. Positional cloning revealed that MOS9 encodes a plant-specific protein of unknown function. Expression analysis showed that MOS9 is required for the full expression of TIR-NB-LRR protein-encoding RECOGNITION OF PERONOSPORA PARASITICA 4 (RPP4) and SNC1, both of which reside in the RPP4 cluster. Coimmunoprecipitation and mass spectrometry analyses revealed that MOS9 associates with the Set1 class lysine 4 of histone 3 (H3K4) methyltransferase Arabidopsis Trithorax-Related7 (ATXR7). Like MOS9, ATXR7 is also required for the full expression of SNC1 and the autoimmune phenotypes in the snc1 mutant. In atxr7 mutant plants, the expression of RPP4 is similarly reduced, and resistance against Hyaloperonospora arabidopsidis Emwa1 is compromised. Consistent with the attenuated expression of SNC1 and RPP4, trimethylated H3K4 marks are reduced around the promoters of SNC1 and RPP4 in mos9 plants. Our data suggest that MOS9 functions together with ATXR7 to regulate the expression of SNC1 and RPP4 through H3K4 methylation, which plays an important role in fine-tuning their transcription levels and functions in plant defense. PMID:23690534

  11. H2A.Z demarcates intergenic regions of the plasmodium falciparum epigenome that are dynamically marked by H3K9ac and H3K4me3.

    Directory of Open Access Journals (Sweden)

    Richárd Bártfai

    Full Text Available Epigenetic regulatory mechanisms and their enzymes are promising targets for malaria therapeutic intervention; however, the epigenetic component of gene expression in P. falciparum is poorly understood. Dynamic or stable association of epigenetic marks with genomic features provides important clues about their function and helps to understand how histone variants/modifications are used for indexing the Plasmodium epigenome. We describe a novel, linear amplification method for next-generation sequencing (NGS that allows unbiased analysis of the extremely AT-rich Plasmodium genome. We used this method for high resolution, genome-wide analysis of a histone H2A variant, H2A.Z and two histone H3 marks throughout parasite intraerythrocytic development. Unlike in other organisms, H2A.Z is a constant, ubiquitous feature of euchromatic intergenic regions throughout the intraerythrocytic cycle. The almost perfect colocalisation of H2A.Z with H3K9ac and H3K4me3 suggests that these marks are preferentially deposited on H2A.Z-containing nucleosomes. By performing RNA-seq on 8 time-points, we show that acetylation of H3K9 at promoter regions correlates very well with the transcriptional status whereas H3K4me3 appears to have stage-specific regulation, being low at early stages, peaking at trophozoite stage, but does not closely follow changes in gene expression. Our improved NGS library preparation procedure provides a foundation to exploit the malaria epigenome in detail. Furthermore, our findings place H2A.Z at the cradle of P. falciparum epigenetic regulation by stably defining intergenic regions and providing a platform for dynamic assembly of epigenetic and other transcription related complexes.

  12. KdmB, a Jumonji Histone H3 Demethylase, Regulates Genome-Wide H3K4 Trimethylation and Is Required for Normal Induction of Secondary Metabolism in Aspergillus nidulans.

    Directory of Open Access Journals (Sweden)

    Agnieszka Gacek-Matthews

    2016-08-01

    Full Text Available Histone posttranslational modifications (HPTMs are involved in chromatin-based regulation of fungal secondary metabolite biosynthesis (SMB in which the corresponding genes-usually physically linked in co-regulated clusters-are silenced under optimal physiological conditions (nutrient-rich but are activated when nutrients are limiting. The exact molecular mechanisms by which HPTMs influence silencing and activation, however, are still to be better understood. Here we show by a combined approach of quantitative mass spectrometry (LC-MS/MS, genome-wide chromatin immunoprecipitation (ChIP-seq and transcriptional network analysis (RNA-seq that the core regions of silent A. nidulans SM clusters generally carry low levels of all tested chromatin modifications and that heterochromatic marks flank most of these SM clusters. During secondary metabolism, histone marks typically associated with transcriptional activity such as H3 trimethylated at lysine-4 (H3K4me3 are established in some, but not all gene clusters even upon full activation. KdmB, a Jarid1-family histone H3 lysine demethylase predicted to comprise a BRIGHT domain, a zinc-finger and two PHD domains in addition to the catalytic Jumonji domain, targets and demethylates H3K4me3 in vivo and mediates transcriptional downregulation. Deletion of kdmB leads to increased transcription of about ~1750 genes across nutrient-rich (primary metabolism and nutrient-limiting (secondary metabolism conditions. Unexpectedly, an equally high number of genes exhibited reduced expression in the kdmB deletion strain and notably, this group was significantly enriched for genes with known or predicted functions in secondary metabolite biosynthesis. Taken together, this study extends our general knowledge about multi-domain KDM5 histone demethylases and provides new details on the chromatin-level regulation of fungal secondary metabolite production.

  13. Examining the content of weight, nutrition and physical activity advices provided by Dutch practice nurses in primary care: analysis of videotaped consultations.

    Science.gov (United States)

    van Dillen, S M E; Noordman, J; van Dulmen, S; Hiddink, G J

    2014-01-01

    To examine the content of Dutch practice nurses' (PNs') advices about weight, nutrition and physical activity to overweight and obese patients. A 100 videotaped real-life PN consultations (The Netherlands, 2010/2011) with overweight or obese patients were selected. An observational checklist was developed to assess frequency and content. Personalization of advices was scored, as also the guidelines on which PNs based their advices. Content analysis was used to identify different categories of advices. About one quarter of advices concerned weight, over two-thirds nutrition and one-third physical activity. Lose weight, eat less fat and be more physically active in general were the main categories for each type of advice. Despite high clarity of advices, lower scores were found for specificity and personalization. Very few nutrition advices were provided in combination with physical activity advices. Weight advices often related to the patient's complaint. PNs seldom set a concrete weight goal. Although benefits of physical activity were discussed, often no practical advices were provided about how to achieve this. Integrated lifestyle advice was not common: advices about nutrition and physical activity were fragmented throughout the consultation. Obesity prevention needs more emphasis in PNs' educational programs.

  14. A history of mathematics

    CERN Document Server

    Boyer, Carl B

    2011-01-01

    The updated new edition of the classic and comprehensive guide to the history of mathematics. For more than forty years, A History of Mathematics has been the reference of choice for those looking to learn about the fascinating history of humankind's relationship with numbers, shapes, and patterns. This revised edition features up-to-date coverage of topics such as Fermat's Last Theorem and the Poincaré Conjecture , in addition to recent advances in areas such as finite group theory and computer-aided proofs.: Distills thousands of years of mathematics into a single, approachable volume; Cover

  15. Science A history

    CERN Document Server

    Gribbin, John

    2002-01-01

    From award-winning science writer John Gribbin, "Science: A History" is the enthralling story of the men and women who changed the way we see the world, and the turbulent times they lived in. From Galileo, tried by the Inquisition for his ideas, to Newton, who wrote his rivals out of the history books; from Marie Curie, forced to work apart from male students for fear she might excite them, to Louis Agassiz, who marched his colleagues up a mountain to prove that the ice ages had occurred. Filled with pioneers, visionaries, eccentrics and madmen, this is the history of science as it has never been told before.

  16. Life History Approach

    DEFF Research Database (Denmark)

    Olesen, Henning Salling

    2015-01-01

    as in everyday life. Life histories represent lived lives past, present and anticipated future. As such they are interpretations of individuals’ experiences of the way in which societal dynamics take place in the individual body and mind, either by the individual him/herself or by another biographer. The Life...... History approach was developing from interpreting autobiographical and later certain other forms of language interactive material as moments of life history, i.e. it is basically a hermeneutic approach. Talking about a psycho-societal approach indicates the ambition of attacking the dichotomy...... of the social and the psychic, both in the interpretation procedure and in some main theoretical understandings of language, body and mind. My article will present the reflections on the use of life history based methodology in learning and education research as a kind of learning story of research work....

  17. Water Level Station History

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Images contain station history information for 175 stations in the National Water Level Observation Network (NWLON). The NWLON is a network of long-term,...

  18. History and Legacy

    Science.gov (United States)

    Mason, Diana S.

    2004-01-01

    The history of the computer usage in high school laboratories is discussed. Students learned scientific methods by acknowledging measurement errors, using significant digits, questioning their own results, and without doubts, they benefited from applying skill learned in mathematics classes.

  19. Brief history of dreams

    National Research Council Canada - National Science Library

    Rosselli, D

    2000-01-01

    Throughout history dreams have played a crucial role. Dreams have inspired great works of art, solved scientific problems and, because of the premonitory value attached to them, have influenced transcendental decisions...

  20. Oral history database

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Separately, each history provides an in depth view into the professional and personal lives of individual participants. Together, they have the power to illuminate...

  1. IUTAM a short history

    CERN Document Server

    Juhasz, Stephen

    2016-01-01

    This book presents extensive information related to the history of IUTAM. The initial chapters focus on IUTAM’s history and selected organizational aspects. Subsequent chapters provide extensive data and statistics, while the closing section showcases photos from all periods of the Union’s history. The history of IUTAM, the International Union on Theoretical and Applied Mechanics, began at a conference in 1922 in Innsbruck, Austria, where von Kármán put forward the idea of an international congress including the whole domain of applied mechanics. In 1946 IUTAM was then formally launched in Paris/France. IUTAM has since time organized more than 24 world congresses and 380 symposia, representing all fields of mechanics and highlighting advances by prominent international researchers. The efforts of IUTAM and its about 50 member countries serve to promote the mechanical sciences and the advancement of human society, addressing many key challenges. In this context, IUTAM preserves important traditions while...

  2. GBM Accreting Pulsar Histories

    Data.gov (United States)

    National Aeronautics and Space Administration — For each source we plot the history of pulse frequency and pulsed flux measured using the Fermi Gamma-Ray Burst Monitor (GBM) NaI detectors. For these measurements...

  3. Social Psychology as History

    Science.gov (United States)

    Gergen, Kenneth J.

    1973-01-01

    An analysis of theory and research in social psychology reveals that while methods of research are scientific in character, theories of social behavior are primarily reflections of contemporary history. (Author)

  4. Life History Approach

    DEFF Research Database (Denmark)

    Olesen, Henning Salling

    2015-01-01

    as in everyday life. Life histories represent lived lives past, present and anticipated future. As such they are interpretations of individuals’ experiences of the way in which societal dynamics take place in the individual body and mind, either by the individual him/herself or by another biographer. The Life...... History approach was developing from interpreting autobiographical and later certain other forms of language interactive material as moments of life history, i.e. it is basically a hermeneutic approach. Talking about a psycho-societal approach indicates the ambition of attacking the dichotomy...... of the social and the psychic, both in the interpretation procedure and in some main theoretical understandings of language, body and mind. My article will present the reflections on the use of life history based methodology in learning and education research as a kind of learning story of research work....

  5. History of Lake Andes

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Information about the history and management of Lake Andes is compiled in this report. It is intended to help future refuge managers become acquainted with the...

  6. History of Bioterrorism: Botulism

    Medline Plus

    Full Text Available ... Emergency Preparedness and You Video: "The History of Bioterrorism" Recommend on Facebook Tweet Share Compartir This video ... Note: Parts of this video were adapted from "Biological Warfare and Terrorism: The Military and Public Health ...

  7. Natural history of glaucoma

    National Research Council Canada - National Science Library

    Pan, Ying; Varma, Rohit

    2011-01-01

    .... This paper presents the natural history of open angle and angle closure glaucoma. We examine the glaucomatous progression in terms of changes in optic disk morphology and visual fields as well as the risk factors for progression...

  8. Teaching American Environmental History.

    Science.gov (United States)

    Strong, Douglas H.

    1974-01-01

    Themes, issues, teaching approaches, and bibliographic references to sources for a course in environmental history, offered with the hope of preparing students to formulate principles to guide our further conduct toward the environment, are described. (JH)

  9. Personal history, beyond narrative

    DEFF Research Database (Denmark)

    Køster, Allan

    2017-01-01

    Narrative theories currently dominate our understanding of how selfhood is constituted and concretely individuated throughout personal history. Despite this success, the narrative perspective has recently been exposed to a range of critiques. Whilst these critiques have been effective in pointing...... on a distinction between history and narrative, I outline an account of historical becoming through a process of sedimentation and a rich notion of what I call historical selfhood on an embodied level. Five embodied existentials are suggested, sketching a preliminary understanding of how selves are concretely...... out the shortcomings of narrative theories of selfhood, they have been less willing and able to suggest alternative ways of understanding personal history. In this article, I assess the criticisms and argue that an adequate phenomenology of personal history must also go beyond narrative. Drawing...

  10. Knowledge management and history

    OpenAIRE

    Ermine, Jean-Louis; Pauget, Bertrand; Beretti, Annie; Tortorici, Gilbert

    2004-01-01

    International audience; Capitalisation of the history of a technology, a technique or a concept within an industrial company is relevant to historians. However it largely exceeds the historical problems from a Knowledge Management point of view. In this context, it can be the subject of specific approaches especially Knowledge Engineering. However, it faces two types of difficulties: - The techniques in History have few modelling tools, and are even rather reticent with the use of such tools....

  11. History Vs. Expectations

    OpenAIRE

    Paul Krugman

    1989-01-01

    In models with external economies, there are often two or more long run equilibria. Which equilibrium is chosen? Much of the literature presumes that "history" sets initial conditions which determine the outcome, but an alternative view stresses the role of "expectations", i.e. of self-fulfilling prophecy. This paper uses a simple trade model with both external economies and adjustment costs to show how the parameters of the economy determine the relative importance of history and expectation...

  12. A tale of histories

    OpenAIRE

    Van Weert, Peter

    2008-01-01

    Constraint Handling Rules (CHR) is an elegant, high-level programming language based on multi-headed, forward chaining rules. A distinguishing feature of CHR are propagation rules. To avoid trivial non-termination, CHR implementations ensure a CHR rule is applied at most once with the same combination of constraints by maintaining a so-called propagation history. The performance impact of this history is often significant. We introduce two optimizations to reduce or even eliminate this overhe...

  13. Transformation of History textbooks

    DEFF Research Database (Denmark)

    Haue, Harry

    2013-01-01

    Artiklen omhandler danske og tyske lærebøger i historie over de seneste to århundreder med hensyn til deres vægtning af det nationale og det globale stof.......Artiklen omhandler danske og tyske lærebøger i historie over de seneste to århundreder med hensyn til deres vægtning af det nationale og det globale stof....

  14. Transformation of History textbooks

    DEFF Research Database (Denmark)

    Haue, Harry

    2013-01-01

    Artiklen omhandler danske og tyske lærebøger i historie over de seneste to århundreder med hensyn til deres vægtning af det nationale og det globale stof.......Artiklen omhandler danske og tyske lærebøger i historie over de seneste to århundreder med hensyn til deres vægtning af det nationale og det globale stof....

  15. Writing of Patient Case History

    Institute of Scientific and Technical Information of China (English)

    Tian Junying

    2016-01-01

    This paper introduces the language features, structure and contents of case history. Good patient case history contributes to correct diagnosis and formulation of a treatment plan, therefore, it is important for physicians to learn how to write case history.

  16. Demand and withdraw behaviors in couples with a history of infidelity.

    Science.gov (United States)

    Balderrama-Durbin, Christina M; Allen, Elizabeth S; Rhoades, Galena K

    2012-02-01

    Although relationship distress and dissolution are common consequences of sexual involvement outside a committed relationship, there is little empirical information regarding communication behaviors of couples who have experienced extradyadic involvement (EDI). This study examined male and female demand and withdraw behaviors in videotaped conflict discussions among 170 married or seriously dating couples categorized into 3 groups: those without a history of sexual EDI, those with a history of sexual EDI that was known to the other partner, and those with a history of "unknown" (undisclosed or undiscovered) sexual EDI. Both men and women in a relationship where there was at least one unknown EDI demonstrated the highest levels of demand behaviors. Furthermore, demand behaviors were higher for participating partners (those engaging in an outside sexual relationship) in relationships with an unknown EDI compared with participating partners in relationships with a known EDI. Conversely, demand behaviors were higher among nonparticipating partners in relationships with known EDI, compared to with nonparticipating partners in relationships with unknown EDI. Withdraw behaviors demonstrated a less pronounced and less consistent pattern of elevation by EDI group, and role within EDI, compared with demand behaviors. Clinical and relational implications for these findings are discussed.

  17. AAS Oral History Project

    Science.gov (United States)

    Buxner, Sanlyn; Holbrook, Jarita; AAS Oral History Team

    2016-06-01

    Now in its fourth year, the AAS Oral History Project has interviewed over 80 astronomers from all over the world. Led by the AAS Historical Astronomy Division (HAD) and partially funded by the American Institute of Physics Niels Bohr Library and ongoing support from the AAS, volunteers have collected oral histories from astronomers at professional meetings starting in 2015, including AAS, DPS, and the IAU general assembly. Each interview lasts one and a half to two hours and focuses on interviewees’ personal and professional lives. Questions include those about one’s family, childhood, strong influences on one’s scientific career, career path, successes and challenges, perspectives on how astronomy is changing as a field, and advice to the next generation. Each interview is audio recorded and transcribed, the content of which is checked with each interviewee. Once complete, interview transcripts are posted online as part of a larger oral history library at https://www.aip.org/history-programs/niels-bohr-library/oral-histories. Future analysis will reveal a rich story of astronomers and will help the community address issues of diversity, controversies, and the changing landscape of science. We are still recruiting individuals to be interviewed from all stages of career from undergraduate students to retired and emeritus astronomers. Contact Jarita Holbrook to schedule an interview or to find out more information about the project (astroholbrook@gmail.com). Also, contact Jarita Holbrook if you would like to become an interviewer for the project.

  18. To betray art history

    Directory of Open Access Journals (Sweden)

    Jae Emerling

    2016-12-01

    Full Text Available The work of Donald Preziosi represents one of the most sustained and often brilliant attempts to betray the modern discipline of art history by exposing its skillful shell game: precisely how and why it substitutes artifice, poetry, and representational schemes for putative facticity and objectivity (that desirous and yet ever elusive Kunstwissenschaft that art historians prattle on about. This attempt is inseparable from a sinuous, witty, involutive writing style that meanders between steely insight and coy suggestions of how art history could be performed otherwise. Preziosi’s writes art history. In doing so he betrays its disciplinary desires. It is this event of betrayal that has made his work so exciting to some, so troubling to others.

  19. History of psychiatry

    Science.gov (United States)

    Shorter, Edward

    2013-01-01

    Purpose of review The present review examines recent contributions to the evolving field of historical writing in psychiatry. Recent findings Interest in the history of psychiatry continues to grow, with an increasing emphasis on topics of current interest such as the history of psychopharmacology, electroconvulsive therapy, and the interplay between psychiatry and society. The scope of historical writing in psychiatry as of 2007 is as broad and varied as the discipline itself. Summary More than in other medical specialties such as cardiology or nephrology, treatment and diagnosis in psychiatry are affected by trends in the surrounding culture and society. Studying the history of the discipline provides insights into possible alternatives to the current crop of patent-protected remedies and trend-driven diagnoses. PMID:18852567

  20. User Interface History

    DEFF Research Database (Denmark)

    Jørgensen, Anker Helms; Myers, Brad A

    2008-01-01

    User Interfaces have been around as long as computers have existed, even well before the field of Human-Computer Interaction was established. Over the years, some papers on the history of Human-Computer Interaction and User Interfaces have appeared, primarily focusing on the graphical interface era...... and early visionaries such as Bush, Engelbart and Kay. With the User Interface being a decisive factor in the proliferation of computers in society and since it has become a cultural phenomenon, it is time to paint a more comprehensive picture of its history. This SIG will investigate the possibilities...... of  launching a concerted effort towards creating a History of User Interfaces. ...

  1. Tranquebar - Whose History?

    DEFF Research Database (Denmark)

    Jørgensen, Helle

    This thesis comprises an ethnography of the contemporary practices through which the town of Tranquebar emerges as a heritage town with a point of departure in a particular part of its colonial history. Formally declared as heritage by the government of Tamil Nadu due to its well-preserved townsc......This thesis comprises an ethnography of the contemporary practices through which the town of Tranquebar emerges as a heritage town with a point of departure in a particular part of its colonial history. Formally declared as heritage by the government of Tamil Nadu due to its well...

  2. Time, money, and history.

    Science.gov (United States)

    Edgerton, David

    2012-06-01

    This essay argues that taking the economy seriously in histories of science could not only extend the range of activities studied but also change--often quite radically--our understanding of well-known cases and instances in twentieth-century science. It shows how scientific intellectuals and historians of science have followed the money as a means of critique of particular forms of science and of particular conceptions of science. It suggests the need to go further, to a much broader implicit definition of what constitutes science--one that implies a criticism of much history of twentieth-century science for defining it implicitly and inappropriately in very restrictive ways.

  3. History of psychology.

    Science.gov (United States)

    Weidman, Nadine

    2016-02-01

    The editor of History of Psychology discusses her plan to vary the journal's content and expand its scope in specific ways. The first is to introduce a "Spotlight" feature, a relatively brief, provocative thought piece that might take one of several forms. Along with this new feature, she hopes further to broaden the journal's coverage and its range of contributors. She encourages submissions on the history of the psy-sciences off the beaten path. Finally, she plans to continue the journal's tradition of special issues, special sections, and essay reviews of two or more important recently published books in the field. (PsycINFO Database Record

  4. History, Memory and Film

    DEFF Research Database (Denmark)

    Bondebjerg, Ib

    In this paper I discuss history and memory from a theoretical and philosophical point of view and the non-fiction and fiction aspects of historical representation. I use Edgar Reitz’ monumental work Heimat 1-3 (and his recent film Die Andere Heimat) as examples of very different transformative...... historical narratives. In terms of narrative construction and aesthetic form the Heimat-project challenges the dominant forms of historical fiction. By combining personal memory, everyday life and collective memory and a more indirect way of representing factual history Reitz wants to transform our look...

  5. The hidden holes in history.

    Science.gov (United States)

    Riegelman, R K

    1981-09-01

    History taking can be viewed as a diagnostic test. Identification of the purpose, accuracy, reproducibility, and diagnostic discrimination of the history assists in reliable history taking. An appreciation of the strengths and limitations of history taking helps physicians effectively utilize this important diagnostic tool.

  6. A History of Multimedia.

    Science.gov (United States)

    Burke, Larry Kenneth

    The history of multimedia and descriptions of various multimedia events from 1900 to 1972 are presented. The development of multimedia events is described for four eras and four main classifications of events: multiscreen presentations, electronic media and performers, environmental theater, and environments. Five appendixes include a discussion…

  7. Writing History in Exile

    NARCIS (Netherlands)

    de Baets, Antoon; Berger, Stefan

    2016-01-01

    WRITING HISTORY IN EXILE * Stefan Berger and Antoon De Baets, Reflections on Exile Historiography 11 * Antoon De Baets, Plutarch’s Thesis : the Contribution of Refugee Historians to Historical Writing (1945-2015) 27 * Peter Burke, Silver Lining : on Some Intellectual Benefits of Exile 39 * Ragnar

  8. Freud and cultural history.

    Science.gov (United States)

    Burke, Peter

    2007-01-01

    This article is concerned with three questions: Can historians make use of Freud's ideas? Have they done so in the past? Should they do so in the future? It begins with a personal encounter with Freud's ideas and then raises some general questions about the relation between psychoanalysis and history.

  9. Bengal Literature and History.

    Science.gov (United States)

    Dimock, Edward C., Jr., Ed.

    The unifying theme of the papers in this book is the use of creative literature as source material for the study of cultural history. Titles and authors of the papers are: "Encounter and Growth in Bengali Literature, A Survey of Medieval Bengali Literature" by T.W. Clark; "The Hindu Chiefdom in Middle Bengali Literature" by…

  10. My English History.

    Science.gov (United States)

    Sommer, Paul

    2003-01-01

    Outlines the author's history as an English teacher. Suggests that English teaching is not hermetic but profoundly influenced by educational trends outside the subject itself. Explains that the author's commitment to English combines a sense of engagement with a sense of liberation. (PM)

  11. Epistemology and history.

    Science.gov (United States)

    de Chadarevian, Soraya

    2013-01-01

    The essay reviews Hans-Jörg Rheinberger's intensive engagement with French epistemology that has remained a constant throughout his career. It focuses especially on a set of recent writings by Rheinberger that all revolve around the question: how has epistemology become historical? This question is discussed in the context of Rheinberger's own move from philosophy to an embracement of history.

  12. Access and History

    DEFF Research Database (Denmark)

    Jensen, Erik Granly

    2012-01-01

    Like many other major European media corporations the Danish Broadcasting Corporation (DR) has begun the digitisation of its audio-visual archives. This transformation from analogue to digital archives raises a number of questions regarding the archive specific character, history and content...

  13. [Brief history of dreams].

    Science.gov (United States)

    Rosselli, D

    Throughout history dreams have played a crucial role. Dreams have inspired great works of art, solved scientific problems and, because of the premonitory value attached to them, have influenced transcendental decisions. This paper reviews some of the dreams that have been a part of the world's literature and historical tradition.

  14. History in the Flesh

    DEFF Research Database (Denmark)

    Bencard, Adam

    drevet af en historisering, en vilje til at placere historie hvor der før var biologi.   Denne afhandling undersøger denne interesse i kroppen gennem en analyse af hvad jeg kalder den historiserede krop som diskursiv figur. Den historiserede krop er ikke et klart aftegnet koncept eller en skarp afgrænset...

  15. CERN's Early History Revisited

    CERN Multimedia

    Schopper, Herwig Franz; Krige, Gerhard John

    2005-01-01

    As a member of the group of historians charged to write the history of the founding of CERN, John Krige particularly underlines the important role I.I. Rabi played. The first author, former Director General of CERN add a few comments. S.A. Khan gives precisions about the role played by E. Amaldi and P. Auger; then J. Krige replies

  16. American History (an introduction)

    DEFF Research Database (Denmark)

    Nye, David Edwin

    I et letforståeligt engelsk giver professor David Nye en fængende præsentation af amerikansk historie fra den tidlige kolonisationsperiode til præsident Obama. Bogen giver et helhedsportræt af perioderne og inkluderer til hver periode en kortfattet præsentation af kultur- og litteraturhistorien....

  17. Atmospheric refraction : a history

    NARCIS (Netherlands)

    Lehn, WH; van der Werf, S

    2005-01-01

    We trace the history of atmospheric refraction from the ancient Greeks up to the time of Kepler. The concept that the atmosphere could refract light entered Western science in the second century B.C. Ptolemy, 300 years later, produced the first clearly defined atmospheric model, containing air of un

  18. Psychology and History.

    Science.gov (United States)

    Munsterburg, Hugo

    1994-01-01

    This essay considers the discipline of psychology as distinct from history, defining it as a science within philosophy dedicated to the study of the causal structure of the human mind. Although Hugo Munsterburg was considered an important figure in applied psychology, this essay represents an earlier epistemology. (SLD)

  19. American History (an introduction)

    DEFF Research Database (Denmark)

    Nye, David Edwin

    I et letforståeligt engelsk giver professor David Nye en fængende præsentation af amerikansk historie fra den tidlige kolonisationsperiode til præsident Obama. Bogen giver et helhedsportræt af perioderne og inkluderer til hver periode en kortfattet præsentation af kultur- og litteraturhistorien....

  20. Diving Into History

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Three divers have made history at the 13th FINA World Championships held in Rome on July 17-August 2. Guo Jingjing, the Chinese diving queen who clinched the gold in the women’s 3-meter springboard on July 21, became the first

  1. History, Theory, and Education

    Science.gov (United States)

    Rury, John L.

    2011-01-01

    In this article, the author discusses the question of theory as it may pertain to the history of education, with particular attention to the United States. Historians, like everyone else, have little choice regarding the use of theory; to one extent or another they must. The question is how much and to what end. The author aims to consider the…

  2. Taarnborg: Becoming History

    DEFF Research Database (Denmark)

    Marchetti, Emanuela; Dai, Zheng; Omarsson, Olafur

    Focus of this project is an inquiry in the materialisation of the historical heritage of Taarnborg (Ribe, Denmark) once a place related to local religious authorities, now a private home and museum, where visitors attend to musical events and lectures about history and theology. Following...

  3. Didactics of History

    DEFF Research Database (Denmark)

    Haue, Harry

    The book consits of five chapters about  formation and education in Denmark over the last two centuries. The developement of history teaching is especially stressed. The guiding concept for the upper secondary education has since 1850 been 'general character formation'. The book is an edited...

  4. Writing History in Exile

    NARCIS (Netherlands)

    de Baets, Antoon; Berger, Stefan

    2016-01-01

    WRITING HISTORY IN EXILE * Stefan Berger and Antoon De Baets, Reflections on Exile Historiography 11 * Antoon De Baets, Plutarch’s Thesis : the Contribution of Refugee Historians to Historical Writing (1945-2015) 27 * Peter Burke, Silver Lining : on Some Intellectual Benefits of Exile 39 * Ragnar Bj

  5. Runaways in History

    Science.gov (United States)

    Lipschutz, Mark R.

    1977-01-01

    Although running away is a glorified part of American folk tradition, today's runaway runs afoul of a host of laws, including those which make the act itself illegal. This article examines the history of the social and legal status of youth and why they run away. (Author)

  6. Teaching Local History.

    Science.gov (United States)

    Singer, Alan, Ed.

    2003-01-01

    This Social Science Docket theme issue focuses on teaching local history and included theme and non-themed articles, lesson plans, learning activities, and book, movie, and museum reviews designed for K-12 social studies teachers. Articles and materials in this issue are: "Editing Is Not Censorship" (Alan Singer); "Teachers Respond…

  7. History of vaccination.

    Science.gov (United States)

    Plotkin, Stanley

    2014-08-26

    Vaccines have a history that started late in the 18th century. From the late 19th century, vaccines could be developed in the laboratory. However, in the 20th century, it became possible to develop vaccines based on immunologic markers. In the 21st century, molecular biology permits vaccine development that was not possible before.

  8. Narrative History and Theory

    Science.gov (United States)

    Tamura, Eileen H.

    2011-01-01

    While narrative history has been the prevailing mode in historical scholarship, its preeminence has not gone unquestioned. In the 1980s, the role of narrative in historical writing was "the subject of extraordinarily intense debate." The historical backdrop of this debate can be traced to the preceding two decades, when four groups of thinkers…

  9. Didactics of History

    DEFF Research Database (Denmark)

    Haue, Harry

    The book consits of five chapters about  formation and education in Denmark over the last two centuries. The developement of history teaching is especially stressed. The guiding concept for the upper secondary education has since 1850 been 'general character formation'. The book is an edited...

  10. Ownership and Object History

    Science.gov (United States)

    Friedman, Ori; Neary, Karen R.; Defeyter, Margaret A.; Malcolm, Sarah L.

    2011-01-01

    Appropriate behavior in relation to an object often requires judging whether it is owned and, if so, by whom. The authors propose accounts of how people make these judgments. Our central claim is that both judgments often involve making inferences about object history. In judging whether objects are owned, people may assume that artifacts (e.g.,…

  11. Walking Out of History

    Institute of Scientific and Technical Information of China (English)

    GARMARDORJE; CUIFENG

    2002-01-01

    EDITOR’S NOTE: Barkor Street around Jokhang Monastery has a history of 1,300 years. The age-old street, however, has found the fountain of youth and takes on a new outlook today. And the fountain of youth is none other than the ongoing reform and opening program.

  12. In a Word, History

    Science.gov (United States)

    Dohan, Mary Helen

    1977-01-01

    Understanding words like "bionics" will open the mind to the horizons of another time when words like "railroad" evoked wonder and "to fly to the moon" was a metaphor for the impossible dream. Suggests that history teachers and English teachers should join together in using words to teach both subjects. (Editor/RK)

  13. Big data in history

    CERN Document Server

    Manning, Patrick

    2013-01-01

    Big Data in History introduces the project to create a world-historical archive, tracing the last four centuries of historical dynamics and change. Chapters address the archive's overall plan, how to interpret the past through a global archive, the missions of gathering records, linking local data into global patterns, and exploring the results.

  14. Natural history of COPD

    DEFF Research Database (Denmark)

    Vestbo, Jørgen; Lange, Peter

    2016-01-01

    The natural history of chronic obstructive pulmonary disease (COPD) is usually described with a focus on change in forced expiratory volume in 1 s (FEV1 ) over time as this allows for exploration of risk factors for an accelerated decline-and thus of developing COPD. From epidemiological studies we...

  15. Atmospheric refraction : a history

    NARCIS (Netherlands)

    Lehn, WH; van der Werf, S

    2005-01-01

    We trace the history of atmospheric refraction from the ancient Greeks up to the time of Kepler. The concept that the atmosphere could refract light entered Western science in the second century B.C. Ptolemy, 300 years later, produced the first clearly defined atmospheric model, containing air of

  16. Figuring Out Art History

    CERN Document Server

    Schich, Maximilian

    2015-01-01

    World population and the number of cultural artifacts are growing exponentially or faster, while cultural interaction approaches the fidelity of a global nervous system. Every day hundreds of millions of images are loaded into social networks by users all over the world. As this myriad of new artifacts veils the view into the past, like city lights covering the night sky, it is easy to forget that there is more than one Starry Night, the painting by Van Gogh. Like in ecology, where saving rare species may help us in treating disease, art and architectural history can reveal insights into the past, which may hold keys to our own future. With humanism under threat, facing the challenge of understanding the structure and dynamics of art and culture, both qualitatively and quantitatively, is more crucial now than it ever was. The purpose of this article is to provide perspective in the aim of figuring out the process of art history - not art history as a discipline, but the actual history of all made things, in t...

  17. Testing History As Inquiry

    Science.gov (United States)

    Miller, James R.; Hart, James

    1973-01-01

    Some obvious difficulties of teaching and testing history as inquiry are reviewed. Examples of test items that require students to utilize thought processes developed through inquiry teaching are presented together with a rationale for their use. Bloom's Taxonomy of Educational Objectives is proposed as a useful tool in test construction. (SM)

  18. BRST Quantisation of Histories Electrodynamics

    OpenAIRE

    Noltingk, D.

    2001-01-01

    This paper is a continuation of earlier work where a classical history theory of pure electrodynamics was developed in which the the history fields have \\emph{five} components. The extra component is associated with an extra constraint, thus enlarging the gauge group of histories electrodynamics. In this paper we quantise the classical theory developed previously by two methods. Firstly we quantise the reduced classical history space, to obtain a reduced quantum history theory. Secondly we qu...

  19. STATISTICAL METHODS IN HISTORY

    Directory of Open Access Journals (Sweden)

    Orlov A. I.

    2016-01-01

    Full Text Available We have given a critical analysis of statistical models and methods for processing text information in historical records to establish the times when there were certain events, ie, to build science-based chronology. There are three main kinds of sources of knowledge of ancient history: ancient texts, the remains of material culture and traditions. The specific date of the extracted by archaeologists objects in most cases can not be found. The group of Academician A.T. Fomenko has developed and applied new statistical methods for analysis of historical texts (Chronicle, based on the intensive use of computer technology. Two major scientific results were: the majority of historical records that we know now, are duplicated (in particular, chronicles, describing the so-called "Ancient Rome" and "Middle Ages", talking about the same events; the known historical chronicles tell us about real events, separated from the present time for not more than 1000 years. It was found that chronicles describing the history of "ancient times" and "Middle Ages" and the chronicle of Chinese history and the history of various European countries do not talk about different, but about the same events. We have the attempt of a new dating of historical events and restoring the true history of human society based on new data. From the standpoint of statistical methods of historical records and images of their fragments – they are special cases of non-numeric objects of nature. Therefore, developed by the group of A.T. Fomenko computer-statistical methods are the part of non-numerical statistics. We have considered some methods of statistical analysis of chronicles applied by the group of A.T. Fomenko: correlation method of maximums; dynasties method; the method of attenuation frequency; questionnaire method codes. New chronology allows us to understand much of the battle of ideas in modern science and mass consciousness. It becomes clear the root cause of cautious

  20. Report on EUROMET.RI(I)-K1 and EUROMET.RI(I)-K4 (EUROMET project no. 813): Comparison of air kerma and absorbed dose to water measurements of {sup 60}Co radiation beams for radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Csete, I. [National Office of Measures (OMH) - pilot laboratory and corresponding author (Hungary); Leiton, A.G. [Research Centre for Energy, Environment and Technology (CMRI-CIEMAT) (Spain); Sochor, V. [Czech Metrology Institute (CMI) (Czech Republic); Lapenas, A. [Latvian National Metrology Center (LNMC-RMTC) (Latvia); Grindborg, J.E. [Swedish Radiation Protection Authority (SSI) (Sweden); Jokelainen, I. [Radiation and Nuclear Safety Authority (STUK) (Finland); Bjerke, H. [Norwegian Radiation Protection Authority (NRPA) (Norway); Dobrovodsky, J. [Slovak Institute of Metrology (SMU) (Slovakia); Megzifene, A. [International Atomic Energy Agency, IAEA, Vienna (Austria); Hourdakis, C.J. [Hellenic Atomic Energy Committee (HAEC-HIRCL) (Greece); Ivanov, R. [National Centre of Metrology (NCM) (Bulgaria); Vekic, B. [Rudjer Boskovic Institute (IRB) (Croatia); Kokocinski, J. [Central Office of Measures (GUM) (Poland); Cardoso, J. [Institute for Nuclear Technology (ITN-LMRIR) (Portugal); Buermann, L. [Physikalisch Technische Bundesanstalt (PTB) (Germany); Tiefenboeck, W. [Bundesamt fur Eich und Vermesungswesen (BEV) (Austria); Stucki, G. [17 Bundesamt fur Metrologie (METAS) (Switzerland); Van Dijk, E. [NMi Van Swinden Laboratorium (NMi) (Netherlands); Toni, M.P. [ENEA-CR Istituto Nazionale di Metrologia delle Radiazioni Ionizzanti (ENEA) (Italy); Minniti, R. [20 National Institute of Standards and Technology (NIST) (United States); McCaffrey, J.P. [National Research Council Canada (NRC) (Canada); Silva, C.N.M. [National Metrology Laboratory of Ionizing Radiation (LNMRI-IRD) (Brazil); Kharitonov, I. [D I Mendeleyev Institute for Metrology (VNIIM) (RU); Webb, D. [Australian Radiation Protection and Nuclear Safety Agency (ARPANSA) (Australia); Saravi, M. [National Atomic Energy Commission (CNEA-CAE) (Argentina); Delaunay, F. [Laboratoire National Henri Becquerel (LNE-LNHB) (France)

    2010-06-15

    The results of an unprecedented international effort involving 26 countries are reported. The EUROMET.RI(I)-K1 and EUROMET.RI(I)-K4 key comparisons were conducted with the goal of supporting the relevant calibration and measurement capabilities (CMC) planned for publication by the participant laboratories. The measured quantities were the air kerma (K{sub air}) and the absorbed dose to water (Dw) in {sup 60}Co radiotherapy beams. The comparison was conducted by the pilot laboratory MKEH (Hungary), in a star-shaped arrangement from January 2005 to December 2008. The calibration coefficients of four transfer ionization chambers were measured using two electrometers. The largest deviation between any two calibration coefficients for the four chambers in terms of air kerma and absorbed dose to water was 2.7% and 3.3% respectively. An analysis of the participant uncertainty budgets enabled the calculation of degrees of equivalence (DoE), in terms of the deviations of the results and their associated uncertainties. As a result of this EUROMET project 813 comparison, the BIPM key comparison database (KCDB) will include eleven new Kair and fourteen new D{sub w} DoE values of European secondary standard dosimetry laboratories (SSDLs), and the KCDB will be updated with the new DoE values of the other participant laboratories. The pair-wise degrees of equivalence of participants were also calculated. In addition to assessing calibration techniques and uncertainty calculations of the participants, these comparisons enabled the experimental determinations of N{sub Dw}/N{sub Kair} ratios in the {sup 60}Co gamma radiation beam for the four radiotherapy transfer chambers. (authors)

  1. THE VOLATILE COMPOSITION OF COMET C/2003 K4 (LINEAR) AT NEAR-IR WAVELENGTHS—COMPARISONS WITH RESULTS FROM THE NANÇAY RADIO TELESCOPE AND FROM THE ODIN, SPITZER, AND SOHO SPACE OBSERVATORIES

    Energy Technology Data Exchange (ETDEWEB)

    Paganini, L.; Mumma, M. J.; Villanueva, G. L.; DiSanti, M. A.; Bonev, B. P., E-mail: lucas.paganini@nasa.gov [Goddard Center for Astrobiology, NASA GSFC, MS 690, Greenbelt, MD 20771 (United States)

    2015-07-20

    We observed comet C/2003 K4 (LINEAR) using NIRSPEC at the Keck Observatory on UT 2004 November 28, when the comet was at 1.28 AU from the Sun (post-perihelion) and 1.38 AU from Earth. We detected six gaseous species (H{sub 2}O, OH*, C{sub 2}H{sub 6}, CH{sub 3}OH, CH{sub 4}, and HCN) and obtained upper limits for three others (H{sub 2}CO, C{sub 2}H{sub 2}, and NH{sub 3}). Our results indicate a water production rate of (1.72 ± 0.18) × 10{sup 29} molecules s{sup −1}, in reasonable agreement with production rates from SOHO (on the same day), Odin (one day earlier), and Nançay (about two weeks earlier). We also report abundances (relative to water) for seven trace species: CH{sub 3}OH (∼1.8%), CH{sub 4} (∼0.9%), and C{sub 2}H{sub 6} (∼0.4%) that were consistent with mean values among Oort cloud (OC) comets, while NH{sub 3} (<0.55%), HCN (∼0.07%), H{sub 2}CO (<0.07%), and C{sub 2}H{sub 2} (<0.04%) were “lower” than the mean values in other OC comets. We extracted inner-coma rotational temperatures for four species (H{sub 2}O, C{sub 2}H{sub 6}, CH{sub 3}OH, and CH{sub 4}), all of which are consistent with 70 K (within 1σ). The extracted ortho-para ratio for water was 3.0 ± 0.15, corresponding to spin temperatures larger than 39 K (at the 1σ level) and agreeing with those obtained with the Spitzer Space Telescope at the 2σ level.

  2. Micro–Positron Emission Tomography/Contrast-Enhanced Computed Tomography Imaging of Orthotopic Pancreatic Tumor–Bearing Mice Using the αvβ3 Integrin Tracer 64Cu-Labeled Cyclam-RAFT-c(-RGDfK-4

    Directory of Open Access Journals (Sweden)

    Winn Aung

    2013-09-01

    Full Text Available The purpose of this study was to develop a clinically relevant orthotopic xenotransplantation model of pancreatic cancer and to perform a preclinical evaluation of a new positron emission tomography (PET imaging probe, 64Cu-labeled cyclam-RAFT-c(-RGDfK-4 peptide (64Cu-RAFT-RGD, using this model. Varying degrees of αvβ3 integrin expression in several human pancreatic cancer cell lines were examined by flow cytometry and Western blotting. The cell line BxPC-3, which is stably transfected with a red fluorescence protein (RFP, was used for surgical orthotopic implantation. Orthotopic xenograft was established in the pancreas of recipient nude mice. An in vivo probe biodistribution and receptor blocking study, preclinical PET imaging coregistered with contrast-enhanced computed tomography (CECT comparing 64Cu-RAFT-RGD and 18F-fluoro-2-deoxy-D-glucose (18F-FDG accumulation in tumor, postimaging autoradiography, and histologic and immunohistochemical examinations were done. Biodistribution evaluation with a blocking study confirmed that efficient binding of probe to tumor is highly αvβ3 integrin specific. 64Cu-RAFT-RGD PET combined with CECT provided for precise and easy detection of cancer lesions. Autoradiography, histologic, and immunohistochemical examinations confirmed the accumulation of 64Cu-RAFT-RGD in tumor versus nontumor tissues. In comparative PET studies, 64Cu-RAFT-RGD accumulation provided better tumor contrast to background than 18F-FDG. Our results suggest that 64Cu-RAFT-RGD PET imaging is potentially applicable for the diagnosis of αvβ3 integrin–expressing pancreatic tumors.

  3. Living in history

    DEFF Research Database (Denmark)

    Brown, Norman R.; Lee, Peter J.; Krslak, Mirna

    2009-01-01

    Foreslår et paradigme - "Living in history" - til undersøgelse af forholdet mellem samtidshistorie og selvbiografisk hukommelse. Metoden spørger ikke direkte og altså ikke til den anknytning, man bevidst ville vælge at fremhæve, men undersøger indirekte, om der spontant associeres til samtidsbegi......Foreslår et paradigme - "Living in history" - til undersøgelse af forholdet mellem samtidshistorie og selvbiografisk hukommelse. Metoden spørger ikke direkte og altså ikke til den anknytning, man bevidst ville vælge at fremhæve, men undersøger indirekte, om der spontant associeres til...

  4. Tranquebar - Whose History?

    DEFF Research Database (Denmark)

    Jørgensen, Helle

    in which the townscape of Tranquebar has become subject to promotion as amaterial embodiment of transnational history, and thereby to explore the negotiations of historicity that come into play amongst the many stakeholders in this process, including residents, heritage and tourism developers, public......This thesis comprises an ethnography of the contemporary practices through which the town of Tranquebar emerges as a heritage town with a point of departure in a particular part of its colonial history. Formally declared as heritage by the government of Tamil Nadu due to its well......-preserved townscape from the Indo-Danish colonial period in 1620-1845, the town has for decades been subject to both Danish and Indian attempts at preserving and promoting it as a destination of heritage tourism. In this process claims are made on the town as an expression of cross-cultural heritage; but whose...

  5. What history tells us

    Indian Academy of Sciences (India)

    Michel Morange

    2005-06-01

    The history of science was long considered to be something peripheral to science itself. By supplying interesting stories and gossip, it seemed, at best, to provide material for enlivening lectures. In general, it was deemed a suitable activity for retired scientists. This view has been revised considerably in the past years and indeed, today seems hopelessly out of date. History and philosophy of science are increasingly held to be an essential component of the education of scientists. By becoming acquainted with these areas, practicing scientists – and in particular biologists – can better appreciate the significance of the models and theories that underpin their research, especially with the accelerating succession of one idea by the next. The present series, of which the article that follows is the first, aims to give historical glimpses that bear on contemporary biology. The hope is that these glimpses will be both a source of inspiration and of help in resisting useless fashions.

  6. My brief history

    CERN Document Server

    Hawking, Stephen

    2013-01-01

    My Brief History recounts Stephen Hawking’s improbable journey, from his postwar London boyhood to his years of international acclaim and celebrity. Lavishly illustrated with rarely seen photographs, this concise, witty, and candid account introduces readers to a Hawking rarely glimpsed in previous books: the inquisitive schoolboy whose classmates nicknamed him Einstein; the jokester who once placed a bet with a colleague over the existence of a particular black hole; and the young husband and father struggling to gain a foothold in the world of physics and cosmology. Writing with characteristic humility and humor, Hawking opens up about the challenges that confronted him following his diagnosis of ALS at age twenty-one. Tracing his development as a thinker, he explains how the prospect of an early death urged him onward through numerous intellectual breakthroughs, and talks about the genesis of his masterpiece A Brief History of Time—one of the iconic books of the twentieth century.

  7. [Arthritis and clinical history].

    Science.gov (United States)

    Silva, Lígia; Sampaio, Luzia; Pinto, José; Ventura, Francisco S

    2011-01-01

    In front of a patient with arthritis, clinical good-sense tells that the most probable diagnosis are the most prevalent ones. Nevertheless, we have to exclude a multiplicity of other aetiologies, less frequent, but with highest implications in the therapeutic conduct. Infections by Brucella and by Borrelia are rare causes of chronic arthritis, yet are diagnosis to consider, even when the clinical manifestations aren't the most typical, as there still exist endemic areas in Portugal. Here we report two clinical cases about patients with arthritis for more than one year, subject to ineffective exams ant treatments. Only the clinical history could put on evidence clinical-epidemiological data, suggestive of Brucellosis and Lyme Disease, namely the professional contact with infected animals, and the history of probable erythema migrans, that pointed toward the correct diagnosis. So, with directed therapeutic, there was complete resolution of the inflammatory symptoms.

  8. History of Korean Neurosurgery.

    Science.gov (United States)

    Hwang, Sung-nam

    2015-08-01

    The year 2012 was the 50th anniversary of the Korean Neurosurgical Society, and in 2013, the 15th World Congress of Neurosurgery took place in Seoul, Korea. Thus, it is an appropriate occasion to introduce the world to the history of the Korean Neurosurgical Society and the foundation, development, and growth of Korean neurosurgery. Historical materials and pictures were collected and reviewed from the history book and photo albums of the Korean Neurosurgical Society. During the last 50 years, the Korean Neurosurgical Society and Korean neurosurgery have developed and grown enormously not only in quantity but also in quality. In every aspect, the turning point from the old to the new era of the Korean Neurosurgical Society and Korean neurosurgery was the year 1980. Copyright © 2015. Published by Elsevier Inc.

  9. Weather Fundamentals: Hurricanes & Tornadoes. [Videotape].

    Science.gov (United States)

    1998

    The videos in this educational series, for grades 4-7, help students understand the science behind weather phenomena through dramatic live-action footage, vivid animated graphics, detailed weather maps, and hands-on experiments. This episode (23 minutes) features information on the deadliest and most destructive storms on Earth. Through satellite…

  10. Bug City: Ladybugs & Fireflies [Videotape].

    Science.gov (United States)

    1998

    "Bug City" is a video series created to help children (grades 1-6) learn about insects and other small critters. All aspects of bug life are touched upon, including body structure, food, habitat, life cycle, mating habits, camouflage, mutualism (symbiosis), adaptations, social behavior, and more. Each program features dramatic…

  11. Bug City: Flies & Mosquitoes [Videotape].

    Science.gov (United States)

    1998

    "Bug City" is a video series created to help children (grades 1-6) learn about insects and other small critters. All aspects of bug life are touched upon, including body structure, food, habitat, life cycle, mating habits, camouflage, mutualism (symbiosis), adaptations, social behavior, and more. Each program features dramatic microscopic…

  12. Bug City: Ladybugs & Fireflies [Videotape].

    Science.gov (United States)

    1998

    "Bug City" is a video series created to help children (grades 1-6) learn about insects and other small critters. All aspects of bug life are touched upon, including body structure, food, habitat, life cycle, mating habits, camouflage, mutualism (symbiosis), adaptations, social behavior, and more. Each program features dramatic…

  13. History of insulin

    Directory of Open Access Journals (Sweden)

    Celeste C. Quianzon

    2012-07-01

    Full Text Available The advancement of diabetes treatment has gone from crude extracts of insulin and accidental discovery of sulfa-like drugs in antibiotics to the development of drugs based on improved understanding of the pathophysiology of diabetes mellitus. This article will review the history of the discovery and development of insulin. A companion focusing on non-insulin diabetes agents will follow in the next issue of JCHIMP.

  14. Wireless radio a history

    CERN Document Server

    Coe, Lewis

    2006-01-01

    ""Informative...recommended""--Choice; ""interesting...a good read...well worth reading""--Contact Magazine. This history first looks at Marconi's wireless communications system and then explores its many applications, including marine radio, cellular telephones, police and military uses, television and radar. Radio collecting is also discussed, and brief biographies are provided for the major figures in the development and use of the wireless.

  15. History of hadrontherapy

    Science.gov (United States)

    Amaldi, Ugo

    2015-05-01

    Hadrontherapy is today an established modality in cancer radiation therapy. Based on the superior ballistic and radiobiological properties of accelerated ions, this discipline experienced a remarkable growth in the last 20 years. This paper reviews the history of hadrontherapy, from the early days to the most recent developments. In particular, the evolution of proton and carbon ion therapy is presented together with a glance at future solutions such as single-room facilities.

  16. The Trouble with History

    Science.gov (United States)

    2005-01-01

    regards, the post-structuralist theories of Michel Foucault, the deconstruc- tionist notions of Jacques Derrida , and Dominick LaCapra’s techniques of...is closer to fiction than nonfiction. Indeed, they have a point. Compare: Frank Ankersmit, “Historiography and Postmodernism ,” History and Theory, 28...Historiography in the Twentieth Century: From Scientific Objectivity to the Postmodern Challenge (Hanover, N.H.: Wesleyan Univ. Press, 1997) and Evans

  17. A quantum of history

    CERN Document Server

    Holland, Peter

    2014-01-01

    With reference to primary sources it is shown that key claims made regarding the history of the pilot wave theory in Quantum Theory at the Crossroads are not supported by the historical record. It is also argued that the association of de Broglie with just a first-order law of particle motion, and Bohm with a second-order one, has no historical basis.

  18. Troubling Histories and Theories: Gender and the History of Education.

    Science.gov (United States)

    Goodman, Joyce

    2003-01-01

    Discusses gender influences and ways that history and theory have interacted in influencing women's contribution and recognition in educational history. Focuses on several historians' views and how some have eventually written women back into the historical picture of education. (KDR)

  19. History magazines in the UK

    OpenAIRE

    Haydn, Terry

    2013-01-01

    The paper explores the phenomenon of popular history magazines as a facet of public history. The UK has seen a substantial increase in the number of popular history magazines available to the public, with some magazines reaching high levels of circulation. The paper looks at the range of magazines available – from ‘heritage’ and ‘family’ history, to special interest magazines, and more ‘serious’ and scholarly history magazines. What is it that makes history magazines sell, and what influence ...

  20. Cultural History and its Neighbours

    OpenAIRE

    Burke, Peter

    2012-01-01

    This article offers a kind of map of intellectual fields that are more or less close to cultural history. It is difficult to separate cultural history from intellectual, social and political history, from archaeology and from the histories of such activities as art, literature, language and religion, whether these histories are studied in departments of history or under the umbrella of “visual studies”, “religious studies” or “cultural studies”. All these neighbours form an “inner circle”, di...

  1. Teacher Candidates' Attitudes to Using Oral History in History Education

    Science.gov (United States)

    Demircioglu, Ebru

    2016-01-01

    The aim of this research is to determine the views of history teacher candidates towards an oral history project carried out in the Special Teaching Method Course of the history pedagogy program of the Fatih Faculty of Education (FFE) at Karadeniz Technical University in Turkey. An open-ended questionnaire and semi-structured interview were the…

  2. The History of Secondary Education in History of Education

    Science.gov (United States)

    McCulloch, Gary

    2012-01-01

    "History of Education" has published a steady stream of papers on the history of secondary education over the first 40 years of its existence. This corpus of research has been generated in the context of renewed interest in the history of secondary education that has been stimulated by developments in social and historical inquiry as…

  3. Disciplinary History and the Situation of History Teachers

    Science.gov (United States)

    Fordham, Michael

    2012-01-01

    A growing consensus in the history education community supports the idea that the discipline of history provides the best opportunity for moving beyond a "nation-building" or "skills-based" approach to teaching about the past. The social and temporal characteristics of the discipline of history mean such conclusions have…

  4. Enhanced Master Station History Report

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Enhanced Master Station History Report (EMSHR) is a compiled list of basic, historical information for every station in the station history database, beginning...

  5. Quantum histories without contrary inferences

    Science.gov (United States)

    Losada, Marcelo; Laura, Roberto

    2014-12-01

    In the consistent histories formulation of quantum theory it was shown that it is possible to retrodict contrary properties. We show that this problem do not appear in our formalism of generalized contexts for quantum histories.

  6. Summary of The History Manifesto.

    Science.gov (United States)

    Jacobs, Noortje

    2016-06-01

    This essay provides a brief, impartial summary of some main points of The History Manifesto, of the debate among historians that it has engendered, and of its connection to previous debates in and about the history of the sciences.

  7. Inventing the History of Invention.

    Science.gov (United States)

    Molella, Arthur P.

    1988-01-01

    Selected are three pioneers who invented the history of technology: Abbott P. Usher, Lewis Mumford, and Sigfried Giedion. Their careers, publications, and contributions to the history of technology are described. (YP)

  8. Integrating Educational Theory and History

    Science.gov (United States)

    Johnson, James P.

    1977-01-01

    Recommends that history teachers incorporate educational theory into their courses. Describes and evaluates a history course at Brooklyn College constructed around Benjamin Bloom's taxonomy of cognitive educational objectives. (Author/DB)

  9. Financial history and financial economics

    OpenAIRE

    Turner, John D.

    2014-01-01

    This essay looks at the bidirectional relationship between financial history and financial economics. It begins by giving a brief history of financial economics by outlining the main topics of interest to financial economists. It then documents and explains the increasing influence of financial economics upon financial history, and warns of the dangers of applying financial economics unthinkingly to the study of financial history. The essay proceeds to highlight the many insights that financi...

  10. Quantum histories without contrary inferences

    Energy Technology Data Exchange (ETDEWEB)

    Losada, Marcelo, E-mail: marcelolosada@yahoo.com [Instituto de Física Rosario, Pellegrini 250, 2000 Rosario (Argentina); Laura, Roberto, E-mail: rlaura@fceia.unr.edu.ar [Facultad de Ciencias Exactas, Ingeniería y Agrimensura and Instituto de Física Rosario, Pellegrini 250, 2000 Rosario (Argentina)

    2014-12-15

    In the consistent histories formulation of quantum theory it was shown that it is possible to retrodict contrary properties. We show that this problem do not appear in our formalism of generalized contexts for quantum histories. - Highlights: • We prove ordinary quantum mechanics has no contrary properties. • Contrary properties in consistent histories are reviewed. • We prove generalized contexts for quantum histories have no contrary properties.

  11. Financial history and financial economics

    OpenAIRE

    Turner, John D.

    2014-01-01

    This essay looks at the bidirectional relationship between financial history and financial economics. It begins by giving a brief history of financial economics by outlining the main topics of interest to financial economists. It then documents and explains the increasing influence of financial economics upon financial history, and warns of the dangers of applying financial economics unthinkingly to the study of financial history. The essay proceeds to highlight the many insights that financi...

  12. DNA methylation and not H3K4 trimethylation dictates the expression status of miR-152 gene which inhibits migration of breast cancer cells via DNMT1/CDH1 loop.

    Science.gov (United States)

    Sengupta, Dipta; Deb, Moonmoon; Rath, Sandip Kumar; Kar, Swayamsiddha; Parbin, Sabnam; Pradhan, Nibedita; Patra, Samir Kumar

    2016-08-15

    MicroRNAs (miRNA) are small non-coding RNAs which targets most protein-coding transcripts (mRNA) and destroy them. Thus miRNA controls the abundance of those specific proteins and impact on developmental, physiological and pathological processes. Dysregulation of miRNA function thus may lead to various clinicopathological complications, including breast cancer. Silencing of miR-152 gene due to promoter DNA methylation alter the expression pattern of several other genes. E-cadherin (CDH1) forms the core of adherent junctions between surrounding epithelial cells, link with actin cytoskeleton and affects cell signaling. CDH1 gene is down regulated by promoter DNA methylation during cancer progression. In this investigation, we attempt to elucidate the correlation of miR-152 and CDH1 function, as it is well known that the loss of CDH1 function is one of the major reasons for cancer metastasis and aggressiveness of spreading. For the first time we have shown that loss of CDH1 expression is directly proportional to the loss of miR-152 function in breast cancer cells. mRNA and protein expression profile of DNMT1 implicate that miR-152 targets DNMT1 mRNA and inhibits its protein expression. Tracing the molecular marks on DNA and histone 3 for understanding the mechanism of gene regulation by ChIP analyses leads to a paradoxical result that shows DNA methylation adjacent to active histone marking (enrichment of H3K4me3) silence miR-152 gene. Further experiments revealed that DNMT1 plays crucial role for regulation of miR-152 gene. When DNMT1 protein function is blocked miR-152 expression prevails and destroys the mRNA of DNMT1; this molecular regulatory mechanism is creating a cyclic feedback loop, which is now focused as DNMT1/miR-152 switch for on/off of DNMT1 target genes. We discovered modulation of CDH1 gene expression by DNMT1/miR-152 switches. We have demonstrated further that DNMT1 down regulation mediated upregulation of CDH1 (hereafter, DNMT1/CDH1 loop) in

  13. Voices in History

    Directory of Open Access Journals (Sweden)

    Ivan Leudar

    2001-06-01

    Full Text Available Experiences of “hearing voices” nowadays usually count as verbal hallucinations and they indicate serious mental illness. Some are first rank symptoms of schizophrenia, and the mass media, at least in Britain, tend to present them as antecedents of impulsive violence. They are, however, also found in other psychiatric conditions and epidemiological surveys reveal that even individuals with no need of psychiatric help can hear voices, sometimes following bereavement or abuse, but sometimes for no discernible reason. So do these experiences necessarily mean insanity and violence, and must they be thought of as pathogenic hallucinations; or are there other ways to understand them and live with them, and with what consequences?One way to make our thinking more flexible is to turn to history. We find that hearing voices was always an enigmatic experience, and the people who had it were rare. The gallery of voice hearers is, though, distinguished and it includes Galilei, Bunyan and St Teresa. Socrates heard a daemon who guided his actions, but in his time this did not signify madness, nor was it described as a hallucination. Yet in 19th century French psychological medicine the daemon became a hallucination and Socrates was retrospectively diagnosed as mentally ill. This paper examines the controversies which surrounded the experience at different points in history as well as the practice of retrospective psychiatry. The conclusion reached on the basis of the historical materials is that the experience and the ontological status it is ascribed are not trans-cultural or trans-historic but situated both in history and in the contemporary conflicts.

  14. Ethnic Groups in History Textbooks.

    Science.gov (United States)

    Glazer, Nathan; Ueda, Reed

    Six popular high school American history textbooks are examined to address accusations of overcompensation by textbook publishers as a result of the raised ethnic consciousness of the 1970s. The textbooks are: "Our American Heritage" (Silver Burdett); "The Pageant of American History" (Allyn and Bacon); "A History of Our American Republic"…

  15. Build Skills by Doing History

    Science.gov (United States)

    Monte-Sano, Chauncey

    2012-01-01

    No Child Left Behind has profoundly limited the teaching of history over the past 10 years. Now, the Common Core State Standards offers an opportunity to reverse this decline by giving history a more prominent place in the school curriculum alongside literacy goals. Learning history and argumentative writing is key to developing analytical ways of…

  16. Treating Globalization in History Surveys

    Science.gov (United States)

    Stearns, Peter N.

    2003-01-01

    Globalization provides history teachers with an opportunity to link past to present in new ways and to test historical thinking. This is particularly true in world history surveys, but has relevance to Western civilization or United States history surveys as well. For globalization in turn, the historical perspective offers opportunities for more…

  17. Nationalist Histories and Multiethnic Classrooms.

    Science.gov (United States)

    Stanley, Timothy J.

    2002-01-01

    Canadians do not have a common history, despite the English-speaking Eurocentric narrative that passes for Canadian history. Rethinking the history curriculum, combined with innovative practices such as using primary historical documents, conducting heritage fairs, and encouraging students to research and produce their own heritage minutes, can…

  18. Cultural History and Cultural Materialism.

    Science.gov (United States)

    Berman, Ronald

    1990-01-01

    Historicism critiques cultural history and cultural materialism as a methodology for literary analysis. Questions the finality of interpretation, how original values change, and whether dramatic history implies actual history. Using Shakespearean plays, analyzes the power and politics of a play in relation to its audience; posits that cultural…

  19. Cultural History and Cultural Materialism.

    Science.gov (United States)

    Berman, Ronald

    1990-01-01

    Historicism critiques cultural history and cultural materialism as a methodology for literary analysis. Questions the finality of interpretation, how original values change, and whether dramatic history implies actual history. Using Shakespearean plays, analyzes the power and politics of a play in relation to its audience; posits that cultural…

  20. History + Mystery = Inquiring Young Historians

    Science.gov (United States)

    Kirchner, Jana; Helm, Allison; Pierce, Kristin; Galloway, Michele

    2011-01-01

    While social studies content about communities, neighborhood jobs, and maybe even some state history is taught in the early elementary grades, often the upper elementary grades are the first time students learn about the larger progression of history. How do teachers begin to teach the progression of U.S. history and the themes and questions that…