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Sample records for k-4 k-5 nm

  1. Unifiability in extensions of K4

    NARCIS (Netherlands)

    Gencer, Ç.; de Jongh, D.

    2009-01-01

    We extend and generalize the work on unifiability of [8]. We give a semantic characterization for unifiability and non-unifiability in the extensions of K4. We apply this in particular to extensions of KD4, GL and K4.3 to obtain a syntactic characterization and give a concrete decision procedure for

  2. Optimizing Your K-5 Engineering Design Challenge

    Science.gov (United States)

    Coppola, Matthew Perkins; Merz, Alice H.

    2017-01-01

    Today, elementary school teachers continue to revisit old lessons and seek out new ones, especially in engineering. Optimization is the process by which an existing product or procedure is revised and refined. Drawn from the authors' experiences working directly with students in grades K-5 and their teachers and preservice teachers, the…

  3. Optimizing Your K-5 Engineering Design Challenge

    Science.gov (United States)

    Coppola, Matthew Perkins; Merz, Alice H.

    2017-01-01

    Today, elementary school teachers continue to revisit old lessons and seek out new ones, especially in engineering. Optimization is the process by which an existing product or procedure is revised and refined. Drawn from the authors' experiences working directly with students in grades K-5 and their teachers and preservice teachers, the…

  4. A pharmacological profile of the high-affinity GluK5 kainate receptor

    DEFF Research Database (Denmark)

    Møllerud, Stine; Kastrup, Jette Sandholm Jensen; Pickering, Darryl S

    2016-01-01

    Mouse GluK5 was expressed in Sf9 insect cells and radiolabelled with [3H]-kainate in receptor binding assays (Kd = 6.9 nM). Western immunoblotting indicated an Sf9 GluK5 band doublet corresponding to the glycosylated (128 kDa) and deglycosylated (111 kDa) protein, which was identical to the band...

  5. Teaching Strategies for K-4 General Music.

    Science.gov (United States)

    Teaching Music, 1999

    1999-01-01

    Provides a strategy, from the book entitled "Strategies for Teaching K-4 General Music," that addresses Standard 6A of the National Standards for Music Education. Explains that students will identify characteristics that enables them to make decisions about the form of the composition in listening examples longer than classroom songs. (CMK)

  6. A pharmacological profile of the high-affinity GluK5 kainate receptor.

    Science.gov (United States)

    Møllerud, Stine; Kastrup, Jette Sandholm; Pickering, Darryl S

    2016-10-05

    Mouse GluK5 was expressed in Sf9 insect cells and radiolabelled with [(3)H]-kainate in receptor binding assays (Kd=6.9nM). Western immunoblotting indicated an Sf9 GluK5 band doublet corresponding to the glycosylated (128kDa) and deglycosylated (111kDa) protein, which was identical to the band pattern of native rat brain GluK5. A pharmacological profile of the high-affinity kainate receptor GluK5 is described which is distinct from the profiles of other kainate receptors (GluK1-3). The 27 tested ligands generally show a preferential affinity to GluK1 over GluK5, the exceptions being: dihydrokainate, (S)-5-fluorowillardiine, (S)-glutamate and quisqualate, where the affinity is similar at GluK1 and GluK5. In contrast, quisqualate shows 40-fold higher affinity at GluK5 over GluK3 whereas (S)-1-(2'-amino-2'-caboxyethyl)thienol[3,4-d]pyrimidin-2,4-dione (NF1231), (RS)-2-amino-3-(5-tert-butyl-3-hydroxyisoxazol-4-yl)propionate (ATPA), dihydrokainate and (2S,4R)-4-methyl-glutamate (SYM2081) have higher affinity at GluK3 compared to GluK5. Since some studies have indicated that GluK5 is associated with various diseases in the central nervous system (e.g. schizophrenia, temporal lobe epilepsy, bipolar disorder), selective GluK5 ligands could have therapeutic potential. The distinct pharmacological profile of GluK5 suggests that it would be possible to design ligands with selectivity towards GluK5.

  7. ESSEA K-4 Online Course: Polar Connections

    Science.gov (United States)

    Blaney, L.; Myers, R. J.; Schwerin, T.

    2007-12-01

    The Earth System Science Education Alliance (ESSEA) is a National Science Foundation-supported program implemented by the Institute for Global Environmental Strategies (IGES) to improve the quality of geoscience instruction for pre-service, middle, and high school teachers. ESSEA increases teachers' access to quality materials, standards-based instructional methods and content knowledge. Started in 2000 and based on a trio of online courses (for elementary, middle, and high school teachers), the courses have been used by 40 faculty at 20 institutions educating over 1,700 teachers in Earth system science. Program evaluation of original course participants indicated that the courses had significant impact on teachers Earth system content knowledge and beliefs about teaching and learning. Seventeen of the original participating institutions have continued to use the courses and many have developed new programs that incorporate the courses in Earth science education opportunities for teachers. Today the ESSEA program lists nearly 40 colleges and universities as participants. The original K-4 course and modules have been revised to include topics and resources focusing on the International Polar Year. The new K-4 Land, Living Things, Water and Air modules contain inquiry-based investigations exploring our polar regions. Each module lists a set of essential questions that guide teachers and their students as they build content knowledge. The course structure requires teachers to work individually and in teams to build content knowledge and pedagogical understanding of how their students learn. This group investigation approach and a "Teacher as Researcher" theme promote reflection and collaboration to develop criteria for effective concept building. By exploring the characteristics of polar landscapes, atmosphere, and polar life, teachers and their students will develop new understandings about the interactions and dependencies of the Earth spheres and our polar regions

  8. Gifted Education and National Standards: A K-5 Program Evaluation

    Science.gov (United States)

    Harwell-Braun, Debra A.

    2010-01-01

    The purpose of this study was to conduct a program evaluation of a K-5 Gifted Education Program. Program evaluation addressed how well the gifted education program studied met the National Association of Gifted Children standards. In addition, this study included stakeholder perceptions of the current gifted education program K-5. This program…

  9. On the Crossing Numbers of K5×Sn%K5×Sn的交叉数

    Institute of Scientific and Technical Information of China (English)

    吕胜祥; 黄元秋

    2008-01-01

    By connecting the 5 vertices of K5 to other n vertices, we obtain a special family of graph denoted by Hn. This paper proves that the crossing number of Hn is Z(5, n) +2n+[n/2] 1, and the crossing number of Cartesian products of K5 with star Sn is Z(5, n) + 5n + [n/2]+1.

  10. Parity, cycle space, and K4-subdivisions in graphs

    DEFF Research Database (Denmark)

    Thomassen, Carsten

    1999-01-01

    . Zang). We prove the new result that every $4$-connected graph with at least three triangles contains a totally odd $K_4$-subdivision if and only if it does notcontain a vertex whose deletion results in a bipartite graph.In particular, every $4$-connected planar graph contains a totally odd $K_4...

  11. Peaked and Smooth Solitons for K*(4,1 Equation

    Directory of Open Access Journals (Sweden)

    Yongan Xie

    2013-01-01

    Full Text Available This paper is contributed to explore all possible single peak solutions for the K*(4,1 equation ut=uxu2+2α(uuxxx+2uxuxx. Our procedure shows that the K*(4,1 equation either has peakon, cuspon, and smooth soliton solutions when sitting on a nonzero constant pedestal limξ→±∞u=A≠0 or possesses compacton solutions only when limξ→±∞u=A=0. We present a new smooth soliton solution in an explicit form. Mathematical analysis and numeric graphs are provided for those soliton solutions of the K*(4,1 equation.

  12. Juneau Indian Studies Elementary Curriculum Guide. Grades K-5.

    Science.gov (United States)

    Cadiente, Ronalda

    Designed to provide instruction in Tlingit culture as an integral part of the K-5 social studies curriculum, this guide presents teachers with extensive lesson plans and numerous resource materials. The units of study focus on the culture and environment of southeast Alaska and emphasize experiential learning activities. Each grade…

  13. Math and Economics: Implementing Authentic Instruction in Grades K-5

    Science.gov (United States)

    Althauser, Krista; Harter, Cynthia

    2016-01-01

    The purpose of this study is to outline a partnership program that involved a local elementary school district, an institution of higher education, the local business community, and a state economic education advocacy group to integrate economics into math in grades K-5. The "Economics: Math in Real Life" program was provided in…

  14. The Common Core: Insights into the K-5 Standards

    Science.gov (United States)

    McLaughlin, Maureen; Overturf, Brenda J.

    2012-01-01

    The role of the Common Core English Language Arts Standards in grades K-5 literacy instruction is the focus of the article. The authors begin by raising four questions: (1) What is the essential philosophy of the Common Core State Standards?; (2) What do educators need to know to use the College and Career Readiness Standards and Common Core State…

  15. Phosphorus K4 Crystal: A New Stable Allotrope

    Science.gov (United States)

    Liu, Jie; Zhang, Shunhong; Guo, Yaguang; Wang, Qian

    2016-11-01

    The intriguing properties of phosphorene motivate scientists to further explore the structures and properties of phosphorus materials. Here, we report a new allotrope named K4 phosphorus composed of three-coordinated phosphorus atoms in non-layered structure which is not only dynamically and mechanically stable, but also possesses thermal stability comparable to that of the orthorhombic black phosphorus (A17). Due to its unique configuration, K4 phosphorus exhibits exceptional properties: it possesses a band gap of 1.54 eV which is much larger than that of black phosphorus (0.30 eV), and it is stiffer than black phosphorus. The band gap of the newly predicted phase can be effectively tuned by appling hydrostastic pressure. In addition, K4 phosphorus exibits a good light absorption in visible and near ultraviolet region. These findings add additional features to the phosphorus family with new potential applications in nanoelectronics and nanomechanics.

  16. Properties of atomic intercalated carbon K4 crystals

    OpenAIRE

    Itoh, Masahiro; Takami, Seiichi; Kawazoe, Yoshiyuki; Adschiri, Tadafumi

    2009-01-01

    The stability of atomic intercalated carbon $K_{4}$ crystals, XC$_{2}$ (X=H, Li, Be, B, C, N, O, F, Na, Mg, Al, Si, P, S, Cl, K, Ca, Ga, Ge, As, Se, Br, Rb or Sr) is evaluated by geometry optimization and frozen phonon analysis based on first principles calculations. Although C $K_{4}$ is unstable, NaC$_{2}$ and MgC$_{2}$ are found to be stable. It is shown that NaC$_{2}$ and MgC$_{2}$ are metallic and semi conducting, respectively.

  17. The existence spectrum of Meta(K4(3)>K4(3)-e)

    Institute of Scientific and Technical Information of China (English)

    LO; FARO; Giovanni; TRIPODI; Antoinette

    2010-01-01

    Let H and J1 be both t-uniform hypergraphs. Let J2 be a sub-hypergraph of J1. In this paper, the metamorphosis of a hypergraph decomposition is introduced, denoted by (H, J1 > J2)-design, which is a generalization of the concept of metamorphosis of a graph decomposition. Let Meta(J1>J2) denote the set of all integers v such that there exists a (Kv((3)), J1>J2)-design. We completely determine the set Meta(K4((3))>K4((3))-e).

  18. The Role of GluK4 in Synaptic Plasticity and Affective Behavior in Mice

    Science.gov (United States)

    Catches, Justin Samuel

    Kainate receptors (KARs) are glutamate-gated ion channels that signal through both ionotropic and metabotropic pathways (Contractor et al., 2011). Combinations of five KAR subunits (GluK1-5) form tetrameric receptors with GluK1, GluK2, and GluK3 able to form functional homomeric channels. The high-affinity subunits, GluK4 and GluK5, do not form homomeric channels but modify the properties of heteromeric receptors. Expression of the GluK4 receptor subunit in the forebrain is restricted to the CA3 region of the hippocampus and dentate gyrus regions where KARs modulate synaptic plasticity. In this study, ablation of Grik4, which encodes GluK4, in mice reduced KAR synaptic currents and altered activation properties of postsynaptic receptors but left two forms of presynaptic short-term plasticity intact. Disruption of both Grik4 and Grik5 caused complete loss of the postsynaptic ionotropic KAR current and impaired presynaptic frequency facilitation. Additionally, KAR surface expression was altered at pre- and postsynaptic sites at the MF synapse. Despite the loss of ionotropic signaling, KAR-mediated inhibition of the slow afterhyperpolarization current, which is dependent on metabotropic signaling, was intact in CA3 neurons. Long-term potentiation at the MF-CA3 synapse was reduced, likely through a loss of KAR modulation of excitability of the presynaptic MF axons. Genetic variants in the human GRIK4 gene alter the susceptibility for affective disorders (Bloss and Hunter, 2010). We found that ablation of Grik4 in mice resulted in reduced anxiety and an antidepressant-like phenotype. In the elevated zero-maze, a test for anxiety and risk taking behavior, and in two anxiogenic tests, marble-burying and novelty-induced suppression of feeding, anxiety-like behavior was consistently reduced in knockout animals. In the forced swim, a test of learned helplessness used to determine depression-like behavior, knockout mice demonstrated significantly less immobility suggesting

  19. CCM key comparison CCM.D-K4 'Hydrometer'

    Science.gov (United States)

    Lorefice, S.; Becerra, L. O.; Lenard, E.; Lee, Y. J.; Lee, W. G.; Madec, T.; Meury, P. A.; Caceres, J.; Santos, C.; Vamossy, C.; Man, J.; Fen, K.; Toda, K.; Wright, J.; Bettin, H.; Toth, H.

    2016-01-01

    This report presents the comparison philosophy, methodology, analysis and the results of the designed CCM.D-K4 key comparison that covered the calibration of high resolution hydrometers for liquid and alcoholometers in the density range 600 kg/m3 to 2000 kg/m3 at the temperature of 20°C. The main purpose of this comparison was not only to evaluate the degree of equivalence in the calibration of high accuracy hydrometers between NMI participants, but also to link, were it is possible, the results of previous comparisons to Key Comparison Reference Values (KCRVs) of CCM.D-K4. Eleven NMI laboratories took part in the CCM.D-K4 divided in two groups (petals). With the CCM.D-K4 purpose, two similar sets consisting of three hydrometers for liquid density determinations and an alcoholometer were circulated to the NMI participants as a travelling standard in the time interval from January 2011 to April 2012. Twelve Key Comparison Reference Values (KCRVs) for each petal have been obtained at the density values related to the tested density marks of the transfer standards by the results of participants. The KCRVs and corresponding uncertainties were calculated by the weighted mean in the case of consistent results, otherwise the median was used. The degree of equivalence (DoE) with respect to the corresponding KCRV was determined for each participant and, in this particular comparison, the Weighted Least Squares (WLS) method was used to link the individual DoE of each participant by a continuous function. Significant drift of the transfer standards was not detected. This report also gives instructions on calculating pair-wise degrees of equivalence, with the addition of any information on correlations that may be necessary to estimate more accurately as well as the procedure for linking international comparisons to the CCM.D-K4. Finally an example of linkage to the CCM.D-K4 is given by dealing with the results of the bilateral comparison between INRiM and NMIA, which was

  20. Properties of atomic intercalated boron nitride K4 type crystals

    OpenAIRE

    Itoh, Masahiro; Takami, Seiichi; Kawazoe, Yoshiyuki; Adschiri, Tadafumi

    2010-01-01

    The stability of atomic intercalated boron nitride K4 crystal structures, XBN (X=H, Li, Be, B, C, N, O, F, Na, Mg, Al, Si, P, S, Cl, K, Ca, Ga, Ge, As, Se, Br, Rb or Sr) is evaluated by the geometric optimization and frozen phonon calculations based on the first principles calculations. NaBN, MgBN, GaBN, FBN and ClBN are found to be stable. NaBN, GaBN, FBN and ClBN are metallic, whereas MgBN is semiconducting.

  1. The Epstein-Barr Virus-encoded miR-BART22 targets MAP3K5 to promote host cell proliferative and invasive abilities in nasopharyngeal carcinoma

    Science.gov (United States)

    Chen, Ruichao; Zhang, Minfeng; Li, Qiulian; Xiong, Hanzhen; Liu, Shaoyan; Fang, Weiyi; Zhang, Qianbing; Liu, Zhen; Xu, Xuehu; Jiang, Qingping

    2017-01-01

    miR-BART22, a new discovered Epstein-Barr virus (EBV) miRNA, is abundant in Nasopharyngeal carcinoma (NPC). It has been reported that miR-BART22 promoted the tumor development by down-modulating EBV LMP2 expression to evade the host immune response. But its cell target genes have still been obscure. We have reported an inverse correlation between the BART-22 and MAP3K5 protein expression in NPC tissues and NPC cell lines. Meanwhile, MAP3K5 protein expression level was significantly decreased in primary NPC tissues compared with nasopharyngitis when MAP3K5 mRNA expression was consistent in two group tissues. According to our data and target prediction by miRnada, we assume MAP3K5 is an important target gene of NPC. MAP3K5, also named apoptosis signal-regulating kinase1 (ASK1), is an important early answer gene in P38MAPK pathway and an apoptosis-related gene. In present study, MAP3K5 was verified the target gene of miR-BART22 by luciferase assay. miRBART-22 decreased MAP3K5 protein level. Moreover, it also decreased MAP3K5 downstream gene MAP2K4 expression in P38MAPK pathway, and even their activated phosphorylation forms. Additionally, we found stable transfection of miR-BAT22 could improve tumor cells' proliferative and invasive abilities in NPC cell line 5-8F. The data highlight the role of the EBV miR-BART22 in regulating genes involving in apoptosis and some important pathways to promote cancer development. And it also raises the possibility that inhibitors of miR-BART22 can be as a therapeutic strategy for NPC and other EBV-infected tumors treatment. PMID:28243335

  2. Reading, Writing & Rings: Science Literacy for K-4 Students

    Science.gov (United States)

    McConnell, S.; Spilker, L.; Zimmerman-Brachman, R.

    2007-12-01

    Scientific discovery is the impetus for the K-4 Education program, "Reading, Writing & Rings." This program is unique because its focus is to engage elementary students in reading and writing to strengthen these basic academic skills through scientific content. As science has been increasingly overtaken by the language arts in elementary classrooms, the Cassini Education Program has taken advantage of a new cross-disciplinary approach to use language arts as a vehicle for increasing scientific content in the classroom. By utilizing the planet Saturn and the Cassini-Huygens mission as a model in both primary reading and writing students in these grade levels, young students can explore science material while at the same time learning these basic academic skills. Content includes reading, thinking, and hands-on activities. Developed in partnership with the Cassini-Huygens Education and Public Outreach Program, the Bay Area Writing Project/California Writing Project, Foundations in Reading Through Science & Technology (FIRST), and the Caltech Pre-College Science Initiative (CAPSI), and classroom educators, "Reading, Writing & Rings" blends the excitement of space exploration with reading and writing. All materials are teacher developed, aligned with national science and language education standards, and are available from the Cassini-Huygens website: http://saturn.jpl.nasa.gov/education/edu-k4.cfm Materials are divided into two grade level units. One unit is designed for students in grades 1 and 2 while the other unit focuses on students in grades 3 and 4. Each includes a series of lessons that take students on a path of exploration of Saturn using reading and writing prompts.

  3. 26 CFR 1.401(k)-5 - Special rules for mergers, acquisitions and similar events. [Reserved

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 5 2010-04-01 2010-04-01 false Special rules for mergers, acquisitions and similar events. 1.401(k)-5 Section 1.401(k)-5 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE.... § 1.401(k)-5 Special rules for mergers, acquisitions and similar events....

  4. The Amazing COS FUV (1320 - 1460 A) Spectrum of (lambda) Vel (K4Ib-II)

    Science.gov (United States)

    Carpenter, Kenneth

    2010-01-01

    The FUV spectrum (1320-1460 A) of the K4 lb-11 supergiant (lambda) Vel was observed with the Cosmic Origins Spectrograph (COS) on HST, as part of the Ayres and Redfield Cycle 17 SNAP program "SNAPing Coronal Iron". This spectrum covers a region not previously recorded in (lambda) Vel at high resolution and, in a mere 20 minutes of exposure, reveals a treasure trove of information. It shows a wide variety of strong emission lines, superposed on a bright continuum, with contributions from both atomic and molecular species. Multiple absorptions, including numerous Ni II and Fe II lines, are visible over this continuum, which is likely generated in the chromosphere of the star. Evidence of the stellar wind is seen in the P Cygni profiles of the CII lines near 1335 A and the results of fluorescence processes are visible throughout the region. The spectrum has remarkable similarities to that of (alpha) Boo (K1.5 III), but significant differences as well, including substantial FUV continuum emission, reminiscent of the M2 Iab supergiant (alpha) Ori, but minus the CO fundamental absorption bands seen in the spectrum of the latter star. However, fluoresced CO emission is present, as in the K-giant stars (alpha) Boo and (alpha) Tau (K5 III). The presence of hot plasma in the atmosphere of the star, indicated by previous GHRS observations of Si III] and C III] lines near 1900 A and FUSE observations of O VI 1032 A, is further confirmed by the detection in this COS spectrum of the Si IV UV 1 lines near 1400 A, though both lines are contaminated by overlying fluorescent H2 emission. We present the details of this spectrum, in comparison with stars of similar temperature or luminosity and discuss the implications for the structure of, and the radiative processes active in, the outer atmospheres of these stars.

  5. Interaction of Escherichia coli K1 and K5 with Acanthamoeba castellanii trophozoites and cysts.

    Science.gov (United States)

    Matin, Abdul; Jung, Suk-Yul

    2011-12-01

    The existence of symbiotic relationships between Acanthamoeba and a variety of bacteria is well-documented. However, the ability of Acanthamoeba interacting with host bacterial pathogens has gained particular attention. Here, to understand the interactions of Escherichia coli K1 and E. coli K5 strains with Acanthamoeba castellanii trophozoites and cysts, association assay, invasion assay, survival assay, and the measurement of bacterial numbers from cysts were performed, and nonpathogenic E. coli K12 was also applied. The association ratio of E. coli K1 with A. castellanii was 4.3 cfu per amoeba for 1 hr but E. coli K5 with A. castellanii was 1 cfu per amoeba for 1 hr. By invasion and survival assays, E. coli K5 was recovered less than E. coli K1 but still alive inside A. castellanii. E. coli K1 and K5 survived and multiplied intracellularly in A. castellanii. The survival assay was performed under a favourable condition for 22 hr and 43 hr with the encystment of A. castellanii. Under the favourable condition for the transformation of trophozoites into cysts, E. coli K5 multiplied significantly. Moreover, the pathogenic potential of E. coli K1 from A. castellanii cysts exhibited no changes as compared with E. coli K1 from A. castellanii trophozoites. E. coli K5 was multiplied in A. castellanii trophozoites and survived in A. castellanii cysts. Therefore, this study suggests that E. coli K5 can use A. castellanii as a reservoir host or a vector for the bacterial transmission.

  6. Survivability of Psychrobacter cryohalolentis K5 Under Simulated Martian Surface Conditions

    Science.gov (United States)

    Smith, David J.; Schuerger, Andrew C.; Davidson, Mark M.; Pacala, Stephen W.; Bakermans, Corien; Onstott, Tullis C.

    2009-03-01

    Spacecraft launched to Mars can retain viable terrestrial microorganisms on board that may survive the interplanetary transit. Such biota might compromise the search for life beyond Earth if capable of propagating on Mars. The current study explored the survivability of Psychrobacter cryohalolentis K5, a psychrotolerant microorganism obtained from a Siberian permafrost cryopeg, under simulated martian surface conditions of high ultraviolet irradiation, high desiccation, low temperature, and low atmospheric pressure. First, a desiccation experiment compared the survival of P. cryohalolentis cells embedded, or not embedded, within a medium/salt matrix (MSM) maintained at 25°C for 24 h within a laminar flow hood. Results indicate that the presence of the MSM enhanced survival of the bacterial cells by 1 to 3 orders of magnitude. Second, tests were conducted in a Mars Simulation Chamber to determine the UV tolerance of the microorganism. No viable vegetative cells of P. cryohalolentis were detected after 8 h of exposure to Mars-normal conditions of 4.55 W/m2 UVC irradiation (200-280 nm), -12.5°C, 7.1 mbar, and a Mars gas mix composed of CO2 (95.3%), N2 (2.7%), Ar (1.6%), O2 (0.2%), and H2O (0.03%). Third, an experiment was conducted within the Mars chamber in which total atmospheric opacities were simulated at τ = 0.1 (dust-free CO2 atmosphere at 7.1 mbar), 0.5 (normal clear sky with 0.4 = dust opacity and 0.1 = CO2-only opacity), and 3.5 (global dust storm) to determine the survivability of P. cryohalolentis to partially shielded UVC radiation. The survivability of the bacterium increased with the level of UVC attenuation, though population levels still declined several orders of magnitude compared to UVC-absent controls over an 8 h exposure period.

  7. Mitogen-activated protein kinase kinase 4 (MAP2K4 promotes human prostate cancer metastasis.

    Directory of Open Access Journals (Sweden)

    Janet M Pavese

    Full Text Available Prostate cancer (PCa is the second leading cause of cancer death in the US. Death from PCa primarily results from metastasis. Mitogen-activated protein kinase kinase 4 (MAP2K4 is overexpressed in invasive PCa lesions in humans, and can be inhibited by small molecule therapeutics that demonstrate favorable activity in phase II studies. However, MAP2K4's role in regulating metastatic behavior is controversial and unknown. To investigate, we engineered human PCa cell lines which overexpress either wild type or constitutive active MAP2K4. Orthotopic implantation into mice demonstrated MAP2K4 increases formation of distant metastasis. Constitutive active MAP2K4, though not wild type, increases tumor size and circulating tumor cells in the blood and bone marrow. Complementary in vitro studies establish stable MAP2K4 overexpression promotes cell invasion, but does not affect cell growth or migration. MAP2K4 overexpression increases the expression of heat shock protein 27 (HSP27 protein and protease production, with the largest effect upon matrix metalloproteinase 2 (MMP-2, both in vitro and in mouse tumor samples. Further, MAP2K4-mediated increases in cell invasion are dependent upon heat shock protein 27 (HSP27 and MMP-2, but not upon MAP2K4's immediate downstream targets, p38 MAPK or JNK. We demonstrate that MAP2K4 increases human PCa metastasis, and prolonged over expression induces long term changes in cell signaling pathways leading to independence from p38 MAPK and JNK. These findings provide a mechanistic explanation for human studies linking increases in HSP27 and MMP-2 to progression to metastatic disease. MAP2K4 is validated as an important therapeutic target for inhibiting human PCa metastasis.

  8. K4Sn3Se8的固相合成、晶体结构和反射光谱研究%Solid state synthesis,crystal structure and diffuse reflectance spectra of K4Sn3Se8

    Institute of Scientific and Technical Information of China (English)

    王志霞; 郭洪猷

    2003-01-01

    采用反应性熔盐法,通过中温固相反应在500 ℃下合成晶体K4Sn3Se8.该晶体属正交晶系,空间群Ccca,晶胞参数:a=0.821 15(6) nm,b=2.779 0(2) nm,c=0.818 65(6) nm,V=1.868 2(2) nm3,Z=4.K4Sn3Se8由[Sn3Se8]4-三聚体负离子和K+正离子组成.漫反射光谱研究表明该材料能隙(Eg)为2.6 eV,属于半导体,对太阳能具有选择吸收的特性.

  9. Impaired removal of H3K4 methylation affects cell fate determination and gene transcription

    DEFF Research Database (Denmark)

    Lussi, Yvonne C; Mariani, Luca; Rundsten, Carsten Friis;

    2016-01-01

    implicated in development, but how the regulation of H3K4me3 level controls developmental processes is not fully established. Here, we show that the H3K4 demethylase RBR-2, the unique member of the KDM5 family in C. elegans, acts cell-autonomously and in a catalytic-dependent manner to control vulva...

  10. The GluK4 kainate receptor subunit regulates memory, mood, and excitotoxic neurodegeneration.

    Science.gov (United States)

    Lowry, E R; Kruyer, A; Norris, E H; Cederroth, C R; Strickland, S

    2013-04-01

    Though the GluK4 kainate receptor subunit shows limited homology and a restricted expression pattern relative to other kainate receptor subunits, its ablation results in distinct behavioral and molecular phenotypes. GluK4 knockout mice demonstrated impairments in memory acquisition and recall in a Morris water maze test, suggesting a previously unreported role for kainate receptors in spatial memory. GluK4 knockout mice also showed marked hyperactivity and impaired pre-pulse inhibition, thereby mirroring two of the hallmark endophenotypes of patients with schizophrenia and bipolar disorder. Furthermore, we found that GluK4 is a key mediator of excitotoxic neurodegeneration: GluK4 knockout mice showed robust neuroprotection in the CA3 region of the hippocampus following intrahippocampal injection of kainate and widespread neuroprotection throughout the hippocampus following hypoxia-ischemia. Biochemical analysis of kainate- or sham-treated wild-type and GluK4 knockout hippocampal tissue suggests that GluK4 may act through the JNK pathway to regulate the molecular cascades that lead to excitotoxicity. Together, our findings suggest that GluK4 may be relevant to the understanding and treatment of human neuropsychiatric and neurodegenerative disorders.

  11. [Synthesis, spectral analysis and photocatalysis of Ag/K4Nb6,O17 heterojunction catalysts].

    Science.gov (United States)

    Zhang, Feng-li; Cao, Yan-ning; Ying, Song; Chen, Rong; Zhang, Han-hui; Zheng, Qi

    2010-10-01

    K4Nb6O17 photocatalyst was successfully synthesized by low-temperature hydrothermal method with layer structure. Considering that a large number of hydroxyl (Nb-OH) and oxygen species (Nb==O, Nb--O-) exist on the surface of K4Nb6O17 synthesized by hydrothermal method, Ag(en)2+ precursors were employed to synthesize Ag/K4Nb6O17 heterostructure photo-catalysts with highly dispersed Ag. Photocatalytic performance evaluation results show that the photodegradation rate of MO for K4Nb6O17 was remarkably improved when a small amont of Ag was loaded. The best loading dose of Ag is 0.5 at%. Based on various characterizations results of XRD, FTIR, UV-Vis DRS, XRF and TEM, the photocatalytic mechanism of Ag/ K4Nb6O17 heterostructure catalysts was illuminated in detail and the conclusions were drawn as follows: (1) K4Nb6O17 nanocrystals serve as electron and hole sources for degradation of an organic dye; (2) Ag nanoparticles on the surface of K4Nb6O17 nanocrystals act as a sink for the electrons, promote interfacial charge-transfer kinetics between the metal and semiconductor, improve the separation of photogenerated electron-hole pairs, and thus enhance the photocatalytic activity of Ag/K4Nb6O17 photocatalyst.

  12. Assembly Stoichiometry of the GluK2/GluK5 Kainate Receptor Complex

    Directory of Open Access Journals (Sweden)

    Andreas Reiner

    2012-03-01

    Full Text Available Ionotropic glutamate receptors assemble as homo- or heterotetramers. One well-studied heteromeric complex is formed by the kainate receptor subunits GluK2 and GluK5. Retention motifs prevent trafficking of GluK5 homomers to the plasma membrane, but coassembly with GluK2 yields functional heteromeric receptors. Additional control over GluK2/GluK5 assembly seems to be exerted by the amino-terminal domains, which preferentially assemble into heterodimers as isolated domains. However, the stoichiometry of the full-length GluK2/GluK5 receptor complex has yet to be determined, as is the case for all non-NMDA glutamate receptors. Here, we address this question, using a single-molecule imaging technique that enables direct counting of the number of each GluK subunit type in homomeric and heteromeric receptors in the plasma membranes of live cells. We show that GluK2 and GluK5 assemble with 2:2 stoichiometry. This is an important step toward understanding the assembly mechanism, architecture, and functional consequences of heteromer formation in ionotropic glutamate receptors.

  13. DVIG ENERGETSKE UČINKOVITOSTI PREMOGOVNEGA KOTLA K4 V TOVARNI VIPAP

    OpenAIRE

    Lekše, Aleš

    2014-01-01

    Diplomsko delo se nanaša na znižanje lastne rabe energije na postrojenju kotla K4 ter na dvig deleža pridobljene energije iz OVE v podjetju Vipap Videm Krško. Na podlagi ugotovitev, da so izvedeni ukrepi znižali lastno rabo električne energije, večji delež OVE v strukturi goriva pa je zagotovil željeno toplotno moč kotla K4, nam zaključni del naloge potrjuje, da so vsi izvedeni ukrepi upravičili ekonomsko in ekološko upravičenost posodobitve postrojenja kotla K4.

  14. On a $\\{K_4,L(Q_3)\\}_{K_3}$-ultrahomogeneous graph

    CERN Document Server

    Dejter, Italo J

    2010-01-01

    A construction based on the Biggs-Smith graph is shown to produce an edge-disjoint union of 102 copies of $K_4$ forming a $\\{K_4,L(Q_3)\\}_{K_3}$-ultrahomogeneous graph as well as the non-line-graphical Menger graph of a self-dual $(102_4)$-configuration. This stands in contrast to and is a follow-up of the self-dual $(42_4)$-configuration of \\cite{D1}, whose Menger graph is a non-line-graphical $\\{K_4,$ $K_{2,2,2}\\}_{K_2}$-ultrahomogeneous graph.

  15. Multifrequency Study of Giant Radio Pulses from the Crab Pulsar with K5 VLBI Recording Terminal

    CERN Document Server

    Popov, M V; Kondratiev, V I; Bilous, A V; Moshkina, O; Oreshko, V V; Ilyasov, Yu P; Sekido, M; Kondo, T

    2009-01-01

    Simultaneous multifrequency observations of the Crab pulsar giant pulses (GPs) were performed with the 64-m Kalyazin radio telescope at four frequencies 0.6, 1.4, 2.2 and 8.3 GHz using the K5 VLBI recording terminal. K5 terminal provided continuous recording in 16 4-MHz wide frequency channels distributed over 4 frequency bands. Several thousands of GPs were detected during about 6 hours of observations in two successive days in July 2005. Radio spectra of single GPs were analysed at separate frequencies and over whole frequency range. These spectra manifest notable modulation both on large ($\\Delta\

  16. Effects of nickel treatment on H3K4 trimethylation and gene expression.

    Directory of Open Access Journals (Sweden)

    Kam-Meng Tchou-Wong

    Full Text Available Occupational exposure to nickel compounds has been associated with lung and nasal cancers. We have previously shown that exposure of the human lung adenocarcinoma A549 cells to NiCl(2 for 24 hr significantly increased global levels of trimethylated H3K4 (H3K4me3, a transcriptional activating mark that maps to the promoters of transcribed genes. To further understand the potential epigenetic mechanism(s underlying nickel carcinogenesis, we performed genome-wide mapping of H3K4me3 by chromatin immunoprecipitation and direct genome sequencing (ChIP-seq and correlated with transcriptome genome-wide mapping of RNA transcripts by massive parallel sequencing of cDNA (RNA-seq. The effect of NiCl(2 treatment on H3K4me3 peaks within 5,000 bp of transcription start sites (TSSs on a set of genes highly induced by nickel in both A549 cells and human peripheral blood mononuclear cells were analyzed. Nickel exposure increased the level of H3K4 trimethylation in both the promoters and coding regions of several genes including CA9 and NDRG1 that were increased in expression in A549 cells. We have also compared the extent of the H3K4 trimethylation in the absence and presence of formaldehyde crosslinking and observed that crosslinking of chromatin was required to observe H3K4 trimethylation in the coding regions immediately downstream of TSSs of some nickel-induced genes including ADM and IGFBP3. This is the first genome-wide mapping of trimethylated H3K4 in the promoter and coding regions of genes induced after exposure to NiCl(2. This study may provide insights into the epigenetic mechanism(s underlying the carcinogenicity of nickel compounds.

  17. Cellular Membrane Composition Requirement by Antimicrobial and Anticancer Peptide GA-K4.

    Science.gov (United States)

    Mishig-Ochir, Tsogbadrakh; Gombosuren, Davaadulam; Jigjid, Altanchimeg; Tuguldur, Badamkhatan; Chuluunbaatar, Galbadrakh; Urnukhsaikhan, Enerelt; Pathak, Chinar; Lee, Bong-Jin

    2017-01-01

    Naturally occurring antimicrobial peptides important for innate immunity are widely studied for their antimicrobial and anticancer activity. The primary target of these AMPs is believed to be the bacterial cytoplasmic membrane. However, the interaction between cytoplasmic membrane and the antimicrobial peptides remains poorly understood. Therefore to focus on the target membrane composition that is required by AMPs to interact with membranes, we have examined the interaction of the antimicrobial and anticancer active 11-residue GA-K4 (FLKWLFKWAKK) peptide with model and intact cell membranes. Effect on the structural conformational properties of GA-K4 peptide was investigated by means of far-UV CD and fluorescence spectroscopic methods. The different conformation of GA-K4 peptide in large unilamellar vesicles (LUV) bilayer and micelle environment suggest that the curvature has an influence on the secondary structure acquired by the peptide. Furthermore, the leakage experiment result confirmed that GA-K4 induced the leakage of cytoplasmic membrane in Staphylococcus аureus bacterial cells. Fluorescence data revealed the interfacial location of GA-K4 peptide in the model membranes. The blue-shift in emission wavelength by tryptophan residues in fluorescence data indicated the penetration of GA-K4 peptide in micelles and phospholipid bilayers. These results showed that the GA-K4 peptide is a membrane-active peptide and its activity depends on membrane curvature and lipid composition. Although further studies are required to confirm the mechanism of action, the data suggest mechanism of toroidal pore formation for the interaction of GA-K4 peptide with membranes. Our studies will be helpful in better understanding of the membrane requirment of peptides to express their therapeutic effects. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  18. CFP1 Regulates Histone H3K4 Trimethylation and Developmental Potential in Mouse Oocytes

    Directory of Open Access Journals (Sweden)

    Chao Yu

    2017-08-01

    Full Text Available Trimethylation of histone H3 at lysine-4 (H3K4me3 is associated with eukaryotic gene promoters and poises their transcriptional activation during development. To examine the in vivo function of H3K4me3 in the absence of DNA replication, we deleted CXXC finger protein 1 (CFP1, the DNA-binding subunit of the SETD1 histone H3K4 methyltransferase, in developing oocytes. We find that CFP1 is required for H3K4me3 accumulation and the deposition of histone variants onto chromatin during oocyte maturation. Decreased H3K4me3 in oocytes caused global downregulation of transcription activity. Oocytes lacking CFP1 failed to complete maturation and were unable to gain developmental competence after fertilization, due to defects in cytoplasmic lattice formation, meiotic division, and maternal-zygotic transition. Our study highlights the importance of H3K4me3 in continuous histone replacement for transcriptional regulation, chromatin remodeling, and normal developmental progression in a non-replicative system.

  19. Acyclic chromatic indices of K4-minor free graphs%没有K4-图子式的图的无圈边色数

    Institute of Scientific and Technical Information of China (English)

    王维凡; 舒巧君

    2011-01-01

    一个图G的无圈k-边染色是指G的一个正常的不产生双色圈的k-边染色.G的无圈边色数a1(G)定义为使得G有一个无圈k-边染色的最小的整数k.本文完全刻画了最大度不为4的没有K4-图子式的图的无圈边色数.%An acyclic edge coloring of a graph G is a proper edge coloring such that no Dichromatic cycles are produced. The acyclic chromatic index a'(G) of G is the smallest integer k such that G has an acyclic edge coloring using k colors. In this paper, we determine completely the acyclic chromatic indices of K4-minor free graphs with maximum degree not equal to 4.

  20. Mobile Devices and the Teacher Perceived Barriers Impacting Effective Integration in the K-5 Classroom

    Science.gov (United States)

    Nixon, Tina S.

    2013-01-01

    This qualitative, phenomenological study explored the teacher perceived barriers of using mobile devices in the K-5 classroom. Research confirms teachers face various types of variables and become reluctant to use technology within their curriculum driven lessons. This study sought to understand what teachers perceive as barriers, and how the…

  1. K-5 Student Experiences in a Dance Residency: A Case Study

    Science.gov (United States)

    Leonard, Alison E.; McShane-Hellenbrand, Karen

    2012-01-01

    In this article, the collaborating authors, a researcher and dance artist, confront assumptions surrounding dance's experiential nature and assessment in schools. Presenting findings from a qualitative case study assessment of a three-week, whole-school dance artist-in-residence at a diverse and inclusive metropolitan K-5 school, the authors focus…

  2. Democratic Bodies: Exemplary Practice and Democratic Education in a K-5 Dance Residency

    Science.gov (United States)

    Leonard, Alison E.

    2014-01-01

    This research highlights a K-5 dance artist-in-residence as a form of democratic and exemplary dance education that ignited collaboration, promoted equity, fostered student autonomy, and demonstrated rigor in school curriculum. Through examining observation, interview, and performance-based data and calling upon critical, democratic education…

  3. Democratic Bodies: Exemplary Practice and Democratic Education in a K-5 Dance Residency

    Science.gov (United States)

    Leonard, Alison E.

    2014-01-01

    This research highlights a K-5 dance artist-in-residence as a form of democratic and exemplary dance education that ignited collaboration, promoted equity, fostered student autonomy, and demonstrated rigor in school curriculum. Through examining observation, interview, and performance-based data and calling upon critical, democratic education…

  4. 油水自组装制备Ag@AgCl/K4Nb6 O17及其光催化降解有机污染物的研究∗%Oil-water self-assembly synthesis of Ag@AgCl/K4 Nb6 O17 and enhanced photocatalytic degradation of organic pollutants

    Institute of Scientific and Technical Information of China (English)

    赵俊秀; 刘利; 胡金山; 梁英华; 崔文权

    2015-01-01

    在高温固相法制备K4 Nb6 O17的基础上,采用油水自组装合成复合光催化剂 Ag @ AgCl/K4 Nb6 O17,利用 XRD、SEM、TEM、EDX、UV-Vis、PL、BET等多种手段对复合光催化剂的微观结构、形貌和性能进行了表征,并研究了可见光下降解亚甲基蓝(MB)的催化性能.研究表明,Ag@AgCl 的粒径约为20 nm,均匀分散在二维层状结构K4 Nb6 O17的表面上;贵金属Ag纳米粒子的表面等离子体效应显著,对可见光的吸收范围从400 nm 拓展到800 nm;Ag@AgCl(25%(质量分数))/K4Nb6O17在可见光照射60 min对亚甲基蓝的降解率为88.2%,远远高于单体Ag@AgCl和K4 Nb6 O17的活性.循环实验证明催化剂具有较好的稳定性,同时对苯酚和罗丹明 B(RhB)也具有一定的催化降解活性.淬灭实验表明自由基和空穴均为活性物种,并在此基础上提出降解机理.%The composite photocatalyst of Ag@AgCl/K4 Nb6 O17 was composed of by the oil-water self-assembly method,on the basis that K4 Nb6 O17 was prepared in the high-temperature solid-state method.The microstruc-ture,morphology and properties of Ag@AgCl/K4 Nb6 O17 composite photocatalyst were characterized by means of XRD,SEM,TEM,EDX,UV-vis,PL,BET;and the photocatalytic performance was valued by the degra-dation of methylene blue (MB),phenol,and Rhodamine B (RhB)under visible light irradiation.The results shows that Ag@AgCl particles with the size of about 20 nm were dispersed in two-dimensional layer structure on the surface of K4 Nb6 O17 and the absorption of visible light of the composite photocatalyst ranged from 400 to 800 nm due to the surface plasmon resonance of Ag.The degradation rate of MB solution over Ag@AgCl (25wt%)/K4 Nb6 O17 under the visible light irradiation reaches 88.2% in an hour,which is more than two times compared with the sum of the Ag@AgCl and K4 Nb6 O17 degradation rate.The composite photocatalyst still ex-hibited high catalytic activity after recycled five times

  5. Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin

    Directory of Open Access Journals (Sweden)

    De Rosa Mario

    2010-05-01

    Full Text Available Abstract Background Chondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as a human anti-inflammatory drug. Among bacteria, Escherichia coli K4 produces a capsule containing a non-sulphate chondroitin and its development may provide an efficient and cheap fermentative production of the polysaccharide. Results A random N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis was performed on E. coli K4 to isolate mutants showing an increased production of chondroitin. Several mutants were isolated, one of which, here named VZ15, produced about 80% more chondroitin than the wild type E. coli. We found that the mutant has a missense mutation in the codon 313 of kfoC, the gene encoding chondroitin polymerase (K4CP, with a change from arginine to glutamine. A docking analysis to explain the increased productivity of the K4CP enzyme is presented. Conclusion The enhanced chondroitin production by the E. coli K4 mutant reported here shows the validity of the strain improvement strategy for more cost-friendly fermentative processes in the production of this pharmaceutically important but so-far expensive polysaccharide.

  6. Common Core Literacy Lesson Plans: Ready-to-Use Resources, K-5

    Science.gov (United States)

    Davis, Lauren

    2013-01-01

    Schools nationwide are transitioning to the Common Core--our advice to you: Don't go it alone! Our new book, "Common Core Literacy Lesson Plans: Ready-to-Use Resources, K-5," shows you that teaching the Common Core State Standards in the elementary grades doesn't have to be intimidating! This easy-to-use guide provides model lesson plans for…

  7. Interaction of Escherichia coli K1 and K5 with Acanthamoeba castellanii Trophozoites and Cysts

    OpenAIRE

    Matin, Abdul; Jung, Suk-Yul

    2011-01-01

    The existence of symbiotic relationships between Acanthamoeba and a variety of bacteria is well-documented. However, the ability of Acanthamoeba interacting with host bacterial pathogens has gained particular attention. Here, to understand the interactions of Escherichia coli K1 and E. coli K5 strains with Acanthamoeba castellanii trophozoites and cysts, association assay, invasion assay, survival assay, and the measurement of bacterial numbers from cysts were performed, and nonpathogenic E. ...

  8. Pentapotassium praseodymium(III dilithium decafluoride, K5PrLi2F10

    Directory of Open Access Journals (Sweden)

    Anna Gagor

    2009-11-01

    Full Text Available The crystal structure of K5PrLi2F10 is isotypic with those of other K5RELi2F10 compounds (RE = Eu, Nd. The lanthanoid ions are isolated in K5PrLi2F10, with a mean separation between the Pr ions of 7.356 Å. It classifies this crystal as a so-called self-activated material containing lanthanoid ions within the matrix. Except for two K+ and two F− ions, all atoms are located on sites with m symmetry. In the structure, distorted PrF8 dodecahedra and two different LiF4 tetrahedra share F atoms, forming sheets parallel to (100. The isolated PrF8 dodecahedra exhibit a mean Pr—F distance of 2.406 Å. The K+ cations are located within and between these sheets, leading to highly irregular KFx polyhedra with coordination numbers of eight and nine for the alkali metal cations.

  9. One of the 10 cells of AA Injection Kicker K4

    CERN Multimedia

    1979-01-01

    The 3.5 GeV/c Antiproton Accumulator (AA) had 2 delay-line type injection kickers, K3 (12 cells) and K4 (10 cells). Here we see one of the K4 cells, with ferrite between stainless-steel plates. Pulse voltage: 61 kV; rise/fall-time 86 ns; flat-top 460 ns; top flatness +-2%. During injection, the open side of the C-shaped kickers was closed off with a fast shutter, so that their stray field would not perturb the stack of already accumulated antiprotons.

  10. DUOX2 promotes the elimination of the Klebsiella pneumoniae strain K5 from T24 cells through the reactive oxygen species pathway.

    Science.gov (United States)

    Lu, Huixia; Wu, Qi; Yang, Huijun

    2015-08-01

    Dual oxidase 2 (DUOX2) plays a major role in host defense in intestinal and airway epithelial cells through the reactive oxygen species (ROS) pathway. Klebsiella pneumoniae is a uropathogen that causes urinary tract infections. It is not known whether DUOX2 plays a role in host defense in bladder cancer epithelial cells. It is also not known whether Klebsiella pneumoniae invades T24 human bladder carcinoma cells and whether DUOX2 plays a role in eliminating the Klebsiella pneumoniae strain K5 through the ROS pathway in T24 cells. Thus, in the present study, we aimed to investigate the infectious capability of the Klebsiella pneumoniae K5 strain and the immunity-promoting capability of DUOX2 in T24 cells. We quantified the number of viable intracellular bacteria using the plate count method. DUOX2 expression was evaluated by western blot analysis and reverse transcription-quantitative PCR (RT-qPCR) following treatment with or without multiple cytokines, phorbol 12-myristate 13-acetate (PMA), muramyl dipeptide (MDP), N-acetylmuramyl-D-alanyl-D-isoglutamine (MDP-DD), H2O2 inhibitor, catalase (CAT), the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH) oxidase inhibitor, diphenyleneiodonium (DPI), or siRNA targeting DUOX2 (siDUOX2). The levels of ROS in the T24 cells infected with the K5 strain were examined following treatment with DPI, CAT or siDUOX2. Our results revealed that DUOX2 expression increased and the number of viable intracellular bacteria decreased in the T24 cells following infection with the K4 bacteria. Treatment with the cytokines and MDP and PMA also induced DUOX2 expression and decreased the number of viable intracellular bacteria. The levels of ROS also increased following treatment with the cytokines and MDP and PMA. However, when the cells were treated with the inhibitors (DPI or CAT), these effects were all reversed. Our data demonstrated that DUOX2 played an important role in innate immunity against bacterial cytoinvasion through the

  11. H3K4 demethylase activities repress proliferative and postmitotic aging

    Science.gov (United States)

    Alvares, Stacy M; Mayberry, Gaea A; Joyner, Ebony Y; Lakowski, Bernard; Ahmed, Shawn

    2014-01-01

    Homeostasis of postmitotic and proliferating cells is maintained by pathways that repress stress. We found that the Caenorhabditis elegans histone 3 lysine 4 (H3K4) demethylases RBR-2 and SPR-5 promoted postmitotic longevity of stress-resistant daf-2 adults, altered pools of methylated H3K4, and promoted silencing of some daf-2 target genes. In addition, RBR-2 and SPR-5 were required for germ cell immortality at a high temperature. Transgenerational proliferative aging was enhanced for spr-5; rbr-2 double mutants, suggesting that these histone demethylases may function sequentially to promote germ cell immortality by targeting distinct H3K4 methyl marks. RBR-2 did not play a comparable role in the maintenance of quiescent germ cells in dauer larvae, implying that it represses stress that occurs as a consequence of germ cell proliferation, rather than stress that accumulates in nondividing cells. We propose that H3K4 demethylase activities promote the maintenance of chromatin states during stressful growth conditions, thereby repressing postmitotic aging of somatic cells as well as proliferative aging of germ cells. PMID:24134677

  12. Purification of chondroitin precursor from Escherichia coli K4 fermentation broth using membrane processing.

    Science.gov (United States)

    Schiraldi, Chiara; Carcarino, Immacolata Loredana; Alfano, Alberto; Restaino, Odile Francesca; Panariello, Andrea; De Rosa, Mario

    2011-04-01

    Recently the possibility of producing the capsular polysaccharide K4, a fructosylated chondroitin, in fed-batch experiments was assessed. In the present study, a novel downstream process to obtain chondroitin from Escherichia coli K4 fermentation broth was developed. The process is simple, scalable and economical. In particular, downstream procedures were optimized with a particular aim of purifying a product suitable for further chemical modifications, in an attempt to develop a biotechnological platform for chondroitin sulfate production. During process development, membrane devices (ultrafiltration/diafiltration) were exploited, selecting the right cassette cut-offs for different phases of purification. The operational conditions (cross-flow rate and transmembrane pressure) used for the process were determined on an ÄKTA cross-flow instrument (GE Healthcare, USA), a lab-scale automatic tangential flow filtration system. In addition, parameters such as selectivity and throughput were calculated based on the analytical quantification of K4 and defructosylated K4, as well as the major contaminants. The complete downstream procedure yielded about 75% chondroitin with a purity higher than 90%.

  13. Research-Based Methods of Reading Instruction for English Language Learners, Grades K-4

    Science.gov (United States)

    Vaughn, Sharon; Linan-Thompson, Sylvia

    2007-01-01

    At last, a truly helpful, practical guide for K-4 teachers who want to ensure that their approach to teaching young English language learners is based on research and grounded in proven classroom practices. The authors of ASCD's best-selling book "Research-Based Methods of Reading Instruction, Grades K-3" explain how you can help the English…

  14. Research-Based Methods of Reading Instruction for English Language Learners, Grades K-4

    Science.gov (United States)

    Vaughn, Sharon; Linan-Thompson, Sylvia

    2007-01-01

    At last, a truly helpful, practical guide for K-4 teachers who want to ensure that their approach to teaching young English language learners is based on research and grounded in proven classroom practices. The authors of ASCD's best-selling book "Research-Based Methods of Reading Instruction, Grades K-3" explain how you can help the English…

  15. From Inception to Reflection: Ohio's K-4 Content-Enriched Mandarin Chinese Curriculum

    Science.gov (United States)

    Robinson, Deborah W.

    2009-01-01

    In 2006 the Ohio Department of Education (ODE) submitted and received a three-year Foreign Language Assistance Program grant from the U.S. Department of Education to write and pilot a K-4 content-enriched Mandarin curriculum and to build online professional development modules to support the curriculum. Once funded, ODE formed an advisory…

  16. Targeted deletion of Atg5 reveals differential roles of autophagy in keratin K5-expressing epithelia

    Energy Technology Data Exchange (ETDEWEB)

    Sukseree, Supawadee [Research Division of Biology and Pathobiology of the Skin, Department of Dermatology, Medical University of Vienna, Vienna (Austria); Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok (Thailand); Rossiter, Heidemarie; Mildner, Michael [Research Division of Biology and Pathobiology of the Skin, Department of Dermatology, Medical University of Vienna, Vienna (Austria); Pammer, Johannes [Institute of Clinical Pathology, Medical University of Vienna, Vienna (Austria); Buchberger, Maria; Gruber, Florian [Research Division of Biology and Pathobiology of the Skin, Department of Dermatology, Medical University of Vienna, Vienna (Austria); Watanapokasin, Ramida [Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok (Thailand); Tschachler, Erwin [Research Division of Biology and Pathobiology of the Skin, Department of Dermatology, Medical University of Vienna, Vienna (Austria); Eckhart, Leopold, E-mail: leopold.eckhart@meduniwien.ac.at [Research Division of Biology and Pathobiology of the Skin, Department of Dermatology, Medical University of Vienna, Vienna (Austria)

    2013-01-11

    Highlights: Black-Right-Pointing-Pointer We generated mice lacking Atg5 and autophagy in keratin K5-positive epithelia. Black-Right-Pointing-Pointer Suppression of autophagy in thymic epithelium was not associated with signs of autoimmunity. Black-Right-Pointing-Pointer Autophagy was required for normal terminal differentiation of preputial gland cells. Black-Right-Pointing-Pointer Autophagy-deficient cells of the preputial glands degraded nuclear DNA prematurely. -- Abstract: Autophagy contributes to the homeostasis of many tissues, yet its role in epithelia is incompletely understood. A recent report proposed that Atg5-dependent autophagy in thymic epithelial cells is essential for their function in the negative selection of self-reactive T-cells and, thus, for the suppression of tissue inflammation. Here we crossed mice carrying floxed alleles of the Atg5 gene with mice expressing the Cre recombinase under the control of the keratin K5 promoter to suppress autophagy in all K5-positive epithelia. The efficiency of autophagy abrogation was confirmed by immunoanalyses of LC3, which was converted to the autophagy-associated LC3-II form in normal but not Atg5-deficient cells, and of p62, which accumulated in Atg5-deficient cells. Mice carrying the epithelium-specific deletion of Atg5 showed normal weight gain, absence of tissue inflammation, and a normal morphology of the thymic epithelium. By contrast, autophagy-deficient epithelial cells of the preputial gland showed aberrant eosinophilic staining in histology and premature degradation of nuclear DNA during terminal differentiation. Taken together, the results of this study suggest that autophagy is dispensable for the suppression of autoimmunity by thymic epithelial cells but essential for normal differentiation of the preputial gland in mice.

  17. Minimal (C4,K4;7)-P-graphs%极小(C4,K4;7)-平面图

    Institute of Scientific and Technical Information of China (English)

    段春燕

    2014-01-01

    An (H1 ,H2;n)-P-graph is a graph with order n,which does not contain a subgraph isomorphic to H1 ,and its complement has no subgraph isomorphic to H2 .G is a minimal (H1 ,H2;n)-P-graph,if G is an (H1 ,H2;n)-P-graph and G′obtained by deleting any one edge from G is not an (H1 ,H2;n)-P-graph.In this paper,the minimal (C4 ,K4;7 )-P-graphs are given.%若平面图G没有与H1同构的子图,且G珔没有与H2同构的子图,则G称为(H1,H2)-平面图。(H1,H2;n)-平面图表示n阶的(H1,H2)-平面图。若G是(H1,H2;n)-平面图,去掉G的任意一条边,都不再是(H1,H2;n)-平面图,则G称为极小(H1,H2;n)-平面图。本文给出了所有的极小(C4,K4;7)-平面图,有助于证明PR(C4,K8)。

  18. AMPA, NMDA and kainate glutamate receptor subunits are expressed in human peripheral blood mononuclear cells (PBMCs) where the expression of GluK4 is altered by pregnancy and GluN2D by depression in pregnant women.

    Science.gov (United States)

    Bhandage, Amol K; Jin, Zhe; Hellgren, Charlotte; Korol, Sergiy V; Nowak, Krzysztof; Williamsson, Louise; Sundström-Poromaa, Inger; Birnir, Bryndis

    2017-04-15

    The amino acid glutamate opens cation permeable ion channels, the iGlu receptors. These ion channels are abundantly expressed in the mammalian brain where glutamate is the main excitatory neurotransmitter. The neurotransmitters and their receptors are being increasingly detected in the cells of immune system. Here we examined the expression of the 18 known subunits of the iGlu receptors families; α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), kainate, N-methyl-d-aspartate (NMDA) and delta in human peripheral blood mononuclear cells (PBMCs). We compared the expression of the subunits between four groups: men, non-pregnant women, healthy pregnant women and depressed pregnant women. Out of 18 subunits of the iGlu receptors, mRNAs for 11 subunits were detected in PBMCs from men and non-pregnant women; AMPA: GluA3, GluA4, kainate: GluK2, GluK4, GluK5, NMDA: GluN1, GluN2C, GluN2D, GluN3A, GluN3B, and delta: GluD1. In the healthy and the depressed pregnant women, in addition, the delta GluD2 subunit was identified. The mRNAs for GluK4, GluK5, GluN2C and GluN2D were expressed at a higher level than other subunits. Gender, pregnancy or depression during pregnancy altered the expression of GluA3, GluK4, GluN2D, GluN3B and GluD1 iGlu subunit mRNAs. The greatest changes recorded were the lower GluA3 and GluK4 mRNA levels in pregnant women and the higher GluN2D mRNA level in healthy but not in depressed pregnant women as compared to non-pregnant individuals. Using subunit specific antibodies, the GluK4, GluK5, GluN1, GluN2C and GluN2D subunit proteins were identified in the PBMCs. The results show expression of specific iGlu receptor subunit in the PBMCs and support the idea of physiology-driven changes of iGlu receptors subtypes in the immune cells. Copyright © 2017 Elsevier B.V. All rights reserved.

  19. Electrochemical Catalysis of Inorganic Complex K4[Fe(CN)6] by Shewanella oneidensis MR-1

    DEFF Research Database (Denmark)

    Zheng, Zhiyong; Wu, Ranran; Xiao, Yong

    to the redox proteins localized to the outer-membrane, for example, the MtrC, MtrB,MtrA and CymA2. Here we investigate its electrochemical properties towards redox inorganic redox compounds. It shows strong electrocatalysis toward electrochemical oxidation of K4[Fe(CN)6]. As a redox molecule, K4[Fe(CN)6] gives...... on the selectivity and electrocatalysis mechanisms of Shewanella oneidensis MR-1 are under investigation. The ability of Shewanella oneidensis MR-1 to catalyze redox action of inorganic metal complex compounds will provide an insight on metal cycles in nature....... disappearance of the cathodic peak and strengthen of the anodic peak, which is a typical catalysis feature of electrochemical oxidation. Further experiments show that Shewanella oneidensis MR-1 does not give such electrocatalysis to redox compounds such as Ru[(NH3)6]Cl3 and Resorufin. Systematic study...

  20. Timing of transcriptional quiescence during gametogenesis is controlled by global histone H3K4 demethylation.

    Science.gov (United States)

    Xu, Mengshu; Soloveychik, Maria; Ranger, Mathieu; Schertzberg, Michael; Shah, Zarna; Raisner, Ryan; Venkatasubrahmanyan, Shivkumar; Tsui, Kyle; Gebbia, Marinella; Hughes, Tim; van Bakel, Harm; Nislow, Corey; Madhani, Hiten D; Meneghini, Marc D

    2012-11-13

    Gametes are among the most highly specialized cells produced during development. Although gametogenesis culminates in transcriptional quiescence in plants and animals, regulatory mechanisms controlling this are unknown. Here, we confirm that gamete differentiation in the single-celled yeast Saccharomyces cerevisiae is accompanied by global transcriptional shutoff following the completion of meiosis. We show that Jhd2, a highly conserved JARID1-family histone H3K4 demethylase, activates protein-coding gene transcription in opposition to this programmed transcriptional shutoff, sustaining the period of productive transcription during spore differentiation. Moreover, using genome-wide nucleosome, H3K4me, and transcript mapping experiments, we demonstrate that JHD2 globally represses intergenic noncoding transcription during this period. The widespread transcriptional defects of JHD2 mutants are associated with precocious differentiation and the production of stress-sensitive spores, demonstrating that Jhd2 regulation of the global postmeiotic transcriptional program is critical for the production of healthy meiotic progeny.

  1. 不再当大男孩儿 KIA K4

    Institute of Scientific and Technical Information of China (English)

    张伟平

    2014-01-01

    东风悦达·起亚K系家族给我留下的印象,就好比是一群大男孩儿,各个都拥有一副帅气的容貌且充满活力。不过从今天开始,K系家族出现了一名"另类",一款相对成熟、稳重的车型——K4!

  2. Supplement to CCM.D-K4 'Hydrometer' report: linkage of EURAMET.M.D-K4 comparison, SIM.M.D-K4 comparison and the supplementary SIM.M.D-S2 to CCM.D-K4 'Hydrometer'

    Science.gov (United States)

    Lorefice, S.; Becerra, L. O.

    2017-01-01

    Evaluation of different types of comparisons to a common set of reference values of a CIPM key comparison is essential to satisfy the concept of the CIPM Mutual Recognition Arrangement (CIPM MRA), where the DoEs of any participant who took part in comparisons should be within the Calibration and Measurement Capability (CMC) section of the CIPM MRA Key Comparison Data Base. The subject of this supplementary report is therefore to present the equivalence of each National Metrological Institute (NMI) participant in the CCM.D-K4 'Hydrometer' key comparison, which was performed in the density range 600 kg/m3 to 2000 kg/m3 at the temperature of 20 °C, and the linkage of the European and Inter-American NMI results performed in the RMO.M.D-K4 comparisons as well as those of the supplementary SIM.M.D-S2 to the common set of KCRVs of the CCM.D-K4 'Hydrometer'. The linking procedure has been obtained by numerical simulation, based on the Monte Carlo method, in which the differences in the results of the different comparison between the intended laboratory and one or more linking laboratory/ies, which took part in both comparisons, are correlated with a continuous function describing the DoEs of the linking laboratory/ies with respect to the common set of KCRVs of the CCM.D-K4. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  3. Drosophila KDM2 is a H3K4me3 demethylase regulating nucleolar organization

    Directory of Open Access Journals (Sweden)

    Birchler James A

    2009-10-01

    Full Text Available Abstract Background CG11033 (dKDM2 is the Drosophila homolog of the gene KDM2B. dKDM2 has been known to possess histone lysine demethylase activity towards H3K36me2 in cell lines and it regulates H2A ubiquitination. The human homolog of the gene has dual activity towards H3K36me2 as well as H3K4me3, and plays an important role in cellular senescence. Findings We have used transgenic flies bearing an RNAi construct for the dKDM2 gene. The knockdown of dKDM2 gene was performed by crossing UAS-RNAi-dKDM2 flies with actin-Gal4 flies. Western blots of acid extracted histones and immunofluoresence analysis of polytene chromosome showed the activity of the enzyme dKDM2 to be specific for H3K4me3 in adult flies. Immunofluoresence analysis of polytene chromosome also revealed the presence of multiple nucleoli in RNAi knockdown mutants of dKDM2 and decreased H3-acetylation marks associated with active transcription. Conclusion Our findings indicate that dKDM2 is a histone lysine demethylase with specificity for H3K4me3 and regulates nucleolar organization.

  4. Inducible Deletion of Protein Kinase Map4k4 in Obese Mice Improves Insulin Sensitivity in Liver and Adipose Tissues.

    Science.gov (United States)

    Danai, Laura V; Flach, Rachel J Roth; Virbasius, Joseph V; Menendez, Lorena Garcia; Jung, Dae Young; Kim, Jong Hun; Kim, Jason K; Czech, Michael P

    2015-07-01

    Studies in vitro suggest that mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4) attenuates insulin signaling, but confirmation in vivo is lacking since Map4k4 knockout is lethal during embryogenesis. We thus generated mice with floxed Map4k4 alleles and a tamoxifen-inducible Cre/ERT2 recombinase under the control of the ubiquitin C promoter to induce whole-body Map4k4 deletion after these animals reached maturity. Tamoxifen administration to these mice induced Map4k4 deletion in all tissues examined, causing decreased fasting blood glucose concentrations and enhanced insulin signaling to AKT in adipose tissue and liver but not in skeletal muscle. Surprisingly, however, mice generated with a conditional Map4k4 deletion in adiponectin-positive adipocytes or in albumin-positive hepatocytes displayed no detectable metabolic phenotypes. Instead, mice with Map4k4 deleted in Myf5-positive tissues, including all skeletal muscles tested, were protected from obesity-induced glucose intolerance and insulin resistance. Remarkably, these mice also showed increased insulin sensitivity in adipose tissue but not skeletal muscle, similar to the metabolic phenotypes observed in inducible whole-body knockout mice. Taken together, these results indicate that (i) Map4k4 controls a pathway in Myf5-positive cells that suppresses whole-body insulin sensitivity and (ii) Map4k4 is a potential therapeutic target for improving glucose tolerance and insulin sensitivity in type 2 diabetes.

  5. Genomic distribution of CHD7 on chromatin tracks H3K4 methylation patterns.

    Science.gov (United States)

    Schnetz, Michael P; Bartels, Cynthia F; Shastri, Kuntal; Balasubramanian, Dheepa; Zentner, Gabriel E; Balaji, Ravishankar; Zhang, Xiaodong; Song, Lingyun; Wang, Zhenghe; Laframboise, Thomas; Crawford, Gregory E; Scacheri, Peter C

    2009-04-01

    CHD7 is a member of the chromodomain helicase DNA binding domain family of ATP-dependent chromatin remodeling enzymes. De novo mutation of the CHD7 gene is a major cause of CHARGE syndrome, a genetic disease characterized by a complex constellation of birth defects (Coloboma of the eye, Heart defects, Atresia of the choanae, severe Retardation of growth and development, Genital abnormalities, and Ear abnormalities). To gain insight into the function of CHD7, we mapped the distribution of the CHD7 protein on chromatin using the approach of chromatin immunoprecipitation on tiled microarrays (ChIP-chip). These studies were performed in human colorectal carcinoma cells, human neuroblastoma cells, and mouse embryonic stem (ES) cells before and after differentiation into neural precursor cells. The results indicate that CHD7 localizes to discrete locations along chromatin that are specific to each cell type, and that the cell-specific binding of CHD7 correlates with a subset of histone H3 methylated at lysine 4 (H3K4me). The CHD7 sites change concomitantly with H3K4me patterns during ES cell differentiation, suggesting that H3K4me is part of the epigenetic signature that defines lineage-specific association of CHD7 with specific sites on chromatin. Furthermore, the CHD7 sites are predominantly located distal to transcription start sites, most often contained within DNase hypersensitive sites, frequently conserved, and near genes expressed at relatively high levels. These features are similar to those of gene enhancer elements, raising the possibility that CHD7 functions in enhancer mediated transcription, and that the congenital anomalies in CHARGE syndrome are due to alterations in transcription of tissue-specific genes normally regulated by CHD7 during development.

  6. Synthesis and characterization of the ternary telluroargentate K4[Ag18Te11

    KAUST Repository

    Davaasuren, Bambar

    2014-10-19

    The ternary potassium telluroargentate(I), K4[Ag18Te11], was prepared by solvothermal synthesis in ethylenediamine at 160 °C. It crystallizes in the cubic space group Fm3¯ m (no. 225) with the cell parameter a = 18.6589(6) Å. The crystal structure can be described as a [Ag18Te11]4- three-dimensional anionic framework with the voids accommodating potassium cations. Chemical bonding analysis reveals polar covalent Ag-Te bonds and considerable Ag-Ag interactions, which support the complex anionic character of the structure. The compound is thermally stable up to 450 °C in an inert atmosphere.

  7. Structural insight into the recognition of the H3K4me3 mark by the TFIID subunit TAF3

    NARCIS (Netherlands)

    van Ingen, H.|info:eu-repo/dai/nl/297054651; van Schaik, F.M.A.; Wienk, H.|info:eu-repo/dai/nl/203884884; Ballering, J.|info:eu-repo/dai/nl/325784876; Rehmann, H.; Dechesne, A.C.|info:eu-repo/dai/nl/304841684; Kruijzer, J.A.W.; Liskamp, R.M.J.|info:eu-repo/dai/nl/069091315; Timmers, H.T.M.; Boelens, R.|info:eu-repo/dai/nl/070151407

    2008-01-01

    Trimethylation of lysine residue K4 of histone H3 (H3K4me3) strongly correlates with active promoters for RNA polymerase II-transcribed genes. Several reader proteins, including the basal transcription factor TFIID, for this nucleosomal mark have been identified. Its TAF3 subunit specifically binds

  8. Engineering Computer Games: A Parallel Learning Opportunity for Undergraduate Engineering and Primary (K-5 Students

    Directory of Open Access Journals (Sweden)

    Mark Michael Budnik

    2011-04-01

    Full Text Available In this paper, we present how our College of Engineering is developing a growing portfolio of engineering computer games as a parallel learning opportunity for undergraduate engineering and primary (grade K-5 students. Around the world, many schools provide secondary students (grade 6-12 with opportunities to pursue pre-engineering classes. However, by the time students reach this age, many of them have already determined their educational goals and preferred careers. Our College of Engineering is developing resources to provide primary students, still in their educational formative years, with opportunities to learn more about engineering. One of these resources is a library of engineering games targeted to the primary student population. The games are designed by sophomore students in our College of Engineering. During their Introduction to Computational Techniques course, the students use the LabVIEW environment to develop the games. This software provides a wealth of design resources for the novice programmer; using it to develop the games strengthens the undergraduates

  9. Electrochemical Catalysis of Inorganic Complex K4[Fe(CN)6] by Shewanellaoneidensis MR-1

    DEFF Research Database (Denmark)

    Zheng, Zhiyong; Wu, Ranran; Xiao, Yong

    The interaction between metal and bacteria is a universal and important biogeochemical processin environment. As a dissimilatory metal reduction bacterium, the electrochemically activebacteriium Shewanella oneidensis MR-1 can transfer intracellular electrons to minerals. This ability is attribute...... andelectrocatalysis mechanisms of S. oneidensis MR-1 are under investigation. The ability of S. oneidensis MR-1 to catalyze redoxaction of inorganic metal complex compounds will provide an insight on metal cycles in nature....... to the redox proteins localized on the outer-membrane, for example, the MtrC, MtrB, MtrA and CymA2. Here we investigate its electrochemical properties towards redoxinorganic redox compounds. It shows strong electrocatalysis toward electrochemical oxidation of K4[Fe(CN)6]. As a redox molecule, K4[Fe(CN)6] gives......, with almost disappearance of the cathodic peak and strengthen of the anodic peak, which is a typical catalysis feature of electrochemical oxidation. Further experiments show that S. oneidensis MR-1 does not give such electrocatalysis toredox compounds such as Ru[(NH3)6]Cl3and Resorufin. Selectivity...

  10. An unexpected oxidation: NaK5Cl2(S2O6)2 revisited

    Science.gov (United States)

    Harrison, William T. A.; Plater, M. John

    2017-01-01

    The title compound, NaK5Cl2(S2O6)2 [systematic name: sodium penta­potassium dichloride bis­(di­thio­nate)], arose as an unexpected product from an organic synthesis that used di­thio­nite (S2O4 2−) ions as a reducing agent to destroy excess permanganate ions. Compared to the previous study [Stanley (1953 ▸). Acta Cryst. 6, 187–196], the present tetra­gonal structure exhibits a root 2a × root 2a × c super-cell due to subtle changes in the orientations of the di­thio­nate anions. The structure can be visualized as a three-dimensional framework of [001] columns of alternating trans-NaO4Cl2 and KO4Cl2 octa­hedra cross-linked by the di­thio­nate ions with the inter­stices occupied by KO6Cl2 polyhedra to generate a densely packed three-dimensional framework. The asymmetric unit comprises two sodium ions (site symmetries 4 and -4, four potassium ions (site symmetries = -4, 4, 1 and 1), three chloride ions (site symmetries = 4, 4 and 2) and two half-di­thio­nate ions (all atoms on general positions). Both di­thio­nate ions are completed by crystallographic inversion symmetry. The crystal chosen for data collection was found to be rotationally twinned by 180° about the [100] axis in reciprocal space with a 0.6298 (13):0.3702 (13) domain ratio. PMID:28217339

  11. Factors affecting early elementary (K--4) teachers' introduction of the nature of science: A national survey

    Science.gov (United States)

    Sweeney, Sophia Jean

    A researcher-developed questionnaire regarding the importance and developmental appropriateness of 12 specific elements of the nature of science (Alshamrani, 2008) for early elementary (kindergarten through fourth grade [K-4]) science instruction was mailed to a random sample of U.S. K-4 teachers. At least half (N = 377) of the respondents reported introducing the inferential, empirical, creative, collaborative, tentative, and cultural aspects of the nature of science (NOS) during the current school year, as well as the idea that no single step-by-step scientific method exists. Over 90% of respondents identified the inferential, empirical, and creative aspects of the NOS as developmentally appropriate for the grade level taught. Based on a 5 point scale (0=not at all important and 4 =very important), the mean scores of eight NOS elements were above a value of three, which corresponded with the descriptor somewhat important to include in K-4 science instruction: the inferential, empirical, creative, collaborative, cultural, and tentative NOS, along with the ideas that replication is an important aspect of experimental research and that no single stepwise scientific method exists. A series of binary logistic regression analyses were used to explore the impact of three predictor variables (developmental appropriateness, importance, and presence in state standards) on teachers' self-reported introduction of each of the NOS elements during the 2009-2010 school year. The data for the presence of the NOS elements in state standards were extracted from a previous study (McComas, Lee, & Sweeney, 2009). Developmental appropriateness was a significant predictor of teachers' introduction of the NOS element for all except the collaborative, empirical and inferential aspects of NOS. Importance was a significant predictor for all 12 NOS elements of interest. A NOS element's presence in the state standards significantly predicted teachers' introduction of that NOS element for only

  12. Analysis of the Mitogen-activated protein kinase kinase 4 (MAP2K4 tumor suppressor gene in ovarian cancer

    Directory of Open Access Journals (Sweden)

    Ryland Georgina L

    2011-05-01

    Full Text Available Abstract Background MAP2K4 is a putative tumor and metastasis suppressor gene frequently found to be deleted in various cancer types. We aimed to conduct a comprehensive analysis of this gene to assess its involvement in ovarian cancer. Methods We screened for mutations in MAP2K4 using High Resolution Melt analysis of 149 primary ovarian tumors and methylation at the promoter using Methylation-Specific Single-Stranded Conformation Polymorphism analysis of 39 tumors. We also considered the clinical impact of changes in MAP2K4 using publicly available expression and copy number array data. Finally, we used siRNA to measure the effect of reducing MAP2K4 expression in cell lines. Results In addition to 4 previously detected homozygous deletions, we identified a homozygous 16 bp truncating deletion and a heterozygous 4 bp deletion, each in one ovarian tumor. No promoter methylation was detected. The frequency of MAP2K4 homozygous inactivation was 5.6% overall, and 9.8% in high-grade serous cases. Hemizygous deletion of MAP2K4 was observed in 38% of samples. There were significant correlations of copy number and expression in three microarray data sets. There was a significant correlation between MAP2K4 expression and overall survival in one expression array data set, but this was not confirmed in an independent set. Treatment of JAM and HOSE6.3 cell lines with MAP2K4 siRNA showed some reduction in proliferation. Conclusions MAP2K4 is targeted by genetic inactivation in ovarian cancer and restricted to high grade serous and endometrioid carcinomas in our cohort.

  13. 关于多重多部图λKn(t)的(K4-e,λ)—分解%Decomposition of Complete Multipartite Multi-graph λKn(t) into K4-e

    Institute of Scientific and Technical Information of China (English)

    赵彤

    2001-01-01

    Hoffman在文献[6]中已经完全解决了多重完全图λKn(t)的(K4-e,λ)一分解问题.本文将Hoffman的结果从多重完全图推广到多重多部图,证明了λKn(t)的(K4-e,λ)-分解存在的充分必要条件.

  14. Inhibition of H3K4me2 Demethylation Protects Auditory Hair Cells from Neomycin-Induced Apoptosis.

    Science.gov (United States)

    He, Yingzi; Yu, Huiqian; Cai, Chengfu; Sun, Shan; Chai, Renjie; Li, Huawei

    2015-08-01

    Aminoglycoside-induced hair cell loss is a major cause of hearing impairment in children and deserves more attention in medical research. Epigenetic mechanisms have been shown to protect hair cells from ototoxic drugs. In this study, we focused on the role of dimethylated histone H3K4 (H3K4me2) in hair cell survival. To investigate the effects of lysine-specific demethylase 1 (LSD1)--the histone demethylase primarily responsible for demethylating H3K4me2--on neomycin-induced hair cell loss, isolated cochleae were pretreated with LSD1 inhibitors followed by neomycin exposure. There was a severe loss of hair cells in the organ of Corti after neomycin exposure, and inhibition of LSD1 significantly protected against neomycin-induced hair cell loss. H3K4me2 expression in the nuclei of hair cells decreased after exposure to neomycin, and blocking the decreased expression of H3K4me2 with LSD1 inhibitors prevented hair cell loss. Local delivery of these inhibitors in vivo also protected hair cells from neomycin-induced ototoxicity and maintained the hearing threshold in mice as determined by auditory brain stem response. This inhibition of neomycin-induced apoptosis occurs via reduced caspase-3 activation. Together, our findings demonstrate the protective role for H3K4me2 against neomycin-induced hair cell loss and hearing loss.

  15. Albuquerque, NM, USA

    Science.gov (United States)

    1991-01-01

    Albuquerque, NM (35.0N, 106.5W) is situated on the edge of the Rio Grande River and flood plain which cuts across the image. The reddish brown surface of the Albuquerque Basin is a fault depression filled with ancient alluvial fan and lake bed sediments. On the slopes of the Manzano Mountains to the east of Albuquerque, juniper and other timber of the Cibola National Forest can be seen as contrasting dark tones of vegetation.

  16. Water in Comet 2/2003 K4 (LINEAR) with Spitzer

    CERN Document Server

    Woodward, Charles E; Bockelee-Morvan, Dominique; Gehrz, R D

    2007-01-01

    We present sensitive 5.5 to 7.6 micron spectra of comet C/2003 K4 (LINEAR) obtained on 16 July 2004 (r_{h} = 1.760 AU, Delta_{Spitzer} = 1.409 AU, phase angle 35.4 degrees) with the Spitzer Space Telescope. The nu_{2} vibrational band of water is detected with a high signal-to-noise ratio (> 50). Model fitting to the best spectrum yields a water ortho-to-para ratio of 2.47 +/- 0.27, which corresponds to a spin temperature of 28.5^{+6.5}_{-3.5} K. Spectra acquired at different offset positions show that the rotational temperature decreases with increasing distance from the nucleus, which is consistent with evolution from thermal to fluorescence equilibrium. The inferred water production rate is (2.43 +/- 0.25) \\times 10^{29} molec. s^{-1}. The spectra do not show any evidence for emission from PAHs and carbonate minerals, in contrast to results reported for comets 9P/Tempel 1 and C/1995 O1 (Hale-Bopp). However, residual emission is observed near 7.3 micron the origin of which remains unidentified.

  17. Improved immunoelectron microscopic method for localizing cytoskeletal proteins in Lowicryl K4M embedded tissues.

    Science.gov (United States)

    Loesser, K E; Doane, K J; Wilson, F J; Roisen, F J; Malamed, S

    1986-11-01

    We have modified the Lowicryl K4M low-temperature dehydration and embedding procedure for immunoelectron microscopy to provide improved ultrastructural detail and facilitate the localization of actin and tubulin in isolated rat adrenocortical cells, chick spinal cord with attached dorsal root ganglia (SC-DRG), and cultured dorsal root ganglia (DRG). Cells and tissues were fixed for immunocytochemistry either in a mixture of 2% paraformaldehyde and 0.25% glutaraldehyde (0.1 M PIPES buffer, pH 7.3) or in a mixture of 0.3% glutaraldehyde and 1.0% ethyldimethylaminopropylcarbodiimide (0.1 M phosphate buffered saline, pH 7.3). Dehydration was in ethanol at progressively lower temperatures to -35 degrees C. Infiltration at -35 degrees C was followed by ultraviolet polymerization at -20 degrees C. Comparable samples were fixed in glutaraldehyde and osmium tetroxide and embedded in Epon 812 or Epon-Araldite. Post-embedding immunostaining of thin sections utilized commercially available monoclonal antibodies to tubulin and actin followed by the protein A-gold technique (Roth et al., Endocrinology 108:247, 1981). Actin immunoreactivity was observed at the periphery of mitochondria and between mitochondria and lipid droplets in rat adrenocortical cells and at the periphery of neuronal cell processes of SC-DRG. Tubulin immunoreactivity was associated with microtubules throughout neurites of cultured DRG. Our modified technique allows preservation of ultrastructural details as well as localization of antigens by immunoelectron microscopy.

  18. Inter-RMO Key Comparison EUROMET.L-K5.2004: Calibration of a step gauge

    DEFF Research Database (Denmark)

    Prieto, Emilio; Brown, Nicholas; Lassila, Antti

    2012-01-01

    The results of the inter-RMO key comparison EUROMET.L-K5.2004 on the calibration of a step gauge are reported. Eighteen National Metrology Institutes and one Designated Institute from four different metrological regions all over the world participated in this comparison which lasted three years...

  19. Moving the School and Dancing Education: Case Study Research of K-5 Students' Experiences in a Dance Residency

    Science.gov (United States)

    Leonard, Alison E.

    2012-01-01

    This dissertation chronicles the qualitative case study of a dance artist-in-residence at a diverse and inclusive K-5 school in an urban district, integrating science, social studies, physical education, music, and visual arts school curriculum and culminating in two public performances. This study focused on how students made meaning through this…

  20. The Role of H3K4me3 in Transcriptional Regulation Is Altered in Huntington's Disease.

    Directory of Open Access Journals (Sweden)

    Xianjun Dong

    Full Text Available Huntington's disease (HD is an autosomal-dominant neurodegenerative disorder resulting from expansion of CAG repeats in the Huntingtin (HTT gene. Previous studies have shown mutant HTT can alter expression of genes associated with dysregulated epigenetic modifications. One of the most widely studied chromatin modifications is trimethylated lysine 4 of histone 3 (H3K4me3. Here, we conducted the first comprehensive study of H3K4me3 ChIP-sequencing in neuronal chromatin from the prefrontal cortex of six HD cases and six non-neurologic controls, and its association with gene expression measured by RNA-sequencing. We detected 2,830 differentially enriched H3K4me3 peaks between HD and controls, with 55% of them down-regulated in HD. Although H3K4me3 signals are expected to be associated with mRNA levels, we found an unexpected discordance between altered H3K4me3 peaks and mRNA levels. Gene ontology (GO term enrichment analysis of the genes with differential H3K4me3 peaks, revealed statistically significantly enriched GO terms only in the genes with down-regulated signals in HD. The most frequently implicated biological process terms are organ morphogenesis and positive regulation of gene expression. More than 9,000 H3K4me3 peaks were located not near any recognized transcription start sites and approximately 36% of these "distal" peaks co-localized to known enhancer sites. Six transcription factors and chromatin remodelers are differentially enriched in HD H3K4me3 distal peaks, including EZH2 and SUZ12, two core subunits of the polycomb repressive complex 2 (PRC2. Moreover, PRC2 repressive state was significantly depleted in HD-enriched peaks, suggesting the epigenetic role of PRC2 inhibition associated with up-regulated H3K4me3 in Huntington's disease. In summary, our study provides new insights into transcriptional dysregulation of Huntington's disease by analyzing the differentiation of H3K4me3 enrichment.

  1. Comparative analyses of H3K4 and H3K27 trimethylations between the mouse cerebrum and testis.

    Science.gov (United States)

    Cui, Peng; Liu, Wanfei; Zhao, Yuhui; Lin, Qiang; Zhang, Daoyong; Ding, Feng; Xin, Chengqi; Zhang, Zhang; Song, Shuhui; Sun, Fanglin; Yu, Jun; Hu, Songnian

    2012-04-01

    The global features of H3K4 and H3K27 trimethylations (H3K4me3 and H3K27me3) have been well studied in recent years, but most of these studies were performed in mammalian cell lines. In this work, we generated the genome-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChIP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLiD technology. We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression, function and development. Moreover, we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes, which contradicts the findings in previous studies. Finally, we observed that bivalent domains, with both H3K4me3 and H3K27me3, existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes. However, the bivalent domains tend towards a "winner-takes-all" approach to regulate the expression of associated genes. We also verified the above results in mouse ES cells. As expected, the results in ES cells are consistent with those in cerebrum and testis. In conclusion, we present two very important findings. One is that H3K4me3 and H3K27me3 rarely occur in silent genes. The other is that bivalent domains may adopt a "winner-takes-all" principle to regulate gene expression. Copyright © 2012 Beijing Institute of Genomics, Chinese Academy of Sciences. Published by Elsevier Ltd. All rights reserved.

  2. Predicting the probability of H3K4me3 occupation at a base pair from the genome sequence context.

    Science.gov (United States)

    Ha, Misook; Hong, Soondo; Li, Wen-Hsiung

    2013-05-01

    Histone modifications regulate chromatin structure and gene expression. Although nucleosome formation is known to be affected by primary DNA sequence composition, no sequence signature has been identified for histone modifications. It is known that dense H3K4me3 nucleosome sites are accompanied by a low density of other nucleosomes and are associated with gene activation. This observation suggests a different sequence composition of H3K4me3 from other nucleosomes. To understand the relationship between genome sequence and chromatin structure, we studied DNA sequences at histone modification sites in various human cell types. We found sequence specificity for H3K4me3, but not for other histone modifications. Using the sequence specificities of H3 and H3K4me3 nucleosomes, we developed a model that computes the probability of H3K4me3 occupation at each base pair from the genome sequence context. A comparison of our predictions with experimental data suggests a high performance of our method, revealing a strong association between H3K4me3 and specific genomic DNA context. The high probability of H3K4me3 occupation occurs at transcription start and termination sites, exon boundaries and binding sites of transcription regulators involved in chromatin modification activities, including histone acetylases and enhancer- and insulator-associated factors. Thus, the human genome sequence contains signatures for chromatin modifications essential for gene regulation and development. Our method may be applied to find new sequence elements functioning by chromatin modulation. Software and supplementary data are available at Bioinformatics online.

  3. Comparative Analyses of H3K4 and H3K27 Trimethylations Between the Mouse Cerebrum and Testis

    KAUST Repository

    Cui, Peng

    2012-06-08

    The global features of H3K4 and H3K27 trimethylations (H3K4me3 and H3K27me3) have been well studied in recent years, but most of these studies were performed in mammalian cell lines. In this work, we generated the genome-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChIP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLiD technology. We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression, function and development. Moreover, we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes, which contradicts the findings in previous studies. Finally, we observed that bivalent domains, with both H3K4me3 and H3K27me3, existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes. However, the bivalent domains tend towards a “winner-takes-all” approach to regulate the expression of associated genes. We also verified the above results in mouse ES cells. As expected, the results in ES cells are consistent with those in cerebrum and testis. In conclusion, we present two very important findings. One is that H3K4me3 and H3K27me3 rarely occur in silent genes. The other is that bivalent domains may adopt a “winner-takes-all” principle to regulate gene expression.

  4. Predicting the probability of H3K4me3 occupation at a base pair from the genome sequence context

    Science.gov (United States)

    Ha, Misook; Hong, Soondo; Li, Wen-Hsiung

    2013-01-01

    Motivation: Histone modifications regulate chromatin structure and gene expression. Although nucleosome formation is known to be affected by primary DNA sequence composition, no sequence signature has been identified for histone modifications. It is known that dense H3K4me3 nucleosome sites are accompanied by a low density of other nucleosomes and are associated with gene activation. This observation suggests a different sequence composition of H3K4me3 from other nucleosomes. Approach: To understand the relationship between genome sequence and chromatin structure, we studied DNA sequences at histone modification sites in various human cell types. We found sequence specificity for H3K4me3, but not for other histone modifications. Using the sequence specificities of H3 and H3K4me3 nucleosomes, we developed a model that computes the probability of H3K4me3 occupation at each base pair from the genome sequence context. Results: A comparison of our predictions with experimental data suggests a high performance of our method, revealing a strong association between H3K4me3 and specific genomic DNA context. The high probability of H3K4me3 occupation occurs at transcription start and termination sites, exon boundaries and binding sites of transcription regulators involved in chromatin modification activities, including histone acetylases and enhancer- and insulator-associated factors. Thus, the human genome sequence contains signatures for chromatin modifications essential for gene regulation and development. Our method may be applied to find new sequence elements functioning by chromatin modulation. Availability: Software and supplementary data are available at Bioinformatics online. Contact: misook.ha@samsung.com or wli@uchicago.edu Supplementary information: Supplementary data are available at Bioinformatics online. PMID:23511541

  5. Effect of stearic acid on the properties of metronidazole/methocel K4M floating matrices

    Directory of Open Access Journals (Sweden)

    Belem Lara-Hernández

    2009-09-01

    Full Text Available The properties of metronidazole/Methocel K4M sustained release floating tablets have been studied varying the proportion of the lubricant, stearic acid, on formulations with and without sodium bicarbonate. The variables studied include technological properties of the tablets such as tablet hardness and ejection pressure, the drug release profile, the hydration kinetics and the floating behaviour. The presence of stearic acid and sodium bicarbonate improves the floating behaviour for more than 8 hours. The hydration volume, the tablet hardness and the ejection pressure decrease as the stearic acid content increases and the polymer content decreases. Drug dissolution increases with increasing proportions of stearic acid and decreasing proportions of the polymer in the tablets. The presence of sodium bicarbonate extends the differences in dissolution produced by stearic acid. These results are attributed to decreasing matrices coherence with an increasing quantity of stearic acid and a reducing polymer proportion. The carbon dioxide bubbles produced by sodium bicarbonate expand the matrices facilitating the dissolution, although their presence obstructs also the diffusion path through the hydrated gel layer.Estudaram-se as propriedades de comprimidos flutuantes de metronidazol/Methocel K4M de liberação controlada, variando-se a proporção do lubrificante, ácido esteárico, nas formulações com e sem bicarbonato de sódio. As variáveis estudadas incluem propriedades tecnológicas dos comprimidos, tais como dureza, pressão de ejeção, perfil de liberação do fármaco, cinética de hidratação e comportamento de flutuação. A presença de ácido esteárico e do bicarbonato de sódio melhora o comportamento de flutuação para mais de 8 horas. O volume de hidratação, a dureza e a pressão de ejeção do comprimido decrescem à medida que o conteúdo de ácido esteárico e de polímero diminui. A dissolução do fármaco aumenta com o

  6. Aberrant intracellular localization of H3k4me3 demonstrates an early epigenetic phenomenon in Alzheimer’s disease

    Science.gov (United States)

    Mastroeni, Diego; Delvaux, Elaine; Nolz, Jennifer; Tan, Yuyan; Grover, Andrew; Oddo, Salvatore; Coleman, Paul D.

    2016-01-01

    We have previously reported in Alzheimer’s disease (AD) the mislocalization of epigenetic molecules between the cell nucleus and the cytoplasm. We have extended our finding to include the aberrant localization of histone 3 trimethylation on lysine 4 (H3k4me3), an epigenetic mark associated with actively transcribing genes as well as those poised for transcription. These findings raise the question of where the ectopic localization of H3k4me3 fits within the cascade of cell biological events in the progression of AD. We, therefore, examined the expression and intracellular location of H3k4me3 as a function of Braak stage and also in relation to a series of tau markers that are indicative of disease state. Both lines of evidence showed that ectopic localization of H3k4me3 is early in the course of disease. Because of the known role of H3k4me3 in the expression of synaptic genes, our data suggest an epigenetic role in synaptic deficits early in the course of AD. PMID:26553823

  7. Aberrant intracellular localization of H3k4me3 demonstrates an early epigenetic phenomenon in Alzheimer's disease.

    Science.gov (United States)

    Mastroeni, Diego; Delvaux, Elaine; Nolz, Jennifer; Tan, Yuyan; Grover, Andrew; Oddo, Salvatore; Coleman, Paul D

    2015-12-01

    We have previously reported in Alzheimer's disease (AD) the mislocalization of epigenetic molecules between the cell nucleus and the cytoplasm. We have extended our finding to include the aberrant localization of histone 3 trimethylation on lysine 4 (H3k4me3), an epigenetic mark associated with actively transcribing genes as well as those poised for transcription. These findings raise the question of where the ectopic localization of H3k4me3 fits within the cascade of cell biological events in the progression of AD. We, therefore, examined the expression and intracellular location of H3k4me3 as a function of Braak stage and also in relation to a series of tau markers that are indicative of disease state. Both lines of evidence showed that ectopic localization of H3k4me3 is early in the course of disease. Because of the known role of H3k4me3 in the expression of synaptic genes, our data suggest an epigenetic role in synaptic deficits early in the course of AD. Copyright © 2015 Elsevier Inc. All rights reserved.

  8. Differential dynamics of histone H3 methylation at positions K4 and K9 in the mouse zygote

    Directory of Open Access Journals (Sweden)

    Walter Jörn

    2004-09-01

    Full Text Available Abstract Background In the mouse zygote the paternal genome undergoes dramatic structural and epigenetic changes. Chromosomes are decondensed, protamines replaced by histones and DNA is rapidly and actively demethylated. The epigenetic asymmetry between parental genomes remains at least until the 2-cell stage suggesting functional differences between paternal and maternal genomes during early cleavage stages. Results Here we analyzed the timing of histone deposition on the paternal pronucleus and the dynamics of histone H3 methylation (H3/K4mono-, H3/K4tri- and H3/K9di-methylation immediately after fertilization. Whereas maternal chromatin maintains all types of histone H3 methylation throughout the zygotic development, paternal chromosomes acquire new and unmodified histones shortly after fertilization. In the following hours we observe a gradual increase in H3/K4mono-methylation whereas H3/K4tri-methylation is not present before latest pronuclear stages. Histone H3/K9di-methylation is completely absent from the paternal pronucleus, including metaphase chromosomes of the first mitotic stage. Conclusion Parallel to the epigenetic asymmetry in DNA methylation, chromatin modifications are also different between both parental genomes in the very first hours post fertilization. Whereas methylation at H3/K4 gradually becomes similar between both genomes, H3/K9 methylation remains asymmetric.

  9. Association of MAP4K4 gene single nucleotide polymorphism with mastitis and milk traits in Chinese Holstein cattle.

    Science.gov (United States)

    Bhattarai, Dinesh; Chen, Xing; Ur Rehman, Zia; Hao, Xingjie; Ullah, Farman; Dad, Rahim; Talpur, Hira Sajjad; Kadariya, Ishwari; Cui, Lu; Fan, Mingxia; Zhang, Shujun

    2017-02-01

    The objective of the studies presented in this Research Communication was to investigate the association of single nucleotide polymorphisms present in the MAP4K4 gene with different milk traits in dairy cows. Based on previous QTL fine mapping results on bovine chromosome 11, the MAP4K4 gene was selected as a candidate gene to evaluate its effect on somatic cell count and milk traits in ChineseHolstein cows. Milk production traits including milk yield, fat percentage, and protein percentage of each cow were collected using 305 d lactation records. Association between MAP4K4 genotype and different traits and Somatic Cell Score (SCS) was performed using General Linear Regression Model of R. Two SNPs at exon 18 (c.2061T > G and c.2196T > C) with genotype TT in both SNPs were found significantly higher for somatic SCS. We found the significant effect of exon 18 (c.2061T > G) on protein percentage, milk yield and SCS. We identified SNPs at different location of MAP4K4 gene of the cattle and several of them were significantly associated with the somatic cell score and other different milk traits. Thus, MAP4K4 gene could be a useful candidate gene for selection of dairy cattle against mastitis and the identified polymorphisms might potentially be strong genetic markers.

  10. Gene-gene interaction and RNA splicing profiles of MAP2K4 gene in rheumatoid arthritis.

    Science.gov (United States)

    Shchetynsky, Klementy; Protsyuk, Darya; Ronninger, Marcus; Diaz-Gallo, Lina-Marcela; Klareskog, Lars; Padyukov, Leonid

    2015-05-01

    We performed gene-gene interaction analysis, with HLA-DRB1 shared epitope (SE) alleles for 195 SNPs within immunologically important MAP2K, MAP3K and MAP4K gene families, in 2010 rheumatoid arthritis (RA) patients and 2280 healthy controls. We found a significant statistical interaction for rs10468473 with SE alleles in autoantibody-positive RA. Individuals heterozygous for rs10468473 demonstrated higher expression of total MAP2K4 mRNA in blood, compared to A-allele homozygous. We discovered a novel, putatively translated, "cassette exon" RNA splice form of MAP2K4, differentially expressed in peripheral blood mononuclear cells from 88 RA cases and controls. Within the group of RA patients, we observed a correlation of MAP2K4 isoform expression with carried SE alleles, autoantibody, and rheumatoid factor profiles. TNF-dependent modulation of isoform expression pattern was detected in the Jurkat cell line. Our data suggest a genetic interaction between MAP2K4 and HLA-DRB1, and the importance of rs10468473 and MAP2K4 splice variants in the development of autoantibody-positive RA.

  11. Transcriptional regulation of the differentiation-linked human K4 promoter is dependent upon esophageal-specific nuclear factors.

    Science.gov (United States)

    Opitz, O G; Jenkins, T D; Rustgi, A K

    1998-09-11

    The stratified squamous epithelium comprises actively proliferating basal cells that undergo a program of differentiation accompanied by morphological, biochemical, and genetic changes. The transcriptional regulatory signals and the genes that orchestrate this switch from proliferation to differentiation can be studied through the keratin gene family. Given the localization of keratin 4 (K4) to the early differentiated suprabasal compartment and having previously demonstrated that targeted disruption of this gene in murine embryonic stem cells results in impairment of the normal differentiation program in esophageal and corneal epithelial cells, we studied the transcriptional regulation of the human K4 promoter. A panel of K4 promoter deletions were found in transient transfection assays to be predominantly active in esophageal and corneal cell lines. A critical cis-regulatory element resides between -163 and -140 bp and contains an inverted CACACCT motif. A site-directed mutated version of this motif within the K4 promoter renders it inactive, whereas the wild-type version is active in a heterologous promoter system. It specifically binds esophageal-specific zinc-dependent transcriptional factors. Our studies demonstrate that regulation of the human K4 promoter is in part mediated through tissue-specific transcriptional factors.

  12. Over-expression of histone H3K4 demethylase gene JMJ15 enhances salt tolerance in Arabidopsis

    Directory of Open Access Journals (Sweden)

    Yuan eShen

    2014-06-01

    Full Text Available Histone H3 lysine 4 trimethylation (H3K4me3 has been shown to be involved in stress-responsive gene expression and gene priming in plants. However, the role of H3K4me3 resetting in the processes is not clear. In this work we studied the expression and function of Arabidopsis H3K4 demethylase gene JMJ15. We show that the expression of JMJ15 was relatively low and was limited to a number of tissues during vegetative growth but was higher in young floral organs. Over-expression of the gene in gain-of-function mutants reduced the plant height with accumulation of lignin in stems, while the loss-of-function mutation did not produce any visible phenotype. The gain-of-function mutants showed enhanced salt tolerance, whereas the loss-of-function mutant was more sensitive to salt compared to the wild type. Transcriptomic analysis revealed that over-expression of JMJ15 down-regulated many genes which are preferentially marked by H3K4me3 and H3K4me2. Many of the down-regulated genes encode transcription regulators involved in stress responses. The data suggest that increased JMJ15 levels may regulate the gene expression program that enhances stress tolerance.

  13. SET DOMAIN GROUP701 encodes a H3K4-methytransferase and regulates multiple key processes of rice plant development.

    Science.gov (United States)

    Liu, Kunpeng; Yu, Yu; Dong, Aiwu; Shen, Wen-Hui

    2017-07-01

    Chromatin-based epigenetic information plays an important role in developmental gene regulation, in response to environment, and in natural variation of gene expression levels. Histone H3 lysine 4 di/trimethylation (H3K4me2/3) is abundant in euchromatin and is generally associated with transcriptional activation. Strikingly, however, enzymes catalyzing H3K4me2/3 remain poorly characterized in crops so far. Here, we investigated the function of the rice SET DOMAIN GROUP 701 (SDG701) gene by molecular and biochemical characterization of the gene product, and by studying effects of its loss or gain of function on plant growth and development. We demonstrated that SDG701 encodes a methytransferase specifically catalyzing H3K4 methylation. Overexpression and knockdown experiments showed that SDG701 is crucial for proper sporophytic plant development as well as for gametophytic transmission that directly impacts rice grain production. In-depth analysis of plant flowering time revealed that SDG701 promotes rice flowering under either long-day or short-day photoperiods. Consistently, the SDG701 protein was found to bind chromatin to promote H3K4me3 and to enhance expression of the rice Hd3a and RFT1 florigens. Collectively, our results establish SDG701 as a major rice H3K4-specific methyltransferase and provide important insights into function of H3K4me3 deposition in transcription activation of florigens in promoting plant flowering. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

  14. K5/K14-positive cells contribute to salivary gland-like breast tumors with myoepithelial differentiation.

    Science.gov (United States)

    Boecker, Werner; Stenman, Goeran; Loening, Thomas; Andersson, Mattias K; Bankfalvi, Agnes; von Holstein, Sarah; Heegaard, Steffen; Lange, Alina; Berg, Tobias; Samoilova, Vera; Tiemann, Katharina; Buchwalow, Igor

    2013-08-01

    Salivary gland-like tumors of the breast show a great variety of architectural patterns and cellular differentiations such as glandular, myoepithelial, squamous, and even mesenchymal phenotypes. However, currently little is known about the evolution and cellular differentiation of these tumors. For that reason, we performed an in situ triple immunofluorescence lineage/differentiation tracing (isTILT) and qRT-PCR study of basal (K5/K14), glandular (K7/K8/18), and epidermal-specific squamous (K10) keratins, p63, and smooth muscle actin (SMA; myoepithelial marker) with the aim to construct and trace different cell lineages and define their cellular hierarchy in tumors with myoepithelial differentiation. isTILT analysis of a series of 28 breast, salivary, and lacrimal gland tumors, including pleomorphic adenomas (n=8), epithelial-myoepithelial tumors (n=9), and adenoid cystic carcinomas (n=11) revealed that all tumor types contained K5/K14-positive progenitor cells in varying frequencies from a few percent up to 15%. These K5/K14-positive tumor cells were found to differentiate to glandular- (K8/18-positive) and myoepithelial-lineage (SMA-positive)-specific cells and were also shown to generate various heterologeous cell differentiations such as squamous and mesenchymal progenies. p63 was co-expressed with K5/K14 in basal-like progenitor cells, myoepithelial, and squamous cells but not in glandular cells. Our results show that the corresponding counterpart tumors of breast and salivary/lacrimal glands have identical cellular compositions. Taken together, our isTILT and RNA-expression data indicate that look-alike tumors of the breast represent a special subgroup of basal-type tumors with benign or usually low malignant potential.

  15. Aberrant intracellular localization of H3k4me3 demonstrates an early epigenetic phenomenon in Alzheimer’s disease

    OpenAIRE

    Mastroeni, Diego; Delvaux, Elaine; Nolz, Jennifer; Tan, Yuyan; Grover, Andrew; Oddo, Salvatore; Coleman, Paul D.

    2015-01-01

    We have previously reported in Alzheimer’s disease (AD) the mislocalization of epigenetic molecules between the cell nucleus and the cytoplasm. We have extended our finding to include the aberrant localization of histone 3 trimethylation on lysine 4 (H3k4me3), an epigenetic mark associated with actively transcribing genes as well as those poised for transcription. These findings raise the question of where the ectopic localization of H3k4me3 fits within the cascade of cell biological events i...

  16. Analisis Cakupan Antenatal Care K4 Program Kesehatan Ibu dan Anak di Wilayah Kerja Dinas Kesehatan Kabupaten Padang Pariaman

    Directory of Open Access Journals (Sweden)

    Elmispendriya Gusna

    2016-01-01

    Full Text Available AbstrakPencapaian target K4 (kunjungan ibu hamil ke tenaga kesehatan yang dilakukan paling sedikit 4 kali selama hamil akan terlaksana jika adanya motivasi bidan di desa ditambah pembinaan dari bidan koordinator yang secara rutin dilakukan dalam bentuk supervisi. Tujuan penelitian ini adalah menganalisis cakupan antenatal care K4 program kesehatan ibu dan anak di wilayah kerja dinas kesehatan kabupaten Padang Pariaman. Penelitian ini menggunakan metode penelitian gabungan antara kuantitatif dan kualitatif. Subjek penelitian untuk penelitian kuantitatif  adalah 49 orang bidan desa, sedangkan informan untuk kualitatif adalah kepala bidang kesehatan keluarga dan Kasie kesehatan ibu dan anak dinas kesehatan kabupaten Padang Pariaman, kepala puskesmas, petugas pemegang program KIA dan bidan pengelola KIA di puskesmas Sungai Limau. Hasil penelitian ini mendapatkan lebih dari separoh (53,1% bidan desa memiliki motivasi rendah, sedangkan lebih dari separoh (67,3% bidan koordinator sudah melakukan supervisi  ke bidan desa dan pada umumnya (91,8% responden memiliki cakupan K4 yang rendah. Tidak terdapat hubungan antara motivasi bidan desa dan supervisi bidan koordinator dengan cakupan antenatal care K4 (p < 0.05. Cakupan antenatal care K4 di Kabupaten Padang Pariaman belum berhasil karena kurangnya peran aktif bidan desa di tengah masyarakat, monitoring dan evaluasi dari dinas kesehatan dan pimpinan puskesmas serta supervisi bidan koordinator belum optimal, serta sumber daya manusia yang belum memaksimalkan perannya dalam melaksanakan tugas ditambah masih kurangnya  kelengkapan sarana dan prasarana. Perlu optimalisasi peran dan fungsi bidan di desa dan optimalisasi kemitraan dengan berbagai pihak dalam komunitas.Kata kunci: cakupan antenatal care K4, motivasi, supervisi AbstractThe achievement of K4 (four time antenatal care target will be success with support from motivation of village midwives and routine supervision of coordinator midwives. The

  17. Draft Genome Sequence of Halomonas elongata Strain K4, an Endophytic Growth-Promoting Bacterium Enhancing Salinity Tolerance In Planta

    KAUST Repository

    Lafi, Feras Fawzi

    2016-11-04

    Halomonas elongata strain K4 is an endophytic bacterial strain that was isolated from roots of Cyperus conglomeratus collected at the Red Sea coast in Thuwal, Saudi Arabia. Here, we present a draft genome sequence of this strain, highlighting a number of pathways involved in plant growth promotion under salt stress.

  18. Draft Genome Sequence of Halomonas elongata Strain K4, an Endophytic Growth-Promoting Bacterium Enhancing Salinity Tolerance In Planta

    Science.gov (United States)

    Lafi, Feras F.; Ramirez-Prado, Juan S.; Alam, Intikhab; Bajic, Vladimir B.

    2016-01-01

    Halomonas elongata strain K4 is an endophytic bacterial strain that was isolated from roots of Cyperus conglomeratus collected at the Red Sea coast in Thuwal, Saudi Arabia. Here, we present a draft genome sequence of this strain, highlighting a number of pathways involved in plant growth promotion under salt stress. PMID:27811099

  19. An efficient solvent-free synthesis of imidazolines and benzimidazoles using K 4[Fe(CN 6] catalysis

    Directory of Open Access Journals (Sweden)

    Kabeer A. Shaikh

    2012-01-01

    Full Text Available Imidazolines and Benzimidazoles have been efficiently synthesized in high yields by treatment of 1,2-diamine with aldehydes using the metal co-ordinate complex K 4[Fe(CN 6] as a catalysis. The method was carried out under solvent free condition via oxidation of carbon-nitrogen bond. The process is green, mild and inexpensive.

  20. The Structure of a High-Affinity Kainate Receptor: GluK4 Ligand-Binding Domain Crystallized with Kainate.

    Science.gov (United States)

    Kristensen, Ole; Kristensen, Lise Baadsgaard; Møllerud, Stine; Frydenvang, Karla; Pickering, Darryl S; Kastrup, Jette Sandholm

    2016-09-01

    Ionotropic glutamate receptors play a key role in fast neurotransmission in the CNS and have been linked to several neurological diseases and disorders. One subfamily is the kainate receptors, which are grouped into low-affinity (GluK1-3) and high-affinity (GluK4-5) receptors based on their affinity for kainate. Although structures of the ligand-binding domain (LBD) of all low-affinity kainate receptors have been reported, no structures of the high-affinity receptor subunits are available. Here, we present the X-ray structure of GluK4-LBD with kainate at 2.05 Å resolution, together with thermofluor and radiolabel binding affinity data. Whereas binding-site residues in GluK4 are most similar to the AMPA receptor subfamily, the domain closure and D1-D2 interlobe contacts induced by kainate are similar to the low-affinity kainate receptor GluK1. These observations provide a likely explanation for the high binding affinity of kainate at GluK4-LBD.

  1. Computational inference of H3K4me3 and H3K27ac domain length

    Directory of Open Access Journals (Sweden)

    Julian Zubek

    2016-03-01

    Full Text Available Background. Recent epigenomic studies have shown that the length of a DNA region covered by an epigenetic mark is not just a byproduct of the assaying technologies and has functional implications for that locus. For example, expanded regions of DNA sequences that are marked by enhancer-specific histone modifications, such as acetylation of histone H3 lysine 27 (H3K27ac domains coincide with cell-specific enhancers, known as super or stretch enhancers. Similarly, promoters of genes critical for cell-specific functions are marked by expanded H3K4me3 domains in the cognate cell type, and these can span DNA regions from 4–5kb up to 40–50kb in length. These expanded H3K4me3 domains are known as buffer domains or super promoters. Methods. To ask what correlates with—and potentially regulates—the length of loci marked with these two important histone marks, H3K4me3 and H3K27ac, we built Random Forest regression models. With these models, we computationally identified genomic and epigenomic patterns that are predictive for the length of these marks in seven ENCODE cell lines. Results. We found that certain epigenetic marks and transcription factors explain the variability of the length of H3K4me3 and H3K27ac marks across different cell types, which implies that the lengths of these two epigenetic marks are tightly regulated in a given cell type. Our source code for the regression models and data can be found at our GitHub page: https://github.com/zubekj/broad_peaks. Discussion. Our Random Forest based regression models enabled us to estimate the individual contribution of different epigenetic marks and protein binding patterns to the length of H3K4me3 and H3K27ac deposition patterns, therefore potentially revealing genomic signatures at cell specific regulatory elements.

  2. Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake.

    Science.gov (United States)

    Pu, L; Zhang, L C; Zhang, J S; Song, X; Wang, L G; Liang, J; Zhang, Y B; Liu, X; Yan, H; Zhang, T; Yue, J W; Li, N; Wu, Q Q; Wang, L X

    2016-08-12

    Mitogen-activated protein kinase kinase kinase 5 (MAP3K5) is essential for apoptosis, proliferation, differentiation, and immune responses, and is a candidate marker for residual feed intake (RFI) in pig. We cloned the full-length cDNA sequence of porcine MAP3K5 by rapid-amplification of cDNA ends. The 5451-bp gene contains a 5'-untranslated region (UTR) (718 bp), a coding region (3738 bp), and a 3'-UTR (995 bp), and encodes a peptide of 1245 amino acids, which shares 97, 99, 97, 93, 91, and 84% sequence identity with cattle, sheep, human, mouse, chicken, and zebrafish MAP3K5, respectively. The deduced MAP3K5 protein sequence contains two conserved domains: a DUF4071 domain and a protein kinase domain. Phylogenetic analysis showed that porcine MAP3K5 forms a separate branch to vicugna and camel MAP3K5. Tissue expression analysis using real-time quantitative polymerase chain reaction (qRT-PCR) revealed that MAP3K5 was expressed in the heart, liver, spleen, lung, kidney, muscle, fat, pancrea, ileum, and stomach tissues. Copy number variation was detected for porcine MAP3K5 and validated by qRT-PCR. Furthermore, a significant increase in average copy number was detected in the low RFI group when compared to the high RFI group in a Duroc pig population. These results provide useful information regarding the influence of MAP3K5 on RFI in pigs.

  3. Histone H3R2 Symmetric Dimethylation and Histone H3K4 Trimethylation Are Tightly Correlated in Eukaryotic Genomes

    OpenAIRE

    Chih-Chi Yuan; Adam G.W. Matthews; Yi Jin; Chang Feng Chen; Brad A. Chapman; Toshiro K. Ohsumi; Karen C. Glass; Tatiana G. Kutateladze; Mark L. Borowsky; Kevin Struhl; Marjorie A. Oettinger

    2012-01-01

    The preferential in vitro interaction of the PHD finger of RAG2, a subunit of the V(D)J recombinase, with histone H3 tails simultaneously trimethylated at lysine 4 and symmetrically dimethylated at arginine 2 (H3R2me2sK4me3) predicted the existence of the previously unknown histone modification H3R2me2s. Here, we report the in vivo identification of H3R2me2s . Consistent with the binding specificity of the RAG2 PHD finger, high levels of H3R2me2sK4me3 are found at antigen receptor gene segmen...

  4. Characterization and analysis of Devonian shales as related to release of gaseous hydrocarbons. Well K-4 Johnson County, Kentucky

    Energy Technology Data Exchange (ETDEWEB)

    Kalyoncu, R.S.; Snyder, M.J.

    1979-08-15

    Various characterization tasks were performed on 54 cored shale samples from Johnson County, Kentucky. Core samples were obtained from depths of 967 to 1510 feet. A total of 126 samples were canned for several DOE contractors, including Battelle Columbus. Free gas analyses indicate the presence of significant quantities of higher chain hydrocarbon gases in the K-4 shales. Hydrocarbon gas release rates and kinetic studies indicate that diffusion coefficients are inversely proportional to the square root of the molecular weight of diffusing species. Although wide scatter is observed in the chemical and physical characterization data, good correlations exist between the hydrocarbon gas contents and various laboratory characterization values (physical and chemical). A number of one-to-one relationships are pointed out and discussed. Lithologically, inorganic portions of K-4 shales are composed predominantly of quartz and illite with small quantities of pyrite and various carbonate minerals.

  5. An expedient Pd/DBU mediated cyanation of aryl/heteroaryl bromides with K4[Fe(CN)6].

    Science.gov (United States)

    Zhang, Dengyou; Sun, Haifeng; Zhang, Lei; Zhou, Yu; Li, Chunpu; Jiang, Hualiang; Chen, Kaixian; Liu, Hong

    2012-03-18

    A practical Pd(PPh(3))(4)/DBU catalytic system for the synthesis of pharmaceutically relevant aminopyridine nitrile intermediates, as well as a variety of other aryl nitriles using non-toxic K(4)[Fe(CN)(6)] has been developed. The key features of our new protocol for cyanation lie in that the reaction can be carried out with readily available Pd(PPh(3))(4) under mild and green conditions, even without the assistance of other ligands.

  6. Aspectos estruturais relacionados ao processo de troca iônica no niobato lamelar K4Nb6O17

    Directory of Open Access Journals (Sweden)

    Marcos A. Bizeto

    2006-12-01

    Full Text Available The niobate with formula K4Nb6O17 has a layered structure formed by stacked negative sheets and exchangeable cations in the interlayer region. In this study we discuss some structural aspects related to the ion exchange in layered hexaniobate based on X-ray diffractometry and vibrational Raman spectroscopy data. Hexaniobate has two distinct interlayer regions and the potassium ions of one interlayer in particular are preferably exchanged by other cations, leading to an interstratified material.

  7. Methylated H3K4, a transcription-associated histone modification, is involved in the DNA damage response pathway.

    Science.gov (United States)

    Faucher, David; Wellinger, Raymund J

    2010-08-26

    Eukaryotic genomes are associated with a number of proteins such as histones that constitute chromatin. Post-translational histone modifications are associated with regulatory aspects executed by chromatin and all transactions on genomic DNA are dependent on them. Thus, it will be relevant to understand how histone modifications affect genome functions. Here we show that the mono ubiquitylation of histone H2B and the tri-methylation of histone H3 on lysine 4 (H3K4me3), both known for their involvement in transcription, are also important for a proper response of budding yeast cells to DNA damaging agents and the passage through S-phase. Cells that cannot methylate H3K4 display a defect in double-strand break (DSB) repair by non-homologous end joining. Furthermore, if such cells incur DNA damage or encounter a stress during replication, they very rapidly lose viability, underscoring the functional importance of the modification. Remarkably, the Set1p methyltransferase as well as the H3K4me3 mark become detectable on a newly created DSB. This recruitment of Set1p to the DSB is dependent on the presence of the RSC complex, arguing for a contribution in the ensuing DNA damage repair process. Taken together, our results demonstrate that Set1p and its substrate H3K4me3, which has been reported to be important for the transcription of active genes, also plays an important role in genome stability of yeast cells. Given the high degree of conservation for the methyltransferase and the histone mark in a broad variety of organisms, these results could have similar implications for genome stability mechanisms in vertebrate and mammalian cells.

  8. [The influence of Acinetobacter calcoaceticus K-4 surface-active substances on the efficiency of microbial destruction of oil pollutants].

    Science.gov (United States)

    Pyroh, T P; Antoniuk, S I; Sorokina, A I

    2009-01-01

    The possibility of the use of Acinetobacter calcoaceticus K-4 surface-active substances (SAS) for water purification from oil was shown. The efficiency of oil degradation (2.6 g/l) in the presence of SAS preparations (5-15 %) in the form of postfermentation of cultural liquid or its supernatant was established to be 81-95 %. Intensification of oil destruction was determined by SAS affecting the activity of oil-oxidizing microbial population.

  9. Synthesis and Structural Determination of Binuclear Nine-Coordinate K4[Y2(Httha)2]·14H2O

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A Y3+ complex with triethylenetetraaminehexaacetic acid was prepared and its composition and structure were determined as K4[Y2(Httha)2]*14H2O by elemental analysis and single-crystal X-ray four circle diffraction analysis. In the binuclear complex, the two YN3O6 parts all form nine-coordinate monocapped square anti prismatic structures. There are a lot of water molecules in the unit cell which form hydrogen bonds.

  10. Methylated H3K4, a transcription-associated histone modification, is involved in the DNA damage response pathway.

    Directory of Open Access Journals (Sweden)

    David Faucher

    2010-08-01

    Full Text Available Eukaryotic genomes are associated with a number of proteins such as histones that constitute chromatin. Post-translational histone modifications are associated with regulatory aspects executed by chromatin and all transactions on genomic DNA are dependent on them. Thus, it will be relevant to understand how histone modifications affect genome functions. Here we show that the mono ubiquitylation of histone H2B and the tri-methylation of histone H3 on lysine 4 (H3K4me3, both known for their involvement in transcription, are also important for a proper response of budding yeast cells to DNA damaging agents and the passage through S-phase. Cells that cannot methylate H3K4 display a defect in double-strand break (DSB repair by non-homologous end joining. Furthermore, if such cells incur DNA damage or encounter a stress during replication, they very rapidly lose viability, underscoring the functional importance of the modification. Remarkably, the Set1p methyltransferase as well as the H3K4me3 mark become detectable on a newly created DSB. This recruitment of Set1p to the DSB is dependent on the presence of the RSC complex, arguing for a contribution in the ensuing DNA damage repair process. Taken together, our results demonstrate that Set1p and its substrate H3K4me3, which has been reported to be important for the transcription of active genes, also plays an important role in genome stability of yeast cells. Given the high degree of conservation for the methyltransferase and the histone mark in a broad variety of organisms, these results could have similar implications for genome stability mechanisms in vertebrate and mammalian cells.

  11. K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidase.

    Science.gov (United States)

    Kobza, Keyna; Camporeale, Gabriela; Rueckert, Brian; Kueh, Alice; Griffin, Jacob B; Sarath, Gautam; Zempleni, Janos

    2005-08-01

    Histones are modified post-translationally, e.g. by methylation of lysine and arginine residues, and by phosphorylation of serine residues. These modifications regulate processes such as gene expression, DNA repair, and mitosis and meiosis. Recently, evidence has been provided that histones are also modified by covalent binding of the vitamin biotin. The aims of this study were to identify biotinylation sites in histone H3, and to investigate the crosstalk among histone biotinylation, methylation and phosphorylation. Synthetic peptides based on the sequence of human histone H3 were used as substrates for enzymatic biotinylation by biotinidase; biotin in peptides was probed using streptavidin peroxidase. These studies provided evidence that K4, K9 and K18 in histone H3 are good targets for biotinylation; K14 and K23 are relatively poor targets. Antibodies were generated to histone H3, biotinylated either at K4, K9 or K18. These antibodies localized to nuclei in human placental cells in immunocytochemistry and immunoblotting experiments, suggesting that lysines in histone H3 are biotinylated in vivo. Dimethylation of R2, R8 and R17 increased biotinylation of K4, K9 and K18, respectively, by biotinidase; phosphorylation of S10 abolished biotinylation of K9. These observations are consistent with crosstalk between biotinylation of histones and other known modifications of histones. We speculate that this crosstalk provides a link to known roles for biotin in gene expression and cell proliferation.

  12. PHF20 Readers Link Methylation of Histone H3K4 and p53 with H4K16 Acetylation

    Directory of Open Access Journals (Sweden)

    Brianna J. Klein

    2016-10-01

    Full Text Available PHF20 is a core component of the lysine acetyltransferase complex MOF (male absent on the first-NSL (non-specific lethal that generates the major epigenetic mark H4K16ac and is necessary for transcriptional regulation and DNA repair. The role of PHF20 in the complex remains elusive. Here, we report on functional coupling between methylation readers in PHF20. We show that the plant homeodomain (PHD finger of PHF20 recognizes dimethylated lysine 4 of histone H3 (H3K4me2 and represents an example of a native reader that selects for this modification. Biochemical and structural analyses help to explain this selectivity and the preference of Tudor2, another reader in PHF20, for dimethylated p53. Binding of the PHD finger to H3K4me2 is required for histone acetylation, accumulation of PHF20 at target genes, and transcriptional activation. Together, our findings establish a unique PHF20-mediated link between MOF histone acetyltransferase (HAT, p53, and H3K4me2, and suggest a model for rapid spreading of H4K16ac-enriched open chromatin.

  13. PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3.

    Science.gov (United States)

    Chung, Ho-Ryun; Xu, Chao; Fuchs, Alisa; Mund, Andreas; Lange, Martin; Staege, Hannah; Schubert, Tobias; Bian, Chuanbing; Dunkel, Ilona; Eberharter, Anton; Regnard, Catherine; Klinker, Henrike; Meierhofer, David; Cozzuto, Luca; Winterpacht, Andreas; Di Croce, Luciano; Min, Jinrong; Will, Hans; Kinkley, Sarah

    2016-05-25

    PHF13 is a chromatin affiliated protein with a functional role in differentiation, cell division, DNA damage response and higher chromatin order. To gain insight into PHF13's ability to modulate these processes, we elucidate the mechanisms targeting PHF13 to chromatin, its genome wide localization and its molecular chromatin context. Size exclusion chromatography, mass spectrometry, X-ray crystallography and ChIP sequencing demonstrate that PHF13 binds chromatin in a multivalent fashion via direct interactions with H3K4me2/3 and DNA, and indirectly via interactions with PRC2 and RNA PolII. Furthermore, PHF13 depletion disrupted the interactions between PRC2, RNA PolII S5P, H3K4me3 and H3K27me3 and resulted in the up and down regulation of genes functionally enriched in transcriptional regulation, DNA binding, cell cycle, differentiation and chromatin organization. Together our findings argue that PHF13 is an H3K4me2/3 molecular reader and transcriptional co-regulator, affording it the ability to impact different chromatin processes.

  14. Preparation and antioxidant activities of a sulfated derivative of exopolysaccharide from Escherichia coil K5%Escherichia coli K5胞外多糖硫酸酯衍生物的制备及其体外抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    王华; 徐静静; 曹凤; 陈敬华

    2012-01-01

    对Escherichia coliK5菌株进行发酵培养,发酵液上清经超滤、醇沉、Sevage法脱蛋白、透析冻干,再经DEAE琼脂糖柱、G75葡聚糖柱分离纯化后得到K5多糖(K5)。以三氧化硫吡啶为硫酸酯化剂,对K5多糖N位进行了硫酸酯化,制备了K5多糖的硫酸酯衍生物(NS-K5),二糖分析结果显示,N位硫酸酯化率达到57%。并对硫酸酯化前后的K5多糖的抗氧化性能进行了研究,结果显示,一定浓度范围内,NS-K5多糖还原力较K5高,当K5和NS-K5浓度达到1mg/mL时,对羟基自由基的清除率分别达到17.7%、25.6%,对DPPH自由基的清除率分别达到26.1%、45%。实验结果表明,NS-K5的抗氧化活性要高于K5。%Escherichia coil K5 strain was cultivated by fermentation,the supernatant was treated by ultrafiltration, exopolysaccharide(EPS) was extracted by ethanol precipitation,and then purified by Sevage method,dialysis, freeze-dried, DEAE-Sepharose and Sephadex G-75 column,finally high-purity K5 polysaccharide(K5) was obtained. N site of the K5 polysaccharide was chemically sulfated by Sulfur trioxide pyridine complex,NS-K5 polysaccharide(NS-K5) was prepared,and disaccharide analysis revealed that N-sulfation rate was up to 57%. The antioxidant capacity of K5 polysaccharideand N-sulfated K5 polysaccharide was investigated,the results showed that within a certain concentration range,the reducing activity of NS-K5 was powerful over K5, when the concentration of K5 and NS-K5 was up to lmg/mL,the hydroxyl radical scavenging rates were 17.7% ,25.6% ,and DPPH radical scavenging rates were 26.1% ,45% ,respectively. The result indicated that NS-K5 showed stronger antioxidant activity than K5.

  15. Enhancer-associated H3K4 monomethylation by trithorax-related, the drosophila homolog of mammalian MLL3/MLL4

    NARCIS (Netherlands)

    H.-M. Herz (Hans-Martin); M. Mohan (Man); A.S. Garruss (Alexander); K. Liang (Kaiwei); Y.-H. Takahashi (Yoh-hei); K. Mickey (Kristen); O. Voets (Olaf); C.P. Verrijzer (Peter); A. Shilatifard (Ali)

    2012-01-01

    textabstractMonomethylation of histone H3 on Lys 4 (H3K4me1) and acetylation of histone H3 on Lys 27 (H3K27ac) are histone modifications that are highly enriched over the body of actively transcribed genes and on enhancers. Although in yeast all H3K4 methylation patterns, including H3K4me1, are impl

  16. K5/K14-positive cells contribute to salivary gland-like breast tumors with myoepithelial differentiation

    DEFF Research Database (Denmark)

    Boecker, Werner; Stenman, Goeran; Loening, Thomas

    2013-01-01

    Salivary gland-like tumors of the breast show a great variety of architectural patterns and cellular differentiations such as glandular, myoepithelial, squamous, and even mesenchymal phenotypes. However, currently little is known about the evolution and cellular differentiation of these tumors....... For that reason, we performed an in situ triple immunofluorescence lineage/differentiation tracing (isTILT) and qRT-PCR study of basal (K5/K14), glandular (K7/K8/18), and epidermal-specific squamous (K10) keratins, p63, and smooth muscle actin (SMA; myoepithelial marker) with the aim to construct and trace...... different cell lineages and define their cellular hierarchy in tumors with myoepithelial differentiation. isTILT analysis of a series of 28 breast, salivary, and lacrimal gland tumors, including pleomorphic adenomas (n=8), epithelial-myoepithelial tumors (n=9), and adenoid cystic carcinomas (n=11) revealed...

  17. 图K(5m,5)的自同态的自同态谱%On the Andomorphism Spectrum of K(5m,5)

    Institute of Scientific and Technical Information of China (English)

    余鋆

    2012-01-01

    In this paper, we obtain that Aut(K(5m, 5)) ≈D5m,sEnd(k(5m, 5))=Aut(k(5m, 5) and End(K(5m,5)) =qEnd(K(5m, 5)), where Ds. is the dihedral group of order 5m. Furthermore, we solve some enumerative problems of End(K(5m, 5)), and give the endomorphism spectrum of k(5m, 5).%从循环完全图K(5m,5)的结构出发,得到Aut(K(5m,5))≈D5m,其中D5m是5m阶的二面体群,sEnd(k(5m,5))=Aut(k(5m,5),End(K(5m,5))=qEnd(K(5m,5))。同时也解决了End(K(5m,5))的一些记数问题,给出了此类图的自同态谱。

  18. Morphed and moving: TNFα-driven motility promotes cell dissemination through MAP4K4-induced cytoskeleton remodeling

    Directory of Open Access Journals (Sweden)

    Min Ma

    2014-04-01

    Full Text Available Cell dissemination from an initial site of growth is a highly coordinated and controlled process that depends on cell motility. The mechanistic principles that orchestrate cell motility, namely cell shape control, traction and force generation, are highly conserved between cells of different origins. Correspondingly, the molecular mechanisms that regulate these critical aspects of migrating cells are likely functionally conserved too. Thus, cell motility deregulation of unrelated pathogenesis could be caused and maintained by similar mechanistic principles. One such motility deregulation disorder is the leukoproliferative cattle disease Tropical Theileriosis, which is caused by the intracellular, protozoan parasite Theileria annulata. T. annulata transforms its host cell and promotes the dissemination of parasite-infected cells throughout the body of the host. An analogous condition with a fundamentally different pathogenesis is metastatic cancer, where oncogenically transformed cells disseminate from the primary tumor to form distant metastases. Common to both diseases is the dissemination of motile cells from the original site. However, unlike metastatic cancer, host cell transformation by Theileria parasites can be reverted by drug treatment and cell signaling be analyzed under transformed and non-transformed conditions. We have used this reversible transformation model and investigated parasite control of host cell motile properties in the context of inflammatory signaling in Ma M. et al. [PLoS Pathog (2014 10: e1004003]. We found that parasite infection promotes the production of the inflammatory cytokine TNFα in the host macrophage. We demonstrated that increased TNFα triggers motile and invasive properties by enhancing actin cytoskeleton remodeling and cell motility through the ser/thr kinase MAP4K4. We concluded that inflammatory conditions resulting in increased TNFα could facilitate cell dissemination by activating the actin

  19. Association of common genetic variants in the MAP4K4 locus with prediabetic traits in humans.

    Directory of Open Access Journals (Sweden)

    Tina Sartorius

    Full Text Available Mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4 is expressed in all diabetes-relevant tissues and mediates cytokine-induced insulin resistance. We investigated whether common single nucleotide polymorphisms (SNPs in the MAP4K4 locus associate with glucose intolerance, insulin resistance, impaired insulin release, or elevated plasma cytokines. The best hit was tested for association with type 2 diabetes. Subjects (N = 1,769 were recruited from the Tübingen Family (TÜF study for type 2 diabetes and genotyped for tagging SNPs. In a subgroup, cytokines were measured. Association with type 2 diabetes was tested in a prospective case-cohort study (N = 2,971 derived from the EPIC-Potsdam study. Three SNPs (rs6543087, rs17801985, rs1003376 revealed nominal and two SNPs (rs11674694, rs11678405 significant associations with 2-hour glucose levels. SNPs rs6543087 and rs11674694 were also nominally associated with decreased insulin sensitivity. Another two SNPs (rs2236936, rs2236935 showed associations with reduced insulin release, driven by effects in lean subjects only. Three SNPs (rs11674694, rs13003883, rs2236936 revealed nominal associations with IL-6 levels. SNP rs11674694 was significantly associated with type 2 diabetes. In conclusion, common variation in MAP4K4 is associated with insulin resistance and β-cell dysfunction, possibly via this gene's role in inflammatory signalling. This variation's impact on insulin sensitivity may be more important since its effect on insulin release vanishes with increasing BMI.

  20. The Oryza sativa Regulator HDR1 Associates with the Kinase OsK4 to Control Photoperiodic Flowering.

    Directory of Open Access Journals (Sweden)

    Xuehui Sun

    2016-03-01

    Full Text Available Rice is a facultative short-day plant (SDP, and the regulatory pathways for flowering time are conserved, but functionally modified, in Arabidopsis and rice. Heading date 1 (Hd1, an ortholog of Arabidopsis CONSTANS (CO, is a key regulator that suppresses flowering under long-day conditions (LDs, but promotes flowering under short-day conditions (SDs by influencing the expression of the florigen gene Heading date 3a (Hd3a. Another key regulator, Early heading date 1 (Ehd1, is an evolutionarily unique gene with no orthologs in Arabidopsis, which acts as a flowering activator under both SD and LD by promoting the rice florigen genes Hd3a and RICE FLOWERING LOCUST 1 (RFT1. Here, we report the isolation and characterization of the flowering regulator Heading Date Repressor1 (HDR1 in rice. The hdr1 mutant exhibits an early flowering phenotype under natural LD in a paddy field in Beijing, China (39°54'N, 116°23'E, as well as under LD but not SD in a growth chamber, indicating that HDR1 may functionally regulate flowering time via the photoperiod-dependent pathway. HDR1 encodes a nuclear protein that is most active in leaves and floral organs and exhibits a typical diurnal expression pattern. We determined that HDR1 is a novel suppressor of flowering that upregulates Hd1 and downregulates Ehd1, leading to the downregulation of Hd3a and RFT1 under LDs. We have further identified an HDR1-interacting kinase, OsK4, another suppressor of rice flowering under LDs. OsK4 acts similarly to HDR1, suppressing flowering by upregulating Hd1 and downregulating Ehd1 under LDs, and OsK4 can phosphorylate HD1 with HDR1 presents. These results collectively reveal the transcriptional regulators of Hd1 for the day-length-dependent control of flowering time in rice.

  1. The Oryza sativa Regulator HDR1 Associates with the Kinase OsK4 to Control Photoperiodic Flowering.

    Science.gov (United States)

    Sun, Xuehui; Zhang, Zhiguo; Wu, Jinxia; Cui, Xuean; Feng, Dan; Wang, Kai; Xu, Ming; Zhou, Li; Han, Xiao; Gu, Xiaofeng; Lu, Tiegang

    2016-03-01

    Rice is a facultative short-day plant (SDP), and the regulatory pathways for flowering time are conserved, but functionally modified, in Arabidopsis and rice. Heading date 1 (Hd1), an ortholog of Arabidopsis CONSTANS (CO), is a key regulator that suppresses flowering under long-day conditions (LDs), but promotes flowering under short-day conditions (SDs) by influencing the expression of the florigen gene Heading date 3a (Hd3a). Another key regulator, Early heading date 1 (Ehd1), is an evolutionarily unique gene with no orthologs in Arabidopsis, which acts as a flowering activator under both SD and LD by promoting the rice florigen genes Hd3a and RICE FLOWERING LOCUST 1 (RFT1). Here, we report the isolation and characterization of the flowering regulator Heading Date Repressor1 (HDR1) in rice. The hdr1 mutant exhibits an early flowering phenotype under natural LD in a paddy field in Beijing, China (39°54'N, 116°23'E), as well as under LD but not SD in a growth chamber, indicating that HDR1 may functionally regulate flowering time via the photoperiod-dependent pathway. HDR1 encodes a nuclear protein that is most active in leaves and floral organs and exhibits a typical diurnal expression pattern. We determined that HDR1 is a novel suppressor of flowering that upregulates Hd1 and downregulates Ehd1, leading to the downregulation of Hd3a and RFT1 under LDs. We have further identified an HDR1-interacting kinase, OsK4, another suppressor of rice flowering under LDs. OsK4 acts similarly to HDR1, suppressing flowering by upregulating Hd1 and downregulating Ehd1 under LDs, and OsK4 can phosphorylate HD1 with HDR1 presents. These results collectively reveal the transcriptional regulators of Hd1 for the day-length-dependent control of flowering time in rice.

  2. Activity measurements of the radionuclides 18F and 99mTc for the NMISA, South Africa in the ongoing comparisons BIPM.RI(II)-K4.F-18 and BIPM.RI(II)-K4.Tc-99m

    Science.gov (United States)

    Michotte, C.; Nonis, M.; Van Rooy, M. W.; Van Staden, M. J.; Lubbe, J.

    2017-01-01

    In 2015, comparisons of activity measurements of 18F and 99mTc using the Transfer Instrument of the International Reference System (SIRTI) took place at the National Metrology Institute of South Africa (NMISA, South Africa). Ampoules containing about 25 kBq of 18F and 99mTc solutions were measured in the SIRTI for more than two half-lives. The NMISA standardized the activity in the ampoules by ionization chamber measurements traceable to 4π(LS)β-γ coincidence measurements. The comparisons, identifiers BIPM.RI(II)-K4.F-18 and BIPM.RI(II)-K4.Tc-99m, are linked to the corresponding BIPM.RI(II)-K1.F-18 and BIPM.RI(II)-K1.Tc-99m comparisons and degrees of equivalence with the respective key comparison reference values have been evaluated. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCRI, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  3. The H3K4me3/2 histone demethylase RBR-2 controls axon guidance by repressing the actin-remodeling gene wsp-1

    DEFF Research Database (Denmark)

    Mariani, Luca; Lussi, Yvonne C.; Vandamme, Julien;

    2016-01-01

    . Here, we show that RBR-2, the sole homolog of the KDM5 family of H3K4me3/2 demethylases in Caenorhabditis elegans, ensures correct axon guidance by controlling the expression of the actin regulator wsp-1. Loss of rbr-2 results in increased levels of H3K4me3 at the transcriptional start site of wsp-1...

  4. Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae)

    Science.gov (United States)

    Serrano, Érica Alves; Araya-Jaime, Cristian; Suárez-Villota, Elkin Y.; Oliveira, Claudio; Foresti, Fausto

    2016-01-01

    Abstract Characidium gomesi Travasso, 1956 specimens from the Pardo River have up to four heterochromatic supernumerary chromosomes, derived from the sex chromosomes. To access the meiotic behavior and distribution of an active chromatin marker, males and females of Characidium gomesi with two or three B chromosomes were analyzed. Mitotic chromosomes were characterized using C-banding and FISH with B chromosome probes. Meiocytes were subjected to immunofluorescence-FISH assay using anti-SYCP3, anti-H3K4m, and B chromosomes probes. Molecular homology of supernumeraries was confirmed by FISH and by its bivalent conformation in individuals with two of these chromosomes. In individuals with three Bs, these elements formed a bivalent and a univalent. Supernumerary and sex chromosomes exhibited H3K4m signals during pachytene contrasting with their heterochromatic and asynaptic nature, which suggest a more structural role than functional of this histone modification. The implications of this result are discussed in light of the homology, meiotic nuclear organization, and meiotic silencing of unsynapsed chomatin. PMID:27551347

  5. Trithorax monomethylates histone H3K4 and interacts directly with CBP to promote H3K27 acetylation and antagonize Polycomb silencing.

    Science.gov (United States)

    Tie, Feng; Banerjee, Rakhee; Saiakhova, Alina R; Howard, Benny; Monteith, Kelsey E; Scacheri, Peter C; Cosgrove, Michael S; Harte, Peter J

    2014-03-01

    Trithorax (TRX) antagonizes epigenetic silencing by Polycomb group (PcG) proteins, stimulates enhancer-dependent transcription, and establishes a 'cellular memory' of active transcription of PcG-regulated genes. The mechanisms underlying these TRX functions remain largely unknown, but are presumed to involve its histone H3K4 methyltransferase activity. We report that the SET domains of TRX and TRX-related (TRR) have robust histone H3K4 monomethyltransferase activity in vitro and that Tyr3701 of TRX and Tyr2404 of TRR prevent them from being trimethyltransferases. The trx(Z11) missense mutation (G3601S), which abolishes H3K4 methyltransferase activity in vitro, reduces the H3K4me1 but not the H3K4me3 level in vivo. trx(Z11) also suppresses the impaired silencing phenotypes of the Pc(3) mutant, suggesting that H3K4me1 is involved in antagonizing Polycomb silencing. Polycomb silencing is also antagonized by TRX-dependent H3K27 acetylation by CREB-binding protein (CBP). We show that perturbation of Polycomb silencing by TRX overexpression requires CBP. We also show that TRX and TRR are each physically associated with CBP in vivo, that TRX binds directly to the CBP KIX domain, and that the chromatin binding patterns of TRX and TRR are highly correlated with CBP and H3K4me1 genome-wide. In vitro acetylation of H3K27 by CBP is enhanced on K4me1-containing H3 substrates, and independently altering the H3K4me1 level in vivo, via the H3K4 demethylase LSD1, produces concordant changes in H3K27ac. These data indicate that the catalytic activities of TRX and CBP are physically coupled and suggest that both activities play roles in antagonizing Polycomb silencing, stimulating enhancer activity and cellular memory.

  6. Characterization of a cold-active lipase from Psychrobacter cryohalolentis K5(T) and its deletion mutants.

    Science.gov (United States)

    Novototskaya-Vlasova, K A; Petrovskaya, L E; Rivkina, E M; Dolgikh, D A; Kirpichnikov, M P

    2013-04-01

    A gene coding for cold-active lipase from the psychrotrophic Gram-negative bacterium Psychrobacter cryohalolentis K5(T) isolated from a Siberian cryopeg has been cloned and expressed in Escherichia coli. The recombinant protein Lip1Pc with a 6× histidine tag at its C-terminus was purified by nickel affinity chromatography. With p-nitrophenyl dodecanoate (C12) as a substrate, the purified recombinant protein displayed maximum lipolytic activity at 25°C and pH 8.0. Increasing the temperature above 40°C and addition of various metal ions and organic solvents inhibited the enzymatic activity of Lip1Pc. Most nonionic detergents, such as Triton X-100 and Tween 20, slightly increased the lipase activity, while SDS completely inhibited it. To investigate the functional significance of the Lip1Pc N-terminal domain, we constructed five deletion mutants of this protein. The ND1 and ND2 mutants displayed specific activity reduced by 30-35%, while other truncated proteins were completely inactive. Both mutants demonstrated increased activity towards p-nitrophenyl decanoate (C10) and impaired utilization of C16 substrate. Although optimum reaction temperature of ND2 lowered to 20°C, it displayed enhanced stability by 44% after incubation at 40°C. The results prove that the N-terminal domain of Lip1Pc has a fundamental impact on the activity and stability of the protein.

  7. Expression and chaperone-assisted refolding of a new cold-active lipase from Psychrobacter cryohalolentis K5(T).

    Science.gov (United States)

    Novototskaya-Vlasova, Ksenia; Petrovskaya, Lada; Kryukova, Elena; Rivkina, Elizaveta; Dolgikh, Dmitry; Kirpichnikov, Mikhail

    2013-09-01

    We describe cloning and expression of genes coding for lipase Lip2Pc and lipase-specific foldase LifPc from a psychrotrophic microorganism Psychrobacter cryohalolentis K5(T) isolated from a Siberian cryopeg (the lense of overcooled brine within permafrost). Upon expression in Escherichiacoli Lip2Pc accumulated in inclusion bodies while chaperone was synthesized in a soluble form. An efficient protocol for solubilization and subsequent refolding of the recombinant lipase in the presence of the truncated chaperone was developed. Using this procedure Lip2Pc with specific activity of 6900U/mg was obtained. Contrary to published data on other lipase-chaperone complexes, refolded Lip2Pc was mostly recovered from the complex with chaperone by metal-affinity chromatography. Recombinant Lip2Pc displayed maximum lipolytic activity at 25°C and pH 8.0 with p-nitrophenyl palmitate (C16) as a substrate. Activity assays conducted at different temperatures revealed that the recombinant Lip2Pc is a cold-adapted lipase with ability to utilize substrates with long (C10-C16) hydrocarbon chains in the temperature range from +5 to +65°C. It demonstrated relatively high stability at temperatures above 60°C and in the presence of various metal ions or organic solvents (ethanol, methanol, etc.). Non-ionic detergents, such as Triton X-100 and Tween 20 decreased Lip2Pc activity and SDS completely inhibited it.

  8. Probing M subdwarf metallicity with an esdK5+esdM5.5 binary

    CERN Document Server

    Pavlenko, Ya V; Gálvez-Ortiz, M C; Kushniruk, I O; Jones, H R A

    2015-01-01

    We present a spectral analysis of the binary G 224-58 AB that consists of the coolest M extreme subdwarf (esdM5.5) and a brighter primary (esdK5). This binary may serve as a benchmark for metallicity measurement calibrations and as a test-bed for atmospheric and evolutionary models for esdM objects. We determine abundances primarily using high resolution optical spectra of the primary. Other parameters were determined from the fits of synthetic spectra computed with these abundances to the observed spectra from 0.4 to 2.5 microns for both components. We determine \\Tef =4625 $\\pm$ 100 K, \\logg = 4.5 $\\pm$ 0.5 for the A component and \\Tef = 3200 $\\pm$ 100 K, \\logg = 5.0 $\\pm$ 0.5, for the B component. We obtained abundances of [Mg/H]=$-$1.51$\\pm$0.08, [Ca/H]=$-$1.39$\\pm$0.03, [Ti/H]=$-$1.37$\\pm$0.03 for alpha group elements and [CrH]=$-$1.88$\\pm$0.07, [Mn/H]=$-$1.96$\\pm$0.06, [Fe/H]=$-$1.92$\\pm$0.02, [Ni/H]=$-$1.81$\\pm$0.05 and [Ba/H]W=$-$1.87$\\pm$0.11 for iron group elements from fits to the spectral lines obs...

  9. K4MY METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  10. K4BM METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  11. K4BL METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  12. Discussion for the Innovation of Automatic Welding Practice Education Based on K5 Demonstrate Education Method%基于K5示教法的自动化焊接实践教学改革初探

    Institute of Scientific and Technical Information of China (English)

    曲芳; 沈斌; 岳旭东; 刘存宪; 海波; 高路

    2016-01-01

    在自动化焊接的学生实践教学环节中,学校需投入大量的人力、物力和财力,以保障实践教学工作的有序进行。然而,部分高校由于受到各方面条件的限制,不得不减少或取消实践教学环节,这对于提高学生的实践动手能力造成了很大的阻碍。拟利用黑龙江科技大学实训中心现有设备:奥地利IGM公司的RTI330型自动化焊接机器人,针对该校材料学院焊接专业在校学生,采用K5示教器模拟实践操作环节,通过人机交互界面以示教的方法让学生更多地去感受实际焊接过程,使更多学生得到实践锻炼的机会,为自动化焊接实践环节节省了材料和设备。在整个教学环节中,既发挥了教师的主导作用,又体现了学生的认知主体作用,真正实现了产学一体化,学做合一,为用人单位培养出适应行业发展的应用型焊接人才。%In the segment of the students' automatic welding practice education, the universities need to investment in generous manpower, material and wealth in order to insure the practice education work to carry out smoothly. However, part of the universities are enslaved to the conditions in all direction, they have to reduce or cancel the segment of the practice education, this action creates impediment of enhancing the students’practice manipulative ability. We plan to use the existing automatic welding robot equipment of the center of training and practice in our university, which is from IGM company of Austria with the version of RTI330, focuses on the students whose major is welding of Material Science Institute, use K5 demonstrate education implement to simulation the segment of the practice manipulation, by means of human-computer interaction interface and the demonstrate education method to let the students to feel the reality welding process still more, get more chances of practice and save more materials and equipments for the

  13. Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation

    Energy Technology Data Exchange (ETDEWEB)

    Bian, Chuanbing; Xu, Chao; Ruan, Jianbin; Lee, Kenneth K.; Burke, Tara L.; Tempel, Wolfram; Barsyte, Dalia; Li, Jing; Wu, Minhao; Zhou, Bo O.; Fleharty, Brian E.; Paulson, Ariel; Allali-Hassani, Abdellah; Zhou, Jin-Qiu; Mer, Georges; Grant, Patrick A.; Workman, Jerry L.; Zang, Jianye; Min, Jinrong (Toronto); (Stowers); (UST - China); (UV); (Chinese Aca. Sci.); (MCCM)

    2011-09-28

    The SAGA (Spt-Ada-Gcn5 acetyltransferase) complex is an important chromatin modifying complex that can both acetylate and deubiquitinate histones. Sgf29 is a novel component of the SAGA complex. Here, we report the crystal structures of the tandem Tudor domains of Saccharomyces cerevisiae and human Sgf29 and their complexes with H3K4me2 and H3K4me3 peptides, respectively, and show that Sgf29 selectively binds H3K4me2/3 marks. Our crystal structures reveal that Sgf29 harbours unique tandem Tudor domains in its C-terminus. The tandem Tudor domains in Sgf29 tightly pack against each other face-to-face with each Tudor domain harbouring a negatively charged pocket accommodating the first residue alanine and methylated K4 residue of histone H3, respectively. The H3A1 and K4me3 binding pockets and the limited binding cleft length between these two binding pockets are the structural determinants in conferring the ability of Sgf29 to selectively recognize H3K4me2/3. Our in vitro and in vivo functional assays show that Sgf29 recognizes methylated H3K4 to recruit the SAGA complex to its targets sites and mediates histone H3 acetylation, underscoring the importance of Sgf29 in gene regulation.

  14. The RING-CH ligase K5 antagonizes restriction of KSHV and HIV-1 particle release by mediating ubiquitin-dependent endosomal degradation of tetherin.

    Directory of Open Access Journals (Sweden)

    Claire Pardieu

    2010-04-01

    Full Text Available Tetherin (CD317/BST2 is an interferon-induced membrane protein that inhibits the release of diverse enveloped viral particles. Several mammalian viruses have evolved countermeasures that inactivate tetherin, with the prototype being the HIV-1 Vpu protein. Here we show that the human herpesvirus Kaposi's sarcoma-associated herpesvirus (KSHV is sensitive to tetherin restriction and its activity is counteracted by the KSHV encoded RING-CH E3 ubiquitin ligase K5. Tetherin expression in KSHV-infected cells inhibits viral particle release, as does depletion of K5 protein using RNA interference. K5 induces a species-specific downregulation of human tetherin from the cell surface followed by its endosomal degradation. We show that K5 targets a single lysine (K18 in the cytoplasmic tail of tetherin for ubiquitination, leading to relocalization of tetherin to CD63-positive endosomal compartments. Tetherin degradation is dependent on ESCRT-mediated endosomal sorting, but does not require a tyrosine-based sorting signal in the tetherin cytoplasmic tail. Importantly, we also show that the ability of K5 to substitute for Vpu in HIV-1 release is entirely dependent on K18 and the RING-CH domain of K5. By contrast, while Vpu induces ubiquitination of tetherin cytoplasmic tail lysine residues, mutation of these positions has no effect on its antagonism of tetherin function, and residual tetherin is associated with the trans-Golgi network (TGN in Vpu-expressing cells. Taken together our results demonstrate that K5 is a mechanistically distinct viral countermeasure to tetherin-mediated restriction, and that herpesvirus particle release is sensitive to this mode of antiviral inhibition.

  15. Cytoskeletal studies on Lowicryl K4M embedded and Affi-Gel 731 attached rat peritoneal mast cells.

    Science.gov (United States)

    Nielsen, E H; Jahn, H

    1984-01-01

    The subplasmalemmal network in mast cells consists of irregularly arranged 6-7 nm filaments (actin) connected by thinner filaments. In places oblique filaments with crossbridges or short, perpendicular filaments (11-12 nm) connect cell and granule membrane. Filaments attaching subplasmalemmal network to cell membrane divide like a Y and attach cell membrane end-on with a conical, hook-like bending. Each granule is surrounded by a regular network of filaments.

  16. Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2

    DEFF Research Database (Denmark)

    Pasini, Diego; Hansen, Klaus H; Christensen, Jesper;

    2008-01-01

    Polycomb group (PcG) proteins regulate important cellular processes such as embryogenesis, cell proliferation, and stem cell self-renewal through the transcriptional repression of genes determining cell fate decisions. The Polycomb-Repressive Complex 2 (PRC2) is highly conserved during evolution......, and its intrinsic histone H3 Lys 27 (K27) trimethylation (me3) activity is essential for PcG-mediated transcriptional repression. Here, we show a functional interplay between the PRC2 complex and the H3K4me3 demethylase Rbp2 (Jarid1a) in mouse embryonic stem (ES) cells. By genome-wide location analysis we...... found that Rbp2 is associated with a large number of PcG target genes in mouse ES cells. We show that the PRC2 complex recruits Rbp2 to its target genes, and that this interaction is required for PRC2-mediated repressive activity during ES cell differentiation. Taken together, these results demonstrate...

  17. KEY COMPARISON: Final report on key comparison APMP.QM-K4.1: Ethanol in nitrogen

    Science.gov (United States)

    Kato, Kenji; Maruyama, Masaaki; Seog Kim, Jin; Hyub, Oh Sang; Woo, Jin-Chun; Kim, Yongdoo; Bae, Hyunkil; Han, Qiao; Zhou, Zeyi

    2008-01-01

    The aim of this comparison was to compare capabilities for the preparation and value assignment of gas mixture standards for ethanol in nitrogen, maintained at national metrology institutes (NMIs). The measurements in this key comparison took place in 2006. There were three participants and one coordinating laboratory. The key comparison reference value (KCRV) was based on the gravimetric preparation for all components. The range of the nominal amount-of-substance fractions of the comparison standard is 100 µmol/mol to 130 µmol/mol. The Chemical Evaluation Research Institute (CERI, Japan) prepared gravimetric mixture samples of ethanol in nitrogen in 10 L aluminium cylinders and the homogeneity and long-term stability of these samples were evaluated at CERI before and after the shipment of the cylinders to the participants. Each participating laboratory was shipped one cylinder to be analyzed and then returned to the CERI. Each sample was reanalyzed to verify whether the contents were stable throughout the comparison. These measurements showed no significant change in the amount fraction within the estimated uncertainty of the measurements. Two of the three participants submitted results that were within 0.1% of the relevant reference value. In these cases, the estimated uncertainty was larger than the deviation from the reference value. CERI was the link laboratory between CCQM-K4 and APMP.QM-K4. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (MRA).

  18. Evaluation of a multiplex PCR for detection of serotypes K1, K2 and K5 in Klebsiella sp. and comparison of isolates within these serotypes.

    Science.gov (United States)

    Turton, Jane F; Baklan, Hatice; Siu, L K; Kaufmann, Mary E; Pitt, Tyrone L

    2008-07-01

    A multiplex PCR using targets within the serotype-specific region of the capsular polysaccharide synthesis gene cluster of serotypes K1, K2 and K5 was evaluated using the 77 reference serotype strains of Klebsiella, and a panel of clinical isolates subjected previously to conventional serotyping. The PCR was highly specific for these serotypes, which are those most associated with virulence in humans and horses. PCR confirmed that isolates of the K5 serotype had cross-reacted with antiserum for other serotypes, particularly for K7. K5 isolates received by our laboratory were almost exclusively from thoroughbred horses, and were submitted for screening prior to breeding programmes. Most, including a reference strain isolated in 1955, belonged to a cluster of genetically similar isolates of sequence type (ST) 60. K1 isolates, all from humans, belonged to a previously identified cluster of ST 23.

  19. Recombinant Escherichia coli K5 strain with the deletion of waaR gene decreases the molecular weight of the heparosan capsular polysaccharide.

    Science.gov (United States)

    Huang, Haichan; Liu, Xiaobo; Lv, Shencong; Zhong, Weihong; Zhang, Fuming; Linhardt, Robert J

    2016-09-01

    Heparosan, the capsular polysaccharide of Escherichia coli K5 having a carbohydrate backbone similar to that of heparin, has become a potential precursor for bioengineering heparin. In the heparosan biosynthesis pathway, the gene waaR encoding α-1-, 2- glycosyltransferase catalyze s the third glucosyl residues linking to the oligosaccharide chain. In the present study, a waaR deletion mutant of E. coli K5 was constructed. The mutant showed improvement of capsule polysaccharide yield. It is interesting that the heparosan molecular weight of the mutant is reduced and may become more suitable as a precursor for the production of low molecular weight heparin derived from the wild-type K5 capsular polysaccharide.

  20. High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production

    OpenAIRE

    Catapano Angela; De Rosa Mario; Cimini Donatella; Restaino Odile; Schiraldi Chiara

    2011-01-01

    Abstract Background The bacteria Escherichia coli K4 produces a capsular polysaccharide (K4 CPS) whose backbone is similar to the non sulphated chondroitin chain. The chondroitin sulphate is one of the major components of the extra-cellular matrix of the vertebrate connective tissues and a high value molecule, widely employed as active principle in the treatment of osteoarthritis. It is usually obtained by extraction from animal tissues, but the risk of virus contaminations, as well as the sc...

  1. Weissella paramesenteroides WpK4 reduces gene expression of intestinal cytokines, and hepatic and splenic injuries in a murine model of typhoid fever.

    Science.gov (United States)

    Alvim, L B; Sandes, S H C; Silva, B C; Steinberg, R S; Campos, M H A; Acurcio, L B; Arantes, R M E; Nicoli, J R; Neumann, E; Nunes, Á C

    2016-02-01

    Diarrhoea in piglets by Salmonella and other pathogens can be a serious health problem. Non-drug treatments such as probiotic microorganisms have various effects on the gastrointestinal microbiota dysbiosis and host immune system modulation. The aim of this study was to demonstrate the suitable use of Weissella paramesenteroides WpK4 strain isolated from healthy piglets as an alternative prophylactic or therapeutic treatment against Salmonella Typhimurium. Out of 37 lactic acid bacteria isolates, 24 strains belonging to the Weissella and Lactobacillus genera were analysed in vitro for desirable probiotic characteristics. The W. paramesenteroides WpK4 strain fulfilled all in vitro tests: resistance to acidic pH and bile salts, hydrophobic cell surface, antagonism against bacterial pathogens, H2O2 production and exopolysaccharide secretion, and non-transferable resistance to antibiotics. Mice fed with WpK4 showed no signs of bacterial translocation to the liver or spleen and decreased Salmonella translocation to these organs. Significantly, WpK4 intake attenuated the weight loss, fostered the preservation of intestinal architecture and integrity, and promoted survival in mice following infection with Salmonella Typhimurium. In addition, WpK4 modulated immune cellular response by inhibiting the production of pro-inflammatory cytokines and inducing anti-inflammatory mediators. These findings validate the probiotic properties of W. paramesenteroides WpK4 strain, and its eventual use in piglets.

  2. 65-nm Cyclone Ⅲ FPGA

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Altera公司低功耗、低成本Cyclone Ⅲ系列65nm FPGA所有8个型号的产品级芯片实现量产,Cyclone Ⅲ系列产品已迅速应用于无线、军事、显示、汽车和工业市场的大量客户系统中。

  3. Macrohistone Variants Preserve Cell Identity by Preventing the Gain of H3K4me2 during Reprogramming to Pluripotency

    Directory of Open Access Journals (Sweden)

    María J. Barrero

    2013-04-01

    Full Text Available Transcription-factor-induced reprogramming of somatic cells to pluripotency is a very inefficient process, probably due to the existence of important epigenetic barriers that are imposed during differentiation and that contribute to preserving cell identity. In an effort to decipher the molecular nature of these barriers, we followed a genome-wide approach, in which we identified macrohistone variants (macroH2A as highly expressed in human somatic cells but downregulated after reprogramming to pluripotency, as well as strongly induced during differentiation. Knockdown of macrohistone variants in human keratinocytes increased the efficiency of reprogramming to pluripotency, whereas overexpression had opposite effects. Genome-wide occupancy profiles show that in human keratinocytes, macroH2A.1 preferentially occupies genes that are expressed at low levels and are marked with H3K27me3, including pluripotency-related genes and bivalent developmental regulators. The presence of macroH2A.1 at these genes prevents the regain of H3K4me2 during reprogramming, imposing an additional layer of repression that preserves cell identity.

  4. Remarks on Vertex-Distinguishing IE-Total Coloring of Complete Bipartite Graphs K4,n and Kn,n

    Institute of Scientific and Technical Information of China (English)

    Xiang'en CHEN; Xiaoqing XIN; Wenyu HE

    2012-01-01

    Let G be a simple graph.An IE-total coloring f of G refers to a coloring of the vertices and edges of G so that no two adjacent vertices receive the same color.Let C(u) be the set of colors of vertex u and edges incident to u under f.For an IE-total coloring f of G using k colors,if C(u) ≠ C(v) for any two different vertices u and v of V(G),then f is called a k-vertex-distinguishing IE-total-coloring of G,or a k-VDIET coloring of G for short.The minimum number of colors required for a VDIET coloring of G is denoted by xievt(G),and it is called the VDIET chromatic number of G.We will give VDIET chromatic numbers for complete bipartite graph K4,n (n ≥ 4),Kn,n (5 ≤ n ≤ 21) in this article.

  5. Decreased H3K27 and H3K4 trimethylation on mortal chromosomes in distributed stem cells.

    Science.gov (United States)

    Huh, Y H; Sherley, J L

    2014-12-04

    The role of immortal DNA strands that co-segregate during mitosis of asymmetrically self-renewing distributed stem cells (DSCs) is unknown. Previously, investigation of immortal DNA strand function and molecular mechanisms responsible for their nonrandom co-segregation was precluded by difficulty in identifying DSCs and immortal DNA strands. Here, we report the use of two technological innovations, selective DSC expansion and establishment of H2A.Z chromosomal asymmetry as a specific marker of 'immortal chromosomes,' to investigate molecular properties of immortal chromosomes and opposing 'mortal chromosomes' in cultured mouse hair follicle DSCs. Although detection of the respective suppressive and activating H3K27me3 and H3K4me3 epigenetic marks on immortal chromosomes was similar to randomly segregated chromosomes, detection of both was lower on mortal chromosomes destined for lineage-committed sister cells. This global epigenomic feature of nonrandom co-segregation may reveal a mechanism that maintains an epigenome-wide 'poised' transcription state, which preserves DSC identity, while simultaneously activating sister chromosomes for differentiation.

  6. Modeling Math Growth Trajectory--An Application of Conventional Growth Curve Model and Growth Mixture Model to ECLS K-5 Data

    Science.gov (United States)

    Lu, Yi

    2016-01-01

    To model students' math growth trajectory, three conventional growth curve models and three growth mixture models are applied to the Early Childhood Longitudinal Study Kindergarten-Fifth grade (ECLS K-5) dataset in this study. The results of conventional growth curve model show gender differences on math IRT scores. When holding socio-economic…

  7. Preparation, characterization and photocatalytic studies of Cu2+, Sn2+ and N3− substituted K5Sb5P2O20

    Indian Academy of Sciences (India)

    Ch Sudhakar Reddy; Sreenu K; J R Reddy; A Hari Padmasri; Ravi Gundeboina; M Vithal

    2016-04-01

    Potassium antimony phosphates (K-Sb-P-O) exhibit different structural networks and therefore they were studied as photocatalysts in the present investigation. K5Sb5P2O20 was prepared by solid state method. Metal ions (Cu2+ and Sn2+), and non-metal anion, N3−, were substituted into the K5Sb5P2O20 for possible enhancement of photocatalytic activity. The precursor and substituted compounds were characterized by powder X-ray diffraction, FT-IR, SEM-EDS and UV-Vis diffuse reflectance spectra. Nitrogen substitution into K5Sb5P2O20 lattice was studied by O-N-H and XPS measurements. The photocatalytic activity of all the compounds was studied by degradation of methylene blue and methyl violet. The ion-substituted K5Sb5P2O20 have shown higher photocatalytic activity against both the dyes. The role of reactive intermediate species produced in the photocatalytic reaction was studied using their appropriate scavengers.

  8. Expanding the Role of K-5 Science Instruction in Educational Reform: Implications of an Interdisciplinary Model for Integrating Science and Reading

    Science.gov (United States)

    Romance, Nancy R.; Vitale, Michael R.

    2012-01-01

    Addressed is the current practice in educational reform of reducing time for science instruction in favor of traditional reading/language arts instruction. In contrast, presented is an evidence-based rationale for increasing instructional time for K-5 science instruction as an educational reform initiative. Overviewed are consensus…

  9. High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production

    Directory of Open Access Journals (Sweden)

    De Rosa Mario

    2011-02-01

    Full Text Available Abstract Background The bacteria Escherichia coli K4 produces a capsular polysaccharide (K4 CPS whose backbone is similar to the non sulphated chondroitin chain. The chondroitin sulphate is one of the major components of the extra-cellular matrix of the vertebrate connective tissues and a high value molecule, widely employed as active principle in the treatment of osteoarthritis. It is usually obtained by extraction from animal tissues, but the risk of virus contaminations, as well as the scarceness of raw material, makes this productive process unsafe and unable to satisfy the growing market demand. In previous studies a new biotechnological process to produce chondroitin from Escherichia coli K4 capsular polysaccharide was investigated and a 1.4 g·L-1 K4 CPS concentration was reached using fed-batch fermentation techniques. In this work, on the trail of these results, we exploited new fermentation strategies to further improve the capsular polysaccharide production. Results The inhibitory effect of acetate on the bacterial cells growth and K4 CPS production was studied in shake flask conditions, while a new approach, that combined the optimization of the feeding profiles, the improvement of aeration conditions and the use of a microfiltration bioreactor, was investigated in three different types of fermentation processes. High polysaccharide concentrations (4.73 ± 0.2 g·L-1, with corresponding average yields (0.13 ± 0.006 gK4 CPS·gcdw-1, were obtained; the increase of K4 CPS titre, compared to batch and fed-batch results, was of 16-fold and 3.3-fold respectively, while average yield was almost 3.5 and 1.4 fold higher. Conclusion The increase of capsular polysaccharide titre confirmed the validity of the proposed fermentation strategy and opened the way to the use of the microfiltration bioreactor for the biotechnological production of chondroitin.

  10. Exploring PHD fingers and H3K4me0 interactions with molecular dynamics simulations and binding free energy calculations: AIRE-PHD1, a comparative study.

    Directory of Open Access Journals (Sweden)

    Dimitrios Spiliotopoulos

    Full Text Available PHD fingers represent one of the largest families of epigenetic readers capable of decoding post-translationally modified or unmodified histone H3 tails. Because of their direct involvement in human pathologies they are increasingly considered as a potential therapeutic target. Several PHD/histone-peptide structures have been determined, however relatively little information is available on their dynamics. Studies aiming to characterize the dynamic and energetic determinants driving histone peptide recognition by epigenetic readers would strongly benefit from computational studies. Herein we focus on the dynamic and energetic characterization of the PHD finger subclass specialized in the recognition of histone H3 peptides unmodified in position K4 (H3K4me0. As a case study we focused on the first PHD finger of autoimmune regulator protein (AIRE-PHD1 in complex with H3K4me0. PCA analysis of the covariance matrix of free AIRE-PHD1 highlights the presence of a "flapping" movement, which is blocked in an open conformation upon binding to H3K4me0. Moreover, binding free energy calculations obtained through Molecular Mechanics/Poisson-Boltzmann Surface Area (MM/PBSA methodology are in good qualitative agreement with experiments and allow dissection of the energetic terms associated with native and alanine mutants of AIRE-PHD1/H3K4me0 complexes. MM/PBSA calculations have also been applied to the energetic analysis of other PHD fingers recognizing H3K4me0. In this case we observe excellent correlation between computed and experimental binding free energies. Overall calculations show that H3K4me0 recognition by PHD fingers relies on compensation of the electrostatic and polar solvation energy terms and is stabilized by non-polar interactions.

  11. Infection Exposure Promotes ETV6-RUNX1 Precursor B-cell Leukemia via Impaired H3K4 Demethylases.

    Science.gov (United States)

    Rodríguez-Hernández, Guillermo; Hauer, Julia; Martín-Lorenzo, Alberto; Schäfer, Daniel; Bartenhagen, Christoph; García-Ramírez, Idoia; Auer, Franziska; González-Herrero, Inés; Ruiz-Roca, Lucia; Gombert, Michael; Okpanyi, Vera; Fischer, Ute; Chen, Cai; Dugas, Martin; Bhatia, Sanil; Linka, René Martin; Garcia-Suquia, Marta; Rascón-Trincado, María Victoria; Garcia-Sanchez, Angel; Blanco, Oscar; García-Cenador, Maria Begoña; García-Criado, Francisco Javier; Cobaleda, César; Alonso-López, Diego; De Las Rivas, Javier; Müschen, Markus; Vicente-Dueñas, Carolina; Sánchez-García, Isidro; Borkhardt, Arndt

    2017-08-15

    ETV6-RUNX1 is associated with the most common subtype of childhood leukemia. As few ETV6-RUNX1 carriers develop precursor B-cell acute lymphocytic leukemia (pB-ALL), the underlying genetic basis for development of full-blown leukemia remains to be identified, but the appearance of leukemia cases in time-space clusters keeps infection as a potential causal factor. Here, we present in vivo genetic evidence mechanistically connecting preleukemic ETV6-RUNX1 expression in hematopoetic stem cells/precursor cells (HSC/PC) and postnatal infections for human-like pB-ALL. In our model, ETV6-RUNX1 conferred a low risk of developing pB-ALL after exposure to common pathogens, corroborating the low incidence observed in humans. Murine preleukemic ETV6-RUNX1 pro/preB cells showed high Rag1/2 expression, known for human ETV6-RUNX1 pB-ALL. Murine and human ETV6-RUNX1 pB-ALL revealed recurrent genomic alterations, with a relevant proportion affecting genes of the lysine demethylase (KDM) family. KDM5C loss of function resulted in increased levels of H3K4me3, which coprecipitated with RAG2 in a human cell line model, laying the molecular basis for recombination activity. We conclude that alterations of KDM family members represent a disease-driving mechanism and an explanation for RAG off-target cleavage observed in humans. Our results explain the genetic basis for clonal evolution of an ETV6-RUNX1 preleukemic clone to pB-ALL after infection exposure and offer the possibility of novel therapeutic approaches. Cancer Res; 77(16); 4365-77. ©2017 AACR. ©2017 American Association for Cancer Research.

  12. Sal k 4, a new allergen of Salsola kali, is profilin: a predictive value of conserved conformational regions in cross-reactivity with other plant-derived profilins.

    Science.gov (United States)

    Assarehzadegan, Mohammad Ali; Amini, Akram; Sankian, Mojtaba; Tehrani, Mohsen; Jabbari, Farahzad; Varasteh, Abdolreza

    2010-01-01

    The aim of this study was to investigate a new allergen of Salsola kali, Sal k 4, and to investigate the predictive value of the conserved conformational regions in cross-reactivity with other plant-derived profilins. The Sal k 4-coding sequence was cloned, expressed, and purified by one-step Ni2+ affinity chromatography to recover high-purity target protein. We assessed cross-reactivity and predicted conserved conformational regions among rSal k 4 and other plant-derived profilins. Immunodetection and inhibition assays using 30 individual sera from S. kali allergic patients indicated that purified rSal k 4 might be the same as that in the crude extract. The results of inhibition assays among rSal k 4 and other plant-derived profilins were in accordance with the homology of the predicted conserved conformational regions. Amino acid sequence homology analysis showed that a high degree of IgE cross-reactivity among plant-derived profilins might depend on the predicted conserved conformational regions.

  13. The Histone Demethylase Jarid1b Ensures Faithful Mouse Development by Protecting Developmental Genes from Aberrant H3K4me3

    DEFF Research Database (Denmark)

    Albert, Mareike; Schmitz, Sandra U; Kooistra, Susanne M

    2013-01-01

    of the H3K4me2/3 histone demethylase Jarid1b (Kdm5b/Plu1) results in major neonatal lethality due to respiratory failure. Jarid1b knockout embryos have several neural defects including disorganized cranial nerves, defects in eye development, and increased incidences of exencephaly. Moreover, in line...... with an overlap of Jarid1b and Polycomb target genes, Jarid1b knockout embryos display homeotic skeletal transformations typical for Polycomb mutants, supporting a functional interplay between Polycomb proteins and Jarid1b. To understand how Jarid1b regulates mouse development, we performed a genome-wide analysis...... of histone modifications, which demonstrated that normally inactive genes encoding developmental regulators acquire aberrant H3K4me3 during early embryogenesis in Jarid1b knockout embryos. H3K4me3 accumulates as embryonic development proceeds, leading to increased expression of neural master regulators like...

  14. Altered expression of BRG1 and histone demethylases, and aberrant H3K4 methylation in less developmentally competent embryos at the time of embryonic genome activation.

    Science.gov (United States)

    Glanzner, Werner G; Wachter, Audrey; Coutinho, Ana Rita S; Albornoz, Marcelo S; Duggavathi, Raj; GonÇAlves, Paulo B D; Bordignon, Vilceu

    2017-01-01

    Epigenetics is a fundamental regulator underlying many biological functions, such as development and cell differentiation. Epigenetic modifications affect key chromatin regulation, including transcription and DNA repair, which are critical for normal embryo development. In this study, we profiled the expression of epigenetic modifiers and patterns of epigenetic changes in porcine embryos around the period of embryonic genome activation (EGA). We observed that Brahma-related gene 1 (BRG1) and Lysine demethylase 1A (KDM1A), which can alter the methylation status of lysine 4 in histone 3 (H3K4), localize to the nucleus at Day 3-4 of development. We then compared the abundance of epigenetic modifiers between early- and late-cleaving embryos, which were classified based on the time to the first cell cleavage, to investigate if their nuclear localization contributes to developmental competence. The mRNA abundance of BRG1, KDM1A, as well as other lysine demethylases (KDM1B, KDM5A, KDM5B, and KDM5C), were significantly higher in late- compared to early-cleaving embryos near the EGA period, although these difference disappeared at the blastocyst stage. The abundance of H3K4 mono- (H3K4me) and di-methylation (H3K4me2) during the EGA period was reduced in late-cleaving and less developmentally competent embryos. By contrast, BRG1, KDM1A, and H3K4me2 abundance was greater in embryos with more than eight cells at Day 3-4 of development compared to those with fewer than four cells. These findings suggest that altered epigenetic modifications of H3K4 around the EGA period may affect the developmental capacity of porcine embryos to reach the blastocyst stage. Mol. Reprod. Dev. 84: 19-29, 2017. © 2016 Wiley Periodicals, Inc.

  15. Epigenetic Profiling of H3K4Me3 Reveals Herbal Medicine Jinfukang-Induced Epigenetic Alteration Is Involved in Anti-Lung Cancer Activity.

    Science.gov (United States)

    Lu, Jun; Zhang, Xiaoli; Shen, Tingting; Ma, Chao; Wu, Jun; Kong, Hualei; Tian, Jing; Shao, Zhifeng; Zhao, Xiaodong; Xu, Ling

    2016-01-01

    Traditional Chinese medicine Jinfukang (JFK) has been clinically used for treating lung cancer. To examine whether epigenetic modifications are involved in its anticancer activity, we performed a global profiling analysis of H3K4Me3, an epigenomic marker associated with active gene expression, in JFK-treated lung cancer cells. We identified 11,670 genes with significantly altered status of H3K4Me3 modification following JFK treatment (P JFK. Collectively, these findings provide the first evidence that the anticancer activity of JFK involves modulation of histone modification at many cancer-related gene loci.

  16. Condensation patterns of prophase/prometaphase chromosome are correlated with H4K5 histone acetylation and genomic DNA contents in plants.

    Science.gov (United States)

    Feitoza, Lidiane; Costa, Lucas; Guerra, Marcelo

    2017-01-01

    Mitotic prophase chromosome condensation plays an essential role in nuclear division being therefore regulated by highly conserved mechanisms. However, degrees of chromatin condensation in prophase-prometaphase cells may vary along the chromosomes resulting in specific condensation patterns. We examined different condensation patterns (CPs) of prophase and prometaphase chromosomes and investigated their relationship with genome size and distribution of histone H4 acetylated at lysine 5 (H4K5ac) in 17 plant species. Our results showed that most species with small genomes (2C < 5 pg) (Arachis pusilla, Bixa orellana, Costus spiralis, Eleutherine bulbosa, Indigofera campestris, Phaseolus lunatus, P. vulgaris, Poncirus trifoliata, and Solanum lycopersicum) displayed prophase chromosomes with late condensing terminal regions that were highly enriched in H4K5ac, and early condensing regions with apparently non-acetylated proximal chromatin. The species with large genomes (Allium cepa, Callisia repens, Araucaria angustifolia and Nothoscordum pulchellum) displayed uniformly condensed and acetylated prophase/prometaphase chromosomes. Three species with small genomes (Eleocharis geniculata, Rhynchospora pubera, and R. tenuis) displayed CP and H4K5ac labeling patterns similar to species with large genomes, whereas a forth species (Emilia sonchifolia) exhibited a gradual chromosome labeling, being more acetylated in the terminal regions and less acetylated in the proximal ones. The nucleolus organizer chromatin was the only chromosomal region that in prometaphase or metaphase could be hyperacetylated, hypoacetylated or non-acetylated, depending on the species. Our data indicate that the CP of a plant chromosome complement is influenced but not exclusively determined by nuclear and chromosomal DNA contents, whereas the CP of individual chromosomes is clearly correlated with H4K5ac distribution.

  17. 调控因子SlyA和RfaH在E.coli K4合成果糖软骨素中的生理作用%Effects of overexpression of transcriptional regulators SlyA and RfaH on fructosylated chondroitin production in E coli K4

    Institute of Scientific and Technical Information of China (English)

    杨爱华; 刘佳; 张震宇; 刘立明

    2015-01-01

    To enhance K4CPS production of E. coli K4,the transcriptional factor SlyA and RfaH were over expressed in E. coli K4 to create E. coli pTH⁃slyA,E. coli pTH⁃rfaH and co⁃expression strain E. coli pTH⁃slyA⁃rfaH. The over⁃expression of SlyA and RfaH significantly enhanced K4CPS production. The K4CPS concentration for E. coli pTH⁃slyA,E. coli pTH⁃rfaH and E. coli pTH⁃slyA⁃rfaH was increased by 81�8%、46�5% and 153�8%, compared with that of E. coli K4, respectively. The glycerol consumption rate of recombinant strain decreased by 12�5%、18�8% and 31�3% than that of E. coli K4. Moreover, the concentration of acetic acid decreased by 98�6%、39�7%and 91�6%compared with that of E. coli K4� The overexpression of slyA and rfaH could significantly enhanced K4CPS production.%利用基因工程手段过量表达E. coli K4荚膜多糖( K4CPS)合成途径中相关代谢调控因子SlyA和RfaH,以构建高效合成K4CPS的菌株。结果发现:slyA和rfaH基因的单独过量表达和共表达重组菌的K4CPS的产量较对照菌分别提高了81�8%、46�5%和153�8%;甘油比消耗速率较对照菌降低了12�5%、18�8%和31�3%,而乙酸含量则较对照菌降低了98�6%、39�7%和91�6%。因此,调控蛋白SlyA和RfaH的过量表达能够大幅度提高E�coli K4K4CPS合成,并增强菌体对甘油的利用率,而乙酸合成明显降低。而与单独表达策略相比,共表达策略能够更加有效地增强菌体合成K4CPS的能力。

  18. An HST COS 'SNAPSHOT' spectrum of the K supergiant λ Vel (K4Ib-II)

    Energy Technology Data Exchange (ETDEWEB)

    Carpenter, Kenneth G. [NASA/GSFC Code 667, Greenbelt, MD 20771 (United States); Ayres, Thomas R. [University of Colorado, CASA, 389-UCB, Boulder, CO 80309 (United States); Harper, Graham M. [School of Physics, Trinity College, Dublin 2 (Ireland); Kober, Gladys; Nielsen, Krister E.; Wahlgren, Glenn M., E-mail: Kenneth.G.Carpenter@nasa.gov [Deptartment of Physics, Catholic University of America, Washington, DC 20064 (United States)

    2014-10-10

    We present a far-ultraviolet spectrum of the K4 Ib-II supergiant λ Vel obtained with the Hubble Space Telescope's Cosmic Origins Spectrograph (COS) as a part of the SNAPshot program 'SNAPing coronal iron' (GO 11687). The observation covers a wavelength region (1326-1467 Å) not previously recorded for λ Vel at a spectral resolving power of R ∼ 20,000 and displays strong emission and absorption features, superposed on a bright chromospheric continuum. Fluorescent excitation is responsible for much of the observed emission, mainly powered by strong H I Lyα and the O I (UV 2) triplet emission near λ1304. The molecular CO and H{sub 2} fluorescences are weaker than in the early-K giant α Boo while the Fe II and Cr II lines, also pumped by H I Lyα, are stronger in λ Vel. This pattern of relative line strengths between the two stars is explained by the lower iron-group element abundance in α Boo, which weakens that star's Fe II and Cr II emission without reducing the molecular fluorescences. The λ Vel spectrum shows fluorescent Fe II, Cr II, and H{sub 2} emission similar to that observed in the M supergiant α Ori, but more numerous well-defined narrow emissions from CO. The additional CO emissions are visible in the spectrum of λ Vel since that star does not have the cool, opaque circumstellar shells that surround α Ori and produce broad circumstellar CO (A-X) band absorptions that hide those emissions in the cooler star. The presence of Si IV emission in λ Vel indicates a ∼8 × 10{sup 4} K plasma that is mixed into the cooler chromosphere. Evidence of the stellar wind is seen in the C II λλ1334,1335 lines and in the blueshifted Fe II and Ni II wind absorption lines. Line modeling using Sobolev with Exact Integration for the C II lines indicates a larger terminal velocity (∼45 versus ∼30 km s{sup –1}) and turbulence (∼27 versus <21 km s{sup –1}) with a more quickly accelerating wind (β = 0.35 versus 0.7) at the time of

  19. The ATRX-ADD domain binds to H3 tail peptides and reads the combined methylation state of K4 and K9

    Science.gov (United States)

    Dhayalan, Arunkumar; Tamas, Raluca; Bock, Ina; Tattermusch, Anna; Dimitrova, Emilia; Kudithipudi, Srikanth; Ragozin, Sergey; Jeltsch, Albert

    2011-01-01

    Mutations in the ATRX protein are associated with the alpha-thalassemia and mental retardation X-linked syndrome (ATR-X). Almost half of the disease-causing mutations occur in its ATRX-Dnmt3-Dnmt3L (ADD) domain. By employing peptide arrays, chromatin pull-down and peptide binding assays, we show specific binding of the ADD domain to H3 histone tail peptides containing H3K9me3. Peptide binding was disrupted by the presence of the H3K4me3 and H3K4me2 modification marks indicating that the ATRX-ADD domain has a combined readout of these two important marks (absence of H3K4me2 and H3K4me3 and presence of H3K9me3). Disease-causing mutations reduced ATRX-ADD binding to H3 tail peptides. ATRX variants, which fail in the H3K9me3 interaction, show a loss of heterochromatic localization in cells, which indicates the chromatin targeting function of the ADD domain of ATRX. Disruption of H3K9me3 binding may be a general pathogenicity pathway of ATRX mutations in the ADD domain which may explain the clustering of disease mutations in this part of the ATRX protein. PMID:21421568

  20. The ATRX-ADD domain binds to H3 tail peptides and reads the combined methylation state of K4 and K9.

    Science.gov (United States)

    Dhayalan, Arunkumar; Tamas, Raluca; Bock, Ina; Tattermusch, Anna; Dimitrova, Emilia; Kudithipudi, Srikanth; Ragozin, Sergey; Jeltsch, Albert

    2011-06-01

    Mutations in the ATRX protein are associated with the alpha-thalassemia and mental retardation X-linked syndrome (ATR-X). Almost half of the disease-causing mutations occur in its ATRX-Dnmt3-Dnmt3L (ADD) domain. By employing peptide arrays, chromatin pull-down and peptide binding assays, we show specific binding of the ADD domain to H3 histone tail peptides containing H3K9me3. Peptide binding was disrupted by the presence of the H3K4me3 and H3K4me2 modification marks indicating that the ATRX-ADD domain has a combined readout of these two important marks (absence of H3K4me2 and H3K4me3 and presence of H3K9me3). Disease-causing mutations reduced ATRX-ADD binding to H3 tail peptides. ATRX variants, which fail in the H3K9me3 interaction, show a loss of heterochromatic localization in cells, which indicates the chromatin targeting function of the ADD domain of ATRX. Disruption of H3K9me3 binding may be a general pathogenicity pathway of ATRX mutations in the ADD domain which may explain the clustering of disease mutations in this part of the ATRX protein.

  1. The Meiotic Recombination Activator PRDM9 Trimethylates Both H3K36 and H3K4 at Recombination Hotspots In Vivo.

    Science.gov (United States)

    Powers, Natalie R; Parvanov, Emil D; Baker, Christopher L; Walker, Michael; Petkov, Petko M; Paigen, Kenneth

    2016-06-01

    In many mammals, including humans and mice, the zinc finger histone methyltransferase PRDM9 performs the first step in meiotic recombination by specifying the locations of hotspots, the sites of genetic recombination. PRDM9 binds to DNA at hotspots through its zinc finger domain and activates recombination by trimethylating histone H3K4 on adjacent nucleosomes through its PR/SET domain. Recently, the isolated PR/SET domain of PRDM9 was shown capable of also trimethylating H3K36 in vitro, raising the question of whether this reaction occurs in vivo during meiosis, and if so, what its function might be. Here, we show that full-length PRDM9 does trimethylate H3K36 in vivo in mouse spermatocytes. Levels of H3K4me3 and H3K36me3 are highly correlated at hotspots, but mutually exclusive elsewhere. In vitro, we find that although PRDM9 trimethylates H3K36 much more slowly than it does H3K4, PRDM9 is capable of placing both marks on the same histone molecules. In accord with these results, we also show that PRDM9 can trimethylate both K4 and K36 on the same nucleosomes in vivo, but the ratio of K4me3/K36me3 is much higher for the pair of nucleosomes adjacent to the PRDM9 binding site compared to the next pair further away. Importantly, H3K4me3/H3K36me3-double-positive nucleosomes occur only in regions of recombination: hotspots and the pseudoautosomal (PAR) region of the sex chromosomes. These double-positive nucleosomes are dramatically reduced when PRDM9 is absent, showing that this signature is PRDM9-dependent at hotspots; the residual double-positive nucleosomes most likely come from the PRDM9-independent PAR. These results, together with the fact that PRDM9 is the only known mammalian histone methyltransferase with both H3K4 and H3K36 trimethylation activity, suggest that trimethylation of H3K36 plays an important role in the recombination process. Given the known requirement of H3K36me3 for double strand break repair by homologous recombination in somatic cells, we

  2. MoSET1 (Histone H3K4 Methyltransferase in Magnaporthe oryzae Regulates Global Gene Expression during Infection-Related Morphogenesis.

    Directory of Open Access Journals (Sweden)

    Kieu Thi Minh Pham

    2015-07-01

    Full Text Available Here we report the genetic analyses of histone lysine methyltransferase (KMT genes in the phytopathogenic fungus Magnaporthe oryzae. Eight putative M. oryzae KMT genes were targeted for gene disruption by homologous recombination. Phenotypic assays revealed that the eight KMTs were involved in various infection processes at varying degrees. Moset1 disruptants (Δmoset1 impaired in histone H3 lysine 4 methylation (H3K4me showed the most severe defects in infection-related morphogenesis, including conidiation and appressorium formation. Consequently, Δmoset1 lost pathogenicity on wheat host plants, thus indicating that H3K4me is an important epigenetic mark for infection-related gene expression in M. oryzae. Interestingly, appressorium formation was greatly restored in the Δmoset1 mutants by exogenous addition of cAMP or of the cutin monomer, 16-hydroxypalmitic acid. The Δmoset1 mutants were still infectious on the super-susceptible barley cultivar Nigrate. These results suggested that MoSET1 plays roles in various aspects of infection, including signal perception and overcoming host-specific resistance. However, since Δmoset1 was also impaired in vegetative growth, the impact of MoSET1 on gene regulation was not infection specific. ChIP-seq analysis of H3K4 di- and tri-methylation (H3K4me2/me3 and MoSET1 protein during infection-related morphogenesis, together with RNA-seq analysis of the Δmoset1 mutant, led to the following conclusions: 1 Approximately 5% of M. oryzae genes showed significant changes in H3K4-me2 or -me3 abundance during infection-related morphogenesis. 2 In general, H3K4-me2 and -me3 abundance was positively associated with active transcription. 3 Lack of MoSET1 methyltransferase, however, resulted in up-regulation of a significant portion of the M. oryzae genes in the vegetative mycelia (1,491 genes, and during infection-related morphogenesis (1,385 genes, indicating that MoSET1 has a role in gene repression either

  3. Final report on COOMET.M.M-K5: Key comparison in the field of multiples and submultiples of the kilogram (COOMET 258/RU/02)

    Science.gov (United States)

    Snegov, V. S.

    2014-01-01

    This report summarizes the results of a comparison of mass standards conducted between four participating members of the Euro-Asian Cooperation of National metrological Institutes (COOMET). The COOMET key comparisons are usually carried out on those schemes that were proposed and implemented by the Consultative Committee for Mass and Related Quantities of the CIPM. Measurement comparisons bring to the laboratories confidence in the measurement standards and lead to their international acceptance. This comparison arose from discussions at the 9th meeting of TC 1.6 'Mass and Related Quantities' on 21-23 April 2004, SMU, Slovakia. The comparison is internally identified as COOMET 258/RU/02 and corresponds to the KCDB identifier COOMET.M.M-K5, as attributed by the BIPM. The purpose of the key comparison COOMET.M.M-K5 'Comparison in the field of multiples and submultiples of the kilogram' is to compare the results of measurements obtained by the participants. Two weights sets with five nominal mass values 2 kg, 200 g, 50 g, 1 g and 200 mg (OIML Class E1) were used as transfer standards. These nominal values were chosen to follow the second phase of the CCM key comparison CCM.M-K5. The All-Russian D I Mendeleyev Research Institute for Metrology, VNIIM, was the pilot laboratory as well as the linking laboratory to CCM.M-K5. The comparison was carried out from May 2015 to July 2008. In 2007, measurements were carried out by Kazakhstan Institute of Metrology (RSE 'KazInMetr') on their own initiative. The computation of the degrees of equivalence shows a generally good agreement between participants. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  4. Synthesis of PbS Intercalated K4Nb6O17 Composite and Its Photocatalytic Activity for Hydrogen Production%PbS插层K4Nb6O17复合物的制备及其光催化制氢活性

    Institute of Scientific and Technical Information of China (English)

    崔文权; 刘艳飞; 胡金山; 刘利; 梁英华

    2012-01-01

    采用固相法合成层状半导体K4Nb6O17,通过层间离子交换反应、胺插入反应以及硫化反应制备了纳米PbS插层的K4Nb6O17复合光催化剂(记作K4Nb6O17/PbS).利用X射线衍射(XRD),场发射扫描电镜(SEM),X射线荧光光谱仪(XRF),紫外-可见漫反射(UV-Vis)和分子荧光光谱(PL)等技术对其进行表征.考察了催化剂在Na2SO3和Na2S为牺牲剂的光催化制氢活性.结果表明,PbS的插层拓展了K4Nb6O17对可见光的响应,催化制氢活性也有明显提高.在紫外光和可见光下3h产氢量分别达到123.94和0.66 mmol/(g cat).最后讨论了插层复合催化剂光生电荷转移的机理.%The layered compound K4Nb6O17 was prepared via high temperature solid reaction, and PbS intercalated K4Nb6O17 (designated as K4Nb6O17/PbS) photocatalyst was synthesized via direct ion exchange, alkylamines intercalation and sulfurization procedures. The as-prepared samples were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), ultraviolet-visible diffuse reflection spectra (UV-Vis) and photoluminescence measurement (PL), energy dispersive X-ray detector (EDS) and X-ray fluorescence spectrometer (XRF). The photocatalytic performance of these catalysts for hydrogen production was also investigated in the presence of Na2S and Na2SO3 sacrificial reagents. The absorption edge of K4Nb6O17 shifted to visible light region after the intercalation of PbS. K4Nb6O17/PbS photocatalysts exhibit higher activities for photocatalytic hydrogen production under both UV light and visible light irradiation, and the amounts of hydrogen produced are 123.94 mmol/(g cat) and 0.66 mmol/(g cat) after 3 h irradiation, respectively. The mechanism of charge separation is also discussed.

  5. K5R8 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  6. Differential expression of K4-AP currents and Kv3.1 potassium channel transcripts in cortical neurons that develop distinct firing phenotypes.

    Science.gov (United States)

    Massengill, J L; Smith, M A; Son, D I; O'Dowd, D K

    1997-05-01

    Maturation of electrical excitability during early postnatal development is critical to formation of functional neural circuitry in the mammalian neocortex. Little is known, however, about the changes in gene expression underlying the development of firing properties that characterize different classes of cortical neurons. Here we describe the development of cortical neurons with two distinct firing phenotypes, regular-spiking (RS) and fast-spiking (FS), that appear to emerge from a population of immature multiple-spiking (IMS) neurons during the first two postnatal weeks, both in vivo (within layer IV) and in vitro. We report the expression of a slowly inactivating, 4-AP-sensitive potassium current (K4-AP) at significantly higher density in FS compared with RS neurons. The same current is expressed at intermediate levels in IMS neurons. The kinetic, voltage-dependent, and pharmacological properties of the K4-AP current are similar to those observed by heterologous expression of Kv3.1 potassium channel mRNA. Single-cell RT-PCR analysis demonstrates that PCR products representing Kv3.1 transcripts are amplified more frequently from FS than RS neurons, with an intermediate frequency of Kv3.1 detection in neurons with immature firing properties. Taken together, these data suggest that the Kv3.1 gene encodes the K4-AP current and that expression of this gene is regulated in a cell-specific manner during development. Analysis of the effects of 4-AP on firing properties suggests that the K4-AP current is important for rapid action potential repolarization, fast after-hyperpolarization, brief refractory period, and high firing frequency characteristic of FS GABAergic interneurons.

  7. Histone H3 Acetyl K9 and Histone H3 Tri Methyl K4 as Prognostic Markers for Patients with Cervical Cancer

    Directory of Open Access Journals (Sweden)

    Susanne Beyer

    2017-02-01

    Full Text Available Chromatin remodeling alters gene expression in carcinoma tissue. Although cervical cancer is the fourth most common cancer in women worldwide, a systematic study about the prognostic value of specific changes in the chromatin structure, such as histone acetylation or histone methylation, is missing. In this study, the expression of histone H3 acetyl K9, which is known to denote active regions at enhancers and promoters, and histone H3 tri methyl K4, which preferentially identifies active gene promoters, were examined as both show high metastatic potential. A panel of patients with cervical cancer was selected and the importance of the histone modifications concerning survival-time (overall survival and relapse-free survival was analyzed in 250 cases. Histone H3 acetyl K9 staining was correlated with low grading, low FIGO (TNM classification and the International Federation of Gynecology and Obstetrics status, negative N-status and low T-status in cervical cancer, showing a higher expression in adenocarcinoma than in squamous cell carcinoma. Cytoplasmic expression of histone H3 tri methyl K4 in a cervical cancer specimen was correlated with advanced T-status and poor prognosis. While cytoplasmic H3K4me3 expression seemed to be a marker of relapse-free survival, nuclear expression showed a correlation to poor prognosis in overall survival. Within this study, we analyzed the chemical modification of two histone proteins that are connected to active gene expression. Histone H3 acetyl K9 was found to be an independent marker of overall survival. Histone H3 tri methyl K4 was correlated with poor prognosis and it was found to be an independent marker of relapse-free survival. Therefore, we could show that chromatin remodeling plays an important role in cervical cancer biology.

  8. Histone H3 Acetyl K9 and Histone H3 Tri Methyl K4 as Prognostic Markers for Patients with Cervical Cancer.

    Science.gov (United States)

    Beyer, Susanne; Zhu, Junyan; Mayr, Doris; Kuhn, Christina; Schulze, Sandra; Hofmann, Simone; Dannecker, Christian; Jeschke, Udo; Kost, Bernd P

    2017-02-23

    Chromatin remodeling alters gene expression in carcinoma tissue. Although cervical cancer is the fourth most common cancer in women worldwide, a systematic study about the prognostic value of specific changes in the chromatin structure, such as histone acetylation or histone methylation, is missing. In this study, the expression of histone H3 acetyl K9, which is known to denote active regions at enhancers and promoters, and histone H3 tri methyl K4, which preferentially identifies active gene promoters, were examined as both show high metastatic potential. A panel of patients with cervical cancer was selected and the importance of the histone modifications concerning survival-time (overall survival and relapse-free survival) was analyzed in 250 cases. Histone H3 acetyl K9 staining was correlated with low grading, low FIGO (TNM classification and the International Federation of Gynecology and Obstetrics) status, negative N-status and low T-status in cervical cancer, showing a higher expression in adenocarcinoma than in squamous cell carcinoma. Cytoplasmic expression of histone H3 tri methyl K4 in a cervical cancer specimen was correlated with advanced T-status and poor prognosis. While cytoplasmic H3K4me3 expression seemed to be a marker of relapse-free survival, nuclear expression showed a correlation to poor prognosis in overall survival. Within this study, we analyzed the chemical modification of two histone proteins that are connected to active gene expression. Histone H3 acetyl K9 was found to be an independent marker of overall survival. Histone H3 tri methyl K4 was correlated with poor prognosis and it was found to be an independent marker of relapse-free survival. Therefore, we could show that chromatin remodeling plays an important role in cervical cancer biology.

  9. Characterisation of the effects of ATPA, a GLU(K5) kainate receptor agonist, on GABAergic synaptic transmission in the CA1 region of rat hippocampal slices.

    Science.gov (United States)

    Clarke, V R J; Collingridge, G L

    2004-09-01

    Kainate receptors are implicated in a variety of physiological and pathological processes in the CNS. Previously we demonstrated that (RS)-2-amino-3-(3-hydroxy-5-tert-butylisoxazol-4-yl)propanoic acid (ATPA), a selective agonist for the GLU(K5) subtype of kainate receptor, depresses monosynaptically evoked inhibitory postsynaptic potentials (IPSPs) in the CA1 region of the rat hippocampus. In the current study, we provide a more detailed characterisation of this effect. Firstly, our data demonstrate a rank order of potency of domoate>kainate>ATPA>alpha-amino-3-(3-hydroxy-5-methyl-4-isoxalolyl)propionic acid Secondly, we confirm that the effects of ATPA are not mediated indirectly via the activation of gamma-aminobutyric acid receptors (i.e. either GABA(A) or GABA(B)). Thirdly, we show that the small increase in conductance induced by ATPA is insufficient to account for the depression of monosynaptic inhibition. Fourthly, we show that the effects of ATPA on IPSPs are antagonised by the GLU(K5)-selective antagonist (3S, 4aR, 6S, 8aR)-6-(4-carboxyphenyl)methyl-1,2,3,4,4a,5,6,7,8,8a-decahydroisoquinoline-3-carboxylic acid (LY382884). However, LY382884 is less potent as an antagonist of the effects of ATPA on IPSPs compared to its depressant effect on EPSPs.

  10. Inflammatory genes TNFα and IL6 display no signs of increased H3K4me3 in circulating monocytes from untreated rheumatoid arthritis patients.

    Science.gov (United States)

    Messemaker, T C; Mikkers, H M M; Huizinga, T W; Toes, R E M; van der Helm-van Mil, A H M; Kurreeman, F

    2017-09-01

    Innate immune cells, such as monocytes, can adopt a long-lasting pro-inflammatory phenotype, a phenomenon called 'trained immunity'. In trained immunity, increased cytokine levels of genes, like interleukin (IL)-6 and tumor necrosis factor (TNF)-α, are observed, which are associated with increased histone 3 lysine 4 trimethylation (H3K4me3) in the promoter region. As systemic IL6 and TNFα levels are increased in rheumatoid arthritis (RA) patients and monocytes are known to be the primary producers of TNFα and IL6, we hypothesized that 'trained immunity' signals may be observed at these genes in monocytes from RA patients. CD14+ monocytes were isolated from untreated RA patients and paired age-matched healthy controls. H3K4me3, mRNA, protein and serum levels of IL6 and TNFα were evaluated by chromatin immunoprecipitation, reverse-transcription quantitative PCR and enzyme-linked immunosorbent assays. Despite elevated serum levels of TNFα and IL6 in the tested RA patients (P<0.05), ex vivo isolated monocytes displayed similar H3K4me3 levels to healthy controls in the promoter region of TNFα and IL6. Concordantly, mRNA and protein levels of IL6 and TNFα were similar before and after lipopolysaccharide stimulation between patients and controls. Together, with the current number of individuals tested we have not detected enhanced trained immunity signals in circulating monocytes from untreated RA patients, despite increased IL6 and TNFα serum levels.

  11. Pro Isomerization in MLL1 PHD3-Bromo Cassette Connects H3K4me Readout to CyP33 and HDAC-Mediated Repression

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Zhanxin; Song, Jikui; Milne, Thomas A.; Wang, Gang G.; Li, Haitao; Allis, C. David; Patel, Dinshaw J. (MSKCC); (Rockefeller)

    2010-09-13

    The MLL1 gene is a frequent target for recurrent chromosomal translocations, resulting in transformation of hematopoietic precursors into leukemia stem cells. Here, we report on structure-function studies that elucidate molecular events in MLL1 binding of histone H3K4me3/2 marks and recruitment of the cyclophilin CyP33. CyP33 contains a PPIase and a RRM domain and regulates MLL1 function through HDAC recruitment. We find that the PPIase domain of CyP33 regulates the conformation of MLL1 through proline isomerization within the PHD3-Bromo linker, thereby disrupting the PHD3-Bromo interface and facilitating binding of the MLL1-PHD3 domain to the CyP33-RRM domain. H3K4me3/2 and CyP33-RRM target different surfaces of MLL1-PHD3 and can bind simultaneously to form a ternary complex. Furthermore, the MLL1-CyP33 interaction is required for repression of HOXA9 and HOXC8 genes in vivo. Our results highlight the role of PHD3-Bromo cassette as a regulatory platform, orchestrating MLL1 binding of H3K4me3/2 marks and cyclophilin-mediated repression through HDAC recruitment.

  12. The Demethylase JMJD2C Localizes to H3K4me3 Positive Transcription Start Sites and Is Dispensable for Embryonic Development

    DEFF Research Database (Denmark)

    Pedersen, Marianne Terndrup; Agger, Karl; Laugesen, Anne

    2014-01-01

    cell (ESC) self-renewal and embryonic development. Moreover, we report that JMJD2C localizes to H3K4me3 positive transcription start sites in both primary cells and in the human carcinoma KYSE150 cell line, containing an amplification of the JMJD2C locus. Binding is dependent on the double Tudor domain...... of JMJD2C, which recognizes H3K4me3, but not H4K20me2/me3 in vitro, thus showing a different binding specificity than the double Tudor domains of JMJD2A and JMJD2B. Depletion of JMJD2C in KYSE150 cells has modest impact on H3K9me3 and H3K36me3 levels, but impairs proliferation and leads to deregulated...... expression of a subset of target genes involved in cell cycle progression. Taken together, we show that JMJD2C is targeted to H3K4me3 positive transcription start sites, where it can contribute to transcriptional regulation, and report that the putative oncogene, JMJD2C, is not generally required...

  13. Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axes.

    Science.gov (United States)

    Sommermeyer, Vérane; Béneut, Claire; Chaplais, Emmanuel; Serrentino, Maria Elisabetta; Borde, Valérie

    2013-01-10

    Meiotic chromosomes are organized into arrays of loops that are anchored to the chromosome axis structure. Programmed DNA double-strand breaks (DSBs) that initiate meiotic recombination, catalyzed by Spo11 and accessory DSB proteins, form in loop sequences in promoters, whereas the DSB proteins are located on chromosome axes. Mechanisms bridging these two chromosomal regions for DSB formation have remained elusive. Here we show that Spp1, a conserved member of the histone H3K4 methyltransferase Set1 complex, is required for normal levels of DSB formation and is associated with chromosome axes during meiosis, where it physically interacts with the Mer2 DSB protein. The PHD finger module of Spp1, which reads H3K4 methylation close to promoters, promotes DSB formation by tethering these regions to chromosome axes and activating cleavage by the DSB proteins. This paper provides the molecular mechanism linking DSB sequences to chromosome axes and explains why H3K4 methylation is important for meiotic recombination.

  14. The histone-H3K4-specific demethylase KDM5B binds to its substrate and product through distinct PHD fingers.

    Science.gov (United States)

    Klein, Brianna J; Piao, Lianhua; Xi, Yuanxin; Rincon-Arano, Hector; Rothbart, Scott B; Peng, Danni; Wen, Hong; Larson, Connie; Zhang, Xi; Zheng, Xia; Cortazar, Michael A; Peña, Pedro V; Mangan, Anthony; Bentley, David L; Strahl, Brian D; Groudine, Mark; Li, Wei; Shi, Xiaobing; Kutateladze, Tatiana G

    2014-01-30

    The histone lysine demethylase KDM5B regulates gene transcription and cell differentiation and is implicated in carcinogenesis. It contains multiple conserved chromatin-associated domains, including three PHD fingers of unknown function. Here, we show that the first and third, but not the second, PHD fingers of KDM5B possess histone binding activities. The PHD1 finger is highly specific for unmodified histone H3 (H3K4me0), whereas the PHD3 finger shows preference for the trimethylated histone mark H3K4me3. RNA-seq analysis indicates that KDM5B functions as a transcriptional repressor for genes involved in inflammatory responses, cell proliferation, adhesion, and migration. Biochemical analysis reveals that KDM5B associates with components of the nucleosome remodeling and deacetylase (NuRD) complex and may cooperate with the histone deacetylase 1 (HDAC1) in gene repression. KDM5B is downregulated in triple-negative breast cancer relative to estrogen-receptor-positive breast cancer. Overexpression of KDM5B in the MDA-MB 231 breast cancer cells suppresses cell migration and invasion, and the PHD1-H3K4me0 interaction is essential for inhibiting migration. These findings highlight tumor-suppressive functions of KDM5B in triple-negative breast cancer cells and suggest a multivalent mechanism for KDM5B-mediated transcriptional regulation. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

  15. The Histone-H3K4-Specific Demethylase KDM5B Binds to Its Substrate and Product through Distinct PHD Fingers

    Directory of Open Access Journals (Sweden)

    Brianna J. Klein

    2014-01-01

    Full Text Available The histone lysine demethylase KDM5B regulates gene transcription and cell differentiation and is implicated in carcinogenesis. It contains multiple conserved chromatin-associated domains, including three PHD fingers of unknown function. Here, we show that the first and third, but not the second, PHD fingers of KDM5B possess histone binding activities. The PHD1 finger is highly specific for unmodified histone H3 (H3K4me0, whereas the PHD3 finger shows preference for the trimethylated histone mark H3K4me3. RNA-seq analysis indicates that KDM5B functions as a transcriptional repressor for genes involved in inflammatory responses, cell proliferation, adhesion, and migration. Biochemical analysis reveals that KDM5B associates with components of the nucleosome remodeling and deacetylase (NuRD complex and may cooperate with the histone deacetylase 1 (HDAC1 in gene repression. KDM5B is downregulated in triple-negative breast cancer relative to estrogen-receptor-positive breast cancer. Overexpression of KDM5B in the MDA-MB 231 breast cancer cells suppresses cell migration and invasion, and the PHD1-H3K4me0 interaction is essential for inhibiting migration. These findings highlight tumor-suppressive functions of KDM5B in triple-negative breast cancer cells and suggest a multivalent mechanism for KDM5B-mediated transcriptional regulation.

  16. Epigenetic Profiling of H3K4Me3 Reveals Herbal Medicine Jinfukang-Induced Epigenetic Alteration Is Involved in Anti-Lung Cancer Activity

    Directory of Open Access Journals (Sweden)

    Jun Lu

    2016-01-01

    Full Text Available Traditional Chinese medicine Jinfukang (JFK has been clinically used for treating lung cancer. To examine whether epigenetic modifications are involved in its anticancer activity, we performed a global profiling analysis of H3K4Me3, an epigenomic marker associated with active gene expression, in JFK-treated lung cancer cells. We identified 11,670 genes with significantly altered status of H3K4Me3 modification following JFK treatment (P<0.05. Gene Ontology analysis indicates that these genes are involved in tumor-related pathways, including pathway in cancer, basal cell carcinoma, apoptosis, induction of programmed cell death, regulation of transcription (DNA-templated, intracellular signal transduction, and regulation of peptidase activity. In particular, we found that the levels of H3K4Me3 at the promoters of SUSD2, CCND2, BCL2A1, and TMEM158 are significantly altered in A549, NCI-H1975, NCI-H1650, and NCI-H2228 cells, when treated with JFK. Collectively, these findings provide the first evidence that the anticancer activity of JFK involves modulation of histone modification at many cancer-related gene loci.

  17. Stage and gene specific signatures defined by histones H3K4me2 and H3K27me3 accompany mammalian retina maturation in vivo.

    Science.gov (United States)

    Popova, Evgenya Y; Xu, Xuming; DeWan, Andrew T; Salzberg, Anna C; Berg, Arthur; Hoh, Josephine; Zhang, Samuel S; Barnstable, Colin J

    2012-01-01

    The epigenetic contribution to neurogenesis is largely unknown. There is, however, growing evidence that posttranslational modification of histones is a dynamic process that shows many correlations with gene expression. Here we have followed the genome-wide distribution of two important histone H3 modifications, H3K4me2 and H3K27me3 during late mouse retina development. The retina provides an ideal model for these studies because of its well-characterized structure and development and also the extensive studies of the retinal transcriptome and its development. We found that a group of genes expressed only in mature rod photoreceptors have a unique signature consisting of de-novo accumulation of H3K4me2, both at the transcription start site (TSS) and over the whole gene, that correlates with the increase in transcription, but no accumulation of H3K27me3 at any stage. By in silico analysis of this unique signature we have identified a larger group of genes that may be selectively expressed in mature rod photoreceptors. We also found that the distribution of H3K4me2 and H3K27me3 on the genes widely expressed is not always associated with their transcriptional levels. Different histone signatures for retinal genes with the same gene expression pattern suggest the diversities of epigenetic regulation. Genes without H3K4me2 and H3K27me3 accumulation at any stage represent a large group of transcripts never expressed in retina. The epigenetic signatures defined by H3K4me2 and H3K27me3 can distinguish cell-type specific genes from widespread transcripts and may be reflective of cell specificity during retina maturation. In addition to the developmental patterns seen in wild type retina, the dramatic changes of histone modification in the retinas of mutant animals lacking rod photoreceptors provide a tool to study the epigenetic changes in other cell types and thus describe a broad range of epigenetic events in a solid tissue in vivo.

  18. Stage and gene specific signatures defined by histones H3K4me2 and H3K27me3 accompany mammalian retina maturation in vivo.

    Directory of Open Access Journals (Sweden)

    Evgenya Y Popova

    Full Text Available The epigenetic contribution to neurogenesis is largely unknown. There is, however, growing evidence that posttranslational modification of histones is a dynamic process that shows many correlations with gene expression. Here we have followed the genome-wide distribution of two important histone H3 modifications, H3K4me2 and H3K27me3 during late mouse retina development. The retina provides an ideal model for these studies because of its well-characterized structure and development and also the extensive studies of the retinal transcriptome and its development. We found that a group of genes expressed only in mature rod photoreceptors have a unique signature consisting of de-novo accumulation of H3K4me2, both at the transcription start site (TSS and over the whole gene, that correlates with the increase in transcription, but no accumulation of H3K27me3 at any stage. By in silico analysis of this unique signature we have identified a larger group of genes that may be selectively expressed in mature rod photoreceptors. We also found that the distribution of H3K4me2 and H3K27me3 on the genes widely expressed is not always associated with their transcriptional levels. Different histone signatures for retinal genes with the same gene expression pattern suggest the diversities of epigenetic regulation. Genes without H3K4me2 and H3K27me3 accumulation at any stage represent a large group of transcripts never expressed in retina. The epigenetic signatures defined by H3K4me2 and H3K27me3 can distinguish cell-type specific genes from widespread transcripts and may be reflective of cell specificity during retina maturation. In addition to the developmental patterns seen in wild type retina, the dramatic changes of histone modification in the retinas of mutant animals lacking rod photoreceptors provide a tool to study the epigenetic changes in other cell types and thus describe a broad range of epigenetic events in a solid tissue in vivo.

  19. Hypersensitisation using 266nm Laser Light

    DEFF Research Database (Denmark)

    Sørensen, Henrik Rokkjær; Canning, John; Kristensen, Martin

    UV-hypersensitisation using 266nm VW-light on hydrogenated Ge-doped fibre is reported. The optimum fluence to be between 5 to 10 kJ/cm2, coinciding with results obtained using 355nm light, indicating same end-process in both reactions.......UV-hypersensitisation using 266nm VW-light on hydrogenated Ge-doped fibre is reported. The optimum fluence to be between 5 to 10 kJ/cm2, coinciding with results obtained using 355nm light, indicating same end-process in both reactions....

  20. Hypersensitisation using 266nm Laser Light

    DEFF Research Database (Denmark)

    Sørensen, Henrik Rokkjær; Canning, John; Kristensen, Martin

    UV-hypersensitisation using 266nm VW-light on hydrogenated Ge-doped fibre is reported. The optimum fluence to be between 5 to 10 kJ/cm2, coinciding with results obtained using 355nm light, indicating same end-process in both reactions.......UV-hypersensitisation using 266nm VW-light on hydrogenated Ge-doped fibre is reported. The optimum fluence to be between 5 to 10 kJ/cm2, coinciding with results obtained using 355nm light, indicating same end-process in both reactions....

  1. Lithography strategy for 65-nm node

    Science.gov (United States)

    Borodovsky, Yan A.; Schenker, Richard E.; Allen, Gary A.; Tejnil, Edita; Hwang, David H.; Lo, Fu-Chang; Singh, Vivek K.; Gleason, Robert E.; Brandenburg, Joseph E.; Bigwood, Robert M.

    2002-07-01

    Intel will start high volume manufacturing (HVM) of the 65nm node in 2005. Microprocessor density and performance trends will continue to follow Moore's law and cost-effective patterning solutions capable of supporting it have to be found, demonstrated and developed during 2002-2004. Given the uncertainty regarding the readiness and respective capabilities of 157nm and 193nm lithography to support 65nm technology requirements, Intel is developing both lithographic options and corresponding infrastructure with the intent to use both options in manufacturing. Development and use of dual lithographic options for a given technology node in manufacturing is not a new paradigm for Intel: whenever introduction of a new exposure wavelength presented excessive risk to the manufacturing schedule, Intel developed parallel patterning approaches in time for the manufacturing ramp. Both I-line and 248nm patterning solutions were developed and successfully used in manufacturing of the 350nm node at Intel. Similarly, 248nm and 193nm patterning solutions were fully developed for 130nm node high volume manufacturing.

  2. Advances in 750 nm VECSELs (Conference Presentation)

    Science.gov (United States)

    Saarinen, Esa J.; Ranta, Sanna; Lyytikäinen, Jari; Saarela, Antti; Sirbu, Alexei; Iakovlev, Vladimir; Kapon, Eli; Guina, Mircea

    2017-03-01

    Lasers operating in the transmission window of tissue at wavelengths between 700 and 800 nm are needed in numerous medical and biomedical applications, including photodynamic therapy and fluorescence microscopy. However, the performance of diode lasers in this spectral range is limited by the lack of appropriate compound semiconductors. Here, we review our recent research on 750 nm VECSELs. Two approaches to reaching the 750 nm wavelength will be discussed. The first approach relies on intra-cavity frequency doubling a wafer-fused 1500 nm VECSEL. The VECSEL gain chip comprises a GaAs-based DBR and an InP-based gain section, which allows for optical pumping with low-cost commercial diodes at 980 nm. With this scheme we have achieved watt-level output powers and tuning of the laser wavelength over a 40 nm band at around 750 nm. The second approach is direct emission at 750 nm using the AlGaAs/GaAs material system. In this approach visible wavelengths are required for optical pumping. However, the consequent higher costs compared to pumping at 980 nm are mitigated by the more compact laser setup and prospects of doubling the frequency to the ultraviolet range.

  3. Dust Explosion Characteristics of Agglomerated 35 nm and 100 nm Aluminum Particles

    Directory of Open Access Journals (Sweden)

    Hong-Chun Wu

    2010-01-01

    Full Text Available In the experiment, nanoparticles of 35 nm Al and 100 nm Al powders, respectively, formed particles with average sizes of 161 nm and 167 nm in agglomeration. The characteristics of dust cloud explosions with the two powder sizes, 35 nm and 100 nm, revealed considerable differences, as shown here: (dp/dtmax-35 nm = 1254 bar/s, (dp/dtmax-100 nm = 1105 bar/s; Pmax-35 nm = 7.5 bar, Pmax-100 nm = 12.3 bar, and MEC-35 nm = 40 g/m3, MEC-100 nm = 50 g/m3. The reason of Pmax-35 nm value is smaller than Pmax-100 nm may be due to agglomeration. From an analysis of the explosive residue, the study found that nanoparticles of 35 nm Al powder became filamentous strands after an explosion, where most of 100 nm Al nanoparticles maintained a spherical structure, This may be because the initial melting temperature of 35 nm Al is 435.71°C, while that for 100 nm Al is 523.58°C, higher by 87.87°C. This study discovered that explosive property between the 35 nm Al and 100 nm Al powders after agglomeration were different.

  4. Laser hypersensitisation using 266nm light

    DEFF Research Database (Denmark)

    Sørensen, Henrik Rokkjær; Canning, J.; Kristensen, Martin

    2005-01-01

    UV hypersensitisation using CW 266 nm light on hydrogenated Ge-doped fibre is reported. The optimum sensitisation fluence is found to be in the range of 5 to 10 kJ/cm2, coinciding with previous results obtained using 355 nm light, indicating the same end-process used in the photochemical reaction...

  5. The Application of Red Pigments from Streptomyces K-4B and Dayak Onions (Eleutherine palmifolia (L.) Merr.) In Colouring Glycerine Soap

    Science.gov (United States)

    Herlina; Asnani, A.; Diastuti, H.

    2017-02-01

    Glycerin soap has been colored with red pigment from Streptomyces K-4 B and Dayak onion (Eleutherine palmifolia (L.) Merr). Both red pigments from Streptomyces K-4B and Dayak onion were extracted with ethanol by maceration method, followed with soxhlet extraction. The concentration of red pigment added was varied (0, 200, 300, 400 μL) to evaluate the best product. The resulted glycerine soaps were characterized and analyzed based on SNI 06-3532-1994. The research results indicated that the glycerine soap has water content ranged from 0.36% to 12.56%; the amount of fatty acid ranged from 14% to 36.75%; the amount of free fatty acids ranged from 0% to 0.37%; the non-saponifiable fat ranged from 0.001 to 0.019%; the pH ranged from 10.33 to 11.06; the foam stability ranged from 0.61% to 89.09%. The results of analysis of variance showed that the effect between treatments significantly different at 95% confidence level (α = 0.05) on the characteristics of glycerine soap. The results of an organoleptic test with parameters observed were color, aroma, texture, foam, rough impression upon usage and rough impression after usage, gave “like to very like soap” with a maximum score of 4.67 (1 to 5 scale). Based on the color assessment, the organoleptic panelists preferred the glycerine soap of SK-4B3 (red pigment from Streptomyces K-4B, 200 μL) with the score of 4.30 (like to very like).

  6. Ag 插层 K4Nb6O17复合光催化剂降解水中有机污染物%The Degradation of Organic Pollutants in Water on The Ag-intercalated K4Nb6O17 Composite Photocatalyst

    Institute of Scientific and Technical Information of China (English)

    穆晨帆; 梁英华; 刘利; 胡金山; 崔文权

    2015-01-01

    Ag-intercalated layered niobate (denoted as K4Nb6O17/Ag) was synthesized via a micr-owave-assisted ion-exchange method. The high enhanced activity of the composite photocatalyst was observed during the degradation of methylene blue (MB). Also, the degradation rate of MB by K4Nb6O17/Ag was higher when the solution pH increased. The composite photocatalyst showed the high enhanced activity with the different concentration of MB. In the same condition, K4Nb6O17/Ag showed higher photocatalysis than Ag-loaded K4Nb6O17 and Ag-loaded TiO2 composite photocat-alysts.%介绍了通过微波辅助的离子交换法制备 Ag 插层 K4Nb6O17复合光催化剂。复合光催化剂在降解水体中亚甲基蓝的过程中呈现出很高的光催化活性,并且插层复合光催化剂对亚基蓝在碱性条件下能发挥更高效的降解作用且对不同初始浓度的亚甲基蓝均具有较好的移除降解效率。在相同的反应条件下, Ag 插层复合光催化剂要比等量的 Ag 负载 K4Nb6O17, Ag 负载 TiO2复合光催化剂活性高很多,使 Ag 插层 K4Nb6O17复合光催化剂在净化水体污染物方面表现出更显著的优势。

  7. Re-patterning of H3K27me3, H3K4me3 and DNA methylation during fibroblast conversion into induced cardiomyocytes

    Directory of Open Access Journals (Sweden)

    Ziqing Liu

    2016-03-01

    Full Text Available Direct conversion of fibroblasts into induced cardiomyocytes (iCMs offers an alternative strategy for cardiac disease modeling and regeneration. During iCM reprogramming, the starting fibroblasts must overcome existing epigenetic barriers to acquire the CM-like chromatin pattern. However, epigenetic dynamics along this reprogramming process have not been studied. Here, we took advantage of our recently generated polycistronic system and determined the dynamics of two critical histone marks, H3K27me3 and H3K4me3, in parallel with gene expression at a set of carefully selected cardiac and fibroblast loci during iCM reprogramming. We observed reduced H3K27me3 and increased H3K4me3 at cardiac promoters as early as day 3, paralleled by a rapid significant increase in their mRNA expression. In contrast, H3K27me3 at loci encoding fibroblast marker genes did not increase until day 10 and H3K4me3 progressively decreased along the reprogramming process; these changes were accompanied by a gradual decrease in the mRNA expression of fibroblast marker genes. Further analyses of fibroblast-enriched transcription factors revealed a similarly late deposition of H3K27me3 and decreased mRNA expression of Sox9, Twist1 and Twist2, three important players in epithelial−mesenchymal transition. Our data suggest early rapid activation of the cardiac program and later progressive suppression of fibroblast fate at both epigenetic and transcriptional levels. Additionally, we determined the DNA methylation states of representative cardiac promoters and found that not every single CpG was equally demethylated during early stages of iCM reprogramming. Rather, there are specific CpGs, whose demethylation states correlated tightly with transcription activation, that we propose are the major contributing CpGs. Our work thus reveals a differential re-patterning of H3K27me3, H3K4me3 at cardiac and fibroblast loci during iCM reprogramming and could provide future genome

  8. Final report on the torque comparison EURAMET.M.T-S2, measurand torque: 10 N.m, 20 N.m, 40 N.m, 60 N.m, 80 N.m, and 100 N.m

    Science.gov (United States)

    Röske, Dirk

    2017-01-01

    The purpose of the EURAMET comparison EURAMET.M.T-S2 was to compare the measuring capabilities up to 100 N.m of a reference-type torque calibration machine of ZAG, Slovenia, with the torque standard machine of the Physikalisch-Technische Bundesanstalt (PTB, Braunschweig, Germany) acting as pilot laboratory. A very stable TT1 torque transducer with well-known properties and two torque measuring bridges was used as travelling standard. According to the technical protocol, torque steps of at least 10 N.m, 20 N.m, 40 N.m, 60 N.m, 80 N.m, and 100 N.m had to be measured both in clockwise and anticlockwise directions. For each of the torque steps and both senses of direction of the torque vector, En values were calculated. The results are in general in good agreement with the claimed measurement uncertainties except for the very first measurement at ZAG with additional support and four plate couplings. It seems to be sufficient in a vertical set-up (vertical torque axis) to use only two flexible couplings and there is no need for a further support between the transducers. The measurements with two couplings fulfill the requirement to the En value and support ZAG's claimed uncertainties of measurement. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  9. Studies on nanosecond 532nm and 355nm and ultrafast 515nm and 532nm laser cutting super-hard materials

    Science.gov (United States)

    Zhang, Jie; Tao, Sha; Wang, Brian; Zhao, Jay

    2017-02-01

    In this paper, micro-processing of three kinds of super-hard materials of poly-crystal diamond (PCD)/tungsten-carbide (WC), CVD-diamond and cubic boron nitride (CNB) has been systematically studied using nanosecond laser (532nm and 355nm), and ultrafast laser (532nm and 515nm). Our purpose is to investigate a full laser micro-cutting solution to achieve a ready-to-use cutting tool insert (CTI). The results show a clean cut with little burns and recasting at edge. The cutting speed of 2-10mm/min depending on thickness was obtained. The laser ablation process was also studied by varying laser parameters (wavelength, pulse width, pulse energy, repetition rate) and tool path to improve cutting speed. Also, studies on material removal efficiency (MRE) of PCD/WC with 355nm-ns and 515nm-fs laser as a function of laser fluence show that 355nm-ns laser is able to achieve higher MRE for PCD and WC. Thus, ultrafast laser is not necessarily used for superhard material cutting. Instead, post-polishing with ultrafast laser can be used to clean cutting surface and improve smoothness.

  10. Laser Damage Growth in Fused Silica with Simultaneous 351 nm and 1053 nm irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Norton, M A; Carr, A V; Carr, C W; Donohue, E E; Feit, M D; Hollingsworth, W G; Liao, Z; Negres, R A; Rubenchik, A M; Wegner, P J

    2008-10-24

    Laser-induced growth of optical damage often determines the useful lifetime of an optic in a high power laser system. We have extended our previous work on growth of laser damage in fused silica with simultaneous 351 nm and 1053 nm laser irradiation by measuring the threshold for growth with various ratios of 351 nm and 1053 nm fluence. Previously we reported that when growth occurs, the growth rate is determined by the total fluence. We now find that the threshold for growth is dependent on both the magnitude of the 351 nm fluence as well as the ratio of the 351 nm fluence to the 1053 nm fluence. Furthermore, the data suggests that under certain conditions the 1053 nm fluence does not contribute to the growth.

  11. Electrochemical studies on Li+/K+ ion exchange behaviour in K4Fe(CN)6 cathode material for Li, K-ion battery

    Indian Academy of Sciences (India)

    Bikash Mandal; I Basumallick; Susanta Ghosha

    2015-01-01

    The electrochemical studies of anhydrous K4Fe(CN)6 is reported. Anhydrous material was produced after dehydrating K4Fe(CN)6.3H2O crystal at 200°C in an open atmosphere. The material, as obtained, was characterized by various spectroscopic techniques, such as UV-Visible, FTIR, powder X-ray diffraction and FESEM-EDX. Electrochemical and Li+/K+ ion exchange behaviour of the synthesized material were studied by cyclic voltametry (CV), chronoamperometry (CA) and galvanostatic charge-discharge method after preparing a laboratory model cell against lithium anode instead of potassium. During anodic scan in the 1st cycle, peak maximum was observed at 3.93 V vs. Li+/Li due to removal of K+ ions from the ferrocyanide matrix, whereas, in the reverse scan (cathodic sweep) as well as in consequent cycles, peak maxima due to Li+ ion insertion and extraction were observed at 2.46 V and 3.23 V vs. Li+/Li, respectively. Cell, assembled using ferrocyanide cathode and lithium anode, shows an open circuit potential of 3.08 V and delivers a maximum capacity of 61 mAh g-1 (theoretical capacity 72 mAh g-1) at a rate of 0.2 C at room temperature.

  12. The inflammatory kinase MAP4K4 promotes reactivation of Kaposi's sarcoma herpesvirus and enhances the invasiveness of infected endothelial cells.

    Directory of Open Access Journals (Sweden)

    Darya A Haas

    Full Text Available Kaposi's sarcoma (KS is a mesenchymal tumour, which is caused by Kaposi's sarcoma herpesvirus (KSHV and develops under inflammatory conditions. KSHV-infected endothelial spindle cells, the neoplastic cells in KS, show increased invasiveness, attributed to the elevated expression of metalloproteinases (MMPs and cyclooxygenase-2 (COX-2. The majority of these spindle cells harbour latent KSHV genomes, while a minority undergoes lytic reactivation with subsequent production of new virions and viral or cellular chemo- and cytokines, which may promote tumour invasion and dissemination. In order to better understand KSHV pathogenesis, we investigated cellular mechanisms underlying the lytic reactivation of KSHV. Using a combination of small molecule library screening and siRNA silencing we found a STE20 kinase family member, MAP4K4, to be involved in KSHV reactivation from latency and to contribute to the invasive phenotype of KSHV-infected endothelial cells by regulating COX-2, MMP-7, and MMP-13 expression. This kinase is also highly expressed in KS spindle cells in vivo. These findings suggest that MAP4K4, a known mediator of inflammation, is involved in KS aetiology by regulating KSHV lytic reactivation, expression of MMPs and COX-2, and, thereby modulating invasiveness of KSHV-infected endothelial cells.

  13. Signalling couples hair follicle stem cell quiescence with reduced histone H3 K4/K9/K27me3 for proper tissue homeostasis.

    Science.gov (United States)

    Lee, Jayhun; Kang, Sangjo; Lilja, Karin C; Colletier, Keegan J; Scheitz, Cornelia Johanna Franziska; Zhang, Ying V; Tumbar, Tudorita

    2016-04-15

    Mechanisms of plasticity to acquire different cell fates are critical for adult stem cell (SC) potential, yet are poorly understood. Reduced global histone methylation is an epigenetic state known to mediate plasticity in cultured embryonic SCs and T-cell progenitors. Here we find histone H3 K4/K9/K27me3 levels actively reduced in adult mouse skin and hair follicle stem cells (HFSCs) during G0 quiescence. The level of marks over specific gene promoters did not correlate to mRNA level changes in quiescent HFSCs. Skin hypomethylation during quiescence was necessary for subsequent progression of hair homeostasis (cycle). Inhibiting BMP signal, a known HFSC anti-proliferative factor, elevated HFSC methylation in vivo during quiescence prior to proliferation onset. Furthermore, removal of proliferation factors and addition of BMP4 reduced histone methylases and increased demethylases mRNAs in cultured skin epithelial cells. We conclude that signalling couples hair follicle stem cell quiescence with reduced H3 K4/K9/K27me3 levels for proper tissue homeostasis.

  14. H3K4me3 demethylation by the histone demethylase KDM5C/JARID1C promotes DNA replication origin firing.

    Science.gov (United States)

    Rondinelli, Beatrice; Schwerer, Hélène; Antonini, Elena; Gaviraghi, Marco; Lupi, Alessio; Frenquelli, Michela; Cittaro, Davide; Segalla, Simona; Lemaitre, Jean-Marc; Tonon, Giovanni

    2015-03-11

    DNA replication is a tightly regulated process that initiates from multiple replication origins and leads to the faithful transmission of the genetic material. For proper DNA replication, the chromatin surrounding origins needs to be remodeled. However, remarkably little is known on which epigenetic changes are required to allow the firing of replication origins. Here, we show that the histone demethylase KDM5C/JARID1C is required for proper DNA replication at early origins. JARID1C dictates the assembly of the pre-initiation complex, driving the binding to chromatin of the pre-initiation proteins CDC45 and PCNA, through the demethylation of the histone mark H3K4me3. Fork activation and histone H4 acetylation, additional early events involved in DNA replication, are not affected by JARID1C downregulation. All together, these data point to a prominent role for JARID1C in a specific phase of DNA replication in mammalian cells, through its demethylase activity on H3K4me3.

  15. Distinct localization of histone H3 acetylation and H3-K4 methylation to the transcription start sites in the human genome

    Science.gov (United States)

    Liang, Gangning; Lin, Joy C. Y.; Wei, Vivian; Yoo, Christine; Cheng, Jonathan C.; Nguyen, Carvell T.; Weisenberger, Daniel J.; Egger, Gerda; Takai, Daiya; Gonzales, Felicidad A.; Jones, Peter A.

    2004-01-01

    Almost 1-2% of the human genome is located within 500 bp of either side of a transcription initiation site, whereas a far larger proportion (≈25%) is potentially transcribable by elongating RNA polymerases. This observation raises the question of how the genome is packaged into chromatin to allow start sites to be recognized by the regulatory machinery at the same time as transcription initiation, but not elongation, is blocked in the 25% of intragenic DNA. We developed a chromatin scanning technique called ChAP, coupling the chromatin immunoprecipitation assay with arbitrarily primed PCR, which allows for the rapid and unbiased comparison of histone modification patterns within the eukaryotic nucleus. Methylated lysine 4 (K4) and acetylated K9/14 of histone H3 were both highly localized to the 5′ regions of transcriptionally active human genes but were greatly decreased downstream of the start sites. Our results suggest that the large transcribed regions of human genes are maintained in a deacetylated conformation in regions read by elongating polymerase. Common models depicting widespread histone acetylation and K4 methylation throughout the transcribed unit do not therefore apply to the majority of human genes. PMID:15123803

  16. Potent and Selective KDM5 Inhibitor Stops Cellular Demethylation of H3K4me3 at Transcription Start Sites and Proliferation of MM1S Myeloma Cells.

    Science.gov (United States)

    Tumber, Anthony; Nuzzi, Andrea; Hookway, Edward S; Hatch, Stephanie B; Velupillai, Srikannathasan; Johansson, Catrine; Kawamura, Akane; Savitsky, Pavel; Yapp, Clarence; Szykowska, Aleksandra; Wu, Na; Bountra, Chas; Strain-Damerell, Claire; Burgess-Brown, Nicola A; Ruda, Gian Filippo; Fedorov, Oleg; Munro, Shonagh; England, Katherine S; Nowak, Radoslaw P; Schofield, Christopher J; La Thangue, Nicholas B; Pawlyn, Charlotte; Davies, Faith; Morgan, Gareth; Athanasou, Nick; Müller, Susanne; Oppermann, Udo; Brennan, Paul E

    2017-03-16

    Methylation of lysine residues on histone tail is a dynamic epigenetic modification that plays a key role in chromatin structure and gene regulation. Members of the KDM5 (also known as JARID1) sub-family are 2-oxoglutarate (2-OG) and Fe(2+)-dependent oxygenases acting as histone 3 lysine 4 trimethyl (H3K4me3) demethylases, regulating proliferation, stem cell self-renewal, and differentiation. Here we present the characterization of KDOAM-25, an inhibitor of KDM5 enzymes. KDOAM-25 shows biochemical half maximal inhibitory concentration values of <100 nM for KDM5A-D in vitro, high selectivity toward other 2-OG oxygenases sub-families, and no off-target activity on a panel of 55 receptors and enzymes. In human cell assay systems, KDOAM-25 has a half maximal effective concentration of ∼50 μM and good selectivity toward other demethylases. KDM5B is overexpressed in multiple myeloma and negatively correlated with the overall survival. Multiple myeloma MM1S cells treated with KDOAM-25 show increased global H3K4 methylation at transcriptional start sites and impaired proliferation. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  17. The histone H3K4-specific demethylase KDM5B binds to its substrate and product through distinct PHD fingers

    Science.gov (United States)

    Klein, Brianna J.; Piao, Lianhua; Xi, Yuanxin; Rincon-Arano, Hector; Rothbart, Scott B.; Peng, Danni; Wen, Hong; Larson, Connie; Zhang, Xi; Zheng, Xia; Cortazar, Michael A.; Peña, Pedro V.; Mangan, Anthony; Bentley, David L.; Strahl, Brian D.; Groudine, Mark; Li, Wei; Shi, Xiaobing; Kutateladze, Tatiana G.

    2014-01-01

    SUMMARY The histone lysine demethylase KDM5B regulates gene transcription and cell differentiation. It contains three PHD fingers, the biological roles of which remain elusive. Here, we show that the first PHD1 finger of KDM5B binds unmodified histone H3, whereas the third PHD3 finger prefers the trimethylated mark, H3K4me3. RNA-seq analysis indicates that KDM5B functions as a transcriptional repressor for a set of genes. Biochemical analysis reveals that KDM5B associates with components of the nucleosome remodeling and deacetylase (NuRD) complex and may cooperate with HDAC1 in gene repression. Compared with the estrogen receptor positive breast cancers, KDM5B is downregulated in the triple-negative breast cancer. Overexpression of KDM5B in the MDA-MB 231 breast cancer cells suppresses cell migration and invasion ability, and the PHD1-H3K4me0 interaction is important for inhibition of migration. These findings highlight tumor-suppressive functions of KDM5B in triple-negative breast cancer cells and suggest a novel multivalent mechanism for KDM5B-mediated transcriptional regulation. PMID:24412361

  18. K4I3 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  19. K4C0 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  20. K4V0 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  1. K4M9 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  2. K4A9 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  3. 130-nm tunable grating-mirror VCSEL

    DEFF Research Database (Denmark)

    Chung, Il-Sug; Mørk, Jesper

    2014-01-01

    We have reported that a combination of the high-index-contrast grating (HCG) mirror as movable mirror and the extended cavity configuration with an antireflection layer can provide a tuning wavelength range of 100 nm for tunable VCSELs. Here, we report that using the air-coupled cavity configurat......We have reported that a combination of the high-index-contrast grating (HCG) mirror as movable mirror and the extended cavity configuration with an antireflection layer can provide a tuning wavelength range of 100 nm for tunable VCSELs. Here, we report that using the air-coupled cavity...... configuration instead of the extended cavity configuration can bring 130-nm tuning range around 1330-nm wavelength. The air-coupled cavity is known to reduce the quantum confinement factor in VCSELs, increasing threshold. In our air-coupled cavity HCG VCSEL case, the very short power penetration length...

  4. Design of an 1800 nm Raman Amplifier

    DEFF Research Database (Denmark)

    Svane, Ask Sebastian; Rottwitt, Karsten

    , also extended band amplifiers are required. As a solution to the latter challenge, Raman amplifiers are suggested as promising candidates. The main hurdle when designing a long wavelength Raman amplifier is the increased intrinsic fiber attenuation which as a consequence leads to an increase...... in the pump power requirement and deteriorated noise properties. Here we demonstrate a Raman amplifier designed for signal wavelengths around 1800 nm. The amplification fiber is an OFS PM Raman fiber, and is pumped by a Raman fiber laser emitting at 1680 nm [4]. The amplifier was pumped co......-polarized and backward, with respect to the singal. In Fig. 2 a measured Raman on/off gain exceeding 9 dB for 285 mW of injected pump power is obtained in a 4.35 km long fiber. A broadband supercontinuum source was used as a signal from 1700 nm to 1900 nm....

  5. Laser hypersensitisation using 266nm light

    DEFF Research Database (Denmark)

    Sørensen, Henrik Rokkjær; Canning, J.; Kristensen, Martin

    2005-01-01

    UV hypersensitisation using CW 266 nm light on hydrogenated Ge-doped fibre is reported. The optimum sensitisation fluence is found to be in the range of 5 to 10 kJ/cm2, coinciding with previous results obtained using 355 nm light, indicating the same end-process used in the photochemical reaction....... We also report the observation of type IA behaviour using this wavelength....

  6. Cascaded quadratic soliton compression at 800 nm

    DEFF Research Database (Denmark)

    Bache, Morten; Bang, Ole; Moses, Jeffrey;

    2007-01-01

    We study soliton compression in quadratic nonlinear materials at 800 nm, where group-velocity mismatch dominates. We develop a nonlocal theory showing that efficient compression depends strongly on characteristic nonlocal time scales related to pulse dispersion.......We study soliton compression in quadratic nonlinear materials at 800 nm, where group-velocity mismatch dominates. We develop a nonlocal theory showing that efficient compression depends strongly on characteristic nonlocal time scales related to pulse dispersion....

  7. A novel 852-nm tunable fiber laser

    Institute of Scientific and Technical Information of China (English)

    Yanlong Shen; Chun Gu; LixinXu; Anting Wang; Hai Ming; Yang Liu; Xiaobing Wang

    2009-01-01

    @@ We report a novel fiber laser operating at 850-nm band by using semiconductor optical amplifier and fiber grating.The laser system is stable, compact, and the operating wavelength can be tuned continuously from about 851 to 854 nm for Cs atomic clock system by stretching the fiber grating.An output power up to 20 mW is obtained with a signal-to-background ratio beyond 30 dB.

  8. PM Raman fiber laser at 1679 nm

    DEFF Research Database (Denmark)

    Svane, Ask Sebastian; Rottwitt, Karsten

    2012-01-01

    We demonstrate a PM Raman fiber laser emitting light at 1679 nm. The laser has an slope efficiency of 67 % and an output power of more than 275mWwith a 27 pm linewidth.......We demonstrate a PM Raman fiber laser emitting light at 1679 nm. The laser has an slope efficiency of 67 % and an output power of more than 275mWwith a 27 pm linewidth....

  9. Effects of histone acetylation and H3K4 methylation on PRA/PRB in human uterine smooth muscle cells during pregnancy%组蛋白H3、H4乙酰化及H3K4甲基化对人妊娠子宫平滑肌细胞PRA/PRB的影响

    Institute of Scientific and Technical Information of China (English)

    罗慧; 陈诚; 梁志清

    2014-01-01

    目的 通过组蛋白去乙酰化酶抑制剂(trichostatin A,TSA)、H3 K4甲基化酶抑制剂(5’-deoxy-5’-methylthioadenosine,MTA)处理人妊娠子宫平滑肌细胞,干预组蛋白H3、H4乙酰化及H3K4甲基化水平,探讨组蛋白H3、H4乙酰化及H3K4甲基化对人妊娠子宫平滑肌细胞PRA/PRB的影响.方法 分离纯化人妊娠子宫平滑肌细胞(n=16),免疫组化定位孕激素受体(progesterone receptor,PR)及孕激素受体B(progesterone receptor B,PRB)在子宫平滑肌细胞核的表达.分别利用不同浓度TSA、MTA对其进行处理,Real-time PCR检测PR、PRA、PRB mRNA的表达;染色质免疫共沉淀技术(Chromatin immunoprecipitation,ChIP)比较处理前后PRA、PRB启动子区H3、H4乙酰化及H3K4三甲基化水平.结果 TSA可使PRA/PRB明显增高(P<0.05),使PRA启动子区H3、H4乙酰化水平明显上升(P<0.05).MTA可使PRA/PRB明显下降(P<0.05),PRA启动子区H3K4乙酰化水平明显下降(P<0.05).两种药物的干预主要通过对PRA的调控来调节PRA/PRB比值.结论 组蛋白H3、H4乙酰化和H3K4甲基化均可使人妊娠子宫平滑肌细胞PRA/PRB比值发生改变,可能参与“功能性孕激素撤退”机制的调节.

  10. MicroRNA-25通过调控MAP2 K4抑制糖尿病肾病纤维化的研究%Study of Inhibition Fibrosis of MicroRNA-25 on Diabetic Nephropathy by Regulating Expression of MAP2 K4

    Institute of Scientific and Technical Information of China (English)

    王晓莉; 刘洁婷; 张春雷; 王超男; 冯彪; 初彦辉; 张涛

    2015-01-01

    Objective To determine the expression level of microRNA-25 in animal models of diabetic nephropathy and human renal tubular epithelial cells (HK-2) cultured in different conditions, and to explore its regulating effect on the fibrosis in diabetic nephropathy. Methods The expression of microRNA-25 was detected by real-time PCR. The downstream target protein of microRNA-25 was verified by bioinformatic prediction, transient transfection of cells and Western blotting. Results MicroRNA-25 was down-regulated in animal models of diabetic nephropathy and HK-2 cells which were cultured in high-glucose medium (P<0. 01). MAP2K4 might be the downstream target protein of microRNA-25. Overexpression of microRNA-25 reduced the protein expression of MAP2K4 and α-SMA (P<0. 01). Conclusion MicroRNA-25 inhibits the fibrosis in diabetic nephropathy by regulating the expression of MAP2K4.%目的:检测microRNA-25在糖尿病肾病动物模型及不同条件培养下的人肾小管上皮细胞( HK-2)中的表达,探讨其对糖尿病肾病纤维化的调节作用。方法实时荧光定量聚合酶链反应检测microRNA-25的表达。生物信息学预测、细胞瞬时转染和Western blot验证microRNA-25的下游靶蛋白。结果 microRNA-25在糖尿病肾病动物模型及高糖培养下的HK-2中表达降低(P<0.01)。 MAP2K4可能是microRNA-25的下游靶蛋白。过表达microRNA-25可以从蛋白水平上抑制MAP2K4、α-SMA的表达(P<0.01)。结论 microRNA-25可以通过调控MAP2K4从而抑制糖尿病肾病纤维化的进展。

  11. Final report of key comparison AFRIMETS.AUV.A-K5: primary pressure calibration of LS1P microphones according to IEC 61094-2, over the frequency range 2 Hz to 10 kHz.

    Science.gov (United States)

    Nel, R.; Avison, J.; Harris, P.; Blabla, M.; Hämäläinen, J.

    2017-01-01

    The degrees of equivalence of the AFRIMETS.AUV.A-K5 regional key comparison are reported here as the final report. The scope of the comparison covered the complex pressure sensitivities of two LS1P microphones over the frequency range 2 Hz to 10 kHz in accordance with IEC 61094-2: 2009. Four national metrology institutes from two different regional metrology organisations participated in the comparison. Two LS1P microphones were circulated simultaneously to all the participants in a circular configuration. One of the microphones sensitivity shifted and all results associated with this microphone were subsequently excluded from further analysis and linking. The AFRIMETS.AUV.A-K5 comparison results were linked to the CCAUV.A-K5 comparison results via dual participation in the CCAUV.A-K5 and AFRIMETS.AUV.A-K5 comparisons. The degrees of equivalence, linked to the CCAUV.A-K5 comparison, were calculated for all participants of this comparison. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCAUV, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  12. Fabrication of sub-10 nm metal nanowire arrays with sub-1 nm critical dimension control

    Science.gov (United States)

    Pi, Shuang; Lin, Peng; Xia, Qiangfei

    2016-11-01

    Sub-10 nm metal nanowire arrays are important electrodes for building high density emerging ‘beyond CMOS’ devices. We made Pt nanowire arrays with sub-10 nm feature size using nanoimprint lithography on silicon substrates with 100 nm thick thermal oxide. We further studied the critical dimension (CD) evolution in the fabrication procedure and achieved 0.4 nm CD control, providing a viable solution to the imprint lithography CD challenge as specified by the international technology roadmap for semiconductors. Finally, we fabricated Pt/TiO2/Pt memristor crossbar arrays with the 8 nm electrodes, demonstrating great potential in dimension scaling of this emerging device.

  13. Comparison of Nd:YAG Ceramic Laser Pumped at 885 nm and 808 nm

    Institute of Scientific and Technical Information of China (English)

    ZONG Nan; ZHANG Xiao-Fu; MA Qing-Lei; WANG Bao-Shan; CUI Da-Fu; PENG Qin-Jun; XU Zu-Yan; PAN Yu-Bai; FENG Xi-Qi

    2009-01-01

    Laser performance of 1064 nm domestic Nd: YA G ceramic lasers for 885 nm direct pumping and 808 nm traditional pumping are compared. Higher slope efficiency of 34% and maximum output power of 16.5 W are obtained for the 885nm pump with a 6ram length 1 at% Nd:YAG ceramic. The advantages for 885nm direct pumping are discussed in detail. This pumping scheme for highly doping a Nd:YAG ceramic laser is considered as an available way to generate high power and good beam quality simultaneously.

  14. The CO2 Abundance in Comets C/2012 K1 (PanSTARRS), C/2012 K5 (LINEAR), and 290P/Jager as Measured with Spitzer

    CERN Document Server

    McKay, Adam J; Cochran, Anita L; Bodewits, Dennis; DiSanti, Michael A; Russo, Neil Dello; Lisse, Carey M

    2015-01-01

    We present analysis of observations of CO2 and OI emission in three comets to measure the CO2 abundance and evaluate the possibility of employing observations of OI emission in comets as a proxy for CO2. We obtained NIR imaging sensitive to CO2 of comets C/2012 K1 (PanSTARRS), C/2012 K5 (LINEAR), and 290P/Jager with the IRAC instrument on Spitzer. We acquired observations of OI emission in these comets with the ARCES echelle spectrometer mounted on the 3.5-meter telescope at Apache Point Observatory and observations of OH with the Swift observatory (PanSTARRS) and with Keck HIRES (Jager). The CO2/H2O ratios derived from the Spitzer images are 12.6 +/- 1.3% (PanSTARRS), 28.9 +/- 3.6% (LINEAR), and 31.3 +/- 4.2% (Jager). These abundances are derived under the assumption that contamination from CO emission is negligible. The CO2 abundance for PanSTARRS is close to the average abundance measured in comets at similar heliocentric distance to date, while the abundances measured for LINEAR and Jager are significantl...

  15. Final report on key comparison CCAUV.A-K5: pressure calibration of laboratory standard microphones in the frequency range 2 Hz to 10 kHz

    Science.gov (United States)

    Avison, Janine; Barham, Richard

    2014-01-01

    This document and the accompanying spreadsheets constitute the final report for key comparison CCAUV.A-K5 on the pressure calibration of laboratory standard microphones in the frequency range from 2 Hz to 10 kHz. Twelve national measurement institutes took part in the key comparison and the National Physical Laboratory piloted the project. Two laboratory standard microphones IEC type LS1P were circulated to the participants and results in the form of regular calibration certificates were collected throughout the project. One of the microphones was subsequently deemed to have compromised stability for the purpose of deriving a reference value. Consequently the key comparison reference value (KCRV) has been made based on the weighted mean results for sensitivity level and for sensitivity phase from just one of the microphones. Corresponding degrees of equivalence (DoEs) have also been calculated and are presented. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCAUV, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  16. The radii of the nearby K5V and K7V stars 61 Cyg A & B - CHARA/FLUOR interferometry and CESAM2k modeling

    CERN Document Server

    Kervella, Pierre; Pichon, Bernard; Thévenin, Frédéric; Heiter, Ulrike; Bigot, Lionel; Brummelaar, Theo A Ten; Mcalister, Harold A; Ridgway, Stephen T; Turner, Nils; Sturmann, Judit; Sturmann, Laszlo; Goldfinger, P J; Farrington, Christopher

    2008-01-01

    Context: The main sequence binary star 61 Cyg (K5V+K7V) is our nearest stellar neighbour in the northern hemisphere. This proximity makes it a particularly well suited system for very high accuracy interferometric radius measurements. Aims: Our goal is to constrain the poorly known evolutionary status and age of this bright binary star. Methods: We obtained high accuracy interferometric observations in the infrared K' band, using the CHARA/FLUOR instrument. We then computed evolutionary models of 61 Cyg A & B with the CESAM2k code. As model constraints, we used a combination of observational parameters from classical observation methods (photometry, spectroscopy) as well as our new interferometric radii. Results: The measured limb darkened disk angular diameters are theta_LD(A) = 1.775 +/- 0.013 mas and theta_LD(B) = 1.581 +/- 0.022 mas, respectively for 61 Cyg A and B. Considering the high accuracy parallaxes available, these values translate into photospheric radii of R(A) = 0.665 +/- 0.005 Rsun and R(B...

  17. Characterisation of the effects of ATPA, a GLU(K5) receptor selective agonist, on excitatory synaptic transmission in area CA1 of rat hippocampal slices.

    Science.gov (United States)

    Clarke, V R J; Collingridge, G L

    2002-06-01

    Kainate receptors are involved in a variety of synaptic functions in the CNS including the regulation of excitatory synaptic transmission. Previously we described the depressant action of the GLU(K5) selective agonist (RS)-2-amino-3-(3-hydroxy-5-tert-butylisoxazol-4-yl)propanoic acid (ATPA) on synaptic transmission in the Schaffer collateral-commissural pathway of rat hippocampal slices. In the present study we report several new features of the actions of ATPA at this synapse. Firstly, the effectiveness of ATPA is developmentally regulated. Secondly, the effects of ATPA decline during prolonged or repeated applications. Thirdly, the effects of ATPA are not mediated indirectly via activation of GABA(A), GABA(B), muscarinic or adenosine A(1) receptors. Fourthly, elevating extracellular Ca(2+) from 2 to 4 mM antagonises the effects of ATPA. Some differences between the actions of ATPA and kainate on synaptic transmission in the Schaffer collateral-commissural pathway are also noted.

  18. Optical lithography at a 126-nm wavelength

    Science.gov (United States)

    Kang, Hoyoung; Bourov, Anatoly; Smith, Bruce W.

    2001-08-01

    There is a window of opportunity for optical lithography between wavelengths of 100 nm and 157 nm that warrants exploration as a next generation technology. We will present activities underway to explore the feasibility of VUV optical lithography in this region with respect to source, optical design, materials, processes, masks, resolution enhancement, and compatibility with existing technologies. We have constructed a small field prototype lithography system using the second continuum 126nm emission wavelength of the Argon excimer. This has been accomplished using a small dielectric barrier discharge lamp with output on the order of 10mW/cm2 and small field catoptric imaging systems based on a modified Cassegrain system. Capacitance focus gauge and piezo electric stage has been installed for fine focusing. In order to achieve sub-half wavelength resolution that would be required to compete with 157nm lithography and others, we have started exploring the feasibility of using liquefied noble gas immersion fluids to increase effective value of lens numerical aperture by factors approaching 1.4x. Conventional silylation process works well with 126nm with high sensitivity. Chemically amplified DUV negative resist looks very good material for 126 nm. Initial contact printing image shows good selectivity and process control. An effort is also underway to explore the use of inorganic resist materials, as silver halide material for instance, to replace the conventional polymeric imaging systems that are currently employed at longer wavelengths, but may be problematic at these VUV wavelengths. Early accomplishments are encouraging. Prototype optical research tools can be used to reveal issues involved with 126nm lithography and solve initial problems. Though many challenges do exist at this short wavelength, it is quite feasible that lithography at this wavelength could meet the part of the needs of future device generations.

  19. H3K4 dimethylation in hepatocellular carcinoma is rare compared with other hepatobiliary and gastrointestinal carcinomas and correlates with expression of the methylase Ash2 and the demethylase LSD1.

    Science.gov (United States)

    Magerl, Christian; Ellinger, Jörg; Braunschweig, Till; Kremmer, Elisabeth; Koch, Lin Kristin; Höller, Tobias; Büttner, Reinhard; Lüscher, Bernhard; Gütgemann, Ines

    2010-02-01

    Methylation of core histones regulates chromatin structure and gene expression. Recent studies have demonstrated that these methylation patterns have prognostic value for some tumors. Therefore, we investigated dimethylation of histone H3 at lysine 4 (H3K4diMe) and H3K4 methylating (Ash2 complex) and demethylating enzymes (LSD1) in carcinomas of the hepatic and gastrointestinal tract. High levels of H3K4diMe were rarely observed in 15.7% of hepatocellular carcinoma (8/51) unlike other carcinomas including, in ascending order, cholangiocellular carcinoma/adenocarcinoma of the extrahepatic biliary tract, gastric carcinoma, pancreatic ductal adenocarcinoma, and neuroendocrine carcinoma (P carcinomas (38/45) and correlated directly with H3K4diMe modification (correlation coefficient r = 0.53) and LSD1 expression (r = 0.35). In contrast to other carcinomas, 65.9% (29/44) of hepatocellular carcinomas analyzed showed no LSD1 expression (P carcinomas without LSD1 expression appeared to be frequently Ash2 and H3K4diMe weak or negative (P = .004). In summary, high H3K4diMe expression is rare in hepatocellular carcinoma compared with other carcinomas (negative predictive value 92.3%), which may aid in the differential diagnosis. Lack of H3K4diMe is possibly due to complex epigenetic regulation involving Ash2 and LSD1.

  20. Final Report on the Key Comparison CCM.P-K4.2012 in Absolute Pressure from 1 Pa to 10 kPa.

    Science.gov (United States)

    Ricker, Jacob; Hendricks, Jay; Bock, Thomas; Dominik, Pražák; Kobata, Tokihiko; Torres, Jorge; Sadkovskaya, Irina

    2017-01-01

    The report summarizes the Consultative Committee for Mass (CCM) key comparison CCM.P-K4.2012 for absolute pressure spanning the range of 1 Pa to 10 000 Pa. The comparison was carried out at six National Metrology Institutes (NMIs), including National Institute of Standards and Technology (NIST), Physikalisch-Technische Bundesanstalt (PTB), Czech Metrology Institute (CMI), National Metrology Institute of Japan (NMIJ), Centro Nacional de Metrología (CENAM), and DI Mendeleyev Institute for Metrology (VNIIM). The comparison was made via a calibrated transfer standard measured at each of the NMIs facilities using their laboratory standard during the period May 2012 to September 2013. The transfer package constructed for this comparison preformed as designed and provided a stable artifact to compare laboratory standards. Overall the participants were found to be statistically equivalent to the key comparison reference value.

  1. Radiation Failures in Intel 14nm Microprocessors

    Science.gov (United States)

    Bossev, Dobrin P.; Duncan, Adam R.; Gadlage, Matthew J.; Roach, Austin H.; Kay, Matthew J.; Szabo, Carl; Berger, Tammy J.; York, Darin A.; Williams, Aaron; LaBel, K.; Ingalls, James D.

    2016-01-01

    In this study the 14 nm Intel Broadwell 5th generation core series 5005U-i3 and 5200U-i5 was mounted on Dell Inspiron laptops, MSI Cubi and Gigabyte Brix barebones and tested with Windows 8 and CentOS7 at idle. Heavy-ion-induced hard- and catastrophic failures do not appear to be related to the Intel 14nm Tri-Gate FinFET process. They originate from a small (9 m 140 m) area on the 32nm planar PCH die (not the CPU) as initially speculated. The hard failures seem to be due to a SEE but the exact physical mechanism has yet to be identified. Some possibilities include latch-ups, charge ion trapping or implantation, ion channels, or a combination of those (in biased conditions). The mechanism of the catastrophic failures seems related to the presence of electric power (1.05V core voltage). The 1064 nm laser mimics ionization radiation and induces soft- and hard failures as a direct result of electron-hole pair production, not heat. The 14nm FinFET processes continue to look promising for space radiation environments.

  2. Chromosomal distribution of H3K4me2, H3K9me2 and 5-methylcytosine: variations associated with polyploidy and hybridization in Brachiaria (Poaceae).

    Science.gov (United States)

    de Paula, Cristina Maria Pinto; Souza Sobrinho, Fausto; Techio, Vânia Helena

    2016-06-01

    Assessment of chromosomal distribution of modified histones and 5-methylcytosine shown that there are diversification of chromosomal types among species of Brachiaria and its interspecific hybrids. Histone post-translational modifications and DNA methylation are epigenetic processes that are involved in structural and functional organization of the genome. This study compared the chromosomal distribution of modified histones and 5-methylcytosine (5-mCyt) in species and interspecific hybrids of Brachiaria with different ploidy levels and reproduction modes. The relation between H3K9me2 and 5-mCyt was observed in the nucleolus organizer region, centromeric central domain and pericentromeric region. H3K4me2 was detected in euchromatic domains, mainly in the terminal chromosomal regions. Comparison of chromosomal distribution among species and hybrids showed greater variation of chromosomal types for the H3K9me2 in B. decumbens (tetraploid and apomictic species) and the 963 hybrid, while, for the H3K4me2, the variation was higher in B. brizantha and B. decumbens (tetraploid and apomictic species) and 963 hybrid. The chromosome distribution of 5-mCyt was similar between B. brizantha and B. decumbens, which differ from the distribution observed in B. ruziziensis (diploid and sexual species). Significant alterations in DNA methylation were observed in the artificially tetraploidized B. ruziziensis and in the interspecific hybrids, possibly as result of hybridization and polyploidization processes. The monitoring of histone modifications and DNA methylation allowed categorizing nuclear and chromosomal distribution of these epigenetic marks, thus contributing to the knowledge of composition and structure of the genome/epigenome of Brachiaria species and hybrids. These data can be useful for speciation and genome evolution studies in genus Brachiaria, and represent important markers to explore relationships between genomes.

  3. Diode laser (980nm) cartilage reshaping

    Science.gov (United States)

    El Kharbotly, A.; El Tayeb, T.; Mostafa, Y.; Hesham, I.

    2011-03-01

    Loss of facial or ear cartilage due to trauma or surgery is a major challenge to the otolaryngologists and plastic surgeons as the complicated geometric contours are difficult to be animated. Diode laser (980 nm) has been proven effective in reshaping and maintaining the new geometric shape achieved by laser. This study focused on determining the optimum laser parameters needed for cartilage reshaping with a controlled water cooling system. Harvested animal cartilages were angulated with different degrees and irradiated with different diode laser powers (980nm, 4x8mm spot size). The cartilage specimens were maintained in a deformation angle for two hours after irradiation then released for another two hours. They were serially measured and photographed. High-power Diode laser irradiation with water cooling is a cheep and effective method for reshaping the cartilage needed for reconstruction of difficult situations in otorhinolaryngologic surgery. Key words: cartilage,diode laser (980nm), reshaping.

  4. Cell surface display of cold-active esterase EstPc with the use of a new autotransporter from Psychrobacter cryohalolentis K5(T).

    Science.gov (United States)

    Petrovskaya, L E; Novototskaya-Vlasova, K A; Kryukova, E A; Rivkina, E M; Dolgikh, D A; Kirpichnikov, M P

    2015-01-01

    We have cloned the gene coding for AT877-a new predicted member of the autotransporter protein family with an esterase passenger domain from permafrost bacterium Psychrobacter cryohalolentis K5(T). Expression of AT877 gene in Escherichia coli resulted in accumulation of the recombinant autotransporter in the outer membrane fraction and at the surface of the induced cells. AT877 displayed maximum hydrolytic activity toward medium-chain p-nitrophenyl esters (C8-C10) at 50 °C and was resistant to the presence of several metal ions, organic solvents and detergents. Previously, we have described a cold-active esterase EstPc from the same bacterium which possesses high activity at low temperatures and relatively high thermal stability. To construct a cell surface display system for EstPc, the hybrid autotransporter gene coding for EstPc with the α-helical linker and the translocator domain from AT877 was constructed and expressed in E. coli. According to the results of the cell fractionation studies and esterase activity measurements, the EstPc passenger was successfully displayed at the surface of the induced cells. It demonstrated a temperature optimum at 15-25 °C and a substrate preference toward p-nitrophenyl butyrate (C4). Obtained results provide a new example of the biotechnologically relevant enzyme from the permafrost microbial community with potential applications for the conversion of short- and medium-chain ester substrates and a basis for the construction of a new cell surface display platform.

  5. Liquid Carbon Reflectivity at 19 nm

    Directory of Open Access Journals (Sweden)

    Riccardo Mincigrucci

    2015-01-01

    Full Text Available We hereby report on a pump-probe reflectivity experiment conducted on amorphous carbon, using a 780 nm laser as a pump and a 19 nm FEL emission as probe. Measurements were performed at 50 degrees with respect to the surface normal to have an un-pumped reflectivity higher than 0.5%. A sub-10 fs time synchronization error could be obtained exploiting the nearly jitter-free capabilities of FERMI. EUV FEL-based experiments open the way to study the behaviour of a liquid carbon phase being unaffected by plasma screening.

  6. 1550-nm wavelength-tunable HCG VCSELs

    Science.gov (United States)

    Chase, Christopher; Rao, Yi; Huang, Michael; Chang-Hasnain, Connie

    2014-02-01

    We demonstrate wavelength-tunable VCSELs using high contrast gratings (HCGs) as the top output mirror on VCSELs, operating at 1550 nm. Tunable HCG VCSELs with a ~25 nm mechanical tuning range as well as VCSELs with 2 mW output power were realized. Error-free operation of an optical link using directly-modulated tunable HCG VCSELs transmitting at 1.25 Gbps over 18 channels spaced by 100 GHz and transmitted over 20 km of single mode fiber is demonstrated, showing the suitability of the HCG tunable VCSEL as a low cost source for WDM communications systems.

  7. Design of an 1800nm Raman amplifier

    DEFF Research Database (Denmark)

    Svane, Ask Sebastian; Rottwitt, Karsten

    2013-01-01

    We present the experimental results for a Raman amplifier that operates at 1810 nm and is pumped by a Raman fiber laser at 1680 nm. Both the pump laser and the Raman amplifier is polarization maintaining. A challenge when scaling Raman amplifiers to longer wavelengths is the increase...... in transmission loss, but also the reduction in the Raman gain coefficient as the amplifier wavelength is increased. Both polarization components of the Raman gain is characterized, initially for linearly co-polarized signal and pump, subsequently linearly polarized orthogonal signal and pump. The noise...

  8. External cavity diode laser around 657 nm

    Institute of Scientific and Technical Information of China (English)

    Desheng Lǖ (吕德胜); Kaikai Huang (黄凯凯); Fengzhi Wang (王凤芝); DonghaiYang (杨东海)

    2003-01-01

    Operating a laser diode in an external cavity, which provides frequency-selective feedback, is a very effective method to tune the laser frequency to a range far from its free running frequency. For the Ca atomic Ramsey spectroscopy experiment, we have constructed a 657-nm laser system based on the LittmanMetcalf configuration with a 660-nm commercial laser diode. Continuously 10-GHz tuning range was achieved with about 100-kHz spectral linewidth, measured with beat-note spectrum of two identical laser systems.

  9. Effect of sleep deprivation on H3K9 and H3K4 methylation in the different brain region of rats%REM期睡眠剥夺对大鼠不同脑区组蛋白H3K9和H3K4甲基化水平的影响

    Institute of Scientific and Technical Information of China (English)

    马安东; 马妮; 史明; 田丰仓; 吴中亮

    2016-01-01

    目的:研究REM期睡眠剥夺对成年大鼠不同脑区包括海马、腹内侧前额叶皮质、下丘脑和中缝核群的组蛋白H3K9和H3K4三甲基化水平的影响.方法:将成年SD大鼠随机分为正常对照组(con)、睡眠剥夺1d组(SD1 d)、睡眠剥夺3d组(SD3 d)、睡眠剥夺6d组(SD6 d).采用改良式多平台水环境法建立REM期睡眠剥夺模型,分别于睡眠剥夺后1、3、6d断头取脑,用免疫荧光染色与Western Blot检测大鼠海马、腹内侧前额叶皮质、下丘脑和中缝核群H3K9和H3K4三甲基化水平的变化,最终结果进行统计学分析.结果:Western Blot结果显示:(1)海马、腹内侧前额叶皮质三个SD组H3K9三甲基化水平均低于control组(P<0.05),H3K4三甲基化水平均高于control组(P<0.05);(2)下丘脑三个SD组H3K9三甲基化水平均低于control组(P<0.01),但SD1 d组H3K4三甲基化水平高于control组(P<0.05),SD3 d、SD6 d组H3K4三甲基化水平低于control组(P<0.05);(3)中缝核群三个SD组H3K9和H3K4三甲基化水平均高于control组(P<0.05).对海马与下丘脑免疫荧光验证H3K9三甲基化结果与Western Blot结果趋势一致,且说明细胞无减少.结论:睡眠剥夺可能与海马、腹内侧前额叶皮质、下丘脑和中缝核群的组蛋白H3K9和H3K4三甲基化水平密切相关.

  10. MEPHISTO spectromicroscope reaches 20 nm lateral resolution

    Science.gov (United States)

    De Stasio, Gelsomina; Perfetti, Luca; Gilbert, B.; Fauchoux, O.; Capozi, M.; Perfetti, P.; Margaritondo, G.; Tonner, B. P.

    1999-03-01

    The recently described tests of the synchrotron imaging photoelectron spectromicroscope MEPHISTO (Microscope à Emission de PHotoélectrons par Illumination Synchrotronique de Type Onduleur) were complemented by further resolution improvements and tests, which brought the lateral resolution down to 20 nm. Images and line plot profiles demonstrate such performance.

  11. Dot1-dependent histone H3K79 methylation promotes the formation of meiotic double-strand breaks in the absence of histone H3K4 methylation in budding yeast.

    Directory of Open Access Journals (Sweden)

    Mohammad Bani Ismail

    Full Text Available Epigenetic marks such as histone modifications play roles in various chromosome dynamics in mitosis and meiosis. Methylation of histones H3 at positions K4 and K79 is involved in the initiation of recombination and the recombination checkpoint, respectively, during meiosis in the budding yeast. Set1 promotes H3K4 methylation while Dot1 promotes H3K79 methylation. In this study, we carried out detailed analyses of meiosis in mutants of the SET1 and DOT1 genes as well as methylation-defective mutants of histone H3. We confirmed the role of Set1-dependent H3K4 methylation in the formation of double-strand breaks (DSBs in meiosis for the initiation of meiotic recombination, and we showed the involvement of Dot1 (H3K79 methylation in DSB formation in the absence of Set1-dependent H3K4 methylation. In addition, we showed that the histone H3K4 methylation-defective mutants are defective in SC elongation, although they seem to have moderate reduction of DSBs. This suggests that high levels of DSBs mediated by histone H3K4 methylation promote SC elongation.

  12. Photoresist outgassing at 157 nm exposure

    Science.gov (United States)

    Hien, Stefan; Angood, Steve; Ashworth, Dominic; Basset, Steve; Bloomstein, Theodore M.; Dean, Kim R.; Kunz, Roderick R.; Miller, Daniel A.; Patel, Shashikant; Rich, Georgia K.

    2001-08-01

    Contamination of optical elements during photoresist exposure is a serious issue in optical lithography. The outgassing of photoresist has been identified as a problem at 248nm and 193nm in production because the organic films that can be formed on an exposure lens can cause transmission loss and sever image distortion. At these exposure energies, the excitation of the photo acid generator, formation of acid, and cleavage of the protecting group are highly selective processes. At 157nm, the exposure energy is much higher (7.9 eV compared to 6.4 eV at 193nm) and it is known from laser ablation experiments that direct laser cleavage of sigma bonds occurs. The fragments formed during this irradiation can be considered as effective laser deposition precursors even in the mid ppb level. In this study, methods to quantify photoresist outgassing at 157 nm are discussed. Three criteria have been set up at International SEMATECH to protect lens contamination and to determine the severity of photoresist outgassing. First, we measured film thickness loss as a function of exposure dose for a variety of materials. In a second test we studied the molecular composition of the outgassing fragments with an exposure chamber coupled to a gas chromatograph and a mass spectrometer detector. Our third method was a deposition test of outgassing vapors on a CaF2 proof plate followed by analysis using VUV and X-ray photoelectron spectroscopies (XPS). With this technique we found deposits for many different resists. Our main focus is on F- and Si- containing resists. Both material classes form deposits especially if these atoms are bound to the polymer side chains. Whereas the F-containing films can be cleaned off under 157nm irradiation, cleaning of Si-containing films mainly produces SiO2. Our cleaning studies of plasma deposited F-containing organic films on SiO2 did not indicate damage of this surface by the possible formation of HF. Despite that we strongly recommend engineering

  13. Photochemistry of acrylates at 222 nm

    Science.gov (United States)

    Knolle, Wolfgang; Naumov, Sergej; Madani, Mohamed; von Sonntag, Clemens

    2005-07-01

    Excimer lamps as monochromatic UV sources with an intense short-wavelength emission (especially KrCl∗, 222 nm) allow a photoinitiator-free initiation of the acrylate polymerisation. Laser photolysis (KrCl∗ excimer laser, pulse width 20 ns, up to 5 mJ per pulse) gives rise to similar transient spectra (λmax ≈ 280 nm) for all acrylates studied. As the rather unspecific spectra do not allow conclusions as to the main reaction channel, a product study has been performed by GC-MS following steady-state photolysis of acrylate solutions in acetonitrile, methanol and n-hexane. Somewhat unexpected, α-cleavage seems to be a main reaction channel, and quantum chemical calculations show that such a reaction can occur from either the excited singlet state or the unrelaxed triplet state, but not from the relaxed triplet state that is observed spectroscopically. A reaction scheme accounting for the observed products is presented.

  14. Actively Pumped Optical Filters at 532 nm

    Science.gov (United States)

    Billmers, Richard I.; Gayen, S. K.; Contarino, Vincent M.; Scharpf, William J.; Squicciarini, Martin F.; Allocca, David A.

    1995-01-01

    The operation of two narrow-band optical filters at 532.33 nm is presented. Both of these filters operate on the 4P(sub 1/2) to 8S(sub 1/2) excited-state transition in potassium vapor. One of the filters is based on excited-state Faraday effect, and requires the application of an external axial magnetic field. The peak transmission of this filter is approximately 3.5% with a linewidth of less than 10 GHz. The second filter does not require a magnetic field for its operation, but readily attains peak transmissions of 25-30%. The 4P(sub 1/2) state is excited by a 769.9 nm light pulse which is linearly polarized for the first scheme and circularly polarized for the second.

  15. Final report on the key comparison CCM.P-K4.2012 in absolute pressure from 1 Pa to 10 kPa

    Science.gov (United States)

    Ricker, Jacob; Hendricks, Jay; Bock, Thomas; Dominik, Pražák; Kobata, Tokihiko; Torres, Jorge; Sadkovskaya, Irina

    2017-01-01

    The report summarizes the Consultative Committee for Mass (CCM) key comparison CCM.P-K4.2012 for absolute pressure spanning the range of 1 Pa to 10 000 Pa. The comparison was carried out at six National Metrology Institutes (NMIs), including National Institute of Standards and Technology (NIST), Physikalisch-Technische Bundesanstalt (PTB), Czech Metrology Institute (CMI), National Metrology Institute of Japan (NMIJ), Centro Nacional de Metrología (CENAM), and DI Mendeleyev Institute for Metrology (VNIIM). The comparison was made via a calibrated transfer standard measured at each of the NMIs facilities using their laboratory standard during the period May 2012 to September 2013. The transfer package constructed for this comparison preformed as designed and provided a stable artifact to compare laboratory standards. Overall the participants were found to be statistically equivalent to the key comparison reference value. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  16. Magnetic transition in K4Cu4OCl10: A model system of three-dimensional spin-(1)/(2) tetrahedra

    Science.gov (United States)

    Fujihala, Masayoshi; Zheng, Xu-Guang; Morodomi, Hiroki; Kawae, Tatsuya; Watanabe, Isao

    2013-04-01

    Isolated spin tetrahedral systems with weak intertetrahedral couplings, as have been reported for Cu2Te2O5X2 (X = Cl, Br) and the related compound Cu4Te5O12Cl4, have received much attention recently because they represent an interesting class of magnets that consist of weakly coupled magnetic clusters and, in particular, they can directly demonstrate the interplay of intertetrahedral couplings with built-in tetrahedral frustration. However, there is much debate about the structural low dimensionality of the Cu-Te-O-Cl(Br) compounds and its effect on the magnetism of the material. Here, we present a model spin tetrahedral system K4Cu4OCl10, with almost isotropic magnetic coupling within the tetrahedron and three-dimensional connection of the tetrahedra. The system enters a spin-singlet state with a susceptibility maximum at Tmax = 11 K, and then enters an antiferromagnetic order at TN = 4.4 K. The ratio TN/Tmax = 0.40 is close to the TN/Tmax = 0.38 for Cu2Te2O5Br2, which is viewed as an indicator of closeness to quantum criticality. Evidence in muon-spin rotation or relaxation suggests an incommensurate ordering. This work shows that the previously revealed ground state in anisotropically structured Cu2Te2O5X2 compounds also exists in an isotropic spin tetrahedral system.

  17. Radiation Status of Sub-65 nm Electronics

    Science.gov (United States)

    Pellish, Jonathan A.

    2011-01-01

    Ultra-scaled complementary metal oxide semiconductor (CMOS) includes commercial foundry capabilities at and below the 65 nm technology node Radiation evaluations take place using standard products and test characterization vehicles (memories, logic/latch chains, etc.) NEPP focus is two-fold: (1) Conduct early radiation evaluations to ascertain viability for future NASA missions (i.e. leverage commercial technology development). (2) Uncover gaps in current testing methodologies and mechanism comprehension -- early risk mitigation.

  18. Weak-signal conversion from 1550nm to 532nm with 84% efficiency

    CERN Document Server

    Samblowski, Aiko; Baune, Christoph; Fiurasek, Jaromir; Schnabel, Roman

    2013-01-01

    We report on the experimental frequency conversion of a dim, coherent continuous-wave light field from 1550nm to 532nm with an external photon-number conversion efficiency of (84.4 +/- 1.5)%. We used sum-frequency generation, which was realized in a standing-wave cavity built around a periodically poled type I potassium titanyl phosphate (PPKTP) crystal, pumped by an intense field at 810 nm. Our result is in full agreement with a numerical model. For optimized cavity coupler reflectivities it predicts a conversion efficiency of up to 93% using the same PPKTP crystal.

  19. Comparative Study of CMOS Op-Amp In 45nm And 180 Nm Technology

    Directory of Open Access Journals (Sweden)

    Siddharth

    2014-07-01

    Full Text Available In this paper we have provided a method for designing a Two Stage CMOS Operational Amplifier which operates at 1.8V power supply using Cadence Virtuoso 45nm CMOS technology. Further, designing the two stage op-amp for the same power supply using Cadence Virtuoso 180nm CMOS Technology, keeping the slew rate of the op-amp same as that 45nm technology. The trade-off curves are computed between various characteristics such as Gain, Phase Margin,GBW,3db Gain etc. and the results obtained for 45n CMOS Technology is compared with those obtained for 180nm CMOS Technology It has been demonstrated that on lowering the technology and keeping the slew rate constant, the Power dissipation decreases.

  20. STEM视角下的美国科学课程教材分析--以FOSS K-5年级科学教材为例%An Analysis Study of American Science Textbooks from STEM Perspective-Taking the FOSS K-5 Edition as an Example

    Institute of Scientific and Technical Information of China (English)

    周鹏琴; 徐唱; 张韵; 李芒

    2016-01-01

    为探究美国科学课程的教学内容特点,从而了解美国科学教育的发展状况,该文以STEM教育为视角,应用定量与质性相结合的研究方法,对美国FOSS K-5年级加利福尼亚2007年版的科学教材SCIENCE RESOURCES知识点和教学活动等内容进行分析。从教材知识点的整体分布,物质科学、生命科学、地球科学三大主题知识点分布以及教学活动等其他内容三个方面获得研究结果。结合已有研究,得出结论:教材注重学科间的相互渗透与干涉,强调STEM教育视野下综合性的科学素养,体现STEM教育对生活的价值。最后获得启示:我国科学教育发展应树立正确的科学教育理念、提高学科地位、建立完备的课程体系、充分发挥教师的作用、实现从知识到实践的跨越、加强实证研究。%In order to explore the teaching content characteristics of American Science Curriculum and understand the development of Science Education in the United States, this study analyzed the knowledge points and teaching activities of FOSS Grades K-5 California Edition © 2007 “SCIENCE RESOURCES” from STEM perspective, combining quantitative and qualitative research methods. The knowledge points of the three main themes (Physical Sciences, Life Sciences and Earth Sciences) and teaching activities in the textbooks were coded and counted. The result shows that: American science textbook focus on the mutual penetration and interference between subjects, the comprehensive scientiifc literacy, and the value of STEM education to life. Finally, attain the enlightenment about developing the Science Education: we should establish correct science educational concept, improve the subject status, establish a comprehensive curriculum system, give full play to the role of teachers, realize the leap from knowledge to practice and reinforce the empirical research.

  1. Optical issues of thin organic pellicles in 45-nm and 32-nm immersion lithography

    Science.gov (United States)

    Lucas, Kevin; Gordon, Joseph S.; Conley, Will; Saied, Mazen; Warrick, Scott; Pochkowski, Mike; Smith, Mark D.; West, Craig; Kalk, Franklin; Kuijten, Jan Pieter

    2006-10-01

    The semiconductor industry will soon be putting >=1.07NA 193nm immersion lithography systems into production for the 45nm device node and in about three years will be putting >=1.30NA systems into production for the 32nm device node. For these very high NA systems, the maximum angle of light incident on a 4X reticle will reach ~16 degrees and ~20 degrees for the 45nm and 32nm nodes respectively. These angles can no longer be accurately approximated by an assumption of normal incidence. The optical diffraction and thin film effects of high incident angles on the wafer and on the photomask have been studied by many different authors. Extensive previous work has also investigated the impact of high angles upon hard (e.g., F-doped silica) thick (>700μm) pellicles for 157nm lithography, e.g.,. However, the interaction of these high incident angles with traditional thin (< 1μm) organic pellicles has not been widely discussed in the literature. In this paper we analyze the impact of traditional thin organic pellicles in the imaging plane for hyper-NA immersion lithography at the 45nm and 32nm nodes. The use of existing pellicles with hyper-NA imaging is shown to have a definite negative impact upon lithographic CD control and optical proximity correction (OPC) model accuracy. This is due to the traditional method of setting organic pellicle thickness to optimize normally incident light transmission intensity. Due to thin film interference effects with hyper-NA angles, this traditional pellicle optimization method will induce a loss of high spatial frequency (i.e., high transmitted angle) intensity which is similar in negative impact to a strong lens apodization effect. Therefore, using simulation we investigate different pellicle manufacturing options (e.g., multi-layer pellicle films) and OPC modeling options to reduce the high spatial frequency loss and its impact.

  2. Final report on the EURAMET.M.FF-K4.2.2014 volume comparison at 100 μL—calibration of micropipettes

    Science.gov (United States)

    Batista, Elsa; Matus, Michael; Metaxiotou, Zoe; Tudor, Maria; Lenard, Elzbieta; Buker, Oliver; Wennergren, Per; Piluri, Erinda; Miteva, Mariana; Vicarova, Martina; Vospĕlová, Alena; Turnsek, Urska; Micic, Ljiljana; Grue, Lise-Lote; Mihailovic, Mirjana; Sarevska, Anastazija

    2017-01-01

    During the EURAMET TC-F meeting of 2014 and following the finalization of CCM.FF-K4.2.2011 comparison, it was agreed to start a Regional Key Comparison (KC) on volume measurements using two 100 μL micropipettes (piston pipettes) allowing the participating laboratories to assess the agreement of their results and uncertainties. Two 100 μL micropipettes were tested by 15 participants. One participant was not a member or associate member of the BIPM and was be removed from this report. The comparison started in July 2015 and ended in March 2016. The Volume and Flow Laboratory of the Portuguese Institute for Quality (IPQ) was the pilot laboratory and performed the initial and final measurements of the micropipettes. The micropipettes showed a stable volume during the whole comparison, which was confirmed by the results from the pilot laboratory. The original results of all participant NMIs were corrected to the standard atmospheric pressure in order to compare results under the same calibration conditions, and the contribution of the 'process-related handling contribution' was added to the uncertainty budget of each participant. In general the declared CMCs are in accordance with the KCDB. For the micropipette 354828Z, two laboratories had inconsistent results. For micropipette 354853Z, three laboratories had inconsistent results. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  3. RSV-Induced H3K4 Demethylase KDM5B Leads to Regulation of Dendritic Cell-Derived Innate Cytokines and Exacerbates Pathogenesis In Vivo.

    Directory of Open Access Journals (Sweden)

    Catherine Ptaschinski

    2015-06-01

    Full Text Available Respiratory syncytial virus (RSV infection can result in severe disease partially due to its ability to interfere with the initiation of Th1 responses targeting the production of type I interferons (IFN and promoting a Th2 immune environment. Epigenetic modulation of gene transcription has been shown to be important in regulating inflammatory pathways. RSV-infected bone marrow-derived DCs (BMDCs upregulated expression of Kdm5b/Jarid1b H3K4 demethylase. Kdm5b-specific siRNA inhibition in BMDC led to a 10-fold increase in IFN-β as well as increases in IL-6 and TNF-α compared to control-transfected cells. The generation of Kdm5bfl/fl-CD11c-Cre+ mice recapitulated the latter results during in vitro DC activation showing innate cytokine modulation. In vivo, infection of Kdm5bfl/fl-CD11c-Cre+ mice with RSV resulted in higher production of IFN-γ and reduced IL-4 and IL-5 compared to littermate controls, with significantly decreased inflammation, IL-13, and mucus production in the lungs. Sensitization with RSV-infected DCs into the airways of naïve mice led to an exacerbated response when mice were challenged with live RSV infection. When Kdm5b was blocked in DCs with siRNA or DCs from Kdm5bfl/fl-CD11c-CRE mice were used, the exacerbated response was abrogated. Importantly, human monocyte-derived DCs treated with a chemical inhibitor for KDM5B resulted in increased innate cytokine levels as well as elicited decreased Th2 cytokines when co-cultured with RSV reactivated CD4+ T cells. These results suggest that KDM5B acts to repress type I IFN and other innate cytokines to promote an altered immune response following RSV infection that contributes to development of chronic disease.

  4. 248nm silicon photoablation: Microstructuring basics

    Energy Technology Data Exchange (ETDEWEB)

    Poopalan, P.; Najamudin, S. H.; Wahab, Y.; Mazalan, M. [Advanced Multidisciplinary MEMS-Based Integrated Electronic NCER Centre of Excellent (AMBIENCE), School of Microelectronic Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis (Malaysia)

    2015-05-15

    248nm pulses from a KrF excimer laser was used to ablate a Si wafer in order to ascertain the laser pulse and energy effects for use as a microstructuring tool for MEMS fabrication. The laser pulses were varied between two different energy levels of 8mJ and 4mJ while the number of pulses for ablation was varied. The corresponding ablated depths were found to range between 11 µm and 49 µm, depending on the demagnified beam fluence.

  5. Harmonic Inverse FEL Interaction at 800nm

    CERN Document Server

    Sears, C M S; Siemann, R; Spencer, J E

    2005-01-01

    The inverse Free Electron Laser (IFEL) interaction has recently been proposed and demonstrated as a premodulator for High Gain Harmonic Generation (HGHG) experiments. These experiments utilized the fundamental of the interaction between the laser field and electron bunch. In the current experiment, we explore the higher order resonances of the IFEL interaction from a 3 period, 1.8 centimeter wavelength undulator with a picosecond, 0.25 mJ/pulse laser at 800nm. The resonances are observed by adjusting the gap of the undulator while keeping the beam energy constant. The harmonic IFEL can add flexibility to HGHG FEL design.

  6. Advanced processes for 193-nm immersion lithography

    CERN Document Server

    Wei, Yayi

    2009-01-01

    This book is a comprehensive guide to advanced processes and materials used in 193-nm immersion lithography (193i). It is an important text for those new to the field as well as for current practitioners who want to broaden their understanding of this latest technology. The book can be used as course material for graduate students of electrical engineering, material sciences, physics, chemistry, and microelectronics engineering and can also be used to train engineers involved in the manufacture of integrated circuits. It provides techniques for selecting critical materials (topcoats, photoresi

  7. Role of RbBP5 and H3K4me3 in the vicinity of Snail transcription start site during epithelial-mesenchymal transition in prostate cancer cell.

    Science.gov (United States)

    Li, Dong; Sun, Hui; Sun, Wen-Jing; Bao, Hong-Bo; Si, Shu-Han; Fan, Jia-Lin; Lin, Ping; Cui, Rong-Jun; Pan, Yu-Jia; Wen, Si-Min; Zheng, Xiu-Lan; Yu, Xiao-Guang

    2016-10-04

    EMT (epithelial-mesenchymal transition) occurs in a wide range of tumor types, and has been shown to be crucial for metastasis. Epigenetic modifications of histones contribute to chromatin structure and result in the alterations in gene expression. Tri-methylation of histone H3 lysine 4 (H3K4me3) is associated with the promoters of actively transcribed genes and can serve as a transcriptional on/off switch. RbBP5 is a component of the COMPASS/ -like complex, which catalyzes H3K4me3 formation. In this study, we found that in the process of TGF-Beta1 induced EMT in the prostate cancer cell line DU145, H3K4me3 enrichment and RbBP5 binding increased in the vicinity of Snail (SNAI1) transcription start site. Knocking-down of RbBP5 notably decreased Snail expression and EMT. Recruitment of RbBP5 and formation of H3K4me3 at Snail TSS during EMT depend on binding of SMAD2/3 and CBP at Snail TSS. This study links the SMAD2/3 signal with Snail transcription via a histone modification - H3K4me3. Furthermore, our research also demonstrates that RbBP5 and even WRAD may be a promising therapeutic candidates in treating prostate cancer metastasis, and that DU145 cells maintain their incomplete mesenchymal state in an auto/ paracrine manner.

  8. TCSPC FLIM in the wavelength range from 800 nm to 1700 nm (Conference Presentation)

    Science.gov (United States)

    Becker, Wolfgang; Shcheslavsky, Vladislav

    2016-03-01

    Excitation and detection in the wavelength range above 800nm is a convenient and relatively inexpensive way to increase the penetration depth in optical microscopy. Moreover, detection at long wavelength avoids the problem that tissue autofluorescence contaminates the signals from endogenous fluorescence probes. FLIM at NIR wavelength may therefore be complementary to multiphoton microscopy, especially if the lifetimes of NIR fluorophores report biological parameters of the tissue structures they are bound to. Unfortunately, neither the excitation sources nor the detectors of standard confocal and multiphoton laser scanning systems are directly suitable for excitation and detection of NIR fluorescence. Most of these problems can be solved, however, by using ps diode lasers or Ti:Sapphire lasers at their fundamental wavelength, and NIR-sensitive detectors. With NIR-sensitive PMTs the detection wavelength range can be extended up to 900 nm, with InGaAs SPAD detectors up to 1700 nm. Here, we demonstrate the use of a combination of laser scanning, multi-dimensional TCSPC, and advanced excitation sources and detectors for FLIM at up to 1700 nm. The performance was tested at tissue samples incubated with NIR dyes. The fluorescence lifetimes generally get shorter with increasing absorption and emission wavelengths of the dyes. For the cyanine dye IR1061, absorbing around 1060 nm, the lifetime was found to be as short as 70 ps. Nevertheless the fluorescence decay could still be clearly detected. Almost all dyes showed clear lifetime changes depending on the binding to different tissue constituents.

  9. Total spectral radiant flux measurements on Xe excimer lamps from 115 nm to 1000 nm

    Science.gov (United States)

    Trampert, Klaus E.; Paravia, Mark; Daub, Rüdiger; Heering, Wolfgang

    2007-06-01

    Xe excimer lamps are used as VUV source for industrial application like surface cleaning. To determine the VUV efficiency of the lamp the radiant flux need to be known. Due to the difficulties of VUV measurements, it is often determined by interpolation from a value of a fixed angle, which results in large uncertainties. Here a goniometric setup is presented to measure the radiant flux of VUV sources like Xe excimer lamps which emit a narrow spectral band in the VUV range between λ = 147 nm and 200 nm with a peak at 172 nm and spectral lines in NIR. By the use of two monochromators, we measure the spectral resolved radiant flux from 120 nm to 1000 nm. The measurement uncertainty of 9.7 % is rather low for the VUV spectral range and depends mainly on the uncertainty of the used deuterium calibration standard from PTB (7%). Due to the strong temperature dependence of the transmission edge of silica used for the lamp vessel, the measurements are done in nitrogen atmosphere to ensure the convection cooling of the lamp. We measured the radiance distribution curve and radiant flux of Xe excimer lamps and could show the angle dependence of the spectrum. The measured correlation between the VUV band and the NIR lines gives us a better understanding of the plasma kinetics, which is used to optimize the pulsed excitation of the lamp.

  10. Comparison of 193 nm and 308 nm laser liquid printing by shadowgraphy imaging

    Science.gov (United States)

    Palla-Papavlu, A.; Shaw-Stewart, J.; Mattle, T.; Dinca, V.; Lippert, T.; Wokaun, A.; Dinescu, M.

    2013-08-01

    Over the last years laser-induced forward transfer has emerged as a versatile and powerful tool for engineering surfaces with active compounds. Soft, easily damageable materials can be transferred using a triazene polymer as a sacrificial layer which acts as a pressure generator and at the same time protects the material from direct laser irradiation. To understand and optimize the transfer process of biomolecules in liquid solution by using an intermediate triazene polymer photosensitive layer, shadowgraphy imaging is carried out. Two laser systems i.e. an ArF laser operating at 193 nm and a XeCl laser operating at 308 nm are applied for the transfer. Solutions with 50% v/v glycerol concentration are prepared and the influence of the triazene polymer sacrificial layer thickness (60 nm) on the deposits is studied. The shadowgraphy images reveal a pronounced difference between laser-induced forward transfer using 193 nm or 308 nm, i.e. very different shapes of the ejected liquid.

  11. Epigenetics and sex differences in the brain: A genome-wide comparison of histone-3 lysine-4 trimethylation (H3K4me3) in male and female mice.

    Science.gov (United States)

    Shen, Erica Y; Ahern, Todd H; Cheung, Iris; Straubhaar, Juerg; Dincer, Aslihan; Houston, Isaac; de Vries, Geert J; Akbarian, Schahram; Forger, Nancy G

    2015-06-01

    Many neurological and psychiatric disorders exhibit gender disparities, and sex differences in the brain likely explain some of these effects. Recent work in rodents points to a role for epigenetics in the development or maintenance of neural sex differences, although genome-wide studies have so far been lacking. Here we review the existing literature on epigenetics and brain sexual differentiation and present preliminary analyses on the genome-wide distribution of histone-3 lysine-4 trimethylation in a sexually dimorphic brain region in male and female mice. H3K4me3 is a histone mark primarily organized as 'peaks' surrounding the transcription start site of active genes. We microdissected the bed nucleus of the stria terminalis and preoptic area (BNST/POA) in adult male and female mice and used ChIP-Seq to compare the distribution of H3K4me3 throughout the genome. We found 248 genes and loci with a significant sex difference in H3K4me3. Of these, the majority (71%) had larger H3K4me3 peaks in females. Comparisons with existing databases indicate that genes and loci with increased H3K4me3 in females are associated with synaptic function and with expression atlases from related brain areas. Based on RT-PCR, only a minority of genes with a sex difference in H3K4me3 has detectable sex differences in expression at baseline conditions. Together with previous findings, our data suggest that there may be sex biases in the use of epigenetic marks. Such biases could underlie sex differences in vulnerabilities to drugs or diseases that disrupt specific epigenetic processes. Copyright © 2014 Elsevier Inc. All rights reserved.

  12. 308-nm excimer laser in endodontics

    Science.gov (United States)

    Liesenhoff, Tim

    1992-06-01

    Root canal preparation was performed on 20 extracted human teeth. After opening the coronal pulp, the root canals were prepared by 308 nm excimer laser only. All root canals were investigated under SEM after separation in the axial direction. By sagittal separation of the mandibles of freshly slaughtered cows, it was possible to get access to the tissues and irradiate under optical control. Under irradiation of excimer laser light, tissue starts to fluoresce. It was possible to demonstrate that each tissue (dentin, enamel, bone, pulpal, and connective tissue) has a characteristic spectral pattern. The SEM analyses showed that it is well possible to prepare root canals safely. All organic soft tissue has been removed by excimer laser irradiation. There was no case of via falsa. The simultaneous spectroscopic identification of the irradiated tissue provides a safe protection from overinstrumentation. First clinical trials on 20 patients suffering of chronical apical parodontitis have been carried out successfully.

  13. Photodissociation of the Propargyl (C3D3) Radicals at 248 nm and 193 nm

    Energy Technology Data Exchange (ETDEWEB)

    Neumark., D.M.; Crider, P.E.; Castiglioni, L.; Kautzman, K.K.

    2009-01-21

    The photodissociation of perdeuterated propargyl (D{sub 2}CCCD) and propynyl (D{sub 3}CCC) radicals was investigated using fast beam photofragment translational spectroscopy. Radicals were produced from their respective anions by photodetachment at 540 nm and 450 nm (below and above the electron affinity of propynyl). The radicals were then photodissociated by 248 nm or 193 nm light. The recoiling photofragments were detected in coincidence with a time- and position-sensitive detector. Three channels were observed: D{sub 2} loss, CD + C{sub 2}D{sub 2}, and CD{sub 3} + C{sub 2}. Obervation of the D loss channel was incompatible with this experiment and was not attempted. Our translational energy distributions for D{sub 2} loss peaked at nonzero translational energy, consistent with ground state dissociation over small (< 1 eV) exit barriers with respect to separated products. Translational energy distributions for the two heavy channels peaked near zero kinetic energy, indicating dissociation on the ground state in the absence of exit barriers.

  14. Design and Simulation of Low Noise Amplifiers at 180nm and 90nm Technologies

    Directory of Open Access Journals (Sweden)

    Fathima Janisha

    2016-11-01

    Full Text Available With continued process scaling, CMOS has become a viable technology for the design of high-performance low noise amplifiers (LNAs in the radio frequency (RF regime. This thesis presents design and simulation of LNA at 180nm and 90nm technology. The LNA function is used to amplify signals without adding noise. The work is done on Cadence Virtuoso platform and the performance parameters like transient response and Noise figure are simulated and plotted. A supply voltage of just 5mV is used here. The noise figure at 180nm is found to be 259.722mdB at 1.04502GHz and The noise figure at 90nm is found to be 183.21mdB at 1.157GHz. 1.04502GHz and 1.157GHz are the peak frequency obtained from the frequency response of the Low noise amplifier. It is observed that the noise figure varies in each technology.

  15. Picosecond Laser Shock Peening of Nimonic 263 at 1064 nm and 532 nm Wavelength

    Directory of Open Access Journals (Sweden)

    Sanja Petronic

    2016-02-01

    Full Text Available The paper presents a study on the surface modifications of nickel based superalloy Nimonic 263 induced by laser shock peening (LSP process. The process was performed by Nd3+:Yttrium Aluminium Garnet (YAG picosecond laser using the following parameters: pulse duration 170 ps; repetition rate 10 Hz; pulse numbers of 50, 100 and 200; and wavelength of 1064 nm (with pulse energy of 2 mJ, 10 mJ and 15 mJ and 532 nm (with pulse energy of 25 mJ, 30 mJ and 35 mJ. The following response characteristics were analyzed: modified surface areas obtained by the laser/material interaction were observed by scanning electron microscopy; elemental composition of the modified surface was evaluated by energy-dispersive spectroscopy (EDS; and Vickers microhardness tests were performed. LSP processing at both 1064 nm and 532 nm wavelengths improved the surface structure and microhardness of a material. Surface morphology changes of the irradiated samples were determined and surface roughness was calculated. These investigations are intended to contribute to the study on the level of microstructure and mechanical properties improvements due to LSP process that operate in a picosecond regime. In particular, the effects of laser wavelength on the microstructural and mechanical changes of a material are studied in detail.

  16. Laser Shock Processing of 6061-T6 Al alloy with 1064 nm and 532 nm wavelengths

    Energy Technology Data Exchange (ETDEWEB)

    Gomez-Rosas, G., E-mail: gomezrg@hotmail.com [Departamento de Fisica, Centro Universitario de Ciencias Exactas e Ingenierias, CUCEI, Universidad de Guadalajara, Blvd. Marcelino Garcia Barragan 1421, Guadalajara, Jalisco 44430 (Mexico); Rubio-Gonzalez, C. [Centro de Ingenieria y Desarrollo Industrial, Pie de la cuesta No. 702, Desarrollo San Pablo, Queretaro, Qro. 76130 (Mexico); Ocana, J.L.; Molpeceres, C.; Porro, J.A.; Morales, M. [Departamento de Fisica Aplicada a la Ingenieria Industrial, ETSII. Universidad Politecnica de Madrid (Spain); Casillas, F.J. [Departamento de Ciencias Exactas y Tecnologicas, Universidad de Guadalajara, Lagos de Moreno, Jalisco 47460 (Mexico)

    2010-08-01

    Laser Shock Processing (LSP) has been proposed as a competitive alternative technology to classical treatments for improving fatigue and wear resistance of metals. We present a configuration and results in the LSP concept for metal surface treatments in underwater laser irradiation at 532 nm and 1064 nm. The purpose of the work is to compare the effect of both wavelengths on the same material. A convergent lens is used to deliver 1.2 J/pulse (1064 nm) and 0.9 J/pulse (532 nm) in a 8 ns laser FWHM pulse produced by 10 Hz Q-switched Nd:YAG laser with spots of a 1.5 mm in diameter moving forward along the work piece. A LSP configuration with experimental results using a pulse density of 2500 pulses/cm{sup 2} and 5000 pulses/cm{sup 2} in 6061-T6 aluminum samples are presented. High level compressive residual stresses are produced using both wavelengths. It has been shown that surface residual stress level is comparable to that achieved by conventional shot peening, but with greater depths. This method can be applied to surface treatment of final metal products.

  17. Characterization of LANDSAT Panels Using the NIST BRDF Scale from 1100 nm to 2500 nm

    Science.gov (United States)

    Markham, Brian; Tsai, Benjamin K.; Allen, David W.; Cooksey, Catherine; Yoon, Howard; Hanssen, Leonard; Zeng, Jinan; Fulton, Linda; Biggar, Stuart; Markham, Brian

    2010-01-01

    Many earth observing sensors depend on white diffuse reflectance standards to derive scales of radiance traceable to the St Despite the large number of Earth observing sensors that operate in the reflective solar region of the spectrum, there has been no direct method to provide NIST traceable BRDF measurements out to 2500 rim. Recent developments in detector technology have allowed the NIST reflectance measurement facility to expand the operating range to cover the 250 nm to 2500 nm range. The facility has been modified with and additional detector using a cooled extended range indium gallium arsenide (Extended InGaAs) detector. Measurements were made for two PTFE white diffuse reflectance standards over the 1100 nm to 2500 nm region at a 0' incident and 45' observation angle. These two panels will be used to support the OLI calibration activities. An independent means of verification was established using a NIST radiance transfer facility based on spectral irradiance, radiance standards and a diffuse reflectance plaque. An analysis on the results and associated uncertainties will be discussed.

  18. The Spectrum of Thorium from 250 nm to 5500 nm: Ritz Wavelengths and Optimized Energy Levels

    CERN Document Server

    Redman, Stephen L; Sansonetti, Craig J

    2013-01-01

    We have made precise observations of a thorium-argon hollow cathode lamp emission spectrum in the region between 350 nm and 1175 nm using a high-resolution Fourier transform spectrometer. Our measurements are combined with results from seven previously published thorium line lists (Giacchetti et al. 1974; Zalubas & Corliss 1974; Zalubas 1976; Palmer & Engleman 1983; Engleman et al. 2003; Lovis & Pepe 2007; Kerber et al. 2008) to re-optimize the energy levels of neutral, singly-, and doubly-ionized thorium (Th I, Th II, and Th III). Using the optimized level values, we calculate accurate Ritz wavelengths for 19679 thorium lines between 250 nm and 5500 nm (40000 1/cm to 1800 1/cm). We have also found 102 new thorium energy levels. A systematic analysis of previous measurements in light of our new results allows us to identify and propose corrections for systematic errors in Palmer & Engleman (1983) and typographical errors and incorrect classifications in Kerber et al. (2008). We also found a la...

  19. Distorted Tetrahedral CoII in K5H[CoW12O40]·xH2O Probed by 2p3d Resonant Inelastic X-ray Scattering

    NARCIS (Netherlands)

    Liu, LIU BY; Wang, Ru Pan; Glass, Elliot N.; Hill, Craig L.; Cuk, Tanja; Okamoto, Jun; Huang, Di Jing; Van Schooneveld, Matti M.; De Groot, Frank M F

    2016-01-01

    The Co 2p3/2 X-ray absorption spectroscopy and high-energy-resolution (∼0.09 eV fwhm) 2p3d resonant inelastic X-ray scattering (RIXS) spectra of the single-cobalt-centered polyoxometalate K5H[CoW12O40]·xH2O were measured. The low-energy dd transition features at 0.55 eV, unmeasurable with

  20. Multicolor immunofluorescence reveals that p63- and/or K5-positive progenitor cells contribute to normal breast epithelium and usual ductal hyperplasia but not to low-grade intraepithelial neoplasia of the breast.

    Science.gov (United States)

    Boecker, Werner; Stenman, Göran; Schroeder, Tina; Schumacher, Udo; Loening, Thomas; Stahnke, Lisa; Löhnert, Catharina; Siering, Robert Michael; Kuper, Arthur; Samoilova, Vera; Tiemann, Markus; Korsching, Eberhard; Buchwalow, Igor

    2017-03-16

    We contend that knowledge about the cellular composition of normal breast epithelium is a prerequisite for understanding proliferative breast disease. Against this background, we used multicolor immunofluorescence to study normal breast epithelium and two types of intraepithelial proliferative breast lesion for expression of the p63, basal keratin K5, glandular keratin K8/18, SMA, ER-alpha, and Ki67. We studied eight normal breast epithelium samples, 12 cases of usual ductal hyperplasia, and 33 cases of low-grade intraepithelial neoplasia (9 flat epithelial atypia, 14 low-grade ductal carcinoma in situ and 10 cases of lobular neoplasia). Usual ductal hyperplasia showed striking similarity to normal luminal breast epithelium including p63+ and/or K5+ luminal progenitor cells and the full spectrum of luminal progeny cells. In normal breast epithelium and usual ductal hyperplasia, expression of ER-alpha was associated with lack of expression of the proliferation antigen Ki67. In contrast, we found in both types of low-grade intraepithelial neoplasia robust expression of keratin K8/18 and a positive association between ER-alpha and Ki67 expression. However, these lesions were consistently negative for p63 and/or K5. Our observational study supports the view that usual ductal hyperplasia and low-grade intraepithelial neoplasia are different entities rather than part of a spectrum of the same disease. We propose a new operational model of cell differentiation that may serve to better understand correlations between normal breast epithelium and proliferative breast diseases. From our data we conclude that p63+ and/or K5+ progenitor cells contribute to maintenance of normal epithelium and usual ductal hyperplasia, but not to low-grade intraepithelial neoplasia of the breast.

  1. k482ar.m77t - MGD77 data file for Geophysical data from field activity K-4-82-AR in Arctic from 09/13/1982 to 10/10/1982

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Single-beam bathymetry data along with DGPS navigation data was collected as part of field activity K-4-82-AR in Arctic from 09/13/1982 to 10/10/1982,...

  2. Half Swing Clocking Scheme at 45nm

    Directory of Open Access Journals (Sweden)

    Sakshi Verma,

    2014-01-01

    Full Text Available Achievement of high processor speed with low power consumption is an elemental factor in processor technology, especially for hand-held devices. The need for low power has caused a major paradigm shift where power dissipation has become a important consideration as performance and area. In CMOS circuits, dynamic power consumption is proportional to the transition frequency, capacitance, and square of supply voltage. Consequentially, lowering supply voltage delivers significant power savings compromising the speed of processor. Large portion of the total power is consumed in the clocking circuitry in embedded processor technology. So clock power can be reduced using half swing of clock scheme which will cut down the power dissipation and minimum speed degradation. In Digital circuits by using double-edge triggered flip flops (DETFFs, the clock frequency can be significantly reduced ideally, in half while preserving the rate of data processing. Using lower clock frequency may translate into considerable power savings for the clocked portions of a circuit, including the clock distribution network and flip-flops. The designing is based on 45nm process technology.

  3. Measurement of the Energy Expenditures in Daily Physical Activities with K4b2%应用K4b2心肺功能测定仪对日常体力活动能量消耗的测定

    Institute of Scientific and Technical Information of China (English)

    刘健敏; 李颜; 杨晓光

    2010-01-01

    应用K4b2心肺功能测定仪对日常体力活动的能量消耗进行现场测定,以期为我国体力活动指南的制定提供基础资料.选取23名北医青年学生作为受试者,其中男11名,女12名,应用K4b2心肺功能测定仪对受试者在静息、慢走(3 km/h)、快走(6 km/h)、慢跑(8 km/h)、骑自行车(12 km/h)状态下的能量消耗进行测定.各项体力活动的能量消耗及代谢当量由大到小排列,依次为幔跑((6.26±1.22)kcal·kg-1~·h-1,6.0 MET)、骑自行车((4.30±0.67)kcal·kg-1·h-1,4.2 MET)、快走((3.75±0.73)kcal·ks-1·h-1,3.6 MET)、慢走((1.24±0.26)kcal·kg-1·h-1,1.2 MET)、静息((1.08±0.24)kcal·kg-1·h-1,1.0 MET),按照体力活动强度分级,属于中低强度体力活动.慢走、快走、慢跑、骑自行车等日常体力活动适于在中国人群中推广.

  4. Multi-watt 589nm fiber laser source

    Energy Technology Data Exchange (ETDEWEB)

    DAWSON, J W; DROBSHOFF, A D; BEACH, R J; MESSERLY, M J; PAYNE, S A; BROWN, A; PENNINGTON, D M; BAMFORD, D J; SHARPE, S J; COOK, D J

    2006-01-19

    We have demonstrated 3.5W of 589nm light from a fiber laser using periodically poled stoichiometric Lithium Tantalate (PPSLT) as the frequency conversion crystal. The system employs 938nm and 1583nm fiber lasers, which were sum-frequency mixed in PPSLT to generate 589nm light. The 938nm fiber laser consists of a single frequency diode laser master oscillator (200mW), which was amplified in two stages to >15W using cladding pumped Nd{sup 3+} fiber amplifiers. The fiber amplifiers operate at 938nm and minimize amplified spontaneous emission at 1088nm by employing a specialty fiber design, which maximizes the core size relative to the cladding diameter. This design allows the 3-level laser system to operate at high inversion, thus making it competitive with the competing 1088nm 4-level laser transition. At 15W, the 938nm laser has an M{sup 2} of 1.1 and good polarization (correctable with a quarter and half wave plate to >15:1). The 1583nm fiber laser consists of a Koheras 1583nm fiber DFB laser that is pre-amplified to 100mW, phase modulated and then amplified to 14W in a commercial IPG fiber amplifier. As a part of our research efforts we are also investigating pulsed laser formats and power scaling of the 589nm system. We will discuss the fiber laser design and operation as well as our results in power scaling at 589nm.

  5. Absolute frequency references at 1529 nm and 1560 nm using modulation transfer spectroscopy

    CERN Document Server

    de Escobar, Y Natali Martinez; Coop, Simon; Vanderbruggen, Thomas; Kaczmarek, Krzysztof T; Mitchell, Morgan W

    2015-01-01

    We demonstrate a double optical frequency reference (1529 nm and 1560 nm) for the telecom C-band using $^{87}$Rb modulation transfer spectroscopy. The two reference frequencies are defined by the 5S$_{1/2} F=2 \\rightarrow $ 5P$_{3/2} F'=3$ two-level and 5S$_{1/2} F=2 \\rightarrow $ 5P$_{3/2} F'=3 \\rightarrow $ 4D$_{5/2} F"=4$ ladder transitions. We examine the sensitivity of the frequency stabilization to probe power and magnetic field fluctuations, calculate its frequency shift due to residual amplitude modulation, and estimate its shift due to gas collisions. The short-term Allan deviation was estimated from the error signal slope for the two transitions. Our scheme provides a simple and high performing system for references at these important wavelengths. We estimate an absolute accuracy of $\\sim$ 1 kHz is realistic.

  6. Magnetic Susceptibility of liquid Gd-NM (NM = Cu, Ga, Ge) alloys

    Science.gov (United States)

    Shimakura, Hironori; Tahara, Shuta; Okada, Tatsuya; Ohno, Satoru

    2017-08-01

    For rare earth alloys, the indirect interaction of RKKY is at work between rare-earth atoms. Therefore, the magnetism of them depends on the number of conduction electrons and the distance between rare-earth metals. In this work, to reveal the relationship between the number of conduction electrons and magnetic property of rare earth metal alloys, magnetic susceptibility measurements for liquid Gd-NM (NM = Cu, Ga, Ge) was performed by Faraday method. As the results, it was observed that the sign of paramagnetic Curie temperature of Cu-Gd alloys are positive at all composition, while Ga-Gd and Ge-Gd alloys show negative paramagnetic Curie temperature at certain composition. Moreover, it was indicated when the alloy at certain composition shows highest melting temperature, it has the lowest paramagnetic Curie temperature.

  7. Solar irradiance models and measurements: a comparison in the 220 nm to 240 nm wavelength band

    CERN Document Server

    Unruh, Yvonne C; Krivova, Natalie A

    2011-01-01

    Solar irradiance models that assume solar irradiance variations to be due to changes in the solar surface magnetic flux have been successfully used to reconstruct total solar irradiance on rotational as well as cyclical and secular time scales. Modelling spectral solar irradiance is not yet as advanced, and also suffers from a lack of comparison data, in particular on solar-cycle time scales. Here we compare solar irradiance in the 220 nm to 240 nm band as modelled with SATIRE-S and measured by different instruments on the UARS and SORCE satellites. We find good agreement between the model and measurements on rotational time scales. The long-term trends, however, show significant differences. Both SORCE instruments, in particular, show a much steeper gradient over the decaying part of cycle 23 than the modelled irradiance or that measured by UARS/SUSIM.

  8. Analysis of multi-mode to single-mode conversion at 635 nm and 1550 nm

    Science.gov (United States)

    Zamora, Vanessa; Bogatzki, Angelina; Arndt-Staufenbiel, Norbert; Hofmann, Jens; Schröder, Henning

    2016-03-01

    We propose two low-cost and robust optical fiber systems based on the photonic lantern (PL) technology for operating at 635 nm and 1550 nm. The PL is an emerging technology that couples light from a multi-mode (MM) fiber to several single-mode (SM) fibers via a low-loss adiabatic transition. This bundle of SM fibers is observed as a MM fiber system whose spatial modes are the degenerate supermodes of the bundle. The adiabatic transition allows that those supermodes evolve into the modes of the MM fiber. Simulations of the MM fiber end structure and its taper transition have been performed via functional mode solver tools in order to understand the modal evolution in PLs. The modelled design consists of 7 SM fibers inserted into a low-index capillary. The material and geometry of the PLs are chosen such that the supermodes match to the spatial modes of the desired step-index MM fiber in a moderate loss transmission. The dispersion of materials is also considered. These parameters are studied in two PL systems in order to reach a spectral transmission from 450 nm to 1600 nm. Additionally, an analysis of the geometry and losses due to the mismatching of modes is presented. PLs are typically used in the fields of astrophotonics and space photonics. Recently, they are demonstrated as mode converters in telecommunications, especially focusing on spatial division multiplexing. In this study, we show the use of PLs as a promising interconnecting tool for the development of miniaturized spectrometers operating in a broad wavelength range.

  9. TUNABLE DIODE LASER MEASUREMENTS OF NO2 NEAR 670 NM AND 395 NM. (R823933)

    Science.gov (United States)

    Two single-mode diode lasers were used to record high-resolution absorption spectra of NO2 (dilute in Ar) near 670.2 and 394.5 nm over a range of temperatures (296 to 774 K) and total pressures (2.4 x 10(-2) to 1 atm). A commercial InGaAsP laser was tuned 1.3 cm(-1) at a repetiti...

  10. Monolithic PM Raman fiber laser at 1679 nm for Raman amplification at 1810 nm

    DEFF Research Database (Denmark)

    Svane, Ask Sebastian; Rottwitt, Karsten

    2013-01-01

    Stimulated Raman scattering (SRS) has been subject to much attention within the field of fiber lasers and amplifiers as it provides an extended wavelength coverage in comparison to rare-earth based devices. Motivated by the projected capacity crunch [1], different approaches are being explored...... demonstrate a monolithic RM Raman fiber laser (RFL), which acts as a pump for a Raman amplifier (RA) at 1810 nm. The lasing wavelength of a RFL, thus also for a RA, can in principle be designed arbitrarily within the entire wavelength range from the Erbium band up to the Thulium/Holmium band...... of OFS PM Raman fiber, with an estimated propagation loss of 0.42/0.46/1.3 dB/km at 1564/1679/1810 nm. The Raman gain coefficient was measured to be gR=2.66/2.35 W-1km-1 at 1679/1810 nm. The laser curve of the RFL is depicted in Fig. 1b, with a slope efficiency of 67 %. The high slope efficiency...

  11. High-resolution photoabsorption cross section measurements of sulfur dioxide between 198 nm and 325 nm

    Science.gov (United States)

    Stark, Glenn; Smith, Peter; Blackie, Douglas; Blackwell-Whitehead, Richard; Pickering, Juliet; Rufus, James; Thorne, Anne

    Accurate photoabsorption cross section data at a range of temperatures are required for the incorporation of sulfur dioxide into atmospheric photochemical models. In addition to its role in the terrestrial atmosphere, sulfur dioxide is observed in significant concentrations in the atmospheres of Venus and Io. Our laboratory measurement program focuses on the very congested SO2 spectrum in the ultraviolet. Using the Imperial College UV Fourier transform spectrometer, we have recorded high-resolution (resolving power (λ/∆λ) = 450,000) absorption spectra in the 198 to 325 nm region over a range of temperatures from 160 K to 295 K. This high resolving power allows resolutions approaching those required to fully resolve the Doppler profile of SO2 in the UV. We have reported absolute photoabsorption cross sections at 295 K [Stark et al., JGR Planets 104, 16585 (1999); Rufus et al. JGR Planets 108, doi:10.1029/2002JE001931,(2003)]. Further measurements, at 160 K in the 198 to 200 nm region and at 195 K in the 220 to 325 nm region, have been recorded and analyzed. We present an overview of our new measured cross sections at temperatures and pressures comparable to those found in planetary atmospheres. This work was supported in part by NASA Grant NNG05GA03G, PPARC (UK), and the Leverhulme Trust.

  12. Generation of a 650 nm - 2000 nm Laser Frequency Comb based on an Erbium-Doped Fiber Laser

    CERN Document Server

    Ycas, Gabriel; Diddams, Scott A

    2012-01-01

    We present a laser frequency comb based upon a 250 MHz mode-locked erbium-doped fiber laser that spans more than 300 terahertz of bandwidth, from 660 nm to 2000 nm. The system generates 1.2 nJ, 70 fs pulses at 1050 nm by amplifying the 1580 nm laser light in Er:fiber, followed by nonlinear broadening to 1050 nm and amplification in Yb:fiber. Extension of the frequency comb into the visible is achieved by supercontinuum generation from the 1050 nm light. Comb coherence is verified with cascaded f-2f interferometry and comparison to a frequency stabilized laser.

  13. Performance comparison of bismuth/erbium co-doped optical fibre by 830 nm and 980 nm pumping

    Science.gov (United States)

    Yan, Binbin; Luo, Yanhua; Zareanborji, Amirhassan; Xiao, Gui; Peng, Gang-Ding; Wen, Jianxiang

    2016-10-01

    The performance of bismuth/erbium co-doped fibre (BEDF) by 830 nm and 980 nm pumping has been studied in detail, including the small signal absorption, pump absorption, emission, gain and excited state absorption (ESA). Based on the study, energy transition diagrams of BEDF under 830 nm or 980 nm pumping are proposed to clarify the spectroscopic properties. The results demonstrate the advantages of 830 nm pumping for BEDF over 980 nm pumping when considering the absorption, pumping efficiency, excited state absorption and optical amplification.

  14. 76 FR 22015 - Amendment of Class E Airspace; Raton, NM

    Science.gov (United States)

    2011-04-20

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Raton, NM AGENCY: Federal... Raton, NM, to accommodate new Area Navigation (RNAV) Standard Instrument Approach Procedures at Raton Municipal Airport/Crews Field, Raton, NM. The FAA is taking this action to enhance the safety and...

  15. The influence of agitation sequence and ionic strength on in vitro drug release from hypromellose (E4M and K4M) ER matrices--the use of the USP III apparatus.

    Science.gov (United States)

    Asare-Addo, Kofi; Kaialy, Waseem; Levina, Marina; Rajabi-Siahboomi, Ali; Ghori, Mohammed U; Supuk, Enes; Laity, Peter R; Conway, Barbara R; Nokhodchi, Ali

    2013-04-01

    Theophylline extended release (ER) matrices containing hypromellose (hydroxypropyl methylcellulose (HPMC) E4M and K4M were evaluated in media with a pH range of 1.2-7.5, using an automated USP type III, Bio-Dis dissolution apparatus. The objectives of this study were to evaluate the effects of systematic agitation, ionic strength and pH on the release of theophylline from the gel forming hydrophilic polymeric matrices with different methoxyl substitution levels. Tribo-electric charging of hypromellose, theophylline and their formulated blends containing E4M and K4M grades has been characterised, along with quantitative observations of flow, compression behaviour and particle morphology. Agitations were studied at 5, 10, 15, 20, 25, 30 dips per minute (dpm) and also in the ascending and descending order in the dissolution vials. The ionic concentration strength of the media was also varied over a range of 0-0.4M to simulate the gastrointestinal fed and fasted states and various physiological pH conditions. To study the effect of ionic strength on the hydrophilic matrices, agitation was set at 20 dpm. The charge results on individual components imply that the positively charged particles have coupled with the negatively charged particles to form a stable ordered mixture which is believed to result in a more homogeneous and stable system. The particle shape analysis showed the HPMC K4M polymer to have a more irregular morphology and a rougher surface texture in comparison to the HPMC E4M polymer, possibly a contributory factor to the gelation process. The results showed gelation occurred quicker for the K4M tablet matrices. Drug release increased with increased agitation. This was more pronounced for the E4M tablet matrices. The ionic strength also had more of an effect on the drug release from the E4M matrices. The experiments highlighted the resilience of the K4M matrices in comparison with the E4M matrices. The results thus show that despite similar viscosities of

  16. From the nm to the Mm

    Science.gov (United States)

    Villa, I. M.

    2003-12-01

    Tectonic models for the evolution of an orogen start at the Mm scale, and use field work on smaller subunits at the km scale and rocks collected at the m scale. At the mm scale, minerals are identified, analyzed by mass spectrometry, their "cooling ages" assigned to a specific closure temperature, a cooling rate attributed to a particular tectonic regime, and a large body of self-referential literature is the product of an oiled machinery. Problems become apparent if one attempts to harmonize mm-scale science with the nasty little details at even smaller scales. Atoms are invisible to the naked eye (unlike the minerals mentioned above) and their actual behavior is, or was, only accessible to indirect argumentations and simplified calculations. Increased computing power now allows calculating the transport of atoms in a crystal from the Schr”dinger equation: results do not fit 19th century continuum physics for infinitely dilute solutions (Fick's and Arrhenius' "laws"). Moreover, improved nanochemical analyses allow characterizing the supposedly homogeneous mineral matrix. TEM images show how layers or chains in pristine minerals are substituted in a non-periodic way by alteration products. EMP analyses show the almost ubiquitous presence of razor-sharp boundaries rather than Erf profiles. Disequilibrium recrystallization textures thus prevail over diffusive reequilibration; diffusion sensu stricto is shown to be a much slower process than heterochemical replacement. Alterability sequences are well known to surface scientists: e.g. halite, olivine, biotite, muscovite, zircon. Such sequences are reflected in the isotopic retentivity. The link only becomes clear at the nm scale: isotopic exchange occurs during the replacement reactions that affect all rocks on their retrograde P-T evolution. This is sufficient to explain why zircons record higher isotopic ages than muscovites, which in turn undergo less isotope exchange than biotites etc. While there is a vague

  17. Cell projection use in maskless lithography for 45nm and 32nm logic nodes

    Science.gov (United States)

    Manakli, S.; Komami, H.; Takizawa, M.; Mitsuhashi, T.; Pain, L.

    2009-03-01

    Due to the ever-increasing cost of equipment and mask complexity, the use of optical lithography for integrated circuit manufacturing is increasingly more complex and expensive. Recent workshops and conferences in semiconductor lithography underlined that one alternative to support sub-32nm technologies is mask-less lithography option using electron beam technology. However, this direct write approach based on variable shaped beam principle (VSB) is not sufficient in terms of throughput, i.e. of productivity. New direct write techniques like multibeam systems are under development, but these solutions will not be mature before 2012. The use of character/cell projection (CP) on industrial VSB tools is the first step to deal with the throughput concerns. This paper presents the status of the CP technology and evaluates its possible use for the 45nm and 32nm logic nodes. It will present standard cell and SRAM structures that are printed as single characters using the CP technique. All experiments are done using the Advantest tool (F3000) which can project up to 100 different cells per layer. Cell extractions and design have been performed with the design and software solution developed by D2S. In this paper, we first evaluate the performance gain that can be obtained with the CP approach compared to the standard VSB approach. This paper also details the patterning capability obtained by using the CP concept. An evaluation of the CD uniformity and process stability is also presented. Finally this paper discusses about the improvements of this technique to address high resolution and to improve the throughput concerns.

  18. Coordinated bifluoride ions in the first thiofluoride molybdenum triangular cluster complex: synthesis and crystal structure of K5[Mo3S4F7(FHF)2]·2H2O

    Science.gov (United States)

    Mironov, Yu. V.; Yarovoi, S. S.; Solodovnikov, S. F.; Fedorov, V. E.

    2003-08-01

    The first triangular thiofluoride cluster complex of molybdenum K5[Mo3S4F7(FHF)2]·2H2O with unexpected coordination of bifluoride ions has been synthesized by the reaction of Mo3S7Br4 with molten KHF2. The compound was characterized by single crystal X-ray diffraction. The compound crystallizes in the orthorhombic space group Cmc21 with four formulas in unit cell of dimensions a=15.1060(20) Å,b=8.7820(9) Å,c=14.3358(13) Å,V=1901.8(4) Å3.

  19. Manufacturability of 2x-nm devices with EUV tool

    Science.gov (United States)

    Tawarayama, Kazuo; Nakajima, Yumi; Kyoh, Suigen; Aoyama, Hajime; Matsunaga, Kentaro; Magoshi, Shunko; Tanaka, Satoshi; Hayashi, Yumi; Mori, Ichiro

    2011-04-01

    Due to the promising development status of EUVL as a practical lithography technology for the 2x-nm node, we are continuing to evaluate its process liability using the EUV1 at Selete, which has an Off-Axis illumination capability. The resolution limit of the EUV1 for L&S patterns is currently 18 nm for dipole illumination, and 16 nm for aggressive dipole illumination. This study examined the critical points of EUVL for device manufacturing through wafer processes. The yield obtained from electrical measurements indicates the maturity of the technology, including the resist process, the tool, and the mask. Optimization of the resist and RIE processes significantly improved the yield. The final yields obtained from electrical measurements were 100% for hp 30 nm, 70% for hp 28 nm, and 40% for hp 26 nm. These results demonstrate EUV lithography to be a practical technology that is now suitable for 2x nm semiconductor manufacture.

  20. Ultraviolet 320 nm laser excitation for flow cytometry.

    Science.gov (United States)

    Telford, William; Stickland, Lynn; Koschorreck, Marco

    2017-04-01

    Although multiple lasers and high-dimensional analysis capability are now standard on advanced flow cytometers, ultraviolet (UV) lasers (usually 325-365 nm) remain an uncommon excitation source for cytometry. This is primarily due to their cost, and the small number of applications that require this wavelength. The development of the Brilliant Ultraviolet (BUV fluorochromes, however, has increased the importance of this formerly niche excitation wavelength. Historically, UV excitation was usually provided by water-cooled argon- and krypton-ion lasers. Modern flow cytometers primary rely on diode pumped solid state lasers emitting at 355 nm. While useful for all UV-excited applications, DPSS UV lasers are still large by modern solid state laser standards, and remain very expensive. Smaller and cheaper near UV laser diodes (NUVLDs) emitting at 375 nm make adequate substitutes for 355 nm sources in many situations, but do not work as well with very short wavelength probes like the fluorescent calcium chelator indo-1. In this study, we evaluate a newly available UV 320 nm laser for flow cytometry. While shorter in wavelength that conventional UV lasers, 320 is close to the 325 nm helium-cadmium wavelength used in the past on early benchtop cytometers. A UV 320 nm laser was found to excite almost all Brilliant Ultraviolet dyes to nearly the same level as 355 nm sources. Both 320 nm and 355 nm sources worked equally well for Hoechst and DyeCycle Violet side population analysis of stem cells in mouse hematopoetic tissue. The shorter wavelength UV source also showed excellent excitation of indo-1, a probe that is not compatible with NUVLD 375 nm sources. In summary, a 320 nm laser module made a suitable substitute for conventional 355 nm sources. This laser technology is available in a smaller form factor than current 355 nm units, making it useful for small cytometers with space constraints. © 2017 International Society for Advancement of Cytometry. © 2017 International

  1. GmPHD5 acts as an important regulator for crosstalk between histone H3K4 di-methylation and H3K14 acetylation in response to salinity stress in soybean

    Directory of Open Access Journals (Sweden)

    Wu Tao

    2011-12-01

    Full Text Available Abstract Background Accumulated evidence suggest that specific patterns of histone posttranslational modifications (PTMs and their crosstalks may determine transcriptional outcomes. However, the regulatory mechanisms of these "histone codes" in plants remain largely unknown. Results In this study, we demonstrate for the first time that a salinity stress inducible PHD (plant homeodomain finger domain containing protein GmPHD5 can read the "histone code" underlying the methylated H3K4. GmPHD5 interacts with other DNA binding proteins, including GmGNAT1 (an acetyl transferase, GmElongin A (a transcription elongation factor and GmISWI (a chromatin remodeling protein. Our results suggest that GmPHD5 can recognize specific histone methylated H3K4, with preference to di-methylated H3K4. Here, we illustrate that the interaction between GmPHD5 and GmGNAT1 is regulated by the self-acetylation of GmGNAT1, which can also acetylate histone H3. GmGNAT1 exhibits a preference toward acetylated histone H3K14. These results suggest a histone crosstalk between methylated H3K4 and acetylated H3K14. Consistent to its putative roles in gene regulation under salinity stress, we showed that GmPHD5 can bind to the promoters of some confirmed salinity inducible genes in soybean. Conclusion Here, we propose a model suggesting that the nuclear protein GmPHD5 is capable of regulating the crosstalk between histone methylation and histone acetylation of different lysine residues. Nevertheless, GmPHD5 could also recruit chromatin remodeling factors and transcription factors of salt stress inducible genes to regulate their expression in response to salinity stress.

  2. FOXP3 Orchestrates H4K16 Acetylation and H3K4 Tri-Methylation for Activation of Multiple Genes through Recruiting MOF and Causing Displacement of PLU-1

    Science.gov (United States)

    Katoh, Hiroto; Qin, Zhaohui S.; Liu, Runhua; Wang, Lizhong; Li, Weiquan; Li, Xiangzhi; Wu, Lipeng; Du, Zhanwen; Lyons, Robert; Liu, Chang-Gong; Liu, Xiuping; Dou, Yali; Zheng, Pan; Liu, Yang

    2011-01-01

    SUMMARY Both H4K16 acetylation and H3K4 tri-methylation are required for gene activation. However, it is still largely unclear how these modifications are orchestrated by transcriptional factors. Here we analyzed the mechanism of the transcriptional activation by FOXP3, an X-linked suppressor of autoimmune diseases and cancers. FOXP3 binds near transcriptional start sites of its target genes. By recruiting MOF and displacing histone H3K4 demethylase PLU-1, FOXP3 increases both H4K16 acetylation and H3K4 tri-methylation at the FOXP3-associated chromatins of multiple FOXP3-activated genes. RNAi-mediated silencing of MOF reduced both gene activation and tumor suppression by FOXP3, while both somatic mutations in clinical cancer samples and targeted mutation of FOXP3 in mouse prostate epithelial disrupted nuclear localization of MOF. Our data demonstrate a pull-push model in which a single transcription factor orchestrates two epigenetic alterations necessary for gene activation and provide a mechanism for somatic inactivation of the FOXP3 protein function in cancer cells. PMID:22152480

  3. Measurements of Stokes parameters of materials at 1064-nm and 532-nm wavelengths

    Science.gov (United States)

    Tan, Songxin; Narayanan, Ram M.; Kalshoven, James E., Jr.

    2001-09-01

    Laser radar systems have found wide applications in the field of remote sensing. Reflectance as well as polarization features are used together for applications ranging from environmental monitoring to target classification. The Stokes parameters are ideal quantities for characterizing the above features because they provide useful information on both light intensity and polarization state. The University of Nebraska is currently refurbishing an airborne multi-wavelength laser radar system based on the NASA Goddard Space Flight Center (GSFC) developed Airborne Laser Polarimetric Sensor (ALPS). The system uses a Nd:YAG laser operating at wavelengths of 1064 nm and 532 nm, and contains four channels at each wavelength to measure the polarization states. This system was used to measure the Stokes parameters of backscattered laser light from different materials. These included canvas tarp, white paper, plywood, concrete, aluminum plate and anodized aluminum plate. The data provide an understanding of the polarized scattering properties of various materials, and are expected to be useful in developing target discrimination algorithms.

  4. 9nm node wafer defect inspection using three-dimensional scanning, a 405nm diode laser, and a broadband source

    Science.gov (United States)

    Zhou, Renjie; Edwards, Chris; Bryniarski, Casey A.; Popescu, Gabriel; Goddard, Lynford L.

    2015-03-01

    We recently built a 405nm laser based optical interferometry system for 9nm node patterned wafer defect inspection. Defects with volumes smaller than 15nm by 90nm by 35nm have been detected. The success of defect detection relied on accurate mechanical scanning of the wafer and custom engineered image denoising post-processing. To further improve the detection sensitivity, we designed a higher precision XYZ scanning stage and replaced the laser source with an incoherent LED to remove the speckle noise. With these system modifications, we successfully detected both defects and surface contamination particles in bright-field imaging mode. Recently, we have upgraded this system for interferometric defect inspection.

  5. Comparison between HMME mediated photodynamic therapy using 413nm and 532nm for port wine stains: a mathematical simulation study

    Science.gov (United States)

    Wang, Y.; Gu, Y.; Chen, R.; Xu, L. Q.; Liao, X. H.; Huang, N. Y.; Wang, Y. Y.

    2007-11-01

    Introduction: As it is always difficult to find the optimal combination of photosensitizer and of laser wavelength to achieve selective vascular damage in PWS-PDT, the selective vascular effects of HMME (Hematoporphyrin monomethyl ether) mediated PDT with 413 nm and with 532 nm were compared by mathematical simulation in this study. Materials & Methods: Firstly, distribution of 413 nm, 532 nm light in PWS tissue was simulated by Monte Carlo model. Two energy density groups were set, one is 80mW/cm2x40min for both 413 nm and 532 nm, the other is 80mW/cm2x40min for 532 nm while 80mW/cm2x20min in for 413 nm. Secondly, the productivity of reactive oxygen species (ROS) in target vessels and normal tissue were simulated using a simulation system for PDT of PWS established in our lab, which considering the amount of light and photosensitizer in tissue, the molar extinction coefficient of photosensitizer, and quantum yield of ROS. Concentration of HMME for each wavelength were same. Finally, the productivity of ROS n in target vessels and normal tissue were compared between 413 nm PDT and 532 nm PDT under different energy density. Result: Under the same energy density, ROS productivity in target vessels of 413 nm PDT was significantly higher than that of 532 nm PDT. Moreover, it was still higher at low energy density than that of 532nm PDT with high energy density. Conclusion: HMME mediated PDT using 413 nm has the potential to increase the selective vascular effect of PDT for PWS by shortening treatment time.

  6. Arabidopsis AL PHD-PRC1 complexes promote seed germination through H3K4me3-to-H3K27me3 chromatin state switch in repression of seed developmental genes.

    Science.gov (United States)

    Molitor, Anne Marie; Bu, Zhongyuan; Yu, Yu; Shen, Wen-Hui

    2014-01-01

    Seed germination and subsequent seedling growth define crucial steps for entry into the plant life cycle. For those events to take place properly, seed developmental genes need to be silenced whereas vegetative growth genes are activated. Chromatin structure is generally known to play crucial roles in gene transcription control. However, the transition between active and repressive chromatin states during seed germination is still poorly characterized and the underlying molecular mechanisms remain largely unknown. Here we identified the Arabidopsis PHD-domain H3K4me3-binding ALFIN1-like proteins (ALs) as novel interactors of the Polycomb Repressive Complex 1 (PRC1) core components AtBMI1b and AtRING1a. The interactions were confirmed by diverse in vitro and in vivo assays and were shown to require the AL6 N-terminus containing PAL domain conserved in the AL family proteins and the AtRING1a C-terminus containing RAWUL domain conserved in animal and plant PRC1 ring-finger proteins (including AtRNIG1a/b and AtBMI1a/b). By T-DNA insertion mutant analysis, we found that simultaneous loss of AL6 and AL7 as well as loss of AtBMI1a and AtBMI1b retards seed germination and causes transcriptional derepression and a delayed chromatin state switch from H3K4me3 to H3K27me3 enrichment of several seed developmental genes (e.g. ABI3, DOG1, CRU3, CHO1). We found that AL6 and the PRC1 H3K27me3-reader component LHP1 directly bind at ABI3 and DOG1 loci. In light of these data, we propose that AL PHD-PRC1 complexes, built around H3K4me3, lead to a switch from the H3K4me3-associated active to the H3K27me3-associated repressive transcription state of seed developmental genes during seed germination. Our finding of physical interactions between PHD-domain proteins and PRC1 is striking and has important implications for understanding the connection between the two functionally opposite chromatin marks: H3K4me3 in activation and H3K27me3 in repression of gene transcription.

  7. Arabidopsis AL PHD-PRC1 complexes promote seed germination through H3K4me3-to-H3K27me3 chromatin state switch in repression of seed developmental genes.

    Directory of Open Access Journals (Sweden)

    Anne Marie Molitor

    2014-01-01

    Full Text Available Seed germination and subsequent seedling growth define crucial steps for entry into the plant life cycle. For those events to take place properly, seed developmental genes need to be silenced whereas vegetative growth genes are activated. Chromatin structure is generally known to play crucial roles in gene transcription control. However, the transition between active and repressive chromatin states during seed germination is still poorly characterized and the underlying molecular mechanisms remain largely unknown. Here we identified the Arabidopsis PHD-domain H3K4me3-binding ALFIN1-like proteins (ALs as novel interactors of the Polycomb Repressive Complex 1 (PRC1 core components AtBMI1b and AtRING1a. The interactions were confirmed by diverse in vitro and in vivo assays and were shown to require the AL6 N-terminus containing PAL domain conserved in the AL family proteins and the AtRING1a C-terminus containing RAWUL domain conserved in animal and plant PRC1 ring-finger proteins (including AtRNIG1a/b and AtBMI1a/b. By T-DNA insertion mutant analysis, we found that simultaneous loss of AL6 and AL7 as well as loss of AtBMI1a and AtBMI1b retards seed germination and causes transcriptional derepression and a delayed chromatin state switch from H3K4me3 to H3K27me3 enrichment of several seed developmental genes (e.g. ABI3, DOG1, CRU3, CHO1. We found that AL6 and the PRC1 H3K27me3-reader component LHP1 directly bind at ABI3 and DOG1 loci. In light of these data, we propose that AL PHD-PRC1 complexes, built around H3K4me3, lead to a switch from the H3K4me3-associated active to the H3K27me3-associated repressive transcription state of seed developmental genes during seed germination. Our finding of physical interactions between PHD-domain proteins and PRC1 is striking and has important implications for understanding the connection between the two functionally opposite chromatin marks: H3K4me3 in activation and H3K27me3 in repression of gene transcription.

  8. Segmentation of the macular choroid in OCT images acquired at 830nm and 1060nm

    Science.gov (United States)

    Lee, Sieun; Beg, Mirza F.; Sarunic, Marinko V.

    2013-06-01

    Retinal imaging with optical coherence tomography (OCT) has rapidly advanced in ophthalmic applications with the broad availability of Fourier domain (FD) technology in commercial systems. The high sensitivity afforded by FD-OCT has enabled imaging of the choroid, a layer of blood vessels serving the outer retina. Improved visualization of the choroid and the choroid-sclera boundary has been investigated using techniques such as enhanced depth imaging (EDI), and also with OCT systems operating in the 1060-nm wavelength range. We report on a comparison of imaging the macular choroid with commercial and prototype OCT systems, and present automated 3D segmentation of the choroid-scleral layer using a graph cut algorithm. The thickness of the choroid is an important measurement to investigate for possible correlation with severity, or possibly early diagnosis, of diseases such as age-related macular degeneration.

  9. 9nm node wafer defect inspection using visible light

    Science.gov (United States)

    Zhou, Renjie; Edwards, Chris; Popescu, Gabriel; Goddard, Lynford L.

    2014-04-01

    Over the past 2 years, we have developed a common optical-path, 532 nm laser epi-illumination diffraction phase microscope (epi-DPM) and successfully applied it to detect different types of defects down to 20 by 100 nm in a 22nm node intentional defect array (IDA) wafer. An image post-processing method called 2DISC, using image frame 2nd order differential, image stitching, and convolution, was used to significantly improve sensitivity of the measured images. To address 9nm node IDA wafer inspection, we updated our system with a highly stable 405 nm diode laser. By using the 2DISC method, we detected parallel bridge defects in the 9nm node wafer. To further enhance detectability, we are exploring 3D wafer scanning, white-light illumination, and dark-field inspection.

  10. Generation of 35.5-nm coherent radiation.

    Science.gov (United States)

    Bokor, J; Bucksbaum, P H; Freeman, R R

    1983-04-01

    Tunable coherent radiation was produced at 35.5 nm by seventh-harmonic conversion of 248-nm radiation from a krypton fluoride excimer laser. The nonlinear interaction took place at the intersection of the laser focus and a pulsed, supersonic helium gas jet. Third- and fifth-harmonic generation produced coherent outputs at 83 and 50 nm in both helium and xenon gas jets.

  11. Detection limits of 405 nm and 633 nm excited PpIX fluorescence for brain tumor detection during stereotactic biopsy

    Science.gov (United States)

    Markwardt, Niklas; Götz, Marcus; Haj-Hosseini, Neda; Hollnburger, Bastian; Sroka, Ronald; Stepp, Herbert; Zelenkov, Petr; Rühm, Adrian

    2016-04-01

    5-aminolevulinic-acid-(5-ALA)-induced protoporphyrin IX (PpIX) fluorescence may be used to improve stereotactic brain tumor biopsies. In this study, the sensitivity of PpIX-based tumor detection has been investigated for two potential excitation wavelengths (405 nm, 633 nm). Using a 200 μm fiber in contact with semi-infinite optical phantoms containing ink and Lipovenös, PpIX detection limits of 4.0 nM and 200 nM (relating to 1 mW excitation power) were determined for 405 nm and 633 nm excitation, respectively. Hence, typical PpIX concentrations in glioblastomas of a few μM should be well detectable with both wavelengths. Additionally, blood layers of selected thicknesses were placed between fiber and phantom. Red excitation was shown to be considerably less affected by blood interference: A 50 μm blood layer, for instance, blocked the 405- nm-excited fluorescence completely, but reduced the 633-nm-excited signal by less than 50%. Ray tracing simulations demonstrated that - without blood layer - the sensitivity advantage of 405 nm rises for decreasing fluorescent volume from 50-fold to a maximum of 100-fold. However, at a tumor volume of 1 mm3, which is a typical biopsy sample size, the 633-nm-excited fluorescence signal is only reduced by about 10%. Further simulations revealed that with increasing fiber-tumor distance, the signal drops faster for 405 nm. This reduces the risk of detecting tumor tissue outside the needle's coverage, but diminishes the overlap between optically and mechanically sampled volumes. While 405 nm generally offers a higher sensitivity, 633 nm is more sensitive to distant tumors and considerably superior in case of blood-covered tumor tissue.

  12. Investigations of a Dual Seeded 1178 nm Raman Laser System

    Science.gov (United States)

    2016-01-14

    constructed using slab1-3 or fiber laser technology4-15. Slab technology generally involves sum-frequency mixing of 1064 and 1319 nm in a lithium...triborate crystal to obtain 589 nm. Another way of achieving the desired output wavelength of 589 nm for sodium guidestar laser applications is through...been obtained from an ytterbium-doped photonic band gap fiber laser with a 320 kHz linewidth13. Finally, 85 W of single frequency (1 MHz) 1178 nm was

  13. 7nm logic optical lithography with OPC-Lite

    Science.gov (United States)

    Smayling, Michael C.; Tsujita, Koichiro; Yaegashi, Hidetami; Axelrad, Valery; Nakayama, Ryo; Oyama, Kenichi; Yamauchi, Shohei; Ishii, Hiroyuki; Mikami, Koji

    2015-03-01

    The CMOS logic 22nm node was the last one done with single patterning. It used a highly regular layout style with Gridded Design Rules (GDR). Smaller nodes have required the same regular layout style but with multiple patterning for critical layers. A "line/cut" approach is being used to achieve good pattern fidelity and process margin.[1] As shown in Fig. 1, even with "line" patterns, pitch division will eventually be necessary. For the "cut" pattern, Design-Source-Mask Optimization (DSMO) has been demonstrated to be effective at the 20nm node and below.[2,3,4] Single patterning was found to be suitable down to 16nm, while double patterning extended optical lithography for cuts to the 10-12nm nodes. Design optimization avoided the need for triple patterning. Lines can be patterned with 193nm immersion with no complex OPC. The final line dimensions can be achieved by applying pitch division by two or four.[5] In this study, we extend the scaling using simplified OPC to the 7nm node for critical FEOL and BEOL layers. The test block is a reasonably complex logic function with ~100k gates of combinatorial logic and flip-flops, scaled from previous experiments. Simulation results show that for cuts at 7nm logic dimensions, the gate layer can be done with single patterning whose minimum pitch is 53nm, possibly some of the 1x metal layers can be done with double patterning whose minimum pitch is 53nm, and the contact layer will require triple patterning whose minimum pitch is 68nm. These pitches are less than the resolution limit of ArF NA=1.35 (72nm). However these patterns can be separated by a combination of innovative SMO for less than optical resolution limit and a process trick of hole-repair technique. An example of triple patterning coloring is shown in Fig 3. Fin and local interconnect are created by lines and trims. The number of trim patterns are 3 times (min. pitch=90nm) and twice (min. pitch=120nm), respectively. The small number of masks, large pitches, and

  14. First principles study on the interfacial properties of NM/graphdiyne (NM = Pd, Pt, Rh and Ir): The implications for NM growing

    Energy Technology Data Exchange (ETDEWEB)

    Lu, Zhansheng; Li, Shuo; Lv, Peng [College of Physics and Electronic Engineering, Henan Normal University, Xinxiang 453007 (China); He, Chaozheng, E-mail: hecz2013@nynu.edu.cn [College of Physics and Electronic Engineering, Nanyang Normal University, Nanyang 473061 (China); Ma, Dongwei [School of Physics, Anyang Normal University, Anyang 455000 (China); Yang, Zongxian, E-mail: yzx@henannu.edu.cn [College of Physics and Electronic Engineering, Henan Normal University, Xinxiang 453007 (China); Collaborative Innovation Center of Nano Functional Materials and Applications, Kaifeng (China)

    2016-01-01

    Graphical abstract: - Highlights: • The NM adatoms belong to embedded adsorption in 18C-hexagon of GDY. • The Rh and Ir/GDY can be applied to single metal catalysts or sensors. • A simple linear relationship between E{sub e-ads} and E{sub b} is presented. • The linear relationship can be used in the noble metal modified GDY. - Abstract: Based on the dispersion-corrected density functional calculations (DFT-D), we systematically studied the adsorption of noble metals (NM), Pd, Pt, Rh and Ir, on graphdiyne (GDY). We present a systematic study on the geometry, embedded adsorption energy and electronic structure of four different adatoms adsorbed on the GDY. The strong interaction between the NM adatoms and the GDY substrate is found with the NM embedded in the 18C-hexagon of the GDY. We investigated the mobility of the NM adatoms on the GDY, and found that the mobility barrier energy increases along with the increasing of the embedded adsorption energy. We present the NM adatoms growth of high concentrations on the GDY. Upon the analysis of the electronic structure and the frontier molecular orbitals, Rh and Ir adatoms of low concentrations (about 1.37 at%) on the GDY have the potential to be applied as single metal catalysts or gas molecule sensors.

  15. A 1.5-W frequency doubled semiconductor disk laser tunable over 40 nm at around 745 nm

    Science.gov (United States)

    Saarinen, Esa J.; Lyytikäinen, Jari; Ranta, Sanna; Rantamäki, Antti; Saarela, Antti; Sirbu, Alexei; Iakovlev, Vladimir; Kapon, Eli; Okhotnikov, Oleg G.

    2016-03-01

    We report on a semiconductor disk laser emitting 1.5 W of output power at the wavelength of 745 nm via intracavity frequency doubling. The high power level and the pumped with commercial low-cost 980 nm laser diode modules. Laser emission at 1490 nm was frequency-doubled with a bismuth borate crystal that was cut for type I critical phase matching. At the maximum output power, we achieved an optical-to-optical efficiency of 8.3% with beam quality parameter M2 below 1.5. The laser wavelength could be tuned with an intracavity birefringent plate from 720 to 764 nm.

  16. Evaluation of dental pulp repair using low level laser therapy (688 nm and 785 nm) morphologic study in capuchin monkeys

    Science.gov (United States)

    Pretel, H.; Oliveira, J. A.; Lizarelli, R. F. Z.; Ramalho, L. T. O.

    2009-02-01

    The aim of this study was to evaluate the hypothesis that low-level laser therapy (LLLT) 688 nm and 785 nm accelerate dentin barrier formation and repair process after traumatic pulp exposure. The sample consisted of 45 premolars of capuchin monkeys (Cebus apella) with pulp exposure Class V cavities. All premolars were treated with calcium hydroxide (Ca(OH)2), divided in groups of 15 teeth each, and analyzed on 7th, 25th, and 60th day. Group GI - only Ca(OH)2, GII - laser 688 nm, and GIII - laser 785 nm. Laser beam was used in single and punctual dose with the parameters: continuous, 688 nm and 785 nm wavelength, tip's area of 0.00785 cm2, power 50 mW, application time 20 s, dose 255 J/cm2, energy 2 J. Teeth were capped with Ca(OH)2, Ca(OH)2 cement and restored with amalgam. All groups presented pulp repair. On 25th day the thickness of the formed dentin barrier was different between the groups GI and GII (p < 0.05) and between groups GI and GIII (p < 0.01). On 60th day there was difference between GI and GIII (p < 0.01). It may be concluded that, LLLT 688 nm and 785 nm accelerated dentin barrier formation and consequently pulp repair process, with best results using infrared laser 785 nm.

  17. On high speed transmission with the 850nm VCSELs

    Science.gov (United States)

    Turkiewicz, Jarosław P.; Chorchos, Łukasz; Puerta Ramirez, Rafael; Vegas Olmos, Juan Jose; Ledentsov, Nikolay

    2016-09-01

    One of the key research challenges is development of energy efficient high bit rate data interconnects. The most promising solutions are based on 850 nm vertical cavity surface emitting lasers (VCSEL) and multi mode fibre (MMF). In this paper options to realize energy efficient 850 nm data interconnects are discussed and evaluated.

  18. On high speed transmission with the 850 nm VCSELs

    DEFF Research Database (Denmark)

    Turkiewicz, Jarosław P.; Chorchos, Łukasz; Puerta Ramírez, Rafael

    2016-01-01

    One of the key research challenges is development of energy efficient high bit rate data interconnects. The most promising solutions are based on 850 nm vertical cavity surface emitting lasers (VCSEL) and multi mode fibre (MMF). In this paper options to realize energy efficient 850 nm data...

  19. A photonic crystal fiber with zero dispersion at 1064 nm

    DEFF Research Database (Denmark)

    Andersen, Peter Andreas

    2002-01-01

    We report on the dispersion properties of a single mode, large core photonic crystal fiber. Using white light interferometry the fiber is found to have zero dispersion at 1064 nm......We report on the dispersion properties of a single mode, large core photonic crystal fiber. Using white light interferometry the fiber is found to have zero dispersion at 1064 nm...

  20. A New Solar Spectrum from 656 to 3088 nm

    Science.gov (United States)

    Meftah, M.; Damé, L.; Bolsée, D.; Pereira, N.; Sluse, D.; Cessateur, G.; Irbah, A.; Sarkissian, A.; Djafer, D.; Hauchecorne, A.; Bekki, S.

    2017-08-01

    The solar spectrum is a key parameter for different scientific disciplines such as solar physics, climate research, and atmospheric physics. The SOLar SPECtrometer (SOLSPEC) instrument of the Solar Monitoring Observatory (SOLAR) payload onboard the International Space Station (ISS) has been built to measure the solar spectral irradiance (SSI) from 165 to 3088 nm with high accuracy. To cover the full wavelength range, three double-monochromators with concave gratings are used. We present here a thorough analysis of the data from the third channel/double-monochromator, which covers the spectral range between 656 and 3088 nm. A new reference solar spectrum is therefore obtained in this mainly infrared wavelength range (656 to 3088 nm); it uses an absolute preflight calibration performed with the blackbody of the Physikalisch-Technische Bundesanstalt (PTB). An improved correction of temperature effects is also applied to the measurements using in-flight housekeeping temperature data of the instrument. The new solar spectrum (SOLAR-IR) is in good agreement with the ATmospheric Laboratory for Applications and Science (ATLAS 3) reference solar spectrum from 656 nm to about 1600 nm. However, above 1600 nm, it agrees better with solar reconstruction models than with spacecraft measurements. The new SOLAR/SOLSPEC measurement of solar spectral irradiance at about 1600 nm, corresponding to the minimum opacity of the solar photosphere, is 248.08 ± 4.98 mW m-2 nm-1 (1 σ), which is higher than recent ground-based evaluations.

  1. Development and application of UV excimer lamps from 354nm -126nm

    Science.gov (United States)

    Boyd, Ian W.; Liaw, Irving I.

    2006-05-01

    The use of high intensity ultraviolet (UV) and vacuum ultraviolet (VUV) radiation generated from decaying excimer complexes through dielectric barrier discharge (silent discharges) sources for the purposes of surface processing and modification is reviewed. Such sources provide a singular dominant narrow-band emission at various wavelengths(λ) between 126 - 354 nm. The remarkable simplicity of supplying these sources and flexibility of their geometric configurations allow them to be coupled in parallel thus providing high photon fluxes over large areas. The monochromatic selectivity allows for application to process and chemical pathway specific tasks by simple variation of the discharge gas mixture. These sources are an interesting addition to and as an alternative to lasers for large scale industrial applications and their unique characterisitics have led to their use in a number of low-temperature material modification techniques, some of which are reviewed here. These include the photo-induced low-temperature formation of oxynitride layers, high-κ thin film layers and the post-deposition annealing of pulsed laser deposited (PLD) thin films.

  2. Quantitative analysis of ChIP-seq data uncovers dynamic and sustained H3K4me3 and H3K27me3 modulation in cancer cells under hypoxia.

    Science.gov (United States)

    Adriaens, Michiel E; Prickaerts, Peggy; Chan-Seng-Yue, Michelle; van den Beucken, Twan; Dahlmans, Vivian E H; Eijssen, Lars M; Beck, Timothy; Wouters, Bradly G; Voncken, Jan Willem; Evelo, Chris T A

    2016-01-01

    A comprehensive assessment of the epigenetic dynamics in cancer cells is the key to understanding the molecular mechanisms underlying cancer and to improving cancer diagnostics, prognostics and treatment. By combining genome-wide ChIP-seq epigenomics and microarray transcriptomics, we studied the effects of oxygen deprivation and subsequent reoxygenation on histone 3 trimethylation of lysine 4 (H3K4me3) and lysine 27 (H3K27me3) in a breast cancer cell line, serving as a model for abnormal oxygenation in solid tumors. A priori, epigenetic markings and gene expression levels not only are expected to vary greatly between hypoxic and normoxic conditions, but also display a large degree of heterogeneity across the cell population. Where traditionally ChIP-seq data are often treated as dichotomous data, the model and experiment here necessitate a quantitative, data-driven analysis of both datasets. We first identified genomic regions with sustained epigenetic markings, which provided a sample-specific reference enabling quantitative ChIP-seq data analysis. Sustained H3K27me3 marking was located around centromeres and intergenic regions, while sustained H3K4me3 marking is associated with genes involved in RNA binding, translation and protein transport and localization. Dynamic marking with both H3K4me3 and H3K27me3 (hypoxia-induced bivalency) was found in CpG-rich regions at loci encoding factors that control developmental processes, congruent with observations in embryonic stem cells. In silico-identified epigenetically sustained and dynamic genomic regions were confirmed through ChIP-PCR in vitro, and obtained results are corroborated by published data and current insights regarding epigenetic regulation.

  3. Se14, encoding a JmjC domain-containing protein, plays key roles in long-day suppression of rice flowering through the demethylation of H3K4me3 of RFT1.

    Directory of Open Access Journals (Sweden)

    Takayuki Yokoo

    Full Text Available Floral transition from the vegetative to the reproductive growth phase is a major change in the plant life cycle and a key factor in reproductive success. In rice (Oryza sativa L., a facultative short-day plant, numerous flowering time and flower formation genes that control floral transition have been identified and their physiological effects and biochemical functions have been clarified. In the present study, we used a Se14-deficient mutant line (HS112 and other flowering mutant lines to investigate the photoperiodic response, chromosomal location and function in the photoperiod sensitivity of the Se14 gene. We also studied the interactive effects of this locus with other crucial flowering time genes. We found that Se14 is independent of the known photoperiod-sensitive genes, such as Hd1 and Ghd7, and is identical to Os03g0151300, which encodes a Jumonji C (JmjC domain-containing protein. Expression analysis revealed that the expressions of RFT1, a floral initiator known as a "florigen-like gene", and Ehd1 were up-regulated in HS112, whereas this up-regulation was not observed in the original variety of 'Gimbozu'. ChIP assays of the methylation states of histone H3 at lysine 4 (H3K4 revealed that the trimethylated H3K4 in the promoter region of the RFT1 chromatin was significantly increased in HS112. We conclude that Se14 is a novel photoperiod-sensitivity gene that has a suppressive effect on floral transition (flowering time under long day-length conditions through the modification of chromatin structure by H3K4me3 demethylation in the promoter region of RFT1.

  4. Applications of the 308-nm excimer laser in dermatology

    Science.gov (United States)

    Farkas, A.; Kemeny, L.

    2006-05-01

    Excimer lasers contain a mixture of a noble inert gas and a halogen, which form excited dimers only in the activated state. High-energy current is used to produce these dimers, which have a very short lifetime, and after their fast dissociation they release the excitation energy through ultraviolet photons. The application of these lasers proved to be successful in medicine, including the field of ophthalmology, cardiology, angiology, dentistry, orthopaedics, and, in recent years, dermatology. For medical purposes, the 193-nm argon fluoride, the 248-nm krypton fluoride, the 351-nm xenon fluoride, and the 308-nm xenon chloride lasers are used. Recently, the 308-nm xenon chloride laser has gained much attention as a very effective treatment modality in dermatological disorders. It was successfully utilized in psoriasis; later, it proved to be useful in handling other lightsensitive skin disorders and even in the treatment of allergic rhinitis. This review summarizes the possible applications of this promising tool in dermatology.

  5. Picosecond laser texturization of mc-silicon for photovoltaics: A comparison between 1064 nm, 532 nm and 355 nm radiation wavelengths

    Energy Technology Data Exchange (ETDEWEB)

    Binetti, Simona [Department of Materials Science and Milano-Bicocca Solar Energy Research Center (MIB-SOLAR), University of Milano-Bicocca, Via Cozzi 55, 20125 Milano (Italy); Le Donne, Alessia, E-mail: alessia.ledonne@mater.unimib.it [Department of Materials Science and Milano-Bicocca Solar Energy Research Center (MIB-SOLAR), University of Milano-Bicocca, Via Cozzi 55, 20125 Milano (Italy); Rolfi, Andrea [Department of Materials Science and Milano-Bicocca Solar Energy Research Center (MIB-SOLAR), University of Milano-Bicocca, Via Cozzi 55, 20125 Milano (Italy); Jäggi, Beat; Neuenschwander, Beat [Bern University of Applied Sciences, Engineering and Information Technology, Institute for Applied Laser, Photonics and Surface Technologies ALPS, Pestalozzistrasse 20, CH-3400 Burgdorf (Switzerland); Busto, Chiara [ENI Spa, Via Giacomo Fauser, 4, 28100 Novara (Italy); Frigeri, Cesare [CNR-IMEM Institute, Parco Area Delle Scienze 37/A, Fontanini, 43010 Parma (Italy); Scorticati, Davide; Longoni, Luca; Pellegrino, Sergio [Laserpoint Srl, Via Della Burrona 51, 20090 Vimodrone, Milano (Italy)

    2016-05-15

    Highlights: • Self-organized surface structures were produced by picosecond laser pulses on mc-Si. • Three laser wavelengths were used which effectively reduce Si reflectivity up to 8%. • The subsurface damage induced by the three lasers was studied in detail. • μ-Raman, PL and TEM proved that UV laser provides the lowest subsurface damage. • UV laser induced damage is located above the depletion region of the p–n junction. - Abstract: Self-organized surface structures were produced by picosecond laser pulses on multi-crystalline silicon for photovoltaic applications. Three different laser wavelengths were employed (i.e. 1064 nm, 532 nm and 355 nm) and the resulting morphologies were observed to effectively reduce the reflectivity of the samples after laser irradiation. Besides, a comparative study of the laser induced subsurface damage generated by the three different wavelengths was performed by confocal micro-Raman, photoluminescence and transmission electron microscopy. The results of both the structural and optical characterization showed that the mc-Si texturing performed with the laser at 355 nm provides surface reflectivity between 11% and 8% over the spectral range from 400 nm to 1 μm, while inducing the lowest subsurface damage, located above the depletion region of the p–n junction.

  6. VUV radiometry below 100 nm: the high-power hydrogen arc as a standard source of continuum radiation between 53 nm and 92 nm.

    Science.gov (United States)

    Behringer, K; Thoma, P

    1979-08-01

    A stationary hydrogen arc discharge may be used as a standard source of radiation in the VUV below 100 nm. The Lyman resonance continuum is used, the cross sections of which are theoretically well known. The method described is based on optically thin radiation, requiring high plasma temperatures and an effective helium gas separation in the arc. The investigations demonstrate that, in appropriate experimental conditions, the plasma is indeed transparent down to the onset of the He ground-state absorption. Above that, the VUV spectral radiance can be predicted within less than 15% uncertainty from conventional plasma diagnostics. For a first application, the He continuum has been measured between 65 nm and 92 nm. The consistency of these results with theoretical calculations confirms the validity of the concept presented.

  7. Development of 2-channel (532 nm and 355 nm) mobile LIDAR for mapping particulate matter in the atmosphere

    CSIR Research Space (South Africa)

    Sivakumar, V

    2010-09-01

    Full Text Available In this paper, the authors describe the developmentof 2-Channel (532 nm and 355 nm) mobile LIDAR system for studying atmospheric particulate matter. The system is currently tested in house at the Council for Scientific and Industrial Research...

  8. On the photochemistry of IONO2: absorption cross section (240-370 nm) and photolysis product yields at 248 nm.

    Science.gov (United States)

    Joseph, D M; Ashworth, S H; Plane, J M C

    2007-11-01

    The absolute absorption cross section of IONO(2) was measured by the pulsed photolysis at 193 nm of a NO(2)/CF(3)I mixture, followed by time-resolved Fourier transform spectroscopy in the near-UV. The resulting cross section at a temperature of 296 K over the wavelength range from 240 to 370 nm is given by log(10)(sigma(IONO(2))/cm(2) molecule(-1)) = 170.4 - 3.773 lambda + 2.965 x 10(-2)lambda(2)- 1.139 x 10(-4)lambda(3) + 2.144 x 10(-7)lambda(4)- 1.587 x 10(-10)lambda(5), where lambda is in nm; the cross section, with 2sigma uncertainty, ranges from (6.5 +/- 1.9) x 10(-18) cm(2) at 240 nm to (5 +/- 3) x 10(-19) cm(2) at 350 nm, and is significantly lower than a previous measurement [J. C. Mössinger, D. M. Rowley and R. A. Cox, Atmos. Chem. Phys., 2002, 2, 227]. The photolysis quantum yields for IO and NO(3) production at 248 nm were measured using laser induced fluorescence of IO at 445 nm, and cavity ring-down spectroscopy of NO(3) at 662 nm, yielding phi(IO) iodine oxides, but the formation and subsequent photolysis of IONO(2) is very inefficient as an ozone-depleting cycle.

  9. Mighty high-T lithography for 65-nm generation contacts

    Science.gov (United States)

    Conley, Will; Montgomery, Patrick K.; Lucas, Kevin; Litt, Lloyd C.; Maltabes, John G.; Dieu, Laurent; Hughes, Gregory P.; Mellenthin, David L.; Socha, Robert J.; Fanucchi, Eric L.; Verhappen, Arjan; Wampler, Kurt E.; Yu, Linda; Schaefer, Erika; Cassel, Shawn; Kuijten, Jan P.; Pijnenburg, Wil; Wiaux, Vincent; Vandenberghe, Geert

    2003-06-01

    Contact patterning for the 65nm device generation will be an exceedingly difficult task. The 2001 SIA roadmap lists the targeted contact size as 90nm with +/-10% CD control requirements of +/-9nm. Defectivity levels must also be below one failure per billion contacts for acceptable device yield. Difficulties in contact patterning are driven by the low depth of focus of isolated contacts and/or the high mask error (MEF) for dense contact arrays (in combination with expected reticle CD errors). Traditional contact lithography methods are not able to mitigate both these difficulties simultaneously. Inlaid metal trench patterning for the 65nm generation has similar lithographic difficulties though not to the extreme degree as seen with contacts. This study included the use of multiple, high transmission, 193nm attenuated phase shifting mask varieties to meet the difficult challenges of 65nm contact and trench lithography. Numerous illumination schemes, mask biasing, optical proximity correction (OPC), mask manufacturing techniques, and mask blank substrate materials were investigated. The analysis criteria included depth of focus, exposure latitude and MEF through pitch, reticle inspection, reticle manufacturability, and cost of ownership. The investigation determined that certain high transmission reticle schemes are strong contenders for 65nm generation contact and trench patterning. However, a number of strong interactions between illumination, OPC, and reticle manufacturing issues need to be considered.

  10. Kinetics and polymorphs of yeast prion Sup35NM amyloidogenesis.

    Science.gov (United States)

    Kinoshita, Misaki; Lin, Yuxi; Nakatsuji, Masatoshi; Inui, Takashi; Lee, Young-Ho

    2017-09-01

    Amyloidogenic proteins often form many types of aggregates, which are a critical determinant of cytotoxicity and tissue specificity. However, the molecular mechanisms underlying the generation of distinct amyloids and their influence on cells remain largely unknown. We herein investigated the polymorphic amyloid formation of the yeast prion protein, Sup35NM, an intrinsically disordered N-terminal fragment of Sup35, under various conditions and its potential relationship to cytotoxicity. Sup35NM aggregated to amyloid fibrils with distinct kinetics, structures, morphologies, tinctorial properties, and conformational stabilities depending on the concentration of NaCl, pH, and temperature, indicating the polymorphic amyloidogenesis of Sup35NM. Detailed kinetic analyses of Sup35NM amyloid formation revealed a strong inverse correlation between the lag time and elongation rate without a correlation between kinetic and structural parameters. These results suggest that kinetic polymorphisms due to distinct nucleation and elongation rates result in structural polymorphs of amyloid fibrils, and also that conditions that enhance or inhibit the nucleation of Sup35NM promote or delay fibril growth. The deleterious effects of polymorphic Sup35NM amyloid fibrils on membrane integrity and cell vitality were minimal. We hypothesize that the innocuous polymorphic nature of Sup35NM amyloid fibrils may be beneficial for gaining time for prion infection prior to cell death. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. EUV reticle inspection with a 193nm reticle inspector

    Science.gov (United States)

    Broadbent, William; Inderhees, Gregg; Yamamoto, Tetsuya; Lee, Isaac; Lim, Phillip

    2013-06-01

    The prevailing industry opinion is that EUV Lithography (EUVL) will enter High Volume Manufacturing (HVM) in the 2015 - 2017 timeframe at the 16nm HP node. Every year the industry assesses the key risk factors for introducing EUVL into HVM - blank and reticle defects are among the top items. To reduce EUV blank and reticle defect levels, high sensitivity inspection is needed. To address this EUV inspection need, KLA-Tencor first developed EUV blank inspection and EUV reticle inspection capability for their 193nm wavelength reticle inspection system - the Teron 610 Series (2010). This system has become the industry standard for 22nm / 3xhp optical reticle HVM along with 14nm / 2xhp optical pilot production; it is further widely used for EUV blank and reticle inspection in R and D. To prepare for the upcoming 10nm / 1xhp generation, KLA-Tencor has developed the Teron 630 Series reticle inspection system which includes many technical advances; these advances can be applied to both EUV and optical reticles. The advanced capabilities are described in this paper with application to EUV die-to-database and die-to-die inspection for currently available 14nm / 2xhp generation EUV reticles. As 10nm / 1xhp generation optical and EUV reticles become available later in 2013, the system will be tested to identify areas for further improvement with the goal to be ready for pilot lines in early 2015.

  12. Fluorescence Imaging In Vivo up to 1700 nm

    CERN Document Server

    Diao, Shuo; Hong, Guosong; Antaris, Alexander L; Chang, Junlei; Wu, Justin Z; Zhang, Bo; Kuo, Calvin J; Dai, Hongjie

    2015-01-01

    Compared to visible and near-infrared regions below ~ 900 nm, imaging in the second near-infrared window beyond 1000 nm (NIR-II, 1000-1700 nm) is promising for deep-tissue high-resolution optical imaging in vivo owing to reduced scattering of photons traversing through tissues. Here, we succeeded fluorescence imaging in vivo in the long 1500-1700 nm (NIR-IIb) region using a novel, chemical separation enriched large-diameter semiconducting single-walled carbon nanotube material. Imaging in the 1500-1700 nm window resolved 3-4 um wide capillary blood vessels at ~ 3 millimeters depth through the intact body and brain of mice with the ability of blood-flow speed mapping in individual capillary vessels. Further, non-invasive single fluorophore imaging inside the tumor of a live mouse was achieved in the 1500-1700 nm window. NIR-IIb imaging can be generalized to a wide range of fluorophores emitting up to 1700 nm for a new paradigm of high performance in vivo optical imaging.

  13. Inexpensive Home-Made Single Wavelength Ellipsometer (λ = 633 nm) for Measuring the Optical Constant of Nanostructured Materials

    Science.gov (United States)

    Maulana, L. Z.; Megasari, K.; Suharyadi, E.; Anugraha, R.; Abraha, K.; Santoso, I.

    2017-05-01

    Inexpensive home-made Single wavelength Ellipsometry with RAE (Rotating Analyser Ellipsometer) configuration has been developed. Spectroscopic ellipsometry (SE) is an optical measurement technique which is based on the measurement of the change of the phase difference (Δ) and the amplitude ratio (ψ) between p and s linear polarized of reflected (or transmitted) light. Our RAE configuration system composed of polarizer, sample, analyzer, detector, and He-Ne laser (λ = 633 nm) that acted as the monochromatic light source. To test the reliability of our SE system, we measure the optical constant of Au bulk and Cr (30 nm thick) film. The optical constant and the thickness were extracted by employing the pseudo-dielectric function and numerical inversion which is based on the secant method, the ψ and Δ of our SE data which is modelled by Fresnel equation. From the extraction using the secant method we obtain the optical constant of the Au bulk sample with n = 0.11 to 0.22 and k = 3.26 to 3.37 which is close to that of using pseudo-dielectric method. We obtain the same result for Cr film with n = 3.66 to 3.81 and k = 5.32 to 5.38 which is close to the result from reference. These results show that our inexpensive home-made Single wavelength Ellipsometry instrument and the extraction method are reliable for determining the optical constant of nanostructured materials.

  14. Enhanced photoelectrocatalytic decomposition of copper cyanide complexes and simultaneous recovery of copper with a Bi2MoO6 electrode under visible light by EDTA/K4P2O7.

    Science.gov (United States)

    Zhao, Xu; Zhang, Juanjuan; Qiao, Meng; Liu, Huijuan; Qu, Jiuhui

    2015-04-07

    Simultaneous photoelectrocatalytic (PEC) oxidation of cyanides and recovery of copper in a PEC reactor with a Bi(2)MoO(6) photoanode was investigated at alkaline conditions under visible light irradiation. The surface variation of the Bi(2)MoO(6) photoanode and titanium cathode was characterized. The Cu mass distribution onto the anode, in the solution, and onto the cathode was fully investigated. In the individual PEC oxidation of copper cyanides, the formation of a black copper oxide on the anode occurred. By keeping the initial cyanide concentration at 0.01 mM, the effect of EDTA/K(4)P(2)O(7) was examined at different molar ratios of EDTA/K(4)P(2)O(7) to cyanide. It was indicated that the oxidation of cyanides increased and simultaneous copper electrodeposition with zero value onto the cathode was feasible at pH 11. Under the optimal conditions, the total cyanide concentration was lowered from 250 to 5.0 mg/L, and the Cu recovery efficiency deposited onto the cathode was higher than 90%. Cyanate was the only product. The role of the photogenerated hole in the oxidation of cyanide ions was confirmed.

  15. 80 nm tunable DBR-free semiconductor disk laser

    Science.gov (United States)

    Yang, Z.; Albrecht, A. R.; Cederberg, J. G.; Sheik-Bahae, M.

    2016-07-01

    We report a widely tunable optically pumped distributed Bragg reflector (DBR)-free semiconductor disk laser with 6 W continuous wave output power near 1055 nm when using a 2% output coupler. Using only high reflecting mirrors, the lasing wavelength is centered at 1034 nm and can be tuned up to a record 80 nm by using a birefringent filter. We attribute such wide tunability to the unique broad effective gain bandwidth of DBR-free semiconductor disk lasers achieved by eliminating the active mirror geometry.

  16. Tapered diode laser pumped 946 nm Nd:YAG laser

    DEFF Research Database (Denmark)

    Cheng, Haynes Pak Hay; Jensen, Ole Bjarlin; Petersen, Paul Michael

    2009-01-01

    We successfully implemented a 946 nm Nd:YAG laser based on a 808 nm tapered diode pump laser. The tapered diode is developed at the Ferdinand-Braun-Institute fur Hochstfrequenztechnik in Germany. Figure 2 shows the experimental setup and results of each pump source coupled into a 1.5 mm crystal...... laser, we show that tapered diode laser pumping potentially increase the power of 946 nm lasers by a factor of two and reduce the threshold by a factor of three....

  17. K4W2OCl10

    Science.gov (United States)

    Villars, P.; Cenzual, K.; Daams, J.; Gladyshevskii, R.; Shcherban, O.; Dubenskyy, V.; Kuprysyuk, V.; Savysyuk, I.; Zaremba, R.

    This document is part of Subvolume A10 'Structure Types. Part 10: Space Groups (140) I4/mcm - (136) P42/mnm' of Volume 43 'Crystal Structures of Inorganic Compounds' of Landolt-Börnstein - Group III 'Condensed Matter'.

  18. Efficient laser operation of Nd3+:Lu2O3 at various wavelengths between 917 nm and 1463 nm

    Science.gov (United States)

    von Brunn, P.; Heuer, A. M.; Fornasiero, L.; Huber, G.; Kränkel, C.

    2016-08-01

    Even though the first Nd3+-doped sesquioxide lasers have been realized more than 50 years ago, up to now no reports on efficient laser operation of Nd3+:doped sesquioxides can be found. In this work, we review the favorable spectroscopic properties of the sesquioxide Nd3+:Lu2O3 in terms of ground state absorption, stimulated emission, and excited state absorption cross sections as well as the upper level lifetime. Making use of these properties, we achieved efficient laser performance on eight different laser transitions in the wavelength range between 917 nm and 1463 nm under Ti:sapphire laser pumping using state-of-the-art HEM-grown Nd3+:Lu2O3 crystals with good optical quality. At the strongest transition around 1076 nm we determined a slope efficiency of 69%, which represents the highest efficiency ever obtained for a Nd3+-doped sesquioxide. Furthermore, we could generate watt level output powers and high slope efficiencies for seven other transitions. Lasers at 917 nm, 1053 nm, 1108 nm and 1463 nm were realized for the first time and the latter represents one of the longest laser wavelengths obtained on the 4F3/2  →  4I13/2 transition in Nd3+-doped materials.

  19. Compact 2050 nm Semiconductor Diode Laser Master Oscillator Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This Phase I effort seeks to develop DFB laser master oscillators at the novel wavelength of 12050 nm. Two prototypes will be built, tested, and delivered ....

  20. EST Table: NM_001177325 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001177325 rcd1 10/09/29 100 %/294 aa ref|NP_001170796.1| cell differentiation protein RCD...15.1| PREDICTED: similar to cell differentiation protein rcd1 [Tribolium castaneum] FS909406 ...

  1. RadNet Air Data From Navajo Lake, NM

    Science.gov (United States)

    This page presents radiation air monitoring and air filter analysis data for Navajo Lake, NM from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  2. Space-Qualified 1064 nm Seed and Metrology Laser Project

    Data.gov (United States)

    National Aeronautics and Space Administration — A space-qualified, single-frequency oscillator operating at 1064 nm is a critical component for a number of active optical measurement systems that have been...

  3. 1300-nm gain obtained with dysprosium-doped chloride crystals

    Energy Technology Data Exchange (ETDEWEB)

    Page, R.H.; Schaffers, K.I.; Beach, R.J.; Payne, S.A.; Krupke, W.F.

    1996-03-01

    Dy{sup 3+} - doped chloride crystals have high 1300-nm emission quantum yields. Pump - probe experiments on La Cl{sub 3}:Dy{sup 3+} demonstrate optical gain consistent with predictions based on spectroscopic cross sections and lifetimes.

  4. EST Table: NM_001126235 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001126235 Tra2 10/09/29 76 %/156 aa ref|NP_001119705.1| transformer 2 isoform A ...[Bombyx mori] gb|AAT42220.2| transformer-2 protein A [Bombyx mori] 10/09/13 65 %/101 aa FBpp0236157|Dvira2-P...gi|189240798|ref|XP_968550.2| PREDICTED: similar to transformer-2 protein A [Tribolium castaneum] NM_001126237 ...

  5. EST Table: NM_001126236 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001126236 Tra2 10/09/29 57 %/289 aa ref|NP_001119708.1| transformer 2 isoform D ...[Bombyx mori] gb|AAX47002.1| transformer-2 protein D [Bombyx mori] 10/09/13 65 %/101 aa FBpp0236157|Dvira2-P...gi|189240798|ref|XP_968550.2| PREDICTED: similar to transformer-2 protein A [Tribolium castaneum] NM_001126237 ...

  6. EST Table: NM_001126234 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001126234 Tra2 10/09/29 76 %/156 aa ref|NP_001119705.1| transformer 2 isoform A ...[Bombyx mori] gb|AAT42220.2| transformer-2 protein A [Bombyx mori] 10/09/13 65 %/101 aa FBpp0236157|Dvira2-P...gi|189240798|ref|XP_968550.2| PREDICTED: similar to transformer-2 protein A [Tribolium castaneum] NM_001126237 ...

  7. EST Table: NM_001126233 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001126233 Tra2 10/09/29 60 %/266 aa ref|NP_001119705.1| transformer 2 isoform A ...[Bombyx mori] gb|AAT42220.2| transformer-2 protein A [Bombyx mori] 10/09/13 65 %/101 aa FBpp0236157|Dvira2-P...gi|189240798|ref|XP_968550.2| PREDICTED: similar to transformer-2 protein A [Tribolium castaneum] NM_001126237 ...

  8. Highly Stable PM Raman Fiber Laser at 1680 nm

    DEFF Research Database (Denmark)

    Svane, Ask Sebastian; Liu, Xiaomin; Rottwitt, Karsten

    2013-01-01

    We demonstrate thermal stabilization of a Raman fiber laser. At 1680 nm the laser emission exceeds 500 mW with a power variation below 0.5 %, both linewidth and wavelength variations are under 1 pm.......We demonstrate thermal stabilization of a Raman fiber laser. At 1680 nm the laser emission exceeds 500 mW with a power variation below 0.5 %, both linewidth and wavelength variations are under 1 pm....

  9. Cascaded Soliton Compression of Energetic Femtosecond Pulses at 1030 nm

    DEFF Research Database (Denmark)

    Bache, Morten; Zhou, Binbin

    2012-01-01

    We discuss soliton compression with cascaded second-harmonic generation of energetic femtosecond pulses at 1030 nm. We discuss problems encountered with soliton compression of long pulses and show that sub-10 fs compressed pulses can be achieved.......We discuss soliton compression with cascaded second-harmonic generation of energetic femtosecond pulses at 1030 nm. We discuss problems encountered with soliton compression of long pulses and show that sub-10 fs compressed pulses can be achieved....

  10. Diode-pumped 1123-nm Nd:YAG laser

    Institute of Scientific and Technical Information of China (English)

    Xiaoping Guo(郭晓萍); Meng Chen(陈檬); Gang Li(李港); Bingyuan zhang(张炳元); Jiandong Yang(杨建东); Zhigang Zhang(张志刚); Yonggang Wang(王勇刚)

    2004-01-01

    We demonstrated a diode-pumped Nd:YAG laser with a plano-concave resonator. When the pump power is 1.57 W, the output power of 1123-nm laser is 132 mW at the temperature of 20 ℃, and the power change is less than 2% in an hour. A periodically poled LiNbOa (PPLN) was used as outer cavity frequency-doubling crystal and 561-nm laser was observed.

  11. Picosecond holmium fibre laser pumped at 1125 \\ {\\text{nm}}

    Science.gov (United States)

    Kamynin, V. A.; Filatova, S. A.; Zhluktova, I. V.; Tsvetkov, V. B.

    2016-12-01

    We report a passively mode-locked, all-fibre holmium laser based on nonlinear polarisation rotation. As a pump source use is made of an 1125-{\\text{nm}} ytterbium-doped fibre laser. The pulse repetition rate of the holmium laser is 7.5 {\\text{MHz}}, and the pulse duration does not exceed 52 {\\text{ps}} at wavelengths of 2065 and 2080 {\\text{nm}}. The average laser output power reaches 5 {\\text{mW}}.

  12. 670-nm light treatment reduces complement propagation following retinal degeneration

    Directory of Open Access Journals (Sweden)

    Rutar Matt

    2012-11-01

    Full Text Available Abstract Aim Complement activation is associated with the pathogenesis of age-related macular degeneration (AMD. We aimed to investigate whether 670-nm light treatment reduces the propagation of complement in a light-induced model of atrophic AMD. Methods Sprague–Dawley (SD rats were pretreated with 9 J/cm2 670-nm light for 3 minutes daily over 5 days; other animals were sham treated. Animals were exposed to white light (1,000 lux for 24 h, after which animals were kept in dim light (5 lux for 7 days. Expression of complement genes was assessed by quantitative polymerase chain reaction (qPCR, and immunohistochemistry. Counts were made of C3-expressing monocytes/microglia using in situ hybridization. Photoreceptor death was also assessed using outer nuclear layer (ONL thickness measurements, and oxidative stress using immunohistochemistry for 4-hydroxynonenal (4-HNE. Results Following light damage, retinas pretreated with 670-nm light had reduced immunoreactivity for the oxidative damage maker 4-HNE in the ONL and outer segments, compared to controls. In conjunction, there was significant reduction in retinal expression of complement genes C1s, C2, C3, C4b, C3aR1, and C5r1 following 670 nm treatment. In situ hybridization, coupled with immunoreactivity for the marker ionized calcium binding adaptor molecule 1 (IBA1, revealed that C3 is expressed by infiltrating microglia/monocytes in subretinal space following light damage, which were significantly reduced in number after 670 nm treatment. Additionally, immunohistochemistry for C3 revealed a decrease in C3 deposition in the ONL following 670 nm treatment. Conclusions Our data indicate that 670-nm light pretreatment reduces lipid peroxidation and complement propagation in the degenerating retina. These findings have relevance to the cellular events of complement activation underling the pathogenesis of AMD, and highlight the potential of 670-nm light as a non-invasive anti-inflammatory therapy.

  13. Electron beam inspection methods for imprint lithography at 32 nm

    Science.gov (United States)

    Selinidis, Kosta; Thompson, Ecron; Sreenivasan, S. V.; Resnick, Douglas J.

    2009-01-01

    Step and Flash Imprint Lithography redefines nanoimprinting. This novel technique involves the field-by-field deposition and exposure of a low viscosity resist deposited by jetting technology onto the substrate. The patterned mask is lowered into the fluid which then quickly flows into the relief patterns in the mask by capillary action. Following this filling step, the resist is crosslinked under UV radiation, and then the mask is removed leaving a patterned solid on the substrate. Compatibility with existing CMOS processes requires a mask infrastructure in which resolution, inspection and repair are all addressed. The purpose of this paper is to understand the limitations of inspection at half pitches of 32 nm and below. A 32 nm programmed defect mask was fabricated. Patterns included in the mask consisted of an SRAM Metal 1 cell, dense lines, and dense arrays of pillars. Programmed defect sizes started at 4 nm and increased to 48 nm in increments of 4 nm. Defects in both the mask and imprinted wafers were characterized scanning electron microscopy and the measured defect areas were calculated. These defects were then inspected using a KLA-T eS35 electron beam wafer inspection system. Defect sizes as small as 12 nm were detected, and detection limits were found to be a function of defect type.

  14. Predicting lithography costs: guidance for <= 32 nm patterning solutions

    Science.gov (United States)

    Hazelton, Andrew J.; Wüest, Andrea; Hughes, Greg; Lercel, Michael

    2008-05-01

    Extending lithography to 32 nm and 22 nm half pitch requires the introduction of new lithography technologies, such as EUVL or high-index immersion, or new techniques, such as double patterning. All of these techniques introduce large changes into the single exposure immersion lithography process as used for the 45 nm half pitch node. Therefore, cost per wafer is a concern. In this paper, total patterning costs are estimated for the 32 nm and 22 nm half pitch nodes through the application of cost-of-ownership models based on the tool, mask, and process costs. For all cases, the cost of patterning at 32 nm half pitch for critical layers will be more expensive than in prior generations. Mask costs are observed to be a significant component of lithography costs even up to a mask usage of 10,000 wafers/mask in most cases. The more simple structure of EUVL masks reduces the mask cost component and results in EUVL being the most cost-effective patterning solution under the assumptions of high throughput and good mask blank defect density.

  15. Final report on EURAMET.M.P-K4.2010: Key and supplementary comparison of national pressure standards in the range 1 Pa to 15 kPa of absolute and gauge pressure

    Science.gov (United States)

    Krajíček, Zdeněk; Bergoglio, Mercede; Jousten, Karl; Otal, Pierre; Sabuga, Wladimir; Saxholm, Sari; Pražák, Dominik; Vičar, Martin

    2014-01-01

    This report describes a EURAMET comparison of five European National Metrology Institutes in low gauge and absolute pressure in gas (nitrogen), denoted as EURAMET.M.P-K4.2010. Its main intention is to state equivalence of the pressure standards, in particular those based on the technology of force-balanced piston gauges such as e.g. FRS by Furness Controls, UK and FPG8601 by DHI-Fluke, USA. It covers the range from 1 Pa to 15 kPa, both gauge and absolute. The comparison in absolute mode serves as a EURAMET Key Comparison which can be linked to CCM.P-K4 and CCM.P-K2 via PTB. The comparison in gauge mode is a supplementary comparison. The comparison was carried out from September 2008 till October 2012. The participating laboratories were the following: CMI, INRIM, LNE, MIKES, PTB-Berlin (absolute pressure 1 kPa and below) and PTB-Braunschweig (absolute pressure 1 kPa and above and gauge pressure). CMI was the pilot laboratory and provided a transfer standard for the comparison. This transfer standard was also the laboratory standard of CMI at the same time, which resulted in a unique and logistically difficult star comparison. Both in gauge and absolute pressures all the participating institutes successfully proved their equivalence with respect to the reference value and all also proved mutual bilateral equivalences in all the points. All the participating laboratories are also equivalent with the reference values of CCM.P-K4 and CCM.P-K2 in the relevant points. The comparison also proved the ability of FPG8601 to serve as a transfer standard. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  16. Regulation of Transcription of Nucleotide-Binding Leucine-Rich Repeat-Encoding Genes SNC1 and RPP4 via H3K4 Trimethylation1[C][W][OA

    Science.gov (United States)

    Xia, Shitou; Cheng, Yu Ti; Huang, Shuai; Win, Joe; Soards, Avril; Jinn, Tsung-Luo; Jones, Jonathan D.G.; Kamoun, Sophien; Chen, She; Zhang, Yuelin; Li, Xin

    2013-01-01

    Plant nucleotide-binding leucine-rich repeat (NB-LRR) proteins serve as intracellular sensors to detect pathogen effectors and trigger immune responses. Transcription of the NB-LRR-encoding Resistance (R) genes needs to be tightly controlled to avoid inappropriate defense activation. How the expression of the NB-LRR R genes is regulated is poorly understood. The Arabidopsis (Arabidopsis thaliana) suppressor of npr1-1, constitutive 1 (snc1) mutant carries a gain-of-function mutation in a Toll/Interleukin1 receptor-like (TIR)-NB-LRR-encoding gene, resulting in the constitutive activation of plant defense responses. A snc1 suppressor screen identified modifier of snc1,9 (mos9), which partially suppresses the autoimmune phenotypes of snc1. Positional cloning revealed that MOS9 encodes a plant-specific protein of unknown function. Expression analysis showed that MOS9 is required for the full expression of TIR-NB-LRR protein-encoding RECOGNITION OF PERONOSPORA PARASITICA 4 (RPP4) and SNC1, both of which reside in the RPP4 cluster. Coimmunoprecipitation and mass spectrometry analyses revealed that MOS9 associates with the Set1 class lysine 4 of histone 3 (H3K4) methyltransferase Arabidopsis Trithorax-Related7 (ATXR7). Like MOS9, ATXR7 is also required for the full expression of SNC1 and the autoimmune phenotypes in the snc1 mutant. In atxr7 mutant plants, the expression of RPP4 is similarly reduced, and resistance against Hyaloperonospora arabidopsidis Emwa1 is compromised. Consistent with the attenuated expression of SNC1 and RPP4, trimethylated H3K4 marks are reduced around the promoters of SNC1 and RPP4 in mos9 plants. Our data suggest that MOS9 functions together with ATXR7 to regulate the expression of SNC1 and RPP4 through H3K4 methylation, which plays an important role in fine-tuning their transcription levels and functions in plant defense. PMID:23690534

  17. 750 nm 1.5 W frequency-doubled semiconductor disk laser with a 44 nm tuning range.

    Science.gov (United States)

    Saarinen, Esa J; Lyytikäinen, Jari; Ranta, Sanna; Rantamäki, Antti; Sirbu, Alexei; Iakovlev, Vladimir; Kapon, Eli; Okhotnikov, Oleg G

    2015-10-01

    We demonstrate 1.5 W of output power at the wavelength of 750 nm by intracavity frequency doubling a wafer-fused semiconductor disk laser diode-pumped at 980 nm. An optical-to-optical efficiency of 8.3% was achieved using a bismuth borate crystal. The wavelength of the doubled emission could be tuned from 720 to 764 nm with an intracavity birefringent plate. The beam quality parameter M2 of the laser output was measured to be below 1.5 at all pump powers. The laser is a promising tool for biomedical applications that can take advantage of the large penetration depth of light in tissue in the 700-800 nm spectral range.

  18. Characteristics of THz Emission from GaAs Crystal Excited by 400 nm and 800 nm Optical Pulses

    Institute of Scientific and Technical Information of China (English)

    YANG Yu-Ping; XU Xin-Long; YAN Wei; WANG Li

    2005-01-01

    @@ THz emission spectroscopy is used to study the generation mechanism dependent behaviour of terahertz (THz) electromagnetic waves from the GaAs crystal under excitation by 400 nm and 800 nm femtosecond (fs) pulses,respectively. The wavelength dependence of the emission spectrum under two types of THz generation mechanisms is analysed. Under the optical rectification mechanism, a slight enhancement of the spectral amplitude in the high-frequency regime is observed in a GaAs(110) crystal by the excitation of a 400-nm optical pulse compared with that of 800nm. Whereas an obvious red shift of the amplitude spectrum occurs in the GaAs(100) sample under the transient photoconduction mechanism. These phenomena are explained in detail by the duration of the optical pump pulse and the band structure of GaAs, respectively.

  19. Imaging performance and challenges of 10nm and 7nm logic nodes with 0.33 NA EUV

    Science.gov (United States)

    van Setten, Eelco; Schiffelers, Guido; Psara, Eleni; Oorschot, Dorothe; Davydova, Natalia; Finders, Jo; Depre, Laurent; Farys, Vincent

    2014-10-01

    The NXE:3300B is ASML's third generation EUV system and has an NA of 0.33 and is positioned at a resolution of 22nm, which can be extended down to 18nm and below with off-axis illumination at full transmission. Multiple systems have been qualified and installed at customers. The NXE:3300B succeeds the NXE:3100 system (NA of 0.25), which has allowed customers to gain valuable EUV experience. It is expected that EUV will be adopted first for critical Logic layers at 10nm and 7nm nodes, such as Metal-1, to avoid the complexity of triple patterning schemes using ArF immersion. In this paper we will evaluate the imaging performance of (sub-)10nm node Logic M1 on the NXE:3300B EUV scanner. We will show the line-end performance of tip-to-tip and tip-to-space test features for various pitches and illumination settings and the performance enhancement obtained by means of a 1st round of OPC. We will also show the magnitude of local variations. The Logic M1 cell is evaluated at various critical features to identify hot spots. A 2nd round OPC model was calibrated of which we will show the model accuracy and ability to predict hot spots in the Logic M1 cell. The calibrated OPC model is used to predict the expected performance at 7nm node Logic using off-axis illumination at 16nm minimum half pitch. Initial results of L/S exposed on the NXE:3300B at 7nm node resolutions will be shown. An outlook is given to future 0.33 NA systems on the ASML roadmap with enhanced illuminator capabilities to further improve performance and process window.

  20. Crystal Structure of the 1:2 Stoichiometric Nd: [α-SiW11O39]8-: K5Na5H3[Nd(α-SiW11O39)2]·6H2O

    Institute of Scientific and Technical Information of China (English)

    HAN Qiu-Xia; WANG Jing-Ping; NIU Jing-Yang

    2007-01-01

    Single-crystal X-ray structural analysis of K5Na5H3[Nd(α-SiW11O39)2]·6H2O(triclinic, space group P1-, a = 13.7738(10), b = 19.722(2), c = 20.6647(15) (A), α = 111.0090(10), β =105.1740(10), γ = 98-6870(10)°, Z = 2, V = 4867.2(8), Mr = 5914.40, Dc = 4.026, μ = 26.754 mm-1,F(000) = 5076, R = 0.0750 and wR = 0.2077) reveals that two [α-SiW11O39]8- moieties are connected through one Nd atom which is in a square antiprismatic coordination environment by eight oxygen atoms from two [α-SiW11O391]8- moieties. The Nd(Ⅲ) ion is substituted for a [WO]4+unit in the "cap" region of the tungsten-oxygen framework of the parent Keggin ion. Disordered Na+cations and water molecules reside outside these channels, leaving empty pores with an intersecting slot (13 (A) × 10 (A)).

  1. Considerations for fine hole patterning for the 7nm node

    Science.gov (United States)

    Yaegashi, Hidetami; Oyama, Kenichi; Hara, Arisa; Natori, Sakurako; Yamauchi, Shohei; Yamato, Masatoshi; Koike, Kyohei

    2016-03-01

    One of the practical candidates to produce 7nm node logic devices is to use the multiple patterning with 193-immersion exposure. For the multiple patterning, it is important to evaluate the relation between the number of mask layer and the minimum pitch systematically to judge the device manufacturability. Although the number of the time of patterning, namely LE(Litho-Etch) ^ x-time, and overlay steps have to be reduced, there are some challenges in miniaturization of hole size below 20nm. Various process fluctuations on contact hole have a direct impact on device performance. According to the technical trend, 12nm diameter hole on 30nm-pitch hole will be needed on 7nm node. Extreme ultraviolet lithography (EUV) and Directed self-assembly (DSA) are attracting considerable attention to obtain small feature size pattern, however, 193-immersion still has the potential to extend optical lithography cost-effectively for sub-7nm node. The objective of this work is to study the process variation challenges and resolution in post-processing for the CD-bias control to meet sub-20nm diameter contact hole. Another pattern modulation is also demonstrated during post-processing step for hole shrink. With the realization that pattern fidelity and pattern placement management will limit scaling long before devices and interconnects fail to perform intrinsically, the talk will also outline how circle edge roughness (CER) and Local-CD uniformity can correct efficiency. On the other hand, 1D Gridded-Design-Rules layout (1D layout) has simple rectangular shapes. Also, we have demonstrated CD-bias modification on short trench pattern to cut grating line for its fabrication.

  2. Optical coherence tomography based imaging of dental demineralisation and cavity restoration in 840 nm and 1310 nm wavelength regions

    Science.gov (United States)

    Damodaran, Vani; Rao, Suresh Ranga; Vasa, Nilesh J.

    2016-08-01

    In this paper, a study of in-house built optical coherence tomography (OCT) system with a wavelength of 840 nm for imaging of dental caries, progress in demineralisation and cavity restoration is presented. The caries when imaged with the 840 nm OCT system showed minute demineralisation in the order of 5 μm. The OCT system was also proposed to study the growth of lesion and this was demonstrated by artificially inducing caries with a demineralisation solution of pH 4.8. The progress of carious lesion to a depth of about 50-60 μm after 60 hours of demineralisation was clearly observed with the 840 nm OCT system. The tooth samples were subjected to accelerated demineralisation condition at pH of approximately 2.3 to study the adverse effects and the onset of cavity formation was clearly observed. The restoration of cavity was also studied by employing different restorative materials (filled and unfilled). In the case of restoration without filler material (unfilled), the restoration boundaries were clearly observed. Overall, results were comparable with that of the widely used 1310 nm OCT system. In the case of restoration with filler material, the 1310 nm OCT imaging displayed better imaging capacity due to lower scattering than 840 nm imaging.

  3. Research of the Additional Losses Occurring in Optical Fiber at its Multiple Bends in the Range Waves 1310nm, 1550nm and 1625nm Long

    Science.gov (United States)

    Yurchenko, A. V.; Gorlov, N. I.; Alkina, A. D.; Mekhtiev, A. D.; Kovtun, A. A.

    2016-01-01

    Article is devoted to research of the additional losses occurring in the optical fiber at its multiple bends in the range waves of 1310 nanometers, 1550 nanometers and 1625 nanometers long. Article is directed on creation of the external factors methods which allow to estimate and eliminate negative influence. The automated way of calculation of losses at a bend is developed. Results of scientific researches are used by engineers of “Kazaktelekom” AS for practical definition of losses service conditions. For modeling the Wolfram|Alpha environment — the knowledge base and a set of computing algorithms was chosen. The greatest losses are noted on wavelength 1310nm and 1625nm. All dependences are nonlinear. Losses with each following excess are multiplicative.

  4. Observation of Quiet Limb in He I 1083.0 nm, H Paschen alpha1281.8 nm and H Brackett gamma 2166.1 nm lines

    Science.gov (United States)

    Prasad Choudhary, Debi

    2016-05-01

    In this paper, we shall present the results of an observational study of the quiet solar limb in the near infrared lines using the New IR Array Camera (NAC) and the vertical spectrograph at the focal plane of McMath-Pierce telescope. The NAC, at the exit port of the spectrograph, was used to record the limb spectrum in HeI 1083.0 nm, Hydrogen Paschen 1281.8 nm and Brackett 2165.5 nm wavelength regions. The NAC is a 1024x1024 InSb Alladin III Detector operating over 1-5 micron range with high density sampling at 0.018 arc second/pixel. The all-reflective optical train minimizes number of surfaces and eliminates ghosts leading to low scatter, ghost-free optics. The close-cycle cryogenic provides a stable cooling environment over six hour period with an accuracy of 0.01K leading to low dark current. The low read out noise combined with low scattered light and dark current makes NAC an ideal detector for making high quality infrared spectral observations of solar limb. The limb spectrums were obtained by placing the spectrograph slit perpendicular to the limb at an interval of 10 degrees around the solar disk. We shall report the intensity profile, line-of-sight velocity and line width distribution around the sun derived from the spectra along the slit.

  5. H2A.Z demarcates intergenic regions of the plasmodium falciparum epigenome that are dynamically marked by H3K9ac and H3K4me3.

    Directory of Open Access Journals (Sweden)

    Richárd Bártfai

    Full Text Available Epigenetic regulatory mechanisms and their enzymes are promising targets for malaria therapeutic intervention; however, the epigenetic component of gene expression in P. falciparum is poorly understood. Dynamic or stable association of epigenetic marks with genomic features provides important clues about their function and helps to understand how histone variants/modifications are used for indexing the Plasmodium epigenome. We describe a novel, linear amplification method for next-generation sequencing (NGS that allows unbiased analysis of the extremely AT-rich Plasmodium genome. We used this method for high resolution, genome-wide analysis of a histone H2A variant, H2A.Z and two histone H3 marks throughout parasite intraerythrocytic development. Unlike in other organisms, H2A.Z is a constant, ubiquitous feature of euchromatic intergenic regions throughout the intraerythrocytic cycle. The almost perfect colocalisation of H2A.Z with H3K9ac and H3K4me3 suggests that these marks are preferentially deposited on H2A.Z-containing nucleosomes. By performing RNA-seq on 8 time-points, we show that acetylation of H3K9 at promoter regions correlates very well with the transcriptional status whereas H3K4me3 appears to have stage-specific regulation, being low at early stages, peaking at trophozoite stage, but does not closely follow changes in gene expression. Our improved NGS library preparation procedure provides a foundation to exploit the malaria epigenome in detail. Furthermore, our findings place H2A.Z at the cradle of P. falciparum epigenetic regulation by stably defining intergenic regions and providing a platform for dynamic assembly of epigenetic and other transcription related complexes.

  6. KdmB, a Jumonji Histone H3 Demethylase, Regulates Genome-Wide H3K4 Trimethylation and Is Required for Normal Induction of Secondary Metabolism in Aspergillus nidulans.

    Directory of Open Access Journals (Sweden)

    Agnieszka Gacek-Matthews

    2016-08-01

    Full Text Available Histone posttranslational modifications (HPTMs are involved in chromatin-based regulation of fungal secondary metabolite biosynthesis (SMB in which the corresponding genes-usually physically linked in co-regulated clusters-are silenced under optimal physiological conditions (nutrient-rich but are activated when nutrients are limiting. The exact molecular mechanisms by which HPTMs influence silencing and activation, however, are still to be better understood. Here we show by a combined approach of quantitative mass spectrometry (LC-MS/MS, genome-wide chromatin immunoprecipitation (ChIP-seq and transcriptional network analysis (RNA-seq that the core regions of silent A. nidulans SM clusters generally carry low levels of all tested chromatin modifications and that heterochromatic marks flank most of these SM clusters. During secondary metabolism, histone marks typically associated with transcriptional activity such as H3 trimethylated at lysine-4 (H3K4me3 are established in some, but not all gene clusters even upon full activation. KdmB, a Jarid1-family histone H3 lysine demethylase predicted to comprise a BRIGHT domain, a zinc-finger and two PHD domains in addition to the catalytic Jumonji domain, targets and demethylates H3K4me3 in vivo and mediates transcriptional downregulation. Deletion of kdmB leads to increased transcription of about ~1750 genes across nutrient-rich (primary metabolism and nutrient-limiting (secondary metabolism conditions. Unexpectedly, an equally high number of genes exhibited reduced expression in the kdmB deletion strain and notably, this group was significantly enriched for genes with known or predicted functions in secondary metabolite biosynthesis. Taken together, this study extends our general knowledge about multi-domain KDM5 histone demethylases and provides new details on the chromatin-level regulation of fungal secondary metabolite production.

  7. Atmospheric sub-3 nm particles at high altitudes

    Directory of Open Access Journals (Sweden)

    S. Mirme

    2010-01-01

    Full Text Available Formation of new atmospheric aerosol particles is known to occur almost all over the world and the importance of these particles to climate and air quality has been recognized. Recently, it was found that atmospheric aerosol particle formation begins at the diameter of around 1.5–2.0 nm and a pool of sub-3 nm atmospheric particles – consisting of both charged and uncharged ones – was observed at the ground level. Here, we report on the first airborne observations of the pool of sub-3 nm neutral atmospheric particles. Between 2 and 3 nm, their concentration is roughly two orders of magnitude larger than that of the ion clusters, depending slightly on the altitude. Our findings indicate that new particle formation takes place throughout the tropospheric column up to the tropopause. Particles were found to be formed via neutral pathways in the boundary layer, and there was no sign of an increasing role by ion-induced nucleation toward the upper troposphere. Clouds, while acting as a source of sub-10 nm ions, did not perturb the overall budget of atmospheric clusters or particles.

  8. Atmospheric sub-3 nm particles at high altitudes

    Directory of Open Access Journals (Sweden)

    S. Mirme

    2009-09-01

    Full Text Available Formation of new atmospheric aerosol particles is known to occur almost all over the world and the importance of these particles to climate and air quality has been recognized. Recently, it was found that atmospheric aerosol formation begins at particle diameter of around 1.5–2.0 nm and a pool of sub-3 nm atmospheric particles – consisting of both charged and uncharged ones – was observed at the ground level. Here, we report on the first airborne observations of the pool of sub-3 nm neutral atmospheric particles. Between 2 and 3 nm, their concentration is roughly two orders of magnitude larger than that of the ion clusters, depending slightly on the altitude. Our findings indicate that new particle formation takes place actively throughout the tropospheric column up to the tropopause. Particles were found to be formed via neutral pathways in the boundary layer, and there was no sign of an increasing role by ion-induced nucleation toward the upper troposphere. Clouds, while acting as a source of sub-10 nm ions, did not perturb the overall budget of atmospheric clusters or particles.

  9. Switching Properties of sub-100 nm Perpendicular Magnetic Tunnel Junctions

    Science.gov (United States)

    Tryputen, Larysa; Piotrowski, Stephan; Bapna, Mukund; Chien, Chia-Ling; Wang, Weigang; Majetich, Sara; Ross, Caroline

    2015-03-01

    Perpendicular magnetic tunnel junctions (p-MTJs) have great potential for realizing high-density non-volatile memory and logic devices. It is critical to solve scalability problem to implement such devices, to achieve low resistance area and to reduce switching current density while maintaining thermal stability. We present our recent results on fabrication of high resolution Ta/CoFeB/MgO/CoFeB/Ta p-MTJ devices and characterization of their switching properties as well as topography and current mapping by using nanoscale Conductive Atomic Force Microscopy. Our patterning method is based on using hydrogen silsesquioxane resist mask combined with ion beam etching. It allows to fabricate p-MTJ devices down to 40 nm in diameter while maintaining the magnetic quality of the multilayers. Repeatable, consistent switching behaviour has been observed in the obtained p-MTJ devices of 500 nm down to 40 nm with 10 - 800 mV voltage applied. Switching field increased as device diameter decreased, from 580 Oe at 500 nm (MR = 10%) to 410 Oe at 80 nm (MR = 9%). We discuss the effect of device sizes on the switching properties. This work was supported in part by C-SPIN, one of the six centers of STARnet, a Semiconductor Research Corporation Program sponsored by MARCO and DARPA and in part through the National Science Foundation through NCN-Needs Program, Contract 12207020-EEC.

  10. Ultraviolet photodissociation of 1-bromopropane at 234 and 267 nm

    Science.gov (United States)

    Zhang, Song; Wang, Yanmei; Tang, Bifeng; Zheng, Qiusha; Zhang, Bing

    2005-09-01

    The photodissociation of 1-bromopropane was investigated at 234 and 267 nm utilizing ion velocity imaging method. Both the speed and angular distributions of Br*( 2P 1/2) and Br( 2P 3/2) fragments were determined. β(Br) = 0.77 ± 0.07 and β(Br*)= 1.68 ± 0.06 at 234 nm; β(Br) = 0.38 ± 0.02 and β(Br*) = 1.33 ± 0.09 at 267 nm were observed. The total translational energy distributions were single Gaussian distribution and interpreted using soft or rigid implusive mode at different wavelength, respectively. The experiment also shows that Br is dominant in the dissociation and originates mostly from 3A', while Br* corresponds to 4A' ← 3A' coupling.

  11. High Reflectivity of Silver Extended Down to 200 NM

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, N L; Wolfe, J D

    2001-06-04

    Silver has the highest reflectance of all of the metals, but it tarnishes in the presence of sulfides, chlorides, and oxides in the atmosphere. Also, the silver reflectance is very low at wavelengths below 400 nm making aluminum more desirable mirror coating for the W region. We have found a way to prevent silver tarnishing by sandwiching the silver layer between two thin layers of NiCrN{sub x}, and to extend the metal's high reflectance down to 200 nm by depositing the (thin) Ag layer on top of Al. Thus, the uv is transmitted through the thin Ag layer below 400 nm wavelength, and is reflected from the A1 layer underneath. This W-shifted durable coating provides a valuable alternative to the aluminum coating for telescope mirror coatings where collection efficiency is an important consideration.

  12. Challenges of 22 nm and beyond CMOS technology

    Institute of Scientific and Technical Information of China (English)

    HUANG Ru; WU HanMing; KANG JinFeng; XIAO DeYuan; SHI XueLong; AN Xia; TIAN Yu; WANG RunSheng; ZHANG LiangLiang; ZHANG Xing; WANG YangYuan

    2009-01-01

    It is predicted that CMOS technology will probably enter Into 22 nm node around 2012.Scaling of CMOS logic technology from 32 to 22 nm node meets more critical Issues and needs some significant changes of the technology,as well as integration of the advanced processes.This paper will review the key processing technologies which can be potentially integrated into 22 nm and beyond technology nodes,including double patterning technology with high NA water immersion lithography and EUV lithography,new device architectures,high K/metal gate (HK/MG) stack and integration technology,mobility enhancement technologies,source/drain engineering and advanced copper interconnect technology with ultra-low-k process.

  13. Photoluminescence of single colour defects in 50nm diamond nanocrystals

    Energy Technology Data Exchange (ETDEWEB)

    Treussart, F. [Laboratoire de Photonique Quantique et Moleculaire, UMR 8537 du CNRS, ENS Cachan, 61 avenue du President Wilson, 94235 Cachan cedex (France)]. E-mail: francois.treussart@physique.ens-cachan.fr; Jacques, V. [Laboratoire de Photonique Quantique et Moleculaire, UMR 8537 du CNRS, ENS Cachan, 61 avenue du President Wilson, 94235 Cachan cedex (France); Wu, E. [Laboratoire de Photonique Quantique et Moleculaire, UMR 8537 du CNRS, ENS Cachan, 61 avenue du President Wilson, 94235 Cachan cedex (France); Gacoin, T. [Laboratoire de Physique de la Matiere Condensee, UMR 7643 du CNRS, Ecole Polytechnique, 91128 Palaiseau cedex (France); Grangier, P. [Laboratoire Charles Fabry de l' Institut d' Optique, UMR 8501 du CNRS, BP 147, 91403 Orsay cedex (France); Roch, J.-F. [Laboratoire de Photonique Quantique et Moleculaire, UMR 8537 du CNRS, ENS Cachan, 61 avenue du President Wilson, 94235 Cachan cedex (France)

    2006-04-01

    We used optical confocal microscopy to study optical properties of diamond 50nm nanocrystals first irradiated with an electron beam, then dispersed as a colloidal solution and finally deposited on a silica slide. At room temperature, under CW laser excitation at a wavelength of 514.5nm we observed perfectly photostable single nitrogen-vacancy (NV) colour defects embedded in the nanocrystals. From the zero-phonon line around 575nm in the spectrum of emitted light, we infer a neutral NV{sup 0} type of defect. Such nanoparticle with intrinsic fluorescence are highly promising for applications in biology where long-term emitting fluorescent bio-compatible nanoprobes are still missing.

  14. 30 Gbps bottom-emitting 1060 nm VCSEL

    DEFF Research Database (Denmark)

    Tatarczak, Anna; Zheng, Y.; Rodes, G. A.

    2014-01-01

    1060 nm VCSEL-based data transmission over 50 m OM3 MMF at 30 Gbit/s is experimentally demonstrated. A highly-strained bottom-emitting QW VCSEL with p-type modulation doping is used with 3.77 mA bias and 0.55 V data amplitude.......1060 nm VCSEL-based data transmission over 50 m OM3 MMF at 30 Gbit/s is experimentally demonstrated. A highly-strained bottom-emitting QW VCSEL with p-type modulation doping is used with 3.77 mA bias and 0.55 V data amplitude....

  15. Epitaxy and Characteristics of Resonant Cavity LEDs at 650 nm

    Institute of Scientific and Technical Information of China (English)

    Kang Yuzhu; Li Jianjun; Ding Liang; Yang Zhen; Han Jun; Deng Jun; Zou Deshu; Shen Guangdi

    2009-01-01

    Resonant-cavity light-emitting diodes (RCLED) at 650 nm wavelength were grown by metal organic chemical vapor deposition. In order to improve the interface quality and reduce the device voltage, an AlGaInP material system has been chosen to grow the top DBRs. The emission properties of the RCLED were characterized by measuring PL and EL spectra. The average emission power of the device is 0.5 mW at 20 mA and 2.2 V, and its spectrum full width at half maximum is about 10 nm.

  16. Injection molding of high aspect ratio sub-100 nm nanostructures

    DEFF Research Database (Denmark)

    Matschuk, Maria; Larsen, Niels B

    2013-01-01

    with FDTS. Reduced adhesion forces are consistent with lowered friction that reduces the risk of fracturing the nanoscopic pillars during demolding. Optimized mold surface chemistry and associated injection molding conditions permitted the fabrication of square arrays of 40 nm wide and 107 nm high (aspect......We have explored the use of mold coatings and optimized processing conditions to injection mold high aspect ratio nanostructures (height-to-width >1) in cyclic olefin copolymer (COC). Optimizing the molding parameters on uncoated nickel molds resulted in slight improvements in replication quality...

  17. Platelet-Activating Factor Blockade Inhibits the T-Helper Type 17 Cell Pathway and Suppresses Psoriasis-Like Skin Disease in K5.hTGF-β1 Transgenic Mice

    Science.gov (United States)

    Singh, Tej Pratap; Huettner, Barbara; Koefeler, Harald; Mayer, Gerlinde; Bambach, Isabella; Wallbrecht, Katrin; Schön, Michael P.; Wolf, Peter

    2011-01-01

    Platelet-activating factor (PAF), a potent biolipid mediator, is involved in a variety of cellular transduction pathways and plays a prominent role in inducing inflammation in different organs. We used K5.hTGF-β1 transgenic mice, which exhibit an inflammatory skin disorder and molecular and cytokine abnormalities with strong similarities to human psoriasis, to study the pathogenic role of PAF. We found that injecting PAF into the skin of transgenic mice led to inflammation and accelerated manifestation of the psoriatic phenotype by a local effect. In contrast, injecting mice with PAF receptor antagonist PCA-4248 lowered the PAF level (most likely by depressing an autocrine loop) and neutrophil, CD68+ cell (monocyte/macrophage), and CD3+ T-cell accumulation in the skin and blocked progression of the psoriasis-like phenotype. This effect of PAF blockade was specific and similar to that of psoralen–UV-A and was paralleled by a decrease in abnormally elevated mRNA and/or protein levels of T-helper type 17 cell–related cytokines IL-17A, IL-17F, IL-23, IL-12A, and IL-6 and its transcription factor signal transducer and activator of transcription 3. In contrast, PCA-4248 treatment up-regulated mRNA levels of cyclooxygenase-2 and IL-10 in dorsal skin and release of IL-10 in serum and skin. Interfering with PAF may offer the opportunity to develop novel therapeutic strategies for inflammatory psoriasis and associated comorbidities, including metabolic syndrome and atherosclerosis, in which the IL-17 axis may be involved. PMID:21281802

  18. 785nm dual-wavelength Y-branch DBR-RW diode laser with electrically adjustable wavelength distance between 0 nm and 2 nm

    Science.gov (United States)

    Sumpf, Bernd; Kabitzke, Julia; Fricke, Jörg; Ressel, Peter; Müller, André; Maiwald, Martin; Tränkle, Günther

    2017-02-01

    Shifted excitation Raman difference spectroscopy is a powerful tool to separate the weak Raman lines from disturbing background light like fluorescence, day light or artificial light. When exciting the sample alternatingly with two slightly shifted wavelengths, the Raman lines follow the change whereas the background remains unchanged. Therefore, background free Raman spectra can be obtained measuring the two Raman spectra, subtracting the two signals and applying a reconstruction algorithm. When the spectral distance between the two wavelengths is the width of the Raman lines under study best signal-to-noise ratios can be achieved. In this work, monolithic dual wavelength Y-branch DBR ridge waveguide diode lasers with resistor heaters over the DBR gratings will be presented. The devices have a total length of 3 mm and a RW stripe width of 2.2 μm. The wavelengths are defined and stabilized using 500 μm long 10th order gratings with a designed spectral distance of 0.62 nm. Using the resistor heaters, this distance can be adjusted. The monolithic devices reach optical output powers up to 180 mW. Over the full range, they operate in single mode. The emission width is smaller than 13 pm (FWHM). At an output power of 50 mW the conversion efficiency is 0.22, which only slightly decreases down to 0.18 at maximal power. At an output power of 100 mW and with heater currents smaller than 600 mA, the spectral distance can be tuned from 0 nm up to 2 nm. The spectra remain single mode.

  19. High power, continuous-wave, single frequency fiber amplifier at 1091 nm and frequency doubling to 545.5 nm

    CERN Document Server

    Stappel, M; Kolbe, D; Walz, J

    2012-01-01

    We present a high power single-frequency ytterbium fiber amplifier system with an output power of 30 W at 1091 nm. The amplifier system consists of two stages, a preamplifier stage in which amplified spontaneous emission is efficiently suppressed (>40 dB) and a high power amplifier with an efficiency of 52 %. Two different approaches of frequency doubling are compared. We achieve 8.6 W at 545.5 nm by single-pass frequency doubling in a MgO-doped periodically poled stoichiometric LiTaO3 and up to 19.3 W at 545.5 nm by frequency doubling with a lithium-triborate (LBO) crystal in an external enhancement cavity.

  20. Low-noise design issues for analog front-end electronics in 130 nm and 90 nm CMOS technologies

    CERN Document Server

    Manghisoni, M; Re, V; Speziali, V; Traversi, G

    2007-01-01

    Deep sub-micron CMOS technologies provide wellestablished solutions to the implementation of low-noise front-end electronics in various detector applications. The IC designers’ effort is presently shifting to 130 nm CMOS technologies, or even to the next technology node, to implement readout integrated circuits for silicon strip and pixel detectors, in view of future HEP applications. In this work the results of noise measurements carried out on CMOS devices in 130 nm and 90 nm commercial processes are presented. The behavior of the 1/f and white noise terms is studied as a function of the device polarity and of the gate length and width. The study is focused on low current density applications where devices are biased in weak or moderate inversion. Data obtained from the measurements provide a powerful tool to establish design criteria in nanoscale CMOS processes for detector front-ends in LHC upgrades.

  1. On-chip measurements of Brownian relaxation of magnetic beads with diameters from 10 nm to 250 nm

    DEFF Research Database (Denmark)

    Østerberg, Frederik Westergaard; Rizzi, Giovanni; Hansen, Mikkel Fougt

    2013-01-01

    We demonstrate the use of planar Hall effect magnetoresistive sensors for AC susceptibility measurements of magnetic beads with frequencies ranging from DC to 1 MHz. This wide frequency range allows for measuring Brownian relaxation of magnetic beads with diameters ranging from 10 nm to 250 nm...... to sedimentation, magnetic trapping, and signal per bead. Among the investigated beads, we conclude that the beads with a nominal diameter of 80 nm are best suited for future on-chip volume-based biosensing experiments using planar Hall effect sensors........ Brownian relaxation is measured for six different magnetic bead types and their hydrodynamic diameters are determined. The hydrodynamic diameters are found to be within 40% of the nominal bead diameters. We discuss the applicability of the different bead types for volume-based biosensing with respect...

  2. Characterization of single 1.8-nm Au nanoparticle attachments on AFM tips for single sub-4-nm object pickup.

    Science.gov (United States)

    Cheng, Hui-Wen; Chang, Yuan-Chih; Tang, Song-Nien; Yuan, Chi-Tsu; Tang, Jau; Tseng, Fan-Gang

    2013-11-15

    This paper presents a novel method for the attachment of a 1.8-nm Au nanoparticle (Au-NP) to the tip of an atomic force microscopy (AFM) probe through the application of a current-limited bias voltage. The resulting probe is capable of picking up individual objects at the sub-4-nm scale. We also discuss the mechanisms involved in the attachment of the Au-NP to the very apex of an AFM probe tip. The Au-NP-modified AFM tips were used to pick up individual 4-nm quantum dots (QDs) using a chemically functionalized method. Single QD blinking was reduced considerably on the Au-NP-modified AFM tip. The resulting AFM tips present an excellent platform for the manipulation of single protein molecules in the study of single protein-protein interactions.

  3. InGaN light emitting diodes for 415 nm-520 nm spectral range by plasma assisted MBE

    Energy Technology Data Exchange (ETDEWEB)

    Szankowska, M.L.; Smalc-Koziorowska, J.; Cywinski, G.; Grzanka, S.; Grzegory, I.; Lucznik, B. [Institute of High Pressure Physics, Polish Academy of Sciences, 01-142 Warszawa (Poland); Feduniewicz-Zmuda, A. [TopGaN Ltd, ul Sokolowska 29/37, 01-142 Warszawa (Poland); Wasilewski, Z.R. [Institute for Microstructural Sciences, National Research Council, Ottawa (Canada); Porowski, S.; Skierbiszewski, C. [Institute of High Pressure Physics, Polish Academy of Sciences, 01-142 Warszawa (Poland); TopGaN Ltd, ul Sokolowska 29/37, 01-142 Warszawa (Poland); Siekacz, M

    2009-06-15

    In this work we study the growth of the Light Emitting Diodes (LEDs) by Plasma Assisted MBE (PAMBE). The active LEDs region was grown to cover the spectral range spanning from 415 nm to 520 nm. We demonstrate efficient LEDs with the highest optical power output of 1.5 mW and 20 mA for 415 nm. For longer wavelengths we observe a drop of the optical power. The reduction of the quantum efficiency for green emission can be related to the presence of strong built-in piezoelectric fields or increased number of nonradiative recombination centers. (copyright 2009 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  4. First results from simultaneous 527 nm and 351 nm probe beam interactions in a long scalelength plasma

    Science.gov (United States)

    Moody, J. D.; MacKinnon, A.; Glenzer, S. H.; Froula, D.; Gregori, G.; Berger, R. L.; Campbell, K.; Divol, L.; Dixit, S.; Suter, L. J.; Williams, E. A.; Bahr, R.; Seka, W.

    2002-11-01

    We investigate the stimulated Raman and Brillouin backscattered light from simultaneous 527 nm and 351 nm probe beams incident on a long scalelength ignition-like plasma. These experiments are important for both determining backscattering physics mechanisms and for evaluating laser power loss expected in planned ignition experiments. The plasma is formed using 18 kJ of 351 nm light from the Omega laser in a 1 ns pulse incident on a gas-filled balloon target. The two probe beams, which are delayed 0.5 ns relative to the plasma forming beams, are separated by 42^rc, have vacuum intensity of CO2 plasma. We describe the experimental results and simulations using the LASNEX hydrodynamic code and the pF3D laser-plasma wave propagation code. Work performed under the auspicies of the U.S. Department of Energy by the Lawrence Livermore National Laboratory under contract number W--7405--ENG--48.

  5. Imaging challenges in 20nm and 14nm logic nodes: hot spots performance in Metal1 layer

    Science.gov (United States)

    Timoshkov, V.; Rio, D.; Liu, H.; Gillijns, W.; Wang, J.; Wong, P.; Van Den Heuvel, D.; Wiaux, V.; Nikolsky, P.; Finders, J.

    2013-10-01

    The 20nm Metal1 layer, based on ARM standard cells, has a 2D design with minimum pitch of 64nm. This 2D design requires a Litho-Etch-Litho-Etch (LELE) double patterning. The whole design is divided in 2 splits: Me1A and Me1B. But solution of splitting conflicts needs stitching at some locations, what requires good Critical Dimension (CD) and overlay control to provide reliable contact between 2 stitched line ends. ASML Immersion NXT tools are aimed at 20 and 14nm logic production nodes. Focus control requirements become tighter, as existing 20nm production logic layouts, based on ARM, have about 50-60nm focus latitude and tight CD Uniformity (CDU) specifications, especially for line ends. IMEC inspected 20nm production Metal1 ARM standard cells with a Negative Tone Development (NTD) process using the Process Window Qualification-like technique experimentally and by Brion Tachyon LMC by simulations. Stronger defects were found thru process variations. A calibrated Tachyon model proved a good overall predictability capability for this process. Selected defects are likely to be transferred to hard mask during etch. Further, CDU inspection was performed for these critical features. Hot spots showed worse CD uniformity than specifications. Intra-field CDU contribution is significant in overall CDU budget, where reticle has major impact due to high MEEF of hot spots. Tip-to-Tip and tip-to-line hot spots have high MEEF and its variation over the field. Best focus variation range was determined by best focus offsets between hot spots and its variation within the field.

  6. Red (660 nm) or near-infrared (810 nm) photobiomodulation stimulates, while blue (415 nm), green (540 nm) light inhibits proliferation in human adipose-derived stem cells.

    Science.gov (United States)

    Wang, Yuguang; Huang, Ying-Ying; Wang, Yong; Lyu, Peijun; Hamblin, Michael R

    2017-08-10

    We previously showed that blue (415 nm) and green (540 nm) wavelengths were more effective in stimulating osteoblast differentiation of human adipose-derived stem cells (hASC), compared to red (660 nm) and near-infrared (NIR, 810 nm). Intracellular calcium was higher after blue/green, and could be inhibited by the ion channel blocker, capsazepine. In the present study we asked what was the effect of these four wavelengths on proliferation of the hASC? When cultured in proliferation medium there was a clear difference between blue/green which inhibited proliferation and red/NIR which stimulated proliferation, all at 3 J/cm(2). Blue/green reduced cellular ATP, while red/NIR increased ATP in a biphasic manner. Blue/green produced a bigger increase in intracellular calcium and reactive oxygen species (ROS). Blue/green reduced mitochondrial membrane potential (MMP) and lowered intracellular pH, while red/NIR had the opposite effect. Transient receptor potential vanilloid 1 (TRPV1) ion channel was expressed in hADSC, and the TRPV1 ligand capsaicin (5uM) stimulated proliferation, which could be abrogated by capsazepine. The inhibition of proliferation caused by blue/green could also be abrogated by capsazepine, and by the antioxidant, N-acetylcysteine. The data suggest that blue/green light inhibits proliferation by activating TRPV1, and increasing calcium and ROS.

  7. EST Table: NM_001170997 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001170997 BLOS2 10/09/29 91 %/145 aa ref|NP_001164468.1| biogenesis of lysosome-related organelles... complex 1 subunit 2 [Bombyx mori] dbj|BAI63077.1| biogenesis of lysosome-related organelles

  8. EST Table: NM_001043844 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001043844 VG 10/09/29 97 %/1782 aa ref|NP_001037309.1| vitellogenin precursor [B...: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  9. EST Table: NM_001043517 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001043517 Cycb 10/09/29 89 %/700 aa ref|NP_001036982.1| Cycle like factor b [Bom...byx mori] dbj|BAB91178.1| Cycle like factor BmCyc b [Bombyx mori] 10/09/13 52 %/387 aa FBpp0114038|DanaGF108

  10. EST Table: NM_001130897 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001130897 CCAP 11/12/09 n.h 10/09/29 100 %/103 aa ref|NP_001124369.1| crustacean... cardioactive peptide [Bombyx mori] dbj|BAG50376.1| crustacean cardioactive peptide [Bombyx mori] 10/09/13 l

  11. Optically pumped 1550nm wavelength tunable MEMS VCSEL

    DEFF Research Database (Denmark)

    Sahoo, Hitesh Kumar; Ansbæk, Thor; Ottaviano, Luisa

    2016-01-01

    The paper presents the design and fabrication of an optically pumped 1550nm tunable MEMS VCSEL with anenclosed MEMS. The MEMS is defined in SOI and the active material, an InP wafer with quantum wells arebonded to the SOI and the last mirror is made from the deposition of dielectric materials...

  12. EST Table: NM_001043468 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001043468 Mf-cpa 10/09/29 91 %/479 aa ref|NP_001036933.1| molting fluid carboxyp...eptidase A [Bombyx mori] dbj|BAD60916.1| molting fluid carboxypeptidase A [Bombyx mori] 10/09/13 50 %/393 aa

  13. EST Table: NM_001044201 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001044201 Tert 10/09/29 100 %/703 aa ref|NP_001037666.1| telomerase reverse tran...scriptase [Bombyx mori] gb|ABC95023.1| telomerase reverse transcriptase [Bombyx mori] gb|ABF56516.1| telomerase reverse

  14. EST Table: NM_001142927 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001142927 Kynu 10/09/29 100 %/426 aa ref|NP_001136399.1| kynureninase [Bombyx mori] dbj|BAH03383.1| kynur...eninase [Bombyx mori] 10/09/13 n.h 10/08/29 32 %/432 aa C15H9.7#CE06835#WBGene00015802#kynure

  15. EST Table: NM_001112748 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001112748 Cyp314a1 10/09/29 95 %/516 aa ref|NP_001106219.1| ecdysone 20-hydroxyl...ase [Bombyx mori] dbj|BAE45332.1| ecdysone 20-hydroxylase [Bombyx mori] 10/09/13 48 %/475 aa FBpp0206947|Dse

  16. Photodegradation of perfluorooctanoic acid by 185 nm vacuum ultraviolet light

    Institute of Scientific and Technical Information of China (English)

    CHEN Jing; ZHANG Peng-yi; LIU Jian

    2007-01-01

    The photodegradation of persistent and bioaccumulative perfluorooctanoic acid (PFOA) in water by 185 nm vacuum ultraviolet (VUV) light was examined to develop an effective technology to deal with PFOA pollution. PFOA degraded very slowly under irradiation of 254 nm UV light. However, 61.7% of initial PFOA was degraded by 185 nm VUV light within 2 h, and defluorination ratio reached 17.1%. Pseudo first-order-kinetics well simulated its degradation and defluorination. Besides, fluoride ion formed in water, 4 shorter-chain perfluorinated carboxylic acids (PFCAs), that is, perfluoroheptanoic acid, perfluorohexanoic acid, perfluoropentanoic acid, and perfluorobutanoic acid. These were identified as intermediates by LC-MS measurement. These PFCAs consecutively formed and further degraded with irradiation time. According to the mass balance calculation, no other byproducts were formed. It was proposed that PFCAs initially are decarboxylated by 185 nm light, and the radical thus formed reacts with water to form shorter-chain PFCA with one less CF2 unit.

  17. EST Table: NM_001099846 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001099846 LOC100101223 10/09/29 87 %/371 aa ref|NP_001093316.1| adiponectin rece...ptor [Bombyx mori] gb|ABK57116.2| adiponectin receptor [Bombyx mori] 10/09/13 75 %/318 aa FBpp0171941|DmojGI

  18. EST Table: NM_001043951 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001043951 Ash1 10/09/29 86 %/188 aa ref|NP_001037416.1| achaete-scute-like prote...in [Bombyx mori] gb|ABC84346.1| achaete-scute-like protein [Bombyx mori] gb|ABC84347.1| achaete-scute-like p

  19. EST Table: NM_001105226 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001105226 Ase 10/09/29 93 %/404 aa ref|NP_001098696.1| achaete-scute-like protei...n ASE [Bombyx mori] gb|ABR20841.1| achaete-scute-like protein ASE [Bombyx mori] 10/09/13 low homology 10/08/

  20. FDML swept source at 1060 nm using a tapered amplifier

    DEFF Research Database (Denmark)

    Marschall, Sebastian; Klein, Thomas; Wieser, Wolfgang;

    2010-01-01

    in this wavelength range. We demonstrate that a tapered amplifier can be integrated into a fiber-based swept source and allows for high-speed FDML operation. The developed light source operates at a sweep rate of 116kHz with an effective average output power in excess of 30mW. With a total sweep range of 70 nm...

  1. 78 FR 67210 - Santa Clara Pueblo Disaster #NM-00038

    Science.gov (United States)

    2013-11-08

    ... ADMINISTRATION Santa Clara Pueblo Disaster NM-00038 AGENCY: U.S. Small Business Administration. ACTION: Notice...: Submit completed loan applications to: U.S. Small Business Administration, Processing and Disbursement... of Disaster Assistance, U.S. Small Business Administration, 409 3rd Street, SW., Suite...

  2. Benchmarking of 50 nm features in thermal nanoimprint

    DEFF Research Database (Denmark)

    Gourgon, C.; Chaix, N.; Schift, H.;

    2007-01-01

    The objective of this benchmarking is to establish a comparison of several tools and processes used in thermal NIL with Si stamps at the nanoscale among the authors' laboratories. The Si stamps have large arrays of 50 nm dense lines and were imprinted in all these laboratories in a similar to 100...

  3. EST Table: NM_001046878 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001046878 LOC732875 10/09/29 100 %/169 aa ref|NP_001040343.1| peripheral-type benzodiazepine... receptor [Bombyx mori] gb|ABF51223.1| peripheral-type benzodiazepine receptor [Bombyx mori] 10/

  4. Direct visualization of fluid dynamics in sub-10 nm nanochannels.

    Science.gov (United States)

    Li, Huawei; Zhong, Junjie; Pang, Yuanjie; Zandavi, Seyed Hadi; Persad, Aaron Harrinarine; Xu, Yi; Mostowfi, Farshid; Sinton, David

    2017-07-13

    Optical microscopy is the most direct method to probe fluid dynamics at small scales. However, contrast between fluid phases vanishes at ∼10 nm lengthscales, limiting direct optical interrogation to larger systems. Here, we present a method for direct, high-contrast and label-free visualization of fluid dynamics in sub-10 nm channels, and apply this method to study capillary filling dynamics at this scale. The direct visualization of confined fluid dynamics in 8-nm high channels is achieved with a conventional bright-field optical microscope by inserting a layer of a high-refractive-index material, silicon nitride (Si3N4), between the substrate and the nanochannel, and the height of which is accurately controlled down to a few nanometers by a SiO2 spacer layer. The Si3N4 layer exhibits a strong Fabry-Perot resonance in reflection, providing a sharp contrast between ultrathin liquid and gas phases. In addition, the Si3N4 layer enables robust anodic bonding without nanochannel collapse. With this method, we demonstrate the validity of the classical Lucas-Washburn equation for capillary filling in the sub-10 nm regime, in contrast to the previous studies, for both polar and nonpolar liquids, and for aqueous salt solutions.

  5. 78 FR 66982 - Santa Clara Pueblo Disaster #NM-00039

    Science.gov (United States)

    2013-11-07

    ... ADMINISTRATION Santa Clara Pueblo Disaster NM-00039 AGENCY: U.S. Small Business Administration. ACTION: Notice... for the Santa Clara Pueblo (FEMA- 4151-DR), dated 10/29/2013. Incident: Severe Storms and Flooding... disaster: Primary Areas: Santa Clara Pueblo. The Interest Rates are: Percent For Physical Damage:...

  6. EST Table: NM_001046937 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001046937 LOC732938 10/09/29 100 %/220 aa ref|NP_001040402.1| preimplantation pr...otein [Bombyx mori] gb|ABF51322.1| preimplantation protein [Bombyx mori] 10/09/13 79 %/222 aa FBpp0234606|Dv

  7. 77 FR 41874 - New Mexico Disaster #NM-00025

    Science.gov (United States)

    2012-07-16

    ... ADMINISTRATION New Mexico Disaster NM-00025 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of New Mexico dated 07/09/2012. Incident: Little Bear Fire. Incident Period: 06/04/2012 and continuing. Effective Date:...

  8. 77 FR 47907 - New Mexico Disaster #NM-00025

    Science.gov (United States)

    2012-08-10

    ... ADMINISTRATION New Mexico Disaster NM-00025 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is an amendment of the Administrative declaration of a disaster for the State of NEW MEXICO, dated 07/09/2012. Incident: Little Bear Fire. Incident Period: 06/04/2012 through 07/30/2012....

  9. EST Table: NM_001043366 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001043366 Sap-r 10/09/29 98 %/961 aa ref|NP_001036831.1| saposin-related [Bombyx... gnl|Amel|GB16561-PA 10/09/10 42 %/808 aa gi|91077504|ref|XP_966852.1| PREDICTED: similar to saposin isoform 1 [Tribolium castaneum] FS791050 ...

  10. EST Table: NM_001043377 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available moting Protein [Bombyx mori] 10/09/13 30 %/136 aa FBpp0237404|DvirGJ22987-PA 10/08/...NM_001043377 Pp 10/09/29 92 %/154 aa ref|NP_001036842.1| promoting protein [Bombyx mori] dbj|BAA89306.1| Pro

  11. EST Table: NM_001046656 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001046656 LOC692809 11/12/09 10/09/29 80 %/293 aa ref|NP_001040121.1| endothelial-monocyte... activating polypeptide II [Bombyx mori] gb|ABD36113.1| endothelial-monocyte activating polypeptid

  12. A novel double patterning approach for 30nm dense holes

    Science.gov (United States)

    Hsu, Dennis Shu-Hao; Wang, Walter; Hsieh, Wei-Hsien; Huang, Chun-Yen; Wu, Wen-Bin; Shih, Chiang-Lin; Shih, Steven

    2011-04-01

    Double Patterning Technology (DPT) was commonly accepted as the major workhorse beyond water immersion lithography for sub-38nm half-pitch line patterning before the EUV production. For dense hole patterning, classical DPT employs self-aligned spacer deposition and uses the intersection of horizontal and vertical lines to define the desired hole patterns. However, the increase in manufacturing cost and process complexity is tremendous. Several innovative approaches have been proposed and experimented to address the manufacturing and technical challenges. A novel process of double patterned pillars combined image reverse will be proposed for the realization of low cost dense holes in 30nm node DRAM. The nature of pillar formation lithography provides much better optical contrast compared to the counterpart hole patterning with similar CD requirements. By the utilization of a reliable freezing process, double patterned pillars can be readily implemented. A novel image reverse process at the last stage defines the hole patterns with high fidelity. In this paper, several freezing processes for the construction of the double patterned pillars were tested and compared, and 30nm double patterning pillars were demonstrated successfully. A variety of different image reverse processes will be investigated and discussed for their pros and cons. An economic approach with the optimized lithography performance will be proposed for the application of 30nm DRAM node.

  13. EST Table: NM_001172720 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001172720 cact 10/09/29 95 %/326 aa ref|NP_001166191.1| cactus [Bombyx mori] dbj...10/09/10 39 %/197 aa gnl|Amel|GB10655-PA 10/09/10 35 %/291 aa gi|270016252|gb|EFA12698.1| cactus [Tribolium castaneum] AV404402 ...

  14. EST Table: NM_001047011 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001047011 LOC733016 10/09/29 100 %/184 aa ref|NP_001040476.1| muscular protein 2...0 [Bombyx mori] gb|ABF51467.1| muscular protein 20 [Bombyx mori] 10/09/13 77 %/184 aa FBpp0235584|DvirGJ2116

  15. 77 FR 62481 - Radio Broadcasting Services; Crownpoint, NM

    Science.gov (United States)

    2012-10-15

    ... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Crownpoint, NM AGENCY: Federal Communications....415 and 1.420. List of Subjects in 47 CFR Part 73 Radio, Radio broadcasting. Federal...

  16. Conjugated 12 nm long oligomers as molecular wires in nanoelectronics

    DEFF Research Database (Denmark)

    Søndergaard, Roar; Strobel, Sebastian; Bundgaard, Eva

    2009-01-01

    We demonstrate a generic synthetic approach to oligophenylenevinylene (OPV) derivative molecules with a molecular length of up to 12 nm and a relatively free choice of end group that can attach to different electrodes such as metallic gold or potentially transition metal oxide semiconductors. OPVs...

  17. EST Table: NM_001046698 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001046698 LOC692853 10/09/29 93 %/269 aa ref|NP_001040163.1| ischemia/reperfusio...n inducible protein [Bombyx mori] gb|ABD36179.1| ischemia/reperfusion inducible protein [Bombyx mori] 10/09/

  18. Trends and challenges in VLSI technology scaling towards 100 nm

    NARCIS (Netherlands)

    Rusu, S.; Sachdev, M.; Svensson, C.; Nauta, Bram

    Summary form only given. Moore's Law drives VLSI technology to continuous increases in transistor densities and higher clock frequencies. This tutorial will review the trends in VLSI technology scaling in the last few years and discuss the challenges facing process and circuit engineers in the 100nm

  19. Extreme magnetic anisotropy and multiple superconducting transition signatures in a [Nb(23 nm)/Ni(5 nm)] 5 multilayer

    Science.gov (United States)

    De Long, L. E.; Kryukov, S. A.; Joshi, Amish G.; Xu, Wentao; Bosomtwi, A.; Kirby, B. J.; Fitzsimmons, M. R.

    2008-04-01

    We have applied polarized neutron reflectometry, and novel SQUID and vibrating reed magnetometry to probe a [Nb(23 nm)/Ni(5 nm)]5 multilayer (ML) whose superconducting state magnetic anisotropy is dominated by confined (in-plane) supercurrents in DC magnetic fields, H, applied nearly parallel to the ML plane. The upper critical field exhibits abrupt shifts (0.1-0.6 K) in near-parallel fields, but is field-independent for μ0H < 0.8 T when the ML is exactly aligned with the DC field, indicating suppression of orbital pairbreaking and the possible presence of unconventional superconducting pairing states.

  20. Direct writing of 150 nm gratings and squares on ZnO crystal in water by using 800 nm femtosecond laser.

    Science.gov (United States)

    Liu, Jukun; Jia, Tianqing; Zhou, Kan; Feng, Donghai; Zhang, Shian; Zhang, Hongxin; Jia, Xin; Sun, Zhenrong; Qiu, Jianrong

    2014-12-29

    We present a controllable fabrication of nanogratings and nanosquares on the surface of ZnO crystal in water based on femtosecond laser-induced periodic surface structures (LIPSS). The formation of nanogrooves depends on both laser fluence and writing speed. A single groove with width less than 40 nm and double grooves with distance of 150 nm have been produced by manipulating 800 nm femtosecond laser fluence. Nanogratings with period of 150 nm, 300 nm and 1000 nm, and nanosquares with dimensions of 150 × 150 nm2 were fabricated by using this direct femtosecond laser writing technique.

  1. Cryogenic Lifetime Studies of 130 nm and 65 nm CMOS Technologies for High-Energy Physics Experiments

    Energy Technology Data Exchange (ETDEWEB)

    Hoff, James R. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Deptuch, G. W. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Wu, Guoying [Southern Methodist Univ., Dallas, TX (United States); Gui, Ping [Southern Methodist Univ., Dallas, TX (United States)

    2015-06-04

    The Long Baseline Neutrino Facility intends to use unprecedented volumes of liquid argon to fill a time projection chamber in an underground facility. Research is under way to place the electronics inside the cryostat. For reasons of efficiency and economics, the lifetimes of these circuits must be well in excess of 20 years. The principle mechanism for lifetime degradation of MOSFET devices and circuits operating at cryogenic temperatures is hot carrier degradation. Choosing a process technology that is, as much as possible, immune to such degradation and developing design techniques to avoid exposure to such damage are the goals. This, then, requires careful investigation and a basic understanding of the mechanisms that underlie hot carrier degradation and the secondary effects they cause in circuits. In this work, commercially available 130 nm and 65 nm nMOS transistors operating at cryogenic temperatures are investigated. Our results show that both technologies achieve the lifetimes required by the experiment. Minimal design changes are necessary in the case of the 130 nm process and no changes whatsoever are necessary for the 65 nm process.

  2. Study of drain-extended NMOS under electrostatic discharge stress in 28 nm and 40 nm CMOS process

    Science.gov (United States)

    Wang, Weihuai; Jin, Hao; Dong, Shurong; Zhong, Lei; Han, Yan

    2016-02-01

    Researches on the electrostatic discharge (ESD) performance of drain-extended NMOS (DeNMOS) under the state-of-the-art 28 nm and 40 nm bulk CMOS process are performed in this paper. Three distinguishing phases of avalanche breakdown stage, depletion region push-out stage and parasitic NPN turn on stage of the gate-grounded DeNMOS (GG-DeNMOS) fabricated under 28 nm CMOS process measured with transmission line pulsing (TLP) test are analyzed through TCAD simulations and tape-out silicon verification detailedly. Damage mechanisms and failure spots of GG-DeNMOS under both CMOS processes are thermal breakdown of drain junction. Improvements based on the basic structure adjustments can increase the GG-DeNMOS robustness from original 2.87 mA/μm to the highest 5.41 mA/μm. Under 40 nm process, parameter adjustments based on the basic structure have no significant benefits on the robustness improvements. By inserting P+ segments in the N+ implantation of drain or an entire P+ strip between the N+ implantation of drain and polysilicon gate to form the typical DeMOS-SCR (silicon-controlled rectifier) structure, the ESD robustness can be enhanced from 1.83 mA/μm to 8.79 mA/μm and 29.78 mA/μm, respectively.

  3. Design and simulation of 1310 nm and 1480 nm single-mode photonic crystal fiber Raman lasers.

    Science.gov (United States)

    Varshney, S K; Sasaki, K; Saitoh, K; Koshiba, M

    2008-01-21

    We have numerically investigated the Raman lasing characteristics of a highly nonlinear photonic crystal fiber (HNPCF). HNPCF Raman lasers are designed to deliver outputs at 1.3 microm and 1.48 microm wavelengths through three and six cascades of Raman Stokes cavities when the pumps of 1117 nm and 1064 nm are injected into HNPCF module, respectively. A quantum efficiency of approximately 47% was achieved in a short length of HNPCF for 1.3 microm lasing wavelength. The HNPCF design is modified further to operate in single-mode fashion keeping intact its Raman lasing characteristics. The modified HNPCF design consists of two air-hole rings where the higher-order modes in the central core are suppressed by enhancing their leakage losses drastically, thus ceasing their propagation in the short length of HNPCF. On the other hand, the fundamental mode is well confined to the central core region, unaffecting its lasing performances. Further, the lasing characteristics of HNPCF at 1480 nm are compared with conventional highly nonlinear fiber Raman laser operating at 1480 nm. It is found that one can reduce the fiber length by five times in case of HNPCF with nearly similar conversion efficiency.

  4. Pattern generation requirements for mask making beyond 130 nm

    Science.gov (United States)

    Abboud, Frank E.; Gesley, Mark A.; Maldonado, Juan R.

    1998-06-01

    It is commonly accepted in the semiconductor industry that optical lithography will be the most cost-effective solution for 150 nm and 130 nm device generations. Some selected layers at the 130 nm device generation may be produced using electron-beam direct-write or x-ray during the development phase. However, for the production phase, it is expected that 193 nm optical lithography with reticle enhancement techniques such as optical proximity correction (OPC) and phase shift masks (PSM) will be the technology of choice. What about post 193 nm. The range of solutions is more diverse and a clear winner has not yet emerged. The topic, however, is becoming more visible and has taken a prominent place in technical conferences in the past year. The five leading potential alternatives to optical lithography are proximity x-ray, e-beam projection (EBP), extended UV (EUV), ion projection lithography (IPL), and e-beam direct write. The search for the right answer will most likely continue for a few years, and possibly more than one alternative will emerge as an effective solution at and below 100 nm. All of the alternatives, with the exception of e-beam direct write, have one thing in common, the mask. Except for proximity x- ray, all solutions at present envision a 4x reduction of the mask-to-wafer image plane. Instead of chrome-coated quartz, a silicon wafer substrate is used. Aside from patterning, mask fabrication varies depending on the lithography absorbing substrate, and EUV requires a reflective multilayer stack. Most key lithography requirements needed to pattern the imaging layer are common to all of the candidates, at least for the reduction methods. For x-ray lithography, the requirements are significantly more stringent but at a smaller field. This paper will consolidate the requirements of the various types of masks from a pattern generation point of view and will focus on the pattern generation tool requirements to meet those mask requirements. In addition, it

  5. Lasering in a Waveguide with Scatterers in Diameter 20 nm

    Institute of Scientific and Technical Information of China (English)

    LIU Chun-Xu; LIU Jun-Ye; ZHANG Jia-Hua; DOU Kai

    2004-01-01

    We report random lasing achieved in a MEH-PPV/glass waveguide with the TiO2 scatterers in diameter 20nm that is significantly smaller than submicrometre of TiO2 scatterers in the films or suspensions previously reported on random lasing. The spectral lines are dramatically narrowed by almost two orders of magnitude compared with those excited by a xenon lamp. The amplified spontaneous emission is identified as the dominant mechanism in our system. Light localization might be achieved in a broad class of random materials based on the features of the mean free path l* = 5.4 × 105 nm, kl* > 1 and the Thouless number 6.73 × 10-5 with k being the wave number.

  6. Magnetic Behavior of Surface Nanostructured 50-nm Nickel Thin Films

    Directory of Open Access Journals (Sweden)

    Kumar Prashant

    2010-01-01

    Full Text Available Abstract Thermally evaporated 50-nm nickel thin films coated on borosilicate glass substrates were nanostructured by excimer laser (0.5 J/cm2, single shot, DC electric field (up to 2 kV/cm and trench-template assisted technique. Nanoparticle arrays (anisotropic growth features have been observed to form in the direction of electric field for DC electric field treatment case and ruptured thin film (isotropic growth features growth for excimer laser treatment case. For trench-template assisted technique; nanowires (70–150 nm diameters have grown along the length of trench template. Coercive field and saturation magnetization are observed to be strongly dependent on nanostructuring techniques.

  7. EST Table: NM_001173375 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001173375 EcR 10/09/29 89 %/591 aa ref|NP_001166846.1| ecdysone receptor isoform... B1 [Bombyx mori] sp|P49881.1|ECR_BOMMO RecName: Full=Ecdysone receptor; AltName: Full=Ecdysteroid receptor;... AltName: Full=20-hydroxy-ecdysone receptor; Short=20E receptor; AltName: Full=EcRH; AltName: Full=Nuclear r...eceptor subfamily 1 group H member 1 gb|AAA87341.1| 20-hydroxy-ecdysone receptor ...57 %/420 aa gi|270014267|gb|EFA10715.1| ecdysone receptor isoform B [Tribolium castaneum] NM_001043866 ...

  8. A deep-UV optical frequency comb at 205 nm.

    Science.gov (United States)

    Peters, E; Diddams, S A; Fendel, P; Reinhardt, S; Hänsch, T W; Udem, Th

    2009-05-25

    By frequency quadrupling a picosecond pulse train from a Ti:sapphire laser at 820 nm we generate a frequency comb at 205 nm with nearly bandwidth-limited pulses. The nonlinear frequency conversion is accomplished by two successive frequency doubling stages that take place in resonant cavities that are matched to the pulse repetition rate of 82 MHz. This allows for an overall efficiency of 4.5 % and produces an output power of up to 70 mW for a few minutes and 25 mW with continuous operation for hours. Such a deep UV frequency comb may be employed for direct frequency comb spectroscopy in cases where it is less efficient to convert to these short wavelengths with continuous wave lasers.

  9. Induced-dichroism-excited atomic line filter at 532 nm.

    Science.gov (United States)

    Gayen, S K; Billmers, R I; Contarino, V M; Squicciarini, M F; Scharpf, W J; Yang, G; Herczfeld, P R; Allocca, D M

    1995-06-15

    The operation of a narrow-linewidth optical f ilter based on the 4P((1/2)) ? 8S((1/2)) excited-state transition in potassium vapor is reported. The 4P((1/2)) state is excited by a circularly polarized, 769.9-nm, 10-ns pulse from a dye laser. A linearly polarized, time-sequenced, and spatially overlapped probe pulse at 532.33 nm completes the transition to the 8S((1/2)) state. The peak filter transmission is ~40% with a bandwidth of less than 4 GHz. Corroborative experimental results suggest that the rotation of probe-pulse polarization by an induced circular birefringence is the dominant mechanism behind the filter operation.

  10. Internal defect localization in 980 nm ridge waveguide lasers

    Science.gov (United States)

    Díaz, L.; Eichler, H. J.; Weich, K.; Klehr, A.; Zeimer, U.

    2006-04-01

    High power lasers emitting at 980 nm are essential for pumping sources of erbium-doped fiber amplifiers (EDFAs). These are used in longer distance telecommunications. Stability and reliability of the modules are two key characteristics. The present paper investigates 'sudden random failures' of double quantum-well 980 nm high power ridge waveguide lasers implemented in EDFAs. For the inspection of the external and internal status of the device we used optical spectrum modulation experiments, electroluminescence measurements, scanning electron microscopy and cathodoluminescence investigations. The localization of internal defects is the main point of this work. Two different 'sudden random failures' were found: catastrophical optical mirror damage (COMD) and internal dark line defect (DLD) formation.

  11. Sub-5 nm, globally aligned graphene nanoribbons on Ge(001)

    Energy Technology Data Exchange (ETDEWEB)

    Kiraly, Brian [Center for Nanoscale Materials, Argonne National Laboratory, 9700 South Cass Avenue, Building 440, Argonne, Illinois 60439, USA; Department of Materials Science and Engineering, Northwestern University, 2220 Campus Drive, Evanston, Illinois 60208, USA; Mannix, Andrew J. [Center for Nanoscale Materials, Argonne National Laboratory, 9700 South Cass Avenue, Building 440, Argonne, Illinois 60439, USA; Department of Materials Science and Engineering, Northwestern University, 2220 Campus Drive, Evanston, Illinois 60208, USA; Jacobberger, Robert M. [Department of Materials Science and Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA; Fisher, Brandon L. [Center for Nanoscale Materials, Argonne National Laboratory, 9700 South Cass Avenue, Building 440, Argonne, Illinois 60439, USA; Arnold, Michael S. [Department of Materials Science and Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA; Hersam, Mark C. [Department of Materials Science and Engineering, Northwestern University, 2220 Campus Drive, Evanston, Illinois 60208, USA; Department of Chemistry, Northwestern University, 2220 Campus Drive, Evanston, Illinois 60208, USA; Guisinger, Nathan P. [Center for Nanoscale Materials, Argonne National Laboratory, 9700 South Cass Avenue, Building 440, Argonne, Illinois 60439, USA

    2016-05-23

    Graphene nanoribbons (GNRs) hold great promise for future electronics because of their edge and width dependent electronic bandgaps and exceptional transport properties. While significant progress toward such devices has been made, the field has been limited by difficulties achieving narrow widths, global alignment, and atomically pristine GNR edges on technologically relevant substrates. A recent advance has challenged these limits by using Ge(001) substrates to direct the bottom-up growth of GNRs with nearly pristine armchair edges and widths near ~10 nm via atmospheric pressure chemical vapor deposition. In this work, we extend the growth of GNRs on Ge(001) to ultra-high vacuum conditions and realize GNRs narrower than 5 nm. Armchair graphene nanoribbons directed along the Ge <110> surface directions are achieved with excellent width control and relatively large bandgaps. The bandgap magnitude and electronic uniformity make these new materials excellent candidates for future developments in nanoelectronics.

  12. A new DFM flow for sub-100nm standard cells

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    DFM (Design-For-Manufacturability) method, which aims to improve manufacturability of ICs through specific design considerations, is becoming important nowadays. In particular, standard cells now should be designed by DFM method. This paper reports a new DFM flow for sub-100 nm standard cell design with a group of technologies for process modeling, manufacturability simulation and trial RETs.Based on this flow, a set of DFM-friendly 90m standard cells were designed.

  13. EST Table: NM_001114999 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001114999 Hepa 10/09/29 96 %/515 aa ref|NP_001108471.1| heparanase-like protein ...[Bombyx mori] dbj|BAB85191.1| heparanase-like protein [Bombyx mori] dbj|BAC10612.1| heparanase-like protein ...473 aa gi|189236064|ref|XP_971018.2| PREDICTED: similar to heparanase-like protein [Tribolium castaneum] FS913340 ...

  14. Numerical Simulation of Spectral Response for 650 nm Silicon Photodetector

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The theoretical spectral response formula of the N+-N-I-P+ silicon photodetector with high/low emission junction is given. At the same time, considering the process requirements, the optimum structure parameters of silicon photodetector are obtained by numerical calculation and simulation. Under the condition of these optimum structure parameters, the responsivity of the silicon photodetector will be 0.48A/W at 650nm.

  15. EST Table: NM_001044218 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001044218 glv2 10/09/29 100 %/173 aa ref|NP_001037683.1| gloverin 2 [Bombyx mori] dbj|BAE53372.1| antibac...terial peptide [Bombyx mori] dbj|BAF51564.1| gloverin2 [Bombyx mori] 10/09/13 n.h 10/08/29 n.h 10/09/10 n.h 10/09/10 n.h 10/09/10 n.h FS917189 ...

  16. EST Table: NM_001044219 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001044219 glv4 10/09/29 100 %/171 aa ref|NP_001037684.1| gloverin 4 [Bombyx mori] dbj|BAE53373.1| antibac...terial peptide [Bombyx mori] dbj|BAF63528.1| gloverin4 [Bombyx mori] 10/09/13 n.h 10/08/29 n.h 10/09/10 n.h 10/09/10 n.h 10/09/10 n.h FS798027 ...

  17. EST Table: NM_001043467 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001043467 glv4-like 10/09/29 100 %/171 aa ref|NP_001036932.1| gloverin 4-like [B...ombyx mori] dbj|BAD51476.1| gloverin-like protein 4 [Bombyx mori] 10/09/13 n.h 10/08/29 n.h 10/09/10 n.h 10/09/10 n.h 10/09/10 n.h FS798027 ...

  18. EST Table: NM_001046863 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001046863 Surf1 10/09/29 100 %/294 aa ref|NP_001040328.1| surfeit 1 isoform 1 [B...ombyx mori] gb|ABD36358.1| surfeit protein isoform 1 [Bombyx mori] 10/09/13 45 %/250 aa FBpp0162190|DmojGI12...|XP_972868.1| PREDICTED: similar to surfeit locus protein [Tribolium castaneum] FS918885 ...

  19. EST Table: NM_001173359 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001173359 Surf1 10/09/29 100 %/228 aa ref|NP_001166830.1| surfeit 1 isoform 2 [B...ombyx mori] gb|ABD36359.1| surfeit protein isoform 2 [Bombyx mori] 10/09/13 35 %/216 aa FBpp0179156|DperGL15...|XP_972868.1| PREDICTED: similar to surfeit locus protein [Tribolium castaneum] FS918885 ...

  20. EST Table: NM_001043866 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available )|GO:0043565(sequence-specific DNA binding) 10/09/29 89 %/588 aa ref|NP_001037331.2| ecdysone receptor isofo...rm B2 [Bombyx mori] gb|AAA87340.1| 20-hydroxy-ecdysone receptor [Bombyx mori] 10/09/13 61 %/442 aa FBpp01170...0014267|gb|EFA10715.1| ecdysone receptor isoform B [Tribolium castaneum] NM_001043866 ...

  1. EST Table: NM_001046659 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001046659 LOC692812 10/09/29 88 %/413 aa ref|NP_001040124.1| exuperantia [Bombyx... mori] gb|ABD36117.1| exuperantia [Bombyx mori] 10/09/13 34 %/368 aa FBpp0233182|Dvirexu-PA 10/08/29 n.h 10/...|GB19360-PA 10/09/10 44 %/375 aa gi|91085815|ref|XP_974770.1| PREDICTED: similar to exuperantia [Tribolium castaneum] FS919769 ...

  2. A tunable, single frequency, fiber ring at 1053 nm

    Energy Technology Data Exchange (ETDEWEB)

    Wilcox, R.B.

    1997-02-21

    This laser is a tunable source designed for applications where a shorter pulse will be chopped from a long Q-switched pulse by electrooptic modulators, then amplified in Nd:phosphate glass. The laser employs ytterbium-doped silica fiber as the gain medium, pumped by a laser diode at 980nm. Gain in Yb:silica is distributed over an 90nm range, making it suitable for operation at many wavelengths. Our previous experiments with this medium demonstrated oscillation over a 50nm wide band. In addition, pumping at 980nm allows the use of stable pump diodes used in erbium-doped fiber amplifiers (EDFA`s). We designed the laser to take advantage of this wideband gain medium, and yet operate on a single cavity mode. A circulator causes unidirectional operation, and allows use of a fiber grating in reflection. This grating has a 0.2 Angstrom bandwidth, and defines the coarse tuning of the laser. It is piezoelectrically stretch tuned to the desired wavelength band. A single mode of the cavity is selected by a piezoelectrically tuned fiber grating Fabry-Perot etalon with 64MHz bandwidth. The laser is Q-switched by a bulk acousto-optic device at lkhz reprate. The loss is controlled to allow the oscillator to lase close to threshold for 500{micro}s before the Q-switch is turned off completely, creating a pulse. This ``pre-lasing`` stabilizes the single mode, since Q-switch pulse builds up from the prelase level. To prevent mode hopping during long term operation, cavity length is feedback controlled. Another piezoelectric device stretches a fiber in the cavity according to an error signal derived from the output optical signal. Due to the long, high loss cavity, the Q-switched pulse is about 3OOns long. The central part of this pulse will be gated by an electrooptic modulator to produce a 30ns square pulse, used for further amplification and modulation.

  3. High Harmonic Inverse Free Electron Laser Interaction at 800 nm

    Energy Technology Data Exchange (ETDEWEB)

    Sears, C

    2005-03-08

    We demonstrate for the first time an inverse free electron laser (IFEL) operating at 800 nm and observe multiple resonances of the IFEL interaction. The IFEL is tested at half its fundamental resonance electron energy and scanned through multiple harmonics by adjusting the undulator field strength. We obtain a peak modulation of {approx}50 keV FWHM and observe the 4th through 6th harmonics of the IFEL resonance.

  4. EST Table: NM_001043365 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001043365 trh 10/09/29 91 %/849 aa ref|NP_001036830.1| trachealess [Bombyx mori] dbj|BAA22946.1| Bm trach...GB30139-PA 10/09/10 57 %/875 aa gi|189241974|ref|XP_967112.2| PREDICTED: similar to Bm trachealess [Tribolium castaneum] FS923495 ... ...ealess [Bombyx mori] 10/09/13 50 %/973 aa FBpp0110175|trh-PB 10/08/29 low homology

  5. EST Table: NM_001046972 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001046972 LOC732975 10/09/29 100 %/182 aa ref|NP_001040437.1| muscular protein 2...0 [Bombyx mori] gb|ABF51386.1| muscular protein 20 [Bombyx mori] 10/09/13 61 %/173 aa FBpp0235584|DvirGJ2116...aa gi|91077564|ref|XP_972465.1| PREDICTED: similar to muscular protein 20 [Tribolium castaneum] FS765856 ...

  6. Photodissociation cross section of ClOOCl at 330 nm.

    Science.gov (United States)

    Jin, Bing; Chen, I-Cheng; Huang, Wen-Tsung; Lien, Chien-Yu; Guchhait, Nikhil; Lin, Jim J

    2010-04-15

    The photolysis rate of ClOOCl is crucial in the catalytic destruction of polar stratospheric ozone. In this work, we determined the photodissociation cross section of ClOOCl at 330 nm with a molecular beam and with mass-resolved detection. The photodissociation cross section is the product of the absorption cross section and the dissociation quantum yield. We formed an effusive molecular beam of ClOOCl at a nozzle temperature of 200 or 250 K and determined its photodissociation probability by measuring the decrease of the ClOOCl intensity upon laser irradiation. By comparing with a reference molecule (Cl(2)), of which the absorption cross section and dissociation quantum yield are well-known, we determined the absolute photodissociation cross section of ClOOCl at 330 nm to be (2.31 +/- 0.11) x 10(-19) cm(2) at 200 K and (2.47 +/- 0.12) x 10(-19) cm(2) at 250 K. Impurity interference has been a well-recognized problem in conventional spectroscopic studies of ClOOCl; our mass-resolved measurement directly overcomes such a problem. This measurement of the ClOOCl photolysis cross section at 330 nm is particularly useful in constraining its atmospheric photolysis rate, which in the polar stratosphere peaks near this wavelength.

  7. Advanced CMOS device technologies for 45 nm node and below

    Directory of Open Access Journals (Sweden)

    A. Veloso, T. Hoffmann, A. Lauwers, H. Yu, S. Severi, E. Augendre, S. Kubicek, P. Verheyen, N. Collaert, P. Absil, M. Jurczak and S. Biesemans

    2007-01-01

    Full Text Available We review and discuss the latest developments and technology options for 45 nm node and below, with scaled planar bulk MOSFETs and MuGFETs as emerging devices. One of the main metal gate (MG candidates for scaled CMOS technologies are fully silicided (FUSI gates. In this work, by means of a selective and controlled poly etch-back integration process, dual work-function Ni-based FUSI/HfSiON CMOS circuits with record ring oscillator performance (high-VT are reported (17 ps at VDD=1.1 V and 20 pA/μm Ioff, meeting the ITRS 45 nm node requirement for low-power (LP CMOS. Compatibility of FUSI and other MG with known stress boosters like stressed CESL (contact-etch-stop-layer with high intrinsic stress or embedded SiGe in the pMOS S/D regions is validated. To obtain MuGFET devices that are competitive, as compared to conventional planar bulk devices, and that meet the stringent drive and leakage current requirements for the 32 nm node and beyond, higher channel mobilities are required. Results obtained by several strain engineering methods are presented here.

  8. Remote-sensing vibrometry at 1550 nm wavelength

    Science.gov (United States)

    Dräbenstedt, A.; Sauer, J.; Rembe, C.

    2012-06-01

    Laser-Doppler vibrometry (LDV) is a proven technique for vibration analysis of mechanical structures. A wavelength of 633 nm is usually employed because of the availability of the relatively inexpensive Helium-Neon laser source which has a good coherence behavior. However, coherence break-down through the beat of multiple longitudinal modes and the limited detector carrier-to-noise-ratio (CNR) at a measurement laser power of 1 mW have prevented a wide use of LDV in remote sensing applications. Such applications in civil engineering are bridges, towers or wind turbines. The lower photon energy of IR light at 1550 nm wavelength increases the CNR by a factor 2.4. This helps especially in the condition where the carrier power decreases below the FM threshold. We have designed a heterodyne interferometer which allows the shot noise limited detection at 1550 nm wavelength close to the theoretical possible CNR. We present calculations of the fundamental noise contributions in interferometric light detection for a comparison of the achievable CNR between common HeNe vibrometers and IR vibrometers. The calculations are backed by measurements that show the devices working close to the theoretical limits. The achievable noise level of the demodulated velocity signal is shown in dependence from the standoff distance. Our novel heterodyne interferometer has been transferred to the Polytec product RSV-150. An application example of this new sensor will be demonstrated.

  9. Advances in 193 nm excimer lasers for mass spectrometry applications

    Science.gov (United States)

    Delmdahl, Ralph; Esser, Hans-Gerd; Bonati, Guido

    2016-03-01

    Ongoing progress in mass analysis applications such as laser ablation inductively coupled mass spectrometry of solid samples and ultraviolet photoionization mediated sequencing of peptides and proteins is to a large extent driven by ultrashort wavelength excimer lasers at 193 nm. This paper will introduce the latest improvements achieved in the development of compact high repetition rate excimer lasers and elaborate on the impact on mass spectrometry instrumentation. Various performance and lifetime measurements obtained in a long-term endurance test over the course of 18 months will be shown and discussed in view of the laser source requirements of different mass spectrometry tasks. These sampling type applications are served by excimer lasers delivering pulsed 193 nm output of several mJ as well as fast repetition rates which are already approaching one Kilohertz. In order to open up the pathway from the laboratory to broader market industrial use, sufficient component lifetimes and long-term stable performance behavior have to be ensured. The obtained long-term results which will be presented are based on diverse 193 nm excimer laser tube improvements aiming at e.g. optimizing the gas flow dynamics and have extended the operational life the laser tube for the first time over several billion pulses even under high duty-cycle conditions.

  10. 7-nm e-beam resist sensitivity characterization

    Science.gov (United States)

    Zweber, Amy; Toda, Yusuke; Sakamoto, Yoshifumi; Faure, Thomas; Rankin, Jed; Nash, Steven; Kagawa, Masayuki; Fahrenkopf, Michael; Isogawa, Takeshi; Wistrom, Richard

    2016-10-01

    Over time mask makers have been driven to low sensitivity e-beam resist materials to meet lithographic patterning needs. For 7-nm logic node, resolution enhancement techniques continue to evolve bringing more complexity on mask and additional mask builds per layer. As demonstrated in literature, low sensitivity materials are needed for low line edge roughness (LER) but impact write tool through put. In characterizing resist sensitivity for 7-nm, we explore more broadly what advantages and disadvantages moving to lower sensitivity resist materials brings, where LER, critical dimension uniformity, resolution, fogging, image placement, and write time results and trends are presented. In this paper, resist material performance are reported for sensitivities ranging from 20 to 130 μC/cm2 at 50% proximity effect correction, where the exposure will be using a single beam platform. Materials examined include negative tone resist types with chemical amplification and positive tone without chemical amplification focusing on overall trends for 7-nm e-beam resist performance.

  11. The analysis of polarization characteristics on 40nm memory devices

    Science.gov (United States)

    Yoo, Minae; Park, Chanha; You, Taejun; Yang, Hyunjo; Min, Young-Hong; Park, Ki-Yeop; Yim, Donggyu; Park, Sungki

    2009-03-01

    Hyper NA system has been introduced to develop sub-60nm node memory devices. Especially memory industries including DRAM and NAND Flash business have driven much finer technology to improve productivity. Polarization at hyper NA has been well known as important optical technology to enhance imaging performance and also achieve very low k1 process. The source polarization on dense structure has been used as one of the major RET techniques. The process capabilities of various layers under specific illumination and polarization have been explored. In this study, polarization characteristic on 40nm memory device will be analyzed. Especially, TE (Transverse Electric) polarization and linear X-Y polarization on hyper NA ArF system will be compared and investigated. First, IPS (Intensity in Preferred State) value will be measured with PMM (Polarization Metrology Module) to confirm polarization characteristic of each machine before simulation. Next simulation will be done to estimate the CD variation impact of each polarization to different illumination. Third, various line and space pattern of DRAM and Flash device will be analyzed under different polarized condition to see the effect of polarization on CD of actual wafer. Finally, conclusion will be made for this experiment and future work will be discussed. In this paper, the behavior of 40nm node memory devices with two types of polarization is presented and the guidelines for polarization control is discussed based on the patterning performances.

  12. Oxalyl chloride, ClC(O)C(O)Cl: UV/vis spectrum and Cl atom photolysis quantum yields at 193, 248, and 351 nm.

    Science.gov (United States)

    Ghosh, Buddhadeb; Papanastasiou, Dimitrios K; Burkholder, James B

    2012-10-28

    .05 ± 0.24. As part of this work, rate coefficients for the thermal decomposition of ClCO were measured between 253 and 298 K at total pressures between 13 and 128 Torr (He and N(2) bath gases). The N(2) bath gas results were combined with the data reported in Nicovich et al. [J. Chem. Phys. 92, 3539-3544 (1990)] to yield k(4)(T, N(2)) = (4.7 ± 0.7) × 10(-10) exp [-(2987 ± 16)/T] cm(3) molecule(-1) s(-1), while the He bath gas data fit yielded k(4)(T, He) = (2.3 ± 2.1) × 10(-10) exp [-(2886 ± 218)/T] cm(3) molecule(-1) s(-1). The quoted uncertainties are at the 2σ level from the precision of the fit. In addition, the room temperature rate coefficient for the Cl + ClNO reaction was measured in this work to be (1.03 ± 0.10) × 10(-10) cm(3) molecule(-1) s(-1).

  13. Study on CW Nd:YAG infrared laser at 1319 nm

    Institute of Scientific and Technical Information of China (English)

    Tao Wang (王涛); Jianquan Yao (姚建铨); Guojun Yu (禹国俊); Peng Wang (王鹏); Xifu Li (李喜福); Yizhong Yu (于意仲)

    2003-01-01

    A continuous wave (CW) Nd:YAG infrared laser at 1319 nm is reported in this paper. The energy level of 1319-nm wave was analyzed. The repression of 1064-nm lasing and enhancement of 1319-nm output power were discussed. Mirror coating and cavity structure were studied and a maximum CW output power of 43W at 1319 nm was achieved in experiments.

  14. Vortex State in Sub-100 nm Magnetic Nanodots.

    Science.gov (United States)

    Roshchin, Igor V.

    2006-03-01

    Magnetism of nanostructured magnets, which size is comparable to or smaller than ferromagnetic domain size, offers a great potential for new physics. Detailed knowledge of magnetization reversal and possible magnetic configurations in magnetic nanostructures is essential for high-density magnetic memory. Many theoretical and experimental studies are focused on a magnetic vortex which in addition to a circular in-plane configuration of spins has a core, - the region with out-of-plane magnetization. We present a quantitative study of the magnetic vortex state and the vortex core in sub-100 nm magnetic dots. Arrays of single-layer and bilayer nanodots covering over 1 cm^2 are fabricated using self-assembled nanopores in anodized alumina. This method allows good control over the dot size and periodicity. Magnetization measurements performed using SQUID, VSM, and MOKE indicate a transition from a vortex to a single domain state for the Fe dots. This transition is studied as a function of the magnetic field and dots size. Micromagnetic and Monte Carlo simulations confirm the experimental observations. Thermal activation and exchange bias strongly affect the vortex nucleation field and have a much weaker effect on the vortex annihilation field. Direct imaging of magnetic moments in sub-100 nm dots is extremely difficult and has not been reported yet. Polarized grazing incidence small angle neutron scattering measurements allow dot imaging in reciprocal space. Quantitative analysis of such measurements performed on 65 nm Fe dots yields the vortex core size of ˜15 nm, in good agreement with the 14 nm obtained from the simulations. This work is done in collaboration with Chang-Peng Li, Zhi-Pan Li, S. Roy, S. K. Sinha, (UCSD), Xavier Batlle (U. Barcelona), R. K. Dumas, Kai Liu, (UC Davis), S. Park, R. Pynn, M. R. Fitzsimmons (LANL), J. Mejia Lopez (Pontificia U. Catolica de Chile), D. Altbir, (U. de Santiago de Chile), A. H. Romero (Cinvestav-Unidad Queretaro), and Ivan K

  15. Inline detection of Chrome degradation on binary 193nm photomasks

    Science.gov (United States)

    Dufaye, Félix; Sippel, Astrid; Wylie, Mark; García-Berríos, Edgardo; Crawford, Charles; Hess, Carl; Sartelli, Luca; Pogliani, Carlo; Miyashita, Hiroyuki; Gough, Stuart; Sundermann, Frank; Brochard, Christophe

    2013-09-01

    193nm binary photomasks are still used in the semiconductor industry for the lithography of some critical layers for the nodes 90nm and 65nm, with high volumes and over long periods. However, these 193nm binary photomasks can be impacted by a phenomenon of chrome oxidation leading to critical dimensions uniformity (CDU) degradation with a pronounced radial signature. If not detected early enough, this CDU degradation may cause defectivity issues and lower yield on wafers. Fortunately, a standard cleaning and repellicle service at the mask shop has been demonstrated as efficient to remove the grown materials and get the photomask CD back on target.Some detection methods have been already described in literature, such as wafer CD intrafield monitoring (ACLV), giving reliable results but also consuming additional SEM time with less precision than direct photomask measurement. In this paper, we propose another approach, by monitoring the CDU directly on the photomask, concurrently with defect inspection for regular requalification to production for wafer fabs. For this study, we focused on a Metal layer in a 90nm technology node. Wafers have been exposed with production conditions and then measured by SEM-CD. Afterwards, this photomask has been measured with a SEM-CD in mask shop and also inspected on a KLA-Tencor X5.2 inspection system, with pixels 125 and 90nm, to evaluate the Intensity based Critical Dimension Uniformity (iCDU) option. iCDU was firstly developed to provide feed-forward CDU maps for scanner intrafield corrections, from arrayed dense structures on memory photomasks. Due to layout complexity and differing feature types, CDU monitoring on logic photomasks used to pose unique challenges.The selection of suitable feature types for CDU monitoring on logic photomasks is no longer an issue, since the transmitted intensity map gives all the needed information, as shown in this paper. In this study, the photomask was heavily degraded after more than 18,000 300

  16. Successful use of 1064 Nm Nd:YAG in conjunction with 2790 Nm YSGG ablative laser for traumatic scarring.

    Science.gov (United States)

    Nijhawan, Rajiv I; Perez, Maritza I

    2014-01-01

    Patients with traumatic scarring often seek both aesthetic and functional improvement and can be a challenge to treat; however, advances in laser and light technologies have helped to treat many of these patients with rather minimally invasive approaches. A nineteen year old girl with Fitzpatrick skin type III skin presented for the evaluation of extensive traumatic scarring involving her right cheek, right chin, and right oral commissure that she sustained after a motor vehicle accident. We report the successful use of the 1064 nm Nd:YAG laser in conjunction with the ablative 2790 nm YSGG laser for the treatment of traumatic scarring in this patient. Our patient noted a notable improvement in the appearance of her traumatic scarring in addition to decrease in contracture of the right oral commissure. The treatment regimen described provides an effective option for clinicians to utilize when treating traumatic scarring and skin textural changes.

  17. Squeezing visible light waves into a 3-nm-thick and 55-nm-long plasmon cavity.

    Science.gov (United States)

    Miyazaki, Hideki T; Kurokawa, Yoichi

    2006-03-10

    We demonstrate controlled squeezing of visible light waves into nanometer-sized optical cavities. The light is perpendicularly confined in a few-nanometer-thick SiO2 film sandwiched between Au claddings in the form of surface plasmon polaritons and exhibits Fabry-Perot resonances in a longitudinal direction. As the thickness of the dielectric core is reduced, the plasmon wavelength becomes shorter; then a smaller cavity is realized. A dispersion relation down to a surface plasmon wavelength of 51 nm for a red light, which is less than 8% of the free-space wavelength, was experimentally observed. Any obvious breakdowns of the macroscopic electromagnetics based on continuous dielectric media were not disclosed for 3-nm-thick cores.

  18. Sub-70 nm resolution tabletop microscopy at 13.8 nm using a compact laser-plasma EUV source.

    Science.gov (United States)

    Wachulak, Przemyslaw W; Bartnik, Andrzej; Fiedorowicz, Henryk

    2010-07-15

    We report the first (to our knowledge) demonstration of a tabletop, extreme UV (EUV) transmission microscope at 13.8 nm wavelength with a spatial (half-pitch) resolution of 69 nm. In the experiment, a compact laser-plasma EUV source based on a gas puff target is applied to illuminate an object. A multilayer ellipsoidal mirror is used to focus quasi-monochromatic EUV radiation onto the object, while a Fresnel zone plate objective forms the image. The experiment and the spatial resolution measurements, based on a knife-edge test, are described. The results might be useful for the realization of a compact high-resolution tabletop imaging systems for actinic defect characterization.

  19. Megahertz FDML Laser with up to 143nm Sweep Range for Ultrahigh Resolution OCT at 1050nm

    CERN Document Server

    Kolb, Jan Philip; Eibl, Mattias; Pfeiffer, Tom; Wieser, Wolfgang; Huber, Robert

    2016-01-01

    We present a new design of a Fourier Domain Mode Locked laser (FDML laser), which provides a new record in sweep range at ~1um center wavelength: At the fundamental sweep rate of 2x417 kHz we reach 143nm bandwidth and 120nm with 4x buffering at 1.67MHz sweep rate. The latter configuration of our system is characterized: The FWHM of the point spread function (PSF) of a mirror is 5.6um (in tissue). Human in vivo retinal imaging is performed with the MHz laser showing more details in vascular structures. Here we could measure an axial resolution of 6.0um by determining the FWHM of specular reflex in the image. Additionally, challenges related to such a high sweep bandwidth such as water absorption are investigated.

  20. Megahertz FDML laser with up to 143nm sweep range for ultrahigh resolution OCT at 1050nm

    Science.gov (United States)

    Kolb, Jan Philip; Klein, Thomas; Eibl, Matthias; Pfeiffer, Tom; Wieser, Wolfgang; Huber, Robert

    2016-03-01

    We present a new design of a Fourier Domain Mode Locked laser (FDML laser), which provides a new record in sweep range at ~1μm center wavelength: At the fundamental sweep rate of 2x417 kHz we reach 143nm bandwidth and 120nm with 4x buffering at 1.67MHz sweep rate. The latter configuration of our system is characterized: The FWHM of the point spread function (PSF) of a mirror is 5.6μm (in tissue). Human in vivo retinal imaging is performed with the MHz laser showing more details in vascular structures. Here we could measure an axial resolution of 6.0μm by determining the FWHM of specular reflex in the image. Additionally, challenges related to such a high sweep bandwidth such as water absorption are investigated.

  1. A reflectivity profilometer for the optical characterisation of grade reflectivity mirrors in the 250 nm - 1100 nm spectral region

    Energy Technology Data Exchange (ETDEWEB)

    Colucci, Alessandro; Nichelatti, Enrico [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dipt. Innovazione

    1998-04-01

    It`s developed the prototype of an instrument that can be used for the optical characterisation of graded reflectivity mirrors at any wavelength in the spectral region from 250 nm to 1100 nm. The instrument utilises a high-pressure Xe arc lamp as light source. Light is spectrally filtered by means of a grating monochromator. The sample is illuminated with an image of the monochromator exit slit. After reflection from the sample, this image is projected onto a 1024-elements charge-coupled device linear array driven by a digital frame board and interfaced with a personal computer. It`s tested the instrument accuracy by comparing measurement results with the corresponding ones obtained by means of a laser scanning technique. Measurement Rms repeatability has been estimated to be approximately of 0.8%. [Italiano] E` stato sviluppato il prototipo di uno strumento per la catatterizzazione ottica di specchi a riflettivita` variabile, operante a qualsiasi lunghezza d`onda nell`intervallo spettrale da 250 nm a 1100 nm. La sorgente dello strumento e` una lampada ad arco allo Xenon ad alta pressione. La luce e` filtrata spettralmente per mezzo di un monocromatore a reticolo. Il campione viene illuminato da un`immagine della fenditura d`uscita del monocromatore. Dopo essere stata riflessa dal campione, questa immagine viene proiettata su un array CCD lineare a 1024 elementi, connesso elettronicamente a una scheda digitale e interfacciato a un personal computer. L`accuratezza dello strumento e` stata verificata confrontando alcune misure con le corrispondenti misure ottenute mediante una tecnica a scansione laser. La ripetibilita` RMS delle misure e` stata stimata essere circa dello 0.8%.

  2. Emission of electrons from rare gas clusters after irradiation with intense VUV pulses of wavelength 100 nm and 32 nm

    Science.gov (United States)

    Ziaja, B.; Laarmann, T.; Wabnitz, H.; Wang, F.; Weckert, E.; Bostedt, C.; Möller, T.

    2009-10-01

    Kinetic Boltzmann equations are used to describe electron emission spectra obtained after irradiation of noble-gas clusters with intense vacuum ultraviolet (VUV) radiation from a free-electron-laser (FEL). The experimental photoelectron spectra give a complementary and more detailed view of nonlinear processes within atoms and clusters in an intense laser field compared to mass spectroscopy data. Results from our model obtained in this study confirm the experimental and theoretical findings on the differing ionization scenarios at longer (100 nm) and shorter (32 nm) VUV radiation wavelengths. At the wavelength of 100 nm the thermoelectronic electron emission dominates the emission spectra. This indicates the plasma formation and the inverse bremsstrahlung (IB) heating of electrons inside the plasma. This effect is clearly visible for xenon (with the fitted temperature of 6-7 eV), and less visible for argon (with the fitted temperature of 2-3 eV). The two-photon-ionization rate for argon that initiates the cluster ionization, is much lower than the single-photoionization rate for xenon. Also, more of the photoelectrons created within an argon cluster are able to leave it, as they are more energetic than those released from a xenon cluster. Therefore, the IB heating of plasma electrons in argon is less efficient than in xenon, as the density of the electrons remaining within the cluster is lower. At a wavelength of 32 nm the dominant ionization mechanism identified from the electron spectra of argon clusters is the direct multistep ionization. The signature of the thermalization of electrons is also observed. However, as the heating of electrons due to the inverse bremsstrahlung process is weak at these radiation wavelengths and pulse fluences, the increase of the electron temperature with the pulse intensity is mainly due to the increasing photoionization rate within the irradiated sample.

  3. Comparison of the photothermal effects of 808nm gold nanorod and indocyanine green solutions using an 805nm diode laser

    Science.gov (United States)

    Hasanjee, Aamr M.; Zhou, Feifan; West, Connor; Silk, Kegan; Doughty, Austin; Bahavar, Cody F.; Chen, Wei R.

    2016-03-01

    Non-invasive laser immunotherapy (NLIT) is a treatment method for metastatic cancer which combines noninvasive laser irradiation with immunologically modified nanostructures to ablate a primary tumor and induce a systemic anti-tumor response. To further expand the development of NLIT, two different photosensitizing agents were compared: gold nanorods (GNR) with an optical absorption peak of 808 nm and indocyanine green (ICG) with an optical absorption peak of ~800 nm. Various concentrations of GNR and ICG solutions were irradiated at different power densities using an 805 nm diode laser, and the temperature of the solutions was monitored during irradiation using a thermal camera. For comparison, dye balls made up of a 1:1 volume ratio of gel solution to GNR or ICG solution were placed in phantom gels and were then irradiated using the 805 nm diode laser to imitate the effect of laser irradiation on in vivo tumors. Non-invasive laser irradiation of GNR solution for 2 minutes resulted in a maximum increase in temperature by 31.8 °C. Additionally, similar irradiation of GNR solution dye ball within phantom gel for 10 minutes resulted in a maximum temperature increase of 8.2 °C. Comparatively, non-invasive laser irradiation of ICG solution for 2 minutes resulted in a maximum increase in temperature by 28.0 °C. Similar irradiation of ICG solution dye ball within phantom gel for 10 minutes yielded a maximum temperature increase of only 3.4 °C. Qualitatively, these studies showed that GNR solutions are more effective photosensitizing agents than ICG solution.

  4. 2 nm continuously tunable 488nm micro-integrated diode-laser-based SHG light source for Raman spectroscopy

    Science.gov (United States)

    Braune, M.; Maiwald, M.; Sumpf, B.; Tränkle, G.

    2016-04-01

    Raman spectroscopy in the visible spectral range is of great interest due to resonant Raman effects. Nevertheless, fluorescence and ambient light can mask the weak Raman lines. Shifted excitation Raman difference spectroscopy is a demonstrated tool to overcome this drawback. To apply this method, a light source with two alternating wavelengths is necessary. The spectral distance between these two wavelengths has to be adapted to the width of the Raman signal. According to the sample under investigation the width of the Raman signal could be in the range of 3 cm-1 - 12 cm-1. In this work, a micro-integrated light source emitting at 488 nm with a continuous wavelength tuning range up to 2 nm (83 cm-1) is presented. The pump source, a DFB laser emitting at 976 nm, and a periodically poled lithium niobate (PPLN) ridge waveguide crystal is used for the second harmonic generation (SHG). Both components are mounted on a μ-Peltier-element for temperature control. Here, a common wavelength tuning of the pump wavelength and the acceptance bandwidth of the SHG crystal via temperature is achieved. With the results the light source is suitable for portable Raman and SERDS experiments with a flexible spectral distance between both excitation wavelengths for SERDS with respect to the sample under investigation.

  5. Writing time estimation of EB mask writer EBM-9000 for hp16nm/logic11nm node generation

    Science.gov (United States)

    Kamikubo, Takashi; Takekoshi, Hidekazu; Ogasawara, Munehiro; Yamada, Hirokazu; Hattori, Kiyoshi

    2014-10-01

    The scaling of semiconductor devices is slowing down because of the difficulty in establishing their functionality at the nano-size level and also because of the limitations in fabrications, mainly the delay of EUV lithography. While multigate devices (FinFET) are currently the main driver for scalability, other types of devices, such as 3D devices, are being realized to relax the scaling of the node. In lithography, double or multiple patterning using ArF immersion scanners is still a realistic solution offered for the hp16nm node fabrication. Other lithography candidates are those called NGL (Next Generation Lithography), such as DSA (Directed-Self-Assembling) or nanoimprint. In such situations, shot count for mask making by electron beam writers will not increase. Except for some layers, it is not increasing as previously predicted. On the other hand, there is another aspect that increases writing time. The exposure dose for mask writing is getting higher to meet tighter specifications of CD uniformity, in other words, reduce LER. To satisfy these requirements, a new electron beam mask writer, EBM-9000, has been developed for hp16nm/logic11nm generation. Electron optical system, which has the immersion lens system, was evolved from EBM-8000 to achieve higher current density of 800A/cm2. In this paper, recent shot count and dose trend are discussed. Also, writing time is estimated for the requirements in EBM-9000.

  6. Mechanistic comparison of pulse laser induced phase separation of particulates from cellulose paper at 213 nm and 532 nm

    Energy Technology Data Exchange (ETDEWEB)

    Arif, S.; Forster, M.; Kautek, W. [University of Vienna, Department of Physical Chemistry, Wien (Austria); Bushuk, S.; Kouzmouk, A.; Tatur, H.; Batishche, S. [National Academy of Sciences of the Republic of Belarus, Institute of Physics, Minsk (Belarus)

    2013-02-15

    The laser-induced phase separation of charcoal particles on additive-free cotton linters cellulose paper was investigated by electron and optical microscopy, colorimetry, and diffuse reflectance FT-IR. The fibre bundles were vaporised in depth of several 10 {mu}m above destruction fluence thresholds using visible 532 nm radiation. This is in contrast to mid-ultraviolet 213 nm radiation, where only the top fibre bundles were modified and partially evaporated. The colorimetric lightness results generally represented the cleaning status, whereas the colorimetric yellowing data represented irreversible chemical and/or photochemical changes. Charcoal-contaminated paper treated with visible and mid-ultraviolet radiation exhibited yellowing, whereas uncontaminated did not. This suggests that the electron-rich plasma generated by the evaporation of the particles heats the adjacent substrate and also excludes oxygen. Mid-ultraviolet, in contrast to visible radiation, shows particle removal always accompanied by paper destruction. IR spectroscopy results suggest cross-linking by ether bonds near the destruction threshold, but do not prove the formation of oxidation products and double bonds as the basis of the yellowing. A ''cleaning window'' between the cleaning threshold (0.1 J/cm{sup 2}) and the paper destruction threshold (2.9 J/cm{sup 2}) with a pulse number of 2 is provided by visible 532 nm laser treatment. (orig.)

  7. Report on EUROMET.RI(I)-K1 and EUROMET.RI(I)-K4 (EUROMET project no. 813): Comparison of air kerma and absorbed dose to water measurements of {sup 60}Co radiation beams for radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Csete, I. [National Office of Measures (OMH) - pilot laboratory and corresponding author (Hungary); Leiton, A.G. [Research Centre for Energy, Environment and Technology (CMRI-CIEMAT) (Spain); Sochor, V. [Czech Metrology Institute (CMI) (Czech Republic); Lapenas, A. [Latvian National Metrology Center (LNMC-RMTC) (Latvia); Grindborg, J.E. [Swedish Radiation Protection Authority (SSI) (Sweden); Jokelainen, I. [Radiation and Nuclear Safety Authority (STUK) (Finland); Bjerke, H. [Norwegian Radiation Protection Authority (NRPA) (Norway); Dobrovodsky, J. [Slovak Institute of Metrology (SMU) (Slovakia); Megzifene, A. [International Atomic Energy Agency, IAEA, Vienna (Austria); Hourdakis, C.J. [Hellenic Atomic Energy Committee (HAEC-HIRCL) (Greece); Ivanov, R. [National Centre of Metrology (NCM) (Bulgaria); Vekic, B. [Rudjer Boskovic Institute (IRB) (Croatia); Kokocinski, J. [Central Office of Measures (GUM) (Poland); Cardoso, J. [Institute for Nuclear Technology (ITN-LMRIR) (Portugal); Buermann, L. [Physikalisch Technische Bundesanstalt (PTB) (Germany); Tiefenboeck, W. [Bundesamt fur Eich und Vermesungswesen (BEV) (Austria); Stucki, G. [17 Bundesamt fur Metrologie (METAS) (Switzerland); Van Dijk, E. [NMi Van Swinden Laboratorium (NMi) (Netherlands); Toni, M.P. [ENEA-CR Istituto Nazionale di Metrologia delle Radiazioni Ionizzanti (ENEA) (Italy); Minniti, R. [20 National Institute of Standards and Technology (NIST) (United States); McCaffrey, J.P. [National Research Council Canada (NRC) (Canada); Silva, C.N.M. [National Metrology Laboratory of Ionizing Radiation (LNMRI-IRD) (Brazil); Kharitonov, I. [D I Mendeleyev Institute for Metrology (VNIIM) (RU); Webb, D. [Australian Radiation Protection and Nuclear Safety Agency (ARPANSA) (Australia); Saravi, M. [National Atomic Energy Commission (CNEA-CAE) (Argentina); Delaunay, F. [Laboratoire National Henri Becquerel (LNE-LNHB) (France)

    2010-06-15

    The results of an unprecedented international effort involving 26 countries are reported. The EUROMET.RI(I)-K1 and EUROMET.RI(I)-K4 key comparisons were conducted with the goal of supporting the relevant calibration and measurement capabilities (CMC) planned for publication by the participant laboratories. The measured quantities were the air kerma (K{sub air}) and the absorbed dose to water (Dw) in {sup 60}Co radiotherapy beams. The comparison was conducted by the pilot laboratory MKEH (Hungary), in a star-shaped arrangement from January 2005 to December 2008. The calibration coefficients of four transfer ionization chambers were measured using two electrometers. The largest deviation between any two calibration coefficients for the four chambers in terms of air kerma and absorbed dose to water was 2.7% and 3.3% respectively. An analysis of the participant uncertainty budgets enabled the calculation of degrees of equivalence (DoE), in terms of the deviations of the results and their associated uncertainties. As a result of this EUROMET project 813 comparison, the BIPM key comparison database (KCDB) will include eleven new Kair and fourteen new D{sub w} DoE values of European secondary standard dosimetry laboratories (SSDLs), and the KCDB will be updated with the new DoE values of the other participant laboratories. The pair-wise degrees of equivalence of participants were also calculated. In addition to assessing calibration techniques and uncertainty calculations of the participants, these comparisons enabled the experimental determinations of N{sub Dw}/N{sub Kair} ratios in the {sup 60}Co gamma radiation beam for the four radiotherapy transfer chambers. (authors)

  8. THE VOLATILE COMPOSITION OF COMET C/2003 K4 (LINEAR) AT NEAR-IR WAVELENGTHS—COMPARISONS WITH RESULTS FROM THE NANÇAY RADIO TELESCOPE AND FROM THE ODIN, SPITZER, AND SOHO SPACE OBSERVATORIES

    Energy Technology Data Exchange (ETDEWEB)

    Paganini, L.; Mumma, M. J.; Villanueva, G. L.; DiSanti, M. A.; Bonev, B. P., E-mail: lucas.paganini@nasa.gov [Goddard Center for Astrobiology, NASA GSFC, MS 690, Greenbelt, MD 20771 (United States)

    2015-07-20

    We observed comet C/2003 K4 (LINEAR) using NIRSPEC at the Keck Observatory on UT 2004 November 28, when the comet was at 1.28 AU from the Sun (post-perihelion) and 1.38 AU from Earth. We detected six gaseous species (H{sub 2}O, OH*, C{sub 2}H{sub 6}, CH{sub 3}OH, CH{sub 4}, and HCN) and obtained upper limits for three others (H{sub 2}CO, C{sub 2}H{sub 2}, and NH{sub 3}). Our results indicate a water production rate of (1.72 ± 0.18) × 10{sup 29} molecules s{sup −1}, in reasonable agreement with production rates from SOHO (on the same day), Odin (one day earlier), and Nançay (about two weeks earlier). We also report abundances (relative to water) for seven trace species: CH{sub 3}OH (∼1.8%), CH{sub 4} (∼0.9%), and C{sub 2}H{sub 6} (∼0.4%) that were consistent with mean values among Oort cloud (OC) comets, while NH{sub 3} (<0.55%), HCN (∼0.07%), H{sub 2}CO (<0.07%), and C{sub 2}H{sub 2} (<0.04%) were “lower” than the mean values in other OC comets. We extracted inner-coma rotational temperatures for four species (H{sub 2}O, C{sub 2}H{sub 6}, CH{sub 3}OH, and CH{sub 4}), all of which are consistent with 70 K (within 1σ). The extracted ortho-para ratio for water was 3.0 ± 0.15, corresponding to spin temperatures larger than 39 K (at the 1σ level) and agreeing with those obtained with the Spitzer Space Telescope at the 2σ level.

  9. Micro–Positron Emission Tomography/Contrast-Enhanced Computed Tomography Imaging of Orthotopic Pancreatic Tumor–Bearing Mice Using the αvβ3 Integrin Tracer 64Cu-Labeled Cyclam-RAFT-c(-RGDfK-4

    Directory of Open Access Journals (Sweden)

    Winn Aung

    2013-09-01

    Full Text Available The purpose of this study was to develop a clinically relevant orthotopic xenotransplantation model of pancreatic cancer and to perform a preclinical evaluation of a new positron emission tomography (PET imaging probe, 64Cu-labeled cyclam-RAFT-c(-RGDfK-4 peptide (64Cu-RAFT-RGD, using this model. Varying degrees of αvβ3 integrin expression in several human pancreatic cancer cell lines were examined by flow cytometry and Western blotting. The cell line BxPC-3, which is stably transfected with a red fluorescence protein (RFP, was used for surgical orthotopic implantation. Orthotopic xenograft was established in the pancreas of recipient nude mice. An in vivo probe biodistribution and receptor blocking study, preclinical PET imaging coregistered with contrast-enhanced computed tomography (CECT comparing 64Cu-RAFT-RGD and 18F-fluoro-2-deoxy-D-glucose (18F-FDG accumulation in tumor, postimaging autoradiography, and histologic and immunohistochemical examinations were done. Biodistribution evaluation with a blocking study confirmed that efficient binding of probe to tumor is highly αvβ3 integrin specific. 64Cu-RAFT-RGD PET combined with CECT provided for precise and easy detection of cancer lesions. Autoradiography, histologic, and immunohistochemical examinations confirmed the accumulation of 64Cu-RAFT-RGD in tumor versus nontumor tissues. In comparative PET studies, 64Cu-RAFT-RGD accumulation provided better tumor contrast to background than 18F-FDG. Our results suggest that 64Cu-RAFT-RGD PET imaging is potentially applicable for the diagnosis of αvβ3 integrin–expressing pancreatic tumors.

  10. 635nm diode laser biostimulation on cutaneous wounds

    Science.gov (United States)

    Solmaz, Hakan; Gülsoy, Murat; Ülgen, Yekta

    2014-05-01

    Biostimulation is still a controversial subject in wound healing studies. The effect of laser depends of not only laser parameters applied but also the physiological state of the target tissue. The aim of this project is to investigate the biostimulation effects of 635nm laser irradiation on the healing processes of cutaneous wounds by means of morphological and histological examinations. 3-4 months old male Wistar Albino rats weighing 330 to 350 gr were used throughout this study. Low-level laser therapy was applied through local irradiation of red light on open skin excision wounds of 5mm in diameter prepared via punch biopsy. Each animal had three identical wounds on their right dorsal part, at which two of them were irradiated with continuous diode laser of 635nm in wavelength, 30mW of power output and two different energy densities of 1 J/cm2 and 3 J/cm2. The third wound was kept as control group and had no irradiation. In order to find out the biostimulation consequences during each step of wound healing, which are inflammation, proliferation and remodeling, wound tissues removed at days 3, 7, 10 and 14 following the laser irradiation are morphologically examined and than prepared for histological examination. Fragments of skin including the margin and neighboring healthy tissue were embedded in paraffin and 6 to 9 um thick sections cut are stained with hematoxylin and eosin. Histological examinations show that 635nm laser irradiation accelerated the healing process of cutaneous wounds while considering the changes of tissue morphology, inflammatory reaction, proliferation of newly formed fibroblasts and formation and deposition of collagen fibers. The data obtained gives rise to examine the effects of two distinct power densities of low-level laser irradiation and compare both with the non-treatment groups at different stages of healing process.

  11. Reduced nonlinearities in 100-nm high SOI waveguides

    Science.gov (United States)

    Lacava, C.; Marchetti, R.; Vitali, V.; Cristiani, I.; Giuliani, G.; Fournier, M.; Bernabe, S.; Minzioni, P.

    2016-03-01

    Here we show the results of an experimental analysis dedicated to investigate the impact of optical non linear effects, such as two-photon absorption (TPA), free-carrier absorption (FCA) and free-carrier dispersion (FCD), on the performance of integrated micro-resonator based filters for application in WDM telecommunication systems. The filters were fabricated using SOI (Silicon-on-Insulator) technology by CEA-Leti, in the frame of the FP7 Fabulous Project, which aims to develop low-cost and high-performance integrated optical devices to be used in new generation passive optical- networks (NG-PON2). Different designs were tested, including both ring-based structures and racetrack-based structures, with single-, double- or triple- resonator configuration, and using different waveguide cross-sections (from 500 x 200 nm to 825 x 100 nm). Measurements were carried out using an external cavity tunable laser source operating in the extended telecom bandwidth, using both continuous wave signals and 10 Gbit/s modulated signals. Results show that the use 100-nm high waveguide allows reducing the impact of non-linear losses, with respect to the standard waveguides, thus increasing by more than 3 dB the maximum amount of optical power that can be injected into the devices before causing significant non-linear effects. Measurements with OOK-modulated signals at 10 Gbit/s showed that TPA and FCA don't affect the back-to-back BER of the signal, even when long pseudo-random-bit-sequences (PRBS) are used, as the FCD-induced filter-detuning increases filter losses but "prevents" excessive signal degradation.

  12. Polishing Sapphire Substrates by 355 nm Ultraviolet Laser

    Directory of Open Access Journals (Sweden)

    X. Wei

    2012-01-01

    Full Text Available This paper tries to investigate a novel polishing technology with high efficiency and nice surface quality for sapphire crystal that has high hardness, wear resistance, and chemical stability. A Q-switched 355 nm ultraviolet laser with nanosecond pulses was set up and used to polish sapphire substrate in different conditions in this paper. Surface roughness Ra of polished sapphire was measured with surface profiler, and the surface topography was observed with scanning electronic microscope. The effects of processing parameters as laser energy, pulse repetition rate, scanning speed, incident angle, scanning patterns, and initial surface conditions on surface roughness were analyzed.

  13. Allsky Airglow Imagery from Albuquerque, NM; TOMEX 2000

    Science.gov (United States)

    Swenson, G. R.

    2001-12-01

    Allsky imager of OH Meinel and O2 Atmospheric emission bands were observed for the TOMEX rocket campaign from Albuquerque, NM, along with the Na/wind temperature lidar. Motion analysis of imagery describes a main wave propagating from the NW but other secondary waves appear from the NE to present a quasi chaotic wave field. The main wave had a typical horizontal wavelength of a several 10s of km and a period of less than15 minutes. The instrinsic wave parameters of the high frequency waves will be presented.

  14. A 65 nm CMOS LNA for Bolometer Application

    Science.gov (United States)

    Huang, Tom Nan; Boon, Chirn Chye; Zhu, Forest Xi; Yi, Xiang; He, Xiaofeng; Feng, Guangyin; Lim, Wei Meng; Liu, Bei

    2016-04-01

    Modern bolometers generally consist of large-scale arrays of detectors. Implemented in conventional technologies, such bolometer arrays suffer from integrability and productivity issues. Recently, the development of CMOS technologies has presented an opportunity for the massive production of high-performance and highly integrated bolometers. This paper presents a 65-nm CMOS LNA designed for a millimeter-wave bolometer's pre-amplification stage. By properly applying some positive feedback, the noise figure of the proposed LNA is minimized at under 6 dB and the bandwidth is extended to 30 GHz.

  15. Spectroscopy of Pluto, 380-930 Nm at Six Longitudes

    Science.gov (United States)

    Cruikshank, D. P.; Pinilla-Alonso, N.; Lorenzi, V.; Grundy, William; Licandro, J.; Binzel, R. P.

    2014-01-01

    We have obtained spectra of the Pluto-Charon pair (unresolved) in the wavelength range 380-930 nm with resolution approx..450 at six roughly equally spaced longitudes. The data were taken in May and June, 2014, with the 4.2-m Isaac Newton Telescope at Roque de Los Muchachos Observatory in the Canary Islands, using the ACAM (auxiliary-port camera) in spectrometer mode, and using two solar analog stars. The new spectra clearly show absorption bands of solid CH4 at 620, 728, and 850-910 nm, which were known from earlier work. The 620-nm CH4 band is intrinsically very weak, and its appearance indicates a long optical path-length through the ice. This is especially true if it arises from CH4 dissolved in N2 ice. Earlier work (Owen et al. Science 261, 745, 1993) on the near-infrared spectrum of Pluto (1-2.5 microns) has shown that the CH4 bands are shifted to shorter wavelengths because the CH4 occurs as a solute in beta-phase crystalline N2. The optical path-length through the N2 crystals must be on the order of several cm to produce the N2 band observed at 2.15 microns. The new spectra exhibit a pronounced red slope across the entire wavelength range; the slope is variable with longitude, and differs in a small but significant way from that measured at comparable longitudes by Grundy & Fink (Icarus 124, 329, 1996) in their 15-year study of Pluto's spectrum (500-1000 nm). The new spectra will provide an independent means for calibrating the color filter bands on the Multispectral Visible Imaging Camera (MVIC) (Reuter et al. Space Sci. Rev. 140, 129, 2008) on the New Horizons spacecraft, which will encounter the Pluto-Charon system in mid-2015. They will also form the basis of modeling the spectrum of Pluto at different longitudes to help establish the nature of the non-ice component(s) of Pluto's surface. It is presumed that the non-ice component is the source of the yellow-red coloration of Pluto, which is known to be variable across the surface.

  16. EST Table: NM_001046750 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001046750 LOC692907 10/09/29 94 %/284 aa ref|NP_001040215.1| stathmin [Bombyx mori] gb|ABD36259.1| stathm...GB18507-PA 10/09/10 47 %/338 aa gi|91083957|ref|XP_975021.1| PREDICTED: similar to stathmin [Tribolium castaneum] FS915193 ... ...in [Bombyx mori] 10/09/13 46 %/279 aa FBpp0144714|DgriGH10808-PA 10/08/29 n.h 10/09

  17. EST Table: NM_001184844 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001184844 TOR1 10/09/29 53 %/2461 aa ref|XP_625130.1| PREDICTED: similar to FKBP12-rapamycin... complex-associated protein (FK506-binding protein 12-rapamycin complex-associated protein 1) (R...apamycin target protein) (RAPT1) (Mammalian target of rapamycin) (MTOR) isoform 1 [Apis mellifera] 10/09/13 ...9/10 50 %/2441 aa gi|91089099|ref|XP_971819.1| PREDICTED: similar to fkbp-rapamycin associated protein [Tribolium castaneum] CK537623 ...

  18. High bit rate germanium single photon detectors for 1310nm

    Science.gov (United States)

    Seamons, J. A.; Carroll, M. S.

    2008-04-01

    There is increasing interest in development of high speed, low noise and readily fieldable near infrared (NIR) single photon detectors. InGaAs/InP Avalanche photodiodes (APD) operated in Geiger mode (GM) are a leading choice for NIR due to their preeminence in optical networking. After-pulsing is, however, a primary challenge to operating InGaAs/InP single photon detectors at high frequencies1. After-pulsing is the effect of charge being released from traps that trigger false ("dark") counts. To overcome this problem, hold-off times between detection windows are used to allow the traps to discharge to suppress after-pulsing. The hold-off time represents, however, an upper limit on detection frequency that shows degradation beginning at frequencies of ~100 kHz in InGaAs/InP. Alternatively, germanium (Ge) single photon avalanche photodiodes (SPAD) have been reported to have more than an order of magnitude smaller charge trap densities than InGaAs/InP SPADs2, which allowed them to be successfully operated with passive quenching2 (i.e., no gated hold off times necessary), which is not possible with InGaAs/InP SPADs, indicating a much weaker dark count dependence on hold-off time consistent with fewer charge traps. Despite these encouraging results suggesting a possible higher operating frequency limit for Ge SPADs, little has been reported on Ge SPAD performance at high frequencies presumably because previous work with Ge SPADs has been discouraged by a strong demand to work at 1550 nm. NIR SPADs require cooling, which in the case of Ge SPADs dramatically reduces the quantum efficiency of the Ge at 1550 nm. Recently, however, advantages to working at 1310 nm have been suggested which combined with a need to increase quantum bit rates for quantum key distribution (QKD) motivates examination of Ge detectors performance at very high detection rates where InGaAs/InP does not perform as well. Presented in this paper are measurements of a commercially available Ge APD

  19. Megasonic cleaning strategy for sub-10nm photomasks

    Science.gov (United States)

    Hsu, Jyh-Wei; Samayoa, Martin; Dress, Peter; Dietze, Uwe; Ma, Ai-Jay; Lin, Chia-Shih; Lai, Rick; Chang, Peter; Tuo, Laurent

    2016-10-01

    One of the main challenges in photomask cleaning is balancing particle removal efficiency (PRE) with pattern damage control. To overcome this challenge, a high frequency megasonic cleaning strategy is implemented. Apart from megasonic frequency and power, photomask surface conditioning also influences cleaning performance. With improved wettability, cleanliness is enhanced while pattern damage risk is simultaneously reduced. Therefore, a particle removal process based on higher megasonic frequencies, combined with proper surface pre-treatment, provides improved cleanliness without the unintended side effects of pattern damage, thus supporting the extension of megasonic cleaning technology into 10nm half pitch (hp) device node and beyond.

  20. EST Table: NM_001044005 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001044005 USP 10/09/29 84 %/462 aa ref|NP_001037470.1| protein ultraspiracle hom...olog [Bombyx mori] sp|P49700.1|USP_BOMMO RecName: Full=Protein ultraspiracle homolog; AltName: Full=Nuclear ...ene:AGAP002095 10/09/10 39 %/426 aa gnl|Amel|GB16648-PA 10/09/10 37 %/412 aa gi|121308144|emb|CAL25729.1| ultraspiracle nuclear receptor [Tribolium castaneum] AV400878 ...

  1. EST Table: NM_001046789 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001046789 LOC692949 10/09/29 95 %/267 aa ref|NP_001040254.1| beadex/dLMO protein... [Bombyx mori] gb|ABD36315.1| beadex/dLMO protein [Bombyx mori] 10/09/13 74 %/175 aa FBpp0262745|DyakGE17735...0 aa gnl|Amel|GB11268-PA 10/09/10 71 %/212 aa gi|91080717|ref|XP_975367.1| PREDICTED: similar to beadex/dLMO protein [Tribolium castaneum] FS794536 ...

  2. IFEL-Chicane Based Microbuncher at 800nm

    Energy Technology Data Exchange (ETDEWEB)

    Sears, C

    2004-09-15

    As a first stage to net acceleration in a laser based EM structure RF electron pulses must be microbunched to match the laser wavelength. We report on the design of an undulator and chicane for microbunching at 800nm using an inverse free electron laser (IFEL) interaction. This includes design considerations for the hardware itself, the laser IFEL interaction and bunching performance, and a full 3D particle tracking simulation to study the focusing effects and possible emittance growth due to the fringe fields of the magnets. The talk will close with a discussion of laser-electron beam diagnostics for overlap in the undulator and for diagnosing microbunching performance.

  3. Ocular safety limits for 1030nm femtosecond laser cataract surgery

    Science.gov (United States)

    Wang, Jenny; Sramek, Christopher; Paulus, Yannis M.; Lavinsky, Daniel; Schuele, Georg; Anderson, Dan; Dewey, David; Palanker, Daniel V.

    2013-03-01

    Application of femtosecond lasers to cataract surgery has added unprecedented precision and reproducibility but ocular safety limits for the procedure are not well-quantified. We present an analysis of safety during laser cataract surgery considering scanned patterns, reduced blood perfusion, and light scattering on residual bubbles formed during laser cutting. Experimental results for continuous-wave 1030 nm irradiation of the retina in rabbits are used to calibrate damage threshold temperatures and perfusion rate for our computational model of ocular heating. Using conservative estimates for each safety factor, we compute the limits of the laser settings for cataract surgery that optimize procedure speed within the limits of retinal safety.

  4. Design of the 65 nm CLICpix demonstrator chip

    CERN Document Server

    Valerio, P.; Campbell, M.

    2012-01-01

    A hybrid pixel detector ASIC designed to be used in the vertex detector for the CLIC experiment is presented in this note. It has been designed using a commercial 65 nm CMOS technology. The main features include simultaneous 4-bit TOT and TOA measurements with 10 ns accuracy, a spatial resolution of 3 um (the pixel size is 25x25 um), an on-chip data compression scheme and power pulsing capability. A prototype with a fully featured array of 64 by 64 pixels has been designed and produced. Testing on the prototype is ongoing.

  5. EST Table: NM_001123349 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001123349 18w 10/09/29 92 %/1295 aa ref|NP_001116821.1| 18 wheeler [Bombyx mori] dbj|BAB85498.1| 18 wheel...|Amel|GB15177-PA 10/09/10 58 %/1242 aa gi|91076478|ref|XP_972409.1| PREDICTED: similar to 18 wheeler [Tribolium castaneum] FS922922 ... ...er [Bombyx mori] 10/09/13 51 %/1248 aa FBpp0278316|DpseGA21191-PA 10/08/29 low homo

  6. 5 nm structures produced by direct laser writing

    Energy Technology Data Exchange (ETDEWEB)

    Pavel, E [Storex Technologies, Bucharest 020892 (Romania); Jinga, S; Andronescu, E; Vasile, B S [Department of Science and Engineering of Oxide Materials, Faculty of Applied Chemistry and Materials Science, University ' Politehnica' of Bucharest, Bucharest 011061 (Romania); Rotiu, E; Ionescu, L; Mazilu, C, E-mail: eugenp@rdslink.ro [National Glass Institute, Bucharest 032258 (Romania)

    2011-01-14

    Here we present a new approach to overcome the optical diffraction limit by using novel materials. In the paper, we report experimental results obtained by high-resolution transmission electron microscopy (HRTEM) and optical absorption spectroscopy, for a fluorescent photosensitive glass-ceramic containing rare-earth ions such as samarium (Sm). Using a home built dynamic tester, with a low power laser, we recorded nanostructures having 5 nm line widths. In the line structure, measurements reveal the presence of silver nanocrystals with few nanometre sizes. HRTEM shows that there is a random orientation of the nanocrystals. A writing mechanism with three steps is proposed.

  7. nm structures produced by direct laser writing.

    Science.gov (United States)

    Pavel, E; Jinga, S; Andronescu, E; Vasile, B S; Rotiu, E; Ionescu, L; Mazilu, C

    2011-01-14

    Here we present a new approach to overcome the optical diffraction limit by using novel materials. In the paper, we report experimental results obtained by high-resolution transmission electron microscopy (HRTEM) and optical absorption spectroscopy, for a fluorescent photosensitive glass-ceramic containing rare-earth ions such as samarium (Sm). Using a home built dynamic tester, with a low power laser, we recorded nanostructures having 5 nm line widths. In the line structure, measurements reveal the presence of silver nanocrystals with few nanometre sizes. HRTEM shows that there is a random orientation of the nanocrystals. A writing mechanism with three steps is proposed.

  8. Realization of 1.5 W 780 nm single-frequency laser by using cavity-enhanced frequency doubling of an EDFA boosted 1560 nm diode laser

    Science.gov (United States)

    Ge, Yulong; Guo, Shanlong; Han, Yashuai; Wang, Junmin

    2015-01-01

    We demonstrated a continuous-wave (CW) 780 nm laser with Watt-level output power by using external-cavity-enhanced frequency doubling of an Erbium-doped fiber amplifier (EDFA) boosted 1560 nm diode laser in a periodically-poled magnesium-oxide-doped lithium niobate (PPMgO:LN) bulk crystal. A 780 nm laser with maximum output power of 1.5 W is obtained at an incident 1560 nm laser of 2.05 W, corresponding to a doubling efficiency of 73%. The typical fluctuation of 780 nm laser's power is 1.2% (rms) in about 30 min. This 780 nm laser can be potentially applied to laser cooling and manipulation of rubidium atoms, generating 1560 nm squeezed vacuum field as well as 1560 nm continuous-variable entanglement based on optical parametric oscillator if additional locking and noise suppression is applied.

  9. 946 nm Diode Pumped Laser Produces 100mJ

    Science.gov (United States)

    Axenson, Theresa J.; Barnes, Norman P.; Reichle, Donald J., Jr.

    2000-01-01

    An innovative approach to obtaining high energy at 946 nm has yielded 101 mJ of laser energy with an optical-to-optical slope efficiency of 24.5%. A single gain module resonator was evaluated, yielding a maximum output energy of 50 mJ. In order to obtain higher energy a second gain module was incorporated into the resonator. This innovative approach produced un-surprised output energy of 101 mJ. This is of utmost importance since it demonstrates that the laser output energy scales directly with the number of gain modules. Therefore, higher energies can be realized by simply increasing the number of gain modules within the laser oscillator. The laser resonator incorporates two gain modules into a folded "M-shaped" resonator, allowing a quadruple pass gain within each rod. Each of these modules consists of a diode (stack of 30 microlensed 100 Watt diode array bars, each with its own fiber lens) end-pumping a Nd:YAG laser rod. The diode output is collected by a lens duct, which focuses the energy into a 2 mm diameter flat to flat octagonal pump area of the laser crystal. Special coatings have been developed to mitigate energy storage problems, including parasitic lasing and amplified spontaneous emission (ASE), and encourage the resonator to operate at the lower gain transition at 946 nm.

  10. High index fluoride materials for 193nm immersion lithography

    Science.gov (United States)

    Nawata, T.; Inui, Y.; Masada, I.; Nishijima, E.; Satoh, H.; Fukuda, T.

    2006-03-01

    We tried to investigate various kinds of metal fluoride materials which have higher gravity than CaF II and cubic crystal system, and we found out barium lithium fluoride (BaLiF 3) and potassium yttrium fluoride (KY 3F 10) as candidates for the last lens material. We have developed unique Czochralski (CZ) machines and techniques for the growth of large calcium fluoride single crystals. And we applied these technologies to the growth of fluoride high index materials. We have succeeded to grow the large BaLiF 3 single crystal with 120mm in diameter and a KY 3F 10 single crystal, and measured their basic properties such as refractive index, VUV transmittance, birefringence, and so on. As a result of our basic research, we found out that BaLiF 3 single crystal is transparent at VUV region, and the refractive index at 193nm is 1.64, and KY 3F 10 single crystal has the index of 1.59 at the wavelength of 193nm which is slightly higher than fused silica. We expect that these fluoride high index materials are useful for the last lens material of the next generation immersion lithography.

  11. Packaging and Performance of 980nm Broad Area Semiconductor Lasers

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    High power broad area semiconductor lasers have found increasing applications in pumping of solid state laser systems and fiber amplifiers, frequency doubling, medical systems and material processing.Packaging including the assembly design, process and thermal management, has a significant impact on the optical performance and reliability of a high power broad area laser. In this paper, we introduce the package structures and assembling process of 980nm broad area lasers and report the performances including output power, thermal behavior and far fields.We will report two types of high power broad area laser assemblies.One is a microchannel liquid cooled assembly and the other is a conduction cooled CT-mount assembly. Optical powers of 15W and 10W were achieved from a 980nm broad area laser with a 120 μ m stripe width in a microchannel liquid cooled assembly and conduction cooled CT-mount assembly, respectively.Furthermore,a high power of 6.5W out of fiber was demonstrated from a pigtailed, fully packaged butterfly-type module without TEC (Thermoelectric cooler).The measurement results showed that thermal management is the key in not only improving output power, but also significantly improving beam divergence and far field distribution.The results also showed that the die attach solder can significant impact the reliability of high power broad area lasers and that indium solder is not suitable for high power laser applications due to electromigration at high current densities and high temperatures.

  12. Efficient frequency doubling at 776 nm in a ring cavity

    Science.gov (United States)

    Han, Zhen-Hai; Liu, Shi-Long; Liu, Shi-Kai; Ding, Dong-Sheng; Zhou, Zhi-Yuan

    2017-08-01

    We report efficient frequency doubling (FD) at 776 nm using periodically poled LiNbO3 (PPLN) in a ring cavity pumped by a commercial erbium-doped fiber amplifier (EDFA) operating at 1552 nm. Two sets of input couplers are used that have been optimized to operate in the low pump and high pump regimes. The maximum conversion efficiencies measured for these couplers are 65.8% (transmittance T=4.5%) and 65.9% (T=9.1%). The internal conversion efficiencies are 85.0% and 88.2%, respectively, after the mode-matching efficiency and filtering transmittance have been taken into account. The maximum output powers obtained for the two couplers are 333 mW and 602 mW at pump powers of 535 mW and 999 mW, respectively. Coupling efficiency of more than 80% to single mode fibers indicates the high beam quality of the FD laser. This FD laser will be useful for quantum optics experiments in the telecommunications band and atomic physics experiments.

  13. Quality metric for accurate overlay control in <20nm nodes

    Science.gov (United States)

    Klein, Dana; Amit, Eran; Cohen, Guy; Amir, Nuriel; Har-Zvi, Michael; Huang, Chin-Chou Kevin; Karur-Shanmugam, Ramkumar; Pierson, Bill; Kato, Cindy; Kurita, Hiroyuki

    2013-04-01

    The semiconductor industry is moving toward 20nm nodes and below. As the Overlay (OVL) budget is getting tighter at these advanced nodes, the importance in the accuracy in each nanometer of OVL error is critical. When process owners select OVL targets and methods for their process, they must do it wisely; otherwise the reported OVL could be inaccurate, resulting in yield loss. The same problem can occur when the target sampling map is chosen incorrectly, consisting of asymmetric targets that will cause biased correctable terms and a corrupted wafer. Total measurement uncertainty (TMU) is the main parameter that process owners use when choosing an OVL target per layer. Going towards the 20nm nodes and below, TMU will not be enough for accurate OVL control. KLA-Tencor has introduced a quality score named `Qmerit' for its imaging based OVL (IBO) targets, which is obtained on the-fly for each OVL measurement point in X & Y. This Qmerit score will enable the process owners to select compatible targets which provide accurate OVL values for their process and thereby improve their yield. Together with K-T Analyzer's ability to detect the symmetric targets across the wafer and within the field, the Archer tools will continue to provide an independent, reliable measurement of OVL error into the next advanced nodes, enabling fabs to manufacture devices that meet their tight OVL error budgets.

  14. 1125-nm quantum dot laser for tonsil thermal therapy

    Science.gov (United States)

    McMillan, Kathleen

    2011-03-01

    Thermal therapy has the potential to provide a nonexcisional alternative to tonsillectomy. Clinical implementation requires that the lymphoid tissue of tonsils is heated homogeneously to produce an amount of primary thermal injury that corresponds to gradual postoperative tonsil shrinkage, with minimal risk of damage to underlying critical blood vessels. Optical constants are derived for tonsils from tissue components and used to calculate the depth of 1/e of irradiance. The 1125 nm wavelength is shown to correspond to both deep penetration and minimal absorption by blood. A probe for tonsil thermal therapy that comprises two opposing light emitting, temperature controlled surfaces is described. For ex vivo characterization of tonsil heating, a prototype 1125 nm diode laser is used in an experimental apparatus that splits the laser output into two components, and delivers the radiation to sapphire contact window surfaces of two temperature controlled cells arranged to irradiate human tonsil specimens from opposing directions. Temperatures are measured with thermocouple microprobes at located points within the tissue during and after irradiation. Primary thermal damage corresponding to the recorded thermal histories are calculated from Arrhenius parameters for human tonsils. Results indicate homogeneous heating to temperatures corresponding to the threshold of thermal injury and above can be achieved in advantageously short irradiation times.

  15. Fabrication of 70nm split ring resonators by nanoimprint lithography

    Science.gov (United States)

    Sharp, Graham J.; Khokhar, Ali Z.; Johnson, Nigel P.

    2012-05-01

    We report on the fabrication of 70 nm wide, high resolution rectangular U-shaped split ring resonators (SRRs) using nanoimprint lithography (NIL). The fabrication method for the nanoimprint stamp does not require dry etching. The stamp is used to pattern SRRs in a thin PMMA layer followed by metal deposition and lift-off. Nanoimprinting in this way allows high resolution patterns with a minimum feature size of 20 nm. This fabrication technique yields a much higher throughput than conventional e-beam lithography and each stamp can be used numerous times to imprint patterns. Reflectance measurements of fabricated aluminium SRRs on silicon substrates show a so-called an LC resonance peak in the visible spectrum under transverse electric polarisation. Fabricating the SRRs by NIL rather than electron beam lithography allows them to be scaled to smaller dimensions without any significant loss in resolution, partly because pattern expansion caused by backscattered electrons and the proximity effect are not present with NIL. This in turn helps to shift the magnetic response to short wavelengths while still retaining a distinct LC peak.

  16. Solar polarimetry through the K I lines at 770 nm

    Science.gov (United States)

    Quintero Noda, C.; Uitenbroek, H.; Katsukawa, Y.; Shimizu, T.; Oba, T.; Carlsson, M.; Orozco Suárez, D.; Ruiz Cobo, B.; Kubo, M.; Anan, T.; Ichimoto, K.; Suematsu, Y.

    2017-09-01

    We characterize the K I D1 & D2 lines in order to determine whether they could complement the 850 nm window, containing the Ca II infrared triplet lines and several Zeeman sensitive photospheric lines, that was studied previously. We investigate the effect of partial redistribution on the intensity profiles, their sensitivity to changes in different atmospheric parameters, and the spatial distribution of Zeeman polarization signals employing a realistic magnetohydrodynamic simulation. The results show that these lines form in the upper photosphere at around 500 km, and that they are sensitive to the line-of-sight velocity and magnetic field strength at heights where neither the photospheric lines nor the Ca II infrared lines are. However, at the same time, we found that their sensitivity to the temperature essentially comes from the photosphere. Then, we conclude that the K I lines provide a complement to the lines in the 850 nm window for the determination of atmospheric parameters in the upper photosphere, especially for the line-of-sight velocity and the magnetic field.

  17. Stress release during cyclic loading of 20 nm palladium films

    Energy Technology Data Exchange (ETDEWEB)

    Lukáč, František, E-mail: frantisek.lukac@mff.cuni.cz [Faculty of Mathematics and Physics, Charles University in Prague, V Holešovičkách 2, CZ-18000 Praha 8 (Czech Republic); Vlček, Marián; Vlach, Martin [Faculty of Mathematics and Physics, Charles University in Prague, V Holešovičkách 2, CZ-18000 Praha 8 (Czech Republic); Wagner, Stefan; Uchida, Helmut; Pundt, Astrid [Institute for Materials Physics, University of Göttingen, Friedrich-Hund-Platz 1, D-37077 Göttingen (Germany); Bell, Anthony [Deutsche Elektronen-Synchrotron (DESY), HASYLAB, Notkestrasse 85, D-2260 Hamburg (Germany); Čížek, Jakub [Faculty of Mathematics and Physics, Charles University in Prague, V Holešovičkách 2, CZ-18000 Praha 8 (Czech Republic)

    2015-10-05

    Highlights: • Repeated hydrogenation of 20 nm Pd films was investigated by in situ X-ray diffraction. • Hydride precipitates form coherent interfaces with matrix in nanocrystalline and epitaxial thin films. • Grain boundaries affect precipitation of the hydride phase in the nanocrystalline film. • Stress in epitaxial film is tensile due to different thermal expansion of Pd and sapphire. • After hydrogen absorption/desorption cycle the stress in both films becomes tensile. - Abstract: Gas phase loading of nanocrystalline and epitaxial 20 nm Pd films deposited on single crystalline sapphire substrates was studied in this work. The nanocrystalline film was deposited at room temperature and the epitaxial film deposited at 800 °C. The nanocrystalline film suffers from in-plane compressive stress imposed by atomic peening processes. The epitaxial film exhibits tensile stress caused by the different thermal expansion coefficients of Pd and sapphire substrate. Coherent phase transition into the hydride phase was observed both for the nanocrystalline and for the epitaxial film. For both films, the lattice parameters continuously increase during the phase transition to the hydride phase. Both films exhibit enhanced hydride formation pressure compared to bulk Pd. Misfit dislocations are formed at interface between Pd film and substrate during hydrogenation. This leads to irreversible change of stress state of the films subjected to sorption and desorption cycle with hydrogen.

  18. Alternating phase shift mask technology for 65nm logic applications

    Science.gov (United States)

    Chakravorty, Kishore K.; Henrichs, Sven; Qiu, Wei; Chavez, Joas L.; Liu, Yi-Ping; Ghadiali, Firoz; Yung, Karmen; Wilcox, Nathan; Silva, Mary; Ma, Jian; Qu, Ping; Irvine, Brian; Yun, Henry; Cheng, Wen-Hao; Farnsworth, Jeff

    2006-03-01

    Alternating Phase Shift Mask (APSM) Technology has been developed and successfully implemented for the poly gate of 65nm node Logic application at Intel. This paper discusses the optimization of the mask design rules and fabrication process in order to enable high volume manufacturability. Intel's APSM technology is based on a dual sided trenched architecture. To meet the stringent OPC requirements associated with patterning of narrow gates required for the 65nm node, Chrome width between the Zero and Pi aperture need to be minimized. Additionally, APSM lithography has an inherently low MEEF that furthermore, drives a narrower Chrome line as compared to the Binary approach. The double sided trenched structure with narrow Chrome lines are mechanically vulnerable and prone to damage when exposed to conventional mask processing steps. Therefore, new processing approaches were developed to minimize the damage to the patterned mask features. For example, cleaning processes were optimized to minimize Chrome & quartz damage while retaining the cleaning effectiveness. In addition, mask design rules were developed which ensured manufacturability. The narrow Chrome regions between the zero and Pi apertures severely restrict the tolerance for the placement of the second level resists edges with respect to the first level. UV Laser Writer based resist patterning capability, capable of providing the required Overlay tolerance, was developed, An AIMS based methodology was used to optimize the undercut and minimize the aerial image CD difference between the Zero and Pi apertures.

  19. On the validity of the 630 nm Fe I nm lines for the magnetometry of the internetwork quiet Sun

    CERN Document Server

    Gonzalez, M J M; Cobo, B R

    2006-01-01

    The purpose of this work is to analyze the reliability of the magnetic field strengths inferred from the 630 nm pair of Fe I lines at internetwork quiet Sun regions. Some numerical experiments have been performed that demonstrate the inability of these lines to recover the magnetic field strength in such low flux solar regions. It is shown how different model atmospheres, with magnetic field strengths ranging from few hundred Gauss to kiloGauss, give rise to Stokes profiles that can not be distinguished. The reasons for this degeneracy are discussed.

  20. Holistic overlay control for multi-patterning process layers at the 10nm and 7nm nodes

    Science.gov (United States)

    Verstappen, Leon; Mos, Evert; Wardenier, Peter; Megens, Henry; Schmitt-Weaver, Emil; Bhattacharyya, Kaustuve; Adam, Omer; Grzela, Grzegorz; van Heijst, Joost; Willems, Lotte; Wildenberg, Jochem; Ignatova, Velislava; Chen, Albert; Elich, Frank; Rajasekharan, Bijoy; Vergaij-Huizer, Lydia; Lewis, Brian; Kea, Marc; Mulkens, Jan

    2016-03-01

    Multi-patterning lithography at the 10-nm and 7-nm nodes is driving the allowed overlay error down to extreme low values. Advanced high order overlay correction schemes are needed to control the process variability. Additionally the increase of the number of split layers results in an exponential increase of metrology complexity of the total overlay and alignment tree. At the same time, the process stack includes more hard-mask steps and becomes more and more complex, with as consequence that the setup and verification of the overlay metrology recipe becomes more critical. All of the above require a holistic approach that addresses total overlay optimization from process design to process setup and control in volume manufacturing. In this paper we will present the holistic overlay control flow designed for 10-nm and 7-nm nodes and illustrate the achievable ultimate overlay performance for a logic and DRAM use case. As figure 1 illustrates we will explain the details of the steps in the holistic flow. Overlay accuracy is the driver for target design and metrology tool optimization like wavelength and polarization. We will show that it is essential to include processing effects like etching and CMP which can result in a physical asymmetry of the bottom grating of diffraction based overlay targets. We will introduce a new method to create a reference overlay map, based on metrology data using multiple wavelengths and polarization settings. A similar approach is developed for the wafer alignment step. The overlay fingerprint correction using linear or high order correction per exposure (CPE) has a large amount of parameters. It is critical to balance the metrology noise with the ultimate correction model and the related metrology sampling scheme. Similar approach is needed for the wafer align step. Both for overlay control as well as alignment we have developed methods which include efficient use of metrology time, available for an in the litho-cluster integrated

  1. DNA methylation and not H3K4 trimethylation dictates the expression status of miR-152 gene which inhibits migration of breast cancer cells via DNMT1/CDH1 loop.

    Science.gov (United States)

    Sengupta, Dipta; Deb, Moonmoon; Rath, Sandip Kumar; Kar, Swayamsiddha; Parbin, Sabnam; Pradhan, Nibedita; Patra, Samir Kumar

    2016-08-15

    MicroRNAs (miRNA) are small non-coding RNAs which targets most protein-coding transcripts (mRNA) and destroy them. Thus miRNA controls the abundance of those specific proteins and impact on developmental, physiological and pathological processes. Dysregulation of miRNA function thus may lead to various clinicopathological complications, including breast cancer. Silencing of miR-152 gene due to promoter DNA methylation alter the expression pattern of several other genes. E-cadherin (CDH1) forms the core of adherent junctions between surrounding epithelial cells, link with actin cytoskeleton and affects cell signaling. CDH1 gene is down regulated by promoter DNA methylation during cancer progression. In this investigation, we attempt to elucidate the correlation of miR-152 and CDH1 function, as it is well known that the loss of CDH1 function is one of the major reasons for cancer metastasis and aggressiveness of spreading. For the first time we have shown that loss of CDH1 expression is directly proportional to the loss of miR-152 function in breast cancer cells. mRNA and protein expression profile of DNMT1 implicate that miR-152 targets DNMT1 mRNA and inhibits its protein expression. Tracing the molecular marks on DNA and histone 3 for understanding the mechanism of gene regulation by ChIP analyses leads to a paradoxical result that shows DNA methylation adjacent to active histone marking (enrichment of H3K4me3) silence miR-152 gene. Further experiments revealed that DNMT1 plays crucial role for regulation of miR-152 gene. When DNMT1 protein function is blocked miR-152 expression prevails and destroys the mRNA of DNMT1; this molecular regulatory mechanism is creating a cyclic feedback loop, which is now focused as DNMT1/miR-152 switch for on/off of DNMT1 target genes. We discovered modulation of CDH1 gene expression by DNMT1/miR-152 switches. We have demonstrated further that DNMT1 down regulation mediated upregulation of CDH1 (hereafter, DNMT1/CDH1 loop) in

  2. CCPR-S1 Supplementary comparison for spectral radiance in the range of 220 nm to 2500 nm

    Science.gov (United States)

    Khlevnoy, Boris; Sapritsky, Victor; Rougie, Bernard; Gibson, Charles; Yoon, Howard; Gaertner, Arnold; Taubert, Dieter; Hartmann, Juergen

    2009-08-01

    In 1997, the Consultative Committee for Photometry and Radiometry (CCPR) initiated a supplementary comparison of spectral radiance in the wavelength range from 220 nm to 2500 nm (CCPR-S1) using tungsten strip-filament lamps as transfer standards. Five national metrology institutes (NMIs) took part in the comparison: BNM/INM (France), NIST (USA), NRC (Canada), PTB (Germany) and VNIIOFI (Russia), with VNIIOFI as the pilot laboratory. Each NMI provided the transfer lamps that were used to transfer their measurements to the pilot laboratory. The intercomparison sequence began with the participant measurements, then the pilot measurements, followed by a second set of measurements by the participant laboratory. The measurements were carried out from 1998 to 2002, with the final report completed in 2008. This paper presents the descriptions of measurement facilities and uncertainties of the participants, as well as the comparison results that were analysed in accordance with the Guidelines for CCPR Comparisons Report Preparation, and a re-evaluation of the results taking into account the instability of some of the transfer lamps. Excluding a few wavelengths, all participants agree with each other within ±1.5%. The disagreement decreases to approximately ±1.0% when the anomalous data are excluded from the analysis.

  3. Final report on EUROMET PR-K2.b: Comparison on spectral responsivity (300 nm to 1000 nm)

    Science.gov (United States)

    Campos, Joaquin; Pons, Alicia; Blattner, Peter; Dubard, Jimmy; Bastie, Jean; Litwiniuk, Lukasz; Pietrzykowski, Jerzy; Smid, Marek; Mihai, Sim; Bos, Daniel; Gran, Jarle; Bazkir, Ozcan; Fäldt, Anne A.

    2013-01-01

    This report contains the results of the regional comparison EUROMET PR-K2.b (registered in the KCDB under the identifier EURAMET.PR-K2.b). Ten laboratories took part in it, including the pilot. In general the results are consistent, with a few exceptions as explained in the report. The comparison gives international linkage in spectral responsivity from 300 nm to 1000 nm to seven European laboratories: Bundesamt für Metrologie und Akkreditierung (METAS), Norwegian Metrology and Accreditation Service (Justervesenet), Central Office of Measures (GUM), National Institute of Metrology (INM-Romania), Optics Laboratory of TUBITAK-UME (UME), Czech Metrology Institute (CMI) and Swedish National Testing and Research Institute (SP). Three laboratories provided the link to CCPR-K2.b: Bureau National de Metrologie (BNM-INM/CNAM), Instituto de Optica 'Daza de Valdés' (IO-CSIC, acting as pilot) and NMi Van Swinden Laboratorium BV (NMi-VSL). Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCPR, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  4. Photoinitiated oxidation of geosmin and 2-methylisoborneol by irradiation with 254 nm and 185 nm UV light.

    Science.gov (United States)

    Kutschera, Kristin; Börnick, Hilmar; Worch, Eckhard

    2009-05-01

    The degradation of geosmin and 2-methylisoborneol (2-MIB) by UV irradiation at different wavelengths was investigated under varying boundary conditions. The results showed that conventional UV radiation (254 nm) is ineffective in removing these compounds from water. In contrast to the usual UV radiation UV/VUV radiation (254+185 nm) was more effective in the removal of the taste and odour compounds. The degradation could be described by a simple pseudo first-order rate law with rate constants of about 1.2 x 10(-3) m(2)J(-1) for geosmin and 2-MIB in ultrapure water. In natural water used for drinking water abstraction the rate constants decreased to 2.7 x 10(-4) m(2)J(-1) for geosmin and 2.5 x 10(-4) m(2)J(-1) for 2-MIB due to the presence of NOM. Additionally, the formation of the by-product nitrite was studied. In the UV/VUV irradiation process up to 0.6 mg L(-1) nitrite was formed during the complete photoinitiated oxidation of the odour compounds. However, the addition of low ozone doses could prevent the formation of nitrite in the UV/VUV irradiation experiments.

  5. Comparison of the effects of 60 nm and 96 nm thick patterned permalloy thin films on the performance of on-chip spiral inductors

    Science.gov (United States)

    Pulijala, Vasu; Syed, Azeemuddin

    2016-12-01

    In our earlier work it was shown that the 60 nm domain patterned Permalloy, incorporated on on-chip spiral inductors, gave better performance at frequencies greater than 5 GHz compared to the bulk Permalloy incorporated inductors, and the control structure. In this paper we compare the effects of 60 nm and 96 nm thick domain patterned Permalloy thin films, on the performance of on-chip spiral inductors. Experimental results show that the 60 nm thick both bulk and patterned Permalloys provide more improvement in inductance and quality factor of inductors, compared to that of 96 nm.

  6. 65 nm CMOS Sensors Applied to Mathematically Exact Colorimetric Reconstruction

    CERN Document Server

    Mayr, C; Krause, A; Schlüßler, J -U; Schüffny, R

    2014-01-01

    Extracting colorimetric image information from the spectral characteristics of image sensors is a key issue in accurate image acquisition. Technically feasible filter/sensor combinations usually do not replicate colorimetric responses with sufficient accuracy to be directly applicable to color representation. A variety of transformations have been proposed in the literature to compensate for this. However, most of those rely on heuristics and/or introduce a reconstruction dependent on the composition of the incoming illumination. In this work, we present a spectral reconstruction method that is independent of illumination and is derived in a mathematically strict way. It provides a deterministic method to arrive at a least mean squared error approximation of a target spectral characteristic from arbitrary sensor response curves. Further, we present a new CMOS sensor design in a standard digital 65nm CMOS technology. Novel circuit techniques are used to achieve performance comparable with much larger-sized spe...

  7. Vanadium dioxide spatial light modulator for applications beyond 1200 nm

    Science.gov (United States)

    Anh Do, Phuong; Hendaoui, Ali; Mortazy, Ebrahim; Chaker, Mohamed; Haché, Alain

    2013-02-01

    Spatial light modulators based on vanadium dioxide are used to demonstrate all-optical spectral filtering in the near infrared, up to 1700 nm, with potential to application into the mid-infrared. By spectrally dispersing the shaped beam and transmitting the beam through a vanadium dioxide thin film, the transmission is modified by optically pumping the film locally with a laser beam. Heating causes the film to undergo an insulator-to-metal transition, along with a drop in transmission. The spectrum can be shaped by pumping with a beam at different location and/or different intensity profiles. The method is promising for longer wavelength since the film is more efficient further in the infrared.

  8. NM-Scale Anatomy of an Entire Stardust Carrot Track

    Science.gov (United States)

    Nakamura-Messenger, K.; Keller, L. P.; Clemett, S. J.; Messenger, S.

    2009-01-01

    Comet Wild-2 samples collected by NASA s Stardust mission are extremely complex, heterogeneous, and have experienced wide ranges of alteration during the capture process. There are two major types of track morphologies: "carrot" and "bulbous," that reflect different structural/compositional properties of the impactors. Carrot type tracks are typically produced by compact or single mineral grains which survive essentially intact as a single large terminal particle. Bulbous tracks are likely produced by fine-grained or organic-rich impactors [1]. Owing to their challenging nature and especially high value of Stardust samples, we have invested considerable effort in developing both sample preparation and analytical techniques tailored for Stardust sample analyses. Our report focuses on our systematic disassembly and coordinated analysis of Stardust carrot track #112 from the mm to nm-scale.

  9. Pollution Prevention Opportunity Assessment for the SNL/NM cafeterias.

    Energy Technology Data Exchange (ETDEWEB)

    McCord, Samuel Adam

    2005-12-01

    This Pollution Prevention Opportunity Assessment (PPOA) was conducted for the two Sandia National Laboratories/New Mexico cafeteria facilities between May and August 2005. The primary purpose of this PPOA is to assess waste and resource reduction opportunities and issue Pollution Prevention (P2) recommendations for Sandia's food service facilities. This PPOA contains recommendations for energy, water and resource reduction, as well as material substitution based upon environmentally preferable purchasing. Division 3000 has requested the PPOA report as part of the Division's compliance effort to implement the Environmental Management System (EMS) per DOE Order 450.1. This report contains a summary of the information collected and analyses performed with recommended options for implementation. The SNL/NM P2 Group will work with Division 3000 and the respective cafeteria facilities to implement these options.

  10. Pixel front-end development in 65 nm CMOS technology

    CERN Document Server

    Havránek, M; Kishishita, T; Krüger, H; Wermes, N

    2014-01-01

    Luminosity upgrade of the LHC (HL-LHC) imposes severe constraints on the detector tracking systems in terms of radiation hardness and capability to cope with higher hit rates. One possible way of keeping track with increasing luminosity is the usage of more advanced technologies. Ultra deep sub-micron CMOS technologies allow a design of complex and high speed electronics with high integration density. In addition, these technologies are inherently radiation hard. We present a prototype of analog pixel front-end integrated circuit designed in 65 nm CMOS technology with applications oriented towards the ATLAS Pixel Detector upgrade. The aspects of ultra deep sub-micron design and performance of the analog pixel front-end circuits will be discussed.

  11. Progress in development of a monolithic 680-nm MOPA

    Science.gov (United States)

    Pezeshki, Bardia; Osinski, Jules S.; Zhao, Hanmin; Mathur, Atul

    1997-05-01

    The various components for red MOPAs (Master Oscillator Power Amplifier) have been demonstrated and exhibit excellent performance. A major impediment has been the regrowth over material with high aluminum concentration, necessary for short wavelength operation. Nevertheless, amplifiers, DFB, and DBR lasers have been demonstrated in discrete form. Single frequency DBR and DFB lasers using a buried diffraction grating emit over 20 mW with efficiencies of up to 0.4 W/A. The DBR can be tuned over 3 nm using current injection in the grating, and preliminary lifetests indicate good reliability. Discrete flared amplifiers exhibited nearly 1.6 W pulsed, and 500 mW CW output power. The performance of the individual devices and integration issues in developing the MOPA will be discussed.

  12. Extreme ultraviolet resist materials for sub-7 nm patterning

    KAUST Repository

    Li, Li

    2017-06-26

    Continuous ongoing development of dense integrated circuits requires significant advancements in nanoscale patterning technology. As a key process in semiconductor high volume manufacturing (HVM), high resolution lithography is crucial in keeping with Moore\\'s law. Currently, lithography technology for the sub-7 nm node and beyond has been actively investigated approaching atomic level patterning. EUV technology is now considered to be a potential alternative to HVM for replacing in some cases ArF immersion technology combined with multi-patterning. Development of innovative resist materials will be required to improve advanced fabrication strategies. In this article, advancements in novel resist materials are reviewed to identify design criteria for establishment of a next generation resist platform. Development strategies and the challenges in next generation resist materials are summarized and discussed.

  13. Megasonic cleaning: possible solutions for 22nm node and beyond

    Science.gov (United States)

    Shende, Hrishi; Singh, Sherjang; Baugh, James; Mann, Raunak; Dietze, Uwe; Dress, Peter

    2011-11-01

    Megasonic energy transfer to the photomask surface is indirectly controlled by process parameters that provide an effective handle to physical force distribution on the photomask surface. A better understanding of the influence of these parameters on the physical force distribution and their effect on pattern damage of fragile mask features can help optimize megasonic energy transfer as well as assist in extending this cleaning technology beyond the 22nm node. In this paper we have specifically studied the effect of higher megasonic frequencies (3 & 4MHz) and media gasification on pattern damage; the effect of cleaning chemistry, media volume flow rate, process time, and nozzle distance to the mask surface during the dispense is also discussed. Megasonic energy characterization is performed by measuring the acoustic energy as well as cavitation created by megasonic energy through sonoluminescence measurements.

  14. Laser lipolysis with a 980 nm diode laser.

    Science.gov (United States)

    Forman Taub, Amy; Friedman, Adam

    2010-05-01

    Laser lipolysis is recognized as an effective, non-surgical solution for fat removal and body reshaping. Its appeal lay in the procedure's ability to treat localized fat deposits and correct body asymmetries with apparent decreased risk compared to traditional liposuction. The energy emitted by the laser uses volumetric heating to destroy fat cells, contract skin and stimulate collagenesis. Although devices of five different wavelengths are FDA approved for lipolysis, it has been found that the 980 nm diode laser is consistently successful in inducing the required fat-heat and skin-heat interactions necessary for optimal results. Although laser lipolysis is not intended to replace traditional liposuction, it offers patients a procedure that yields similar benefits with fewer complications and faster recovery.

  15. Photofragment translational spectroscopy of iodobenzene at 266 nm

    Institute of Scientific and Technical Information of China (English)

    张宏; 朱荣顺; 王光俊; 孙巨龙; 韩克利; 何国钟; 楼南泉

    1999-01-01

    The photofragmentation of C6 H5I at 266 nm is investigated on the nuiversal crossed molecular beam machine, and the translational spectroseopy as well as the angular distribution of I atom is measured. The results reveal that under the laser intensity of 108 W/cm2 the single-phuton dissociation competes with multi-photon processes. In singlephoton dissociation the anisotropy parameter β is 0.4 and the average translational energy is only 1.04 keal/mol, which indicates that this process is a slow predissociation. In two-photon phutofragmentation the average translational energy is 51.64 kcal/mol, which accounts for about 35% of the available energy. Another photofragmentation channel is even more faster, whose peak in time-of-flight spectra corresponds to four or five photon absorptions. The branching ratio of these three channels is determined to be about 3: 3: 4.

  16. Search for solar neutrons using NM-64 equipment

    Science.gov (United States)

    Martinic, N. J.; Reguerin, A.; Palenque, E.; Taquichiri, M. A.; Wada, M.; Inoue, A.; Takahashi, K.

    1985-01-01

    Two years (1980 to 1982) neutron monitor data from the Chacaltaya (geographic coordinates: N16.32 deg W68. 15 deg; cutoff rigidity: 13.1 GV; altitude: 5,300 m a.s.l.) station has been scanned; the sampling time of the 12NM-64 neutron monitor is 5 min. The nucleonic component increases have been correlated with 66 hard X-, gamma rays satellite data from solar origin, as reported by several groups. Typical neutron monitor time profiles of the events are presented. Chree-analysis was performed discriminating the events according to its solar coordinates. Ground data from solar limb locii are more enhanced at the time of the onset than other geometrically visible flares. Chree histograms of neutron monitor output profiles are also presented from geometrically invisible events from the Chacaltaya station.

  17. Wide modulation bandwidth terahertz detection in 130 nm CMOS technology

    Science.gov (United States)

    Nahar, Shamsun; Shafee, Marwah; Blin, Stéphane; Pénarier, Annick; Nouvel, Philippe; Coquillat, Dominique; Safwa, Amr M. E.; Knap, Wojciech; Hella, Mona M.

    2016-11-01

    Design, manufacturing and measurements results for silicon plasma wave transistors based wireless communication wideband receivers operating at 300 GHz carrier frequency are presented. We show the possibility of Si-CMOS based integrated circuits, in which by: (i) specific physics based plasma wave transistor design allowing impedance matching to the antenna and the amplifier, (ii) engineering the shape of the patch antenna through a stacked resonator approach and (iii) applying bandwidth enhancement strategies to the design of integrated broadband amplifier, we achieve an integrated circuit of the 300 GHz carrier frequency receiver for wireless wideband operation up to/over 10 GHz. This is, to the best of our knowledge, the first demonstration of low cost 130 nm Si-CMOS technology, plasma wave transistors based fast/wideband integrated receiver operating at 300 GHz atmospheric window. These results pave the way towards future large scale (cost effective) silicon technology based terahertz wireless communication receivers.

  18. Multiphoton ionization of acetone-water clusters at 355 nm

    Institute of Scientific and Technical Information of China (English)

    WANG Reng; KONG Xiang-he; ZHANG Shu-dong; ZHANG Xia; FAN Xing-yan; ZHAO Shu-yan

    2006-01-01

    @@ The multiphoton ionization of acetone-water clusters were detected at 355 nm laser wavelength by using the time of flight mass spectrometer(TOF-MS).The experiments show that all products are protonated.Three main products such as (CH3COCH3)n-(H2O)n-2H+,(CH3COCH3)n-(H2O)n-1H+ and (CH3COCH3)n-(H2O)nH+ are concluded from the results.In order to study the equilibrium structures of the (CH3COCH3)n-(H2O)n-2H+,the ab-initio calculation is used on them.The experiment is even done when the volume rate of acetone to water is 1:2.

  19. EST Table: NM_001111334 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available NM_001111334 Br-c 10/09/29 81 %/420 aa ref|NP_001104804.1| broad-complex isoform Z1... [Bombyx mori] dbj|BAD23978.1| broad-complex Z1-isoform [Bombyx mori] dbj|BAD23983.1| broad-complex Z1-isofo...rm [Bombyx mori] dbj|BAD24045.1| Broad-Complex isoform Z1 [Bombyx mori] dbj|BAD24046.1| Broad-Complex isofor...m Z1 [Bombyx mori] dbj|BAD46732.1| broad-complex A-Z1 isoform [Bombyx mori] dbj|BAD46739.1| broad...-complex B-Z1 isoform [Bombyx mori] dbj|BAF43564.1| Broad-Complex isoform Z1 [Bombyx mori] 1

  20. Spectral narrowing of a 980 nm tapered diode laser bar

    DEFF Research Database (Denmark)

    Vijayakumar, Deepak; Jensen, Ole Bjarlin; Lucas Leclin, Gaëlle

    2011-01-01

    High power diode laser bars are interesting in many applications such as solid state laser pumping, material processing, laser trapping, laser cooling and second harmonic generation. Often, the free running laser bars emit a broad spectrum of the order of several nanometres which limit their scope...... in wavelength specific applications and hence, it is vital to stabilize the emission spectrum of these devices. In our experiment, we describe the wavelength narrowing of a 12 element 980 nm tapered diode laser bar using a simple Littman configuration. The tapered laser bar which suffered from a big smile has...... been "smile corrected" using individual phase masks for each emitter. The external cavity consists of the laser bar, both fast and slow axis micro collimators, smile correcting phase mask, 6.5x beam expanding lens combination, a 1200 lines/mm reflecting grating with 85% efficiency in the first order...